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Sample records for agriculturally important insect

  1. AN INSECT PEST FOR AGRICULTURAL, URBAN, AND WILDLIFE AREAS: THE RED IMPORTED FIRE ANT

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Red Imported Fire Ant (RIFA), Solenopsis invicta (Buren), is an insect pest of particular importance in California due to its potential impact on public health, agriculture, and wildlife. In 1997, RIFAs hitchhiked to the Central Valley on honeybee hives brought in from Texas for pollination of a...

  2. Important Insect Pests of Fruit - Important Insect Pests of Nuts - Field Crop Insect Pests - Insect Pests of Vegetable Crops.

    ERIC Educational Resources Information Center

    Gesell, Stanley G.; And Others

    This document consists of four agriculture extension service publications from Pennsylvania State University. The titles are: (1) Important Insect Pests of Fruit; (2) Important Insect Pests of Nuts; (3) Field Crop Insect Pests; and (4) Insect Pests of Vegetable Crops. The first publication gives the hosts, injury, and description of 22 insect…

  3. Monitoring the agricultural landscape for insect resistance

    NASA Astrophysics Data System (ADS)

    Casas, Joseph; Glaser, J. A.; Copenhaver, Ken

    Farmers in 25 countries on six continents are using plant biotechnology to solve difficult crop production challenges and conserve the environment. In fact, 13.3 million farmers, which include 90 percent of the farming in developing countries, choose to plant biotech crops. Over the past decade, farmers increased area planted in genetically modified (GM) crops by more than 10 percent each year, thus increasing their farm income by more than 44 billion US dollars (1996-2007), and achieved economic, environmental and social benefits in crops such as soybeans, canola, corn and cotton. To date, total acres of biotech crops harvested exceed more than 2 billion with a proven 13-year history of safe use. Over the next decade, expanded adoption combined with current research on 57 crops in 63 countries will broaden the advantages of genetically modified foods for growers, consumers and the environment. Genetically modified (GM) crops with the ability to produce toxins lethal to specific insect pests are covering a larger percentage of the agricultural landscape every year. The United States department of Agriculture (USDA) estimated that 63 percent of corn and 65 percent of cotton contained these specific genetic traits in 2009. The toxins could protect billions of dollars of loss from insect damage for crops valued at greater than 165 billion US dollars in 2008. The stable and efficient production of these crops has taken on even more importance in recent years with their use, not only as a food source, but now also a source of fuel. It is in the best interest of the United States Environmental Protection Agency (USEPA) to ensure the continued efficacy of toxin producing GM crops as their use reduces pesticides harmful to humans and animals. However, population genetics models have indicated the risk of insect pests developing resistance to these toxins if a high percentage of acreage is grown in these crops. The USEPA is developing methods to monitor the agricultural

  4. Agricultural applications of insect ecological genomics.

    PubMed

    Poelchau, Monica F; Coates, Brad S; Childers, Christopher P; Peréz de León, Adalberto A; Evans, Jay D; Hackett, Kevin; Shoemaker, DeWayne

    2016-02-01

    Agricultural entomology is poised to benefit from the application of ecological genomics, particularly the fields of biofuels generation and pest control. Metagenomic methods can characterize microbial communities of termites, wood-boring beetles and livestock pests, and transcriptomic approaches reveal molecular bases behind wood-digesting capabilities of these insects, leading to potential mechanisms for biofuel generation. Genome sequences are being exploited to develop new pest control methods, identify candidate antigens to vaccinate livestock, and discover RNAi target sequences and potential non-target effects in other insects. Gene content analyses of pest genome sequences and their endosymbionts suggest metabolic interdependencies between organisms, exposing potential gene targets for insect control. Finally, genome-wide association studies and genotyping by high-throughput sequencing promise to improve management of pesticide resistance. PMID:27436554

  5. Plant or fungal-produced conophthorin as an important component of host plant volatile-based attractants for agricultural lepidopteran insect pests

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Conophthorin (7-methyl-1,6-dioxaspiro[4.5]decane) is a semiochemical common to numerous coleopteran and hymenopteran insects, and possess varying semiochemical behavior among these species. Conophthorin has recently gained attention as a semiochemical for navel orangeworm, Amyelois transitella (Lepi...

  6. Economic importance of bats in agriculture

    USGS Publications Warehouse

    Boyles, Justin G.; Cryan, Paul M.; McCracken, Gary F.; Kunz, Thomas H.

    2011-01-01

    White-nose syndrome (WNS) and the increased development of wind-power facilities are threatening populations of insectivorous bats in North America. Bats are voracious predators of nocturnal insects, including many crop and forest pests. We present here analyses suggesting that loss of bats in North America could lead to agricultural losses estimated at more than $3.7 billion/year. Urgent efforts are needed to educate the public and policy-makers about the ecological and economic importance of insectivorous bats and to provide practical conservation solutions.

  7. Invasion and Management of Agricultural Alien Insects in China.

    PubMed

    Wan, Fang-Hao; Yang, Nian-Wan

    2016-01-01

    China is the world's fourth-largest country in terms of landmass. Its highly diverse biogeography presents opportunities for many invasive alien insects. However, physical and climate barriers sometimes prevent locally occurring species from spreading. China has 560 confirmed invasive alien species; 125 are insect pests, and 92 of these damage the agricultural ecosystem. The estimated annual economic loss due to alien invasive species is more than $18.9 billion. The most harmful invasive insects exhibit some common characteristics, such as high reproduction, competitive dominance, and high tolerance, and benefit from mutualist facilitation interactions. Regional cropping system structure adjustments have resulted in mono-agricultural ecosystems in cotton and other staple crops, providing opportunities for monophagous insect pests. Furthermore, human dietary shifts to fruits and vegetables and smallholder-based farming systems result in highly diverse agricultural ecosystems, which provide resource opportunities for polyphagous insects. Multiple cropping and widespread use of greenhouses provide continuous food and winter habitats for insect pests, greatly extending their geographic range. The current management system consists of early-warning, monitoring, eradication, and spread blocking technologies. This review provides valuable new synthetic information on integrated management practices based mainly on biological control for a number of invasive species. We encourage farmers and extension workers to be more involved in training and further research for novel protection methods that takes into consideration end users' needs. PMID:26527302

  8. Optical characterization of agricultural pest insects: a methodological study in the spectral and time domains

    NASA Astrophysics Data System (ADS)

    Li, Y. Y.; Zhang, H.; Duan, Z.; Lian, M.; Zhao, G. Y.; Sun, X. H.; Hu, J. D.; Gao, L. N.; Feng, H. Q.; Svanberg, S.

    2016-08-01

    Identification of agricultural pest insects is an important aspect in insect research and agricultural monitoring. We have performed a methodological study of how spectroscopic techniques and wing-beat frequency analysis might provide relevant information. An optical system based on the combination of close-range remote sensing and reflectance spectroscopy was developed to study the optical characteristics of different flying insects, collected in Southern China. The results demonstrate that the combination of wing-beat frequency assessment and reflectance spectral analysis has the potential to successfully differentiate between insect species. Further, studies of spectroscopic characteristics of fixed specimen of insects, also from Central China, showed the possibility of refined agricultural pest identification. Here, in addition to reflectance recordings also laser-induced fluorescence spectra were investigated for all the species of insects under study and found to provide complementary information to optically distinguish insects. In order to prove the practicality of the techniques explored, clearly fieldwork aiming at elucidating the variability of parameters, even within species, must be performed.

  9. Managing social insects of urban importance.

    PubMed

    Rust, Michael K; Su, Nan-Yao

    2012-01-01

    Social insects have a tremendous economic and social impact on urban communities. The rapid urbanization of the world has dramatically increased the incidence of urban pests. Human commerce has resulted in the spread of urban invasive species worldwide such that various species are now common to many major urban centers. We aim to highlight those social behaviors that can be exploited to control these pests with the minimal use of pesticides. Their cryptic behavior often prohibits the direct treatment of colonies. However, foraging and recruitment are essential aspects of their social behavior and expose workers to traps, baits, and pesticide applications. The advent of new chemistries has revolutionized the pest management strategies used to control them. In recent years, there has been an increased environmental awareness, especially in the urban community. Advances in molecular and microbial agents promise additional tools in developing integrated pest management programs against social insects. PMID:21942844

  10. Convergent bacterial microbiotas in the fungal agricultural systems of insects

    DOE PAGESBeta

    Aylward, Frank O.; Suen, Garret; Biedermann, Peter H. W.; Adams, Aaron S.; Scott, Jarrod J.; Malfatti, Stephanie A.; Glavina del Rio, Tijana; Tringe, Susannah G.; Poulsen, Michael; Raffa, Kenneth F.; et al

    2014-11-18

    The ability to cultivate food is an innovation that has produced some of the most successful ecological strategies on the planet. Although most well recognized in humans, where agriculture represents a defining feature of civilization, species of ants, beetles, and termites have also independently evolved symbioses with fungi that they cultivate for food. Despite occurring across divergent insect and fungal lineages, the fungivorous niches of these insects are remarkably similar, indicating convergent evolution toward this successful ecological strategy. Here, we characterize the microbiota of ants, beetles, and termites engaged in nutritional symbioses with fungi to define the bacterial groups associatedmore » with these prominent herbivores and forest pests. Using culture-independent techniques and the in silico reconstruction of 37 composite genomes of dominant community members, we demonstrate that different insect-fungal symbioses that collectively shape ecosystems worldwide have highly similar bacterial microbiotas comprised primarily of the genera Enterobacter, Rahnella, and Pseudomonas. Although these symbioses span three orders of insects and two phyla of fungi, we show that they are associated with bacteria sharing high whole-genome nucleotide identity. Due to the fine-scale correspondence of the bacterial microbiotas of insects engaged in fungal symbioses, our findings indicate that this represents an example of convergence of entire host-microbe complexes.« less

  11. Convergent bacterial microbiotas in the fungal agricultural systems of insects

    SciTech Connect

    Aylward, Frank O.; Suen, Garret; Biedermann, Peter H. W.; Adams, Aaron S.; Scott, Jarrod J.; Malfatti, Stephanie A.; Glavina del Rio, Tijana; Tringe, Susannah G.; Poulsen, Michael; Raffa, Kenneth F.; Klepzig, Kier D.; Currie, Cameron R.

    2014-11-18

    The ability to cultivate food is an innovation that has produced some of the most successful ecological strategies on the planet. Although most well recognized in humans, where agriculture represents a defining feature of civilization, species of ants, beetles, and termites have also independently evolved symbioses with fungi that they cultivate for food. Despite occurring across divergent insect and fungal lineages, the fungivorous niches of these insects are remarkably similar, indicating convergent evolution toward this successful ecological strategy. Here, we characterize the microbiota of ants, beetles, and termites engaged in nutritional symbioses with fungi to define the bacterial groups associated with these prominent herbivores and forest pests. Using culture-independent techniques and the in silico reconstruction of 37 composite genomes of dominant community members, we demonstrate that different insect-fungal symbioses that collectively shape ecosystems worldwide have highly similar bacterial microbiotas comprised primarily of the genera Enterobacter, Rahnella, and Pseudomonas. Although these symbioses span three orders of insects and two phyla of fungi, we show that they are associated with bacteria sharing high whole-genome nucleotide identity. Due to the fine-scale correspondence of the bacterial microbiotas of insects engaged in fungal symbioses, our findings indicate that this represents an example of convergence of entire host-microbe complexes.

  12. Convergent Bacterial Microbiotas in the Fungal Agricultural Systems of Insects

    PubMed Central

    Suen, Garret; Biedermann, Peter H. W.; Adams, Aaron S.; Scott, Jarrod J.; Malfatti, Stephanie A.; Glavina del Rio, Tijana; Tringe, Susannah G.; Poulsen, Michael; Raffa, Kenneth F.; Klepzig, Kier D.

    2014-01-01

    ABSTRACT The ability to cultivate food is an innovation that has produced some of the most successful ecological strategies on the planet. Although most well recognized in humans, where agriculture represents a defining feature of civilization, species of ants, beetles, and termites have also independently evolved symbioses with fungi that they cultivate for food. Despite occurring across divergent insect and fungal lineages, the fungivorous niches of these insects are remarkably similar, indicating convergent evolution toward this successful ecological strategy. Here, we characterize the microbiota of ants, beetles, and termites engaged in nutritional symbioses with fungi to define the bacterial groups associated with these prominent herbivores and forest pests. Using culture-independent techniques and the in silico reconstruction of 37 composite genomes of dominant community members, we demonstrate that different insect-fungal symbioses that collectively shape ecosystems worldwide have highly similar bacterial microbiotas comprised primarily of the genera Enterobacter, Rahnella, and Pseudomonas. Although these symbioses span three orders of insects and two phyla of fungi, we show that they are associated with bacteria sharing high whole-genome nucleotide identity. Due to the fine-scale correspondence of the bacterial microbiotas of insects engaged in fungal symbioses, our findings indicate that this represents an example of convergence of entire host-microbe complexes. PMID:25406380

  13. Resistance to sap-sucking insects in modern-day agriculture

    PubMed Central

    VanDoorn, Arjen; de Vos, Martin

    2013-01-01

    Plants and herbivores have co-evolved in their natural habitats for about 350 million years, but since the domestication of crops, plant resistance against insects has taken a different turn. With the onset of monoculture-driven modern agriculture, selective pressure on insects to overcome resistances has dramatically increased. Therefore plant breeders have resorted to high-tech tools to continuously create new insect-resistant crops. Efforts in the past 30 years have resulted in elucidation of mechanisms of many effective plant defenses against insect herbivores. Here, we critically appraise these efforts and – with a focus on sap-sucking insects – discuss how these findings have contributed to herbivore-resistant crops. Moreover, in this review we try to assess where future challenges and opportunities lay ahead. Of particular importance will be a mandatory reduction in systemic pesticide usage and thus a greater reliance on alternative methods, such as improved plant genetics for plant resistance to insect herbivores. PMID:23818892

  14. RNAi in agriculturally-important arthropods

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Our aim in this chapter is to provide an overview of the profound knowledge accumulated in recent years from invertebrate RNAi studies, but with a focus on agriculturally important arthropods. We start with a brief discussion of the RNAi mechanism to introduce readers to key concepts that underlie t...

  15. Genetic control of insects of public health importance

    PubMed Central

    Knipling, E. F.; Laven, H.; Craig, G. B.; Pal, R.; Kitzmiller, J. B.; Smith, C. N.; Brown, A. W. A.

    1968-01-01

    During recent years many advances have been made in the development of insect control by genetic manipulation. These methods include the sterile-male technique, now well known, which depends on ionizing radiation or chemosterilization. The recent field experiment carried out by WHO in Rangoon, Burma, on Culex fatigans has demonstrated that naturally occurring cytogenetic mechanisms such as cytoplasmic incompatibility can be used successfully without the use of radiations or chemosterilants. The paper not only describes the experiment on Culex fatigans but also discusses basic concepts and theoretical considerations involved in genetic control of insects of public health importance. The possibility of using genetic mechanisms for the control of other vector species is also discussed. There are a number of problems which require study before genetic control can be used on an operational scale. These problems and suggestions for future research in this field are also outlined. PMID:5302334

  16. A survey of insect assemblages responding to volatiles from a ubiquitous fungus in an agricultural landscape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We report here a first survey of insect orientation to fungal cultures and and fungal volatiles from a community ecology perspective. We tested whether volatiles from a ubiquitous yeast-like fungus (Aureobasidium pullulans) are broadly attractive to insects in an agricultural landscape. We evaluated...

  17. BATS AND BT INSECT RESISTANCE ON AGRICULTURAL LANDSCAPES

    EPA Science Inventory

    A landscape model that utilizes land cover classification data, insect life history, insect movement, and bat foraging pressure is developed that addresses the implementation of genetically modified crops in the Winter Garden region of Texas. The principal strategy for delaying r...

  18. A survey of insect assemblages responding to volatiles from a ubiquitous fungus in an agricultural landscape.

    PubMed

    Davis, Thomas Seth; Landolt, Peter J

    2013-07-01

    We report here a first survey of insect orientation to fungal cultures and fungal volatiles from a community ecology perspective. We tested whether volatiles from a ubiquitous yeast-like fungus (Aureobasidium pullulans) are broadly attractive to insects in an agricultural landscape. We evaluated insect attraction to fungal cultures and synthetic compounds identified in fungal headspace (2-methyl-1-butanol, 3-methyl-1-butanol, 2-phenylethanol) in a spearmint (Mentha spicata L.) plantation. Three findings emerged: (1) 1,315 insects representing seven orders and 39 species oriented to traps, but 65 % of trapped insects were Dipterans, of which 80 % were hoverflies (Diptera: Syrphidae); (2) traps baited with A. pullulans caught 481 % more insects than unbaited control traps on average, and contained more diverse (Shannon's H index) and species rich assemblages than control traps, traps baited with Penicillium expansum, or uninoculated media; and (3) insects oriented in greatest abundance to a 1:1:1 blend of A. pullulans volatiles, but mean diversity scores were highest for traps baited with only 2-phenylethanol or 2-methyl-1-butanol. Our results show that individual components of fungal headspace are not equivalent in terms of the abundance and diversity of insects that orient to them. The low abundance of insects captured with P. expansum suggests that insect assemblages do not haphazardly orient to fungal volatiles. We conclude that volatiles from a common fungal species (A. pullulans) are attractive to a variety of insect taxa in an agricultural system, and that insect orientation to fungal volatiles may be a common ecological phenomenon. PMID:23564294

  19. Insect Barcode Information System

    PubMed Central

    Pratheepa, Maria; Jalali, Sushil Kumar; Arokiaraj, Robinson Silvester; Venkatesan, Thiruvengadam; Nagesh, Mandadi; Panda, Madhusmita; Pattar, Sharath

    2014-01-01

    Insect Barcode Information System called as Insect Barcode Informática (IBIn) is an online database resource developed by the National Bureau of Agriculturally Important Insects, Bangalore. This database provides acquisition, storage, analysis and publication of DNA barcode records of agriculturally important insects, for researchers specifically in India and other countries. It bridges a gap in bioinformatics by integrating molecular, morphological and distribution details of agriculturally important insects. IBIn was developed using PHP/My SQL by using relational database management concept. This database is based on the client– server architecture, where many clients can access data simultaneously. IBIn is freely available on-line and is user-friendly. IBIn allows the registered users to input new information, search and view information related to DNA barcode of agriculturally important insects.This paper provides a current status of insect barcode in India and brief introduction about the database IBIn. Availability http://www.nabg-nbaii.res.in/barcode PMID:24616562

  20. Dispersal strategies of phytophagous insects at a local scale: adaptive potential of aphids in an agricultural environment

    PubMed Central

    Lombaert, Eric; Boll, Roger; Lapchin, Laurent

    2006-01-01

    Background The spread of agriculture greatly modified the selective pressures exerted by plants on phytophagous insects, by providing these insects with a high-level resource, structured in time and space. The life history, behavioural and physiological traits of some insect species may have evolved in response to these changes, allowing them to crowd on crops and to become agricultural pests. Dispersal, which is one of these traits, is a key concept in evolutionary biology but has been over-simplified in most theoretical studies. We evaluated the impact of the local-scale dispersal strategy of phytophagous insects on their fitness, using an individual-based model to simulate population dynamics and dispersal between leaves and plants, by walking and flying, of the aphid Aphis gossypii, a major agricultural pest, in a melon field. We compared the optimal values for dispersal parameters in the model with the corresponding observed values in experimental trials. Results We show that the rates of walking and flying disperser production on leaves were the most important traits determining the fitness criteria, whereas dispersal distance and the clustering of flying dispersers on the target plant had no effect. We further show that the effect of dispersal parameters on aphid fitness depended strongly on plant characteristics. Conclusion Parameters defining the dispersal strategies of aphids at a local scale are key components of the fitness of these insects and may thus be essential in the adaptation to agricultural environments that are structured in space and time. Moreover, the fact that the effect of dispersal parameters on aphid fitness depends strongly on plant characteristics suggests that traits defining aphid dispersal strategies may be a cornerstone of host-plant specialization. PMID:17014710

  1. Role of nanotechnology in agriculture with special reference to management of insect pests.

    PubMed

    Rai, Mahendra; Ingle, Avinash

    2012-04-01

    Nanotechnology is a promising field of interdisciplinary research. It opens up a wide array of opportunities in various fields like medicine, pharmaceuticals, electronics and agriculture. The potential uses and benefits of nanotechnology are enormous. These include insect pests management through the formulations of nanomaterials-based pesticides and insecticides, enhancement of agricultural productivity using bio-conjugated nanoparticles (encapsulation) for slow release of nutrients and water, nanoparticle-mediated gene or DNA transfer in plants for the development of insect pest-resistant varieties and use of nanomaterials for preparation of different kind of biosensors, which would be useful in remote sensing devices required for precision farming. Traditional strategies like integrated pest management used in agriculture are insufficient, and application of chemical pesticides like DDT have adverse effects on animals and human beings apart from the decline in soil fertility. Therefore, nanotechnology would provide green and efficient alternatives for the management of insect pests in agriculture without harming the nature. This review is focused on traditional strategies used for the management of insect pests, limitations of use of chemical pesticides and potential of nanomaterials in insect pest management as modern approaches of nanotechnology. PMID:22388570

  2. Mycotoxin contamination of commercially important agricultural commodities

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fungal toxins, called aflatoxins and other mold toxins, are a serious problem in US agricultural commodities. Due to aflatoxins resilience to industrial processes contaminated crops (corn, cotton, peanuts, and tree nuts) cannot be used. The loss of these commodities results in serious economic impa...

  3. A proboscis extension response protocol for investigating behavioral plasticity in insects: application to basic, biomedical, and agricultural research.

    PubMed

    Smith, Brian H; Burden, Christina M

    2014-01-01

    Insects modify their responses to stimuli through experience of associating those stimuli with events important for survival (e.g., food, mates, threats). There are several behavioral mechanisms through which an insect learns salient associations and relates them to these events. It is important to understand this behavioral plasticity for programs aimed toward assisting insects that are beneficial for agriculture. This understanding can also be used for discovering solutions to biomedical and agricultural problems created by insects that act as disease vectors and pests. The Proboscis Extension Response (PER) conditioning protocol was developed for honey bees (Apis mellifera) over 50 years ago to study how they perceive and learn about floral odors, which signal the nectar and pollen resources a colony needs for survival. The PER procedure provides a robust and easy-to-employ framework for studying several different ecologically relevant mechanisms of behavioral plasticity. It is easily adaptable for use with several other insect species and other behavioral reflexes. These protocols can be readily employed in conjunction with various means for monitoring neural activity in the CNS via electrophysiology or bioimaging, or for manipulating targeted neuromodulatory pathways. It is a robust assay for rapidly detecting sub-lethal effects on behavior caused by environmental stressors, toxins or pesticides. We show how the PER protocol is straightforward to implement using two procedures. One is suitable as a laboratory exercise for students or for quick assays of the effect of an experimental treatment. The other provides more thorough control of variables, which is important for studies of behavioral conditioning. We show how several measures for the behavioral response ranging from binary yes/no to more continuous variable like latency and duration of proboscis extension can be used to test hypotheses. And, we discuss some pitfalls that researchers commonly encounter

  4. A Proboscis Extension Response Protocol for Investigating Behavioral Plasticity in Insects: Application to Basic, Biomedical, and Agricultural Research

    PubMed Central

    Smith, Brian H.; Burden, Christina M.

    2014-01-01

    Insects modify their responses to stimuli through experience of associating those stimuli with events important for survival (e.g., food, mates, threats). There are several behavioral mechanisms through which an insect learns salient associations and relates them to these events. It is important to understand this behavioral plasticity for programs aimed toward assisting insects that are beneficial for agriculture. This understanding can also be used for discovering solutions to biomedical and agricultural problems created by insects that act as disease vectors and pests. The Proboscis Extension Response (PER) conditioning protocol was developed for honey bees (Apis mellifera) over 50 years ago to study how they perceive and learn about floral odors, which signal the nectar and pollen resources a colony needs for survival. The PER procedure provides a robust and easy-to-employ framework for studying several different ecologically relevant mechanisms of behavioral plasticity. It is easily adaptable for use with several other insect species and other behavioral reflexes. These protocols can be readily employed in conjunction with various means for monitoring neural activity in the CNS via electrophysiology or bioimaging, or for manipulating targeted neuromodulatory pathways. It is a robust assay for rapidly detecting sub-lethal effects on behavior caused by environmental stressors, toxins or pesticides. We show how the PER protocol is straightforward to implement using two procedures. One is suitable as a laboratory exercise for students or for quick assays of the effect of an experimental treatment. The other provides more thorough control of variables, which is important for studies of behavioral conditioning. We show how several measures for the behavioral response ranging from binary yes/no to more continuous variable like latency and duration of proboscis extension can be used to test hypotheses. And, we discuss some pitfalls that researchers commonly encounter

  5. Beneficial Insects and Insect Pollinators on Milkweed in South Georgia

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insect pollinators are essential for the reproduction of more than two-thirds of the world’s crops, and beneficial insects play an important role in managing pest insects in agricultural farmscapes. These insects depend on nectar for their survival in these farmscapes. The flowers of tropical milkwe...

  6. Olfactory disruption: towards controlling important insect vectors of disease

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Chemical repellents are used to decrease contacts between insect disease vectors and their hosts, thus reducing the probability of disease transmission. The molecular mechanisms by which repellents have their effects are poorly understood and remain a controversial topic. Here we present recent re...

  7. Importance of energy balance in agriculture.

    NASA Astrophysics Data System (ADS)

    Meco, R.; Moreno, M. M.; Lacasta, C.; Tarquis, A. M.; Moreno, C.

    2012-04-01

    Since the beginning, man has tried to control nature and the environment, and the use of energy, mainly from non-renewable sources providing the necessary power for that. The consequences of this long fight against nature has reached a critical state of unprecedented worldwide environmental degradation, as evidenced by the increasing erosion of fertile lands, the deforestation processes, the pollution of water, air and land by agrochemicals, the loss of plant and animal species, the progressive deterioration of the ozone layer and signs of global warming. This is exacerbated by the increasing population growth, implying a steady increase in consumption, and consequently, in the use of energy. Unfortunately, all these claims are resulting in serious economic and environmental problems worldwide. Because the economic and environmental future of the countries is interrelated, it becomes necessary to adopt sustainable development models based on the use of renewable and clean energies, the search for alternative resources and the use of productive systems more efficient from an energy standpoint, always with a reduction of greenhouse gas emissions. In relation to the agricultural sector, the question we ask is: how long can we keep the current energy-intensive agricultural techniques in developed countries? To analyze this aspect, energy balance is a very helpful tool because can lead to more efficient, sustainable and environment-friendly production systems for each agro-climatic region. This requires the identification of all the inputs and the outputs involved and their conversion to energy values by means of corresponding energy coefficients or equivalents (International Federation of Institutes for Advanced Studies). Energy inputs (EI) can be divided in direct (energy directly used in farms as fuel, machines, fertilizers, seeds, herbicides, human labor, etc.) and indirect (energy not consumed in the farm but in the elaboration, manufacturing or manipulation of

  8. The Study of Importance of the Balance Space Food with Insects

    NASA Astrophysics Data System (ADS)

    Katayama, Naomi; Yoshimura, Tuyoshi; Baba, Keiichi; Space Agriculture Task Force, J.

    Providing foods to space crew is the important requirements to support long term manned space exploration. We designed joyful and healthy recipe with materials (plants, insects, fish and et.cet.), which can be produced by the bio-regenerative agricultural system operated at limited resources available in spaceship or on Moon and Mars. The fundamental composition of our recipe is unpolished rice, barley, soybean, sweat potato and green-yellow vegetables. Supplement food materials to fulfill the nutritional requirements we chose are loach, silkworm pupa, termite, snail, mud snail, bee, locust, cassava, quinoa and et. cet. And the space foods were made by using IH cooking heater and vacuum cooking machine. Nutritional analysis on the basic vegetable menu consisting of rice, barley, soybean, sweet potato cassava, quinoa and green reveals a shortage of vitamins D and B12, cholesterol and sodium salt. Since vitamin D deficiency results in demineralization of bone. Vitamin B12 is essential to prevent pernicious anemia.Fish contains both vitamins D and B12. The pupa of the silkworm becomes the impor-tant nourishment source as protein and lipid. The silk thread uses it as clothing and cosmetics and medical supplies. However, we can use the silk thread as food as protein after finish to use. A law of nature shakes high quality oils and fats included in termite for cooking. I use the bee as food after having used it for the pollination of the plant. Of course the honey becomes the important food, too. The snail and mud snail become the food as protein. We decided to use the menu consisting of the basic vegetarian menu plus insect and loach for further conceptual design of space agriculture. We succeeded to develop joyful and nutritious space recipe at the end. Because of less need of agricultural resources at choosing ecological members from the lower ladder of the food chain, our space recipe could be a proposal to solve the food problem on Earth.In addition, as for these

  9. The Importance of Agriculture Science Course Sequencing in High Schools: A View from Collegiate Agriculture Students

    ERIC Educational Resources Information Center

    Wheelus, Robin P.

    2009-01-01

    The objective of this study was to investigate the importance of Agriculture Science course sequencing in high schools, as a preparatory factor for students enrolled in collegiate agriculture classes. With the variety of courses listed in the Texas Essential Knowledge and Skills (TEKS) for Agriculture Science, it has been possible for counselors,…

  10. Geospatial Modeling and Disease Insect Vector Management at the USDA-ARS Center for Medical, Agricultural, and Veterinary Entomology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Geospatial modeling at the Center for Medical, Agricultural and Veterinary Entomology (CMAVE) is used assist in the surveillance of insect vectors and in the management of insect transmitted diseases. The most recent Geospatial Modeling/Technology Transfer success involves the prediction of Rift Val...

  11. Linking agricultural practices, mycorrhizal fungi, and traits mediating plant-insect interactions.

    PubMed

    Barber, Nicholas A; Kiers, E Toby; Theis, Nina; Hazzard, Ruth V; Adler, Lynn S

    2013-10-01

    Agricultural management has profound effects on soil communities. Activities such as fertilizer inputs can modify the composition of arbuscular mycorrhizal fungi (AMF) communities, which form important symbioses with the roots of most crop plants. Intensive conventional agricultural management may select for less mutualistic AMF with reduced benefits to host plants compared to organic management, but these differences are poorly understood. AMF are generally evaluated based on their direct growth effects on plants. However, mycorrhizal colonization also may alter plant traits such as tissue nutrients, defensive chemistry, or floral traits, which mediate important plant-insect interactions like herbivory and pollination. To determine the effect of AMF from different farming practices on plant performance and traits that putatively mediate species interactions, we performed a greenhouse study by inoculating Cucumis sativus (cucumber, Cucurbitaceae) with AMF from conventional farms, organic farms, and a commercial AMF inoculum. We measured growth and a suite of plant traits hypothesized to be important predictors of herbivore resistance and pollinator attraction. Several leaf and root traits and flower production were significantly affected by AMF inoculum. Both conventional and organic AMF reduced leaf P content but increased Na content compared to control and commercial AMF. Leaf defenses were unaffected by AMF treatments, but conventional AMF increased root cucurbitacin C, the primary defensive chemical of C. sativus, compared to organic AMF. These effects may have important consequences for herbivore preference and population dynamics. AMF from both organic and conventional farms decreased flower production relative to commercial and control treatments, which may reduce pollinator attraction and plant reproduction. AMF from both farm types also reduced seed germination, but effects on plant growth were limited. Our results suggest that studies only considering AMF

  12. Aquatic Insect Emergence in Post-Harvest Flooded Agricultural Fields in the Southern San Joaquin Valley, California

    NASA Astrophysics Data System (ADS)

    Moss, R. C.; Blumenshine, S.; Fleskes, J.

    2005-05-01

    California's Southern San Joaquin Valley is one of the most important waterbird areas in North America, but has suffered a disproportionate loss of wetlands when compared to other California regions. This project analyzes the habitat value of post-harvest flooded cropland by measuring the emergence of aquatic insects across multiple crop types. Aquatic insect emergence was sampled from post-harvest flooded fields of four crop types (alfalfa, corn, tomato, wheat), August-October, 2003-2004. Emergence was measured using traps deployed with a stratified random distribution to sample between and within field variation. Emergence rate and emergent biomass was significantly higher in flooded tomato fields. Results from corn fields indicate that flooding depth was correlated (r=0.095) with both diel temperature fluctuation and emergence rate. Chironomus dilutus larvae were grown in environmental chambers, under two thermal treatments with the same mean but different amplitudes (high: 15°-32°C, low: 20°-26°C) to investigate thermal fluctuation effects on survival and biomass. Larval survival (4x) and biomass (2x) were significantly greater in the low versus high temperature fluctuation treatment. This research has the potential to affect agricultural management throughout the 12,600 km2 region, increase aquatic insect production and aid in the recovery of declining bird populations.

  13. Sugarcane insect update

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insect are an important group of pests affecting sugarcane production. Agricultural consultants play an important role is assisting sugarcane farmers to choose the most appropriated means of managing damaging infestations of insects in their crop. In this presentation, information will be presented ...

  14. Insect pests of sweetpotato in Uganda: farmers' perceptions of their importance and control practices.

    PubMed

    Okonya, Joshua Sikhu; Mwanga, Robert Om; Syndikus, Katja; Kroschel, Jürgen

    2014-01-01

    Insect pests are among the most important constraints limiting sweetpotato (Ipomoea batatas) production in Africa. However, there is inadequate information about farmers' knowledge, perceptions and practices in the management of key insect pests. This has hindered development of effective pest management approaches for smallholder farmers. A standard questionnaire was used to interview individual sweetpotato farmers (n = 192) about their perception and management practices regarding insect pests in six major sweetpotato producing districts of Uganda. The majority (93%) of farmers perceived insect pests to be a very serious problem. With the exception of Masindi and Wakiso districts where the sweetpotato butterfly (Acraea acerata) was the number one constraint, sweetpotato weevils (Cylas puncticollis and C. brunneus) were ranked as the most important insect pests. Insecticide use in sweetpotato fields was very low being highest (28-38% of households) in districts where A. acerata infestation is the biggest problem. On average, 65% and 87% of the farmers took no action to control A. acerata and Cylas spp., respectively. Farmers were more conversant with the presence of and damage by A. acerata than of Cylas spp. as they thought that Cylas spp. root damage was brought about by a prolonged dry season. Different levels of field resistance (ability of a variety to tolerate damage) of sweetpotato landraces to A. acerata (eight landraces) and Cylas spp. (six landraces) were reported by farmers in all the six districts. This perceived level of resistance to insect damage by landraces needs to be investigated. To improve farmers' capabilities for sweetpotato insect pest management, it is crucial to train them in the basic knowledge of insect pest biology and control. PMID:25279278

  15. The Importance of Landscape Elements for Bat Activity and Species Richness in Agricultural Areas.

    PubMed

    Heim, Olga; Treitler, Julia T; Tschapka, Marco; Knörnschild, Mirjam; Jung, Kirsten

    2015-01-01

    Landscape heterogeneity is regarded as a key factor for maintaining biodiversity and ecosystem function in production landscapes. We investigated whether grassland sites at close vicinity to forested areas are more frequently used by bats. Considering that bats are important consumers of herbivorous insects, including agricultural pest, this is important for sustainable land management. Bat activity and species richness were assessed using repeated monitoring from May to September in 2010 with acoustic monitoring surveys on 50 grassland sites in the Biosphere Reserve Schorfheide-Chorin (North-East Germany). Using spatial analysis (GIS), we measured the closest distance of each grassland site to potentially connecting landscape elements (e.g., trees, linear vegetation, groves, running and standing water). In addition, we assessed the distance to and the percent land cover of forest remnants and urban areas in a 200 m buffer around the recording sites to address differences in the local landscape setting. Species richness and bat activity increased significantly with higher forest land cover in the 200 m buffer and at smaller distance to forested areas. Moreover, species richness increased in proximity to tree groves. Larger amount of forest land cover and smaller distance to forest also resulted in a higher activity of bats on grassland sites in the beginning of the year during May, June and July. Landscape elements near grassland sites also influenced species composition of bats and species richness of functional groups (open, edge and narrow space foragers). Our results highlight the importance of forested areas, and suggest that agricultural grasslands that are closer to forest remnants might be better buffered against outbreaks of agricultural pest insects due to higher species richness and higher bat activity. Furthermore, our data reveals that even for highly mobile species such as bats, a very dense network of connecting elements within the landscape is

  16. The Importance of Landscape Elements for Bat Activity and Species Richness in Agricultural Areas

    PubMed Central

    Heim, Olga; Treitler, Julia T.; Tschapka, Marco; Knörnschild, Mirjam; Jung, Kirsten

    2015-01-01

    Landscape heterogeneity is regarded as a key factor for maintaining biodiversity and ecosystem function in production landscapes. We investigated whether grassland sites at close vicinity to forested areas are more frequently used by bats. Considering that bats are important consumers of herbivorous insects, including agricultural pest, this is important for sustainable land management. Bat activity and species richness were assessed using repeated monitoring from May to September in 2010 with acoustic monitoring surveys on 50 grassland sites in the Biosphere Reserve Schorfheide-Chorin (North-East Germany). Using spatial analysis (GIS), we measured the closest distance of each grassland site to potentially connecting landscape elements (e.g., trees, linear vegetation, groves, running and standing water). In addition, we assessed the distance to and the percent land cover of forest remnants and urban areas in a 200 m buffer around the recording sites to address differences in the local landscape setting. Species richness and bat activity increased significantly with higher forest land cover in the 200 m buffer and at smaller distance to forested areas. Moreover, species richness increased in proximity to tree groves. Larger amount of forest land cover and smaller distance to forest also resulted in a higher activity of bats on grassland sites in the beginning of the year during May, June and July. Landscape elements near grassland sites also influenced species composition of bats and species richness of functional groups (open, edge and narrow space foragers). Our results highlight the importance of forested areas, and suggest that agricultural grasslands that are closer to forest remnants might be better buffered against outbreaks of agricultural pest insects due to higher species richness and higher bat activity. Furthermore, our data reveals that even for highly mobile species such as bats, a very dense network of connecting elements within the landscape is

  17. Perceptions of Vocational Agriculture Instructors Regarding Knowledge and Importance of Including Selected Agricultural Mechanics Units in the Vocational Agriculture Program.

    ERIC Educational Resources Information Center

    Heimgartner, Dale C.; Foster, Richard M.

    1981-01-01

    A survey of teachers in five northwestern states revealed that respondents in all states rated the units of arc welding and oxyacetylene welding as the most important units to be included in secondary vocational agriculture programs. (LRA)

  18. Non-bee insects are important contributors to global crop pollination

    PubMed Central

    Bartomeus, Ignasi; Garibaldi, Lucas A.; Garratt, Michael P. D.; Howlett, Brad G.; Winfree, Rachael; Cunningham, Saul A.; Mayfield, Margaret M.; Arthur, Anthony D.; Andersson, Georg K. S.; Bommarco, Riccardo; Brittain, Claire; Carvalheiro, Luísa G.; Chacoff, Natacha P.; Entling, Martin H.; Foully, Benjamin; Freitas, Breno M.; Gemmill-Herren, Barbara; Ghazoul, Jaboury; Griffin, Sean R.; Gross, Caroline L.; Herbertsson, Lina; Herzog, Felix; Hipólito, Juliana; Jaggar, Sue; Jauker, Frank; Klein, Alexandra-Maria; Kleijn, David; Krishnan, Smitha; Lemos, Camila Q.; Lindström, Sandra A. M.; Mandelik, Yael; Monteiro, Victor M.; Nelson, Warrick; Nilsson, Lovisa; Pattemore, David E.; de O. Pereira, Natália; Pisanty, Gideon; Potts, Simon G.; Reemer, Menno; Rundlöf, Maj; Sheffield, Cory S.; Scheper, Jeroen; Schüepp, Christof; Smith, Henrik G.; Stanley, Dara A.; Stout, Jane C.; Szentgyörgyi, Hajnalka; Taki, Hisatomo; Vergara, Carlos H.; Viana, Blandina F.; Woyciechowski, Michal

    2016-01-01

    Wild and managed bees are well documented as effective pollinators of global crops of economic importance. However, the contributions by pollinators other than bees have been little explored despite their potential to contribute to crop production and stability in the face of environmental change. Non-bee pollinators include flies, beetles, moths, butterflies, wasps, ants, birds, and bats, among others. Here we focus on non-bee insects and synthesize 39 field studies from five continents that directly measured the crop pollination services provided by non-bees, honey bees, and other bees to compare the relative contributions of these taxa. Non-bees performed 25–50% of the total number of flower visits. Although non-bees were less effective pollinators than bees per flower visit, they made more visits; thus these two factors compensated for each other, resulting in pollination services rendered by non-bees that were similar to those provided by bees. In the subset of studies that measured fruit set, fruit set increased with non-bee insect visits independently of bee visitation rates, indicating that non-bee insects provide a unique benefit that is not provided by bees. We also show that non-bee insects are not as reliant as bees on the presence of remnant natural or seminatural habitat in the surrounding landscape. These results strongly suggest that non-bee insect pollinators play a significant role in global crop production and respond differently than bees to landscape structure, probably making their crop pollination services more robust to changes in land use. Non-bee insects provide a valuable service and provide potential insurance against bee population declines. PMID:26621730

  19. Non-bee insects are important contributors to global crop pollination.

    PubMed

    Rader, Romina; Bartomeus, Ignasi; Garibaldi, Lucas A; Garratt, Michael P D; Howlett, Brad G; Winfree, Rachael; Cunningham, Saul A; Mayfield, Margaret M; Arthur, Anthony D; Andersson, Georg K S; Bommarco, Riccardo; Brittain, Claire; Carvalheiro, Luísa G; Chacoff, Natacha P; Entling, Martin H; Foully, Benjamin; Freitas, Breno M; Gemmill-Herren, Barbara; Ghazoul, Jaboury; Griffin, Sean R; Gross, Caroline L; Herbertsson, Lina; Herzog, Felix; Hipólito, Juliana; Jaggar, Sue; Jauker, Frank; Klein, Alexandra-Maria; Kleijn, David; Krishnan, Smitha; Lemos, Camila Q; Lindström, Sandra A M; Mandelik, Yael; Monteiro, Victor M; Nelson, Warrick; Nilsson, Lovisa; Pattemore, David E; Pereira, Natália de O; Pisanty, Gideon; Potts, Simon G; Reemer, Menno; Rundlöf, Maj; Sheffield, Cory S; Scheper, Jeroen; Schüepp, Christof; Smith, Henrik G; Stanley, Dara A; Stout, Jane C; Szentgyörgyi, Hajnalka; Taki, Hisatomo; Vergara, Carlos H; Viana, Blandina F; Woyciechowski, Michal

    2016-01-01

    Wild and managed bees are well documented as effective pollinators of global crops of economic importance. However, the contributions by pollinators other than bees have been little explored despite their potential to contribute to crop production and stability in the face of environmental change. Non-bee pollinators include flies, beetles, moths, butterflies, wasps, ants, birds, and bats, among others. Here we focus on non-bee insects and synthesize 39 field studies from five continents that directly measured the crop pollination services provided by non-bees, honey bees, and other bees to compare the relative contributions of these taxa. Non-bees performed 25-50% of the total number of flower visits. Although non-bees were less effective pollinators than bees per flower visit, they made more visits; thus these two factors compensated for each other, resulting in pollination services rendered by non-bees that were similar to those provided by bees. In the subset of studies that measured fruit set, fruit set increased with non-bee insect visits independently of bee visitation rates, indicating that non-bee insects provide a unique benefit that is not provided by bees. We also show that non-bee insects are not as reliant as bees on the presence of remnant natural or seminatural habitat in the surrounding landscape. These results strongly suggest that non-bee insect pollinators play a significant role in global crop production and respond differently than bees to landscape structure, probably making their crop pollination services more robust to changes in land use. Non-bee insects provide a valuable service and provide potential insurance against bee population declines. PMID:26621730

  20. Insect Pollinated Crops, Insect Pollinators and US Agriculture: Trend Analysis of Aggregate Data for the Period 1992–2009

    PubMed Central

    Calderone, Nicholas W.

    2012-01-01

    In the US, the cultivated area (hectares) and production (tonnes) of crops that require or benefit from insect pollination (directly dependent crops: apples, almonds, blueberries, cucurbits, etc.) increased from 1992, the first year in this study, through 1999 and continued near those levels through 2009; aggregate yield (tonnes/hectare) remained unchanged. The value of directly dependent crops attributed to all insect pollination (2009 USD) decreased from $14.29 billion in 1996, the first year for value data in this study, to $10.69 billion in 2001, but increased thereafter, reaching $15.12 billion by 2009. The values attributed to honey bees and non-Apis pollinators followed similar patterns, reaching $11.68 billion and $3.44 billion, respectively, by 2009. The cultivated area of crops grown from seeds resulting from insect pollination (indirectly dependent crops: legume hays, carrots, onions, etc.) was stable from 1992 through 1999, but has since declined. Production of those crops also declined, albeit not as rapidly as the decline in cultivated area; this asymmetry was due to increases in aggregate yield. The value of indirectly dependent crops attributed to insect pollination declined from $15.45 billion in 1996 to $12.00 billion in 2004, but has since trended upward. The value of indirectly dependent crops attributed to honey bees and non-Apis pollinators, exclusive of alfalfa leafcutter bees, has declined since 1996 to $5.39 billion and $1.15 billion, respectively in 2009. The value of alfalfa hay attributed to alfalfa leafcutter bees ranged between $4.99 and $7.04 billion. Trend analysis demonstrates that US producers have a continued and significant need for insect pollinators and that a diminution in managed or wild pollinator populations could seriously threaten the continued production of insect pollinated crops and crops grown from seeds resulting from insect pollination. PMID:22629374

  1. Insect pollinated crops, insect pollinators and US agriculture: trend analysis of aggregate data for the period 1992-2009.

    PubMed

    Calderone, Nicholas W

    2012-01-01

    In the US, the cultivated area (hectares) and production (tonnes) of crops that require or benefit from insect pollination (directly dependent crops: apples, almonds, blueberries, cucurbits, etc.) increased from 1992, the first year in this study, through 1999 and continued near those levels through 2009; aggregate yield (tonnes/hectare) remained unchanged. The value of directly dependent crops attributed to all insect pollination (2009 USD) decreased from $14.29 billion in 1996, the first year for value data in this study, to $10.69 billion in 2001, but increased thereafter, reaching $15.12 billion by 2009. The values attributed to honey bees and non-Apis pollinators followed similar patterns, reaching $11.68 billion and $3.44 billion, respectively, by 2009. The cultivated area of crops grown from seeds resulting from insect pollination (indirectly dependent crops: legume hays, carrots, onions, etc.) was stable from 1992 through 1999, but has since declined. Production of those crops also declined, albeit not as rapidly as the decline in cultivated area; this asymmetry was due to increases in aggregate yield. The value of indirectly dependent crops attributed to insect pollination declined from $15.45 billion in 1996 to $12.00 billion in 2004, but has since trended upward. The value of indirectly dependent crops attributed to honey bees and non-Apis pollinators, exclusive of alfalfa leafcutter bees, has declined since 1996 to $5.39 billion and $1.15 billion, respectively in 2009. The value of alfalfa hay attributed to alfalfa leafcutter bees ranged between $4.99 and $7.04 billion. Trend analysis demonstrates that US producers have a continued and significant need for insect pollinators and that a diminution in managed or wild pollinator populations could seriously threaten the continued production of insect pollinated crops and crops grown from seeds resulting from insect pollination. PMID:22629374

  2. Phylogeny of economically important insect pests that infesting several crops species in Malaysia

    NASA Astrophysics Data System (ADS)

    Ghazali, Siti Zafirah; Zain, Badrul Munir Md.; Yaakop, Salmah

    2014-09-01

    This paper reported molecular data on insect pests of commercial crops in Peninsular Malaysia. Fifteen insect pests (Metisa plana, Calliteara horsefeldii, Cotesia vestalis, Bactrocera papayae, Bactrocera carambolae, Bactrocera latifrons, Conopomorpha cramella, Sesamia inferens, Chilo polychrysa, Rhynchophorus vulneratus, and Rhynchophorus ferrugineus) of nine crops were sampled (oil palm, coconut, paddy, cocoa, starfruit, angled loofah, guava, chili and mustard) and also four species that belong to the fern's pest (Herpetogramma platycapna) and storage and rice pests (Tribolium castaneum, Oryzaephilus surinamensis and Cadra cautella). The presented phylogeny summarized the initial phylogenetic hypothesis, which concerning by implementation of the economically important insect pests. In this paper, phylogenetic relationships among 39 individuals of 15 species that belonging to three orders under 12 genera were inferred from DNA sequences of mitochondrial marker, cytochrome oxidase subunit I (COI) and nuclear marker, ribosomal DNA 28S D2 region. The phylogenies resulted from the phylogenetic analyses of both genes are relatively similar, but differ in the sequence of evolution. Interestingly, this most recent molecular data of COI sequences data by using Bayesian Inference analysis resulted a more-resolved phylogeny that corroborated with traditional hypotheses of holometabolan relationships based on traditional hypotheses of holometabolan relationships and most of recently molecular study compared to 28S sequences. This finding provides the information on relationships of pests species, which infested several crops in Malaysia and also estimation on Holometabola's order relationships. The identification of the larval stages of insect pests could be done accurately, without waiting the emergence of adults and supported by the phylogenetic tree.

  3. Importance of Animals in Agricultural Sustainability and Food Security.

    PubMed

    Reynolds, Lawrence P; Wulster-Radcliffe, Meghan C; Aaron, Debra K; Davis, Teresa A

    2015-07-01

    A conservative projection shows the world's population growing by 32% (to 9.5 billion) by 2050 and 53% (to 11 billion) by 2100 compared with its current level of 7.2 billion. Because most arable land worldwide is already in use, and water and energy also are limiting, increased production of food will require a substantial increase in efficiency. In this article, we highlight the importance of animals to achieving food security in terms of their valuable contributions to agricultural sustainability, especially in developing countries, and the high nutritional value of animal products in the diet. PMID:25972529

  4. Importance of incorporating agriculture in conceptual rainfall-runoff models

    NASA Astrophysics Data System (ADS)

    de Boer-Euser, Tanja; Hrachowitz, Markus; Winsemius, Hessel; Savenije, Hubert

    2016-04-01

    Incorporating spatially variable information is a frequently discussed option to increase the performance of (semi-)distributed conceptual rainfall-runoff models. One of the methods to do this is by using this spatially variable information to delineate Hydrological Response Units (HRUs) within a catchment. In large parts of Europe the original forested land cover is replaced by an agricultural land cover. This change in land cover probably affects the dominant runoff processes in the area, for example by increasing the Hortonian overland flow component, especially on the flatter and higher elevated parts of the catchment. A change in runoff processes implies a change in HRUs as well. A previous version of our model distinguished wetlands (areas close to the stream) from the remainder of the catchment. However, this configuration was not able to reproduce all fast runoff processes, both in summer as in winter. Therefore, this study tests whether the reproduction of fast runoff processes can be improved by incorporating a HRU which explicitly accounts for the effect of agriculture. A case study is carried out in the Ourthe catchment in Belgium. For this case study the relevance of different process conceptualisations is tested stepwise. Among the conceptualisations are Hortonian overland flow in summer and winter, reduced infiltration capacity due to a partly frozen soil and the relative effect of rainfall and snow smelt in case of this frozen soil. The results show that the named processes can make a large difference on event basis, especially the Hortonian overland flow in summer and the combination of rainfall and snow melt on (partly) frozen soil in winter. However, differences diminish when the modelled period of several years is evaluated based on standard metrics like Nash-Sutcliffe Efficiency. These results emphasise on one hand the importance of incorporating the effects of agricultural in conceptual models and on the other hand the importance of more event

  5. The importance of water for the mechanical properties of insect cuticle

    NASA Astrophysics Data System (ADS)

    Klocke, D.; Schmitz, H.

    2011-04-01

    Insect cuticle has a broad range of mechanical properties. As it has to provide a very efficient and lightweight skeleton, cuticle is a highly interesting composite-material and may serve as a natural model for new biomimetic materials. However, the water content of insect cuticle is of great importance for its material properties. Here, we present a new method to perform nano-indentation experiments in cuticle which has its full water content. Parts of the exoskeleton of Locusta migratoria were investigated to determine the elastic modulus (Er) and hardness (H) of the cuticle. Cuticle sections were measured in air and then submerged and measured in water. As insect cuticle is an anisotropic material, we performed nano-indentation in the normal as well as in the transverse direction and also tested different cuticle layers within each sample (exo-, meso- and endo-cuticle). It turned out that a change of the water content has a dramatic impact on the material properties of the cuticle. For example, the Er of submerged endo-cuticle turned out to be 75% lower than of endo-cuticle samples measured in air. Further, the proportion of material property values between different cuticle layers within a sample change dramatically after addition of water.

  6. Large-scale pollination experiment demonstrates the importance of insect pollination in winter oilseed rape.

    PubMed

    Lindström, Sandra A M; Herbertsson, Lina; Rundlöf, Maj; Smith, Henrik G; Bommarco, Riccardo

    2016-03-01

    Insect pollination, despite its potential to contribute substantially to crop production, is not an integrated part of agronomic planning. A major reason for this are knowledge gaps in the contribution of pollinators to yield, which partly result from difficulties in determining area-based estimates of yield effects from insect pollination under field conditions. We have experimentally manipulated honey bee Apis mellifera densities at 43 oilseed rape Brassica napus fields over 2 years in Scandinavia. Honey bee hives were placed in 22 fields; an additional 21 fields without large apiaries in the surrounding landscape were selected as controls. Depending on the pollination system in the parental generation, the B. napus cultivars in the crop fields are classified as either open-pollinated or first-generation hybrids, with both types being open-pollinated in the generation of plants cultivated in the fields. Three cultivars of each type were grown. We measured the activity of flower-visiting insects during flowering and estimated yields by harvesting with small combine harvesters. The addition of honey bee hives to the fields dramatically increased abundance of flower-visiting honey bees in those fields. Honey bees affected yield, but the effect depended on cultivar type (p = 0.04). Post-hoc analysis revealed that open-pollinated cultivars, but not hybrid cultivars, had 11% higher yields in fields with added honey bees than those grown in the control fields (p = 0.07). To our knowledge, this is the first whole-field study in replicated landscapes to assess the benefit of insect pollination in oilseed rape. Our results demonstrate that honey bees have the potential to increase oilseed rape yields, thereby emphasizing the importance of pollinator management for optimal cultivation of oilseed rape. PMID:26650584

  7. Biodiversity of Aspergillus species in some important agricultural products

    PubMed Central

    Perrone, G.; Susca, A.; Cozzi, G.; Ehrlich, K.; Varga, J.; Frisvad, J.C.; Meijer, M.; Noonim, P.; Mahakarnchanakul, W.; Samson, R.A.

    2007-01-01

    The genus Aspergillus is one of the most important filamentous fungal genera. Aspergillus species are used in the fermentation industry, but they are also responsible of various plant and food secondary rot, with the consequence of possible accumulation of mycotoxins. The aflatoxin producing A. flavus and A. parasiticus, and ochratoxinogenic A. niger, A. ochraceus and A. carbonarius species are frequently encountered in agricultural products. Studies on the biodiversity of toxigenic Aspergillus species is useful to clarify molecular, ecological and biochemical characteristics of the different species in relation to their different adaptation to environmental and geographical conditions, and to their potential toxigenicity. Here we analyzed the biodiversity of ochratoxin producing species occurring on two important crops: grapes and coffee, and the genetic diversity of A. flavus populations occurring in agricultural fields. Altogether nine different black Aspergillus species can be found on grapes which are often difficult to identify with classical methods. The polyphasic approach used in our studies led to the identification of three new species occurring on grapes: A. brasiliensis, A. ibericus, and A. uvarum. Similar studies on the Aspergillus species occurring on coffee beans have evidenced in the last five years that A. carbonarius is an important source of ochratoxin A in coffee. Four new species within the black aspergilli were also identified in coffee beans: A. sclerotioniger, A. lacticoffeatus, A. sclerotiicarbonarius, and A. aculeatinus. The genetic diversity within A. flavus populations has been widely studied in relation to their potential aflatoxigenicity and morphological variants L- and S-strains. Within A. flavus and other Aspergillus species capable of aflatoxin production, considerable diversity is found. We summarise the main recent achievements in the diversity of the aflatoxin gene cluster in A. flavus populations, A. parasiticus and the non

  8. 7 CFR 319.8-20 - Importations by the Department of Agriculture.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 5 2012-01-01 2012-01-01 false Importations by the Department of Agriculture. 319.8-20 Section 319.8-20 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Foreign Cotton...

  9. 7 CFR 319.8-20 - Importations by the Department of Agriculture.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 5 2013-01-01 2013-01-01 false Importations by the Department of Agriculture. 319.8-20 Section 319.8-20 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Foreign Cotton...

