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Sample records for aitken spa basin

  1. Detection and Extent of Ancient, Buried Mare Deposits in South Pole-Aitken Basin (SPA):Implications for Robotic Sampling

    NASA Astrophysics Data System (ADS)

    Petro, N. E.; Jolliff, B. L.; Gaddis, L. R.; Pieters, C. M.

    2010-12-01

    The origin of the large mafic anomaly associated with the interior of the South Pole-Aitken Basin has been inferred to be largely the result of iron-rich lower crustal/upper mantle material exposed at the surface and/or a combination of ancient mare basalts covered by younger crater/basin ejecta (cryptomare) interspersed with younger basalts [1-3]. However, the relative influence of either source is poorly constrained, due in part to the unknown abundance of cryptomare within SPA. Early geologic mapping of the interior of SPA identified several plains units, thought to represent basin ejecta deposits [4, 5]. Newer remotely sensed VIS-NIR wavelength data suggested the presence of more extensive deposits of ancient, buried basalts [2, 3, 6]. Mare basalts, when mantled by non-local, low-FeO material may appear to be non-mare plains units [7, 8]. Within SPA, because the regional basement material is inherently enriched in FeO, the mantling material imparts a dark, FeO-enriched, signature. In a survey of rock types within SPA, Pieters et al. [3] identified such a plains unit south of the Apollo Basin with a surface that is both dark and that contains an FeO-rich spectral signature. However, several small craters in the plains unit expose underlying basaltic materials or cryptomaria in this extensive (>75,000 km2), ancient (~3.89 Ga) unit [6, 9]. The positive identification and characterization of cryptomaria within SPA are facilitated by high-spatial and spectral resolution data from recent orbital missions (e.g., Kaguya, Chandrayaan-1, LRO). Hyperspectral data from the Moon Mineralogy Mapper and Multiband Imager for SPA show the presence of two primary mafic materials; a high-Ca pyroxene (gabbroic) signature is pervasive across the center of the basin and a noritic signature is present across the rest of SPA. High spatial resolution (10-0.5 m) images from the Kaguya Terrain Camera and LRO Narrow Angle Camera facilitate surface age dating and morphologic assessment of

  2. Sampling South Pole-Aitken Basin: The Moonrise Approach

    NASA Technical Reports Server (NTRS)

    Jolliff, B. L.; Shearer, C. K.; Cohen, B. A.

    2012-01-01

    The South Pole-Aitken basin (SPA) is the largest of the giant impact basins in the inner Solar System, and its location on Earth s Moon makes it the most accessible. Exploration of SPA through direct collection and analysis of representative materials addresses issues as fundamental as the characteristics of the chemical reservoir from which the Moon originated, early differentiation and production of crust and development of global asymmetry, relationships between magmatic activity and internal thermal evolution, and effects of giant impact events on the terrestrial planets. Owing to its great size and superposition relationships with other lunar impact basins, SPA is the oldest and as such anchors the lunar chronology. Moreover, numerous large impact craters and basins are contained within it such that materials (rocks) of the SPA basin contain a record of the early impact chronology, one less likely to have been affected by the large, late nearside basins (e.g., Imbrium). Understanding the early basin chronology is key to deciphering the sequence and effects of early giant impact bombardment of the inner Solar System. That record exists on the Moon, and materials of the SPA basin will allow us to read that record. Knowledge of the early bombardment history will test - and may reshape - a key paradigm relating to early Solar System evolution. Did the planets form with the alignment of today, or was there a major reorientation of the giant planets that led to destabilization of asteroid orbits, and a cataclysmic bombardment of the inner Solar System hundreds of millions of years after accretion of the planets? Implications include understanding environments for early life-supporting habitats on Earth and Mars, and relationships to new observations of extra-solar planetary systems.

  3. Surveying the South Pole-Aitken basin magnetic anomaly for remnant impactor metallic iron

    USGS Publications Warehouse

    Cahill, Joshua T.S.; Hagerty, Justin J.; Lawrence, David M.; Klima, Rachel L.; Blewett, David T.

    2014-01-01

    The Moon has areas of magnetized crust ("magnetic anomalies"), the origins of which are poorly constrained. A magnetic anomaly near the northern rim of South Pole-Aitken (SPA) basin was recently postulated to originate from remnant metallic iron emplaced by the SPA basin-forming impactor. Here, we remotely examine the regolith of this SPA magnetic anomaly with a combination of Clementine and Lunar Prospector derived iron maps for any evidence of enhanced metallic iron content. We find that these data sets do not definitively detect the hypothesized remnant metallic iron within the upper tens of centimeters of the lunar regolith.

  4. South Pole-Aitken Basin (SPA) Units Delineated by Measures of Surface Roughness: Implications for the History and Evolution of the Basin as Seen by Data from the Lunar Reconnaissance Orbiter (LRO)

    NASA Astrophysics Data System (ADS)

    Petro, N. E.; Jolliff, B. L.; Cahill, J. T.; Whelley, P.

    2015-12-01

    The interior of SPA contains a range of morphologic units, from smooth plains and mare basalts to rough, ancient, terrains. Recent data, particularly from LRO provide unique measures of SPA surface properties. With each new dataset, the differences between the interior of SPA and its surroundings become more, or in some cases less, clearly defined. Here we explore recent datasets that offer insight into surface roughness at a variety of scales and assess implications for the origins of units across SPA. Identifying the origin of units in SPA is critical for identifying future sampling sites that address the science goal of determining the age of SPA. The unique interior of SPA relative to the rest of the Moon is demonstrated by Mini-RF and LOLA derived products. Mini-RF data shows that the interior of SPA has a slightly higher average Circular Polarization Ratio than nearly any other terrain on the Moon, with the exception of the interior of the Orientale Basin. Cahill et al. [2014,Icarus] note that the average interior CPR value of SPA is similar but slightly higher than the mid-latitude farside highlands, suggesting that both are enhanced in blocks at the surface and near subsurface (to depths <2m). However, SPA is distinct relative to the interior of Orientale in the m-chi deconvolution, suggesting that the interior has a regolith with scatterers smaller than the wavelength of Mini-RF (12.6cm). LOLA-derived roughness shows that the interior of SPA is smoother than the surrounding highlands [Rosenburg et al.,2011,JGR; Kreslavsky et al., 2013,Icarus]. LOLA roughness measures show roughness variations within the basin tied to volcanism. However the expanse of smooth areas is broader than the previously mapped volcanic units, suggesting that ancient volcanism may be contributing to interior smoothness. The distinction between datasets is likely due to differences in spatial resolution and that Mini-RF data probes the near subsurface. These measurements of roughness

  5. Topographic-Compositional Relationships within the South Pole Aitken Basin

    NASA Astrophysics Data System (ADS)

    Lucey, P. G.; Holtzmann, J.; Blewett, D. T.; Taylor, G. J.; Hawke, B. R.

    1999-01-01

    The South Pole Aitken (SPA) Basin is an immense structure that dominates the geology of much of the farside of the Moon. Its floor is composed mostly of impact deposits, though it also has numerous relatively small regions of mare basalt. The basin floor exhibits a lower albedo and higher mafic mineral abundance than the surrounding highlands [ I ]. The origin of this mafic anomaly is a major question in lunar geology. Hypotheses for the presence of the mafic anomaly were briefly reviewed in [2] and include mare deposits mixed and obscured by basin or crater ejecta (cryptomaria), a large impact melt sheet that may have differentiated, exposed lower crustal material, and a significant component of excavated mantle. A study of mineralogy as revealed in Clementine UV-VIS imagery for limited portions of the basin found a predominantly low-Capyroxene (noritic) character [2], ruling out cryptomaria as an important contributor to the mafic enhancement. A few small cryptomaria, revealed by dark-halo impact craters and light plains units with high-FeO contents, have been found in SPA; however, it appears that extensive cryptomaria are lacking in this basin. The uniformly noritic lithology within SPA led to favor exposed lower crust or a homogenized melt sheet as the explanation for the mafic anomaly. Models of basin formation predict that a basin the size of SPA should have excavated through the entire lunar crust (assuming nonoblique impact), potentially exposing or mixing a large component of material from the mantle. Comparison of SPA floor FeO and Ti02 (derived from Clementine UV-VIS observations) and also Th (from Lunar Prospector) with model-mantle chemistries appears to be consistent with a mixture of approximately equal proportions of lower-crust and mantle material. In the present study, we examine the relationship between the basin's topography and composition in order to provide further insight on the origin of the basin floor material. Data: Clementine UV

  6. Regional elemental abundances within South Pole-Aitken basin as measured with lunar prospector gamma-ray spectrometer data.

    SciTech Connect

    Lawrence, David J. ,; Pieters, Carlé M.; Elphic, R. C.; Gasnault, O. M.; Prettyman, T. H.; Feldman, W. C.

    2003-01-01

    South Pole-Aitken (SPA) basin has been a target of intense study since it is one of the largest impact basins in the solar system. It is thought that SPA basin excavated deep into the lunar crust and possibly even the mantle. Such conclusions have been supported by the observed mafic and thorium composition anomalies seen across the entire basin. One of the major goals of lunar and planetary science has been to measure and understand the composition of the non-mare materials within SPA basin. It is expected that this information will help to increase our understanding of the formation and differentiation processes that occurred early on the Moon.

  7. Potential sample sites for South Pole-Aitken basin impact melt within the Schrödinger basin

    NASA Astrophysics Data System (ADS)

    Hurwitz, Debra; Kring, David A.

    2015-10-01

    Determining the age of the South Pole-Aitken (SPA) basin ranks among the highest priorities in lunar science. This datum would constrain the timing of the oldest and largest basin-forming event on the Moon, information that is essential to any evaluation of the collisional evolution of the early Solar System. To locate material that preserves the age of SPA, a geochemical model of SPA impact melt is integrated with chemical and mineralogical analyses of the lunar surface determined from orbit. Results suggest the southern wall of Schrödinger basin contains material with the mineralogical and geochemical signatures of SPA melt and, thus, represents a candidate destination for sampling material that can constrain the age of the SPA impact.

  8. Volume of Impact Melt Generated by the Formation of the South Pole-Aitken Basin

    NASA Technical Reports Server (NTRS)

    Petro, Noah E.

    2011-01-01

    The South Pole-Aitken Basin (SPA) is the largest, deepest, and oldest identified basin on the Moon and as such contains surfaces that are unique due to their age, composition, and depth of origin in the lunar crust [1-5] (Figure 1). SPA has been a target of intense interest as an area for robotic sample return in order to determine the age of the basin and the composition and origin of its interior [6-8]. In response to this interest there have been several efforts to estimate the likely provenance of regolith material within central SPA [9-12]. These model estimates suggest that, despite the formation of basins and craters following SPA, the regolith within SPA is dominated by locally derived material. An assumption of these models has been that the locally derived material is primarily SPA impact-melt as opposed to local basement material (e.g. unmelted lower crust). However, the definitive identification of SPA derived impact melt on the basin floor, either by remote sensing [5, 13] or via photogeology [2, 14] is extremely difficult due to the number of subsequent impacts and volcanic activity [4].

  9. Mineralogy of the Mafic Anomaly in the South Pole-Aitken Basin: Implications for excavation of the lunar mantle

    NASA Technical Reports Server (NTRS)

    Pieters, C. M.; Tompkins, S.; Head, J. W.; Hess, P. C.

    1997-01-01

    Mineralogy of South Pole-Aitken Basin (SPA) (the largest confirmed impact basin on the Moon) is evaluated using five-color images from Clementine. Although olivine-rich material as well as basalts rich in clinopyroxene are readily identified elsewhere on the farside, the dominant rock type observed across the interior of SPA is of a very noritic composition. This mineralogy suggests that lower crust rather than the mantle is the dominant source of the mafic component at SPA. The lack of variation in observed noritic composition is probably due to basin formation processes, during which extensive melting and mixing of target materials are likely to occur.

  10. Sample Return Mission to the South Pole Aitken Basin

    NASA Astrophysics Data System (ADS)

    Duke, M. B.; Clark, B. C.; Gamber, T.; Lucey, P. G.; Ryder, G.; Taylor, G. J.

    1999-01-01

    The South Pole Aitken Basin (SPA) is the largest and oldest observed feature on the Moon. Compositional and topographic data from Galileo, Clementine, and Lunar Prospector have demonstrated that SPA represents a distinctive major lunar terrane, which has not been sampled either by sample return missions (Apollo, Luna) or by lunar meteorites. The floor of SPA is characterized by mafic compositions enriched in Fe, Ti, and Th in comparison to its surroundings. This composition may represent melt rocks from the SPA event, which would be mixtures of the preexisting crust and mantle rocks. However, the Fe content is higher than expected, and the large Apollo basin, within SPA, exposes deeper material with lower iron content. Some of the Fe enrichment may represent mare and cryptomare deposits. No model adequately accounts for all of the characteristics of the SPA and disagreements are fundamental. Is mantle material exposed or contained as fragments in melt rock and breccias? If impact melt is present, did the vast sheet differentiate? Was the initial mantle and crust compositionally different from other regions of the Moon? Was the impact event somehow peculiar, (e.g., a low-velocity impact)? The precise time of formation of the SPA is unknown, being limited only by the initial differentiation of the Moon and the age of the Imbrium event, believed to be 3.9 b.y. The questions raised by the SPA can be addressed only with detailed sample analysis. Analysis of the melt rocks, fragments in breccias, and basalts of SPA can address several highly significant problems for the Moon and the history of the solar system. The time of formation of SPA, based on analysis of melt rocks formed in the event. would put limits on the period of intense bombardment of the Moon, which has been inferred by some to include a "terminal cataclysm." If close to 3.9 Ga, the presumed age of the Imbrium Basin, the SPA date would confirm the lunar cataclysm. This episode, if it occurred, would have

  11. The nature and origin of Mafic Mound in the South Pole-Aitken Basin

    NASA Astrophysics Data System (ADS)

    Moriarty, Daniel P.; Pieters, Carle M.

    2015-10-01

    "Mafic Mound" is a distinctive and enigmatic feature 75 km across and 1 km high near the center of the vast South Pole-Aitken Basin (SPA). Using several modern data sets, we characterize the composition, morphology, and gravity signature of the structure in order to assess its origin. Mafic Mound is found to exhibit a perched circular depression and a homogeneous high-Ca pyroxene-bearing composition. Several formation hypotheses based on known lunar processes are evaluated, including the possibilities that Mafic Mound represents (1) uplifted mantle, (2) SPA-derived impact melt, (3) a basalt-filled impact crater, or (4) a volcanic construct. Individually, these common processes cannot fully reproduce the properties of Mafic Mound. Instead, we propose a hybrid origin in which Mafic Mound is an edifice formed by magmatic processes induced by the formation and evolution of SPA. This form of nonmare volcanism has not previously been documented on the Moon.

  12. Moonrise: Sampling the South Pole-Aitken Basin to Address Problems of Solar System Significance

    NASA Technical Reports Server (NTRS)

    Zeigler, R. A.; Jolliff, B. L.; Korotev, R. L.; Shearer, C. K.

    2016-01-01

    A mission to land in the giant South Pole-Aitken (SPA) Basin on the Moon's southern farside and return a sample to Earth for analysis is a high priority for Solar System Science. Such a sample would be used to determine the age of the SPA impact; the chronology of the basin, including the ages of basins and large impacts within SPA, with implications for early Solar System dynamics and the magmatic history of the Moon; the age and composition of volcanic rocks within SPA; the origin of the thorium signature of SPA with implications for the origin of exposed materials and thermal evolution of the Moon; and possibly the magnetization that forms a strong anomaly especially evident in the northern parts of the SPA basin. It is well known from studies of the Apollo regolith that rock fragments found in the regolith form a representative collection of many different rock types delivered to the site by the impact process (Fig. 1). Such samples are well documented to contain a broad suite of materials that reflect both the local major rock formations, as well as some exotic materials from far distant sources. Within the SPA basin, modeling of the impact ejection process indicates that regolith would be dominated by SPA substrate, formed at the time of the SPA basin-forming impact and for the most part moved around by subsequent impacts. Consistent with GRAIL data, the SPA impact likely formed a vast melt body tens of km thick that took perhaps several million years to cool, but that nonetheless represents barely an instant in geologic time that should be readily apparent through integrated geochronologic studies involving multiple chronometers. It is anticipated that a statistically significant number of age determinations would yield not only the age of SPA but also the age of several prominent nearby basins and large craters within SPA. This chronology would provide a contrast to the Imbrium-dominated chronology of the nearside Apollo samples and an independent test of

  13. Sample return from the lunar South Pole-Aitken Basin

    NASA Astrophysics Data System (ADS)

    Duke, M. B.

    2003-06-01

    Automated sample return missions to the South Pole — Aitken Basin on the lunar far side are proposed as the best means of addressing major problems concerning the early impact history of the inner solar system, the nature of very large impact events, and the early differentiation of rocky planets. The opportunity to propose such missions has been opened by the recommendations of the U. S. National Research Council's Decadal Study of Solar System Exploration and the creation by NASA of the New Frontiers Program, which will support missions of intermediate cost, between the Discovery Program and large missions. A proposal for a South Pole — Aitken Basin Sample Return Mission was submitted to the Discovery Program in 2000, but not fimded. The New Frontiers Program, with a somewhat less stringent budget constraint, should allow several of the potential risks associated with the Discovery proposal to be addressed, including scientific and programmatic risks. A principal goal of current mission studies is to determine whether, within the New Frontiers Program's cost constraints, two separate samples could be collected from areas of different post-Basin geological history. If accepted by the New Frontiers Program, a South Pole — Aitken Basin sample return mission could be flown as early as 2008-2009.

  14. Rock types of South Pole-Aitken basin and extent of basaltic volcanism

    USGS Publications Warehouse

    Pieters, C.M.; Head, J. W.; Gaddis, L.; Jolliff, B.; Duke, M.

    2001-01-01

    The enormous pre-Nectarian South Pole-Aitken (SPA) basin represents a geophysically and compositionally unique region on the Moon. We present and analyze the mineralogical diversity across this basin and discuss the implications for basin evolution. Rock types are derived from Clementine multispectral data based on diagnostic characteristics of ferrous absorptions in fresh materials. Individual areas are characterized as noritic (dominated by low-Ca pyroxene), gabbroic/basaltic (dominated by high-Ca pyroxene), feldspathic (<3-6% FeO), and olivine-gabbro (dominated by high-Ca pyroxene and olivine). The anorthositic crust has effectively been removed from the interior of the basin. The style of volcanism within the basin extends over several 100 Myr and includes mare basalt and pyroclastic deposits. Several areas of ancient (pre-Orientale) volcanism, or cryptomaria, have also been identified. The nonmare mafic lithology that occurs across the basin is shown to be noritic in composition and is pervasive laterally and vertically. We interpret this to represent impact melt/breccia deposits derived from the lower crust. A few localized areas are identified within the basin that contain more diverse lithologies (gabbro, olivine-gabbro), some of which may represent material from the deepest part of the lower crust and perhaps uppermost mantle involved in the SPA event. Copyright 2001 by the American Geophysical Union.

  15. Magnetic Signature of the Lunar South Pole-Aitken Basin: Character, Origin, and Age

    NASA Technical Reports Server (NTRS)

    Purucker, Michael E.; Head, James W., III; Wilson, Lionel

    2012-01-01

    A new magnetic map of the Moon, based on Lunar Prospector (LP) magnetometer observations, sheds light on the origin of the South Pole-Aitken Basin (SPA), the largest and oldest of the recognized lunar basins. A set of WNW-trending linear to arcuate magnetic features, evident in both the radial and scalar observations, covers much of a 1000 km wide region centered on the NW portion of SPA. The source bodies are not at the surface because the magnetic features show no first-order correspondence to any surface topographic or structural feature. Patchy mare basalts of possible late Imbrianage are emplaced within SPA and are inferred to have been emplaced through dikes, directly from mantle sources. We infer that the magnetic features represent dike swarms that served as feeders for these mare basalts, as evident from the location of the Thomson/ Mare Ingenii, Van de Graaff, and Leeuwenhoek mare basalts on the two largest magnetic features in the region. Modeling suggests that the dike zone is between 25 and 50 km wide at the surface, and dike magnetization contrasts are in the range of 0.2 A/m. We theorize that the basaltic dikes were emplaced in the lunar crust when a long-lived dynamo was active. Based on pressure, temperature, and stress conditions prevalent in the lunar crust, dikes are expected to be a dominantly subsurface phenomenon, consistent with the observations reported here.

  16. Thorium Anomalies in the NW Quadrant of the South Pole-Aitken Basin

    NASA Technical Reports Server (NTRS)

    Haskin, Larry A.; McKinnon, William B.; Benner, Lance A. M.; Jolliff, Bradley L.

    2004-01-01

    The relatively high concentrations of Th near the Imbrium antipode in the South Pole-Aitken (SPA) basin might represent Imbrium ejecta, a consequence of convergence of Th-rich material ejected by the Imbrium impact that occurred in the Th-rich Procellarum KREEP Terrane. Here, we present landing positions for 7500 fragments ejected from Imbrium obtained by three-body (Earth-Moon-fragment) numerical integration for uniformly selected azimuthal launch positions, ejection angles of 45 deg, and velocities from 0.95 to 0.99 lunar escape. This provides an estimate of the density of infalling ejecta fragments to be expected in the vicinity of the Imbrium antipode. Similar calculations for 35 and 50 deg leave large empty regions surrounding the antipode.

  17. South Pole-Aitken Basin: Evidence for Post-Basin Resurfacing from Lunar Orbiter Laser Altimeter (LOLA) Data

    NASA Astrophysics Data System (ADS)

    Head, J. W.; Fassett, C.; Kadish, S.; Smith, D. E.; Zuber, M. T.; Neumann, G. A.; Mazarico, E.

    2010-12-01

    The lunar farside South Pole-Aitken Basin is the largest and oldest documented basin on the Moon and is thus of interest from the point of view of the scale of production of impact melt at large basin-event sizes and its ring structure and potential depth of sampling at such a large diameter. We used new LOLA data from the Lunar Reconnaissance Orbiter 1) to characterize the basin interior topography, 2) to assess the nature of the nearby and relatively pristine Orientale basin and compare it to the SPA interior, and 3) to compile a new global crater database of all lunar craters ≥20 km in diameter and to assess the population of impact craters superposed on the SPA interior and exterior. We find that impact crater size-frequency distribution plots show that the exterior of the SPA basin is similar to the most heavily cratered regions of the Moon, but that the interior of the basin has a deficiency of craters in the 20-64 km diameter crater range. One interpretation of these data is that some resurfacing process (or processes) has modified the superposed crater population. Among the candidates are 1) impact crater proximity weathering/degradation by adjacent (e.g., Apollo) and nearby (e.g., Orientale) impact basin ejecta, 2) volcanic resurfacing by early non-mare volcanism, cryptomaria and/or maria, and 3) viscous relaxation removing crater topography. We consider viscous relaxation of crater topography to be the least likely due to the wavelength dependence of the process (rim-crests should be preserved and thus detected in our crater counts). Careful analysis of the impact ejecta thickness radial decay suggests that it is an important resurfacing mechanism within a basin radius from the rim crest, but is unlikely to be sufficient to explain the observed deficiency. Morphometric analysis of impact craters, modeling, and simulations of volcanic flooding suggest that the deficiency may be related to the patchy distribution of cryptomaria, suspected from mineralogic

  18. Geological and geochemical analysis of stratigraphic units in the South Pole - Aitken Basin

    NASA Astrophysics Data System (ADS)

    Borst, A.; Bexkens, F.; Foing, B.; Koschny, D.; Davies, G.; van Westrenen, W.

    2009-04-01

    The South Pole-Aitken (SPA) Basin, located on the Lunar far side, is one of the oldest and largest recognized impact structure in the solar system. This PreNectarian basin (>3.9 Ga) measures 2500 km in diameter with depths up to 13 km. A large mafic province was formed by the impact that effectively removed the upper crust [1]. Hence, deep-seated lower crustal and possibly even mantle materials are exposed in the severely modified Basin interior, providing the unique opportunity to probe and study the composition and structure of the Lunar interior. Consequently, the SPA Basin is a frequently proposed site for future sample return missions and detailed multispectral studies will required to aid landing site selection [2]. Previous studies on the multispectral dataset of Clementine (1994) by Pieters and Tompkins [1,3] revealed fresh mafic compositions of both low-Ca pyroxene or high-Ca pyroxene dominated rocks, referred to as norites and gabbros respectively. Some regions contained spectral features of olivine (troctolite), such as in Olivine Hill, which could suggest the presence of mantle derived deposits tapped during SPA impact. Using an algorithm developed by Pieters et al. [1] we have produced images for three subregions, covering the central and northern part of the SPA Basin. The algorithm is based on three diagnostic features in the UV/VIS spectrum of Clementine's 11 band multispectral dataset. The parameters are assigned to an RGB composite and allow distinction between mature soils, anorthosite (blue), norite (pink) and gabbro/troctolite compositions (green). Furthermore, we have used Clementine's Near Infrared database to produce a NIR band ratio image (2000 nm/1250 nm), as a parameter to distinguish between olivine and pyroxene-rich materials where we aim to detect traces of excavated mantle material (modified from LeMoeulic et al. [4]). Regretfully, we found that the NIR ratio method does not confirm olivine-rich material exposed in Olivine Hill as it

  19. Formation of South Pole-Aitken Basin as the Result of an Oblique Impact: Implications for Melt Volume and Source of Exposed Materials

    NASA Technical Reports Server (NTRS)

    Petro, N. E.

    2012-01-01

    The South Pole-Aitken Basin (SPA) is the largest, deepest, and oldest identified basin on the Moon and contains surfaces that are unique due to their age, composition, and depth of origin in the lunar crust [1-3] (Figure 1). SPA has been a target of interest as an area for robotic sample return in order to determine the age of the basin and the composition and origin of its interior [3-6]. As part of the investigation into the origin of SPA materials there have been several efforts to estimate the likely provenance of regolith material in central SPA [5, 6]. These model estimates suggest that, despite the formation of basins and craters following SPA, the regolith within SPA is dominated by locally derived material. An assumption inherent in these models has been that the locally derived material is primarily SPA impact-melt as opposed to local basement material (e.g. unmelted lower crust). However, the definitive identification of SPA derived impact melt on the basin floor, either by remote sensing [2, 7] or via photogeology [8] is extremely difficult due to the number of subsequent impacts and volcanic activity [3, 4]. In order to identify where SPA produced impact melt may be located, it is important to constrain both how much melt would have been produced in a basin forming impact and the likely source of such melted material. Models of crater and basin formation [9, 10] present clear rationale for estimating the possible volumes and sources of impact melt produced during SPA formation. However, if SPA formed as the result of an oblique impact [11, 12], the volume and depth of origin of melted material could be distinct from similar material in a vertical impact [13].

  20. Local Lunar Gravity Field Analysis over the South Pole-aitken Basin from SELENE Farside Tracking Data

    NASA Technical Reports Server (NTRS)

    Goossens, Sander Johannes; Ishihara, Yoshiaki; Matsumoto, Koji; Sasaki, Sho

    2012-01-01

    We present a method with which we determined the local lunar gravity field model over the South Pole-Aitken (SPA) basin on the farside of the Moon by estimating adjustments to a global lunar gravity field model using SELENE tracking data. Our adjustments are expressed in localized functions concentrated over the SPA region in a spherical cap with a radius of 45deg centered at (191.1 deg E, 53.2 deg S), and the resolution is equivalent to a 150th degree and order spherical harmonics expansion. The new solution over SPA was used in several applications of geophysical analysis. It shows an increased correlation with high-resolution lunar topography in the frequency band l = 40-70, and admittance values are slightly different and more leveled when compared to other, global gravity field models using the same data. The adjustments expressed in free-air anomalies and differences in Bouguer anomalies between the local solution and the a priori global solution correlate with topographic surface features. The Moho structure beneath the SPA basin is slightly modified in our solution, most notably at the southern rim of the Apollo basin and around the Zeeman crater

  1. Characterisation of Landing Sites at the Rim of the Lunar South Pole Aitken Basin

    NASA Astrophysics Data System (ADS)

    Koebel, David; Bonerba, Michele; Wieser, Matthias; Homeister, Maren; Borowy, Carsten

    The lunar South polar region is of high scientific interest and advantageous in many aspects for exploration missions. The polar terrain is located at the rim of the Aitken Basin, being the largest impact crater in the solar system, where material from the lunar mantle has been ejected to the surface. This basin features a diameter of 2,500 km and variations in altitude as large as 14 km. Since the solar elevation never exceeds 1.5 at the pole, there exist mountain peaks in this area that are characterised by near eternal illumination. These summits provide a benign thermal environment for any long-term robotic or manned lander mission, and ideal conditions for photovoltaic power generation. The smaller impact craters in the polar region, on the other hand, possess depths with constant darkness. These craters are evidently harbouring water resources that remain conserved through the cryogenic temperatures inside them. The ice originates from the bombardment of comets throughout the billions of years after the formation of the lunar crust. For this terrain updated analyses of the solar illumination and ground station visibility conditions have been performed. These are based on the refined lunar digital elevation model provided by the Japanese Kaguya/Selene mission, originating from its LASER altimeter instrument. The resulting maps for the South polar region will be presented in this paper. Some considerations on the geology of interesting locations within the SPA are complemented. With these prerequisites, several possible landing sites for a future lunar mission have been selected. A detailed analysis of illumination timelines will be presented for these sites.

  2. Pulling Marbles from a Bag: Deducing the Regional Impact History of the SPA Basin from Impact-Melt Rocks

    NASA Technical Reports Server (NTRS)

    Cohen, Barbara A.; Coker, Robert F.

    2010-01-01

    The South Pole Aitken (SPA) basin is the stratigraphically oldest identifiable lunar basin and is therefore one of the most important targets for absolute age-dating to help understand whether ancient lunar bombardment history smoothly declined or was punctuated by a cataclysm. A feasible near-term approach to this problem is to robotically collect a sample from near the center of the basin, where vertical and lateral mixing provided by post-basin impacts ensures that such a sample will be composed of small rock fragments from SPA itself, from local impact craters, and from faraway giant basins. The range of ages, intermediate spikes in the age distribution, and the oldest ages are all part of the definition of the absolute age and impact history recorded within the SPA basin.

  3. Lunar Reconnaissance Orbiter Camera Observations Relating to Science and Landing Site Selection in South Pole-Aitken Basin for a Robotic Sample Return Mission

    NASA Technical Reports Server (NTRS)

    Jolliff, B. L.; Clegg-Watkins, R. N.; Petro, N. E.; Lawrence, S. L.

    2016-01-01

    The Moon's South Pole-Aitken basin (SPA) is a high priority target for Solar System exploration, and sample return from SPA is a specific objective in NASA's New Frontiers program. Samples returned from SPA will improve our understanding of early lunar and Solar System events, mainly by placing firm timing constraints on SPA formation and the post-SPA late-heavy bombardment (LHB). Lunar Reconnaissance Orbiter Camera (LROC) images and topographic data, especially Narrow Angle Camera (NAC) scale (1-3 mpp) morphology and digital terrain model (DTM) data are critical for selecting landing sites and assessing landing hazards. Rock components in regolith at a given landing site should include (1) original SPA impact-melt rocks and breccia (to determine the age of the impact event and what materials were incorporated into the melt); (2) impact-melt rocks and breccia from large craters and basins (other than SPA) that represent the post-SPA LHB interval; (3) volcanic basalts derived from the sub-SPA mantle; and (4) older, "cryptomare" (ancient buried volcanics excavated by impact craters, to determine the volcanic history of SPA basin). All of these rock types are sought for sample return. The ancient SPA-derived impact-melt rocks and later-formed melt rocks are needed to determine chronology, and thus address questions of early Solar System dynamics, lunar history, and effects of giant impacts. Surface compositions from remote sensing are consistent with mixtures of SPA impactite and volcanic materials, and near infrared spectral data distinguish areas with variable volcanic contents vs. excavated SPA substrate. Estimating proportions of these rock types in the regolith requires knowledge of the surface deposits, evaluated via morphology, slopes, and terrain ruggedness. These data allow determination of mare-cryptomare-nonmare deposit interfaces in combination with compositional and mineralogical remote sensing to establish the types and relative proportions of materials

  4. The GIS-based geologic investigation of the South Pole-Aitken basin region of the Moon using SELENE elemental information

    NASA Astrophysics Data System (ADS)

    Kim, K. J.; Dohm, J. M.; Williams, J.; Ruiz, J.; Yu, B.; Hare, T. M.; Hasebe, N.; Yamashita, N.; Karouji, Y.; Kobayashi, S.; Hareyama, M.; Shibamura, E.; Kobayashi, M.; D'Uston, C.; Gasnault, O.; Forni, O.; Reedy, R. C.

    2010-12-01

    Using Geographic Information Systems (GIS), we performed comparative analysis among stratigraphic information and the Kaguya (SELENE) GRS data of the South Pole-Aitken (SPA) basin and its surroundings. Results indicate that the rock materials up to ~ 1m depth (including ancient crater materials, mare basalts, and possible SPA impact melt) are average to slightly above average in K and Th with respect to the rest of the Moon. The heavily cratered highlands outside of SPA represent ancient deep-seated crustal and possibly mantle igneous materials harvested in part from the giant SPA impact event as ejecta, as well as subsequent impact cratering events up until the end of the Late Heavy Bombardment, which includes intensive impact-related mixing of ejecta materials and lava flows. The geologic history of the SPA basin is distinct from the Procellarum-Imbrium region. The former records mainly pre-Nectarian activity such as the giant impact with minor mare volcanism during the Upper Imbriam, whereas the latter was largely resurfaced by activity such as the Imbrium impact event and subsequent emplacement of voluminous mare-forming lavas during the Lower Imbriam and Upper Imbriam, Eraatosthenian, and Copernican, respectively. These distinct geologic histories bear on the mineralogic and elemental abundances, as shown in our investigation through this GIS-based comparative analysis among the stratigraphic and Kaguya (SELENE) GRS data.

  5. Imaging the South Pole-Aitken basin in backscattered neutral hydrogen atoms

    NASA Astrophysics Data System (ADS)

    Vorburger, A.; Wurz, P.; Barabash, S.; Wieser, M.; Futaana, Y.; Bhardwaj, A.; Asamura, K.

    2015-09-01

    The lunar surface is very efficient in reflecting impinging solar wind ions as energetic neutral atoms (ENAs). A global analysis of lunar hydrogen ENAs showed that on average 16% of the solar wind protons are reflected, and that the reflected fraction can range from less than 8% to more than 24%, depending on location. It is established that magnetic anomalies reduce the flux of backscattered hydrogen ENAs by screening-off a fraction of the impinging solar wind. The effects of the surface properties, such as porosity, roughness, chemical composition, and extent of weathering, were not known. In this paper, we conduct an in-depth analysis of ENA observations of the South Pole-Aitken basin to determine which of the surface properties might be responsible for the observed variation in the integral ENA flux. The South Pole-Aitken basin with its highly variable surface properties is an ideal object for such studies. It is very deep, possesses strikingly elevated concentrations in iron and thorium, has a low albedo and coincides with a cluster of strong magnetic anomalies located on the northern rim of the basin. Our analysis shows that whereas, as expected, the magnetic anomalies can account well for the observed ENA depletion at the South Pole-Aitken basin, none of the other surface properties seem to influence the ENA reflection efficiency. Therefore, the integral flux of backscattered hydrogen ENAs is mainly determined by the impinging plasma flux and ENA imaging of backscattered hydrogen captures the electrodynamics of the plasma at the surface. We cannot exclude minor effects by surface features. We create two maps of surface reflected ENAs at the South Pole-Aitken basin. We compare these ENA maps to elevation, albedo, composition and magnetic field maps. The ENA maps only significantly correlate with the magnetic field map. ENA imaging captures solely the electrodynamics of the plasma at the surface.

  6. Thorium abundances of basalt ponds in South Pole-Aitken basin: Insights into the composition and evolution of the far side lunar mantle

    USGS Publications Warehouse

    Hagerty, J.J.; Lawrence, D.J.; Hawke, B.R.

    2011-01-01

    Imbrian-aged basalt ponds, located on the floor of South Pole-Aitken (SPA) basin, are used to provide constraints on the composition and evolution of the far side lunar mantle. We use forward modeling of the Lunar Prospector Gamma Ray Spectrometer thorium data, to suggest that at least five different and distinct portions of the far side lunar mantle contain little or no thorium as of the Imbrian Period. We also use spatial correlations between local thorium enhancements and nonmare material on top of the basalt ponds to support previous assertions that lower crustal materials exposed in SPA basin have elevated thorium abundances, consistent with noritic to gabbronoritic lithologies. We suggest that the lower crust on the far side of the Moon experienced multiple intrusions of thorium-rich basaltic magmas, prior to the formation of SPA basin. The fact that many of the ponds on the lunar far side have elevated titanium abundances indicates that the far side of the Moon experienced extensive fractional crystallization that likely led to the formation of a KREEP-like component. However, because the Imbrian-aged basalts contain no signs of elevated thorium, we propose that the SPA impact event triggered the transport of a KREEP-like component from the lunar far side and concentrated it on the nearside of the Moon. Because of the correlation between basaltic ponds and basins within SPA, we suggest that Imbrian-aged basaltic volcanism on the far side of the Moon was driven by basin-induced decompressional melting. Copyright ?? 2011 by the American Geophysical Union.

  7. Apollo Basin, Moon: Estimation of Impact Conditions

    NASA Astrophysics Data System (ADS)

    Echaurren, J. C.

    2015-07-01

    The Apollo Basin is a, pre-Nectarian, multi-ring basin located within the large South Pole-Aitken Basin (SPA). Multispectral data from both Galileo and Clementine showed that the composition of materials in Apollo is distinct…

  8. Pulling Marbles from a Bag: Deducing the Regional Impact History of the SPA Basin from Impact Melt Rocks

    NASA Technical Reports Server (NTRS)

    Cohen, Barbara A.; Coker, R. F.

    2009-01-01

    The South Pole-Aitken (SPA) basin is an important target for absolute age-dating. Vertical and lateral impact mixing ensures that regolith within SPA will contain rock fragments from SPA itself, local impact craters, and faraway giant basins. About 20% of the regolith at any given site is foreign [1, 2], but much of this material will be cold ejecta, not impact melt. We calculated the fraction of contributed impact melt using scaling laws to estimate the amount and provenance of impact melt, demonstrating that SPA melt is the dominant impact melt rock (>70%) likely to be present. We also constructed a statistical model to illustrate how many randomly-selected impact-melt fragments would need to be dated, and with what accuracy, to confidently reproduce the impact history of a site. A detailed impact history becomes recognizable after a few hundred to a thousand randomly-selected marbles, however, it will be useful to have more information (e.g. compositional, mineralogical, remote sensing) to group fragments. These exercises show that SPA melt has a high probability of being present in a scoop sample and that dating of a few hundred to a thousand impact-melt fragments will yield the impact history of the SPA basin.

  9. Geomorphic Terrains and Evidence for Ancient Volcanism within Northeastern South Pole-Aitken Basin

    NASA Technical Reports Server (NTRS)

    Petro, Noah; Mest, Scott C.; Teich, Yaron

    2010-01-01

    The interior of the enigmatic South Pole-Aitken Basin has long been recognized as being compositionally distinct from its exterior. However, the source of the compositional anomaly has been subject to some debate. Is the source of the iron-enhancement due to lower-crustal/upper-mantle material being exposed at the surface, or was there some volume of ancient volcanism that covered portions of the basin interior? While several obvious mare basalt units are found within the basin and regions that appear to represent the original basin interior, there are several regions that appear to have an uncertain origin. Using a combination of Clementine and Lunar Orbiter images, several morphologic units are defined based on albedo, crater density, and surface roughness. An extensive unit of ancient mare basalt (cryptomare) is defined and, based on the number of superimposed craters, potentially represents the oldest volcanic materials within the basin. Thus, the overall iron-rich interior of the basin is not solely due to deeply derived crustal material, but is, in part due to the presence of ancient volcanic units.

  10. Imaging the South Pole - Aitken Basin in Backscattered Energetic Neutral Hydrogen Atoms

    NASA Astrophysics Data System (ADS)

    Vorburger, Audrey; Wurz, Peter; Barabash, Stas; Wieser, Martin; Futaana, Yoshifumi; Bhardwaj, Anil; Asamura, Kazushi

    2015-04-01

    The Moon, not being protected by a global magnetic field or an atmosphere, is constantly bombarded by solar wind ions. Until a few years ago, it was tacitly assumed that the impinging solar wind ions are almost completely absorbed ( < 1% reflection) by the lunar surface (e.g. Crider and Vondrak, Adv. Space Res., (2002); Feldman et al., J. Geophys. Res., (2000)). Recent observations by IBEX and the Sub-keV Atom Reflecting Analyzer (SARA) onboard Chandrayaan-1 invalidated this assumption, though: In fact, these measurements showed that the lunar surface very efficiently reflects impinging solar wind ions as energetic neutral atoms (ENAs) (e.g. McComas et al., GRL, 2009; Wieser et al., PSS, 2009). Most recently, a global analysis of lunar hydrogen ENAs measured by SARA showed that on average 16% of the solar wind protons are reflected, and that the reflected fraction can range from less than 8% to more than 24%, depending on location (Vorburger et al., J. Geophys. Res., 2013). Whereas it is established that magnetic anomalies reduce the flux of backscattered hydrogen ENAs by screening-off a fraction of the impinging solar wind ions (e.g. Wieser et al., Planet. Space Sci., (2009); Lue et al., Geophys. Res. Lett., (2011); Vorburger et al., J. Geophys. Res., (2012); Futaana et al., Geophys. Res. Lett., (2013)), the effects of other surface properties such as porosity, roughness, chemical composition, and extent of weathering, was not known. To investigate the effects of these surface properties on the properties of scattered ENAs, we conducted an in-depth analysis of ENA observations near the South Pole - Aitken basin using the complete dataset collected by SARA. The South Pole - Aitken basin is an ideal object for such a study, because it highly differs in many properties from the surrounding terrain. It is very deep (~13 km), possesses strikingly elevated concentrations in iron (~15 wt%) and thorium (~7 wt%), has a low albedo and coincides with a cluster of strong

  11. Thorium Abundances of Basalt Ponds in South Pole-Aitken Basin: Insights into the Composition and Evolution of the Far Side Lunar Mantle

    NASA Astrophysics Data System (ADS)

    Hagerty, J. J.; Lawrence, D. J.; Hawke, B. R.

    2011-03-01

    We used forward modeling of Lunar Prospector thorium (Th) data derived from basalts in South Pole-Aitken basin to demonstrate that large expanses of basalt could be reliably used to obtain compositional information about the far side lunar mantle.

  12. GRAIL Investigation of the Subsurface Structure of South Pole-Aitken Basin

    NASA Astrophysics Data System (ADS)

    Ertel, A.; Nimmo, F.; Besserer, J.; Hurwitz, D. M.

    2014-12-01

    The GRAIL mission [1] has revealed a lunar crust that is globally less dense and presumably more porous [2] than anticipated. Measurements of the inferred (effective) density as a function of wavelength provide a way of probing the vertical density structure of the crust. Both the mean vertical density stratification [3] and spatial variations in the density structure [4] have been determined. Here we used an admittance approach [4] to investigate the subsurface structure of South Pole-Aikten (SPA) Basin. The SPA-forming impact was large enough to generate enormous quantities of melt [5,6] and a global ejecta layer [7]. The pre-existing crust was probably completely removed [8]; GRAIL data indicate a depth to the base of the crust (or other density interface) at a depth of about 13-20 km beneath the basin center [2]. Cooling and crystallization of the melt pool will have generated a distinct stratigraphy and density structure [6,5]. This predicted density structure can then be compared to that inferred from the GRAIL observations. We determined the effective density spectrum of SPA using a localized multitaper approach [4]. The effective density decreases from 2.7 g/cc at spherical harmonic degree l=250 to 2.6 g/cc at l=550, indicating an increase in density with depth. Two model predictions from [5] yield effective densities in the range 3.0-3.2 g/cc. The discrepancy between the predictions and the observations could be explained by 1) incorporations of large volumes of crustal material into the recrystallizing melt-sheet; or 2) ~20% fracture porosity extending to depths of 10 km or more. The latter possibility is more likely given SPA early formation and subsequent reprocessing and delivery of low-density material by impacts. [1] Zuber et al. 2013 [2] Wieczorek et al. 2013 [3] Han et al. 2013 [4] Besserer et al. 2014 [5] Hurwitz and Kring 2014 [6] Vaughan and Head 2013 [7] Petro and Pieters 2004 [8] Potter et al. 2012

  13. Recent Mission Datasets Shed New Light on the Character and Fate of the South Pole-Aitken Basin Impact Melt Sheet

    NASA Astrophysics Data System (ADS)

    Jolliff, B. L.; Petro, N. E.; Shearer, C. K.; Pieters, C. M.; Head, J. W.

    2016-05-01

    Characterizing and accessing impact melt rocks of the South Pole-Aitken basin is of high priority for understanding the history of the Moon, the giant basin forming process, and establishing the chronology of giant impacts in the early solar system.

  14. Light Plains in the South-Pole Aitken Basin: Surface Ages and Mineralogical Composition

    NASA Astrophysics Data System (ADS)

    Thiessen, F.; Hiesinger, H.; van der Bogert, C. H.; Pasckert, J. H.; Robinson, M. S.

    2012-04-01

    We studied light plains in the north-eastern South-Pole Aitken basin to investigate their origin, ages, and mineralogical composition. Light plains, also known as the Cayley Formation, occur on the near- and farside of the Moon. Due to their smooth texture, lower crater densities, and occurrence as crater fills, they were thought to be of volcanic origin [e.g., 1]. However, Apollo 16 samples of light plains deposits were in fact highly brecciated rocks [2]. Therefore, the Imbrium and Orientale impacts were thought to have formed light plains because they reshaped the surface thousands of kilometers from their impact sites. Subsequent studies revealed varying surface ages of light plains [e.g., 3] and different mineralogical compositions, which are in some cases more highland-like and in others more mare-like. Hence, an origin solely from the Imbrium and/or Orientale impacts is unlikely. Thus, the question whether light plains formed due to large impacts or regional cratering, or through endogenic processes remains open. We performed crater size-frequency measurements [e.g., 4] on Lunar Reconnaissance Orbiter Wide Angle Camera images and obtained absolute model ages between 3.43 and 3.81 Ga. We observed neither a distinctive peak of light plains ages nor clustering of similar ages in any specific regions of the studied area. Due to the fact that the derived ages vary as much as 380 Ma, an origin by a single event seems unlikely. Moreover, some ages even post-date the Imbrium and Orientale impacts, and thus an origin related to those impacts is not likely. Examination of multispectral data from Clementine [5] shows that the Ti abundances vary between 0.2 and 3 wt % and Fe abundances between 12.5 and 19 wt %. We observed a regional difference in distribution: light plains units within the Apollo basin have lower Fe and Ti values and are more highland-like, whereas light plains outside the Apollo basin show higher Fe and Ti values and are more mare-like. Furthermore, M

  15. High Resolution Mapping of the Lunar South Pole-Aitken Basin Interior

    NASA Astrophysics Data System (ADS)

    Archinal, B. A.; Gaddis, L. R.; Hare, T. M.; Rosiek, M.; Howington-Kraus, E.; Lee, E.; Weller, L.; Kirk, R. L.; Edmundson, K.; Becker, T.; Jolliff, B. L.; Tran, T.; Robinson, M.; LROC Science Team

    2010-12-01

    We are making geodetically controlled high-resolution digital terrain models (DTMs) and image orthomosaics of the Constellation (Cx) Program region of interest (ROI) in the lunar South Pole-Aitken Basin interior (“SAB”) (center at 200.06° E, 60.00° S). This work is part of the Lunar Mapping and Modeling Program (LMMP), a NASA-funded effort to create useful cartographic products from past and current lunar datasets and to serve them on a web portal. The SAB is one of 50 ROIs chosen by Cx as potential sites for future robotic or human landings or analogs thereof. Source data for our products includes publically released ~50 cm/pixel Lunar Reconnaissance Orbiter Camera (LROC) Narrow Angle Camera images and Lunar Orbiter Laser Altimeter (LOLA) spot elevation measurements and preliminary global 16 posts/° DTM. Products generated so far include: a) a preliminary stereo DTM covering ~25% of the center 20 km square area of the ROI, with post spacing (resolution) of 1.5 m and ~86x10^6 posts; b) a preliminary mosaic of 90% of the 40 km square ROI, with a resolution of 2 m, orthorectified with LOLA data or, where possible, the stereo DTM; c) preliminary DTMs generated via the use of photoclinometry (“shape from shading”), covering small areas with post spacing of 50 cm; and d) slope and roughness maps derived from a and c. All products are in the lunar mean Earth/polar axis coordinate system and the preliminary global reference frame of the current LOLA DTM. The absolute accuracy of these products is limited by the horizontal and vertical accuracy of the LOLA DTM, to which they are tied (DTMs and mosaics) or on which they are projected (mosaics). The expected vertical precision of the stereo DTM is ~20 cm. The products will soon be publically available via the LMMP portal and also via the USGS planetary GIS site, http://webgis.wr.usgs.gov/. Final versions tied to the LOLA nominal mission global DTM will be available in 2011 October. Preliminary analyses of these

  16. Estimating the Contribution of Basins and Large Craters to the Regolith of the South Pole-Aitken Basin

    NASA Astrophysics Data System (ADS)

    Petro, N. E.; Cohen, B. A.; Jolliff, B. L.; Moriarty, D. P.

    2016-11-01

    Here we revisit the question of how much non-SPA material may have been introduced to SPA. Data from recent lunar missions provides new insight into the composition and geologic evolution of the Moon. We assess the resurfacing effects of large impacts.

  17. Orion/MoonRise: A proposed human & robotic sample return mission from the Lunar South Pole-Aitken Basin

    NASA Astrophysics Data System (ADS)

    Alkalai, L.; Solish, B.; Elliott, J.; McElrath, T.; Mueller, J.; Parker, J.

    This paper describes a new mission concept called Orion/MoonRise that proposes to return samples from the Lunar far-side South Pole-Aitken Basin (SPAB) using a combination of a robotic Sample Return Vehicle (SRV) based on the MoonRise mission concept developed at National Aeronautics and Space Administration's (NASA) Jet Propulsion Laboratory, and the Orion Multi-Purpose Crew Vehicle currently under development by NASA at Lockheed Martin. The mission concept proposes significant challenges for both robotic and human parts of the mission. Whereas there are many ways to execute this mission concept, one approach is for the Orion and the SRV to launch separately. We assume that the Orion will be staged at the Earth-Moon Lagrange Point 2 (EM-L2) and the SRV at EM-L1. Once both are in place, the SRV descends to the SPAB while the Orion provides critical relay coverage with ground control on Earth. During surface operations, the Orion crew tele-operate the lander sampling system and possibly deploy a sample fetch rover. Once the samples are collected, the Lunar Ascent Vehicle (LAV) launches towards the EM-L2 to rendezvous with Orion. The samples are then brought back to Earth for detailed sample curation and analysis by the scientific community. The Orion/MoonRise mission concept has many strengths worth noting: it provides a very exciting mission to be performed in cis-Lunar space, as a precursor to future human exploration beyond the Earth-Moon System and as a technology demonstration for future sample return from Mars; it implements a mission that is of tremendous value to the planetary science community; it provides an exciting and challenging mission for astronauts to perform and demonstrate in deep-space including remote teleoperations and sample rendezvous and capture; and finally it provides an exciting opportunity for the broad engagement of the general public.

  18. Lunar impact basins: New data for the western limb and far side (Orientale and South Pole-Aitken basins) from the first Galileo flyby

    NASA Astrophysics Data System (ADS)

    Head, James W.; Murchie, Scott; Mustard, John F.; Pieters, Carle M.; Neukum, Gerhard; McEwen, Alfred; Greeley, Ronald; Nagel, Engelbert; Belton, Michael J. S.

    1993-09-01

    Compositional aspects of impact basin materials can be analyzed using multispectral image data acquired by the Galileo solid state imaging (SSI) experiment during the December 1990 lunar encounter. These data provide important information on the spectral properties of the western lunar limb and parts of the far side. The SSI images cover the wavelength range 0.4-1.0 μm, allowing measurement of spectral slope and estimation of the strength of the 1 μm absorption due to iron in the mafic minerals olivine and pyroxene. Among deposits of the 930-km-diameter Orientale basin, exterior ejecta comprising the Hevelius Formation is relatively homogeneous and spectrally similar to mature Apollo 16 soils, suggesting an upper crustal source. The centrally located Maunder Formation is distinct from the younger mare basalts but comparable to the Hevelius Formation in its spectral reflectance properties, supporting an interpretation as basin impact melt. The Montes Rook Formation, located in an annulus between the Maunder and the Hevelius, shows a slightly stronger mafic absorption and may be the deepest crustal material excavated. The distal Orientale deposits show local mafic enhancements (in the Schiller-Schickard and Mendel-Rydberg regions) interpreted to represent pre-Orientale mare deposits, or cryptomaria, intermixed with overlying basin ejecta. In this case, maria of sizes comparable to those presently observed were widespread in this region before the Orientale impact. Mixing-model analyses are consistent with the ballistic erosion and sedimentation model for ejecta emplacement in the distal regions beyond the continuous ejecta deposit. On the southern lunar farside, a high area with an enhanced mafic absorption corresponds to the interior and rim of the pre-Nectarian South Pole-Aitken impact basin, 2000-2500 km in diameter. The anomaly is interpreted to be due to several factors, including excavation into the more mafic lower crust, and the presence of extensive early

  19. South Pole-Aitken Sample Return Mission: Collecting Mare Basalts from the Far Side of the Moon

    NASA Technical Reports Server (NTRS)

    Gillis, J. J.; Jolliff, B. L.; Lucey, P. G.

    2003-01-01

    We consider the probability that a sample mission to a site within the South Pole-Aitken Basin (SPA) would return basaltic material. A sample mission to the SPA would be the first opportunity to sample basalts from the far side of the Moon. The near side basalts are more abundant in terms of volume and area than their far-side counterparts (16:1), and the basalt deposits within SPA represent approx. 28% of the total basalt surface area on the far side. Sampling far-side basalts is of particular importance because as partial melts of the mantle, they could have derived from a mantle that is mineralogically and chemically different than determined for the nearside, as would be expected if the magma ocean solidified earlier on the far side. For example, evidence to support the existence of high-Th basalts like those that appear to be common on the nearside in the Procellarum KREEP Terrane has been found. Although SPA is the deepest basin on the Moon, it is not extensively filled with mare basalt, as might be expected if similar amounts of partial melting occurred in the mantle below SPA as for basins on the near side. These observations may mean that mantle beneath the far-side crust is lower in Th and other heat producing elements than the nearside. One proposed location for a sample-return landing site is 60 S, 160 W. This site was suggested to maximize the science return with respect to sampling crustal material and SPA impact melt, however, basaltic samples would undoubtedly occur there. On the basis of Apollo samples, we should expect that basaltic materials would be found in the vicinity of any landing site within SPA, even if located away from mare deposits. For example, the Apollo 16 mission landed in an ancient highlands region 250-300 km away from the nearest mare-highlands boundary yet it still contains a small component of basaltic samples (20 lithic fragments ranging is size from <1 to .01 cm). A soil sample from the floor of SPA will likely contain an

  20. The presence of sboA and spaS genes and antimicrobial peptides subtilosin A and subtilin among Bacillus strains of the Amazon basin.

    PubMed

    Velho, Renata Voltolini; Basso, Ana Paula; Segalin, Jeferson; Costa-Medina, Luis Fernando; Brandelli, Adriano

    2013-03-01

    This report demonstrates the usefulness of PCR for the genes spaS and sboA as a means of identifying Bacillus strains with a potential to produce subtilin and subtilosin A. One collection strain and five Bacillus spp. isolated from aquatic environments in the Amazon basin were screened by PCR using primers for sboA and spaS designed specifically for this study. The sequences of the PCR products showed elevated homology with previously described spaS and sboA genes. Antimicrobial peptides were isolated from culture supernatants and analyzed by mass spectrometry. For all samples, the mass spectra revealed clusters with peaks at m/z 3300-3500 Da, corresponding to subtilosin A, subtilin and isoforms of these peptides. These results suggest that the antimicrobial activity of these strains may be associated with the production of subtilosin A and/or subtilin. The PCR used here was efficient in identifying novel Bacillus strains with the essential genes for producing subtilosin A and subtilin.

  1. Well known outstanding geoid and relief depressions as regular wave woven features on Eartg (Indian geoid minimum), Moon (SPA basin), Phobos (Stickney crater), and Miranda (an ovoid).

    NASA Astrophysics Data System (ADS)

    Kochemasov, Gennady G.

    2010-05-01

    A very unreliable interpretation of the deepest and large depressions on the Moon and Phobos as the impact features is not synonymous and causes many questions. A real scientific understanding of their origin should take into consideration a fact of their similar tectonic position with that of a comparable depression on so different by size, composition, and density heavenly body as Earth. On Earth as on other celestial bodies there is a fundamental division on two segments - hemispheres produced by an interference of standing warping wave 1 (long 2πR) of four directions [1]. One hemisphere is uplifted (continental, highlands) and the opposite subsided (oceanic, lowlands). Tectonic features made by wave 2 (sectors) adorn this fundamental structure. Thus, on the continental risen segment appear regularly disposed sectors, also uplifted and subsided. On the Earth's eastern continental hemisphere they are grouped around the Pamirs-Hindukush vertex of the structural octahedron made by interfering waves2. Two risen sectors (highly uplifted African and the opposite uplifted Asian) are separated by two fallen sectors (subsided Eurasian and the opposite deeply subsided Indoceanic). The Indoceanic sector with superposed on it subsided Indian tectonic granule (πR/4-structure) produce the deepest geoid minimum of Earth (-112 m). The Moon demonstrates its own geoid minimum of the same relative size and in the similar sectoral tectonic position - the SPA basin [2, 3]. This basin represents a deeply subsided sector of the sectoral structure around the Mare Orientale (one of vertices of the lunar structural octahedron). To this Mare converge four sectors: two subsided - SPA basin and the opposite Procellarum Ocean, and two uplifted - we call them the "Africanda sector" and the opposite "Antiafricanda one" to stress structural similarity with Earth [2]. The highest "Africanda sector" is built with light anorthosites; enrichment with Na makes them even less dense that is required

  2. Viscoelastic Relaxation of Lunar Basins

    NASA Astrophysics Data System (ADS)

    Mohit, P. S.; Phillips, R. J.

    2004-12-01

    The large lunar impact basins provide a unique glimpse into early lunar history. Here we investigate the possibility that the relief of the oldest lunar basins (with the exception of South-Pole Aitken) has decayed through viscous relaxation. We identify nine ancient multi-ring basins with very low relief and low-amplitude Bouguer and free-air gravity anomalies. The characteristics of these basins are consistent with either 1) relaxation of topographic relief by ductile flow (e.g. Solomon et al., 1982) or 2) obliteration of basin topography during crater collapse immediately following impact. Both scenarios require that the basins formed early in lunar history, when the Moon was hot. The latter possibility appears to be unlikely due to the great topographic relief of South Pole-Aitken basin (SPA), the largest and oldest impact basin on the Moon (with the possible exception of the putative Procellarum basin; Wilhelms, 1987). On the other hand, the thin crust beneath SPA may not have allowed ductile flow in its lower portions, even for a hot Moon, implying that a thicker crust is required beneath other ancient basins for the hypothesis of viscous relaxation to be tenable. Using a semi-analytic, self-gravitating viscoelastic model, we investigate the conditions necessary to produce viscous relaxation of lunar basins. We model topographic relaxation for a crustal thickness of 30 km, using a dry diabase flow law for the crust and dry olivine for the mantle. We find that the minimum temperature at the base of the crust (Tb) permitting nearly complete relaxation of topography by ductile flow on a timescale < 108 yrs is 1400 K, corresponding to a heat flow of 55mW/m2, into the crust. Ductile flow in the lower crust becomes increasingly difficult as the crustal thickness decreases. The crust beneath SPA, thinned by the impact, is only 15-20 km thick and would require Tb ≥ 1550 K for relaxation to occur. The fact that SPA has maintained high-amplitude relief suggests that

  3. Spa in Counseling.

    ERIC Educational Resources Information Center

    Nelson, Richard C.

    1992-01-01

    Explains spa in counseling as uplifting, positive experiences in which focus is placed directly on helping clients feel good about themselves. Provides several examples of spa in counseling, simplest procedure being activity "Things I Can Do," in which counselor helps client focus on and savor some of most often-repeated actions of which he is…

  4. Medical spa marketing.

    PubMed

    Sadick, Neil S; Dinkes, Adam; Oskin, Larry

    2008-07-01

    Medical spas are different. We are not just selling medical and dermatology services; we are offering clients viable new solutions to their skin care, body care, and hair care challenges. Traditional medical marketing becomes blurred today, as the expansion and acceptance of medical spas helps you to effectively compete with traditional skin care clinics, salons, and spas, while offering more therapeutic treatments from professionally licensed doctors, nurses, aestheticians, massage therapists, spa professionals, and medical practitioners. We recommend that you make the choice to successfully and competitively become a market-driven medical spa with an annual strategic plan, rather than an operationally driven business.

  5. Software Process Assessment (SPA)

    NASA Technical Reports Server (NTRS)

    Rosenberg, Linda H.; Sheppard, Sylvia B.; Butler, Scott A.

    1994-01-01

    NASA's environment mirrors the changes taking place in the nation at large, i.e. workers are being asked to do more work with fewer resources. For software developers at NASA's Goddard Space Flight Center (GSFC), the effects of this change are that we must continue to produce quality code that is maintainable and reusable, but we must learn to produce it more efficiently and less expensively. To accomplish this goal, the Data Systems Technology Division (DSTD) at GSFC is trying a variety of both proven and state-of-the-art techniques for software development (e.g., object-oriented design, prototyping, designing for reuse, etc.). In order to evaluate the effectiveness of these techniques, the Software Process Assessment (SPA) program was initiated. SPA was begun under the assumption that the effects of different software development processes, techniques, and tools, on the resulting product must be evaluated in an objective manner in order to assess any benefits that may have accrued. SPA involves the collection and analysis of software product and process data. These data include metrics such as effort, code changes, size, complexity, and code readability. This paper describes the SPA data collection and analysis methodology and presents examples of benefits realized thus far by DSTD's software developers and managers.

  6. An interpretation of volcanic and structural features of crater Aitken. [from Apollo 17 panoramic photography

    NASA Technical Reports Server (NTRS)

    Bryan, W. B.; Adams, M.-L.

    1974-01-01

    Detailed observations from the study of Apollo 17 panoramic photography of the Aitken crater are reported which suggest that there has been significant late-stage compressional deformation of the crater and its adjacent highlands. A speculative interpretation of eruptive activity and drain-back events within Aitken is presented, which leads to the conclusion that hummocky topography within certain cones represents collapsed lava rather than extrusive domes. That is, eruptive activity within Aitken probably commenced with an explosive cone-building stage, followed by lava eruptions from cones and fissures, and ended with drain-back restricted to the relatively deep lava ponded in the vents.

  7. Robert G. Aitken and His ADS: Double Star Oberver, Cataloguer, Statistician, and Observatory Director

    NASA Astrophysics Data System (ADS)

    Osterbrock, D. E.

    2000-05-01

    Robert G. Aitken was a dynamical astronomer of the old school, a long-time visual double star observer. He was born in 1864 in Jackson, California, a small town in the Gold Country midway between Yosemite and Sacramento. His education at Williams College under Truman Safford; his early teaching career at Livermore College and the University of the Pacific; his simultaneous graduate reading course in mathematics; and his becoming a professional astronomer under the tutelage of Edward S. Holden and Edward E. Barnard at Lick Observatory will be described. Aitken made a systematic survey of the entire sky north of -30 degrees for double stars, joined by William J. Hussey for a time. It produced important new information on binary and multiple stars and their orbits. His book The Binary Stars and his New General Catalogue of Double Stars (ADS) were his monuments. Aitken was associate director of Lick Observatory from 1923 until 1930, while W. W. Campbell was simultaneously director and president of the University of California. Then Aitken was director himself from 1930 until he retired in 1935 and moved to Berkeley, where he continued writing until his death in 1951. Aitken was editor of the PASP for 51 years. He hoped that Gerard P. Kuiper would succeed him as the double star observer at Lick Observatory, but that was not to be. Aitken at various times held every office in the ASP, and was vice president, then president, of the AAS.

  8. The Health Spa Industry and the Profession.

    ERIC Educational Resources Information Center

    Swengros, Glenn V.

    During the past three decades, the health spa industry has grown to become a viable component of our society. Many people, however, still have reservations about the sincerity of health spa proprietors. This is a result of nonprofessional management in the first years of business. Today the health spa industry finds itself with changed…

  9. Measurements of radon concentrations in Spa waters in Amasya, Turkey

    NASA Astrophysics Data System (ADS)

    Yigitoglu, I.; Oner, F.; Yalim, H. A.; Ucar, B.

    2016-04-01

    The aim of this study is to determine the radon concentrations in thermal waters in the Amasya basin in Turkey and to explore the relationship between radon anomalies and active geological faults. The radon concentration measurements were performed in four thermal Spas around Amasya basin. The water samples were collected from tap waters in thermal water sources. The obtained radon concentrations ranged from 0.15 ± 0.12 to 0.71 ± 0.32 BqL-1 for Spa waters. The relationship between the radon concentration anomalies and earthquakes that occurred in the sampling period are discussed.

  10. Yeasts in spa establishments.

    PubMed

    Svorcová, L

    1982-05-01

    It was investigated occurrence of yeasts on bathsurfaces, in sauna rooms, in swimming and therapeutic pool water. The number of yeasts decreased depending on patients age, if the rooms were furnished with bath. The lowest contamination was found after bath of 40-60 years-old women. In the saunas were yeasts not found on the upper benches with temperature above 55 degrees C. Much higher counts on lower benches and wood mats with temperature 35-40 degrees C, on basin walls and bottom-up to 10(4)-10(6)/100 cm2. It was isolated 172 yeast strains. The occurrence of some selected strains is given in Table 7, with the toxic effect of disinfectants. The most strains were resistant to Peracetic acid and Chloramin B. Since most of the isolated and determinated strains were found in contaminated environment or during various diseases, the yeasts of the genus Cryptococcus, Candida, Rhodotorula, Torulopsis and Metschnikowia should not occur in bath establishment, and should be classified among indicators of contamination of environment including water. PMID:7124167

  11. The SPaCIFY Project

    NASA Astrophysics Data System (ADS)

    Chemouil, D.

    2008-08-01

    SPaCIFY is a research project aiming at developing a design environment for spacecraft flight software. More precisely, the project shall promote a top-down method based upon multi-clock synchronous modelling, formally - verified transformations, exhaustive verification through model-checking and a runtime framework featuring realtime - friendly distribution and dynamic-reconfiguration services. Furthermore, the various tools shall be released under FLOSS (free/libre/open-source software) licenses, favouring cost-sharing and sustainability.

  12. Condensation-nuclei (Aitken Particle) measurement system used in NASA global atmospheric sampling program

    NASA Technical Reports Server (NTRS)

    Nyland, T. W.

    1979-01-01

    The condensation-nuclei (Aitken particle) measuring system used in the NASA Global Atmospheric Sampling Program is described. Included in the paper is a description of the condensation-nuclei monitor sensor, the pressurization system, and the Pollack-counter calibration system used to support the CN measurement. The monitor has a measurement range to 1000 CN/cm cubed and a noise level equivalent to 5 CN/cm cubed at flight altitudes between 6 and 13 km.

  13. [Nonnius and the Spa cure].

    PubMed

    Lecomte, J; Lemli, J; Marganne, M H; Melard, M

    1996-01-01

    The 12th of July 1635, Jan van Beverwyck wrote a letter from Dordrecht to his esteemed friend Luis Nunez, in Antwerp. He asked him for indications about the cure of kidney stones of which he was suffering. He had just passed two calculi very painfully. Nunez answered on July 31. His letter summarized his own experience with this disease. In such cases, temperate diuretics and emollients are well indicated: ingestion of light decoctions of Malva and Viola, Caerefolium and Parieteria as well as Marshmallow syrup. No purge and no 'chemicals'. However, the best remedy was drinking Spa water. Usually the best way to absorb it is to come to the springs, in the Ardennes, part of the independent Principality of Liège. However, considering the horrors of the war (the Thirty Years War) endangering the country, van Beverwyck would be wise to drink imported water during 40 or 50 days, while staying home quietly, in Dordrecht. The letter points out the importance of provoked diuresis in the treatment of kidney stones. On the other hand the letter is an illustration of the continuing contacts between the United Provinces and the Spanish territories and of the commercial traffic between Antwerp, Dordrecht and Spa. The exported water was bottled from many 'pouhons'. It was acid, ferruginous, sulfurous and saturated with carbon dioxide; no pathogen germs, at least at the spring. Nunez quoted the large number of his patients cured with Spa water in Antwerp. This is an illustration of its commercial expansion even during this critical period. PMID:8848877

  14. Orientale and South Pole-Aitken basins on the Moon: Preliminary Galileo imaging results

    NASA Technical Reports Server (NTRS)

    Head, J.; Fischer, E.; Murchie, S.; Pieters, C.; Plutchak, J.; Sunshine, J.; Belton, M.; Carr, M.; Chapman, C.; Davies, M.

    1991-01-01

    During the Earth-Moon flyby the Galileo Solid State Imaging System obtained new information on the landscape and physical geology of the Moon. Multicolor Galileo images of the Moon reveal variations in color properties of the lunar surface. Using returned lunar samples as a key, the color differences can be interpreted in terms of variations in the mineral makeup of the lunar rocks and soil. The combined results of Apollo landings and multicolor images from Galileo allow extrapolation of surface composition to areas distant from the landing sites, including the far side invisible from Earth.

  15. 33 CFR 117.571 - Spa Creek.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Spa Creek. 117.571 Section 117.571 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Maryland § 117.571 Spa Creek. The S181 bridge, mile 4.0,...

  16. Ozone and Aitken nuclei over equatorial Africa - Airborne observations during DECAFE 88

    NASA Technical Reports Server (NTRS)

    Andreae, M. O.; Chapuis, A.; Cros, B.; Fontan, J.; Helas, G.; Justice, C.; Kaufman, Y. J.; Minga, A.; Nganga, D.

    1992-01-01

    Results of ozone and Aitken condensation nuclei measurements made over the rain forest in equatorial Africa during February 12-25, 1988 are presented. The results indicate the presence of a layer between 1 and 4 km altitude where these species are strongly enriched. Based on information derived from simultaneous measurements of other chemical and meteorological parameters, satellite imagery, and trajectory calculations, this enrichment is attributed to emissions from biomass burning in sub-Saharan Africa, from which ozone is formed by photochemical reactions.

  17. From sulphur to perfume: spa and SPA at Monchique, Algarve.

    PubMed

    Bastos, Cristiana

    2011-04-01

    In the thermal village of Monchique, Algarve, different streams of water-related knowledge and practices coexisted for centuries. Those waters were traditionally known as aguas santas (holy waters) and believed to have redemptive healing powers. In the seventeenth century, the Catholic church took control of the place, refashioned the bathing rituals, developed infrastructures and provided assistance to the patients, granting free treatment to the poor. In the nineteenth century, the state replaced the church and imposed that treatments should be provided by professionals trained in the scientific principles of medical hydrology. Secular and scientific as they were, clinical logbooks still allowed for the account of patients that embodied miracle-like redemptive cures 'at the third bath'. People went to Monchique both for its magic and its medicine, bringing in the body ailments achieved in their lives of hard labour. They also went there for a socialising break while healing. From mendicants to rich landowners, coming mostly from the Algarve and neighbouring Alentejo, they crowded the place in summertime. In the twentieth century, as in other places in continental Europe, the spa evolved into a highly medicalised place that qualified for medical expenses reimbursements, which implied the eclipsing--at least from representation--of its leisure component. In the twenty-first century, a new trend of consumer-centred, market-based, post-water balneology with an emphasis on wellness and leisure reinvented the spa as place for lush and diversified consumption. This article argues that the seemingly contradictory systems (markets and medicine) coexist much in the same way that magic, religion and medicine coexisted in the old water sites. The new SPAs, rather than putting an end to the old spas, have enabled them to survive by reinventing thermal sites as places of attraction and leisure.

  18. Geologic Mapping of the Lunar South Pole, Quadrangle LQ-30: Volcanic History and Stratigraphy of Schroedinger Basin

    NASA Technical Reports Server (NTRS)

    Mest, S. C.; Berman, D. C.; Petro, N. E.

    2009-01-01

    In this study we use recent images and topographic data to map the geology and geomorphology of the lunar South Pole quadrangle (LQ-30) at 1:2.5M scale [1-4] in accordance with the Lunar Geologic Mapping Program. Mapping of LQ-30 began during Mest's postdoctoral appointment and has continued under the PG&G Program, from which funding became available in February 2009. Preliminary map-ping and analyses have been done using base materials compiled by Mest, but properly mosaicked and spatially registered base materials are being compiled by the USGS and should be received by the end of June 2009. The overall objective of this research is to constrain the geologic evolution of the lunar South Pole (LQ-30: 60deg -90deg S, 0deg - +/-180deg ) with specific emphasis on evaluation of a) the regional effects of basin formation on the structure and composition of the crust and b) the spatial distribution of ejecta, in particular resulting from formation of the South Pole-Aitken (SPA) basin and other large basins. Key scientific objectives include: 1) Constraining the geologic history of the lunar South Pole and examining the spatial and temporal variability of geologic processes within the map area. 2) Constraining the vertical and lateral structure of the lunar regolith and crust, assessing the distribution of impact-generated materials, and determining the timing and effects of major basin-forming impacts on crustal structure and stratigraphy in the map area. And 3) assessing the distribution of resources (e.g., H, Fe, Th) and their relationships with surface materials.

  19. Geology and petrology of enormous volumes of impact melt on the Moon: A case study of the Orientale basin impact melt sea

    NASA Astrophysics Data System (ADS)

    Vaughan, William M.; Head, James W.; Wilson, Lionel; Hess, Paul C.

    2013-04-01

    Lunar basin-forming impacts produce enormous volumes (>105 km3) of impact melt. All known basin-forming impacts combined may produce ˜108 km3 of impact melt, ˜1/20th the volume of the lunar crust. Despite their volumetric importance, the geology and petrology of massive deposits of impact melt on the Moon have been little studied, in part because most basin impact melt deposits are old and have been obscured or buried by subsequent impact cratering and mare infill. We investigate the geology and model the petrology of fresh massive impact melt deposits in the relatively young 930 km diameter Orientale basin. Models of impact melt production combined with geologic analyses based on new LOLA topographic data suggest that most of the impact melt (˜2/3) produced by the Orientale-forming impact occurs in a ˜15 km thick impact melt sheet (better described as an impact melt sea) ˜350 km in diameter with a volume of ˜106 km3. We anticipate that the Orientale melt sea has undergone large-scale igneous differentiation, since terrestrial impact melt sheets (such as Manicouagan, Sudbury, and Morokweng) less than a tenth of the thickness and a hundredth of the volume of the Orientale melt sea have differentiated. We develop a model for the cumulate stratigraphy of the solidified Orientale impact melt sea. A modeled cumulate stratigraphy (occurring below a quench crust and anorthositic fallback breccia) with an ˜8 km thick layer of norite overlying a ˜4 km layer of pyroxenite and a basal ˜2 km thick layer of dunite produced by equilibrium crystallization of a homogenized melt sea, consistent with vigorous convection in that melt sea, is supported by remotely-sensed norite excavated by the central peak of Maunder crater from ˜4 km depth. Generally, we predict that very large basin-forming impacts, including the South Pole-Aitken (SPA) basin-forming impact, produce melt seas with a cumulate stratigraphy similar to that of the Orientale melt sea. Impact melt

  20. SPA Meteor Section Results: 2007

    NASA Astrophysics Data System (ADS)

    McBeath, Alastair

    2013-08-01

    Information extracted from analyses carried out by the SPA Meteor Section from 2007 is presented and discussed. Events covered include: the radio Quadrantid maximum on January 4; a bright fireball seen from parts of England and imaged from the Netherlands at 19h56m UT on February 6, for which an approximate trajectory was established; radio results from the Lyrids in late April; the Perseid near-peak activity from August and a note on some daylight Perseid observing from Britain using thermal imagers; the radio α-Aurigid maximum on September 1; the Orionid return, which again provided enhanced activity over several consecutive dates in October for visual and radio observers; the radio Leonids, although the probably main peak found visually on November 19 was not recorded thus due to its timing; the typically protracted Geminid maximum period around December 13-15 as observed visually and by radio; and the Ursid outburst, primarily as detected by radio on December 22.

  1. An Identification Method of Magnetizing Inrush Current Phenomena in Distribution System using with Aitken's Δ2-process

    NASA Astrophysics Data System (ADS)

    Ozeki, Takayuki; Naitoh, Tadashi; Toyama, Atsushi; Satoh, Kohki; Takeda, Keiki; Ohta, Fumihiko

    In this paper, the authors propose an improved identification method of the magnetizing inrush current phenomena. To improve the estimation of the saturation on/off time, the Aitken's Δ2-process is adopted. And a new index δI, which is a variation current, is introduced. The merits of Aitken's Δ2-process are the simple algorithm and the high precision to oscillation noise. Then, the proposed method is adapted to a measured data, in which the oscillation noise is caused. The proposed method gives a high precision in these cases.

