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Sample records for agricultural biogas plants

  1. Methane emissions from digestate at an agricultural biogas plant.

    PubMed

    Baldé, Hambaliou; VanderZaag, Andrew C; Burtt, Stephen D; Wagner-Riddle, Claudia; Crolla, Anna; Desjardins, Raymond L; MacDonald, Douglas J

    2016-09-01

    Methane (CH4) emissions were measured over two years at an earthen storage containing digestate from a mesophilic biodigester in Ontario, Canada. The digester processed dairy manure and co-substrates from the food industry, and destroyed 62% of the influent volatile solids (VS). Annual average emissions were 19gCH4m(-3)d(-1) and 0.27gCH4kg(-1)VSd(-1). About 76% of annual emissions occurred from June to October. Annual cumulative emissions from digestate corresponded to 12% of the CH4 produced within the digester. A key contributor to CH4 emissions was the sludge layer in storage, which contained as much VS as the annual discharge from the digester. These findings suggest that digestate management provides an opportunity to further enhance the benefits of biogas (i.e. reducing CH4 emissions compared to undigested liquid manure, and producing renewable energy). Potential best practices for future study include complete storage emptying, solid-liquid separation, and storage covering. PMID:27323243

  2. Degradation efficiency of agricultural biogas plants--a full-scale study.

    PubMed

    Ruile, Stephan; Schmitz, Sabine; Mönch-Tegeder, Matthias; Oechsner, Hans

    2015-02-01

    The degradation efficiency of 21 full-scale agricultural CSTR biogas plants was investigated. The residual methane potential of the digestion stages was determined in batch digestion tests (20.0 and 37.0 °C). The results of this study showed that the residual methane yield is significantly correlated to the HRT (r=-0.73). An almost complete degradation of the input substrates was achieved due to a HRT of more than 100 days (0.097±0.017 Nm(3)/kg VS). The feedstock characteristics have the largest impact to the degradation time. It was found that standard values of the methane yield are a helpful tool for evaluating the degradation efficiency. Adapting the HRT to the input materials is the key factor for an efficient degradation in biogas plants. No influence of digester series configuration to the VS degradation was found. The mean VS degradation rate in the total reactor systems was 78±7%. PMID:25453437

  3. The use of biogas plant fermentation residue for the stabilisation of toxic metals in agricultural soils

    NASA Astrophysics Data System (ADS)

    Geršl, Milan; Šotnar, Martin; Mareček, Jan; Vítěz, Tomáš; Koutný, Tomáš; Kleinová, Jana

    2015-04-01

    Our department has been paying attention to different methods of soil decontamination, including the in situ stabilisation. Possible reagents to control the toxic metals mobility in soils include a fermentation residue (FR) from a biogas plant. Referred to as digestate, it is a product of anaerobic decomposition taking place in such facilities. The fermentation residue is applied to soils as a fertiliser. A new way of its use is the in situ stabilisation of toxic metals in soils. Testing the stabilisation of toxic metals made use of real soil samples sourced from five agriculturally used areas of the Czech Republic with 3 soil samples taken from sites contaminated with Cu, Pb and Zn and 2 samples collected at sites of natural occurrence of Cu, Pb and Zn ores. All the samples were analysed using the sequential extraction procedure (BCR) (determine the type of Cu, Pb and Zn bonds). Stabilisation of toxic metals was tested in five soil samples by adding reagents as follows: dolomite, slaked lime, goethite, compost and fermentation residue. A single reagent was added at three different concentrations. In the wet state with the added reagents, the samples were left for seven days, shaken twice per day. After seven days, metal extraction was carried out: samples of 10 g soil were shaken for 2 h in a solution of 0.1M NH4NO3 at a 1:2.5 (g.ml-1), centrifuged for 15 min at 5,000 rpm and then filtered through PTFE 0.45 μm mesh filters. The extracts were analysed by ICP-OES. Copper The best reduction of Cu concentration in the extract was obtained at each of the tested sites by adding dolomite (10 g soil + 0.3 g dolomite). The concentration of Cu in the leachate decreased to 2.1-18.4% compare with the leachate without addition. Similar results were also shown for the addition of fermentation residue (10 g soil + 1 g FR). The Cu concentration in the leachate decreased to 16.7-26.8% compared with the leachate without addition. Lead The best results were achieved by adding

  4. Shell structures for biogas plants

    SciTech Connect

    Sasse, L.

    1982-01-01

    The shell structures designed for biogas plants of the fixed-dome type by the Bremen Overseas Research and Development Association are described. Biogas digesters of the design described have been successfully tested in Rwanda and India without structural or contractural problems.

  5. Community shifts in a well-operating agricultural biogas plant: how process variations are handled by the microbiome.

    PubMed

    Theuerl, Susanne; Kohrs, Fabian; Benndorf, Dirk; Maus, Irena; Wibberg, Daniel; Schlüter, Andreas; Kausmann, Robert; Heiermann, Monika; Rapp, Erdmann; Reichl, Udo; Pühler, Alfred; Klocke, Michael

    2015-09-01

    This study provides a comprehensive, long-term microbiological study of a continuously operated, mesophilic, agricultural biogas plant fed with whole-crop silages of maize and rye, cattle manure and cattle slurry. The microbial community structure was accessed by high-throughput 16S rRNA gene amplicon sequencing. For the characterisation of the microbial dynamics, the community profiling method terminal restriction fragment length polymorphism (TRFLP) in combination with a cloning-sequencing approach as well as a LC-MS/MS approach for protein identification were applied. Our results revealed that the anaerobic digestion is a highly sensitive process: small variations in the process performance induce fluctuations in the microbial community composition and activity. In this context, it could be proven that certain microbial species were better adapted to changing process condition such as temperature (interspecies competition) and that there is a physiological compensation between different microorganisms so that the reactor efficiency was not adversely affected despite of structural and functional changes within the microbial community. PMID:25998656

  6. Analysis of operational methane emissions from pressure relief valves from biogas storages of biogas plants.

    PubMed

    Reinelt, Torsten; Liebetrau, Jan; Nelles, Michael

    2016-10-01

    The study presents the development of a method for the long term monitoring of methane emissions from pressure relief valves (PRV(1)) of biogas storages, which has been verified during test series at two PRVs of two agricultural biogas plants located in Germany. The determined methane emission factors are 0.12gCH4kWhel(-1) (0.06% CH4-loss, within 106days, 161 triggering events, winter season) from biogas plant A and 6.80/7.44gCH4kWhel(-1) (3.60/3.88% CH4-loss, within 66days, 452 triggering events, summer season) from biogas plant B. Besides the operational state of the biogas plant (e.g. malfunction of the combined heat and power unit), the mode of operation of the biogas flare, which can be manually or automatically operated as well as the atmospheric conditions (e.g. drop of the atmospheric pressure) can also affect the biogas emission from PRVs. PMID:26944456

  7. Model-based design of an agricultural biogas plant: application of anaerobic digestion model no.1 for an improved four chamber scheme.

    PubMed

    Wett, B; Schoen, M; Phothilangka, P; Wackerle, F; Insam, H

    2007-01-01

    Different digestion technologies for various substrates are addressed by the generic process description of Anaerobic Digestion Model No. 1. In the case of manure or agricultural wastes a priori knowledge about the substrate in terms of ADM1 compounds is lacking and influent characterisation becomes a major issue. The actual project has been initiated for promotion of biogas technology in agriculture and for expansion of profitability also to rather small capacity systems. In order to avoid costly individual planning and installation of each facility a standardised design approach needs to be elaborated. This intention pleads for bio kinetic modelling as a systematic tool for process design and optimisation. Cofermentation under field conditions was observed, quality data and flow data were recorded and mass flow balances were calculated. In the laboratory different substrates have been digested separately in parallel under specified conditions. A configuration of four ADM1 model reactors was set up. Model calibration identified disintegration rate, decay rates for sugar degraders and half saturation constant for sugar as the three most sensitive parameters showing values (except the latter) about one order of magnitude higher than default parameters. Finally, the model is applied to the comparison of different reactor configurations and volume partitions. Another optimisation objective is robustness and load flexibility, i.e. the same configuration should be adaptive to different load situations only by a simple recycle control in order to establish a standardised design. PMID:17564366

  8. Comparative life cycle assessment of biogas plant configurations for a demand oriented biogas supply for flexible power generation.

    PubMed

    Hahn, Henning; Hartmann, Kilian; Bühle, Lutz; Wachendorf, Michael

    2015-03-01

    The environmental performance of biogas plant configurations for a demand - oriented biogas supply for flexible power generation is comparatively assessed in this study. Those configurations indicate an increased energy demand to operate the operational enhancements compared to conventional biogas plants supplying biogas for baseload power generation. However, findings show that in contrast to an alternative supply of power generators with natural gas, biogas supplied on demand by adapted biogas plant configurations saves greenhouse gas emissions by 54-65 g CO(2-eq) MJ(-1) and primary energy by about 1.17 MJ MJ(-1). In this regard, configurations with flexible biogas production profit from reduced biogas storage requirements and achieve higher savings compared to configurations with continuous biogas production. Using thicker biogas storage sheeting material reduces the methane permeability of up to 6m(3) d(-1) which equals a reduction of 8% of the configuration's total methane emissions. PMID:25553565

  9. Metaproteomics of complex microbial communities in biogas plants

    PubMed Central

    Heyer, Robert; Kohrs, Fabian; Reichl, Udo; Benndorf, Dirk

    2015-01-01

    Production of biogas from agricultural biomass or organic wastes is an important source of renewable energy. Although thousands of biogas plants (BGPs) are operating in Germany, there is still a significant potential to improve yields, e.g. from fibrous substrates. In addition, process stability should be optimized. Besides evaluating technical measures, improving our understanding of microbial communities involved into the biogas process is considered as key issue to achieve both goals. Microscopic and genetic approaches to analyse community composition provide valuable experimental data, but fail to detect presence of enzymes and overall metabolic activity of microbial communities. Therefore, metaproteomics can significantly contribute to elucidate critical steps in the conversion of biomass to methane as it delivers combined functional and phylogenetic data. Although metaproteomics analyses are challenged by sample impurities, sample complexity and redundant protein identification, and are still limited by the availability of genome sequences, recent studies have shown promising results. In the following, the workflow and potential pitfalls for metaproteomics of samples from full-scale BGP are discussed. In addition, the value of metaproteomics to contribute to the further advancement of microbial ecology is evaluated. Finally, synergistic effects expected when metaproteomics is combined with advanced imaging techniques, metagenomics, metatranscriptomics and metabolomics are addressed. PMID:25874383

  10. An integrated metagenome and -proteome analysis of the microbial community residing in a biogas production plant.

    PubMed

    Ortseifen, Vera; Stolze, Yvonne; Maus, Irena; Sczyrba, Alexander; Bremges, Andreas; Albaum, Stefan P; Jaenicke, Sebastian; Fracowiak, Jochen; Pühler, Alfred; Schlüter, Andreas

    2016-08-10

    To study the metaproteome of a biogas-producing microbial community, fermentation samples were taken from an agricultural biogas plant for microbial cell and protein extraction and corresponding metagenome analyses. Based on metagenome sequence data, taxonomic community profiling was performed to elucidate the composition of bacterial and archaeal sub-communities. The community's cytosolic metaproteome was represented in a 2D-PAGE approach. Metaproteome databases for protein identification were compiled based on the assembled metagenome sequence dataset for the biogas plant analyzed and non-corresponding biogas metagenomes. Protein identification results revealed that the corresponding biogas protein database facilitated the highest identification rate followed by other biogas-specific databases, whereas common public databases yielded insufficient identification rates. Proteins of the biogas microbiome identified as highly abundant were assigned to the pathways involved in methanogenesis, transport and carbon metabolism. Moreover, the integrated metagenome/-proteome approach enabled the examination of genetic-context information for genes encoding identified proteins by studying neighboring genes on the corresponding contig. Exemplarily, this approach led to the identification of a Methanoculleus sp. contig encoding 16 methanogenesis-related gene products, three of which were also detected as abundant proteins within the community's metaproteome. Thus, metagenome contigs provide additional information on the genetic environment of identified abundant proteins. PMID:27312700

  11. Consequential environmental life cycle assessment of a farm-scale biogas plant.

    PubMed

    Van Stappen, Florence; Mathot, Michaël; Decruyenaere, Virginie; Loriers, Astrid; Delcour, Alice; Planchon, Viviane; Goffart, Jean-Pierre; Stilmant, Didier

    2016-06-15

    Producing biogas via anaerobic digestion is a promising technology for meeting European and regional goals on energy production from renewable sources. It offers interesting opportunities for the agricultural sector, allowing waste and by-products to be converted into bioenergy and bio-based materials. A consequential life cycle assessment (cLCA) was conducted to examine the consequences of the installation of a farm-scale biogas plant, taking account of assumptions about processes displaced by biogas plant co-products (power, heat and digestate) and the uses of the biogas plant feedstock prior to plant installation. Inventory data were collected on an existing farm-scale biogas plant. The plant inputs are maize cultivated for energy, solid cattle manure and various by-products from surrounding agro-food industries. Based on hypotheses about displaced electricity production (oil or gas) and the initial uses of the plant feedstock (animal feed, compost or incineration), six scenarios were analyzed and compared. Digested feedstock previously used in animal feed was replaced with other feed ingredients in equivalent feed diets, designed to take account of various nutritional parameters for bovine feeding. The displaced production of mineral fertilizers and field emissions due to the use of digestate as organic fertilizer was balanced against the avoided use of manure and compost. For all of the envisaged scenarios, the installation of the biogas plant led to reduced impacts on water depletion and aquatic ecotoxicity (thanks mainly to the displaced mineral fertilizer production). However, with the additional animal feed ingredients required to replace digested feedstock in the bovine diets, extra agricultural land was needed in all scenarios. Field emissions from the digestate used as organic fertilizer also had a significant impact on acidification and eutrophication. The choice of displaced marginal technologies has a huge influence on the results, as have the

  12. Effects of biogas digestate on soil properties and plant growth

    NASA Astrophysics Data System (ADS)

    Gulyás, Miklós; Füleky, György

    2013-04-01

    Farming methods and food industries generate large amounts manure and other useful raw materials that need safe disposal. Following the international trends great numbers of biogas plants were opened during the last few years in Hungary. However this issue presents a number of new questions, including the subsequent use of anaerobic fermentation residues. So far we have only limited information about it's agricultural applications. Farmers and authorities are very skeptic because feedstocks are very different so the endproduct will be different, too. However, this endproduct can be applied as fertilizer. The aim of our work is to determine the effects of this product in plant-soil system. Digestate contains high amount of nitrogen which is present mainly ammonium form and this form can cause root depression and lower germination rates. Pot experiments were established with different rates of nitrogen content (80 kg ha-1N, 120 kg ha-1N, 170 kg ha-1N, and control). Maximum rates were determine by the Nitrate Directive. Soil moisture was 60% of maximum of water capacity. Digestate and distilled water were homogenized and added to 200g loamy soil. Rye-grass (Lolium perenne) was applied as a test plant. Treatments were randomized design and 10 replications. Three pot from each treatment were used to observe the germination and progress of plants. We investigated the effect of the digestate on nitrate- and ammonium-ion content of soil. The amount of nitrate- and ammonium-N of soil was determine with distillation. The ammonium-N levels increased with the doses on the first day but on the sixth-seventh day this amount totally falled down, because NH4-N transformed to NO3-N. Nitrate level increased continuously untill the tenth day, later decreased as the result of the plant and microbes consumption. The increasing doses inhibited the germination and root development of the plants. We experienced fewer roots, which were different form control.

  13. LED-Absorption-QEPAS Sensor for Biogas Plants

    PubMed Central

    Köhring, Michael; Böttger, Stefan; Willer, Ulrike; Schade, Wolfgang

    2015-01-01

    A new sensor for methane and carbon dioxide concentration measurements in biogas plants is presented. LEDs in the mid infrared spectral region are implemented as low cost light source. The combination of quartz-enhanced photoacoustic spectroscopy with an absorption path leads to a sensor setup suitable for the harsh application environment. The sensor system contains an electronics unit and the two gas sensors; it was designed to work as standalone device and was tested in a biogas plant for several weeks. Gas concentration dependent measurements show a precision better than 1% in a range between 40% and 60% target gas concentration for both sensors. Concentration dependent measurements with different background gases show a considerable decrease in cross sensitivity against the major components of biogas in direct comparison to common absorption based sensors. PMID:26007746

  14. Emergy analysis of a farm biogas project in China: A biophysical perspective of agricultural ecological engineering

    NASA Astrophysics Data System (ADS)

    Zhou, S. Y.; Zhang, B.; Cai, Z. F.

    2010-05-01

    This paper aims to present a biophysical understanding of the agricultural ecological engineering by emergy analysis for a farm biogas project in China as a representative case. Accounting for the resource inputs into and accumulation within the project, as well as the outputs to the social system, emergy analysis provides an empirical study in the biophysical dimension of the agricultural ecological engineering. Economic benefits and ecological economic benefits of the farm biogas project indicated by market value and emergy monetary value are discussed, respectively. Relative emergy-based indices such as renewability (R%), emergy yield ratio (EYR), environmental load ratio (ELR) and environmental sustainability index (ESI) are calculated to evaluate the environmental load and local sustainability of the concerned biogas project. The results show that the farm biogas project has more reliance on the local renewable resources input, less environmental pressure and higher sustainability compared with other typical agricultural systems. In addition, holistic evaluation and its policy implications for better operation and management of the biogas project are presented.

  15. Detection of pathogenic clostridia in biogas plant wastes.

    PubMed

    Neuhaus, Jürgen; Shehata, Awad A; Krüger, Monika

    2015-01-01

    As the number of biogas plants has grown rapidly in the last decade, the amount of potentially contaminated wastes with pathogenic Clostridium spp. has increased as well. This study reports the results from examining 203 biogas plant wastes (BGWs). The following Clostridium spp. with different frequencies could be isolated via a new enrichment medium (Krüne medium) and detected by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS): Clostridium perfringens (58 %) then Clostridium bifermentans (27 %), Clostridium tertium (23 %) and Clostridium butyricum (19 %), Clostridium cadaveris (15 %), Clostridium parapurificum (6 %), Clostridium glycolicum (5 %), Clostridium baratii (4 %), Clostridium sporogenes (2 %), Clostridium sordellii (1 %) and Clostridium subterminale (0.5 %). The mean most probable number (MPN) count of sulfite reducing bacteria was between 10(3) and 10(4)/mL, and the higher the MPN, the more pathogenic Clostridium spp. were present. Also, real-time PCR was used to be compared with culture method for C. perfringens, C. bifermentans, C. butyricum, C. sporogenes/Clostridium botulinum and C. sordellii. Although real-time PCR was more sensitive than the culture method, both systems improve the recovery rate but in different ways and are useful to determine pathogenic clostridia in biogas plants. In conclusion, BGWs could present a biohazard risk of clostridia for humans and animals. PMID:24984829

  16. Hazardous organic compounds in biogas plant end products--soil burden and risk to food safety.

    PubMed

    Suominen, K; Verta, M; Marttinen, S

    2014-09-01

    The end products (digestate, solid fraction of the digestate, liquid fraction of the digestate) of ten biogas production lines in Finland were analyzed for ten hazardous organic compounds or compound groups: polychlorinated dibenzo-p-dioxins and furans (PCDD/Fs), polychlorinated biphenyls (PCB(7)), polyaromatic hydrocarbons (PAH(16)), bis-(2-ethylhexyl) phthalate (DEHP), perfluorinated alkyl compounds (PFCs), linear alkylbenzene sulfonates (LASs), nonylphenols and nonylphenol ethoxylates (NP+NPEOs), polybrominated diphenyl ethers (PBDEs), hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA). Biogas plant feedstocks were divided into six groups: municipal sewage sludge, municipal biowaste, fat, food industry by-products, animal manure and others (consisting of milling by-products (husk) and raw former foodstuffs of animal origin from the retail trade). There was no clear connection between the origin of the feedstocks of a plant and the concentrations of hazardous organic compounds in the digestate. For PCDD/Fs and for DEHP, the median soil burden of the compound after a single addition of digestate was similar to the annual atmospheric deposition of the compound or compound group in Finland or other Nordic countries. For PFCs, the median soil burden was somewhat lower than the atmospheric deposition in Finland or Sweden. For NP+NPEOs, the soil burden was somewhat higher than the atmospheric deposition in Denmark. The median soil burden of PBDEs was 400 to 1000 times higher than the PBDE air deposition in Finland or in Sweden. With PBDEs, PFCs and HBCD, the impact of the use of end products should be a focus of further research. Highly persistent compounds, such as PBDE- and PFC-compounds may accumulate in agricultural soil after repeated use of organic fertilizers containing these compounds. For other compounds included in this study, agricultural use of biogas plant end products is unlikely to cause risk to food safety in Finland. PMID:24593894

  17. Ecotoxicological assessment of residues from different biogas production plants used as fertilizer for soil.

    PubMed

    Stefaniuk, Magdalena; Bartmiński, Piotr; Różyło, Krzysztof; Dębicki, Ryszard; Oleszczuk, Patryk

    2015-11-15

    Residues from biogas production (RBP) are a relatively new materials, which may be an interesting resource for the improvement of soil fertility. Nevertheless, in spite of the potential benefits from the agricultural utilization of RBP, there is a need of comprehensive estimation of their toxicity. This information is needed to exclude potential negative environmental impacts arising from the use of RBP. Samples of RBP obtained from six biogas production plants with varied biogas production methods were analysed. The samples with and without separation on solid and liquid phases were investigated. The physicochemical properties of the RBP, heavy metals content (Cr, Cu, Ni, Cd, Pb i Zn) and toxicity on bacteria (Vibrio fischeri, MARA test - 11 different strains), collembolans (Folsomia candida) and two plant species (Lepidium sativum and Sinapis alba) was investigated. Toxicity of RBP was examined using Phytotoxkit F (root growth inhibition), collembolan test (mortality, inhibition of reproduction), Microtox® (inhibition of the luminescence of V. fischeri) and MARA test (growth of microorganisms). An especially negative effect on the tested organisms whereas was noted for the liquid phase after separation. In many cases, RBP without separation also showed unfavourable effects on the tested organisms. Liquid phase after separation and non-separated materials caused inhibition of root growth of L. sativum and S. alba at the level of 17.42-100% and 30.5-100%, respectively, as well as the inhibition of reproduction of F. candida with the range from 68.89 to 100%. In most cases, no ecotoxicological effect was observed for solid phase after separation for tested organisms. The solid phase after separation presented the most favorable properties between all investigated RBP. Therefore, it can be a potential material for the improvement of soil properties and for later use in agriculture. PMID:26057443

  18. Electricity from biogas

    SciTech Connect

    Augenstein, D.; Benemann, J.; Hughes, E.

    1994-12-31

    Biogas is a medium-Btu methane and carbon dioxide mix produced by bacterial decomposition of organic matter. Its sources include landfills, waste water sludges, and animal wastes. It can fuel energy applications, of which electricity generation is a frequently-preferred option. The greatest current U.S. biogas recovery and energy use is at landfills, where biogas at about 80 landfill sites fuels a total of approximately 300 MWe. Wastewater treatment plants and confined animal waste management systems support additional electric power production. Generation of electricity from biogas can present difficulties due to the generally small scale of the generating facility, variable energy content of the gas, fluctuating availability, contaminant problems, and often-demanding control needs. However, such difficulties are being successfully addressed and economics for electricity generation are often favorable as biogas can be essentially {open_quotes}free{close_quotes} fuel. Biogas recovery and use has the additional advantage of mitigating a potent greenhouse gas. Biogas from U.S. landfills alone could fuel about 1% of U.S. electrical generation while giving climate change benefit equivalent to reducing CO{sub 2} emissions in the electricity sector by more than 10%. Growth in landfill gas use will be facilitated by recent regulations, advances in equipment, and improved management techniques such as {open_quotes}controlled landfilling{close_quotes}. The potential for biogas recovery and electricity production from sewage sludges, animal wastes and other organic resources such as agricultural residues is uncertain but probably exceeds the estimate for landfills.

  19. Ecological analysis of a typical farm-scale biogas plant in China

    NASA Astrophysics Data System (ADS)

    Duan, Na; Lin, Cong; Wang, Pingzhi; Meng, Jing; Chen, Hui; Li, Xue

    2014-09-01

    The aim of this work was to present the common anaerobic digestion technologies in a typical farm-scale biogas plant in China. The comprehensive benefits of most biogas plants in China have not been fully assessed in past decades due to the limited information of the anaerobic digestion processes in biogas plants. This paper analyzed four key aspects (i.e., operational performance, nonrenewable energy (NE) savings, CO2 emission reduction (CER) and economic benefits (EBs)) of a typical farm-scale biogas plant, where beef cattle manure was used as feedstock. Owing to the monitoring system, stable operation was achieved with a hydraulic retention time of 18-22 days and a production of 876,000 m3 of biogas and 37,960 t of digestate fertilizer annually. This could substantially substitute for the nonrenewable energy and chemical fertilizer. The total amount of NE savings and CER derived from biogas and digestate fertilizer was 2.10×107 MJ (equivalent to 749.7 tce) and 9.71×105 kg, respectively. The EBs of the biogas plant was 6.84×105 CNY·yr-1 with an outputs-to-inputs ratio of 2.37. As a result, the monitoring system was proved to contribute significantly to the sound management and quantitative assessment of the biogas plant. Biogas plants could produce biogas which could be used to substitute fossil fuels and reduce the emissions of greenhouse gases, and digestate fertilizer is also an important bio-product.

  20. Innovative test method for the estimation of the foaming tendency of substrates for biogas plants

    SciTech Connect

    Moeller, Lucie; Eismann, Frank; Wißmann, Daniel; Nägele, Hans-Joachim; Zielonka, Simon; Müller, Roland A.; Zehnsdorf, Andreas

    2015-07-15

    Graphical abstract: Display Omitted - Highlights: • Foaming in biogas plants depends on the interactions between substrate and digestate. • Foaming tests enable the evaluation of substrate foaming tendency in biogas plants. • Leipzig foam tester enables foaming tests of substrates prior to use. - Abstract: Excessive foaming in anaerobic digestion occurs at many biogas plants and can cause problems including plugged gas pipes. Unfortunately, the majority of biogas plant operators are unable to identify the causes of foaming in their biogas reactor. The occurrence of foaming is often related to the chemical composition of substrates fed to the reactor. The consistency of the digestate itself is also a crucial part of the foam formation process. Thus, no specific recommendations concerning substrates can be given in order to prevent foam formation in biogas plants. The safest way to avoid foaming is to test the foaming tendency of substrates on-site. A possible solution is offered by an innovative foaming test. With the help of this tool, biogas plant operators can evaluate the foaming disposition of new substrates prior to use in order to adjust the composition of substrate mixes.

  1. Determination of methane emission rates on a biogas plant using data from laser absorption spectrometry.

    PubMed

    Groth, Angela; Maurer, Claudia; Reiser, Martin; Kranert, Martin

    2015-02-01

    The aim of the work was to establish a method for emission control of biogas plants especially the observation of fugitive methane emissions. The used method is in a developmental stage but the topic is crucial to environmental and economic issues. A remote sensing measurement method was adopted to determine methane emission rates of a biogas plant in Rhineland-Palatinate, Germany. An inverse dispersion model was used to deduce emission rates. This technique required one concentration measurement with an open path tunable diode laser absorption spectrometer (TDLAS) downwind and upwind the source and basic wind information, like wind speed and direction. Different operating conditions of the biogas plant occurring on the measuring day (December 2013) could be represented roughly in the results. During undisturbed operational modes the methane emission rate averaged 2.8 g/s, which corresponds to 4% of the methane gas production rate of the biogas plant. PMID:25446786

  2. Theoretical and experimental investigations of thermal conditions of household biogas plant

    NASA Astrophysics Data System (ADS)

    Zhelykh, Vasil; Furdas, Yura; Dzeryn, Oleksandra

    2016-06-01

    The construction of domestic continuous bioreactor is proposed. The modeling of thermal modes of household biogas plant using graph theory was done. The correction factor taking into account with the influence of variables on its value was determined. The system of balance equations for the desired thermal conditions in the bioreactor was presented. The graphical and analytical capabilities were represented that can be applied in the design of domestic biogas plants of organic waste recycling.

  3. Biogas production from plant biomass used for phytoremediation of industrial wastes.

    PubMed

    Verma, V K; Singh, Y P; Rai, J P N

    2007-05-01

    In present study, potentials of water hyacinth (Eichhornia crassipes) and water chestnut (Trapa bispinnosa) employed for phytoremediation of toxic metal rich brass and electroplating industry effluent, were examined in terms of biogas generation. Inability of the plants to grow in undiluted effluent directed to select 20%, 40% and 60% effluent concentrations (with deionized water) for phytoremediation experiments. Slurry of both the plants used for phytoremediation produced significantly more biogas than that by the control plants grown in unpolluted water; the effect being more pronounced with plants used for phytoremediation of 20% effluent. Maximum cumulative production of biogas (2430c.c./100gdm of water hyacinth and 1940c.c./100gdm of water chest nut) and per cent methane content (63.82% for water hyacinth and 57.04% for water chestnut) was observed at 5mm particle size and 1:1 substrate/inoculum ratio, after twenty days incubation. Biogas production was quicker (maximum from 8-12days) in water hyacinth than in water chestnut (maximum from 12-16days). The qualitative and quantitative variations in biogas production were correlated with COD, C, N, C/N ratio and toxic metal contents of the slurry used. PMID:16831546

  4. Influence of DNA isolation method on the investigation of archaeal diversity and abundance in biogas plants.

    PubMed

    Theiss, Juliane; Rother, Michael; Röske, Kerstin

    2016-09-01

    Various methods are available for DNA isolation from environmental samples. Because the chemical and biological composition of samples such as soil, sludge, or plant material is different, the effectiveness of DNA isolation can vary depending on the method applied and thus, have a substantial effect on the results of downstream analysis of the microbial community. Although the process of biogas formation is being intensely investigated, a systematic evaluation of kits for DNA isolation from material of biogas plants is still lacking. Since no DNA isolation kit specifically tailored for DNA isolation from sludge of biogas plants is available, this study compares five commercially available kits regarding their influence on downstream analyses such denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR (qPCR). The results show that not all kits are equally suited for the DNA isolation from samples of different biogas plants, but highly reproducible DGGE fingerprints as well as qPCR results across the tested samples from biogas reactors using different substrate compositions could be produced using selected kits. PMID:27089887

  5. Methane emission from fixed dome biogas plants in hilly and plain regions of northern India.

    PubMed

    Khoiyangbam, R S; Kumar, Sushil; Jain, M C; Gupta, Navindu; Kumar, Arun; Kumar, Vinod

    2004-10-01

    Methane emissions from the slurry displacement chambers of different fixed dome type biogas plants (Capacity 2 m3) installed in hilly and plain regions of northern India were quantitatively estimated. The slurry temperature in the biogas plants in the hilly areas of Himachal Pradesh at an altitude of 1300 m above mean sea level remains below the lower mesophilic range (16-25 degrees C) during most part of the year. Difference in the ambient temperature under the two climatic conditions of Hills and plain regions affects the CH4 flux. Methane fluxes from the plants ranged between 7 and 120 gm(-2) d(-1). In northern plain, temperature remains warm (21-33 degrees C) throughout the year except during winter (December to January) when there is a steep fall. Seasonal emission of CH4 ranged between 10 and 178 gm(-2) d(-1). The annual average methane emission from the biogas plants in plain areas was 83.1 gm(-2) d(-1) as compared to 43.1 gm(-2) d(-1)in the hilly areas. Annual contribution per plant to the global methane budget from a fixed dome biogas plant (Cap 2 m3) in plain region of northern India was 53.2 kg as compared 22.3 kg in hilly area. PMID:15207292

  6. Biogas: the Chinese experience

    SciTech Connect

    Thorhaug, A.

    1980-10-01

    This article details the history of biogas in China and its development as a renewable energy source. There are 7.2 million biogas plants that benefit directly 35 million rural people. The average family digester costs $20 to $40 and can easily be made by unskilled technical workers. The design of a typical digester is described together with its maintenance and recovery of gas. The uses of biogas in China are numerous and solves most of the major energy problems of rural life such as village cooking, and fuel for irrigation pumps and farm tractors. The utilizing of human, animal and agricultural waste is in itself a large benefit and a list of other benefits to the Chinese is given.

  7. Detection of Clostridium botulinum in liquid manure and biogas plant wastes.

    PubMed

    Neuhaus, Jürgen; Schrödl, Wieland; Shehata, Awad A; Krüger, Monika

    2015-09-01

    Biogas plants have been considered as a source for possible amplification and distribution of pathogenic bacteria capable of causing severe infections in humans and animals. Manure and biogas wastes could be sources for spore-forming bacteria such as Clostridium botulinum. In the present study, 24 liquid manure and 84 biogas waste samples from dairies where the majority of the cows suffered from chronic botulism were investigated for the presence of botulinum neurotoxins (BoNT) and C. botulinum spores. The prevalence of BoNT/A, B, C, D, and E in biogas wastes was 16.6, 8.3, 10.7, 7.1, and 10.8 %, respectively, while in manure, the prevalence was 0.0, 0.0, 0.0, 8.3, and 4.1 %, respectively. After enrichment of samples in reinforced cultural medium, they were tested for C. botulinum BoNT/A, B, C, D, and E using ELISA (indirect C. botulinum detection). The prevalence of C. botulinum type A, B, C, D, and E samples in biogas wastes was 20.2, 15.5, 19, 10.7, and 34.8 %, respectively, while the prevalence in liquid manure was 0.0, 0.0, 0.0, 8.3, and 12.5 %, respectively. In conclusion, the occurrence of BoNT and C. botulinum spores in biogas waste of diseased animals indicates an increased and underestimated hygienic risk. Application of digestates from biogas fermentations as fertilizers could lead to an accumulation of long lifespan spores in the environment and could be a possible health hazard. PMID:25753763

  8. The effect of electron acceptors on biogas production from tannery sludge of a Mexican wastewater plant

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Effluents from the leather processing plants generally are discharged into rivers or are used to irrigate farmland. The biogas production from the digestion of sludge produced could be used as alternative sources for energy and power generation. A study was carried out to examine the effects of vari...

  9. The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology.

    PubMed

    Schlüter, Andreas; Bekel, Thomas; Diaz, Naryttza N; Dondrup, Michael; Eichenlaub, Rudolf; Gartemann, Karl-Heinz; Krahn, Irene; Krause, Lutz; Krömeke, Holger; Kruse, Olaf; Mussgnug, Jan H; Neuweger, Heiko; Niehaus, Karsten; Pühler, Alfred; Runte, Kai J; Szczepanowski, Rafael; Tauch, Andreas; Tilker, Alexandra; Viehöver, Prisca; Goesmann, Alexander

    2008-08-31

    Composition and gene content of a biogas-producing microbial community from a production-scale biogas plant fed with renewable primary products was analysed by means of a metagenomic approach applying the ultrafast 454-pyrosequencing technology. Sequencing of isolated total community DNA on a Genome Sequencer FLX System resulted in 616,072 reads with an average read length of 230 bases accounting for 141,664,289 bases sequence information. Assignment of obtained single reads to COG (Clusters of Orthologous Groups of proteins) categories revealed a genetic profile characteristic for an anaerobic microbial consortium conducting fermentative metabolic pathways. Assembly of single reads resulted in the formation of 8752 contigs larger than 500 bases in size. Contigs longer than 10kb mainly encode house-keeping proteins, e.g. DNA polymerase, recombinase, DNA ligase, sigma factor RpoD and genes involved in sugar and amino acid metabolism. A significant portion of contigs was allocated to the genome sequence of the archaeal methanogen Methanoculleus marisnigri JR1. Mapping of single reads to the M. marisnigri JR1 genome revealed that approximately 64% of the reference genome including methanogenesis gene regions are deeply covered. These results suggest that species related to those of the genus Methanoculleus play a dominant role in methanogenesis in the analysed fermentation sample. Moreover, assignment of numerous contig sequences to clostridial genomes including gene regions for cellulolytic functions indicates that clostridia are important for hydrolysis of cellulosic plant biomass in the biogas fermenter under study. Metagenome sequence data from a biogas-producing microbial community residing in a fermenter of a biogas plant provide the basis for a rational approach to improve the biotechnological process of biogas production. PMID:18597880

  10. Detailed monitoring of two biogas plants and mechanical solid-liquid separation of fermentation residues.

    PubMed

    Bauer, Alexander; Mayr, Herwig; Hopfner-Sixt, Katharina; Amon, Thomas

    2009-06-01

    The Austrian "green electricity act" (Okostromgesetz) has led to an increase in biogas power plant size and consequently to an increased use of biomass. A biogas power plant with a generating capacity of 500 kW(el) consumes up to 38,000 kg of biomass per day. 260 ha of cropland is required to produce this mass. The high water content of biomass necessitates a high transport volume for energy crops and fermentation residues. The transport and application of fermentation residues to farmland is the last step in this logistic chain. The use of fermentation residues as fertilizer closes the nutrient cycle and is a central element in the efficient use of biomass for power production. Treatment of fermentation residues by separation into liquid and solid phases may be a solution to the transport problem. This paper presents detailed results from the monitoring of two biogas plants and from the analysis of the separation of fermentation residues. Furthermore, two different separator technologies for the separation of fermentation residues of biogas plants were analyzed. The examined biogas plants correspond to the current technological state of the art and have designs developed specifically for the utilization of energy crops. The hydraulic retention time ranged between 45.0 and 83.7 days. The specific methane yields were 0.40-0.43 m(3)N CH(4) per kg VS. The volume loads ranged between 3.69 and 4.00 kg VS/m(3). The degree of degradation was between 77.3% and 82.14%. The screw extractor separator was better suited for biogas slurry separation than the rotary screen separator. The screw extractor separator exhibited a high throughput and good separation efficiency. The efficiency of slurry separation depended on the dry matter content of the fermentation residue. The higher the dry matter content, the higher the proportion of solid phase after separation. In this project, we found that the fermentation residues could be divided into 79.2% fluid phase with a dry matter

  11. Estimation of potential biomass resource and biogas production from aquatic plants in Argentina

    NASA Astrophysics Data System (ADS)

    Fitzsimons, R. E.; Laurino, C. N.; Vallejos, R. H.

    1982-08-01

    The use of aquatic plants in artificial lakes as a biomass source for biogas and fertilizer production through anaerobic fermentation is evaluated, and the magnitude of this resource and the potential production of biogas and fertilizer are estimated. The specific case considered is the artificial lake that will be created by the construction of Parana Medio Hydroelectric Project on the middle Parana River in Argentina. The growth of the main aquatic plant, water hyacinth, on the middle Parana River has been measured, and its conversion to methane by anaerobic fermentation is determined. It is estimated that gross methane production may be between 1.0-4.1 x 10 to the 9th cu cm/year. The fermentation residue can be used as a soil conditioner, and it is estimated production of the residue may represent between 54,900-221,400 tons of nitrogen/year, a value which is 2-8 times the present nitrogen fertilizer demand in Argentina.

  12. Biogas production from coumarin-rich plants--inhibition by coumarin and recovery by adaptation of the bacterial community.

    PubMed

    Popp, Denny; Schrader, Steffi; Kleinsteuber, Sabine; Harms, Hauke; Sträuber, Heike

    2015-09-01

    Plants like sweet clover (Melilotus spp.) are not suitable as fodder for cattle because of harmful effects of the plant secondary metabolite coumarin. As an alternative usage, the applicability of coumarin-rich plants as substrates for biogas production was investigated. When coumarin was added to continuous fermentation processes codigesting grass silage and cow manure, it caused a strong inhibition noticeable as decrease of biogas production by 19% and increase of metabolite concentrations to an organic acids/alkalinity ratio higher than 0.3(gorganic acids) gCaCO3 (-1). Microbial communities of methanogenic archaea were dominated by the genera Methanosarcina (77%) and Methanoculleus (11%). This community composition was not influenced by coumarin addition. The bacterial community analysis unraveled a divergence caused by coumarin addition correlating with the anaerobic degradation of coumarin and the recovery of the biogas process. As a consequence, biogas production resumed similar to the coumarin-free control with a biogas yield of 0.34 LN g(volatile solids) (-1) and at initial metabolite concentrations (∼ 0.2 g(organic acids) gCaCO3 (-1)). Coumarin acts as inhibitor and as substrate during anaerobic digestion. Hence, coumarin-rich plants might be suitable for biogas production, but should only be used after adaptation of the microbial community to coumarin. PMID:26324856

  13. Conceptual design of an integrated hydrothermal liquefaction and biogas plant for sustainable bioenergy production.

    PubMed

    Hoffmann, Jessica; Rudra, Souman; Toor, Saqib S; Holm-Nielsen, Jens Bo; Rosendahl, Lasse A

    2013-02-01

    Initial process studies carried out in Aspen Plus on an integrated thermochemical conversion process are presented herein. In the simulations, a hydrothermal liquefaction (HTL) plant is combined with a biogas plant (BP), such that the digestate from the BP is converted to a biocrude in the HTL process. This biorefinery concept offers a sophisticated and sustainable way of converting organic residuals into a range of high-value biofuel streams in addition to combined heat and power (CHP) production. The primary goal of this study is to provide an initial estimate of the feasibility of such a process. By adding a diesel-quality-fuel output to the process, the product value is increased significantly compared to a conventional BP. An input of 1000 kg h(-1) manure delivers approximately 30-38 kg h(-1) fuel and 38-61 kg h(-1) biogas. The biogas can be used to upgrade the biocrude, to supply the gas grid or for CHP. An estimated 62-84% of the biomass energy can be recovered in the biofuels. PMID:23262018

  14. Determination of gas composition in a biogas plant using a Raman-based sensor system

    NASA Astrophysics Data System (ADS)

    Eichmann, S. C.; Kiefer, J.; Benz, J.; Kempf, T.; Leipertz, A.; Seeger, T.

    2014-07-01

    We propose a gas sensor, based on spontaneous Raman scattering, for the compositional analysis of typical biogas mixtures and present a description of the sensor, as well as of the calibration procedure, which allows the quantification of condensable gases. Moreover, we carry out a comprehensive characterization of the system, in order to determine the measurement uncertainty, as well as influences of temperature and pressure fluctuation. Finally, the sensor is applied at different locations inside a plant in which biogas is produced from renewable raw materials. The composition is monitored after fermenting, after purification and after the final conditioning, where natural gas is added. The Raman sensor is able to detect all the relevant gas components, i.e. CH4, CO2, N2 and H2O, and report their individual concentrations over time. The results were compared to reference data from a conventional gas analyzer and good agreement was obtained.

  15. A review on palm oil mill biogas plant wastewater treatment using coagulation-ozonation

    NASA Astrophysics Data System (ADS)

    Dexter, Z. D.; Joseph, C. G.; Zahrim, A. Y.

    2016-06-01

    Palm oil mill effluent (POME) generated from the palm oil industry is highly polluted and requires urgent attention for treatment due to its high organic content. Biogas plant containing anaerobic digester is capable to treat the high organic content of the POME while generating valuable biogas at the same time. This green energy from POME is environmental-friendly but the wastewater produced is still highly polluted and blackish in colour. Therefore a novel concept of combining coagulation with ozonation treatment is proposed to treat pollution of this nature. Several parameters should be taken under consideration in order to ensure the effectiveness of the hybrid treatment including ozone dosage, ozone contact time, pH of the water or wastewater, coagulant dosage, and mixing and settling time. This review paper will elucidate the importance of hybrid coagulation-ozonation treatment in producing a clear treated wastewater which is known as the main challenge in palm oil industry

  16. Plant ID. Agricultural Lesson Plans.

    ERIC Educational Resources Information Center

    Southern Illinois Univ., Carbondale. Dept. of Agricultural Education and Mechanization.

    This lesson plan is intended for use in conducting classes on plant identification. Presented first are a series of questions and answers designed to convey general information about the scientific classification of plants. The following topics are among those discussed: main types of plants; categories of vascular plants; gymnosperms and…

  17. Plant Tissues. Agricultural Lesson Plans.

    ERIC Educational Resources Information Center

    Southern Illinois Univ., Carbondale. Dept. of Agricultural Education and Mechanization.

    This lesson plan is intended for use in conducting classes on plant tissues. Presented first are an attention step and a series of questions and answers designed to convey general information about plant tissues and the effect of water and minerals on them. The following topics are among those discussed: reasons why water is important to plants,…

  18. Commercial Pesticides Applicator Manual: Agriculture - Plant.

    ERIC Educational Resources Information Center

    Fitzwater, W. D.; And Others

    This training manual provides information needed to meet the minimum EPA standards for certification as a commercial applicator of pesticides in the agriculture-plant pest control category. The text discusses identification and control of insects, diseases, nematodes, and weeds of agricultural crops. Proper use of application equipment and safety…

  19. Environmental assessment and finding of no significant impact: Biorecycling Technologies, Inc., Noble Biogas and Fertilizer Plant, Fresno County, California

    SciTech Connect

    1997-09-01

    The US Department of Energy (DOE) is considering a proposal from the California Energy Commission for partial funding up to $1,500,000 of the construction of the biorecycling Technologies, Inc., (BTI) Noble Biogas and Fertilizer Plant in Fresno County, California. BTI along with its contractors and business partners would develop the plant, which would use manure and green waste to produce biogas and a variety of organic fertilizer products. The California Energy Commission has requested funding from the DOE Commercialization Ventures program to assist in the construction of the plant, which would produce up to one megawatt of electricity by burning biogas in a cogeneration unit. The purpose of this environmental assessment (EA) is to provide DOE and the public with information on potential environmental impacts associated with funding development of the proposed project.

  20. Economic viability of biogas technology

    SciTech Connect

    Agrawal, S.C.; Agrawal, S.; Khare, O.P.

    1983-12-01

    Biogas has emerged as a suitable technology for providing alternative and additional sources of energy, especially for rural areas of developing countries. Although the experience gained in China and India established its technological success, social scientists are still involved in the controversial issue of its economic viability. The available literature presents very contradictory situations, ranging between the two extreme poles of high economic viability and nonviability. Such contradictory conclusions are derived since economic benefits from the technology are influenced by a number of factors. A review of the literature reveals that various factors are either not considered, or that the economics have been worked out assuming a very ideal situation, while biogas plants are operating under very different conditions. Using the coal replacement method even as coal is seldom used by villages is only a single example of this approach. In most of the developing countries, rural populations depend mainly on non-commercial fuels like firewood, dungcakes, agricultural wastes and leaves for cooking and heating purposes. Under the present technological limitations, biogas can most commonly be used for cooking and lighting. For testing the economic viability of biogas systems, a number of authors have considered the benefits in terms of savings in traditional fuels. But considering the actual thermal efficiency of different non-commercial fuel items, as well as biogas, it has still be be decided at what point of the market prices of fuel items that the biogas system becomes economically viable and remains viable. The present paper thus reviews different approaches adopted and suggested for working out the economics or the cost-benefit ratio of the biogas technology at the first stage, and then spells out the factors influencing the economic benefits of the technology under various situations, with the help of empirical

  1. Biogas - the calculable energy

    NASA Astrophysics Data System (ADS)

    Kith, Károly; Nagy, Orsolya; Balla, Zoltán; Tamás, András

    2015-04-01

    EU actions against climate change are rising energy prices, both have emphasized the use of renewable energy,increase investments and energy efficiency. A number of objectives formulated in the EC decree no. 29/2009 by 2020. This document is based on the share of renewable energies in energy consumption should be increased to 20% (EC, 2009). The EU average is 20% but the share of renewables vary from one member state to another. In Hungary in 2020, 14.65% renewable energy share is planned to be achieved. According to the latest Eurostat data, the share of renewable energy in energy consumption of the EU average was 14.1%, while in Hungary, this share was 9.6% in 2012. (EUROSTAT, 2014). The use of renewable energy plant level is influenced by several factors. The most important of these is the cost savings and efficiency gains. Hungarian investments in renewable energy production usually have high associated costs and the payback period is substantially more than five years, depending on the support rate. For example, the payback period is also influenced by the green electricity generated feed prices, which is one of the lowest in Hungary compared the Member States of the European Union. Consequently, it is important to increase the production of green energy. Nowadays, predictable biogas energy is an outstanding type of decentralized energy production. It follows directly that agricultural by-products can be used to produce energy and they also create jobs by the construction of a biogas plant. It is important to dispose of and destroy hazardous and noxious substances in energy production. It follows from this that the construction of biogas plants have a positive impact, in addition to green energy which is prepared to reduce the load on the environment. The production of biogas and green electricity is one of the most environment friendly forms of energy production. Biogas production also has other important ecological effects, such as the substitution of

  2. Updated guidebook on biogas development

    SciTech Connect

    Not Available

    1985-01-01

    On the practical aspects of small-scale biogas development suitable for use in rural areas of developing countries reviews biogas development throughout the ESCAP region and examines each of the steps involved in developing and operating a biogas plant. It details both the process and the microbiology of biogas fermentation and analyses the factors affecting gas plant design and operation. Also covered are the classification and design principles of plants, design, site, and site selection; starting and operating a gas plant; servicing and safety; and efficient plant performance. Also considered are the commercial uses of biogas and possible use of effluent.

  3. Isolation and characterization of microalgae for biodiesel production from Nisargruna biogas plant effluent.

    PubMed

    Tale, Manisha; Ghosh, Sukhendu; Kapadnis, Balasaheb; Kale, Sharad

    2014-10-01

    Increasing energy demand and depleting fossil fuel sources have intensified the focus on biofuel production. Microalgae have emerged as a desirable source for biofuel production because of high biomass and lipid production from waste water source. In this study, five microalgae were isolated from effluents of Nisargruna biogas plants. These isolates were identified based on morphology and partial 18S and 23S rRNA gene sequences. Growth and lipid accumulation potential of these microalgae were investigated. One isolate, Chlorella sp. KMN3, accumulated high biomass (1.59 ± 0.05 g L(-1)) with moderate lipid content (20%), while another isolate Monoraphidium sp. KMN5 showed moderate biomass accumulation of 0.65 ± 0.05 g L(-1) with a very high (35%) lipid content. The fatty acid methyl esters mainly composed of C-16:0, C-18:0, C-18:1 and C-18:2. This observation makes these microalgae immensely potential candidate for biodiesel production using the effluent of a biogas plant as feed stock. PMID:25063975

  4. Improving biogas quality and methane yield via co-digestion of agricultural and urban biomass wastes.

    PubMed

    Poulsen, Tjalfe G; Adelard, Laetitia

    2016-08-01

    Impact of co-digestion versus mono-digestion on biogas and CH4 yield for a set of five biomass materials (vegetable food waste, cow dung, pig manure, grass clippings, and chicken manure) was investigated considering 95 different biomass mixes of the five materials under thermophilic conditions in bench-scale batch experiments over a period of 65days. Average biogas and CH4 yields were significantly higher during co-digestion than during mono-digestion of the same materials. This improvement was most significant for co-digestion experiments involving three biomass types, although it was independent of the specific biomasses being co-digested. Improvement in CH4 production was further more prominent early in the digestion process during co-digestion compared to mono-digestion. Co-digestion also appeared to increase the ultimate CH4/CO2 ratio of the gas produced compared to mono-digestion although this tendency was relatively weak and not statistically significant. PMID:27256782

  5. Determining methane emissions from biogas plants--Operational and meteorological aspects.

    PubMed

    Hrad, Marlies; Piringer, Martin; Huber-Humer, Marion

    2015-09-01

    A micrometeorological method, combining an inverse dispersion technique with path-integrated concentration measurements, was applied on an Austrian biogas plant over the period of more than one year to determine emissions of the whole plant. Measurement campaigns were conducted to characterize the emission response to operational activities (e.g. digestate management) and meteorological changes. When digestate storage tanks were filled, an average emission rate of 7.2 kg CH4/h (approx. 4% of the calculated CH4 production) was determined, while 5.4 kg CH4/h of emissions (approx. 3% of the calculated CH4 production) were quantified after the tanks had been emptied. It could be observed that besides the operation mode (e.g. filling level or agitation of the openly stored digestate, maintenance), the meteorological conditions such as wind speed and solar radiation (e.g. heat flux) can also affect the emission rate. PMID:26000833

  6. Mapping of methane spatial distribution around biogas plant with an open-path tunable diode absorption spectroscopy scanning system

    NASA Astrophysics Data System (ADS)

    Zhang, Shirui; Wang, Jihua; Dong, Daming; Zheng, Wengang; Zhao, Xiande

    2013-02-01

    An open-path tunable diode laser absorption spectroscopy (OP-TDLAS) detector was applied to detect the methane emission from a biogas plant in a dairy farm. Two OP-TDLAS scanning systems were built according to maximum likelihood with expectation minimization (MLEM) and smooth basis function minimization (SBFM) algorithms to reconstruct the two-dimensional (2-D) distribution maps. Six reconstruction maps with the resolution of 30×80 were obtained by the MLEM algorithm with "grid translation method" and three reconstruction maps were obtained by the SBFM algorithm with 2-D Gaussian model. The maximum mixing ratio in the first result was between 0.85 and 1.30 ppm, while it was between 1.14 and 1.30 ppm in the second result. The average mixing ratio in the first result was between 0.54 and 0.49 ppm, and between 0.56 and 0.65 ppm in the second result. The reconstruction results validated that the two algorithms could effectively reflect the methane mixing ratio distribution within the target area. However, with the more simple optical rays and less equipment requirements, the OP-TDLAS scanning system based on SBFM algorithm provides a useful monitoring tool of methane emissions in agricultural production.

  7. Determination of volatile organic compounds from biowaste and co-fermentation biogas plants by single-sorbent adsorption.

    PubMed

    Salazar Gómez, J I; Lohmann, H; Krassowski, J

    2016-06-01

    Characterisation of biogases is normally dedicated to the online monitoring of the major components methane and carbon dioxide and, to a lesser extent, to the determination of ammonia and hydrogen sulphide. For the case of Volatile Organic Compounds (VOCs), much less attention is usually paid, since such compounds are normally removed during gas conditioning and with exception of sulphur compounds and siloxanes represent a rather low risk to conventional downstream devices but could be a hindrance for fuel cells. However, there is very little information in the literature about the type of substances found in biogases generated from biowaste or co-fermentation plants and their concentration fluctuations. The main aim of this study was to provide information about the time dependencies of the VOCs in three biogas plants spread out through Germany from autumn until summer, which have different process control, in order to assess their potential as biofuels. Additionally, this study was an attempt to establish a correlation between the nature of the substrates used in the biogas plants and the composition of the VOCs present in the gas phase. Significant time-dependent variations in concentration were observed for most VOCs but only small changes in composition were observed. In general, terpenes and ketones appeared as the predominant VOCs in biogas. Although for substances such as esters, sulphur-organic compounds and siloxanes the average concentrations observed were rather low, they exhibited significant concentration peaks. The second biogas plant which operates with dry fermentation was found to contain the highest levels of VOCs. The amount of total volatile organic compounds (TVOCs) for the first, second and third biogas plants ranged from 35 to 259 mg Nm(-3), 291-1731 mg Nm(-3) and 84-528 mg Nm(-3), respectively. PMID:27010166

  8. Agricultural Plant Pest Control. Manual 93.

    ERIC Educational Resources Information Center

    Missouri Univ., Columbia. Agricultural Experiment Station.

    This training manual provides information needed to meet the minimum EPA standards for certification as a commercial applicator of pesticides for the agricultural plant pest control category. The text discusses the insect pests including caterpillars, beetles, and soil inhabiting insects; diseases and nematodes; and weeds. Consideration is given…

  9. Estimation of potential biomass resource and biogas production from aquatic plants in Argentina

    SciTech Connect

    Fitzsimons, R.E.; Laurino, C.N.; Vallejos, R.H.

    1982-01-01

    The Argentine government's Agua y Energia Electrica is planning to construct a hydroelectric power-generation facility on the middle Parana River, which is already heavily infested with aquatic weeds such as water hyacinth. These species will probably proliferate in the lakes that will be formed by the power project and perhaps seriously interfere with the facility. As a solution to this problem, Argentine biochemists propose mechanical harvesting and anaerobic fermentation of the aquatic plants to produce biogas and fertilizer. According to an evaluation of this potential resource, gross methane production could reach 37-153 billion CF (1.0-4.1 billion m/sup 3/)/yr, and the digested residue could provide 60,500-244,000 tons (54,900-221,400 metric tons)/yr of nitrogen, which represents 2-8 times Argentina's current nitrogen fertilizer demand.

  10. Biogas Potential on Long Island, New York: A Quantification Study

    SciTech Connect

    Mahajan, D.; Patel, S.; Tonjes, D.

    2011-08-25

    Biogas is the product of anaerobic digestion of waste, whether occurring spontaneously in landfills or under controlled conditions in digesters. Biogas is viewed as an important energy source in current efforts to reduce the use of fossil fuels and dependency on imported resources. Several studies on the assessment of biogas potential have been made at regional, national, and global scales. However, because it is not economically feasible to transport biogas feedstock over long distances, it is more appropriate to consider local waste sources for their potential to produce biogas. An assessment of the biogas potential on Long Island, based on the review of local landfills, wastewater treatment plants, solid waste generation and management, and agricultural waste, found that 234 x 10{sup 6} m{sup 3} of methane (CH{sub 4}) from biogas might be harvestable, although substantial barriers for complete exploitation exist. This number is equivalent to 2.52 TW-h of electricity, approximately 12% of fossil fuel power generation on Long Island. This work can serve as a template for other areas to rapidly create or approximate biogas potentials, especially for suburban U.S. locations that are not usually thought of as sources of renewable energy.

  11. Potential assessment of establishing a renewable energy plant in a rural agricultural area.

    PubMed

    Su, Ming-Chien; Kao, Nien-Hsin; Huang, Wen-Jar

    2012-06-01

    An evaluation of the green energy potential generated from biogas and solar power, using agricultural manure waste and a photovoltaic (PV) system, was conducted in a large geographical area of a rural county with low population density and low pollution. The studied area, Shoufeng Township in Hualien County, is located in eastern Taiwan, where a large amount of manure waste is generated from pig farms that are scattered throughout the county. The objective of the study is to assess the possibility of establishing an integrated manure waste treatment plant by using the generated biogas incorporated with the PV system to produce renewable energy and then feed it back to the incorporated farms. A filed investigation, geographic information system (GIS) application, empirical equations development, and RETScreen modeling were conducted in the study. The results indicate that Shoufeng Township has the highest priority in setting up an integrated treatment and renewable energy plant by using GIS mapping within a 10-km radius of the transportation range. Two scenarios were plotted in assessing the renewable energy plant and the estimated electricity generation, plus the greenhouse gas (GHG) reduction was evaluated. Under the current governmental green energy scheme and from a long-term perspective, the assessment shows great potential in establishing the plant, especially in reducing environmental pollution problems, waste treatment, and developing suitable renewable energy. PMID:22788104

  12. Energy potential and alternative usages of biogas and sludge from UASB reactors: case study of the Laboreaux wastewater treatment plant.

    PubMed

    Rosa, A P; Conesa, J A; Fullana, A; Melo, G C B; Borges, J M; Chernicharo, C A L

    2016-01-01

    This work assessed the energy potential and alternative usages of biogas and sludge generated in upflow anaerobic sludge blanket reactors at the Laboreaux sewage treatment plant (STP), Brazil. Two scenarios were considered: (i) priority use of biogas for the thermal drying of dehydrated sludge and the use of the excess biogas for electricity generation in an ICE (internal combustion engine); and (ii) priority use of biogas for electricity generation and the use of the heat of the engine exhaust gases for the thermal drying of the sludge. Scenario 1 showed that the electricity generated is able to supply 22.2% of the STP power demand, but the thermal drying process enables a greater reduction or even elimination of the final volume of sludge to be disposed. In Scenario 2, the electricity generated is able to supply 57.6% of the STP power demand; however, the heat in the exhaust gases is not enough to dry the total amount of dehydrated sludge. PMID:27054741

  13. Villacidro solar demo plant: Integration of small-scale CSP and biogas power plants in an industrial microgrid

    NASA Astrophysics Data System (ADS)

    Camerada, M.; Cau, G.; Cocco, D.; Damiano, A.; Demontis, V.; Melis, T.; Musio, M.

    2016-05-01

    The integration of small scale concentrating solar power (CSP) in an industrial district, in order to develop a microgrid fully supplied by renewable energy sources, is presented in this paper. The plant aims to assess in real operating conditions, the performance, the effectiveness and the reliability of small-scale concentrating solar power technologies in the field of distributed generation. In particular, the potentiality of small scale CSP with thermal storage to supply dispatchable electricity to an industrial microgrid will be investigated. The microgrid will be realized in the municipal waste treatment plant of the Industrial Consortium of Villacidro, in southern Sardinia (Italy), which already includes a biogas power plant. In order to achieve the microgrid instantaneous energy balance, the analysis of the time evolution of the waste treatment plant demand and of the generation in the existing power systems has been carried out. This has allowed the design of a suitable CSP plant with thermal storage and an electrochemical storage system for supporting the proposed microgrid. At the aim of obtaining the expected energy autonomy, a specific Energy Management Strategy, which takes into account the different dynamic performances and characteristics of the demand and the generation, has been designed. In this paper, the configuration of the proposed small scale concentrating solar power (CSP) and of its thermal energy storage, based on thermocline principle, is initially described. Finally, a simulation study of the entire power system, imposing scheduled profiles based on weather forecasts, is presented.

  14. Anaerobic digestion of different feedstocks: impact on energetic and environmental balances of biogas process.

    PubMed

    Bacenetti, Jacopo; Negri, Marco; Fiala, Marco; González-García, Sara

    2013-10-01

    The possibility of limiting the global warming is strictly linked to the reduction of GHG emissions. Renewable energy both allows reducing emissions and permits to delay fossil fuel depletion. The anaerobic digestion of animal manure and energy crops is a promising way of reducing GHG emissions. In Italy agricultural biogas production was considerably increased; nowadays there are about 520 agricultural biogas plants. The increasing number of biogas plants, especially of those larger than 500 kW(e) (electrical power), involves a high consumption of energy crops, large transport distances of biomass and digestate and difficulties on thermal energy valorization. In this study the energetic (CED) and environmental (GHG emissions) profiles associated with the production of electricity derived from biogas have been identified. Three biogas plants located in Northern Italy have been analyzed. The study has been carried out considering a cradle-to-grave perspective and thus, special attention has been paid on the feedstock production and biogas production process. The influences on the results taking into account different plant sizes and feeding rate has been assessed in detail. Energy analysis was performed using the Cumulative Energy Demand method (CED). The climate change was calculated for a 100-year time frame based on GHG emissions indicated as CO2 equivalents (eq) and defined by the IPCC (2006). In comparison to the fossil reference system, the electricity production using biogas saves GHG emissions from 0.188 to 1.193 kg CO2eq per kWh(e). Electricity supply from biogas can also contribute to a considerable reduction of the use of fossil energy carriers (from -3.97 to 10.08 MJ(fossil) per kWh(e)). The electricity production from biogas has a big potential for energy savings and reduction of GHG emissions. Efficient utilization of the cogenerated heat can substantially improve the GHG balance of electricity production from biogas. PMID:23831800

  15. Isolation of acetic, propionic and butyric acid-forming bacteria from biogas plants.

    PubMed

    Cibis, Katharina Gabriela; Gneipel, Armin; König, Helmut

    2016-02-20

    In this study, acetic, propionic and butyric acid-forming bacteria were isolated from thermophilic and mesophilic biogas plants (BGP) located in Germany. The fermenters were fed with maize silage and cattle or swine manure. Furthermore, pressurized laboratory fermenters digesting maize silage were sampled. Enrichment cultures for the isolation of acid-forming bacteria were grown in minimal medium supplemented with one of the following carbon sources: Na(+)-dl-lactate, succinate, ethanol, glycerol, glucose or a mixture of amino acids. These substrates could be converted by the isolates to acetic, propionic or butyric acid. In total, 49 isolates were obtained, which belonged to the phyla Firmicutes, Tenericutes or Thermotogae. According to 16S rRNA gene sequences, most isolates were related to Clostridium sporosphaeroides, Defluviitoga tunisiensis and Dendrosporobacter quercicolus. Acetic, propionic or butyric acid were produced in cultures of isolates affiliated to Bacillus thermoamylovorans, Clostridium aminovalericum, Clostridium cochlearium/Clostridium tetani, C. sporosphaeroides, D. quercicolus, Proteiniborus ethanoligenes, Selenomonas bovis and Tepidanaerobacter sp. Isolates related to Thermoanaerobacterium thermosaccharolyticum produced acetic, butyric and lactic acid, and isolates related to D. tunisiensis formed acetic acid. Specific primer sets targeting 16S rRNA gene sequences were designed and used for real-time quantitative PCR (qPCR). The isolates were physiologically characterized and their role in BGP discussed. PMID:26779817

  16. Biogas production from pear residues using sludge from a wastewater treatment plant digester. Influence of the feed delivery procedure.

    PubMed

    Arhoun, B; Bakkali, A; El Mail, R; Rodriguez-Maroto, J M; Garcia-Herruzo, F

    2013-01-01

    Clear economic advantages may be obtained from the management of seasonal fruit wastes by codigestion at existing facilities which are working throughout the year with other residues. We have explored the biomethanization of pear residues in a 5L stirred reactor loaded with sludge from the anaerobic digester of a municipal wastewater treatment plant. Different organic loading rates (OLRs) of fruit waste were tested with two delivery procedures: a discontinuous one (fed once a day) and a pseudocontinuous one. For both procedures, as the OLR increases the pH of the digester drops to acidic values and large OLRs may cause the reactor failure. Nevertheless, the pseudocontinuous delivery allows the treatment of more residue, (10.5 versus 6.0 g of volatile solids per litre of reactor and day), maintaining the specific biogas production (0.44 L of biogas per gram of volatile solids), with some improvement in methane concentration (44% vs 39%). PMID:23131648

  17. Use of antibiotics in plant agriculture.

    PubMed

    Stockwell, V O; Duffy, B

    2012-04-01

    Antibiotics are essential for control of bacterial diseases of plants, especially fire blight of pear and apple and bacterial spot of peach. Streptomycin is used in several countries; the use of oxytetracycline, oxolinic acid and gentamicin is limited to only a few countries. Springtime antibiotic sprays suppress pathogen growth on flowers and leaf surfaces before infection; after infection, antibiotics are ineffective. Antibiotics are applied when disease risk is high, and consequently the majority of orchards are not treated annually. In 2009 in the United States, 16,465 kg (active ingredient) was applied to orchards, which is 0.12% of the total antibiotics used in animal agriculture. Antibiotics are active on plants for less than a week, and significant residues have not been found on harvested fruit. Antibiotics have been indispensable for crop protection in the United States for more than 50 years without reports of adverse effects on human health or persistent impacts on the environment. PMID:22849276

  18. Microbial Consortium with High Cellulolytic Activity (MCHCA) for Enhanced Biogas Production.

    PubMed

    Poszytek, Krzysztof; Ciezkowska, Martyna; Sklodowska, Aleksandra; Drewniak, Lukasz

    2016-01-01

    The use of lignocellulosic biomass as a substrate in agricultural biogas plants is very popular and yields good results. However, the efficiency of anaerobic digestion, and thus biogas production, is not always satisfactory due to the slow or incomplete degradation (hydrolysis) of plant matter. To enhance the solubilization of the lignocellulosic biomass various physical, chemical and biological pretreatment methods are used. The aim of this study was to select and characterize cellulose-degrading bacteria, and to construct a microbial consortium, dedicated for degradation of maize silage and enhancing biogas production from this substrate. Over 100 strains of cellulose-degrading bacteria were isolated from: sewage sludge, hydrolyzer from an agricultural biogas plant, cattle slurry and manure. After physiological characterization of the isolates, 16 strains (representatives of Bacillus, Providencia, and Ochrobactrum genera) were chosen for the construction of a Microbial Consortium with High Cellulolytic Activity, called MCHCA. The selected strains had a high endoglucanase activity (exceeding 0.21 IU/mL CMCase activity) and a wide range of tolerance to various physical and chemical conditions. Lab-scale simulation of biogas production using the selected strains for degradation of maize silage was carried out in a two-bioreactor system, similar to those used in agricultural biogas plants. The obtained results showed that the constructed MCHCA consortium is capable of efficient hydrolysis of maize silage, and increases biogas production by even 38%, depending on the inoculum used for methane fermentation. The results in this work indicate that the mesophilic MCHCA has a great potential for application on industrial scale in agricultural biogas plants. PMID:27014244

  19. Microbial Consortium with High Cellulolytic Activity (MCHCA) for Enhanced Biogas Production

    PubMed Central

    Poszytek, Krzysztof; Ciezkowska, Martyna; Sklodowska, Aleksandra; Drewniak, Lukasz

    2016-01-01

    The use of lignocellulosic biomass as a substrate in agricultural biogas plants is very popular and yields good results. However, the efficiency of anaerobic digestion, and thus biogas production, is not always satisfactory due to the slow or incomplete degradation (hydrolysis) of plant matter. To enhance the solubilization of the lignocellulosic biomass various physical, chemical and biological pretreatment methods are used. The aim of this study was to select and characterize cellulose-degrading bacteria, and to construct a microbial consortium, dedicated for degradation of maize silage and enhancing biogas production from this substrate. Over 100 strains of cellulose-degrading bacteria were isolated from: sewage sludge, hydrolyzer from an agricultural biogas plant, cattle slurry and manure. After physiological characterization of the isolates, 16 strains (representatives of Bacillus, Providencia, and Ochrobactrum genera) were chosen for the construction of a Microbial Consortium with High Cellulolytic Activity, called MCHCA. The selected strains had a high endoglucanase activity (exceeding 0.21 IU/mL CMCase activity) and a wide range of tolerance to various physical and chemical conditions. Lab-scale simulation of biogas production using the selected strains for degradation of maize silage was carried out in a two-bioreactor system, similar to those used in agricultural biogas plants. The obtained results showed that the constructed MCHCA consortium is capable of efficient hydrolysis of maize silage, and increases biogas production by even 38%, depending on the inoculum used for methane fermentation. The results in this work indicate that the mesophilic MCHCA has a great potential for application on industrial scale in agricultural biogas plants. PMID:27014244

  20. Clostridium cluster I and their pathogenic members in a full-scale operating biogas plant.

    PubMed

    Dohrmann, Anja B; Walz, Meike; Löwen, Achim; Tebbe, Christoph C

    2015-04-01

    A biogas production plant operating with main and secondary digesters (MD, SD) was analysed for the diversity of bacteria from Clostridium cluster I and its pathogenic members. The plant was run in two parallel lines, both receiving silages, and one, in addition, cattle manure (CM). Quantitative PCR of 16S rRNA genes from directly extracted DNA indicated that cluster I represented 0.2 to 5.6 % of the total bacterial communities. Its prevalence was particularly low in CM and also in SD compared to MD, indicating its decline during fermentation. In contrast, another highly abundant clostridial group, i.e. the "faecal" cluster XIVa, remained quantitatively unaffected during fermentation. A total of 85.1 % of 581,934 rRNA gene sequences gathered by group-specific PCR from the silages, CM and digesters could be assigned to cluster I. All remaining sequences fell into other clostridial groups. The three most dominant operational taxonomic units (OTUs) introduced with CM were from cluster I, and they declined during fermentation. Fermentation with CM significantly increased OTUs of clostridia outside of cluster I but not within. The only OTUs related to pathogens were detected for Clostridium botulinum with 0.18 % of all cluster I sequences in maize silage and less than 0.01 % in the other substrates and digester materials. These OTUs could be assigned to all four established C. botulinum groups, thus, potentially covering all seven neurotoxins. Mouse lethality tests of samples with suspected presence of C. botulinum, however, indicated no toxigenic potential and, thus, no risk associated with the rare occurrence of these OTUs. PMID:25472441

  1. Life cycle assessment of flexibly fed biogas processes for an improved demand-oriented biogas supply.

    PubMed

    Ertem, Funda Cansu; Martínez-Blanco, Julia; Finkbeiner, Matthias; Neubauer, Peter; Junne, Stefan

    2016-11-01

    This paper analyses concepts to facilitate a demand oriented biogas supply at an agricultural biogas plant of a capacity of 500kWhel, operated with the co-digestion of maize, grass, rye silage and chicken manure. In contrast to previous studies, environmental impacts of flexible and the traditional baseload operation are compared. Life Cycle Assessment (LCA) was performed to detect the environmental impacts of: (i) variety of feedstock co-digestion scenarios by substitution of maize and (ii) loading rate scenarios with a focus on flexible feedstock utilization. Demand-driven biogas production is critical for an overall balanced power supply to the electrical grid. It results in lower amounts of emissions; feedstock loading rate scenarios resulted in 48%, 20%, 11% lower global warming (GWP), acidification (AP) and eutrophication potentials, and a 16% higher cumulative energy demand. Substitution of maize with biogenic-waste regarding to feedstock substitution scenarios could create 10% lower GWP and AP. PMID:27522120

  2. Large size biogas-fed Solid Oxide Fuel Cell power plants with carbon dioxide management: Technical and economic optimization

    NASA Astrophysics Data System (ADS)

    Curletti, F.; Gandiglio, M.; Lanzini, A.; Santarelli, M.; Maréchal, F.