  10. The MrCYP52 cytochrome P450 monoxygenase gene of Metarhizium robertsii is important for utilizing insect epicuticular hydrocarbons.

    PubMed

    Lin, Liangcai; Fang, Weiguo; Liao, Xinggang; Wang, Fengqing; Wei, Dongzhi; St Leger, Raymond J

    2011-01-01

    Fungal pathogens of plants and insects infect their hosts by direct penetration of the cuticle. Plant and insect cuticles are covered by a hydrocarbon-rich waxy outer layer that represents the first barrier against infection. However, the fungal genes that underlie insect waxy layer degradation have received little attention. Here we characterize the single cytochrome P450 monoxygenase family 52 (MrCYP52) gene of the insect pathogen Metarhizium robertsii, and demonstrate that it encodes an enzyme required for efficient utilization of host hydrocarbons. Expressing a green florescent protein gene under control of the MrCYP52 promoter confirmed that MrCYP52 is up regulated on insect cuticle as well as by artificial media containing decane (C10), extracted cuticle hydrocarbons, and to a lesser extent long chain alkanes. Disrupting MrCYP52 resulted in reduced growth on epicuticular hydrocarbons and delayed developmental processes on insect cuticle, including germination and production of appressoria (infection structures). Extraction of alkanes from cuticle prevented induction of MrCYP52 and reduced growth. Insect bioassays against caterpillars (Galleria mellonella) confirmed that disruption of MrCYP52 significantly reduces virulence. However, MrCYP52 was dispensable for normal germination and appressorial formation in vitro when the fungus was supplied with nitrogenous nutrients. We conclude therefore that MrCYP52 mediates degradation of epicuticular hydrocarbons and these are an important nutrient source, but not a source of chemical signals that trigger infection processes. PMID:22194968

  11. Daily Evolution of the Insect Biomass Spectrum in an Agricultural Landscape Accessed with Lidar

    NASA Astrophysics Data System (ADS)

    Brydegaard, Mikkel; Gebru, Alem; Kirkeby, Carsten; Åkesson, Susanne; Smith, Henrik

    2016-06-01

    We present measurements of atmospheric insect fauna intercepted by a static lidar transect over arable and pastoral land over one day. We observe nearly a quarter million of events which are calibrated to optical cross section. Biomass spectra are derived from the size distribution and presented against space and time. We discuss detection limits and instrument biasing, and we relate the insect observations to relevant ecological landscape features and land use. Future directions and improvements of the technique are also outlined.

  12. ChiloDB: a genomic and transcriptome database for an important rice insect pest Chilo suppressalis

    PubMed Central

    Yin, Chuanlin; Liu, Ying; Liu, Jinding; Xiao, Huamei; Huang, Shuiqing; Lin, Yongjun; Han, Zhaojun; Li, Fei

    2014-01-01

    ChiloDB is an integrated resource that will be of use to the rice stem borer research community. The rice striped stem borer (SSB), Chilo suppressalis Walker, is a major rice pest that causes severe yield losses in most rice-producing countries. A draft genome of this insect is available. The aims of ChiloDB are (i) to store recently acquired genomic sequence and transcriptome data and integrate them with protein-coding genes, microRNAs, piwi-interacting RNAs (piRNAs) and RNA sequencing (RNA-Seq) data and (ii) to provide comprehensive search tools and downloadable data sets for comparative genomics and gene annotation of this important rice pest. ChiloDB contains the first version of the official SSB gene set, comprising 80 479 scaffolds and 10 221 annotated protein-coding genes. Additionally, 262 SSB microRNA genes predicted from a small RNA library, 82 639 piRNAs identified using the piRNApredictor software, 37 040 transcripts from a midgut transcriptome and 69 977 transcripts from a mixed sample have all been integrated into ChiloDB. ChiloDB was constructed using a data structure that is compatible with data resources, which will be incorporated into the database in the future. This resource will serve as a long-term and open-access database for research on the biology, evolution and pest control of SSB. To the best of our knowledge, ChiloDB is one of the first genomic and transcriptome database for rice insect pests. Database URL: http://ento.njau.edu.cn/ChiloDB. PMID:24997141

  13. ChiloDB: a genomic and transcriptome database for an important rice insect pest Chilo suppressalis.

    PubMed

    Yin, Chuanlin; Liu, Ying; Liu, Jinding; Xiao, Huamei; Huang, Shuiqing; Lin, Yongjun; Han, Zhaojun; Li, Fei

    2014-01-01

    ChiloDB is an integrated resource that will be of use to the rice stem borer research community. The rice striped stem borer (SSB), Chilo suppressalis Walker, is a major rice pest that causes severe yield losses in most rice-producing countries. A draft genome of this insect is available. The aims of ChiloDB are (i) to store recently acquired genomic sequence and transcriptome data and integrate them with protein-coding genes, microRNAs, piwi-interacting RNAs (piRNAs) and RNA sequencing (RNA-Seq) data and (ii) to provide comprehensive search tools and downloadable data sets for comparative genomics and gene annotation of this important rice pest. ChiloDB contains the first version of the official SSB gene set, comprising 80,479 scaffolds and 10 221 annotated protein-coding genes. Additionally, 262 SSB microRNA genes predicted from a small RNA library, 82 639 piRNAs identified using the piRNApredictor software, 37,040 transcripts from a midgut transcriptome and 69 977 transcripts from a mixed sample have all been integrated into ChiloDB. ChiloDB was constructed using a data structure that is compatible with data resources, which will be incorporated into the database in the future. This resource will serve as a long-term and open-access database for research on the biology, evolution and pest control of SSB. To the best of our knowledge, ChiloDB is one of the first genomic and transcriptome database for rice insect pests. Database URL: http://ento.njau.edu.cn/ChiloDB. PMID:24997141

  14. History of population fluctuations and infestations of important forest insects in the Queen Charlotte Islands forest district, 1993. FIDS report No. 94-10. Annual publication

    SciTech Connect

    1994-12-31

    This report provides some history of some important forest insects in the District. It serves to designate the species of insects which cause damage; record the pattern of population fluctuations; designate areas that appear to have chronic problems; and point out the possibility of damage in different areas by insects, including species not known to have caused damage in the Queen Charlotte Islands.

  15. Functional haplodiploidy: a mechanism for the spread of insecticide resistance in an important international insect pest.

    PubMed Central

    Brun, L O; Stuart, J; Gaudichon, V; Aronstein, K; French-Constant, R H

    1995-01-01

    The coffee berry borer, Hypothenemus hampei, is the most important insect pest of coffee worldwide and has an unusual life history that ensures a high degree of inbreeding. Individual females lay a predominantly female brood within individual coffee berries and because males are flightless there is almost entirely full sib mating. We investigated the genetics associated with this interesting life history after the important discovery of resistance to the cyclodiene type insecticide endosulfan. Both the inheritance of the resistance phenotype and the resistance-associated point mutation in the gamma-aminobutyric acid receptor gene Rdl were examined. Consistent with haplodiploidy, males failed to express and transmit paternally derived resistance alleles. Furthermore, while cytological examination revealed that males are diploid, one set of chromosomes was condensed, and probably nonfunctional, in the somatic cells of all males examined. Moreover, although two sets of chromosomes were present in primary spermatocytes, the chromosomes failed to pair before the single meiotic division, and only one set was packaged in sperm. Thus, the coffee berry borer is "functionally" haplodiploid. Its genetics and life history may therefore represent an interesting intermediate step in the evolution of true haplodiploidy. The influence of this breeding system on the spread of insecticide resistance is discussed. Images Fig. 1 Fig. 3 Fig. 4 Fig. 5 PMID:7568233

  16. Iowa Commercial Pesticide Applicator Manual, Category 1B: Agricultural Insect Control.

    ERIC Educational Resources Information Center

    Stockdale, Harold J.; Ryan, Stephen O.

    This guide provides basic information to meet specific standards for pesticide applicators. The text is concerned with the control of economic insect pests on field and forage crops, especially corn, soybeans, and alfalfa. Full color photographs of the more destructive pests are provided to aid in identification of problems. Precautions and…

  17. Harmonic radar: efficacy at detecting and recovering insects on agricultural host plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    BACKGROUND: In pest management research, harmonic radar systems have been largely used to study insect movement across open or vegetation poor areas, because the microwave signal is attenuated by the high water content of vegetation. This study aimed at determining whether the efficacy of the tech...

  18. Ecdysteroids and Juvenile Hormones of Whiteflies, Important Insect Vectors for Plant Viruses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Ecdysteroids and juvenile hormones (JHs) regulate many physiological events throughout the insect life cycle, including molting, metamorphosis, ecdysis, diapause, reproduction and behavior. Fluctuation of whitefly ecdysteroid levels and the identity of the whitefly molting hormone have only been re...

  19. Rapid genetic turnover in populations of the insect pest Bemisia tabaci Middle East: Asia Minor 1 in an agricultural landscape.

    PubMed

    Dinsdale, A; Schellhorn, N A; De Barro, P; Buckley, Y M; Riginos, C

    2012-10-01

    Organisms differ greatly in dispersal ability, and landscapes differ in amenability to an organism's movement. Thus, landscape structure and heterogeneity can affect genetic composition of populations. While many agricultural pests are known for their ability to disperse rapidly, it is unclear how fast and over what spatial scale insect pests might respond to the temporally dynamic agricultural landscapes they inhabit. We used population genetic analyses of a severe crop pest, a member of the Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoidea: Aleyrodidea) cryptic species complex known as Middle East-Asia Minor 1 (commonly known as biotype B), to estimate spatial and temporal genetic diversity over four months of the 2006-2007 summer growing season. We examined 559 individuals from eight sites, which were scored for eight microsatellite loci. Temporal genetic structure greatly exceeded spatial structure. There was significant temporal change in local genetic composition from the beginning to the end of the season accompanied by heterozygote deficits and inbreeding. This temporal structure suggests entire cohorts of pests can occupy a large and variable agricultural landscape but are rapidly replaced. These rapid genetic fluctuations reinforce the concept that agricultural landscapes are dynamic mosaics in time and space and may contribute to better decisions for pest and insecticide resistance management. PMID:22420748

  20. Where is the UK's pollinator biodiversity? The importance of urban areas for flower-visiting insects

    PubMed Central

    Baldock, Katherine C. R.; Goddard, Mark A.; Hicks, Damien M.; Kunin, William E.; Mitschunas, Nadine; Osgathorpe, Lynne M.; Potts, Simon G.; Robertson, Kirsty M.; Scott, Anna V.; Stone, Graham N.; Vaughan, Ian P.; Memmott, Jane

    2015-01-01

    Insect pollinators provide a crucial ecosystem service, but are under threat. Urban areas could be important for pollinators, though their value relative to other habitats is poorly known. We compared pollinator communities using quantified flower-visitation networks in 36 sites (each 1 km2) in three landscapes: urban, farmland and nature reserves. Overall, flower-visitor abundance and species richness did not differ significantly between the three landscape types. Bee abundance did not differ between landscapes, but bee species richness was higher in urban areas than farmland. Hoverfly abundance was higher in farmland and nature reserves than urban sites, but species richness did not differ significantly. While urban pollinator assemblages were more homogeneous across space than those in farmland or nature reserves, there was no significant difference in the numbers of rarer species between the three landscapes. Network-level specialization was higher in farmland than urban sites. Relative to other habitats, urban visitors foraged from a greater number of plant species (higher generality) but also visited a lower proportion of available plant species (higher specialization), both possibly driven by higher urban plant richness. Urban areas are growing, and improving their value for pollinators should be part of any national strategy to conserve and restore pollinators. PMID:25673686

  1. Lucifensins, the Insect Defensins of Biomedical Importance: The Story behind Maggot Therapy

    PubMed Central

    Čeřovský, Václav; Bém, Robert

    2014-01-01

    Defensins are the most widespread antimicrobial peptides characterised in insects. These cyclic peptides, 4–6 kDa in size, are folded into α-helical/β-sheet mixed structures and have a common conserved motif of three intramolecular disulfide bridges with a Cys1-Cys4, Cys2-Cys5 and Cys3-Cys6 connectivity. They have the ability to kill especially Gram-positive bacteria and some fungi, but Gram-negative bacteria are more resistant against them. Among them are the medicinally important compounds lucifensin and lucifensin II, which have recently been identified in the medicinal larvae of the blowflies Lucilia sericata and Lucilia cuprina, respectively. These defensins contribute to wound healing during a procedure known as maggot debridement therapy (MDT) which is routinely used at hospitals worldwide. Here we discuss the decades-long story of the effort to isolate and characterise these two defensins from the bodies of medicinal larvae or from their secretions/excretions. Furthermore, our previous studies showed that the free-range larvae of L. sericata acutely eliminated most of the Gram-positive strains of bacteria and some Gram-negative strains in patients with infected diabetic foot ulcers, but MDT was ineffective during the healing of wounds infected with Pseudomonas sp. and Acinetobacter sp. The bactericidal role of lucifensins secreted into the infected wound by larvae during MDT and its ability to enhance host immunity by functioning as immunomodulator is also discussed. PMID:24583934

  2. Lucifensins, the Insect Defensins of Biomedical Importance: The Story behind Maggot Therapy.

    PubMed

    Ceřovský, Václav; Bém, Robert

    2014-01-01

    Defensins are the most widespread antimicrobial peptides characterised in insects. These cyclic peptides, 4-6 kDa in size, are folded into α-helical/β-sheet mixed structures and have a common conserved motif of three intramolecular disulfide bridges with a Cys1-Cys4, Cys2-Cys5 and Cys3-Cys6 connectivity. They have the ability to kill especially Gram-positive bacteria and some fungi, but Gram-negative bacteria are more resistant against them. Among them are the medicinally important compounds lucifensin and lucifensin II, which have recently been identified in the medicinal larvae of the blowflies Lucilia sericata and Lucilia cuprina, respectively. These defensins contribute to wound healing during a procedure known as maggot debridement therapy (MDT) which is routinely used at hospitals worldwide. Here we discuss the decades-long story of the effort to isolate and characterise these two defensins from the bodies of medicinal larvae or from their secretions/excretions. Furthermore, our previous studies showed that the free-range larvae of L. sericata acutely eliminated most of the Gram-positive strains of bacteria and some Gram-negative strains in patients with infected diabetic foot ulcers, but MDT was ineffective during the healing of wounds infected with Pseudomonas sp. and Acinetobacter sp. The bactericidal role of lucifensins secreted into the infected wound by larvae during MDT and its ability to enhance host immunity by functioning as immunomodulator is also discussed. PMID:24583934

  3. Oligocene Termite Nests with In Situ Fungus Gardens from the Rukwa Rift Basin, Tanzania, Support a Paleogene African Origin for Insect Agriculture

    PubMed Central

    Roberts, Eric M.; Todd, Christopher N.; Aanen, Duur K.; Nobre, Tânia; Hilbert-Wolf, Hannah L.; O’Connor, Patrick M.; Tapanila, Leif; Mtelela, Cassy; Stevens, Nancy J.

    2016-01-01

    Based on molecular dating, the origin of insect agriculture is hypothesized to have taken place independently in three clades of fungus-farming insects: the termites, ants or ambrosia beetles during the Paleogene (66–24 Ma). Yet, definitive fossil evidence of fungus-growing behavior has been elusive, with no unequivocal records prior to the late Miocene (7–10 Ma). Here we report fossil evidence of insect agriculture in the form of fossil fungus gardens, preserved within 25 Ma termite nests from southwestern Tanzania. Using these well-dated fossil fungus gardens, we have recalibrated molecular divergence estimates for the origins of termite agriculture to around 31 Ma, lending support to hypotheses suggesting an African Paleogene origin for termite-fungus symbiosis; perhaps coinciding with rift initiation and changes in the African landscape. PMID:27333288

  4. Oligocene Termite Nests with In Situ Fungus Gardens from the Rukwa Rift Basin, Tanzania, Support a Paleogene African Origin for Insect Agriculture.

    PubMed

    Roberts, Eric M; Todd, Christopher N; Aanen, Duur K; Nobre, Tânia; Hilbert-Wolf, Hannah L; O'Connor, Patrick M; Tapanila, Leif; Mtelela, Cassy; Stevens, Nancy J

    2016-01-01

    Based on molecular dating, the origin of insect agriculture is hypothesized to have taken place independently in three clades of fungus-farming insects: the termites, ants or ambrosia beetles during the Paleogene (66-24 Ma). Yet, definitive fossil evidence of fungus-growing behavior has been elusive, with no unequivocal records prior to the late Miocene (7-10 Ma). Here we report fossil evidence of insect agriculture in the form of fossil fungus gardens, preserved within 25 Ma termite nests from southwestern Tanzania. Using these well-dated fossil fungus gardens, we have recalibrated molecular divergence estimates for the origins of termite agriculture to around 31 Ma, lending support to hypotheses suggesting an African Paleogene origin for termite-fungus symbiosis; perhaps coinciding with rift initiation and changes in the African landscape. PMID:27333288

  5. Relative Importance of Biotic and Abiotic Soil Components to Plant Growth and Insect Herbivore Population Dynamics

    PubMed Central

    Vandegehuchte, Martijn L.; de la Peña, Eduardo; Bonte, Dries

    2010-01-01

    Background Plants are affected by several aspects of the soil, which have the potential to exert cascading effects on the performance of herbivorous insects. The effects of biotic and abiotic soil characteristics have however mostly been investigated in isolation, leaving their relative importance largely unexplored. Such is the case for the dune grass Ammophila, whose decline under decreasing sand accretion is argued to be caused by either biotic or abiotic soil properties. Methodology/Principal Findings By manipulating dune soils from three different regions, we decoupled the contributions of region, the abiotic and biotic soil component to the variation in characteristics of Ammophila arenaria seedlings and Schizaphis rufula aphid populations. Root mass fraction and total dry biomass of plants were affected by soil biota, although the latter effect was not consistent across regions. None of the measured plant properties were significantly affected by the abiotic soil component. Aphid population characteristics all differed between regions, irrespective of whether soil biota were present or absent. Hence these effects were due to differences in abiotic soil properties between regions. Although several chemical properties of the soil mixtures were measured, none of these were consistent with results for plant or aphid traits. Conclusions/Significance Plants were affected more strongly by soil biota than by abiotic soil properties, whereas the opposite was true for aphids. Our results thus demonstrate that the relative importance of the abiotic and biotic component of soils can differ for plants and their herbivores. The fact that not all effects of soil properties could be detected across regions moreover emphasizes the need for spatial replication in order to make sound conclusions about the generality of aboveground-belowground interactions. PMID:20886078

  6. Integrated pest management: the push-pull approach for controlling insect pests and weeds of cereals, and its potential for other agricultural systems including animal husbandry.

    PubMed

    Hassanali, Ahmed; Herren, Hans; Khan, Zeyaur R; Pickett, John A; Woodcock, Christine M

    2008-02-12

    This paper describes the 'push-pull' or 'stimulo-deterrent diversionary' strategy in relation to current and potential examples from our own experiences. The push-pull effect is established by exploiting semiochemicals to repel insect pests from the crop ('push') and to attract them into trap crops ('pull'). The systems exemplified here have been developed for subsistence farming in Africa and delivery of the semiochemicals is entirely by companion cropping, i.e. intercropping for the push and trap cropping for the pull. The main target was a series of lepidopterous pests attacking maize and other cereals. Although the area given to the cereal crop itself is reduced under the push-pull system, higher yields are produced per unit area. An important spin-off from the project is that the companion crops are valuable forage for farm animals. Leguminous intercrops also provide advantages with regard to plant nutrition and some of the trap crops help with water retention and in reducing land erosion. A major benefit is that certain intercrop plants provide dramatic control of the African witchweed (striga). Animal husbandry forms an essential part of intensive subsistence agriculture in Africa and developments using analogous push-pull control strategies for insect pests of cattle are exemplified. PMID:17652071

  7. Biodiversity of Aspergillus Species in Some Important Agricultural Products

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The genus Aspergillus is one of the most important filamentous fungal genera. Aspergillus species are used in the fermentation industry, but they are also responsible of various plant and food secondary rot, with the consequence of possible accumulation of mycotoxins. The aflatoxin-producing A. fl...

  8. Edible insects are the future?

    PubMed

    van Huis, Arnold

    2016-08-01

    The global increase in demand for meat and the limited land area available prompt the search for alternative protein sources. Also the sustainability of meat production has been questioned. Edible insects as an alternative protein source for human food and animal feed are interesting in terms of low greenhouse gas emissions, high feed conversion efficiency, low land use, and their ability to transform low value organic side streams into high value protein products. More than 2000 insect species are eaten mainly in tropical regions. The role of edible insects in the livelihoods and nutrition of people in tropical countries is discussed, but this food source is threatened. In the Western world, there is an increasing interest in edible insects, and examples are given. Insects as feed, in particular as aquafeed, have a large potential. Edible insects have about the same protein content as conventional meat and more PUFA. They may also have some beneficial health effects. Edible insects need to be processed and turned into palatable dishes. Food safety may be affected by toxicity of insects, contamination with pathogens, spoilage during conservation and allergies. Consumer attitude is a major issue in the Western world and a number of strategies are proposed to encourage insect consumption. We discuss research pathways to make insects a viable sector in food and agriculture: an appropriate disciplinary focus, quantifying its importance, comparing its nutritional value to conventional protein sources, environmental benefits, safeguarding food safety, optimising farming, consumer acceptance and gastronomy. PMID:26908196

  9. Use of shaking treatments and pre-harvest sprays of pyrethroid insecticides to reduce risk of yellowjackets and other insects on Christmas trees imported into Hawaii

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insects are commonly found by Hawaii’s quarantine inspectors on Christmas trees imported from the Pacific Northwest. To reduce the risk of importing yellowjacket (Vespula spp.) queens and other insects, an inspection and tree shaking certification program was begun in 1990. From 1993 to 2006, the an...

  10. 7 CFR 51.2290 - Insect injury.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Insect injury. 51.2290 Section 51.2290 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... § 51.2290 Insect injury. Insect injury means that the insect, web, frass or other evidence of...

  11. 7 CFR 51.2290 - Insect injury.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Insect injury. 51.2290 Section 51.2290 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... § 51.2290 Insect injury. Insect injury means that the insect, web, frass or other evidence of...

  12. [Use of an aerosol generator (Guard) to control injurious insects in forestry, agriculture, and medical disinsection].

    PubMed

    Abdraziakov, O N; Ermishev, Iu V; Levkov, P A

    2012-01-01

    The Guard aerosol generator is a universal multioperational device without a field-of-use restriction in the application of permitted chemical and biological substances, by combining the function of a controlled dispersion aerosol generator and a remote small- and large-drop sprayer in one mechanism and can use aerosol pesticides. The drop fractionation range is as follows: 3-50, 50-100, 100-300, and 200-400 microm for aerosol, dead water, small-drop, and large-drop spraying, respectively, with smooth and step control of working liquid drops. Treatment using the Guard generator has been shown to be highly effective against agricultural and forestry pests. This paper describes the advantages of the Guard sprayer over those of the conventional air and ground ones. The long-term use of the Guard generator to control mosquitoes and ticks in the Tyumen region could substantially improve the epidemiological situation of tick-borne infections and protect children's recreation centers from attacks of bloodsuckers. PMID:22774514

  13. Importance of protein quality versus quantity in alternative host plants for a leaf-feeding insect.

    PubMed

    Barbehenn, Raymond V; Niewiadomski, Julie; Kochmanski, Joseph

    2013-09-01

    The nutritional value of alternative host plants for leaf-feeding insects such as caterpillars is commonly measured in terms of protein quantity. However, nutritional value might also depend on the quality of the foliar protein [i.e., the composition of essential amino acids (EAAs)]. A lack of comparative work on the EAA compositions of herbivores and their host plants has hampered the testing of this hypothesis. We tested the "protein quality hypothesis" using the tree-feeding caterpillars of Lymantria dispar (gypsy moth) and two taxonomically unrelated host plants, red oak (Quercus rubra) and sugar maple (Acer saccharum). Because L. dispar has higher fitness on oak than on maple, support for the hypothesis would be found if protein were of higher quality from oak than from maple. The whole-body EAA composition of L. dispar larvae was measured to estimate its optimum dietary protein composition, which was compared with the EAA compositions of oak and maple leaves. Contrary to the protein quality hypothesis, the EAA compositions of oak and maple were not significantly different in the spring. The growth-limiting EAAs in both tree species were histidine and methionine. Similar results were observed in the summer, with the exception that the histidine composition of oak was between 10 and 15 % greater than in maple leaves. The two main factors that affected the nutritional value of protein from the tree species were the quantities of EAAs, which were consistently higher in oak, and the efficiency of EAA utilization, which decreased from 80 % in May to <50 % in August. We conclude that the relative nutritional value of red oak and sugar maple for L. dispar is more strongly affected by protein quantity than quality. Surveys of many wild herbaceous species also suggest that leaf-feeding insects would be unlikely to specialize on plants based on protein quality. PMID:23297046

  14. Insect Infestations Linked to Shifts in Microclimate: Important Climate Change Implications

    SciTech Connect

    Classen, Aimee T; Hart, Stephen C; Whitham, Thomas G; Cobb, Neil S; Koch, George W

    2005-01-01

    Changes in vegetation due to drought-influenced herbivory may influence microclimate in ecosystems. In combination with studies of insect resistant and susceptible trees, we used long-term herbivore removal experiments with two herbivores of pinon (Pinus edulis Endelm.) to test the general hypothesis that herbivore alteration of plant architecture affects soil microclimate, a major driver of ecosystem-level processes. The pinon needle scale (Matsucoccus acalyptus, Herbert) attacks needles of juvenile trees causing them to develop an open crown. In contrast, the stem-boring moth (Dioryctria albovittella Hulst.) kills the terminal shoots of mature trees, causing the crown to develop a dense form. Our studies focused on how the microclimate effects of these architectural changes are likely to accumulate over time. Three patterns emerged: (1) scale herbivory reduced leaf area index (LAI) of susceptible trees by 39%, whereas moths had no effect on LAI; (2) scale herbivory increased soil moisture and temperature beneath susceptible trees by 35 and 26%, respectively, whereas moths had no effect; and (3) scale and moth herbivory decreased crown interception of precipitation by 51 and 29%, respectively. From these results, we conclude: (1) the magnitude of scale effects on soil moisture and temperature is large, similar to global change scenarios, and sufficient to drive changes in ecosystem processes. (2) The larger sizes of moth-susceptible trees apparently buffered them from most microclimate effects of herbivory, despite marked changes in crown architecture. (3) The phenotypic expression of susceptibility or resistance to scale insects extends beyond plant-herbivore interactions to the physical environment.

  15. Importance and location of instruction for sixty-eight energy competencies for Kansas agriculture

    SciTech Connect

    French, B.T.

    1982-01-01

    The two primary objectives of this study were: to identify which energy competencies had improtance to Kansas agriculture and to determine how appropiate each competency was for three levels of instruction: high school, Area Vocational-Technical School or Community College, or four year college. An energy questionnaire, comprised of 68 competencies, was completed by 140 individuals and provided data to meet the studys primary objectives. Thirty-five returns were recieved from each of four groups: Young Farmers/Young Farm Wives, High School Vo-Ag teachers, Postsecondary AVTS-CC Agricultural teachers, and County Extension Directors. Of the 68 energy competencies, 36 were rated very important, 28 important, and 4 as having some important to Kansas agriculture. All competencies in category six, energy saving technology for farm vehicles, tractors, machinery and crop production, were rated very important. Category six was the highest-rated category importance to Kansas ariculture, and category eleven other alternate energy sources and storage systems was rated the least important. Because energy technology was important to Kansas agriculture it was recommended educational curricula and materials be developed based on the 68 competencies in this study, and be made available to agriculture instructors at all teaching levels.

  16. Activity of leucine aminopeptidase of Telchin licus licus: an important insect pest of sugarcane.

    PubMed

    Valencia, Jorge W Arboleda; de Sá, Maria Fátima Grossi; Jiménez, Arnubio Valencia

    2014-06-01

    The enzymatic activity of leucine aminopeptidase (EC 3.4.11.1) from the intestinal tract of sugarcane giant borer (Telchin licus licus) was assayed by using a simple and sensitive spectrophotometric assay that uses L-leucyl-2- naphthylamide as substrate. In this assay, L-leucyl-2-naphthylamide is hydrolyzed to produce 2-naphthylamine and Lleucine. The product 2-naphthylamine reacts with Fast Black K and can be monitored using a continuous spectrophotometric measurement at 590 nm. The data on the kinetic parameters indicates that the Km for the L-leucyl-2- naphthylamide at pH 7.0 was found to be lower than those found for other LAP substrates. The Km and Vmax for the LAP were determined to be 84.03 µM and 357.14 enzymatic units mg(-1), respectively. A noticeable difference of LAP activity between the two insect orders tested was observed. This method could be used to screen for natural LAP inhibitors. PMID:24410745

  17. 76 FR 20305 - Consultative Group To Eliminate the Use of Child Labor and Forced Labor in Imported Agricultural...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-12

    ... Foreign Agricultural Service Consultative Group To Eliminate the Use of Child Labor and Forced Labor in Imported Agricultural Products AGENCY: Foreign Agricultural Service, USDA. ACTION: Request for Comment on Guidelines for Eliminating Child and Forced Labor in Agricultural Supply Chains. SUMMARY: Notice is...

  18. Biology and Thermal Death Kinetics of Selected Insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Heat treatments have been suggested as alternatives to fumigation for a variety of postharvest applications. This paper describes the general biology of several economically important insect pests in the international trade of agricultural commodities and presents fundamental thermal death kinetics ...

  19. 7 CFR 51.2122 - Insect injury.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Insect injury. 51.2122 Section 51.2122 Agriculture... Standards for Grades of Shelled Almonds Definitions § 51.2122 Insect injury. Insect injury means that the insect, web, or frass is present or there is definite evidence of insect feeding....

  20. 7 CFR 51.2290 - Insect injury.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Insect injury. 51.2290 Section 51.2290 Agriculture... Standards for Shelled English Walnuts (Juglans Regia) Definitions § 51.2290 Insect injury. Insect injury means that the insect, web, frass or other evidence of insects is present on the portion of kernel....

  1. 7 CFR 51.2008 - Insect injury.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Insect injury. 51.2008 Section 51.2008 Agriculture....2008 Insect injury. Insect injury means that the insect, frass or web is present inside the nut or the kernel shows definite evidence of insect feeding. Metric Conversion Table...

  2. 7 CFR 51.2290 - Insect injury.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Insect injury. 51.2290 Section 51.2290 Agriculture... Standards for Shelled English Walnuts (Juglans Regia) Definitions § 51.2290 Insect injury. Insect injury means that the insect, web, frass or other evidence of insects is present on the portion of kernel....

  3. 7 CFR 51.2290 - Insect injury.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Insect injury. 51.2290 Section 51.2290 Agriculture... Standards for Shelled English Walnuts (Juglans Regia) Definitions § 51.2290 Insect injury. Insect injury means that the insect, web, frass or other evidence of insects is present on the portion of kernel....

  4. 7 CFR 51.2122 - Insect injury.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Insect injury. 51.2122 Section 51.2122 Agriculture... Standards for Grades of Shelled Almonds Definitions § 51.2122 Insect injury. Insect injury means that the insect, web, or frass is present or there is definite evidence of insect feeding....

  5. 7 CFR 51.2008 - Insect injury.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Insect injury. 51.2008 Section 51.2008 Agriculture....2008 Insect injury. Insect injury means that the insect, frass or web is present inside the nut or the kernel shows definite evidence of insect feeding. Metric Conversion Table...

  6. 7 CFR 51.2122 - Insect injury.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Insect injury. 51.2122 Section 51.2122 Agriculture... Standards for Grades of Shelled Almonds Definitions § 51.2122 Insect injury. Insect injury means that the insect, web, or frass is present or there is definite evidence of insect feeding....

  7. The importance of natural habitats to Brazilian free-tailed bats in intensive agricultural landscapes in the Winter Garden Region of Texas, United States

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The conversion of natural lands to agriculture affects the distribution of biological diversity across the landscape. In particular, cropland monocultures alter insect abundance and diversity compared to adjacent natural habitats, but nevertheless can provide large numbers of insect pests as prey i...

  8. Farmers' perception on the importance of variegated grasshopper (Zonocerus variegatus (L.)) in the agricultural production systems of the humid forest zone of Southern Cameroon

    PubMed Central

    Kekeunou, Sévilor; Weise, Stephan; Messi, Jean; Tamò, Manuel

    2006-01-01

    Background Zonocerus variegatus (Linnaeus, 1758) (Orthoptera: Pyrgomorphidae) is known as an agricultural pest in West and Central Africa. However, its importance in the agricultural production system in Cameroon has not been investigated. The study assesses farmers' perception on the importance of Z. variegatus in the agricultural production systems of the humid forest zone of Southern Cameroon. Methods Research was carried out in 5 villages of each of three Agro-Ecological, Cultural and Demographic Blocks (AECD-Blocks) of the Forest Margin Benchmark Area (FMBA). In each village, a semi-structured survey was used; male and female groups of farmers were interviewed separately. Results Z. variegatus is present throughout the humid forest zone of Southern Cameroon, where it is ranked as the third most economically important insect pest of agriculture. In the farmers' opinion, Z. variegatus is a polyphagous insect with little impact on young perennial crops. The length of the pre-farming fallow does not affect Z. variegatus pest pressure in the following crops. The increased impact of the grasshopper observed today in the fields, compared to what existed 10 years ago is as a result of deforestation and increase in surface of herbaceous fallow. The damage caused by Z. variegatus is higher in fields adjacent to C. odorata and herbaceous fallows than in those adjacent to forests and shrubby fallows. The fight against this grasshopper is often done through physical methods carried out by hand, for human consumption. The farmers highlight low usage of the chemical methods and a total absence of biological and ecological methods. Conclusion Farmers' perception have contributed to understanding the status of Z. variegatus in the humid forest zone of Southern Cameroon. The results are in general similar to those obtained in other countries. PMID:16573815

  9. Host races in plant-feeding insects and their importance in sympatric speciation.

    PubMed Central

    Drès, Michele; Mallet, James

    2002-01-01

    The existence of a continuous array of sympatric biotypes - from polymorphisms, through ecological or host races with increasing reproductive isolation, to good species - can provide strong evidence for a continuous route to sympatric speciation via natural selection. Host races in plant-feeding insects, in particular, have often been used as evidence for the probability of sympatric speciation. Here, we provide verifiable criteria to distinguish host races from other biotypes: in brief, host races are genetically differentiated, sympatric populations of parasites that use different hosts and between which there is appreciable gene flow. We recognize host races as kinds of species that regularly exchange genes with other species at a rate of more than ca. 1% per generation, rather than as fundamentally distinct taxa. Host races provide a convenient, although admittedly somewhat arbitrary intermediate stage along the speciation continuum. They are a heuristic device to aid in evaluating the probability of speciation by natural selection, particularly in sympatry. Speciation is thereby envisaged as having two phases: (i) the evolution of host races from within polymorphic, panmictic populations; and (ii) further reduction of gene flow between host races until the diverging populations can become generally accepted as species. We apply this criterion to 21 putative host race systems. Of these, only three are unambiguously classified as host races, but a further eight are strong candidates that merely lack accurate information on rates of hybridization or gene flow. Thus, over one-half of the cases that we review are probably or certainly host races, under our definition. Our review of the data favours the idea of sympatric speciation via host shift for three major reasons: (i) the evolution of assortative mating as a pleiotropic by-product of adaptation to a new host seems likely, even in cases where mating occurs away from the host; (ii) stable genetic differences in

  10. Possible climate warming effects on vegetation, forests, biotic (insect, pathogene) disturbances and agriculture in Central Siberia for 1960- 2050

    NASA Astrophysics Data System (ADS)

    Tchebakova, N. M.; Parfenova, E. I.; Soja, A. J.; Lysanova, G. I.; Baranchikov, Y. N.; Kuzmina, N. A.

    2012-04-01

    Regional Siberian studies have already registered climate warming over the last half a century (1960-2010). Our analysis showed that winters are already 2-3°C warmer in the north and 1-2°C warmer in the south by 2010. Summer temperatures increased by 1°C in the north and by 1-2°C in the south. Change in precipitation is more complicated, increasing on average 10% in middle latitudes and decreasing 10-20% in the south, promoting local drying in already dry landscapes. Our goal was to summarize results of research we have done for the last decade in the context of climate warming and its consequences for biosystems in Central Siberia. We modeled climate change effects on vegetation shifts, on forest composition and agriculture change, on the insect Siberian moth (Dendrolimus suprans sibiricus Tschetv) and pathogene (Lophodermium pinastri Chev) ranges in Central Siberia for a century (1960-2050) based on historical climate data and GCM-predicted data. Principal results are: In the warmer and drier climate projected by these scenarios, Siberian forests are predicted to decrease and shift northwards and forest-steppe and steppe ecosystems are predicted to dominate over 50% of central Siberia due to the dryer climate by 2080. Permafrost is not predicted to thaw deep enough to sustain dark (Pinus sibirica, Abies sibirica, and Picea obovata) taiga. Over eastern Siberia, larch (Larix dahurica) taiga is predicted to continue to be the dominant zonobiome because of its ability to withstand continuous permafrost. The model also predicts new temperate broadleaf forest and forest-steppe habitats; At least half of central Siberia is predicted to be climatically suitable for agriculture at the end of the century although potential croplands would be limited by the availability of suitable soils agriculture in central Siberia would likely benefit from climate warming Crop production may twofold increase as climate warms during the century; traditional crops (grain, potato

  11. New dispenser types for integrated pest management of agriculturally significant insect pests: an algorithm with specialized searching capacity in electronic data bases.

    PubMed

    Hummel, H E; Eisinger, M T; Hein, D F; Breuer, M; Schmid, S; Leithold, G

    2012-01-01

    Pheromone effects discovered some 130 years, but scientifically defined just half a century ago, are a great bonus for basic and applied biology. Specifically, pest management efforts have been advanced in many insect orders, either for purposes or monitoring, mass trapping, or for mating disruption. Finding and applying a new search algorithm, nearly 20,000 entries in the pheromone literature have been counted, a number much higher than originally anticipated. This compilation contains identified and thus synthesizable structures for all major orders of insects. Among them are hundreds of agriculturally significant insect pests whose aggregated damages and costly control measures range in the multibillions of dollars annually. Unfortunately, and despite a lot of effort within the international entomological scene, the number of efficient and cheap engineering solutions for dispensing pheromones under variable field conditions is uncomfortably lagging behind. Some innovative approaches are cited from the relevant literature in an attempt to rectify this situation. Recently, specifically designed electrospun organic nanofibers offer a lot of promise. With their use, the mating communication of vineyard insects like Lobesia botrana (Lep.: Tortricidae) can be disrupted for periods of seven weeks. PMID:23885431

  12. Insect food aiming at Mars emigration

    NASA Astrophysics Data System (ADS)

    Katayama, Naomi; Yamashita, Masamichi; Hashimoto, Hirofumi; Nagasaka, Sanako; Kuwayama, Akemi; Sofue, Megumi

    2012-07-01

    We study insect food aiming at Mars emigration.In space agriculture, insect is the important creature which we cannot miss.It is necessary for the pollination of the plant, and it is rich to protein and lipid as food.I reported that silkworm is an insect necessary for astroponics in particular last time.We make clothes using silk thread, and the pupa becomes the food.In addition, the clothes can make food as protein when we need not to use it. The bee is a very important insect in the space agriculture,too.We examined nutrition of silkworm, bee, grasshopper, snail and the white ant which are necessary for Mars emigration.We will introduce of good balance space foods.We will report many meal menu for Mars emigration.

  13. Estimating the Importance of Private Adaptation to Climate Change in Agriculture: A Review of Empirical Methods

    NASA Astrophysics Data System (ADS)

    Moore, F.; Burke, M.

    2015-12-01

    A wide range of studies using a variety of methods strongly suggest that climate change will have a negative impact on agricultural production in many areas. Farmers though should be able to learn about a changing climate and to adjust what they grow and how they grow it in order to reduce these negative impacts. However, it remains unclear how effective these private (autonomous) adaptations will be, or how quickly they will be adopted. Constraining the uncertainty on this adaptation is important for understanding the impacts of climate change on agriculture. Here we review a number of empirical methods that have been proposed for understanding the rate and effectiveness of private adaptation to climate change. We compare these methods using data on agricultural yields in the United States and western Europe.

  14. Use of shaking treatments and preharvest sprays of pyrethroid insecticides to reduce risk of yellowjackets and other insects on Christmas trees imported into Hawaii.

    PubMed

    Hollingsworth, Robert G; Chastagner, Gary A; Reimer, Neil J; Oishi, Darcy E; Landolt, Peter J; Paull, Robert E

    2009-02-01

    Insects are commonly found by Hawaii's quarantine inspectors on Christmas trees imported from the Pacific Northwest. To reduce the risk of importing yellowjacket (Vespula spp.) queens and other insects, an inspection and tree shaking certification program was begun in 1990. From 1993 to 2006, the annual percentage of shipped containers rated by Hawaii quarantine inspectors as moderately or highly infested with insects was significantly higher for manually shaken trees than for mechanically shaken trees. Between 1993 and 2001, 343 insect species in total were recovered from Christmas trees. Live western yellowjacket [Vespula pensylvanica (Saussure)] queens were intercepted both from containers certified as manually shaken and from containers certified as mechanically shaken. The standard manual shaking protocol removed about one-half of the queens from Douglas fir [Pseudotsuga menziesii (Mirb.) Franco] trees that were naturally infested with western yellowjacket queens. We investigated the use of preharvest sprays of permethrin as a complement to shaking procedures used to control yellowjackets and other insects. Western yellowjacket queens and honey bees (surrogates for wasp pests) were exposed to Noble fir foliage that had been sprayed in the field with permethrin > 6 wk before harvest. Pesticide residues provided complete control (moribundity or mortality) in both species. The sprays did not affect needle retention or quality of Noble fir foliage. We conclude that preharvest sprays of pyrethroid insecticides could be used in combination with mechanical shaking to greatly reduce the quarantine risk of yellowjacket queens and other insects in exported Christmas trees. PMID:19253620

  15. Importance of impacts scenarios for the adaptation of agriculture to climate change

    NASA Astrophysics Data System (ADS)

    Zullo, J.; Macedo, C.; Pinto, H. S.; Assad, E. D.; Koga Vicente, A.

    2012-12-01

    The great possibility that the climate is already changing, and the most drastic way possible, increases the challenge of agricultural engineering, especially in environmentally vulnerable areas and in regions where agriculture has a high economic and social importance. Knowledge of potential impacts that may be caused by changes in water and thermal regimes in coming decades is increasingly strategic, as they allow the development of techniques to adapt agriculture to climate change and therefore minimizes the risk of undesirable impacts, for example, in food and nutritional security. Thus, the main objective of this paper is to describe a way to generate impacts scenarios caused by anomalies of precipitation and temperature in the definition of climate risk zoning of an agricultural crop very important in the tropics, such as the sugar cane, especially in central-southern Brazil, which is one of its main world producers. A key point here is the choice of the climate model to be used, considering that 23 different models were used in the fourth IPCC report published in 2007. The number and range of available models requires the definition of criteria for choosing the most suitable for the preparation of the impacts scenarios. One way proposed and used in this work is based on the definition of two groups of models according to 27 technical attributes of them. The clustering of 23 models in two groups, with a model representing each group (UKMO_HadCM3 and MIROC3.2_medres), assists the generation and comparison of impacts scenarios, making them more representative and useful. Another important aspect in the generation of impacts scenarios is the estimate of the relative importance of the anomalies of precipitation and temperature, which are the most commonly used. To assess the relative importance of the anomalies are generated scenarios considering an anomaly at a time and both together. The impacts scenarios for a high emission of greenhouse gases (A2), from 2010

  16. Recent trends/challenges in irrigated agriculture-Why is irrigation important in a discussion of agricultural migration?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    United States agriculture contributes 16% of the $9 trillion gross domestic product, 8% of U.S. exports, and 17% of employment while providing food to all citizens, despite the fact that only 2% of the U.S. workforces is on farms. Agricultural productivity has grown by 240% since 1948, while agricul...

  17. The importance of an alternative for sustainability of agriculture around the periphery of the Amazon rainforest.

    PubMed

    Moura, Emanoel G; Sena, Virley G L; Corrêa, Mariana S; Aguiar, Alana das C F

    2013-04-01

    The unsustainable use of the soil of the deforested area at the Amazonian border is one of the greatest threats to the rainforest, because it is the predominant cause of shifting cultivation in the region. The sustainable management of soils with low natural fertility is a major challenge for smallholder agriculture in the humid tropics. In the periphery of Brazilian Amazonia, agricultural practices that are recommended for the Brazilian savannah, such as saturating soils with soluble nutrients do not ensure the sustainability of agroecosystems. Improvements in the tilled topsoil cannot be maintained if deterioration of the porous soil structure is not prevented and nutrient losses in the root zone are not curtailed. The information gleaned from experiments affirms that in the management of humid tropical agrosystems, the processes resulting from the interaction between climatic factors and indicators of soil quality must be taken into consideration. It must be remembered that these interactions manifest themselves in ways that cannot be predicted from the paradigm established in the other region like the southeast of Brazil, which is based only on improving the chemical indicators of soil quality. The physical indicators play important role in the sustainable management of the agrosystems of the region and for these reasons must be considered. Therefore, alley cropping is a potential substitute for slash and burn agriculture in the humid tropics with both environmental and agronomic advantages, due to its ability to produce a large amount of residues on the soil surface and its effect on the increase of economic crop productivity in the long term. The article presents some promising patents on the importance of an alternative for sustainability of agriculture. PMID:23305424

  18. 7 CFR 51.2122 - Insect injury.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Insect injury. 51.2122 Section 51.2122 Agriculture..., CERTIFICATION, AND STANDARDS) United States Standards for Grades of Shelled Almonds Definitions § 51.2122 Insect injury. Insect injury means that the insect, web, or frass is present or there is definite evidence...

  19. 7 CFR 51.2122 - Insect injury.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Insect injury. 51.2122 Section 51.2122 Agriculture..., CERTIFICATION, AND STANDARDS) United States Standards for Grades of Shelled Almonds Definitions § 51.2122 Insect injury. Insect injury means that the insect, web, or frass is present or there is definite evidence...

  20. 7 CFR 51.2008 - Insect injury.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Insect injury. 51.2008 Section 51.2008 Agriculture... Standards for Grades of Filberts in the Shell 1 Definitions § 51.2008 Insect injury. Insect injury means that the insect, frass or web is present inside the nut or the kernel shows definite evidence of...

  1. 7 CFR 51.2008 - Insect injury.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Insect injury. 51.2008 Section 51.2008 Agriculture... Standards for Grades of Filberts in the Shell 1 Definitions § 51.2008 Insect injury. Insect injury means that the insect, frass or web is present inside the nut or the kernel shows definite evidence of...

  2. 7 CFR 51.2008 - Insect injury.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Insect injury. 51.2008 Section 51.2008 Agriculture... Standards for Grades of Filberts in the Shell 1 Definitions § 51.2008 Insect injury. Insect injury means that the insect, frass or web is present inside the nut or the kernel shows definite evidence of...

  3. Space Insect-Food Aiming at Mars Emigration

    NASA Astrophysics Data System (ADS)

    Katayama, Naomi; Hashimoto, Hirofumi; Yamashita, Masamichi; Takase, Yoshimi; Kawai, Mika; Space Agriculture Task Force

    We study space insect-food during 10 years. We are aiming at Mars emigration. In space agriculture, insect is the important creature which we cannot miss. It is necessary for the pollination of the plant, and it is rich to protein and lipid as food. We reported that silkworm, bee, grasshopper, snail, fly and termite (white ant) are insects necessary for astroponics in particular last time. We make clothes using silk thread, and the pupa becomes the food. In addition, the clothes can make food as protein when we need not to use it. The bee is a very important insect in the space agriculture, too. We calculated the nourishment ingredient of those insects and thought about ideal space foods which ara necessary for Mars emigration. We will introduce good balance space foods.

  4. Importance of instream wood characteristics for developing restoration designs for channelized agricultural headwater streams

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channelized agricultural headwater streams are a common feature within agricultural watersheds of the Midwestern United States. These small streams have been impacted by the physical and chemical habitat alterations incurred to facilitate agricultural drainage. Quantitative information on the instr...

  5. Identification of functionally important residues in the silkmoth pheromone biosynthesis-activating neuropeptide receptor, an insect ortholog of the vertebrate Neuromedin U Receptor

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The biosynthesis of sex pheromone components in many lepidopteran insects is regulated by interactions between pheromone biosynthesis-activating neuropeptide (PBAN) and the PBAN receptor (PBANR), a class-A G-protein-coupled receptor (GPCR). To identify functionally important amino acid residues in t...

  6. Supplementary calcium ameliorates ammonium toxicity by improving water status in agriculturally important species

    PubMed Central

    Hernández-Gómez, Elvia; Valdez-Aguilar, Luis A.; Cartmill, Donita L.; Cartmill, Andrew D.; Alia-Tajacal, Irán

    2015-01-01

    Fertilization of agricultural plants with ammonium (NH4+) is often desirable because it is less susceptible to leaching than nitrate (NO3−), reducing environmental pollution, risk to human health and economic loss. However, a number of important agricultural species exhibit a reduction in growth when fertilized with NH4+, and increasing the tolerance to NH4+ may be of importance for the establishment of sustainable agricultural systems. The present study explored the feasibility of using calcium (Ca) to increase the tolerance of bell pepper (Capsicum annuum) to NH4+ fertilization. Although NH4+ at proportions ≥25 % of total nitrogen (N) decreased leaf dry mass (DM), supplementary Ca ameliorated this decrease. Increasing NH4+ resulted in decreased root hydraulic conductance (Lo) and root water content (RWC), suggesting that water uptake by roots was impaired. The NH4+-induced reductions in Lo and RWC were mitigated by supplementary Ca. Ammonium induced increased damage to the cell membranes through lipid peroxidation, causing increased electrolyte leakage; Ca did not reduce lipid peroxidation and resulted in increased electrolyte leakage, suggesting that the beneficial effects of Ca on the tolerance to NH4+ may be more of a reflection on its effect on the water status of the plant. Bell pepper plants that received NO3−N had a low concentration of NH4+ in the roots but a high concentration in the leaves, probably due to the high nitrate reductase activity observed. Ammonium nutrition depressed the uptake of potassium, Ca and magnesium, while increasing that of phosphorus. The results obtained in the present study indicate that NH4+ caused growth reduction, nutrient imbalance, membrane integrity impairment, increased activity of antioxidant enzymes and affected water relations. Supplementary Ca partially restored growth of leaves by improving root Lo and water relations, and our results suggest that it may be used as a tool to increase the tolerance to NH4

  7. Supplementary calcium ameliorates ammonium toxicity by improving water status in agriculturally important species.

    PubMed

    Hernández-Gómez, Elvia; Valdez-Aguilar, Luis A; Cartmill, Donita L; Cartmill, Andrew D; Alia-Tajacal, Irán

    2015-01-01

    Fertilization of agricultural plants with ammonium [Formula: see text] is often desirable because it is less susceptible to leaching than nitrate [Formula: see text] reducing environmental pollution, risk to human health and economic loss. However, a number of important agricultural species exhibit a reduction in growth when fertilized with [Formula: see text] and increasing the tolerance to [Formula: see text] may be of importance for the establishment of sustainable agricultural systems. The present study explored the feasibility of using calcium (Ca) to increase the tolerance of bell pepper (Capsicum annuum) to [Formula: see text] fertilization. Although [Formula: see text] at proportions ≥25 % of total nitrogen (N) decreased leaf dry mass (DM), supplementary Ca ameliorated this decrease. Increasing [Formula: see text] resulted in decreased root hydraulic conductance (Lo) and root water content (RWC), suggesting that water uptake by roots was impaired. The [Formula: see text]-induced reductions in Lo and RWC were mitigated by supplementary Ca. Ammonium induced increased damage to the cell membranes through lipid peroxidation, causing increased electrolyte leakage; Ca did not reduce lipid peroxidation and resulted in increased electrolyte leakage, suggesting that the beneficial effects of Ca on the tolerance to [Formula: see text] may be more of a reflection on its effect on the water status of the plant. Bell pepper plants that received [Formula: see text] had a low concentration of [Formula: see text] in the roots but a high concentration in the leaves, probably due to the high nitrate reductase activity observed. Ammonium nutrition depressed the uptake of potassium, Ca and magnesium, while increasing that of phosphorus. The results obtained in the present study indicate that [Formula: see text] caused growth reduction, nutrient imbalance, membrane integrity impairment, increased activity of antioxidant enzymes and affected water relations. Supplementary Ca

  8. Insect Phylogenomics

    PubMed Central

    Behura, Susanta K.

    2015-01-01

    With the advent of next-generation sequencing methods, phylogenetics has taken a new turn in the recent years. Phylogenomics, the integration of phylogenetics with genome data, has emerged as a powerful approach to study systematics and evolution of species. Recently, breakthrough researches employing phylogenomic tools have provided better insights into the timing and pattern of insect evolution. The next-generation sequencing methods are now increasingly used by entomologists to generate genomic and transcript sequences of various insect species and strains. These data provide opportunities for comparative genomics and large-scale multigene phylogenies of diverse lineages of insects. Phylogenomic investigations help us better understand systematic and evolutionary relationships of insect species that play important roles as herbivores, predators, detritivores, pollinators, or disease vectors. It is important that we critically assess the prospects and limitations of phylogenomic methods. In this review, I describe the current status, outline the major challenges, and remark on potential future applications of phylogenomic tools in studying insect systematics and evolution. PMID:25963452

  9. Agriculture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Agriculture within the United States is varied and produces a large value ($200 billion in 2002) of production across a wide range of plant and animal production systems. Because of this diversity, changes in climate will likely impact agriculture throughout the United States. Climate affects crop, ...

  10. Potential applications of insect symbionts in biotechnology.

    PubMed

    Berasategui, Aileen; Shukla, Shantanu; Salem, Hassan; Kaltenpoth, Martin

    2016-02-01

    Symbiotic interactions between insects and microorganisms are widespread in nature and are often the source of ecological innovations. In addition to supplementing their host with essential nutrients, microbial symbionts can produce enzymes that help degrade their food source as well as small molecules that defend against pathogens, parasites, and predators. As such, the study of insect ecology and symbiosis represents an important source of chemical compounds and enzymes with potential biotechnological value. In addition, the knowledge on insect symbiosis can provide novel avenues for the control of agricultural pest insects and vectors of human diseases, through targeted manipulation of the symbionts or the host-symbiont associations. Here, we discuss different insect-microbe interactions that can be exploited for insect pest and human disease control, as well as in human medicine and industrial processes. Our aim is to raise awareness that insect symbionts can be interesting sources of biotechnological applications and that knowledge on insect ecology can guide targeted efforts to discover microorganisms of applied value. PMID:26659224

  11. Mathematical modelling of antimicrobial resistance in agricultural waste highlights importance of gene transfer rate.

    PubMed

    Baker, Michelle; Hobman, Jon L; Dodd, Christine E R; Ramsden, Stephen J; Stekel, Dov J

    2016-04-01

    Antimicrobial resistance is of global concern. Most antimicrobial use is in agriculture; manures and slurry are especially important because they contain a mix of bacteria, including potential pathogens, antimicrobial resistance genes and antimicrobials. In many countries, manures and slurry are stored, especially over winter, before spreading onto fields as organic fertilizer. Thus, these are a potential location for gene exchange and selection for resistance. We develop and analyse a mathematical model to quantify the spread of antimicrobial resistance in stored agricultural waste. We use parameters from a slurry tank on a UK dairy farm as an exemplar. We show that the spread of resistance depends in a subtle way on the rates of gene transfer and antibiotic inflow. If the gene transfer rate is high, then its reduction controls resistance, while cutting antibiotic inflow has little impact. If the gene transfer rate is low, then reducing antibiotic inflow controls resistance. Reducing length of storage can also control spread of resistance. Bacterial growth rate, fitness costs of carrying antimicrobial resistance and proportion of resistant bacteria in animal faeces have little impact on spread of resistance. Therefore, effective treatment strategies depend critically on knowledge of gene transfer rates. PMID:26906100

  12. Plant growth promotion in cereal and leguminous agricultural important plants: from microorganism capacities to crop production.

    PubMed

    Pérez-Montaño, F; Alías-Villegas, C; Bellogín, R A; del Cerro, P; Espuny, M R; Jiménez-Guerrero, I; López-Baena, F J; Ollero, F J; Cubo, T

    2014-01-01

    Plant growth-promoting rhizobacteria (PGPR) are free-living bacteria which actively colonize plant roots, exerting beneficial effects on plant development. The PGPR may (i) promote the plant growth either by using their own metabolism (solubilizing phosphates, producing hormones or fixing nitrogen) or directly affecting the plant metabolism (increasing the uptake of water and minerals), enhancing root development, increasing the enzymatic activity of the plant or "helping" other beneficial microorganisms to enhance their action on the plants; (ii) or may promote the plant growth by suppressing plant pathogens. These abilities are of great agriculture importance in terms of improving soil fertility and crop yield, thus reducing the negative impact of chemical fertilizers on the environment. The progress in the last decade in using PGPR in a variety of plants (maize, rice, wheat, soybean and bean) along with their mechanism of action are summarized and discussed here. PMID:24144612

  13. The relative importance of microbial nitrate reduction processes in an agriculturally-impacted estuary

    NASA Astrophysics Data System (ADS)

    Cardarelli, E.; Francis, C. A.

