Science.gov

Sample records for airborne pollen counts

  1. Relationships among indoor, outdoor, and personal airborne Japanese cedar pollen counts.

    PubMed

    Yamamoto, Naomichi; Matsuki, Yuuki; Yokoyama, Hiromichi; Matsuki, Hideaki

    2015-01-01

    Japanese cedar pollinosis (JCP) is an important illness caused by the inhalation of airborne allergenic cedar pollens, which are dispersed in the early spring throughout the Japanese islands. However, associations between pollen exposures and the prevalence or severity of allergic symptoms are largely unknown, due to a lack of understanding regarding personal pollen exposures in relation to indoor and outdoor concentrations. This study aims to examine the relationships among indoor, outdoor, and personal airborne Japanese cedar pollen counts. We conducted a 4-year monitoring campaign to quantify indoor, outdoor, and personal airborne cedar pollen counts, where the personal passive settling sampler that has been previously validated against a volumetric sampler was used to count airborne pollen grains. A total of 256 sets of indoor, outdoor, and personal samples (768 samples) were collected from 9 subjects. Medians of the seasonally-integrated indoor-to-outdoor, personal-to-outdoor, and personal-to-indoor ratios of airborne pollen counts measured for 9 subjects were 0.08, 0.10, and 1.19, respectively. A greater correlation was observed between the personal and indoor counts (r = 0.89) than between the personal and outdoor counts (r = 0.71), suggesting a potential inaccuracy in the use of outdoor counts as a basis for estimating personal exposures. The personal pollen counts differed substantially among the human subjects (49% geometric coefficient of variation), in part due to the variability in the indoor counts that have been found as major determinants of the personal pollen counts. The findings of this study highlight the need for pollen monitoring in proximity to human subjects to better understand the relationships between pollen exposures and the prevalence or severity of pollen allergy. PMID:26110813

  2. Relationships among Indoor, Outdoor, and Personal Airborne Japanese Cedar Pollen Counts

    PubMed Central

    Yamamoto, Naomichi; Matsuki, Yuuki; Yokoyama, Hiromichi; Matsuki, Hideaki

    2015-01-01

    Japanese cedar pollinosis (JCP) is an important illness caused by the inhalation of airborne allergenic cedar pollens, which are dispersed in the early spring throughout the Japanese islands. However, associations between pollen exposures and the prevalence or severity of allergic symptoms are largely unknown, due to a lack of understanding regarding personal pollen exposures in relation to indoor and outdoor concentrations. This study aims to examine the relationships among indoor, outdoor, and personal airborne Japanese cedar pollen counts. We conducted a 4-year monitoring campaign to quantify indoor, outdoor, and personal airborne cedar pollen counts, where the personal passive settling sampler that has been previously validated against a volumetric sampler was used to count airborne pollen grains. A total of 256 sets of indoor, outdoor, and personal samples (768 samples) were collected from 9 subjects. Medians of the seasonally-integrated indoor-to-outdoor, personal-to-outdoor, and personal-to-indoor ratios of airborne pollen counts measured for 9 subjects were 0.08, 0.10, and 1.19, respectively. A greater correlation was observed between the personal and indoor counts (r = 0.89) than between the personal and outdoor counts (r = 0.71), suggesting a potential inaccuracy in the use of outdoor counts as a basis for estimating personal exposures. The personal pollen counts differed substantially among the human subjects (49% geometric coefficient of variation), in part due to the variability in the indoor counts that have been found as major determinants of the personal pollen counts. The findings of this study highlight the need for pollen monitoring in proximity to human subjects to better understand the relationships between pollen exposures and the prevalence or severity of pollen allergy. PMID:26110813

  3. Influence of wind on daily airborne pollen counts in Catalonia (NE Iberian Peninsula)

    NASA Astrophysics Data System (ADS)

    tareq Majeed, Husam; Periago, Cristina; Alarcón, Marta; De Linares, Concepción; Belmonte, Jordina

    2016-04-01

    The aim of this study is to analize the influence of wind (speed and direction) on the daily airborne pollen counts recorded in Catalonia (NE Iberian Peninsula) of 21 pollen taxa recorded at 6 aerobiological stations: Barcelona, Bellaterra, Girona, Lleida Manresa, and Tarragona for the period 2004-2014. The taxa studied are Alnus, Betula, Castanea, Cupressaceae, Fagus, Fraxinus, Olea, Pinus, Platanus, total Quercus, Quercus deciduous type, Quercus evergreen type, Ulmus, Corylus, Pistacia, Artemisia, Chenopodiaceae/Amaranthaceae, Plantago, Poaceae, Polygonaceae, and Urticaceae. The mean daily wind direction was divided into 8 sectors: N, NE, E, SE, S, SW, W and NW. For each sector, the correlation between the daily pollen concentrations and wind speed using Spearman's rank correlation coefficient was computed and compared with the wind rose charts. The results showed that Tarragona was the station with more significant correlations followed by Bellaterra, Lleida and Manresa. On the other hand, Artemisia was the most correlated taxon with mainly negative values, and Fagus was the least. The W wind direction showed the largest number of significant correlations, mostly positive, while the N direction was the least and negatively correlated.

  4. Seasonal variations of airborne pollen in Allahabad, India.

    PubMed

    Sahney, Manju; Chaurasia, Swati

    2008-01-01

    Using a Burkard 7-day volumetric sampler a survey of airborne pollen grains in Allahabad was carried out from December 2004--November 2005 to assess the qualitative and quantitative occurrence of pollen grains during different months of the year, and to characterize the pollen seasons of dominant pollen types in the atmosphere of Allahabad. 80 pollen types were identified out of the total pollen catch of 3,416.34 pollen grains/m(3). Bulk of the pollen originated from anemophilous trees and grasses. Thirteen pollen types recorded more than 1 % of the annual total pollen catch. Holoptelea integrifolia formed the major component of the pollen spectrum constituting 46.21 % of the total pollen catch followed by Poaceae, Azadirachta indica, Ailanthus excelsa, Putranjiva roxburghii, Parthenium hysterophorus, Ricinus communis, Brassica compestris, Amaranthaceae/Chenopodiaceae, Madhuca longifolia, Syzygium cumini, other Asteraceae and Aegle marmelos. Highest pollen counts were obtained in the month of March and lowest in July. The pollen types recorded marked the seasonal pattern of occurrence in the atmosphere. February-May was the principal pollen season with maximum number of pollen counts and pollen types. Chief sources of pollen during this period were arboreal taxa. September-October was the second pollen season with grasses being the main source of pollen. Airborne pollen spectrum reflected the vegetation of Allahabad, except for Alnus sp., which grows in the Himalayan region. A significant negative correlation was found of daily pollen counts with minimum temperature, relative humidity and rainfall. PMID:19061265

  5. A Simple Method for Collecting Airborne Pollen

    ERIC Educational Resources Information Center

    Kevan, Peter G.; DiGiovanni, Franco; Ho, Rong H.; Taki, Hisatomo; Ferguson, Kristyn A.; Pawlowski, Agata K.

    2006-01-01

    Pollination is a broad area of study within biology. For many plants, pollen carried by wind is required for successful seed set. Airborne pollen also affects human health. To foster studies of airborne pollen, we introduce a simple device--the "megastigma"--for collecting pollen from the air. This device is flexible, yielding easily obtained data…

  6. Airborne pollen of allergenic herb species in Toledo (Spain).

    PubMed

    Vaquero, Consolación; Rodríguez-Torres, Alfonso; Rojo, Jesús; Pérez-Badia, Rosa

    2013-01-01

    This study analysed airborne pollen counts for allergenic herb taxa in Toledo (central Spain), a major tourist city receiving over 2 million visitors per year, located in the region of Castilla-La Mancha. The taxa selected were Chenopodiaceae-Amaranthaceae, Plantago, Poaceae and Urticaceae, all of which produce allergenic pollen giving rise to serious symptoms in pollen-allergy sufferers. Aerobiological data were recorded over a 6-year period (2005 to 2010) using the sampling and analysis procedures recommended by the Spanish Aerobiology Network. The abundance and the temporal (annual, daily and intradiurnal) distribution of these pollen types were analysed, and the influence of weather-related factors on airborne pollen counts was assessed. Pollen from herbaceous species accounted for 20.9% of total airborne pollen in Toledo, the largest contributor being Poaceae, with 8.5% of the total pollen count; this family was also the leading cause of respiratory allergies. Examination of intradiurnal variation revealed three distinct distribution patterns: (1) peak daily counts for Chenopodiaceae-Amaranthaceae and Plantago were recorded during the hottest part of the day, i.e. from 1400 to 1600 hours; (2) Urticaceae displayed two peaks (1400-1600 and 2200 hours); and (3) Poaceae counts remained fairly stable throughout the day. Two main risk periods were identified for allergies: spring, with allergies caused by Urticaceae, Plantago and Poaceae pollen, and summer, due to Chenopodiaceae-Amaranthaceae pollen. PMID:22331454

  7. A Six-Year Study on the Changes in Airborne Pollen Counts and Skin Positivity Rates in Korea: 2008–2013

    PubMed Central

    Park, Hye Jung; Lee, Jae-Hyun; Park, Kyung Hee; Kim, Kyu Rang; Han, Mae Ja; Choe, Hosoeng

    2016-01-01

    Purpose The occurrence of pollen allergy is subject to exposure to pollen, which shows regional and temporal variations. We evaluated the changes in pollen counts and skin positivity rates for 6 years, and explored the correlation between their annual rates of change. Materials and Methods We assessed the number of pollen grains collected in Seoul, and retrospectively reviewed the results of 4442 skin-prick tests conducted at the Severance Hospital Allergy-Asthma Clinic from January 1, 2008 to December 31, 2013. Results For 6 years, the mean monthly total pollen count showed two peaks, one in May and the other in September. Pollen count for grasses also showed the same trend. The pollen counts for trees, grasses, and weeds changed annually, but the changes were not significant. The annual skin positivity rates in response to pollen from grasses and weeds increased significantly over the 6 years. Among trees, the skin positivity rates in response to pollen from walnut, popular, elm, and alder significantly increased over the 6 years. Further, there was a significant correlation between the annual rate of change in pollen count and the rate of change in skin positivity rate for oak and hop Japanese. Conclusion The pollen counts and skin positivity rates should be monitored, as they have changed annually. Oak and hop Japanese, which showed a significant correlation with the annual rate of change in pollen count and the rate of change in skin positivity rate over the 6 years may be considered the major allergens in Korea. PMID:26996572

  8. A Method of Recording and Predicting the Pollen Count.

    ERIC Educational Resources Information Center

    Buck, M.

    1985-01-01

    A hair dryer, plastic funnel, and microscope slide can be used for predicting pollen counts on a day-to-day basis. Materials, methods for assembly, collection technique, meteorological influences, and daily patterns are discussed. Data collected using the apparatus suggest that airborne grass products other than pollen also affect hay fever…

  9. Models for forecasting airborne Cupressaceae pollen levels in central Spain

    NASA Astrophysics Data System (ADS)

    Sabariego, Silvia; Cuesta, Pedro; Fernández-González, Federico; Pérez-Badia, Rosa

    2012-03-01

    The influence of meteorological variables on airborne Cupressaceae pollen levels in central Spain was analyzed, and prediction models based on polynomial and multiple regressions were used to predict pollen counts throughout the pollen season. The Cupressaceae pollen type was selected in view of both its abundance in the atmosphere of the central Iberian Peninsula (particularly from January to March) and its allergenic importance. Sampling was performed uninterruptedly over a 5-year period, using a Hirst volumetric sampler and the sampling method established by the Spanish Aerobiology Network. Temperature displayed the strongest (positive) correlation with Cupressaceae pollen counts. Polynomial and multiple regression analysis showed that maximum temperature was the most influential variable included in prediction models. The prediction equations obtained for the study period were reasonably satisfactory, accounting for 48% and 59% of the variation in airborne pollen levels.

  10. Effect of land uses and wind direction on the contribution of local sources to airborne pollen.

    PubMed

    Rojo, Jesús; Rapp, Ana; Lara, Beatriz; Fernández-González, Federico; Pérez-Badia, Rosa

    2015-12-15

    The interpretation of airborne pollen levels in cities based on the contribution of the surrounding flora and vegetation is a useful tool to estimate airborne allergen concentrations and, consequently, to determine the allergy risk for local residents. This study examined the pollen spectrum in a city in central Spain (Guadalajara) and analysed the vegetation landscape and land uses within a radius of 20km in an attempt to identify and locate the origin of airborne pollen and to determine the effect of meteorological variables on pollen emission and dispersal. The results showed that local wind direction was largely responsible for changes in the concentrations of different airborne pollen types. The land uses contributing most to airborne pollen counts were urban green spaces, though only 0.1% of the total surface area studied, and broadleaved forest which covered 5% of the study area. These two types of land use together accounted for 70% of the airborne pollen. Crops, scrubland and pastureland, though covering 80% of the total surface area, contributed only 18.6% to the total pollen count, and this contribution mainly consisted of pollen from Olea and herbaceous plants, including Poaceae, Urticaceae and Chenopodiaceae-Amaranthaceae. Pollen from ornamental species were mainly associated with easterly (Platanus), southerly (Cupressaceae) and westerly (Cupressaceae and Platanus) winds from the areas where the city's largest parks and gardens are located. Quercus pollen was mostly transported by winds blowing in from holm-oak stands on the eastern edge of the city. The highest Populus pollen counts were associated with easterly and westerly winds blowing in from areas containing rivers and streams. The airborne pollen counts generally rose with increasing temperature, solar radiation and hours of sunlight, all of which favour pollen release. In contrast, pollen counts declined with increased relative humidity and rainfall, which hinder airborne pollen transport

  11. Ambrosia airborne pollen concentration modelling and evaluation over Europe

    NASA Astrophysics Data System (ADS)

    Hamaoui-Laguel, Lynda; Vautard, Robert; Viovy, Nicolas; Khvorostyanov, Dmitry; Colette, Augustin

    2014-05-01

    Native from North America, Ambrosia artemisiifolia L. (Common Ragweed) is an invasive annual weed introduced in Europe in the mid-nineteenth century. It has a very high spreading potential throughout Europe and releases very allergenic pollen leading to health problems for sensitive persons. Because of its health effects, it is necessary to develop modelling tools to be able to forecast ambrosia air pollen concentration and to inform allergy populations of allergenic threshold exceedance. This study is realised within the framework of the ATOPICA project (https://www.atopica.eu/) which is designed to provide first steps in tools and estimations of the fate of allergies in Europe due to changes in climate, land use and air quality. To calculate and predict airborne concentrations of ambrosia pollen, a chain of models has been built. Models have been developed or adapted for simulating the phenology (PMP phonological modelling platform), inter-annual production (ORCHIDEE vegetation model), release and airborne processes (CHIMERE chemical transport model) of ragweed pollen. Airborne pollens follow processes similar to air quality pollutants in CHIMERE with some adaptations. The detailed methodology, formulations and input data will be presented. A set of simulations has been performed to simulate airborne concentrations of pollens over long time periods on a large European domain. Hindcast simulations (2000 - 2012) driven by ERA-Interim re-analyses are designed to best simulate past periods airborne pollens. The modelled pollen concentrations are calibrated with observations and validated against additional observations. Then, 20-year long historical simulations (1986 - 2005) are carried out using calibrated ambrosia density distribution and climate model-driven weather in order to serve as a control simulation for future scenarios. By comparison with multi-annual observed daily pollen counts we have shown that the model captures well the gross features of the pollen

  12. Airborne pollen trends in the Iberian Peninsula.

    PubMed

    Galán, C; Alcázar, P; Oteros, J; García-Mozo, H; Aira, M J; Belmonte, J; Diaz de la Guardia, C; Fernández-González, D; Gutierrez-Bustillo, M; Moreno-Grau, S; Pérez-Badía, R; Rodríguez-Rajo, J; Ruiz-Valenzuela, L; Tormo, R; Trigo, M M; Domínguez-Vilches, E

    2016-04-15

    Airborne pollen monitoring is an effective tool for studying the reproductive phenology of anemophilous plants, an important bioindicator of plant behavior. Recent decades have revealed a trend towards rising airborne pollen concentrations in Europe, attributing these trends to an increase in anthropogenic CO2 emissions and temperature. However, the lack of water availability in southern Europe may prompt a trend towards lower flowering intensity, especially in herbaceous plants. Here we show variations in flowering intensity by analyzing the Annual Pollen Index (API) of 12 anemophilous taxa across 12 locations in the Iberian Peninsula, over the last two decades, and detecting the influence of the North Atlantic Oscillation (NAO). Results revealed differences in the distribution and flowering intensity of anemophilous species. A negative correlation was observed between airborne pollen concentrations and winter averages of the NAO index. This study confirms that changes in rainfall in the Mediterranean region, attributed to climate change, have an important impact on the phenology of plants. PMID:26803684

  13. Impact and correlation of environmental conditions on pollen counts in Karachi, Pakistan.

    PubMed

    Perveen, Anjum; Khan, Muneeba; Zeb, Shaista; Imam, Asif Ali

    2015-02-01

    A quantitative and qualitative survey of airborne pollen was performed in the city of Karachi, and the pollen counts were correlated with different climatic conditions. The aim of the study was to determine the possible effect of meteorological factors on airborne pollen distribution in the atmosphere of Karachi city. Pollen sampling was carried out by using Burkard spore Trap for the period of August 2009 to July 2010, and a total of 2,922 pollen grains/m(3) were recorded. In this survey, 22 pollen types were recognized. The highest pollen count was contributed by Poaceae pollen type (1,242 pollen grains/m(3)) followed by Amaranthaceae/Chenopodiaceae (948 pollen grains/m(3)), Cyperus rotundus (195 pollen grains/m(3)) and Prosopis juliflora (169 pollen grains/m(3)). Peak pollen season was in August showing a total of 709 pollen grains/m(3) and lowest pollen count was observed in January-2010. Pearson's chi-square test was performed for the possible correlation of pollen counts and climatic factors. The test revealed significant positive correlation of wind speed with pollen types of Amaranthaceae/Chenopodiaceae; Brassica campestris; Asteraceae; and Thuja orientalis. While the correlation of "average temperature" showed significant positive value with Asteraceae and Tamarix indica pollen types. Negative correlation was observed between humidity/ precipitation and pollen types of Brassica campestris; Daucus carota; Ephedra sp.; and Tamarix indica. In the light of above updated data one could identify various aeroallergens present in the air of Karachi city. PMID:25530143

  14. Physico-chemical characteristics of visibility impairment by airborne pollen in an urban area

    NASA Astrophysics Data System (ADS)

    Kim, Kyung W.

    The number of airborne pollen produced from plants is visible as a haze mixed with urban air pollution in an urban area when atmospheric conditions are proper for pollination of pollen from April to May in Korea. The big loading of airborne pollen can cause further visibility degradation in an urban area. In order to investigate physico-chemical characteristics of visibility impairment by airborne pollen, chemical aerosol measurements, optical aerosol monitoring, and scenic monitoring were performed during the intensive monitoring period from April 19 to May 2, 2005 in the urban area of Seoul, Korea. The particles collected on filters were examined with a scanning electron microscope (SEM) interfaced with an energy dispersive X-ray analysis to characterize size, elemental composition, and count of airborne pollen. During the airborne pollen period, the daily averaged number concentrations of airborne pollen; P and P were calculated to be 8.4±6.9 and 113.7±91.1 m -3, respectively. The daily averaged light extinction coefficient ( bext), light scattering coefficient for open path ( bscat), light scattering coefficient for dry particle in the fine regime ( bscat,fine), and light absorption coefficient in the fine regime ( babs,fine) were measured to be 459±267, 357±214, 263±165, and 44±30 Mm -1, respectively. And mass concentration of PM 2.5 and PM 10 were measured to be 46.5±29.1 and 97.0±41.7 μg m -3. The average light absorption coefficient by airborne pollen was estimated to be about 30 M m -1 and the average light scattering coefficient by airborne pollen was estimated to be 67±57 Mm -1. During the airborne pollen period the average contribution of airborne pollen to visibility impairment was roughly estimated to be 19-25%.

  15. Controlling the levels of airborne pollen: can heterogeneous photocatalysis help?

    PubMed

    Sapiña, M; Jimenez-Relinque, E; Castellote, M

    2013-10-15

    Airborne pollen is a worldwide problem because is a very important allergenic agent; it can be altered only by certain microorganisms and by some oxidizers, such as reactive oxygen species (ROS). On the other hand, heterogeneous photocatalysis (HPC) arose as a promising technology for reducing the level of contaminants in the air, based on their degradation by the production of ROS. In this paper, study of the feasibility of HPC to diminish the counts of pollen is undertaken. The research has been carried out at different levels, from solutions to mortar specimens with the evidence that HPC is able to reduce the amount of pollen grains. This is a major breakthrough that opens the door to a whole field of research, already full of gaps, whose implications could be quite controversial. PMID:24063577

  16. Pollen counts and their relationship to meteorological factors in Ankara, Turkey during 2005-2008

    NASA Astrophysics Data System (ADS)

    Kizilpinar, Ilginc; Civelek, Ersoy; Tuncer, Ayfer; Dogan, Cahit; Karabulut, Erdem; Sahiner, Umit M.; Yavuz, S. Tolga; Sackesen, Cansin

    2011-07-01

    Pollen plays an important role in the development and exacerbation of allergic diseases. We aimed to investigate the days with highest counts of the most allergenic pollens and to identify the meteorological factors affecting pollen counts in the atmosphere of Ankara, Turkey. Airborne pollen measurements were carried out from 2005 to 2008 with a Burkard volumetric 7-day spore trap. Microscope counts were converted into atmospheric concentrations and expressed as pollen grains/m3. Meteorological parameters were obtained from the State Meteorological Service. All statistical analyses were done with pollen counts obtained from March to October for each year. The percentages of tree, grass and weed pollens were 72.1% ( n = 24,923), 12.8% ( n = 4,433) and 15.1% ( n = 5,219), respectively. The Pinaceae family from tree taxa (39% to 57%) and the Chenopodiaceae/Amaranthaceae family from weed taxa, contributed the highest percentage of pollen (25% to 43%), while from the grass taxa, only the Poaceae family was detected from 2005 to 2008. Poaceae and Chenopodiaceae/Amaranthaceae families, which are the most allergenic pollens, were found in high numbers from May to August in Ankara. In multiple logistic regression analysis, wind speed (OR = 1.18, CI95% = 1.02-1.36, P = 0.023) for tree pollen, daily mean temperature (OR = 1.10, CI95% = 1.04-1.17, P = 0.001) and sunshine hours (OR = 1.15, CI95% = 1.01-1.30, P = 0.033) for grass pollen, and sunshine hours (OR = 3.79, CI95% = 1.03-13.92, P = 0.044) for weed pollen were found as significant risk factors for high pollen count. The pollen calendar and its association with meteorological factors depend mainly on daily temperature, sunshine hours and wind speed, which may help draw the attention of physicians and allergic patients to days with high pollen counts.

  17. Characterisation of the airborne pollen spectrum in Guadalajara (central Spain) and estimation of the potential allergy risk.

    PubMed

    Rojo, Jesús; Rapp, Ana; Lara, Beatriz; Sabariego, Silvia; Fernández-González, Federico; Pérez-Badia, Rosa

    2016-03-01

    Aerobiological research into airborne pollen diversity and seasonal variations in pollen counts has become increasingly important over recent decades due to the growing incidence of asthma, rhinitis and other pollen-related allergic conditions. Airborne pollen in Guadalajara (Castilla-La Mancha, Spain) was studied over a 6-year period (2008-2013) using a Hirst-type volumetric spore trap. The highest pollen concentrations were recorded from February to June, coinciding with the pollen season of the pollen types that most contribute to the local airborne pollen spectrum: Cupressaceae (32.2%), Quercus (15.1%), Platanus (13.2%), Olea (8.3%), Populus (7.8%) and Poaceae (7.2%). These are therefore critical months for allergy sufferers. The pollen calendar was typically Mediterranean and comprised 25 pollen types. Between January and March, Cupressaceae pollen concentrations exceeded allergy risk thresholds on 38 days. Other woody species such as Olea and Platanus have a shorter pollen season, and airborne concentrations exceeded allergy risk thresholds on around 13 days in each case. Poaceae pollen concentrations attained allergy risk levels on 26 days between May and July. Other highly allergenic pollen types included Urticaceae and Chenopodiaceae-Amaranthaceae, though these are less abundant than other pollen types in Guadalajara and did not exceed risk thresholds on more than 3 and 5 days, respectively. PMID:26832913

  18. Quantitative DNA Analyses for Airborne Birch Pollen

    PubMed Central

    Müller-Germann, Isabell; Vogel, Bernhard; Vogel, Heike; Pauling, Andreas; Fröhlich-Nowoisky, Janine; Pöschl, Ulrich; Després, Viviane R.

    2015-01-01

    Birch trees produce large amounts of highly allergenic pollen grains that are distributed by wind and impact human health by causing seasonal hay fever, pollen-related asthma, and other allergic diseases. Traditionally, pollen forecasts are based on conventional microscopic counting techniques that are labor-intensive and limited in the reliable identification of species. Molecular biological techniques provide an alternative approach that is less labor-intensive and enables identification of any species by its genetic fingerprint. A particularly promising method is quantitative Real-Time polymerase chain reaction (qPCR), which can be used to determine the number of DNA copies and thus pollen grains in air filter samples. During the birch pollination season in 2010 in Mainz, Germany, we collected air filter samples of fine (<3 μm) and coarse air particulate matter. These were analyzed by qPCR using two different primer pairs: one for a single-copy gene (BP8) and the other for a multi-copy gene (ITS). The BP8 gene was better suitable for reliable qPCR results, and the qPCR results obtained for coarse particulate matter were well correlated with the birch pollen forecasting results of the regional air quality model COSMO-ART. As expected due to the size of birch pollen grains (~23 μm), the concentration of DNA in fine particulate matter was lower than in the coarse particle fraction. For the ITS region the factor was 64, while for the single-copy gene BP8 only 51. The possible presence of so-called sub-pollen particles in the fine particle fraction is, however, interesting even in low concentrations. These particles are known to be highly allergenic, reach deep into airways and cause often severe health problems. In conclusion, the results of this exploratory study open up the possibility of predicting and quantifying the pollen concentration in the atmosphere more precisely in the future. PMID:26492534

  19. Determination of allergenic load and pollen count of Cupressus arizonica pollen by flow cytometry using Cup a1 polyclonal antibody.

    PubMed

    Benítez, Francisco Moreno; Camacho, Antonio Letrán; Del Cuvillo Bernal, Alfonso; de Medina, Pedro Lobatón Sánchez; Cózar, Francisco J García; Romeu, Ma Luisa Espinazo

    2013-07-10

    Background: There is an increase in the incidence of pollen related allergy, thus information on pollen schedules would be a great asset for physicians to improve the clinical care of patients. Like cypress pollen sensitization shows a high prevalence among the causes of allergic rhinitis, and therefore it is of interest to use it like a model of study, distinguishing cypress pollen, pollen count and allergenic load level. In this work, we use a flow cytometry based technique to obtain both Cupressus arizonica pollen count and allergenic load, using specific rabbit polyclonal antibody Cup a1 and its comparison with optical microscopy technique measurement. Methods: Airborne samples were collected from Burkard Spore-Trap and Burkard Cyclone Cupressus arizonica pollen was studied using specific rabbit polyclonal antibody Cup a1, labelled with AlexaFluor(®) 488 or 750 and analysed by Flow Cytometry in both an EPICS XL and Cyan ADP cytometers (Beckman Coulter(®) ). Optical microscopy study was realized with a Leica optical microscope. Bland & Altman was used to determine agreement between both techniques measured. Results: We can identify three different populations based on rabbit polyclonal antibody Cup a1 staining. The main region (44.5%) had 97.3% recognition, a second region (25%) with 28% and a third region (30.5%) with 68% respectively. Immunofluorescence and confocal microscopy showed that main region corresponds to whole pollen grains, the second region are pollen without exine and the third region is constituted by smaller particles with allergenic properties. Pollen schedule shows a higher correlation measured by optical microscopy and flow cytometry in the pollen count with a p-value: 0.0008E(-2) and 0.0002 with regard to smaller particles, so the Bland & Altman measurement showed a good correlation between them, p-value: 0,0003. Conclusion: Determination of pollen count and allergenic load by flow cytometry represents an important tool in the

  20. Wavelet-based fractal analysis of airborne pollen

    NASA Astrophysics Data System (ADS)

    Degaudenzi, M. E.; Arizmendi, C. M.

    1999-06-01

    The most abundant biological particles in the atmosphere are pollen grains and spores. Self-protection of a pollen allergy is possible through information about future pollen contents in the air. In spite of the importance of airborne pollen concentration forecasting, it has not been possible to predict the pollen concentrations with great accuracy, and about 25% of daily pollen forecasts result in failures. Previous analyses of the dynamic characteristics of atmospheric pollen time series indicate that the system can be described by a low dimensional chaotic map. We apply a wavelet transform to study the multifractal characteristics of an airborne pollen time series. The information and the correlation dimensions correspond to a chaotic system showing a loss of information with time evolution.

  1. On the causes of variability in amounts of airborne grass pollen in Melbourne, Australia

    NASA Astrophysics Data System (ADS)

    de Morton, Julian; Bye, John; Pezza, Alexandre; Newbigin, Edward

    2011-07-01

    In Melbourne, Australia, airborne grass pollen is the predominant cause of hay fever (seasonal rhinitis) during late spring and early summer, with levels of airborne grass pollen also influencing hospital admissions for asthma. In order to improve predictions of conditions that are potentially hazardous to susceptible individuals, we have sought to better understand the causes of diurnal, intra-seasonal and inter-seasonal variability of atmospheric grass pollen concentrations (APC) by analysing grass pollen count data for Melbourne for 16 grass pollen seasons from 1991 to 2008 (except 1994 and 1995). Some of notable features identified in this analysis were that on days when either extreme (>100 pollen grains m-3) or high (50-100 pollen grains m-3) levels of grass pollen were recorded the winds were of continental origin. In contrast, on days with a low (<20 pollen grains m-3) concentration of grass pollen, winds were of maritime origin. On extreme and high grass pollen days, a peak in APC occurred on average around 1730 hours, probably due to a reduction in surface boundary layer turbulence. The sum of daily APC for each grass pollen season was highly correlated ( r = 0.79) with spring rainfall in Melbourne for that year, with about 60% of a declining linear trend across the study period being attributable to a reduction of meat cattle and sheep (and hence grazing land) in rural areas around Melbourne. Finally, all of the ten extreme pollen events (3 days or more with APC > 100 pollen grains m-3) during the study period were characterised by an average downward vertical wind anomaly in the surface boundary layer over Melbourne. Together these findings form a basis for a fine resolution atmospheric general circulation model for grass pollen in Melbourne's air that can be used to predict daily (and hourly) APC. This information will be useful to those sectors of Melbourne's population that suffer from allergic problems.

  2. [A relationship between birch pollen counts and meteorological factors in Sapporo].

    PubMed

    Shirasaki, Hideaki; Yamamoto, Tetsuo; Saikawa, Etsuko; Seki, Nobuhiko; Asakura, Kohji; Kataura, Akikatsu; Himi, Tetsuo

    2014-05-01

    Occurrence of airborne pollen in Sapporo has been studied for 19 years during the period between 1995 and 2013. There are wide year-to-year variations in the quantities of birch pollens. A simple linear regression with the least squares method was used for studying correlations between the annual quantities of birch pollens and the meteorological factors. A significant positive correlation was found between the hours of sunlight in June of the preceding year and the annual birch pollen concentrations with the correlation coefficient, R = 0.667. Also, we found the significant positive correlation between the hours of sunlight in March and the annual birch pollen concentrations with the correlation coefficient, R = 0.684. These results suggest that the atmospheric birch pollen counts can be predicted from meteorological factors. PMID:24956742

  3. An electronic pollen detection method using Coulter counting principle

    NASA Astrophysics Data System (ADS)

    Zhang, Zheng; Zhe, Jiang; Chandra, Santanu; Hu, Jun

    A method for detecting and counting pollen particles based on Coulter counting principle is presented. This approach also provides information on the size and surface charges of the micro particles, allowing for preliminary differentiation of pollens from other micro particles. Three samples are studied: polymethyl methacrylate particles, tree pollens from Juniperus Scopulorum and grass pollens from Secale Cerale. The samples, suspended in diluted KCl aqueous solutions in an electrochemical cell, were allowed to pass through a microchannel and the conductance of the microchannel was sampled with a Gamry ® Potentiostat. The changes in the conductance due to the passing of the micro particles was thus recorded and analyzed. The experimental results showed that tree pollens and grass pollens display distinctive behaviors. The phenomena may be attributed to the differences in the surface characteristics of the pollens and is potentially useful for counting and differentiating different micro particles.

  4. Detection of airborne allergen (Pla a 1) in relation to Platanus pollen in Córdoba, South Spain.

    PubMed

    Alcázar, Purificación; Galán, Carmen; Torres, Carmen; Domínguez-Vilches, Eugenio

    2015-01-01

    Córdoba is one of the Spanish cities with the highest records of plane tree pollen grains in the air. Clinical studies have identified Platanus as a major cause of pollinosis. This fact provokes an important public health problem during early spring when these trees bloom. The objective of the study is to evaluate the correlation between airborne pollen counts and Pla a 1 aeroallergen concentrations in Córdoba, to elucidate if airborne pollen can be an accurate measure that helps to explain the prevalence of allergenic symptoms. Pollen sampling was performed during 2011-2012 using a Hirst-type sampler. Daily average concentration of pollen grains (pollen grains/m 3 ) was obtained following the methodology proposed by the Spanish Aerobiology Network. A multi-vial cyclone was used for the aeroallergen quantification. Allergenic particles were measured by ELISA using specific antibodies Pla a 1. The trend of Platanus pollen was characterized by a marked seasonality, reaching high concentrations in a short period of time. Airborne pollen and aeroallergen follow similar trends. The overlapping profile between both variables during both years shows that pollen and Pla a 1 are significantly correlated. The highest significant correlation coefficients were obtained during 2011 and for the post peak. Although some studies have found notable divergence between pollen and allergen concentrations in the air, in the case of Platanus in Córdoba, similar aerobiological dynamics between pollen and Pla a 1 have been found. Allergenic activity was found only during the plane tree pollen season, showing a close relationship with daily pollen concentrations. The obtained pollen potency was similar for both years of study. The results suggest that the allergenic response in sensitive patients to plane tree pollen coincide with the presence and magnitude of airborne pollen. PMID:25780836

  5. Meteorological variables connected with airborne ragweed pollen in Southern Hungary.

    PubMed

    Makra, L; Juhász, M; Borsos, E; Béczi, R

    2004-09-01

    About 30% of the Hungarian population has some type of allergy, 65% of them have pollen sensitivity, and at least 60% of this pollen sensitivity is caused by ragweed. The short (or common) ragweed (Ambrosia artemisiifolia = Ambrosia elatior) has the most aggressive pollen of all. Clinical investigations prove that its allergenic pollen is the main reason for the most massive, most serious and most long-lasting pollinosis. The air in the Carpathian Basin is the most polluted with ragweed pollen in Europe. The aim of the study is to analyse how ragweed pollen concentration is influenced by meteorological elements in a medium-sized city, Szeged, Southern Hungary. The data basis consists of daily ragweed pollen counts and averages of 11 meteorological parameters for the 5-year daily data set, between 1997 and 2001. The study considers some of the ragweed pollen characteristics for Szeged. Application of the Makra test indicates the same period for the highest pollen concentration as that established by the main pollination period. After performing factor analysis for the daily ragweed pollen counts and the 11 meteorological variables examined, four factors were retained that explain 84.4% of the total variance of the original 12 variables. Assessment of the daily pollen number was performed by multiple regression analysis and results based on deseasonalised and original data were compared. PMID:15103548

  6. Characterisation of particulate matter on airborne pollen grains.

    PubMed

    Ribeiro, Helena; Guimarães, Fernanda; Duque, Laura; Noronha, Fernando; Abreu, Ilda

    2015-11-01

    A characterization of the physical-chemical composition of the atmospheric PM adsorbed to airborne pollen was performed. Airborne pollen was sampled using a Hirst-type volumetric spore sampler and observed using a Field Emission Electron Probe Microanalyser for PM analysis. A secondary electron image was taken of each pollen grain and EDS spectra were obtained for individually adsorbed particles. All images were analysed and the size parameters of the particles adsorbed to pollen was determined. The measured particles' equivalent diameter varied between 0.1 and 25.8 μm, mostly in the fine fraction. The dominant particulates identified were Si-rich, Organic-rich, SO-rich, Metals & Oxides and Cl-rich. Significant daily differences were observed in the physical-chemical characteristics of particles adsorbed to the airborne pollen wall. These differences were correlated with weather parameters and atmospheric PM concentration. Airborne pollen has the ability to adsorb fine particles that may enhance its allergenicity. PMID:26141127

  7. Impact of land cover changes and climate on the main airborne pollen types in Southern Spain.

    PubMed

    García-Mozo, Herminia; Oteros, Jose Antonio; Galán, Carmen

    2016-04-01

    Airborne pollen concentrations strongly correlate with flowering intensity of wind-pollinated species growing at and around monitoring sites. The pollen spectrum, and the variations in its composition and concentrations, is influenced by climatic features and by available nutritional resources but it is also determined by land use and its changes. The first factor influence is well known on aerobiological researches but the impact of land cover changes has been scarcely studied until now. This paper reports on a study carried out in Southern Spain (Córdoba city) examining airborne pollen trends over a 15-year period and it explores the possible links both to changes in land use and to climate variations. The Seasonal-Trend Decomposition procedure based on Loess (STL) which decomposes long-term data series into smaller seasonal component patterns was applied. Trends were compared with recorded changes in land use at varying distances from the city in order to determine their possible influence on pollen-count variations. The influence of climate-related factors was determined by means of non-parametric correlation analysis. The STL method proved highly effective for extracting trend components from pollen time series, because their features vary widely and can change quickly in a short term. Results revealed mixed trends depending on the taxa and reflecting fluctuations in land cover and/or climate. A significant rising trend in Olea pollen counts was observed, attributable both to the increasing olive-growing area but also to changes in temperature and rainfall. Poaceae pollen concentrations also increased, due largely to an expansion of heterogeneous agricultural areas and to an increase in pollen season length positively influenced by rainfall and temperature. By contrast, the significant declining trend observed for pollen from ruderal taxa, such as Amaranthaceae, Rumex, Plantago and Urticaceae, may be linked to changes in urban planning strategies with a

  8. Analysis of airborne pollen concentrations in Zagreb, Croatia, 2002.

    PubMed

    Peternel, Renata; Culig, Josip; Mitić, Bozena; Vukusić, Ivan; Sostar, Zvonimir

    2003-01-01

    Employing the volumetric method by use of a Hirst sampler, a total of 71,286 pollen grains, as many as 94.20% of them allergenic, were recorded in the air samples from the city of Zagreb during the 2002 pollen season. Among identified pollen of 35 plant species/genera/families, 23 were allergenic: Taxus/Juniperus, Alnus sp., Fraxinus sp., Betula sp., Corylus sp., Poaceae, Urticaceae, Artemisia sp., Ambrosia sp., Carpinus sp., Castanea sp., Chenopodiaceae, Salix sp., Populus sp., Ulmus sp., Juglans sp., Quercus sp., Platanus sp., Fagus sp., Plantago sp., Pinus sp., Picea sp. and Abies sp. The pollen of these plants also cause the majority of pollinosis in Europe. Study results and the pollen calendar designed for the 2002 pollen season for the City of Zagreb provide useful data for allergologists to reach an accurate diagnosis. The calendar also provides timely information on airborne pollen types and air concentrations for individuals with pollen hypersensitivity, thus allowing them to adjust their daily activities so as to minimize their contact with allergens and improve their quality of life both at home and at work. PMID:12852741

  9. Identification of potential sources of airborne Olea pollen in the Southwest Iberian Peninsula

    NASA Astrophysics Data System (ADS)

    Fernández-Rodríguez, S.; Ambelas Skjøth, C.; Tormo-Molina, R.; Brandao, R.; Caeiro, E.; Silva-Palacios, I.; Gonzalo-Garijo, Á.; Smith, M.

    2012-04-01

    This study aims to determine the potential origin of Olea pollen recorded in Badajoz in the Southwest of the Iberian Peninsula during 2009-2011. This was achieved using a combination of daily average and diurnal (hourly) airborne Olea pollen counts recorded at Badajoz (southwestern Spain) and Évora (southeastern Portugal), an inventory of olive groves in the studied area and air mass trajectory calculations computed using the HYSPLIT model. Examining olive pollen episodes at Badajoz that had distinctly different diurnal cycles in olive pollen in relation to the mean, allowed us to identify three different scenarios where olive pollen can be transported to the city from either distant or nearby sources. Back trajectory analysis showed that olive pollen can be transported to Badajoz from the West on prevailing winds, either directly or on slow moving air masses, and from high densities of olive groves situated to the Southeast (e.g. Andalucía). Regional scale transport of olive pollen can result in increased nighttime concentrations of this important aeroallergen. This could be particularly important in Mediterranean countries where people can be outdoors during this time due to climate and lifestyle. Such studies are valuable for allergy sufferers and health care professionals because the information can be incorporated into forecasts, the outputs of which are used for avoiding exposure to aeroallergens and planning medication. The results of studies of this nature can also be used for examining gene flow in this important agricultural crop.

  10. Total pollen counts do not influence active surface measurements

    NASA Astrophysics Data System (ADS)

    Moshammer, Hanns; Schinko, Herwig; Neuberger, Manfred

    We investigated the temporal association of various aerosol parameters with pollen counts in the pollen season (April 2001) in Linz, Austria. We were especially interested in the relationship between active surface (or Fuchs' surface) because we had shown previously (Atmos. Environ. 37 (2003) 1737-1744) that this parameter during the same observation period was a better predictor for acute respiratory symptoms in school children (like wheezing, shortness of breath, and cough) and reduced lung function on the same day than particle mass (PM 10). While active surface is most sensitive for fine particles with a diameter of less than 100 nm it has no strict upper cut-off regarding particle size and so could eventually be influenced also by larger particles if their numbers were high. All particle mass parameters tested (TSP, PM 10, PM 1) were weakly ( r approximately 0.2) though significantly correlated with pollen counts but neither was active surface nor total particle counts (CPC). The weak association of particle mass and pollen counts was due mainly to similar diurnal variations and a linear trend over time. Only the mass of the coarse fraction (TSP minus PM 10) remained associated with pollen counts significantly after controlling for these general temporal patterns.

  11. Pla a_1 aeroallergen immunodetection related to the airborne Platanus pollen content.

    PubMed

    Fernández-González, M; Guedes, A; Abreu, I; Rodríguez-Rajo, F J

    2013-10-01

    Platanus hispanica pollen is considered an important source of aeroallergens in many Southern European cities. This tree is frequently used in urban green spaces as ornamental specie. The flowering period is greatly influenced by the meteorological conditions, which directly affect its allergenic load in the atmosphere. The purpose of this study is to develop equations to predict the Platanus allergy risk periods as a function of the airborne pollen, the allergen concentration and the main meteorological parameters. The study was conducted by means two volumetric pollen samplers; a Lanzoni VPPS 2000 for the Platanus pollen sampling and a Burkard multivial Cyclone Sampler to collect the aeroallergen particles (Pla a_1). In addiction the Dot-Blot and the Raman spectroscopy methods were used to corroborate the results. The Pla a_1 protein is recorded in the atmosphere after the presence of the Platanus pollen, which extend the Platanus pollen allergy risk periods. The Platanus pollen and the Pla a 1 allergens concentration are associated with statistical significant variations of some meteorological variables: in a positive way with the mean and maximum temperature whereas the sign of the correlation coefficient is negative with the relative humidity. The lineal regression equation elaborated in order to forecast the Platanus pollen content in the air explain the 64.5% of variance of the pollen presence in the environment, whereas the lineal regression equation elaborated in order to forecast the aeroallergen a 54.1% of the Pla a_1 presence variance. The combination of pollen count and the allergen quantification must be assessed in the epidemiologic study of allergic respiratory diseases to prevent the allergy risk periods. PMID:23867849

  12. Airborne castanea pollen forecasting model for ecological and allergological implementation.

    PubMed

    Astray, G; Fernández-González, M; Rodríguez-Rajo, F J; López, D; Mejuto, J C

    2016-04-01

    Castanea sativa Miller belongs to the natural vegetation of many European deciduous forests prompting impacts in the forestry, ecology, allergological and chestnut food industry fields. The study of the Castanea flowering represents an important tool for evaluating the ecological conservation of North-Western Spain woodland and the possible changes in the chestnut distribution due to recent climatic change. The Castanea pollen production and dispersal capacity may cause hypersensitivity reactions in the sensitive human population due to the relationship between patients with chestnut pollen allergy and a potential cross reactivity risk with other pollens or plant foods. In addition to Castanea pollen's importance as a pollinosis agent, its study is also essential in North-Western Spain due to the economic impact of the industry around the chestnut tree cultivation and its beekeeping interest. The aim of this research is to develop an Artificial Neural Networks for predict the Castanea pollen concentration in the atmosphere of the North-West Spain area by means a 20years data set. It was detected an increasing trend of the total annual Castanea pollen concentrations in the atmosphere during the study period. The Artificial Neural Networks (ANNs) implemented in this study show a great ability to predict Castanea pollen concentration one, two and three days ahead. The model to predict the Castanea pollen concentration one day ahead shows a high linear correlation coefficient of 0.784 (individual ANN) and 0.738 (multiple ANN). The results obtained improved those obtained by the classical methodology used to predict the airborne pollen concentrations such as time series analysis or other models based on the correlation of pollen levels with meteorological variables. PMID:26802339

  13. Identification of potential sources of airborne Olea pollen in the Southwest Iberian Peninsula.

    PubMed

    Fernández-Rodríguez, Santiago; Skjøth, Carsten Ambelas; Tormo-Molina, Rafael; Brandao, Rui; Caeiro, Elsa; Silva-Palacios, Inmaculada; Gonzalo-Garijo, Angela; Smith, Matt

    2014-04-01

    This study aims to determine the potential origin of Olea pollen recorded in Badajoz in the Southwest of the Iberian Peninsula during 2009-2011. This was achieved using a combination of daily average and diurnal (hourly) airborne Olea pollen counts recorded at Badajoz (south-western Spain) and Évora (south-eastern Portugal), an inventory of olive groves in the studied area and air mass trajectory calculations computed using the HYSPLIT model. Examining olive pollen episodes at Badajoz that had distinctly different diurnal cycles in olive pollen in relation to the mean, allowed us to identify three different scenarios where olive pollen can be transported to the city from either distant or nearby sources during conditions with slow air mass movements. Back trajectory analysis showed that olive pollen can be transported to Badajoz from the West on prevailing winds, either directly or on slow moving air masses, and from high densities of olive groves situated to the Southeast (e.g. Andalucía). Regional scale transport of olive pollen can result in increased nighttime concentrations of this important aeroallergen. This could be particularly important in Mediterranean countries where people can be outdoors during this time due to climate and lifestyle. Such studies that examine sources and the atmospheric transport of pollen are valuable for allergy sufferers and health care professionals because the information can be incorporated into forecasts, the outputs of which are used for avoiding exposure to aeroallergens and planning medication. The results of studies of this nature can also be used for examining gene flow in this important agricultural crop. PMID:23334443

  14. Identification of potential sources of airborne Olea pollen in the Southwest Iberian Peninsula

    NASA Astrophysics Data System (ADS)

    Fernández-Rodríguez, Santiago; Skjøth, Carsten Ambelas; Tormo-Molina, Rafael; Brandao, Rui; Caeiro, Elsa; Silva-Palacios, Inmaculada; Gonzalo-Garijo, Ángela; Smith, Matt

    2014-04-01

    This study aims to determine the potential origin of Olea pollen recorded in Badajoz in the Southwest of the Iberian Peninsula during 2009-2011. This was achieved using a combination of daily average and diurnal (hourly) airborne Olea pollen counts recorded at Badajoz (south-western Spain) and Évora (south-eastern Portugal), an inventory of olive groves in the studied area and air mass trajectory calculations computed using the HYSPLIT model. Examining olive pollen episodes at Badajoz that had distinctly different diurnal cycles in olive pollen in relation to the mean, allowed us to identify three different scenarios where olive pollen can be transported to the city from either distant or nearby sources during conditions with slow air mass movements. Back trajectory analysis showed that olive pollen can be transported to Badajoz from the West on prevailing winds, either directly or on slow moving air masses, and from high densities of olive groves situated to the Southeast (e.g. Andalucía). Regional scale transport of olive pollen can result in increased nighttime concentrations of this important aeroallergen. This could be particularly important in Mediterranean countries where people can be outdoors during this time due to climate and lifestyle. Such studies that examine sources and the atmospheric transport of pollen are valuable for allergy sufferers and health care professionals because the information can be incorporated into forecasts, the outputs of which are used for avoiding exposure to aeroallergens and planning medication. The results of studies of this nature can also be used for examining gene flow in this important agricultural crop.

  15. Modelling airborne concentration and deposition rate of maize pollen

    NASA Astrophysics Data System (ADS)

    Jarosz, Nathalie; Loubet, Benjamin; Huber, Laurent

    2004-10-01

    The introduction of genetically modified (GM) crops has reinforced the need to quantify gene flow from crop to crop. This requires predictive tools which take into account meteorological conditions, canopy structure as well as pollen aerodynamic characteristics. A Lagrangian Stochastic (LS) model, called SMOP-2D (Stochastic Mechanistic model for Pollen dispersion and deposition in 2 Dimensions), is presented. It simulates wind dispersion of pollen by calculating individual pollen trajectories from their emission to their deposition. SMOP-2D was validated using two field experiments where airborne concentration and deposition rate of pollen were measured within and downwind from different sized maize (Zea mays) plots together with micrometeorological measurements. SMOP-2D correctly simulated the shapes of the concentration profiles but generally underestimated the deposition rates in the first 10 m downwind from the source. Potential explanations of this discrepancy are discussed. Incorrect parameterisation of turbulence in the transition from the crop to the surroundings is probably the most likely reason. This demonstrates that LS models for particle transfer need to be coupled with air-flow models under complex terrain conditions.

  16. Airborne pollen and spores of León (Spain)

    NASA Astrophysics Data System (ADS)

    Fernández-González, Delia; Suarez-Cervera, María; Díaz-González, Tomás; Valencia-Barrera, Rosa María

    1993-06-01

    A qualitative and quantitative analysis of airborne pollen and spores was carried out over 2 years (from September 1987 to August 1989) in the city of León. Slides were prepared daily using a volumetric pollen trap, which was placed on the Faculty of Veterinary Science building (University of León) 12m above ground-level. Fifty-one pollen types were observed; the most important of these were: Cupressaceae during the winter, Pinus and Quercus in spring, and Poaceae, Leguminosae and Chenopodiaceae in the summer. The results also showed the existence of a rich mould spore assemblage in the atmosphere. The group of Amerospores ( Penicillium, Aspergillus and Cladosporium) as well as Dictyospores ( Alternaria) were the most abundant; Puccinia was common in the air in August. Fluctuations in the total pollen and spores m3 of air were compared with meteorological parameters (temperature, relative humidity and rainfall). From the daily sampling of the atmosphere of León, considering the maximum and minimum temperature and duration of rainfall, the start of the pollen grain season was observed generally to coincide with a rise in temperature in the absence of rain.

  17. Enhanced airborne radioactivity during a pine pollen release episode.

    PubMed

    Tschiersch, J; Frank, G; Roth, P; Wagenpfeil, F; Watterson, F; Watterson, J

    1999-07-01

    A single episode of pine pollen release in the highly contaminated area of Novozybkov, Russian Federation, which led to enhanced atmospheric concentrations of 137Cs is discussed. The pollen grains were sampled by a rotating arm impactor and analysed by gamma-spectrometry for 137Cs activity and by image analysis for their size. In the vicinity of a forest, a maximum concentration of 4.5+/-0.4 mBq m(-3) was measured, and a mean activity per pollen grain of 260+/-80 nBq was determined. The emission rate of the Novozybkov mixed pine forest was estimated to be approximately 400 Bq m(-2) per year. Because of the large size of pine pollen grains (about 50 microm) and the short emission period of 5-8 days per year, the estimated potential annual inhalation doses are very low. Biological emissions including pollen release may be a source of increased airborne radionuclide concentrations at larger distances from the source areas as well. PMID:10461761

  18. Elemental characterization of the airborne pollen surface using Electron Probe Microanalysis (EPMA)

    NASA Astrophysics Data System (ADS)

    Duque, Laura; Guimarães, Fernanda; Ribeiro, Helena; Sousa, Raquel; Abreu, Ilda

    2013-08-01

    Recent worldwide increase in pollinoses has been attributed to the synergy between pollen and pollutants. We used EPMA for the elemental characterization of the airborne pollen surface in order to find out what occurs to the wall of pollen grains when they are together with other atmospheric pollutants. Analyses were performed both to airborne pollen and to pollen that was collected from Acer spp., Platanus spp. and Pinus spp. trees. Airborne samples were assembled using a Hirst-type volumetric spore sampler set in the coastal city of Porto, Portugal. Airborne pollen samples showed major elemental differences when compared to the control pollen sample of the same species, namely in the amounts of Cl, Na and Mg, which very significantly increased on airborne samples, revealing an important influence of the ocean. Mineral dust also contributed to modify the pollen surface, by increasing Si contents on Acer spp. and Platanus spp. airborne pollen. Our results revealed consistent positive effects of the relative humidity and the precipitation in the increase of Cl, Na and Mg relative amounts on the pollen surface. This study shows that pollen grains have the ability to adsorb and/or absorb other materials, which may contribute to enhance pollen's harmful effects on people's health.

  19. Predicting onset and duration of airborne allergenic pollen season in the United States

    NASA Astrophysics Data System (ADS)

    Zhang, Yong; Bielory, Leonard; Cai, Ting; Mi, Zhongyuan; Georgopoulos, Panos

    2015-02-01

    Allergenic pollen is one of the main triggers of Allergic Airway Disease (AAD) affecting 5%-30% of the population in industrialized countries. A modeling framework has been developed using correlation and collinearity analyses, simulated annealing, and stepwise regression based on nationwide observations of airborne pollen counts and climatic factors to predict the onsets and durations of allergenic pollen seasons of representative trees, weeds and grass in the contiguous United States. Main factors considered are monthly, seasonal and annual mean temperatures and accumulative precipitations, latitude, elevation, Growing Degree Day (GDD), Frost Free Day (FFD), Start Date (SD) and Season Length (SL) in the previous year. The estimated mean SD and SL for birch (Betula), oak (Quercus), ragweed (Ambrosia), mugwort (Artemisia) and grass (Poaceae) pollen season in 1994-2010 are mostly within 0-6 days of the corresponding observations for the majority of the National Allergy Bureau (NAB) monitoring stations across the contiguous US. The simulated spatially resolved maps for onset and duration of allergenic pollen season in the contiguous US are consistent with the long term observations.

  20. Predicting Onset and Duration of Airborne Allergenic Pollen Season in the United States

    PubMed Central

    Zhang, Yong; Bielory, Leonard; Cai, Ting; Mi, Zhongyuan; Georgopoulos, Panos

    2014-01-01

    Allergenic pollen is one of the main triggers of Allergic Airway Disease (AAD) affecting 5% to 30% of the population in industrialized countries. A modeling framework has been developed using correlation and collinearity analyses, simulated annealing, and stepwise regression based on nationwide observations of airborne pollen counts and climatic factors to predict the onsets and durations of allergenic pollen seasons of representative trees, weeds and grass in the contiguous United States. Main factors considered are monthly, seasonal and annual mean temperatures and accumulative precipitations, latitude, elevation, Growing Degree Day (GDD), Frost Free Day (FFD), Start Date (SD) and Season Length (SL) in the previous year. The estimated mean SD and SL for birch (Betula), oak (Quercus), ragweed (Ambrosia), mugwort (Artemisia) and grass (Poaceae) pollen season in 1994–2010 are mostly within 0 to 6 days of the corresponding observations for the majority of the National Allergy Bureau (NAB) monitoring stations across the contiguous US. The simulated spatially resolved maps for onset and duration of allergenic pollen season in the contiguous US are consistent with the long term observations. PMID:25620875

  1. Variations of airborne winter pollen in southern Spain.

    PubMed

    Ruiz de Clavijo, E; Galán, C; Infante, F; Domínguez, E

    1988-01-01

    This work deals with the variation in the atmosphere of the airborne pollen produced by winter blooming plants and is aimed to establish correlations between the concentration of pollen grains in the atmosphere of Córdoba and meteorological parameters such as the temperature, humidity, rainfall, pressure, hours of sunlight and wind speed and direction. The work was conducted for two consecutive years (1981-82 and 1982-83). The sampling was carried out in Córdoba (Spain) with a BURKARD sporetrap. The data obtained in the aeropalinological study are correlated to the above-mentioned meterological parameters. Along the period investigated grains were found from Ulmus minor, Fraxinus sp., Populus sp., Alnus glutinosa and Cupressaceae, and less frequently, Artemisia sp., Pinaceae, Urticaceae, type Helianthus and Gramineae. Pollen grains from Cupressaceae were found at the highest absolute and relative concentrations in the atmosphere of Córdoba during the winter, where they occurred almost throughout. The correlation analysis applied showed that the parameters most markedly influencing the grain concentration of most taxa were the temperature and humidity. Alnus glutinosa was the least affected species, probably because of the scarcity of its pollen grains. PMID:3177155

  2. The occurrence and allergising potential of airborne pollen in West Bengal, India.

    PubMed

    Boral, Dola; Chatterjee, Soma; Bhattacharya, Kashinath

    2004-01-01

    A continuous 2-year volumetric aerobiological survey was conducted in Berhampore town, a centrally located and representative part of West Bengal, India. The aim of the study was to assess the allergising potential of airborne pollen grains of West Bengal. A total of 31 pollen types were identified of which Poaceae (grasses) pollen showed maximum frequency, followed by Cyperaceae, Cassia sp., Acacia auriculiformis, etc. The seasonal periodicities of the pollen types and their relationship to meteorological conditions were investigated. It was found that the pollen concentration is positively correlated with temperature and negatively correlated with rainfall and relative humidity. Clinical investigations by skin prick test were carried out to detect allergenicity of pollen types. Eighteen common airborne pollen types induced positive responses of which pollen extracts of Saccharum officinarum (grass), Azadirachta indica, Cocos nucifera, Phoenix sylvestris, Cyperus rotundus and Eucalyptus citriodora showed strongest sensitising potential. This result is consistent with previous investigations in different parts of West Bengal. PMID:15236497

  3. Can we improve pollen season definitions by using the symptom load index in addition to pollen counts?

    PubMed

    Bastl, Katharina; Kmenta, Maximilian; Geller-Bernstein, Carmi; Berger, Uwe; Jäger, Siegfried

    2015-09-01

    Airborne pollen measurements are the foundation of aerobiological research and provide essential raw data for various disciplines. Pollen itself should be considered a relevant factor in air quality. Symptom data shed light on the relationship of pollen allergy and pollination. The aim of this study is to assess the spatial variation of local, regional and national symptom datasets. Ten pollen season definitions are used to calculate the symptom load index for the birch and grass pollen seasons (2013-2014) in Austria. (1) Local, (2) regional and (3) national symptom datasets are used to examine spatial variations and a consistent pattern was found. In conclusion, national datasets are suitable for first insights where no sufficient local or regional dataset is available and season definitions based on percentages provide a practical solution, as they can be applied in regions with different pollen loads and produce more constant results. PMID:25935611

  4. Bayesian analysis of climate change effects on observed and projected airborne levels of birch pollen

    NASA Astrophysics Data System (ADS)

    Zhang, Yong; Isukapalli, Sastry S.; Bielory, Leonard; Georgopoulos, Panos G.

    2013-04-01

    A Bayesian framework is presented for modeling effects of climate change on pollen indices such as annual birch pollen count, maximum daily birch pollen count, start date of birch pollen season and the date of maximum daily birch pollen count. Annual mean CO2 concentration, mean spring temperature and the corresponding pollen index of prior year were found to be statistically significant accounting for effects of climate change on four pollen indices. Results suggest that annual productions and peak values from 2020 to 2100 under different scenarios will be 1.3-8.0 and 1.1-7.3 times higher respectively than the mean values for 2000, and start and peak dates will occur around two to four weeks earlier. These results have been partly confirmed by the available historical data. As a demonstration, the emission profiles in future years were generated by incorporating the predicted pollen indices into an existing emission model.

  5. Bayesian Analysis of Climate Change Effects on Observed and Projected Airborne Levels of Birch Pollen.

    PubMed

    Zhang, Yong; Isukapalli, Sastry; Bielory, Leonard; Georgopoulos, Panos

    2013-04-01

    A Bayesian framework is presented for modeling Effects of climate change on pollen indices such as annual birch pollen count, maximum daily birch pollen count, start date of birch pollen season and the date of maximum daily birch pollen count. Annual mean CO2 concentration, mean spring temperature and the corresponding pollen index of prior year were found to be statistically significant accounting for Effects of climate change on four pollen indices. Results suggest that annual productions and peak values from 2020 to 2100 under different scenarios will be 1.3-8.0 and 1.1-7.3 times higher respectively than the mean values for 2000, and start and peak dates will occur around two to four weeks earlier. These results have been partly confirmed by the available historical data. As a demonstration, the emission profiles in future years were generated by incorporating the predicted pollen indices into an existing emission model. PMID:23526049

  6. Bayesian Analysis of Climate Change Effects on Observed and Projected Airborne Levels of Birch Pollen

    PubMed Central

    Zhang, Yong; Isukapalli, Sastry; Bielory, Leonard; Georgopoulos, Panos

    2012-01-01

    A Bayesian framework is presented for modeling Effects of climate change on pollen indices such as annual birch pollen count, maximum daily birch pollen count, start date of birch pollen season and the date of maximum daily birch pollen count. Annual mean CO2 concentration, mean spring temperature and the corresponding pollen index of prior year were found to be statistically significant accounting for Effects of climate change on four pollen indices. Results suggest that annual productions and peak values from 2020 to 2100 under different scenarios will be 1.3-8.0 and 1.1-7.3 times higher respectively than the mean values for 2000, and start and peak dates will occur around two to four weeks earlier. These results have been partly confirmed by the available historical data. As a demonstration, the emission profiles in future years were generated by incorporating the predicted pollen indices into an existing emission model. PMID:23526049

  7. Effects of climate change and seed dispersal on airborne ragweed pollen loads in Europe

    NASA Astrophysics Data System (ADS)

    Hamaoui-Laguel, Lynda; Vautard, Robert; Liu, Li; Solmon, Fabien; Viovy, Nicolas; Khvorostyanov, Dmitry; Essl, Franz; Chuine, Isabelle; Colette, Augustin; Semenov, Mikhail A.; Schaffhauser, Alice; Storkey, Jonathan; Thibaudon, Michel; Epstein, Michelle M.

    2015-08-01

    Common ragweed (Ambrosia artemisiifolia) is an invasive alien species in Europe producing pollen that causes severe allergic disease in susceptible individuals. Ragweed plants could further invade European land with climate and land-use changes. However, airborne pollen evolution depends not only on plant invasion, but also on pollen production, release and atmospheric dispersion changes. To predict the effect of climate and land-use changes on airborne pollen concentrations, we used two comprehensive modelling frameworks accounting for all these factors under high-end and moderate climate and land-use change scenarios. We estimate that by 2050 airborne ragweed pollen concentrations will be about 4 times higher than they are now, with a range of uncertainty from 2 to 12 largely depending on the seed dispersal rate assumptions. About a third of the airborne pollen increase is due to on-going seed dispersal, irrespective of climate change. The remaining two-thirds are related to climate and land-use changes that will extend ragweed habitat suitability in northern and eastern Europe and increase pollen production in established ragweed areas owing to increasing CO2. Therefore, climate change and ragweed seed dispersal in current and future suitable areas will increase airborne pollen concentrations, which may consequently heighten the incidence and prevalence of ragweed allergy.

  8. Modular method of detection, localization, and counting of multiple-taxon pollen apertures using bag-of-words

    NASA Astrophysics Data System (ADS)

    Lozano-Vega, Gildardo; Benezeth, Yannick; Marzani, Franck; Boochs, Frank

    2014-09-01

    Accurate recognition of airborne pollen taxa is crucial for understanding and treating allergic diseases which affect an important proportion of the world population. Modern computer vision techniques enable the detection of discriminant characteristics. Apertures are among the important characteristics which have not been adequately explored until now. A flexible method of detection, localization, and counting of apertures of different pollen taxa with varying appearances is proposed. Aperture description is based on primitive images following the bag-of-words strategy. A confidence map is estimated based on the classification of sampled regions. The method is designed to be extended modularly to new aperture types employing the same algorithm by building individual classifiers. The method was evaluated on the top five allergenic pollen taxa in Germany, and its robustness to unseen particles was verified.

  9. Fifteen years' record of airborne allergenic pollen and meteorological parameters in Thessaloniki, Greece

    NASA Astrophysics Data System (ADS)

    Gioulekas, Dimitrios; Balafoutis, Christos; Damialis, Athanasios; Papakosta, Despoina; Gioulekas, George; Patakas, Dimitrios

    . A pollen calendar has been constructed for the area of Thessaloniki and relationships between pollen transport and meteorological parameters have been assessed. Daily airborne pollen records were collected over a 15-year period (1987-2001), using a Burkard continuous volumetric pollen trap, located in the centre of the city. Sixteen allergenic pollen types were identified. Simultaneously, daily records of five main meteorological parameters (mean air temperature, relative humidity, rainfall, sunshine, wind speed) were made, and then correlated with fluctuations of the airborne pollen concentrations. For the first time in Greece, a pollen calendar has been constructed for 16 pollen types, from which it appears that 24.9% of the total pollen recorded belong to Cupressaceae, 20.8% to Quercus spp., 13.6% to Urticaceae, 9.1% to Oleaceae, 8.9% to Pinaceae, 6.3% to Poaceae, 5.4% to Platanaceae, 3.0% to Corylus spp., 2.5% to Chenopodiaceae and 1.4% to Populus spp. The percentages of Betula spp., Asteraceae (Artemisia spp. and Ambrosia spp.), Salix spp., Ulmaceae and Alnus spp. were each lower than 1%. A positive correlation between pollen transport and both mean temperature and sunshine was observed, whereas usually no correlation was found between pollen and relative humidity or rainfall. Finally, wind speed was generally found to have a significant positive correlation with the concentrations of 8 pollen types. For the first time in the area of Thessaloniki, and more generally in Greece, 15-year allergenic pollen records have been collected and meteorological parameters have been recorded. The airborne pollen concentration is strongly influenced by mean air temperature and sunshine duration. The highest concentrations of pollen grains are observed during spring (May).

  10. Seasonal appearance of grass pollen allergen in natural, pauci-micronic aerosol of various size fractions. Relationship with airborne grass pollen concentration.

    PubMed

    Spieksma, F T; Nikkels, B H; Dijkman, J H

    1995-03-01

    In a study during the 1993 grass pollen season at Leiden, the relationship between atmospheric pollen allergen carried by five size fractions of pauci-micronic (few microns) particles and the grass pollen count was investigated. Sampling was carried out on dry days, and atmospheric pollen allergen in the particle fractions was assessed by a RAST-inhibition assay while grass pollen quantities were measured with a volumetric pollen trap. It appears that the atmospheric presence of grass pollen allergen in all size fractions is restricted mainly to the period of presence of grass pollen grains. Before and after the grass pollen season atmospheric grass pollen allergen quantities are generally very low. It is concluded that a routinely performed grass pollen count is a reliable measurement for the estimation of the amount of atmospheric grass pollen allergen, also in the pauci-micronic particle fraction. PMID:7788570

  11. [Seasonal Dynamics of Airborne Pollens and Its Relationship with Meteorological Factors in Beijing Urban Area].

    PubMed

    Meng, Ling; Wang, Xiao-ke; Ouyang, Zhi-yun; Ren, Yu-fen; Wang, Qiao-huan

    2016-02-15

    The seasonal dynamics of airborne pollens and their relationship with meteorological conditions, which are considered to be important factors for appropriate construction of urban green system and reliable prevention of tropic pollinosis, were investigated in Beijing urban area. The airborne pollens were monitored from December 31st 2011 to December 31st 2012 by Burkard volumetric trap, and the data were analyzed. The results revealed that: (1) In 2012 the pollen dispersion period lasted 238 days from March 17 to November 18th, accounting for 65% of the year. There were two peaks of pollen amount in air, which occurred from March to May and from August to October, respectively. In the spring peak, tree pollens such as Oleaceae, Populus and Salix pollens were the dominant, accounting for 53% of the total annual pollens, while in the autumn period, weed pollens such as Compositae, Chenopodiaceae and Amaranthaceae pollens made up about 40% of the annual total value; (2) The highly allergenic weeds pollens dominated in autumn, which caused a high incidence of tropic pollinosis; (3) The airborne pollen amount of Beijing urban area was significantly affected by meteorological condition like the wind speed, temperature, humidity, precipitation and so on; (4) When temperature ranged from OC to 15 degrees C, the pollen amount showed positive relation with temperature; while in the temperature range of 18 degrees C to 30 degrees C, it showed negative relation; (5) The average temperature of spring and autumn season in 2012 was 17 degrees C, and 79% of airborne pollens were detected in these two seasons. This temperature condition was conducive to the pollen dispersion. (6) The pollen amount showed negative relation with relative moisture between 20% and 50% and larger than 70%, while in the moisture range of 50% to 60%, it showed positive relation; (7) The wind speed smaller than 3 m x s(-1) was good to pollen distribution, when it was larger than 4 m x s(-1) or the wind

  12. Diurnal variation in airborne pollen concentrations of the selected taxa in Zagreb, Croatia.

    PubMed

    Toth, Ivan; Peternel, Renata; Srnec, Lidija; Vojniković, Bozo

    2011-09-01

    The number of individuals allergic to plant pollen has recently been on a constant increase. The knowledge of diurnal distribution and abundance of allergenic pollen types, their patterns and response to source position and weather is useful to correlate hay fever symptoms with the presence of allergenic pollen in the atmosphere. The aim of this study was to determine diurnal distribution of total airborne pollen, pollen of particular allergenic taxa, possible variation in diurnal pollen distribution at measuring sites placed at different heights, and effect of some meteorological parameters on airborne pollen concentrations. A 7-day Hirst-type volumetric pollen trap was used for pollen sampling. Qualitative and quantitative pollen analysis was performed under a light microscope (magnification x400). Total pollen of all plant taxa (Ambrosia sp., Betula sp., Cupressaceae, Urticaceae, Poaceae, Quercus sp., Fraxinus sp., Alnus sp., Corylus sp., Populus sp., Pinus sp., Picea sp.) observed showed a regular diurnal distribution at both sampling sites in both study years, with a rise in the pollen concentration recorded after 4.00 a.m. and 6.00 a.m., respectively. The peak pollen concentration occurred between 12.00 a.m. and 4.00 p.m., and the lowest diurnal pollen concentrations were recorded overnight. About 50% of the 24-h pollen concentration were released to the atmosphere between 10.00 a.m. and 4.00 p.m. The timing and size of diurnal peaks were closely related to high temperature, low humidity and south-west maximum wind direction. PMID:22220402

  13. Association between first airborne cedar pollen level peak and pollinosis symptom onset: a web-based survey.

    PubMed

    Bando, Harumi; Sugiura, Hiroaki; Ohkusa, Yasushi; Akahane, Manabu; Sano, Tomomi; Jojima, Noriko; Okabe, Nobuhiko; Imamura, Tomoaki

    2015-01-01

    Cedar pollinosis in Japan affects nearly 25 % of Japanese citizens. To develop a treatment for cedar pollinosis, it is necessary to understand the relationship between the time of its occurrence and the amount of airborne cedar pollen. In the spring of 2009, we conducted daily Internet-based epidemiologic surveys, which included 1453 individuals. We examined the relationship between initial date of onset of pollinosis symptoms and daily amount of airborne cedar pollen to which subjects were exposed. Approximately 35.2 % of the subjects experienced the onset of pollinosis during a one-week interval in which the middle day coincided with the peak pollen count. The odds ratio for this one-week time interval was 4.03 (95 % confidence interval: 3.34-4.86). The predicted date of the cedar pollen peak can be used to determine the appropriate date for initiation of self-medication with anti-allergy drugs and thus avoid development of sustained and severe pollinosis. PMID:24720339

  14. Trends and threshold exceedances analysis of airborne pollen concentrations in Metropolitan Santiago Chile

    PubMed Central

    Toro A., Richard; Córdova J., Alicia; Canales, Mauricio; Morales S., Raul G. E.; Mardones P., Pedro; Leiva G., Manuel A.