    2015-10-01

    This article investigates the techno-economic performance of large integrated biogas Solid Oxide Fuel Cell (SOFC) power plants. Both atmospheric and pressurized operation is analysed with CO2 vented or captured. The SOFC module produces a constant electrical power of 1 MWe. Sensitivity analysis and multi-objective optimization are the mathematical tools used to investigate the effects of Fuel Utilization (FU), SOFC operating temperature and pressure on the plant energy and economic performances. FU is the design variable that most affects the plant performance. Pressurized SOFC with hybridization with a gas turbine provides a notable boost in electrical efficiency. For most of the proposed plant configurations, the electrical efficiency ranges in the interval 50-62% (LHV biogas) when a trade-off of between energy and economic performances is applied based on Pareto charts obtained from multi-objective plant optimization. The hybrid SOFC is potentially able to reach an efficiency above 70% when FU is 90%. Carbon capture entails a penalty of more 10 percentage points in pressurized configurations mainly due to the extra energy burdens of captured CO2 pressurization and oxygen production and for the separate and different handling of the anode and cathode exhausts and power recovery from them.

  3. Biogas Opportunities Roadmap Progress Report

    SciTech Connect

    None, None

    2015-12-01

    In support of the Obama Administration's Climate Action Plan, the U.S. Department of Energy, the U.S. Environmental Protection Agency, and U.S. Department of Agriculture jointly released the Biogas Opportunities Roadmap Progress Report, updating the federal government's progress to reduce methane emissions through biogas systems since the Biogas Opportunities Roadmap was completed by the three agencies in July 2014. The report highlights actions taken, outlines challenges and opportunities, and identifies next steps to the growth of a robust biogas industry.

  4. Empedobacter stercoris sp. nov., isolated from an input sample of a biogas plant.

    PubMed

    Schauss, Thorsten; Busse, Hans-Jürgen; Golke, Jan; Kämpfer, Peter; Glaeser, Stefanie P

    2015-10-01

    Two Gram-stain-negative, rod-shaped bacteria, strains 990B6_12ER2AT and 994B6_12ER2A, were isolated during microbiological analysis of a mixed manure sample which was used as input material for a German biogas plant. Phylogenetic identification based on nearly full-length 16S rRNA gene sequences placed the isolates into the family Flavobacteriaceae within the phylum Bacteroidetes. Strains 990B6_12ER2AT and 994B6_12ER2A shared identical 16S rRNA gene sequences and showed highest 16S rRNA gene sequence similarity to the type strains of Empedobacter falsenii (97.3 %) and Empedobacter brevis (96.8 %).The major cellular fatty acids of strains 990B6_12ER2AT and 994B6_12ER2A were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH.The polyamine pattern contained predominantly sym-homospermidine and the quinone system was menaquinone MK-6. Major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified polar lipid not containing an amino residue, a phosphate residue or a sugar moiety. In addition, moderate to minor amounts of several unidentified lipids were detected. The DNA G+C content was 31.7 and 29.0 mol%, for strains 990B6_12ER2AT and 994B6_12ER2A, respectively. On the basis of phylogenetic, chemotaxonomic and physiological analysis we propose a novel species of the genus Empedobacter, Empedobacter stercoris sp. nov. (type strain 990B6_12ER2AT = CIP 110833T = LMG 28501T). PMID:26228269

  5. Refeeding biogas digester solids

    SciTech Connect

    Licht, L.A.

    1981-01-01

    Biosolid, the digester residue from a biogas plant, must be of economical use to ensure the financial feasibility of biogas facilities. This paper sumarizes work performed for a Department of Energy study in the Imperial Valley of California. Feeding trials show that biosolid can only be used as a small proportion of feed rations. Apart from bacterial debris, biosolid is composed larely of non-nutritive residues. 5 refs.

  6. Janata biogas technology and fodder production

    SciTech Connect

    Neelakantan, S.

    1981-01-01

    An effective bio-gas program leads to efficient use of cow dung for gas recovery and partial supplement to plant nutrient requirements. Bio-gas program leads to improvement in rural living including rural sanitation. The Janata biogas plant designed by the State Planning Institute, Lucknow, based on biogas technology, has proved to be efficient and economical. This book contains the various papers presented at the seminar held to review this technology. The various topics covered are: Status of Biogas Program in India; Role of Extension Agencies in Developing Program of Energy Utilization; Introduction to Drumless Biogas Plant; Principles and Application of Anaerobic Fermentation and Biogas Production, Operational System of Gobar Gas in Rural India; Complete Recycling of Cattle Shed Wastes through Biogas Plant; Chemical Composition of Cattle Excreta and Its Manurial Value; Profitability of Biogas Plant; Biogas Production from Various Organic Wastes; Performance of Janata Biogas Plant and Biogas Utilization in Appliances; Utilization of Solar Energy for Domestic Purposes; and Conservation of Forages. Plant requirements and cost estimates have been given for several units.

  7. Biogas Production from Vietnamese Animal Manure, Plant Residues and Organic Waste: Influence of Biomass Composition on Methane Yield

    PubMed Central

    Cu, T. T. T.; Nguyen, T. X.; Triolo, J. M.; Pedersen, L.; Le, V. D.; Le, P. D.; Sommer, S. G.

    2015-01-01

    Anaerobic digestion is an efficient and renewable energy technology that can produce biogas from a variety of biomasses such as animal manure, food waste and plant residues. In developing countries this technology is widely used for the production of biogas using local biomasses, but there is little information about the value of these biomasses for energy production. This study was therefore carried out with the objective of estimating the biogas production potential of typical Vietnamese biomasses such as animal manure, slaughterhouse waste and plant residues, and developing a model that relates methane (CH4) production to the chemical characteristics of the biomass. The biochemical methane potential (BMP) and biomass characteristics were measured. Results showed that piglet manure produced the highest CH4 yield of 443 normal litter (NL) CH4 kg−1 volatile solids (VS) compared to 222 from cows, 177 from sows, 172 from rabbits, 169 from goats and 153 from buffaloes. Methane production from duckweed (Spirodela polyrrhiza) was higher than from lawn grass and water spinach at 340, 220, and 110.6 NL CH4 kg−1 VS, respectively. The BMP experiment also demonstrated that the CH4 production was inhibited with chicken manure, slaughterhouse waste, cassava residue and shoe-making waste. Statistical analysis showed that lipid and lignin are the most significant predictors of BMP. The model was developed from knowledge that the BMP was related to biomass content of lipid, lignin and protein from manure and plant residues as a percentage of VS with coefficient of determination (R-square) at 0.95. This model was applied to calculate the CH4 yield for a household with 17 fattening pigs in the highlands and lowlands of northern Vietnam. PMID:25557826

  8. Biogas production from vietnamese animal manure, plant residues and organic waste: influence of biomass composition on methane yield.

    PubMed

    Cu, T T T; Nguyen, T X; Triolo, J M; Pedersen, L; Le, V D; Le, P D; Sommer, S G

    2015-02-01

    Anaerobic digestion is an efficient and renewable energy technology that can produce biogas from a variety of biomasses such as animal manure, food waste and plant residues. In developing countries this technology is widely used for the production of biogas using local biomasses, but there is little information about the value of these biomasses for energy production. This study was therefore carried out with the objective of estimating the biogas production potential of typical Vietnamese biomasses such as animal manure, slaughterhouse waste and plant residues, and developing a model that relates methane (CH4) production to the chemical characteristics of the biomass. The biochemical methane potential (BMP) and biomass characteristics were measured. Results showed that piglet manure produced the highest CH4 yield of 443 normal litter (NL) CH4 kg(-1) volatile solids (VS) compared to 222 from cows, 177 from sows, 172 from rabbits, 169 from goats and 153 from buffaloes. Methane production from duckweed (Spirodela polyrrhiza) was higher than from lawn grass and water spinach at 340, 220, and 110.6 NL CH4 kg(-1) VS, respectively. The BMP experiment also demonstrated that the CH4 production was inhibited with chicken manure, slaughterhouse waste, cassava residue and shoe-making waste. Statistical analysis showed that lipid and lignin are the most significant predictors of BMP. The model was developed from knowledge that the BMP was related to biomass content of lipid, lignin and protein from manure and plant residues as a percentage of VS with coefficient of determination (R-square) at 0.95. This model was applied to calculate the CH4 yield for a household with 17 fattening pigs in the highlands and lowlands of northern Vietnam. PMID:25557826

  9. Presence of Siloxanes in the Biogas of a Wastewater Treatment Plant Separation in Condensates and Influence of the Dose of Iron Chloride on its Elimination

    SciTech Connect

    Mariano, García; Daniel, Prats; Arturo, Trapote

    2015-12-21

    The siloxanes present in the biogas produced during anaerobic digestion damage the mechanism of cogeneration equipment and, consequently, negatively affect the energy valorization process. For this reason, the detection and elimination of these silicon-derived chemical compounds are a priority in the management of cogeneration facilities. In this regard, the objectives of this paper are, firstly, to characterize the siloxanes in the biogas and, secondly, to qualitatively evaluate the influence of the dose of iron chloride on its elimination. The research was performed at the Rincón de León Wastewater Treatment Plant (Alicante, Spain). The outflow biogas of the digesters and of the pressurized gasometers was sampled and analyzed. The results obtained made it possible to demonstrate, firstly, the absence of linear siloxanes and that, of the cyclic siloxanes, the predominant type was decamethylcyclopentasiloxane, and, secondly, that the addition of iron chloride in the digesters significantly reduces the siloxane content in the biogas. Additionally, it was demonstrated that the process of compression of the biogas, with the elimination of condensates, also produces significant reductions in the concentration of siloxanes in the biogas.

  10. Co-digestion of municipal sludge and external organic wastes for enhanced biogas production under realistic plant constraints.

    PubMed

    Tandukar, Madan; Pavlostathis, Spyros G

    2015-12-15

    A bench-scale investigation was conducted to select external organic wastes and mixing ratios for co-digestion with municipal sludge at the F. Wayne Hill Water Resources Center (FWHWRC), Gwinnett County, GA, USA to support a combined heat and power (CHP) project. External wastes were chosen and used subject to two constraints: a) digester retention time no lower than 15 d; and b) total biogas (methane) production not to exceed a specific target level based on air permit constraints on CO2 emissions. Primary sludge (PS), thickened waste activated sludge (TWAS) and digested sludge collected at the FWHWRC, industrial liquid waste obtained from a chewing gum manufacturing plant (GW) and dewatered fat-oil-grease (FOG) were used. All sludge and waste samples were characterized and their ultimate digestibility was assessed at 35 °C. The ultimate COD to methane conversion of PS, TWAS, municipal sludge (PS + TWAS; 40:60 w/w TS basis), GW and FOG was 49.2, 35.2, 40.3, 72.7, and 81.1%, respectively. Co-digestion of municipal sludge with GW, FOG or both, was evaluated using four bench-scale, mesophilic (35 °C) digesters. Biogas production increased significantly and additional degradation of the municipal sludge between 1.1 and 30.7% was observed. Biogas and methane production was very close to the target levels necessary to close the energy deficit at the FWHWRC. Co-digestion resulted in an effluent quality similar to that of the control digester fed only with the municipal sludge, indicating that co-digestion had no adverse effects. Study results prove that high methane production is achievable with the addition of concentrated external organic wastes to municipal digesters, at acceptable higher digester organic loadings and lower retention times, allowing the effective implementation of CHP programs at municipal wastewater treatment plants, with significant cost savings. PMID:25979784

  11. Effects of geographic area, feedstock, temperature, and operating time on microbial communities of six full-scale biogas plants.

    PubMed

    Fontana, Alessandra; Patrone, Vania; Puglisi, Edoardo; Morelli, Lorenzo; Bassi, Daniela; Garuti, Mirco; Rossi, Lorella; Cappa, Fabrizio

    2016-10-01

    The objective of this study was to investigate the effect of different animal feedings operated in two distinct PDO (protected designation of origin) cheese production areas (Parmigiano Reggiano and Grana Padano) on the microbiome of six full-scale biogas plants, by means of Illumina sequencing and qPCR techniques. The effects of feedstock (cattle slurry manure, energy crops, agro-industrial by-products), temperature (mesophilic/thermophilic), and operating time were also examined, as were the relationships between the predominant bacterial and archaeal taxa and process parameters. The different feedstocks and temperatures strongly affected the microbiomes. A more biodiverse archaeal population was highlighted in Parmigiano Reggiano area plants, suggesting an influence of the different animal feedings. Methanosarcina and Methanosaeta showed an opposite distribution among anaerobic plants, with the former found to be related to ammonium concentration. The Methanoculleus genus was more abundant in the thermophilic digester whereas representation of the Thermotogales order correlated with hydraulic retention time. PMID:27450128

  12. Understanding biogas generation. Technical paper

    SciTech Connect

    Mattocks, R.

    1984-01-01

    Biogas is a by-product of the biological breakdown - under oxygen-free conditions - of organic wastes such as plants, crop residues, wood and bark residues, and human and animal manure. Biogas generators or digesters yield two products: the biogas itself, and a semi-solid by-product called effluent or sludge. Biogas systems are most popular for their ability to produce fuel from products that might otherwise be wasted-crop residues, manures, etc. The fuel is a flammable gas suitable for cooking, lighting, and fueling combustion engines. The digested waste--sludge--is a high quality ferterlizer.

  13. Comparison of strategies for the isolation of PCR-compatible, genomic DNA from a municipal biogas plants.

    PubMed

    Weiss, Agnes; Jérôme, Valérie; Freitag, Ruth

    2007-06-15

    The goal of the project was the extraction of PCR-compatible genomic DNA representative of the entire microbial community from municipal biogas plant samples (mash, bioreactor content, process water, liquid fertilizer). For the initial isolation of representative DNA from the respective lysates, methods were used that employed adsorption, extraction, or precipitation to specifically enrich the DNA. Since no dedicated method for biogas plant samples was available, preference was given to kits/methods suited to samples that resembled either the bioreactor feed, e.g. foodstuffs, or those intended for environmental samples including wastewater. None of the methods succeeded in preparing DNA that was directly PCR-compatible. Instead the DNA was found to still contain considerable amounts of difficult-to-remove enzyme inhibitors (presumably humic acids) that hindered the PCR reaction. Based on the isolation method that gave the highest yield/purity for all sample types, subsequent purification was attempted by agarose gel electrophoresis followed by electroelution, spermine precipitation, or dialysis through nitrocellulose membrane. A combination of phenol/chloroform extraction followed by purification via dialysis constituted the most efficient sample treatment. When such DNA preparations were diluted 1:100 they did no longer inhibit PCR reactions, while they still contained sufficient genomic DNA to allow specific amplification of specific target sequences. PMID:17442638

  14. Biogas utilization

    SciTech Connect

    Moser, M.A.

    1996-01-01

    Options for successfully using biogas depend on project scale. Almost all biogas from anaerobic digesters must first go through a gas handling system that pressurizes, meters, and filters the biogas. Additional treatment, including hydrogen sulfide-mercaptan scrubbing, gas drying, and carbon dioxide removal may be necessary for specialized uses, but these are complex and expensive processes. Thus, they can be justified only for large-scale projects that require high-quality biogas. Small-scale projects (less than 65 cfm) generally use biogas (as produced) as a boiler fuel or for fueling internal combustion engine-generators to produce electricity. If engines or boilers are selected properly, there should be no need to remove hydrogen sulfide. Small-scale combustion turbines, steam turbines, and fuel cells are not used because of their technical complexity and high capital cost. Biogas cleanup to pipeline or transportation fuel specifications is very costly, and energy economics preclude this level of treatment.

  15. Climate balance of biogas upgrading systems

    SciTech Connect

    Pertl, A.; Mostbauer, P.; Obersteiner, G.

    2010-01-15

    One of the numerous applications of renewable energy is represented by the use of upgraded biogas where needed by feeding into the gas grid. The aim of the present study was to identify an upgrading scenario featuring minimum overall GHG emissions. The study was based on a life-cycle approach taking into account also GHG emissions resulting from plant cultivation to the process of energy conversion. For anaerobic digestion two substrates have been taken into account: (1) agricultural resources and (2) municipal organic waste. The study provides results for four different upgrading technologies including the BABIU (Bottom Ash for Biogas Upgrading) method. As the transport of bottom ash is a critical factor implicated in the BABIU-method, different transport distances and means of conveyance (lorry, train) have been considered. Furthermore, aspects including biogas compression and energy conversion in a combined heat and power plant were assessed. GHG emissions from a conventional energy supply system (natural gas) have been estimated as reference scenario. The main findings obtained underlined how the overall reduction of GHG emissions may be rather limited, for example for an agricultural context in which PSA-scenarios emit only 10% less greenhouse gases than the reference scenario. The BABIU-method constitutes an efficient upgrading method capable of attaining a high reduction of GHG emission by sequestration of CO{sub 2}.

  16. Foam formation in biogas plants caused by anaerobic digestion of sugar beet.

    PubMed

    Moeller, Lucie; Lehnig, Marcus; Schenk, Joachim; Zehnsdorf, Andreas

    2015-02-01

    The use of sugar beet in anaerobic digestion (AD) during biogas production can lead to process upsets such as excessive foaming in fermenters. In the present study, foam formation in sugar beet-fed digestates was studied in foaming tests. The increasing disintegration grade of sugar beet was observed to have a promoting effect on foaming in the digestate but did not affect the biogas yield. Chemical analysis of foam and digestate from sugar beet silage AD showed high concentrations of pectin, other carbohydrates and N-containing substances in the foam. Both pectin and sucrose showed little foaming in AD. Nevertheless, sucrose and calcium chloride had a promoting effect on foaming for pectin AD. Salts of divalent ions also enhanced the foam intensity in the case of sugar beet silage AD, whereas ammonium chloride and urea had a lessening effect on sugar beet-based foaming. PMID:25446785

  17. Sample prefractionation with liquid isoelectric focusing enables in depth microbial metaproteome analysis of mesophilic and thermophilic biogas plants.

    PubMed

    Kohrs, F; Heyer, R; Magnussen, A; Benndorf, D; Muth, T; Behne, A; Rapp, E; Kausmann, R; Heiermann, M; Klocke, M; Reichl, U

    2014-10-01

    Biogas production from energy crops and biodegradable waste is one of the major sources for renewable energies in Germany. Within a biogas plant (BGP) a complex microbial community converts biomass to biogas. Unfortunately, disturbances of the biogas process occur occasionally and cause economic losses of varying extent. Besides technical failures the microbial community itself is commonly assumed as a reason for process instability. To improve the performance and efficiency of BGP, a deeper knowledge of the composition and the metabolic state of the microbial community is required and biomarkers for monitoring of process deviations or even the prediction of process failures have to be identified. Previous work based on 2D-electrophoresis demonstrated that the analysis of the metaproteome is well suited to provide insights into the apparent metabolism of the microbial communities. Using SDS-PAGE with subsequent mass spectrometry, stable protein patterns were evaluated for a number of anaerobic digesters. Furthermore, it was shown that severe changes in process parameters such as acidification resulted in significant modifications of the metaproteome. Monitoring of changing protein patterns derived from anaerobic digesters, however, is still a challenge due to the high complexity of the metaproteome. In this study, different combinations of separation techniques to reduce the complexity of proteomic BGP samples were compared with respect to the subsequent identification of proteins by tandem mass spectrometry (MS/MS): (i) 1D: proteins were tryptically digested and the resulting peptides were separated by reversed phase chromatography prior to MS/MS. (ii) 2D: proteins were separated by GeLC-MS/MS according to proteins molecular weights before tryptic digestion, (iii) 3D: proteins were separated by gel-free fractionation using isoelectric focusing (IEF) conducted before GeLC-MS/MS. For this study, a comparison of two anaerobic digesters operated at mesophilic and at

  18. The feasibility of a centralized biogas plant treating the manure produced by an organized animal farmers union in Turkey.

    PubMed

    Dereli, R K; Yangin-Gomec, C; Ozabali, A; Ozturk, I

    2012-01-01

    The aim of this study is to evaluate the feasibility and the energy recovery potential of mesophilic (30-35 °C) anaerobic digestion of animal wastes (manure) at a centralized biogas plant (CBP) for 35,000 cattle. The proposed CBP is composed of an equalization tank followed by pasteurization and 3+[1/2] modules; i.e. each module consists of four completely mixed anaerobic reactors with a capacity of treating the manure from 10,000 cattle. The effect of maize silage loading, as the co-substrate, both on biomethane production and feasibility of the system was also evaluated. Besides, the transport fuel substitutes of the produced biomethane with or without co-substrate were also investigated. Results of the proposed CBP indicated that biomethane production increased ca. 1.65 fold with co-substrate addition and pay-back periods for one module treating 10,000 cattle manure are calculated to be ca. 11 and 7.0 yr without and with silage addition, respectively. Besides, considering the potential revenue when replacing transport fuels, about 74 heavy goods vehicles or 1,560 cars may be powered per year by the biogas produced from the proposed CBP where the co-digestion of manure and maize silage is applied. PMID:22744686

  19. Biogas utilization

    SciTech Connect

    Moser, M.A.

    1995-11-01

    Options for successfully using biomass depend on project scale. Almost all biogas from anaerobic digesters must first go through a gas handling system that pressurizes, meters, and filters the biogas. Additional treatment, including hydrogen sulfide-mercaptan scrubbing, gas drying, and carbon dioxide removal may be necessary for specialized uses, but these are complex and expensive processes. Thus, they can be justified only for large-scale projects that require high-quality biogas. Small-scale projects (less than 65 cfm) generally use biogas (as produced) as a boiler fuel or for fueling internal combustion engine generators to produce electricity. If engines or boilers as selected properly, there should be no need to remove hydrogen sulfide. Small-scale combustion turbines, steam turbines, and fuel cells are not used because of their technical complexity and high capital cost. Biogas cleanup to pipeline or transportation fuel specification is very costly, and energy economics preclude this level of treatment.

  20. Impact of CAD-deficiency in flax on biogas production.

    PubMed

    Wróbel-Kwiatkowska, Magdalena; Jabłoński, Sławomir; Szperlik, Jakub; Dymińska, Lucyna; Łukaszewicz, Marcin; Rymowicz, Waldemar; Hanuza, Jerzy; Szopa, Jan

    2015-12-01

    Global warming and the reduction in our fossil fuel reservoir have forced humanity to look for new means of energy production. Agricultural waste remains a large source for biofuel and bioenergy production. Flax shives are a waste product obtained during the processing of flax fibers. We investigated the possibility of using low-lignin flax shives for biogas production, specifically by assessing the impact of CAD deficiency on the biochemical and structural properties of shives. The study used genetically modified flax plants with a silenced CAD gene, which encodes the key enzyme for lignin synthesis. Reducing the lignin content modified cellulose crystallinity, improved flax shive fermentation and optimized biogas production. Chemical pretreatment of the shive biomass further increased biogas production efficiency. PMID:26178244

  1. Use of digestate from a decentralized on-farm biogas plant as fertilizer in soils: An ecotoxicological study for future indicators in risk and life cycle assessment.

    PubMed

    Pivato, Alberto; Vanin, Stefano; Raga, Roberto; Lavagnolo, Maria Cristina; Barausse, Alberto; Rieple, Antonia; Laurent, Alexis; Cossu, Raffaello

    2016-03-01

    Over the last decade, the number of decentralized farm biogas plants has increased significantly in the EU. This development leads not only to an increasing amount of biogas produced, but also to a higher amount of digestate obtained. One of the most attractive options to manage the digestate is to apply it as biofertiliser to the soil, because this gives the opportunity of recovering the nutrients, primarily nitrogen and phosphorus, and of attenuating the loss of organic matter suffered by soils under agricultural exploitation. Studies have claimed that digestates can present a residual biodegradability, and contain complex organic elements, salts or pathogenic bacteria that can damage terrestrial organisms. However few ecotoxicological studies have been performed to evaluate the ecological impact of digestate application on soil. In this study, the use of digestate as biofertiliser in agriculture was assessed by a battery of ecotoxicological tests considering the potential pollutants present in the digestate as a whole by using the "matrix-based" approach (also known as "whole effluent toxicity" for eluates or wastewater effluents). The direct and indirect tests included plant bioassays with Lepidium sativum, earthworm bioassays with Eisenia fetida, aquatic organisms (Artemia sp. and Daphnia magna) and luminescent bacteria bioassays (Vibrio fischeri). Direct tests occurred to be more sensitive than indirect tests. The earthworm bioassays did not show serious negative effects for concentrations up to 15% (dry weight/dry weight percent, w/w dm) and the plant bioassays showed no negative effect, but rather a positive one for concentrations lower than 20% (w/w dm), which encourages the use of digestate as a biofertiliser in agriculture provided that proper concentrations are used. The indirect tests, on the eluate, with the using aquatic organisms and luminescent bacteria showed an LC50 value of 13.61% volume/volume percent, v/v) for D. magna and no toxicity for

  2. Asexual Plant Reproduction. Agricultural Lesson Plans.

    ERIC Educational Resources Information Center

    Southern Illinois Univ., Carbondale. Dept. of Agricultural Education and Mechanization.

    These lesson plans are intended for use in conducting classes on asexual plant reproduction. Presented first are an attention step/problem statement and a series of questions and answers designed to convey general information about asexual plant reproduction/propagation. The following topics are among those discussed: plant reproduction methods,…

  3. Biogas in Nepal--Retrospects and prospects

    SciTech Connect

    Sood, D.K.

    1983-12-01

    The tremendous need and potential of biogas in Nepal, supported by a very large cattle population, seems to be primarily limited by manpower and budgetary constraints and poor planning and implementation. Besides cooking and lighting, considerable potential, particularly at the rural level exists for biogas powered agro-processing applications such as rice hulling, oil expelling and flour grinding. Experience has shown that such applications, for which equipment is easily available, can be better served by community Biogas plants. The government of Nepal, with assistance from international organizations such as the Asian Development Bank, is strengthening the Biogas Company and taking other appropriate measures to disseminate and establish biogas on a firm footing.

  4. Genomic characterization of Defluviitoga tunisiensis L3, a key hydrolytic bacterium in a thermophilic biogas plant and its abundance as determined by metagenome fragment recruitment.

    PubMed

    Maus, Irena; Cibis, Katharina Gabriela; Bremges, Andreas; Stolze, Yvonne; Wibberg, Daniel; Tomazetto, Geizecler; Blom, Jochen; Sczyrba, Alexander; König, Helmut; Pühler, Alfred; Schlüter, Andreas

    2016-08-20

    The genome sequence of Defluviitoga tunisiensis L3 originating from a thermophilic biogas-production plant was established and recently published as Genome Announcement by our group. The circular chromosome of D. tunisiensis L3 has a size of 2,053,097bp and a mean GC content of 31.38%. To analyze the D. tunisiensis L3 genome sequence in more detail, a phylogenetic analysis of completely sequenced Thermotogae strains based on shared core genes was performed. It appeared that Petrotoga mobilis DSM 10674(T), originally isolated from a North Sea oil-production well, is the closest relative of D. tunisiensis L3. Comparative genome analyses of P. mobilis DSM 10674(T) and D. tunisiensis L3 showed moderate similarities regarding occurrence of orthologous genes. Both genomes share a common set of 1351 core genes. Reconstruction of metabolic pathways important for the biogas production process revealed that the D. tunisiensis L3 genome encodes a large set of genes predicted to facilitate utilization of a variety of complex polysaccharides including cellulose, chitin and xylan. Ethanol, acetate, hydrogen (H2) and carbon dioxide (CO2) were found as possible end-products of the fermentation process. The latter three metabolites are considered to represent substrates for methanogenic Archaea, the key organisms in the final step of the anaerobic digestion process. To determine the degree of relatedness between D. tunisiensis L3 and dominant biogas community members within the thermophilic biogas-production plant, metagenome sequences obtained from the corresponding microbial community were mapped onto the L3 genome sequence. This fragment recruitment revealed that the D. tunisiensis L3 genome is almost completely covered with metagenome sequences featuring high matching accuracy. This result indicates that strains highly related or even identical to the reference strain D. tunisiensis L3 play a dominant role within the community of the thermophilic biogas-production plant. PMID

  5. Agricultural Plant Pest Control. Bulletin 763.

    ERIC Educational Resources Information Center

    French, John C.; And Others

    This manual gives general information on plant pests and pesticides. First, the life-cycle and habits of some common insect pests are given. These include caterpillars, beetles and beetle larvae, and sucking insects. Next, plant diseases such as leaf diseases, wilts, root and crown rots, stem cankers, fruit rots, seed and seedling diseases, and…

  6. Sexual Plant Reproduction. Agricultural Lesson Plans.

    ERIC Educational Resources Information Center

    Southern Illinois Univ., Carbondale. Dept. of Agricultural Education and Mechanization.

    These lesson plans are intended for use in conducting classes on sexual plant reproduction. Presented first are an attention step/problem statement and a series of questions and answers designed to convey general information about sexual plant reproduction/propagation. The following topics are among those discussed: sexual and asexual plant…

  7. Foam formation in a downstream digester of a cascade running full-scale biogas plant: Influence of fat, oil and grease addition and abundance of the filamentous bacterium Microthrix parvicella.

    PubMed

    Lienen, T; Kleyböcker, A; Verstraete, W; Würdemann, H

    2014-02-01

    The microbial community composition in a full-scale biogas plant fed with sewage sludge and fat, oil and grease (FOG) was investigated over a 15-month period, including two foam formation events. Addition of FOG as a substrate in the biogas plant together with high abundances of Microthrix parvicella were found to promote foam formation in the downstream digester of a cascade of two biogas digesters. Genetic fingerprinting and quantitative PCR (qPCR) indicated a higher abundance of M. parvicella in the digester, when the digestion process was accompanied by excessive foaming relative to the reference digesters without disturbance. The creation of foam depended on the introduced proportion of FOG and the abundance of M. parvicella. Furthermore, shifts in the abundance of M. parvicella in the biogas plant were observed within the 15-month monitoring period corresponding to its seasonal abundance in the sludge of the wastewater treatment plant (WWTP). PMID:24321688

  8. Genetic Engineering of Plants. Agricultural Research Opportunities and Policy Concerns.

    ERIC Educational Resources Information Center

    Roberts, Leslie

    Plant scientists and science policymakers from government, private companies, and universities met at a convocation on the genetic engineering of plants. During the convocation, researchers described some of the ways genetic engineering may be used to address agricultural problems. Policymakers delineated and debated changes in research funding…

  9. Lunar base agriculture: Soils for plant growth

    NASA Technical Reports Server (NTRS)

    Ming, Douglas W. (Editor); Henninger, Donald L. (Editor)

    1989-01-01

    This work provides information on research and experimentation concerning various aspects of food production in space and particularly on the moon. Options for human settlement of the moon and Mars and strategies for a lunar base are discussed. The lunar environment, including the mineralogical and chemical properties of lunar regolith are investigated and chemical and physical considerations for a lunar-derived soil are considered. It is noted that biological considerations for such a soil include controlled-environment crop production, both hydroponic and lunar regolith-based; microorganisms and the growth of higher plants in lunar-derived soils; and the role of microbes to condition lunar regolith for plant cultivation. Current research in the controlled ecological life support system (CELSS) project is presented in detail and future research areas, such as the growth of higher research plants in CELSS are considered. Optimum plant and microbiological considerations for lunar derived soils are examined.

  10. [Agriculture microbiology and microbe interaction with plants].

    PubMed

    Caballero-Mellado, Jesús

    2006-01-01

    About the characterization and distribution of novel nitrogen-fixing Burkholderia species associated with maize and other plants and their potential use on the plant growth was presented in this symposium. The symposium included studies directed to the revegetation of eroded areas by using plant growth promoting rhizo-bacteria and mycorrizal fungi associated with desert plants, as well as studies related with the resistance of arbuscular mycorrhizal fungi to heavy metals associated with the environmental pollution. In addition, the identification and characterization of a 31-kb chromosomal fragment from Pseudomonas syringae pv. phaseolicola was presented; such a fragment, involved with the phaseolotoxin synthesis, showed characteristic features of a bacterial pathogenicity island. PMID:17578087

  11. Agriculture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Agriculture within the United States is varied and produces a large value ($200 billion in 2002) of production across a wide range of plant and animal production systems. Because of this diversity, changes in climate will likely impact agriculture throughout the United States. Climate affects crop, ...

  12. Mass flow and energy balance plus economic analysis of a full-scale biogas plant in the rice-wine-pig system.

    PubMed

    Li, Jiang; Kong, Chuixue; Duan, Qiwu; Luo, Tao; Mei, Zili; Lei, Yunhui

    2015-10-01

    This paper presents mass flow and energy balance as well as an economic analysis for a biogas plant in a rice-wine-pig system at a practical rather than laboratory scale. Results showed feeding amount was 65.30 t d(-1) (total solid matter (TSM) 1.3%) for the normal temperature continuous stirred tank reactor (CSTR), and 16.20 t d(-1) (TSM 8.4%) for the mesophilic CSTR. The digestion produced 80.50 t d(-1) of mass, with 76.41 t d(-1) flowing into rice fields and 4.49 t d(-1) into composting. Energy consumption of this plant fluctuated with seasons, and surplus energy was 823, 221 kWh/year. Thus, biogas plant was critical for material recycling and energy transformation of this agro-ecosystem. The economic analysis showed that the payback time of the plant was 10.9 years. It also revealed application of biogas as a conventional energy replacement would be attractive for a crop-wine-livestock ecosystem with anaerobic digestion of manure. PMID:26117236

  13. Anaerobic co-digestion plants for the revaluation of agricultural waste: Sustainable location sites from a GIS analysis.

    PubMed

    Villamar, Cristina Alejandra; Rivera, Diego; Aguayo, Mauricio

    2016-04-01

    The aim of this study was to establish sustainably feasible areas for the implementation of anaerobic co-digestion plants for agricultural wastes (cattle/swine slurries and cereal crop wastes). The methodology was based on the use of geographic information systems (GIS), the analytic hierarchy process (AHP) and map algebra generated from hedges related to environmental, social and economic constraints. The GIS model obtained was applied to a region of Chile (Bío Bío Region) as a case study showing the energy potential (205 MW-h) of agricultural wastes (swine/cattle manures and cereal crop wastes) and thereby assessing its energy contribution (3.5%) at country level (Chile). From this model, it was possible to spatially identify the influence of each factor (environmental, economic and social) when defining suitable areas for the siting of anaerobic co-digestion plants. In conclusion, GIS-based models establish appropriate areas for the location of anaerobic co-digestion plants in the revaluation of agricultural waste from the production of energy through biogas production. PMID:26862147

  14. Enzyme research and applications in biotechnological intensification of biogas production.

    PubMed

    Parawira, Wilson

    2012-06-01

    Biogas technology provides an alternative source of energy to fossil fuels in many parts of the world. Using local resources such as agricultural crop remains, municipal solid wastes, market wastes and animal waste, energy (biogas), and manure are derived by anaerobic digestion. The hydrolysis process, where the complex insoluble organic materials are hydrolysed by extracellular enzymes, is a rate-limiting step for anaerobic digestion of high-solid organic solid wastes. Biomass pretreatment and hydrolysis are areas in need of drastic improvement for economic production of biogas from complex organic matter such as lignocellulosic material and sewage sludge. Despite development of pretreatment techniques, sugar release from complex biomass still remains an expensive and slow step, perhaps the most critical in the overall process. This paper gives an updated review of the biotechnological advances to improve biogas production by microbial enzymatic hydrolysis of different complex organic matter for converting them into fermentable structures. A number of authors have reported significant improvement in biogas production when crude and commercial enzymes are used in the pretreatment of complex organic matter. There have been studies on the improvement of biogas production from lignocellulolytic materials, one of the largest and renewable sources of energy on earth, after pretreatment with cellulases and cellulase-producing microorganisms. Lipids (characterised as oil, grease, fat, and free long chain fatty acids, LCFA) are a major organic compound in wastewater generated from the food processing industries and have been considered very difficult to convert into biogas. Improved methane yield has been reported in the literature when these lipid-rich wastewaters are pretreated with lipases and lipase-producing microorganisms. The enzymatic treatment of mixed sludge by added enzymes prior to anaerobic digestion has been shown to result in improved degradation of the

  15. Identification of different species of Bacillus isolated from Nisargruna Biogas Plant by FTIR, UV-Vis and NIR spectroscopy

    NASA Astrophysics Data System (ADS)

    Ghosh, S. B.; Bhattacharya, K.; Nayak, S.; Mukherjee, P.; Salaskar, D.; Kale, S. P.

    2015-09-01

    Definitive identification of microorganisms, including pathogenic and non-pathogenic bacteria, is extremely important for a wide variety of applications including food safety, environmental studies, bio-terrorism threats, microbial forensics, criminal investigations and above all disease diagnosis. Although extremely powerful techniques such as those based on PCR and microarrays exist, they require sophisticated laboratory facilities along with elaborate sample preparation by trained researchers. Among different spectroscopic techniques, FTIR was used in the 1980s and 90s for bacterial identification. In the present study five species of Bacillus were isolated from the aerobic predigester chamber of Nisargruna Biogas Plant (NBP) and were identified to the species level by biochemical and molecular biological (16S ribosomal DNA sequence) methods. Those organisms were further checked by solid state spectroscopic absorbance measurements using a wide range of electromagnetic radiation (wavelength 200 nm to 25,000 nm) encompassing UV, visible, near Infrared and Infrared regions. UV-Vis and NIR spectroscopy was performed on dried bacterial cell suspension on silicon wafer in specular mode while FTIR was performed on KBr pellets containing the bacterial cells. Consistent and reproducible species specific spectra were obtained and sensitivity up to a level of 1000 cells was observed in FTIR with a DTGS detector. This clearly shows the potential of solid state spectroscopic techniques for simple, easy to implement, reliable and sensitive detection of bacteria from environmental samples.

  16. Cultivation of vancomycin-resistant enterococci and methicillin-resistant staphylococci from input and output samples of German biogas plants.

    PubMed

    Glaeser, Stefanie P; Sowinsky, Olivia; Brunner, Jana S; Dott, Wolfgang; Kämpfer, Peter

    2016-03-01

    Vancomycin-resistant enterococci (VRE) and methicillin-resistant staphylococci (MRS) were detected in two mesophilic German biogas plants (BGPs) using selective pre-enrichment methods combined with cultivation on CHROMagar media and antibiotic resistance gene screening. Genetic fingerprinting and 16S rRNA gene sequencing showed the presence of enterococci isolated by the VRE selective cultivation (67 isolates) in input and output samples of BGPs. In contrast, MRS (44 isolates) were detected in input, but in none of the output samples. Enterococcus isolates showed highest 16S rRNA gene sequence similarity (>99.8%) to E. lemanii, E. casseliflavus/E. gallinarium or E. devriesei/E. pseudoavium/E. viikkiensis and carried vanA, vanB and/or vanC1 genes. Enterococcus faecium and E. faecalis VRE were not detected, but isolates closely related to those species (>99.9% 16S rRNA gene sequence similarity) were detected by the MRS selective cultivation methods. Staphylococcus isolates shared highest 16S rRNA gene sequence similarity (>99.9%) with S. haemolyticus, S. lentus and S. sciuri and carried mecA genes. Methicillin-resistant S. aureus (MRSA) were not detected. In summary, manure from livestock husbandry contained both, VRE and MRS. VRE were also detected in output samples, indicating that enterococci with vancomycin resistance genes could be release into the environment by the application of BGP output material as biofertilizers. PMID:26790463

  17. Fate of the Cry1Ab protein from Bt-maize MON810 silage in biogas production facilities.

    PubMed

    Rauschen, Stefan; Schuphan, Ingolf

    2006-02-01

    Biogas plants fuelled with renewable sources of energy are a sustainable means for power generation. In areas with high infestation levels with the European corn borer, Ostrinia nubilalis (Hbn.), it is likely that transgenic Bt-maize will be fed into agricultural biogas plants. The fate of the entomotoxic protein Cry1Ab from MON810 maize was therefore investigated in silage and biogas production-related materials in the utilization chains of two farm-scale biogas plants. The Cry1Ab content in silage exhibited no clear-cut pattern of decrease over the experimental time of 4 months. Mean content for silage was 1878 +/- 713 ng Cry1Ab g(-1). After fermentation in the biogas plants, the Cry1Ab content declined to trace amounts of around 3.5 ng g(-1) in the effluents. The limit of detection of the employed ELISA test corresponded to 0.75 ng Cry1Ab g(-1) sample material. Assays with larvae of O. nubilalis showed no bioactivity of the reactor effluents. The utilization of this residual material as fertilizer in agriculture is therefore deemed to be ecotoxicologically harmless. PMID:16448198

  18. Entomopathogenic and plant pathogenic nematodes as opposing forces in agriculture.

    PubMed

    Kenney, Eric; Eleftherianos, Ioannis

    2016-01-01

    Plant-parasitic nematodes are responsible for substantial damages within the agriculture industry every year, which is a challenge that has thus far gone largely unimpeded. Chemical nematicides have been employed with varying degrees of success, but their implementation can be cumbersome, and furthermore they could potentially be neutralising an otherwise positive effect from the entomopathogenic nematodes that coexist with plant-parasitic nematodes in soil environments and provide protection for plants against insect pests. Recent research has explored the potential of employing entomopathogenic nematodes to protect plants from plant-parasitic nematodes, while providing their standard degree of protection against insects. The interactions involved are highly complex, due to both the three-organism system and the assortment of variables present in a soil environment, but a strong collection of evidence has accumulated regarding the suppressive capacity of certain entomopathogenic nematodes and their mutualistic bacteria, in the context of limiting the infectivity of plant-parasitic nematodes. Specific factors produced by certain entomopathogenic nematode complexes during the process of insect infection appear to have a selectively nematicidal, or at least repellant, effect on plant-parasitic nematodes. Using this information, an opportunity has formed to adapt this relationship to large-scale, field conditions and potentially relieve the agricultural industry of one of its most substantial burdens. PMID:26527129

  19. Demonstration of anaerobic biogas digesters in developing countries. Part III. The Philippines

    SciTech Connect

    Simpson, M.H.; Morales, E.C.

    1980-03-01

    The main theme of this series of articles is that ours is now a world-wide society, short on meeting needs for energy yet long on waste from our industrial, agricultural and human consumption processes. This is a study report about developments in the Philippines where waste management has been recognized and considered as an important practical source of energy. This is revealed by several reports of the number of biogas plants in operation in this country. According to the July 31, 1977 survey made by the Philippines Bureau of Animal Industries, 200 biogas plants were then installed and in operation of which 46 were government-owned and 154 privately-owned. More have been installed since then. This report presents some of the operating observations and developments from the joint engineering analyses project of the Philippines Bureau of Animal Industry, Man and the Biosphere Inter-Agency Committee on Ecological Studies, Bureau of Fisheries and Aquatic Resources and the National Institute of Science and Technology. The project's main objective was to show that establishing a biogas plant involves not only the production of a methane gas mixture but the integration of its other products as part of a system (i.e., using effluent water from the biogas digester for production of algae chlorell sp. for livestock and poultry feed, production of fish and fertilizing-irrigating of pasture and vegetable plots.). Housing development sewer systems with added biogas generators are also discussed.

  20. Cytogenetic changes induced by aqueous ferrofluids in agricultural plants

    NASA Astrophysics Data System (ADS)

    Răcuciu, Mihaela; Creangă, Dorina

    2007-04-01

    In this paper, the authors present their results regarding the cellular division rate and the percentage of chromosomal aberrations in the root meristematic cells of agricultural plants when cultivated in the presence of different concentrations of aqueous ferrofluid, ranging between 10 and 250 μL/L. The agricultural species ( Zea mays) with a major role in the life of people was chosen for the experimental project. The water-based ferrofluid was prepared following the chemical co-precipitation method, using tetramethylammonium hydroxide as magnetite core stabilizer. Microscopic investigations (cytogenetic tests) resulted in the evaluation of the mitotic and chromosomal aberration index. They appeared to increase following ferrofluid addition.

  1. Plant genetics, sustainable agriculture and global food security.

    PubMed

    Ronald, Pamela

    2011-05-01

    The United States and the world face serious societal challenges in the areas of food, environment, energy, and health. Historically, advances in plant genetics have provided new knowledge and technologies needed to address these challenges. Plant genetics remains a key component of global food security, peace, and prosperity for the foreseeable future. Millions of lives depend upon the extent to which crop genetic improvement can keep pace with the growing global population, changing climate, and shrinking environmental resources. While there is still much to be learned about the biology of plant-environment interactions, the fundamental technologies of plant genetic improvement, including crop genetic engineering, are in place, and are expected to play crucial roles in meeting the chronic demands of global food security. However, genetically improved seed is only part of the solution. Such seed must be integrated into ecologically based farming systems and evaluated in light of their environmental, economic, and social impacts-the three pillars of sustainable agriculture. In this review, I describe some lessons learned, over the last decade, of how genetically engineered crops have been integrated into agricultural practices around the world and discuss their current and future contribution to sustainable agricultural systems. PMID:21546547

  2. Evaluation of Mediterranean Agricultural Residues as a Potential Feedstock for the Production of Biogas via Anaerobic Fermentation.

    PubMed

    Nitsos, Christos; Matsakas, Leonidas; Triantafyllidis, Kostas; Rova, Ulrika; Christakopoulos, Paul

    2015-01-01

    Hydrothermal, dilute acid, and steam explosion pretreatment methods, were evaluated for their efficiency to improve the methane production yield of three Mediterranean agricultural lignocellulosic residues such as olive tree pruning, grapevine pruning, and almond shells. Hydrothermal and dilute acid pretreatments provided low to moderate increase in the digestibility of the biomass samples, whereas steam explosion enabled the highest methane yields to be achieved for almond shells at 232.2 ± 13.0 mL CH4/gVS and olive pruning at 315.4 ± 0.0 mL CH4/gVS. Introduction of an enzymatic prehydrolysis step moderately improved methane yields for hydrothermal and dilute acid pretreated samples but not for the steam exploded ones. PMID:26609521

  3. Evaluation of Mediterranean Agricultural Residues as a Potential Feedstock for the Production of Biogas via Anaerobic Fermentation

    PubMed Central

    Nitsos, Christos; Matsakas, Leonidas; Triantafyllidis, Kostas; Rova, Ulrika; Christakopoulos, Paul

    2015-01-01

    Hydrothermal, dilute acid, and steam explosion pretreatment methods, were evaluated for their efficiency to improve the methane production yield of three Mediterranean agricultural lignocellulosic residues such as olive tree pruning, grapevine pruning, and almond shells. Hydrothermal and dilute acid pretreatments provided low to moderate increase in the digestibility of the biomass samples, whereas steam explosion enabled the highest methane yields to be achieved for almond shells at 232.2 ± 13.0 mL CH4/gVS and olive pruning at 315.4 ± 0.0 mL CH4/gVS. Introduction of an enzymatic prehydrolysis step moderately improved methane yields for hydrothermal and dilute acid pretreated samples but not for the steam exploded ones. PMID:26609521

  4. Toward Martian agriculture: responses of plants to hypobaria

    NASA Technical Reports Server (NTRS)

    Corey, Kenneth A.; Barta, Daniel J.; Wheeler, Raymond M.

    2002-01-01

    The recent surge of interest in human missions to Mars has also generated considerable interest in the responses of plants to hypobaria (reduced atmospheric pressure), particularly among those in the advanced life support community. Potential for in situ resource utilization, challenges in meeting engineering constraints for mass and energy, the prospect of using lightweight plant growth structures on Mars, and the minimal literature on plant responses to low pressure all suggest much needed research in this area. However, the limited literature on hypobaria combined with previous findings on plant responses to atmospheric composition and established principles of mass transfer of gases suggest that some plants will be capable of tolerating and growing at pressures below 20 kPa; and for other species, perhaps as low as 5-10 kPa. In addition, normal and perhaps enhanced growth of many plants will likely occur at reduced partial pressures of oxygen (e.g., 5 kPa). Growth of plants at such low and partial pressures indicates the feasibility of cultivating plants in lightweight, transparent "greenhouses" on the surface of Mars or in other extraterrestrial or extreme environment locations. There are numerous, accessible terrestrial analogs for moderately low pressure ranges, but not for very low and extremely low atmospheric pressures. Research pertaining to very low pressures has been historically restricted to the use of vacuum chambers. Future research prospects, approaches, and priorities for plant growth experiments at low pressure are considered and discussed as they apply to prospects for Martian agriculture.

  5. Modeling Halophytic Plants in APEX for Sustainable Water and Agriculture

    NASA Astrophysics Data System (ADS)

    DeRuyter, T.; Saito, L.; Nowak, B.; Rossi, C.; Toderich, K.

    2013-12-01

    A major problem for irrigated agricultural production is soil salinization, which can occur naturally or can be human-induced. Human-induced, or secondary salinization, is particularly a problem in arid and semi-arid regions, especially in irrigated areas. Irrigated land has more than twice the production of rainfed land, and accounts for about one third of the world's food, but nearly 20% of irrigated lands are salt-affected. Many farmers worldwide currently seasonally leach their land to reduce the soil salt content. These practices, however, create further problems such as a raised groundwater table, and salt, fertilizer, and pesticide pollution of nearby lakes and groundwater. In Uzbekistan, a combination of these management practices and a propensity to cultivate 'thirsty' crops such as cotton has also contributed to the Aral Sea shrinking nearly 90% by volume since the 1950s. Most common agricultural crops are glycophytes that have reduced yields when subjected to salt-stress. Some plants, however, are known as halophytic or 'salt-loving' plants and are capable of completing their life-cycle in higher saline soil or water environments. Halophytes may be useful for human consumption, livestock fodder, or biofuel, and may also be able to reduce or maintain salt levels in soil and water. To assess the potential for these halophytes to assist with salinity management, we are developing a model that is capable of tracking salinity under different management practices in agricultural environments. This model is interdisciplinary as it combines fields such as plant ecology, hydrology, and soil science. The US Department of Agriculture (USDA) model, Agricultural Policy/Environmental Extender (APEX), is being augmented with a salinity module that tracks salinity as separate ions across the soil-plant-water interface. The halophytes Atriplex nitens, Climacoptera lanata, and Salicornia europaea are being parameterized and added into the APEX model database. Field sites

  6. Cost analysis of concepts for a demand oriented biogas supply for flexible power generation.

    PubMed

    Hahn, Henning; Ganagin, Waldemar; Hartmann, Kilian; Wachendorf, Michael

    2014-10-01

    With the share of intermittent renewable energies within the electricity system rising, balancing services from dispatchable power plants are of increasing importance. Highlighting the importance of the need to keeping fuel costs for flexible power generation to a minimum, the study aims to identify favourable biogas plant configurations, supplying biogas on demand. A cost analysis of five configurations based on biogas storing and flexible biogas production concepts has been carried out. Results show that additional flexibility costs for a biogas supply of 8h per day range between 2€ and 11€MWh(-1) and for a 72h period without biogas demand from 9€ to 19€MWh(-1). While biogas storage concepts were identified as favourable short term supply configurations, flexible biogas production concepts profit from reduced storage requirements at plants with large biogas production capacities or for periods of several hours without biogas demand. PMID:25146313

  7. Plant Genetics, Sustainable Agriculture and Global Food Security

    PubMed Central

    Ronald, Pamela

    2011-01-01

    The United States and the world face serious societal challenges in the areas of food, environment, energy, and health. Historically, advances in plant genetics have provided new knowledge and technologies needed to address these challenges. Plant genetics remains a key component of global food security, peace, and prosperity for the foreseeable future. Millions of lives depend upon the extent to which crop genetic improvement can keep pace with the growing global population, changing climate, and shrinking environmental resources. While there is still much to be learned about the biology of plant–environment interactions, the fundamental technologies of plant genetic improvement, including crop genetic engineering, are in place, and are expected to play crucial roles in meeting the chronic demands of global food security. However, genetically improved seed is only part of the solution. Such seed must be integrated into ecologically based farming systems and evaluated in light of their environmental, economic, and social impacts—the three pillars of sustainable agriculture. In this review, I describe some lessons learned, over the last decade, of how genetically engineered crops have been integrated into agricultural practices around the world and discuss their current and future contribution to sustainable agricultural systems. PMID:21546547

  8. Plant growth promotion in cereal and leguminous agricultural important plants: from microorganism capacities to crop production.

    PubMed

    Pérez-Montaño, F; Alías-Villegas, C; Bellogín, R A; del Cerro, P; Espuny, M R; Jiménez-Guerrero, I; López-Baena, F J; Ollero, F J; Cubo, T

    2014-01-01

    Plant growth-promoting rhizobacteria (PGPR) are free-living bacteria which actively colonize plant roots, exerting beneficial effects on plant development. The PGPR may (i) promote the plant growth either by using their own metabolism (solubilizing phosphates, producing hormones or fixing nitrogen) or directly affecting the plant metabolism (increasing the uptake of water and minerals), enhancing root development, increasing the enzymatic activity of the plant or "helping" other beneficial microorganisms to enhance their action on the plants; (ii) or may promote the plant growth by suppressing plant pathogens. These abilities are of great agriculture importance in terms of improving soil fertility and crop yield, thus reducing the negative impact of chemical fertilizers on the environment. The progress in the last decade in using PGPR in a variety of plants (maize, rice, wheat, soybean and bean) along with their mechanism of action are summarized and discussed here. PMID:24144612

  9. Transferable antibiotic resistance plasmids from biogas plant digestates often belong to the IncP-1ε subgroup

    PubMed Central

    Wolters, Birgit; Kyselková, Martina; Krögerrecklenfort, Ellen; Kreuzig, Robert; Smalla, Kornelia

    2015-01-01

    Manure is known to contain residues of antibiotics administered to farm animals as well as bacteria carrying antibiotic resistance genes (ARGs). These genes are often located on mobile genetic elements. In biogas plants (BGPs), organic substrates such as manure and plant material are mixed and fermented in order to provide energy, and resulting digestates are used for soil fertilization. The fate of plasmid carrying bacteria from manure during the fermentation process is unknown. The present study focused on transferable antibiotic resistance plasmids from digestates of seven BGPs, using manure as a co-substrate, and their phenotypic and genotypic characterization. Plasmids conferring resistance to either tetracycline or sulfadiazine were captured by means of exogenous plasmid isolation from digestates into Pseudomonas putida KT2442 and Escherichia coli CV601 recipients, at transfer frequencies ranging from 10-5 to 10-7. Transconjugants (n = 101) were screened by PCR-Southern blot hybridization and real-time PCR for the presence of IncP-1, IncP-1ε, IncW, IncN, IncP-7, IncP-9, LowGC, and IncQ plasmids. While 61 plasmids remained unassigned, 40 plasmids belonged to the IncP-1ε subgroup. All these IncP-1ε plasmids were shown to harbor the genes tet(A), sul1, qacEΔ1, intI1, and integron gene cassette amplicons of different size. Further analysis of 16 representative IncP-1ε plasmids showed that they conferred six different multiple antibiotic resistance patterns and their diversity seemed to be driven by the gene cassette arrays. IncP-1ε plasmids displaying similar restriction and antibiotic resistance patterns were captured from different BGPs, suggesting that they may be typical of this environment. Our study showed that BGP digestates are a potential source of transferable antibiotic resistance plasmids, and in particular the broad host range IncP-1ε plasmids might contribute to the spread of ARGs when digestates are used as fertilizer. PMID:25653641

  10. Current challenges and future perspectives of plant and agricultural biotechnology.

    PubMed

    Moshelion, Menachem; Altman, Arie

    2015-06-01

    Advances in understanding plant biology, novel genetic resources, genome modification, and omics technologies generate new solutions for food security and novel biomaterials production under changing environmental conditions. New gene and germplasm candidates that are anticipated to lead to improved crop yields and other plant traits under stress have to pass long development phases based on trial and error using large-scale field evaluation. Therefore, quantitative, objective, and automated screening methods combined with decision-making algorithms are likely to have many advantages, enabling rapid screening of the most promising crop lines at an early stage followed by final mandatory field experiments. The combination of novel molecular tools, screening technologies, and economic evaluation should become the main goal of the plant biotechnological revolution in agriculture. PMID:25842169

  11. Not all GMOs are crop plants: non-plant GMO applications in agriculture.

    PubMed

    Hokanson, K E; Dawson, W O; Handler, A M; Schetelig, M F; St Leger, R J

    2014-12-01

    Since tools of modern biotechnology have become available, the most commonly applied and often discussed genetically modified organisms are genetically modified crop plants, although genetic engineering is also being used successfully in organisms other than plants, including bacteria, fungi, insects, and viruses. Many of these organisms, as with crop plants, are being engineered for applications in agriculture, to control plant insect pests or diseases. This paper reviews the genetically modified non-plant organisms that have been the subject of permit approvals for environmental release by the United States Department of Agriculture/Animal and Plant Health Inspection Service since the US began regulating genetically modified organisms. This is an indication of the breadth and progress of research in the area of non-plant genetically modified organisms. This review includes three examples of promising research on non-plant genetically modified organisms for application in agriculture: (1) insects for insect pest control using improved vector systems; (2) fungal pathogens of insects to control insect pests; and (3) virus for use as transient-expression vectors for disease control in plants. PMID:24242193

  12. Soil-plant transfer of Cs-137 and Sr-90 in digestate amended agricultural soils- a lysimeter scale experiment

    NASA Astrophysics Data System (ADS)

    Mehmood, Khalid; Berns, Anne E.; Pütz, Thomas; Burauel, Peter; Vereecken, Harry; Zoriy, Myroslav; Flucht, Reinhold; Opitz, Thorsten; Hofmann, Diana

    2014-05-01

    Radiocesium and radiostrontium are among the most problematic soil contaminants following nuclear fallout due to their long half-lives and high fission yields. Their chemical resemblance to potassium, ammonium and calcium facilitates their plant uptake and thus enhances their chance to reach humans through the food-chain dramatically. The plant uptake of both radionuclides is affected by the type of soil, the amount of organic matter and the concentration of competitive ions. In the present lysimeter scale experiment, soil-plant transfer of Cs-137 and Sr-90 was investigated in an agricultural silty soil amended with digestate, a residue from a biogas plant. The liquid fraction of the digestate, liquor, was used to have higher nutrient competition. Digestate application was done in accordance with the field practice with an application rate of 34 Mg/ha and mixing it in top 5 cm soil, yielding a final concentration of 38 g digestate/Kg soil. The top 5 cm soil of the non-amended reference soil was also submitted to the same mixing procedure to account for the physical disturbance of the top soil layer. Six months after the amendment of the soil, the soil contamination was done with water-soluble chloride salts of both radionuclides, resulting in a contamination density of 66 MBq/m2 for Cs-137 and 18 MBq/m2 for Sr-90 in separate experiments. Our results show that digestate application led to a detectable difference in soil-plant transfer of the investigated radionuclides, effect was more pronounced for Cs-137. A clear difference was observed in plant uptake of different plants. Pest plants displayed higher uptake of both radionuclides compared to wheat. Furthermore, lower activity values were recorded in ears compared to stems for both radionuclides.

  13. Ultrasonic Sensing of Plant Water Needs for Agriculture

    PubMed Central

    Gómez Álvarez-Arenas, Tomas; Gil-Pelegrin, Eustaquio; Ealo Cuello, Joao; Fariñas, Maria Dolores; Sancho-Knapik, Domingo; Collazos Burbano, David Alejandro; Peguero-Pina, Jose Javier

    2016-01-01

    Fresh water is a key natural resource for food production, sanitation and industrial uses and has a high environmental value. The largest water use worldwide (~70%) corresponds to irrigation in agriculture, where use of water is becoming essential to maintain productivity. Efficient irrigation control largely depends on having access to reliable information about the actual plant water needs. Therefore, fast, portable and non-invasive sensing techniques able to measure water requirements directly on the plant are essential to face the huge challenge posed by the extensive water use in agriculture, the increasing water shortage and the impact of climate change. Non-contact resonant ultrasonic spectroscopy (NC-RUS) in the frequency range 0.1–1.2 MHz has revealed as an efficient and powerful non-destructive, non-invasive and in vivo sensing technique for leaves of different plant species. In particular, NC-RUS allows determining surface mass, thickness and elastic modulus of the leaves. Hence, valuable information can be obtained about water content and turgor pressure. This work analyzes and reviews the main requirements for sensors, electronics, signal processing and data analysis in order to develop a fast, portable, robust and non-invasive NC-RUS system to monitor variations in leaves water content or turgor pressure. A sensing prototype is proposed, described and, as application example, used to study two different species: Vitis vinifera and Coffea arabica, whose leaves present thickness resonances in two different frequency bands (400–900 kHz and 200–400 kHz, respectively), These species are representative of two different climates and are related to two high-added value agricultural products where efficient irrigation management can be critical. Moreover, the technique can also be applied to other species and similar results can be obtained. PMID:27428968

  14. Ultrasonic Sensing of Plant Water Needs for Agriculture.

    PubMed

    Gómez Álvarez-Arenas, Tomas; Gil-Pelegrin, Eustaquio; Ealo Cuello, Joao; Fariñas, Maria Dolores; Sancho-Knapik, Domingo; Collazos Burbano, David Alejandro; Peguero-Pina, Jose Javier

    2016-01-01

    Fresh water is a key natural resource for food production, sanitation and industrial uses and has a high environmental value. The largest water use worldwide (~70%) corresponds to irrigation in agriculture, where use of water is becoming essential to maintain productivity. Efficient irrigation control largely depends on having access to reliable information about the actual plant water needs. Therefore, fast, portable and non-invasive sensing techniques able to measure water requirements directly on the plant are essential to face the huge challenge posed by the extensive water use in agriculture, the increasing water shortage and the impact of climate change. Non-contact resonant ultrasonic spectroscopy (NC-RUS) in the frequency range 0.1-1.2 MHz has revealed as an efficient and powerful non-destructive, non-invasive and in vivo sensing technique for leaves of different plant species. In particular, NC-RUS allows determining surface mass, thickness and elastic modulus of the leaves. Hence, valuable information can be obtained about water content and turgor pressure. This work analyzes and reviews the main requirements for sensors, electronics, signal processing and data analysis in order to develop a fast, portable, robust and non-invasive NC-RUS system to monitor variations in leaves water content or turgor pressure. A sensing prototype is proposed, described and, as application example, used to study two different species: Vitis vinifera and Coffea arabica, whose leaves present thickness resonances in two different frequency bands (400-900 kHz and 200-400 kHz, respectively), These species are representative of two different climates and are related to two high-added value agricultural products where efficient irrigation management can be critical. Moreover, the technique can also be applied to other species and similar results can be obtained. PMID:27428968

  15. Renewable Hydrogen Potential from Biogas in the United States

    SciTech Connect

    Saur, G.; Milbrandt, A.

    2014-07-01

    This analysis updates and expands upon previous biogas studies to include total potential and net availability of methane in raw biogas with respect to competing demands and includes a resource assessment of four sources of biogas: (1) wastewater treatment plants, including domestic and a new assessment of industrial sources; (2) landfills; (3) animal manure; and (4) a new assessment of industrial, institutional, and commercial sources. The results of the biogas resource assessment are used to estimate the potential production of renewable hydrogen from biogas as well as the fuel cell electric vehicles that the produced hydrogen might support.

  16. Biogas: Production and utilization

    NASA Astrophysics Data System (ADS)

    Price, E. C.; Cheremisinoff, P. N.

    Among the aspects of biogas production and utilization covered are: (1) the microbiology and biochemistry of the acid and methane production stages in the anaerobic process; (2) factors affecting the process, such as temperature, acidity and alkalinity, nutrients, and cations; (3) denitrification processes and systems; and (4) the process kinetics of suspended growth systems, packed columns, and fluidized beds. Also considered are such issues in the application of this technology as the digestion of municipal treatment plant sludges, animal wastes, food processing wastes and energy crops. Attention is in addition given to anaerobic digester design, offgas measurement of anaerobic digesters, and sludge treatment through soil conditioning and composting.

  17. Plant-Parasitic Nematodes in Maine Agricultural Soils

    PubMed Central

    Huettel, W N.; Francl, L. J.; Henn, A.; Bourgoin, T.

    1990-01-01

    In a survey of plant-parasitic nematodes associated with agricultural crops in nine Maine counties, 744 soil samples from 26 potential host plants were analyzed between November 1987 and January 1989. The most commonly encountered nematode genus was Pratylenchus, occurring in 85% of the samples from most crops, except blueberries and onions. Pratylenchus penetrans and P. crenatus were found commonly as species mixtures, with P. penetrans composing 40-80% of the mixture. Meloidogyne hapla was encountered in 16% of the samples in four counties, generally in potato rotations. Other nematodes encountered were Aphelenchoides spp., Criconemella curvature, Ditylenchus spp., Helicotylenchus pseudorobustus, H. digonicus, Heterodera trifolii, Paratylenchus projectus, Trichodorus spp., Tylenchorhynchus maximus, and Xiphinema americanum. Potato fields were the most heavily sampled and thus weighted the statewide results. PMID:19287791

  18. Role of transgenic plants in agriculture and biopharming.

    PubMed

    Ahmad, Parvaiz; Ashraf, Muhammad; Younis, Muhammad; Hu, Xiangyang; Kumar, Ashwani; Akram, Nudrat Aisha; Al-Qurainy, F

    2012-01-01

    At present, environmental degradation and the consistently growing population are two main problems on the planet earth. Fulfilling the needs of this growing population is quite difficult from the limited arable land available on the globe. Although there are legal, social and political barriers to the utilization of biotechnology, advances in this field have substantially improved agriculture and human life to a great extent. One of the vital tools of biotechnology is genetic engineering (GE) which is used to modify plants, animals and microorganisms according to desired needs. In fact, genetic engineering facilitates the transfer of desired characteristics into other plants which is not possible through conventional plant breeding. A variety of crops have been engineered for enhanced resistance to a multitude of stresses such as herbicides, insecticides, viruses and a combination of biotic and abiotic stresses in different crops including rice, mustard, maize, potato, tomato, etc. Apart from the use of GE in agriculture, it is being extensively employed to modify the plants for enhanced production of vaccines, hormones, etc. Vaccines against certain diseases are certainly available in the market, but most of them are very costly. Developing countries cannot afford the disease control through such cost-intensive vaccines. Alternatively, efforts are being made to produce edible vaccines which are cheap and have many advantages over the commercialized vaccines. Transgenic plants generated for this purpose are capable of expressing recombinant proteins including viral and bacterial antigens and antibodies. Common food plants like banana, tomato, rice, carrot, etc. have been used to produce vaccines against certain diseases like hepatitis B, cholera, HIV, etc. Thus, the up- and down-regulation of desired genes which are used for the modification of plants have a marked role in the improvement of genetic crops. In this review, we have comprehensively discussed the role

  19. Decolourisation of palm oil mill biogas plant wastewater using Poly-Diallyldimethyl Ammonium Chloride (polyDADMAC) and other chemical coagulants

    NASA Astrophysics Data System (ADS)

    Zahrim, A. Y.; Dexter, Z. D.

    2016-06-01

    Palm oil mill effluent was expected as a future source of renewable biogas. Nevertheless,colours in palm oil mill biogas plant wastewater (POMBPW) causes negative perception among the public and the wastewater is difficult to be treated biologically. In this study, the performance of various chemical coagulants i.e., calcium lactate, magnesium hydroxide, ferric chloride, aluminium chlorohydrate i.e. CK-800, CK-1000, and polyDADMAC, forPOMBPW colour removal were investigated. PolyDADMAC (1,000 mg/L) shows best colour removal (∼48%). The main coagulation process with polyDADMACcould be due to charge neutralization-bridging mechanism. The zeta potential analysis supports the finding where the value became positive as the dosage increases. The addition of polyDADMAC has increased the conductivity of the treated wastewater up to 9.22%; however, the final pH is maintained (8.0-8.3). It can be deduced that polyDADMAC has potential to treat POMBPW at low dosage.

  20. Life Cycle Environmental Impacts of Electricity from Biogas Produced by Anaerobic Digestion.

    PubMed

    Fusi, Alessandra; Bacenetti, Jacopo; Fiala, Marco; Azapagic, Adisa

    2016-01-01

    The aim of this study was to evaluate life cycle environmental impacts associated with the generation of electricity from biogas produced by the anaerobic digestion (AD) of agricultural products and waste. Five real plants in Italy were considered, using maize silage, slurry, and tomato waste as feedstocks and cogenerating electricity and heat; the latter is not utilized. The results suggest that maize silage and the operation of anaerobic digesters, including open storage of digestate, are the main contributors to the impacts of biogas electricity. The system that uses animal slurry is the best option, except for the marine and terrestrial ecotoxicity. The results also suggest that it is environmentally better to have smaller plants using slurry and waste rather than bigger installations, which require maize silage to operate efficiently. Electricity from biogas is environmentally more sustainable than grid electricity for seven out of 11 impacts considered. However, in comparison with natural gas, biogas electricity is worse for seven out of 11 impacts. It also has mostly higher impacts than other renewables, with a few exceptions, notably solar photovoltaics. Thus, for the AD systems and mesophilic operating conditions considered in this study, biogas electricity can help reduce greenhouse gas (GHG) emissions relative to a fossil-intensive electricity mix; however, some other impacts increase. If mitigation of climate change is the main aim, other renewables have a greater potential to reduce GHG emissions. If, in addition to this, other impacts are considered, then hydro, wind, and geothermal power are better alternatives to biogas electricity. However, utilization of heat would improve significantly its environmental sustainability, particularly global warming potential, summer smog, and the depletion of abiotic resources and the ozone layer. Further improvements can be achieved by banning open digestate storage to prevent methane emissions and regulating

  1. Life Cycle Environmental Impacts of Electricity from Biogas Produced by Anaerobic Digestion

    PubMed Central

    Fusi, Alessandra; Bacenetti, Jacopo; Fiala, Marco; Azapagic, Adisa

    2016-01-01

    The aim of this study was to evaluate life cycle environmental impacts associated with the generation of electricity from biogas produced by the anaerobic digestion (AD) of agricultural products and waste. Five real plants in Italy were considered, using maize silage, slurry, and tomato waste as feedstocks and cogenerating electricity and heat; the latter is not utilized. The results suggest that maize silage and the operation of anaerobic digesters, including open storage of digestate, are the main contributors to the impacts of biogas electricity. The system that uses animal slurry is the best option, except for the marine and terrestrial ecotoxicity. The results also suggest that it is environmentally better to have smaller plants using slurry and waste rather than bigger installations, which require maize silage to operate efficiently. Electricity from biogas is environmentally more sustainable than grid electricity for seven out of 11 impacts considered. However, in comparison with natural gas, biogas electricity is worse for seven out of 11 impacts. It also has mostly higher impacts than other renewables, with a few exceptions, notably solar photovoltaics. Thus, for the AD systems and mesophilic operating conditions considered in this study, biogas electricity can help reduce greenhouse gas (GHG) emissions relative to a fossil-intensive electricity mix; however, some other impacts increase. If mitigation of climate change is the main aim, other renewables have a greater potential to reduce GHG emissions. If, in addition to this, other impacts are considered, then hydro, wind, and geothermal power are better alternatives to biogas electricity. However, utilization of heat would improve significantly its environmental sustainability, particularly global warming potential, summer smog, and the depletion of abiotic resources and the ozone layer. Further improvements can be achieved by banning open digestate storage to prevent methane emissions and regulating

  2. Agricultural plants and soil as a reservoir for Pseudomonas aeruginosa.

    PubMed

    Green, S K; Schroth, M N; Cho, J J; Kominos, S K; Vitanza-jack, V B

    1974-12-01

    Pseudomonas aeruginosa was detected in 24% of the soil samples but in only 0.13% of the vegetable samples from various agricultural areas of California. The distribution of pyocin types of soil and vegetable isolates was similar to that of clinical strains, and three of the soil isolates were resistant to carbenicillin. Pseudomonas aeruginosa multiplied in lettuce and bean under conditions of high temperature and high relative humidity (27 C and 80-95% relative humidity) but declined when the temperature and humidity were lowered (16 C, 55-75% relative humidity). The results suggest that soil is a reservior for P. aeruginosa and that the bacterium has the capacity to colonize plants during favorable conditions of temperature and moisture. PMID:4217591

  3. Agricultural Plants and Soil as a Reservoir for Pseudomonas aeruginosa

    PubMed Central

    Green, Sylvia K.; Schroth, Milton N.; Cho, John J.; Kominos, Spyros D.; Vitanza-Jack, Vilma B.

