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Sample records for aldo morselli infn

  1. Enrico Morselli's Psychology and "Spiritism": psychiatry, psychology and psychical research in Italy in the decades around 1900.

    PubMed

    Brancaccio, Maria Teresa

    2014-12-01

    This paper traces Enrico Morselli's intellectual trajectory from the 1870s to the early 1900s. His interest in phenomena of physical mediumship is considered against the backdrop of the theoretical developments in Italian psychiatry and psychology. A leading positivist psychiatrist and a prolific academic, Morselli was actively involved in the making of Italian experimental psychology. Initially sceptical of psychical research and opposed to its association with the 'new psychology', Morselli subsequently conducted a study of the physical phenomena produced by the medium Eusapia Palladino. He concluded that her phenomena were genuine and represented them as the effects of an unknown bio-psychic force present in all human beings. By contextualizing Morselli's study of physical mediumship within contemporary theoretical and disciplinary discourse, this study elaborates shifts in the interpretations of 'supernormal' phenomena put forward by leading Italian psychiatrists and physiologists. It demonstrates that Morselli's interest in psychical research stems from his efforts to comprehend the determinants of complex psychological phenomena at a time when the dynamic theory of matter in physics, and the emergence of neo-vitalist theories influenced the theoretical debates in psychiatry, psychology and physiology. PMID:25218119

  2. Aldo Leopold: An American Prophet

    ERIC Educational Resources Information Center

    Frese, Stephen J.

    2003-01-01

    In 1935, Aldo Leopold bought an abandoned farm in the sand counties along the Wisconsin River near Baraboo. Leopold sensed promise in the land, and with his wife and five children nursed the land back to health. They cleaned out the chicken coop and affectionately called their new family retreat "The Shack." Leopold kept detailed notes during the…

  3. Experimental Nuclear Physics with INFN

    SciTech Connect

    Bracco, Angela

    2009-05-04

    An overview of the experimental activities in Nuclear Physics carried out by Italian researchers and funded by INFN is presented. The experimental program addresses a number of key problems of modern nuclear physics in the fields of hadron dynamics, quark gluon plasma, nuclear structure and reaction dynamics and nuclear astrophysics. The experiments are performed at the four national laboratories, at CERN and several other laboratories abroad. In particular, LNL is mainly dedicated to nuclear structure, LNS is strongly involved in the study of equation of state and nuclear astrophysics, LNF has a program on hypernuclei and kaonic atoms and LNGS has a facility for measurements of cross sections of astrophysical interest. The large community working on the problem of quark gluon plasma is very active in the ALICE experiment which will use the ultrarelativisc heavy ion collisions of LHC. Interdisciplinary researches are also supported. A brief outline of the future perspectives is here given.

  4. Status of the INFN-RDH project

    NASA Astrophysics Data System (ADS)

    Battistoni, Giuseppe

    2015-10-01

    The RDH project is presently coordinating a number of activities for the development of innovative solution for charged particle therapy, on the basis of the scientific and technological know-how in nuclear and particle physics existing within the INFN institute. The status of the project is presented, summarizing the main activities with particular attention to those connected with detector development.

  5. INFN-CNAF Monitor and Control System

    NASA Astrophysics Data System (ADS)

    Antonelli, Stefano; De Girolamo, Donato; dell'Agnello, Luca; Gregori, Daniele; Guizzunti, Guido; Ricci, Pier Paolo; Rosso, Felice; Sapunenko, Vladimir; Veraldi, Riccardo; Veronesi, Paolo; Vistoli, Cristina; Vita Finzi, Giulia; Zani, Stefano

    2011-12-01

    CNAF is the national center of National Institute of Nuclear Physics (INFN) for R&D in the field of Information Technologies applied to High Energy Physics (HEP) experiments. It is involved in the management and development of the most important information and data handling services in behalf of the INFN. In 2005, the Italian Tier-1 for Large Hadron Collider (LHC) experiments has been inaugurated at INFN-CNAF. Due to the huge complexity of Tier-1 center, the use of control systems is fundamental for management and operation of the center. At INFN-CNAF, several solutions have been adopted, from commercial to open source products up to entirely home-made systems. Adopted open source solutions have been strongly adapted to specific needs; a wide set of customized sensors has been developed for various divisions like Network, Storage, Farming, Grid operation and National Services. Finally, a dashboard has been developed, to which described control systems send critical alarms (sent via sms to an operator as well). The dashboard can be exploited to get an historical view of the Tier-1 and national services' state and to allow a quick web control. In this article, the whole system, adopted customizations in monitoring and control as well as their integrations with the dashboard will be described.

  6. 75 FR 33573 - Information Collection; Aldo Leopold Wilderness Research Institute Wilderness Visitor Study

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-14

    ... Forest Service Information Collection; Aldo Leopold Wilderness Research Institute Wilderness Visitor... organizations on the new information collection: Aldo Leopold Wilderness Research Institute Wilderness Visitor...: Comments concerning this notice should be addressed to Alan Watson, Aldo Leopold Wilderness...

  7. Functional studies of aldo-keto reductases in Saccharomyces cerevisiae*

    PubMed Central

    Chang, Qing; Griest, Terry A.; Harter, Theresa M.; Petrash, J. Mark

    2007-01-01

    SUMMARY We utilized the budding yeast Saccharomyces cerevisiae as a model to systematically explore physiological roles for yeast and mammalian aldo-keto reductases. Six open reading frames encoding putative aldo-keto reductases were identified when the yeast genome was queried against the sequence for human aldose reductase, the prototypical mammalian aldo-keto reductase. Recombinant proteins produced from five of these yeast open reading frames demonstrated NADPH-dependent reductase activity with a variety of aldehyde and ketone substrates. A triple aldo-keto reductase null mutant strain demonstrated a glucose-dependent heat shock phenotype which could be rescued by ectopic expression of human aldose reductase. Catalytically-inactive mutants of human or yeast aldo-keto reductases failed to effect a rescue of the heat shock phenotype, suggesting that the phenotype results from either an accumulation of one or more unmetabolized aldo-keto reductase substrates or a synthetic deficiency of aldo-keto reductase products generated in response to heat shock stress. These results suggest that multiple aldo-keto reductases fulfill functionally redundant roles in the stress response in yeast. PMID:17140678

  8. Comparative anatomy of the aldo-keto reductase superfamily.

    PubMed Central

    Jez, J M; Bennett, M J; Schlegel, B P; Lewis, M; Penning, T M

    1997-01-01

    The aldo-keto reductases metabolize a wide range of substrates and are potential drug targets. This protein superfamily includes aldose reductases, aldehyde reductases, hydroxysteroid dehydrogenases and dihydrodiol dehydrogenases. By combining multiple sequence alignments with known three-dimensional structures and the results of site-directed mutagenesis studies, we have developed a structure/function analysis of this superfamily. Our studies suggest that the (alpha/beta)8-barrel fold provides a common scaffold for an NAD(P)(H)-dependent catalytic activity, with substrate specificity determined by variation of loops on the C-terminal side of the barrel. All the aldo-keto reductases are dependent on nicotinamide cofactors for catalysis and retain a similar cofactor binding site, even among proteins with less than 30% amino acid sequence identity. Likewise, the aldo-keto reductase active site is highly conserved. However, our alignments indicate that variation ofa single residue in the active site may alter the reaction mechanism from carbonyl oxidoreduction to carbon-carbon double-bond reduction, as in the 3-oxo-5beta-steroid 4-dehydrogenases (Delta4-3-ketosteroid 5beta-reductases) of the superfamily. Comparison of the proposed substrate binding pocket suggests residues 54 and 118, near the active site, as possible discriminators between sugar and steroid substrates. In addition, sequence alignment and subsequent homology modelling of mouse liver 17beta-hydroxysteroid dehydrogenase and rat ovary 20alpha-hydroxysteroid dehydrogenase indicate that three loops on the C-terminal side of the barrel play potential roles in determining the positional and stereo-specificity of the hydroxysteroid dehydrogenases. Finally, we propose that the aldo-keto reductase superfamily may represent an example of divergent evolution from an ancestral multifunctional oxidoreductase and an example of convergent evolution to the same active-site constellation as the short

  9. Origin and status of the Gran Sasso INFN Laboratory

    NASA Astrophysics Data System (ADS)

    Votano, Lucia

    2014-11-01

    The Gran Sasso National Laboratory of INFN (LNGS) is the largest underground laboratory for astroparticle physics in the world. Located in Italy between the cities of L'Aquila and Teramo, 120 km far from Rome, is a research infrastructure mainly dedicated to astroparticle and neutrino physics. It offers the most advanced underground facility in terms of dimensions, complexity and completeness of its infrastructures. LNGS is one of the four national laboratories run by the Istituto Nazionale di Fisica Nucleare (INFN). The scientific program at LNGS is mainly focused on astroparticle, particle and nuclear physics. The laboratory presently hosts many experiments as well as R&D activities, including world-leading research in the fields of solar neutrinos, accelerator neutrinos (CNGS neutrino beam from CERN to Gran Sasso), dark matter (DM), neutrinoless double beta decay (2β0ν) and nuclear cross-section of astrophysical interest. Associate sciences like earth physics, biology and fundamental physics complement the activities. The laboratory is operated as an international science facility and hosts experiments whose scientific merit is assessed by an international advisory Scientific Committee. A review of the main experiments carried out at LNGS will be given, together with the most recent and relevant scientific results achieved.

  10. Origin and Status of the Gran Sasso INFN Laboratory

    NASA Astrophysics Data System (ADS)

    Votano, Lucia

    2014-06-01

    The Gran Sasso National Laboratory of INFN (LNGS) is the largest underground laboratory for astroparticle physics in the world. Located in Italy between the cities of L'Aquila and Teramo, 120 Km far from Rome, is a research infrastructure mainly dedicated to astroparticle and neutrino physics. It offers the most advanced underground facility in terms of dimensions, complexity and completeness of its infrastructures. LNGS is one of the four national laboratories run by the INFN (Istituto Nazionale di Fisica Nucleare). The scientific program at LNGS is mainly focused on astroparticle, particle and nuclear physics. The laboratory presently hosts many experiments as well as R&D activities, including world-leading research in the fields of solar neutrinos, accelerator neutrinos (CNGS neutrino beam from CERN to Gran Sasso), dark matter, neutrinoless double beta decay and nuclear cross section of astrophysical interest. Associate sciences like earth physics, biology and fundamental physics complement the activities. The laboratory is operated as an international science facility and hosts experiments whose scientific merit is assessed by an international advisory Scientific Committee. A review of the main experiments carried out at LNGS will be given, together with the most recent and relevant scientific results achieved.