    2015-01-01

    Pollen is one of the primary causes of allergic rhinoconjunctivitis in urban centers. In the present study, the concentrations of 39 different pollens in the Santiago de Chile metropolitan area over the period 2009–2013 are characterized. The pollen was monitored daily using Burkard volumetric equipment. The contribution of each type of pollen and the corresponding time trends are evaluated. The concentrations of the pollens are compared with the established threshold levels for the protection of human health. The results show that the total amount of pollen grains originating from trees, grasses, weeds and indeterminate sources throughout the period of the study was 258,496 grains m-3, with an annual average of 51,699 ± 3,906 grains m-3 year-1. The primary source of pollen is Platanus orientalis, which produces 61.8% of the analyzed pollen. Grass pollen is the third primary component of the analyzed pollen, with a contribution of 5.82%. Among the weeds, the presence of Urticacea (3.74%) is remarkable. The pollination pattern of the trees is monophasic, and the grasses have a biphasic pattern. The trends indicate that the total pollen and tree pollen do not present a time trend that is statistically significant throughout the period of the study, whereas the grass pollen and weed pollen concentrations in the environment present a statistically significant decreasing trend. The cause of this decrease is unclear. The pollen load has doubled over the past decade. When the observed concentrations of the pollens were compared with the corresponding threshold levels, the results indicated that over the period of the study, the pollen concentrations were at moderate, high and very high levels for an average of 293 days per year. Systematic counts of the pollen grains are an essential method for diagnosing and treating patients with pollinosis and for developing forestation and urban planning strategies. PMID:25946339

  15. Trends and threshold exceedances analysis of airborne pollen concentrations in Metropolitan Santiago Chile.

    PubMed

    Toro A, Richard; Córdova J, Alicia; Canales, Mauricio; Morales S, Raul G E; Mardones P, Pedro; Leiva G, Manuel A

    2015-01-01

    Pollen is one of the primary causes of allergic rhinoconjunctivitis in urban centers. In the present study, the concentrations of 39 different pollens in the Santiago de Chile metropolitan area over the period 2009-2013 are characterized. The pollen was monitored daily using Burkard volumetric equipment. The contribution of each type of pollen and the corresponding time trends are evaluated. The concentrations of the pollens are compared with the established threshold levels for the protection of human health. The results show that the total amount of pollen grains originating from trees, grasses, weeds and indeterminate sources throughout the period of the study was 258,496 grains m-3, with an annual average of 51,699 ± 3,906 grains m-3 year-1. The primary source of pollen is Platanus orientalis, which produces 61.8% of the analyzed pollen. Grass pollen is the third primary component of the analyzed pollen, with a contribution of 5.82%. Among the weeds, the presence of Urticacea (3.74%) is remarkable. The pollination pattern of the trees is monophasic, and the grasses have a biphasic pattern. The trends indicate that the total pollen and tree pollen do not present a time trend that is statistically significant throughout the period of the study, whereas the grass pollen and weed pollen concentrations in the environment present a statistically significant decreasing trend. The cause of this decrease is unclear. The pollen load has doubled over the past decade. When the observed concentrations of the pollens were compared with the corresponding threshold levels, the results indicated that over the period of the study, the pollen concentrations were at moderate, high and very high levels for an average of 293 days per year. Systematic counts of the pollen grains are an essential method for diagnosing and treating patients with pollinosis and for developing forestation and urban planning strategies. PMID:25946339

  16. Environmental Factors Affecting Asthma and Allergies: Predicting and Simulating Downwind Exposure to Airborne Pollen

    NASA Technical Reports Server (NTRS)

    Luvall, Jeffrey; Estes, Sue; Sprigg, William A.; Nickovic, Slobodan; Huete, Alfredo; Solano, Ramon; Ratana, Piyachat; Jiang, Zhangyan; Flowers, Len; Zelicoff, Alan

    2009-01-01

    This slide presentation reviews the environmental factors that affect asthma and allergies and work to predict and simulate the downwind exposure to airborne pollen. Using a modification of Dust REgional Atmosphere Model (DREAM) that incorporates phenology (i.e. PREAM) the aim was to predict concentrations of pollen in time and space. The strategy for using the model to simulate downwind pollen dispersal, and evaluate the results. Using MODerate-resolution Imaging Spectroradiometer (MODIS), to get seasonal sampling of Juniper, the pollen chosen for the study, land cover on a near daily basis. The results of the model are reviewed.

  17. Time series predictions with neural nets: Application to airborne pollen forecasting

    NASA Astrophysics Data System (ADS)

    Arizmendi, C. M.; Sanchez, J. R.; Ramos, N. E.; Ramos, G. I.

    1993-09-01

    Pollen allergy is a common disease causing rhinoconjunctivitis (hay fever) in 5 10% of the population. Medical studies have indicated that pollen related diseases could be highly reduced if future pollen contents in the air could be predicted. In this work we have developed a new forecasting method that applies the ability of neural nets to predict the future behaviour of chaotic systems in order to make accurate predictions of the airborne pollen concentration. The method requires that the neural net be fed with non-zero values, which restricts the method predictions to the period following the start of pollen flight. The operational method outlined here constitutes a different point of view with respect to the more generally used forecasts of time series analysis, which require input of many meteorological parameters. Excellent forecasts were obtained training a neural net by using only the time series pollen concentration values.

  18. Temporal modelling and forecasting of the airborne pollen of Cupressaceae on the southwestern Iberian Peninsula

    NASA Astrophysics Data System (ADS)

    Silva-Palacios, Inmaculada; Fernández-Rodríguez, Santiago; Durán-Barroso, Pablo; Tormo-Molina, Rafael; Maya-Manzano, José María; Gonzalo-Garijo, Ángela

    2016-02-01

    Cupressaceae includes species cultivated as ornamentals in the urban environment. This study aims to investigate airborne pollen data for Cupressaceae on the southwestern Iberian Peninsula over a 21-year period and to analyse the trends in these data and their relationship with meteorological parameters using time series analysis. Aerobiological sampling was conducted from 1993 to 2013 in Badajoz (SW Spain). The main pollen season for Cupressaceae lasted, on average, 58 days, ranging from 55 to 112 days, from 24 January to 22 March. Furthermore, a short-term forecasting model has been developed for daily pollen concentrations. The model proposed to forecast the airborne pollen concentration is described by one equation. This expression is composed of two terms: the first term represents the pollen concentration trend in the air according to the average concentration of the previous 10 days; the second term is obtained from considering the actual pollen concentration value, which is calculated based on the most representative meteorological parameters multiplied by a fitting coefficient. Temperature was the main meteorological factor by its influence over daily pollen forecast, being the rain the second most important factor. This model represents a good approach to a continuous balance model of Cupressaceae pollen concentration and is supported by a close agreement between the observed and predicted mean concentrations. The novelty of the proposed model is the analysis of meteorological parameters that are not frequently used in Aerobiology.

  19. Temporal modelling and forecasting of the airborne pollen of Cupressaceae on the southwestern Iberian Peninsula.

    PubMed

    Silva-Palacios, Inmaculada; Fernández-Rodríguez, Santiago; Durán-Barroso, Pablo; Tormo-Molina, Rafael; Maya-Manzano, José María; Gonzalo-Garijo, Ángela

    2016-02-01

    Cupressaceae includes species cultivated as ornamentals in the urban environment. This study aims to investigate airborne pollen data for Cupressaceae on the southwestern Iberian Peninsula over a 21-year period and to analyse the trends in these data and their relationship with meteorological parameters using time series analysis. Aerobiological sampling was conducted from 1993 to 2013 in Badajoz (SW Spain). The main pollen season for Cupressaceae lasted, on average, 58 days, ranging from 55 to 112 days, from 24 January to 22 March. Furthermore, a short-term forecasting model has been developed for daily pollen concentrations. The model proposed to forecast the airborne pollen concentration is described by one equation. This expression is composed of two terms: the first term represents the pollen concentration trend in the air according to the average concentration of the previous 10 days; the second term is obtained from considering the actual pollen concentration value, which is calculated based on the most representative meteorological parameters multiplied by a fitting coefficient. Temperature was the main meteorological factor by its influence over daily pollen forecast, being the rain the second most important factor. This model represents a good approach to a continuous balance model of Cupressaceae pollen concentration and is supported by a close agreement between the observed and predicted mean concentrations. The novelty of the proposed model is the analysis of meteorological parameters that are not frequently used in Aerobiology. PMID:26092133

  20. Allergenic airborne pollen and spores in Anchorage, Alaska

    SciTech Connect

    Anderson, J.H.

    1985-05-01

    Major aeroallergens in Anchorage are birch, alder, poplar, spruce, grass pollen, Cladosporium, and unspecified fungus spores. Lesser pollens are sorrel, willow, pine, juniper, sedge, lamb's-quarters, wormwood, plantain, and others. The aero-flora is discussed in terms of the frequency of allergenically significant events and within-season and year-to-year dynamics.

  1. Airborne birch and grass pollen allergens in street-level shops.

    PubMed

    Holmquist, L; Weiner, J; Vesterberg, O

    2001-12-01

    Polluted urban outdoor air may be enriched with large amounts of submicronic respirable pollen allergen particles that penetrate into street-level shops. The objectives of the study were to map concentrations of birch and grass pollen allergens in indoor air of street-level shops and to explore the effect of electrostatic air cleaning under authentic working conditions, indoor air samples were collected in May and July 1999 in two shops. Allergens were quantified by a direct on sampling filter in solution (DOSIS) luminescence immunoassay. The average concentration of airborne indoor birch pollen allergen in the shop with air cleaning was estimated to be 20 +/- 9 SQ/m3 (mean +/- SD) compared to 31 +/- 17 SQ/m3 (mean +/- SD) of that without. The air cleaner reduced the indoor air birch pollen allergen concentration by on average 26 to 48% (P < 0.05). Corresponding figures for airborne indoor grass pollen allergen concentrations were 14 +/- 7 SQ/m3 and 17 +/- 8 SQ/m3, indicating a statistically non-significant (t-test) average 18% reduction of allergen by air cleaning. Excluding two observations with poor fit to the statistical model a significant (P < 0.05) average 27% reduction was obtained. Substantial amounts of airborne birch and grass pollen allergens may occur in street-level shops during flowering seasons. PMID:11761599

  2. The Macroecology of Airborne Pollen in Australian and New Zealand Urban Areas

    PubMed Central

    Haberle, Simon G.; Bowman, David M. J. S.; Newnham, Rewi M.; Johnston, Fay H.; Beggs, Paul J.; Buters, Jeroen; Campbell, Bradley; Erbas, Bircan; Godwin, Ian; Green, Brett J.; Huete, Alfredo; Jaggard, Alison K.; Medek, Danielle; Murray, Frank; Newbigin, Ed; Thibaudon, Michel; Vicendese, Don; Williamson, Grant J.; Davies, Janet M.

    2014-01-01

    The composition and relative abundance of airborne pollen in urban areas of Australia and New Zealand are strongly influenced by geographical location, climate and land use. There is mounting evidence that the diversity and quality of airborne pollen is substantially modified by climate change and land-use yet there are insufficient data to project the future nature of these changes. Our study highlights the need for long-term aerobiological monitoring in Australian and New Zealand urban areas in a systematic, standardised, and sustained way, and provides a framework for targeting the most clinically significant taxa in terms of abundance, allergenic effects and public health burden. PMID:24874807

  3. Controlling Hay Fever Symptoms with Accurate Pollen Counts

    MedlinePlus

    ... Dictionary Just for Kids Library School Tools Videos Virtual Allergist Education & Training Careers in A/I Continuing ... period. The instrument is then taken to a lab where the collected material is analyzed for pollen ...

  4. Seasonal and intradiurnal variation of airborne pollen concentrations in Bodrum, SW Turkey.

    PubMed

    Tosunoglu, Aycan; Bicakci, Adem

    2015-04-01

    An aeropalynological study was performed in Bodrum, the famous tourism center in southwestern Turkey with a Hirst-type volumetric 7-day pollen and spore trap for 2 years (2007-2008). In Bodrum, 25,099 pollen grains as a mean value belonging to 41 taxa were recorded annually during the study period, and pollen grains from woody plant taxa had the largest atmospheric contribution of 86.99% and 24 taxa. However, 17 herbaceous plant taxa constituted 12.82% of the annual total pollen count, and 0.19% were unidentified. An average annual pollen index of 22.66% was recorded in March, despite differences from year to year. The highest pollen variability of 34 taxa was recorded in April and May. Predominant pollen types belonged to Cupressaceae/Taxaceae (42.73%), Quercus (15.95%), Pinus (9.78%), Olea europaea (9.04%), Poaceae (5.50%), Betula (1.82%), Pistacia (1.74%), Morus (1.72%), Urticaceae (1.46%), and Plantago (1.28%) and generated 91.03 of the annual total. In total, 32.59% of the mean annual total pollen index was recorded in the morning, and less pollen was recorded in the evening (18.71%). Maximum pollen concentration was recorded between 11:00 and 12:00 a.m. PMID:25750068

  5. Variations in mugwort (Artemisia spp.) airborne pollen concentrations at three sites in central Croatia, in period from 2002 to 2003.

    PubMed

    Peternel, Renata; Hrga, Ivana; Culig, Josip

    2006-12-01

    In spite of the low atmospheric pollen levels, Artemisia sensitisation and allergy has been reported widely. The aim of the study was to determine the length of pollen season, intradiurnal, daily and monthly pollen variation, and the effect of some meteorological parameters on atmospheric pollen concentrations in Central Croatia. Seven-day Hirst volumetric pollen and spore traps were used for pollen sampling. The Artemisia pollen season lasted from the end of July until the end of September with the highest concentrations in August. The percentage of the total pollen count ranged from 0.52% to 0.92%. The intradiurnal peak occurred between 10 a.m. and 12 a.m. Statistical analysis showed a significant correlations between higher air temperature and high pollen concentration as well as high precipitation and low pollen concentration. Results of this study are expected to help in preventing the symptoms of allergic reaction in individuals with Artemisia pollen hypersensitivity. PMID:17243566

  6. Detection of airborne genetically modified maize pollen by real-time PCR.

    PubMed

    Folloni, Silvia; Kagkli, Dafni-Maria; Rajcevic, Bojan; Guimarães, Nilson C C; Van Droogenbroeck, Bart; Valicente, Fernando H; Van den Eede, Guy; Van den Bulcke, Marc

    2012-09-01

    The cultivation of genetically modified (GM) crops has raised numerous concerns in the European Union and other parts of the world about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dispersal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GM maize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semi-urban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GM pollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this 'GM pollen monitoring by bioaerosol sampling and PCR screening' approach might represent an useful aid in the surveillance of GM-free areas, centres of origin and natural reserves. PMID:22805239

  7. Pollen count and exhaled nitric oxide levels in a seasonal allergic rhinitis patient.

    PubMed

    Shirai, Toshihiro; Mochizuki, Eisuke; Asada, Kazuhiro; Suda, Takafumi

    2014-09-01

    The subject was a 52-year-old man with Japanese cedar pollinosis, which developed between February and May. He had no history of asthma and was an ex-smoker. He underwent fractional exhaled nitric oxide (FeNO) measurements twice a week from 2010 to 2012. The pollen counts in 2010 were the lowest during the last decade, and the FeNO level was less than 30 ppb for the whole year. In contrast, the mean pollen count in 2011 was very high and the patient's FeNO level rose to more than 100 ppb. The mean pollen count in 2012 was comparable with that of 2010; however, high counts were detected in April and May, and the FeNO level rose to 70 ppb during the latter stages of the pollen season. These results indicate that pollen counts should be taken into consideration during the interpretation of FeNO data in asthma or allergic rhinitis. PMID:25473586

  8. Immunologic, spectrophotometric and nucleic acid based methods for the detection and quantification of airborne pollen

    PubMed Central

    Rittenour, William R.; Hamilton, Robert G.; Beezhold, Donald H.; Green, Brett J.

    2015-01-01

    Microscopic identification of pollen morphological phenotypes has been the traditional method used to identify and quantify pollen collected by air monitoring stations worldwide. Although this method has enabled a semi-standardized approach for the assessment of pollen exposure, limitations including labor intensiveness, required expertise, examiner bias, and the inability to differentiate species, genera, and in some cases families have limited data derived from the these stations. Recent advances in chemical, biochemical and molecular detection methods have provided standardized alternatives to this microscopic approach. In this review, we examine the applicability of alternative methodologies, in particular nucleic acid based assays involving the quantitative polymerase chain reaction, for the standardized detection of airborne pollen. PMID:22342607

  9. Trends in prevalence of allergic rhinitis and correlation with pollen counts in Switzerland

    NASA Astrophysics Data System (ADS)

    Frei, Thomas; Gassner, Ewald

    2008-11-01

    In recent decades, a large number of epidemiological studies investigating the change of prevalence of hay fever showed an increase in the occurrence of this disease. However, other studies carried out in the 1990s yielded contradictory results. Many environmental factors have been hypothesized to contribute to the increasing hay fever rate, including both indoor and ambient air pollution, reduced exposure to microbial stimulation and changes in diets. However, the observed increase has not convincingly been explained by any of these factors and there is limited evidence of changes in exposure to these risk factors over time. Additionally, recent studies show that no further increase in asthma, hay fever and atopic sensitisation in adolescents and adults has been observed during the 1990s and the beginning of the new century. As the pattern of pollen counts has changed over the years, partly due to the global warming but also as a consequence of a change in the use of land, the changing prevalence of hay fever might partly be driven by this different pollen exposure. Epidemiological data for hay fever in Switzerland are available from 1926 until 2000 (with large gaps between 1926 and 1958 and 1958 to 1986) whereas pollen data are available from 1969 until the present. This allows an investigation as to whether these data are correlated provided the same time spans are compared. It would also be feasible to correlate the pollen data with meteorological data which, however, is not the subject of our investigation. Our study focuses on analyzing time series of pollen counts and of pollen season lengths in order to identify their trends, and to ascertain whether there is a relationship between these trends and the changes in the hay fever prevalence. It is shown in this paper that the pollen exposure has been decreasing in Basel since the beginning of the 1990s whereas the rate of the hay fever prevalence in Switzerland remained approximately unchanged in this period

  10. Pollen Count and Presentation of Angiotensin-Converting Enzyme Inhibitor-Associated Angioedema

    PubMed Central

    Straka, Brittany; Nian, Hui; Sloan, Chantel; Byrd, James Brian; Woodard-Grice, Alencia; Yu, Chang; Stone, Elizabeth; Steven, Gary; Hartert, Tina; Teo, Koon K.; Pare, Guillaume; McCarty, Catherine A.; Brown, Nancy J.

    2014-01-01

    BACKGROUND The incidence of angiotensin-converting enzyme (ACE) inhibitor-associated angioedema is increased in patients with seasonal allergies. OBJECTIVE We tested the hypothesis that patients with ACE inhibitor-associated angioedema present during months when pollen counts are increased. METHODS Cohort analysis examined the month of presentation of ACE inhibitor-associated angioedema and pollen counts in the ambulatory and hospital setting. Patients with ACE inhibitor-associated angioedema were ascertained through (1) an observational study of patients presenting to Vanderbilt University Medical Center, (2) patients presenting to the Marshfield Clinic and participating in the Marshfield Clinic Personalized Medicine Research Project, and (3) patients enrolled in The Ongoing Telmisartan Alone and in Combination with Ramipril Global Endpoint Trial (ONTARGET). Measurements include date of presentation of ACE inhibitor-associated angioedema, population exposure to ACE inhibitor by date, and local pollen counts by date. RESULTS At Vanderbilt, the rate of angioedema was significantly associated with tree pollen months (P = .01 from χ2 test). When separate analyses were conducted in patients with a history of seasonal allergies and patients without, the rate of ACE inhibitor-associated angioedema was increased during tree pollen months only in patients with a history of seasonal allergies (P = .002). In Marshfield, the rate of angioedema was significantly associated with ragweed pollen months (P = .025). In ONTARGET, a positive trend was observed between the ACE inhibitor-associated angioedema rate and grass season, although it was not statistically significant (P = .057). CONCLUSIONS Patients with ACE inhibitor-associated angioedema are more likely to present with this adverse drug event during months when pollen counts are increased. PMID:24565618

  11. Models to predict the start of the airborne pollen season.

    PubMed

    Siniscalco, Consolata; Caramiello, Rosanna; Migliavacca, Mirco; Busetto, Lorenzo; Mercalli, Luca; Colombo, Roberto; Richardson, Andrew D

    2015-07-01

    Aerobiological data can be used as indirect but reliable measures of flowering phenology to analyze the response of plant species to ongoing climate changes. The aims of this study are to evaluate the performance of several phenological models for predicting the pollen start of season (PSS) in seven spring-flowering trees (Alnus glutinosa, Acer negundo, Carpinus betulus, Platanus occidentalis, Juglans nigra, Alnus viridis, and Castanea sativa) and in two summer-flowering herbaceous species (Artemisia vulgaris and Ambrosia artemisiifolia) by using a 26-year aerobiological data set collected in Turin (Northern Italy). Data showed a reduced interannual variability of the PSS in the summer-flowering species compared to the spring-flowering ones. Spring warming models with photoperiod limitation performed best for the greater majority of the studied species, while chilling class models were selected only for the early spring flowering species. For Ambrosia and Artemisia, spring warming models were also selected as the best models, indicating that temperature sums are positively related to flowering. However, the poor variance explained by the models suggests that further analyses have to be carried out in order to develop better models for predicting the PSS in these two species. Modeling the pollen season start on a very wide data set provided a new opportunity to highlight the limits of models in elucidating the environmental factors driving the pollen season start when some factors are always fulfilled, as chilling or photoperiod or when the variance is very poor and is not explained by the models. PMID:25234751

  12. Models to predict the start of the airborne pollen season

    NASA Astrophysics Data System (ADS)

    Siniscalco, Consolata; Caramiello, Rosanna; Migliavacca, Mirco; Busetto, Lorenzo; Mercalli, Luca; Colombo, Roberto; Richardson, Andrew D.

    2015-07-01

    Aerobiological data can be used as indirect but reliable measures of flowering phenology to analyze the response of plant species to ongoing climate changes. The aims of this study are to evaluate the performance of several phenological models for predicting the pollen start of season (PSS) in seven spring-flowering trees ( Alnus glutinosa, Acer negundo, Carpinus betulus, Platanus occidentalis, Juglans nigra, Alnus viridis, and Castanea sativa) and in two summer-flowering herbaceous species ( Artemisia vulgaris and Ambrosia artemisiifolia) by using a 26-year aerobiological data set collected in Turin (Northern Italy). Data showed a reduced interannual variability of the PSS in the summer-flowering species compared to the spring-flowering ones. Spring warming models with photoperiod limitation performed best for the greater majority of the studied species, while chilling class models were selected only for the early spring flowering species. For Ambrosia and Artemisia, spring warming models were also selected as the best models, indicating that temperature sums are positively related to flowering. However, the poor variance explained by the models suggests that further analyses have to be carried out in order to develop better models for predicting the PSS in these two species. Modeling the pollen season start on a very wide data set provided a new opportunity to highlight the limits of models in elucidating the environmental factors driving the pollen season start when some factors are always fulfilled, as chilling or photoperiod or when the variance is very poor and is not explained by the models.

  13. The effect of changes to the method of estimating the pollen count from aerobiological samples.

    PubMed

    Sikoparija, Branko; Pejak-Šikoparija, Tatjana; Radišić, Predrag; Smith, Matt; Soldevilla, Carmen Galán

    2011-02-01

    Pollen data have been recorded at Novi Sad in Serbia since 2000. The adopted method of producing pollen counts has been the use of five longitudinal transects that examine 19.64% of total sample surface. However, counting five transects is time consuming and so the main objective of this study is to investigate whether reducing the number to three or even two transects would have a significant effect on daily average and bi-hourly pollen concentrations, as well as the main characteristics of the pollen season and long-term trends. This study has shown that there is a loss of accuracy in daily average and bi-hourly pollen concentrations (an increase in % ERROR) as the sub-sampling area is reduced from five to three or two longitudinal transects. However, this loss of accuracy does not impact on the main characteristics of the season or long-term trends. As a result, this study can be used to justify changing the sub-sampling method used at Novi Sad from five to three longitudinal transects. The use of two longitudinal transects has been ruled out because, although quicker, the counts produced: (a) had the greatest amount of % ERROR, (b) altered the amount of influence of the independent variable on the dependent variable (the slope in regression analysis) and (c) the total sampled surface (7.86%) was less than the minimum requirement recommended by the European Aerobiology Society working group on Quality Control (at least 10% of total slide area). PMID:21135964

  14. Particulate matter modifies the association between airborne pollen and daily medical consultations for pollinosis in Tokyo.

    PubMed

    Konishi, Shoko; Ng, Chris Fook Sheng; Stickley, Andrew; Nishihata, Shinichi; Shinsugi, Chisa; Ueda, Kayo; Takami, Akinori; Watanabe, Chiho

    2014-11-15

    Pollen from Japanese cedar (sugi) and cypress (hinoki) trees is responsible for the growing prevalence of allergic rhinitis, especially pollinosis in Japan. Previous studies have suggested that air pollutants enhance the allergic response to pollen in susceptible individuals. We conducted a time-stratified case-crossover study to examine the potential modifying effects of PM2.5 and suspended particulate matter (SPM) on the association between pollen concentration and daily consultations for pollinosis. A total of 11,713 daily pollinosis cases (International Classification of Diseases, ICD-10, J30.1) from January to May, 2001-2011, were obtained from a clinic in Chiyoda, Tokyo. Daily pollen counts and the daily mean values of air pollutants (PM2.5, SPM, SO2, NO2, CO, and O3) were collected from monitoring stations across Tokyo. The effects of pollen were stratified by the level of PM2.5 and SPM to examine the interaction effect of pollen and particulate pollutants. We found a statistically significant interaction between pollen concentration and PM2.5/SPM. On days with a high level of PM2.5 (>95th percentile), an interquartile increase in the mean cumulative pollen count (an average of 28 pollen grains per cm(2) during lag-days 0 to 5) corresponded to a 10.30% (95%CI: 8.48%-12.16%) increase in daily new pollinosis cases, compared to 8.04% (95%CI: 7.28%-8.81%) on days with a moderate level of PM2.5 (5th-95th percentile). This interaction persisted when different percentile cut-offs were used and was robust to the inclusion of other air pollutants. A similar interaction pattern was observed between SPM and pollen when a less extreme cut-off for SPM was used to stratify the effect of pollen. Our study showed the acute effect of pollen was greater when the concentration of air particulate pollutant, specifically PM2.5 and SPM, was higher. These findings are consistent with the notion that particulate air pollution may act as an adjuvant that promotes allergic disease (i

  15. Correlation between airborne Olea europaea pollen concentrations and levels of the major allergen Ole e 1 in Córdoba, Spain, 2012-2014

    NASA Astrophysics Data System (ADS)

    Plaza, M. P.; Alcázar, P.; Galán, C.

    2016-04-01

    Olea europaea L. pollen is the second-largest cause of pollinosis in the southern Iberian Peninsula. Airborne-pollen monitoring networks provide essential data on pollen dynamics over a given study area. Recent research, however, has shown that airborne pollen levels alone do not always provide a clear indicator of actual exposure to aeroallergens. This study sought to evaluate correlations between airborne concentrations of olive pollen and Ole e 1 allergen levels in Córdoba (southern Spain), in order to determine whether atmospheric pollen concentrations alone are sufficient to chart changes in hay fever symptoms. The influence of major weather-related variables on local airborne pollen and allergen levels was also examined. Monitoring was carried out from 2012 to 2014. Pollen sampling was performed using a Hirst-type sampler, following the protocol recommended by the Spanish Aerobiology Network. A multi-vial cyclone sampler was used to collect aeroallergens, and allergenic particles were quantified by ELISA assay. Significant positive correlations were found between daily airborne allergen levels and atmospheric pollen concentrations, although there were occasions when allergen was detected before and after the pollen season and in the absence of airborne pollen. The correlation between the two was irregular, and pollen potency displayed year-on-year variations and did not necessarily match pollen-season-intensity.

  16. Masting in oaks: Disentangling the effect of flowering phenology, airborne pollen load and drought

    NASA Astrophysics Data System (ADS)

    Fernández-Martínez, Marcos; Belmonte, Jordina; Maria Espelta, Josep

    2012-08-01

    Quercus species exhibit an extreme inter-annual variability in seed production often synchronized over large geographical areas (masting). Since this reproductive behavior is mostly observed in anemophilous plants, pollination efficiency is suggested as one hypothesis to explain it, although resource-based hypotheses are also suggested as alternatives. We analyzed the effect of flowering phenology, airborne pollen presence and meteorological conditions in the pattern of acorn production in mixed evergreen-deciduous oak forests (Quercus ilex and Quercus pubescens) in NE Spain for twelve years (1998-2009). In both oaks, higher temperatures advanced the onset of flowering and increased the amount of airborne pollen. Nevertheless, inter-annual differences in pollen production did not influence acorn crop size. Acorn production was enhanced by a delay in flowering onset in Q. ilex but not in Q. pubescens. This suggests that in perennial oaks a larger number of photosynthates produced before flowering could benefit reproduction while the lack of effects on deciduous oaks could be because these species flush new leaves and flowers at the same time. Notwithstanding this effect, spring water deficit was the most relevant factor in explaining inter-annual variability in acorn production in both species. Considering that future climate scenarios predict progressive warmer and dryer spring seasons in the Mediterranean Basin, this might result in earlier onsets of flowering and higher water deficits that would constrain acorn production.

  17. Transport of airborne Picea schrenkiana pollen on the northern slope of Tianshan Mountains (Xinjiang, China) and its implication for paleoenvironmental reconstruction.

    PubMed

    Pan, Yanfang; Yan, Shun; Behling, Hermann; Mu, Guijin

    2013-06-01

    The understanding of airborne pollen transportation is crucial for the reconstruction of the paleoenvironment. Under favorable conditions, a considerable amount of long-distance-transported pollen can be deposited far from its place of origin. In extreme arid regions, in most cases, such situations occur and increase the difficulty to interpret fossil pollen records. In this study, three sets of Cour airborne pollen trap were installed on the northern slope of Tianshan Mountains to collect airborne Picea schrenkiana (spruce) pollen grains from July 2001 to July 2006. The results indicate that Picea pollen disperses extensively and transports widely in the lower atmosphere far away from spruce forest. The airborne Picea pollen dispersal period is mainly concentrated between mid-May and July. In desert area, weekly Picea pollen began to increase and peaked suddenly in concentration. Also, annual pollen indices do not decline even when the distance increased was probably related to the strong wind may pick up the deposited pollen grains from the topsoil into the air stream, leading to an increase of pollen concentration in the air that is irrelevant to the normal and natural course of pollen transport and deposition. This, in turn, may lead to erroneous interpretations of the pollen data in the arid region. This study provided insight into the shift in the Picea pollen season regarding climate change in arid areas. It is recorded that the pollen pollination period starts earlier and the duration became longer. The results also showed that the temperature of May and June was positively correlated with the Picea pollen production. Furthermore, the transport of airborne Picea pollen data is useful for interpreting fossil pollen records from extreme arid regions. PMID:23576840

  18. [Pollen counts (from Ambrosia and Artemisia) in Lyon-Bron from 1982 to 1985].

    PubMed

    Dechamp, C; Hoch, D; Chouraqui, M; Bensoussan, M; Dechamp, J

    1987-06-01

    The purpose of this work is to distinguish the broad outlines of the pollen calendar in the Lyons area for the 5th year, using the same method (P. COUR) and the same location--Lyon-Bron weather station. Weekly data is given for 1982, 1983, 1984, 1985 (1986 data was not complete at the time of the conference, October 1986). This work is the fruit of numerical counts integrated into a data processing program which enables pollens tested and not tested in allergology to be identified. In Lyon, we observe 3 pollen seasons: early Spring (TREES), Spring (TREES and GRAMINEAE) and Summer-Autumn (TREES, GRAMINEAE, and COMPOSITAE). During the late the following are present: Artemisia vulgaris (last 10 days of July), RAGWEED (mid-August-1st fortnight of October), Artemisia annua (end September-early October). The particularity of our region is that not only ragweed pollen is collected but also two categories of Mugwort pollen, at different periods. PMID:3454179

  19. Disentangling the effects of feedback structure and climate on Poaceae annual airborne pollen fluctuations and the possible consequences of climate change.

    PubMed

    García de León, David; García-Mozo, Herminia; Galán, Carmen; Alcázar, Purificación; Lima, Mauricio; González-Andújar, José L

    2015-10-15

    Pollen allergies are the most common form of respiratory allergic disease in Europe. Most studies have emphasized the role of environmental processes, as the drivers of airborne pollen fluctuations, implicitly considering pollen production as a random walk. This work shows that internal self-regulating processes of the plants (negative feedback) should be included in pollen dynamic systems in order to give a better explanation of the observed pollen temporal patterns. This article proposes a novel methodological approach based on dynamic systems to investigate the interaction between feedback structure of plant populations and climate in shaping long-term airborne Poaceae pollen fluctuations and to quantify the effects of climate change on future airborne pollen concentrations. Long-term historical airborne Poaceae pollen data (30 years) from Cordoba city (Southern Spain) were analyzed. A set of models, combining feedback structure, temperature and actual evapotranspiration effects on airborne Poaceae pollen were built and compared, using a model selection approach. Our results highlight the importance of first-order negative feedback and mean annual maximum temperature in driving airborne Poaceae pollen dynamics. The best model was used to predict the effects of climate change under two standardized scenarios representing contrasting temporal patterns of economic development and CO2 emissions. Our results predict an increase in pollen levels in southern Spain by 2070 ranging from 28.5% to 44.3%. The findings from this study provide a greater understanding of airborne pollen dynamics and how climate change might impact the future evolution of airborne Poaceae pollen concentrations and thus the future evolution of related pollen allergies. PMID:26026414

  20. Poaceae pollen in Galicia (N.W. Spain): characterisation and recent trends in atmospheric pollen season.

    PubMed

    Jato, V; Rodríguez-Rajo, F J; Seijo, M C; Aira, M J

    2009-07-01

    Airborne Poaceae pollen counts are greatly influenced by weather-related parameters, but may also be governed by other factors. Poaceae pollen is responsible for most allergic reactions in the pollen-sensitive population of Galicia (Spain), and it is therefore essential to determine the risk posed by airborne pollen counts. The global climate change recorded over recent years may prompt changes in the atmospheric pollen season (APS). This survey used airborne Poaceae pollen data recorded for four Galician cities since 1993, in order to characterise the APS and note any trends in its onset, length and severity. Pollen sampling was performed using Hirst-type volumetric traps; data were subjected to Spearman's correlation test and regression models, in order to detect possible correlations between different parameters and trends. The APS was calculated using ten different methods, in order to assess the influence of each on survey results. Finally, trends detected for the major weather-related parameters influencing pollen counts over the study period were compared with those recorded over the last 30 years. All four cities displayed a trend towards lower annual total Poaceae pollen counts, lower peak values and a smaller number of days on which counts exceeded 30, 50 and 100 pollen grains/m(3). Moreover, the survey noted a trend towards delayed onset and shorter duration of the APS, although differences were observed depending on the criteria used to define the first and the last day of the APS. PMID:19347372

  1. Poaceae pollen in Galicia (N.W. Spain): characterisation and recent trends in atmospheric pollen season

    NASA Astrophysics Data System (ADS)

    Jato, V.; Rodríguez-Rajo, F. J.; Seijo, M. C.; Aira, M. J.

    2009-07-01

    Airborne Poaceae pollen counts are greatly influenced by weather-related parameters, but may also be governed by other factors. Poaceae pollen is responsible for most allergic reactions in the pollen-sensitive population of Galicia (Spain), and it is therefore essential to determine the risk posed by airborne pollen counts. The global climate change recorded over recent years may prompt changes in the atmospheric pollen season (APS). This survey used airborne Poaceae pollen data recorded for four Galician cities since 1993, in order to characterise the APS and note any trends in its onset, length and severity. Pollen sampling was performed using Hirst-type volumetric traps; data were subjected to Spearman’s correlation test and regression models, in order to detect possible correlations between different parameters and trends. The APS was calculated using ten different methods, in order to assess the influence of each on survey results. Finally, trends detected for the major weather-related parameters influencing pollen counts over the study period were compared with those recorded over the last 30 years. All four cities displayed a trend towards lower annual total Poaceae pollen counts, lower peak values and a smaller number of days on which counts exceeded 30, 50 and 100 pollen grains/m3. Moreover, the survey noted a trend towards delayed onset and shorter duration of the APS, although differences were observed depending on the criteria used to define the first and the last day of the APS.

  2. Long-term monitoring of airborne pollen in Alaska and the Yukon: Possible implications for global change

    SciTech Connect

    Anderson, J.H.

    1992-03-01

    Airborne pollen and spores have been sampled since 1978 in Fairbanks and 1982 Anchorage and other Alaska-Yukon locations for medical and ecological purposes. Comparative analyses of pre- and post-1986 data subsets reveal that after 1986 (1) pollen is in the air earlier, (2) the multiyear average of degree-days promoting pollen onset is little changed while (3) annual variation in degree-days at onset is greater, (4) pollen and spore annual productions are considerably higher, and (5) there is more year-to-year variation in pollen production. These changes probably reflect directional changes in certain weather variables, and there is some indication that they are of global change significance, i.e., related to increasing atmospheric greenhouse gases. Correlations with pollen data suggest that weather variables of high influence are temperatures during specific periods following pollen dispersal in the preceding year and the average temperature in April of the current year. Annual variations in pollen dispersal might be roughly linked to the 11 year sunspot cycle through air temperature mediators. Weather in 1990, apparent pollen production cycles under endogenous control, and the impending sunspot maximum portend a very severe pollen season in 199 existing but unfunded sampling projects.

  3. A model for the determination of pollen count using google search queries for patients suffering from allergic rhinitis.

    PubMed

    König, Volker; Mösges, Ralph

    2014-01-01

    Background. The transregional increase in pollen-associated allergies and their diversity have been scientifically proven. However, patchy pollen count measurement in many regions is a worldwide problem with few exceptions. Methods. This paper used data gathered from pollen count stations in Germany, Google queries using relevant allergological/biological keywords, and patient data from three German study centres collected in a prospective, double-blind, randomised, placebo-controlled, multicentre immunotherapy study to analyse a possible correlation between these data pools. Results. Overall, correlations between the patient-based, combined symptom medication score and Google data were stronger than those with the regionally measured pollen count data. The correlation of the Google data was especially strong in the groups of severe allergy sufferers. The results of the three-centre analyses show moderate to strong correlations with the Google keywords (up to >0.8 cross-correlation coefficient, P < 0.001) in 10 out of 11 groups (three averaged patient cohorts and eight subgroups of severe allergy sufferers: high IgE class, high combined symptom medication score, and asthma). Conclusion. For countries with a good Internet infrastructure but no dense network of pollen traps, this could represent an alternative for determining pollen levels and, forecasting the pollen count for the next day. PMID:25045360

  4. Airborne pollen in three European cities: Detection of atmospheric circulation pathways by applying three-dimensional clustering of backward trajectories

    NASA Astrophysics Data System (ADS)

    Makra, LáSzló; SáNta, TamáS.; Matyasovszky, IstváN.; Damialis, Athanasios; Karatzas, Kostas; Bergmann, Karl-Christian; Vokou, Despoina

    2010-12-01

    The long-range transport of particulates can substantially contribute to local air pollution. The importance of airborne pollen has grown due to the recent climate change; the lengthening of the pollen season and rising mean airborne pollen concentrations have increased health risks. Our aim is to identify atmospheric circulation pathways influencing pollen levels in three European cities, namely Thessaloniki, Szeged, and Hamburg. Trajectories were computed using the HYSPLIT model. The 4 day, 6 hourly three-dimensional (3-D) backward trajectories arriving at these locations at 1200 UT are produced for each day over a 5 year period. A k-means clustering algorithm using the Mahalanobis metric was applied in order to develop trajectory types. The delimitation of the clusters performed by the 3-D function "convhull" is a novel approach. The results of the cluster analysis reveal that the main pathways for Thessaloniki contributing substantially to the high mean Urticaceae pollen levels cover western Europe and the Mediterranean. The key pathway patterns for Ambrosia for Szeged are associated with backward trajectories coming from northwestern Europe, northeastern Europe, and northern Europe. A major pollen source identified is a cluster over central Europe, namely the Carpathian basin with peak values in Hungary. The principal patterns for Poaceae for Hamburg include western Europe and the mid-Atlantic region. Locations of the source areas coincide with the main habitat regions of the species in question. Critical daily pollen number exceedances conditioned on the clusters were also evaluated using two statistical indices. An attempt was made to separate medium- and long-range airborne pollen transport.

  5. Airborne Pollen Concentrations and Emergency Room Visits for Myocardial Infarction: A Multicity Case-Crossover Study in Ontario, Canada.

    PubMed

    Weichenthal, Scott; Lavigne, Eric; Villeneuve, Paul J; Reeves, François

    2016-04-01

    Few studies have examined the acute cardiovascular effects of airborne allergens. We conducted a case-crossover study to evaluate the relationship between airborne allergen concentrations and emergency room visits for myocardial infarction (MI) in Ontario, Canada. In total, 17,960 cases of MI were identified between the months of April and October during the years 2004-2011. Daily mean aeroallergen concentrations (pollen and mold spores) were assigned to case and control periods using central-site monitors in each city along with daily measurements of meteorological data and air pollution (nitrogen dioxide and ozone). Odds ratios and their 95% confidence intervals were estimated using conditional logistic regression models adjusting for time-varying covariates. Risk of MI was 5.5% higher (95% confidence interval (CI): 3.4, 7.6) on days in the highest tertile of total pollen concentrations compared with days in the lowest tertile, and a significant concentration-response trend was observed (P < 0.001). Higher MI risk was limited to same-day pollen concentrations, with the largest risks being observed during May (odds ratio = 1.16, 95% CI: 1.00, 1.35) and June (odds ratio = 1.10, 95% CI: 1.00, 1.22), when tree and grass pollen are most common. Mold spore concentrations were not associated with MI. Our findings suggest that airborne pollen might represent a previously unidentified environmental risk factor for myocardial infarction. PMID:26934896

  6. Influence of atmospheric ozone, PM 10 and meteorological factors on the concentration of airborne pollen and fungal spores

    NASA Astrophysics Data System (ADS)

    Sousa, S. I. V.; Martins, F. G.; Pereira, M. C.; Alvim-Ferraz, M. C. M.; Ribeiro, H.; Oliveira, M.; Abreu, I.

    The increase of allergenic symptoms has been associated with air contaminants such as ozone, particulate matter, pollen and fungal spores. Considering the potential relevance of crossed effects of non-biological pollutants and airborne pollens and fungal spores on allergy worsening, the aim of this work was to evaluate the influence of non-biological pollutants and meteorological parameters on the concentrations of pollen and fungal spores using linear correlations and multiple linear regressions. For that, the seasonal variation of ozone, particulate matter with an equivalent aerodynamic diameter smaller than 10 μm, pollen and fungal spores were assessed and statistical correlations were analysed between those parameters. The data were collected through 2003-2005 in Porto, Portugal. The linear correlations showed that ozone and particulate matter had no significant influence on the concentration of pollen and fungal spores. On the contrary, when using multiple linear regressions those parameters showed to have some influence on the biological pollutants, although results were different depending on the year analysed. Among the meteorological parameters analysed, temperature was the one that most influenced the pollen and fungal spores airborne concentrations, both when using linear and multiple linear correlations. Relative humidity also showed to have some influence on the fungal spore dispersion when multiple linear regressions were used. Nevertheless, the conclusions for each pollen and fungal spore were different depending on the analysed period, which means that the correlations identified as statistically significant may not be, even so, consistent enough. Furthermore, the comparison of the results here presented with those obtained by other authors for only one period should be made carefully.

  7. The long distance transport of airborne Ambrosia pollen to the UK and the Netherlands from Central and south Europe

    NASA Astrophysics Data System (ADS)

    de Weger, Letty A.; Pashley, Catherine H.; Šikoparija, Branko; Skjøth, Carsten A.; Kasprzyk, Idalia; Grewling, Łukasz; Thibaudon, Michel; Magyar, Donat; Smith, Matt

    2016-04-01

    The invasive alien species Ambrosia artemisiifolia (common or short ragweed) is increasing its range in Europe. In the UK and the Netherlands, airborne concentrations of Ambrosia pollen are usually low. However, more than 30 Ambrosia pollen grains per cubic metre of air (above the level capable to trigger allergic symptoms) were recorded in Leicester (UK) and Leiden (NL) on 4 and 5 September 2014. The aims of this study were to determine whether the highly allergenic Ambrosia pollen recorded during the episode could be the result of long distance transport, to identify the potential sources of these pollen grains and to describe the conditions that facilitated this possible long distance transport. Airborne Ambrosia pollen data were collected at 10 sites in Europe. Back trajectory and atmospheric dispersion calculations were performed using HYSPLIT_4. Back trajectories calculated at Leicester and Leiden show that higher altitude air masses (1500 m) originated from source areas on the Pannonian Plain and Ukraine. During the episode, air masses veered to the west and passed over the Rhône Valley. Dispersion calculations showed that the atmospheric conditions were suitable for Ambrosia pollen released from the Pannonian Plain and the Rhône Valley to reach the higher levels and enter the airstream moving to northwest Europe where they were deposited at ground level and recorded by monitoring sites. The study indicates that the Ambrosia pollen grains recorded during the episode in Leicester and Leiden were probably not produced by local sources but transported long distances from potential source regions in east Europe, i.e. the Pannonian Plain and Ukraine, as well as the Rhône Valley in France.

  8. Pollen Allergy

    MedlinePlus

    ... pollen count, which is often reported by local weather broadcasts or allergy websites, is a measure of how much pollen is in the air. Pollen counts tend to be highest early in the morning on warm, dry, breezy days and lowest during chilly, wet periods. ...

  9. Airborne-pollen pool and mating pattern in a hybrid zone between Pinus pumila and P. parviflora var. pentaphylla.

    PubMed

    Ito, Megumi; Suyama, Yoshihisa; Ohsawa, Takeshi A; Watano, Yasuyuki

    2008-12-01

    The reproductive isolation barriers and the mating patterns among Pinus pumila, P. parviflora var. pentaphylla and their hybrids were examined by flowering phenology and genetic assays of three life stages: airborne-pollen grains, adults and seeds, in a hybrid zone on Mount Apoi, Hokkaido, Japan. Chloroplast DNA composition of the airborne-pollen was determined by single-pollen polymerase chain reaction. Mating patterns were analysed by estimating the molecular hybrid index of the seed parent, their seed embryos and pollen parents. The observation of flowering phenology showed that the flowering of P. pumila precedes that of P. parviflora var. pentaphylla by about 6 to 10 days within the same altitudinal ranges. Although this prezygotic isolation barrier is effective, the genetic assay of airborne-pollen showed that the two pine species, particularly P. pumila, still have chances to form F(1) hybrid seeds. Both parental species showed a strong assortative mating pattern; F(1) seeds were found in only 1.4% of seeds from P. pumila mother trees and not at all in P. parviflora var. pentaphylla. The assortative mating was concluded as the combined result of flowering time differentiation and cross-incompatibility. In contrast to the parental species, hybrids were fertilized evenly by the two parental species and themselves. The breakdown of prezygotic barriers (intermediate flowering phenology) and cross-incompatibility may account for the unselective mating. It is suggested that introgression is ongoing on Mount Apoi through backcrossing between hybrids and parental species, despite strong isolation barriers between the parental species. PMID:19120991

  10. Airborne pollen-climate relationship based on discriminant analysis in Nam Co, Central Tibet and its palaeoenvironmental significance

    NASA Astrophysics Data System (ADS)

    Lyu, X.; Zhu, L.; Ma, Q.; Li, Q.

    2014-12-01

    Based on the airborne pollen data collected using a Burkard pollen trap, discriminant analysis were conducted to evaluate the relationship between two different atmospheric circulation systems, the Asia summer monsoon (ASM) and the Westerlies, in Nam Co basin, central Tibet. The whole year's samples could be classified into two groups using cluster analysis: one group was from May to September, another group was from October to April of next year, corresponding to monsoon period and non-monsoon period, respectively. The classification represents two different atmospheric circulation systems, ASM in monsoon period and the Westerlies in non-monsoon period. Discriminant analysis was performed. First, the whole year samples were divided into two a priori groups, group A is monsoon period (May-Sep.) and group B is non-monsoon period (Oct.-Apr.). Then percentage data of major pollen taxa were used to establish the discriminant functions, and then the samples were classified into predicted groups. The results of discriminant analysis show that 78.6% of the samples were cross-validated grouped correctly. Thus, airborne pollen assemblages can distinguish two different climate systems: monsoon period and non-monsoon period. According to the discriminant score, the group centroids of group A and group B were negative and positive, respectively. Therefore, we created the discriminant score as a new monsoon index (PDI, Pollen Discriminant Index), small PDI values represented enhanced summer monsoon climate. Using above result, we calculated the PDI of Nam Co NCL core, the PDI values can be coincided with Dryness (moisture indicator) and A/Cy ratio (temperature indicator).

  11. Contribution of particle counting in assessment of exposure to airborne microorganisms

    NASA Astrophysics Data System (ADS)

    Parat, Sylvie; Perdrix, Alain; Mann, Sylvie; Baconnier, Pierre

    The aim of the study was to determine the relationship between airborne bacterial concentrations and particle counts measured simultaneously at different sites. Andersen single stage viable particle samplers were used for microbial measurements while a Laser particle counter gave the cumulated counts of particles larger than 0.5 μm diameter. The first phase of the study was performed in two experimental rooms where the basic level of microbial contamination was low. Peaks of concentrations were generated by human activity and both bacterial and particle counts were monitored over 1 h. In the second phase, measurements were run for several days in three different buildings normally occupied. Natural variations of bacterial and particle counts were monitored: microbial measurements were performed each hour while particle counts were started with a 10 min frequency. Statistics revealed strong positive correlations between bacterial and particle counts in four sites out of five. Analyses of covariance used to compare the regression lines obtained in each area showed that except for two natural sites, the regression lines were significantly different, indicating that no absolute relationship can be established between the two parameters. Therefore, particle counting should, of course, not take the place of microorganism measurements, but combining particle counting with bioaerosols measurements may allow detection of rapid variations instantaneously and indicate further microbial measurements. This strategy should improve the assessment of people"s real exposure to airborne microorganisms.

  12. Total airborne mold particle sampling: evaluation of sample collection, preparation and counting procedures, and collection devices.

    PubMed

    Godish, Diana; Godish, Thad

    2008-02-01

    This study was conducted to evaluate (i) procedures used to collect, prepare, and count total airborne mold spore/particle concentrations, and (ii) the relative field performance of three commercially available total airborne mold spore/particle sampling devices. Differences between factory and laboratory airflow calibration values of axial fan-driven sampling instruments (used in the study) indicated a need for laboratory calibration using a mass flow meter to ensure that sample results were accurately calculated. An aniline blue-amended Calberla's solution adjusted to a pH of 4.2-4.4 provided good sample mounting/counting results using Dow Corning high vacuum grease, Dow Corning 280A adhesive, and Dow Corning 316 silicone release spray for samples collected using mini-Burkard and Allergenco samplers. Count variability among analysts was most pronounced in 5% counts of relatively low mold particle deposition density samples and trended downward with increased count percentage and particle deposition density. No significant differences were observed among means of 5, 10, and 20% counts and among analysts; a significant interaction effect was observed between analysts' counts and particle deposition densities. Significantly higher mini-Burkard and Air-O-Cell total mold spore/particle counts for 600x vs. 400x (1.9 and 2.3 x higher, respectively), 1000x vs. 600x (1.9 and 2.2 x higher, respectively) and 1000x vs. 400x (3.6 and 4.6 x higher, respectively) comparisons indicated that 1000x magnification counts best quantified total airborne mold spore/particles using light microscopy, and that lower magnification counts may result in unacceptable underreporting of airborne mold spore/particle concentrations. Modest but significantly higher (1.2x) total mold spore concentrations were observed with Allergenco vs. mini-Burkard samples collected in co-located, concurrently operated sampler studies; moderate but significantly higher mini-Burkard count values (1.4x) were

  13. Seasonal variations of nasal resistance in allergic rhinitis and environmental pollen counts. III: Efficacy of antiallergic agents.

    PubMed

    Naito, K; Senoh, Y; Ishihara, M; Takeda, N; Ibata, K; Yokoyama, N; Iwata, S

    1994-01-01

    We previously reported that, in a patient with Japanese cedar pollinosis, preseasonal increase in total nasal airway resistance (NAR) possibly caused from circannual endogenous rhythm was suppressed by preseasonal systemic administration of antiallergic agent. In this study, in order to determine the influence of preseasonal use of a topical antiallergic agent and seasonal administration of a peroral antiallergic agent, we made measurement of nasal airway resistance and pollen counts under the previous respective conditions in separated years. Preseasonal high mean values of total NAR for a couple of weeks was not suppressed by the preseasonal topical administration of ketotifen nasal spray in 1992 which was a low pollen season (974/cm2). However, the patient did not complained of severe episodes of hay fever at all throughout the season. Preseasonal increases in mean values of total NAR were observed in 1993 which was a high Japanese cedar pollen season (4,875/cm2) with NAR the same as untreated seasons. This may have been because the patient was not treated with preseasonal use of peroral azelastine. On extremely high Japanese cedar pollen count days further increased NAR occurred in the patient. The patient experienced relatively severe hay fever episodes, but used no other anti-histamine nor topical steroid during the season. PMID:7872891

  14. Airborne pollen assemblages and weather regime in the central-eastern Loess Plateau, China

    NASA Astrophysics Data System (ADS)

    Li, Yuecong; Ge, Yawen; Xu, Qinghai; Bunting, Jane M.; Lv, Suqing; Wang, Junting; Li, Zetao

    2015-04-01

    This paper presents the results of pollen trapping studies designed to quantify the pollen assemblages carried in the winds of the Loess Plateau in Luochuan and Hunyuan. The one-year-collection samples analysis results show that pollen assemblages can be more sensitive to the change of climate than the vegetation composition, because of the change of pollen production. The analysis results of pollen traps in different weather regimes indicate that the pollen influx coming from dust weather contribute more to the total pollen influx than that coming from non-dust weather. The wind speed is the most important influenced factor to pollen assemblages, then the mean temperature and the mean relative humidity, the wind direction also contributes some. Strong wind coming from dust direction can make the percent and influx of Artemisia and Chenopodiaceae increase obviously with averagely higher than over 2.7 times in dust weather than in non-dust samples. The influences of wind speed and wind direction are not serious to some arboreal pollen such as Rosaceae, Quercus, Betula, Pinus and Ostryopsis, which are mainly influenced by temperature or the relative humidity such as Salix, Hippophae, Carpinus, Brassicaceae, Cupressaceae, Fabaceae.

  15. Can Twitter Be a Source of Information on Allergy? Correlation of Pollen Counts with Tweets Reporting Symptoms of Allergic Rhinoconjunctivitis and Names of Antihistamine Drugs.

    PubMed

    Gesualdo, Francesco; Stilo, Giovanni; D'Ambrosio, Angelo; Carloni, Emanuela; Pandolfi, Elisabetta; Velardi, Paola; Fiocchi, Alessandro; Tozzi, Alberto E

    2015-01-01

    Pollen forecasts are in use everywhere to inform therapeutic decisions for patients with allergic rhinoconjunctivitis (ARC). We exploited data derived from Twitter in order to identify tweets reporting a combination of symptoms consistent with a case definition of ARC and those reporting the name of an antihistamine drug. In order to increase the sensitivity of the system, we applied an algorithm aimed at automatically identifying jargon expressions related to medical terms. We compared weekly Twitter trends with National Allergy Bureau weekly pollen counts derived from US stations, and found a high correlation of the sum of the total pollen counts from each stations with tweets reporting ARC symptoms (Pearson's correlation coefficient: 0.95) and with tweets reporting antihistamine drug names (Pearson's correlation coefficient: 0.93). Longitude and latitude of the pollen stations affected the strength of the correlation. Twitter and other social networks may play a role in allergic disease surveillance and in signaling drug consumptions trends. PMID:26197474

  16. Environmental contamination and airborne microbial counts: a role for hydroxyl radical disinfection units?

    PubMed

    Wong, V; Staniforth, K; Boswell, T C

    2011-07-01

    Environmental contamination is thought to play a role in the spread of infection in hospitals and there has been increased interest in novel air disinfection systems in preventing infection. In this study the efficacy of a hydroxyl radical air disinfection system (Inov8 unit) in reducing the number of airborne bacteria was assessed in a clinical setting. Environmental contamination was assessed using settle plates and air samples in three settings: (1) non-clinical room; (2) non-clinical room with defined activity; and (3) single intensive care unit cubicle. A comparison of air counts and environmental contamination rates was made with the Inov8 units on and off. The Inov8 unit produced an overall reduction in both air sample and settle plate counts in each setting (P<0.001, Wilcoxon signed-rank test). There was a mean reduction in air sample counts of 26%, 39% and 55% for settings 1, 2 and 3 respectively. The corresponding reductions in settle plate counts were 35%, 62% and 54%. These results suggest that this type of novel air disinfection may have a role in improving air quality and reducing environmental contamination within clinical isolation rooms. Further work is required to assess the effect on specific pathogens, and to establish whether this will reduce the risks of patients and/or healthcare workers acquiring such pathogens from the environment. PMID:21497944

  17. Variations and trends of Fagaceae pollen in Northern Sardinia, Italy

    NASA Astrophysics Data System (ADS)

    Canu, Annalisa; Pellizzaro, Grazia; Arca, Bachisio; Vargiu, Arnoldo

    2016-04-01

    The aim of this study is to analyze variations in the start and the end dates of pollen season, date of maximum concentration peak, pollen season duration, pollen concentration value and Seasonal Pollen Index of airborne Fagaceae pollen series recorded in Sassari, Northern Italy, and to evaluate their relation to meteorological data. Daily pollen concentration data were measured from 1986 to 2008 in a urban area of northern Sardinia (Italy) using a Burkard seven-day recording volumetric spore trap. The date of the peak occurrence was defined as the day when the cumulated daily pollen values reached the 50 % of the total annual pollen concentration. Meteorological data were recorded during the same period by an automatic weather station. Cumulative Degree days were calculated, for each year, from different starting dates using the daily averaging method. The correlation between meteorological variables and the different characteristics of pollen seasons was analyzed using Spearman's correlation tests. In the city of Sassari the Fagaceae airborne pollen content was mainly due to Quercus. The main pollen season took place from April to June. The longest pollen season appeared in the year 2002. The cumulative counts varied over the years, with a mean value of 5,336 pollen grains, a lowest total of 550 in 1986 and a highest total of 8,678 in 2001. Daily pollen concentrations presented positive correlation with temperature, and negative with relative humidity (p<0,0001) and with rainfall. In addition, Cumulative Degree days were significantly correlated with the dates of maximum concentration peak (p<0,0001).

  18. Airborne pollen sampling in Manoa Valley, Hawaii: effect of rain, humidity and wind.

    PubMed

    Massey, D G; Fournier-Massey, G

    1984-05-01

    Kramer-Collins pollen sampling was conducted over 24 hours for 25 consecutive months at two valley sites in Honolulu. Of 1,059 expected samples, 699 (66.0%) were collected. Only 25 were considered excellent, i.e., eight three-hour collection bands. Twenty eight were considered good, ie., two to six bands. The difficulties in the study were associated with the weather directly (17.5%), the power source (3.9%), inadequancy of the samplers (63.1%) and the inexperience of technicians (15.3%). Sampler problems were also indirectly attributable to the high humidity, rain and wind, which differed at the two sites. PMID:6721258

  19. Aerobiological studies and low allerginicity of date-palm pollen in the UAE.

    PubMed

    Almehdi, Ahmed M; Maraqa, Munjed; Abdulkhalik, Samar

    2005-06-01

    Date-Palm trees (Phoenix dactylifera L.) are the most abundant crop in the United Arab Emirates (UAE). The aim of this work was to conduct aerobiological studies on Date-Palm pollens and correlate that to allergenicity. An aerobiological survey was performed at three Date-Palm farms. Radioallergosorbent test (RAST) and total IgE were performed on 477 airborne allergic patients. Small mass bioactive constituents were fractionated and isolated by HPLC. Aerobiological studies demonstrate the short distance traveled by the Date-Palm pollens. Pollen counts were about 800 counts/m3 within the Date-Palm farms and decreased by about 80% just 100 meters away from the farm area and almost diminished beyond 200 meters. Scanning electron micrograph of the pollen grain showed a uniform smooth texture with an oval shape. Out of 477 airborne allergic patients having high total IgE counts, only 2.3% gave positive RAST for Date-Palm pollen. HPLC chromatogram separated the non-protein content of Date-Palm pollen into four distinct peak fractions. The present study revealed that Date-Palm pollens have a low allergic effect on airborne allergic people. The short distance traveled by the pollen, the smooth texture, the short pollination period and low molecular weight biomolecule content may be the main factors behind the low allergenicity. PMID:16134484

  20. Characterization of Pollen Dispersion in the Neighborhood of Tokyo, Japan in the Spring of 2005 and 2006

    PubMed Central

    Ishibashi, Yoshinaga; Ohno, Hideki; Oh-ishi, Shuji; Matsuoka, Takeshi; Kizaki, Takako; Yoshizumi, Kunio

    2008-01-01

    The behavior of Japanese cedar (Cryptomeria japonica) and Japanese cypress (Chamaecyparis obtusa) pollens in an urban area was examined through the measurements of the dispersion characteristics at the various sampling locations in both outdoor and indoor environments. Airborne pollens were counted continuously for three months during the Japanese cedar pollen and Japanese cypress seasons in 2005 and 2006 by the use of Durham’s pollen trap method in and around Tokyo, Japan. The dispersion of pollens at the rooftop of Kyoritsu Women’s University was observed to be at extremely high levels in 2005 compared with previously reported results during the past two decades. As for Japanese cedar pollen, the maximum level was observed as 440 counts cm−2 day−1 on 18 March 2005. Japanese cypress pollen dispersed in that area in the latter period was compared with the Japanese cedar pollen dispersions. The maximum dispersion level was observed to be 351 counts cm−2 day−1 on 7 April 2005. Total accumulated dispersions of Japanese cedar and Japanese cypress pollens were 5,552 and 1,552 counts cm−2 for the three months (Feb., Mar. and Apr.) in 2005, respectively. However, the dispersion of both pollens in 2006 was very low. The total accumulated dispersions of Japanese cedar and Japanese cypress pollens were 421 and 98 counts cm−2 for three months (Feb., Mar. and Apr.) in 2006, respectively. Moreover, the pollen deposition on a walking person in an urban area showed that the pollen counts on feet were observed to be extremely high compared with the ones on the shoulder, back and legs. These findings suggested that pollen fell on the surface of the paved road at first, rebounded to the ambient air and was deposited on the residents again. Furthermore, the regional distribution of the total pollen dispersion in the South Kanto area was characterized on 15–16 March 2005 and on 14–15 March 2006. Although the pollen levels in 2005 were much higher than in 2006, it

  1. Photon-counting multikilohertz microlaser altimeters for airborne and spaceborne topographic measurements

    NASA Astrophysics Data System (ADS)

    Degnan, John J.

    2002-11-01

    We consider the optimum design of photon-counting microlaser altimeters operating from airborne and spaceborne platforms under both day and night conditions. Extremely compact, passively Q-switched microlaser transmitters produce trains of low energy pulses at multi-kHz rates and can easily generate subnanosecond pulsewidths for precise ranging. To guide the design, we have modeled the solar noise background and developed simple algorithms, based on post-detection Poisson filtering (PDPF), to optimally extract the weak altimeter signal from a high noise background during daytime operations. The advantages of photon-counting detector arrays followed by multichannel timing receivers for high resolution topographic mapping are discussed. Practical technology issues, such as detector and/or receiver dead times and their impact on signal detection and ranging accuracy and resolution, have also been considered in the analysis. The theoretical results are reinforced by data from an airborne microlaser altimeter, developed under NASA's Instrument Incubator Program. The latter instrument has operated at several kHz rates from aircraft cruise altitudes up to 6.7 km with laser pulse energies on the order of a few microjoules. The instrument has successfully recorded decimeter accuracy or better single photon returns from man-made structures, tree canopies and underlying terrain and has demonstrated shallow water bathymetry at depths to a few meters. We conclude the discussion by analyzing a photon counting instrument designed to produce, over a mission life of 3 years, a globally contiguous map of the Martian surface, with 5 m horizontal resolution and decimeter vertical accuracy, from an altitude of 300 km. The transmitter power-receive aperture product required is comparable to the Geoscience Laser Altimeter System (GLAS) but the number of individual range measurements to the surface is increased by three to four orders of magnitude. For more modest scientific goals, on a

  2. Design and performance of a fiber array coupled multi-channel photon counting, 3D imaging, airborne lidar system

    NASA Astrophysics Data System (ADS)

    Huang, Genghua; Shu, Rong; Hou, Libing; Li, Ming

    2014-06-01

    Photon counting lidar has an ultra-high sensitivity which can be hundreds even thousands of times higher than the linear detection lidar. It can significantly increase the system's capability of detection rang and imaging density, saving size and power consumings in airborne or space-borne applications. Based on Geiger-mode Si avalanche photodiodes (Si-APD), a prototype photon counting lidar which used 8 APDs coupled with a 1×8-pixel fiber array has been made in June, 2011. The experiments with static objects showed that the photon counting lidar could operate in strong solar background with 0.04 receiving photoelectrons on average. Limited by less counting times in moving platforms, the probability of detection and the 3D imaging density would be lower than that in static platforms. In this paper, a latest fiber array coupled multi-channel photon counting, 3D imaging, airborne lidar system is introduced. The correlation range receiver algorithm of photon counting 3D imaging is improved for airborne signal photon events extraction and noise filter. The 3D imaging experiments in the helicopter shows that the false alarm rate is less than 6×10-7, and the correct rate is better than 99.9% with 4 received photoelectrons and 0.7MHz system noise on average.

  3. An automated particle counting immunoassay (PACIA) for determination of blocking antibodies against timothy grass pollen in sera from desensitized allergics.

    PubMed

    Djurup, R; Magnusson, C G; Minuva, U; Søndergaard, I; Osterballe, O; Weeke, B

    1983-04-01

    An automated particle counting immunoassay (PACIA) for measurement of blocking antibodies (antigen neutralizing capacity) against timothy grass pollen extract in sera from desensitized allergics is described. Latex particles coated with F(ab')2-anti-timothy are agglutinated by timothy. Serum containing anti-timothy antibodies inhibits the agglutination. Non-agglutinated particles are counted in a modified AutoCounter. Nineteen of 20 sera from timothy allergics who had undergone immunotherapy with purified timothy extract for 30 weeks, showed significant agglutination-inhibition. None of 42 normal human sera gave significant inhibition. The inhibiting antibody could be removed by absorption with protein A and was thus of non-IgE nature, i.e. blocking antibody. The results obtained correlated statistically significantly with those found with a double-antibody method (rS = 0.62, n = 20, t = 3.35, P less than 0.01) and with the cumulated dosage of timothy allergen extract administered to the individual patient (rS = 0.56, n = 20, t = 2.87, P less than 0.02). Between-assay coefficient of variation was from 6.4% to 18.3%. The capacity is 40 samples per hour. The method has also been applied to measurement of blocking antibodies to honey bee and wasp venom. PMID:6846744

  4. Bee Pollen

    MedlinePlus

    ... Don’t confuse bee pollen with bee venom, honey, or royal jelly. People take bee pollen for ... Pollen, Extrait de Pollen d’Abeille, Honeybee Pollen, Honey Bee Pollen, Maize Pollen, Pine Pollen, Polen de ...

  5. The Slope Imaging Multi-polarization Photon-counting Lidar: an Advanced Technology Airborne Laser Altimeter

    NASA Astrophysics Data System (ADS)

    Dabney, P.; Harding, D. J.; Huss, T.; Valett, S.; Yu, A. W.; Zheng, Y.

    2009-12-01

    The Slope Imaging Multi-polarization Photon-counting Lidar (SIMPL) is an airborne laser altimeter developed through the NASA Earth Science Technology Office Instrument Incubator Program with a focus on cryopshere remote sensing. The SIMPL instrument incorporates a variety of advanced technologies in order to demonstrate measurement approaches of potential benefit for improved airborne laser swath mapping and spaceflight laser altimeter missions. SIMPL incorporates beam splitting, single-photon ranging and polarimetry technologies at green and near-infrared wavelengths in order to achieve simultaneous sampling of surface elevation, slope, roughness and scattering properties, the latter used to differentiate surface types. The transmitter is a 1 nsec pulse width, 11 kHz, 1064 nm microchip laser, frequency doubled to 532 nm and split into four plane-polarized beams using birefringent calcite crystal in order to maintain co-alignment of the two colors. The 16 channel receiver splits the received energy for each beam into the two colors and each color is split into energy parallel and perpendicular to the transmit polarization plane thereby proving a measure of backscatter depolarization. The depolarization ratio is sensitive to the proportions of specular reflection and surface and volume scattering, and is a function of wavelength. The ratio can differentiate, for example, water, young translucent ice, older granular ice and snow. The solar background count rate is controlled by spatial filtering using a pinhole array and by spectral filtering using temperature-controlled narrow bandwidth filters. The receiver is fiber coupled to 16 Single Photon Counting Modules (SPCMs). To avoid range biases due to the long dead time of these detectors the probability of detection per laser fire on each channel is controlled to be below 30%, using mechanical irises and flight altitude. Event timers with 0.1 nsec resolution in combination the narrow transmit pulse yields single

  6. Photon-Counting Multikilohertz Microlaser Altimeters for Airborne and Spaceborne Topographic Measurements

    NASA Technical Reports Server (NTRS)

    Degnan, John J.; Smith, David E. (Technical Monitor)

    2000-01-01

    We consider the optimum design of photon-counting microlaser altimeters operating from airborne and spaceborne platforms under both day and night conditions. Extremely compact Q-switched microlaser transmitters produce trains of low energy pulses at multi-kHz rates and can easily generate subnanosecond pulse-widths for precise ranging. To guide the design, we have modeled the solar noise background and developed simple algorithms, based on Post-Detection Poisson Filtering (PDPF), to optimally extract the weak altimeter signal from a high noise background during daytime operations. Practical technology issues, such as detector and/or receiver dead times, have also been considered in the analysis. We describe an airborne prototype, being developed under NASA's instrument Incubator Program, which is designed to operate at a 10 kHz rate from aircraft cruise altitudes up to 12 km with laser pulse energies on the order of a few microjoules. We also analyze a compact and power efficient system designed to operate from Mars orbit at an altitude of 300 km and sample the Martian surface at rates up to 4.3 kHz using a 1 watt laser transmitter and an 18 cm telescope. This yields a Power-Aperture Product of 0.24 W-square meter, corresponding to a value almost 4 times smaller than the Mars Orbiting Laser Altimeter (0. 88W-square meter), yet the sampling rate is roughly 400 times greater (4 kHz vs 10 Hz) Relative to conventional high power laser altimeters, advantages of photon-counting laser altimeters include: (1) a more efficient use of available laser photons providing up to two orders of magnitude greater surface sampling rates for a given laser power-telescope aperture product; (2) a simultaneous two order of magnitude reduction in the volume, cost and weight of the telescope system; (3) the unique ability to spatially resolve the source of the surface return in a photon counting mode through the use of pixellated or imaging detectors; and (4) improved vertical and

  7. Allergenic pollen season variations in the past two decades under changing climate in the United States

    PubMed Central

    Zhang, Yong; Bielory, Leonard; Mi, Zhongyuan; Cai, Ting; Robock, Alan; Georgopoulos, Panos

    2014-01-01

    Many diseases are linked with climate trends and variations. In particular, climate change is expected to alter the spatiotemporal dynamics of allergenic airborne pollen and potentially increase occurrence of allergic airway disease. Understanding the spatiotemporal patterns of changes in pollen season timing and levels is thus important in assessing climate impacts on aerobiology and allergy caused by allergenic airborne pollen. Here we describe the spatiotemporal patterns of changes in the seasonal timing and levels of allergenic airborne pollen for multiple taxa in different climate regions at a continental scale. The allergenic pollen seasons of representative trees, weeds and grass during the past decade (2001–2010) across the contiguous United States have been observed to start 3.0 (95% Confidence Interval (CI), 1.1–4.9) days earlier on average than in the 1990s (1994–2000). The average peak value and annual total of daily counted airborne pollen have increased by 42.4% (95% CI, 21.9%–62.9%) and 46.0% (95% CI, 21.5%–70.5%), respectively. Changes of pollen season timing and airborne levels depend on latitude, and are associated with changes of growing degree days, frost free days, and precipitation. These changes are likely due to recent climate change and particularly the enhanced warming and precipitation at higher latitudes in the contiguous United States. PMID:25266307

  8. Can Twitter Be a Source of Information on Allergy? Correlation of Pollen Counts with Tweets Reporting Symptoms of Allergic Rhinoconjunctivitis and Names of Antihistamine Drugs

    PubMed Central

    Gesualdo, Francesco; Stilo, Giovanni; D’Ambrosio, Angelo; Carloni, Emanuela; Pandolfi, Elisabetta; Velardi, Paola; Fiocchi, Alessandro; Tozzi, Alberto E.