    1974-01-01

    Pseudomonas aeruginosa was detected in 24% of the soil samples but in only 0.13% of the vegetable samples from various agricultural areas of California. The distribution of pyocin types of soil and vegetable isolates was similar to that of clinical strains, and three of the soil isolates were resistant to carbenicillin. Pseudomonas aeruginosa multiplied in lettuce and bean under conditions of high temperature and high relative humidity (27 C and 80-95% relative humidity) but declined when the temperature and humidity were lowered (16 C, 55-75% relative humidity). The results suggest that soil is a reservior for P. aeruginosa and that the bacterium has the capacity to colonize plants during favorable conditions of temperature and moisture. PMID:4217591

  4. Anaerobic digestion of agricultural and other substrates--implications for greenhouse gas emissions.

    PubMed

    Pucker, J; Jungmeier, G; Siegl, S; Pötsch, E M

    2013-06-01

    The greenhouse gas (GHG) emissions, expressed in carbon dioxide equivalents (CO2-eq), of different Austrian biogas systems were analyzed and evaluated using life-cycle assessment (LCA) as part of a national project. Six commercial biogas plants were investigated and the analysis included the complete process chain: viz., the production and collection of substrates, the fermentation of the substrates in the biogas plant, the upgrading of biogas to biomethane (if applicable) and the use of the biogas or biomethane for heat and electricity or as transportation fuel. Furthermore, the LCA included the GHG emissions of construction, operation and dismantling of the major components involved in the process chain, as well as the use of by-products (e.g. fermentation residues used as fertilizers). All of the biogas systems reduced GHG emissions (in CO2-eq) compared with fossil reference systems. The potential for GHG reduction of the individual biogas systems varied between 60% and 100%. Type of feedstock and its reference use, agricultural practices, coverage of storage tanks for fermentation residues, methane leakage at the combined heat and power plant unit and the proportion of energy used as heat were identified as key factors influencing the GHG emissions of anaerobic digestion processes. PMID:23739470

  5. Detailed analysis of metagenome datasets obtained from biogas-producing microbial communities residing in biogas reactors does not indicate the presence of putative pathogenic microorganisms

    PubMed Central

    2013-01-01

    Background In recent years biogas plants in Germany have been supposed to be involved in amplification and dissemination of pathogenic bacteria causing severe infections in humans and animals. In particular, biogas plants are discussed to contribute to the spreading of Escherichia coli infections in humans or chronic botulism in cattle caused by Clostridium botulinum. Metagenome datasets of microbial communities from an agricultural biogas plant as well as from anaerobic lab-scale digesters operating at different temperatures and conditions were analyzed for the presence of putative pathogenic bacteria and virulence determinants by various bioinformatic approaches. Results All datasets featured a low abundance of reads that were taxonomically assigned to the genus Escherichia or further selected genera comprising pathogenic species. Higher numbers of reads were taxonomically assigned to the genus Clostridium. However, only very few sequences were predicted to originate from pathogenic clostridial species. Moreover, mapping of metagenome reads to complete genome sequences of selected pathogenic bacteria revealed that not the pathogenic species itself, but only species that are more or less related to pathogenic ones are present in the fermentation samples analyzed. Likewise, known virulence determinants could hardly be detected. Only a marginal number of reads showed similarity to sequences described in the Microbial Virulence Database MvirDB such as those encoding protein toxins, virulence proteins or antibiotic resistance determinants. Conclusions Findings of this first study of metagenomic sequence reads of biogas producing microbial communities suggest that the risk of dissemination of pathogenic bacteria by application of digestates from biogas fermentations as fertilizers is low, because obtained results do not indicate the presence of putative pathogenic microorganisms in the samples analyzed. PMID:23557021

  6. Sludge storage lagoon biogas recovery and use

    SciTech Connect

    Muller, D.; Norville, C. )

    1991-07-01

    The City of Memphis has two wastewater treatment plants. The SWTP employs two large anaerobic digestion sludge lagoons as part of the overall sludge treatment system. Although these lagoons are effective in concentrating and digesting sludge, they can generate offensive odors. The SWTP uses aerobic digesters to partially stabilize the sludge and help reduce objectionable odors before it enters the lagoons. The anaerobic digestion of sludge in the lagoons results in the dispersion of a large quantity of biogas into the atmosphere. The City realized that if the lagoons could be covered, the odor problem could be resolved, and at the same, time, biogas could be recovered and utilized as a source of energy. In 1987, the City commissioned ADI International to conduct a feasibility study to evaluate alternative methods of covering the lagoons and recovering and utilizing the biogas. The study recommended that the project be developed in two phases: (1) recovery of the biogas and (2) utilization of the biogas. Phase 1 consists of covering the two lagoons with an insulated membrane to control odor and temperature and collect the biogas. Phase 1 was found to be economically feasible and offered a unique opportunity for the City to save substantial operating costs at the treatment facility. The Memphis biogas recovery project is the only application in the world where a membrane cover has been used on a municipal wastewater sludge lagoon. It is also the largest lagoon cover system in the world.

  7. Agricultural Application of Higher Plants for Their Antimicrobial Potentials in China

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This chapter describes published research into Chinese medicinal and aromatic plants as sources of new crop protectants. Literature was reviewed based on our personal experience, reported antimicrobial activity against plant pathogens, novel chemical structures, and potential for agricultural utiliz...

  8. Revising and Updating the Plant Science Components of the Connecticut Vocational Agriculture Curriculum.

    ERIC Educational Resources Information Center

    Connecticut Univ., Storrs. Dept. of Educational Leadership.

    This curriculum guide provides the plant science components of the vocational agriculture curriculum for Regional Vocational Agriculture Centers. The curriculum is divided into exploratory units for students in the 9th and 10th grades and specialized units for students in grades 11 and 12. The five exploratory units are: agricultural pest control;…

  9. Gasification of agricultural residues in a demonstrative plant: corn cobs.

    PubMed

    Biagini, Enrico; Barontini, Federica; Tognotti, Leonardo

    2014-12-01

    Biomass gasification couples the high power efficiency with the possibility of valuably using the byproducts heat and biochar. The use of agricultural wastes instead of woody feedstock extends the seasonal availability of biomasses. The downdraft type is the most used reactor but has narrow ranges of feedstock specifications (above all on moisture and particle size distribution), so tests on a demonstrative scale are conducted to prove the versatility of the gasifier. Measurements on pressure drops, syngas flow rate and composition are studied to assess the feasibility of such operations with corn cobs. Material and energy balances, and performance indexes are compared for the four tests carried out under different biomass loads (66-85 kg/h). A good operability of the plant and interesting results are obtained (gas specific production of 2 m3/kg, gas heating value 5.6-5.8 MJ/m3, cold gas efficiency in the range 66-68%, potential net power efficiency 21.1-21.6%). PMID:25299486

  10. The good, the bad or the ugly: Microbial biomass of biogas residues as a contributor to soil carbon cycle

    NASA Astrophysics Data System (ADS)

    Coban, H.; Miltner, A.; Kaestner, M.

    2013-12-01

    Loss of soil organic matter is a recent problem in soils all over the world. This can be related to enhanced mineralization of the soil organic matter due to land use change, which is a source of anthropogenic carbon dioxide increase. For example, the carbon input from plant residues is reduced because of the increased cultivation of bioenergy crops. In order to avoid soil degradation, application of biogas residues is a common practice in such areas. Biogas residues are side products of biogas production and contain microbial biomass. Application of these residues as soil additive influences the soil microorganisms as well as the carbon cycle. We study this effect by incubating 13C-labeled biogas residues in an arable soil from the Static Fertilization Experiment in Bad Lauchstaedt, Germany. Labeled residues were produced via labeling of active microbial biomass by addition of KH13CO3 to biogas reactors. High enrichment in the various phospholipid fatty acids proved the successful labeling of the biomass. The labeled biogas residues are being long-term incubated in the soil. During incubation, we monitor the fate of the carbon by analyzing the label in phospholipid fatty acids, amino acids as well as carbon dioxide. This allows us to trace the fate of the biogas residues-derived C in soil and to quantify the effect on the transformation of the natural soil organic matter (e.g. negative effects such as priming effects). Also, microbial community dynamics will be determined using molecular biology tools such as denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (Q-PCR). In order to prevent potentially negative effects, various additives such as charred biomaterials, clays and chopped bark will be tested to improve the carbon storage in soil. In conclusion, this study investigates the fate and impact of biogas residues used as a soil additive on the soil microbial community and amount of soil organic matter. It is aimed to understand and

  11. Metagenome and metaproteome analyses of microbial communities in mesophilic biogas-producing anaerobic batch fermentations indicate concerted plant carbohydrate degradation.

    PubMed

    Hanreich, Angelika; Schimpf, Ulrike; Zakrzewski, Martha; Schlüter, Andreas; Benndorf, Dirk; Heyer, Robert; Rapp, Erdmann; Pühler, Alfred; Reichl, Udo; Klocke, Michael

    2013-07-01

    Microbial communities in biogas batch fermentations, using straw and hay as co-substrates, were analyzed at the gene and protein level by metagenomic and metaproteomic approaches. The analysis of metagenomic data revealed that the Clostridiales and Bacteroidales orders were prevalent in the community. However, the number of sequences assigned to the Clostridiales order decreased during fermentation, whereas the number of sequences assigned to the Bacteroidales order increased. In addition, changes at the functional level were monitored and the metaproteomic analyses detected transporter proteins and flagellins, which were expressed mainly by members of the Bacteroidetes and Firmicutes phyla. A high number of sugar transporters, expressed by members of the Bacteroidetes, proved their potential to take up various glycans efficiently. Metagenome data also showed that methanogenic organisms represented less than 4% of the community, while 20-30% of the identified proteins were of archeal origin. These data suggested that methanogens were disproportionally active. In conclusion, the community studied was capable of digesting the recalcitrant co-substrate. Members of the Firmicutes phylum seemed to be the main degraders of cellulose, even though expression of only a few glycoside hydrolases was detected. The Bacteroidetes phylum expressed a high number of sugar transporters and seemed to specialize in the digestion of other polysaccharides. Finally, it was found that key enzymes of methanogenesis were expressed in high quantities, indicating the high metabolic activity of methanogens, although they only represented a minor group within the microbial community. PMID:23694815

  12. Biogas electricity -- The Pura village case study

    SciTech Connect

    Rajabapaiah, P.; Jayakumar, S.; Reddy, A.K.N.

    1993-12-31

    A potentially useful decentralized source of energy is biogas, which is an approximately 60:40 mixture of methane (CH{sub 4}) and carbon dioxide (CO{sub 2}), produced by the anaerobic fermentation of cellulosic biomass materials such as bovine wastes. Since 1987, the traditional system of obtaining water, illumination, and fertilizer in Pura village in south India has been replaced with a community biogas plant electricity-generation system. The technical, managerial, and economical aspects of this system are the subject manner of the present paper. Various subsystems are described, and the problems of operation and maintenance under field conditions are also discussed. A comparison of Pura`s present community biogas system with its traditional means for obtaining water, illumination, and fertilizer shows that the households are winners on all counts, having obtained such benefits as improved hygiene and convenience at relatively low cost. The Pura community biogas plant is held together and sustained by the convergence of individual and collective interests. Noncooperation with the community biogas plant results in a heavy individual price (access to water and light being cut off by the village), which is too great a personal loss to compensate for the minor advantages of noncooperation and noncontribution to collective interests.

  13. Potential alternative fuel sources for agricultural crops and plant components

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The changing landscape of agricultural production is placing unprecedented demands on farmers as they face increasing global competition and greater natural resource conservation challenges. However, shrinking profit margins due to increasing input costs, particularly of fuel and fertilizer, can res...

  14. Improved detection of extended spectrum beta-lactamase (ESBL)-producing Escherichia coli in input and output samples of German biogas plants by a selective pre-enrichment procedure.

    PubMed

    Schauss, Thorsten; Glaeser, Stefanie P; Gütschow, Alexandra; Dott, Wolfgang; Kämpfer, Peter

    2015-01-01

    The presence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli was investigated in input (manure from livestock husbandry) and output samples of six German biogas plants in 2012 (one sampling per biogas plant) and two German biogas plants investigated in an annual cycle four times in 2013/2014. ESBL-producing Escherichia coli were cultured by direct plating on CHROMagar ESBL from input samples in the range of 100 to 104 colony forming units (CFU) per g dry weight but not from output sample. This initially indicated a complete elimination of ESBL-producing E. coli by the biogas plant process. Detected non target bacteria were assigned to the genera Acinetobacter, Pseudomonas, Bordetella, Achromobacter, Castellaniella, and Ochrobactrum. A selective pre-enrichment procedure increased the detection efficiency of ESBL-producing E. coli in input samples and enabled the detection in five of eight analyzed output samples. In total 119 ESBL-producing E. coli were isolated from input and 46 from output samples. Most of the E. coli isolates carried CTX-M-type and/or TEM-type beta lactamases (94%), few SHV-type beta lactamase (6%). Sixty-four blaCTX-M genes were characterized more detailed and assigned mainly to CTX-M-groups 1 (85%) and 9 (13%), and one to group 2. Phylogenetic grouping of 80 E. coli isolates showed that most were assigned to group A (71%) and B1 (27%), only one to group D (2%). Genomic fingerprinting and multilocus sequence typing (MLST) showed a high clonal diversity with 41 BOX-types and 19 ST-types. The two most common ST-types were ST410 and ST1210. Antimicrobial susceptibility testing of 46 selected ESBL-producing E. coli revealed that several isolates were additionally resistant to other veterinary relevant antibiotics and some grew on CHROMagar STEC but shiga-like toxine (SLT) genes were not detected. Resistance to carbapenems was not detected. In summary the study showed for the first time the presence of ESBL-producing E. coli in

  15. Improved Detection of Extended Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in Input and Output Samples of German Biogas Plants by a Selective Pre-Enrichment Procedure

    PubMed Central

    Schauss, Thorsten; Glaeser, Stefanie P.; Gütschow, Alexandra; Dott, Wolfgang; Kämpfer, Peter

    2015-01-01

    The presence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli was investigated in input (manure from livestock husbandry) and output samples of six German biogas plants in 2012 (one sampling per biogas plant) and two German biogas plants investigated in an annual cycle four times in 2013/2014. ESBL-producing Escherichia coli were cultured by direct plating on CHROMagar ESBL from input samples in the range of 100 to 104 colony forming units (CFU) per g dry weight but not from output sample. This initially indicated a complete elimination of ESBL-producing E. coli by the biogas plant process. Detected non target bacteria were assigned to the genera Acinetobacter, Pseudomonas, Bordetella, Achromobacter, Castellaniella, and Ochrobactrum. A selective pre-enrichment procedure increased the detection efficiency of ESBL-producing E. coli in input samples and enabled the detection in five of eight analyzed output samples. In total 119 ESBL-producing E. coli were isolated from input and 46 from output samples. Most of the E. coli isolates carried CTX-M-type and/or TEM-type beta lactamases (94%), few SHV-type beta lactamase (6%). Sixty-four blaCTX-M genes were characterized more detailed and assigned mainly to CTX-M-groups 1 (85%) and 9 (13%), and one to group 2. Phylogenetic grouping of 80 E. coli isolates showed that most were assigned to group A (71%) and B1 (27%), only one to group D (2%). Genomic fingerprinting and multilocus sequence typing (MLST) showed a high clonal diversity with 41 BOX-types and 19 ST-types. The two most common ST-types were ST410 and ST1210. Antimicrobial susceptibility testing of 46 selected ESBL-producing E. coli revealed that several isolates were additionally resistant to other veterinary relevant antibiotics and some grew on CHROMagar STEC but shiga-like toxine (SLT) genes were not detected. Resistance to carbapenems was not detected. In summary the study showed for the first time the presence of ESBL-producing E. coli in

  16. Using microbial community interactions within plant microbiomes to advance an evergreen agricultural revolution

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Innovative plant breeding and technology transfer fostered the Green Revolution, which transformed agriculture worldwide by increasing grain yields in developing countries. The Green Revolution temporarily alleviated world hunger, but also reduced biodiversity, nutrient cycling, and carbon sequestr...

  17. EX SITU PRESERVATION OF PLANT GENETIC RESOURCES FOR FOOD AND AGRICULTURE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The US National Plant Germplasm System, (NPGS) a group within the Agricultural Research Service of the United States Department of Agriculture, is comprised of over 20 field stations spread throughout the US and is responsible for the preservation and distribution of genetic resources important for ...

  18. Relationship between humanity and plant natural resources – in the context of food and agriculture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Agriculture, the domestication, culture, and management of plants and animals, has led to profound social changes in human evolution and development; it can be considered as the basis for civilization. Roughly 12,000 years ago agriculture appeared independently in several parts of the world. A natur...

  19. Plant growth-promoting bacteria as inoculants in agricultural soils.

    PubMed

    Souza, Rocheli de; Ambrosini, Adriana; Passaglia, Luciane M P

    2015-12-01

    Plant-microbe interactions in the rhizosphere are the determinants of plant health, productivity and soil fertility. Plant growth-promoting bacteria (PGPB) are bacteria that can enhance plant growth and protect plants from disease and abiotic stresses through a wide variety of mechanisms; those that establish close associations with plants, such as the endophytes, could be more successful in plant growth promotion. Several important bacterial characteristics, such as biological nitrogen fixation, phosphate solubilization, ACC deaminase activity, and production of siderophores and phytohormones, can be assessed as plant growth promotion (PGP) traits. Bacterial inoculants can contribute to increase agronomic efficiency by reducing production costs and environmental pollution, once the use of chemical fertilizers can be reduced or eliminated if the inoculants are efficient. For bacterial inoculants to obtain success in improving plant growth and productivity, several processes involved can influence the efficiency of inoculation, as for example the exudation by plant roots, the bacterial colonization in the roots, and soil health. This review presents an overview of the importance of soil-plant-microbe interactions to the development of efficient inoculants, once PGPB are extensively studied microorganisms, representing a very diverse group of easily accessible beneficial bacteria. PMID:26537605

  20. Plant growth-promoting bacteria as inoculants in agricultural soils

    PubMed Central

    de Souza, Rocheli; Ambrosini, Adriana; Passaglia, Luciane M.P.

    2015-01-01

    Abstract Plant-microbe interactions in the rhizosphere are the determinants of plant health, productivity and soil fertility. Plant growth-promoting bacteria (PGPB) are bacteria that can enhance plant growth and protect plants from disease and abiotic stresses through a wide variety of mechanisms; those that establish close associations with plants, such as the endophytes, could be more successful in plant growth promotion. Several important bacterial characteristics, such as biological nitrogen fixation, phosphate solubilization, ACC deaminase activity, and production of siderophores and phytohormones, can be assessed as plant growth promotion (PGP) traits. Bacterial inoculants can contribute to increase agronomic efficiency by reducing production costs and environmental pollution, once the use of chemical fertilizers can be reduced or eliminated if the inoculants are efficient. For bacterial inoculants to obtain success in improving plant growth and productivity, several processes involved can influence the efficiency of inoculation, as for example the exudation by plant roots, the bacterial colonization in the roots, and soil health. This review presents an overview of the importance of soil-plant-microbe interactions to the development of efficient inoculants, once PGPB are extensively studied microorganisms, representing a very diverse group of easily accessible beneficial bacteria. PMID:26537605

  1. Energy Efficiency of Biogas Produced from Different Biomass Sources

    NASA Astrophysics Data System (ADS)

    Begum, Shahida; Nazri, A. H.

    2013-06-01

    Malaysia has different sources of biomass like palm oil waste, agricultural waste, cow dung, sewage waste and landfill sites, which can be used to produce biogas and as a source of energy. Depending on the type of biomass, the biogas produced can have different calorific value. At the same time the energy, being used to produce biogas is dependent on transportation distance, means of transportation, conversion techniques and for handling of raw materials and digested residues. An energy systems analysis approach based on literature is applied to calculate the energy efficiency of biogas produced from biomass. Basically, the methodology is comprised of collecting data, proposing locations and estimating the energy input needed to produce biogas and output obtained from the generated biogas. The study showed that palm oil and municipal solid waste is two potential sources of biomass. The energy efficiency of biogas produced from palm oil residues and municipal solid wastes is 1.70 and 3.33 respectively. Municipal solid wastes have the higher energy efficiency due to less transportation distance and electricity consumption. Despite the inherent uncertainties in the calculations, it can be concluded that the energy potential to use biomass for biogas production is a promising alternative.

  2. Agricultural Waste.

    PubMed

    Shu, Huajie; Zhang, Panpan; Chang, Chein-Chi; Wang, Renqing; Zhang, Shuping

    2015-10-01

    The management and disposal of agricultural waste are drawn more and more attention because of the increasing yields and negative effects on the environment. However, proper treatments such as converting abundant biomass wastes into biogas through anaerobic digestion technology, can not only avoid the negative impacts, but also convert waste into available resources. This review summarizes the studies of nearly two hundred scholars from the following four aspects: the characterization, reuse, treatment, and management of agricultural waste. PMID:26420088

  3. Chemosensitization of plant pathogenic fungi to agricultural fungicides

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Continual use of fungicides in agriculture often results in development of resistance in pathogens. A new strategy to aid in overcoming or minimizing this problem is enhancement of pathogen sensitivity to fungicides by chemosensitization. This involves combining fungicides with certain non-fungicida...

  4. Plant breeding for harmony between agriculture and the environment

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Crop improvements made since the 1950’s coupled with inexpensive agronomic inputs (fertilizers, herbicides, etc.) have resulted in agricultural production that has kept pace with population growth. Breeding programs primarily focus on improving a crop’s environmental adaptability and biotic stress t...

  5. Landscape context and plant community composition in grazed agricultural systems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Temperate humid grazinglands are an important component of the landscape of the northeastern Unites States. Grass-based agriculture is a major contributor to the economy of this region, but little is known about the basic ecology of these grazinglands. During an eight-year survey of 28 farms across ...

  6. Educational Plant Survey: Florida Agricultural and Mechanical University, April 1-5, 1991.

    ERIC Educational Resources Information Center

    Florida State Dept. of Education, Tallahassee.

    This publication is the report of findings of the educational plant survey for Florida Agricultural and Mechanical University conducted in April, 1991. Following a description of the survey team and a preface, Section I describes the statutory foundations, procedural policies, and cooperative processes for the educational plant survey. Section II…

  7. Accumulation of artificial radionuclides in agricultural plants in the area used for surface nuclear tests.

    PubMed

    Kozhakhanov, T E; Lukashenko, S N; Larionova, N V

    2014-11-01

    The paper reports on the study of artificial radionuclide accumulation in agricultural crops grown at the territory with high concentration of radionuclides, and first of all - with high concentration of transuranium elements. As a result of this work, peculiarities of accumulation and distribution of artificial radionuclides in the vegetative and generative organs of the studied plants have been revealed. Basic accumulation factors have been found for (137)Cs, (90)Sr, (239+240)Pu, and (241)Am in agricultural products. Accumulation factor dependence on type of planting was found for the investigated types of plants. It has been found that the vegetative organs accumulate radionuclides most of all. PMID:25128979

  8. Creating an agricultural world order: regional plant protection problems and international phytopathology, 1878-1939.

    PubMed

    Castonguay, Stéphane

    2010-01-01

    Beginning in 1878 with the International Phylloxera Convention of Berne, international conventions have sought to relieve national agricultural industries from two specific burdens. First, by defining phytosanitary practices to be enforced by national plant protection services, these conventions attempted to prevent the introduction of plant diseases and pests into national territories from which they were previously absent. Second, by standardizing these practices - especially through the design of a unique certificate of inspection - the conventions attempted to eliminate barriers such as quarantines affection international agricultural trade. The succession of phytopathological conventions seemed to epitomize the coalescence of an international community against agricultural pests. What actually coalesced was bio-geopolitics wherein plant pathologists and economic entomologists from North America and the British Empire questioned the so-called internationality of the environmental and economic specificities of continental European agriculture, embodied in "international" conventions. Although an international phenomenon, the dissemination of agricultural pests provided opportunities for cooperation on a strictly regional albeit transnational basis that pitted bio-geopolitical spaces against each other. This article retraces the formation of these spaces by analyzing the deliberations of committees and congresses that gathered to define an international agricultural order based on the means to prevent the spread of plant diseases and pests. PMID:20329355

  9. Current EU-27 technical potential of organic waste streams for biogas and energy production.

    PubMed

    Lorenz, Helge; Fischer, Peter; Schumacher, Britt; Adler, Philipp

    2013-11-01

    Anaerobic digestion of organic waste generated by households, businesses, agriculture, and industry is an important approach as method of waste treatment - especially with regard to its potential as an alternative energy source and its cost-effectiveness. Separate collection of biowaste from households or vegetal waste from public green spaces is already established in some EU-27 countries. The material recovery in composting plants is common for biowaste and vegetal waste. Brewery waste fractions generated by beer production are often used for animal feeding after a suitable preparation. Waste streams from paper industry generated by pulp and paper production such as black liquor or paper sludge are often highly contaminated with toxic substances. Recovery of chemicals and the use in thermal processes like incineration, pyrolysis, and gasification are typical utilization paths. The current utilization of organic waste from households and institutions (without agricultural waste) was investigated for EU-27 countries with Germany as an in-depth example. Besides of biowaste little is known about the suitability of waste streams from brewery and paper industry for anaerobic digestion. Therefore, an evaluation of the most important biogas process parameters for different substrates was carried out, in order to calculate the biogas utilization potential of these waste quantities. Furthermore, a calculation of biogas energy potentials was carried out for defined waste fractions which are most suitable for anaerobic digestion. Up to 1% of the primary energy demand can be covered by the calculated total biogas energy potential. By using a "best-practice-scenario" for separately collected biowaste, the coverage of primary energy demand may be increased above 2% for several countries. By using sector-specific waste streams, for example the German paper industry could cover up to 4.7% and the German brewery industry up to 71.2% of its total energy demand. PMID:23849753

  10. Drought Impacts on Ancient Maya Maize Agriculture Inferred from Isotopic Analyses of Plant Biomarkers

    NASA Astrophysics Data System (ADS)

    Douglas, P. M.; Pagani, M.; Eglinton, T. I.; Brenner, M.; Hodell, D. A.; Curtis, J. H.

    2013-05-01

    There is increasing evidence suggesting that a series of droughts in the Maya lowlands of southeastern Mexico and northern Central America coincided with the Terminal Classic decline of the Classic Maya civilization (ca. 1250 to 1000 years BP). However, there is little evidence directly linking climatic change and changes in human activities in this region. In this study we combine plant-wax hydrogen and carbon analyses in two lake sediment cores from the Yucatan and northern Guatemala to develop coupled records of hydroclimate variability and human-driven vegetation change and assess drought impacts on maize agriculture In the Maya lowlands plant-wax hydrogen isotope ratios (δD) are controlled by the isotopic composition of precipitation and evapotranspiration, and are highly sensitive to changes in aridity. In this low-elevation tropical environment plant-wax carbon isotope ratios (δ13C) are largely controlled by the relative abundance of C3 and C4 plants. The ancient Maya practiced widespread maize (C4) agriculture and strongly influenced regional C3-C4 vegetation dynamics. Under natural conditions C4 plant coverage and plant-wax δD would tend to co-vary positively since C4 plants are well adapted for dry conditions. Under ancient Maya land-use, however, this relationship is likely to be decoupled, since drought would have disrupted C4 agriculture. Combined analyses of plant-wax δD and δ13C from two lake sediment cores in the Maya lowlands indicate co-evolving changes in hydroclimate and C4 plant coverage over the past 4000 years. Compound-specific radiocarbon analyses of plant-waxes provide independent chronologies for these plant-wax stable isotope records, and plant-wax δD records developed using these chronologies agree closely with other regional records of hydroclimate change. Trends in plant-wax δD and δ13C diverge following ca. 3500 years BP, around the onset of widespread ancient Maya agriculture. After this time high plant-wax δD values tend

  11. Management of root-knot nematode in tomato Lycopersicon esculentum, Mill, with biogas slurry.

    PubMed

    Jothi, G; Pugalendhi, S; Poornima, K; Rajendran, G

    2003-09-01

    The effect of biogas slurry application on the severity of root-knot nematode, Meloidogyne incognita, attack on tomato cv. Co-1, was tested in the green house with two levels of biogas slurry: 5% and 10% (w/w) added to soil. Both the number (3 fruits/plant) and fruit yield (35.2 g/plant) of tomato increased significantly with 10% (w/w) biogas slurry. The plants amended with biogas slurry put up more vegetative growth and tended to flower and fruit much earlier than did those of the control. The nematode population in the soil decreased thus decreasing the severity of nematode attack. PMID:12699936

  12. Biogas Production Modelling: A Control System Engineering Approach

    NASA Astrophysics Data System (ADS)

    Stollenwerk, D.; Rieke, C.; Dahmen, M.; Pieper, M.

    2016-03-01

    Due to the Renewable Energy Act, in Germany it is planned to increase the amount of renewable energy carriers up to 60%. One of the main problems is the fluctuating supply of wind and solar energy. Here biogas plants provide a solution, because a demand-driven supply is possible. Before running such a plant, it is necessary to simulate and optimize the process feeding strategy. Current simulation models are either very detailed like the ADM 1, which leads to very long optimization runtimes or not accurate enough to handle the biogas production kinetics. Therefore this paper provides a new model of a biogas plant, which is easy to parametrize but also has the needed accuracy for the output prediction. It is based on the control system approach of system identification and validated with laboratory results of a real biogas production testing facility.

  13. Plant-microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture.

    PubMed

    Berg, Gabriele

    2009-08-01

    Plant-associated microorganisms fulfill important functions for plant growth and health. Direct plant growth promotion by microbes is based on improved nutrient acquisition and hormonal stimulation. Diverse mechanisms are involved in the suppression of plant pathogens, which is often indirectly connected with plant growth. Whereas members of the bacterial genera Azospirillum and Rhizobium are well-studied examples for plant growth promotion, Bacillus, Pseudomonas, Serratia, Stenotrophomonas, and Streptomyces and the fungal genera Ampelomyces, Coniothyrium, and Trichoderma are model organisms to demonstrate influence on plant health. Based on these beneficial plant-microbe interactions, it is possible to develop microbial inoculants for use in agricultural biotechnology. Dependent on their mode of action and effects, these products can be used as biofertilizers, plant strengtheners, phytostimulators, and biopesticides. There is a strong growing market for microbial inoculants worldwide with an annual growth rate of approximately 10%. The use of genomic technologies leads to products with more predictable and consistent effects. The future success of the biological control industry will benefit from interdisciplinary research, e.g., on mass production, formulation, interactions, and signaling with the environment, as well as on innovative business management, product marketing, and education. Altogether, the use of microorganisms and the exploitation of beneficial plant-microbe interactions offer promising and environmentally friendly strategies for conventional and organic agriculture worldwide. PMID:19568745

  14. Analysis of problems with dry fermentation process for biogas production

    NASA Astrophysics Data System (ADS)

    Pilát, Peter; Patsch, Marek; Jandačka, Jozef

    2012-04-01

    The technology of dry anaerobic fermentation is still meeting with some scepticism, and therefore in most biogas plants are used wet fermentation technology. Fermentation process would be not complete without an optimal controlled condition: dry matter content, density, pH, and in particular the reaction temperature. If is distrust of dry fermentation eligible it was on the workplace of the Department of Power Engineering at University of Zilina built an experimental small-scale biogas station that allows analysis of optimal parameters of the dry anaerobic fermentation, in particular, however, affect the reaction temperature on yield and quality of biogas.

  15. Biogas Production on Demand Regulated by Butyric Acid Addition

    NASA Astrophysics Data System (ADS)

    Kasper, K.; Schiffels, J.; Krafft, S.; Kuperjans, I.; Elbers, G.; Selmer, T.

    2016-03-01

    Investigating effects of volatile fatty acids on the biogas process it was observed that butyric acid can be used for transient stimulation of the methane production in biogas plants operating with low energy substrates like cattle manure. Upon addition of butyrate the methane output of the reactors doubled within 24 h and reached almost 3-times higher methane yields within 3-4 days. Butyrate was quantitatively eliminated and the reactors returned to the original productivity state within 3 days when application of butyrate was stopped. The opportunity to use butyrate feeding for increased biogas production on demand is discussed.

  16. Characterisation and treatment of VOCs in process water from upgrading facilities for compressed biogas (CBG).

    PubMed

    Nilsson Påledal, S; Arrhenius, K; Moestedt, J; Engelbrektsson, J; Stensen, K

    2016-02-01

    Compression and upgrading of biogas to vehicle fuel generates process water, which to varying degrees contains volatile organic compounds (VOCs) originating from the biogas. The compostion of this process water has not yet been studied and scientifically published and there is currently an uncertainty regarding content of VOCs and how the process water should be managed to minimise the impact on health and the environment. The aim of the study was to give an overview about general levels of VOCs in the process water. Characterisation of process water from amine and water scrubbers at plants digesting waste, sewage sludge or agricultural residues showed that both the average concentration and composition of particular VOCs varied depending on the substrate used at the biogas plant, but the divergence was high and the differences for total concentrations from the different substrate groups were only significant for samples from plants using waste compared to residues from agriculture. The characterisation also showed that the content of VOCs varied greatly between different sampling points for same main substrate and between sampling occasions at the same sampling point, indicating that site-specific conditions are important for the results which also indicates that a number of analyses at different times are required in order to make an more exact characterisation with low uncertainty. Inhibition of VOCs in the anaerobic digestion (AD) process was studied in biomethane potential tests, but no inhibition was observed during addition of synthetic process water at concentrations of 11.6 mg and 238 mg VOC/L. PMID:26694791

  17. Durable strategies to deploy plant resistance in agricultural landscapes.

    PubMed

    Fabre, Frédéric; Rousseau, Elsa; Mailleret, Ludovic; Moury, Benoit

    2012-03-01

    The deployment of resistant crops often leads to the emergence of resistance-breaking pathogens that suppress the yield benefit provided by the resistance. Here, we theoretically explored how farmers' main leverages (resistant cultivar choice, resistance deployment strategy, landscape planning and cultural practices) can be best combined to achieve resistance durability while minimizing yield losses as a result of plant viruses. Assuming a gene-for-gene type of interaction, virus epidemics are modelled in a landscape composed of a mosaic of resistant and susceptible fields, subjected to seasonality, and a reservoir hosting viruses year-round. The model links the genetic and the epidemiological processes, shaping at nested scales the demogenetic dynamics of viruses. The choice of the resistance gene (characterized by the equilibrium frequency of the resistance-breaking virus at mutation-selection balance in a susceptible plant) is the most influential leverage of action. Our results showed that optimal strategies of resistance deployment range from 'mixture' (where susceptible and resistant cultivars coexist) to 'pure' strategies (with only resistant cultivar) depending on the resistance characteristics and the epidemiological context (epidemic incidence and landscape connectivity). We demonstrate and discuss gaps concerning virus epidemiology across the agro-ecological interface that must be filled to achieve sustainable disease management. PMID:22260272

  18. Biogas as a source of rural energy

    SciTech Connect

    Kalia, A.K.

    2000-01-01

    The hilly state of Himachal Pradesh, with nearly 2.15 million cattle and 0.7 million buffalo, has the potential to install 0.64 million biogas plants of 1 m{sup 3} size. These plants could generate nearly 4.90 x 105 m{sup 3} of biogas, equivalent to 3.07 x 10{sup 5} L kerosene per day to meet domestic energy needs of nearly one-fourth of its rural population. During 1982--1998, only 12.8% of this potential was achieved. The percent of possible potential achieved in plant installations in 12 districts of this state, namely, Bilaspur, Chamba, Hamirpur, Kangra, Kinnaur, Kullu, Lahul-Spiti, Mandi, Shimla, Sirmour, Solan, and Una, are 35.35, 1.70, 20.96, 8.67, 1.54, 6.96, 0.00, 18.49, 3.84, 8.521, 18.29, and 13.23%, respectively. There is a need to strengthen biogas promotion, particularly in the districts of Kangra, Mandi, Solan, and Una, which range from mid-hill to low-hill terrain and which have large potential due to high concentration of bovine population. Increased costs and comparatively low rate of subsidies has resulted in a decreasing rate of plant installation annually, from 3,500 during 1987--1992 to fewer than 1,200 during 1995--1998. The percentage of functioning plants was 82% in 1987--1988 but has decreased to 63%. To ensure proper installation and functionality of plants, the authors discuss the needed improvements in the biogas promotion program.

  19. Climate change, plant traits, and invasion in natural and agricultural ecosystems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Invasive species and climate change, each of which is likely to influence agricultural productivity and biological diversity, are also likely to interact. This chapter explores characteristics of both invasive plants and invaded ecosystems to search for generalizations that may allow us to predict w...

  20. Agricultural Mechanics Unit for Plant Science Core Curriculum. Volume 15, Number 4. Instructor's Guide.

    ERIC Educational Resources Information Center

    Linhardt, Richard E.; Hunter, Bill

    This instructor's guide is intended for use in teaching the agricultural mechanics unit of a plant science core curriculum. Covered in the individual units of the guide are the following topics: arc welding (following safety procedures, controlling distortion, selecting and caring for electrodes, identifying the material to be welded, and welding…

  1. Pesticide Applicator Certification Training, Manual No. 1a: Agricultural Pest Control. a. Plant.

    ERIC Educational Resources Information Center

    Allen, W. A.; And Others

    This manual provides information needed to meet the minimum standards for certification as an applicator of pesticides in the agricultural plant pest control category. Adapted for the State of Virginia, the text discusses: (1) the basics of insecticides; (2) insect pests; (3) selection and calibration of applicator equipment; and (4) the proper…

  2. Sludge storage lagoon biogas recovery and use. Volume 2

    SciTech Connect

    Muller, D.; Norville, C.

    1991-07-01

    The City of Memphis has two wastewater treatment plants. The SWTP employs two large anaerobic digestion sludge lagoons as part of the overall sludge treatment system. Although these lagoons are effective in concentrating and digesting sludge, they can generate offensive odors. The SWTP uses aerobic digesters to partially stabilize the sludge and help reduce objectionable odors before it enters the lagoons. The anaerobic digestion of sludge in the lagoons results in the dispersion of a large quantity of biogas into the atmosphere. The City realized that if the lagoons could be covered, the odor problem could be resolved, and at the same, time, biogas could be recovered and utilized as a source of energy. In 1987, the City commissioned ADI International to conduct a feasibility study to evaluate alternative methods of covering the lagoons and recovering and utilizing the biogas. The study recommended that the project be developed in two phases: (1) recovery of the biogas and (2) utilization of the biogas. Phase 1 consists of covering the two lagoons with an insulated membrane to control odor and temperature and collect the biogas. Phase 1 was found to be economically feasible and offered a unique opportunity for the City to save substantial operating costs at the treatment facility. The Memphis biogas recovery project is the only application in the world where a membrane cover has been used on a municipal wastewater sludge lagoon. It is also the largest lagoon cover system in the world.

  3. Anaerobic co-digestion of the organic fraction of municipal solid waste with FOG waste from a sewage treatment plant: recovering a wasted methane potential and enhancing the biogas yield.

    PubMed

    Martín-González, L; Colturato, L F; Font, X; Vicent, T

    2010-10-01

    Anaerobic digestion is applied widely to treat the source collected organic fraction of municipal solid wastes (SC-OFMSW). Lipid-rich wastes are a valuable substrate for anaerobic digestion due to their high theoretical methane potential. Nevertheless, although fat, oil and grease waste from sewage treatment plants (STP-FOGW) are commonly disposed of in landfill, European legislation is aimed at encouraging more effective forms of treatment. Co-digestion of the above wastes may enhance valorisation of STP-FOGW and lead to a higher biogas yield throughout the anaerobic digestion process. In the present study, STP-FOGW was evaluated as a co-substrate in wet anaerobic digestion of SC-OFMSW under mesophilic conditions (37 degrees C). Batch experiments carried out at different co-digestion ratios showed an improvement in methane production related to STP-FOGW addition. A 1:7 (VS/VS) STP-FOGW:SC-OFMSW feed ratio was selected for use in performing further lab-scale studies in a 5L continuous reactor. Biogas yield increased from 0.38+/-0.02 L g VS(feed)(-1) to 0.55+/-0.05 L g VS(feed)(-1) as a result of adding STP-FOGW to reactor feed. Both VS reduction values and biogas methane content were maintained and inhibition produced by long chain fatty acid (LCFA) accumulation was not observed. Recovery of a currently wasted methane potential from STP-FOGW was achieved in a co-digestion process with SC-OFMSW. PMID:20400285

  4. Anaerobic co-digestion of the organic fraction of municipal solid waste with FOG waste from a sewage treatment plant: Recovering a wasted methane potential and enhancing the biogas yield

    SciTech Connect

    Martin-Gonzalez, L.; Colturato, L.F.; Font, X.; Vicent, T.

    2010-10-15

    Anaerobic digestion is applied widely to treat the source collected organic fraction of municipal solid wastes (SC-OFMSW). Lipid-rich wastes are a valuable substrate for anaerobic digestion due to their high theoretical methane potential. Nevertheless, although fat, oil and grease waste from sewage treatment plants (STP-FOGW) are commonly disposed of in landfill, European legislation is aimed at encouraging more effective forms of treatment. Co-digestion of the above wastes may enhance valorisation of STP-FOGW and lead to a higher biogas yield throughout the anaerobic digestion process. In the present study, STP-FOGW was evaluated as a co-substrate in wet anaerobic digestion of SC-OFMSW under mesophilic conditions (37 {sup o}C). Batch experiments carried out at different co-digestion ratios showed an improvement in methane production related to STP-FOGW addition. A 1:7 (VS/VS) STP-FOGW:SC-OFMSW feed ratio was selected for use in performing further lab-scale studies in a 5 L continuous reactor. Biogas yield increased from 0.38 {+-} 0.02 L g VS{sub feed}{sup -1} to 0.55 {+-} 0.05 L g VS{sub feed}{sup -1} as a result of adding STP-FOGW to reactor feed. Both VS reduction values and biogas methane content were maintained and inhibition produced by long chain fatty acid (LCFA) accumulation was not observed. Recovery of a currently wasted methane potential from STP-FOGW was achieved in a co-digestion process with SC-OFMSW.

  5. Biogas production: current state and perspectives.

    PubMed

    Weiland, Peter

    2010-01-01

    Anaerobic digestion of energy crops, residues, and wastes is of increasing interest in order to reduce the greenhouse gas emissions and to facilitate a sustainable development of energy supply. Production of biogas provides a versatile carrier of renewable energy, as methane can be used for replacement of fossil fuels in both heat and power generation and as a vehicle fuel. For biogas production, various process types are applied which can be classified in wet and dry fermentation systems. Most often applied are wet digester systems using vertical stirred tank digester with different stirrer types dependent on the origin of the feedstock. Biogas is mainly utilized in engine-based combined heat and power plants, whereas microgas turbines and fuel cells are expensive alternatives which need further development work for reducing the costs and increasing their reliability. Gas upgrading and utilization as renewable vehicle fuel or injection into the natural gas grid is of increasing interest because the gas can be used in a more efficient way. The digestate from anaerobic fermentation is a valuable fertilizer due to the increased availability of nitrogen and the better short-term fertilization effect. Anaerobic treatment minimizes the survival of pathogens which is important for using the digested residue as fertilizer. This paper reviews the current state and perspectives of biogas production, including the biochemical parameters and feedstocks which influence the efficiency and reliability of the microbial conversion and gas yield. PMID:19777226

  6. Bioenergy systems report. April 1984: innovations in biogas systems and technology

    SciTech Connect

    Mahin, D.B.

    1984-04-01

    A comprehensive review of recent innovations in the design and operation of biogas systems is presented. The report contains information on about sixty distinct designs for biogas plants. These include plants designed for use by individual families, larger farm plants, plants used for the digestion of agroindustrial residues, and plants producing or recovering biogas from human wastes and residues. Some have been designed for use in developing countries; others have been used primarily in developed countries but may involve design elements that could be utilized in developing countries.

  7. Increased biogas production in a wastewater treatment plant by anaerobic co-digestion of fruit and vegetable waste and sewer sludge - a full scale study.

    PubMed

    Park, Nathan D; Thring, Ronald W; Garton, Randy P; Rutherford, Michael P; Helle, Steve S

    2011-01-01

    Anaerobic digestion is a well established technology for the reduction of organic matter and stabilization of wastewater. Biogas, a mixture of methane and carbon dioxide, is produced as a useful by-product of the process. Current solid waste management at the city of Prince George is focused on disposal of waste and not on energy recovery. Co-digestion of fresh fruit and vegetable waste with sewer sludge can improve biogas yield by increasing the load of biodegradable material. A six week full-scale project co-digesting almost 15,000 kg of supermarket waste was completed. Average daily biogas production was found to be significantly higher than in previous years. Digester operation remained stable over the course of the study as indicated by the consistently low volatile acids-to-alkalinity ratio. Undigested organic material was visible in centrifuged sludge suggesting that the waste should have been added to the primary digester to prevent short circuiting and to increase the hydraulic retention time of the freshly added waste. PMID:22020478

  8. Role of Plant Growth Promoting Rhizobacteria in Agricultural Sustainability-A Review.

    PubMed

    Vejan, Pravin; Abdullah, Rosazlin; Khadiran, Tumirah; Ismail, Salmah; Nasrulhaq Boyce, Amru

    2016-01-01

    Plant growth promoting rhizobacteria (PGPR) shows an important role in the sustainable agriculture industry. The increasing demand for crop production with a significant reduction of synthetic chemical fertilizers and pesticides use is a big challenge nowadays. The use of PGPR has been proven to be an environmentally sound way of increasing crop yields by facilitating plant growth through either a direct or indirect mechanism. The mechanisms of PGPR include regulating hormonal and nutritional balance, inducing resistance against plant pathogens, and solubilizing nutrients for easy uptake by plants. In addition, PGPR show synergistic and antagonistic interactions with microorganisms within the rhizosphere and beyond in bulk soil, which indirectly boosts plant growth rate. There are many bacteria species that act as PGPR, described in the literature as successful for improving plant growth. However, there is a gap between the mode of action (mechanism) of the PGPR for plant growth and the role of the PGPR as biofertilizer-thus the importance of nano-encapsulation technology in improving the efficacy of PGPR. Hence, this review bridges the gap mentioned and summarizes the mechanism of PGPR as a biofertilizer for agricultural sustainability. PMID:27136521

  9. Pivoting from Arabidopsis to wheat to understand how agricultural plants integrate responses to biotic stress.

    PubMed

    Harris, M O; Friesen, T L; Xu, S S; Chen, M S; Giron, D; Stuart, J J

    2015-02-01

    In this review, we argue for a research initiative on wheat's responses to biotic stress. One goal is to begin a conversation between the disparate communities of plant pathology and entomology. Another is to understand how responses to a variety of agents of biotic stress are integrated in an important crop. We propose gene-for-gene interactions as the focus of the research initiative. On the parasite's side is an Avirulence (Avr) gene that encodes one of the many effector proteins the parasite applies to the plant to assist with colonization. On the plant's side is a Resistance (R) gene that mediates a surveillance system that detects the Avr protein directly or indirectly and triggers effector-triggered plant immunity. Even though arthropods are responsible for a significant proportion of plant biotic stress, they have not been integrated into important models of plant immunity that come from plant pathology. A roadblock has been the absence of molecular evidence for arthropod Avr effectors. Thirty years after this evidence was discovered in a plant pathogen, there is now evidence for arthropods with the cloning of the Hessian fly's vH13 Avr gene. After reviewing the two models of plant immunity, we discuss how arthropods could be incorporated. We end by showing features that make wheat an interesting system for plant immunity, including 479 resistance genes known from agriculture that target viruses, bacteria, fungi, nematodes, insects, and mites. It is not likely that humans will be subsisting on Arabidopsis in the year 2050. It is time to start understanding how agricultural plants integrate responses to biotic stress. PMID:25504642

  10. Biogas as a fuel source for SOFC co-generators

    NASA Astrophysics Data System (ADS)

    Van herle, Jan; Membrez, Yves; Bucheli, Olivier

    This study reports on the combination of solid oxide fuel cell (SOFC) generators fueled with biogas as renewable energy source, recoverable from wastes but at present underexploited. From a mobilisable near-future potential in the European Union (EU-15) of 17 million tonnes oil equivalent (Mtoe), under 15% appears to be converted today into useful heat and power (2 Mtoe). SOFCs could improve and promote the exploitation of biogas on manifold generation sites as small combined heat and power (5-50 kW el), especially for farm and sewage installations, raising the electrical conversion efficiency on such reduced and variable power level. Larger module packs of the high temperature ceramic converter would also be capable of operating on contaminated fuel of low heating value (less than 40% that of natural gas) which can emanate from landfill sites (MW-size). Landfill gas delivers 80% of current world biogas production. This document compiles and estimates biogas data on actual production and future potential and presents the thermodynamics of the biogas reforming and electrochemical conversion processes. A case study is reported of the energy balance of a small SOFC co-generator operated with agricultural biogas, the largest potential source.

  11. Microalgae as substrates for fermentative biogas production in a combined biorefinery concept.

    PubMed

    Mussgnug, J H; Klassen, V; Schlüter, A; Kruse, O

    2010-10-01

    Most organic matter can be used for bioenergy generation via anaerobic fermentation. Today, crop plants like maize play the dominant role as substrates for renewable biogas production. In this work we investigated the suitability of six dominant microalgae species (freshwater and saltwater algae and cyanobacteria) as alternative substrates for biogas production. We could demonstrate that the biogas potential is strongly dependent on the species and on the pretreatment. Fermentation of the green alga Chlamydomonas reinhardtii was efficient with a production of 587 ml(±8.8 SE) biogas g volatile solids(-1) (VS(-1)), whereas fermentation of Scenedesmus obliquus was inefficient with only 287 ml(±10.1 SE) biogas g VS(-1) being produced. Drying as a pretreatment decreased the amount of biogas production to ca. 80%. The methane content of biogas from microalgae was 7-13% higher compared to biogas from maize silage. To evaluate integrative biorefinery concepts, hydrogen production in C. reinhardtii prior to anaerobic fermentation of the algae biomass was measured and resulted in an increase of biogas generation to 123% (±3.7 SE). We conclude that selected algae species can be good substrates for biogas production and that anaerobic fermentation can seriously be considered as final step in future microalgae-based biorefinery concepts. PMID:20691224

  12. Influence of copper on root exudate patterns in some metallophytes and agricultural plants.

    PubMed

    Meier, S; Alvear, M; Borie, F; Aguilera, P; Ginocchio, R; Cornejo, P

    2012-01-01

    A hydroponic experiment was carried out to determine the root exudation patterns in two Cu-metallophytes (Oenothera picensis and Imperata condensata) and two agricultural plants (Lupinus albus and Helianthus annuus). Plants were grown in nutrient solution at increasing Cu doses (0, 0.125, 0.25, 0.5, 1 and 2mgCuL(-1)), and plant growth, root elongation, Cu accumulation and root exudates were measured. All plants showed a decrease of over 60% in root elongation at the highest Cu supply level, being O. picensis the most sensitive specie and showing the highest shoot and root Cu concentrations (116 and 2657μgCug(-1), respectively), which were six fold higher than the other species. Differences in root exudation patterns of low molecular weight organic acids were found, with extremely high amounts of succinic acid exuded by O. picensis (1049μmolg(-1)h(-1)), and citric acid by I. condensata (164μmolg(-1)h(-1)). In metallophytes, the organic acid exudation was increased even with no root elongation, meanwhile agricultural plants exuded citric acid at constant levels. Exudation of phenolic compounds was highly species-dependent, with catechin mainly exuded by I. condensata, (2.62μmolg(-1)h(-1)) cinnamic acid by O. picensis (5.08μmolg(-1)h(-1)) and coumaric acid exclusively exuded by H. annuus (13.6μmolg(-1)h(-1)) at high Cu levels. These results indicated that differences in root exudation patterns among metallophytes and agricultural plants could affect their Cu tolerance. Particularly, the higher exudation rate showed by I. condensata can be an effective exclusion mechanism to tolerate high Cu concentrations, supporting its use in Cu phytostabilization programs. PMID:21937112

  13. Fabrication Of Biogenic Silver Nanoparticles Using Agricultural Crop Plant Leaf Extracts

    NASA Astrophysics Data System (ADS)

    Rajani, P.; SriSindhura, K.; Prasad, T. N. V. K. V.; Hussain, O. M.; Sudhakar, P.; Latha, P.; Balakrishna, M.; Kambala, V.; Reddy, K. Raja

    2010-10-01

    Nanoparticles are being viewed as fundamental building blocks of nanotechnology. Biosynthesis of nanoparticles by plant extracts is currently under exploitation. Use of agricultural crop plant extracts for synthesis of metal nanoparticles would add a new dimension to the agricultural sector in the utilization of crop waste. Silver has long been recognized as having an inhibitory effect towards many bacterial strains and microorganisms commonly present in medical and industrial processes. Four pulse crop plants and three cereal crop plants (Vigna radiata, Arachis hypogaea, Cyamopsis tetragonolobus, Zea mays, Pennisetum glaucum, Sorghum vulgare) were used and compared for their extra cellular synthesis of metallic silver nanoparticles. Stable silver nanoparticles were formed by treating aqueous solution of AgNO3 with the plant leaf extracts as reducing agent at temperatures 50 °C-95 °C. UV-Visible spectroscopy was utilized to monitor the formation of silver nanoparticles. XRD analysis of formed silver nanoparticles revealed face centered cubic structure with (111), (200), (220) and (311) planes. SEM and EDAX analysis confirm the size of the formed silver nanoparticles to be in the range of 50-200 nm. Our proposed work offers a enviro-friendly method for biogenic silver nanoparticles production. This could provide a faster synthesis rate comparable to those of chemical methods and potentially be used in areas such as cosmetics, food and medical applications.

  14. Silicon: Potential to Promote Direct and Indirect Effects on Plant Defense Against Arthropod Pests in Agriculture

    PubMed Central

    Reynolds, Olivia L.; Padula, Matthew P.; Zeng, Rensen; Gurr, Geoff M.

    2016-01-01

    Silicon has generally not been considered essential for plant growth, although it is well recognized that many plants, particularly Poaceae, have substantial plant tissue concentrations of this element. Recently, however, the International Plant Nutrition Institute [IPNI] (2015), Georgia, USA has listed it as a “beneficial substance”. This reflects that numerous studies have now established that silicon may alleviate both biotic and abiotic stress. This paper explores the existing knowledge and recent advances in elucidating the role of silicon in plant defense against biotic stress, particularly against arthropod pests in agriculture and attraction of beneficial insects. Silicon confers resistance to herbivores via two described mechanisms: physical and biochemical/molecular. Until recently, studies have mainly centered on two trophic levels; the herbivore and plant. However, several studies now describe tri-trophic effects involving silicon that operate by attracting predators or parasitoids to plants under herbivore attack. Indeed, it has been demonstrated that silicon-treated, arthropod-attacked plants display increased attractiveness to natural enemies, an effect that was reflected in elevated biological control in the field. The reported relationships between soluble silicon and the jasmonic acid (JA) defense pathway, and JA and herbivore-induced plant volatiles (HIPVs) suggest that soluble silicon may enhance the production of HIPVs. Further, it is feasible that silicon uptake may affect protein expression (or modify proteins structurally) so that they can produce additional, or modify, the HIPV profile of plants. Ultimately, understanding silicon under plant ecological, physiological, biochemical, and molecular contexts will assist in fully elucidating the mechanisms behind silicon and plant response to biotic stress at both the bi- and tri-trophic levels. PMID:27379104

  15. Silicon: Potential to Promote Direct and Indirect Effects on Plant Defense Against Arthropod Pests in Agriculture.

    PubMed

    Reynolds, Olivia L; Padula, Matthew P; Zeng, Rensen; Gurr, Geoff M

    2016-01-01

    Silicon has generally not been considered essential for plant growth, although it is well recognized that many plants, particularly Poaceae, have substantial plant tissue concentrations of this element. Recently, however, the International Plant Nutrition Institute [IPNI] (2015), Georgia, USA has listed it as a "beneficial substance". This reflects that numerous studies have now established that silicon may alleviate both biotic and abiotic stress. This paper explores the existing knowledge and recent advances in elucidating the role of silicon in plant defense against biotic stress, particularly against arthropod pests in agriculture and attraction of beneficial insects. Silicon confers resistance to herbivores via two described mechanisms: physical and biochemical/molecular. Until recently, studies have mainly centered on two trophic levels; the herbivore and plant. However, several studies now describe tri-trophic effects involving silicon that operate by attracting predators or parasitoids to plants under herbivore attack. Indeed, it has been demonstrated that silicon-treated, arthropod-attacked plants display increased attractiveness to natural enemies, an effect that was reflected in elevated biological control in the field. The reported relationships between soluble silicon and the jasmonic acid (JA) defense pathway, and JA and herbivore-induced plant volatiles (HIPVs) suggest that soluble silicon may enhance the production of HIPVs. Further, it is feasible that silicon uptake may affect protein expression (or modify proteins structurally) so that they can produce additional, or modify, the HIPV profile of plants. Ultimately, understanding silicon under plant ecological, physiological, biochemical, and molecular contexts will assist in fully elucidating the mechanisms behind silicon and plant response to biotic stress at both the bi- and tri-trophic levels. PMID:27379104

  16. Not all GMOs are crop plants: non-plant GMO applications in agriculture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In the time since the tools of modern biotechnology have become available, the most commonly applied and often discussed genetically modified organisms are genetically modified crop plants, although genetic engineering is also being used successfully in organisms other than plants, including bacteri...

  17. Planting the seed: the 2009 Midwest Rural Agricultural Safety and Health Forum (MRASH).

    PubMed

    Donham, Kelley J

    2010-04-01

    The annual Midwest Rural Agricultural Safety and Health Forum (MRASH) is produced by the combined efforts and resources of Iowa's Center for Agricultural Safety and Health (I-CASH), the Great Plains Center for Agricultural Health, and The Heartland Center for Occupational Health. There is a regional focus for the conference on agricultural health issues for the 11 states of the upper Midwest, thus the new name for conference (MRASH). The purpose of this conference is to "plant the seeds" for stimulating and strengthening collaborative efforts among researchers, practitioner, agricultural producers, and medical professionals. This is accomplished by sharing cutting edge research, reviewing intervention theory and methodologies, reviewing activities of regional academic, public health, and nonprofit farm health and safety organization. The 2009 conference included plenary and topical breakout sessions. The plenary sessions included topics that have a relevance to the theory and practice of health interventions in populations. The breakout sessions were quite varied, with topics of community prevention programs (including Certified Safe Farm for large farms), AgrAbility programs, exposures from noise, pesticides, needle sticks, and all-terrain vehicle operation. The largest breakout session was on methicillin-resistant Staphylococcus aureus (MRSA). Should there be further questions of authors or the organizing committee, contact information is available at the following Web site address: http://www.public-health.uiowa.edu/ICASH/programs/MRASH/2009/index.htm. PMID:20407986

  18. Functional roles of melatonin in plants, and perspectives in nutritional and agricultural science.

    PubMed

    Tan, Dun-Xian; Hardeland, Rudiger; Manchester, Lucien C; Korkmaz, Ahmet; Ma, Shuran; Rosales-Corral, Sergio; Reiter, Russel J

    2012-01-01

    The presence of melatonin in plants is universal. Evidence has confirmed that a major portion of the melatonin is synthesized by plants themselves even though a homologue of the classic arylalkylamine N-acetyltransferase (AANAT) has not been identified as yet in plants. Thus, the serotonin N-acetylating enzyme in plants may differ greatly from the animal AANAT with regard to sequence and structure. This would imply multiple evolutionary origins of enzymes with these catalytic properties. A primary function of melatonin in plants is to serve as the first line of defence against internal and environmental oxidative stressors. The much higher melatonin levels in plants compared with those found in animals are thought to be a compensatory response by plants which lack means of mobility, unlike animals, as a means of coping with harsh environments. Importantly, remarkably high melatonin concentrations have been measured in popular beverages (coffee, tea, wine, and beer) and crops (corn, rice, wheat, barley, and oats). Billions of people worldwide consume these products daily. The beneficial effects of melatonin on human health derived from the consumption of these products must be considered. Evidence also indicates that melatonin has an ability to increase the production of crops. The mechanisms may involve the roles of melatonin in preservation of chlorophyll, promotion of photosynthesis, and stimulation of root development. Transgenic plants with enhanced melatonin content could probably lead to breakthroughs to increase crop production in agriculture and to improve the general health of humans. PMID:22016420

  19. Addressing case specific biogas plant tasks: industry oriented methane yields derived from 5L Automatic Methane Potential Test Systems in batch or semi-continuous tests using realistic inocula, substrate particle sizes and organic loading.

    PubMed

    Kolbl, Sabina; Paloczi, Attila; Panjan, Jože; Stres, Blaž

    2014-02-01

    The primary aim of the study was to develop and validate an in-house upscale of Automatic Methane Potential Test System II for studying real-time inocula and real-scale substrates in batch, codigestion and enzyme enhanced hydrolysis experiments, in addition to semi-continuous operation of the developed equipment and experiments testing inoculum functional quality. The successful upscale to 5L enabled comparison of different process configurations in shorter preparation times with acceptable accuracy and high-through put intended for industrial decision making. The adoption of the same scales, equipment and methodologies in batch and semi-continuous tests mirroring those at full scale biogas plants resulted in matching methane yields between the two laboratory tests and full-scale, confirming thus the increased decision making value of the approach for industrial operations. PMID:24368269

  20. Emergy analysis of biogas systems based on different raw materials.

    PubMed

    Wang, Yang; Lin, Cong; Li, Jing; Duan, Na; Li, Xue; Fu, Yanyan

    2013-01-01

    Environmental pollution and energy crisis restrict the development of China, and the utilization of renewable technology is an effective strategy to alleviate the damage. Biogas engineering has rapidly developed attributes to solve environmental problems and create a renewable energy product biogas. In this paper, two different biogas plants' materials were analyzed by emergy method. One of them is a biogas project whose degraded material is feces (BPF system), and the other is the one whose degraded material is corn straw (BPC system). As a result, the ecological-economic values of BPF and BPC are $28,300/yr and $8,100/yr, respectively. Considering currency, environment, and human inputs, both of the biogas projects have the ability of disposing waste and potential for development. The proportion of biogas output is much more than fertilizer output; so, fertilizer utilization should be emphasized in the future. In comparison, BPF is better than BPC in the aspects of ecological-economic benefits, environmental benefits, and sustainability. The reason is the difficulty of corn straw seasonal collection and degradation. Thus it is proposed that BPC should be combined with the other raw materials. PMID:23476134

  1. Emergy Analysis of Biogas Systems Based on Different Raw Materials

    PubMed Central

    Wang, Yang; Lin, Cong; Li, Jing; Duan, Na; Li, Xue; Fu, Yanyan

    2013-01-01

    Environmental pollution and energy crisis restrict the development of China, and the utilization of renewable technology is an effective strategy to alleviate the damage. Biogas engineering has rapidly developed attributes to solve environmental problems and create a renewable energy product biogas. In this paper, two different biogas plants' materials were analyzed by emergy method. One of them is a biogas project whose degraded material is feces (BPF system), and the other is the one whose degraded material is corn straw (BPC system). As a result, the ecological-economic values of BPF and BPC are $28,300/yr and $8,100/yr, respectively. Considering currency, environment, and human inputs, both of the biogas projects have the ability of disposing waste and potential for development. The proportion of biogas output is much more than fertilizer output; so, fertilizer utilization should be emphasized in the future. In comparison, BPF is better than BPC in the aspects of ecological-economic benefits, environmental benefits, and sustainability. The reason is the difficulty of corn straw seasonal collection and degradation. Thus it is proposed that BPC should be combined with the other raw materials. PMID:23476134

  2. Linking agricultural practices, mycorrhizal fungi, and traits mediating plant-insect interactions.

    PubMed

    Barber, Nicholas A; Kiers, E Toby; Theis, Nina; Hazzard, Ruth V; Adler, Lynn S

    2013-10-01

    Agricultural management has profound effects on soil communities. Activities such as fertilizer inputs can modify the composition of arbuscular mycorrhizal fungi (AMF) communities, which form important symbioses with the roots of most crop plants. Intensive conventional agricultural management may select for less mutualistic AMF with reduced benefits to host plants compared to organic management, but these differences are poorly understood. AMF are generally evaluated based on their direct growth effects on plants. However, mycorrhizal colonization also may alter plant traits such as tissue nutrients, defensive chemistry, or floral traits, which mediate important plant-insect interactions like herbivory and pollination. To determine the effect of AMF from different farming practices on plant performance and traits that putatively mediate species interactions, we performed a greenhouse study by inoculating Cucumis sativus (cucumber, Cucurbitaceae) with AMF from conventional farms, organic farms, and a commercial AMF inoculum. We measured growth and a suite of plant traits hypothesized to be important predictors of herbivore resistance and pollinator attraction. Several leaf and root traits and flower production were significantly affected by AMF inoculum. Both conventional and organic AMF reduced leaf P content but increased Na content compared to control and commercial AMF. Leaf defenses were unaffected by AMF treatments, but conventional AMF increased root cucurbitacin C, the primary defensive chemical of C. sativus, compared to organic AMF. These effects may have important consequences for herbivore preference and population dynamics. AMF from both organic and conventional farms decreased flower production relative to commercial and control treatments, which may reduce pollinator attraction and plant reproduction. AMF from both farm types also reduced seed germination, but effects on plant growth were limited. Our results suggest that studies only considering AMF

  3. Environmental effects of planting biomass crops at larger scales on agricultural lands

    SciTech Connect

    Tolbert, V.R.; Downing, M.E.

    1995-09-01

    Increasing from research-scale to larger-scale plantings of herbaceous. and short rotation woody crops on agricultural land in the United States has raised questions about the positive and negative environmental effects of farmland conversion. Research currently underway at experimental plot scales enables us examine runoff quality and quantity, erosion, and changes in soil characteristics associated with these energy crops compared to conventional row crops. A study of the fate of chemicals applied to the different crop types will enhance our knowledge of uptake, release, and off-site movement of nutrients and pesticides. Ongoing biodiversity studies in the North Central US allow us to compare differences in scale of plantings on bird and small mammal populations and habitat use. Plantings of 50--100 or more contiguous acres are needed to allow both researchers and producers to determine the benefits of including temporal energy crop rotations in the landscape. Results from these larger-scale plantings will help identify (1) the monitoring requirements needed to determine environmental effects of larger-scale plantings, (2) the best methods to determine the environmental effects of rotation length and the best crop management strategies for full-scale production. Because of the variations in soils, temperature, rainfall and other climatic conditions, as well as differences in the types of energy crops most suited for different regions, monitoring of large-scale plantings in these different regions of the US will be required to predict the environmental effects of regional agricultural land-use shifts for full-scale plantings.

  4. Environmental effects of planting energy crops at larger scales on agricultural lands

    SciTech Connect

    Tolbert, V.R.; Downing, M.

    1995-09-01

    Increasing from research-scale to larger-scale plantings of herbaceous and short rotation woody crops on agricultural land in the United States has raised questions about the positive and negative environmental effects of farmland conversion. Research currently underway at experimental plot scales enables us examine runoff quality and quantity, erosion, and changes in soil characteristics associated with these energy crops compared to conventional row crops. A study of the fate of chemicals applied to the different crop types will enhance our knowledge of uptake, release, and off-site movement of nutrients and pesticides. Ongoing biodiversity studies in the North Central US allow us to compare differences in scale of plantings on bird and small mammal populations and habitat use. Plantings of 50--100 or more contiguous acres are needed to allow both researchers and producers to determine the benefits of including temporal energy crop rotations in the landscape. Results from these larger-scale plantings will help identify (1) the monitoring requirements needed to determine environmental effects of larger-scale plantings, (2) the best methods to determine the environmental effects of rotation length and the best crop management strategies for full-scale production. Because of the variations in soils, temperature, rainfall and other climatic conditions, as well as differences in the types of energy crops most suited for different regions, monitoring of large-scale plantings in these different regions of the US will be required to predict the environmental effects of regional agricultural land-use shifts for full-scale plantings.

  5. Environmental and economic analysis of application of water hyacinth for eutrophic water treatment coupled with biogas production.

    PubMed

    Wang, Zanxin; Calderon, Margaret M

    2012-11-15

    The proliferation of water hyacinth is currently controlled by removing it from a water body and disposing it by landfill in China. Using water hyacinth to remove nutrients from water bodies and to produce biogas is another technically feasible option for the control of water hyacinth, but its environmental and economic performances are not well understood. This study collected data from an experimental biogas plant to develop a lifecycle analysis and a cost benefit analysis for the control of water hyacinth proliferation in a eutrophic lake in China. Comparison was made between the alternative option of using water hyacinth for biogas production and the current practice of disposing it in landfills. The results reveal that the biogas option is economically feasible with a positive energy balance. The removal of water hyacinth to produce biogas can contribute to water quality improvement and GHG emission reduction whose values, however, depend on the processing scale of the biogas plant. Since both the current approach and the biogas option can remove nutrients from water bodies, the additional value of water quality improvement resulting from the biogas option is only possible when the processing scale of the biogas plant is greater than the amount of water hyacinth disposed by landfill. The emission of methane deserves attention when water hyacinth is disposed by landfill. The biogas option can respond to China's policies on water pollution control, renewable energy development, and energy saving and emission reduction. PMID:22813757

  6. Phytoremediation potential of some agricultural plants on heavy metal contaminated mine waste soils, salem district, tamilnadu.

    PubMed

    Padmapriya, S; Murugan, N; Ragavendran, C; Thangabalu, R; Natarajan, D

    2016-01-01

    The Pot culture experiment performed for phytoextraction potential of selected agricultural plants [millet (Eleusine coracana), mustard (Brassica juncea), jowar (Sorghum bicolor), black gram (Vigna mungo), pumpkin (Telfairia occidentalis)] grown in metal contaminated soils around the Salem region, Tamilnadu, India. Physiochemical characterization of soils, reported as low to medium level of N, P, K was found in test soils. The Cr content higher in mine soils than control and the values are 0.176 mg/L in Dalmia soil and 0.049 mg/L in Burn & Co soil. The germination rate low in mine soil than control soils (25 to 85%). The content of chlorophyll, carotenoid, carbohydrate and protein decreased in mine soils than control. The morphological parameters and biomass values decreased in experimental plants due to metal accumulation. Proline content increased in test plants and ranged from 0.113 mg g(-1) to 0.858 mg g(-1) which indicate the stress condition due to toxicity of metals. Sorghum and black gram plants reported as metal tolerant capacity. Among the plants, Sorghum produced good results (both biomass and biochemical parameters) which equal to control plant and suggests Sorghum plant is an ideal for remediation of metal contaminated soils. PMID:26366709

  7. Technological assumptions for biogas purification.

    PubMed

    Makareviciene, Violeta; Sendzikiene, Egle

    2015-01-01

    Biogas can be used in the engines of transport vehicles and blended into natural gas networks, but it also requires the removal of carbon dioxide, hydrogen sulphide, and moisture. Biogas purification process flow diagrams have been developed for a process enabling the use of a dolomite suspension, as well as for solutions obtained by the filtration of the suspension, to obtain biogas free of hydrogen sulphide and with a carbon dioxide content that does not exceed 2%. The cost of biogas purification was evaluated on the basis of data on biogas production capacity and biogas production cost obtained from local water treatment facilities. It has been found that, with the use of dolomite suspension, the cost of biogas purification is approximately six times lower than that in the case of using a chemical sorbent such as monoethanolamine. The results showed travelling costs using biogas purified by dolomite suspension are nearly 1.5 time lower than travelling costs using gasoline and slightly lower than travelling costs using mineral diesel fuel. PMID:25609385

  8. Nitrogen uptake, assimilation and remobilization in plants: challenges for sustainable and productive agriculture

    PubMed Central

    Masclaux-Daubresse, Céline; Daniel-Vedele, Françoise; Dechorgnat, Julie; Chardon, Fabien; Gaufichon, Laure; Suzuki, Akira

    2010-01-01

    Background Productive agriculture needs a large amount of expensive nitrogenous fertilizers. Improving nitrogen use efficiency (NUE) of crop plants is thus of key importance. NUE definitions differ depending on whether plants are cultivated to produce biomass or grain yields. However, for most plant species, NUE mainly depends on how plants extract inorganic nitrogen from the soil, assimilate nitrate and ammonium, and recycle organic nitrogen. Efforts have been made to study the genetic basis as well as the biochemical and enzymatic mechanisms involved in nitrogen uptake, assimilation, and remobilization in crops and model plants. The detection of the limiting factors that could be manipulated to increase NUE is the major goal of such research. Scope An overall examination of the physiological, metabolic, and genetic aspects of nitrogen uptake, assimilation and remobilization is presented in this review. The enzymes and regulatory processes manipulated to improve NUE components are presented. Results obtained from natural variation and quantitative trait loci studies are also discussed. Conclusions This review presents the complexity of NUE and supports the idea that the integration of the numerous data coming from transcriptome studies, functional genomics, quantitative genetics, ecophysiology and soil science into explanatory models of whole-plant behaviour will be promising. PMID:20299346

  9. Agricultural recycling of treatment-plant sludge: a case study for a vegetable-processing factory.

    PubMed

    Dolgen, Deniz; Alpaslan, M Necdet; Delen, Nafiz

    2007-08-01

    The present study evaluated the possibility of using the sludge produced by a vegetable-processing factory in agriculture. The sludge was amended with a soil mixture (i.e., a mixture of sand, soil, and manure) and was applied at 0, 165, 330, 495 and 660 t/ha to promote the growth of cucumbers. The effects of various sludge loadings on plant growth were assessed by counting plants and leaves, measuring stem lengths, and weighing the green parts and roots of the plants. We also compared heavy metal uptake by the plants for sludge loadings of 330, 495, and 660 t/ha with various recommended standards for vegetables. Our results showed that plant growth patterns were influenced to some extent by the sludge loadings. In general, the number of leaves, stem length, and dry weight of green parts exhibited a pronounced positive growth response compared with an unfertilized control, and root growth showed a lesser but still significant response at sludge loadings of 165 and 330 t/ha. The sludge application caused no significant increase in heavy metal concentrations in the leaves, though zinc (Zn) and iron (Fe) were found at elevated concentrations. However, despite the Zn and Fe accumulation, we observed no toxicity symptoms in the plants. This may be a result of cucumber's tolerance of high metal levels. PMID:16934389

  10. Belowground Water Dynamics Under Contrasting Annual and Perennial Plant Communities in an Agriculturally-Dominated Landscape

    NASA Astrophysics Data System (ADS)

    Mora, G.; Asbjornsen, H.; Helmers, M. J.; Shepherd, G. W.