  2. Incorporating a medical spa into a physician-run practice.

    PubMed

    Katz, Bruce; McBean, Jason

    2008-07-01

    The age-old spa concept is no less valuable today than it was during the time of Julius Caesar. Over the centuries, there have been many iterations of the concept of the spa. The most recent is the medical spa, which has become the fastest-growing segment of the spa industry. Many physicians including dermatologists wish to incorporate a medical spa into their practices. This article discusses the key elements that should be considered to make this venture successful. PMID:18555948

  3. 33 CFR 117.571 - Spa Creek.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... OPERATION REGULATIONS Specific Requirements Maryland § 117.571 Spa Creek. The S181 bridge, mile 0.4, at Annapolis, Maryland: (a) From May 1 to October 31, Monday through Friday, except Federal and State...

  4. 33 CFR 117.571 - Spa Creek.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... OPERATION REGULATIONS Specific Requirements Maryland § 117.571 Spa Creek. The S181 bridge, mile 4.0, at Annapolis, Maryland: (a) From May 1 to October 31, Monday through Friday, except Federal and State...

  5. 33 CFR 117.571 - Spa Creek.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... OPERATION REGULATIONS Specific Requirements Maryland § 117.571 Spa Creek. The S181 bridge, mile 0.4, at Annapolis, Maryland: (a) From May 1 to October 31, Monday through Friday, except Federal and State...

  6. 33 CFR 117.571 - Spa Creek.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... OPERATION REGULATIONS Specific Requirements Maryland § 117.571 Spa Creek. The S181 bridge, mile 0.4, at Annapolis, Maryland: (a) From May 1 to October 31, Monday through Friday, except Federal and State...

  7. Health tourism in a Czech health spa.

    PubMed

    Speier, Amy R

    2011-04-01

    This paper is about the changing shape of health tourism in a Czech spa town. The research focuses on balneotherapy as a traditional Czech healing technique, which involves complex drinking and bathing therapies, as it is increasingly being incorporated into the development of a Czech health tourism industry. Today, the health tourism industry in Mariánske Lázne is attempting to 'harmoniously' combine three elements--balneology, travel and business activities. One detects subtle shifts and consequent incongruities as doctors struggle for control over the medical portion of spa hotels. At the same time, marketing groups are creating new packages for a general clientele, and the implementation of these new packages de-medicalizes balneotherapy. Related to the issue of the doctor's authority in the spa, the changes occurring with the privatization of tourism entails the entrance of 'tourists' to Mariánske Lázne who are not necessarily seeking spa treatment but who are still staying at spa hotels. There is a general consensus among spa doctors and employees that balneotherapy has become commodified. Thus, while balneotherapy remains a traditional form of therapy, the commercial context in which it exists has created a new form of health tourism.

  8. SPA Meteor Section Results: 2006

    NASA Astrophysics Data System (ADS)

    McBeath, Alastair

    2010-12-01

    A summary of the main analyzed results and other information provided to the SPA Meteor Section from 2006 is presented and discussed. Events covered include: the radio Quadrantid maximum on January 3/4; an impressive fireball seen from parts of England, Belgium and the Netherlands at 22h53m51s UT on July 18, which was imaged from three EFN stations as well; the Southern delta-Aquarid and alpha-Capricornid activity from late July and early August; the radio Perseid maxima on August 12/13; confirmation that the October 5/6 video-meteor outburst was not observed by radio; visual and radio findings from the strong, bright-meteor, Orionid return in October; another impressive UK-observed fireball on November 1/2, with an oil painting of the event as seen from London; the Leonids, which produced a strong visual maximum around 04h-05h UT on November 18/19 that was recorded much less clearly by radio; radio and visual reports from the Geminids, with a note regarding NASA-observed Geminid lunar impact flashes; and the Ursid outburst recorded by various techniques on December 22.

  9. [Kanjiza Spa: ninety years of healing].

    PubMed

    Jokanović, Vladimir

    2004-01-01

    "Kanjiza" spa was founded in 1903. It was an artesian bath at the beginning, but gradually it has grown into a modern rehabilitation center. Much of its existence the spa owes to Herman Gruenfeld, a local businessman who was the first one to invest in development of natural resources in medical purposes. In this work the author reviews some earlier analyses of the chemical composition of water in Kanjiza spa. Procedures are illustrated as well as laboratory findings in Budapest and Belgrade. The author brings to attention the current condition of certain parts of the Spa, which are supposed to be under governmental care and protection. Many of the older building are in bad condition. Trees are damaged in the park, and little has been renewed lately. This paper emphasizes the diversity and efficiency of medical procedures performed by 150 members of the team lead by 10 doctors, and many problems and hardships they successfully face in the health system of Serbia. Due to unpredicted circumstances the centennial anniversary of Kanjiza spa was not marked depriving the public of rare opportunity to recognize efforts and accomplishments of this many aspects unique institution belonging to the very top medical centers specialized in rehabilitation in our country.

  10. Supersonic Wing Optimization Using SpaRibs

    NASA Technical Reports Server (NTRS)

    Locatelli, David; Mulani, Sameer B.; Liu, Qiang; Tamijani, Ali Y.; Kapania, Rakesh K.

    2014-01-01

    This research investigates the advantages of using curvilinear spars and ribs, termed SpaRibs, to design a supersonic aircraft wing-box in comparison to the use of classic design concepts that employ straight spars and ribs. The objective is to achieve a more efficient load-bearing mechanism and to passively control the deformation of the structure under the flight loads. Moreover, the use of SpaRibs broadens the design space and allows for natural frequencies and natural mode shape tailoring. The SpaRibs concept is implemented in a new optimization MATLAB-based framework referred to as EBF3SSWingOpt. This optimization scheme performs both the sizing and the shaping of the internal structural elements, connecting the optimizer with the analysis software. The shape of the SpaRibs is parametrically defined using the so called Linked Shape method. Each set of SpaRibs is placed in a one by one square domain of the natural space. The set of curves is subsequently transformed in the physical space for creating the wing structure geometry layout. The shape of each curve of each set is unique; however, mathematical relations link the curvature in an effort to reduce the number of design variables. The internal structure of a High Speed Commercial Transport aircraft concept developed by Boeing is optimized subjected to stress, subsonic flutter and supersonic flutter constraints. The results show that the use of the SpaRibs allows for the reduction of the aircraft's primary structure weight without violating the constraints. A weight reduction of about 15 percent is observed.

  11. spa typing for epidemiological surveillance of Staphylococcus aureus.

    PubMed

    Hallin, Marie; Friedrich, Alexander W; Struelens, Marc J

    2009-01-01

    The spa typing method is based on sequencing of the polymorphic X region of the protein A gene (spa), present in all strains of Staphylococcus aureus. The X region is constituted of a variable number of 24-bp repeats flanked by well-conserved regions. This single-locus sequence-based typing method combines a number of technical advantages, such as rapidity, reproducibility, and portability. Moreover, due to its repeat structure, the spa locus simultaneously indexes micro- and macrovariations, enabling the use of spa typing in both local and global epidemiological studies. These studies are facilitated by the establishment of standardized spa type nomenclature and Internet shared databases.

  12. An overview of pool and spa regulations in Mediterranean countries with a focus on the tourist industry.

    PubMed

    Mavridou, Athena; Pappa, Olga; Papatzitze, Olga; Blougoura, Athena; Drossos, Panagiotis

    2014-09-01

    The Mediterranean area is densely populated and a very popular tourist destination. This study aimed at gathering up-to-date information on current national pool and spa regulations. We formulated and duly distributed to 20 Mediterranean countries a questionnaire divided into two sections: for pool and spa facilities, respectively. The questionnaire was formulated in such a way that a positive answer would imply that the specific topic was addressed effectively. While all 20 countries responded to the swimming-pool-related questionnaire, only 11 of these countries reported the existence of spa-related regulations. When combining the response overall of all countries for pool and spa facilities together, of a grand total of 606 answers, 183 (30.2%) were positive. The positive answers in the pool section of the questionnaire were 29% of the total of 420 answers while positive answers in the spa section of the questionnaire were fewer (27.8% of the total of 176 answers). The countries were grouped by geographical area of the Mediterranean basin where they are situated. Also, the questions were grouped into broad thematic categories. The paper presents conclusions drawn on the basis of the data received according to these geographical and thematic groupings.

  13. SPA Meteor Section results: September - October, 1995.

    NASA Astrophysics Data System (ADS)

    McBeath, A.

    1996-04-01

    A compilation of visual, photographic and radio results submitted to the SPA Meteor Section from September and October is given. Although September was not an especially good month for observers, October brought a quite well-viewed Orionid return, enabling some magnitude and train details to be derived for the shower, and another brilliant fireball occurred at 2h28mUT on October 31.

  14. Supersymmetry Parameter Analysis: SPA Convention and Project

    SciTech Connect

    Hinchliffe, I.; et al.

    2005-05-05

    High-precision analyses of supersymmetry parameters aim atreconstructing the fundamental supersymmetric theory and its breakingmechanism. A well defined theoretical framework is needed whenhigher-order corrections are included. We propose such a scheme,Supersymmetry Parameter Analysis SPA, based on a consistent set ofconventions and input parameters. A repository for computer programs isprovided which connect parameters in different schemes and relate theLagrangian parameters to physical observables at LHC and high energy e+e-linear collider experiments, i.e., masses, mixings, decay widths andproduction cross sections for supersymmetric particles. In addition,programs for calculating high-precision low energy observables, thedensity of cold dark matter (CDM) in the universe as well as the crosssections for CDM search experiments are included. The SPA scheme stillrequires extended efforts on both the theoretical and experimental sidebefore data can be evaluated in the future at the level of the desiredprecision. We take here an initial step of testing the SPA scheme byapplying the techniques involved to a specific supersymmetry referencepoint.

  15. Genetic complexity of the human surfactant-associated proteins SP-A1 and SP-A2

    PubMed Central

    Silveyra, Patricia; Floros, Joanna

    2012-01-01

    Pulmonary surfactant protein A (SP-A) plays a key role in innate lung host defense, in surfactant-related functions, and in parturition. In the course of evolution, the genetic complexity of SP-A has increased, particularly in the regulatory regions (i.e. promoter, untranslated regions). Although most species have a single SP-A gene, two genes encode SP-A in humans and primates (SFTPA1and SFTPA2). This may account for the multiple functions attributed to human SP-A, as well as the regulatory complexity of its expression by a relatively diverse set of protein and non-protein cellular factors. The interplay between enhancer cis-acting DNA sequences and trans-acting proteins that recognize these DNA elements is essential for gene regulation, primarily at the transcription initiation level. Furthermore, regulation at the mRNA level is essential to ensure proper physiological levels of SP-A under different conditions. To date, numerous studies have shown significant complexity of the regulation of SP-A expression at different levels, including transcription, splicing, mRNA decay, and translation. A number of trans-acting factors have also been described to play a role in the control of SP-A expression. The aim of this report is to describe the genetic complexity of the SFTPA1 and SFTPA2 genes, as well as to review regulatory mechanisms that control SP-A expression in humans and other animal species. PMID:23069847

  16. Pulmonary surfactant protein A (SP-A) specifically binds dipalmitoylphosphatidylcholine

    SciTech Connect

    Kuroki, Y.; Akino, T. )

    1991-02-15

    Phospholipids are the major components of pulmonary surfactant. Dipalmitoylphosphatidylcholine is believed to be especially essential for the surfactant function of reducing the surface tension at the air-liquid interface. Surfactant protein A (SP-A) with a reduced denatured molecular mass of 26-38 kDa, characterized by a collagen-like structure and N-linked glycosylation, interacts strongly with a mixture of surfactant-like phospholipids. In the present study the direct binding of SP-A to phospholipids on a thin layer chromatogram was visualized using 125I-SP-A as a probe, so that the phospholipid specificities of SP-A binding and the structural requirements of SP-A and phospholipids for the binding could be examined. Although 125I-SP-A bound phosphatidylcholine and sphingomyeline, it was especially strong in binding dipalmitoylphosphatidylcholine, but failed to bind phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, and phosphatidylserine. Labeled SP-A also exhibited strong binding to distearoylphosphatidylcholine, but weak binding to dimyristoyl-, 1-palmitoyl-2-linoleoyl-, and dilinoleoylphosphatidylcholine. Unlabeled SP-A readily competed with labeled SP-A for phospholipid binding. SP-A strongly bound dipalmitoylglycerol produced by phospholipase C treatment of dipalmitoylphosphatidylcholine, but not palmitic acid. This protein also failed to bind lysophosphatidylcholine produced by phospholipase A2 treatment of dipalmitoylphosphatidylcholine. 125I-SP-A shows almost no binding to dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylethanolamine. The addition of 10 mM EGTA into the binding buffer reduced much of the 125I-SP-A binding to phospholipids. Excess deglycosylated SP-A competed with labeled SP-A for binding to dipalmitoylphosphatidylcholine, but the excess collagenase-resistant fragment of SP-A failed.

  17. Trajectory Design for MoonRise: A Proposed Lunar South Pole-Aitken Basin Sample Return Mission

    NASA Technical Reports Server (NTRS)

    Parker, Jeffrey S.; McElrath, Timothy P.; Anderson, Rodney L.; Sweetser, Theodore H.

    2013-01-01

    This paper presents the mission design for the proposed MoonRise New Frontiers mission: a lunar far side lander and return vehicle, with an accompanying communication satellite. Both vehicles are launched together, but fly separate low-energy transfers to the Moon. The communication satellite enters lunar orbit immediately upon arrival at the Moon, whereas the lander enters a staging orbit about the lunar Lagrange points. The lander descends and touches down on the surface 17 days after the communication satellite enters orbit. The lander remains on the surface for nearly two weeks before lifting off and returning to Earth via a low-energy return.

  18. Analysis of landing site attributes for future missions targeting the rim of the lunar South Pole Aitken basin

    NASA Astrophysics Data System (ADS)

    Koebel, David; Bonerba, Michele; Behrenwaldt, Daniel; Wieser, Matthias; Borowy, Carsten

    2012-11-01

    For the South polar lunar region between -85 and -90° Latitude an updated analyses of the solar illumination and ground station visibility conditions has been performed in the frame of a feasibility study for an ESA Lunar Lander mission. The analyses are based on the refined lunar digital elevation model provided by the Japanese Kaguya/Selene mission, originating from its LASER altimeter instrument. For the South polar region maps of integral solar illumination are presented for a mission epoch in 2016. The analysis modelling was validated with the help of a Kaguya High Definition video. The solar illumination is driving for the power subsystems of any robotic lander craft or manned lunar outpost, in case they rely on conventional photovoltaic power generation with battery buffering of shadowed periods. In addition the visibility of the terrain from a terrestrial ESA ground station was analysed. The results are presented as an integral ground contact duration map, being crucial for the operations of any lunar outpost. Considering these two quality criteria, several possible landing sites for a future lunar mission have been pre-selected. For these sites a detailed analysis of quasi-continuous illumination conditions is presented. This includes magnified maps of the pre-selected areas, showing any location's longest illumination intervals that are allowed to be interrupted by shadows with limited duration only. As a final quality criterion, the terrain topology has been analysed for its impact on the landing trajectory. From a trade-off between the three quality criteria the connecting ridge between the Shackleton and the de Gerlache was determined to provide the most favourable landing site quality. This site is located at 89°28' South, 136°40' West, and 1947 m altitude, and features and integral illumination of 85.7%. With battery energy to sustain shadows of 120 h, total mission duration of 9.37 sidereal months can be guaranteed.

  19. 7 CFR 2902.35 - Bathroom and spa cleaners.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Bathroom and spa cleaners. 2902.35 Section 2902.35 Agriculture Regulations of the Department of Agriculture (Continued) OFFICE OF ENERGY POLICY AND NEW USES... Items § 2902.35 Bathroom and spa cleaners. (a) Definition. Products that are designed to clean...

  20. 7 CFR 2902.35 - Bathroom and spa cleaners.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Bathroom and spa cleaners. 2902.35 Section 2902.35... Items § 2902.35 Bathroom and spa cleaners. (a) Definition. Products that are designed to clean and/or prevent deposits on surfaces found in bathrooms and spas including, but not necessarily limited to,...

  1. Spa Goers' Repeated Visits for Health and Wellness and the Influential Factors: An Exploratory Study of the UK Spa Goers.

    PubMed

    Choi, Myungsuk; Poade, Donna; Sohn, Minsung; Choi, Mankyu

    2016-01-01

    Dissemination of spa services across the globe and its market saturation drive demand for differentiated communication methods. This study aims to explore the influential factors on spa goers' repeated visits and their practical applications in the health and wellness spa industry. The identified factors were used as the measurement variables to examine the relation with spa goers' repeated visits. The proposed concept was tested by a mixed method combining a self-administered questionnaire and semistructured interview with 54 survey participants and 6 interviewees. It was meaningful to use a sample of the UK spa goers from the southwest region since global spa trends stem from the EU spas, and the United Kingdom is one of the market leaders. The data were analyzed using frequency, percentage, multiple logistic regression analysis, and coding process. The survey findings demonstrated that the most significant influential factor on the repeated spa visits is a memorable experience of which showed 13.7 times higher probability than the reference up-to-date facility. The details of memorable experiences were discovered throughout the interviews that include the rediscovery of self, feeling of connectedness, recharge for positive emotions, self-reward through escapism, and experience of noncommercialized local products and attractions. Therefore, using experiential marketing methods can be effective spa service marketing. PMID:27455366

  2. Spa Goers' Repeated Visits for Health and Wellness and the Influential Factors: An Exploratory Study of the UK Spa Goers.

    PubMed

    Choi, Myungsuk; Poade, Donna; Sohn, Minsung; Choi, Mankyu

    2016-01-01

    Dissemination of spa services across the globe and its market saturation drive demand for differentiated communication methods. This study aims to explore the influential factors on spa goers' repeated visits and their practical applications in the health and wellness spa industry. The identified factors were used as the measurement variables to examine the relation with spa goers' repeated visits. The proposed concept was tested by a mixed method combining a self-administered questionnaire and semistructured interview with 54 survey participants and 6 interviewees. It was meaningful to use a sample of the UK spa goers from the southwest region since global spa trends stem from the EU spas, and the United Kingdom is one of the market leaders. The data were analyzed using frequency, percentage, multiple logistic regression analysis, and coding process. The survey findings demonstrated that the most significant influential factor on the repeated spa visits is a memorable experience of which showed 13.7 times higher probability than the reference up-to-date facility. The details of memorable experiences were discovered throughout the interviews that include the rediscovery of self, feeling of connectedness, recharge for positive emotions, self-reward through escapism, and experience of noncommercialized local products and attractions. Therefore, using experiential marketing methods can be effective spa service marketing.

  3. SPA Meteor Section Results: November -- December 1999

    NASA Astrophysics Data System (ADS)

    McBeath, A.

    2000-10-01

    Results and reports submitted to the SPA Meteor Section for November and December, 1999, are summarized, except for the Leonid details already discussed [1-3]. The Taurids received some useful coverage in early November, without anything unusual being detected. A brilliant fireball accurred around 22h10m UT on November 28 over southeast Ireland, after which about 271 g of L6 chondritic meteorites were recovered near Leighlinbridge, County Carlow, Ireland, the first recovered meteorite fall in the British Isles since 1991. Two other bright fireballs accurred on November 27-28 and 29-30 over Europe as well. December 13-14 saw the highest Geminid ZHRs, 100 +- 10, during a well-observed Geminid epoch. Radio data showed strong echo counts on both December 13 and 14, with the latter date (lambda approx 263 deg, eq. J2000.0) producing the highest counts generally, but with no specific clear maximum time. The Ursid peak was detected by radio only, giving a very weak showing in most data sets especially around lambda = 269-270 deg.

  4. CCD Photometry and Lightcurve Analysis of Main-Belt Asteroids 14 Irene 4874 Burke, 1985 Hopmann, 3017 Petrovic, and 3070 Aitken from Observatori Carmelita in Tiana

    NASA Astrophysics Data System (ADS)

    Aymami, Josep Maria

    2012-07-01

    Observations carried out from 2011 December to late 2012 March allowed us to determine the synodic periods of 14 Irene, 1985 Hopmann, 3017 Petrovic, 3040 Aitken, and 4874 Burke. For 14 Irene, a period of 15.038 ± 0.002 h was found with an amplitude A = 0.10 mag, well in accordance with other published estimates. 1985 Hopmann exhibited a rotational period of 17.476 ± 0.003 h, A = 0.44 mag. 3017 Petrovic exhibited a rotational period of 4.080 ± 0.001 h, A = 0.62 mag. 3070 Aitken showed a period of 6.390 ± 0.005 h, A = 0.59 mag. For 4874 Burke we found a short rotational period of 3.657 ± 0.001 h with A = 0.31 mag.

  5. [Global tendencies in the development of medical spa].

    PubMed

    Barashkova, G N; L'vova, N V; Persianova-Dubrova, A L; Krikorova, S A; Tupitsina, Iu Iu; Badalov, N G; Povazhnaia, E L

    2012-01-01

    The legal framework and the regulatory documents on which to base the development and activity of the medical spa in this country are practically absent which accounts for the necessity to analyse the global tendencies in this sphere. The authors review the literature data concerning the current trends in the development of health resort business and rehabilitative medicine and consider a new type of therapeutic and health-improving facilities known as "medical spa". The main aspects of the work of these centres with special reference to their therapeutic/preventive and organizational activities are discussed with due regard for the global experience and international practices in these fields.

  6. SPA: marketing and financial performance measures for the cow-calf enterprise.

    PubMed

    Gutierrez, P H

    1995-07-01

    The Standardized Performance Analysis (SPA) process can be an important business analysis activity. This article outlines the important considerations in terms of the process, necessary data, and interpretations and limitations of SPA measures of performance. A case ranch example is used to illustrate the SPA process.

  7. 75 FR 12167 - Virginia Graeme Baker Pool and Spa Safety Act; Public Accommodation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-15

    ... COMMISSION 16 CFR Part 1450 Virginia Graeme Baker Pool and Spa Safety Act; Public Accommodation AGENCY... ``public accommodation'' as used in the Virginia Graeme Baker Pool and Spa Safety Act. DATES: Written...; blittle@cpsc.gov . SUPPLEMENTARY INFORMATION: A. Background The Virginia Graeme Baker Pool and Spa...

  8. Scintillation proximity assay (SPA) technology to study biomolecular interactions.

    PubMed

    Cook, Neil; Harris, Alison; Hopkins, Alison; Hughes, Kelvin

    2002-05-01

    Scintillation proximity assay (SPA) is a versatile homogeneous technique for radioactive assays which eliminates the need for separation steps. In SPA, scintillant is incorporated into small fluomicrospheres. These microspheres or "beads" are constructed in such a way as to bind specific molecules. If a radioactive molecule is bound to the bead, it is brought into close enough proximity that it can stimulate the scintillant contained within to emit light. Otherwise, the unbound radioactivity is too distant, the energy released is dissipated before reaching the bead, and these disintegrations are not detected. In this unit, the application of SPA technology to measuring protein-protein interactions, Src Homology 2 (SH2) and 3 (SH3) domain binding to specific peptide sequences, and receptor-ligand interactions are described. Three other protocols discuss the application of SPA technology to cell-adhesion-molecule interactions, protein-DNA interactions, and radioimmunoassays. In addition, protocols are given for preparation of SK-N-MC cells and cell membranes. PMID:18429228

  9. A synoptic study of Sudden Phase Anomalies (SPA's) effecting VLF navigation and timing

    NASA Technical Reports Server (NTRS)

    Swanson, E. R.; Kugel, C. P.

    1973-01-01

    Sudden phase anomalies (SPA's) observed on VLF recordings are related to sudden ionospheric disturbances due to solar flares. Results are presented for SPA statistics on 500 events observed in New York during the ten year period 1961 to 1970. Signals were at 10.2kHz and 13.6kHz emitted from the OMEGA transmitters in Hawaii and Trinidad. A relationship between SPA frequency and sun spot number was observed. For sun spot number near 85, about one SPA per day will be observed somewhere in the world. SPA activity nearly vanishes during periods of low sun spot number. During years of high solar activity, phase perturbations observed near noon are dominated by SPA effects beyond the 95th percentile. The SPA's can be represented by a rapid phase run-off which is approximately linear in time, peaking in about 6 minutes, and followed by a linear recovery. Typical duration is 49 minutes.

  10. Characterization of the SpaCBA pilus fibers in the probiotic Lactobacillus rhamnosus GG.

    PubMed

    Reunanen, Justus; von Ossowski, Ingemar; Hendrickx, Antoni P A; Palva, Airi; de Vos, Willem M

    2012-04-01

    Lactobacillus rhamnosus GG is a human intestinal isolate that has been studied intensively because of its probiotic properties. We have previously shown that L. rhamnosus GG produces proteinaceous pili that earlier had been observed only in Gram-positive pathogens (M. Kankainen et al., Proc. Natl. Acad. Sci. U. S. A. 106:17193-17198, 2009). These pili were found to be encoded by the spaCBA gene cluster, and the pilus-associated SpaC pilin was shown to confer on the cells a mucus-binding ability. In addition to the spaCBA cluster, another putative pilus cluster, spaFED, was predicted from the L. rhamnosus GG genome sequence. Herein, we show that only SpaCBA pili are produced by L. rhamnosus, and we describe a detailed analysis of cell wall-associated and affinity-purified SpaCBA pili by Western blotting and immunogold electron microscopy. Our results indicate that SpaCBA pili are heterotrimeric protrusions with a SpaA subunit as the shaft-forming major pilin. Only a few SpaB subunits could be observed in pilus fibers. Instead, SpaB pilins were found at pilus bases, as assessed by immunogold double labeling of thin sections of cells, suggesting that SpaB is involved in the termination of pilus assembly. The SpaC adhesin was present along the whole pilus length at numbers nearly equaling those of SpaA. The relative amount and uniform distribution of SpaC within pili not only makes it possible to exert both long-distance and intimate contact with host tissue but also provides mucus-binding strength, which explains the prolonged intestinal residency times observed for L. rhamnosus GG compared to that of nonpiliated lactobacilli.

  11. DeFries-Fulker and Pearson-Aitken model-fitting analyses of reading performance data from selected and unselected twin pairs.

    PubMed

    Hawke, Jesse L; Stallings, Michael C; Wadsworth, Sally J; DeFries, John C

    2008-03-01

    Although a comparison of concordance rates for deviant scores in identical and fraternal twin pairs can provide prima facie evidence for a genetic etiology, information is not fully utilized when continuous measures are analyzed in a dichotomous manner. Thus, DeFries and Fulker (Behav Genet 15:467-473, 1985; Acta Genet Med Gemellol, 37:205-216, 1988) developed a regression-based methodology (DF analysis) to assess genetic etiology in both selected and unselected twin samples. While the DF analysis is a very versatile and relatively powerful statistical approach, it is not easily extended to the multivariate case. In contrast, structural equation models may be readily extended to analyze multivariate data sets (Neale and Cardon, Methodology for genetic studies of twins and families, 1992). However, such methodologies may yield biased estimates of additive genetic, shared environmental, and non-shared environmental influences when multivariate models are fitted to selected twin data. Therefore, the Pearson-Aitken (PA) selection formula (Aitken, Proc Edinburgh Math Soc B, 4:106-110, 1934) was used to analyze reading performance data from twins with reading difficulties (selected sample) and a population of normally-achieving twin pairs (control sample). As a comparison, DF models were also fitted to these same data sets. In general, resulting estimates of additive genetic, shared environmental, and non-shared environmental influences were similar when the DF and PA models were fitted to the data. However, the PA selection formula may be more readily generalized to the multivariate case.

  12. Light-Activated Phytochrome A and B Interact with Members of the SPA Family to Promote Photomorphogenesis in Arabidopsis by Reorganizing the COP1/SPA Complex

    PubMed Central

    Sheerin, David J.; Menon, Chiara; zur Oven-Krockhaus, Sven; Enderle, Beatrix; Zhu, Ling; Johnen, Philipp; Schleifenbaum, Frank; Stierhof, York-Dieter; Hiltbrunner, Andreas

    2015-01-01

    Phytochromes function as red/far-red photoreceptors in plants and are essential for light-regulated growth and development. Photomorphogenesis, the developmental program in light, is the default program in seed plants. In dark-grown seedlings, photomorphogenic growth is suppressed by the action of the CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1)/SUPPRESSOR OF phyA-105 (SPA) complex, which targets positive regulators of photomorphogenic growth for degradation by the proteasome. Phytochromes inhibit the COP1/SPA complex, leading to the accumulation of transcription factors promoting photomorphogenesis; yet, the mechanism by which they inactivate COP1/SPA is still unknown. Here, we show that light-activated phytochrome A (phyA) and phytochrome B (phyB) interact with SPA1 and other SPA proteins. Fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy analyses show that SPAs and phytochromes colocalize and interact in nuclear bodies. Furthermore, light-activated phyA and phyB disrupt the interaction between COP1 and SPAs, resulting in reorganization of the COP1/SPA complex in planta. The light-induced stabilization of HFR1, a photomorphogenic factor targeted for degradation by COP1/SPA, correlates temporally with the accumulation of phyA in the nucleus and localization of phyA to nuclear bodies. Overall, these data provide a molecular mechanism for the inactivation of the COP1/SPA complex by phyA- and phyB-mediated light perception. PMID:25627066

  13. 78 FR 33768 - Airworthiness Directives; Agusta S.p.A. (Type Certificate Currently Held by AgustaWestland S.p.A...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-05

    ... FR 11034, February 26, 1979); 3. Will not affect intrastate aviation in Alaska to the extent that it... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A. (Type Certificate Currently Held by AgustaWestland S.p.A.) Helicopters (Agusta) AGENCY: Federal...

  14. Light-activated phytochrome A and B interact with members of the SPA family to promote photomorphogenesis in Arabidopsis by reorganizing the COP1/SPA complex.

    PubMed

    Sheerin, David J; Menon, Chiara; zur Oven-Krockhaus, Sven; Enderle, Beatrix; Zhu, Ling; Johnen, Philipp; Schleifenbaum, Frank; Stierhof, York-Dieter; Huq, Enamul; Hiltbrunner, Andreas

    2015-01-01

    Phytochromes function as red/far-red photoreceptors in plants and are essential for light-regulated growth and development. Photomorphogenesis, the developmental program in light, is the default program in seed plants. In dark-grown seedlings, photomorphogenic growth is suppressed by the action of the CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1)/SUPPRESSOR OF phyA-105 (SPA) complex, which targets positive regulators of photomorphogenic growth for degradation by the proteasome. Phytochromes inhibit the COP1/SPA complex, leading to the accumulation of transcription factors promoting photomorphogenesis; yet, the mechanism by which they inactivate COP1/SPA is still unknown. Here, we show that light-activated phytochrome A (phyA) and phytochrome B (phyB) interact with SPA1 and other SPA proteins. Fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy analyses show that SPAs and phytochromes colocalize and interact in nuclear bodies. Furthermore, light-activated phyA and phyB disrupt the interaction between COP1 and SPAs, resulting in reorganization of the COP1/SPA complex in planta. The light-induced stabilization of HFR1, a photomorphogenic factor targeted for degradation by COP1/SPA, correlates temporally with the accumulation of phyA in the nucleus and localization of phyA to nuclear bodies. Overall, these data provide a molecular mechanism for the inactivation of the COP1/SPA complex by phyA- and phyB-mediated light perception.

  15. [An outbreak of Legionnaire's disease associated with a Japanese spa].

    PubMed

    Nakadate, T; Yamauchi, K; Inoue, H

    1999-08-01

    From June 24 to July 5, 1996, 3 patients were admitted to the same hospital with atypical pneumonia. One of the patients, a 52-year-old man, demonstrated progressive pulmonary infiltrates and severe hypoxemia, and finally required mechanical ventilation. All 3 patients had elevated antibody titers for Legionella pneumophila serogroup 1, and had visited the same spa prior to the onset of their symptoms. On September 25, 1996 the district health department inspected the spa, and isolated Legionella pneumophila serogroup 1 from the facility's hot water tanks and outlets. Although it has been reported that many spas in Japan are contaminated with Legionellaceae, the outbreak we encountered suggests that Japanese spas, like whirlpool spas in Europe and North America, can be a source of Legionnaire's disease.

  16. Generalized Scalar Multiplication Secure against SPA, DPA, and RPA

    NASA Astrophysics Data System (ADS)

    Miyaji, Atsuko

    In the execution on a smart card, elliptic curve cryptosystems have to be secure against side channel attacks such as the simple power analysis (SPA), the differential power analysis (DPA), and the refined power analysis (RPA), and so on. MMM-algorithm proposed by Mamiya, Miyaji, and Morimoto is a scalar multiplication algorithm secure against SPA, DPA, and RPA, which can decrease the computational complexity by increasing the size of a pre-computed table. However, it provides only 4 different cases of pre-computed tables. From the practical point of view, a wider range of time-memory tradeoffs is usually desired. This paper generalizes MMM-algorithm to improve the flexibility of tables as well as the computational complexity. Our improved algorithm is secure, efficient and flexible for the storage size.

  17. Lactobacillus rhamnosus GG SpaC pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates.

    PubMed

    Nishiyama, Keita; Ueno, Shintaro; Sugiyama, Makoto; Yamamoto, Yuji; Mukai, Takao

    2016-06-01

    Lactobacillus rhamnosus GG (LGG) is a well-established probiotic strain. The beneficial properties of this strain are partially dependent on its prolonged residence in the gastrointestinal tract, and are likely influenced by its adhesion to the intestinal mucosa. The pilin SpaC subunit, located within the Spa pili structure, is the most well studied LGG adhesion factor. However, the binding epitopes of SpaC remain largely unknown. The aim of this study was to evaluate the binding properties of SpaC to the carbohydrate moieties of intestinal glycoconjugates using a recombinant SpaC protein. In a competitive enzyme-linked immunosorbent assay, SpaC binding was markedly reduced by addition of purified mucin and the mucin oligosaccharide fraction. Histochemical staining revealed that the binding of SpaC was drastically reduced by periodic acid treatment. Moreover, in the surface plasmon resonance-based Biacore assay, SpaC bound strongly to the carbohydrate moieties containing β-galactoside at the non-reducing terminus of glycolipids. We here provide the first demonstration that SpaC binds to the oligosaccharide chains of mucins, and that the carbohydrate moieties containing β-galactoside at the non-reducing termini of glycoconjugates play a crucial role in this binding. Our results demonstrate the importance of carbohydrates of SpaC for mucus interactions.

  18. SPA-1 controls the invasion and metastasis of human prostate cancer.

    PubMed

    Shimizu, Yosuke; Hamazaki, Yoko; Hattori, Masakazu; Doi, Keiko; Terada, Naoki; Kobayashi, Takashi; Toda, Yoshinobu; Yamasaki, Toshinari; Inoue, Takahiro; Kajita, Yoichiro; Maeno, Atsushi; Kamba, Tomomi; Mikami, Yoshiki; Kamoto, Toshiyuki; Yamada, Tomomi; Kanno, Toru; Yoshikawa, Kiyotsugu; Ogawa, Osamu; Minato, Nagahiro; Nakamura, Eijiro

    2011-04-01

    Recent studies suggest that SIPA1 encoding a Rap GTPase-activating protein SPA-1 is a candidate metastasis efficiency-modifying gene in human breast cancer. In this study, we investigated the expression and function of SPA-1 in human prostate cancer (CaP). Immunohistochemical studies of tumor specimens from CaP patients revealed a positive correlation of SPA-1 expression with disease progression and metastasis. The correlation was recapitulated in human CaP cell lines; LNCaP that rarely showed metastasis in SCID mice expressed an undetectable level of SPA-1, whereas highly metastatic PC3 showed abundant SPA-1 expression. Moreover, SIPA1 transduction in LNCaP caused prominent abdominal lymph node metastasis without affecting primary tumor size, whereas shRNA-mediated SIPA1 knockdown or expression of a dominant-active Rap1 mutant (Rap1V12) in PC3 suppressed metastasis. LNCaP transduced with SPA-1 (LNCaP/SPA-1) showed attenuated adhesion to the precoated extracellular matrices (ECM) including collagens and fibronectin, due to defective ECM-medicated Rap1 activation. In addition, LNCaP/SPA-1 showed a diminished level of nuclear Brd4, which is known to bind SPA-1, resulting in reduced expression of a series of ECM-related genes. These results suggest that SPA-1 plays an important role in controlling metastasis efficiency of human CaP by regulating the expression of and interaction with ECM in the primary sites.

  19. Heat pump assisted geothermal heating system for Felix Spa, Romania

    SciTech Connect

    Rosca, Marcel; Maghiar, Teodor

    1996-01-24

    The paper presents a pre-feasibility type study of a proposed heat pump assisted geothermal heating system for an average hotel in Felix Spa, Romania. After a brief presentation of the geothermal reservoir, the paper gives the methodology and the results of the technical and economical calculations. The technical and economical viability of the proposed system is discussed in detail in the final part of the paper.

  20. Using color histograms and SPA-LDA to classify bacteria.

    PubMed

    de Almeida, Valber Elias; da Costa, Gean Bezerra; de Sousa Fernandes, David Douglas; Gonçalves Dias Diniz, Paulo Henrique; Brandão, Deysiane; de Medeiros, Ana Claudia Dantas; Véras, Germano

    2014-09-01

    In this work, a new approach is proposed to verify the differentiating characteristics of five bacteria (Escherichia coli, Enterococcus faecalis, Streptococcus salivarius, Streptococcus oralis, and Staphylococcus aureus) by using digital images obtained with a simple webcam and variable selection by the Successive Projections Algorithm associated with Linear Discriminant Analysis (SPA-LDA). In this sense, color histograms in the red-green-blue (RGB), hue-saturation-value (HSV), and grayscale channels and their combinations were used as input data, and statistically evaluated by using different multivariate classifiers (Soft Independent Modeling by Class Analogy (SIMCA), Principal Component Analysis-Linear Discriminant Analysis (PCA-LDA), Partial Least Squares Discriminant Analysis (PLS-DA) and Successive Projections Algorithm-Linear Discriminant Analysis (SPA-LDA)). The bacteria strains were cultivated in a nutritive blood agar base layer for 24 h by following the Brazilian Pharmacopoeia, maintaining the status of cell growth and the nature of nutrient solutions under the same conditions. The best result in classification was obtained by using RGB and SPA-LDA, which reached 94 and 100 % of classification accuracy in the training and test sets, respectively. This result is extremely positive from the viewpoint of routine clinical analyses, because it avoids bacterial identification based on phenotypic identification of the causative organism using Gram staining, culture, and biochemical proofs. Therefore, the proposed method presents inherent advantages, promoting a simpler, faster, and low-cost alternative for bacterial identification.

  1. Supersymmetry Parameter Analysis: SPA Convention andProject

    SciTech Connect

    Aguilar-Saavedra, J.A.; Ali, A.; Allanach, B.C.; Arnowitt, R.; Baer, H.A.; Bagger, J.A.; Balazs, C.; Barger, V.; Barnett, M.; Bartl, A.; Battaglia, M.; Bechtle, P.; Belanger, G.; Belyaev, A.; Berger, E.L.; Blair, G.; Boos, E.; Carena, M.; Choi, S.Y.; Deppisch, F.; De Roeck, A.; /Lisbon, IST /DESY /Cambridge U., DAMTP /Texas A-M /Florida State U. /Johns Hopkins U. /Argonne /Wisconsin U., Madison /LBL, Berkeley /Vienna U. /SLAC /Annecy, LAPTH /Michigan State U. /Royal Holloway, U. of London /SINP, Moscow /Fermilab /Chonbuk Natl. U. /CERN /Freiburg U. /Chile U., Catolica /Orsay, LAL

    2005-12-02

    High-precision analyses of supersymmetry parameters aim at reconstructing the fundamental supersymmetric theory and its breaking mechanism. A well defined theoretical framework is needed when higher-order corrections are included. We propose such a scheme, Supersymmetry Parameter Analysis SPA, based on a consistent set of conventions and input parameters. A repository for computer programs is provided which connect parameters in different schemes and relate the Lagrangian parameters to physical observables at LHC and high energy e{sup +}e{sup -} linear collider experiments, i.e., masses, mixings, decay widths and production cross sections for supersymmetric particles. In addition, programs for calculating high-precision low energy observables, the density of cold dark matter (CDM) in the universe as well as the cross sections for CDM search experiments are included. The SPA scheme still requires extended efforts on both the theoretical and experimental side before data can be evaluated in the future at the level of the desired precision. We take here an initial step of testing the SPA scheme by applying the techniques involved to a specific supersymmetry reference point.

  2. The "Support to Precursor Space Situational Awareness Services" (SPA) Project

    NASA Astrophysics Data System (ADS)

    Valero, J. L.; Albani, S.; Gallardo, B.; Matute, J.; O'Dwyer, A.

    2012-01-01

    Space activities are increasingly important in areas such as environment, science, navigation and security. Space Situational Awareness (SSA) refers to the knowledge of location and function of space objects and the space environment. The development of an SSA capability will allow the European Union (EU) and its Member States to better use space, strengthening their security and economy. The "Support to Precursor space situational Awareness services" (SPA) project is an FP7 Support Action managed by the European Union Satellite Centre (EUSC) under the full control of EU Member States and implemented with the collaboration of SSA Key Stakeholders. SPA is studying possible SSA Governance and Data Policy models in the EUSC secure environment by experimenting with a number of SSA preliminary services relevant to civilian security and to the Common Foreign and Security Policy (CFSP) such as Satellite Over-flight, Satellite Conjunction Warning and Space Re-entry Prediction. The final output of the SPA project will be a report providing recommendations for further development of SSA in Europe, particularly on the technical aspects of its Governance and Data Policy.

  3. Using color histograms and SPA-LDA to classify bacteria.

    PubMed

    de Almeida, Valber Elias; da Costa, Gean Bezerra; de Sousa Fernandes, David Douglas; Gonçalves Dias Diniz, Paulo Henrique; Brandão, Deysiane; de Medeiros, Ana Claudia Dantas; Véras, Germano

    2014-09-01

    In this work, a new approach is proposed to verify the differentiating characteristics of five bacteria (Escherichia coli, Enterococcus faecalis, Streptococcus salivarius, Streptococcus oralis, and Staphylococcus aureus) by using digital images obtained with a simple webcam and variable selection by the Successive Projections Algorithm associated with Linear Discriminant Analysis (SPA-LDA). In this sense, color histograms in the red-green-blue (RGB), hue-saturation-value (HSV), and grayscale channels and their combinations were used as input data, and statistically evaluated by using different multivariate classifiers (Soft Independent Modeling by Class Analogy (SIMCA), Principal Component Analysis-Linear Discriminant Analysis (PCA-LDA), Partial Least Squares Discriminant Analysis (PLS-DA) and Successive Projections Algorithm-Linear Discriminant Analysis (SPA-LDA)). The bacteria strains were cultivated in a nutritive blood agar base layer for 24 h by following the Brazilian Pharmacopoeia, maintaining the status of cell growth and the nature of nutrient solutions under the same conditions. The best result in classification was obtained by using RGB and SPA-LDA, which reached 94 and 100 % of classification accuracy in the training and test sets, respectively. This result is extremely positive from the viewpoint of routine clinical analyses, because it avoids bacterial identification based on phenotypic identification of the causative organism using Gram staining, culture, and biochemical proofs. Therefore, the proposed method presents inherent advantages, promoting a simpler, faster, and low-cost alternative for bacterial identification. PMID:25023972

  4. Lunar and Planetary Science XXXV: Lunar Crust as Sampled by Basins and Craters

    NASA Technical Reports Server (NTRS)

    2004-01-01

    The session "Lunar Crust as Samples by Basins and Craters" included:Radar Properties of Lunar Basin Deposits; Numerical Modeling of the South Pole-Aitkin Impact; Lunar South Pole-Aitken Impact Basin: Topography and Mineralogy; Comparison of the Geologic Setting of the South Pole-Aitken Basin Interior with Apollo 16: Implications for Regolith Components; Identifying Impact Events Within the Lunar Cataclysm from 40Ar-39Ar Ages of Apollo 16 Impact Melt Rocks; Apollo 16 Mafic Glass: Geochemistry, Provenance, and Implications; Lunar Meteorite PCA 02 007: A Feldspathic Regolith Breccia with Mixed Mare/Highland Components; Compositional Constraints on the Launch Pairing of LAP 02205 and PCA 02007 with Other Lunar Meteorites; An In-Situ Study of REE Abundances in Three Anorthositic Impact Melt Lunar Highland Meteorites; A Crustal Rock Clast in Magnesian Anorthositic Breccia, Dhofar 489 and Its Excavation from a Large Basin; The Origin and Impact History of Lunar Meteorite Yamato 86032; Evolved Lithologies and Their Inferred Sources in the Northwestern Procellarum Region of the Moon; and Revisiting the Interpretation of Thorium Abundances at Hansteen Alpha.

  5. Human Pulmonary Surfactant Protein SP-A1 Provides Maximal Efficiency of Lung Interfacial Films.

    PubMed

    Lopez-Rodriguez, Elena; Pascual, Alicia; Arroyo, Raquel; Floros, Joanna; Perez-Gil, Jesus

    2016-08-01

    Pulmonary surfactant is a lipoprotein complex that reduces surface tension to prevent alveolar collapse and contributes to the protection of the respiratory surface from the entry of pathogens. Surfactant protein A (SP-A) is a hydrophilic glycoprotein of the collectin family, and its main function is related to host defense. However, previous studies have shown that SP-A also aids in the formation and biophysical properties of pulmonary surfactant films at the air-water interface. Humans, unlike rodents, have two genes, SFTPA1 and SFTPA2. The encoded proteins, SP-A1 and SP-A2, differ quantitatively or qualitatively in function. It has been shown that both gene products are necessary for tubular myelin formation, an extracellular structural form of lung surfactant. The goal of this study was to investigate potential differences in the biophysical properties of surfactants containing human SP-A1, SP-A2, or both. For this purpose, we have studied for the first time, to our knowledge, the biophysical properties of pulmonary surfactant from individual humanized transgenic mice expressing human SP-A1, SP-A2, or both SP-A1 and SP-A2, in the captive bubble surfactometer. We observed that pulmonary surfactant containing SP-A1 reaches lower surface tension after postexpansion interfacial adsorption than surfactants containing no SP-A or only SP-A2. Under interfacial compression-expansion cycling conditions, surfactant films containing SP-A1 also performed better, particularly with respect to the reorganization of the films that takes place during compression. On the other hand, addition of recombinant SP-A1 to a surfactant preparation reconstituted from the hydrophobic fraction of a porcine surfactant made it more resistant to inhibition by serum than the addition of equivalent amounts of SP-A2. We conclude that the presence of SP-A1 allows pulmonary surfactant to adopt a particularly favorable structure with optimal biophysical properties.

  6. Human Pulmonary Surfactant Protein SP-A1 Provides Maximal Efficiency of Lung Interfacial Films.

    PubMed

    Lopez-Rodriguez, Elena; Pascual, Alicia; Arroyo, Raquel; Floros, Joanna; Perez-Gil, Jesus

    2016-08-01

    Pulmonary surfactant is a lipoprotein complex that reduces surface tension to prevent alveolar collapse and contributes to the protection of the respiratory surface from the entry of pathogens. Surfactant protein A (SP-A) is a hydrophilic glycoprotein of the collectin family, and its main function is related to host defense. However, previous studies have shown that SP-A also aids in the formation and biophysical properties of pulmonary surfactant films at the air-water interface. Humans, unlike rodents, have two genes, SFTPA1 and SFTPA2. The encoded proteins, SP-A1 and SP-A2, differ quantitatively or qualitatively in function. It has been shown that both gene products are necessary for tubular myelin formation, an extracellular structural form of lung surfactant. The goal of this study was to investigate potential differences in the biophysical properties of surfactants containing human SP-A1, SP-A2, or both. For this purpose, we have studied for the first time, to our knowledge, the biophysical properties of pulmonary surfactant from individual humanized transgenic mice expressing human SP-A1, SP-A2, or both SP-A1 and SP-A2, in the captive bubble surfactometer. We observed that pulmonary surfactant containing SP-A1 reaches lower surface tension after postexpansion interfacial adsorption than surfactants containing no SP-A or only SP-A2. Under interfacial compression-expansion cycling conditions, surfactant films containing SP-A1 also performed better, particularly with respect to the reorganization of the films that takes place during compression. On the other hand, addition of recombinant SP-A1 to a surfactant preparation reconstituted from the hydrophobic fraction of a porcine surfactant made it more resistant to inhibition by serum than the addition of equivalent amounts of SP-A2. We conclude that the presence of SP-A1 allows pulmonary surfactant to adopt a particularly favorable structure with optimal biophysical properties. PMID:27508436

  7. Assessing the quality of study reports on spa therapy based on randomized controlled trials by the spa therapy checklist (SPAC).

    PubMed

    Kamioka, Hiroharu; Tsutani, Kiichiro; Maeda, Masaharu; Hayasaka, Shinya; Okuizum, Hiroyasu; Goto, Yasuaki; Okada, Shinpei; Kitayuguchi, Jun; Abe, Takafumi

    2014-11-01

    The purpose of this study was to assess the quality of study reports on spa therapy based on randomized controlled trials by the spa therapy and balneotherapy checklist (SPAC), and to show the relationship between SPAC score and the characteristics of publication. We searched the following databases from 1990 up to September 30, 2013: MEDLINE via PubMed, CINAHL, Web of Science, Ichushi Web, Global Health Library, the Western Pacific Region Index Medicus, PsycINFO, and the Cochrane Database of Systematic Reviews. We used the SPAC to assess the quality of reports on spa therapy and balneotherapy trials (SPAC) that was developed using the Delphi consensus method. Fifty-one studies met all inclusion criteria. Forty studies (78%) were about "Diseases of the musculoskeletal system and connective)". The total SPAC score (full-mark; 19 pts) was 10.8 ± 2.3 pts (mean ± SD). The items for which a description was lacking (very poor; <50%) in many studies were as follows: "locations of spa facility where the data were collected"; "pH"; "scale of bathtub"; "presence of other facility and exposure than bathing (sauna, steam bath, etc.)"; "qualification and experience of care provider"; "Instructions about daily life" and "adherence". We clarified that there was no relationship between the publish period, languages, and the impact factor (IF) for the SPAC score. In order to prevent flawed description, SPAC could provide indispensable information for researchers who are going to design a research protocol according to each disease.

  8. Functional interconnection of MYC2 and SPA1 in the photomorphogenic seedling development of Arabidopsis.

    PubMed

    Gangappa, Sreeramaiah N; Prasad, V Babu Rajendra; Chattopadhyay, Sudip

    2010-11-01

    MYC2 is a basic helix-loop-helix transcription factor that cross talks with light, abscisic acid (ABA), and jasmonic acid (JA) signaling pathways. Here, we have shown that Arabidopsis (Arabidopsis thaliana) MYC2 directly binds to the G-box present in the SUPPRESSOR OF PHYTOCHROME A1 (SPA1) promoter and that it controls the expression of SPA1 in a COP1-dependent manner. Analyses of atmyc2 spa1 double mutants suggest that whereas MYC2 and SPA1 act redundantly to suppress photomorphogenic growth in the dark, they function synergistically for the suppression of photomorphogenic growth in the light. Our studies have also revealed that MYC2-mediated ABA and JA responses are further modulated by SPA1. Taken together, this study demonstrates the molecular and physiological interrelations of MYC2 and SPA1 in light, ABA, and JA signaling pathways.

  9. Improved protocol for rapid identification of certain spa types using high resolution melting curve analysis.

    PubMed

    Mayerhofer, Benjamin; Stöger, Anna; Pietzka, Ariane T; Fernandez, Haizpea Lasa; Prewein, Bernhard; Sorschag, Sieglinde; Kunert, Renate; Allerberger, Franz; Ruppitsch, Werner

    2015-01-01

    Methicillin-resistant Staphylococcus aureus is one of the most significant pathogens associated with health care. For efficient surveillance, control and outbreak investigation, S. aureus typing is essential. A high resolution melting curve analysis was developed and evaluated for rapid identification of the most frequent spa types found in an Austrian hospital consortium covering 2,435 beds. Among 557 methicillin-resistant Staphylococcus aureus isolates 38 different spa types were identified by sequence analysis of the hypervariable region X of the protein A gene (spa). Identification of spa types through their characteristic high resolution melting curve profiles was considerably improved by double spiking with genomic DNA from spa type t030 and spa type t003 and allowed unambiguous and fast identification of the ten most frequent spa types t001 (58%), t003 (12%), t190 (9%), t041 (5%), t022 (2%), t032 (2%), t008 (2%), t002 (1%), t5712 (1%) and t2203 (1%), representing 93% of all isolates within this hospital consortium. The performance of the assay was evaluated by testing samples with unknown spa types from the daily routine and by testing three different high resolution melting curve analysis real-time PCR instruments. The ten most frequent spa types were identified from all samples and on all instruments with 100% specificity and 100% sensitivity. Compared to classical spa typing by sequence analysis, this gene scanning assay is faster, cheaper and can be performed in a single closed tube assay format. Therefore it is an optimal screening tool to detect the most frequent endemic spa types and to exclude non-endemic spa types within a hospital. PMID:25768007

  10. Improved protocol for rapid identification of certain spa types using high resolution melting curve analysis.

    PubMed

    Mayerhofer, Benjamin; Stöger, Anna; Pietzka, Ariane T; Fernandez, Haizpea Lasa; Prewein, Bernhard; Sorschag, Sieglinde; Kunert, Renate; Allerberger, Franz; Ruppitsch, Werner

    2015-01-01

    Methicillin-resistant Staphylococcus aureus is one of the most significant pathogens associated with health care. For efficient surveillance, control and outbreak investigation, S. aureus typing is essential. A high resolution melting curve analysis was developed and evaluated for rapid identification of the most frequent spa types found in an Austrian hospital consortium covering 2,435 beds. Among 557 methicillin-resistant Staphylococcus aureus isolates 38 different spa types were identified by sequence analysis of the hypervariable region X of the protein A gene (spa). Identification of spa types through their characteristic high resolution melting curve profiles was considerably improved by double spiking with genomic DNA from spa type t030 and spa type t003 and allowed unambiguous and fast identification of the ten most frequent spa types t001 (58%), t003 (12%), t190 (9%), t041 (5%), t022 (2%), t032 (2%), t008 (2%), t002 (1%), t5712 (1%) and t2203 (1%), representing 93% of all isolates within this hospital consortium. The performance of the assay was evaluated by testing samples with unknown spa types from the daily routine and by testing three different high resolution melting curve analysis real-time PCR instruments. The ten most frequent spa types were identified from all samples and on all instruments with 100% specificity and 100% sensitivity. Compared to classical spa typing by sequence analysis, this gene scanning assay is faster, cheaper and can be performed in a single closed tube assay format. Therefore it is an optimal screening tool to detect the most frequent endemic spa types and to exclude non-endemic spa types within a hospital.

  11. Surfactant protein (SP)-A and SP-D as antimicrobial and immunotherapeutic agents.

    PubMed

    Awasthi, Shanjana

    2010-06-01

    Surfactant protein (SP)-A and SP-D belong to the "Soluble C-type Lectin" family of proteins and are collectively known as "Collectins". Based on their ability to recognize pathogens and to regulate the host defense, SP-A and SP-D have been recently categorized as "Secretory Pathogen Recognition Receptors". SP-A and SP-D were first identified in the lung; the expression of SP-A and SP-D has also been observed at other mucosal surfaces, such as lacrimal glands, gastrointestinal mucosa, genitourinary epithelium and periodontal surfaces. Since the role of these proteins is not fully elucidated at other mucosal surfaces, the focus of this article is on lung-SP-A and SP-D. It has become clear from research studies performed over a number of years that SP-A and SP-D are critical for the maintenance of lung homeostasis and the regulation of host defense and inflammation. However, none of the surfactant preparations available for clinical use have SP-A or SP-D. A review is presented here on SP-A- and SP-D-deficiencies in lung diseases, the importance of the administration of SP-A and SP-D, and recent patents and research directions that may lead to the design of novel SP-A- or SP-D-based therapeutics and surfactants.

  12. Transcriptional profiling of XdrA, a new regulator of spa transcription in Staphylococcus aureus.

    PubMed

    McCallum, N; Hinds, J; Ender, M; Berger-Bächi, B; Stutzmann Meier, P

    2010-10-01

    Transcription of spa, encoding the virulence factor protein A in Staphylococcus aureus, is tightly controlled by a complex regulatory network, ensuring its temporal expression over growth and at appropriate stages of the infection process. Transcriptomic profiling of XdrA, a DNA-binding protein that is conserved in all S. aureus genomes and shares similarity with the XRE family of helix-turn-helix, antitoxin-like proteins, revealed it to be a previously unidentified activator of spa transcription. To assess how XdrA fits into the complex web of spa regulation, a series of regulatory mutants were constructed; consisting of single, double, triple, and quadruple mutants lacking XdrA and/or the three key regulators previously shown to influence spa transcription directly (SarS, SarA, and RNAIII). A series of lacZ reporter gene fusions containing nested deletions of the spa promoter identified regions influenced by XdrA and the other three regulators. XdrA had almost as strong an activating effect on spa as SarS and acted on the same spa operator regions as SarS, or closely overlapping regions. All data from microarrays, Northern and Western blot analyses, and reporter gene fusion experiments indicated that XdrA is a major activator of spa expression that appears to act directly on the spa promoter and not through previously characterized regulators.

  13. 'MRI-tis' in the early diagnosis of axial SpA: issues and limitations.

    PubMed

    De Rycke, Leen; Maas, Mario; Tak, Paul P; Baeten, Dominique

    2010-11-01

    Sacroiliitis on conventional radiography, a key diagnostic feature of axial spondyloarthritis (SpA), often appears only late in the disease course. With the introduction of potent biologic agents that may also be effective in early disease, diagnostic techniques that can identify SpA early in the disease course would be highly beneficial to patients. MRI has been proposed as a novel diagnostic tool for early axial SpA based on the visualization of active inflammatory lesions in established axial SpA, as well as in pre-radiographic axial SpA. Accordingly, MRI is already widely used in clinical practice and has been included in new classification criteria. However, the specificity and predictive value of MRI lesions for the development of axial SpA remain to be fully defined and validated. Most data come from cross-sectional analyses and have not been validated in prospective studies, and the few available prospective studies were performed in highly selected patient populations and have assessed the value of MRI for the prediction of sacroiliitis rather than axial SpA. Also, some studies have indicated considerable diversity in the pattern and extent of MRI lesions, and suggest that many lesions are not specific for SpA. Prospective, longitudinal studies are needed to validate the utility of this new imaging modality for the diagnosis of axial SpA.

  14. Phenotypical Analysis of the Lactobacillus rhamnosus GG Fimbrial spaFED Operon: Surface Expression and Functional Characterization of Recombinant SpaFED Pili in Lactococcus lactis

    PubMed Central

    Kant, Ravi; Palva, Airi; von Ossowski, Ingemar

    2014-01-01

    A noticeable genomic feature of many piliated Gram-positive bacterial species is the presence of more than one pilus-encoding operon. Paradigmatically, the gut-adapted Lactobacillus rhamnosus GG strain contains two different fimbrial operons in its genome. However, whereas one of these operons (called spaCBA) is encoding for the functionally mucus-/collagen-binding SpaCBA pilus, for the other operon (called spaFED) any native expression of the SpaFED-called pili is still the subject of some uncertainty. Irrespective of such considerations, we decided it would be of relevance or interest to decipher the gross structure of this pilus type, and as well assess its functional capabilities for cellular adhesion and immunostimulation. For this, and by following the approach we had used previously to explicate the immuno-properties of SpaCBA pili, we constructed nisin-inducible expression clones producing either wild-type or SpaF pilin-deleted surface-assembled L. rhamnosus GG SpaFED pili on Lactococcus lactis cells. Using these piliated lactococcal constructs, we found that the pilin-polymerized architecture of a recombinant-produced SpaFED pilus coincides with sequence-based functional predictions of the related pilins, and in fact is prototypical of those other sortase-dependent pilus-like structures thus far characterized for piliated Gram-positive bacteria. Moreover, we confirmed that among the different pilin subunits encompassing spaFED operon-encoded pili, the SpaF pilin is a main adhesion determinant, and when present in the assembled structure can mediate pilus binding to mucus, certain extracellular matrix proteins, and different gut epithelial cell lines. However, somewhat unexpectedly, when recombinant SpaFED pili are surface-attached, we found that they could not potentiate the existing lactococcal cell-induced immune responses so elicited from intestinal- and immune-related cells, but rather instead, they could dampen them. Accordingly, we have now provided

  15. Phenotypical analysis of the Lactobacillus rhamnosus GG fimbrial spaFED operon: surface expression and functional characterization of recombinant SpaFED pili in Lactococcus lactis.

    PubMed

    Rintahaka, Johanna; Yu, Xia; Kant, Ravi; Palva, Airi; von Ossowski, Ingemar

    2014-01-01

    A noticeable genomic feature of many piliated Gram-positive bacterial species is the presence of more than one pilus-encoding operon. Paradigmatically, the gut-adapted Lactobacillus rhamnosus GG strain contains two different fimbrial operons in its genome. However, whereas one of these operons (called spaCBA) is encoding for the functionally mucus-/collagen-binding SpaCBA pilus, for the other operon (called spaFED) any native expression of the SpaFED-called pili is still the subject of some uncertainty. Irrespective of such considerations, we decided it would be of relevance or interest to decipher the gross structure of this pilus type, and as well assess its functional capabilities for cellular adhesion and immunostimulation. For this, and by following the approach we had used previously to explicate the immuno-properties of SpaCBA pili, we constructed nisin-inducible expression clones producing either wild-type or SpaF pilin-deleted surface-assembled L. rhamnosus GG SpaFED pili on Lactococcus lactis cells. Using these piliated lactococcal constructs, we found that the pilin-polymerized architecture of a recombinant-produced SpaFED pilus coincides with sequence-based functional predictions of the related pilins, and in fact is prototypical of those other sortase-dependent pilus-like structures thus far characterized for piliated Gram-positive bacteria. Moreover, we confirmed that among the different pilin subunits encompassing spaFED operon-encoded pili, the SpaF pilin is a main adhesion determinant, and when present in the assembled structure can mediate pilus binding to mucus, certain extracellular matrix proteins, and different gut epithelial cell lines. However, somewhat unexpectedly, when recombinant SpaFED pili are surface-attached, we found that they could not potentiate the existing lactococcal cell-induced immune responses so elicited from intestinal- and immune-related cells, but rather instead, they could dampen them. Accordingly, we have now provided

  16. A online credit evaluation method based on AHP and SPA

    NASA Astrophysics Data System (ADS)

    Xu, Yingtao; Zhang, Ying

    2009-07-01

    Online credit evaluation is the foundation for the establishment of trust and for the management of risk between buyers and sellers in e-commerce. In this paper, a new credit evaluation method based on the analytic hierarchy process (AHP) and the set pair analysis (SPA) is presented to determine the credibility of the electronic commerce participants. It solves some of the drawbacks found in classical credit evaluation methods and broadens the scope of current approaches. Both qualitative and quantitative indicators are considered in the proposed method, then a overall credit score is achieved from the optimal perspective. In the end, a case analysis of China Garment Network is provided for illustrative purposes.

  17. Environmental factors and the development of Bath Spa, England

    NASA Astrophysics Data System (ADS)

    Kellaway, G. A.

    1994-10-01

    Thermal water springs at Bristol and Bath in west England have come under close scrutiny since the closure of Bath Spa in 1978. In order to protect the hot springs from dewatering and loss of pressure due to largescale quarrying and deep drilling, it is necessary to identify the sources and routes whereby the thermal water travels to its resurgences in the Avon valley. Control over deep water movements is exercised by the structure and size of the aquifers and aquicludes, modified by zones of Quaternary—Recent fracturing along which water movements have not been restricted or blocked by mineralization.

  18. 77 FR 3382 - Airworthiness Directives; Agusta S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-24

    ...-054-AD; Amendment 39-16910; AD 2011-27-08] RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A... rule; request for comments. SUMMARY: We are adopting a new airworthiness directive (AD) for all Agusta S.p.A. (Agusta) model A109S and AW109SP helicopters. This AD is prompted by a fatigue crack found...

  19. 77 FR 55684 - Airworthiness Directives; Agusta S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-11

    ...-052-AD; Amendment 39-17178; AD 2012-18-02] RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A... rule; request for comments. SUMMARY: We are adopting a new airworthiness directive (AD) for Agusta S.p.A. (Agusta) Model AB412 and AB412 EP helicopters with certain hoist hook assemblies (hook)...

  20. 77 FR 30236 - Airworthiness Directives; Agusta S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-22

    ... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A...). SUMMARY: We propose to adopt a new airworthiness directive (AD) for certain Agusta S.p.A. (Agusta)...

  1. 78 FR 41005 - Airworthiness Directives; Vulcanair S.p.A. Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-09

    ... (73 FR 72314; November 28, 2008). (c) Applicability This AD applies to Vulcanair S.p.A. Models P 68, P... Directives; Vulcanair S.p.A. Airplanes AGENCY: Federal Aviation Administration (FAA), Department of Transportation (DOT). ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: We propose to adopt a...

  2. 77 FR 36137 - Airworthiness Directives; AGUSTA S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-18

    ...-017-AD; Amendment 39-17076; AD 2012-11-12] RIN 2120-AA64 Airworthiness Directives; AGUSTA S.p.A... rule; request for comments. SUMMARY: We are adopting a new airworthiness directive (AD) for Agusta S.p.A. (Agusta) Model AW139 helicopters to determine if the hardware that attaches the upper end...

  3. 77 FR 20518 - Airworthiness Directives; Agusta S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-05

    ... 2011-SW-013-AD; Amendment 39-17007; AD 2012-07-01] RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A... rule; request for comments. SUMMARY: We are adopting a new airworthiness directive (AD) for Agusta S.p.A. (Agusta) Model AB412 helicopters with certain tail rotor blades (blades) installed. This...

  4. 77 FR 64699 - Airworthiness Directives; Agusta S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-23

    ...-016-AD; Amendment 39-17223; AD 2012-21-07] RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A. Helicopters AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: We are adopting a new airworthiness directive (AD) for Agusta S.p.A. (Agusta) Model A109S helicopters to...

  5. Hydrochemical Investigation of the Balikli Spa (Kangal Fish Spring), Sivas, Turkey

    NASA Astrophysics Data System (ADS)

    Kaçaroǧlu, Fikret

    2010-05-01

    Balıklı Spa (Kangal Fish Spring) is situated to the northeast of Kangal district centre, Sivas, Turkey. Balıklı Spa is of a particular place amongst the other thermal baths found in Turkey and is used in the treatment of psoriasis. The temperature and discharge of the Balıklı Spa water range between 33.6 and 35.4 oC and 145-220 L/s, respectively. The discharge of the Çermik Stream measured downstream of the Balıklı Spa is in the range of 150-660 L/s, and is mostly supplied by Balıklı Spa. Total dissolved solids (TDS) of the Balıklı Spa and Çermik Stream waters range between 348 and 395 mg/L and 278 and 377 mg/L, respectively. Dominant ions of these waters are Ca, Mg and HCO3, and they are classified as calcium bicarbonate type waters. In terms of balneological classification Balıklı Spa is "acrotothermal water". Secondary and minor element (B, Cr, Mn, Fe, Cu, Zn, As, Se, Sr, Sb, Ba, Hg, Pb) concentrations in the investigated waters are below 1 mg/L. The stable isotope (oxygen-18 and deuterium) composition of the Balıklı Spa water suggests that the origin of the thermal water is meteoric water.

  6. Different pathways of degradation of SP-A and saturated phosphatidylcholine by alveolar macrophages.

    PubMed

    Baritussio, A; Alberti, A; Armanini, D; Meloni, F; Bruttomesso, D

    2000-07-01

    Alveolar macrophages degrade surfactant protein (SP) A and saturated phosphatidycholine [dipalmitoylphosphatidylcholine (DPPC)]. To clarify this process, using rabbit alveolar macrophages, we analyzed the effect of drugs known to affect phagocytosis, pinocytosis, clathrin-mediated uptake, caveolae, the cytoskeleton, lysosomal pH, protein kinase C, and phosphatidylinositol 3-kinase (PI3K) on the degradation of SP-A and DPPC. We found the following: 1) SP-A binds to the plasma membrane, is rapidly internalized, and then moves toward degradative compartments. Uptake could be clathrin mediated, whereas phagocytosis, pinocytosis, or the use of caveolae are less likely. An intact cytoskeleton and an acidic milieu are necessary for the degradation of SP-A. 2) Stimulation of protein kinase C increases the degradation of SP-A. 3) PI3K influences the degradation of SP-A by regulating both the speed of internalization and subsequent intracellular steps, but its inhibition does not prevent SP-A from reaching the lysosomal compartment. 4) The degradation of DPPC is unaffected by most of the treatments able to influence the degradation of SP-A. Thus it appears that DPPC is degraded by alveolar macrophages through mechanisms very different from those utilized for the degradation of SP-A. PMID:10893207

  7. 77 FR 23109 - Airworthiness Directives; Agusta S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-18

    ...; 2. Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034, February...-17020; AD 2011-18-52] RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A. Helicopters AGENCY: Federal.... SUMMARY: We are publishing a new airworthiness directive (AD) for certain Agusta S.p.A. (Agusta)...

  8. 76 FR 60396 - Airworthiness Directives; Piaggio Aero Industries S.p.A. Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-29

    ... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... Industries S.p.A. Airplanes AGENCY: Federal Aviation Administration (FAA), Department of Transportation (DOT... (AD) for certain Piaggio Aero Industries S.p.A. Model P-180 airplanes. This proposed AD results...

  9. 78 FR 44042 - Airworthiness Directives; Agusta S.p.A. Helicopters (Type Certificate Currently Held By...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-23

    ...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); 3. Will not affect... TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A. Helicopters (Type Certificate Currently Held By AgustaWestland S.P.A) (AgustaWestland)...

  10. 76 FR 28169 - Airworthiness Directives; Agusta S.p.A. Model AB412 Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-16

    ... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or...-27-AD; Amendment 39-16692; AD 2011-10-11] RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A. Model... Agusta S.p.A. (Agusta) Model AB412 helicopters. This AD results from mandatory continuing...

  11. 78 FR 69600 - Airworthiness Directives; Piaggio Aero Industries S.p.A Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-20

    ... Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will not affect intrastate aviation in Alaska... Industries S.p.A Airplanes AGENCY: Federal Aviation Administration (FAA), Department of Transportation (DOT... (AD) for certain Piaggio Aero Industries S.p.A. Model P-180 airplanes. This proposed AD results...

  12. 77 FR 1654 - Airworthiness Directives; Agusta S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-11

    ...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); 3. Will not affect... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A...). SUMMARY: We propose to adopt a new airworthiness directive (AD) for all Agusta S.p.A. (Agusta) Model...

  13. Degradation of Arabidopsis CRY2 is regulated by SPA proteins and phytochrome A.

    PubMed

    Weidler, Guido; Zur Oven-Krockhaus, Sven; Heunemann, Michael; Orth, Christian; Schleifenbaum, Frank; Harter, Klaus; Hoecker, Ute; Batschauer, Alfred

    2012-06-01

    The UV-A/blue light photoreceptor crytochrome2 (cry2) plays a fundamental role in the transition from the vegetative to the reproductive phase in the facultative long-day plant Arabidopsis thaliana. The cry2 protein level strongly decreases when etiolated seedlings are exposed to blue light; cry2 is first phosphorylated, polyubiquitinated, and then degraded by the 26S proteasome. COP1 is involved in cry2 degradation, but several cop1 mutants show only reduced but not abolished cry2 degradation. SUPPRESSOR OF PHYA-105 (SPA) proteins are known to work in concert with COP1, and recently direct physical interaction between cry2 and SPA1 was demonstrated. Thus, we hypothesized that SPA proteins could also play a role in cry2 degradation. To this end, we analyzed cry2 protein levels in spa mutants. In all spa mutants analyzed, cry2 degradation under continuous blue light was alleviated in a fluence rate-dependent manner. Consistent with a role of SPA proteins in phytochrome A (phyA) signaling, a phyA mutant had enhanced cry2 levels, particularly under low fluence rate blue light. Fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy studies showed a robust physical interaction of cry2 with SPA1 in nuclei of living cells. Our results suggest that cry2 stability is controlled by SPA and phyA, thus providing more information on the molecular mechanisms of interaction between cryptochrome and phytochrome photoreceptors.

  14. 75 FR 65261 - Virginia Graeme Baker Pool and Spa Safety Act; Public Accommodation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-22

    ... accommodations facility'' on March 15, 2010 (75 FR 12167). The proposed interpretive rule would interpret... COMMISSION 16 CFR Part 1450 Virginia Graeme Baker Pool and Spa Safety Act; Public Accommodation AGENCY... ``public accommodations facility'' as used in the Virginia Graeme Baker Pool and Spa Safety Act....

  15. 75 FR 21985 - Virginia Graeme Baker Pool and Spa Safety Act; Interpretation of Unblockable Drain

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-27

    ... a notice in the Federal Register (74 FR 54301) announcing that it would be conducting a public... COMMISSION 16 CFR Part 1450 Virginia Graeme Baker Pool and Spa Safety Act; Interpretation of Unblockable... ``unblockable drain'' as used in the Virginia Graeme Baker Pool and Spa Safety Act (``VGB Act''). DATES:...

  16. 77 FR 52270 - Airworthiness Directives; Agusta S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-29

    ... Policies and Procedures (44 FR 11034, February 26, 1979); 3. Will not affect intrastate aviation in Alaska... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A...). SUMMARY: We propose to adopt a new airworthiness directive (AD) for the Agusta S.p.A. (Agusta) Model...

  17. 78 FR 49662 - Airworthiness Directives; PIAGGIO AERO INDUSTRIES S.p.A Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-15

    ... Industries S.p.A Airworthiness Office; Via Luigi Cibrario, 4-16154 Genova-Italy; telephone: +39 010 6481353.... 39.13 0 2. The FAA amends Sec. 39.13 by removing AD 99-07-10, Amendment 39- 11095 (64 FR 14824, March... INDUSTRIES S.p.A Airplanes AGENCY: Federal Aviation Administration (FAA), Department of Transportation...

  18. Blue-light-dependent interaction of cryptochrome 1 with SPA1 defines a dynamic signaling mechanism.

    PubMed

    Lian, Hong-Li; He, Sheng-Bo; Zhang, Yan-Chun; Zhu, Dan-Meng; Zhang, Jing-Yi; Jia, Kun-Peng; Sun, Shu-Xia; Li, Ling; Yang, Hong-Quan

    2011-05-15

    Cryptochromes (CRYs) are blue-light photoreceptors that mediate various light responses in plants and animals. The signaling mechanism by which CRYs regulate light responses involves their physical interactions with COP1. Here, we report that CRY1 interacts physically with SPA1 in a blue-light-dependent manner. SPA acts genetically downstream from CRYs to regulate light-controlled development. Blue-light activation of CRY1 attenuates the association of COP1 with SPA1 in both yeast and plant cells. These results indicate that the blue-light-triggered CRY1-SPA1 interaction may negatively regulate COP1, at least in part, by promoting the dissociation of COP1 from SPA1. This interaction and consequent dissociation define a dynamic photosensory signaling mechanism.