  11. DAFNE-Light INFN-LNF Synchrotron Radiation Facility

    SciTech Connect

    Balerna, A.; Cestelli-Guidi, M.; Cimino, R.; Commisso, M.; Grilli, A.; Pietropaoli, M.; Raco, A.; Sciarra, V.; Tullio, V.; Viviani, G.; De Sio, A.; Gambicorti, L.; Hampai, D.; Pace, E.

    2010-06-23

    DAFNE-Light is the Synchrotron Radiation Facility at the INFN-Frascati National Laboratory (Rome, Italy). Three beamlines are operational, using in parasitic and dedicated mode the intense photon emission of DAFNE, a 0.51 GeV storage ring with a routinely circulating electron current higher than 1 Ampere. Two of these beamlines--the soft x-ray (DXR1) and UV (DXR2)--use one of the DAFNE wiggler magnets as synchrotron radiation source, while the third beamline SINBAD (Synchrotron Infrared Beamline At DAFNE) collects the radiation from a bending magnet. New XUV bending magnet beamlines are nowadays under construction and the low energy one (35-200 eV) will be ready for commissioning by the end of 2009. A presentation of the facility will be given together with some recent scientific results achieved at SINBAD and DXR1 beamlines.

  12. The INFN-FBK "Phase-2" R&D program

    NASA Astrophysics Data System (ADS)

    Dalla Betta, G.-F.; Boscardin, M.; Bomben, M.; Brianzi, M.; Calderini, G.; Darbo, G.; Dell'Orso, R.; Gaudiello, A.; Giacomini, G.; Mendicino, R.; Meschini, M.; Messineo, A.; Ronchin, S.; Sultan, D. M. S.; Zorzi, N.

    2016-07-01

    We report on the 3-year INFN ATLAS-CMS joint research activity in collaboration with FBK, started in 2014, and aimed at the development of new thin pixel detectors for the High Luminosity LHC Phase-2 upgrades. The program is concerned with both 3D and planar active-edge pixel sensors to be made on 6" p-type wafers. The technology and the design will be optimized and qualified for extreme radiation hardness (2×1016 neq cm-2). Pixel layouts compatible with present (for testing) and future (RD53 65 nm) front-end chips of ATLAS and CMS are considered. The paper covers the main aspects of the research program, from the sensor design and fabrication technology, to the results of initial tests performed on the first prototypes.

  13. Docker experience at INFN-Pisa Grid Data Center

    NASA Astrophysics Data System (ADS)

    Mazzoni, E.; Arezzini, S.; Boccali, T.; Ciampa, A.; Coscetti, S.; Bonacorsi, D.

    2015-12-01

    Clouds and virtualization offer typical answers to the needs of large-scale computing centers to satisfy diverse sets of user communities in terms of architecture, OS, etc. On the other hand, solutions like Docker seems to emerge as a way to rely on Linux kernel capabilities to package only the applications and the development environment needed by the users, thus solving several resource management issues related to cloud-like solutions. In this paper, we present an exploratory (though well advanced) test done at a major Italian Tier2, at INFN-Pisa, where a considerable fraction of the resources and services has been moved to Docker. The results obtained are definitely encouraging, and Pisa is transitioning all of its Worker Nodes and services to Docker containers. Work is currently being expanded into the preparation of suitable images for a completely virtualized Tier2, with no dependency on local configurations.

  14. Application of the Ta liner technique to produce Ca beams at INFN-Legnaro National Laboratories (INFN-LNL)

    NASA Astrophysics Data System (ADS)

    Galatà, A.; Sattin, M.; Manzolaro, M.; Martini, D.; Facco, A.; Tinschert, K.; Spaedtke, P.; Lang, R.; Kulevoy, T.

    2014-02-01

    The ECR ion sources are able to produce a wide variety of highly charged metallic ion beams thanks to the development of different techniques (ovens, sputtering, direct insertion, metal ions from volatile compounds (MIVOC)). In the case of the ovens, the sticking of the hot vapors on the surface of the plasma chamber leads to high material consumption rates. For elements like Ca, a tantalum liner inserted inside the chamber can be used to limit this phenomenon. The modeling of temperature distribution inside the chamber with and without the liner was carried out with COMSOL-multiphysics code. Results of simulation and the comparison with experiments performed at INFN-Legnaro National Laboratories with Ca beams are discussed.

  15. Application of the Ta liner technique to produce Ca beams at INFN-Legnaro National Laboratories (INFN-LNL)

    SciTech Connect

    Galatà, A. Sattin, M.; Manzolaro, M.; Martini, D.; Facco, A.; Tinschert, K.; Spaedtke, P.; Lang, R.; Kulevoy, T.

    2014-02-15

    The ECR ion sources are able to produce a wide variety of highly charged metallic ion beams thanks to the development of different techniques (ovens, sputtering, direct insertion, metal ions from volatile compounds (MIVOC)). In the case of the ovens, the sticking of the hot vapors on the surface of the plasma chamber leads to high material consumption rates. For elements like Ca, a tantalum liner inserted inside the chamber can be used to limit this phenomenon. The modeling of temperature distribution inside the chamber with and without the liner was carried out with COMSOL-multiphysics code. Results of simulation and the comparison with experiments performed at INFN-Legnaro National Laboratories with Ca beams are discussed.

  16. To Learn Is To Grow, I: Aldo Leopold, Predator Eradication, and Games Refuges.

    ERIC Educational Resources Information Center

    Dolph, Gary E.

    1998-01-01

    Follows the evolution in the thinking of Aldo Leopold, a game manager who was initially an advocate of predator eradication but who came to see predators as playing an important role in normally functioning ecosystems. (DDR)

  17. Recent progress in plasma modelling at INFN-LNS

    NASA Astrophysics Data System (ADS)

    Neri, L.; Castro, G.; Torrisi, G.; Galatà, A.; Mascali, D.; Celona, L.; Gammino, S.

    2016-02-01

    At Istituto Nazionale di Fisica Nucleare - Laboratori Nazionali del Sud (INFN-LNS), the development of intense ion and proton sources has been supported by a great deal of work on the modelling of microwave generated plasmas for many years. First, a stationary version of the particle-in-cell code was developed for plasma modelling starting from an iterative strategy adopted for the space charge dominated beam transport simulations. Electromagnetic properties of the plasma and full-waves simulations are now affordable for non-homogenous and non-isotropic magnetized plasma via "cold" approximation. The effects of Coulomb collisions on plasma particles dynamics was implemented with the Langevin formalism, instead of simply applying the Spitzer 90° collisions through a Monte Carlo technique. A wide database of different cross sections related to reactions occurring in a hydrogen plasma was implemented. The next step consists of merging such a variety of approaches for retrieving an "as-a-whole" picture of plasma dynamics in ion sources. The preliminary results will be summarized in the paper for a microwave discharge ion source designed for intense and high quality proton beams production, proton source for European Spallation Source project. Even if the realization of a predictive software including the complete processes involved in plasma formation is still rather far, a better comprehension of the source behavior is possible and so the simulations may support the optimization phase.

  18. Recent progress in plasma modelling at INFN-LNS.

    PubMed

    Neri, L; Castro, G; Torrisi, G; Galatà, A; Mascali, D; Celona, L; Gammino, S

    2016-02-01

    At Istituto Nazionale di Fisica Nucleare - Laboratori Nazionali del Sud (INFN-LNS), the development of intense ion and proton sources has been supported by a great deal of work on the modelling of microwave generated plasmas for many years. First, a stationary version of the particle-in-cell code was developed for plasma modelling starting from an iterative strategy adopted for the space charge dominated beam transport simulations. Electromagnetic properties of the plasma and full-waves simulations are now affordable for non-homogenous and non-isotropic magnetized plasma via "cold" approximation. The effects of Coulomb collisions on plasma particles dynamics was implemented with the Langevin formalism, instead of simply applying the Spitzer 90° collisions through a Monte Carlo technique. A wide database of different cross sections related to reactions occurring in a hydrogen plasma was implemented. The next step consists of merging such a variety of approaches for retrieving an "as-a-whole" picture of plasma dynamics in ion sources. The preliminary results will be summarized in the paper for a microwave discharge ion source designed for intense and high quality proton beams production, proton source for European Spallation Source project. Even if the realization of a predictive software including the complete processes involved in plasma formation is still rather far, a better comprehension of the source behavior is possible and so the simulations may support the optimization phase. PMID:26931913

  19. Francium trapping at the INFN-LNL facility

    NASA Astrophysics Data System (ADS)

    Mariotti, E.; Khanbekyan, A.; Marinelli, C.; Marmugi, L.; Moi, L.; Corradi, L.; Dainelli, A.; Calabrese, R.; Mazzocca, G.; Tomassetti, L.

    2014-05-01

    A brief review of the Francium trapping experiments at the INFN-LNL facility is presented in the wide context of Atomic Parity-Nonconservation (APNC), which, as long as acquiring more precise and new spectroscopic data on the Francium isotopes, is the ultimate goal of the experiment. Due to its instability, Francium atoms must be produced continuously by a nuclear fusion-evaporation reaction into a heated Gold target hit by a beam of accelerated oxygen ions. Francium is then extracted in the ionic form and guided by an electrostatic line to the actual science chamber, where the ions are neutralized. Atoms are then cooled down and trapped in a Magneto-Optical Trap (MOT) to ensure both the availability of a sufficiently populated and stable atomic sample and to eliminate the Doppler broadening which would affect the precision of the measurements. A review of the recent improvements to the experimental apparatus and to the detection techniques that led to a sensitivity better than five atoms is presented. The final part of this paper deals with a summary of the recent results obtained by our collaboration and a short outlook for the immediate future.

  20. Future developments of INFN-LNL nuclear beam facilities

    NASA Astrophysics Data System (ADS)

    Bisoffi, G.