    2015-01-01

    Pollen forecasts are in use everywhere to inform therapeutic decisions for patients with allergic rhinoconjunctivitis (ARC). We exploited data derived from Twitter in order to identify tweets reporting a combination of symptoms consistent with a case definition of ARC and those reporting the name of an antihistamine drug. In order to increase the sensitivity of the system, we applied an algorithm aimed at automatically identifying jargon expressions related to medical terms. We compared weekly Twitter trends with National Allergy Bureau weekly pollen counts derived from US stations, and found a high correlation of the sum of the total pollen counts from each stations with tweets reporting ARC symptoms (Pearson’s correlation coefficient: 0.95) and with tweets reporting antihistamine drug names (Pearson’s correlation coefficient: 0.93). Longitude and latitude of the pollen stations affected the strength of the correlation. Twitter and other social networks may play a role in allergic disease surveillance and in signaling drug consumptions trends. PMID:26197474

  9. Incidence of Betulaceae pollen and pollinosis in Zagreb, Croatia, 2002-2005.

    PubMed

    Peternel, Renata; Milanović, Sanja Musić; Hrga, Ivana; Mileta, Tatjana; Culig, Josip

    2007-01-01

    Pollen allergy is characterized by seasonal allergic manifestations affecting patients during the plant pollen season. The aim of this study was to analyze the Betulaceae pollen pattern in Zagreb (2002-2005) and to determine the incidence of sensitization to these pollen types in patients with seasonal respiratory allergy. Twenty-four-hour pollen counts were carried out using volumetric procedure. Skin prick test were performed on a total of 864 patients aged 18-80< in Zagreb between 2 January-31 December 2004. Pollen of the representatives of the family Betulaceae accounted for a significant proportion of total pollen (34% on an average), predominated by Betula pollen and considerably lower proportion of Alnus sp. and Corylus sp. pollen. Alder and hazel pollen first occurred in the air in February throughout the study period. The highest airborne pollen concentration of these taxa was recorded in February and March. The birch pollen season generally peaked in April. Only 2.67% of patients showed birch pollen monosensitization. The proportion of patients with polysensitization to Betulaceae pollen was considerably greater (12.88%), whereas polysensitization to Betulaceae, Poaceae and Ambrosia pollen was recorded in the highest proportion of patients (26.23%). According to age, the highest and lowest rate of allergy was recorded in the 31-50 and >51 age groups, respectively (46.22% vs 23.12%). Female predominance was observed across all age groups. The patients with monosensitization to birch pollen had the most severe symptoms in April. In the patients with poylsensitization to alder, hazel and birch pollen who developed cross-reaction, initial symptoms occurred as early as February, with abrupt exacerbation in March and April. The most severe condition was observed in the patients allergic to birch, hazel, alder, grass and ragweed pollen, with symptoms present throughout the year and exacerbation in spring and late summer months. PMID:17655183

  10. Source areas and long-range transport of pollen from continental land to Tenerife (Canary Islands)

    NASA Astrophysics Data System (ADS)

    Izquierdo, Rebeca; Belmonte, Jordina; Avila, Anna; Alarcón, Marta; Cuevas, Emilio; Alonso-Pérez, Silvia

    2011-01-01

    The Canary Islands, due to their geographical position, constitute an adequate site for the study of long-range pollen transport from the surrounding land masses. In this study, we analyzed airborne pollen counts at two sites: Santa Cruz de Tenerife (SCO), at sea level corresponding to the marine boundary layer (MBL), and Izaña at 2,367 m.a.s.l. corresponding to the free troposphere (FT), for the years 2006 and 2007. We used three approaches to describe pollen transport: (1) a classification of provenances with an ANOVA test to describe pollen count differences between sectors; (2) a study of special events of high pollen concentrations, taking into consideration the corresponding meteorological synoptic pattern responsible for transport and back trajectories; and (3) a source-receptor model applied to a selection of the pollen taxa to show pollen source areas. Our results indicate several extra-regional pollen transport episodes to Tenerife. The main provenances were: (1) the Mediterranean region, especially the southern Iberian Peninsula and Morocco, through the trade winds in the MBL. These episodes were characterized by the presence of pollen from trees ( Casuarina, Olea, Quercus perennial and deciduous types) mixed with pollen from herbs ( Artemisia, Chenopodiaceae/Amaranthaceae and Poaceae wild type). (2) The Saharan sector, through transport at the MBL level carrying pollen principally from herbs (Chenopodiaceae-Amaranthaceae, Cyperaceae and Poaceae wild type) and, in one case, Casuarina pollen, uplifted to the free troposphere. And (3) the Sahel, characterized by low pollen concentrations of Arecaceae, Chenopodiaceae-Amaranthaceae, Cyperaceae and Poaceae wild type in sporadic episodes. This research shows that sporadic events of long-range pollen transport need to be taken into consideration in Tenerife as possible responsible agents in respiratory allergy episodes. In particular, it is estimated that 89-97% of annual counts of the highly allergenous Olea

  11. Release of Bet v 1 from birch pollen from 5 European countries. Results from the HIALINE study

    NASA Astrophysics Data System (ADS)

    Buters, Jeroen T. M.; Thibaudon, Michel; Smith, Matt; Kennedy, Roy; Rantio-Lehtimäki, Auli; Albertini, Roberto; Reese, Gerald; Weber, Bernhard; Galan, Carmen; Brandao, Rui; Antunes, Celia M.; Jäger, Siegfried; Berger, Uwe; Celenk, Sevcan; Grewling, Łukasz; Jackowiak, Bogdan; Sauliene, Ingrida; Weichenmeier, Ingrid; Pusch, Gudrun; Sarioglu, Hakan; Ueffing, Marius; Behrendt, Heidrun; Prank, Marje; Sofiev, Mikhail; Cecchi, Lorenzo; Hialine Working Group

    2012-08-01

    Exposure to allergens is pivotal in determining sensitization and allergic symptoms in individuals. Pollen grain counts in ambient air have traditionally been assessed to estimate airborne allergen exposure. However, the exact allergen content of ambient air is unknown. We therefore monitored atmospheric concentrations of birch pollen grains and the matched major birch pollen allergen Bet v 1 simultaneously across Europe within the EU-funded project HIALINE (Health Impacts of Airborne Allergen Information Network). Pollen count was assessed with Hirst type pollen traps at 10 l min-1 at sites in France, United Kingdom, Germany, Italy and Finland. Allergen concentrations in ambient air were sampled at 800 l min-1 with a Chemvol® high-volume cascade impactor equipped with stages PM > 10 μm, 10 μm > PM > 2.5 μm, and in Germany also 2.5 μm > PM > 0.12 μm. The major birch pollen allergen Bet v 1 was determined with an allergen specific ELISA. Bet v 1 isoform patterns were analyzed by 2D-SDS-PAGE blots and mass spectrometric identification. Basophil activation was tested in an FcɛR1-humanized rat basophil cell line passively sensitized with serum of a birch pollen symptomatic patient. Compared to 10 previous years, 2009 was a representative birch pollen season for all stations. About 90% of the allergen was found in the PM > 10 μm fraction at all stations. Bet v 1 isoforms pattern did not vary substantially neither during ripening of pollen nor between different geographical locations. The average European allergen release from birch pollen was 3.2 pg Bet v 1/pollen and did not vary much between the European countries. However, in all countries a >10-fold difference in daily allergen release per pollen was measured which could be explained by long-range transport of pollen with a deviating allergen release. Basophil activation by ambient air extracts correlated better with airborne allergen than with pollen concentration. Although Bet v 1 is a mixture of different

  12. Release of Bet v 1 from birch pollen from 5 European countries. Results from the HIALINE study

    NASA Astrophysics Data System (ADS)

    The HIALINE working Group; Buters, Jeroen T. M.; Thibaudon, Michel; Smith, Matt; Kennedy, Roy; Rantio-Lehtimäki, Auli; Albertini, Roberto; Reese, Gerald; Weber, Bernhard; Galan, Carmen; Brandao, Rui; Antunes, Celia M.; Jäger, Siegfried; Berger, Uwe; Celenk, Sevcan; Grewling, Łukasz; Jackowiak, Bogdan; Sauliene, Ingrida; Weichenmeier, Ingrid; Pusch, Gudrun; Sarioglu, Hakan; Ueffing, Marius; Behrendt, Heidrun; Prank, Marje; Sofiev, Mikhail; Cecchi, Lorenzo

    2012-08-01

    Exposure to allergens is pivotal in determining sensitization and allergic symptoms in individuals. Pollen grain counts in ambient air have traditionally been assessed to estimate airborne allergen exposure. However, the exact allergen content of ambient air is unknown. We therefore monitored atmospheric concentrations of birch pollen grains and the matched major birch pollen allergen Bet v 1 simultaneously across Europe within the EU-funded project HIALINE (Health Impacts of Airborne Allergen Information Network).Pollen count was assessed with Hirst type pollen traps at 10 l min-1 at sites in France, United Kingdom, Germany, Italy and Finland. Allergen concentrations in ambient air were sampled at 800 l min-1 with a Chemvol® high-volume cascade impactor equipped with stages PM > 10 μm, 10 μm > PM > 2.5 μm, and in Germany also 2.5 μm > PM > 0.12 μm. The major birch pollen allergen Bet v 1 was determined with an allergen specific ELISA. Bet v 1 isoform patterns were analyzed by 2D-SDS-PAGE blots and mass spectrometric identification. Basophil activation was tested in an FcɛR1-humanized rat basophil cell line passively sensitized with serum of a birch pollen symptomatic patient.Compared to 10 previous years, 2009 was a representative birch pollen season for all stations. About 90% of the allergen was found in the PM > 10 μm fraction at all stations. Bet v 1 isoforms pattern did not vary substantially neither during ripening of pollen nor between different geographical locations. The average European allergen release from birch pollen was 3.2 pg Bet v 1/pollen and did not vary much between the European countries. However, in all countries a >10-fold difference in daily allergen release per pollen was measured which could be explained by long-range transport of pollen with a deviating allergen release. Basophil activation by ambient air extracts correlated better with airborne allergen than with pollen concentration.Although Bet v 1 is a mixture of different

  13. Second generation airborne 3D imaging lidars based on photon counting

    NASA Astrophysics Data System (ADS)

    Degnan, John J.; Wells, David; Machan, Roman; Leventhal, Edward

    2007-09-01

    The first successful photon-counting airborne laser altimeter was demonstrated in 2001 under NASA's Instrument Incubator Program (IIP). This "micro-altimeter" flew at altitudes up to 22,000 ft (6.7 km) and, using single photon returns in daylight, successfully recorded high resolution images of the underlying topography including soil, low-lying vegetation, tree canopies, water surfaces, man-made structures, ocean waves, and moving vehicles. The lidar, which operated at a wavelength of 532 nm near the peak of the solar irradiance curve, was also able to see the underlying terrain through trees and thick atmospheric haze and performed shallow water bathymetry to depths of a few meters over the Atlantic Ocean and Assawoman Bay off the Virginia coast. Sigma Space Corporation has recently developed second generation systems suitable for use in a small aircraft or mini UAV. A frequency-doubled Nd:YAG microchip laser generates few microjoule, subnanosecond pulses at fire rates up to 22 kHz. A Diffractive Optical Element (DOE) breaks the transmit beam into a 10x10 array of quasi-uniform spots which are imaged by the receive optics onto individual anodes of a high efficiency 10x10 GaAsP segmented anode microchannel plate photomultiplier. Each anode is input to one channel of a 100 channel, multistop timer demonstrated to have a 100 picosecond timing (1.5 cm range) resolution and an event recovery time less than 2 nsec. The pattern and frequency of a dual wedge optical scanner, synchronized to the laser fire rate, are tailored to provide contiguous coverage of a ground scene in a single overflight.

  14. Weather elements, chemical air pollutants and airborne pollen influencing asthma emergency room visits in Szeged, Hungary: performance of two objective weather classifications

    NASA Astrophysics Data System (ADS)

    Makra, László; Puskás, János; Matyasovszky, István; Csépe, Zoltán; Lelovics, Enikő; Bálint, Beatrix; Tusnády, Gábor

    2015-09-01

    Weather classification approaches may be useful tools in modelling the occurrence of respiratory diseases. The aim of the study is to compare the performance of an objectively defined weather classification and the Spatial Synoptic Classification (SSC) in classifying emergency department (ED) visits for acute asthma depending from weather, air pollutants, and airborne pollen variables for Szeged, Hungary, for the 9-year period 1999-2007. The research is performed for three different pollen-related periods of the year and the annual data set. According to age and gender, nine patient categories, eight meteorological variables, seven chemical air pollutants, and two pollen categories were used. In general, partly dry and cold air and partly warm and humid air aggravate substantially the symptoms of asthmatics. Our major findings are consistent with this establishment. Namely, for the objectively defined weather types favourable conditions for asthma ER visits occur when an anticyclonic ridge weather situation happens with near extreme temperature and humidity parameters. Accordingly, the SSC weather types facilitate aggravating asthmatic conditions if warm or cool weather occur with high humidity in both cases. Favourable conditions for asthma attacks are confirmed in the extreme seasons when atmospheric stability contributes to enrichment of air pollutants. The total efficiency of the two classification approaches is similar in spite of the fact that the methodology for derivation of the individual types within the two classification approaches is completely different.

  15. Weather elements, chemical air pollutants and airborne pollen influencing asthma emergency room visits in Szeged, Hungary: performance of two objective weather classifications.

    PubMed

    Makra, László; Puskás, János; Matyasovszky, István; Csépe, Zoltán; Lelovics, Enikő; Bálint, Beatrix; Tusnády, Gábor

    2015-09-01

    Weather classification approaches may be useful tools in modelling the occurrence of respiratory diseases. The aim of the study is to compare the performance of an objectively defined weather classification and the Spatial Synoptic Classification (SSC) in classifying emergency department (ED) visits for acute asthma depending from weather, air pollutants, and airborne pollen variables for Szeged, Hungary, for the 9-year period 1999-2007. The research is performed for three different pollen-related periods of the year and the annual data set. According to age and gender, nine patient categories, eight meteorological variables, seven chemical air pollutants, and two pollen categories were used. In general, partly dry and cold air and partly warm and humid air aggravate substantially the symptoms of asthmatics. Our major findings are consistent with this establishment. Namely, for the objectively defined weather types favourable conditions for asthma ER visits occur when an anticyclonic ridge weather situation happens with near extreme temperature and humidity parameters. Accordingly, the SSC weather types facilitate aggravating asthmatic conditions if warm or cool weather occur with high humidity in both cases. Favourable conditions for asthma attacks are confirmed in the extreme seasons when atmospheric stability contributes to enrichment of air pollutants. The total efficiency of the two classification approaches is similar in spite of the fact that the methodology for derivation of the individual types within the two classification approaches is completely different. PMID:25504051

  16. Diurnal variations of airborne pollen concentration and the effect of ambient temperature in three sites of Mexico City.

    PubMed

    Ríos, B; Torres-Jardón, R; Ramírez-Arriaga, E; Martínez-Bernal, A; Rosas, I

    2016-05-01

    Pollen is an important cause of allergic respiratory ailments in the Mexico City Metropolitan Area (MCMA). However, very little is known if ambient air temperature correlates with the early blooming of plants observed in other urban areas around the world. A research study was conducted during the dry season of 2012-2013 at three representative sites of the MCMA with different urban characteristics with the aim to understand the relationships between the profusion and diversity of pollen against temperature and other meteorological variables and degree of urbanization. Pollen samples were collected using a Hirst-type trap sampler in the sites: Merced (highly urbanized), Iztapalapa (medium-high urbanized) and Coyoacan (moderately urbanized). Urbanization levels were determined using a composite index based on population density, proportion of surface covered by construction and asphalt, and urban heat island intensity. A set of representative pollen sampling tapes were assayed under a light microscope at magnification of ×1,000 and converted to grains per cubic meter. The most representative pollen types found in the three sites were, regardless of urbanization levels were: Fraxinus, Cupressaceae/Taxodiaceae, Casuarina, Alnus, Myrtaceae, and Pinus. Total pollen concentration was greatest in the moderately urbanized area, although earlier blooming took place at the highly urbanized zone. Total pollen concentration in the medium-high urbanized site has the lowest because the green areas in this zone of MCMA are few. In a diurnal basis, the most abundant pollen types peaked near midday or in the afternoon evening at the three sites. A Spearman test showed a positive correlation among bihourly pollen concentrations, temperature and relative humidity in all sites, but wind speed just correlated in Iztapalapa and Coyoacan. The results obtained suggest that Urban Heat Island Intensity can disturb flowering periods and pollen concentrations, largely in the highly urbanized

  17. Diurnal variations of airborne pollen concentration and the effect of ambient temperature in three sites of Mexico City

    NASA Astrophysics Data System (ADS)

    Ríos, B.; Torres-Jardón, R.; Ramírez-Arriaga, E.; Martínez-Bernal, A.; Rosas, I.

    2016-05-01

    Pollen is an important cause of allergic respiratory ailments in the Mexico City Metropolitan Area (MCMA). However, very little is known if ambient air temperature correlates with the early blooming of plants observed in other urban areas around the world. A research study was conducted during the dry season of 2012-2013 at three representative sites of the MCMA with different urban characteristics with the aim to understand the relationships between the profusion and diversity of pollen against temperature and other meteorological variables and degree of urbanization. Pollen samples were collected using a Hirst-type trap sampler in the sites: Merced (highly urbanized), Iztapalapa (medium-high urbanized) and Coyoacan (moderately urbanized). Urbanization levels were determined using a composite index based on population density, proportion of surface covered by construction and asphalt, and urban heat island intensity. A set of representative pollen sampling tapes were assayed under a light microscope at magnification of ×1,000 and converted to grains per cubic meter. The most representative pollen types found in the three sites were, regardless of urbanization levels were: Fraxinus, Cupressaceae/Taxodiaceae, Casuarina, Alnus, Myrtaceae, and Pinus. Total pollen concentration was greatest in the moderately urbanized area, although earlier blooming took place at the highly urbanized zone. Total pollen concentration in the medium-high urbanized site has the lowest because the green areas in this zone of MCMA are few. In a diurnal basis, the most abundant pollen types peaked near midday or in the afternoon evening at the three sites. A Spearman test showed a positive correlation among bihourly pollen concentrations, temperature and relative humidity in all sites, but wind speed just correlated in Iztapalapa and Coyoacan. The results obtained suggest that Urban Heat Island Intensity can disturb flowering periods and pollen concentrations, largely in the highly urbanized

  18. Aerobiological study in east-central Iberian Peninsula: pollen diversity and dynamics for major taxa.

    PubMed

    Pérez-Badia, Rosa; Rapp, Ana; Vaquero, Consolación; Fernández-González, Federico

    2011-01-01

    A study was made of airborne pollen counts in Cuenca (east-central Iberian Peninsula, Spain), using data obtained over a 3-year period (2008-2010). This is the first such study carried out in the World Heritage city of Cuenca, situated in the large region of Castilla-La Mancha. Air monitoring was performed using the sampling and analysis procedures recommended by the Spanish Aerobiology Network. Sampling commenced in mid- 2007, and provided the first recorded pollen-spectrum for the area. The greatest pollen-type diversity was recorded in spring, whilst the highest pollen counts (over 80 percent of the annual total) were observed between February and June. The lowest counts were found in September, November and December. The 10 leading taxa, in order of abundance, were: Cupressaceae, Quercus, Urticaceae, Pinus, Olea, Poaceae, Populus, Platanus, Chenopodiaceae-Amaranthaceae and Plantago. The pollen calendar was thus typically Mediterrean, and comprised the 27 pollen types reaching 10-day mean counts of over 1 grain/m(3) of air. Maximum concentration values during the day were recorded between 12:00-20:00, coinciding with the highest temperatures and lowest humidity levels. The pollen types responsible for most allergies in the city of Cuenca, ordered by the number of days on which risk levels were reached, were: Poaceae, Urticaceae, Cupressaceae, Olea, Platanus and Chenopodiaceae-Amaranthaceae. PMID:21736275

  19. Allergenic pollen and pollen allergy in Europe.

    PubMed

    D'Amato, G; Cecchi, L; Bonini, S; Nunes, C; Annesi-Maesano, I; Behrendt, H; Liccardi, G; Popov, T; van Cauwenberge, P

    2007-09-01

    The allergenic content of the atmosphere varies according to climate, geography and vegetation. Data on the presence and prevalence of allergenic airborne pollens, obtained from both aerobiological studies and allergological investigations, make it possible to design pollen calendars with the approximate flowering period of the plants in the sampling area. In this way, even though pollen production and dispersal from year to year depend on the patterns of preseason weather and on the conditions prevailing at the time of anthesis, it is usually possible to forecast the chances of encountering high atmospheric allergenic pollen concentrations in different areas. Aerobiological and allergological studies show that the pollen map of Europe is changing also as a result of cultural factors (for example, importation of plants such as birch and cypress for urban parklands), greater international travel (e.g. colonization by ragweed in France, northern Italy, Austria, Hungary etc.) and climate change. In this regard, the higher frequency of weather extremes, like thunderstorms, and increasing episodes of long range transport of allergenic pollen represent new challenges for researchers. Furthermore, in the last few years, experimental data on pollen and subpollen-particles structure, the pathogenetic role of pollen and the interaction between pollen and air pollutants, gave new insights into the mechanisms of respiratory allergic diseases. PMID:17521313

  20. Atmospheric Poaceae pollen frequencies and associations with meteorological parameters in Brisbane, Australia: a 5-year record, 1994-1999

    NASA Astrophysics Data System (ADS)

    Green, Brett James; Dettmann, Mary; Yli-Panula, Eija; Rutherford, Shannon; Simpson, Rod

    Grass pollen is an important risk factor for allergic rhinitis and asthma in Australia and is the most prevalent pollen component of the aerospora of Brisbane, accounting for 71.6% of the annual airborne pollen load. A 5-year (June 1994-May 1999) monitoring program shows the grass pollen season to occur during the summer and autumn months (December-April), however the timing of onset and intensity of the season vary from year to year. During the pollen season, Poaceae counts exceeding 30 grains m-3 were recorded on 244 days and coincided with maximum temperatures of 28.1 +/- 2.0 °C. In this study, statistical associations between atmospheric grass pollen loads and several weather parameters, including maximum temperature, minimum temperature and precipitation, were investigated. Spearman's correlation analysis demonstrated that daily grass pollen counts were positively associated (P < 0.0001) with maximum and minimum temperature during each sampling year. Precipitation, although considered a less important daily factor (P < 0.05), was observed to remove pollen grains from the atmosphere during significant periods of rainfall. This study provides the first insight into the influence of meteorological variables, in particular temperature, on atmospheric Poaceae pollen counts in Brisbane. An awareness of these associations is critical for the prevention and management of allergy and asthma for atopic individuals within this region.

  1. An intercomparison between gross α counting and gross β counting for grab-sampling determination of airborne radon progeny and thoron progeny

    NASA Astrophysics Data System (ADS)

    Papp, Z.

    2006-03-01

    The instantaneous values of the airborne activity concentrations of radon progeny and thoron progeny have been determined 34 times in a closed and windowless room in a cellar using two independent grab-sampling methods in order to compare the performance of the methods. The activity concentration of radon ( 222Rn) was also measured and it varied between 200 and 650 Bq m -3. Two samples of radon and thoron progeny were collected simultaneously from roughly the same air volume by filtering. For the first method, the isotopes were collected on membrane filter and gross α counting was applied over several successive time intervals. This method was a slightly improved version of the methods that are applied generally for this reason for decades. For the second method, the isotopes were collected on glass-fibre filter and gross β counts were registered over several time intervals. This other method was developed a few years ago and the above series of measurements was the first opportunity to make an intercomparison between it and another similar method based on α counting. Individual radon progeny and thoron progeny activity concentrations (for the isotopes 218Po, 214Pb, 214Bi and 212Pb) were evaluated by both methods. The detailed investigation of the results showed that the systematic deviation of the methods is small but significant and isotope-dependent. The weighted averages of the β/α activity concentration ratios for 218Po, 214Pb, 214Bi, EEDC 222 (Equilibrium-Equivalent Decay-product Concentration of radon progeny) and 212Pb were 0.99±0.03, 0.90±0.02, 1.03±0.02, 0.96±0.02 and 0.80±0.03, respectively. The source of the systematic deviation is probably the inaccurate knowledge of the counting efficiencies mainly in the case of the α-counting method. A significant random-type difference between the results obtained with the two methods has also been revealed. For example, the β/α ratio for EEDC 222 varied between 0.81±0.01 and 1.22±0.03, where the

  2. The weak effects of climatic change on Plantago pollen concentration: 17 years of monitoring in Northwestern Spain

    NASA Astrophysics Data System (ADS)

    González-Parrado, Zulima; Valencia-Barrera, Rosa Ma.; Vega-Maray, Ana Ma.; Fuertes-Rodríguez, Carmen Reyes; Fernández-González, Delia

    2014-09-01

    Plantago L. species are very common in nitrified areas such as roadsides and their pollen is a major cause of pollinosis in temperate regions. In this study, we sampled airborne pollen grains in the city of León (NW, Spain) from January 1995 to December 2011, by using a Burkard® 7-day-recording trap. The percentage of Plantago pollen compared to the total pollen count ranged from 11 % (1997) to 3 % (2006) in the period under study. Peak pollen concentrations were recorded in May and June. Our 17-year analysis failed to disclose significant changes in the seasonal trend of plantain pollen concentration. In addition, there were no important changes in the start dates of pollen release and the meteorological parameters analyzed did not show significant variations in their usual trends. We analyzed the influence of several meteorological parameters on Plantago pollen concentration to explain the differences in pollen concentration trends during the study. Our results show that temperature, sun hours, evaporation, and relative humidity are the meteorological parameters best correlated to the behavior of Plantago pollen grains. In general, the years with low pollen concentrations correspond to the years with less precipitation or higher temperatures. We calculated the approximate Plantago flowering dates using the cumulative sum of daily maximum temperatures and compared them with the real bloom dates. The differences obtained were 4 days in 2009, 3 days in 2010, and 1 day in 2011 considering the complete period of pollination.

  3. The weak effects of climatic change on Plantago pollen concentration: 17 years of monitoring in Northwestern Spain.

    PubMed

    González-Parrado, Zulima; Valencia-Barrera, Rosa Ma; Vega-Maray, Ana Ma; Fuertes-Rodríguez, Carmen Reyes; Fernández-González, Delia

    2014-09-01

    Plantago L. species are very common in nitrified areas such as roadsides and their pollen is a major cause of pollinosis in temperate regions. In this study, we sampled airborne pollen grains in the city of León (NW, Spain) from January 1995 to December 2011, by using a Burkard® 7-day-recording trap. The percentage of Plantago pollen compared to the total pollen count ranged from 11% (1997) to 3% (2006) in the period under study. Peak pollen concentrations were recorded in May and June. Our 17-year analysis failed to disclose significant changes in the seasonal trend of plantain pollen concentration. In addition, there were no important changes in the start dates of pollen release and the meteorological parameters analyzed did not show significant variations in their usual trends. We analyzed the influence of several meteorological parameters on Plantago pollen concentration to explain the differences in pollen concentration trends during the study. Our results show that temperature, sun hours, evaporation, and relative humidity are the meteorological parameters best correlated to the behavior of Plantago pollen grains. In general, the years with low pollen concentrations correspond to the years with less precipitation or higher temperatures. We calculated the approximate Plantago flowering dates using the cumulative sum of daily maximum temperatures and compared them with the real bloom dates. The differences obtained were 4 days in 2009, 3 days in 2010, and 1 day in 2011 considering the complete period of pollination. PMID:24337493

  4. Cluster analysis of intradiurnal holm oak pollen cycles at peri-urban and rural sampling sites in southwestern Spain

    NASA Astrophysics Data System (ADS)

    Hernández-Ceballos, M. A.; García-Mozo, H.; Galán, C.

    2015-08-01

    The impact of regional and local weather and of local topography on intradiurnal variations in airborne pollen levels was assessed by analysing bi-hourly holm oak ( Quercus ilex subsp. ballota (Desf.) Samp.) pollen counts at two sampling stations located 40 km apart, in southwestern Spain (Cordoba city and El Cabril nature reserve) over the period 2010-2011. Pollen grains were captured using Hirst-type volumetric spore traps. Analysis of regional weather conditions was based on the computation of backward trajectories using the HYSPLIT model. Sampling days were selected on the basis of phenological data; rainy days were eliminated, as were days lying outside a given range of percentiles (P95-P5). Analysis of cycles for the study period, as a whole, revealed differences between sampling sites, with peak bi-hourly pollen counts at night in Cordoba and at midday in El Cabril. Differences were also noted in the influence of surface weather conditions (temperature, relative humidity and wind). Cluster analysis of diurnal holm oak pollen cycles revealed the existence of five clusters at each sampling site. Analysis of backward trajectories highlighted specific regional air-flow patterns associated with each site. Findings indicated the contribution of both nearby and distant pollen sources to diurnal cycles. The combined use of cluster analysis and meteorological analysis proved highly suitable for charting the impact of local weather conditions on airborne pollen-count patterns. This method, and the specific tools used here, could be used not only to study diurnal variations in counts for other pollen types and in other biogeographical settings, but also in a number of other research fields involving airborne particle transport modelling, e.g. radionuclide transport in emergency preparedness exercises.

  5. Cluster analysis of intradiurnal holm oak pollen cycles at peri-urban and rural sampling sites in southwestern Spain.

    PubMed

    Hernández-Ceballos, M A; García-Mozo, H; Galán, C

    2015-08-01

    The impact of regional and local weather and of local topography on intradiurnal variations in airborne pollen levels was assessed by analysing bi-hourly holm oak (Quercus ilex subsp. ballota (Desf.) Samp.) pollen counts at two sampling stations located 40 km apart, in southwestern Spain (Cordoba city and El Cabril nature reserve) over the period 2010-2011. Pollen grains were captured using Hirst-type volumetric spore traps. Analysis of regional weather conditions was based on the computation of backward trajectories using the HYSPLIT model. Sampling days were selected on the basis of phenological data; rainy days were eliminated, as were days lying outside a given range of percentiles (P95-P5). Analysis of cycles for the study period, as a whole, revealed differences between sampling sites, with peak bi-hourly pollen counts at night in Cordoba and at midday in El Cabril. Differences were also noted in the influence of surface weather conditions (temperature, relative humidity and wind). Cluster analysis of diurnal holm oak pollen cycles revealed the existence of five clusters at each sampling site. Analysis of backward trajectories highlighted specific regional air-flow patterns associated with each site. Findings indicated the contribution of both nearby and distant pollen sources to diurnal cycles. The combined use of cluster analysis and meteorological analysis proved highly suitable for charting the impact of local weather conditions on airborne pollen-count patterns. This method, and the specific tools used here, could be used not only to study diurnal variations in counts for other pollen types and in other biogeographical settings, but also in a number of other research fields involving airborne particle transport modelling, e.g. radionuclide transport in emergency preparedness exercises. PMID:25315264

  6. Airborne microorganism monitoring: a comparison of several methods, including a new direct counting technique.

    PubMed

    Hýsek, J; Fisar, Z; Zizka, Z; Kofronova, O; Binek, B

    1991-01-01

    Samples of aerosol from the surrounding air were collected by forcing them to impact onto a solid nutrient medium, onto membrane filters, or onto microscope slides on microcover slips. The samples were cultivated or viewed in a fluorescence microscope by using a technique developed by us, or investigated by scanning electron microscopy. The amounts of microorganisms found by cultivation method, i.e. those forming microcolonies (CFU), were on average 85% lower than the amounts determined by the fluorescence technique. Cultivation of microorganisms trapped on Synpor filters of various pore size resulted in lower counts than conventional cultivation. Among bacteria, the genera Micrococcus, Bacillus and Corynebacterium predominated; the genera Neisseria, Actinomyces, Pseudomonas and others were also found. Of micromycetes, the genera Penicillium, Aspergillus and Cladosporium occurred most frequently in air. In the summer season, yeasts and other micromycetes prevailed, whereas in the winter season the bacteria counts were higher. The total counts of all components of the aeroplankton were higher in summer than in winter. Dust particles bounded mainly bacteria. PMID:1950200

  7. Pollen spectrum and risk of pollen allergy in central Spain.

    PubMed

    Perez-Badia, Rosa; Rapp, Ana; Morales, Celia; Sardinero, Santiago; Galan, Carmen; Garcia-Mozo, Herminia

    2010-01-01

    The present work analyses the airborne pollen dynamic of the atmosphere of Toledo (central Spain), a World Heritage Site and an important tourist city receiving over 2 millions of visitors every year. The airborne pollen spectrum, the annual dynamics of the most important taxa, the influence of meteorological variables and the risk of suffering pollen allergy are analysed. Results of the present work are compared to those obtained by similar studies in nearby regions. The average annual Pollen Index is 44,632 grains, where 70-90 percent is recorded during February-May. The pollen calendar includes 29 pollen types, in order of importance; Cupressaceae (23.3 percent of the total amount of pollen grains), Quercus (21.2 percent), and Poaceae and Olea (11.5 and 11.2 percent, respectively), are the main pollen producer taxa. From an allergological viewpoint, Toledo is a high-risk locality for the residents and tourist who visit the area, with a great number of days exceeding the allergy thresholds proposed by the Spanish Aerobiological Network (REA). The types triggering most allergic processes in Toledo citizens and tourists are Cupressaceae, Platanus, Olea, Poaceae, Urticaceae and Chenopodiaceae-Amaranthaceae. Allergic risk increases in 3 main periods: winter (January-March), with the main presence of the Cupressaceae type; spring, characterized by Poaceae, Olea, Platanus and Urticaceae pollen types; and, finally, late summer (August-September), characterized by Chenopodiaceae- Amaranthaceae pollen type, which are the main cause of allergies during these months. PMID:20684492

  8. Integration of Airborne Aerosol Prediction Systems and Vegetation Phenology to Track Pollen for Asthma Alerts in Public Health Decision Support Systems

    NASA Technical Reports Server (NTRS)

    Luvall, Jeffrey C.; Sprigg, William A.; Huete, Alfredo; Pejanovic, Goran; Nickovic,Slobodan; Ponce-Campos, Guillermo; Krapfl, Heide; Budge, Amy; Zelicoff, Alan; VandeWater, Peter K.; Levetin, Estelle; Crimmins, Theresa; Weltzin, Jake

    2011-01-01

    This slide presentation reviews the study that used a model to forecast pollen to assist in warning for asthma populations. Using MODIS daily reflectances to input to a model, PREAM, adapted from the Dust REgional Atmospheric Modeling (DREAM) system, a product of predicted pollen is produced. Using the pollen from Juniper the PREAM model was shown to be an assist in alerting the public of pollen bursts, and reduce the health impact on asthma populations.

  9. A mechanistic modeling system for estimating large-scale emissions and transport of pollen and co-allergens

    NASA Astrophysics Data System (ADS)

    Efstathiou, Christos; Isukapalli, Sastry; Georgopoulos, Panos

    2011-04-01

    Allergic airway diseases represent a complex health problem which can be exacerbated by the synergistic action of pollen particles and air pollutants such as ozone. Understanding human exposures to aeroallergens requires accurate estimates of the spatial distribution of airborne pollen levels as well as of various air pollutants at different times. However, currently there are no established methods for estimating allergenic pollen emissions and concentrations over large geographic areas such as the United States. A mechanistic modeling system for describing pollen emissions and transport over extensive domains has been developed by adapting components of existing regional scale air quality models and vegetation databases. First, components of the Biogenic Emissions Inventory System (BEIS) were adapted to predict pollen emission patterns. Subsequently, the transport module of the Community Multiscale Air Quality (CMAQ) modeling system was modified to incorporate description of pollen transport. The combined model, CMAQ-pollen, allows for simultaneous prediction of multiple air pollutants and pollen levels in a single model simulation, and uses consistent assumptions related to the transport of multiple chemicals and pollen species. Application case studies for evaluating the combined modeling system included the simulation of birch and ragweed pollen levels for the year 2002, during their corresponding peak pollination periods (April for birch and September for ragweed). The model simulations were driven by previously evaluated meteorological model outputs and emissions inventories for the eastern United States for the simulation period. A semi-quantitative evaluation of CMAQ-pollen was performed using tree and ragweed pollen counts in Newark, NJ for the same time periods. The peak birch pollen concentrations were predicted to occur within two days of the peak measurements, while the temporal patterns closely followed the measured profiles of overall tree pollen

  10. Transport and radiative impacts of atmospheric pollen using online, observation-based emissions

    NASA Astrophysics Data System (ADS)

    Wozniak, M. C.; Steiner, A. L.; Solmon, F.; Li, Y.

    2015-12-01

    Atmospheric pollen emitted from trees and grasses exhibits both a high temporal variability and a highly localized spatial distribution that has been difficult to quantify in the atmosphere. Pollen's radiative impact is also not quantified because it is neglected in climate modeling studies. Here we couple an online, meteorological active pollen emissions model guided by observations of airborne pollen to understand the role of pollen in the atmosphere. We use existing pollen counts from 2003-2008 across the continental U.S. in conjunction with a tree database and historical meteorological data to create an observation-based phenological model that produces accurately scaled and timed emissions. These emissions are emitted and transported within the regional climate model (RegCM4) and the direct radiative effect is calculated. Additionally, we simulate the rupture of coarse pollen grains into finer particles by adding a second size mode for pollen emissions, which contributes to the shortwave radiative forcing and also has an indirect effect on climate.

  11. Effect of repetitive mowing on common ragweed (Ambrosia artemisiifolia L.) pollen and seed production.

    PubMed

    Simard, Marie-Josée; Benoit, Diane Lyse

    2011-01-01

    Ambrosia artemisiifolia L (common ragweed) is a familiar roadside weed in southern Québec (Canada) that produces large amounts of airborne pollen responsible for multiple rhino-conjunctivitis (hay fever) cases. As roadside weeds are increasingly controlled by mowing alone, the effect of a mowing treatment on pollen production was evaluated. Ambrosia artemisiifolia plants were grown in a greenhouse at 4 densities (1, 3, 6 and 12 plants per 314 cm(2) pot) and either left intact or mowed (10 cm from the ground) when the plants reached 25 cm in height, i.e. twice during the life cycle of this annual plant. Pollen production per male inflorescence was collected in open-top bags and counted. Inflorescence mass, length, location on the plant and date of anthesis onset was noted. Above-ground plant biomass and seed production was also evaluated. Mowed plants produced less pollen per unit of inflorescence length than intact plants. Pollen production per plant was reduced by a factor of 8.84 by the double mowing treatment, while viable seed production per plant was reduced by a factor of 4.66, irrespective of density. Mowing twice has the potential to reduce airborne pollen loads but Ambrosia artemisiifolia seed banks are unlikely to be depleted by this management strategy. PMID:21736270

  12. Integration of Airborne Aerosol Prediction Systems and Vegetation Phenology to Track Pollen for Asthma Alerts in Public Health Decision Support Systems

    NASA Technical Reports Server (NTRS)

    Luvall, Jeffrey C.; Sprigg, William A.; Huete, Alfredo; Pejanovic, Goran; Nickovic, Slobodan; Krapfl, Heide; Budge, Amy; Zelicoff, Alan; VandeWater, Peter K.; Levetin, Estelle; Losleben, Mark; Weltzin, Jake

    2009-01-01

    The residual signal indicates that the pollen event may influence the seasonal signal to an extent that would allow detection, given accurate QA filtering and BRDF corrections. MODIS daily reflectances increased during the pollen season. The DREAM model (PREAM) was successfully modified for use with pollen and may provide 24-36 hour running pollen forecasts. Publicly available pollen forecasts are linked to general weather patterns and roughly-known species phenologies. These are too coarse for timely health interventions. PREAM addresses this key data gap so that targeting intervention measures can be determined temporally and geospatially. The New Mexico Department of Health (NMDOH) as part of its Environmental Public Health Tracking Network (EPHTN) would use PREAM a tool for alerting the public in advance of pollen bursts to intervene and reduce the health impact on asthma populations at risk.

  13. Integration for Airborne Dust Prediction Systems and Vegetation Phenology to Track Pollen for Asthma Alerts in Public Health Decision Support Systems

    NASA Technical Reports Server (NTRS)

    Luvall, J. C.; Sprigg, W. A.; Nickovic, S.; Huete, A.; Budge, A.; Flowers, L.

    2008-01-01

    The objective of the program is to assess the feasibility of combining a dust transport model with MODIS derived phenology to study pollen transport for integration with a public health decision support system. The use of pollen information has specifically be identified as a critical need by the New Mexico State Health department for inclusion in the Environmental Public Health Tracking (EPHT) program. Material and methods: Pollen can be transported great distances. Local observations of plan phenology may be consistent with the timing and source of pollen collected by pollen sampling instruments. The Dust REgional Atmospheric Model (DREAM) is an integrated modeling system designed to accurately describe the dust cycle in the atmosphere. The dust modules of the entire system incorporate the state of the art parameterization of all the major phases of the atmospheric dust life such as production, diffusion, advection, and removal. These modules also include effects of the particles size distribution on aerosol dispersion. The model was modified to use pollen sources instead of dust. Pollen release was estimated based on satellite-derived phenology of key plan species and vegetation communities. The MODIS surface reflectance product (MOD09) provided information on the start of the plant growing season, growth stage, and pollen release. The resulting deterministic model is useful for predicting and simulating pollen emission and downwind concentration to study details of phenology and meteorology and their dependencies. The proposed linkage in this project provided critical information on the location timing and modeled transport of pollen directly to the EPHT> This information is useful to support the centers for disease control and prevention (CDC)'s National EPHT and the state of New Mexico environmental public health decision support for asthma and allergies alerts.

  14. Development of a regional-scale pollen emission and transport modeling framework for investigating the impact of climate change on allergic airway disease.

    PubMed

    Zhang, Rui; Duhl, Tiffany; Salam, Muhammad T; House, James M; Flagan, Richard C; Avol, Edward L; Gilliland, Frank D; Guenther, Alex; Chung, Serena H; Lamb, Brian K; VanReken, Timothy M

    2013-03-01

    Exposure to bioaerosol allergens such as pollen can cause exacerbations of allergenic airway disease (AAD) in sensitive populations, and thus cause serious public health problems. Assessing these health impacts by linking the airborne pollen levels, concentrations of respirable allergenic material, and human allergenic response under current and future climate conditions is a key step toward developing preventive and adaptive actions. To that end, a regional-scale pollen emission and transport modeling framework was developed that treats allergenic pollens as non-reactive tracers within the WRF/CMAQ air-quality modeling system. The Simulator of the Timing and Magnitude of Pollen Season (STaMPS) model was used to generate a daily pollen pool that can then be emitted into the atmosphere by wind. The STaMPS is driven by species-specific meteorological (temperature and/or precipitation) threshold conditions and is designed to be flexible with respect to its representation of vegetation species and plant functional types (PFTs). The hourly pollen emission flux was parameterized by considering the pollen pool, friction velocity, and wind threshold values. The dry deposition velocity of each species of pollen was estimated based on pollen grain size and density. An evaluation of the pollen modeling framework was conducted for southern California for the period from March to June 2010. This period coincided with observations by the University of Southern California's Children's Health Study (CHS), which included O3, PM2.5, and pollen count, as well as measurements of exhaled nitric oxide in study participants. Two nesting domains with horizontal resolutions of 12 km and 4 km were constructed, and six representative allergenic pollen genera were included: birch tree, walnut tree, mulberry tree, olive tree, oak tree, and brome grasses. Under the current parameterization scheme, the modeling framework tends to underestimate walnut and peak oak pollen concentrations, and tends

  15. Development of a regional-scale pollen emission and transport modeling framework for investigating the impact of climate change on allergic airway disease

    NASA Astrophysics Data System (ADS)

    Zhang, R.; Duhl, T.; Salam, M. T.; House, J. M.; Flagan, R. C.; Avol, E. L.; Gilliland, F. D.; Guenther, A.; Chung, S. H.; Lamb, B. K.; VanReken, T. M.

    2014-03-01

    Exposure to bioaerosol allergens such as pollen can cause exacerbations of allergenic airway disease (AAD) in sensitive populations, and thus cause serious public health problems. Assessing these health impacts by linking the airborne pollen levels, concentrations of respirable allergenic material, and human allergenic response under current and future climate conditions is a key step toward developing preventive and adaptive actions. To that end, a regional-scale pollen emission and transport modeling framework was developed that treats allergenic pollens as non-reactive tracers within the coupled Weather Research and Forecasting Community Multiscale Air Quality (WRF/CMAQ) modeling system. The Simulator of the Timing and Magnitude of Pollen Season (STaMPS) model was used to generate a daily pollen pool that can then be emitted into the atmosphere by wind. The STaMPS is driven by species-specific meteorological (temperature and/or precipitation) threshold conditions and is designed to be flexible with respect to its representation of vegetation species and plant functional types (PFTs). The hourly pollen emission flux was parameterized by considering the pollen pool, friction velocity, and wind threshold values. The dry deposition velocity of each species of pollen was estimated based on pollen grain size and density. An evaluation of the pollen modeling framework was conducted for southern California (USA) for the period from March to June 2010. This period coincided with observations by the University of Southern California's Children's Health Study (CHS), which included O3, PM2.5, and pollen count, as well as measurements of exhaled nitric oxide in study participants. Two nesting domains with horizontal resolutions of 12 and 4 km were constructed, and six representative allergenic pollen genera were included: birch tree, walnut tree, mulberry tree, olive tree, oak tree, and brome grasses. Under the current parameterization scheme, the modeling framework tends to

  16. Development of a regional-scale pollen emission and transport modeling framework for investigating the impact of climate change on allergic airway disease

    NASA Astrophysics Data System (ADS)

    Zhang, R.; Duhl, T.; Salam, M. T.; House, J. M.; Flagan, R. C.; Avol, E. L.; Gilliland, F. D.; Guenther, A.; Chung, S. H.; Lamb, B. K.; VanReken, T. M.

    2013-03-01

    Exposure to bioaerosol allergens such as pollen can cause exacerbations of allergenic airway disease (AAD) in sensitive populations, and thus cause serious public health problems. Assessing these health impacts by linking the airborne pollen levels, concentrations of respirable allergenic material, and human allergenic response under current and future climate conditions is a key step toward developing preventive and adaptive actions. To that end, a regional-scale pollen emission and transport modeling framework was developed that treats allergenic pollens as non-reactive tracers within the WRF/CMAQ air-quality modeling system. The Simulator of the Timing and Magnitude of Pollen Season (STaMPS) model was used to generate a daily pollen pool that can then be emitted into the atmosphere by wind. The STaMPS is driven by species-specific meteorological (temperature and/or precipitation) threshold conditions and is designed to be flexible with respect to its representation vegetation species and plant functional types (PFTs). The hourly pollen emission flux was parameterized by considering the pollen pool, friction velocity, and wind threshold values. The dry deposition velocity of each species of pollen was estimated based on pollen grain size and density. An evaluation of the pollen modeling framework was conducted for southern California for the period from March to June 2010. This period coincided with observations by the University of Southern California's Children's Health Study (CHS), which included O3, PM2.5, and pollen count, as well as measurements of exhaled nitric oxide in study participants. Two nesting domains with horizontal resolutions of 12 km and 4 km were constructed, and six representative allergenic pollen genera were included: birch tree, walnut tree, mulberry tree, olive tree, oak tree, and brome grasses. Under the current parameterization scheme, the modeling framework tends to underestimate walnut and peak oak pollen concentrations, and tends to

  17. Development of a regional-scale pollen emission and transport modeling framework for investigating the impact of climate change on allergic airway disease

    PubMed Central

    Zhang, Rui; Duhl, Tiffany; Salam, Muhammad T.; House, James M.; Flagan, Richard C.; Avol, Edward L.; Gilliland, Frank D.; Guenther, Alex; Chung, Serena H.; Lamb, Brian K.; VanReken, Timothy M.

    2014-01-01

    Exposure to bioaerosol allergens such as pollen can cause exacerbations of allergenic airway disease (AAD) in sensitive populations, and thus cause serious public health problems. Assessing these health impacts by linking the airborne pollen levels, concentrations of respirable allergenic material, and human allergenic response under current and future climate conditions is a key step toward developing preventive and adaptive actions. To that end, a regional-scale pollen emission and transport modeling framework was developed that treats allergenic pollens as non-reactive tracers within the WRF/CMAQ air-quality modeling system. The Simulator of the Timing and Magnitude of Pollen Season (STaMPS) model was used to generate a daily pollen pool that can then be emitted into the atmosphere by wind. The STaMPS is driven by species-specific meteorological (temperature and/or precipitation) threshold conditions and is designed to be flexible with respect to its representation of vegetation species and plant functional types (PFTs). The hourly pollen emission flux was parameterized by considering the pollen pool, friction velocity, and wind threshold values. The dry deposition velocity of each species of pollen was estimated based on pollen grain size and density. An evaluation of the pollen modeling framework was conducted for southern California for the period from March to June 2010. This period coincided with observations by the University of Southern California's Children's Health Study (CHS), which included O3, PM2.5, and pollen count, as well as measurements of exhaled nitric oxide in study participants. Two nesting domains with horizontal resolutions of 12 km and 4 km were constructed, and six representative allergenic pollen genera were included: birch tree, walnut tree, mulberry tree, olive tree, oak tree, and brome grasses. Under the current parameterization scheme, the modeling framework tends to underestimate walnut and peak oak pollen concentrations, and tends

  18. Pollen Primer

    MedlinePlus

    ... air filters (HEPA) or an electrostatic air filter. Tree Pollen Trees produce pollen earliest, as soon as January in ... distributed miles away. Fewer than 100 kinds of trees cause allergies. Some common ones are catalpa, elm, ...

  19. The patterns of Corylus and Alnus pollen seasons and pollination periods in two Polish cities located in different climatic regions.

    PubMed

    Puc, Małgorzata; Kasprzyk, Idalia

    2013-01-01

    This study compares phenological observations of Corylus (hazel) and Alnus (alder) flowering with airborne pollen counts of these taxa recorded using volumetric spore traps (2009-2011). The work was carried out in the Polish cities of Szczecin and Rzeszów that are located in different climatic regions. Correlations between pollen concentrations and meteorological data were investigated using Spearman's rank correlation analysis. The timings of hazel and alder pollination and the occurrence of airborne pollen varied greatly and were significantly influenced by meteorological conditions (p < 0.05). The flowering synchronization of hazel and alder pollination in Szczecin and Rzeszów varied over the study period. Hazel and alder trees flowered notably earlier in stands located in places that were exposed to sunlight (insolated) and sheltered from the wind. On the other hand, a delay in the timing of pollination was observed in quite sunny but very windy sites. In Rzeszów, maximum hazel pollen concentrations did not coincide with the period of full pollination (defined as between 25 % hazel and alder and 75 % of flowers open). Conversely, in Szczecin, the highest hazel pollen concentrations were recorded during phenophases of the full pollination period. The period when the highest alder pollen concentrations were recorded varied between sites, with Rzeszów recording the highest concentrations at the beginning of pollination and Szczecin recording alder pollen throughout the full pollination period. Substantial amounts of hazel and alder pollen grains were recorded in the air of Rzeszów (but not Szczecin) before the onset of the respective pollen seasons. PMID:24098067

  20. Variations in Quercus sp. pollen seasons (1996-2011) in Poznań, Poland, in relation to meteorological parameters.

    PubMed

    Grewling, Lukasz; Jackowiak, Bogdan; Smith, Matt

    2014-01-01

    The aim of this study is to supply detailed information about oak (Quercus sp.) pollen seasons in Poznań, Poland, based on a 16-year aerobiological data series (1996-2011). The pollen data were collected using a volumetric spore trap of the Hirst design located in Poznań city center. The limits of the pollen seasons were calculated using the 95 % method. The influence of meteorological parameters on temporal variations in airborne pollen was examined using correlation analysis. Start and end dates of oak pollen seasons in Poznań varied markedly from year-to-year (14 and 17 days, respectively). Most of the pollen grains (around 75 % of the seasonal pollen index) were recorded within the first 2 weeks of the pollen season. The tenfold variation was observed between the least and the most intensive pollen seasons. These fluctuations were significantly related to the variation in the sum of rain during the period second fortnight of March to first fortnight of April the year before pollination (r = 0.799; p < 0.001). During the analyzing period, a significant advance in oak pollen season start dates was observed (-0.55 day/year; p = 0.021), which was linked with an increase in the mean temperature during the second half of March and first half of April (+0.2 °C; p = 0.014). Daily average oak pollen counts correlated positively with mean and maximum daily temperatures, and negatively with daily rainfall and daily mean relative humidity. PMID:24817783

  1. Ambrosia artemisiifolia L. pollen simulations over the Euro-CORDEX domain: model description and emission calibration

    NASA Astrophysics Data System (ADS)

    liu, li; Solmon, Fabien; Giorgi, Filippo; Vautard, Robert

    2014-05-01

    Ragweed Ambrosia artemisiifolia L. is a highly allergenic invasive plant. Its pollen can be transported over large distances and has been recognized as a significant cause of hayfever and asthma (D'Amato et al., 2007). In the context of the ATOPICA EU program we are studying the links between climate, land use and ecological changes on the ragweed pollen emissions and concentrations. For this purpose, we implemented a pollen emission/transport module in the RegCM4 regional climate model in collaboration with ATOPICA partners. The Abdus Salam International Centre for Theoretical Physics (ICTP) regional climate model, i.e. RegCM4 was adapted to incorporate the pollen emissions from (ORCHIDEE French) Global Land Surface Model and a pollen tracer model for describing pollen convective transport, turbulent mixing, dry and wet deposition over extensive domains, using consistent assumption regarding the transport of multiple species (Fabien et al., 2008). We performed two families of recent-past simulations on the Euro-Cordex domain (simulation for future condition is been considering). Hindcast simulations (2000~2011) were driven by the ERA-Interim re-analyses and designed to best simulate past periods airborne pollens, which were calibrated with parts of observations and verified by comparison with the additional observations. Historical simulations (1985~2004) were driven by HadGEM CMPI5 and designed to serve as a baseline for comparison with future airborne concentrations as obtained from climate and land-use scenarios. To reduce the uncertainties on the ragweed pollen emission, an assimilation-like method (Rouǐl et al., 2009) was used to calibrate release based on airborne pollen observations. The observations were divided into two groups and used for calibration and validation separately. A wide range of possible calibration coefficients were tested for each calibration station, making the bias between observations and simulations within an admissible value then

  2. Projected Carbon Dioxide to Increase Grass Pollen and Allergen Exposure Despite Higher Ozone Levels

    PubMed Central

    Albertine, Jennifer M.; Manning, William J.; DaCosta, Michelle; Stinson, Kristina A.; Muilenberg, Michael L.; Rogers, Christine A.

    2014-01-01

    One expected effect of climate change on human health is increasing allergic and asthmatic symptoms through changes in pollen biology. Allergic diseases have a large impact on human health globally, with 10–30% of the population affected by allergic rhinitis and more than 300 million affected by asthma. Pollen from grass species, which are highly allergenic and occur worldwide, elicits allergic responses in 20% of the general population and 40% of atopic individuals. Here we examine the effects of elevated levels of two greenhouse gases, carbon dioxide (CO2), a growth and reproductive stimulator of plants, and ozone (O3), a repressor, on pollen and allergen production in Timothy grass (Phleum pratense L.). We conducted a fully factorial experiment in which plants were grown at ambient and/or elevated levels of O3 and CO2, to simulate present and projected levels of both gases and their potential interactive effects. We captured and counted pollen from flowers in each treatment and assayed for concentrations of the allergen protein, Phl p 5. We found that elevated levels of CO2 increased the amount of grass pollen produced by ∼50% per flower, regardless of O3 levels. Elevated O3 significantly reduced the Phl p 5 content of the pollen but the net effect of rising pollen numbers with elevated CO2 indicate increased allergen exposure under elevated levels of both greenhouse gases. Using quantitative estimates of increased pollen production and number of flowering plants per treatment, we estimated that airborne grass pollen concentrations will increase in the future up to ∼200%. Due to the widespread existence of grasses and the particular importance of P. pratense in eliciting allergic responses, our findings provide evidence for significant impacts on human health worldwide as a result of future climate change. PMID:25372614

  3. An automatic approach to derive vegetation height using airborne photon-counting laser altimetry data, simulating NASA's future ICESat-2 mission

    NASA Astrophysics Data System (ADS)

    Moussavi, M. S.; Abdalati, W.; Scambos, T. A.

    2011-12-01

    As the ICESat-2 mission is expected to enable large-scale assessment of terrestrial biomass, there is some concern as to whether the current instrument design will meet its ecosystem science objectives. This concern originates from the use of a low energy laser and photon-counting detector in the proposed instrument configuration that over densely vegetated areas, receives a low return (very few photons) from the ground surface. Canopy height retrievals (canopy elevation minus ground elevation) may be challenging in such areas. Here, we investigate a means of deriving canopy height using low-return-level photon-counting laser altimetry data, simulating the expected return from the planned ICESat-2 ATLAS sensor. To this end, an automatic methodology is developed, based on increasing the signal-to-noise ratio using the statistics of frames of multiple shots in the along-track direction that are comparable to the ICESat-2 footprints. We present the preliminary results of the proposed algorithm that are validated against the full-rate airborne photon counting lidar data and Digital Surface Models of the study areas. With canopy height residuals ranging from 1.34 - 1.86 m, initial results indicate promising performance over forested ecosystems of canopy closure up to 75%. These results will aid in developing data processing and analysis methods for future ICESat-2 measurements in order to maximize its application to this important science objective.

  4. Statistical approach to the analysis of olive long-term pollen season trends in southern Spain.

    PubMed

    García-Mozo, H; Yaezel, L; Oteros, J; Galán, C

    2014-03-01

    Analysis of long-term airborne pollen counts makes it possible not only to chart pollen-season trends but also to track changing patterns in flowering phenology. Changes in higher plant response over a long interval are considered among the most valuable bioindicators of climate change impact. Phenological-trend models can also provide information regarding crop production and pollen-allergen emission. The interest of this information makes essential the election of the statistical analysis for time series study. We analysed trends and variations in the olive flowering season over a 30-year period (1982-2011) in southern Europe (Córdoba, Spain), focussing on: annual Pollen Index (PI); Pollen Season Start (PSS), Peak Date (PD), Pollen Season End (PSE) and Pollen Season Duration (PSD). Apart from the traditional Linear Regression analysis, a Seasonal-Trend Decomposition procedure based on Loess (STL) and an ARIMA model were performed. Linear regression results indicated a trend toward delayed PSE and earlier PSS and PD, probably influenced by the rise in temperature. These changes are provoking longer flowering periods in the study area. The use of the STL technique provided a clearer picture of phenological behaviour. Data decomposition on pollination dynamics enabled the trend toward an alternate bearing cycle to be distinguished from the influence of other stochastic fluctuations. Results pointed to show a rising trend in pollen production. With a view toward forecasting future phenological trends, ARIMA models were constructed to predict PSD, PSS and PI until 2016. Projections displayed a better goodness of fit than those derived from linear regression. Findings suggest that olive reproductive cycle is changing considerably over the last 30years due to climate change. Further conclusions are that STL improves the effectiveness of traditional linear regression in trend analysis, and ARIMA models can provide reliable trend projections for future years taking into

  5. Synoptic and meteorological characterisation of olive pollen transport in Córdoba province (south-western Spain)

    NASA Astrophysics Data System (ADS)

    Hernández-Ceballos, Miguel A.; García-Mozo, Hermínia; Adame, José Antonio; Domínguez-Vilches, Eugenio; de La Morena, Benito A.; Bolívar, Juan Pedro; Galán, Carmen

    2011-01-01

    The main goal of the present study was to provide a detailed analysis of olive pollen transport dynamics in the province of Córdoba (south-western Spain) by applying back-trajectory analysis. Pollen data from 2006 and 2007 were analysed at four monitoring sites: Córdoba city in the centre of the province, Baena and Priego de Córdoba located in the south, and El Cabril reserve (Hornachuelos Natural Park) in the north. Particular attention was paid to nine episodes of high pollen counts. Synoptic surface maps were used, and kinematic back-trajectories (3D) were computed using the hybrid single particle Lagrangian integrated trajectory model (HYSPLIT) at 500 m above ground level, run with a time-step of 6 h over a period of 36 h. Findings were analysed in conjunction with daily and bi-hourly airborne pollen data, field phenological data and hourly surface meteorological data recorded at nearby stations: temperature, relative humidity, rainfall, wind direction and wind speed. The results identified two pollen source areas over the Córdoba province, the largest one located in the south, affecting Baena, Priego de Córdoba and Córdoba city, and one smaller located in the west, which determines mainly the pollen cycle over the north of the province, El Cabril. In addition, two air mass circulations were found, one coming from the south and crossing the main olive pollen sources very close to the surface and being frequently associated with higher pollen counts, and the other coming from the west and, in the episodes investigated, influencing mainly the north of the province.

  6. Jigsaw phase III: a miniaturized airborne 3-D imaging laser radar with photon-counting sensitivity for foliage penetration

    NASA Astrophysics Data System (ADS)

    Vaidyanathan, Mohan; Blask, Steven; Higgins, Thomas; Clifton, William; Davidsohn, Daniel; Carson, Ryan; Reynolds, Van; Pfannenstiel, Joanne; Cannata, Richard; Marino, Richard; Drover, John; Hatch, Robert; Schue, David; Freehart, Robert; Rowe, Greg; Mooney, James; Hart, Carl; Stanley, Byron; McLaughlin, Joseph; Lee, Eui-In; Berenholtz, Jack; Aull, Brian; Zayhowski, John; Vasile, Alex; Ramaswami, Prem; Ingersoll, Kevin; Amoruso, Thomas; Khan, Imran; Davis, William; Heinrichs, Richard

    2007-04-01

    Jigsaw three-dimensional (3D) imaging laser radar is a compact, light-weight system for imaging highly obscured targets through dense foliage semi-autonomously from an unmanned aircraft. The Jigsaw system uses a gimbaled sensor operating in a spot light mode to laser illuminate a cued target, and autonomously capture and produce the 3D image of hidden targets under trees at high 3D voxel resolution. With our MIT Lincoln Laboratory team members, the sensor system has been integrated into a geo-referenced 12-inch gimbal, and used in airborne data collections from a UH-1 manned helicopter, which served as a surrogate platform for the purpose of data collection and system validation. In this paper, we discuss the results from the ground integration and testing of the system, and the results from UH-1 flight data collections. We also discuss the performance results of the system obtained using ladar calibration targets.

  7. A new approach used to explore associations of current Ambrosia pollen levels with current and past meteorological elements.

    PubMed

    Matyasovszky, István; Makra, László; Csépe, Zoltán; Deák, Áron József; Pál-Molnár, Elemér; Fülöp, Andrea; Tusnády, Gábor

    2015-09-01

    The paper examines the sensitivity of daily airborne Ambrosia (ragweed) pollen levels of a current pollen season not only on daily values of meteorological variables during this season but also on the past meteorological conditions. The results obtained from a 19-year data set including daily ragweed pollen counts and ten daily meteorological variables are evaluated with special focus on the interactions between the phyto-physiological processes and the meteorological elements. Instead of a Pearson correlation measuring the strength of the linear relationship between two random variables, a generalised correlation that measures every kind of relationship between random vectors was used. These latter correlations between arrays of daily values of the ten meteorological elements and the array of daily ragweed pollen concentrations during the current pollen season were calculated. For the current pollen season, the six most important variables are two temperature variables (mean and minimum temperatures), two humidity variables (dew point depression and rainfall) and two variables characterising the mixing of the air (wind speed and the height of the planetary boundary layer). The six most important meteorological variables before the current pollen season contain four temperature variables (mean, maximum, minimum temperatures and soil temperature) and two variables that characterise large-scale weather patterns (sea level pressure and the height of the planetary boundary layer). Key periods of the past meteorological variables before the current pollen season have been identified. The importance of this kind of analysis is that a knowledge of the past meteorological conditions may contribute to a better prediction of the upcoming pollen season. PMID:25376632

  8. A new approach used to explore associations of current Ambrosia pollen levels with current and past meteorological elements

    NASA Astrophysics Data System (ADS)

    Matyasovszky, István; Makra, László; Csépe, Zoltán; Deák, Áron József; Pál-Molnár, Elemér; Fülöp, Andrea; Tusnády, Gábor

    2015-09-01

    The paper examines the sensitivity of daily airborne Ambrosia (ragweed) pollen levels of a current pollen season not only on daily values of meteorological variables during this season but also on the past meteorological conditions. The results obtained from a 19-year data set including daily ragweed pollen counts and ten daily meteorological variables are evaluated with special focus on the interactions between the phyto-physiological processes and the meteorological elements. Instead of a Pearson correlation measuring the strength of the linear relationship between two random variables, a generalised correlation that measures every kind of relationship between random vectors was used. These latter correlations between arrays of daily values of the ten meteorological elements and the array of daily ragweed pollen concentrations during the current pollen season were calculated. For the current pollen season, the six most important variables are two temperature variables (mean and minimum temperatures), two humidity variables (dew point depression and rainfall) and two variables characterising the mixing of the air (wind speed and the height of the planetary boundary layer). The six most important meteorological variables before the current pollen season contain four temperature variables (mean, maximum, minimum temperatures and soil temperature) and two variables that characterise large-scale weather patterns (sea level pressure and the height of the planetary boundary layer). Key periods of the past meteorological variables before the current pollen season have been identified. The importance of this kind of analysis is that a knowledge of the past meteorological conditions may contribute to a better prediction of the upcoming pollen season.

  9. In Vitro Pollen Viability and Pollen Germination in Cherry Laurel (Prunus laurocerasus L.)

    PubMed Central

    Sulusoglu, Melekber; Cavusoglu, Aysun

    2014-01-01

    Pollen quality is important for growers and breeders. This study was carried out to determine in vitro pollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasus L.). Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (iodine potassium iodide), were used. Pollen traits of genotypes were studied using an in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r2 = 0.0614 and r2 = 0.0015, resp.). Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media. PMID:25405230

  10. Effect of topical applications of budesonide and azelastine on nasal symptoms, eosinophil count and mediator release in atopic patients after nasal allergen challenge during the pollen season.

    PubMed

    Wang, D; Smitz, J; De Waele, M; Clement, P

    1997-10-01

    We studied the activity of a topical form of a corticosteroid (budesonide) and an antihistamine (azelastine) in the treatment of seasonal allergic rhinitis by including an assessment of mediator concentrations and the percentage of eosinophils in the nasal secretions before and after the treatment. Nasal allergen challenge (NAC) during the season was performed to mimic an acute attack of allergic rhinitis and to objectively evaluate the effect of the drugs on the early-phase reaction. The study compared in a randomized way (2 parallel groups) the effect of budesonide (Rhinocort Aqua) and azelastine (Allergodil nasal spray) in a group of 14 patients during the pollen season. The study showed that azelastine significantly reduced sneezing, total nasal resistance and increased nasal airflow even when significant increases in histamine, tryptase and leukotriene C4 (LTC4) concentrations in nasal secretions were evidenced immediately after NAC. Budesonide showed a strong (p<0.05) decrease in infiltration and activation of eosinophils, and on tryptase and LTC4 release after NAC. These effects (not for LTC4) lasted at least for 1 week after therapy. Azelastine is a powerful topical antihistamine, while budesonide appears to be a potent long-acting anti-inflammatory agent. PMID:9338613

  11. PROJECTING POLLEN ALLERGENS AND THEIR HEALTH IMPLICATIONS IN A CHANGING WORLD

    EPA Science Inventory

    This project will increase our basic understanding of the links between climatic conditions and atmospheric concentrations of pollen and pollen-derived respirable allergenic material, and impacts of airborne pollen on human health. The work will result in new parameterization...

  12. Effect of meteorological parameters on Poaceae pollen in the atmosphere of Tetouan (NW Morocco)

    NASA Astrophysics Data System (ADS)

    Aboulaich, Nadia; Achmakh, Lamiaa; Bouziane, Hassan; Trigo, M. Mar; Recio, Marta; Kadiri, Mohamed; Cabezudo, Baltasar; Riadi, Hassane; Kazzaz, Mohamed

    2013-03-01

    Poaceae pollen is one of the most prevalent aeroallergens causing allergenic reactions. The aim of this study was to characterise the grass pollen season in Tetouan during the years 2008-2010, to analyse the effect of some meteorological parameters on the incidence of the airborne Poaceae pollen, and to establish forecasting variables for daily pollen concentrations. Aerobiological sampling was undertaken over three seasons using the volumetric method. The pollen season started in April and showed the highest pollen index in May and June, when the maximum temperature ranged from 23 to 27 °C, respectively. The annual pollen score recorded varied from year to year between 2,588 and 5,404. The main pollen season lasted 114-173 days, with peak days occurring mainly in May; the highest concentration reached 308 pollen grains/m3. Air temperature was the most important meteorological parameter and correlated positively to daily pollen concentration increase. An increase in relative humidity and precipitation was usually related to a decrease in airborne pollen content. External validation of the models performed using data from 2011 showed that Poaceae pollen concentration can be highly predicted (64.2-78.6 %) from the maximum temperature, its mean concentration for the same day in other years, and its concentration recorded on the previous day. Sensitive patients suffering allergy to Poaceae pollen are at moderate to highest risk of manifesting allergic symptoms to grass pollen over 33-42 days. The results obtained provide new information on the quantitative contribution of the Poaceae pollen to the airborne pollen of Tetouan and on its temporal distribution. Airborne pollen can be surveyed and forecast in order to warn the atopic population.

  13. Ragweed pollen source inventory for France - The second largest centre of Ambrosia in Europe

    NASA Astrophysics Data System (ADS)

    Thibaudon, Michel; Šikoparija, Branko; Oliver, Gilles; Smith, Matt; Skjøth, Carsten A.

    2014-02-01

    France, in particular the Rhône-Alpes region, is one of the three main centres of ragweed (Ambrosia) in Europe. The aim of this study is to develop a gridded ragweed pollen source inventory for all of France that can be used in assessments, eradication plans and by atmospheric models for describing concentrations of airborne ragweed pollen. The inventory combines information about spatial variations in annual Ambrosia pollen counts, knowledge of ragweed ecology, detailed land cover information and a Digital Elevation Model. The ragweed inventory consists of a local infection level on a scale of 0-100% (where 100% is the highest plant abundance per area in the studied region) and a European infection level between 0% and 100% (where 100% relates to the highest identified plant abundance in Europe using the same methodology) that has been distributed onto the EMEP grid with 5 km × 5 km resolution. The results of this analysis showed that some of the highest mean annual ragweed pollen concentrations were recorded at Roussillon in the Rhône-Valley. This is reflected by the inventory, where the European infection level has been estimated to reach 67.70% of the most infected areas in Europe i.e. Kecskemét in central Hungary. The inventory shows that the Rhône Valley is the most heavily infected part of France. Central France is also infected, but northern and western parts of France are much less infected. The inventory can be entered into atmospheric transport models, in combination with other components such as a phenological model and a model for daily pollen release, in order to simulate the dispersion of ragweed pollen within France as well as potential long-distance transport from France to other European countries.

  14. Pollen clumping and wind dispersal in an invasive angiosperm.

    PubMed

    Martin, Michael D; Chamecki, Marcelo; Brush, Grace S; Meneveau, Charles; Parlange, Marc B

    2009-09-01

    Pollen dispersal is a fundamental aspect of plant reproductive biology that maintains connectivity between spatially separated populations. Pollen clumping, a characteristic feature of insect-pollinated plants, is generally assumed to be a detriment to wind pollination because clumps disperse shorter distances than do solitary pollen grains. Yet pollen clumps have been observed in dispersion studies of some widely distributed wind-pollinated species. We used Ambrosia artemisiifolia (common ragweed; Asteraceae), a successful invasive angiosperm, to investigate the effect of clumping on wind dispersal of pollen under natural conditions in a large field. Results of simultaneous measurements of clump size both in pollen shedding from male flowers and airborne pollen being dispersed in the atmosphere are combined with a transport model to show that rather than being detrimental, clumps may actually be advantageous for wind pollination. Initial clumps can pollinate the parent population, while smaller clumps that arise from breakup of larger clumps can cross-pollinate distant populations. PMID:21622356

  15. Aerobiology of Juniperus Pollen in Oklahoma, Texas, and New Mexico

    NASA Technical Reports Server (NTRS)

    Levetin, Estelle; Bunderson, Landon; VandeWater, Pete; Luvall, Jeff

    2014-01-01

    Pollen from members of the Cupressaceae are major aeroallergens in many parts of the world. In the south central and southwest United States, Juniperus pollen is the most important member of this family with J. ashei (JA) responsible for severe winter allergy symptoms in Texas and Oklahoma. In New Mexico, pollen from J. monosperma (JM) and other Juniperus species are important contributors to spring allergies, while J. pinchotii (JP) pollinates in the fall affecting sensitive individuals in west Texas, southwest Oklahoma and eastern New Mexico. Throughout this region, JA, JM, and JP occur in dense woodland populations. Generally monitoring for airborne allergens is conducted in urban areas, although the source for tree pollen may be forested areas distant from the sampling sites. Improved pollen forecasts require a better understanding of pollen production at the source. The current study was undertaken to examine the aerobiology of several Juniperus species at their source areas for the development of new pollen forecasting initiatives.