    2005-12-01

    The conversion from grasslands and forests to row-crops in the Midwest has affected soil water cycling because plant characteristics are one of the main parameters determining soil storage capacity, infiltration rates, and surface runoff. Little is known, however, about the extent of modification of soil water dynamics under different plant communities. To address this important issue, we are documenting soil water dynamics under contrasting perennial and annual plant communities in an agriculturally-dominated landscape. Measurements of soil moisture and depths of uptake of source water were obtained for six vegetative cover types (corn and soybean field, brome pasture, degraded savanna, restored savanna, and restored prairie) at the Neal Smith National Wildlife Refuge in Prairie City, Iowa. The depths of uptake of soil water were determined on the basis of oxygen isotope composition of soil water and stem water. Measurements were performed once a month during an entire growing season. Preliminary results indicate that soil water present under the different vegetation types show similar profiles with depth during the dry months. Soil water in the upper 5 cm is enriched in oxygen-18 by about 5 per mil relative to soil water at 100 cm. Our preliminary results also indicate that the isotopic composition of stem water from annual plants is typically higher by about 2 per mil relative to that of stem water from perennial plants during the dry period. Whereas the oxygen isotopic composition for corn stem water is -5.49 per mil, that for elm and oak stem water is -7.62 and -7.51 per mil, respectively. The higher isotope values for corn suggest that annual crop plants are withdrawing water from shallower soil horizons relative to perennial plants. Moreover, our preliminary data suggest lower moisture content in soil under annual plant cover. We propose that the presence of deeper roots in the perennial vegetation allows these plants to tap into deeper water sources when

  11. Initial Effects of Differently Treated Biogas Residues from Municipal and Industrial Wastes on Spring Barley Yield Formation.

    PubMed

    Prays, Nadia; Kaupenjohann, Martin

    2016-01-01

    Soil application of biogas residues (BGRs) is important for closing nutrient cycles. This study examined the efficiency and impact on yields and yield formation of solid-liquid separated residues from biodegradable municipal and industrial wastes (bio-waste) in comparison to complete BGRs, nitrification inhibitor, agricultural BGRs, mineral fertilizer and unfertilized plots as control. The experiment was set up as a randomized block design on silt loam Cambisol. Biogas residues from four biogas plants were evaluated. Plants per m², ears per plant, grains per ear and thousand grain weight (TGW) were measured at harvest. Fertilization with BGRs resulted in similar biomass yields compared with mineral fertilizer. Mineral fertilizer (71 dt/ha) and plots fertilized with liquid fraction (59-62 dt/ha) indicated a trend to higher yields than solid fraction or complete BGR due to its high ammonia content. Liquid fractions and fraction with nitrification inhibitor induced fewer plants per m² than corresponding solid and complete variants due to a potential phytotoxicity of high NH4-N concentration during germination. However, barley on plots fertilized with liquid fraction compensated the disadvantages at the beginning during the vegetation period and induced higher grain yields than solid fraction. This was attributable to a higher number of ears per plant and grains per ear. In conclusion, BGRs from biodegradable municipal and industrial wastes can be used for soil fertilization and replace considerable amounts of mineral fertilizer. Our study showed that direct application of the liquid fraction of BGR is the most suitable strategy to achieve highest grain yields. Nevertheless potential phytotoxicity of the high NH4-N concentration in the liquid fraction should be considered. PMID:27116355

  12. Initial Effects of Differently Treated Biogas Residues from Municipal and Industrial Wastes on Spring Barley Yield Formation

    PubMed Central

    Prays, Nadia; Kaupenjohann, Martin

    2016-01-01

    Soil application of biogas residues (BGRs) is important for closing nutrient cycles. This study examined the efficiency and impact on yields and yield formation of solid-liquid separated residues from biodegradable municipal and industrial wastes (bio-waste) in comparison to complete BGRs, nitrification inhibitor, agricultural BGRs, mineral fertilizer and unfertilized plots as control. The experiment was set up as a randomized block design on silt loam Cambisol. Biogas residues from four biogas plants were evaluated. Plants per m², ears per plant, grains per ear and thousand grain weight (TGW) were measured at harvest. Fertilization with BGRs resulted in similar biomass yields compared with mineral fertilizer. Mineral fertilizer (71 dt/ha) and plots fertilized with liquid fraction (59–62 dt/ha) indicated a trend to higher yields than solid fraction or complete BGR due to its high ammonia content. Liquid fractions and fraction with nitrification inhibitor induced fewer plants per m² than corresponding solid and complete variants due to a potential phytotoxicity of high NH4-N concentration during germination. However, barley on plots fertilized with liquid fraction compensated the disadvantages at the beginning during the vegetation period and induced higher grain yields than solid fraction. This was attributable to a higher number of ears per plant and grains per ear. In conclusion, BGRs from biodegradable municipal and industrial wastes can be used for soil fertilization and replace considerable amounts of mineral fertilizer. Our study showed that direct application of the liquid fraction of BGR is the most suitable strategy to achieve highest grain yields. Nevertheless potential phytotoxicity of the high NH4-N concentration in the liquid fraction should be considered. PMID:27116355

  13. An intellectual property sharing initiative in agricultural biotechnology: development of broadly accessible technologies for plant transformation.

    PubMed

    Chi-Ham, Cecilia L; Boettiger, Sara; Figueroa-Balderas, Rosa; Bird, Sara; Geoola, Josef N; Zamora, Pablo; Alandete-Saez, Monica; Bennett, Alan B

    2012-06-01

    The Public Intellectual Property Resource for Agriculture (PIPRA) was founded in 2004 by the Rockefeller Foundation in response to concerns that public investments in agricultural biotechnology benefiting developing countries were facing delays, high transaction costs and lack of access to important technologies due to intellectual property right (IPR) issues. From its inception, PIPRA has worked broadly to support a wide range of research in the public sector, in specialty and minor acreage crops as well as crops important to food security in developing countries. In this paper, we review PIPRA's work, discussing the failures, successes, and lessons learned during its years of operation. To address public sector's limited freedom-to-operate, or legal access to third-party rights, in the area of plant transformation, we describe PIPRA's patent 'pool' approach to develop open-access technologies for plant transformation which consolidate patent and tangible property rights in marker-free vector systems. The plant transformation system has been licensed and deployed for both commercial and humanitarian applications in the United States (US) and Africa, respectively. PMID:22221977

  14. The plant breeding industry after pure line theory: Lessons from the National Institute of Agricultural Botany.

    PubMed

    Berry, Dominic

    2014-06-01

    In the early twentieth century, Wilhelm Johannsen proposed his pure line theory and the genotype/phenotype distinction, work that is prized as one of the most important founding contributions to genetics and Mendelian plant breeding. Most historians have already concluded that pure line theory did not change breeding practices directly. Instead, breeding became more orderly as a consequence of pure line theory, which structured breeding programmes and eliminated external heritable influences. This incremental change then explains how and why the large multi-national seed companies that we know today were created; pure lines invited standardisation and economies of scale that the latter were designed to exploit. Rather than focus on breeding practice, this paper examines the plant varietal market itself. It focusses upon work conducted by the National Institute of Agricultural Botany (NIAB) during the interwar years, and in doing so demonstrates that, on the contrary, the pure line was actually only partially accepted by the industry. Moreover, claims that contradicted the logic of the pure line were not merely tolerated by the agricultural geneticists affiliated with NIAB, but were acknowledged and legitimised by them. The history of how and why the plant breeding industry was transformed remains to be written. PMID:24650856

  15. Towards personalized agriculture: what chemical genomics can bring to plant biotechnology

    PubMed Central

    Stokes, Michael E.; McCourt, Peter

    2014-01-01

    In contrast to the dominant drug paradigm in which compounds were developed to “fit all,” new models focused around personalized medicine are appearing in which treatments are developed and customized for individual patients. The agricultural biotechnology industry (Ag-biotech) should also think about these new personalized models. For example, most common herbicides are generic in action, which led to the development of genetically modified crops to add specificity. The ease and accessibility of modern genomic analysis, when wedded to accessible large chemical space, should facilitate the discovery of chemicals that are more selective in their utility. Is it possible to develop species-selective herbicides and growth regulators? More generally put, is plant research at a stage where chemicals can be developed that streamline plant development and growth to various environments? We believe the advent of chemical genomics now opens up these and other opportunities to “personalize” agriculture. Furthermore, chemical genomics does not necessarily require genetically tractable plant models, which in principle should allow quick translation to practical applications. For this to happen, however, will require collaboration between the Ag-biotech industry and academic labs for early stage research and development, a situation that has proven very fruitful for Big Pharma. PMID:25183965

  16. Biogas production from rice straw by solid-state anaerobic digestion

    NASA Astrophysics Data System (ADS)

    Shitophyta, Lukhi Mulia; Budiyono, Fuadi, Ahmad M.

    2015-12-01

    Biogas production from lignocellulosic biomass can be used as an alternative fuel to replace fossil fuels. Lignocellulose can be obtained from agricultural crop residues, such as rice straw. The aims of this study were to determine the effects of F/I ratio, total solid content, and physical pretreatment on biogas production by solid-state anaerobic digestion. The kinetics of biogas production were also examined in this study. The results showed that the biogas yield decreased by the increasing of F/I ratio. Meanwhile, the increase TS content of 22% to 24% also decreased the biogas yield. Physical pretreatment had no a significant effect on biogas yield (p > 0.05). The highest biogas yield of 248.4 L/kg VS was obtained at an F/I ratio of 2, TS content of 22%, and particle size of 2 mm. The kinetics of biogas production from rice straw followed the first-order kinetic model with the highest rate constant (k) of 0.0861 day-1.

  17. Historic Mining and Agriculture as Indicators of Occurrence and Abundance of Widespread Invasive Plant Species

    PubMed Central

    Calinger, Kellen; Calhoon, Elisabeth; Chang, Hsiao-chi; Whitacre, James; Wenzel, John; Comita, Liza; Queenborough, Simon

    2015-01-01

    Anthropogenic disturbances often change ecological communities and provide opportunities for non-native species invasion. Understanding the impacts of disturbances on species invasion is therefore crucial for invasive species management. We used generalized linear mixed effects models to explore the influence of land-use history and distance to roads on the occurrence and abundance of two invasive plant species (Rosa multiflora and Berberis thunbergii) in a 900-ha deciduous forest in the eastern U.S.A., the Powdermill Nature Reserve. Although much of the reserve has been continuously forested since at least 1939, aerial photos revealed a variety of land-uses since then including agriculture, mining, logging, and development. By 2008, both R. multiflora and B. thunbergii were widespread throughout the reserve (occurring in 24% and 13% of 4417 10-m diameter regularly-placed vegetation plots, respectively) with occurrence and abundance of each varying significantly with land-use history. Rosa multiflora was more likely to occur in historically farmed, mined, logged or developed plots than in plots that remained forested, (log odds of 1.8 to 3.0); Berberis thunbergii was more likely to occur in plots with agricultural, mining, or logging history than in plots without disturbance (log odds of 1.4 to 2.1). Mining, logging, and agriculture increased the probability that R. multiflora had >10% cover while only past agriculture was related to cover of B. thunbergii. Proximity to roads was positively correlated with the occurrence of R. multiflora (a 0.26 increase in the log odds for every 1-m closer) but not B. thunbergii, and roads had no impact on the abundance of either species. Our results indicated that a wide variety of disturbances may aid the introduction of invasive species into new habitats, while high-impact disturbances such as agriculture and mining increase the likelihood of high abundance post-introduction. PMID:26046534

  18. Historic Mining and Agriculture as Indicators of Occurrence and Abundance of Widespread Invasive Plant Species.

    PubMed

    Calinger, Kellen; Calhoon, Elisabeth; Chang, Hsiao-Chi; Whitacre, James; Wenzel, John; Comita, Liza; Queenborough, Simon

    2015-01-01

    Anthropogenic disturbances often change ecological communities and provide opportunities for non-native species invasion. Understanding the impacts of disturbances on species invasion is therefore crucial for invasive species management. We used generalized linear mixed effects models to explore the influence of land-use history and distance to roads on the occurrence and abundance of two invasive plant species (Rosa multiflora and Berberis thunbergii) in a 900-ha deciduous forest in the eastern U.S.A., the Powdermill Nature Reserve. Although much of the reserve has been continuously forested since at least 1939, aerial photos revealed a variety of land-uses since then including agriculture, mining, logging, and development. By 2008, both R. multiflora and B. thunbergii were widespread throughout the reserve (occurring in 24% and 13% of 4417 10-m diameter regularly-placed vegetation plots, respectively) with occurrence and abundance of each varying significantly with land-use history. Rosa multiflora was more likely to occur in historically farmed, mined, logged or developed plots than in plots that remained forested, (log odds of 1.8 to 3.0); Berberis thunbergii was more likely to occur in plots with agricultural, mining, or logging history than in plots without disturbance (log odds of 1.4 to 2.1). Mining, logging, and agriculture increased the probability that R. multiflora had >10% cover while only past agriculture was related to cover of B. thunbergii. Proximity to roads was positively correlated with the occurrence of R. multiflora (a 0.26 increase in the log odds for every 1-m closer) but not B. thunbergii, and roads had no impact on the abundance of either species. Our results indicated that a wide variety of disturbances may aid the introduction of invasive species into new habitats, while high-impact disturbances such as agriculture and mining increase the likelihood of high abundance post-introduction. PMID:26046534

  19. Dielectric spectroscopic studies on the water hyacinth plant collected from agriculture drainage.

    PubMed

    Mahani, Ragab; Atia, Fatma; Al Neklawy, Mohammed M; Fahem, Amin

    2016-06-01

    The present paper aims to investigate the sensitivity of dielectric spectroscopy to changes in concentrations of pollutants (heavy metals and metal oxides) uptake by the water hyacinth plant collected from agriculture wastewater drainage. The measurements were carried out on the dried root and shoot plant parts before and after subjecting to different microwave heating powers for different times. Dielectric properties of the untreated root were investigated at temperature range (30-90°C). X-ray fluorescence spectroscopy (XRF) results showed that the concentration of metals and metals oxides are higher in plant root than in plant shoot. Accordingly, the obtained dielectric properties were found to depend on the applied electric field frequency, magnitude of heating power as well as concentrations of pollutants. Analysis of experimental data represented by the imaginary part of the dielectric modulus M″ (ω) revealed to the presence of three different relaxation processes. The lower frequency relaxation process was associated to charge carriers conduction whereas those appeared at higher frequencies were associated to different types of interfacial polarization. The plant ability for removing heavy metals and metal oxides from the aquatic environments would be enhanced upon subjecting to microwave heating power with 400 W for 30 min. PMID:26985876

  20. Dielectric spectroscopic studies on the water hyacinth plant collected from agriculture drainage

    NASA Astrophysics Data System (ADS)

    Mahani, Ragab; Atia, Fatma; Al Neklawy, Mohammed M.; Fahem, Amin

    2016-06-01

    The present paper aims to investigate the sensitivity of dielectric spectroscopy to changes in concentrations of pollutants (heavy metals and metal oxides) uptake by the water hyacinth plant collected from agriculture wastewater drainage. The measurements were carried out on the dried root and shoot plant parts before and after subjecting to different microwave heating powers for different times. Dielectric properties of the untreated root were investigated at temperature range (30-90 °C). X-ray fluorescence spectroscopy (XRF) results showed that the concentration of metals and metals oxides are higher in plant root than in plant shoot. Accordingly, the obtained dielectric properties were found to depend on the applied electric field frequency, magnitude of heating power as well as concentrations of pollutants. Analysis of experimental data represented by the imaginary part of the dielectric modulus M″ (ω) revealed to the presence of three different relaxation processes. The lower frequency relaxation process was associated to charge carriers conduction whereas those appeared at higher frequencies were associated to different types of interfacial polarization. The plant ability for removing heavy metals and metal oxides from the aquatic environments would be enhanced upon subjecting to microwave heating power with 400 W for 30 min.

  1. Effects of coal-fired thermal power plant discharges on agricultural soil and crop plants

    SciTech Connect

    Ajmal, M.; Khan, M.A.

    1986-04-01

    The physicochemical properties of the upstream and downstream waters from the Upper Ganga canal, discharged cooling tower water, machine washings, and scrubber and bottom ash effluents of a 530 MW Kasimpur coal-fired thermal power plant have been determined, and their effects directly on fertile soil and indirectly on pea (Pisum sativam) and wheat (Triticum aestivum) crops have also been studied. The effluents were alkaline in nature. The scrubber and bottom ash effluent contained large amounts of solids and had high biochemical and chemical oxygen demands. The soils irrigated with the different effluents exhibited an increase in pH, organic matter, calcium carbonate, water-soluble salts, cation exchange capacity, electrical conductivity, and nitrogen and phosphorus contents while potassium content decreased. The effects of 100, 50, and 0% (tap water control) dilutions of cooling tower, machine washings, and scrubber and bottom ash effluents on the germination and growth of pea and wheat crops were also monitored. Using the undiluted effluents, there was 100% germination for both crops when irrigation was done with cooling tower effluent. Germination was restricted to 90% for the two crops when irrigated with machine washings effluent, and to 80 and 70% for pea and wheat, respectively, when irrigated with scrubber and bottom ash effluent. Samples of upstream and downstream canal water were also used for irrigating soils with and without crop plants in order to ascertain the impact of effluents on canal water and its subsequent effect on crops. The soils irrigated with downstream canal water were found to contain slightly more calcium carbonate, phosphorus, and ammonia-nitrogen than those receiving upstream canal water. Though 100% germination was obtained in both cases, the growth of plants irrigated with the downstream canal water was slightly reduced.

  2. Occurrence of an herbicide-resistant plant trait in agricultural field margins.

    PubMed

    Gage, Karla L; Gibson, David J; Young, Bryan G; Young, Julie M; Matthews, Joseph L; Weller, Stephen C; Wilson, Robert G

    2015-09-01

    Agricultural environments allow study of evolutionary change in plants. An example of evolution within agroecological systems is the selection for resistance to the herbicide glyphosate within the weed, Conyza canadensis. Changes in survivorship and reproduction associated with the development of glyphosate resistance (GR) may impact fitness and influence the frequency of occurrence of the GR trait. We hypothesized that site characteristics and history would affect the occurrence of GR C. canadensis in field margins. We surveyed GR occurrence in field margins and asked whether there were correlations between GR occurrence and location, crop rotation, GR crop trait rotation, crop type, use of tillage, and the diversity of herbicides used. In a field experiment, we hypothesized that there would be no difference in fitness between GR and glyphosate-susceptible (GS) plants. We asked whether there were differences in survivorship, phenology, reproduction, and herbivory between 2 GR and 2 GS populations of C. canadensis in agrestal and ruderal habitats. We found that geographic location was an important factor in the occurrence of GR C. canadensis in field margins. Although not consistently associated with either glyphosate resistance or glyphosate susceptibility, there were differences in phenology, survivorship, and herbivory among biotypes of C. canadensis. We found equal or greater fitness in GR biotypes, compared to GS biotypes, and GR plants were present in field margins. Field margins or ruderal habitats may provide refugia for GR C. canadensis, allowing reproduction and further selection to occur as seeds recolonize the agrestal habitat. Agricultural practices may select for ecological changes that feed back into the evolution of plants in ruderal habitats. PMID:26445665

  3. Agricultural, domestic and handicraft folk uses of plants in the Tyrrhenian sector of Basilicata (Italy)

    PubMed Central

    Salerno, Giovanni; Guarrera, Paolo Maria; Caneva, Giulia

    2005-01-01

    Background Research was carried out into agricultural and domestic-handicraft uses in folk traditions in the Tyrrhenian sector of the Basilicata region (southern Italy), as it is typically representative of ethnobotanical applications in the Mediterranean area. From the point of view of furnishing a botanical support for the study of local "material culture" data was collected through field interviews of 49 informants, most of whom were farmers. Results The taxa cited are 60, belonging to 32 botanical families, of which 18 are employed for agricultural uses and 51 for domestic-handicraft folk uses. Data show a diffuse use of plants for many purposes, both in agricultural (present uses 14%; past uses 1%) and for domestic-handicraft use (present uses 40%; past uses 45%); most of the latter are now in decline. Conclusion 60 data look uncommon or typical of the places studied. Some domestic-handicraft folk uses are typical of southern Italy (e.g. the use of Ampelodesmos mauritanicus for making ties, ropes, torches, baskets or that of Acer neapolitanum for several uses). Other uses (e.g. that of Inula viscosa and Calamintha nepeta for peculiar brooms, and of Origanum heracleoticum for dyeing wool red) are previously unpublished. PMID:16270919

  4. Noncrop flowering plants restore top-down herbivore control in agricultural fields

    PubMed Central

    Balmer, Oliver; Pfiffner, Lukas; Schied, Johannes; Willareth, Martin; Leimgruber, Andrea; Luka, Henryk; Traugott, Michael

    2013-01-01

    Abstract Herbivore populations are regulated by bottom-up control through food availability and quality and by top-down control through natural enemies. Intensive agricultural monocultures provide abundant food to specialized herbivores and at the same time negatively impact natural enemies because monocultures are depauperate in carbohydrate food sources required by many natural enemies. As a consequence, herbivores are released from both types of control. Diversifying intensive cropping systems with flowering plants that provide nutritional resources to natural enemies may enhance top-down control and contribute to natural herbivore regulation. We analyzed how noncrop flowering plants planted as “companion plants” inside cabbage (Brassica oleracea) fields and as margins along the fields affect the plant–herbivore–parasitoid–predator food web. We combined molecular analyses quantifying parasitism of herbivore eggs and larvae with molecular predator gut content analysis and a comprehensive predator community assessment. Planting cornflowers (Centaurea cynanus), which have been shown to attract and selectively benefit Microplitis mediator, a larval parasitoid of the cabbage moth Mamestra brassicae, between the cabbage heads shifted the balance between trophic levels. Companion plants significantly increased parasitism of herbivores by larval parasitoids and predation on herbivore eggs. They furthermore significantly affected predator species richness. These effects were present despite the different treatments being close relative to the parasitoids’ mobility. These findings demonstrate that habitat manipulation can restore top-down herbivore control in intensive crops if the right resources are added. This is important because increased natural control reduces the need for pesticide input in intensive agricultural settings, with cascading positive effects on general biodiversity and the environment. Companion plants thus increase biodiversity both

  5. Combined hydrogen and carbon isotopes of plant waxes as an indicator of drought impacts on ancient Maya agriculture

    NASA Astrophysics Data System (ADS)

    Douglas, P. M.; Pagani, M.; Eglinton, T. I.; Brenner, M.; Hodell, D. A.; Curtis, J. H.

    2012-12-01

    There is increasing evidence suggesting that a series of droughts in the Yucatan Peninsula coincided with the Terminal Classic decline of the Classic Maya civilization (ca. 1250 to 1000 years BP). However, there is little evidence directly linking climatic change and changes in human activities in this region. In this study we combine plant-wax δD, δ13C, and Δ14C analyses in two lake sediment cores from southeastern Mexico and northern Guatemala to develop coupled records of hydroclimate variability and human-driven vegetation change. Plant-wax specific Δ14C ages indicate a large input of pre-aged plant waxes into lake sediment. Comparison of plant-wax δD records with other regional hydroclimate proxy records suggest that plant-wax ages are evenly distributed around plant-wax radiocarbon ages, and that applying an age model based on plant-wax radiocarbon ages is appropriate for these lake sediments. We evaluate how differences in plant-wax age distributions influence stable isotope records to assess the age uncertainty associated with records of climate and vegetation change derived from plant-wax stable isotopes. In this low-elevation tropical environment plant-wax δ13C is largely controlled by the relative abundance of C3 and C4 plants. The ancient Maya practiced widespread maize (C4) agriculture and strongly influenced regional C3-C4 vegetation dynamics. Under natural conditions C4 plant coverage and plant-wax δ13C would tend to co-vary positively since C4 plants are well adapted for dry conditions. Under ancient Maya land-use, however, this relationship is likely to be decoupled, since drought would have disrupted C4 agriculture. Combined analysis of plant-wax δD and δ13C from both lakes indicates increasingly divergent trends following ca. 3500 years BP, around the onset of widespread ancient Maya agriculture. After this time high plant-wax δD values tend to correspond with low plant-wax δ13C values and vice versa. This pattern is consistent with

  6. Biogas and Fuel Cells Workshop Summary Report: Proceedings from the Biogas and Fuel Cells Workshop, Golden, Colorado, June 11-13, 2012

    SciTech Connect

    Not Available

    2013-01-01

    The U.S. Department of Energy (DOE) National Renewable Energy Laboratory (NREL) held a Biogas and Fuel Cells Workshop June 11-13, 2012, in Golden, Colorado, to discuss biogas and waste-to-energy technologies for fuel cell applications. The overall objective was to identify opportunities for coupling renewable biomethane with highly efficient fuel cells to produce electricity; heat; combined heat and power (CHP); or combined heat, hydrogen and power (CHHP) for stationary or motive applications. The workshop focused on biogas sourced from wastewater treatment plants (WWTPs), landfills, and industrial facilities that generate or process large amounts of organic waste, including large biofuel production facilities (biorefineries).

  7. Agriculture in the developing world: Connecting innovations in plant research to downstream applications.

    PubMed

    Delmer, Deborah P

    2005-11-01

    Enhancing agricultural productivity in those areas of the world bypassed by the Green Revolution will require new approaches that provide incentives and funding mechanisms that promote the translation of new innovations in plant science into concrete benefits for poor farmers. Through better dialogue, plant breeders and laboratory scientists from both the public and private-sectors need to find solutions for the key constraints to crop production, many of which center around abiotic and biotic stresses. The revolution in plant genomics has opened up new perspectives and opportunities for plant breeders who can now apply molecular markers to assess and enhance diversity in their germplasm collections, to introgress valuable traits from new sources, and to identify genes that control key traits. Functional genomics is also providing another powerful route to the identification of such genes. The ability to introgress beneficial genes under the control of specific promoters through transgenic approaches is yet one more stepping stone in the path to targeted approaches to crop improvement, and the new sciences have identified a vast array of genes that have exciting potential for crop improvement. For a few crops with viable markets, such as maize and cotton, some of the traits developed by the private sector are already showing benefits for farmers of the developing world, but the public sector will need to develop new skills and overcome a number of hurdles to carry out similar efforts for other crops and traits useful to very poor farmers. PMID:16263937

  8. Effects of cyanobacteria producing microcystins on seed germination and seedling growth of several agricultural plants.

    PubMed

    Saqrane, Sana; El Ghazali, Issam; Oudra, Brahim; Bouarab, L; Vasconcelos, Vitor

    2008-06-01

    The effects of cyanobacteria aqueous extracts containing Microcystin-LR (MC-LR) on the seed germination and growth of Pisum sativum, Lens esculenta, Zea mays and Triticum durum were investigated. Experiments were carried out on a range of doses of the extract (equivalent to 0, 1.6, 2.9, 5.8, 8.7 and 11.6 mu g MC-LR/mL). The results confirm that these plants were sensitive to cell-free extracts of a toxic Microcystis and that germination inhibition was dose dependent. One-way analysis of variance (ANOVA) showed that P. sativum is the most sensitive tested species with a 97% germination rate reduction and L. esculenta was the most resistant. At the 8th day, the exposure to the microcystins (MC) resulted in a significant decrease of plant epicotyls length, roots length and a net inhibition of lateral root formation. It is concluded that MC could affect also terrestrial plants seedling germination and growth. Therefore, the use of water for irrigation contaminated by MC could exert negative biochemical effects on seed and plant metabolism which might influence the agricultural crops. PMID:18576226

  9. Agriculture in the developing world: Connecting innovations in plant research to downstream applications

    PubMed Central

    Delmer, Deborah P.

    2005-01-01

    Enhancing agricultural productivity in those areas of the world bypassed by the Green Revolution will require new approaches that provide incentives and funding mechanisms that promote the translation of new innovations in plant science into concrete benefits for poor farmers. Through better dialogue, plant breeders and laboratory scientists from both the public and private-sectors need to find solutions for the key constraints to crop production, many of which center around abiotic and biotic stresses. The revolution in plant genomics has opened up new perspectives and opportunities for plant breeders who can now apply molecular markers to assess and enhance diversity in their germplasm collections, to introgress valuable traits from new sources, and to identify genes that control key traits. Functional genomics is also providing another powerful route to the identification of such genes. The ability to introgress beneficial genes under the control of specific promoters through transgenic approaches is yet one more stepping stone in the path to targeted approaches to crop improvement, and the new sciences have identified a vast array of genes that have exciting potential for crop improvement. For a few crops with viable markets, such as maize and cotton, some of the traits developed by the private sector are already showing benefits for farmers of the developing world, but the public sector will need to develop new skills and overcome a number of hurdles to carry out similar efforts for other crops and traits useful to very poor farmers. PMID:16263937

  10. Treatment of digestate from a co-digestion biogas plant by means of vacuum evaporation: tests for process optimization and environmental sustainability.

    PubMed

    Chiumenti, A; da Borso, F; Chiumenti, R; Teri, F; Segantin, P

    2013-06-01

    Vacuum evaporation consists in the boiling of a liquid substrate at negative pressure, at a temperature lower than typical boiling temperature at atmospheric conditions. Condensed vapor represents the so called condensate, while the remaining substrate represents the concentrate. This technology is derived from other sectors and is mainly dedicated to the recovery of chemicals from industrial by-products, while it has not been widely implemented yet in the field of agricultural digestate treatment. The present paper relates on experimental tests performed in pilot-scale vacuum evaporation plants (0.100 and 0.025 m(3)), treating filtered digestate (liquid fraction of digestate filtered by a screw-press separator). Digestate was produced by a 1 MWe anaerobic digestion plant fed with swine manure, corn silage and other biomasses. Different system and process configurations were tested (single-stage and two-stage, with and without acidification) with the main objectives of assessing the technical feasibility and of optimizing process parameters for an eventual technology transfer to full scale systems. The inputs and outputs of the process were subject to characterization and mass and nutrients balances were determined. The vacuum evaporation process determined a relevant mass reduction of digestate. The single stage configuration determined the production of a concentrate, still in liquid phase, with a total solid (TS) mean concentration of 15.0%, representing, in terms of mass, 20.2% of the input; the remaining 79.8% was represented by condensate. The introduction of the second stage allowed to obtain a solid concentrate, characterized by a content of TS of 59.0% and representing 5.6% of initial mass. Nitrogen balance was influenced by digestate pH: in order to limit the stripping of ammonia and its transfer to condensate it was necessary to reduce the pH. At pH 5, 97.5% of total nitrogen remained in the concentrate. This product was characterized by very high

  11. Tightly-Coupled Plant-Soil Nitrogen Cycling: Comparison of Organic Farms across an Agricultural Landscape.

    PubMed

    Bowles, Timothy M; Hollander, Allan D; Steenwerth, Kerri; Jackson, Louise E

    2015-01-01

    How farming systems supply sufficient nitrogen (N) for high yields but with reduced N losses is a central challenge for reducing the tradeoffs often associated with N cycling in agriculture. Variability in soil organic matter and management of organic farms across an agricultural landscape may yield insights for improving N cycling and for evaluating novel indicators of N availability. We assessed yields, plant-soil N cycling, and root expression of N metabolism genes across a representative set of organic fields growing Roma-type tomatoes (Solanum lycopersicum L.) in an intensively-managed agricultural landscape in California, USA. The fields spanned a three-fold range of soil carbon (C) and N but had similar soil types, texture, and pH. Organic tomato yields ranged from 22.9 to 120.1 Mg ha-1 with a mean similar to the county average (86.1 Mg ha-1), which included mostly conventionally-grown tomatoes. Substantial variability in soil inorganic N concentrations, tomato N, and root gene expression indicated a range of possible tradeoffs between yields and potential for N losses across the fields. Fields showing evidence of tightly-coupled plant-soil N cycling, a desirable scenario in which high crop yields are supported by adequate N availability but low potential for N loss, had the highest total and labile soil C and N and received organic matter inputs with a range of N availability. In these fields, elevated expression of a key gene involved in root N assimilation, cytosolic glutamine synthetase GS1, confirmed that plant N assimilation was high even when inorganic N pools were low. Thus tightly-coupled N cycling occurred on several working organic farms. Novel combinations of N cycling indicators (i.e. inorganic N along with soil microbial activity and root gene expression for N assimilation) would support adaptive management for improved N cycling on organic as well as conventional farms, especially when plant-soil N cycling is rapid. PMID:26121264

  12. Tightly-Coupled Plant-Soil Nitrogen Cycling: Comparison of Organic Farms across an Agricultural Landscape

    PubMed Central

    Bowles, Timothy M.; Hollander, Allan D.; Steenwerth, Kerri; Jackson, Louise E.

    2015-01-01

    How farming systems supply sufficient nitrogen (N) for high yields but with reduced N losses is a central challenge for reducing the tradeoffs often associated with N cycling in agriculture. Variability in soil organic matter and management of organic farms across an agricultural landscape may yield insights for improving N cycling and for evaluating novel indicators of N availability. We assessed yields, plant-soil N cycling, and root expression of N metabolism genes across a representative set of organic fields growing Roma-type tomatoes (Solanum lycopersicum L.) in an intensively-managed agricultural landscape in California, USA. The fields spanned a three-fold range of soil carbon (C) and N but had similar soil types, texture, and pH. Organic tomato yields ranged from 22.9 to 120.1 Mg ha-1 with a mean similar to the county average (86.1 Mg ha-1), which included mostly conventionally-grown tomatoes. Substantial variability in soil inorganic N concentrations, tomato N, and root gene expression indicated a range of possible tradeoffs between yields and potential for N losses across the fields. Fields showing evidence of tightly-coupled plant-soil N cycling, a desirable scenario in which high crop yields are supported by adequate N availability but low potential for N loss, had the highest total and labile soil C and N and received organic matter inputs with a range of N availability. In these fields, elevated expression of a key gene involved in root N assimilation, cytosolic glutamine synthetase GS1, confirmed that plant N assimilation was high even when inorganic N pools were low. Thus tightly-coupled N cycling occurred on several working organic farms. Novel combinations of N cycling indicators (i.e. inorganic N along with soil microbial activity and root gene expression for N assimilation) would support adaptive management for improved N cycling on organic as well as conventional farms, especially when plant-soil N cycling is rapid. PMID:26121264

  13. The total and freely dissolved polycyclic aromatic hydrocarbons content in residues from biogas production.

    PubMed

    Stefaniuk, Magdalena; Oleszczuk, Patryk

    2016-01-01

    In the situation of increasing agricultural utilization of residues from biogas production (RBP) it is important to determine the concentration of contaminants, which could occur in these materials. The group of contaminants that requires special attention are polycyclic aromatic hydrocarbons (PAH). The objective of the study was to determine the total and freely dissolved (Cfree) of PAHs in RBP from 6 different biogas plants operating under various temperature conditions and without or with the separation into the solid and liquid fractions. The freely dissolved PAHs were determined using polyoxymethylene (POM method). The total content of the Σ16 PAHs in RBP varied from 449 to 6147 μg/kgdw, while that of Cfree PAHs was at the level from 57 to 653 ng/L. No significant differences were noted in the content of the Σ16 PAHs (total) between the solid and the liquid fractions. This indicates that in the course of the separation, the PAHs are distributed proportionally between the fractions. However in the case of Cfree, PAHs content in the solid fraction was over twice as high as in the liquid fraction. This was probably due to the greater affinity of the particles present in the liquid fraction to the analysed PAHs than to the particles of the solid fraction. Higher affinity to liquid fraction was also confirmed by the distribution coefficients KTOC determined on the basis of Cfree. PMID:26586628

  14. Evaluation of the micronutrient composition of plant foods produced by organic and conventional agricultural methods.

    PubMed

    Hunter, Duncan; Foster, Meika; McArthur, Jennifer O; Ojha, Rachel; Petocz, Peter; Samman, Samir

    2011-07-01

    The aim of the present analysis was to evaluate the micronutrient content of plant foods produced by organic and conventional agricultural methods. Studies were identified from a search of electronic databases (1980-2007, inclusive) as well as manual searches. A total of 66 studies (describing 1440 micronutrient comparisons) were identified. Thirty-three studies (908 comparisons) satisfied the screening criteria which considered cultivar, harvesting, and soil conditions. In studies that satisfied the screening criteria, the absolute levels of micronutrients were higher in organic foods more often than in conventional foods (462 vs 364 comparisons, P=0.002), and the total micronutrient content, expressed as a percent difference, was higher in organic (+5.7%, P<0.001) as compared to conventionally grown produce. The micronutrient content of food groups was more frequently reported to be higher for organic vegetables and legumes compared to their conventional counterparts (vegetables, 267 vs 197, P<0.001; legumes, 79 vs 46, P=0.004). This trend was supported by a mean percent difference in micronutrient content favoring organic vegetables (+5.9%, P<0.001) and legumes (+5.7%, P<0.001). Further research is required to determine the effect of organic agricultural methods on a broader range of nutrients and their potential impact on health. PMID:21929333

  15. Assessing health in agriculture--towards a common research framework for soils, plants, animals, humans and ecosystems.

    PubMed

    Vieweger, Anja; Döring, Thomas F

    2015-02-01

    In agriculture and food systems, health-related research includes a vast diversity of topics. Nutritional, toxicological, pharmacological, epidemiological, behavioural, sociological, economic and political methods are used to study health in the five domains of soils, plants, livestock, humans and ecosystems. An idea developed in the early founding days of organic agriculture stated that the health of all domains is one and indivisible. Here we show that recent research reveals the existence and complex nature of such health links among domains. However, studies of health aspects in agriculture are often separated by disciplinary boundaries. This restrains the understanding of health in agricultural systems. Therefore we explore the opportunities and limitations of bringing perspectives together from the different domains. We review current approaches to define and assess health in agricultural contexts, comparing the state of the art of commonly used approaches and bringing together the presently disconnected debates in soil science, plant science, veterinary science and human medicine. Based on a qualitative literature analysis, we suggest that many health criteria fall into two paradigms: (1) the Growth Paradigm, where terms are primarily oriented towards continued growth; (2) the Boundary Paradigm, where terms focus on maintaining or coming back to a status quo, recognising system boundaries. Scientific health assessments in agricultural and food systems need to be explicit in terms of their position on the continuum between Growth Paradigm and Boundary Paradigm. Finally, we identify areas and concepts for a future direction of health assessment and research in agricultural and food systems. PMID:24777948

  16. Organic Farming Benefits Local Plant Diversity in Vineyard Farms Located in Intensive Agricultural Landscapes

    NASA Astrophysics Data System (ADS)

    Nascimbene, Juri; Marini, Lorenzo; Paoletti, Maurizio G.

    2012-05-01

    The majority of research on organic farming has considered arable and grassland farming systems in Central and Northern Europe, whilst only a few studies have been carried out in Mediterranean agro-systems, such as vineyards, despite their economic importance. The main aim of the study was to test whether organic farming enhances local plant species richness in both crop and non-crop areas of vineyard farms located in intensive conventional landscapes. Nine conventional and nine organic farms were selected in an intensively cultivated region (i.e. no gradient in landscape composition) in northern Italy. In each farm, vascular plants were sampled in one vineyard and in two non-crop linear habitats, grass strips and hedgerows, adjacent to vineyards and therefore potentially influenced by farming. We used linear mixed models to test the effect of farming, and species longevity (annual vs. perennial) separately for the three habitat types. In our intensive agricultural landscapes organic farming promoted local plant species richness in vineyard fields, and grassland strips while we found no effect for linear hedgerows. Differences in species richness were not associated to differences in species composition, indicating that similar plant communities were hosted in vineyard farms independently of the management type. This negative effect of conventional farming was probably due to the use of herbicides, while mechanical operations and mowing regime did not differ between organic and conventional farms. In grassland strips, and only marginally in vineyards, we found that the positive effect of organic farming was more pronounced for perennial than annual species.

  17. Production costs and operative margins in electric energy generation from biogas. Full-scale case studies in Italy.

    PubMed

    Riva, C; Schievano, A; D'Imporzano, G; Adani, F

    2014-08-01

    The purpose of this study was to observe the economic sustainability of three different biogas full scale plants, fed with different organic matrices: energy crops (EC), manure, agro-industrial (Plants B and C) and organic fraction of municipal solid waste (OFMSW) (Plant A). The plants were observed for one year and total annual biomass feeding, biomass composition and biomass cost (€ Mg(-1)), initial investment cost and plant electric power production were registered. The unit costs of biogas and electric energy (€ Sm(-3)biogas, € kWh(-1)EE) were differently distributed, depending on the type of feed and plant. Plant A showed high management/maintenance cost for OFMSW treatment (0.155 € Sm(-3)biogas, 45% of total cost), Plant B suffered high cost for EC supply (0.130 € Sm(-3)biogas, 49% of total cost) and Plant C showed higher impact on the total costs because of the depreciation charge (0.146 € Sm(-3)biogas, 41% of total costs). The breakeven point for the tariff of electric energy, calculated for the different cases, resulted in the range 120-170 € MWh(-1)EE, depending on fed materials and plant scale. EC had great impact on biomass supply costs and should be reduced, in favor of organic waste and residues; plant scale still heavily influences the production costs. The EU States should drive incentives in dependence of these factors, to further develop this still promising sector. PMID:24841069

  18. PLANT SENESCENCE: A MECHANISM FOR NUTRIENT RELEASE IN TEMPERATE AGRICULTURAL WETLANDS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Agriculture drainage ditches are primary intercept wetlands in amelioration of nutrient pollution from agricultural fields. Drainage ditches, as integral components of the agricultural landscape, remove surface run-off and act as major conduits of nutrients from agricultural lands to receiving water...

  19. Agricultural Microbiology.

    ERIC Educational Resources Information Center

    Brill, Winston J.

    1981-01-01

    Elucidates strategies for applying microbiological techniques to traditional agricultural practices. Discusses the manipulation of microorganisms that live with plants and also the problems involved in the introduction of new genes into crop plants by recombinant DNA methods. (CS)

  20. Pilot-scale field study for ammonia removal from lagoon biogas using an acid wet scrubber.

    PubMed

    Lin, Hongjian; Wu, Xiao; Miller, Curtis; Zhu, Jun; Hadlocon, Lara Jane; Manuzon, Roderick; Zhao, Lingying

    2014-01-01

    The anaerobic activities in swine slurry storage and treatment generate biogas containing gaseous ammonia component which is a chemical agent that can cause adverse environmental impacts when released to the atmosphere. The aim of this pilot plant study was to remove ammonia from biogas generated in a covered lagoon, using a sulfuric acid wet scrubber. The data showed that, on average, the biogas contained 43.7 ppm of ammonia and its concentration was found to be exponentially related to the air temperature inside the lagoon. When the air temperature rose to 35°C and the biogas ammonia concentration reached 90 ppm, the mass transfer of ammonia/ammonium from the deeper liquid body to the interface between the air and liquid became a limiting factor. The biogas velocity was critical in affecting ammonia removal efficiency of the wet scrubber. A biogas flow velocity of 8 to 12 mm s(-1) was recommended to achieve a removal efficiency of greater than 60%. Stepwise regression revealed that the biogas velocity and air temperature, not the inlet ammonia concentration in biogas, affected the ammonia removal efficiency. Overall, when 73 g L(-1) (or 0.75 M) sulfuric acid solution was used as the scrubber solution, removal efficiencies varied from 0% to 100% with an average of 55% over a 40-d measurement period. Mass balance calculation based on ammonium-nitrogen concentration in final scrubber liquid showed that about 21.3 g of ammonia was collected from a total volume of 1169 m(3) of biogas, while the scrubber solution should still maintain its ammonia absorbing ability until its concentration reaches up to 1 M. These results showed promising use of sulfuric acid wet scrubber for ammonia removal in the digester biogas. PMID:24762182

  1. Introduction to biogas production on the farm

    SciTech Connect

    Not Available

    1984-03-01

    A number of farmers, ranchers, and engineers received support from the US Department of Energy Appropriate Technology Small Grants Program to design, construct, and demonstrate biogas production systems. Many of these projects generated more than just biogas; grantees' work and results have contributed to a growing body of information about practical applications of this technology. This publication was developed to share some of that information, to answer the basic questions about biogas production, and to lead farmers to more information. Section I introduces biogas and the various components of a biogas production system, discusses the system's benefits and liabilities, and provides a brief checklist to determine if biogas production may be applicable to an individual's particular situation. Section II features descriptions of four biogas projects of various sizes. Section III provides sources of additional information including descriptions of other biogas production projects.

  2. Perry`s bio-gas experience 1995 ASME/EPRI radwaste workshop

    SciTech Connect

    Schwenk, A.K.

    1995-09-01

    The Perry Power Plant has been in commercial operation for about ten years. Although we didn`t know it at the time, we now believe our bio-gas problem may have started about seven years ago. Barnwell discovered we had a bio-gas problem about a year and a half ago. We found out we had a bio-gas problem a few hours later. The history associated with this process at Perry is outlined, and past as well as present efforts to monitor this process are also discussed.

  3. Agricultural approaches of remediation in the outside of the Fukushima Daiichi nuclear power plant

    SciTech Connect

    Sato, Nobuaki; Saso, Michitaka; Umeda, Miki; Fujii, Yasuhiko; Amemiya, Kiyoshi

    2013-07-01

    This paper outlines agricultural approaches of remediation activity done in contaminated areas around the Fukushima Daiichi Nuclear Power Plant. About the decontamination examination of contaminated areas, we have tried the land scale test of a rice field before and after planting by the use of currently recommended methods. Since farmers would carry out the land preparation by themselves, generation of secondary radioactive waste should be as low as possible through the decontamination works. For the radioactive nuclide migration control of rice by wet rice production, several types of decontamination methods such as zeolite addition and potassium fertilization in the soil have been examined. The results are summarized in the 4 following points. 1) Plowing and water discharge are effective for removing radioactive cesium from rice field. 2) Additional potassium fertilization is effective for reducing cesium radioactivity in the product. 3) No significant difference is observed with or without the zeolite addition. 4) Very low transfer factor of cesium from soil to brown rice has been obtained compared with literature values.

  4. Greenhouse gas emissions of an agro-biogas energy system: Estimation under the Renewable Energy Directive.

    PubMed

    Rana, Roberto; Ingrao, Carlo; Lombardi, Mariarosaria; Tricase, Caterina

    2016-04-15

    Agro-biogas from energy crops and by-products is a renewable energy carrier that can potentially contribute to climate change mitigation. In this context, application of the methodology defined by the Renewable Energy Directive 2009/28/EC (RED) was performed in order to estimate the 100-year Global Warming Potential (GWP100) associated with an agro-biogas supply chain (SC) in Southern Italy. Doing so enabled calculation of Greenhouse Gas (GHG) emission saving in order to verify if it is at least equal to 35% compared to the fossil fuel reference system, as specified by the RED. For the assessment, an attributional Life Cycle Assessment (LCA) approach (International Organization for Standardization (ISO), 2006a,b) was integrated with the RED methodology applied following the guidelines reported in COM(2010)11 and updated by SWD(2014)259 and Report EUR 27215 EN (2015). Moreover, primary data were collected with secondary data extrapolated from the Ecoinvent database system. Results showed that the GWP100 associated with electricity production through the biogas plant investigated was equal to 111.58gCO2eqMJe(-1) and so a 40.01% GHG-emission saving was recorded compared to the RED reference. The highest contribution comes from biomass production and, in particular, from crop cultivation due to production of ammonium nitrate in the overall amount used for crop cultivation. Based upon the findings of the study, the GHG saving calculated slightly exceeds the related minimum proposed by the RED: therefore, improvements are needed anyway. In particular, the authors documented that through replacement of ammonium nitrate with urea the GHG-emission saving would increase to almost 68%, thus largely satisfying the RED limit. In addition, the study highlighted that conservation practices, such as NT, can significantly enable reduction of the GHG-emissions coming from agricultural activities. Therefore, those practices should be increasingly adopted for cultivation of energy

  5. Evaluation of anaerobic degradation, biogas and digestate production of cereal silages using nylon-bags.

    PubMed

    Negri, Marco; Bacenetti, Jacopo; Fiala, Marco; Bocchi, Stefano

    2016-06-01

    In this study, the degradation efficiency and the biogas and digestate production during anaerobic digestion were evaluated for the cereal silages most used to feed biogas plants. To this purpose, silages of: maize from the whole plant, maize from the ear, triticale and wheat were digested, inside of nylon bags, in laboratory scale digesters, for 75days. Overall, the test involved 288 nylon bags. After 75days of digestion, the maize ear silage shows the highest degradation efficiency (about 98%) while wheat silage the lowest (about 83%). The biogas production ranges from 438 to 852Nm(3)/t of dry matter for wheat and ear maize silage, respectively. For all the cereal silages, the degradation as well as the biogas production are faster at the beginning of the digestion time. Digestate mass, expressed as percentage of the fresh matter, ranges from 38% to 84% for wheat and maize ear silage, respectively. PMID:26946439

  6. BIOGAS Process development

    SciTech Connect

    Ghosh, S.; Mensinger, M.C.; Sajjad, A.; Henry, M.P.

    1984-01-01

    The overall objective of the program is to demonstrate and commercialize the IGT two-phase BIOGAS Process for optimized methane production from, and simultaneous stabilization of, municipal solid waste (MSW). The specific objective of the current program is to conduct a laboratory-scale investigation of simple, cost-effective feed pretreatment techniques and selected digestion reactor designs to optimize methane production from MSW-sludge blends, and to select the best pretreatment and digestion conditions for testing during the subsequent program for process development unit (PDU) operation. A significant portion of the program efforts to date has been directed at evaluating and/or developing feeding, mixing and discharging systems for handling high concentration, large particle size RDF slurries for anaerobic digestion processes. The performance of such processes depends significantly on the operational success of these subsystems. The results of the subsystem testing have been implemented in the design and operation of the 10-L, 20-L, and 125-L digesters. These results will also be utilized to design the CSTR and the upflow digesters of a large two-phase system. Data collected during the initial phase of this research showed in general that methane production from RDF decreased as the loading rate was increased. Thermophilic digestion did not appear to be significantly better than mesophlic digestion. 9 figures, 3 tables.

  7. The mechanisms of plant stress mitigation by kaolin-based particle films and its applications in horticultural and agricultural crops

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Kaolin-based particle films have utility in reducing insect, heat, light, and uv stress in plants due to the reflective nature of the particles. Particle films with a residue density of 1 to 3 g/ square meter have been evaluated in a range of crops and agricultural environments. The particle film ...

  8. Interactive Agricultural Ecological Atlas of Russia and Neighboring Countries:Economic Plants and their Diseases, Pests and Weeds.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The AgroAtlas is a comprehensive on-line bilingual reference on the geographic distribution of economic plants, their diseases, pests and weeds, and environmental factors that influence agricultural production through out the Former Soviet Union. Online users can read about and examine maps and ima...

  9. Seasonal OVOC fluxes from an agricultural field planted with sugar beet

    NASA Astrophysics Data System (ADS)

    Custer, T. G.; Schade, G. W.

    2005-12-01

    Although agricultural crops are generally not strong isoprenoid emitters, they do emit a variety of other atmospherically significant species collectively known as oxygenated VOCs (OVOCs), such as methanol, acetaldehyde, or various hexenal and hexenol compounds. Many OVOCs have longer atmospheric lifetimes than isoprenoid compounds and can affect the atmosphere's oxidative potential at higher elevations and far from sources. We performed selected OVOC flux measurements for select species above an agricultural field planted with sugar beets ( B. vulgaris) in northern Germany in 2004 to better understand the magnitude and controls over these OVOC emissions. Virtual disjunct eddy covariance was used to measure fluxes beginning immediately following seeding and continuing until past harvest. A commercial PTR-MS provided mixing ratios of methanol (m/z 33), acetaldehyde (m/z 45), acetone (m/z 59), and the sum of the isoprene oxidation products methacrolein and methyl vinyl ketone (m/z 71) while 3D wind velocities were measured using a Gill R3 sonic anemometer. Here, we compare the fluxes of methanol and acetone over the growth cycle of sugar beet to plant development as measured by the leaf area index. Methanol fluxes ranged from approximately -0.05 to 0.15 mg C m-2 h-1 (mixing ratios from ~1 to 15 ppbv) and showed a clear diurnal cycle after the sugar beets established a significant leaf area. Acetone fluxes ranged from approximately -0.2 to 0.2 mg C m-2 h-1 (mixing ratios from ~0.2 to 3 ppb). Higher specific emissions were found during earlier growth stages. Methanol flux correlated strongly with latent heat flux (or alternatively, with canopy conductance derived from the latent heat flux), while acetone flux did not. Acetone flux was small compared to methanol flux and sugar beet is likely not a significant acetone emitter. Weekly measurements of soil OVOC exchange using a flux chamber showed that the soil may have contributed significantly to the overall flux values

  10. Biodiversity and Ecosystem Functioning: Exploring Principles of Ecology with Agricultural Plants

    ERIC Educational Resources Information Center

    Ruesink, Jennifer; O'Connor, Eileen; Sparks, Grace

    2006-01-01

    To date, little of the ecological research on biological diversity and ecosystem functioning has been carried out in agricultural systems, despite the fact that agriculture is a major contributor to loss of native habitats and species. However, agricultural research has demonstrated that polycultures of multiple crop species can have higher total…

  11. Plant Growth Promoting Rhizobacteria and Mycorrhizal Fungi in Sustainable Agriculture and Forestry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plant-growth promoting rhizobacteria (PGPR) encourage plant growth by producing growth regulators, facilitating nutrient uptake, accelerating mineralization, reducing plant stress, stimulating nodulation, providing nitrogen fixation, promoting mycorrhizal fungi, suppressing plant diseases, and funct...

  12. Improvement of biogas production by bioaugmentation.

    PubMed

    Kovács, K L; Ács, N; Kovács, E; Wirth, R; Rákhely, G; Strang, Orsolya; Herbel, Zsófia; Bagi, Z

    2013-01-01

    Biogas production technologies commonly involve the use of natural anaerobic consortia of microbes. The objective of this study was to elucidate the importance of hydrogen in this complex microbial food chain. Novel laboratory biogas reactor prototypes were designed and constructed. The fates of pure hydrogen-producing cultures of Caldicellulosiruptor saccharolyticus and Enterobacter cloacae were followed in time in thermophilic and mesophilic natural biogas-producing communities, respectively. Molecular biological techniques were applied to study the altered ecosystems. A systematic study in 5-litre CSTR digesters revealed that a key fermentation parameter in the maintenance of an altered population balance is the loading rate of total organic solids. Intensification of the biogas production was observed and the results corroborate that the enhanced biogas productivity is associated with the increased abundance of the hydrogen producers. Fermentation parameters did not indicate signs of failure in the biogas production process. Rational construction of more efficient and sustainable biogas-producing microbial consortia is proposed. PMID:23484123

  13. Improvement of Biogas Production by Bioaugmentation

    PubMed Central

    Kovács, K. L.; Ács, N.; Kovács, E.; Wirth, R.; Rákhely, G.; Strang, Orsolya; Herbel, Zsófia; Bagi, Z.

    2013-01-01

    Biogas production technologies commonly involve the use of natural anaerobic consortia of microbes. The objective of this study was to elucidate the importance of hydrogen in this complex microbial food chain. Novel laboratory biogas reactor prototypes were designed and constructed. The fates of pure hydrogen-producing cultures of Caldicellulosiruptor saccharolyticus and Enterobacter cloacae were followed in time in thermophilic and mesophilic natural biogas-producing communities, respectively. Molecular biological techniques were applied to study the altered ecosystems. A systematic study in 5-litre CSTR digesters revealed that a key fermentation parameter in the maintenance of an altered population balance is the loading rate of total organic solids. Intensification of the biogas production was observed and the results corroborate that the enhanced biogas productivity is associated with the increased abundance of the hydrogen producers. Fermentation parameters did not indicate signs of failure in the biogas production process. Rational construction of more efficient and sustainable biogas-producing microbial consortia is proposed. PMID:23484123

  14. Research Progress on the use of Plant Allelopathy in Agriculture and the Physiological and Ecological Mechanisms of Allelopathy.

    PubMed

    Cheng, Fang; Cheng, Zhihui

    2015-01-01

    Allelopathy is a common biological phenomenon by which one organism produces biochemicals that influence the growth, survival, development, and reproduction of other organisms. These biochemicals are known as allelochemicals and have beneficial or detrimental effects on target organisms. Plant allelopathy is one of the modes of interaction between receptor and donor plants and may exert either positive effects (e.g., for agricultural management, such as weed control, crop protection, or crop re-establishment) or negative effects (e.g., autotoxicity, soil sickness, or biological invasion). To ensure sustainable agricultural development, it is important to exploit cultivation systems that take advantage of the stimulatory/inhibitory influence of allelopathic plants to regulate plant growth and development and to avoid allelopathic autotoxicity. Allelochemicals can potentially be used as growth regulators, herbicides, insecticides, and antimicrobial crop protection products. Here, we reviewed the plant allelopathy management practices applied in agriculture and the underlying allelopathic mechanisms described in the literature. The major points addressed are as follows: (1) Description of management practices related to allelopathy and allelochemicals in agriculture. (2) Discussion of the progress regarding the mode of action of allelochemicals and the physiological mechanisms of allelopathy, consisting of the influence on cell micro- and ultra-structure, cell division and elongation, membrane permeability, oxidative and antioxidant systems, growth regulation systems, respiration, enzyme synthesis and metabolism, photosynthesis, mineral ion uptake, protein and nucleic acid synthesis. (3) Evaluation of the effect of ecological mechanisms exerted by allelopathy on microorganisms and the ecological environment. (4) Discussion of existing problems and proposal for future research directions in this field to provide a useful reference for future studies on plant

  15. Research Progress on the use of Plant Allelopathy in Agriculture and the Physiological and Ecological Mechanisms of Allelopathy

    PubMed Central

    Cheng, Fang; Cheng, Zhihui

    2015-01-01

    Allelopathy is a common biological phenomenon by which one organism produces biochemicals that influence the growth, survival, development, and reproduction of other organisms. These biochemicals are known as allelochemicals and have beneficial or detrimental effects on target organisms. Plant allelopathy is one of the modes of interaction between receptor and donor plants and may exert either positive effects (e.g., for agricultural management, such as weed control, crop protection, or crop re-establishment) or negative effects (e.g., autotoxicity, soil sickness, or biological invasion). To ensure sustainable agricultural development, it is important to exploit cultivation systems that take advantage of the stimulatory/inhibitory influence of allelopathic plants to regulate plant growth and development and to avoid allelopathic autotoxicity. Allelochemicals can potentially be used as growth regulators, herbicides, insecticides, and antimicrobial crop protection products. Here, we reviewed the plant allelopathy management practices applied in agriculture and the underlying allelopathic mechanisms described in the literature. The major points addressed are as follows: (1) Description of management practices related to allelopathy and allelochemicals in agriculture. (2) Discussion of the progress regarding the mode of action of allelochemicals and the physiological mechanisms of allelopathy, consisting of the influence on cell micro- and ultra-structure, cell division and elongation, membrane permeability, oxidative and antioxidant systems, growth regulation systems, respiration, enzyme synthesis and metabolism, photosynthesis, mineral ion uptake, protein and nucleic acid synthesis. (3) Evaluation of the effect of ecological mechanisms exerted by allelopathy on microorganisms and the ecological environment. (4) Discussion of existing problems and proposal for future research directions in this field to provide a useful reference for future studies on plant

  16. Ecology of plant and free-living nematodes in natural and agricultural soil.

    PubMed

    Neher, Deborah A

    2010-01-01

    Nematodes are aquatic organisms that depend on thin water films to live and move within existing pathways of soil pores of 25-100 mum diameter. Soil nematodes can be a tool for testing ecological hypotheses and understanding biological mechanisms in soil because of their central role in the soil food web and linkage to ecological processes. Ecological succession is one of the most tested community ecology concepts, and a variety of nematode community indices have been proposed for purposes of environmental monitoring. In contrast, theories of biogeography, colonization, optimal foraging, and niche partitioning by nematodes are poorly understood. Ecological hypotheses related to strategies of coexistence of nematode species sharing the same resource have potential uses for more effective biological control and use of organic amendments to foster disease suppression. Essential research is needed on nematodes in natural and agricultural soils to synchronize nutrient release and availability relative to plant needs, to test ecological hypotheses, to apply optimal foraging and niche partitioning strategies for more effective biological control, to blend organic amendments to foster disease suppression, to monitor environmental and restoration status, and to develop better predictive models for land-use decisions. PMID:20455699

  17. Plant diversity and overyielding: insights from belowground facilitation of intercropping in agriculture.

    PubMed

    Li, Long; Tilman, David; Lambers, Hans; Zhang, Fu-Suo

    2014-07-01

    Despite increasing evidence that plant diversity in experimental systems may enhance ecosystem productivity, the mechanisms causing this overyielding remain debated. Here, we review studies of overyielding observed in agricultural intercropping systems, and show that a potentially important mechanism underlying such facilitation is the ability of some crop species to chemically mobilize otherwise-unavailable forms of one or more limiting soil nutrients such as phosphorus (P) and micronutrients (iron (Fe), zinc (Zn) and manganese (Mn)). Phosphorus-mobilizing crop species improve P nutrition for themselves and neighboring non-P-mobilizing species by releasing acid phosphatases, protons and/or carboxylates into the rhizosphere which increases the concentration of soluble inorganic P in soil. Similarly, on calcareous soils with a very low availability of Fe and Zn, Fe- and Zn-mobilizing species, such as graminaceous monocotyledonous and cluster-rooted species, benefit themselves, and also reduce Fe or Zn deficiency in neighboring species, by releasing chelating substances. Based on this review, we hypothesize that mobilization-based facilitative interactions may be an unsuspected, but potentially important mechanism enhancing productivity in both natural ecosystems and biodiversity experiments. We discuss cases in which nutrient mobilization might be occurring in natural ecosystems, and suggest that the nutrient mobilization hypothesis merits formal testing in natural ecosystems. PMID:25013876

  18. Agricultural Activities of a Meadow Eliminated Plant Litter from the Periphery of a Farmland in Inner Mongolia, China

    PubMed Central

    Kawada, Kiyokazu; Borjigin, Wuyunna; Nakamura, Toru

    2015-01-01

    The purpose of our investigation was to clarify the effects of agriculture on the process of loss of litter at the periphery of a farmland. This study revealed the generation process of an ecologically unusual phenomenon that is observed around cropland in semi-arid regions. We hypothesized that the vegetation around a farmland cannot supply plant litter to the ground surface because the ecological structure has been changed by agricultural activities. The study was conducted at Xilingol steppe, Xilingol League, Inner Mongolia Autonomous Region, China. Four study lines were established from the edge of an arable field to the surrounding meadow and parallel to the wind direction during the strong wind season. Key measurement for each line was set at the border between the farmland and steppe. Four study sites were set at intervals along each line. Plant litter, soil particle size distribution, plant species composition, plant volume, and species diversity were investigated. Despite using the same mowing method at the meadows of all study sites, the litter at the only periphery of the farmland completely disappeared. Soil particle size distribution in steppe, which was adjacent to the farmland, was similar to that of the farmland. Plant community structure at the periphery of the farmland was different from that of the far side from the farmland. This implies that soil scattered from the farmland affected the species composition of the steppe. Consequently, the change in plant community structure induced litter loss because of mowing. We concluded that plant litter was lost near the farmland because of the combined effects of farming and mowing. The results support our hypothesis that the vegetation around a farmland cannot supply plant litter because the ecological structure has been changed by agricultural activities. PMID:26241943

  19. Introduction and domestication of woody plants for sustainable agriculture in desert areas

    NASA Astrophysics Data System (ADS)

    Shelef, Oren; Soloway, Elaine; Rachmilevitch, Shimon

    2014-05-01

    plantation in arid conditions. 5) Balanites aegyptiaca is potentially a good biomass crop and good feed for grazers as goats. We illuminated differences related to drought tolerance between two distinct ecotypes. Attempts to develope sustainable agriculture based on local species will save resources (water, fertilizers, insecticides and herbicides), keep endangered plant species and enhance vegetation reestablishment.

  20. Biogas project advances in California

    SciTech Connect

    Wittrup, L.

    1995-04-01

    The National Renewable Energy Laboratory (NREL) has given a `thumbs up` rating to the high solids anaerobic digester project which is designed to produce biogas. The Sacramento Municipal Utility District (SMUD), the local utility, is considering the use of biogas to run a fuel cell pilot project. The designs for the three digesters are state-of-the-art, with each containing a horizontal trough measuring 120 feet long, 32 feet wide and 22 feet tall. NREL was asked by the PIA to review the mixing method in the digesters and analyze the overall potential success of the operation. The design employs a redundant system for foam removal from the digester gas, and has provisions to remove moisture from the biogas. However, there are no systems specified to reduce hydrogen sulfide levels. Since hydrogen sulfide is known to be corrosive, it may be destructive to the ultimate use as biogas in fuel cells. A suggested remedy from NREL is to add redundant iron sponge systems to remove hydrogen sulfide gases. A redundant system would allow regenerating one while the other is in service. In general, the lab found the design offers low construction costs, relative ease of operation, and a reasonably high level of anticipated success in operation. Therefore, NREL recommends proceeding with the current digester design plans, once the modifications as indicated are made.

  1. Native wildflower plantings support wild bee abundance and diversity in agricultural landscapes across the United States.

    PubMed

    Williams, Neal M; Ward, Kimiora L; Pope, Nathaniel; Isaacs, Rufus; Wilson, Julianna; May, Emily A; Ellis, Jamie; Daniels, Jaret; Pence, Akers; Ullmann, Katharina; Peters, Jeff

    2015-12-01

    Global trends in pollinator-dependent crops have raised awareness of the need to support managed and wild bee populations to ensure sustainable crop production. Provision of sufficient forage resources is a key element for promoting bee populations within human impacted landscapes, particularly those in agricultural lands where demand for pollination service is high and land use and management practices have reduced available flowering resources. Recent government incentives in North America and Europe support the planting of wildflowers to benefit pollinators; surprisingly, in North America there has been almost no rigorous testing of the performance of wildflower mixes, or their ability to support wild bee abundance and diversity. We tested different wildflower mixes in a spatially replicated, multiyear study in three regions of North America where production of pollinator-dependent crops is high: Florida, Michigan, and California. In each region, we quantified flowering among wildflower mixes composed of annual and perennial species, and with high and low relative diversity. We measured the abundance and species richness of wild bees, honey bees, and syrphid flies at each mix over two seasons. In each region, some but not all wildflower mixes provided significantly greater floral display area than unmanaged weedy control plots. Mixes also attracted greater abundance and richness of wild bees, although the identity of best mixes varied among regions. By partitioning floral display size from mix identity we show the importance of display size for attracting abundant and diverse wild bees. Season-long monitoring also revealed that designing mixes to provide continuous bloom throughout the growing season is critical to supporting the greatest pollinator species richness. Contrary to expectation, perennials bloomed in their first season, and complementarity in attraction of pollinators among annuals and perennials suggests that inclusion of functionally diverse

  2. Presence of plant protection products in three agricultural areas of Regione Lazio.

    PubMed

    Conte, E; Rossi, E; Spera, G; Pompi, V; Carfi', F; Spadoni, A R; Rosati, M; Montereali, M R; Donnarumma, L; Perconti, W

    2003-01-01

    Aim of the research was to verify the impact of plant protection products on three significant agricultural areas of Regione Lazio: Maccarese, Cisterna di Latina, Sabaudia-Terracina. This research studied the presence of some active ingredients, indicated by technicians as distributed, on soil, water, crop and air samples, the last one in greenhouse; the analysis, carried out by multi-residue methods, allowed to investigate also on a large amount of active ingredients not indicated by technicians. The determinations have been obtained, using internal standards, by GC-NPD, GC-ECD, HPLC-UV, HPLC-DAD, with different columns, conditions and wavelength of adsorption. Taking into account the results we could to assert that only a small part of the molecules searched were found in the samples and that they are typical for the crops and the environment treated. In greenhouse, more persistence was founded in wood greenhouse, treated from the outside, probably for the release of a.i. in time from wood and for a better distribution. The active ingredients more frequently founded must be controlled to avoid possible accumulation or leaching, especially for herbicides in the areas of Maccarese and Cisterna di Latina. Furthermore, the molecule on which more attention must be done when applied, are: linuron on carrots, penconazole on zucchini and cymoxanil, often used inappropriately, particularly on minor crops, like red-radish. Although the number of sampling was limited, it has been possible to outline the situation in the three zones considered, for directing choices, that could be more sensible at sanitary spin-off and at the environment. PMID:15151325

  3. Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity

    PubMed Central

    2014-01-01

    Current soil management strategies are mainly dependent on inorganic chemical-based fertilizers, which caused a serious threat to human health and environment. The exploitation of beneficial microbes as a biofertilizer has become paramount importance in agriculture sector for their potential role in food safety and sustainable crop production. The eco-friendly approaches inspire a wide range of application of plant growth promoting rhizobacteria (PGPRs), endo- and ectomycorrhizal fungi, cyanobacteria and many other useful microscopic organisms led to improved nutrient uptake, plant growth and plant tolerance to abiotic and biotic stress. The present review highlighted biofertilizers mediated crops functional traits such as plant growth and productivity, nutrient profile, plant defense and protection with special emphasis to its function to trigger various growth- and defense-related genes in signaling network of cellular pathways to cause cellular response and thereby crop improvement. The knowledge gained from the literature appraised herein will help us to understand the physiological bases of biofertlizers towards sustainable agriculture in reducing problems associated with the use of chemicals fertilizers. PMID:24885352

  4. Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity.

    PubMed

    Bhardwaj, Deepak; Ansari, Mohammad Wahid; Sahoo, Ranjan Kumar; Tuteja, Narendra

    2014-01-01

    Current soil management strategies are mainly dependent on inorganic chemical-based fertilizers, which caused a serious threat to human health and environment. The exploitation of beneficial microbes as a biofertilizer has become paramount importance in agriculture sector for their potential role in food safety and sustainable crop production. The eco-friendly approaches inspire a wide range of application of plant growth promoting rhizobacteria (PGPRs), endo- and ectomycorrhizal fungi, cyanobacteria and many other useful microscopic organisms led to improved nutrient uptake, plant growth and plant tolerance to abiotic and biotic stress. The present review highlighted biofertilizers mediated crops functional traits such as plant growth and productivity, nutrient profile, plant defense and protection with special emphasis to its function to trigger various growth- and defense-related genes in signaling network of cellular pathways to cause cellular response and thereby crop improvement. The knowledge gained from the literature appraised herein will help us to understand the physiological bases of biofertlizers towards sustainable agriculture in reducing problems associated with the use of chemicals fertilizers. PMID:24885352

  5. Indigenous and introduced arbuscular mycorrhizal fungi contribute to plant growth in two agricultural soils from south-western Australia.