    2007-11-01

    The accelerator group at INFN-LNL has been mostly engaged, recently, in completing and commissioning the higher current injector of the linac booster ALPI (named PIAVE) and in constructing and assembling the front-end part of a high current driver linac for the RNB facility SPES. PIAVE, designed to accelerate ions with A/Q = < 8.5 up to 1.2 MeV/u, is now completed. The injector has been commissioned with O, Ar, Ne and Xe beams. Neon and argon beams have been delivered to experiments for a total of about 400 hours. A consolidation program of PIAVE and ALPI is planned, so as to deliver a larger variety of beams with a current range 10 div 100 pnA and with an energy exceeding the Coulomb barrier in relevant nuclear reaction cases. The RNB facility SPES, allowing a frontier program in RNB physics, is being designed and prototyped: beams of neutron rich medium-to-heavy mass nuclei will be produced inducing 238U fission with a 40 MeV 200μA proton beam impinging onto a multi-slice direct target. A further development of ALPI will make it best suitable for the re-acceleration of radioactive nuclear species, after charge breeding and isotope selection.

  1. Life as a sober citizen: Aldo Leopold's Wildlife Ecology 118

    NASA Astrophysics Data System (ADS)

    Theiss, Nancy Stearns

    This historic case study addressed the issue of the lack of citizen action toward environmentally responsible behavior. Although there have been studies regarding components of environmental responsible behavior [ERB], there has been little focus on historic models of exemplary figures of ERB. This study examined one of the first conservation courses in the United States, Wildlife Ecology 118, taught by Aldo Leopold (1887--1948) for 13 years at the University of Wisconsin. Today, Aldo Leopold is recognized as an exemplary conservationist whose land ethic is cited as providing the ecological approach needed for understanding the complex issues of modern society. The researcher conjectured that examination of one of the first environmental education courses could support and strengthen environmental education practices by providing a heuristic perspective. The researcher used two different strategies for analysis of the case. For Research Question One---"What were Leopold's teaching strategies in Wildlife Ecology 118?"---the researcher used methods of comparative historical analysis. The researcher examined the learning outcomes that Leopold used in Wildlife Ecology 118 and compared them against a rubric of the Four Strands for Environmental Education (North American Association for Environmental Education [NAAEE], 1999). The Four Strands for Environmental Education are the current teaching strategies used by educators. The results indicated that Wildlife Ecology 118 scored high in Knowledge of Processes and Systems and Environmental Problem Solving strands. Leopold relied on historic case examples and animal biographies to build stories that engaged students. Field trips gave students practical experience for environmental knowledge with special emphasis on phenology. For Research Question Two---"What was the context of the lessons in Wildlife Ecology 118?"---the researcher used environmental history methods for analysis. Context provided the knowledge and

  2. Aldo-Keto Reductases 1B in Adrenal Cortex Physiology

    PubMed Central

    Pastel, Emilie; Pointud, Jean-Christophe; Martinez, Antoine; Lefrançois-Martinez, A. Marie

    2016-01-01

    Aldose reductase (AKR1B) proteins are monomeric enzymes, belonging to the aldo-keto reductase (AKR) superfamily. They perform oxidoreduction of carbonyl groups from a wide variety of substrates, such as aliphatic and aromatic aldehydes or ketones. Due to the involvement of human aldose reductases in pathologies, such as diabetic complications and cancer, AKR1B subgroup enzymatic properties have been extensively characterized. However, the issue of AKR1B function in non-pathologic conditions remains poorly resolved. Adrenal activities generated large amount of harmful aldehydes from lipid peroxidation and steroidogenesis, including 4-hydroxynonenal (4-HNE) and isocaproaldehyde (4-methylpentanal), which can both be reduced by AKR1B proteins. More recently, some AKR1B isoforms have been shown to be endowed with prostaglandin F synthase (PGFS) activity, suggesting that, in addition to possible scavenger function, they could instigate paracrine signals. Interestingly, the adrenal gland is one of the major sites for human and murine AKR1B expression, suggesting that their detoxifying/signaling activity could be specifically required for the correct handling of adrenal function. Moreover, chronic effects of ACTH result in a coordinated regulation of genes encoding the steroidogenic enzymes and some AKR1B isoforms. This review presents the molecular mechanisms accounting for the adrenal-specific expression of some AKR1B genes. Using data from recent mouse genetic models, we will try to connect their enzymatic properties and regulation with adrenal functions. PMID:27499746

  3. Risk management and the wisdom of Aldo Leopold.

    PubMed

    Warren, Julianne Lutz; Kieffer, Susan

    2010-02-01

    Human demands on nature have increased due to our burgeoning population. The applications of scientific knowledge to the development of increasingly powerful technologies and consumptive lifestyles by more and more people have created a modern category of human-caused disaster-stealth disasters. Stealth disasters-such as agriculturally-induced soil erosion and release of unprecedented amounts of greenhouse gases into Earth's atmosphere-tend to have protracted, unobvious onsets; do not necessarily have dramatic manifestations; and often do not attract public attention until they reach a stage approaching catastrophic consequences. At this late stage it is difficult or impossible to undo damage. Scientists tend to be among the first to understand the physical causes and notice the developments of stealth disasters and their risks and yet scientific knowledge is not enough to prevent or mitigate them. As we search for ways to deal with stealth disasters, the concept of "land health" assembled by the prominent conservationist and author, Aldo Leopold (1887-1948), can, in normative terms, provide an ecologically grounded example of nature in good condition toward which society can aim. Evidence of the reverse-symptoms of land illness-can provide a checklist for risk analysis and management that helps guide people away from harm-causing attitudes and activities and toward beneficial outcomes. Leopold's criteria of land health motivated by a land ethic that incorporates the whole of nature may be applied at geographic scales ranging from local to global as a framework for contemporary risk management. PMID:20136743

  4. Aldo-keto reductase (AKR) superfamily: genomics and annotation.

    PubMed

    Mindnich, Rebekka D; Penning, Trevor M

    2009-07-01

    Aldo-keto reductases (AKRs) are phase I metabolising enzymes that catalyse the reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H)-dependent reduction of carbonyl groups to yield primary and secondary alcohols on a wide range of substrates, including aliphatic and aromatic aldehydes and ketones, ketoprostaglandins, ketosteroids and xenobiotics. In so doing they functionalise the carbonyl group for conjugation (phase II enzyme reactions). Although functionally diverse, AKRs form a protein superfamily based on their high sequence identity and common protein fold, the (alpha/beta) 8 -barrel structure. Well over 150 AKR enzymes, from diverse organisms, have been annotated so far and given systematic names according to a nomenclature that is based on multiple protein sequence alignment and degree of identity. Annotation of non-vertebrate AKRs at the National Center for Biotechnology Information or Vertebrate Genome Annotation (vega) database does not often include the systematic nomenclature name, so the most comprehensive overview of all annotated AKRs is found on the AKR website (http://www.med.upenn.edu/akr/). This site also hosts links to more detailed and specialised information (eg on crystal structures, gene expression and single nucleotide polymorphisms [SNPs]). The protein-based AKR nomenclature allows unambiguous identification of a given enzyme but does not reflect the wealth of genomic and transcriptomic variation that exists in the various databases. In this context, identification of putative new AKRs and their distinction from pseudogenes are challenging. This review provides a short summary of the characteristic features of AKR biochemistry and structure that have been reviewed in great detail elsewhere, and focuses mainly on nomenclature and database entries of human AKRs that so far have not been subject to systematic annotation. Recent developments in the annotation of SNP and transcript variance in AKRs are also summarised. PMID:19706366

  5. Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae.

    PubMed Central

    Kuhn, A; van Zyl, C; van Tonder, A; Prior, B A

    1995-01-01

    A cytosolic aldo-keto reductase was purified from Saccharomyces cerevisiae ATCC 26602 to homogeneity by affinity chromatography, chromatofocusing, and hydroxylapatite chromatography. The relative molecular weights of the aldo-keto reductase as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and size exclusion chromatography were 36,800 and 35,000, respectively, indicating that the enzyme is monomeric. Amino acid composition and N-terminal sequence analysis revealed that the enzyme is closely related to the aldose reductases of xylose-fermenting yeasts and mammalian tissues. The enzyme was apparently immunologically unrelated to the aldose reductases of other xylose-fermenting yeasts. The aldo-keto reductase is NADPH specific and catalyzes the reduction of a variety of aldehydes. The best substrate for the enzyme is the aromatic aldehyde p-nitrobenzaldehyde (Km = 46 microM; kcat/Km = 52,100 s-1 M-1), whereas among the aldoses, DL-glyceraldehyde was the preferred substrate (Km = 1.44 mM; kcat/Km = 1,790 s-1 M-1). The enzyme failed to catalyze the reduction of menadione and p-benzoquinone, substrates for carbonyl reductase. The enzyme was inhibited only slightly by 2 mM sodium valproate and was activated by pyridoxal 5'-phosphate. The optimum pH of the enzyme is 5. These data indicate that the S. cerevisiae aldo-keto reductase is a monomeric NADPH-specific reductase with strong similarities to the aldose reductases. PMID:7747971

  6. Framing a Philosophy of Environmental Action: Aldo Leopold, John Muir, and the Importance of Community

    ERIC Educational Resources Information Center

    Goralnik, Lissy; Nelson, Michael P.

    2011-01-01

    A philosophy of action consists of a theory about how and why we do things and what motivates us to act. By juxtaposing the theory of environmental action implied by the works and life of John Muir with the philosophy of action suggested by Aldo Leopold's Land Ethic, we will illuminate the importance of a philosophy of action in determining one's…

  7. INFN-Pisa scientific computation environment (GRID, HPC and Interactive Analysis)

    NASA Astrophysics Data System (ADS)

    Arezzini, S.; Carboni, A.; Caruso, G.; Ciampa, A.; Coscetti, S.; Mazzoni, E.; Piras, S.

    2014-06-01

    The INFN-Pisa Tier2 infrastructure is described, optimized not only for GRID CPU and Storage access, but also for a more interactive use of the resources in order to provide good solutions for the final data analysis step. The Data Center, equipped with about 6700 production cores, permits the use of modern analysis techniques realized via advanced statistical tools (like RooFit and RooStat) implemented in multicore systems. In particular a POSIX file storage access integrated with standard SRM access is provided. Therefore the unified storage infrastructure is described, based on GPFS and Xrootd, used both for SRM data repository and interactive POSIX access. Such a common infrastructure allows a transparent access to the Tier2 data to the users for their interactive analysis. The organization of a specialized many cores CPU facility devoted to interactive analysis is also described along with the login mechanism integrated with the INFN-AAI (National INFN Infrastructure) to extend the site access and use to a geographical distributed community. Such infrastructure is used also for a national computing facility in use to the INFN theoretical community, it enables a synergic use of computing and storage resources. Our Center initially developed for the HEP community is now growing and includes also HPC resources fully integrated. In recent years has been installed and managed a cluster facility (1000 cores, parallel use via InfiniBand connection) and we are now updating this facility that will provide resources for all the intermediate level HPC computing needs of the INFN theoretical national community.