  19. Prevalence of Staphylococcus aureus protein A (spa) mutants in the community and hospitals in Oxfordshire

    PubMed Central

    2014-01-01

    Background Staphylococcal protein A (spa) is an important virulence factor which enables Staphylococcus aureus to evade host immune responses. Genotypes known as “spa-types”, based on highly variable Xr region sequences of the spa-gene, are frequently used to classify strains. A weakness of current spa-typing primers is that rearrangements in the IgG-binding region of the gene cause 1-2% of strains to be designated as “non-typeable”. Results We developed an improved primer which enabled sequencing of all strains, containing any type of genetic rearrangement, in a large study among community carriers and hospital inpatients in Oxfordshire, UK (6110 isolates). We identified eight novel spa-gene variants, plus one previously described. Three of these rearrangements would be designated “non-typeable” using current spa-typing methods; they occurred in 1.8% (72/3905) asymptomatically carried and 0.6% (14/2205) inpatient S. aureus strains. Some individuals were simultaneously colonized by both formerly non-typeable and typeable strains; previously such patients would have been identified as carrying only currently typeable strains, underestimating mixed carriage prevalence and diversity. Formerly non-typeable strains were found in more spa-types associated with multilocus sequence type ST398 (35%), common among livestock, compared to other groups with any non-typeable strains (1-4%), suggesting particular spa-types may have been under-represented in previous human studies. Conclusions This improved method allows us to spa-type previously non-typeable strains with rearrangements in the spa-gene and to resolve cases of mixed colonization with deletions in one or more strains, thus accounting for hidden diversity of S. aureus in both community and hospital environments. PMID:24621342

  20. Spa therapy for elderly: a retrospective study of 239 older patients with osteoarthritis

    NASA Astrophysics Data System (ADS)

    Karagülle, Mine; Kardeş, Sinan; Dişçi, Rian; Gürdal, Hatice; Karagülle, Müfit Zeki

    2016-10-01

    Very few studies tested the effectiveness of spa therapy in older patients with osteoarthritis. Therefore, we aimed to evaluate the short-term effects of spa therapy in patients aged 65 years and older with generalized, knee, hip, and cervical and lumbar spine osteoarthritis. In an observational retrospective study design at the Medical Ecology and Hydroclimatology Department of Istanbul Medical Faculty, we analyzed the records of 239 patients aged over 65 years with the diagnosis of all types of osteoarthritis who were prescribed a spa therapy course in some spa resorts in Turkey between 7 March 2002 and 31 December 2012. They travelled to a spa resort where they stayed at a thermal spa hotel and followed the usual therapy packages for 2 weeks. Patients were assessed by an experienced physician within a week before the spa journey and within a week after the completion of the spa therapy. Compared with baseline in whole sample, statistically significant improvements were observed in pain (visual analog scale, VAS), patient and physician global assessments (VAS), Health Assessment Questionnaire disability index (HAQ-DI), Lequesne algofunctional index (LAFI) for knee, Western Ontario and McMaster Universities index (WOMAC), Waddell disability index (WDI), and Neck Pain and Disability Scale (NPAD). According to Outcome Measures in Rheumatology—Osteoarthritis Research Society International (OMERACT-OARSI) Set of Responder Criteria, responder rate were 63.8 % (51/80) in generalized, 52 % (13/25) in knee, 50 % (2/4) in hip, 66.7 % (8/12) in lumbar, and 100 % (6/6) in cervical osteoarthritis subgroups. Spa therapy improved pain and physical functional status in older patients with osteoarthritis, especially generalized osteoarthritis and multiple joint osteoarthritis with involvement of knee. This improvement was clinically important in majority of the patients. To confirm the results of this preliminary study, there is a need of a randomized controlled clinical study

  1. Spa therapy for elderly: a retrospective study of 239 older patients with osteoarthritis

    NASA Astrophysics Data System (ADS)

    Karagülle, Mine; Kardeş, Sinan; Dişçi, Rian; Gürdal, Hatice; Karagülle, Müfit Zeki

    2016-01-01

    Very few studies tested the effectiveness of spa therapy in older patients with osteoarthritis. Therefore, we aimed to evaluate the short-term effects of spa therapy in patients aged 65 years and older with generalized, knee, hip, and cervical and lumbar spine osteoarthritis. In an observational retrospective study design at the Medical Ecology and Hydroclimatology Department of Istanbul Medical Faculty, we analyzed the records of 239 patients aged over 65 years with the diagnosis of all types of osteoarthritis who were prescribed a spa therapy course in some spa resorts in Turkey between 7 March 2002 and 31 December 2012. They travelled to a spa resort where they stayed at a thermal spa hotel and followed the usual therapy packages for 2 weeks. Patients were assessed by an experienced physician within a week before the spa journey and within a week after the completion of the spa therapy. Compared with baseline in whole sample, statistically significant improvements were observed in pain (visual analog scale, VAS), patient and physician global assessments (VAS), Health Assessment Questionnaire disability index (HAQ-DI), Lequesne algofunctional index (LAFI) for knee, Western Ontario and McMaster Universities index (WOMAC), Waddell disability index (WDI), and Neck Pain and Disability Scale (NPAD). According to Outcome Measures in Rheumatology—Osteoarthritis Research Society International (OMERACT-OARSI) Set of Responder Criteria, responder rate were 63.8 % (51/80) in generalized, 52 % (13/25) in knee, 50 % (2/4) in hip, 66.7 % (8/12) in lumbar, and 100 % (6/6) in cervical osteoarthritis subgroups. Spa therapy improved pain and physical functional status in older patients with osteoarthritis, especially generalized osteoarthritis and multiple joint osteoarthritis with involvement of knee. This improvement was clinically important in majority of the patients. To confirm the results of this preliminary study, there is a need of a randomized controlled clinical study

  2. A randomized controlled clinical trial of SPA -- the Seattle Protocol for Activity in older adults

    PubMed Central

    Teri, Linda; McCurry, Susan M.; Logsdon, Rebecca G.; Gibbons, Laura E.; Buchner, David M.; Larson, Eric B.

    2012-01-01

    OBJECTIVES Evaluate the efficacy of a physical activity program (Seattle Protocol for Activity: SPA) for low-exercising older adults, compared to educational health promotion program (HP), combination treatment (SPA+HP), and routine medical care control conditions (RMC). DESIGN Single-blinded, randomized controlled trial with 2 × 2 factorial design. SETTING: November 2001 to September 2004, in community centers in King County, Washington. PARTICIPANTS 273 community-residing, cognitively intact older adults (mean age, 79.2 y; 62% women). INTERVENTIONS SPA (in-class exercises with assistance setting weekly home exercise goals), and HP (information about age-appropriate topics relevant to enhancing health), with randomization to four conditions: SPA only (n = 69), HP only (n = 73), SPA+HP (n = 67), and RMC control (n = 64). Active treatment participants attended nine group classes over three months, followed by five booster sessions over one year. MAIN OUTCOME MEASURES Self-rated health (SF-36) and depression (GDS). Secondary ratings of physical performance, treatment adherence, and self-rated health and affective function were also collected. RESULTS At 3-months, participants in SPA exercised more and had significantly better self-reported health, strength, and general well-being (p<.05) than participants in HP or RMC. Over 18 months, SPA participants maintained health and physical function benefits, and had continued to exercise more than non-SPA participants. SPA+HP was not significantly better than SPA alone. Better adherence was associated with better outcomes. CONCLUSION Older adults participating in low levels of regular exercise can establish and maintain a home-based exercise program that yields immediate and long-term physical and affective benefits. PMID:21718259

  3. Comparing whole-genome sequencing with Sanger sequencing for spa typing of methicillin-resistant Staphylococcus aureus.

    PubMed

    Bartels, Mette Damkjær; Petersen, Andreas; Worning, Peder; Nielsen, Jesper Boye; Larner-Svensson, Hanna; Johansen, Helle Krogh; Andersen, Leif Percival; Jarløv, Jens Otto; Boye, Kit; Larsen, Anders Rhod; Westh, Henrik

    2014-12-01

    spa typing of methicillin-resistant Staphylococcus aureus (MRSA) has traditionally been done by PCR amplification and Sanger sequencing of the spa repeat region. At Hvidovre Hospital, Denmark, whole-genome sequencing (WGS) of all MRSA isolates has been performed routinely since January 2013, and an in-house analysis pipeline determines the spa types. Due to national surveillance, all MRSA isolates are sent to Statens Serum Institut, where the spa type is determined by PCR and Sanger sequencing. The purpose of this study was to evaluate the reliability of the spa types obtained by 150-bp paired-end Illumina WGS. MRSA isolates from new MRSA patients in 2013 (n = 699) in the capital region of Denmark were included. We found a 97% agreement between spa types obtained by the two methods. All isolates achieved a spa type by both methods. Nineteen isolates differed in spa types by the two methods, in most cases due to the lack of 24-bp repeats in the whole-genome-sequenced isolates. These related but incorrect spa types should have no consequence in outbreak investigations, since all epidemiologically linked isolates, regardless of spa type, will be included in the single nucleotide polymorphism (SNP) analysis. This will reveal the close relatedness of the spa types. In conclusion, our data show that WGS is a reliable method to determine the spa type of MRSA.

  4. 78 FR 16506 - Notice of Hearing: Reconsideration of Disapproval of Florida State Plan Amendments (SPA) 12-015

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-15

    ... Disapproval of Florida State Plan Amendments (SPA) 12-015 AGENCY: Centers for Medicare & Medicaid Services... Florida SPA 12- 015. DATES: Closing Date: Requests to participate in the hearing as a party must be... reconsider CMS' decision to disapprove Florida SPA 12-015 which was submitted on September 14, 2012,...

  5. Comparing whole-genome sequencing with Sanger sequencing for spa typing of methicillin-resistant Staphylococcus aureus.

    PubMed

    Bartels, Mette Damkjær; Petersen, Andreas; Worning, Peder; Nielsen, Jesper Boye; Larner-Svensson, Hanna; Johansen, Helle Krogh; Andersen, Leif Percival; Jarløv, Jens Otto; Boye, Kit; Larsen, Anders Rhod; Westh, Henrik

    2014-12-01

    spa typing of methicillin-resistant Staphylococcus aureus (MRSA) has traditionally been done by PCR amplification and Sanger sequencing of the spa repeat region. At Hvidovre Hospital, Denmark, whole-genome sequencing (WGS) of all MRSA isolates has been performed routinely since January 2013, and an in-house analysis pipeline determines the spa types. Due to national surveillance, all MRSA isolates are sent to Statens Serum Institut, where the spa type is determined by PCR and Sanger sequencing. The purpose of this study was to evaluate the reliability of the spa types obtained by 150-bp paired-end Illumina WGS. MRSA isolates from new MRSA patients in 2013 (n = 699) in the capital region of Denmark were included. We found a 97% agreement between spa types obtained by the two methods. All isolates achieved a spa type by both methods. Nineteen isolates differed in spa types by the two methods, in most cases due to the lack of 24-bp repeats in the whole-genome-sequenced isolates. These related but incorrect spa types should have no consequence in outbreak investigations, since all epidemiologically linked isolates, regardless of spa type, will be included in the single nucleotide polymorphism (SNP) analysis. This will reveal the close relatedness of the spa types. In conclusion, our data show that WGS is a reliable method to determine the spa type of MRSA. PMID:25297335

  6. [INDENA SPA company's patent portfolio of Ginkgo biloba preparation].

    PubMed

    Wang, Nan; Guo, Kai; Cheng, Xin-min; Liu, Wei

    2015-10-01

    INDENA SPA Company in Italy is a multi-national company that produces and sells plant extracts. Based on its own re- search advantages in the field of Ginkgo biloba preparation, the company protects its own products market effectively through building patent portfolio around the patents of its opponent. Based on the multi-angle analysis for patent portfolio of G. biloba preparation from the aspects of application time, legal status, technical development route, and patent portfolio layout, this article provides technical reference on research and development of G. biloba preparation, and the author suggest that Chinese applicants learn techniques and layout experiences of other patents fully to enhance the level of research and patent protection level. PMID:26975120

  7. [INDENA SPA company's patent portfolio of Ginkgo biloba preparation].

    PubMed

    Wang, Nan; Guo, Kai; Cheng, Xin-min; Liu, Wei

    2015-10-01

    INDENA SPA Company in Italy is a multi-national company that produces and sells plant extracts. Based on its own re- search advantages in the field of Ginkgo biloba preparation, the company protects its own products market effectively through building patent portfolio around the patents of its opponent. Based on the multi-angle analysis for patent portfolio of G. biloba preparation from the aspects of application time, legal status, technical development route, and patent portfolio layout, this article provides technical reference on research and development of G. biloba preparation, and the author suggest that Chinese applicants learn techniques and layout experiences of other patents fully to enhance the level of research and patent protection level.

  8. Investigation of Spa Pools Associated with Lung Disorders Caused by Mycobacterium avium Complex in Immunocompetent Adults

    PubMed Central

    Lumb, Richard; Stapledon, Richard; Scroop, Andrew; Bond, Peter; Cunliffe, David; Goodwin, Allan; Doyle, Robyn; Bastian, Ivan

    2004-01-01

    Three cases of Mycobacterium avium complex-related lung disorders were associated with two poorly maintained spa pools by genotypic investigations. Inadequate disinfection of the two spas had reduced the load of environmental bacteria to less than 1 CFU/ml but allowed levels of M. avium complex of 4.3 × 104 and 4.5 × 103 CFU/ml. Persistence of the disease-associated genotype was demonstrated in one spa pool for over 5 months until repeated treatments with greater than 10 mg of chlorine per liter for 1-h intervals eliminated M. avium complex from the spa pool. A fourth case of Mycobacterium avium complex-related lung disease was associated epidemiologically but not genotypically with another spa pool that had had no maintenance undertaken. This spa pool contained low numbers of mycobacteria by smear and was culture positive for M. avium complex, and the nonmycobacterial organism count was 5.2 × 106 CFU/ml. Public awareness about the proper maintenance of private (residential) spa pools must be promoted by health departments in partnership with spa pool retailers. PMID:15294830

  9. Moon - 2 Views of Orientale Basin

    NASA Technical Reports Server (NTRS)

    1990-01-01

    These pictures of the Moon were taken by the Galileo spacecraft at (right photo) 6:47 p.m. PST Dec.8, 1990 from a distance of almost 220,000 miles, and at (left photo) 9:35 a.m. PST Dec. 9, 1990 at a range of more than 350,000 miles. The picture on the right shows the dark Oceanus Procellarum in the upper center, with Mare Imbrium above it and the smaller circular Mare Humorum below. The Orientale Basin, with a small mare in its center, is on the lower left near the limb or edge. Between stretches the cratered highland terrain, with scattered bright young craters on highlands and maria alike. The picture at left shows the globe of the Moon rotated, putting Mare Imbrium on the eastern limb and moving the Orientale Basin almost to the center. The extent of the cratered highlands on the far side is very apparent. At lower left, near the limb, is the South Pole Aitken basin, similar to Orientale but very much older and some 1,200 miles in diameter. This feature was previously known as a large depression in the southern far side; this image shows its Orientale like structure and darkness relative to surrounding highlands.

  10. A new silver based composite material for SPA water disinfection.

    PubMed

    Tartanson, M A; Soussan, L; Rivallin, M; Chis, C; Penaranda, D; Lapergue, R; Calmels, P; Faur, C

    2014-10-15

    A new composite material based on alumina (Al2O3) modified by two surface nanocoatings - titanium dioxide (TiO2) and silver (Ag) - was studied for spa water disinfection. Regarding the most common microorganisms in bathing waters, two non-pathogenic bacteria Escherichia coli (Gram-negative) and Staphylococcus epidermidis (Gram positive) were selected as surrogates for bacterial contamination. The bactericidal properties of the Al2O3-TiO2-Ag material were demonstrated under various operating conditions encountered in spa water (temperature: 22-37 °C, presence of salt: CaCO3 or CaCl2, high oxygen content, etc.). Total removal of 10(8) CFU mL(-1) of bacteria was obtained in less than 10 min with 16 g L(-1) of material. Best results were observed for both conditions: a temperature of 37 °C and under aerobic condition; this latest favouring Reactive Oxygen Species (ROS) generation. The CaCO3 salt had no impact on the bactericidal activity of the composite material and CaCl2 considerably stabilized the silver desorption from the material surface thanks to the formation of AgCl precipitate. Preliminary tests of the Al2O3-TiO2-Ag bactericidal behaviour in a continuous water flow confirmed that 2 g L(-1) of material eliminated more than 90% of a 2.0 × 10(8) CFU mL(-1) bacterial mixture after one water treatment recycle and reached the disinfection standard recommended by EPA (coliform removal = 6 log) within 22 h.

  11. A Small Conserved Domain in the Yeast Spa2p Is Necessary and Sufficient for Its Polarized Localization

    PubMed Central

    Arkowitz, Robert A.; Lowe, Nick

    1997-01-01

    SPA2 encodes a yeast protein that is one of the first proteins to localize to sites of polarized growth, such as the shmoo tip and the incipient bud. The dynamics and requirements for Spa2p localization in living cells are examined using Spa2p green fluorescent protein fusions. Spa2p localizes to one edge of unbudded cells and subsequently is observable in the bud tip. Finally, during cytokinesis Spa2p is present as a ring at the mother–daughter bud neck. The bud emergence mutants bem1 and bem2 and mutants defective in the septins do not affect Spa2p localization to the bud tip. Strikingly, a small domain of Spa2p comprised of 150 amino acids is necessary and sufficient for localization to sites of polarized growth. This localization domain and the amino terminus of Spa2p are essential for its function in mating. Searching the yeast genome database revealed a previously uncharacterized protein which we name, Sph1p (Spa2p homolog), with significant homology to the localization domain and amino terminus of Spa2p. This protein also localizes to sites of polarized growth in budding and mating cells. SPH1, which is similar to SPA2, is required for bipolar budding and plays a role in shmoo formation. Overexpression of either Spa2p or Sph1p can block the localization of either protein fused to green fluorescent protein, suggesting that both Spa2p and Sph1p bind to and are localized by the same component. The identification of a 150–amino acid domain necessary and sufficient for localization of Spa2p to sites of polarized growth and the existence of this domain in another yeast protein Sph1p suggest that the early localization of these proteins may be mediated by a receptor that recognizes this small domain. PMID:9214378

  12. Lunar impact basins and crustal heterogeneity: new Western limb and far side data from galileo.

    PubMed

    Belton, M J; Head, J W; Pieters, C M; Greeley, R; McEwen, A S; Neukum, G; Klaasen, K P; Anger, C D; Carr, M H; Chapman, C R; Davies, M E; Fanale, F P; Gierasch, P J; Greenberg, R; Ingersoll, A P; Johnson, T; Paczkowski, B; Pilcher, C B; Veverka, J

    1992-01-31

    Multispectral images of the lunar western limb and far side obtained from Galileo reveal the compositional nature of several prominent lunar features and provide new information on lunar evolution. The data reveal that the ejecta from the Orientale impact basin (900 kilometers in diameter) lying outside the Cordillera Mountains was excavated from the crust, not the mantle, and covers pre-Orientale terrain that consisted of both highland materials and relatively large expanses of ancient mare basalts. The inside of the far side South Pole-Aitken basin (>2000 kilometers in diameter) has low albedo, red color, and a relatively high abundance of iron- and magnesium-rich materials. These features suggest that the impact may have penetrated into the deep crust or lunar mantle or that the basin contains ancient mare basalts that were later covered by highlands ejecta.

  13. Lunar impact basins and crustal heterogeneity - New western limb and far side data from Galileo

    NASA Technical Reports Server (NTRS)

    Belton, Michael J. S.; Head, James W., III; Pieters, Carle M.; Greeley, Ronald; Mcewen, Alfred S.; Neukum, Gerhard; Klaasen, Kenneth P.; Anger, Clifford D.; Carr, Michael H.; Chapman, Clark R.

    1992-01-01

    Multispectral images of the lunar western limb and far side obtained from Galileo reveal the compositional nature of several prominent lunar features and provide new information on lunar evolution. The data reveal that the ejecta from the Orientale impact basin (900 kilometers in diameter) lying outside the Cordillera Mountains was excavated from the crust, not the mantle, and covers pre-Orientale terrain that consisted of both highland materials and relatively large expanses of ancient mare basalts. The inside of the far side South Pole-Aitken basin (greater than 2000 kilometers in diameter) has low albedo, red color, and a relatively high abundance of iron- and magnesium-rich materials. These features suggest that the impact may have penetrated into the deep crust or lunar mantle or that the basin contains ancient mare basalts that were later covered by highlands ejecta.

  14. Lunar impact basins and crustal heterogeneity: New western limb and far side data from galileo

    USGS Publications Warehouse

    Belton, M.J.S.; Head, J. W.; Pieters, C.M.; Greeley, R.; McEwen, A.S.; Neukum, G.; Klaasen, K.P.; Anger, C.D.; Carr, M.H.; Chapman, C.R.; Davies, M.E.; Fanale, F.P.; Gierasch, P.J.; Greenberg, R.; Ingersoll, A.P.; Johnson, T.; Paczkowski, B.; Pilcher, C.B.; Veverka, J.

    1992-01-01

    Multispectral images of the lunar western limb and far side obtained from Galileo reveal the compositional nature of several prominent lunar features and provide new information on lunar evolution. The data reveal that the ejecta from the Orientale impact basin (900 kilometers in diameter) lying outside the Cordillera Mountains was excavated from the crust, not the mantle, and covers pre-Orientale terrain that consisted of both highland materials and relatively large expanses of ancient mare basalts. The inside of the far side South Pole-Aitken basin (>2000 kilometers in diameter) has low albedo, red color, and a relatively high abundance of iron- and magnesium-rich materials. These features suggest that the impact may have penetrated into the deep crust or lunar mantle or that the basin contains ancient mare basalts that were later covered by highlands ejecta.

  15. A Large Basin on the Near Side of the Moon

    NASA Astrophysics Data System (ADS)

    Byrne, Charles Joseph

    2007-12-01

    The differences between the surface structure of the near side and the far side of the Moon have been topics of interest ever since photographs of the far side have been available. One recurrent hypothesis is that a large impact on the near side has deposited ejecta on the far side, resulting in thicker crust there. Specific proposals were made by P.H. Cadogan for the Gargantuan Basin and by E.A. Whitaker for the Procellarum Basin. Despite considerable effort, no consensus has been reached on the existence of these basins. The problem of searching for such a basin is one of finding its signature in a somewhat chaotic field of basin and crater impacts. The search requires a model of the topographic shape of an impact basin and its ejecta field. Such a model is described, based on elevation data of lunar basins collected by the Lidar instrument of the Clementine mission and crustal thickness data derived from tracking Clementine and other spacecraft. The parameters of the model are scaled according to the principles of dimensional analysis and isostatic compensation in the early Moon. The orbital dynamics of the ejecta and the curvature of the Moon are also taken into account. Using such a scaled model, a search for the best fit for a large basin led to identification of a basin whose cavity covers more than half the Moon, including the area of all of the impact basins visible on the near side. The center of this basin is at 22 degrees east longitude and 8.5 degrees north latitude and its average radius is approximately 3,160 km. It is a megabasin, a basin that contains other basins (the far side South Pole-Aitken Basin also qualifies for that designation). It has been called the Near Side Megabasin. Much of the material ejected from the basin escaped the Moon, but the remainder formed an ejecta blanket that covered all of the far side beyond the basin rim to a depth of from 6 to 30 km. Isostatic compensation reduced the depth relative to the mean surface to a range

  16. COP1/SPA ubiquitin ligase complexes repress anthocyanin accumulation under low light and high light conditions.

    PubMed

    Maier, Alexander; Hoecker, Ute

    2015-01-01

    In Arabidopsis and many other plant species, anthocyanin pigments accumulate only after light exposure and not in darkness. Excess light of very high fluence rates leads to a further, very strong increase in anthocyanin levels. How excess light is sensed is not well understood. Here, we show that mutations in the key repressor of light signaling, the COP1/SPA complex, cause a strong hyperaccumulation of anthocyanins not only under normal light but also under excess, high light conditions. Hence, normal light signaling via COP1/SPA is required to prevent hyperaccumulation of anthocyanins under these high light conditions. However, since cop1 and spa mutants show a similar high-light responsiveness of anthocyanin accumulation as the wild type it remains to be resolved whether COP1/SPA is directly involved in the high-light response itself.

  17. RADON AND PROGENY SOURCED DOSE ASSESSMENT OF SPA EMPLOYEES IN BALNEOLOGICAL SITES.

    PubMed

    Uzun, Sefa Kemal; Demiröz, Işık

    2016-09-01

    This study was conducted in the scope of IAEA project with the name 'Establishing a Systematic Radioactivity Survey and Total Effective Dose Assessment in Natural Balneological Sites' (TUR/9/018), at the Health Physics department of Sarayköy Nuclear Research and Training Center (SANAEM). The aim of this study is estimation of radon and progeny sourced effective dose for the people who are working at the spa facilities by measuring radon activity concentration (RAC) at the ambient air of indoor spa pools and dressing rooms. As it is known, the source of the radon gas is the radium content of the earth crust. Therefore, thermal waters coming from ground may contain dissolved radon and the radon can diffuse water to air. So the ambient air of spa pools can contain serious RAC that depends on a lot of parameters. In this regard, RAC measurements were executed at the 70 spa facilities in Turkey. The measurements were done with both active and passive methods at ambient air of spa pools and dressing rooms. Thus, active measurements were carried out by using the Alphaguard(®) with diffusion mode during half an hour, and passive measurements were carried out by using the humidity resistive CR-39 radon detectors during 2 months. Results show that RAC values at ambient air of spa pools varies between 13 Bq m(-3) and 10 kBq m(-3) Because long-term measurements are more reliable, if it is available, for dose calculations passive radon measurements (with CR-39 detectors) at ambient air of spa pools and dressing rooms were used, otherwise active measurement results were used. With the measurement by the conversion coefficients of ICRP 65 and occupational data of the employees has got from questionary forms, effective dose values were calculated. According to the calculations, spa employees are exposed to annual average dose between 0.05 and 29 mSv because of radon and progeny.

  18. RADON AND PROGENY SOURCED DOSE ASSESSMENT OF SPA EMPLOYEES IN BALNEOLOGICAL SITES.

    PubMed

    Uzun, Sefa Kemal; Demiröz, Işık

    2016-09-01

    This study was conducted in the scope of IAEA project with the name 'Establishing a Systematic Radioactivity Survey and Total Effective Dose Assessment in Natural Balneological Sites' (TUR/9/018), at the Health Physics department of Sarayköy Nuclear Research and Training Center (SANAEM). The aim of this study is estimation of radon and progeny sourced effective dose for the people who are working at the spa facilities by measuring radon activity concentration (RAC) at the ambient air of indoor spa pools and dressing rooms. As it is known, the source of the radon gas is the radium content of the earth crust. Therefore, thermal waters coming from ground may contain dissolved radon and the radon can diffuse water to air. So the ambient air of spa pools can contain serious RAC that depends on a lot of parameters. In this regard, RAC measurements were executed at the 70 spa facilities in Turkey. The measurements were done with both active and passive methods at ambient air of spa pools and dressing rooms. Thus, active measurements were carried out by using the Alphaguard(®) with diffusion mode during half an hour, and passive measurements were carried out by using the humidity resistive CR-39 radon detectors during 2 months. Results show that RAC values at ambient air of spa pools varies between 13 Bq m(-3) and 10 kBq m(-3) Because long-term measurements are more reliable, if it is available, for dose calculations passive radon measurements (with CR-39 detectors) at ambient air of spa pools and dressing rooms were used, otherwise active measurement results were used. With the measurement by the conversion coefficients of ICRP 65 and occupational data of the employees has got from questionary forms, effective dose values were calculated. According to the calculations, spa employees are exposed to annual average dose between 0.05 and 29 mSv because of radon and progeny. PMID:26424134

  19. In vitro activity of a new polyene, SPA-S-843, against yeasts.

    PubMed

    Rimaroli, C; Bruzzese, T

    1998-11-01

    The in vitro activity of a new water-soluble polyene, SPA-S-843, was evaluated against 116 strains of Candida, Cryptococcus, and Saccharomyces spp. and compared with that of amphotericin B. SPA-S-843 demonstrated better inhibitory activity against all of the yeasts examined and better fungicidal activity against Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis than did amphotericin B.

  20. SP-A and SP-D in host defense against fungal infections and allergies.

    PubMed

    Pandit, Hrishikesh; Madhukaran, Shanmuga P; Nayak, Annapurna; Madan, Taruna

    2012-01-01

    Innate immunity mediated by pattern recognition proteins is relevant in the host defense against fungi. SP-A and SP-D are two such proteins belonging to the class of collagen domain containing C-type lectins, or collectins. They bind to the sugar moieties present on the cell walls of various fungi in a dose dependent manner via their carbohydrate recognition domain (CRD). SP-A and SP-D directly interact with alveolar macrophages, neutrophils, lymphocytes. We review these roles of SP-A and SP-D against various clinically relevant fungal pathogens and fungal allergens. SP-A and SP-D gene deficient mice showed increased susceptibility/ resistance to various fungal infections. Patients of fungal infections and allergies are reported with alterations in the serum or lung lavage levels of SP-A and SP-D. There are studies associating the gene polymorphisms in SP-A and SP-D with alterations in their levels or functions or susceptibility of the host to fungal diseases. In view of the protective role of SP-D in murine models of Aspergillus fumigatus infections and allergies, therapeutic use of SP-D could be explored further.

  1. Spa treatment (balneotherapy) for fibromyalgia-a qualitative-narrative review and a historical perspective.

    PubMed

    Ablin, Jacob N; Häuser, Winfried; Buskila, Dan

    2013-01-01

    Aim. To perform a narrative review of spa therapy for management of the fibromyalgia syndrome (FMS), evaluating this traditional time-honored form of therapy in a historical perspective. Methods. Medline was searched using the terms "Spa therapy," "Balneotherapy," and "Fibromyalgia" between 1990 (year of ACR fibromyalgia criteria publication) and April 2013. The Cochrane database was also searched. Publications relating to the implementation of spa therapy and related practices over the centuries were identified through references, searched, and reviewed. Results. Reports of balneotherapy were described from diverse locations throughout Europe and Asia, and various forms of water-related therapy have been incorporated for many musculoskeletal indications. In the management of FMS, spa therapy has generally been shown to be well accepted and moderately effective for symptom reduction. Conclusion. While achieving high-quality evidence-based conclusions is difficult for complex natural therapies such as spa therapy, the existing evidence indicates a positive effect in management of FMS. In view of the long history of this modality in the management of rheumatic pain as well as the inherent difficulties related to pharmacological treatment, the role of spa therapy should currently be recognized as part of a therapeutic program for FMS.

  2. Spa Treatment (Balneotherapy) for Fibromyalgia—A Qualitative-Narrative Review and a Historical Perspective

    PubMed Central

    Ablin, Jacob N.; Buskila, Dan

    2013-01-01

    Aim. To perform a narrative review of spa therapy for management of the fibromyalgia syndrome (FMS), evaluating this traditional time-honored form of therapy in a historical perspective. Methods. Medline was searched using the terms “Spa therapy,” “Balneotherapy,” and “Fibromyalgia” between 1990 (year of ACR fibromyalgia criteria publication) and April 2013. The Cochrane database was also searched. Publications relating to the implementation of spa therapy and related practices over the centuries were identified through references, searched, and reviewed. Results. Reports of balneotherapy were described from diverse locations throughout Europe and Asia, and various forms of water-related therapy have been incorporated for many musculoskeletal indications. In the management of FMS, spa therapy has generally been shown to be well accepted and moderately effective for symptom reduction. Conclusion. While achieving high-quality evidence-based conclusions is difficult for complex natural therapies such as spa therapy, the existing evidence indicates a positive effect in management of FMS. In view of the long history of this modality in the management of rheumatic pain as well as the inherent difficulties related to pharmacological treatment, the role of spa therapy should currently be recognized as part of a therapeutic program for FMS. PMID:23983795

  3. SPA Meteor Section results: September-October 2001

    NASA Astrophysics Data System (ADS)

    McBeath, A.

    2002-10-01

    Summaries of reports and correspondence received by the SPA Meteor Section form September and October, 2001 are given. September was disappointing visually, while in the radio data Sporadic-E continued to be problematical from the main May-August spell, along with difficulties because of declining transmitter numbers, especially for European observers. A discussion of the supposedly new minor shower, the so-called "September Taurids" (O'Meara 2002), in connection with radio results back to 1989, is also provided. In October, the Orionids enjoyed moon-less conditions, and were seen to peak on October 21 (mean ZHR=19+-5). The radio observations showed little consensus as to whether October 20 or 21 produced the higher echo counts however, and no specific timing could be defined beyond this. With strong aurorae present in some radio and visual data (unusually seen down to southern England on October 21-22), such difficulties were perhaps unavoidable. Radio data picked up a weak maximum around lambda=214-215 (October 27-28) which has been seen only occasionally before. The same night provided observers around on the North Sea with a spectacular brilliant green bolide at 19h20m20s UT. -------------------------cut here ------------------------------

  4. SPA Meteor Section Results: October-December 2004

    NASA Astrophysics Data System (ADS)

    McBeath, Alastair

    2007-08-01

    Notes and details extracted from SPA Meteor Section analyses are given with some discussion. No radio Draconid signature was apparent on either October 5 or 8, but the Orionids received useful visual and radio coverage, the shower producing fine activity from October 20 to 24 or so, with a probable peak on October 21/22, when ZHRs were approximately 24+-2. The early Leonid peak predicted for November 8/9 was not found in the radio data, though healthy Leonid activity was caught visually and by radio from November 17 to 21 or 22. A ZHR peak was calculated for November 19, at apptoximately 26+-2. The brief, moderately strong Leonid maximum computed from IMO results on November 17 at 21h UT, was also detected by one radio system from Japan. One UK fireball of magnitude -4/-6 was seen well enough for an approximate atmospheric trajectory to be established, at 07h05m UT on November 29. In December, aside from the Ursid results already described, the Geminids were well-seen visually, and by the radio observers. A Geminid peak around approximately 22h+-1h UT on December 13 (solar longitude (eq. 2000.0) 262.19 deg +- 0.04 deg), was suggested from the radio findings.

  5. Surfactant protein A (SP-A) inhibits agglomeration and macrophage uptake of toxic amine modified nanoparticles

    PubMed Central

    McKenzie, Zofi; Kendall, Michaela; Mackay, Rose-Marie; Whitwell, Harry; Elgy, Christine; Ding, Ping; Mahajan, Sumeet; Morgan, Cliff; Griffiths, Mark; Clark, Howard; Madsen, Jens

    2015-01-01

    Abstract The lung provides the main route for nanomaterial exposure. Surfactant protein A (SP-A) is an important respiratory innate immune molecule with the ability to bind or opsonise pathogens to enhance phagocytic removal from the airways. We hypothesised that SP-A, like surfactant protein D, may interact with inhaled nanoparticulates, and that this interaction will be affected by nanoparticle (NP) surface characteristics. In this study, we characterise the interaction of SP-A with unmodified (U-PS) and amine-modified (A-PS) polystyrene particles of varying size and zeta potential using dynamic light scatter analysis. SP-A associated with both 100 nm U-PS and A-PS in a calcium-independent manner. SP-A induced significant calcium-dependent agglomeration of 100 nm U-PS NPs but resulted in calcium-independent inhibition of A-PS self agglomeration. SP-A enhanced uptake of 100 nm U-PS into macrophage-like RAW264.7 cells in a dose-dependent manner but in contrast inhibited A-PS uptake. Reduced association of A-PS particles in RAW264.7 cells following pre-incubation of SP-A was also observed with coherent anti-Stokes Raman spectroscopy. Consistent with these findings, alveolar macrophages (AMs) from SP-A−/− mice were more efficient at uptake of 100 nm A-PS compared with wild type C57Bl/6 macrophages. No difference in uptake was observed with 500 nm U-PS or A-PS particles. Pre-incubation with SP-A resulted in a significant decrease in uptake of 100 nm A-PS in macrophages isolated from both groups of mice. In contrast, increased uptake by AMs of U-PS was observed after pre-incubation with SP-A. Thus we have demonstrated that SP-A promotes uptake of non-toxic U-PS particles but inhibits the clearance of potentially toxic A-PS particles by blocking uptake into macrophages. PMID:25676620

  6. Treatment with mPEG-SPA improves the survival of corneal grafts in rats by immune camouflage.

    PubMed

    Wang, Shuangyong; Li, Liangliang; Liu, Ying; Li, Chaoyang; Zhang, Min; Wang, Bowen; Huang, Zheqian; Gao, Xinbo; Wang, Zhichong

    2015-03-01

    We investigated the immune camouflage effects of methoxy polyethylene glycol succinimidyl propionate (mPEG-SPA) on corneal antigens and explored a novel approach for reducing corneal antigenicity, thereby decreasing corneal graft rejection. Importantly, this approach did not alter normal local immunity. Corneal grafts were treated with mPEG-SPA 5KD or 20KD (3% W/V), which could shield major histocompatibility antigen class I molecules (RT1-A) of corneal grafts. Skin grafts of Wistar rats were transplanted to SD rats. Then the splenic lymphocytes were isolated from SD rats. Subsequently, the lymphocytes were co-cultured with autologous corneal grafts or untreated corneal grafts and PEGylated grafts treated with mPEG-SPA 5KD or 20KD obtained from the counterpart skin donors, which were used as autologous control, allogeneic control, mPEG-SPA 5KD group and mPEG-SPA 20KD group, respectively. Lymphocyte proliferation was lower in mPEG-SPA 5KD group and mPEG-SPA 20KD group than in the allogeneic control. SD rats with corneal neovascularisation were used as recipients for high-risk corneal transplantation and were randomly divided into four groups: autologous control, allogeneic control, mPEG-SPA 5KD group and mPEG-SPA 20KD group. The recipients received corneal grafts from Wistar rats. Corneal graft survival was prolonged and graft rejection was reduced in the mPEG-SPA 5KD group and the mPEG-SPA 20KD group compared to the allogeneic control. Thus, we think that mPEG-SPA could immunologically camouflage corneal antigens to prolong corneal grafts survival in high-risk transplantation.

  7. Effect of edaravone on serum SP-A and arterial blood gas in patients with lobectomy

    PubMed Central

    Song, Li; Xiong, Feng; Zhang, Xue-Juan; Liu, Wei-Yi; Zhao, Yang; Feng, Wei

    2015-01-01

    Objective: To discuss the effect of edaravone on serum pulmonary surfactant protein A (SP-A) and arterial blood gas (ABG) in patients with thoracoscopic lobectomy. Methods: 40 lung cancer patients with right side of lobectomy were randomly divided into control group (group C, 20 cases) and edaravone group (group E, 20 cases). Group E was treated edaravone (1 mg/kg) between induction and skin incision, dropping within 30 min; group C was treated with equivalent normal saline. The venous and arterial blood were collected in both groups immediately before incision (T0), after 1 h of one-lung ventilation 1 h (T1) and in 1 h after lungs ventilation (T2) for ABG analysis and measurement of serum SP-A level. Results After OLV, serum SP-A levels were significantly increased in both groups (P < 0.05); compared with group C, serum levels of SP-A were reduced (P < 0.05) and ABG was significantly improved in group E. Conclusion: Edaravone can reduce serum SP-A levels in patients with lobectomy and alleviate acute lung injury to a certain extent in surgery. PMID:26309693

  8. Sex differences in the acute in vivo effects of different human SP-A variants on the mouse alveolar macrophage proteome

    PubMed Central

    Phelps, David S.; Umstead, Todd M.; Floros, Joanna

    2014-01-01

    Surfactant protein A (SP-A) is involved in lung innate immunity. Humans have two SP-A genes, SFTPA1 and SFTPA2, each with several variants. We examined the in vivo effects of treatment with specific SP-A variants on the alveolar macrophage (AM) proteome from SP-A knockout (KO) mice. KO mice received either SP-A1, SP-A2, or both. AM were collected and their proteomes examined with 2D-DIGE. We identified 90 proteins and categorized them as related to actin/cytoskeleton, oxidative stress, protease balance/chaperones, regulation of inflammation, and regulatory/developmental processes. SP-A1 and SP-A2 had different effects on the AM proteome and these effects differed between sexes. In males more changes occurred in the oxidative stress, protease/chaperones, and inflammation groups with SP-A2 treatment than with SP-A1. In females most SP-A1-induced changes were in the actin/cytoskeletal and oxidative stress groups. We conclude that after acute SP-A1 and SP-A2 treatment, sex-specific differences were observed in the AM proteomes from KO mice, and that these sex differences differ in response to SP-A1 and SP-A2. Females are more responsive to SP-A1, whereas the gene-specific differences in males were minimal. These observations not only demonstrate the therapeutic potential of exogenous SP-A, but also illustrate sex- and gene-specific differences in the response to it. PMID:24954098

  9. Ancient multiring basins on the moon revealed by clementine laser altimetry.

    PubMed

    Spudis, P D; Gillis, J J; Reisse, R A

    1994-12-16

    Analysis of laser altimetry data from Clementine has confirmed and extended our knowledge of nearly obliterated multiring basins on the moon. These basins were formed during the early bombardment phase of lunar history, have been filled to varying degrees by mare lavas and regional ejecta blankets, and have been degraded by the superposition of large impact craters. The Mendel-Rydberg Basin, a degraded three-ring feature over 600 kilometers in diameter on the lunar western limb, is about 6 kilometers deep from rim to floor, only slightly less deep than the nearby younger and much better preserved Orientale Basin (8 kilometers deep). The South Pole-Aitken Basin, the oldest discernible impact feature on the moon, is revealed as a basin 2500 kilometers in diameter with an average depth of more than 13 kilometers, rim crest to floor. This feature is the largest, deepest impact crater yet discovered in the solar system. Several additional depressions seen in the data may represent previously unmapped ancient impact basins.

  10. Ancient Multiring Basins on the Moon Revealed by Clementine Laser Altimetry

    NASA Astrophysics Data System (ADS)

    Spudis, Paul D.; Reisse, Robert A.; Gillis, Jeffrey J.

    1994-12-01

    Analysis of laser altimetry data from Clementine has confirmed and extended our knowledge of nearly obliterated multiring basins on the moon. These basins were formed during the early bombardment phase of lunar history, have been filled to varying degrees by mare lavas and regional ejecta blankets, and have been degraded by the superposition of large impact craters. The Mendel-Rydberg Basin, a degraded three-ring feature over 600 kilometers in diameter on the lunar western limb, is about 6 kilometers deep from rim to floor, only slightly less deep than the nearby younger and much better preserved Orientale Basin (8 kilometers deep). The South Pole-Aitken Basin, the oldest discernible impact feature on the moon, is revealed as a basin 2500 kilometers in diameter with an average depth of more than 13 kilometers, rim crest to floor. This feature is the largest, deepest impact crater yet discovered in the solar system. Several additional depressions seen in the data may represent previously unmapped ancient impact basins.

  11. Photoreceptor Specificity in the Light-Induced and COP1-Mediated Rapid Degradation of the Repressor of Photomorphogenesis SPA2 in Arabidopsis

    PubMed Central

    Chen, Song; Lory, Niels; Stauber, Johannes; Hoecker, Ute

    2015-01-01

    The Arabidopsis COP1/SPA E3 ubiquitin ligase is a key negative regulator that represses light signaling in darkness by targeting transcription factors involved in the light response for degradation. The COP1/SPA complex consists of COP1 and members of the four-member SPA protein family (SPA1-SPA4). Genetic analysis indicated that COP1/SPA2 function is particularly strongly repressed by light when compared to complexes carrying the other three SPAs, thereby promoting a light response after exposure of plants to extremely low light. Here, we show that the SPA2 protein is degraded within 5–15 min after exposure of dark-grown seedlings to a pulse of light. Phytochrome photoreceptors are required for the rapid degradation of SPA2 in red, far-red and also in blue light, whereas cryptochromes are not involved in the rapid, blue light-induced reduction in SPA2 protein levels. These results uncover a photoreceptor-specific mechanism of light-induced inhibition of COP1/SPA2 function. Phytochrome A (phyA) is required for the severe blue light responsiveness of spa triple mutants expressing only SPA2, thus confirming the important role of phyA in downregulating SPA2 function in blue light. In blue light, SPA2 forms a complex with cryptochrome 1 (cry1), but not with cryptochrome 2 (cry2) in vivo, indicating that the lack of a rapid blue light response of the SPA2 protein is only in part caused by a failure to interact with cryptochromes. Since SPA1 interacts with both cry1 and cry2, these results provide first molecular evidence that the light-regulation of different SPA proteins diverged during evolution. SPA2 degradation in the light requires COP1 and the COP1-interacting coiled-coil domain of SPA2, supporting that SPA2 is ubiquitinated by COP1. We propose that light perceived by phytochromes causes a switch in the ubiquitination activity of COP1/SPA2 from ubiquitinating downstream substrates to ubiquitinating SPA2, which subsequently causes a repression of COP1/SPA2

  12. Dating Melt Rock 63545 By Rb-Sr and Sm-Nd: Age of Imbrium; Spa Dress Rehearsal

    NASA Technical Reports Server (NTRS)

    Nyquist, L. E.; Shih, C. Y.; Reese, Y. D.

    2011-01-01

    Apollo 16 sample 63545 was initially described as one of a group of 19 generally rounded, fine-grained, crystalline rocks that were collected as rake samples [1]. This 16 g "rocklet" was collected at Station 13 on the ejecta blanket of North Ray Crater at the foot of Smoky Mountain [2]. Originally classified as a Very High Alumina (VHA) basalt on geochemical grounds [3], it was later argued to be an impact melt rock [4]. Here we report a Rb-Sr and Sm-Nd isotopic study that shows that some portions of the rock failed to reach isotopic equilibrium on last melting in agreement with the impact melt rock interpretation. Nevertheless, by omitting mineral fractions that are discordant with the majority of the data, we arrive at the time of last melting as 3.88 plus or minus 0.05 Ga ago. This age is in agreement with the Ar-39/Ar-40 plateau age of 3839 plus or minus 23 Ma [5], if the latter is adjusted for the 1.4-1.8% revision in the age of the hornblende monitor [6]. This investigation was undertaken in part as proof-of-concept for SPA-basin sample return.

  13. Application of WetSpa model for assessing land use impacts on floods in the Margecany-Hornad watershed, Slovakia.

    PubMed

    Bahremand, A; De Smedt, F; Corluy, J; Liu, Y B; Poórová, J; Velcická, L; Kunikova, E

    2006-01-01

    The spatially distributed hydrologic model WetSpa combines elevation, soil and land use data within GIS, to predict flood hydrographs and spatial distribution of hydrologic characteristics in a watershed. The model is applied to the Margecany-Hornad river basin (1131 km2) in Slovakia. Daily hydrometeorological data from 1991-2000, including precipitation data from nine stations, temperature data from four stations and evaporation data measured at one station are used as input to the model. Three base maps, i.e. DEM, land use and soil type are prepared in GIS form, using 100 x 100 m cell size. Results of the simulations show good agreement between calculated and measured hydrographs. The model predicts the daily/hourly hydrographs with 75-80% accuracy according to the Nash-Sutcliff criteria. For assessing the impact of land use changes on floods, the calibrated model is applied for a reforestation scenario, which considers a 50% increase of forest areas. The model results show that the reforestation scenario decreases the peak discharge by 12%. Investigation of peak discharges from the whole simulation period, shows that the scenario results are reduced by 18% on average, while for small discharges the reduction is even about 34%. The time to peak of the simulated hydrograph of the reforestation scenario is 20 hours longer than for the present land use.

  14. AGU section-wide electronic connections: A case history from SPA

    NASA Astrophysics Data System (ADS)

    Le, Guan; Russell, C. T.; Luhmann, J. G.

    In the spring of 1994 when the Internet was rapidly expanding and the World Wide Web was still developing,several of us serving in the SPA Section Executive Committee decided to launch an electronic newsletter and section Web site.The purpose of each was multifold. The newsletter would provide a way to rapidly disseminate items of recent interest, allowing widespread, immediate community response when warranted and a venue for advertising events of specific concern to SPA members and others with space physics connections. It would serve as an effective bulletin board for the exchange of useful information; for example, the launch of a spacecraft, the release of a special data set, or notes on honors bestowed upon SPA members.

  15. Has time come for a re-assessment of spa therapy? The NAIADE survey in Italy

    NASA Astrophysics Data System (ADS)

    Coccheri, S.; Gasbarrini, G.; Valenti, M.; Nappi, G.; di Orio, F.

    2008-01-01

    Goal of this study was to investigate whether appropriately applied spa therapy in several indications could be associated with a subsequent fall in the need for costly health services and missed working days due to sick-leave. The Naiade project was a multicenter observational, longitudinal, questionnaire-based study comparing an “entry” inquiry addressed to patients before an entry thermal cycle, and a “return” inquiry after 1 year. Routine statistical methods were used for comparisons. The study was carried out in 297 of the 340 certified Italian spa centers. Inquiries were managed by the spa doctor(s), with the collaboration of family doctors, and when necessary, hospitals, other health services, labour offices and employers. After exclusion of regular customers and of patients with acute disease phases or severe health conditions, 39,943 patients divided into eight diseases subgroups (rheumatic, respiratory, dermatologic, gynaecologic, otorhynologic, urinary, vascular and gastroenteric) underwent entry inquiry and appropriate spa treatment. Patients who returned for treatment after 1 year (“index year”) were 23,680 (59.2%) and received return inquiry. Outcomes considered were: frequency and duration of hospitalisation periods; missed working days; regular use of disease-specific drugs; and resort to “non-spa” rehabilitation therapies. The data collected at return inquiry were compared with those of entry inquiry. All the considered outcomes appeared to be significantly reduced in the index year in seven of the eight disease subgroups in comparison with the previous year. In conclusion, disease-appropriate spa treatments were followed by a reduction in the need of subsequent health interventions in most disease subgroups. The health promoting value of spa treatments should therefore undergo more rigorous assessment with randomised controlled studies.

  16. Spa therapy: can be a valid option for treating knee osteoarthritis?

    NASA Astrophysics Data System (ADS)

    Tenti, Sara; Cheleschi, Sara; Galeazzi, Mauro; Fioravanti, Antonella

    2015-08-01

    Osteoarthritis (OA) continues to be one of the leading causes of `years lived with disability' worldwide. Symptomatic knee OA is highly prevalent among people aged 50 years and over and is destined to become an ever more important healthcare problem. Current management of knee OA includes non-pharmacological and pharmacological treatments. Spa therapy is one of the most commonly used non-pharmacological approaches for OA in many European countries, as well as in Japan and Israel. Despite its long history and popularity, spa treatment is still the subject of debate and its role in modern medicine continues to be unclear. The objective of this review is to summarize the currently available information on clinical effects and mechanisms of action of spa therapy in knee OA. Various randomized controlled clinical trials (RCTs) were conducted to assess the efficacy and tolerability of balneotherapy and mud-pack therapy in patients with knee OA. Data from these clinical trials support a beneficial effect of spa therapy on pain, function and quality of life in knee OA that lasts over time, until 6-9 months after the treatment. The mechanisms by which immersion in mineral or thermal water or the application of mud alleviate suffering in OA are not fully understood. The net benefit is probably the result of a combination of factors, among which the mechanical, thermal and chemical effects are most prominent. In conclusion, spa therapy seems to have a role in the treatment of knee OA. Additional RCTs and further studies of mechanisms of action with high methodological quality are necessary to prove the effects of spa therapy.

  17. Spa therapy: can be a valid option for treating knee osteoarthritis?

    PubMed

    Tenti, Sara; Cheleschi, Sara; Galeazzi, Mauro; Fioravanti, Antonella

    2015-08-01

    Osteoarthritis (OA) continues to be one of the leading causes of 'years lived with disability' worldwide. Symptomatic knee OA is highly prevalent among people aged 50 years and over and is destined to become an ever more important healthcare problem. Current management of knee OA includes non-pharmacological and pharmacological treatments. Spa therapy is one of the most commonly used non-pharmacological approaches for OA in many European countries, as well as in Japan and Israel. Despite its long history and popularity, spa treatment is still the subject of debate and its role in modern medicine continues to be unclear. The objective of this review is to summarize the currently available information on clinical effects and mechanisms of action of spa therapy in knee OA. Various randomized controlled clinical trials (RCTs) were conducted to assess the efficacy and tolerability of balneotherapy and mud-pack therapy in patients with knee OA. Data from these clinical trials support a beneficial effect of spa therapy on pain, function and quality of life in knee OA that lasts over time, until 6-9 months after the treatment. The mechanisms by which immersion in mineral or thermal water or the application of mud alleviate suffering in OA are not fully understood. The net benefit is probably the result of a combination of factors, among which the mechanical, thermal and chemical effects are most prominent. In conclusion, spa therapy seems to have a role in the treatment of knee OA. Additional RCTs and further studies of mechanisms of action with high methodological quality are necessary to prove the effects of spa therapy.

  18. 78 FR 26371 - Notice of Hearing: Reconsideration of Disapproval of Kentucky State Plan Amendments (SPA) 10-007

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-06

    ... Disapproval of Kentucky State Plan Amendments (SPA) 10-007 AGENCY: Centers for Medicare & Medicaid Services..., South West, Atlanta, Georgia 30303-8909, to reconsider CMS' decision to disapprove Kentucky SPA 10-007...: This notice announces an administrative hearing to reconsider CMS's decision to disapprove Kentucky...

  19. 76 FR 34711 - Notice of Hearing; Reconsideration of Disapproval of Colorado State Plan Amendments (SPA) 10-034

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-14

    ... Disapproval of Colorado State Plan Amendments (SPA) 10-034 AGENCY: Centers for Medicare & Medicaid Services..., Suite 700, Denver, Colorado 80202-4367 to reconsider CMS' decision to disapprove Colorado SPA 10-034...: This notice announces an administrative hearing to reconsider CMS' decision to disapprove Colorado...

  20. 78 FR 40443 - Submission for OMB Review; Comment Request-Virginia Graeme Baker Pool and Spa Safety Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-05

    ... of April 19, 2013 (76 FR 23546), the Consumer Product Safety Commission (CPSC or Commission... COMMISSION Submission for OMB Review; Comment Request--Virginia Graeme Baker Pool and Spa Safety Act... verify whether pools and spas are in compliance with the Virginia Graeme Baker Pool and Spa Safety...

  1. [Spa facilities and legionellosis: legislative structure and state of the art in Apulia (Italy)].

    PubMed

    Montagna, Maria Teresa; Cuna, Teresa; De Giglio, Osvalda; Bollino, Annapaola; Iatta, Roberta; Napoli, Christian

    2012-01-01

    A number of factors, for example water temperature, can encourage the growth of microorganisms such as Legionella spp in spa facilities. Individuals who attend this type of facility are often subjects at risk for infection who are undergoing inhalation therapy and hot tub treatments. A very accurate management of these facilities is therefore required to avoid infection by Legionella spp. The purpose of this study was to verify the current Italian national and Apulia regional legislation regarding the control of contamination by Legionella spp. in spa facilities.

  2. Radon concentrations in spa water taken from hot and cold springs in Taiwan.

    PubMed

    Weng, P S; Lin, C L

    1995-05-01

    Spa water samples taken from hot and cold springs throughout Taiwan were analyzed for waterborne radon concentrations using electret ion chambers. The highest radon concentration was detected at Yangmingshan National Park, where it is closed to the action level of 11.0 kBq m-3. Next comes a sea-water hot spring at Green Isle on the east coast of Taiwan. The spa water used by the nearby inhabitants may increase the indoor radon concentration by a factor of two in extreme cases.

  3. May spa therapy be a valid opportunity to treat hand osteoarthritis? A review of clinical trials and mechanisms of action

    NASA Astrophysics Data System (ADS)

    Fortunati, Nicola Angelo; Fioravanti, Antonella; Seri, Gina; Cinelli, Simone; Tenti, Sara

    2016-01-01

    Osteoarthritis (OA) is the most common form of arthritis and its current treatment includes non-pharmacological and pharmacological modalities. Spa therapy represents a popular treatment for many rheumatic diseases. The aim of this review was to summarize the currently available information on clinical effects and mechanisms of action of spa therapy in OA of the hand. We conducted a search of the literature to extract articles describing randomized clinical trials (RCTs) in hand OA published in the period 1952-2015. We identified three assessable articles reporting RCTs on spa therapy in hand OA. Data from these clinical trials support a beneficial effect of spa therapy on pain, function and quality of life in hand OA. Spa therapy seems to have a role in the treatment of hand OA. However, additional RCTs are necessary to clarify the mechanisms of action and the effects of the application of thermal treatments.

  4. May spa therapy be a valid opportunity to treat hand osteoarthritis? A review of clinical trials and mechanisms of action.

    PubMed

    Fortunati, Nicola Angelo; Fioravanti, Antonella; Seri, Gina; Cinelli, Simone; Tenti, Sara

    2016-01-01

    Osteoarthritis (OA) is the most common form of arthritis and its current treatment includes non-pharmacological and pharmacological modalities. Spa therapy represents a popular treatment for many rheumatic diseases. The aim of this review was to summarize the currently available information on clinical effects and mechanisms of action of spa therapy in OA of the hand. We conducted a search of the literature to extract articles describing randomized clinical trials (RCTs) in hand OA published in the period 1952-2015. We identified three assessable articles reporting RCTs on spa therapy in hand OA. Data from these clinical trials support a beneficial effect of spa therapy on pain, function and quality of life in hand OA. Spa therapy seems to have a role in the treatment of hand OA. However, additional RCTs are necessary to clarify the mechanisms of action and the effects of the application of thermal treatments.

  5. Role of surfactant protein-A (SP-A) in lung injury in response to acute ozone exposure of SP-A deficient mice

    SciTech Connect

    Haque, Rizwanul; Umstead, Todd M.; Ponnuru, Padmavathi; Guo Xiaoxuan; Hawgood, Samuel; Phelps, David S.; Floros, Joanna . E-mail: jfloros@psu.edu

    2007-04-01

    Millions are exposed to ozone levels above recommended limits, impairing lung function, causing epithelial damage and inflammation, and predisposing some individuals to pneumonia, asthma, and other lung conditions. Surfactant protein-A (SP-A) plays a role in host defense, the regulation of inflammation, and repair of tissue damage. We tested the hypothesis that the lungs of SP-A(-/-) (KO) mice are more susceptible to ozone-induced damage. We compared the effects of ozone on KO and wild type (WT) mice on the C57BL/6 genetic background by exposing them to 2 parts/million of ozone for 3 or 6 h and sacrificing them 0, 4, and 24 h later. Lungs were subject to bronchoalveolar lavage (BAL) or used to measure endpoints of oxidative stress and inflammation. Despite more total protein in BAL of KO mice after a 3 h ozone exposure, WT mice had increased oxidation of protein and had oxidized SP-A dimers. In KO mice there was epithelial damage as assessed by increased LDH activity and there was increased phospholipid content. In WT mice there were more BAL PMNs and elevated macrophage inflammatory protein (MIP)-2 and monocyte chemoattractant protein (MCP)-1. Changes in MIP-2 and MCP-1 were observed in both KO and WT, however mRNA levels differed. In KO mice MIP-2 mRNA levels changed little with ozone, but in WT levels they were significantly increased. In summary, several aspects of the inflammatory response differ between WT and KO mice. These in vivo findings appear to implicate SP-A in regulating inflammation and limiting epithelial damage in response to ozone exposure.

  6. Knockdown of Drosha in human alveolar type II cells alters expression of SP-A in culture: a pilot study

    PubMed Central

    Silveyra, Patricia; Chroneos, Zissis C; DiAngelo, Susan L; Thomas, Neal J; Noutsios, Georgios T; Tsotakos, Nikolaos; Howrlylak, Judie A; Umstead, Todd M; Floros, Joanna

    2014-01-01

    Human surfactant protein A (SP-A) plays an important role in surfactant metabolism and lung innate immunity. SP-A is synthesized and secreted by alveolar type II cells (ATII), one of the two cell types of the distal lung epithelium (ATII and ATI). We have shown that miRNA interactions with sequence polymorphisms on the SP-A mRNA 3′UTRs mediate differential expression of SP-A1 and SP-A2 gene variants in vitro. In the present study, we describe a physiologically relevant model to study miRNA regulation of SP-A in human ATII. For these studies, we purified and cultured human ATII on an air-liquid interface matrix (A/L) or plastic wells without matrix (P). Gene expression analyses confirmed that cells cultured in A/L maintained the ATII phenotype for over 5 days, whereas P-cultured cells differentiated to ATI. When we transfected ATII with siRNAs to inhibit the expression of Drosha, a critical effector of miRNA maturation, the levels of SP-A mRNA and protein increased in a time dependent manner. We next characterized cultured ATII and ATI by studying expression of 1,066 human miRNAs using miRNA PCR arrays. We detected expression of >300 miRNAs with 24 miRNAs differentially expressed in ATII vs. ATI, 12 of which predicted to bind SP-A 3′UTRs, indicating that these may be implicated in SP-A downregulation in ATI. Thus, miRNAs not only affect SPA expression, but also may contribute to the maintenance of the ATII cell phenotype and/or the trans-differentiation of ATII to ATI cells, and may represent new molecular markers that distinguish ATII and ATI. PMID:25058539

  7. Pilot study exploring lung allograft surfactant protein A (SP-A) expression in association with lung transplant outcome.

    PubMed

    D'Ovidio, F; Kaneda, H; Chaparro, C; Mura, M; Lederer, D; Di Angelo, S; Takahashi, H; Gutierrez, C; Hutcheon, M; Singer, L G; Waddell, T K; Floros, J; Liu, M; Keshavjee, S

    2013-10-01

    Primary graft failure and chronic lung allograft dysfunction (CLAD) limit lung transplant long-term outcomes. Various lung diseases have been correlated with surfactant protein (SP) expression and polymorphisms. We sought to investigate the role of SP expression in lung allografts prior to implantation, in relation to posttransplant outcomes. The expression of SP-(A, B, C, D) mRNA was assayed in 42 allografts. Posttransplant assessments include pulmonary function tests, bronchoscopy, broncho-alveolar lavage fluid (BALF) and biopsies to determine allograft rejection. BALF was assayed for SP-A, SP-D in addition to cytokines IL-8, IL-12 and IL-2. The diagnosis of CLAD was evaluated 6 months after transplantation. Lung allografts with low SP-A mRNA expression prior to implantation reduced survival (Log-rank p < 0.0001). No association was noted for the other SPs. Allografts with low SP-A mRNA had greater IL-2 (p = 0.03) and IL-12 (p < 0.0001) in the BALF and a greater incidence of rejection episodes (p = 0.003). Levels of SP-A mRNA expression were associated with the SP-A2 polymorphisms (p = 0.015). Specifically, genotype 1A1A(0) was associated with lower SP-A mRNA expression (p < 0.05). Lung allografts with low levels of SP-A mRNA expression are associated with reduced survival. Lung allograft SP-A mRNA expression appears to be associated with SP-A gene polymorphisms.

  8. 77 FR 35888 - Airworthiness Directives; PIAGGIO AERO INDUSTRIES S.p.A Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-15

    ... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... PIAGGIO AERO INDUSTRIES S.p.A Model P-180 airplanes, serial numbers (S/Ns) 1002 and 1004 through 1223... flap external screwjacks installed during production. (g) Credit for Actions Accomplished in...

  9. [Measurement of soil organic matter and available K based on SPA-LS-SVM].

    PubMed

    Zhang, Hai-Liang; Liu, Xue-Mei; He, Yong

    2014-05-01

    Visible and short wave infrared spectroscopy (Vis/SW-NIRS) was investigated in the present study for measurement of soil organic matter (OM) and available potassium (K). Four types of pretreatments including smoothing, SNV, MSC and SG smoothing+first derivative were adopted to eliminate the system noises and external disturbances. Then partial least squares regression (PLSR) and least squares-support vector machine (LS-SVM) models were implemented for calibration models. The LS-SVM model was built by using characteristic wavelength based on successive projections algorithm (SPA). Simultaneously, the performance of LSSVM models was compared with PLSR models. The results indicated that LS-SVM models using characteristic wavelength as inputs based on SPA outperformed PLSR models. The optimal SPA-LS-SVM models were achieved, and the correlation coefficient (r), and RMSEP were 0. 860 2 and 2. 98 for OM and 0. 730 5 and 15. 78 for K, respectively. The results indicated that visible and short wave near infrared spectroscopy (Vis/SW-NIRS) (325 approximately 1 075 nm) combined with LS-SVM based on SPA could be utilized as a precision method for the determination of soil properties.

  10. 77 FR 30234 - Airworthiness Directives; Agusta S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-22

    ....A. Helicopters AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: We propose to adopt a new airworthiness directive (AD) for Agusta S.p.A. (Agusta... metallic spacers on some A109 model helicopters. This proposed AD would require a one-time inspection...

  11. Impact of Different Spa Waters on Inflammation Parameters in Human Keratinocyte HaCaT Cells

    PubMed Central

    Zöller, Nadja; Valesky, Eva; Hofmann, Matthias; Bereiter-Hahn, Jürgen; Bernd, August; Kaufmann, Roland; Meissner, Markus

    2015-01-01

    Background The treatment of different skin conditions with spa waters is a long tradition dating back to at least late Hellenism. Interestingly, independent scientific examinations studying the effect of spa waters are scarce. Objective In the present in vitro study, we compared the effect of culture media supplemented with (a) thermal spa waters (La Roche-Posay, Avène) and (b) two natural mineral drinking waters (Heppinger, Adelholzener) on physiological parameters in HaCaT keratinocytes. Methods The different medium preparations were investigated with regard to cell proliferation and cell damage. Moreover, the impact on inflammation parameters with and without ultraviolet B (UVB) irradiation was examined. Results Two popular thermal spring waters were found to suppress cell proliferation and cell damage. Moreover, these waters reversed the induction of interleukin-6, as measured using enzyme-linked immunosorbent assay and promoter transactivation, and the formation of reactive oxygen species after UVB stimulation. Of note, the two natural mineral waters, which are distributed as drinking waters, had some effect on the above-mentioned parameters but to a lesser extent. Conclusion In summary, our results show that spa waters, and particularly those derived from thermal springs, reduce parameters associated with inflammation. It seems likely that trace elements such as selenium and zinc are critical for the observed effects. PMID:26719640

  12. 78 FR 24367 - Airworthiness Directives; AgustaWestland S.p.A. Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-25

    ...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); 3. Will not affect...Westland S.p.A. Helicopters AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: We propose to adopt a new airworthiness directive (AD) for AgustaWestland...

  13. 75 FR 5690 - Airworthiness Directives; PIAGGIO AERO INDUSTRIES S.p.A. Model P-180 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-04

    ... specified products. That NPRM was published in the Federal Register on November 19, 2009 (74 FR 59941). That...'' under DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and (3) Will not have a... INDUSTRIES S.p.A. Model P- 180 Airplanes AGENCY: Federal Aviation Administration (FAA), Department...

  14. The privatization of spa companies in Poland - An evaluation of policy assumptions and implementation.

    PubMed

    Szromek, Adam R; Romaniuk, Piotr; Hadzik, Andrzej

    2016-04-01

    The aim of this article is to present the course of privatization of spa companies in Poland during the period 2001-2011. We discuss assumptions of the privatization process, as well as actual implementation, having identified the process as chaotic and inconsistent with prior legal provisions. We found that in its applied form the process resulted in limitation of the therapeutic potential of spas, and reduction of the State's ability to implement health policy in a legally determined form. We also found that privatization potentially improved spa infrastructure standards and increases the tourist potential of spa resorts. We recommend that clear eligibility criteria are applied to institutions in the privatization process, as well as the provision of legal guarantees for access to spa services financed from public resources. Such guarantees should be made a public obligation, to ensure the availability of services for insured persons, and there should be an obligation to maintain a specific part of a given institution's potential for the needs of patients funded by public health insurance.

  15. Getting Down to Business: Health Spa, Module 16. Teacher Guide. Entrepreneurship Training Components.

    ERIC Educational Resources Information Center

    Wolman, Jean

    This is the sixteenth in a set of 36 teacher guides to the Entrepreneurial Training Modules and accompanies CE 031 060. Its purpose is to give students some idea of what it is like to own and operate a health spa. Following an overview are general notes on use of the module. Suggested steps for module use contain suggestions on introducing the…

  16. 76 FR 62605 - Virginia Graeme Baker Pool and Spa Safety Act; Interpretation of Unblockable Drain

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... (76 FR 47436), we published a final rule to incorporate into our regulations ANSI/APSP-16 2011 as the... issued a final interpretive rule in the Federal Register (75 FR 21985) interpreting ``unblockable drain... COMMISSION 16 CFR Part 1450 Virginia Graeme Baker Pool and Spa Safety Act; Interpretation of...

  17. Getting Down to Business: Health Spa, Module 16. [Student Module]. Entrepreneurship Training Components.

    ERIC Educational Resources Information Center

    Wolman, Jean

    This module on owning and operating a health spa is one of 36 in a series on entrepreneurship. The introduction tells the student what topics will be covered and suggests other modules to read in related occupations. Each unit includes student goals, a case study, and a discussion of the unit subject matter. Learning activities are divided into…

  18. Incorporating specificity into optimization: evaluation of SPA using CSAR 2014 and CASF 2013 benchmarks.

    PubMed

    Yan, Zhiqiang; Wang, Jin

    2016-03-01

    Scoring functions of protein-ligand interactions are widely used in computationally docking software and structure-based drug discovery. Accurate prediction of the binding energy between the protein and the ligand is the main task of the scoring function. The accuracy of a scoring function is normally evaluated by testing it on the benchmarks of protein-ligand complexes. In this work, we report the evaluation analysis of an improved version of scoring function SPecificity and Affinity (SPA). By testing on two independent benchmarks Community Structure-Activity Resource (CSAR) 2014 and Comparative Assessment of Scoring Functions (CASF) 2013, the assessment shows that SPA is relatively more accurate than other compared scoring functions in predicting the interactions between the protein and the ligand. We conclude that the inclusion of the specificity in the optimization can effectively suppress the competitive state on the funnel-like binding energy landscape, and make SPA more accurate in identifying the "native" conformation and scoring the binding decoys. The evaluation of SPA highlights the importance of binding specificity in improving the accuracy of the scoring functions.

  19. spa typing and enterotoxin gene profile of Staphylococcus aureus isolated from bovine raw milk in Korea

    PubMed Central

    Hwang, Sun Young; Park, Young Kyung

    2010-01-01

    Staphylococcus aureus is a major etiological pathogen of bovine mastitis, which triggers significant economic losses in dairy herds worldwide. In this study, S. aureus strains isolated from the milk of cows suffering from mastitis in Korea were investigated by spa typing and staphylococcal enterotoxin (SE) gene profiling. Forty-four S. aureus strains were isolated from 26 farms in five provinces. All isolates grouped into five clusters and two singletons based on 14 spa types. Cluster 1 and 2 isolates comprised 38.6% and 36.4% of total isolates, respectively, which were distributed in more than four provinces. SE and SE-like toxin genes were detected in 34 (77.3%) isolates and the most frequently detected SE gene profile was seg, sei, selm, seln, and selo genes (16 isolates, 36.3%), which was comparable to one of the genomic islands, Type I νSaβ. This is a first report of spa types and the prevalence of the recently described SE and SE-like toxin genes among S. aureus isolates from bovine raw milk in Korea. Two predominant spa groups were distributed widely and recently described SE and SE-like toxin genes were detected frequently. PMID:20458153

  20. 75 FR 65224 - Airworthiness Directives; Agusta S.p.A. Model A109E Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-22

    ... INFORMATION: This AD, Amendment 39-16456 (75 FR 61341, October 5, 2010), requires modifying the fuselage... (75 FR 61341), on page 61342, in the third column, under ``Sec. 39.13 ,'' the AD number is corrected...-38-AD; Amendment 39-16456; AD 2010-20-21] RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A....

  1. 77 FR 2493 - Special Local Regulations for Marine Events; Spa Creek and Annapolis Harbor, Annapolis, MD

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-18

    ... ``TriRock Triathlon Series'', a marine event to be held on the waters of Spa Creek and Annapolis Harbor...), U.S. Department of Transportation, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue... Houck, U.S. Coast Guard Sector Baltimore, MD; telephone (410) 576-2674, email...

  2. 76 FR 9273 - Special Local Regulations for Marine Events; Severn River, Spa Creek and Annapolis Harbor...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-17

    ... Federal Register (73 FR 3316). Public Meeting We do not now plan to hold a public meeting. But you may... swim segment of the ``TriRock Annapolis'' triathlon, a marine event to be held on the waters of Spa... Diego, California, will sponsor the ``TriRock Annapolis'' triathlon in Annapolis, Maryland. The...

  3. The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time.

    PubMed

    Ranjan, Aashish; Fiene, Gabriele; Fackendahl, Petra; Hoecker, Ute

    2011-05-01

    Plants adjust their growth and development in response to the ambient light environment. These light responses involve systemic signals that coordinate differentiation of different tissues and organs. Here, we have investigated the function of the key repressor of photomorphogenesis SPA1 in different tissues of the plant by expressing GUS-SPA1 under the control of tissue-specific promoters in a spa mutant background. We show that SPA1 expression in the phloem vasculature is sufficient to rescue the spa1 mutant phenotype in dark-grown spa mutant seedlings. Expression of SPA1 in mesophyll, epidermis or root tissues of the seedling, by contrast, has no or only slight effects. In the leaf, SPA1 expression in both the phloem and the mesophyll is required for full complementation of the defect in leaf expansion. SPA1 in phloem and mesophyll tissues affected division and expansion of cells in the epidermal layer, indicating that SPA1 induces non-cell-autonomous responses also in the leaf. Photoperiodic flowering is exclusively controlled by SPA1 expression in the phloem, which is consistent with previous results showing that the direct substrate of the COP1/SPA complex, CONSTANS, also acts in the phloem. Taken together, our results highlight the importance of phloem vascular tissue in coordinating growth and development. Because the SPA1 protein itself is incapable of moving from cell to cell, we suggest that SPA1 regulates the activity of downstream component(s) of light signaling that subsequently act in a non-cell-autonomous manner. SPA1 action in the phloem may also result in mechanical stimuli that affect cell elongation and cell division in other tissues.

  4. Disinfection of herbal spa pool using combined chlorine dioxide and sodium hypochlorite treatment.

    PubMed

    Hsu, Ching-Shan; Huang, Da-Ji

    2015-02-01

    The presence of pathogenic microorganisms in public spa pools poses a serious threat to human health. The problem is particularly acute in herbal spas, in which the herbs and microorganisms may interact and produce undesirable consequences. Accordingly, the present study investigated the effectiveness of a combined disinfectant containing chlorine dioxide and sodium hypochlorite in improving the water quality of a public herbal spa in Taiwan. Water samples were collected from the spa pool and laboratory tests were then performed to measure the variation over time of the microorganism content (total CFU and total coliforms) and residual disinfectant content given a single disinfection mode (SDM) with disinfectant concentrations of 5.2 × 10, 6.29 × 10, 7.4 × 10, and 11.4 × 10(-5) N, respectively. Utilizing the experience gained from the laboratory tests, a further series of on-site investigations was performed using three different disinfection modes, namely SDM, 3DM (once every 3 h disinfection mode), and 2DM (once every 2 h disinfection mode). The laboratory results showed that for all four disinfectant concentrations, the CFU concentration reduced for the first 6 h following SDM treatment, but then increased. Moreover, the ANOVA results showed that the sample treated with the highest disinfectant concentration (11.4 × 10(-5) N) exhibited the lowest rate of increase in the CFU concentration. In addition, the on-site test results showed that 3DM and 2DM treatments with disinfectant concentrations in excess of 9.3 × 10 and 5.5 × 10(-5) N, respectively, provided an effective reduction in the total CFU concentration. In conclusion, the experimental results presented in this study provide a useful source of reference for spa businesses seeking to improve the water quality of their spa pools.

  5. Disinfection of herbal spa pool using combined chlorine dioxide and sodium hypochlorite treatment.

    PubMed

    Hsu, Ching-Shan; Huang, Da-Ji

    2015-02-01

    The presence of pathogenic microorganisms in public spa pools poses a serious threat to human health. The problem is particularly acute in herbal spas, in which the herbs and microorganisms may interact and produce undesirable consequences. Accordingly, the present study investigated the effectiveness of a combined disinfectant containing chlorine dioxide and sodium hypochlorite in improving the water quality of a public herbal spa in Taiwan. Water samples were collected from the spa pool and laboratory tests were then performed to measure the variation over time of the microorganism content (total CFU and total coliforms) and residual disinfectant content given a single disinfection mode (SDM) with disinfectant concentrations of 5.2 × 10, 6.29 × 10, 7.4 × 10, and 11.4 × 10(-5) N, respectively. Utilizing the experience gained from the laboratory tests, a further series of on-site investigations was performed using three different disinfection modes, namely SDM, 3DM (once every 3 h disinfection mode), and 2DM (once every 2 h disinfection mode). The laboratory results showed that for all four disinfectant concentrations, the CFU concentration reduced for the first 6 h following SDM treatment, but then increased. Moreover, the ANOVA results showed that the sample treated with the highest disinfectant concentration (11.4 × 10(-5) N) exhibited the lowest rate of increase in the CFU concentration. In addition, the on-site test results showed that 3DM and 2DM treatments with disinfectant concentrations in excess of 9.3 × 10 and 5.5 × 10(-5) N, respectively, provided an effective reduction in the total CFU concentration. In conclusion, the experimental results presented in this study provide a useful source of reference for spa businesses seeking to improve the water quality of their spa pools. PMID:25632897

  6. Epithelial Adhesion Mediated by Pilin SpaC Is Required for Lactobacillus rhamnosus GG-Induced Cellular Responses

    PubMed Central

    Ardita, Courtney S.; Mercante, Jeffrey W.; Kwon, Young Man; Luo, Liping; Crawford, Madelyn E.; Powell, Domonica N.; Jones, Rheinallt M.

    2014-01-01

    Lactobacillus rhamnosus GG is a widely used probiotic, and the strain's salutary effects on the intestine have been extensively documented. We previously reported that strain GG can modulate inflammatory signaling, as well as epithelial migration and proliferation, by activating NADPH oxidase 1-catalyzed generation of reactive oxygen species (ROS). However, how strain GG induces these responses is unknown. Here, we report that strain GG's probiotic benefits are dependent on the bacterial-epithelial interaction mediated by the SpaC pilin subunit. By comparing strain GG to an isogenic mutant that lacks SpaC (strain GGΩspaC), we establish that SpaC is necessary for strain GG to adhere to gut mucosa, that SpaC contributes to strain GG-induced epithelial generation of ROS, and that SpaC plays a role in strain GG's capacity to stimulate extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) signaling in enterocytes. In addition, we show that SpaC is required for strain GG-mediated stimulation of cell proliferation and protection against radiologically inflicted intestinal injury. The identification of a critical surface protein required for strain GG to mediate its probiotic influence advances our understanding of the molecular basis for the symbiotic relationship between some commensal bacteria of the gut lumen and enterocytes. Further insights into this relationship are critical for the development of novel approaches to treat intestinal diseases. PMID:24928883

  7. Epithelial adhesion mediated by pilin SpaC is required for Lactobacillus rhamnosus GG-induced cellular responses.

    PubMed

    Ardita, Courtney S; Mercante, Jeffrey W; Kwon, Young Man; Luo, Liping; Crawford, Madelyn E; Powell, Domonica N; Jones, Rheinallt M; Neish, Andrew S

    2014-08-01

    Lactobacillus rhamnosus GG is a widely used probiotic, and the strain's salutary effects on the intestine have been extensively documented. We previously reported that strain GG can modulate inflammatory signaling, as well as epithelial migration and proliferation, by activating NADPH oxidase 1-catalyzed generation of reactive oxygen species (ROS). However, how strain GG induces these responses is unknown. Here, we report that strain GG's probiotic benefits are dependent on the bacterial-epithelial interaction mediated by the SpaC pilin subunit. By comparing strain GG to an isogenic mutant that lacks SpaC (strain GGΩspaC), we establish that SpaC is necessary for strain GG to adhere to gut mucosa, that SpaC contributes to strain GG-induced epithelial generation of ROS, and that SpaC plays a role in strain GG's capacity to stimulate extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) signaling in enterocytes. In addition, we show that SpaC is required for strain GG-mediated stimulation of cell proliferation and protection against radiologically inflicted intestinal injury. The identification of a critical surface protein required for strain GG to mediate its probiotic influence advances our understanding of the molecular basis for the symbiotic relationship between some commensal bacteria of the gut lumen and enterocytes. Further insights into this relationship are critical for the development of novel approaches to treat intestinal diseases.