  16. Polarization Analysis of Light Scattered by Pollen Grains of Cryptomeria japonica

    NASA Astrophysics Data System (ADS)

    Iwai, Toshiaki

    2013-06-01

    Pollinosis to airborne pollen grains is a severe problem that concerns the whole world. Almost spring allergies in Japan are caused by pollen grains of Japan cedar (Cryptomeria japonica) during the period of pollination from February to May. One of the key technologies in a pollen monitoring and forecast system is a pollen sensor. The pollen grain of Japan cedar is identified by introducing the degree of polarization to the optical sensor based on the scattered intensity. The detectability and discriminability in identifying the pollen grains of Japan cedar from the polystyrene spherical particles and the Kanto loam grains are achieved up to 95 and 86%, respectively.

  17. Allergies, asthma, and pollen

    MedlinePlus

    Allergic rhinitis - pollen ... them is your first step toward feeling better. Pollen is a trigger for many people who have allergies and asthma. The types of pollens that are triggers vary from person to person ...

  18. Immunochemical quantitation of airborne short ragweed, Alternaria, antigen E, and Alt-I allergens: a two-year prospective study

    SciTech Connect

    Agarwal, M.K.; Swanson, M.C.; Reed, C.E.; Yunginger, J.W.

    1983-07-01

    We conducted a 2 yr prospective study to measure atmospheric short ragweed and Alternaria allergens by RAST inhibition analysis of eluates from filter sheets exposed in air samplers. In both years ragweed pollen and Alternaria spore counts, obtained with a rotoslide sampler, correlated significantly with immunochemically measured airborne ragweed and Alternaria allergenic activity. Airborne levels of the purified allergens AgE and Alt-I were successfully quantitated; these levels correlated closely with total airborne ragweed and Alternaria allergenic activities, respectively, and also with ragweed pollen and Alternaria spore counts. Eluates from filter sheets exposed during late summer and fall produced positive wheal-and-flare skin tests in patients with fall hay fever. In both years immunochemical measurements of allergenic activity due to airborne short ragweed correlated closely with mean symptom score indices in groups of short ragweed-sensitive individuals. Measurable levels of atmospheric ragweed allergenic activity were noted before and after the ragweed pollination season, and at these times we noted small increases in mean symptom score indices in the short ragweed-sensitive groups. Thus immunochemical analyses provide important information concerning levels of environmental allergens.

  19. Enclosed bark as a pollen trap

    USGS Publications Warehouse

    Adam, D.P.; Ferguson, C.W.; Lamarch, V.C., Jr.

    1967-01-01

    Counts were made of pollen in traps formed by enclosed bark in two remnants of bristlecone pine, Pinus aristata Engelm., from the White Mountains of east-central California. The traps, dated by tree-rings at A.D. 350 and 1300 B.C., contained a major complex of pine-sagebrush pollen and traces of other species, representing the equivalent of the present vegetation.

  20. Pollen analyses for pollination research, unacetolyzed pollen

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pollinators can significantly increase the potential yield of crops, but little is known about which pollinators pollinate various crop species. Many pollinators feed on pollen, nectar and plant secretions associated with flowers, and consequently pollen attaches to the pollinators. Identification...

  1. Role of Macrophage Migration Inhibitory Factor (MIF) in Pollen-Induced Allergic Conjunctivitis and Pollen Dermatitis in Mice

    PubMed Central

    Nagata, Yuka; Yoshihisa, Yoko; Matsunaga, Kenji; Rehman, Mati Ur; Kitaichi, Nobuyuki; Shimizu, Tadamichi

    2015-01-01

    Pollen is a clinically important airborne allergen and one of the major causes of allergic conjunctivitis. A subpopulation of patients with atopic dermatitis (AD) are also known to have exacerbated skin eruptions on the face, especially around the eyelids, after contact with pollen. This pollen-induced skin reaction is now known as pollen dermatitis. Macrophage migration inhibitory factor (MIF) is a pluripotent cytokine that plays an essential role in allergic inflammation. Recent findings suggest that MIF is involved in several allergic disorders, including AD. In this study, MIF knockout (KO), MIF transgenic (Tg) and WT littermate mice were immunized with ragweed (RW) pollen or Japanese cedar (JC) pollen and challenged via eye drops. We observed that the numbers of conjunctiva- and eyelid-infiltrating eosinophils were significantly increased in RW and JC pollen-sensitized MIF Tg compared with WT mice or MIF KO mice. The mRNA expression levels of eotaxin, interleukin (IL)-5 and IL-13 were increased in pollen-sensitized eyelid skin sites of MIF Tg mice. An in vitro analysis revealed that high eotaxin expression was induced in dermal fibroblasts by MIF combined with stimulation of IL-4 or IL-13. This eotaxin expression was inhibited by the treatment with CD74 siRNA in fibroblasts. These findings indicate that MIF can induce eosinophil accumulation in the conjunctiva and eyelid dermis exposed to pollen. Therefore, targeted inhibition of MIF might result as a new option to control pollen-induced allergic conjunctivitis and pollen dermatitis. PMID:25647395

  2. Atmospheric pollen season in Zagreb (Croatia) and its relationship with temperature and precipitation.

    PubMed

    Peternel, Renata; Srnec, Lidija; Culig, Josip; Zaninović, Ksenija; Mitić, Bozena; Vukusić, Ivan

    2004-05-01

    The number of individuals allergic to plant pollen has recently been on a constant increase, especially in large cities and industrial areas. Therefore, monitoring of airborne pollen types and concentrations during the pollen season is of the utmost medical importance. The research reported in this paper aims to determine the beginning, course and end of the pollen season for the plants in the City of Zagreb, to identify allergenic plants, and to assess the variation in airborne pollen concentration as a function of temperature and precipitation changes for the year 2002. A volumetric Hirst sampler was used for airborne pollen sampling. Qualitative and quantitative pollen analysis was performed under a light microscope (magnification x400). In the Zagreb area, 12 groups of highly allergenic plants (alder, hazel, cypress, birch, ash, hornbeam, grasses, elder, nettles, sweet chestnut, artemisia and ambrosia) were identified. Birch pollen predominated in spring, the highest concentrations being recorded in February and March. Grass pollen prevailed in May and June, and pollen of herbaceous plants of the genus Urtica (nettle) and of ambrosia in July, August and September. Air temperature was mostly higher or considerably higher than the annual average in those months, which resulted in a many days with high and very high airborne pollen concentrations. The exception was April, when these concentrations were lower because of high levels of precipitation. This also held for the first half of August and the second half of September. Pollen-sensitive individuals were at high risk from February till October because of the high airborne pollen concentrations, which only showed a transient decrease when the temperature fell or there was precipitation. PMID:14770305

  3. Atmospheric pollen season in Zagreb (Croatia) and its relationship with temperature and precipitation

    NASA Astrophysics Data System (ADS)

    Peternel, Renata; Srnec, Lidija; Čulig, Josip; Zaninović, Ksenija; Mitić, Božena; Vukušić, Ivan

    . The number of individuals allergic to plant pollen has recently been on a constant increase, especially in large cities and industrial areas. Therefore, monitoring of airborne pollen types and concentrations during the pollen season is of the utmost medical importance. The research reported in this paper aims to determine the beginning, course and end of the pollen season for the plants in the City of Zagreb, to identify allergenic plants, and to assess the variation in airborne pollen concentration as a function of temperature and precipitation changes for the year 2002. A volumetric Hirst sampler was used for airborne pollen sampling. Qualitative and quantitative pollen analysis was performed under a light microscope (magnification ×400). In the Zagreb area, 12 groups of highly allergenic plants (alder, hazel, cypress, birch, ash, hornbeam, grasses, elder, nettles, sweet chestnut, artemisia and ambrosia) were identified. Birch pollen predominated in spring, the highest concentrations being recorded in February and March. Grass pollen prevailed in May and June, and pollen of herbaceous plants of the genus Urtica (nettle) and of ambrosia in July, August and September. Air temperature was mostly higher or considerably higher than the annual average in those months, which resulted in a many days with high and very high airborne pollen concentrations. The exception was April, when these concentrations were lower because of high levels of precipitation. This also held for the first half of August and the second half of September. Pollen-sensitive individuals were at high risk from February till October because of the high airborne pollen concentrations, which only showed a transient decrease when the temperature fell or there was precipitation.

  4. Spatial and temporal modeling of daily pollen concentrations

    NASA Astrophysics Data System (ADS)

    Dellavalle, Curt T.; Triche, Elizabeth W.; Bell, Michelle L.

    2012-01-01

    Accurate assessments of pollen counts are valuable to allergy sufferers, the medical industry, and health researchers; however, monitoring stations do not exist in most areas. In addition, the degree of spatial reliability provided by the limited number of monitoring stations is poorly understood. We developed and compared spatial models to estimate pollen concentrations in locations without monitoring stations. Daily Acer, Quercus, and overall tree, grass, and weed pollen counts, in grains/m3, were obtained from 14 aeroallergen monitoring stations located in the northeastern and mid-Atlantic region of the United States from 2003 to 2006. Pollen counts were spatially interpolated using ordinary kriging. Mixed effects and generalized estimating equations incorporating daily and seasonal weather characteristics, pollen season characteristics and land-cover information were also developed to estimate daily pollen concentrations. We then compared observed values from a monitoring station to model estimates for that location. Observed counts and kriging estimates for tree pollen differed ( p = 0.04), but not when peak periods were removed ( p = 0.29). No differences between observed and kriging estimates of Acer ( p = 0.46), Quercus ( p = 0.24), grass ( p = 0.31) or weed pollen ( p = 0.29) were found. Estimates from longitudinal models also demonstrated good agreement with observed counts, except for the extremes of pollen distributions. Our results demonstrate that spatial interpolation techniques as well as regression methods incorporating both weather and land-cover characteristics can provide reliable estimates of daily pollen concentrations in areas where monitors do not exist for all but periods of extremely high pollen.

  5. Hive-stored pollen of honey bees: many lines of evidence are consistent with pollen preservation, not nutrient conversion.

    PubMed

    Anderson, Kirk E; Carroll, Mark J; Sheehan, Tim; Lanan, Michele C; Mott, Brendon M; Maes, Patrick; Corby-Harris, Vanessa

    2014-12-01

    Honey bee hives are filled with stored pollen, honey, plant resins and wax, all antimicrobial to differing degrees. Stored pollen is the nutritionally rich currency used for colony growth and consists of 40-50% simple sugars. Many studies speculate that prior to consumption by bees, stored pollen undergoes long-term nutrient conversion, becoming more nutritious 'bee bread' as microbes predigest the pollen. We quantified both structural and functional aspects associated with this hypothesis using behavioural assays, bacterial plate counts, microscopy and 454 amplicon sequencing of the 16S rRNA gene from both newly collected and hive-stored pollen. We found that bees preferentially consume fresh pollen stored for <3 days. Newly collected pollen contained few bacteria, values which decreased significantly as pollen were stored >96 h. The estimated microbe to pollen grain surface area ratio was 1:1 000 000 indicating a negligible effect of microbial metabolism on hive-stored pollen. Consistent with these findings, hive-stored pollen grains did not appear compromised according to microscopy. Based on year round 454 amplicon sequencing, bacterial communities of newly collected and hive-stored pollen did not differ, indicating the lack of an emergent microbial community co-evolved to digest stored pollen. In accord with previous culturing and 16S cloning, acid resistant and osmotolerant bacteria like Lactobacillus kunkeei were found in greatest abundance in stored pollen, consistent with the harsh character of this microenvironment. We conclude that stored pollen is not evolved for microbially mediated nutrient conversion, but is a preservative environment due primarily to added honey, nectar, bee secretions and properties of pollen itself. PMID:25319366

  6. Hive-stored pollen of honey bees: many lines of evidence are consistent with pollen preservation, not nutrient conversion

    PubMed Central

    Anderson, Kirk E; Carroll, Mark J; Sheehan, Tim; Mott, Brendon M; Maes, Patrick; Corby-Harris, Vanessa

    2014-01-01

    Honey bee hives are filled with stored pollen, honey, plant resins and wax, all antimicrobial to differing degrees. Stored pollen is the nutritionally rich currency used for colony growth and consists of 40–50% simple sugars. Many studies speculate that prior to consumption by bees, stored pollen undergoes long-term nutrient conversion, becoming more nutritious ‘bee bread’ as microbes predigest the pollen. We quantified both structural and functional aspects associated with this hypothesis using behavioural assays, bacterial plate counts, microscopy and 454 amplicon sequencing of the 16S rRNA gene from both newly collected and hive-stored pollen. We found that bees preferentially consume fresh pollen stored for <3 days. Newly collected pollen contained few bacteria, values which decreased significantly as pollen were stored >96 h. The estimated microbe to pollen grain surface area ratio was 1:1 000 000 indicating a negligible effect of microbial metabolism on hive-stored pollen. Consistent with these findings, hive-stored pollen grains did not appear compromised according to microscopy. Based on year round 454 amplicon sequencing, bacterial communities of newly collected and hive-stored pollen did not differ, indicating the lack of an emergent microbial community co-evolved to digest stored pollen. In accord with previous culturing and 16S cloning, acid resistant and osmotolerant bacteria like Lactobacillus kunkeei were found in greatest abundance in stored pollen, consistent with the harsh character of this microenvironment. We conclude that stored pollen is not evolved for microbially mediated nutrient conversion, but is a preservative environment due primarily to added honey, nectar, bee secretions and properties of pollen itself. PMID:25319366

  7. Towards a street-level pollen concentration and exposure forecast

    NASA Astrophysics Data System (ADS)

    van der Molen, Michiel; Krol, Maarten; van Vliet, Arnold; Heuvelink, Gerard

    2015-04-01

    Atmospheric pollen are an increasing source of nuisance for people in industrialised countries and are associated with significant cost of medication and sick leave. Citizen pollen warnings are often based on emission mapping based on local temperature sum approaches or on long-range atmospheric model approaches. In practise, locally observed pollen may originate from both local sources (plants in streets and gardens) and from long-range transport. We argue that making this distinction is relevant because the diurnal and spatial variation in pollen concentrations is much larger for pollen from local sources than for pollen from long-range transport due to boundary layer processes. This may have an important impact on exposure of citizens to pollen and on mitigation strategies. However, little is known about the partitioning of pollen into local and long-range origin categories. Our objective is to study how the concentrations of pollen from different sources vary temporally and spatially, and how the source region influences exposure and mitigation strategies. We built a Hay Fever Forecast system (HFF) based on WRF-chem, Allergieradar.nl, and geo-statistical downscaling techniques. HFF distinguishes between local (individual trees) and regional sources (based on tree distribution maps). We show first results on how the diurnal variation of pollen concentrations depends on source proximity. Ultimately, we will compare the model with local pollen counts, patient nuisance scores and medicine use.

  8. A combinatorial morphospace for angiosperm pollen

    NASA Astrophysics Data System (ADS)

    Mander, Luke

    2016-04-01

    The morphology of angiosperm (flowering plant) pollen is extraordinarily diverse. This diversity results from variations in the morphology of discrete anatomical components. These components include the overall shape of a pollen grain, the stratification of the exine, the number and form of any apertures, the type of dispersal unit, and the nature of any surface ornamentation. Different angiosperm pollen morphotypes reflect different combinations of these discrete components. In this talk, I ask the following question: given the anatomical components of angiosperm pollen that are known to exist in the plant kingdom, how many unique biologically plausible combinations of these components are there? I explore this question from the perspective of enumerative combinatorics using an algorithm I have written in the Python programming language. This algorithm (1) calculates the number of combinations of these components; (2) enumerates those combinations; and (3) graphically displays those combinations. The result is a combinatorial morphospace that reflects an underlying notion that the process of morphogenesis in angiosperm pollen can be thought of as an n choose k counting problem. I compare the morphology of extant and fossil angiosperm pollen grains to this morphospace, and suggest that from a combinatorial point of view angiosperm pollen is not as diverse as it could be, which may be a result of developmental constraints.

  9. Quantification of Juniperus Ashei Pollen Production for the Development of Forecasting Models

    NASA Technical Reports Server (NTRS)

    Bunderson, L. D.; Levetin, E.

    2010-01-01

    Juniperus ashei pollen is considered one of the most allergenic species of Cupressaceae in North America. Juniperus ashei is distributed throughout central Texas, Northern Mexico, the Arbuckle Mountains of south central Oklahoma, and the Ozark Mountains of northern Arkansas and southwestern Missouri. The large amount of airborne pollen that J. ashei produces affects inhabitants of cities and towns adjacent to juniper woodland areas and because juniper pollen can be transported over long distances, it affects populations that are far away. In order to create a dynamic forecast system for allergy and asthma sufferers, pollen production must be estimated. Estimation of pollen production requires the estimation of male cone production. Two locations in the Arbuckle Mountains of Oklahoma and 4 locations in the Edwards Plateau region of Texas were chosen as sampling sites. Trees were measured to determine approximate size. Male to female ratio was determined and pollen cone production was estimated using a qualitative scale from 0 to 2. Cones were counted from harvested 1/8 sections of representative trees. The representative trees were measured and approximate surface area of the tree was calculated. Using the representative tree data, the number of cones per square meter was calculated for medium production (1) and high production (2) trees. These numbers were extrapolated to calculate cone production in other trees sampled. Calibration was achieved within each location's sub-plot by counting cones on 5 branches collected from 5 sides of both high production and medium production trees. The total area sampled in each location was 0.06 hectare and total cone production varied greatly from location to location. The highest production area produced 5.8 million cones while the lowest production area produced 72,000 cones. A single representative high production tree in the Arbuckle Mountains produced 1.38 million cones. The number of trees per location was relatively

  10. Estimates of common ragweed pollen emission and dispersion over Europe using RegCM-pollen model

    NASA Astrophysics Data System (ADS)

    Liu, L.; Solmon, F.; Vautard, R.; Hamaoui-Laguel, L.; Torma, Cs. Zs.; Giorgi, F.

    2015-11-01

    Common ragweed (Ambrosia artemisiifolia L.) is a highly allergenic and invasive plant in Europe. Its pollen can be transported over large distances and has been recognized as a significant cause of hayfever and asthma (D'Amato et al., 2007; Burbach et al., 2009). To simulate production and dispersion of common ragweed pollen, we implement a pollen emission and transport module in the Regional Climate Model (RegCM) version 4 using the framework of the Community Land Model (CLM) version 4.5. In the online model environment where climate is integrated with dispersion and vegetation production, pollen emissions are calculated based on the modelling of plant distribution, pollen production, species-specific phenology, flowering probability, and flux response to meteorological conditions. A pollen tracer model is used to describe pollen advective transport, turbulent mixing, dry and wet deposition. The model is then applied and evaluated on a European domain for the period 2000-2010. To reduce the large uncertainties notably due to ragweed density distribution on pollen emission, a calibration based on airborne pollen observations is used. Resulting simulations show that the model captures the gross features of the pollen concentrations found in Europe, and reproduce reasonably both the spatial and temporal patterns of flowering season and associated pollen concentrations measured over Europe. The model can explain 68.6, 39.2, and 34.3 % of the observed variance in starting, central, and ending dates of the pollen season with associated root mean square error (RMSE) equal to 4.7, 3.9, and 7.0 days, respectively. The correlation between simulated and observed daily concentrations time series reaches 0.69. Statistical scores show that the model performs better over the central Europe source region where pollen loads are larger. From these simulations health risks associated common ragweed pollen spread are then evaluated through calculation of exposure time above health

  11. An episodic event of pollen transport of European beech

    NASA Astrophysics Data System (ADS)

    Piringer, M.; Polreich, E.; Schüler, S.; Robitschek, K.

    2010-02-01

    The meteorological impacts on pollen emission and spread in a typical Central European forest of mixed deciduous and coniferous trees are investigated. Pollen samples as well as meteorological measurements have been conducted during the flowering period of spring flowering tree species in 2009. An episodic event of pollen transport to the study area is analyzed in detail with the aid of hourly backwards trajectories. The results indicate that the experimental set-up was well designed for a thorough meteorological analysis of the pollen counts.

  12. Exposure to grass pollen--but not birch pollen--affects lung function in Swedish children.

    PubMed

    Gruzieva, O; Pershagen, G; Wickman, M; Melén, E; Hallberg, J; Bellander, T; Lõhmus, M

    2015-09-01

    Allergic response to pollen is increasing worldwide, leading to high medical and social costs. However, the effect of pollen exposure on lung function has rarely been investigated. Over 1800 children in the Swedish birth cohort BAMSE were lung-function- and IgE-tested at the age of 8 and 16 years old. Daily concentrations for 9 pollen types together with measurements for ozone, NO2 , PM10 , PM2.5 were estimated for the index day as well as up to 6 days before the testing. Exposure to grass pollen during the preceding day was associated with a reduced forced expiratory volume in 8-yr-olds; -32.4 ml; 95% CI: -50.6 to -14.2, for an increase in three pollen counts/m³. Associations appeared stronger in children sensitized to pollen allergens. As the grass species flower late in the pollen season, the allergy care routines might be weakened during this period. Therefore, allergy information may need to be updated to increase awareness among grass pollen-sensitized individuals. PMID:26011717

  13. Aerobiology, allergenicity and biochemistry of Madhuca indica Gmel. pollen.

    PubMed

    Boral, D; Roy, I; Bhattacharya, K

    1999-01-01

    An ASTIR volumetric sampler was used for one year (May 1995-April 1996) for aerobiological survey at Beharampore town, a centrally located representative part of West Bengal, to record the occurrence and frequency of airborne Madhuca pollen. The highest frequency of Madhuca pollen was recorded in April when the weather was dry with low relative humidity (RH) and moderately high temperature. Clinical test (skin prick test) showed Madhuca pollen to be one of the major causes of respiratory allergy. 30-60% (NH(4))(2)SO(4) cut fraction showed maximum positivity in skin prick test. Biochemical analysis showed that Madhuca pollen was rich in lipid and protein. SDS-PAGE was performed with the total soluble pollen protein which showed a total of 6 major protein bands, while in isolated fraction (Fr. II) a total of 7 protein bands were obtained. PMID:10607988

  14. Platanus pollen season in Andalusia (southern Spain): trends and modeling.

    PubMed

    Alcázar, Purificación; García-Mozo, Herminia; Trigo, Maria Del Mar; Ruiz, Luis; González-Minero, Francisco José; Hidalgo, Pablo; Díaz de la Guardia, Consuelo; Galán, Carmen

    2011-09-01

    Platanus is a major cause of pollen allergy in many Spanish cities. The present paper reports an analysis of Platanus pollen season throughout the Andalusia region (southern Spain), which has among the highest pollen counts and the highest incidence of Platanus-related allergies in Europe. The main aim was to analyze pollen season trends from 1992 to 2010 in Andalusia; models were also constructed to forecast the start of the season. Daily pollen counts were recorded using Hirst-type volumetric spore-traps. Pollen season start-dates were very similar at all sites, usually occurring in March. The pollen season was delayed over the study period. The Pollen-season duration and Pollen index generally increased throughout the study period. The starting date for temperature accumulation was around the 10th February, although the threshold temperatures varied by site. The regional model for Andalusia failed to provide sufficiently accurate results compared with sub-regional or local models. For modeling purposes, three sub-regions are recommended: Inland, East Coast and West Coast. PMID:21748144

  15. Hypersensitivity to common tree pollens in New York City patients.

    PubMed

    Lin, Robert Y; Clauss, Allison E; Bennett, Edward S

    2002-01-01

    Testing for tree pollen hypersensitivity typically requires the use of several tree pollens. Identifying patterns of cross-sensitivity to tree pollens could reduce the number of trees used for testing. The goal of this study was to relate reported tree pollen levels to hypersensitivity patterns. Three hundred seventy-one allergy patients were tested serologically for hypersensitivity toward prevalent tree pollens in the surrounding New York area over the years 1993-2000. Specific tree pollens that were examined included oak (Quercus alba), birch (Betula verrucosa), beech (Fagus grandifolia), poplar (Populus deltoides), maple (Acer negundo), ash (Fraxinus americana), hickory (Carya pecan), and elm (Ulmus americana). Statistical analysis of the levels of hypersensitivity was performed to identify correlations and grouping factors. Pollen levels, obtained from published annual pollen and spore reports, were characterized and related to the prevalence of hypersensitivity for the various trees. The highest prevalence of hypersensitivity (score > or = class 1) was for oak (34.3%), birch (32.9%), and maple (32.8%) tree pollens. Lower prevalences were observed for beech (29.6%), hickory (27.1%), ash (26%), elm (24.6%), and poplar (20.6%) trees. Significant correlations were observed between oak, birch, and beech radioallergosorbent test scores. Factor analysis identified two independent pollen groups with oak, birch, and beech consisting of one group and the other five tree pollens constituting the other group. Peak pollen counts clearly were highest for oak, birch, and maple trees. The peak pollen counts corresponded roughly to seropositivity prevalences for the tree pollens. When elm, poplar, and beech test scores were not used to identify patients who were allergic to tree pollens, only 1 of 106 patients with any positive tree radioallergosorbent test score was missed. It is concluded that in the New York City area, hypersensitivity to tree pollens most often is

  16. Intradiurnal variations of allergenic tree pollen in the atmosphere of Toledo (central Spain).

    PubMed

    Pérez-Badia, Rosa; Vaquero, Consolación; Sardinero, Santiago; Galán, Carmen; García-Mozo, Herminia

    2010-01-01

    To study the impact of inhaling airborne pollen on health, it is important to know not only their average daily concentrations but also the intradiurnal behaviour of these biological particles. This study reports the bi-hourly distribution of the arboreal airborne pollen types more abundant in the atmosphere of Toledo (central Spain), many of them triggering important allergic processes in Toledo citizens and tourist visitors. Knowledge of bi-hourly pattern atmospheric variation pollen may help pollinosis patients to adopt preventive measures and plan their outdoor activities accordingly. Intradiurnal variation has been studied for the arboreal pollen types: Cupressaceae, Fraxinus, Olea, Platanus, Populus, Quercus and Ulmus, during the period 2005-2008. The main hourly pollen concentrations were observed during sunlight hours and the maximum pollen values obtained at midday and in the afternoon, except for pollen types Quercus and Platanus, whose maximum pollen concentrations were obtained during the night. The statistical analyses performed to compare pollen concentration and main hourly meteorological variables proved to be significant for most of the taxa. The results show a significant and positive effect of temperature, solar radiation and wind speed on the daily variability undergone by atmospheric pollen. Relative humidity influenced in a negative way on the intradiurnal variation of pollen in the atmosphere of Toledo. PMID:21186770

  17. Modeling olive pollen intensity in the Mediterranean region through analysis of emission sources.

    PubMed

    Rojo, J; Orlandi, F; Pérez-Badia, R; Aguilera, F; Ben Dhiab, A; Bouziane, H; Díaz de la Guardia, C; Galán, C; Gutiérrez-Bustillo, A M; Moreno-Grau, S; Msallem, M; Trigo, M M; Fornaciari, M

    2016-05-01

    Aerobiological monitoring of Olea europaea L. is of great interest in the Mediterranean basin because olive pollen is one of the most represented pollen types of the airborne spectrum for the Mediterranean region, and olive pollen is considered one of the major cause of pollinosis in this region. The main aim of this study was to develop an airborne-pollen map based on the Pollen Index across a 4-year period (2008-2011), to provide a continuous geographic map for pollen intensity that will have practical applications from the agronomical and allergological points of view. For this purpose, the main predictor variable was an index based on the distribution and abundance of potential sources of pollen emission, including intrinsic information about the general atmospheric patterns of pollen dispersal. In addition, meteorological variables were included in the modeling, together with spatial interpolation, to allow the definition of a spatial model of the Pollen Index from the main olive cultivation areas in the Mediterranean region. The results show marked differences with respect to the dispersal patterns associated to the altitudinal gradient. The findings indicate that areas located at an altitude above 300ma.s.l. receive greater amounts of olive pollen from shorter-distance pollen sources (maximum influence, 27km) with respect to areas lower than 300ma.s.l. (maximum influence, 59km). PMID:26874763

  18. Pollen (quick guide)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    What is pollen, and is it haploid or diploid? Pollen is a crucial stage of the plant life cycle — without pollen there will be no seed. When someone says “Think of a plant,” the plant you think of (whether it’s a tree, a tomato plant, or a geranium) is a sporophyte. Most land plants are sporophytes...

  19. Modeling pollen time series using seasonal-trend decomposition procedure based on LOESS smoothing

    NASA Astrophysics Data System (ADS)

    Rojo, Jesús; Rivero, Rosario; Romero-Morte, Jorge; Fernández-González, Federico; Pérez-Badia, Rosa

    2016-08-01

    Analysis of airborne pollen concentrations provides valuable information on plant phenology and is thus a useful tool in agriculture—for predicting harvests in crops such as the olive and for deciding when to apply phytosanitary treatments—as well as in medicine and the environmental sciences. Variations in airborne pollen concentrations, moreover, are indicators of changing plant life cycles. By modeling pollen time series, we can not only identify the variables influencing pollen levels but also predict future pollen concentrations. In this study, airborne pollen time series were modeled using a seasonal-trend decomposition procedure based on LOcally wEighted Scatterplot Smoothing (LOESS) smoothing (STL). The data series—daily Poaceae pollen concentrations over the period 2006-2014—was broken up into seasonal and residual (stochastic) components. The seasonal component was compared with data on Poaceae flowering phenology obtained by field sampling. Residuals were fitted to a model generated from daily temperature and rainfall values, and daily pollen concentrations, using partial least squares regression (PLSR). This method was then applied to predict daily pollen concentrations for 2014 (independent validation data) using results for the seasonal component of the time series and estimates of the residual component for the period 2006-2013. Correlation between predicted and observed values was r = 0.79 (correlation coefficient) for the pre-peak period (i.e., the period prior to the peak pollen concentration) and r = 0.63 for the post-peak period. Separate analysis of each of the components of the pollen data series enables the sources of variability to be identified more accurately than by analysis of the original non-decomposed data series, and for this reason, this procedure has proved to be a suitable technique for analyzing the main environmental factors influencing airborne pollen concentrations.

  20. Are the birch trees in Southern England a source of Betula pollen for North London?

    NASA Astrophysics Data System (ADS)

    Skjøth, C. A.; Smith, M.; Brandt, J.; Emberlin, J.

    2009-01-01

    Birch pollen is highly allergenic. Knowledge of daily variations, atmospheric transport and source areas of birch pollen is important for exposure studies and for warnings to the public, especially for large cities such as London. Our results show that broad-leaved forests with high birch tree densities are located to the south and west of London. Bi-hourly Betula pollen concentrations for all the days included in the study, and for all available days with high birch pollen counts (daily average birch pollen counts >80 grains/m3), show that, on average, there is a peak between 1400 hours and 1600 hours. Back-trajectory analysis showed that, on days with high birch pollen counts ( n = 60), 80% of air masses arriving at the time of peak diurnal birch pollen count approached North London from the south in a 180 degree arc from due east to due west. Detailed investigations of three Betula pollen episodes, with distinctly different diurnal patterns compared to the mean daily cycle, were used to illustrate how night-time maxima (2200-0400 hours) in Betula pollen counts could be the result of transport from distant sources or long transport times caused by slow moving air masses. We conclude that the Betula pollen recorded in North London could originate from sources found to the west and south of the city and not just trees within London itself. Possible sources outside the city include Continental Europe and the Betula trees within the broad-leaved forests of Southern England.

  1. Regional forecast model for the Olea pollen season in Extremadura (SW Spain)

    NASA Astrophysics Data System (ADS)

    Fernández-Rodríguez, Santiago; Durán-Barroso, Pablo; Silva-Palacios, Inmaculada; Tormo-Molina, Rafael; Maya-Manzano, José María; Gonzalo-Garijo, Ángela

    2016-02-01

    The olive tree (Olea europaea) is a predominantly Mediterranean anemophilous species. The pollen allergens from this tree are an important cause of allergic problems. Olea pollen may be relevant in relation to climate change, due to the fact that its flowering phenology is related to meteorological parameters. This study aims to investigate airborne Olea pollen data from a city on the SW Iberian Peninsula, to analyse the trends in these data and their relationships with meteorological parameters using time series analysis. Aerobiological sampling was conducted from 1994 to 2013 in Badajoz (SW Spain) using a 7-day Hirst-type volumetric sampler. The main Olea pollen season lasted an average of 34 days, from May 4th to June 7th. The model proposed to forecast airborne pollen concentrations, described by one equation. This expression is composed of two terms: the first term represents the resilience of the pollen concentration trend in the air according to the average concentration of the previous 10 days; the second term was obtained from considering the actual pollen concentration value, which is calculated based on the most representative meteorological variables multiplied by a fitting coefficient. Due to the allergenic characteristics of this pollen type, it should be necessary to forecast its short-term prevalence using a long record of data in a city with a Mediterranean climate. The model obtained provides a suitable level of confidence to forecast Olea airborne pollen concentration.

  2. Comprehensive cell-specific protein analysis in early and late pollen development from diploid microsporocytes to pollen tube growth.

    PubMed

    Ischebeck, Till; Valledor, Luis; Lyon, David; Gingl, Stephanie; Nagler, Matthias; Meijón, Mónica; Egelhofer, Volker; Weckwerth, Wolfram

    2014-01-01

    Pollen development in angiosperms is one of the most important processes controlling plant reproduction and thus productivity. At the same time, pollen development is highly sensitive to environmental fluctuations, including temperature, drought, and nutrition. Therefore, pollen biology is a major focus in applied studies and breeding approaches for improving plant productivity in a globally changing climate. The most accessible developmental stages of pollen are the mature pollen and the pollen tubes, and these are thus most frequently analyzed. To reveal a complete quantitative proteome map, we additionally addressed the very early stages, analyzing eight stages of tobacco pollen development: diploid microsporocytes, meiosis, tetrads, microspores, polarized microspores, bipolar pollen, desiccated pollen, and pollen tubes. A protocol for the isolation of the early stages was established. Proteins were extracted and analyzed by means of a new gel LC-MS fractionation protocol. In total, 3817 protein groups were identified. Quantitative analysis was performed based on peptide count. Exceedingly stage-specific differential protein regulation was observed during the conversion from the sporophytic to the gametophytic proteome. A map of highly specialized functionality for the different stages could be revealed from the metabolic activity and pronounced differentiation of proteasomal and ribosomal protein complex composition up to protective mechanisms such as high levels of heat shock proteins in the very early stages of development. PMID:24078888

  3. Plant pollen content in the air of Lublin (central-eastern Poland) and risk of pollen allergy.

    PubMed

    Piotrowska-Weryszko, Krystyna; Weryszko-Chmielewska, Elżbieta

    2014-01-01

    Pollen monitoring was carried out in Lublin in 2001-2012 by the volumetric method using a Hirst-type spore trap (Lanzoni VPPS 2000). Daily pollen concentrations considerably differed in the particular years. The pollen counts with the biggest variability were observed in the first half of a year when woody plants flowering. The highest annual pollen index were noted for the following taxa: Betula, Urtica, Pinaceae, Poaceae and Alnus. Betula annual total showed the greatest diversity in the study years. The number of days on which the pollen concentration exceeded the threshold values, thereby inducing allergies, was determined for the taxa producing the most allergenic pollen. The above-mentioned taxa primarily included the following: Poaceae, in the case of which the highest number of days with the risk of occurrence of pollen allergy was found (35), Betula (18), and Artemisia (10). The following taxa: Alnus (14 days), Populus (11 days), Fraxinus (10 days), and Quercus (8 days), were also characterized by a large number of days on which their pollen concentrations exceeded the threshold values. The occurrence of periods of high concentration of particular pollen types were also noted. Risk of pollen allergy appeared the earliest at the beginning of February during Alnus and Corylus blooming. High concentrations of other woody plants were recorded from the last ten days of March to about 20 May, and of herbaceous plants from the first/last half of May-beginning of October. PMID:25528903

  4. Large Eddy Simulation of Pollen Transport in the Atmospheric Boundary Layer

    NASA Astrophysics Data System (ADS)

    Chamecki, Marcelo; Meneveau, Charles; Parlange, Marc B.

    2007-11-01

    The development of genetically modified crops and questions about cross-pollination and contamination of natural plant populations enhanced the importance of understanding wind dispersion of airborne pollen. The main objective of this work is to simulate the dispersal of pollen grains in the atmospheric surface layer using large eddy simulation. Pollen concentrations are simulated by an advection-diffusion equation including gravitational settling. Of great importance is the specification of the bottom boundary conditions characterizing the pollen source over the canopy and the deposition process everywhere else. The velocity field is discretized using a pseudospectral approach. However the application of the same discretization scheme to the pollen equation generates unphysical solutions (i.e. negative concentrations). The finite-volume bounded scheme SMART is used for the pollen equation. A conservative interpolation scheme to determine the velocity field on the finite volume surfaces was developed. The implementation is validated against field experiments of point source and area field releases of pollen.

  5. Definition of main pollen season using a logistic model.

    PubMed

    Ribeiro, Helena; Cunha, Mário; Abreu, Ilda

    2007-01-01

    This paper proposes a method to unify the definition of the main pollen season based on statistical analysis. For this, an aerobiological study was carried out in Porto region (Portugal), from 2003-2005 using a 7-day Hirst-type volumetric spore trap. To define the main pollen season, a non-linear logistic regression model was fitted to the values of the accumulated sum of the daily airborne pollen concentration from several allergological species. An important feature of this method is that the main pollen season will be characterized by the model parameters calculated. These parameters are identifiable aspects of the flowering phenology, and determine not only the beginning and end of the main pollen season, but are also influenced by the meteorological conditions. The results obtained with the proposed methodology were also compared with two of the most used percentage methods. The logistic model fitted well with the sum of accumulated pollen. The explained variance was always higher than 97%, and the exponential part of the predicted curve was well adjusted to the time when higher atmospheric pollen concentration was sampled. The comparison between the different methods tested showed large divergence in the duration and end dates of the main pollen season of the studied species. PMID:18247462

  6. Modeling the dispersion of Ambrosia artemisiifolia L. pollen with the model system COSMO-ART.

    PubMed

    Zink, Katrin; Vogel, Heike; Vogel, Bernhard; Magyar, Donát; Kottmeier, Christoph

    2012-07-01

    Common ragweed (Ambrosia artemisiifolia L.) is a highly allergenic plant that is spreading throughout Europe. Ragweed pollen can be transported over large distances by the wind. Even low pollen concentrations of less than 10 pollen m(-3) can lead to health problems in sensitive persons. Therefore, forecasting the airborne concentrations of ragweed pollen is becoming more and more important for public health. The question remains whether distant pollen sources need to be considered in reliable forecasts. We used the extended numerical weather prediction system COSMO-ART to simulate the release and transport of ragweed pollen in central Europe. A pollen episode (September 12-16, 2006) in north-eastern Germany was modeled in order to find out where the pollen originated. For this purpose, several different source regions were taken into account and their individual impact on the daily mean pollen concentration and the performance of the forecast were studied with the means of a 2 × 2 contingency table and skill scores. It was found that the majority of the pollen originated in local areas, but up to 20% of the total pollen load came from distant sources in Hungary. It is concluded that long-distance transport should not be neglected when predicting pollen concentrations. PMID:21744099

  7. Modeling the dispersion of Ambrosia artemisiifolia L. pollen with the model system COSMO-ART

    NASA Astrophysics Data System (ADS)

    Zink, Katrin; Vogel, Heike; Vogel, Bernhard; Magyar, Donát; Kottmeier, Christoph

    2012-07-01

    Common ragweed ( Ambrosia artemisiifolia L.) is a highly allergenic plant that is spreading throughout Europe. Ragweed pollen can be transported over large distances by the wind. Even low pollen concentrations of less than 10 pollen m-3 can lead to health problems in sensitive persons. Therefore, forecasting the airborne concentrations of ragweed pollen is becoming more and more important for public health. The question remains whether distant pollen sources need to be considered in reliable forecasts. We used the extended numerical weather prediction system COSMO-ART to simulate the release and transport of ragweed pollen in central Europe. A pollen episode (September 12-16, 2006) in north-eastern Germany was modeled in order to find out where the pollen originated. For this purpose, several different source regions were taken into account and their individual impact on the daily mean pollen concentration and the performance of the forecast were studied with the means of a 2 × 2 contingency table and skill scores. It was found that the majority of the pollen originated in local areas, but up to 20% of the total pollen load came from distant sources in Hungary. It is concluded that long-distance transport should not be neglected when predicting pollen concentrations.

  8. A biology-driven receptor model for daily pollen allergy risk in Korea based on Weibull probability density function

    NASA Astrophysics Data System (ADS)

    Kim, Kyu Rang; Kim, Mijin; Choe, Ho-Seong; Han, Mae Ja; Lee, Hye-Rim; Oh, Jae-Won; Kim, Baek-Jo

    2016-07-01

    Pollen is an important cause of respiratory allergic reactions. As individual sanitation has improved, allergy risk has increased, and this trend is expected to continue due to climate change. Atmospheric pollen concentration is highly influenced by weather conditions. Regression analysis and modeling of the relationships between airborne pollen concentrations and weather conditions were performed to analyze and forecast pollen conditions. Traditionally, daily pollen concentration has been estimated using regression models that describe the relationships between observed pollen concentrations and weather conditions. These models were able to forecast daily concentrations at the sites of observation, but lacked broader spatial applicability beyond those sites. To overcome this limitation, an integrated modeling scheme was developed that is designed to represent the underlying processes of pollen production and distribution. A maximum potential for airborne pollen is first determined using the Weibull probability density function. Then, daily pollen concentration is estimated using multiple regression models. Daily risk grade levels are determined based on the risk criteria used in Korea. The mean percentages of agreement between the observed and estimated levels were 81.4-88.2 % and 92.5-98.5 % for oak and Japanese hop pollens, respectively. The new models estimated daily pollen risk more accurately than the original statistical models because of the newly integrated biological response curves. Although they overestimated seasonal mean concentration, they did not simulate all of the peak concentrations. This issue would be resolved by adding more variables that affect the prevalence and internal maturity of pollens.

  9. Dating Fossil Pollen: A Simulation.

    ERIC Educational Resources Information Center

    Sheridan, Philip

    1992-01-01

    Describes a hands-on simulation in which students determine the age of "fossil" pollen samples based on the pollen types present when examined microscopically. Provides instructions for the preparation of pollen slides. (MDH)

  10. Variability within the 10-Year Pollen Rain of a Seasonal Neotropical Forest and Its Implications for Paleoenvironmental and Phenological Research

    PubMed Central

    Haselhorst, Derek S.; Moreno, J. Enrique; Punyasena, Surangi W.

    2013-01-01

    Tropical paleoecologists use a combination of mud-water interface and modern pollen rain samples (local samples of airborne pollen) to interpret compositional changes within fossil pollen records. Taxonomic similarities between the composition of modern assemblages and fossil samples are the basis of reconstructing paleoclimates and paleoenvironments. Surface sediment samples reflect a time-averaged accumulation of pollen spanning several years or more. Due to experimental constraints, modern pollen rain samples are generally collected over shorter timeframes (1–3 years) and are therefore less likely to capture the full range of natural variability in pollen rain composition and abundance. This potentially biases paleoenvironmental interpretations based on modern pollen rain transfer functions. To determine the degree to which short-term environmental change affects the composition of the aerial pollen flux of Neotropical forests, we sampled ten years of the seasonal pollen rain from Barro Colorado Island, Panama and compared it to climatic and environmental data over the same ten-year span. We establish that the pollen rain effectively captured the strong seasonality and stratification of pollen flow within the forest canopy and that individual taxa had variable sensitivity to seasonal and annual changes in environmental conditions, manifested as changes in pollen productivity. We conclude that modern pollen rain samples capture the reproductive response of moist tropical plants to short-term environmental change, but that consequently, pollen rain-based calibrations need to include longer sampling periods (≥7 years) to reflect the full range of natural variability in the pollen output of a forest and simulate the time-averaging present in sediment samples. Our results also demonstrate that over the long-term, pollen traps placed in the forest understory are representative samples of the pollen output of both canopy and understory vegetation. Aerial pollen

  11. Heterogeneity in ragweed pollen exposure is determined by plant composition at small spatial scales.

    PubMed

    Katz, Daniel S W; Carey, Tiffany S

    2014-07-01

    Pollen allergies are one of the most common health problems in the United States and over 20% of Americans are sensitized to the pollen produced by common ragweed (Ambrosia artemisiifolia L.). Despite the importance of allergenic pollen to public health, no research has linked land use and plant populations to spatial heterogeneity in airborne pollen concentrations. In order to quantify these relationships and elucidate the processes which lead to pollen exposure, we surveyed ragweed stem density in Detroit (Michigan, USA) as a function of land use. We then deployed 34 pollen collectors throughout the city and recorded ragweed cover in the immediate vicinity of each pollen collector. We found that ragweed populations were highest in vacant lots, a common land cover type in Detroit. Because ragweed population density was so strongly correlated to vacant lots, for which spatially explicit data were available, we were able to investigate whether observed ragweed pollen concentrations were a function of land use at the spatial scales of 10 m and 1 km. Both relationships were significant, and the combination of these two variables predicts a large portion of airborne ragweed pollen concentrations (R(2)=0.48). These results emphasize the important role of pollen production within the urban environment and show that management of allergenic pollen producing plants must be considered at multiple spatial scales. Our findings also demonstrate that there is too much spatial heterogeneity for a pollen collector at any given site to portray the allergenic pollen load experienced by different individuals within the same city. Finally, we discuss how spatial correlations between socio-economic status, vacant lots, and ragweed could help to explain the disproportionate amount of allergies and ragweed sensitization experienced by low income and minority populations in Detroit. PMID:24742553

  12. Effects of CO₂ on Acer negundo pollen fertility, protein content, allergenic properties, and carbohydrates.

    PubMed

    Silva, M; Ribeiro, H; Abreu, I; Cruz, A; Esteves da Silva, J C G

    2015-05-01

    Atmospheric gaseous pollutants can induce qualitative and quantitative changes in airborne pollen characteristics. In this work, it was investigated the effects of carbon dioxide (CO2) on Acer negundo pollen fertility, protein content, allergenic properties, and carbohydrates. Pollen was collected directly from the anthers and in vitro exposed to three CO2 levels (500, 1000, and 3000 ppm) for 6 and 24 h in an environmental chamber. Pollen fertility was determined using viability and germination assays, total soluble protein was determined with Coomassie Protein Assay Reagent, and the antigenic and allergenic properties were investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunological techniques using patients' sera. Also, pollen fructose, sucrose, and glucose values were determined. Carbon dioxide exposure affected negatively pollen fertility, total soluble protein content, and fructose content. The patient sera revealed increased IgE reactivity to proteins of A. negundo pollen exposed to increasing levels of the pollutant. No changes were detected in the SDS-PAGE protein profiles and in sucrose and glucose levels. Our results indicate that increase in atmospheric CO2 concentrations can have a negative influence of some features of A. negundo airborne pollen that can influence the reproductive processes as well as respiratory pollen allergies in the future. PMID:25471717

  13. Allergies, asthma, and pollen

    MedlinePlus

    ... Some trees Some grasses Weeds Ragweed Watch the weather and the season The amount of pollen in the air can affect whether you or your child has hay fever and asthma symptoms. On hot, dry, windy days, more pollen is in the air. ...

  14. Pollen calendar of the city of Salamanca (Spain). Aeropalynological analysis for 1981-1982 and 1991-1992.

    PubMed

    Hernández Prieto, M; Lorente Toledano, F; Romo Cortina, A; Dávila González, I; Laffond Yges, E; Calvo Bullón, A

    1998-01-01

    (Pyrus sp., Prunus sp., etc.), Tilia sp. (Linden), Morus sp. (mulberry), Taxus baccata (yew), Papaveraceae (Papaver rhoeas etc.), Labiata (Lavandula sp.), Cannabaceae (Humulus sp. etc.), Liliaceae (Lilium sp.), Echium sp. (viper's bugloss). The most abundant taxa, detected in the highest quantities (grains/m3 air), by order of counting were as follows: holm-oak, olive, grasses, Plantago and Parietaria. With the data obtained we have established a pollen calendar for Salamanca and report the period in which each type of pollen is found along the years and the periods with the highest airborne concentrations of such pollens. The diversity and the spectrum of the pollen in the city of Salamanca correspond to the typical plant communities found on the dehesas (large ranges of grasslands) of Castile, where Salamanca is located, although the wind directions and the peculiar climatic characteristics of the area govern the peaks of maximum presence of the different taxa. As examples, in the case of olive, which is cultivated at some distance from the city, its maximum presence coincides with south-westerly winds; in the case of grasses, the peaks of maximum counts coincide with a sharp rise in mean temperature, close to or higher than 20 degrees C and between five and six weeks after rainfall equal to or greater than 5 L/m2. PMID:9885728

  15. Pollen loads and specificity of native pollinators of lowbush blueberry.

    PubMed

    Moisan-Deserres, J; Girard, M; Chagnon, M; Fournier, V

    2014-06-01

    The reproduction of lowbush blueberry (Vaccinium angustifolium Aiton) is closely tied to insect pollination, owing to self-incompatibility. Many species are known to have greater pollination efficiency than the introduced Apis mellifera L., commonly used for commercial purposes. In this study, we measured the pollen loads of several antophilous insect species, mostly Apoidea and Syrphidae, present in four lowbush blueberry fields in Lac-St-Jean, Québec. To measure pollen loads and species specificity toward V. angustifolium, we net-collected 627 specimens of pollinators, retrieved their pollen loads, identified pollen taxa, and counted pollen grains. We found that the sizes of pollen loads were highly variable among species, ranging from a few hundred to more than 118,000 pollen grains per individual. Bombus and Andrena species in particular carried large amounts of Vaccinium pollen and thus may have greater pollination efficiency. Also, two species (Andrena bradleyi Viereck and Andrena carolina Viereck) showed nearly monolectic behavior toward lowbush blueberry. Finally, we identified alternative forage plants visited by native pollinators, notably species of Acer, Rubus, Ilex mucronata, Ledum groenlandicum, and Taraxacum. Protecting these flowering plants should be part of management practices to maintain healthy pollinator communities in a lowbush blueberry agroecosystem. PMID:25026677

  16. Seasonal variation of birch and grass pollen loads and allergen release at two sites in the German Alps

    NASA Astrophysics Data System (ADS)

    Jochner, Susanne; Lüpke, Marvin; Laube, Julia; Weichenmeier, Ingrid; Pusch, Gudrun; Traidl-Hoffmann, Claudia; Schmidt-Weber, Carsten; Buters, Jeroen T. M.; Menzel, Annette

    2015-12-01

    Less vegetated mountainous areas may provide better conditions for allergy sufferers. However, atmospheric transport can result in medically relevant pollen loads in such regions. The majority of investigations has focused on the pollen load, expressed as daily averages of pollen per cubic meter of air (pollen grains/m³); however, the severity of allergic symptoms is also determined by the actual allergen content of this pollen, its pollen potency, which may differ between high and low altitudes. We analysed airborne birch and grass pollen concentrations along with allergen content (birch: Bet v 1, grass: Phl p 5) at two different altitudes (734 and 2650 m a.s.l.) in the Zugspitze region (2009-2010). Back-trajectories were calculated for the high altitude site and for specific days with abrupt increases in pollen potency. We observed several days with medically relevant pollen concentrations at the highest site. In addition, a few days with pollen were not associated with allergens and vice versa. The calculated seasonal mean allergen release per pollen grain was 1.8-3.3 pg Bet v 1 and 5.7 pg Phl p 5 in the valley and 1.1-3.7 pg Bet v 1 and 0.7-1.5 pg Phl p 5 at the high altitude site. Back-trajectories revealed that high pollen potency at the higher site was generally associated with south-westerly to south-easterly (birch), or northerly (grass) wind directions. By investigating days with sudden increases in pollen potency, however, it was difficult to draw definitive conclusions on long- or short-range transport. Our findings suggest that people allergic to pollen might suffer less at higher altitudes and further indicate that a risk assessment relying on the actual concentration of airborne pollen does not necessarily reflect the actual allergy exposure of individuals.

  17. World Allergy Organization Study on Aerobiology for Creating First Pollen and Mold Calendar With Clinical Significance in Islamabad, Pakistan; A Project of World Allergy Organization and Pakistan Allergy, Asthma & Clinical Immunology Centre of Islamabad

    PubMed Central

    2012-01-01

    Pollen and mold allergies are highly problematic in Islamabad. This study was conducted to investigate the type and concentration of airborne pollens/molds causing allergic diseases in susceptible individuals. A volumetric spore trap (Burkard) was placed at the height of 11 m and ran continuously for 3 years. Once a week, the collecting drum was prepared by affixing Melinex tape with a double sided adhesive that was coated with a thin layer of silicone grease. Every Sunday at 9:00 AM the drum was replaced by another drum and the pollen/mold spores were removed and permanently mounted on slides. Using a microscope, the trapped particles were identified and recorded as counts per cubic meter of air per hour. From these data, the pollen and mold calendars were constructed and expressed as counts per cubic meter of air per day. Skin prick tests were performed on more than 1000 patients attending the Pakistan Allergy, Asthma & Clinical Immunology Centre of Islamabad. The results indicated that there were 2 main pollen plants that contributed to seasonal allergies. These were Broussonetia papyrifera and Cannabis sativa during the March/April season and the July/September season, respectively. Although mold spores were continuously detected throughout the year, the most prominent mold was undetected mold and unconfirmed mold species similar to Stachybotrys species, which was high from July to September/October. Two additional molds contributing to allergic reactions were Pithomyces species and Cladosporium species, which were active during January and April, with the latter also being detected between October and November. These results may prove beneficial to both patients and physicians in planning a therapeutic protocol for avoidance and amelioration. PMID:23283209

  18. Immunological Interactive Effects between Pollen Grains and Their Cytoplasmic Granules on Brown Norway Rats

    PubMed Central

    2009-01-01

    Background Grass pollen is one of the most important aeroallergen vectors in Europe. Under some meteorological factors, pollen grains can release pollen cytoplasmic granules (PCGs). PCGs induce allergic responses. Several studies have shown that during a period of thunderstorms the number of patients with asthma increases because of higher airborne concentrations of PCGs. Objective The aims of the study were to assess the allergenicity of interactive effects between pollen and PCGs and to compare it with allergenicity of Timothy grass pollen and PCGs in Brown Norway rats. Methods Rats were sensitized (day 0) and challenged (day 21) with pollen grains and/or PCGs. Four groups were studied: pollen-pollen (PP), PCGs-PCGs (GG), pollen-PCGs (PG), and PCGs-pollen (GP). Blood samples, bronchoalveolar lavage fluid, and bronchial lymph node were collected at day 25. IgE and IgG1 levels in sera were assessed by enzyme-linked immunosorbent assay. Alveolar cells, protein, and cytokine concentrations were quantified in bronchoalveolar lavage fluid. T-cell proliferation, in response to pollen or granules, was performed by lymph node assay. Results Interactive effects between pollen and PCGs increased IgE and IgG1 levels when compared with those of the negative control. These increases were lower than those of the PP group but similar to the levels obtained by the GG group. Whatever was used in the sensitization and/or challenge phase, PCGs increased lymphocyte and Rantes levels compared with those of the pollen group. The interactive effects increased IL-1α and IL-1β compared with those of the PP and GG groups. Conclusions Immunologic interactive effects have been shown between pollen and PCGs. For humoral and cellular allergic responses, interactive effects between the 2 aeroallergenic sources used in this study seem to be influenced mainly by PCGs. PMID:23283149

  19. Numerical ragweed pollen forecasts using different source maps: a comparison for France

    NASA Astrophysics Data System (ADS)

    Zink, Katrin; Kaufmann, Pirmin; Petitpierre, Blaise; Broennimann, Olivier; Guisan, Antoine; Gentilini, Eros; Rotach, Mathias W.

    2016-06-01

    One of the key input parameters for numerical pollen forecasts is the distribution of pollen sources. Generally, three different methodologies exist to assemble such distribution maps: (1) plant inventories, (2) land use data in combination with annual pollen counts, and (3) ecological modeling. We have used six exemplary maps for all of these methodologies to study their applicability and usefulness in numerical pollen forecasts. The ragweed pollen season of 2012 in France has been simulated with the numerical weather prediction model COSMO-ART using each of the distribution maps in turn. The simulated pollen concentrations were statistically compared to measured values to derive a ranking of the maps with respect to their performance. Overall, approach (2) resulted in the best correspondence between observed and simulated pollen concentrations for the year 2012. It is shown that maps resulting from ecological modeling that does not include a sophisticated estimation of the plant density have a very low predictive skill. For inventory maps and the maps based on land use data and pollen counts, the results depend very much on the observational site. The use of pollen counts to calibrate the map enhances the performance of the model considerably.

  20. All-optical automatic pollen identification: Towards an operational system

    NASA Astrophysics Data System (ADS)

    Crouzy, Benoît; Stella, Michelle; Konzelmann, Thomas; Calpini, Bertrand; Clot, Bernard

    2016-09-01

    We present results from the development and validation campaign of an optical pollen monitoring method based on time-resolved scattering and fluorescence. Focus is first set on supervised learning algorithms for pollen-taxa identification and on the determination of aerosol properties (particle size and shape). The identification capability provides a basis for a pre-operational automatic pollen season monitoring performed in parallel to manual reference measurements (Hirst-type volumetric samplers). Airborne concentrations obtained from the automatic system are compatible with those from the manual method regarding total pollen and the automatic device provides real-time data reliably (one week interruption over five months). In addition, although the calibration dataset still needs to be completed, we are able to follow the grass pollen season. The high sampling from the automatic device allows to go beyond the commonly-presented daily values and we obtain statistically significant hourly concentrations. Finally, we discuss remaining challenges for obtaining an operational automatic monitoring system and how the generic validation environment developed for the present campaign could be used for further tests of automatic pollen monitoring devices.

  1. Comparison of modern pollen distribution between the northern and southern parts of the South China Sea

    NASA Astrophysics Data System (ADS)

    Luo, Chuanxiu; Chen, Muhong; Xiang, Rong; Liu, Jianguo; Zhang, Lanlan; Lu, Jun

    2015-04-01

    The authors conducted a palynological analysis based on different number of air pollen samples for the northern and southern parts of the South China Sea, respectively, in order to give a reference to reconstruct the paleoclimate of the area. (1) Fifteen air pollen samples were collected from the northern part of the South China Sea from August to September 2011, and 13 air pollen samples were collected from the southern part of the South China Sea in December 2011. The pollen types were more abundant in the north than in the south. The total pollen number and concentration in the north was 10 times more than that in the south, which may be because of the sampling season. Airborne pollen types and concentrations have a close relationship with wind direction and distance from the sampling point to the continent. (2) Seventy-four samples were collected from surface sediments in the northern part of the South China Sea in the autumn. Thirty-three samples were collected from surface sediments in the southern part of the South China Sea in the winter. Pollen concentrations in the north were nearly 10 times higher than that in the south. This is because trilete spores are transported by rivers from Hainan Island to the sea and also by the summer monsoon-forced marine current. (3) Ten air pollen samples and 10 surface sediments samples were selected for comparison. The pollen and spores in the air were mainly herbaceous and woody pollen, excluding fern spores, having seasonal pollen characteristics. Pollen in the surface sediments were mainly trilete, Pinus, and herbaceous, and may also show a combination of annual pollen characteristics.

  2. The Pannonian plain as a source of Ambrosia pollen in the Balkans.

    PubMed

    Sikoparija, B; Smith, M; Skjøth, C A; Radisić, P; Milkovska, S; Simić, S; Brandt, J

    2009-05-01

    This study aims to find likely sources of Ambrosia pollen recorded during 2007 at five pollen-monitoring sites in central Europe: Novi Sad, Ruma, Negotin and Nis (Serbia) and Skopje (Macedonia). Ambrosia plants start flowering early in the morning and so Ambrosia pollen grains recorded during the day are likely to be from a local source. Conversely, Ambrosia pollen grains recorded at night or very early in the morning may have arrived via long-range transport. Ambrosia pollen counts were analysed in an attempt to find possible sources of the pollen and to identify Ambrosia pollen episodes suitable for further investigation using back-trajectory analysis. Diurnal variations and the magnitude of Ambrosia pollen counts during the 2007 Ambrosia pollen season showed that Novi Sad and Ruma (Pannonian Plain) and to a lesser degree Negotin (Balkans) were located near to sources of Ambrosia pollen. Mean bi-hourly Ambrosia pollen concentrations peaked during the middle of the day, and concentrations at these sites were notably higher than at Nis and Skopje. Three episodes were selected for further analysis using back-trajectory analysis. Back-trajectories showed that air masses brought Ambrosia pollen from the north to Nis and, on one occasion, to Skopje (Balkans) during the night and early morning after passing to the east of Novi Sad and Ruma during the previous day. The results of this study identified the southern part of the Pannonian Plain around Novi Sad and Ruma as being a potential source region for Ambrosia pollen recorded at Nis and Skopje in the Balkans. PMID:19224251

  3. Pollen Viability and Pollen Tube Attrition in Cranberry (Vaccinium macrocarpon)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The content of mature seed in a cranberry fruit increases with stigmatic pollen load. On average, however, only two seeds result for every tetrad of pollen deposited. What then is the fate of the two remaining pollen grains fused in each tetrad? Germination in vitro revealed that most of the grains ...

  4. A Taxonomic Reduced-Space Pollen Model for Paleoclimate Reconstruction

    NASA Astrophysics Data System (ADS)

    Wahl, E. R.; Schoelzel, C.

    2010-12-01

    Paleoenvironmental reconstruction from fossil pollen often attempts to take advantage of the rich taxonomic diversity in such data. Here, a taxonomically "reduced-space" reconstruction model is explored that would be parsimonious in introducing parameters needing to be estimated within a Bayesian Hierarchical Modeling context. This work involves a refinement of the traditional pollen ratio method. This method is useful when one (or a few) dominant pollen type(s) in a region have a strong positive correlation with a climate variable of interest and another (or a few) dominant pollen type(s) have a strong negative correlation. When, e.g., counts of pollen taxa a and b (r >0) are combined with pollen types c and d (r <0) to form ratios of the form (a + b) / (a + b + c + d), an appropriate estimation form is the binomial logistic generalized linear model (GLM). The GLM can readily model this relationship in the forward form, pollen = g(climate), which is more physically realistic than inverse models often used in paleoclimate reconstruction [climate = f(pollen)]. The specification of the model is: rnum Bin(n,p), where E(r|T) = p = exp(η)/[1+exp(η)], and η = α + β(T); r is the pollen ratio formed as above, rnum is the ratio numerator, n is the ratio denominator (i.e., the sum of pollen counts), the denominator-specific count is (n - rnum), and T is the temperature at each site corresponding to a specific value of r. Ecological and empirical screening identified the model (Spruce+Birch) / (Spruce+Birch+Oak+Hickory) for use in temperate eastern N. America. α and β were estimated using both "traditional" and Bayesian GLM algorithms (in R). Although it includes only four pollen types, the ratio model yields more explained variation ( 80%) in the pollen-temperature relationship of the study region than a 64-taxon modern analog technique (MAT). Thus, the new pollen ratio method represents an information-rich, reduced space data model that can be efficiently employed in

  5. Satellite Phenology Observations Inform Peak Season of Allergenic Grass Pollen Aerobiology across Two Continents

    NASA Astrophysics Data System (ADS)

    Huete, A. R.; Devadas, R.; Davies, J.

    2015-12-01

    Pollen exposure and prevalence of allergenic diseases have increased in many parts of the world during the last 30 years, with exposure to aeroallergen grass pollen expected to intensify with climate change, raising increased concerns for allergic diseases. The primary contributing factors to higher allergenic plant species presence are thought to be climate change, land conversion, and biotic mixing of species. Conventional methods for monitoring airborne pollen are hampered by a lack of sampling sites and heavily rely on meteorology with less attention to land cover updates and monitoring of key allergenic species phenology stages. Satellite remote sensing offers an alternative method to overcome the restrictive coverage afforded by in situ pollen networks by virtue of its synoptic coverage and repeatability of measurements that enable timely updates of land cover and land use information and monitoring landscape dynamics and interactions with human activity and climate. In this study, we assessed the potential of satellite observations of urban/peri-urban environments to directly inform landscape conditions conducive to pollen emissions. We found satellite measurements of grass cover phenological evolution to be highly correlated with in situ aerobiological grass pollen concentrations in five urban centres located across two hemispheres (Australia and France). Satellite greenness data from the Moderate Resolution Imaging Spectroradiometer (MODIS) were found to be strongly synchronous with grass pollen aerobiology in both temperate grass dominated sites (France and Melbourne), as well as in Sydney, where multiple pollen peaks coincided with the presence of subtropical grasses. Employing general additive models (GAM), the satellite phenology data provided strong predictive capabilities to inform airborne pollen levels and forecast periods of grass pollen emissions at all five sites. Satellite phenology offer promising opportunities of improving public health risk

  6. Large Eddy Simulation and Field Experiments of Pollen Transport in the Atmospheric Boundary Layer

    NASA Astrophysics Data System (ADS)

    Chamecki, M.; Meneveau, C.; Parlange, M. B.; van Hout, R.

    2006-12-01

    Dispersion of airborne pollen by the wind has been a subject of interest for botanists and allergists for a long time. More recently, the development of genetically modified crops and questions about cross-pollination and subsequent contamination of natural plant populations has brought even more interest to this field. A critical question is how far from the source field pollen grains will be advected. Clearly the answer depends on the aerodynamic properties of the pollen, geometrical properties of the field, topography, local vegetation, wind conditions, atmospheric stability, etc. As a consequence, field experiments are well suited to provide some information on pollen transport mechanisms but are limited to specific field and weather conditions. Numerical simulations do not have this drawback and can be a useful tool to study pollen dispersal in a variety of configurations. It is well known that the dispersion of particles in turbulent fields is strongly affected by the large scale coherent structures. Large Eddy Simulation (LES) is a technique that allows us to study the typical distances reached by pollen grains and, at the same time, resolve the larger coherent structures present in the atmospheric boundary layer. The main objective of this work is to simulate the dispersal of pollen grains in the atmospheric surface layer using LES. Pollen concentrations are simulated by an advection-diffusion equation including gravitational settling. Of extreme importance is the specification of the bottom boundary conditions characterizing the pollen source over the canopy and the deposition process everywhere else. In both cases we make use of the theoretical profile for suspended particles derived by Kind (1992). Field experiments were performed to study the applicability of the theoretical profile to pollen grains and the results are encouraging. Airborne concentrations as well as ground deposition from the simulations are compared to experimental data to validate the

  7. Cell counting.

    PubMed

    Phelan, M C; Lawler, G

    2001-05-01

    This unit presents protocols for counting cells using either a hemacytometer or electronically using a Coulter counter. Cell counting with a hemacytometer permits effective discrimination of live from dead cells using trypan blue exclusion. In addition, the procedure is less subject to errors arising from cell clumping or size heterogeneity. Counting cells is more quickly and easily performed using an electronic counter, but live-dead discrimination is unreliable. Cell populations containing large numbers of dead cells and/or cell clumps are difficult to count accurately. In addition, electronic counting requires resetting of the instrument for cell populations of different sizes; heterogeneous populations can give rise to inaccurate counts, and resting and activated cells may require counting at separate settings. In general, electronic cell counting is best performed on fresh peripheral blood cells. PMID:18770655

  8. Climate change and its impact on birch pollen quantities and the start of the pollen season an example from Switzerland for the period 1969-2006

    NASA Astrophysics Data System (ADS)

    Frei, Thomas; Gassner, Ewald

    2008-09-01

    As published by the Intergovernmental Panel on Climate Change (IPCC) global warming is a reality and its impact is huge like the increase of extreme weather events, glacier recession, sea level rise and also effects on human health. Among them allergies to airborne pollen might increase or change in pattern due to the invasion of new allergic plants or due to different behavior of plants like earlier flowering. In this study we used the longest Swiss airborne pollen data set to examine the influence of the temperature increase on the time of flowering. In the case of Basel, where pollen data for 38 years are available, it was shown that due to a temperature increase the start of flowering in the case of birch occurred about 15 days earlier. Apart from a shift of the start of the flowering there is also a trend towards higher annual birch pollen quantities and an increase of the highest daily mean pollen concentrations. Due to global warming and because symptoms may appear earlier in the year people suffering from a pollen allergy might face a new unaccustomed situation.

  9. Evaluation of the Nutritional and Storage Quality of Meatballs Formulated with Bee Pollen

    PubMed Central

    2014-01-01

    In this study, the nutritional and storage quality of meatballs formulated with different levels (0, 1.5, 3.0, 4.5 and 6.0%) of bee pollen were investigated during storage at 41℃ for 9 d. Protein content of meatballs increased, while moisture content decreased with increased pollen. The addition of pollen improved cooking loss but decreased the redness (Hunter a value) and sensory scores. Textural parameters (hardness, springsness, gumminess, and chewiness) were affected by pollen addition and the hardness and gumminess values of meatballs decreased as the pollen content increased. While C18:0 content of meatballs slightly decreased with pollen addition, C18:2n-6c, C18:3n-3, C20:5n-3, and PUFA contents increased. The PUFA/saturated fatty acids (P/S) ratio increased from 0.05 in the control to 0.09 in meatballs with 6.0% pollen. The n-6/n-3 ratio decreased from 11.84 in the control to 3.65 in the meatballs with 6.0% pollen. The addition of pollen retarded the lipid oxidation and inhibited the bacterial growth in meatballs. The pH, redness, TBA value and total aerobic mesophilic bacteria, coliform bacteria and S. aureus counts values changed significantly during storage. The results suggest that bee pollen could be added to enhance the nutritional and storage quality of meatballs with minimal changes in composition and/or sensory properties. PMID:26761280

  10. Modelling past land use using archaeological and pollen data

    NASA Astrophysics Data System (ADS)

    Pirzamanbein, Behnaz; Lindström, johan; Poska, Anneli; Gaillard-Lemdahl, Marie-José

    2016-04-01

    Accurate maps of past land use are necessary for studying the impact of anthropogenic land-cover changes on climate and biodiversity. We develop a Bayesian hierarchical model to reconstruct the land use using Gaussian Markov random fields. The model uses two observations sets: 1) archaeological data, representing human settlements, urbanization and agricultural findings; and 2) pollen-based land estimates of the three land-cover types Coniferous forest, Broadleaved forest and Unforested/Open land. The pollen based estimates are obtained from the REVEALS model, based on pollen counts from lakes and bogs. Our developed model uses the sparse pollen-based estimations to reconstruct the spatial continuous cover of three land cover types. Using the open-land component and the archaeological data, the extent of land-use is reconstructed. The model is applied on three time periods - centred around 1900 CE, 1000 and, 4000 BCE over Sweden for which both pollen-based estimates and archaeological data are available. To estimate the model parameters and land use, a block updated Markov chain Monte Carlo (MCMC) algorithm is applied. Using the MCMC posterior samples uncertainties in land-use predictions are computed. Due to lack of good historic land use data, model results are evaluated by cross-validation. Keywords. Spatial reconstruction, Gaussian Markov random field, Fossil pollen records, Archaeological data, Human land-use, Prediction uncertainty

  11. Contribution of pollen to atmospheric ice nuclei concentrations

    NASA Astrophysics Data System (ADS)

    Hader, J. D.; Wright, T. P.; Petters, M. D.

    2014-06-01

    Recent studies have suggested that the ice-nucleating ability of some types of pollen is derived from non-proteinaceous macromolecules. These macromolecules may become dispersed by the rupturing of the pollen grain during wetting and drying cycles in the atmosphere. If true, this mechanism might prove to be a significant source of ice nuclei (IN) concentrations when pollen is present. Here we test this hypothesis by measuring ambient IN concentrations from the beginning to the end of the 2013 pollen season in Raleigh, North Carolina, USA. Air samples were collected using a swirling aerosol collector twice per week and the solutions were analysed for ice nuclei activity using a droplet freezing assay. Rainwater samples were collected at times when pollen grain number concentrations were near their maximum value and analysed with the drop-freezing assay to compare the potentially enhanced IN concentrations measured near the ground with IN concentrations found aloft. Ambient ice nuclei spectra, defined as the number of ice nuclei per volume of air as a function of temperature, are inferred from the aerosol collector solutions. No general trend was observed between ambient pollen grain counts and observed IN concentrations, suggesting that ice nuclei multiplication via pollen grain rupturing and subsequent release of macromolecules was not prevalent for the pollen types and meteorological conditions typically encountered in the southeastern US. A serendipitously sampled collection after a downpour provided evidence for a rain-induced IN burst with an observed IN concentration of approximately 30 per litre, a 30-fold increase over background concentrations at -20 °C. The onset temperature of freezing for these particles was approximately -12 °C, suggesting that the ice-nucleating particles were biological in origin.

  12. Contribution of pollen to atmospheric ice nuclei concentrations

    NASA Astrophysics Data System (ADS)

    Hader, J. D.; Wright, T. P.; Petters, M. D.