  8. The Long Term Data Preservation (LTDP) project at INFN CNAF: CDF use case

    NASA Astrophysics Data System (ADS)

    Amerio, S.; Chiarelli, L.; Dell'Agnello, L.; Gregori, D.; Pezzi, M.; Ricci, P.; Rosso, F.; Zani, S.

    2015-05-01

    In the last years the problem of preservation of scientific data has become one of the most important topics inside international scientific communities. In particular the long term preservation of experimental data, raw and all related derived formats including calibration information, is one of the emerging requirements within the High Energy Physics (HEP) community for experiments that have already concluded the data taking phase. The DPHEP group (Data Preservation in HEP) coordinates the local teams within the whole collaboration and the different Tiers (computing centers). The INFN-CNAF Tier-1 is one of the reference sites for data storage and computing in the LHC community but it also offers resources to many other HEP and non-HEP collaborations. In particular the CDF experiment has used the INFN-CNAF Tier-1 resources for many years and after the end of data taking in 2011, it is now facing the challenge to both preserve the large amount of data produced during several years and to retain the ability to access and reuse the whole amount of it in the future. According to this task the CDF Italian collaboration, together with the INFN-CNAF computing center, has developed and is now implementing a long term future data preservation project in collaboration with Fermilab (FNAL) computing sector. The project comprises the copy of all CDF raw data and user level ntuples (about 4 PB) at the INFN-CNAF site and the setup of a framework which will allow to access and analyze the data in the long term future. A portion of the 4 PB of data (raw data and analysis-level ntuples) are currently being copied from FNAL to the INFN-CNAF tape library backend and a system to allow data access is being setup. In addition to this data access system, a data analysis framework is being developed in order to run the complete CDF analysis chain in the long term future, from raw data reprocessing to analysis-level ntuples production and analysis. In this contribution we first illustrate

  9. Production of keto-disaccharides from aldo-disaccharides in subcritical aqueous ethanol.

    PubMed

    Gao, Da-Ming; Kobayashi, Takashi; Adachi, Shuji

    2016-05-01

    Isomerization of disaccharides (maltose, isomaltose, cellobiose, lactose, melibiose, palatinose, sucrose, and trehalose) was investigated in subcritical aqueous ethanol. A marked increase in the isomerization of aldo-disaccharides to keto-disaccharides was noted and their hydrolytic reactions were suppressed with increasing ethanol concentration. Under any study condition, the maximum yield of keto-disaccharides produced from aldo-disaccharides linked by β-glycosidic bond was higher than that produced from aldo-disaccharides linked by α-glycosidic bond. Palatinose, a keto-disaccharide, mainly underwent decomposition rather than isomerization in subcritical water and subcritical aqueous ethanol. No isomerization was noted for the non-reducing disaccharides trehalose and sucrose. The rate constant of maltose to maltulose isomerization almost doubled by changing solvent from subcritical water to 80 wt% aqueous ethanol at 220 °C. Increased maltose monohydrate concentration in feed decreased the conversion of maltose and the maximum yield of maltulose, but increased the productivity of maltulose. The maximum productivity of maltulose was ca. 41 g/(h kg-solution). PMID:26786171

  10. The Aldo-Keto Reductase Superfamily and its Role in Drug Metabolism and Detoxification

    PubMed Central

    Barski, Oleg A.; Tipparaju, Srinivas M.; Bhatnagar, Aruni

    2008-01-01

    The Aldo-Keto Reductase (AKR) superfamily comprises of several enzymes that catalyze redox transformations involved in biosynthesis, intermediary metabolism and detoxification. Substrates of the family include glucose, steroids, glycosylation end products, lipid peroxidation products, and environmental pollutants. These proteins adopt a (β/α)8 barrel structural motif interrupted by a number of extraneous loops and helixes that vary between proteins and bring structural identity to individual families. The human AKR family differs from the rodent families. Due to their broad substrate specificity, AKRs play an important role in the Phase II detoxification of a large number of pharmaceuticals, drugs, and xenobiotics. PMID:18949601

  11. Aldo-keto reductase family 1 member B8 is secreted via non-classical pathway

    PubMed Central

    Tang, Zhenwang; Xia, Chenglai; Huang, Renbin; Li, Xiaoning; Wang, Wan-Chun; Guo, Wangyuan; Duan, Lili; Luo, Weihao; Cao, Deliang; Luo, Di-Xian

    2014-01-01

    Mouse aldo-keto reductase family 1 member B8 (AKR1B8) has the highest similarity to human aldo-keto reductase family 1 member B10 (AKR1B10), a secretory protein through lysosomes-mediated non-classical secretory pathway. To identify whether AKR1B8 is secreted through the same pathway, we carried out this study. Self-developed sandwich ELISA and western blot were used to detect AKR1B8 in cells and culture medium of CT-26 murine colon carcinoma cells. AKR1B8 releases in an independent manner to Brefeldin A, an inhibitor of ER-to-Golgi classical secretion pathway. Several factors, which are involved in the non-classical secretion pathway, such as temperature, ATP and calcium ion, regulated AKR1B8 secretion from mouse colorectal cancer cells CT-26. Lysosomotropic NH4Cl increased AKR1B8 secretion, and AKR1B8 was located in isolated lysosomes. Therefore, AKR1B8 is a new secretory protein through the lysosomes-mediated non-classical pathway. PMID:25120755

  12. The “NetBoard”: Network Monitoring Tools Integration for INFN Tier-1 Data Center

    NASA Astrophysics Data System (ADS)

    De Girolamo, D.; dell'Agnello and, L.; Zani, S.

    2012-12-01

    The monitoring and alert system is fundamental for the management and the operation of the network in a large data center such as an LHC Tier-1. The network of the INFN Tier-1 at CNAF is a multi-vendor environment: for its management and monitoring several tools have been adopted and different sensors have been developed. In this paper, after an overview on the different aspects to be monitored and the tools used for them (i.e. MRTG, Nagios, Arpwatch, NetFlow, Syslog, etc), we will describe the “NetBoard”, a monitoring toolkit developed at the INFN Tier-1. NetBoard, developed for a multi-vendor network, is able to install and auto-configure all tools needed for its monitoring, either via network devices discovery mechanism or via configuration file or via wizard. In this way, we are also able to activate different types of sensors and Nagios checks according to the equipment vendor specifications. Moreover, when a new device is connected in the LAN, NetBoard can detect where it is plugged. Finally the NetBoard web interface allows to have the overall status of the entire network “at a glance”, both the local and the geographical (including the LHCOPN and the LHCONE) link utilization, health status of network devices (with active alerts) and flow analysis.

  13. Aldo Leopold on Education: An Educator and His Land Ethic in the Context of Contemporary Environmental Education.

    ERIC Educational Resources Information Center

    Callicott, J. Baird

    1982-01-01

    Aldo Leopold, the founder of wildlife management (wildlife ecology) is credited with powerfully advocating for the first time in Western intellectual history, broad human ethical responsibility to the nonhuman natural world. Leopold's views on education and Leopold as an educator are discussed. (Author/JN)

  14. Cryogenic Control System Migration and Developments towards the UNICOS CERN Standard at INFN

    NASA Astrophysics Data System (ADS)

    Modanese, Paolo; Calore, Andrea; Contran, Tiziano; Friso, Alessandro; Pengo, Marco; Canella, Stefania; Burioli, Sergio; Gallese, Benedetto; Inglese, Vitaliano; Pezzetti, Marco; Pengo, Ruggero

    The cryogenic control systems at Laboratori Nazionali di Legnaro (LNL) are undergoing an important and radical modernization, allowing all the plants controls and supervision systems to be renewed in a homogeneous way towards the CERN-UNICOS standard. Before the UNICOS migration project started there were as many as 7 different types of PLC and 7 different types of SCADA, each one requiring its own particular programming language. In these conditions, even a simple modification and/or integration on the program or on the supervision, required the intervention of a system integrator company, specialized in its specific control system. Furthermore it implied that the operators have to be trained to learn the different types of control systems. The CERN-UNICOS invented for LHC [1] has been chosen due to its reliability and planned to run and be maintained for decades on. The complete migration is part of an agreement between CERN and INFN.

  15. The INFN-CNAF Tier-1 GEMSS Mass Storage System and database facility activity

    NASA Astrophysics Data System (ADS)

    Ricci, Pier Paolo; Cavalli, Alessandro; Dell'Agnello, Luca; Favaro, Matteo; Gregori, Daniele; Prosperini, Andrea; Pezzi, Michele; Sapunenko, Vladimir; Zizzi, Giovanni; Vagnoni, Vincenzo

    2015-05-01

    The consolidation of Mass Storage services at the INFN-CNAF Tier1 Storage department that has occurred during the last 5 years, resulted in a reliable, high performance and moderately easy-to-manage facility that provides data access, archive, backup and database services to several different use cases. At present, the GEMSS Mass Storage System, developed and installed at CNAF and based upon an integration between the IBM GPFS parallel filesystem and the Tivoli Storage Manager (TSM) tape management software, is one of the largest hierarchical storage sites in Europe. It provides storage resources for about 12% of LHC data, as well as for data of other non-LHC experiments. Files are accessed using standard SRM Grid services provided by the Storage Resource Manager (StoRM), also developed at CNAF. Data access is also provided by XRootD and HTTP/WebDaV endpoints. Besides these services, an Oracle database facility is in production characterized by an effective level of parallelism, redundancy and availability. This facility is running databases for storing and accessing relational data objects and for providing database services to the currently active use cases. It takes advantage of several Oracle technologies, like Real Application Cluster (RAC), Automatic Storage Manager (ASM) and Enterprise Manager centralized management tools, together with other technologies for performance optimization, ease of management and downtime reduction. The aim of the present paper is to illustrate the state-of-the-art of the INFN-CNAF Tier1 Storage department infrastructures and software services, and to give a brief outlook to forthcoming projects. A description of the administrative, monitoring and problem-tracking tools that play a primary role in managing the whole storage framework is also given.