  8. Immobilization of Bioactive Protein A from Staphylococcus aureus (SpA) on the Surface of Bacillus subtilis Spores.

    PubMed

    Ghaedmohammadi, Samira; Rigi, Garshasb; Zadmard, Reza; Ricca, Ezio; Ahmadian, Gholamreza

    2015-08-01

    Protein A from Staphylococcus aureus (SpA) is a 40-60 kDa cell-wall component, composed of five homologous immunoglobulin (Ig)-binding domains folded into a three-helix bundle. Each of these five domains is able to bind Igs from many different mammalian species. Recombinant SpA is widely used as a component of diagnostic kits for the detection and purification of IgGs from serum or other biological fluids. In this study, purified SpA was adsorbed and covalently linked to Bacillus subtilis spores. Spores are extremely stable cell forms and are considered as an attractive platform to display heterologous proteins. A sample containing about 36 μg of SpA was covalently immobilized on the surface of 4 × 10(10) spores. Spore-bound SpA retained its IgG-binding activity, even after seven consecutive binding and washing steps, suggesting that it can be recycled and utilized several times. FACS analysis revealed that spores with covalently attached SpA had significantly improved fluorescence intensities when compared to those of spores with adsorbed SpA, suggesting that the covalent approach is more efficient than sole adsorption regarding protein attachment to the spore surface.

  9. 76 FR 63161 - Airworthiness Directives; Aviointeriors S.p.A. Passenger Seat 12M Series, Installed on But Not...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... Aviointeriors S.p.A., Engineering Product Support Division, Via Appia KM 66,400--04013 Tor Tre Ponti,...

  10. [The topical problems of pediatric balneotherapy and the spa and health resort-based treatment of the children].

    PubMed

    Razumov, A N; Khan, M A

    2016-01-01

    This article is devoted to the topical problems of pediatric balneotherapy with special reference to the organization of the spa and health resort-based treatment of the children in the Russian Federation. The main issues discussed by the authors include the current state of health resort care for the children, the problem of statutory regulation of the activities of the children's spa and health resort facilities, the approaches to increasing the availability of the spa and health resort-based treatment for the children at the enhanced risk of the development of chronic diseases, disabilities, and tuberculosis. Also considered are the problems of the development of the regulatory framework for the medical rehabilitation of the children based at the spa and health resort facilities. The principal goals to be sought in climatotherapy, physiotherapy, balneotherapy, and pelotherapy in the pediatric context are outlined along with the further prospects for the development of the main areas of pediatric balneology.

  11. Cloning, auto-induction expression, and purification of rSpaA swine erysipelas antigen.

    PubMed

    da Silva, Adilson José; da Costa Iemma, Mônica Rosas; Luperni Horta, Antônio Carlos; Sargo, Cíntia Regina; de Lima Camargo Giordano, Raquel; de Campos Giordano, Roberto; Zangirolami, Teresa Cristina; Marques Novo, Maria Teresa

    2012-10-01

    This work reports the cloning, expression, and purification of a 42-kDa fragment of the SpaA protein from Erysipelothrix rhusiopathiae, the main antigenic candidate for a subunit vaccine against swine erysipelas. The use of an auto-induction protocol to improve heterologous protein expression in recombinant Escherichia coli cultures was also investigated. The cellular growth pattern and metabolite formation were evaluated under different induction conditions. The His-tagged protein was over-expressed as inclusion bodies, and was purified by a single chromatography step under denaturing conditions. Auto-induction conditions were shown to be an excellent process strategy, leading to a high level of rSpaA expression (about 25 % of total cellular protein content) in a short period of time.

  12. 222Rn in Greek spa waters: correlation with rainfall and seismic activities.

    PubMed

    Danali-Cotsaki, S; Margomenou-Leonidopoulou, G

    1993-06-01

    In our previous research, the natural radioisotopes (including 222Rn) present in the waters of the Greek spas Ikaria, Kamena Vourla, and Loutraki, were determined by gamma-spectroscopy, laboratory method. Large variations of 222Rn were found in different samples from the same spa. In order to explain these variations, the effect of rainfall before the sampling date was examined. It was concluded that rainfall decreased (or drought increased) the 222Rn concentrations in most cases. The explanation for the exceptions may be the effect of earthquakes. Characteristics considered include time, depth, magnitude, and distance of the epicenters from the spa. From the results, it can be concluded that the earthquakes increased 222Rn concentrations.

  13. Evaluation of rep-PCR/DiversiLab versus PFGE and spa typing in genotyping methicillin-resistant Staphylococcus aureus (MRSA).

    PubMed

    Aguadero, V; González Velasco, C; Vindel, A; Gonzalez Velasco, M; Moreno, J J

    2015-01-01

    Pulsed-field gel electrophoresis (PFGE) is the 'gold standard' for genotyping of methicillin-resistant Staphylococcus aureus (MRSA); however, the DiversiLab (DL) system, based on rep-PCR, is faster, simpler and could be better adapted to daily routine hospital work. We genotyped 100 MRSA isolates using PFGE, DL, and spa typing, and evaluated the discriminatory power of each technique and the correlation between them by Simpson's index(SI) and adjusted Rand coefficient (ARI), respectively. The isolates were from clinical samples from eight hospitals in Extremadura (Spain) during 2010. DL separated the 100 MRSA into 18 patterns, with 69% of the isolates grouped into four predominant patterns. spa typing reported 17 spa types, classifying 69% of MRSA into two major types (t067 and t002). PFGE revealed the existence of 27 patterns, gathering 54% of MRSA into three pulse types (E8a, E7a and E7b). SI values were 0.819, 0.726, 0.887 and 0.460 for DL, spa typing, PFGE and CC-BURP, respectively. ARI values of DL over PFGE, spa typing and CC-BURP were 0.151, 0.321 and 0.071, respectively. DL has less discriminatory power than PFGE but more than spa typing. The concordance of DL with PFGE is low, primarily because DL does not discriminate between the three predominant MRSA pulse types in our environment.

  14. Lung surfactant protein A (SP-A) interactions with model lung surfactant lipids and an SP-B fragment.

    PubMed

    Sarker, Muzaddid; Jackman, Donna; Booth, Valerie

    2011-06-01

    Surfactant protein A (SP-A) is the most abundant protein component of lung surfactant, a complex mixture of proteins and lipids. SP-A performs host defense activities and modulates the biophysical properties of surfactant in concerted action with surfactant protein B (SP-B). Current models of lung surfactant mechanism generally assume SP-A functions in its octadecameric form. However, one of the findings of this study is that when SP-A is bound to detergent and lipid micelles that mimic lung surfactant phospholipids, it exists predominantly as smaller oligomers, in sharp contrast to the much larger forms observed when alone in water. These investigations were carried out in sodium dodecyl sulfate (SDS), dodecylphosphocholine (DPC), lysomyristoylphosphatidylcholine (LMPC), lysomyristoylphosphatidylglycerol (LMPG), and mixed LMPC + LMPG micelles, using solution and diffusion nuclear magnetic resonance (NMR) spectroscopy. We have also probed SP-A's interaction with Mini-B, a biologically active synthetic fragment of SP-B, in the presence of micelles. Despite variations in Mini-B's own interactions with micelles of different compositions, SP-A is found to interact with Mini-B in all micelle systems and perhaps to undergo a further structural rearrangement upon interacting with Mini-B. The degree of SP-A-Mini-B interaction appears to be dependent on the type of lipid headgroup and is likely mediated through the micelles, rather than direct binding.

  15. Evaluation of rep-PCR/DiversiLab versus PFGE and spa typing in genotyping methicillin-resistant Staphylococcus aureus (MRSA).

    PubMed

    Aguadero, V; González Velasco, C; Vindel, A; Gonzalez Velasco, M; Moreno, J J

    2015-01-01

    Pulsed-field gel electrophoresis (PFGE) is the 'gold standard' for genotyping of methicillin-resistant Staphylococcus aureus (MRSA); however, the DiversiLab (DL) system, based on rep-PCR, is faster, simpler and could be better adapted to daily routine hospital work. We genotyped 100 MRSA isolates using PFGE, DL, and spa typing, and evaluated the discriminatory power of each technique and the correlation between them by Simpson's index(SI) and adjusted Rand coefficient (ARI), respectively. The isolates were from clinical samples from eight hospitals in Extremadura (Spain) during 2010. DL separated the 100 MRSA into 18 patterns, with 69% of the isolates grouped into four predominant patterns. spa typing reported 17 spa types, classifying 69% of MRSA into two major types (t067 and t002). PFGE revealed the existence of 27 patterns, gathering 54% of MRSA into three pulse types (E8a, E7a and E7b). SI values were 0.819, 0.726, 0.887 and 0.460 for DL, spa typing, PFGE and CC-BURP, respectively. ARI values of DL over PFGE, spa typing and CC-BURP were 0.151, 0.321 and 0.071, respectively. DL has less discriminatory power than PFGE but more than spa typing. The concordance of DL with PFGE is low, primarily because DL does not discriminate between the three predominant MRSA pulse types in our environment. PMID:26510268

  16. The effects of combined spa therapy and rehabilitation on patients with ankylosing spondylitis being treated with TNF inhibitors.

    PubMed

    Ciprian, Luca; Lo Nigro, Alessandro; Rizzo, Michela; Gava, Alessandra; Ramonda, Roberta; Punzi, Leonardo; Cozzi, Franco

    2013-01-01

    Despite advances in pharmacological therapy, physical treatment continues to be important in the management of ankylosing spondylitis (AS). The objective of the present study was to evaluate the effects and tolerability of combined spa therapy and rehabilitation in a group of AS patients being treated with TNF inhibitors. Thirty AS patients attending the Rheumatology Unit of the University of Padova being treated with TNF inhibitors for at least 3 months were randomized and assessed by an investigator independent from the spa staff: 15 were prescribed 10 sessions of spa therapy (mud packs and thermal baths) and rehabilitation (exercises in a thermal pool) and the other 15 were considered controls. The patients in both groups had been receiving anti-TNF agents for at least three months. The outcome measures utilized were BASFI, BASDAI, BASMI, VAS for back pain and HAQ. The evaluations were performed in all patients at the entry to the study, at the end of the spa treatment, and after 3 and 6 months. Most of the evaluation indices were significantly improved at the end of the spa treatment, as well as at the 3 and 6 months follow-up assessments. No significant alterations in the evaluation indices were found in the control group. Combined spa therapy and rehabilitation caused a clear, long-term clinical improvement in AS patients being treated with TNF inhibitors. Thermal treatment was found to be well tolerated and none of the patients had disease relapse.

  17. Evaluation of adding a second marker to overcome Staphylococcus aureus spa typing homoplasies.

    PubMed

    Basset, P; Nübel, U; Witte, W; Blanc, D S

    2012-04-01

    The utility of sequencing a second highly variable locus in addition to the spa gene (e.g., double-locus sequence typing [DLST]) was investigated to overcome limitations of a Staphylococcus aureus single-locus typing method. Although adding a second locus seemed to increase discriminatory power, it was not sufficient to definitively infer evolutionary relationships within a single multilocus sequence type (ST-5). PMID:22259202

  18. Molecular characterization of Staphylococcus aureus isolates from southwest of Iran using spa and SCCmec typing methods.

    PubMed

    Darban-Sarokhalil, Davood; Khoramrooz, Seyed Sajjad; Marashifard, Masoud; Malek Hosseini, Seyed Ali Asghar; Parhizgari, Najmeh; Yazdanpanah, Mahboobeh; Gharibpour, Farzaneh; Mirzaii, Mehdi; Sharifi, Bahman; Haeili, Mehri

    2016-09-01

    Staphylococcus aureus remains a major cause of nosocomial infection worldwide. Characterization of S. aureus isolates circulating in the southwest of Iran will contribute to understand and control the spread of the strains in this area. spa and SCCmec typing methods were used for genotyping of 125 S. aureus isolates obtained from two teaching hospitals in Ahvaz. Drug susceptibility testing was performed by using disk diffusion method. Frequency of the methicillin resistant S. aureus (MRSA) isolates was 39% (n = 34) and 27% (n = 10) in Emam Khomeini and Golestan hospitals, respectively. Except for Erythromycin, MRSA strains showed high rate of resistance to antimicrobial agents including penicillin (100%), norfloxacine (80%), azitromycin (80%), ciprofloxacin (80%), gentamycin (77%), cotrimoxazole (75%), cephotaxime. All isolates were sensitive to vancomycin. Out of 44 MRSA strains, 39 (88.5%) were SCCmec III, three (7%) were IVc and two (4.5%) of them were nontypeable. spa types t037 (26 isolates; 59%), and t1149 (25 isolates; 31%) were the most dominant types found in MRSA and methicillin sensitive S. aureus (MSSA) strains, respectively. We found SCCmec type III as the most prominent type indicating that most of the studied bacterial population had hospital origin. spa type t037, the most frequent genotype in this study were significantly (100%) associated with MRSA. For the first time we are reporting spa types t692, t706 and t018 from Iran and t342, t704, t2622, t5598, t11270 and t2864 from Asia. Moreover we are reporting types t6871 and t2684 for the second time in the world.

  19. Distribution of toxin genes among different spa types and phage types of animal Staphylococcus aureus.

    PubMed

    Garbacz, Katarzyna; Piechowicz, Lidia; Mroczkowska, Aneta

    2015-09-01

    We analyzed distribution of toxin genes (sea-seo, eta, etb, tst, lukS/lukF-PV) among spa types and phage types of 39 Staphylococcus aureus (S. aureus) isolates from healthy and diseased animals. All isolates turned out to be mecA negative (MSSA). Nine spa types were identified: t144 and t723 (dogs), t084 (dogs and pigs), t5447 (cat), t1491 and t008 (pigs), t002, t127 and t3478 (poultry). Seven phage types were detected, enclosed within four phage groups: I (cat), II (dogs), III (pigs) and mixed group (dogs and pigs). Three poultry spa types proved to be non-typeable by phages. Toxin genes were detected in 33 out of the 39 animal isolates. Our analysis revealed that the incidence of some toxin genes in S. aureus is host specific. Canine isolates t144 of phage group II harbored exfoliative toxin gene (eta), and porcine isolates type t1491 representing phage group III showed enterotoxin A gene (sea). The enterotoxin gene cluster (egc1) and enterotoxin gene seh were found in non-typeable isolates from chicken and in one feline isolate type t5447.

  20. [The largest outbreak of legionellosis in Japan associated with spa baths: epidemic curve and environmental investigation].

    PubMed

    Okada, Mika; Kawano, Kimiko; Kura, Fumiaki; Amemura-Maekawa, Junko; Watanabe, Haruo; Yagita, Kenji; Endo, Takuro; Suzuki, Sen

    2005-06-01

    In July 2002, a large outbreak of legionellosis occurred in a bathhouse with spa facilities in Miyazaki Prefecture. Two hundred-ninety-five patients (including suspected cases) that had pneumonia and/or symptoms of fever, cough and so forth were reported; 37% of them were hospitalized and seven people died. In environmental investigations, Legionella pneumophila serogroups (SGs) land 8, L. dumoffii, L. londiniensis, some other Legionella species and many kinds of amoeba were isolated from 55 samples of bathtub water, tank water, filters and so forth in the spa facilities. The dominant isolates from the bathtab waters belonged to L. londiniensis, L. dumoffii and L. pneumophila SG1, and their maximum concentrations were 1.5 x 10(6), 5.2 x 10(5) and 1.6 x 10(5) cfu/100 mL, respectively. L. pneumophila SG1 strains isolated from bathtub water, tank water, filters and sputa of patients showed a indistinguishable DNA fingerprint pattern by pulsed-field gel electrophoresis (PFGE), confirming that the source of infection was the spa water. Our study indicate that spas may be a significant health hazard if hygienic management fails.

  1. Crystal structure of Spa40, the specificity switch for the Shigella flexneri type III secretion system

    PubMed Central

    Deane, Janet E; Graham, Stephen C; Mitchell, Edward P; Flot, David; Johnson, Steven; Lea, Susan M

    2008-01-01

    The pathogenic bacterium Shigella flexneri uses a type III secretion system to inject virulence factors from the bacterial cytosol directly into host cells. The machinery that identifies secretion substrates and controls the export of extracellular components and effector proteins consists of several inner-membrane and cytoplasmic proteins. One of the inner membrane components, Spa40, belongs to a family of proteins proposed to regulate the switching of substrate specificity of the export apparatus. We show that Spa40 is cleaved within the strictly conserved amino acid sequence NPTH and substitution of the proposed autocatalytic residue abolishes cleavage. Here we also report the crystal structure of the cytoplasmic complex Spa40C and compare it with the recent structures of the homologues from Escherichia coli and Salmonella typhimurium. These structures reveal the tight association of the cleaved fragments and show that the conserved NPTH sequence lies on a loop which, when cleaved, swings away from the catalytic N257 residue, resulting in different surface features in this region. This structural rearrangement suggests a mechanism by which non-cleaving forms of these proteins interfere with correct substrate switching of the apparatus. PMID:18485071

  2. Ar-40-Ar-39 Age of an Impact-Melt Lithology in Lunar Meteorite Dhofar 961

    NASA Technical Reports Server (NTRS)

    Cohen, Barbara; Frasl, Barbara; Jolliff, Brad; Korotev, Randy; Zeigler, Ryan

    2016-01-01

    The Dhofar 961 lunar meteorite was found in 2003 in Oman. It is texturally paired with Dhofar 925 and Dhofar 960 (though Dhofar 961 is more mafic and richer in incompatible elements). Several lines of reasoning point to the South Pole-Aitken Basin (SPA) basin as a plausible source (Figure 2): Mafic character of the melt-breccia lithic clasts consistent the interior of SPA, rules out feldspathic highlands. Compositional differences from Apollo impact-melt groups point to a provenance that is separated and perhaps far distant from the Procellarum KREEP Terrane SPA "hot spots" where Th concentrations reach 5 ppm and it has a broad "background" of about 2 ppm, similar to lithic clasts in Dhofar 961 subsamples If true, impact-melt lithologies in this meteorite may be unaffected by the Imbrium-forming event that is pervasively found in our Apollo sample collection, and instead record the early impact history of the Moon.

  3. Wolf: What's On the Lunar Farside?

    NASA Technical Reports Server (NTRS)

    2008-01-01

    WOLF (What's On the Lunar Farside?) is a lunar sample return mission to the South Pole-Aitken (SPA) Basin, located on the farside of the moon, seeking to answer some of the remaining questions about our solar system. Through the return and analysis of SPA samples, scientists can constrain the period of inner solar system late heavy bombardment and gain momentous knowledge of the SPA basin. WOLF provides the opportunity for mankind's progression in further understanding our solar system, its history, and unknowns surrounding the lunar farside. The orbiter will provide intermittent, direct communication between the lander and ground operations via the Deep Space Network (DSN). Received images and spectrometry will aid in real-time sample selection.

  4. [Measurement of Soil Total Nitrogen Using Near Infrared Spectroscopy Combined with RCA and SPA].

    PubMed

    Fang, Xiao-rong; Zhang, Hai-liang; Huang, Ling-xia; He, Yong

    2015-05-01

    Visible near spectra tecnnology was adopted to detect soil total nitrogen content. 394 soil samples were collected from Wencheng, Zhejiang province to be used for calibration model (n=263) and independent prediction set (n=131). Raw spectra and wavelength-reduced spectra with five different pretreatment methods (SG smoothing, SNV, MSC, 1st-D and 2nd-D) were compared to determine the optimal wavelength range and pretreatment method for analysis. The results with 5 different pretreatment methods were not improved compared to that both of full spectra PLS model and wavelength reduction spectra model. Spectral variable selection is an important strategy in spectrum modeling analysis, because it tends to parsimonious data representation and can lead to multivariate models with better performance. In order to simply calibration models, the wavelength variables selected by two different variable selection methods (i. e. regression coefficient analysis (RCA) and successive projections algorithm (SPA) were proposed to be the inputs of calibration methods of PLS, MLR and LS-SVM models separately. These calibration models were also compared to select the best model to predict soil TN. In total, 9 different models were built ahd the best results indicated that PLS, MLR and LS-SVM obtained the highest precision with determination coefficient of prediction R2(pre) =0. 81, RMSEP=0. 0031 and RPD=2. 26 based on wavelength variables selected by RCA (0. 0002) and SPA as inputs of models. SPA-MLR model and other three models based on 7 sensitive variables selected by RC using 0. 0002 regression coefficient threshold value obtained the best result with R2(pre), RMSEP and RPD as 0. 81, 0. 0031 and 2. 26. This prediction accuracy is classied to be very good. For all the models, it could be concluded that RCA and SPA could be very useful ways to selected sensitive wavelengths, and the selected wavelengths were effective to estimate soil TN. It is recommended to adopt SPA variable

  5. Comparison of the DiversiLab repetitive element PCR system with spa typing and pulsed-field gel electrophoresis for clonal characterization of methicillin-resistant Staphylococcus aureus.

    PubMed

    Babouee, B; Frei, R; Schultheiss, E; Widmer, A F; Goldenberger, D

    2011-04-01

    The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has become an increasing problem worldwide in recent decades. Molecular typing methods have been developed to identify clonality of strains and monitor spread of MRSA. We compared a new commercially available DiversiLab (DL) repetitive element PCR system with spa typing, spa clonal cluster analysis, and pulsed-field gel electrophoresis (PFGE) in terms of discriminatory power and concordance. A collection of 106 well-defined MRSA strains from our hospital was analyzed, isolated between 1994 and 2006. In addition, we analyzed 6 USA300 strains collected in our institution. DL typing separated the 106 MRSA isolates in 10 distinct clusters and 8 singleton patterns. Clustering analysis into spa clonal complexes resulted in 3 clusters: spa-CC 067/548, spa-CC 008, and spa-CC 012. The discriminatory powers (Simpson's index of diversity) were 0.982, 0.950, 0.846, and 0.757 for PFGE, spa typing, DL typing, and spa clonal clustering, respectively. DL typing and spa clonal clustering showed the highest concordance, calculated by adjusted Rand's coefficients. The 6 USA300 isolates grouped homogeneously into distinct PFGE and DL clusters, and all belonged to spa type t008 and spa-CC 008. Among the three methods, DL proved to be rapid and easy to perform. DL typing qualifies for initial screening during outbreak investigation. However, compared to PFGE and spa typing, DL typing has limited discriminatory power and therefore should be complemented by more discriminative methods in isolates that share identical DL patterns.

  6. Characterisation of Shigella Spa33 and Thermotoga FliM/N reveals a new model for C‐ring assembly in T3SS

    PubMed Central

    McDowell, Melanie A.; Marcoux, Julien; McVicker, Gareth; Johnson, Steven; Fong, Yu Hang; Stevens, Rebecca; Bowman, Lesley A. H.; Degiacomi, Matteo T.; Yan, Jun; Wise, Adam; Friede, Miriam E.; Benesch, Justin L. P.; Deane, Janet E.; Tang, Christoph M.; Robinson, Carol V.

    2015-01-01

    Summary Flagellar type III secretion systems (T3SS) contain an essential cytoplasmic‐ring (C‐ring) largely composed of two proteins FliM and FliN, whereas an analogous substructure for the closely related non‐flagellar (NF) T3SS has not been observed in situ. We show that the spa33 gene encoding the putative NF‐T3SS C‐ring component in S higella flexneri is alternatively translated to produce both full‐length (Spa33‐FL) and a short variant (Spa33‐C), with both required for secretion. They associate in a 1:2 complex (Spa33‐FL/C2) that further oligomerises into elongated arrays in vitro. The structure of Spa33‐C 2 and identification of an unexpected intramolecular pseudodimer in Spa33‐FL reveal a molecular model for their higher order assembly within NF‐T3SS. Spa33‐FL and Spa33‐C are identified as functional counterparts of a FliM–FliN fusion and free FliN respectively. Furthermore, we show that T hermotoga maritima  FliM and FliN form a 1:3 complex structurally equivalent to Spa33‐FL/C2, allowing us to propose a unified model for C‐ring assembly by NF‐T3SS and flagellar‐T3SS. PMID:26538516

  7. Purification, crystallization and preliminary X-ray diffraction analysis of SpaD, a backbone-pilin subunit encoded by the fimbrial spaFED operon in Lactobacillus rhamnosus GG.

    PubMed

    Chaurasia, Priyanka; von Ossowski, Ingemar; Palva, Airi; Krishnan, Vengadesan

    2015-01-01

    SpaD is the predicted backbone-pilin subunit of the SpaFED pilus, whose loci are encoded by the fimbrial spaFED operon in Lactobacillus rhamnosus GG, a Gram-positive gut-adapted commensal strain with perceived probiotic benefits. In this study, soluble recombinant SpaD protein was overproduced in Escherichia coli and then purified by Ni2+-chelating affinity and gel-filtration chromatography. After limited proteolysis with α-chymotrypsin, good-quality crystals of SpaD were obtained which diffracted beyond 2.0 Å resolution. These crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a=50.11, b=83.27, c=149.65 Å. For phasing, sodium iodide-derivatized crystals were prepared using the halide quick-soaking method and diffraction data were collected in-house to a resolution of 2.2 Å. An interpretable electron-density map was successfully obtained using single-wavelength anomalous diffraction (SAD).

  8. Parana basin

    SciTech Connect

    Zalan, P.V.; Wolff, S.; Conceicao, J.C.J.; Vieira, I.S.; Astolfi, M.A.; Appi, V.T.; Zanotto, O.; Neto, E.V.S.; Cerqueira, J.R.

    1987-05-01

    The Parana basin is a large intracratonic basin in South America, developed entirely on continental crust and filled with sedimentary and volcanic rocks ranging in age from Silurian to Cretaceous. It occupies the southern portion of Brazil (1,100,000 km/sup 2/ or 425,000 mi/sup 2/) and the eastern half of Paraguay (100,000 km/sup 2/ or 39,000 mi/sup 2/); its extension into Argentina and Uruguay is known as the Chaco-Parana basin. Five major depositional sequences (Silurian, Devonian, Permo-Carboniferous, Triassic, Juro-Cretaceous) constitute the stratigraphic framework of the basin. The first four are predominantly siliciclastic in nature, and the fifth contains the most voluminous basaltic lava flows of the planet. Maximum thicknesses are in the order of 6000 m (19,646 ft). The sequences are separated by basin wide unconformities related in the Paleozoic to Andean orogenic events and in the Mesozoic to the continental breakup and sea floor spreading between South America and Africa. The structural framework of the Parana basin consists of a remarkable pattern of criss-crossing linear features (faults, fault zones, arches) clustered into three major groups (N45/sup 0/-65/sup 0/W, N50/sup 0/-70/sup 0/E, E-W). The northwest- and northeast-trending faults are long-lived tectonic elements inherited from the Precambrian basement whose recurrent activity throughout the Phanerozoic strongly influenced sedimentation, facies distribution, and development of structures in the basin. Thermomechanical analyses indicate three main phases of subsidence (Silurian-Devonian, late Carboniferous-Permian, Late Jurassic-Early Cretaceous) and low geothermal gradients until the beginning of the Late Jurassic Permian oil-prone source rocks attained maturation due to extra heat originated from Juro-Cretaceous igneous intrusions. The third phase of subsidence also coincided with strong tectonic reactivation and creation of a third structural trend (east-west).

  9. Mechanisms of action of spa therapies in rheumatic diseases: what scientific evidence is there?

    PubMed

    Fioravanti, Antonella; Cantarini, Luca; Guidelli, Giacomo Maria; Galeazzi, Mauro

    2011-01-01

    Spa therapy represents a popular treatment for many rheumatic diseases. The mechanisms by which immersion in mineral or thermal water or the application of mud alleviates suffering in rheumatic diseases are not fully understood. The net benefit is probably the result of a combination of factors, with mechanical, thermal and chemical effects among the most prominent ones. Buoyancy, immersion, resistance and temperature all play important roles. According to the gate theory, pain relief may be due to the pressure and temperature of the water on skin; hot stimuli may influence muscle tone and pain intensity, helping to reduce muscle spasm and to increase the pain threshold. Mud-bath therapy increases plasma β-endorphin levels and secretion of corticotrophin, cortisol, growth hormone and prolactin. It has recently been demonstrated that thermal mud-pack therapy induces a reduction in the circulating levels of prostaglandin E2 (PGE2), leukotriene B4 (LTB4), interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α), important mediators of inflammation and pain. Spa therapy has been found to cause an increase in insulin-like growth factor-1 (IGF1), which stimulates cartilage metabolism, and transforming growth factor-β (TGF-β). There is also evidence of the positive action of mud-packs and thermal baths on the oxidant/antioxidant system, with a reduction in the release of reactive oxygen (ROS) and nitrogen (RNS) species. Overall, thermal stress has an immunosuppressive effect. Many other non-specific factors may also contribute to the beneficial effects observed after spa therapy in some rheumatic diseases, including effects on cardiovascular risk factors, and changes in the environment, pleasant surroundings and the absence of work duties.

  10. Age- and gender-associated Staphylococcus aureus spa types found among nasal carriers in a general population: the Tromso Staph and Skin Study.

    PubMed

    Sangvik, Maria; Olsen, Renate Slind; Olsen, Karina; Simonsen, Gunnar Skov; Furberg, Anne-Sofie; Sollid, Johanna U Ericson

    2011-12-01

    Staphylococcus aureus nasal carriers risk autoinfection; however, knowledge about the factors that make specific strains successful colonizers is limited. This study was undertaken to identify the most successful S. aureus clones in nasal carriers and compare their distribution among host groups. The population structure of S. aureus isolates from healthy adults was investigated by spa typing 1,981 isolates from persistent and intermittent nasal carriers participating in a health survey. In the baseline screening (1,113 isolates), the most common spa types were t012 (8.4%), t084 (7.6%), and t065 (4.9%). Three large spa clonal complexes (spa CC012, spa CC065, and spa CC084) comprised 62.4% of the isolates. In multivariate models adjusted for age and smoking status, male sex was associated with higher risk for spa type t084 (odds ratio [OR], 1.72; 95% confidence interval [CI], 1.06 to 2.77), and lower risk of spa type t012 (OR, 0.60; 95% CI, 0.39 to 0.92) colonization. The prevalence of spa type t012 decreased significantly with increasing age (P = 0.03), with a prevalence almost twice as high in the youngest group (age 30 to 44 years, prevalence = 11.1%) as in the oldest group (age, 60 to 87 years; prevalence = 5.6%). Among baseline isolates, spa type t084 had a twofold-higher prevalence among intermittent carriers than among persistent carriers (10.6% versus 5.5%; P = 0.04). In summary, the two most prevalent spa types found in this study were significantly associated with age and/or gender. This may provide valuable clues to the multifactorial mechanisms, among them bacterial factors, involved in nasal colonization with S. aureus.

  11. Ion-Molecule Reactions and Chemical Composition of Emanated from Herculane Spa Geothermal Sources

    PubMed Central

    Cosma, Constantin; Suciu, Ioan; Jäntschi, Lorentz; Bolboacă, Sorana D.

    2008-01-01

    The paper presents a chemical composition analysis of the gases emanated from geothermal sources in the Herculane Spa area (Romania). The upper homologues of methane have been identified in these gases. An ion-molecule reaction mechanism could be implicated in the formation of the upper homologues of methane. The CH4+ ions that appear under the action of radiation are the starting point of these reactions. The presence of hydrogen in the emanated gases may be also a result of these reactions. PMID:19325844

  12. SMART-1/Clementine study of Humorum and Procellarum Basins

    NASA Astrophysics Data System (ADS)

    Peters, Stefan

    We have combined SMART-1 AMIE camera images with Clementine UV/VIS data for two end-member lunar basins (circular - Mare Humorum and irregular - Oceanus Procellarum), to determine the coupling between basin origin, tectonics and volcanism. For Humorum, fault structures that include graben and strike-slip faults were mapped using ARC-GIS. Resulting tectonic maps agree well with an idealized model for mascon tectonics (Melosh 1978). This is in agreement with observed Bouguer anomalies. For Procellarum, there is no excess gravity and tectonic deformation does not agree with the Melosh model. We observed radial distribution of mare ridges in Humorum (1) and the presence of mare ridges without mascon tectonics in Procellarum (2), wherefor the origin of mare ridges in these areas cannot be related to mascons. To study impact related volcanic processes, craters with diameter ¡100 km were mapped and classified as volcanically flooded or unflooded. Our results suggest a mechanism in which craters were flooded related to large scale mare volcanic activity in the same or nearby area of the crater. We propose that this melt migrated to the specific craters through impact fractures. We used the Advanced Moon micro-Imager Experiment (AMIE) on ESA SMART-1 Moon mission, average pixel resolution of 80m. We also used UV/VIS pictures (multi-spectral images assessing the surface mineralogy of the Moon) and gravity maps derived after Clementine Moon mission (gravitational anomalies and crustal thickness maps). Ongoing research is taking place on the global structure and geochemical units of the South Pole-Aitken basin.

  13. Effectiveness of balneotherapy and spa therapy for the treatment of chronic low back pain: a review on latest evidence.

    PubMed

    Karagülle, Mine; Karagülle, Müfit Zeki

    2015-02-01

    In most European countries, balneotherapy and spa therapy are widely prescribed by physicians and preferred by European citizens for the treatment of musculoskeletal problems including chronic low back pain (LBP). We aimed to review and evaluate the recent evidence on the effectiveness of balneotherapy and spa therapy for patients with LBP. We comprehensively searched data bases for randomized controlled trials (RCTs) published in English between July 2005 and December 2013. We identified all trials testing balneotherapy or spa therapy for LBP that reported that the sequence of allocation was randomized. We finally included total of eight RCTs: two on balneotherapy and six on spa therapy. All reviewed trials reported that balneotherapy was superior in long term to tap water therapy in relieving pain and improving function and that spa therapy combining balneotherapy with mud pack therapy and/or exercise therapy, physiotherapy, and/or education was effective in the management of low back pain and superior or equally effective to the control treatments in short and long terms. We used Jadad scale to grade the methodological quality. Only three out of total eight had a score of above 3 indicating the good quality. The data from the RCTs indicates that overall evidence on effectiveness of balneotherapy and spa therapy in LBP is encouraging and reflects the consistency of previous evidence. However, the overall quality of trials is generally low. Better quality RCTs (well designed, conducted, and reported) are needed testing short- and long-term effects for relieving chronic back pain and proving broader beneficial effects.

  14. Activation of Histidine Kinase SpaK Is Mediated by the N-Terminal Portion of Subtilin-Like Lantibiotics and Is Independent of Lipid II.

    PubMed

    Spieß, Tobias; Korn, Sophie Marianne; Kötter, Peter; Entian, Karl-Dieter

    2015-08-15

    The biosynthesis of the lantibiotic subtilin is autoinduced in a quorum-sensing mechanism via histidine kinase SpaK. Subtilin-like lantibiotics, such as entianin, ericin S, and subtilin, specifically activated SpaK in a comparable manner, whereas the structurally similar nisin did not provide the signal for SpaK activation at nontoxic concentrations. Surprisingly, nevertheless, nisin if applied together with entianin partly quenched SpaK activation. The N-terminal entianin1-20 fragment (comprising N-terminal amino acids 1 to 20) was sufficient for SpaK activation, although higher concentrations were needed. The N-terminal nisin1-20 fragment also interfered with entianin-mediated activation of SpaK and, remarkably, at extremely high concentrations also activated SpaK. Our data show that the N-terminal entianin1-20 fragment is sufficient for SpaK activation. However, if present, the C-terminal part of the molecule further strongly enhances the activation, possibly by its interference with the cellular membrane. As shown by using lipid II-interfering substances and a lipid II-deficient mutant strain, lipid II is not needed for the sensing mechanism. PMID:26025904

  15. Activation of Histidine Kinase SpaK Is Mediated by the N-Terminal Portion of Subtilin-Like Lantibiotics and Is Independent of Lipid II

    PubMed Central

    Spieß, Tobias; Korn, Sophie Marianne

    2015-01-01

    The biosynthesis of the lantibiotic subtilin is autoinduced in a quorum-sensing mechanism via histidine kinase SpaK. Subtilin-like lantibiotics, such as entianin, ericin S, and subtilin, specifically activated SpaK in a comparable manner, whereas the structurally similar nisin did not provide the signal for SpaK activation at nontoxic concentrations. Surprisingly, nevertheless, nisin if applied together with entianin partly quenched SpaK activation. The N-terminal entianin1–20 fragment (comprising N-terminal amino acids 1 to 20) was sufficient for SpaK activation, although higher concentrations were needed. The N-terminal nisin1–20 fragment also interfered with entianin-mediated activation of SpaK and, remarkably, at extremely high concentrations also activated SpaK. Our data show that the N-terminal entianin1–20 fragment is sufficient for SpaK activation. However, if present, the C-terminal part of the molecule further strongly enhances the activation, possibly by its interference with the cellular membrane. As shown by using lipid II-interfering substances and a lipid II-deficient mutant strain, lipid II is not needed for the sensing mechanism. PMID:26025904

  16. 76 FR 53324 - Airworthiness Directives; Agusta S.p.A. Model A109A, A109A II, A109C, and A109K2 Helicopters

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-26

    .... Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February...-018-AD; Amendment 39-16765; AD 2011-17-01] RIN 2120-AA64 Airworthiness Directives; Agusta S.p.A. Model... airworthiness directive (EAD) for the specified Agusta S.p.A. (Agusta) model helicopters. That EAD...

  17. [Combined rehabilitative treatment of children with type 1 diabetes mellitus based at a spa and resort facility].

    PubMed

    Shashel', V A; Cherniak, I Iu

    2011-01-01

    A program of combined rehabilitative therapy of children and adolescents presenting with type 1 diabetes mellitus has been developed to be used under conditions of a spa and resort treatment. The program includes education in the "Diabetes mellitus management school", insulin therapy, an optimized regime of motor activity, nutritional therapy, thalassotherapy and heliotherapy, drinking of mineral water, balneo- and peloidotherapy, reflex-acupuncture therapy, and psychotherapy, underwater shower massage, enterosorption, and instrumental physiotherapy. The efficiency of the spa and resort rehabilitative treatment of the patients with type 1 diabetes mellitus was estimated at 93.2%. PMID:21988027

  18. Colonization of a therapeutic spa with Legionella spp: a public health issue.

    PubMed

    Costa, Joana; da Costa, Milton S; Veríssimo, António

    2010-01-01

    Traditional geothermally heated therapeutic spas, widely distributed in Europe, use water that is not disinfected. The colonization of therapeutic spas by Legionella spp. has been reported and several outbreaks and sporadic cases of legionellosis have been associated with the use of these facilities. We tested the effectiveness of hyperchlorination and the combination of hyperchlorination and ultraviolet light (UV) disinfection against legionellae which were persistently detected in the water used to supply the therapeutic spa. The hyperchlorination of the water distribution system was performed with a free residual chlorine concentration of 50 mg/l for 4h. An initial reduction in the numbers of legionellae was determined, but it rapidly increased to the original values. A UV unit was installed at the entrance to the spa's water distribution system and a new hyperchlorination procedure was performed immediately before UV irradiation (40 mJ/cm(2)), which was in use continuously for a nine-month period. While legionellae were not recovered during the irradiation period, these organisms were detected immediately after deactivation of the UV unit. We demonstrated that UV disinfection provides effective control of Legionella spp., with the advantage of being a method that, unlike chemical disinfectants, does not affect the physicochemical composition of the water. These findings suggest that UV irradiation, applied at key points in therapeutic spas, can be used to control colonization of water distribution systems by Legionella spp. PMID:20004717

  19. Are simultaneous postural adjustments (SPA) programmed as a function of pointing velocity?

    PubMed

    Fourcade, Paul; Le Bozec, Serge; Bouisset, Simon

    2016-10-01

    This paper deals with the influence of velocity on the postural adjustments that occur during the course of a voluntary movement, that is to say, simultaneous postural adjustments (SPA). To this aim, a pointing task performed at different velocities (V) was considered. Upper limb kinematics and body kinetics were recorded. Using a 2-DOF model, the body was divided into two parts: the right upper limb (termed the "focal" chain) and the rest of the body (termed the "postural" chain). This model allowed us to calculate the kinetics of both subsystems (-F x and [Formula: see text]), with one corresponding to the resultant action on the shoulder (AoSh: -F x) and the other to the resultant reaction of the shoulder (RoSh: [Formula: see text]). The influence of pointing velocity on peak amplitudes and durations was evaluated, as was their instantaneous relationship ("Lissajous ellipse"). The results showed that RoSh and AoSh display similar diphasic profiles, whose amplitude and duration vary with movement velocity. In addition, RoSh is in phase advance of AoSh, the advance being all the shorter as the focal movement velocity becomes faster. Finally, SPA appears to play a dual role, which includes a propulsive action during upper limb acceleration and body stabilization during deceleration. These new findings strengthen the hypothesis that the postural chain is programmed according to task velocity in the same way as the focal chain and that both are coping in order to make the task more efficient.

  20. Description of the behavior of an aquifer by using continuous radon monitoring in a thermal spa.

    PubMed

    Sainz, Carlos; Rábago, Daniel; Fuente, Ismael; Celaya, Santiago; Quindós, Luis Santiago

    2016-02-01

    Radon ((222)Rn) levels in air and water have been analyzed continuously for almost a year in Las Caldas de Besaya thermal spa, north Spain. Radon is a naturally occurring noble gas from the decay of radium ((226)Ra) both constituents of radioactive uranium 238 series. It has been recognized as a lung carcinogen by the World Health Organization (WHO) and International Agency for Research on Cancer (IARC). Furthermore the Royal Decree R.D 1439/2010 of November, 2010 establishes the obligation to study occupational activities where workers and, where appropriate, members of the public are exposed to inhalation of radon in workplaces such as spas. Together with radon measures several physico-chemical parameters were obtained such as pH, redox potential, electrical conductivity and air and water temperature. The devices used for the study of the temporal evolution of radon concentration have been the RTM 2100, the Radon Scout and gamma spectrometry was complementarily used to determine the transfer factor of the silicone tubes in the experimental device. Radon concentrations obtained in water and air of the spa are high, with an average of 660 Bq/l and 2900 Bq/m(3) respectively, where water is the main source of radon in the air. Radiation dose for workers and public was estimated from these levels of radon. The data showed that the thermal processes can control the behavior of radon which can be also influenced by various physical and chemical parameters such as pH and redox potential.

  1. Lunar Impact Basins: Stratigraphy, Sequence and Ages from Superposed Impact Crater Populations Measured from Lunar Orbiter Laser Altimeter (LOLA) Data

    NASA Technical Reports Server (NTRS)

    Fassett, C. I.; Head, J. W.; Kadish, S. J.; Mazarico, E.; Neumann, G. A.; Smith, D. E.; Zuber, M. T.

    2012-01-01

    Impact basin formation is a fundamental process in the evolution of the Moon and records the history of impactors in the early solar system. In order to assess the stratigraphy, sequence, and ages of impact basins and the impactor population as a function of time, we have used topography from the Lunar Orbiter Laser Altimeter (LOLA) on the Lunar Reconnaissance Orbiter (LRO) to measure the superposed impact crater size-frequency distributions for 30 lunar basins (D = 300 km). These data generally support the widely used Wilhelms sequence of lunar basins, although we find significantly higher densities of superposed craters on many lunar basins than derived by Wilhelms (50% higher densities). Our data also provide new insight into the timing of the transition between distinct crater populations characteristic of ancient and young lunar terrains. The transition from a lunar impact flux dominated by Population 1 to Population 2 occurred before the mid-Nectarian. This is before the end of the period of rapid cratering, and potentially before the end of the hypothesized Late Heavy Bombardment. LOLA-derived crater densities also suggest that many Pre-Nectarian basins, such as South Pole-Aitken, have been cratered to saturation equilibrium. Finally, both crater counts and stratigraphic observations based on LOLA data are applicable to specific basin stratigraphic problems of interest; for example, using these data, we suggest that Serenitatis is older than Nectaris, and Humboldtianum is younger than Crisium. Sample return missions to specific basins can anchor these measurements to a Pre-Imbrian absolute chronology.

  2. Comparison of automated repetitive-sequence-based polymerase chain reaction and spa typing versus pulsed-field gel electrophoresis for molecular typing of methicillin-resistant Staphylococcus aureus.

    PubMed

    Church, Deirdre L; Chow, Barbara L; Lloyd, Tracie; Gregson, Daniel B

    2011-01-01

    Automated repetitive polymerase chain reaction (PCR) (DiversiLab, bioMérieux, St. Laurent, Quebec, Canada) and single locus sequence typing of the Staphylococcus protein A (spa) gene with spa-type assignment by StaphType RIDOM software were compared to pulsed-field gel electrophoresis (PFGE) as the "gold standard" method for methicillin-resistant Staphylococcus aureus (MRSA) typing. Fifty-four MRSA isolates were typed by all methods: 10 of known PFGE CMRSA type and 44 clinical isolates. Correct assignment of CMRSA type or cluster occurred for 47 of 54 (87%) of the isolates when using a rep-PCR similarity index (SI) of ≥95%. Rep-PCR gave 7 discordant results [CMRSA1 (3), CMRSA2 (1), CMRSA4 (1), and CMRSA10 (2)], and some CMRSA clusters were not distinguished (CMRSA10/5/9, CMRSA 7/8, and CMRSA3/6). Several spa types occurred within a single PFGE or repetitive PCR types among the 19 different spa types found. spa type t037 was shared by CMRSA3 and CMRSA6 strains, and CMRSA9 and most CMRSA10 strains shared spa type t008. Time to results for PFGE, repetitive PCR, and spa typing was 3-4 days, 24 h, and 48 h, respectively. The annual costs of using spa or repetitive PCR were 2.4× and 1.9× higher, respectively, than PFGE but routine use of spa typing would lower annual labor costs by 0.10 full-time equivalents compared to PFGE. Repetitive PCR is a good method for rapid outbreak screening, but MRSA isolates that share the same repetitive PCR or PFGE patterns can be distinguished by spa typing.

  3. Lunar impact basins: New data for the nearside northern high latitudes and eastern limb from the second Galileo flyby

    NASA Technical Reports Server (NTRS)

    Head, J. W.; Belton, M.; Greeley, R.; Pieters, C.; Fischer, E.; Sunshine, J.; Klaasen, K.; Mcewen, A.; Becker, T.; Neukum, G.

    1993-01-01

    excavation and crustal stratigraphy, and assessment of models for basin formation and evolution. Here we discuss some preliminary results concerning lunar impact basins, their deposits, and prebasin substrates, using the same approaches that we employed for the Orientale and South Pole-Aitken basins using the data from the first encounter.

  4. Continental pollution in the western Mediterranean basin: vertical profiles of aerosol and trace gases measured over the sea during TRAQA 2012 and SAFMED 2013

    NASA Astrophysics Data System (ADS)

    Di Biagio, C.; Doppler, L.; Gaimoz, C.; Grand, N.; Ancellet, G.; Raut, J.-C.; Beekmann, M.; Borbon, A.; Sartelet, K.; Attié, J.-L.; Ravetta, F.; Formenti, P.

    2015-08-01

    In this study we present airborne observations of aerosol and trace gases obtained over the sea in the western Mediterranean basin during the TRAQA (TRansport and Air QuAlity) and SAFMED (Secondary Aerosol Formation in the MEDiterranean) campaigns in summer 2012 and 2013. A total of 23 vertical profiles were measured up to 5000 m above sea level over an extended area (40-45° N and 2° W-12° E) including the Gulf of Genoa, southern France, the Gulf of Lion, and the Spanish coast. During TRAQA and SAFMED the study area experienced a wide range of meteorological conditions which favoured pollution export from different sources located around the basin. Also, several events of dust outflows were measured during the campaigns. Observations from the present study show that continental pollution largely affects the western Mediterranean both close to coastal regions and in the open sea as far as ~ 250 km from the coastline. The measured aerosol scattering coefficient varies between ~ 20 and 120 Mm-1, while carbon monoxide (CO) and ozone (O3) mixing ratios are in the range of 60-165 and 30-85 ppbv, respectively. Pollution reaches 3000-4000 m in altitude and presents a very complex and highly stratified structure characterized by fresh and aged layers both in the boundary layer and in the free troposphere. Within pollution plumes the measured particle concentration in the Aitken (0.004-0.1 μm) and accumulation (0.1-1.0 μm) modes is between ~ 30 and 5000-6000 scm-3 (standard cm-3), which is comparable to the aerosol concentration measured in continental areas under pollution conditions. Additionally, our measurements indicate the presence of highly concentrated Aitken layers (10 000-15 000 scm-3) observed both close to the surface and in the free troposphere, possibly linked to the influence of new particle formation (NPF) episodes over the basin.

  5. Effect of spa therapy with saline balneotherapy on oxidant/antioxidant status in patients with rheumatoid arthritis: a single-blind randomized controlled trial

    NASA Astrophysics Data System (ADS)

    Karagülle, Mine; Kardeş, Sinan; Karagülle, Oğuz; Dişçi, Rian; Avcı, Aslıhan; Durak, İlker; Karagülle, Müfit Zeki

    2016-06-01

    Oxidative stress has been shown to play a contributory role in the pathogenesis of rheumatoid arthritis (RA). Recent studies have provided evidence for antioxidant properties of spa therapy. The purpose of this study is to investigate whether spa therapy with saline balneotherapy has any influence on the oxidant/antioxidant status in patients with RA and to assess clinical effects of spa therapy. In this investigator-blind randomized controlled trial, we randomly assigned 50 patients in a 1:1 ratio to spa therapy plus standard drug treatment (spa group) or standard drug treatment alone (control group). Spa group followed a 2-week course of spa therapy regimen consisting of a total of 12 balneotherapy sessions in a thermal mineral water pool at 36-37 °C for 20 min every day except Sunday. All clinical and biochemical parameters were assessed at baseline and after spa therapy (2 weeks). The clinical parameters were pain intensity, patient global assessment, physician global assessment, Health Assessment Questionnaire disability index (HAQ-DI), Disease Activity Score for 28-joints based on erythrocyte sedimentation rate (DAS28-4[ESR]). Oxidative status parameters were malondialdehyde (MDA), nonenzymatic superoxide radical scavenger activity (NSSA), antioxidant potential (AOP), and superoxide dismutase (SOD). The NSSA levels were increased significantly in the spa group (p = 0.003) but not in the control group (p = 0.509); and there was a trend in favor of spa therapy for improvements in NSSA levels compared to control (p = 0.091). Significant clinical improvement was found in the spa group compared to the control in terms of patient global assessment (p = 0.011), physician global assessment (p = 0.043), function (HAQ-DI) (p = 0.037), disease activity (DAS28-4[ESR]) (0.044) and swollen joint count (0.009), and a trend toward improvement in pain scores (0.057). Spa therapy with saline balneotherapy exerts antioxidant effect in patients with RA as reflected by the

  6. Surfactant proteins, SP-A and SP-D, in respiratory fungal infections: their role in the inflammatory response.

    PubMed

    Carreto-Binaghi, Laura Elena; Aliouat, El Moukhtar; Taylor, Maria Lucia

    2016-06-01

    Pulmonary surfactant is a complex fluid that comprises phospholipids and four proteins (SP-A, SP-B, SP-C, and SP-D) with different biological functions. SP-B, SP-C, and SP-D are essential for the lungs' surface tension function and for the organization, stability and metabolism of lung parenchyma. SP-A and SP-D, which are also known as pulmonary collectins, have an important function in the host's lung immune response; they act as opsonins for different pathogens via a C-terminal carbohydrate recognition domain and enhance the attachment to phagocytic cells or show their own microbicidal activity by increasing the cellular membrane permeability. Interactions between the pulmonary collectins and bacteria or viruses have been extensively studied, but this is not the same for fungal pathogens. SP-A and SP-D bind glucan and mannose residues from fungal cell wall, but there is still a lack of information on their binding to other fungal carbohydrate residues. In addition, both their relation with immune cells for the clearance of these pathogens and the role of surfactant proteins' regulation during respiratory fungal infections remain unknown. Here we highlight the relevant findings associated with SP-A and SP-D in those respiratory mycoses where the fungal infective propagules reach the lungs by the airways.

  7. 76 FR 7694 - Airworthiness Directives; PIAGGIO AERO INDUSTRIES S.p.A Model PIAGGIO P-180 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-11

    ..., amendment 39-16582 (76 FR 4056, January 24, 2011), currently requires an immediate functional test of the... INDUSTRIES S.p.A Model PIAGGIO P-180 Airplanes AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... Docket Management Facility between 9 a.m. and 5 p.m., Monday through Friday, except Federal holidays....

  8. 75 FR 43105 - Airworthiness Directives; PIAGGIO AERO INDUSTRIES S.p.A Model PIAGGIO P-180 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ... Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic... INDUSTRIES S.p.A Model PIAGGIO P-180 Airplanes AGENCY: Federal Aviation Administration (FAA), Department of...-140, 1200 New Jersey Avenue, SE., Washington, DC 20590, between 9 a.m. and 5 p.m., Monday...

  9. 75 FR 43095 - Airworthiness Directives; PIAGGIO AERO INDUSTRIES S.p.A. Model PIAGGIO P-180 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-23

    ... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... INDUSTRIES S.p.A. Model PIAGGIO P-180 Airplanes AGENCY: Federal Aviation Administration (FAA), Department of... failure of engine oil dipsticks, installed on Pratt & Whitney Canada (P&WC) PT6A66 and PT6A66B...

  10. 75 FR 68172 - Airworthiness Directives; Piaggio Aero Industries S.p.A. Model PIAGGIO P-180 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-05

    ... (75 FR 47734). That NPRM proposed to correct an unsafe condition for the specified products. The MCAI...'' under DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and (3) Will not have a... Industries S.p.A. Model PIAGGIO P-180 Airplanes AGENCY: Federal Aviation Administration (FAA), Department...

  11. 75 FR 5695 - Airworthiness Directives; PIAGGIO AERO INDUSTRIES S.p.A Model PIAGGIO P-180 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-04

    ... Register on November 30, 2009 (74 FR 62516). That NPRM proposed to correct an unsafe condition for the... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and (3) Will not have a significant... INDUSTRIES S.p.A Model PIAGGIO P-180 Airplanes AGENCY: Federal Aviation Administration (FAA), Department...

  12. 75 FR 47734 - Airworthiness Directives; Piaggio Aero Industries S.p.A. Model PIAGGIO P-180 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ... Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic... Industries S.p.A. Model PIAGGIO P-180 Airplanes AGENCY: Federal Aviation Administration (FAA), Department of...-140, 1200 New Jersey Avenue, SE., Washington, DC 20590, between 9 a.m. and 5 p.m., Monday...

  13. 76 FR 36980 - Airworthiness Directives; PIAGGIO AERO INDUSTRIES S.p.A Model PIAGGIO P-180 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-24

    ... . SUPPLEMENTARY INFORMATION: Airworthiness Directive 2011-09-51, Amendment 39-16697 (76 FR 27872, May 13, 2011... INDUSTRIES S.p.A Model PIAGGIO P-180 Airplanes AGENCY: Federal Aviation Administration (FAA), DOT. ACTION... 5 p.m., Monday through Friday, except Federal holidays. The AD docket contains this AD,...

  14. 75 FR 22512 - Airworthiness Directives; Piaggio Aero Industries S.p.A. Model PIAGGIO P-180 Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-29

    ... ] Register on February 19, 2010 (75 FR 7409). That NPRM proposed to correct an unsafe condition for the... Procedures (44 FR 11034, February 26, 1979); and (3) Will not have a significant economic impact, positive or... Industries S.p.A. Model PIAGGIO P-180 Airplanes AGENCY: Federal Aviation Administration (FAA), Department...

  15. Establishment and application of SPA-co-operated ELISA for detection of anti-HCV-IgM.

    PubMed

    Li, F H; Guo, L S; Yu, Z Q; Wang, Y K; Qi, J Y; Yuan, X W; Hao, L J

    1993-01-01

    A staphylococcus aureus protein A co-operated ELISA (SPA-ELISA) for the detection of anti-HCV-IgM has been established using HCV antigenic polypeptide, SPA-bearing germs and horseradish peroxidase labelled anti-human IgM. The specificity of SPA-ELISA has been confirmed by some substitution tests, blocking tests and destroying test with 2-mercaptoethanol. The results showed that the rate of anti-HCV-IgG in a group of patients with acute hepatitis and there were significant difference in anti-HCV-IgM was higher than that of anti-HCV-IgM detected rates between patients with acute hepatitis and those with chronic hepatitis (32.26%, P < 0.01). On the other hand, the positive rates of anti-HCV-IgM were 53.66% and 63.41% in transfusion associated hepatitis, 38.10% and 42.86% in sporadic hepatitis, 6.11% and 16.33% in people who have had active social activities, 40.00% and 10.00% in a group of blood donors respectively. Furthermore, taking into account the characteristics of HCV polypeptide used, its easiness of manipulation, and elimination of the interference of anti-HCV-IgG in sera, the new SPA-ELISA is believed to be of practical value in clinical and epidemiological studies of hepatitis C. PMID:7512150

  16. 75 FR 65263 - Virginia Graeme Baker Pool and Spa Safety Act; Public Accommodation; Withdrawal of Proposed Rule

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-22

    ... of such establishment as the residence of such proprietor'' (75 FR 12167). The Commission is... definition of ``public accommodations facility in the Federal Register of March 15, 2010 (75 FR 12167). The... COMMISSION 16 CFR Part 1450 Virginia Graeme Baker Pool and Spa Safety Act; Public Accommodation;...

  17. Association between the surfactant protein A (SP-A) gene locus and respiratory-distress syndrome in the Finnish population.

    PubMed Central

    Rämet, M; Haataja, R; Marttila, R; Floros, J; Hallman, M

    2000-01-01

    Respiratory-distress syndrome (RDS) in the newborn is a major cause of neonatal mortality and morbidity. Although prematurity is the most-important risk factor for RDS, the syndrome does not develop in many premature infants. The main cause of RDS is a deficiency of pulmonary surfactant, which consists of phospholipids and specific proteins. The genes underlying susceptibility to RDS are insufficiently known. The candidate-gene approach was used to study the association between the surfactant protein A (SP-A) gene locus and RDS in the genetically homogeneous Finnish population. In the present study, 88 infants with RDS and 88 control infants that were matched for degree of prematurity, prenatal glucocorticoid therapy, and sex were analyzed for SP-A genotypes. We show that certain SP-A1 alleles (6A2 and 6A3) and an SP-A1/SP-A2 haplotype (6A2/1A0) were associated with RDS. The 6A2 allele was overrepresented and the 6A3 allele was underrepresented in infants with RDS. These associations were particularly strong among small premature infants born at gestational age <32 wk. In infants protected from RDS (those that had no RDS, despite extreme prematurity and lack of glucocorticoid therapy), compared with infants that had RDS develop despite having received glucocorticoid therapy, the frequencies of 6A2 (.22 vs.71), 6A3 (.72 vs.17), 6A2/1A0 (.17 vs.68), 6A3/1A1 (.39 vs.10), and 6A3/1A2 (.28 vs.06) in the two groups, respectively, were strikingly different. According to the results of conditional logistic-regression analysis, diseases associated with premature birth did not explain the association between the odds of a particular homozygous SP-A1 genotype (6A2/6A2 and 6A3/6A3) and RDS. In the population evaluated in the present study, SP-B intron 4 variant frequencies were low and had no detectable association with RDS. We conclude that the SP-A gene locus is an important determinant for predisposition to RDS in premature infants. PMID:10762543

  18. The First Structure of a Lantibiotic Immunity Protein, SpaI from Bacillus subtilis, Reveals a Novel Fold*

    PubMed Central

    Christ, Nina A.; Bochmann, Sophie; Gottstein, Daniel; Duchardt-Ferner, Elke; Hellmich, Ute A.; Düsterhus, Stefanie; Kötter, Peter; Güntert, Peter; Entian, Karl-Dieter; Wöhnert, Jens

    2012-01-01

    Lantibiotics are peptide-derived antibiotics that inhibit the growth of Gram-positive bacteria via interactions with lipid II and lipid II-dependent pore formation in the bacterial membrane. Due to their general mode of action the Gram-positive producer strains need to express immunity proteins (LanI proteins) for protection against their own lantibiotics. Little is known about the immunity mechanism protecting the producer strain against its own lantibiotic on the molecular level. So far, no structures have been reported for any LanI protein. We solved the structure of SpaI, a LanI protein from the subtilin producing strain Bacillus subtilis ATCC 6633. SpaI is a 16.8-kDa lipoprotein that is attached to the outside of the cytoplasmic membrane via a covalent diacylglycerol anchor. SpaI together with the ABC transporter SpaFEG protects the B. subtilis membrane from subtilin insertion. The solution-NMR structure of a 15-kDa biologically active C-terminal fragment reveals a novel fold. We also demonstrate that the first 20 N-terminal amino acids not present in this C-terminal fragment are unstructured in solution and are required for interactions with lipid membranes. Additionally, growth tests reveal that these 20 N-terminal residues are important for the immunity mediated by SpaI but most likely are not part of a possible subtilin binding site. Our findings are the first step on the way of understanding the immunity mechanism of B. subtilis in particular and of other lantibiotic producing strains in general. PMID:22904324

  19. The Successive Projection Algorithm (SPA), an Algorithm with a Spatial Constraint for the Automatic Search of Endmembers in Hyperspectral Data

    PubMed Central

    Zhang, Jinkai; Rivard, Benoit; Rogge, D.M.

    2008-01-01

    Spectral mixing is a problem inherent to remote sensing data and results in few image pixel spectra representing ″pure″ targets. Linear spectral mixture analysis is designed to address this problem and it assumes that the pixel-to-pixel variability in a scene results from varying proportions of spectral endmembers. In this paper we present a different endmember-search algorithm called the Successive Projection Algorithm (SPA). SPA builds on convex geometry and orthogonal projection common to other endmember search algorithms by including a constraint on the spatial adjacency of endmember candidate pixels. Consequently it can reduce the susceptibility to outlier pixels and generates realistic endmembers.This is demonstrated using two case studies (AVIRIS Cuprite cube and Probe-1 imagery for Baffin Island) where image endmembers can be validated with ground truth data. The SPA algorithm extracts endmembers from hyperspectral data without having to reduce the data dimensionality. It uses the spectral angle (alike IEA) and the spatial adjacency of pixels in the image to constrain the selection of candidate pixels representing an endmember. We designed SPA based on the observation that many targets have spatial continuity (e.g. bedrock lithologies) in imagery and thus a spatial constraint would be beneficial in the endmember search. An additional product of the SPA is data describing the change of the simplex volume ratio between successive iterations during the endmember extraction. It illustrates the influence of a new endmember on the data structure, and provides information on the convergence of the algorithm. It can provide a general guideline to constrain the total number of endmembers in a search.

  20. [Practicing subnarcotic xenon dose inhalation in spa treatment of posttraumatic stress-induced disorders].

    PubMed

    Igoshina, T V; Kotrovskaya, T I; Bubeev, Yu A; Schastlivtseva, D V; Potapov, A V

    2014-01-01

    Purpose of the investigation was to compare and contrast effectiveness of xenon therapy of stress-induced neurotic disorders and traditional spa-based therapy. Patients of the experimental and control groups were people of risky professions who received drug therapy, psychotherapy and physiotherapy. The experimental group was additionally treated by inhalation therapeutic doses of medical xenon. Comparative analysis of qualitative and quantitative parameters of electroencephalogram (EEG), blood oxygen level, heart rate and blood pressure were compared in the groups before and after treatment. Recovery of the central nervous system functions, activation of parasympathetic involvement, abatement of main psychopathological and somatovegetative disorders in the experimental group were considered as signs of psychic improvement and return to the gestalt behavior. PMID:26036001

  1. Single Piezo-Actuator Rotary-Hammering (SPaRH) Drill

    NASA Technical Reports Server (NTRS)

    Sherrit, Stewart (Inventor); Bao, Xiaoqi (Inventor); Badescu, Mircea (Inventor); Bar-Cohen, Yoseph (Inventor)

    2014-01-01

    A Single Piezo-Actuator Rotary-Hammering (SPaRH) Drill includes a horn actuator having high power piezoelectric materials and a flexure pre-stress to increase the actuators effectiveness. The drill is a low mass, low power, compact coring drill measuring 20-cm high by 7-cm diameter and having a total weight of 2 kg including drive electronics. Using an average power of 50-Watts, the drill basalt is expected to cut basalt at a rate of 0.2 cm/min down to depth of 10-cm and create cuttings and an intact core. The drill is expected to operate under different environments including Martian ambient (6 Torr and down to -50 degree C), and liquid nitrogen temperatures (77 K) and low pressure (<<1 Torr) to simulate lunar polar and Europa conditions. Materials expected to be sampled include Kaolinite, Saddleback Basalt, Limestone, Volcanic Breccia, Siltstone, ice, permafrost and layered rocks with different hardness.

  2. The synthetic ajoene analog SPA3015 induces apoptotic cell death through crosstalk between NF-κB and PPARγ in multidrug-resistant cancer cells.

    PubMed

    Hwang, Jee Won; Cho, Hyewon; Lee, Jae Yeon; Jeon, Youngsic; Kim, Su-Nam; Lee, Sang Jin; Bae, Gyu-Un; Yoon, Sungpil; Jeon, Raok; Kim, Yong Kee

    2016-10-01

    Multidrug resistance (MDR) caused by P-glycoprotein (P-gp) overexpression impedes successful cancer chemotherapy. In this study, we investigated the anticancer effects of SPA3015, a synthetic ajoene analog, in P-gp-overexpressing MDR cancer cells (KBV20C and MES-SA/DX5). Treatment with SPA3015 caused a dramatic decrease in the cell viabilities of both KBV20C and MES-SA/DX5 cells. This decrease was accompanied by apoptotic cell death without affecting the expression level or drug efflux function of P-gp. SPA3015 selectively suppressed NF-κB reporter gene activity, which led to decreased expression of NF-κB target genes such as CIAP1, CIAP2, XIAP, and Bcl-XL. Surprisingly, nuclear localization and DNA binding affinity of the p65 subunit were not affected by SPA3015, suggesting that SPA3015 inhibits the transcriptional activity of NF-κB at the nucleus. Indeed, SPA3015 treatment led to an increase in the physical interaction of p65 with PPARγ, which resulted in the inhibition of NF-κB activity. Our findings support the hypothesis that SPA3015 inhibits NF-κB transcriptional activity by facilitating the physical interaction of the p65 subunit and PPARγ, which leads to apoptotic cell death in MDR cancer cells.

  3. Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection.

    PubMed

    Khrustalev, Vladislav Victorovich; Ghaznavi-Rad, Ehsanollah; Neela, Vasanthakumari; Shamsudin, Mariana-Nor; Amouzandeh-Nobaveh, Alireza; Barkovsky, Eugene Victorovich

    2013-11-01

    Fifteen sequences with stop codons have been obtained in the course of standard methicillin-resistant Staphylococcus aureus (MRSA) spa typing. In nine of those sequences, stop codons occurred due to nonsense G-T and A-T transversions. G-T transversions would appear to be frequent in the spa gene, mostly due to symmetric mutational AT-pressure in the whole S. aureus genome and due to replication-associated mutational pressure characteristic of lagging strands of the "chromosome". A-T transversions would appear to be frequent in the spa gene mostly due to transcription-associated mutational pressure. Relative to other S. aureus genes, short repeats in spa are enriched by nonsense sites for G-T and A-T transversions; the probability of being nonsense for A-T transversion is high in that part of spa coding region. 13 out of 15 (87%) of the sequences with stop codons were obtained from strains isolated from patients with generalized S. aureus infection. Truncation of spa at its C-terminus is predicted to result in a protein that possesses functional IgG binding domains unable to be linked to the cell wall. This is discussed in light of the known fact that extracellular spa is a strong virulence factor involved in immune evasion.

  4. Description of the behavior of an aquifer by using continuous radon monitoring in a thermal spa.

    PubMed

    Sainz, Carlos; Rábago, Daniel; Fuente, Ismael; Celaya, Santiago; Quindós, Luis Santiago

    2016-02-01

    Radon ((222)Rn) levels in air and water have been analyzed continuously for almost a year in Las Caldas de Besaya thermal spa, north Spain. Radon is a naturally occurring noble gas from the decay of radium ((226)Ra) both constituents of radioactive uranium 238 series. It has been recognized as a lung carcinogen by the World Health Organization (WHO) and International Agency for Research on Cancer (IARC). Furthermore the Royal Decree R.D 1439/2010 of November, 2010 establishes the obligation to study occupational activities where workers and, where appropriate, members of the public are exposed to inhalation of radon in workplaces such as spas. Together with radon measures several physico-chemical parameters were obtained such as pH, redox potential, electrical conductivity and air and water temperature. The devices used for the study of the temporal evolution of radon concentration have been the RTM 2100, the Radon Scout and gamma spectrometry was complementarily used to determine the transfer factor of the silicone tubes in the experimental device. Radon concentrations obtained in water and air of the spa are high, with an average of 660 Bq/l and 2900 Bq/m(3) respectively, where water is the main source of radon in the air. Radiation dose for workers and public was estimated from these levels of radon. The data showed that the thermal processes can control the behavior of radon which can be also influenced by various physical and chemical parameters such as pH and redox potential. PMID:26599146

  5. State-wide surveillance of antibiotic resistance patterns and spa types of methicillin-resistant Staphylococcus aureus from blood cultures in North Rhine-Westphalia, 2011-2013.

    PubMed

    Cuny, C; Layer, F; Werner, G; Harmsen, D; Daniels-Haardt, I; Jurke, A; Mellmann, A; Witte, W; Köck, R

    2015-08-01

    Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of bacteraemia. We aimed to obtain a complete picture of severe MRSA infections by characterizing all MRSA isolates from bloodstream infections in the largest German federal state (North Rhine-Westphalia, 18 million inhabitants) using S. aureus protein A (spa) sequence-typing and antimicrobial susceptibility testing. MRSA isolates (n = 1952) were collected prospectively (2011-2013) and spa-typed. Among 181 different spa types, t003 (n = 746 isolates; 38.2%) and t032 (n = 594; 30.4%) were predominant. Analysis of the geographical occurrence of spa clonal complexes (spa-CCs) and spa types revealed divergent distribution between federal state districts for spa-CCs 003 (p < 0.001; including t003, p < 0.001 and t264, p < 0.001), 008 (p 0.021), 011 (p 0.002), 032 (p < 0.001; including t022, p 0.014 and t032, p < 0.001) and spa type t2807 (p < 0.001). MICs of antimicrobial substances were tested using broth microdilution. Of all isolates, 96% were resistant to fluoroquinolones, 78% to erythromycin, 70% to clindamycin, 4% to gentamicin, 2% to rifampicin, 0.4% to daptomycin, 0.1% to linezolid and 0% to vancomycin, respectively. Vancomycin MICs of 2 mg/L involved 0.5% of the isolates. In conclusion, the detection of regional molecular clusters added valuable information for epidemiological case tracing and allowed conclusions to be reached on the importance of newly emerging MRSA reservoirs, such as livestock (spa-CC011), for MRSA bacteraemia in some parts of the federal state. Susceptibility testing revealed broad resistance to substances used for oral treatment, but demonstrated that those antibiotics that are mostly applied for treatment of MRSA bacteraemia and important combination partners were highly susceptible.

  6. Enhanced discrimination of highly clonal ST22-methicillin-resistant Staphylococcus aureus IV isolates achieved by combining spa, dru, and pulsed-field gel electrophoresis typing data.

    PubMed

    Shore, Anna C; Rossney, Angela S; Kinnevey, Peter M; Brennan, Orla M; Creamer, Eilish; Sherlock, Orla; Dolan, Anthony; Cunney, Robert; Sullivan, Derek J; Goering, Richard V; Humphreys, Hilary; Coleman, David C

    2010-05-01

    ST22-methicillin-resistant Staphylococcus aureus type IV (ST22-MRSA-IV) is endemic in Irish hospitals and is designated antibiogram-resistogram type-pulsed-field group (AR-PFG) 06-01. Isolates of this highly clonal strain exhibit limited numbers of pulsed-field gel electrophoresis (PFGE) patterns and spa types. This study investigated whether combining PFGE and spa typing with DNA sequencing of the staphylococcal cassette chromosome mec element (SCCmec)-associated direct repeat unit (dru typing) would improve isolate discrimination. A total of 173 MRSA isolates recovered in one Irish hospital during periods in 2007 and 2008 were investigated using antibiogram-resistogram (AR), PFGE, spa, dru, and SCCmec typing. Isolates representative of each of the 17 pulsed-field group 01 (PFG-01) spa types identified underwent multilocus sequence typing, and all isolates were ST22. Ninety-seven percent of isolates (168 of 173) exhibited AR-PFG 06-01 or closely related AR patterns, and 163 of these isolates harbored SCCmec type IVh. The combination of PFGE, spa, and dru typing methods significantly improved discrimination of the 168 PFG-01 isolates, yielding 65 type combinations with a Simpson's index of diversity (SID) of 96.53, compared to (i) pairwise combinations of spa and dru typing, spa and PFGE typing, and dru and PFGE typing, which yielded 37, 44, and 43 type combinations with SIDs of 90.84, 91.00, and 93.57, respectively, or (ii) individual spa, dru, and PFGE typing methods, which yielded 17, 17, and 21 types with SIDs of 66.9, 77.83, and 81.34, respectively. Analysis of epidemiological information for a subset of PFG-01 isolates validated the relationships inferred using combined PFGE, spa, and dru typing data. This approach significantly enhances discrimination of ST22-MRSA-IV isolates and could be applied to epidemiological investigations of other highly clonal MRSA strains.

  7. Surfactant Protein A (SP-A)-mediated Clearance of Staphylococcus aureus Involves Binding of SP-A to the Staphylococcal Adhesin Eap and the Macrophage Receptors SP-A Receptor 210 and Scavenger Receptor Class A*

    PubMed Central

    Sever-Chroneos, Zvjezdana; Krupa, Agnieszka; Davis, Jeremy; Hasan, Misbah; Yang, Ching-Hui; Szeliga, Jacek; Herrmann, Mathias; Hussain, Muzafar; Geisbrecht, Brian V.; Kobzik, Lester; Chroneos, Zissis C.

    2011-01-01

    Staphylococcus aureus causes life-threatening pneumonia in hospitals and deadly superinfection during viral influenza. The current study investigated the role of surfactant protein A (SP-A) in opsonization and clearance of S. aureus. Previous studies showed that SP-A mediates phagocytosis via the SP-A receptor 210 (SP-R210). Here, we show that SP-R210 mediates binding and control of SP-A-opsonized S. aureus by macrophages. We determined that SP-A binds S. aureus through the extracellular adhesin Eap. Consequently, SP-A enhanced macrophage uptake of Eap-expressing (Eap+) but not Eap-deficient (Eap−) S. aureus. In a reciprocal fashion, SP-A failed to enhance uptake of Eap+ S. aureus in peritoneal Raw264.7 macrophages with a dominant negative mutation (SP-R210(DN)) blocking surface expression of SP-R210. Accordingly, WT mice cleared infection with Eap+ but succumbed to sublethal infection with Eap- S. aureus. However, SP-R210(DN) cells compensated by increasing non-opsonic phagocytosis of Eap+ S. aureus via the scavenger receptor scavenger receptor class A (SR-A), while non-opsonic uptake of Eap− S. aureus was impaired. Macrophages express two isoforms: SP-R210L and SP-R210S. The results show that WT alveolar macrophages are distinguished by expression of SP-R210L, whereas SR-A−/− alveolar macrophages are deficient in SP-R210L expressing only SP-R210S. Accordingly, SR-A−/− mice were highly susceptible to both Eap+ and Eap− S. aureus. The lungs of susceptible mice generated abnormal inflammatory responses that were associated with impaired killing and persistence of S. aureus infection in the lung. In conclusion, alveolar macrophage SP-R210L mediates recognition and killing of SP-A-opsonized S. aureus in vivo, coordinating inflammatory responses and resolution of S. aureus pneumonia through interaction with SR-A. PMID:21123169

  8. Lunar Basins: New Evidence from Gravity for Impact-Formed Mascons

    NASA Technical Reports Server (NTRS)

    Neumann, Gregory A.; Lemoine, F. G.; Smith, D. E.; Zuber, M. T.

    1998-01-01

    timescales has erased some but not all of the signature of the impact process. Mantle uplift inferred from gravity modeling is inversely correlated with age. While the oldest basins such as South Pole Aitken are mainly compensated isotatically, the younger basins appear to have been in a state of superisostatic loading prior to mare emplacement. If this is true, this places an important constraint on the impact process at basin scales. The idea that rebound of the transient crater via acoustic fluidization may freeze substantial stresses imcomplete to this day, may be tested by examining the gravity signatures of major basins on terrestrial bodies. The moon provides the clearest resolved examples to date, but uncertainty in gravity knowledge remains problematic.

  9. Conversion from CUL4-based COP1-SPA E3 apparatus to UVR8-COP1-SPA complexes underlies a distinct biochemical function of COP1 under UV-B.

    PubMed

    Huang, Xi; Ouyang, Xinhao; Yang, Panyu; Lau, On Sun; Chen, Liangbi; Wei, Ning; Deng, Xing Wang

    2013-10-01

    The evolutionarily conserved constitutive photomorphogenesis 1 (COP1) is a RING and WD40 protein that functions as a substrate receptor of CULLIN4-damaged DNA binding protein 1 (CUL4-DDB1)-based E3 ubiquitin ligases in both plants and animals. In Arabidopsis, COP1 is a central repressor of photomorphogenesis in the form of COP1-suppressor of PHYA (SPA) complex(es). CUL4-DDB1-COP1-SPA suppresses the photomorphogenic program by targeting the transcription factor elongated hypocotyl 5 for degradation. Intriguingly, under photomorphogenic UV-B light, COP1 reverses its repressive role and promotes photomorphogenesis. However, the mechanism by which COP1 is functionally switched is still obscure. Here, we demonstrate that UV-B triggers the physical and functional disassociation of the COP1-SPA core complex(es) from CUL4-DDB1 and the formation of a unique complex(es) containing the UV-B receptor UV resistance locus 8 (UVR8). The establishment of this UV-B-dependent COP1 complex(es) is associated with its positive modulation of elongated hypocotyl 5 stability and activity, which sheds light on the mechanism of COP1's promotive action in UV-B-induced photomorphogenesis.