    2013-12-01

    Recent studies have suggested that the ice nucleating ability of some types of pollen is derived from non-proteinaceous macromolecules. These macromolecules may become dispersed by the rupturing of the pollen sac during wetting and drying cycles in the atmosphere. If true, this mechanism might prove to be a significant source of ice nuclei (IN) concentrations when pollen are present. Here we test this hypothesis by measuring ambient IN concentrations from the beginning to the end of the 2013 pollen season in Raleigh, North Carolina, USA. Air samples were collected using a swirling aerosol collector twice per week and the solutions were analysed for ice nuclei activity using a droplet freezing assay. Rainwater samples were collected at the peak of the pollen season and analysed with the drop freezing assay to compare the potentially enhanced IN concentrations measured near the ground with IN concentrations found aloft. Ambient ice nuclei spectra, defined as the number of ice nuclei per volume of air as a function of temperature, are inferred from the aerosol collector solutions. No general trend was observed between ambient pollen counts and observed IN concentrations, suggesting that ice nuclei multiplication via pollen sac rupturing and subsequent release of macromolecules was not prevalent for the pollen types and meteorological conditions typically encountered in the Southeastern US. A serendipitously sampled collection after a downpour provided evidence for a rain-induced IN burst with an observed IN concentration of approximately 30 per litre, a 30-fold increase over background concentrations at -20 °C. The onset temperature of freezing for these particles was approximately -12 °C, suggesting that the ice nucleating particles were biological in origin. The magnitude of the IN burst was significantly larger than previously observed, providing additional evidence to merit further investigation of a self-regulated feedback cycle between the atmosphere and

  13. Lipid biomarker analysis for the quantitative analysis of airborne microorganisms

    SciTech Connect

    Macnaughton, S.J.; Jenkins, T.L.; Cormier, M.R.

    1997-08-01

    There is an ever increasing concern regarding the presence of airborne microbial contaminants within indoor air environments. Exposure to such biocontaminants can give rise to large numbers of different health effects including infectious diseases, allergenic responses and respiratory problems, Biocontaminants typically round in indoor air environments include bacteria, fungi, algae, protozoa and dust mites. Mycotoxins, endotoxins, pollens and residues of organisms are also known to cause adverse health effects. A quantitative detection/identification technique independent of culturability that assays both culturable and non culturable biomass including endotoxin is critical in defining risks from indoor air biocontamination. Traditionally, methods employed for the monitoring of microorganism numbers in indoor air environments involve classical culture based techniques and/or direct microscopic counting. It has been repeatedly documented that viable microorganism counts only account for between 0.1-10% of the total community detectable by direct counting. The classic viable microbiologic approach doe`s not provide accurate estimates of microbial fragments or other indoor air components that can act as antigens and induce or potentiate allergic responses. Although bioaerosol samplers are designed to damage the microbes as little as possible, microbial stress has been shown to result from air sampling, aerosolization and microbial collection. Higher collection efficiency results in greater cell damage while less cell damage often results in lower collection efficiency. Filtration can collect particulates at almost 100% efficiency, but captured microorganisms may become dehydrated and damaged resulting in non-culturability, however, the lipid biomarker assays described herein do not rely on cell culture. Lipids are components that are universally distributed throughout cells providing a means to assess independent of culturability.

  14. Fraxinus pollen and allergen concentrations in Ourense (South-western Europe).

    PubMed

    Vara, A; Fernández-González, M; Aira, M J; Rodríguez-Rajo, F J

    2016-05-01

    In temperate zones of North-Central Europe the sensitization to ash pollen is a recognized problem, also extended to the Northern areas of the Mediterranean basin. Some observations in Switzerland suggest that ash pollen season could be as important as birch pollen period. The allergenic significance of this pollen has been poorly studied in Southern Europe as the amounts of ash pollen are low. Due to the high degree of family relationship with the olive pollen major allergen (backed by a sequence identity of 88%), the Fraxinus pollen could be a significant cause of early respiratory allergy in sensitized people to olive pollen as consequence of cross-reactivity processes. Ash tree flowers in the Northwestern Spain during the winter months. The atmospheric presence of Ole e 1-like proteins (which could be related with the Fra a 1 presence) can be accurately detected using Ole e 1 antibodies. The correlation analysis showed high Spearman correlation coefficients between pollen content and rainfall (R(2)=-0.333, p<0.01) or allergen concentration and maximum temperature (R(2)=-0.271, p<0.01). In addiction CCA analysis showed not significant differences (p<0.05) between the component 1 and 2 variables. PCFA analysis plots showed that the allergen concentrations are related to the presence of the Fraxinus pollen in the air, facilitating the wind speed its submicronic allergen proteins dispersion. In order to forecast the Fraxinus allergy risk periods, two regression equations were developed with Adjusted R(2) values around 0.48-0.49. The t-test for dependent samples shows no significant differences between the observed data and the estimated by the equations. The combination of the airborne pollen content and the allergen quantification must be assessed in the epidemiologic study of allergic respiratory diseases. PMID:26901381

  15. Pollen Dispersion, Pollen Viability and Pistil Receptivity in Leymus chinensis

    PubMed Central

    HUANG, ZEHAO; ZHU, JINMAO; MU, XIJIN; LIN, JINXING

    2004-01-01

    • Background and Aims Leymus chinensis is an economically and ecologically important grass that is widely distributed across eastern areas of the Eurasian steppe. A major problem facing its propagation by man is its low sexual reproductivity. The causes of low fecundity are uncertain, largely because many aspects of the reproductive biology of this species remained unknown or incomplete. This study aims to address some of these issues. • Methods Pollen dispersion, pollen viability, pollen longevity and pistil receptivity were studied in a representative, natural population of L. chinensis growing in Inner Mongolia. • Key Results Flowering of L. chinensis occurred at the end of June and lasted for 5 d. Pollination peaked between 1600 h and 1700 h, and about 56·1 % of the total pollen grains were released at this time. Pollen density was highest towards the middle of flowering spikes and lowest at the bottom over the 5 d measurement period. Pollen viability (62·4 %) assessed using TTC was more accurate than using IKI (85·6 %); 50 % of pollen arriving on stigmas germinated. Pollen remained viable for only 3 h and the pollen : ovule ratio was 79 333 : 1. Pistil receptivity lasted for only 3 h and, overall, 86·7 % of pistils were pollinated. Within the spike, the relative fecundity of different positions was middle > lower > upper throughout the period of pollination; daily variation of fecundity was similar to that of the pollen flow. The spikes that opened on the day of highest pollen density exhibited the highest fecundity (36·0 %). No seeds were produced by self‐pollination. • Conclusions The data suggest that low pollen viability, short pollen longevity and short pistil receptivity all appear to contribute to the low seed production typical of this important forage crop. PMID:14744707

  16. Contribution of pollen to atmospheric ice nuclei concentrations

    NASA Astrophysics Data System (ADS)

    Petters, M. D.; Hader, J.; Wright, T.; McMeeking, G. R.

    2013-12-01

    measured by the use of a UV fluorescence Wideband Integrated Bioaerosol Sensor (WIBS-4A) and used to infer the fraction of fluorescent particles that are able to serve as IN. Results show that IN concentrations for nuclei that require minimal supercooling were enhanced for one sample during the pollen season. However, no general trend was observed between ambient pollen counts and observed IN concentrations, suggesting that ice nuclei multiplication via pollen sac rupturing and subsequent release of macromolecules was not prevalent for the pollen types and meteorological conditions typically encountered in the Southeastern U.S.

  17. Associations between weather conditions and ragweed pollen variations in Szeged, Hungary.

    PubMed

    Matyasovszky, István; Makra, László; Csépe, Zoltán

    2012-09-01

    This study analyses potential associations between day-to-day variations in common ragweed pollen counts in the southern Hungarian district of Szeged and meteorological variables using adapted factor analysis. The database includes ten years (1997-2006) worth of data on daily common ragweed pollen ratios (value on the given day per value on the day before) and daily differences (value on the given day minus value on the day before) in eight meteorological variables (mean temperature, minimum temperature, maximum temperature, temperature range, irradiance, relative humidity, wind speed, and rainfall) over the ragweed pollen season. This method is new, as it has only been applied in the economics. In factor analysis it is advisable to combine all the weights of the factors and the resultant variable into one factor indicating the rank of importance of the given explanatory variables in influencing the resultant variable, while the remaining factors are uncorrelated with the resultant variable. The procedure shows that wind speed, rainfall, and temperature range are the most important, while minimum temperature and irradiance are the least important meteorological variables influencing daily pollen ratios. We found a tendency to stronger associations between the meteorological variables and the pollen variable when the pollen ratio was 1 or below. This is due to the fact that data corresponding to the pollen ratio over 1 come mainly from the prepeak pollen season, while data corresponding to less than 1 are characteristic of the post-peak pollen season (late summer to early autumn). PMID:23152381

  18. Asthma admissions and thunderstorms: a study of pollen, fungal spores, rainfall, and ozone.

    PubMed

    Anderson, W; Prescott, G J; Packham, S; Mullins, J; Brookes, M; Seaton, A

    2001-08-01

    Asthma admissions have been reported to increase during thunderstorms. In some cases, this has been attributed to rises in pollen or fungal spore counts occurring alone or in combination with rainfall. We tested the hypothesis that thunderstorms in general are associated with asthma admissions, and investigated the possible roles of pollen, fungal spores, ozone, and other meteorological factors. We obtained data on multiple pollen and fungal spore counts, rainfall, temperature, ambient ozone concentrations, and asthma admissions for 32 dates when lightning strikes were recorded in the Cardiff/Newport area, and 64 matched dates in previous and subsequent years. Poisson regression models were used to investigate associations between admissions and proposed causative environmental factors. The number of asthma admissions was greater on days with thunderstorms than on control days (p<0.001). There were no associations or interactions between admissions and any pollen or fungal spore counts or rainfall. After adjusting for thunderstorms, there was an independent association between increasing ozone concentration, when temperature was included in the model, and increasing admissions (p=0.02). Asthma admissions are increased during thunderstorms. The effect is more marked in warmer weather, and is not explained by increases in grass pollen, total pollen or fungal spore counts, nor by an interaction between these and rainfall. There was an independent, positive association between ozone concentrations and asthma admissions. PMID:11493720

  19. Fourth Airborne Geoscience Workshop

    NASA Technical Reports Server (NTRS)

    1991-01-01

    The focus of the workshop was on how the airborne community can assist in achieving the goals of the Global Change Research Program. The many activities that employ airborne platforms and sensors were discussed: platforms and instrument development; airborne oceanography; lidar research; SAR measurements; Doppler radar; laser measurements; cloud physics; airborne experiments; airborne microwave measurements; and airborne data collection.

  20. Trans-disciplinary research in synthesis of grass pollen aerobiology and its importance for respiratory health in Australasia.

    PubMed

    Davies, Janet M; Beggs, Paul J; Medek, Danielle E; Newnham, Rewi M; Erbas, Bircan; Thibaudon, Michel; Katelaris, Connstance H; Haberle, Simon G; Newbigin, Edward J; Huete, Alfredo R

    2015-11-15

    Grass pollen is a major trigger for allergic rhinitis and asthma, yet little is known about the timing and levels of human exposure to airborne grass pollen across Australasian urban environments. The relationships between environmental aeroallergen exposure and allergic respiratory disease bridge the fields of ecology, aerobiology, geospatial science and public health. The Australian Aerobiology Working Group comprised of experts in botany, palynology, biogeography, climate change science, plant genetics, biostatistics, ecology, pollen allergy, public and environmental health, and medicine, was established to systematically source, collate and analyse atmospheric pollen concentration data from 11 Australian and six New Zealand sites. Following two week-long workshops, post-workshop evaluations were conducted to reflect upon the utility of this analysis and synthesis approach to address complex multidisciplinary questions. This Working Group described i) a biogeographically dependent variation in airborne pollen diversity, ii) a latitudinal gradient in the timing, duration and number of peaks of the grass pollen season, and iii) the emergence of new methodologies based on trans-disciplinary synthesis of aerobiology and remote sensing data. Challenges included resolving methodological variations between pollen monitoring sites and temporal variations in pollen datasets. Other challenges included "marrying" ecosystem and health sciences and reconciling divergent expert opinion. The Australian Aerobiology Working Group facilitated knowledge transfer between diverse scientific disciplines, mentored students and early career scientists, and provided an uninterrupted collaborative opportunity to focus on a unifying problem globally. The Working Group provided a platform to optimise the value of large existing ecological datasets that have importance for human respiratory health and ecosystems research. Compilation of current knowledge of Australasian pollen aerobiology

  1. Airborne laser

    NASA Astrophysics Data System (ADS)

    Lamberson, Steven E.

    2002-06-01

    The US Air Force Airborne Laser (ABL) is an airborne, megawatt-class laser system with a state-of-the-art atmospheric compensation system to destroy enemy ballistic missiles at long ranges. This system will provide both deterrence and defense against the use of such weapons during conflicts. This paper provides an overview of the ABL weapon system including: the notional operational concept, the development approach and schedule, the overall aircraft configuration, the technologies being incorporated in the ABL, and the risk reduction approach being utilized to ensure program success.

  2. Effect of Palm Pollen on Sperm Parameters of Infertile Man.

    PubMed

    Rasekh, Athar; Jashni, Hojjatollah Karimi; Rahmanian, Karamatollah; Jahromi, Abdolreza Sotoodeh

    2015-04-01

    There is a rapidly growing trend in the consumption of herbal remedies in the developing countries. The aim of this study was to determine the effects of orally administered Date Palm Pollen (DPP) on the results of semen analysis in adult infertile men. Forty infertile men participated in our study. They were treated by Pollen powder 120 mg kg(-1) in gelatinous capsules every other day, for two months. Before and at the end of therapy, the semen was collected after masturbation and sperm numbers, motility and morphology were determined. Our findings revealed that consumption of DPP improved the sperm count. The treatment was significantly increased sperm motility, morphology and forward progressive motility. Date palm pollen seems to cure male infertility by improving the quality of sperm parameters. PMID:26506651

  3. Fungal spores and pollen in particulate matter collected during agricultural activities in the Po Valley (Italy).

    PubMed

    Telloli, Chiara; Chicca, Milvia; Leis, Marilena; Vaccaro, Carmela

    2016-08-01

    Airborne particulate matter (PM) containing fungal spores and pollen grains was sampled within a monitoring campaign of wheat threshing, plowing and sowing agricultural operations. Fungal spores and pollen grains were detected and identified on morphological basis. No studies were previously available about fungal spore and pollen content in agricultural PM in the Po Valley. Sampling was conducted in a Po Valley farmland in Mezzano (Ferrara, Italy). The organic particles collected were examined by scanning electron microscopy with energy dispersive X-ray spectrometer. Fungal spores and pollen grains were identified when possible at the level of species. The most frequent components of the organic particles sampled were spores of Aspergillus sp., which could represent a risk of developing allergies and aspergillosis for crop farmers. PMID:27521955

  4. Comparative analysis of two sampling techniques for pollen gathered by Nannotrigona testaceicornis Lepeletier (Apidae, Meliponini).

    PubMed

    Malagodi-Braga, K S; Kleinert, A M P

    2009-01-01

    Pollen counts from samples taken from storage pots throughout one year (from October to September) were adjusted by Tasei's volumetric correction coefficient for the determination of pollen sources exploited by two colonies of Nannotrigona testaceicornis in São Paulo, Brazil. The results obtained by this sampling technique for seven months (December to June) were compared with those from corbicula load samples taken within the same period. This species visited a large variety of plant species, but few of them were frequently used. As a rule, pollen sources that appeared at frequencies greater than 1% were found with both sampling methods and significant positive correlations (Spearman correlation coefficient) were found between their values. The pollen load sample data showed that N. testaceicornis gathered pollen throughout the external activity period. PMID:19551648

  5. Influence of wind velocity on pollen concentration in urban canopy layer

    NASA Astrophysics Data System (ADS)

    Pospisil, J.; Jícha, M.

    2009-09-01

    POLLEN RELEASE Temperature is the basic parameter for prediction of the beginning of the pollen season and identification days with good potential for pollen release. Different approaches are used for determination of the start of the pollen season: i) the sum of daily pollen counts = x criterion (Arnold 2002), ii) the mean temperature method during pre-defined period (Sparks, 2000), iii) the temperature sum method (Jones 1992). Another parameters influencing pollen release are: day light length, morning temperature gradient, relative humidity. The mentioned parameters enable to create the "statistical” model for determination of timing of pollen potential release. But, the correct determination of pollen release timing is only the first step to correct prediction of pollen concentration in air. The above mentioned collection of parameters isn't complete for correct pollen production prediction without inclusion of the actual wind velocity. The wind velocity directly influences the pollen release rate from mother plant and subsequently transport of pollen grains. From this reason, influence of wind conditions has to be considered as exactly as possible in complex prediction models. WIND VELOCITY AND POLLEN CONCENTRATION Results of in-situ measurements were used for carried out analysis of the relation between wind velocity and pollen concentration in an urban canopy layer. The mean daily wind velocities and the mean daily pollen concentrations were used as the input data describing the pollen season 2005 in an inner part of the city of Brno (pop. 400 000). The mean daily pollen concentrations were matched to corresponding mean daily wind velocity and depicted in graphs. This procedure was done for all locally monitored aeroallergens, namely Alnus, Ambrosia, Betula, Artemis, Corylus, Fraxinus, Poaceae and Quercus. Only days with significant pollen concentration (above 10% of maximal pollen season concentration) were considered for detail analysis. Clear

  6. A PLAUSIBLE ROLE FOR POLLEN-RESIDING MOLDS IN AGRICULTURAL PURPOSES.

    PubMed

    Belhadj, H; Harzallah, D; Dahamna, S; Ghadbane, M

    2015-01-01

    Pollen microbial content of 15 samples was investigated. Pollen was collected by honeybees. Total aerobic mesophilic count ranged from 3.00 to 5.48 Log CFU/g. Total mold and yeast count ranged from 2.3 to 6.99 Log CFU/g. Selected strains of isolated molds from pollen samples were characterized by conventional methods. Potent phytopathogenic and food spoilage species such as Penicillium sp., Alternaria alternata, Alternaria sp., Cladosporium werneckii, Mucor hiemalis, Rhizomucor pusillus, Aspergillus flavus, Aspergillus niger, Drechslera tritici-repentis, Verticillium albo-atrum, and Aspergillus alliaceus were recovered. Other fungal species with valuable biotechnological and plant diseases control purposes were isolated. They were characterized as Geotrichum candidum, Monilia sitophilia, and Sepedonium chrysospermum. Animal pathogenic molds were also isolated. Bee pollen may be considered as a source for a highly diverse fungal flora with different applications. PMID:27141752

  7. Multiplicity Counting

    SciTech Connect

    Geist, William H.

    2015-12-01

    This set of slides begins by giving background and a review of neutron counting; three attributes of a verification item are discussed: 240Pueff mass; α, the ratio of (α,n) neutrons to spontaneous fission neutrons; and leakage multiplication. It then takes up neutron detector systems – theory & concepts (coincidence counting, moderation, die-away time); detector systems – some important details (deadtime, corrections); introduction to multiplicity counting; multiplicity electronics and example distributions; singles, doubles, and triples from measured multiplicity distributions; and the point model: multiplicity mathematics.

  8. Climate change effect on Betula (birch) and Quercus (oak) pollen seasons in the United States

    NASA Astrophysics Data System (ADS)

    Zhang, Yong; Bielory, Leonard; Georgopoulos, Panos G.

    2014-07-01

    Climatic change is expected to affect the spatiotemporal patterns of airborne allergenic pollen, which has been found to act synergistically with common air pollutants, such as ozone, to cause allergic airway disease (AAD). Observed airborne pollen data from six stations from 1994 to 2011 at Fargo (North Dakota), College Station (Texas), Omaha (Nebraska), Pleasanton (California), Cherry Hill and Newark (New Jersey) in the US were studied to examine climate change effects on trends of annual mean and peak value of daily concentrations, annual production, season start, and season length of Betula (birch) and Quercus (oak) pollen. The growing degree hour (GDH) model was used to establish a relationship between start/end dates and differential temperature sums using observed hourly temperatures from surrounding meteorology stations. Optimum GDH models were then combined with meteorological information from the Weather Research and Forecasting (WRF) model, and land use land coverage data from the Biogenic Emissions Land use Database, version 3.1 (BELD3.1), to simulate start dates and season lengths of birch and oak pollen for both past and future years across the contiguous US (CONUS). For most of the studied stations, comparison of mean pollen indices between the periods of 1994-2000 and 2001-2011 showed that birch and oak trees were observed to flower 1-2 weeks earlier; annual mean and peak value of daily pollen concentrations tended to increase by 13.6 %-248 %. The observed pollen season lengths varied for birch and for oak across the different monitoring stations. Optimum initial date, base temperature, and threshold GDH for start date was found to be 1 March, 8 °C, and 1,879 h, respectively, for birch; 1 March, 5 °C, and 4,760 h, respectively, for oak. Simulation results indicated that responses of birch and oak pollen seasons to climate change are expected to vary for different regions.

  9. Climate change effect on Betula (birch) and Quercus (oak) pollen seasons in the United States.

    PubMed

    Zhang, Yong; Bielory, Leonard; Georgopoulos, Panos G

    2014-07-01

    Climatic change is expected to affect the spatiotemporal patterns of airborne allergenic pollen, which has been found to act synergistically with common air pollutants, such as ozone, to cause allergic airway disease (AAD). Observed airborne pollen data from six stations from 1994 to 2011 at Fargo (North Dakota), College Station (Texas), Omaha (Nebraska), Pleasanton (California), Cherry Hill and Newark (New Jersey) in the US were studied to examine climate change effects on trends of annual mean and peak value of daily concentrations, annual production, season start, and season length of Betula (birch) and Quercus (oak) pollen. The growing degree hour (GDH) model was used to establish a relationship between start/end dates and differential temperature sums using observed hourly temperatures from surrounding meteorology stations. Optimum GDH models were then combined with meteorological information from the Weather Research and Forecasting (WRF) model, and land use land coverage data from the Biogenic Emissions Land use Database, version 3.1 (BELD3.1), to simulate start dates and season lengths of birch and oak pollen for both past and future years across the contiguous US (CONUS). For most of the studied stations, comparison of mean pollen indices between the periods of 1994-2000 and 2001-2011 showed that birch and oak trees were observed to flower 1-2 weeks earlier; annual mean and peak value of daily pollen concentrations tended to increase by 13.6%-248%. The observed pollen season lengths varied for birch and for oak across the different monitoring stations. Optimum initial date, base temperature, and threshold GDH for start date was found to be 1 March, 8 °C, and 1,879 h, respectively, for birch; 1 March, 5 °C, and 4,760 h, respectively, for oak. Simulation results indicated that responses of birch and oak pollen seasons to climate change are expected to vary for different regions. PMID:23793955

  10. High sensitive airborne radioiodine monitor.

    PubMed

    Ogata, Yoshimune; Yamasaki, Tadashi; Hanafusa, Ryuji

    2013-11-01

    Airborne radioiodine monitoring includes a problem in that commercial radioactive gas monitors have inadequate sensitivity. To solve this problem, we designed a highly sensitive monitoring system. The higher counting efficiency and lower background made it possible to perform the low-level monitoring. The characteristics of the system were investigated using gaseous (125)I. The minimum detectable activity concentration was 1 × 10(-4)Bq cm(-3) for 1 min counting, which is one tenth of the legal limit for the radiation controlled areas in Japan. PMID:23602709

  11. Grass pollen hypersensitivity in mice

    PubMed Central

    McCaskill, A. C.; Hosking, C. S.; Hill, D. J.

    1982-01-01

    Mice were sensitized by intranasal administration of ryegrass pollen. Subsequent nasal challenge with pollen extract led to a `shock' response peaking in severity 4 hr after challenge. Histological examination of lungs revealed the development of a pneumonitis which was most severe 3 days after challenge. ImagesFigure 2 PMID:7106842

  12. The effects of bee pollen extracts on the broiler chicken's gastrointestinal microflora.

    PubMed

    Kačániová, Miroslava; Rovná, Katarína; Arpášová, Henrieta; Hleba, Lukáš; Petrová, Jana; Haščík, Peter; Cuboň, Juraj; Pavelková, Adriana; Chlebo, Róbert; Bobková, Alica; Stričík, Michal

    2013-08-01

    The aim of this study was to investigate the effects of bee pollen ethanolic extracts on the in vivo gastrointestinal tract microflora colonization of broiler chickens. A completely randomized experiment based on six treatments (different concentrations of bee pollen - 0, 5, 15, 25, 35 and 45 g kg(-1) diet) was used during 7 weeks. The highest count of faecal Enterococci was found in the experimental group with the addition of 15 g of pollen (8.85 ± 0.87 log CFU g(-1)) per 1 kg of feed mixture. The highest count of Lactobacilli was detected in the experimental group with 35 g of pollen per 1 kg of feed mixture and the highest number of the Enterobacteriaceae genera count was found in the control group (8.43 ± 0.15 log CFU g(-1)). Moreover, the MALDI TOF MS Biotyper identified the following genera: Escherichia coli, Proteus mirabilis, Klebsiella oxytoca, as well as Lactobacillus acidophilus, L. crispatus, L. fermentum and L. salivarius from the Lactobacilli group and Enterococcus avium, E. casseliflavus, E. cecorum, E. faecalis, E. faecium, E. gallinarum, E. hirae and E. malodoratus from the Enterococci group. Additionally, the in vitro antimicrobial activities of pollen against five bacteria species isolated from gastrointestinal tracts of chickens were tested. The best antimicrobial effect of the pollen extract was detected against K. oxytoca. PMID:23548478

  13. Trends in the characteristics of allergenic pollen circulation in central Europe based on the example of Szeged, Hungary

    NASA Astrophysics Data System (ADS)

    Makra, László; Matyasovszky, István; Deák, Áron József

    2011-10-01

    The aim of the study is to analyse trends of the pollination season with its start and end dates, as well as trends of the annual total pollen count and annual peak pollen concentration for the Szeged agglomeration in Southern Hungary. The data set covers an 11-year period (1999-2009) and includes one of the largest spectra, with 19 taxa, as well as seven meteorological variables (minimum-, maximum- and mean temperature, total radiation, relative humidity, rainfall and wind speed). For highly skewed data, such as the annual total number of pollen counts or annual peak pollen concentrations, the Mann-Kendall test has a substantially greater predictive power than the t-test. After performing Mann-Kendall tests, the annual cycles of daily slopes of pollen concentration trends and annual cycles of daily slopes of climate variable trends are calculated. This kind of trend analysis is a novel approach as it provides information on annual cycles of trends. In order to represent the strength of their relationships an association measure (AM) and a multiple association measure (MAM) are introduced. Based on climate sensitivity, the individual taxa are sorted into three categories. The results obtained for the pollen quantity and phenological characteristics are compared with two novel climate change related categories, namely risk and expansion potential due to the climate change for each taxon. The total annual pollen count and annual peak pollen concentrations indicate a small number of changes when using ordinary linear trends, while the total annual pollen count calculated via daily linear trends show significant trends (70% of them positive) for almost all taxa. However, except for Poaceae and Urtica, there is no significant change in the duration of the pollination season. The association measure performs well compared to the climate change related forces. Furthermore, remarkable changes in pollen season characteristics are also in accordance with the risk and

  14. Glutathione-S-Transferase: A Minor Allergen in Birch Pollen due to Limited Release from Hydrated Pollen

    PubMed Central

    Vejvar, Eva; Kitzmüller, Claudia; Gadermaier, Gabriele; Nagl, Birgit; Vrtala, Susanne; Briza, Peter; Zlabinger, Gerhard J.; Jahn-Schmid, Beatrice; Ferreira, Fatima; Bohle, Barbara

    2014-01-01

    Background Recently, a protein homologous to glutathione-S-transferases (GST) was detected in prominent amounts in birch pollen by proteomic profiling. As members of the GST family are relevant allergens in mites, cockroach and fungi we investigated the allergenic relevance of GST from birch (bGST). Methodology bGST was expressed in Escherichia coli, purified and characterized by mass spectrometry. Sera from 217 birch pollen-allergic patients were tested for IgE-reactivity to bGST by ELISA. The mediator-releasing activity of bGST was analysed with IgE-loaded rat basophil leukaemia cells (RBL) expressing human FcεRI. BALB/c mice were immunized with bGST or Bet v 1. Antibody and T cell responses to either protein were assessed. IgE-cross-reactivity between bGST with GST from house dust mite, Der p 8, was studied with murine and human sera in ELISA. The release kinetics of bGST and Bet v 1 from birch pollen were assessed in water, simulated lung fluid, 0.9% NaCl and PBS. Eluted proteins were quantified by ELISA and analysed by immunoblotting. Principle findings Only 13% of 217 birch pollen-allergic patients showed IgE-reactivity to bGST. In RBL assays bGST induced mediator release. Immunization of mice with bGST induced specific IgE and a Th2-dominated cellular immune response comparably to immunization with Bet v 1. bGST did not cross-react with Der p 8. In contrast to Bet v 1, only low amounts of bGST were released from pollen grains upon incubation in water and the different physiological solutions. Conclusion/Significance Although bGST is abundant in birch pollen, immunogenic in mice and able to induce mediator release from effector cells passively loaded with specific IgE, it is a minor allergen for birch pollen-allergic patients. We refer this discrepancy to its limited release from hydrated pollen. Hence, bGST is an example demonstrating that allergenicity depends mainly on rapid elution from airborne particles. PMID:25275548

  15. CHARACTERIZATION OF THE MAIZE POLLEN TRANSCRIPTOME

    EPA Science Inventory

    Pollen is a primary vehicle for transgene flow from engineered plants to their non-transgenic, native or weedy relatives. Hence, gene flow will be affected by pollen fitness (e.g., how well a particular pollen grain can outcompete other pollen present on the stigma and complete ...

  16. City scale pollen concentration variability

    NASA Astrophysics Data System (ADS)

    van der Molen, Michiel; van Vliet, Arnold; Krol, Maarten

    2016-04-01

    Pollen are emitted in the atmosphere both in the country-side and in cities. Yet the majority of the population is exposed to pollen in cities. Allergic reactions may be induced by short-term exposure to pollen. This raises the question how variable pollen concentration in cities are in temporally and spatially, and how much of the pollen in cities are actually produced in the urban region itself. We built a high resolution (1 × 1 km) pollen dispersion model based on WRF-Chem to study a city's pollen budget and the spatial and temporal variability in concentration. It shows that the concentrations are highly variable, as a result of source distribution, wind direction and boundary layer mixing, as well as the release rate as a function of temperature, turbulence intensity and humidity. Hay Fever Forecasts based on such high resolution emission and physical dispersion modelling surpass traditional hay fever warning methods based on temperature sum methods. The model gives new insights in concentration variability, personal and community level exposure and prevention. The model will be developped into a new forecast tool to serve allergic people to minimize their exposure and reduce nuisance, coast of medication and sick leave. This is an innovative approach in hay fever warning systems.

  17. Development and validation of a 5-day-ahead hay fever forecast for patients with grass-pollen-induced allergic rhinitis

    NASA Astrophysics Data System (ADS)

    de Weger, Letty A.; Beerthuizen, Thijs; Hiemstra, Pieter S.; Sont, Jacob K.

    2014-08-01

    One-third of the Dutch population suffers from allergic rhinitis, including hay fever. In this study, a 5-day-ahead hay fever forecast was developed and validated for grass pollen allergic patients in the Netherlands. Using multiple regression analysis, a two-step pollen and hay fever symptom prediction model was developed using actual and forecasted weather parameters, grass pollen data and patient symptom diaries. Therefore, 80 patients with a grass pollen allergy rated the severity of their hay fever symptoms during the grass pollen season in 2007 and 2008. First, a grass pollen forecast model was developed using the following predictors: (1) daily means of grass pollen counts of the previous 10 years; (2) grass pollen counts of the previous 2-week period of the current year; and (3) maximum, minimum and mean temperature ( R 2 = 0.76). The second modeling step concerned the forecasting of hay fever symptom severity and included the following predictors: (1) forecasted grass pollen counts; (2) day number of the year; (3) moving average of the grass pollen counts of the previous 2 week-periods; and (4) maximum and mean temperatures ( R 2 = 0.81). Since the daily hay fever forecast is reported in three categories (low-, medium- and high symptom risk), we assessed the agreement between the observed and the 1- to 5-day-ahead predicted risk categories by kappa, which ranged from 65 % to 77 %. These results indicate that a model based on forecasted temperature and grass pollen counts performs well in predicting symptoms of hay fever up to 5 days ahead.

  18. Development and validation of a 5-day-ahead hay fever forecast for patients with grass-pollen-induced allergic rhinitis.

    PubMed

    de Weger, Letty A; Beerthuizen, Thijs; Hiemstra, Pieter S; Sont, Jacob K

    2014-08-01

    One-third of the Dutch population suffers from allergic rhinitis, including hay fever. In this study, a 5-day-ahead hay fever forecast was developed and validated for grass pollen allergic patients in the Netherlands. Using multiple regression analysis, a two-step pollen and hay fever symptom prediction model was developed using actual and forecasted weather parameters, grass pollen data and patient symptom diaries. Therefore, 80 patients with a grass pollen allergy rated the severity of their hay fever symptoms during the grass pollen season in 2007 and 2008. First, a grass pollen forecast model was developed using the following predictors: (1) daily means of grass pollen counts of the previous 10 years; (2) grass pollen counts of the previous 2-week period of the current year; and (3) maximum, minimum and mean temperature (R (2)=0.76). The second modeling step concerned the forecasting of hay fever symptom severity and included the following predictors: (1) forecasted grass pollen counts; (2) day number of the year; (3) moving average of the grass pollen counts of the previous 2 week-periods; and (4) maximum and mean temperatures (R (2)=0.81). Since the daily hay fever forecast is reported in three categories (low-, medium- and high symptom risk), we assessed the agreement between the observed and the 1- to 5-day-ahead predicted risk categories by kappa, which ranged from 65 % to 77 %. These results indicate that a model based on forecasted temperature and grass pollen counts performs well in predicting symptoms of hay fever up to 5 days ahead. PMID:23780494

  19. Counting Penguins.

    ERIC Educational Resources Information Center

    Perry, Mike; Kader, Gary

    1998-01-01

    Presents an activity on the simplification of penguin counting by employing the basic ideas and principles of sampling to teach students to understand and recognize its role in statistical claims. Emphasizes estimation, data analysis and interpretation, and central limit theorem. Includes a list of items for classroom discussion. (ASK)

  20. RBC count

    MedlinePlus

    ... Drugs that can increase the RBC count include: Gentamicin Methyldopa Lower-than-normal numbers of RBCs may be due to: Anemia Bleeding Bone marrow failure (for example, from radiation, toxins, or tumor) Deficiency of a hormone called erythropoietin (caused by ...

  1. Counting Populations

    ERIC Educational Resources Information Center

    Damonte, Kathleen

    2004-01-01

    Scientists use sampling to get an estimate of things they cannot easily count. A population is made up of all the organisms of one species living together in one place at the same time. All of the people living together in one town are considered a population. All of the grasshoppers living in a field are a population. Scientists keep track of the…

  2. Grass Pollen Allergens

    PubMed Central

    Augustin, Rosa; Hayward, Barbara J.

    1962-01-01

    Cocksfoot and Timothy pollen extracts are each found to contain at least fifteen components antigenic in rabbits. Most of these can also be allergens for man, but only a few are regularly so. These `principal' allergens have now been isolated in highly purified form. Procedures are given for a simple method of preparing extracts for clinical purposes and for the partial separation, concentration and purification of the allergens by means of differential extractions of the pollens and by means of ultrafiltration, isoelectric precipitation and salt fractionations (at acid and neutral pH) of the extracts. Isoelectric precipitations gave highly pigmented acid complexes, two of which moved as single sharp peaks at pH 7.4 in free electrophoresis, but proved to be hardly active by skin tests. Acid NaCl fractionation of the remainder resulted for Cocksfoot and Timothy in the isolation of a nearly white powder (T21.111121112 = T21B) which was weight for weight 1000–10,000 times as active as the pollen from which it had been derived. The powders have retained their activity for 7 years. By gel diffusion tests, they were found to contain two antigens (one in each preparation) which were immunologically partially related, but the Timothy preparation contained in addition the `innermost' `twin' antigens specific for Timothy that we had discovered previously in the crude extracts by gel diffusion methods. Skin reactions could be elicited in hay-fever subjects by prick tests with concentrations of 10-9–10-8 g./ml., which is equivalent to intradermal injections of 10-11–10-10 mg. and represents a 300-fold purification with respect to the concentrates of crude pollen extracts prepared by ultrafiltration and dialysis. Fractionation on DEAE-cellulose of one of the highly purified Timothy preparations (T21.11112112 = T21A) and other, crude Timothy and Cocksfoot extracts resulted in considerable and reproducible separation of the various antigens, with no indication of the

  3. Does bee pollen cause to eosinophilic gastroenteropathy?

    PubMed Central

    Güç, Belgin Usta; Asilsoy, Suna; Canan, Oğuz; Kayaselçuk, Fazilet

    2015-01-01

    Bee pollen is given to children by mothers in order to strengthen their immune systems. There are no studies related with the side effects of bee polen in the literature. In this article, the literature was reviewed by presenting a case of allergic eosinophilic gastropathy related with bee polen. A 5-year old child was admitted due to abdominal pain. Edema was detected on the eyelids and pretibial region. In laboratory investigations, pathology was not detected in terms of hepatic and renal causes that would explain the protein loss of the patient diagnosed with hypoproteinemia and hypoalbuminemia. Urticaria was detected during the follow-up visit. When the history of the patient was deepened, it was learned that bee pollen was given to the patient every day. The total eosinophil count was found to be 1 800/mm3. Allergic gastroenteropathy was considered because of hypereosinophilia and severe abdominal pain and endoscopy was performed. Biopsy revealed abundant eosinophils in the whole gastric mucosa. A diagnosis of allergic eosinophilic gastropathy was made. Bee polen was discontinued. Abdominal pain and edema disappeared in five days. Four weeks later, the levels of serum albumin and total eosinophil returned to normal. PMID:26568697

  4. Does bee pollen cause to eosinophilic gastroenteropathy?

    PubMed

    Güç, Belgin Usta; Asilsoy, Suna; Canan, Oğuz; Kayaselçuk, Fazilet

    2015-09-01

    Bee pollen is given to children by mothers in order to strengthen their immune systems. There are no studies related with the side effects of bee polen in the literature. In this article, the literature was reviewed by presenting a case of allergic eosinophilic gastropathy related with bee polen. A 5-year old child was admitted due to abdominal pain. Edema was detected on the eyelids and pretibial region. In laboratory investigations, pathology was not detected in terms of hepatic and renal causes that would explain the protein loss of the patient diagnosed with hypoproteinemia and hypoalbuminemia. Urticaria was detected during the follow-up visit. When the history of the patient was deepened, it was learned that bee pollen was given to the patient every day. The total eosinophil count was found to be 1 800/mm(3). Allergic gastroenteropathy was considered because of hypereosinophilia and severe abdominal pain and endoscopy was performed. Biopsy revealed abundant eosinophils in the whole gastric mucosa. A diagnosis of allergic eosinophilic gastropathy was made. Bee polen was discontinued. Abdominal pain and edema disappeared in five days. Four weeks later, the levels of serum albumin and total eosinophil returned to normal. PMID:26568697

  5. Ragweed pollen production and dispersion modelling within a regional climate system, calibration and application over Europe

    NASA Astrophysics Data System (ADS)

    Liu, Li; Solmon, Fabien; Vautard, Robert; Hamaoui-Laguel, Lynda; Zsolt Torma, Csaba; Giorgi, Filippo

    2016-05-01

    Common ragweed (Ambrosia artemisiifolia L.) is a highly allergenic and invasive plant in Europe. Its pollen can be transported over large distances and has been recognized as a significant cause of hay fever and asthma (D'Amato et al., 2007; Burbach et al., 2009). To simulate production and dispersion of common ragweed pollen, we implement a pollen emission and transport module in the Regional Climate Model (RegCM) version 4 using the framework of the Community Land Model (CLM) version 4.5. In this online approach pollen emissions are calculated based on the modelling of plant distribution, pollen production, species-specific phenology, flowering probability, and flux response to meteorological conditions. A pollen tracer model is used to describe pollen advective transport, turbulent mixing, dry and wet deposition. The model is then applied and evaluated on a European domain for the period 2000-2010. To reduce the large uncertainties notably due to the lack of information on ragweed density distribution, a calibration based on airborne pollen observations is used. Accordingly a cross validation is conducted and shows reasonable error and sensitivity of the calibration. Resulting simulations show that the model captures the gross features of the pollen concentrations found in Europe, and reproduce reasonably both the spatial and temporal patterns of flowering season and associated pollen concentrations measured over Europe. The model can explain 68.6, 39.2, and 34.3 % of the observed variance in starting, central, and ending dates of the pollen season with associated root mean square error (RMSE) equal to 4.7, 3.9, and 7.0 days, respectively. The correlation between simulated and observed daily concentrations time series reaches 0.69. Statistical scores show that the model performs better over the central Europe source region where pollen loads are larger and the model is better constrained. From these simulations health risks associated to common ragweed pollen

  6. AIRBORNE-CONTACT DERMATITIS OF NON-PLANT ORIGIN: AN OVERVIEW

    PubMed Central

    Ghosh, Sanjay

    2011-01-01

    Airborne-contact dermatitis (ABCD) represents a unique type of contact dermatitis originating from dust, sprays, pollens or volatile chemicals by airborne fumes or particles without directly touching the allergen. ABCD in Indian patients has been attributed exclusively by pollens of the plants like Parthenium hysterophorus, etc., but in recent years the above scenario has been changing rapidly in urban and semiurban perspective especially in developing countries. ABCD has been reported worldwide due to various type of nonplant allergens and their clinical feature are sometimes distinctive. Preventive aspect has been attempted by introduction of different chemicals of less allergic potential. PMID:22345776

  7. Trends in atmospheric concentrations of weed pollen in the context of recent climate warming in Poznań (Western Poland)

    NASA Astrophysics Data System (ADS)

    Bogawski, Paweł; Grewling, Łukasz; Nowak, Małgorzata; Smith, Matt; Jackowiak, Bogdan

    2014-10-01

    A significant increase in summer temperatures has been observed for the period 1996-2011 in Poznań, Poland. The phenological response of four weed taxa, widely represented by anemophilous species ( Artemisia spp., Rumex spp. and Poaceae and Urticaceae species) to this recent climate warming has been analysed in Poznań by examining the variations in the course of airborne pollen seasons. Pollen data were collected by 7-day Hirst-type volumetric trap. Trends in pollen seasons were determined using Mann-Kendall test and Sen's slope estimator, whereas the relationships between meteorological and aerobiological data were established by Spearman's rank correlation coefficient. Significant trends in pollen data were detected. The duration of pollen seasons of all analysed taxa increased (from +2.0 days/year for Urticaceae to +3.8 days/year for Rumex), which can be attributed to a delay in pollen season end dates rather than earlier start dates. In addition, the intensity of Artemisia pollen seasons significantly decreased and correlates with mean July-September daily minimum temperatures ( r = -0.644, p < 0.01). In contrast, no significant correlations were found between temperature and characteristics of Rumex pollen seasons. The results of this study show that observed shifts in weed pollen seasons in Poznań, i.e. longer duration and later end dates, might be caused by the recorded increase in summer temperature. This influence was the strongest in relation to Artemisia, which is the taxon that flowers latest in the year. The general lack of significant correlations between Rumex and Urticaceae pollen seasons and spring and/or summer temperature suggests that other factors, e.g. land use practices, could also be partially responsible for the observed shifts in pollen seasons.

  8. A new method for determining the sources of airborne particles.

    PubMed

    Oteros, J; García-Mozo, H; Alcázar, P; Belmonte, J; Bermejo, D; Boi, M; Cariñanos, P; Díaz de la Guardia, C; Fernández-González, D; González-Minero, F; Gutiérrez-Bustillo, A M; Moreno-Grau, S; Pérez-Badía, R; Rodríguez-Rajo, F J; Ruíz-Valenzuela, L; Suárez-Pérez, J; Trigo, M M; Domínguez-Vilches, E; Galán, C

    2015-05-15

    Air quality is a major issue for humans owing to the fact that the content of particles in the atmosphere has multiple implications for life quality, ecosystem dynamics and environment. Scientists are therefore particularly interested in discovering the origin of airborne particles. A new method has been developed to model the relationship between the emission surface and the total amount of airborne particles at a given distance, employing olive pollen and olive groves as examples. A third-degree polynomial relationship between the air particles at a particular point and the distance from the source was observed, signifying that the nearest area to a point is not that which is most correlated with its air features. This work allows the origin of airborne particles to be discovered and could be implemented in different disciplines related to atmospheric aerosol, thus providing a new approach with which to discover the dynamics of airborne particles. PMID:25837296

  9. Pollen Forecast and Dispersion Modelling

    NASA Astrophysics Data System (ADS)

    Costantini, Monica; Di Giuseppe, Fabio; Medaglia, Carlo Maria; Travaglini, Alessandro; Tocci, Raffaella; Brighetti, M. Antonia; Petitta, Marcello

    2014-05-01

    The aim of this study is monitoring, mapping and forecast of pollen distribution for the city of Rome using in-situ measurements of 10 species of common allergenic pollens and measurements of PM10. The production of daily concentration maps, associated to a mobile phone app, are innovative compared to existing dedicated services to people who suffer from respiratory allergies. The dispersal pollen is one of the most well-known causes of allergic disease that is manifested by disorders of the respiratory functions. Allergies are the third leading cause of chronic disease and it is estimated that tens millions of people in Italy suffer from it. Recent works reveal that during the last few years there was a progressive increase of affected subjects, especially in urban areas. This situation may depend: on the ability to transport of pollutants, on the ability to react between pollutants and pollen and from a combination of other irritants, existing in densely populated and polluted urban areas. The methodology used to produce maps is based on in-situ measurements time series relative to 2012, obtained from networks of air quality and pollen stations in the metropolitan area of Rome. The monitoring station aerobiological of University of Rome "Tor Vergata" is located at the Department of Biology. The instrument used to pollen monitoring is a volumetric sampler type Hirst (Hirst 1952), Model 2000 VPPS Lanzoni; the data acquisition is carried out as reported in Standard UNI 11008:2004 - "Qualità dell'aria - Metodo di campionamento e conteggio dei granuli pollinici e delle spore fungine aerodisperse" - the protocol that describes the procedure for measuring of the concentration of pollen grains and fungal spores dispersed into the atmosphere, and reported in the "Manuale di gestione e qualità della R.I.M.A" (Travaglini et. al. 2009). All 10 allergenic pollen are monitored since 1996. At Tor Vergata university is also operating a meteorological station (SP2000, CAE

  10. Pollen taphonomy in a canyon stream

    NASA Astrophysics Data System (ADS)

    Fall, Patricia L.

    1987-11-01

    Surface soil samples from the forested Chuska Mountains to the arid steppe of the Chinle Valley, Northeastern Arizona, show close correlation between modern pollen rain and vegetation. In contrast, modern alluvium is dominated by Pinus pollen throughout the canyon; it reflects neither the surrounding floodplain nor plateau vegetation. Pollen in surface soils is deposited by wind; pollen grains in alluvium are deposited by a stream as sedimentary particles. Clay-size particles correlate significantly with Pinus, Quercus, and Populus pollen. These pollen types settle, as clay does, in slack water. Chenopodiaceae- Amaranthus, Artemisia, other Tubuliflorae, and indeterminate pollen types correlate with sand-size particles, and are deposited by more turbulent water. Fluctuating pollen frequencies in alluvial deposits are related to sedimentology and do not reflect the local or regional vegetation where the sediments were deposited. Alluvial pollen is unreliable for reconstruction of paleoenvironments.

  11. Reticulocyte Count Test

    MedlinePlus

    ... Reticulocyte Count Related tests: Red Blood Cell Count ; Hemoglobin ; Hematocrit ; Complete Blood Count ; Blood Smear ; Erythropoietin ; Vitamin ... on a complete blood count (CBC) , RBC count , hemoglobin or hematocrit , to help determine the cause To ...

  12. White Blood Cell Count

    MedlinePlus

    ... Home Visit Global Sites Search Help? White Blood Cell Count Share this page: Was this page helpful? Also ... Leukocyte Count; White Count Formal name: White Blood Cell Count Related tests: Complete Blood Count , Blood Smear , White ...

  13. The long range transport of birch (Betula) pollen from Poland and Germany causes significant pre-season concentrations in Denmark

    NASA Astrophysics Data System (ADS)

    Ambelas Skjoth, C.; Sommer, J.; Stach, A.; Smith, M.; Brandt, J.; Christensen, J. H.; Frohn, L. M.; Geels, C.; Hansen, K. M.; Hedegaard, G. B.

    2009-04-01

    In Denmark, where birch pollen is considered to be among the most important allergenic pollen, about one million people suffer from seasonal allergic rhinitis. In Denmark, the official reported pollen forecast is based on the daily weather forecast, the pollen calendar and local 24-h measurements. Birch pollen has the potential for long-range transport but the present Danish pollen forecast does not account for birch pollen being transported into the country from distant sources.. Long-range transport episodes are intermittent and often out of the main pollen season, where individuals in general will be medically unprotected. Here we use an integrated approach to investigate whether or not Denmark receives significant quantities of birch pollen from Poland and Germany before local trees start to flower. In 2006 we used a combination of phenological observations and pollen measurements in Poland (Poznań) and Denmark (Copenhagen). Seasonal and diurnal variations in birch pollen measurement from Copenhagen (2000-2006) were examined with the aim of identifying pre-seasonal episodes originating from long-range transport. The 2.5% accumulation method was used for identifying start of season. If daily pollen counts exceeded 30 grains/m3 either before the local flowering season began or on the actual start day, the episode was chosen for investigation with back trajectory analysis. A birch forest inventory for Northern Europe was produced and implemented in DEHM-Pollen along with a simple unified pollen release model SUPREME to investigate the 2006 campaign in detail. In 2006, full flowering took place in Poznan between 20th and 28th of April and daily concentrations varied between 739 and 2169 grains/m3. In Copenhagen phenological observations showed that local flowering was initiated the 2nd of May. In Copenhagen several episodes with pollen concentrations at 108, 244 and 41 grains/m3 were recorded the 23rd, 26th and 27th of April, respectively. Back-trajectory analysis

  14. Spatiotemporal models for predicting high pollen concentration level of Corylus, Alnus, and Betula

    NASA Astrophysics Data System (ADS)

    Nowosad, Jakub

    2016-06-01

    Corylus, Alnus, and Betula trees are among the most important sources of allergic pollen in the temperate zone of the Northern Hemisphere and have a large impact on the quality of life and productivity of allergy sufferers. Therefore, it is important to predict high pollen concentrations, both in time and space. The aim of this study was to create and evaluate spatiotemporal models for predicting high Corylus, Alnus, and Betula pollen concentration levels, based on gridded meteorological data. Aerobiological monitoring was carried out in 11 cities in Poland and gathered, depending on the site, between 2 and 16 years of measurements. According to the first allergy symptoms during exposure, a high pollen count level was established for each taxon. An optimizing probability threshold technique was used for mitigation of the problem of imbalance in the pollen concentration levels. For each taxon, the model was built using a random forest method. The study revealed the possibility of moderately reliable prediction of Corylus and highly reliable prediction of Alnus and Betula high pollen concentration levels, using preprocessed gridded meteorological data. Cumulative growing degree days and potential evaporation proved to be two of the most important predictor variables in the models. The final models predicted not only for single locations but also for continuous areas. Furthermore, the proposed modeling framework could be used to predict high pollen concentrations of Corylus, Alnus, Betula, and other taxa, and in other countries.

  15. Pollen Analysis of Natural Honeys from the Central Region of Shanxi, North China

    PubMed Central

    Song, Xiao-Yan; Yao, Yi-Feng; Yang, Wu-De

    2012-01-01

    Based on qualitative and quantitative melissopalynological analyses, 19 Chinese honeys were classified by botanical origin to determine their floral sources. The honey samples were collected during 2010–2011 from the central region of Shanxi Province, North China. A diverse spectrum of 61 pollen types from 37 families was identified. Fourteen samples were classified as unifloral, whereas the remaining samples were multifloral. Bee-favoured families (occurring in more than 50% of the samples) included Caprifoliaceae (found in 10 samples), Laminaceae (10), Brassicaceae (12), Rosaceae (12), Moraceae (13), Rhamnaceae (15), Asteraceae (17), and Fabaceae (19). In the unifloral honeys, the predominant pollen types were Ziziphus jujuba (in 5 samples), Robinia pseudoacacia (3), Vitex negundo var. heterophylla (2), Sophora japonica (1), Ailanthus altissima (1), Asteraceae type (1), and Fabaceae type (1). The absolute pollen count (i.e., the number of pollen grains per 10 g honey sample) suggested that 13 samples belonged to Group I (<20,000 pollen grains), 4 to Group II (20,000–100,000), and 2 to Group III (100,000–500,000). The dominance of unifloral honeys without toxic pollen grains and the low value of the HDE/P ratio (i.e., honey dew elements/pollen grains from nectariferous plants) indicated that the honey samples are of good quality and suitable for human consumption. PMID:23185358

  16. The long distance transport (LDT) of Ambrosia pollen from the Pannonian Plain to Scandinavia

    NASA Astrophysics Data System (ADS)

    Šikoparija, B..; Skjøth, C. A.; Alm Kübler, K.; Dahl, A.; Radišić, P.; Sommer, J.; Grewling, Ł.; Smith, M.

    2012-04-01

    Ragweed (Ambrosia spp.) pollen grains are important aeroallergens that cause seasonal allergic rhinitis and asthma to sensitive individuals. This study describes the conditions required for the LDT of ragweed pollen from the Pannonian Plain (PP) to Sweden on the 27- 28 August 2011, using a combination of daily and bi-hourly pollen count data, the overall synoptic weather situation, 3D analysis of the regional scale orography using Digital Elevation Models, surface meteorological data, satellite observations, and air mas trajectories calculated using the HYSPLIT model. During the episode, high pressure (1024-1028 hPa) situated over European Russia and the Black Sea to the east and deep low pressure (~990 hPa) over the British Isles in the northwest resulted in a general southeast-northwest movement of air, and the occurrence of the jet-effect Kosava wind in the PP. This dry and gusty wind caused ragweed pollen release on the PP and pollen to be transported to the northwest. A foehn wind that governs air movement down leeward slopes into the PP was also active. The 24 and 25 August 2011 were very hot and caused large amounts of ragweed pollen to be released and taken high up in the atmosphere through convection. Such conditions also resulted in high Planetary Boundary Layers over the entire area, conditions that facilitated the transport of pollen over areas of low elevation on the Western Carpathians (i.e. the Moravian Gate or Low Baskid passes) northward into Poland and beyond.

  17. Spatiotemporal models for predicting high pollen concentration level of Corylus, Alnus, and Betula.

    PubMed

    Nowosad, Jakub

    2016-06-01

    Corylus, Alnus, and Betula trees are among the most important sources of allergic pollen in the temperate zone of the Northern Hemisphere and have a large impact on the quality of life and productivity of allergy sufferers. Therefore, it is important to predict high pollen concentrations, both in time and space. The aim of this study was to create and evaluate spatiotemporal models for predicting high Corylus, Alnus, and Betula pollen concentration levels, based on gridded meteorological data. Aerobiological monitoring was carried out in 11 cities in Poland and gathered, depending on the site, between 2 and 16 years of measurements. According to the first allergy symptoms during exposure, a high pollen count level was established for each taxon. An optimizing probability threshold technique was used for mitigation of the problem of imbalance in the pollen concentration levels. For each taxon, the model was built using a random forest method. The study revealed the possibility of moderately reliable prediction of Corylus and highly reliable prediction of Alnus and Betula high pollen concentration levels, using preprocessed gridded meteorological data. Cumulative growing degree days and potential evaporation proved to be two of the most important predictor variables in the models. The final models predicted not only for single locations but also for continuous areas. Furthermore, the proposed modeling framework could be used to predict high pollen concentrations of Corylus, Alnus, Betula, and other taxa, and in other countries. PMID:26487352

  18. [Study of the coverage of pollen capture in Lyon over three seasons (1999, 2000, 2001)].

    PubMed

    Thibaudon, M; Burnichon, A; Deruaz, D; Laurent, O

    2002-05-01

    For the third consecutive year, the two sensors of the type HIRST of the National Network of aerobiology monitoring (RNSA) worked on the agglomeration of Lyon. The primary trap (Lyon 1) is located at 26 m height on a roof in southern zone of Lyon (district of Gerland), the second (Lyon 2) is located in northern zone of the city (district of Vaise) on a roof at 15 m height compared to the ground. The study of the daily variations of the pollinic counts over the first two years had shown a perfect parallelism for pollens of trees, Poaceae and Urticaceae. Only the curves of pollens of ambrosia presented different layouts between the two pollen traps. The study of this third year makes it possible to consolidate the proceeding results and to appreciate the value of the cover of a Hirst pollen trap in urban implantation within the framework of the allergo-pollinic monitoring. PMID:12108330

  19. Pollen mixing in pollen generalist solitary bees: a possible strategy to complement or mitigate unfavourable pollen properties?

    PubMed

    Eckhardt, Michael; Haider, Mare; Dorn, Silvia; Müller, Andreas

    2014-05-01

    Generalist herbivorous insects, which feed on plant tissue that is nutritionally heterogeneous or varies in its content of secondary metabolites, often benefit from dietary mixing through more balanced nutrient intake or reduced exposure to harmful secondary metabolites. Pollen is similarly heterogeneous as other plant tissue in its content of primary and secondary metabolites, suggesting that providing their offspring with mixed pollen diets might be a promising strategy for pollen generalist bees to complement nutrient imbalances or to mitigate harmful secondary metabolites of unfavourable pollen. In the present study, we compared larval performance of the pollen generalist solitary bee species Osmia cornuta (Megachilidae) on five experimental pollen diets that consisted of different proportions of unfavourable pollen diet of Ranunculus acris (Ranunculaceae) and favourable pollen diet of Sinapis arvensis (Brassicaceae). In addition, we microscopically analysed the pollen contained in the scopal brushes of field-collected females of O. cornuta and three closely related species to elucidate to what degree these pollen generalist bees mix pollen of different hosts in their brood cells. In striking contrast to a pure Ranunculus pollen diet, which had a lethal effect on most developing larvae of O. cornuta, larval survival, larval development time and adult body mass of both males and females remained nearly unaffected by the admixture of up to 50% of Ranunculus pollen diet to the larval food. Between 42% and 66% of all female scopal pollen loads analysed contained mixtures of pollen from two to six plant families, indicating that pollen mixing is a common behaviour in O. cornuta and the three related bee species. The present study provides the first evidence that the larvae of pollen generalist bees can benefit from the nutrient content of unfavourable pollen without being negatively affected by its unfavourable chemical properties if such pollen is mixed with

  20. Knockin' on pollen's door: live cell imaging of early polarization events in germinating Arabidopsis pollen

    PubMed Central

    Vogler, Frank; Konrad, Sebastian S. A.; Sprunck, Stefanie

    2015-01-01

    Pollen tubes are an excellent system for studying the cellular dynamics and complex signaling pathways that coordinate polarized tip growth. Although several signaling mechanisms acting in the tip-growing pollen tube have been described, our knowledge on the subcellular and molecular events during pollen germination and growth site selection at the pollen plasma membrane is rather scarce. To simultaneously track germinating pollen from up to 12 genetically different plants we developed an inexpensive and easy mounting technique, suitable for every standard microscope setup. We performed high magnification live-cell imaging during Arabidopsis pollen activation, germination, and the establishment of pollen tube tip growth by using fluorescent marker lines labeling either the pollen cytoplasm, vesicles, the actin cytoskeleton or the sperm cell nuclei and membranes. Our studies revealed distinctive vesicle and F-actin polarization during pollen activation and characteristic growth kinetics during pollen germination and pollen tube formation. Initially, the germinating Arabidopsis pollen tube grows slowly and forms a uniform roundish bulge, followed by a transition phase with vesicles heavily accumulating at the growth site before switching to rapid tip growth. Furthermore, we found the two sperm cells to be transported into the pollen tube after the phase of rapid tip growth has been initiated. The method presented here is suitable to quantitatively study subcellular events during Arabidopsis pollen germination and growth, and for the detailed analysis of pollen mutants with respect to pollen polarization, bulging, or growth site selection at the pollen plasma membrane. PMID:25954283

  1. The influence from synoptic weather on the variation of air pollution and pollen exposure

    NASA Astrophysics Data System (ADS)

    Grundström, Maria; Dahl, Åslög; Chen, Deliang; Pleijel, Håkan

    2014-05-01

    Exposure to elevated air pollution levels can make people more susceptible to allergies or result in more severe allergic reactions for people with an already pronounced sensitivity to pollen. The aim of this study was to investigate the relationships between urban air pollution (nitrogen oxides, ozone and particles) and airborne Betula pollen in Gothenburg, Sweden, during the pollen seasons for the years 2001-2012. Further, the influence from atmospheric weather pattern on pollen/pollution related risk, using Lamb Weather Types (LWT), was also considered. Daily LWTs were obtained by comparing the variation in atmospheric pressure from a 16 point grid over a given region on earth (scale ~1000km) and essentially describe the air mass movement for the region. They include two non-directional types, cyclonic (C) and anticyclonic (A) and eight directional types depending on the wind direction (N, NE, E... etc.). LWTs with dry and calm meteorological character e.g. limited precipitation and low to moderate wind speeds (A, NE, E, SE) were associated with strongly elevated air pollution and pollen levels where Betula was exceptionally high in LWTs NE and E. The co-variation between Betula pollen and ozone was strong and significant during situations with LWTs A, NE, E and SE. The most important conclusion from this study was that LWTs A, NE, E and SE were associated with high pollen and air pollution levels and can therefore be classified as high risk weather situations for combined air pollution and pollen exposure. Our study shows that LWTs have the potential to be developed into an objective tool for integrated air quality forecasting and a warning system for risk of high exposure situations.

  2. Near-surface and columnar measurements with a micro pulse lidar of atmospheric pollen in Barcelona, Spain

    NASA Astrophysics Data System (ADS)

    Sicard, Michaël; Izquierdo, Rebeca; Alarcón, Marta; Belmonte, Jordina; Comerón, Adolfo; Baldasano, José Maria

    2016-06-01

    We present for the first time continuous hourly measurements of pollen near-surface concentration and lidar-derived profiles of particle backscatter coefficients and of volume and particle depolarization ratios during a 5-day pollination event observed in Barcelona, Spain, between 27 and 31 March 2015. Daily average concentrations ranged from 1082 to 2830 pollen m-3. Platanus and Pinus pollen types represented together more than 80 % of the total pollen. Maximum hourly pollen concentrations of 4700 and 1200 m-3 were found for Platanus and Pinus, respectively. Every day a clear diurnal cycle caused by the vertical transport of the airborne pollen was visible on the lidar-derived profiles with maxima usually reached between 12:00 and 15:00 UT. A method based on the lidar polarization capabilities was used to retrieve the contribution of the pollen to the total aerosol optical depth (AOD). On average the diurnal (09:00-17:00 UT) pollen AOD was 0.05, which represented 29 % of the total AOD. Maximum values of the pollen AOD and its contribution to the total AOD reached 0.12 and 78 %, respectively. The diurnal means of the volume and particle depolarization ratios in the pollen plume were 0.08 and 0.14, with hourly maxima of 0.18 and 0.33, respectively. The diurnal mean of the height of the pollen plume was found at 1.24 km with maxima varying in the range of 1.47-1.78 km. A correlation study is performed (1) between the depolarization ratios and the pollen near-surface concentration to evaluate the ability of the former parameter to monitor pollen release and (2) between the depolarization ratios as well as pollen AOD and surface downward solar fluxes, which cause the atmospheric turbulences responsible for the particle vertical motion, to examine the dependency of the depolarization ratios and the pollen AOD upon solar fluxes. For the volume depolarization ratio the first correlation study yields to correlation coefficients ranging 0.00-0.81 and the second to

  3. Cleanroom Design Practices and Their Influence on Particle Counts

    NASA Technical Reports Server (NTRS)

    Hogue, Patrick

    2008-01-01

    This paper will discuss the adverse effects of deficient cleanroom design practices on airborne particle counts and the rather curious correlation of particle count variations with external environmental pressure fluctuations. Data is also presented that demonstrates that APL building 23 cleanrooms ran well below ISO class 7 (FED class 10,000) during New Horizons and STEREO integration.

  4. Insect visitation and pollen deposition in an invaded prairie plant community

    USGS Publications Warehouse

    Larson, D.L.; Royer, R.A.; Royer, M.R.

    2006-01-01

    Invasive plants with large flowering displays have been shown to compete with native plants for pollinator services, often to the detriment of native plant fitness. In this study, we compare the pollinator communities and pollen deposited on stigmas of native plant species within and away from stands of the invasive alien plant, leafy spurge (Euphorbia esula) at a large natural area in North Dakota, USA. Specifically, we ask if infestation influences (1) visitation rates and taxonomic composition of visitors to native flowers, and (2) the amount of conspecific pollen, number of pollen species, and proportion of heterospecific pollen on stigmas of native plants. We observed visits to selected native species during May and June 2000 and 2001. Stigmas were collected from a subsample of the flowers within these plots, squashed, and the pollen identified and counted under a light microscope. Visitation varied between years and among species of native plants: infestation had mixed effects in 2000 but visitation, especially by halictids was always lower within infestations in 2001. Despite differences in visitation between years, we found significantly less conspecific pollen on stigmas from infested plots in six of eight cases; we never found significantly more conspecific pollen on stigmas from within infestations. Our results emphasize the temporal variability in plant-pollinator relations and the added complexity imposed by an invasive species that will always make prediction of effects difficult. Nonetheless, the consistently lower conspecific pollen counts on native stigmas within infestations, regardless of visitation, suggest the likelihood of negative effects. ?? 2005 Elsevier Ltd. All rights reserved.

  5. The role of oak pollen in hay fever consultations in general practice and the factors influencing patients' decisions to consult.

    PubMed Central

    Ross, A M; Corden, J M; Fleming, D M

    1996-01-01

    BACKGROUND: Patients often consult for hay fever before significant counts of grass pollen are recorded, and this has prompted the question, 'Are symptoms already present or are patients consulting to obtain medication in anticipation?' AIM: The study is concerned with the relationship between hay fever symptoms and pollens, and also with the impact of the media on patient consulting behaviour. METHOD: Symptom questionnaires were presented to patients consulting with hay fever for the first time that year in 1994 in four Birmingham practices. The questionnaire concerned the nature and duration of symptoms and the influence of the media on their decision to consult. Incidence data collected over the spring and summer periods (1989-1995) in the Weekly Returns Service (WRS) were examined in relation to pollen counts reported by the Midlands Asthma and Allergy Research Association at Derby. Data are presented for oak, birch and grass pollen, but other pollen data including rape, nettle and other trees were also examined. RESULTS: Questionnaire data from 1994 were analysed in two periods starting from 4 April: early (day 1-60) and late (day 61-124). Out of the 364 subjects, 38% consulted in the early period and 62% in the late period. Altogether, 41% developed symptoms before the start of the grass pollen season. Overall, 91% of patients first consulting in the early period had already experienced symptoms compared with 99% late period and were not simply collecting prescriptions in anticipation. The influence of the media on consultation behaviour was very small, except in children, 23% of whom (or their parents) were reported to be influenced. The new episode data from the WRS examined over 7 years showed an early peak that was coterminous with oak pollen, and a later and higher peak with grass pollen. CONCLUSION: The consistency of the relationship between oak pollen and the early peak of hay fever over the years examined suggests that oak pollen is a major cause of

  6. Bioassaying for ozone with pollen systems

    SciTech Connect

    Feder, W.A.

    1981-01-01

    Sensitivity to ozone of pollen germinating in vitro is closely correlated with ozone sensitivity of the pollen parent. Ozone-sensitive and tolerant pollen populations have been identified in tobacco, petunia, and tomato cultivars. The rate of tube elongation can be reversibly slowed or stopped by exposure to low concentrations of ozone. The performance of selected pollen populations can then be used to bioassay ozone in ambient air by introducing the air sample into a growth chamber where ozone-sensitive pollen in growing. Year-round pollen producion can be achieved in the greenhouse. Harvested pollen can be tested, packaged, and transported to user facilities without loss of vigor. Pollen populations are inexpensive to produce, respond reliably, and are simple to use as a bioassay for air quality.

  7. Identification and persistence of Pinus pollen DNA on cotton fabrics: A forensic application.

    PubMed

    Schield, Cassandra; Campelli, Cassandra; Sycalik, Jennifer; Randle, Christopher; Hughes-Stamm, Sheree; Gangitano, David

    2016-01-01

    Advances in plant genomics have had an impact on the field of forensic botany. However, the use of pollen DNA profiling in forensic investigations has yet to be applied. Five volunteers wore a jacket with Pinus echinata pollen-containing cotton swatches for a 14-day period. Pollen decay was evaluated at days 0, 3, 6, 9 and 14 by microscopy. Pollen grains were then transferred to slides using a portable forensic vacuum handle. Ten single grains per swatch were isolated for DNA analysis. DNA was extracted using a high throughput extraction method. A nine-locus short tandem repeat (STR) multiplex system, including previously published primers from Pinus taeda, was developed. DNA was amplified by PCR using fluorescent dyes and analyzed by capillary electrophoresis. Pollen counts from cotton swatches in a 14-day period exhibited an exponential decay from 100% to 17%. The success rate of PCR amplification was 81.2%. Complete and partial STR profiles were generated from 250 pollen grains analyzed (44% and 37%, respectively). Due to the limited amount of DNA, drop-in events were observed (1.87%). However, the rate of contamination with pollen from other pine individuals originating from environmental sources was 4.4%. In conclusion, this study has shown that pollen can be a stable source of forensic DNA evidence, as a proof-of-principle, and that may persist on cotton clothing for at least 14 days of wear. This method can be applied in forensic cases where pollen grains larger than 10 μm (e.g., from herbs or trees) may be transferred to clothing (worn by suspect or victim) by primary contact. PMID:26746823

  8. Pollen loads of eucalypt and other pollen types in birds in NW Spain.

    PubMed

    Calviño-Cancela, María; Neumann, Max

    2015-12-01

    Here we present the amount of pollen of eucalypt and pollen of other types for birds captured in two bird ringing stations for 14 months (March 2014 to April 2015) in NW Spain. Common and latin names of all birds species captured, together with the number of captured individuals (N), prevalence of eucalypt pollen (percentage of individuals with eucalypt pollen) and of pollen of other types and average pollen loads per individual for eucalypt and other pollen types is presented. See [1] for further information and discussion. PMID:26568978

  9. Pollen loads of eucalypt and other pollen types in birds in NW Spain

    PubMed Central

    Calviño-Cancela, María; Neumann, Max

    2015-01-01

    Here we present the amount of pollen of eucalypt and pollen of other types for birds captured in two bird ringing stations for 14 months (March 2014 to April 2015) in NW Spain. Common and latin names of all birds species captured, together with the number of captured individuals (N), prevalence of eucalypt pollen (percentage of individuals with eucalypt pollen) and of pollen of other types and average pollen loads per individual for eucalypt and other pollen types is presented. See [1] for further information and discussion. PMID:26568978

  10. [The epidemiology of pollen allergy].

    PubMed

    Charpin, D; Caillaud, D

    2014-04-01

    The prevalence of seasonal allergic rhinitis can be established through surveys performed in a sample of the general population. These surveys are based on a questionnaire, which could lead to an overestimate of prevalence rates, and on measurements of specific IgE, which need to be interpreted in the light of the responses to the questionnaire. Such surveys are few in France and need to be updated. Risk factors for seasonal allergic rhinitis are genetic, epigenetic and environmental. Relationships between exposure to pollen and health can be documented through ecological and panel surveys. Panel surveys may give information on threshold levels and dose-response relationships. In addition to pollen exposure, global warming and air pollutants act as cofactors. Monitoring of both pollen exposure and its health effects should be encouraged and strengthened. PMID:24750956

  11. Pollen Allergens for Molecular Diagnosis.

    PubMed

    Pablos, Isabel; Wildner, Sabrina; Asam, Claudia; Wallner, Michael; Gadermaier, Gabriele

    2016-04-01

    Pollen allergens are one of the main causes of type I allergies affecting up to 30 % of the population in industrialized countries. Climatic changes affect the duration and intensity of pollen seasons and may together with pollution contribute to increased incidences of respiratory allergy and asthma. Allergenic grasses, trees, and weeds often present similar habitats and flowering periods compromising clinical anamnesis. Molecule-based approaches enable distinction between genuine sensitization and clinically mostly irrelevant IgE cross-reactivity due to, e. g., panallergens or carbohydrate determinants. In addition, sensitivity as well as specificity can be improved and lead to identification of the primary sensitizing source which is particularly beneficial regarding polysensitized patients. This review gives an overview on relevant pollen allergens and their usefulness in daily practice. Appropriate allergy diagnosis is directly influencing decisions for therapeutic interventions, and thus, reliable biomarkers are pivotal when considering allergen immunotherapy in the context of precision medicine. PMID:27002515

  12. Visual Recognition Software for Binary Classification and its Application to Pollen Identification

    NASA Astrophysics Data System (ADS)

    Punyasena, S. W.; Tcheng, D. K.; Nayak, A.