    2013-12-01

    Human activities are increasing reactive nitrogen levels worldwide. Reactive nitrogen exists largely as nitrate and may be ecologically harmful to nutrient-limited systems. Nitrate loadings to the environment may be transformed by the microbial nitrate reduction processes of denitrification (converting nitrate to dinitrogen gas), or of dissimilatory nitrate reduction to ammonium (DNRA) (allowing reactive nitrogen to persist). The predominant nitrate reduction pathway largely determines the nitrogen removal capacity of the estuary. Therefore, identifying the relative importance of denitrification and DNRA in a given system provides insight into how much nitrate is transformed to dinitrogen and ammonium. Estuary sediments often have high nitrate reduction rates, but the environmental factors that determine which process prevails are underexplored. Nitrate availability and salinity are thought to influence which nitrate reduction process predominates. Elkhorn Slough is a small California estuary that experiences a range of nitrate concentrations (0 to over 2,000 μM) and salinities (0 to 33.5) depending on the agricultural runoff introduced through the Old Salinas River and the tidal influence. This study investigates how the fluctuating nutrient and salinity conditions found over the diel cycle at the interface of the Old Salinas River and Elkhorn Slough influences the nitrogen transformation rates observed. Benthic denitrification and DNRA are evaluated using whole sediment core incubations amended with an overlying 15NO3- labeled pool. Rates of denitrification and DNRA in the sediment are calculated using the isotope pairing technique. The results of this research will help elucidate the relative importance of dissimilatory nitrate removal pathways in an agriculturally-impacted estuary and ultimately reveal whether anthropogenic nitrate inputs are preserved or removed from the system.

  14. Competition among agricultural pest insects and its role in pest outbreaks associated with trasgenic Bt-cotton

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Empirical studies on the ecological causes of agricultural pest outbreaks have focused primarily on two biotic factors—release from natural enemies and changes in host plant quality. Release from competition, on the other hand, has been theorized as a potential cause but never tested. With the exp...

  15. Agricultural waste from the tequila industry as substrate for the production of commercially important enzymes.

    PubMed

    Huitron, C; Perez, R; Sanchez, A E; Lappe, P; Rocha Zavaleta, L

    2008-01-01

    Approximately 1 million tons of Agave tequilana plants are processed annually by the Mexican Tequila industry generating vast amounts of agricultural waste. The aim of this study was to investigate the potential use of Agave tequilana waste as substrate for the production of commercially important enzymes. Two strains of Aspergillus niger (CH-A-2010 and CH-A-2016), isolated from agave fields, were found to grow and propagate in submerged cultures using Agave tequilana waste as substrate. Isolates showed simultaneous extracellular inulinase, xylanase, pectinase, and cellulase activities. Aspergillus CH-A-2010 showed the highest production of inulinase activity (1.48 U/ml), whereas Aspergillus niger CH-A-2016 produced the highest xylanase (1.52 U/ml) and endo-pectinase (2.7U/ml) activities. In both cases production of enzyme activities was significantly higher on Agave tequilana waste than that observed on lemon peel and specific polymeric carbohydrates. Enzymatic hydrolysis of raw A. tequilana stems and leaves, by enzymes secreted by the isolates yielded maximum concentrations of reducing sugars of 28.2 g/l, and 9.9 g/l respectively. In conclusion, Agave tequilana waste can be utilized as substrate for the production of important biotechnological enzymes. PMID:18833660

  16. How Do Insects Help the Environment?

    ERIC Educational Resources Information Center

    Hevel, Gary

    2005-01-01

    There are some 5 to 30 million insect species estimated in the world--and the majority of these have yet to be collected or named by science! Of course, the most well known insects are those that cause disease or compete for human agricultural products, but these insects represent only a small fraction of the world's insect population. In reality,…

  17. Importance of wetland landscape structure to shorebirds wintering in an agricultural valley

    USGS Publications Warehouse

    Taft, Oriane W.; Haig, Susan M.

    2006-01-01

    Only recently has the influence of landscape structure on habitat use been a research focus in wetland systems. During non-breeding periods when food can be locally limited, wetland spatial pattern across a landscape may be of great importance in determining wetland use. We studied the influence of landscape structure on abundances of wintering Dunlin (Calidris alpina) and Killdeer (Charadrius vociferus) observed on wetlands in the agricultural Willamette Valley of Oregon, USA, during two winters (1999a??2000, 2000a??2001) of differing rainfall. We examined (1) shorebird use within a sample of 100 km2 regions differing in landscape structure (hectares of shorebird habitat [wet, unvegetated]) and (2) use of sites differing in landscape context (area of shorebird habitat within a species-defined radius). For use of sites, we also assessed the influence of two local characteristics: percent of soil exposed and area of wet habitat. We analyzed data using linear regression and information-theoretic modeling. During the dry winter (2000a??2001), Dunlin were attracted to regions with more wetland habitat and their abundances at sites increased with greater area of shorebird habitat within both the site and the surrounding landscape. In contrast, Dunlin abundances at sites were related to availability of habitat at only a local scale during the wet winter (1999a??2000). Regional habitat availability was of little importance in predicting Killdeer distributions, and Killdeer site use appeared unrelated to habitat distributions at both landscape and local scales. Results suggest prioritizing sites for conservation that are located in areas with high wetland coverage.

  18. The importance of instream habitat modifications for restoring channelized agricultural headwater streams

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Science based information on the influence of restoration practices on fishes within channelized agricultural headwater streams in the Midwestern United States is currently lacking. Understanding fish-habitat relationships and fish responses to specific restoration practices will provide informatio...

  19. In-flight disinsection as an efficacious procedure for preventing international transport of insects of public health importance.

    PubMed Central

    Russell, R. C.; Paton, R.

    1989-01-01

    Aircraft disinsection with aerosol insecticides during flight has generally been held to be inadvisable because it was assumed that the insecticides would be rapidly removed by the cabin air-conditioning system. We have developed protocols to deliver 2% d-phenothrin at a dose of 35 g per 100 m3 in various aircraft, and trials undertaken on Boeing 747 and 767 aircraft showed that their air-conditioning systems do not preclude effective disinsection. Mortality levels of 100% for Culex quinquefasciatus and Musca domestica test insects were recorded under normal operating conditions during routine scheduled passenger flights with disinsection procedures undertaken at "blocks-away" or at "top-of-descent". As a result, "top-of-descent" disinsection has been introduced as the recommended procedure for aircraft landing in Australia. PMID:2611975

  20. Insects, Insecticides and Hormesis: Evidence and Considerations for Study

    PubMed Central

    Cutler, G. Christopher

    2013-01-01

    Insects are ubiquitous, crucial components of almost all terrestrial and fresh water ecosystems. In agricultural settings they are subjected to, intentionally or unintentionally, an array of synthetic pesticides and other chemical stressors. These ecological underpinnings, the amenability of insects to laboratory and field experiments, and our strong knowledgebase in insecticide toxicology, make the insect-insecticide model an excellent one to study many questions surrounding hormesis. Moreover, there is practical importance for agriculture with evidence of pest population growth being accelerated by insecticide hormesis. Nevertheless, insects have been underutilized in studies of hormesis. Where hormesis hypotheses have been tested, results clearly demonstrate stimulatory effects on multiple taxa as measured through several biological endpoints, both at individual and population levels. However, many basic questions are outstanding given the myriad of chemicals, responses, and ecological interactions that are likely to occur. PMID:23930099

  1. Insect Ferritins: typical or atypical?

    PubMed Central

    Pham, Daphne Q. D.; Winzerling, Joy J.

    2010-01-01

    Insects transmit millions of cases of disease each year, and cost millions of dollars in agricultural losses. The control of insect-borne diseases is vital for numerous developing countries, and the management of agricultural insect pests is a very serious business for developed countries. Control methods should target insect-specific traits in order to avoid non-target effects, especially in mammals. Since insect cells have had a billion years of evolutionary divergence from those of vertebrates, they differ in many ways that might be promising for the insect control field—especially, in iron metabolism because current studies have indicated that significant differences exist between insect and mammalian systems. Insect iron metabolism differs from that of vertebrates in the following respects. Insect ferritins have a heavier mass than mammalian ferritins. Unlike their mammalian counterparts, the insect ferritin subunits are often glycosylated and are synthesized with a signal peptide. The crystal structure of insect ferritin also shows a tetrahedral symmetry consisting of 12 heavy chain and 12 light chain subunits in contrast to that of mammalian ferritin that exhibits an octahedral symmetry made of 24 heavy chain and 24 light chain subunits. Insect ferritins associate primarily with the vacuolar system and serve as iron transporters—quite the opposite of the mammalian ferritins, which are mainly cytoplasmic and serve as iron storage proteins. This review will discuss these differences. PMID:20230873

  2. Entomophagy and space agriculture

    NASA Astrophysics Data System (ADS)

    Katayama, N.; Ishikawa, Y.; Takaoki, M.; Yamashita, M.; Nakayama, S.; Kiguchi, K.; Kok, R.; Wada, H.; Mitsuhashi, J.; Space Agriculture Task Force, J.

    Supplying food for human occupants remains one of the primary issues in engineering space habitation Evidently for long-term occupation on a distant planet it is necessary to start agriculture on site Historically humans have consumed a variety of animals and it is required to fill our nutritional need when they live in space Among many candidate group and species of animal to breed in space agriculture insects are of great interest since they have a number of advantages over mammals and other vertebrates or invertebrates About 70-75 of animal species is insects and they play an important role in materials recycle loop of terrestrial biosphere at their various niche For space agriculture we propose several insect species such as the silkworm Bombyx mori the drugstore beetle Stegobium paniceum and the termite Macrotermes subhyalinus Among many advantages these insects do not compete with human in terms of food resources but convert inedible biomass or waste into an edible food source for human The silkworm has been domesticated since 5 000 years ago in China Silk moth has lost capability of flying after its domestication history This feature is advantageous in control of their breeding Silkworm larvae eat specifically mulberry leaves and metamorphose in their cocoon Silk fiber obtained from cocoon can be used to manufacture textile Farming system of the drugstore beetle has been well established Both the drugstore beetle and the termite are capable to convert cellulose or other inedible biomass

  3. The first complete mitochondrial genome of a Belostomatidae species, Lethocerus indicus, the giant water bug: An important edible insect.

    PubMed

    Devi, Kshetrimayum Miranda; Shantibala, Tourangbam; Debaraj, Hajarimayum

    2016-10-10

    Lethocerus indicus of the family Belostomatidae is one of the most preferred and delicious edible insects in different parts of South-East Asia including North-East, India. The mitogenome of L. indicus represents the first complete mitogenome sequence of a Belostomatidae species in Heteroptera order. The mitogenome of L. indicus is 16,251bp and contains 37 genes including 13 protein coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a large non-coding region. The genome has a typical gene order which is identical to other Heteroptera species. All tRNAs exhibit the classic cloverleaf secondary structure except tRNASer (AGN). All the PCGs employ a complete translation termination codon either TAA or TAG except COII. The nucleotide composition showed heavy biased toward AT accounting to 70.9% of total mitogenome. The overall A+T content of L. indicus mitogenome was comparatively lower than some other Heteropteran bugs mitogenomes. The control region is divided into seven different parts which includes the putative stem loop, repeats, tandem repeats, GC and AT rich regions. The phylogenetic relationship based on maximum-likelihood method using all protein coding genes was congruent with the traditional morphological classification that Belostomatidae is closely related to Nepidae. The complete mitogenome sequence of L. indicus provides fundamental data useful in conservation genetics and aquaculture diversification. PMID:27390089

  4. 7 CFR 319.8-20 - Importations by the Department of Agriculture.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... such conditions as may be prescribed by the Deputy Administrator of the Plant Protection and Quarantine Programs, which conditions may include clearance through the New Crops Research Branch of the Plant Science... PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Foreign Cotton...

  5. 7 CFR 319.8-20 - Importations by the Department of Agriculture.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... such conditions as may be prescribed by the Deputy Administrator of the Plant Protection and Quarantine Programs, which conditions may include clearance through the New Crops Research Branch of the Plant Science... PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Foreign Cotton...

  6. The Importance of Motivational Appeals to Cooperative Extension Agricultural Clientele. Summary of Research.

    ERIC Educational Resources Information Center

    Wilson, Gary; Newcomb, L. H.

    A study was conducted to determine the relationship of certain motivational appeals to the extent of participation of extension clientele, as perceived by these clientele. A stratified random sample of thirty counties from the ten extension supervisory areas of Ohio was used for the study. This sample provided for 395 adult agricultural clientele…

  7. Agricultural By-Products Turned into Important Materials with Adsorptive Properties

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This presentation will summarize the use of agricultural by-products (e.g., animal manure and plant waste) as starting materials to adsorb environmental contaminants such as mercury from air, ammonia from air, metal ions from water, and chlorinated organics from water. The results show that the mat...

  8. Assessing the importance of agricultural management practices to reduce the ecological risk of pesticides

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The use of pesticides in agriculture, their potential to be transported beyond the intended target, and their possible risk to human and environmental health has been of public concern for many years. We utilized 5 years of field data from 3 vegetable production systems to evaluate the ability of ag...

  9. 75 FR 11512 - Consultative Group to Eliminate the Use of Child Labor and Forced Labor in Imported Agricultural...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-11

    ... Foreign Agricultural Service Consultative Group to Eliminate the Use of Child Labor and Forced Labor in... public meeting of the Consultative Group to Eliminate the Use of Child Labor and Forced Labor in Imported... input from the public regarding the Consultative Group's statutory mandate to develop...

  10. Identification, functional characterization, and pharmacological profile of a serotonin type-2b receptor in the medically important insect, Rhodnius prolixus

    PubMed Central

    Paluzzi, Jean-Paul V.; Bhatt, Garima; Wang, Chang-Hui J.; Zandawala, Meet; Lange, Angela B.; Orchard, Ian

    2015-01-01

    In the Chagas disease vector, Rhodnius prolixus, two diuretic hormones act synergistically to dramatically increase fluid secretion by the Malpighian tubules (MTs) during the rapid diuresis that is initiated upon engorgement of vertebrate blood. One of these diuretic hormones is the biogenic amine, serotonin (5-hydroxytryptamine, 5-HT), which controls a variety of additional activities including cuticle plasticization, salivary gland secretion, anterior midgut absorption, cardioacceleratory activity, and myotropic activities on a number of visceral tissues. To better understand the regulatory mechanisms linked to these various physiological actions of serotonin, we have isolated and characterized a serotonin type 2b receptor in R. prolixus, Rhopr5HTR2b, which shares sequence similarity to the vertebrate serotonin type 2 receptors. Rhopr5HTR2b transcript is enriched in well-recognized physiological targets of serotonin, including the MTs, salivary glands and dorsal vessel (i.e., insect heart). Notably, Rhopr5HTR2b was not enriched in the anterior midgut where serotonin stimulates absorption and elicits myotropic control. Using a heterologous functional receptor assay, we examined Rhopr5HTR2b activation characteristics and its sensitivity to potential agonists, antagonists, and other biogenic amines. Rhopr5HTR2b is dose-dependently activated by serotonin with an EC50 in the nanomolar range. Rhopr5HTR2b is sensitive to alpha-methyl serotonin and is inhibited by a variety of serotonin receptor antagonists, including propranolol, spiperone, ketanserin, mianserin, and cyproheptadine. In contrast, the cardioacceleratory activity of serotonin revealed a unique pharmacological profile, with no significant response induced by alpha-methyl serotonin and insensitivity to ketanserin and mianserin. This distinct agonist/antagonist profile indicates that a separate serotonin receptor type may mediate cardiomodulatory effects controlled by serotonin in R. prolixus. PMID:26041983

  11. Identification, functional characterization, and pharmacological profile of a serotonin type-2b receptor in the medically important insect, Rhodnius prolixus.

    PubMed

    Paluzzi, Jean-Paul V; Bhatt, Garima; Wang, Chang-Hui J; Zandawala, Meet; Lange, Angela B; Orchard, Ian

    2015-01-01

    In the Chagas disease vector, Rhodnius prolixus, two diuretic hormones act synergistically to dramatically increase fluid secretion by the Malpighian tubules (MTs) during the rapid diuresis that is initiated upon engorgement of vertebrate blood. One of these diuretic hormones is the biogenic amine, serotonin (5-hydroxytryptamine, 5-HT), which controls a variety of additional activities including cuticle plasticization, salivary gland secretion, anterior midgut absorption, cardioacceleratory activity, and myotropic activities on a number of visceral tissues. To better understand the regulatory mechanisms linked to these various physiological actions of serotonin, we have isolated and characterized a serotonin type 2b receptor in R. prolixus, Rhopr5HTR2b, which shares sequence similarity to the vertebrate serotonin type 2 receptors. Rhopr5HTR2b transcript is enriched in well-recognized physiological targets of serotonin, including the MTs, salivary glands and dorsal vessel (i.e., insect heart). Notably, Rhopr5HTR2b was not enriched in the anterior midgut where serotonin stimulates absorption and elicits myotropic control. Using a heterologous functional receptor assay, we examined Rhopr5HTR2b activation characteristics and its sensitivity to potential agonists, antagonists, and other biogenic amines. Rhopr5HTR2b is dose-dependently activated by serotonin with an EC50 in the nanomolar range. Rhopr5HTR2b is sensitive to alpha-methyl serotonin and is inhibited by a variety of serotonin receptor antagonists, including propranolol, spiperone, ketanserin, mianserin, and cyproheptadine. In contrast, the cardioacceleratory activity of serotonin revealed a unique pharmacological profile, with no significant response induced by alpha-methyl serotonin and insensitivity to ketanserin and mianserin. This distinct agonist/antagonist profile indicates that a separate serotonin receptor type may mediate cardiomodulatory effects controlled by serotonin in R. prolixus. PMID:26041983

  12. The importance of animal cognition in agricultural animal production systems: an overview.

    PubMed

    Curtis, S E; Stricklin, W R

    1991-12-01

    To describe and then fulfill agricultural animals' needs, we must learn more about their fundamental psychological and behavioral processes. How does this animal feel? Is that animal suffering? Will we ever be able to know these things? Scientists specializing in animal cognition say that there are numerous problems but that they can be overcome. Recognition by scientists of the notion of animal awareness has been increasing in recent years, because of the work of Griffin and others. Feeling, thinking, remembering, and imagining are cognitive processes that are factors in the economic and humane production of agricultural animals. It has been observed that the animal welfare debate depends on two controversial questions: Do animals have subjective feelings? If they do, can we find indicators that reveal them? Here, indirect behavioral analysis approaches must be taken. Moreover, the linear additivity of several stressor effects on a variety of animal traits suggests that some single phenomenon is acting as a "clearinghouse" for many or all of the stresses acting on an animal at any given time, and this phenomenon might be psychological stress. Specific situations animals may encounter in agricultural production settings are discussed with respect to the animals' subjective feelings. PMID:1808193

  13. "If it looks like a duck…" - why humans need to focus on different approaches than insects if we are to become efficiently and effectively ultrasocial.

    PubMed

    Aitken, Kenneth John

    2016-01-01

    The parallels between the agricultural successes of ultrasocial insects and those of humans are interesting and potentially important. There are a number of important caveats, however, including the relative complexities of insect reproduction, their more rigidly determined altricial patterns of social behaviour, the roles of post-reproductive group members, and differences in the known factors involved in ultrasocietal collapse. PMID:27561700

  14. Importance of non CO2 fluxes for agricultural ecosystems - understanding the mechanisms and drivers

    NASA Astrophysics Data System (ADS)

    Klumpp, Katja

    2014-05-01

    In agriculture, a large proportion (about 89%) of greenhouse gas (GHG) emission saving potential may be achieved by means of soil C sequestration. Not surprising that exchange of carbon dioxide (CO2) has been a main research objective during last decades. In spite of this, in agricultural ecosytems (i.e. grassland and croplands) a large proportion of total emissions (about 18% in CO2e worldwide) are linked to non CO2 fluxes (about 50% N2O, 40% CH4 in contraste to 10%CO2). Those emissions are however, diffuse, for example N2O, is emitted on almost all cultivated land, and all humid grasslands emit CH4 related to watertable. However, those emissions can vary largely from one site to another or from one farming system to another, while some studies even report a fixation of CH4 and N2O by grass- and croplands, not to mention the impacts of climate change on fluxes. Finally, given the large number of findings, along with their significant diversity, complicates both estimation of these emissions and the mechanism that the public authorities could implement to encourage their reduction. To determine effective mitigation options, a better knowledge on the drivers of CH4/N2O as well as their temporal and spatial variability are of particular interest. At present, more information is needed on i) the impact of agricultural practices and the contribution of CH4 and N2O to the GHG budgets within contrasting systems, ii) differences among climate regions and climate impacts, and iii) impact of managing soil microbial functioning (through plant diverstiy, litter inputs, etc). This presentation will review recent studies to highlight some new findings on the mentioned topics.

  15. Identification of functionally important residues of the silkmoth pheromone biosynthesis-activating neuropeptide receptor, an insect ortholog of the vertebrate neuromedin U receptor.

    PubMed

    Kawai, Takeshi; Katayama, Yukie; Guo, Linjun; Liu, Desheng; Suzuki, Tatsuya; Hayakawa, Kou; Lee, Jae Min; Nagamine, Toshihiro; Hull, J Joe; Matsumoto, Shogo; Nagasawa, Hiromichi; Tanokura, Masaru; Nagata, Koji

    2014-07-01

    The biosynthesis of sex pheromone components in many lepidopteran insects is regulated by the interaction between pheromone biosynthesis-activating neuropeptide (PBAN) and the PBAN receptor (PBANR), a class A G-protein-coupled receptor. To identify functionally important amino acid residues in the silkmoth PBANR, a series of 27 alanine substitutions was generated using a PBANR chimera C-terminally fused with enhanced GFP. The PBANR mutants were expressed in Sf9 insect cells, and their ability to bind and be activated by a core PBAN fragment (C10PBAN(R2K)) was monitored. Among the 27 mutants, 23 localized to the cell surface of transfected Sf9 cells, whereas the other four remained intracellular. Reduced binding relative to wild type was observed with 17 mutants, and decreased Ca(2+) mobilization responses were observed with 12 mutants. Ala substitution of Glu-95, Glu-120, Asn-124, Val-195, Phe-276, Trp-280, Phe-283, Arg-287, Tyr-307, Thr-311, and Phe-319 affected both binding and Ca(2+) mobilization. The most pronounced effects were observed with the E120A mutation. A molecular model of PBANR indicated that the functionally important PBANR residues map to the 2nd, 3rd, 6th, and 7th transmembrane helices, implying that the same general region of class A G-protein-coupled receptors recognizes both peptidic and nonpeptidic ligands. Docking simulations suggest similar ligand-receptor recognition interactions for PBAN-PBANR and the orthologous vertebrate pair, neuromedin U (NMU) and NMU receptor (NMUR). The simulations highlight the importance of two glutamate residues, Glu-95 and Glu-120, in silkmoth PBANR and Glu-117 and Glu-142 in human NMUR1, in the recognition of the most functionally critical region of the ligands, the C-terminal residue and amide. PMID:24847080

  16. Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor*

    PubMed Central

    Kawai, Takeshi; Katayama, Yukie; Guo, Linjun; Liu, Desheng; Suzuki, Tatsuya; Hayakawa, Kou; Lee, Jae Min; Nagamine, Toshihiro; Hull, J. Joe; Matsumoto, Shogo; Nagasawa, Hiromichi; Tanokura, Masaru; Nagata, Koji

    2014-01-01

    The biosynthesis of sex pheromone components in many lepidopteran insects is regulated by the interaction between pheromone biosynthesis-activating neuropeptide (PBAN) and the PBAN receptor (PBANR), a class A G-protein-coupled receptor. To identify functionally important amino acid residues in the silkmoth PBANR, a series of 27 alanine substitutions was generated using a PBANR chimera C-terminally fused with enhanced GFP. The PBANR mutants were expressed in Sf9 insect cells, and their ability to bind and be activated by a core PBAN fragment (C10PBANR2K) was monitored. Among the 27 mutants, 23 localized to the cell surface of transfected Sf9 cells, whereas the other four remained intracellular. Reduced binding relative to wild type was observed with 17 mutants, and decreased Ca2+ mobilization responses were observed with 12 mutants. Ala substitution of Glu-95, Glu-120, Asn-124, Val-195, Phe-276, Trp-280, Phe-283, Arg-287, Tyr-307, Thr-311, and Phe-319 affected both binding and Ca2+ mobilization. The most pronounced effects were observed with the E120A mutation. A molecular model of PBANR indicated that the functionally important PBANR residues map to the 2nd, 3rd, 6th, and 7th transmembrane helices, implying that the same general region of class A G-protein-coupled receptors recognizes both peptidic and nonpeptidic ligands. Docking simulations suggest similar ligand-receptor recognition interactions for PBAN-PBANR and the orthologous vertebrate pair, neuromedin U (NMU) and NMU receptor (NMUR). The simulations highlight the importance of two glutamate residues, Glu-95 and Glu-120, in silkmoth PBANR and Glu-117 and Glu-142 in human NMUR1, in the recognition of the most functionally critical region of the ligands, the C-terminal residue and amide. PMID:24847080

  17. Sterile Insect Quality

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This chapter discusses the history of the development of quality control tchnology, the principles and philosophy of assessing insect quality, and the relative importance of the various parameters used to assess insect quality in the context of mass-rearing for the SIT. Quality control is most devel...

  18. The Potential of Pigeonpea (Cajanus cajan) for Producing Important Components of Renewable Energy and Agricultural Products

    NASA Astrophysics Data System (ADS)

    Gwata, E.

    2012-04-01

    In agricultural systems, sustainable crop production is critical in meeting both environmental requirements and the limitations of drought imposed by the effects of global warming. The inputs for crop production and end use of the products should determine the choice of a crop particularly in environments prone to droughts. The objective of this paper is to highlight why a multi-purpose grain legume such as pigeonpea is an ideal crop that can be utilized for producing renewable energy. Firstly, it is highly tolerant to drought and does not require additional soil moisture after the seedling growth stage. The deep tape root extracts moisture and nutrients from deep layers of the soil concomitantly allowing for efficient nutrient recycling. The piscidic acid which is exuded from the roots enhances the solubilization of phosphorus in order to make it available for plant uptake. Secondly, the grain of pigeonpea is suitable for both human food and feedstocks. The grain is rich in oil, vitamins, minerals and protein. The grain can also be used for producing biofuel. In many countries particularly in the developing world, the stover is used as fuel wood or building (roofing) material, thus alleviating pressure on forest products. The crop is grown without the application of inorganic fertilizers as it can fix atmospheric nitrogen symbiotically in its root nodules. Pigeonpea is also ratoonable, producing two or more harvests per season. In addition, it is grown in mixed cropping systems thus optimizing land use. In these regards, pigeonpea is sustainable and environmentally friendly choice for agricultural production of food and energy balance.

  19. Irradiating insect pests

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This is a non-technical article focusing on phytosanitary uses of irradiation. In a series of interview questions, I present information on the scope of the invasive species problem and the contribution of international trade in agricultural products to the movement of invasive insects. This is foll...

  20. Mechanisms by which pesticides affect insect immunity

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The known effects of pesticides on insect immunity is reviewed here. A basic understanding of these interactions is needed for several reasons, including to improve methods for controlling pest insects in agricultural settings, for controlling insect vectors of human diseases, and for reducing morta...

  1. Detection of insects in grain

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Detecting insects hidden inside kernels of grain is important to grain buyers because internal infestations can result in insect fragments in products made from the grain, or, if the grain is stored before use, the insect population can increase and damage the grain further. In a study in the Unite...

  2. Pathogen growth in insect hosts: inferring the importance of different mechanisms using stochastic models and response-time data.

    PubMed

    Kennedy, David A; Dukic, Vanja; Dwyer, Greg

    2014-09-01

    Pathogen population dynamics within individual hosts can alter disease epidemics and pathogen evolution, but our understanding of the mechanisms driving within-host dynamics is weak. Mathematical models have provided useful insights, but existing models have only rarely been subjected to rigorous tests, and their reliability is therefore open to question. Most models assume that initial pathogen population sizes are so large that stochastic effects due to small population sizes, so-called demographic stochasticity, are negligible, but whether this assumption is reasonable is unknown. Most models also assume that the dynamic effects of a host's immune system strongly affect pathogen incubation times or "response times," but whether such effects are important in real host-pathogen interactions is likewise unknown. Here we use data for a baculovirus of the gypsy moth to test models of within-host pathogen growth. By using Bayesian statistical techniques and formal model-selection procedures, we are able to show that the response time of the gypsy moth virus is strongly affected by both demographic stochasticity and a dynamic response of the host immune system. Our results imply that not all response-time variability can be explained by host and pathogen variability, and that immune system responses to infection may have important effects on population-level disease dynamics. PMID:25141148

  3. Relative importance of water chemistry and habitat to fish communities in headwater streams influenced by agricultural land use

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Channelized headwater streams are common throughout agricultural watersheds in the Midwestern United States. Understanding the relative impacts of agricultural contaminants and habitat degradation on the aquatic biota within agricultural headwater streams will provide information that can assist wi...

  4. Feeding the insect industry

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This article reports the use of insect colloidal artificial diets suitable for the rearing of economically important arthropods, such as Lygus lineolaris, Lygus hesperus, Coleomegilla maculata, and Phytoseiulus persimilis The different diets contain key nutrients such as proteins, carbohydrates, vit...

  5. U.S. Irrigation. Extent and Economic Importance. Agriculture Information Bulletin Number 523.

    ERIC Educational Resources Information Center

    Day, John C.; Horner, Gerald L.

    Data for the years 1974, 1978, 1982, and 1984 are used to identify the principal features of irrigated farming in the United States and to assess the importance of irrigation to the farm economy. Irrigation of U.S. acreage declined 5.6 million acres between 1978 and 1984 to 44.7 million acres. In 1982 irrigated acreage represented 6 percent of the…

  6. Comparisons of diazotrophic communities in native and agricultural desert ecosystems reveal plants as important drivers in diversity.

    PubMed

    Köberl, Martina; Erlacher, Armin; Ramadan, Elshahat M; El-Arabi, Tarek F; Müller, Henry; Bragina, Anastasia; Berg, Gabriele

    2016-02-01

    Diazotrophs provide the only biological source of fixed atmospheric nitrogen in the biosphere. Although they are the key player for plant-available nitrogen, less is known about their diversity and potential importance in arid ecosystems. We investigated the nitrogenase gene diversity in native and agricultural desert soil as well as within root-associated microbiota of medicinal plants grown in Egypt through the combination of nifH-specific qPCR, fingerprints, amplicon pyrosequencing and fluorescence in situ hybridization-confocal laser scanning microscopy. Although the diazotrophic microbiota were characterized by generally high abundances and diversity, statistically significant differences were found between both soils, the different microhabitats, and between the investigated plants (Matricaria chamomilla L., Calendula officinalis L. and Solanum distichum Schumach. and Thonn.). We observed a considerable community shift from desert to agriculturally used soil that demonstrated a higher abundance and diversity in the agro-ecosystem. The endorhiza was characterized by lower abundances and only a subset of species when compared to the rhizosphere. While the microbiomes of the Asteraceae were similar and dominated by potential root-nodulating rhizobia acquired primarily from soil, the perennial S. distichum generally formed associations with free-living nitrogen fixers. These results underline the importance of diazotrophs in desert ecosystems and additionally identify plants as important drivers in functional gene pool diversity. PMID:26705571

  7. Comparisons of diazotrophic communities in native and agricultural desert ecosystems reveal plants as important drivers in diversity

    PubMed Central

    Köberl, Martina; Erlacher, Armin; Ramadan, Elshahat M.; El-Arabi, Tarek F.; Müller, Henry; Bragina, Anastasia; Berg, Gabriele

    2015-01-01

    Diazotrophs provide the only biological source of fixed atmospheric nitrogen in the biosphere. Although they are the key player for plant-available nitrogen, less is known about their diversity and potential importance in arid ecosystems. We investigated the nitrogenase gene diversity in native and agricultural desert soil as well as within root-associated microbiota of medicinal plants grown in Egypt through the combination of nifH-specific qPCR, fingerprints, amplicon pyrosequencing and fluorescence in situ hybridization–confocal laser scanning microscopy. Although the diazotrophic microbiota were characterized by generally high abundances and diversity, statistically significant differences were found between both soils, the different microhabitats, and between the investigated plants (Matricaria chamomilla L., Calendula officinalis L. and Solanum distichum Schumach. and Thonn.). We observed a considerable community shift from desert to agriculturally used soil that demonstrated a higher abundance and diversity in the agro-ecosystem. The endorhiza was characterized by lower abundances and only a subset of species when compared to the rhizosphere. While the microbiomes of the Asteraceae were similar and dominated by potential root-nodulating rhizobia acquired primarily from soil, the perennial S. distichum generally formed associations with free-living nitrogen fixers. These results underline the importance of diazotrophs in desert ecosystems and additionally identify plants as important drivers in functional gene pool diversity. PMID:26705571

  8. Chitinases: in agriculture and human healthcare.

    PubMed

    Nagpure, Anand; Choudhary, Bharti; Gupta, Rajinder K

    2014-09-01

    Biological control of phytopathogenic fungi and insects continues to inspire the research and development of environmentally friendly bioactive alternatives. Potentially lytic enzymes, chitinases can act as a biocontrol agent against agriculturally important fungi and insects. The cell wall in fungi and protective covers, i.e. cuticle in insects shares a key structural polymer, chitin, a β-1,4-linked N-acetylglucosamine polymer. Therefore, it is advantageous to develop a common biocontrol agent against both of these groups. As chitin is absent in plants and mammals, targeting its metabolism will signify an eco-friendly strategy for the control of agriculturally important fungi and insects but is innocuous to mammals, plants, beneficial insects and other organisms. In addition, development of chitinase transgenic plant varieties probably holds the most promising method for augmenting agricultural crop protection and productivity, when properly integrated into traditional systems. Recently, human proteins with chitinase activity and chitinase-like proteins were identified and established as biomarkers for human diseases. This review covers the recent advances of chitinases as a biocontrol agent and its various applications including preparation of medically important chitooligosaccharides, bioconversion of chitin as well as in implementing chitinases as diagnostic and prognostic markers for numerous diseases and the prospect of their future utilization. PMID:23859124

  9. Association of N2-fixing cyanobacteria and plants: towards novel symbioses of agricultural importance

    SciTech Connect

    Elhai, Jeff

    2001-06-25

    Some nitrogen-fixing cyanobacteria are able to form symbioses with a wide variety of plants. Nostoc 2S9B is unusual in its ability to infect the roots of wheat, raising the prospect of a productive association with an important crop plant. The goal of the project was to lay the groundwork for the use of novel associations between Nostoc and crops of agronomic importance, thereby reducing our reliance on nitrogenous fertilizer. Nostoc 2S9B was found to enter roots through mechanical damage of roots and reside primarily in intercellular spaces. The strain could also be incorporated into wheat calli grown in tissue culture. In both cases, the rate of nitrogen fixation by the cyanobacterium was higher than that of the same strain grown with no plant present. Artificial nodules induced by the action of hormone 2,4D were readily infected by Nostoc 2S9B, and the cyanobacteria within such nodules fixed nitrogen under fully aerobic conditions. The nitrogen fixed was shown to be incorporated into the growing wheat seedlings. Nostoc thus differs from other bacteria in its ability to fix nitrogen in para-nodules without need for artificially microaerobic conditions. It would be useful to introduce foreign DNA into Nostoc 2S9B in order to make defined mutations to understand the genetic basis of its ability to infect wheat and to create strains that might facilitate the study of the infection process. Transfer of DNA into the cyanobacterium appears to be limited by the presence of four restriction enzymes, with recognition sequences the same as BamHI, BglI, BsaHI, and Tth111I. Genes encoding methyltransferases that protect DNA against these four enzymes have been cloned into helper plasmids to allow transfer of DNA from E. coli to Nostoc 2S9B.

  10. Insect Allergy.

    PubMed

    Lee, Hobart; Halverson, Sara; Mackey, Regina

    2016-09-01

    Insect bites and stings are common. Risk factors are mostly associated with environmental exposure. Most insect bites and stings result in mild, local, allergic reactions. Large local reactions and systemic reactions like anaphylaxis are possible. Common insects that bite or sting include mosquitoes, ticks, flies, fleas, biting midges, bees, and wasps. The diagnosis is made clinically. Identification of the insect should occur when possible. Management is usually supportive. For anaphylaxis, patients should be given epinephrine and transported to the emergency department for further evaluation. Venom immunotherapy (VIT) has several different protocols. VIT is highly effective in reducing systemic reactions and anaphylaxis. PMID:27545732

  11. Cadmium contamination in Tianjin agricultural soils and sediments: relative importance of atmospheric deposition from coal combustion.

    PubMed

    Wu, Guanghong; Yang, Cancan; Guo, Lan; Wang, Zhongliang

    2013-06-01

    Cadmium (Cd) in coal, fly ash, slag, atmospheric deposition, soils and sediments collected from Tianjin, northern China, were measured to provide baseline information and determine possible Cd sources and potential risk. The concentrations of Cd in coal, fly ash and atmospheric deposition were much higher than the soil background values. Fallout from coal-fired thermal power plants, heating boilers and industrial furnaces has increased the Cd concentration in soils and sediments in Tianjin. The concentrations of Cd in soils of suburban areas were significantly higher than in rural areas, suggesting that coal burning in Tianjin may have an important impact on the local physical environment. Cd from coal combustion is readily mobilized in soils. It is soluble and can form aqueous complexes and permeate river sediments. The high proportion of mobile Cd affects the migration of Cd in soils and sediments, which may pose an environmental threat in Tianjin due to the exposure to Cd and Cd compounds via the food chain. This study may provide a window for understanding and tracing sources of Cd in the local environment and the risk associated with Cd bioaccessibility. PMID:23212535

  12. Bovine tuberculosis (Mycobacterium bovis) in British farmland wildlife: the importance to agriculture.

    PubMed

    Mathews, Fiona; Macdonald, David W; Taylor, G Michael; Gelling, Merryl; Norman, Rachel A; Honess, Paul E; Foster, Rebecca; Gower, Charlotte M; Varley, Susan; Harris, Audrey; Palmer, Simonette; Hewinson, Glyn; Webster, Joanne P

    2006-02-01

    Bovine tuberculosis (bTB) is an important disease of cattle and an emerging infectious disease of humans. Cow- and badger-based control strategies have failed to eradicate bTB from the British cattle herd, and the incidence is rising by about 18%per year. The annual cost to taxpayers in Britain is currently 74 million UK pounds. Research has focused on the badger as a potential bTB reservoir, with little attention being paid to other mammals common on farmland. We have conducted a systematic survey of wild mammals (n=4393 individuals) present on dairy farms to explore the role of species other than badgers in the epidemiology of bTB. Cultures were prepared from 10397 samples (primarily faeces, urine and tracheal aspirates). One of the 1307 bank voles (Clethrionomys glareolus) live-sampled, and three of the 43 badgers (Meles meles), yielded positive isolates of Mycobacterium bovis. This is the first time the bacterium has been isolated from the bank vole. The strain type was the same as that found in cattle and badgers on the same farm. However, our work indicates that the mean prevalence of infectious individuals among common farmland wildlife is extremely low (the upper 95% confidence interval is < or =2.0 for all of the abundant species). Mathematical models illustrate that it is highly unlikely the disease could be maintained at such low levels. Our results suggest that these animals are relatively unimportant as reservoirs of bTB, having insufficient within-species (or within-group) transmission to sustain the infection, though occasional spill-overs from cattle or badgers may occur. PMID:16543179

  13. Importance of agricultural landscapes to nesting burrowing owls in the Northern Great Plains, USA

    USGS Publications Warehouse

    Restani, M.; Davies, J.M.; Newton, W.E.

    2008-01-01

    Anthropogenic habitat loss and fragmentation are the principle factors causing declines of grassland birds. Declines in burrowing owl (Athene cunicularia) populations have been extensive and have been linked to habitat loss, primarily the decline of black-tailed prairie dog (Cynomys ludovicianus) colonies. Development of habitat use models is a research priority and will aid conservation of owls inhabiting human-altered landscapes. From 2001 to 2004 we located 160 burrowing owl nests on prairie dog colonies on the Little Missouri National Grassland in North Dakota. We used multiple linear regression and Akaike's Information Criterion to estimate the relationship between cover type characteristics surrounding prairie dog colonies and (1) number of owl pairs per colony and (2) reproductive success. Models were developed for two spatial scales, within 600 m and 2,000 m radii of nests for cropland, crested wheatgrass (Agropyron cristatum), grassland, and prairie dog colonies. We also included number of patches as a metric of landscape fragmentation. Annually, fewer than 30% of prairie dog colonies were occupied by owls. None of the models at the 600 m scale explained variation in number of owl pairs or reproductive success. However, models at the 2,000 m scale did explain number of owl pairs and reproductive success. Models included cropland, crested wheatgrass, and prairie dog colonies. Grasslands were not included in any of the models and had low importance values, although percentage grassland surrounding colonies was high. Management that protects prairie dog colonies bordering cropland and crested wheatgrass should be implemented to maintain nesting habitat of burrowing owls. ?? 2008 Springer Science+Business Media B.V.

  14. Biological control of pests and insects. (Latest citations from the NTIS bibliographic database). Published Search

    SciTech Connect

    1995-02-01

    The bibliography contains citations concerning the use of biological agents to control insects and pests. Radiation, genetic breeding, bacteria, fungi, viruses, and pheromones are discussed as alternatives to pesticidal management. Methods for monitoring the effectiveness and environmental impact of these agents are reviewed. Population control of fruit flies, spruce sawflies, flies, mosquitoes, cockroaches, gypsy moths, and other agriculturally-important insects is also discussed. (Contains a minimum of 190 citations and includes a subject term index and title list.)

  15. Biological control of pests and insects. (Latest citations from the NTIS bibliographic database). Published Search

    SciTech Connect

    1996-04-01

    The bibliography contains citations concerning the use of biological agents to control insects and pests. Radiation, genetic breeding, bacteria, fungi, viruses, and pheromones are discussed as alternatives to pesticidal management. Methods for monitoring the effectiveness and environmental impact of these agents are reviewed. Population control of fruit flies, spruce sawflies, flies, mosquitoes, cockroaches, gypsy moths, and other agriculturally-important insects is also discussed. (Contains 50-250 citations and includes a subject term index and title list.) (Copyright NERAC, Inc. 1995)

  16. [Rats and dogs: important vectors of leptospirosis in agricultural areas in Cuidad Guzmán, Jalisco].

    PubMed

    Montes, Adan Sepúlveda; Dimas, Jorge Santiago; Preciado Rodríguez, Francisco Javier

    2002-01-01

    A study was conducted to determine how important rats and dogs are in the dissemination of leptospirosis in farms and stales located in Ciudad Guzmán, Jalisco. Thirteen of Leptospira interrograns serovarieties were used in microagglutination test of serogroups (MAT). Three hundred and fifty four rats (Rattus rattus and Rattus norvergicus) trapped in agricultural areas of the place were studied; 22 were positive (6,2%) and 34 suspected of leptospirosis (9,6%). Also four hundred and nineteen dogs from the region were tested; 22,6 % (95 dogs) were positive and 5,7%(24) suspected of L. Interrogans. These results showed that both species are important in the dissemination of the disease. PMID:15846935

  17. Community composition and population genetics of insect pathogenic fungi in the genus Metarhizium from soils of a long-term agricultural research system

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fungi in the genus Metarhizium are facultative pathogens of insects with the capacity to function in other niches, including soil and plant rhizosphere habitats. In agroecosystems, cropping and tillage practices heavily influence soil fungal communities with unknown effects on the distribution of M...

  18. Insect Keepers

    ERIC Educational Resources Information Center

    Moore, Virginia J.; Chessin, Debby A.; Theobald, Becky

    2010-01-01

    Insects are fascinating creatures--especially when you and your students get up close and personal with them! To that end, the authors facilitated an inquiry-based investigation with an emphasis on identification of the different types of insects found in the school yard, their characteristics, their habitat, and what they eat, while engaging the…

  19. Incredible Insects.

    ERIC Educational Resources Information Center

    Braus, Judy, Ed.

    1989-01-01

    Ranger Rick's NatureScope is a creative education series dedicated to inspiring in children an understanding and appreciation of the natural world while developing the skills they will need to make responsible decisions about the environment. Contents are organized into the following sections: (1) "What Makes an Insect an Insect?," including…

  20. Undergraduates' mental models about insect anatomy and insect life cycles

    NASA Astrophysics Data System (ADS)

    Diaz, Arlene Edith

    Educational studies focused on students' alternative conceptions have shown the importance of developing strategies to correct understanding. Identifying and comprehending student mental models are important since they may reflect alternate conceptions about scientific concepts. Mental models have been identified in various science education studies, but little is known about mental models undergraduates hold about insects. This research is significant because it identified mental models undergraduates have about insect anatomy and insect life cycles, exposed students to cognitive conflict by having them complete an online insect tutorial, and analyzed the effectiveness of this insect tutorial in correcting student understanding. An insect assessment was developed and administered pre- and post-instruction to probe students' mental models about insects. Different numbers of undergraduate students participated in different parts of the assessment; 276, 249, 166, and 58 students participated in the listing, drawing. definition, and life cycle parts of the assessment, respectively. The tutorial contained a variety of manipulated insect and non-insect images that challenged the students' understanding and generated cognitive conflict. This intervention guided students in replacing alternate conceptions with correct understanding. It was hypothesized that the tutorial would have a positive impact on student learning about insects. The results suggest that the tutorial had a positive impact on learning.

  1. Irradiation of northwest agricultural products

    NASA Astrophysics Data System (ADS)

    Eakin, D. E.; Tingey, G. I.

    1985-02-01

    Irradiation of food for disinfestation and preservation is increasing in importance because of increasing restrictions on various chemical treatments. Irradiation treatment is of particular interest in the Northwest because of a growing supply of agricultural products and the need to develop new export markets. Several products have, or could potentially have, significant export markets if stringent insect ocntrol procedures are developed and followed. Due to the recognized potential benefits of irradiation, this program was conducted to evaluate the benefits of using irradiation on Northwest agricultural products. Commodities currently included in the program are cherries, apples, asparagus, spices, hay, and hides.

  2. The Importance of Being Colorful and Able to Fly: Interpretation and Implications of Children's Statements on Selected Insects and Other Invertebrates

    ERIC Educational Resources Information Center

    Breuer, Gabriele B.; Schlegel, Jürg; Kauf, Peter; Rupf, Reto

    2015-01-01

    Children have served as research subjects in several surveys on attitudes to insects and invertebrates. Most of the studies have used quantitative scoring methods to draw conclusions. This paper takes a different approach as it analyzes children's free-text comments to gain an understanding of their viewpoints. A total of 246 children aged 9-13…

  3. Characterization of a novel insect-specific flavivirus from Brazil: Potential for inhibition of infection of arthropod cells with medically important flaviviruses.

    DOE PAGESBeta

    Kenney, Joan L.; Solberg, Owen D.; Langevin, Stanley A.; Brault, Aaron C.

    2014-01-12

    In the past decade, there has been an upsurge in the number of newly described insect-specific flaviviruses isolated pan-globally. We recently described the isolation of a novel flavivirus (tentatively designated ‘Nhumirim virus’; NHUV) that represents an example of a unique subset of apparently insect-specific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses despite appearing to be limited to replication in mosquito cells. We characterized the in vitro growth potential and 3' untranslated region (UTR) sequence homology with alternative flaviviruses, and evaluated the virus’s capacity to suppress replication of representative Culex spp.-vectored pathogenic flaviviruses in mosquito cells. Only mosquito cell linesmore » were found to support NHUV replication, further reinforcing the insect-specific phenotype of this virus. Analysis of the sequence and predicted RNA secondary structures of the 3' UTR indicated NHUV to be most similar to viruses within the yellow fever serogroup and Japanese encephalitis serogroup, and viruses in the tick-borne flavivirus clade. NHUV was found to share the fewest conserved sequence elements when compared with traditional insect-specific flaviviruses. This suggests that, despite apparently being insect specific, this virus probably diverged from an ancestral mosquito-borne flavivirus. Co-infection experiments indicated that prior or concurrent infection of mosquito cells with NHUV resulted in a significant reduction in virus production of West Nile virus (WNV), St Louis encephalitis virus (SLEV) and Japanese encephalitis virus. As a result, the inhibitory effect was most effective against WNV and SLEV with over a 106-fold and 104-fold reduction in peak titres, respectively.« less

  4. Characterization of a novel insect-specific flavivirus from Brazil: Potential for inhibition of infection of arthropod cells with medically important flaviviruses.

    SciTech Connect

    Kenney, Joan L.; Solberg, Owen D.; Langevin, Stanley A.; Brault, Aaron C.

    2014-01-12

    In the past decade, there has been an upsurge in the number of newly described insect-specific flaviviruses isolated pan-globally. We recently described the isolation of a novel flavivirus (tentatively designated ‘Nhumirim virus’; NHUV) that represents an example of a unique subset of apparently insect-specific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses despite appearing to be limited to replication in mosquito cells. We characterized the in vitro growth potential and 3' untranslated region (UTR) sequence homology with alternative flaviviruses, and evaluated the virus’s capacity to suppress replication of representative Culex spp.-vectored pathogenic flaviviruses in mosquito cells. Only mosquito cell lines were found to support NHUV replication, further reinforcing the insect-specific phenotype of this virus. Analysis of the sequence and predicted RNA secondary structures of the 3' UTR indicated NHUV to be most similar to viruses within the yellow fever serogroup and Japanese encephalitis serogroup, and viruses in the tick-borne flavivirus clade. NHUV was found to share the fewest conserved sequence elements when compared with traditional insect-specific flaviviruses. This suggests that, despite apparently being insect specific, this virus probably diverged from an ancestral mosquito-borne flavivirus. Co-infection experiments indicated that prior or concurrent infection of mosquito cells with NHUV resulted in a significant reduction in virus production of West Nile virus (WNV), St Louis encephalitis virus (SLEV) and Japanese encephalitis virus. As a result, the inhibitory effect was most effective against WNV and SLEV with over a 106-fold and 104-fold reduction in peak titres, respectively.

  5. Characterization of a novel insect-specific flavivirus from Brazil: potential for inhibition of infection of arthropod cells with medically important flaviviruses

    PubMed Central

    Kenney, Joan L.; Solberg, Owen D.; Langevin, Stanley A.; Brault, Aaron C.

    2015-01-01

    In the past decade there has been an upsurge in the number of newly described insect-specific flaviviruses isolated pan-globally. We recently described the isolation of a novel flavivirus (tentatively designated “Nhumirim virus”; NHUV) (Pauvolid-Correa et al., in review) that represents an example of a unique subset of apparently insect-specific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses despite appearing to be limited to replication in mosquito cells. We characterized the in vitro growth potential, 3’ untranslated region (UTR) sequence homology with alternative flaviviruses, and evaluated the virus’s capacity to suppress replication of representative Culex spp. vectored pathogenic flaviviruses in mosquito cells. Only mosquito cell lines were found to support NHUV replication, further reinforcing the insect-specific phenotype of this virus. Analysis of the sequence and predicted RNA secondary structures of the 3’ UTR indicate NHUV to be most similar to viruses within the yellow fever serogroup, Japanese encephalitis serogroup, and viruses in the tick-borne flavivirus clade. NHUV was found to share the fewest conserved sequence elements when compared to traditional insect-specific flaviviruses. This suggests that, despite being apparently insect-specific, this virus likely diverged from an ancestral mosquito-borne flavivirus. Co-infection experiments indicated that prior or concurrent infection of mosquito cells with NHUV resulted in significant reduction in viral production of West Nile virus (WNV), St. Louis encephalitis virus (SLEV) and Japanese encephalitis virus. The inhibitory effect was most effective against WNV and SLEV with over a million-fold and 10,000-fold reduction in peak titers, respectively. PMID:25146007

  6. CHARACTERIZATION OF MICROBIAL GUT FLORA OF HETEROPTEROUS INSECTS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Many insects harbor a robust complement of prokaryotes in their alimentary canals. These microorganisms may facilitate nutrient availability and utilization, detoxification of environmental toxins, or play other important roles in the insect's life history. Understanding insect-microorganism inter...

  7. Identification and characterization of insect-specific proteins by genome data analysis

    PubMed Central

    Zhang, Guojie; Wang, Hongsheng; Shi, Junjie; Wang, Xiaoling; Zheng, Hongkun; Wong, Gane Ka-Shu; Clark, Terry; Wang, Wen; Wang, Jun; Kang, Le

    2007-01-01

    Background Insects constitute the vast majority of known species with their importance including biodiversity, agricultural, and human health concerns. It is likely that the successful adaptation of the Insecta clade depends on specific components in its proteome that give rise to specialized features. However, proteome determination is an intensive undertaking. Here we present results from a computational method that uses genome analysis to characterize insect and eukaryote proteomes as an approximation complementary to experimental approaches. Results Homologs in common to Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Tribolium castaneum, and Apis mellifera were compared to the complete genomes of three non-insect eukaryotes (opisthokonts) Homo sapiens, Caenorhabditis elegans and Saccharomyces cerevisiae. This operation yielded 154 groups of orthologous proteins in Drosophila to be insect-specific homologs; 466 groups were determined to be common to eukaryotes (represented by three opisthokonts). ESTs from the hemimetabolous insect Locust migratoria were also considered in order to approximate their corresponding genes in the insect-specific homologs. Stress and stimulus response proteins were found to constitute a higher fraction in the insect-specific homologs than in the homologs common to eukaryotes. Conclusion The significant representation of stress response and stimulus response proteins in proteins determined to be insect-specific, along with specific cuticle and pheromone/odorant binding proteins, suggest that communication and adaptation to environments may distinguish insect evolution relative to other eukaryotes. The tendency for low Ka/Ks ratios in the insect-specific protein set suggests purifying selection pressure. The generally larger number of paralogs in the insect-specific proteins may indicate adaptation to environment changes. Instances in our insect-specific protein set have been arrived at through experiments reported in the

  8. Community composition and population genetics of insect pathogenic fungi in the genus Metarhizium from soils of a long-term agricultural research system.

    PubMed

    Kepler, Ryan M; Ugine, Todd A; Maul, Jude E; Cavigelli, Michel A; Rehner, Stephen A

    2015-08-01

    Fungi in the genus Metarhizium are insect pathogens able to function in other niches, including soil and plant rhizosphere habitats. In agroecosystems, cropping and tillage practices influence soil fungal communities with unknown effects on the distribution of Metarhizium, whose presence can reduce populations of crop pests. We report results from a selective media survey of Metarhizium in soils sampled from a long-term experimental farming project in the mid-Atlantic region. Field plots under soybean cultivation produced higher numbers of Metarhizium colony-forming units (cfu) than corn or alfalfa. Plots managed organically and via chisel-till harboured higher numbers of Metarhizium cfu than no-till plots. Sequence typing of Metarhizium isolates revealed four species, with M. robertsii and M. brunneum predominating. The M. brunneum population was essentially fixed for a single clone as determined by multilocus microsatellite genotyping. In contrast, M. robertsii was found to contain significant diversity, with the majority of isolates distributed between two principal clades. Evidence for recombination was observed only in the most abundant clade. These findings illuminate multiple levels of Metarhizium diversity that can be used to inform strategies by which soil Metarhizium populations may be manipulated to exert downward pressure on pest insects and promote plant health. PMID:25627647

  9. Climate impacts on European agriculture and water management in the context of adaptation and mitigation--the importance of an integrated approach.