  10. [The prospects for the further development of the Belokurikha spa and health resort territory in the Altai Krai (region)].

    PubMed

    Dzhabarova, N K; Yakovenko, E S; Sidorina, N G; Kokhanenko, A A; Vorob'ev, V A; Zaitsev, A A; Kovalenko, T S; Zhilyakov, I V

    2016-01-01

    We undertook a balneological survey of the Belokurikha spa and health resort territory with the purpose of distinguishing and identifying the potential health-improvement areas most promising for the extension and optimization of the therapeutic, tourist and recreational activities. The assessment was focused on the characteristic of the landscape and climatic conditions of the territory, the possibilities for the development of the existing resources of mineral waters and therapeutic muds as well as for the discovery of the potential new ones. The recommendations are proposed to promote the development of different forms of tourism with special reference to its medical and health-improvement aspects. It is suggested that the territory of the «Belokurikha» resort», «Belokurikha-2» and «Belokurikha-3» health-improvement areas should be integrated into a single spa-and-health resort district of federal importance.

  11. [The prospects for the further development of the Belokurikha spa and health resort territory in the Altai Krai (region)].

    PubMed

    Dzhabarova, N K; Yakovenko, E S; Sidorina, N G; Kokhanenko, A A; Vorob'ev, V A; Zaitsev, A A; Kovalenko, T S; Zhilyakov, I V

    2016-01-01

    We undertook a balneological survey of the Belokurikha spa and health resort territory with the purpose of distinguishing and identifying the potential health-improvement areas most promising for the extension and optimization of the therapeutic, tourist and recreational activities. The assessment was focused on the characteristic of the landscape and climatic conditions of the territory, the possibilities for the development of the existing resources of mineral waters and therapeutic muds as well as for the discovery of the potential new ones. The recommendations are proposed to promote the development of different forms of tourism with special reference to its medical and health-improvement aspects. It is suggested that the territory of the «Belokurikha» resort», «Belokurikha-2» and «Belokurikha-3» health-improvement areas should be integrated into a single spa-and-health resort district of federal importance. PMID:27213948

  12. Rapid and high-resolution distinction of community-acquired and nosocomial Staphylococcus aureus isolates with identical pulsed-field gel electrophoresis patterns and spa types.

    PubMed

    Glasner, Corinna; Sabat, Artur J; Dreisbach, Annette; Larsen, Anders R; Friedrich, Alexander W; Skov, Robert L; van Dijl, Jan Maarten

    2013-03-01

    Methicillin-resistant Staphylococcus aureus (MRSA) represent a serious threat for public health worldwide. Of particular concern is the emergence of community-acquired MRSA, which is often difficult to distinguish from nosocomial MRSA due to a lack of suitable typing methods for early detection. For example, the USA300 pulsed-field gel electrophoresis (PFGE) pattern includes both the 'classical' community-acquired USA300 clone with spa type t008 and an epidemiologically unrelated nosocomial clone with spa type t024. Likewise, spa typing cannot distinguish the classic USA300 from nosocomial MRSA with the spa type t008. Since the fast and high-resolution distinction of these S. aureus types is important for infection prevention and surveillance, we investigated whether multiple-locus variable number tandem repeat fingerprinting (MLVF) can be applied to overcome these limitations. Indeed, MLVF correctly grouped 91 MRSA isolates belonging to the classic USA300 lineage, nosocomial MRSA isolates with the USA300 PFGE profile and spa type t024, and nosocomial MRSA isolates with spa type t008 into 3 distinct clusters. Importantly, several sub-clusters were also identified, reflecting epidemiological relationships between the respective isolates. We conclude that MLVF has the discriminatory power needed to rapidly distinguish very similar community-acquired and nosocomial MRSA isolates and that MLVF-based sub-clustering of isolates is highly useful for epidemiological investigations, outbreak prevention, and control.

  13. New insights about pilus formation in gut-adapted Lactobacillus rhamnosus GG from the crystal structure of the SpaA backbone-pilin subunit

    PubMed Central

    Chaurasia, Priyanka; Pratap, Shivendra; von Ossowski, Ingemar; Palva, Airi; Krishnan, Vengadesan

    2016-01-01

    Thus far, all solved structures of pilin-proteins comprising sortase-assembled pili are from pathogenic genera and species. Here, we present the first crystal structure of a pilin subunit (SpaA) from a non-pathogen host (Lactobacillus rhamnosus GG). SpaA consists of two tandem CnaB-type domains, each with an isopeptide bond and E-box motif. Intriguingly, while the isopeptide bond in the N-terminal domain forms between lysine and asparagine, the one in the C-terminal domain atypically involves aspartate. We also solved crystal structures of mutant proteins where residues implicated in forming isopeptide bonds were replaced. Expectedly, the E-box-substituted E139A mutant lacks an isopeptide bond in the N-terminal domain. However, the C-terminal E269A substitution gave two structures; one of both domains with their isopeptide bonds present, and another of only the N-terminal domain, but with an unformed isopeptide bond and significant conformational changes. This latter crystal structure has never been observed for any other Gram-positive pilin. Notably, the C-terminal isopeptide bond still forms in D295N-substituted SpaA, irrespective of E269 being present or absent. Although E-box mutations affect SpaA proteolytic and thermal stability, a cumulative effect perturbing normal pilus polymerization was unobserved. A model showing the polymerized arrangement of SpaA within the SpaCBA pilus is proposed. PMID:27349405

  14. Surfactant Proteins SP-A and SP-D Modulate Uterine Contractile Events in ULTR Myometrial Cell Line

    PubMed Central

    Sotiriadis, Georgios; Dodagatta-Marri, Eswari; Kouser, Lubna; Alhamlan, Fatimah S.; Kishore, Uday; Karteris, Emmanouil

    2015-01-01

    Pulmonary surfactant proteins SP-A and SP-D are pattern recognition innate immune molecules. However, there is extrapulmonary existence, especially in the amniotic fluid and at the feto-maternal interface. There is sufficient evidence to suggest that SP-A and SP-D are involved in the initiation of labour. This is of great importance given that preterm birth is associated with increased mortality and morbidity. In this study, we investigated the effects of recombinant forms of SP-A and SP-D (rhSP-A and rhSP-D, the comprising of trimeric lectin domain) on contractile events in vitro, using a human myometrial cell line (ULTR) as an experimental model. Treatment with rhSP-A or rhSP-D increased the cell velocity, distance travelled and displacement by ULTR cells. rhSP-A and rhSP-D also affected the contractile response of ULTRs when grown on collagen matrices showing reduced surface area. We investigated this effect further by measuring contractility-associated protein (CAP) genes. Treatment with rhSP-A and rhSP-D induced expression of oxytocin receptor (OXTR) and connexin 43 (CX43). In addition, rhSP-A and rhSP-D were able to induce secretion of GROα and IL-8. rhSP-D also induced the expression of IL-6 and IL-6 Ra. We provide evidence that SP-A and SP-D play a key role in modulating events prior to labour by reconditioning the human myometrium and in inducing CAP genes and pro-inflammatory cytokines thus shifting the uterus from a quiescent state to a contractile one. PMID:26641881

  15. [Marketing macroenvironment of a sanatorium and spa facility as a study object and a factor of strategic decision making].

    PubMed

    Babaskin, D V

    2009-01-01

    Analysis of marketing macroenvironment is an important stage in the formulation of the strategy for the development and operation of a therapeutic and preventive healthcare facility making possible rapid adjustment to sharply changing marketing conditions. The possibility of using expert evaluation for the analysis of the marketing macroenvironment of a sanatorium and spa facility and a concrete medical care service provided (peloidotherapy) is illustrated. The study covers five aspects of marketing macroenvironment, viz. socio-demographic, technological, economic, political and ecological. PMID:19637841

  16. spa Typing and Multilocus Sequence Typing Show Comparable Performance in a Macroepidemiologic Study of Staphylococcus aureus in the United States.

    PubMed

    O'Hara, F Patrick; Suaya, Jose A; Ray, G Thomas; Baxter, Roger; Brown, Megan L; Mera, Robertino M; Close, Nicole M; Thomas, Elizabeth; Amrine-Madsen, Heather

    2016-01-01

    A number of molecular typing methods have been developed for characterization of Staphylococcus aureus isolates. The utility of these systems depends on the nature of the investigation for which they are used. We compared two commonly used methods of molecular typing, multilocus sequence typing (MLST) (and its clustering algorithm, Based Upon Related Sequence Type [BURST]) with the staphylococcal protein A (spa) typing (and its clustering algorithm, Based Upon Repeat Pattern [BURP]), to assess the utility of these methods for macroepidemiology and evolutionary studies of S. aureus in the United States. We typed a total of 366 clinical isolates of S. aureus by these methods and evaluated indices of diversity and concordance values. Our results show that, when combined with the BURP clustering algorithm to delineate clonal lineages, spa typing produces results that are highly comparable with those produced by MLST/BURST. Therefore, spa typing is appropriate for use in macroepidemiology and evolutionary studies and, given its lower implementation cost, this method appears to be more efficient. The findings are robust and are consistent across different settings, patient ages, and specimen sources. Our results also support a model in which the methicillin-resistant S. aureus (MRSA) population in the United States comprises two major lineages (USA300 and USA100), which each consist of closely related variants.

  17. [Legionella pneumophila serogroup 3 isolated from a patient of pneumonia developed after drowning in bathtub of a hot spring spa].

    PubMed

    Shiota, R; Takeshita, K; Yamamoto, K; Imada, K; Yabuuchi, E; Wang, L

    1995-12-01

    A 71-year-old Japanese female, was found unconscious by drawing, in a hot spring spa, at around noon of 20 October 1994. She recovered by emergency cardiopulmonary resuscitation, and admitted to the Takinomiya General Hospital, with adult respiratory distress syndrome (ARDS). Although she recovered from ARDS within 4 days after her admission, she developed severe pneumonia accompanied with the second attack of ARDS. Ordinary bacteriological culture of her respiratory specimens failed to yield any significant pathogen for her pneumonia, and neither cefazolin nor imipenem/cilastatin was effective. Thus minocyclin was given on the 7th hospital-day and this was effective for blood gas and C-reactive protein (CRP) levels. Intratracheal exsudate inoculated on BCYE alpha agar plate yielded grayish white colonies. Cells of the colonies were clearly agglutinated by anti-Legionella pneumophila serogroup (SG) 3 serum. Antibody titers of patient's paired sera against the strain L. pneumophila SG3 Bloomington-2 and the patient's strain (Y-1) were determined by microplate agglutination test, and a significant rise from 1:20 to 1:320 was demonstrated. Patient recovered by erythromycin treatment and was discharged on the 59th hospital day. L. pneumophila SG3 organisms were again isolated from the spa water where the patient drawn. From these findings described above, we diagnosed the patient as pneumonia due to L. pneumophila SG3, and the spa water was the most probable source of infection.

  18. The Mechanics of Impact Basin Formation: Comparisons between Modeling and Geophysical Observations

    NASA Astrophysics Data System (ADS)

    Stewart, S. T.

    2010-12-01

    major ring structures (derived from topography and geologic mapping) and the crustal thickness and mantle topography (derived from gravity and topography) [see also 3]. Preliminary comparisons indicate that the simulations produce the major features in the observations. I will present detailed comparisons between simulations and observations for major basins on the moon, including South Pole-Aitken, for different initial lithospheric thicknesses and thermal gradients. [1] Spudis, P.D. (1993) The Geology of Multi-Ring Impact basins: Cambridge University Press. [2] Senft, L.E. and S.T. Stewart (2009) Earth and Planetary Science Letters 287, 471-482. [3] Lillis, R.J., et al. (2010) AGU Fall Meeting.

  19. The development of selected cardiovascular parameters in patients with type 2 diabetes mellitus during a spa treatment.

    PubMed

    Fialová, E; Kittnar, O

    2015-01-01

    Diabetes mellitus is not just a simple metabolic disorder, however, it is considered to be a cardiovascular disease of a metabolic origin. This is apparent especially when speaking about type 2 diabetes (DM II). The objective of our study was to determine whether a comprehensive spa treatment (procedures and drinking cure) may affect the level of the sympathetic tone of patients suffering from DM II. As an indicator of the sympathetic tone, selected electrocardiographic parameters derived from the heart rate variability and microwave alternans were chosen. There were 96 patients enrolled in our study: 38 patients with poorly controlled DM II and two control groups: 9 patients with compensated DM II and 49 patients, average age without diabetes or other disorders of the glucose metabolism. All received an identical spa treatment and continued their medical therapy. The electrophysiological examination of patients was performed before and after a three-week spa treatment using the KARDiVAR system. Parameters derived from the analysis of heart rate variability (HRV), microvolt T-wave alternans, and microvolt R-wave alternans were analyzed in order to evaluate the tones of the autonomic nervous system (ANS). The control group showed a slight increase of parameter the index of activity of regulatory systems (IRSA) (4.4+/-1.3 vs. 3.8+/-1.4; p=0.006) after the spa treatment, while increased heart rate (80.9+/-11.0 vs. 74.6+/-9.6; p=0.028), reduced index of centralization (IC) (1.3+/-0.6 vs. 2.9+/-1.4; p=0.027) and reduced index of myocardium (IM) (9.9+/-7.4 vs. 18.0+/-6.3; p=0.041) were found in patients with a compensated DM II. Patients with a poorly compensated DM II showed a decreased IM (10.9+/-8.6 vs. 16.9+/-5.2; p=0.001) and also a reduced IRSA (4.1+/-3.5 vs. 6.3+/-1.9; p=0.001). The results proved favorable changes in ANS cardiovascular control of patients with DM II after a spa treatment, especially in terms of reducing the sympathoadrenal system activity

  20. The development of selected cardiovascular parameters in patients with type 2 diabetes mellitus during a spa treatment.

    PubMed

    Fialová, E; Kittnar, O

    2015-01-01

    Diabetes mellitus is not just a simple metabolic disorder, however, it is considered to be a cardiovascular disease of a metabolic origin. This is apparent especially when speaking about type 2 diabetes (DM II). The objective of our study was to determine whether a comprehensive spa treatment (procedures and drinking cure) may affect the level of the sympathetic tone of patients suffering from DM II. As an indicator of the sympathetic tone, selected electrocardiographic parameters derived from the heart rate variability and microwave alternans were chosen. There were 96 patients enrolled in our study: 38 patients with poorly controlled DM II and two control groups: 9 patients with compensated DM II and 49 patients, average age without diabetes or other disorders of the glucose metabolism. All received an identical spa treatment and continued their medical therapy. The electrophysiological examination of patients was performed before and after a three-week spa treatment using the KARDiVAR system. Parameters derived from the analysis of heart rate variability (HRV), microvolt T-wave alternans, and microvolt R-wave alternans were analyzed in order to evaluate the tones of the autonomic nervous system (ANS). The control group showed a slight increase of parameter the index of activity of regulatory systems (IRSA) (4.4+/-1.3 vs. 3.8+/-1.4; p=0.006) after the spa treatment, while increased heart rate (80.9+/-11.0 vs. 74.6+/-9.6; p=0.028), reduced index of centralization (IC) (1.3+/-0.6 vs. 2.9+/-1.4; p=0.027) and reduced index of myocardium (IM) (9.9+/-7.4 vs. 18.0+/-6.3; p=0.041) were found in patients with a compensated DM II. Patients with a poorly compensated DM II showed a decreased IM (10.9+/-8.6 vs. 16.9+/-5.2; p=0.001) and also a reduced IRSA (4.1+/-3.5 vs. 6.3+/-1.9; p=0.001). The results proved favorable changes in ANS cardiovascular control of patients with DM II after a spa treatment, especially in terms of reducing the sympathoadrenal system activity

  1. Natural Anti-Infective Pulmonary Proteins: In Vivo Cooperative Action of Surfactant Protein SP-A and the Lung Antimicrobial Peptide SP-BN.

    PubMed

    Coya, Juan Manuel; Akinbi, Henry T; Sáenz, Alejandra; Yang, Li; Weaver, Timothy E; Casals, Cristina

    2015-08-15

    The anionic antimicrobial peptide SP-B(N), derived from the N-terminal saposin-like domain of the surfactant protein (SP)-B proprotein, and SP-A are lung anti-infective proteins. SP-A-deficient mice are more susceptible than wild-type mice to lung infections, and bacterial killing is enhanced in transgenic mice overexpressing SP-B(N). Despite their potential anti-infective action, in vitro studies indicate that several microorganisms are resistant to SP-A and SP-B(N). In this study, we test the hypothesis that these proteins act synergistically or cooperatively to strengthen each other's microbicidal activity. The results indicate that the proteins acted synergistically in vitro against SP-A- and SP-B(N)-resistant capsulated Klebsiella pneumoniae (serotype K2) at neutral pH. SP-A and SP-B(N) were able to interact in solution (Kd = 0.4 μM), which enabled their binding to bacteria with which SP-A or SP-B(N) alone could not interact. In vivo, we found that treatment of K. pneumoniae-infected mice with SP-A and SP-B(N) conferred more protection against K. pneumoniae infection than each protein individually. SP-A/SP-B(N)-treated infected mice showed significant reduction of bacterial burden, enhanced neutrophil recruitment, and ameliorated lung histopathology with respect to untreated infected mice. In addition, the concentrations of inflammatory mediators in lung homogenates increased early in infection in contrast with the weak inflammatory response of untreated K. pneumoniae-infected mice. Finally, we found that therapeutic treatment with SP-A and SP-B(N) 6 or 24 h after bacterial challenge conferred significant protection against K. pneumoniae infection. These studies show novel anti-infective pathways that could drive development of new strategies against pulmonary infections.

  2. Genomic and Functional Characterization of the Unusual pLOCK 0919 Plasmid Harboring the spaCBA Pili Cluster in Lactobacillus casei LOCK 0919

    PubMed Central

    Aleksandrzak-Piekarczyk, Tamara; Koryszewska-Bagińska, Anna; Grynberg, Marcin; Nowak, Adriana; Cukrowska, Bożena; Kozakova, Hana; Bardowski, Jacek

    2016-01-01

    Here, we report the extensive bioinformatic and functional analyses of the unusual pLOCK 0919, a plasmid originating from the probiotic Lactobacillus casei LOCK 0919 strain. This plasmid is atypical because it harbors the spaCBA-srtC gene cluster encoding SpaCBA pili. We show that all other spaCBA-srtC sequences of the Lactobacillus genus that have been previously described and deposited in GenBank are present in the chromosomal DNA. Another important observation for pLOCK 0919 is that the spaCBA-srtC gene cluster and its surrounding genes are highly similar to the respective DNA region that is present in the most well-known and active SpaCBA pili producer, the probiotic Lactobacillus rhamnosus GG strain. Our results demonstrate that the spaCBA-srtC clusters of pLOCK 0919 and L. rhamnosus GG are genealogically similar, located in DNA regions that are rich in transposase genes and are poorly conserved among the publicly available sequences of Lactobacillus sp. In contrast to chromosomally localized pilus gene clusters from L. casei and Lactobacillus paracasei, the plasmidic spaC of L. casei LOCK 0919 is expressed and undergoes a slight glucose-induced repression. Moreover, results of series of in vitro tests demonstrate that L. casei LOCK 0919 has an adhesion potential, which is largely determined by the presence of the pLOCK 0919 plasmid. In particular, the plasmid occurrence positively influenced the hydrophobicity and aggregation abilities of L. casei LOCK 0919. Moreover, in vivo studies indicate that among the three Lactobacillus strains used to colonize the gastrointestinal tract of germ-free mice, already after 2 days of colonization, L. casei LOCK 0919 became the dominant strain and persisted there for at least 48 days. PMID:26637469

  3. Student Project in Anatomy (SPA) – Making the First Year Medical Students Responsible and Creative

    PubMed Central

    Mishra, Snigdha; George, Bincy M; Kumar, Naveen

    2016-01-01

    Introduction Creativity is a combination of ones’ capacity to think outside the box, the gained knowledge and the passion for creating something. It very easily and effectively provides the creator a chance to be responsible for his/her creation and acts as a confidence booster for him/her. Creativity is inherent, but needs to be polished and nurtured. If nurtured well through proper motivation, the creator excels leaps and bounds. It develops an empathetic behaviour in the creator, when he allows his creations to be used by others. Aim The study was done to generate learning resources through academically good students and make them available for the entire class. Materials and Methods Academically, top 16 students were involved in a project of their choice. The projects included preparation of question answers, powerpoint presentations, cross-word puzzles, videos, models, atlases and wall hangers etc., ten weeks were given to finish the project. The project was guided and monitored by teachers. The end product of the project was given to the entire class for use. The perception of users of the end products of the projects was recorded through mini interviews. Results All the students who took part in the project liked working on the project. They felt motivated, rewarded and had mastery on the topic which they used in the project. The students who did not do the project but used the end product of the project also liked the project work. They felt that the end products of the projects were simple, informative and creative. Conclusion By participating in Student Project in Anatomy (SPA), the students get to show their total potential through these creative ways. It provides a fresh and welcome change from the common routine followed otherwise in medical schools. The outcome of the projects can help the entire class. This type of projects can be easily tailored into existing curriculum and in disciplines other than anatomy too. PMID:27790469

  4. A New Search for Lunar Mascon Basins using Detrended Kaguya (SELENE) Gravity: Implications for GRAIL

    NASA Astrophysics Data System (ADS)

    Dombard, A. J.; Hauck, S. A.; Balcerski, J.

    2012-12-01

    The collection of GRAIL data and imminent release of its first gravity models will revolutionize understanding of the lunar interior, which motivates an assessment of the current state of knowledge. A primary goal of the GRAIL mission is to understand better mascon basins, large impact craters that display significant positive free-air and Bouguer gravity anomalies. Discovered in a handful of nearside basins during preparations for the Apollo landings and recently expanded by the global Kaguya (SELENE) gravity models, an important question is why is not every large crater a mascon basin, as less than half of the 41 impact basins > 300 km in diameter (minus South Pole-Aitken) have been previously determined to be mascons. An issue not generally considered in the identification of mascons is that the topography, and hence Bouguer gravity, display long-wavelength regional signals that might mask some mascons. Here, we use the SGM100i Kaguya gravity model and LRO's LOLA shape model to examine the free-air, topographic (arising solely from topography), and Bouguer gravity, detrended by omitting the first 5 spherical harmonic degrees from our expansions. In contrast to past studies, we find that most large basins (28 of 41) display characteristics of mascons (e.g., a strong positive Bouguer anomaly generally narrower than the surface rim). Negative annuli surrounding the central highs in the free-air gravity do not exist in the Bouguer gravity, with only 2 definitive exceptions. The fact that the majority of the Bouguer anomalies are narrower than the basin rim and that the negative free-air annulus appears to be a product of the surface topography has implications for the formation of the basins. We propose that beneath a forming large basin, the mantle uplifts in response to the large isostatic imbalance with the transient crater, while the surface topography forms from not only upward but inward collapse of the transient crater's rim wall and adjustment of the melt

  5. The Mxi-Spa Type III Secretory Pathway of Shigella flexneri Requires an Outer Membrane Lipoprotein, MxiM, for Invasin Translocation

    PubMed Central

    Schuch, Raymond; Maurelli, Anthony T.

    1999-01-01

    Invasion of epithelial cells by Shigella flexneri is mediated by a set of translocated bacterial invasins, the Ipa proteins, and its dedicated type III secretion system, called Mxi-Spa. We show here that mxiM, part of the mxi-spa locus in the S. flexneri virulence plasmid, encodes an indispensable type III secretion apparatus component, required for both Ipa translocation and tissue culture cell invasion. We demonstrated that mature MxiM, first identified as a putative lipoprotein, is lipidated in vivo. Consistent with features of known lipoproteins, MxiM (i) can be labeled with [3H]palmitate and [2-3H]glycerol, (ii) is associated with the cell envelope, (iii) is secreted independently of the type III pathway, and (iv) requires an intact lipoprotein modification and processing site for full activity. The lipidated form of MxiM was detected primarily in the outer membrane, where it establishes a peripheral association with the inner leaflet. Through analysis of subcellular Ipa distribution in a mxiM null mutant background, MxiM was found to be required for the assembly and/or function of outer, but not inner, membrane regions of Mxi-Spa. This function probably requires interactions with other Mxi-Spa subunits within the periplasmic space. We discuss implications of these findings with respect to the function of MxiM and the structure of Mxi-Spa as a whole. PMID:10085046

  6. Lactobacillus rhamnosus GG and its SpaC pilus adhesin modulate inflammatory responsiveness and TLR-related gene expression in the fetal human gut

    PubMed Central

    Ganguli, Kriston; Collado, Maria Carmen; Rautava, Jaana; Lu, Lei; Satokari, Reetta; von Ossowski, Ingemar; Reunanen, Justus; de Vos, Willem M.; Palva, Airi; Isolauri, Erika; Salminen, Seppo; Walker, W. Allan; Rautava, Samuli

    2015-01-01

    Background Bacterial contact in utero modulates fetal and neonatal immune responses. Maternal probiotic supplementation reduces the risk of immune-mediated disease in the infant. We investigated the immunomodulatory properties of live Lactobacillus rhamnosus GG and its SpaC pilus adhesin in human fetal intestinal models. Methods TNF-α mRNA expression was measured by qPCR in a human fetal intestinal organ culture model exposed to live L. rhamnosus GG and proinflammatory stimuli. Binding of recombinant SpaC pilus protein to intestinal epithelial cells was assessed in human fetal intestinal organ culture and the human fetal intestinal epithelial cell line H4 by immunohistochemistry and immunofluorescence, respectively. TLR-related gene expression in fetal ileal organ culture after exposure to recombinant SpaC was assessed by qPCR. Results Live L. rhamnosus GG significantly attenuates pathogen-induced TNF-α mRNA expression in the human fetal gut. Recombinant SpaC protein was found to adhere to the fetal gut and to modulate varying levels of TLR-related gene expression. Conclusion The human fetal gut is responsive to luminal microbes. L. rhamnosus GG significantly attenuates fetal intestinal inflammatory responses to pathogenic bacteria. The L. rhamnosus GG pilus adhesin SpaC binds to immature human intestinal epithelial cells and directly modulates intestinal epithelial cell innate immune gene expression. PMID:25580735

  7. Reserves in Western Basins

    SciTech Connect

    Caldwell, R.H.; Cotton, B.W.

    1993-12-31

    The objective of this project is to investigate the reserves potential of overpressured tight (OPT) gas reservoirs in three Rocky Mountain basins. These are the Greater Green River Basin (GGRB), Uinta Basin and Piceance Basin. By documenting productive characteristics in these basins and characterizing the nature of the vast gas resources in place, the reserves potential may be understood and quantified. Through this understanding, it is hoped that the oil and gas industry will be encouraged to pursue exploitation of this resource. At this point in time, the GGRB work has been completed and the final report submitted for publication. Work on the Uinta basin has just commenced and work on the Piceance basin will commence next year. Since the GGRB portion of this project has been completed, further discussion centers upon this Basin.

  8. Spa Typing of Staphylococcus aureus Strains Isolated From Clinical Specimens of Patients With Nosocomial Infections in Tehran, Iran

    PubMed Central

    Goudarzi, Mehdi; Fazeli, Maryam; Goudarzi, Hossein; Azad, Mehdi; Seyedjavadi, Sima Sadat

    2016-01-01

    Background The incidence of nosocomial Staphylococcus aureus infection is increasing annually and becoming a true global challenge. The pattern of Staphylococcus aureus protein A (spa) types in different geographic regions is diverse. Objectives This study determined the prevalence of methicillin-resistant S. aureus and different spa types in S. aureus clinical isolates. Materials and Methods During a six-month period, 90 S. aureus isolates were recovered from 320 clinical specimens. The in vitro susceptibility of various S. aureus isolates to 16 antibiotic discs was assessed using the Kirby-Bauer disk diffusion method. Molecular typing was carried out with S. aureus protein A typing via polymerase chain reaction. Results The frequency of methicillin-resistant S. aureus in our study was 88.9%. Twenty-three (25.5%) isolates were positive for panton-valentine leukocidin encoding genes. S. aureus presented a high resistance rate to ampicillin (100%) and penicillin (100%). No resistance was observed to vancomycin, teicoplanin, or linezolid. The rates of resistance to the majority of antibiotics tested varied between 23.3% and 82.2%. The rate of multidrug resistance among these clinical isolates was 93.3%. The 90 S. aureus isolates were classified into five S. aureus protein A types: t037 (33.3%), t030 (22.2%), t790 (16.7%), t969 (11.1%), and t044 (7.7%). Eight (8.9%) isolates were not typable using the S. aureus protein A typing method. Conclusions We report a high methicillin-resistant S. aureus rate in our hospital. Additionally, t030 and t037 were the predominant spa-types among hospital-associated S. aureus. Our findings emphasize the need for continuous surveillance to prevent the dissemination of multidrug resistance among different S. aureus protein A types in Iran. PMID:27679706

  9. [Selection of Characteristic Wavelengths Using SPA and Qualitative Discrimination of Mildew Degree of Corn Kernels Based on SVM].

    PubMed

    Yuan, Ying; Wang, Wei; Chu, Xuan; Xi, Ming-jie

    2016-01-01

    The feasibility of Fourier transform near infrared (FT-NIR) spectroscopy with spectral range between 833 and 2 500 nm to detect the moldy corn kernels with different levels of mildew was verified in this paper. Firstly, to avoid the influence of noise, moving average smoothing was used for spectral data preprocessing after four common pretreatment methods were compared. Then to improve the prediction performance of the model, SPXY (sample set partitioning based on joint x-y distance) was selected and used for sample set partition. Furthermore, in order to reduce the dimensions of the original spectral data, successive projection algorithm (SPA) was adopted and ultimately 7 characteristic wavelengths were extracted, the characteristic wave-lengths were 833, 927, 1 208, 1 337, 1 454, 1 861, 2 280 nm. The experimental results showed when the spectrum data of the 7 characteristic wavelengths were taken as the input of SVM, the radial basic function (RBF) used as the kernel function, and kernel parameter C = 7 760 469, γ = 0.017 003, the classification accuracies of the established SVM model were 97.78% and 93.33% for the training and testing sets respectively. In addition, the independent validation set was selected in the same standard, and used to verify the model. At last, the classification accuracy of 91.11% for the independent validation set was achieved. The result indicated that it is feasible to identify and classify different degree of moldy corn grain kernels using SPA and SVM, and characteristic wavelengths selected by SPA in this paper also lay a foundation for the online NIR detection of mildew corn kernels. PMID:27228772

  10. Spa Typing of Staphylococcus aureus Strains Isolated From Clinical Specimens of Patients With Nosocomial Infections in Tehran, Iran

    PubMed Central

    Goudarzi, Mehdi; Fazeli, Maryam; Goudarzi, Hossein; Azad, Mehdi; Seyedjavadi, Sima Sadat

    2016-01-01

    Background The incidence of nosocomial Staphylococcus aureus infection is increasing annually and becoming a true global challenge. The pattern of Staphylococcus aureus protein A (spa) types in different geographic regions is diverse. Objectives This study determined the prevalence of methicillin-resistant S. aureus and different spa types in S. aureus clinical isolates. Materials and Methods During a six-month period, 90 S. aureus isolates were recovered from 320 clinical specimens. The in vitro susceptibility of various S. aureus isolates to 16 antibiotic discs was assessed using the Kirby-Bauer disk diffusion method. Molecular typing was carried out with S. aureus protein A typing via polymerase chain reaction. Results The frequency of methicillin-resistant S. aureus in our study was 88.9%. Twenty-three (25.5%) isolates were positive for panton-valentine leukocidin encoding genes. S. aureus presented a high resistance rate to ampicillin (100%) and penicillin (100%). No resistance was observed to vancomycin, teicoplanin, or linezolid. The rates of resistance to the majority of antibiotics tested varied between 23.3% and 82.2%. The rate of multidrug resistance among these clinical isolates was 93.3%. The 90 S. aureus isolates were classified into five S. aureus protein A types: t037 (33.3%), t030 (22.2%), t790 (16.7%), t969 (11.1%), and t044 (7.7%). Eight (8.9%) isolates were not typable using the S. aureus protein A typing method. Conclusions We report a high methicillin-resistant S. aureus rate in our hospital. Additionally, t030 and t037 were the predominant spa-types among hospital-associated S. aureus. Our findings emphasize the need for continuous surveillance to prevent the dissemination of multidrug resistance among different S. aureus protein A types in Iran.

  11. [Selection of Characteristic Wavelengths Using SPA and Qualitative Discrimination of Mildew Degree of Corn Kernels Based on SVM].

    PubMed

    Yuan, Ying; Wang, Wei; Chu, Xuan; Xi, Ming-jie

    2016-01-01

    The feasibility of Fourier transform near infrared (FT-NIR) spectroscopy with spectral range between 833 and 2 500 nm to detect the moldy corn kernels with different levels of mildew was verified in this paper. Firstly, to avoid the influence of noise, moving average smoothing was used for spectral data preprocessing after four common pretreatment methods were compared. Then to improve the prediction performance of the model, SPXY (sample set partitioning based on joint x-y distance) was selected and used for sample set partition. Furthermore, in order to reduce the dimensions of the original spectral data, successive projection algorithm (SPA) was adopted and ultimately 7 characteristic wavelengths were extracted, the characteristic wave-lengths were 833, 927, 1 208, 1 337, 1 454, 1 861, 2 280 nm. The experimental results showed when the spectrum data of the 7 characteristic wavelengths were taken as the input of SVM, the radial basic function (RBF) used as the kernel function, and kernel parameter C = 7 760 469, γ = 0.017 003, the classification accuracies of the established SVM model were 97.78% and 93.33% for the training and testing sets respectively. In addition, the independent validation set was selected in the same standard, and used to verify the model. At last, the classification accuracy of 91.11% for the independent validation set was achieved. The result indicated that it is feasible to identify and classify different degree of moldy corn grain kernels using SPA and SVM, and characteristic wavelengths selected by SPA in this paper also lay a foundation for the online NIR detection of mildew corn kernels.

  12. Continental pollution in the Western Mediterranean Basin: vertical profiles of aerosol and trace gases measured over the sea during TRAQA 2012 and SAFMED 2013

    NASA Astrophysics Data System (ADS)

    Di Biagio, C.; Doppler, L.; Gaimoz, C.; Grand, N.; Ancellet, G.; Raut, J.-C.; Beekmann, M.; Borbon, A.; Sartelet, K.; Attié, J.-L.; Ravetta, F.; Formenti, P.

    2015-03-01

    In this study we present airborne observations of aerosol and trace gases obtained over the sea in the Western Mediterranean Basin during the TRAQA (TRansport and Air QuAlity) and SAFMED (Secondary Aerosol Formation in the MEDiterranean) campaigns in summers 2012 and 2013. A total of 23 vertical profiles were measured up to 5000 m a.s.l. over an extended area (40-45° N latitude and 2° W-12° E longitude) including the Gulf of Genoa, Southern France, the Gulf of Lion, and the Spanish coast. TRAQA and SAFMED successfully measured a wide range of meteorological conditions which favoured the pollution export from different sources located around the basin. Also, several events of dust outflows were measured during the campaigns. Observations from the present study indicate that continental pollution largely affects the Western Mediterranean both close to coastal regions and in the open sea as far as ~250 km from the coastline. Aerosol layers not specifically linked with Saharan dust outflows are distributed ubiquitously which indicates quite elevated levels of background pollution throughout the Western Basin. The measured aerosol scattering coefficient varies between ~20 and 120 M m-1, while carbon monoxide (CO) and ozone (O3) mixing ratios are in the range of 60-170 and 30-85 ppbv, respectively. Pollution reaches 3000-4000 m in altitude and presents a very complex and highly stratified structure characterized by fresh and aged layers both in the boundary layer and in the free troposphere. Within pollution plumes the measured particle concentration in the Aitken (0.004-0.1 μm) and accumulation (0.1-1.0 μm) modes is between ˜ 100 and 5000-6000 s cm-3 (standard cm-3), which is comparable to the aerosol concentration measured in continental urban areas. Additionally, our measurements indicate the presence of highly concentrated Aitken layers (10 000-15 000 s cm-3) observed both close to the surface and in the free troposphere, possibly linked to the influence of new

  13. Characterization of Staphylococcus aureus from distinct geographic locations in China: an increasing prevalence of spa-t030 and SCCmec type III.

    PubMed

    Chen, Yong; Liu, Zhengxiang; Duo, Libo; Xiong, Jie; Gong, Yanwen; Yang, Jiyong; Wang, Zhanke; Wu, Xuqin; Lu, Zhongyi; Meng, Xiangzhao; Zhao, Jingya; Zhang, Changjian; Wang, Fang; Zhang, Yulong; Zhang, Mengqiang; Han, Li

    2014-01-01

    Staphylococcus aureus belongs to one of the most common bacteria causing healthcare and community associated infections in China, but their molecular characterization has not been well studied. From May 2011 to June 2012, a total of 322 non-duplicate S. aureus isolates were consecutively collected from seven tertiary care hospitals in seven cities with distinct geographical locations in China, including 171 methicillin sensitive S. aureus (MSSA) and 151 MRSA isolates. All isolates were characterized by spa typing. The presence of virulence genes was tested by PCR. MRSA were further characterized by SCCmec typing. Seventy four and 16 spa types were identified among 168 MSSA and 150 MRSA, respectively. One spa type t030 accounted for 80.1% of all MRSA isolates, which was higher than previously reported, while spa-t037 accounted for only 4.0% of all MRSA isolates. The first six spa types (t309, t189, t034, t377, t078 and t091) accounted for about one third of all MSSA isolates. 121 of 151 MRSA isolates (80.1%) were identified as SCCmec type III. pvl gene was found in 32 MSSA (18.7%) and 5 MRSA (3.3%) isolates, with ST22-MSSA-t309 as the most commonly identified strain. Compared with non-epidemic MRSA clones, epidemic MRSA clones (corresponding to ST239) exhibited a lower susceptibility to rifampin, ciprofloxacin, gentamicin and trimethoprim-sulfamethoxazole, a higher prevalence of sea gene and a lower prevalence of seb, sec, seg, sei and tst genes. The increasing prevalence of multidrug resistant spa-t030 MRSA represents a major public health problem in China.

  14. Smooth pursuit adaptation (SPA) exhibits features useful to compensate changes in the properties of the smooth pursuit eye movement system due to usage.

    PubMed

    Dash, Suryadeep; Thier, Peter

    2013-01-01

    Smooth-pursuit adaptation (SPA) refers to the fact that pursuit gain in the early, still open-loop response phase of the pursuit eye movement can be adjusted based on experience. For instance, if the target moves initially at a constant velocity for ~100-200 ms and then steps to a higher velocity, subjects learn to up-regulate the pursuit gain associated with the initial target velocity (gain-increase SPA) in order to reduce the retinal error resulting from the velocity step. Correspondingly, a step to a lower target velocity leads to a decrease in gain (gain-decrease SPA). In this study we demonstrate that the increase in peak eye velocity during gain-increase SPA is a consequence of expanding the duration of the eye acceleration profile while the decrease in peak velocity during gain-decrease SPA results from reduced peak eye acceleration but unaltered duration. Furthermore, we show that carrying out stereotypical smooth pursuit eye movements elicited by constant velocity target ramps for several hundred trials (=test of pursuit resilience) leads to a clear drop in initial peak acceleration, a reflection of oculomotor and/or cognitive fatigue. However, this drop in acceleration gets compensated by an increase in the duration of the acceleration profile, thereby keeping initial pursuit gain constant. The compensatory expansion of the acceleration profile in the pursuit resilience experiment is reminiscent of the one leading to gain-increase SPA, suggesting that both processes tap one and the same neuronal mechanism warranting a precise acceleration-duration trade-off. Finally, we show that the ability to adjust acceleration duration during pursuit resilience depends on the integrity of the oculomotor vermis (OMV) as indicated by the complete loss of the duration adjustment following a surgical lesion of the OMV in one rhesus monkey we could study.

  15. SpA, ClfA, and FnbA Genetic Variations Lead to Staphaurex Test-Negative Phenotypes in Bovine Mastitis Staphylococcus aureus Isolates▿

    PubMed Central

    Stutz, Katrin; Stephan, Roger; Tasara, Taurai

    2011-01-01

    Staphylococcus aureus encodes many proteins that act as virulence factors, leading to a variety of diseases, including mastitis in cows. Among these virulence factors, SpA, ClfA, ClfB, FnbA, and FnbB are important for the ability of S. aureus to adhere to and invade host cells as well as to evade host immune responses. The interaction between these S. aureus surface proteins and human immunoglobulin G and fibrinogen that are coupled to latex particles is utilized to induce latex agglutination reactions, which are used widely in diagnostic kits for confirmation of presumptive S. aureus isolates. In this study, the Staphaurex latex agglutination test was performed on a collection of confirmed bovine mastitis S. aureus isolates. Notably, 54% (43/79 isolates) of these isolates exhibited latex agglutination-negative phenotypes (Staphaurex-negative result). To gain insights into the reasons for the high frequency of Staphaurex-negative bovine mastitis S. aureus isolates, the spa, clfA, clfB, fnbA, and fnbB genes were examined. Specific genetic changes in spa, clfA, and fnbA, as well as a loss of fnbB, which may impair SpA, ClfA, FnbA, and FnbB functions in latex agglutination reactions, were detected in Staphaurex-negative S. aureus isolates. The genetic changes included a premature stop codon in the spa gene, leading to a truncated SpA protein that is unable to participate in S. aureus cell-mediated agglutination of latex particles. In addition, clfA and fnbA genetic polymorphisms were detected that were linked to ClfA and FnbA amino acid changes that may significantly reduce fibrinogen-binding activity. The genetic variations in these S. aureus isolates might also have implications for their bovine mastitis virulence capacity. PMID:21147952

  16. [Near-infrared spectra combining with CARS and SPA algorithms to screen the variables and samples for quantitatively determining the soluble solids content in strawberry].

    PubMed

    Li, Jiang-bo; Guo, Zhi-ming; Huang, Wen-qian; Zhang, Bao-hua; Zhao, Chun-jiang

    2015-02-01

    In using spectroscopy to quantitatively or qualitatively analyze the quality of fruit, how to obtain a simple and effective correction model is very critical for the application and maintenance of the developed model. Strawberry as the research object, this research mainly focused on selecting the key variables and characteristic samples for quantitatively determining the soluble solids content. Competitive adaptive reweighted sampling (CARS) algorithm was firstly proposed to select the spectra variables. Then, Samples of correction set were selected by successive projections algorithm (SPA), and 98 characteristic samples were obtained. Next, based on the selected variables and characteristic samples, the second variable selection was performed by using SPA method. 25 key variables were obtained. In order to verify the performance of the proposed CARS algorithm, variable selection algorithms including Monte Carlo-uninformative variable elimination (MC-UVE) and SPA were used as the comparison algorithms. Results showed that CARS algorithm could eliminate uninformative variables and remove the collinearity information at the same time. Similarly, in order to assess the performance of the proposed SPA algorithm for selecting the characteristic samples, SPA algorithm was compared with classical Kennard-Stone algorithm Results showed that SPA algorithm could be used for selection of the characteristic samples in the calibration set. Finally, PLS and MLR model for quantitatively predicting the SSC (soluble solids content) in the strawberry were proposed based on the variables/samples subset (25/98), respectively. Results show that models built by using the 0.59% and 65.33% information of original variables and samples could obtain better performance than using the ones obtained by using all information of the original variables and samples. MLR model was the best with R(pre)2 = 0.9097, RMSEP=0.3484 and RPD = 3.3278.

  17. Clonal Structure and Characterization of Staphylococcus aureus Strains from Invasive Infections in Paediatric Patients from South Poland: Association between Age, spa Types, Clonal Complexes, and Genetic Markers.

    PubMed

    Ilczyszyn, Weronika M; Sabat, Artur J; Akkerboom, Viktoria; Szkarlat, Anna; Klepacka, Joanna; Sowa-Sierant, Iwona; Wasik, Barbara; Kosecka-Strojek, Maja; Buda, Aneta; Miedzobrodzki, Jacek; Friedrich, Alexander W

    2016-01-01

    The aim of current study was to examine clonal structure and genetic profile of invasive Staphylococcus aureus isolates recovered from infants and children treated at the Jagiellonian University Children's Hospital of Krakow, Poland. The 107 invasive S. aureus isolates, collected between February 2012 and August 2014, were analysed retrospectively. Antimicrobial susceptibility testing, spa typing and DNA microarray analysis were performed to determine clonal distribution, diversity and gene content in regard to patients characteristics. In total, 107 isolates were recovered from 88 patients with clinical symptoms of invasive bacterial infection. The final set of 92 non-duplicate samples included 38 MRSA isolates. Additionally, a set of 54 S. aureus isolates collected during epidemiological screening was genotyped and analysed. There were 72 healthcare-associated (HCA) and 20 community-onset (CO) infection events caused by 33 and 5 MRSA isolates, respectively. The majority of isolates were affiliated with the major European clonal complexes CC5 (t003, spa-CC 002), CC45 (spa-CC 015), CC7 or CC15 (t084, t091, spa-CC 084). Two epidemic clones (CC5-MRSA-II or CC45-MRSA-IV) dominated among MRSA isolates, while MSSA population contained 15 different CCs. The epidemiological screening isolates belonged to similar genetic lineages as those collected from invasive infection cases. The HCA infection events, spa types t003, t2642 or CC5 were significantly associated with infections occurring in neonates and children under 5 years of age. Moreover, carriage of several genetic markers, including erm(A), sea (N315), egc-cluster, chp was significantly higher in isolates obtained from children in this age group. The spa types t091 and t008 were underrepresented among patients aged 5 years or younger, whereas spa type t008, CC8 and presence of splE was associated with infection in children aged 10 years or older. The HCA-MRSA strains were most frequently found in children under 5

  18. Clonal Structure and Characterization of Staphylococcus aureus Strains from Invasive Infections in Paediatric Patients from South Poland: Association between Age, spa Types, Clonal Complexes, and Genetic Markers

    PubMed Central

    Ilczyszyn, Weronika M.; Sabat, Artur J.; Akkerboom, Viktoria; Szkarlat, Anna; Klepacka, Joanna; Sowa-Sierant, Iwona; Wasik, Barbara; Kosecka-Strojek, Maja; Buda, Aneta; Miedzobrodzki, Jacek; Friedrich, Alexander W.

    2016-01-01

    The aim of current study was to examine clonal structure and genetic profile of invasive Staphylococcus aureus isolates recovered from infants and children treated at the Jagiellonian University Children’s Hospital of Krakow, Poland. The 107 invasive S. aureus isolates, collected between February 2012 and August 2014, were analysed retrospectively. Antimicrobial susceptibility testing, spa typing and DNA microarray analysis were performed to determine clonal distribution, diversity and gene content in regard to patients characteristics. In total, 107 isolates were recovered from 88 patients with clinical symptoms of invasive bacterial infection. The final set of 92 non-duplicate samples included 38 MRSA isolates. Additionally, a set of 54 S. aureus isolates collected during epidemiological screening was genotyped and analysed. There were 72 healthcare-associated (HCA) and 20 community-onset (CO) infection events caused by 33 and 5 MRSA isolates, respectively. The majority of isolates were affiliated with the major European clonal complexes CC5 (t003, spa-CC 002), CC45 (spa-CC 015), CC7 or CC15 (t084, t091, spa-CC 084). Two epidemic clones (CC5-MRSA-II or CC45-MRSA-IV) dominated among MRSA isolates, while MSSA population contained 15 different CCs. The epidemiological screening isolates belonged to similar genetic lineages as those collected from invasive infection cases. The HCA infection events, spa types t003, t2642 or CC5 were significantly associated with infections occurring in neonates and children under 5 years of age. Moreover, carriage of several genetic markers, including erm(A), sea (N315), egc-cluster, chp was significantly higher in isolates obtained from children in this age group. The spa types t091 and t008 were underrepresented among patients aged 5 years or younger, whereas spa type t008, CC8 and presence of splE was associated with infection in children aged 10 years or older. The HCA-MRSA strains were most frequently found in children under 5

  19. Clonal Structure and Characterization of Staphylococcus aureus Strains from Invasive Infections in Paediatric Patients from South Poland: Association between Age, spa Types, Clonal Complexes, and Genetic Markers.

    PubMed

    Ilczyszyn, Weronika M; Sabat, Artur J; Akkerboom, Viktoria; Szkarlat, Anna; Klepacka, Joanna; Sowa-Sierant, Iwona; Wasik, Barbara; Kosecka-Strojek, Maja; Buda, Aneta; Miedzobrodzki, Jacek; Friedrich, Alexander W

    2016-01-01

    The aim of current study was to examine clonal structure and genetic profile of invasive Staphylococcus aureus isolates recovered from infants and children treated at the Jagiellonian University Children's Hospital of Krakow, Poland. The 107 invasive S. aureus isolates, collected between February 2012 and August 2014, were analysed retrospectively. Antimicrobial susceptibility testing, spa typing and DNA microarray analysis were performed to determine clonal distribution, diversity and gene content in regard to patients characteristics. In total, 107 isolates were recovered from 88 patients with clinical symptoms of invasive bacterial infection. The final set of 92 non-duplicate samples included 38 MRSA isolates. Additionally, a set of 54 S. aureus isolates collected during epidemiological screening was genotyped and analysed. There were 72 healthcare-associated (HCA) and 20 community-onset (CO) infection events caused by 33 and 5 MRSA isolates, respectively. The majority of isolates were affiliated with the major European clonal complexes CC5 (t003, spa-CC 002), CC45 (spa-CC 015), CC7 or CC15 (t084, t091, spa-CC 084). Two epidemic clones (CC5-MRSA-II or CC45-MRSA-IV) dominated among MRSA isolates, while MSSA population contained 15 different CCs. The epidemiological screening isolates belonged to similar genetic lineages as those collected from invasive infection cases. The HCA infection events, spa types t003, t2642 or CC5 were significantly associated with infections occurring in neonates and children under 5 years of age. Moreover, carriage of several genetic markers, including erm(A), sea (N315), egc-cluster, chp was significantly higher in isolates obtained from children in this age group. The spa types t091 and t008 were underrepresented among patients aged 5 years or younger, whereas spa type t008, CC8 and presence of splE was associated with infection in children aged 10 years or older. The HCA-MRSA strains were most frequently found in children under 5

  20. Different growth rates in amoeba of genotypically related environmental and clinical Legionella pneumophila strains isolated from a thermal spa.

    PubMed Central

    Molmeret, M.; Jarraud, S.; Mori, J. P.; Pernin, P.; Forey, F.; Reyrolle, M.; Vandenesch, F.; Etienne, J.; Farge, P.

    2001-01-01

    Two cases of legionellosis occurring 3 years apart were acquired in the same French thermal spa and were apparently due to the same strain of Legionella pneumophila serogroup 1, as shown by genomic macrorestriction analysis. Minor differences between the two isolates were found by random amplification PCR profiling which showed an additional band with one of the isolates. Analysis of 107 L. pneumophila strains isolated from the spa waters by genome macrorestriction failed to identify the infective strain, but a closely related L. pneumophila serogroup 3 strain differing from the clinical isolates by only one band was found. To determine if the clinical L. pneumophila serogroup 1 isolates was better adapted for intracellular multiplication than related serogroup 3 environmental isolates, the growth kinetics of six isolates were determined in co-culture with Acanthamoeba lenticulata. One clinical isolate failed to grow within amoeba, while the other clinical isolate yielded the highest increase in bacterial cell count per amoeba (1,200%) and the environmental isolates gave intermediate values. Genetic analysis of L. pneumophila isolates by DNA macrorestriction does not therefore appear to reflect their growth kinetics within amoeba, and is not sufficiently discriminatory to identify potentially virulent strains. PMID:11349974

  1. Pollen grain morphology of Fabaceae in the Special Protection Area (SPA) Pau-de-Fruta, Diamantina, Minas Gerais, Brazil.

    PubMed

    Luz, Cynthia F P da; Maki, Erica S; Horák-Terra, Ingrid; Vidal-Torrado, Pablo; Mendonça Filho, Carlos Victor

    2013-01-01

    The presented paper considered the pollen morphology of thirteen species belonging to seven genera of the Fabaceae family occurring in the Pau-de-Fruta Special Protection Area (SPA), Diamantina, state of Minas Gerais, Brazil. The pollen grains of six species of Chamaecrista [C. cathartica (Mart.) H.S. Irwin & Barneby, C. debilis Vogel, C. flexuosa (L.) Greene, C. hedysaroides (Vogel) H.S. Irwin & Barneby, C. glandulosa (L.) Greene, and C. papillata H.S. Irwin & Barneby] have a similar morphology, characterized by three long colporated apertures with a central constriction. The species share specific morphological features regarding pollen size, endoaperture type (circular, lalongate or lolongate) and SEM ornamentation patterns of the exine (rugulate with perforations or perforate). Andira fraxinifolia Benth., Dalbergia miscolobium Benth, Galactia martii DC, Periandra mediterranea (Vell.) Taub., Senna rugosa (G.Don) H.S. Irwin & Barneby and Zornia diphylla (L.) Pers showed different pollen types in small to large size; oblate spheroidal to prolate form; colpus or colporus apertures; circular, lalongate or lolongate endoapertures and distinctive SEM ornamentation patterns of the exine (perforate, microreticulate, reticulate or rugulate with perforations). Only Stryphnodendron adstringens (Mart.) Coville presents polyads. The pollen morphology variation of these species allowed the Fabaceae family to be characterized as eurypalynous in the SPA Pau-de-Fruta.

  2. An outbreak of pneumonia and meningitis caused by a previously undescribed gram-negative bacterium in a hot spring spa.

    PubMed Central

    Hubert, B.; de Mahenge, A.; Grimont, F.; Richard, C.; Peloux, Y.; de Mahenge, C.; Fleurette, J.; Grimont, P. A.

    1991-01-01

    An outbreak of infection caused by a previously undescribed Gram-negative bacterium affected people attending a hot (37 degrees C) spring spa in France in 1987. Thirty-five case of pneumonia and two cases of meningitis occurred. None of these patients died. Attack rates were significantly higher for patients above 70 years old and for male patients. An epidemiological comparison of the 26 hospitalized cases with 52 matched controls suggests that spa treatment early on the first day (OR = 4.8) and attendance at the vapour baths (OR = 10.7) were significant risk factors for acquiring the infection. Person-to-person spread was not thought to have occurred. The same bacterium was isolated from the hot spring water. All strains studied shows a single rRNA gene restriction pattern. Epidemiological data indicated that the thermal water was the source of infection. This outbreak stresses the need for increased surveillance of infections in people attending hot spring spas. PMID:1936159

  3. Wine Valley Inn: A mineral water spa in Calistoga, California. Geothermal-energy-system conceptual design and economic feasibility

    SciTech Connect

    Not Available

    1981-10-26

    The purpose of this study is to determine the engineering and economic feasibility for utilizing geothermal energy for air conditioning and service water heating at the Wine Valley Inn, a mineral water spa in Calistoga, California. The study evaluates heating, ventilating, air conditioning and water heating systems suitable for direct heat geothermal application. Due to the excellent geothermal temperatures available at this site, the mechanics and economics of a geothermally powered chilled water cooling system are evaluated. The Wine Valley Inn has the resource potential to have one of the few totally geothermal powered air conditioning and water heating systems in the world. This total concept is completely developed. A water plan was prepared to determine the quantity of water required for fresh water well development based on the special requirements of the project. An economic evaluation of the system is included to justify the added capital investment needed to build the geothermally powered mineral spa. Energy payback calculations are presented. A thermal cascade system is proposed to direct the geothermal water through the energy system to first power the chiller, then the space heating system, domestic hot water, the two spas and finally to heat the swimming pool. The Energy Management strategy required to automatically control this cascade process using industrial quality micro-processor equipment is described. Energy Management controls are selected to keep equipment sizing at a minimum, pump only the amount of geothermal water needed and be self balancing.

  4. A combined STM and SPA-LEED study of the "explosive" nucleation and collective diffusion in Pb/Si(111)

    NASA Astrophysics Data System (ADS)

    Hattab, H.; Hupalo, M.; Hershberger, M. T.; Horn von Hoegen, M.; Tringides, M. C.

    2016-04-01

    A novel type of very fast nucleation was recently found in Pb/Si(111) with 4- to 7-layer high islands becoming crystalline in an "explosive" way, when the Pb deposited amount in the wetting layer is compressed to θc ~ 1.22 ML, well above the metallic Pb(111) density. This "explosive" nucleation is very different from classical nucleation when island growth is more gradual and islands grow in size by single adatom aggregation [8]. In order to identify the key parameters that control the nucleation we used scanning tunneling microscopy (STM) and spot profile analysis low energy electron diffraction (SPA-LEED). It was found that the number and duration of steps in iterative deposition used to approach θc and the flux rate have dramatic effects on the crystallization process. Larger depositions over shorter times induce greater spatial coverage fluctuations, so local areas can reach the critical coverage θc easier. This can trigger the collective motion of the wetting layer from far away to build the Pb islands "explosively". The SPA-LEED experiments show that even low flux experiments in iterative deposition experiments can trigger transfer of material to the superstable 7-layer islands, as seen from the stronger satellite rings close to the (00) spot.

  5. [Measurement of Nitrogen Content in Lettuce Canopy Using Spectroscopy Combined with BiPLS-GA-SPA and ELM].

    PubMed

    Gao, Hong-yan; Mao, Han-ping; Zhang, Xiao-dong

    2016-02-01

    Nitrogen fertilizer is necessary to improve yield and quality of lettuce. Spectroscopy is one of the most effective techniques used to detect crop nitrogen content. In this study, canopy reflectance spectra were acquired under five levels of nitrogen, and then were Savitzky-Golay smoothed, the first-order derivative spectra were calculated from the smoothed spectra to eliminate noise effects. Backward interval partial least squares (BiPLS), genetic algorithm (GA) and successive projections algorithm (SPA) were combined to select the efficient wavelengths. The number of variables was decreased from 2,151 to 8. The optimal intervals or variables were used to build multivariable linear regression (MLR) model, radial basis function neural network (RBFNN) models and extreme learning machine (ELM) models. This work proved that the results of BiPLS-GA-SPA-ELM model was superior to others with RMSEC was 0.241 6%, Rc was 0.934 6, RMSEP was 0.284 2% and Rp was 0.921 8. Our research results may provide a foundation for nutrition regulation and developing instrument. PMID:27209756

  6. Sex work and its associations with alcohol and methamphetamine use among female bar and spa workers in the Philippines.

    PubMed

    Urada, Lianne A; Strathdee, Steffanie A; Morisky, Donald E; Schilling, Robert F; Simbulan, Nymia P; Estacio, Leonardo R; Raj, Anita

    2014-03-01

    To assess the prevalence of sex work and its associations with substance use among female bar/spa workers in the Philippines (N = 498), workers from 54 bar or spa venues in Metro Manila (2009-2010) were surveyed on demographics, drug/alcohol use, abuse history, and sex work. Their median age was 23 years and 35% engaged in sex work. Sex work was independently associated with methamphetamine use (19% vs 4%; adjusted odds ratio [AOR] =2.9, 95% confidence interval [CI] = 1.3-6.2), alcohol use with patrons (49% vs. 27%; AOR = 1.9, 95% CI = 1.1-3.4), and alcohol intoxication during sex (50% vs. 24%; AOR = 2.0, 95% CI = 1.2-3.5), but inversely associated with daily alcohol use (13% vs. 16%; AOR = 0.2, 95% CI = 0.1-0.5). Additional significant covariates included sexual abuse history, younger age, and not having a higher education. Findings suggest that interventions with sex workers in bars and spas should focus on methamphetamine use, alcohol use contexts, and violence victimization, to better meet the needs of this population.

  7. Integration and Comparison of Clementine and Lunar Prospector Data: Global Scale Multielement Analysis1 (Fe, Ti, and Th) of the Lunar Surface

    NASA Astrophysics Data System (ADS)

    Chevrel, S. D.; Pinet, P. C.; Daydou, Y.; Feldman, W. C.

    2002-11-01

    In this paper, we present (1) a statistical analysis, based on a systematic clustering method, of a dataset integrating the global abundance maps of the three elements iron, titanium, and thorium derived from Clementine and Lunar Prospector and (2) a comparison of iron abundances between Clementine and Lunar Prospector. Homogeneous geologic units are compositionally characterized and spatially defined in relation to the major rock types sampled on the Moon. With the lowest abundances of Fe, Ti, and Th found on the Moon, the lunar highland terrains are quite homogeneous with two major large feldspathic units, one being slightly more mafic than the other. Two distinct regions with unique compositions are unambiguously identified: the Procellarum KREEP Terrane (PKT) and the South Pole-Aitken (SPA). The PKT, which includes all the units with Th abundances higher than 3.5 ppm (KREEP-rich materials), is delimited by an almost continuous ringlike unit. In particular, it includes the western nearside maria, except for Mare Humorum. With concentrations in Fe, Ti, and Th enhanced relative to the surrounding highlands, the South Pole-Aitken basin floor represents a large mafic anomaly on the far side, suggesting wide deposits of lower crust and possible mantle materials. However, due to indirect residual latitude effects in the CSR (Clementine spectral reflectance) measurements, iron abundances might have been overestimated in SPA, thus implying that crustal materials, rather than mantle materials, might represent the dominant contributor to the mafic component exposed on the basin floor.

  8. Methicillin-Resistant Staphylococcus aureus spa Type t002 Outbreak in Horses and Staff at a Veterinary Teaching Hospital after Its Presumed Introduction by a Veterinarian.

    PubMed

    Steinman, Amir; Masarwa, Samira; Tirosh-Levy, Sharon; Gleser, Dan; Kelmer, Gal; Adler, Amos; Carmeli, Yehuda; Schwaber, Mitchell J

    2015-09-01

    Methicillin-resistant Staphylococcus aureus (MRSA) infection and colonization, involving MRSA strains which differ from common human health care-associated clones, have become serious emerging conditions in equine veterinary hospitals. In 2010, MRSA spa type t535 caused an outbreak involving both horses and personnel in a veterinary teaching hospital in Israel. Since then, surveillance continued, and occasional MRSA isolation occurred. Two years later, MRSA of another spa type, t002, was isolated from a veterinarian and, 3 weeks later, from a horse. The appearance of spa type t002, a common clone in human medicine in Israel, among both personnel and horses, prompted a point-prevalence survey of hospital personnel and hospitalized horses. Fifty-nine staff members (n = 16 equine; n = 43, other) and 14 horses were screened. Ten of 59 staff members (16.9%) and 7 of 14 horses (50%) were MRSA carriers. Among the staff, 44% of large animal department (LAD) personnel, compared with only 7% of non-LAD personnel, were carriers. Isolates from all horses and from 9 of 10 personnel were found to be of MRSA spa type t002. This clone was later isolated from an infected postoperative wound in a hospitalized horse. Measures were taken to contain transmission between horses and personnel, as was done in the previous outbreak, resulting in reduction of transmission and, finally, cessation of cross-transmission between horses and personnel.

  9. From Source Water to Tap Water to Spa and Swimming Pool Water: Effects of Disinfectanta and Precursors and Implications for Exposure and Toxicity

    EPA Science Inventory

    Introduction The current study investigated the effect of different disinfection treatments on the disinfection by-products (DBPs) formed in finished drinking water vs. tap water vs. swimming pool water vs. spa waters. To this end, samples across the complete water pathway (untr...

  10. The Recreational Water Cycle: From Source Water to Tap Water to Spa and Swimming Pool Water: Effects of Disinfectants and Precursors and Implications for Exposure and Toxicity

    EPA Science Inventory

    The current study investigates the effect of different disinfection treatments on the disinfection by-products (DBPs) formed in finished drinking water vs. tap water vs. swimming pool water vs. spa waters. To this end, complete water pathway samples (untreated source waters ->fi...

  11. Methicillin-Resistant Staphylococcus aureus spa Type t002 Outbreak in Horses and Staff at a Veterinary Teaching Hospital after Its Presumed Introduction by a Veterinarian

    PubMed Central

    Masarwa, Samira; Tirosh-Levy, Sharon; Gleser, Dan; Kelmer, Gal; Adler, Amos; Carmeli, Yehuda; Schwaber, Mitchell J.

    2015-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) infection and colonization, involving MRSA strains which differ from common human health care-associated clones, have become serious emerging conditions in equine veterinary hospitals. In 2010, MRSA spa type t535 caused an outbreak involving both horses and personnel in a veterinary teaching hospital in Israel. Since then, surveillance continued, and occasional MRSA isolation occurred. Two years later, MRSA of another spa type, t002, was isolated from a veterinarian and, 3 weeks later, from a horse. The appearance of spa type t002, a common clone in human medicine in Israel, among both personnel and horses, prompted a point-prevalence survey of hospital personnel and hospitalized horses. Fifty-nine staff members (n = 16 equine; n = 43, other) and 14 horses were screened. Ten of 59 staff members (16.9%) and 7 of 14 horses (50%) were MRSA carriers. Among the staff, 44% of large animal department (LAD) personnel, compared with only 7% of non-LAD personnel, were carriers. Isolates from all horses and from 9 of 10 personnel were found to be of MRSA spa type t002. This clone was later isolated from an infected postoperative wound in a hospitalized horse. Measures were taken to contain transmission between horses and personnel, as was done in the previous outbreak, resulting in reduction of transmission and, finally, cessation of cross-transmission between horses and personnel. PMID:26085620

  12. A New Quiet GSN Site at the South Pole: Comparison of Seismic Data Between SPA and QSPA.

    NASA Astrophysics Data System (ADS)

    Anderson, K. R.; Aster, R. C.; Butler, R.; Hutt, C.; Storm, T.; Anderson, D.; Vineyard, J. J.; Albert, D. G.

    2003-12-01

    Due to increasing noise from Amundsen-Scott South Pole Station (ASSPS), a new Global Seismic Network (GSN) station, QSPA, was constructed in the 2002-2003 field season to supplant the previous GSN station, SPA, in operation since c. 1985. QSPA is the first experiment established at the South Pole Remote Earth Science and Seismological Observatory (SPRESSO). The new instruments reside in the newly-designated seismic Quiet Sector, 8 km southeast of ASSPS. SPRESSO provides a much lower noise environment, yet is close enough to the south pole to be effectively co-sited with Earth's rotational axis for long-period seismological purposes. The SPRESSO site was selected after examining tractor surface noise propagation, balanced against power and communication considerations. Numerical modeling showed that ASSPS noise should be fairly well trapped in near-surface, lower-velocity firn zone and indicated that a burial depth of 300 m would very substantially reduce noise. QSPA consists of a Geotech Instruments KS-54000 at 275 m and Guralp CMG-3Tb at 255 m depth, separated horizontally by 10 m. These depths are approximately 160 m below the local firn/ice transition. QSPA also includes a near-surface vault ( ˜5 m depth) housing Streckeisen STS-1V and STS-2 sensors. SPA has been left in operation for a period of ˜1 yr to allow for a thorough comparison with QSPA. Although 8 km from the South Pole station activities, the QSPA site borehole instruments still sense South Pole noise. However, these noise levels are significantly diminished at frequencies >1 Hz and at periods >20 s. From 1 to 15 Hz, the improved noise environment is especially dramatic. For example, background noise at 2 Hz is 15 db below SPA, ˜20-25 dB at 3 Hz, ˜30-35 dB at 4Hz, and ˜35-40 dB above 5Hz. Between 2 and 10 Hz the QSPA Guralp borehole sensor shows data intervals with noise levels below the Peterson (1993) Low Noise Model (PLNM) (with minimum noise at ˜3Hz ˜12dB below PLNM), making the site among

  13. Devonian shelf basin, Michigan basin, Alpena region

    SciTech Connect

    Gutschick, R.C.

    1986-08-01

    This biostratigraphic study involves the Devonian paleogeography-paleoecology-paleobathymetry of the transition from carbonate platform shelf margin to basinal sedimentation for the northern part of the Michigan basin in the Alpena region. Shelf-basin analysis is based on lithofacies, rock colors, concretion, biostratigraphy, paleoecology of faunas - especially microfaunas and trace fossils - stratified water column, eustasy, and application of Walther's Law. Field observations were made on Partridge Point along Lake Huron, where type sections of the Middle Devonian Thunder Bay Limestone and Late Devonian Squaw Bay Limestone are exposed; and the Antrim black shale at Paxton quarry. The Thunder Bay Limestone evolved as a carbonate platform, subtidal shelf-margin aerobic environment dominated by sessile benthic coralline organisms and shelly fauna, but not reef framework. The Squaw Bay Limestone is transitional shelf to basin, with aspects of slope environment and deeper water off-platform, pelagic organic biostromal molluscan-conodont carbonate deposited during the onset of a stratified water column (dysaerobic benthos-polychaete. agglutinated tubes, sulfides) and pycnocline. The Antrim Shale, in an exceptional black shale exposure in the Paxton quarry, represents deep-water basinal deposition whose bottom waters lacked oxygen. Faunas (conodonts, styliolines, radiolarians) and floras (tasmanitids, calamitids, palynomorphs) are from the aerobic pelagic realm, as indicated from concretions and shale fossil evidence. A benthos is lacking, except for bioturbation from organisms introduced by entrained oxygenated distal turbidite dispersion into the barren bottom black muds. Basinal hydrocarbon source rocks are abundant and updip carbonate reservoirs rim the basin. The Antrim Shale sequence contains the interval of Frasnian-Famennian faunal extinction.

  14. Origin of cratonic basins

    SciTech Connect

    de V. Klein, G.; Hsui, A.T.

    1987-12-01

    Tectonic subsidence curves show that the Illinois, Michigan, and Williston basins formed by initial fault-controlled mechanical subsidence during rifting and by subsequent thermal subsidence. Thermal subsidence began around 525 Ma in the Illinois Basin, 520-460 Ma in the Michigan Basin, and 530-500 Ma in the Williston Basin. In the Illinois Basin, a second subsidence episode (middle Mississippian through Early Permian) was caused by flexural foreland subsidence in response to the Alleghanian-Hercynian orogeny. Past workers have suggested mantle phase changes at the base of the crust, mechanical subsidence in response to isostatically uncompensated excess mass following igneous intrusions, intrusion of mantle plumes into the crust, or regional thermal metamorphic events as causes of basin initiation. Cratonic basins of North America, Europe, Africa, and South America share common ages of formation, histories of sediment accumulation, temporal volume changes of sediment fills, and common dates of interregional unconformities. Their common date of formation suggests initiation of cratonic basins in response to breakup of a late Precambrian supercontinent. This supercontinent acted as a heat lens that caused partial melting of the lower crust and upper mantle followed by emplacement of anorogenic granites during extensional tectonics in response to supercontinent breakup. Intrusion of anorogenic granites and other partially melted intrusive rocks weakened continental lithosphere, thus providing a zone of localized regional stretching and permitting formation of cratonic basins almost simultaneously over sites of intrusion of these anorogenic granites and other partially melted intrusive rocks.

  15. Validation of the Spanish Version of the Eating Assessment Tool-10 (EAT-10spa) in Colombia. A Blinded Prospective Cohort Study.

    PubMed

    Giraldo-Cadavid, Luis Fernando; Gutiérrez-Achury, Alejandra María; Ruales-Suárez, Karem; Rengifo-Varona, Maria Leonor; Barros, Camilo; Posada, Andrés; Romero, Carlos; Galvis, Ana María

    2016-06-01

    Dysphagia might affect 12 % of the general population, and its complications include pneumonia, malnutrition, social isolation, and death. No validated Spanish symptom survey exists to quantify dysphagia symptoms among Latin Americans. Therefore, we performed a prospective cohort study in a tertiary care university hospital to validate the Spanish version of the 10-Item Eating-Assessment-Tool (EAT-10spa) for use in Colombia. After an interdisciplinary committee of five bilingual specialists evaluated the EAT-10spa (translated and validated in Spain) and deemed it appropriate for the Colombian culture, its feasibility, reliability, validity, sensitivity to change, and diagnostic capacity were evaluated. As a reference standard, we used the flexible endoscopic evaluation of swallowing with sensory testing and a standardized clinical evaluation. All assessments were blinded. In total, 133 subjects were included (52 % women, mean age 55 years) and completed the EAT-10spa (median completion time: 2 min [IQR 1-3 min]), 39 % of whom had an elementary-level education. Cronbach's α coefficient: 0.91; test-retest intra-class correlation coefficient: 0.94. The Spearman's correlation coefficient of the EAT-10spa with the 8-point penetration-aspiration scale was 0.54 (P < 0.001). The area under the receiver-operating-characteristic-curve (AUC-ROC) for dysphagia and aspiration were 0.79 (P < 0.001) and 0.81 (P < 0.001), respectively. The best cut-off points for dysphagia and aspiration were EAT-10spa ≥2 (sensitivity 93.6 %, specificity 36.4 %) and EAT-10spa ≥4 (sensitivity 94.3 %, specificity 49.5 %), respectively. A reduction in the EAT-10 ≥3 was the best cut-off point for a clinically significant improvement (AUC-ROC 0.83; P < 0.0001). The EAT-10spa showed excellent psychometric properties and discriminatory capacity for use in Colombia.

  16. [The development of the medical information system for the improvement of the quality of work of the Crimean spa and health resorts].

    PubMed

    Ezhov, V V; Grigor'ev, P E; Mizin, V I; Andriyashek, Yu I; Gol'dberg, D L; Olenchuk, A V

    2016-01-01

    The Crimea has the enormous potential for the health promotion activities. However, neither the profile of these activities nor the demand for the socio-medical services is clearly defined for the majority of the local spa and health resort facilities. The possibilities of modern information technology are not used in the full measure either. The objective of the present work was to elaborate the new medical information system and demonstrate its effectiveness. In addition, the article describes the main advantages of the system for the optimization of healthcare in the Crimean spa and health resort facilities. We reviewed and analyzed various literature publications, legal framework, standards, regulations, guidelines, and questionnaire survey data obtain at 50 spa and health resort facilities of the Crimea. The results of the assessment indicate the necessity of the systematic approach to the analysis of the quality of medical care and the process of its further development. Statistical and mathematical methods were used to elaborate the medical information system for the optimization of the activities of the Crimean spa and health resorts. The distinctive features of the proposed information system are modularity and the possibility of flexible adjustment to the conditions of individual settings, one-step data loading with the subsequent multiple application for the formulation of documents, automated filling of records in compliance with the medical standards, and taking into consideration the possible changes in or amendments to the form of the documents. The data obtained in the course of project implementation were used for the first time in the Republic of Crimea to design, substantiate, and recommend for the practical application the algorithm for the comprehensive estimation of the results of treatment of the patients based at the spa and health resort facilities with due regard for the specific regional conditions.

  17. Aerosolization of Respirable Droplets from a Domestic Spa Pool and the Use of MS-2 Coliphage and Pseudomonas aeruginosa as Markers for Legionella pneumophila

    PubMed Central

    Hewitt, Matthew; Stevenson, David; Walker, Jimmy T.; Bennett, Allan M.

    2014-01-01

    Legionnaires' disease can result when droplets or aerosols containing legionella bacteria are inhaled and deposited in the lungs. A number of outbreaks have been associated with the use of a spa pool where aeration, a high water temperature, and a large and variable organic load make disinfectant levels difficult to maintain. Spa pool ownership is increasing, and the aim of this study, using two surrogate organisms (MS-2 coliphage and Pseudomonas aeruginosa [a natural contaminant]), was to assess the potential risk to domestic users when disinfection fails. A representative “entry level” domestic spa pool was installed in an outdoor courtyard. The manufacturer's instructions for spa pool maintenance were not followed. A cyclone sampler was used to sample the aerosols released from the spa pool with and without activation of the air injection system. Samples were taken at increasing heights and distances from the pool. An aerodynamic particle sizer was used to measure the water droplet size distribution at each sample point. When the air injection system was inactivated, neither surrogate organism was recovered from the air. On activation of the air injection system, the mean mass of droplets within the respirable range (10 cm above the water line) was 36.8 μg cm−3. This corresponded to a mean air concentration of P. aeruginosa of 350 CFU m−3. From extrapolation from animal data, the estimated risk of infection from aerosols contaminated with similar concentrations of Legionella pneumophila was 0.76 (males) and 0.65 (females). At 1 m above and/or beyond the pool, the mean aerosol mass decreased to 0.04 μg cm−3 and corresponded to a 100-fold reduction in mean microbial air concentration. The estimated risk of infection at this distance was negligible. PMID:25381233

  18. High frequency of multidrug-resistant Staphylococcus aureus with SCCmec type III and Spa types t037 and t631 isolated from burn patients in southwest of Iran.