    2014-12-01

    An underappreciated source of uncertainty in paleoecology is the uncertainty of palynological identifications. The confidence of any given identification is not regularly reported in published results, so cannot be incorporated into subsequent meta-analyses. Automated identifications systems potentially provide a means of objectively measuring the confidence of a given count or single identification, as well as a mechanism for increasing sample sizes and throughput. We developed the software ARLO (Automated Recognition with Layered Optimization) to tackle difficult visual classification problems such as pollen identification. ARLO applies pattern recognition and machine learning to the analysis of pollen images. The features that the system discovers are not the traditional features of pollen morphology. Instead, general purpose image features, such as pixel lines and grids of different dimensions, size, spacing, and resolution, are used. ARLO adapts to a given problem by searching for the most effective combination of feature representation and learning strategy. We present a two phase approach which uses our machine learning process to first segment pollen grains from the background and then classify pollen pixels and report species ratios. We conducted two separate experiments that utilized two distinct sets of algorithms and optimization procedures. The first analysis focused on reconstructing black and white spruce pollen ratios, training and testing our classification model at the slide level. This allowed us to directly compare our automated counts and expert counts to slides of known spruce ratios. Our second analysis focused on maximizing classification accuracy at the individual pollen grain level. Instead of predicting ratios of given slides, we predicted the species represented in a given image window. The resulting analysis was more scalable, as we were able to adapt the most efficient parts of the methodology from our first analysis. ARLO was able to

  13. Pollen Recovery from Insects: Light Microscopy

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Numerous insect species feed on the pollen, nectar, and other plant exudates that are associated with flowers. As a result of this feeding activity, pollen becomes attached to the insects. Analysis of the pollen attached to these insects can reveal what insects eat, their dispersal patterns in and...

  14. [Pollen content of the Paris air: comparison of the results obtained by two samplers for the year 2003].

    PubMed

    Distigny, C; Polenne, J P; Bordenave, L; Bex, V; Squinazi, F

    2004-09-01

    During the 2003 season, a second pollen collector has been established in Paris city (Audubon site) to study the representativeness of the initial collector situated on the roof of the Pasteur Institute. The Hygiene Laboratory of Paris followed the pollen counts from the two collectors, during the period going from May to September. Both the samplers are Lanzoni model with a flow rate of 10 l x min(-1). The quantitative results show that no statistical difference exists between the pollen counts obtained from the 2 sites (Mann-Withney test, p > .05). The dominant species are Urticaceae, Poacae and Castenea species for the 2 collectors. Their counts are similar except for Poacae whose results are higher at the Audubon site. The collection period do not take into account the pollen production period of many trees species because of the late installation of the collector on the Audubon site. The results show that the two sites chosen for the study of pollen distribution are comparable. Nevertheless, the comparative study should be maintained on the next year to get more details about the observed differences and to collect the early trees pollen. PMID:15529829

  15. BURSTING POLLEN is required to organize the pollen germination plaque and pollen tube tip in Arabidopsis thaliana.

    PubMed

    Hoedemaekers, Karin; Derksen, Jan; Hoogstrate, Suzanne W; Wolters-Arts, Mieke; Oh, Sung-Aeong; Twell, David; Mariani, Celestina; Rieu, Ivo

    2015-04-01

    Pollen germination may occur via the so-called germination pores or directly through the pollen wall at the site of contact with the stigma. In this study, we addressed what processes take place during pollen hydration (i.e. before tube emergence), in a species with extra-poral pollen germination, Arabidopsis thaliana. A T-DNA mutant population was screened by segregation distortion analysis. Histological and electron microscopy techniques were applied to examine the wild-type and mutant phenotypes. Within 1 h of the start of pollen hydration, an intine-like structure consisting of cellulose, callose and at least partly de-esterified pectin was formed at the pollen wall. Subsequently, this 'germination plaque' gradually extended and opened up to provide passage for the cytoplasm into the emerging pollen tube. BURSTING POLLEN (BUP) was identified as a gene essential for the correct organization of this plaque and the tip of the pollen tube. BUP encodes a novel Golgi-located glycosyltransferase related to the glycosyltransferase 4 (GT4) subfamily which is conserved throughout the plant kingdom. Extra-poral pollen germination involves the development of a germination plaque and BUP defines the correct plastic-elastic properties of this plaque and the pollen tube tip by affecting pectin synthesis or delivery. PMID:25442716

  16. Use of Remote Sensing and Dust Modelling to Evaluate Ecosystem Phenology and Pollen Dispersal

    NASA Technical Reports Server (NTRS)

    Luvall, Jeffrey C.; Sprigg, William A.; Watts, Carol; Shaw, Patrick

    2007-01-01

    The impact of pollen release and downwind concentrations can be evaluated utilizing remote sensing. Previous NASA studies have addressed airborne dust prediction systems PHAiRS (Public Health Applications in Remote Sensing) which have determined that pollen forecasts and simulations are possible. By adapting the deterministic dust model (as an in-line system with the National Weather Service operational forecast model) used in PHAiRS to simulate downwind dispersal of pollen, initializing the model with pollen source regions from MODIS, assessing the results a rapid prototype concept can be produced. We will present the results of our effort to develop a deterministic model for predicting and simulating pollen emission and downwind concentration to study details or phenology and meteorology and their dependencies, and the promise of a credible real time forecast system to support public health and agricultural science and service. Previous studies have been done with PHAiRS research, the use of NASA data, the dust model and the PHAiRS potential to improve public health and environmental services long into the future.

  17. Plants remember past weather: a study for atmospheric pollen concentrations of Ambrosia, Poaceae and Populus

    NASA Astrophysics Data System (ADS)

    Matyasovszky, István; Makra, László; Csépe, Zoltán; Sümeghy, Zoltán; Deák, Áron József; Pál-Molnár, Elemér; Tusnády, Gábor

    2015-10-01

    After extreme dry (wet) summers or years, pollen production of different taxa may decrease (increase) substantially. Accordingly, studying effects of current and past meteorological conditions on current pollen concentrations for different taxa have of major importance. The purpose of this study is separating the weight of current and past weather conditions influencing current pollen productions of three taxa. Two procedures, namely multiple correlations and factor analysis with special transformation are used. The 11-year (1997-2007) data sets include daily pollen counts of Ambrosia (ragweed), Poaceae (grasses) and Populus (poplar), as well as daily values of four climate variables (temperature, relative humidity, global solar flux and precipitation). Multiple correlations of daily pollen counts with simultaneous values of daily meteorological variables do not show annual course for Ambrosia, but do show definite trends for Populus and Poaceae. Results received using the two methods revealed characteristic similarities. For all the three taxa, the continental rainfall peak and additional local showers in the growing season can strengthen the weight of the current meteorological elements. However, due to the precipitation, big amount of water can be stored in the soil contributing to the effect of the past climate elements during dry periods. Higher climate sensitivity (especially water sensitivity) of the herbaceous taxa ( Ambrosia and Poaceae) can be definitely established compared to the arboreal Populus. Separation of the weight of the current and past weather conditions for different taxa involves practical importance both for health care and agricultural production.

  18. The influence of temperature and climate change on the timing of pollen release in the Netherlands

    NASA Astrophysics Data System (ADS)

    van Vliet, Arnold J. H.; Overeem, Aart; de Groot, Rudolf S.; Jacobs, Adrie F. G.; Spieksma, Frits T. M.

    2002-11-01

    In the last decade it has become clear that the timing of many phenological processes, like the start of flowering and leaf unfolding in spring, have changed. The increase in temperature is believed to be the main cause. The earlier start of flowering will have consequences for the start of the pollen season, and thus for the start of the hay fever season. Millions of people world-wide will therefore experience the impact of climate change in their daily lives during spring and summer. In this paper we analyse the relation between climate parameters, especially temperature, and the start of the pollen season in the western part of the Netherlands based on daily pollen counts of the Leiden University Medical Centre and temperature measurements from 1969 till 2000 by the Royal Netherlands Meteorological Institute in De Bilt. The results indicate that there is a strong correlation between temperature and start of the pollen season. An advance of the start of the pollen season of 3 to 22 days has been observed. The potential future changes in the start of the pollen season under climate change scenarios are also discussed.

  19. Cytochemical localization of some hydrolases in the pollen and pollen tubes of Amaryllis vittata Ait.

    PubMed

    Sharma, D

    1982-01-01

    Some hydrolases are localized cytochemically in the pollen and pollen tubes of Amaryllis vittata Ait. The function of different enzymes is discussed in relation to pollen tubes morphogenesis. Activity of most of the enzymes was confined to colpus region, pollen wall and general cytoplasm of pollen and pollen tube. The activity of hydrolytic enzymes like acid monophosphoesterase and lipase and was nil in the exine of both germinated and ungerminated pollen, whereas intense reaction for esterase was observed in exine. Enzyme activity increased after germination which suggest the hydrolysis of stored metabolites and synthesis of proteins and other metabolites for the active growth of pollen tube. Intense reaction for enzymes like alkaline phosphomonoesterase, ATP-ase, 5-nucleotidase etc. at the tip region of pollen tube suggest their role in physiological processes associated with exchange of materials through intercellular transport during tube wall polysaccharide biogenesis. PMID:6298081

  20. [Development of allergic reactivity to Artemesia pollen during combined sensitization to pollen and microbes].

    PubMed

    Ermekova, R K

    1978-08-01

    Some regularities of formation of hypersensitivity of the immediate type to the pollen of Artemisia absinthium were studied under conditions of combined hypersensitivity to pollen and Brucella abortus 19-BA vaccine strain; the latter was administered 3, 12, and 28 days after the pollen. The degree of specific allergic reconstruction to the pollen was studied by passive skin anaphylaxis after Ovary, indirect degranulation of mast cells of healthy rats, and by general anaphylaxis in response to intravenous injection of the Artemisia absinthium pollen water-salt extract. Early formation of allergy to the pollen was observed in the groups of animals with combined hypersensitivity to the pollen and brucellae. The degree of allergic reactivity to the pollen allergen was more expressed in the groups with combined allergy than in those with pure pollen hypersensitivity at all the stages of this experiment. PMID:99195

  1. Pollen selection under acid rain stress

    SciTech Connect

    Zhang, Y.

    1994-01-01

    To investigate whether acid rain stress induces pollen selection in nature, three different approaches were used, based on the assumption that the response of pollen grains to acid rain is controlled by an acid sensitive gene product. Germination of pollen from homozygous and heterozygous individuals under acid rain stress was examined to detect any differences in rate of germination between populations of homogeneous and heterogeneous pollen grains. In vitro and in vivo bulked segregant analysis using RAPDs was used to search for differences in DNA constitution between the survivors of acid rain stressed and non-acid rain stressed pollen populations in vitro and between the progenies of acid rain stressed and non-acid rain stressed populations during pollination, respectively. No evidence for the pollen selection under acid rain stress was obtained in any of the test systems. Inhibition of protein synthesis using cycloheximide led to significant reduction of tube elongation at 4 hr and had no effect on pollen germination at any time interval tested. Total proteins extracted from control and acid rain stressed pollen grain populations exhibited no differences. The reduction of corn pollen germination in vitro under acid rain stress was mainly due to pollen rupture. The present data indicates the reduction of pollen germination and tube growth under acid rain stress may be a physiological response rather than a genetic response. A simple, nontoxic, and effective method to separate germinated from ungerminated pollen grains has been developed using pollen from corn (Zea mays, L. cv. Pioneer 3747). The separated germinated pollen grains retained viability and continued tube growth when placed in culture medium.

  2. Linking pollinator visitation rate and pollen receipt.

    PubMed

    Cayenne Engel, E; Irwin, Rebecca E

    2003-11-01

    The majority of flowering plants require animals for pollination, a critical ecosystem service in natural and agricultural systems. However, quantifying useful estimates of pollinator visitation rates can be nearly impossible when pollinator visitation is infrequent. We examined the utility of an indirect measure of pollinator visitation, namely pollen receipt by flowers, using the hummingbird-pollinated plant, Ipomopsis aggregata (Polemoniaceae). Our a priori hypothesis was that increased pollinator visitation should result in increased pollen receipt by stigmas. However, the relationship between pollinator visitation rate and pollen receipt may be misleading if pollen receipt is a function of both the number of pollinator visits and variation in pollinator efficiency at depositing pollen, especially in the context of variable floral morphology. Therefore, we measured floral and plant characters known to be important to pollinator visitation and/or pollen receipt in I. aggregata (corolla length and width and plant height) and used path analysis to dissect and compare the effect of pollinator visitation rate vs. pollinator efficiency on pollen receipt. Of the characters we measured, pollinator visitation rate (number of times plants were visited multiplied by the mean percentage of flowers probed per visit) had the strongest direct positive effect on pollen receipt, explaining 36% of the variation in pollen receipt. Plant height had a direct positive effect on pollinator visitation rate and an indirect positive effect on pollen receipt. Despite the supposition that floral characters would directly affect pollen receipt as a result of changes in pollinator efficiency, corolla length and width only weakly affected pollen receipt. These results suggest a direct positive link between pollinator visitation rate and pollen receipt across naturally varying floral morphology in I. aggregata. Understanding the relationship between pollinator visitation rate and pollen

  3. Effect of natural grass pollen exposure on exhaled nitric oxide in asthmatic children.

    PubMed

    Baraldi, E; Carrá, S; Dario, C; Azzolin, N; Ongaro, R; Marcer, G; Zacchello, F

    1999-01-01

    Exhaled nitiric oxide (NO) is increased in exhaled breath of asthmatic patients. The aim of this study was to investigate the longitudinal changes of exhaled NO outside and during the pollen season in pollen-allergic asthmatic children. Twenty-one children (age 6 to 16 yr), with a seasonal allergic asthma sensitive to grass pollen, underwent measurements of exhaled NO and pulmonary function before (March), during (May), and after (November) the pollen season. Exhaled NO was measured by a tidal breathing method with a chemiluminescence analyzer and NO steady-state levels were recorded. The timing of the measurements during the pollen season was based on the atmospheric pollen count. Exhaled NO values of asthmatic children were compared with those of 21 sex- and age-matched healthy children. Pulmonary function and symptoms of asthma were also evaluated at each visit. The mean value of exhaled NO before the grass season was 12.7 +/- 5.1 ppb (mean +/- SD), significantly higher when compared with controls (7.8 +/- 2.7 ppb, p < 0.001). In the pollen season there was a significant (p < 0.001) twofold increase in exhaled NO (21.4 +/- 7.6 ppb) that, after the season, returned to values similar (12.8 +/- 5.8 ppb, p = NS) to those found before the season. There were no significant changes in FEV1 before and during the season (98.6% predicted versus 101% predicted, p = NS). We conclude that natural allergen exposure is related to an increase of exhaled NO in asthmatic grass pollen-allergic children even in absence of significant changes in airways function. We speculate that measurement of exhaled NO could be a sensitive noninvasive marker of asthma disease activity. PMID:9872848

  4. Acute asthma epidemics, weather and pollen in England, 1987-1994.

    PubMed

    Newson, R; Strachan, D; Archibald, E; Emberlin, J; Hardaker, P; Collier, C

    1998-03-01

    Recent epidemics of acute asthma have caused speculation that, if their causes were known, early warnings might be feasible. In particular, some epidemics seemed to be associated with thunderstorms. We wondered what risk factors predicting epidemics could be identified. Daily asthma admissions counts during 1987-1994, for two age groups (0-14 yrs and > or = 15 yrs), were measured using the Hospital Episodes System (HES). Epidemics were defined as combinations of date, age group and English Regional Health Authority (RHA) with exceptionally high asthma admission counts compared to the predictions of a log-linear autoregression model. They were compared with control days 1 week before and afterwards, regarding seven meteorological variables and 5 day average pollen counts for four species. Fifty six asthma epidemics were identified. The mean density of sferics (lightning flashes), temperature and rainfall on epidemic days were greater than those on control days. High sferics densities were overrepresented in epidemics. Simultaneously high sferics and grass pollen further increased the probability of an epidemic, but only to 15% (95% confidence interval 2-45%). Two thirds of epidemics were not preceded by thunderstorms. Thunderstorms and high grass pollen levels precede asthma epidemics more often than expected by chance. However, most epidemics are not associated with thunderstorms or unusual weather conditions, and most thunderstorms, even following high grass pollen levels, do not precede epidemics. An early warning system based on the indicators examined here would, therefore, detect few epidemics and generate an unacceptably high rate of false alarms. PMID:9596123

  5. Forecasting daily pollen concentrations using data-driven modeling methods in Thessaloniki, Greece

    NASA Astrophysics Data System (ADS)

    Voukantsis, Dimitris; Niska, Harri; Karatzas, Kostas; Riga, Marina; Damialis, Athanasios; Vokou, Despoina

    2010-12-01

    Airborne pollen have been associated with allergic symptoms in sensitized individuals, having a direct impact on the overall quality of life of a considerable fraction of the population. Therefore, forecasting elevated airborne pollen concentrations and communicating this piece of information to the public are key issues in prophylaxis and safeguarding the quality of life of the overall population. In this study, we adopt a data-oriented approach in order to develop operational forecasting models (1-7 days ahead) of daily average airborne pollen concentrations of the highly allergenic taxa: Poaceae, Oleaceae and Urticaceae. The models are developed using a representative dataset consisting of pollen and meteorological time-series recorded during the years 1987-2002, in the city of Thessaloniki, Greece. The input variables (features) of the models have been optimized by making use of genetic algorithms, whereas we evaluate the performance of three algorithms: i) multi-Layer Perceptron, ii) support vector regression and iii) regression trees originating from distinct domains of Computational Intelligence (CI), and compare the resulting models with traditional multiple linear regression models. Results show the superiority of CI methods, especially when forecasting several days ahead, compared to traditional multiple linear regression models. Furthermore, the CI models complement each other, resulting to a combined model that performs better than each one separately. The overall performance ranges, in terms of the index of agreement, from 0.85 to 0.93 clearly suggesting the potential operational use of the models. The latter ones can be utilized in provision of personalized and on-time information services, which can improve quality of life of sensitized citizens.

  6. Pollen-Associated Microbiome Correlates with Pollution Parameters and the Allergenicity of Pollen.

    PubMed

    Obersteiner, Andrea; Gilles, Stefanie; Frank, Ulrike; Beck, Isabelle; Häring, Franziska; Ernst, Dietrich; Rothballer, Michael; Hartmann, Anton; Traidl-Hoffmann, Claudia; Schmid, Michael

    2016-01-01

    Pollen allergies have been rapidly increasing over the last decades. Many allergenic proteins and non-allergenic adjuvant compounds of pollen are involved in the plant defense against environmental or microbial stress. The first aim of this study was to analyze and compare the colonizing microbes on allergenic pollen. The second aim was to investigate detectable correlations between pollen microbiota and parameters of air pollution or pollen allergenicity. To reach these aims, bacterial and fungal DNA was isolated from pollen samples of timothy grass (Phleum pratense, n = 20) and birch trees (Betula pendula, n = 55). With this isolated DNA, a terminal restriction fragment length polymorphism analysis was performed. One result was that the microbial diversity on birch tree and timothy grass pollen samples (Shannon/Simpson diversity indices) was partly significantly correlated to allergenicity parameters (Bet v 1/Phl p 5, pollen-associated lipid mediators). Furthermore, the microbial diversity on birch pollen samples was correlated to on-site air pollution (nitrogen dioxide (NO2), ammonia (NH3), and ozone (O3)). What is more, a significant negative correlation was observed between the microbial diversity on birch pollen and the measured NO2 concentrations on the corresponding trees. Our results showed that the microbial composition of pollen was correlated to environmental exposure parameters alongside with a differential expression of allergen and pollen-associated lipid mediators. This might translate into altered allergenicity of pollen due to environmental and microbial stress. PMID:26910418

  7. Pollen-Associated Microbiome Correlates with Pollution Parameters and the Allergenicity of Pollen

    PubMed Central

    Obersteiner, Andrea; Gilles, Stefanie; Frank, Ulrike; Beck, Isabelle; Häring, Franziska; Ernst, Dietrich; Rothballer, Michael; Hartmann, Anton; Traidl-Hoffmann, Claudia; Schmid, Michael

    2016-01-01

    Pollen allergies have been rapidly increasing over the last decades. Many allergenic proteins and non-allergenic adjuvant compounds of pollen are involved in the plant defense against environmental or microbial stress. The first aim of this study was to analyze and compare the colonizing microbes on allergenic pollen. The second aim was to investigate detectable correlations between pollen microbiota and parameters of air pollution or pollen allergenicity. To reach these aims, bacterial and fungal DNA was isolated from pollen samples of timothy grass (Phleum pratense, n = 20) and birch trees (Betula pendula, n = 55). With this isolated DNA, a terminal restriction fragment length polymorphism analysis was performed. One result was that the microbial diversity on birch tree and timothy grass pollen samples (Shannon/Simpson diversity indices) was partly significantly correlated to allergenicity parameters (Bet v 1/Phl p 5, pollen-associated lipid mediators). Furthermore, the microbial diversity on birch pollen samples was correlated to on-site air pollution (nitrogen dioxide (NO2), ammonia (NH3), and ozone (O3)). What is more, a significant negative correlation was observed between the microbial diversity on birch pollen and the measured NO2 concentrations on the corresponding trees. Our results showed that the microbial composition of pollen was correlated to environmental exposure parameters alongside with a differential expression of allergen and pollen-associated lipid mediators. This might translate into altered allergenicity of pollen due to environmental and microbial stress. PMID:26910418

  8. Mineral content of commercial pollen.

    PubMed

    Orzáez Villanueva, M T; Díaz Marquina, A; Bravo Serrano, R; Blaźquez Abellán, G

    2001-05-01

    Pollen is a natural product which is extending its marketing day by day, given that it is considered to be a dietetic product and it is consumed everyday by a broad sector of the Spanish population. In its composition it presents valuable nutrients, among which we can find minerals, which is the main object of this study. We have analysed sodium, potassium, magnesium, copper, iron, manganese and zinc in 15 pollen samples which correspond to different brands. The technique we have used is atomic absortion spectroscopy. The results show us the great potassium contribution of this natural product, with values over 400 mg/100 g, and about microelements, mainly iron and zinc, although with different results, depending on the brand which markets it, with average values of 4.01 +/- 1.00 and 3.66 +/- 1.02, respectively. PMID:11400473

  9. Eosinophil count - absolute

    MedlinePlus

    Eosinophils; Absolute eosinophil count ... the white blood cell count to give the absolute eosinophil count. ... than 500 cells per microliter (cells/mcL). Normal value ranges may vary slightly among different laboratories. Talk ...

  10. Detection of chaos: New approach to atmospheric pollen time-series analysis

    NASA Astrophysics Data System (ADS)

    Bianchi, M. M.; Arizmendi, C. M.; Sanchez, J. R.

    1992-09-01

    Pollen and spores are biological particles that are ubiquitous to the atmosphere and are pathologically significant, causing plant diseases and inhalant allergies. One of the main objectives of aerobiological surveys is forecasting. Prediction models are required in order to apply aerobiological knowledge to medical or agricultural practice; a necessary condition of these models is not to be chaotic. The existence of chaos is detected through the analysis of a time series. The time series comprises hourly counts of atmospheric pollen grains obtained using a Burkard spore trap from 1987 to 1989 at Mar del Plata. Abraham's method to obtain the correlation dimension was applied. A low and fractal dimension shows chaotic dynamics. The predictability of models for atomspheric pollen forecasting is discussed.

  11. Environmental pollen trapped by tobacco leaf as indicators of the provenance of counterfeit cigarette products: a preliminary investigation and test of concept.

    PubMed

    Donaldson, Margaret P; Stephens, William E

    2010-05-01

    The global trade in counterfeit tobacco products is increasingly taking market share from legal brands in many parts of the developed world, with attendant adverse economic, health, criminal, and other societal impacts. Knowing the geographical source is central to developing new strategies for curbing this illicit trade, and here, the potential of environmental pollen extracted from manufactured cigarettes is examined. Two samples representing U.S. and Chinese brands were investigated for their pollen content. Results indicate that tobacco leaf very efficiently captures environmental pollen (about 1800 and 12,600 grains per cigarette, respectively) with no detectable self-contamination by the tobacco plant. In both cases, the flora is typical of open space environments, but pollen type counts indicate very different distributions of species. This preliminary investigation indicates that palynology has the potential to constrain geographical source(s) of tobacco, particularly if regionally localized species can be recognized among the pollen. PMID:20202071

  12. Airborne oceanographic lidar system

    NASA Technical Reports Server (NTRS)

    Bressel, C.; Itzkan, I.; Nunes, J. E.; Hoge, F.

    1977-01-01

    The characteristics of an Airborne Oceanographic Lidar (AOL) are given. The AOL system is described and its potential for various measurement applications including bathymetry and fluorosensing is discussed.

  13. Evaluating multiplexed next-generation sequencing as a method in palynology for mixed pollen samples.

    PubMed

    Keller, A; Danner, N; Grimmer, G; Ankenbrand, M; von der Ohe, K; von der Ohe, W; Rost, S; Härtel, S; Steffan-Dewenter, I

    2015-03-01

    The identification of pollen plays an important role in ecology, palaeo-climatology, honey quality control and other areas. Currently, expert knowledge and reference collections are essential to identify pollen origin through light microscopy. Pollen identification through molecular sequencing and DNA barcoding has been proposed as an alternative approach, but the assessment of mixed pollen samples originating from multiple plant species is still a tedious and error-prone task. Next-generation sequencing has been proposed to avoid this hindrance. In this study we assessed mixed pollen probes through next-generation sequencing of amplicons from the highly variable, species-specific internal transcribed spacer 2 region of nuclear ribosomal DNA. Further, we developed a bioinformatic workflow to analyse these high-throughput data with a newly created reference database. To evaluate the feasibility, we compared results from classical identification based on light microscopy from the same samples with our sequencing results. We assessed in total 16 mixed pollen samples, 14 originated from honeybee colonies and two from solitary bee nests. The sequencing technique resulted in higher taxon richness (deeper assignments and more identified taxa) compared to light microscopy. Abundance estimations from sequencing data were significantly correlated with counted abundances through light microscopy. Simulation analyses of taxon specificity and sensitivity indicate that 96% of taxa present in the database are correctly identifiable at the genus level and 70% at the species level. Next-generation sequencing thus presents a useful and efficient workflow to identify pollen at the genus and species level without requiring specialised palynological expert knowledge. PMID:25270225

  14. Pollen-limited reproduction in blue oak: Implications for wind pollination in fragmented populations

    USGS Publications Warehouse

    Knapp, E.E.; Goedde, M.A.; Rice, K.J.

    2001-01-01

    counted in radii ranging from 30 m to 80 m. The association between number of pollen-producing neighbors and acorn production was strongest when neighborhood sizes of 60 m or larger were considered. Our results suggest that fragmentation and thinning of blue oak woodlands may reduce pollen availability and limit reproduction in this wind-pollinated species.

  15. Specialized bees fail to develop on non-host pollen: do plants chemically protect their pollen?

    PubMed

    Praz, Christophe J; Müller, Andreas; Dorn, Silvia

    2008-03-01

    Bees require large amounts of pollen for their own reproduction. While several morphological flower traits are known to have evolved to protect plants against excessive pollen harvesting by bees, little is known on how selection to minimize pollen loss acts on the chemical composition of pollen. In this study, we traced the larval development of four solitary bee species, each specialized on a different pollen source, when reared on non-host pollen by transferring unhatched eggs of one species onto the pollen provisions of another species. Pollen diets of Asteraceae and Ranunculus (Ranunculaceae) proved to be inadequate for all bee species tested except those specialized on these plants. Further, pollen of Sinapis (Brassicaceae) and Echium (Boraginaceae) failed to support larval development in one bee species specialized on Campanula (Campanulaceae). Our results strongly suggest that pollen of these four taxonomic groups possess protective properties that hamper digestion and thus challenge the general view of pollen as an easy-to-use protein source for flower visitors. PMID:18459342

  16. Evaluation of three portable samplers for monitoring airborne fungi

    NASA Technical Reports Server (NTRS)

    Mehta, S. K.; Mishra, S. K.; Pierson, D. L.

    1996-01-01

    Airborne fungi were monitored at five sample sites with the Burkard portable, the RCS Plus, and the SAS Super 90 air samplers; the Andersen 2-stage impactor was used for comparison. All samplers were calibrated before being used simultaneously to collect 100-liter samples at each site. The Andersen and Burkard samplers retrieved equivalent volumes of airborne fungi; the SAS Super 90 and RCS Plus measurements did not differ from each other but were significantly lower than those obtained with the Andersen or Burkard samplers. Total fungal counts correlated linearly with Cladosporium and Penicillium counts. Alternaria species, although present at all sites, did not correlate with total count or with amounts of any other fungal genera. Sampler and location significantly influenced fungal counts, but no interactions between samplers and locations were found.

  17. A Review of the Effects of Major Atmospheric Pollutants on Pollen Grains, Pollen Content, and Allergenicity

    PubMed Central

    Sénéchal, Hélène; Visez, Nicolas; Charpin, Denis; Shahali, Youcef; Peltre, Gabriel; Biolley, Jean-Philippe; Lhuissier, Franck; Couderc, Rémy; Yamada, Ohri; Malrat-Domenge, Audrey; Pham-Thi, Nhân; Poncet, Pascal; Sutra, Jean-Pierre

    2015-01-01

    This review summarizes the available data related to the effects of air pollution on pollen grains from different plant species. Several studies carried out either on in situ harvested pollen or on pollen exposed in different places more or less polluted are presented and discussed. The different experimental procedures used to monitor the impact of pollution on pollen grains and on various produced external or internal subparticles are listed. Physicochemical and biological effects of artificial pollution (gaseous and particulate) on pollen from different plants, in different laboratory conditions, are considered. The effects of polluted pollen grains, subparticles, and derived aeroallergens in animal models, in in vitro cell culture, on healthy human and allergic patients are described. Combined effects of atmospheric pollutants and pollen grains-derived biological material on allergic population are specifically discussed. Within the notion of “polluen,” some methodological biases are underlined and research tracks in this field are proposed. PMID:26819967

  18. The pollen tube paradigm revisited.

    PubMed

    Kroeger, Jens; Geitmann, Anja

    2012-12-01

    The polar growth process characterizing pollen tube elongation has attracted numerous modeling attempts over the past years. While initial models focused on recreating the correct cellular geometry, recent models are increasingly based on experimentally assessed cellular parameters such as the dynamics of signaling processes and the mechanical properties of the cell wall. Recent modeling attempts have therefore substantially gained in biological relevance and predictive power. Different modeling methods are explained and the power and limitations of individual models are compared. Focus is on several recent models that use closed feedback loops in order to generate limit cycles representing the oscillatory behavior observed in growing tubes. PMID:23000432

  19. Polyamines in Pollen: From Microsporogenesis to Fertilization

    PubMed Central

    Aloisi, Iris; Cai, Giampiero; Serafini-Fracassini, Donatella; Del Duca, Stefano

    2016-01-01

    The entire pollen life span is driven by polyamine (PA) homeostasis, achieved through fine regulation of their biosynthesis, oxidation, conjugation, compartmentalization, uptake, and release. The critical role of PAs, from microsporogenesis to pollen–pistil interaction during fertilization, is suggested by high and dynamic transcript levels of PA biosynthetic genes, as well as by the activities of the corresponding enzymes. Moreover, exogenous supply of PAs strongly affects pollen maturation and pollen tube elongation. A reduction of endogenous free PAs impacts pollen viability both in the early stages of pollen development and during fertilization. A number of studies have demonstrated that PAs largely function by modulating transcription, by structuring pollen cell wall, by modulating protein (mainly cytoskeletal) assembly as well as by modulating the level of reactive oxygen species. Both free low-molecular weight aliphatic PAs, and PAs conjugated to proteins and hydroxyl-cinnamic acids take part in these complex processes. Here, we review both historical and recent evidence regarding molecular events underlying the role of PAs during pollen development. In the concluding remarks, the outstanding issues and directions for future research that will further clarify our understanding of PA involvement during pollen life are outlined. PMID:26925074

  20. Antioxidant Activity of Sonoran Desert Bee Pollen

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bee products have been consumed by mankind since antiquity and their health benefits are becoming more apparent. Bee pollen (pollen collected by honey bees) was collected in the high intensity ultraviolet (UV) Sonoran desert and was analyzed by the anti-2,2-diphenyl-1-picryhydrazyl (DPPH) assay and...

  1. Occupational contact urticaria and late-phase bronchial asthma caused by compositae pollen in a florist.

    PubMed

    Uter, W; Nöhle, M; Randerath, B; Schwanitz, H J

    2001-09-01

    Insect-pollinated members of the plant family Compositae (Asteraceae) rarely cause immediate-type hypersensitivity disease; however, this may have quite disabling consequences, which is shown by the case of a 42-year-old female florist. She developed contact urticaria later accompanied by rhinoconjunctivitis and bronchial asthma with maximum obstruction occurring some hours after the end of occupational exposure to the causative Compositae pollens of, for example, dandelions, blazing star, golden rod, yarrow, Aster ssp, chrysanthemums, and marguerite. Skin testing revealed immediate-type hypersensitivity to several members of the above-mentioned plant family confirmed by demonstration of specific IgE antibodies. Bronchial provocation testing yielded a positive response with all 4 pollen extracts tested. The patient had to give up work in a flower shop, because sufficient avoidance of airborne inhalant exposure was not considered practical. PMID:11526527

  2. New insights into ragweed pollen allergens.

    PubMed

    Bordas-Le Floch, Véronique; Groeme, Rachel; Chabre, Henri; Baron-Bodo, Véronique; Nony, Emmanuel; Mascarell, Laurent; Moingeon, Philippe

    2015-11-01

    Pollen allergens from short ragweed (Ambrosia artemisiifolia) cause severe respiratory allergies in North America and Europe. To date, ten short ragweed pollen allergens belonging to eight protein families, including the recently discovered novel major allergen Amb a 11, have been recorded in the International Union of Immunological Societies (IUIS) allergen database. With evidence that other components may further contribute to short ragweed pollen allergenicity, a better understanding of the allergen repertoire is a requisite for the design of proper diagnostic tools and efficient immunotherapies. This review provides an update on both known as well as novel candidate allergens from short ragweed pollen, identified through a comprehensive characterization of the ragweed pollen transcriptome and proteome. PMID:26383916

  3. Preservation of cycad and Ginkgo pollen

    USGS Publications Warehouse

    Frederiksen, N.O.

    1978-01-01

    Pollen grains of Ginkgo, Cycas, and Encephalartos were chemically treated together with pollen of Quercus, Alnus, and Pinus, the latter three genera being used as standards. The experiments showed that: (1) boiling the pollen for 8-10 hours in 10% KOH had little if any effect on any of the grains; (2) lengthy acetolysis treatment produced some degradation or corrosion, particularly in Ginkgo and Cycas, but the grains of even these genera remained easily recognizable; (3) oxidation with KMnO4 followed by H2O2 showed that pollen of Ginkgo, Cycas, and Encephalartos remains better preserved than that of Quercus and Alnus, and although Ginkgo and Encephalartos probably are slightly less resistant to oxidation than Pinus, no great differences exists between these monosulcate types and Pinus. Thus the experiments show that, at least for sediments low in bacteria, cycad and Ginkgo pollen should be well represented in the fossil record as far as their preservational capabilities are concerned. ?? 1978.

  4. Pollen dispersal by Artemisia tridentata (Asteraceae)

    NASA Astrophysics Data System (ADS)

    Laursen, S. C.; Reiners, W. A.; Kelly, R. D.; Gerow, K. G.

    2007-08-01

    While the biophysics of anemophilous pollen dispersal is understood in principle, empirical studies for testing such principles are rare, particularly in native ecosystems. This paper describes mechanisms underlying the dispersal of Artemisia pollen in a Wyoming sagebrush steppe. The relationships between meteorological variables and pollen flux were defined during the 1999 Artemisia flowering season, and detailed processes at the individual plant level were experimentally tested in the field in 2000. Results indicated that Artemisia pollen presentation is continuous but with early morning maxima. Atmospheric pollen concentrations and potential dispersal rates are controlled at diurnal time scales by individual flower development together with characteristic changes in temperature/humidity and wind speeds, at multi-day scales by frontal weather patterns, and at week-long scales by flowering phenology.

  5. Immersion freezing of birch pollen washing water

    NASA Astrophysics Data System (ADS)

    Augustin, S.; Wex, H.; Niedermeier, D.; Pummer, B.; Grothe, H.; Hartmann, S.; Tomsche, L.; Clauss, T.; Voigtländer, J.; Ignatius, K.; Stratmann, F.

    2013-11-01

    Birch pollen grains are known to be ice nucleating active biological particles. The ice nucleating activity has previously been tracked down to biological macromolecules that can be easily extracted from the pollen grains in water. In the present study, we investigated the immersion freezing behavior of these ice nucleating active (INA) macromolecules. Therefore we measured the frozen fractions of particles generated from birch pollen washing water as a function of temperature at the Leipzig Aerosol Cloud Interaction Simulator (LACIS). Two different birch pollen samples were considered, with one originating from Sweden and one from the Czech Republic. For the Czech and Swedish birch pollen samples, freezing was observed to start at -19 and -17 °C, respectively. The fraction of frozen droplets increased for both samples down to -24 °C. Further cooling did not increase the frozen fractions any more. Instead, a plateau formed at frozen fractions below 1. This fact could be used to determine the amount of INA macromolecules in the droplets examined here, which in turn allowed for the determination of nucleation rates for single INA macromolecules. The main differences between the Swedish birch pollen and the Czech birch pollen were obvious in the temperature range between -17 and -24 °C. In this range, a second plateau region could be seen for Swedish birch pollen. As we assume INA macromolecules to be the reason for the ice nucleation, we concluded that birch pollen is able to produce at least two different types of INA macromolecules. We were able to derive parameterizations for the heterogeneous nucleation rates for both INA macromolecule types, using two different methods: a simple exponential fit and the Soccer ball model. With these parameterization methods we were able to describe the ice nucleation behavior of single INA macromolecules from both the Czech and the Swedish birch pollen.

  6. Pollen grains for oral vaccination

    PubMed Central

    Atwe, Shashwati U.; Ma, Yunzhe; Gill, Harvinder Singh

    2015-01-01

    Oral vaccination can offer a painless and convenient method of vaccination. Furthermore, in addition to systemic immunity it has potential to stimulate mucosal immunity through antigen-processing by the gut-associated lymphoid tissues. In this study we propose the concept that pollen grains can be engineered for use as a simple modular system for oral vaccination. We demonstrate feasibility of this concept by using spores of Lycopodium clavatum (clubmoss) (LSs). We show that LSs can be chemically cleaned to remove native proteins to create intact clean hollow LS shells. Empty pollen shells were successfully filled with molecules of different sizes demonstrating their potential to be broadly applicable as a vaccination system. Using ovalbumin (OVA) as a model antigen, LSs formulated with OVA were orally fed to mice. LSs stimulated significantly higher anti-OVA serum IgG and fecal IgA antibodies compared to those induced by use of cholera toxin as a positive-control adjuvant. The antibody response was not affected by pre-neutralization of the stomach acid, and persisted for up to seven months. Confocal microscopy revealed that LSs can translocate in to mouse intestinal wall. Overall, this study lays the foundation of using LSs as a novel approach for oral vaccination. PMID:25151980

  7. Pollen grains for oral vaccination.

    PubMed

    Atwe, Shashwati U; Ma, Yunzhe; Gill, Harvinder Singh

    2014-11-28

    Oral vaccination can offer a painless and convenient method of vaccination. Furthermore, in addition to systemic immunity it has potential to stimulate mucosal immunity through antigen-processing by the gut-associated lymphoid tissues. In this study we propose the concept that pollen grains can be engineered for use as a simple modular system for oral vaccination. We demonstrate feasibility of this concept by using spores of Lycopodium clavatum (clubmoss) (LSs). We show that LSs can be chemically cleaned to remove native proteins to create intact clean hollow LS shells. Empty pollen shells were successfully filled with molecules of different sizes demonstrating their potential to be broadly applicable as a vaccination system. Using ovalbumin (OVA) as a model antigen, LSs formulated with OVA were orally fed to mice. LSs stimulated significantly higher anti-OVA serum IgG and fecal IgA antibodies compared to those induced by use of cholera toxin as a positive-control adjuvant. The antibody response was not affected by pre-neutralization of the stomach acid, and persisted for up to 7 months. Confocal microscopy revealed that LSs can translocate into mouse intestinal wall. Overall, this study lays the foundation of using LSs as a novel approach for oral vaccination. PMID:25151980

  8. Pollen limitation and Allee effect related to population size and sex ratio in the endangered Ottelia acuminata (Hydrocharitaceae): implications for conservation and reintroduction.

    PubMed

    Xia, J; Lu, J; Wang, Z X; Hao, B B; Wang, H B; Liu, G H

    2013-03-01

    Small populations may suffer more severe pollen limitation and result in Allee effects. Sex ratio may also affect pollination and reproduction success in dioecious species, which is always overlooked when performing conservation and reintroduction tasks. In this study, we investigated whether and how population size and sex ratio affected pollen limitation and reproduction in the endangered Ottelia acuminata, a dioecious submerged species. We established experimental plots with increasing population size and male sex ratio. We observed insect visitation, estimated pollen limitation by hand-pollinations and counted fruit set and seed production per fruit. Fruit set and seed production decreased significantly in small populations due to pollinator scarcity and thus suffered more severe pollen limitation. Although frequently visited, female-biased larger populations also suffered severe pollen limitation due to few effective visits and insufficient pollen availability. Rising male ratio enhanced pollination service and hence reproduction. Unexpectedly, pollinator preferences did not cause reduced reproduction in male-biased populations because of high pollen availability. However, reproductive outputs showed more variability in severe male-biased populations. Our results revealed two component Allee effects in fruit set and seed production, mediated by pollen limitation in O. acuminata. Moreover, reproduction decreased significantly in larger female-biased populations, increasing the risk of an Allee effect. PMID:22963276

  9. Measurement of the amount and number of pollen particles of Cryptomeria japonica (taxodiaceae) by imaging with a photoacoustic microscope.

    PubMed

    Miyamoto, Katsuhiko; Hoshimiya, Tsutomu

    2006-03-01

    A photoacoustic microscope (PAM), which includes a condenser microphone and a pair of linear-motor-driven pulse stages, was specially designed for spectroscopic applications. The PAM was applied to measure the amount and number of pollen particles of Cryptomeria japonica (CJ), which is known for its allergic function against eyes and nose. The advantage of photoacoustic (PA) imaging is both its high sensitivity and its counting ability up to high concentrations of the specimen. The CJ pollen particles were fixed on a piece of adhesive tape or on albumen (egg white) on a glass slide set in a PA cell. The PA image showed the ability of this method to count CJ pollen from the several-hundred-milligram region to even a single particle. The PA signal obtained was integrated over the specimen surface. The dependence of the PA signal on the amount or number of the pollen particles was measured. The resulting coefficients of correlation of the calibration curves for the amount and the number of pollen particles were 0.94 and 0.97, respectively. PMID:16555767

  10. An evidential example of airborne bacteria in a crowded, underground public concourse in Tokyo

    NASA Astrophysics Data System (ADS)

    Seino, Kaoruko; Takano, Takehito; Nakamura, Keiko; Watanabe, Masafumi

    2005-01-01

    We examined airborne bacteria in an underground concourse in Tokyo and investigated conditions that influenced bacterial counts. Airborne bacteria were collected by using an impactor sampler. Colonies on plate count agar (PCA) and Columbia colistin-nalidixic acid agar with 5% sheep blood (CNA agar) were enumerated. The range, geometric mean, and 95% CI of the bacterial counts (CFU m-3) on PCA and CNA agar were 150-1380, 456, 382-550 and 50-990, 237, 182-309, respectively. Bacterial counts on PCA significantly correlated with number of the pedestrians (r=0.89), relative humidity (r=0.70) and airborne dust (PM5.0) (r=0.73). Results of a multiple regression indicated independent positive association between the number of pedestrians and bacterial counts on PCA (p<0.01) after excluding the influence of relative humidity and airborne dust. Similar results were obtained with the statistical analysis for the counts of bacteria on CNA agar. Gram-positive cocci were dominant on PCA and CNA agar. Staphylococcus epidermidis and Micrococcus spp. were dominant among the 11 genera and 19 species identified in the present study. Considering the pattern of identified species and the significant independent association between number of pedestrians and bacterial counts, airborne bacteria in a crowded underground concourse were mostly originated from the pedestrians who were walking in the underground concourse. This study gave an evidential example of bacterial conditions in the air of an underground crowded public space in Tokyo.

  11. Pollen

    MedlinePlus

    ... most common grasses that can cause allergies are: Bermuda grass Johnson grass Kentucky bluegrass Orchard grass Sweet ... Health Sciences 111 T.W. Alexander Drive Research Triangle Park, N.C. 27709 Last Reviewed: July 14, ...

  12. Quantifying Aerial Concentrations of Maize Pollen in the Atmospheric Surface Layer Using Remote-Piloted Airplanes and Lagrangian Stochastic Modeling

    NASA Astrophysics Data System (ADS)

    Aylor, Donald E.; Boehm, Matthew T.; Shields, Elson J.

    2006-07-01

    The extensive adoption of genetically modified crops has led to a need to understand better the dispersal of pollen in the atmosphere because of the potential for unwanted movement of genetic traits via pollen flow in the environment. The aerial dispersal of maize pollen was studied by comparing the results of a Lagrangian stochastic (LS) model with pollen concentration measurements made over cornfields using a combination of tower-based rotorod samplers and airborne radio-controlled remote-piloted vehicles (RPVs) outfitted with remotely operated pollen samplers. The comparison between model and measurements was conducted in two steps. In the first step, the LS model was used in combination with the rotorod samplers to estimate the pollen release rate Q for each sampling period. In the second step, a modeled value for the concentration Cmodel, corresponding to each RPV measured value Cmeasure, was calculated by simulating the RPV flight path through the LS model pollen plume corresponding to the atmospheric conditions, field geometry, wind direction, and source strength. The geometric mean and geometric standard deviation of the ratio Cmodel/Cmeasure over all of the sampling periods, except those determined to be upwind of the field, were 1.42 and 4.53, respectively, and the lognormal distribution corresponding to these values was found to fit closely the PDF of Cmodel/Cmeasure. Model output was sensitive to the turbulence parameters, with a factor-of-100 difference in the average value of Cmodel over the range of values encountered during the experiment. In comparison with this large potential variability, it is concluded that the average factor of 1.4 between Cmodel and Cmeasure found here indicates that the LS model is capable of accurately predicting, on average, concentrations over a range of atmospheric conditions.

  13. Isolation of total RNA from pollens.

    PubMed

    Bijli, K M; Singh, B P; Sridhara, S; Arora, N

    2001-05-01

    Isolation of total RNA from plant materials has been difficult, due to the presence of complex organic substances and the associated pigmentation. In fact, there is a dearth of standardized protocols for isolating total RNA from pollens. To find a simple and reliable method for isolating total RNA from pollen, four methods, viz. phenol/SDS (PS), guanidine HCl (GH), tri-reagent (TR), and modified SDS-betaME (SB) were tested with fresh pollen of Ricinus communis (procured at -70 degrees C) and pollen dried at 30-37 degrees C. The quality and quantity of RNA was superior for the material processed at -70 degrees C. SB gave the highest RNA yield (2.35 mg/g, OD260/280 >2.0), compared to other methods. The results obtained by the SB method were found to be comparable with the widely used tri-reagent method. This was validated with other pollens of Imperata cylindrica and Xanthium strumarium. The yield obtained from graded amounts of pollen was consistent with SB, compared to the TR method. The RNA isolated by SB gave good quality mRNA for synthesizing cDNA. The SDS-betaME method is simple, efficient, and uses less expensive reagents. Hence, we recommend the modified SDS-betaME method for isolating total RNA from pollens. PMID:11426703

  14. Thunderstorm-asthma and pollen allergy.

    PubMed

    D'Amato, G; Liccardi, G; Frenguelli, G

    2007-01-01

    Thunderstorms have been linked to asthma epidemics, especially during the pollen seasons, and there are descriptions of asthma outbreaks associated with thunderstorms, which occurred in several cities, prevalently in Europe (Birmingham and London in the UK and Napoli in Italy) and Australia (Melbourne and Wagga Wagga). Pollen grains can be carried by thunderstorm at ground level, where pollen rupture would be increased with release of allergenic biological aerosols of paucimicronic size, derived from the cytoplasm and which can penetrate deep into lower airways. In other words, there is evidence that under wet conditions or during thunderstorms, pollen grains may, after rupture by osmotic shock, release into the atmosphere part of their content, including respirable, allergen-carrying cytoplasmic starch granules (0.5-2.5 microm) or other paucimicronic components that can reach lower airways inducing asthma reactions in pollinosis patients. The thunderstorm-asthma outbreaks are characterized, at the beginning of thunderstorms by a rapid increase of visits for asthma in general practitioner or hospital emergency departments. Subjects without asthma symptoms, but affected by seasonal rhinitis can experience an asthma attack. No unusual levels of air pollution were noted at the time of the epidemics, but there was a strong association with high atmospheric concentrations of pollen grains such as grasses or other allergenic plant species. However, subjects affected by pollen allergy should be informed about a possible risk of asthma attack at the beginning of a thunderstorm during pollen season. PMID:17156336

  15. Airborne Laser Polar Nephelometer

    NASA Technical Reports Server (NTRS)

    Grams, Gerald W.

    1973-01-01

    A polar nephelometer has been developed at NCAR to measure the angular variation of the intensity of light scattered by air molecules and particles. The system has been designed for airborne measurements using outside air ducted through a 5-cm diameter airflow tube; the sample volume is that which is common to the intersection of a collimated source beam and the detector field of view within the airflow tube. The source is a linearly polarized helium-neon laser beam. The optical system defines a collimated field-of-view (0.5deg half-angle) through a series of diaphragms located behind a I72-mm focal length objective lens. A photomultiplier tube is located immediately behind an aperture in the focal plane of the objective lens. The laser beam is mechanically chopped (on-off) at a rate of 5 Hz; a two-channel pulse counter, synchronized to the laser output, measures the photomultiplier pulse rate with the light beam both on and off. The difference in these measured pulse rates is directly proportional to the intensity of the scattered light from the volume common to the intersection of the laser beam and the detector field-of-view. Measurements can be made at scattering angles from 15deg to 165deg with reference to the direction of propagation of the light beam. Intermediate angles are obtained by selecting the angular increments desired between these extreme angles (any multiple of 0.1deg can be selected for the angular increment; 5deg is used in normal operation). Pulses provided by digital circuits control a stepping motor which sequentially rotates the detector by pre-selected angular increments. The synchronous photon-counting system automatically begins measurement of the scattered-light intensity immediately after the rotation to a new angle has been completed. The instrument has been flown on the NASA Convair 990 airborne laboratory to obtain data on the complex index of refraction of atmospheric aerosols. A particle impaction device is operated simultaneously

  16. Airborne gravity is here

    SciTech Connect

    Hammer, S.

    1982-01-11

    After 20 years of development efforts, the airborne gravity survey has finally become a practical exploration method. Besides gravity data, the airborne survey can also collect simultaneous, continuous records of high-precision magneticfield data as well as terrain clearance; these provide a topographic contour map useful in calculating terrain conditions and in subsequent planning and engineering. Compared with a seismic survey, the airborne gravity method can cover the same area much more quickly and cheaply; a seismograph could then detail the interesting spots.

  17. The Big Pumpkin Count.

    ERIC Educational Resources Information Center

    Coplestone-Loomis, Lenny

    1981-01-01

    Pumpkin seeds are counted after students convert pumpkins to jack-o-lanterns. Among the activities involved, pupils learn to count by 10s, make estimates, and to construct a visual representation of 1,000. (MP)

  18. Pollen sequence at Kirchner Marsh, Minnesota

    USGS Publications Warehouse

    Winter, T.C.

    1962-01-01

    A pollen diagram from Kirchner Marsh, southeastern Minnesota, records a continuous vegetation sequence from the time of Late Wisconsin ice retreat from the region. The late-glacial and early postglacial portions of the diagram are correlated with a radiocarbon-dated diagram from Madelia, Minnesota. Both diagrams show a series of maxima of pollen types in the early postglacial that suggest a significant climatic change at that time. The Kirchner diagram, in addition, shows high percentages of nonarboreal pollen later in the postglacial that indicate an advance of prairie elements into the area between 7200 and 5000 years ago.

  19. Bee Pollen: Chemical Composition and Therapeutic Application

    PubMed Central

    Komosinska-Vassev, Katarzyna; Olczyk, Pawel; Kaźmierczak, Justyna; Olczyk, Krystyna

    2015-01-01

    Bee pollen is a valuable apitherapeutic product greatly appreciated by the natural medicine because of its potential medical and nutritional applications. It demonstrates a series of actions such as antifungal, antimicrobial, antiviral, anti-inflammatory, hepatoprotective, anticancer immunostimulating, and local analgesic. Its radical scavenging potential has also been reported. Beneficial properties of bee pollen and the validity for their therapeutic use in various pathological condition have been discussed in this study and with the currently known mechanisms, by which bee pollen modulates burn wound healing process. PMID:25861358

  20. Intracellular auxin transport in pollen

    PubMed Central

    Dal Bosco, Cristina; Dovzhenko, Alexander; Palme, Klaus

    2012-01-01

    Cellular auxin homeostasis is controlled at many levels that include auxin biosynthesis, auxin metabolism, and auxin transport. In addition to intercellular auxin transport, auxin homeostasis is modulated by auxin flow through the endoplasmic reticulum (ER). PIN5, a member of the auxin efflux facilitators PIN protein family, was the first protein to be characterized as an intracellular auxin transporter. We demonstrated that PIN8, the closest member of the PIN family to PIN5, represents another ER-residing auxin transporter. PIN8 is specifically expressed in the male gametophyte and is located in the ER. By combining genetic, physiological, cellular and biochemical data we demonstrated a role for PIN8 in intracellular auxin homeostasis. Although our investigation shed light on intracellular auxin transport in pollen, the physiological function of PIN8 still remains to be elucidated. Here we discuss our data taking in consideration other recent findings. PMID:22990451

  1. In children allergic to ragweed pollen, nasal inflammation is not influenced by monosensitization or polysensitization

    PubMed Central

    Gelardi, Matteo; Bosoni, Mariangela; Morelli, Marco; Beretta, Silvia; Incorvaia, Cristoforo; Buttafava, Serena; Landi, Massimo; Masieri, Simonetta; Frati, Franco; Quaranta, Nicola; Zuccotti, Gian Vincenzo

    2016-01-01

    Background In patients polysensitized to pollen allergens, the priming effect, by which the sensitivity of the nasal mucosa to an allergen is increased by the previous exposure to another allergen, is a known phenomenon. This study was aimed at evaluating the degree of nasal inflammation, assessed by nasal cytology, in children with allergic rhinitis (AR) from ragweed pollen according to being monosensitized or polysensitized. Methods The study included 47 children. Of them, 24 suffered from AR caused by sensitization to grass pollen and ragweed pollen (group A) and 23 were sensitized only to ragweed pollen (group B). In all patients, the severity of AR was assessed according to the Allergic Rhinitis and Its Impact on Asthma guidelines, and comorbidities were also evaluated. Results In group A, 16.7% of children had a mild intermittent AR, 4.2% a moderate-to-severe intermittent, 33.3% a mild persistent, and 45.8% a moderate-to-severe persistent; in group B, 26.1% of children had a mild intermittent AR, 0% a moderate-to-severe intermittent, 52.2% a mild persistent, and 21.7% a moderate-to-severe persistent. No significant difference was detected in the number of the considered comorbidities between the two groups. The cell counts of neutrophils, eosinophils, lymphocytes/plasma cells, and mast cells were high but not significantly different in the two groups. Conclusion These findings show that the degree of nasal inflammation found in children with ragweed-induced AR is not influenced by additional allergy to grass pollen and confirm the previously reported absence of priming effect in ragweed allergy. PMID:27103838

  2. A Pollen-Specific RALF from Tomato That Regulates Pollen Tube Elongation12[W][OA

    PubMed Central

    Covey, Paul A.; Subbaiah, Chalivendra C.; Parsons, Ronald L.; Pearce, Gregory; Lay, Fung T.; Anderson, Marilyn A.; Ryan, Clarence A.; Bedinger, Patricia A.

    2010-01-01

    Rapid Alkalinization Factors (RALFs) are plant peptides that rapidly increase the pH of plant suspension cell culture medium and inhibit root growth. A pollen-specific tomato (Solanum lycopersicum) RALF (SlPRALF) has been identified. The SlPRALF gene encodes a preproprotein that appears to be processed and released from the pollen tube as an active peptide. A synthetic SlPRALF peptide based on the putative active peptide did not affect pollen hydration or viability but inhibited the elongation of normal pollen tubes in an in vitro growth system. Inhibitory effects of SlPRALF were detectable at concentrations as low as 10 nm, and complete inhibition was observed at 1 μm peptide. At least 10-fold higher levels of alkSlPRALF, which lacks disulfide bonds, were required to see similar effects. A greater effect of peptide was observed in low-pH-buffered medium. Inhibition of pollen tube elongation was reversible if peptide was removed within 15 min of exposure. Addition of 100 nm SlPRALF to actively growing pollen tubes inhibited further elongation until tubes were 40 to 60 μm in length, after which pollen tubes became resistant to the peptide. The onset of resistance correlated with the timing of the exit of the male germ unit from the pollen grain into the tube. Thus, exogenous SlPRALF acts as a negative regulator of pollen tube elongation within a specific developmental window. PMID:20388667

  3. Phosphoproteomics Profiling of Tobacco Mature Pollen and Pollen Activated in vitro.

    PubMed

    Fíla, Jan; Radau, Sonja; Matros, Andrea; Hartmann, Anja; Scholz, Uwe; Feciková, Jana; Mock, Hans-Peter; Čapková, Věra; Zahedi, René Peiman; Honys, David

    2016-04-01

    Tobacco mature pollen has extremely desiccated cytoplasm, and is metabolically quiescent. Upon re-hydration it becomes metabolically active and that results in later emergence of rapidly growing pollen tube. These changes in cytoplasm hydration and metabolic activity are accompanied by protein phosphorylation. In this study, we subjected mature pollen, 5-min-activated pollen, and 30-min-activated pollen to TCA/acetone protein extraction, trypsin digestion and phosphopeptide enrichment by titanium dioxide. The enriched fraction was subjected to nLC-MS/MS. We identified 471 phosphopeptides that carried 432 phosphorylation sites, position of which was exactly matched by mass spectrometry. These 471 phosphopeptides were assigned to 301 phosphoproteins, because some proteins carried more phosphorylation sites. Of the 13 functional groups, the majority of proteins were put into these categories: transcription, protein synthesis, protein destination and storage, and signal transduction. Many proteins were of unknown function, reflecting the fact that male gametophyte contains many specific proteins that have not been fully functionally annotated. The quantitative data highlighted the dynamics of protein phosphorylation during pollen activation; the identified phosphopeptides were divided into seven groups based on the regulatory trends. The major group comprised mature pollen-specific phosphopeptides that were dephosphorylated during pollen activation. Several phosphopeptides representing the same phosphoprotein had different regulation, which pinpointed the complexity of protein phosphorylation and its clear functional context. Collectively, we showed the first phosphoproteomics data on activated pollen where the position of phosphorylation sites was clearly demonstrated and regulatory kinetics was resolved. PMID:26792808

  4. Toolsets for Airborne Data

    Atmospheric Science Data Center

    2015-04-02

    article title:  Toolsets for Airborne Data     View larger image The ... limit of detection values. Prior to accessing the TAD Web Application ( https://tad.larc.nasa.gov ) for the first time, users must ...

  5. The airborne laser

    NASA Astrophysics Data System (ADS)

    Lamberson, Steven; Schall, Harold; Shattuck, Paul

    2007-05-01

    The Airborne Laser (ABL) is an airborne, megawatt-class laser system with a state-of-the-art atmospheric compensation system to destroy enemy ballistic missiles at long ranges. This system will provide both deterrence and defense against the use of such weapons during conflicts. This paper provides an overview of the ABL weapon system including: the notional operational concept, the development approach and schedule, the overall aircraft configuration, the technologies being incorporated in the ABL, and the current program status.

  6. Evolutionarily conserved phenylpropanoid pattern on angiosperm pollen.

    PubMed

    Fellenberg, Christin; Vogt, Thomas

    2015-04-01

    The male gametophyte of higher plants appears as a solid box containing the essentials to transmit genetic material to the next generation. These consist of haploid generative cells that are required for reproduction, and an invasive vegetative cell producing the pollen tube, both mechanically protected by a rigid polymer, the pollen wall, and surrounded by a hydrophobic pollen coat. This coat mediates the direct contact to the biotic and abiotic environments. It contains a mixture of compounds required not only for fertilization but also for protection against biotic and abiotic stressors. Among its metabolites, the structural characteristics of two types of phenylpropanoids, hydroxycinnamic acid amides and flavonol glycosides, are highly conserved in Angiosperm pollen. Structural and functional aspects of these compounds will be discussed. PMID:25739656

  7. Molecular biomarkers for grass pollen immunotherapy

    PubMed Central

    Popescu, Florin-Dan

    2014-01-01

    Grass pollen allergy represents a significant cause of allergic morbidity worldwide. Component-resolved diagnosis biomarkers are increasingly used in allergy practice in order to evaluate the sensitization to grass pollen allergens, allowing the clinician to confirm genuine sensitization to the corresponding allergen plant sources and supporting an accurate prescription of allergy immunotherapy (AIT), an important approach in many regions of the world with great plant biodiversity and/or where pollen seasons may overlap. The search for candidate predictive biomarkers for grass pollen immunotherapy (tolerogenic dendritic cells and regulatory T cells biomarkers, serum blocking antibodies biomarkers, especially functional ones, immune activation and immune tolerance soluble biomarkers and apoptosis biomarkers) opens new opportunities for the early detection of clinical responders for AIT, for the follow-up of these patients and for the development of new allergy vaccines. PMID:25237628

  8. Quarternary Pollen Analysis in Secondary School Ecology

    ERIC Educational Resources Information Center

    Slater, F. M.

    1972-01-01

    Describes techniques for studying historic changes in climate by analysis of pollen preserved in peat bogs. Illustrates the methodology and data analysis techniques by reference to results from English research. (AL)

  9. Juniper Pollen Hotspots in the Southwest

    NASA Technical Reports Server (NTRS)

    Bunderson, L. D.; VandeWater, P.; Luvall, J.; Levetin, E.

    2013-01-01

    Rationale: Juniperus pollen is a major allergen in Texas, Oklahoma, and New Mexico. While the bulk of pollen may be released in rural areas, large amounts of pollen can be transported to urban areas. Major juniper species in the region include: Juniperus ashei, J. virginiana, J. pinchotii, and J. monosperma. Pollen release is virtually continuous beginning in late September with J. pinchotii and ending in May with J. monosperma. Urban areas in the region were evaluated for the potential of overlapping seasons in order to inform sensitive individuals. Methods: Burkard volumetric pollen traps were established for two consecutive spring seasons at 6 sites in northern New Mexico and 6 sites for two consecutive winter and fall seasons in Texas and Oklahoma Standard methods were used in the preparation and analysis of slides. Results: The Dallas-Fort Worth Metroplex is home to over 6 million people. It is adjacent to populations of J. pinchotii, J. virginiana, and J. ashei. Peak concentration near Dallas for J. ashei in 2011 was 5891 pollen grains/m3 in January 7th. The peak date for J. pinchotii at an upwind sampling location in San Marcos, TX was November 1, 2010 and peak for J. virginiana at a nearby station in Tulsa, OK was November 1, 2010 and peak for J. virginiana at a nearby station in Tulsa, OK was February 20, 2011. Amarillo, TX is adjacent to J. pinchotii, J. ashei, and J. monosperma populations and may be subject to juniper pollen from September through May. Conclusions: Considering the overlapping distributions of juniper trees and the overlapping temporal release of pollen, sensitive patients may benefit from avoiding hotspots.

  10. Effect of artificial feeders on pollen loads of the hummingbirds of Cerro de la Muerte, Costa Rica.

    PubMed

    Avalos, Gerardo; Soto, Alejandra; Alfaro, Willy

    2012-03-01

    Although sugar-water feeders are commonly used by enthusiasts to attract hummingbirds, little is known about how they affect hummingbird behavior and flower use. We studied the highland hummingbird assemblage of Cerro de La Muerte, Costa Rica, both at a site with permanent feeders (La Georgina Restaurant) and further from it. We examined how feeder use and monopolization affected seasonal changes in pollen loads during four sampling periods, including dry and wet seasons, from 2003-2005. We expected that species monopolizing the feeders would carry little or no pollen whatsoever, and would have pollen loads characterized by low floral diversity, in contrast with species less dependent on feeders. We obtained pollen samples from 183 individuals of four hummingbird species captured around the feeders using mist nets, which were compared with a pollen reference collection of plants with a pollination syndrome by hummingbirds. The same methods were implemented at a site 3km away from the feeders. Feeder usage was quantified by counting the number of times hummingbirds drank from the feeders in periods of 4min separated by 1min. The effects of hummingbird species and season on pollen load categories were assessed using a nominal logistic regression. The alpha species at the site, the Fiery-throated Hummingbird (Panterpe insignis), dominated the feeders during the dry season. Meanwhile, in the wet season, feeder usage was more evenly distributed across species, with the exception of the Volcano Hummingbird, Selasphorus flammula, which occupies the last place in the dominance hierarchy. Pollen loads of hummingbirds captured near feeders were low in abundance (more than 50% of captured individuals had zero or low pollen loads), and low in species richness (96% of the hummingbirds with pollen from only one plant genus, Centropogon). Overall pollen loads increased during the dry season coinciding with peaks in flower availability, although the majority of captured

  11. Pollen as atmospheric cloud condensation nuclei

    NASA Astrophysics Data System (ADS)

    Steiner, Allison L.; Brooks, Sarah D.; Deng, Chunhua; Thornton, Daniel C. O.; Pendleton, Michael W.; Bryant, Vaughn

    2015-05-01

    Anemophilous (wind-dispersed) pollen grains are emitted in large quantities by vegetation in the midlatitudes for reproduction. Pollen grains are coarse particles (5-150 µm) that can rupture when wet to form submicron subpollen particles (SPP) that may have a climatic role. Laboratory CCN experiments of six fresh pollen samples show that SPP activate as CCN at a range of sizes, requiring supersaturations from 0.81 (± 0.07)% for 50 nm particles, 0.26 (± 0.03)% for 100 nm particles, and 0.12 (± 0.00)% for 200 nm particles. Compositional analyses indicate that SPP contain carbohydrates and proteins. The SPP contribution to global CCN is uncertain but could be important depending on pollen concentrations outside the surface layer and the number of SPP generated from a single pollen grain. The production of hygroscopic SPP from pollen represents a novel, biologically driven cloud formation pathway that may influence cloud optical properties and lifetimes, thereby influencing climate.

  12. Influence of Pollen Nutrition on Honey Bee Health: Do Pollen Quality and Diversity Matter?

    PubMed Central

    Di Pasquale, Garance; Salignon, Marion; Le Conte, Yves; Belzunces, Luc P.; Decourtye, Axel; Kretzschmar, André; Suchail, Séverine; Brunet, Jean-Luc; Alaux, Cédric

    2013-01-01

    Honey bee colonies are highly dependent upon the availability of floral resources from which they get the nutrients (notably pollen) necessary to their development and survival. However, foraging areas are currently affected by the intensification of agriculture and landscape alteration. Bees are therefore confronted to disparities in time and space of floral resource abundance, type and diversity, which might provide inadequate nutrition and endanger colonies. The beneficial influence of pollen availability on bee health is well-established but whether quality and diversity of pollen diets can modify bee health remains largely unknown. We therefore tested the influence of pollen diet quality (different monofloral pollens) and diversity (polyfloral pollen diet) on the physiology of young nurse bees, which have a distinct nutritional physiology (e.g. hypopharyngeal gland development and vitellogenin level), and on the tolerance to the microsporidian parasite Nosemaceranae by measuring bee survival and the activity of different enzymes potentially involved in bee health and defense response (glutathione-S-transferase (detoxification), phenoloxidase (immunity) and alkaline phosphatase (metabolism)). We found that both nurse bee physiology and the tolerance to the parasite were affected by pollen quality. Pollen diet diversity had no effect on the nurse bee physiology and the survival of healthy bees. However, when parasitized, bees fed with the polyfloral blend lived longer than bees fed with monofloral pollens, excepted for the protein-richest monofloral pollen. Furthermore, the survival was positively correlated to alkaline phosphatase activity in healthy bees and to phenoloxydase activities in infected bees. Our results support the idea that both the quality and diversity (in a specific context) of pollen can shape bee physiology and might help to better understand the influence of agriculture and land-use intensification on bee nutrition and health. PMID:23940803

  13. Mycotoxicological and palynological profiles of commercial brands of dried bee pollen

    PubMed Central

    Deveza, Michele Valadares; Keller, Kelly Moura; Lorenzon, Maria Cristina Affonso; Nunes, Lucila Maria Teixeira; Sales, Érika Oliveira; Barth, Ortrud Monika

    2015-01-01

    Abstract Pollen is used in the human diet as a food supplement because of its high nutritional value; however, this product is prone to fungal contamination that could potentially generate toxins that are harmful to human health. This study aimed to verify the floral diversity of commercial brands of bee pollen and their mycotoxicological safety for human consumption. A total of 27 bee pollen samples were analyzed; these samples represented commercial brands, either showing an inspection seal or not, marketed in the State of Rio de Janeiro. The analyzed parameters included floral diversity through palynological analysis, water activity, fungal counts, identification and toxigenic profiles. The palynological analysis identified nine plant families, of which the Asteraceae was predominant. Analysis of hygienic quality based on fungal load showed that 92% of samples were reproved according to the commercial, sanitary, and food safety quality indicators. Aspergillus, Cladosporium and Penicillium were the most common genera. Toxigenic evaluation showed that 25% of the A. flavus strains produced aflatoxins. The high rate of contamination of products bearing an inspection seal emphasizes the need to monitor the entire procedure of bee pollen production, as well as to revise the current legislation to ensure safe commercialization of this product. PMID:26691478

  14. On the development of probabilistic climate reconstructions based on fossil pollen records

    NASA Astrophysics Data System (ADS)

    Schoelzel, C.; Hense, A.

    2009-04-01

    Past climate reconstructions based on proxy data help to understand natural climate variability. It is essential that palaeoenvironmental transfer functions between climate and proxy variables take in to account the stochastical nature of the climate system. Especially pollen based reconstructions are a complex issue, since pollen counts do not show direct relation to climatological parameters, e.g. due to plant competition and/or land cultivation. The idea is to assess the background knowledge from classical, more empirically based methods in order to develop statistical transfer functions between vegetation and climate. Two popular concepts, the indicator taxa approach (or MCR) as well as the biomisation (BIOME/PFT), are translated into a statistical framework in order to reconstruct January and July temperatures as well as annual precipitation during the Holocene (10,000yrs BP). This involves the problem of multivariate random vectors with non-normally distributed marginals, which has been adressed by using copulas. The methods are applied to fossil pollen records of laminated sediments from the Eifel region in Germany. On the one hand, the reconstructions indicate that pollen based reconstructions show larger uncertainty ranges than expected by several classical approaches. On the other hand, the indicator taxa approach is robust to the lack of modern analogues, which is an advantage in highly anthropogenically influenced areas such as the Mediterranean or Central Europe.

  15. Mycotoxicological and palynological profiles of commercial brands of dried bee pollen.

    PubMed

    Deveza, Michele Valadares; Keller, Kelly Moura; Lorenzon, Maria Cristina Affonso; Nunes, Lucila Maria Teixeira; Sales, Érika Oliveira; Barth, Ortrud Monika

    2015-01-01

    Pollen is used in the human diet as a food supplement because of its high nutritional value; however, this product is prone to fungal contamination that could potentially generate toxins that are harmful to human health. This study aimed to verify the floral diversity of commercial brands of bee pollen and their mycotoxicological safety for human consumption. A total of 27 bee pollen samples were analyzed; these samples represented commercial brands, either showing an inspection seal or not, marketed in the State of Rio de Janeiro. The analyzed parameters included floral diversity through palynological analysis, water activity, fungal counts, identification and toxigenic profiles. The palynological analysis identified nine plant families, of which the Asteraceae was predominant. Analysis of hygienic quality based on fungal load showed that 92% of samples were reproved according to the commercial, sanitary, and food safety quality indicators. Aspergillus, Cladosporium and Penicillium were the most common genera. Toxigenic evaluation showed that 25% of the A. flavus strains produced aflatoxins. The high rate of contamination of products bearing an inspection seal emphasizes the need to monitor the entire procedure of bee pollen production, as well as to revise the current legislation to ensure safe commercialization of this product. PMID:26691478

  16. [Factors affecting the estimation of pollen limitation in Sagittaria trifolia].