    PubMed

    Gazey, C; Abbott, L K; Robson, A D

    2004-12-01

    Arbuscular mycorrhizal (AM) fungi occur in all agricultural soils but it is not easy to assess the contribution they make to plant growth under field conditions. Several approaches have been used to investigate this, including the comparison of plant growth in the presence or absence of naturally occurring AM fungi following soil fumigation or application of fungicides. However, treatments such as these may change soil characteristics other than factors directly involving AM fungi and lead to difficulties in identifying the reason for changes in plant growth. In a glasshouse experiment, we assessed the contribution of indigenous AM fungi to growth of subterranean clover in undisturbed cores of soil from two agricultural field sites (a cropped agricultural field at South Carrabin and a low input pasture at Westdale). We used the approach of estimating the benefit of AM fungi by comparing the curvature coefficients (C) of the Mitscherlich equation for subterranean clover grown in untreated field soil, in field soil into which inoculum of Glomus invermaium was added and in soil fumigated with methyl bromide. It was only possible to estimate the benefit of mycorrhizas using this approach for one soil (Westdale) because it was the only soil for which a Mitscherlich response to the application of a range of P levels was obtained. The mycorrhizal benefit (C of mycorrhizal vs. non-mycorrhizal plants or C of inoculated vs. uninoculated plants) of the indigenous fungi corresponded with a requirement for phosphate by plants that were colonised by AM fungi already present in the soil equivalent to half that required by non-mycorrhizal plants. This benefit was independent of the plant-available P in the soil. There was no additional benefit of inoculation on plant growth other than that due to increased P uptake. Indigenous AM fungi were present in both soils and colonised a high proportion of roots in both soils. There was a higher diversity of morphotypes of mycorrhizal fungi

  6. 75 FR 62096 - Agricultural Technical Advisory Committees for Trade in Tobacco, Cotton, Peanuts and Planting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-07

    ..., Peanuts and Planting Seeds, and Grains, Feed and Oilseeds; Re- structure and Realignment AGENCY: Foreign... Planting Seeds (TCPPS) and in Grains, Feed and Oilseeds (GFO). The Secretary is also soliciting comments as... representation of the planting seeds industry from the Tobacco, Cotton, Peanuts and Planting Seeds (TCPPS)...

  7. Plant available silicon in South-east Asian rice paddy soils - relevance of agricultural practice and of abiotic factors

    NASA Astrophysics Data System (ADS)

    Marxen, A.; Klotzbücher, T.; Vetterlein, D.; Jahn, R.

    2012-12-01

    Background Silicon (Si) plays a crucial role in rice production. Si content of rice plants exceeds the content of other major nutrients such as nitrogen, phosphorous or potassium. Recent studies showed that in some environments external supply of Si can enhance the growth of rice plants. Rice plants express specific Si transporters to absorb Si from soil solutions in form of silicic acid, which precipitates in tissue cells forming amorphous silica bodies, called phytoliths. The phytoliths are returned to soils with plant residues. They might be a main source of plant available silicic acid in soils. Aims In this study we assess the effects of rice paddy cultivation on the stocks of `reactive` Si fractions in mineral topsoils of rice paddy fields in contrasting landscapes. The `reactive` Si fractions are presumed to determine the release of plant-available silicic acid in soils. We consider the relevance of abiotic factors (mineral assemblage; soil weathering status) and agricultural practice for these fractions. Agricultural practices, which were assumed to affect the stocks of `reactive` Si were (i) the usage of different rice varieties (which might differ in Si demand), (ii) straw residue management (i.e., whether straw residues are returned to the fields or removed and used e.g. as fodder), and (iii) yield level and number of crops per year. Material and methods Soils (top horizon of about 0-20 cm depth) were sampled from rice paddy fields in 2 mountainous and 5 lowland landscapes of contrasting geologic conditions in Vietnam and the Philippines. Ten paddy fields were sampled per landscape. The rice paddy management within landscapes differed when different farmers and/or communities managed the fields. We analysed the following fractions of `reactive` Si in the soils: acetate-extractable Si (dissolved and easily exchangeable Si), phosphate-extractable Si (adsorbed Si), oxalate extractable Si (Si associated with poorly-ordered sesquioxides), NaOH extractable Si

  8. Educational Plant Survey. The University of Florida Institute of Food and Agricultural Sciences on Campus--Alachua County. April 4-5, 1994.

    ERIC Educational Resources Information Center

    Florida State Dept. of Education, Tallahassee.

    This report presents the results of a systematic study and evaluation of the existing educational plants of the University of Florida's Institute of Food and Agricultural Sciences (IFAS), along with a determination of future plant needs. Section 1 contains an introduction to the educational plant survey, including statutory foundations, procedural…

  9. Low-temperature upgrading of low-calorific biogas for CO2 mitigation using DBD-catalyst hybrid reactor

    NASA Astrophysics Data System (ADS)

    Nozaki, Tomohiro; Tsukijihara, Hiroyuki; Fukui, Wataru; Okazaki, Ken

    2006-10-01

    Although huge amounts of biogas, which consists of 20-60% of CH4 in CO2/N2, can be obtained from landfills, coal mines, and agricultural residues, most of them are simply flared and wasted: because global warming potential of biogas is 5-15 times as potent as CO2. Poor combustibility of such biogas makes it difficult to utilize in conventional energy system. The purpose of this project is to promote the profitable recovery of methane from poor biogas via non-thermal plasma technology. We propose low-temperature steam reforming of biogas using DBD generated in catalyst beds. Methane is partially converted into hydrogen, and then fed into internal combustion engines for improved ignition stability as well as efficient operation. Low-temperature steam reforming is beneficial because exhaust gas from an engine can be used to activate catalyst beds. Space velocity (3600-15000 hr-1), reaction temperature (300-650^oC), and energy cost (30-150 kJ per mol CH4) have been investigated with simulated biogas (20-60% CH4 in mixtures of CO2/N2). The DBD enhances reaction rate of CH4 by a factor of ten at given catalyst temperatures, which is a rate-determining step of methane steam reforming, while species concentration of upgraded biogas was governed by thermodynamic equilibrium in the presence of catalyst.

  10. Micro-aeration for hydrogen sulfide removal from biogas

    NASA Astrophysics Data System (ADS)

    Duangmanee, Thanapong

    was affected by changes in liquid heights the most, followed by changes in flow rates. Feasibility studies for H2S removal from biogas by micro-aeration were conducted at the Ames Water Pollution Control Facility (AWPCF) by using different types of liquid media available at the plant, i.e. plant effluent, mixed liquor, and digester supernatant. From the experiment at AWPCF, it was found that operating pHs were affected by the amount of alkalinity in the liquid media and that the removal efficiencies were affected by the operating pH. Among all the liquid media tested, digester supernatant showed the greatest potential with more than 99% H2S removal at an operating pH of 7.0 and volumetric biogas flow rate of 21.6 m3/m 3-hr. By increasing trace metal contents and temperature of the medium, the hydrogen sulfide removal rate was greatly improved. The operating cost of the full-scale system was estimated to be approximately $2/kg-S-removed. In addition, it was also revealed that abiotic sulfide oxidation accounted for 95% of overall sulfide oxidation. This technology is expected to widen the use of biogas as a renewable fuel since the maintenance requirements of biogas handling equipment, the methane purification costs, and the emissions of SOx will dramatically be reduced. Importantly, the technology does not require inoculation of special bacteria, addition of nutrients and trace elements, or chemicals for pH control.

  11. Employing native shrubs to improve agricultural potential of arid lands: Drawing on plants to draw water (Invited)

    NASA Astrophysics Data System (ADS)

    Dragila, M. I.; Kizito, F.; Dick, R.

    2009-12-01

    Even though soil moisture poses limits on landscape dynamics, plant communities within the landscape can also regulate the spatial distribution of moisture, thus creating a biofeedback system that advances the system towards a specific landscape order. This behavior is evident in arid climates where specific parameters, such as soil moisture, are close to sustainability limits and result in a distinct spatial distribution of plant communities. Understanding plant-soil water relationships can lead to management tools to improve landscape function. Plant-soil interactions that influence soil moisture include, local changes in soil texture when plants trap airborne soil particles, increases in organic matter content below their foliage, and root distribution. We specifically focus on a process commonly referred to as hydraulic redistribution wherein plant roots draw moisture vertically to the near surface, raising the potential for seed germination and maintenance through short drought periods. Two fieldwork sites in Senegal were used to investigate the role of native shrubs in controlling soil moisture movement, and in particular, using these native plants to enhance agricultural potential.

  12. Anaerobic Fungi and Their Potential for Biogas Production.

    PubMed

    Dollhofer, Veronika; Podmirseg, Sabine Marie; Callaghan, Tony Martin; Griffith, Gareth Wyn; Fliegerová, Kateřina

    2015-01-01

    Plant biomass is the largest reservoir of environmentally friendly renewable energy on earth. However, the complex and recalcitrant structure of these lignocellulose-rich substrates is a severe limitation for biogas production. Microbial pro-ventricular anaerobic digestion of ruminants can serve as a model for improvement of converting lignocellulosic biomass into energy. Anaerobic fungi are key players in the digestive system of various animals, they produce a plethora of plant carbohydrate hydrolysing enzymes. Combined with the invasive growth of their rhizoid system their contribution to cell wall polysaccharide decomposition may greatly exceed that of bacteria. The cellulolytic arsenal of anaerobic fungi consists of both secreted enzymes, as well as extracellular multi-enzyme complexes called cellulosomes. These complexes are extremely active, can degrade both amorphous and crystalline cellulose and are probably the main reason of cellulolytic efficiency of anaerobic fungi. The synergistic use of mechanical and enzymatic degradation makes anaerobic fungi promising candidates to improve biogas production from recalcitrant biomass. This chapter presents an overview about their biology and their potential for implementation in the biogas process. PMID:26337843

  13. Bioavailability of silver and silver sulfide nanoparticles to lettuce (Lactuca sativa): Effect of agricultural amendments on plant uptake.

    PubMed

    Doolette, Casey L; McLaughlin, Michael J; Kirby, Jason K; Navarro, Divina A

    2015-12-30

    Silver nanoparticles (AgNPs) can enter terrestrial systems as sulfidised AgNPs (Ag2S-NPs) through the application of biosolids to soil. However, the bioavailability of Ag2S-NPs in soils is unknown. The two aims of this study were to investigate (1) the bioavailability of Ag to lettuce (Lactuca sativa) using a soil amended with biosolids containing Ag2S-NPs and (2) the effect of commonly used agricultural fertilisers/amendments on the bioavailability of Ag, AgNPs and Ag2S-NPs to lettuce. The study used realistic AgNP exposure pathways and exposure concentrations. The plant uptake of Ag from biosolids-amended soil containing Ag2S-NPs was very low for all Ag treatments (0.02%). Ammonium thiosulfate and potassium chloride fertilisation significantly increased the Ag concentrations of plant roots and shoots. The extent of the effect varied depending on the type of Ag. Ag2S-NPs, the realistic form of AgNPs in soil, had the lowest bioavailability. The potential risk of AgNPs in soils is low; even in the plants that had the highest Ag concentrations (Ag(+)+thiosulfate), only 0.06% of added Ag was found in edible plant parts (shoots). Results from the study suggest that agricultural practises must be considered when carrying out risk assessments of AgNPs in terrestrial systems; such practises can affect AgNP bioavailability. PMID:26322966

  14. Comparisons of diazotrophic communities in native and agricultural desert ecosystems reveal plants as important drivers in diversity.

    PubMed

    Köberl, Martina; Erlacher, Armin; Ramadan, Elshahat M; El-Arabi, Tarek F; Müller, Henry; Bragina, Anastasia; Berg, Gabriele

    2016-02-01

    Diazotrophs provide the only biological source of fixed atmospheric nitrogen in the biosphere. Although they are the key player for plant-available nitrogen, less is known about their diversity and potential importance in arid ecosystems. We investigated the nitrogenase gene diversity in native and agricultural desert soil as well as within root-associated microbiota of medicinal plants grown in Egypt through the combination of nifH-specific qPCR, fingerprints, amplicon pyrosequencing and fluorescence in situ hybridization-confocal laser scanning microscopy. Although the diazotrophic microbiota were characterized by generally high abundances and diversity, statistically significant differences were found between both soils, the different microhabitats, and between the investigated plants (Matricaria chamomilla L., Calendula officinalis L. and Solanum distichum Schumach. and Thonn.). We observed a considerable community shift from desert to agriculturally used soil that demonstrated a higher abundance and diversity in the agro-ecosystem. The endorhiza was characterized by lower abundances and only a subset of species when compared to the rhizosphere. While the microbiomes of the Asteraceae were similar and dominated by potential root-nodulating rhizobia acquired primarily from soil, the perennial S. distichum generally formed associations with free-living nitrogen fixers. These results underline the importance of diazotrophs in desert ecosystems and additionally identify plants as important drivers in functional gene pool diversity. PMID:26705571

  15. Comparisons of diazotrophic communities in native and agricultural desert ecosystems reveal plants as important drivers in diversity

    PubMed Central

    Köberl, Martina; Erlacher, Armin; Ramadan, Elshahat M.; El-Arabi, Tarek F.; Müller, Henry; Bragina, Anastasia; Berg, Gabriele

    2015-01-01

    Diazotrophs provide the only biological source of fixed atmospheric nitrogen in the biosphere. Although they are the key player for plant-available nitrogen, less is known about their diversity and potential importance in arid ecosystems. We investigated the nitrogenase gene diversity in native and agricultural desert soil as well as within root-associated microbiota of medicinal plants grown in Egypt through the combination of nifH-specific qPCR, fingerprints, amplicon pyrosequencing and fluorescence in situ hybridization–confocal laser scanning microscopy. Although the diazotrophic microbiota were characterized by generally high abundances and diversity, statistically significant differences were found between both soils, the different microhabitats, and between the investigated plants (Matricaria chamomilla L., Calendula officinalis L. and Solanum distichum Schumach. and Thonn.). We observed a considerable community shift from desert to agriculturally used soil that demonstrated a higher abundance and diversity in the agro-ecosystem. The endorhiza was characterized by lower abundances and only a subset of species when compared to the rhizosphere. While the microbiomes of the Asteraceae were similar and dominated by potential root-nodulating rhizobia acquired primarily from soil, the perennial S. distichum generally formed associations with free-living nitrogen fixers. These results underline the importance of diazotrophs in desert ecosystems and additionally identify plants as important drivers in functional gene pool diversity. PMID:26705571

  16. Syntrophic acetate oxidation in industrial CSTR biogas digesters.

    PubMed

    Sun, Li; Müller, Bettina; Westerholm, Maria; Schnürer, Anna

    2014-02-10

    The extent of syntrophic acetate oxidation (SAO) and the levels of known SAO bacteria and acetate- and hydrogen-consuming methanogens were determined in sludge from 13 commercial biogas production plants. Results from these measurements were statistically related to the prevailing operating conditions, through partial least squares (PLS) analysis. This revealed that high abundance of microorganisms involved in SAO was positively correlated with relatively low abundance of aceticlastic methanogens and high concentrations of free ammonia (>160 mg/L) and volatile fatty acids (VFA). Temperature was identified as another influencing factor for the population structure of the syntrophic acetate oxidising bacteria (SAOB). Overall, there was a high abundance of SAOB in the different digesters despite differences in their operating parameters, indicating that SAOB are an enduring and important component of biogas-producing consortia. PMID:24333792

  17. Cow power: the energy and emissions benefits of converting manure to biogas

    NASA Astrophysics Data System (ADS)

    Cuéllar, Amanda D.; Webber, Michael E.

    2008-07-01

    This report consists of a top-level aggregate analysis of the total potential for converting livestock manure into a domestic renewable fuel source (biogas) that could be used to help states meet renewable portfolio standard requirements and reduce greenhouse gas (GHG) emissions. In the US, livestock agriculture produces over one billion tons of manure annually on a renewable basis. Most of this manure is disposed of in lagoons or stored outdoors to decompose. Such disposal methods emit methane and nitrous oxide, two important GHGs with 21 and 310 times the global warming potential of carbon dioxide, respectively. In total, GHG emissions from the agricultural sector in the US amounted to 536 million metric tons (MMT) of carbon dioxide equivalent, or 7% of the total US emissions in 2005. Of this agricultural contribution, 51 to 118 MMT of carbon dioxide equivalent resulted from livestock manure emissions alone, with trends showing this contribution increasing from 1990 to 2005. Thus, limiting GHG emissions from manure represents a valuable starting point for mitigating agricultural contributions to global climate change. Anaerobic digestion, a process that converts manure to methane-rich biogas, can lower GHG emissions from manure significantly. Using biogas as a substitute for other fossil fuels, such as coal for electricity generation, replaces two GHG sources—manure and coal combustion—with a less carbon-intensive source, namely biogas combustion. The biogas energy potential was calculated using values for the amount of biogas energy that can be produced per animal unit (defined as 1000 pounds of animal) per day and the number of animal units in the US. The 95 million animal units in the country could produce nearly 1 quad of renewable energy per year, amounting to approximately 1% of the US total energy consumption. Converting the biogas into electricity using standard microturbines could produce 88 ± 20 billion kWh, or 2.4 ± 0.6% of annual electricity

  18. Bioavailability and soil-to-plant transfer factors as indicators of potentially toxic element contamination in agricultural soils.

    PubMed

    Adamo, Paola; Iavazzo, Pietro; Albanese, Stefano; Agrelli, Diana; De Vivo, Benedetto; Lima, Annamaria

    2014-12-01

    Soil pollution in agricultural lands poses a serious threat to food safety, and suggests the need for consolidated methods providing advisory indications for soil management and crop production. In this work, the three-step extraction procedure developed by the EU Measurement and Testing Programme and two soil-to-plant transfer factors (relative to total and bioavailable concentration of elements in soil) were applied on polluted agricultural soils from southern Italy to obtain information on the retention mechanisms of metals in soils and on their level of translocation to edible vegetables. The study was carried out in the Sarno river plain of Campania, an area affected by severe environmental degradation potentially impacting the health of those consuming locally produced vegetables. Soil samples were collected in 36 locations along the two main rivers flowing into the plain. In 11 sites, lettuce plants were collected at the normal stage of consumption. According to Italian environmental law governing residential soils, and on the basis of soil background reference values for the study area, we found diffuse pollution by Be, Sn and Tl, of geogenic origin, Cr and Cu from anthropogenic sources such as tanneries and intensive agriculture, and more limited pollution by Pb, Zn and V. It was found that metals polluting soils as a result of human activities were mainly associated to residual, oxidizable and reducible phases, relatively immobile and only potentially bioavailable to plants. By contrast, the essential elements Zn and Cu showed a tendency to become more readily mobile and bioavailable as their total content in soil increased and were more easily transported to the edible parts of lettuce than other pollutants. According to our results, current soil pollution in the studied area does not affect the proportion of metals taken up by lettuce plants and there is a limited health risk incurred. PMID:25217740

  19. Gene flow in the green mirid, Creontiades dilutus (Hemiptera: Miridae), across arid and agricultural environments with different host plant species

    PubMed Central

    Hereward, J P; Walter, G H; DeBarro, P J; Lowe, A J; Riginos, C

    2013-01-01

    Creontiades dilutus (Stål), the green mirid, is a polyphagous herbivorous insect endemic to Australia. Although common in the arid interior of Australia and found on several native host plants that are spatially and temporally ephemeral, green mirids also reach pest levels on several crops in eastern Australia. These host-associated dynamics, distributed across a large geographic area, raise questions as to whether (1) seasonal fluctuations in population size result in genetic bottlenecks and drift, (2) arid and agricultural populations are genetically isolated, and (3) the use of different host plants results in genetic differentiation. We sequenced a mitochondrial COI fragment from individuals collected over 24 years and screened microsatellite variation from 32 populations across two seasons. The predominance of a single COI haplotype and negative Tajima D in samples from 2006/2007 fit with a population expansion model. In the older collections (1983 and 1993), a different haplotype is most prevalent, consistent with successive population contractions and expansions. Microsatellite data indicates recent migration between inland sites and coastal crops and admixture in several populations. Altogether, the data suggest that long-distance dispersal occurs between arid and agricultural regions, and this, together with fluctuations in population size, leads to temporally dynamic patterns of genetic differentiation. Host-associated differentiation is evident between mirids sampled from plants in the genus Cullen (Fabaceae), the primary host, and alternative host plant species growing nearby in arid regions. Our results highlight the importance of jointly assessing natural and agricultural environments in understanding the ecology of pest insects. PMID:23610626

  20. Impact of enzymatic pretreatment on corn stover degradation and biogas production.

    PubMed

    Schroyen, Michel; Vervaeren, Han; Van Hulle, Stijn W H; Raes, Katleen

    2014-12-01

    Corn stover is an agricultural residue consisting of lignocellulose, cellulose and hemicellulose polymers, sheeted in a lignin barrier. Corn stover can be used as feedstock for biogas production. Previous studies have shown biological pretreatment of lignocellulose materials can increase digestibility of the substrate improving hydrolysis, the rate-limiting step in biogas production. The impact of pretreating with different enzymes (laccase, manganese peroxidase and versatile peroxidase) and different incubation times, (0, 6 and 24 h) was studied. The effect on the matrix and biomethane production was determined. Pretreatments did not yield high concentrations of phenolic compounds, inhibitors of biogas production. The laccase enzyme showed an increase in biomethane production of 25% after 24 h of incubation. Pretreatment with peroxidase enzymes increased biomethane production with 17% after 6 h of incubation. As such it can be concluded that by introducing the different enzymes at different stages during pretreatment an increased biomethane production can be obtained. PMID:25285760

  1. Inhibition of proliferation by agricultural plant extracts in seven human adult T-cell leukaemia (ATL)-related cell lines.

    PubMed

    Kai, Hisahiro; Akamatsu, Ena; Torii, Eri; Kodama, Hiroko; Yukizaki, Chizuko; Sakakibara, Yoichi; Suiko, Masahito; Morishita, Kazuhiro; Kataoka, Hiroaki; Matsuno, Koji

    2011-07-01

    Adult T-cell leukaemia (ATL) is caused by human T-cell leukaemia virus type I (HTLV-I) infection and is resistant to conventional chemotherapy. We evaluated the inhibitory effects of agricultural plants on the proliferation of seven ATL-related human leukaemia cells, using three ATL cell lines (ED, Su9T01 and S1T), two human T-cell lines transformed by HTLV-I infection (HUT-102 and MT-2) and two HTLV-I-negative human T-cell acute lymphoblastic leukaemia cell lines (Jurkat and MOLT-4). A total of 52 samples of 80% ethanol extracts obtained from 30 types of agricultural plants were examined. On the basis of IC(50) values, we selected samples with greater activity than genistein, which was used as a positive control. The highest inhibitory effect was observed with extracts from leaves of Vaccinium virgatum Aiton (blueberry) on four cell lines (ED, Su9T01, HUT-102 and Jurkat); seeds of Momordica charantia L. (bitter gourd) exhibited the second highest activity. The bitter gourd seeds suppressed the proliferation of three cell lines (Su9T01, HUT-102 and Jurkat). The extracts from edible parts of Ipomea batatas LAM. (sweet potato), edible parts of Colocasia esculenta (L.) Schott (taro), skin of taro and seeds of Prunus mume Sieb. et Zucc. (mume) showed markedly greater inhibitory effects on Su9T01 than genistein. These findings suggest that ATL-preventative bioactive compounds may exist in these agricultural plants, which are considered to be functional foods. PMID:21293936

  2. THE USE OF CHEMICALS AS INSECTICIDES--PLANTS. AGRICULTURAL CHEMICALS TECHNOLOGY, NUMBER 2.

    ERIC Educational Resources Information Center

    Ohio State Univ., Columbus. Center for Vocational and Technical Education.

    THIS GUIDE IS ONE OF A SERIES DESIGNED TO PROVIDE GROUP INSTRUCTION AND INDIVIDUAL OCCUPATIONAL EXPERIENCE FOR POST-SECONDARY STUDENTS PREPARING FOR EMPLOYMENT AS AGRICULTURAL CHEMICAL TECHNICIANS. IT WAS DEVELOPED BY A NATIONAL TASK FORCE ON THE BASIS OF STATE STUDY DATA. THE OBJECTIVES ARE TO DEVELOP (1) INTEREST, APPRECIATION, AND UNDERSTANDING…

  3. Agriculture and Education, Planting the Seeds of Opportunity. USDA Adopt-a-School Program Guide.

    ERIC Educational Resources Information Center

    Department of Agriculture, Washington, DC. Office of the Secretary.

    This guide explains how U.S. Department of Agriculture (USDA) agencies can join the 20 other USDA agencies that have implemented 18 adopt-a-school partnerships throughout the country. The following are among the topics discussed in the guide's five parts: (1) partnership basics (how adopt-a-school programs benefit both members of the…

  4. Marshner Review: Harnessing the rhizosphere microbiome through plant breeding and agricultural management

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The need to enhance the sustainability of intensive agricultural systems is widely recognized. One promising approach is to encourage beneficial functions provided by microorganisms to decrease the inputs of fertilizers and pesticides. However, tremendous uncertainty exists in how this might be best...

  5. Infrared Spectroscopy as a Versatile Analytical Tool for the Quantitative Determination of Antioxidants in Agricultural Products, Foods and Plants

    PubMed Central

    Cozzolino, Daniel

    2015-01-01

    Spectroscopic methods provide with very useful qualitative and quantitative information about the biochemistry and chemistry of antioxidants. Near infrared (NIR) and mid infrared (MIR) spectroscopy are considered as powerful, fast, accurate and non-destructive analytical tools that can be considered as a replacement of traditional chemical analysis. In recent years, several reports can be found in the literature demonstrating the usefulness of these methods in the analysis of antioxidants in different organic matrices. This article reviews recent applications of infrared (NIR and MIR) spectroscopy in the analysis of antioxidant compounds in a wide range of samples such as agricultural products, foods and plants. PMID:26783838

  6. Biogas production from Jatropha curcas press-cake

    SciTech Connect

    Staubmann, R.; Guebitz, G.M.; Lafferty, R.M.

    1997-12-31

    Seeds of the tropical plant Jatropha curcas (purge nut, physic nut) are used for the production of oil. Several methods for oil extraction have been developed. In all processes, about 50% of the weight of the seeds remain as a press cake containing mainly protein and carbohydrates. Investigations have shown that this residue contains toxic compounds and cannot be used as animal feed without further processing. Preliminary experiments have shown that the residue is a good substrate for biogas production. Biogas formation was studied using a semicontinous upflow anaerobic sludge blanket (UASB) reactor; a contact-process and an anaerobic filter each reactor having a total volume of 110 L. A maximum production rate of 3.5 m{sup 3} m{sup -3} d{sup -1} was obtained in the anaerobic filter with a loading rate of 13 kg COD m{sup -3} d{sup -1}. However, the UAS reactor and the contact-process were not suitable for using this substrate. When using an anaerobic filter with Jatropha curcas seed cake as a substrate, 76% of the COD was degraded and 1 kg degraded COD yielded 355 L of biogas containing 70% methane. 28 refs., 3 figs., 4 tabs.

  7. Toward policies for climate change mitigation: "Barriers for family-sized biogas in the District of Gihanga, Burundi"

    NASA Astrophysics Data System (ADS)

    Nkunzimana, Leonard; Huart, Michel; Zaccai, Edwin

    2014-05-01

    In the context of climate change mitigation and poverty reduction, it has been argued that biogas energy is relevant, as it is economically and ecologically useful. In the 1980s, biogas use played an important role in the development of Burundi. Many schools and public institutions had implemented such installations. Unfortunately, many biogas infrastructures were destroyed in the civil war of the 1990s. This study analyzes what could be done, after a decade of crisis, to develop that sector. It aims to assess how and to what extent the inhabitants of villages are willing to contribute to the development of biogas technologies. We interviewed 150 farmers in order to assess their perception on the ecologic and economic features of biogas plants if implemented in their villages. The influence of socioeconomic, cultural, and demographic factors of households was assessed in this study. Results suggest that the maximum amount that a household is willing to pay each month for biogas use at a family level is positive for large-size households, households that are aware of climate change, consumers of candles, households with high income, households with an educated head, women, and breeders. However, the willingness decreases for households with older head of families. The study concludes that awareness campaigns on biogas benefits and financial and nonfinancial incentives are necessary. This policy should probably and primarily be oriented toward some more receptive categories of the population. Women should be fully involved, considering their positive motivation toward sustaining this sector.

  8. Liquid membrane purification of biogas

    SciTech Connect

    Majumdar, S.; Guha, A.K.; Lee, Y.T.; Papadopoulos, T.; Khare, S. . Dept. of Chemistry and Chemical Engineering)

    1991-03-01

    Conventional gas purification technologies are highly energy intensive. They are not suitable for economic removal of CO{sub 2} from methane obtained in biogas due to the small scale of gas production. Membrane separation techniques on the other hand are ideally suited for low gas production rate applications due to their modular nature. Although liquid membranes possess a high species permeability and selectivity, they have not been used for industrial applications due to the problems of membrane stability, membrane flooding and poor operational flexibility, etc. A new hollow-fiber-contained liquid membrane (HFCLM) technique has been developed recently. This technique overcomes the shortcomings of the traditional immobilized liquid membrane technology. A new technique uses two sets of hydrophobic, microporous hollow fine fibers, packed tightly in a permeator shell. The inter-fiber space is filled with an aqueous liquid acting as the membrane. The feed gas mixture is separated by selective permeation of a species through the liquid from one fiber set to the other. The second fiber set carries a sweep stream, gas or liquid, or simply the permeated gas stream. The objectives (which were met) of the present investigation were as follows. To study the selective removal of CO{sub 2} from a model biogas mixture containing 40% CO{sub 2} (the rest being N{sub 2} or CH{sub 4}) using a HFCLM permeator under various operating modes that include sweep gas, sweep liquid, vacuum and conventional permeation; to develop a mathematical model for each mode of operation; to build a large-scale purification loop and large-scale permeators for model biogas separation and to show stable performance over a period of one month.

  9. Assessment of heavy metal and pesticide levels in soil and plant products from agricultural area of Belgrade, Serbia.

    PubMed

    Marković, Mirjana; Cupać, Svjetlana; Durović, Rada; Milinović, Jelena; Kljajić, Petar

    2010-02-01

    This study was aimed to assess the levels of selected heavy metals and pesticides in soil and plant products from an agricultural area of Belgrade, Serbia and to indicate possible sources and risks of contamination. Soil, vegetable, and fruit samples from the most important agricultural city areas were collected from July to November of 2006. Metal contents were determined by atomic absorption spectrometry, whereas pesticide residues were analyzed by gas chromatography-mass spectrometry after extraction performed using solid-phase microextraction technique. Soil characterization based on the determination of selected physical and chemical properties revealed heterogeneous soils belonging to different soil groups. The concentrations of lead, cadmium, copper, and zinc in soil samples do not exceed the limits established by national and international regulations. Residues of the herbicide atrazine were detected in three soil samples, with levels lower than the relevant limit. The presence of other herbicides, namely prometryn, chloridazon, acetochlor, flurochloridone, and napropamide, was registered in some soil samples as well. Among the insecticides investigated in the soil, fenitrothion and chlorpyrifos were the only ones detected. In most of the investigated vegetable samples from the Obrenovac area, Pb and Cd contents are higher in comparison with the maximum levels, indicating the emission of coal combustion products from local thermal power plants as a possible source of contamination. Residue levels of some herbicides and insecticides (metribuzin, trifluralin, pendimethalin, bifenthrin, chlorpyrifos, and cypermethrin) determined in tomato, pepper, potato, and onion samples from Slanci, Ovca, and Obrenovac areas are even several times higher than the maximum residue levels. Inappropriate use of these plant protection products is considered to be the most probable reason of contamination. Because increased levels of heavy metals and pesticide residues found in

  10. Strategy for the Development of a Smart NDVI Camera System for Outdoor Plant Detection and Agricultural Embedded Systems

    PubMed Central

    Dworak, Volker; Selbeck, Joern; Dammer, Karl-Heinz; Hoffmann, Matthias; Zarezadeh, Ali Akbar; Bobda, Christophe

    2013-01-01

    The application of (smart) cameras for process control, mapping, and advanced imaging in agriculture has become an element of precision farming that facilitates the conservation of fertilizer, pesticides, and machine time. This technique additionally reduces the amount of energy required in terms of fuel. Although research activities have increased in this field, high camera prices reflect low adaptation to applications in all fields of agriculture. Smart, low-cost cameras adapted for agricultural applications can overcome this drawback. The normalized difference vegetation index (NDVI) for each image pixel is an applicable algorithm to discriminate plant information from the soil background enabled by a large difference in the reflectance between the near infrared (NIR) and the red channel optical frequency band. Two aligned charge coupled device (CCD) chips for the red and NIR channel are typically used, but they are expensive because of the precise optical alignment required. Therefore, much attention has been given to the development of alternative camera designs. In this study, the advantage of a smart one-chip camera design with NDVI image performance is demonstrated in terms of low cost and simplified design. The required assembly and pixel modifications are described, and new algorithms for establishing an enhanced NDVI image quality for data processing are discussed. PMID:23348037

  11. Strategy for the development of a smart NDVI camera system for outdoor plant detection and agricultural embedded systems.

    PubMed

    Dworak, Volker; Selbeck, Joern; Dammer, Karl-Heinz; Hoffmann, Matthias; Zarezadeh, Ali Akbar; Bobda, Christophe

    2013-01-01

    The application of (smart) cameras for process control, mapping, and advanced imaging in agriculture has become an element of precision farming that facilitates the conservation of fertilizer, pesticides, and machine time. This technique additionally reduces the amount of energy required in terms of fuel. Although research activities have increased in this field, high camera prices reflect low adaptation to applications in all fields of agriculture. Smart, low-cost cameras adapted for agricultural applications can overcome this drawback. The normalized difference vegetation index (NDVI) for each image pixel is an applicable algorithm to discriminate plant information from the soil background enabled by a large difference in the reflectance between the near infrared (NIR) and the red channel optical frequency band. Two aligned charge coupled device (CCD) chips for the red and NIR channel are typically used, but they are expensive because of the precise optical alignment required. Therefore, much attention has been given to the development of alternative camera designs. In this study, the advantage of a smart one-chip camera design with NDVI image performance is demonstrated in terms of low cost and simplified design. The required assembly and pixel modifications are described, and new algorithms for establishing an enhanced NDVI image quality for data processing are discussed. PMID:23348037

  12. Caesium-137 soil-to-plant transfer for representative agricultural crops of monocotyledonous and dicotyledonous plants in post-Chernobyl steppe landscape

    NASA Astrophysics Data System (ADS)

    Paramonova, Tatiana; Komissarova, Olga; Turykin, Leonid; Kuzmenkova, Natalia; Belyaev, Vladimir

    2016-04-01

    The accident at the Chernobyl nuclear power plant in 1986 had a large-scale action on more than 2.3 million hectares agricultural lands in Russia. The area of radioactively contaminated chernozems of semi-arid steppe zone with initial levels of Cs-137 185-555 kBq/m2 in Tula region received the name "Plavsky radioactive hotspot". Nowadays, after the first half-life period of Cs-137 arable chernozems of the region are still polluted with 3-6-fold excess above the radioactive safety standard (126-228 kBq/m2). Therefore, qualitative and quantitative characteristics of Cs-137 soil-to-plant transfer are currently a central problem for land use on the territory. The purpose of the present study was revealing the biological features of Cs-137 root uptake from contaminated arable chernozems by different agricultural crops. The components of a grass mixture growing at the central part of Plavsky radioactive hotspot with typical dicotyledonous and monocotyledonous plants - galega (Galega orientalis, Fabaceae family) and bromegrass (Bromus inermis, Gramineae family) respectively - were selected for the investigation, that was conducted during the period of harvesting in 2015. An important point was that the other factors influenced on Cs-137 soil-to-plant transfer - the level of soil pollution, soil properties, climatic conditions, vegetative phase, etc. - were equal. So, biological features of Cs-137 root uptake could be estimated the most credible manner. As a whole, general discrimination of Cs-137 root uptake was clearly shown for both agricultural crops. Whereas Cs-137 activity in rhizosphere 30-cm layer of arable chernozem was 371±74 Bq/kg (140±32 kBq/m2), Cs-137 activities in plant biomass were one-two orders of magnitude less, and transfer factor (TF) values (the ratio of the Cs-137 activities in vegetation and in soil) not exceeded 0.11. At the same time bioavailability of Cs-137 for bromegrass was significantly higher than for galega: TFs in total biomass of the

  13. The ozone component of global changes: Potential effects on agricultural and horticultural plant yield, product quality and interactions with invasive species

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The performance and quality of agricultural and horticultural plants in many regions of the world are adversely affected by current and anticipated concentrations of ground-level, tropospheric ozone (O3). Numerous studies with a variety of plant species and experimental approaches lead to similar ...

  14. A method of variable spacing for controlled plant growth systems in spaceflight and terrestrial agriculture applications

    NASA Technical Reports Server (NTRS)

    Knox, J.

    1986-01-01

    A higher plant growth system for Controlled Ecological Life Support System (CELSS) applications is described. The system permits independent movement of individual plants during growth. Enclosed within variable geometry growth chambers, the system allocates only the volume required by the growing plants. This variable spacing system maintains isolation between root and shoot environments, providing individual control for optimal growth. The advantages of the system for hydroponic and aeroponic growth chambers are discussed. Two applications are presented: (1) the growth of soybeans in a space station common module, and (2) in a terrestrial city greenhouse.

  15. Vulnerability of Rehabilitated Agricultural Production Systems to Invasion by Nontarget Plant Species

    NASA Astrophysics Data System (ADS)

    Baer, Sara G.; Engle, David M.; Knops, Johannes M. H.; Langeland, Kenneth A.; Maxwell, Bruce D.; Menalled, Fabian D.; Symstad, Amy J.

    2009-02-01

    Vast areas of arable land have been retired from crop production and “rehabilitated” to improved system states through landowner incentive programs in the United States (e.g., Conservation and Wetland Reserve Programs), as well as Europe (i.e., Agri-Environment Schemes). Our review of studies conducted on invasion of rehabilitated agricultural production systems by nontarget species elucidates several factors that may increase the vulnerability of these systems to invasion. These systems often exist in highly fragmented and agriculturally dominated landscapes, where propagule sources of target species for colonization may be limited, and are established under conditions where legacies of past disturbance persist and prevent target species from persisting. Furthermore, rehabilitation approaches often do not include or successfully attain all target species or historical ecological processes (e.g., hydrology, grazing, and/or fire cycles) key to resisting invasion. Uncertainty surrounds ways in which nontarget species may compromise long term goals of improving biodiversity and ecosystem services through rehabilitation efforts on former agricultural production lands. This review demonstrates that more studies are needed on the extent and ecological impacts of nontarget species as related to the goals of rehabilitation efforts to secure current and future environmental benefits arising from this widespread conservation practice.

  16. Evaluation of Shiraz wastewater treatment plant effluent quality for agricultural irrigation by Canadian Water Quality Index (CWQI)

    PubMed Central

    2013-01-01

    Background Using treated wastewater in agriculture irrigation could be a realistic solution for the shortage of fresh water in Iran, however, it is associated with environmental and health threats; therefore, effluent quality assessment is quite necessary before use. The present study aimed to evaluate the physicochemical and microbial quality of Shiraz wastewater treatment plant effluent for being used in agricultural irrigation. In this study, 20 physicochemical and 3 microbial parameters were measured during warm (April to September) and cold months (October to march). Using the measured parameters and the Canadian Water Quality Index, the quality of the effluent was determined in both warm and cold seasons and in all the seasons together. Results The calculated index for the physicochemical parameters in the effluent was equal (87) in warm and cold months and it was obtained as 85 for the seasons all together. When the microbial parameters were used in order to calculate the index, it declined to 67 in warm and cold seasons and 64 in all the seasons together. Also, it was found that three physicochemical parameters (TDS, EC, and NO3) and three microbial parameters (Fecal coliform, Helminthes egg, and Total coliform) had the most contribution to the reduction of the index value. Conclusions The results showed that the physicochemical quality of Shiraz Wastewater Treatment Plant Effluent was good for irrigation in the warm, cold, and total of the two kinds of seasons. However, by applying the microbial parameter, the index value declined dramatically and the quality of the effluent was marginal. PMID:23566673

  17. Effects of fluoride emissions on enzyme activity in metabolism of agricultural plants

    SciTech Connect

    Moeri, P.B.

    1980-01-01

    The effects of fluoride on the activity of malatedehydrogenase (MDH) in rape seed and rye grass have been investigated. Fluoride, which has been absorbed from the air, seems to act differently from fluoride added to the soil. The action of airborne fluoride compounds resorbed by the plant on the activity of MDH significantly correlated with the distance from an aluminum plant, crop yield, and fluoride content. 5 references, 5 figures, 2 tables.

  18. Fate of hazardous elements in agricultural soils surrounding a coal power plant complex from Santa Catarina (Brazil).

    PubMed

    Rodriguez-Iruretagoiena, Azibar; Fdez-Ortiz de Vallejuelo, Silvia; Gredilla, Ainara; Ramos, Claudete G; Oliveira, Marcos L S; Arana, Gorka; de Diego, Alberto; Madariaga, Juan Manuel; Silva, Luis F O

    2015-03-01

    Hazard element contamination coming from coal power plants is something obvious, but when this contamination is accompanied by other contamination sources, such as, urban, coal mining and farming activities the study gets complicated. This is the case of an area comprised in the southern part of Santa Catarina state (Brazil) with the largest private power plant generator. After the elemental analysis of 41 agricultural soils collected in an extensive area around the thermoelectric (from 0 to 47 km), the high presence of As, Co, Cr, Cu, Fe, Mn, Mo, Pb, Sb, Sn, Tl, V and Zn was found in some specific areas around the power plant. Nevertheless, as the NWAC (Normalized-and-Weighted Average Concentration) confirmed, only soils from one site were classified as of very high concern due to the presence of potential toxic elements. This site was located within the sedimentation basin of the power plant. The spatial distribution obtained by kriging in combination with the analysis of the data by Principal Component Analysis (PCA) revealed three important hotspots in the area according to soil uses and geographic localization: the thermoelectric, its area of influence due to volatile compound deposition, and the area comprised between two urban areas. Farming practice turn out to be an important factor too for the quantity of hazard element stored in soils. PMID:25497677

  19. Passive solar technology aids biogas digesters

    SciTech Connect

    Not Available

    1988-07-01

    Farming communities throughout China rely on biogas generators as a primary source of light and heat, as well as using the sludge as a nitrogen-rich fertilizer. Now researchers at Beijing's Solar Energy Laboratory have improved efficiency by building a rectangular tank out of concrete slabs, with one slanted surface painted black and covered with glass. According to a report in New Scientist, this passive solar panel generates heat in the same way as a greenhouse, raising inside temperatures by 10{degree}C and increasing biogas production by 50%. Another advantage of the new tanks is easy access, since the tank's lid sites in wells of water which form a seal against oxygen. (Old biogas tanks were made of soil, sand and a little concrete, prone to developing severe cracks which would allow oxygen to enter thus slowing down anaerobic reaction). Explains Debora MacKenzie of New Scientist: with the new tank, the farmer can simply remove the lid and attack the contents with a spade. This means that the mixture can comprise more than 10% solids. Greater density allows smaller tanks. Rural families need one cubic meter of biogas daily for light and heat; instead of the former 8 cm biogas generator, the new tanks need only be 1 cm. The prediction is that the smaller size could make biogas more popular in China's crowded towns. The biogas department is headed by He Shao Qi, who is also investigating ways to reduce production costs for the tanks.

  20. Herbivore-induced plant volatiles to enhance biological control in agriculture.

    PubMed

    Peñaflor, M F G V; Bento, J M S

    2013-08-01

    Plants under herbivore attack synthetize defensive organic compounds that directly or indirectly affect herbivore performance and mediate other interactions with the community. The so-called herbivore-induced plant volatiles (HIPVs) consist of odors released by attacked plants that serve as important cues for parasitoids and predators to locate their host/prey. The understanding that has been gained on the ecological role and mechanisms of HIPV emission opens up paths for developing novel strategies integrated with biological control programs with the aim of enhancing the efficacy of natural enemies in suppressing pest populations in crops. Tactics using synthetic HIPVs or chemically/genetically manipulating plant defenses have been suggested in order to recruit natural enemies to plantations or help guiding them to their host more quickly, working as a "synergistic" agent of biological control. This review discusses strategies using HIPVs to enhance biological control that have been proposed in the literature and were categorized here as: (a) exogenous application of elicitors on plants, (b) use of plant varieties that emit attractive HIPVs to natural enemies, (c) release of synthetic HIPVs, and (d) genetic manipulation targeting genes that optimize HIPV emission. We discuss the feasibility, benefits, and downsides of each strategy by considering not only field studies but also comprehensive laboratory assays that present an applied approach for HIPVs or show the potential of employing them in the field. PMID:23949852

  1. Biogas production from water hyacinth and channel grass used for phytoremediation of industrial effluents.

    PubMed

    Singhal, V; Rai, J P N

    2003-02-01

    The paper reports on the biogas production from water hyacinth (Eichhornia crassipes) and channel grass (Vallisneria spiralis) employed separately for phytoremediation of lignin and metal-rich pulp and paper mill and highly acidic distillery effluents. These plants eventually grow well in diluted effluent up to 40% (i.e., 2.5-times dilution with deionized water) and often take up metals and toxic materials from wastewater for their metabolic use. Slurry of the two plants used for phytoremediation produced significantly more biogas than that produced by the plants grown in deionized water; the effect being more marked with plants used for phytoremediation of 20% pulp and paper mill effluent. Biogas production from channel grass was relatively greater and quicker (maximum in 6-9 days) than that from water hyacinth (in 9-12 days). Such variation in biogas production by the two macrophytes has been correlated with the changes in C, N and C/N ratio of their slurry brought by phytoremediation. PMID:12688463

  2. Novel Sorbent to Clean Biogas for Fuel Cell Combined Heat and Power

    SciTech Connect

    2009-11-01

    TDA Research Inc., in collaboration with FuelCell Energy, will develop a new, high-capacity sorbent to remove sulfur from anaerobic digester gas. This technology will enable the production of a nearly sulfur-free biogas to replace natural gas in fuel cell power plants while reducing greenhouse gas emissions from fossil fuels.

  3. Net energy production and emissions mitigation of domestic wastewater treatment system: a comparison of different biogas-sludge use alternatives.

    PubMed

    Chen, Shaoqing; Chen, Bin

    2013-09-01

    Wastewater treatment systems are increasingly designed for the recovery of valuable chemicals and energy in addition to waste stream disposal. Herein, the life-cycle energy production and emissions mitigation of a typical domestic wastewater treatment system were assessed, in which different combinations of biogas use and sludge processing lines for industrial or household applications were considered. The results suggested that the reuse of biogas and sludge was so important in the system's overall energy balance and environmental performance that it may offset the cost in the plant's installation and operation. Combined heat and power and household utilization were two prior options for net energy production, provided an ideal power conversion efficiency and biogas production. The joint application of household biogas use and sludge nutrient processing achieved both high net energy production and significant environmental remediation across all impact categories, representing the optimal tradeoff for domestic wastewater treatment. PMID:23880131

  4. Choosing co-substrates to supplement biogas production from animal slurry--a life cycle assessment of the environmental consequences.

    PubMed

    Croxatto Vega, Giovanna Catalina; ten Hoeve, Marieke; Birkved, Morten; Sommer, Sven G; Bruun, Sander

    2014-11-01

    Biogas production from animal slurry can provide substantial contributions to reach renewable energy targets, yet due to the low methane potential of slurry, biogas plants depend on the addition of co-substrates to make operations profitable. The environmental performance of three underexploited co-substrates, straw, organic household waste and the solid fraction of separated slurry, were assessed against slurry management without biogas production, using LCA methodology. The analysis showed straw, which would have been left on arable fields, to be an environmentally superior co-substrate. Due to its low nutrient content and high methane potential, straw yields the lowest impacts for eutrophication and the highest climate change and fossil depletion savings. Co-substrates diverted from incineration to biogas production had fewer environmental benefits, due to the loss of energy production, which is then produced from conventional fossil fuels. The scenarios can often provide benefits for one impact category while causing impacts in another. PMID:25226057

  5. The local view on the role of plant protection in sustainable agriculture in India.

    PubMed

    Jayaraj, S; Rabindra, R J

    1993-01-01

    Indiscriminate use of chemical insecticides has affected humans and their environment and contributed significantly to reduced productivity of crops. With the increasing realization of the importance of sustainable agriculture, the concept of integrated pest management (IPM) for sustainable agriculture has emerged. In the recent past entomologists and the farmers have identified methods of pest management that are ecologically non-disruptive and stable. Concurrently indigenous crop varieties with resistance to pests and diseases have been developed and cultivated. According to the principle of 'organic farming', several non-chemical methods have become popular among the local farmers. Simple cultural practices like increasing the seed rate to compensate for pest damage, adjusting the time of sowing to avoid pest damage, mulching, intercropping, trap cropping and crop rotation have been found to provide adequate protection from pest damage with no additional cost and without harmful effects on the environment. The age-old method of catch and kill is still being practised by farmers, particularly for cotton. Mechanical methods like the bow trap for control of rats and provision of tin sheets around coconut tree trunks to prevent rats damaging the nuts are still being adopted. The use of botanical materials such as the neem products for pest management has been well received almost all over the world. Biological control using the natural enemies of insect pests has become very popular among the farmers in the 1980s. The farmers who clamoured for chemical pesticides in the 1960s and 1970s are now disillusioned with these poisonous eco-destabilizing substances; they want sensible, biologically rational methods of IPM. Pest surveillance and monitoring play an important role in IPM for sustainable agriculture. PMID:8149820

  6. Impact of biosolids and wastewater effluent application to agricultural land on corticosterone content in lettuce plants.

    PubMed

    Shargil, Dorit; Fine, Pinchas; Gerstl, Zev; Nitsan, Ido; Kurtzman, Daniel

    2016-01-15

    We studied corticosterone occurrence in lettuce plants grown on three biosolids amended soils under irrigation with either tap water or secondary wastewater effluent. Corticosterone was examined as it has possible implications for human health. It is a major glucocorticoid, and as such has an effect on regulation of metabolism, immune functions and stress response. The plants were grown in 220-L lysimeters packed with 3 soils which represent a wide range of physicochemical properties. Lettuce was grown in cycles (two in summer and two in winter) during 3 years, and in every spring season the sludges were re-applied. Corticosterone was quantified using ELISA and LCMS, and was found in the biosolids, tap water, wastewater effluent and lettuce plants. The respective ranges of concentrations were: 11-92 ng g(-1), 0.5-1.6 ng L(-1), 4.2-4.7 ng L(-1); and 1-900 ng g(-1) dry weight. A positive relationship was found between corticosterone concentrations in winter-grown lettuces and the plants fresh weight. The corticosterone content of the plants did not correspond with either the type of irrigation water or the biosolids type and rate of application or the soil properties. PMID:26437348

  7. High throughput imaging and analysis for biological interpretation of agricultural plants and environmental interaction

    NASA Astrophysics Data System (ADS)

    Hong, Hyundae; Benac, Jasenka; Riggsbee, Daniel; Koutsky, Keith

    2014-03-01

    High throughput (HT) phenotyping of crops is essential to increase yield in environments deteriorated by climate change. The controlled environment of a greenhouse offers an ideal platform to study the genotype to phenotype linkages for crop screening. Advanced imaging technologies are used to study plants' responses to resource limitations such as water and nutrient deficiency. Advanced imaging technologies coupled with automation make HT phenotyping in the greenhouse not only feasible, but practical. Monsanto has a state of the art automated greenhouse (AGH) facility. Handling of the soil, pots water and nutrients are all completely automated. Images of the plants are acquired by multiple hyperspectral and broadband cameras. The hyperspectral cameras cover wavelengths from visible light through short wave infra-red (SWIR). Inhouse developed software analyzes the images to measure plant morphological and biochemical properties. We measure phenotypic metrics like plant area, height, and width as well as biomass. Hyperspectral imaging allows us to measure biochemcical metrics such as chlorophyll, anthocyanin, and foliar water content. The last 4 years of AGH operations on crops like corn, soybean, and cotton have demonstrated successful application of imaging and analysis technologies for high throughput plant phenotyping. Using HT phenotyping, scientists have been showing strong correlations to environmental conditions, such as water and nutrient deficits, as well as the ability to tease apart distinct differences in the genetic backgrounds of crops.

  8. Microbiological removal of hydrogen sulfide from biogas by means of a separate biofilter system: experience with technical operation.

    PubMed

    Schieder, D; Quicker, P; Schneider, R; Winter, H; Prechtl, S; Faulstich, M

    2003-01-01

    The "BIO-Sulfex" biofilter of ATZ-EVUS removes hydrogen sulfide from biogas in a biological way. Hydrogen sulfide causes massive problems during power generation from biogas in a power plant, e.g. corrosion of engines and heat exchangers, and thus causes frequent and therefore expensive engine oil changes. The BIO-Sulfex module is placed between the digester and the power-plant and warrants a cost-effective, reliable and fully biological desulfurization. In the cleaned gas concentrations of less than 100 ppm can be achieved. Power-plant manufacturers usually demand less than 500 or less than 200 ppm. At present, several plants with biogas flow rates between 20 and 350 m3/h are in operation. PMID:14531443

  9. Biogas Production from Sugarcane Waste: Assessment on Kinetic Challenges for Process Designing

    PubMed Central

    Janke, Leandro; Leite, Athaydes; Nikolausz, Marcell; Schmidt, Thomas; Liebetrau, Jan; Nelles, Michael; Stinner, Walter

    2015-01-01

    Biogas production from sugarcane waste has large potential for energy generation, however, to enable the optimization of the anaerobic digestion (AD) process each substrate characteristic should be carefully evaluated. In this study, the kinetic challenges for biogas production from different types of sugarcane waste were assessed. Samples of vinasse, filter cake, bagasse, and straw were analyzed in terms of total and volatile solids, chemical oxygen demand, macronutrients, trace elements, and nutritional value. Biochemical methane potential assays were performed to evaluate the energy potential of the substrates according to different types of sugarcane plants. Methane yields varied considerably (5–181 Nm3·tonFM−1), mainly due to the different substrate characteristics and sugar and/or ethanol production processes. Therefore, for the optimization of AD on a large-scale, continuous stirred-tank reactor with long hydraulic retention times (>35 days) should be used for biogas production from bagasse and straw, coupled with pre-treatment process to enhance the degradation of the fibrous carbohydrates. Biomass immobilization systems are recommended in case vinasse is used as substrate, due to its low solid content, while filter cake could complement the biogas production from vinasse during the sugarcane offseason, providing a higher utilization of the biogas system during the entire year. PMID:26404248

  10. Biogas Production from Sugarcane Waste: Assessment on Kinetic Challenges for Process Designing.

    PubMed

    Janke, Leandro; Leite, Athaydes; Nikolausz, Marcell; Schmidt, Thomas; Liebetrau, Jan; Nelles, Michael; Stinner, Walter

    2015-01-01

    Biogas production from sugarcane waste has large potential for energy generation, however, to enable the optimization of the anaerobic digestion (AD) process each substrate characteristic should be carefully evaluated. In this study, the kinetic challenges for biogas production from different types of sugarcane waste were assessed. Samples of vinasse, filter cake, bagasse, and straw were analyzed in terms of total and volatile solids, chemical oxygen demand, macronutrients, trace elements, and nutritional value. Biochemical methane potential assays were performed to evaluate the energy potential of the substrates according to different types of sugarcane plants. Methane yields varied considerably (5-181 Nm³·tonFM(-1)), mainly due to the different substrate characteristics and sugar and/or ethanol production processes. Therefore, for the optimization of AD on a large-scale, continuous stirred-tank reactor with long hydraulic retention times (>35 days) should be used for biogas production from bagasse and straw, coupled with pre-treatment process to enhance the degradation of the fibrous carbohydrates. Biomass immobilization systems are recommended in case vinasse is used as substrate, due to its low solid content, while filter cake could complement the biogas production from vinasse during the sugarcane offseason, providing a higher utilization of the biogas system during the entire year. PMID:26404248

  11. Utilization of by-products from ethanol production as substrate for biogas production.

    PubMed

    De Paoli, F; Bauer, A; Leonhartsberger, C; Amon, B; Amon, T

    2011-06-01

    The aims of this work were to determine the specific biogas yields of steam-exploded sugarcane straw and bagasse as well as to estimate their energy potential under Brazilian conditions. Steam-explosion was carried out under different time and temperature conditions. The specific biogas yields were analyzed in batch-tests according to VDI 4630. Results have shown that steam-explosion pre-treatment increased the specific biogas yields of straw and bagasse significantly compared to the untreated material. The utilization of these by-products can contribute to 5% of the total energy consumption and thereby higher energy independence in Brazil. Further efforts in defining the optimum pretreatment conditions with steam-explosion as well as implementing this technology in large scale plants should be made. PMID:21481586

  12. Industrial scale garage-type dry fermentation of municipal solid waste to biogas.

    PubMed

    Qian, M Y; Li, R H; Li, J; Wedwitschka, H; Nelles, M; Stinner, W; Zhou, H J

    2016-10-01

    The objectives of this study was to through monitoring the 1st industrial scale garage-type dry fermentation (GTDF) MSW biogas plant in Bin County, Harbin City, Heilongjiang Province, China, to investigate its anaerobic digestion (AD) performance and the stability of process. After a monitoring period of 180days, the results showed that the volumetric biogas production of the digesters and percolate tank was 0.72 and 2.22m(3) (m(3)d)(-1), respectively, and the specific biogas yield of the feedstock was about 270m(3)CH4tVS(-1), which indicated that the GTDF is appropriate for the Chinese MSW. This paper also raised some problems aimed at improving the process stability and AD efficiency. PMID:26970693

  13. Trace element biogeochemistry in the soil-water-plant system of a temperate agricultural soil amended with different biochars.

    PubMed

    Kloss, Stefanie; Zehetner, Franz; Buecker, Jannis; Oburger, Eva; Wenzel, Walter W; Enders, Akio; Lehmann, Johannes; Soja, Gerhard

    2015-03-01

    Various biochar (BC) types have been investigated as soil amendment; however, information on their effects on trace element (TE) biogeochemistry in the soil-water-plant system is still scarce. In the present study, we determined aqua-regia (AR) and water-extractable TEs of four BC types (woodchips (WC), wheat straw (WS), vineyard pruning (VP), pyrolyzed at 525 °C, of which VP was also pyrolyzed at 400 °C) and studied their effects on TE concentrations in leachates and mustard (Sinapis alba L.) tissue in a greenhouse pot experiment. We used an acidic, sandy agricultural soil and a BC application rate of 3% (w/w). Our results show that contents and extractability of TEs in the BCs and effectuated changes of TE biogeochemistry in the soil-water-plant system strongly varied among the different BC types. High AR-digestable Cu was found in VP and high B contents in WC. WS had the highest impact on TEs in leachates showing increased concentrations of As, Cd, Mo, and Se, whereas WC application resulted in enhanced leaching of B. All BC types increased Mo and decreased Cu concentrations in the plant tissue; however, they showed diverging effects on Cu in the leachates with decreased concentrations for WC and WS, but increased concentrations for both VPs. Our results demonstrate that BCs may release TEs into the soil-water-plant system. A BC-induced liming effect in acidic soils may lead to decreased plant uptake of cationic TEs, including Pb and Cd, but may enhance the mobility of anionic TEs like Mo and As. We also found that BCs with high salt contents (e.g., straw-based BCs) may lead to increased mobility of both anionic and cationic TEs in the short term. PMID:25315931

  14. Pollinating flies (Diptera): A major contribution to plant diversity and agricultural production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Diptera are one of the three largest and most diverse animal groups of the world. As an often neglected, but important group of pollinators, they play a significant role in agrobiodiversity and biodiversity of plants everywhere. Flies are present in almost all habitats and biomes and for many food p...

  15. An Analysis of Occupational Titles and Competencies Needed in Agricultural Food Products Processing Plants.

    ERIC Educational Resources Information Center

    Smeltz, LeRoy C.

    To identify, rate, and cluster groups of competencies and occupational titles at entry and advance levels for occupations in five food products commodity areas, data were collected by interviews with personnel managers in 25 Pennsylvania food processing plants. Some findings were: (1) There were meaningful competency factor and occupational title…

  16. Introduction to the USDA-Agricultural Research Service Poisonous Plant Research Laboratory Special Rangelands Issue

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The USDA-ARS Poisonous Plant Research Labortory (PPRL) in Logan, UT will sponsor an edition of the magazine Rangelands. This paper provides a brief history and overview of the PPRL, mission statement, research objectives by CRIS, and the disciplines involved in the research....

  17. A comparison of the herbicide tolerances of rare and common plants in an agricultural landscape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Declining plant biodiversity in agroecosystems has often been attributed to escalating use of chemical herbicides, but other changes in farming practice including the clearing of semi-natural habitat fragments confound the influence of herbicides. In this paper, we introduce a new approach to evalua...

  18. Integrating Phytoextraction and Biofortification: Fungal Accumulation of Selenium in Plant Materials from Phytoremediation of Agricultural Drainage

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The phytomanagement of Se-polluted soil and water is one strategy that may be environmentally sustainable and cost-effective for soils and waters enriched with natural-occurring Se. Several plant species, including Indian mustard (Brassica juncea), pickleweed (Salicornia bigelovii), and other salt/S...

  19. Pivoting from Arabidopsis to wheat to understand how agricultural plants integrate responses to biotic stress

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Here we argue for a research initiative on gene-for-gene (g-f-g) interactions between wheat and its parasites. One aim is to begin a conversation between the disparate communities of plant pathology and entomology. Another is to understand how responses to biotic stress are integrated in an import...

  20. EVALUATION OF OZONE EXPOSURE INDICES FOR RELATING EXPOSURE TO PLANT PRODUCTION AND FOR ESTIMATING AGRICULTURAL LOSSES

    EPA Science Inventory

    The objective of the report is: (1) to compare alternative ozone exposure indices that relate well to plant response and are useful in developing future standards for ozone; (2) to estimate standard errors and confidence intervals of regression coefficients and exposure levels of...

  1. Harvesting biogas from wastewater sludge and food waste

    NASA Astrophysics Data System (ADS)

    Chua, K. H.; Cheah, W. L.; Tan, C. F.; Leong, Y. P.

    2013-06-01

    Wastewater sludge and food waste are good source of biogas. Anaerobic treatment of slude and food waste able to produce biogas which is a potential renewable energy source. This study looks into the potential biogas generation and the effects of temperature on biogas generation. A lab scale reactor was used to simulate the biogas generation. The results show that wastewater sludge able to produced upto 44.82 ml biogas/kg of sludge. When mixed with food waste at a ratio of 30:70 (food waste), the biogas generated were 219.07 ml/kg of waste. Anaerobic of food waste alone produced biogas amount to 59.75 ml/kg of food waste. Anaerobic treatment also reduces the volume of waste. The effect of temperature shows that higher temperature produces more biogas than lower temperature.

  2. Comparative analysis of environmental impacts of maize-biogas and photovoltaics on a land use basis

    SciTech Connect

    Graebig, Markus; Fenner, Richard; Bringezu, Stefan

    2010-07-15

    This study aims to stimulate the discussion on how to optimize a sustainable energy mix from an environmental perspective and how to apply existing renewable energy sources in the most efficient way. Ground-mounted photovoltaics (PV) and the maize-biogas-electricity route are compared with regard to their potential to mitigate environmental pressure, assuming that a given agricultural area is available for energy production. Existing life cycle assessment (LCA) studies are taken as a basis to analyse environmental impacts of those technologies in relation to conventional technology for power and heat generation. The life-cycle-wide mitigation potential per area used is calculated for the impact categories non-renewable energy input, green house gas (GHG) emissions, acidification and eutrophication. The environmental performance of each system depends on the scenario that is assumed for end energy use (electricity and heat supply have been contemplated). In all scenarios under consideration, PV turns out to be superior to biogas in almost all studied impact categories. Even when maize is used for electricity production in connection with very efficient heat usage, and reduced PV performance is assumed to account for intermittence, PV can still mitigate about four times the amount of green house gas emissions and non-renewable energy input compared to maize-biogas. Soil erosion, which can be entirely avoided with PV, exceeds soil renewal rates roughly 20-fold on maize fields. Regarding the overall Eco-indicator 99 (H) score under most favourable assumptions for the maize-biogas route, PV has still a more than 100% higher potential to mitigate environmental burden. At present, the key advantages of biogas are its price and its availability without intermittence. In the long run, and with respect to more efficient land use, biogas might preferably be produced from organic waste or manure, whereas PV should be integrated into buildings and infrastructures. (author)

  3. Cavitation for improved sludge conversion into biogas

    NASA Astrophysics Data System (ADS)

    Stoop, A. H.; Bakker, T. W.; Kramer, H. J. M.

    2015-12-01

    In several studies the beneficial influence of pre-treatment of waste activated sludge with cavitation on the biogas production was demonstrated. It is however, still not fully certain whether this effect should be mainly contributed to an increase in conversion rate of organics into biogas by anaerobic bacteria, and how much cavitation increases the total biogas yield. An increase in yield is only the case if cavitation can further disrupt otherwise inaccessible cell membrane structures and long chain organic molecules. In this study the influence of hydrodynamic cavitation on sludge that was already digested for 30 days was investigated. The total biogas yield could indeed be increased. The effect of the backpressure behind the venturi tube on the yield could not yet be established.

  4. Energy integrated dairy farm system in Georgia: Technical manual, Mathis/P and M Dairy Farm, Social Circle, Georgia. [Cogeneration using biogas; heat recovery

    SciTech Connect

    Walsh, J.L. Jr.; Ross, C.C.; Lamade, R.M.

    1986-09-01

    This manual describes a project sponsored to optimize energy generation and utilization in the agricultural or food processing industry. The particular project involves the Mathis/P and M Dairy Farm located in Social Circle, Georgia (about 60 miles east of Atlanta). The farm is designed for a 550 milking cow herd and produces certified raw milk for sale to a processing plant located in Atlanta. The project converted the Mathis/P and and M Dairy into an energy integrated dairy farm system (EIDFS) in which the interaction of the subsystems and components are modified such that the energy resources of the farm are optimized. This manual is a description of the system, subsystems and components composing the Mathis EIDFS and is primarily intended for farmers, extension agents, and equipment manufacturers who might be involved in future EIDFS projects. Cogeneration using biogas from manures and heat recovery from the refrigeration machinery were among the options chosen.

  5. White Earth Biomass/Biogas Feasibility Study

    SciTech Connect

    Triplett, Michael

    2015-03-12

    The White Earth Nation examined the feasibility of cost savings and fossil energy reduction through the installation of biogas/biomass boiler at the tribal casino. The study rejected biogas options due to availability and site constraints, but found a favorable environment for technical and financial feasibility of installing a 5 MMBtu hot water boiler system to offset 60-70 percent of current fuel oil and propane usage.

  6. Alkaloids from marine sponges as stimulators of initial stages of development of agricultural plants.

    PubMed

    Anisimov, Mikhail M; Chaikina, Elena L; Utkina, Natalia K

    2014-04-01

    Damirone A (1), damirone B (2), makaluvamine G (3), debromohymenialdisine (4), and dibromoagelaspongin (5) were examined for their ability to stimulate growth of seedling roots of barley (Hordeum vulgare L.), buckwheat (Fagopyrum esculentum Moench), corn (Zea mays L.), soy (Glycine max (L.) Merr.}, and wheat (Triticum aestivum L.). It was shown that the stimulatory effects depend on the chemical structure of the alkaloids and on the plant species. Compounds 1, 3, and 4 are efficient for growth of seedling roots of barley, compounds 2-5, at different concentrations, stimulate growth of buckwheat roots, and compound 5 stimulates growth of wheat roots. These compounds can be recommended for field study as plant growth stimulators. PMID:24868854

  7. Influence of Merosesquiterpenoids from Marine Sponges on Seedling Root Growth of Agricultural Plants.

    PubMed

    Chaikina, Elena L; Utkina, Natalia K; Anisimov, Mikhail M

    2016-01-01

    The impact of the merosesquiterpenoids avarol (1), avarone (2), 18-methylaminoavarone (3), melemeleone A (4), isospongiaquinone (5), ilimaquinone (6), and smenoquinone (7), isolated from marine sponges of the Dictyoceratida order, was studied on the root growth of seedlings of buckwheat (Fagopyrumesculentum Moench), wheat (Triticumaestivum L.), soy (Glycine max (L.) Merr.), and barley (Hordeumvulgare L.). Compounds 2and 6 were effective for the root growth of wheat seedlings, compound 3 stimulated the root growth of seedlings of buckwheat and soy, compound 4 affected the roots of barley seedlings, and compound 5 stimulated the root growth of seedlings of buckwheat and barley. Compounds 1 and 7 showed no activity on the root growth of the seedlings of any of the studied plants. The stimulatory effect depends on the chemical structure of the compounds and the type of crop plant. PMID:26996006

  8. Contaminated agricultural soils: Trace-elements speciation their phytoavailability and their uptake by flax plants

    NASA Astrophysics Data System (ADS)

    Legras, M.; Kharbouch, F.; Giron, F.; Bert, F.; Llorens, J.-M.

    2003-05-01

    Flax seeds are used in animal food because of their high content in Omega 3. A number of trace-elements (TEs) - essential as micronutrients, however toxic at supraoptimal concentrations - can accumulate in this plant at quantities incompatible with their introduction in food chain. In order to control this risk and evaluate the uptake of TEs, it is necessary to assess the contents of various species of TEs in soils and plants (each organ and total contents). We were mainly interested in evaluating the availability of Cd, Cu, Ni, Pb and Zn in soils on which flax were grown. Two situations have been compared: the first corresponds to fields into which some sewage sludge were brought in agronomie doses and the second corresponds to plots of land irrigated by waste water over a 100 years period. We are currently performing TEs extractions from soils and plants using different methods : the data of sequential and total extractions (assisted by microwaves) are presented. We have studied two flax varieties in four stages of culture (sowing, stage 10cm. tlowering, maturation). The content, speciation of TEs in soils as well as their transfer in flax seeds are discussed.

  9. Biogas production experimental research using algae.

    PubMed

    Baltrėnas, Pranas; Misevičius, Antonas

    2015-01-01

    The current study is on the the use of macro-algae as feedstock for biogas production. Three types of macro-algae, Cladophora glomerata (CG), Chara fragilis (CF), and Spirogyra neglecta (SN), were chosen for this research. The experimental studies on biogas production were carried out with these algae in a batch bioreactor. In the bioreactor was maintained 35 ± 1°C temperature. The results showed that the most appropriate macro-algae for biogas production are Spirogyra neglecta (SN) and Cladophora glomerata (CG). The average amount of biogas obtained from the processing of SN - 0.23 m(3)/m(3)d, CG - 0.20 m(3)/m(3)d, and CF - 0.12 m(3)/m(3)d. Considering the concentration of methane obtained during the processing of SN and CG, which after eight days and until the end of the experiment exceeded 60%, it can be claimed that biogas produced using these algae is valuable. When processing CF, the concentration of methane reached the level of 50% only by the final day of the experiment, which indicates that this alga is less suitable for biogas production. PMID:25859392

  10. Characterization of a biogas-producing microbial community by short-read next generation DNA sequencing

    PubMed Central

    2012-01-01

    microbiological diversity and the regulatory role of the hydrogen metabolism appear to be the driving forces optimizing biogas-producing microbial communities. The findings may allow a rational design of these communities to promote greater efficacy in large-scale practical systems. The composition of an optimal biogas-producing consortium can be determined through the use of this approach, and this systematic methodology allows the design of the optimal microbial community structure for any biogas plant. In this way, metagenomic studies can contribute to significant progress in the efficacy and economic improvement of biogas production. PMID:22673110

  11. Impact of acid effluent from Kawah Ijen crater lake on irrigated agricultural soils: Soil chemical processes and plant uptake

    NASA Astrophysics Data System (ADS)

    van Rotterdam-Los, A. M. D.; Heikens, A.; Vriend, S. P.; van Bergen, M. J.; van Gaans, P. F. M.