    PubMed

    Parhizgari, Najmeh; Khoramrooz, Seyed Sajjad; Malek Hosseini, Seyed Ali Asghar; Marashifard, Masoud; Yazdanpanah, Mahboobeh; Emaneini, Mohammad; Gharibpour, Farzaneh; Mirzaii, Mehdi; Darban-Sarokhalil, Davood; Moein, Masoud; Naraki, Mahmood

    2016-03-01

    Methicilin resistance Staphylococcus aureus (MRSA) infections are the major challenges in hospitals, especially in the burn units. The use of molecular typing methods is essential for tracking the spread of S. aureus infection and epidemiological investigations. The aim of this study was to find the profile of the spa types and also the prevalence of each SCCmec type of S. aureus strains in a central burn hospital in southwest of Iran. A total of 81 non-duplicate S. aureus were isolated from burn patients between April 2011 and February 2012. The susceptibility of the isolates against 13 different antibiotics was tested by disk agar diffusion (DAD) method. MRSA strains were identified by amplification of mecA gene. Multiplex-polymerase chain reaction (PCR) technique was used to determine the SCCmec types of MRSA strains and all the S. aureus isolates were typed by spa typing method. Detection of mecA gene showed that 70 (86.4%) of the isolates were MRSA. The highest rate of resistance was observed for penicillin (97.5%) and erythromycin (77.8%). None of the isolates were resistant to vancomycin. Sixty-seven of the 70 MRSA isolates harbored only SCCmec type III and three untypeable isolates. Five different spa types were detected. The most common spa types were t037 (42.5%) and t631 (34.5%) and were only found in MRSA isolates. Only SCCmec type III was found in burn patients which emphasizes the HA-MRSA origin of these strains. Only five different spa types identified in this study are in accordance with one SCCmec type which indicates that a limited number of bacterial colons are circulated in the burn unit in this hospital.

  19. Scintillation proximity assay (SPA) as a new approach to determine a ligand's kinetic profile. A case in point for the adenosine A1 receptor.

    PubMed

    Xia, Lizi; de Vries, Henk; IJzerman, Ad P; Heitman, Laura H

    2016-03-01

    Scintillation proximity assay (SPA) is a radio-isotopic technology format used to measure a wide range of biological interactions, including drug-target binding affinity studies. The assay is homogeneous in nature, as it relies on a "mix and measure" format. It does not involve a filtration step to separate bound from free ligand as is the case in a traditional receptor-binding assay. For G protein-coupled receptors (GPCRs), it has been shown that optimal binding kinetics, next to a high affinity of a ligand, can result in more desirable pharmacological profiles. However, traditional techniques to assess kinetic parameters tend to be cumbersome and laborious. We thus aimed to evaluate whether SPA can be an alternative platform for real-time receptor-binding kinetic measurements on GPCRs. To do so, we first validated the SPA technology for equilibrium binding studies on a prototypic class A GPCR, the human adenosine A1 receptor (hA1R). Differently to classic kinetic studies, the SPA technology allowed us to study binding kinetic processes almost real time, which is impossible in the filtration assay. To demonstrate the reliability of this technology for kinetic purposes, we performed the so-called competition association experiments. The association and dissociation rate constants (k on and k off) of unlabeled hA1R ligands were reliably and quickly determined and agreed very well with the same parameters from a traditional filtration assay performed simultaneously. In conclusion, SPA is a very promising technique to determine the kinetic profile of the drug-target interaction. Its robustness and potential for high-throughput may render this technology a preferred choice for further kinetic studies.

  20. Progressive Increase in Disinfection Byproducts and Mutagenicity from Source to Tap to Swimming Pool and Spa Water: Impact of Human Inputs.

    PubMed

    Daiber, Eric J; DeMarini, David M; Ravuri, Sridevi A; Liberatore, Hannah K; Cuthbertson, Amy A; Thompson-Klemish, Alexis; Byer, Jonathan D; Schmid, Judith E; Afifi, Mehrnaz Z; Blatchley, Ernest R; Richardson, Susan D

    2016-07-01

    Pools and spas are enjoyed throughout the world for exercise and relaxation. However, there are no previous studies on mutagenicity of disinfected spa (hot tub) waters or comprehensive identification of disinfection byproducts (DBPs) formed in spas. Using 28 water samples from seven sites, we report the first integrated mutagenicity and comprehensive analytical chemistry of spas treated with chlorine, bromine, or ozone, along with pools treated with these same disinfectants. Gas chromatography (GC) with high-resolution mass spectrometry, membrane-introduction mass spectrometry, and GC-electron capture detection were used to comprehensively identify and quantify DBPs and other contaminants. Mutagenicity was assessed by the Salmonella mutagenicity assay. More than 100 DBPs were identified, including a new class of DBPs, bromoimidazoles. Organic extracts of brominated pool/spa waters were 1.8× more mutagenic than chlorinated ones; spa waters were 1.7× more mutagenic than pools. Pool and spa samples were 2.4 and 4.1× more mutagenic, respectively, than corresponding tap waters. The concentration of the sum of 21 DBPs measured quantitatively increased from finished to tap to pool to spa; and mutagenic potency increased from finished/tap to pools to spas. Mutagenic potencies of samples from a chlorinated site correlated best with brominated haloacetic acid concentrations (Br-HAAs) (r = 0.98) and nitrogen-containing DBPs (N-DBPs) (r = 0.97) and the least with Br-trihalomethanes (r = 0.29) and Br-N-DBPs (r = 0.04). The mutagenic potencies of samples from a brominated site correlated best (r = 0.82) with the concentrations of the nine HAAs, Br-HAAs, and Br-DBPs. Human use increased significantly the DBP concentrations and mutagenic potencies for most pools and spas. These data provide evidence that human precursors can increase mutagenic potencies of pools and spas and that this increase is associated with increased DBP concentrations.

  1. Scintillation proximity assay (SPA) as a new approach to determine a ligand's kinetic profile. A case in point for the adenosine A1 receptor.

    PubMed

    Xia, Lizi; de Vries, Henk; IJzerman, Ad P; Heitman, Laura H

    2016-03-01

    Scintillation proximity assay (SPA) is a radio-isotopic technology format used to measure a wide range of biological interactions, including drug-target binding affinity studies. The assay is homogeneous in nature, as it relies on a "mix and measure" format. It does not involve a filtration step to separate bound from free ligand as is the case in a traditional receptor-binding assay. For G protein-coupled receptors (GPCRs), it has been shown that optimal binding kinetics, next to a high affinity of a ligand, can result in more desirable pharmacological profiles. However, traditional techniques to assess kinetic parameters tend to be cumbersome and laborious. We thus aimed to evaluate whether SPA can be an alternative platform for real-time receptor-binding kinetic measurements on GPCRs. To do so, we first validated the SPA technology for equilibrium binding studies on a prototypic class A GPCR, the human adenosine A1 receptor (hA1R). Differently to classic kinetic studies, the SPA technology allowed us to study binding kinetic processes almost real time, which is impossible in the filtration assay. To demonstrate the reliability of this technology for kinetic purposes, we performed the so-called competition association experiments. The association and dissociation rate constants (k on and k off) of unlabeled hA1R ligands were reliably and quickly determined and agreed very well with the same parameters from a traditional filtration assay performed simultaneously. In conclusion, SPA is a very promising technique to determine the kinetic profile of the drug-target interaction. Its robustness and potential for high-throughput may render this technology a preferred choice for further kinetic studies. PMID:26647040

  2. Aerosolization of respirable droplets from a domestic spa pool and the use of MS-2 coliphage and Pseudomonas aeruginosa as markers for Legionella pneumophila.

    PubMed

    Moore, Ginny; Hewitt, Matthew; Stevenson, David; Walker, Jimmy T; Bennett, Allan M

    2015-01-01

    Legionnaires' disease can result when droplets or aerosols containing legionella bacteria are inhaled and deposited in the lungs. A number of outbreaks have been associated with the use of a spa pool where aeration, a high water temperature, and a large and variable organic load make disinfectant levels difficult to maintain. Spa pool ownership is increasing, and the aim of this study, using two surrogate organisms (MS-2 coliphage and Pseudomonas aeruginosa [a natural contaminant]), was to assess the potential risk to domestic users when disinfection fails. A representative "entry level" domestic spa pool was installed in an outdoor courtyard. The manufacturer's instructions for spa pool maintenance were not followed. A cyclone sampler was used to sample the aerosols released from the spa pool with and without activation of the air injection system. Samples were taken at increasing heights and distances from the pool. An aerodynamic particle sizer was used to measure the water droplet size distribution at each sample point. When the air injection system was inactivated, neither surrogate organism was recovered from the air. On activation of the air injection system, the mean mass of droplets within the respirable range (10 cm above the water line) was 36.8 μg cm(-3). This corresponded to a mean air concentration of P. aeruginosa of 350 CFU m(-3). From extrapolation from animal data, the estimated risk of infection from aerosols contaminated with similar concentrations of Legionella pneumophila was 0.76 (males) and 0.65 (females). At 1 m above and/or beyond the pool, the mean aerosol mass decreased to 0.04 μg cm(-3) and corresponded to a 100-fold reduction in mean microbial air concentration. The estimated risk of infection at this distance was negligible.

  3. Progressive Increase in Disinfection Byproducts and Mutagenicity from Source to Tap to Swimming Pool and Spa Water: Impact of Human Inputs.

    PubMed

    Daiber, Eric J; DeMarini, David M; Ravuri, Sridevi A; Liberatore, Hannah K; Cuthbertson, Amy A; Thompson-Klemish, Alexis; Byer, Jonathan D; Schmid, Judith E; Afifi, Mehrnaz Z; Blatchley, Ernest R; Richardson, Susan D

    2016-07-01

    Pools and spas are enjoyed throughout the world for exercise and relaxation. However, there are no previous studies on mutagenicity of disinfected spa (hot tub) waters or comprehensive identification of disinfection byproducts (DBPs) formed in spas. Using 28 water samples from seven sites, we report the first integrated mutagenicity and comprehensive analytical chemistry of spas treated with chlorine, bromine, or ozone, along with pools treated with these same disinfectants. Gas chromatography (GC) with high-resolution mass spectrometry, membrane-introduction mass spectrometry, and GC-electron capture detection were used to comprehensively identify and quantify DBPs and other contaminants. Mutagenicity was assessed by the Salmonella mutagenicity assay. More than 100 DBPs were identified, including a new class of DBPs, bromoimidazoles. Organic extracts of brominated pool/spa waters were 1.8× more mutagenic than chlorinated ones; spa waters were 1.7× more mutagenic than pools. Pool and spa samples were 2.4 and 4.1× more mutagenic, respectively, than corresponding tap waters. The concentration of the sum of 21 DBPs measured quantitatively increased from finished to tap to pool to spa; and mutagenic potency increased from finished/tap to pools to spas. Mutagenic potencies of samples from a chlorinated site correlated best with brominated haloacetic acid concentrations (Br-HAAs) (r = 0.98) and nitrogen-containing DBPs (N-DBPs) (r = 0.97) and the least with Br-trihalomethanes (r = 0.29) and Br-N-DBPs (r = 0.04). The mutagenic potencies of samples from a brominated site correlated best (r = 0.82) with the concentrations of the nine HAAs, Br-HAAs, and Br-DBPs. Human use increased significantly the DBP concentrations and mutagenic potencies for most pools and spas. These data provide evidence that human precursors can increase mutagenic potencies of pools and spas and that this increase is associated with increased DBP concentrations. PMID:27124361

  4. [The development of the medical information system for the improvement of the quality of work of the Crimean spa and health resorts].

    PubMed

    Ezhov, V V; Grigor'ev, P E; Mizin, V I; Andriyashek, Yu I; Gol'dberg, D L; Olenchuk, A V

    2016-01-01

    The Crimea has the enormous potential for the health promotion activities. However, neither the profile of these activities nor the demand for the socio-medical services is clearly defined for the majority of the local spa and health resort facilities. The possibilities of modern information technology are not used in the full measure either. The objective of the present work was to elaborate the new medical information system and demonstrate its effectiveness. In addition, the article describes the main advantages of the system for the optimization of healthcare in the Crimean spa and health resort facilities. We reviewed and analyzed various literature publications, legal framework, standards, regulations, guidelines, and questionnaire survey data obtain at 50 spa and health resort facilities of the Crimea. The results of the assessment indicate the necessity of the systematic approach to the analysis of the quality of medical care and the process of its further development. Statistical and mathematical methods were used to elaborate the medical information system for the optimization of the activities of the Crimean spa and health resorts. The distinctive features of the proposed information system are modularity and the possibility of flexible adjustment to the conditions of individual settings, one-step data loading with the subsequent multiple application for the formulation of documents, automated filling of records in compliance with the medical standards, and taking into consideration the possible changes in or amendments to the form of the documents. The data obtained in the course of project implementation were used for the first time in the Republic of Crimea to design, substantiate, and recommend for the practical application the algorithm for the comprehensive estimation of the results of treatment of the patients based at the spa and health resort facilities with due regard for the specific regional conditions. PMID:27030569

  5. Solar-energy-system performance-evaluation update: Wood Road School, Ballston Spa, New York, October 1982-April 1983

    SciTech Connect

    Kendall, P

    1983-01-01

    The Wood Road School Solar Project is a 216,000 square foot combined elementary and middle school in Ballston Spa, New York. The solar energy system supplies energy to the space heating and domestic hot water subsystems. Heat is collected by flat plate collector panels and stored in two storage tanks. Performance data are given for the system overall and for each of the four subsystems - energy collection, storage, space heating, and domestic hot water. Data are also provided on operating energy, energy savings, and weather conditions. Design and actual system solar fraction are compared, and percentage of incident solar energy and collected solar energy utilized are given. Also given are building loads analysis, system thermal losses, and system coefficient of performance. (LEW)

  6. Origin of cratonic basins

    NASA Astrophysics Data System (ADS)

    Dev. Klein, George; Hsui, Albert T.

    1987-12-01

    Tectonic subsidence curves show that the Illinois, Michigan, and Williston basins formed by initial fault-controlled mechanical subsidence during rifting and by subsequent thermal subsidence. Thermal subsidence began around 525 Ma in the Illinois Basin, 520 460 Ma in the Michigan Basin, and 530 500 Ma in the Williston Basin. In the Illinois Basin, a second subsidence episode (middle Mississippian through Early Permian) was caused by flexural foreland subsidence in response to the Alleghanian-Hercynian orogeny. Resurgent Permian rifting in the Illinois Basin is inferred because of intrusion of well-dated Permian alnoites; such intrusive rocks are normally associated with rifting processes. The process of formation of these cratonic basins remains controversial. Past workers have suggested mantle phase changes at the base of the crust, mechanical subsidence in response to isostatically uncompensated excess mass following igneous intrusions, intrusion of mantle plumes into the crust, or regional thermal metamorphic events as causes of basin initiation. Cratonic basins of North America, Europe, Africa, and South America share common ages of formation (around 550 to 500 Ma), histories of sediment accumulation, temporal volume changes of sediment fills, and common dates of interregional unconformities. Their common date of formation suggests initiation of cratonic basins in response to breakup of a late Precambrian super-continent. This supercontinent acted as a heat lens that caused partial melting of the lower crust and upper mantle followed by emplacement of anorogenic granites during extensional tectonics in response to supercontinent breakup. Intrusion of anorogenic granites and other partially melted intrusive rocks weakened continental lithosphere, thus providing a zone of localized regional stretching and permitting formation of cratonic basins almost simultaneously over sites of intrusion of these anorogenic granites and other partially melted intrusive rocks.

  7. Intra-arc basins

    SciTech Connect

    Smith, G.A.

    1988-01-01

    Convergent-margin tectonic models feature forearc and back-arc basins and generally portray the arc itself as structurally static. However, intra-arc tectonics not only control distribution and petrology of extrusives and plutons, but also generate basins along the magmatic axis. Magma withdrawal and crustal loading by volcanic edifices contribute to subsidence, but most intra-arc basins are grabens or half-grabens indicative of extension. Grabens are isolated or continuous along long segments of the arc. Basin development may alternate with periods of arc uplife. No unique set of conditions causes intra-arc extension; numerous scenarios may initiate extension and subsidence of thermally weakened arc crust. Transtension related to oblique convergence contributed to the formation of most modern intra-arc basins. Andean basins may result from gravitational spreading of an unusually highstanding arc. Intra-arc basin sediment traps may starve arc-adjacent basins from coarse volcaniclastic detritus. Terrestrial intra-arc basins accommodate thick volcanic and volcaniclastic sediment sections, including lacustrine sequences. Marine intra-arc basins include bounding carbonate shelves, marginal and local intrabasinal submarine fans and aprons, and basin plains receiving pelagic and hemipelagic sediments. Structural patterns are appropriate for trapping hydrocarbons, source rocks are commonly present, and high heat flow favors early maturation. Reservoir quality is typically poor because of volcaniclastic diagenesis, but secondary porosity from dissolution of framework feldspars and carbonate or laumontite cements, and the known productivity of some volcanic reservoirs, suggest the potential for hydrocarbon accumulations. Geothermal resources and modest coal potential have also been recognized.

  8. The use of spa and phage typing for characterization of clinical isolates of methicillin-resistant Staphylococcus aureus in the University Clinical Center in Gdańsk, Poland.

    PubMed

    Wiśniewska, Katarzyna; Szewczyk, Anna; Piechowicz, Lidia; Bronk, Marek; Samet, Alfred; Swieć, Krystyna

    2012-05-01

    The emergence of spa types and spa-clonal complexes (CC) among clinical methicillin-resistant Staphylococcus aureus isolates collected from the University Clinical Center in Gdańsk between 2008 and 2009 were investigated. Phage typing was used as the initial screening in the study. The basic set of phages and the additional set of phages were used. Most of the isolates (56 %) belonged to the phage group III. With the additional set of phages, eight types were found, with predominant one MR8 (50 %). Sixteen distinct spa types were observed. The most frequent were t003 (22 %), t151 (16 %), and t008 (12 %). The spa types were clustered into two spa-CC and eight singletons. The predominant CC010 (50 %) consisted of six types, with the most common t003 (36.7 %) and t151(26.7 %), and in 80 % was identified as staphylococcal chromosomal casette mec (SCCmec) type II. The second cluster has no founder (12 %) with only two spa types: t037 belonging to SCCmec type III and t029. In the most frequent singleton, spa type t008 alone was clustered in 12 % of the isolates. All singletons correspond to SCCmec type IV. The CC010 was distributed in most of the hospital wards, corresponded to Multilocus sequence typing type ST5/ST225 and was constantly present throughout the observed period. The isolates of CC010 generally belonged to the phage group III, and most of them (53.3 %) were resistant to erythromycin, clindamycin, and ciprofloxacin. The concordance between spa-clone and phage type was very high, but the same phage type MR8 was observed within different spa types of the predominant clone.

  9. Evaluating the usefulness of spa typing, in comparison with pulsed-field gel electrophoresis, for epidemiological typing of methicillin-resistant Staphylococcus aureus in a low-prevalence region in Sweden 2000-2004.

    PubMed

    Petersson, A C; Olsson-Liljequist, B; Miörner, H; Haeggman, S

    2010-05-01

    The usefulness of spa typing was evaluated in relation to pulsed-field gel electrophoresis (PFGE), as a tool for epidemiological typing of methicillin-resistant Staphylococcus aureus (MRSA) in a low-prevalence region in southern Sweden. Bacterial isolates from 216 MRSA cases, newly identified in 2000-2004, were studied. The isolates were obtained from infected patients (31%), and from colonized individuals found by screening (69%). In total, 49 spa types and 73 PFGE patterns were identified. The discriminatory power of spa typing was lower (94.9 +/- 1.8%) than that of PFGE (97.3 +/- 1.2%). For two spa types (t002 and t008) the Panton-Valentine leukocidin results added useful discriminatory information. The most common spa types were t044 (n = 31; four PFGE patterns), t002 (n = 24; 10 PFGE patterns), t067 (n = 12; four PFGE patterns), t050 (n = 12; one PFGE pattern), and t324 (n = 11; one PFGE pattern). Epidemiological investigations identified 91 single cases and 39 transmission chains, each involving two to 13 cases. All the transmission chains were held together both by spa and PFGE typing. Among the 91 single-case isolates, 33 spa types and 50 PFGE patterns were unique (matchless) at the time of identification. The low prevalence of MRSA, the low number of outbreaks, and the wide spectrum of strains due to frequent acquisitions abroad (49% of the cases), makes spa typing a useful complement to epidemiological investigations in our setting. However, we still recommend the continued use of PFGE for further discrimination of isolates with identical spa types when epidemiological data can not exclude possible transmission.

  10. Arabidopsis COP1 and SPA genes are essential for plant elongation but not for acceleration of flowering time in response to a low red light to far-red light ratio.

    PubMed

    Rolauffs, Sebastian; Fackendahl, Petra; Sahm, Jan; Fiene, Gabriele; Hoecker, Ute

    2012-12-01

    Plants sense vegetative shade as a reduction in the ratio of red light to far-red light (R:FR). Arabidopsis (Arabidopsis thaliana) responds to a reduced R:FR with increased elongation of the hypocotyl and the leaf petioles as well as with an acceleration of flowering time. The repressor of light signaling, CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1), has been shown previously to be essential for the shade-avoidance response in seedlings. Here, we have investigated the roles of COP1 and the COP1-interacting SUPPRESSOR OF PHYA-105 (SPA) proteins in seedling and adult facets of the shade-avoidance response. We show that COP1 and the four SPA genes are essential for hypocotyl and leaf petiole elongation in response to low R:FR, in a fashion that involves the COP1/SPA ubiquitination target LONG HYPOCOTYL IN FR LIGHT1 but not ELONGATED HYPOCOTYL5. In contrast, the acceleration of flowering in response to a low R:FR was normal in cop1 and spa mutants, thus demonstrating that the COP1/SPA complex is only required for elongation responses to vegetative shade and not for shade-induced early flowering. We further show that spa mutant seedlings fail to exhibit an increase in the transcript levels of the auxin biosynthesis genes YUCCA2 (YUC2), YUC8, and YUC9 in response to low R:FR, suggesting that an increase in auxin biosynthesis in vegetative shade requires SPA function. Consistent with this finding, expression of the auxin-response marker gene DR5::GUS did not increase in spa mutant seedlings exposed to low R:FR. We propose that COP1/SPA activity, via LONG HYPOCOTYL IN FR LIGHT1, is required for shade-induced modulation of the auxin biosynthesis pathway and thereby enhances cell elongation in low R:FR.

  11. A new model of lunar crust: asymmetry in crustal composition and evolution

    NASA Astrophysics Data System (ADS)

    Arai, Tomoko; Takeda, Hiroshi; Yamaguchi, Akira; Ohtake, Makiko

    2008-04-01

    Earlier models of lunar crustal formation as a simple flotation of ferroan anorthosites (FAN) do not account for the diverse crustal composition revealed by feldspathic lunar meteorites and granulites in the Apollo samples. Based on the integrated results of recent studies of lunar meteorites and global chemical and mineralogical maps, we propose a novel asymmetric crust model with a ferroan, noritic, nearside crust and a magnesian, troctolitic farside crust. Asymmetric crystallization of a primordial magma ocean can be one possibility to produce a crust with an asymmetric composition. A post-magma-ocean origin for a portion of the lunar crust is also possible and would account for the positive eNd value for FAN and phase equilibria. The formation of giant basins, such as the South Pole-Aitken (SPA) basin may have significant effects on resurfacing of the early lunar crust. Thus, the observed surface composition of the feldspathic highland terrane (FHT) represents the combined results of magma ocean crystallization, post-magma-ocean magmatism and resurfacing by basin formation. The Mg/(Mg+Fe) ratios, rock types, and mineral compositions of the FHT and the South Pole-Aitken basin Terrane (SPAT) obtained from the KAGUYA mission, coupled with further mineralogical and isotopic studies of lunar meteorites, will facilitate an assessment of the feasibility of the proposed crust model and improve understanding of lunar crustal genesis and evolution.

  12. Reserves in western basins

    SciTech Connect

    Caldwell, R.H.; Cotton, B.W.

    1995-04-01

    The objective of this project is to investigate the reserves potential of tight gas reservoirs in three Rocky Mountain basins: the Greater Green River (GGRB), Uinta and Piceance basins. The basins contain vast gas resources that have been estimated in the thousands of Tcf hosted in low permeability clastic reservoirs. This study documents the productive characteristics of these tight reservoirs, requantifies gas in place resources, and characterizes the reserves potential of each basin. The purpose of this work is to promote understanding of the resource and to encourage its exploitation by private industry. At this point in time, the GGRB work has been completed and a final report published. Work is well underway in the Uinta and Piceance basins which are being handled concurrently, with reports on these basins being scheduled for the middle of this year. Since the GGRB portion of the project has been completed, this presentation win focus upon that basin. A key conclusion of this study was the subdivision of the resource, based upon economic and technological considerations, into groupings that have distinct properties with regard to potential for future producibility, economics and risk profile.

  13. The protective effect of different airway humidification liquids to lung after tracheotomy in traumatic brain injury: The role of pulmonary surfactant protein-A (SP-A).

    PubMed

    Su, Xinyang; Li, Zefu; Wang, Meilin; Li, Zhenzhu; Wang, Qingbo; Lu, Wenxian; Li, Xiaoli; Zhou, Youfei; Xu, Hongmei

    2016-02-10

    The purpose of this study was to establish a rat model of a brain injury with tracheotomy and compared the wetting effects of different airway humidification liquids, afterward, the best airway humidification liquid was selected for the clinical trial, thus providing a theoretical basis for selecting a proper airway humidification liquid in a clinical setting. Rats were divided into a sham group, group A (0.9% NaCl), group B (0.45% NaCl), group C (0.9% NaCl+ambroxol) and group D (0.9% NaCl+Pulmicort). An established rat model of traumatic brain injury with tracheotomy was used. Brain tissue samples were taken to determine water content, while lung tissue samples were taken to determine wet/dry weight ratio (W/D), histological changes and expression levels of SP-A mRNA and SP-A protein. 30 patients with brain injury and tracheotomy were selected and divided into two groups based on the airway humidification liquid instilled in the trachea tube, 0.45% NaCl and 0.9% NaCl+ambroxol. Blood was then extracted from the patients to measure the levels of SP-A, interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor-α (TNF-α). The difference between group C and other groups in lung W/D and expression levels of SP-A mRNA and SP-A protein was significant (P<0.05). In comparison, the histological changes showed that the lung tissue damage was smallest in group C compared to the three other groups. Aspect of patients, 0.45% NaCl group and 0.9% NaCl+ambroxol group were significantly different in the levels of SP-A, IL-6, IL-8 and TNF-α (P<0.01). In the present study, 0.9% NaCl+ambroxol promote the synthesis and secretion of pulmonary surfactant, and has anti-inflammatory and antioxidant effects, which inhibit the release of inflammatory factors and cytokines, making it an ideal airway humidification liquid.

  14. The protective effect of different airway humidification liquids to lung after tracheotomy in traumatic brain injury: The role of pulmonary surfactant protein-A (SP-A).

    PubMed

    Su, Xinyang; Li, Zefu; Wang, Meilin; Li, Zhenzhu; Wang, Qingbo; Lu, Wenxian; Li, Xiaoli; Zhou, Youfei; Xu, Hongmei

    2016-02-10

    The purpose of this study was to establish a rat model of a brain injury with tracheotomy and compared the wetting effects of different airway humidification liquids, afterward, the best airway humidification liquid was selected for the clinical trial, thus providing a theoretical basis for selecting a proper airway humidification liquid in a clinical setting. Rats were divided into a sham group, group A (0.9% NaCl), group B (0.45% NaCl), group C (0.9% NaCl+ambroxol) and group D (0.9% NaCl+Pulmicort). An established rat model of traumatic brain injury with tracheotomy was used. Brain tissue samples were taken to determine water content, while lung tissue samples were taken to determine wet/dry weight ratio (W/D), histological changes and expression levels of SP-A mRNA and SP-A protein. 30 patients with brain injury and tracheotomy were selected and divided into two groups based on the airway humidification liquid instilled in the trachea tube, 0.45% NaCl and 0.9% NaCl+ambroxol. Blood was then extracted from the patients to measure the levels of SP-A, interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor-α (TNF-α). The difference between group C and other groups in lung W/D and expression levels of SP-A mRNA and SP-A protein was significant (P<0.05). In comparison, the histological changes showed that the lung tissue damage was smallest in group C compared to the three other groups. Aspect of patients, 0.45% NaCl group and 0.9% NaCl+ambroxol group were significantly different in the levels of SP-A, IL-6, IL-8 and TNF-α (P<0.01). In the present study, 0.9% NaCl+ambroxol promote the synthesis and secretion of pulmonary surfactant, and has anti-inflammatory and antioxidant effects, which inhibit the release of inflammatory factors and cytokines, making it an ideal airway humidification liquid. PMID:26611525

  15. River basin administration

    NASA Astrophysics Data System (ADS)

    Management of international rivers and their basins is the focus of the Centre for Comparative Studies on (International) River Basin Administration, recently established at Delft University of Technology in the Netherlands. Water pollution, sludge, and conflicting interests in the use of water in upstream and downstream parts of a river basin will be addressed by studying groundwater and consumption of water in the whole catchment area of a river.Important aspects of river management are administrative and policy aspects. The Centre will focus on policy, law, planning, and organization, including transboundary cooperation, posing standards, integrated environmental planning on regional scale and environmental impact assessments.

  16. Tertiary Basins of Spain

    NASA Astrophysics Data System (ADS)

    Friend, Peter F.; Dabrio, Cristino J.

    1996-01-01

    During the Tertiary, Spain suffered compressional collision between France and Africa, and its Atlantic and Mediterranean coasts have been further modified by extensional rifting. Because it includes sectors of two separate foreland basins, and an intervening craton with basins that have been influenced by extensional and strikeSHslip deformation, Spain provides excellent material for the development and testing of theories on the study of sedimentary basin formation and filling. This book is one of the few studies available in English of the important Tertiary geology of Spain.

  17. K Basin Hazard Analysis

    SciTech Connect

    PECH, S.H.

    2000-08-23

    This report describes the methodology used in conducting the K Basins Hazard Analysis, which provides the foundation for the K Basins Final Safety Analysis Report. This hazard analysis was performed in accordance with guidance provided by DOE-STD-3009-94, Preparation Guide for U. S. Department of Energy Nonreactor Nuclear Facility Safety Analysis Reports and implements the requirements of DOE Order 5480.23, Nuclear Safety Analysis Report.

  18. K Basins Hazard Analysis

    SciTech Connect

    WEBB, R.H.

    1999-12-29

    This report describes the methodology used in conducting the K Basins Hazard Analysis, which provides the foundation for the K Basins Safety Analysis Report (HNF-SD-WM-SAR-062, Rev.4). This hazard analysis was performed in accordance with guidance provided by DOE-STD-3009-94, Preparation Guide for U. S. Department of Energy Nonreactor Nuclear Facility Safety Analysis Reports and implements the requirements of DOE Order 5480.23, Nuclear Safety Analysis Report.

  19. Can MLVA Differentiate among Endemic-Like MRSA Isolates with Identical Spa-Type in a Low-Prevalence Region?

    PubMed Central

    Blomfeldt, Anita; Hasan, Abdullahi Abdi; Aamot, Hege Vangstein

    2016-01-01

    The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Norway is low, but an endemic-like MRSA clone with Staphylococcal protein A (spa)-type t304 has been established especially in nursing homes in the Oslo region causing several large outbreaks. The challenge was that spa-typing and the gold standard Pulsed-Field Gel Electrophoresis (PFGE) were inadequate in discriminating isolates in outbreak investigations. Additional higher resolution genotyping methods were needed. The aims of this study were a) to evaluate whether Multiple-Locus Variable number of tandem repeat Analysis (MLVA) could differentiate within the PFGE clusters between epidemiologically related and unrelated endemic-like ST8-MRSA-IV-t304-PVL-neg (MRSA-t304) isolates and b) investigate the evolution of the endemic-like MRSA-t304 clone over a 15-year time period. All MRSA-t304 isolates detected in the region from 1998 through April 2013 were included. In total, 194 of 197 isolates were available for PFGE and MLVA analyses. PFGE results on isolates from 1998–2010 have been published previously. Two PFGE clusters subdivided into eight MLVA types were detected. One major outbreak clone (PFGE cluster C2/ MLVA type MT5045) appeared from 2004 to 2011 causing long-lasting and large outbreaks in seven nursing homes and one hospital. Five new MLVA types (N = 9 isolates) differing in only one VNTR compared to the outbreak clone C2/MT5045 were detected, but only one (C2/MT5044) was seen after 2011. We suggest that MLVA can replace PFGE analysis, but MLVA may not be the optimal method in this setting as it did not discriminate between all epidemiologically unrelated isolates. The results may indicate that all eight outbreaks in different locations within the PFGE C2 cluster may be branches of one large regional outbreak. The major outbreak strain C2/MT5045 may now, however, be under control, extinguished or has moved geographically. PMID:26859765

  20. Impact of sex and ozone exposure on the course of pneumonia in wild type and SP-A (−/−) mice

    PubMed Central

    Mikerov, Anatoly N.; Hu, Sanmei; Durrani, Faryal; Gan, Xiaozhuang; Wang, Guirong; Umstead, Todd M.; Phelps, David S.; Floros, Joanna

    2012-01-01

    Female mice exhibited higher survival rate than males after pneumonia, with a reversal of this pattern following ozone exposure. Surfactant protein A (SP-A) plays an important role in innate immunity and SP-A (−/−) mice were more susceptible to pneumonia than wild type mice. Here, we investigated underlying mechanisms of the differential susceptibility of mice to pneumonia. Wild type and SP-A (−/−) C57BL/6J male and female mice were exposed to ozone or filtered air (FA) and then infected intratracheally with Klebsiella pneumoniae. Blood, spleen, and lung were analyzed for bacterial counts, lung and spleen weights, and sex hormone and cortisol levels were measured in plasma within two days post-infection. We found: 1) in the absence of ozone-induced oxidative stress, males had higher level of bacterial dissemination compared to females; ozone exposure decreased pulmonary clearance in both sexes and ozone-exposed females were more affected than males; 2) ozone exposure increased lung weight, but decreased spleen weight in both sexes, and in both cases ozone-exposed females were affected the most; 3) plasma cortisol levels in infected mice changed: ozone-exposed > FA-exposed, females > males, and infected > non-infected; 4) no major sex hormone differences were observed in the studied conditions; 5) differences between wild type and SP-A (−/−) mice were observed in some of the studied conditions. We concluded that reduced pulmonary clearance, compromised spleen response to infection, and increased cortisol levels in ozone-exposed females, and the higher level of lung bacterial dissemination in FA-exposed males, contribute to the previously observed survival outcomes. PMID:22285567

  1. Co-brand strategy of evaluation of visual images in furniture design: Jimmy S.P.A. and STRAUSS as examples

    NASA Astrophysics Data System (ADS)

    Chen, Tien-Li; Pan, Fang-Ming; Tsai, Jen-Hui

    2013-03-01

    This study aimed to investigate the correlation of the image associated by the design Co-Brand (Jimmy S.P.A. and STRAUSS) and the impression perceived by subject of viewers. Visual images were used to examine the merit of the evaluation. The best result is provided using an object as an appropriate evaluation method. There are a lot of factors which influence to evaluation of a design. This study is limited to distinguish the appearance from Jimmy's picture books transform furniture and so on. Co-Brand of Jimmy S.P.A. and STRAUSS is not easy because there are not from the same cultural, and industry background and applying different marketing strategy, it is a way to combine the two brands by designing, used questionnaire of SD (Semantic differential evaluation) evaluation method to test out the perception of viewers, the objective of this study is to investigate and appraised the Co-Brands use by of the image in furniture from patrons. SD evaluation result showed, if design cannot understand the perception image of Jimmy S.P.A and STRAUSS with viewers mind, furniture design also can't transmit feeling with design.

  2. Genetic variant associations of human SP-A and SP-D with acute and chronic lung injury

    PubMed Central

    Silveyra, Patricia; Floros, Joanna

    2013-01-01

    Pulmonary surfactant, a lipoprotein complex, maintains alveolar integrity and plays an important role in lung host defense, and control of inflammation. Altered inflammatory processes and surfactant dysfunction are well described events that occur in patients with acute or chronic lung disease that can develop secondary to a variety of insults. Genetic variants of surfactant proteins, including single nucleotide polymorphisms, haplotypes, and other genetic variations have been associated with acute and chronic lung disease throughout life in several populations and study groups. The hydrophilic surfactant proteins SP-A and SP-D, also known as collectins, in addition to their surfactant-related functions, are important innate immunity molecules as these, among others, exhibit the ability to bind and enhance clearance of a wide range of pathogens and allergens. This review focuses on published association studies of human surfactant proteins A and D genetic polymorphisms with respiratory, and non-respiratory diseases in adults, children, and newborns. The potential role of genetic variations in pulmonary disease or pathogenesis is discussed following an evaluation, and comparison of the available literature. PMID:22201752

  3. Growth of NaCl on thin epitaxial KCl films on Ag(100) studied by SPA-LEED

    NASA Astrophysics Data System (ADS)

    Marquardt, Christian; Paulheim, Alexander; Sokolowski, Moritz

    2015-11-01

    We investigated the growth of NaCl on thin (100)-oriented films of KCl by spot profile analysis of low energy electron diffraction (SPA-LEED). The underlying question of this investigation was how the system accommodates to the misfit of - 10% between the NaCl and KCl lattices. The KCl films (3 atomic layers thick) were epitaxially grown on a Ag(100) single crystal. We studied the heteroepitaxial growth of NaCl on KCl at 300 K and at 500 K, respectively. At 300 K, the first NaCl monolayer (ML) grows pseudomorphically on the KCl film. From the second layer onward, the NaCl lattice relaxes. The NaCl multilayers roughen, and a small rotational disorder (± 4°) of the NaCl domains is observed. The roughening results from the formation of multilayer islands of limited lateral size due to the misfit to the pseudomorphic first NaCl layer. At a growth temperature of 500 K, no pseudomorphic NaCl layer forms, instead relaxed multilayer island growth of NaCl is observed from the first layer onward. Similarly to the growth at 300 K, we find NaCl multilayer islands of limited lateral size. For both temperatures, we explain this growth behavior by the misfit that makes the adsorption sites at the island edges of the first relaxed NaCl layer less favorable for larger islands, promoting nucleation of multilayer islands.

  4. [Hermann Lebert (1813-1878): natural scientist, spa doctor, histopathologist and clinician in Switzerland, France and Germany].

    PubMed

    Kaiser, H

    2010-07-01

    H. Lebert was in many ways an extraordinary personality. He began is career as a scientist performing experimental research in botany and zoology. After a short period as a spa doctor--work he approached on a scientific basis--he performed one of the first microscopic tissue analyses, as well as writing two significant works with a wealth of pictures on pathophysiology and histopathology, thereby paving the way for cellular pathology. In addition to general problems relating to inflammation, he concentrated on tumors and tuberculosis. He was one of the first to recommend pre-operative histology, making him a pioneer of biopsy diagnostics.As a clinician he worked in nearly all areas of internal medicine, including neurology, and published a large number of monographs, of which the first German monograph on acute articular rheumatism was one. Rheumatology played a considerable role in both his histopathological and clinical activities.Of particular interest is the fact that Lebert frequently travelled between Switzerland, France and Germany and was applauded in all three as a great scientist, as awards from both Napoleon III and the Prussian King Friedrich Wilhelm IV can testify.

  5. A system for identifying post-invasion functions of invasion genes: requirements for the Mxi-Spa type III secretion pathway of Shigella flexneri in intercellular dissemination.

    PubMed

    Schuch, R; Sandlin, R C; Maurelli, A T

    1999-11-01

    Invasion and intercellular spread are hallmarks of Shigella pathogenicity. Invasion of the eukaryotic cell cytosol requires a type III secretion system (Mxi-Spa) and its cognate set of secreted Ipa invasins. Once intracellular, the IcsA protein directs a form of actin-based motility that helps to drive intracellular bacterial movement, formation of cellular protrusions and cell-to-cell spread. Work in our laboratory has focused on identifying additional factors required for this intercellular form of dissemination. In this study, we sought to identify novel contributions of the type III secretion pathway to post-invasion-specific processes, distinct from its previously characterized roles in invasion. Studies of post-invasion Ipa and Mxi-Spa functions are complicated by an absolute requirement for these virulence proteins in invasion. To circumvent this problem, we developed a system called TIER (for test of intracellular expression requirements), whereby specific ipa, mxi or spa loci are transiently expressed before infection of tissue culture cell monolayers (thus supporting invasion), but then repressed after invasion in the intracellular environment. Such invasive type III secretion mutants (called TIER mutants) were severely restricted in their ability to spread intercellularly and form plaques in confluent tissue culture cell monolayers. Intercellular spread defects were associated with the repression of most type III pathway components examined, including structural (MxiM and Spa33), secreted effector (IpaB, IpaC and IpaD) and regulatory elements (VirF and VirB). A kinetic analysis of bacterial growth in L2 cell monolayers showed that each of the TIER mutants was defective with respect to long-term intracellular proliferation and viability. Examination of TIER mutant-infected monolayers by electron microscopy revealed that the type III pathway was required for a late step in intercellular spread - bacterial escape from protrusion-derived, double

  6. Nam Con Son Basin

    SciTech Connect

    Tin, N.T.; Ty, N.D.; Hung, L.T.

    1994-07-01

    The Nam Con Son basin is the largest oil and gas bearing basin in Vietnam, and has a number of producing fields. The history of studies in the basin can be divided into four periods: Pre-1975, 1976-1980, 1981-1989, and 1990-present. A number of oil companies have carried out geological and geophysical studies and conducted drilling activities in the basin. These include ONGC, Enterprise Oil, BP, Shell, Petro-Canada, IPL, Lasmo, etc. Pre-Tertiary formations comprise quartz diorites, granodiorites, and metamorphic rocks of Mesozoic age. Cenozoic rocks include those of the Cau Formation (Oligocene and older), Dua Formation (lower Miocene), Thong-Mang Cau Formation (middle Miocene), Nam Con Son Formation (upper Miocene) and Bien Dong Formation (Pliocene-Quaternary). The basement is composed of pre-Cenozoic formations. Three fault systems are evident in the basin: north-south fault system, northeast-southwest fault system, and east-west fault system. Four tectonic zones can also be distinguished: western differentiated zone, northern differentiated zone, Dua-Natuna high zone, and eastern trough zone.

  7. River basin management

    SciTech Connect

    Newsome, D.H.; Edwards, A.M.C.

    1984-01-01

    The quality of water is of paramount importance in the management of water resources - including marine waters. A quantitative knowledge of water quality and the factors governing it is required to formulate and implement strategies requiring an inter-disciplinary approach. The overall purpose of this conference was to bring together the latest work on water quality aspects of river basin management. These proceedings are structured on the basis of five themes: problems in international river basins; the contribution of river systems to estuarial and marine pollution; the setting of standards; monitoring; and practical water quality management including use of mathematical models. They are followed by papers from the workshop on advances in the application of mathematical modelling to water quality management, which represent some of the current thinking on the problems and concepts of river basin management.

  8. Delaware River Basin

    USGS Publications Warehouse

    Fischer, Jeffrey M.

    1999-01-01

    Assessing the quality of water in every location of the Nation would not be practical. Therefore, NAWQA investigations are conducted within 59 selected areas called study units (fig. 1). These study units encompass important river and aquifer systems in the United States and represent the diverse geographic, waterresource, land-use, and water-use characteristics of the Nation. The Delaware River Basin is one of 15 study units in which work began in 1996. Water-quality sampling in the study unit will begin in 1999. This fact sheet provides a brief overview of the NAWQA program, describes the Delaware River Basin study unit, identifies the major water-quality issues in the basin, and documents the plan of study that will be followed during the study-unit investigation.

  9. Geology, exploration status of Uruguay's sedimentary basins

    SciTech Connect

    Goso, C.; Santa Ana, H. de )

    1994-02-07

    This article attempts to present the geological characteristics and tectonic and sedimentary evolution of Uruguayan basins and the extent to which they have been explored. Uruguay is on the Atlantic coast of South America. The country covers about 318,000 sq km, including offshore and onshore territories corresponding to more than 65% of the various sedimentary basins. Four basins underlie the country: the Norte basin, the Santa Lucia basin, the offshore Punta del Este basin, and the offshore-onshore Pelotas-Merin basin. The Norte basin is a Paleozoic basin while the others are Mesozoic basins. Each basin has been explored to a different extent, as this paper explains.

  10. Trinity river basin, Texas

    USGS Publications Warehouse

    Ulery, Randy L.; Van Metre, Peter C.; Crossfield, Allison S.

    1993-01-01

    In 1991 the Trinity River Basin National Water-Quality Assessment (NAWQA) will include assessments of surface-water and ground-water quality. Initial efforts have focused on identifying water-quality issues in the basin and on the environmental factors underlying those issues. Physical characteristics described include climate, geology, soils, vegetation, physiography, and hydrology. Cultural characteristics discussed include population distribution, land use and land cover, agricultural practices, water use, an reservoir operations. Major water-quality categories are identified and some of the implications of the environmental factors for water quality are presented.

  11. Taunton River basin

    USGS Publications Warehouse

    Williams, John R.; Willey, Richard E.

    1970-01-01

    This report presents in tabular form selected records of wells, test wells, and borings collected during a study of the basin from 1966 to 1968 in cooperation with the Massachusetts Water Resources Commission, and during earlier studies. This report is released in order to make available to the public and to local, state, and federal agencies basic ground-water information that may aid in planning water-resources development. Basic records contained in this report will complement an interpretative report on the Taunton River basin to be released at a later date.

  12. Staphylococcus aureus spa type t437: identification of the most dominant community-associated clone from Asia across Europe.

    PubMed

    Glasner, C; Pluister, G; Westh, H; Arends, J P; Empel, J; Giles, E; Laurent, F; Layer, F; Marstein, L; Matussek, A; Mellmann, A; Pérez-Vásquez, M; Ungvári, E; Yan, X; Žemličková, H; Grundmann, H; van Dijl, J M

    2015-02-01

    Methicillin-resistant Staphylococcus aureus (MRSA) belonging to the multilocus sequence type clonal complex 59 (MLST CC59) is the predominant community-associated MRSA clone in Asia. This clone, which is primarily linked with the spa type t437, has so far only been reported in low numbers among large epidemiological studies in Europe. Nevertheless, the overall numbers identified in some Northern European reference laboratories have increased during the past decade. To determine whether the S. aureus t437 clone is present in other European countries, and to assess its genetic diversity across Europe, we analysed 147 S. aureus t437 isolates from 11 European countries collected over a period of 11 years using multiple locus variable number tandem repeat fingerprinting/analysis (MLVF/MLVA) and MLST. Additionally 16 S. aureus t437 isolates from healthy carriers and patients from China were included. Most isolates were shown to be monophyletic with 98% of the isolates belonging to the single MLVA complex 621, to which nearly all included isolates from China also belonged. More importantly, all MLST-typed isolates belonged to CC59. Our study implies that the European S. aureus t437 population represents a genetically tight cluster, irrespective of the year, country and site of isolation. This underpins the view that S. aureus CC59 has been introduced into several European countries, not being restricted to particular geographical regions or specific host environments. The European S. aureus t437 isolates thus bear the general hallmarks of a high-risk clone. PMID:25658555

  13. Radon Levels Measured at a Touristic Thermal Spa Resort in Montagu (South Africa) and Associated Effective Doses.

    PubMed

    Botha, R; Newman, R T; Maleka, P P

    2016-09-01

    Radon activity concentrations (in water and in air) were measured at 13 selected locations at the Avalon Springs thermal spa resort in Montagu (Western Cape, South Africa) to estimate the associated effective dose received by employees and visitors. A RAD-7 detector (DURRIDGE), based on alpha spectrometry, and electret detectors (E-PERM®Radelec) were used for these radon measurements. The primary source of radon was natural thermal waters from the hot spring, which were pumped to various locations on the resort, and consequently a range of radon in-water analyses were performed. Radon in-water activity concentration as a function of time (short term and long term measurements) and spatial distributions (different bathing pools, etc.) were studied. The mean radon in-water activity concentrations were found to be 205 ± 6 Bq L (source), 112 ± 5 Bq L (outdoor pool) and 79 ± 4 Bq L (indoor pool). Radon in-air activity concentrations were found to range between 33 ± 4 Bq m (at the outside bar) to 523 ± 26 Bq m (building enclosing the hot spring's source). The most significant potential radiation exposure identified is that due to inhalation of air rich in radon and its progeny by the resort employees. The annual occupational effective dose due to the inhalation of radon progeny ranges from 0.16 ± 0.01 mSv to 0.40 ± 0.02 mSv. For the water samples collected, the Ra in-water activity concentrations from samples collected were below the lower detection limit (~0.7 Bq L) of the γ-ray detector system used. No significant radiological health risk can be associated with radon and progeny from the hot spring at the Avalon Springs resort. PMID:27472753

  14. The detailed analysis of natural radionuclides dissolved in spa waters of the Kłodzko Valley, Sudety Mountains, Poland.

    PubMed

    Walencik-Łata, A; Kozłowska, B; Dorda, J; Przylibski, T A

    2016-11-01

    A survey was conducted to measure natural radioactivity in spa waters from the Kłodzko Valley. The main goal of this study was to determine the activity concentration of uranium, radium and radon isotopes in the investigated groundwaters. Samples were collected several times from 35 water intakes from 5 spas and 2 mineral water bottling plants. The authors examined whether the increased gamma radiation background, as well as the elevated values of radium and uranium content in reservoir rocks, have a significant impact on the natural radioactivity of these waters. The second objective of this research was to provide information about geochemistry of U, Ra, Rn radionuclides and the radiological and chemical risks incurred by ingestion of isotopes with drinking water. On the basis of results obtained, it is feasible to assess the health hazard posed by ingestion of natural radioactivity with drinking waters. Moreover, the data yielded by this research may be helpful in the process of verification of the application of these waters in balneotherapy. In addition, annual effective radiation doses resulting from the isotopes consumption were calculated on the basis of the evaluated activity concentrations. In dose assessment for uranium and radium isotopes, the authors provided values for different human age groups. The obtained uranium content in the investigated waters was compared with the currently valid regulations concerning the quality of drinking water. Based on the activity concentrations data, the activity isotopic ratios (234)U/(238)U, (226)Ra/(238)U, (222)Rn/(238)U, (222)Rn/(226)Ra and the correlations between radionuclides content were then examined. In brief, it may be concluded on the basis of the obtained results that radon solubility is inversely proportional to radium and uranium dissolution in environmental water circulation. The presented study allows conclusions to be drawn on the radionuclide circulation among different environmental biota: from

  15. Uranium and thorium behavior in groundwater of the natural spa area “Choygan mineral water” (East Tuva)

    NASA Astrophysics Data System (ADS)

    Kopylova, Y.; Guseva, N.; Shestakova, A.; Khvaschevskaya, A.; Arakchaa, K.

    2015-11-01

    The natural spa area “Choygan mineral waters”, a unique deposit of natural carbon dioxide mineral waters in Siberia, is located in the Eastern Sayan Mountains. There are 33 spring discharges in this area. Spring waters are mainly of HCO3-Na-Ca type. TDS varies from 300 mg/L to 2600 mg/L, the temperature ranges from 7 °C (in spring 33) to 39 °C (in spring 12), pH varies from 5.9 to 8.3, and the value of the oxidation-reduction potential is from -170 mV to 236 mV. All studied waters were divided into two groups according to their temperature and geochemical conditions: cold fresh water in oxidizing environment and warm slightly brackish water in reducing environment. The uranium concentration varies from 0.7 to 14 μg/l and the thorium concentration varies from 0.001 to 0.33 μg/l in the studied waters. The predominant uranium complexes are (UO2(CO3)3)4-, (UO2(CO3)2)2-, UO2CO3, (UO2(PO4)2)4- in the waters in oxidizing and reducing environments. It was found that acid-alkaline and oxidizing-reducing conditions were the determining factors for uranium behavior and speciation in the studied waters. The pH conditions are determining factors for thorium behavior and speciation in the studied waters. In slightly acidic water the predominant thorium species is negatively charge complex (ThCO3(OH)3)- (more than 95%).

  16. The detailed analysis of natural radionuclides dissolved in spa waters of the Kłodzko Valley, Sudety Mountains, Poland.

    PubMed

    Walencik-Łata, A; Kozłowska, B; Dorda, J; Przylibski, T A

    2016-11-01

    A survey was conducted to measure natural radioactivity in spa waters from the Kłodzko Valley. The main goal of this study was to determine the activity concentration of uranium, radium and radon isotopes in the investigated groundwaters. Samples were collected several times from 35 water intakes from 5 spas and 2 mineral water bottling plants. The authors examined whether the increased gamma radiation background, as well as the elevated values of radium and uranium content in reservoir rocks, have a significant impact on the natural radioactivity of these waters. The second objective of this research was to provide information about geochemistry of U, Ra, Rn radionuclides and the radiological and chemical risks incurred by ingestion of isotopes with drinking water. On the basis of results obtained, it is feasible to assess the health hazard posed by ingestion of natural radioactivity with drinking waters. Moreover, the data yielded by this research may be helpful in the process of verification of the application of these waters in balneotherapy. In addition, annual effective radiation doses resulting from the isotopes consumption were calculated on the basis of the evaluated activity concentrations. In dose assessment for uranium and radium isotopes, the authors provided values for different human age groups. The obtained uranium content in the investigated waters was compared with the currently valid regulations concerning the quality of drinking water. Based on the activity concentrations data, the activity isotopic ratios (234)U/(238)U, (226)Ra/(238)U, (222)Rn/(238)U, (222)Rn/(226)Ra and the correlations between radionuclides content were then examined. In brief, it may be concluded on the basis of the obtained results that radon solubility is inversely proportional to radium and uranium dissolution in environmental water circulation. The presented study allows conclusions to be drawn on the radionuclide circulation among different environmental biota: from

  17. Sedimentary basins and crustal thickening

    NASA Astrophysics Data System (ADS)

    Cobbold, P. R.; Davy, P.; Gapais, D.; Rossello, E. A.; Sadybakasov, E.; Thomas, J. C.; Tondji Biyo, J. J.; de Urreiztieta, M.

    1993-07-01

    We consider the development of sedimentary basins in a tectonic context dominated by horizontal shortening and vertical thickening of the crust. Well-known examples are foreland basins; others are ramp basins and buckle basins. We have reproduced various styles of compressional basins in experiments, properly scaled for gravity. A multilayered model lithosphere, with brittle and ductile layers, floats on a model asthenosphere. A computer-driven piston provides shortening and thickening, synchronous with erosion and sedimentation. After a first stage of lithospheric buckling, thrust faults appear, mainly at inflection points. Slip on an isolated reverse fault is accompanied by flexure. Footwall flexure results in a foreland basin and becomes accentuated by sedimentation. Hangingwall flexure is less marked, but may become accentuated by erosion. Motion on a fault leads to hangingwall collapse at the surface. Either footwall sedimentation or hangingwall erosion tends to prolong the active life of a reverse fault. Slip on any pair of closely spaced reverse faults of opposite vergence results in a ramp basin. Simultaneous slip produces a symmetric ramp basin, whereas alternating slip results in a butterfly-shaped basin, with superposed foredeeps. Some well-developed ramp basins become pushed down, until bounding faults meet at the surface and the basin disappears from view. At this stage, the basin depth is equivalent to 15 km or more. Slip on any pair of widely spaced reverse faults of opposite vergence results in a pronounced central anticline, between two distinct foredeeps. In Central Asia and in Western Europe, Cenozoic crustal thickening is due to continental collision. For Central Asia (Western China, Kyrgyzstan, Uzbekistan, Tajikistan), we have compiled a regional structure-contour map on the base of the Tertiary, as well as 4 regional sections. Foreland basins and ramp basins are numerous and associated with Cenozoic thrusts. Large basins (Tarim, Junggar

  18. Lunar Meteorites Sayh Al Uhaymir 449 and Dhofar 925, 960, and 961: Windows into South Pole

    NASA Technical Reports Server (NTRS)

    Ziegler, Ryan A.; Jolliff, B. L.; Korotev, R. L.

    2013-01-01

    In 2003, three lunar meteorites were collected in close proximity to each other in the Dhofar region of Oman: Dhofar 925 (49 g), Dhofar 960 (35 g), and Dhofar 961 (22 g). In 2006, lunar meteorite Sayh al Uhaymir (SaU) 449 (16.5 g) was found about 100 km to the NE. Despite significant differences in the bulk composition of Dhofar 961 relative to Dhofar 925/960 and SaU 449 (which are identical to each other), these four meteorites are postulated to be paired based on their find locations, bulk composition, and detailed petrographic analysis. Hereafter, they will collectively be referred to as the Dhofar 961 clan. Comparison of meteorite and component bulk compositions to Lunar Prospector 5-degree gamma-ray data suggest the most likely provenance of this meteorite group is within the South Pole-Aitken Basin. As the oldest, largest, and deepest recognizable basin on the Moon, the composition of the material within the SPA basin is of particular importance to lunar science. Here we review and expand upon the geochemistry and petrography of the Dhofar 961 clan and assess the likelihood that these meteorites come from within the SPA basin based on their bulk compositions and the compositions and characteristics of the major lithologic components found within the breccia.

  19. Cenozoic basin development in Hispaniola

    SciTech Connect

    Mann, P.; Burke, K.

    1984-04-01

    Four distinct generations of Cenozoic basins have developed in Hispaniola (Haiti and Dominican Republic) as a result of collisional or strike-slip interactions between the North America and Caribbean plates. First generation basins formed when the north-facing Hispaniola arc collided with the Bahama platform in the middle Eocene; because of large post-Eocene vertical movements, these basins are preserved locally in widely separated areas but contain several kilometers of arc and ophiolite-derived clastic marine sediments, probably deposited in thrust-loaded, flexure-type basins. Second generation basins, of which only one is exposed at the surface, formed during west-northwesterly strike-slip displacement of southern Cuba and northern Hispaniola relative to central Hispaniola during the middle to late Oligocene; deposition occurred along a 5-km (3-mi) wide fault-angle depression and consisted of about 2 km (1 mi) of submarine fan deposits. Third generation basins developed during post-Oligocene convergent strike-slip displacement across a restraining bend formed in central Hispaniola; the southern 2 basins are fairly symmetrical, thrust-bounded ramp valleys, and the third is an asymmetrical fault-angle basin. Fourth generation basins are pull-aparts formed during post-Miocene divergent strike-slip motion along a fault zone across southern Hispaniola. As in other Caribbean areas, good source rocks are present in all generations of basins, but suitable reservoir rocks are scarce. Proven reservoirs are late Neogene shallow marine and fluvial sandstones in third generation basins.

  20. Clinical and biochemical effects of a 3-week program of diet combined with spa therapy in obese and diabetic patients: a pilot open study

    NASA Astrophysics Data System (ADS)

    Fioravanti, Antonella; Adamczyk, Przemysław; Pascarelli, Nicola Antonio; Giannitti, Chiara; Urso, Renato; Tołodziecki, Michał; Ponikowska, Irena

    2015-07-01

    Obesity is a major risk factor for arterial hypertension, coronary artery disease, dyslipidemias, and type 2 diabetes. Spa therapy has long been used for treating obesity and its comorbidities. Enlargement of adipose tissue has been linked to a dysregulation of adipokine secretion and adipose tissue inflammation. Adipokines are currently investigated as potential drug targets in these conditions. Our primary aim was to assess the clinical efficacy of a 3-week program of diet combined with spa therapy in obese patients with and without type 2 diabetes. The secondary aim was to examine whether this combined program influences the response of serum levels of leptin, adiponectin, visfatin, and high-sensitivity C-reactive protein. Fifty obese males were enrolled and 21 of these featured a type 2 diabetes. During the 3-week period of the study, the patients were on a 1,000-kcal diet and were involved in mineral bath and total body's mud-pack applications (15 procedures). Patients were assessed at baseline and at the end of the therapy for clinical and biochemical parameters (total cholesterol, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, glycemia, and adipokines). We showed that a 3-week program of spa therapy in obese patients induced significant decrease of body weight, body mass index, triglycerides, total cholesterol, low-density lipoprotein (LDL) cholesterol, glycemia, and serum levels of leptin and high-sensitivity C-reactive protein. So, a cycle of mud-bath therapy associated with a controlled diet may be a promising treatment for obesity and type 2 diabetes decreasing body weight and many risk factors for atherosclerosis and metabolic syndrome.

  1. A slow-forming isopeptide bond in the structure of the major pilin SpaD from Corynebacterium diphtheriae has implications for pilus assembly

    SciTech Connect

    Kang, Hae Joo; Paterson, Neil G.; Kim, Chae Un; Middleditch, Martin; Chang, Chungyu; Ton-That, Hung; Baker, Edward N.

    2014-05-01

    Two crystal structures of the major pilin SpaD from C. diphtheriae have been determined at 1.87 and 2.5 Å resolution. The N-terminal domain is found to contain an isopeptide bond that forms slowly over time in the recombinant protein. Given its structural context, this provides insight into the relationship between internal isopeptide-bond formation and pilus assembly. The Gram-positive organism Corynebacterium diphtheriae, the cause of diphtheria in humans, expresses pili on its surface which it uses for adhesion and colonization of its host. These pili are covalent protein polymers composed of three types of pilin subunit that are assembled by specific sortase enzymes. A structural analysis of the major pilin SpaD, which forms the polymeric backbone of one of the three types of pilus expressed by C. diphtheriae, is reported. Mass-spectral and crystallographic analysis shows that SpaD contains three internal Lys–Asn isopeptide bonds. One of these, shown by mass spectrometry to be located in the N-terminal D1 domain of the protein, only forms slowly, implying an energy barrier to bond formation. Two crystal structures, of the full-length three-domain protein at 2.5 Å resolution and of a two-domain (D2-D3) construct at 1.87 Å resolution, show that each of the three Ig-like domains contains a single Lys–Asn isopeptide-bond cross-link, assumed to give mechanical stability as in other such pili. Additional stabilizing features include a disulfide bond in the D3 domain and a calcium-binding loop in D2. The N-terminal D1 domain is more flexible than the others and, by analogy with other major pilins of this type, the slow formation of its isopeptide bond can be attributed to its location adjacent to the lysine used in sortase-mediated polymerization during pilus assembly.

  2. Long-term benefits of radon spa therapy in rheumatic diseases: results of the randomised, multi-centre IMuRa trial.

    PubMed

    Annegret, Franke; Thomas, Franke

    2013-11-01

    In chronic rheumatic diseases, recent treatment regimens comprise multimodal concepts including pharmacologic, physical/exercise, occupational and psychological therapies. Rehabilitation programmes are used for long-term management of disease. Spa therapy is often integrated in various middle and south European and Asian countries. Here, we investigated radon spa therapy as applied in health resorts compared to a control intervention in rheumatic out-patients. Randomised, blinded trial enroling 681 patients [mean age 58.3 (standard deviation 11.1); female 59.7%] in 7 health resorts in Germany and Austria with chronic back pain (n 1 = 437), osteoarthritis (OA) (n 2 = 230), rheumatoid arthritis (n 3 = 98), and/or ankylosing spondylitis (n 4 = 39); multiple nominations in 146 cases). Outcomes were pain (primary), quality of life, functional capacity, and medication measured before start, after end of treatment, and 3 times thereafter in 3 monthly intervals. Adverse events were documented. To analyse between-group differences, repeated-measures analysis of covariance was performed in metric endpoints and Fisher's exact test in rates. Two-sided significance level of 5% was chosen. Until end of follow-up, superiority of radon therapy was found regarding pain relief (p = 0.032) and analgesic drug consumption (p = 0.007), but not regarding quality of life. Functional capacity was assessed specific to the underlying indication. Significant benefits were found in radon-treated OA patients until 6-month follow-up (p = 0.05), but not until end of study (p = 0.096). Neither the back pain sub-population nor the two smaller patient populations with inflammatory indications benefited significantly in functional capacity. Results suggest beneficial analgesic effects of radon spa therapy in rheumatic diseases until 9 months post-intervention.

  3. Natural frequency of regular basins

    NASA Astrophysics Data System (ADS)

    Tjandra, Sugih S.; Pudjaprasetya, S. R.

    2014-03-01

    Similar to the vibration of a guitar string or an elastic membrane, water waves in an enclosed basin undergo standing oscillatory waves, also known as seiches. The resonant (eigen) periods of seiches are determined by water depth and geometry of the basin. For regular basins, explicit formulas are available. Resonance occurs when the dominant frequency of external force matches the eigen frequency of the basin. In this paper, we implement the conservative finite volume scheme to 2D shallow water equation to simulate resonance in closed basins. Further, we would like to use this scheme and utilizing energy spectra of the recorded signal to extract resonant periods of arbitrary basins. But here we first test the procedure for getting resonant periods of a square closed basin. The numerical resonant periods that we obtain are comparable with those from analytical formulas.

  4. Buried-euxenic-basin model sets Tarim basin potential

    SciTech Connect

    Hsu, K.J. )

    1994-11-28

    The Tarim basin is the largest of the three large sedimentary basins of Northwest China. The North and Southwest depressions of Tarim are underlain by thick sediments and very thin crust. The maximum sediment thickness is more than 15 km. Of the several oil fields of Tarim, the three major fields were discovered during the last decade, on the north flank of the North depression and on the Central Tarim Uplift. The major targets of Tarim, according to the buried-euxenic-basin model, should be upper Paleozoic and lower Mesozoic reservoirs trapping oil and gas condensates from lower Paleozoic source beds. The paper describes the basin and gives a historical perspective of exploration activities and discoveries. It then explains how this basin can be interpreted by the buried-euxenic-basin model. The buried-euxenic-basin model postulates four stages of geologic evolution: (1) Sinian and early Paleozoic platform sedimentation on relic arcs and deep-marine sedimentation in back-arc basins in Xinjiang; (2) Late Paleozoic foreland-basin sedimentation in north Tarim; (3) Mesozoic and Paleogene continental deposition, subsidence under sedimentary load; and (4) Neogene pull-apart basin, wrench faulting and extension.

  5. Canada Basin revealed

    USGS Publications Warehouse

    Mosher, David C.; Shimeld, John; Hutchinson, Deborah R.; Chian, D; Lebedeva-Ivanova, Nina; Jackson, Ruth

    2012-01-01

    More than 15,000 line-km of new regional seismic reflection and refraction data in the western Arctic Ocean provide insights into the tectonic and sedimentologic history of Canada Basin, permitting development of new geologic understanding in one of Earth's last frontiers. These new data support a rotational opening model for southern Canada Basin. There is a central basement ridge possibly representing an extinct spreading center with oceanic crustal velocities and blocky basement morphology characteristic of spreading centre crust surrounding this ridge. Basement elevation is lower in the south, mostly due to sediment loading subsidence. The sedimentary succession is thickest in the southern Beaufort Sea region, reaching more than 15 km, and generally thins to the north and west. In the north, grabens and half-grabens are indicative of extension. Alpha-Mendeleev Ridge is a large igneous province in northern Amerasia Basin, presumably emplaced synchronously with basin formation. It overprints most of northern Canada Basin structure. The seafloor and sedimentary succession of Canada Basin is remarkably flat-lying in its central region, with little bathymetric change over most of its extent. Reflections that correlate over 100s of kms comprise most of the succession and on-lap bathymetric and basement highs. They are interpreted as representing deposits from unconfined turbidity current flows. Sediment distribution patterns reflect changing source directions during the basin’s history. Initially, probably late Cretaceous to Paleocene synrift sediments sourced from the Alaska and Mackenzie-Beaufort margins. This unit shows a progressive series of onlap unconformities with a younging trend towards Alpha and Northwind ridges, likely a response to contemporaneous subsidence. Sediment source direction appeared to shift to the Canadian Arctic Archipelago margin for the Eocene and Oligocene, likely due to uplift of Arctic islands during the Eurekan Orogeny. The final

  6. The Use of Terrestrial Analogs in Preparing for Planetary Surface Exploration: Sampling and Radioisotopic Dating of Impactites and Deployment of In Situ Analytical Technologies

    NASA Astrophysics Data System (ADS)

    Young, Kelsey

    Impact cratering has played a crucial role in the surface development of the inner planets. Constraining the timing of this bombardment history is important in understanding the origins of life and our planet's evolution. Plate tectonics, active volcanism, and vegetation hinder the preservation and identification of existing impact craters on Earth. Providing age constraints on these elusive structures will provide a deeper understanding of our planet's development. To do this, (U-Th)/He thermochronology and in situ 40 Ar/39Ar laser microprobe geochronology are used to provide ages for the Haughton and Mistastin Lake impact structures, both located in northern Canada. While terrestrial impact structures provide accessible laboratories for deciphering Earth's impact history, the ultimate goal for understanding the history of the reachable inner Solar System is to acquire robust, quantitative age constraints for the large lunar impact basins. The oldest of these is the South Pole-Aitken basin (SPA), located on the lunar farside. While it is known that this basin is stratigraphically the oldest on the Moon, its absolute age has yet to be determined. Several reports released in the last decade have highlighted sampling and dating SPA as a top priority for inner Solar System exploration. This is no easy task as the SPA structure has been modified by four billion subsequent years of impact events. Informed by studies at Mistastin---which has target lithologies analogous to those at SPA---sampling strategies are discussed that are designed to optimize the probability of a high science return with regard to robust geochronology of the SPA basin. Planetary surface missions, like one designed to explore and sample SPA, require the integration of engineering constraints with scientific goals and traverse planning. The inclusion of in situ geochemical technology, such as the handheld X-ray fluorescence spectrometer (hXRF), into these missions will provide human crews with the

  7. Albuquerque Basin seismic network

    USGS Publications Warehouse

    Jaksha, Lawrence H.; Locke, Jerry; Thompson, J.B.; Garcia, Alvin

    1977-01-01

    The U.S. Geological Survey has recently completed the installation of a seismic network around the Albuquerque Basin in New Mexico. The network consists of two seismometer arrays, a thirteen-station array monitoring an area of approximately 28,000 km 2 and an eight-element array monitoring the area immediately adjacent to the Albuquerque Seismological Laboratory. This report describes the instrumentation deployed in the network.

  8. Petroleum basin studies

    SciTech Connect

    Shannon, P.M. ); Naylor, D. )

    1989-01-01

    This book reviews the tectonic setting, basin development and history of exploration of a number of selected petroleum provinces located in a variety of settings in the Middle East, North Sea, Nigeria, the Rocky Mountains, Gabon and China. This book illustrates how ideas and models developed in one area may be applied to other regions. Regional reviews and the reassessment of petroleum provinces are presented.

  9. Dimension of fractal basin boundaries

    SciTech Connect

    Park, B.S.

    1988-01-01

    In many dynamical systems, multiple attractors coexist for certain parameter ranges. The set of initial conditions that asymptotically approach each attractor is its basin of attraction. These basins can be intertwined on arbitrary small scales. Basin boundary can be either smooth or fractal. Dynamical systems that have fractal basin boundary show final state sensitivity of the initial conditions. A measure of this sensitivity (uncertainty exponent {alpha}) is related to the dimension of the basin boundary d = D - {alpha}, where D is the dimension of the phase space and d is the dimension of the basin boundary. At metamorphosis values of the parameter, there might happen a conversion from smooth to fractal basin boundary (smooth-fractal metamorphosis) or a conversion from fractal to another fractal basin boundary characteristically different from the previous fractal one (fractal-fractal metamorphosis). The dimension changes continuously with the parameter except at the metamorphosis values where the dimension of the basin boundary jumps discontinuously. We chose the Henon map and the forced damped pendulum to investigate this. Scaling of the basin volumes near the metamorphosis values of the parameter is also being studied for the Henon map. Observations are explained analytically by using low dimensional model map.

  10. Light and the E3 ubiquitin ligase COP1/SPA control the protein stability of the MYB transcription factors PAP1 and PAP2 involved in anthocyanin accumulation in Arabidopsis.

    PubMed

    Maier, Alexander; Schrader, Andrea; Kokkelink, Leonie; Falke, Christian; Welter, Bastian; Iniesto, Elisa; Rubio, Vicente; Uhrig, Joachim F; Hülskamp, Martin; Hoecker, Ute

    2013-05-01

    Anthocyanins are natural pigments that accumulate only in light-grown and not in dark-grown Arabidopsis plants. Repression of anthocyanin accumulation in darkness requires the CONSTITUTIVELY PHOTOMORPHOGENIC1/SUPPRESSOR OF PHYA-105 (COP1/SPA) ubiquitin ligase, as cop1 and spa mutants produce anthocyanins also in the dark. Here, we show that COP1 and SPA proteins interact with the myeloblastosis (MYB) transcription factors PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP)1 and PAP2, two members of a small protein family that is required for anthocyanin accumulation and for the expression of structural genes in the anthocyanin biosynthesis pathway. The increased anthocyanin levels in cop1 mutants requires the PAP1 gene family, indicating that COP1 functions upstream of the PAP1 gene family. PAP1 and PAP2 proteins are degraded in the dark and this degradation is dependent on the proteasome and on COP1. Hence, the light requirement for anthocyanin biosynthesis results, at least in part, from the light-mediated stabilization of PAP1 and PAP2. Consistent with this conclusion, moderate overexpression of PAP1 leads to an increase in anthocyanin levels only in the light and not in darkness. Here we show that SPA genes are also required for reducing PAP1 and PAP2 transcript levels in dark-grown seedlings. Taken together, these results indicate that the COP1/SPA complex affects PAP1 and PAP2 both transcriptionally and post-translationally. Thus, our findings have identified mechanisms via which the COP1/SPA complex controls anthocyanin levels in Arabidopsis that may be useful for applications in biotechnology directed towards increasing anthocyanin content in plants.

  11. Great Basin Paleontological Bibliography

    USGS Publications Warehouse

    Blodgett, Robert B.; Zhang, Ning; Hofstra, Albert H.; Morrow, Jared R.

    2007-01-01

    Introduction This work was conceived as a derivative product for 'The Metallogeny of the Great Basin' project of the Mineral Resources Program of the U.S. Geological Survey. In the course of preparing a fossil database for the Great Basin that could be accessed from the Internet, it was determined that a comprehensive paleontological bibliography must first be compiled, something that had not previously been done. This bibliography includes published papers and abstracts as well as unpublished theses and dissertations on fossils and stratigraphy in Nevada and adjoining portions of California and Utah. This bibliography is broken into first-order headings by geologic age, secondary headings by taxonomic group, followed by ancillary topics of interest to both paleontologists and stratigraphers; paleoecology, stratigraphy, sedimentary petrology, paleogeography, tectonics, and petroleum potential. References were derived from usage of Georef, consultation with numerous paleontologists and geologists working in the Great Basin, and literature currently on hand with the authors. As this is a Web-accessible bibliography, we hope to periodically update it with new citations or older references that we have missed during this compilation. Hence, the authors would be grateful to receive notice of any new or old papers that the readers think should be added. As a final note, we gratefully acknowledge the helpful reviews provided by A. Elizabeth J. Crafford (Anchorage, Alaska) and William R. Page (USGS, Denver, Colorado).

  12. Advanced Chemistry Basins Model

    SciTech Connect

    William Goddard; Mario Blanco; Lawrence Cathles; Paul Manhardt; Peter Meulbroek; Yongchun Tang

    2002-11-10

    The DOE-funded Advanced Chemistry Basin model project is intended to develop a public domain, user-friendly basin modeling software under PC or low end workstation environment that predicts hydrocarbon generation, expulsion, migration and chemistry. The main features of the software are that it will: (1) afford users the most flexible way to choose or enter kinetic parameters for different maturity indicators; (2) afford users the most flexible way to choose or enter compositional kinetic parameters to predict hydrocarbon composition (e.g., gas/oil ratio (GOR), wax content, API gravity, etc.) at different kerogen maturities; (3) calculate the chemistry, fluxes and physical properties of all hydrocarbon phases (gas, liquid and solid) along the primary and secondary migration pathways of the basin and predict the location and intensity of phase fractionation, mixing, gas washing, etc.; and (4) predict the location and intensity of de-asphaltene processes. The project has be operative for 36 months, and is on schedule for a successful completion at the end of FY 2003.

  13. Caribbean basin framework, 3: Southern Central America and Colombian basin

    SciTech Connect

    Kolarsky, R.A.; Mann, P. )

    1991-03-01

    The authors recognize three basin-forming periods in southern Central America (Panama, Costa Rica, southern Nicaragua) that they attempt to correlate with events in the Colombian basin (Bowland, 1984): (1) Early-Late Cretaceous island arc formation and growth of the Central American island arc and Late Cretaceous formation of the Colombian basin oceanic plateau. During latest Cretaceous time, pelagic carbonate sediments blanketed the Central American island arc in Panama and Costa Rica and elevated blocks on the Colombian basin oceanic plateau; (2) middle Eocene-middle Miocene island arc uplift and erosion. During this interval, influx of distal terrigenous turbidites in most areas of Panama, Costa Rica, and the Colombian basin marks the uplift and erosion of the Central American island arc. In the Colombian basin, turbidites fill in basement relief and accumulate to thicknesses up to 2 km in the deepest part of the basin. In Costa Rica, sedimentation was concentrated in fore-arc (Terraba) and back-arc (El Limon) basins; (3) late Miocene-Recent accelerated uplift and erosion of segments of the Central American arc. Influx of proximal terrigenous turbidites and alluvial fans in most areas of Panama, Costa Rica, and the Colombian basin marks collision of the Panama arc with the South American continent (late Miocene early Pliocene) and collision of the Cocos Ridge with the Costa Rican arc (late Pleistocene). The Cocos Ridge collision inverted the Terraba and El Limon basins. The Panama arc collision produced northeast-striking left-lateral strike-slip faults and fault-related basins throughout Panama as Panama moved northwest over the Colombian basin.

  14. A combined STM and SPA-LEED study of the “explosive” nucleation and collective diffusion in Pb/Si(111)

    DOE PAGES

    Hattab, H.; Hupalo, M.; Hershberger, M. T.; Horn von Hoegen, M.; Tringides, M. C.