  16. Berberine inhibits androgen synthesis by interaction with aldo-keto reductase 1C3 in 22Rv1 prostate cancer cells

    PubMed Central

    Tian, Yuantong; Zhao, Lijing; Wang, Ye; Zhang, Haitao; Xu, Duo; Zhao, Xuejian; Li, Yi; Li, Jing

    2016-01-01

    Aldo-keto reductase family 1 member C3 has recently been regarded as a potential therapeutic target in castrate-resistant prostate cancer. Herein, we investigated whether berberine delayed the progression of castrate-resistant prostate cancer by reducing androgen synthesis through the inhibition of Aldo-keto reductase family 1 member C3. Cell viability and cellular testosterone content were measured in prostate cancer cells. Aldo-keto reductase family 1 member C3 mRNA and protein level were detected by RT-PCR and Western bolt analyses, respectively. Computer analysis with AutoDock Tools explored the molecular interaction of berberine with Aldo-keto reductase family 1 member C3. We found that berberine inhibited 22Rv1 cells proliferation and decreased cellular testosterone formation in a dose-dependent manner. Berberine inhibited Aldo-keto reductase family 1 member C3 enzyme activity, rather than influenced mRNA and protein expressions. Molecular docking study demonstrated that berberine could enter the active center of Aldo-keto reductase family 1 member C3 and form π-π interaction with the amino-acid residue Phe306 and Phe311. In conclusion, the structural interaction of berberine with Aldo-keto reductase family 1 member C3 is attributed to the suppression of Aldo-keto reductase family 1 member C3 enzyme activity and the inhibition of 22Rv1 prostate cancer cell growth by decreasing the intracellular androgen synthesis. Our result provides the experimental basis for the design, research, and development of AKR1C3 inhibitors using berberine as the lead compound. PMID:26698234

  17. Berberine inhibits androgen synthesis by interaction with aldo-keto reductase 1C3 in 22Rv1 prostate cancer cells.

    PubMed

    Tian, Yuantong; Zhao, Lijing; Wang, Ye; Zhang, Haitao; Xu, Duo; Zhao, Xuejian; Li, Yi; Li, Jing

    2016-01-01

    Aldo-keto reductase family 1 member C3 has recently been regarded as a potential therapeutic target in castrate-resistant prostate cancer. Herein, we investigated whether berberine delayed the progression of castrate-resistant prostate cancer by reducing androgen synthesis through the inhibition of Aldo-keto reductase family 1 member C3. Cell viability and cellular testosterone content were measured in prostate cancer cells. Aldo-keto reductase family 1 member C3 mRNA and protein level were detected by RT-PCR and Western bolt analyses, respectively. Computer analysis with AutoDock Tools explored the molecular interaction of berberine with Aldo-keto reductase family 1 member C3. We found that berberine inhibited 22Rv1 cells proliferation and decreased cellular testosterone formation in a dose-dependent manner. Berberine inhibited Aldo-keto reductase family 1 member C3 enzyme activity, rather than influenced mRNA and protein expressions. Molecular docking study demonstrated that berberine could enter the active center of Aldo-keto reductase family 1 member C3 and form p-p interaction with the amino-acid residue Phe306 and Phe311. In conclusion, the structural interaction of berberine with Aldo-keto reductase family 1 member C3 is attributed to the suppression of Aldo-keto reductase family 1 member C3 enzyme activity and the inhibition of 22Rv1 prostate cancer cell growth by decreasing the intracellular androgen synthesis. Our result provides the experimental basis for the design, research, and development of AKR1C3 inhibitors using berberine as the lead compound. PMID:26698234

  18. X-band accelerator structures: On going R&D at the INFN

    NASA Astrophysics Data System (ADS)

    Gatti, G.; Marcelli, A.; Spataro, B.; Dolgashev, V.; Lewandowski, J.; Tantawi, S. G.; Yeremian, A. D.; Higashi, Y.; Rosenzweig, J.; Sarti, S.; Caliendo, C.; Castorina, G.; Cibin, G.; Carfora, L.; Leonardi, O.; Rigato, V.; Campostrini, M.

    2016-09-01

    The next generation of accelerators, from the compact to the large infrastructure dedicated to high energy physics, is highly demanding in terms of accelerating gradients. To upgrade performances of X band linacs at 11.424 GHz many resources are devoted to achieve high accelerating gradients and at the same time to obtain a high reliability. In the framework of a three-year funded project by the Vth Committee of the INFN to the Laboratori Nazionali di Frascati (LNF) and to the Laboratori Nazionali di Legnaro (LNL). Within a broad international collaboration the LNF has been involved in the design, manufacture and test of compact high power standing wave (SW) sections operating at high frequency while LNL is actively involved in the development of new materials and multilayers using PVD (Physical Vapor Deposition) methods. We will report about the status of the accelerating device and of the different ongoing R&D activities and characterization procedures such as tests of different materials and metallic coatings.

  19. INFN - P.L.A.I.A. PROJECT (Plasma Laser Ablation for Ion Acceleration)

    NASA Astrophysics Data System (ADS)

    Torrisi, L.; Gammino, S.; Andò, L.; Ciavola, G.; Mezzasalma, A. M.; Nassisi, V.; Wolowski, J.; Parys, P.; Laska, L.; Krasa, J.; Boody, F. P.

    2004-10-01

    The INFN-Gr.V PLAIA (Plasma Laser Ablation for Ion Acceleration) Project is presented and discussed. The project is developing at LNS of Catania, Messina and Lecce Laboratories as Italian centers of research and it see as European partners the PALS Laboratory of Prague and the group of researchers coordinated by Prof. Wolowsky from IPPLM of Warsaw. PLAIA concerns the study of pulsed plasma produced by pulsed lasers and some special applications of this physics to the new generation of ion sources. Different lasers are employed at LNS of Catania, LEA of Lecce and PALS of Prague. Their fluences range from about 10 J/cm2 for the excimer lasers of LEA up to about 100 kj/cm2 for the iodine laser of PALS. The Nd:Yag laser of LNS, operating at 1064 nm, 9 ns pulse width and 900 mJ maximum pulse energy shows peculiar properties, specially if it is employed at 30 Hz repetition rate, at which it may produce stabile current of ions ejected from a dense plasma. Such laser has the optimum compromise between power density and repetition rate to be used as injector of ions in ECR sources or as source of a new generation of ion implanters which can be employed to accelerate multi-energetic ion beams useful to treat the surface of different materials. Results and projects are discussed in detail.

  20. Progress in the design and construction of SPES at INFN-LNL

    NASA Astrophysics Data System (ADS)

    Bisoffi, G.; Prete, G.; Andrighetto, A.; Andreev, V.; Bellan, L.; Bellato, M.; Bortolato, D.; Calderolla, M.; Canella, S.; Comunian, M.; Corradetti, S.; Fagotti, E.; Facco, A.; Favaron, P.; Ferrari, L.; Galatà, A.; Giacchini, M.; Gramegna, F.; Gulmini, M.; Lombardi, A.; Maggiore, M.; Manzolaro, M.; Modanese, P.; Moisio, M. F.; Monetti, A.; Palmieri, A.; Pisent, A.; Poggi, M.; Porcellato, A. M.; Rossignoli, M.; Roncolato, C.; Russo, A.; Sarchiapone, L.; Scarpa, D.; Vasquez, J.; Zafiropoulos, D.

    2016-06-01

    INFN-LNL is constructing an ISOL (Isotope Separation On Line) facility delivering neutron rich ion beams at 10 A MeV or beyond, making use of the linear accelerator ALPI as the secondary accelerator. The facility includes a direct ISOL target based on UCx and able to reach 1013 fissions/s. In parallel, an applied physics facility will be developed, with applications in medicine and neutron production. The SPES project is a national facility, approved and funded. Commissioning with the first exotic species is expected in 2019. The primary accelerator is a commercial cyclotron, which will send a 40 MeV, 200 μA proton beam onto an UCx target, connected to SIS, PIS and LIS ion sources. The extracted beam is purified through a Low Resolution Mass Separator (LMRS, i.e. a Wien filter and a dispersive dipole), a beam cooler and a High Resolution Mass Separator (HRMS) and sent to an ECR charge breeder to boost the exotic beam charge state. The highly charged exotic beam is further separated in a MRMS (Medium Resolution Mass Separator) and injected into a 100% duty cycle RFQ and into the existing superconducting linac ALPI, which will be refurbished and upgraded to be an efficient exotic beam accelerator. The upgrade of ALPI will give ∼10 A MeV energy to 132Sn19+, taken as the reference ion beam. The paper presents the status of the design and construction of the SPES facility.

  1. MEG II drift chamber characterization with the silicon based cosmic ray tracker at INFN Pisa

    NASA Astrophysics Data System (ADS)

    Venturini, M.; Baldini, A. M.; Baracchini, E.; Cei, F.; D`Onofrio, A.; Dussoni, S.; Galli, L.; Grassi, M.; Nicolò, D.; Signorelli, G.

    2016-07-01

    High energy physics experiments at the high intensity frontier place ever greater demands on detectors, and in particular on tracking devices. In order to compare the performance of small size tracking prototypes, a high resolution cosmic ray tracker has been assembled to provide an external track reference. It consists of four spare ladders of the external layers of the Silicon Vertex Tracker of the BaBar experiment. The test facility, operating at INFN Sezione di Pisa, provides the detector under test with an external track with an intrinsic resolution of 15-30 μm. The MEG II tracker is conceived as a unique volume wire drift chamber filled with He-isobutane 85-15%. The ionization density in this gas mixture is about 13 clusters/cm and this results in a non-negligible bias of the impact parameters for tracks crossing the cell close to the anode wire. We present the telescope performance in terms of tracking efficiency and resolution and the results of the characterization of a MEG II drift chamber prototype.

  2. The Injection System of the INFN-SuperB Factory Project: Preliminary Design

    SciTech Connect

    Boni, Roberto; Guiducci, Susanna; Preger, Miro; Raimondi, Pantaleo; Chance, Antoine; Dadoun, Olivier; Poirier, Freddy; Variola, Alessandro; Seeman, John; /SLAC

    2012-07-05

    The ultra high luminosity B-factory (SuperB) project of INFN requires a high performance and reliable injection system, providing electrons at 4 GeV and positrons at 7 GeV, to fulfil the very tight requirements of the collider. Due to the short beam lifetime, continuous injection of electron and positron bunches in both LER and HER rings is necessary to maintain an high average luminosity. Polarized electrons are required for experiments and must be delivered by the injection system, due to the beam lifetime shorter than the ring polarization build-up: they will be produced by means of a SLAC-SLC polarized gun. The emittance and the energy spread of the e{sup -}/e{sup +} beams are reduced in a 1 GeV Damping Ring (DR) before injection in the main rings. Two schemes for positron production are under study, one with e{sup -}/e{sup +} conversion at low energy (< 1 Gev) and one with conversion at 6 GeV and a recirculation line to bring the positrons back to the DR. Acceleration through the Linac is provided by a 2856 MHz RF system made of travelling wave (TW), room temperature accelerating structures.