    2008-12-01

    Volcanogenic contamination of irrigation water, caused by effluent from the hyperacid Ijen crater lake, has severely affected the properties of agricultural soils in East Java, Indonesia. From a comparison of acidified topsoil with subsoil and with top- and subsoil in a reference area, we identified processes responsible for changes in soil and soil solution chemistry induced by acid irrigation water, with emphasis on the nutrients Ca, Mg, Fe, and Mn, and on Al, which may become phytotoxic under acid conditions in soils. Compositional data for bulk soil composition and selective extractions with 1 M KCl and 0.2 M acid ammonium oxalate are used in a mass balance approach to specify element fluxes, including uptake by rice plants. The results show that input via irrigation water has produced an increase in the total aluminum content in the affected topsoil, which is of the same order of magnitude as the increase in labile Al. High bioavailability of Al, as reflected by concentrations in KCl extracts, is consistent with elevated concentrations observed in rice plants. In contrast, and despite the high input via irrigation water, Ca and Mg concentrations have decreased in all measured soil fractions through dissolution of amorphous phases and minerals, and through competition of Al for adsorption sites on the exchange complex and plant roots. Strong leaching is also evident for Fe and especially Mn. In terms of the overall mass balance of the topsoil, plant uptake of Al, Ca, Fe, Mg and Mn is negligible. If the use of acid irrigation would be stopped and the soil pH were to increase to values above 4.5, the observed phytotoxicity of Al will be halted. However, crops may then become fully dependent on the input from irrigation water or fertilizer for essential elements, due to the previous removal from the topsoil through leaching.

  12. Effects of biogas digestate and cattle slurry application on greenhouse gas emissions from grasslands on organic soils

    NASA Astrophysics Data System (ADS)

    Eickenscheidt, Tim; Freibauer, Annette; Heinichen, Jan; Augustin, Jürgen; Drösler, Matthias

    2014-05-01

    The change in the German energy policy resulted in a strong development of biogas plants in Germany. As a consequence, drained peatlands are increasingly used to satisfy the rising demand for fermentative substrates. In return high amounts of nutrient-rich digestates are used as organic fertilizer to maintain soil fertility and crop yield. It is well known that organic fertilization enhances nitrous oxide (N2O) emissions from managed grasslands, especially in south Germany, a region with frequently frost-thaw cycles. Additionally drained organic soils are considered as hotspots of GHG emissions including N2O. Our study addressed the question to what extent biogas digestate and cattle slurry application alters N2O and methane (CH4) fluxes and how different contents of soil organic matter (SOM) promote the production of GHG. The study was conducted at two areas within one grassland parcel, which differed in their soil organic carbon (SOC) contents (10% versus 17%). At each area (named Corg medium and Corg high) three sites were established: One was fertilized five times with biogas digestate, the second five times with cattle slurry and the third site served as control without fertilization. For each treatment, the fluxes of N2O and CH4 were measured over two years using the closed chamber method. Significantly higher short term (16 days) N2O fluxes after fertilization with digestate compared to slurry could only be found in one out of four fertilisation events. However, on an annual basis the application of biogas digestate significantly enhanced the N2O fluxes compared to the application of cattle slurry. Furthermore, N2O fluxes from the Corghigh site significantly exceeded N2O fluxes from the Corg medium sites. Annual cumulative emissions ranged from 0.91 ± 0.49 kg N ha-1 yr-1 to 3.14 ± 0.91 kg N ha-1 yr-1, reflecting the lower end of literature values from other organic soils and corresponding more to those reported from grasslands on mineral soils in Germany

  13. Association of N2-fixing cyanobacteria and plants: towards novel symbioses of agricultural importance

    SciTech Connect

    Elhai, Jeff

    2001-06-25

    Some nitrogen-fixing cyanobacteria are able to form symbioses with a wide variety of plants. Nostoc 2S9B is unusual in its ability to infect the roots of wheat, raising the prospect of a productive association with an important crop plant. The goal of the project was to lay the groundwork for the use of novel associations between Nostoc and crops of agronomic importance, thereby reducing our reliance on nitrogenous fertilizer. Nostoc 2S9B was found to enter roots through mechanical damage of roots and reside primarily in intercellular spaces. The strain could also be incorporated into wheat calli grown in tissue culture. In both cases, the rate of nitrogen fixation by the cyanobacterium was higher than that of the same strain grown with no plant present. Artificial nodules induced by the action of hormone 2,4D were readily infected by Nostoc 2S9B, and the cyanobacteria within such nodules fixed nitrogen under fully aerobic conditions. The nitrogen fixed was shown to be incorporated into the growing wheat seedlings. Nostoc thus differs from other bacteria in its ability to fix nitrogen in para-nodules without need for artificially microaerobic conditions. It would be useful to introduce foreign DNA into Nostoc 2S9B in order to make defined mutations to understand the genetic basis of its ability to infect wheat and to create strains that might facilitate the study of the infection process. Transfer of DNA into the cyanobacterium appears to be limited by the presence of four restriction enzymes, with recognition sequences the same as BamHI, BglI, BsaHI, and Tth111I. Genes encoding methyltransferases that protect DNA against these four enzymes have been cloned into helper plasmids to allow transfer of DNA from E. coli to Nostoc 2S9B.

  14. Enhancement of the Initial Growth Rate of Agricultural Plants by Using Static Magnetic Fields.

    PubMed

    Kim, Seung C; Mason, Alex; Im, Wooseok

    2016-01-01

    Electronic devices and high-voltage wires induce magnetic fields. A magnetic field of 1,300-2,500 Gauss (0.2 Tesla) was applied to Petri dishes containing seeds of Garden Balsam (Impatiens balsamina), Mizuna (Brassica rapa var. japonica), Komatsuna (Brassica rapa var. perviridis), and Mescluns (Lepidium sativum). We applied magnets under the culture dish. During the 4 days of application, we observed that the stem and root length increased. The group subjected to magnetic field treatment (n = 10) showed a 1.4 times faster rate of growth compared with the control group (n = 11) in a total of 8 days (p <0.0005). This rate is 20% higher than that reported in previous studies. The tubulin complex lines did not have connecting points, but connecting points occur upon the application of magnets. This shows complete difference from the control, which means abnormal arrangements. However, the exact cause remains unclear. These results of growth enhancement of applying magnets suggest that it is possible to enhance the growth rate, increase productivity, or control the speed of germination of plants by applying static magnetic fields. Also, magnetic fields can cause physiological changes in plant cells and can induce growth. Therefore, stimulation with a magnetic field can have possible effects that are similar to those of chemical fertilizers, which means that the use of fertilizers can be avoided. PMID:27500712

  15. Assessment of heavy metal accumulation in macrophyte, agricultural soil, and crop plants adjacent to discharge zone of sponge iron factory

    NASA Astrophysics Data System (ADS)

    Gupta, S.; Nayek, S.; Saha, R. N.; Satpati, S.

    2008-08-01

    The present study deals with the characterization of effluent released from sponge iron industries and distribution of heavy metals in soil and macrophytes near to effluent discharge channel. Apart from this, accumulation of heavy metals in nearby soil and vegetation system irrigated with effluent-contaminated water is also the subject of this study. Physico-chemical analysis of effluent reveals that the concentration of total suspended solids (TSS), total hardness (TH), iron (Fe2+), and oil and grease are greater than the IS (1981) norms for discharge of water into inland water body. The soil along the sides of the effluent channel also shows higher concentration of heavy metals than the background soil. The enrichment of the heavy metals are in the order of Chromium (Cr) > Iron (Fe) > Manganese (Mn) > Zinc (Zn) > Copper (Cu) > Cadmium (Cd). Macrophytes growing along the sides of the effluent channel also show significant accumulation of heavy metals almost in the same order as accumulated in soil. Higher uptake of heavy metals by these varieties reveals that these species can be used for future phytoremediation. The effluent as well as contaminated water is extensively used for irrigation for growing vegetables like tomato ( Lycopersicon esculatum) in the surrounding areas. Heavy metal accumulation in this agricultural soil are in the sequence of Cr > Fe > Mn > Zn > Cu > Cd. More or less similar type of accumulation pattern are also found in tomato plants except Fe and Zn exceeding Cr and Mn. Transfer Factor of heavy metals from soil to tomato plants (TFS) shows average value of <1, suggesting less uptake of heavy metals from soil. Among the plant parts studied, fruit shows least accumulation. Although tomato plants show some phenotypic changes, the survival of tomato plants as well as least accumulation of metals in fruit reveals their tolerance to heavy metals. Therefore it may be suggested that this plant can be grown successfully in the heavy metal

  16. Agricultural Biodiversity.

    ERIC Educational Resources Information Center

    Postance, Jim

    1998-01-01

    The extinction of farm animals and crops is rarely brought up during discussions of endangered species and biodiversity; however, the loss of diversity in crops and livestock threatens the sustainability of agriculture. Presents three activities: (1) "The Colors of Diversity"; (2) "Biodiversity among Animals"; and (3) "Heirloom Plants." Discusses…

  17. Principles of Biomedical Agriculture Applied to the Plant Family Theaceae To Identify Novel Interventions for Cancer Prevention and Control.

    PubMed

    Wang, Yijun; Yang, Yunqiu; Wei, Chaoling; Wan, Xiaochun; Thompson, Henry J

    2016-04-13

    Plant materials from the family Theaceae have been used for over a thousand years as integral components within the food systems of many globally distributed cultures and to treat a variety of human ailments. These markedly different uses remain of considerable interest in the 21st century. This perspective draws heavily from the agricultural and biomedical literature published using plant materials from the genus Camellia. Our objective is to provide a rationale and framework for broadening the scope of investigation of genera and species within Theaceae beyond Camellia sinensis to accelerate the development of a new generation of Theaceae-based pharmaceuticals/nutraceuticals and the more general enhancement of the food supply with Theaceae-containing products that affect the development of chronic diseases such as cancer. This will require a concerted effort to systematically capitalize on the rapidly growing knowledge of germplasm resources within Theaceae using metabolomic profiling in combination with in vivo and in vitro approaches. The successful translation of this research into products that affect human health will be facilitated by recognition of the agronomic factors that are critical in making hot water infusions generically referred to as tea as well as food products containing ground leaf powders. PMID:27011038

  18. Environmental economics reality check: a case study of the Abanico Medicinal Plant and Organic Agriculture Microenterprise Project.

    PubMed

    Isla, Ana; Thompson, Shirley

    2003-01-01

    This paper presents a case study of the Abanico Medicinal Plant and Organic Agriculture Microenterprise Project in the Arenal Conservation Area, Costa Rica. Microenterprise is the Sustainable Development and the Women in Development model for gender equity and environment of the World Bank, International Monetary Fund and large non-government organizations, like the World Wildlife Fund-Canada. The authors of this paper argue that debt-for-nature investment in microenterprise and ecological economic models are not distinct from neoclassical economic and development models that created the environmental, social and cultural crises in the first place. This case study shows that the world market accommodates only one model of development: unsustainable export-oriented production based on flexible labour markets, low wages, indebtedness and low cost production. Working standards in those micro-enterprises are eroded due to many factors,including indebtedness. What happened at a national level in non-industrial countries with the international debt crisis is now mirrored in individual indebtedness through microenterprise. Is current development policy creating a new form of indentured servitude? Medicinal plants, prior to commodification, were a source of women's power and upon commodification in international development projects, are the source of their exploitation. PMID:12859005

  19. Efficiency and biotechnological aspects of biogas production from microalgal substrates.

    PubMed

    Klassen, Viktor; Blifernez-Klassen, Olga; Wobbe, Lutz; Schlüter, Andreas; Kruse, Olaf; Mussgnug, Jan H

    2016-09-20

    Photosynthetic organisms like plants and algae can harvest, convert, and store solar energy and thus represent readily available sources for renewable biofuels production on a domestic or industrial scale. Anaerobic digestion (AD) of the organic biomass yields biogas, containing methane and carbon dioxide as major constituents. Combustion of the biogas or purification of the energy-rich methane fraction can be applied to provide electricity or fuel. AD procedures have been applied for several decades with organic waste, animal products, or higher plants and more recently, utilization of photosynthetic algae as substrates have gained considerable research interest. To provide an overview of recent research efforts made to characterize the AD process of microalgal biomass, we present extended summaries of experimentally determined biochemical methane potentials (BMP), biomass pretreatment options and digestion strategies in this article. We conclude that cultivation options, biomass composition and time of harvesting, application of biomass pretreatment strategies, and parameters of the digestion process are all important factors, which can significantly affect the AD process efficiency. The transition from batch to continuous microalgal biomass digestion trials, accompanied by state-of-the-art analytical techniques, is now in demand to refine the assessments of the overall process feasibility. PMID:27449486

  20. Energy Economics of Farm Biogas in Cold Climates

    SciTech Connect

    Pillay, Pragasen; Grimberg, Stefan; Powers, Susan E

    2012-10-24

    Anaerobic digestion of farm and dairy waste has been shown to be capital intensive. One way to improve digester economics is to co-digest high-energy substrates together with the dairy manure. Cheese whey for example represents a high-energy substrate that is generated during cheese manufacture. There are currently no quantitative tools available that predict performance of co-digestion farm systems. The goal of this project was to develop a mathematical tool that would (1) predict the impact of co-digestion and (2) determine the best use of the generated biogas for a cheese manufacturing plant. Two models were developed that separately could be used to meet both goals of the project. Given current pricing structures of the most economical use of the generated biogas at the cheese manufacturing plant was as a replacement of fuel oil to generate heat. The developed digester model accurately predicted the performance of 26 farm digesters operating in the North Eastern U.S.

  1. Gasification of agricultural residues in a demonstrative plant: Vine pruning and rice husks.

    PubMed

    Biagini, Enrico; Barontini, Federica; Tognotti, Leonardo

    2015-10-01

    Tests with vine pruning and rice husks were carried out in a demonstrative downdraft gasifier (350 kW), to prove the reactor operability, quantify the plant efficiency, and thus extend the range of potential energy feedstocks. Pressure drops, syngas flow rate and composition were monitored to study the material and energy balances, and performance indexes. Interesting results were obtained for vine pruning (syngas heating value 5.7 MJ/m(3), equivalent ratio 0.26, cold gas efficiency 65%, power efficiency 21%), while poorer values were obtained for rice husks (syngas heating value 2.5-3.8 MJ/m(3), equivalent ratio 0.4, cold gas efficiency 31-42%, power efficiency 10-13%). The work contains also a comparison with previous results (wood pellets, corn cobs, Miscanthus) for defining an operating diagram, based on material density and particle size and shape, and the critical zones (reactor obstruction, bridging, no bed buildup, combustion regime). PMID:26183923

  2. Counteracting foaming caused by lipids or proteins in biogas reactors using rapeseed oil or oleic acid as antifoaming agents.

    PubMed

    Kougias, P G; Boe, K; Einarsdottir, E S; Angelidaki, I

    2015-08-01

    Foaming is one of the major operational problems in biogas plants, and dealing with foaming incidents is still based on empirical practices. Various types of antifoams are used arbitrarily to combat foaming in biogas plants, but without any scientific support this action can lead to serious deterioration of the methanogenic process. Many commercial antifoams are derivatives of fatty acids or oils. However, it is well known that lipids can induce foaming in manure based biogas plants. This study aimed to elucidate the effect of rapeseed oil and oleic acid on foam reduction and process performance in biogas reactors fed with protein or lipid rich substrates. The results showed that both antifoams efficiently suppressed foaming. Moreover rapeseed oil resulted in stimulation of the biogas production. Finally, it was reckoned that the chemical structure of lipids, and more specifically their carboxylic ends, is responsible for their foam promoting or foam counteracting behaviour. Thus, it was concluded that the fatty acids and oils could suppress foaming, while salt of fatty acids could generate foam. PMID:25978353

  3. CO2 abatement costs of greenhouse gas (GHG) mitigation by different biogas conversion pathways.

    PubMed

    Rehl, T; Müller, J

    2013-01-15

    Biogas will be of increasing importance in the future as a factor in reducing greenhouse gas emissions cost-efficiently by the optimal use of available resources and technologies. The goal of this study was to identify the most ecological and economical use of a given resource (organic waste from residential, commercial and industry sectors) using one specific treatment technology (anaerobic digestion) but applying different energy conversion technologies. Average and marginal abatement costs were calculated based on Life Cycle Cost (LCC) and Life Cycle Assessment (LCA) methodologies. Eight new biogas systems producing electricity, heat, gas or automotive fuel were analyzed in order to identify the most cost-efficient way of reducing GHG emissions. A system using a combined heat and power station (which is connected to waste treatment and digestion operation facilities and located nearby potential residential, commercial or industrial heat users) was found to be the most cost-efficient biogas technology for reducing GHG emissions. Up to € 198 per tonne of CO(2) equivalents can be saved by replacing the "business as usual" systems based on fossil resources with ones based on biogas. Limited gas injection (desulfurized and dried biogas, without compression and upgrading) into the gas grid can also be a viable option with an abatement cost saving of € 72 per tonne of CO(2) equivalents, while a heating plant with a district heating grid or a system based on biogas results in higher abatement costs (€ 267 and € 270 per tonne CO(2) eq). Results from all systems are significantly influenced by whether average or marginal data are used as a reference. Beside that energy efficiency, the reference system that was replaced and the by-products as well as feedstock and investment costs were identified to be parameters with major impacts on abatement costs. The quantitative analysis was completed by a discussion of the role that abatement cost methodology can play in

  4. An economic analysis of the electricity generation potential from biogas resources in the state of Indiana

    NASA Astrophysics Data System (ADS)

    Giraldo, Juan S.

    Anaerobic digestion is a process that is a common part of organic waste management systems and is used in concentrated animal feeding operations (CAFOs), wastewater treatment plants (WWTPs), and municipal solid waste (MSW) landfills. The process produces biogas, which contains methane, and it can be burned to generate electricity. Previous reports have indicated that based on the availability of feedstocks there is a large potential for biogas production and use for electricity generation in the state of Indiana. However, these reports varied in their consideration of important factors that affect the technical and economic feasibility of being able to develop the resources available. The goal of this thesis is to make a more targeted assessment of the electricity generation potential from biogas resources at CAFOs, WWTPs, and MSW landfills in Indiana. A capital budgeting model is used to estimate the net present value (NPV) of biogas electricity projects at facilities that are identified as technically suitable. A statewide estimate of the potential generation capacity is made by estimating the number of facilities that could profitably undertake a biogas electricity project. In addition this thesis explored the impact that different incentive policies would have on the economic viability of these projects. The results indicated that the electricity generation potential is much smaller when technical and economic factors are taken into account in addition to feedstock availability. In particular it was found that projects at hog farms are unlikely to be economically feasible in the present even when financial incentives are considered. In total, 47.94 MW of potential generating capacity is estimated from biogas production at CAFOs, WWTPs, and MSW landfills. Though results indicated that 37.10 MW of capacity are economically feasible under current operating conditions, sensitivity analysis reveals that these projects are very sensitive to capital cost assumptions

  5. Feasibility study for retrofitting biogas cogeneration systems to district heating in South Korea.

    PubMed

    Chung, Mo; Park, Hwa-Choon

    2015-08-01

    A feasibility study was performed to assess the technical and economic merits of retrofitting biogas-based cogeneration systems to district heating networks. Three district heating plants were selected as candidates for accommodating heat recovery from nearby waste treatment stations, where a massive amount of biogas can be produced on a regular basis. The scenario involves constructing cogeneration systems in each waste treatment station and producing electricity and heat. The amounts of biogas production for each station are estimated based on the monthly treatment capacities surveyed over the most recent years. Heat produced by the cogeneration system is first consumed on site by the waste treatment system to keep the operating temperature at a proper level. If surplus heat is available, it will be transported to the nearest district heating plant. The year-round operation of the cogeneration system was simulated to estimate the electricity and heat production. We considered cost associated with the installation of the cogeneration system and piping as initial investments. Profits from selling electricity and recovering heat are counted as income, while costs associated with buying biogas are expenses. Simple payback periods of 2-10 years were projected under the current economic conditions of South Korea. We found that most of the proposed scenarios can contribute to both energy savings and environmental protection. PMID:26159562

  6. Chemical and ecotoxicological evaluation of biochar produced from residues of biogas production.

    PubMed

    Stefaniuk, Magdalena; Oleszczuk, Patryk; Bartmiński, Piotr

    2016-11-15

    Analyses were carried out for biochars produced at three temperatures of pyrolysis (400, 600 and 800°C) from solid residue from biogas production (RBP). Separated and non-separated RBP from biogas plants employing different biogas production conditions were pyrolyzed. The contents of heavy metals and polycyclic aromatic hydrocarbons (PAHs) (16 PAH US EPA) were analyzed in biochars. The analyses showed that with an increased pyrolysis temperature, there was an increase in the contents of PAHs and of certain heavy metals (Cr, Cu, Cd, Pb and Mn). In the ecotoxicological tests, it was noted that the effect depended on the temperature of pyrolysis and on the feedstock from which the biochar was produced. The least harmful effect on the test organisms was from biochar produced by separated RBP in a biogas plant operating in mesophilic conditions. The most negative effect on the test organisms was characteristic of biochar produced from non-separated mesophilic RBP. This study shows that the main factors determining the level of toxicity of biochars produced from RBP towards various living organisms are both the method of feedstock production and the temperature at which the process of pyrolysis is conducted. PMID:27450333

  7. Screening of different Trichoderma species against agriculturally important foliar plant pathogens.

    PubMed

    Prabhakaran, Narayanasamy; Prameeladevi, Thokala; Sathiyabama, Muthukrishnan; Kamil, Deeba

    2015-01-01

    Different isolates of Trichoderma were isolated from soil samples which were collected from different part of India. These isolates were grouped into four Trichoderma species viz., Trichoderma asperellum (Ta), T. harzianum (Th), T. pseudokoningii (Tp) and T. longibrachiatum (Tl) based on their morphological characters. Identification of the above isolates was also confirmed through ITS region analysis. These Trichoderma isolates were tested for in vitro biological control of Alternaria solani, Bipolaris oryzae, Pyricularia oryzae and Sclerotinia scierotiorum which cause serious diseases like early blight (target spot) of tomato and potato, brown leaf spot disease in rice, rice blast disease, and white mold disease in different plants. Under in vitro conditions, all the four species of Trichoderma (10 isolates) proved 100% potential inhibition against rice blast pathogen Pyracularia oryzae. T. harzianum (Th-01) and T. asperellum (Ta-10) were effective with 86.6% and 97.7%, growth inhibition of B. oryzae, respectively. Among others, T. pseudokoningii (Tp-08) and T. Iongibrachiatum (Tl-09) species were particularly efficient in inhibiting growth of S. sclerotiorum by 97.8% and 93.3%. T. Iongibrachiatum (TI-06 and TI-07) inhibited maximum mycelial growth of A. solani by 87.6% and 84.75. However, all the T. harzianum isolates showed significantly higher inhibition against S. sclerotiorum (CD value 9.430), causing white mold disease. This study led to the selection of potential Trichoderma isolates against rice blast, early blight, brown leaf spot in rice and white mold disease in different crops. PMID:26536792

  8. Soil-to-plant transfer factors for radiocesium and radiostrontium in agricultural systems

    SciTech Connect

    Nisbet, A.F.; Woodman, R.F.M.

    2000-03-01

    A database of soil-to-plant transfer factors for radiocesium and radiostrontium has been compiled for arable crops from published and unpublished sources. The database is more extensive than previous compilations of data published by the International Union of Radioecologists, containing new information for Scandinavia and Greece in particular. It also contains ancillary data on important soil characteristics. The database is sub-divided into 28 soil-crop combinations, covering four soil types and seven crop groups. Statistical analyses showed that transfer factors for radiocesium could not generally be predicted as a function of climatic region, type of experiment, age of contamination, or silt characteristics. However, significant relationships accounting for more than 30% of the variability in transfer factor were identified between transfer factors for radiostrontium and soil pH/organic matter status for a few soil-crop combinations. Best estimate transfer factors for radiocesium and radiostrontium were calculated for 28 soil-crop combinations, based on their geometric means: only the edible parts were considered. To predict the likely value of future individual transfer factors, 95% confidence intervals were also derived. A comparison of best estimate transfer factors derived in this study with recommended values published by the International Union of Radioecologists in 1989 and 1992 was made for comparable soil-crop groupings. While there were no significant differences between the best estimate values derived in this study and the 1992 data, radiological assessments that still use 1989 data may be unnecessarily cautious.

  9. Addressing the complexity and diversity of agricultural plant volatiles: a call for the integration of laboratory- and field-based analyses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    As the sophistication and sensitivity of chemical instrumentation increases so do the number of applications. Correspondingly, new questions and opportunities for systems previously studied also arise. As with most plants, the emission of volatiles from agricultural products is complex and varies am...

  10. Local-scale spatial modelling for interpolating climatic temperature variables to predict agricultural plant suitability

    NASA Astrophysics Data System (ADS)

    Webb, Mathew A.; Hall, Andrew; Kidd, Darren; Minansy, Budiman

    2016-05-01

    Assessment of local spatial climatic variability is important in the planning of planting locations for horticultural crops. This study investigated three regression-based calibration methods (i.e. traditional versus two optimized methods) to relate short-term 12-month data series from 170 temperature loggers and 4 weather station sites with data series from nearby long-term Australian Bureau of Meteorology climate stations. The techniques trialled to interpolate climatic temperature variables, such as frost risk, growing degree days (GDDs) and chill hours, were regression kriging (RK), regression trees (RTs) and random forests (RFs). All three calibration methods produced accurate results, with the RK-based calibration method delivering the most accurate validation measures: coefficients of determination ( R 2) of 0.92, 0.97 and 0.95 and root-mean-square errors of 1.30, 0.80 and 1.31 °C, for daily minimum, daily maximum and hourly temperatures, respectively. Compared with the traditional method of calibration using direct linear regression between short-term and long-term stations, the RK-based calibration method improved R 2 and reduced root-mean-square error (RMSE) by at least 5 % and 0.47 °C for daily minimum temperature, 1 % and 0.23 °C for daily maximum temperature and 3 % and 0.33 °C for hourly temperature. Spatial modelling indicated insignificant differences between the interpolation methods, with the RK technique tending to be the slightly better method due to the high degree of spatial autocorrelation between logger sites.

  11. Plant physiological models of heat, water and photoinhibition stress for climate change modelling and agricultural prediction

    NASA Astrophysics Data System (ADS)

    Nicolas, B.; Gilbert, M. E.; Paw U, K. T.

    2015-12-01

    Soil-Vegetation-Atmosphere Transfer (SVAT) models are based upon well understood steady state photosynthetic physiology - the Farquhar-von Caemmerer-Berry model (FvCB). However, representations of physiological stress and damage have not been successfully integrated into SVAT models. Generally, it has been assumed that plants will strive to conserve water at higher temperatures by reducing stomatal conductance or adjusting osmotic balance, until potentially damaging temperatures and the need for evaporative cooling become more important than water conservation. A key point is that damage is the result of combined stresses: drought leads to stomatal closure, less evaporative cooling, high leaf temperature, less photosynthetic dissipation of absorbed energy, all coupled with high light (photosynthetic photon flux density; PPFD). This leads to excess absorbed energy by Photosystem II (PSII) and results in photoinhibition and damage, neither are included in SVAT models. Current representations of photoinhibition are treated as a function of PPFD, not as a function of constrained photosynthesis under heat or water. Thus, it seems unlikely that current models can predict responses of vegetation to climate variability and change. We propose a dynamic model of damage to Rubisco and RuBP-regeneration that accounts, mechanistically, for the interactions between high temperature, light, and constrained photosynthesis under drought. Further, these predictions are illustrated by key experiments allowing model validation. We also integrated this new framework within the Advanced Canopy-Atmosphere-Soil Algorithm (ACASA). Preliminary results show that our approach can be used to predict reasonable photosynthetic dynamics. For instances, a leaf undergoing one day of drought stress will quickly decrease its maximum quantum yield of PSII (Fv/Fm), but it won't recover to unstressed levels for several days. Consequently, cumulative effect of photoinhibition on photosynthesis can cause

  12. LCA as a decision support tool for the environmental improvement of the operation of a municipal wastewater treatment plant.

    PubMed

    Pasqualino, Jorgelina C; Meneses, Montse; Abella, Montserrat; Castells, Francesc

    2009-05-01

    Life cycle assessment (LCA) methodology is used to evaluate the environmental profile of a product or process from its origin to its final destination. In this paper we used LCA to evaluate the current situation of a wastewater treatment plant and identify improvement alternatives. Currently, the highest environmental impacts are caused by the stages of the plant with the highest energy consumption, the use of biogas from anaerobic digestion (95% burned in torch) and the final destination of the sludge (98.6% for agricultural use and 1.4% for compost). We propose four alternatives for biogas applications and five alternatives for sludge applications and compare them to the current situation. The alternatives were incorporated in a decision support system to identify and prioritize the most positive environmental option. Using biogas to produce electricity or a combination of electricity and heat provided the best environmental options since the energy produced would be enough to supply all the stages of the plant, thus reducing their environmental impact. The best environmental option for the final destination of the sludge is to combine the current situation (fertilizer replacement) with use of the sludge in a cement plant (as a replacement for fuel and raw material). PMID:19534150

  13. [The change in efficiency of protective measures for reduction of 137Cs accumulation by agricultural plants in various periods after the Chernobyl accident].

    PubMed

    Panov, A V; Aleksakhin, R M; Muzalevskaia, A A

    2011-01-01

    Dynamics of 137Cs transfer factors to plants and the effects of protective measures on this radionuclide accumulation in the agricultural production is estimated on the example of the south-western regions of Bryansk District. Three periods in decreasing the 137Cs content in plants during 20 years after the Chernobyl accident are identified. The contribution of radionuclide decay, natural biogeochemical processes and protective measures aimed at reduction of the 137Cs accumulation in agricultural plants during various periods after radioactive fallout is shown. Maximum permissible levels of 137Cs contamination of cultivated lands, where crop products meeting current standards may be obtained, at different scopes of protective measures on radioactive-contaminated territories are forecasted. Periods after radioactive fallout, when crop and forage products meeting radiological standards are obtained, are assessed. PMID:21520624

  14. NEEM: UNUSUALLY VERSATILE PLANT GENUS AZADIRACHTA WITH MANY USEFUL AND SO FAR INSUFFICIENTLY EXPLOITED PROPERTIES FOR AGRICULTURE, MEDICINE, AND INDUSTRY.

    PubMed

    Hummel, H E; Langner, S S; Leithold, G; Schmutterer, H

    2014-01-01

    Neem plants (Rutales: Meliaceae) are well known for their multitude of human benefits in various fields. Specifically well investigated are the Indian neem tree Azadirachta indica A. Juss., the Thai neem A. siamensis Val., the originally Malaysian/Philippinean neem A. excelsa (Jack) and, as a close relative, the Persian lilac, Melia azedarach. The major and most active natural products are azadirachtin, salannin, nimbin and marrangin from Azadirachta species, and azadirachtin analogues like meliantriol from Melia species. Neem fruits, leaves, bark, and roots have specific virtues. They have been traditionally exploited for a considerable part of human history and are documented in Sanskrit texts. Due to human activity in trade and travel both at land and sea, the plant species has been distributed around the globe and is cultivated in many tropical, and subtropical regions. A multitude of natural products of neem have been isolated, chemically characterized or identified, and investigated for their properties in the management of insects, Acarina, Crustacea, nematodes, bacteria, fungi, viruses and soil fertility (for reviews see Kraus, 2002; Schmutterer, 2002A; Rembold, 2002; Koul, 2004; Schmutterer and Huber, 2005; Kleeberg and Strang, 2009; Hummel et al., 2008, 2011, 2012). Neem products are virtually nontoxic, compatible with beneficial insects, pollinators and bees. They are environmentally benign, sustainable, renewable, and of a price affordable for developed countries. In conclusion, neem is a prime example of a natural resource with many beneficial applications in agriculture, human and veterinary medicine. So far, its use is practically free of resistance problems which are frustratingly prevalent in many areas of synthetic insecticide and drug development. Investigating more neem applications will increase future human welfare and health while being of general ecological benefit to the planet. PMID:26084100

  15. Ultrasound assisted biogas production from landfill leachate

    SciTech Connect

    Oz, Nilgün Ayman Yarimtepe, Canan Can

    2014-07-15

    Highlights: • Effect of low frequency ultrasound pretreatment on leachate was investigated. • Three different ultrasound energy inputs (200, 400 and 600 W/l) was applied. • Low-frequency ultrasound treatment increased soluble COD in landfill leachate. • Application of ultrasound to leachate increased biogas production about 40%. • Application of ultrasound to leachate increased total methane production rate about 20%. - Abstract: The aim of this study is to increase biogas production and methane yield from landfill leachate in anaerobic batch reactors by using low frequency ultrasound as a pretreatment step. In the first part of the study, optimum conditions for solubilization of organic matter in leachate samples were investigated using various sonication durations at an ultrasound frequency of 20 kHz. The level of organic matter solubilization during ultrasonic pretreatment experiments was determined by calculating the ratio of soluble chemical oxygen demand (sCOD) to total chemical oxygen demand (tCOD). The sCOD/tCOD ratio was increased from 47% in raw leachate to 63% after 45 min sonication at 600 W/l. Non-parametric Friedman’s test indicated that ultrasonic pretreatment has a significant effect on sCOD parameter for leachate (p < 0.05). In the second part of the study, anaerobic batch reactors were operated for both ultrasonically pretreated and untreated landfill leachate samples in order to assess the effect of sonication on biogas and methane production rate. In anaerobic batch reactor feed with ultrasonically pretreated leachate, 40% more biogas was obtained compared to the control reactor. For statistical analysis, Mann–Whitney U test was performed to compare biogas and methane production rates for raw and pretreated leachate samples and it has been found that ultrasonic pretreatment significantly enhanced biogas and methane production rates from leachate (p < 0.05) in anaerobic batch reactors. The overall results showed that low frequency

  16. Multiwalled carbon nanotubes and c60 fullerenes differentially impact the accumulation of weathered pesticides in four agricultural plants.

    PubMed

    De La Torre-Roche, Roberto; Hawthorne, Joseph; Deng, Yingqing; Xing, Baoshan; Cai, Wenjun; Newman, Lee A; Wang, Qiang; Ma, Xingmao; Hamdi, Helmi; White, Jason C

    2013-01-01

    The effect of multiwalled carbon nanotubes (MWCNT) or C60 fullerenes on the uptake of weathered chlordane or DDx (DDT + metabolites) by Cucurbita pepo (zucchini), Zea mays (corn), Solanum lycopersicum (tomato), and Glycine max (soybean) was investigated. The plants were grown in 50 g of soil with weathered chlordane (2150 ng/g) and DDx (118 ng/g) that was amended with 0, 500, 1000, or 5000 mg/kg MWCNT or C60. After 28 d, the root and shoot content of chlordane components and DDx was determined by GC-MS. Zucchini and tomato growth were unaffected by carbon nanomaterial coexposure, although C60 at 500 mg/kg reduced corn and soybean biomass by 36.5-45.0%. Total chlordane content ranged from 1490 (tomato) to 4780 (zucchini) ng; DDx amounts ranged from 77.8 (corn) to 395 ng (zucchini). MWCNT coexposure decreased chlordane and DDx accumulation 21-80% across all crops, depending on species and nanotube concentration. Conversely, C60 had species- and contaminant-specific effects on pesticide uptake, ranging from complete suppression of DDx uptake (corn/tomato) to 34.9% increases in chlordane accumulation (tomato/soybean). The data show that pesticide accumulation varies greatly with crop species and carbon nanomaterial type/concentration. These findings have implications for food safety and for the use of engineered nanomaterials in agriculture. PMID:24079803

  17. Tracking Movement of Plant Carbon Through Soil to Water by Lignin Phenol Stable Carbon Isotope Composition in a Small Agricultural Watershed

    NASA Astrophysics Data System (ADS)

    Crooker, K.; Filley, T.; Six, J.; Frey, J.

    2005-12-01

    Few studies integrate land cover, soil physical structure, and aquatic physical fractions when investigating the fate of agricultural carbon in watersheds. In crop systems that involve rotations of soy (a C3 plant) and corn (a C4 plant) the large intrinsic differences in stable carbon isotope values and lignin plus cutin chemistry enable tracking of plant carbon movement from soil fractions to DOM and overland flow during precipitation events. In a small (~3Km2) agricultural basin in central Indiana, we studied plant carbon dynamics in a soy/corn agricultural rotation (2004-2005) to determine the relative inputs of these two plants to soil fractions and the resultant contributions to dissolved, colloidal, and particulate organic matter when mobilized. Using bulk isotope values the fraction of carbon derived from corn in macroaggregates (>250 micron), microaggregates (53-250 mm), and silts plus clays (<53 mm) ranged from 39, 49, to 42%, respectively. Unlike bulk analyses, compound specific isotope analysis of lignin in the soil fractions revealed a wide range of relative inputs among the monomers with cinnamyl phenols being almost exclusively (~ 93%) derived from corn. Syringyl phenols ranged from 75-56% corn and vanillyl phenols ranged from 37-40% corn carbon. The relative input among the fractions mirrors closely the comparative plant chemistry abundances between soy and corn. During export of DOM from the land to the stream the relative abundance of plant source varied with discharge (0.05-1.8 m3/sec) as increases in flow increased the relative export of corn-derived C from the fields. Over the full range of flows lignin phenols varied from 0.05 to 82% corn-derived with the greatest relative corn input for cinnamyl and syringyl carbon. The trend with stream discharge indicates a progressive movement of particulate corn residues with overland flow. Ongoing studies look to resolve contributions of algae, bacteria and terrestrial plants to soil fractions and their

  18. Disintegration in the biogas sector--technologies and effects.

    PubMed

    Schumacher, Britt; Wedwitschka, Harald; Hofmann, Josephine; Denysenko, Velina; Lorenz, Helge; Liebetrau, Jan

    2014-09-01

    Pretreatment of organic material prior to anaerobic digestion is seen as an option to increase the overall efficiency of the process. An overview of physical, chemical, and biological disintegration (DT) of substrates in the biogas sector is given. The energy demands DT were surveyed. The technologies were evaluated by reference to the Technology Readiness Assessment Guide of the U.S. Department of Energy. The evaluation focuses on ligno-cellulosic substrates like straw. Data of a survey among biogas plant operators were analyzed regarding the prevalence of disintegration technology classes in Germany. Furthermore, biochemical methane potential tests were conducted in laboratory scale to determine the specific methane yields of un-/treated barley straw (thermal pressure hydrolysis (TPH)). A methane potential of 228 ml CH4/g VS was measured for untreated barley straw; and of 251 ml CH4/g VS for TPH-straw (190 °C, 30 min). The reaction rates in BMP were calculated between 0.0976 and 0.1443 d(-1). PMID:24589495

  19. Fuel Cells on Bio-Gas (Presentation)

    SciTech Connect

    Remick, R. J.

    2009-03-04

    The conclusions of this presentation are: (1) Fuel cells operating on bio-gas offer a pathway to renewable electricity generation; (2) With federal incentives of $3,500/kW or 30% of the project costs, reasonable payback periods of less than five years can be achieved; (3) Tri-generation of electricity, heat, and hydrogen offers an alternative route to solving the H{sub 2} infrastructure problem facing fuel cell vehicle deployment; and (4) DOE will be promoting bio-gas fuel cells in the future under its Market Transformation Programs.

  20. Effect of two biogas residues' application on copper and zinc fractionation and release in different soils.

    PubMed

    Chen, Miao; Cui, Yanshan; Bai, Fan; Wang, Jiaojiao

    2013-09-01

    Biogas residue (BR) is widely used as a new green fertilizer in agriculture in China. However, it often contains a high concentration of heavy metals so its application should cause our concern. An incubation experiment was conducted to study the risk of pig biogas residue (PBR) and chicken biogas residue (CBR) application on Liuminying soil (LS) and Yixing soil (YS). The soils were incubated for one, three and six months with 0, 2%, 4% and 6% addition of BRs. According to BCR extraction results, the PBR and CBR applications induced an increase in the concentration of exchangeable fraction of Zn. As for the concentration of exchangeable fraction of Cu, an increase was only observed in the treatments with PBR application. The heavy metal binding intensity also showed a similar trend. With the PBR application, for the LS and YS, the highest concentrations of exchangeable Zn increased 3.6 and 9.5 times, respectively, while the exchangeable Cu was increased by 52.6% and 187.1%. Dissolved organic carbon was the limiting factor for the exchangeable Cu while the exchangeable Zn was controlled by soil pH. PBR presented more agricultural risk than CBR when used as fertilizer. Meanwhile, BRs were more adaptable to LS than YS according to the heavy metal release results. PMID:24520730

  1. Process for electric power production using a biogas

    SciTech Connect

    Archer, D.H.; Bauer, F.I.; Vidt, E.J.

    1987-01-27

    A process is described for the production of electric power with a biogas used as a fuel for an electric power producing combustion turbine which drives a generator. The turbine will accept such a biogas only at a temperature below a predetermined temperature, wherein a biomass is gasified to produce a hot stream of a biogas. The biogas is at temperatures of between about 650/sup 0/-875/sup 0/C and contains vaporized tar components and solid particulate matter. It is characterized in that: the hot stream of biogas, consisting essentially of a biogas, vaporized tars and solid particulate matter, has water injected thereto partially cool the biogas to a temperature below the predetermined temperature by vaporization of the water. However, the biogas is above a temperature at which the vaporized tars in the biogas would condense out of the stream; filtering the partially cooled biogas to remove the particulate matter; and directly charging the partially cooled, filtered biogas containing the vaporized water and vaporized tars to an electric power producing combustion turbine to produce electric power.

  2. Connecticut Vocational Agriculture Curriculum.

    ERIC Educational Resources Information Center

    Connecticut State Dept. of Education, Hartford. Bureau of Vocational Services.

    Designed for use in the Connecticut Regional Vocational Agriculture Centers, this curriculum provides exploratory and specialization units for four major areas of agriculture. These are Agriculture Mechanics, Animal Science, Natural Resources, and Plant Science. The exploratory units are required for grades 9 and 10, while the specialization units…

  3. Science of Agricultural Plants

    ERIC Educational Resources Information Center

    Murdock, Ashleigh Barbee, Ed.

    2010-01-01

    Secondary vocational-technical education programs in Mississippi are faced with many challenges resulting from sweeping educational reforms at the national and state levels. Schools and teachers are increasingly being held accountable for providing true learning activities to every student in the classroom. This accountability is measured through…

  4. Bio-gas production from alligator weeds

    NASA Technical Reports Server (NTRS)

    Latif, A.

    1976-01-01

    Laboratory experiments were conducted to study the effect of temperature, sample preparation, reducing agents, light intensity and pH of the media, on bio-gas and methane production from the microbial anaerobic decomposition of alligator weeds (Alternanthera philoxeroides. Efforts were also made for the isolation and characterization of the methanogenic bacteria.

  5. Biogas desulfurization using autotrophic denitrification process.

    PubMed

    Bayrakdar, Alper; Tilahun, Ebrahim; Calli, Baris

    2016-01-01

    The aim of this study was to evaluate the performance of an autotrophic denitrification process for desulfurization of biogas produced from a chicken manure digester. A laboratory scale upflow fixed bed reactor (UFBR) was operated for 105 days and fed with sodium sulfide or H2S scrubbed from the biogas and nitrate as electron donor and acceptor, respectively. The S/N ratio (2.5 mol/mol) of the feed solution was kept constant throughout the study. When the UFBR was fed with sodium sulfide solution with an influent pH of 7.7, about 95 % sulfide and 90 % nitrate removal efficiencies were achieved. However, the inlet of the UFBR was clogged several times due to the accumulation of biologically produced elemental sulfur particles and the clogging resulted in operational problems. When the UFBR was fed with the H2S absorbed from the biogas and operated with an influent pH of 8-9, around 98 % sulfide and 97 % nitrate removal efficiencies were obtained. In this way, above 95 % of the H2S in the biogas was removed as elemental sulfur and the reactor effluent was reused as scrubbing liquid without any clogging problem. PMID:26428238

  6. Utilization and recycle of agricultural wastes and residues

    SciTech Connect

    Shuler, M.L.

    1980-01-01

    A critical examination of proposed schemes for treating agricultural wastes and residues as sources of both food and energy (biogas, for instance) explores three questions as they relate to the various groups of biological and thermochemical conversion processes: What is the state of the art, what are the critical R and D needs, and which methods have the most commercial potential. The problem of cellulose degradation represents a link common to all types of agricultural waste- and residue-conversion schemes; depending on the process objectives, cellulose can be perceived as either a valuable resource or a stubborn impediment. The subjects addressed include (1) the possible methods of directly converting animal manure and plant residues into animal feedstuffs or human food, (2) the potential for anaerobic fermentation processes (particularly farm-size units) to produce energy and food from agricultural wastes while effectively controlling pollution, and (3) the thermochemical conversion of residues via combustion, partial oxidation-pyrolysis, and fluid-phase reactors. A discussion of the application of all these techniques to a single waste (rye-grass straw) places the array of available options into perspective.

  7. Membrane separation of CO{sub 2} and H{sub 2}S from biogas for industrial cogeneration purposes

    SciTech Connect

    Calabro, L.A.; Friedman, A.A.; Stern, S.A.; Krishnakumar, B.

    1996-11-01

    Membrane separation processes are being used on an increasingly larger scale for a wide variety of gas mixtures. The objective of this study was to assess the feasibility and economics of a new membrane separation process for upgrading the CH{sub 4} content of biogas produced by anaerobic processes in industrial wastewater treatment plants. The upgraded biogas can be utilized for the cogeneration of electricity and heat, which could result in substantial savings in plant operation costs. This paper reports preliminary results of laboratory and pilot plant studies on the separation of CO{sub 2} and H{sub 2}S from mixtures with CH{sub 4} by means of polymer membranes in the form of hollow fibers. Both synthetic CH{sub 4}/CO{sub 2} and CH{sub 4}/CO{sub 2}H{sub 2}S mixtures and biogas produced in a municipal wastewater treatment plant were used in a range of elevated pressures selected to determine optimum operating conditions. These data were used to develop economic assessments of cogeneration systems fueled with biogas upgraded by membrane systems.

  8. The potential for tree planting strategies to reduce local and regional ecosystem impacts of agricultural ammonia emissions.

    PubMed

    Bealey, W J; Dore, A J; Dragosits, U; Reis, S; Reay, D S; Sutton, M A

    2016-01-01

    Trees are very effective at capturing both gaseous and particulate pollutants from the atmosphere. But while studies have often focussed on PM and NOx in the urban environment, little research has been carried out on the tree effect of capturing gaseous emissions of ammonia in the rural landscape. To examine the removal or scavenging of ammonia by trees a long-range atmospheric model (FRAME) was used to compare two strategies that could be used in emission reduction policies anywhere in the world where nitrogen pollution from agriculture is a problem. One strategy was to reduce the emission source strength of livestock management systems by implementing two 'tree-capture' systems scenarios - tree belts downwind of housing and managing livestock under trees. This emission reduction can be described as an 'on-farm' emission reduction policy, as ammonia is 'stopped' from dispersion outside the farm boundaries. The second strategy was to apply an afforestation policy targeting areas of high ammonia emission through two planting scenarios of increasing afforestation by 25% and 50%. Both strategies use trees with the aim of intercepting NH3 emissions to protect semi-natural areas. Scenarios for on-farm emission reductions showed national reductions in nitrogen deposition to semi-natural areas of 0.14% (0.2 kt N-NHx) to 2.2% (3.15 kt N-NHx). Scenarios mitigating emissions from cattle and pig housing gave the highest reductions. The afforestation strategy showed national reductions of 6% (8.4 kt N-NHx) to 11% (15.7 kt N-NHx) for 25% and 50% afforestation scenarios respectively. Increased capture by the planted trees also showed an added benefit of reducing long range effects including a decrease in wet deposition up to 3.7 kt N-NHx (4.6%) and a decrease in export from the UK up to 8.3 kt N-NHx (6.8%). PMID:26413804

  9. Improving the prediction of arsenic contents in agricultural soils by combining the reflectance spectroscopy of soils and rice plants

    NASA Astrophysics Data System (ADS)

    Shi, Tiezhu; Wang, Junjie; Chen, Yiyun; Wu, Guofeng

    2016-10-01

    Visible and near-infrared reflectance spectroscopy provides a beneficial tool for investigating soil heavy metal contamination. This study aimed to investigate mechanisms of soil arsenic prediction using laboratory based soil and leaf spectra, compare the prediction of arsenic content using soil spectra with that using rice plant spectra, and determine whether the combination of both could improve the prediction of soil arsenic content. A total of 100 samples were collected and the reflectance spectra of soils and rice plants were measured using a FieldSpec3 portable spectroradiometer (350-2500 nm). After eliminating spectral outliers, the reflectance spectra were divided into calibration (n = 62) and validation (n = 32) data sets using the Kennard-Stone algorithm. Genetic algorithm (GA) was used to select useful spectral variables for soil arsenic prediction. Thereafter, the GA-selected spectral variables of the soil and leaf spectra were individually and jointly employed to calibrate the partial least squares regression (PLSR) models using the calibration data set. The regression models were validated and compared using independent validation data set. Furthermore, the correlation coefficients of soil arsenic against soil organic matter, leaf arsenic and leaf chlorophyll were calculated, and the important wavelengths for PLSR modeling were extracted. Results showed that arsenic prediction using the leaf spectra (coefficient of determination in validation, Rv2 = 0.54; root mean square error in validation, RMSEv = 12.99 mg kg-1; and residual prediction deviation in validation, RPDv = 1.35) was slightly better than using the soil spectra (Rv2 = 0.42, RMSEv = 13.35 mg kg-1, and RPDv = 1.31). However, results also showed that the combinational use of soil and leaf spectra resulted in higher arsenic prediction (Rv2 = 0.63, RMSEv = 11.94 mg kg-1, RPDv = 1.47) compared with either soil or leaf spectra alone. Soil spectral bands near 480, 600, 670, 810, 1980, 2050 and

  10. Regional gene flow and population structure of the wind-dispersed plant species Hypochaeris radicata (Asteraceae) in an agricultural landscape.

    PubMed

    Mix, C; Arens, P F P; Rengelink, R; Smulders, M J M; Van Groenendael, J M; Ouborg, N J

    2006-06-01

    Using microsatellites, we investigated population structure and gene flow of the short-lived, wind-dispersed plant species Hypochaeris radicata in a fragmented agricultural landscape where more than 99% of the nutrient-poor grasslands have disappeared over the last century. We sampled populations in the few remaining high density populations in conservation areas, as well as individuals that occurred, with lower densities, in linear landscape elements, at two spatial scales. In a re-inventory of the landscape, after 3 years, both extinctions and colonizations of populations were observed. Contrary to expectations, no differences in genetic diversity between high and low density populations were observed. Both types of populations had relatively high levels of diversity. Overall genetic differentiation (theta) was 0.04 and significantly different from zero (P < 0.01). A significant isolation-by-distance pattern was found when all populations were simultaneously analysed (r = 0.24, P = 0.013). Isolation by distance was (marginally) significant at the small scale (r = 0.32, P = 0.06), whereas nonsignificant at the large spatial scale (r = -0.05, P = 0.66). A maximization-of-explained-variance procedure resulted in a threshold distance of 3.5 km above which populations were effectively genetically isolated. An additional partial exclusion Bayesian-based assignment test showed that overall 32.3% of the individuals were assigned to their population of origin, 48% were assigned to another population in the area and 19.7% were not assigned. Together, these results suggest high levels of gene flow. Seed dispersal contributes to the observed gene flow up to several hundred metres, which is higher than previously modelled using aerodynamic models on seed dispersal of H. radicata. We discuss the consequences of these results for an evaluation of the probability of persistence of this species in the fragmented landscape. PMID:16689895

  11. Biogas Production from Protein-Rich Biomass: Fed-Batch Anaerobic Fermentation of Casein and of Pig Blood and Associated Changes in Microbial Community Composition

    PubMed Central

    Kovács, Etelka; Wirth, Roland; Maróti, Gergely; Bagi, Zoltán; Rákhely, Gábor; Kovács, Kornél L.

    2013-01-01

    It is generally accepted as a fact in the biogas technology that protein-rich biomass substrates should be avoided due to inevitable process inhibition. Substrate compositions with a low C/N ratio are considered difficult to handle and may lead to process failure, though protein-rich industrial waste products have outstanding biogas generation potential. This common belief has been challenged by using protein-rich substrates, i.e. casein and precipitated pig blood protein in laboratory scale continuously stirred mesophilic fed-batch biogas fermenters. Both substrates proved suitable for sustained biogas production (0.447 L CH4/g protein oDM, i.e. organic total solids) in high yield without any additives, following a period of adaptation of the microbial community. The apparent key limiting factors in the anaerobic degradation of these proteinaceous materials were the accumulation of ammonia and hydrogen sulfide. Changes in time in the composition of the microbiological community were determined by next-generation sequencing-based metagenomic analyses. Characteristic rearrangements of the biogas-producing community upon protein feeding and specific differences due to the individual protein substrates were recognized. The results clearly demonstrate that sustained biogas production is readily achievable, provided the system is well-characterized, understood and controlled. Biogas yields (0.45 L CH4/g oDM) significantly exceeding those of the commonly used agricultural substrates (0.25-0.28 L CH4/g oDM) were routinely obtained. The results amply reveal that these high-energy-content waste products can be converted to biogas, a renewable energy carrier with flexible uses that can replace fossil natural gas in its applications. Process control, with appropriate acclimation of the microbial community to the unusual substrate, is necessary. Metagenomic analysis of the microbial community by next-generation sequencing allows a precise determination of the alterations in

  12. Processed vs. Non-Processed Biowastes for Agriculture: Effects of Post-Harvest Tomato Plants and Biochar on Radish Growth, Chlorophyll Content and Protein Production

    PubMed Central

    Mozzetti Monterumici, Chiara; Rosso, Daniele; Montoneri, Enzo; Ginepro, Marco; Baglieri, Andrea; Novotny, Etelvino Henrique; Kwapinski, Witold; Negre, Michèle

    2015-01-01

    The aim of this work was to address the issue of processed vs. non-processed biowastes for agriculture, by comparing materials widely differing for the amount of process energy consumption. Thus, residual post harvest tomato plants (TP), the TP hydrolysates obtained at pH 13 and 60 °C, and two known biochar products obtained by 650 °C pyrolysis were prepared. All products were characterized and used in a cultivation of radish plants. The chemical composition and molecular nature of the materials was investigated by solid state 13C NMR spectrometry, elemental analysis and potentiometric titration. The plants were analysed for growth and content of chlorophyll, carotenoids and soluble proteins. The results show that the TP and the alkaline hydrolysates contain lignin, hemicellulose, protein, peptide and/or amino acids moieties, and several mineral elements. The biochar samples contain also similar mineral elements, but the organic fraction is characterized mainly by fused aromatic rings. All materials had a positive effect on radish growth, mainly on the diameter of roots. The best performances in terms of plant growth were given by miscanthus originated biochar and TP. The most significant effect was the enhancement of soluble protein content in the plants treated with the lowest energy consumption non processed TP. The significance of these findings for agriculture and the environment is discussed. PMID:25906472

  13. Processed vs. non-processed biowastes for agriculture: effects of post-harvest tomato plants and biochar on radish growth, chlorophyll content and protein production.

    PubMed

    Mozzetti Monterumici, Chiara; Rosso, Daniele; Montoneri, Enzo; Ginepro, Marco; Baglieri, Andrea; Novotny, Etelvino Henrique; Kwapinski, Witold; Negre, Michèle

    2015-01-01

    The aim of this work was to address the issue of processed vs. non-processed biowastes for agriculture, by comparing materials widely differing for the amount of process energy consumption. Thus, residual post harvest tomato plants (TP), the TP hydrolysates obtained at pH 13 and 60 °C, and two known biochar products obtained by 650 °C pyrolysis were prepared. All products were characterized and used in a cultivation of radish plants. The chemical composition and molecular nature of the materials was investigated by solid state 13C NMR spectrometry, elemental analysis and potentiometric titration. The plants were analysed for growth and content of chlorophyll, carotenoids and soluble proteins. The results show that the TP and the alkaline hydrolysates contain lignin, hemicellulose, protein, peptide and/or amino acids moieties, and several mineral elements. The biochar samples contain also similar mineral elements, but the organic fraction is characterized mainly by fused aromatic rings. All materials had a positive effect on radish growth, mainly on the diameter of roots. The best performances in terms of plant growth were given by miscanthus originated biochar and TP. The most significant effect was the enhancement of soluble protein content in the plants treated with the lowest energy consumption non processed TP. The significance of these findings for agriculture and the environment is discussed. PMID:25906472

  14. Environmental balance of the UK biogas sector: An evaluation by consequential life cycle assessment.

    PubMed

    Styles, David; Dominguez, Eduardo Mesa; Chadwick, Dave

    2016-08-01

    Anaerobic digestion (AD) is expanding rapidly in the UK. Previous life cycle assessment (LCA) studies have highlighted the sensitivity of environmental outcomes to feedstock type, fugitive emissions, biomethane use, energy conversion efficiency and digestate management. We combined statistics on current and planned AD deployment with operational data from a survey of biogas plant operators to evaluate the environmental balance of the UK biogas sector for the years 2014 and 2017. Consequential LCA was applied to account for all major environmental credits and burdens incurred, including: (i) substitution of composting, incineration, sewer disposal, field decomposition and animal feeding of wastes; (ii) indirect land use change (ILUC) incurred by the cultivation of crops used for biogas production and to compensate for bakery and brewery wastes diverted from animal feed. In 2014, the UK biogas sector reduced greenhouse gas (GHG) emissions by 551-755Gg CO2e excluding ILUC, or 238-755Gg CO2e including ILUC uncertainty. Fossil energy depletion was reduced by 8.9-10.8PJe, but eutrophication and acidification burdens were increased by 1.8-3.4Gg PO4e and 8.1-14.6Gg SO2e, respectively. Food waste and manure feedstocks dominate GHG abatement, largely through substitution of in-vessel composting and manure storage, whilst food waste and crop feedstocks dominate fossil energy credit, primarily through substitution of natural gas power generation. Biogas expansion is projected to increase environmental credits and loadings by a factor of 2.4 by 2017. If all AD bioelectricity replaced coal generation, or if 90% of biomethane replaced transport diesel or grid natural gas, GHG abatement would increase by 131%, 38% and 20%, respectively. Policies to encourage digestion of food waste and manures could maximize GHG abatement, avoiding the risk of carbon leakage associated with use of crops and wastes otherwise used to feed livestock. Covering digestate stores could largely mitigate

  15. Benchmarking Method for Estimation of Biogas Upgrading Schemes

    NASA Astrophysics Data System (ADS)

    Blumberga, D.; Kuplais, Ģ.; Veidenbergs, I.; Dāce, E.

    2009-01-01

    The paper describes a new benchmarking method proposed for estimation of different biogas upgrading schemes. The method has been developed to compare the indicators of alternative biogas purification and upgrading solutions and their threshold values. The chosen indicators cover both economic and ecologic aspects of these solutions, e.g. the prime cost of biogas purification and storage, and the cost efficiency of greenhouse gas emission reduction. The proposed benchmarking method has been tested at "Daibe" - a landfill for solid municipal waste.

  16. Biogas Potential in the United States (Fact Sheet)

    SciTech Connect

    Not Available

    2013-10-01

    Biogas has received increased attention as an alternative energy source in the United States. The factsheet provides information about the biogas (methane) potential from various sources in the country (by county and state) and estimates the power generation and transportation fuels production (renewable natural gas) potential from these biogas sources. It provides valuable information to the industry, academia and policy makers in support of their future decisions.

  17. Agriculture Education. Agriculture Structures.

    ERIC Educational Resources Information Center

    Stuttgart Public Schools, AR.

    This curriculum guide is designed for group instruction of secondary agricultural education students enrolled in one or two semester-long courses in agriculture structures. The guide presents units of study in the following areas: (1) shop safety, (2) identification and general use of hand tools, (3) power tools, (4) carpentry, (5) blueprint…

  18. Pretreatment of Lignocellulosic Wastes to Improve Ethanol and Biogas Production: A Review

    PubMed Central

    Taherzadeh, Mohammad J.; Karimi, Keikhosro

    2008-01-01

    Lignocelluloses are often a major or sometimes the sole components of different waste streams from various industries, forestry, agriculture and municipalities. Hydrolysis of these materials is the first step for either digestion to biogas (methane) or fermentation to ethanol. However, enzymatic hydrolysis of lignocelluloses with no pretreatment is usually not so effective because of high stability of the materials to enzymatic or bacterial attacks. The present work is dedicated to reviewing the methods that have been studied for pretreatment of lignocellulosic wastes for conversion to ethanol or biogas. Effective parameters in pretreatment of lignocelluloses, such as crystallinity, accessible surface area, and protection by lignin and hemicellulose are described first. Then, several pretreatment methods are discussed and their effects on improvement in ethanol and/or biogas production are described. They include milling, irradiation, microwave, steam explosion, ammonia fiber explosion (AFEX), supercritical CO2 and its explosion, alkaline hydrolysis, liquid hot-water pretreatment, organosolv processes, wet oxidation, ozonolysis, dilute-and concentrated-acid hydrolyses, and biological pretreatments. PMID:19325822

  19. Modelling of biogas extraction at an Italian landfill accepting mechanically and biologically treated municipal solid waste.

    PubMed

    Calabrò, Paolo S; Orsi, Sirio; Gentili, Emiliano; Carlo, Meoni

    2011-12-01

    This paper presents the results of the modelling of the biogas extraction in a full-scale Italian landfill by the USEPA LandGEM model and the Andreottola-Cossu approach. The landfill chosen for this research ('Il Fossetto' plant, Monsummano Terme, Italy) had accepted mixed municipal raw waste for about 15 years. In the year 2003 a mechanical biological treatment (MBT) was implemented and starting from the end of the year 2006, the recirculation in the landfill of the concentrated leachate coming from the internal membrane leachate treatment plant was put into practice. The USEPA LandGEM model and the Andreottola-Cossu approach were chosen since they require only input data routinely acquired during landfill management (waste amount and composition) and allow a simplified calibration, therefore they are potentially useful for practical purposes such as landfill gas management. The results given by the models are compared with measured data and analysed in order to verify the impact of MBT on biogas production; moreover, the possible effects of the recirculation of the concentrated leachate are discussed. The results clearly show how both models can adequately fit measured data even after MBT implementation. Model performance was significantly reduced for the period after the beginning of recirculation of concentrated leachate when the probable inhibition of methane production, due to the competition between methanogens and sulfate-reducing bacteria, significantly influenced the biogas production and composition. PMID:21930528

  20. Short-term plant species impact on microbial community structure in soils with long-term agricultural history

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plant-microbial interactions influence a wide range of biogeochemical processes governing the cycling of biologically important elements. Plant species have distinct impacts on biogeochemical processes and plant-microbial interactions in the rhizosphere account for many of the observed differences ...

  1. Biogas from Marine Macroalgae: a New Environmental Technology — Life Cycle Inventory for a Further LCA

    NASA Astrophysics Data System (ADS)

    Romagnoli, Francesco; Blumberga, Dagnija; Gigli, Emanuele

    2010-01-01

    The main goal of this paper is to analyze the innovative process of production of biogas (via fermentation processes) using marine macroalgae as feedstock in a pilot project plant in Augusta (Sicily, Italy). Algae, during their growth, have the capacity to assimilate nutrients and thus subsequent harvesting of the algal biomass recovers the nutrients from biowaste sources giving the possibility to transform negative environmental externalities in positive mainly in terms of eutrophication and climate change impact categories. The paper presents a novel environmental technology for the production of biogas and 2nd generation biofuel (liquid biomethane) after an upgrading process through the use of a cryogenic technology. The paper would also like to make the first attempt at understanding the possibility to implement this innovative technology in the Latvian context. The first calculations and assumptions for the Life Cycle Inventory for a further Life Cycle Assessment are presented.

  2. Activity, life time and effect of hydrolytic enzymes for enhanced biogas production from sludge anaerobic digestion.

    PubMed

    Odnell, Anna; Recktenwald, Michael; Stensén, Katarina; Jonsson, Bengt-Harald; Karlsson, Martin

    2016-10-15

    As an alternative to energy intensive physical methods, enzymatic treatment of sludge produced at wastewater treatment plants for increased hydrolysis and biogas production was investigated. Several hydrolytic enzymes were assessed with a focus on how enzyme activity and life time was influenced by sludge environments. It could be concluded that the activity life time of added enzymes was limited (<24 h) in both waste activated sludge and anaerobic digester sludge environments and that this was, for the majority of enzymes, due to endogenous protease activity. In biogas in situ experiments, subtilisin at a 1% mixture on basis of volatile solids, was the only enzyme providing a significantly increased biomethane production of 37%. However, even at this high concentration, subtilisin could not hydrolyze all available substrate within the life time of the enzyme. Thus, for large scale implementation, enzymes better suited to the sludge environments are needed. PMID:27498254

  3. Effect of organic loading rate and feedstock composition on foaming in manure-based biogas reactors.

    PubMed

    Kougias, P G; Boe, K; Angelidaki, I

    2013-09-01

    Foaming is one of the major problems that occasionally occur in biogas plants, affecting negatively the overall digestion process. In the present study, the effect of organic loading rate (OLR) and feedstock composition on foaming was elucidated in continuous reactor experiments. By stepwise increasing the OLR and the concentration of proteins or lipids in the substrate, foaming in biogas reactors was investigated. No foam formation was observed at the OLR of 3.5 g volatile solids/(L-reactor·day). Organic loading was the main factor affecting foam formation in manure digester, while the organic composition, such as content of proteins or lipids were factors that in combination with the organic loading were triggering foaming. More specifically, gelatine could initiate foam formation at a lower OLR than sodium oleate. Moreover, the volume of foam produced by gelatine was relatively stable and was not increased when further increasing either OLR or gelatine concentration in the feed. PMID:23850819

  4. The potential of residues of furfural and biogas as calcareous soil amendments for corn seed production.

    PubMed

    Zhao, Yunchen; Yan, Zhibin; Qin, Jiahai; Ma, Zhijun; Zhang, Youfu; Zhang, Li

    2016-04-01

    Intensive corn seed production in Northwest of China produced large amounts of furfural residues, which represents higher treatment cost and environmental issue. The broad calcareous soils in the Northwest of China exhibit low organic matter content and high pH, which led to lower fertility and lower productivity. Recycling furfural residues as soil organic and nutrient amendment might be a promising agricultural practice to calcareous soils. A 3-year field study was conducted to evaluate the effects of furfural as a soil amendment on corn seed production on calcareous soil with compared to biogas residues. Soil physical-chemical properties, soil enzyme activities, and soil heavy metal concentrations were assessed in the last year after the last application. Corn yield was determined in each year. Furfural residue amendments significantly decreased soil pH and soil bulk density. Furfural residues combined with commercial fertilizers resulted in the greater cumulative on soil organic matter, total phosphorus, available phosphorus, available potassium, and cation exchange capacity than that of biogas residue. Simultaneously, urease, invertase, catalase, and alkaline phosphatase increased even at the higher furfural application rates. Maize seed yield increased even with lower furfural residue application rates. Furfural residues resulted in lower Zn concentration and higher Cd concentration than that of biogas residues. Amendment of furfural residues led to higher soil electrical conductivity (EC) than that of biogas residues. The addition of furfural residues to maize seed production may be considered to be a good strategy for recycling the waste, converting it into a potential resource as organic amendment in arid and semi-arid calcareous soils, and may help to reduce the use of mineral chemical fertilizers in these soils. However, the impact of its application on soil health needs to be established in long-term basis. PMID:26606935

  5. Optimization of biogas production using MEMS based near infrared inline-sensor

    NASA Astrophysics Data System (ADS)

    Saupe, Ray; Seider, Thomas; Stock, Volker; Kujawski, Olaf; Otto, Thomas; Gessner, Thomas

    2013-03-01

    Due to climate protection and increasing oil prices, renewable energy is becoming extremely important. Anaerobic digestion is a particular environmental and resource-saving way of heat and power production in biogas plants. These plants can be operated decentralized and independent of weather conditions and allow peak load operation. To maximize energy production, plants should be operated at a high efficiency. That means the entire installed power production capacity (e.g. CHP) and biogas production have to be used. However, current plant utilization in many areas is significantly lower, which is economically and environmentally inefficient, since the biochemical process responds to fluctuations in boundary conditions, e.g. mixing in the conditions and substrate composition. At present only a few easily accessible parameters such as fill level, flow rates and temperature are determined on-line. Monitoring of substrate composition occurs only sporadically with the help of laboratory methods. Direct acquisition of substrate composition combined with a smart control and regulation concept enables significant improvement in plant efficiency. This requires a compact, reliable and cost-efficient sensor. It is for this reason that a MEMS sensor system based on NIR spectroscopy has been developed. Requirements are high accuracy, which is the basic condition for exact chemometric evaluation of the sample as well as optimized MEMS design and packaging in order to work in poor environmental conditions. Another issue is sample presentation, which needs an exact adopted optical-mechanical system. In this paper, the development and application of a MEMS-based analyzer for biogas plants will be explained. The above mentioned problems and challenges will be discussed. Measurement results will be shown to demonstrate its performance.

  6. Process flow model of solid oxide fuel cell system supplied with sewage biogas

    NASA Astrophysics Data System (ADS)

    Van herle, J.; Maréchal, F.; Leuenberger, S.; Membrez, Y.; Bucheli, O.; Favrat, D.

    A model for a 100 kW class solid oxide fuel cell (SOFC) system running on biogas from a sewage sludge digestion plant was implemented in a process flow scheme using external steam reforming. The model stack consisted of planar anode supported cells operated at 800 °C displaying state-of-the-art electrochemical performance (0.15 W/cm 2 at 80% fuel utilisation). Real annual data from an existing sewage plant were used as input to the model. From the input of 43 m 3/h biogas (63% CH 4), equivalent to 269 kW (higher heating value, HHV), the SOFC stack was calculated to deliver 131 kW el electricity (48.7%) using a steam-to-carbon ratio of 0.5. This would allow the sewage site to more than cover its own electrical needs, hence to depollute the waste stream at negative energy cost. In its current exploitation using a low efficient gas engine (130 kW), the site is only ≈50% self-sufficient. Special attention was given to the thermal balance of the stack. The stack developed heat (143 kW) could be balanced by endothermal reforming (78 kW) and by cathode excess air λ (=3), allowing a temperature difference between stack inlet and outlet of 200 K. The case was compared to other fuel scenarios. Steam-added biogas behaves basically identically to steam-reformed methane. For partial oxidation of biogas or pure hydrogen feeding, electrical efficiency drops to under 43% while λ needs to be raised to 4.5 to maintain the 200 K thermal gradient over the stack.

  7. Impact assessment of treated/untreated wastewater toxicants discharged by sewage treatment plants on health, agricultural, and environmental quality in the wastewater disposal area.

    PubMed

    Singh, Kunwar P; Mohan, Dinesh; Sinha, Sarita; Dalwani, R

    2004-04-01

    Studies were undertaken to assess the impact of wastewater/sludge disposal (metals and pesticides) from sewage treatment plants (STPs) in Jajmau, Kanpur (5 MLD) and Dinapur, Varanasi (80 MLD), on health, agriculture and environmental quality in the receiving/application areas around Kanpur and Varanasi in Uttar Pradesh, India. The raw, treated and mixed treated urban wastewater samples were collected from the inlet and outlet points of the plants during peak (morning and evening) and non-peak (noon) hours. The impact of the treated wastewater toxicants (metals and pesticides) on the environmental quality of the disposal area was assessed in terms of their levels in different media samples viz., water, soil, crops, vegetation, and food grains. The data generated show elevated levels of metals and pesticides in all the environmental media, suggesting a definite adverse impact on the environmental quality of the disposal area. The critical levels of the heavy metals in the soil for agricultural crops are found to be much higher than those observed in the study areas receiving no effluents. The sludge from the STPs has both positive and negative impacts on agriculture as it is loaded with high levels of toxic heavy metals and pesticides, but also enriched with several useful ingredients such as N, P, and K providing fertilizer values. The sludge studied had cadmium, chromium and nickel levels above tolerable levels as prescribed for agricultural and lands application. Bio-monitoring of the metals and pesticides levels in the human blood and urine of the different population groups under study areas was undertaken. All the different approaches indicated a considerable risk and impact of heavy metals and pesticides on human health in the exposed areas receiving the wastewater from the STPs. PMID:14761695

  8. Utilization of biogas produced by anaerobic digestion of agro-industrial waste: Energy, economic and environmental effects.

    PubMed

    Hublin, Andrea; Schneider, Daniel Rolph; Džodan, Janko

    2014-06-24

    Anaerobic digestion of agro-industrial waste is of significant interest in order to facilitate a sustainable development of energy supply. Using of material and energy potentials of agro-industrial waste, in the framework of technical, economic, and ecological possibilities, contributes in increasing the share of energy generated from renewable energy sources. The paper deals with the benefits arising from the utilization of biogas produced by co-digestion of whey and cow manure. The advantages of this process are the profitability of the plant and the convenience in realizing an anaerobic digestion plant to produce biogas that is enabled by the benefits from the sale of electric energy at favorable prices. Economic aspects are related to the capital cost (€ 2,250,000) of anaerobic digestion treatment in a biogas plant with a 300 kW power and 510 kW heating unit in a medium size farm (450 livestock units). Considering the optimum biogas yield of 20.7 dm(3) kg(-1) of wet substrate and methane content in the biogas obtained of 79%, the anaerobic process results in a daily methane production of 2,500 kg, with the maximum power generation of 2,160,000 kWh y(-1) and heat generation of 2,400,000 kWh y(-1). The net present value (NPV), internal rate of return (IRR) and payback period for implementation of profitable anaerobic digestion process is evaluated. Ecological aspects related to carbon dioxide (CO2) and methane (CH4) emission reduction are assessed. PMID:24963093

  9. REXPO: A catchment model designed to understand and simulate the loss dynamics of plant protection products and biocides from agricultural and urban areas

    NASA Astrophysics Data System (ADS)

    Wittmer, I. K.; Bader, H.-P.; Scheidegger, R.; Stamm, C.