    2015-08-20

    A novel type of very fast nucleation was recently found in Pb/Si(111) with 4- to 7-layer high islands becoming crystalline in an “explosive” way, when the Pb deposited amount in the wetting layer is compressed to θc ~ 1.22 ML, well above the metallic Pb(111) density. This “explosive” nucleation is very different from classical nucleation when island growth is more gradual and islands grow in size by single adatom aggregation [8]. In order to identify the key parameters that control the nucleation we used scanning tunneling microscopy (STM) and spot profile analysis low energy electron diffraction (SPA-LEED). It was foundmore » that the number and duration of steps in iterative deposition used to approach θc and the flux rate have dramatic effects on the crystallization process. Larger depositions over shorter times induce greater spatial coverage fluctuations, so local areas can reach the critical coverage θc easier. This can trigger the collective motion of the wetting layer from far away to build the Pb islands “explosively”. Here, the SPA-LEED experiments show that even low flux experiments in iterative deposition experiments can trigger transfer of material to the superstable 7-layer islands, as seen from the stronger satellite rings close to the (00) spot.« less

  15. A combined STM and SPA-LEED study of the “explosive” nucleation and collective diffusion in Pb/Si(111)

    SciTech Connect

    Hattab, H.; Hupalo, M.; Hershberger, M. T.; Horn von Hoegen, M.; Tringides, M. C.

    2015-08-20

    A novel type of very fast nucleation was recently found in Pb/Si(111) with 4- to 7-layer high islands becoming crystalline in an “explosive” way, when the Pb deposited amount in the wetting layer is compressed to θc ~ 1.22 ML, well above the metallic Pb(111) density. This “explosive” nucleation is very different from classical nucleation when island growth is more gradual and islands grow in size by single adatom aggregation [8]. In order to identify the key parameters that control the nucleation we used scanning tunneling microscopy (STM) and spot profile analysis low energy electron diffraction (SPA-LEED). It was found that the number and duration of steps in iterative deposition used to approach θc and the flux rate have dramatic effects on the crystallization process. Larger depositions over shorter times induce greater spatial coverage fluctuations, so local areas can reach the critical coverage θc easier. This can trigger the collective motion of the wetting layer from far away to build the Pb islands “explosively”. Here, the SPA-LEED experiments show that even low flux experiments in iterative deposition experiments can trigger transfer of material to the superstable 7-layer islands, as seen from the stronger satellite rings close to the (00) spot.

  16. A retroelement modifies pre-mRNA splicing: the murine Glrb(spa) allele is a splicing signal polymorphism amplified by long interspersed nuclear element insertion.

    PubMed

    Becker, Kristina; Braune, Marlen; Benderska, Natalya; Buratti, Emanuele; Baralle, Francisco; Villmann, Carmen; Stamm, Stefan; Eulenburg, Volker; Becker, Cord-Michael

    2012-09-01

    The glycine receptor-deficient mutant mouse spastic carries a full-length long interspersed nuclear element (LINE1) retrotransposon in intron 6 of the glycine receptor β subunit gene, Glrb(spa). The mutation arose in the C57BL/6J strain and is associated with skipping of exon 6 or a combination of the exons 5 and 6, thus resulting in a translational frameshift within the coding regions of the GlyR β subunit. The effect of the Glrb(spa) LINE1 insertion on pre-mRNA splicing was studied using a minigene approach. Sequence comparison as well as motif prediction and mutational analysis revealed that in addition to the LINE1 insertion the inactivation of an exonic splicing enhancer (ESE) within exon 6 is required for skipping of exon 6. Reconstitution of the ESE by substitution of a single residue was sufficient to prevent exon skipping. In addition to the ESE, two regions within the 5' and 3' UTR of the LINE1 were shown to be critical determinants for exon skipping, indicating that LINE1 acts as efficient modifier of subtle endogenous splicing phenotypes. Thus, the spastic allele of the murine glycine receptor β subunit gene is a two-hit mutation, where the hypomorphic alteration in an ESE is amplified by the insertion of a LINE1 element in the adjacent intron. Conversely, the LINE1 effect on splicing may be modulated by individual polymorphisms, depending on the insertional environment within the host genome.

  17. Molecular diversity and high virulence of Legionella pneumophila strains isolated from biofilms developed within a warm spring of a thermal spa

    PubMed Central

    2013-01-01

    Background Several cases of legionellosis have been diagnosed in the same French thermal spa in 1986, 1994 and 1997. L. pneumophila serogroup 1 (Lp1) strains have been isolated from several patients, but the source of contamination was not identified despite the presence of different Lp1 in water samples of the three natural springs feeding the spa at this period. Results Our strategy was to investigate L. pneumophila (Lp) strains from natural biofilms developed in a sulphur-rich warm spring of this contaminated site. Biofilm analysis revealed the presence of three Lp serogroups (Lp1, Lp10 and Lp12). Surprisingly, Lp10 and Lp12 were not reported in the previous described studies from water samples. Besides, the new seven Lp1 we isolated exhibit a high molecular diversity and have been differentiated in five classes according to their DNA genome patterns obtained by PFGE and mip sequences. It must be noted that these DNA patterns are original and unknown in databases. Interestingly, the 27 Lp environmental strains we isolated display a higher cytotoxicity and virulence towards the amoeba Acanthamoeba castellanii than those of known Lp1 epidemic strains. Conclusion The characteristics of Legionella pneumophila Lp1 strains isolated from the warm spring are in agreement with their presence in biofilms and their probable long-term persistence in this ecosystem. PMID:23350929

  18. Spa, springs and safety.

    PubMed

    Sukthana, Yaowalark; Lekkla, Amorn; Sutthikornchai, Chantira; Wanapongse, Paitoon; Vejjajiva, Athasit; Bovornkitti, Somchai

    2005-01-01

    Natural mineral water has long been used worldwide for bathing and health purposes. At present, Thailand is famous for health spas and natural hot springs among local people and tourists. Due to possible risks of exposure to harmful agents, we studied hazardous pollutants at 57 natural hot springs from 11 provinces in northern, central, eastern and southern Thailand. Pathogenic, free-living amebae of the genera Naegleria and Acanthamoeba, which can cause central nervous system infection, were found in 26.3% (15/57) and 15.8% (9/ 57), respectively. Dissolved radon, a soil gas with carcinogenic properties, was present in nearly all hot springs sites, with concentration ranging from 0.87-76,527 Becquerels/m3. There were 5 water samples in which radon concentration exceeded the safety limit for drinking. Legionella pneumoniphila (serogroups 1, 3, 5, 6, 7 10 and 13) were found in samples from 71.9% (41/57) of studied sites. Because spas and natural springs are popular tourist attractions, health authorities should be aware of possible hazards and provide tactful measures and guidelines to ensure safety without causing undue alarm to foreign and Thai tourists.

  19. Geohistorical analysis of Paradox Basin

    SciTech Connect

    Lemke, L.D.

    1985-05-01

    The Paradox basin is an elongate sedimentary basin, asymmetric in profile, extending across common corners of Utah, Colorado, Arizona, and New Mexico. Subsidence of the basin began in Desmoinesian time and was coincident with the development of the ancestral Rocky Mountains. The Uncompahgre uplift formed the northeast boundary of the basin during Pennsylvanian and Permian times. Formation thickness and lithologies were obtained from lithologic and radioactivity logs from various parts of the basin. The stratigraphic column at each well, restored through the Upper Cretaceous, was back-stripped and decompacted to reconstruct its depositional history. Decompacted geohistory diagrams and residual (tectonic) subsidence curves were then generated for each well. The Mobil 1 McCormick well, drilled in 1977, penetrates Pennsylvanian strata beneath reverse-faulted granitic basement; this indicates that the basin was flexed down in response to pennsylvanian and Permian thrust faulting along the flank of the Uncompahgre uplift. However, close correspondence of the residual subsidence curves to theoretical thermal subsidence curves indicates that the basin formed by crustal extension. Consequently, development of the basin may have involved crustal stretching (transtensional.) beneath the basin floor, followed by thrusting (transpressional.) along the flank of the Uncompahgre uplift.

  20. Delineating the major KREEP-bearing terranes on the moon with global measurements of absolute thorium abundances

    SciTech Connect

    Lawrence, D.J.; Feldman, W.C.; Barraclough, B.L.; Elphic, R.C.; Prettyman, T.H.; Binder, A.B.; Maurice, S.; Miller, M.C.

    1999-03-01

    The Lunar Prospector (LP) Gamma-Ray Spectrometer (GRS) has been used to map the global composition of thorium on the lunar surface. Previous LP results of relative thorium abundances demonstrated that thorium is highly concentrated in and around the nearside western maria and less so in the South Pole Aitken (SPA) basin. Using new detector modeling results and a larger data set, the authors present here a global map of absolute thorium abundances on a 2{degree} by 2{degree} equal-area pixel scale. Because thorium is a tracer of KREEP-rich material, these data provide fundamental information regarding the locations and importance of terranes that are rich in KREEP bearing materials.

  1. Lunar Anorthosite Deposits: The Big Picture

    NASA Astrophysics Data System (ADS)

    Peterson, C. A.; Hawke, B. R.; Lucey, P. G.; Taylor, J. G.; Blewett, D. T.; Spudis, P. D.

    2000-10-01

    Evidence strongly suggests that Earth's Moon, early in its history, was covered with a deep magma ocean. As the magma cooled and crystallized, pyroxene and olivine grains settled to the bottom, and eventually plagioclase feldspar formed a floatation crust. At first, the continuing flux of impactors easily breached the thin but growing floatation crust and mixed it with magma from below. Crust thick enough to resist further penetration became underplated with pure anorthosite (rock containing at least 90 Analysis of Earth-based near-IR reflection spectra of features on the Moon's nearside has revealed the locations of outcrops of pure anorthosite that may be remnants of the flotation crust. Nearside anorthosite deposits very commonly are found on or very near basin rings. Anorthosites in the inner rings of basins such as Humorum, Grimaldi, and Orientale were derived from beneath more mafic-rich layers in the pre-impact target sites. In addition, the anorthosites associated with the outer rings of Nectaris and other basins are found in the central peaks and walls of large impact craters and were derived from layers deep beneath the crater target sites. In the early 1990s, two spacecraft, Galileo and Clementine, returned multispectral data sets that allow further investigation of the distribution of anorthosite on the Moon, including areas of the farside for which no previous multispectral data existed. Iron maps derived from these data suggest that pure anorthosite may be quite abundant on certain farside surfaces. Farside anorthosite distribution may be largely related to the emplacement of the giant South Pole-Aitken (SPA) basin. Near the basin rim, thick blankets of SPA ejecta appear to cover the original crust, while in more distal regions the thinner cover of SPA ejecta (and mafic-contaminated upper crust) has been completely scattered away by subsequent impacts to reveal the underlying anorthosite.

  2. Great Basin paleontological database

    USGS Publications Warehouse

    Zhang, N.; Blodgett, R.B.; Hofstra, A.H.

    2008-01-01

    The U.S. Geological Survey has constructed a paleontological database for the Great Basin physiographic province that can be served over the World Wide Web for data entry, queries, displays, and retrievals. It is similar to the web-database solution that we constructed for Alaskan paleontological data (www.alaskafossil.org). The first phase of this effort was to compile a paleontological bibliography for Nevada and portions of adjacent states in the Great Basin that has recently been completed. In addition, we are also compiling paleontological reports (Known as E&R reports) of the U.S. Geological Survey, which are another extensive source of l,egacy data for this region. Initial population of the database benefited from a recently published conodont data set and is otherwise focused on Devonian and Mississippian localities because strata of this age host important sedimentary exhalative (sedex) Au, Zn, and barite resources and enormons Carlin-type An deposits. In addition, these strata are the most important petroleum source rocks in the region, and record the transition from extension to contraction associated with the Antler orogeny, the Alamo meteorite impact, and biotic crises associated with global oceanic anoxic events. The finished product will provide an invaluable tool for future geologic mapping, paleontological research, and mineral resource investigations in the Great Basin, making paleontological data acquired over nearly the past 150 yr readily available over the World Wide Web. A description of the structure of the database and the web interface developed for this effort are provided herein. This database is being used ws a model for a National Paleontological Database (which we am currently developing for the U.S. Geological Survey) as well as for other paleontological databases now being developed in other parts of the globe. ?? 2008 Geological Society of America.

  3. Hydrocarbon associations in evaporite basins

    SciTech Connect

    Warren, J.

    1988-01-01

    Evaporite deposition today is not representative of the diversity of scale of evaporites of the past. Ancient evaporites were deposited in two main settings: platform wide or basin wide. Platform evaporites were composed of relatively thin stratiform units (usually <5-10 m thick) deposited on either ramps or behind rimmed shelves. Basinal evaporites were deposited as thick bedded units 10s to 100s of m thick, and laid down in 4 main tectonic settings--rift, collision, transform, and intracratonic. Basins could be further subdivided into three main depositional settings: deep basin-shallow water, deep basin-deep water, and shallow basin-shallow water. Thick basinal salts were remobilized into salt structures in all tectonic settings except intracratonic. Salt flow was due to inherent instability and differential loading in tectonically active settings. Hydrocarbon accumulations associated with these various platforms and basins followed a predictable, but not mutually exclusive, pattern related to the classification of evaporite settings presented in this paper. Reservoirs in platform and ramp settings tended to be of two types--depositional and diagenetic--with most of the diagenesis following patterns predicted by the porosity and plumbing established at or soon after evaporite emplacement. Ramp reservoirs were almost always found in Zone Y, while shelf reservoirs were most common in the grainstone shoals associated with rim or island-crest facies, or their dolomitized equivalents. Reservoirs associated with basinal evaporites were also depositional or diagenetic. Depositional reservoirs were almost all related to topography present during deposition of the carbonates in the basin, often immediately preceding or just beginning evaporitic conditions in the basin.

  4. Atlantic marginal basins of Africa

    SciTech Connect

    Moore, G.T.

    1988-02-01

    The over 10,000-km long Atlantic margin of Africa is divisible into thirty basins or segments of the margin that collectively contain over 18.6 x 10/sup 6/ km/sup 3/ of syn-breakup and post-breakup sediments. Twenty of these basins contain a sufficiently thick volume of sediments to be considered prospects. These basins lie, at least partially, within the 200 m isobath. The distribution of source rocks is broad enough to give potential to each of these basins. The sedimentation patterns, tectonics, and timing of events differ from basin to basin and are related directly to the margin's complex history. Two spreading modes exist: rift and transform. Rifting dates from Late Triassic-Early Jurassic in the northwest to Early Cretaceous south of the Niger Delta. A complex transform fault system separated these two margins. Deep-water communication between the two basins became established in the middle Cretaceous. This Mesozoic-Cenozoic cycle of rifting and seafloor spreading has segmented the margin and where observable, basins tend to be bounded by these segments.

  5. Foreland basins and fold belts

    SciTech Connect

    Macqueen, R.W.; Leckie, D.A. )

    1992-01-01

    The papers in this book describe six foreland basins and fold belts in terms of their regional setting, stratigraphy, tectonics, and structure, and their oil and gas systems. All of the basins show general similarities, but each differs significantly in detail from the others, posing something of a problem in terms of arriving at a 'typical' foreland basin and fold belt. Some are major hydrocarbon producers; others are not. The major characteristics of the six foreland basins and fold belts are summarized in Tables 1 through 5, which provide a convenient means of comparing and contrasting these basins and their hydrocarbon resources. The Western Canada foreland basin and fold belt serves as the type example for several reasons. These include: its setting and clear relationship to a major orogene of Mesozoic-Cenozoic age; the fact that it is uncomplicated by later overprinting, segmentation, or cover rocks unlike the Ouachita, Eastern Venezuela, and U.S. Rocky Mountain foreland basins and fold belts); the fact that there is a large volume of publicly available data on the basin and an active exploration and research community; and the fact that it has reasonable oil and gas reserves in a well-defined stratigraphic framework.

  6. Hydrocarbon associations in evaporite basins

    SciTech Connect

    Warren, J.

    1988-02-01

    Evaporite deposition today is not representative of the diversity or scale of evaporites of the past. Ancient evaporites were deposited in two main settings: platform wide or basin wide. Platform evaporites were composed of relatively thin stratiform units (usually <5-10 m thick) deposited on either ramps or behind rimmed shelves. Basinal evaporites were deposited as thick bedded units 10s to 100s of m thick, and laid down in 4 main tectonic settings - rift, collision, transform, and intracratonic. Basins could be further subdivided into three main depositional settings: deep basin-shallow water, deep basin-deep water, and shallow basin-shallow water. Thick basinal salts were remobilized into salt structures in all tectonic settings except intracratonic. Salt flow was due to inherent instability and differential loading in tectonically active settings. Hydrocarbon accumulations associated with these various platforms and basins followed a predictable, but not mutually exclusive, pattern related to the classification of evaporite settings presented in this paper. Reservoirs in platform and ramp settings tended to be of two types - depositional and diagenetic - with most of the diagenesis following patterns predicted by the porosity and plumbing established at or soon after evaporite emplacement.

  7. Ichnofabric and basin analysis

    SciTech Connect

    Bottjer, D.J. ); Droser, M.L. )

    1991-06-01

    Utilization of ichnofabric indices for measuring recorded extent of bioturbation allows comparative studies of ichnofabric between different facies. In vertical sequences, measurements of ichnofabric indices can be normalized to percent of the total thickness measured for each ichnofabric index. These data can be presented as histograms, or ichnograms, when measurements are from strata deposited in a single genetically-defined sedimentary environment. Ichnograms can be used in conjunction with ichnofacies analysis to present a more complete summary of bioturbation in a sedimentary unit. Using a knowledge of the factors which contribute towards producing ichnofabric in different sedimentary environments, the range of possible ichnograms for any environment can be modeled. In addition to ichnograms, an average ichnofabric index also can be calculated as a useful summary characterization of the extent of bioturbation recorded in a sedimentary unit. Through measurement of ichnofabric indices, construction of ichnograms, and calculation of average ichnofabric index, broad-scale summary data are produced that can allow a more complete understanding of the physical and biological dynamics of sedimentary basins, especially when employed in conjunction with other basin analysis approaches.

  8. New Maturin basin, Venezuela

    SciTech Connect

    Martinez, A.R. )

    1989-09-01

    The Maturin basin of eastern Venezuela is an outstanding example of the historical logic of exploration concepts progressively woven into large-scale commercial development. Exploitation of natural asphalt in the basin started with the end of the 19th century. Geological reconnaissance in a systematic way started in 1911; first oil field, Guanoco, was discovered August 15, 1913; first seismic survey was shot in 1934; first giant oil field, Quiriquire, was discovered June 1, 1928. A well completed January 6, 1936, went through a very heavy and viscous tar interval, non-exploitable at the time, the first in the Orinoco Belt field; the preliminary geological evaluation of the 13,000 km{sup 2} accumulation started in 1967, but was done in earnest during 1979-1983. Only one well had been drilled deeper than 5,200 m in 1985, but then for the contrast, the El Furrial trend with many deep giant light crude oil fields was discovered in 1986.

  9. Advanced Chemistry Basins Model

    SciTech Connect

    Blanco, Mario; Cathles, Lawrence; Manhardt, Paul; Meulbroek, Peter; Tang, Yongchun

    2003-02-13

    The objective of this project is to: (1) Develop a database of additional and better maturity indicators for paleo-heat flow calibration; (2) Develop maturation models capable of predicting the chemical composition of hydrocarbons produced by a specific kerogen as a function of maturity, heating rate, etc.; assemble a compositional kinetic database of representative kerogens; (3) Develop a 4 phase equation of state-flash model that can define the physical properties (viscosity, density, etc.) of the products of kerogen maturation, and phase transitions that occur along secondary migration pathways; (4) Build a conventional basin model and incorporate new maturity indicators and data bases in a user-friendly way; (5) Develop an algorithm which combines the volume change and viscosities of the compositional maturation model to predict the chemistry of the hydrocarbons that will be expelled from the kerogen to the secondary migration pathways; (6) Develop an algorithm that predicts the flow of hydrocarbons along secondary migration pathways, accounts for mixing of miscible hydrocarbon components along the pathway, and calculates the phase fractionation that will occur as the hydrocarbons move upward down the geothermal and fluid pressure gradients in the basin; and (7) Integrate the above components into a functional model implemented on a PC or low cost workstation.

  10. Teaching Grammar and Testing Grammar in the English Primary School: The Impact on Teachers and Their Teaching of the Grammar Element of the Statutory Test in Spelling, Punctuation and Grammar (SPaG)

    ERIC Educational Resources Information Center

    Safford, Kimberly

    2016-01-01

    The research examined the impact on teachers of the grammar element of a new statutory test in Spelling, Punctuation and Grammar (SPaG) in primary schools in England. The research aimed to evaluate the nature and the extent of changes to the teaching of grammar and to wider literacy teaching since the introduction of the test in 2013. The research…

  11. Estancia Basin dynamic water budget.

    SciTech Connect

    Thomas, Richard P.

    2004-09-01

    The Estancia Basin lies about 30 miles to the east of Albuquerque, NM. It is a closed basin in terms of surface water and is somewhat isolated in terms of groundwater. Historically, the primary natural outlet for both surface water and groundwater has been evaporation from the salt lakes in the southeastern portion of the basin. There are no significant watercourses that flow into this basin and groundwater recharge is minimal. During the 20th Century, agriculture grew to become the major user of groundwater in the basin. Significant declines in groundwater levels have accompanied this agricultural use. Domestic and municipal use of the basin groundwater is increasing as Albuquerque population continues to spill eastward into the basin, but this use is projected to be less than 1% of agricultural use well into the 21st Century. This Water Budget model keeps track of the water balance within the basin. The model considers the amount of water entering the basin and leaving the basin. Since there is no significant surface water component within this basin, the balance of water in the groundwater aquifer constitutes the primary component of this balance. Inflow is based on assumptions for recharge made by earlier researchers. Outflow from the basin is the summation of the depletion from all basin water uses. The model user can control future water use within the basin via slider bars that set values for population growth, water system per-capita use, agricultural acreage, and the types of agricultural diversion. The user can also adjust recharge and natural discharge within the limits of uncertainty for those parameters. The model runs for 100 years beginning in 1940 and ending in 2040. During the first 55 years model results can be compared to historical data and estimates of groundwater use. The last 45 years are predictive. The model was calibrated to match to New Mexico Office of State Engineer (NMOSE) estimates of aquifer storage during the historical period by

  12. Thermal history of Michigan basin

    SciTech Connect

    Cercone, K.R.

    1984-02-01

    The elevated organic maturity observed in shallowly buried units from the Michigan basin implies that higher temperatures and thicker overburdens once existed in the basin. Evidence from sediment-accumulation rates, regional dips, and maturity of Pennsylvanian-age coals suggests that up to 1,000 m of sediment were removed by erosion prior to the Late Jurassic, when the basin became stable. Geothermal gradients during Paleozoic basin subsidence probably ranged from 35/sup 0/ to 45/sup 0/ C/ km in contrast to the average present value of 25/sup 0/ C/km. Depths to the top of the oil window ranged from 1,900 to 2,300 m during the Paleozoic. Post-Pennsylvanian erosional uplift and further thermal maturation of the basin have combined to raise the top of the oil window to its present level of 500 m.

  13. Stratigraphic modeling of sedimentary basins

    SciTech Connect

    Aigner, T. ); Lawrence, D.T. )

    1990-11-01

    A two-dimensional stratigraphic forward model has been successfully applied and calibrated in clastic, carbonate, and mixed clastic/carbonate regimes. Primary input parameters are subsidence, sea level, volume of clastics, and carbonate growth potential. Program output includes sequence geometries, facies distribution lithology distribution, chronostratigraphic plots, burial history plots, thermal and maturity histories, and crossplots. The program may be used to predict reservoir distribution, to constrain interpretations of well and seismic data, to rapidly test exploration scenarios in frontier basins, and to evaluate the fundamental controls on observed basin stratigraphy. Applications to data sets from Main Pass (US Gulf Coast), Offshore Sarawak (Malaysia), Rub'al Khali basin (Oman), Paris basin (France), and Baltimore Canyon (US East Coast) demonstrate that the program can be used to simulate stratigraphy on a basin-wide scale as well as on the scale of individual prospects.

  14. Higher percentage of CD3⁺CD154⁺ T lymphocytes predicts efficacy of TNF-α inhibitors in active axial SpA patients.

    PubMed

    Lin, Zhiming; Lin, Qu; Liao, Zetao; Li, Qiuxia; Zhang, Fucheng; Wei, Qiujing; Cao, Shuangyan; Gu, Jieruo

    2014-12-01

    The objective of this study was to evaluate which subtypes of T lymphocytes (CD3(+)CD28(+) and CD3(+)CD154(+)) could predict clinical efficacy after TNF-α inhibitor treatment in active axial SpA patients. Patients who fulfilled Assessment of SpondyloArthritis international Society (ASAS) criteria for axial SpA had a BASDAI of ≥40 mm. All patients received TNF-α inhibitor treatment for 12 weeks. ASAS20 was used to evaluate the effect of the treatment at week 12. We detected the percentage of CD3(+)CD28(+) and CD3(+)CD154(+) T lymphocytes on lymphocyte cells in the peripheral blood in patients and healthy controls. We evaluated whether the percentage of the above subtypes of T lymphocytes could predict clinical efficacy by ROC curve analysis. Fifty-eight healthy controls and 74 active axial SpA patients were included. Mean age was 26.28 ± 9.08 and 26.95 ± 8.13 years for healthy controls and patients, respectively (p = 0.767). The percentage of CD3(+)CD154(+) T lymphocytes was significantly higher in axial SpA patients than in healthy controls (1.62 ± 1.89 % vs 0.79 ± 0.52 %, p < 0.0005). At baseline, the percentage of CD3(+)CD154(+) T lymphocytes was significantly higher in HLA-B27((+)) patients than HLA-B27((-)) ones (HLA-B27(+) vs HLA-B27(-):1.77 ± 1.95 % vs 0.41 ± 0.27 %, p = 0.005). Compared with baseline, the percentage of CD3(+)CD154(+) T lymphocytes significantly decreased to 0.87 ± 0.49 % at week 12 (p < 0.0005). Moreover, we found higher percentage of CD3(+)CD154(+) T lymphocytes could predict clinical efficacy of SpA patients with TNF-α inhibitor treatment (AUC = 0.733, p = 0.014). High percentage of CD3(+)CD154(+) is over-expressed on lymphocytes in peripheral blood of active SpA patients and can be down-regulated by TNF-α inhibitor therapy. High-percentage of CD3(+)CD154(+) T lymphocytes may predict clinical efficacy of TNF-α inhibitor treatment in active axial SpA patients.

  15. The deep Ionian Basin revisited

    NASA Astrophysics Data System (ADS)

    Tugend, Julie; Chamot-Rooke, Nicolas; Arsenikos, Stavros; Frizon de Lamotte, Dominique; Blanpied, Christian

    2016-04-01

    The deep Eastern Mediterranean Basins (Ionian and Herodotus) are characterized by thick sedimentary sequences overlying an extremely thinned basement evidenced from different geophysical methods. Yet, the nature of the crust (continental or oceanic) and the timing of the extreme crustal and lithosphere thinning in the different sub-basins remain highly controversial, casting doubts on the tectonic setting related to the formation of this segment of the North Gondwana paleo-margin. We focus on the Ionian Basin located at the western termination of the Eastern Mediterranean with the aim of identifying, characterizing and mapping the deepest sedimentary sequences. We present tentative age correlations relying on calibrations and observations from the surrounding margins and basins (Malta shelf and Escarpment, Cyrenaica margin, Sirte Basin, Apulian Platform). Two-ship deep refraction seismic data (Expanding Spread Profiles from the PASIPHAE cruise) combined with reprocessed reflection data (from the ARCHIMEDE survey) enabled us to present a homogeneous seismic stratigraphy across the basin and to investigate the velocity structure of its basement. Based on our results, and on a review of geological and geophysical observations, we suggest an Upper Triassic-Early Dogger age for the formation of the deep Ionian Basin. The nature of the underlying basement remains uncertain, both highly-thinned continental and slow-spreading type oceanic crust being compatible with the available constraints. The narrow size and relatively short-lived evolution of the Ionian Basin lead us to suggest that it is more likely the remnant of an immature oceanic basin than of a stable oceanic domain. Eventually, upscaling these results at the scale of the Eastern Mediterranean Basins highlights the complex interaction observed between two propagating oceans: The Central Atlantic and Neo-Tethys.

  16. RESERVES IN WESTERN BASINS PART IV: WIND RIVER BASIN

    SciTech Connect

    Robert Caldwell

    1998-04-01

    Vast quantities of natural gas are entrapped within various tight formations in the Rocky Mountain area. This report seeks to quantify what proportion of that resource can be considered recoverable under today's technological and economic conditions and discusses factors controlling recovery. The ultimate goal of this project is to encourage development of tight gas reserves by industry through reducing the technical and economic risks of locating, drilling and completing commercial tight gas wells. This report is the fourth in a series and focuses on the Wind River Basin located in west central Wyoming. The first three reports presented analyses of the tight gas reserves and resources in the Greater Green River Basin (Scotia, 1993), Piceance Basin (Scotia, 1995) and the Uinta Basin (Scotia, 1995). Since each report is a stand-alone document, duplication of language will exist where common aspects are discussed. This study, and the previous three, describe basin-centered gas deposits (Masters, 1979) which contain vast quantities of natural gas entrapped in low permeability (tight), overpressured sandstones occupying a central basin location. Such deposits are generally continuous and are not conventionally trapped by a structural or stratigraphic seal. Rather, the tight character of the reservoirs prevents rapid migration of the gas, and where rates of gas generation exceed rates of escape, an overpressured basin-centered gas deposit results (Spencer, 1987). Since the temperature is a primary controlling factor for the onset and rate of gas generation, these deposits exist in the deeper, central parts of a basin where temperatures generally exceed 200 F and drill depths exceed 8,000 feet. The abbreviation OPT (overpressured tight) is used when referring to sandstone reservoirs that comprise the basin-centered gas deposit. Because the gas resources trapped in this setting are so large, they represent an important source of future gas supply, prompting studies to

  17. New insights into the origin of the B genome of hexaploid wheat: Evolutionary relationships at the SPA genomic region with the S genome of the diploid relative Aegilops speltoides

    PubMed Central

    Salse, Jérome; Chagué, Véronique; Bolot, Stéphanie; Magdelenat, Ghislaine; Huneau, Cécile; Pont, Caroline; Belcram, Harry; Couloux, Arnaud; Gardais, Soazic; Evrard, Aurélie; Segurens, Béatrice; Charles, Mathieu; Ravel, Catherine; Samain, Sylvie; Charmet, Gilles; Boudet, Nathalie; Chalhoub, Boulos

    2008-01-01

    Background Several studies suggested that the diploid ancestor of the B genome of tetraploid and hexaploid wheat species belongs to the Sitopsis section, having Aegilops speltoides (SS, 2n = 14) as the closest identified relative. However molecular relationships based on genomic sequence comparison, including both coding and non-coding DNA, have never been investigated. In an attempt to clarify these relationships, we compared, in this study, sequences of the Storage Protein Activator (SPA) locus region of the S genome of Ae. speltoides (2n = 14) to that of the A, B and D genomes co-resident in the hexaploid wheat species (Triticum aestivum, AABBDD, 2n = 42). Results Four BAC clones, spanning the SPA locus of respectively the A, B, D and S genomes, were isolated and sequenced. Orthologous genomic regions were identified as delimited by shared non-transposable elements and non-coding sequences surrounding the SPA gene and correspond to 35 268, 22 739, 43 397 and 53 919 bp for the A, B, D and S genomes, respectively. Sequence length discrepancies within and outside the SPA orthologous regions are the result of non-shared transposable elements (TE) insertions, all of which inserted after the progenitors of the four genomes divergence. Conclusion On the basis of conserved sequence length as well as identity of the shared non-TE regions and the SPA coding sequence, Ae speltoides appears to be more evolutionary related to the B genome of T. aestivum than the A and D genomes. However, the differential insertions of TEs, none of which are conserved between the two genomes led to the conclusion that the S genome of Ae. speltoides has diverged very early from the progenitor of the B genome which remains to be identified. PMID:19032732

  18. Geophysical survey of Neovolcanic complexes in the first protection zone of the Sliač Spa and the Baková jama

    NASA Astrophysics Data System (ADS)

    Pašiaková, Mariana; Gajdoš, Vojtech; Bučová, Jana; Dérerová, Jana; Straka, Adam; Hlavˇnová, Petra; Brixová, Bibiana

    2014-03-01

    The main purpose of the survey in the 1st protection zone area of the Sliač Spa and the Baková jama was to clarify the geological-tectonical structure. The vertical electric sounding (VES) technique was selected as the main geophysical survey method. Additionally, the soil radon emanometry was carried out to verify tectonic lines' presence. The outcrop of Pre-Tertiary basement was discovered in the form of small isolated island. No tectonic line was identified based on the evaluation of profile radon concentration. The results of geoelectrical measurements are presented in 8 geological-geophysical crosssections. The results and the tectonic lines' courses interpreted by the VES method are drawn in the map of new indications. The isoline maps and 3D model of Pre-Tertiary basement were constructed.

  19. [The external application of "Plastunskaya" fluoride-containing mineral water in the course of the combined spa and health resort-based treatment of deforming osteoarthrosis].

    PubMed

    Mel'Nichuk, L P; Khodasevich, L S

    2015-01-01

    Mineral waters containing fluorine (fluorinated waters) at a concentration in excess of 1 mg per liter are extensively used in the newly developing spa and health resort areas of the Russian Federation. They have been found in Siberia, Trans-Baikal regions, and the Krasnodar Territory (together with the Greater Sochi). These waters are mainly used for drinking as a component of the balneo- therapeutic treatment. In this context, the study of the therapeutic effects of low-mineralized hydrocarbonate-sodium waters containing fluorine in high concentrations is of paramount importance for the substantiation and facilitation of their external application in the framework of the programs of combined spa and health resort-based treatment of various diseases. We have investigated the possibility of the external application of "Plastunskaya" mineral water characterized above all by the high content of fluorine for the external treatment of osteoarthritis in 187 patients at the age varying from 37 to 63 years in the combination with the sparing regimen of physical activity, therapeutic physical exercises, klimatotherapy of moderate intensity, and a balanced diet. The patients were prescribed to take general "Plastunskaya" mineral water baths with the fluoride concentration of 7.4 mg/l at a temperature of 36 C from 7 to 15 minutes in duration for 2 consecutive days with a break on every third day (the total course consisted of 14-16 baths). It was shown that the combined treatment with the use of fluorine-containing mineral water resulted in the significant improvement of the clinical and biochemical parameters characterizing the health status of the patients suffering from deforming osteoarthrosis. PMID:26841530

  20. Transmission of endemic ST22-MRSA-IV on four acute hospital wards investigated using a combination of spa, dru and pulsed-field gel electrophoresis typing.

    PubMed

    Creamer, E; Shore, A C; Rossney, A S; Dolan, A; Sherlock, O; Fitzgerald-Hughes, D; Sullivan, D J; Kinnevey, P M; O'Lorcain, P; Cunney, R; Coleman, D C; Humphreys, H

    2012-11-01

    The transmission of meticillin-resistant Staphylococcus aureus (MRSA) between individual patients is difficult to track in institutions where MRSA is endemic. We investigated the transmission of MRSA where ST22-MRSA-IV is endemic on four wards using demographic data, patient and environmental screening, and molecular typing of isolates. A total of 939 patients were screened, 636 within 72 h of admission (on admission) and 303 >72 h after admission, and 1,252 environmental samples were obtained. Isolates were typed by spa, dru and pulsed-field gel electrophoresis (PFGE) typing. A composite dendrogram generated from the three sets of typing data was used to divide isolates into 'dendrogram groups' (DGs). Ten percent of patients (92/939) were MRSA-positive; 7 % (44/636) on admission and 16 % (48/303) >72 h after admission (p = 0.0007). MRSA was recovered from 5 % of environmental specimens (65/1,252). Most isolates from patients (97 %, 85/88) and the environment (97 %, 63/65) exhibited the ST22-MRSA-IV genotype. Four DGs (DG1, DG4, DG16 and DG17) accounted for 58 % of ST22-MRSA-IV isolates from patients. Epidemiological evidence suggested cross-transmission among 44/92 patients (48 %) but molecular typing confirmed probable cross-transmission in only 11 instances (13 %, 11/88), with the majority of cross-transmission (64 %; 7/11) occurring on one ward. In the setting of highly clonal endemic MRSA, the combination of local epidemiology, PFGE, spa and dru typing provided valuable insights into MRSA transmission.

  1. Aleutian basin oceanic crust

    USGS Publications Warehouse

    Christeson, Gail L.; Barth, Ginger A.

    2015-01-01

    We present two-dimensional P-wave velocity structure along two wide-angle ocean bottom seismometer profiles from the Aleutian basin in the Bering Sea. The basement here is commonly considered to be trapped oceanic crust, yet there is a change in orientation of magnetic lineations and gravity features within the basin that might reflect later processes. Line 1 extends ∼225 km from southwest to northeast, while Line 2 extends ∼225 km from northwest to southeast and crosses the observed change in magnetic lineation orientation. Velocities of the sediment layer increase from 2.0 km/s at the seafloor to 3.0–3.4 km/s just above basement, crustal velocities increase from 5.1–5.6 km/s at the top of basement to 7.0–7.1 km/s at the base of the crust, and upper mantle velocities are 8.1–8.2 km/s. Average sediment thickness is 3.8–3.9 km for both profiles. Crustal thickness varies from 6.2 to 9.6 km, with average thickness of 7.2 km on Line 1 and 8.8 km on Line 2. There is no clear change in crustal structure associated with a change in orientation of magnetic lineations and gravity features. The velocity structure is consistent with that of normal or thickened oceanic crust. The observed increase in crustal thickness from west to east is interpreted as reflecting an increase in melt supply during crustal formation.

  2. K-Basins design guidelines

    SciTech Connect

    Roe, N.R.; Mills, W.C.

    1995-06-01

    The purpose of the design guidelines is to enable SNF and K Basin personnel to complete fuel and sludge removal, and basin water mitigation by providing engineering guidance for equipment design for the fuel basin, facility modifications (upgrades), remote tools, and new processes. It is not intended to be a purchase order reference for vendors. The document identifies materials, methods, and components that work at K Basins; it also Provides design input and a technical review process to facilitate project interfaces with operations in K Basins. This document is intended to compliment other engineering documentation used at K Basins and throughout the Spent Nuclear Fuel Project. Significant provisions, which are incorporated, include portions of the following: General Design Criteria (DOE 1989), Standard Engineering Practices (WHC-CM-6-1), Engineering Practices Guidelines (WHC 1994b), Hanford Plant Standards (DOE-RL 1989), Safety Analysis Manual (WHC-CM-4-46), and Radiological Design Guide (WHC 1994f). Documents (requirements) essential to the engineering design projects at K Basins are referenced in the guidelines.

  3. KE Basin Sludge Flocculant Testing

    SciTech Connect

    Schmidt, Andrew J.; Hallen, Richard T.; Muzatko, Danielle S.; Gano, Sue

    2004-06-23

    In the revised path forward and schedule for the K Basins Sludge Retrieval and Disposal Project, the sludge in K East (KE) Basin will be moved from the floor and pits and transferred to large, free-standing containers located in the pits (so as to isolate the sludge from the basin). When the sludge is pumped into the containers, it must settle fast enough and clarify sufficiently that the overflow water returned to the basin pool will not cloud the water or significantly increase the radiological dose rate to the operations staff as a result of increased suspended radioactive material. The approach being evaluated to enhance sludge settling and speed the rate of clarification is to add a flocculant to the sludge while it is being transferred to the containers. In February 2004, seven commercial flocculants were tested with a specific K Basin sludge simulant to identify those agents that demonstrated good performance over a broad range of slurry solids concentrations. From this testing, a cationic polymer flocculant, Nalco Optimer 7194 Plus (7194+), was shown to exhibit superior performance. Related prior testing with K Basin sludge and simulant in 1994/1996 had also identified this agent as promising. In March 2004, four series of jar tests were conducted with 7194+ and actual KE Basin sludge (prepared by combining selected archived KE sludge samples). The results from these jar tests show that 7194+ greatly improves settling of the sludge slurries and clarification of the supernatant.

  4. Basin development and petroleum potential of offshore Otway basin, Australia

    SciTech Connect

    Williamson, P.E.; O'Brien, G.W.; Swift, M.G.; Scherl, A.S.; Marlow, M.S.; Exon, N.F.; Falvey, D.A.; Lock, J.; Lockwood, K.

    1987-05-01

    The Bass Strait region in southeastern Australia contains three sedimentary basins, which are, from east to west, the Gippsland, Bass, and Otway basins. The offshore Gippsland basin is Australia's most prolific petroleum-producing province and supplies over 90% of the country's production. In contrast, exploration has been unsuccessful in the offshore portion of the Otway basin; 17 wells have been drilled, and although shows of oil and gas have been common, no commercial discoveries have been made. Many of these wells, drilled in the 1960s and 1970s, were sited using poor-quality seismic data and, as a consequence, were frequently off structure. Seismic data quality has, however, improved significantly in recent years. The present study by the Australian Bureau of Mineral Resources (BMR) involved the collection, in the offshore Otway basin, of 3700 km of high-quality, 48-channel seismic reflection data by the BMR research vessel R/V Rig Seismic. These data have been integrated with existing industry seismic data, well data, limited dredged material, and geohistory analyses in a framework study of basin development and hydrocarbon potential in this under-explored area. The offshore Otway basin extends 500 km along the southern coastline and is typically 50 km wide in water depths of less than 200 m. It contains up to 10 km of predominantly late Mesozoic to early Cenozoic sediments, which are overlain by a thin sequence of middle to late Tertiary shelf carbonates. It has been divided into three main structural elements: the Mussel Platform in the east, the central Voluta Trough, and the Crayfish Platform in the west. The basin was initiated at the end of the Jurassic as part of the Bassian rift. Up to 6 km of Lower Cretaceous sediments were deposited prior to breakup at the end of the Early Cretaceous and the onset of sea-floor spreading between Australia and Antarctica.

  5. Tectonic framework of Turkish sedimentary basins

    SciTech Connect

    Yilmaz, P.O. )

    1988-08-01

    Turkey's exploration potential primarily exists in seven onshore (Southeast Turkey platform, Tauride platform, Pontide platform, East Anatolian platform, Interior, Trace, and Adana) basins and four offshore (Black Sea, Marmara Sea, Aegean Sea, and Mediterranean Sea) regional basins formed during the Mesozoic and Tertiary. The Mesozoic basins are the onshore basins: Southeast Turkey, Tauride, Pontide, East Anatolian, and Interior basins. Due to their common tectonic heritage, the southeast Turkey and Tauride basins have similar source rocks, structural growth, trap size, and structural styles. In the north, another Mesozoic basin, the Pontide platform, has a much more complex history and very little in common with the southerly basins. The Pontide has two distinct parts; the west has Paleozoic continental basement and the east is underlain by island-arc basement of Jurassic age. The plays are in the upper Mesozoic rocks in the west Pontide. The remaining Mesozoic basins of the onshore Interior and East Anatolian basins are poorly known and very complex. Their source, reservoir, and seal are not clearly defined. The basins formed during several orogenic phases in mesozoic and Tertiary. The Cenozoic basins are the onshore Thrace and Adana basins, and all offshore regional basins formed during Miocene extension. Further complicating the onshore basins evolution is the superposition of Cenozoic basins and Mesozoic basins. The Thrace basin in the northwest and Adana basin in the south both originate from Tertiary extension over Tethyan basement and result in a similar source, reservoir, and seal. Local strike-slip movement along the North Anatolian fault modifies the Thrace basin structures, influencing its hydrocarbon potential.

  6. Principles of Sedimentary Basin Analysis

    NASA Astrophysics Data System (ADS)

    Stanton, Robert J., Jr.

    Basin analysis is the ultimate act of synthesis in stratigraphy. Its objective is to weave together the diverse strands of information in order to portray the tectonic evolution of a basin, its filling with sediments in a broad range of depositional environments, the subsequent diagenesis and lithification of these sediments, and the localization therein of mineral and petroleum resources. Thus, although the primary emphasis in basin analysis is stratigraphic and sedimentologic in nature, paleontologic, tectonic, and geophysical data, among others, are also integral components of the effort. It is scientifically challenging and, in practical terms, forms the foundation for the exploration for strata-bound resources. The wide range of topics that must be incorporated into a basin analysis requires that it be a group project of specialists and means that it is a difficult subject to teach or to present in a book.

  7. Provenance and basin evolution, Zhada basin, southwestern Tibet

    NASA Astrophysics Data System (ADS)

    Saylor, J.; Decelles, P.; Gehrels, G.; Kapp, P.

    2007-12-01

    The Zhada basin is a late Miocene - Pliocene intermontane basin situated at high elevations in the Himalayan hinterland. The fluvial and lacustrine sediments of the Zhada formation are undeformed and sit in angular unconformity above the deformed Tethyan Sedimentary Sequence (TSS). The basin sits just south of the Indus suture in a structural position occupied elsewhere in the Himalayan orogen by some of the highest mountains on earth, including Everest. The occurrence of a basin at this location demands explanation. Currently, the Sutlej River flows parallel to the structural grain of the Himalaya, westward through the basin, towards the Leo Pargil (Qusum) range. Near the range front it takes a sharp southward turn, cuts across the structural grain of the Himalaya and out into the Gangetic foreland. Palaeocurrent indicators in the lower part of the Zhada formation show that the basin originated as a northwest flowing axial river. Palaeocurrent indicators are consistently northwest oriented, even to within to within 10 km of the Leo Pargil range front in the north-western end of the basin. This implies that at the onset of sedimentation in Zhada basin the Leo Pargil range was not a barrier as it is today. In the upper part of the Zhada formation, palaeocurrent indicators are generally directed towards the centre of the basin. In the central and southern portions of the basin this indicates a transition from an axial, northwest flowing river to prograding fluvial and alluvial fans. However, in the north-western part of the basin the change between lower and upper Zhada formation involves a complete drainage reversal. This change in palaeocurrent orientation is also reflected in the detrital zircon signal from basin sediments. Low in the Zhada formation the detrital zircon signal is dominated by zircons from the Kailash (Gangdese) batholith (or associated extrusives, see below). However, higher in the sections, a local source, either from the TSS or the core of the

  8. 77 FR 45653 - Yakima River Basin Conservation Advisory Group; Yakima River Basin Water Enhancement Project...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-01

    ... Bureau of Reclamation Yakima River Basin Conservation Advisory Group; Yakima River Basin Water... on the structure, implementation, and oversight of the Yakima River Basin Water Conservation Program... of the Water Conservation Program, including the applicable water conservation guidelines of...

  9. Flexural analysis of two broken foreland basins; Late Cenozoic Bermejo basin and Early Cenozoic Green River basin

    SciTech Connect

    Flemings, P.B.; Jordan, T.E.; Reynolds, S.

    1986-05-01

    Lithospheric flexure that generates basin in a broke foreland setting (e.g., the Laramide foreland of Wyoming) is a three-dimensional system related to shortening along basin-bounding faults. The authors modeled the elastic flexure in three dimensions for two broken foreland basins: the early Cenozoic Green River basin and the analogous late Cenozoic Bermejo basin of Argentina. Each basin is located between a thrust belt and a reverse-fault-bounded basement uplift. Both basins are asymmetric toward the basement uplifts and have a central basement high: the Rock Springs uplift and the Pie de Palo uplift, respectively. The model applies loads generated by crustal thickening to an elastic lithosphere overlying a fluid mantle. Using the loading conditions of the Bermejo basin based on topography, limited drilling, and reflection and earthquake seismology, the model predicts the current Bermejo basin geometry. Similarly, flexure under the loading conditions in the Green River basin, which are constrained by stratigraphy, well logs, and seismic profiling and summed for Late Cretaceous (Lance Formation) through Eocene (Wasatch Formation), successfully models the observed geometry of the pre-Lance surface. Basin depocenters (> 4 km for the Green River basin; > 7 km for the Bermejo basin) and central uplifts are predicted to result from constructive interference of the nonparallel applied loads. Their Bermejo model implies that instantaneous basin geometry is successfully modeled by crustal loading, whereas the Green River basin analysis suggests that basin evolution can be modeled over large time steps (e.g., 20 Ma). This result links instantaneous basin geometry to overall basin evolution and is a first step in predicting stratigraphic development.

  10. Basin modeling of Tadla basin, Morocco, for hydrocarbon potential

    SciTech Connect

    Jabour, H.; Nakayama, K.

    1988-09-01

    The Tadla basin, Morocco, is the easternmost major structural subdivision of the Meseta south of the Central Massif. After the basin remained relatively stable through the Paleozoic, movements during the Variscan orogeny divided the basin into two subbasins, a western subbasin where compressional forces formed large anticlines and an eastern subbasin where intense folding and faulting are associated with uplift and erosion. The sedimentary basin fill includes numerous organic shales, which provide the source beds. Also deposited were numerous shallow-water to continental clastics and to a lesser extent carbonates, providing several possible reservoir rocks for hydrocarbons. The results from computer modeling show that the chief formations of hydrocarbon generation are Carboniferous and Silurian. Most of these formations may have generated hydrocarbons before Variscan movements, which probably destroyed preexisting oil and gas traps. The only source rocks in the generation stage from Mesozoic to the present are the upper Visean shales. The western part of the Tadla basin was the most favorable area for the generation and maturation of hydrocarbons of the upper Visean shales. 22 figures.

  11. Paleothermometry of the Sydney Basin

    SciTech Connect

    Middleton, M.F.; Schmidt, P.W.

    1982-07-10

    Evidence from overprinting of magnetizations of Late Permian and Mesozoic rocks and from the rank of Permian coals and Mesozoic phytoclasts (coal particles) suggests that surface rocks in the Sydney Basin, eastern Australia, have been raised to temperatures of the order of 200 /sup 0/C or higher. As vitrinite reflectance, an index of coal rank or coalification, is postulated to vary predictably with temperature and time, estimates of the paleotemperatures in the Sydney Basin based on observed vitrinite reflectance measurements can be made in conjunction with reasonable assumptions about the tectonic and thermal histories of the basin. These estimates give maximum paleotemperatures of present day surface rocks in the range 60--249 /sup 0/C, depending on factors such as location in the basin, the thickness of the sediment eroded, and the maximum paleogeothermal gradient. Higher coal rank and, consequently, larger eroded thicknesses and paleogeothermal gradients occur along the eastern edge of the basin and may be related to seafloor spreading in the Tasman Sea on the basin's eastern margin. A theory of thermal activation of magnetization entailing the dependence of magnetic viscosity on the size distribution of the magnetic grains is used to obtain an independent estimate of the maximum paleotemperatures in the Sydney Basin. This estimate places the maximum paleotemperature in the range 250--300 /sup 0/C along the coastal region. Both coalification and thermal activation of magnetization models provide strong evidence of elevated paleotemperatures, which in places exceed 200 /sup 0/C, and the loss of sediment thicknesses in excess of 1 km due to erosion.

  12. Floor of Hellas Basin

    NASA Technical Reports Server (NTRS)

    2002-01-01

    [figure removed for brevity, see original site]

    With a diameter of roughly 2000 km and a depth of over 7 km, the Hellas Basin is the largest impact feature on Mars. Because of its great depth, there is significantly more atmosphere to peer through in order to see its floor, reducing the quality of the images taken from orbit. This THEMIS image straddles a scarp between the Hellas floor and an accumulation of material at least a half kilometer thick that covers much of the floor. The southern half of the image contains some of this material. Strange ovoid landforms are present here that give the appearance of flow. It is possible that water ice or even liquid water was present in the deposits and somehow responsible for the observed landscape. The floor of Hellas remains a poorly understood portion of the planet that should benefit from the analysis of new THEMIS data.

    Note: this THEMIS visual image has not been radiometrically nor geometrically calibrated for this preliminary release. An empirical correction has been performed to remove instrumental effects. A linear shift has been applied in the cross-track and down-track direction to approximate spacecraft and planetary motion. Fully calibrated and geometrically projected images will be released through the Planetary Data System in accordance with Project policies at a later time.

    NASA's Jet Propulsion Laboratory manages the 2001 Mars Odyssey mission for NASA's Office of Space Science, Washington, D.C. The Thermal Emission Imaging System (THEMIS) was developed by Arizona State University, Tempe, in collaboration with Raytheon Santa Barbara Remote Sensing. The THEMIS investigation is led by Dr. Philip Christensen at Arizona State University. Lockheed Martin Astronautics, Denver, is the prime contractor for the Odyssey project, and developed and built the orbiter. Mission operations are conducted jointly from Lockheed Martin and from JPL, a division of the California Institute of Technology in

  13. [Quality management in a combined clinic - the quality management system according to DIN EN ISO 9001 of the The German Association of Spa Accommodation Resorts e. V. (VdKB)].

    PubMed

    Eckert, H; Schulze, U

    2004-06-01

    The implementation of a quality system in health care organizations offers huge potentials for improvement and development. But the realization of such systems necessitates high investments, which small companies and family businesses often are unable to raise. The German Association of Spa Accommodation Resorts (VdKB) commissioned two consultants to develop a reasonably priced implementation concept of a DIN EN ISO 9001 quality system specially adjusted to small companies and family businesses in medical spa and rehabilitation services, which was successfully accredited in May 2003. This paper describes fundamental requirements in combine management systems, considers the pros and cons of management combines and demonstrates the practical realization of combine requirements on the example of the VdKB-combine system.

  14. Data showing non-conventional HLA-B27 expression in axial joints and gut tissue from B27 transgenic rats, and in frozen and paraffin-fixed synovial SpA tissue.

    PubMed

    Rysnik, Oliwia; McHugh, Kirsty; van Duivenvoorde, Leonie; van Tok, Melissa; Taurog, Joel; Kollnberger, Simon; Baeten, Dominique; Bowness, Paul

    2016-12-01

    Data is presented showing expression of non-conventional (NC) heavy chain forms of B27 in synovial tissues from SpA patients. Data is presented showing the expression patterns of NC-B27 in joint, gastrointestinal and lymphoid tissues from B27 transgenic (TG(1)) rats with M. tuberculosis-induced SpA. Expression of NC-B27 was determined by immunohistochemistry and flow cytometry using HC10 and HD6 antibodies. These data are the extension of the data presented and discussed in "Non-conventional forms of HLA-B27 are expressed in Spondyloarthritis joints and gut tissue" (O. Rysnik, K. McHugh, L. M. van Duivenvoorde, M. N. van Tok, G. Guggino, J. D. Taurog, S. Kollnberger, F. Ciccia, D. L. Baeten, P. Bowness, 2016) [1]. PMID:27642616

  15. Smythii basin topography and comparisons with Orientale

    NASA Technical Reports Server (NTRS)

    Strain, P. L.; El-Baz, F.

    1979-01-01

    The Smythii basin has the most extensive topographic coverage of any lunar multi-ringed basin. Topographic data are used to study the morphology and morphometry of Smythii and to make comparisons with similar basins. The depth of the basin is shown to be over 8 km, which is comparable to that of Orientale. The inner ring reaches heights of 3.4 km, while the intermediate ring exhibits little relief. Lowest points in the basin are related to mare ridges. Basin volume is estimated to be 21 million cubic kilometers. Evidence suggests that significant differences in substrate characteristics may have existed for the Smythii and Orientale impacts.

  16. Analogue models of pull-apart basins

    NASA Astrophysics Data System (ADS)

    McClay, Ken; Dooley, Tim

    1995-08-01

    Sandbox analogue models of pull-apart basins that developed in sedimentary strata above releasing steps in underlying basement faults are characterized by rhombic basins that are flat-bottomed box grabens with a subhorizontal synkinematic basin infill. Steep to nearly vertical, sigmoidal oblique-slip and segmented oblique-extensional faults are the dominant bounding structures of the pull-apart basins. Cross-basin, short-cut faults link the offset principal displacement zones that are characterized by flower structure development. The structural architectures of the physical models compare directly in form and dimensions to natural examples of strike-slip pull-apart basins.

  17. Coping with vegetation dynamics in low-land wetlands - Integration of RS derived interception into the rainfall-runoff model WetSpa

    NASA Astrophysics Data System (ADS)

    Jarosław, J.; Szporak, S.; Verbeiren, B.; Batelaan, O.

    2012-04-01

    The effective protection of wetlands demands knowledge of hydrological processes, which can be appropriately analysed using distributed models. It is eminent that the calibration and verification of distributed models of catchments with significant wetland coverage have to focus on wetland-specific issues such as the hydrological response of natural vegetation, i.e. parameterisation and dynamics of vegetation. An important and useful parameter describing vegetation canopy structure in terrestrial ecosystems is the Leaf Area Index (LAI), which is closely related to photosynthesis, net primary productivity, evapotranspiration and interception storage capacity. LAI can be estimated with remote sensing data, its suitability to derive the actual state of vegetation is high. This study focuses on improving the interception capacity calculation in the distributed hydrological model WetSpa. The main objective is to integrate seasonal LAI data. Not only field measurements, but also remote sensing derived LAI data is integrated into a WetSpa model for the Upper Biebrza catchment (northeast Poland). Biebrza National Park is characterized by a significant coverage of wetland and large variation in vegetation types. The use of remote sensing derived LAI values considerably improves the assessment of the actual status of vegetation and its seasonal dynamics. Landsat Thematic Mapper images are used to represent the different vegetation stages during the growing season (near LAI minimum and LAI maximum). They are analysed and processed to estimate the interception storage capacity of plant communities typical for Biebrza River valley. LAI of different plant communities has been measured using LAI-2000, and empirical relationships between these measurements and several spectral vegetation indices were established using linear and non-linear regression analysis. The vegetation indices with the highest correlation and the strongest linear relationship regarding LAI are NDVI (R2 = 0

  18. Association and frequency of HLA-A, B and HLA-DR genes in south Tunisian patients with spondyloarthritis (SpA).

    PubMed

    Mahfoudh, Nadia; Siala, Mariam; Rihl, Markus; Kammoun, Arwa; Frikha, Faten; Fourati, Hela; Younes, Mohamed; Gdoura, Radhouane; Gaddour, Lilia; Hakim, Faiza; Bahloul, Zouheir; Baklouti, Sofien; Bargaoui, Naceur; Sellami, Sleheddine; Hammami, Adnene; Makni, Hafedh

    2011-08-01

    The aim of this study is to investigate the association of HLA-A, B and HLA-DR gene expression and to assess an association of additional HLA antigens besides HLA-B27 in south Tunisian patients with spondyloarthritis (SpA). Eighty-five patients diagnosed with ankylosing spondylitis (AS, n=68) and reactive arthrithis (ReA, n=17) were selected and compared with 100 healthy controls (HC). HLA class I antigens were typed serologically using microlymphocytotoxicity technique. HLA-DRB1* alleles were studied by polymerase chain reaction amplification with sequence-specific primers. The significance of differences between patients and controls was tested by chi-square analysis. We found significantly increased frequencies of HLA-A3 (30.6%; pC=0.04; OR=2.95), HLA-B27 (62.35%; pC=4.10(-17), OR=53.55), and HLA-DRB1*15 (17.2%; pC=0.026; RR=2.58) alleles in SpA patients compared to HC. The most frequent and strongest association was observed for HLA-B27 in AS (pC=6.6 ×10(-16), OR=52.23). When AS and ReA patients were analysed separately, HLA-DRB1*15 and HLA-A3 were increased only in AS (pC=0.01, OR=2.99 and pC=0.03, OR=3.14, respectively). In ReA patients, HLA-DRB1*04 (p=0.033, pC=NS, OR=2.89) was found to be the most common allele. By analysing the HLA-B27-negative subgroup, HLA-A3 and HLA-DRB1*15 expression was found to be dependent on the presence of HLA-B27. HLA-B27 expression was higher in male (45/53; 85%) as compared to female (8/53; 15%) patients (p=0.03). Apart from HLA-B27, HLA-A3 and HLA-DRB1*15 are the MHC class I and II alleles found most frequent in Tunisian patients with AS, whereas HLA-DRB1*04 was found most frequent in ReA patients. HLA-B27 is more frequent in male than in female patients. PMID:21360006

  19. Stormwater detention basin sediment removal

    SciTech Connect

    Gross, W.E.

    1995-12-31

    In the past, stormwater runoff from landfills has been treated mainly by focusing on reducing the peak storm discharge rates so as not to hydraulically impact downstream subsheds. However, with the advent of stricter water quality regulations based on the Federal Clean Water Act, and the related NPDES and SPDES programs, landfill owners and operators are now legally responsible for the water quality of the runoff once it leaves the landfill site. At the Fresh Kills Landfill in New York City, the world`s largest covering over 2000 acres, landfilling activities have been underway since 1945. With the main objective at all older landfill sites having focused on maximizing the available landfill footprint in order to obtain the most possible airspace volume, consideration was not given for the future siting of stormwater basin structures. Therefore, when SCS Engineers began developing the first comprehensive stormwater management plan for the site, the primary task was to locate potential sites for all the stormwater basins in order to comply with state regulations for peak stormwater runoff control. The basins were mostly constructed where space allowed, and were sized to be as large as possible given siting and subshed area constraints. Seventeen stormwater basins have now been designed and are being constructed to control the peak stormwater runoff for the 25-year, 24-hour storm as required by New York State. As an additional factor of safety, the basins were also designed for controlled discharge of the 100-year, 24 hour storm.

  20. Inversion of Extensional Sedimentary Basins

    NASA Astrophysics Data System (ADS)

    Buiter, Susanne J. H.; Pfiffner, O. Adrian

    The evolution of extensional sedimentary basins is governed by the surrounding stress field and can, therefore, be expected to be highly sensitive to variations in these stresses. Important changes in basin geometry are to be expected in the case of an even short-lived reversal from extension to compression. We investigate the evolu- tion of fold and thrust structures which form in compression after extension, when basin forming processes have come to a complete stop. To this purpose, we use a two- dimensional, viscoplastic model and start our experiments from a pre-existing exten- sional geometry. We illustrate the sensitivity of the evolving structures to inherited extensional geometry, sedimentary and erosional processes, and material properties. One series of our model experiments involves the upper- to middle crust only in order to achieve a high detail in the basin area. We find that our results agree with examples from nature and analogue studies in, among others, the uplift and rotation of syn-rift sediments, the propagation of shear zones into the post-rift sediments and, in specific cases, the development of back-thrusts or basement short-cut faults. We test the out- come of these models by performing a second series of model simulations in which basins on a continental margin are inverted through their progressive approach of a subduction zone. These latter models are on the scale of the whole upper mantle.

  1. Water Accounting from Ungauged Basins

    NASA Astrophysics Data System (ADS)

    Bastiaanssen, W. G.; Savenije, H.

    2014-12-01

    Water scarcity is increasing globally. This requires a more accurate management of the water resources at river basin scale and understanding of withdrawals and return flows; both naturally and man-induced. Many basins and their tributaries are, however, ungauged or poorly gauged. This hampers sound planning and monitoring processes. While certain countries have developed clear guidelines and policies on data observatories and data sharing, other countries and their basin organization still have to start on developing data democracies. Water accounting quantifies flows, fluxes, stocks and consumptive use pertaining to every land use class in a river basin. The objective is to derive a knowledge base with certain minimum information that facilitates decision making. Water Accounting Plus (WA+) is a new method for water resources assessment reporting (www.wateraccounting.org). While the PUB framework has yielded several deterministic models for flow prediction, WA+ utilizes remote sensing data of rainfall, evaporation (including soil, water, vegetation and interception evaporation), soil moisture, water levels, land use and biomass production. Examples will be demonstrated that show how remote sensing and hydrological models can be smartly integrated for generating all the required input data into WA+. A standard water accounting system for all basins in the world - with a special emphasis on data scarce regions - is under development. First results of using remote sensing measurements and hydrological modeling as an alternative to expensive field data sets, will be presented and discussed.

  2. Exploration potential of offshore northern California basins

    SciTech Connect

    Bachman, S.B.; Crouch, J.K.

    1988-01-01

    A series of exploratory wells was drilled in the northern California offshore basins in the 1960s following leasing of federal tracts off northern California, Oregon, and Washington. The drilling, although encountering numerous oil shows, was considered at the time to indicate low prospectivity in an area that extended as far south as the offshore Santa Maria basin. However, subsequent major discoveries in this decade in the offshore Santa Maria basin, such as the Point Arguello field, indicate that these offshore basins may be highly prospective exploration targets. Many of the key features of Monterey production in central and southern California are also present in the offshore basins of northern California. A new 5-year leasing plan has scheduled leasing in the northern California OCS starting in early 1989. The first basins on the schedule, the Point Arena and Eel River basins, differ in some respects. The Point Arena basin is more typical of a Monterey basin, with the potential for fractured chert reservoirs and organic-rich sections, deep burial of basinal sections to enhance the generation of higher gravity oils, and complex folding and faulting. The Eel River basin is more clastic-rich in its gas-producing, onshore extension. Key questions in the Eel River basin include whether the offshore, more distal stratigraphy will include Monterey-like biogenic sediments, and whether the basin has oil potential in addition to its proven gas potential. The Outer Santa Cruz basin shares a similar stratigraphy, structure, and hydrocarbon potential with the Point Arena basin. The Santa Cruz-Bodega basin, also with a similar stratigraphy, may have less exploration potential because erosion has thinned the Monterey section in parts of the basin.

  3. Testing for Basins of Wada

    PubMed Central

    Daza, Alvar; Wagemakers, Alexandre; Sanjuán, Miguel A. F.; Yorke, James A.

    2015-01-01

    Nonlinear systems often give rise to fractal boundaries in phase space, hindering predictability. When a single boundary separates three or more different basins of attraction, we say that the set of basins has theWada property and initial conditions near that boundary are even more unpredictable. Many physical systems of interest with this topological property appear in the literature. However, so far the only approach to study Wada basins has been restricted to two-dimensional phase spaces. Here we report a simple algorithm whose purpose is to look for the Wada property in a given dynamical system. Another benefit of this procedure is the possibility to classify and study intermediate situations known as partially Wada boundaries. PMID:26553444

  4. Oil in the Malvinas Basin

    SciTech Connect

    Galeazzi, J.S.

    1996-08-01

    The Malvinas Basin is petroliferous. The main source rocks are Late Jurassic and Early Cretaceous outer shelf to basinal shales known as the Pampa Rincon and Lower Inoceramus formations. Main reservoirs are fluvial and shallow-marine sandstones of the coeval Springhill Formation. On the western flank of the basin, 17 wells drilled the Cenozoic and Mesozoic column. Three of these wells discovered hydrocarbons within the Springhill Formation, and one discovered oil in Early Paleogene sandstones. Additionally, some wells recorded shows at different levels within the stratigraphic succession. A detailed overview of the drilled portion of the basin permitted the construction of a sequence stratigraphic framework, and yielded clues on a complex history of deformation. Interpretation of facies and stratal stacking and termination patterns determined that the main reservoir and source rocks were deposited in a ramp-style depositional setting. They represent the lower transgressive phase of a Late Jurassic to Early Cretaceous megasequence deposited during the early sag stage of the basin. Alternative reservoirs to the Springhill sandstones include early Paleogene glauconitic sandstones and carbonates, and Miocene deep-water turbidites. Structural trap styles include normal fault features of Jurassic to Early Cretaceous age, and compressional and inverted positive structures due to Neogene compression. Possible combination and stratigraphic traps include: little tested onlap pinchout of Late Jurassic to Early Cretaceous and Paleogene sandstones and untested erosionally truncated Paleogene sandstones; Early Paleogene carbonate buildups and Miocene deep-water turbidite mounds. The understanding of the geology of the western Malvinas Basin is the key to success of exploration in the huge frontier surrounding areas.

  5. Origin of the earth's ocean basins

    NASA Technical Reports Server (NTRS)

    Frex, H.

    1977-01-01

    The earth's original ocean basins were mare-type basins produced 4 billion years ago by the flux of asteroid-sized objects responsible for the lunar mare basins. Scaling upwards from the observed number of lunar basins for the greater capture cross-section and impact velocity of the Earth indicates that at least 50 percent of an original global crust would have been converted to basin topography. These basins were flooded by basaltic liquids in times short compared to the isostatic adjustment time for the basin. The modern crustal dichotomy (60 percent oceanic, 40 percent continental crust) was established early in the history of the earth, making possible the later onset of plate tectonic processes. These later processes have subsequently reworked, in several cycles, principally the oceanic parts of the earth's crust, changing the configuration of the continents in the process. Ocean basins (and oceans themselves) may be rare occurrences on planets in other star systems.

  6. Prevalence of Met-203 type spaA variant in Erysipelothrix rhusiopathiae isolates and the efficacy of swine erysipelas vaccines in Japan.

    PubMed

    Uchiyama, Mariko; Yamamoto, Kinya; Ochiai, Mariko; Yamamoto, Tsukasa; Hirano, Fumiya; Imamura, Saiki; Nagai, Hidetaka; Ohishi, Kouji; Horiuchi, Noriyuki; Kijima, Mayumi

    2014-03-01

    Since 2009, erysipelas infection among pigs in Japan has been increasing. This study investigated the prevalence, and characteristics of Erysipelothrix rhusiopathiae isolates in Japan from 2008 to 2010 and assessed the efficacy of current commercial erysipelas vaccines. Based on polymorphisms in a 432-bp hypervariable region in the surface protective antigen A (spaA) gene, 34 isolates were classified into three groups: (i) Group 1 with methionine at position 203 (Met-203) and isoleucine at position 257 (Ile-257) (18 isolates of serotype 1a and one untypable isolate). (ii) Group 2 with Ile-257 (12 isolates of serotypes 1a, 1b, 2, 10 and 11), and (iii) Group 3 with alanine at position 195 (Ala-195) and Ile-257 (three isolates of serotype 1a). Isolates with Met-203 were highly pathogenic in mice and pigs, causing death in the pig and LD50 values of 0.45-1.45 CFU per mouse. One live and three inactivated commercial E. rhusiopathiae vaccines were evaluated for efficacy against a Met-203 isolate. Almost all mice and pigs that received vaccine survived, while non-vaccinated controls all died within 5 days of the challenge. This indicates that swine erysipelas vaccines might be still effective in protecting animals against the recently prevalent Met-203 isolates in Japan.

  7. High-resolution melting analysis of the spa locus reveals significant diversity within sequence type 93 methicillin-resistant Staphylococcus aureus from northern Australia.

    PubMed

    Tong, S Y C; Lilliebridge, R A; Holt, D C; McDonald, M I; Currie, B J; Giffard, P M

    2009-12-01

    High-resolution melting analysis is an inherently robust, easy and inexpensive approach to the examination of genomic regions containing single-nucleotide polymorphisms and hypervariable loci. Staphylococcus aureus sequence type (ST) 93 is a singleton, Panton-Valentine leukocidin-positive clone unique to Australia. A high-resolution melting-based method for the identification of ST93 was developed, and a similar approach was used to reveal diversity within the spa locus of this lineage. Statistical and graphical methods that account for instrumental and operator-dependent variation in high-resolution melting curves were developed, to allow greater confidence and reproducibility in deciding whether another curve is truly different from the baseline curve of an amplicon with known sequence. The data support a very early acquisition, or multiple independent acquisitions, of SCCmec by ST93 methicillin-susceptible S. aureus (MSSA), and the coexistence of MSSA and methicillin-resistant S. aureus versions of the same lineage within northern Australia.

  8. H-Area Seepage Basins

    SciTech Connect

    Stejskal, G.

    1990-12-01

    During the third quarter of 1990 the wells which make up the H-Area Seepage Basins (H-HWMF) monitoring network were sampled. Laboratory analyses were performed to measure levels of hazardous constituents, indicator parameters, tritium, nonvolatile beta, and gross alpha. A Gas Chromatograph Mass Spectrometer (GCMS) scan was performed on all wells sampled to determine any hazardous organic constituents present in the groundwater. The primary contaminants observed at wells monitoring the H-Area Seepage Basins are tritium, nitrate, mercury, gross alpha, nonvolatile beta, trichloroethylene (TCE), tetrachloroethylene, lead, cadmium, arsenic, and total radium.

  9. Caribbean basin framework, 4: Maracaibo basin, northwestern Venezuela

    SciTech Connect

    Lugo, J. )

    1991-03-01

    The Maracaibo basin is presently located in a topographic depression on the Maracaibo block, a triangular, fault-bounded block within the Caribbean-South America plate boundary of northwestern Venezuela. Intense oil exploration over the last 50 years has produced a large amount of seismic and well data that can be used to constrain four Jurassic to Recent tectonic and depositional events that affected the region: (1). Late Jurassic rift phase and subsidence along normal faults striking north-northeast across the floor of the basin; (2) Cretaceous to early Eocene subsidence recorded by shallow to deep marine carbonate and clastic rocks that thicken from south to north and completely cover Permian rocks of the Merida arch; (3) Eocene folding, thrusting, and initial reactivation of Jurassic normal faults as convergent strike-slip and reverse faults. Eocene clastic sediments are thickest in a narrow northwest-trending foredeep on the northeastern margin of the basin; (4) Late Miocene to Recent northwest-southeast convergence is marked by continued reactivation of Jurassic normal faults as reverse and left-lateral strike-slip faults, uplift of mountain ranges bordering the basin, and deposition of up to 10 km of clastic sediment.

  10. Supplementary information on K-Basin sludges

    SciTech Connect

    MAKENAS, B.J.

    1999-03-15

    Three previous documents in this series have been published covering the analysis of: K East Basin Floor and Pit Sludge, K East Basin Canister Sludge, and K West Basin Canister Sludge. Since their publication, additional data have been acquired and analyses performed. It is the purpose of this volume to summarize the additional insights gained in the interim time period.

  11. 33 CFR 401.48 - Turning basins.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Turning basins. 401.48 Section... TRANSPORTATION SEAWAY REGULATIONS AND RULES Regulations Seaway Navigation § 401.48 Turning basins. No vessel... the locations set out in the table to this section. Table 1. South Shore Canal: (a) Turning Basin...

  12. 33 CFR 401.48 - Turning basins.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Turning basins. 401.48 Section... TRANSPORTATION SEAWAY REGULATIONS AND RULES Regulations Seaway Navigation § 401.48 Turning basins. No vessel... the locations set out in the table to this section. Table 1. South Shore Canal: (a) Turning Basin...

  13. IMPROVEMENTS IN PUMP INTAKE BASIN DESIGN

    EPA Science Inventory

    Pump intake basins (or wet wells or pump sumps) designed in accordance with accepted criteria often pose many operation and maintenance problems. The report summarizes field surveys of three trench-type pump intake basins representative of 29 such basins that have been in satisfa...

  14. BASIN: Beowulf Analysis Symbolic INterface

    NASA Astrophysics Data System (ADS)

    Vesperini, Enrico; Goldberg, David M.; McMillan, Stephen L. W.; Dura, James; Jones, Douglas

    2013-08-01

    BASIN (Beowulf Analysis Symbolic INterface) is a flexible, integrated suite of tools for multiuser parallel data analysis and visualization that allows researchers to harness the power of Beowulf PC clusters and multi-processor machines without necessarily being experts in parallel programming. It also includes general tools for data distribution and parallel operations on distributed data for developing libraries for specific tasks.

  15. Evolution of the Congo Basin

    NASA Astrophysics Data System (ADS)

    Glasmacher, U. A.; Bauer, F. U.; Kollenz, S.; Delvaux, D.

    2012-04-01

    The Congo Basin is one of the largest basins in the World with very little knowledge on the geological evolution as well as the oil and gas potential. In the past, oil seeps are recorded in the central part of the basin. Four sides in the Congo basin have been drilled so far. The cores of the two drill sides Dekese and Samba are located at the Musée royal de l'Afrique Centrale, Belgium. In a reconnaissance survey, we sampled both drill cores in a nearly even spacing of ~ 150 m covering the whole stratigraphy from Albian to Proterozoic. The red and green to grey sandstone samples were prepared by usual heavy minerals separation technique. Most of the samples revealed enough apatite and zircon grains for the two thermochronometric techniques fission track and (U-Th-Sm)/He. The time-temperature (t-T) evolution for the two drill locations were modelled by using the determined thermochronological data within the software code HeFTy. We tested various geological evolutionary constrains. Both techniques provide us information on the thermal and exhumation of the possible source area and on the drill location by themselves.

  16. AR-40 AR-39 Age of an Impact-Melt Lithology in DHOFAR 961

    NASA Technical Reports Server (NTRS)

    Frasl, B.; Cohen, B. A.; Li, Z.-H.; Jolliff, B.; Korotev, R.; Zeigler, R.

    2016-01-01

    The South Pole-Aitken (SPA) basin is the stratigraphically oldest identifiable lunar basin and is therefore one of the most important targets for absolute age-dating to help understand whether ancient lunar bombardment history smoothly declined or was punctuated by a cataclysm. The SPA basin also has another convenient property, a geochemically distinct interior, unobscured by extensive mare basalt fill. A case has been made for the possible origin of the Dhofar 961 lunar meteorite in the South Pole-Aitken (SPA) basin, based on comparing its composition with Lunar Prospector gamma-ray data for the interior of the SPA basin. Dhofar 961 contains several different impact-melt (IM) lithologies. Jolliff et al. described two classes of mafic impact-melt lithologies, one dominated by olivine (Lithology A) and the other by plagioclase (An 95-96.5) (Lithology B). Broad-beam analyses of these lithologies yielded (is) approximately 14.0 wt% FeO, 11.7 wt% MgO, and 15.4 wt% Al2O3. Lithologies A and B differ by approximately 2.5% Al2O3, 1.5% FeO and 1.5% MgO, consistent with the occurrence of olivine phenocrysts in A and plagioclase clasts in B. Both lithologies are considerably more mafic than the Apollo mafic impact-melt breccias, corresponding to olivine gabbronorite. Joy et al. used U-Pb dating to investigate phosphate fragments in the Dhofar 961 matrix and impact-melt clasts. Matrix phosphates have 4.34 to 4 Ga ages, consistent with ancient KREEP-driven magmatic episodes and Pre-Nectarian ((is) greater than 3.92 Ga). Phosphates found within Dhofar 961 crystalline impact melt breccia clasts range from 4.26 to 3.89 Ga, potentially recording events throughout the basin forming epoch of lunar history. The youngest reset ages in the Dhofar 961 sample represent an upper limit for the time of formation of the meteorite. Joy et al suggested this age represents the final impact that mixed and consolidated several generations of precursor rocks into the Dhofar meteorite group

  17. Tectonic development of Michigan basin

    SciTech Connect

    Prouty, C.E.