  3. First results and planned experiments with the INFN-LNS ray-tracing magnetic spectrometer MAGNEX

    SciTech Connect

    Cunsolo, A.; Cappuzzello, F.; Cavallaro, M.; Orrigo, S. E. A.; Foti, A.; Rodrigues, M. R. D.; Borello-Lewin, T.; Petrascu, H.; Carbone, D.

    2010-05-21

    The MAGNEX large-acceptance ray-tracing magnetic spectrometer has recently been used with beams from the INFN-LNS Tandem accelerator. After an accurate commissioning, the instrument has started an ambitious experimental program. In the first experiment the {sup 19}F({sup 7}Li,{sup 7}Be){sup 19}O charge-exchange reaction was studied at 52 MeV incident energy. The {sup 19}O excitation energy spectrum was reconstructed and the angular distributions measured. The second experiment was aimed at the study of the {sup 15}C via the {sup 13}C({sup 18}O,{sup 16}O){sup 15}C reaction at 84 MeV incident energy. The ejectiles where detected at forward angles and mass identified by means of an innovative technique. The {sup 15}C excitation energy spectra up to about 20 MeV were obtained with a 250 keV FWHM energy resolution. In addition to several known states, the spectra show two unknown resonant-like structures at 11.4 and 14.0 MeV. The strong population of these structures, together with the measured widths, could indicate the collective nature of these states associated to a correlated neutron pair transfer. Besides the first results of physical interest from these two experiments, future experiments with MAGNEX are briefly outlined.

  4. The nuclear matrix elements of 0vββ decay and the NUMEN project at INFN-LNS

    NASA Astrophysics Data System (ADS)

    Cappuzzello, F.; Agodi, C.; Aciksoz, E.; Acosta, L.; Aslanouglou, X.; Auerbach, N.; Bijker, R.; Bonanno, D.; Bongiovanni, D.; Borello, T.; Boudhaim, S.; Bouhssa, M. L.; Boztosun, I.; Calabretta, L.; Calanna, A.; Carbone, D.; Cavallaro, M.; Calvo, D.; Chávez Lomelí, E. R.; Colonna, M.; D'Agostino, G.; Deshmukh, N.; de Faria, P. N.; Ferrero, A.; Foti, A.; Finocchiaro, P.; Gomes, P. R. S.; Greco, V.; Hacisalihoglu, A.; Housni, Z.; Khouaja, A.; Inchaou, J.; Lanzalone, G.; La Via, F.; Lay, J. A.; Lenske, H.; Linares, R.; Lubian, J.; Iazzi, F.; Introzzi, R.; Lavagno, A.; Lo Presti, D.; Medina, N.; Mendes, D. R.; Muoio, A.; Oliveira, J. R. B.; Pakou, A.; Pandola, L.; Rifuggiato, D.; Rodrigues, M. R. D.; Santagati, G.; Santopinto, E.; Scaltrito, L.; Sgouros, O.; Solakcı, S. O.; Soukeras, V.; Tudisco, S.; Vsevolodovna, R. I. M.; Zagatto, V.

    2016-07-01

    An innovative technique to access the nuclear matrix elements entering the expression of the life time of the double beta decay by relevant cross section measurements of double charge exchange reactions is proposed. A key aspect of the project is the use of the MAGNEX large acceptance magnetic spectrometer, for the detection of the ejectiles, and of the LNS K800 Superconducting Cyclotron (CS), for the acceleration of the required high resolution and low emittance heavy-ion beams, already in operation at INFN Laboratory Nazionali del Sud in Catania (Italy). However, a major upgrade is foreseen for the INFN-LNS research infrastructure to cope with beam currents as high as several ppA required by the project.

  5. Human Aldo-Keto Reductases: Function, Gene Regulation, and Single Nucleotide Polymorphisms

    PubMed Central

    Penning, Trevor M.; Drury, Jason E.

    2007-01-01

    Aldo-Keto Reductases (AKRs) are a superfamily of NAD(P)H linked oxidoreductases that are generally monomeric 34- 37 kDa proteins present in all phyla. The superfamily consists of 15 families, which contains 151 members (www.med.upenn.edu/akr). Thirteen human AKRs exist that use endogenous substrates (sugar and lipid aldehydes, prostaglandins, retinals and steroid hormones), and in many instances they regulate nuclear receptor signaling. Exogenous substrates include metabolites implicated in chemical carcinogenesis: NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone), polycyclic aromatic hydrocarbon trans-dihydrodiols, and aflatoxin dialdehyde. Promoter analysis of the human genes identifies common elements involved in their regulation which include osmotic response elements, antioxidant response elements, xenobiotic response elements, AP-1 sites and steroid response elements. The human AKRs are highly polymorphic, and in some instances single nucleotide polymorphisms (SNPs) of high penetrance exist. This suggests that there will be inter-individual variation in endogenous and xenobiotic metabolism which in turn affect susceptibility to nuclear receptor signaling and chemical carcinogenesis. PMID:17537398

  6. Roles of rat and human aldo-keto reductases in metabolism of farnesol and geranylgeraniol

    PubMed Central

    Endo, Satoshi; Matsunaga, Toshiyuki; Ohta, Chisato; Soda, Midori; Kanamori, Ayano; Kitade, Yukio; Ohno, Satoshi; Tajima, Kazuo; El-Kabbani, Ossama; Hara, Akira

    2011-01-01

    Farnesol (FOH) and geranylgeraniol (GGOH) with multiple biological actions are produced from the mevalonate pathway, and catabolized into farnesoic acid and geranylgeranoic acid, respectively, via the aldehyde intermediates (farnesal and geranylgeranial). We investigated the intracellular distribution, sequences and properties of the oxidoreductases responsible for the metabolic steps in rat tissues. The oxidation of FOH and GGOH into their aldehyde intermediates were mainly mediated by alcohol dehydrogenases 1 (in the liver and colon) and 7 (in the stomach and lung), and the subsequent step into the carboxylic acids was catalyzed by a microsomal aldehyde dehydrogenase. In addition, high reductase activity catalyzing the aldehyde intermediates into FOH (or GGOH) was detected in the cytosols of the extra-hepatic tissues, where the major reductase was identified as aldo-keto reductase (AKR) 1C15. Human reductases with similar specificity were identified as AKR1B10 and AKR1C3, which most efficiently reduced farnesal and geranylgeranial among seven enzymes in the AKR1A-1C subfamilies. The overall metabolism from FOH to farnesoic acid in cultured cells was significantly decreased by overexpression of AKR1C15, and increased by addition of AKR1C3 inhibitors, tolfenamic acid and R-flurbiprofen. Thus, AKRs (1C15 in rats, and 1B10 and 1C3 in humans) may play an important role in controlling the bioavailability of FOH and GGOH. PMID:21187079

  7. Dydrogesterone metabolism in human liver by aldo-keto reductases and cytochrome P450 enzymes.

    PubMed

    Olbrich, Matthias; Weigl, Kevin; Kahler, Elke; Mihara, Katsuhiro

    2016-10-01

    1. The metabolism of dydrogesterone was investigated in human liver cytosol (HLC) and human liver microsomes (HLM). Enzymes involved in dydrogesterone metabolism were identified and their relative contributions were estimated. 2. Dydrogesterone clearance was clearly higher in HLC compared to HLM. The major active metabolite 20α-dihydrodydrogesterone (20α-DHD) was only produced in HLC. 3. The formation of 20α-DHD by cytosolic aldo-keto reductase 1C (AKR1C) was confirmed with isoenzyme-specific AKR inhibitors. 4. Using recombinantly expressed human cytochrome P450 (CYP) isoenzymes, dydrogesterone was shown to be metabolically transformed by CYP3A4 and CYP2C19. 5. A clear contribution of CYP3A4 to microsomal metabolism of dydrogesterone was demonstrated with HLM and isoenzyme-specific CYP inhibitors, and confirmed by a significant correlation between dydrogesterone clearance and CYP3A4 activity. 6. Contribution of CYP2C19 was shown to be clearly less than CYP3A4 and restricted to a small group of human individuals with very high CYP2C19 activity. Therefore, it is expected that CYP2C19 genetic variations will not affect dydrogesterone pharmacokinetics in man. 7. In conclusion, dydrogesterone metabolism in the liver is dominated primarily by cytosolic enzymes (particularly AKR1C) and secondarily by CYP3A4, with the former exclusively responsible for 20α-DHD formation. PMID:26796435

  8. Activity improvement of a Kluyveromyces lactis aldo-keto reductase KlAKR via rational design.

    PubMed

    Luo, Xi; Wang, Ya-Jun; Shen, Wei; Zheng, Yu-Guo

    2016-04-20

    Optically pure t-butyl 6-cyano-(3R, 5R)-dihydroxyhexanoate ((R)-1b) is the key chiral precursor for atorvastatin calcium, the most widely used cholesterol-lowering drug. Wild-type aldo-keto reductase KlAKR from Kluyveromyces lactis has ideal diastereoselectivity toward t-butyl 6-cyano-(5R)-hydroxy-3-oxohexanoate (1a, dep>99.5%) but poor activity. A rational engineering was used to improve the KlAKR activity. Based on homology modeling and molecular docking, two amino acid residues (295 and 296) were selected as mutation sites, and two rounds of site-saturation mutagenesis were performed. Among the mutants, KlAKR-Y295W/W296L exhibited the highest catalytic efficiency (kcat/Km) toward 1a up to 12.37s(-1)mM(-1), which was 11.25-fold higher than that of wild-type KlAKR. Moreover, the majority of mutations have no negative impact on stereoselectivity. Using KlAKR-Y295W/W296L coupled with Exiguobacterium sibiricum glucose dehydrogenase (EsGDH) for cofactor regeneration, (R)-1b was accumulated up to 162.7mM with dep value above 99.5%. KlAKR-Y295W/W296L represents a robust tool for (R)-1b synthesis. PMID:26959479

  9. Use of 70 MeV Proton Beam for Medical Applications at INFN-LNS: CATANA Project

    SciTech Connect

    Sabini, M.G.; Cirrone, G.A.P.; Barone Tonghi, L.; Bartolotta, A.; Brai, M.; Cuttone, G.; Lo Nigro, S.; Marano, F.; Nicoletti, G.A.; Privitera, G.; Raffaele, L.; Reibaldi, A.; Romeo, N.; Rovelli, A.; Salamone, V.; Teri, G.