    2016-02-01

    During rain events, biocides and plant protection products are transported from agricultural fields but also from urban sources to surface waters. Originally designed to be biologically active, these compounds may harm organisms in aquatic ecosystems. Although several models allow either urban or agricultural storm events to be predicted, only few combine these two sources, and none of them include biocide losses from building envelopes. This study therefore aims to develop a model designed to predict water and substance flows from urban and agricultural sources to surface waters. We developed a model based on physical principles for water percolation and substance flow including micro- (also called matrix-) and macropore-flows for the agricultural areas together with a model representing sources, sewer systems and a wastewater treatment plant for urban areas. In a second step, the combined model was applied to a catchment where an extensive field study had been conducted. The modelled and measured discharge and compound results corresponded reasonably well in terms of quantity and dynamics. The total cumulative discharge was only slightly lower than the total measured discharge (factor 0.94). The total modelled losses of the agriculturally used herbicide atrazine were slightly lower (∼25%) than the measured losses when the soil pore water distribution coefficient (describing the partition between soil particles and pore water) (Kd) was kept constant and slightly higher if it was increased with time. The modelled urban losses of diuron from facades were within a factor of three with respect to the measured values. The results highlighted the change in importance of the flow components during a rain event from urban sources during the most intensive rain period towards agricultural ones over a prolonged time period. Applications to two other catchments, one neighbouring and one on another continent showed that the model can be applied using site specific data for

  10. Evaluation of Biogas Production Performance and Archaeal Microbial Dynamics of Corn Straw during Anaerobic Co-Digestion with Cattle Manure Liquid.

    PubMed

    Zhang, Benyue; Zhao, Hongyan; Yu, Hairu; Chen, Di; Li, Xue; Wang, Weidong; Piao, Renzhe; Cui, Zongjun

    2016-04-28

    The rational utilization of crop straw as a raw material for natural gas production is of economic significance. In order to increase the efficiency of biogas production from agricultural straw, seasonal restrictions must be overcome. Therefore, the potential for biogas production via anaerobic straw digestion was assessed by exposing fresh, silage, and dry yellow corn straw to cow dung liquid extract as a nitrogen source. The characteristics of anaerobic corn straw digestion were comprehensively evaluated by measuring the pH, gas production, chemical oxygen demand, methane production, and volatile fatty acid content, as well as applying a modified Gompertz model and high-throughput sequencing technology to the resident microbial community. The efficiency of biogas production from fresh straw (433.8 ml/g) was higher than that of production from straw silage and dry yellow straw (46.55 ml/g and 68.75 ml/g, respectively). The cumulative biogas production from fresh straw, silage straw, and dry yellow straw was 365 l(-1) g(-1) VS, 322 l(-1) g-1 VS, and 304 l(-1) g(-1) VS, respectively, whereas cumulative methane production was 1,426.33%, 1,351.35%, and 1,286.14%, respectively, and potential biogas production was 470.06 ml(-1) g(-1) VS, 461.73 ml(-1) g(-1) VS, and 451.76 ml(-1) g(-1) VS, respectively. Microbial community analysis showed that the corn straw was mainly metabolized by acetate-utilizing methanogens, with Methanosaeta as the dominant archaeal community. These findings provide important guidance to the biogas industry and farmers with respect to rational and efficient utilization of crop straw resources as material for biogas production. PMID:26718471

  11. ADJUSTMENT, MAINTENANCE, AND REPAIR OF TILLAGE, PLANTING, SPRAYING, AND FERTILIZING MACHINERY. AGRICULTURAL MACHINERY--SERVICE OCCUPATIONS, MODULE NUMBER 10.

    ERIC Educational Resources Information Center

    Ohio State Univ., Columbus. Center for Vocational and Technical Education.

    THE PURPOSE OF THIS GUIDE IS TO HELP TEACHERS PREPARE POSTSECONDARY-LEVEL STUDENTS FOR THE AGRICULTURAL MACHINERY SERVICE OCCUPATIONS AS PARTS MEN, MECHANICS, MECHANIC'S HELPERS, AND SERVICE SUPERVISORS. IT WAS DESIGNED BY A NATIONAL TASK FORCE ON THE BASIS OF RESEARCH FROM STATE STUDIES. THE MAJOR OBJECTIVE IS TO DEVELOP (1) STUDENT UNDERSTANDING…

  12. DRY NONHEATED ANAEROBIC BIOGAS FERMENTATION USING AGED BEEF CATTLE MANURE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Biogas production at beef cattle feedlots is hard to justify because of the large amounts of dilution water required and the high cost to design and operate conventional water-based digestion systems. Laboratory and field experiments were conducted to determine the feasibility of producing biogas us...

  13. An Introduction to Biogas Production on the Farm.

    ERIC Educational Resources Information Center

    National Center for Appropriate Technology, Butte, MT.

    This three-section report provides introductory information about biogas production and its application to farm environments. The first section discusses the various components of a biogas production system (a system that converts organic wastes into a usable form of energy), explains the system's benefits and liabilities, and provides a brief…

  14. DEVELOPMENT OF AN AFFORDABLE FAMILY-SCALE BIOGAS GENERATOR

    EPA Science Inventory

    From laboratory experiments we calculated that our system would have to deliver 262 liters/hr of biogas to cook a meal. Biogas produced by slurries of various wastes was measured with a two liter bench-top digester system designed by the team. Gas volume was measured by displa...

  15. Crop residue conversion to biogas by dry fermentation

    SciTech Connect

    Jewell, W.J.; Dell'Orto, S.; Fanfoni, K.J.; Fast, S.J.; Jackson, D.A.; Kabrick, R.M.; Gottung, E.J.

    1981-01-01

    A simple 'dry fermentation' process has been developed that may enable economical conversion of drier crop residues to biogas. Results from two years of process definition and scale-up to a 110 m/sup 3/ prototype show that biogas production rates exceeding those necessary to make the dry fermentor competitive have been achieved. 13 refs.

  16. Complexities of nitrogen isotope biogeochemistry in plant-soil systems: implications for the study of ancient agricultural and animal management practices

    PubMed Central

    Szpak, Paul

    2014-01-01

    Nitrogen isotopic studies have the potential to shed light on the structure of ancient ecosystems, agropastoral regimes, and human-environment interactions. Until relatively recently, however, little attention was paid to the complexities of nitrogen transformations in ancient plant-soil systems and their potential impact on plant and animal tissue nitrogen isotopic compositions. This paper discusses the importance of understanding nitrogen dynamics in ancient contexts, and highlights several key areas of archaeology where a more detailed understanding of these processes may enable us to answer some fundamental questions. This paper explores two larger themes that are prominent in archaeological studies using stable nitrogen isotope analysis: (1) agricultural practices (use of animal fertilizers, burning of vegetation or shifting cultivation, and tillage) and (2) animal domestication and husbandry (grazing intensity/stocking rate and the foddering of domestic animals with cultigens). The paucity of plant material in ancient deposits necessitates that these issues are addressed primarily through the isotopic analysis of skeletal material rather than the plants themselves, but the interpretation of these data hinges on a thorough understanding of the underlying biogeochemical processes in plant-soil systems. Building on studies conducted in modern ecosystems and under controlled conditions, these processes are reviewed, and their relevance discussed for ancient contexts. PMID:25002865

  17. A field-scale study of cadmium phytoremediation in a contaminated agricultural soil at Mae Sot District, Tak Province, Thailand: (1) Determination of Cd-hyperaccumulating plants.

    PubMed

    Khaokaew, Saengdao; Landrot, Gautier

    2015-11-01

    The cadmium (Cd) phytoremediation capabilities of Gynura pseudochina, Chromolaena odorata, Conyza sumatrensis, Crassocephalum crepidioides and Nicotiana tabacum were determined by conducting in-situ experiments in a highly Cd-contaminated agricultural field at Mae Sot District, Tak Province, Thailand. Most of these five plant species, which are commonly found in Thailand, previously demonstrated Cd-hyperaccumulating capacities under greenhouse conditions. This study represented an important initial step in determining if any of these plants could, under field-conditions, effectively remove Cd from the Mae Sot contaminated fields, which represent a health threat to thousands of local villagers. All plant species had at least a 95% survival rate on the final harvest day. Additionally, all plant species, except C. odorata, could hyperaccumulate the extractable Cd amounts present in the soil, based on their associated Bioaccumulation Factor (BAF), Translocation Factor (TF), and background Vegetation Factor (VF). Therefore, the four Cd-hyperaccumulating plant species identified in this study may successfully treat a majority of contaminated fields at Mae Sot, as it was previously reported that Cd amounts present in a number of these soils were mostly available. PMID:25454203

  18. Uptake and biodegradation of the antimicrobial sulfadimidine by the species Tripolium pannonicum acting as biofilter and its further biodegradation by anaerobic digestion and concomitant biogas production.

    PubMed

    Turcios, Ariel E; Weichgrebe, Dirk; Papenbrock, Jutta

    2016-11-01

    This project analyses the uptake and biodegradation of the antimicrobial sulfadimidine (SDI) from the culture medium and up to the anaerobic digestion. Tripolium pannonicum was grown under hydroponic conditions with different concentrations of SDI (0, 5 and 10mg·L(-1)) and the fresh biomass, containing different amounts of SDI taken up, was used as substrate for biogas production. SDI was analyzed by liquid chromatography coupled to positive ion electrospray mass spectrometry (ESI LC-MS). Based on the findings, T. pannonicum is able to uptake SDI. The more SDI is in the culture medium, the higher the SDI content in the plant tissue. According to this study, it is possible to produce high yields of biogas using biomass of T. pannonicum containing SDI and at the same time biodegradation of SDI is carried out. The highest specific biogas yield is obtained using shoots as substrate of the plants cultivated at 5mg·L(-1) SDI. PMID:27544919

  19. Rural Industrialization in the Ozarks: Case Study of a New Shirt Plant at Gassville, Arkansas. Agricultural Economic Report No. 123.

    ERIC Educational Resources Information Center

    Jordon, Max F.

    To determine the short term effects of a large apparel plant on the economy of a relatively isolated, highly rural Ozark area (8 counties), characterized by low income and few employment opportunities, this study compared 1959 data on the study area and the state of Arkansas with similar post plant data (1960-1963). Data were compared on income…

  20. Plants for Coastal Dunes of the Gulf and South Atlantic Coasts and Puerto Rico. Agriculture Information Bulletin 460.

    ERIC Educational Resources Information Center

    Craig, Robert M.

    Plants that have been identified as stabilizers and beautifiers of coastal dunes are described in this publication from the Soil Conservation Service (SCS). After years of tests and field trials, the SCS has singled out 43 plants as having good potential for dune revegetation based on their characteristics for erosion control, frequency of…

  1. Comparative study on factors affecting anaerobic digestion of agricultural vegetal residues

    PubMed Central

    2012-01-01

    Background Presently, different studies are conducted related to the topic of biomass potential to generate through anaerobic fermentation process alternative fuels supposed to support the existing fossil fuel resources, which are more and more needed, in quantity, but also in quality of so called green energy. The present study focuses on depicting an optional way of capitalizing agricultural biomass residues using anaerobic fermentation in order to obtain biogas with satisfactory characteristics.. The research is based on wheat bran and a mix of damaged ground grains substrates for biogas production. Results The information and conclusions delivered offer results covering the general characteristics of biomass used , the process parameters with direct impact over the biogas production (temperature regime, pH values) and the daily biogas production for each batch relative to the used material. Conclusions All conclusions are based on processing of monitoring process results , with accent on temperature and pH influence on the daily biogas production for the two batches. The main conclusion underlines the fact that the mixture batch produces a larger quantity of biogas, using approximately the same process conditions and input, in comparison to alone analyzed probes, indicating thus a higher potential for the biogas production than the wheat bran substrate. Adrian Eugen Cioabla, Ioana Ionel, Gabriela-Alina Dumitrel and Francisc Popescu contributed equally to this work PMID:22672892

  2. Influence of biogas flow rate on biomass composition during the optimization of biogas upgrading in microalgal-bacterial processes.

    PubMed

    Serejo, Mayara L; Posadas, Esther; Boncz, Marc A; Blanco, Saúl; García-Encina, Pedro; Muñoz, Raúl

    2015-03-01

    The influence of biogas flow rate (0, 0.3, 0.6, and 1.2 m(3) m(-2) h(-1)) on the elemental and macromolecular composition of the algal-bacterial biomass produced from biogas upgrading in a 180 L photobioreactor interconnected to a 2.5 L external bubbled absorption column was investigated using diluted anaerobically digested vinasse as cultivation medium. The influence of the external liquid recirculation/biogas ratio (0.5 < L/G < 67) on the removal of CO2 and H2S, and on the concentrations of O2 and N2 in the upgraded biogas, was also evaluated. A L/G ratio of 10 was considered optimum to support CO2 and H2S removals of 80% and 100%, respectively, at all biogas flow rates tested. Biomass productivity increased at increasing biogas flow rate, with a maximum of 12 ± 1 g m(-2) d(-1) at 1.2 m(3) m(-2) h(-1), while the C, N, and P biomass content remained constant at 49 ± 2%, 9 ± 0%, and 1 ± 0%, respectively, over the 175 days of experimentation. The high carbohydrate contents (60-76%), inversely correlated to biogas flow rates, would allow the production of ≈100 L of ethanol per 1000 m(3) of biogas upgraded under a biorefinery process approach. PMID:25675110

  3. Performance of photoperiod and light intensity on biogas upgrade and biogas effluent nutrient reduction by the microalgae Chlorella sp.

    PubMed

    Yan, Cheng; Zheng, Zheng

    2013-07-01

    Biogas is an environment-friendly fuel but that must be upgraded before being utilized. The method about removing CO2 from biogas by microalgal culturing using biogas effluent as nutrient medium in this study could effectively upgrade biogas and simultaneously reduce the biogas effluent nutrient. Results showed that the optimum parameters for microalgal growth and biogas effluent nutrient reduction was moderate light intensity with middle photoperiod. While low light intensity with long photoperiod and moderate light intensity with middle photoperiod obtained the best biogas CO2 removal and biogas upgrade effects. Therefore, the optimal parameters were moderate light intensity 350 μmol m(-2)s(-1) with middle photoperiod 14 h light:10h dark. Under this condition, the microalgal dry weight, CH4 concentration, reduction efficiency of chemical oxygen demand, total nitrogen, and total phosphorus was 615.84 ± 33.07 mg L(-1), 92.16 ± 2.83% (v/v), 88.74 ± 3.45%, 83.94 ± 3.51%, and 80.43 ± 4.17%, respectively. PMID:23665690

  4. Rhizoctonia Crown and Root Rot Resistance of Beta Plant Introductions from the USDA, Agricultural Research Service's National Plant Germplasm System, 2010

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Thirty wild beet (Beta vulgaris subsp. maritima (L.) Arcang) plant introduction (PI) accessions from the Beta collection of the USDA-ARS National Plant Germplasm System were screened for resistance to Rhizoctonia root and crown rot, at the USDA-ARS Fort Collins, CO Research Farm. The Rhizoctonia sc...

  5. Plant Pathogen Culture Collections: It Takes a Village to Preserve These Resources Vital to the Advancement of Agricultural Security and Plant Pathology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Plant pathogen culture collections are essential resources in our fight against plant disease and for connecting discoveries of the present with established knowledge of the past. However, available infrastructure in support of culture collections is in serious need of improvement, and we continual...

  6. Inhibition of biogas production by alkyl benzene sulfonates (LAS) in a screening test for anaerobic biodegradability.

    PubMed

    Garcia, M Teresa; Campos, Encarna; Dalmau, Manel; Illán, Patricia; Sánchez-Leal, Joaquin

    2006-02-01

    The effect of the inoculum source on the digestion of linear alkylbenzene sulfonates (LAS) under anaerobic conditions has been investigated. The potential for primary and ultimate LAS biodegradation of anaerobic sludge samples obtained from wastewater treatment plants (WWTPs) of different geographical locations was studied applying a batch test system. It was found that only 4-22% of the LAS added to the batch anaerobic digesters was primarily transformed suggesting a poor primary degradation of the LAS molecule in anaerobic discontinuous systems. Regarding ultimate biodegradation, the addition of LAS to the batch anaerobic digesters caused a reduction on the extent of biogas production. Significant differences in the inhibition extent of the biogas production were observed (4-26%) depending on the sludge used as inoculum. Effect of the surfactant on the anaerobic microorganisms was correlated with its concentration in the aqueous phase. Sorption of LAS on anaerobic sludge affects its toxicity by depletion of the available fraction of the surfactant. LAS content on sludge was related to the total amount of calcium and magnesium extractable ions. The presence of divalent cations promote the association of LAS with anaerobic sludge reducing its bioavailability and the extent of its inhibitory effect on the biogas production. PMID:16453170

  7. Pretreatment of anaerobic digestion effluent with ammonia stripping and biogas purification.

    PubMed

    Lei, Xiaohui; Sugiura, Norio; Feng, Chuanping; Maekawa, Takaaki

    2007-07-16

    In this study, ammonia stripping was optimized for pretreating anaerobic digestion effluent from an anaerobic digestion plant, and the possibility of using CO(2) stripping and biogas injection for adjusting the pH of the effluent before and after the ammonia stripping process was also investigated. For ammonia stripping, the results showed that an overdose of calcium hydroxide, i.e., 27.5g/L wastewater, achieved higher ammonia, phosphorus, chemical oxygen demand, suspended solids, and turbidity removal efficiency. An air flow rate of 5L/min for 1L of wastewater was thought as suitable for engineering application. The pH of the anaerobic digestion effluent can be increased from about 7 to about 9 by CO(2) stripping, however which is insufficient for ammonia stripping. For 1L of wastewater treated after ammonia stripping, the pH can be neutralized to about 7 from greater than 11 through biogas injection at 1L/min for less than 30min, and continuous injection does not decrease the pH. It was roughly estimated that 43m(3) of biogas (CH(4):CO(2) approximately 60%:40%) produced daily could be purified to CH(4):CO(2) approximately 74%:26% by neutralizing the pH of the 5m(3) anaerobic digestion effluent pretreated by ammonia stripping. PMID:17178436

  8. Revival of the biological sunlight-to-biogas energy conversion system.

    PubMed

    De Schamphelaire, Liesje; Verstraete, Willy

    2009-06-01

    In the quest for renewable resources, algae are increasingly receiving attention. Their high growth rate, high CO(2) fixation and their lack of requirement for fertile soil surface represent several advantages as compared to conventional (energy) crops. Through their ability to store large amounts of oils, they qualify as a source for biodiesel. Algal biomass, however, can also be used as such, namely as a substrate for anaerobic digestion. In the present research, we investigated the use of algae for energy generation in a stand-alone, closed-loop system. The system encompasses an algal growth unit for biomass production, an anaerobic digestion unit to convert the biomass to biogas and a microbial fuel cell to polish the effluent of the digester. Nutrients set free during digestion can accordingly be returned to the algal growth unit for a sustained algal growth. Hence, a system is presented that continuously transforms solar energy into energy-rich biogas and electricity. Algal productivities of 24-30 ton VS ha(-1) year(-1) were reached, while 0.5 N m(3) biogas could be produced kg(-1) algal VS. The system described resulted in a power plant with a potential capacity of about 9 kW ha(-1) of solar algal panel, with prospects of 23 kW ha(-1). PMID:19180645

  9. Biogas potential from forbs and grass-clover mixture with the application of near infrared spectroscopy.

    PubMed

    Wahid, Radziah; Ward, Alastair James; Møller, Henrik Bjarne; Søegaard, Karen; Eriksen, Jørgen

    2015-12-01

    This study investigated the potentials of forbs; caraway, chicory, red clover and ribwort plantain as substrates for biogas production. One-, two- and four-cut systems were implemented and the influence on dry matter yields, chemical compositions and methane yields were examined. The two- and four-cut systems resulted in higher dry matter yields (kg [total solid, TS] ha(-1)) compared to the one-cut system. The effect of plant compositions on biogas potentials was not evident. Cumulative methane yields (LCH4kg(-1) [volatile solid, VS]) were varied from 279 to 321 (chicory), 279 to 323 (caraway), 273 to 296 (ribwort plantain), 263 to 328 (red clover) and 320 to 352 (grass-clover mixture), respectively. Methane yield was modelled by modified Gompertz equation for comparison of methane production rate. Near infrared spectroscopy showed potential as a tool for biogas and chemical composition prediction. The best prediction models were obtained for methane yield at 29 days (99 samples), cellulose, acid detergent fibre, neutral detergent fibre and crude protein, (R(2)>0.9). PMID:26386414

  10. Performance assessment of natural gas and biogas fueled molten carbonate fuel cells in carbon capture configuration

    NASA Astrophysics Data System (ADS)

    Barelli, Linda; Bidini, Gianni; Campanari, Stefano; Discepoli, Gabriele; Spinelli, Maurizio

    2016-07-01

    The ability of MCFCs as carbon dioxide concentrator is an alternative solution among the carbon capture and storage (CCS) technologies to reduce the CO2 emission of an existing plant, providing energy instead of implying penalties. Moreover, the fuel flexibility exhibited by MCFCs increases the interest on such a solution. This paper provides the performance characterization of MCFCs operated in CCS configuration and fed with either natural gas or biogas. Experimental results are referred to a base CCS unit constituted by a MCFC stack fed from a reformer and integrated with an oxycombustor. A comparative analysis is carried out to evaluate the effect of fuel composition on energy efficiency and CO2 capture performance. A higher CO2 removal ability is revealed for the natural feeding case, bringing to a significant reduction in MCFC total area (-11.5%) and to an increase in produced net power (+13%). Moreover, the separated CO2 results in 89% (natural gas) and 86.5% (biogas) of the CO2 globally delivered by the CCS base unit. Further investigation will be carried out to provide a comprehensive assessment of the different solutions eco-efficiency considering also the biogas source and availability.

  11. Is sonication effective to improve biogas production and solids reduction in excess sludge digestion?

    PubMed

    Braguglia, C M; Mininni, G; Gianico, A

    2008-01-01

    Results of three semi-continuous anaerobic tests were reported and discussed. Each test was carried out by two parallel anaerobic reactors fed with waste activated sludge, either as it was sampled from the sewage treatment plant of Rome North or previously disintegrated by ultra-sound treatment. Activated sludge was sonicated at the energy input of 5,000 or 2,500 kJ kg(-1) dry solids corresponding to a disintegration degree of approximately 8 or 4%, respectively. Sonication proved to be effective both in increasing VS destruction and cumulative biogas production. The best increase of VS destruction (from 30 to 35%) was achieved in test #3 carried out at high organic load (10 d residence time) and low energy input (2,500 kJ kg(-1) dry solids). The best increase in cumulative biogas production (from 472 to 640 NL after 67 d of tests i.e.) was obtained in test #1 at low organic load (20 d residence time) and high energy input (5,000 kJ kg(-1) dry solids). Specific biogas production varied in the tests carried out with untreated sludge (0.55 - 0.67 Nm3 kg(-1) VS destroyed) but was practically unchanged for all the tests with sonicated sludge (0.7 Nm3 kg(-1) VS destroyed). PMID:18359984

  12. Evaluation of a pilot-scale sewage biogas powered 2.8 kWe Solid Oxide Fuel Cell: Assessment of heat-to-power ratio and influence of oxygen content

    NASA Astrophysics Data System (ADS)

    de Arespacochaga, N.; Valderrama, C.; Peregrina, C.; Mesa, C.; Bouchy, L.; Cortina, J. L.

    2015-12-01

    Biogas from anaerobic digestion of organic matter is a promising renewable energy source and fuel cells appear as a breakthrough technology to improve the performance of the biogas-to-energy valorisation chain. The vast majority of studies addressing biogas energy recovery through Solid Oxide Fuel Cells published in recent years correspond to simulations and lab-scale performance with synthetic biogas. This paper assesses the pilot performance of a 2.8 kWe SOFC unit powered with cleaned sewage biogas for around 700 h in a Wastewater Treatment Plant. The biogas thorough treatment consisting of a biological desulphurisation with a biotrickling filter followed by a deep cleaning step based on adsorption is successful for removing sulphur compounds, siloxanes and hydrocarbons. The influence of the heat-to-power ratio on fuel cell performance is investigated operating the system at O/C ratio of 2, reforming temperature of 550 °C, stack temperature of 800 °C and at a constant voltage of 43 V. At optimized conditions for electrical production satisfying heat demand in the WWTP, system electrical and thermal efficiencies account for 34% and 28%. Cogeneration efficiency remains constant at around 59-62% for all the heat-to-power ratios tested. Furthermore, the impact of the oxygen content in the biogas is also studied.

  13. Process parameters and changes in the microbial community patterns during the first 240 days of an agricultural energy crop digester.

    PubMed

    Weithmann, Nicolas; Weig, Alfons Rupert; Freitag, Ruth

    2016-12-01

    Commercial biogas production takes place by complex microbial communities enclosed in controlled "technical ecosystems". Once established, the communities tend to be resilient towards disturbances, although the relative abundance of their members may vary. The start-up phase, during which the community establishes itself, is therefore decisive for the later performance of the reactor. In this study, we followed the first 240 days of a standard agricultural energy crop digester consisting of a 400 m(3) plug flow fermenter and a 1000 m(3) agitated post digester, operated at 40-45 °C. The feed consisted of corn and later grass silage augmented by ground wheat. Changes in both the eubacterial and methanogenic archaeal communities were followed by automated ribosomal intergenic spacer analysis (ARISA). In addition the copy number of the methyl-coenzyme reductase A (mcrA)-genes found in all known methanogens were followed by quantitative PCR, while selected samples from two phases-one early, one late-of the community structure development were subjected to high throughput sequencing. Biogas volume and composition (CH4, CO2, H2, H2S, O2), pH, ammonia-N, and volatile fatty acids (VFA), were measured as part of the routine process control. VFA/TIC values were calculated on this basis. Whereas the total gas production of the plant established itself at about 2500 m(3) biogas per day within the first months, the composition of the microbial communities showed distinct spatial and temporal differences over the investigated time period. Absolute values for DNA isolation procedures are difficult to certify, hence comparative results on community structures obtained using standardized ARISA with identical primers are of value. Moreover, ARISA patterns can be statistically analyzed to identify distinct subgroups and transitions between them as well as serial correlations. Thereby the microbial community and its structural development can be correlated with statistical

  14. Biogas from sugar beet press pulp as substitute of fossil fuel in sugar beet factories.

    PubMed

    Brooks, L; Parravicini, V; Svardal, K; Kroiss, H; Prendl, L

    2008-01-01

    Sugar beet press pulp (SBP) accumulates as a by-product in sugar factories and it is generally silaged or dried to be used as animal food. Rising energy prices and the opening of the European Union sugar market has put pressure on the manufacturers to find alternatives for energy supply. The aim of this project was to develop a technology in the treatment of SBP that would lead to savings in energy consumption and would provide a more competitive sugar production from sugar beets. These goals were met by the anaerobic digestion of SBP for biogas production. Lab-scale experiments confirmed the suitability of SBP as substrate for anaerobic bacteria. Pilot-scale experiments focused on process optimization and procedures for a quick start up and operational control. Both single-stage and two-stage process configurations showed similar removal efficiency. A stable biogas production could be achieved in single-stage at a maximum volumetric loading rate of 10 kgCSB/(m(3) x d). Degradation efficiency was 75% for VS and 72% for COD. Average specific gas production reached 530 NL/kgCOD(SBP) or 610 NL/kgVS(SBP). (CH(4): 50 to 53%). The first large-scale biogas plant was put into operation during the sugar processing period 2007 at a Hungarian sugar factory. Digesting approximately 50% of the SBP (800 t/d, 22%TS), the biogas produced could substitute about 40% of the natural gas required for the thermal energy supply within the sugar processing. PMID:18957765

  15. Waste ashes for use in agricultural production: I. Liming effect, contents of plant nutrients and chemical characteristics of some metals.

    PubMed

    Zhang, Fu-Shen; Yamasaki, S; Nanzyo, M

    2002-02-01

    The chemical characteristics of 89 municipal waste ashes, including food scrap ash (FSA), animal waste ash (AWA), horticulture waste ash (HWA), sewage sludge ash (SSA) and incinerator bottom ash (IBA), from various locations in Japan were examined with the aim of evaluating their suitability for use in agriculture. Although the waste ashes came from different sources and consisted of various materials, the gross elemental composition was similar. Acid neutralization capacity (liming effect) for the waste ashes was equivalent to 10-30% of CaO and followed the sequence SSA > IBA > AWA > FSA > HWA. Average P concentrations for the five types of waste ashes ranged from 10 to 29 g kg(-1) and average K concentrations ranged from 14 to 63 g kg(-1), respectively. Metal contents in the waste ashes were compared with levels in Japanese agricultural soils. K in the waste ashes was 1.3-6 times higher and Ca was 3-12 times higher; contents of the other metals in FSA, AWA and HWA were generally less than five times higher, but Ni, Cu, Zn, Cd, Sn, Pb in SSA or IBA were approximately 10-200 times higher than those in soils. Moreover, the ceiling amounts of waste ashes that may be applied to main Japanese agricultural soils were calculated by using soil contamination standards for Cu. Water solubility of P and metals in the waste ashes were also examined. PMID:11846166

  16. Source identification of PCDD/Fs in agricultural soils near to a Chinese MSWI plant through isomer-specific data analysis.

    PubMed

    Xu, Meng-Xia; Yan, Jian-Hua; Lu, Sheng-Yong; Li, Xiao-Dong; Chen, Tong; Ni, Ming-Jiang; Dai, Hui-Fen; Cen, Ke-Fa

    2008-04-01

    Isomer-specific data were investigated in order to identify the sources of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in agricultural soils, including Fluvo-aquic and paddy soils, in the vicinity of a Chinese municipal solid waste incineration (MSWI) plant. Homologue and isomer profiles of PCDD/Fs in soils were compared with those of potential sources, including combustion sources, i.e., MSWI flue gas and fly ash; and the impurities in agrochemicals, such as the pentachlorophenol (PCP), sodium pentachlorophenate (PCP-Na) and 1,3,5-trichloro-2-(4-nitrophenoxy) benzene (CNP). The results showed that the PCDD/F isomer profiles of combustion sources and agricultural soils were very similar, especially for PCDFs, although their homologue profiles varied, indicating that all the isomers within each homologue behave identically in the air and soil. Moreover, factor analysis of the isomer compositions among 33 soil samples revealed that the contamination of PCDD/Fs in agricultural soils near the MSWI plant were primarily influenced by the combustion sources, followed by the PCP/PCP-Na and CNP sources. This implication is consistent with our previous findings based on chemometric analysis of homologue profiles of soil and flue gas samples, and identifies PCP/PCP-Na as an additional important source of PCDD/Fs in the local area. This makes the similarities and differences of isomer profiles between Fluvo-aquic and paddy soils more explainable. It is, therefore, advisable to use isomer-specific data for PCDD/F source identifications where possible. PMID:18279911

  17. Facilitated by nature and agriculture: performance of a specialist herbivore improves with host-plant life history evolution, domestication, and breeding.

    PubMed

    Dávila-Flores, Amanda M; DeWitt, Thomas J; Bernal, Julio S

    2013-12-01

    Plant defenses against herbivores are predicted to change as plant lineages diversify, and with domestication and subsequent selection and breeding in the case of crop plants. We addressed whether defense against a specialist herbivore declined coincidently with life history evolution, domestication, and breeding within the grass genus Zea (Poaceae). For this, we assessed performance of corn leafhopper (Dalbulus maidis) following colonization of one of four Zea species containing three successive transitions: the evolutionary transition from perennial to annual life cycle, the agricultural transition from wild annual grass to primitive crop cultivar, and the agronomic transition from primitive to modern crop cultivar. Performance of corn leafhopper was measured through seven variables relevant to development speed, survivorship, fecundity, and body size. The plants included in our study were perennial teosinte (Zea diploperennis), Balsas teosinte (Zea mays parviglumis), a landrace maize (Zea mays mays), and a hybrid maize. Perennial teosinte is a perennial, iteroparous species, and is basal in Zea; Balsas teosinte is an annual species, and the progenitor of maize; the landrace maize is a primitive, genetically diverse cultivar, and is ancestral to the hybrid maize; and, the hybrid maize is a highly inbred, modern cultivar. Performance of corn leafhopper was poorest on perennial teosinte, intermediate on Balsas teosinte and landrace maize, and best on hybrid maize, consistent with our expectation of declining defense from perennial teosinte to hybrid maize. Overall, our results indicated that corn leafhopper performance increased most with the agronomic transition, followed by the life history transition, and least with the domestication transition. PMID:23868032

  18. Cesium and strontium sorption behavior in amended agricultural soils

    NASA Astrophysics Data System (ADS)

    Mehmood, Khalid; Hofmann, Diana; Burauel, Peter; Vereecken, Harry; Berns, Anne E.

    2014-05-01

    Biogas digestates and biochar are emerging soil amendments. Biochar is a byproduct of pyrolysis process which is thermal decomposition of biomass to produce syngas and bio-oil. The use of biochar for soil amendment is being promoted for higher crop yields and carbon sequestration. Currently, the numbers of biogas plants in Germany are increasing to meet the new energy scenarios. The sustainability of biogas industry requires proper disposal options for digestate. Biogas digestates being rich in nutrients are beneficial to enhance agricultural productions. Contrary to the agronomical benefits of these organic amendments, their use can influence the mobility and bioavailability of soil contaminants due to nutrients competition and high organic matter content. So far, the impact of such amendments on highly problematic contaminants like radionuclides is not truly accounted for. In the present study, sorption-desorption behavior of cesium and strontium was investigated in three soils of different origin and texture. Two agricultural soils, a loamy sand and a silty soil, were amended with biochar and digestate in separate experiments, with field application rates of 25 Mg/ha and 34 Mg/ha, respectively. For comparison a third soil, a forest soil, was incubated without any amendment. The amendments were mixed into the top 20 cm of the field soils, resulting in final concentrations of 8-9 g biochar/Kg soil and 11-12 g digestate/Kg soil. The soils were incubated for about six months at room temperature. Sorption-desorption experiments were performed with CsCl and SrCl2 after pre-equilibrating the soils with CaCl2 solutions. The amendments with field application rates did not have a significant effect on the relevant soil parameters responsible for the sorption behavior of the two radionuclides. Comparatively, the soil type lead to distinctive differences in sorption-desorption dynamics of the two radionuclides. Cesium showed a higher affinity for silty soil followed by

  19. Design and scale-up of an oxidative scrubbing process for the selective removal of hydrogen sulfide from biogas.

    PubMed

    Krischan, J; Makaruk, A; Harasek, M

    2012-05-15

    Reliable and selective removal of hydrogen sulfide (H(2)S) is an essential part of the biogas upgrading procedure in order to obtain a marketable and competitive natural gas substitute for flexible utilization. A promising biogas desulfurization technology has to ensure high separation efficiency regardless of process conditions or H(2)S load without the use or production of toxic or ecologically harmful substances. Alkaline oxidative scrubbing is an interesting alternative to existing desulfurization technologies and is investigated in this work. In experiments on a stirred tank reactor and a continuous scrubbing column in laboratory-scale, H(2)S was absorbed from a gas stream containing large amounts of carbon dioxide (CO(2)) into an aqueous solution prepared from sodium hydroxide (NaOH), sodium bicarbonate (NaHCO(3)) and hydrogen peroxide (H(2)O(2)). The influence of pH, redox potential and solution aging on the absorption efficiency and the consumption of chemicals was investigated. Because of the irreversible oxidation reactions of dissolved H(2)S with H(2)O(2), high H(2)S removal efficiencies were achieved while the CO(2) absorption was kept low. At an existing biogas upgrading plant an industrial-scale pilot scrubber was constructed, which efficiently desulfurizes 180m(3)/h of raw biogas with an average removal efficiency of 97%, even at relatively high and strongly fluctuating H(2)S contents in the crude gas. PMID:22440540

  20. N-acetylcysteine in agriculture, a novel use for an old molecule: focus on controlling the plant-pathogen Xylella fastidiosa.

    PubMed

    Muranaka, Lígia S; Giorgiano, Thais E; Takita, Marco A; Forim, Moacir R; Silva, Luis F C; Coletta-Filho, Helvécio D; Machado, Marcos A; de Souza, Alessandra A

    2013-01-01

    Xylella fastidiosa is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it causes Citrus Variegated Chlorosis (CVC). The mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. In this study, we investigated the inhibitory effect of N-Acetylcysteine (NAC), a cysteine analogue used mainly to treat human diseases, on X. fastidiosa in different experimental conditions. Concentrations of NAC over 1 mg/mL reduced bacterial adhesion to glass surfaces, biofilm formation and the amount of exopolysaccharides (EPS). The minimal inhibitory concentration of NAC was 6 mg/mL. NAC was supplied to X. fastidiosa-infected plants in hydroponics, fertigation, and adsorbed to organic fertilizer (NAC-Fertilizer). HPLC analysis indicated that plants absorbed NAC at concentrations of 0.48 and 2.4 mg/mL but not at 6 mg/mL. Sweet orange plants with CVC symptoms treated with NAC (0.48 and 2.4 mg/mL) in hydroponics showed clear symptom remission and reduction in bacterial population, as analyzed by quantitative PCR and bacterial isolation. Experiments using fertigation and NAC-Fertilizer were done to simulate a condition closer to that normally is used in the field. For both, significant symptom remission and a reduced bacterial growth rate were observed. Using NAC-Fertilizer the lag for resurgence of symptoms on leaves after interruption of the treatment increased to around eight months. This is the first report of the anti-bacterial effect of NAC against a phytopathogenic bacterium. The results obtained in this work together with the characteristics of this molecule indicate that the use of NAC in agriculture might be a new and sustainable strategy for controlling plant pathogenic bacteria. PMID:24009716

  1. Benefits of supplementing an industrial waste anaerobic digester with energy crops for increased biogas production

    SciTech Connect

    Nges, Ivo Achu; Escobar, Federico; Fu Xinmei; Bjoernsson, Lovisa

    2012-01-15

    Highlights: Black-Right-Pointing-Pointer This study demonstrates the feasibility of co-digestion food industrial waste with energy crops. Black-Right-Pointing-Pointer Laboratory batch co-digestion led to improved methane yield and carbon to nitrogen ratio as compared to mono-digestion of industrial waste. Black-Right-Pointing-Pointer Co-digestion was also seen as a means of degrading energy crops with nutrients addition as crops are poor in nutrients. Black-Right-Pointing-Pointer Batch co-digestion methane yields were used to predict co-digestion methane yield in full scale operation. Black-Right-Pointing-Pointer It was concluded that co-digestion led an over all economically viable process and ensured a constant supply of feedstock. - Abstract: Currently, there is increasing competition for waste as feedstock for the growing number of biogas plants. This has led to fluctuation in feedstock supply and biogas plants being operated below maximum capacity. The feasibility of supplementing a protein/lipid-rich industrial waste (pig manure, slaughterhouse waste, food processing and poultry waste) mesophilic anaerobic digester with carbohydrate-rich energy crops (hemp, maize and triticale) was therefore studied in laboratory scale batch and continuous stirred tank reactors (CSTR) with a view to scale-up to a commercial biogas process. Co-digesting industrial waste and crops led to significant improvement in methane yield per ton of feedstock and carbon-to-nitrogen ratio as compared to digestion of the industrial waste alone. Biogas production from crops in combination with industrial waste also avoids the need for micronutrients normally required in crop digestion. The batch co-digestion methane yields were used to predict co-digestion methane yield in full scale operation. This was done based on the ratio of methane yields observed for laboratory batch and CSTR experiments compared to full scale CSTR digestion of industrial waste. The economy of crop-based biogas

  2. Exergy & economic analysis of biogas fueled solid oxide fuel cell systems

    NASA Astrophysics Data System (ADS)

    Siefert, Nicholas S.; Litster, Shawn

    2014-12-01

    We present an exergy and an economic analysis of a power plant that uses biogas produced from a thermophilic anaerobic digester (AD) to fuel a solid oxide fuel cell (SOFC). We performed a 4-variable parametric analysis of the AD-SOFC system in order to determine the optimal design operation conditions, depending on the objective function of interest. We present results on the exergy efficiency (%), power normalized capital cost ( kW-1), and the internal rate of return on investment, IRR, (% yr-1) as a function of the current density, the stack pressure, the fuel utilization, and the total air stoichiometric ratio. To the authors' knowledge, this is the first AD-SOFC paper to include the cost of the AD when conducting economic optimization of the AD-SOFC plant. Our calculations show that adding a new AD-SOFC system to an existing waste water treatment (WWT) plant could yield positives values of IRR at today's average electricity prices and could significantly out-compete other options for using biogas to generate electricity. AD-SOFC systems could likely convert WWT plants into net generators of electricity rather than net consumers of electricity while generating economically viable rates of return on investment if the costs of SOFC systems are within a factor of two of the DOE/SECA cost targets.

  3. Exergy & economic analysis of biogas fueled solid oxide fuel cell systems

    NASA Astrophysics Data System (ADS)

    Siefert, Nicholas S.; Litster, Shawn

    2014-12-01

    We present an exergy and an economic analysis of a power plant that uses biogas produced from a thermophilic anaerobic digester (AD) to fuel a solid oxide fuel cell (SOFC). We performed a 4-variable parametric analysis of the AD-SOFC system in order to determine the optimal design operation conditions, depending on the objective function of interest. We present results on the exergy efficiency (%), power normalized capital cost ($ kW-1), and the internal rate of return on investment, IRR, (% yr-1) as a function of the current density, the stack pressure, the fuel utilization, and the total air stoichiometric ratio. To the authors' knowledge, this is the first AD-SOFC paper to include the cost of the AD when conducting economic optimization of the AD-SOFC plant. Our calculations show that adding a new AD-SOFC system to an existing waste water treatment (WWT) plant could yield positives values of IRR at today's average electricity prices and could significantly out-compete other options for using biogas to generate electricity. AD-SOFC systems could likely convert WWT plants into net generators of electricity rather than net consumers of electricity while generating economically viable rates of return on investment if the costs of SOFC systems are within a factor of two of the DOE/SECA cost targets.

  4. A Critical Assessment of Microbiological Biogas to Biomethane Upgrading Systems.

    PubMed

    Rittmann, Simon K-M R

    2015-01-01

    Microbiological biogas upgrading could become a promising technology for production of methane (CH(4)). This is, storage of irregular generated electricity results in a need to store electricity generated at peak times for use at non-peak times, which could be achieved in an intermediate step by electrolysis of water to molecular hydrogen (H(2)). Microbiological biogas upgrading can be performed by contacting carbon dioxide (CO(2)), H(2) and hydrogenotrophic methanogenic Archaea either in situ in an anaerobic digester, or ex situ in a separate bioreactor. In situ microbiological biogas upgrading is indicated to require thorough bioprocess development, because only low volumetric CH(4) production rates and low CH(4) fermentation offgas content have been achieved. Higher volumetric production rates are shown for the ex situ microbiological biogas upgrading compared to in situ microbiological biogas upgrading. However, the ex situ microbiological biogas upgrading currently suffers from H(2) gas liquid mass transfer limitation, which results in low volumetric CH(4) productivity compared to pure H(2)/CO(2) conversion to CH(4). If waste gas utilization from biological and industrial sources can be shown without reduction in volumetric CH(4) productivity, as well as if the aim of a single stage conversion to a CH(4) fermentation offgas content exceeding 95 vol% can be demonstrated, ex situ microbiological biogas upgrading with pure or enrichment cultures of methanogens could become a promising future technology for almost CO(2)-neutral biomethane production. PMID:26337846

  5. In Situ 3D Segmentation of Individual Plant Leaves Using a RGB-D Camera for Agricultural Automation

    PubMed Central

    Xia, Chunlei; Wang, Longtan; Chung, Bu-Keun; Lee, Jang-Myung

    2015-01-01

    In this paper, we present a challenging task of 3D segmentation of individual plant leaves from occlusions in the complicated natural scene. Depth data of plant leaves is introduced to improve the robustness of plant leaf segmentation. The low cost RGB-D camera is utilized to capture depth and color image in fields. Mean shift clustering is applied to segment plant leaves in depth image. Plant leaves are extracted from the natural background by examining vegetation of the candidate segments produced by mean shift. Subsequently, individual leaves are segmented from occlusions by active contour models. Automatic initialization of the active contour models is implemented by calculating the center of divergence from the gradient vector field of depth image. The proposed segmentation scheme is tested through experiments under greenhouse conditions. The overall segmentation rate is 87.97% while segmentation rates for single and occluded leaves are 92.10% and 86.67%, respectively. Approximately half of the experimental results show segmentation rates of individual leaves higher than 90%. Nevertheless, the proposed method is able to segment individual leaves from heavy occlusions. PMID:26295395

  6. Effects of agricultural tillage and sediment accumulation on emergent plant communities in playa wetlands of the U.S. High Plains.

    PubMed

    O'Connell, Jessica L; Johnson, Lacrecia A; Daniel, Dale W; McMurry, Scott T; Smith, Loren M; Haukos, David A

    2013-05-15

    Identifying community assembly filters is a primary ecological aim. The High Plains, a 30 million ha short-grass eco-region, is intensely cultivated. Cultivation disturbance, including plowing and eroded soil deposition down-slope of plowing, influences plant composition in depressional wetlands, such as playas, within croplands. We evaluated influences of wetland cultivation and sediment deposition on plant composition in playas embedded within croplands (46 plowed and 32 unplowed) and native grasslands (79) across 6 High Plains' states. Sediment accumulation ranged from 7 to 78 cm in cropland and 1 to 35 cm in grassland playas. Deeper sediments and plowing each decreased wetland plant richness, 28% and 70% respectively in cropland wetlands. Sediment depth reduced richness 37% in small grasslands playas while it increased richness 22% in larger ones, suggesting moderate disturbance increased richness when there were nearby propagule sources. Sediment depth was unrelated to species richness in plowed wetlands, probably because plowing was a strong disturbance. Plowing removed perennial plants from vegetation communities. Sediment accumulation also influenced species composition in cropland playas, e.g., probability of Eleocharis atropurpurea increased with sediment depth, while probability of Panicum capillare decreased. In grassland playas, observed lighter sediment depths did not influence species composition after accounting for wetland area. Sediment accumulation and plowing shift wetland plant communities toward annual species and decrease habitat connectivity for wetland-dependent organisms in cropland playas over 39,000 and 23,400 ha respectively. Conservation practices lessening sediment accumulation include short-grass buffer strips surrounding wetlands. Further, wetland tillage, allowed under federal agricultural conservation programs, should be eliminated. PMID:23500104

  7. Impact of temperature on the dynamics of organic matter and on the soil-to-plant transfer of Cd, Zn and Pb in a contaminated agricultural soil.

    PubMed

    Cornu, Jean-Yves; Denaix, L; Lacoste, J; Sappin-Didier, V; Nguyen, C; Schneider, A

    2016-02-01

    Predicting the soil-to-plant transfer of metals in the context of global warming has become a major issue for food safety. It requires a better understanding of how the temperature alters the bioavailability of metals in cultivated soils. This study focuses on one agricultural soil contaminated by Cd, Zn and Pb. DGT measurements were performed at 10, 20 and 30 °C to assess how the bioavailability of metals was affected by a rise in soil temperature. A lettuce crop was cultivated in the same conditions to determine if the soil-to-plant transfer of metals increased with a rise in soil temperature. A gradual decline in Cd and Zn bioavailability was observed from 10 to 30 °C, which was attributed to more intense complexation of metals in the pore water at higher temperatures. Together with its aromaticity, the affinity of dissolved organic matter (DOM) for metals was indeed suspected to increase with soil temperature. One main output of the present work is a model which satisfactorily explains the thermal-induced changes in the characteristics of DOM reported in Cornu et al. (Geoderma 162:65-70, 2011) by assuming that the mineralization of initial aliphatic compounds followed a first-order reaction, increased with soil temperature according to the Arrhenius law, and due to a priming effect, led to the appearance of aromatic molecules. The soil-to-plant transfer of Cd and Zn was promoted at higher soil temperatures despite a parallel decrease in Cd and Zn bioavailability. This suggests that plant processes affect the soil-to-plant transfer of Cd and Zn the most when the soil temperature rises. PMID:26452657

  8. Relative contribution of oxygenated hydrocarbons to the total biogenic VOC emissions of selected mid-European agricultural and natural plant species

    NASA Astrophysics Data System (ADS)

    König, Georg; Brunda, Monika; Puxbaum, Hans; Hewitt, C. Nicholas; Duckham, S. Craig; Rudolph, Jochen

    Emission rates of more than 50 individual VOCs were determined for eight plant species and three different types of grass land typical for natural deciduous and agricultural vegetation in Austria. In addition to the emissions of isoprene and monoterpenes, 33 biogenic oxygenated volatile organic compounds (BOVOCs) were detected. Of these, 2-methyl-l-propanol, 1-butanal, 2-butanal, 1-pentanol, 3-pentanol, 1-hexanol, 6-methyl-5-hepten-2-one, butanal and ethylhexylacetate were observed for the first time as plant emissions. In terms of prevalence of one of the groups of emitted VOCs (isoprene, terpenes, BOVOCs) the grain plants wheat and rye, grape, oilseed rape and the decidous trees hombeam and birch could be classified as "BOVOC"-emitters. For the grass plots examined, BOVOCs and terpenes appear to be of equal importance. The emission rates of the total assigned organic plant emissions ranged from 0.01 μ g -1 h -1 for wheat to 0.8 μg g -1 h -1 for oak (based on dry leaf weight). Intercomparison with available data from other studies show that our emission rates are rather at the lower end of reported ranges. The influence of the stage of growth was examined for rye, rape (comparing emissions of blossoming and nonblossoming plants) and for grape (with and without fruit). Emission rate differences for different stages of growth varied from nondetectable for blossoming and nonblossoming rye to a factor of six for the grape with fruits vs grape without fruits (emission rate based on dry leaf weight). The major decidous tree in Austria (beech) is a terpene emitter, with the contribution of BOVOCs below 5% of the total assigned emissions of 0.2 μg g -1 h -1 for the investigations of 20°C.

  9. POTENTIAL ECOLOGICAL AND NONTARGET EFFECTS OF TRANSGENIC PLANT GENE PRODUCTS ON AGRICULTURE, SILVICULTURE, AND NATURAL ECOSYSTEMS: GENERAL INTRODUCTION

    EPA Science Inventory

    A symposium was held at the University of Maryland Nov. 30 - Dec. 2, 1992, to discuss transgenic plant risk assessment issues for measurement and identification of potential ecological and nontarget organism effects. he goal was to identify available scientific information and hi...

  10. Formulations of polymeric biodegradable low-cost foam by melt extrusion to deliver plant growth-promoting bacteria in agricultural systems.

    PubMed

    Marcelino, Paulo Ricardo Franco; Milani, Karina Maria Lima; Mali, Suzana; Santos, Odair José Andrade Pais Dos; de Oliveira, André Luiz Martinez

    2016-08-01

    The extrusion technology of blends formed by compounds with different physicochemical properties often results in new materials that present properties distinctive from its original individual constituents. Here, we report the use of melt extrusion of blends made from low-cost materials to produce a biodegradable foam suitable for use as an inoculant carrier of plant growth-promoting bacteria (PGPB). Six formulations were prepared with variable proportions of the raw materials; the resulting physicochemical and structural properties are described, as well as formulation performance in the maintenance of bacterial viability during 120 days of storage. Differences in blend composition influenced foam density, porosity, expansion index, and water absorption. Additionally, differences in the capability of sustaining bacterial viability for long periods of time were more related to the foam composition than to the resulting physicochemical characteristics. Microscopic analyses showed that the inoculant bacteria had firmly attached to the extruded material by forming biofilms. Inoculation assays using maize plants demonstrated that the bacteria attached to the extruded foams could survive in the soil for up to 10 days before maize sowing, without diminishing its ability to promote plant growth. The results presented demonstrate the viability of the new matrix as a biotechnological material for bacterial delivery not only in agriculture but also in other biotechnological applications, according to the selected bacterial strains. PMID:27147530

  11. Effectiveness of Rice Agricultural Waste, Microbes and Wetland Plants in the Removal of Reactive Black-5 Azo Dye in Microcosm Constructed Wetlands.

    PubMed

    Saba, Beenish; Jabeen, Madeeha; Khalid, Azeem; Aziz, Irfan; Christy, Ann D

    2015-01-01

    Azo dyes are commonly generated as effluent pollutants by dye using industries, causing contamination of surface and ground water. Various strategies are employed to treat such wastewater; however, a multi-faceted treatment strategy could be more effective for complete removal of azo dyes from industrial effluent than any single treatment. In the present study, rice husk material was used as a substratum in two constructed wetlands (CWs) and augmented with microorganisms in the presence of wetland plants to effectively treat dye-polluted water. To evaluate the efficiency of each process the study was divided into three levels, i.e., adsorption of dye onto the substratum, phytoremediation within the CW and then bioremediation along with the previous two processes in the augmented CW. The adsorption process was helpful in removing 50% dye in presence of rice husk while 80% in presence of rice husk biocahr. Augmentation of microorganisms in CW systems has improved dye removal efficiency to 90%. Similarly presence of microorganisms enhanced removal of total nitrogen (68% 0 and Total phosphorus (75%). A significant improvement in plant growth was also observed by measuring plant height, number of leaves and leave area. These findings suggest the use of agricultural waste as part of a CW substratum can provide enhanced removal of textile dyes. PMID:25849115

  12. The effect of storage conditions on microbial community composition and biomethane potential in a biogas starter culture.

    PubMed

    Hagen, Live Heldal; Vivekanand, Vivekanand; Pope, Phillip B; Eijsink, Vincent G H; Horn, Svein J

    2015-07-01

    A new biogas process is initiated by adding a microbial community, typically in the form of a sample collected from a functional biogas plant. This inoculum has considerable impact on the initial performance of a biogas reactor, affecting parameters such as stability, biogas production yields and the overall efficiency of the anaerobic digestion process. In this study, we have analyzed changes in the microbial composition and performance of an inoculum during storage using barcoded pyrosequencing of bacterial and archaeal 16S ribosomal RNA (rRNA) genes, and determination of the biomethane potential, respectively. The inoculum was stored at room temperature, 4 and -20 °C for up to 11 months and cellulose was used as a standard substrate to test the biomethane potential. Storage up to 1 month resulted in similar final methane yields, but the rate of methane production was reduced by storage at -20 °C. Longer storage times resulted in reduced methane yields and slower production kinetics for all storage conditions, with room temperature and frozen samples consistently giving the best and worst performance, respectively. Both storage time and temperature affected the microbial community composition and methanogenic activity. In particular, fluctuations in the relative abundance of Bacteroidetes were observed. Interestingly, a shift from hydrogenotrophic methanogens to methanogens with the capacity to perform acetoclastic methanogensis was observed upon prolonged storage. In conclusion, this study suggests that biogas inocula may be stored up to 1 month with low loss of methanogenic activity, and identifies bacterial and archaeal species that are affected by the storage. PMID:25947246

  13. Oxidation of siloxanes during biogas combustion and nanotoxicity of Si-based particles released to the atmosphere.

    PubMed

    Tansel, Berrin; Surita, Sharon C

    2014-01-01

    Siloxanes have been detected in the biogas produced at municipal solid waste landfills and wastewater treatment plants. When oxidized, siloxanes are converted to silicon oxides. The objectives of this study were to evaluate the transformation of siloxanes and potential nanotoxicity of Si-based particles released to the atmosphere from the gas engines which utilize biogas. Data available from nanotoxicity studies were used to assess the potential health risks associated with the inhalation exposure to Si-based nanoparticles. Silicon dioxide formed from siloxanes can range from 5 nm to about 100 nm in diameter depending on the combustion temperature and particle clustering characteristics. In general, silicon dioxide particles formed during from combustion process are typically 40-70 nm in diameter and can be described as fibrous dusts and as carcinogenic, mutagenic, astmagenic or reproductive toxic (CMAR) nanoparticles. Nanoparticles deposit in the upper respiratory system, conducting airways, and the alveoli. Size ranges between 5 and 50 nm show effective deposition in the alveoli where toxic effects are higher. In this study the quantities for the SiO₂ formed and release during combustion of biogas were estimated based on biogas utilization characteristics (gas compositions, temperature). The exposure to Si-based particles and potential effects in humans were analyzed in relation to their particle size, release rates and availability in the atmosphere. The analyses showed that about 54.5 and 73 kg/yr of SiO₂ can be released during combustion of biogas containing D4 and D5 at 14.1 mg/m(3) (1 ppm) and 15.1 mg/m(3) (1ppm), respectively, per MW energy yield. PMID:24355797

  14. Estimating biogas production of biologically treated municipal solid waste.

    PubMed

    Scaglia, Barbara; Confalonieri, Roberto; D'Imporzano, Giuliana; Adani, Fabrizio

    2010-02-01

    In this work, a respirometric approach, i.e., Dynamic Respiration Index (DRI), was used to predict the anaerobic biogas potential (ABP), studying 46 waste samples coming directly from MBT full-scale plants. A significant linear regression model was obtained by a jackknife approach: ABP=(34.4+/-2.5)+(0.109+/-0.003).DRI. The comparison of the model of this work with those of the previous works using a different respirometric approach (Sapromat-AT(4)), allowed obtaining similar results and carrying out direct comparison of different limits to accept treated waste in landfill, proposed in the literature. The results indicated that on an average, MBT treatment allowed 56% of ABP reduction after 4weeks of treatment, and 79% reduction after 12weeks of treatment. The obtainment of another regression model allowed transforming Sapromat-AT(4) limit in DRI units, and achieving a description of the kinetics of DRI and the corresponding ABP reductions vs. MBT treatment-time. PMID:19783431

  15. Effects of climate change on agricultural-plant pests. Volume II, Part 10 of environmental and societal consequences of a possible CO/sub 2/-induced climate change

    SciTech Connect

    Haynes, D.L.

    1982-10-01

    Plant pests and their community of biotic cohorts respond to climatic changes, whether temporal aberrations or long term shifts. How they respond depends on the magnitude of the change and the ability of the species to tolerate or adapt to the new environment. Scientists see several climatological scenarios concerning the increase of atmospheric CO/sub 2/ and ambient temperature. Those who foresee a slow incremental raising of temperatures base their predictions mainly on the available empirical evidence and the notion that long term weather is basically a cyclical phenomena that continually adjusts and readjusts through time. The other scenario interprets the available empirical data as a gradual buildup that pushes the climatic picture towards a threshold or a trigger point that, once arrived at, is irreversible and dramatic. This paper explores the possible climatic scenarios as they relate to the ecological principles that affect pest abundance and the distribution and impact on domestic and international agriculture.

  16. Anaerobic co-digestion of sewage sludge and primary clarifier skimmings for increased biogas production.

    PubMed

    Alanya, S; Yilmazel, Y D; Park, C; Willis, J L; Keaney, J; Kohl, P M; Hunt, J A; Duran, M

    2013-01-01

    The objective of the study was to identify the impact of co-digesting clarifier skimmings on the overall methane generation from the treatment plant and additional energy value of the increased methane production. Biogas production from co-digesting clarifier skimmings and sewage sludge in pilot-scale fed-batch mesophilic anaerobic digesters has been evaluated. The digester was fed with increasing quantities of clarifier skimmings loads: 1.5, 2.6, 3.5 and 7.0 g COD equivalent/(L·d) (COD: chemical oxygen demand). Average volatile solids reduction of 65% was achieved in the scum-fed digester, compared with 51% in the control digester. Average 69% COD removal was achieved at highest scum loading (7 g COD eq/(L·d)) with approximate methane yield of 250 L CH(4)/kg COD fed (4 ft(3)/lb COD fed). The results show that scum as co-substrate in anaerobic digestion systems improves biogas yields while a 29% increase in specific CH(4) yield could be achieved when scum load is 7 g COD eq/(L·d). Based on the pilot-scale study results and full-scale data from South East Water Pollution Control Plant and Northeast Water Pollution Control Plant the expected annual energy recovery would be approximately 1.7 billion BTUs or nearly 0.5 million kWh. PMID:23128636

  17. Piper nigrum Leaf and Stem Assisted Green Synthesis of Silver Nanoparticles and Evaluation of Its Antibacterial Activity Against Agricultural Plant Pathogens

    PubMed Central

    Paulkumar, Kanniah; Gnanajobitha, Gnanadhas; Vanaja, Mahendran; Rajeshkumar, Shanmugam; Malarkodi, Chelladurai; Pandian, Kannaiyan; Annadurai, Gurusamy

    2014-01-01

    Utilization of biological materials in synthesis of nanoparticles is one of the hottest topics in modern nanoscience and nanotechnology. In the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract of Piper nigrum. The synthesized nanoparticle was characterized by UV-vis spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), energy dispersive X-ray analysis (EDAX), and Fourier Transform Infrared Spectroscopy (FTIR). The observation of the peak at 460 nm in the UV-vis spectra for leaf- and stem-synthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. Further, XRD analysis has been carried out to confirm the crystalline nature of the synthesized silver nanoparticles. The TEM images show that the leaf- and stem-synthesized silver nanoparticles were within the size of about 7–50 nm and 9–30 nm, respectively. The FTIR analysis was performed to identify the possible functional groups involved in the synthesis of silver nanoparticles. Further, the antibacterial activity of the green-synthesized silver nanoparticles was examined against agricultural plant pathogens. The antibacterial property of silver nanoparticles is a beneficial application in the field of agricultural nanotechnology. PMID:24558336

  18. [Influence of bioleaching on dewaterability of cattle biogas slurry].

    PubMed

    Zhou, Jun; Zhou, Li-Xiang; Liu, Fen-Wu; Ren, Yun; Wang, Dian-Zhan

    2011-11-01

    The dewaterability of cattle biogas slurry facilitated by bioleaching was investigated through batch experiments with co-inoculation of different Acidophilic thiobacilli (Acidithiobacillus thiooxidans TS6 or Acidithiobacillus ferrooxidans LX5). The experiment was set the following 5 treatments: (1) original biogas slurry (CK), (2) 4 g x L(-1) Fe(2+) (uninoculation), (3)2 g x L(-1) S(0) + 25 mL A. t, (4) 4 g x L(-1) Fe(2+) + 25 mL A. f and (5) 2 g x L(-1) S(0) + 4 g x L(-1) Fe(2+) + 12.5 mL A. t + 12.5 mL A. f. During bioleaching, dynamic changes of pH, ORP, Fe(2+), F(3+), total Fe, the settleability, the turbidity of the supernatant after settling for 12 h, and the dewaterability (expressed as specific resistance to filtration gamma or capillary suction time, CST) of biogas slurry were monitored. Results show that specific resistance gamma and CST of bioleached biogas slurry are reduced drastically for the treatments of original biogas slurry spiked with only Fe(2+), the treatment of original biogas slurry co-spiked with Fe(2+) and Acidithiobacillus ferrooxidans LX5, and the treatment of original biogas slurry co-spiked with Fe(2+), S(0) and two Acidophilic thiobacilli. Taking the dewaterability, settleability, the turbidity of the supernatant fluid after settle 12 h and economical cost into account, the treatment of original biogas slurry co-spiked with Fe(2+) and Acidithiobacillus ferrooxidans LX5 is the most suitable pattern for cattle biogas slurry bioleaching. After bioleaching, 1.14% of organic matter, 0.09% of N, 0.05% of P, and 0.1% of K are lost in the bioleaching process, but it don't affect its fertilizer efficiency. Meanwhile, the 63.2% of Cu and 91.3% of Zn are removed from the biogas slurry, and elimination efficiencies of total coliforms in bioleached slurry exceed 99%. This study might provide a new approach for treatment and disposal of biogas slurry. PMID:22295641

  19. Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation

    PubMed Central

    Usack, Joseph G.; Spirito, Catherine M.; Angenent, Largus T.

    2012-01-01

    Anaerobic digestion (AD) is a bioprocess that is commonly used to convert complex organic wastes into a useful biogas with methane as the energy carrier 1-3. Increasingly, AD is being used in industrial, agricultural, and municipal waste(water) treatment applications 4,5. The use of AD technology allows plant operators to reduce waste disposal costs and offset energy utility expenses. In addition to treating organic wastes, energy crops are being converted into the energy carrier methane 6,7. As the application of AD technology broadens for the treatment of new substrates and co-substrate mixtures 8, so does the demand for a reliable testing methodology at the pilot- and laboratory-scale. Anaerobic digestion systems have a variety of configurations, including the continuously stirred tank reactor (CSTR), plug flow (PF), and anaerobic sequencing batch reactor (ASBR) configurations 9. The CSTR is frequently used in research due to its simplicity in design and operation, but also for its advantages in experimentation. Compared to other configurations, the CSTR provides greater uniformity of system parameters, such as temperature, mixing, chemical concentration, and substrate concentration. Ultimately, when designing a full-scale reactor, the optimum reactor configuration will depend on the character of a given substrate among many other nontechnical considerations. However, all configurations share fundamental design features and operating parameters that render the CSTR appropriate for most preliminary assessments. If researchers and engineers use an influent stream with relatively high concentrations of solids, then lab-scale bioreactor configurations cannot be fed continuously due to plugging problems of lab-scale pumps with solids or settling of solids in tubing. For that scenario with continuous mixing requirements, lab-scale bioreactors are fed periodically and we refer to such configurations as continuously stirred anaerobic digesters (CSADs). This article

  20. The PC/Ecuador biogas program: considerations for future development

    SciTech Connect

    Warpeha, P.R.

    1980-06-28

    Biogas, the production of methane fuel and fertilizer through the process of controlled anaerobic decomposition, has been one of the most controversial of the new renewable energy technologies. The integrated approach to fuel and fertilizer production, conservation and sanitation on a decentralized community level potentially holds great promise as a truly appropriate technology for rural development. The report presents the research, development, and demonstration of biogas technology conducted by the Peace Corps in Ecuador for the past six years.

  1. Kinetics of biogas production in Anaerobic Filters.

    PubMed

    Krümpel, Johannes; Schäufele, Friedrich; Schneider, Johannes; Jungbluth, Thomas; Zielonka, Simon; Lemmer, Andreas

    2016-01-01

    This study investigates methane production kinetics from individual volatile fatty acids (VFA) in an Upflow Anaerobic Filter (AF). 1gCOD in the form of acetic (HAc), propionic (HPr) or butyric acid (HBu) was injected into the AF while operating at an organic loading rate (OLRCOD) of 3.5gL(-1)d(-1). A new method is introduced to separate gas production of the baseload from the product formation of VFA degradation after the injection. The lag phase, fractional rate of gas production and half-life has been determined for the methane production of the three VFAs. The half-lives were in the order HAcbiogas production, a vital approach that provides the transforming energy market with balancing power. PMID:26492176

  2. Life cycle assessment of biogas upgrading technologies.

    PubMed

    Starr, Katherine; Gabarrell, Xavier; Villalba, Gara; Talens, Laura; Lombardi, Lidia

    2012-05-01

    This article evaluates the life cycle assessment (LCA) of three biogas upgrading technologies. An in-depth study and evaluation was conducted on high pressure water scrubbing (HPWS), as well as alkaline with regeneration (AwR) and bottom ash upgrading (BABIU), which additionally offer carbon storage. AwR and BABIU are two novel technologies that utilize waste from municipal solid waste incinerators - namely bottom ash (BA) and air pollution control residues (APC) - and are able to store CO(2) from biogas through accelerated carbonation processes. These are compared to high pressure water scrubbing (HPWS) which is a widely used technology in Europe. The AwR uses an alkaline solution to remove the CO(2) and then the solution - rich in carbonate and bicarbonate ions - is regenerated through carbonation of APC. The BABIU process directly exposes the gas to the BA to remove and immediately store the CO(2), again by carbonation. It was determined that the AwR process had an 84% higher impact in all LCA categories largely due to the energy intensive production of the alkaline reactants. The BABIU process had the lowest impact in most categories even when compared to five other CO(2) capture technologies on the market. AwR and BABIU have a particularly low impact in the global warming potential category as a result of the immediate storage of the CO(2). For AwR, it was determined that using NaOH instead of KOH improves its environmental performance by 34%. For the BABIU process the use of renewable energies would improve its impact since accounts for 55% of the impact. PMID:22230660

  3. Flame Stabilization on Microscopic Scale of Wet Biogas with Microflame

    NASA Astrophysics Data System (ADS)

    Ida, Tamio; Fuchihata, Manabu; Mizuno, Satoru

    Harvesting, transportation, energy conversion and the high-efficient utilization, cascade method and market formation besides become with the indispensable element in order to utilize the biomass resource. There are two type biogases; it is gasified gas from dried biomass by partially combustion and wet biogas from wet biomass by methane fermentation, especially from the livestock excrement resources. This paper discusses an experimental study for flame stabilization on microscopic scale with wet biogas (mainly 0.6CH4+0.4CO2). In this study, the microflame with the wet biogas fuels are formed by the diffusion flame on the coppered straight pipes of inner diameter 0.02mm ˜ 1.5mm. This study is obtained stability mapping on microscopic scale of formed microflame by wet biogas fuels. The flame stability limit conditions on microscopic scale of wet biogas is drawn with blow off and extinction flame double limit lines. It is suggested that minimum mixing spatial scale change by the each mixing ratio of the wet biogas.

  4. Large-scale sequestration of atmospheric carbon via plant roots in natural and agricultural ecosystems: why and how

    PubMed Central

    Kell, Douglas B.

    2012-01-01

    The soil holds twice as much carbon as does the atmosphere, and most soil carbon is derived from recent photosynthesis that takes carbon into root structures and further into below-ground storage via exudates therefrom. Nonetheless, many natural and most agricultural crops have roots that extend only to about 1 m below ground. What determines the lifetime of below-ground C in various forms is not well understood, and understanding these processes is therefore key to optimising them for enhanced C sequestration. Most soils (and especially subsoils) are very far from being saturated with organic carbon, and calculations show that the amounts of C that might further be sequestered (http://dbkgroup.org/carbonsequestration/rootsystem.html) are actually very great. Breeding crops with desirable below-ground C sequestration traits, and exploiting attendant agronomic practices optimised for individual species in their relevant environments, are therefore important goals. These bring additional benefits related to improvements in soil structure and in the usage of other nutrients and water. PMID:22527402

  5. N2O and CH4-emissions from energy crops - Can the use of organic fertilizers in form of biogas digestate be considered as a real alternative? Results from a three and a half year multi-site field study of energy crops fertilized with biogas digestate in so

    NASA Astrophysics Data System (ADS)

    Heintze, Gawan

    2016-04-01

    Gawan Heintze1,2, Matthias Drösler1, Ulrike Hagemann3and Jürgen Augustin3 1University of Applied Sciences Weihenstephan-Triesdorf, Chair of Vegetation Ecology, Weihenstephaner Berg 4, 85354 Freising, Germany 2Technische Universität München, Chair of Plant Nutrition, Emil-Ramann-Str. 2, 85354 Freising, Germany 3Leibniz Centre for Agricultural Landscape Research (ZALF), Eberswalder Straße 84, 15374 Müncheberg, Germany Together with industrial process-related emissions (8.1%) the actual GHG emissions from agriculture (7.5% - 70 million tones (Mt) of carbon dioxide (CO2)-equivalents) representing after energy-related emissions from combustion processes of fossil fuels (83.7%) the second largest budget of the Germany-wide total emissions per year. To reduce the EU's CO2 emissions by 20% by 2020 the cultivation of energy crops for biogas production, ideally coupled to a subsequent return of the resulting residues in form of biogas digestate is intended as one key element in the pathway of renewable energy production. Despite an increasing cultivation of energy crops for the production of biogas aiming to reduce the overall climate impact of the agricultural sector, it is still largely unknown how the application of ammonia-rich organic digestate effects field N2O emissions. Therefore, the collaborative research project "potential for reducing the release of climate-relevant trace gases in the cultivation of energy crops for the production of biogas" was launched. The main objective of the study was to determine an improved process understanding and to quantify the influence of mineral nitrogen fertilization, biogas digestate application, crop type and crop rotation, to gain precise and generalizable statements on the exchange of trace gases like nitrous oxide (N2O) and methane (CH4) on the resulting climate impact. Gas fluxes of N2O and CH4 were measured for three and a half years on two differently managed sites in maize monoculture with different applied organic

  6. Screening of plant growth-promoting traits in arsenic-resistant bacteria isolated from agricultural soil and their potential implication for arsenic bioremediation.