    PubMed

    Falloon, Pete; Betts, Richard

    2010-11-01

    We review and qualitatively assess the importance of interactions and feedbacks in assessing climate change impacts on water and agriculture in Europe. We focus particularly on the impact of future hydrological changes on agricultural greenhouse gas (GHG) mitigation and adaptation options. Future projected trends in European agriculture include northward movement of crop suitability zones and increasing crop productivity in Northern Europe, but declining productivity and suitability in Southern Europe. This may be accompanied by a widening of water resource differences between the North and South, and an increase in extreme rainfall events and droughts. Changes in future hydrology and water management practices will influence agricultural adaptation measures and alter the effectiveness of agricultural mitigation strategies. These interactions are often highly complex and influenced by a number of factors which are themselves influenced by climate. Mainly positive impacts may be anticipated for Northern Europe, where agricultural adaptation may be shaped by reduced vulnerability of production, increased water supply and reduced water demand. However, increasing flood hazards may present challenges for agriculture, and summer irrigation shortages may result from earlier spring runoff peaks in some regions. Conversely, the need for effective adaptation will be greatest in Southern Europe as a result of increased production vulnerability, reduced water supply and increased demands for irrigation. Increasing flood and drought risks will further contribute to the need for robust management practices. The impacts of future hydrological changes on agricultural mitigation in Europe will depend on the balance between changes in productivity and rates of decomposition and GHG emission, both of which depend on climatic, land and management factors. Small increases in European soil organic carbon (SOC) stocks per unit land area are anticipated considering changes in climate

  10. The Importance of Being Colorful and Able to Fly: Interpretation and implications of children's statements on selected insects and other invertebrates

    NASA Astrophysics Data System (ADS)

    Breuer, Gabriele B.; Schlegel, Jürg; Kauf, Peter; Rupf, Reto

    2015-11-01

    Children have served as research subjects in several surveys on attitudes to insects and invertebrates. Most of the studies have used quantitative scoring methods to draw conclusions. This paper takes a different approach as it analyzes children's free-text comments to gain an understanding of their viewpoints. A total of 246 children aged 9-13 completed a standard questionnaire regarding their attitudes toward 18 invertebrates indigenous to Switzerland. Fourteen insect species and four other invertebrates were individually presented in a color photograph without any further background information. The children were given the opportunity to provide comments on each animal to explain the attitude score they had awarded. Nearly 5,000 comments were coded and categorized into 7 positive and 9 negative categories. A significant correlation between fear and disgust was not detected. Based on a hierarchical cluster analysis, we concluded that flying in the air versus crawling on the ground was a major differentiator for attitude and underlying reasons, only being trumped by the fear of getting stung. The visualization of our findings in a cluster heat map provided further insights into shared statement categories by species. Our analysis establishes that fear and disgust are separate emotions with regard to insects and other invertebrates. Based on our findings, we believe that prejudice-based fear and culturally evolved revulsion can be overcome. We suggest promoting environmental education programs, especially if they allow for personal experience, provide information in emotion-activating formats, and include content that resolves existing misinformation and myths.

  11. Beneficial Insects and Spiders of Alaska

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The development of integrated pest management programs is dependent on the availability of biological information on beneficial insects and natural enemies of agricultural pests. This cooperative effort between ARS and UAF represents the first manual on beneficial insects and natural enemies of pest...

  12. [Effect of transgenic insect-resistant rice on biodiversity].

    PubMed

    Zhang, Lei; Zhu, Zhen

    2011-05-01

    Rice is the most important food crops in maintaining food security in China. The loss of China's annual rice production caused by pests is over ten million tons. Present studies showed that the transgenic insect-resistant rice can substantially reduce the application amount of chemical pesticides. In the case of no pesticide use, the pest density in transgenic rice field is significantly lower than that in non-transgenic field, and the neutral insects and natural enemies of pests increased significantly, indicating that the ecological environment and biodiversity toward the positive direction. The gene flow frequency from transgenic rice is dramatically reduced with the distance increases, reaching less than 0.01% at the distance of 6.2 m. Application of transgenic insect-resistant rice in China has an important significance for ensuring food security, maintaining sustainable agricultural development, and protecting the ecological environment and biodiversity. This review summarized the research progress in transgenic insect-resistant rice and its effect on biodiversity. The research directions and development trends of crop pest controlling in future are discussed. These help to promote better use of transgenic insect-resistant rice. PMID:21586387

  13. Insect bite reactions.

    PubMed

    Singh, Sanjay; Mann, Baldeep Kaur

    2013-01-01

    Insects are a class of living creatures within the arthropods. Insect bite reactions are commonly seen in clinical practice. The present review touches upon the medically important insects and their places in the classification, the sparse literature on the epidemiology of insect bites in India, and different variables influencing the susceptibility of an individual to insect bites. Clinical features of mosquito bites, hypersensitivity to mosquito bites Epstein-Barr virus NK (HMB-EBV-NK) disease, eruptive pseudoangiomatosis, Skeeter syndrome, papular pruritic eruption of HIV/AIDS, and clinical features produced by bed bugs, Mexican chicken bugs, assassin bugs, kissing bugs, fleas, black flies, Blandford flies, louse flies, tsetse flies, midges, and thrips are discussed. Brief account is presented of the immunogenic components of mosquito and bed bug saliva. Papular urticaria is discussed including its epidemiology, the 5 stages of skin reaction, the SCRATCH principle as an aid in diagnosis, and the recent evidence supporting participation of types I, III, and IV hypersensitivity reactions in its causation is summarized. Recent developments in the treatment of pediculosis capitis including spinosad 0.9% suspension, benzyl alcohol 5% lotion, dimethicone 4% lotion, isopropyl myristate 50% rinse, and other suffocants are discussed within the context of evidence derived from randomized controlled trials and key findings of a recent systematic review. We also touch upon a non-chemical treatment of head lice and the ineffectiveness of egg-loosening products. Knockdown resistance (kdr) as the genetic mechanism making the lice nerves insensitive to permethrin is discussed along with the surprising contrary clinical evidence from Europe about efficacy of permethrin in children with head lice carrying kdr-like gene. The review also presents a brief account of insects as vectors of diseases and ends with discussion of prevention of insect bites and some serious adverse effects

  14. Peripheral olfactory signaling in insects

    PubMed Central

    Suh, Eunho; Bohbot, Jonathan; Zwiebel, Laurence J.

    2014-01-01

    Olfactory signaling is a crucial component in the life history of insects. The development of precise and parallel mechanisms to analyze the tremendous amount of chemical information from the environment and other sources has been essential to their evolutionary success. Considerable progress has been made in the study of insect olfaction fueled by bioinformatics- based utilization of genomics along with rapid advances in functional analyses. Here we review recent progress in our rapidly emerging understanding of insect peripheral sensory reception and signal transduction. These studies reveal that the nearly unlimited chemical space insects encounter is covered by distinct chemosensory receptor repertoires that are generally derived by species-specific, rapid gene gain and loss, reflecting the evolutionary consequences of adaptation to meet their specific biological needs. While diverse molecular mechanisms have been put forth, often in the context of controversial models, the characterization of the ubiquitous, highly conserved and insect-specific Orco odorant receptor co-receptor has opened the door to the design and development of novel insect control methods to target agricultural pests, disease vectors and even nuisance insects. PMID:25584200

  15. The stock of invasive insect species and its economic determinants.

    PubMed

    Hlasny, Vladimir

    2011-06-01

    Invasions of nonindigenous organisms have long been linked to trade, but the contribution of individual trade pathways remains poorly understood, because species are not observed immediately upon arrival and the number of species arriving annually is unknown. Species interception records may count both new arrivals and species long introduced. Furthermore, the stock of invasive insect species already present is unknown. In this study, a state-space model is used to infer the stock of detected as well as undetected invasive insect species established in the United States. A system of equations is estimated jointly to distinguish the patterns of introduction, identification, and eradication. Introductions of invasive species are modeled as dependent on the volume of trade and arrival of people. Identifications depend on the public efforts at invasive species research, as well as on the established stock of invasive species that remain undetected. Eradications of both detected and undetected invasive species depend on containment and quarantine efforts, as well as on the stock of all established invasive species. These patterns are estimated by fitting the predicted number of invasive species detections to the observed record in the North American Non-Indigenous Arthropod Database. The results indicate that agricultural imports are the most important pathway of introduction, followed by immigration of people. Expenditures by the U.S. Department of Agriculture and the Agricultural Research Service are found to explain the species identification record well. Between three and 38 invasive insect species are estimated to be established in the United States undetected. PMID:21735892

  16. 7 CFR 58.147 - Insect and rodent control program.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Insect and rodent control program. 58.147 Section 58... Service 1 Operations and Operating Procedures § 58.147 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control...

  17. 7 CFR 58.247 - Insect and rodent control program.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Insect and rodent control program. 58.247 Section 58... Service 1 Operations and Operating Procedures § 58.247 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control program...

  18. 7 CFR 58.247 - Insect and rodent control program.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Insect and rodent control program. 58.247 Section 58... Service 1 Operations and Operating Procedures § 58.247 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control program...

  19. 7 CFR 58.247 - Insect and rodent control program.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Insect and rodent control program. 58.247 Section 58... Service 1 Operations and Operating Procedures § 58.247 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control program...

  20. 7 CFR 58.247 - Insect and rodent control program.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Insect and rodent control program. 58.247 Section 58... Service 1 Operations and Operating Procedures § 58.247 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control program...

  1. 7 CFR 58.147 - Insect and rodent control program.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Insect and rodent control program. 58.147 Section 58... Service 1 Operations and Operating Procedures § 58.147 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control...

  2. 7 CFR 58.147 - Insect and rodent control program.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Insect and rodent control program. 58.147 Section 58... Service 1 Operations and Operating Procedures § 58.147 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control...

  3. 7 CFR 58.247 - Insect and rodent control program.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Insect and rodent control program. 58.247 Section 58... Service 1 Operations and Operating Procedures § 58.247 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control program...

  4. 7 CFR 58.147 - Insect and rodent control program.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Insect and rodent control program. 58.147 Section 58... Service 1 Operations and Operating Procedures § 58.147 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control...

  5. 7 CFR 58.147 - Insect and rodent control program.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Insect and rodent control program. 58.147 Section 58... Service 1 Operations and Operating Procedures § 58.147 Insect and rodent control program. In addition to... made responsible for the performance of a regularly scheduled insect and rodent control...

  6. Insects: A nutritional alternative

    NASA Technical Reports Server (NTRS)

    Dufour, P. A.

    1981-01-01

    Insects are considered as potential food sources in space. Types of insects consumed are discussed. Hazards of insect ingestion are considered. Insect reproduction, requirements, and raw materials conversion are discussed. Nutrition properties and composition of insects are considered. Preparation of insects as human food is discussed.

  7. [Impact of pesticides on biodiversity in agricultural areas].

    PubMed

    Wu, Chunhua; Chen, Xin

    2004-02-01

    Large amount application of pesticides caused a lot of ecological and environmental problems, among which, the impact of pesticides on biodiversity was most important. In this paper, an overview of the impacts of pesticides on biodiversity in agricultural areas, including the community structure of insects, populations of soil invertebrates and microorganisms, and plant communities was provided, and the reasonable use of pesticides and the measures of protecting biodiversity in agricultural areas were also put forward. PMID:15146653

  8. Habitat use and movement patterns of Northern Pintails during spring in northern Japan: the importance of agricultural lands

    USGS Publications Warehouse

    Yamaguchi, Noriyuki M.; Hupp, Jerry W.; Flint, Paul L.; Pearce, John M.; Shigeta, Yusuke; Shimada, Tetsuo; Hiraoka, Emiko N.; Higuchi, Hiroyoshi

    2012-01-01

    From 2006 to 2009, we marked 198 Northern Pintails (Anas acuta) with satellite transmitters on their wintering areas in Japan to study their migration routes and habitat use in spring staging areas. We hypothesized that the distribution of pintails during spring staging was influenced by patterns of land use and expected that the most frequently used areas would have more agricultural habitat than lesser-used areas. We obtained 3031 daily locations from 163 migrant pintails marked with satellite transmitters and identified 524 stopover sites. Based on a fixed kernel home range analysis of stopover utilization distribution (UD), core staging areas (areas within the 50% UD) were identified in northern Honshu and western Hokkaido, and were used by 71% of marked pintails. Core staging areas had a greater proportion of rice fields than peripheral (51–95% UD) and rarely used (outside the 95% UD) staging areas. Stopover sites also contained more rice fields and other agricultural land than were available at regional scales, indicating that pintails selected rice and other agricultural habitats at regional and local scales. Pintails remained at spring staging areas an average of 51 d. Prolonged staging in agricultural habitats of northern Japan was likely necessary for pintails to prepare for transoceanic migration to Arctic nesting areas in eastern Russia.

  9. An Examination of Important Competencies Necessary for Vocational Agriculture in Selected Senior Secondary Students in Ijebu North Local Government Area, Nigeria

    ERIC Educational Resources Information Center

    Onanuga, Peter Abayomi

    2015-01-01

    The study analyses the relationship existing between some specified competencies important to vocational agriculture and preparation for occupation between male and female students in senior secondary schools in Ijebu-North Local Government Area, Nigeria. It adopted the classical design for change experiment (i.e. before and after measures) with…

  10. Insect evolution.

    PubMed

    Engel, Michael S

    2015-10-01

    It goes without saying that insects epitomize diversity, and with over a million documented species they stand out as one of the most remarkable lineages in the 3.5-billion-year history of life on earth (Figure 1). This reality is passé to even the layperson and is taken for granted in the same way none of us think much of our breathing as we go about our day, and yet insects are just as vital to our existence. Insects are simultaneously familiar and foreign to us, and while a small fraction are beloved or reviled, most are simply ignored. These inexorable evolutionary overachievers outnumber us all, their segmented body plan is remarkably labile, they combine a capacity for high rates of speciation with low levels of natural extinction, and their history of successes eclipses those of the more familiar ages of dinosaurs and mammals alike. It is their evolution - persisting over vast expanses of geological time and inextricably implicated in the diversification of other lineages - that stands as one of the most expansive subjects in biology. PMID:26439349

  11. Environmental RNAi in herbivorous insects

    PubMed Central

    Ivashuta, Sergey; Zhang, Yuanji; Wiggins, B. Elizabeth; Ramaseshadri, Partha; Segers, Gerrit C.; Johnson, Steven; Meyer, Steve E.; Kerstetter, Randy A.; McNulty, Brian C.; Bolognesi, Renata; Heck, Gregory R.

    2015-01-01

    Environmental RNAi (eRNAi) is a sequence-specific regulation of endogenous gene expression in a receptive organism by exogenous double-stranded RNA (dsRNA). Although demonstrated under artificial dietary conditions and via transgenic plant presentations in several herbivorous insects, the magnitude and consequence of exogenous dsRNA uptake and the role of eRNAi remains unknown under natural insect living conditions. Our analysis of coleopteran insects sensitive to eRNAi fed on wild-type plants revealed uptake of plant endogenous long dsRNAs, but not small RNAs. Subsequently, the dsRNAs were processed into 21 nt siRNAs by insects and accumulated in high quantities in insect cells. No accumulation of host plant-derived siRNAs was observed in lepidopteran larvae that are recalcitrant to eRNAi. Stability of ingested dsRNA in coleopteran larval gut followed by uptake and transport from the gut to distal tissues appeared to be enabling factors for eRNAi. Although a relatively large number of distinct coleopteran insect-processed plant-derived siRNAs had sequence complementarity to insect transcripts, the vast majority of the siRNAs were present in relatively low abundance, and RNA-seq analysis did not detect a significant effect of plant-derived siRNAs on insect transcriptome. In summary, we observed a broad genome-wide uptake of plant endogenous dsRNA and subsequent processing of ingested dsRNA into 21 nt siRNAs in eRNAi-sensitive insects under natural feeding conditions. In addition to dsRNA stability in gut lumen and uptake, dosage of siRNAs targeting a given insect transcript is likely an important factor in order to achieve measurable eRNAi-based regulation in eRNAi-competent insects that lack an apparent silencing amplification mechanism. PMID:25802407

  12. Insect abatement system

    NASA Technical Reports Server (NTRS)

    Spiro, Clifford Lawrence (Inventor); Burnell, Timothy Brydon (Inventor); Wengrovius, Jeffrey Hayward (Inventor)

    1997-01-01

    An insect abatement system prevents adhesion of insect debris to surfaces which must be kept substantially free of insect debris. An article is coated with an insect abatement coating comprising polyorganosiloxane with a Shore A hardness of less than 50 and a tensile strength of less than 4 MPa. A method for preventing the adhesion of insect debris to surfaces includes the step of applying an insect abatement coating to a surface which must be kept substantially free of insect debris.

  13. Agroterrorism, biological crimes, and biowarfare targeting animal agriculture. The clinical, pathologic, diagnostic, and epidemiologic features of some important animal diseases.

    PubMed

    Wilson, T M; Gregg, D A; King, D J; Noah, D L; Perkins, L E; Swayne, D E; Inskeep, W

    2001-09-01

    In the past 100 years, to our knowledge there have been approximately 12 events involving the intentional introduction of microbiologic agents into livestock and animal populations worldwide, of which three were World War I events in the United States. To the best of the authors' knowledge, there has been no recent intentional introduction of microbiologic agents (viruses or bacteria) into livestock and animal populations in the United States. The criminal or terrorist use of chemicals against animals and agriculture products have been more common. With the political, economic, and military new world order, however, the United States must maintain a vigilant posture. The framework for this vigilance must be an intelligence system sensitive to the needs of agriculture and a first-class animal disease diagnostic surveillance and response system. PMID:11572141

  14. Impacts of transgenic poplar-cotton agro-ecosystems upon target pests and non-target insects under field conditions.

    PubMed

    Zhang, D J; Liu, J X; Lu, Z Y; Li, C L; Comada, E; Yang, M S

    2015-01-01

    Poplar-cotton agro-ecosystems are the main agricultural planting modes of cotton fields in China. With increasing acres devoted to transgenic insect-resistant poplar and transgenic insect-resistant cotton, studies examining the effects of transgenic plants on target and non-target insects become increasingly important. We systematically surveyed populations of both target pests and non-target insects for 4 different combinations of poplar-cotton eco-systems over 3 years. Transgenic Bt cotton strongly resisted the target insects Fall webworm moth [Hyphantria cunea (Drury)], Sylepta derogata Fabrieius, and American bollworm (Heliothis armigera), but no clear impact on non-target insect cotton aphids (Aphis gossypii). Importantly, intercrops containing transgenic Pb29 poplar significantly increased the inhibitory effects of Bt cotton on Fall webworm moth in ecosystem IV. Highly resistant Pb29 poplar reduced populations of the target pests Grnsonoma minutara Hubner and non-target insect poplar leaf aphid (Chaitophorus po-pulialbae), while Fall webworm moth populations were unaffected. We determined the effects of Bt toxin from transgenic poplar and cotton on target and non-target pests in different ecosystems of cotton-poplar intercrops and identified the synergistic effects of such combinations toward both target and non-target insects. PMID:26345739

  15. Promoting pollinating insects in intensive agricultural matrices: field-scale experimental manipulation of hay-meadow mowing regimes and its effects on bees.

    PubMed

    Buri, Pierrick; Humbert, Jean-Yves; Arlettaz, Raphaël

    2014-01-01

    Bees are a key component of biodiversity as they ensure a crucial ecosystem service: pollination. This ecosystem service is nowadays threatened, because bees suffer from agricultural intensification. Yet, bees rarely benefit from the measures established to promote biodiversity in farmland, such as agri-environment schemes (AES). We experimentally tested if the spatio-temporal modification of mowing regimes within extensively managed hay meadows, a widespread AES, can promote bees. We applied a randomized block design, replicated 12 times across the Swiss lowlands, that consisted of three different mowing treatments: 1) first cut not before 15 June (conventional regime for meadows within Swiss AES); 2) first cut not before 15 June, as treatment 1 but with 15% of area left uncut serving as a refuge; 3) first cut not before 15 July. Bees were collected with pan traps, twice during the vegetation season (before and after mowing). Wild bee abundance and species richness significantly increased in meadows where uncut refuges were left, in comparison to meadows without refuges: there was both an immediate (within year) and cumulative (from one year to the following) positive effect of the uncut refuge treatment. An immediate positive effect of delayed mowing was also evidenced in both wild bees and honey bees. Conventional AES could easily accommodate such a simple management prescription that promotes farmland biodiversity and is likely to enhance pollination services. PMID:24416434

  16. Microbial ecology-based methods to characterize the bacterial communities of non-model insects.

    PubMed

    Prosdocimi, Erica M; Mapelli, Francesca; Gonella, Elena; Borin, Sara; Crotti, Elena

    2015-12-01

    Among the animals of the Kingdom Animalia, insects are unparalleled for their widespread diffusion, diversity and number of occupied ecological niches. In recent years they have raised researcher interest not only because of their importance as human and agricultural pests, disease vectors and as useful breeding species (e.g. honeybee and silkworm), but also because of their suitability as animal models. It is now fully recognized that microorganisms form symbiotic relationships with insects, influencing their survival, fitness, development, mating habits and the immune system and other aspects of the biology and ecology of the insect host. Thus, any research aimed at deepening the knowledge of any given insect species (perhaps species of applied interest or species emerging as novel pests or vectors) must consider the characterization of the associated microbiome. The present review critically examines the microbiology and molecular ecology techniques that can be applied to the taxonomical and functional analysis of the microbiome of non-model insects. Our goal is to provide an overview of current approaches and methods addressing the ecology and functions of microorganisms and microbiomes associated with insects. Our focus is on operational details, aiming to provide a concise guide to currently available advanced techniques, in an effort to extend insect microbiome research beyond simple descriptions of microbial communities. PMID:26476138

  17. Beneficial Insect Attraction to Milkweeds (Asclepias speciosa, Asclepias fascicularis) in Washington State, USA.

    PubMed

    James, David G; Seymour, Lorraine; Lauby, Gerry; Buckley, Katie

    2016-01-01

    Native plant and beneficial insect associations are relatively unstudied yet are important in native habitat restoration programs for improving and sustaining conservation biological control of arthropod pests in agricultural crops. Milkweeds (Asclepias spp.) are currently the focus of restoration programs in the USA aimed at reversing a decline in populations of the milkweed-dependent monarch butterfly (Danaus plexippus); however, little is known of the benefits of these plants to other beneficial insects. Beneficial insects (predators, parasitoids, pollinators) attracted to two milkweed species (Asclepias speciosa, Asclepias fascicularis) in central Washington State, WA, USA were identified and counted on transparent sticky traps attached to blooms over five seasons. Combining all categories of beneficial insects, means of 128 and 126 insects per trap were recorded for A. speciosa and A. fascicularis, respectively. Predatory and parasitic flies dominated trap catches for A. speciosa while parasitic wasps were the most commonly trapped beneficial insects on A. fascicularis. Bees were trapped commonly on both species, especially A. speciosa with native bees trapped in significantly greater numbers than honey bees. Beneficial insect attraction to A. speciosa and A. fascicularis was substantial. Therefore, these plants are ideal candidates for habitat restoration, intended to enhance conservation biological control, and for pollinator conservation. In central Washington, milkweed restoration programs for enhancement of D. plexippus populations should also provide benefits for pest suppression and pollinator conservation. PMID:27367733

  18. Dynamic role and importance of surrogate species for assessing potential adverse environmental impacts of genetically engineered insect-resistant plants on non-target organisms.

    PubMed

    Wach, Michael; Hellmich, Richard L; Layton, Raymond; Romeis, Jörg; Gadaleta, Patricia G

    2016-08-01

    Surrogate species have a long history of use in research and regulatory settings to understand the potentially harmful effects of toxic substances including pesticides. More recently, surrogate species have been used to evaluate the potential effects of proteins contained in genetically engineered insect resistant (GEIR) crops. Species commonly used in GEIR crop testing include beneficial organisms such as honeybees, arthropod predators, and parasitoids. The choice of appropriate surrogates is influenced by scientific factors such as the knowledge of the mode of action and the spectrum of activity as well as societal factors such as protection goals that assign value to certain ecosystem services such as pollination or pest control. The primary reasons for using surrogates include the inability to test all possible organisms, the restrictions on using certain organisms in testing (e.g., rare, threatened, or endangered species), and the ability to achieve greater sensitivity and statistical power by using laboratory testing of certain species. The acceptance of surrogate species data can allow results from one region to be applied or "transported" for use in another region. On the basis of over a decade of using surrogate species to evaluate potential effects of GEIR crops, it appears that the current surrogates have worked well to predict effects of GEIR crops that have been developed (Carstens et al. GM Crops Food 5:1-5, 2014), and it is expected that they should work well to predict effects of future GEIR crops based on similar technologies. PMID:26922585

  19. Allergies to Insect Venom

    MedlinePlus

    ... The smell of food attracts these insects.  Use insect repellents and keep insecticide available. Treatment tips:  Venom immunotherapy (allergy shots to insect venom(s) is highly effective in preventing subsequent sting ...

  20. The importance of the riparian zone and in-stream processes in nitrate attenuation in undisturbed and agricultural watersheds – a review of the scientific literature

    USGS Publications Warehouse

    Ranalli, Anthony J.; MacAlady, Donald L.

    2010-01-01

    We reviewed published studies from primarily glaciated regions in the United States, Canada, and Europe of the (1) transport of nitrate from terrestrial ecosystems to aquatic ecosystems, (2) attenuation of nitrate in the riparian zone of undisturbed and agricultural watersheds, (3) processes contributing to nitrate attenuation in riparian zones, (4) variation in the attenuation of nitrate in the riparian zone, and (5) importance of in-stream and hyporheic processes for nitrate attenuation in the stream channel. Our objectives were to synthesize the results of these studies and suggest methodologies to (1) monitor regional trends in nitrate concentration in undisturbed 1st order watersheds and (2) reduce nitrate loads in streams draining agricultural watersheds. Our review reveals that undisturbed headwater watersheds have been shown to be very retentive of nitrogen, but the importance of biogeochemical and hydrological riparian zone processes in retaining nitrogen in these watersheds has not been demonstrated as it has for agricultural watersheds. An understanding of the role of the riparian zone in nitrate attenuation in undisturbed watersheds is crucial because these watersheds are increasingly subject to stressors, such as changes in land use and climate, wildfire, and increases in atmospheric nitrogen deposition. In general, understanding processes controlling the concentration and flux of nitrate is critical to identifying and mapping the vulnerability of watersheds to water quality changes due to a variety of stressors. In undisturbed and agricultural watersheds we propose that understanding the importance of riparian zone processes in 2nd order and larger watersheds is critical. Research is needed that addresses the relative importance of how the following sources of nitrate along any given stream reach might change as watersheds increase in size and with flow: (1) inputs upstream from the reach, (2) tributary inflow, (3) water derived from the riparian zone

  1. Agricultural Waste.

    PubMed

    Xue, Ling; Zhang, Panpan; Shu, Huajie; Chang, Chein-Chi; Wang, Renqing; Zhang, Shuping

    2016-10-01

    In recent years, the quantity of agricultural waste has been rising rapidly all over the world. As a result, the environmental problems and negative impacts of agricultural waste are drawn more and more attention. Therefore, there is a need to adopt proper approaches to reduce and reuse agricultural waste. This review presented about 200 literatures published in 2015 relating to the topic of agricultural waste. The review examined research on agricultural waste in 2015 from the following four aspects: the characterization, reuse, treatment, and management. Researchers highlighted the importance to reuse agricultural waste and investigated the potential to utilize it as biofertilizers, cultivation material, soil amendments, adsorbent, material, energy recycling, enzyme and catalyst etc. The treatment of agricultural waste included carbonization, biodegradation, composting hydrolysis and pyrolysis. Moreover, this review analyzed the differences of the research progress in 2015 from 2014. It may help to reveal the new findings and new trends in this field in 2015 comparing to 2014. PMID:27620093

  2. Insect transgenesis and the sterile insect technique

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The establishment of broadly applicable insect transgenesis systems will enable the analyses of gene function in diverse insect species. This will greatly increase our understanding of diverse aspects of biology so far not functionally addressable. Moreover, insect transgenesis will provide novel st...

  3. What Makes an Insect an Insect?

    ERIC Educational Resources Information Center

    NatureScope, 1985

    1985-01-01

    Provides background information on characteristics common to all insects, activities, and student materials (ready-to-copy games, puzzles, coloring pages, worksheets, and/or mazes) which describe: how insects are classified; how they are different from other animals; and the main insect characteristics. Activities include recommended age levels,…

  4. The Importance of Change: Contextualizing Natural and Anthropogenic Hydrologic Variability in terms of agricultural and sectorial economies in India

    NASA Astrophysics Data System (ADS)

    Ponce de Leon Barido, D.; Nelson, H.; Thatikonda, S.; Smith, R.; Roe, T.; Foufoula, E.

    2012-12-01

    Freshwater availability and human prosperity are intricately connected. In many parts of the world however, water demands exceed the renewable water supply and groundwater resources are depleted at an alarming rate. Such is the case in India, especially in its agriculturally intensive regions of Punjab and Telagana where most of the country's rice and wheat is produced. Punjab (Northwest India; deep alluvial aquifers), and Telangana (Central India; shallow bedrock aquifers), with vastly different natural resource endowments (water and hydrogeology) and economic structures, present an exemplary case study for depicting the linkages that exist between hydrological variability, climate change adaptation, and regional sectorial economies. We focus our study on precipitation variability and key parameters that can explain how the Indian monsoon has been evolving over time: total and monthly monsoon rainfall, the frequency and length of dry spells, the spatial distribution of rainfall, and the frequency and intensity of extreme events. Using a social accounting matrix (SAM) to describe the sector composition and structure of the Punjab, Telengana and the rest of India, we evaluate the economic implications of variability, adaptation, and policy changes, via a general equilibrium framework. Hydrologic variability and change is given context as "water shocks" are translated to economic consequences allowing to study scenarios and trade-offs.

  5. Insect maintenance and transmission.

    PubMed

    Kingdom, Heather

    2013-01-01

    Phytoplasmas are plant pathogens of huge economic importance due to responsibility for crop yield losses worldwide. Institutions around the world are trying to understand and control this yield loss at a time when food security is high on government agendas. In order to fully understand the mechanisms of phytoplasma infection and spread, more insect vector and phytoplasma colonies will need to be established for research worldwide. Rearing and study of these colonies is essential in the research and development of phytoplasma control measures. This chapter highlights general materials and methods for raising insect vector colonies and maintenance of phytoplasmas. Specific methods of rearing the maize leafhopper and maize bushy stunt phytoplasma and the aster leafhopper and aster yellows phytoplasma strain witches' broom are also included. PMID:22987405

  6. Fungal allelochemicals in insect pest management.

    PubMed

    Holighaus, Gerrit; Rohlfs, Marko

    2016-07-01

    Interactions between insects and fungi are widespread, and important mediators of these interactions are fungal chemicals that can therefore be considered as allelochemicals. Numerous studies suggest that fungal chemicals can affect insects in many different ways. Here, we apply the terminology established by insect-plant ecologists for categorizing the effect of fungal allelochemicals on insects and for evaluating the application potential of these chemicals in insect pest management. Our literature survey shows that fungal volatile and non-volatile chemicals have an enormous potential to influence insect behavior and fitness. Many of them still remain to be discovered, but some recent examples of repellents and toxins could open up new ways for developing safe insect control strategies. However, we also identified shortcomings in our understanding of the chemical ecology of insect-fungus interactions and the way they have been investigated. In particular, the mode-of-action of fungal allelochemicals has often not been appropriately designated or examined, and the way in which induction by insects affects fungal chemical diversity is poorly understood. This review should raise awareness that in-depth ecological studies of insect-fungus interactions can reveal novel allelochemicals of particular benefit for the development of innovative insect pest management strategies. PMID:27147531

  7. Book Review: Insect Virology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Viruses that infect insects have long been of interest both as a means for controlling insect pest populations in an environmentally safe manner, and also as significant threats to beneficial insects of great value, such as honey bees and silkworms. Insect viruses also have been of intrinsic intere...

  8. Insect-ual Pursuits.

    ERIC Educational Resources Information Center

    Mallow, David

    1991-01-01

    Explains how insects can be used to stimulate student writing. Describes how students can create their own systems to classify and differentiate insects. Discusses insect morphology and includes three detailed diagrams. The author provides an extension activity where students hypothesize about the niche of an insect based on its anatomy. (PR)

  9. The relative importance of fertilization and soil erosion on C-dynamics in agricultural landscapes of NE Germany

    NASA Astrophysics Data System (ADS)

    Pohl, Madlen; Hoffmann, Mathias; Hagemann, Ulrike; Jurisch, Nicole; Remus, Rainer; Sommer, Michael; Augustin, Jürgen

    2016-04-01

    The hummocky ground moraine landscape of north-east Germany is characterized by distinct small-scale soil heterogeneity on the one hand, and intensive energy crop cultivation on the other. Both factors are assumed to significantly influence gaseous C exchange, and thus driving the dynamics of soil organic carbon stocks in terrestrial, agricultural ecosystems. However, it is not yet clear to which extent fertilization and soil erosional status influence soil C dynamics and whether one of these factors is more relevant than the other. We present seasonal and dynamic soil C balances of biogas maize for the growing season 2011, recorded at different sites located within the CarboZALF experimental area. The sites differ regarding soils (non-eroded Albic Luvisols (Cutanic), extremely eroded Calcaric Regosol and depositional Endogleyic Colluvic Regosol,) and applied fertilizer (100% mineral N fertilizer, 50% mineral and 50% N organic fertilizer, 100% organic N fertilizer). Fertilization treatments were established on the Albic Luvisol (Cutanic). Net-CO2-exchange (NEE) and ecosystem respiration (Reco) were measured every four weeks using a dynamic flow-through non-steady-state closed manual chamber system. Gap filling was performed based on empirical temperature and PAR dependency functions to derive daily NEE values. At the same time, daily above-ground biomass production (NPP) was estimated based on biomass samples and final harvest, using a sigmoidal growth function. In a next step, dynamic soil C balances were generated as the balance of daily NEE and NPP considering the initial C input due to N fertilizers. The resulted seasonal soil C balances varied from strong C losses at the Endogleyic Colluvic Regosol (602 g C m-2) to C gains at the Calcaric Regosol (-132 g C m-2). In general, soils exerted a stronger impact on seasonal and dynamic C balances compared to differences in applied N fertilizer. There are indications that inter-annual variations in climate conditions

  10. Irradiation of Northwest agricultural products

    SciTech Connect

    Eakin, D.E.; Tingey, G.L.

    1985-02-01

    Irradiation of food for disinfestation and preservation is increasing in importance because of increasing restrictions on various chemical treatments. Irradiation treatment is of particular interest in the Northwest because of a growing supply of agricultural products and the need to develop new export markets. Several products have, or could potentially have, significant export markets if stringent insect control procedures are developed and followed. Due to the recognized potential benefits of irradiation, Pacific Northwest Laboratory (PNL) is conducting this program to evaluate the benefits of using irradiation on Northwest agricultural products under the US Department of Energy (DOE) Defense Byproducts Production and Utilization Program. Commodities currently included in the program are cherries, apples, asparagus, spices, hay, and hides.

  11. Herbivory increases diversification across insect clades

    PubMed Central

    Wiens, John J.; Lapoint, Richard T.; Whiteman, Noah K.

    2015-01-01

    Insects contain more than half of all living species, but the causes of their remarkable diversity remain poorly understood. Many authors have suggested that herbivory has accelerated diversification in many insect clades. However, others have questioned the role of herbivory in insect diversification. Here, we test the relationships between herbivory and insect diversification across multiple scales. We find a strong, positive relationship between herbivory and diversification among insect orders. However, herbivory explains less variation in diversification within some orders (Diptera, Hemiptera) or shows no significant relationship with diversification in others (Coleoptera, Hymenoptera, Orthoptera). Thus, we support the overall importance of herbivory for insect diversification, but also show that its impacts can vary across scales and clades. In summary, our results illuminate the causes of species richness patterns in a group containing most living species, and show the importance of ecological impacts on diversification in explaining the diversity of life. PMID:26399434

  12. Adaptive mechanisms of insect pests against plant protease inhibitors and future prospects related to crop protection: a review.

    PubMed

    Macedo, Maria L R; de Oliveira, Caio F R; Costa, Poliene M; Castelhano, Elaine C; Silva-Filho, Marcio C

    2015-01-01

    The overwhelming demand for food requires the application of technology on field. An important issue that limits the productivity of crops is related to insect attacks. Hence, several studies have evaluated the application of different compounds to reduce the field losses, especially insecticide compounds from plant sources. Among them, plant protease inhibitors (PIs) have been studied in both basic and applied researches, displaying positive results in control of some insects. However, certain species are able to bypass the insecticide effects exerted by PIs. In this review, we disclosed the adaptive mechanisms showed by lepidopteran and coleopteran insects, the most expressive insect orders related to crop predation. The structural aspects involved in adaptation mechanisms are presented as well as the newest alternatives for pest control. The application of biotechnological tools in crop protection will be mandatory in agriculture, and it will be up to researchers to find the best candidates for effective control in long-term. PMID:25329404

  13. 7 CFR 305.40 - Garbage treatment schedules for insect pests and pathogens.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Garbage treatment schedules for insect pests and pathogens. 305.40 Section 305.40 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL... Garbage § 305.40 Garbage treatment schedules for insect pests and pathogens. (a) T415-a, heat...

  14. Interrogating an insect society

    PubMed Central

    Gadagkar, Raghavendra

    2009-01-01

    Insect societies such as those of ants, bees, and wasps consist of 1 or a small number of fertile queens and a large number of sterile or nearly sterile workers. While the queens engage in laying eggs, workers perform all other tasks such as nest building, acquisition and processing of food, and brood care. How do such societies function in a coordinated and efficient manner? What are the rules that individuals follow? How are these rules made and enforced? These questions are of obvious interest to us as fellow social animals but how do we interrogate an insect society and seek answers to these questions? In this article I will describe my research that was designed to seek answers from an insect society to a series of questions of obvious interest to us. I have chosen the Indian paper wasp Ropalidia marginata for this purpose, a species that is abundantly distributed in peninsular India and serves as an excellent model system. An important feature of this species is that queens and workers are morphologically identical and physiologically nearly so. How then does an individual become a queen? How does the queen suppress worker reproduction? How does the queen regulate the nonreproductive activities of the workers? What is the function of aggression shown by different individuals? How and when is the queen's heir decided? I will show how such questions can indeed be investigated and will emphasize the need for a whole range of different techniques of observation and experimentation. PMID:19487678

  15. In silico analysis of the 16S rRNA gene of endophytic bacteria, isolated from the aerial parts and seeds of important agricultural crops.

    PubMed

    Bredow, C; Azevedo, J L; Pamphile, J A; Mangolin, C A; Rhoden, S A

    2015-01-01

    Because of human population growth, increased food production and alternatives to conventional methods of biocontrol and development of plants such as the use of endophytic bacteria and fungi are required. One of the methods used to study microorganism diversity is sequencing of the 16S rRNA gene, which has several advantages, including universality, size, and availability of databases for comparison. The objective of this study was to analyze endophytic bacterial diversity in agricultural crops using published papers, sequence databases, and phylogenetic analysis. Fourteen papers were selected in which the ribosomal 16S rRNA gene was used to identify endophytic bacteria, in important agricultural crops, such as coffee, sugar cane, beans, corn, soybean, tomatoes, and grapes, located in different geographical regions (America, Europe, and Asia). The corresponding 16S rRNA gene sequences were selected from the NCBI database, aligned using the Mega 5.2 program, and phylogenetic analysis was undertaken. The most common orders present in the analyzed cultures were Bacillales, Enterobacteriales, and Actinomycetales and the most frequently observed genera were Bacillus, Pseudomonas, and Microbacterium. Phylogenetic analysis showed that only approximately 1.56% of the total sequences were not properly grouped, demonstrating reliability in the identification of microorganisms. This study identified the main genera found in endophytic bacterial cultures from plants, providing data for future studies on improving plant agriculture, biotechnology, endophytic bacterium prospecting, and to help understand relationships between endophytic bacteria and their interactions with plants. PMID:26345903

  16. Nutrient composition and nutritional importance of green leaves and wild food resources in an agricultural district, Koutiala, in southern Mali.

    PubMed

    Nordeide, M B; Hatløy, A; Følling, M; Lied, E; Oshaug, A

    1996-11-01

    This paper discusses the nutrient composition and the nutritional importance of green leaves and wild gathered foods in an area with surplus food production in Mali. In this West African country, there is little information about the nutrient composition and the nutritional quality of foods in general, and of wild gathered foods in particular. Food frequency was collected in two cross-sectional surveys. Focus group discussions with women in the area were used to collect information about seasonality, availability and preparation of various foods. Selected food samples were collected for chemical analysis of nutrient composition. The food samples of green leaves (Adansonia digitata, Amaranthus viridis, Tamarindus indica, Allium cepa), seeds and flour (Parkia biglobosa) and fruits (Tamarindus indica) were analysed for water, energy, fat, protein, minerals, amino acids and carotenoids. Availability and use of the foods varied with seasons. In the rainy season, wild gathered foods (e.g. A. digitata) were used as much as fresh cultivated foods (e.g., A. viridis and A. cepa). The wild food resources were more frequently used in rural than in urban areas, with A. digitata as the dominating green leaves. Green leaves were rich in energy, protein and minerals (calcium, iron). Leaves of A. viridis were, in particular, rich in beta-carotene (3290 micrograms/100 g). Chemical score in dried green leaves varied from 47 (A. cepa) to 81 (A. digitata), with lysine as the first limiting amino acid. P. biglobosa fermented seeds, with 35% fat and 37% protein were a complementary source of lysine in the diet. Based on the seasonality, the frequency of use and the nutrient contents of selected green leaves and wild gathered foods in Koutiala district, it is concluded that these traditional and locally produced foods are valuable and important nutrient contributors in the diet both in rural and urban areas, but most important in rural areas. PMID:8933199

  17. Gaps in Border Controls Are Related to Quarantine Alien Insect Invasions in Europe

    PubMed Central

    Bacon, Steven James; Bacher, Sven; Aebi, Alexandre

    2012-01-01

    Alien insects are increasingly being dispersed around the world through international trade, causing a multitude of negative environmental impacts and billions of dollars in economic losses annually. Border controls form the last line of defense against invasions, whereby inspectors aim to intercept and stop consignments that are contaminated with harmful alien insects. In Europe, member states depend on one another to prevent insect introductions by operating a first point of entry rule – controlling goods only when they initially enter the continent. However, ensuring consistency between border control points is difficult because there exists no optimal inspection strategy. For the first time, we developed a method to quantify the volume of agricultural trade that should be inspected for quarantine insects at border control points in Europe, based on global agricultural trade of over 100 million distinct origin-commodity-species-destination pathways. This metric was then used to evaluate the performance of existing border controls, as measured by border interception results in Europe between 2003 and 2007. Alarmingly, we found significant gaps between the trade pathways that should be inspected and actual number of interceptions. Moreover, many of the most likely introduction pathways yielded none or very few insect interceptions, because regular interceptions are only made on only a narrow range of pathways. European countries with gaps in border controls have been invaded by higher numbers of quarantine alien insect species, indicating the importance of proper inspections to prevent insect invasions. Equipped with an optimal inspection strategy based on the underlying risks of trade, authorities globally will be able to implement more effective and consistent border controls. PMID:23112835

  18. Molecular characterization of a mutation affecting abscisic acid biosynthesis and consequently stomatal responses to humidity in an agriculturally important species.

    PubMed

    McAdam, Scott A M; Sussmilch, Frances C; Brodribb, Timothy J; Ross, John J

    2015-01-01

    Mutants deficient in the phytohormone abscisic acid (ABA) have been instrumental in determining not only the biosynthetic pathway for this hormone, but also its physiological role in land plants. The wilty mutant of Pisum sativum is one of the classical, well-studied ABA-deficient mutants; however, this mutant remains uncharacterized at a molecular level. Using a candidate gene approach, we show that the wilty mutation affects the xanthoxin dehydrogenase step in ABA biosynthesis. To date, this step has only been represented by mutants in the ABA2 gene of Arabidopsis thaliana. Functional ABA biosynthesis appears to be critical for normal stomatal responses to changes in humidity in angiosperms, with wilty mutant plants having no increase in foliar ABA levels in response to a doubling in vapour pressure deficit, and no closure of stomata. Phylogenetic analysis of the ABA2 gene family from diverse land plants indicates that an ABA-biosynthesis-specific short-chain dehydrogenase (ABA2) evolved in the earliest angiosperms. The relatively recent origin of specificity in this step has important implications for both the evolution of ABA biosynthesis and action in land plants. PMID:26216469

  19. Molecular characterization of a mutation affecting abscisic acid biosynthesis and consequently stomatal responses to humidity in an agriculturally important species

    PubMed Central

    McAdam, Scott A. M.; Sussmilch, Frances C.; Brodribb, Timothy J.; Ross, John J.

    2015-01-01

    Mutants deficient in the phytohormone abscisic acid (ABA) have been instrumental in determining not only the biosynthetic pathway for this hormone, but also its physiological role in land plants. The wilty mutant of Pisum sativum is one of the classical, well-studied ABA-deficient mutants; however, this mutant remains uncharacterized at a molecular level. Using a candidate gene approach, we show that the wilty mutation affects the xanthoxin dehydrogenase step in ABA biosynthesis. To date, this step has only been represented by mutants in the ABA2 gene of Arabidopsis thaliana. Functional ABA biosynthesis appears to be critical for normal stomatal responses to changes in humidity in angiosperms, with wilty mutant plants having no increase in foliar ABA levels in response to a doubling in vapour pressure deficit, and no closure of stomata. Phylogenetic analysis of the ABA2 gene family from diverse land plants indicates that an ABA-biosynthesis-specific short-chain dehydrogenase (ABA2) evolved in the earliest angiosperms. The relatively recent origin of specificity in this step has important implications for both the evolution of ABA biosynthesis and action in land plants. PMID:26216469

  20. Agriculture increases individual fitness.

    PubMed

    Kovaka, Karen; Santana, Carlos; Patel, Raj; Akçay, Erol; Weisberg, Michael

    2016-01-01

    We question the need to explain the onset of agriculture by appealing to the second type of multilevel selection (MLS2). Unlike eusocial insect colonies, human societies do not exhibit key features of evolutionary individuals. If we avoid the mistake of equating Darwinian fitness with health and quality of life, the adoption of agriculture is almost certainly explicable in terms of individual-level selection and individual rationality. PMID:27561384

  1. Selected examples of dispersal of arthropods associated with agricultural crop and animal production

    NASA Technical Reports Server (NTRS)

    Henneberry, T. J.

    1979-01-01

    The economic importance of arthropods in agricultural production systems and the possibilities of using dispersal behavior to develop and manipulate control are examined. Examples of long and short distance dispersal of economic insect pests and beneficial species from cool season host reservoirs and overwintering sites are presented. Significant dispersal of these species often occurring during crop and animal production is discussed.

  2. Evidence for the importance of litter as a co-substrate for MCPA dissipation in an agricultural soil.

    PubMed

    Saleh, Omar; Pagel, Holger; Enowashu, Esther; Devers, Marion; Martin-Laurent, Fabrice; Streck, Thilo; Kandeler, Ellen; Poll, Christian

    2016-03-01

    Environmental controls of 2-methyl-4-chlorophenoxyacetic acid (MCPA) degradation are poorly understood. We investigated whether microbial MCPA degraders are stimulated by (maize) litter and whether this process depends on concentrations of MCPA and litter. In a microcosm experiment, different amounts of litter (0, 10 and 20 g kg(-1)) were added to soils exposed to three levels of the herbicide (0, 5 and 30 mg kg(-1)). The treated soils were incubated at 20 °C for 6 weeks, and samples were taken after 1, 3 and 6 weeks of incubation. In soils with 5 mg kg(-1) MCPA, about 50 % of the MCPA was dissipated within 1 week of the incubation. Almost complete dissipation of the herbicide had occurred by the end of the incubation with no differences between the three litter amendments. At the higher concentration (30 mg kg(-1)), MCPA endured longer in the soil, with only 31 % of the initial amount being removed at the end of the experiment in the absence of litter. Litter addition greatly increased the dissipation rate with 70 and 80 % of the herbicide being dissipated in the 10 and 20 g kg(-1) litter treatments, respectively. Signs of toxic effects of MCPA on soil bacteria were observed from related phospholipid fatty acid (PLFA) analyses, while fungi showed higher tolerance to the increased MCPA levels. The abundance of bacterial tfdA genes in soil increased with the co-occurrence of litter and high MCPA concentration, indicating the importance of substrate availability in fostering MCPA-degrading bacteria and thereby improving the potential for removal of MCPA in the environment. PMID:25943518

  3. Characterization of potent odorants in male giant water bug (Lethocerus indicus Lep. and Serv.), an important edible insect of Southeast Asia.

    PubMed

    Kiatbenjakul, Patthamawadi; Intarapichet, Kanok-Orn; Cadwallader, Keith R

    2015-02-01

    Potent odorants in frozen fresh (FFB) and salted boiled (SBB) male giant water bugs (Lethocerus indicus), or 'Maengdana' in Thai, were characterized by application of direct solvent extraction/solvent-assisted flavour evaporation (SAFE), gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), aroma extract dilution analysis (AEDA) and stable isotope dilution assays (SIDA). Twenty and 27 potent odorants were detected in FFB and SBB, respectively. Most odorants were lipid-derived compounds, including the two most abundant volatile components (E)-2-hexenyl acetate and (E)-2-hexenyl butanoate, which contributed banana-like odours. 2-Acetyl-1-pyrroline and 2-acetyl-2-thiazoline, responsible for popcorn-like odours, were detected in SBB only. An aroma reconstitution model of SBB was constructed in an oil-in-water emulsion matrix using 12 selected potent odorants based on the results of AEDA, accurate compound quantification and the calculated odour-activity values (OAV). Omission studies were carried out to verify the significance of esters, particularly (E)-2-hexenyl acetate was determined to be an important character-impact odorant in male giant water bug aroma. PMID:25172758

  4. Valuing Insect Pollination Services with Cost of Replacement

    PubMed Central

    Allsopp, Mike H.; de Lange, Willem J.; Veldtman, Ruan

    2008-01-01

    Value estimates of ecosystem goods and services are useful to justify the allocation of resources towards conservation, but inconclusive estimates risk unsustainable resource allocations. Here we present replacement costs as a more accurate value estimate of insect pollination as an ecosystem service, although this method could also be applied to other services. The importance of insect pollination to agriculture is unequivocal. However, whether this service is largely provided by wild pollinators (genuine ecosystem service) or managed pollinators (commercial service), and which of these requires immediate action amidst reports of pollinator decline, remains contested. If crop pollination is used to argue for biodiversity conservation, clear distinction should be made between values of managed- and wild pollination services. Current methods either under-estimate or over-estimate the pollination service value, and make use of criticised general insect and managed pollinator dependence factors. We apply the theoretical concept of ascribing a value to a service by calculating the cost to replace it, as a novel way of valuing wild and managed pollination services. Adjusted insect and managed pollinator dependence factors were used to estimate the cost of replacing insect- and managed pollination services for the Western Cape deciduous fruit industry of South Africa. Using pollen dusting and hand pollination as suitable replacements, we value pollination services significantly higher than current market prices for commercial pollination, although lower than traditional proportional estimates. The complexity associated with inclusive value estimation of pollination services required several defendable assumptions, but made estimates more inclusive than previous attempts. Consequently this study provides the basis for continued improvement in context specific pollination service value estimates. PMID:18781196

  5. An Automated Flying-Insect-Detection System

    NASA Technical Reports Server (NTRS)

    Vann, Timi; Andrews, Jane C.; Howell, Dane; Ryan, Robert

    2005-01-01

    An automated flying-insect-detection system (AFIDS) was developed as a proof-of-concept instrument for real-time detection and identification of flying insects. This type of system has use in public health and homeland security decision support, agriculture and military pest management, and/or entomological research. Insects are first lured into the AFIDS integrated sphere by insect attractants. Once inside the sphere, the insect's wing beats cause alterations in light intensity that is detected by a photoelectric sensor. Following detection, the insects are encouraged (with the use of a small fan) to move out of the sphere and into a designated insect trap where they are held for taxonomic identification or serological testing. The acquired electronic wing beat signatures are preprocessed (Fourier transformed) in real-time to display a periodic signal. These signals are sent to the end user where they are graphically displayed. All AFIDS data are pre-processed in the field with the use of a laptop computer equipped with LABVIEW. The AFIDS software can be programmed to run continuously or at specific time intervals when insects are prevalent. A special DC-restored transimpedance amplifier reduces the contributions of low-frequency background light signals, and affords approximately two orders of magnitude greater AC gain than conventional amplifiers. This greatly increases the signal-to-noise ratio and enables the detection of small changes in light intensity. The AFIDS light source consists of high-intensity Al GaInP light-emitting diodes (LEDs). The AFIDS circuitry minimizes brightness fluctuations in the LEDs and when integrated with an integrating sphere, creates a diffuse uniform light field. The insect wing beats isotropically scatter the diffuse light in the sphere and create wing beat signatures that are detected by the sensor. This configuration minimizes variations in signal associated with insect flight orientation.

  6. Symbiont-mediated RNA interference in insects

    PubMed Central

    Whitten, Miranda M. A.; Facey, Paul D.; Del Sol, Ricardo; Fernández-Martínez, Lorena T.; Evans, Meirwyn C.; Mitchell, Jacob J.; Bodger, Owen G.

    2016-01-01

    RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were horizontally transmissible. This represents a significant advance in the ability to deliver RNAi, potentially to a large range of non-model insects. PMID:26911963

  7. Symbiont-mediated RNA interference in insects.

    PubMed

    Whitten, Miranda M A; Facey, Paul D; Del Sol, Ricardo; Fernández-Martínez, Lorena T; Evans, Meirwyn C; Mitchell, Jacob J; Bodger, Owen G; Dyson, Paul J

    2016-02-24

    RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were horizontally transmissible. This represents a significant advance in the ability to deliver RNAi, potentially to a large range of non-model insects. PMID:26911963

  8. Using Insects to Make Healthy Space Foods

    NASA Astrophysics Data System (ADS)

    Katayama, Naomi; Yamashita, Masamichi; Kok, Robert; Space Agriculture Task Force, J.

    Providing foods to space crew is the important requirements to support long term manned space exploration. Foods fill not only physiological requirements to sustain life, but psychological needs for refreshment and joy during the long and hard mission to extraterrestrial planets. We designed joyful and healthy recipe with materials (plants, insects, fish et.cet. la.), which can be produced by the bio-regenerative agricultural system operated at limited resources available in spaceship or on Moon and Mars. And we need to get the storage method of the food without the problem of food poisoning. The consideration about the food allergy is necessary, too. Nutritional analysis on the basic vegetable menu consisting of rice, barley, soybean, sweet potato cassava, quinoa and green reveals a shortage of vitamins D and B12, cholesterol and sodium salt. Since vitamin D deficiency results in demineralization of bone. Vitamin B12 is essential to prevent pernicious anemia. Fish contains both vitamins D and B12. The pupa of the silkworm becomes the important nourishment source as protein and lipid. The silk thread uses it as clothing and cosmetics and medical supplies. However, we can use the silk thread as food as protein. A law of nature shakes high quality oils and fats included in termite for cooking. I use the bee as food after having used it for the pollination of the plant. Of course the honey becomes the important food, too. The snail and mud snail become the food as protein. We decided to use the menu consisting of the basic vegetarian menu plus insect and loach for further conceptual design of space agriculture. We succeeded to develop joyful and nutritious space recipe at the end. Since energy consumption for physical exercise activities under micro-or sub-gravity is less than the terrestrial case, choice of our space foods is essential to suppress blood sugar level, and prevent the metabolic syndrome. Because of less need of agricultural resources at choosing

  9. Insect Bites and Stings

    MedlinePlus

    Most insect bites are harmless, though they sometimes cause discomfort. Bee, wasp, and hornet stings and fire ant bites usually hurt. Mosquito and flea bites usually itch. Insects can also spread diseases. In the United States, ...

  10. Insects: An Interdisciplinary Unit

    ERIC Educational Resources Information Center

    Leger, Heather

    2007-01-01

    The author talks about an interdisciplinary unit on insects, and presents activities that can help students practice communication skills (interpersonal, interpretive, and presentational) and learn about insects with hands-on activities.

  11. Respiration in Aquatic Insects.

    ERIC Educational Resources Information Center

    MacFarland, John

    1985-01-01

    This article: (1) explains the respiratory patterns of several freshwater insects; (2) describes the differences and mechanisms of spiracular cutaneous, and gill respiration; and (3) discusses behavioral aspects of selected aquatic insects. (ML)

  12. Insects and Scorpions

    MedlinePlus

    ... gov . Workplace Safety and Health Topics Insects & Scorpions Bees, Wasps, and Hornets Fire Ants Scorpions Additional Resources ... to outdoor workers. Stinging or biting insects include bees, wasps, hornets, and fire ants. The health effects ...

  13. Ecophysiology and insect herbivory

    SciTech Connect

    Clancy, K.M.; Wagner, M.R.; Reich, P.B.

    1995-07-01

    The relationship of insect herbivory to conifer physiology is examined. Aspects of nutrient assimilation, nutrient distribution, water stress, and climatic change are correlated to defoliation by insects. Other factors examined include plant age, density, structure, soils, and plant genotype.

  14. Insects of war, terror and torture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    From plagues to malaria transmission, insects and other arthropods have been natural or intentional health and agricultural threats to military and civilian populations throughout human history. The success or failure of military operations frequently has been determined by correctly anticipating in...