    2000-12-31

    The project CATANA (Centro di AdroTerapia ed Applicazioni Nucleari Avanzate) is a collaboration between the INFN-Laboratori Nazionali del Sud (LNS), Physics Department, Ophthalmology Institute and Radiology Institute of the Catania University and CSFNSM Catania. The main goal of CATANA is the study and the application of proton therapy for the treatment of shallow tumors (4 cm max) like uveal melanomas and subfoveal macular degenerations.

  10. A self-configuring control system for storage and computing departments at INFN-CNAF Tierl

    NASA Astrophysics Data System (ADS)

    Gregori, Daniele; Dal Pra, Stefano; Ricci, Pier Paolo; Pezzi, Michele; Prosperini, Andrea; Sapunenko, Vladimir

    2015-05-01

    The storage and farming departments at the INFN-CNAF Tier1[1] manage approximately thousands of computing nodes and several hundreds of servers that provides access to the disk and tape storage. In particular, the storage server machines should provide the following services: an efficient access to about 15 petabytes of disk space with different cluster of GPFS file system, the data transfers between LHC Tiers sites (Tier0, Tier1 and Tier2) via GridFTP cluster and Xrootd protocol and finally the writing and reading data operations on magnetic tape backend. One of the most important and essential point in order to get a reliable service is a control system that can warn if problems arise and which is able to perform automatic recovery operations in case of service interruptions or major failures. Moreover, during daily operations the configurations can change, i.e. if the GPFS cluster nodes roles can be modified and therefore the obsolete nodes must be removed from the control system production, and the new servers should be added to the ones that are already present. The manual management of all these changes is an operation that can be somewhat difficult in case of several changes, it can also take a long time and is easily subject to human error or misconfiguration. For these reasons we have developed a control system with the feature of self-configure itself if any change occurs. Currently, this system has been in production for about a year at the INFN-CNAF Tier1 with good results and hardly any major drawback. There are three major key points in this system. The first is a software configurator service (e.g. Quattor or Puppet) for the servers machines that we want to monitor with the control system; this service must ensure the presence of appropriate sensors and custom scripts on the nodes to check and should be able to install and update software packages on them. The second key element is a database containing information, according to a suitable format, on

  11. Structure and Promoter Characterization of Aldo-Keto Reductase Family 1 B10 Gene

    PubMed Central

    Liu, Ziwen; Zhong, Linlin; Krishack, Paulette A; Robbins, Sarah; Cao, Julia X; Zhao, Yupei; Chung, Stephen; Cao, Deliang

    2009-01-01

    Aldo-keto reductase family 1 member B10 (AKR1B10) is overexpressed in human hepatocellular carcinoma, lung squamous carcinoma, and lung adenocarcinoma in smokers. Our recent studies have showed that AKR1B10 plays a critical role in the growth and proliferation of cancer cells by detoxifying reactive carbonyls and regulating fatty acid biosynthesis. However, little is known about the regulatory mechanisms of AKR1B10 expression. In this study, we determined the structure of AKR1B10 gene and characterized its promoter. The results demonstrated that AKR1B10 consists of 10 exons and 9 introns, stretching approximately 13.8 kb. A 5′-RACE study determined the transcriptional start site of AKR1B10 at 320 bp upstream of the ATG translational start codon. A TATA-like (TAATAA) and a CAAT box are present from −145 to −140 bp and −193 to −190 bp upstream of the transcriptional start site, respectively. Motif analysis recognized multiple putative oncogenic and tumor suppressor protein binding sites in the AKR1B10 promoter, including c-Ets-1, C/EBP, AP-1, and p53, but osmolytic response elements were not found. A -4,091 bp of the 5′-flanking fragment of the AKR1B10 gene was capable of driving GFP and luciferase reporter gene expression in HepG2 cells derived from human hepatocellular carcinoma; progressive 5′-deletions revealed that a −255 bp fragment possesses full promoter activity. PMID:19236911

  12. Aldo-keto Reductase 1B15 (AKR1B15)

    PubMed Central

    Weber, Susanne; Salabei, Joshua K.; Möller, Gabriele; Kremmer, Elisabeth; Bhatnagar, Aruni; Adamski, Jerzy; Barski, Oleg A.

    2015-01-01

    Aldo-keto reductases (AKRs) comprise a superfamily of proteins involved in the reduction and oxidation of biogenic and xenobiotic carbonyls. In humans, at least 15 AKR superfamily members have been identified so far. One of these is a newly identified gene locus, AKR1B15, which clusters on chromosome 7 with the other human AKR1B subfamily members (i.e. AKR1B1 and AKR1B10). We show that alternative splicing of the AKR1B15 gene transcript gives rise to two protein isoforms with different N termini: AKR1B15.1 is a 316-amino acid protein with 91% amino acid identity to AKR1B10; AKR1B15.2 has a prolonged N terminus and consists of 344 amino acid residues. The two gene products differ in their expression level, subcellular localization, and activity. In contrast with other AKR enzymes, which are mostly cytosolic, AKR1B15.1 co-localizes with the mitochondria. Kinetic studies show that AKR1B15.1 is predominantly a reductive enzyme that catalyzes the reduction of androgens and estrogens with high positional selectivity (17β-hydroxysteroid dehydrogenase activity) as well as 3-keto-acyl-CoA conjugates and exhibits strong cofactor selectivity toward NADP(H). In accordance with its substrate spectrum, the enzyme is expressed at the highest levels in steroid-sensitive tissues, namely placenta, testis, and adipose tissue. Placental and adipose expression could be reproduced in the BeWo and SGBS cell lines, respectively. In contrast, AKR1B15.2 localizes to the cytosol and displays no enzymatic activity with the substrates tested. Collectively, these results demonstrate the existence of a novel catalytically active AKR, which is associated with mitochondria and expressed mainly in steroid-sensitive tissues. PMID:25577493

  13. Putative Role of the Aldo-Keto Reductase from Trypanosoma cruzi in Benznidazole Metabolism.

    PubMed

    Garavaglia, Patricia Andrea; Laverrière, Marc; Cannata, Joaquín J B; García, Gabriela Andrea

    2016-05-01

    Benznidazole (Bz), the drug used for treatment of Chagas' disease (caused by the protozoan Trypanosoma cruzi), is activated by a parasitic NADH-dependent type I nitroreductase (NTR I). However, several studies have shown that other enzymes are involved. The aim of this study was to evaluate whether the aldo-keto reductase from T. cruzi (TcAKR), a NADPH-dependent oxido-reductase previously described by our group, uses Bz as the substrate. We demonstrated that both recombinant and native TcAKR enzymes reduce Bz by using NADPH, but not NADH, as a cofactor. TcAKR-overexpressing epimastigotes showed higher NADPH-dependent Bz reductase activity and a 50% inhibitory concentration (IC50) value for Bz 1.8-fold higher than that of the controls, suggesting that TcAKR is involved in Bz detoxification instead of activation. To understand the role of TcAKR in Bz metabolism, we studied TcAKR expression and NADPH/NADH-dependent Bz reductase activities in two T. cruzi strains with differential susceptibility to Bz: CL Brener and Nicaragua. Taking into account the results obtained with TcAKR-overexpressing epimastigotes, we expected the more resistant strain, Nicaragua, to have higher TcAKR levels than CL Brener. However, the results were the opposite. CL Brener showed 2-fold higher TcAKR expression and 5.7-fold higher NADPH-Bz reduction than the Nicaragua strain. In addition, NADH-dependent Bz reductase activity, characteristic of NTR I, was also higher in CL Brener than in Nicaragua. We conclude that although TcAKR uses Bz as the substrate, TcAKR activity is not a determinant of Bz resistance in wild-type strains and may be overcome by other enzymes involved in Bz activation, such as NADPH- and NADH-dependent reductases. PMID:26856844

  14. Human Aldo-Keto Reductases and the Metabolic Activation of Polycyclic Aromatic Hydrocarbons

    PubMed Central

    2015-01-01

    Aldo-keto reductases (AKRs) are promiscuous NAD(P)(H) dependent oxidoreductases implicated in the metabolic activation of polycyclic aromatic hydrocarbons (PAH). These enzymes catalyze the oxidation of non-K-region trans-dihydrodiols to the corresponding o-quinones with the concomitant production of reactive oxygen species (ROS). The PAH o-quinones are Michael acceptors and can form adducts but are also redox-active and enter into futile redox cycles to amplify ROS formation. Evidence exists to support this metabolic pathway in humans. The human recombinant AKR1A1 and AKR1C1–AKR1C4 enzymes all catalyze the oxidation of PAH trans-dihydrodiols to PAH o-quinones. Many human AKRs also catalyze the NADPH-dependent reduction of the o-quinone products to air-sensitive catechols, exacerbating ROS formation. Moreover, this pathway of PAH activation occurs in a panel of human lung cell lines, resulting in the production of ROS and oxidative DNA damage in the form of 8-oxo-2′-deoxyguanosine. Using stable-isotope dilution liquid chromatography tandem mass spectrometry, this pathway of benzo[a]pyrene (B[a]P) metabolism was found to contribute equally with the diol-epoxide pathway to the activation of this human carcinogen in human lung cells. Evaluation of the mutagenicity of anti-B[a]P-diol epoxide with B[a]P-7,8-dione on p53 showed that the o-quinone produced by AKRs was the more potent mutagen, provided that it was permitted to redox cycle, and that the mutations observed were G to T transversions, reminiscent of those observed in human lung cancer. It is concluded that there is sufficient evidence to support the role of human AKRs in the metabolic activation of PAH in human lung cell lines and that they may contribute to the causation of human lung cancer. PMID:25279998

  15. 9,10-Phenanthrenequinone promotes secretion of pulmonary aldo-keto reductases with surfactant.