    PubMed

    Qin, Dao-feng; Li, Ting; Dai, Can

    2015-12-01

    This study explored whether the degree of pollen limitation was affected by the experimental level (a single flower or inflorescence) and pollen quality (self-pollen or outcross-pollen) of supplemental pollination in Sagittaria trifolia. The results showed that the experimental level caused varying degree of pollen limitation. Compared with the inflorescence level, pollination at the single flower level led to a redistribution of resources among flowers, therefore affecting seed numbers. Pollen quality also played a vital role in the estimation of pollen limitation. Compared with self-pollen, supplemental pollination with outcross-pollen resulted in significantly more seeds and a higher germination rate. This proved that in the research system the reproduction was limited by pollen quality rather than quantity. Our study revealed that both experimental level and pollen quality had effects on the estimation of pollen limitation. It was suggested that in future studies we should evaluate pollen limitation at the inflorescence or whole plant level, and also consider comparing self- and outcross-pollen when applicable. PMID:27112030

  17. Does an 'oversupply' of ovules cause pollen limitation?

    PubMed

    Rosenheim, Jay A; Schreiber, Sebastian J; Williams, Neal M

    2016-04-01

    Lifetime seed production can be constrained by shortfalls of pollen receipt ('pollen limitation'). The ovule oversupply hypothesis states that, in response to unpredictable pollen availability, plants evolve to produce more ovules than they expect to be fertilized, and that this results in pollen limitation of seed production. Here, we present a cartoon model and a model of optimal plant reproductive allocations under stochastic pollen receipt to evaluate the hypothesis that an oversupply of ovules leads to increased pollen limitation. We show that an oversupply of ovules has two opposing influences on pollen limitation of whole-plant seed production. First, ovule oversupply increases the likelihood that pollen receipt limits the number of ovules that can be fertilized ('prezygotic pollen limitation'). Second, ovule oversupply increases the proportion of pollen grains received that are used to fertilize ovules ('pollen use efficiency'). As a result of these opposing influences, ovule oversupply has only a modest effect on the degree to which lifetime seed production is constrained by pollen receipt, producing a small decrease in the incidence of pollen limitation. Ovule oversupply is not the cause of the pollen limitation problem, but rather is part of the evolutionary solution to that problem. PMID:26574903

  18. A 12,000-Yr Pollen Record off Cape Hatteras: Pollen Sources and Mechanisms of Pollen Dispersion

    NASA Technical Reports Server (NTRS)

    Naughton, F.; Keigwin, L.; Peteet, D.; Costas, S.; Desprat, S.; Oliveira, D.; de Vernal, A.; Voelker, A.; Abrantes, F.

    2015-01-01

    Integrating both marine and terrestrial signals from the same sediment core is one of the primary challenges for understanding the role of ocean-atmosphere coupling throughout past climate changes. It is therefore vital to understand how the pollen signal of a given marine record reflects the vegetation changes of the neighboring continent. The comparison between the pollen record of marine core JPC32 (KNR178JPC32) and available terrestrial pollen sequences from eastern North America over the last 12,170 years indicates that the pollen signature off Cape Hatteras gives an integrated image of the regional vegetation encompassing the Pee Dee river, Chesapeake and Delaware hydrographic basins and is reliable in reconstructing the past climate of the adjacent continent. Extremely high quantities of pollen grains included in the marine sediments off Cape Hatteras were transferred from the continent to the sea, at intervals 10,100-8800 cal yr BP, 8300-7500 cal yr BP, 5800- 4300 cal yr BP and 2100-730 cal yr BP, during storm events favored by episodes of rapid sea-level rise in the eastern coast of US. In contrast, pollen grains export was reduced during 12,170-10,150 cal yr BP and 4200- 2200 cal yr BP, during episodes of intense continental dryness and slow sea level rise episodes or lowstands in the eastern coast of US. The near absence of reworked pollen grains in core JPC32 contrasts with the high quantity of reworked material in nearby but deeper located marine sites, suggesting that the JPC32 recordwas not affected by the DeepWestern Boundary Current (DWBC) since the end of the Younger Dryas and should be considered a key site for studying past climate changes in the western North Atlantic.

  19. Food for Pollinators: Quantifying the Nectar and Pollen Resources of Urban Flower Meadows.

    PubMed

    Hicks, Damien M; Ouvrard, Pierre; Baldock, Katherine C R; Baude, Mathilde; Goddard, Mark A; Kunin, William E; Mitschunas, Nadine; Memmott, Jane; Morse, Helen; Nikolitsi, Maria; Osgathorpe, Lynne M; Potts, Simon G; Robertson, Kirsty M; Scott, Anna V; Sinclair, Frazer; Westbury, Duncan B; Stone, Graham N

    2016-01-01

    Planted meadows are increasingly used to improve the biodiversity and aesthetic amenity value of urban areas. Although many 'pollinator-friendly' seed mixes are available, the floral resources these provide to flower-visiting insects, and how these change through time, are largely unknown. Such data are necessary to compare the resources provided by alternative meadow seed mixes to each other and to other flowering habitats. We used quantitative surveys of over 2 million flowers to estimate the nectar and pollen resources offered by two exemplar commercial seed mixes (one annual, one perennial) and associated weeds grown as 300m2 meadows across four UK cities, sampled at six time points between May and September 2013. Nectar sugar and pollen rewards per flower varied widely across 65 species surveyed, with native British weed species (including dandelion, Taraxacum agg.) contributing the top five nectar producers and two of the top ten pollen producers. Seed mix species yielding the highest rewards per flower included Leontodon hispidus, Centaurea cyanus and C. nigra for nectar, and Papaver rhoeas, Eschscholzia californica and Malva moschata for pollen. Perennial meadows produced up to 20x more nectar and up to 6x more pollen than annual meadows, which in turn produced far more than amenity grassland controls. Perennial meadows produced resources earlier in the year than annual meadows, but both seed mixes delivered very low resource levels early in the year and these were provided almost entirely by native weeds. Pollen volume per flower is well predicted statistically by floral morphology, and nectar sugar mass and pollen volume per unit area are correlated with flower counts, raising the possibility that resource levels can be estimated for species or habitats where they cannot be measured directly. Our approach does not incorporate resource quality information (for example, pollen protein or essential amino acid content), but can easily do so when suitable data

  20. Food for Pollinators: Quantifying the Nectar and Pollen Resources of Urban Flower Meadows

    PubMed Central

    Hicks, Damien M.; Ouvrard, Pierre; Baldock, Katherine C. R.; Baude, Mathilde; Goddard, Mark A.; Kunin, William E.; Mitschunas, Nadine; Memmott, Jane; Morse, Helen; Nikolitsi, Maria; Osgathorpe, Lynne M.; Potts, Simon G.; Robertson, Kirsty M.; Scott, Anna V.; Sinclair, Frazer; Westbury, Duncan B.; Stone, Graham N.

    2016-01-01

    Planted meadows are increasingly used to improve the biodiversity and aesthetic amenity value of urban areas. Although many ‘pollinator-friendly’ seed mixes are available, the floral resources these provide to flower-visiting insects, and how these change through time, are largely unknown. Such data are necessary to compare the resources provided by alternative meadow seed mixes to each other and to other flowering habitats. We used quantitative surveys of over 2 million flowers to estimate the nectar and pollen resources offered by two exemplar commercial seed mixes (one annual, one perennial) and associated weeds grown as 300m2 meadows across four UK cities, sampled at six time points between May and September 2013. Nectar sugar and pollen rewards per flower varied widely across 65 species surveyed, with native British weed species (including dandelion, Taraxacum agg.) contributing the top five nectar producers and two of the top ten pollen producers. Seed mix species yielding the highest rewards per flower included Leontodon hispidus, Centaurea cyanus and C. nigra for nectar, and Papaver rhoeas, Eschscholzia californica and Malva moschata for pollen. Perennial meadows produced up to 20x more nectar and up to 6x more pollen than annual meadows, which in turn produced far more than amenity grassland controls. Perennial meadows produced resources earlier in the year than annual meadows, but both seed mixes delivered very low resource levels early in the year and these were provided almost entirely by native weeds. Pollen volume per flower is well predicted statistically by floral morphology, and nectar sugar mass and pollen volume per unit area are correlated with flower counts, raising the possibility that resource levels can be estimated for species or habitats where they cannot be measured directly. Our approach does not incorporate resource quality information (for example, pollen protein or essential amino acid content), but can easily do so when suitable

  1. Detection of changes in the nuclear phase and evaluation of male germ units by flow cytometry during in vitro pollen tube growth in Alstroemeria aurea.

    PubMed

    Hirano, Tomonari; Hoshino, Yoichiro

    2009-03-01

    This study aimed to analyze male gamete behavior from mature pollen to pollen tube growth in the bicellular pollen species Alstroemeria aurea. For mature pollen, pollen protoplasts were examined using flow cytometry. The protoplasts showed two peaks of DNA content at 1C and 1.90C. Flow cytometry at different developmental stages of pollen tubes cultured in vitro revealed changes in the nuclear phase at 9 and 18 h after culture. Sperm cell formation occurred at 6-9 h after culture, indicating that the first change was due to the division of the generative cells into sperm cells. After sperm cell formation, the number of vegetative nucleus associations with sperm cells showed a tendency to increase. This association was suggested as the male germ unit (MGU). When sperm cells, vegetative nuclei, and partial MGUs were collected separately from pollen tubes cultured for 18 h and analyzed using a flow cytometer, the sperm cells and vegetative nuclei contained 1C DNA, while the DNA content of partial MGUs was counted as 2C. Therefore, the second change in the nuclear phase, which results in an increase in 2C nuclei, is possibly related to the formation of MGUs. PMID:19151916

  2. Factors that determine the severity of Betula spp. pollen seasons in Poland (Poznań and Krakow) and the United Kingdom (Worcester and London)

    NASA Astrophysics Data System (ADS)

    Stach, A.; Emberlin, J.; Smith, M.; Adams-Groom, B.; Myszkowska, D.

    2008-03-01

    The aim of this paper is to analyse variations in the severity of Betula pollen seasons, particularly in relation to meteorological parameters at four sites, Poznań and Krakow in Poland, and Worcester and London in the United Kingdom. Results show that there is a significant relationship between Betula pollen season severity and weather conditions both in the year before pollination and in the same year that pollen is released from the plant. Furthermore, it is likely that the magnitude of birch pollen seasons in Poznań, Worcester and London is linked in some way to different phases of the North Atlantic Oscillation (NAO). Significant positive relationships exist between birch pollen counts at Poznań and temperatures, rainfall and averages of the NAO in the year before pollination. An opposite relationship is evident at the two sites studied in the United Kingdom. There were significant positive correlations between the severity of birch pollen seasons recorded at Worcester and temperatures and averages of the NAO during the winter and spring in the year of pollination, and negative correlations at both Worcester and London with similar variables from the previous year. In addition, Betula pollen seasons in Krakow do not appear to be influenced by the NAO, which is probably the result of Krakow having a more continental climate.

  3. Design of a downscaling method to estimate continuous data from discrete pollen monitoring in Tunisia.

    PubMed

    Orlandi, Fabio; Oteros, Jose; Aguilera, Fátima; Ben Dhiab, Ali; Msallem, Monji; Fornaciari, Marco

    2014-07-01

    The study of microorganisms and biological particulate matter that transport passively through air is very important for an understanding of the real quality of air. Such monitoring is essential in several specific areas, such as public health, allergy studies, agronomy, indoor and outdoor conservation, and climate-change impact studies. Choosing the suitable monitoring method is an important step in aerobiological studies, so as to obtain reliable airborne data. In this study, we compare olive pollen data from two of the main air traps used in aerobiology, the Hirst and Cour air samplers, at three Tunisian sampling points, for 2009 to 2011. Moreover, a downscaling method to perform daily Cour air sampler data estimates is designed. While Hirst air samplers can offer daily, and even bi-hourly data, Cour air samplers provide data for longer discrete sampling periods, which limits their usefulness for daily monitoring. Higher quantities of olive pollen capture were generally detected for the Hirst air sampler, and a downscaling method that is developed in this study is used to model these differences. The effectiveness of this downscaling method is demonstrated, which allows the potential use of Cour air sampler data series. These results improve the information that new Cour data and, importantly, historical Cour databases can provide for the understanding of phenological dates, airborne pollination curves, and allergenicity levels of air. PMID:24824947

  4. Morphology and structure of the pollen cone and pollen grain of the Araucaria species from Argentina.

    PubMed

    Del Fueyo, Georgina M; Caccavari, Marta A; Dome, Elizabeth A

    2008-04-01

    The pollen cone and the pollen grain of the two Argentinean species of Araucaria are described with LM, SEM and TEM. Primordia of pollen cones are formed in April and May and reach maturity by mid-October in A. angustifolia (Bert.) O. Kuntze and by mid-November in A. araucana. (Mol.) K. Koch. Characters of the mature pollen cones and microsporophylls between both taxa are clearly differentiated. Pollen grains are spheroidal-subspheroidal, inaperturate, and asaccate with granulate exine and a subequatorial annular area that corresponds to the sexine thickness. Sculpturing consists of irregularly dispersed granules that are sometimes fused to each other (A. angustifolia) or forming microrugulae (A. araucana). Microgranules and microspinules are also present. The pollen wall ultrastructure is formed by a granular ectexine and lamellated endexine. Granular elements in A. angustifolia are more loosely disposed, form more interstices, and are gradually smaller towards the endexine than in A. araucana. To asses the probable relationships within the family, we compared the pollen grains of the two Araucaria species with those of other extant genera (Agathis, Wollemia) and also with fossil pollen (Araucariacites, Balmeiopsis, Cyclusphaera, Dilwynites) attributed to Araucariaceae. PMID:18669323

  5. Ultraviolet radiation environment of pollen and its effect on pollen germination. Final report

    SciTech Connect

    Not Available

    1981-12-01

    The damage to pollen caused by natural ultraviolet radiation was investigated. Experimental and literature research into the UV radiation environment is reported. Viability and germination of wind and insect pollinated species were determined. Physiological, developmental, and protective factors influencing UV sensitivity of binucleate, advanced binucleate, and trinucleate pollen grains are compared.

  6. The ultraviolet radiation environment of pollen and its effect on pollen germination

    NASA Technical Reports Server (NTRS)

    1981-01-01

    The damage to pollen caused by natural ultraviolet radiation was investigated. Experimental and literature research into the UV radiation environment is reported. Viability and germination of wind and insect pollinated species were determined. Physiological, developmental, and protective factors influencing UV sensitivity of binucleate, advanced binucleate, and trinucleate pollen grains are compared.

  7. Analysis of Allergenic Pollen by FTIR Microspectroscopy.

    PubMed

    Zimmerman, B; Tafintseva, V; Bağcıoğlu, M; Høegh Berdahl, M; Kohler, A

    2016-01-01

    Fourier transform infrared (FTIR) spectroscopy is a powerful tool for the identification and characterization of pollen and spores. However, interpretation and multivariate analysis of infrared microscopy spectra of single pollen grains are hampered by Mie-type scattering. In this paper, we introduce a novel sampling setup for infrared microspectroscopy of pollens preventing strong Mie-type scattering. Pollen samples were embedded in a soft paraffin layer between two sheets of polyethylene foils without any further sample pretreatment. Single-grain infrared spectra of 13 different pollen samples, belonging to 11 species, were obtained and analyzed by the new approach and classified by sparse partial least-squares regression (PLSR). For the classification, chemical and physical information were separated by extended multiplicative signal correction and used together to build a classification model. A training set of 260 spectra and an independent test set of 130 spectra were used. Robust sparse classification models allowing the biochemical interpretation of the classification were obtained by the sparse PLSR, because only a subset of variables was retained for the analysis. With accuracy values of 95% and 98%, for the independent test set and full cross-validation respectively, the method is outperforming the previously published studies on development of an automated pollen analysis. Since the method is compatible with standard air-samplers, it can be employed with minimal modification in regular aerobiology studies. When compared with optical microscopy, which is the benchmark method in pollen analysis, the infrared microspectroscopy method offers better taxonomic resolution, as well as faster, more economical, and bias-free measurement. PMID:26599685

  8. Wind-pollination and the roles of pollen allergenic proteins.

    PubMed

    Songnuan, Wisuwat

    2013-12-01

    Over the past few decades, there has been an explosion of understanding of the molecular nature of major allergens contained within pollens from the most important allergenic plant species. Most major allergens belong to only a few protein families. Protein characteristics, cross-reactivity, structures, and IgE binding epitopes have been determined for several allergens. These efforts have led to significant improvements in specific immunotherapy, yet there has been little discussion about the physiological functions of these proteins. Even with large amounts of available information about allergenic proteins from pollens, the incidence of pollen allergy continuously increases worldwide. The reason for this increase is unclear and is most likely due to a combination of factors. One important culprit might be a change in the pollen itself. Knowledge about pollen biology and how pollen is changing as a result of more extreme environmental conditions might improve our understanding of the disease. This review focuses on the characteristics of plants producing allergenic pollens that are relevant to pollen allergy, including the phylogenetic relationships, pollen dispersal distances, amounts of pollen produced, amounts of protein in each type of pollen, and how allergenic proteins are released from pollens. In addition, the physiological roles of major allergenic protein families will be discussed to help us understand why some of these proteins become allergens and why GMO plants with hypoallergenic pollens may not be successful. PMID:24383968

  9. Bees associate colour cues with differences in pollen rewards.

    PubMed

    Nicholls, Elizabeth; de Ibarra, Natalie Hempel

    2014-08-01

    In contrast to the wealth of knowledge concerning sucrose-rewarded learning, the question of whether bees learn when they collect pollen from flowers has been little addressed. The nutritional value of pollen varies considerably between species, and it may be that bees learn the features of flowers that produce pollen best suited to the dietary requirements of their larvae. It is still unknown, however, whether a non-ingestive reward pathway for pollen learning exists, and how foraging bees sense differences between pollen types. Here we adopt a novel experimental approach testing the learning ability of bees with pollen rewards. Bumblebees were reared under controlled laboratory conditions. To establish which pollen rewards are distinguishable, individual bees were given the choice of collecting two types of pollen, diluted to varying degrees with indigestible α-cellulose. Bees preferentially collected a particular pollen type, but this was not always the most concentrated sample. Preferences were influenced by the degree of similarity between samples and also by the period of exposure, with bees more readily collecting samples of lower pollen concentration after five trials. When trained differentially, bees were able to associate an initially less-preferred contextual colour with the more concentrated sample, whilst their pollen preferences did not change. Successful learning of contextual cues seems to maintain pollen foraging preferences over repeated exposures, suggesting that fast learning of floral cues may preclude continuous sampling and evaluation of alternative reward sources, leading to constancy in pollen foraging. PMID:24855678

  10. Recent pollen spectra and zonal vegetation in the western USSR

    NASA Astrophysics Data System (ADS)

    Peterson, G. M.

    The relationship of modern pollen spectra to present-day vegetation is critical to the reconstruction of vegetation and climate from fossil pollen spectra. This study uses isopoll maps to illustrate the pollen-vegetation relationships in the Soviet Union west of 100°E and presents descriptive statistics for 544 modern samples of arboreal pollen and for 370 samples of herb pollen obtained from the Soviet palynological literature. Data are assembled from this large geographic region and presented in a standardized form on a scale which can be used to relate quantitative pollen data to zonal vegetation and climatic variables and to make comparisons with other regions. In order to show the relationship between pollen types and major ecotones in forested and non-forested areas, the pollen data are presented as percentages of a sum including both arboreal and non-arboreal pollen. Major pollen types which attain values of 10% or more in at least one vegetation zone include Betula (birch), Cyperaceae (sedges), Picea (spruce), Pinus (total pine), Pinus sibirica, Ericaceae (heath family), Gramineae (grasses), Artemisia (sage), and Chenopodiaceae (i.e., saltbush, Russian thistle, pigweed family). Samples from the tundra and forest-tundra have high values of Ericaceae (heath family), birch, alder, and sedge pollen. In the boreal forest, pine, spruce, and birch pollen predominate. In the mixed and deciduous forests, Tilia (linden), Quercus (oak), Ulmus (elm), and Corylus (hazel) pollen attain maximum values. In the forest-steppe and steppe zones, arboreal pollen decreases in importance and is replaced by non-arboreal pollen types. Pollen of Artemisia and Chenopodiaceae predominates in the semi-desert zones. In spite of variation in the pollen spectra arising from the use of different sediment types (soil, peat, and river sediments), and human disturbance of vegetation, the pollen spectra are clearly related to zonal vegetation. Pollen spectra from the western USSR show

  11. Allergenicity of the pollen of Pistacia.

    PubMed

    Keynan, N; Tamir, R; Waisel, Y; Reshef, A; Spitz, E; Shomer-Ilan, A; Geller-Bernstein, C

    1997-03-01

    Differences in IgE binding and skin responses to pollen extracts of four species of Pistacia, and some immunochemical characteristics of this pollen were investigated. The incidence of positive SPT among atopic patients varied between 31.5% to the pollen extracts of P. vera and 24.6% to P. palaestina. The antigens are located on the exine of the grains as well as in their cytoplasm. Some of the antigens are common to all four species, whereas others seem to be specific. Cross-reactivity was found among the four species of Pistacia and between them and Schinus terebintifolious. Five conspicuous IgE-binding bands were observed in the immunoblots of the four examined species, the bands of 49, 57, 64, 68, and 79 kDa. The 36-37-kDa band of P. lentiscus and the 60- and 84-kDa bands of P. atlantica and P. vera were also noticeable. As the flowering seasons of Pistacia and Schinus do not overlap, the patients are exposed to such pollen for more than 4 months a year. Apparently, Pistacia pollen is a major source of allergy. PMID:9140524

  12. Effect of water absorption on pollen adhesion.

    PubMed

    Lin, Haisheng; Lizarraga, Leonardo; Bottomley, Lawrence A; Carson Meredith, J

    2015-03-15

    Pollens possess a thin liquid coating, pollenkitt, which plays a major role in adhesion by forming capillary menisci at interfaces. Unfortunately, the influence of humidity on pollenkitt properties and capillary adhesion is unknown. Because humidity varies widely in the environment, the answers have important implications for better understanding plant reproduction, allergy and asthma, and pollen as atmospheric condensation nuclei. Here, pollenkitt-mediated adhesion of sunflower pollen to hydrophilic and hydrophobic surfaces was measured as a function of humidity. The results quantify for the first time the significant water absorption of pollenkitt and the resulting complex dependence of adhesion on humidity. On hydrophilic Si, adhesion increased with increasing RH for pollens with or without pollenkitt, up to 200nN at 70% RH. In contrast, on hydrophobic PS, adhesion of pollenkitt-free pollen is independent of RH. Surprisingly, when pollenkitt was present adhesion forces on hydrophobic PS first increased with RH up to a maximum value at 35% RH (∼160nN), and then decreased with further increases in RH. Independent measurement of pollenkitt properties is used with models of capillary adhesion to show that humidity-dependent changes in pollenkitt wetting and viscosity are responsible for this complex adhesion behavior. PMID:25524008

  13. The Airborne Laser

    NASA Astrophysics Data System (ADS)

    Lamberson, Steven E.

    2002-09-01

    The US Air Force Airborne Laser (ABL) is an airborne, megawatt-class laser system with a state-of-the-art atmospheric compensation system to destroy enemy ballistic missiles at long ranges. This system will provide both deterrence and defense against the use of such weapons during conflicts. This paper provides an overview of the ABL weapon system including: the notional operational concept, the development approach and schedule, the overall aircraft configuration, the technologies being incorporated in the ABL, and the risk reduction approach being utilized to ensure program success.

  14. Airborne oceanographic lidar system

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Specifications and preliminary design of an Airborne Oceanographic Lidar (AOL) system, which is to be constructed for installation and used on a NASA Wallops Flight Center (WFC) C-54 research aircraft, are reported. The AOL system is to provide an airborne facility for use by various government agencies to demonstrate the utility and practicality of hardware of this type in the wide area collection of oceanographic data on an operational basis. System measurement and performance requirements are presented, followed by a description of the conceptual system approach and the considerations attendant to its development. System performance calculations are addressed, and the system specifications and preliminary design are presented and discussed.

  15. Counting Sheep in Basque

    ERIC Educational Resources Information Center

    Araujo, Frank P.

    1975-01-01

    Demonstrates the interplay of a cognitive system, the Basque numerative system, and a behavioral one, counting sheep. The significant features of the Basque numerative system are analyzed; then it is shown how use of these features facilitates the counting of sheep on open ranges by Basque sheep farmers in California. (Author/RM)

  16. The Makah Counting Workbook.

    ERIC Educational Resources Information Center

    Flinn, Arlington A., Jr.

    The first edition of the counting workbook centers around the numbers from 1 to 100 and focuses on number and set concepts. The workbook introduces the Makah spelling of each number and reinforces the spelling with exercises such as matching words to numbers, writing the words, counting symbols, and circling the correct number. Spaced throughout…

  17. Complexities of Counting.

    ERIC Educational Resources Information Center

    Stake, Bernadine Evans

    This document focuses on one child's skip counting methods. The pupil, a second grade student at Steuben School, in Kankakee, Illinois, was interviewed as she made several attempts at counting twenty-five poker chips on a circular piece of paper. The interview was part of a larger study of "Children's Conceptions of Number and Numeral," funded by…

  18. NASA Airborne Lidar July 1991

    Atmospheric Science Data Center

    2016-05-26

    NASA Airborne Lidar July 1991 Data from the 1991 NASA Langley Airborne Lidar flights following the eruption of Pinatubo in July ... and Osborn [1992a, 1992b]. Project Title:  NASA Airborne Lidar Discipline:  Field Campaigns ...

  19. NASA Airborne Lidar May 1992

    Atmospheric Science Data Center

    2016-05-26

    NASA Airborne Lidar May 1992 An airborne Nd:YAG (532 nm) lidar was operated by the NASA Langley Research Center about a year following the June 1991 eruption of ... Osborn [1992a, 1992b].  Project Title:  NASA Airborne Lidar Discipline:  Field Campaigns ...

  20. Spring Allergies? Don't Assume It's Only Pollen

    MedlinePlus

    ... html Spring Allergies? Don't Assume It's Only Pollen Identifying your triggers is the first step toward ... reducing your symptoms, experts say. You may believe pollen is the culprit. But, other substances such as ...

  1. A Pollen Primer | NIH MedlinePlus the Magazine

    MedlinePlus

    ... please turn Javascript on. Feature: Managing Allergies A Pollen Primer Past Issues / Summer 2011 Table of Contents ... National Institute of Environmental Health Sciences (NIEHS) . Plant Pollen Ragweed and other weeds, such as curly dock, ...

  2. Quantification of airway deposition of intact and fragmented pollens.

    PubMed

    Horváth, Alpár; Balásházy, Imre; Farkas, Arpád; Sárkány, Zoltán; Hofmann, Werner; Czitrovszky, Aladár; Dobos, Erik

    2011-12-01

    Although pollen is one of the most widespread agents that can cause allergy, its airway transport and deposition is far from being fully explored. The objective of this study was to characterize the airway deposition of pollens and to contribute to the debate related to the increasing number of asthma attacks registered after thunderstorms. For the quantification of the deposition of inhaled pollens in the airways computer simulations were performed. Our results demonstrated that smaller and fragmented pollens may penetrate into the thoracic airways and deposit there, supporting the theory that fragmented pollen particles are responsible for the increasing incidence of asthma attacks following thunderstorms. Pollen deposition results also suggest that children are the most exposed to the allergic effects of pollens. Finally, pollens between 0.5 and 20 μm deposit more efficiently in the lung of asthmatics than in the healthy lung, especially in the bronchial region. PMID:21563012

  3. Climatic variations across the Mediterranean Basin reconstructed from pollen and vegetation model

    NASA Astrophysics Data System (ADS)

    Guiot, J.; Leydet, M.; Rotereau, A.

    2012-12-01

    The Mediterranean Basin is a complex region with a complex topography at the interface of two climatic systems. Water availability is the main limiting factor of vegetation at low elevation and both temperature and precipitation are equally important at higher elevation. We propose here a gridded reconstruction of the Holocene climate at the scale of the Mediterranean region from pollen by using a vegetation model in inverse mode (Guiot et al, 2009) and so relating vegetation changes to climatic changes in a more mechanistic way than standard statistical approaches. The proxy data used are the pollen series stored in the European Pollen Database (EPD) for the Mediterranean Basin. The period covered is the last 10 ky at a multi-decadal time-step and the climatic variables are winter, summer temperature and precipitation, as well as soil water. BIOME4 model (Kaplan et al, 2003) uses as inputs monthly temperature, precipitation variables and provides outputs comparable to pollen data (assuming that there is a relationship between plant productivity and pollen counts). The idea behind paleoclimatological reconstructions is then to obtain inputs, given outputs. This procedure, called model inversion, is achieved with appropriate algorithms in the frame of the Bayesian statistical theory. As CO2 atmospheric concentration is also an input of the model, it is possible to take into account the true variations of the concentration across Holocene to reconstruct the climate. We will present gridded maps of climatic change for typical periods where Mediterranean Basin has known important water stresses. Guiot, J. Et al, 2009. Climate of the Past, 5, 571-583. Kaplan, J., et al, 2003. JGR-Atmos., 108, 8171, doi:10.1029/2002JD002559

  4. [Hypersensitivity to pollen of Olea europea in patients with pollen allergy in Zadar County, Croatia].

    PubMed

    Skitarelić, Natasa; Mazzi, Antun; Skitarelić, Neven; Misulić, Josko; Vuletić, Ana

    2010-06-01

    Olive pollen is one of the most common respiratory allergens in the Mediterranean countries. The aim of this study was to establish the frequency of hypersensitivity to the pollen of Olea europea in pollen allergic patients in the County of Zadar. The study included 671 patients with pollen allergy; 61 % were male and 39 % female. 53.5 % were children aged from 4 to 14 years and 46.5 % adolescents and adults from 15 to 59 years. We took their case history, clinically examined them, and tested using the skin prick test and enzymo-immunologic UniCAP test for specific IgE antibodies. For statistical analysis we used the chi-square test. Hypersensitivity to Olea europea pollen was confirmed in 8.8 % patients with pollen allergy. Among them, the most prevalent symptom was rhinitis (58 %). Most hypersensitive patients were urban residents. Only 3 % patients lived on an island. Judging by available data, our findings show the lowest hypersensitivity to olive pollen in the Mediterranean. A comparison with our two earlier studies did not show any fluctuation in this kind of hypersensitivity. PMID:20587396

  5. A new 'bio-comfort' perspective for Melbourne based on heat stress, air pollution and pollen.

    PubMed

    Jacobs, Stephanie J; Pezza, Alexandre B; Barras, Vaughan; Bye, John

    2014-03-01

    Humans are at risk from exposure to extremes in their environment, yet there is no consistent way to fully quantify and understand the risk when considering more than just meteorological variables. An outdoor 'bio-comfort' threshold is defined for Melbourne, Australia using a combination of heat stress, air particulate concentration and grass pollen count, where comfortable conditions imply an ideal range of temperature, humidity and wind speed, acceptable levels of air particulates and a low pollen count. This is a new approach to defining the comfort of human populations. While other works have looked into the separate impacts of different variables, this is the first time that a unified bio-comfort threshold is suggested. Composite maps of surface pressure are used to illustrate the genesis and evolution of the atmospheric structures conducive to an uncomfortable day. When there is an uncomfortable day due to heat stress conditions in Melbourne, there is a high pressure anomaly to the east bringing warm air from the northern interior of Australia. This anomaly is part of a slow moving blocking high originating over the Indian Ocean. Uncomfortable days due to high particulate levels have an approaching cold front. However, for air particulate cases during the cold season there are stable atmospheric conditions enhanced by a blocking high emanating from Australia and linking with the Antarctic continent. Finally, when grass pollen levels are high, there are northerly winds carrying the pollen from rural grass lands to Melbourne, due to a stationary trough of low pressure inland. Analysis into days with multiple types of stress revealed that the atmospheric signals associated with each type of discomfort are present regardless of whether the day is uncomfortable due to one or multiple variables. Therefore, these bio-comfort results are significant because they offer a degree of predictability for future uncomfortable days in Melbourne. PMID:23404183

  6. A new `bio-comfort' perspective for Melbourne based on heat stress, air pollution and pollen

    NASA Astrophysics Data System (ADS)

    Jacobs, Stephanie J.; Pezza, Alexandre B.; Barras, Vaughan; Bye, John

    2014-03-01

    Humans are at risk from exposure to extremes in their environment, yet there is no consistent way to fully quantify and understand the risk when considering more than just meteorological variables. An outdoor `bio-comfort' threshold is defined for Melbourne, Australia using a combination of heat stress, air particulate concentration and grass pollen count, where comfortable conditions imply an ideal range of temperature, humidity and wind speed, acceptable levels of air particulates and a low pollen count. This is a new approach to defining the comfort of human populations. While other works have looked into the separate impacts of different variables, this is the first time that a unified bio-comfort threshold is suggested. Composite maps of surface pressure are used to illustrate the genesis and evolution of the atmospheric structures conducive to an uncomfortable day. When there is an uncomfortable day due to heat stress conditions in Melbourne, there is a high pressure anomaly to the east bringing warm air from the northern interior of Australia. This anomaly is part of a slow moving blocking high originating over the Indian Ocean. Uncomfortable days due to high particulate levels have an approaching cold front. However, for air particulate cases during the cold season there are stable atmospheric conditions enhanced by a blocking high emanating from Australia and linking with the Antarctic continent. Finally, when grass pollen levels are high, there are northerly winds carrying the pollen from rural grass lands to Melbourne, due to a stationary trough of low pressure inland. Analysis into days with multiple types of stress revealed that the atmospheric signals associated with each type of discomfort are present regardless of whether the day is uncomfortable due to one or multiple variables. Therefore, these bio-comfort results are significant because they offer a degree of predictability for future uncomfortable days in Melbourne.

  7. Modern pollen deposition in Long Island Sound

    USGS Publications Warehouse

    Beuning, Kristina R.M.; Fransen, Lindsey; Nakityo, Berna; Mecray, Ellen L.; Bucholtz ten Brink, Marilyn R.

    2000-01-01

    Palynological analyses of 20 surface sediment samples collected from Long Island Sound show a pollen assemblage dominated by Carya, Betula, Pinus, Quercus, Tsuga, and Ambrosia, as is consistent with the regional vegetation. No trends in relative abundance of these pollen types occur either from west to east or associated with modern riverine inputs throughout the basin. Despite the large-scale, long-term removal of fine-grained sediment from winnowed portions of the eastern Sound, the composition of the pollen and spore component of the sedimentary matrix conforms to a basin-wide homogeneous signal. These results strongly support the use of select regional palynological boundaries as chronostratigraphic tools to provide a framework for interpretation of the late glacial and Holocene history of the Long Island Sound basin sediments.

  8. Pollen tubes and the physical world.

    PubMed

    Winship, Lawrence J; Obermeyer, Gerhard; Geitmann, Anja; Hepler, Peter K

    2011-07-01

    The primary goal of our previous opinion paper (Winship, L.J. et al. (2010) Trends Plant Sci. 15, 363-369) [1] was to put two models for the control of pollen tube growth on the same theoretical and biophysical footing, and to then test both for consistency with basic principles and with experimental data. Our central thesis, then and now, is that the biophysical and biochemical mechanisms that enable pollen tubes to grow and to respond to their environment evolved in a physical context constrained by known, inescapable principles. First, pressure is a scalar, not a vector quantity. Second, the water movement in and out of plant cells that generates pressure is passive, not active, and is controlled by differences in water potential. Here we respond to the issues raised by Zonia and Munnik (Trends Plant Sci. 2011; this issue) [2] in the light of new evidence concerning turgor pressure and pollen tube growth rates. PMID:21536475

  9. Medicinal smoke reduces airborne bacteria.

    PubMed

    Nautiyal, Chandra Shekhar; Chauhan, Puneet Singh; Nene, Yeshwant Laxman

    2007-12-01

    This study represents a comprehensive analysis and scientific validation of our ancient knowledge about the effect of ethnopharmacological aspects of natural products' smoke for therapy and health care on airborne bacterial composition and dynamics, using the Biolog microplate panels and Microlog database. We have observed that 1h treatment of medicinal smoke emanated by burning wood and a mixture of odoriferous and medicinal herbs (havan sámagri=material used in oblation to fire all over India), on aerial bacterial population caused over 94% reduction of bacterial counts by 60 min and the ability of the smoke to purify or disinfect the air and to make the environment cleaner was maintained up to 24h in the closed room. Absence of pathogenic bacteria Corynebacterium urealyticum, Curtobacterium flaccumfaciens, Enterobacter aerogenes (Klebsiella mobilis), Kocuria rosea, Pseudomonas syringae pv. persicae, Staphylococcus lentus, and Xanthomonas campestris pv. tardicrescens in the open room even after 30 days is indicative of the bactericidal potential of the medicinal smoke treatment. We have demonstrated that using medicinal smoke it is possible to completely eliminate diverse plant and human pathogenic bacteria of the air within confined space. PMID:17913417

  10. Thunderstorm asthma due to grass pollen.

    PubMed

    Suphioglu, C

    1998-08-01

    It is widely known and accepted that grass pollen is a major outdoor cause of hay fever. Moreover, grass pollen is also responsible for triggering allergic asthma, gaining impetus as a result of the 1987/1989 Melbourne and 1994 London thunderstorm-associated asthma epidemics. However, grass pollen is too large to gain access into the lower airways to trigger the asthmatic response and micronic particles <5 micro m are required to trigger the response. We have successfully shown that ryegrass pollen ruptures upon contact with water, releasing about 700 starch granules which not only contain the major allergen Lol p 5, but have been shown to trigger both in vitro and in vivo IgE-mediated responses. Furthermore, starch granules have been isolated from the Melbourne atmosphere with 50-fold increase following rainfall. Free grass pollen allergen molecules have been recently shown to interact with other particles including diesel exhaust carbon particles, providing a further transport mechanism for allergens to gain access into lower airways. In this review, implication and evidence for grass pollen as a trigger of thunderstorm-associated asthma is presented. Such information is critical and mandatory for patient education and training in their allergen avoidance programs. More importantly, patients with serum IgE to group 5 allergens are at high risk of allergic asthma, especially those not protected by medication. Therefore, a system to determine the total atmospheric allergen load and devising of an effective asthma risk forecast is urgently needed and is subject to current investigation. PMID:9693274

  11. Airborne antenna pattern calculations

    NASA Technical Reports Server (NTRS)

    Knerr, T. J.; Schaffner, P. R.; Mielke, R. R.; Gilreath, M. C.

    1980-01-01

    A procedure for numerically calculating radiation patterns of fuselage-mounted airborne antennas using the Volumetric Pattern Analysis Program is presented. Special attention is given to aircraft modeling. An actual case study involving a large commercial aircraft is included to illustrate the analysis procedure.

  12. Recognizing Airborne Hazards.

    ERIC Educational Resources Information Center

    Schneider, Christian M.

    1990-01-01

    The heating, ventilating, and air conditioning (HVAC) systems in older buildings often do not adequately handle air-borne contaminants. Outlines a three-stage Indoor Air Quality (IAQ) assessment and describes a case in point at a Pittsburgh, Pennsylvania, school. (MLF)

  13. Airborne Fraunhofer Line Discriminator

    NASA Technical Reports Server (NTRS)

    Gabriel, F. C.; Markle, D. A.

    1969-01-01

    Airborne Fraunhofer Line Discriminator enables prospecting for fluorescent materials, hydrography with fluorescent dyes, and plant studies based on fluorescence of chlorophyll. Optical unit design is the coincidence of Fraunhofer lines in the solar spectrum occurring at the characteristic wavelengths of some fluorescent materials.

  14. Airborne Remote Sensing

    NASA Technical Reports Server (NTRS)

    1992-01-01

    NASA imaging technology has provided the basis for a commercial agricultural reconnaissance service. AG-RECON furnishes information from airborne sensors, aerial photographs and satellite and ground databases to farmers, foresters, geologists, etc. This service produces color "maps" of Earth conditions, which enable clients to detect crop color changes or temperature changes that may indicate fire damage or pest stress problems.

  15. Pollen-Climate Calibration, Characterization of Statistical Uncertainty, and Forward Modeling for Integration Into Bayesian Hierarchical Climate Reconstruction

    NASA Astrophysics Data System (ADS)

    Wahl, E. R.

    2008-12-01

    as much information as the multi-taxonomic MAT in well-selected regions, the ratio inverse model is used as the starting point for developing a temperature-pollen forward model. Such a forward model can be directly incorporated into a Bayesian hierarchical model (BHM) framework: the reason for seeking a taxonomically simplified pollen model is itself driven by computational considerations within the BHM. The BHM framework thus allows pollen- derived climate information to contribute multi-decadal to centennial information into a simultaneous reconstruction framework that also incorporates annually-resolved climate information from tree ring sources and centennial to multi-centennial information from borehole reconstructions. In the forward model, the pine and oak pollen counts are treated as binomial random variables with expected values (p) and (1-p), conditional on the calibration temperature values and the total counts (n) used to determine the ratios. Model parameters and (p) values have been estimated using a binomial logistic form of the generalized linear model. Comparison of the estimated conditional expected (p) values with the associated calibration ratio/temperature relationships shows good performance for the SW N American test case, with little (but non- 0) bias and good overall tracking (R2 = 0.79). (4) The results from (3) indicate that appropriate forward models can be developed for a suite of sites in North America with temporally dense, well-dated fossil pollen records over the past 2ka, and then incorporated into the BHM. Modeling results for the suite of sites will be presented.

  16. Composition of polyphenol and polyamide compounds in common ragweed (Ambrosia artemisiifolia L.) pollen and sub-pollen particles.

    PubMed

    Mihajlovic, Luka; Radosavljevic, Jelena; Burazer, Lidija; Smiljanic, Katarina; Cirkovic Velickovic, Tanja

    2015-01-01

    Phenolic composition of Ambrosia artemisiifolia L. pollen and sub-pollen particles (SPP) aqueous extracts was determined, using a novel extraction procedure. Total phenolic and flavonoid content was determined, as well as the antioxidative properties of the extract. Main components of water-soluble pollen phenolics are monoglycosides and malonyl-mono- and diglycosides of isorhamnetin, quercetin and kaempferol, while spermidine derivatives were identified as the dominant polyamides. SPP are similar in composition to pollen phenolics (predominant isorhamnetin and quercetin monoglycosides), but lacking small phenolic molecules (<450Da). Ethanol-based extraction protocol revealed one-third lower amount of total phenolics in SPP than in pollen. For the first time in any pollen species, SPP and pollen phenolic compositions were compared in detail, with an UHPLC/ESI-LTQ-Orbitrap-MS-MS approach, revealing the presence of spermidine derivatives in both SPP and pollen, not previously reported in Ambrosia species. PMID:25468540

  17. Effects of NO2 and Ozone on Pollen Allergenicity

    PubMed Central

    Frank, Ulrike; Ernst, Dieter

    2016-01-01

    This mini-review summarizes the available data of the air pollutants NO2 and ozone on allergenic pollen from different plant species, focusing on potentially allergenic components of the pollen, such as allergen content, protein release, IgE-binding, or protein modification. Various in vivo and in vitro studies on allergenic pollen are shown and discussed. PMID:26870080

  18. Diversity and conservation in maize pollen: Phenotypes and transcripts

    EPA Science Inventory

    In addition to its crucial role in seed production, pollen serves as a vector for gene flow between plant populations. Recently, pollen was identified as a mechanism for introduction of transgenes into non-transgenic populations. To investigate the genetic basis for pollen fitn...

  19. Pollen Biology of Ornamental Ginger (Hedychium spp. J. Koenig)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An improved in vitro pollen germination assay was developed to assess the viability of stored Hedychium pollen. The effect of polyethylene glycol (PEG) (10, 15, and 20% w/v) on pollen germination and tube growth was evaluated for H. longicornutum and two commercial Hedychium cultivars, ‘Orange Brush...

  20. Effects of NO2 and Ozone on Pollen Allergenicity.

    PubMed

    Frank, Ulrike; Ernst, Dieter

    2016-01-01

    This mini-review summarizes the available data of the air pollutants NO2 and ozone on allergenic pollen from different plant species, focusing on potentially allergenic components of the pollen, such as allergen content, protein release, IgE-binding, or protein modification. Various in vivo and in vitro studies on allergenic pollen are shown and discussed. PMID:26870080

  1. Hygroscopic weight gain of pollen grains from Juniperus species

    NASA Astrophysics Data System (ADS)

    Bunderson, Landon D.; Levetin, Estelle

    2015-05-01

    Juniperus pollen is highly allergenic and is produced in large quantities across Texas, Oklahoma, and New Mexico. The pollen negatively affects human populations adjacent to the trees, and since it can be transported hundreds of kilometers by the wind, it also affects people who are far from the source. Predicting and tracking long-distance transport of pollen is difficult and complex. One parameter that has been understudied is the hygroscopic weight gain of pollen. It is believed that juniper pollen gains weight as humidity increases which could affect settling rate of pollen and thus affect pollen transport. This study was undertaken to examine how changes in relative humidity affect pollen weight, diameter, and settling rate. Juniperus ashei, Juniperus monosperma, and Juniperus pinchotii pollen were applied to greased microscope slides and placed in incubation chambers under a range of temperature and humidity levels. Pollen on slides were weighed using an analytical balance at 2- and 6-h intervals. The size of the pollen was also measured in order to calculate settling rate using Stokes' Law. All pollen types gained weight as humidity increased. The greatest settling rate increase was exhibited by J. pinchotii which increased by 24 %.

  2. Pollen Germination--A Challenging and Educational Experiment.

    ERIC Educational Resources Information Center

    Tse, H. L. H.; Chan, G. Y. S.

    2001-01-01

    Summarizes the recent research on pollen germination and introduces some basic studies on pollen tube growth that can be conducted in a secondary school laboratory. Discusses the use of a light microscope and refrigerator to study pollen. (Contains 13 references.) (Author/YDS)

  3. Hygroscopic weight gain of pollen grains from Juniperus species.

    PubMed

    Bunderson, Landon D; Levetin, Estelle

    2015-05-01

    Juniperus pollen is highly allergenic and is produced in large quantities across Texas, Oklahoma, and New Mexico. The pollen negatively affects human populations adjacent to the trees, and since it can be transported hundreds of kilometers by the wind, it also affects people who are far from the source. Predicting and tracking long-distance transport of pollen is difficult and complex. One parameter that has been understudied is the hygroscopic weight gain of pollen. It is believed that juniper pollen gains weight as humidity increases which could affect settling rate of pollen and thus affect pollen transport. This study was undertaken to examine how changes in relative humidity affect pollen weight, diameter, and settling rate. Juniperus ashei, Juniperus monosperma, and Juniperus pinchotii pollen were applied to greased microscope slides and placed in incubation chambers under a range of temperature and humidity levels. Pollen on slides were weighed using an analytical balance at 2- and 6-h intervals. The size of the pollen was also measured in order to calculate settling rate using Stokes' Law. All pollen types gained weight as humidity increased. The greatest settling rate increase was exhibited by J. pinchotii which increased by 24 %. PMID:25008113

  4. International Symposium on Airborne Geophysics

    NASA Astrophysics Data System (ADS)

    Mogi, Toru; Ito, Hisatoshi; Kaieda, Hideshi; Kusunoki, Kenichiro; Saltus, Richard W.; Fitterman, David V.; Okuma, Shigeo; Nakatsuka, Tadashi

    2006-05-01

    Airborne geophysics can be defined as the measurement of Earth properties from sensors in the sky. The airborne measurement platform is usually a traditional fixed-wing airplane or helicopter, but could also include lighter-than-air craft, unmanned drones, or other specialty craft. The earliest history of airborne geophysics includes kite and hot-air balloon experiments. However, modern airborne geophysics dates from the mid-1940s when military submarine-hunting magnetometers were first used to map variations in the Earth's magnetic field. The current gamut of airborne geophysical techniques spans a broad range, including potential fields (both gravity and magnetics), electromagnetics (EM), radiometrics, spectral imaging, and thermal imaging.

  5. Isolation of Cytoplasmic Enzymes from Pollen 1

    PubMed Central

    Weeden, Norman F.; Gottlieb, Leslie D.

    1980-01-01

    The cytoplasmic isozyme of many cytoplasmic-organelle isozyme pairs, as well as other cytoplasmic enzymes in plants, can be readily obtained from pollen by soaking it in an appropriate buffer for 4 hours. Enzymes localized in subcellular organelles appear not to be released during the soaking period, although they are released if the pollen is crushed. The technique is a useful initial step in studies of subcellular localization of enzymes or for obtaining small quantities of cytoplasmic enzymes free of organellar contaminants. Images PMID:16661444

  6. textbf{Mesoscale simulations of pollen and agricultural soil dust containing bacteria as ice nucleators}

    NASA Astrophysics Data System (ADS)

    Hummel, M.; Hoose, C.; Steinke, I.; Jäger, S.; Oehm, C.; Möhler, O.; Vogel, H.; Schurgers, G.

    2012-04-01

    One of the greatest uncertainties in climate impacts is the one caused by atmospheric aerosols. This is because of the variety in aerosols as well as in their influences. Little is known about the contribution of primary biological aerosol particles (PBAP). This project focuses on PBAP influences on mixed phase clouds simulated with the regional atmospheric model COSMO-ART. PBAPs are directly emitted into the atmosphere from different sources and are pollen grains, fungal spores, bacteria or leaf fragments for example. A suggested influence of PBAPs on clouds consists in their ability to act as ice nuclei, which are required to initiate freezing of atmospheric cloud droplets at temperatures above homogeneous nucleation. In laboratory experiments some PBAPs have been identified as efficient ice nuclei at relatively warm temperatures. Here we present simulations including birch and pine pollen grains as well as agricultural soil dust containing bacteria. The emission fluxes are weighed with the individual coverage of birch or pine trees or cropland, respectively. For soil dust all cropland area is assumed to lie follow and is allowed to contribute to the emissions. Thus, this simulation is a sensitivity study to estimate the maximum possible contribution of dust particles from agricultural areas. The model domain includes most part of Europe with a horizontal resolution of 13.4 km and terrain-following model layers. The selected time period, beginning of April 2008, falls into the birch pollen season as determined from observed pollen counts. An empirical ice nucleation parameterization is implemented into the model, assuming that heterogeneous freezing follows the singular hypothesis. This implies that each particle has a distribution of actives sites depending on temperature. The ice active surface site densities are based on measurements for ice nucleation on soil dust and different pollen at the local AIDA chamber. In our simulations diagnostic ice nuclei from

  7. Pollen Lipidomics: Lipid Profiling Exposes a Notable Diversity in 22 Allergenic Pollen and Potential Biomarkers of the Allergic Immune Response

    PubMed Central

    Bashir, Mohamed Elfatih H.; Lui, Jan Hsi; Palnivelu, Ravishankar; Naclerio, Robert M.; Preuss, Daphne

    2013-01-01

    Background/Aim Pollen grains are the male gametophytes that deliver sperm cells to female gametophytes during sexual reproduction of higher plants. Pollen is a major source of aeroallergens and environmental antigens. The pollen coat harbors a plethora of lipids that are required for pollen hydration, germination, and penetration of the stigma by pollen tubes. In addition to proteins, pollen displays a wide array of lipids that interact with the human immune system. Prior searches for pollen allergens have focused on the identification of intracellular allergenic proteins, but have largely overlooked much of the extracellular pollen matrix, a region where the majority of lipid molecules reside. Lipid antigens have attracted attention for their potent immunoregulatory effects. By being in close proximity to allergenic proteins on the pollen surface when they interact with host cells, lipids could modify the antigenic properties of proteins. Methodology/Principal Findings We performed a comparative pollen lipid profiling of 22 commonly allergenic plant species by the use of gas chromatography-mass spectroscopy, followed by detailed data mining and statistical analysis. Three experiments compared pollen lipid profiles. We built a database library of the pollen lipids by matching acquired pollen-lipid mass spectra and retention times with the NIST/EPA/NIH mass-spectral library. We detected, identified, and relatively quantified more than 106 lipid molecular species including fatty acids, n-alkanes, fatty alcohols, and sterols. Pollen-derived lipids stimulation up-regulate cytokines expression of dendritic and natural killer T cells co-culture. Conclusions/Significance Here we report on a lipidomic analysis of pollen lipids that can serve as a database for identifying potential lipid antigens and/or novel candidate molecules involved in allergy. The database provides a resource that facilitates studies on the role of lipids in the immunopathogenesis of allergy. Pollen

  8. Sublattice counting and orbifolds

    NASA Astrophysics Data System (ADS)

    Hanany, Amihay; Orlando, Domenico; Reffert, Susanne

    2010-06-01

    Abelian orbifolds of mathbb{C}3 are known to be encoded by hexagonal brane tilings. To date it is not known how to count all such orbifolds. We fill this gap by employing number theoretic techniques from crystallography, and by making use of Polya's Enumeration Theorem. The results turn out to be beautifully encoded in terms of partition functions and Dirichlet series. The same methods apply to counting orbifolds of any toric non-compact Calabi-Yau singularity. As additional examples, we count the orbifolds of the conifold, of the L aba theories, and of mathbb{C}4.

  9. Arabidopsis FIMBRIN5, an Actin Bundling Factor, Is Required for Pollen Germination and Pollen Tube Growth[W

    PubMed Central

    Wu, Youjun; Yan, Jin; Zhang, Ruihui; Qu, Xiaolu; Ren, Sulin; Chen, Naizhi; Huang, Shanjin

    2010-01-01

    Actin cables in pollen tubes serve as molecular tracks for cytoplasmic streaming and organelle movement and are formed by actin bundling factors like villins and fimbrins. However, the precise mechanisms by which actin cables are generated and maintained remain largely unknown. Fimbrins comprise a family of five members in Arabidopsis thaliana. Here, we characterized a fimbrin isoform, Arabidopsis FIMBRIN5 (FIM5). Our results show that FIM5 is required for the organization of actin cytoskeleton in pollen grains and pollen tubes, and FIM5 loss-of-function associates with a delay of pollen germination and inhibition of pollen tube growth. FIM5 decorates actin filaments throughout pollen grains and tubes. Actin filaments become redistributed in fim5 pollen grains and disorganized in fim5 pollen tubes. Specifically, actin cables protrude into the extreme tips, and their longitudinal arrangement is disrupted in the shank of fim5 pollen tubes. Consequently, the pattern and velocity of cytoplasmic streaming were altered in fim5 pollen tubes. Additionally, loss of FIM5 function rendered pollen germination and tube growth hypersensitive to the actin-depolymerizing drug latrunculin B. In vitro biochemical analyses indicated that FIM5 exhibits actin bundling activity and stabilizes actin filaments. Thus, we propose that FIM5 regulates actin dynamics and organization during pollen germination and tube growth via stabilizing actin filaments and organizing them into higher-order structures. PMID:21098731

  10. Inventory count strategies.

    PubMed

    Springer, W H

    1996-02-01

    An important principle of accounting is that asset inventory needs to be correctly valued to ensure that the financial statements of the institution are accurate. Errors is recording the value of ending inventory in one fiscal year result in errors to published financial statements for that year as well as the subsequent fiscal year. Therefore, it is important that accurate physical counts be periodically taken. It is equally important that any system being used to generate inventory valuation, reordering or management reports be based on consistently accurate on-hand balances. At the foundation of conducting an accurate physical count of an inventory is a comprehensive understanding of the process coupled with a written plan. This article presents a guideline of the physical count processes involved in a traditional double-count approach. PMID:10165241

  11. Calorie count - Alcoholic beverages

    MedlinePlus

    ... want to watch how much you drink. Cocktails mixed with soda, cream, or ice cream can have especially high calorie counts. If you find you are having trouble cutting back on alcohol , talk with your doctor. Here is a list ...

  12. Blood Count Tests

    MedlinePlus

    Your blood contains red blood cells (RBC), white blood cells (WBC), and platelets. Blood count tests measure the number and types of cells in your blood. This helps doctors check on your overall health. ...

  13. Blood Count Tests

    MedlinePlus

    ... white blood cells (WBC), and platelets. Blood count tests measure the number and types of cells in ... helps doctors check on your overall health. The tests can also help to diagnose diseases and conditions ...

  14. Counting Knights and Knaves

    ERIC Educational Resources Information Center

    Levin,Oscar; Roberts, Gerri M.

    2013-01-01

    To understand better some of the classic knights and knaves puzzles, we count them. Doing so reveals a surprising connection between puzzles and solutions, and highlights some beautiful combinatorial identities.

  15. Pollen tetrads in the detection of environmental mutagenesis

    SciTech Connect

    Mulcahy, D.L.

    1981-01-01

    Although pollen is a very sensitive indicator of environmental mutagenesis, it is also sensitive to nonmutagenic environmental stress. By analyzing pollen tetrads, rather than individual pollen grains, it is possible to distinguish between mutagenic and nonmutagenic influences. Another advantage of using pollen tetrads in mutagenicity studies is that it is possible to discriminate between pre- and post-pachytene mutations. This eliminates the mutant sector problem of a single mutational event giving rise to a large number of mutant cells. Methods of analyzing pollen tetrads are described.

  16. Pollen wall development: the associated enzymes and metabolic pathways.

    PubMed

    Jiang, J; Zhang, Z; Cao, J

    2013-03-01

    Pollen grains are surrounded by a sculpted wall, which protects male gametophytes from various environmental stresses and microbial attacks, and also facilitates pollination. Pollen wall development requires lipid and polysaccharide metabolism, and some key genes and proteins that participate in these processes have recently been identified. Here, we summarise the genes and describe their functions during pollen wall development via several metabolic pathways. A working model involving substances and catalytic enzyme reactions that occur during pollen development is also presented. This model provides information on the complete process of pollen wall development with respect to metabolic pathways. PMID:23252839

  17. Extensive Pollen Flow but Few Pollen Donors and High Reproductive Variance in an Extremely Fragmented Landscape

    PubMed Central

    González-Martínez, Santiago C.; Aparicio, Abelardo

    2012-01-01

    Analysing pollen movement is a key to understanding the reproductive system of plant species and how it is influenced by the spatial distribution of potential mating partners in fragmented populations. Here we infer parameters related to levels of pollen movement and diversity of the effective pollen cloud for the wind-pollinated shrub Pistacia lentiscus across a highly disturbed landscape using microsatellite loci. Paternity analysis and the indirect KinDist and Mixed Effect Mating models were used to assess mating patterns, the pollen dispersal kernel, the effective number of males (Nep) and their relative individual fertility, as well as the existence of fine-scale spatial genetic structure in adult plants. All methods showed extensive pollen movement, with high rates of pollen flow from outside the study site (up to 73–93%), fat-tailed dispersal kernels and large average pollination distances (δ = 229–412 m). However, they also agreed in detecting very few pollen donors (Nep = 4.3–10.2) and a large variance in their reproductive success: 70% of males did not sire any offspring among the studied female plants and 5.5% of males were responsible for 50% of pollinations. Although we did not find reduced levels of genetic diversity, the adult population showed high levels of biparental inbreeding (14%) and strong spatial genetic structure (Sp = 0.012), probably due to restricted seed dispersal and scarce safe sites for recruitment. Overall, limited seed dispersal and the scarcity of successful pollen donors can be contributing to generate local pedigrees and to increase inbreeding, the prelude of genetic impoverishment. PMID:23152842

  18. The bias and signal attenuation present in conventional pollen-based climate reconstructions as assessed by early climate data from Minnesota, USA.

    PubMed

    St Jacques, Jeannine-Marie; Cumming, Brian F; Sauchyn, David J; Smol, John P

    2015-01-01

    The inference of past temperatures from a sedimentary pollen record depends upon the stationarity of the pollen-climate relationship. However, humans have altered vegetation independent of changes to climate, and consequently modern pollen deposition is a product of landscape disturbance and climate, which is different from the dominance of climate-derived processes in the past. This problem could cause serious signal distortion in pollen-based reconstructions. In the north-central United States, direct human impacts have strongly altered the modern vegetation and hence the pollen rain since Euro-American settlement in the mid-19th century. Using instrumental temperature data from the early 1800 s from Fort Snelling (Minnesota), we assessed the signal distortion and bias introduced by using the conventional method of inferring temperature from pollen assemblages in comparison to a calibration set from pre-settlement pollen assemblages and the earliest instrumental climate data. The early post-settlement calibration set provides more accurate reconstructions of the 19th century instrumental record, with less bias, than the modern set does. When both modern and pre-industrial calibration sets are used to reconstruct past temperatures since AD 1116 from pollen counts from a varve-dated record from Lake Mina, Minnesota, the conventional inference method produces significant low-frequency (centennial-scale) signal attenuation and positive bias of 0.8-1.7 °C, resulting in an overestimation of Little Ice Age temperature and likely an underestimation of the extent and rate of anthropogenic warming in this region. However, high-frequency (annual-scale) signal attenuation exists with both methods. Hence, we conclude that any past pollen spectra from before Euro-American settlement in this region should be interpreted using a pre-Euro-American settlement pollen set, paired to the earliest instrumental climate records. It remains to be explored how widespread this problem is

  19. The Bias and Signal Attenuation Present in Conventional Pollen-Based Climate Reconstructions as Assessed by Early Climate Data from Minnesota, USA

    PubMed Central

    St. Jacques, Jeannine-Marie; Cumming, Brian F.; Sauchyn, David J.; Smol, John P.

    2015-01-01

    The inference of past temperatures from a sedimentary pollen record depends upon the stationarity of the pollen-climate relationship. However, humans have altered vegetation independent of changes to climate, and consequently modern pollen deposition is a product of landscape disturbance and climate, which is different from the dominance of climate-derived processes in the past. This problem could cause serious signal distortion in pollen-based reconstructions. In the north-central United States, direct human impacts have strongly altered the modern vegetation and hence the pollen rain since Euro-American settlement in the mid-19th century. Using instrumental temperature data from the early 1800s from Fort Snelling (Minnesota), we assessed the signal distortion and bias introduced by using the conventional method of inferring temperature from pollen assemblages in comparison to a calibration set from pre-settlement pollen assemblages and the earliest instrumental climate data. The early post-settlement calibration set provides more accurate reconstructions of the 19th century instrumental record, with less bias, than the modern set does. When both modern and pre-industrial calibration sets are used to reconstruct past temperatures since AD 1116 from pollen counts from a varve-dated record from Lake Mina, Minnesota, the conventional inference method produces significant low-frequency (centennial-scale) signal attenuation and positive bias of 0.8-1.7°C, resulting in an overestimation of Little Ice Age temperature and likely an underestimation of the extent and rate of anthropogenic warming in this region. However, high-frequency (annual-scale) signal attenuation exists with both methods. Hence, we conclude that any past pollen spectra from before Euro-American settlement in this region should be interpreted using a pre-Euro-American settlement pollen set, paired to the earliest instrumental climate records. It remains to be explored how widespread this problem is

  20. Lipids in pollen - They are different.

    PubMed

    Ischebeck, Till

    2016-09-01

    During evolution, the male gametophyte of Angiosperms has been severely reduced to the pollen grain, consisting of a vegetative cell containing two sperm cells. This vegetative cell has to deliver the sperm cells from the stigma through the style to the ovule. It does so by producing a pollen tube and elongating it to many centimeters in length in some species, requiring vast amounts of fatty acid and membrane lipid synthesis. In order to optimize this polar tip growth, a unique lipid composition in the pollen has evolved. Pollen tubes produce extraplastidial galactolipids and store triacylglycerols in lipid droplets, probably needed as precursors of glycerolipids or for acyl editing. They also possess special sterol and sphingolipid moieties that might together form microdomains in the membranes. The individual lipid classes, the proteins involved in their synthesis as well as the corresponding Arabidopsis knockout mutant phenotypes are discussed in this review. This article is part of a Special Issue entitled: Plant Lipid Biology edited by Kent D. Chapman and Ivo Feussner. PMID:27033152

  1. The Beauty and Biology of Pollen.

    ERIC Educational Resources Information Center

    Clay-Poole, Scott T.; Slesnick, Irwin L.

    1983-01-01

    Describes: basic features of pollen grains (shapes, apertures, layering of wall, exine sculpturing); strategies for pollination (anemophily--wind transported, zoophily--animal transported); and the structures specialized for each process. Gives instructions for using scanning electron microscope photographs and for collecting, identifying, and…

  2. Pollen analyses of tarnished plant bugs

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Wild host plants play an important role for tarnished plant bug (TPB), Lygus lineolaris, especially when cultivated crops are not flowering. Knowledge of native habitats is important for managing this insect pest. Although pollen has been used to characterize dispersal and food sources of many inse...

  3. VEGETATION AND POLLEN RELATIONSHIP IN EASTERN CANADA

    EPA Science Inventory

    The relationship between the vegetation and modern pollen assemblages in eastern Canada is summarized and analyzed using isopoll maps, ordination, and cluster analysis. he major vegetation zones recognized in the region are the shrub tundra, forest tundra (divided into shrub and ...

  4. Ozone affects pollen viability and NAD(P)H oxidase release from Ambrosia artemisiifolia pollen.

    PubMed

    Pasqualini, Stefania; Tedeschini, Emma; Frenguelli, Giuseppe; Wopfner, Nicole; Ferreira, Fatima; D'Amato, Gennaro; Ederli, Luisa

    2011-10-01

    Air pollution is frequently proposed as a cause of the increased incidence of allergy in industrialised countries. We investigated the impact of ozone (O(3)) on reactive oxygen species (ROS) and allergen content of ragweed pollen (Ambrosia artemisiifolia). Pollen was exposed to acute O(3) fumigation, with analysis of pollen viability, ROS and nitric oxide (NO) content, activity of nicotinamide adenine dinucleotide phosphate (NAD[P]H) oxidase, and expression of major allergens. There was decreased pollen viability after O(3) fumigation, which indicates damage to the pollen membrane system, although the ROS and NO contents were not changed or were only slightly induced, respectively. Ozone exposure induced a significant enhancement of the ROS-generating enzyme NAD(P)H oxidase. The expression of the allergen Amb a 1 was not affected by O(3), determined from the mRNA levels of the major allergens. We conclude that O(3) can increase ragweed pollen allergenicity through stimulation of ROS-generating NAD(P)H oxidase. PMID:21605929

  5. Pollen Production, Microsporangium Dehiscence and Pollen Flow in Himalayan Cedar (Cedrus deodara Roxb. ex D. Don)

    PubMed Central

    KHANDURI, V. P.; SHARMA, C. M.

    2002-01-01

    Microsporangium dehiscence, pollen production and dispersal were studied in Himalayan cedar (Cedrus deodara) during 1998 and 1999. Microsporangium dehiscence showed diurnal periodicity and was found to be related to air temperature and relative air humidity, with a strobilus taking 2 d to dehisce completely in warmer conditions and 3 d in cooler ones. The frequency of flowering in C. deodara was highly variable during the two successive years; however, cyclical production of pollen grains was observed in 50 % of the trees. The maximum concentration of pollen grains in the air was found between 1200 and 1600 h, and this period was also noted to be the best time for pollination. Studying migration of pollen grains from isolated single trees in three directions showed that migration was not uniform in all directions. Long‐distance transport of pollen grains was observed in the downhill direction. However, in the uphill and horizontal directions grains could travel only up to 97·5 and 195·1 m, respectively, and the frequency of pollen grains to the source frequency at these distances was only 1·9 and 2·5 %, respectively. The results suggest that an isolation barrier of 190 m may be considered as a minimum for the management of deodar seed orchards. PMID:12099533

  6. Molecular Ice Nucleation Activity of Birch Pollen

    NASA Astrophysics Data System (ADS)

    Felgitsch, Laura; Bichler, Magdalena; Häusler, Thomas; Weiss, Victor U.; Marchetti-Deschmann, Martina; Allmaier, Günter; Grothe, Hinrich

    2015-04-01

    Heterogeneous ice nucleation plays a major part in ecosystem and climate. Due to the triggering of ice cloud formation it influences the radiation balance of the earth, but also on the ground it can be found to be important in many processes of nature. So far the process of heterogeneous ice nucleation is not fully understood and many questions remain to be answered. Biological ice nucleation is hereby from great interest, because it shows the highest freezing temperatures. Several bacteria and fungi act as ice nuclei. A famous example is Pseudomonas syringae, a bacterium in commercial use (Snomax®), which increases the freezing from homogeneous freezing temperatures of approx. -40° C (for small volumes as in cloud droplets) to temperatures up to -2° C. In 2001 it was found that birch pollen can trigger ice nucleation (Diehl et al. 2001; Diehl et al. 2002). For a long time it was believed that this is due to macroscopic features of the pollen surface. Recent findings of Bernhard Pummer (2012) show a different picture. The ice nuclei are not attached on the pollen surface directly, but on surface material which can be easily washed off. This shows that not only the surface morphology, but also specific molecules or molecular structures are responsible for the ice nucleation activity of birch pollen. With various analytic methods we work on elucidating the structure of these molecules as well as the mechanism with which they trigger ice nucleation. To solve this we use various instrumental analytic techniques like Nuclear Magnetic Resonance spectroscopy (NMR), Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS), and Gas-phase Electrophoretic Mobility Molecular Analysis (GEMMA). Also standard techniques like various chromatographic separation techniques and solvent extraction are in use. We state here that this feature might be due to the aggregation of small molecules, with agglomerates showing a specific surface structure. Our results

  7. Proteomic analyses of apoplastic proteins from germinating Arabidopsis thaliana pollen

    PubMed Central

    Ge, Weina; Song, Yun; Zhang, Cuijun; Zhang, Yafang; Burlingame, Alma L.; Guo, Yi

    2011-01-01

    Pollen grains play important roles in the reproductive processes of flowering plants. The roles of apoplastic proteins in pollen germination and in pollen tube growth are comparatively less well understood. To investigate the functions of apoplastic proteins in pollen germination, the global apoplastic proteins of mature and germinated Arabidopsis thaliana pollen grains were prepared for differential analyses by using 2-dimensional fluorescence difference gel electrophoresis (2-D DIGE) saturation labeling techniques. One hundred and three proteins differentially expressed (p value ≤ 0.01) in pollen germinated for 6h compare with un-germination mature pollen, and 98 spots, which represented 71 proteins, were identified by LC-MS/MS. By bioinformatics analysis, 50 proteins were identified as secreted proteins. These proteins were mainly involved in cell wall modification and remodeling, protein metabolism and signal transduction. Three of the differentially expressed proteins were randomly selected to determine their subcellular localizations by transiently expressing YFP fusion proteins. The results of subcellular localization were identical with the bioinformatics prediction. Based on these data, we proposed a model for apoplastic proteins functioning in pollen germination and pollen tube growth. These results will lead to a better understanding of the mechanisms of pollen germination and pollen tube growth. PMID:21798377

  8. Responses in the start of Betula (birch) pollen seasons to recent changes in spring temperatures across Europe.

    PubMed

    Emberlin, J; Detandt, M; Gehrig, R; Jaeger, S; Nolard, N; Rantio-Lehtimäki, A

    2002-09-01

    A shift in the timing of birch pollen seasons is important because it is well known to be a significant aeroallergen, especially in NW Europe where it is a notable cause of hay fever and pollen-related asthma. The research reported in this paper aims to investigate temporal patterns in the start dates of Betula (birch) pollen seasons at selected sites across Europe. In particular it investigates relationships between the changes in start dates and changes in spring temperatures over approximately the last 20 years. Daily birch pollen counts were used from Kevo, Turku, London, Brussels, Zurich and Vienna, for the core period from 1982 to 1999 and, in some cases, from 1970 to 2000. The sites represent a range of biogeographical situations from just within the Arctic Circle through to North West Maritime and Continental Europe. Pollen samples were taken with Hirst-type volumetric spore traps. Weather data were obtained from the sites nearest to the pollen traps. The timing of birch pollen seasons is known to depend mostly on a non-linear balance between the winter chilling required to break dormancy, and spring temperatures. Pollen start dates and monthly mean temperatures for January through to May were compiled to 5-year running means to examine trends. The start dates for the next 10 years were calculated from regression equations for each site, on the speculative basis that the current trends would continue. The analyses show regional contrasts. Kevo shows a marked trend towards cooler springs and later starts. If this continues the mean start date will become about 6 days later over the next 10 years. Turku exhibits cyclic patterns in start dates. A current trend towards earlier starts is expected to continue until 2007, followed by another fluctuation. London, Brussels, Zurich and Vienna show very similar patterns in the trends towards earlier start dates. If the trend continues the mean start dates at these sites will advance by about 6 days over the next 10

  9. Responses in the start of Betula (birch) pollen seasons to recent changes in spring temperatures across Europe

    NASA Astrophysics Data System (ADS)

    Emberlin, J.; Detandt, M.; Gehrig, R.; Jaeger, S.; Nolard, N.; Rantio-Lehtimäki, A.

    2002-07-01

    A shift in the timing of birch pollen seasons is important because it is well known to be a significant aeroallergen, especially in NW Europe where it is a notable cause of hay fever and pollen-related asthma. The research reported in this paper aims to investigate temporal patterns in the start dates of Betula (birch) pollen seasons at selected sites across Europe. In particular it investigates relationships between the changes in start dates and changes in spring temperatures over approximately the last 20 years. Daily birch pollen counts were used from Kevo, Turku, London, Brussels, Zurich and Vienna, for the core period from 1982 to 1999 and, in some cases, from 1970 to 2000. The sites represent a range of biogeographical situations from just within the Arctic Circle through to North West Maritime and Continental Europe. Pollen samples were taken with Hirst-type volumetric spore traps. Weather data were obtained from the sites nearest to the pollen traps. The timing of birch pollen seasons is known to depend mostly on a non-linear balance between the winter chilling required to break dormancy, and spring temperatures. Pollen start dates and monthly mean temperatures for January through to May were compiled to 5-year running means to examine trends. The start dates for the next 10 years were calculated from regression equations for each site, on the speculative basis that the current trends would continue. The analyses show regional contrasts. Kevo shows a marked trend towards cooler springs and later starts. If this continues the mean start date will become about 6 days later over the next 10 years. Turku exhibits cyclic patterns in start dates. A current trend towards earlier starts is expected to continue until 2007, followed by another fluctuation. London, Brussels, Zurich and Vienna show very similar patterns in the trends towards earlier start dates. If the trend continues the mean start dates at these sites will advance by about 6 days over the next 10

  10. [Air-borne disease].