  15. IRRADIATION FOR POSTHARVEST CONTROL OF QUARANTINE INSECTS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Interest in the use of irradiation as a phytosanitary treatment for agricultural commodities is growing worldwide, particularly since international IPPC and CODEX standards now endorse and facilitate trade based on this disinfestation method. Irradiation is broadly effective against insects and mite...

  16. Acoustic Monitoring of Insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Farmers, grain elevator managers, and food processors often sample grain for insect damaged kernels and numbers of live adult insects but these easily obtained measurements of insect levels do not provide reliable estimates of the typically much larger populations of internally feeding immature inse...

  17. Exploring Sound with Insects

    ERIC Educational Resources Information Center

    Robertson, Laura; Meyer, John R.

    2010-01-01

    Differences in insect morphology and movement during singing provide a fascinating opportunity for students to investigate insects while learning about the characteristics of sound. In the activities described here, students use a free online computer software program to explore the songs of the major singing insects and experiment with making…

  18. Insects and Spiders.

    ERIC Educational Resources Information Center

    National Audubon Society, New York, NY.

    This set of teaching aids consists of nine Audubon Nature Bulletins, providing teachers and students with informational reading on insects and spiders. The bulletins have these titles: What Good Are Insects, How Insects Benefit Man, Life of the Honey Bee, Ants and Their Fascinating Ways, Mosquitoes and Other Flies, Caterpillars, Spiders and Silk,…

  19. Insects and Others.

    ERIC Educational Resources Information Center

    Mills, Richard

    1984-01-01

    Several ideas for observing insects and soil animals in the classroom are provided. Also provided are: (1) procedures for making insect cages with milk cartons; (2) suggestions for collecting and feeding insects; and (3) techniques for collecting and identifying soil animals. (BC)

  20. Interdisciplinary Outdoor Education, Insects.

    ERIC Educational Resources Information Center

    Orsborn, Edward E.

    This manual is a teacher's resource and guide book describing activities for elementary students involving the collecting, killing, preserving, and identification of insects. Most activities relate to collecting and identifying, but activities involving terrariums and hatcheries, finding hidden insects, and insect trapping are also described.…

  1. Sunflower insect pests

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Like other annual crops, sunflowers are fed upon by a variety of insect pests capable of reducing yields. Though there are a few insects which are considered consistent or severe (e.g., sunflower moth, banded sunflower moth, red sunflower seed weevil), many more insects are capable of causing proble...

  2. InsectBase: a resource for insect genomes and transcriptomes.

    PubMed

    Yin, Chuanlin; Shen, Gengyu; Guo, Dianhao; Wang, Shuping; Ma, Xingzhou; Xiao, Huamei; Liu, Jinding; Zhang, Zan; Liu, Ying; Zhang, Yiqun; Yu, Kaixiang; Huang, Shuiqing; Li, Fei

    2016-01-01

    The genomes and transcriptomes of hundreds of insects have been sequenced. However, insect community lacks an integrated, up-to-date collection of insect gene data. Here, we introduce the first release of InsectBase, available online at http://www.insect-genome.com. The database encompasses 138 insect genomes, 116 insect transcriptomes, 61 insect gene sets, 36 gene families of 60 insects, 7544 miRNAs of 69 insects, 96,925 piRNAs of Drosophila melanogaster and Chilo suppressalis, 2439 lncRNA of Nilaparvata lugens, 22,536 pathways of 78 insects, 678,881 untranslated regions (UTR) of 84 insects and 160,905 coding sequences (CDS) of 70 insects. This release contains over 12 million sequences and provides search functionality, a BLAST server, GBrowse, insect pathway construction, a Facebook-like network for the insect community (iFacebook), and phylogenetic analysis of selected genes. PMID:26578584

  3. InsectBase: a resource for insect genomes and transcriptomes

    PubMed Central

    Yin, Chuanlin; Shen, Gengyu; Guo, Dianhao; Wang, Shuping; Ma, Xingzhou; Xiao, Huamei; Liu, Jinding; Zhang, Zan; Liu, Ying; Zhang, Yiqun; Yu, Kaixiang; Huang, Shuiqing; Li, Fei

    2016-01-01

    The genomes and transcriptomes of hundreds of insects have been sequenced. However, insect community lacks an integrated, up-to-date collection of insect gene data. Here, we introduce the first release of InsectBase, available online at http://www.insect-genome.com. The database encompasses 138 insect genomes, 116 insect transcriptomes, 61 insect gene sets, 36 gene families of 60 insects, 7544 miRNAs of 69 insects, 96 925 piRNAs of Drosophila melanogaster and Chilo suppressalis, 2439 lncRNA of Nilaparvata lugens, 22 536 pathways of 78 insects, 678 881 untranslated regions (UTR) of 84 insects and 160 905 coding sequences (CDS) of 70 insects. This release contains over 12 million sequences and provides search functionality, a BLAST server, GBrowse, insect pathway construction, a Facebook-like network for the insect community (iFacebook), and phylogenetic analysis of selected genes. PMID:26578584

  4. Neurosecretion: peptidergic systems in insects

    NASA Astrophysics Data System (ADS)

    Predel, R.; Eckert, Manfred

    Insect neuropeptides are produced in less than 1% of the cells of the central nervous system. Despite this, they are important messenger molecules which influence nearly all physiological processes, including behaviour. They can act as transmitters, modulators and classical hormones, and often exhibit pleiotropic functions when released into the haemolymph. The large number of neuropeptides that has been identified from some of the model organisms among insects underlines the complexity of the neurosecretory system; studies about the coordinated actions of these substances are in their preliminary stages. Recent advances in insect neuropeptide research will be reviewed here, concentrating on the distribution of multiple peptide forms in the central nervous system and adjacent neurohaemal organs, and the role of neuropeptides in eclosion behaviour.

  5. ScaleNet: A literature-based model of scale insect biology and systematics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Scale insects (Hemiptera: Coccoidea) are small herbivorous insects found in all continents except Antarctica. They are extremely invasive, and many species are serious agricultural pests. They are also emerging models for studies of the evolution of genetic systems, endosymbiosis, and plant-insect i...

  6. Ecological Considerations in Producing and Formulating Fungal Entomopathogens for Use in Insect Biocontrol

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insect pests persist in a wide-variety of agricultural, arboreal, and urban environments. Effective control with fungal entomopathogens using inundation biocontrol requires an understanding of the ecology of the target insect, fungal pathogen, and the insect-pathogen interaction. Historically, the d...

  7. Ecological Considerations in Producing and Formulating Fungal Entomopathogens for Use in Insect Biocontrol

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insect pests persist in a wide variety of agricultural, arboreal, and urban environments. Effective control with fungal entomopathogens using inundation biocontrol requires an understanding of the ecology of the target insect, fungal pathogen, and the insect-pathogen interaction. Historically, the...

  8. Identification and Control of Common Insect Pests of Ornamental Shrubs and Trees.

    ERIC Educational Resources Information Center

    Gesell, Stanley G.

    This agriculture extension service publication from Pennsylvania State University introduces the identification and control of common ornamental insect pests. For each of the insects or insect groups (i.e. aphids) identified in this publication, information on host plants, pest description, and damage caused by the pest is given. Also a calendar…

  9. Expression of heat shock protein genes in insect stress responses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The heat shock proteins (HSPs) that are abundantly expressed in insects are important modulators of insect survival. Expression of HSP genes in insects is not only developmentally regulated, but also induced by various stressors in order to confer protection against such stressors. The expression o...

  10. Grain sorghum hybrid resistance to insect and bird damage - 2015

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A total of 26 grain sorghum hybrids (24 commercial grain sorghum hybrids and a pair of sugarcane aphid resistant and susceptible controls) were evaluated for resistance to insect and bird damage in Tifton, Georgia. A total of 10 insect pests were observed. The insect pests in order of importance are...

  11. Grain sorghum hybrid resistance to insect and bird damage-2014

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Thirty seven grain sorghum hybrids were evaluated for resistance to insect and bird damage in 2014 in Tifton, and a total of 10 insect pests were observed. While sorghum midge and bird damage was relatively low, sorghum webworm and aphid damage was high. Those insects in order of importance are: sug...

  12. S-Nitrosoglutathione reductase (GSNOR) mediates the biosynthesis of jasmonic acid and ethylene induced by feeding of the insect herbivore Manduca sexta and is important for jasmonate-elicited responses in Nicotiana attenuata

    PubMed Central

    Wünsche, Hendrik; Baldwin, Ian T.; Wu, Jianqiang

    2011-01-01

    S-nitrosoglutathione reductase (GSNOR) reduces the nitric oxide (NO) adduct S-nitrosoglutathione (GSNO), an essential reservoir for NO bioactivity. In plants, GSNOR has been found to be important in resistance to bacterial and fungal pathogens, but whether it is also involved in plant–herbivore interactions was not known. Using a virus-induced gene silencing (VIGS) system, the activity of GSNOR in a wild tobacco species, Nicotiana attenuata, was knocked down and the function of GSNOR in defence against the insect herbivore Manduca sexta was examined. Silencing GSNOR decreased the herbivory-induced accumulation of jasmonic acid (JA) and ethylene, two important phytohormones regulating plant defence levels, without compromising the activity of two mitogen-activated protein kinases (MAPKs), salicylic acid-induced protein kinase (SIPK) and wound-induced protein kinase (WIPK). Decreased activity of trypsin proteinase inhibitors (TPIs) were detected in GSNOR-silenced plants after simulated M. sexta feeding and bioassays indicated that GSNOR-silenced plants have elevated susceptibility to M. sexta attack. Furthermore, GSNOR is required for methyl jasmonate (MeJA)-induced accumulation of defence-related secondary metabolites (TPI, caffeoylputrescine, and diterpene glycosides) but is not needed for the transcriptional regulation of JAZ3 (jasmonate ZIM-domain 3) and TD (threonine deaminase), indicating that GSNOR mediates certain but not all jasmonate-inducible responses. This work highlights the important role of GSNOR in plant resistance to herbivory and jasmonate signalling and suggests the potential involvement of NO in plant–herbivore interactions. Our data also suggest that GSNOR could be a target of genetic modification for improving crop resistance to herbivores. PMID:21622839

  13. Hydrodynamics of insect spermatozoa

    NASA Astrophysics Data System (ADS)

    Pak, On Shun; Lauga, Eric

    2010-11-01

    Microorganism motility plays important roles in many biological processes including reproduction. Many microorganisms propel themselves by propagating traveling waves along their flagella. Depending on the species, propagation of planar waves (e.g. Ceratium) and helical waves (e.g. Trichomonas) were observed in eukaryotic flagellar motion, and hydrodynamic models for both were proposed in the past. However, the motility of insect spermatozoa remains largely unexplored. An interesting morphological feature of such cells, first observed in Tenebrio molitor and Bacillus rossius, is the double helical deformation pattern along the flagella, which is characterized by the presence of two superimposed helical flagellar waves (one with a large amplitude and low frequency, and the other with a small amplitude and high frequency). Here we present the first hydrodynamic investigation of the locomotion of insect spermatozoa. The swimming kinematics, trajectories and hydrodynamic efficiency of the swimmer are computed based on the prescribed double helical deformation pattern. We then compare our theoretical predictions with experimental measurements, and explore the dependence of the swimming performance on the geometric and dynamical parameters.

  14. Insects Represent a Link between Food Animal Farms and the Urban Environment for Antibiotic Resistance Traits

    PubMed Central

    Ghosh, Anuradha

    2014-01-01

    Antibiotic-resistant bacterial infections result in higher patient mortality rates, prolonged hospitalizations, and increased health care costs. Extensive use of antibiotics as growth promoters in the animal industry represents great pressure for evolution and selection of antibiotic-resistant bacteria on farms. Despite growing evidence showing that antibiotic use and bacterial resistance in food animals correlate with resistance in human pathogens, the proof for direct transmission of antibiotic resistance is difficult to provide. In this review, we make a case that insects commonly associated with food animals likely represent a direct and important link between animal farms and urban communities for antibiotic resistance traits. Houseflies and cockroaches have been shown to carry multidrug-resistant clonal lineages of bacteria identical to those found in animal manure. Furthermore, several studies have demonstrated proliferation of bacteria and horizontal transfer of resistance genes in the insect digestive tract as well as transmission of resistant bacteria by insects to new substrates. We propose that insect management should be an integral part of pre- and postharvest food safety strategies to minimize spread of zoonotic pathogens and antibiotic resistance traits from animal farms. Furthermore, the insect link between the agricultural and urban environment presents an additional argument for adopting prudent use of antibiotics in the food animal industry. PMID:24705326

  15. Spatial decoupling of agricultural production and consumption: quantifying dependences of countries on food imports due to domestic land and water constraints

    NASA Astrophysics Data System (ADS)

    Fader, Marianela; Gerten, Dieter; Krause, Michael; Lucht, Wolfgang; Cramer, Wolfgang

    2013-03-01

    In our globalizing world, the geographical locations of food production and consumption are becoming increasingly disconnected, which increases reliance on external resources and their trade. We quantified to what extent water and land constraints limit countries’ capacities, at present and by 2050, to produce on their own territory the crop products that they currently import from other countries. Scenarios of increased crop productivity and water use, cropland expansion (excluding areas prioritized for other uses) and population change are accounted for. We found that currently 16% of the world population use the opportunities of international trade to cover their demand for agricultural products. Population change may strongly increase the number of people depending on ex situ land and water resources up to about 5.2 billion (51% of world population) in the SRES A2r scenario. International trade will thus have to intensify if population growth is not accompanied by dietary change towards less resource-intensive products, by cropland expansion, or by productivity improvements, mainly in Africa and the Middle East. Up to 1.3 billion people may be at risk of food insecurity in 2050 in present low-income economies (mainly in Africa), if their economic development does not allow them to afford productivity increases, cropland expansion and/or imports from other countries.

  16. Gut immunity in Lepidopteran insects.

    PubMed

    Wu, Kai; Yang, Bing; Huang, Wuren; Dobens, Leonard; Song, Hongsheng; Ling, Erjun

    2016-11-01

    Lepidopteran insects constitute one of the largest fractions of animals on earth, but are considered pests in their relationship with man. Key to the success of this order of insects is its ability to digest food and absorb nutrition, which takes place in the midgut. Because environmental microorganisms can easily enter Lepidopteran guts during feeding, the innate immune response guards against pathogenic bacteria, virus and microsporidia that can be devoured with food. Gut immune responses are complicated by both resident gut microbiota and the surrounding peritrophic membrane and are distinct from immune responses in the body cavity, which depend on the function of the fat body and hemocytes. Due to their relevance to agricultural production, studies of Lepidopteran insect midgut and immunity are receiving more attention, and here we summarize gut structures and functions, and discuss how these confer immunity against different microorganisms. It is expected that increased knowledge of Lepidopteran gut immunity may be utilized for pest biological control in the future. PMID:26872544

  17. Principles of Insect Identification. MP-20.

    ERIC Educational Resources Information Center

    Lawson, Fred A.; Burkhardt, Chris C.

    This document provides information for the complete classification of members of the phylum Arthropoda. Both major and minor insect orders are discussed relative to their anatomical characteristics and importance. (CS)

  18. Qpais: A Web-Based Expert System for Assistedidentification of Quarantine Stored Insect Pests

    NASA Astrophysics Data System (ADS)

    Huang, Han; Rajotte, Edwin G.; Li, Zhihong; Chen, Ke; Zhang, Shengfang

    Stored insect pests can seriously depredate stored products causing worldwide economic losses. Pests enter countries traveling with transported goods. Inspection and Quarantine activities are essential to prevent the invasion and spread of pests. Identification of quarantine stored insect pests is an important component of the China's Inspection and Quarantine procedure, and it is necessary not only to identify whether the species captured is an invasive species, but determine control procedures for stored insect pests. With the development of information technologies, many expert systems that aid in the identification of agricultural pests have been developed. Expert systems for the identification of quarantine stored insect pests are rare and are mainly developed for stand-alone PCs. This paper describes the development of a web-based expert system for identification of quarantine stored insect pests as part of the China 11th Five-Year National Scientific and Technological Support Project (115 Project). Based on user needs, textual knowledge and images were gathered from the literature and expert interviews. ASP.NET, C# and SQL language were used to program the system. Improvement of identification efficiency and flexibility was achieved using a new inference method called characteristic-select-based spatial distance method. The expert system can assist identifying 150 species of quarantine stored insect pests and provide detailed information for each species. The expert system has also been evaluated using two steps: system testing and identification testing. With a 85% rate of correct identification and high efficiency, the system evaluation shows that this expert system can be used in identification work of quarantine stored insect pests.

  19. Identification of olfactory volatiles using gas chromatography-multi-unit recordings (GCMR) in the insect antennal lobe.

    PubMed

    Byers, Kelsey J R P; Sanders, Elischa; Riffell, Jeffrey A

    2013-01-01

    All organisms inhabit a world full of sensory stimuli that determine their behavioral and physiological response to their environment. Olfaction is especially important in insects, which use their olfactory systems to respond to, and discriminate amongst, complex odor stimuli. These odors elicit behaviors that mediate processes such as reproduction and habitat selection(1-3). Additionally, chemical sensing by insects mediates behaviors that are highly significant for agriculture and human health, including pollination(4-6), herbivory of food crops(7), and transmission of disease(8,9). Identification of olfactory signals and their role in insect behavior is thus important for understanding both ecological processes and human food resources and well-being. To date, the identification of volatiles that drive insect behavior has been difficult and often tedious. Current techniques include gas chromatography-coupled electroantennogram recording (GC-EAG), and gas chromatography-coupled single sensillum recordings (GC-SSR)(10-12). These techniques proved to be vital in the identification of bioactive compounds. We have developed a method that uses gas chromatography coupled to multi-channel electrophysiological recordings (termed 'GCMR') from neurons in the antennal lobe (AL; the insect's primary olfactory center)(13,14). This state-of-the-art technique allows us to probe how odor information is represented in the insect brain. Moreover, because neural responses to odors at this level of olfactory processing are highly sensitive owing to the degree of convergence of the antenna's receptor neurons into AL neurons, AL recordings will allow the detection of active constituents of natural odors efficiently and with high sensitivity. Here we describe GCMR and give an example of its use. Several general steps are involved in the detection of bioactive volatiles and insect response. Volatiles first need to be collected from sources of interest (in this example we use flowers

  20. Symbiont-mediated functions in insect hosts

    PubMed Central

    Su, Qi; Zhou, Xiaomao; Zhang, Youjun

    2013-01-01

    The bacterial endosymbionts occur in a diverse array of insect species and are usually rely within the vertical transmission from mothers to offspring. In addition to primary symbionts, plant sap-sucking insects may also harbor several diverse secondary symbionts. Bacterial symbionts play a prominent role in insect nutritional ecology by aiding in digestion of food or supplementing nutrients that insect hosts can’t obtain sufficient amounts from a restricted diet of plant phloem. Currently, several other ecologically relevant traits mediated by endosymbionts are being investigated, including defense toward pathogens and parasites, adaption to environment, influences on insect-plant interactions, and impact of population dynamics. Here, we review recent theoretical predictions and experimental observations of these traits mediated by endosymbionts and suggest that clarifying the roles of symbiotic microbes may be important to offer insights for ameliorating pest invasiveness or impact. PMID:23710278

  1. Oral secretions from Mythimna separata insects specifically induce defence responses in maize as revealed by high-dimensional biological data.

    PubMed

    Qi, Jinfeng; Sun, Guiling; Wang, Lei; Zhao, Chunxia; Hettenhausen, Christian; Schuman, Meredith C; Baldwin, Ian T; Li, Jing; Song, Juan; Liu, Zhudong; Xu, Guowang; Lu, Xin; Wu, Jianqiang

    2016-08-01

    Attack from insect herbivores poses a major threat to plant survival, and accordingly, plants have evolved sophisticated defence systems. Maize is cultivated as a staple crop worldwide, and insect feeding causes large production losses. Despite its importance in agriculture, little is known about how maize reacts to insect herbivory. Taking advantage of advances in sequencing and mass spectrometry technology, we studied the response of maize to mechanical wounding and simulated Mythimna separata (a specialist insect) herbivory by applying its oral secretions (OS) to wounds. In comparison to the responses induced by mechanical wounding, OS elicited larger and longer-lasting changes in the maize transcriptome, proteome, metabolome and phytohormones. Specifically, many genes, proteins and metabolites were uniquely induced or repressed by OS. Nearly 290 transcription factor genes from 39 families were involved in OS-induced responses, and among these, more transcription factor genes were specifically regulated by OS than by wounding. This study provides a large-scale omics dataset for understanding maize response to chewing insects and highlights the essential role of OS in plant-insect interactions. PMID:26991784

  2. From Fossil Parasitoids to Vectors: Insects as Parasites and Hosts.

    PubMed

    Nagler, Christina; Haug, Joachim T

    2015-01-01

    Within Metazoa, it has been proposed that as many as two-thirds of all species are parasitic. This propensity towards parasitism is also reflected within insects, where several lineages independently evolved a parasitic lifestyle. Parasitic behaviour ranges from parasitic habits in the strict sense, but also includes parasitoid, phoretic or kleptoparasitic behaviour. Numerous insects are also the host for other parasitic insects or metazoans. Insects can also serve as vectors for numerous metazoan, protistan, bacterial and viral diseases. The fossil record can report this behaviour with direct (parasite associated with its host) or indirect evidence (insect with parasitic larva, isolated parasitic insect, pathological changes of host). The high abundance of parasitism in the fossil record of insects can reveal important aspects of parasitic lifestyles in various evolutionary lineages. For a comprehensive view on fossil parasitic insects, we discuss here different aspects, including phylogenetic systematics, functional morphology and a direct comparison of fossil and extant species. PMID:26597067

  3. Probing Insect Odorant Receptors with their Cognate Ligands: Insights into Structural Features

    PubMed Central

    Xu, Pingxi; Leal, Walter S.

    2013-01-01

    Oodorant receptors (ORs) are essential for insect survival in the environment and thus are ideal molecular targets for the design of insect-inspired modern green chemicals to control populations of agricultural pests and insects of medical importance. Although insect ORs are known for more than a decade, their structural biology is still in its infancy. Here, we unravel the first structural features of ORs from the malaria mosquito, the Southern house mosquito and the silkworm moth. The second extracellular loops (ECL-2s) of their predicted structures are much longer than ECL-1s and ECL-3s. The 27 amino-acid-residue-long of the ECL-2s in mosquito and the 43 amino-acid-residue-long ECL2s in moth ORs are well-conserved. About one-third of the residues are identical, including 3-4 Pro residues. Thorough examination of well-conserved residues in these structures, by point mutation and functional assay with the Xenopus oocyte recording system, strongly suggest that these “loops” include three β-turns and some degree of folding. In the Southern house mosquito three Pro residues in ECL-2 are essential for full activation of the receptor, which is finely tuned to the oviposition attractant 3-methylindole. Additionally, the “corner residues” of prolines, including Gly, Tyr, and Leu are functionally important thus suggesting that turns are stabilized not only by backbone hydrogen bonds, but also by side-chain interactions. Examination of ECL-2s from a distant taxonomical group suggests these ECL-2 loops might be functionally important in all insect ORs. Two of the four Pro residues in the predicted ECL-2 of the bombykol receptor in the silkworm moth, BmorOR1, are essential for function. Experimental evidence indicates that these loops may not be specificity determinants, but they may form a cover to the yet-to-be-identified membrane embedded binding cavities of insect ORs. PMID:23673297

  4. Insects, infestations and nutrient fluxes

    NASA Astrophysics Data System (ADS)

    Michalzik, B.

    2012-04-01

    Forest ecosystems are characterized by a high temporal and spatial variability in the vertical transfer of energy and matter within the canopy and the soil compartment. The mechanisms and controlling factors behind canopy processes and system-internal transfer dynamics are imperfectly understood at the moment. Seasonal flux diversities and inhomogeneities in throughfall composition have been reported from coniferous and deciduous forests, and in most cases leaf leaching has been considered as principle driver for differences in the amount and quality of nutrients and organic compounds (Tukey and Morgan 1963). Since herbivorous insects and the processes they initiate received less attention in past times, ecologists now emphasize the need for linking biological processes occurring in different ecosystem strata to explain rates and variability of nutrient cycling (Bardgett et al. 1998, Wardle et al. 2004). Consequently, herbivore insects in the canopies of forests are increasingly identified to play an important role for the (re)cycling and availability of nutrients, or, more generally, for the functioning of ecosystems not only in outbreak situations but also at endemic (non-outbreak) density levels (Stadler et al. 2001, Hunter et al. 2003). Before, little attention was paid to insect herbivores when quantifying element and energy fluxes through ecosystems, although the numerous and different functions insects fulfill in ecosystems (e.g. as pollinators, herbivores or detritivores) were unanimously recognized (Schowalter 2000). Amongst the reasons for this restraint was the argument that the total biomass of insects tends to be relatively low compared to the biomass of trees or the pool of soil organic matter (Ohmart et al. 1983). A second argument which was put forward to justify the inferior role of insects in nutrient cycling were the supposed low defoliation losses between 5-10% of the annual leaf biomass, or net primary production, due to insect herbivory under

  5. Diversity in Protein Glycosylation among Insect Species

    PubMed Central

    Vandenborre, Gianni; Smagghe, Guy; Ghesquière, Bart; Menschaert, Gerben; Nagender Rao, Rameshwaram; Gevaert, Kris; Van Damme, Els J. M.

    2011-01-01

    Background A very common protein modification in multicellular organisms is protein glycosylation or the addition of carbohydrate structures to the peptide backbone. Although the Class of the Insecta is the largest animal taxon on Earth, almost all information concerning glycosylation in insects is derived from studies with only one species, namely the fruit fly Drosophila melanogaster. Methodology/Principal Findings In this report, the differences in glycoproteomes between insects belonging to several economically important insect orders were studied. Using GNA (Galanthus nivalis agglutinin) affinity chromatography, different sets of glycoproteins with mannosyl-containing glycan structures were purified from the flour beetle (Tribolium castaneum), the silkworm (Bombyx mori), the honeybee (Apis mellifera), the fruit fly (D. melanogaster) and the pea aphid (Acyrthosiphon pisum). To identify and characterize the purified glycoproteins, LC-MS/MS analysis was performed. For all insect species, it was demonstrated that glycoproteins were related to a broad range of biological processes and molecular functions. Moreover, the majority of glycoproteins retained on the GNA column were unique to one particular insect species and only a few glycoproteins were present in the five different glycoprotein sets. Furthermore, these data support the hypothesis that insect glycoproteins can be decorated with mannosylated O-glycans. Conclusions/Significance The results presented here demonstrate that oligomannose N-glycosylation events are highly specific depending on the insect species. In addition, we also demonstrated that protein O-mannosylation in insect species may occur more frequently than currently believed. PMID:21373189

  6. An Automated Flying-Insect Detection System

    NASA Technical Reports Server (NTRS)

    Vann, Timi; Andrews, Jane C.; Howell, Dane; Ryan, Robert

    2007-01-01

    An automated flying-insect detection system (AFIDS) was developed as a proof-of-concept instrument for real-time detection and identification of flying insects. This type of system has use in public health and homeland-security decision support, agriculture and military pest management, and/or entomological research. Insects are first lured into the AFIDS integrated sphere by insect attractants. Once inside the sphere, the insect s wing beats cause alterations in light intensity that is detected by a photoelectric sensor. Following detection, the insects are encouraged (with the use of a small fan) to move out of the sphere and into a designated insect trap where they are held for taxonomic identification or serological testing. The acquired electronic wing-beat signatures are preprocessed (Fourier transformed) in real time to display a periodic signal. These signals are sent to the end user where they are graphically. All AFIDS data are preprocessed in the field with the use of a laptop computer equipped with LabVIEW. The AFIDS software can be programmed to run continuously or at specific time intervals when insects are prevalent. A special DC-restored transimpedance amplifier reduces the contributions of low-frequency background light signals, and affords approximately two orders of magnitude greater AC gain than conventional amplifiers. This greatly increases the signal-to-noise ratio and enables the detection of small changes in light intensity. The AFIDS light source consists of high-intensity Al-GaInP light-emitting diodes (LEDs). The AFIDS circuitry minimizes brightness fluctuations in the LEDs and when integrated with an integrating sphere, creates a diffuse uniform light field. The insect wing beats isotropically scatter the diffuse light in the sphere and create wing-beat signatures that are detected by the sensor. This configuration minimizes variations in signal associated with insect flight orientation. Preliminary data indicate that AFIDS has

  7. Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusaria.

    PubMed

    O'Donnell, Kerry; Rooney, Alejandro P; Proctor, Robert H; Brown, Daren W; McCormick, Susan P; Ward, Todd J; Frandsen, Rasmus J N; Lysøe, Erik; Rehner, Stephen A; Aoki, Takayuki; Robert, Vincent A R G; Crous, Pedro W; Groenewald, Johannes Z; Kang, Seogchan; Geiser, David M

    2013-03-01

    Fusarium (Hypocreales, Nectriaceae) is one of the most economically important and systematically challenging groups of mycotoxigenic phytopathogens and emergent human pathogens. We conducted maximum likelihood (ML), maximum parsimony (MP) and Bayesian (B) analyses on partial DNA-directed RNA polymerase II largest (RPB1) and second largest subunit (RPB2) nucleotide sequences of 93 fusaria to infer the first comprehensive and well-supported phylogenetic hypothesis of evolutionary relationships within the genus and 20 of its near relatives. Our analyses revealed that Cylindrocarpon formed a basal monophyletic sister to a 'terminal Fusarium clade' (TFC) comprising 20 strongly supported species complexes and nine monotypic lineages, which we provisionally recognize as Fusarium (hypothesis F1). The basal-most divergences within the TFC were only significantly supported by Bayesian posterior probabilities (B-PP 0.99-1). An internode of the remaining TFC, however, was strongly supported by MP and ML bootstrapping and B-PP (hypothesis F2). Analysis of seven Fusarium genome sequences and Southern analysis of fusaria elucidated the distribution of genes required for synthesis of 26 families of secondary metabolites within the phylogenetic framework. Diversification time estimates date the origin of the TFC to the middle Cretaceous 91.3 million years ago. We also dated the origin of several agriculturally important secondary metabolites as well as the lineage responsible for Fusarium head blight of cereals. Dating of several plant-associated species complexes suggests their evolution may have been driven by angiosperm diversification during the Miocene. Our results support two competing hypotheses for the circumscription of Fusarium and provide a framework for future comparative phylogenetic and genomic analyses of this agronomically and medically important genus. PMID:23357352

  8. Crop Farmers' Willingness to Pay for Agricultural Extension Services in Bangladesh: Cases of Selected Villages in Two Important Agro-Ecological Zones

    ERIC Educational Resources Information Center

    Uddin, Ektear MD.; Gao, Qijie; Mamun-Ur-Rashid, MD.

    2016-01-01

    Purpose: Globally, many extension professionals and policy-makers are advocating fee based services, in addressing the fund shortage and sustainable provision of agricultural advisory services. Hence, the article attempts to expose the farmers' willingness to pay (WTP) as agricultural extension in Bangladesh is experiencing chronic fund crisis.…

  9. Assessing insect responses to climate change: What are we testing for? Where should we be heading?

    PubMed

    Andrew, Nigel R; Hill, Sarah J; Binns, Matthew; Bahar, Md Habibullah; Ridley, Emma V; Jung, Myung-Pyo; Fyfe, Chris; Yates, Michelle; Khusro, Mohammad

    2013-01-01

    To understand how researchers are tackling globally important issues, it is crucial to identify whether current research is comprehensive enough to make substantive predictions about general responses. We examined how research on climate change affecting insects is being assessed, what factors are being tested and the localities of studies, from 1703 papers published between 1985 and August 2012. Most published research (64%) is generated from Europe and North America and being dedicated to core data analysis, with 29% of the studies analysed dedicated to Lepidoptera and 22% Diptera: which are well above their contribution to the currently identified insect species richness (estimated at 13% and 17% respectively). Research publications on Coleoptera fall well short of their proportional contribution (19% of publications but 39% of insect species identified), and to a lesser extent so do Hemiptera, and Hymenoptera. Species specific responses to changes in temperature by assessing distribution/range shifts or changes in abundance were the most commonly used methods of assessing the impact of climate change on insects. Research on insects and climate change to date is dominated by manuscripts assessing butterflies in Europe, insects of economic and/or environmental concern in forestry, agriculture, and model organisms. The research on understanding how insects will respond to a rapidly changing climate is still in its infancy, but the current trends of publications give a good basis for how we are attempting to assess insect responses. In particular, there is a crucial need for broader studies of ecological, behavioural, physiological and life history responses to be addressed across a greater range of geographic locations, particularly Asia, Africa and Australasia, and in areas of high human population growth and habitat modification. It is still too early in our understanding of taxa responses to climate change to know if charismatic taxa, such as butterflies, or

  10. Agricultural Plant Pest Control. Manual 93.

    ERIC Educational Resources Information Center

    Missouri Univ., Columbia. Agricultural Experiment Station.

    This training manual provides information needed to meet the minimum EPA standards for certification as a commercial applicator of pesticides for the agricultural plant pest control category. The text discusses the insect pests including caterpillars, beetles, and soil inhabiting insects; diseases and nematodes; and weeds. Consideration is given…

  11. Irrigated agriculture is an important risk factor for West Nile virus disease in the hyperendemic Larimer-Boulder-Weld area of north central Colorado.

    PubMed

    Eisen, Lars; Barker, Christopher M; Moore, Chester G; Pape, W John; Winters, Anna M; Cheronis, Nicholas

    2010-09-01

    This study focused on two West Nile virus (WNV) disease outbreak years, 2003 and 2007, and included a three-county area (Larimer, Boulder, and Weld) in North Central Colorado that is hyperendemic for WNV disease. We used epidemiological data for reported WNV disease cases at the census tract scale to: (1) elucidate whether WNV disease incidence differs between census tracts classified as having high versus lower human population density (based on a threshold value of 580 persons/km2) and (2) determine associations between WNV disease incidence and habitat types suitable as development sites for the larval stage of Culex mosquito vectors. WNV disease incidence was significantly elevated in census tracts with lower human population density, compared with those with high density of human population, in both 2003 (median per census tract of 223 and 143 cases per 100,000 population, respectively) and 2007 (median per census tract of 46 and 19 cases per 100,000 population). This is most likely related, in large part, to greater percentages of coverage in less densely populated census tracts by habitats suitable as development sites for Culex larvae (open water, developed open space, pasture/hay, cultivated crops, woody wetlands, and emergent herbaceous wetlands) and, especially, for the subset of these habitats made up by irrigated agricultural land (pasture/hay and cultivated crops) that presumably serve as major producers of the locally most important vector of WNV to humans: Culex tarsalis. A series of analyses produced significant positive associations between greater coverage of or shorter distance to irrigated agricultural land and elevated WNV disease incidence. As an exercise to produce data with potential to inform spatial implementation schemes for prevention and control measures within the study area, we mapped the spatial patterns, by census tract, of WNV disease incidence in 2003 and 2007 as well as the locations of census tracts that had either low (<25th

  12. Market-based Instruments for optimal control of Invasive Insect Species: B. tabaci in Arizona.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Invasive insect species represent a significant economic risk to both the financial viability of agricultural producers and to the sustainability of U.S. agriculture more generally. With the rapid growth of international trade in agricultural commodities of all types, agricultural systems in the U.S...

  13. Insect prophenoloxidase: the view beyond immunity

    PubMed Central

    Lu, Anrui; Zhang, Qiaoli; Zhang, Jie; Yang, Bing; Wu, Kai; Xie, Wei; Luan, Yun-Xia; Ling, Erjun

    2014-01-01

    Insect prophenoloxidase (PPO) is an important innate immunity protein due to its involvement in cellular and humoral defense. It belongs to a group of type-3 copper-containing proteins that occurs in almost all organisms. Insect PPO has been studied for over a century, and the PPO activation cascade is becoming clearer. The insect PPO activation pathway incorporates several important proteins, including pattern-recognition receptors (PGRP, β GRP, and C-type lectins), serine proteases, and serine protease inhibitors (serpins). Due to their complexity, PPO activation mechanisms vary among insect species. Activated phenoloxidase (PO) oxidizes phenolic molecules to produce melanin around invading pathogens and wounds. The crystal structure of Manduca sexta PPO shows that a conserved amino acid, phenylalanine (F), can block the active site pocket. During activation, this blocker must be dislodged or even cleaved at the N-terminal sequence to expose the active site pockets and allow substrates to enter. Thanks to the crystal structure of M. sexta PPO, some domains and specific amino acids that affect PPO activities have been identified. Further studies of the relationship between PPO structure and enzyme activities will provide an opportunity to examine other type-3 copper proteins, and trace when and why their various physiological functions evolved. Recent researches show that insect PPO has a relationship with neuron activity, longevity, feces melanization (phytophagous insects) and development, which suggests that it is time for us to look back on insect PPO beyond the view of immunity in this review. PMID:25071597

  14. Climate Change and Tritrophic Interactions: Will Modifications to Greenhouse Gas Emissions Increase the Vulnerability of Herbivorous Insects to Natural Enemies?

    PubMed

    Boullis, Antoine; Francis, Frederic; Verheggen, François J

    2015-04-01

    Insects are highly dependent on odor cues released into the environment to locate conspecifics or food sources. This mechanism is particularly important for insect predators that rely on kairomones released by their prey to detect them. In the context of climate change and, more specifically, modifications in the gas composition of the atmosphere, chemical communication-mediating interactions between phytophagous insect pests, their host plants, and their natural enemies is likely to be impacted. Several reports have indicated that modifications to plants caused by elevated carbon dioxide and ozone concentrations might indirectly affect insect herbivores, with community-level modifications to this group potentially having an indirect influence on higher trophic levels. The vulnerability of agricultural insect pests toward their natural enemies under elevated greenhouse gases concentrations has been frequently reported, but conflicting results have been obtained. This literature review shows that the higher levels of carbon dioxide, as predicted for the coming century, do not enhance the abundance or efficiency of natural enemies to locate hosts or prey in most published studies. Increased ozone levels lead to modifications in herbivore-induced volatile organic compounds (VOCs) released by damaged plants, which may impact the attractiveness of these herbivores to the third trophic level. Furthermore, other oxidative gases (such as SO2 and NO2) tend to reduce the abundance of natural enemies. The impact of changes in atmospheric gas emissions on plant-insect and insect-insect chemical communication has been under-documented, despite the significance of these mechanisms in tritrophic interactions. We conclude by suggesting some further prospects on this topic of research yet to be investigated. PMID:26313181

  15. Corazonin in insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Corazonin is a peptidergic neurohormone of insects which is expressed in neurosecretory neurons of the pars lateralis of the protocerebrum and transported via nervi corpus cardiaci in the storage lobes of the corpora cardiaca. This peptide occurs with a single isofomr in all insects studied so far,...

  16. Insects: Bugged Out!

    ERIC Educational Resources Information Center

    Piehl, Kathy

    2011-01-01

    Insects really need no introduction. They have lived on earth much longer than humans and vastly outnumber people and all other animal species combined. People encounter them daily in their houses and yards. Yet, when children want to investigate insects, books can help them start their explorations. "Paleo Bugs" carries readers back to the time…

  17. Insects and Bugs

    ERIC Educational Resources Information Center

    Sutherland, Karen

    2009-01-01

    They have been around for centuries. They sting, they bite. They cause intense itching or painful sores. They even cause allergic reactions and sometimes death. There are two types of insects that are pests to humans--those that sting and those that bite. The insects that bite do so with their mouths and include mosquitoes, chiggers, and ticks.…

  18. Effects on Insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effects of controlled and modified atmospheres on insects is reviewed and summarized in this chapter. Traditionally, controlled and modified atmospheres are used to store and preserve fresh fruits and vegetables. The effects on insects and the potential of these treatments are secondary to the...

  19. Principal Areas of Insect Research

    ERIC Educational Resources Information Center

    Williams, Carroll M.

    1973-01-01

    Research for insect control has been quite complex. However, recent knowledge of using insect hormones against them has opened new vistas for producing insecticides which may be harmless to human population. Current areas of insect research are outlined. (PS)

  20. Mycetocyte symbiosis in insects.

    PubMed

    Douglas, A E

    1989-11-01

    1. Non-pathogenic microorganisms, known as mycetocyte symbionts, are located in specialized 'mycetocyte' cells of many insects that feed on nutritionally unbalanced or poor diets. The insects include cockroaches, Cimicidae and Lygaeidae (Heteroptera), the Homoptera, Anoplura, the Diptera Pupiparia, some formicine ants and many beetles. 2. Most mycetocyte symbionts are prokaryotes and a great diversity of forms has been described. None has been cultured in vitro and their taxonomic position is obscure. Yeasts have been reported in Cerambycidae and Anobiidae (Coleoptera) and a few planthoppers. They are culturable and those in anobiids have been assigned to the genus Torulopsis. 3. The mycetocyte cells may be associated with the gut, lie free in the abdominal haemocoel or be embedded in the fat body of the insect. The mycetocytes are large polyploid cells which rarely divide and the symbionts are restricted to their cytoplasm. 4. The mycetocyte symbionts are transmitted maternally from one insect generation to the next. In many beetles (Anobiidae, Cerambycidae, Chrysomelidae and cleonine Curculionidae), the microoganisms are smeared onto the eggs and consumed by the hatching larvae. In other insects, they are transferred from mycetocytes to oocytes in the ovary, a process known as transovarial transmission. The details of transmission in the different insect groups vary with the age of the mother (adult, larva or embryo) at which symbiont transfer to the ovary is initiated; whether isolated symbionts or intact mycetocytes are transferred; and the site of entry of symbionts to the egg (anterior, posterior or apolar). 5. Within an individual insect, the biomass of symbionts varies in a regular fashion with age, weight and sex of the insect. Suppression of symbiont growth rate and lysis of 'excess' microorganisms may contribute to the regulation of symbionts (including freshly-isolated preparations of unculturable forms) are used to investigate interactions between the

  1. Agriculture Education. Agriculture Structures.

    ERIC Educational Resources Information Center

    Stuttgart Public Schools, AR.

    This curriculum guide is designed for group instruction of secondary agricultural education students enrolled in one or two semester-long courses in agriculture structures. The guide presents units of study in the following areas: (1) shop safety, (2) identification and general use of hand tools, (3) power tools, (4) carpentry, (5) blueprint…

  2. Emerging strategies for RNA interference (RNAi) applications in insects

    PubMed Central

    Nandety, Raja Sekhar; Kuo, Yen-Wen; Nouri, Shahideh; Falk, Bryce W

    2015-01-01

    RNA interference (RNAi) in insects is a gene regulatory process that also plays a vital role in the maintenance and in the regulation of host defenses against invading viruses. Small RNAs determine the specificity of the RNAi through precise recognition of their targets. These small RNAs in insects comprise small interfering RNAs (siRNAs), micro RNAs (miRNAs) and Piwi interacting RNAs (piRNAs) of various lengths. In this review, we have explored different forms of the RNAi inducers that are presently in use, and their applications for an effective and efficient fundamental and practical RNAi research with insects. Further, we reviewed trends in next generation sequencing (NGS) technologies and their importance for insect RNAi, including the identification of novel insect targets as well as insect viruses. Here we also describe a rapidly emerging trend of using plant viruses to deliver the RNAi inducer molecules into insects for an efficient RNAi response. PMID:25424593

  3. Prostaglandins and Their Receptors in Insect Biology

    PubMed Central

    Stanley, David; Kim, Yonggyun

    2011-01-01

    We treat the biological significance of prostaglandins (PGs) and their known receptors in insect biology. PGs and related eicosanoids are oxygenated derivatives of arachidonic acid (AA) and two other C20 polyunsaturated fatty acids. PGs are mostly appreciated in the context of biomedicine, but a growing body of literature indicates the biological significance of these compounds extends throughout the animal kingdom, and possibly beyond. The actions of most PGs are mediated by specific receptors. Biomedical research has discovered a great deal of knowledge about PG receptors in mammals, including their structures, pharmacology, molecular biology and cellular locations. Studies of PG receptors in insects lag behind the biomedical background, however, recent results hold the promise of accelerated research in this area. A PG receptor has been identified in a class of lepidopteran hemocytes and experimentally linked to the release of prophenoloxidase. PGs act in several crucial areas of insect biology. In reproduction, a specific PG, PGE2, releases oviposition behavior in most crickets and a few other insect species; PGs also mediate events in egg development in some species, which may represent all insects. PGs play major roles in modulating fluid secretion in Malpighian tubules, rectum and salivary glands, although, again, this has been studied in only a few insect species that may represent the Class. Insect immunity is a very complex defense system. PGs and other eicosanoids mediate a large number of immune reactions to infection and invasion. We conclude that research into PGs and their receptors in insects will lead to important advances in our understanding of insect biology. PMID:22654840

  4. Endosymbiotic bacteria in insects: guardians of the immune system?

    PubMed Central

    Eleftherianos, Ioannis; Atri, Jaishri; Accetta, Julia; Castillo, Julio C.

    2013-01-01

    Insects have evolved obligate, mutualistic interactions with bacteria without further transmission to other eukaryotic organisms. Such long-term obligate partnerships between insects and bacteria have a profound effect on various physiological functions of the host. Here we provide an overview of the effects of endosymbiotic bacteria on the insect immune system as well as on the immune response of insects to pathogenic infections. Potential mechanisms through which endosymbionts can affect the ability of their host to resist an infection are discussed in the light of recent findings. We finally point out unresolved questions for future research and speculate how the current knowledge can be employed to design and implement measures for the effective control of agricultural insect pests and vectors of diseases. PMID:23508299

  5. Microbiome influences on insect host vector competence

    PubMed Central

    Weiss, Brian

    2011-01-01

    Insect symbioses lack the complexity and diversity of those associated with higher eukaryotic hosts. Symbiotic microbiomes are beneficial to their insect hosts in many ways, including dietary supplementation, tolerance to environmental perturbations and maintenance and/or enhancement of host immune system homeostasis. Recent studies have also highlighted the importance of the microbiome in the context of host pathogen transmission processes. Here we provide an overview of the relationship between insect disease vectors, such as tsetse flies and mosquitoes, and their associated microbiome. Several mechanisms are discussed through which symbiotic microbes may influence their host’s ability to transmit pathogens, as well as potential disease control strategies that harness symbiotic microbes to reduce pathogen transmission through an insect vector. PMID:21697014

  6. A systematic nomenclature for the insect brain.

    PubMed

    Ito, Kei; Shinomiya, Kazunori; Ito, Masayoshi; Armstrong, J Douglas; Boyan, George; Hartenstein, Volker; Harzsch, Steffen; Heisenberg, Martin; Homberg, Uwe; Jenett, Arnim; Keshishian, Haig; Restifo, Linda L; Rössler, Wolfgang; Simpson, Julie H; Strausfeld, Nicholas J; Strauss, Roland; Vosshall, Leslie B

    2014-02-19

    Despite the importance of the insect nervous system for functional and developmental neuroscience, descriptions of insect brains have suffered from a lack of uniform nomenclature. Ambiguous definitions of brain regions and fiber bundles have contributed to the variation of names used to describe the same structure. The lack of clearly determined neuropil boundaries has made it difficult to document precise locations of neuronal projections for connectomics study. To address such issues, a consortium of neurobiologists studying arthropod brains, the Insect Brain Name Working Group, has established the present hierarchical nomenclature system, using the brain of Drosophila melanogaster as the reference framework, while taking the brains of other taxa into careful consideration for maximum consistency and expandability. The following summarizes the consortium's nomenclature system and highlights examples of existing ambiguities and remedies for them. This nomenclature is intended to serve as a standard of reference for the study of the brain of Drosophila and other insects. PMID:24559671

  7. Purification and characterization of recombinant sugarcane sucrose phosphate synthase expressed in E. coli and insect Sf9 cells: an importance of the N-terminal domain for an allosteric regulatory property.

    PubMed

    Sawitri, Widhi Dyah; Narita, Hirotaka; Ishizaka-Ikeda, Etsuko; Sugiharto, Bambang; Hase, Toshiharu; Nakagawa, Atsushi

    2016-06-01

    Sucrose phosphate synthase (SPS) catalyses the transfer of glycosyl group of uridine diphosphate glucose to fructose-6-phosphate to form sucrose-6-phosphate. Plant SPS plays a key role in photosynthetic carbon metabolisms, which activity is modulated by an allosteric activator glucose-6-phosphate (G6P). We produced recombinant sugarcane SPS using Escherichia coli and Sf9 insect cells to investigate its structure-function relationship. When expressed in E. coli, two forms of SPS with different sizes appeared; the larger was comparable in size with the authentic plant enzyme and the shorter was trimmed the N-terminal 20 kDa region off. In the insect cells, only enzyme with the authentic size was produced. We purified the trimmed SPS and the full size enzyme from insect cells and found their enzymatic properties differed significantly; the full size enzyme was activated allosterically by G6P, while the trimmed one showed a high activity even without G6P. We further introduced a series of N-terminal truncations up to 171 residue and found G6P-independent activity was enhanced by the truncation. These combined results indicated that the N-terminal region of sugarcane SPS is crucial for the allosteric regulation by G6P and may function like a suppressor domain for the enzyme activity. PMID:26826371

  8. Advanced technologies for genetically manipulating the silkworm Bombyx mori, a model Lepidopteran insect

    PubMed Central

    Xu, Hanfu; O'Brochta, David A.

    2015-01-01

    Genetic technologies based on transposon-mediated transgenesis along with several recently developed genome-editing technologies have become the preferred methods of choice for genetically manipulating many organisms. The silkworm, Bombyx mori, is a Lepidopteran insect of great economic importance because of its use in silk production and because it is a valuable model insect that has greatly enhanced our understanding of the biology of insects, including many agricultural pests. In the past 10 years, great advances have been achieved in the development of genetic technologies in B. mori, including transposon-based technologies that rely on piggyBac-mediated transgenesis and genome-editing technologies that rely on protein- or RNA-guided modification of chromosomes. The successful development and application of these technologies has not only facilitated a better understanding of B. mori and its use as a silk production system, but also provided valuable experiences that have contributed to the development of similar technologies in non-model insects. This review summarizes the technologies currently available for use in B. mori, their application to the study of gene function and their use in genetically modifying B. mori for biotechnology applications. The challenges, solutions and future prospects associated with the development and application of genetic technologies in B. mori are also discussed. PMID:26108630

  9. Transposon-free insertions for insect genetic engineering.

    PubMed

    Dafa'alla, Tarig H; Condon, George C; Condon, Kirsty C; Phillips, Caroline E; Morrison, Neil I; Jin, Li; Epton, Matthew J; Fu, Guoliang; Alphey, Luke

    2006-07-01

    Methods involving the release of transgenic insects in the field hold great promise for controlling vector-borne diseases and agricultural pests. Insect transformation depends on nonautonomous transposable elements as gene vectors. The resulting insertions are stable in the absence of suitable transposase, however, such absence cannot always be guaranteed. We describe a method for post-integration elimination of all transposon sequences in the pest insect Medfly, Ceratitis capitata. The resulting insertions lack transposon sequences and are therefore impervious to transposase activity. PMID:16823373

  10. U.S. Farm and Farm-Related Employment in 1988. How Large, Important, and Regionally Different? Agriculture Information Bulletin Number 634.

    ERIC Educational Resources Information Center

    Majchrowicz, T. Alexander; Hopkins, David E.

    Farm and farm-related industries account for almost 18 percent of total U.S. employment in 1988. This share is based on a broad definition of the agricultural sector, including not only farm production but also industries that mine, manufacture, and sell farm inputs; process commodities; and sell consumer goods. Many jobs in industries that…

  11. Investigation on penetration of three conventional foodstuffs packaging polymers with two different thicknesses by larvae and adults of major species of stored-product pest insects.

    PubMed

    Allahvaisi, Somayeh; Purmirza, Ali Asghar; Safaralizade, Mohamad Hasan

    2009-01-01

    Despite modern methods of packaging, stored agricultural products are still under attack by stored-insect pests. Therefore, determination of the best polymer and appropriate thickness inhibiting the penetration of the insects must be considered. In this study, we investigated the ability of penetration and the rates of contamination by nine important stored product pest insects for three conventional flexible polymers (polyethylene, cellophane and polypropylene) at two thicknesses (16.5 and 29 microm), which are used as pouches for packing of agricultural products. We used adults of T. castaneum (Coleoptera), S. granarius (Coleoptera), R. dominica (Coleoptera), C. maculates (Coleoptera), O. surinamensis (Coleoptera), and larvae of P. interpunctella (Lepidoptera), E. kuehniella (Lepidoptera), S. cerealella (Lepidoptera) and T. granarium (Coleoptera). Results showed that for most of the species penetration occurred between 4 days and 2 weeks, but there were significant differences (p < or = 0.05) in the penetration of three polymers (cellophane, polyethylene and polypropylene) by the insects. Among the polymers, polyethylene with a thickness of 16.5 microm showed the highest degree of penetration and was the most unsuitable polymer for packaging of foodstuffs. Application of this polymer led to a complete infestation of the product and a lot of punctures were created by the insects. In contrast, no penetration was observed in polypropylene polymer with a thickness of 29 microm. Furthermore, adults and larvae of all species showed a much lower penetration when there was no food present in the pouches and this was the case for all polymers tested. PMID:20222605

  12. Range expansion of the invasive insect Greenidea (Trichosiphon) psidii (Hemiptera: Aphididae) in the Neotropical Region.

    PubMed

    Culik, M P; Ventura, J A; Dos S Martins, D

    2016-01-01

    Greenidea psidii is an invasive insect from Asia that feeds on a diverse variety of agriculturally and environmentally important plant species. As an essential component of research necessary for development of a better understanding of biodiversity and its conservation, this study documents a major recent expansion in range of G. psidii in the Neotropics to the region of the tropical restinga ecosystem of Brazil, where it was found infesting guava (Psidium guajava) and jabuticaba (Plinia cauliflora). A summary of information on the geographic distribution, host plants, identification, and potential natural enemies of G. psidii that may be useful for integrated management of this pest in the Neotropical Region and other areas where this invasive insect has recently become established and is likely to further spread is also provided. PMID:27376002

  13. Agricultural Science Protects Our Environment.

    ERIC Educational Resources Information Center

    1967

    Included are a 49 frame filmstrip and a script for narrating a presentation. The presentation is aimed at the secondary school level with an emphasis on how agricultural scientists investigate problems in farmland erosion, stream pollution, road building erosion problems, air pollution, farm pollution, pesticides, and insect control by biological…

  14. Insects and climate change

    SciTech Connect

    Elias, S.A. )

    1991-09-01

    In this article the author describes some of the significant late glacial and Holocene changes that occurred in the Rocky Mountains, including the regional extirpation of certain beetle species. The fossil data presented here summarize what is known about regional insect responses to climate change in terms of species stability and geographic distribution. To minimize potential problems of species interactions (i.e., insect-host plant relationships, host-parasite relationships, and other interactions that tie a particular insect species' distribution to that of another organism), only predators and scavengers are discussed. These insects respond most rapidly to environmental changes, because for the most part they are not tied to any particular type of vegetation.

  15. Insect hemolymph clotting.

    PubMed

    Dushay, Mitchell S

    2009-08-01

    The clot's appearance in different large-bodied insects has been described, but until recently, little was known about any insect clot's molecular makeup, and few experiments could directly test its function. Techniques have been developed in Drosophila (fruit fly) larvae to identify clotting factors that can then be tested for effects on hemostasis, healing, and immunity. This has revealed unanticipated complexity in the hemostatic mechanisms in these larvae. While the clot's molecular structure is not yet fully understood, progress is being made, and the loss of clotting factors has been shown to cause subtle immune defects. The few similarities between coagulation in different insect species and life stages, and the current state of knowledge about coagulation in insects are discussed. PMID:19418022

  16. Evolution of the Insects

    NASA Astrophysics Data System (ADS)

    Grimaldi, David; Engel, Michael S.