    1986-08-01

    The general form of the Michigan basin and surrounding frame structures - the Findlay, Kankakee, and Wisconsin arches - was inherited from the Precambrian. An ongoing study has provided new information on present basin configuration and the evolution of intrabasinal structures during the Paleozoic. This study involves: (1) isopach, structure contour, depocenter, and lithofacies map preparation; (2) diagenetic and epigenetic dolomitization processes and patterns; (3) Landsat imagery and lineament interpretation; (4) recognition of shearing mechanics and the resulting shear faulting and folding; and (5) the recognition of radial faults in contrast to shear faults. Monitoring of the above throughout the Paleozoic indicates that tectonic events within the basin were episodic in nature. Stresses are recognized as external and, through Fourier analysis of lineaments (shear faults), may be demonstrated as from the southeast, probably the Appalachian mobile belt. Shear faults are seated in Precambrian rocks, although they are probably not of that age. The faults occur with accompanying shear folds in rocks possibly as early as the Late Ordovician or Middle Silurian, but definitely by the Middle Devonian with the principal faulting and folding during the post-Osage Mississippian. Local shifting of the depocenter within the general Saginaw Bay area occurred during the early Paleozoic with a major shift westward to the present central basin position accompanied by the development of the present north-northwest ellipticity of the basin during the post-Osage, pre-Meramecian Mississippian. Barrier separation of the West Michigan Lagoon occurred in the Middle Ordovician and Middle and Late Devonian. Radial structures can be demonstrated in at least the Upper Silurian and Upper Devonian.

  18. Automated basin delineation from digital terrain data

    NASA Technical Reports Server (NTRS)

    Marks, D.; Dozier, J.; Frew, J.

    1983-01-01

    While digital terrain grids are now in wide use, accurate delineation of drainage basins from these data is difficult to efficiently automate. A recursive order N solution to this problem is presented. The algorithm is fast because no point in the basin is checked more than once, and no points outside the basin are considered. Two applications for terrain analysis and one for remote sensing are given to illustrate the method, on a basin with high relief in the Sierra Nevada. This technique for automated basin delineation will enhance the utility of digital terrain analysis for hydrologic modeling and remote sensing.

  19. Tectonic evolution and oil and gas of Tarim basin

    NASA Astrophysics Data System (ADS)

    Yuzhu, Kang; Zhihong, Kang

    According to the new results achieved in the past ten years and more, using mobilism and the theory of polycycle by Huang Jiqing (1977, 1984), the formation of the basement of the Tarim basin and its characteristics are summarized. The prototype basins formed since Sinian times are classified into rift basin, continental marginal basin, cratonic basin, foreland basin and others. The Tarim basin is regarded as a huge oil- and gas-bearing basin superposed by prototype basins of different ages. The tectonic characteristics of these basins including tectonic movements, tectonic migrations, faults and trap types are summarized. In addition, structural control over oil and gas and oil-forming features are analysed.

  20. Mississippian facies relationships, eastern Anadarko basin, Oklahoma

    SciTech Connect

    Peace, H.W. ); Forgotson, J.M. )

    1991-08-01

    Mississippian strata in the eastern Anadarko basin record a gradual deepening of the basin. Late and post-Mississippian tectonism (Wichita and Arbuckle orogenies) fragmented the single large basin into the series of paired basins and uplifts recognized in the southern half of Oklahoma today. Lower Mississippian isopach and facies trends (Sycamore and Caney Formations) indicate that basinal strike in the study area (southeastern Anadarko basin) was predominantly east-west. Depositional environment interpretations made for Lower Mississippian strata suggest that the basin was partially sediment starved and exhibited a low shelf-to-basin gradient. Upper Mississippian isopach and facies trends suggest that basinal strike within the study area shifted from dominantly east-west to dominantly northwest-southeast due to Late Mississippian and Early Pennsylvanian uplift along the Nemaha ridge. Within the study area, the Chester Formation, composed of gray to dove-gray shales with interbedded limestones deposited on a carbonate shelf, thins depositionally into the basin and is thinnest at its facies boundary with the Springer Group and the upper portion of the Caney Formation. As basin subsidence rates accelerated, the southern edge of the Chester carbonate shelf was progressively drowned, causing a backstepping of the Chester Formation calcareous shale and carbonate facies. Springer Group sands and black shales transgressed northward over the drowned Chester Formation shelf.

  1. Geologic Mapping of the Lunar South Pole Quadrangle (LQ-30)

    NASA Technical Reports Server (NTRS)

    Mest, S. C.; Berman, D. C.; Petro, N. E.

    2010-01-01

    In this study we use recent image, spectral and topographic data to map the geology of the lunar South Pole quadrangle (LQ-30) at 1:2.5M scale [1-7]. The overall objective of this research is to constrain the geologic evolution of LQ-30 (60 -90 S, 0 - 180 ) with specific emphasis on evaluation of a) the regional effects of impact basin formation, and b) the spatial distribution of ejecta, in particular resulting from formation of the South Pole-Aitken (SPA) basin and other large basins. Key scientific objectives include: 1) Determining the geologic history of LQ-30 and examining the spatial and temporal variability of geologic processes within the map area. 2) Constraining the distribution of impact-generated materials, and determining the timing and effects of major basin-forming impacts on crustal structure and stratigraphy in the map area. And 3) assessing the distribution of potential resources (e.g., H, Fe, Th) and their relationships with surface materials.

  2. Geodynamics of the Sivas Basin (Turkey): from a forearc basin to a retroarc foreland basin

    NASA Astrophysics Data System (ADS)

    Legeay, Etienne; Ringenbach, Jean-Claude; Kergaravat, Charlie; Callot, Jean-Paul; Mohn, Geoffroy; Kavak, Kaan

    2016-04-01

    Anatolia records the consumption of several oceanic basins, from the Northern Neotethys domain, by north-dipping subduction until the end of Mesozoic. The associated obduction event occurred during Campanian, from North to South and from Greece to Oman, leading to the emplacement of ophiolite thrust sheets and associated ophiolitic mélange. In particular, the Sivas Basin in Eastern Anatolia is located at the boundary between the Kırsehir block to the East, Pontide arc to the North and Tauride Platform to the South, sutured by ophiolitic belts. The Sivas Basin formed a Tertiary fold-and-thrust belt, which exhibits mainly north verging thrust in Paleogene deposits, and South verging thrust in oligo-miocene sequence. To understand the northern verging thrust above south verging obduction, it is necessary to zoom out of the basin, and include a set of processes that affect the eastern Anatolia. This study aims to characterize the structural and sedimentary evolution of the Sivas Basin, based on a fieldwork approach, coupled to the interpretation of subsurface data, thermochronology and biostratigraphy. The Sivas Basin was initiated in a forearc setting relatively to the subduction of the Inner-Tauride Ocean while the associated ophiolites are obducted onto the northern passive margin of the Tauride margin. Early Maastrichtian to Paleocene deposits are represented by carbonate platforms located on ophiolitic highs, passing to turbidites and olistostomes toward the North. The early Eocene sediments, mainly composed of ophiolitic clasts, are deposited on a regional unconformity marked along the southern margin of the basin by incisions in response to the emergence of north-verging thrust. The middle Eocene sediments, intensively folded by northward thrusting, are mostly represented by flysch type deposits (olistostromes, mass-flows and turbidites). The onset of the compression is related to the initiation of the Taurus shortening in a retroarc situation, in response to

  3. Hydrocarbon accumulations in the Tarim basin, China

    SciTech Connect

    Li Desheng; Liang Digang; Jia Chengzao; Wang Gang

    1996-10-01

    The Tarim basin is the largest and least explored inland basin in China. The areal extent of the basin reaches 560,000 km{sup 2}. The interior of the basin is mostly covered by the Takla Mekan Desert, which is about 330,000 km{sup 2} in areal extent. The basin has become the object of special attention since China set aside first- and third-round onshore bidding blocks in the Tarim basin for foreign oil firms to explore. The Tarim basin is a polyhistory superimposed basin that has experienced seven evolutionary stages: (1) Sinian-Cambrian-Ordovician aulacogen stage, (2) Silurian-Devonian intracratonic depression stage, (3) Carboniferous marginal sea stage, (4) Permian rift basin stage, (5) Triassic-Jurassic foreland basin stage, (6) Cretaceous-Paleogene NeoTethys bay stage, and (7) Neogene-Pleistocene foreland and inland basin stage. Both the basin`s Paleozoic marine platform sequences and the Mesozoic-Cenozoic terrestrial fills are believed to contain substantial volumes of hydrocarbons. After recent years of exploration, nine oil and gas fields have been proven and 23 discoveries have been made in the Tabei, Tazhong, and Southwest areas. Kekeya, Lunnan, Sangtamu, Jiefangqudong, Donghetang, and Tazhong 4 oil fields have been put into production. Output of crude oil was 2.6 million t (metric tons) (52,000 BOPD) in 1995. The production will increase to 5 million t (100,000 BOPD) in 1997. Giant oil and gas traps probably will be discovered in the Tarim basin. The prospect is promising.

  4. Keuper stratigraphic cycles in the Paris basin and comparison with cycles in other peritethyan basins (German basin and Bresse-Jura basin)

    NASA Astrophysics Data System (ADS)

    Bourquin, Sylvie; Guillocheau, François

    1996-09-01

    High-resolution sequence stratigraphy of the Keuper, Paris Basin, is used to establish correlations between the basin-centre evaporite series and the basin-margin clastics series. The high-resolution correlations show stratigraphic cycle geometries. The Keuper consists of five minor base-level cycles whth occur in the upper portion of the Scythian-Carnian major base-level cycle and the lower part of the Carnian-Liassic major base-level cycle. The maximum relative rate of subsidence for the base-level fall phase of the Scythian-Carnian major cycle occurs in the eastern part of the Paris Basin. During the base-level rise phase of the Carnian-Liassic major cycle, the area of highest rate of subsidence shifted westwards and northwards. This shift records the first occurrence of an independent Paris Basin which was no longer merely the western margin of the German Basin. Two phases of tectonic movement influenced evaporite sedimentation and sequence geometries by creating areas of subsidence where halite could accumulate. The second, within the 'Marnes irisées supérieures', induced a general westward and northward tilt of the basin. Concurrent migration of depocentres to the west and north produced an intra-'Marnes irisées supérieures' truncation. Comparison of the stratigraphic records of the Paris Basin and of other Triassic Peritethyan basins (German Basin, Bresse-Jura Basin and South-East Basin) reveals numerous similarities. The coastal onlap curve of the German Keuper (Aigner and Bachmann, 1992) exhibits many similarities with the sequence evolution of the Paris Basin. But the Triassic succession is more complete in the German Basin and more cycles are observed. The major difference between these two basins during the Keuper is that the 'Marnes irisées inférieures' minor base-level cycle does not occur in the German Basin. In the Bresse-Jura Basin, the major difference concerns the Lettenkohle. One minor base-level cycle is recorded in the Paris Basin while

  5. Formation of lunar basin rings

    USGS Publications Warehouse

    Hodges, C.A.; Wilhelms, D.E.

    1978-01-01

    The origin of the multiple concentric rings that characterize lunar impact basins, and the probable depth and diameter of the transient crater have been widely debated. As an alternative to prevailing "megaterrace" hypotheses, we propose that the outer scarps or mountain rings that delineate the topographic rims of basins-the Cordilleran at Orientale, the Apennine at Imbrium, and the Altai at Nectaris-define the transient cavities, enlarged relatively little by slumping, and thus are analogous to the rim crests of craters like Copernicus; inner rings are uplifted rims of craters nested within the transient cavity. The magnitude of slumping that occurs on all scarps is insufficient to produce major inner rings from the outer. These conclusions are based largely on the observed gradational sequence in lunar central uplifts:. from simple peaks through somewhat annular clusters of peaks, peak and ring combinations and double ring basins, culminating in multiring structures that may also include peaks. In contrast, belts of slump terraces are not gradational with inner rings. Terrestrial analogs suggest two possible mechanisms for producing rings. In some cases, peaks may expand into rings as material is ejected from their cores, as apparently occurred at Gosses Bluff, Australia. A second process, differential excavation of lithologically diverse layers, has produced nested experimental craters and is, we suspect, instrumental in the formation of terrestrial ringed impact craters. Peak expansion could produce double-ring structures in homogeneous materials, but differential excavation is probably required to produce multiring and peak-in-ring configurations in large lunar impact structures. Our interpretation of the representative lunar multiring basin Orientale is consistent with formation of three rings in three layers detected seismically in part of the Moon-the Cordillera (basin-bounding) ring in the upper crust, the composite Montes Rook ring in the underlying

  6. THE ADVANCED CHEMISTRY BASINS PROJECT

    SciTech Connect

    William Goddard; Peter Meulbroek; Yongchun Tang; Lawrence Cathles III

    2004-04-05

    In the next decades, oil exploration by majors and independents will increasingly be in remote, inaccessible areas, or in areas where there has been extensive shallow exploration but deeper exploration potential may remain; areas where the collection of data is expensive, difficult, or even impossible, and where the most efficient use of existing data can drive the economics of the target. The ability to read hydrocarbon chemistry in terms of subsurface migration processes by relating it to the evolution of the basin and fluid migration is perhaps the single technological capability that could most improve our ability to explore effectively because it would allow us to use a vast store of existing or easily collected chemical data to determine the major migration pathways in a basin and to determine if there is deep exploration potential. To this end a the DOE funded a joint effort between California Institute of Technology, Cornell University, and GeoGroup Inc. to assemble a representative set of maturity and maturation kinetic models and develop an advanced basin model able to predict the chemistry of hydrocarbons in a basin from this input data. The four year project is now completed and has produced set of public domain maturity indicator and maturation kinetic data set, an oil chemistry and flash calculation tool operable under Excel, and a user friendly, graphically intuitive basin model that uses this data and flash tool, operates on a PC, and simulates hydrocarbon generation and migration and the chemical changes that can occur during migration (such as phase separation and gas washing). The DOE Advanced Chemistry Basin Model includes a number of new methods that represent advances over current technology. The model is built around the concept of handling arbitrarily detailed chemical composition of fluids in a robust finite-element 2-D grid. There are three themes on which the model focuses: chemical kinetic and equilibrium reaction parameters, chemical

  7. Near Surface Magnetic Field Mapping over the Swirls in the SPA Region on the Moon Using Kaguya LMAG Low Altitude Data

    NASA Astrophysics Data System (ADS)

    Shibuya, H.; Tsunakawa, H.; Takahashi, F.; Shimizu, H.; Matsushima, M.

    2010-12-01

    We have reported the correlation between the high albedo marking (HAM) on the moon surface and the strength of horizontal component (Bh) of the near surface lunar magnetic field, at 2009 AGU Fall meeting, using the Lunar Prospector magnetometer data (LP-MAG). The correlation is further examined using the lowest altitude data at the latest orbits of Kaguya magnetometer data (KG-MAG). The Kaguya spacecraft (launched on September 14, 2007) dropped to the Moon at 65.5S and 80.4E on Jun 11, 2009. On the last few weeks, it flies over SPA with low altitudes as 10km, and the magnetometer acquired beautiful data. The magnetic field on the Mare Ingenii, on which we can see one of the most enhanced HAM, is restored using equivalent pole reduction (EPR) technique. First, EPR model is examined by comparing the model field and the other observations of LP-MAG and KG-MAG. They agree each other not only the shape but also the amplitude of the peaks. It indicates that the lunar magnetic field is well reproduced in 3-dimension. The magnetic field at the altitude of 5km is mapped over the Mare Ingenii and adjacent region where the HAM is clearly seen from Clementine albedo images. The coincidence of the HAM and the Bh is incredibly well, especially for the HAM in the flat crater floors. In some region, some of the detailed shapes of HAM match with the small bulge in the Bh contour. The HAM seems to be correlated maximas of Bh rather than its absolute strength. This result further support that the HMA is formed by magnetic shielding of the solar wind particles.

  8. Effectiveness of Comprehensive Health Education Combining Lifestyle Education and Hot Spa Bathing for Male White-Collar Employees: A Randomized Controlled Trial with 1-Year Follow-Up

    PubMed Central

    Kamioka, Hiroharu; Nakamura, Yosikazu; Okada, Shinpei; Kitayuguchi, Jun; Kamada, Masamitsu; Honda, Takuya; Matsui, Yuzuru; Mutoh, Yoshiteru

    2009-01-01

    Background Physical activity is known to prevent obesity and metabolic syndrome in middle-aged and elderly people; however, the effectiveness of a comprehensive health education program for male white-collar employees is uncertain. Methods Forty-three men volunteered to participate in this study and were randomly assigned into 2 groups. The intervention group participated in a 2-hour program comprising comprehensive health education and hot spa bathing, offered once every 2 weeks, in addition to individualized programs once a week, for 24 weeks. The control group received only general health guidance. We compared their lifestyle characteristics and physical and mental health criteria at baseline, immediately after the intervention, and 1 year after the end of the intervention. Results Rates of adherence to individualized programs were 60.0 ± 27.2% and 30.5 ± 29.6% at the end of the intervention and at 1 year after the end of the intervention, respectively. Significant (P < 0.05) interaction of criteria was observed for cluster of differentiation 4+ (CD4+) cells and the ratio of cluster of differentiation 4+ to 8+ (CD4/8) cells, which were used to represent the participants' immunological function. We divided the intervention group into 2 subgroups on the basis of their attendance. Among the resulting 3 groups, significant interaction of criteria was observed for CD4+ and CD4/8 cells. In addition, the high attendance group had the highest CD4+ count and CD4/8 ratio. Conclusions Participants who attended classes and/or performed the supplementary individualized programs tended to maintain their immunological function and to experience a decrease in body fat percentage. However, few effects were noted in participants with poor adherence, even in the intervention group. PMID:19687610

  9. Evaluation of the Pseudalert/Quanti-Tray MPN Test for the Rapid Enumeration of Pseudomonas aeruginosa in Swimming Pool and Spa Pool Waters.

    PubMed

    Sartory, David P; Brewer, Megan; Beswick, Agnieszka; Steggles, Darron

    2015-12-01

    This study assessed the performance of a new most probable number test (Pseudalert/Quanti-Tray) for the enumeration of Pseudomonas aeruginosa from swimming pool and spa pool waters by comparing it to the international and national membrane filtration-based culture methods for P. aeruginosa: ISO 16266:2006 and UK The Microbiology of Drinking Water-Part 8 (MoDW Part 8) which both use Pseudomonas CN agar. The comparison was based on the calculation of mean relative differences between the two methods conducted according to ISO 17994:2014. Using both routine pool water samples (149 from 8 laboratories) and artificially contaminated samples (309 from 7 laboratories), paired counts from each sample and enumeration method were analysed. For routine samples, there were insufficient data for a conclusive assessment, but the data do indicate at least equivalent performance of Pseudalert/Quanti-Tray to the reference methods. For the artificially contaminated samples, the data also did not result in a statistically conclusive assessment but did indicate potentially better performance of Pseudalert/Quanti-Tray. Combining the data from the routine samples and artificially contaminated samples resulted in an ISO 17994 outcome that the two methods were not statistically significantly different. Thus, the Pseudalert/Quanti-Tray method is an acceptable alternative to ISO 16266 and MoDW Part 8. The Pseudalert/Quanti-Tray method has the advantage in that it does not require confirmation testing, and of providing confirmed counts within 24-28 h incubation compared to 40-48 h or longer for the ISO 16266 and MoDW Part 8 methods.

  10. Evaluation of Infiltration Basin Performance in Florida

    NASA Astrophysics Data System (ADS)

    Bean, E.

    2012-12-01

    Infiltration basins are commonly utilized to reduce or eliminate urban runoff in Florida. For permitting purposes, basins are required to recover their design volume, runoff from a one inch rainfall event, within 72 hours to satisfy the design criteria and are not required to account for groundwater mounding if volume recovery can be accomplished by filling of soil porosity by vertical infiltration below the basin surface. Forty infiltration basins were included in a field study to determine whether basin hydraulic performance was significantly different from their designed performance. Basins ranged in age from less than one year to over twenty years and land uses were equally divided between Florida Department of Transportation (FDOT) and residential developments. Six test sites within each basin were typically selected to measure infiltration rates using a double ring infiltrometer (DRI), a common method for infiltration basin sizing. Measured rates were statistically compared to designed infiltration rates, taking into account factors of safety. In addition, a surface soil boring was collected from each of the test sites for a series of analyses, including soil texture, bulk density, and organic matter content. Eleven of the 40 evaluated basins were monitored between March 2008 and January 2012 to evaluate whether basins recovered their volumes from one inch events within 72 hours and to evaluate the effectiveness of using DRI rates to evaluate basin performance. Based on DRI rates, 16 (40%) basins had rates less than their designed rates, 10 (25%) had rates equal to their designed rates, and 14 (35%) basins had rates greater than their designed rates. Additionally, basins with coarser soils were also more likely to have DRI rates greater than designs and FDOT basins were more likely than residential basins to have infiltration rates at or above their designed rates. Five of the eleven monitored basins were expected to function as designed by recovering their

  11. The Central European Permian Basins; Rheological and structural controls on basin history and on inter-basin connectivity

    NASA Astrophysics Data System (ADS)

    Smit, Jeroen; Van Wees, Jan-Diederik; Cloetingh, Sierd

    2014-05-01

    We analyse the relative importance of the major crustal-scale fault zones and crustal architecture in controlling basin formation, deformation and the structural connections between basins. The North and South Permian Basins of Central Europe are usually defined by the extend of Rotliegend sedimentary and volcanic units and not by a common tectonic origin or development. Instead, the sub-basins that together form the Permian Basins are each controlled by different structural and/or rheological controls that are inherited from Early Paleozoïc and older geodynamic processes, they are even located in different crustal/lithospheric domains. The North Permian basin is located on Baltic crust that was thinned during Late Proterozoïc - Early Paleozoïc times. South of the Thor suture, the South Permian basin and its sub-basins are located on Avalonian crust (Southern North Sea and North German Basins) and on the transition of East European cratonic and Avalonian crust (Polish Through). The size of crustal domains and of the faults that govern basin formation requires a regional-scale to assess their impact on basins and sub-basins. In the case of the Permian Basins this encompasses East Avalonia and surroundings, roughly speaking the area north of the Variscan Rheïc suture, east of the Atlantic and southwest of the Teisseyre-Tornquist line. This approach sheds light on the effects of long lived differences in crustal fabric which are responsible for spatial heterogeneity in stress and strain magnitudes and zonations of fracturing, burial history and temperature history. The focus on understanding the geomechanical control of large crustal-scale fault structures will provide the constraints and geometrical and compositional input for local models of stress and strain. Considering their fundamentally different structural and rheological controls, the Permian (sub)basins have a remarkably common history of subsidence and inversion, suggesting a more or less continuous

  12. CLEAR LAKE BASIN 2000 PROJECT

    SciTech Connect

    LAKE COUNTY SANITATION DISTRICT

    2003-03-31

    The following is a final report for the Clear Lake Basin 2000 project. All of the major project construction work was complete and this phase generally included final details and testing. Most of the work was electrical. Erosion control activities were underway to prepare for the rainy season. System testing including pump stations, electrical and computer control systems was conducted. Most of the project focus from November onward was completing punch list items.

  13. Environmental change in the Mississippi River Basin

    NASA Astrophysics Data System (ADS)

    Xu, X.; Wynn, G.; Hassan, M. A.; Donner, S. D.; Sivapalan, M.

    2010-12-01

    Land use, land cover change and hydrological modification are important factors affecting discharge, sediment yield, nutrient flows and precipitation at small and large scales. This presentation analyses the changes in crop and pasture land as well as dam and reservoir construction from 1900 to the present in the Mississippi River Basin (including six main sub-basins), to assess their influence on sediment and nutrient dynamics in the basin. Total cropland and pastureland from 1900-2007 are characterized at 0.5 degree x 0.5 degree spatial resolution from existing satellite-derived datasets. From 1900s to 2000s, total cropland in the Ohio River Basin and the Tennessee River Basin in the east exhibited a decreasing trend. The other sub-basins and the basin as a whole exhibited an increasing trend. The area under pasture in the Ohio, the Tennessee and the Upper Mississippi river basins decreased; it increased in the other sub-basins. The areas of corn, wheat and soybean, the three dominant crops in the United States, from 1950 to 2000 are characterized at 5’ x 5’ spatial resolution from existing inventory and satellite-data. The fractional coverage of soybean and wheat increased in most sub-basins, whereas the fraction of corn remained constant or decreased in most sub-basins. The distribution of dams and large dams (those with a normal storage capacity of 5000 acre-feet or more) built in each decade was generated from the data published by National Atlas of the United States. The analysis showed that the majority of the dams in Mississippi River Basin were built in 1960s and 1970s, but the majority of the large dams were built before the 1950s. These spatial and temporal changes in land use, land cover and hydrological modifications are linked to sediment, nutrient and environmental change of the basin.

  14. Late Cenozoic Basins of northern California

    NASA Astrophysics Data System (ADS)

    Nilsen, Tor H.; Clarke, Samuel H.

    1989-12-01

    The late Cenozoic basins of northern California developed in response to both convergent tectonics associated with subduction of the Farallon plate (and its modern representatives, the Juan de Fuca and Gorda plates) and transform tectonics associated with northward migration of the Mendocino triple junction and formation of the San Andreas fault system. The modern Eel River basin north of the Mendocino triple junction is an active forearc basin located between the Cascade magmatic arc to the east and the trench at the foot of the continental slope of northern California and Oregon to the west. Five different types of late Cenozoic basins or fragments of basins are preserved in onshore northern California south of the Mendocino triple junction: (1) remnants of a formerly more extensive Neogene forearc basin preserved locally in downdropped blocks within the San Andreas fault system; (2) remnants of slightly older trench-slope basins that generally developed west of but which may locally structurally underlie the younger forearc basin; (3) younger strike-slip-related structural basins that have developed along active right-lateral faults of the San Andreas fault system; (4) broad shallow embayments, perhaps similar to the modern San Francisco Bay, that were connected to the deeper Pacific Ocean to the west; and (5) structurally emplaced remnants of oceanic crust and its overlying sedimentary cover (the King Range terrane). Our preliminary stratigraphic and sedimentologic studies suggest that much of northern California was covered during the Neogene by a forearc basin that may have extended as far south as the San Francisco Bay region and into the southern San Joaquin Valley. As the Mendocino triple junction migrated northward during the late Cenozoic, the southern margin of the forearc basin was uplifted, basin deposits were stripped off by erosion, and the locus of forearc sedimentation shifted progressively northward through time. Preserved but isolated fragments

  15. Geology of interior cratonic sag basins

    SciTech Connect

    Leighton, M.W.; Eidel, J.J.; Kolata, D.R.; Oltz, D.F. )

    1990-05-01

    Interior cratonic sag basins are thick accumulations of sediment, generally more or less oval in shape, located entirely in the interiors of continental masses. Some are single-cycle basins and others are characterized by repeated sag cycles or are complex polyhistory basins. Many appear to have developed over ancient rift systems. Interior cratonic sag basins are typified by a dominance of flexural over fault-controlled subsidence, and a low ratio of sediment volume to surface area of the basin. The Baltic, Carpentaria, Illinois, Michigan, Parana, Paris, and Williston basins are examples of interior cratonic sag basins. Tectonics played a dominant role in controlling the shapes and the geometries of the juxtaposed packets of sedimentary sequences. While the mechanics of tectonic control are not clear, evidence suggests that the movements are apparently related to convergence of lithospheric plates and collision and breakup of continents. Whatever the cause, tectonic movements controlled the freeboard of continents, altering base level and initiating new tectono-sedimentologic regimes. Sag basins situated in low latitudes during their development commonly were sites of thick carbonates (e.g., Illinois, Michigan, Williston, and Paris basins). In contrast, siliciclastic sedimentation characterized basins that formed in higher latitudes (e.g., Parana and Carpentaria basins). Highly productive sag basins are characterized by widespread, mature, organic-rich source rocks, large structures, and good seals. Nonproductive basins have one or more of the following characteristics: immature source rocks, leaky plumbing, freshwater flushing, and/or complex geology due to numerous intrusions that inhibit mapping of plays.

  16. Basin stability in delayed dynamics

    PubMed Central

    Leng, Siyang; Lin, Wei; Kurths, Jürgen

    2016-01-01

    Basin stability (BS) is a universal concept for complex systems studies, which focuses on the volume of the basin of attraction instead of the traditional linearization-based approach. It has a lot of applications in real-world systems especially in dynamical systems with a phenomenon of multi-stability, which is even more ubiquitous in delayed dynamics such as the firing neurons, the climatological processes, and the power grids. Due to the infinite dimensional property of the space for the initial values, how to properly define the basin’s volume for delayed dynamics remains a fundamental problem. We propose here a technique which projects the infinite dimensional initial state space to a finite-dimensional Euclidean space by expanding the initial function along with different orthogonal or nonorthogonal basis. A generalized concept of basin’s volume in delayed dynamics and a highly practicable calculating algorithm with a cross-validation procedure are provided to numerically estimate the basin of attraction in delayed dynamics. We show potential applicabilities of this approach by applying it to study several representative systems of biological or/and physical significance, including the delayed Hopfield neuronal model with multistability and delayed complex networks with synchronization dynamics. PMID:26907568

  17. K West Basin canister survey

    SciTech Connect

    Pitner, A.L.

    1998-08-26

    A survey was conducted of the K West Basin to determine the distribution of canister types that contain the irradiated N Reactor fuel. An underwater camera was used to conduct the survey during June 1998, and the results were recorded on videotape. A full row-by-row survey of the entire basin was performed, with the distinction between aluminum and stainless steel Mark 1 canisters made by the presence or absence of steel rings on the canister trunions (aluminum canisters have the steel rings). The results of the survey are presented in tables and figures. Grid maps of the three bays show the canister lid ID number and the canister type in each location that contained fuel. The following abbreviations are used in the grid maps for canister type designation: IA = Mark 1 aluminum, IS = Mark 1 stainless steel, and 2 = Mark 2 stainless steel. An overall summary of the canister distribution survey is presented in Table 1. The total number of canisters found to contain fuel was 3842, with 20% being Mark 1 Al, 25% being Mark 1 SS, and 55% being Mark 2 SS. The aluminum canisters were predominantly located in the East and West bays of the basin.

  18. Great Basin geoscience data base

    USGS Publications Warehouse

    Raines, Gary L.; Sawatzky, Don L.; Connors, Katherine A.

    1996-01-01

    This CD-ROM serves as the archive for 73 digital GIS data set for the Great Basin. The data sets cover Nevada, eastern California, southeastern Oregon, southern Idaho, and western Utah. Some of the data sets are incomplete for the total area. On the CD-ROM, the data are provided in three formats, a prototype Federal Data Exchange standard format, the ESRI PC ARCVIEW1 format for viewing the data, and the ESRI ARC/INFO export format. Extensive documentation is provided to describe the data, the sources, and data enhancements. The following data are provided. One group of coverages comes primarily from 1:2,000,000-scale National Atlas data and can be assembled for use as base maps. These various forms of topographic information. In addition, public land system data sets are provided from the 1:2,500,000-scale Geologic Map of the United States and 1:500,000-scale geologic maps of Nevada, Oregon, and Utah. Geochemical data from the National Uranium Resource Evaluation (NURE) program are provided for most of the Great Basin. Geophysical data are provided for most of the Great Basin, typically gridded data with a spacing of 1 km. The geophysical data sets include aeromagnetics, gravity, radiometric data, and several derivative products. The thematic data sets include geochronology, calderas, pluvial lakes, tectonic extension domains, distribution of pre-Cenozoic terranes, limonite anomalies, Landsat linear features, mineral sites, and Bureau of Land Management exploration and mining permits.

  19. Basin stability in delayed dynamics

    NASA Astrophysics Data System (ADS)

    Leng, Siyang; Lin, Wei; Kurths, Jürgen

    2016-02-01

    Basin stability (BS) is a universal concept for complex systems studies, which focuses on the volume of the basin of attraction instead of the traditional linearization-based approach. It has a lot of applications in real-world systems especially in dynamical systems with a phenomenon of multi-stability, which is even more ubiquitous in delayed dynamics such as the firing neurons, the climatological processes, and the power grids. Due to the infinite dimensional property of the space for the initial values, how to properly define the basin’s volume for delayed dynamics remains a fundamental problem. We propose here a technique which projects the infinite dimensional initial state space to a finite-dimensional Euclidean space by expanding the initial function along with different orthogonal or nonorthogonal basis. A generalized concept of basin’s volume in delayed dynamics and a highly practicable calculating algorithm with a cross-validation procedure are provided to numerically estimate the basin of attraction in delayed dynamics. We show potential applicabilities of this approach by applying it to study several representative systems of biological or/and physical significance, including the delayed Hopfield neuronal model with multistability and delayed complex networks with synchronization dynamics.

  20. Biogeochemistry of a Suburban Basin

    NASA Astrophysics Data System (ADS)

    McDowell, W. H.; Daley, M. L.; Blumberg, J.

    2002-12-01

    A long-term research effort was recently established in the Lamprey River basin in southeastern New Hampshire. The watershed is largely forested, and has significant amounts of wetlands due to the relatively low topographic relief. Human population growth is rapid, resulting in conversion of forest and agricultural land to housing tracts. The primary focus of the project will be to examine the relationships between land use, land cover and water quality as the watershed continues to increase in population density. A secondary emphasis will be to examine the interactions between hydrologic flow paths, climatic variability, and biogeochemical processes that drive groundwater and surface water quality in the basin. Our initial work has quantified landscape attributes and related them to water quality. Results to date show that small tributary streams are relatively high in nitrogen relative to the main stem of the Lamprey; that human population density drives nitrate concentrations in the basin; and that DOC flux is predicted well by the model of Aitkenhead and McDowell that links DOC flux to watershed C:N ratio.

  1. The geologic history of Margaritifer basin, Mars

    USGS Publications Warehouse

    Salvatore, M. R.; Kraft, M. D.; Edwards, Christopher; Christensen, P.R.

    2016-01-01

    In this study, we investigate the fluvial, sedimentary, and volcanic history of Margaritifer basin and the Uzboi-Ladon-Morava (ULM) outflow channel system. This network of valleys and basins spans more than 8000 km in length, linking the fluvially dissected southern highlands and Argyre Basin with the northern lowlands via Ares Vallis. Compositionally, thermophysically, and morphologically distinct geologic units are identified and are used to place critical relative stratigraphic constraints on the timing of geologic processes in Margaritifer basin. Our analyses show that fluvial activity was separated in time by significant episodes of geologic activity, including the widespread volcanic resurfacing of Margaritifer basin and the formation of chaos terrain. The most recent fluvial activity within Margaritifer basin appears to terminate at a region of chaos terrain, suggesting possible communication between surface and subsurface water reservoirs. We conclude with a discussion of the implications of these observations on our current knowledge of Martian hydrologic evolution in this important region.

  2. The geologic history of Margaritifer basin, Mars

    NASA Astrophysics Data System (ADS)

    Salvatore, M. R.; Kraft, M. D.; Edwards, C. S.; Christensen, P. R.

    2016-03-01

    In this study, we investigate the fluvial, sedimentary, and volcanic history of Margaritifer basin and the Uzboi-Ladon-Morava outflow channel system. This network of valleys and basins spans more than 8000 km in length, linking the fluvially dissected southern highlands and Argyre basin with the northern lowlands via Ares Vallis. Compositionally, thermophysically, and morphologically distinct geologic units are identified and are used to place critical relative stratigraphic constraints on the timing of geologic processes in Margaritifer basin. Our analyses show that fluvial activity was separated in time by significant episodes of geologic activity, including the widespread volcanic resurfacing of Margaritifer basin and the formation of chaos terrain. The most recent fluvial activity within Margaritifer basin appears to terminate at a region of chaos terrain, suggesting possible communication between surface and subsurface water reservoirs. We conclude with a discussion of the implications of these observations on our current knowledge of Martian hydrologic evolution in this important region.

  3. Predicting the impact of future land-use and climate change on the groundwater system, Kleine Nete basin, Belgium

    NASA Astrophysics Data System (ADS)

    Salvadore, E.; Dams, J.; Batelaan, O.; van Daele, T.

    2009-04-01

    Climate changes including global warming and changing air circulation patterns, along with anthropogenic land-cover/use changes are expected to significantly influence both surface and subsurface hydrology in the near future. To be able to plan mitigating or adaptive actions, there is a strong need to study the individual as well as the combined impact of climate and land-use changes on the river, groundwater and wetland system on regional and local scale. Wetlands are particularly sensitive to modifications in water conditions; therefore this study focuses on modeling the impact of climate and land-use changes on the groundwater system and how this will further affect the distribution of most highly valued vegetation types. The regional study area, the Kleine Nete basin, comprises 580 km2 and is located 65 km north-east of Brussels. Within the basin, the 5.4 km2 sized groundwater dependent nature reserve "Olensbroek" serves as local study area. Initially, the "current" condition of the groundwater system has been determined; meteorological and land-use data for the period 1960-1990 has been used as input in a transient fully distributed physically-based hydrological model, WetSpa, to calculate with a daily time step the two weekly groundwater recharge. These values have been used as input for a transient MODFLOW model of the basin to simulate the groundwater level, flow, drainage and to derive other ecohydrological relevant parameters as mean lowest and highest groundwater level in every location of the basin. Future meteorological conditions have been obtained from a joint collaboration between the Royal Meteorological Institute of Belgium, K.U. Leuven Hydraulics Laboratory and the Royal Meteorological Institute of the Netherlands. All scenarios are based on the hierarchy of Global Climate Model (GCM) output, high resolution nested Regional Climate Model (RCM) simulations, and empirical/statistical downscaling using local observations. Choosing the change in

  4. Gravity Anomalies of the Lunar Orientale Basin and the Mercurian Caloris Basin

    NASA Astrophysics Data System (ADS)

    Blair, D. M.; Johnson, B. C.; Freed, A. M.; Melosh, H. J.

    2013-08-01

    We model the formation and evolution of the lunar Orientale and mercurian Caloris basin gravity anomalies using a combination of hydrocode and finite-element methods, constrained by free-air and Bouguer gravity anomalies and basin topography.

  5. Thermal evolution of sedimentary basins in Alaska

    USGS Publications Warehouse

    Johnsson, Mark J.; Howell, D.G.

    1996-01-01

    The complex tectonic collage of Alaska is reflected in the conjunction of rocks of widely varying thermal maturity. Indicators of the level of thermal maturity of rocks exposed at the surface, such as vitrinite reflectance and conodont color alteration index, can help constrain the tectonic evolution of such complex regions and, when combined with petrographic, modern heat flow, thermogeochronologic, and isotopic data, allow for the detailed evaluation of a region?s burial and uplift history. We have collected and assembled nearly 10,000 vitrinite-reflectance and conodont-color-alteration index values from the literature, previous U.S. Geological Survey investigations, and our own studies in Alaska. This database allows for the first synthesis of thermal maturity on a broadly regional scale. Post-accretionary sedimentary basins in Alaska show wide variability in terms of thermal maturity. The Tertiary interior basins, as well as some of the forearc and backarc basins associated with the Aleutian Arc, are presently at their greatest depth of burial, with immature rocks exposed at the surface. Other basins, such as some backarc basins on the Alaska Peninsula, show higher thermal maturities, indicating modest uplift, perhaps in conjunction with higher geothermal gradients related to the arc itself. Cretaceous ?flysch? basins, such as the Yukon-Koyukuk basin, are at much higher thermal maturity, reflecting great amounts of uplift perhaps associated with compressional regimes generated through terrane accretion. Many sedimentary basins in Alaska, such as the Yukon-Koyukuk and Colville basins, show higher thermal maturity at basin margins, perhaps reflecting greater uplift of the margins in response to isostatic unloading, owing to erosion of the hinterland adjacent to the basin or to compressional stresses adjacent to basin margins.

  6. Reserve estimates in western basins. Part 2: Piceance Basin

    SciTech Connect

    1995-10-01

    This study characterizes an extremely large gas resource located in low permeability, sandstone reservoirs of the Mesaverde group in the Piceance Basin, Colorado. Total in place resource is estimated at 307.3 Tcf. Via application of geologic, engineering and economic criteria, the portion of this resource potentially recoverable as reserves is estimated. Those volumes estimated include probable, possible and potential categories and total 5.8 Tcf as a mean estimate of recoverable gas for all plays considered in the basin. About 82.6% of the total evaluated resource is contained within sandstones that have extremely poor reservoir properties with permeabilities considered too low for commerciality using current frac technology. Cost reductions and technology improvements will be required to unlock portions of this enormous resource. Approximately 2.7% of the total resource is contained within sandstone reservoirs which do not respond to massive hydraulic fracture treatments, probably due to their natural lenticular nature. Approximately 6.8% of the total resource is located in deeply buried settings below deepest established production. Approximately 7.9% of the total resource is considered to represent tight reservoirs that may be commercially exploited using today`s hydraulic fracturing technology. Recent technology advances in hydraulic fracturing practices in the Piceance Basin Mesaverde has resulted in a marked improvement in per well gas recovery which, where demonstrated, has been incorporated into the estimates provided in this report. This improvement is so significant in changing the risk-reward relationship that has historically characterized this play, that previously uneconomic areas and resources will graduate to the economically exploitable category. 48 refs., 96 figs., 18 tabs.

  7. Reserves in western basins: Part 1, Greater Green River basin

    SciTech Connect

    Not Available

    1993-10-01

    This study characterizes an extremely large gas resource located in low permeability, overpressured sandstone reservoirs located below 8,000 feet drill depth in the Greater Green River basin, Wyoming. Total in place resource is estimated at 1,968 Tcf. Via application of geologic, engineering and economic criteria, the portion of this resource potentially recoverable as reserves is estimated. Those volumes estimated include probable, possible and potential categories and total 33 Tcf as a mean estimate of recoverable gas for all plays considered in the basin. Five plays (formations) were included in this study and each was separately analyzed in terms of its overpressured, tight gas resource, established productive characteristics and future reserves potential based on a constant $2/Mcf wellhead gas price scenario. A scheme has been developed to break the overall resource estimate down into components that can be considered as differing technical and economic challenges that must be overcome in order to exploit such resources: in other words, to convert those resources to economically recoverable reserves. Total recoverable reserves estimates of 33 Tcf do not include the existing production from overpressured tight reservoirs in the basin. These have estimated ultimate recovery of approximately 1.6 Tcf, or a per well average recovery of 2.3 Bcf. Due to the fact that considerable pay thicknesses can be present, wells can be economic despite limited drainage areas. It is typical for significant bypassed gas to be present at inter-well locations because drainage areas are commonly less than regulatory well spacing requirements.

  8. Petroleum system of the Gippsland Basin, Australia

    USGS Publications Warehouse

    Bishop, Michele G.

    2000-01-01

    The Gippsland Basin Province 3930, located on the southeastern coast of Australia, is formed from two successive failed rifts that developed into a passive margin during the Cretaceous. Formation of this basin is related to the break up of Gondwana, which resulted in the separation of Antarctica from Australia, and the separation of the New Zealand and Lord Howe Rise continental crust from Australia. Coals and coaly shales of Late Cretaceous through Eocene age are the source rocks for oil and gas that accumulated predominantly in anticlinal traps. The basin was Australia?s major producing basin until 1996 when daily oil/condensate production from the North West Shelf surpassed it.

  9. Late Paleozoic structural evolution of Permian basin

    SciTech Connect

    Ewing, T.E.

    1984-04-01

    The southern Permian basin is underlain by the NNW-trending Central Basin disturbed belt of Wolfcamp age (Lower Permian), the deep Delaware basin to its west, and the shallower Midland basin to its eat. The disturbed belt is highly segmented with zones of left-lateral offset. Major segments from south to north are: the Puckett-Grey Ranch zone; the Fort Stockton uplift; the Monahans transverse zone; the Andector ridges and the Eunice ridge; the Hobbs transverse zone; and the Tatum ridges, which abut the broad Roosevelt uplift to the north. The disturbed belt may have originated along rift zones of either Precambrian or Cambrian age. The extent of Lower and Middle Pennsylvanian deformation is unclear; much of the Val Verde basin-Ozona arch structure may have formed then. The main Wolfcamp deformation over thrust the West Texas crustal block against the Delaware block, with local denudation of the uplifted edge and eastward-directed backthrusting into the Midland basin. Latter in the Permian, the area was the center of a subcontinental bowl of subsidence - the Permian basin proper. The disturbed belt formed a pedestal for the carbonate accumulations which created the Central Basin platform. The major pre-Permian reservoirs of the Permian basin lie in large structural and unconformity-bounded traps on uplift ridges and domes. Further work on the regional structural style may help to predict fracture trends, to assess the timing of oil migration, and to evaluate intrareservoir variations in the overlying Permian giant oil fields.

  10. Petroleum geology of Norton basin, Alaska

    SciTech Connect

    Fisher, M.A.

    1982-03-01

    Basement rocks beneath the main part of the Norton basin were deformed and heated during the Late Jurassic and Early Cretaceous to the extent that these rocks were not capable of generating hydrocarbons when the basin formed during the latest Cretaceous or early Paleogene. Consequently, source rocks for oil, if they exist, are most likely to be within the basin fill. If the Norton basin began to form 65 m.y. ago, subsided at a nearly constant rate, and had an average geothermal gradient of between 35 and 45/sup 0/C/km, then rocks as young as late Oligocene are in the oil window (vitrinite reflectance between 0.65 and 1.30%). The appearance on seismic sections of reflections from rocks in and below the calculated oil window suggests that these rocks were deposited in a nonmarine environment. Thus, gas and condensate are the most likely hydrocarbons to be present in the basin. Because of their shallow depth of burial, Neogene (possibly marine) rocks are not likely to be thermally mature anywhere in the basin. Deep parts of the basin formed as isolated faultbounded lows; consequently, the volume of mature rocks makes up at most 11% of the total basin fill. Numerous potential traps for hydrocarbons exist in the Norton basin; the traps include fractured or weathered basement rocks in horsts, strata in alluvial fans on the flanks of horsts, and arched strata over horsts.

  11. Basin Management under the Global Climate Change (Take North-East Asia Heilongjiang -Amur Basin and Taihu Basin For Example)

    NASA Astrophysics Data System (ADS)

    Liu, S.; Zhou, Z.; Zhong, G.; Zhang, X.

    2015-12-01

    The impact of global climate change on environment and society causes increasingly concern in different countries around the world. The main climate characteristic values, such as precipitation and temperature, have been changed, which leads to the variation of water resources, especially in large basins. Heilongjiang-Amur Basin and Taihu Basin are two large and important basins in China with large area and population. As global climate change and human activities have siganificant impacts on hydrology and water resources in two basins, the analysis of climate change are of great value. In this study, in Heilongjiang-Amur Basin, precipitation and temperature are investigated and their variation are predicted. And in Taihu Basin, precipitation including plum rain and typhoon, are studied and the variation trend of precipitation is predicted. Hence, the impacts of global climate change are assessed. From the result, it shows that the average temperature will continue to increase, and the precipitation will reduce first and then turn to increase in these two basins. It demonstrates that the water resources have been affected a lot by climate change as well as human activities. And these conclusions are provided as reference for policy makers and basin authorities in water resources management and natural hazards mitigation. Meanwhile, according to basins' particualr characters, the suggestions to future water resources management in two basins are given, and more scientific, comprehensive and sustained managements are required. Especially, in Heilongjiang-Amur River, which is a boundary river between China and Russia, it is very essential to enhance the cooperation between two countries.

  12. Regional geophysics and the basement of cratonic basins: a comparative study with the Michigan basin

    SciTech Connect

    Hinze, W.J.; Lidiak, E.G.

    1986-08-01

    The basement of the Michigan basin consists of four major provinces - the complex metasedimentary, metavolcanic, and igneous rocks of the Penokean orogenic assemblage in the north, the felsic anorogenic igneous rocks to the south, the highly metamorphosed schists, gneisses, and related igneous intrusions of the Grenville province in the east, and a middle Proterozoic rift zone, which transects the basin from the north to the southeast margin. Sparse basement drill holes and characteristic geophysical patterns support this interpretation. The direct geologic information on the basement of other cratonic basins is not as well known. However, regional geophysical surveys and sparse, poorly distributed basement drill holes provide information on the complex character and structural relationships of the basement of other basins. Like the Michigan basin, many cratonic basins (e.g., Illinois, Williston, and Paris basins) are underlain by dense and commonly more magnetic rocks than adjacent areas. As in the Michigan basin, these rocks are interpreted to have a profound effect on the origin and tectonic development of the basins. Geologic and geophysical evidence indicates that many of these dense basement rocks originated in rifts that formed hundreds of millions of years prior to basin development. A comparison of the basement in cratonic basins provides important constraints on the origin and tectonic development of the Michigan basin.

  13. 75 FR 43915 - Basin Electric Power Cooperative: Deer Creek Station

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-27

    ... Rural Utilities Service Basin Electric Power Cooperative: Deer Creek Station AGENCY: Rural Utilities... CFR Part 1794), and the Western Area Power Administration's (Western) NEPA implementing regulations... environmental impacts of and alternatives to Basin Electric Power Cooperative's (Basin Electric) application...

  14. Free energy basin-hopping

    NASA Astrophysics Data System (ADS)

    Sutherland-Cash, K. H.; Wales, D. J.; Chakrabarti, D.

    2015-04-01

    A global optimisation scheme is presented using basin-hopping with the acceptance criterion based on approximate free energy for the corresponding local minima of the potential energy. The method is illustrated for atomic and colloidal clusters and peptides to examine how the predicted global free energy minimum changes with temperature. Using estimates for the local free energies based on harmonic vibrational densities of states provides a computationally effective framework for predicting trends in structure at finite temperature. The resulting scheme represents a powerful tool for exploration of energy landscapes throughout molecular science.

  15. Central Nebraska river basins Nebraska

    USGS Publications Warehouse

    Huntzinger, Thomas L.; Ellis, Michael J.

    1993-01-01

    The Central Nebraska Basins (NAWQA) study unit includes the Platte River and two major tributaries, the Loup and Elkhorn Rivers. Platte River flows are variable of diversions, but the Loup and Elkhorn Rivers originate in an area of dune sand covered by grassland that generates consistent base flows. Ground water has no regional confining units and the system is a water table aquifer throughout. Macroinvertebrate and fish taxa were related to stream flow. One of the four wetland complexes includes habitat for threatened and endangered bird species. A water quality assessments will be based on the differences in environmental setting in each of four subunits within the study unit.

  16. Seismic Characterization of the Jakarta Basin

    NASA Astrophysics Data System (ADS)

    Cipta, A.; Saygin, E.; Cummins, P. R.; Masturyono, M.; Rudyanto, A.; Irsyam, M.

    2015-12-01

    Jakarta, Indonesia, is home to more than 10 million people. Many of these people live in seismically non-resilient structures in an area that historical records suggest is prone to earthquake shaking. The city lies in a sedimentary basin composed of Quaternary alluvium that experiences rapid subsidence (26 cm/year) due to groundwater extraction. Forecasts of how much subsidence may occur in the future are dependent on the thickness of the basin. However, basin geometry and sediment thickness are poorly known. In term of seismic hazard, thick loose sediment can lead to high amplification of seismic waves, of the kind that led to widespread damage in Mexico city during the Michoacan Earthquake of 1985. In order to characterize basin structure, a temporary seismograph deployment was undertaken in Jakarta in Oct 2013- Jan 2014. A total of 96 seismic instrument were deployed throughout Jakarta were deployed throughout Jakarta at 3-5 km spacing. Ambient noise tomography was applied to obtain models of the subsurface velocity structure. Important key, low velocity anomalies at short period (<8s) correspond to the main sedimentary sub-basins thought to be present based on geological interpretations of shallow stratigraphy in the Jakarta Basin. The result shows that at a depth of 300 m, shear-wave velocity in the northern part (600 m/s) of the basin is lower than that in the southern part. The most prominent low velocity structure appears in the northwest of the basin, down to a depth of 800 m, with velocity as low as 1200 m/s. This very low velocity indicates the thickness of sediment and the variability of basin geometry. Waveform computation using SPECFEM2D shows that amplification due to basin geometry occurs at the basin edge and the thick sediment leads to amplification at the basin center. Computation also shows the longer shaking duration occurrs at the basin edge and center of the basin. The nest step will be validating the basin model using earthquake events

  17. Basin-scale relations via conditioning

    USGS Publications Warehouse

    Troutman, B.M.; Karlinger, M.R.; Guertin, D.P.

    1989-01-01

    A rainfall-runoff model is used in conjunction with a probabilistic description of the input to this model to obtain simple regression-like relations for basin runoff in terms of basin and storm characteristics. These relations, similar to those sought in regionalization studies, are computed by evaluating the conditional distribution of model output given basin and storm characteristics. This method of conditioning provides a general way of examining model sensitivity to various components of model input. The resulting relations may be expected to resemble corresponding relations obtained by regionalization using actual runoff to the extent that the rainfall-runoff model and the model input specification are physically realistic. The probabilistic description of model input is an extension of so-called "random-model" of channel networks and involves postulating an ensemble of basins and associated probability distributions that mimic the variability of basin characteristics seen in nature. Application is made to small basins in the State of Wyoming. Parameters of the input variable distribution are estimated using data from Wyoming, and basin-scale relations are estimated both, parametrically and nonparametrically using model-generated runoff from simulated basins. Resulting basin-scale relations involving annual flood quantiles are in reasonable agreement with those presented in a previous regionalization study, but error estimates are smaller than those in the previous study, an artifact of the simplicity of the rainfall-runoff model used in this paper. We also obtain relations for peak of the instantaneous unit hydrograph which agree fairly well with theoretical relations given in the literature. Finally, we explore the issues of sensitivity of basin-scale, relations and error estimates to parameterization of the model input probability distribution and of how this sensitivity is related to making inferences about a particular ungaged basin. ?? 1989 Springer-Verlag.

  18. Cenozoic evolution of San Joaquin basin, California

    SciTech Connect

    Bartow, J.A.

    1988-03-01

    The Neogene San Joaquin basin in the southern part of the 700-km long Great Valley of California is a successor to a late Mesozoic and earliest Tertiary forearc basin. The transition from forearc basin to the more restricted Neogene marine basin occurred principally during the Paleogene as the plate tectonic setting changed from oblique convergence to normal convergence, and finally to the initiation of tangential (transform) movement near the end of the Oligocene. Regional-scale tectonic events that affected the basin include: (1) clockwise rotation of the southernmost Sierra Nevada, and large-scale en echelon folding in the southern Diablo Range, both perhaps related to Late Cretaceous and early Tertiary right slip on the proto-San-Andreas fault; (2) regional uplift of southern California in the Oligocene that resulted from the subduction of the Pacific-Farallon spreading ridge: (3) extensional tectonism in the Basin and Range province, particularly in the Miocene; (4) wrench tectonism adjacent to the San Andreas fault in the Neogene; (5) northeastward emplacement of a wedge of the Franciscan complex at the west side of the Sierran block, with associated deep-seated thrusting in the late Cenozoic; and (6) the accelerated uplift of the Sierra Nevada beginning in the late Miocene. Neogene basin history was controlled principally by the tectonic effects of the northwestward migration of the Mendocino triple junction along the California continental margin and by the subsequent wrench tectonism associated with the San Andreas fault system. East-west compression in the basin, resulting from extension in the Basin and Range province was an important contributing factor to crustal shortening at the west side of the valley. Analysis of the sedimentary history of the basin, which was controlled to some extent by eustatic sea level change, enables reconstruction of the basin paleogeography through the Cenozoic.

  19. Potential for a basin-centered gas accumulation in the Albuquerque Basin, New Mexico

    USGS Publications Warehouse

    Johnson, Ronald C.; Finn, Thomsa M.; Nuccio, Vito F.

    2001-01-01

    The potential that a basin-centered or continuous-type gas accumulation is present in the Albuquerque Basin in central New Mexico was investigated. The Albuquerque Basin is one of the many rift basins that make up the Rio Grand rift system, an area of active extension from Oligocene to recent time. The basin is significantly different from other Rocky Mountain basins that contain basin-centered gas accumulations because it is actively subsiding and is at near maximum burial and heating conditions at the present time. Burial reconstructions suggest that Cretaceous-age source rocks began to generate gas in the deeper parts of the basin about 20 million years ago and are still generating large amounts of gas. The high mud weights typically used while drilling the Cretaceous interval in the deeper areas of the basin suggest some degree of over-pressuring. Gas shows are commonly reported while drilling through the Cretaceous interval; however, attempts to complete gas wells in the Cretaceous have resulted in subeconomic quantities of gas, primarily because of low permeabilities. Little water has been reported. All of these characteristics suggest that a basin-centered gas accumulation of some sort is present in the Albuquerque Basin.

  20. 33 CFR 401.48 - Turning basins.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... 1—Opposite Brossard. (b) Turning Basin No. 2—Between Lock 7 and the Guard Gate Cut for vessels up to... vessels up to 107 m in overall length. (b) Turning Basin No. 2—Between Lock 7 and the Guard Gate Cut...

  1. 33 CFR 401.48 - Turning basins.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... 1—Opposite Brossard. (b) Turning Basin No. 2—Between Lock 7 and the Guard Gate Cut for vessels up to... vessels up to 107 m in overall length. (b) Turning Basin No. 2—Between Lock 7 and the Guard Gate Cut...

  2. 33 CFR 401.48 - Turning basins.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... 1—Opposite Brossard. (b) Turning Basin No. 2—Between Lock 7 and the Guard Gate Cut for vessels up to... vessels up to 107 m in overall length. (b) Turning Basin No. 2—Between Lock 7 and the Guard Gate Cut...

  3. African sedimentary basins - Tectonic controls on prospectivity

    SciTech Connect

    Bunter, M.A.G.; Crossley, R.; Hammill, M.; Jones, P.W.; Morgan, R.K.; Needham, D.T.; Spaargaren, F.A. )

    1991-03-01

    An important prerequisite for the evaluation of any sedimentary basin is the understanding of its regional tectonic setting. This is especially so in the underexplored regions of Africa. The majority of African sedimentary basins developed in an extensional setting although some have undergone subsequent compressional or transpressional deformation. The geometry and evolution of these basins is often influenced by basement structure. The extensional phase of basin development controls not only the distribution of syn-rift sediments but also the magnitude of post-rift regional subsidence and the preservation or removal of pre-rift sediments. This has important consequences for exploration models of syn-rift and pre-rift source rocks and reservoirs. Post-rift basin inversion and uplift provide crucial controls on the preservation of mature source rocks and quality of reservoirs. The distribution, nature, timing, and possible mechanisms of this uplift in Africa will be addressed. The hydrocarbon prospectivity of African basis appears to be highly variable although the limited exploration of some regions makes the exact extent of this variability unclear. Basins considered potentially prospective range from late Precambrian to Tertiary in age. The various tectonic controls outlined above, and criteria for the evaluation of underexplored areas, will be demonstrated by reference to basins studied by The Robertson Group. Examples described include basins from Bagon, Angola, Namibia, East Africa, Tertiary Rift and Karoo Rifts, and North Africa (Sudan, Egypt, Algeria, and Morocco).

  4. Frontier sedimentary basins of New Zealand region

    SciTech Connect

    Beggs, J.M. )

    1991-03-01

    Petroleum-prospective basins of New Zealand began to form by mid-Cretaceous rifting of crustal elements previously assembled at the Gondwana continental margin. During the latest Cretaceous-early Cenozoic New Zealand separated from Australia and Antarctica by sea-floor spreading. An overall transgression in widely recorded in this post-rift phase, with decreasing clastic sediment supply as land area and relief were reduced. Mid-Cenozoic initiation of the modern plate boundary has resulted in uplift of mountain ranges, subsidence and filling of troughs, progradation of the shelf, and common reactivation or eversion of older structures. Petroleum potential of less explored basins can be compared to the productive Taranki basin. Source rocks are coal-rich deposits of the rift phase, also developed in Great South, Canterbury/Chatham, Western Southland, West Coast, and Northland basins. A different source contributes to oil and gas seeps on the East Coast, a continental margin during Late Cretaceous. The main reservoirs of Taranaki are early Cenozoic coastal and fluvial sands, also present in Great South, Canterbury, and West Coast and possibly other basins. Other Taranaki reservoirs include mid-Cenozoic limestone and Miocene turbidites, which are widespread in most other basins. Pliocene limestones have excellent reservoir potential on the East Coast. Late Cenozoic tectonics, essential to trap development and significant for maturation in Taranaki, have created similar structures in basins near the plate boundary but are less significant in the development of Great South, eastern Canterbury/Chatham, and Northland basins.

  5. Relation between Tethys sea and Tarim basin

    SciTech Connect

    Wei Junchao )

    1988-08-01

    The Tarim basin is the largest continental basin in China. It is known as the heart of central Asia. Still it was related to the Mediterranean Sea in the geological past. Based on the investigations of paleontology, stratigraphy, tectonics, and remote sensing, it is suggested that Tethys and the Tarim basin should be connected from the Late Cretaceous to Miocene. The northern branch of the Tethys sea channel began to pass through the Alay gap and invade the Tarim basin at the beginning of the Late Cretaceous. Up to the Miocene, marine invasion and marine regression must have happened six times in the western Tarim basin. The Paleocene marine invasion encroached upon the widest area and lasted the longest of the six times, which extended to the region of the southern Hotan River. The occurrence of the Paleocene marine fossils in the Kuqa Seg indicates the influence of the marine invasion. At the end of the Miocene, seawater receded fully from the Tarim basin. A Miocene petroleum field has been found in the Yecheng Seg of the western Tarim basin. According to the relationship between Tethys and the Tarim basin, the potentialities of the Late Cretaceous-Miocene hydrocarbon source are considered to be great.

  6. Water quality in the eastern Iowa basins

    USGS Publications Warehouse

    Kalkhoff, Stephen J.; Barnes, Kymm K.; Becher, Kent D.; Savoca, Mark E.; Schnoebelen, Douglas J.; Sadorf, Eric M.; Porter, Stephen D.; Sullivan, Daniel J.; Creswell, John

    2001-01-01

    The Eastern Iowa Basins Study Unit includes the Wapsipinicon, Cedar, Iowa, and Skunk River basins and covers approximately 19,500 square miles in eastern Iowa and southern Minnesota. More than 90 percent of the land in the study unit is used for agricultural purposes. Forested areas account for only 4 percent of the land area.

  7. Scientific review of great basin wildfire issues

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The University Nevada Reno, College of Agriculture and Resource Concepts Inc., co-sponsored a Great Basin Wildfire Forum in September 2007 to address a “Scientific Review of the Ecological and Management History of Great Basin Natural Resources and Recommendations to Achieve Ecosystem Restoration”. ...

  8. Scientific Review of Great Basin Wildfire Issues

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The University Nevada Reno, College of Agriculture and Resource Concepts Inc., co-sponsored a Great Basin Wildfire Forum in September 2007 to address a “Scientific Review of the Ecological and Management History of Great Basin Natural Resources and Recommendations to Achieve Ecosystem Restoration”. ...

  9. Wyoming Basin Rapid Ecoregional Assessment

    USGS Publications Warehouse

    Carr, Natasha B.; Means, Robert E.

    2013-01-01

    The overall goal of the Wyoming Basin Rapid Ecoregional Assessment (REA) is to provide information that supports regional planning and analysis for the management of ecological resources. The REA provides an assessment of baseline ecological conditions, an evaluation of current risks from drivers of ecosystem change (including energy development, fire, and invasive species), and a predictive capacity for evaluating future risks (including climate change). Additionally, the REA may be used for identifying priority areas for conservation or restoration and for assessing cumulative effects of multiple land uses. The Wyoming Basin REA will address Management Questions developed by the Bureau of Land Management and other agency partners for 8 major biomes and 19 species or species assemblages. The maps developed for addressing Management Questions will be integrated into overall maps of landscape-level ecological values and risks. The maps can be used to address the goals of the REA at a number of levels: for individual species, species assemblages, aquatic and terrestrial systems, and for the entire ecoregion. This allows flexibility in how the products of the REA are compiled to inform planning and management actions across a broad range of spatial scales.

  10. Basins in ARC-continental collisions

    USGS Publications Warehouse

    Draut, Amy E.; Clift, Peter D.; Busby, Cathy; Azor, Antonio

    2012-01-01

    Arc-continent collisions occur commonly in the plate-tectonic cycle and result in rapidly formed and rapidly collapsing orogens, often spanning just 5-15 My. Growth of continental masses through arc-continent collision is widely thought to be a major process governing the structural and geochemical evolution of the continental crust over geologic time. Collisions of intra-oceanic arcs with passive continental margins (a situation in which the arc, on the upper plate, faces the continent) involve a substantially different geometry than collisions of intra-oceanic arcs with active continental margins (a situation requiring more than one convergence zone and in which the arc, on the lower plate, backs into the continent), with variable preservation potential for basins in each case. Substantial differences also occur between trench and forearc evolution in tectonically erosive versus tectonically accreting margins, both before and after collision. We examine the evolution of trenches, trench-slope basins, forearc basins, intra-arc basins, and backarc basins during arc-continent collision. The preservation potential of trench-slope basins is low; in collision they are rapidly uplifted and eroded, and at erosive margins they are progressively destroyed by subduction erosion. Post-collisional preservation of trench sediment and trench-slope basins is biased toward margins that were tectonically accreting for a substantial length of time before collision. Forearc basins in erosive margins are usually floored by strong lithosphere and may survive collision with a passive margin, sometimes continuing sedimentation throughout collision and orogeny. The low flexural rigidity of intra-arc basins makes them deep and, if preserved, potentially long records of arc and collisional tectonism. Backarc basins, in contrast, are typically subducted and their sediment either lost or preserved only as fragments in melange sequences. A substantial proportion of the sediment derived from

  11. Rifting Thick Lithosphere - Canning Basin, Western Australia

    NASA Astrophysics Data System (ADS)

    Czarnota, Karol; White, Nicky

    2016-04-01

    The subsidence histories and architecture of most, but not all, rift basins are elegantly explained by extension of ~120 km thick lithosphere followed by thermal re-thickening of the lithospheric mantle to its pre-rift thickness. Although this well-established model underpins most basin analysis, it is unclear whether the model explains the subsidence of rift basins developed over substantially thick lithosphere (as imaged by seismic tomography beneath substantial portions of the continents). The Canning Basin of Western Australia is an example where a rift basin putatively overlies lithosphere ≥180 km thick, imaged using shear wave tomography. Subsidence modelling in this study shows that the entire subsidence history of the <300 km wide and <6 km thick western Canning Basin is adequately explained by mild Ordovician extension (β≈1.2) of ~120 km thick lithosphere followed by post-rift thermal subsidence. This is consistent with the established model, described above, albeit with perturbations due to transient dynamic topography support which are expressed as basin-wide unconformities. In contrast the <150 km wide and ~15 km thick Fitzroy Trough of the eastern Canning Basin reveals an almost continuous period of normal faulting between the Ordovician and Carboniferous (β<2.0) followed by negligible post-rift thermal subsidence. These features cannot be readily explained by the established model of rift basin development. We attribute the difference in basin architecture between the western and eastern Canning Basin to rifting of thick lithosphere beneath the eastern part, verified by the presence of ~20 Ma diamond-bearing lamproites intruded into the basin depocentre. In order to account for the observed subsidence, at standard crustal densities, the lithospheric mantle is required to be depleted in density by 50-70 kg m-3, which is in line with estimates derived from modelling rare-earth element concentrations of the ~20 Ma lamproites and global isostatic

  12. Petroleum geochemistry of the Zala Basin, Hungary

    USGS Publications Warehouse

    Clayton, J.L.; Koncz, I.

    1994-01-01

    The Zala basin is a subbasin within the Pannonian basin. Geochemical study of oils and rocks in the basin indicate that two, and possibly three, genetic oil types are present in the basin. Miocene source rocks, previously believed to be the predominant source rock, have expelled minor amounts of hydrocarbons. The main source rock is the Upper Triassic (Rhaetian) Kossen Marl Formation or its stratigraphic equivalent. Knowledge of the geochemical characteristics of oils derived from these Upper Triassic source rocks and understanding of the source rock distribution and maturation history are important for recognizing Triassic oil-source bed relationships and for further exploration in other basins in Hungary and other parts of Europe where Triassic source rocks are present. -from Authors

  13. China, JNOC start exploration in Tarim basin

    SciTech Connect

    Not Available

    1992-06-15

    This paper reports that a joint venture of China National Oil and Natural Gas Corp and Japan National Oil Corp (JNOC) has begun exploration in Northwest China's remote Tarim basin in Xinjiang Uygur Autonomous Region. That marks the first time China has allowed a foreign oil company to participate in exploration of the highly prospective basin. China pins much of its hope for the future on the Tarim basin as production declines from its older, mainstay fields in the east and offshore results have proved largely disappointing. The Chinese-Japanese combine began operations in the southwest part of the 560,000 sq km basin. The 200 member exploration team plans to complete a seismic survey covering 3,500 line km in the Kashi and Yecheng areas during the next 4 1/2 years. The survey follows a feasibility study that began last October covering 30,000 sq km in the basin.

  14. Determination of the Relationship between Hydrologic Processes and Basin Morphometry - The Lamos Basin (Mersin, Turkey)

    NASA Astrophysics Data System (ADS)

    Yıldırım, Ümit; Güler, Cüneyt

    2016-04-01

    This study has been carried out to determine the relationship between hydrologic processes and basin morphometry in the Lamos Basin, which is located at the northern part of the Mersin (SE Turkey). The morphometric parameters of the basin was derived from the 1:25K scale topographic map sheets that were digitized using ArcGIS 9.3.1 geographic information system (GIS) software. Morphometric parameters considered in this study include basin area, basin length, basin perimeter length, stream order, stream number, stream length, mean stream length, basin relief, drainage density, stream frequency, drainage texture, bifurcation ratio, form factor, elongation ratio, overland flow length, relief ratio, and hypsometric integral. The results have shown that there are 1252 individual stream reaches with a total length of 1414.1 km in the Lamos basin, which covers an area of 1358 km2 and has a length of 103 km in the N-S direction. Furthermore, the basin has a medium drainage density of 1.04 1/km with a stream frequency and drainage texture values of 0.92 and 4.33, respectively. The basin can be classified as elongated because of the low values of elongation ratio (0.48) and form factor (0.12). The hypsometric integral of the basin (0.58) indicates that it is in the youth period and thus reasonably sensitive to erosion. The values of drainage texture, drainage density, and stream frequency indicate that the Lamos basin is moderately well drained, therefore overland flow in the basin is not expected to be so quick. Thus, in case of occurrence of sudden peak flows, sensitivity to the land sliding and erosion may increase further. As a result, it is suggested that human activities in the basin should be limited in areas in fairly close proximity to the present day stream network to prevent or reduce the risk to life and property.

  15. Preliminary evaluation of nominal drainage basin volume as a potentially useful morphometric parameter for small mountain basins

    SciTech Connect

    Keaton, J.R.

    1985-01-01

    Morphometric basin parameters have been used in quantitative geomorphic assessments since Horton's Hydrophysical Approach in 1945. A relationship between basin form and dominant process in small mountain basins in the western United States would be valuable for use in differentiating basins which produce deep-seated landslides from those which produce debris flows from debris slides. Drainage basin volume seems like it should be a parameter directly related to the dominant process operating in a basin. Consequently, it may be a potentially useful morphometric parameter. Nominal drainage basin volume is herein defined as the volume creates by the basin topography and linear projection of topographic contours across the basin. Incremental volume is computed from area encompassed by topographic contours and projections and the contour interval using the formula for the volume of the frustrum of a cone. Seven basins in the Wasatch Range and five in the Wasatch Plateau of Utah show strong relationship of log Basin Area to log Basin Volume (r/sup 2/ = 0.97). The relationship between average Basin Slope and log Basin Volume was poorer (r/sup 2/ = 0.78) than between Basin Slope and log Basin Area (r/sup 2/ = 0.87). This suggests that basin area may be a more useful parameter than basin volume, especially since area is more easily measured.

  16. Rocky Mountain Tertiary coal-basin models and their applicability to some world basins

    USGS Publications Warehouse

    Flores, R.M.

    1989-01-01

    Tertiary intermontane basins in the Rocky Mountain region of the United States contain large amounts of coal resources. The first major type of Tertiary coal basin is closed and lake-dominated, either mud-rich (e.g., North Park Basin, Colorado) or mud plus carbonate (e.g., Medicine Lodge Basin, Montana), which are both infilled by deltas. The second major type of Tertiary coal basin is open and characterized by a preponderance of sediments that were deposited by flow-through fluvial systems (e.g., Raton Basin, Colorado and New Mexico, and Powder River Basin, Wyoming and Montana). The setting for the formation of these coals varies with the type of basin sedimentation, paleotectonism, and paleoclimate. The mud-rich lake-dominated closed basin (transpressional paleotectonism and warm, humid paleoclimate), where infilled by sandy "Gilbert-type" deltas, contains thick coals (low ash and low sulfur) formed in swamps of the prograding fluvial systems. The mud- and carbonate-rich lake-dominated closed basin is infilled by carbonate precipitates plus coarse-grained fan deltas and fine-grained deltas. Here, thin coals (high ash and high sulfur) formed in swamps of the fine-grained deltas. The coarse-clastic, open basins (compressional paleotectonism and warm, paratropical paleoclimate) associated with flow-through fluvial systems contain moderately to anomalously thick coals (high to low ash and low sulfur) formed in swamps developed in intermittently abandoned portions of the fluvial systems. These coal development patterns from the Tertiary Rocky Mountain basins, although occurring in completely different paleotectonic settings, are similar to that found in the Tertiary, Cretaceous, and Permian intermontane coal basins in China, New Zealand, and India. ?? 1989.

  17. Umatilla Basin Habitat Improvement Project.

    SciTech Connect

    Bailey, Timothy D.

    1990-01-01

    This annual report is in fulfillment of contract obligations with Bonneville Power Administration which is the funding source for the Oregon Department of Fish and Wildlife's Umatilla Basin Habitat Improvement Project. The major activities undertaken during this report period were: procurement of 17 cooperative lease agreements with private landowners, design and layout of 8.6 miles of riparian exclosure fence and 3.0 miles of instream structures, development of five fencing contracts and six instream work contracts. Results include implementation of 10 miles of fencing and 3 miles of instream work. Other activities undertaken during this report period are: data collection from 90 habitat monitoring transects, collection and summarization of temperature data, photopoint establishment, coordination with numerous agencies and tribes and education of all age groups on habitat improvement and protection. 4 refs., 4 figs., 6 tabs.

  18. Wyoming Basin Rapid Ecoregional Assessment

    USGS Publications Warehouse

    Carr, Natasha B.; Melcher, Cynthia P.

    2015-08-28

    We evaluated Management Questions (Core and Integrated) for each species and community for the Wyoming Basin REA. Core Management Questions address primary management issues, including (1) where is the Conservation Element, and what are its key ecological attributes (characteristics of species and communities that may affect their long-term persistence or viability); (2) what and where are the Change Agents; and (3) how do the Change Agents affect the key ecological attributes? Integrated Management Questions synthesize the Core Management Questions as follows: (1) where are the areas with high landscape-level ecological values; (2) where are the areas with high landscape-level risks; and (3) where are the potential areas for conservation, restoration, and development? The associated maps and key findings for each Management Question are summa

  19. Wyoming Basin Rapid Ecoregional Assessment

    USGS Publications Warehouse

    Carr, Natasha B.; Melcher, Cynthia P.

    2015-08-28

    We evaluated Management Questions (Core and Integrated) for each species and community for the Wyoming Basin REA. Core Management Questions address primary management issues, including (1) where is the Conservation Element, and what are its key ecological attributes (characteristics of species and communities that may affect their long-term persistence or viability); (2) what and where are the Change Agents; and (3) how do the Change Agents affect the key ecological attributes? Integrated Management Questions synthesize the Core Management Questions as follows: (1) where are the areas with high landscape-level ecological values; (2) where are the areas with high landscape-level risks; and (3) where are the potential areas for conservation, restoration, and development? The associated maps and key findings for each Management Question are summarized for each Conservation Element in individual chapters. Additional chapters on landscape intactness and an REA synthesis are included.

  20. Metabolic principles of river basin organization.

    PubMed

    Rodriguez-Iturbe, Ignacio; Caylor, Kelly K; Rinaldo, Andrea

    2011-07-19

    The metabolism of a river basin is defined as the set of processes through which the basin maintains its structure and responds to its environment. Green (or biotic) metabolism is measured via transpiration and blue (or abiotic) metabolism through runoff. A principle of equal metabolic rate per unit area throughout the basin structure is developed and tested in a river basin characterized by large heterogeneities in precipitation, vegetation, soil, and geomorphology. This principle is suggested to have profound implications for the spatial organization of river basin hydrologic dynamics, including the minimization of energy expenditure known to control the scale-invariant characteristics of river networks over several orders of magnitude. Empirically derived, remarkably constant rates of average transpiration per unit area through the basin structure lead to a power law for the probability distribution of transpiration from a randomly chosen subbasin. The average runoff per unit area, evaluated for subbasins of a wide range of topological magnitudes, is also shown to be remarkably constant independently of size. A similar result is found for the rainfall after accounting for canopy interception. Allometric scaling of metabolic rates with size, variously addressed in the biological literature and network theory under the label of Kleiber's law, is similarly derived. The empirical evidence suggests that river basin metabolic activity is linked with the spatial organization that takes place around the drainage network and therefore with the mechanisms responsible for the fractal geometry of the network, suggesting a new coevolutionary framework for biological, geomorphological, and hydrologic dynamics.