    PubMed

    Lameiro Vilariño, Carmen; del Campo Pérez, Victor M; Alonso Bürger, Susana; Felpeto Nodar, Irene; Guimarey Pérez, Rosa; Pérez Alvarellos, Alberto

    2003-11-01

    Respiratory protection is a factor which worries nursing professionals who take care of patients susceptible of transmitting microorganisms through the air more as every day passes. This type of protection covers the use of surgical or hygienic masks against the transmission of infection by airborne drops to the use of highly effective masks or respirators against the transmission of airborne diseases such as tuberculosis or SARS, a recently discovered disease. The adequate choice of this protective device and its correct use are fundamental in order to have an effective protection for exposed personnel. The authors summarize the main protective respiratory devices used by health workers, their characteristics and degree of effectiveness, as well as the circumstances under which each device is indicated for use. PMID:14705591

  11. Measurement of airborne {sup 218}Po - A Bayesian approach

    SciTech Connect

    Groer, P.G.; Lo, Y.

    1996-12-01

    The standard mathematical treatment of the buildup and decay of airborne radionuclides on a filter paper uses the solutions of the so-called bateman equations adapted to the sampling process. The equations can be interpreted as differential equations for the expectation of an underlying stochastic process, which describes the random fluctuations in the accumulation and decay of the sampled radioactive atoms. The process for the buildup and decay of airborne {sup 218}Po can be characterized as an {open_quotes}immigration-death process{close_quotes} in the widely adopted, biologically based jargon. The probability distribution for the number of {sup 218}Po atoms, accumulated after sampling time t, is Poisson. We show that the distribution of the number of counts, registered by a detector with efficiency {epsilon} during a counting period T after the end of sampling, it also Poisson, with mean dependent on {epsilon},t,T, the flowrate and N{sub o}, the number of airborne {sup 218}Po atoms per unit volume. This Poisson distribution was used to construct the likelihood given the observed number of counts. After inversion with Bayes` Theorem we obtained the posterior density for N{sub o}. This density characterizes the remaining uncertainty about the measured under of {sup 218}Po atoms per unit volume of air. 6 refs., 3 figs., 1 tab.

  12. Measurement of airborne 218Po--a Bayesian approach.

    PubMed

    Groer, P G; Lo, Y

    1996-12-01

    The standard mathematical treatment of the buildup and decay of airborne radionuclides on a filter paper uses the solutions of the so-called Bateman equations adapted to the sampling process. These equations can be interpreted as differential equations for the expectation of an underlying stochastic process, which describes the random fluctuations in the accumulation and decay of the sampled radioactive atoms. The process for the buildup and decay of airborne 218Po can be characterized as an "immigration-death process" in the widely adopted, biologically based jargon. The probability distribution for the number of 218Po atoms, accumulated after sampling time t, is Poisson. We show that the distribution of the number of counts, registered by a detector with efficiency epsilon during a counting period T after the end of sampling, is also Poisson, with mean dependent on epsilon, t, T, the flowrate and N(o), the number of airborne 218Po atoms per unit volume. This Poisson distribution was used to construct the likelihood given the observed number of counts. After inversion with Bayes' Theorem we obtained the posterior density for N(o). This density characterizes the remaining uncertainty about the measured number of 218Po atoms per unit volume of air. PMID:8919080

  13. Germination of stress-tolerant Eucalyptus pollen.

    PubMed

    Heslop-Harrison, J; Heslop-Harrison, Y

    1985-02-01

    Earlier reports have indicated that the pollen of Eucalyptus is mechanically robust and unusually resistant to the osmotic stress imposed by immersion in water. We have investigated some of the features of the germination mechanism in the pollen of E. rhodantha with a view to clarifying the role of pollen-wall specializations in determining this resistance. Cultured in vitro, the pollen showed erratic germination, with a scatter of germination times up to 24 h. This was associated with variation between individual grains in the rate of hydration and dispersal of the pectins of the oncus, the thickened outer component of the intine present at each aperture. The oncus is itself differentiated, with a refractive outer layer lying within a sporopollenin operculum and itself overlying the protein-bearing layer of the intine. The outer layer, interpreted as a compacted pectin, undergoes only slow dissolution in aqueous media after the lifting of the operculum, and it is this that apparently protects the grain from the effects of short-term osmotic stress. The rate of dissolution varies between grains, possibly as a consequence of minor differences in developmental rate in the final stages of differentiation in the anther, and this contributes to the wider scatter of germination times. The dehydrated pollen gave one-third of the potential germination after 24 h exposure to 60 degrees C, and a small proportion survived 24 h at 70 degrees C. This degree of heat tolerance must primarily reflect properties of the protoplast of the vegetative cell, not examined in the present study; but the wall specializations may well provide a guard against extreme desiccation, and it is noteworthy that the function of the germination mechanism is not prejudiced by exposure to high temperatures. PMID:4019590

  14. Improvement in the accuracy of back trajectories using WRF to identify pollen sources in southern Iberian Peninsula.

    PubMed

    Hernández-Ceballos, M A; Skjøth, C A; García-Mozo, H; Bolívar, J P; Galán, C

    2014-12-01

    Airborne pollen transport at micro-, meso-gamma and meso-beta scales must be studied by atmospheric models, having special relevance in complex terrain. In these cases, the accuracy of these models is mainly determined by the spatial resolution of the underlying meteorological dataset. This work examines how meteorological datasets determine the results obtained from atmospheric transport models used to describe pollen transport in the atmosphere. We investigate the effect of the spatial resolution when computing backward trajectories with the HYSPLIT model. We have used meteorological datasets from the WRF model with 27, 9 and 3 km resolutions and from the GDAS files with 1° resolution. This work allows characterizing atmospheric transport of Olea pollen in a region with complex flows. The results show that the complex terrain affects the trajectories and this effect varies with the different meteorological datasets. Overall, the change from GDAS to WRF-ARW inputs improves the analyses with the HYSPLIT model, thereby increasing the understanding the pollen episode. The results indicate that a spatial resolution of at least 9 km is needed to simulate atmospheric flows that are considerable affected by the relief of the landscape. The results suggest that the appropriate meteorological files should be considered when atmospheric models are used to characterize the atmospheric transport of pollen on micro-, meso-gamma and meso-beta scales. Furthermore, at these scales, the results are believed to be generally applicable for related areas such as the description of atmospheric transport of radionuclides or in the definition of nuclear-radioactivity emergency preparedness. PMID:24705823

  15. Airborne forest fire research

    NASA Technical Reports Server (NTRS)

    Mattingly, G. S.

    1974-01-01

    The research relating to airborne fire fighting systems is reviewed to provide NASA/Langley Research Center with current information on the use of aircraft in forest fire operations, and to identify research requirements for future operations. A literature survey, interview of forest fire service personnel, analysis and synthesis of data from research reports and independent conclusions, and recommendations for future NASA-LRC programs are included.

  16. MLS airborne antenna research

    NASA Technical Reports Server (NTRS)

    Yu, C. L.; Burnside, W. D.

    1975-01-01

    The geometrical theory of diffraction was used to analyze the elevation plane pattern of on-aircraft antennas. The radiation patterns for basic elements (infinitesimal dipole, circumferential and axial slot) mounted on fuselage of various aircrafts with or without radome included were calculated and compared well with experimental results. Error phase plots were also presented. The effects of radiation patterns and error phase plots on the polarization selection for the MLS airborne antenna are discussed.

  17. Drought, pollen and nectar availability, and pollination success.

    PubMed

    Waser, Nickolas M; Price, Mary V

    2016-06-01

    Pollination success of animal-pollinated flowers depends on rate of pollinator visits and on pollen deposition per visit, both of which should vary with the pollen and nectar "neighborhoods" of a plant, i.e., with pollen and nectar availability in nearby plants. One determinant of these neighborhoods is per-flower production of pollen and nectar, which is likely to respond to environmental influences. In this study, we explored environmental effects on pollen and nectar production and on pollination success in order to follow up a surprising result from a previous study: flowers of Ipomopsis aggregata received less pollen in years of high visitation by their hummingbird pollinators. A new analysis of the earlier data indicated that high bird visitation corresponded to drought years. We hypothesized that drought might contribute to the enigmatic prior result if it decreases both nectar and pollen production: in dry years, low nectar availability could cause hummingbirds to visit flowers at a higher rate, and low pollen availability could cause them to deposit less pollen per visit. A greenhouse experiment demonstrated that drought does reduce both pollen and nectar production by I. aggregata flowers. This result was corroborated across 6 yr of variable precipitation and soil moisture in four unmanipulated field populations. In addition, experimental removal of pollen from flowers reduced the pollen received by nearby flowers. We conclude that there is much to learn about how abiotic and biotic environmental drivers jointly affect pollen and nectar production and availability, and how this contributes to pollen and nectar neighborhoods and thus influences pollination success. PMID:27459771

  18. Maize pollen is an important allergen in occupationally exposed workers

    PubMed Central

    2011-01-01

    Background The work- or environmental-related type I sensitization to maize pollen is hardly investigated. We sought to determine the prevalence of sensitization to maize pollen among exposed workers and to identify the eliciting allergens. Methods In July 2010, 8 out of 11 subjects were examined who were repeatedly exposed to maize pollen by pollinating maize during their work in a biological research department. All 8 filled in a questionnaire and underwent skin prick testing (SPT) and immune-specific analyses. Results 5 out of the 8 exposed subjects had repeatedly suffered for at least several weeks from rhinitis, 4 from conjunctivitis, 4 from urticaria, and 2 from shortness of breath upon occupational exposure to maize pollen. All symptomatic workers had specific IgE antibodies against maize pollen (CAP class ≥ 1). Interestingly, 4 of the 5 maize pollen-allergic subjects, but none of the 3 asymptomatic exposed workers had IgE antibodies specific for grass pollen. All but one of the maize pollen-allergic subjects had suffered from allergic grass pollen-related symptoms for 6 to 11 years before job-related exposure to maize pollen. Lung function testing was normal in all cases. In immunoblot analyses, the allergenic components could be identified as Zea m 1 and Zea m 13. The reactivity is mostly caused by cross-reactivity to the homologous allergens in temperate grass pollen. Two sera responded to Zea m 3, but interestingly not to the corresponding timothy allergen indicating maize-specific IgE reactivity. Conclusion The present data suggest that subjects pollinating maize are at high risk of developing an allergy to maize pollen as a so far underestimated source of occupational allergens. For the screening of patients with suspected maize pollen sensitization, the determination of IgE antibodies specific for maize pollen is suitable. PMID:22165847

  19. Airborne field strength monitoring

    NASA Astrophysics Data System (ADS)

    Bredemeyer, J.; Kleine-Ostmann, T.; Schrader, T.; Münter, K.; Ritter, J.

    2007-06-01

    In civil and military aviation, ground based navigation aids (NAVAIDS) are still crucial for flight guidance even though the acceptance of satellite based systems (GNSS) increases. Part of the calibration process for NAVAIDS (ILS, DME, VOR) is to perform a flight inspection according to specified methods as stated in a document (DOC8071, 2000) by the International Civil Aviation Organization (ICAO). One major task is to determine the coverage, or, in other words, the true signal-in-space field strength of a ground transmitter. This has always been a challenge to flight inspection up to now, since, especially in the L-band (DME, 1GHz), the antenna installed performance was known with an uncertainty of 10 dB or even more. In order to meet ICAO's required accuracy of ±3 dB it is necessary to have a precise 3-D antenna factor of the receiving antenna operating on the airborne platform including all losses and impedance mismatching. Introducing precise, effective antenna factors to flight inspection to achieve the required accuracy is new and not published in relevant papers yet. The authors try to establish a new balanced procedure between simulation and validation by airborne and ground measurements. This involves the interpretation of measured scattering parameters gained both on the ground and airborne in comparison with numerical results obtained by the multilevel fast multipole algorithm (MLFMA) accelerated method of moments (MoM) using a complex geometric model of the aircraft. First results will be presented in this paper.

  20. Mutagenicity of airborne particles.

    PubMed

    Chrisp, C E; Fisher, G L

    1980-09-01

    The physical and chemical properties of airborne particles are important for the interpretation of their potential biologic significance as genotoxic hazards. For polydisperse particle size distributions, the smallest, most respirable particles are generally the most mutagenic. Particulate collection for testing purposes should be designed to reduce artifact formation and allow condensation of mutagenic compounds. Other critical factors such as UV irradiation, wind direction, chemical reactivity, humidity, sample storage, and temperature of combustion are important. Application of chemical extraction methods and subsequent class fractionation techniques influence the observed mutagenic activity. Particles from urban air, coal fly ash, automobile and diesel exhaust, agricultural burning and welding fumes contain primarily direct-acting mutagens. Cigarette smoke condensate, smoke from charred meat and protein pyrolysates, kerosene soot and cigarette smoke condensates contain primarily mutagens which require metabolic activation. Fractionation coupled with mutagenicity testing indicates that the most potent mutagens are found in the acidic fractions of urban air, coal fly ash, and automobile diesel exhaust, whereas mutagens in rice straw smoke and cigarette smoke condensate are found primarily in the basic fractions. The interaction of the many chemical compounds in complex mixtures from airborne particles is likely to be important in determining mutagenic or comutagenic potentials. Because the mode of exposure is generally frequent and prolonged, the presence of tumor-promoting agents in complex mixtures may be a major factor in evaluation of the carcinogenic potential of airborne particles. PMID:7005667

  1. Airborne wireless communication systems, airborne communication methods, and communication methods

    DOEpatents

    Deaton, Juan D.; Schmitt, Michael J.; Jones, Warren F.

    2011-12-13

    An airborne wireless communication system includes circuitry configured to access information describing a configuration of a terrestrial wireless communication base station that has become disabled. The terrestrial base station is configured to implement wireless communication between wireless devices located within a geographical area and a network when the terrestrial base station is not disabled. The circuitry is further configured, based on the information, to configure the airborne station to have the configuration of the terrestrial base station. An airborne communication method includes answering a 911 call from a terrestrial cellular wireless phone using an airborne wireless communication system.

  2. Development of personal pollen information—the next generation of pollen information and a step forward for hay fever sufferers

    NASA Astrophysics Data System (ADS)

    Kmenta, Maximilian; Bastl, Katharina; Jäger, Siegfried; Berger, Uwe

    2014-10-01

    Pollen allergies affect a large part of the European population and are considered likely to increase. User feedback indicates that there are difficulties in providing proper information and valid forecasts using traditional methods of aerobiology due to a variety of factors. Allergen content, pollen loads, and pollen allergy symptoms vary per region and year. The first steps in challenging such issues have already been undertaken. A personalized pollen-related symptom forecast is thought to be a possible answer. However, attempts made thus far have not led to an improvement in daily forecasting procedures. This study describes a model that was launched in 2013 in Austria to provide the first available personal pollen information. This system includes innovative forecast models using bi-hourly pollen data, traditional pollen forecasts based on historical data, meteorological data, and recent symptom data from the patient's hayfever diary. Furthermore, it calculates the personal symptom load in real time, in particular, the entries of the previous 5 days, to classify users. The personal pollen information was made available in Austria on the Austrian pollen information website and via a mobile pollen application, described herein for the first time. It is supposed that the inclusion of personal symptoms will lead to major improvements in pollen information concerning hay fever sufferers.

  3. Development of personal pollen information-the next generation of pollen information and a step forward for hay fever sufferers.

    PubMed

    Kmenta, Maximilian; Bastl, Katharina; Jäger, Siegfried; Berger, Uwe

    2014-10-01

    Pollen allergies affect a large part of the European population and are considered likely to increase. User feedback indicates that there are difficulties in providing proper information and valid forecasts using traditional methods of aerobiology due to a variety of factors. Allergen content, pollen loads, and pollen allergy symptoms vary per region and year. The first steps in challenging such issues have already been undertaken. A personalized pollen-related symptom forecast is thought to be a possible answer. However, attempts made thus far have not led to an improvement in daily forecasting procedures. This study describes a model that was launched in 2013 in Austria to provide the first available personal pollen information. This system includes innovative forecast models using bi-hourly pollen data, traditional pollen forecasts based on historical data, meteorological data, and recent symptom data from the patient's hayfever diary. Furthermore, it calculates the personal symptom load in real time, in particular, the entries of the previous 5 days, to classify users. The personal pollen information was made available in Austria on the Austrian pollen information website and via a mobile pollen application, described herein for the first time. It is supposed that the inclusion of personal symptoms will lead to major improvements in pollen information concerning hay fever sufferers. PMID:24357491

  4. Airborne Submillimeter Spectroscopy

    NASA Technical Reports Server (NTRS)

    Zmuidzinas, J.

    1998-01-01

    This is the final technical report for NASA-Ames grant NAG2-1068 to Caltech, entitled "Airborne Submillimeter Spectroscopy", which extended over the period May 1, 1996 through January 31, 1998. The grant was funded by the NASA airborne astronomy program, during a period of time after the Kuiper Airborne Observatory was no longer operational. Instead. this funding program was intended to help develop instrument concepts and technology for the upcoming SOFIA (Stratospheric Observatory for Infrared Astronomy) project. SOFIA, which is funded by NASA and is now being carried out by a consortium lead by USRA (Universities Space Research Association), will be a 747 aircraft carrying a 2.5 meter diameter telescope. The purpose of our grant was to fund the ongoing development of sensitive heterodyne receivers for the submillimeter band (500-1200 GHz), using sensitive superconducting (SIS) detectors. In 1997 July we submitted a proposal to USRA to construct a heterodyne instrument for SOFIA. Our proposal was successful [1], and we are now continuing our airborne astronomy effort with funding from USRA. A secondary purpose of the NAG2-1068 grant was to continue the anaIN'sis of astronomical data collected with an earlier instrument which was flown on the NASA Kuiper Airborne Observatory (KAO). The KAO instrument and the astronomical studies which were carried out with it were supported primarily under another grant, NAG2-744, which extended over October 1, 1991 through Januarv 31, 1997. For a complete description of the astronomical data and its anailysis, we refer the reader to the final technical report for NAG2-744, which was submitted to NASA on December 1. 1997. Here we report on the SIS detector development effort for SOFIA carried out under NAG2-1068. The main result of this effort has been the demonstration of SIS mixers using a new superconducting material niobium titanium nitride (NbTiN), which promises to deliver dramatic improvements in sensitivity in the 700

  5. Fast counting electronics for neutron coincidence counting

    DOEpatents

    Swansen, James E.

    1987-01-01

    An amplifier-discriminator is tailored to output a very short pulse upon an above-threshold input from a detector which may be a .sup.3 He detector. The short pulse output is stretched and energizes a light emitting diode (LED) to provide a visual output of operation and pulse detection. The short pulse is further fed to a digital section for processing and possible ORing with other like generated pulses. Finally, the output (or ORed output ) is fed to a derandomizing buffer which converts the rapidly and randomly occurring pulses into synchronized and periodically spaced-apart pulses for the accurate counting thereof. Provision is also made for the internal and external disabling of each individual channel of amplifier-discriminators in an ORed plurality of same.

  6. Fast counting electronics for neutron coincidence counting

    DOEpatents

    Swansen, J.E.

    1985-03-05

    An amplifier-discriminator is tailored to output a very short pulse upon an above-threshold input from a detector which may be a /sup 3/He detector. The short pulse output is stretched and energizes a light emitting diode (LED) to provide a visual output of operation and pulse detection. The short pulse is further fed to a digital section for processing and possible ORing with other like generated pulses. Finally, the output (or ORed output) is fed to a derandomizing buffer which converts the rapidly and randomly occurring pulses into synchronized and periodically spaced-apart pulses for the accurate counting thereof. Provision is also made for the internal and external disabling of each individual channel of amplifier-discriminators in an ORed plurality of same.

  7. Terrigenous fluxes of pollen, insect scale and land plant palynodebris observed by sediment traps deployed in the subarctic Pacific

    NASA Astrophysics Data System (ADS)

    Tsutsui, H.; Takahashi, K.; Fowell, S. J.; Matsuoka, K.; Jordan, R. W.; Yamamoto, S.

    2014-12-01

    From 1990 to 2009, sediment traps in the subarctic Pacific (SA; 49°N, 174°W) were deployed and recovered during each summer, allowing the long-term observation of particle fluxes. As the Pacific Decadal Oscillation index changed in 1999 as air-temp cooled, this study focused on pollen, land plant debris and insect scale fluxes at SA during 1998 to 2006. The max pollen and fern spores flux was a mean of 74 grains m2 d-1, and the following details: 65% of the total pollen counts represented by wind-pollinated trees (e.g., alder, birch and pine), 24% by the herbaceous plants (as herbs), and 11% by fern spores. Spore, herbaceous and wind-pollinated tree pollen (as wind-pollen) fluxes peaked in May and Sep-Oct, but flux peaks of the latter also occurred in April and Jun. The annual flux peaks of insect scales (of unknown origin) and land-plant debris were in May and Sep, but over the entire study period the max insect scale flux of 161 was in Aug 2002, with a mean of 16 scales m2d-1, while the max (in Aug 2004) and mean land-plant debris fluxes were 107 and 10 plant fragments m2d-1, respectively. The sediment traps are situated both side of the Aleutian Is., where snow and ice occurs from Oct to May. The ice-snow season accounts for 25% of the total annual particle flux in SA trap, with 75% throughout the rest of the year. The correlation coefficient among pollen, insect scales and land plant debris are: 1) 0.58 (p<1%) between wind-pollen and insect scales, 2) 0.75 (p<5%) between herb-pollen and land plant debris, 3) but only 0.14 between insect scales and herbaceous pollen. Thus, the production location, residence time, route and mode of transport of the particles are important factors. Normally, the wind-pollinated tree flowering season in the northern part of Alaska and Japan where are an upper stream to the stations is from Apr to Jun, with the pollen usually transported across the ocean by winds. Assuming that the pollen takes several months to arrive SA, the

  8. Whose interests count?

    PubMed

    Brudney, Daniel; Lantos, John D

    2014-10-01

    Whose interests should count and how should various interests be balanced at the pediatric patient's bedside? The interests of the child patient clearly count. Recently, however, many authors have argued that the family's interests also count. But how should we think about the interests of others? What does it mean to talk about "the family" in this context? Does it really just mean the interests of each individual family member? Or is the family itself a moral entity that has interests of its own independent of the interests of each of its members? Are such interests important only as they affect the patient's interest or also for their own sake? In this special supplement to Pediatrics, a group of pediatricians, philosophers, and lawyers grapple with these questions. They examine these issues from different angles and reach different conclusions. Jointly, they demonstrate the ethical importance and, above all, the ethical complexity of the family's role at the bedside. PMID:25274878

  9. Photon counting digital holography

    NASA Astrophysics Data System (ADS)

    Demoli, Nazif; Skenderović, Hrvoje; Stipčević, Mario; Pavičić, Mladen

    2016-05-01

    Digital holography uses electronic sensors for hologram recording and numerical method for hologram reconstruction enabling thus the development of advanced holography applications. However, in some cases, the useful information is concealed in a very wide dynamic range of illumination intensities and successful recording requires an appropriate dynamic range of the sensor. An effective solution to this problem is the use of a photon-counting detector. Such detectors possess counting rates of the order of tens to hundreds of millions counts per second, but conditions of recording holograms have to be investigated in greater detail. Here, we summarize our main findings on this problem. First, conditions for optimum recording of digital holograms for detecting a signal significantly below detector's noise are analyzed in terms of the most important holographic measures. Second, for time-averaged digital holograms, optimum recordings were investigated for exposures shorter than the vibration cycle. In both cases, these conditions are studied by simulations and experiments.

  10. The Quaternary fossil-pollen record and global change

    SciTech Connect

    Grimm, E.C. . Research and Collections Center)

    1993-03-01

    Fossil pollen provide one of the most valuable records of vegetation and climate change during the recent geological past. Advantages of the fossil-pollen record are that deposits containing fossil pollen are widespread, especially in areas having natural lakes, that fossil pollen occurs in continuous stratigraphic sequences spanning millennia, and that fossil pollen occurs in quantitative assemblages permitting a multivariate approach for reconstructing past vegetation and climates. Because of stratigraphic continuity, fossil pollen records climate cycles on a wide range of scales, from annual to the 100 ka Milankovitch cycles. Receiving particular emphasis recently are decadal to century scale changes, possible from the sediments of varved lakes, and late Pleistocene events on a 5--10 ka scale possibly correlating with the Heinrich events in the North Atlantic marine record or the Dansgaard-Oeschger events in the Greenland ice-core record. Researchers have long reconstructed vegetation and climate by qualitative interpretation of the fossil-pollen record. Recently quantitative interpretation has developed with the aid of large fossil-pollen databases and sophisticated numerical models. In addition, fossil pollen are important climate proxy data for validating General Circulation Models, which are used for predicting the possible magnitude future climate change. Fossil-pollen data also contribute to an understanding of ecological issues associated with global climate change, including questions of how and how rapidly ecosystems might respond to abrupt climate change.

  11. Cell Wall Composition, Biosynthesis and Remodeling during Pollen Tube Growth

    PubMed Central

    Mollet, Jean-Claude; Leroux, Christelle; Dardelle, Flavien; Lehner, Arnaud

    2013-01-01

    The pollen tube is a fast tip-growing cell carrying the two sperm cells to the ovule allowing the double fertilization process and seed setting. To succeed in this process, the spatial and temporal controls of pollen tube growth within the female organ are critical. It requires a massive cell wall deposition to promote fast pollen tube elongation and a tight control of the cell wall remodeling to modify the mechanical properties. In addition, during its journey, the pollen tube interacts with the pistil, which plays key roles in pollen tube nutrition, guidance and in the rejection of the self-incompatible pollen. This review focuses on our current knowledge in the biochemistry and localization of the main cell wall polymers including pectin, hemicellulose, cellulose and callose from several pollen tube species. Moreover, based on transcriptomic data and functional genomic studies, the possible enzymes involved in the cell wall remodeling during pollen tube growth and their impact on the cell wall mechanics are also described. Finally, mutant analyses have permitted to gain insight in the function of several genes involved in the pollen tube cell wall biosynthesis and their roles in pollen tube growth are further discussed. PMID:27137369

  12. Role of Lipid Metabolism in Plant Pollen Exine Development.

    PubMed

    Zhang, Dabing; Shi, Jianxin; Yang, Xijia

    2016-01-01

    Pollen plays important roles in the life cycle of angiosperms plants. It acts as not only a biological protector of male sperms but also a communicator between the male and the female reproductive organs, facilitating pollination and fertilization. Pollen is produced within the anther, and covered by the specialized outer envelope, pollen wall. Although the morphology of pollen varies among different plant species, the pollen wall is mainly comprised of three layers: the pollen coat, the outer exine layer, and the inner intine layer. Except the intine layer, the other two layers are basically of lipidic nature. Particularly, the outer pollen wall layer, the exine, is a highly resistant biopolymer of phenylpropanoid and lipidic monomers covalently coupled by ether and ester linkages. The precise molecular mechanisms underlying pollen coat formation and exine patterning remain largely elusive. Herein, we summarize the current genetic, phenotypic and biochemical studies regarding to the pollen exine development and underlying molecular regulatory mechanisms mainly obtained from monocot rice (Oryza sativa) and dicot Arabidopsis thaliana, aiming to extend our understandings of plant male reproductive biology. Genes, enzymes/proteins and regulatory factors that appear to play conserved and diversified roles in lipid biosynthesis, transportation and modification during pollen exine formation, were highlighted. PMID:27023241

  13. Anther evolution: pollen presentation strategies when pollinators differ.

    PubMed

    Castellanos, Maria Clara; Wilson, Paul; Keller, Sarah J; Wolfe, Andrea D; Thomson, James D

    2006-02-01

    Male-male competition in plants is thought to exert selection on flower morphology and on the temporal presentation of pollen. Theory suggests that a plant's pollen dosing strategy should evolve to match the abundance and pollen transfer efficiency of its pollinators. Simultaneous pollen presentation should be favored when pollinators are infrequent or efficient at delivering the pollen they remove, whereas gradual dosing should optimize delivery by frequent and wasteful pollinators. Among Penstemon and Keckiella species, anthers vary in ways that affect pollen release, and the morphology of dried anthers reliably indicates how they dispense pollen. In these genera, hummingbird pollination has evolved repeatedly from hymenopteran pollination. Pollen production does not change with evolutionary shifts between pollinators. We show that after we control for phylogeny, hymenopteran-adapted species present their pollen more gradually than hummingbird-adapted relatives. In a species pair that seemed to defy the pattern, the rhythm of anther maturation produced an equivalent dosing effect. These results accord with previous findings that hummingbirds can be more efficient than bees at delivering pollen. PMID:16670987

  14. Differences in grass pollen allergen exposure across Australia

    PubMed Central

    Beggs, Paul J.; Katelaris, Constance H.; Medek, Danielle; Johnston, Fay H.; Burton, Pamela K.; Campbell, Bradley; Jaggard, Alison K.; Vicendese, Don; Bowman, David M.J.S.; Godwin, Ian; Huete, Alfredo R.; Erbas, Bircan; Green, Brett J.; Newnham, Rewi M.; Newbigin, Ed; Haberle, Simon G.; Davies, Janet M.

    2015-01-01

    Objective Allergic rhinitis and allergic asthma are important chronic diseases posing serious public health issues in Australia with associated medical, economic, and societal burdens. Pollen are significant sources of clinically relevant outdoor aeroallergens, recognised as both a major trigger for, and cause of, allergic respiratory diseases. This study aimed to provide a national, and indeed international, perspective on the state of Australian pollen data using a large representative sample. Methods Atmospheric grass pollen concentration is examined over a number of years within the period 1995 to 2013 for Brisbane, Canberra, Darwin, Hobart, Melbourne, and Sydney, including determination of the ‘clinical’ grass pollen season and grass pollen peak. Results The results of this study describe, for the first time, a striking spatial and temporal variability in grass pollen seasons in Australia, with important implications for clinicians and public health professionals, and the Australian grass pollen-allergic community. Conclusions These results demonstrate that static pollen calendars are of limited utility and in some cases misleading. This study also highlights significant deficiencies and limitations in the existing Australian pollen monitoring and data. Implications Establishment of an Australian national pollen monitoring network would help facilitate advances in the clinical and public health management of the millions of Australians with asthma and allergic rhinitis. PMID:25648730

  15. PECTIN METHYLESTERASE48 is involved in Arabidopsis pollen grain germination.

    PubMed

    Leroux, Christelle; Bouton, Sophie; Kiefer-Meyer, Marie-Christine; Fabrice, Tohnyui Ndinyanka; Mareck, Alain; Guénin, Stéphanie; Fournet, Françoise; Ringli, Christoph; Pelloux, Jérôme; Driouich, Azeddine; Lerouge, Patrice; Lehner, Arnaud; Mollet, Jean-Claude

    2015-02-01

    Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated the role of PECTIN METHYLESTERASE48 (PME48), an enzyme implicated in the remodeling of pectins in Arabidopsis (Arabidopsis thaliana) pollen. A combination of functional genomics, gene expression, in vivo and in vitro pollen germination, immunolabeling, and biochemical analyses was used on wild-type and Atpme48 mutant plants. We showed that AtPME48 is specifically expressed in the male gametophyte and is the second most expressed PME in dry and imbibed pollen grains. Pollen grains from homozygous mutant lines displayed a significant delay in imbibition and germination in vitro and in vivo. Moreover, numerous pollen grains showed two tips emerging instead of one in the wild type. Immunolabeling and Fourier transform infrared analyses showed that the degree of methylesterification of the homogalacturonan was higher in pme48-/- pollen grains. In contrast, the PME activity was lower in pme48-/-, partly due to a reduction of PME48 activity revealed by zymogram. Interestingly, the wild-type phenotype was restored in pme48-/- with the optimum germination medium supplemented with 2.5 mm calcium chloride, suggesting that in the wild-type pollen, the weakly methylesterified homogalacturonan is a source of Ca(2+) necessary for pollen germination. Although pollen-specific PMEs are traditionally associated with pollen tube elongation, this study provides strong evidence that PME48 impacts the mechanical properties of the intine wall during maturation of the pollen grain, which, in turn, influences pollen grain germination. PMID:25524442

  16. Investigating atmospheric transport of Ambrosia pollen from the Pannonian Plain towards the Balkan region with DEHM-Pollen

    NASA Astrophysics Data System (ADS)

    Ambelas Skjoth, C.

    2009-04-01

    The pollen grains of Ambrosia spp. are considered to be important aeroallergens. The threshold value for clinical symptoms for ragweed pollen grains for the majority of sensitised patients is below 20 grains/m3. Ambrosia pollen appears to induce asthma about twice as often as other pollen. Each ragweed plant produces millions of pollen grains that are small (18-22 μm) and suitable for long-range transport when conditions are favourable. In this study we use DEHM-Pollen to investigate if the Pannonian Plain could be the source area for observed episodes of Ambrosia pollen in the Balkans. A possible Ambrosia pollen inventory for various regions in the Pannonian Plain was constructed using detailed land cover data from Serbia, Hungary, Austria, Croatia, Bosnia-Herzegovina, Slovakia, Romania and Czech Republic in combination with measurements of the annual load of Ambrosia pollen in the source areas. A simple unified pollen release model (SUPREME) was calibrated against daily measurements from Novi Sad in Serbia and implemented in DEHM-Pollen with the Ambrosia pollen inventory. Model simulations was then performed with DEHM-Pollen for the months August and September 2007 and compared with measurements from stations outside the Pannonian Plain. The simulations several times indicate regional scale transport from the Pannonian Plain towards the Balkan region including the 26th - 27th of August and the 1st and 2nd September. During these episode air masses passed over parts of Bosnia and Herzegovina, Southern Serbia, Albania and Macedonia. The verifying measurements at Skopje (Macedonia) show episodes of elevated Ambrosia pollen concentration the 26th -27th of August and for the 1st and 2nd of September. The model experiments with DEHM-Pollen strongly indicate that the Pannonian Plain alone can be a source to significant Ambrosia pollen concentrations in the Balkans. The methods and the model results look promising with respect to future numerical forecasting of Ambrosia

  17. Honey loading for pollen collection: regulation of crop content in honeybee pollen foragers on leaving hive

    NASA Astrophysics Data System (ADS)

    Harano, Ken-ichi; Mitsuhata-Asai, Akiko; Sasaki, Masami

    2014-07-01

    Before foraging honeybees leave the hive, each bee loads its crop with some amount of honey "fuel" depending on the distance to the food source and foraging experience. For pollen collection, there is evidence that foragers carry additional honey as "glue" to build pollen loads. This study examines whether pollen foragers of the European honeybee Apis mellifera regulate the size of the crop load according to food-source distances upon leaving the hive and how foraging experience affects load regulation. The crop contents of bees foraging on crape myrtle Lagerstroemia indica, which has no nectary, were larger than those foraging on nectar from other sources, confirming a previous finding that pollen foragers carry glue in addition to fuel honey from the hive. Crop contents of both waggle dancers and dance followers showed a significant positive correlation with waggle-run durations. These results suggest that bees carry a distance-dependent amount of fuel honey in addition to a fixed amount of glue honey. Crop contents on leaving the hive were statistically larger in dancers than followers. Based on these results, we suggest that pollen foragers use information obtained through foraging experience to adjust crop contents on leaving the hive.

  18. PHARUS airborne SAR concept

    NASA Astrophysics Data System (ADS)

    Snoeij, Paul; Pouwels, Henk; Koomen, Peter J.; Hoogeboom, Peter

    1995-11-01

    PHARUS (phased array universal SAR) is an airborne SAR concept which is being developed in the Netherlands. The PHARUS system differs from other airborne SARs by the use of a phased array antenna, which provides both for the flexibility in the design as well as for a compact, light-weight instrument that can be carried on small aircraft. The concept allows for the construction of airborne SAR systems on a common generic basis but tailored to specific user needs and can be seen as a preparation for future spaceborne SAR systems using solid state transmitters with electronically steerable phased array antenna. The whole approach is aimed at providing an economic and yet technically sophisticated solution to remote sensing or surveying needs of a specific user. The solid state phased array antenna consists of a collection of radiating patches; the design flexibility for a large part resides in the freedom to choose the number of patches, and thereby the essential radar performance parameters such as resolution and swath width. Another consequence of the use of the phased array antenna is the system's compactness and the possibility to rigidly mount it on a small aircraft. The use of small aircraft of course considerably improves the cost/benefit ratio of the use of airborne SAR. Flight altitude of the system is flexible between about 7,000 and 40,000 feet, giving much operational freedom within the meteo and airspace control limits. In the PHARUS concept the airborne segment is complemented by a ground segment, which consists of a SAR processor, possibly extended by a matching image processing package. (A quick look image is available in real-time on board the aircraft.) The SAR processor is UNIX based and runs on easily available hardware (SUN station). Although the additional image processing software is available, the SAR processing software is nevertheless designed to be able to interface with commercially available image processing software, as well as being able

  19. Accounting for What Counts

    ERIC Educational Resources Information Center

    Milner, Joseph O.; Ferran, Joan E.; Martin, Katharine Y.

    2003-01-01

    No Child Left Behind legislation makes it clear that outside evaluators determine what gets taught in the classroom. It is important to ensure they measure what truly counts in school. This fact is poignantly and sadly true for the under funded, poorly resourced, "low performing" schools that may be hammered by administration accountants in the…

  20. Making Research Count

    ERIC Educational Resources Information Center

    Appleby, Yvon; Kerwin, Marie; McCulloch, Sue

    2008-01-01

    Making research count in the education sector is often difficult to achieve as people, quite properly, question its relevance, purpose and impact. One of the significant barriers to research supporting practice in the lifelong learning sector is that funded research carried out in higher education institutions is frequently privileged above…

  1. LOW ENERGY COUNTING CHAMBERS

    DOEpatents

    Hayes, P.M.

    1960-02-16

    A beta particle counter adapted to use an end window made of polyethylene terephthalate was designed. The extreme thinness of the film results in a correspondingly high transmission of incident low-energy beta particles by the window. As a consequence, the counting efficiency of the present counter is over 40% greater than counters using conventional mica end windows.

  2. Counting digital filters

    NASA Technical Reports Server (NTRS)

    Zohar, S. (Inventor)

    1973-01-01

    Several embodiments of a counting digital filter of the non-recursive type are disclosed. In each embodiment two registers, at least one of which is a shift register, are included. The shift register received j sub x-bit data input words bit by bit. The kth data word is represented by the integer.

  3. What Counts as Evidence?

    ERIC Educational Resources Information Center

    Dougherty Stahl, Katherine A.

    2014-01-01

    Each disciplinary community has its own criteria for determining what counts as evidence of knowledge in their academic field. The criteria influence the ways that a community's knowledge is created, communicated, and evaluated. Situating reading, writing, and language instruction within the content areas enables teachers to explicitly…

  4. Airborne radioactive contamination monitoring

    SciTech Connect

    Whitley, C.R.; Adams, J.R.; Bounds, J.A.; MacArthur, D.W.

    1996-03-01

    Current technologies for the detection of airborne radioactive contamination do not provide real-time capability. Most of these techniques are based on the capture of particulate matter in air onto filters which are then processed in the laboratory; thus, the turnaround time for detection of contamination can be many days. To address this shortcoming, an effort is underway to adapt LRAD (Long-Range-Alpha-Detection) technology for real-time monitoring of airborne releases of alpa-emitting radionuclides. Alpha decays in air create ionization that can be subsequently collected on electrodes, producing a current that is proportional to the amount of radioactive material present. Using external fans on a pipe containing LRAD detectors, controlled samples of ambient air can be continuously tested for the presence of radioactive contamination. Current prototypes include a two-chamber model. Sampled air is drawn through a particulate filter and then through the first chamber, which uses an electrostatic filter at its entrance to remove ambient ionization. At its exit, ionization that occurred due to the presence of radon is collected and recorded. The air then passes through a length of pipe to allow some decay of short-lived radon species. A second chamber identical to the first monitors the remaining activity. Further development is necessary on air samples without the use of particulate filtering, both to distinguish ionization that can pass through the initial electrostatic filter on otherwise inert particulate matter from that produced through the decay of radioactive material and to separate both of these from the radon contribution. The end product could provide a sensitive, cost-effective, real-time method of determining the presence of airborne radioactive contamination.

  5. Quantitation of Japanese cedar pollen and radiocesium adhered to nonwoven fabric masks worn by the general population.

    PubMed

    Higaki, Shogo; Shirai, Hideharu; Hirota, Masahiro; Takeda, Eisuke; Yano, Yukiko; Shibata, Akira; Mishima, Yoshitaka; Yamamoto, Hiromi; Miyazawa, Kiyoshi

    2014-08-01

    In the spring of 2012, a year after the Fukushima Daiichi nuclear disaster, radiocesium-contaminated Japanese cedar pollen may have caused internal exposure to the general population by inhalation. To determine if pollen had been contaminated through uptake of radiocesium by Japanese cedars and was therefore contributing to inhalation doses, the authors measured radiocesium and Japanese cedar pollen adhered to masks worn by 68 human subjects residing in eastern Japan, including Fukushima prefecture, for 8 wk in the spring of 2012. The maximum cumulative Cs and Cs radioactivities on masks worn by an individual were 21 ± 0.36 Bq and 15 ± 0.22 Bq, respectively, and the estimated effective dose during the 8 wk was 0.494 μSv. The average estimated effective dose during the 8 wk was 0.149 μSv in Fukushima prefecture and 0.015 μSv in other prefectures, including Tokyo metropolitan. The correlation between radiocesium activity and the Japanese cedar pollen count was moderate. However, imaging-plate and light microscopy observations showed that the main source of radiocesium adhered to masks was fugitive dust. PMID:24978283

  6. Occupational Allergy to Peach (Prunus persica) Tree Pollen and Potential Cross-Reactivity between Rosaceae Family Pollens.

    PubMed

    Jiang, Nannan; Yin, Jia; Mak, Philip; Wen, Liping

    2015-10-01

    Orchard workers in north China are highly exposed to orchard pollens, especially peach and other Rosaceae family pollens during pollination season. The aim of this study was to investigate whether occupational allergy to peach tree pollen as a member of Rosaceae family is IgE-mediated and to evaluate the cross-reactivity among Rosaceae family pollens. Allergen skin test and conjunctival challenge test were performed; enzyme linked immune-sorbent assay (ELISA), inhibiting ELISA, western immunoblotting and inhibiting western immunoblotting were done with Rosaceae family orchard pollens, including peach, apricot, cherry, apple and pear tree pollens. Mass spectrometry was also performed to probe the main allergen component and cross-reactive protein. Sensitizations to peach pollen were found in both skin test and conjunctival challenge in the patients. Serum specific IgE to three pollens (peach, apricot and cherry) were detected through ELISA. When peach pollen used as solid phase, ELISA inhibition revealed other four kinds of pollens capable of inducing partial to strong inhibitions (45% to 87%), with the strongest inhibition belonging to apricot pollen (87%). Western blotting showed predominant IgE binding to a 20 KD protein among these pollens, which appeared to be a cross-reactive allergen component through western blotting inhibition. It was recognized as a protein homologous to glutathione s-transferase 16 from Arabidopsis thaliana. Peach and other Rosaceae family tree pollen may serve as a potential cause of IgE mediated occupational respiratory disease in orchard workers in north China. PMID:26742437

  7. Airborne Raman lidar

    NASA Astrophysics Data System (ADS)

    Heaps, Wm. S.; Burris, J.

    1996-12-01

    We designed and tested an airborne lidar system using Raman scattering to make simultaneous measurements of methane, water vapor, and temperature in a series of flights on a NASA-operated C-130 aircraft. We present the results for methane detection, which show that the instrument has the requisite sensitivity to atmospheric trace gases. Ultimately these measurements can be used to examine the transport of chemically processed air from within the polar vortex to mid-latitudinal regions and the exchange of stratospheric air between tropical and mid-latitudinal regions.

  8. Airborne Oceanographic Lidar System

    NASA Technical Reports Server (NTRS)

    Bressel, C.; Itzkan, I.; Nunes, J. E.; Hoge, F.

    1977-01-01

    The Airborne Oceanographic Lidar (AOL), a spatially scanning range-gated device installed on board a NASA C-54 aircraft, is described. The AOL system is capable of measuring topographical relief or water depth (bathymetry) with a range resolution of plus or minus 0.3 m in the vertical dimension. The system may also be used to measure fluorescent spectral signatures from 3500 to 8000 A with a resolution of 100 A. Potential applications of the AOL, including sea state measurements, water transparency assessments, oil spill identification, effluent identification and crop cover assessment are also mentioned.

  9. Pollensomes as Natural Vehicles for Pollen Allergens.

    PubMed

    Prado, Noela; De Linares, Concepción; Sanz, María L; Gamboa, Pedro; Villalba, Mayte; Rodríguez, Rosalía; Batanero, Eva

    2015-07-15

    Olive (Olea europaea) pollen constitutes one of the most important allergen sources in the Mediterranean countries and some areas of the United States, South Africa, and Australia. Recently, we provided evidence that olive pollen releases nanovesicles of respirable size, named generically pollensomes, during in vitro germination. Olive pollensomes contain allergens, such as Ole e 1, Ole e 11, and Ole e 12, suggesting a possible role in allergy. The aim of this study was to assess the contribution of pollensomes to the allergic reaction. We show that pollensomes exhibit allergenic activity in terms of patients' IgE-binding capacity, human basophil activation, and positive skin reaction in sensitized patients. Furthermore, allergen-containing pollensomes have been isolated from three clinically relevant nonphylogenetically related species: birch (Betula verrucosa), pine (Pinus sylvestris), and ryegrass (Lolium perenne). Most interesting, pollensomes were isolated from aerobiological samples collected with an eight-stage cascade impactor collector, indicating that pollensomes secretion is a naturally occurring phenomenon. Our findings indicate that pollensomes may represent widespread vehicles for pollen allergens, with potential implications in the allergic reaction. PMID:26041541

  10. Cell-Cell Interactions during pollen tube guidance

    SciTech Connect

    Daphne Preuss

    2009-03-31

    The long-term goal of this research is to identify the signaling molecules that mediate plant cell-cell interactions during pollination. The immediate goals of this project are to perform genetic and molecular analysis of pollen tube guidance. Specifically, we proposed to: 1. Characterize the pistil components that direct pollen tube navigation using the Arabidopsis thaliana in vitro pollen tube guidance system 2. Identify pistil signals that direct pollen tube guidance by a) using microarrays to profile gene expression in developing pistils, and b) employing proteomics and metabolomics to isolate pollen tube guidance signals. 3. Explore the genetic basis of natural variation in guidance signals, comparing the in vitro interactions between pollen and pistils from A. thaliana and its close relatives.

  11. Pollen DNA barcoding: current applications and future prospects.

    PubMed

    Bell, Karen L; de Vere, Natasha; Keller, Alexander; Richardson, Rodney T; Gous, Annemarie; Burgess, Kevin S; Brosi, Berry J

    2016-09-01

    Identification of the species origin of pollen has many applications, including assessment of plant-pollinator networks, reconstruction of ancient plant communities, product authentication, allergen monitoring, and forensics. Such applications, however, have previously been limited by microscopy-based identification of pollen, which is slow, has low taxonomic resolution, and has few expert practitioners. One alternative is pollen DNA barcoding, which could overcome these issues. Recent studies demonstrate that both chloroplast and nuclear barcoding markers can be amplified from pollen. These recent validations of pollen metabarcoding indicate that now is the time for researchers in various fields to consider applying these methods to their research programs. In this paper, we review the nascent field of pollen DNA barcoding and discuss potential new applications of this technology, highlighting existing limitations and future research developments that will improve its utility in a wide range of applications. PMID:27322652

  12. Airborne concentrations of peanut protein.

    PubMed

    Johnson, Rodney M; Barnes, Charles S

    2013-01-01

    Food allergy to peanut is a significant health problem, and there are reported allergic reactions to peanuts despite not eating or having physical contact with peanuts. It is presumed that an allergic reaction may have occurred from inhalation of airborne peanut allergens. The purpose of this study was to detect the possible concentrations of airborne peanut proteins for various preparations and during specific activities. Separate Ara h 1 and Ara h 2 monoclonal enzyme-linked immunosorbent assays and a polyclonal sandwich enzyme immunoassay for peanuts were used to detect the amount of airborne peanut protein collected using a Spincon Omni 3000 air collector (Sceptor Industries, Inc., Kansas City, MO) under different peanut preparation methods and situations. Air samples were measured for multiple peanut preparations and scenarios. Detectable amounts of airborne peanut protein were measured using a whole peanut immunoassay when removing the shells of roasted peanut. No airborne peanut allergen (Ara h 1 or Ara h 2) or whole peanut protein above the LLD was measured in any of the other peanut preparation collections. Ara h 1, Ara h 2, and polyclonal peanut proteins were detected from water used to boil peanuts. Small amounts of airborne peanut protein were detected in the scenario of removing shells from roasted peanuts; however, Ara h 1 and Ara h 2 proteins were unable to be consistently detected. Although airborne peanut proteins were detected, the concentration of airborne peanut protein that is necessary to elicit a clinical allergic reaction is unknown. PMID:23406937

  13. Airborne ballistic camera tracking systems

    NASA Technical Reports Server (NTRS)

    Redish, W. L.

    1976-01-01

    An operational airborne ballistic camera tracking system was tested for operational and data reduction feasibility. The acquisition and data processing requirements of the system are discussed. Suggestions for future improvements are also noted. A description of the data reduction mathematics is outlined. Results from a successful reentry test mission are tabulated. The test mission indicated that airborne ballistic camera tracking systems are feasible.

  14. Pollen Feeding and Reproductive Biology of Heliconius Butterflies

    PubMed Central

    Gilbert, Lawrence E.

    1972-01-01

    Butterflies of the neotropical Genus Heliconius feed on pollen. This is the first known instance in butterflies of a habit that is well known for other insects. The butterflies remove amino acids and proteins from pollen; this feeding innovation plays a role in the reproductive and population biology of these insects. It is suggested that other animals may use pollen in a similar fashion. Images PMID:16591992

  15. Palynological mapping of vertical microseepage over Dragoon and Pollen fields

    SciTech Connect

    Groth, P.K.H.

    1986-05-01

    Fossil pollen was recovered from multiple shallow boreholes in Colorado's D-J basin. Detailed light transmissibility of a particular pollen species was obtained, and the data were contoured. Extensions to Dragoon and Byers fields were mapped, and new fields Pollen and Quill were mapped and predicted. The patented technique recognizes mineral oxidation surrounding reduced or reduction-maintained areas of microseepage above reservoirs that are present at a depth of 7500 ft.

  16. Links between pollen, atopy and the asthma epidemic.

    PubMed

    Taylor, Philip E; Jacobson, Kraig W; House, James M; Glovsky, M Michael

    2007-01-01

    Pollen allergy has been found in 80-90% of childhood asthmatics and 40-50% of adult-onset asthmatics. Despite the high prevalence of atopy in asthmatics, a causal relationship between the allergic response and asthma has not been clearly established. Pollen grains are too large to penetrate the small airways where asthma occurs. Yet pollen cytoplasmic fragments are respirable and are likely correlated with the asthmatic response in allergic asthmatics. In this review, we outline the mechanism of pollen fragmentation and possible pathophysiology of pollen fragment-induced asthma. Pollen grains rupture within the male flowers and emit cytoplasmic debris when winds or other disturbances disperse the pollen. Peak levels of grass and birch pollen allergens in the atmosphere correlated with the occurrence of moist weather conditions during the flowering period. Thunderstorm asthma epidemics may be triggered by grass pollen rupture in the atmosphere and the entrainment of respirable-sized particles in the outflows of air masses at ground level. Pollen contains nicotinamide adenine dinucleotide phosphate (reduced) oxidases and bioactive lipid mediators which likely contribute to the inflammatory response. Several studies have examined synergistic effects and enhanced immune response from interaction in the atmosphere, or from co-deposition in the airways, of pollen allergens, endogenous pro-inflammatory agents, and the particulate and gaseous fraction of combustion products. Pollen and fungal fragments also contain compounds that can suppress reactive oxidants and quench free radicals. It is important to know more about how these substances interact to potentially enhance, or even ameliorate, allergic asthma. PMID:17536216

  17. Dynamics of sulfate and nitrate dry deposition associated with pollen

    SciTech Connect

    Khalili, E.K.

    1988-01-01

    A field study of pollen dispersion and deposition from a remote forested area in Northern Wisconsin has been undertaken. Although the experiments constitute a case study in Wisconsin, the experimental site was chosen, which represents much of the Eastern US and Europe where acid rain is considered an important environmental problem. Measurements of dry deposition of pollen were made during the pollination season (May and June, 1987). Deposited particles were weighted to determine mass fluxes, then washed and subjected to ion chromatographic analysis for sulfate and nitrate. Ambient concentration of pollen were measured by a coarse particle sampler (Noll Inertial Rotary Impact) during the same time period. The chemical analysis of pollen species collected around the sampling site as well as commercially available pollen demonstrated that sulfate and nitrate were present on all pollen samples. Many trace metals such as Fe, Al, Zn, Cu, Mn, Cd, Pb, Ca, and Si and organic acids were quantified. It was hypothesized that pollen accumulate extraneous amounts of non-essential, as well as essential elements from the soil supplying nutrient. Therefore, pollen can be used as a fingerprint for the availability and the level of contamination of a particular element in the forest soil environment. A model developed for measurement of coarse particle dry deposition was utilized to measure the pollen dry deposition velocity. It was shown that depositional velocity of pollen exceeds the settling velocity by a factor of 3 to 4, and both V{sub d} and fluxes of pollen grains increase with wind speed. Finally, the role of pollen dispersion and deposition has been discussed and emphasized for modeling of lake acidification in forested region.

  18. A laboratory assessment of the Waveband Integrated Bioaerosol Sensor (WIBS-4) using individual samples of pollen and fungal spore material

    NASA Astrophysics Data System (ADS)

    Healy, David A.; O'Connor, David J.; Burke, Aoife M.; Sodeau, John R.

    2012-12-01

    A Bioaerosol sensing instrument referred to as WIBS-4, designed to continuously monitor ambient bioaerosols on-line, has been used to record a multiparameter “signature” from each of a number of Primary Biological Aerosol Particulate (PBAP) samples found in air. These signatures were obtained in a controlled laboratory environment and are based on the size, asymmetry (“shape”) and auto-fluorescence of the particles. Fifteen samples from two separate taxonomic ranks (kingdoms), Plantae (×8) and Fungi (×7) were individually introduced to the WIBS-4 for measurement along with two non-fluorescing chemical solids, common salt and chalk. Over 2000 individual-particle measurements were recorded for each sample type and the ability of the WIBS spectroscopic technique to distinguish between chemicals, pollen and fungal spore material was examined by identifying individual PBAP signatures. The results obtained show that WIBS-4 could potentially be a very useful analytical tool for distinguishing between natural airborne PBAP samples, such as the fungal spores and may potentially play an important role in detecting and discriminating the toxic fungal spore, Aspergillus fumigatus, from others in real-time. If the sizing range of the commercial instrument was customarily increased and permitted to operate simultaneously in its two sizing ranges, pollen and spores could potentially be discriminated between. The data also suggest that the gain setting sensitivity on the detector would also have to be reduced by a factor >5, to routinely detect, in-range fluorescence measurements for pollen samples.

  19. Airborne transmission of lyssaviruses.

    PubMed

    Johnson, N; Phillpotts, R; Fooks, A R

    2006-06-01

    In 2002, a Scottish bat conservationist developed a rabies-like disease and subsequently died. This was caused by infection with European bat lyssavirus 2 (EBLV-2), a virus closely related to Rabies virus (RABV). The source of this infection and the means of transmission have not yet been confirmed. In this study, the hypothesis that lyssaviruses, particularly RABV and the bat variant EBLV-2, might be transmitted via the airborne route was tested. Mice were challenged via direct introduction of lyssavirus into the nasal passages. Two hours after intranasal challenge with a mouse-adapted strain of RABV (Challenge Virus Standard), viral RNA was detectable in the tongue, lungs and stomach. All of the mice challenged by direct intranasal inoculation developed disease signs by 7 days post-infection. Two out of five mice challenged by direct intranasal inoculation of EBLV-2 developed disease between 16 and 19 days post-infection. In addition, a simple apparatus was evaluated in which mice could be exposed experimentally to infectious doses of lyssavirus from an aerosol. Using this approach, mice challenged with RABV, but not those challenged with EBLV-2, were highly susceptible to infection by inhalation. These data support the hypothesis that lyssaviruses, and RABV in particular, can be spread by airborne transmission in a dose-dependent manner. This could present a particular hazard to personnel exposed to aerosols of infectious RABV following accidental release in a laboratory environment. PMID:16687600

  20. Inflated Sporopollenin Exine Capsules Obtained from Thin-Walled Pollen

    PubMed Central

    Park, Jae Hyeon; Seo, Jeongeun; Jackman, Joshua A.; Cho, Nam-Joon

    2016-01-01

    Sporopollenin is a physically robust and chemically resilient biopolymer that comprises the outermost layer of pollen walls and is the first line of defense against harsh environmental conditions. The unique physicochemical properties of sporopollenin increasingly motivate the extraction of sporopollenin exine capsules (SECs) from pollen walls as a renewable source of organic microcapsules for encapsulation applications. Despite the wide range of different pollen species with varying sizes and wall thicknesses, faithful extraction of pollen-mimetic SECs has been limited to thick-walled pollen capsules with rigid mechanical properties. There is an unmet need to develop methods for producing SECs from thin-walled pollen capsules which constitute a large fraction of all pollen species and have attractive materials properties such as greater aerosol dispersion. Herein, we report the first successful extraction of inflated SEC microcapsules from a thin-walled pollen species (Zea mays), thereby overcoming traditional challenges with mechanical stability and loss of microstructure. Morphological and compositional characterization of the SECs obtained by the newly developed extraction protocol confirms successful protein removal along with preservation of nanoscale architectural features. Looking forward, there is excellent potential to apply similar strategies across a wide range of unexplored thin-walled pollen species. PMID:27302853

  1. A DNA barcoding approach to characterize pollen collected by honeybees.

    PubMed

    Galimberti, Andrea; De Mattia, Fabrizio; Bruni, Ilaria; Scaccabarozzi, Daniela; Sandionigi, Anna; Barbuto, Michela; Casiraghi, Maurizio; Labra, Massimo

    2014-01-01

    In the present study, we investigated DNA barcoding effectiveness to characterize honeybee pollen pellets, a food supplement largely used for human nutrition due to its therapeutic properties. We collected pollen pellets using modified beehives placed in three zones within an alpine protected area (Grigna Settentrionale Regional Park, Italy). A DNA barcoding reference database, including rbcL and trnH-psbA sequences from 693 plant species (104 sequenced in this study) was assembled. The database was used to identify pollen collected from the hives. Fifty-two plant species were identified at the molecular level. Results suggested rbcL alone could not distinguish among congeneric plants; however, psbA-trnH identified most of the pollen samples at the species level. Substantial variability in pollen composition was observed between the highest elevation locality (Alpe Moconodeno), characterized by arid grasslands and a rocky substrate, and the other two sites (Cornisella and Ortanella) at lower altitudes. Pollen from Ortanella and Cornisella showed the presence of typical deciduous forest species; however in samples collected at Ortanella, pollen of the invasive Lonicera japonica, and the ornamental Pelargonium x hortorum were observed. Our results indicated pollen composition was largely influenced by floristic local biodiversity, plant phenology, and the presence of alien flowering species. Therefore, pollen molecular characterization based on DNA barcoding might serve useful to beekeepers in obtaining honeybee products with specific nutritional or therapeutic characteristics desired by food market demands. PMID:25296114

  2. Inflated Sporopollenin Exine Capsules Obtained from Thin-Walled Pollen.

    PubMed

    Park, Jae Hyeon; Seo, Jeongeun; Jackman, Joshua A; Cho, Nam-Joon

    2016-01-01

    Sporopollenin is a physically robust and chemically resilient biopolymer that comprises the outermost layer of pollen walls and is the first line of defense against harsh environmental conditions. The unique physicochemical properties of sporopollenin increasingly motivate the extraction of sporopollenin exine capsules (SECs) from pollen walls as a renewable source of organic microcapsules for encapsulation applications. Despite the wide range of different pollen species with varying sizes and wall thicknesses, faithful extraction of pollen-mimetic SECs has been limited to thick-walled pollen capsules with rigid mechanical properties. There is an unmet need to develop methods for producing SECs from thin-walled pollen capsules which constitute a large fraction of all pollen species and have attractive materials properties such as greater aerosol dispersion. Herein, we report the first successful extraction of inflated SEC microcapsules from a thin-walled pollen species (Zea mays), thereby overcoming traditional challenges with mechanical stability and loss of microstructure. Morphological and compositional characterization of the SECs obtained by the newly developed extraction protocol confirms successful protein removal along with preservation of nanoscale architectural features. Looking forward, there is excellent potential to apply similar strategies across a wide range of unexplored thin-walled pollen species. PMID:27302853

  3. Pollen Tube Growth and Self-Compatibility in Almond

    PubMed Central

    Socias i Company, Rafel; Kodad, Ossama; Fernández i Martí, Àngel; Alonso, José M.

    2013-01-01

    Although pollen tube growth has been an important criterion for self-compatibility evaluation in almond, there is not a clear-cut separation between positive and negative growth of pollen tubes in the different genotypes. The examination of pollen tube growth after selfing almond seedlings has allowed establishing different levels of compatibility, but not a clear-cut separation between self-compatible (SC) and self-incompatible (SI) genotypes, related to the presence of pseudo-self-compatibility in almond. Consequently, a relationship between pollen tube growth and self-compatibility in almond may be established for evaluating the seedlings in breeding programs. PMID:27137365

  4. A DNA Barcoding Approach to Characterize Pollen Collected by Honeybees

    PubMed Central

    Bruni, Ilaria; Scaccabarozzi, Daniela; Sandionigi, Anna; Barbuto, Michela; Casiraghi, Maurizio; Labra, Massimo

    2014-01-01

    In the present study, we investigated DNA barcoding effectiveness to characterize honeybee pollen pellets, a food supplement largely used for human nutrition due to its therapeutic properties. We collected pollen pellets using modified beehives placed in three zones within an alpine protected area (Grigna Settentrionale Regional Park, Italy). A DNA barcoding reference database, including rbcL and trnH-psbA sequences from 693 plant species (104 sequenced in this study) was assembled. The database was used to identify pollen collected from the hives. Fifty-two plant species were identified at the molecular level. Results suggested rbcL alone could not distinguish among congeneric plants; however, psbA-trnH identified most of the pollen samples at the species level. Substantial variability in pollen composition was observed between the highest elevation locality (Alpe Moconodeno), characterized by arid grasslands and a rocky substrate, and the other two sites (Cornisella and Ortanella) at lower altitudes. Pollen from Ortanella and Cornisella showed the presence of typical deciduous forest species; however in samples collected at Ortanella, pollen of the invasive Lonicera japonica, and the ornamental Pelargonium x hortorum were observed. Our results indicated pollen composition was largely influenced by floristic local biodiversity, plant phenology, and the presence of alien flowering species. Therefore, pollen molecular characterization based on DNA barcoding might serve useful to beekeepers in obtaining honeybee products with specific nutritional or therapeutic characteristics desired by food market demands. PMID:25296114

  5. Foldable structures and the natural design of pollen grains

    PubMed Central

    Katifori, Eleni; Alben, Silas; Cerda, Enrique; Nelson, David R.; Dumais, Jacques

    2010-01-01

    Upon release from the anther, pollen grains of angiosperm flowers are exposed to a dry environment and dehydrate. To survive this process, pollen grains possess a variety of physiological and structural adaptations. Perhaps the most striking of these adaptations is the ability of the pollen wall to fold onto itself to prevent further desiccation. Roger P. Wodehouse coined the term harmomegathy for this folding process in recognition of the critical role it plays in the survival of the pollen grain. There is still, however, no quantitative theory that explains how the structure of the pollen wall contributes to harmomegathy. Here we demonstrate that simple geometrical and mechanical principles explain how wall structure guides pollen grains toward distinct folding pathways. We found that the presence of axially elongated apertures of high compliance is critical for achieving a predictable and reversible folding pattern. Moreover, the intricate sculpturing of the wall assists pollen closure by preventing mirror buckling of the surface. These results constitute quantitative structure-function relationships for pollen harmomegathy and provide a framework to elucidate the functional significance of the very diverse pollen morphologies observed in angiosperms. PMID:20404200

  6. Inflated Sporopollenin Exine Capsules Obtained from Thin-Walled Pollen

    NASA Astrophysics Data System (ADS)

    Park, Jae Hyeon; Seo, Jeongeun; Jackman, Joshua A.; Cho, Nam-Joon

    2016-06-01

    Sporopollenin is a physically robust and chemically resilient biopolymer that comprises the outermost layer of pollen walls and is the first line of defense against harsh environmental conditions. The unique physicochemical properties of sporopollenin increasingly motivate the extraction of sporopollenin exine capsules (SECs) from pollen walls as a renewable source of organic microcapsules for encapsulation applications. Despite the wide range of different pollen species with varying sizes and wall thicknesses, faithful extraction of pollen-mimetic SECs has been limited to thick-walled pollen capsules with rigid mechanical properties. There is an unmet need to develop methods for producing SECs from thin-walled pollen capsules which constitute a large fraction of all pollen species and have attractive materials properties such as greater aerosol dispersion. Herein, we report the first successful extraction of inflated SEC microcapsules from a thin-walled pollen species (Zea mays), thereby overcoming traditional challenges with mechanical stability and loss of microstructure. Morphological and compositional characterization of the SECs obtained by the newly developed extraction protocol confirms successful protein removal along with preservation of nanoscale architectural features. Looking forward, there is excellent potential to apply similar strategies across a wide range of unexplored thin-walled pollen species.

  7. The Effects of Pollen-Enriched Pollen Substitute on Winter Cluster Size and the Prevalence of Nosema ceranae in Russian Honey Bee Colonies

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study determined the effects of feeding a pollen substitute enriched with pollen and feeding protein in plastic frames placed directly in the brood nest on the growth of Russian honey bee colonies through the winter. Colonies were fed: 1) a mixture of 1/2 pollen and 1/2 commercial pollen substi...

  8. The Pollen Receptor Kinase LePRK2 Mediates Growth-Promoting Signals and Positively Regulates Pollen Germination and Tube Growth

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A pollen receptor kinase from tomato, LePRK2, has been implicated in signaling during pollen germination and tube growth as well as in mediating pollen (tube)-pistil communication. Here w...

  9. Counting every quantum

    PubMed Central

    Sakitt, B.

    1972-01-01

    1. Human subjects were asked to rate both blanks and very dim flashes of light under conditions of complete dark adaptation at 7° in the periphery. The ratings used were 0, 1, 2, 3, 4, 5, and 6. 2. For one subject (B.S.) the distributions of ratings were approximately Poisson distributions. The data were consistent with each rating being the actual number of effective quantal absorptions plus the number of noise events. This subject was presumably able to count every rod signal (effective absorptions plus noise). 3. For two other subjects, the data were consistent with the ratings being one less (L.F.) and two less (K.D.) than the number of effective absorptions plus noise. They were able to count every rod signal beginning with 2 and 3 respectively. A fourth subject's erratic data could not be fitted. 4. The fraction of quanta incident at the cornea that resulted in a rod signal was estimated to be about 0·03 which is consistent with physical estimates of effective absorption for that retinal region. 5. A simulated forced choice experiment leads to an absolute threshold about 0·40 log units below the normal yes-no absolute threshold. This and other results indicate that subjects can use the sensory information they receive even when only 1, 2 or 3 quanta are effectively absorbed, depending on the individual. Humans may be able to count every action potential or every discrete burst of action potentials in some critical neurone. PMID:5046137

  10. Quantifying pollen-vegetation relationships to reconstruct forests using 19th-century forest composition and pollen data

    USGS Publications Warehouse

    Dawson, Andria; Paciorek, Christopher J.; McLachlan, Jason S.; Goring, Simon; Williams, John W.; Jackson, Stephen T.

    2016-01-01

    Mitigation of climate change and adaptation to its effects relies partly on how effectively land-atmosphere interactions can be quantified. Quantifying composition of past forest ecosystems can help understand processes governing forest dynamics in a changing world. Fossil pollen data provide information about past forest composition, but rigorous interpretation requires development of pollen-vegetation models (PVMs) that account for interspecific differences in pollen production and dispersal. Widespread and intensified land-use over the 19th and 20th centuries may have altered pollen-vegetation relationships. Here we use STEPPS, a Bayesian hierarchical spatial PVM, to estimate key process parameters and associated uncertainties in the pollen-vegetation relationship. We apply alternate dispersal kernels, and calibrate STEPPS using a newly developed Euro-American settlement-era calibration data set constructed from Public Land Survey data and fossil pollen samples matched to the settlement-era using expert elicitation. Models based on the inverse power-law dispersal kernel outperformed those based on the Gaussian dispersal kernel, indicating that pollen dispersal kernels are fat tailed. Pine and birch have the highest pollen productivities. Pollen productivity and dispersal estimates are generally consistent with previous understanding from modern data sets, although source area estimates are larger. Tests of model predictions demonstrate the ability of STEPPS to predict regional compositional patterns.

  11. The pollen enigma: modulation of the allergic immune response by non-allergenic, pollen-derived compounds.

    PubMed

    Gilles, Stefanie; Behrendt, Heidrun; Ring, Johannes; Traidl-Hoffmann, Claudia

    2012-01-01

    The question what makes an allergen an allergen puzzled generations of researchers. Pollen grains of anemophilous plants are the most important allergen carriers in ambient air, and pollinosis is a highly prevalent multi-organ disease in civilized countries. In the past, research on the allergenicity of pollen has mainly focused on elucidating genetic predisposing factors and on defining certain structural characteristics of pollen derived allergens. Recently, studies extended to the analysis of non-allergenic, adjuvant mediators co-released from pollen. Besides active proteases and oxidases, extracts of pollen contain low molecular weight molecules like pollen-associated lipid mediators or adenosine exhibiting a potential to stimulate and modulate cultured human immune cells. This article reviews our current knowledge on non-allergenic, protein and non-protein compounds from pollen and their in vitro and in vivo effects on the allergic immune response. To ultimately judge the physiological relevance of these compounds, a systematic approach will be needed comparing their releasability, content and activity in different, allergenic and non-allergenic, pollen species. System biology such as proteome and metabolome analysis will be a useful future approach to better understand pollen biology. PMID:22390694

  12. Quantifying pollen-vegetation relationships to reconstruct ancient forests using 19th-century forest composition and pollen data

    NASA Astrophysics Data System (ADS)

    Dawson, Andria; Paciorek, Christopher J.; McLachlan, Jason S.; Goring, Simon; Williams, John W.; Jackson, Stephen T.

    2016-04-01

    Mitigation of climate change and adaptation to its effects relies partly on how effectively land-atmosphere interactions can be quantified. Quantifying composition of past forest ecosystems can help understand processes governing forest dynamics in a changing world. Fossil pollen data provide information about past forest composition, but rigorous interpretation requires development of pollen-vegetation models (PVMs) that account for interspecific differences in pollen production and dispersal. Widespread and intensified land-use over the 19th and 20th centuries may have altered pollen-vegetation relationships. Here we use STEPPS, a Bayesian hierarchical spatial PVM, to estimate key process parameters and associated uncertainties in the pollen-vegetation relationship. We apply alternate dispersal kernels, and calibrate STEPPS using a newly developed Euro-American settlement-era calibration data set constructed from Public Land Survey data and fossil pollen samples matched to the settlement-era using expert elicitation. Models based on the inverse power-law dispersal kernel outperformed those based on the Gaussian dispersal kernel, indicating that pollen dispersal kernels are fat tailed. Pine and birch have the highest pollen productivities. Pollen productivity and dispersal estimates are generally consistent with previous understanding from modern data sets, although source area estimates are larger. Tests of model predictions demonstrate the ability of STEPPS to predict regional compositional patterns.

  13. Effects of particle size and velocity on burial depth of airborne particles in glass fiber filters

    SciTech Connect

    Higby, D.P.

    1984-11-01

    Air sampling for particulate radioactive material involves collecting airborne particles on a filter and then determining the amount of radioactivity collected per unit volume of air drawn through the filter. The amount of radioactivity collected is frequently determined by directly measuring the radiation emitted from the particles collected on the filter. Counting losses caused by the particle becoming buried in the filter matrix may cause concentrations of airborne particulate radioactive materials to be underestimated by as much as 50%. Furthermore, the dose calculation for inhaled radionuclides will also be affected. The present study was designed to evaluate the extent to which particle size and sampling velocity influence burial depth in glass-fiber filters. Aerosols of high-fired /sup 239/PuO/sub 2/ were collected at various sampling velocities on glass-fiber filters. The fraction of alp