    2005-05-01

    This book chronicles the complete evolutionary history of insects--their living diversity and relationships as well as 400 million years of fossils. Introductory sections cover the living species diversity of insects, methods of reconstructing evolutionary relationships, basic insect structure, and the diverse modes of insect fossilization and major fossil deposits. Major sections then explore the relationships and evolution of each order of hexapods. The volume also chronicles major episodes in the evolutionary history of insects from their modest beginnings in the Devonian and the origin of wings hundreds of millions of years before pterosaurs and birds to the impact of mass extinctions and the explosive radiation of angiosperms on insects, and how they evolved into the most complex societies in nature. Whereas other volumes focus on either living species or fossils, this is the first comprehensive synthesis of all aspects of insect evolution. Illustrated with 955 photo- and electron- micrographs, drawings, diagrams, and field photos, many in full color and virtually all of them original, this reference will appeal to anyone engaged with insect diversity--professional entomologists and students, insect and fossil collectors, and naturalists. David Grimaldi and Michael S. Engel have collectively published over 200 scientific articles and monographs on the relationships and fossil record of insects, including 10 articles in the journals Science, Nature, and Proceedings of the National Academy of Sciences. David Grimaldi is curator in the Division of Invertebrate Zoology, American Museum of Natural History and adjunct professor at Cornell University, Columbia University, and the City University of New York. David Grimaldi has traveled in 40 countries on 6 continents, collecting and studying recent species of insects and conducting fossil excavations. He is the author of Amber: Window to the Past (Abrams, 2003). Michael S. Engel is an assistant professor in the

  17. Exploring Insect Vision

    ERIC Educational Resources Information Center

    Damonte, Kathleen

    2005-01-01

    A fly is buzzing around in the kitchen. You sneak up on it with a flyswatter, but just as you get close to it, it flies away. What makes flies and other insects so good at escaping from danger? The fact that insects have eyesight that can easily detect moving objects is one of the things that help them survive. In this month's Science Shorts,…

  18. Biotechnology and Agriculture.

    ERIC Educational Resources Information Center

    Kenney, Martin

    Even at this early date in the application of biotechnology to agriculture, it is clear that agriculture may provide the largest market for new or less expensive biotechnologically manufactured products. The chemical and pharmaceutical industries that hold important positions in agricultural inputs are consolidating their positions by purchasing…

  19. Insect--plant adaptations.

    PubMed

    Southwood, T R

    1984-01-01

    The adaptation of insects to plants probably commenced in the early Permian period, though most current associations will be more recent. A major burst of adaptation must have followed the rise of the Angiosperms in the Cretaceous period, though some particular associations are as recent as this century. Living plants form a large proportion of the potential food in most habitats, though insects have had to overcome certain general hurdles to live and feed on them. Insects affect the reproduction and survival of plants, and thus the diversity of plant secondary chemicals may have evolved as a response. Where an insect species has a significant effect on a plant species that is its only host, coevolution may be envisaged. A spectacular example is provided by Heliconius butterflies and passion flower vines, studied by L.E. Gilbert and others. But such cases may be likened to 'vortices in the evolutionary stream': most plant species are influenced by a range of phytophagous insects so that selection will be for general defences--a situation termed diffuse coevolution. Evidence is presented on recent host-plant shifts to illustrate both the restrictions and the flexibility in current insect-plant associations. PMID:6559112

  20. Insect immunology and hematopoiesis.

    PubMed

    Hillyer, Julián F

    2016-05-01

    Insects combat infection by mounting powerful immune responses that are mediated by hemocytes, the fat body, the midgut, the salivary glands and other tissues. Foreign organisms that have entered the body of an insect are recognized by the immune system when pathogen-associated molecular patterns bind host-derived pattern recognition receptors. This, in turn, activates immune signaling pathways that amplify the immune response, induce the production of factors with antimicrobial activity, and activate effector pathways. Among the immune signaling pathways are the Toll, Imd, Jak/Stat, JNK, and insulin pathways. Activation of these and other pathways leads to pathogen killing via phagocytosis, melanization, cellular encapsulation, nodulation, lysis, RNAi-mediated virus destruction, autophagy and apoptosis. This review details these and other aspects of immunity in insects, and discusses how the immune and circulatory systems have co-adapted to combat infection, how hemocyte replication and differentiation takes place (hematopoiesis), how an infection prepares an insect for a subsequent infection (immune priming), how environmental factors such as temperature and the age of the insect impact the immune response, and how social immunity protects entire groups. Finally, this review highlights some underexplored areas in the field of insect immunobiology. PMID:26695127

  1. Commercial Pesticides Applicator Manual: Agriculture - Plant.

    ERIC Educational Resources Information Center

    Fitzwater, W. D.; And Others

    This training manual provides information needed to meet the minimum EPA standards for certification as a commercial applicator of pesticides in the agriculture-plant pest control category. The text discusses identification and control of insects, diseases, nematodes, and weeds of agricultural crops. Proper use of application equipment and safety…

  2. Transmission and retention of Salmonella enterica by phytophagous hemipteran insects.

    PubMed

    Soto-Arias, José Pablo; Groves, Russell L; Barak, Jeri D

    2014-09-01

    Several pest insects of human and livestock habitations are known as vectors of Salmonella enterica; however, the role of plant-feeding insects as vectors of S. enterica to agricultural crops remains unexamined. Using a hemipteran insect pest-lettuce system, we investigated the potential for transmission and retention of S. enterica. Specifically, Macrosteles quadrilineatus and Myzus persicae insects were fed S. enterica-inoculated lettuce leaf discs or artificial liquid diets confined in Parafilm sachets to allow physical contact or exclusively oral ingestion of the pathogen, respectively. After a 24-h acquisition access period, insects were moved onto two consecutive noninoculated leaf discs or liquid diets and allowed a 24-h inoculation access period on each of the two discs or sachets. Similar proportions of individuals from both species ingested S. enterica after a 24-h acquisition access period from inoculated leaf discs, but a significantly higher proportion of M. quadrilineatus retained the pathogen internally after a 48-h inoculation access period. S. enterica was also recovered from the honeydew of both species. After a 48-h inoculation access period, bacteria were recovered from a significantly higher proportion of honeydew samples from M. quadrilineatus than from M. persicae insects. The recovery of S. enterica from leaf discs and liquid diets postfeeding demonstrated that both species of insects were capable of transmitting the bacteria in ways that are not limited to mechanical transmission. Overall, these results suggest that phytophagous insects may serve as potential vectors of S. enterica in association with plants. PMID:24973069

  3. Transmission and Retention of Salmonella enterica by Phytophagous Hemipteran Insects

    PubMed Central

    Soto-Arias, José Pablo; Groves, Russell L.

    2014-01-01

    Several pest insects of human and livestock habitations are known as vectors of Salmonella enterica; however, the role of plant-feeding insects as vectors of S. enterica to agricultural crops remains unexamined. Using a hemipteran insect pest-lettuce system, we investigated the potential for transmission and retention of S. enterica. Specifically, Macrosteles quadrilineatus and Myzus persicae insects were fed S. enterica-inoculated lettuce leaf discs or artificial liquid diets confined in Parafilm sachets to allow physical contact or exclusively oral ingestion of the pathogen, respectively. After a 24-h acquisition access period, insects were moved onto two consecutive noninoculated leaf discs or liquid diets and allowed a 24-h inoculation access period on each of the two discs or sachets. Similar proportions of individuals from both species ingested S. enterica after a 24-h acquisition access period from inoculated leaf discs, but a significantly higher proportion of M. quadrilineatus retained the pathogen internally after a 48-h inoculation access period. S. enterica was also recovered from the honeydew of both species. After a 48-h inoculation access period, bacteria were recovered from a significantly higher proportion of honeydew samples from M. quadrilineatus than from M. persicae insects. The recovery of S. enterica from leaf discs and liquid diets postfeeding demonstrated that both species of insects were capable of transmitting the bacteria in ways that are not limited to mechanical transmission. Overall, these results suggest that phytophagous insects may serve as potential vectors of S. enterica in association with plants. PMID:24973069

  4. Insect Pathogenic Fungi as Endophytes.

    PubMed

    Moonjely, S; Barelli, L; Bidochka, M J

    2016-01-01

    In this chapter, we explore some of the evolutionary, ecological, molecular genetics, and applied aspects of a subset of insect pathogenic fungi that also have a lifestyle as endophytes and we term endophytic insect pathogenic fungi (EIPF). We focus particularly on Metarhizium spp. and Beauveria bassiana as EIPF. The discussion of the evolution of EIPF challenges a view that these fungi were first and foremost insect pathogens that eventually evolved to colonize plants. Phylogenetic evidence shows that the lineages of EIPF are most closely related to grass endophytes that diverged c. 100MYA. We discuss the relationship between genes involved in "insect pathogenesis" and those involved in "endophytism" and provide examples of genes with potential importance in lifestyle transitions toward insect pathogenicity. That is, some genes for insect pathogenesis may have been coopted from genes involved in endophytic colonization. Other genes may be multifunctional and serve in both lifestyle capacities. The interactions of EIPF with their host plants are discussed in some detail. The genetic basis for rhizospheric competence, plant communication, and nutrient exchange is examined and we highlight, with examples, the benefits of EIPF to plants, and the potential reservoir of secondary metabolites hidden within these beneficial symbioses. PMID:27131324

  5. Linking energetics and overwintering in temperate insects.

    PubMed

    Sinclair, Brent J

    2015-12-01

    Overwintering insects cannot feed, and energy they take into winter must therefore fuel energy demands during autumn, overwintering, warm periods prior to resumption of development in spring, and subsequent activity. Insects primarily consume lipids during winter, but may also use carbohydrate and proteins as fuel. Because they are ectotherms, the metabolic rate of insects is temperature-dependent, and the curvilinear nature of the metabolic rate-temperature relationship means that warm temperatures are disproportionately important to overwinter energy use. This energy use may be reduced physiologically, by reducing the slope or elevation of the metabolic rate-temperature relationship, or because of threshold changes, such as metabolic suppression upon freezing. Insects may also choose microhabitats or life history stages that reduce the impact of overwinter energy drain. There is considerable capacity for overwinter energy drain to affect insect survival and performance both directly (via starvation) or indirectly (for example, through a trade-off with cryoprotection), but this has not been well-explored. Likewise, the impact of overwinter energy drain on growing-season performance is not well understood. I conclude that overwinter energetics provides a useful lens through which to link physiology and ecology and winter and summer in studies of insect responses to their environment. PMID:26615721

  6. Spectroscopic and Functional Characterization of Nitrophorin 7 from the Blood-Feeding Insect Rhodnius prolixus Reveals an Important Role of Its Isoform-Specific N-Terminus for Proper Protein Function†

    PubMed Central

    Knipp, Markus; Yang, Fei; Berry, Robert E.; Zhang, Hongjun; Shokhirev, Maxim N.; Walker, F. Ann

    2008-01-01

    Nitrophorins (NPs) are a class of NO transporting and histamine sequestering heme b proteins that occur in the saliva of the bloodsucking insect Rhodnius prolixus. A detailed study of the newly described member, NP7, is presented herein. NP7 NO association constants KeqIII(NO) reveal a drastic change when the pH is varied from 5.5 (reflecting the insect's saliva) to slightly above plasma pH (7.5) (>109 M−1 → 4.0 × 106 M−1); thus, the protein promotes the storage of NO in the insect's saliva and its release inside the victim's tissue. In contrast to the other nitrophorins NP1-4, histamine sequestering cannot be accomplished in vivo due to the low binding constant KeqIII(histamine) = 105 M−1 compared to [histamine] = 1 − 10 × 10−9 M in the blood. A major part of this study deals with the N-terminus 1Leu–Pro–Gly–Glu–Cys5 of NP7, which is not found in NP1-4. Since NP7 has not been isolated from the insects so far, but was recognized in a cDNA library instead, the N-terminal site of signal peptidase cleavage upon protein secretion was predicted by the program SignalP [Andersen, J.F., Gudderra, N.P., Francischetti, I.M.B., Valenzuela, J.G., Ribeiro, J.M.C. (2004) Biochemistry 43, 6987-6994]. In marked contrast to wild-type NP7, NP7(Δ1-3) shows a very high NO-affinity at pH 7.5 (KeqIII(NO)≈ 109 M−1), suggesting that the release of NO in the plasma cannot efficiently be accomplished by the truncated form. Comparison of the reduction potentials of both constructs by spectroelectrochemistry revealed an average increase of +85 mV for various distal ligands bound to the heme-iron when 1Leu–Pro–Gly3 was removed. However, 1H NMR and EPR spectroscopy show that the electronic properties of the FeIII cofactor are similar in both wild-type NP7 and NP7(Δ1-3). Further, thermal denaturation that revealed a higher stability of wild-type NP7 compared to NP7(Δ1-3), in combination with a homology model based on the NP2 crystal structure (RMSD = 0.39

  7. Enterococci in Insects

    PubMed Central

    Martin, Jonathan D.; Mundt, J. Orvin

    1972-01-01

    Enterococci were obtained from 213 of 403 insects cultured during a 14-month period, in numbers from 103 to 3 × 107/g of insect. Insects were taken only from nonurban, wild, and cultivated fields and woods. In species of insects carrying them, enterococci were not always present in every individual cultured, and often more than one species of enterococcus occurred within a species. Enterococci were obtained from certain insects taken in the field during the dormant season, suggesting their role as overwintering agents. They were generally present in species feeding on nectar, succulent plant parts, and on and ir forest litter, but not from insects feeding on less succulent leaves and stems. Streptococcus faecalis was recovered from 32%, Streptococcus faecium from 22.4%, and Streptococcus faecium var. casseliflavus from 43.5% of members of the 37 taxa of insects. S. faecalis and S. faecium var. casseliflavus exhibit a high percent of conformity to the properties published for them. The heterogeneity in properties of S. faecium is similar to that found for the species taken from plants. Many fail to grow in broth at 45 C or in broth containing 6.5% NaCl; 50% of the cultures ferment both melezitose and melibiose, and a few ferment neither sugar. The remainder ferment melibiose only. Failure to reduce methylene blue in milk by S. faecalis and S. faecium is correlated with the inability to ferment lactose. More than 93% of the cultures of S. faecalis digest casein in milk from the top downward, following the production of a soft, flowing curd. Because this property is not characteristic of S. faecalis taken from humans, the reaction in litmus milk is suggested as a means of differentiation between cultures of remote and innocent origin in nature and recent, human pollution. PMID:4628796

  8. Insect pest management in forest ecosystems

    NASA Astrophysics Data System (ADS)

    Dahlsten, Donald L.; Rowney, David L.

    1983-01-01

    Understanding the role of insects in forest ecosystems is vital to the development of environmentally and economically sound pest management strategies in forestry Most of the research on forest insects has been confined to phytophagous species associated with economically important tree species The roles of most other insects in forest environments have generally been ignored, including the natural enemies and associates of phytophagous species identified as being important In the past few years several investigations have begun to reevaluate the role of phytophagous species responsible for perturbation in forest ecosystems, and it appears that these species may be playing an important role in the primary productivity of those ecosystems Also, there is an increasing awareness that forest pest managers have been treating the symptoms and not the causes of the problems in the forest Many insect problems are associated with poor sites or sites where trees are growing poorly because of crowding As a result, there is considerable emphasis on the hazard rating of stands of trees for their susceptibility to various phytophagous insects The next step is to manipulate forest stands to make them less susceptible to forest pest complexes A thinning study in California is used as an example and shows that tree mortality in ponderosa pine ( Pinus ponderosa) attributable to the western pine beetle ( Dendroctonus brevicomis) can be reduced by commercial thinning to reduce stocking

  9. Overview of Mosquito Research Programs at the United States Department of Agriculture - Agricultural Research Service, Center for Medical, Agricultural & Veterinary Entomology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Center for Medical, Agricultural, and Veterinary Entomology (CMAVE), a U.S. Department of AgricultureAgricultural Research Service laboratory, was established in World War II to produce products to protect military personnel against insect vector of disease. Currently the mission of CMAVE is ...

  10. Flight stability analysis under changes in insect morphology

    NASA Astrophysics Data System (ADS)

    Noest, Robert; Wang, Z. Jane

    2015-11-01

    Insect have an amazing ability to control their flight, being able to perform both fast aerial maneuvers and stable hovering. The insect's neural system has developed various mechanism by which it can control these flying feats, but we expect that insect morphology is equally important in facilitating the aerial control. We perform a computational study using a quasi-steady instantaneous flapping flight model which allows us to freely adapt the insect's morphological parameters. We picked a fruit fly as the basis for the body shape and wing motion, and study the effect of changes to the morphology for a range of wing stroke amplitudes. In each case we determine the periodic flight mode, with the period equal to a single wing beat, and do a Floquet stability analysis of the flight. To interpret our results we will compare the changed morphology to related insects. We discuss the implications of the insects location on the stability diagram.

  11. The diversity of insect antiviral immunity: insights from viruses.

    PubMed

    Marques, João T; Imler, Jean-Luc

    2016-08-01

    Insects represent over 70% of all animal species. Recent virome analyses reveal unprecedented genetic diversity of insect viruses, which appears to match that of their hosts. Thus, insect-virus interactions may provide information on a vast repertoire of antiviral immune mechanisms. Tapping into this diversity is challenging because of several constraints imposed by the uniqueness of each insect model. Nevertheless, it is clear that many conserved and divergent pathways participate in the control of viral infection in insects. Co-evolution between hosts and viruses favors the development of immune evasion mechanisms by the pathogen. Viral suppressors can offer unique perspective on host pathways and emphasize the importance of RNA interference, apoptosis, but also NF-κB pathways and translation control in insect antiviral immunity. PMID:27232381

  12. Plant rhabdoviruses: new insights and research needs in the interplay of negative-strand RNA viruses with plant and insect hosts.

    PubMed

    Mann, Krin S; Dietzgen, Ralf G

    2014-08-01

    Rhabdoviruses are taxonomically classified in the family Rhabdoviridae, order Mononegavirales. As a group, rhabdoviruses can infect plants, invertebrates and vertebrates. Plant cyto- and nucleorhabdoviruses infect a wide variety of species across both monocot and dicot families, including agriculturally important crops such as lettuce, wheat, barley, rice, maize, potato and tomato. Plant rhabdoviruses are transmitted by and replicate in hemipteran insects such as aphids (Aphididae), leafhoppers (Cicadellidae), or planthoppers (Delphacidae). These specific interactions between plants, viruses and insects offer new insights into host adaptation and molecular virus evolution. This review explores recent advances as well as knowledge gaps in understanding of replication, RNA silencing suppression and movement of plant rhabdoviruses with respect to both plant and insect hosts. PMID:24610553

  13. Evolution of the teachings of chemistry in the new degrees of School of Agricultural Engineering and its importance in the acquisition of competencies

    NASA Astrophysics Data System (ADS)

    Arce, Augusto; Tarquis, Ana M.; Castellanos, Maria Teresa; Requejo, Maria Isabel; Cartagena, Maria Carmen

    2014-05-01

    The academic year 2012-13 is the third year of implementation of the Bologna process in ETSI Agricultural for the subjects Chemistry I and Chemistry II in the new four Degrees: Graduate in Engineering and Agricultural Science, Food Engineering Graduate, Graduate in Engineering Environmental and Biotechnology graduate. We have implemented new interactive methodologies in the teaching-learning process based on the use of the virtual platform of the UPM, and teaching support materials and new laboratory practice developing has. It has also launched new continuous assessment systems that promote active student participation. A comparative study of academic achievements by students of the new grades in the subjects of chemistry during the last three academic years was performed to correlating the results obtained, the success rate and the drop out, and compare with the level of prior knowledge to those entering students. Possible solutions to try and fix these results in future courses are proposed Finally, the general competencies that contribute this course, how they are acquired and how they should be evaluated correctly are indicated. Acknowledgments: Innovation educative projects Nº IE02054-11/12 UPM. 2012

  14. Behavioral Immunity in Insects

    PubMed Central

    de Roode, Jacobus C.; Lefèvre, Thierry

    2012-01-01

    Parasites can dramatically reduce the fitness of their hosts, and natural selection should favor defense mechanisms that can protect hosts against disease. Much work has focused on understanding genetic and physiological immunity against parasites, but hosts can also use behaviors to avoid infection, reduce parasite growth or alleviate disease symptoms. It is increasingly recognized that such behaviors are common in insects, providing strong protection against parasites and parasitoids. We review the current evidence for behavioral immunity in insects, present a framework for investigating such behavior, and emphasize that behavioral immunity may act through indirect rather than direct fitness benefits. We also discuss the implications for host-parasite co-evolution, local adaptation, and the evolution of non-behavioral physiological immune systems. Finally, we argue that the study of behavioral immunity in insects has much to offer for investigations in vertebrates, in which this topic has traditionally been studied. PMID:26466629

  15. Insect Repellents: Protect Your Child from Insect Bites

    MedlinePlus

    ... Español Text Size Email Print Share Choosing an Insect Repellent for Your Child Page Content Article Body Mosquitoes , ... sunscreen needs to be reapplied often. Reactions to Insect Repellents If you suspect that your child is having ...

  16. Cognition in insects

    PubMed Central

    Webb, Barbara

    2012-01-01

    A traditional view of cognition is that it involves an internal process that represents, tracks or predicts an external process. This is not a general characteristic of all complex neural processing or feedback control, but rather implies specific forms of processing giving rise to specific behavioural capabilities. In this paper, I will review the evidence for such capabilities in insect navigation and learning. Do insects know where they are, or do they only know what to do? Do they learn what stimuli mean, or do they only learn how to behave? PMID:22927570

  17. Milkweed: A resource for increasing stink bug parasitism and aiding insect pollinator and monarch butterfly conservation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The flowers of milkweed species can produce a rich supply of nectar, and therefore, planting an insecticide-free milkweed habitat in agricultural farmscapes could possibly conserve monarch butterflies, bees and other insect pollinators, as well as enhance parasitism of insect pests. In peanut-cotton...

  18. Structure and function of an insect α-carboxylesterase (αEsterase7) associated with insecticide resistance

    PubMed Central

    Jackson, Colin J.; Liu, Jian-Wei; Carr, Paul D.; Younus, Faisal; Coppin, Chris; Meirelles, Tamara; Lethier, Mathilde; Pandey, Gunjan; Ollis, David L.; Russell, Robyn J.; Weik, Martin; Oakeshott, John G.

    2013-01-01

    Insect carboxylesterases from the αEsterase gene cluster, such as αE7 (also known as E3) from the Australian sheep blowfly Lucilia cuprina (LcαE7), play an important physiological role in lipid metabolism and are implicated in the detoxification of organophosphate (OP) insecticides. Despite the importance of OPs to agriculture and the spread of insect-borne diseases, the molecular basis for the ability of α-carboxylesterases to confer OP resistance to insects is poorly understood. In this work, we used laboratory evolution to increase the thermal stability of LcαE7, allowing its overexpression in Escherichia coli and structure determination. The crystal structure reveals a canonical α/β-hydrolase fold that is very similar to the primary target of OPs (acetylcholinesterase) and a unique N-terminal α-helix that serves as a membrane anchor. Soaking of LcαE7 crystals in OPs led to the capture of a crystallographic snapshot of LcαE7 in its phosphorylated state, which allowed comparison with acetylcholinesterase and rationalization of its ability to protect insects against the effects of OPs. Finally, inspection of the active site of LcαE7 reveals an asymmetric and hydrophobic substrate binding cavity that is well-suited to fatty acid methyl esters, which are hydrolyzed by the enzyme with specificity constants (∼106 M−1 s−1) indicative of a natural substrate. PMID:23733941

  19. A computer model of insect traps in a landscape

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Attractant-based trap networks are important elements of invasive insect detection, pest control, and basic research programs. We present a landscape-level spatially explicit model of trap networks that incorporates variable attractiveness of traps and a movement model for insect dispersion. We desc...

  20. The use of phytophagous insects in biological control (in French)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Phytophagous insects play an important role in classical biological control. This chapter is divided into 6 paragraphs: 1) we present firstly the history and the context of biocontrol, 2) we detail the impact of herbivory on the target plants; 3) we specify the different groups of insects that have ...

  1. Differences in autonomy of humans and ultrasocial insects.

    PubMed

    Vranka, Marek; Bahník, Štěpán

    2016-01-01

    The target article is built on an analogy between humans and ultrasocial insects. We argue that there are many important limitations to the analogy that make any possible inferences from the analogy questionable. We demonstrate the issue using an example of the difference between a loss of autonomy in humans and in social insects. PMID:27562636

  2. Destructive and Useful Insects in Sweetpotato Breeding Nurseries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sweetpotato polycross nurseries depend on natural pollination by bees and other insects. Unpollinated flowers dehise and do not produce seeds, thus it is important to protect natural bee populations in ensure maximum seed production. There are several other insect species that frequent sweetpotato...

  3. Minute bug with enormous impacts on insect pests

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Minute pirate bugs (Orius spp.) are common and abundant insect predators that can be found in cotton and many other field crops in Arizona and the western U.S. They are important predators of a variety of insect and mite pests in western crops and can help to suppress pest populations and thus cont...

  4. Rapid evolution of immune proteins in social insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In social insects the existence of behavioral traits connected to defense against pathogens manifests the importance of pathogens in the evolution of social insects. However, very little is known how the pathogen pressure has affected the evolution of genes involved in the innate immune system in so...

  5. Tn5 as an insect gene vector.

    PubMed

    Rowan, Kathryn H; Orsetti, Jamison; Atkinson, Peter W; O'Brochta, David A

    2004-07-01

    The purpose of this study was to explore alternatives to insect-derived transposable elements as insect gene vectors with the intention of improving existing insect transgenesis methods. The mobility properties of the bacterial transposon, Tn5, were tested in mosquitoes using a transient transposable element mobility assay and by attempting to create transgenic insects. Tn5 synaptic complexes were assembled in vitro in the absence of Mg(2+) and co-injected with a target plasmid into developing yellow fever mosquito, Aedes aegypti, embryos. Target plasmids recovered from embryos a day later were screened for the presence of Tn5. Recombinants (transposition events) were found at a frequency of 1.2 x 10(-3). Some transposition events did not appear to be associated with canonical 9 bp direct duplications at the site of insertion and also were associated with either deletions or rearrangements. A Tn5 element containing the brain-specific transgene, 3 x P3DsRed, was assembled into synaptic complexes in vitro and injected into pre-blastoderm embryos of Ae. aegypti. Of the approximately 900 embryos surviving injection and developing into adults, two produced transgenic progeny. Both transgenic events involved the co-integrations of approximately five elements resulting in nested and tandem arrayed Tn5::3 x P3DsRed elements. This study extends the known host range of Tn5 to insects and makes available to insect biologists and others another eukaryotic genome-manipulation tool. The hyperactivity of synaptic complexes may be responsible for the unusual clustering of elements and managing this aspect of the element's behavior will be important in future applications of this technology to insects. PMID:15242711

  6. Protecting Yourself from Stinging Insects

    MedlinePlus

    ... at risk of being stung by flying insects (bees, wasps, and hornets) and fire ants. While most ... by several stinging insects, run to get away. (Bees release a chemical when they sting, which attracts ...

  7. Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance.

    PubMed

    Badran, Ahmed H; Guzov, Victor M; Huai, Qing; Kemp, Melissa M; Vishwanath, Prashanth; Kain, Wendy; Nance, Autumn M; Evdokimov, Artem; Moshiri, Farhad; Turner, Keith H; Wang, Ping; Malvar, Thomas; Liu, David R

    2016-05-01

    The Bacillus thuringiensis δ-endotoxins (Bt toxins) are widely used insecticidal proteins in engineered crops that provide agricultural, economic, and environmental benefits. The development of insect resistance to Bt toxins endangers their long-term effectiveness. Here we have developed a phage-assisted continuous evolution selection that rapidly evolves high-affinity protein-protein interactions, and applied this system to evolve variants of the Bt toxin Cry1Ac that bind a cadherin-like receptor from the insect pest Trichoplusia ni (TnCAD) that is not natively bound by wild-type Cry1Ac. The resulting evolved Cry1Ac variants bind TnCAD with high affinity (dissociation constant Kd = 11-41 nM), kill TnCAD-expressing insect cells that are not susceptible to wild-type Cry1Ac, and kill Cry1Ac-resistant T. ni insects up to 335-fold more potently than wild-type Cry1Ac. Our findings establish that the evolution of Bt toxins with novel insect cell receptor affinity can overcome insect Bt toxin resistance and confer lethality approaching that of the wild-type Bt toxin against non-resistant insects. PMID:27120167

  8. Investigation--Insects!

    ERIC Educational Resources Information Center

    Fay, Janice

    2000-01-01

    Presents activities on insects for second grade students. In the first activity, students build a butterfly garden. In the second activity, students observe stimuli reactions with mealworms in the larval stage. Describes the assessment process and discusses the effects of pollution on living things. (YDS)

  9. Fluorescence in insects

    NASA Astrophysics Data System (ADS)

    Welch, Victoria L.; Van Hooijdonk, Eloise; Intrater, Nurit; Vigneron, Jean-Pol

    2012-10-01

    Fluorescent molecules are much in demand for biosensors, solar cells, LEDs and VCSEL diodes, therefore, considerable efforts have been expended in designing and tailoring fluorescence to specific technical applications. However, naturally occurring fluorescence of diverse types has been reported from a wide array of living organisms: most famously, the jellyfish Aequorea victoria, but also in over 100 species of coral and in the cuticle of scorpions, where it is the rule, rather than the exception. Despite the plethora of known insect species, comparatively few quantitative studies have been made of insect fluorescence. Because of the potential applications of natural fluorescence, studies in this field have relevance to both physics and biology. Therefore, in this paper, we review the literature on insect fluorescence, before documenting its occurrence in the longhorn beetles Sternotomis virescens, Sternotomis variabilis var. semi rufescens, Anoplophora elegans and Stellognatha maculata, the tiger beetles Cicindela maritima and Cicindela germanica and the weevil Pachyrrhynchus gemmatus purpureus. Optical features of insect fluorescence, including emitted wavelength, molecular ageing and naturally occurring combinations of fluorescence with bioluminescence and colour-producing structures are discussed.

  10. Insects. Thematic Unit.

    ERIC Educational Resources Information Center

    Gosnell, Kathee

    This book is a captivating whole-language thematic unit about the study of insects, relating it to our understanding of the past and our hopes for using our knowledge in the present to balance the ecosystem in the future. It contains a wide variety of lesson ideas and reproducible pages designed for use with intermediate students. At its core,…

  11. SOCIAL INSECT PHEROMONES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Social insects include the social Hymenoptera (Formicidae, ants; Apidae, bees; Vespidae, wasps) and Isoptera (Termitidae, termites). Social interactions are required for effective food retrieval, brood and queen care, regulation of caste (sexuals/workers), recognition and exclusion of non-nestmates,...

  12. People and Insects.

    ERIC Educational Resources Information Center

    NatureScope, 1985

    1985-01-01

    Provides: (1) background information on how insects affect human lives, both positively and negatively, and on integrated pest management strategies; (2) student activities; and (3) materials (ready-to-copy games, puzzles, coloring pages, worksheets, and/or mazes). Each activity includes an objective, recommended age level(s), subject area(s),…

  13. Insect mass production technologies

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insects provide a very promising alternative for the future production of animal protein. Their nutritional value in conjunction with their food conversion efficiency and low water requirements, make them a more sustainable choice for the production of food and animal origin. However, to realize the...

  14. Recycled Insect Models

    ERIC Educational Resources Information Center

    Rule, Audrey C.; Meyer, Mary Ann

    2007-01-01

    This article presents an engaging activity in which high school students use a dichotomous key to guide the creation and classification of model insects from recycled plastic lids and containers. Besides teaching the use of a dichotomous key and the effect of evolutionary descent upon groupings of organisms, this activity focuses on an…

  15. Dispersal of forest insects

    NASA Technical Reports Server (NTRS)

    Mcmanus, M. L.

    1979-01-01

    Dispersal flights of selected species of forest insects which are associated with periodic outbreaks of pests that occur over large contiguous forested areas are discussed. Gypsy moths, spruce budworms, and forest tent caterpillars were studied for their massive migrations in forested areas. Results indicate that large dispersals into forested areas are due to the females, except in the case of the gypsy moth.

  16. Corn Insect Pests

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Historically, the major corn insect pests have been corn rootworms (northern and western), European corn borer, and black cutworm. Bt-corn hybrids are effective against most of these pests. However, Bt-corn hybrids are not effective against corn leaf aphid, corn root aphid, sap beetles, corn rootwor...

  17. Colour constancy in insects.

    PubMed

    Chittka, Lars; Faruq, Samia; Skorupski, Peter; Werner, Annette

    2014-06-01

    Colour constancy is the perceptual phenomenon that the colour of an object appears largely unchanged, even if the spectral composition of the illuminating light changes. Colour constancy has been found in all insect species so far tested. Especially the pollinating insects offer a remarkable opportunity to study the ecological significance of colour constancy since they spend much of their adult lives identifying and choosing between colour targets (flowers) under continuously changing ambient lighting conditions. In bees, whose colour vision is best studied among the insects, the compensation provided by colour constancy is only partial and its efficiency depends on the area of colour space. There is no evidence for complete 'discounting' of the illuminant in bees, and the spectral composition of the light can itself be used as adaptive information. In patchy illumination, bees adjust their spatial foraging to minimise transitions between variously illuminated zones. Modelling allows the quantification of the adaptive benefits of various colour constancy mechanisms in the economy of nature. We also discuss the neural mechanisms and cognitive operations that might underpin colour constancy in insects. PMID:24647930

  18. Radar cross section of insects

    NASA Astrophysics Data System (ADS)

    Riley, J. R.

    1985-02-01

    X-band measurements of radar cross section as a function of the angle between insect body axis and the plane of polarization are presented. A finding of particular interest is that in larger insects, maximum cross section occurs when the E-vector is perpendicular to the body axis. A new range of measurements on small insects (aphids, and planthoppers) is also described, and a comprehensive summary of insect cross-section data at X-band is given.

  19. Laser system for identification, tracking, and control of flying insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Flying insects are common vectors for transmission of pathogens and inflict significant harm on humans in large parts of the developing world. Besides the direct impact to humans, these pathogens also cause harm to crops and result in agricultural losses. Here, we present a laser-based system that c...

  20. OMIGA: Optimized Maker-Based Insect Genome Annotation.

    PubMed

    Liu, Jinding; Xiao, Huamei; Huang, Shuiqing; Li, Fei

    2014-08-01

    Insects are one of the largest classes of animals on Earth and constitute more than half of all living species. The i5k initiative has begun sequencing of more than 5,000 insect genomes, which should greatly help in exploring insect resource and pest control. Insect genome annotation remains challenging because many insects have high levels of heterozygosity. To improve the quality of insect genome annotation, we developed a pipeline, named Optimized Maker-Based Insect Genome Annotation (OMIGA), to predict protein-coding genes from insect genomes. We first mapped RNA-Seq reads to genomic scaffolds to determine transcribed regions using Bowtie, and the putative transcripts were assembled using Cufflink. We then selected highly reliable transcripts with intact coding sequences to train de novo gene prediction software, including Augustus. The re-trained software was used to predict genes from insect genomes. Exonerate was used to refine gene structure and to determine near exact exon/intron boundary in the genome. Finally, we used the software Maker to integrate data from RNA-Seq, de novo gene prediction, and protein alignment to produce an official gene set. The OMIGA pipeline was used to annotate the draft genome of an important insect pest, Chilo suppressalis, yielding 12,548 genes. Different strategies were compared, which demonstrated that OMIGA had the best performance. In summary, we present a comprehensive pipeline for identifying genes in insect genomes that can be widely used to improve the annotation quality in insects. OMIGA is provided at http://ento.njau.edu.cn/omiga.html . PMID:24609470

  1. Insect vision as model for machine vision

    NASA Astrophysics Data System (ADS)

    Osorio, D.; Sobey, Peter J.

    1992-11-01

    The neural architecture, neurophysiology and behavioral abilities of insect vision are described, and compared with that of mammals. Insects have a hardwired neural architecture of highly differentiated neurons, quite different from the cerebral cortex, yet their behavioral abilities are in important respects similar to those of mammals. These observations challenge the view that the key to the power of biological neural computation is distributed processing by a plastic, highly interconnected, network of individually undifferentiated and unreliable neurons that has been a dominant picture of biological computation since Pitts and McCulloch's seminal work in the 1940's.

  2. Acoustic Detection of Insects in Palm Trees

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Commercial-crop and ornamental palm trees serve important functions in tropical and subtropical regions of the world, and considerable precautions are taken each year to identify and control infestations of a variety of different insect pests. Large weevils, including the red palm weevil and the co...

  3. Almond Production Manual Chapter: Insects and Mites

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The navel orangeworm, Amyelois transitella (Walker), is the most important insect pest of almond in California and can cost as much as $500 dollars per acre to control when the costs of insecticides and sanitation are included. It is a native of the southwestern United States and Mexico and was firs...

  4. Insects: Little Things That Run the World

    ERIC Educational Resources Information Center

    Tilley, Luke

    2014-01-01

    Insects are easily the most abundant and diverse group of animals, with over 24,000 species in the UK alone. They can be found in almost every habitat on Earth and are fundamentally important to ecology, conservation, food production, animal and human health, and biodiversity. They are a prominent feature of almost every food web in the UK and…

  5. Insect Control (II): Hormones and Viruses

    ERIC Educational Resources Information Center

    Marx, Jean L.

    1973-01-01

    Discusses research in the use of hormones and viruses to control insect populations. Although entomologists do not think that pheromones, hormones, and viruses will completely replace more conventional chemical insecticides, they will become increasingly important and will reduce our dependence on traditional insecticides. (JR)

  6. Development of Baits for Insect Control

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This article outlines the importance of baits. Baits are formulations that can be used to deliver a toxic chemical or a pathogen (active agent) via ingestion to an insect pest with the goal of killing it. A bait formulations consist of a bait matrix which is the carrier for an active agent. The bait...

  7. The Composite Insect Trap: An Innovative Combination Trap for Biologically Diverse Sampling

    PubMed Central

    Russo, Laura; Stehouwer, Rachel; Heberling, Jacob Mason; Shea, Katriona

    2011-01-01

    Documentation of insect diversity is an important component of the study of biodiversity, community dynamics, and global change. Accurate identification of insects usually requires catching individuals for close inspection. However, because insects are so diverse, most trapping methods are specifically tailored to a particular taxonomic group. For scientists interested in the broadest possible spectrum of insect taxa, whether for long term monitoring of an ecosystem or for a species inventory, the use of several different trapping methods is usually necessary. We describe a novel composite method for capturing a diverse spectrum of insect taxa. The Composite Insect Trap incorporates elements from four different existing trapping methods: the cone trap, malaise trap, pan trap, and flight intercept trap. It is affordable, resistant, easy to assemble and disassemble, and collects a wide variety of insect taxa. Here we describe the design, construction, and effectiveness of the Composite Insect Trap tested during a study of insect diversity. The trap catches a broad array of insects and can eliminate the need to use multiple trap types in biodiversity studies. We propose that the Composite Insect Trap is a useful addition to the trapping methods currently available to ecologists and will be extremely effective for monitoring community level dynamics, biodiversity assessment, and conservation and restoration work. In addition, the Composite Insect Trap will be of use to other insect specialists, such as taxonomists, that are interested in describing the insect taxa in a given area. PMID:21698160

  8. Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusaria

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fusarium (Hypocreales, Nectriaceae) is one of the most economically important and systematically challenging groups of mycotoxigenic phytopathogens and emergent human pathogens. We conducted maximum likelihood (ML), maximum parsimony (MP) and Bayesian (B) analyses on partial RNA polymerase largest (...

  9. Historical and current roles of insects and pathogens in eastern Oregon and Washington forested landscapes. Forest Service general technical report

    SciTech Connect

    Hessburg, P.F.; Mitchell, R.G.; Filip, G.M.

    1994-04-01

    The paper examines, by climax conifer series, historical and current roles of many important pathogens and insects of interior Northwest coniferious forests and their unique responses to changing successional conditions resulting from management. Future research on forest pathogens and insects should address three primary subject areas: insect and pathogen population dynamics in managed and unmanaged forests; ecological roles and effects of native and introduced pathogens and insects; and effects of natural disturbances and management practices on native insects, pathogens, and their natural enemies.

  10. Gut microbes may facilitate insect herbivory of chemically defended plants.

    PubMed

    Hammer, Tobin J; Bowers, M Deane

    2015-09-01

    The majority of insect species consume plants, many of which produce chemical toxins that defend their tissues from attack. How then are herbivorous insects able to develop on a potentially poisonous diet? While numerous studies have focused on the biochemical counter-adaptations to plant toxins rooted in the insect genome, a separate body of research has recently emphasized the role of microbial symbionts, particularly those inhabiting the gut, in plant-insect interactions. Here we outline the "gut microbial facilitation hypothesis," which proposes that variation among herbivores in their ability to consume chemically defended plants can be due, in part, to variation in their associated microbial communities. More specifically, different microbes may be differentially able to detoxify compounds toxic to the insect, or be differentially resistant to the potential antimicrobial effects of some compounds. Studies directly addressing this hypothesis are relatively few, but microbe-plant allelochemical interactions have been frequently documented from non-insect systems-such as soil and the human gut-and thus illustrate their potential importance for insect herbivory. We discuss the implications of this hypothesis for insect diversification and coevolution with plants; for example, evolutionary transitions to host plant groups with novel allelochemicals could be initiated by heritable changes to the insect microbiome. Furthermore, the ecological implications extend beyond the plant and insect herbivore to higher trophic levels. Although the hidden nature of microbes and plant allelochemicals make their interactions difficult to detect, recent molecular and experimental techniques should enable research on this neglected, but likely important, aspect of insect-plant biology. PMID:25936531

  11. Land-use history alters contemporary insect herbivore community composition and decouples plant-herbivore relationships.

    PubMed

    Hahn, Philip G; Orrock, John L

    2015-05-01

    Past land use can create altered soil conditions and plant communities that persist for decades, although the effects of these altered conditions on consumers are rarely investigated. Using a large-scale field study at 36 sites in longleaf pine (Pinus palustris) woodlands, we examined whether historic agricultural land use leads to differences in the abundance and community composition of insect herbivores (grasshoppers, families Acrididae and Tettigoniidae). We measured the cover of six plant functional groups and several environmental variables to determine whether historic agricultural land use affects the relationships between plant cover or environmental conditions and grasshopper assemblages. Land-use history had taxa-specific effects and interacted with herbaceous plant cover to alter grasshopper abundances, leading to significant changes in community composition. Abundance of most grasshopper taxa increased with herbaceous cover in woodlands with no history of agriculture, but there was no relationship in post-agricultural woodlands. We also found that grasshopper abundance was negatively correlated with leaf litter cover. Soil hardness was greater in post-agricultural sites (i.e. more compacted) and was associated with grasshopper community composition. Both herbaceous cover and leaf litter cover are influenced by fire frequency, suggesting a potential indirect role of fire on grasshopper assemblages. Our results demonstrate that historic land use may create persistent differences in the composition of grasshopper assemblages, while contemporary disturbances (e.g. prescribed fire) may be important for determining the abundance of grasshoppers, largely through the effect of fire on plants and leaf litter. Therefore, our results suggest that changes in the contemporary management regimes (e.g. increasing prescribed fire) may not be sufficient to shift the structure of grasshopper communities in post-agricultural sites towards communities in non-agricultural

  12. Spatial-Temporal Models of Insect Growth, Diffusion and Derivative Pricing

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insect derivatives represent an important innovation in specialty crop risk management. An active over-the-counter market in insect derivatives will require a transparent pricing method. The paper develops an econometric model of the spatio-temporal process underlying a particular insect populatio...

  13. Make your trappings count: The mathematics of pest insect monitoring. Comment on “Multiscale approach to pest insect monitoring: Random walks, pattern formation, synchronization, and networks” by Petrovskii et al.

    NASA Astrophysics Data System (ADS)

    Blasius, Bernd

    2014-09-01

    Since the beginnings of agriculture the production of crops is characterized by an ongoing battle between farmers and pests [1]. Already during biblical times swarms of the desert locust, Schistocerca gregaria, were known as major pest that can devour a field of corn within an hour. Even today, harmful organisms have the potential to threaten food production worldwide. It is estimated that about 37% of all potential crops are destroyed by pests. Harmful insects alone destroy 13%, causing financial losses in the agricultural industry of millions of dollars each year [2-4]. These numbers emphasize the importance of pest insect monitoring as a crucial step of integrated pest management [1]. The main approach to gain information about infestation levels is based on trapping, which leads to the question of how to extrapolate the sparse population counts at singularly disposed traps to a spatial representation of the pest species distribution. In their review Petrovskii et al. provide a mathematical framework to tackle this problem [5]. Their analysis reveals that this seemingly inconspicuous problem gives rise to surprisingly deep mathematical challenges that touch several modern contemporary concepts of statistical physics and complex systems theory. The review does not aim for a collection of numerical recipes to support crop growers in the analysis of their trapping data. Instead the review identifies the relevant biological and physical processes that are involved in pest insect monitoring and it presents the mathematical techniques that are required to capture these processes.

  14. DIELECTRIC PROPERTIES OF MATERIALS AND RELATED AGRICULTURAL APPLICATIONS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Potential Agricultural applications for RF and microwave energy include selective dielectric heating of insects in grain, treatment of seed to improve germination, and conditioning of products to improve nutritional value and shelf life. Measurement applications include permittivity-density relatio...

  15. Oviposition Deterrents in Herbivorous Insects and their potential use in Integrated Pest Management.

    PubMed

    Kumari, Archana; Kaushik, Nutan

    2016-03-01

    In the life cycle of insects, oviposition is an important phenomenon, and it is influenced by many intrinsic and extrinsic factors, especially in relation to suitable hosts for completion of their life-cycle. Oviposition deterrents which deter an insect from laying eggs are important in the management of insect pests. Proper understanding of these deterrents shall provide necessary insight into new vistas for Insect Pest Management. Chemicals from plants and insects play an important role in attracting phytophagous insects for selecting host for oviposition. Considerable research has been done on oviposition deterrents and their mode of actions. In the present review, we have consolidated the updated information on this important aspect of insect behavior. PMID:27145629

  16. Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops

    PubMed Central

    Mondal, Hossain Ali; Chakraborti, Dipankar; Majumder, Pralay; Roy, Pampa; Roy, Amit; Bhattacharya, Swati Gupta; Das, Sampa

    2011-01-01

    Background Mannose-binding Allium sativum leaf agglutinin (ASAL) is highly antinutritional and toxic to various phloem-feeding hemipteran insects. ASAL has been expressed in a number of agriculturally important crops to develop resistance against those insects. Awareness of the safety aspect of ASAL is absolutely essential for developing ASAL transgenic plants. Methodology/Principal Findings Following the guidelines framed by the Food and Agriculture Organization/World Health Organization, the source of the gene, its sequence homology with potent allergens, clinical tests on mammalian systems, and the pepsin resistance and thermostability of the protein were considered to address the issue. No significant homology to the ASAL sequence was detected when compared to known allergenic proteins. The ELISA of blood sera collected from known allergy patients also failed to show significant evidence of cross-reactivity. In vitro and in vivo assays both indicated the digestibility of ASAL in the presence of pepsin in a minimum time period. Conclusions/Significance With these experiments, we concluded that ASAL does not possess any apparent features of an allergen. This is the first report regarding the monitoring of the allergenicity of any mannose-binding monocot lectin having insecticidal efficacy against hemipteran insects. PMID:22110739

  17. Expressing the sense of the House of Representatives that specialty crops are a vital part of agriculture in the United States, that the Committee on Agriculture should propose funding for programs that support specialty crops priorities, and that legislation should be passed that includes funding reflecting specialty crops as a growing and important part of United States agriculture.

    THOMAS, 113th Congress

    Rep. DelBene, Suzan K. [D-WA-1

    2013-04-25

    05/03/2013 Referred to the Subcommittee on Horticulture, Research, Biotechnology, and Foreign Agriculture. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  18. On quantifying insect movements

    SciTech Connect

    Wiens, J.A.; Crist, T.O. ); Milne, B.T. )

    1993-08-01

    We elaborate on methods described by Turchin, Odendaal Rausher for quantifying insect movement pathways. We note the need to scale measurement resolution to the study insects and the questions being asked, and we discuss the use of surveying instrumentation for recording sequential positions of individuals on pathways. We itemize several measures that may be used to characterize movement pathways and illustrate these by comparisons among several Eleodes beetles occurring in shortgrass steppe. The fractal dimension of pathways may provide insights not available from absolute measures of pathway configuration. Finally, we describe a renormalization procedure that may be used to remove sequential interdependence among locations of moving individuals while preserving the basic attributes of the pathway.

  19. The Importance of Considering the Temporal Distribution of Climate Variables for Ecological-Economic Modeling to Calculate the Consequences of Climate Change for Agriculture

    NASA Astrophysics Data System (ADS)

    Plegnière, Sabrina; Casper, Markus; Hecker, Benjamin; Müller-Fürstenberger, Georg

    2014-05-01

    The basis of many models to calculate and assess climate change and its consequences are annual means of temperature and precipitation. This method leads to many uncertainties especially at the regional or local level: the results are not realistic or too coarse. Particularly in agriculture, single events and the distribution of precipitation and temperature during the growing season have enormous influences on plant growth. Therefore, the temporal distribution of climate variables should not be ignored. To reach this goal, a high-resolution ecological-economic model was developed which combines a complex plant growth model (STICS) and an economic model. In this context, input data of the plant growth model are daily climate values for a specific climate station calculated by the statistical climate model (WETTREG). The economic model is deduced from the results of the plant growth model STICS. The chosen plant is corn because corn is often cultivated and used in many different ways. First of all, a sensitivity analysis showed that the plant growth model STICS is suitable to calculate the influences of different cultivation methods and climate on plant growth or yield as well as on soil fertility, e.g. by nitrate leaching, in a realistic way. Additional simulations helped to assess a production function that is the key element of the economic model. Thereby the problems when using mean values of temperature and precipitation in order to compute a production function by linear regression are pointed out. Several examples show why a linear regression to assess a production function based on mean climate values or smoothed natural distribution leads to imperfect results and why it is not possible to deduce a unique climate factor in the production function. One solution for this problem is the additional consideration of stress indices that show the impairment of plants by water or nitrate shortage. Thus, the resulting model takes into account not only the ecological

  20. [Protection against insects].

    PubMed

    Rudin, W

    2005-11-01

    Successful protection against haematophagous insects and ticks, especially in areas where transmission of diseases occurs, requires a consistent application of a combination of appropriate measures. However, this can never substitute a chemoprophylaxis. Which measures have to be used depends on the circumstances under which they have to work. Indoor, physical means such as mosquito-screens on doors and windows, air-conditioners, and bed nets can be used to keep the insects away. These measures can be supplemented or supported by insecticides used as knock-down sprays, by electrical evaporation or for the treatment of screens and bed nets. In the field, if it is not possible to avoid mosquito-areas during phases of activity, appropriate clothing and repellents must provide the protection. Bright, wide pants and shirts of dense weaving covering as much skin as bearable should be preferred. Repellents are sprays, lotions, milks or creams which are evenly applied to the skin to prevent insects from biting. They contain synthetic or natural active substances of substantially varying effectiveness. The gold standard since about 60 years is diethylbenzamine (DEET). There are a few other active substances with a lower risk of side effects, however, combined with a lower effectiveness mainly on people with a high attractiveness for mosquitoes. Products containing an extract of Eucalyptus citriodora provide the best protection amongst those with natural active substances. Wearing bracelets or necklaces treated with repellents, acoustic devices (buzzers), electrocuters, topical or systemic Vitamin B1 or eating garlic are useless measures to prevent insects from biting. PMID:16350532

  1. Fatigue of insect cuticle.

    PubMed

    Dirks, Jan-Henning; Parle, Eoin; Taylor, David

    2013-05-15

    Many parts of the insect exoskeleton experience repeated cyclic loading. Although the cuticle of insects and other arthropods is the second most common natural composite material in the world, so far nothing is known about its fatigue properties, despite the fact that fatigue undoubtedly limits the durability of body parts in vivo. For the first time, we here present experimental fatigue data of insect cuticle. Using force-controlled cyclic loading, we determined the number of cycles to failure for hind legs (tibiae) and hind wings of the locust Schistocerca gregaria, as a function of the applied cyclic stress. Our results show that, although both are made from cuticle, these two body parts behave very differently. Wing samples showed a large fatigue range, failing after 100,000 cycles when we applied 46% of the stress needed for instantaneous failure [the ultimate tensile strength (UTS)]. Legs, in contrast, were able to sustain a stress of 76% of the UTS for the same number of cycles to failure. This can be explained by the difference in the composition and structure of the material, two factors that, amongst others, also affect the well-known behaviour of engineering composites. Final failure of the tibiae occurred via one of two different failure modes--propagation in tension or buckling in compression--indicating that the tibia is 'optimized' by evolution to resist both failure modes equally. These results are further discussed in relation to the evolution and normal use of these two body parts. PMID:23393276

  2. Escape behaviors in insects.

    PubMed

    Card, Gwyneth M

    2012-04-01

    Escape behaviors are, by necessity, fast and robust, making them excellent systems with which to study the neural basis of behavior. This is especially true in insects, which have comparatively tractable nervous systems and members who are amenable to manipulation with genetic tools. Recent technical developments in high-speed video reveal that, despite their short duration, insect escape behaviors are more complex than previously appreciated. For example, before initiating an escape jump, a fly performs sophisticated posture and stimulus-dependent preparatory leg movements that enable it to jump away from a looming threat. This newfound flexibility raises the question of how the nervous system generates a behavior that is both rapid and flexible. Recordings from the cricket nervous system suggest that synchrony between the activity of specific interneuron pairs may provide a rapid cue for the cricket to detect the direction of an approaching predator and thus which direction it should run. Technical advances make possible wireless recording from neurons while locusts escape from a looming threat, enabling, for the first time, a direct correlation between the activity of multiple neurons and the time-course of an insect escape behavior. PMID:22226514

  3. Association of N2-fixing Cyanobacteria and Plants: Towards Novel Symbioses of Agricultural Importance. Final report, 1 April 1996 to 31 May 1997

    SciTech Connect

    Gantar, Miroslav

    1999-03-01

    The goal of this project is to characterize an association that takes place between the roots of wheat and the nitrogen-fixing cyanobacterium Nostoc 2S9. By understanding how the association takes place and the extent to which it permits the growth of the plant without exogenous nitrogenous fertilizer, it may prove possible to increase the benefits of the association and to extend them to other plants of agrinomic importance.

  4. Multilocus phylogeny reveals an association of agriculturally important Fusarium solani species complex (FSSC) 11, and clinically important FSSC 5 and FSSC 3 + 4 with soybean roots in the north central United States.

    PubMed

    Chitrampalam, P; Nelson, B

    2016-02-01

    The Fusarium solani species complex (FSSC) includes important root pathogens of soybean in the United States, but the evolutionary lineages associated with soybean root rot are unknown. A multilocus phylogeny based on 93 isolates from soybean and pea roots from North Dakota and Minnesota revealed that root rot was associated with three known phylogenetic species, FSSC 3 + 4 (=Fusarium falciforme) (3 % of isolates), FSSC 5 (60 %), FSSC 11 (34 %), and one unknown species, FSSC X (2 %). Of these species FSSC 5 and FSSC 3 + 4 are clinically important while FSSC 11 is a plant pathogen. Isolates from FSSC 11 were pathogenic on soybean, dry bean, pea and lentil, and did not grow at 37 °C. However, isolates from FSSC 5 were weakly to non-pathogenic, but grew at 37 °C. Isolates from both FSSC 5 and FSSC 11 were highly resistant to fludioxonil in vitro. This is the first study revealing the pathogenic robustness of FSSC 11 in causing root rot among Fabaceae crops and also the association of clinically important members of the FSSC with roots of a widely grown field crop in the United States. PMID:26671414

  5. Agricultural fields, Khartoum, Sudan, Africa

    NASA Technical Reports Server (NTRS)

    1992-01-01

    This herringbone pattern of irrigated agricultural fields near Khartoum, Sudan (14.5N, 33.5E) is very distinctive in both size and shape. The region contains thousands of these rectangular fields bounded by canals which carry water from both the White and Blue Nile Rivers. A crop rotation system is used so that some fields are in cotton, millit, sorghum or fallow to conserve moisture and control weeds and insects. See also STS049-96-003.

  6. Grassland agriculture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Agriculture in grassland environments is facing multiple stresses from: shifting demographics, declining and fragmented agricultural landscapes, declining environmental quality, variable and changing climate, volatile and increasing energy costs, marginal economic returns, and globalization. Degrad...

  7. Breeding a super nematode for enhanced insect pest suppression

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Entomopathogenic nematodes in the genera Heterorhabditis and Steinernema are important regulators of natural insect populations, and are used commercially as biological control agents for pest suppression. Successful biocontrol applications depend on the introduced organism having an array of benef...

  8. Why Care About Aquatic Insects: Uses, Benefits, and Services

    EPA Science Inventory

    Mayflies and other aquatic insects are common subjects of ecological research, and environmental monitoring and assessment. However, their important role in protecting and restoring aquatic ecosystems is often challenged, because their benefits and services to humans are not obv...

  9. Post-harvest entomology research in the United States Department of Agriculture-Agricultural Research Service.

    PubMed

    Throne, James E; Hallman, Guy J; Johnson, Judy A; Follett, Peter A

    2003-01-01

    This is a review of current post-harvest entomology research conducted by the Agricultural Research Service, the research branch of the US Department of Agriculture. The review covers both durable and perishable commodities. Research on biochemistry, genetics, physiology, monitoring and control of insects infesting stored grain, dried fruits and nuts, and processed commodities is reviewed. Research on development of quarantine treatments, particularly for fruit flies, is also reviewed, including research on thermal and irradiation treatments and a discussion of risk management for quarantine pests. Two areas of research are covered more extensively: a project to map the genome of the red flour beetle, Tribolium castaneum, and the use of near-infrared spectroscopy for detection of hidden infestations in grain, quantification of insect fragments in food, determination of quality in dried fruits, identification of insect species and age-grading insects. Future research directions are identified. PMID:12846312

  10. AGRICULTURAL HEALTH STUDY

    EPA Science Inventory

    The Agricultural Health Study is a large cohort of 90,000 licensed pesticide applicators, plus 30,000 spouses and 20,000 children who are exposed either directly or indirectly. Exposure to pesticides is widespread and is important beyond the agricultural community. Other exposure...