    PubMed

    Das, Suvendu; Jean, Jiin-Shuh; Kar, Sandeep; Chou, Mon-Lin; Chen, Chien-Yen

    2014-05-15

    Twelve arsenic (As)-resistant bacteria (minimum inhibitory concentration ranging from 10 to 30mM and 150 to 320mM for As(III) and As(V), respectively) were isolated from the agricultural soil of the Chianan Plain in southwestern Taiwan using enrichment techniques. Eight isolates capable of oxidizing As(III) (rate of oxidation from 0.029 to 0.059μMh(-1) 10(-9) cell) and exhibiting As(III)-oxidase enzyme activity belong to Pseudomonas, Acinetobacter, Klebsiella and Comamonas genera, whereas four isolates that did not show As(III)-oxidizing activity belong to Geobacillus, Bacillus, Paenibacillus, and Enterobacter genera. Assessment of the parameters of plant growth promotion revealed that Pseudomonas sp. ASR1, ASR2 and ASR3, Geobacillus sp. ASR4, Bacillus sp. ASR5, Paenibacillus sp. ASR6, Enterobacter sp. ASR10 and Comamonas sp. ASR11, and ASR12 possessed some or all of the studied plant growth-promoting traits, including phosphate-solubilization, siderophore, IAA-like molecules and ACC deaminase production. In addition, the ability of As-resistant isolates to grow over wide ranges of pH and temperatures signify their potential application for sustainable bioremediation of As in the environment. PMID:24685527

  7. Recent range expansion and agricultural landscape heterogeneity have only minimal effect on the spatial genetic structure of the plant pathogenic fungus Mycosphaerella fijiensis.

    PubMed

    Rieux, A; De Lapeyre De Bellaire, L; Zapater, M-F; Ravigne, V; Carlier, J

    2013-01-01

    Understanding how geographical and environmental features affect genetic variation at both the population and individual levels is crucial in biology, especially in the case of pathogens. However, distinguishing between these factors and the effects of historical range expansion on spatial genetic structure remains challenging. In the present study, we investigated the case of Mycosphaerella fijiensis-a plant pathogenic fungus that has recently colonized an agricultural landscape characterized by the presence of potential barriers to gene flow, including several commercial plantations in which disease control practises such as the use of fungicides are applied frequently, and low host density areas. We first genotyped 300 isolates sampled at a global scale on untreated plants in two dimensions over a 50 × 80-km area. Using two different clustering algorithms, no genetic structure was detected in the studied area, suggesting expansion of large populations and/or no influence of potential barriers. Second, we investigated the potential effect of disease control practises on M. fijiensis diversity by comparing populations sampled in commercial vs food-crop plantations. At this local scale, we detected significantly higher allelic richness inside commercial plantations compared with the surrounding food-crop plantation populations. Analysis of molecular variance indicated that 99% of the total genetic variance occurred within populations. We discuss the suggestion that high population size and/or high migration rate between populations might be responsible for the absence of any effect of disease control practises on genetic diversity and differentiation. PMID:22990310

  8. Photochemistry in Power Plant and Urban Plumes over Forested and Agricultural Regions during SOS (1990s) and SENEX (2013) field intensives (Invited)

    NASA Astrophysics Data System (ADS)

    Trainer, M.; Frost, G. J.; Kim, S.; Ryerson, T. B.; Pollack, I. B.; Roberts, J. M.; Veres, P. R.; Flocke, F. M.; Neuman, J. A.; Nowak, J. B.; Nenes, A.; Warneke, C.; Graus, M.; Gilman, J.; Lerner, B. M.; Kuster, W.; Atlas, E. L.; Hanisco, T. F.; Wolfe, G. M.; Keutsch, F. N.; Kaiser, J.; Lee, Y.; Brock, C. A.; Middlebrook, A. M.; Liao, J.; Welti, A.; Parrish, D. D.; Fehsenfeld, F. C.; De Gouw, J. A.

    2013-12-01

    Extensive forested regions of the southeastern United States show high emissions of biogenic reactive hydrocarbons such as isoprene, while emissions of these compounds are typically much lower from agricultural areas. The Southern Oxidant Study (SOS) field intensives during the 1990s contributed to an improved understanding of ozone (O3) formation resulting from nitrogen oxides (NOx) emitted from urban areas and power plants in the presence and absence of the biogenic hydrocarbons. Decreases in NOx emissions from power plants and urban areas have contributed to the widespread reduction of ambient O3 over the southeastern US during the past two decades. Measurements of Volatile Organic Compounds (VOCs), NOx, and their reaction products made at successive distances downwind of emission sources during the SOS (1999) and the Southeast Nexus (SENEX, 2013) campaigns reflect the modulation of the photochemical processing of biogenic VOCs by ambient NOx concentrations. The results constrain the ambient levels of HOx radicals as a function of NOx, and they reflect the mechanisms of the coupling between anthropogenic and biogenic emissions that form species such as ozone, formaldehyde, PeroxyAcetic Nitric anhydride (PAN), nitric acid, as well as, inorganic and organic aerosols.

  9. Lunar agriculture in Mesoamerica

    NASA Astrophysics Data System (ADS)

    Iwaniszewski, S.

    Through the moon' s role in choosing the proper time for planting, harvesting and woodcutting is widely attested in ethnographic reports, the cultural logic and structure of actions by which this celestial body is perceived and used has not been satisfactorily explained. The aim of this paper is to offer such an explanatory framework within which the role of the moon in the agricultural cycle may be explained. My examples of the beliefs about lunar agriculture derive from the Mesoamerican cultural tradition.

  10. A comparison of land-sharing and land-sparing strategies for plant richness conservation in agricultural landscapes.

    PubMed

    Egan, J Franklin; Mortensen, David A

    2012-03-01

    Strategies for conserving plant diversity in agroecosystems generally focus on either expanding land area in non-crop habitat or enhancing diversity within crop fields through changes in within-field management practices. In this study, we compare effects on landscape-scale species richness from such land-sharing or land-sparing strategies. We collected data in arable field, grassland, pasture, and forest habitat types (1.6 ha sampled per habitat type) across a 100-km2 region of farmland in Lancaster County, Pennsylvania, USA. We fitted species-area relationships (SARs) for each habitat type and then combined extrapolations from the curves with estimates of community overlap to estimate richness in a 314.5-ha landscape. We then modified these baseline estimates by adjusting parameters in the SAR models to compare potential effects of land-sharing and land-sparing conservation practices on landscape richness. We found that species richness of the habitat types showed a strong inverse relationship to the relative land area of each type in the region, with 89 species in arable fields (66.5% of total land area), 153 in pastures (6.7%), 196 in forests (5.2%), and 213 in grasslands (2.9%). Relative to the baseline scenario, major changes in the richness of arable fields produced gains in landscape-scale richness comparable to a conversion of 3.1% of arable field area into grassland habitat. Sensitivity analysis of our model indicated that relative gains from land sparing would be greatest in landscapes with a low amount of non-crop habitat in the baseline scenario, but that in more complex landscapes land sharing would provide greater gains. These results indicate that the majority of plant species in agroecosystems are found in small fragments of non-crop habitat and suggest that, especially in landscapes with little non-crop habitat, richness can be more readily conserved through land-sparing approaches. PMID:22611847

  11. Biosurfactants in agriculture.

    PubMed

    Sachdev, Dhara P; Cameotra, Swaranjit S

    2013-02-01

    Agricultural productivity to meet growing demands of human population is a matter of great concern for all countries. Use of green compounds to achieve the sustainable agriculture is the present necessity. This review highlights the enormous use of harsh surfactants in agricultural soil and agrochemical industries. Biosurfactants which are reported to be produced by bacteria, yeasts, and fungi can serve as green surfactants. Biosurfactants are considered to be less toxic and eco-friendly and thus several types of biosurfactants have the potential to be commercially produced for extensive applications in pharmaceutical, cosmetics, and food industries. The biosurfactants synthesized by environmental isolates also has promising role in the agricultural industry. Many rhizosphere and plant associated microbes produce biosurfactant; these biomolecules play vital role in motility, signaling, and biofilm formation, indicating that biosurfactant governs plant-microbe interaction. In agriculture, biosurfactants can be used for plant pathogen elimination and for increasing the bioavailability of nutrient for beneficial plant associated microbes. Biosurfactants can widely be applied for improving the agricultural soil quality by soil remediation. These biomolecules can replace the harsh surfactant presently being used in million dollar pesticide industries. Thus, exploring biosurfactants from environmental isolates for investigating their potential role in plant growth promotion and other related agricultural applications warrants details research. Conventional methods are followed for screening the microbial population for production of biosurfactant. However, molecular methods are fewer in reaching biosurfactants from diverse microbial population and there is need to explore novel biosurfactant from uncultured microbes in soil biosphere by using advanced methodologies like functional metagenomics. PMID:23280539

  12. Thermal efficiency and environmental performances of a biogas-diesel stationary engine.

    PubMed

    Bilcan, A; Le Corre, O; Delebarre, A

    2003-09-01

    Municipal and agricultural waste, and sludge from wastewater treatment represent a large source of pollution. Gaseous fuels can be produced from waste decomposition and then used to run internal combustion engines for power and heat generation. The present paper focuses on thermal efficiency and environmental performances of dual-fuel engines fuelled with biogas. Experiments have been carried out on a Lister-Petter single cylinder diesel engine, modified for dual-fuel operation. Natural gas was first used as the primary fuel. An empirical correlation was determined to predict the engine load for a given mass flow rate for the pilot fuel (diesel) and for the primary fuel (natural gas). That correlation has then been tested for three synthesized biogas compositions. Computations were performed and the error was estimated to be less than 10%. Additionally, NOx and CO2 contents were measured from exhaust gases. Based on exhausts gas temperature, the activation energy and the pre-exponential factor of an Arrhenius law were then proposed, resulting in a simpler mean to predict NOx. PMID:14599150

  13. Grassland agriculture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Agriculture in grassland environments is facing multiple stresses from: shifting demographics, declining and fragmented agricultural landscapes, declining environmental quality, variable and changing climate, volatile and increasing energy costs, marginal economic returns, and globalization. Degrad...

  14. Agricultural Production.

    ERIC Educational Resources Information Center

    Lehigh County Area Vocational-Technical School, Schnecksville, PA.

    This brochure describes the philosophy and scope of a secondary-level course in agricultural production. Addressed in the individual units of the course are the following topics: careers in agriculture and agribusiness, animal science and livestock production, agronomy, agricultural mechanics, supervised occupational experience programs, and the…

  15. Dry fermentation of agricultural residues

    NASA Astrophysics Data System (ADS)

    Jewell, W. J.; Chandler, J. A.; Dellorto, S.; Fanfoni, K. J.; Fast, S.; Jackson, D.; Kabrick, R. M.

    1981-09-01

    A dry fermentation process is discussed which converts agricultural residues to methane, using the residues in their as produced state. The process appears to simplify and enhance the possibilities for using crop residues as an energy source. The major process variables investigated include temperature, the amount and type of inoculum, buffer requirements, compaction, and pretreatment to control the initial available organic components that create pH problems. A pilot-scale reactor operation on corn stover at a temperature of 550 C, with 25 percent initial total solids, a seed-to-feed ratio of 2.5 percent, and a buffer-to-feed ratio of 8 percent achieved 33 percent total volatile solids destruction in 60 days. Volumetric biogas yields from this unit were greater than 1 vol/vol day for 12 days, and greater than 0.5 vol/vol day for 32 days, at a substrate density of 169 kg/m (3).

  16. Chitin amendment increases soil suppressiveness toward plant pathogens and modulates the actinobacterial and oxalobacteraceal communities in an experimental agricultural field.

    PubMed

    Cretoiu, Mariana Silvia; Korthals, Gerard W; Visser, Johnny H M; van Elsas, Jan Dirk

    2013-09-01

    A long-term experiment on the effect of chitin addition to soil on the suppression of soilborne pathogens was set up and monitored for 8 years in an experimental field, Vredepeel, The Netherlands. Chitinous matter obtained from shrimps was added to soil top layers on two different occasions, and the suppressiveness of soil toward Verticillium dahliae, as well as plant-pathogenic nematodes, was assessed, in addition to analyses of the abundances and community structures of members of the soil microbiota. The data revealed that chitin amendment had raised the suppressiveness of soil, in particular toward Verticillium dahliae, 9 months after the (second) treatment, extending to 2 years following treatment. Moreover, major effects of the added chitin on the soil microbial communities were detected. First, shifts in both the abundances and structures of the chitin-treated soil microbial communities, both of total soil bacteria and fungi, were found. In addition, the abundances and structures of soil actinobacteria and the Oxalobacteraceae were affected by chitin. At the functional gene level, the abundance of specific (family-18 glycoside hydrolase) chitinase genes carried by the soil bacteria also revealed upshifts as a result of the added chitin. The effects of chitin noted for the Oxalobacteraceae were specifically related to significant upshifts in the abundances of the species Duganella violaceinigra and Massilia plicata. These effects of chitin persisted over the time of the experiment. PMID:23811512

  17. Preliminary energy sector assessments of Jamacica. Volume III: renewable energy. Part III: biogas applications

    SciTech Connect

    Not Available

    1980-01-01

    The study considers the feasibility of biogas generation and applications. Biogas is a high grade fuel produced by anaerobic digestion of organic matter such as farm crops or animal manure and is useful for cooking and other purposes.

  18. Sensor needs for agricultural and carbon management

    Technology Transfer Automated Retrieval System (TEKTRAN)

    There is a wide variety of sensors and platforms available for agricultural and carbon management. Two areas of concern are monitoring plant nutrients and crop residue over agricultural watersheds. Excess plant nutrients and agricultural chemicals may runoff into the water supply, degrading water ...

  19. Agriculture Cluster Brief. Vocational Education in Oregon.

    ERIC Educational Resources Information Center

    Galbraith, Gordon

    This guide sets forth minimum approval criteria for vocational agriculture cluster programs in Oregon. The agriculture cluster program includes instruction in six areas: animal science, soil science, plant science, agricultural economics, agriculture mechanics, and leadership development. The information in the guide is intended for use by…

  20. Integrated biomass energy system for Illinois agriculture. Final report

    SciTech Connect

    Day, D.L.; Steinberg, M.P.; Rodda, E.D.; Hunt, D.R.

    1988-05-01

    There is considerable interest in the corn belt region of the USA for producing grain alcohol on farms as liquid fuel for tractors, combines, and trucks used in farming operations. Opportunities appear to exist for fuel-alcohol production on a small scale by individual farmers and local groups. Two major energy consumers in beverage-type alcohol plants are the drying of stillage residues and the fueling the distillation plant. These large energy costs have roused interest in product conservation and fuel-saving methods by using spent stillage as livestock feed and using manure-produced biogas as the process fuel for the alcohol plant. Sludge from the biogas plant also can be distributed onto the cropland to furnish some of the needed crop nutrients.

  1. Agricultural Waste.

    PubMed

    Xue, Ling; Zhang, Panpan; Shu, Huajie; Chang, Chein-Chi; Wang, Renqing; Zhang, Shuping

    2016-10-01

    In recent years, the quantity of agricultural waste has been rising rapidly all over the world. As a result, the environmental problems and negative impacts of agricultural waste are drawn more and more attention. Therefore, there is a need to adopt proper approaches to reduce and reuse agricultural waste. This review presented about 200 literatures published in 2015 relating to the topic of agricultural waste. The review examined research on agricultural waste in 2015 from the following four aspects: the characterization, reuse, treatment, and management. Researchers highlighted the importance to reuse agricultural waste and investigated the potential to utilize it as biofertilizers, cultivation material, soil amendments, adsorbent, material, energy recycling, enzyme and catalyst etc. The treatment of agricultural waste included carbonization, biodegradation, composting hydrolysis and pyrolysis. Moreover, this review analyzed the differences of the research progress in 2015 from 2014. It may help to reveal the new findings and new trends in this field in 2015 comparing to 2014. PMID:27620093

  2. Vocational Agriculture Education. Agricultural Mechanics.

    ERIC Educational Resources Information Center

    Smith, Eddie; And Others

    To assist teachers in agricultural mechanics in providing comprehensive instruction to their students, this curriculum guide treats both the mechanical skills and knowlege necessary for this specialized area. Six sections are included, as follow: orientation and safety; agricultural mechanics skills; agricultural power and machinery; agricultural…

  3. Continuous dry fermentation of swine manure for biogas production

    SciTech Connect

    Chen, Chuang; Zheng, Dan; Liu, Gang–Jin; Deng, Liang–Wei; Long, Yan; Fan, Zhan–Hui

    2015-04-15

    Highlights: • Continuous dry fermentation of swine manure for biogas production is feasible. • The feedstock TS concentration exerted a significant impact on biogas production. • Influences of ammonia and digestate liquidity were investigated in this study. • The results showed that the feedstock TS of swine manure should not exceed 30%. - Abstract: A down plug-flow anaerobic reactor (DPAR) was designed for the feasibility study on continuous dry fermentation of swine manure without any additional stirring. Using fresh swine manure as the feedstock with TS concentration (w/w) of 20%, 25%, 30%, and 35%, stable volumetric biogas production rates of 2.40, 1.92, 0.911, and 0.644 L·(L d){sup −1} and biogas yields of 0.665, 0.532, 0.252, and 0.178 L g{sup −1}VS were obtained respectively, and the TS degradation rates were 46.5%, 45.4%, 53.2%, and 55.6%, respectively. With the increase of feedstock TS concentration, the concentration of ammonia nitrogen grew up to the maximum value of 3500 mg L{sup −1}. Biogas production was obviously inhibited when the concentration of ammonia nitrogen was above 3000 mg L{sup −1}. The maximal volumetric biogas production rate of 2.34 L·(L d){sup −1} and biogas yield of 0.649 L g{sup −1}VS were obtained with TS concentration of 25% at 25 °C without inhibition. Liquidity experiments showed that TS concentration of digestate could be less than 15.8%, and the flow rate of digestate more than 0.98 m s{sup −1} when the feedstock TS concentration was less than 35%, which indicated the digestate could be easily discharged from a DPAR. Therefore, it is feasible to conduct a continuous dry fermentation in a DPAR using fresh swine manure as the feedstock with TS concentration less than 35%, whereas the feedstock TS concentration should not exceed 30% to achieve the maximal biogas production rate and biogas yield.

  4. The Biogas/Biofertilizer Business Handbook. Third Edition. Appropriate Technologies for Development. Reprint R-48.

    ERIC Educational Resources Information Center

    Arnott, Michael

    This book describes one approach to building and operating biogas systems. The biogas systems include raw material preparation, digesters, separate gas storage tanks, use of the gas to run engines, and the use of the sludge as fertilizer. Chapters included are: (1) "Introduction"; (2) "Biogas Systems are Small Factories"; (3) "The Raw Materials of…

  5. Energy balance model of a SOFC cogenerator operated with biogas

    NASA Astrophysics Data System (ADS)

    Van herle, Jan; Maréchal, F.; Leuenberger, S.; Favrat, D.

    A small cogeneration system based on a Solid Oxide Fuel Cell (SOFC) fed on the renewable energy source biogas is presented. An existing farm biogas production site (35 m 3 per day), currently equipped with a SOFC demonstration stack, is taken for reference. A process flow diagram was defined in a software package allowing to vary system operating parameters like the fuel inlet composition, reforming technology, stack temperature and stack current (or fuel conversion). For system reforming simplicity, a base case parameter set was defined as the fuel inlet of 60% CH 4:40% CO 2 mixed with air in a 1:1 ratio, together with 800 °C operating temperature and 80% fuel conversion. A model stack, consisting of 100 series elements of anode supported electrolyte cells of 100 cm 2 each, was calculated to deliver 3.1 kW el and 5.16 kW th from an input of 1.5 N m 3/h of biogas (8.95 kW LHV), corresponding to 33.8 and 57.6% electrical and thermal efficiencies (Lower Heating Values (LHVs)), respectively. The incidence on the efficiencies of the model system was examined by the variation of a number of parameters such as the CO 2 content in the biogas, the amount of air addition to the biogas stream, the addition of steam to the fuel inlet, the air excess ratio λ and the stack operating temperature, and the results discussed.

  6. Application of polyimide membranes for biogas purification and enrichment.

    PubMed

    Harasimowicz, M; Orluk, P; Zakrzewska-Trznadel, G; Chmielewski, A G

    2007-06-18

    Biogas is a clean environment friendly fuel that is produced by bacterial conversion of organic matter under anaerobic (oxygen-free) conditions. Raw biogas contains about 55-65% methane (CH(4)), 30-45% carbon dioxide (CO(2)), traces of hydrogen sulphide (H(2)S) and fractions of water vapour. Pure methane has a calorific value of 9100 kcal/m(3) at 15.5 degrees C and 1 atm; the calorific value of biogas varies from 4800 to 6900 kcal/m(3). To achieve the standard composition of the biogas and calorific value of 5500 kcal/m(3) the treatment techniques like absorption or membrane separation should be applied. In the paper the results of the tests of the CH(4) enrichment in simulated biogas mixture consisted of methane, carbon dioxide and hydrogen sulphide were presented. It was showed that using the capillary module with polyimide membranes it was possible to achieve the enrichment of CH(4) from the concentrations of 55-85% up to 91-94.4%. The membrane material was resistant to the small concentrations of sour gases and assured the reduction of H(2)S and water vapour concentrations, as well. The required enrichment was achieved in the single module, however to prevent CH(4) losses the multistage or hybrid systems should be used to improve process efficiency. PMID:17324508

  7. Substrate inhibition and control for high rate biogas production

    SciTech Connect

    Shin, H.S.

    1982-01-01

    This research addresses a critical aspect of the technical feasibility of biogas recovery with poultry manure using anaerobic digestion, namely, inhibition and toxicity factors limiting methane generation under high rate conditions. The research was designed to identify the limiting factors and to examine alternative pretreatment and in situ control methods for the anaerobic digestion of poultry manure as an energy producing system. Biogas production was indicated by the daily gas volume produced per unit digester capacity. Enhanced biogas generation from the anaerobic digester systems using poultry manure was studied in laboratory- and pilot-scale digester operations. It was found that ammonia nitrogen concentration above 4000 mg/l was inhibitory to biogas production. Pretreatment of the manure by elutriation was effective for decreasing inhibitory/toxic conditions. Increased gas production resulted without an indication of serious inhibition by increased volatile acids, indicating a limitation of available carbon sources. For poultry manure digestion, the optimum pH range was 7.1 to 7.6. Annual costs for pretreatment/biogas systems for 10,000, 30,000 and 50,000 birds were estimated and compared with annual surplus energy produced. The economic break-even point was achieved in digesters for greater than 30,000 birds. Capital cost of the digester system was estimated to be $18,300 with annual costs around $4000. It is anticipated that the digester system could be economically applied to smaller farms as energy costs increase.

  8. Biogas end-use in the European community

    SciTech Connect

    Constant, M.; Naveau, H.; Nyns, E.J. ); Ferrero, G.L.

    1989-01-01

    In Europe over the past few years the generation of biogas for energy and environmental purposes has been gaining in importance. Industrial wastewaters, cattle manure, sewage sludges, urban wastes, crop residues, algae and aquatic biomass are all typical of the materials being utilized. In contrast to the extensive inventory of biomethanation processes which has been carried out within the EEC, until recently a detailed, up-to-date investigation of the end-sues of biogas had not been undertaken. To supply the necessary information, the Commission of the European Communities and the Belgian Science Policy Office jointly entrusted a study to the Unit of Bioengineering at the Catholic University of Louvain, Belgium. This book is record of the study and has the following key features: it gives a broad overview of the ongoing use of biogas in Europe; it summarizes available data on storage, purification and engines using biogas; it draws several conclusions concerning the technical and economic viability of the processes; it discusses the problems of using biogas; and it outlines recommendations and future R and D and demonstration projects in the field.

  9. Increased biogas production using microbial stimulants.

    PubMed

    Singh, S; Kumar, S; Jain, M C; Kumar, D

    2001-07-01

    Laboratory studies were undertaken to evaluate the effect of microbial stimulants Aquasan and Teresan, on biogas yields from cattle dung and combined residues of cattle dung and kitchen waste, respectively. The addition of single dose of Aquasan at the rate of 10, 15 and 20 ppm to cattle dung on the first day of incubation resulted in increased gas yields ranging between 45.1 and 62.1 l/kg dry matter. Subsequent addition of Aquasan at 15 and 20 ppm dosage after a period of 15 days increased the gas yields by 15-16%. The gas production was found to be optimum at a dosage level of 15 ppm and was 39% and 55% higher with single and dual additions, respectively, than untreated cattle dung. In another bench scale study (1:1 dry matter) the addition of Teresan at 10 ppm concentration to the mixed residues of cattle dung and kitchen wastes at different solids concentration, produced 34.8% more gas (272.4 l/kg d.m.) than the uninoculated mixture at 15% TS concentration (202.4 l/kg d.m.). PMID:11341694

  10. Poisonous Plants

    MedlinePlus

    ... Publications and Products Programs Contact NIOSH NIOSH POISONOUS PLANTS Recommend on Facebook Tweet Share Compartir Photo courtesy ... U.S. Department of Agriculture Many native and exotic plants are poisonous to humans when ingested or if ...

  11. Biogas desulfurization and biogas upgrading using a hybrid membrane system--modeling study.

    PubMed

    Makaruk, A; Miltner, M; Harasek, M

    2013-01-01

    Membrane gas permeation using glassy membranes proved to be a suitable method for biogas upgrading and natural gas substitute production on account of low energy consumption and high compactness. Glassy membranes are very effective in the separation of bulk carbon dioxide and water from a methane-containing stream. However, the content of hydrogen sulfide can be lowered only partially. This work employs process modeling based upon the finite difference method to evaluate a hybrid membrane system built of a combination of rubbery and glassy membranes. The former are responsible for the separation of hydrogen sulfide and the latter separate carbon dioxide to produce standard-conform natural gas substitute. The evaluation focuses on the most critical upgrading parameters like achievable gas purity, methane recovery and specific energy consumption. The obtained results indicate that the evaluated hybrid membrane configuration is a potentially efficient system for the biogas processing tasks that do not require high methane recoveries, and allows effective desulfurization for medium and high hydrogen sulfide concentrations without additional process steps. PMID:23168631

  12. Remote sensing in Virginia agriculture

    NASA Technical Reports Server (NTRS)

    Pettry, D. E.; Newhouse, M. E.; Dunton, E. M., Jr.; Scott, J. H., Jr.

    1972-01-01

    An experimental investigation, designed to develop and evaluate multispectral sensing techniques used in sensing agricultural crops, is described. Initial studies were designed to detect plant species and associated diseases, soil variations, and cultural practices under natural environment conditions. In addition, crop varieties, age, spacing, plant height, percentage of ground cover, and plant vigor are determined.

  13. Mechanisms of cementitious material deterioration in biogas digester.

    PubMed

    Voegel, C; Bertron, A; Erable, B

    2016-11-15

    Digesters produce biogas from organic wastes through anaerobic digestion processes. These digesters, often made of concrete, suffer severe premature deterioration caused mainly by the presence of fermentative microorganisms producing metabolites that are aggressive towards cementitious materials. To clarify the degradation mechanisms in an anaerobic digestion medium, ordinary Portland cement paste specimens were immersed in the liquid fraction of a running, lab-scale digester for 4weeks. The anaerobic digestion medium was a mixture of a biowaste substrate and sludge from municipal wastewater treatment plant used as a source of anaerobic bacteria. The chemical characteristics of the anaerobic digestion liquid phase were monitored over time using a pH metre, high performance liquid chromatography (HPLC) and ion chromatography (HPIC). An initial critical period of low pH in the bioreactors was observed before the pH stabilized around 8. Acetic, propionic and butyric acids were produced during the digestion with a maximum total organic acid concentration of 50mmolL(-1). The maximum ammonium content of the liquid phase was 40mmolL(-1), which was about seven times the upper limit of the highly aggressive chemical environment class (XA3) as defined by the European standard for the specification of concrete design in chemically aggressive environments (EN 206). The changes in the mineralogical, microstructural and chemical characteristics of the cement pastes exposed to the solid and liquid phase of the digesters were analysed at the end of the immersion period by X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectrometry (EDS) and electron-probe micro-analysis (EPMA). A 700-μm thick altered layer was identified in the cement paste specimens. The main biodeterioration patterns in the bioreactors' solid/liquid phase were calcium leaching and carbonation of the cement matrix. PMID:27432729

  14. Genetic and Symbiotic Diversity of Nitrogen-Fixing Bacteria Isolated from Agricultural Soils in the Western Amazon by Using Cowpea as the Trap Plant

    PubMed Central

    Azarias Guimarães, Amanda; Duque Jaramillo, Paula Marcela; Simão Abrahão Nóbrega, Rafaela; Florentino, Ligiane Aparecida; Barroso Silva, Karina

    2012-01-01

    Cowpea is a legume of great agronomic importance that establishes symbiotic relationships with nitrogen-fixing bacteria. However, little is known about the genetic and symbiotic diversity of these bacteria in distinct ecosystems. Our study evaluated the genetic diversity and symbiotic efficiencies of 119 bacterial strains isolated from agriculture soils in the western Amazon using cowpea as a trap plant. These strains were clustered into 11 cultural groups according to growth rate and pH. The 57 nonnodulating strains were predominantly fast growing and acidifying, indicating a high incidence of endophytic strains in the nodules. The other 62 strains, authenticated as nodulating bacteria, exhibited various symbiotic efficiencies, with 68% of strains promoting a significant increase in shoot dry matter of cowpea compared with the control with no inoculation and low levels of mineral nitrogen. Fifty genotypes with 70% similarity and 21 genotypes with 30% similarity were obtained through repetitive DNA sequence (BOX element)-based PCR (BOX-PCR) clustering. The 16S rRNA gene sequencing of strains representative of BOX-PCR clusters showed a predominance of bacteria from the genus Bradyrhizobium but with high species diversity. Rhizobium, Burkholderia, and Achromobacter species were also identified. These results support observations of cowpea promiscuity and demonstrate the high symbiotic and genetic diversity of rhizobia species in areas under cultivation in the western Amazon. PMID:22798370

  15. Factors Affecting Process Temperature and Biogas Production in Small-scale Rural Biogas Digesters in Winter in Northern Vietnam.

    PubMed

    Pham, C H; Vu, C C; Sommer, S G; Bruun, S

    2014-07-01

    This study investigated the main factors influencing digester temperature and methods to reduce heat losses during the cold season in the subtropics. Four composite digesters (two insulated and two uninsulated) were buried underground to measure their internal temperature (°C) at a depth of 140 cm and 180 cm, biogas production and methane (CH4) concentration in biogas from August to February. In parallel the temperature of the air (100 cm above ground), in the slurry mixing tank and in the soil (10, 100, 140, and 180 cm depth) was measured by thermocouple. The influent amount was measured daily and the influent chemical composition was measured monthly during the whole experimental period. Seasonal variations in air temperature significantly affected the temperature in the soil, mixing tank and digester. Consequently, biogas production, which is temperature dependent, was influenced by the season. The main factors determining the internal temperature in the digesters were insulation with Styrofoam, air temperature and temperature of slurry in the mixing tank. Biogas production is low due to the cold climate conditions in winter in Northern Vietnam, but the study proved that storing slurry in the mixing tank until its temperature peak at around 14:00 h will increase the temperature in the digester and thus increase potential biogas production. Algorithms are provided linking digester temperature to the temperature of slurry in the mixing tank. PMID:25050049

  16. Factors Affecting Process Temperature and Biogas Production in Small-scale Rural Biogas Digesters in Winter in Northern Vietnam

    PubMed Central

    Pham, C. H.; Vu, C. C.; Sommer, S. G.; Bruun, S.

    2014-01-01

    This study investigated the main factors influencing digester temperature and methods to reduce heat losses during the cold season in the subtropics. Four composite digesters (two insulated and two uninsulated) were buried underground to measure their internal temperature (°C) at a depth of 140 cm and 180 cm, biogas production and methane (CH4) concentration in biogas from August to February. In parallel the temperature of the air (100 cm above ground), in the slurry mixing tank and in the soil (10, 100, 140, and 180 cm depth) was measured by thermocouple. The influent amount was measured daily and the influent chemical composition was measured monthly during the whole experimental period. Seasonal variations in air temperature significantly affected the temperature in the soil, mixing tank and digester. Consequently, biogas production, which is temperature dependent, was influenced by the season. The main factors determining the internal temperature in the digesters were insulation with Styrofoam, air temperature and temperature of slurry in the mixing tank. Biogas production is low due to the cold climate conditions in winter in Northern Vietnam, but the study proved that storing slurry in the mixing tank until its temperature peak at around 14:00 h will increase the temperature in the digester and thus increase potential biogas production. Algorithms are provided linking digester temperature to the temperature of slurry in the mixing tank. PMID:25050049

  17. Microalgal Cultivation in Treating Liquid Digestate from Biogas Systems.

    PubMed

    Xia, Ao; Murphy, Jerry D

    2016-04-01

    Biogas production via anaerobic digestion (AD) has rapidly developed in recent years. In addition to biogas, digestate is an important byproduct. Liquid digestate is the major fraction of digestate and may contain high levels of ammonia nitrogen. Traditional processing technologies (such as land application) require significant energy inputs and raise environmental risks (such as eutrophication). Alternatively, microalgae can efficiently remove the nutrients from digestate while producing high-value biomass that can be used for the production of biochemicals and biofuels. Both inorganic and organic carbon sources derived from biogas production can significantly improve microalgal production. Land requirement for microalgal cultivation is estimated as 3% of traditional direct land application of digestate. PMID:26776247

  18. Agriculture Education. Horticulture.

    ERIC Educational Resources Information Center

    Stuttgart Public Schools, AR.

    This curriculum guide is designed for group instruction of secondary agricultural education students enrolled in one or two semester-long courses in ornamental horticulture. The guide presents units of study in the following areas: (1) horticulture and job opportunities, (2) preparing soil mixtures, (3) control, (4) plant propagation, (5) plant…

  19. Agriculture, summary

    NASA Technical Reports Server (NTRS)

    Baldwin, R.

    1975-01-01

    Applications of remotely sensed data in agriculture are enumerated. These include: predictions of forage for range animal consumption, forest management, soil mapping, and crop inventory and management.

  20. Increasing biogas production from sewage sludge anaerobic co-digestion process by adding crude glycerol from biodiesel industry.

    PubMed

    Nartker, Steven; Ammerman, Michelle; Aurandt, Jennifer; Stogsdil, Michael; Hayden, Olivia; Antle, Chad

    2014-12-01

    In an effort to convert waste streams to energy in a green process, glycerol from biodiesel manufacturing has been used to increase the gas production and methane content of biogas within a mesophilic anaerobic co-digestion process using primary sewage sludge. Glycerol was systematically added to the primary digester from 0% to 60% of the organic loading rate (OLR). The optimum glycerol loading range was from 25% to 60% OLR. This resulted in an 82-280% improvement in specific gas production. Following the feeding schedule described, the digesters remained balanced and healthy until inhibition was achieved at 70% glycerol OLR. This suggests that high glycerol loadings are possible if slow additions are upheld in order to allow the bacterial community to adjust properly. Waste water treatment plant operators with anaerobic digesters can use the data to increase loadings and boost biogas production to enhance energy conversion. This process provides a safe, environmentally friendly method to convert a typical waste stream to an energy stream of biogas. PMID:25249492

  1. Improving the mixing performances of rice straw anaerobic digestion for higher biogas production by computational fluid dynamics (CFD) simulation.

    PubMed

    Shen, Fei; Tian, Libin; Yuan, Hairong; Pang, Yunzhi; Chen, Shulin; Zou, Dexun; Zhu, Baoning; Liu, Yanping; Li, Xiujin

    2013-10-01

    As a lignocellulose-based substrate for anaerobic digestion, rice straw is characterized by low density, high water absorbability, and poor fluidity. Its mixing performances in digestion are completely different from traditional substrates such as animal manures. Computational fluid dynamics (CFD) simulation was employed to investigate mixing performances and determine suitable stirring parameters for efficient biogas production from rice straw. The results from CFD simulation were applied in the anaerobic digestion tests to further investigate their reliability. The results indicated that the mixing performances could be improved by triple impellers with pitched blade, and complete mixing was easily achieved at the stirring rate of 80 rpm, as compared to 20-60 rpm. However, mixing could not be significantly improved when the stirring rate was further increased from 80 to 160 rpm. The simulation results agreed well with the experimental results. The determined mixing parameters could achieve the highest biogas yield of 370 mL (g TS)(-1) (729 mL (g TS(digested))(-1)) and 431 mL (g TS)(-1) (632 mL (g TS(digested))(-1)) with the shortest technical digestion time (T 80) of 46 days. The results obtained in this work could provide useful guides for the design and operation of biogas plants using rice straw as substrates. PMID:23873639

  2. Effect of oxytetracycline on biogas production and active microbial populations during batch anaerobic digestion of cow manure.

    PubMed

    Ince, Bahar; Coban, Halil; Turker, Gokhan; Ertekin, Emine; Ince, Orhan

    2013-05-01

    The aim of this study was to investigate the effect of a common veterinary antibiotic in biogas plants. 20 mg/kg of oxytetracycline was intramuscularly injected into a cow and its concentration in manure, which was sampled daily during the following 20 days, was measured. A total of 20 % of the injected oxytetracycline was detected in manure. Collected manure samples on days 1, 2, 3, 5, 10, 15, and 20 were digested in triplicate serum bottles at 37 °C for 30 days. Control serum bottles produced 255 ± 13 mL biogas, whereas 50-60 % inhibitions were obtained for the serum bottles operated with samples collected for the 5 days after medication. Multivariate statistics used for the evaluation of FISH results showed that Methanomicrobiales were the main methanogenic group responsible for most of the biogas production. Numbers of active Bacteria and Methanomicrobiales were negatively correlated with the presence of oxytetracycline, whereas Methanosarcinales and Methanobacteriales were less affected. PMID:22903595

  3. Agricultural Production. An Administrative Guide for Agricultural Education.

    ERIC Educational Resources Information Center

    Henrico County Public Schools, Glen Allen, VA. Virginia Vocational Curriculum Center.

    This basic instructional guide for an agricultural production program is one in a series of such guides for agricultural education. It is useful in developing and selecting instructional material and implementing competency-based education for a program directed toward helping students to become proficient in animal, plant, and soil sciences and…

  4. Novel anaerobic digestion process with sludge ozonation for economically feasible power production from biogas.

    PubMed

    Komatsu, K; Yasui, H; Goel, R; Li, Y Y; Noike, T

    2011-01-01

    A novel process scheme was developed to achieve economically feasible energy recovery from anaerobic digestion. The new process scheme employs a hybrid configuration of mesophilic and thermophilic anaerobic digestion with sludge ozonation: the ozonated sludge is first degraded in a thermophilic digester and then further degraded in a mesophilic digester. In small-scale pilot experiments of the new process scheme, degradation of VSS improved by 3.5% over the control (mesophilic-only configuration) with 20% less ozone consumption. Moreover, biogas conversion also improved by 7.1% over the control. Selective enrichment of inorganic compounds during centrifugation produced a dewatered sludge cake with very low water content (59.4%). This low water content in the sludge cake improved its auto-thermal combustion potential during incineration and added to the overall energy savings. We conducted a case study to evaluate power generation from biogas for a municipal wastewater treatment plant with an average dry weather flow of 43,000 m3/d. Electricity production cost was 5.2 ¢/kWh for the advanced process with power generation, which is lower than the current market price of 7.2 ¢/kWh. The new anaerobic digestion scheme with power generation may reduce greenhouse gas emissions by about 1,000 t-CO(2)/year compared with the conventional process without power generation. PMID:21508552

  5. Biotechnological Screening of Microalgal and Cyanobacterial Strains for Biogas Production and Antibacterial and Antifungal Effects

    PubMed Central

    Mudimu, Opayi; Rybalka, Nataliya; Bauersachs, Thorsten; Born, Jens; Friedl, Thomas; Schulz, Rüdiger

    2014-01-01

    Microalgae and cyanobacteria represent a valuable natural resource for the generation of a large variety of chemical substances that are of interest for medical research, can be used as additives in cosmetics and food production, or as an energy source in biogas plants. The variety of potential agents and the use of microalgae and cyanobacteria biomass for the production of these substances are little investigated and not exploited for the market. Due to the enormous biodiversity of microalgae and cyanobacteria, they hold great promise for novel products. In this study, we investigated a large number of microalgal and cyanobacterial strains from the Culture Collection of Algae at Göttingen University (SAG) with regard to their biomass and biogas production, as well antibacterial and antifungal effects. Our results demonstrated that microalgae and cyanobacteria are able to generate a large number of economically-interesting substances in different quantities dependent on strain type. The distribution and quantity of some of these components were found to reflect phylogenetic relationships at the level of classes. In addition, between closely related species and even among multiple isolates of the same species, the productivity may be rather variable. PMID:24957031

  6. Utilization of pentoses from sugarcane biomass: techno-economics of biogas vs. butanol production.

    PubMed

    Mariano, Adriano Pinto; Dias, Marina O S; Junqueira, Tassia L; Cunha, Marcelo P; Bonomi, Antonio; Filho, Rubens Maciel

    2013-08-01

    This paper presents the techno-economics of greenfield projects of an integrated first and second-generation sugarcane biorefinery in which pentose sugars obtained from sugarcane biomass are used either for biogas (consumed internally in the power boiler) or n-butanol production via the ABE batch fermentation process. The complete sugarcane biorefinery was simulated using Aspen Plus®. Although the pentoses stream available in the sugarcane biorefinery gives room for a relatively small biobutanol plant (7.1-12 thousand tonnes per year), the introduction of butanol and acetone to the product portfolio of the biorefinery increased and diversified its revenues. Whereas the IRR of the investment on a biorefinery with biogas production is 11.3%, IRR varied between 13.1% and 15.2% in the butanol production option, depending on technology (regular or engineered microorganism with improved butanol yield and pentoses conversion) and target market (chemicals or automotive fuels). Additional discussions include the effects of energy-efficient technologies for butanol processing on the profitability of the biorefinery. PMID:23748087

  7. Maximising biogas in anaerobic digestion by using engine waste heat for thermal hydrolysis pre-treatment of sludge.

    PubMed

    Pickworth, B; Adams, J; Panter, K; Solheim, O E

    2006-01-01

    Dublin's Ringsend WWTP was designed to serve a population of approximately 1.2 million p.e. with a sludge production of 37,000 dry tonnes per year after upgrading to full secondary treatment. Several technical solutions were put forward as part of a design, build, finance and operate (DBFO) competition, with the chosen solution being a proposal by Black and Veatch for a combination of sequencing batch reactor (SBR) technology and anaerobic digestion with Cambi thermal hydrolysis pre-treatment (THP). The THP plant was built by Cambi and handed over to B&V in 2002. The plant is now operated by Celtic Anglian Water. In September 2004 a test was carried out on the mass and energy balance of the plant following 2 years of operation and is detailed in this paper. The process enables digestion at very high dry solids feed and low hydraulic retention time. The plant was built with three digesters of 4250 m3 each and is fed with hydrolysed sludge at 11% DS. There are four no. 1 MW Jenbacher engines operating mainly on biogas. Each pair of engines is fitted with a waste heat boiler with a capacity of one tonne steam per hour. These boilers have sufficient capacity to provide 80% of the steam required for the THP, which in turn provides all the heat for the subsequent digestion in the form of hydrolysed feed. There are two main biogas boilers for top up steam and other uses of the biogas including thermal oxidation of concentrated odours. PMID:17087375

  8. A review on optimization production and upgrading biogas through CO2 removal using various techniques.

    PubMed

    Andriani, Dian; Wresta, Arini; Atmaja, Tinton Dwi; Saepudin, Aep

    2014-02-01

    Biogas from anaerobic digestion of organic materials is a renewable energy resource that consists mainly of CH4 and CO2. Trace components that are often present in biogas are water vapor, hydrogen sulfide, siloxanes, hydrocarbons, ammonia, oxygen, carbon monoxide, and nitrogen. Considering the biogas is a clean and renewable form of energy that could well substitute the conventional source of energy (fossil fuels), the optimization of this type of energy becomes substantial. Various optimization techniques in biogas production process had been developed, including pretreatment, biotechnological approaches, co-digestion as well as the use of serial digester. For some application, the certain purity degree of biogas is needed. The presence of CO2 and other trace components in biogas could affect engine performance adversely. Reducing CO2 content will significantly upgrade the quality of biogas and enhancing the calorific value. Upgrading is generally performed in order to meet the standards for use as vehicle fuel or for injection in the natural gas grid. Different methods for biogas upgrading are used. They differ in functioning, the necessary quality conditions of the incoming gas, and the efficiency. Biogas can be purified from CO2 using pressure swing adsorption, membrane separation, physical or chemical CO2 absorption. This paper reviews the various techniques, which could be used to optimize the biogas production as well as to upgrade the biogas quality. PMID:24293277

  9. Molecular tools used in agriculture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A summary of molecular tools used for research in agriculture were presented. Examples of DNA sequencing, library preparation, use of fingerprinting for pathogens and plant crops, high throughput sequencing, whole-genome amplification, reporter genes, and other methods....

  10. Agricultural Wastes.

    ERIC Educational Resources Information Center

    Jewell, W. J.; Switzenbaum, M. S.

    1978-01-01

    Presents a literature review of agricultural wastes, covering publications of 1976-77. Some of the areas covered are: (1) water characteristics and impacts; (2) waste treatment; (3) reuse of agricultural wastes; and (4) nonpoint pollution sources. A list of 150 references is also presented. (HM)

  11. VOCATIONAL AGRICULTURE.

    ERIC Educational Resources Information Center

    California State Dept. of Education, Sacramento. Research Coordinating Unit.

    TO ASSIST THOSE WHO MAKE DECISIONS RELATING TO EDUCATIONAL PROGRAMS IN AGRICULTURE, RECENT RESEARCH IN VOCATIONAL AGRICULTURE IS SUMMARIZED. A 1963 STUDY TREATS THE RELATIONSHIP BETWEEN WORK EXPERIENCE AND STUDENT CHARACTERISTICS, PLANS, AND ASPIRATIONS. STUDIES ON POST-SECONDARY EDUCATION CONCERN GUIDELINES FOR TECHNICIAN PROGRAMS, JUSTIFICATION…

  12. Seasonal dynamics in photosynthesis of woody plants at the northern limit of Asian tropics: potential role of fog in maintaining tropical rainforests and agriculture in Southwest China.

    PubMed

    Zhang, Yong-Jiang; Holbrook, N Michele; Cao, Kun-Fang

    2014-10-01

    The lowland tropical rainforests in Xishuangbanna, Southwest (SW) China, mark the northern limit of Asian tropics. Fog has been hypothesized to play a role in maintaining rainforests and tropical crop production in this region, but the physiological mechanism has not been studied. The goals of this study were to characterize the seasonal dynamics in photosynthesis and to assess the potential for fog to mitigate chilling-induced photodamage for tropical trees and crops in Xishuangbanna. We measured seasonal dynamics in light-saturated net photosynthetic rate (Aa), stomatal conductance (gs), intercellular CO2 concentration, quantum yield of Photosystem II (Fv/Fm) and maximum P700 changes (Pm; indicates the amount of active PSI complex), as well as chilling resistance and fog (light/shading) effects on low temperature-induced decline in Fv/Fm and Pm for native tree and introduced lower latitude tree or woody shrub species grown in a tropical botanical garden. Despite significant decreases in Aa, gs, Pm and Fv/Fm, most species maintained considerably high Aa during the cool season (2.51-14.6 μmol m(-2) s(-1)). Shaded leaves exposed to seasonal low temperatures had higher Fv/Fm than sun-exposed leaves in the cool season. All species could tolerate 1.4 °C in the dark, whereas a combined treatment of low temperature and high light caused a distinctly faster decline in Pm and Fv/Fm compared with low temperature treatment alone. Because fog persistence avoids or shortens the duration of high light condition in the morning when the temperatures are still low, our results provide support for the hypothesis that fog reduces chilling damage to tropical plants in this region and thus plays a role in maintaining tropical rainforests and agriculture in SW China. PMID:25298374

  13. Potential use of the facultative halophyte Chenopodium quinoa Willd. as substrate for biogas production cultivated with different concentrations of sodium chloride under hydroponic conditions.

    PubMed

    Turcios, Ariel E; Weichgrebe, Dirk; Papenbrock, Jutta

    2016-03-01

    This project analyses the biogas potential of the halophyte Chenopodium quinoa Willd. In a first approach C. quinoa was grown with different concentrations of NaCl (0, 10 and 20 ppt NaCl) and the crop residues were used as substrate for biogas production. In a second approach, C. quinoa was grown with 0, 10, 20 and 30 ppt NaCl under hydroponic conditions and the fresh biomass was used as substrate. The more NaCl is in the culture medium, the higher the sodium, potassium, crude ash and hemicellulose content in the plant tissue whereas the calcium, sulfur, nitrogen and carbon content in the biomass decrease. According to this study, it is possible to produce high yields of methane using biomass of C. quinoa. The highest specific methane yields were obtained using the substrate from the plants cultivated at 10 and 20 ppt NaCl in both experiments. PMID:26744800

  14. Biomass-to-electricity: analysis and optimization of the complete pathway steam explosion--enzymatic hydrolysis--anaerobic digestion with ICE vs SOFC as biogas users.

    PubMed

    Santarelli, M; Barra, S; Sagnelli, F; Zitella, P

    2012-11-01

    The paper deals with the energy analysis and optimization of a complete biomass-to-electricity energy pathway, starting from raw biomass towards the production of renewable electricity. The first step (biomass-to-biogas) is based on a real pilot plant located in Environment Park S.p.A. (Torino, Italy) with three main steps ((1) impregnation; (2) steam explosion; (3) enzymatic hydrolysis), completed by a two-step anaerobic fermentation. In the second step (biogas-to-electricity), the paper considers two technologies: internal combustion engines and a stack of solid oxide fuel cells. First, the complete pathway has been modeled and validated through experimental data. After, the model has been used for an analysis and optimization of the complete thermo-chemical and biological process, with the objective function of maximization of the energy balance at minimum consumption. The comparison between ICE and SOFC shows the better performance of the integrated plants based on SOFC. PMID:22940353

  15. Biogas generation in anaerobic wastewater treatment under tetracycline antibiotic pressure

    PubMed Central

    Lu, Meiqing; Niu, Xiaojun; Liu, Wei; Zhang, Jun; Wang, Jie; Yang, Jia; Wang, Wenqi; Yang, Zhiquan

    2016-01-01

    The effect of tetracycline (TC) antibiotic on biogas generation in anaerobic wastewater treatment was studied. A lab-scale Anaerobic Baffled Reactor (ABR) with three compartments was used. The reactor was operated with synthetic wastewater in the absence of TC and in the presence of 250 μg/L TC for 90 days, respectively. The removal rate of TC, volatile fatty acids (VFAs), biogas compositions (hydrogen (H2), methane (CH4), carbon dioxide (CO2)), and total biogas production in each compartment were monitored in the two operational conditions. Results showed that the removal rate of TC was 14.97–67.97% in the reactor. The presence of TC had a large negative effect on CH4 and CO2 generation, but appeared to have a positive effect on H2 production and VFAs accumulation. This response indicated that the methanogenesis process was sensitive to TC presence, but the acidogenesis process was insensitive. This suggested that the presence of TC had less influence on the degradation of organic matter but had a strong influence on biogas generation. Additionally, the decrease of CH4 and CO2 generation and the increase of H2 and VFAs accumulation suggest a promising strategy to help alleviate global warming and improve resource recovery in an environmentally friendly approach. PMID:27341657

  16. Biogas generation in anaerobic wastewater treatment under tetracycline antibiotic pressure.

    PubMed

    Lu, Meiqing; Niu, Xiaojun; Liu, Wei; Zhang, Jun; Wang, Jie; Yang, Jia; Wang, Wenqi; Yang, Zhiquan

    2016-01-01

    The effect of tetracycline (TC) antibiotic on biogas generation in anaerobic wastewater treatment was studied. A lab-scale Anaerobic Baffled Reactor (ABR) with three compartments was used. The reactor was operated with synthetic wastewater in the absence of TC and in the presence of 250 μg/L TC for 90 days, respectively. The removal rate of TC, volatile fatty acids (VFAs), biogas compositions (hydrogen (H2), methane (CH4), carbon dioxide (CO2)), and total biogas production in each compartment were monitored in the two operational conditions. Results showed that the removal rate of TC was 14.97-67.97% in the reactor. The presence of TC had a large negative effect on CH4 and CO2 generation, but appeared to have a positive effect on H2 production and VFAs accumulation. This response indicated that the methanogenesis process was sensitive to TC presence, but the acidogenesis process was insensitive. This suggested that the presence of TC had less influence on the degradation of organic matter but had a strong influence on biogas generation. Additionally, the decrease of CH4 and CO2 generation and the increase of H2 and VFAs accumulation suggest a promising strategy to help alleviate global warming and improve resource recovery in an environmentally friendly approach. PMID:27341657

  17. Biogas energy production from tropical biomass wastes by anaerobic digestion

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Anaerobic digestion (AD) is an attractive technology in tropical regions for converting locally abundant biomass wastes into biogas which can be used to produce heat, electricity, and transportation fuels. However, investigations on AD of tropical forestry wastes, such as albizia biomass, and food w...

  18. Air Quality, Climate and Economic Impacts of Biogas Management Technologies

    EPA Science Inventory

    Anaerobically digested organic waste (e.g. manure, sewage, and municipal solid waste) produces biogas, a source of renewable energy. A recent analysis indicates that the technical resource in California could produce nearly 93 billion cubic feet per year of biomethane from availa...

  19. Optimization of biogas production from coffee production waste.

    PubMed

    Battista, Federico; Fino, Debora; Mancini, Giuseppe

    2016-01-01

    This study was conducted to investigate the effects of chemical pretreatments on biogas production from coffee waste. After the preparation of a mixture of coffee waste with a TS concentration of 10%w/w, basic and acid pretreatments were conducted in batch mode and their performances were compared with the biogas produced from a mixture without any pretreatment stage. The basic pretreatment demonstrated a very good action on the hydrolysis of the lignin and cellulose, and permitted a biogas production of about 18NL/L with a methane content of almost 80%v/v. Thus, the basic pretreatment has been used to scale-up the process. The coffee refuse was has been carried out in a 45L anaerobic reactor working in continuous mode and in a mesophilic condition (35°C) with a Hydraulic Retention Time (HRT) of about 40days. A high biogas production of 1.14NL/Ld, with a methane percentage of 65%v/v was obtained, thus permitting a process yield of about 83% to be obtained. PMID:26600457

  20. Biogas generation in anaerobic wastewater treatment under tetracycline antibiotic pressure

    NASA Astrophysics Data System (ADS)

    Lu, Meiqing; Niu, Xiaojun; Liu, Wei; Zhang, Jun; Wang, Jie; Yang, Jia; Wang, Wenqi; Yang, Zhiquan

    2016-06-01

    The effect of tetracycline (TC) antibiotic on biogas generation in anaerobic wastewater treatment was studied. A lab-scale Anaerobic Baffled Reactor (ABR) with three compartments was used. The reactor was operated with synthetic wastewater in the absence of TC and in the presence of 250 μg/L TC for 90 days, respectively. The removal rate of TC, volatile fatty acids (VFAs), biogas compositions (hydrogen (H2), methane (CH4), carbon dioxide (CO2)), and total biogas production in each compartment were monitored in the two operational conditions. Results showed that the removal rate of TC was 14.97–67.97% in the reactor. The presence of TC had a large negative effect on CH4 and CO2 generation, but appeared to have a positive effect on H2 production and VFAs accumulation. This response indicated that the methanogenesis process was sensitive to TC presence, but the acidogenesis process was insensitive. This suggested that the presence of TC had less influence on the degradation of organic matter but had a strong influence on biogas generation. Additionally, the decrease of CH4 and CO2 generation and the increase of H2 and VFAs accumulation suggest a promising strategy to help alleviate global warming and improve resource recovery in an environmentally friendly approach.

  1. Complete Genome Sequence of Herbinix luporum SD1D, a New Cellulose-Degrading Bacterium Isolated from a Thermophilic Biogas Reactor

    PubMed Central

    Koeck, Daniela E.; Maus, Irena; Wibberg, Daniel; Winkler, Anika; Zverlov, Vladimir V.; Liebl, Wolfgang; Pühler, Alfred; Schwarz, Wolfgang H.

    2016-01-01

    A novel cellulolytic bacterial strain was isolated from an industrial-scale biogas plant. The 16S rRNA gene sequence of the strain SD1D showed 96.4% similarity to Herbinix hemicellulosilytica T3/55T, indicating a novel species within the genus Herbinix (family Lachnospiraceae). Here, the complete genome sequence of Herbinix luporum SD1D is reported. PMID:27445379

  2. Complete Genome Sequence of Herbinix luporum SD1D, a New Cellulose-Degrading Bacterium Isolated from a Thermophilic Biogas Reactor.

    PubMed

    Koeck, Daniela E; Maus, Irena; Wibberg, Daniel; Winkler, Anika; Zverlov, Vladimir V; Liebl, Wolfgang; Pühler, Alfred; Schwarz, Wolfgang H; Schlüter, Andreas

    2016-01-01

    A novel cellulolytic bacterial strain was isolated from an industrial-scale biogas plant. The 16S rRNA gene sequence of the strain SD1D showed 96.4% similarity to Herbinix hemicellulosilytica T3/55(T), indicating a novel species within the genus Herbinix (family Lachnospiraceae). Here, the complete genome sequence of Herbinix luporum SD1D is reported. PMID:27445379

  3. Draft Genome Sequence of Propionispora sp. Strain 2/2-37, a New Xylan-Degrading Bacterium Isolated from a Mesophilic Biogas Reactor

    PubMed Central

    Koeck, Daniela E.; Maus, Irena; Wibberg, Daniel; Winkler, Anika; Zverlov, Vladimir V.; Liebl, Wolfgang; Pühler, Alfred; Schwarz, Wolfgang H.

    2016-01-01

    The novel mesophilic bacterial strain Propionispora sp. 2/2-37 was isolated from an industrial-scale biogas plant. Comparative 16S rRNA gene sequencing revealed that the isolate constitutes a new subcluster within the order Selenomonadales. The 2/2-37 draft genome sequence was established and provides the genetic basis for application of this microorganism in degradation of biomass for bio-fuel production. PMID:27340074

  4. Agricultural Geophysics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The four geophysical methods predominantly used for agricultural purposes are resistivity, electromagnetic induction, ground penetrating radar (GPR), and time domain reflectometry (TDR). Resistivity and electromagnetic induction methods are typically employed to map lateral variations of apparent so...

  5. Biological pre-treatment: Enhancing biogas production using the highly cellulolytic fungus Trichoderma viride.

    PubMed

    Mutschlechner, Mira; Illmer, Paul; Wagner, Andreas Otto

    2015-09-01

    With regard to renewable sources of energy, bioconversion of lignocellulosic biomass has long been recognized as a desirable endeavor. However, the highly heterogeneous structure of lignocellulose restricts the exploitation of its promising potential in biogas plants. Hence, effective pre-treatment methods are decisive prerequisites to overcome these challenges in order to improve the utilization ratio of (ligno) cellulosic substrates during fermentation. In the present study, the application of Trichoderma viride in an aerobic upstream process prior to anaerobic digestion led up to a threefold increase in the yield of methane and total gas in a lab-scale investigation. Due to its highly efficient cellulolytic activities, T. viride seemed to be responsible for an improved nutrient availability that positively influenced the anaerobic microbiocenosis. Aerobic pre-treatment of organic matter with T. viride is therefore a promising solution to achieve higher methane yields and degradation performances without any additional energy demand, nor undesired by-product inhibition. PMID:26013693

  6. Phosphorus recovery from biogas fermentation liquid by Ca-Mg loaded biochar.

    PubMed

    Fang, Ci; Zhang, Tao; Li, Ping; Jiang, Rongfeng; Wu, Shubiao; Nie, Haiyu; Wang, Yingcai

    2015-03-01

    Shortage in phosphorus (P) resources and P wastewater pollution is considered as a serious problem worldwide. The application of modified biochar for P recovery from wastewater and reuse of recovered P as agricultural fertilizer is a preferred process. This work aims to develop a calcium and magnesium loaded biochar (Ca-Mg/biochar) application for P recovery from biogas fermentation liquid. The physico-chemical characterization, adsorption efficiency, adsorption selectivity, and postsorption availability of Ca-Mg/biochar were investigated. The synthesized Ca-Mg/biochar was rich in organic functional groups and in CaO and MgO nanoparticles. With the increase in synthesis temperature, the yield decreased, C content increased, H content decreased, N content remained the same basically, and BET surface area increased. The P adsorption of Ca-Mg/biochar could be accelerated by nano-CaO and nano-MgO particles and reached equilibrium after 360min. The process was endothermic, spontaneous, and showed an increase in the disorder of the solid-liquid interface. Moreover, it could be fitted by the Freundlich model. The maximum P adsorption amounts were 294.22, 315.33, and 326.63mg/g. The P adsorption selectivity of Ca-Mg/biochar could not be significantly influenced by the typical pH level of biogas fermentation liquid. The nano-CaO and nano-MgO particles of Ca-Mg/biochar could reduce the negative interaction effects of coexisting ions. The P releasing amounts of postsorption Ca-Mg/biochar were in the order of Ca-Mg/B600>Ca-Mg/B450>Ca-Mg/B300. Results revealed that postsorption Ca-Mg/biochar can continually release P and is more suitable for an acid environment. PMID:25766018

  7. 7 CFR 302.2 - Movement of plants and plant products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Movement of plants and plant products. 302.2 Section 302.2 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE DISTRICT OF COLUMBIA; MOVEMENT OF PLANTS AND PLANT...

  8. 7 CFR 302.2 - Movement of plants and plant products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 5 2013-01-01 2013-01-01 false Movement of plants and plant products. 302.2 Section 302.2 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE DISTRICT OF COLUMBIA; MOVEMENT OF PLANTS AND PLANT...

  9. 7 CFR 302.2 - Movement of plants and plant products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 5 2011-01-01 2011-01-01 false Movement of plants and plant products. 302.2 Section 302.2 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE DISTRICT OF COLUMBIA; MOVEMENT OF PLANTS AND PLANT...

  10. 7 CFR 302.2 - Movement of plants and plant products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 5 2012-01-01 2012-01-01 false Movement of plants and plant products. 302.2 Section 302.2 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE DISTRICT OF COLUMBIA; MOVEMENT OF PLANTS AND PLANT...

  11. 7 CFR 302.2 - Movement of plants and plant products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 5 2014-01-01 2014-01-01 false Movement of plants and plant products. 302.2 Section 302.2 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE DISTRICT OF COLUMBIA; MOVEMENT OF PLANTS AND PLANT...

  12. An integrated prediction and optimization model of biogas production system at a wastewater treatment facility.

    PubMed

    Akbaş, Halil; Bilgen, Bilge; Turhan, Aykut Melih

    2015-11-01

    This study proposes an integrated prediction and optimization model by using multi-layer perceptron neural network and particle swarm optimization techniques. Three different objective functions are formulated. The first one is the maximization of methane percentage with single output. The second one is the maximization of biogas production with single output. The last one is the maximization of biogas quality and biogas production with two outputs. Methane percentage, carbon dioxide percentage, and other contents' percentage are used as the biogas quality criteria. Based on the formulated models and data from a wastewater treatment facility, optimal values of input variables and their corresponding maximum output values are found out for each model. It is expected that the application of the integrated prediction and optimization models increases the biogas production and biogas quality, and contributes to the quantity of electricity production at the wastewater treatment facility. PMID:26295443

  13. Potential biodiesel and biogas production from corncob by anaerobic fermentation and black soldier fly.

    PubMed

    Li, Wu; Li, Qing; Zheng, Longyu; Wang, Yuanyuan; Zhang, Jibin; Yu, Ziniu; Zhang, Yanlin

    2015-10-01

    Bioenergy has become attractive as alternatives of gradually exhausted fossil fuel. Obtaining high grade bioenergy from lignocellulose is attractive that can gradually meet the demand. This study reported biogas and biodiesel were produced from corncob by a two-step bioprocess, biogas was produced from corncob by anaerobic fermentation, then biogas residue was converted by black soldier fly larvae, and then biodiesel was produced from larvae grease. 86.70 L biogas was obtained from 400 g corncob with the accumulation of biogas yield of 220.71 mL/g VS(added) by anaerobic digestion. Besides, 3.17 g of biodiesel was produced from grease after inoculating black soldier fly larvae into 400 g biogas residue. Meanwhile, the results showed that the addition of black soldier fly larvae could be effective for the degradation of lignocellulose and the accumulation of grease. PMID:26210140

  14. Monitoring of sulfur dioxide emission resulting from biogas utilization on commercial pig farms in Taiwan.

    PubMed

    Su, Jung-Jeng; Chen, Yen-Jung

    2015-01-01

    The objective of this work tends to promote methane content in biogas and evaluate sulfur dioxide emission from direct biogas combustion without desulfurization. Analytical results of biogas combustion showed that combustion of un-desulfurized biogas exhausted more than 92% of SO₂ (P < 0.01). In the meantime, more than 90% of hydrogen sulfide was removed during the combustion process using un-desulfurized biogas (P < 0.01). Those disappeared hydrogen sulfide may deposit on the surfaces of power generator's engines or burner heads of boilers. Some of them (4.6-9.1% of H₂S) were converted to SO₂ in exhaust gas. Considering the impacts to human health and living environment, it is better to desulfurize biogas before any applications. PMID:25404540

  15. Hydrogen sulfide removal from livestock biogas by a farm-scale bio-filter desulfurization system.

    PubMed

    Su, J-J; Chang, Y-C; Chen, Y-J; Chang, K-C; Lee, S-Y

    2013-01-01

    A farm-scale biogas desulfurization system was designed and tested for H2S removal efficiency from livestock biogas. This work assesses the H2S removal efficiency of a novel farm-scale biogas bio-desulfurization system (BBS) operated for 350 days on a 1,000-head pig farm. Experimental data demonstrated that suitable humidity and temperature can help sulfur-oxidizing bacteria to form active bio-films on the bio-carriers. The daily average removal rate increased to 879.16 from 337.75 g-H2S/d with an average inlet H2S concentration of 4,691 ± 1,532 mg/m(3) in biogas. Thus, the overall (0-350 days) average H2S removal efficiency exceeded 93%. The proposed BBS overcomes limitations of H2S in biogas when utilizing pig farm biogas for power generation and other applications. PMID:23508153

  16. Ultrasound pretreatment for enhanced biogas production from olive mill wastewater.

    PubMed

    Oz, Nilgun Ayman; Uzun, Alev Cagla

    2015-01-01

    This study investigates applicability of low frequency ultrasound technology to olive mill wastewaters (OMWs) as a pretreatment step prior to anaerobic batch reactors to improve biogas production and methane yield. OMWs originating from three phase processes are characterized with high organic content and complex nature. The treatment of the wastewater is problematic and alternative treatment options should be investigated. In the first part of the study, OMW samples were subjected to ultrasound at a frequency of 20kHz with applied powers varying between 50 and 100W under temperature controlled conditions for different time periods in order to determine the most effective sonication conditions. The level of organic matter solubilization at ultrasound experiments was assessed by calculating the ratio of soluble chemical oxygen demand/total chemical oxygen demand (SCOD/TCOD). The results revealed that the optimum ultrasonic condition for diluted OMW is 20kHz, 0.4W/mL for 10min. The application of ultrasound to OMW increased SCOD/TCOD ratio from 0.59 to 0.79. Statistical analysis (Friedman's tests) show that ultrasound was significantly effective on diluted OMW (p<0.05) in terms of SCOD parameter, but not for raw OMW (p>0.05). For raw OMW, this increase has been found to be limited due to high concentration of suspended solids (SS). In the second part of the study, biogas and methane production rates of anaerobic batch reactor fed with the ultrasound pretreated OMW samples were compared with the results of control reactor fed with untreated OMW in order to determine the effect of sonication. A nonparametric statistical procedure, Mann-Whitney U test, was used to compare biogas and methane production from anaerobic batch reactors for control and ultrasound pretreated samples. Results showed that application of low frequency ultrasound to OMW significantly improved both biogas and methane production in anaerobic batch reactor fed with the wastewater (p<0.05). Anaerobic

  17. Antimicrobial Resistance in Agriculture

    PubMed Central

    Thanner, Sophie; Drissner, David

    2016-01-01

    ABSTRACT In this article, the current knowledge and knowledge gaps in the emergence and spread of antimicrobial resistance (AMR) in livestock and plants and importance in terms of animal and human health are discussed. Some recommendations are provided for generation of the data required in order to develop risk assessments for AMR within agriculture and for risks through the food chain to animals and humans. PMID:27094336

  18. Methylotrophic bacteria in sustainable agriculture.

    PubMed

    Kumar, Manish; Tomar, Rajesh Singh; Lade, Harshad; Paul, Diby

    2016-07-01

    Excessive use of chemical fertilizers to increase production from available land has resulted in deterioration of soil quality. To prevent further soil deterioration, the use of methylotrophic bacteria that have the ability to colonize different habitats, including soil, sediment, water, and both epiphytes and endophytes as host plants, has been suggested for sustainable agriculture. Methylotrophic bacteria are known to play a significant role in the biogeochemical cycle in soil ecosystems, ultimately fortifying plants and sustaining agriculture. Methylotrophs also improve air quality by using volatile organic compounds such as dichloromethane, formaldehyde, methanol, and formic acid. Additionally, methylotrophs are involved in phosphorous, nitrogen, and carbon cycling and can help reduce global warming. In this review, different aspects of the interaction between methylotrophs and host plants are discussed, including the role of methylotrophs in phosphorus acquisition, nitrogen fixation, phytohormone production, iron chelation, and plant growth promotion, and co-inoculation of these bacteria as biofertilizers for viable agriculture practices. PMID:27263015

  19. Life Cycle Assessment of Biogas Production in Small-scale Household Digesters in Vietnam

    PubMed Central

    Vu, T. K. V.; Vu, D. Q.; Jensen, L. S.; Sommer, S. G.; Bruun, S.

    2015-01-01

    Small-scale household digesters have been promoted across Asia as a sustainable way of handling manure. The major advantages are that they produce biogas and reduce odor. However their disadvantages include the low recycling of nutrients, because digestate is dilute and therefore difficult to transport, and the loss of biogas as a result of cracks and the intentional release of excess biogas. In this study, life cycle assessment (LCA) methodology was used to assess the environmental impacts associated with biogas digesters in Vietnam. Handling 1,000 kg of liquid manure and 100 kg of solid manure in a system with a biogas digester reduced the impact potential from 4.4 kg carbon dioxide (CO2) equivalents to 3.2 kg CO2 equivalents compared with traditional manure management. However, this advantage could easily be compromised if digester construction is considered in the LCA or in situations where there is an excess of biogas which is intentionally released. A sensitivity analysis showed that biogas digesters could be a means of reducing global warming if methane emissions can be kept low. In terms of eutrophication, farms with biogas digesters had 3 to 4 times greater impacts. In order to make biogas digesters sustainable, methods for recycling digestates are urgently required. PMID:25715690

  20. Life Cycle Assessment of Biogas Production in Small-scale Household Digesters in Vietnam.

    PubMed

    Vu, T K V; Vu, D Q; Jensen, L S; Sommer, S G; Bruun, S

    2015-05-01

    Small-scale household digesters have been promoted across Asia as a sustainable way of handling manure. The major advantages are that they produce biogas and reduce odor. However their disadvantages include the low recycling of nutrients, because digestate is dilute and therefore difficult to transport, and the loss of biogas as a result of cracks and the intentional release of excess biogas. In this study, life cycle assessment (LCA) methodology was used to assess the environmental impacts associated with biogas digesters in Vietnam. Handling 1,000 kg of liquid manure and 100 kg of solid manure in a system with a biogas digester reduced the impact potential from 4.4 kg carbon dioxide (CO2) equivalents to 3.2 kg CO2 equivalents compared with traditional manure management. However, this advantage could easily be compromised if digester construction is considered in the LCA or in situations where there is an excess of biogas which is intentionally released. A sensitivity analysis showed that biogas digesters could be a means of reducing global warming if methane emissions can be kept low. In terms of eutrophication, farms with biogas digesters had 3 to 4 times greater impacts. In order to make biogas digesters sustainable, methods for recycling digestates are urgently required. PMID:25715690