  11. Agriculture in the Midwest

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Agriculture in the Midwest United States (Illinois, Indiana, Iowa, Michigan, Minnesota, Ohio, and Wisconsin) represents one of the most intense areas of agriculture in the world. This area is not only critically important for the United States, but also for world exports of grain and meat for the Un...

  12. Agricultural Production.

    ERIC Educational Resources Information Center

    Lehigh County Area Vocational-Technical School, Schnecksville, PA.

    This brochure describes the philosophy and scope of a secondary-level course in agricultural production. Addressed in the individual units of the course are the following topics: careers in agriculture and agribusiness, animal science and livestock production, agronomy, agricultural mechanics, supervised occupational experience programs, and the…

  13. Fungi with multifunctional lifestyles: endophytic insect pathogenic fungi.

    PubMed

    Barelli, Larissa; Moonjely, Soumya; Behie, Scott W; Bidochka, Michael J

    2016-04-01

    This review examines the symbiotic, evolutionary, proteomic and genetic basis for a group of fungi that occupy a specialized niche as insect pathogens as well as endophytes. We focus primarily on species in the genera Metarhizium and Beauveria, traditionally recognized as insect pathogenic fungi but are also found as plant symbionts. Phylogenetic evidence suggests that these fungi are more closely related to grass endophytes and diverged from that lineage ca. 100 MYA. We explore how the dual life cycles of these fungi as insect pathogens and endophytes are coupled. We discuss the evolution of insect pathogenesis while maintaining an endophytic lifestyle and provide examples of genes that may be involved in the transition toward insect pathogenicity. That is, some genes for insect pathogenesis may have been co-opted from genes involved in endophytic colonization. Other genes may be multifunctional and serve in both lifestyle capacities. We suggest that their evolution as insect pathogens allowed them to effectively barter a specialized nitrogen source (i.e. insects) with host plants for photosynthate. These ubiquitous fungi may play an important role as plant growth promoters and have a potential reservoir of secondary metabolites. PMID:26644135

  14. Determining the Most Important Physiological and Agronomic Traits Contributing to Maize Grain Yield through Machine Learning Algorithms: A New Avenue in Intelligent Agriculture

    PubMed Central

    Shekoofa, Avat; Emam, Yahya; Shekoufa, Navid; Ebrahimi, Mansour; Ebrahimie, Esmaeil

    2014-01-01

    Prediction is an attempt to accurately forecast the outcome of a specific situation while using input information obtained from a set of variables that potentially describe the situation. They can be used to project physiological and agronomic processes; regarding this fact, agronomic traits such as yield can be affected by a large number of variables. In this study, we analyzed a large number of physiological and agronomic traits by screening, clustering, and decision tree models to select the most relevant factors for the prospect of accurately increasing maize grain yield. Decision tree models (with nearly the same performance evaluation) were the most useful tools in understanding the underlying relationships in physiological and agronomic features for selecting the most important and relevant traits (sowing date-location, kernel number per ear, maximum water content, kernel weight, and season duration) corresponding to the maize grain yield. In particular, decision tree generated by C&RT algorithm was the best model for yield prediction based on physiological and agronomical traits which can be extensively employed in future breeding programs. No significant differences in the decision tree models were found when feature selection filtering on data were used, but positive feature selection effect observed in clustering models. Finally, the results showed that the proposed model techniques are useful tools for crop physiologists to search through large datasets seeking patterns for the physiological and agronomic factors, and may assist the selection of the most important traits for the individual site and field. In particular, decision tree models are method of choice with the capability of illustrating different pathways of yield increase in breeding programs, governed by their hierarchy structure of feature ranking as well as pattern discovery via various combinations of features. PMID:24830330

  15. Land-use history alters contemporary insect herbivore community composition and decouples plant-herbivore relationships.

    SciTech Connect

    Hahn, Philip G.; Orrock, John L.

    2015-04-01

    1. Past land use can create altered soil conditions and plant communities that persist for decades, although the effects of these altered conditions on consumers are rarely investigated. 2. Using a large-scale field study at 36 sites in longleaf pine (Pinus palustris) woodlands, we examined whether historic agricultural land use leads to differences in the abundance and community composition of insect herbivores (grasshoppers, families Acrididae and Tettigoniidae). 3. We measured the cover of six plant functional groups and several environmental variables to determine whether historic agricultural land use affects the relationships between plant cover or environmental conditions and grasshopper assemblages. 4. Land-use history had taxa-specific effects and interacted with herbaceous plant cover to alter grasshopper abundances, leading to significant changes in community composition. Abundance of most grasshopper taxa increased with herbaceous cover in woodlands with no history of agriculture, but there was no relationship in post-agricultural woodlands. We also found that grasshopper abundance was negatively correlated with leaf litter cover. Soil hardness was greater in post-agricultural sites (i.e. more compacted) and was associated with grasshopper community composition. Both herbaceous cover and leaf litter cover are influenced by fire frequency, suggesting a potential indirect role of fire on grasshopper assemblages. 5. Our results demonstrate that historic land use may create persistent differences in the composition of grasshopper assemblages, while contemporary disturbances (e.g. prescribed fire) may be important for determining the abundance of grasshoppers, largely through the effect of fire on plants and leaf litter. Therefore, our results suggest that changes in the contemporary management regimes (e.g. increasing prescribed fire) may not be sufficient to shift the structure of grasshopper communities in post-agricultural sites towards communities in

  16. Technology transfer in agriculture. (Latest citations from the Biobusiness database). Published Search

    SciTech Connect

    1995-02-01

    The bibliography contains citations concerning technology transfer in agriculture. Topics include applications of technology transfer in aquaculture, forestry, soil maintenance, agricultural pollution, agricultural biotechnology, and control of disease and insect pests. Use of computer technology in agriculture and technology transfers to developing countries are discussed. (Contains a minimum of 235 citations and includes a subject term index and title list.)

  17. Technology transfer in agriculture. (Latest citations from the Biobusiness data base). Published Search

    SciTech Connect

    Not Available

    1992-10-01

    The bibliography contains citations concerning technology transfer in agriculture. Topics include applications of technology transfer in aquaculture, forestry, soil maintenance, agricultural pollution, agricultural biotechnology, and control of disease and insect pests. Use of computer technology in agriculture and technology transfers to developing countries are discussed. (Contains a minimum of 178 citations and includes a subject term index and title list.)

  18. Vocational Agriculture Education. Agricultural Mechanics.

    ERIC Educational Resources Information Center

    Smith, Eddie; And Others

    To assist teachers in agricultural mechanics in providing comprehensive instruction to their students, this curriculum guide treats both the mechanical skills and knowlege necessary for this specialized area. Six sections are included, as follow: orientation and safety; agricultural mechanics skills; agricultural power and machinery; agricultural…

  19. Insect bite prevention.

    PubMed

    Moore, Sarah J; Mordue Luntz, Anne Jennifer; Logan, James G

    2012-09-01

    Protection from the bites of arthropod (insect and acarine) vectors of disease is the first line of defense against disease transmission and should be advised in all cases when traveling abroad. Details are described of the main approaches for the prevention of bites, including topical or skin repellents, impregnated clothing, bed nets, and spatial or aerial repellents and aerosols. The bionomics of the main arthropod vectors of disease are described along with photographic plates and tabulated advice to give the traveler. An in-depth treatment of the different protection methodologies provides an up-to-date overview of the technologies involved. PMID:22963776

  20. Department of Agriculture

    MedlinePlus

    ... and Science Rural and Community Development Rural Opportunities Trade Travel and Recreation USDA for Kids Programs and ... and Agriculture Research OPEDA Scholarship Program MARKETING AND TRADE Exporting Goods Importing Goods Newsroom Agency News Releases ...

  1. Aircraft anti-insect system

    NASA Technical Reports Server (NTRS)

    Spiro, Clifford Lawrence (Inventor); Fric, Thomas Frank (Inventor); Leon, Ross Michael (Inventor)

    1997-01-01

    Insect debris is removed from or prevented from adhering to insect impingement areas of an aircraft, particularly on an inlet cowl of an engine, by heating the area to 180.degree.-500.degree. C. An apparatus comprising a means to bring hot air from the aircraft engine to a plenum contiguous to the insect impingement area provides for the heating of the insect impingement areas to the required temperatures. The plenum can include at least one tube with a plurality of holes contained in a cavity within the inlet cowl. It can also include an envelope with a plurality of holes on its surface contained in a cavity within the inlet cowl.

  2. Measuring Asymmetry in Insect-Plant Networks

    NASA Astrophysics Data System (ADS)

    Cruz, Cláudia P. T.; de Almeida, Adriana M.; Corso, Gilberto

    2011-03-01

    In this work we focus on interaction networks between insects and plants and in the characterization of insect plant asymmetry, an important issue in coevolution and evolutionary biology. We analyze in particular the asymmetry in the interaction matrix of animals (herbivorous insects) and plants (food resource for the insects). Instead of driving our attention to the interaction matrix itself we derive two networks associated to the bipartite network: the animal network, D1, and the plant network, D2. These networks are constructed according to the following recipe: two animal species are linked once if they interact with the same plant. In a similar way, in the plant network, two plants are linked if they interact with the same animal. To explore the asymmetry between D2 and D1 we test for a set of 23 networks from the ecologic literature networks: the difference in size, ΔL, clustering coefficient difference, ΔC, and mean connectivity difference, Δ. We used a nonparametric statistical test to check the differences in ΔL, ΔC and Δ. Our results indicate that ΔL and Δ show a significative asymmetry.

  3. 1977 Kansas Field Crop Insect Control Recommendations.

    ERIC Educational Resources Information Center

    Brooks, Leroy; Gates, Dell E.

    This publication is prepared to aid producers in selecting methods of insect population management that have proved effective under Kansas conditions. Topics covered include insect control on alfalfa, soil insects attacking corn, insects attacking above-ground parts of corn, and sorghum, wheat, and soybean insect control. The insecticides…

  4. Importance of rhizobia in Agriculture: potential of the commercial inoculants and native strains for improving legume yields in different land-use systems

    NASA Astrophysics Data System (ADS)

    Lesueur, D.; Atieno, M.; Mathu, S.; Herrmann, L.

    2012-04-01

    Legumes play an important role in the traditional diets of many regions throughout the world because they provide a multitude of benefits to both the soil and other crops grown in combination with them or following them in several cropping systems. The ability of legumes to fix atmospheric nitrogen in association with rhizobia gives them the capacity to grow in very degraded soils. But do we have to systematically inoculate legumes? For example our results suggested that the systematic inoculation of both cowpea and green gram in Kenya with commercial inoculants to improve yields is not really justified, native strains performing better than inoculated strains. But when native rhizobia nodulating legumes are not naturally present, application of rhizobial inoculants is very commonly used. Our results showed that the utilization of effective good-quality rhizobial inoculants by farmers have a real potential to improve legume yields in unfertile soils requesting high applications of mineral fertilizers. For example an effective soybean commercial inoculants was tested in different locations in Kenya (in about 150 farms in 3 mandate areas presenting different soil characteristics and environmental conditions). Application of the rhizobial inoculant significantly increased the soybean yields in all mandate areas (about 75% of the farms). Nodule occupancy analysis showed that a high number of nodules occupied by the inoculated strain did not obviously lead to an increase of soybean production. Soil factors (pH, P, C, N…) seemed to affect the inoculant efficiency whether the strain is occupying the nodules or not. Our statistic analysis showed that soil pH significantly affected nodulation and yield, though the effect was variable depending on the region. We concluded that the competitiveness of rhizobial strains might not be the main factor explaining the effect (or lack of) of legumes inoculation in the field. Another study was aiming to assess if several factors

  5. A novel mechanism of insect resistance engineered into tobacco

    NASA Astrophysics Data System (ADS)

    Hilder, Vaughan A.; Gatehouse, Angharad M. R.; Sheerman, Suzanne E.; Barker, Richard F.; Boulter, Donald

    1987-11-01

    A major goal of plant genetic engineering is the introduction of agronomically desirable phenotypic traits into crop plants in situations where conventional breeding methods have been unsuccessful. One such target is enhanced resistance to insect pests which, in view of the estimated production losses world-wide and the heavy costs of protective treatments, is very important. We report here that a gene encoding a cowpea trypsin inhibitor, which has been shown to give some measure of field resistance to insect pests1, confers, when transferred to tobacco, enhanced resistance to this species' own herbivorous insect pests.

  6. Insect Nicotinic Receptor Agonists as Flea Adulticides in Small Animals

    PubMed Central

    Vo, Dai Tan; Hsu, Walter H.; Martin, Richard J.

    2013-01-01

    Fleas are significant ectoparasites of small animals. They can be a severe irritant to animals and serve as a vector for a number of infectious diseases. In this article, we discuss the pharmacological characteristics of four insect nicotinic acetylcholine receptor (nAChR) agonists used as fleacides in dogs and cats, which include three neonicotinoids (imidacloprid, nitenpyram, and dinotefuran) and spinosad. Insect nAChR agonists are one of the most important new classes of insecticides, which are used to control sucking insects both on plants and on companion animals. These new compounds provide a new approach for practitioners to safely and effectively eliminate fleas. PMID:20646191

  7. Virtual bats: manipulating the activities of bats and insects as a practical application for integrated pest control

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Many insects, including many agricultural pests, respond to the ultrasonic calls of bats by taking evasive action and curtailing flight activity. Earlier attempts to exploit this co-evolved relationship to inhibit insect activity for crop protection (Belton and Kempster 1962) showed promise, but we...

  8. Agriculture Supplies & Services. Volume 1 of 3.

    ERIC Educational Resources Information Center

    Kansas State Univ., Manhattan.

    The first of three volumes included in a secondary agricultural supplies and services curriculum guide, this volume contains units of instruction in two major areas: (1) plant and soil science and (2) leadership (Future Farmers of America). Typical of the nineteen units included in the first section are the following: Plant Insect Control, Plant…

  9. Plant volatile analogues strengthen attractiveness to insect.

    PubMed

    Sun, Yufeng; Yu, Hao; Zhou, Jing-Jiang; Pickett, John A; Wu, Kongming

    2014-01-01

    Green leaf bug Apolygus lucorum (Meyer-Dür) is one of the major pests in agriculture. Management of A. lucorum was largely achieved by using pesticides. However, the increasing population of A. lucorum since growing Bt cotton widely and the increased awareness of ecoenvironment and agricultural product safety makes their population-control very challenging. Therefore this study was conducted to explore a novel ecological approach, synthetic plant volatile analogues, to manage the pest. Here, plant volatile analogues were first designed and synthesized by combining the bioactive components of β-ionone and benzaldehyde. The stabilities of β-ionone, benzaldehyde and analogue 3 g were tested. The electroantennogram (EAG) responses of A. lucorum adult antennae to the analogues were recorded. And the behavior assay and filed experiment were also conducted. In this study, thirteen analogues were acquired. The analogue 3 g was demonstrated to be more stable than β-ionone and benzaldehyde in the environment. Many of the analogues elicited EAG responses, and the EAG response values to 3 g remained unchanged during seven-day period. 3 g was also demonstrated to be attractive to A. lucorum adults in the laboratory behavior experiment and in the field. Its attractiveness persisted longer than β-ionone and benzaldehyde. This indicated that 3 g can strengthen attractiveness to insect and has potential as an attractant. Our results suggest that synthetic plant volatile analogues can strengthen attractiveness to insect. This is the first published study about synthetic plant volatile analogues that have the potential to be used in pest control. Our results will support a new ecological approach to pest control and it will be helpful to ecoenvironment and agricultural product safety. PMID:24911460

  10. Plant Volatile Analogues Strengthen Attractiveness to Insect

    PubMed Central

    Sun, Yufeng; Yu, Hao; Zhou, Jing-Jiang; Pickett, John A.; Wu, Kongming

    2014-01-01

    Green leaf bug Apolygus lucorum (Meyer-Dür) is one of the major pests in agriculture. Management of A. lucorum was largely achieved by using pesticides. However, the increasing population of A. lucorum since growing Bt cotton widely and the increased awareness of ecoenvironment and agricultural product safety makes their population-control very challenging. Therefore this study was conducted to explore a novel ecological approach, synthetic plant volatile analogues, to manage the pest. Here, plant volatile analogues were first designed and synthesized by combining the bioactive components of β-ionone and benzaldehyde. The stabilities of β-ionone, benzaldehyde and analogue 3 g were tested. The electroantennogram (EAG) responses of A. lucorum adult antennae to the analogues were recorded. And the behavior assay and filed experiment were also conducted. In this study, thirteen analogues were acquired. The analogue 3 g was demonstrated to be more stable than β-ionone and benzaldehyde in the environment. Many of the analogues elicited EAG responses, and the EAG response values to 3 g remained unchanged during seven-day period. 3 g was also demonstrated to be attractive to A. lucorum adults in the laboratory behavior experiment and in the field. Its attractiveness persisted longer than β-ionone and benzaldehyde. This indicated that 3 g can strengthen attractiveness to insect and has potential as an attractant. Our results suggest that synthetic plant volatile analogues can strengthen attractiveness to insect. This is the first published study about synthetic plant volatile analogues that have the potential to be used in pest control. Our results will support a new ecological approach to pest control and it will be helpful to ecoenvironment and agricultural product safety. PMID:24911460

  11. Agriculture, summary

    NASA Technical Reports Server (NTRS)

    Baldwin, R.

    1975-01-01

    Applications of remotely sensed data in agriculture are enumerated. These include: predictions of forage for range animal consumption, forest management, soil mapping, and crop inventory and management.

  12. Plant Defense against Insect Herbivores

    PubMed Central

    Fürstenberg-Hägg, Joel; Zagrobelny, Mika; Bak, Søren

    2013-01-01

    Plants have been interacting with insects for several hundred million years, leading to complex defense approaches against various insect feeding strategies. Some defenses are constitutive while others are induced, although the insecticidal defense compound or protein classes are often similar. Insect herbivory induce several internal signals from the wounded tissues, including calcium ion fluxes, phosphorylation cascades and systemic- and jasmonate signaling. These are perceived in undamaged tissues, which thereafter reinforce their defense by producing different, mostly low molecular weight, defense compounds. These bioactive specialized plant defense compounds may repel or intoxicate insects, while defense proteins often interfere with their digestion. Volatiles are released upon herbivory to repel herbivores, attract predators or for communication between leaves or plants, and to induce defense responses. Plants also apply morphological features like waxes, trichomes and latices to make the feeding more difficult for the insects. Extrafloral nectar, food bodies and nesting or refuge sites are produced to accommodate and feed the predators of the herbivores. Meanwhile, herbivorous insects have adapted to resist plant defenses, and in some cases even sequester the compounds and reuse them in their own defense. Both plant defense and insect adaptation involve metabolic costs, so most plant-insect interactions reach a stand-off, where both host and herbivore survive although their development is suboptimal. PMID:23681010

  13. Polarization Imaging and Insect Vision

    ERIC Educational Resources Information Center

    Green, Adam S.; Ohmann, Paul R.; Leininger, Nick E.; Kavanaugh, James A.

    2010-01-01

    For several years we have included discussions about insect vision in the optics units of our introductory physics courses. This topic is a natural extension of demonstrations involving Brewster's reflection and Rayleigh scattering of polarized light because many insects heavily rely on optical polarization for navigation and communication.…

  14. Reader Survey for INSECT ALERTS.

    ERIC Educational Resources Information Center

    Miller, Mason E.; Sauer, Richard J.

    To determine what might be done to improve "Insect Alerts," which is a newsletter that carries "information on insect biology, abundance, activity and interpretation of control need," put out through the Michigan Cooperative Extension Service 26 weeks a year, a survey was conducted. A mail questionnaire was sent to all 120 county extension…

  15. RNAI: Future in insect management

    Technology Transfer Automated Retrieval System (TEKTRAN)

    RNA interference is a post-transcriptional, gene regulation mechanism found in virtually all plants and animals including insects. The demonstration of RNAi in insects and its successful use as a tool in the study of functional genomics opened the door to the development of a variety of novel, envir...

  16. Chickpea Ascochyta blight and insects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Early symptoms of Acochyta blight and insect damages were detected in the Paliuse region.This article informs chickpea scientists and growers about current disease and insect pest problems in the Palouse region. Ascochyta blight appeared in many chickpea fields and was severe in some fields. Insec...

  17. A Template for Insect Cryopreservation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This article is intended to update the reader on the progress made on insect embryo cryopreservation in the past 20 years and gives information for developing a protocol for cryopreserving insects by using a 2001 study as a template. The study used for the template is the cryopreservation of the Old...

  18. Eicosanoids mediate insect hemocyte migration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Hemocyte chemotaxis toward infection and wound sites is an essential component of insect defense reactions, although the biochemical signal mechanisms responsible for mediating chemotaxis in insect cells are not well understood. Here we report on the outcomes of experiments designed to test the hyp...

  19. Similarities between decapod and insect neuropeptidomes

    PubMed Central

    2016-01-01

    Background. Neuropeptides are important regulators of physiological processes and behavior. Although they tend to be generally well conserved, recent results using trancriptome sequencing on decapod crustaceans give the impression of significant differences between species, raising the question whether such differences are real or artefacts. Methods. The BLAST+ program was used to find short reads coding neuropeptides and neurohormons in publicly available short read archives. Such reads were then used to find similar reads in the same archives, and the DNA assembly program Trinity was employed to construct contigs encoding the neuropeptide precursors as completely as possible. Results. The seven decapod species analyzed in this fashion, the crabs Eriocheir sinensis, Carcinus maenas and Scylla paramamosain, the shrimp Litopenaeus vannamei, the lobster Homarus americanus, the fresh water prawn Macrobrachium rosenbergii and the crayfish Procambarus clarkii had remarkably similar neuropeptidomes. Although some neuropeptide precursors could not be assembled, in many cases individual reads pertaining to the missing precursors show unambiguously that these neuropeptides are present in these species. In other cases, the tissues that express those neuropeptides were not used in the construction of the cDNA libraries. One novel neuropeptide was identified: elongated PDH (pigment dispersing hormone), a variation on PDH that has a two-amino-acid insertion in its core sequence. Hyrg is another peptide that is ubiquitously present in decapods and is likely a novel neuropeptide precursor. Discussion. Many insect species have lost one or more neuropeptide genes, but apart from elongated PDH and hyrg all other decapod neuropeptides are present in at least some insect species, and allatotropin is the only insect neuropeptide missing from decapods. This strong similarity between insect and decapod neuropeptidomes makes it possible to predict the receptors for decapod neuropeptides

  20. Similarities between decapod and insect neuropeptidomes.

    PubMed

    Veenstra, Jan A

    2016-01-01

    Background. Neuropeptides are important regulators of physiological processes and behavior. Although they tend to be generally well conserved, recent results using trancriptome sequencing on decapod crustaceans give the impression of significant differences between species, raising the question whether such differences are real or artefacts. Methods. The BLAST+ program was used to find short reads coding neuropeptides and neurohormons in publicly available short read archives. Such reads were then used to find similar reads in the same archives, and the DNA assembly program Trinity was employed to construct contigs encoding the neuropeptide precursors as completely as possible. Results. The seven decapod species analyzed in this fashion, the crabs Eriocheir sinensis, Carcinus maenas and Scylla paramamosain, the shrimp Litopenaeus vannamei, the lobster Homarus americanus, the fresh water prawn Macrobrachium rosenbergii and the crayfish Procambarus clarkii had remarkably similar neuropeptidomes. Although some neuropeptide precursors could not be assembled, in many cases individual reads pertaining to the missing precursors show unambiguously that these neuropeptides are present in these species. In other cases, the tissues that express those neuropeptides were not used in the construction of the cDNA libraries. One novel neuropeptide was identified: elongated PDH (pigment dispersing hormone), a variation on PDH that has a two-amino-acid insertion in its core sequence. Hyrg is another peptide that is ubiquitously present in decapods and is likely a novel neuropeptide precursor. Discussion. Many insect species have lost one or more neuropeptide genes, but apart from elongated PDH and hyrg all other decapod neuropeptides are present in at least some insect species, and allatotropin is the only insect neuropeptide missing from decapods. This strong similarity between insect and decapod neuropeptidomes makes it possible to predict the receptors for decapod neuropeptides

  1. Agricultural pest monitoring using fluorescence lidar techniques. Feasibility study

    NASA Astrophysics Data System (ADS)

    Mei, L.; Guan, Z. G.; Zhou, H. J.; Lv, J.; Zhu, Z. R.; Cheng, J. A.; Chen, F. J.; Löfstedt, C.; Svanberg, S.; Somesfalean, G.

    2012-03-01

    The fluorescence of different types of planthopper ( Hemiptera) and moth ( Lepidoptera), which constitute important Chinese agricultural pests, was investigated both in situ in a laboratory setting and remotely using a fluorescence light detection and ranging (lidar) system operating at a range of about 50 m. The natural autofluorescence of different species, as well as the fluorescence from insects that had been dusted with fluorescent dye powder for identification were studied. Autofluorescence spectra of both moths and planthoppers show a maximum intensity peak around 450 nm. Bleaching upon long-time laser illumination was modest and did not affect the shape of the spectrum. A single dyed rice planthopper, a few mm in size, could be detected at 50 m distance by using the fluorescence lidar system. By employing various marking dyes, different types of agricultural pest could be determined. We suggest that lidar may be used in studies of migration and movement of pest insects, including studies of their behavior in the vicinity of pheromone traps and in pheromone-treated fields.

  2. Insect overwintering in a changing climate.

    PubMed

    Bale, J S; Hayward, S A L

    2010-03-15

    Insects are highly successful animals inhabiting marine, freshwater and terrestrial habitats from the equator to the poles. As a group, insects have limited ability to regulate their body temperature and have thus required a range of strategies to support life in thermally stressful environments, including behavioural avoidance through migration and seasonal changes in cold tolerance. With respect to overwintering strategies, insects have traditionally been divided into two main groups: freeze tolerant and freeze avoiding, although this simple classification is underpinned by a complex of interacting processes, i.e. synthesis of ice nucleating agents, cryoprotectants, antifreeze proteins and changes in membrane lipid composition. Also, in temperate and colder climates, the overwintering ability of many species is closely linked to the diapause state, which often increases cold tolerance ahead of temperature-induced seasonal acclimatisation. Importantly, even though most species can invoke one or both of these responses, the majority of insects die from the effects of cold rather than freezing. Most studies on the effects of a changing climate on insects have focused on processes that occur predominantly in summer (development, reproduction) and on changes in distributions rather than winter survival per se. For species that routinely experience cold stress, a general hypothesis would be that predicted temperature increases of 1 degree C to 5 degrees C over the next 50-100 years would increase winter survival in some climatic zones. However, this is unlikely to be a universal effect. Negative impacts may occur if climate warming leads to a reduction or loss of winter snow cover in polar and sub-polar areas, resulting in exposure to more severe air temperatures, increasing frequency of freeze-thaw cycles and risks of ice encasement. Likewise, whilst the dominant diapause-inducing cue (photoperiod) will be unaffected by global climate change, higher temperatures may

  3. Insect Immunity to Entomopathogenic Fungi.

    PubMed

    Lu, H-L; St Leger, R J

    2016-01-01

    The study of infection and immunity in insects has achieved considerable prominence with the appreciation that their host defense mechanisms share many fundamental characteristics with the innate immune system of vertebrates. Studies on the highly tractable model organism Drosophila in particular have led to a detailed understanding of conserved innate immunity networks, such as Toll. However, most of these studies have used opportunistic human pathogens and may not have revealed specialized immune strategies that have arisen through evolutionary arms races with natural insect pathogens. Fungi are the commonest natural insect pathogens, and in this review, we focus on studies using Metarhizium and Beauveria spp. that have addressed immune system function and pathogen virulence via behavioral avoidance, the use of physical barriers, and the activation of local and systemic immune responses. In particular, we highlight studies on the evolutionary genetics of insect immunity and discuss insect-pathogen coevolution. PMID:27131327

  4. Population fluctuation in phytophagous insects

    SciTech Connect

    Redfearn, A.; Pimm, S.L. )

    1994-06-01

    We examined how community interactions affect year-to-year population variability in three groups of phytophagous insects: British aphids and moths, and Canadian moths. We first examined how the number of host plant species on which a given phytophagous insect species feeds affects its population variability. Specialist insect species showed a weak tendency to be more variable than generalist species. We then examined how the number of species of parasitoids from which a given phytophagous insects species suffers affects its population variability. Species that are host to few parasitoid species showed a weak tendency to be more variable than species with many parsitoid species. These relationships also depend on other aspects of the life histories of the phytophagous insect species.

  5. Optoelectronic determination of insect presence in fruit

    NASA Astrophysics Data System (ADS)

    Shrestha, Bim P.; Guyer, Daniel E.; Ariana, Diwan P.

    2004-03-01

    Opto-electronic methods represent a potential to identify the presence of insect activities on or within agricultural commodities. Such measurements may detect actual insect presence or indirect secondary changes in the product resulting from past or present insect activities. Preliminary imaging studies have demonstrated some unique spectral characteristics of insect larvae on cherries. A detailed study on spectral characteristics of healthy and infested tart cherry tissue with and without larvae (Plum Curculio) was conducted for reflectance, transmittance and interactance modes for each of UV and visible/NIR light sources. The intensity of transmitted UV signals through the tart cherry was found to be weak; however, the spectral properties of UV light in reflectance mode has revealed some typical characteristics of larvae on healthy and infested tissue. The larvae on tissue were found to exhibit UV induced fluorescence signals in the range of 400-700 nm. Multi spectral imaging of the halved tart cherry has also corroborated this particular behavior of plum curculio larvae. The gray scale subtraction between corresponding pixels in these multi-spectral images has helped to locate the larvae precisely on the tart cherry tissue background, which otherwise was inseparable. The spectral characteristics of visible/NIR energy in transmittance and reflectance mode are capable of estimating the secondary effect of infestation in tart cherry tissue. The study has shown the shifting in peaks of reflected and transmitted signals from healthy and infested tissues and coincides with the concept of browning of tissue at cell level as a process of infestation. Interactance study has been carried out to study the possibility of coupling opto-electronic devices with the existing pitting process. The shifting of peaks has been observed for the normalized intensity of healthy and infested tissues. The study has been able to establish the inherent spectral characteristic of these

  6. Grooming Behavior as a Mechanism of Insect Disease Defense

    PubMed Central

    Zhukovskaya, Marianna; Yanagawa, Aya; Forschler, Brian T.

    2013-01-01

    Grooming is a well-recognized, multipurpose, behavior in arthropods and vertebrates. In this paper, we review the literature to highlight the physical function, neurophysiological mechanisms, and role that grooming plays in insect defense against pathogenic infection. The intricate relationships between the physical, neurological and immunological mechanisms of grooming are discussed to illustrate the importance of this behavior when examining the ecology of insect-pathogen interactions. PMID:26462526

  7. The aerodynamics of insect flight.

    PubMed

    Sane, Sanjay P

    2003-12-01

    The flight of insects has fascinated physicists and biologists for more than a century. Yet, until recently, researchers were unable to rigorously quantify the complex wing motions of flapping insects or measure the forces and flows around their wings. However, recent developments in high-speed videography and tools for computational and mechanical modeling have allowed researchers to make rapid progress in advancing our understanding of insect flight. These mechanical and computational fluid dynamic models, combined with modern flow visualization techniques, have revealed that the fluid dynamic phenomena underlying flapping flight are different from those of non-flapping, 2-D wings on which most previous models were based. In particular, even at high angles of attack, a prominent leading edge vortex remains stably attached on the insect wing and does not shed into an unsteady wake, as would be expected from non-flapping 2-D wings. Its presence greatly enhances the forces generated by the wing, thus enabling insects to hover or maneuver. In addition, flight forces are further enhanced by other mechanisms acting during changes in angle of attack, especially at stroke reversal, the mutual interaction of the two wings at dorsal stroke reversal or wing-wake interactions following stroke reversal. This progress has enabled the development of simple analytical and empirical models that allow us to calculate the instantaneous forces on flapping insect wings more accurately than was previously possible. It also promises to foster new and exciting multi-disciplinary collaborations between physicists who seek to explain the phenomenology, biologists who seek to understand its relevance to insect physiology and evolution, and engineers who are inspired to build micro-robotic insects using these principles. This review covers the basic physical principles underlying flapping flight in insects, results of recent experiments concerning the aerodynamics of insect flight, as well

  8. Insect attraction to herbivore-induced beech volatiles under different forest management regimes.

    PubMed

    Gossner, Martin M; Weisser, Wolfgang W; Gershenzon, Jonathan; Unsicker, Sybille B

    2014-10-01

    Insect herbivore enemies such as parasitoids and predators are important in controlling herbivore pests. From agricultural systems we know that land-use intensification can negatively impact biological control as an important ecosystem service. The aim of our study was to investigate the importance of management regime for natural enemy pressure and biological control possibilities in forests dominated by European beech. We hypothesize that the volatile blend released from herbivore-infested beech trees functions as a signal, attracting parasitoids and herbivore enemies. Furthermore, we hypothesize that forest management regime influences the composition of species attracted by these herbivore-induced beech volatiles. We installed flight-interception traps next to Lymantria dispar caterpillar-infested young beech trees releasing herbivore-induced volatiles and next to non-infested control trees. Significantly more parasitoids were captured next to caterpillar-infested trees compared to non-infested controls, irrespective of forest type. However, the composition of the trophic guilds in the traps did vary in response to forest management regime. While the proportion of chewing insects was highest in non-managed forests, the proportion of sucking insects peaked in forests with low management and of parasitoids in young, highly managed, forest stands. Neither the number of naturally occurring beech saplings nor herbivory levels in the proximity of our experiment affected the abundance and diversity of parasitoids caught. Our data show that herbivore-induced beech volatiles attract herbivore enemies under field conditions. They further suggest that differences in the structural complexity of forests as a consequence of management regime only play a minor role in parasitoid activity and thus in indirect tree defense. PMID:25080178

  9. Debris-carrying camouflage among diverse lineages of Cretaceous insects.

    PubMed

    Wang, Bo; Xia, Fangyuan; Engel, Michael S; Perrichot, Vincent; Shi, Gongle; Zhang, Haichun; Chen, Jun; Jarzembowski, Edmund A; Wappler, Torsten; Rust, Jes

    2016-06-01

    Insects have evolved diverse methods of camouflage that have played an important role in their evolutionary success. Debris-carrying, a behavior of actively harvesting and carrying exogenous materials, is among the most fascinating and complex behaviors because it requires not only an ability to recognize, collect, and carry materials but also evolutionary adaptations in related morphological characteristics. However, the fossil record of such behavior is extremely scarce, and only a single Mesozoic example from Spanish amber has been recorded; therefore, little is known about the early evolution of this complicated behavior and its underlying anatomy. We report a diverse insect assemblage of exceptionally preserved debris carriers from Cretaceous Burmese, French, and Lebanese ambers, including the earliest known chrysopoid larvae (green lacewings), myrmeleontoid larvae (split-footed lacewings and owlflies), and reduviids (assassin bugs). These ancient insects used a variety of debris material, including insect exoskeletons, sand grains, soil dust, leaf trichomes of gleicheniacean ferns, wood fibers, and other vegetal debris. They convergently evolved their debris-carrying behavior through multiple pathways, which expressed a high degree of evolutionary plasticity. We demonstrate that the behavioral repertoire, which is associated with considerable morphological adaptations, was already widespread among insects by at least the Mid-Cretaceous. Together with the previously known Spanish specimen, these fossils are the oldest direct evidence of camouflaging behavior in the fossil record. Our findings provide a novel insight into early evolution of camouflage in insects and ancient ecological associations among plants and insects. PMID:27386568

  10. A Review of Chemosensation and Related Behavior in Aquatic Insects

    PubMed Central

    Crespo, José G.

    2011-01-01

    Insects that are secondarily adapted to aquatic environments are able to sense odors from a diverse array of sources. The antenna of these insects, as in all insects, is the main chemosensory structure and its input to the brain allows for integration of sensory information that ultimately ends in behavioral responses. Only a fraction of the aquatic insect orders have been studied with respect to their sensory biology and most of the work has centered either on the description of the different types of sensilla, or on the behavior of the insect as a whole. In this paper, the literature is exhaustively reviewed and ways in which antennal morphology, brain structure, and associated behavior can advance better understanding of the neurobiology involved in processing of chemosensory information are discussed. Moreover, the importance of studying such group of insects is stated, and at the same time it is shown that many interesting questions regarding olfactory processing can be addressed by looking into the changes that aquatic insects undergo when leaving their aquatic environment. PMID:21864156

  11. Molecular Genetics of Beauveria bassiana Infection of Insects.

    PubMed

    Ortiz-Urquiza, A; Keyhani, N O

    2016-01-01

    Research on the insect pathogenic filamentous fungus, Beauveria bassiana has witnessed significant growth in recent years from mainly physiological studies related to its insect biological control potential, to addressing fundamental questions regarding the underlying molecular mechanisms of fungal development and virulence. This has been in part due to a confluence of robust genetic tools and genomic resources for the fungus, and recognition of expanded ecological interactions with which the fungus engages. Beauveria bassiana is a broad host range insect pathogen that has the ability to form intimate symbiotic relationships with plants. Indeed, there is an increasing realization that the latter may be the predominant environmental interaction in which the fungus participates, and that insect parasitism may be an opportunist lifestyle evolved due to the carbon- and nitrogen-rich resources present in insect bodies. Here, we will review progress on the molecular genetics of B. bassiana, which has largely been directed toward identifying genetic pathways involved in stress response and virulence assumed to have practical applications in improving the insect control potential of the fungus. Important strides have also been made in understanding aspects of B. bassiana development. Finally, although increasingly apparent in a number of studies, there is a need for progressing beyond phenotypic mutant characterization to sufficiently investigate the molecular mechanisms underlying B. bassiana's unique and diverse lifestyles as saprophyte, insect pathogen, and plant mutualist. PMID:27131326

  12. Debris-carrying camouflage among diverse lineages of Cretaceous insects

    PubMed Central

    Wang, Bo; Xia, Fangyuan; Engel, Michael S.; Perrichot, Vincent; Shi, Gongle; Zhang, Haichun; Chen, Jun; Jarzembowski, Edmund A.; Wappler, Torsten; Rust, Jes

    2016-01-01

    Insects have evolved diverse methods of camouflage that have played an important role in their evolutionary success. Debris-carrying, a behavior of actively harvesting and carrying exogenous materials, is among the most fascinating and complex behaviors because it requires not only an ability to recognize, collect, and carry materials but also evolutionary adaptations in related morphological characteristics. However, the fossil record of such behavior is extremely scarce, and only a single Mesozoic example from Spanish amber has been recorded; therefore, little is known about the early evolution of this complicated behavior and its underlying anatomy. We report a diverse insect assemblage of exceptionally preserved debris carriers from Cretaceous Burmese, French, and Lebanese ambers, including the earliest known chrysopoid larvae (green lacewings), myrmeleontoid larvae (split-footed lacewings and owlflies), and reduviids (assassin bugs). These ancient insects used a variety of debris material, including insect exoskeletons, sand grains, soil dust, leaf trichomes of gleicheniacean ferns, wood fibers, and other vegetal debris. They convergently evolved their debris-carrying behavior through multiple pathways, which expressed a high degree of evolutionary plasticity. We demonstrate that the behavioral repertoire, which is associated with considerable morphological adaptations, was already widespread among insects by at least the Mid-Cretaceous. Together with the previously known Spanish specimen, these fossils are the oldest direct evidence of camouflaging behavior in the fossil record. Our findings provide a novel insight into early evolution of camouflage in insects and ancient ecological associations among plants and insects. PMID:27386568

  13. Agricultural Wastes.

    ERIC Educational Resources Information Center

    Jewell, W. J.; Switzenbaum, M. S.

    1978-01-01

    Presents a literature review of agricultural wastes, covering publications of 1976-77. Some of the areas covered are: (1) water characteristics and impacts; (2) waste treatment; (3) reuse of agricultural wastes; and (4) nonpoint pollution sources. A list of 150 references is also presented. (HM)

  14. VOCATIONAL AGRICULTURE.

    ERIC Educational Resources Information Center

    California State Dept. of Education, Sacramento. Research Coordinating Unit.

    TO ASSIST THOSE WHO MAKE DECISIONS RELATING TO EDUCATIONAL PROGRAMS IN AGRICULTURE, RECENT RESEARCH IN VOCATIONAL AGRICULTURE IS SUMMARIZED. A 1963 STUDY TREATS THE RELATIONSHIP BETWEEN WORK EXPERIENCE AND STUDENT CHARACTERISTICS, PLANS, AND ASPIRATIONS. STUDIES ON POST-SECONDARY EDUCATION CONCERN GUIDELINES FOR TECHNICIAN PROGRAMS, JUSTIFICATION…

  15. The Agricultural Educator's Role in Helping Elementary Pupils Learn About Agriculture

    ERIC Educational Resources Information Center

    Swan, Malcolm; Donaldson, George W.

    1970-01-01

    Agriculture should occupy an important place in the curriculum of the schools. Children should be provided experiences that enable development of an understanding and appreciation of agriculture. (DM)

  16. Fluorescent in situ hybridization for the localization of viruses, bacteria and other microorganisms in insect and plant tissues.

    PubMed

    Kliot, Adi; Ghanim, Murad

    2016-04-01

    Methods for the localization of cellular components such as nucleic acids, proteins, cellular vesicles and more, and the localization of microorganisms including viruses, bacteria and fungi have become an important part of any research program in biological sciences that enable the visualization of these components in fixed and live tissues without the need for complex processing steps. The rapid development of microscopy tools and technologies as well as related fluorescent markers and fluorophores for many cellular components, and the ability to design DNA and RNA sequence-based molecular probes and antibodies which can be visualized fluorescently, have rapidly advanced this field. This review will focus on some of the localizations methods which have been used in plants and insect pests in agriculture, and other microorganisms, which are rapidly advancing the research in agriculture-related fields. PMID:26678796

  17. Velocity correlations in laboratory insect swarms

    NASA Astrophysics Data System (ADS)

    Ni, R.; Ouellette, N. T.

    2015-12-01

    In contrast to animal groups such as bird flocks or migratory herds that display net, directed motion, insect swarms do not possess global order. Without such order, it is difficult to define and characterize the transition to collective behavior in swarms; nevertheless, visual observation of swarms strongly suggests that swarming insects do behave collectively. It has recently been suggested that correlation rather than order is the hallmark of emergent collective behavior. Here, we report measurements of spatial velocity correlation functions in laboratory mating swarms of the non-biting midge Chironomus riparius. Although we find some correlation at short distances, our swarms are in general only weakly correlated, in contrast to what has been observed in field studies. Our results hint at the potentially important role of environmental conditions on collective behavior, and suggest that general indicators of the collective nature of swarming are still needed.

  18. Sterile-Insect Methods for Control of Mosquito-Borne Diseases: An Analysis

    PubMed Central

    Benedict, Mark; Bellini, Romeo; Clark, Gary G.; Dame, David A.; Service, Mike W.; Dobson, Stephen L.

    2010-01-01

    Abstract Effective vector control, and more specifically mosquito control, is a complex and difficult problem, as illustrated by the continuing prevalence (and spread) of mosquito-transmitted diseases. The sterile insect technique and similar methods control certain agricultural insect pest populations in a species-specific, environmentally sound, and effective manner; there is increased interest in applying this approach to vector control. Such an approach, like all others in use and development, is not a one-size-fits-all solution, and will be more appropriate in some situations than others. In addition, the proposed release of pest insects, and more so genetically modified pest insects, is bound to raise questions in the general public and the scientific community as to such a method's efficacy, safety, and sustainability. This article attempts to address these concerns and indicate where sterile-insect methods are likely to be useful for vector control. PMID:19725763

  19. Sterile-insect methods for control of mosquito-borne diseases: an analysis.

    PubMed

    Alphey, Luke; Benedict, Mark; Bellini, Romeo; Clark, Gary G; Dame, David A; Service, Mike W; Dobson, Stephen L

    2010-04-01

    Effective vector control, and more specifically mosquito control, is a complex and difficult problem, as illustrated by the continuing prevalence (and spread) of mosquito-transmitted diseases. The sterile insect technique and similar methods control certain agricultural insect pest populations in a species-specific, environmentally sound, and effective manner; there is increased interest in applying this approach to vector control. Such an approach, like all others in use and development, is not a one-size-fits-all solution, and will be more appropriate in some situations than others. In addition, the proposed release of pest insects, and more so genetically modified pest insects, is bound to raise questions in the general public and the scientific community as to such a method's efficacy, safety, and sustainability. This article attempts to address these concerns and indicate where sterile-insect methods are likely to be useful for vector control. PMID:19725763

  20. [Use of agricultural insecticides in Benin].

    PubMed

    Akogbeto, M C; Djouaka, R; Noukpo, H

    2005-12-01

    The use of insecticides in households and in agriculture has been incriminated in the emergence of insecticide resistance in insect vectors. For farming staff, the emergence of vector resistance is due to indoors spray of insecticides using aerosols and other low quality products in rural and urban settings against mosquitoes. On the other hand, public health specialists believe that the phenomenon of resistance could be due to massive use of insecticides in agriculture for field pests control. In Turkey, the implication of agricultural use of pesticides in the selection of vector resistance is clearly established. This study was framed to identify potential practices favouring the emergence of insecticide resistance in the Republic of Benin. Interviews and focus group discussions were organized with cotton, rice and vegetables farmers. The final aim of these surveys was to point out practices likely to favour the emergence of resistance. The research is conducted in 3 cotton fields, 2 rice fields and 2 vegetable plantations. After filling and signing concerned forms, farmers are subjected to quantitative and qualitative questionnaires to generate data on: insecticides being used, the various doses applied for pests eradication, the frequency of treatments, the cost of treatments (cost/hectare/year) the origin of insecticides, the place of purchase, safety precautions and related health hazards. The results of this study have shown that the use of insecticides in agriculture is a clear fact. During treatments, insecticide residues get in contact with mosquito breeding sites where they diffuse into water and exercise a selection pressure on larvae. This partially explains the high levels of resistance recorded in with strains of Anopheles gambiae collected in agricultural settings under insecticides pressure. Pyrethroids and more specifically deltamethrin and cyfluthrin are the insecticides mainly used in studied localities. Bedrooms of farmers are used as storage

  1. Habitat Re-Creation (Ecological Restoration) as a Strategy for Conserving Insect Communities in Highly Fragmented Landscapes

    PubMed Central

    Shuey, John A.

    2013-01-01

    Because of their vast diversity, measured by both numbers of species as well as life history traits, insects defy comprehensive conservation planning. Thus, almost all insect conservation efforts target individual species. However, serious insect conservation requires goals that are set at the faunal level and conservation success requires strategies that conserve intact communities. This task is complicated in agricultural landscapes by high levels of habitat fragmentation and isolation. In many regions, once widespread insect communities are now functionally trapped on islands of ecosystem remnants and subject to a variety of stressors associated with isolation, small population sizes and artificial population fragmentation. In fragmented landscapes ecological restoration can be an effective strategy for reducing localized insect extinction rates, but insects are seldom included in restoration design criteria. It is possible to incorporate a few simple conservation criteria into restoration designs that enhance impacts to entire insect communities. Restoration can be used as a strategy to address fragmentation threats to isolated insect communities if insect communities are incorporated at the onset of restoration planning. Fully incorporating insect communities into restoration designs may increase the cost of restoration two- to three-fold, but the benefits to biodiversity conservation and the ecological services provided by intact insect communities justify the cost. PMID:26462535

  2. Innovative applications for insect viruses: towards insecticide sensitization.

    PubMed

    Lapied, Bruno; Pennetier, Cédric; Apaire-Marchais, Véronique; Licznar, Patricia; Corbel, Vincent

    2009-04-01

    The effective management of emerging insect-borne disease is dependent on the use of safe and efficacious chemical insecticides. Given the inherent ability of insects to develop resistance, it is essential to propose innovative strategies because insecticides remain the most important element of integrated approaches to vector control. Recently, intracellular phosphorylation and dephosphorylation of membrane receptors and ion channels targeted by insecticides have been described as new processes for increasing the sensitivity of insecticides. An efficient method might be to infect host insects with recombinant viruses overexpressing specific protein phosphatases/kinases known to regulate specific insecticide-sensitive targets. This attractive strategy could lead to sensitization of the insects, thus reducing the doses of insecticides and increasing the efficacy of treatments. PMID:19251330

  3. Insect antiviral innate immunity: pathways, effectors, and connections.

    PubMed

    Kingsolver, Megan B; Huang, Zhijing; Hardy, Richard W

    2013-12-13

    Insects are infected by a wide array of viruses some of which are insect restricted and pathogenic, and some of which are transmitted by biting insects to vertebrates. The medical and economic importance of these viruses heightens the need to understand the interaction between the infecting pathogen and the insect immune system in order to develop transmission interventions. The interaction of the virus with the insect host innate immune system plays a critical role in the outcome of infection. The major mechanism of antiviral defense is the small, interfering RNA pathway that responds through the detection of virus-derived double-stranded RNA to suppress virus replication. However, other innate antimicrobial pathways such as Imd, Toll, and Jak-STAT and the autophagy pathway have also been shown to play important roles in antiviral immunity. In this review, we provide an overview of the current understanding of the main insect antiviral pathways and examine recent findings that further our understanding of the roles of these pathways in facilitating a systemic and specific response to infecting viruses. PMID:24120681

  4. Ultraviolet safety assessments of insect light traps.

    PubMed

    Sliney, David H; Gilbert, David W; Lyon, Terry

    2016-06-01

    Near-ultraviolet (UV-A: 315-400 nm), "black-light," electric lamps were invented in 1935 and ultraviolet insect light traps (ILTs) were introduced for use in agriculture around that time. Today ILTs are used indoors in several industries and in food-service as well as in outdoor settings. With recent interest in photobiological lamp safety, safety standards are being developed to test for potentially hazardous ultraviolet emissions. A variety of UV "Black-light" ILTs were measured at a range of distances to assess potential exposures. Realistic time-weighted human exposures are shown to be well below current guidelines for human exposure to ultraviolet radiation. These UV-A exposures would be far less than the typical UV-A exposure in the outdoor environment. Proposals are made for realistic ultraviolet safety standards for ILT products. PMID:27043058

  5. Ultraviolet safety assessments of insect light traps

    PubMed Central

    Sliney, David H.; Gilbert, David W.; Lyon, Terry

    2016-01-01

    ABSTRACT Near-ultraviolet (UV-A: 315–400 nm), “black-light,” electric lamps were invented in 1935 and ultraviolet insect light traps (ILTs) were introduced for use in agriculture around that time. Today ILTs are used indoors in several industries and in food-service as well as in outdoor settings. With recent interest in photobiological lamp safety, safety standards are being developed to test for potentially hazardous ultraviolet emissions. A variety of UV “Black-light” ILTs were measured at a range of distances to assess potential exposures. Realistic time-weighted human exposures are shown to be well below current guidelines for human exposure to ultraviolet radiation. These UV-A exposures would be far less than the typical UV-A exposure in the outdoor environment. Proposals are made for realistic ultraviolet safety standards for ILT products. PMID:27043058

  6. 7 CFR 1230.10 - Imported.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false Imported. 1230.10 Section 1230.10 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Imported. Imported means entered, or withdrawn from a warehouse for consumption, in the customs...

  7. 7 CFR 1230.10 - Imported.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Imported. 1230.10 Section 1230.10 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Imported. Imported means entered, or withdrawn from a warehouse for consumption, in the customs...

  8. Agricultural Microbiology.

    ERIC Educational Resources Information Center

    Brill, Winston J.

    1981-01-01

    Elucidates strategies for applying microbiological techniques to traditional agricultural practices. Discusses the manipulation of microorganisms that live with plants and also the problems involved in the introduction of new genes into crop plants by recombinant DNA methods. (CS)

  9. Agricultural Geophysics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The four geophysical methods predominantly used for agricultural purposes are resistivity, electromagnetic induction, ground penetrating radar (GPR), and time domain reflectometry (TDR). Resistivity and electromagnetic induction methods are typically employed to map lateral variations of apparent so...

  10. Urban conservation agriculture

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vegetables are important sources of vitamins and nutrients for human nutrition. United States Department of Agriculture recommends filling half of the food plates with vegetables in every meal. While it is important in promoting good health, access to fresh vegetables is limited especially in urban ...

  11. Agricultural Waste.

    PubMed

    Shu, Huajie; Zhang, Panpan; Chang, Chein-Chi; Wang, Renqing; Zhang, Shuping

    2015-10-01

    The management and disposal of agricultural waste are drawn more and more attention because of the increasing yields and negative effects on the environment. However, proper treatments such as converting abundant biomass wastes into biogas through anaerobic digestion technology, can not only avoid the negative impacts, but also convert waste into available resources. This review summarizes the studies of nearly two hundred scholars from the following four aspects: the characterization, reuse, treatment, and management of agricultural waste. PMID:26420088

  12. Entomopathogenic nematodes and insect management

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Entomopathogenic nematodes (genera Heterorhabditis, Steinernema, and Neosteinernema) are used as bioinsecticides. The nematodes are ubiquitous and have been isolated in soil of every continent except Antarctica. The nematodes kill insects through a mutualism with a bacterium (Photorhabdus spp. or ...

  13. Insect symbionts in food webs

    PubMed Central

    Henry, Lee M.

    2016-01-01

    Recent research has shown that the bacterial endosymbionts of insects are abundant and diverse, and that they have numerous different effects on their hosts' biology. Here we explore how insect endosymbionts might affect the structure and dynamics of insect communities. Using the obligate and facultative symbionts of aphids as an example, we find that there are multiple ways that symbiont presence might affect food web structure. Many symbionts are now known to help their hosts escape or resist natural enemy attack, and others can allow their hosts to withstand abiotic stress or affect host plant use. In addition to the direct effect of symbionts on aphid phenotypes there may be indirect effects mediated through trophic and non-trophic community interactions. We believe that by using data from barcoding studies to identify bacterial symbionts, this extra, microbial dimension to insect food webs can be better elucidated. This article is part of the themed issue ‘From DNA barcodes to biomes’. PMID:27481779

  14. Insect symbionts in food webs.

    PubMed

    McLean, Ailsa H C; Parker, Benjamin J; Hrček, Jan; Henry, Lee M; Godfray, H Charles J

    2016-09-01

    Recent research has shown that the bacterial endosymbionts of insects are abundant and diverse, and that they have numerous different effects on their hosts' biology. Here we explore how insect endosymbionts might affect the structure and dynamics of insect communities. Using the obligate and facultative symbionts of aphids as an example, we find that there are multiple ways that symbiont presence might affect food web structure. Many symbionts are now known to help their hosts escape or resist natural enemy attack, and others can allow their hosts to withstand abiotic stress or affect host plant use. In addition to the direct effect of symbionts on aphid phenotypes there may be indirect effects mediated through trophic and non-trophic community interactions. We believe that by using data from barcoding studies to identify bacterial symbionts, this extra, microbial dimension to insect food webs can be better elucidated.This article is part of the themed issue 'From DNA barcodes to biomes'. PMID:27481779

  15. Eicosanoid actions in insect immunology

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In this chapter we review eicosanoid actions in insect immunity. Eicosanoids are oxygenated metabolites of arachidonic acid (AA) and two other C20 polyunsaturated polyunsaturated fatty acids. Groups of eicosanoids include prostaglandins, lipoxygenase products and epoxyeicosatrienoic acids. These ...

  16. Radar Observation of Insects - Mosquitoes

    NASA Technical Reports Server (NTRS)

    Frost, E.; Downing, J.

    1979-01-01

    Tests were conducted at several sites over the coastal lowlands of New Jersey and over a region of high plains and low mountains in Oklahoma. In one area, a salt marsh in New Jersey, extensive ground tests were combined with laboratory data on expected insect backscatter to arrive at an extremely convincing model of the insect origin of most Dot Angels. A great deal of insight was studied from radar on the buildup and dispersal of insect swarms, since radar can follow where other means of trapping and observation cannot. Data on large-scale behavior as a function of wind and topography are presented. Displayed techniques which show individual or small swarm motion within some larger cloud or mass, or which can show the overall motion over great distances were developed. The influence of wind and terrain on insect motion and dispersal is determined from radar data.

  17. Freshwater Biodiversity and Insect Diversification

    PubMed Central

    Dijkstra, Klaas-Douwe B.; Monaghan, Michael T.; Pauls, Steffen U.

    2016-01-01

    Inland waters cover less than one percent of Earth’s surface, but harbor more than six percent of all insect species: nearly 100,000 species from 12 orders spend one or more life stages in freshwater. Little is known about how this remarkable diversity arose, although allopatric speciation and ecological adaptation are thought to be primary mechanisms. Freshwater habitats are exceptionally susceptible to environmental change, and exhibit marked ecological gradients. The amphibiotic lifestyles of aquatic insects result in complex contributions of extinction and allopatric and non-allopatric speciation in species diversification. In contrast to the lack of evolutionary studies, the ecology and habitat preferences of aquatic insects have been intensively studied, in part because of their widespread use as bio-indicators. The combination of phylogenetics with the extensive ecological data provides a promising avenue for future research, making aquatic insects highly suitable models for the study of ecological diversification. PMID:24160433

  18. Flight of the smallest insects

    NASA Astrophysics Data System (ADS)

    Miller, Laura; Santhanakrishnan, Arvind; Hedrick, Tyson; Robinson, Alice

    2009-11-01

    A vast body of research has described the complexity of flight in insects ranging from the fruit fly, Drosophila melanogaster, to the hawk moth, Manduca sexta. Over this range of scales, flight aerodynamics as well as the relative lift and drag forces generated are surprisingly similar. The smallest flying insects (Re˜10) have received far less attention, although previous work has shown that flight kinematics and aerodynamics can be significantly different. In this presentation, we have used a three-pronged approach that consists of measurements of flight kinematics in the tiny insect Thysanoptera (thrips), measurements of flow velocities using physical models, and direct numerical simulations to compute lift and drag forces. We find that drag forces can be an order of magnitude larger than lift forces, particularly during the clap and fling motion used by all tiny insects recorded to date.

  19. Insect bites and stings (image)

    MedlinePlus

    Even though some insect bites or stings can be extremely painful they usually do not require emergency medical care. Although the stung or bitten area should be carefully observed for signs of infection or reaction to venom.

  20. Longevity of insect-killing nematodes in soil from a pecan orchard

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Entomopathogenic (insect-killing) nematodes are candidates for use as biological control agents for important pecan insect pests such as the pecan weevil, Curculio caryae. In deciding which kind of nematode (species or strain) is the best one to use, it is important to consider which one is likely t...

  1. Preface: Insect Pathology, 2nd ed

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Insect pathology is an essential component of entomology and provides a non-chemical alternative for insect pest management. There are several groups of organisms that can infect and kill insects including viruses, fungi, microsporidia, bacteria, protists, and nematodes. The dilemma in insect patho...

  2. Pollen Recovery from Insects: Light Microscopy

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Numerous insect species feed on the pollen, nectar, and other plant exudates that are associated with flowers. As a result of this feeding activity, pollen becomes attached to the insects. Analysis of the pollen attached to these insects can reveal what insects eat, their dispersal patterns in and...

  3. 46 CFR 108.215 - Insect screens.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Insect screens. 108.215 Section 108.215 Shipping COAST... Construction and Arrangement Accommodation Spaces § 108.215 Insect screens. (a) Accommodation spaces must be protected against the admission of insects. (b) Insect screens must be installed when natural ventilation...

  4. 46 CFR 108.215 - Insect screens.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Insect screens. 108.215 Section 108.215 Shipping COAST... Construction and Arrangement Accommodation Spaces § 108.215 Insect screens. (a) Accommodation spaces must be protected against the admission of insects. (b) Insect screens must be installed when natural ventilation...

  5. 46 CFR 108.215 - Insect screens.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Insect screens. 108.215 Section 108.215 Shipping COAST... Construction and Arrangement Accommodation Spaces § 108.215 Insect screens. (a) Accommodation spaces must be protected against the admission of insects. (b) Insect screens must be installed when natural ventilation...

  6. 46 CFR 108.215 - Insect screens.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Insect screens. 108.215 Section 108.215 Shipping COAST... Construction and Arrangement Accommodation Spaces § 108.215 Insect screens. (a) Accommodation spaces must be protected against the admission of insects. (b) Insect screens must be installed when natural ventilation...

  7. 46 CFR 108.215 - Insect screens.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Insect screens. 108.215 Section 108.215 Shipping COAST... Construction and Arrangement Accommodation Spaces § 108.215 Insect screens. (a) Accommodation spaces must be protected against the admission of insects. (b) Insect screens must be installed when natural ventilation...

  8. Don't Let Insects Bug You!

    ERIC Educational Resources Information Center

    Abraham, Doc; Abraham, Katy

    1977-01-01

    Are you one of those people who feel that the only good insect is a dead one? Do you suffer from entomophobia--dread fear of insects? Such attitudes, fears, and prejudices stem from insect ignorance. Authors explain what insects are good for and give students a more realistic and fascinating view of their world. (Editor/RK)

  9. Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

    PubMed Central

    Chougule, Nanasaheb P.; Li, Huarong; Liu, Sijun; Linz, Lucas B.; Narva, Kenneth E.; Meade, Thomas; Bonning, Bryony C.

    2013-01-01

    Although transgenic crops expressing Bacillus thuringiensis (Bt) toxins have been used successfully for management of lepidopteran and coleopteran pest species, the sap-sucking insects (Hemiptera) are not particularly susceptible to Bt toxins. To overcome this limitation, we demonstrate that addition of a short peptide sequence selected for binding to the gut of the targeted pest species serves to increase toxicity against said pest. Insertion of a 12-aa pea aphid gut-binding peptide by adding to or replacing amino acids in one of three loops of the Bt cytolytic toxin, Cyt2Aa, resulted in enhanced binding and toxicity against both the pea aphid, Acyrthosiphon pisum, and the green peach aphid, Myzus persicae. This strategy may allow for transgenic plant-mediated suppression of other hemipteran pests, which include some of the most important pests of global agriculture. PMID:23650347

  10. Evidence for inhibition of cholinesterases in insect and mammalian nervous systems by the insect repellent deet

    PubMed Central

    Corbel, Vincent; Stankiewicz, Maria; Pennetier, Cédric; Fournier, Didier; Stojan, Jure; Girard, Emmanuelle; Dimitrov, Mitko; Molgó, Jordi; Hougard, Jean-Marc; Lapied, Bruno

    2009-01-01

    Background N,N-Diethyl-3-methylbenzamide (deet) remains the gold standard for insect repellents. About 200 million people use it every year and over 8 billion doses have been applied over the past 50 years. Despite the widespread and increased interest in the use of deet in public health programmes, controversies remain concerning both the identification of its target sites at the olfactory system and its mechanism of toxicity in insects, mammals and humans. Here, we investigated the molecular target site for deet and the consequences of its interactions with carbamate insecticides on the cholinergic system. Results By using toxicological, biochemical and electrophysiological techniques, we show that deet is not simply a behaviour-modifying chemical but that it also inhibits cholinesterase activity, in both insect and mammalian neuronal preparations. Deet is commonly used in combination with insecticides and we show that deet has the capacity to strengthen the toxicity of carbamates, a class of insecticides known to block acetylcholinesterase. Conclusion These findings question the safety of deet, particularly in combination with other chemicals, and they highlight the importance of a multidisciplinary approach to the development of safer insect repellents for use in public health. PMID:19656357

  11. Insect repellents: historical perspectives and new developments.

    PubMed

    Katz, Tracy M; Miller, Jason H; Hebert, Adelaide A

    2008-05-01

    Arthropod bites remain a major cause of patient morbidity. These bites can cause local or systemic effects that may be infectious or inflammatory in nature. Arthropods, notably insects and arachnids, are vectors of potentially serious ailments including malaria, West Nile virus, dengue, and Lyme disease. Measures to curtail the impact of insect bites are important in the worldwide public health effort to safely protect patients and prevent the spread of disease. The history of insect repellent (IR) lends insight into some of the current scientific strategies behind newer products. Active ingredients of currently available IRs include N,N-diethyl-3-methylbenzamide (DEET), botanicals, citronella, and, the newest agent, picaridin. Currently, the Environmental Protection Agency's registered IR ingredients approved for application to the skin include DEET, picaridin, MGK-326, MGK-264, IR3535, oil of citronella, and oil of lemon eucalyptus. DEET has reigned as the most efficacious and broadly used IR for the last 6 decades, with a strong safety record and excellent protection against ticks, mosquitoes, and other arthropods. Newer agents, like picaridin and natural products such as oil of lemon eucalyptus are becoming increasingly popular because of their low toxicity, comparable efficacy, and customer approval. Various characteristics and individual product advantages may lead physicians to recommend one agent over another. PMID:18272250

  12. 7 CFR 201.38 - Importations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Importations. 201.38 Section 201.38 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED...

  13. 7 CFR 1210.502 - Importer members.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false Importer members. 1210.502 Section 1210.502 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE WATERMELON RESEARCH...

  14. 7 CFR 1210.502 - Importer members.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 10 2014-01-01 2014-01-01 false Importer members. 1210.502 Section 1210.502 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE WATERMELON RESEARCH...

  15. 7 CFR 1210.502 - Importer members.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false Importer members. 1210.502 Section 1210.502 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE WATERMELON RESEARCH...

  16. 7 CFR 926.15 - Importer.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Importer. 926.15 Section 926.15 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE DATA COLLECTION, REPORTING...

  17. Agricultural Awareness Activities and Their Integration into the Curriculum as Perceived by Elementary Teachers.

    ERIC Educational Resources Information Center

    Knobloch, Neil A.; Martin, Robert A.

    2000-01-01

    Responses from 281 of 689 elementary teachers indicated they had positive perceptions of the agriculture industry and integration of agriculture into the curriculum. Over 80% used agriculture activities, especially the study of animals, plants, food, nutrition, environment, wildlife, and insects. (Contains 38 references.) (SK)

  18. Usefulness of the insect food in the long-term space stay

    NASA Astrophysics Data System (ADS)

    Katayama, Naomi; Yamashita, Masamichi

    2016-07-01

    The meal is important in life in the space. The importance of space foods is not only health maintenance. The space foods are one of the Life-support system for a space trip. Time for meal is time of the relaxation of home life of the astronaut. However, the breeding of the large animal is still impossible in the spaceship now narrowly. If it is fish and an insect, the breeding in the spaceship is possible. We recognize an insect as ingredients on the earth. As for the insect, possibility to save a food shortage of the earth is expected in future. We suggested the space foods using the insect for 12 years. The cultivation of the insect is pushed forward now in Europe. We suggest a menu to have you know the space foods which took in an insect more. The insect which we used for this menu is silkworm-pupa, a grasshopper, a larva of a wasp and apple snail. The Japanese foods were registered with world's cultural heritage. Therefore we used an insect to make our Japanese foods. Space foods must be universal food. This is because the astronauts are recruited from the whole world. Space foods that a world astronaut eats and thinks to be delicious are necessary. We want to take in an insect in world cooking in future. The insect food includes essential amino acids and essential fatty acid. The insect is superior nutritionally. We will think that insect food is necessary more and more on both the space and the earth in future. The insect is precious ingredients relieving a food shortage for the human.

  19. Social Insects: A Model System for Network Dynamics

    NASA Astrophysics Data System (ADS)

    Charbonneau, Daniel; Blonder, Benjamin; Dornhaus, Anna

    Social insect colonies (ants, bees, wasps, and termites) show sophisticated collective problem-solving in the face of variable constraints. Individuals exchange information and materials such as food. The resulting network structure and dynamics can inform us about the mechanisms by which the insects achieve particular collective behaviors and these can be transposed to man-made and social networks. We discuss how network analysis can answer important questions about social insects, such as how effective task allocation or information flow is realized. We put forward the idea that network analysis methods are under-utilized in social insect research, and that they can provide novel ways to view the complexity of collective behavior, particularly if network dynamics are taken into account. To illustrate this, we present an example of network tasks performed by ant workers, linked by instances of workers switching from one task to another. We show how temporal network analysis can propose and test new hypotheses on mechanisms of task allocation, and how adding temporal elements to static networks can drastically change results. We discuss the benefits of using social insects as models for complex systems in general. There are multiple opportunities emergent technologies and analysis methods in facilitating research on social insect network. The potential for interdisciplinary work could significantly advance diverse fields such as behavioral ecology, computer sciences, and engineering.

  20. Synergistic interactions of ecosystem services: florivorous pest control boosts crop yield increase through insect pollination.

    PubMed

    Sutter, Louis; Albrecht, Matthias

    2016-02-10

    Insect pollination and pest control are pivotal functions sustaining global food production. However, they have mostly been studied in isolation and how they interactively shape crop yield remains largely unexplored. Using controlled field experiments, we found strong synergistic effects of insect pollination and simulated pest control on yield quantity and quality. Their joint effect increased yield by 23%, with synergistic effects contributing 10%, while their single contributions were 7% and 6%, respectively. The potential economic benefit for a farmer from the synergistic effects (12%) was 1.8 times greater than their individual contributions (7% each). We show that the principal underlying mechanism was a pronounced pest-induced reduction in flower lifetime, resulting in a strong reduction in the number of pollinator visits a flower receives during its lifetime. Our findings highlight the importance of non-additive interactions among ecosystem services (ES) when valuating, mapping or predicting them and reveal fundamental implications for ecosystem management and policy aimed at maximizing ES for sustainable agriculture. PMID:26865304

  1. Hermes, a functional non-Drosophilid insect gene vector from Musca domestica.

    PubMed

    O'Brochta, D A; Warren, W D; Saville, K J; Atkinson, P W

    1996-03-01

    Hermes is a short inverted repeat-type transposable element from the house fly, Musca domestica. Using an extra-chromosomal transpositional recombination assay, we show that Hermes elements can accurately transpose in M. domestica embryos. To test the ability of Hermes to function in species distantly related to M. domestica we used a nonautonomous Hermes element containing the Drosophila melanogaster while (w+) gene and created D. melanogaster germline transformants. Transgenic G1 insects were recovered from 34.6% of the fertile G0 adults developing from microinjected w- embryos. This transformation rate is comparable with that observed using P or hobo vectors in D. melanogaster, however, many instances of multiple-element insertions and large clusters were observed. Genetic mapping, Southern blotting, polytene chromosome in situ hybridization and DNA sequence analyses confirmed that Hermes elements were chromosomally integrated in transgenic insects. Our data demonstrate that Hermes elements transpose at high rates in D. melanogaster and may be an effective gene vector and gene-tagging agent in this species and distantly related species of medical and agricultural importance. PMID:8849896

  2. Hermes, a Functional Non-Drosophilid Insect Gene Vector from Musca Domestica

    PubMed Central

    O'Brochta, D. A.; Warren, W. D.; Saville, K. J.; Atkinson, P. W.

    1996-01-01

    Hermes is a short inverted repeat-type transposable element from the house fly, Musca domestica. Using an extra-chromosomal transpositional recombination assay, we show that Hermes elements can accurately transpose in M. domestica embryos. To test the ability of Hermes to function in species distantly related to M. domestica we used a nonautonomous Hermes element containing the Drosophila melanogaster white (w(+)) gene and created D. melanogaster germline transformants. Transgenic G(1) insects were recovered from 34.6% of the fertile G(0) adults developing from microinjected w(-) embryos. This transformation rate is comparable with that observed using P or hobo vectors in D. melanogaster, however, many instances of multiple-element insertions and large clusters were observed. Genetic mapping, Southern blotting, polytene chromosome in situ hybridization and DNA sequence analyses confirmed that Hermes elements were chromosomally integrated in transgenic insects. Our data demonstrate that Hermes elements transpose at high rates in D. melanogaster and may be an effective gene vector and gene-tagging agent in this species and distantly related species of medical and agricultural importance. PMID:8849896

  3. Hermes, a functional non-drosophilid insect gene vector from Musca domestica

    SciTech Connect

    O`Brochta, D.A.; Warren, W.D.; Saville, K.J.

    1996-03-01

    Hermes is a short inverted repeat-type transposable element from the house fly, Musca domestica. Using an extra-chromosomal transpositional recombination assay, we show that Hermes elements can accurately transpose in M. domestica embryos. To test the ability of Hermes to function in species distantly related to M. domestica we used a nonautonomous Hermes element containing the Drosophila melanogaster white (w{sup +}) gene and created D. melanogaster germline transformants. Transgenic G{sub 1} insects were recovered from 34.6% of the fertile G{sub 0} adults developing from microinjected w{sup -} embryos. This transformation rate is comparable with that observed using P or hobo vectors in D. melanogaster; however, many instances of multiple-element insertions and large clusters were observed. Genetic mapping, Southern blotting, polytene chromosome in situ hybridization and DNA sequence analyses confirmed that Hermes elements were chromosomally integrated in transgenic insects. Our data demonstrate that Hermes elements transpose at high rates in D. melanogaster and may be an effective gene vector and gene-tagging agent in this species and distantly related species of medical and agricultural importance. 46 refs., 5 figs., 1 tab.

  4. Biodiversity of Aquatic Insects of Zayandeh Roud River and Its Branches, Isfahan Province, Iran

    PubMed Central

    Shayeghi, Mansoreh; Vatandoost, Hassan; Gorouhi, Abdollah; Sanei-Dehkordi, Ali Reza; Salim-Abadi, Yaser; Karami, Mohsen; Jalil-Navaz, Mohammad Reza; Akhavan, Amir Ahmad; Shiekh, Zahra; Vatandoost, Sajad; Arandian, Mohammad Hossein

    2014-01-01

    Background: Aquatic insects are the major groups of arthropods that spend some parts of their life cycle in the water. These insects play an important role for transmission of some human and animal diseases. There is few information about the aquatic insects fauna of Iran. Methods: To study the aquatic insects fauna, adult, nymphal and larval collections were carried out from different habitats using the standard technique in Zayandeh Roud River, Isfahan Province,central Iran, during summer 2011. Results: In total, 741 speimens of aquatic insects were collected and morphologically identified. They include 7 families and 12 genera representing 2 Orders. The order of Diptera (92.31%) and Coleoptera (7.69%). The families Culicidae, Syrphidae and Chironomidae from Diptera order, Gyrinidae, Dytiscidae, Haliplidae, Hydrophilidae from Coleoptera order were identified. Conclusion: Some aquatic insects play an important role for transmission of human and animal diseases. These insects also are important for biological control. Therefore ecological study on aquatic insects can provide information about ecology of insects in an area for any decision making. PMID:26114133

  5. Neonicotinoid clothianidin adversely affects insect immunity and promotes replication of a viral pathogen in honey bees.

    PubMed

    Di Prisco, Gennaro; Cavaliere, Valeria; Annoscia, Desiderato; Varricchio, Paola; Caprio, Emilio; Nazzi, Francesco; Gargiulo, Giuseppe; Pennacchio, Francesco

    2013-11-12

    Large-scale losses of honey bee colonies represent a poorly understood problem of global importance. Both biotic and abiotic factors are involved in this phenomenon that is often associated with high loads of parasites and pathogens. A stronger impact of pathogens in honey bees exposed to neonicotinoid insecticides has been reported, but the causal link between insecticide exposure and the possible immune alteration of honey bees remains elusive. Here, we demonstrate that the neonicotinoid insecticide clothianidin negatively modulates NF-κB immune signaling in insects and adversely affects honey bee antiviral defenses controlled by this transcription factor. We have identified in insects a negative modulator of NF-κB activation, which is a leucine-rich repeat protein. Exposure to clothianidin, by enhancing the transcription of the gene encoding this inhibitor, reduces immune defenses and promotes the replication of the deformed wing virus in honey bees bearing covert infections. This honey bee immunosuppression is similarly induced by a different neonicotinoid, imidacloprid, but not by the organophosphate chlorpyriphos, which does not affect NF-κB signaling. The occurrence at sublethal doses of this insecticide-induced viral proliferation suggests that the studied neonicotinoids might have a negative effect at the field level. Our experiments uncover a further level of regulation of the immune response in insects and set the stage for studies on neural modulation of immunity in animals. Furthermore, this study has implications for the conservation of bees, as it will contribute to the definition of more appropriate guidelines for testing chronic or sublethal effects of pesticides used in agriculture. PMID:24145453

  6. O antigen modulates insect vector acquisition of the bacterial plant pathogen Xylella fastidiosa.

    PubMed

    Rapicavoli, Jeannette N; Kinsinger, Nichola; Perring, Thomas M; Backus, Elaine A; Shugart, Holly J; Walker, Sharon; Roper, M Caroline

    2015-12-01

    Hemipteran insect vectors transmit the majority of plant pathogens. Acquisition of pathogenic bacteria by these piercing/sucking insects requires intimate associations between the bacterial cells and insect surfaces. Lipopolysaccharide (LPS) is the predominant macromolecule displayed on the cell surface of Gram-negative bacteria and thus mediates bacterial interactions with the environment and potential hosts. We hypothesized that bacterial cell surface properties mediated by LPS would be important in modulating vector-pathogen interactions required for acquisition of the bacterial plant pathogen Xylella fastidiosa, the causative agent of Pierce's disease of grapevines. Utilizing a mutant that produces truncated O antigen (the terminal portion of the LPS molecule), we present results that link this LPS structural alteration to a significant decrease in the attachment of X. fastidiosa to blue-green sharpshooter foreguts. Scanning electron microscopy confirmed that this defect in initial attachment compromised subsequent biofilm formation within vector foreguts, thus impairing pathogen acquisition. We also establish a relationship between O antigen truncation and significant changes in the physiochemical properties of the cell, which in turn affect the dynamics of X. fastidiosa adhesion to the vector foregut. Lastly, we couple measurements of the physiochemical properties of the cell with hydrodynamic fluid shear rates to produce a Comsol model that predicts primary areas of bacterial colonization within blue-green sharpshooter foreguts, and we present experimental data that support the model. These results demonstrate that, in addition to reported protein adhesin-ligand interactions, O antigen is crucial for vector-pathogen interactions, specifically in the acquisition of this destructive agricultural pathogen. PMID:26386068

  7. A novel approach to evaluation of pest insect abundance in the presence of noise.

    PubMed

    Embleton, Nina; Petrovskaya, Natalia

    2014-03-01

    Evaluation of pest abundance is an important task of integrated pest management. It has recently been shown that evaluation of pest population size from discrete sampling data can be done by using the ideas of numerical integration. Numerical integration of the pest population density function is a computational technique that readily gives us an estimate of the pest population size, where the accuracy of the estimate depends on the number of traps installed in the agricultural field to collect the data. However, in a standard mathematical problem of numerical integration, it is assumed that the data are precise, so that the random error is zero when the data are collected. This assumption does not hold in ecological applications. An inherent random error is often present in field measurements, and therefore it may strongly affect the accuracy of evaluation. In our paper, we offer a novel approach to evaluate the pest insect population size under the assumption that the data about the pest population include a random error. The evaluation is not based on statistical methods but is done using a spatially discrete method of numerical integration where the data obtained by trapping as in pest insect monitoring are converted to values of the population density. It will be discussed in the paper how the accuracy of evaluation differs from the case where the same evaluation method is employed to handle precise data. We also consider how the accuracy of the pest insect abundance evaluation can be affected by noise when the data available from trapping are sparse. In particular, we show that, contrary to intuitive expectations, noise does not have any considerable impact on the accuracy of evaluation when the number of traps is small as is conventional in ecological applications. PMID:24619808

  8. O Antigen Modulates Insect Vector Acquisition of the Bacterial Plant Pathogen Xylella fastidiosa

    PubMed Central

    Rapicavoli, Jeannette N.; Kinsinger, Nichola; Perring, Thomas M.; Backus, Elaine A.; Shugart, Holly J.; Walker, Sharon

    2015-01-01

    Hemipteran insect vectors transmit the majority of plant pathogens. Acquisition of pathogenic bacteria by these piercing/sucking insects requires intimate associations between the bacterial cells and insect surfaces. Lipopolysaccharide (LPS) is the predominant macromolecule displayed on the cell surface of Gram-negative bacteria and thus mediates bacterial interactions with the environment and potential hosts. We hypothesized that bacterial cell surface properties mediated by LPS would be important in modulating vector-pathogen interactions required for acquisition of the bacterial plant pathogen Xylella fastidiosa, the causative agent of Pierce's disease of grapevines. Utilizing a mutant that produces truncated O antigen (the terminal portion of the LPS molecule), we present results that link this LPS structural alteration to a significant decrease in the attachment of X. fastidiosa to blue-green sharpshooter foreguts. Scanning electron microscopy confirmed that this defect in initial attachment compromised subsequent biofilm formation within vector foreguts, thus impairing pathogen acquisition. We also establish a relationship between O antigen truncation and significant changes in the physiochemical properties of the cell, which in turn affect the dynamics of X. fastidiosa adhesion to the vector foregut. Lastly, we couple measurements of the physiochemical properties of the cell with hydrodynamic fluid shear rates to produce a Comsol model that predicts primary areas of bacterial colonization within blue-green sharpshooter foreguts, and we present experimental data that support the model. These results demonstrate that, in addition to reported protein adhesin-ligand interactions, O antigen is crucial for vector-pathogen interactions, specifically in the acquisition of this destructive agricultural pathogen. PMID:26386068

  9. Emerging Agricultural Biotechnologies for Sustainable Agriculture and Food Security.

    PubMed

    Anderson, Jennifer A; Gipmans, Martijn; Hurst, Susan; Layton, Raymond; Nehra, Narender; Pickett, John; Shah, Dilip M; Souza, Thiago Lívio P O; Tripathi, Leena

    2016-01-20

    As global populations continue to increase, agricultural productivity will be challenged to keep pace without overtaxing important environmental resources. A dynamic and integrated approach will be required to solve global food insecurity and position agriculture on a trajectory toward sustainability. Genetically modified (GM) crops enhanced through modern biotechnology represent an important set of tools that can promote sustainable agriculture and improve food security. Several emerging biotechnology approaches were discussed in a recent symposium organized at the 13th IUPAC International Congress of Pesticide Chemistry meeting in San Francisco, CA, USA. This paper summarizes the innovative research and several of the new and emerging technologies within the field of agricultural biotechnology that were presented during the symposium. This discussion highlights how agricultural biotechnology fits within the context of sustainable agriculture and improved food security and can be used in support of further development and adoption of beneficial GM crops. PMID:26785813

  10. Advances and perspectives in the application of CRISPR/Cas9 in insects.

    PubMed

    Chen, Lei; Wang, Gui; Zhu, Ya-Nan; Xiang, Hui; Wang, Wen

    2016-07-18

    Insects compose more than half of all living organisms on earth, playing essential roles in global ecosystems and forming complex relationships with humans. Insect research has significant biological and practical importance. However, the application of genetic manipulation technology has long been restricted to several model insects only, such as gene knockout in Drosophila, which has severely restrained the development of insect biology research. Recently, with the increase in the release of insect genome data and the introduction of the CRISPR/Cas9 system for efficient genetic modification, it has been possible to conduct meaningful functional studies in a broad array of insect species. Here, we summarize the advances in CRISPR/Cas9 in different insect species, discuss methods for its promotion, and consider its application in future insect studies. This review provides detailed information about the application of the CRISPR/Cas9 system in insect research and presents possible ways to improve its use in functional studies and insect pest control. PMID:27469253

  11. Advances and perspectives in the application of CRISPR/Cas9 in insects

    PubMed Central

    CHEN, Lei; WANG, Gui; ZHU, Ya-Nan; XIANG, Hui; WANG, Wen

    2016-01-01

    Insects compose more than half of all living organisms on earth, playing essential roles in global ecosystems and forming complex relationships with humans. Insect research has significant biological and practical importance. However, the application of genetic manipulation technology has long been restricted to several model insects only, such as gene knockout in Drosophila, which has severely restrained the development of insect biology research. Recently, with the increase in the release of insect genome data and the introduction of the CRISPR/Cas9 system for efficient genetic modification, it has been possible to conduct meaningful functional studies in a broad array of insect species. Here, we summarize the advances in CRISPR/Cas9 in different insect species, discuss methods for its promotion, and consider its application in future insect studies. This review provides detailed information about the application of the CRISPR/Cas9 system in insect research and presents possible ways to improve its use in functional studies and insect pest control.

  12. Macronutrient contributions of insects to the diets of hunter-gatherers: a geometric analysis.

    PubMed

    Raubenheimer, David; Rothman, Jessica M; Pontzer, Herman; Simpson, Stephen J

    2014-06-01

    We present a geometric model for examining the macronutrient contributions of insects in the diets of pre-agricultural humans, and relate the findings to some contemporary societies that regularly eat insects. The model integrates published data on the macronutrient composition of insects and other foods in the diets of humans, recommended human macronutrient intakes, and estimated macronutrient intakes to examine the assumption that insects provided to pre-agricultural humans an invertebrate equivalent of vertebrate-derived meats, serving primarily as a source of protein. Our analysis suggests that insects vary more widely in their macronutrient content than is likely to be the case for most wild vertebrate meats, spanning a broad range of protein, fat and carbohydrate concentrations. Potentially, therefore, in terms of their proportional macronutrient composition, insects could serve as equivalents not only of wild meat, but of a range of other foods including some shellfish, nuts, pulses, vegetables and even fruits. Furthermore, humans might systematically manipulate the composition of edible insects to meet specific needs through pre-ingestive processing, such as cooking and selective removal of body parts. We present data suggesting that in modern societies for which protein is the more limiting macronutrient, pre-ingestive processing of edible insects might serve to concentrate protein. It is likely, however, that the dietary significance of insects was different for Paleolithic hunter-gatherers who were more limited in non-protein energy. Our conclusions are constrained by available data, but highlight the need for further studies, and suggest that our model provides an integrative framework for conceiving these studies. PMID:24630913

  13. Discontinuous gas exchange in insects.

    PubMed

    Quinlan, Michael C; Gibbs, Allen G

    2006-11-01

    Insect respiratory physiology has been studied for many years, and interest in this area of insect biology has become revitalized recently for a number of reasons. Technical advances have greatly improved the precision, accuracy and ease with which gas exchange can be measured in insects. This has made it possible to go beyond classic models such as lepidopteran pupae and examine a far greater diversity of species. One striking result of recent work is the realization that insect gas exchange patterns are much more diverse than formerly recognized. Current work has also benefited from the inclusion of comparative methods that rigorously incorporate phylogenetic, ecological and life history information. We discuss these advances in the context of the classic respiratory pattern of insects, discontinuous gas exchange. This mode of gas exchange was exhaustively described in moth pupae in the 1950s and 1960s. Early workers concluded that discontinuous gas exchange was an adaptation to reduce respiratory water loss. This idea is no longer universally accepted, and several competing hypotheses have been proposed. We discuss the genesis of these alternative hypotheses, and we identify some of the predictions that might be used to test them. We are pleased to report that what was once a mature discipline, in which the broad parameters and adaptive significance of discontinuous gas exchange were thought to be well understood, is now a thriving and vigorous field of research. PMID:16870512

  14. Agricultural Biodiversity.

    ERIC Educational Resources Information Center

    Postance, Jim

    1998-01-01

    The extinction of farm animals and crops is rarely brought up during discussions of endangered species and biodiversity; however, the loss of diversity in crops and livestock threatens the sustainability of agriculture. Presents three activities: (1) "The Colors of Diversity"; (2) "Biodiversity among Animals"; and (3) "Heirloom Plants." Discusses…

  15. AGRICULTURAL EXTENSION.

    ERIC Educational Resources Information Center

    FARQUHAR, R.N.

    AUSTRALIAN AGRICULTURAL EXTENSION HAS LONG EMPHASIZED TECHNICAL ADVISORY SERVICE AT THE EXPENSE OF THE SOCIOECONOMIC ASPECTS OF FARM PRODUCTION AND FARM LIFE. ONLY IN TASMANIA HAS FARM MANAGEMENT BEEN STRESSED. DEMANDS FOR THE WHOLE-FARM APPROACH HAVE PRODUCED A TREND TOWARD GENERALISM FOR DISTRICT OFFICERS IN MOST STATES. THE FEDERAL GOVERNMENT,…

  16. AGRICULTURAL EDUCATION.

    ERIC Educational Resources Information Center

    DEALTON, ERNEST L.

    TODAY'S SUCCESSFUL FARMER MUST POSSESS THE SKILLS OF A BUSINESSMAN, SCIENTIST, AND MECHANIC TO SURVIVE COMPETITION IN AGRICULTURE, THE LARGEST INDUSTRY IN THE UNITED STATES. THIS COMPETITION HAS CAUSED AN INCREASE IN THE SIZE OF FARMS AND RANCHES IN AN ATTEMPT TO CURTAIL OPERATIONAL EXPENSES AND TO INCREASE PRODUCTION. WITH THE SCIENTIFIC…

  17. Aphis glycines & Halyomorpha halys: Asian agricultural pests recently established in the North America

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Despite regulatory inspections and quarantine, the invasion of insects in new geographic regions continues to cause problems. Several Asian insects are recent examples of invasive agricultural pests in North America. Soybean aphid, Aphis glycines, widespread throughout China, Korea, and Japan, was...

  18. Phenoptosis in arthropods and immortality of social insects.

    PubMed

    Kartsev, V M

    2014-10-01

    In general, there are no drastic differences in phenoptosis patterns in plant and animal organisms. However, there are some specific features characteristic for insects and other arthropods: 1) their development includes metamorphosis with different biochemical laws at consecutive developmental stages; 2) arthropods can reduce or stop development and aging when in a state of diapause or temporal cold immobility; 3) their life cycle often correlates with seasonal changes of surroundings; 4) polymorphism is widespread - conspecifics differ by their lifespans and phenoptosis features; 5) lifespan-related sexual dimorphism is common; 6) significant situational plasticity of life cycle organization is an important feature; for example, the German wasp (Paravespula germanica) is obligatorily univoltine in the temperate zone, while in tropical regions its lifespan increases and leads to repeated reproduction; 7) life cycles of closely related species may differ significantly, for example, in contrast to German wasp, some tropical hornets (Vespa) have only one reproduction period. Surprisingly, many insect species have been shown to be subjected to gradual aging and phenoptosis, like the highest mammals. However, queens of social insects and some long-lived arachnids can apparently be considered non-aging organisms. In some species, lifespan is limited to one season, while others live much longer or shorter. Cases of one-time reproduction are rather rare. Aphagia is common in insects (over 10,000 species). Cannibalism is an important mortality factor in insects as well as in spiders. In social insects, which exist only in colonies (families), the lifetime of a colony can be virtually unlimited. However, in case of some species the developmental cycle and death of a colony after its completion are predetermined. Most likely, natural selection in insects does not lengthen individual lifespan, but favors increase in reproduction efficiency based on fast succession of

  19. The role of airfoil geometry in minimizing the effect of insect contamination of laminar flow sections

    NASA Technical Reports Server (NTRS)

    Maresh, J. L.; Bragg, M. B.

    1984-01-01

    A method has been developed to predict the contamination of an airfoil by insects and the resultant performance penalty. Insect aerodynamics have been modeled and the impingement of insects on an airfoil are solved by calculating their trajectories. Upon impact, insect rupture and the resulting height of the debris is determined based on experimental data. A boundary layer analysis is performed to determine which insects cause boundary layer transition and the resultant drag penalty. A contaminated airfoil figure of merit is presented to be used to compare airfoil susceptibility. Results show that the insect contamination effects depend on accretion conditions, airfoil angle of attack and Reynolds number. The importance of the stagnation region to designing airfoils for minimum drag penalties is discussed.

  20. Line following terrestrial insect biobots.

    PubMed

    Latif, Tahmid; Bozkurt, Alper

    2012-01-01

    The present day technology falls short in offering centimeter scale mobile robots that can function effectively under unknown and dynamic environmental conditions. Insects, on the other hand, exhibit an unmatched ability to navigate through a wide variety of environments and overcome perturbations by successfully maintaining control and stability. In this study, we use neural stimulation systems to wirelessly navigate cockroaches to follow lines to enable terrestrial insect biobots. We also propose a system-on-chip based ZigBee enabled wireless neurostimulation backpack system with on-board tissue-electrode bioelectrical coupling verification. Such a capability ensures an electrochemically safe stimulation and avoids irreversible damage to the interface which is often misinterpreted as habituation of the insect to the applied stimulation. PMID:23366056

  1. Rice Reoviruses in Insect Vectors.

    PubMed

    Wei, Taiyun; Li, Yi

    2016-08-01

    Rice reoviruses, transmitted by leafhopper or planthopper vectors in a persistent propagative manner, seriously threaten the stability of rice production in Asia. Understanding the mechanisms that enable viral transmission by insect vectors is a key to controlling these viral diseases. This review describes current understanding of replication cycles of rice reoviruses in vector cell lines, transmission barriers, and molecular determinants of vector competence and persistent infection. Despite recent breakthroughs, such as the discoveries of actin-based tubule motility exploited by viruses to overcome transmission barriers and mutually beneficial relationships between viruses and bacterial symbionts, there are still many gaps in our knowledge of transmission mechanisms. Advances in genome sequencing, reverse genetics systems, and molecular technologies will help to address these problems. Investigating the multiple interaction systems among the virus, insect vector, insect symbiont, and plant during natural infection in the field is a central topic for future research on rice reoviruses. PMID:27296147

  2. Flower constancy in insect pollinators

    PubMed Central

    Ratnieks, Francis L.W.

    2011-01-01

    As first noted by Aristotle in honeybee workers, many insect pollinators show a preference to visit flowers of just one species during a foraging trip. This “flower constancy” probably benefits plants, because pollen is more likely to be deposited on conspecific stigmas. But it is less clear why insects should ignore rewarding alternative flowers. Many researchers have argued that flower constancy is caused by constraints imposed by insect nervous systems rather than because flower constancy is itself an efficient foraging method. We argue that this view is unsatisfactory because it both fails to explain why foragers flexibly adjust the degree of flower constancy and does not explain why foragers of closely related species show different degrees of constancy. While limitations of the nervous system exist and are likely to influence flower constancy to some degree, the observed behavioural flexibility suggests that flower constancy is a successful foraging strategy given the insect’s own information about different foraging options. PMID:22446521

  3. Factors affecting the sticking of insects on modified aircraft wings

    NASA Technical Reports Server (NTRS)

    Yi, O.; Chitsaz-Z, M. R.; Eiss, N. S.; Wightman, J. P.

    1987-01-01

    Past studies have shown that the surface energy of a polymer coating has an important effect on the sticking of insects to the surface. However, mechanical properties of polymer coatings such as elasticity may also be important. A further study is suggested using polymer coatings of known surface energy and modulus so that a better understanding of the mechanism of the sticking of insects to surfaces can be achieved. As the first step for the study, surface analysis and road tests were performed using elastomers having different energies and different moduli. The number of insects sticking to each elastomer was counted and compared from sample to sample and with a control (aluminum). An average height moment was also calculated and comparisons made between samples.

  4. Shelter-Building Insects and Their Role as Ecosystem Engineers.

    PubMed

    Cornelissen, T; Cintra, F; Santos, J C

    2016-02-01

    Amelioration of harsh conditions, manipulation of host plant quality, and protection from natural enemies have all been suggested as potential forces in the evolution and maintenance of concealed feeding in insects. The construction of shelters--either in the form of mines, galls, and leaf rolls--are expected to increase larval survivorship and might influence other organisms of the community through non-trophic direct and indirect effects when shelters are co-occupied or occupied after abandonment, placing leaf and stem shelter-builders within the context of ecosystem engineering. In this review, we evaluate the potential of shelter built by insects to reduce pressure exerted by natural enemies, increase tissue quality, and provide shelter against abiotic conditions experienced during insect development. Through a quantitative analysis, we also examined the effects of insect shelters on patterns of richness and abundance of local communities, reviewing the data published in the last 15 years. We demonstrate strong effects of shelters on several arthropods, with increased richness and abundance when shelters are present in the host plants. These results reinforce the importance of the physical structures created by insects that although subtle, might have important roles in facilitative interactions. PMID:26631227

  5. Multi-factor climate change effects on insect herbivore performance

    PubMed Central

    Scherber, Christoph; Gladbach, David J; Stevnbak, Karen; Karsten, Rune Juelsborg; Schmidt, Inger Kappel; Michelsen, Anders; Albert, Kristian Rost; Larsen, Klaus Steenberg; Mikkelsen, Teis Nørgaard; Beier, Claus; Christensen, Søren

    2013-01-01

    The impact of climate change on herbivorous insects can have far-reaching consequences for ecosystem processes. However, experiments investigating the combined effects of multiple climate change drivers on herbivorous insects are scarce. We independently manipulated three climate change drivers (CO2, warming, drought) in a Danish heathland ecosystem. The experiment was established in 2005 as a full factorial split-plot with 6 blocks × 2 levels of CO2 × 2 levels of warming × 2 levels of drought = 48 plots. In 2008, we exposed 432 larvae (n = 9 per plot) of the heather beetle (Lochmaea suturalis Thomson), an important herbivore on heather, to ambient versus elevated drought, temperature, and CO2 (plus all combinations) for 5 weeks. Larval weight and survival were highest under ambient conditions and decreased significantly with the number of climate change drivers. Weight was lowest under the drought treatment, and there was a three-way interaction between time, CO2, and drought. Survival was lowest when drought, warming, and elevated CO2 were combined. Effects of climate change drivers depended on other co-acting factors and were mediated by changes in plant secondary compounds, nitrogen, and water content. Overall, drought was the most important factor for this insect herbivore. Our study shows that weight and survival of insect herbivores may decline under future climate. The complexity of insect herbivore responses increases with the number of combined climate change drivers. PMID:23789058

  6. Stinging Insect Allergy

    MedlinePlus

    ... it is important to identify them. Yellow jackets' nests are made of a paper-maché material and ... hollow trees or cavities of buildings. Paper wasps' nests are usually made of a paper-like material ...

  7. Transcriptome analyses of insect cells to facilitate baculovirus-insect expression.

    PubMed

    Yu, Kai; Yu, Yang; Tang, Xiaoyan; Chen, Huimin; Xiao, Junyu; Su, Xiao-Dong

    2016-05-01

    The High Five cell line (BTI-TN-5B1-4) isolated from the cabbage looper, Trichoplusia ni is an insect cell line widely used for baculovirus-mediated recombinant protein expression. Despite its widespread application in industry and academic laboratories, the genomic background of this cell line remains unclear. Here we sequenced the transcriptome of High Five cells and assembled 25,234 transcripts. Codon usage analysis showed that High Five cells have a robust codon usage capacity and therefore suit for expressing proteins of both eukaryotic- and prokaryotic-origin. Genes involved in glycosylation were profiled in our study, providing guidance for engineering glycosylated proteins in the insect cells. We also predicted signal peptides for transcripts with high expression abundance in both High Five and Sf21 cell lines, and these results have important implications for optimizing the expression level of some secretory and membrane proteins. PMID:27017378

  8. Insect growth regulators and insect control: a critical appraisal.

    PubMed Central

    Siddall, J B

    1976-01-01

    Insect growth regulators (IGRs) of the juvenile hormone type alter physiological processes essential to insect development and appear to act specifically on insects. Three natural juvenile hormones have been found in insects but not in other organisms. Future use of antagonists or inhibitors of hormone synthesis may be technically possible as an advantageous extension of pest control by IGRs. A documented survey of the properties, metabolism, toxicology, and uses of the most commercially advanced chemical, methoprene, shows it to be environmentally acceptable and toxicologically innocuous. Derivation of its current use patterns is discussed and limitations on these are noted. Residue levels and their measurement in the ppb region have allowed exemption from the requirement of tolerances in the EPA registered use of methoprene for mosquito control. Tolerances for foods accompany its fully approved use for control of manure breeding flies through a cattle feed supplement. The human health effects of using this chemical appear to be purely beneficial, but further advances through new IGR chemicals appear unlikely without major changes in regulatory and legislative policy. PMID:976222

  9. Insect Screening Results: Assessment of Corn Hybrids for Insect Resistance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    During the growing season of 2006, the relatively dry environmental conditions in Tifton, Georgia were favorable for the rapid buildup of corn earworms, providing the potential for considerable damage to the corn crop. Six ear-feeding insects recorded in the order of infestation severity were: the...

  10. A Field Study in Benin to Investigate the Role of Mosquitoes and Other Flying Insects in the Ecology of Mycobacterium ulcerans

    PubMed Central

    Zogo, Barnabas; Djenontin, Armel; Carolan, Kevin; Babonneau, Jeremy; Guegan, Jean-François; Eyangoh, Sara; Marion, Estelle

    2015-01-01

    Background Buruli ulcer, the third mycobacterial disease after tuberculosis and leprosy, is caused by the environmental mycobacterium M. ulcerans. There is at present no clear understanding of the exact mode(s) of transmission of M. ulcerans. Populations affected by Buruli ulcer are those living close to humid and swampy zones. The disease is associated with the creation or the extension of swampy areas, such as construction of dams or lakes for the development of agriculture. Currently, it is supposed that insects (water bugs and mosquitoes) are host and vector of M. ulcerans. The role of water bugs was clearly demonstrated by several experimental and environmental studies. However, no definitive conclusion can yet be drawn concerning the precise importance of this route of transmission. Concerning the mosquitoes, DNA was detected only in mosquitoes collected in Australia, and their role as host/vector was never studied by experimental approaches. Surprisingly, no specific study was conducted in Africa. In this context, the objective of this study was to investigate the role of mosquitoes (larvae and adults) and other flying insects in ecology of M. ulcerans. This study was conducted in a highly endemic area of Benin. Methodology/Principal Findings Mosquitoes (adults and larvae) were collected over one year, in Buruli ulcer endemic in Benin. In parallel, to monitor the presence of M. ulcerans in environment, aquatic insects were sampled. QPCR was used to detected M. ulcerans DNA. DNA of M. ulcerans was detected in around 8.7% of aquatic insects but never in mosquitoes (larvae or adults) or in other flying insects. Conclusion/Significance This study suggested that the mosquitoes don't play a pivotal role in the ecology and transmission of M. ulcerans in the studied endemic areas. However, the role of mosquitoes cannot be excluded and, we can reasonably suppose that several routes of transmission of M. ulcerans are possible through the world. PMID:26196901

  11. Organic Farming and Landscape Structure: Effects on Insect-Pollinated Plant Diversity in Intensively Managed Grasslands

    PubMed Central

    Power, Eileen F.; Kelly, Daniel L.; Stout, Jane C.

    2012-01-01

    Parallel declines in insect-pollinated plants and their pollinators have been reported as a result of agricultural intensification. Intensive arable plant communities have previously been shown to contain higher proportions of self-pollinated plants compared to natural or semi-natural plant communities. Though intensive grasslands are widespread, it is not known whether they show similar patterns to arable systems nor whether local and/or landscape factors are influential. We investigated plant community composition in 10 pairs of organic and conventional dairy farms across Ireland in relation to the local and landscape context. Relationships between plant groups and local factors (farming system, position in field and soil parameters) and landscape factors (e.g. landscape complexity) were investigated. The percentage cover of unimproved grassland was used as an inverse predictor of landscape complexity, as it was negatively correlated with habitat-type diversity. Intensive grasslands (organic and conventional) contained more insect-pollinated forbs than non-insect pollinated forbs. Organic field centres contained more insect-pollinated forbs than conventional field centres. Insect-pollinated forb richness in field edges (but not field centres) increased with increasing landscape complexity (% unimproved grassland) within 1, 3, 4 and 5km radii around sites, whereas non-insect pollinated forb richness was unrelated to landscape complexity. Pollination systems within intensive grassland communities may be different from those in arable systems. Our results indicate that organic management increases plant richness in field centres, but that landscape complexity exerts strong influences in both organic and conventional field edges. Insect-pollinated forb richness, unlike that for non-insect pollinated forbs, showed positive relationships to landscape complexity reflecting what has been documented for bees and other pollinators. The insect-pollinated forbs, their

  12. Insects as a Nitrogen Source for Plants

    PubMed Central

    Behie, Scott W.; Bidochka, Michael J.

    2013-01-01

    Many plants have evolved adaptations in order to survive in low nitrogen environments. One of the best-known adaptations is that of plant symbiosis with nitrogen-fixing bacteria; this is the major route by which nitrogen is incorporated into plant biomass. A portion of this plant-associated nitrogen is then lost to insects through herbivory, and insects represent a nitrogen reservoir that is generally overlooked in nitrogen cycles. In this review we show three specialized plant adaptations that allow for the recovery of insect nitrogen; that is, plants gaining nitrogen from insects. First, we show specialized adaptations by carnivorous plants in low nitrogen habitats. Insect carnivorous plants such as pitcher plants and sundews (Nepenthaceae/Sarraceniaceae and Drosera respectively) are able to obtain substantial amounts of nitrogen from the insects that they capture. Secondly, numerous plants form associations with mycorrhizal fungi that can provide soluble nitrogen from the soil, some of which may be insect-derived nitrogen, obtained from decaying insects or insect frass. Finally, a specialized group of endophytic, insect-pathogenic fungi (EIPF) provide host plants with insect-derived nitrogen. These soil-inhabiting fungi form a remarkable symbiosis with certain plant species. They can infect a wide range of insect hosts and also form endophytic associations in which they transfer insect-derived nitrogen to the plant. Root colonizing fungi are found in disparate fungal phylogenetic lineages, indicating possible convergent evolutionary strategies between taxa, evolution potentially driven by access to carbon-containing root exudates. PMID:26462427

  13. Insects as a Nitrogen Source for Plants.

    PubMed

    Behie, Scott W; Bidochka, Michael J

    2013-01-01

    Many plants have evolved adaptations in order to survive in low nitrogen environments. One of the best-known adaptations is that of plant symbiosis with nitrogen-fixing bacteria; this is the major route by which nitrogen is incorporated into plant biomass. A portion of this plant-associated nitrogen is then lost to insects through herbivory, and insects represent a nitrogen reservoir that is generally overlooked in nitrogen cycles. In this review we show three specialized plant adaptations that allow for the recovery of insect nitrogen; that is, plants gaining nitrogen from insects. First, we show specialized adaptations by carnivorous plants in low nitrogen habitats. Insect carnivorous plants such as pitcher plants and sundews (Nepenthaceae/Sarraceniaceae and Drosera respectively) are able to obtain substantial amounts of nitrogen from the insects that they capture. Secondly, numerous plants form associations with mycorrhizal fungi that can provide soluble nitrogen from the soil, some of which may be insect-derived nitrogen, obtained from decaying insects or insect frass. Finally, a specialized group of endophytic, insect-pathogenic fungi (EIPF) provide host plants with insect-derived nitrogen. These soil-inhabiting fungi form a remarkable symbiosis with certain plant species. They can infect a wide range of insect hosts and also form endophytic associations in which they transfer insect-derived nitrogen to the plant. Root colonizing fungi are found in disparate fungal phylogenetic lineages, indicating possible convergent evolutionary strategies between taxa, evolution potentially driven by access to carbon-containing root exudates. PMID:26462427

  14. COASTAL WETLAND INSECT COMMUNITIES ALONG A TROPHIC GRADIENT IN GREEN BAY, LAKE MICHIGAN

    EPA Science Inventory

    Insects of Great Lakes coastal wetlands have received little attention in spite of their importance in food webs and sensitivity to anthropogenic stressors. We characterized insect communities from four coastal wetlands that spanned the length of a trophic gradient in Green Bay d...

  15. Effects of temporal variation in temperature and density dependence on insect population dynamics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Understanding effects of environmental variation on insect populations is important in light of predictions about increasing future climatic variability. In order to understand the effects of changing environmental variation on population dynamics and life history evolution in insects one would need...

  16. Agriculture and forestry: Identification, vigor, and disease

    NASA Technical Reports Server (NTRS)

    Jenkins, D. W.

    1972-01-01

    The agricultural and forestry areas which comprise the watershed of the Chesapeake Bay are described. Major problems of watershed creation and management with emphasis on the erosion problem are discussed. Remote sensing as it relates to the identification of plant species and vigor, pollution, disease, and insect infestation are examined. The application of infrared photography, multispectral sensing, and sequential survey is recommended to identify ecological changes and improve resources management.

  17. Agricultural Meteorology in China.

    NASA Astrophysics Data System (ADS)

    Rosenberg, Norman J.

    1982-03-01

    During nearly five weeks in China (May-June 1981), the author visited scientific institutions and experiment stations engaged in agricultural meterology and climatology research and teaching. The facilities, studies, and research programs at each institution are described and the scientific work in these fields is evaluated. Agricultural meteorology and climatology are faced with some unique problems and opportunities in China and progress in these fields may be of critical importance to that nation in coming years. The author includes culinary notes and comments on protocol in China.

  18. Thermodynamics Constrains Allometric Scaling of Optimal Development Time in Insects

    PubMed Central

    Dillon, Michael E.; Frazier, Melanie R.

    2013-01-01

    Development time is a critical life-history trait that has profound effects on organism fitness and on population growth rates. For ectotherms, development time is strongly influenced by temperature and is predicted to scale with body mass to the quarter power based on 1) the ontogenetic growth model of the metabolic theory of ecology which describes a bioenergetic balance between tissue maintenance and growth given the scaling relationship between metabolism and body size, and 2) numerous studies, primarily of vertebrate endotherms, that largely support this prediction. However, few studies have investigated the allometry of development time among invertebrates, including insects. Abundant data on development of diverse insects provides an ideal opportunity to better understand the scaling of development time in this ecologically and economically important group. Insects develop more quickly at warmer temperatures until reaching a minimum development time at some optimal temperature, after which development slows. We evaluated the allometry of insect development time by compiling estimates of minimum development time and optimal developmental temperature for 361 insect species from 16 orders with body mass varying over nearly 6 orders of magnitude. Allometric scaling exponents varied with the statistical approach: standardized major axis regression supported the predicted quarter-power scaling relationship, but ordinary and phylogenetic generalized least squares did not. Regardless of the statistical approach, body size alone explained less than 28% of the variation in development time. Models that also included optimal temperature explained over 50% of the variation in development time. Warm-adapted insects developed more quickly, regardless of body size, supporting the “hotter is better” hypothesis that posits that ectotherms have a limited ability to evolutionarily compensate for the depressing effects of low temperatures on rates of biological processes

  19. Multilevel selection and social evolution of insect societies

    NASA Astrophysics Data System (ADS)

    Korb, Judith; Heinze, Jürgen

    How sterile, altruistic worker castes have evolved in social insects and how they are maintained have long been central topics in evolutionary biology. With the advance of kin selection theory, insect societies, in particular those of haplodiploid bees, ants, and wasps, have become highly suitable model systems for investigating the details of social evolution and recently also how within-group conflicts are resolved. Because insect societies typically do not consist of clones, conflicts among nestmates arise, for example about the partitioning of reproduction and the allocation of resources towards male and female sexuals. Variation in relatedness among group members therefore appears to have a profound influence on the social structure of groups. However, insect societies appear to be remarkably robust against such variation: division of labor and task allocation are often organized in more or less the same way in societies with high as in those with very low nestmate relatedness. To explain the discrepancy between predictions from kin structure and empirical data, it was suggested that constraints-such as the lack of power or information-prevent individuals from pursuing their own selfish interests. Applying a multilevel selection approach shows that these constraints are in fact group-level adaptation preventing or resolving intracolonial conflict. The mechanisms of conflict resolution in insect societies are similar to those at other levels in the biological hierarchy (e.g., in the genome or multicellular organisms): alignment of interests, fair lottery, and social control. Insect societies can thus be regarded as a level of selection with novelties that provide benefits beyond the scope of a solitary life. Therefore, relatedness is less important for the maintenance of insect societies, although it played a fundamental role in their evolution.

  20. Topological and Functional Characterization of an Insect Gustatory Receptor

    PubMed Central

    Zhang, Hui-Jie; Anderson, Alisha R.; Trowell, Stephen C.; Luo, A-Rong; Xiang, Zhong-Huai; Xia, Qing-You

    2011-01-01

    Insect gustatory receptors are predicted to have a seven-transmembrane structure and are distantly related to insect olfactory receptors, which have an inverted topology compared with G-protein coupled receptors, including mammalian olfactory receptors. In contrast, the topology of insect gustatory receptors remains unknown. Except for a few examples from Drosophila, the specificity of individual insect gustatory receptors is also unknown. In this study, the total number of identified gustatory receptors in Bombyx mori was expanded from 65 to 69. BmGr8, a silkmoth gustatory receptor from the sugar receptor subfamily, was expressed in insect cells. Membrane topology studies on BmGr8 indicate that, like insect olfactory receptors, it has an inverted topology relative to G protein-coupled receptors. An orphan GR from the bitter receptor family, BmGr53, yielded similar results. We infer, from the finding that two distantly related BmGrs have an intracellular N-terminus and an odd number of transmembrane spans, that this is likely to be a general topology for all insect gustatory receptors. We also show that BmGr8 functions independently in Sf9 cells and responds in a concentration-dependent manner to the polyalcohols myo-inositol and epi-inositol but not to a range of mono- and di-saccharides. BmGr8 is the first chemoreceptor shown to respond specifically to inositol, an important or essential nutrient for some Lepidoptera. The selectivity of BmGr8 responses is consistent with the known responses of one of the gustatory receptor neurons in the lateral styloconic sensilla of B. mori, which responds to myo-inositol and epi-inositol but not to allo-inositol. PMID:21912618