    PubMed

    Matsunaga, Toshiyuki; Haga, Mariko; Watanabe, Gou; Shinoda, Yuhki; Endo, Satoshi; Kajiwara, Yu; Tanaka, Hiroyuki; Inagaki, Naoki; El-Kabbani, Ossama; Hara, Akira

    2012-02-01

    9,10-Phenanthrenequinone (9,10-PQ), a major quinone in diesel exhaust particles, induces apoptosis via the generation of reactive oxygen species (ROS) because of 9,10-PQ redox cycling. We have found that intratracheal infusion of 9,10-PQ facilitates the secretion of surfactant into rat alveolus. In the cultured rat lung, treatment with 9,10-PQ results in an increase in a lower-density surfactant by ROS generation through redox cycling of the quinone. The surfactant contains aldo-keto reductase (AKR) 1C15, which reduces 9,10-PQ and the enzyme level in the surfactant increases on treatment with 9,10-PQ suggesting an involvement of AKR1C15 in the redox cycling of the quinone. In six human cell types (A549, MKN45, Caco2, Hela, Molt4 and U937) only type II epithelial A549 cells secrete three human AKR1C subfamily members (AKR1C1, AKR1C2 and AKR1C3) with the surfactant into the medium; this secretion is highly increased by 9,10-PQ treatment. Using in vitro enzyme inhibition analysis, we have identified AKR1C3 as the most abundantly secreted AKR1C member. The AKR1C enzymes in the medium efficiently reduce 9,10-PQ and initiate its redox cycling accompanied by ROS production. The exposure of A549 cells to 9,10-PQ provokes viability loss, which is significantly protected by the addition of the AKR1C3 inhibitor and antioxidant enzyme and by the removal of the surfactants from the culture medium. Thus, the AKR1C enzymes secreted in pulmonary surfactants probably participate in the toxic mechanism triggered by 9,10-PQ. PMID:22281686

  16. Preliminary Results of the NASA Beacon Receiver for Alphasat Aldo Paraboni TDP5 Propagation Experiment

    NASA Technical Reports Server (NTRS)

    Nessel, James; Morse, Jacquelynne; Zemba, Michael; Riva, Carlo; Luini, Lorenzo

    2014-01-01

    NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have initiated a joint propagation campaign within the framework of the Alphasat propagation experiment to characterize rain attenuation, scintillation, and gaseous absorption effects of the atmosphere in the 40 GHz band. NASA GRC has developed and installed a K/Q-band (20/40 GHz) beacon receiver at the POLIMI campus in Milan, Italy, which receives the 20/40 GHz signals broadcast from the Alphasat Aldo Paraboni TDP#5 beacon payload. The primary goal of these measurements is to develop a physical model to improve predictions of communications systems performance within the Q-band. Herein, we describe the design and preliminary performance of the NASA propagation terminal, which has been installed and operating in Milan since May 2014. The receiver is based upon a validated Fast Fourier Transform (FFT) I/Q digital design approach utilized in other operational NASA propagation terminals, but has been modified to employ power measurement via a frequency estimation technique and to coherently track and measure the amplitude of the 20/40 GHz beacon signals. The system consists of a 1.2-m K-band and a 0.6-m Qband Cassegrain reflector employing synchronous open-loop tracking to track the inclined orbit of the Alphasat satellite. An 8 Hz sampling rate is implemented to characterize scintillation effects, with a 1-Hz measurement bandwidth dynamic range of 45 dB. A weather station with an optical disdrometer is also installed to characterize rain drop size distribution for correlation with physical based models.

  17. Performance of the NASA Beacon Receiver for the Alphasat Aldo Paraboni TDP5 Propagation Experiment

    NASA Technical Reports Server (NTRS)

    Nessel, James; Morse, Jacquelynne; Zemba, Michael; Riva, Carlo; Luini, Lorenzo

    2015-01-01

    NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have initiated a joint propagation campaign within the framework of the Alphasat propagation experiment to characterize rain attenuation, scintillation, and gaseous absorption effects of the atmosphere in the 40 gigahertz band. NASA GRC has developed and installed a K/Q-band (20/40 gigahertz) beacon receiver at the POLIMI campus in Milan, Italy, which receives the 20/40 gigahertz signals broadcast from the Alphasat Aldo Paraboni Technology Demonstration Payload (TDP) no. 5 beacon payload. The primary goal of these measurements is to develop a physical model to improve predictions of communications systems performance within the Q-band. Herein, we describe the design and preliminary performance of the NASA propagation terminal, which has been installed and operating in Milan since June 2014. The receiver is based upon a validated Fast Fourier Transform (FFT) I/Q digital design approach utilized in other operational NASA propagation terminals, but has been modified to employ power measurement via a frequency estimation technique and to coherently track and measure the amplitude of the 20/40 gigahertz beacon signals. The system consists of a 1.2-meter K-band and a 0.6-meter Q-band Cassegrain reflector employing synchronous open-loop tracking to track the inclined orbit of the Alphasat satellite. An 8 hertz sampling rate is implemented to characterize scintillation effects, with a 1-hertz measurement bandwidth dynamic range of 45 decibels. A weather station with an optical disdrometer is also installed to characterize rain drop size distribution for correlation with physical based models.

  18. Human aldo-keto reductases and the metabolic activation of polycyclic aromatic hydrocarbons.

    PubMed

    Penning, Trevor M

    2014-11-17

    Aldo-keto reductases (AKRs) are promiscuous NAD(P)(H) dependent oxidoreductases implicated in the metabolic activation of polycyclic aromatic hydrocarbons (PAH). These enzymes catalyze the oxidation of non-K-region trans-dihydrodiols to the corresponding o-quinones with the concomitant production of reactive oxygen species (ROS). The PAH o-quinones are Michael acceptors and can form adducts but are also redox-active and enter into futile redox cycles to amplify ROS formation. Evidence exists to support this metabolic pathway in humans. The human recombinant AKR1A1 and AKR1C1-AKR1C4 enzymes all catalyze the oxidation of PAH trans-dihydrodiols to PAH o-quinones. Many human AKRs also catalyze the NADPH-dependent reduction of the o-quinone products to air-sensitive catechols, exacerbating ROS formation. Moreover, this pathway of PAH activation occurs in a panel of human lung cell lines, resulting in the production of ROS and oxidative DNA damage in the form of 8-oxo-2'-deoxyguanosine. Using stable-isotope dilution liquid chromatography tandem mass spectrometry, this pathway of benzo[a]pyrene (B[a]P) metabolism was found to contribute equally with the diol-epoxide pathway to the activation of this human carcinogen in human lung cells. Evaluation of the mutagenicity of anti-B[a]P-diol epoxide with B[a]P-7,8-dione on p53 showed that the o-quinone produced by AKRs was the more potent mutagen, provided that it was permitted to redox cycle, and that the mutations observed were G to T transversions, reminiscent of those observed in human lung cancer. It is concluded that there is sufficient evidence to support the role of human AKRs in the metabolic activation of PAH in human lung cell lines and that they may contribute to the causation of human lung cancer. PMID:25279998

  19. Applications of radiocarbon measurements in environmental studies at INFN-LABEC, Florence

    NASA Astrophysics Data System (ADS)

    Fedi, M.; Álvarez-Iglesias, P.; Caforio, L.; Calzolai, G.; Bernardoni, V.; Chiari, M.; Nava, S.; Taccetti, F.; Vecchi, R.

    2012-04-01

    be contaminated by heterogeneous materials, including organic matter too. Pre-treatment should thus remove all the possible contaminations without losing too much mass of the samples. Here we present an overview of the environmental radiocarbon applications the INFN-LABEC laboratory in Florence is involved in. 14C is measured by AMS, using the dedicated beam line installed at the 3 MV Tandem accelerator. In particular, details about the hardware and the experimental procedures are given.

  20. The charge breeder beam line for the selective production of exotic species project at INFN-Legnaro National Laboratories

    NASA Astrophysics Data System (ADS)

    Galatà, A.; Comunian, M.; Maggiore, M.; Manzolaro, M.; Angot, J.; Lamy, T.

    2014-02-01

    SPES (Selective Production of Exotic Species) is an INFN (Istituto Nazionale di Fisica Nucleare) project with the aim at producing and post-accelerating exotic beams to perform forefront research in nuclear physics. To allow post-acceleration of the radioactive ions, an ECR-based Charge Breeder (CB) developed on the basis of the Phoenix booster was chosen. The design of the complete beam line for the SPES-CB will be described: a system for stable 1+ beams production was included; special attention was paid to the medium resolution mass spectrometer after the CB to limit possible superposition of the exotic beams with the impurities present in the ECR plasma.

  1. Inter-comparison of boron concentration measurements at INFN-University of Pavia (Italy) and CNEA (Argentina).

    PubMed

    Portu, Agustina; Postuma, Ian; Gadan, Mario Alberto; Saint Martin, Gisela; Olivera, María Silvina; Altieri, Saverio; Protti, Nicoletta; Bortolussi, Silva

    2015-11-01

    An inter-comparison of three boron determination techniques was carried out between laboratories from INFN-University of Pavia (Italy) and CNEA (Argentina): alpha spectrometry (alpha-spect), neutron capture radiography (NCR) and quantitative autoradiography (QTA). Samples of different nature were analysed: liquid standards, liver homogenates and tissue samples from different treatment protocols. The techniques showed a good agreement in a concentration range of interest in BNCT (1-100ppm), thus demonstrating their applicability as precise methods to quantify boron and determine its distribution in tissues. PMID:26454177

  2. Reprint of Inter-comparison of boron concentration measurements at INFN-University of Pavia (Italy) and CNEA (Argentina).

    PubMed

    Portu, Agustina; Postuma, Ian; Gadan, Mario Alberto; Saint Martin, Gisela; Olivera, María Silvina; Altieri, Saverio; Protti, Nicoletta; Bortolussi, Silva

    2015-12-01

    An inter-comparison of three boron determination techniques was carried out between laboratories from INFN-University of Pavia (Italy) and CNEA (Argentina): alpha spectrometry (alpha-spect), neutron capture radiography (NCR) and quantitative autoradiography (QTA). Samples of different nature were analysed: liquid standards, liver homogenates and tissue samples from different treatment protocols. The techniques showed a good agreement in a concentration range of interest in BNCT (1-100 ppm), thus demonstrating their applicability as precise methods to quantify boron and determine its distribution in tissues. PMID:26508276

  3. The nuclear matrix elements of 0νββ decay and the NUMEN project at INFN-LNS

    NASA Astrophysics Data System (ADS)

    Cappuzzello, F.; Agodi, C.; Balestra, F.; Bijker, R.; Bonanno, D.; Bongiovanni, D.; Branchina, V.; Calabrese, S.; Calabretta, L.; Calanna, A.; Calvo, D.; Carbone, D.; Cavallaro, M.; Colonna, M.; Ferrero, S.; Foti, A.; Finocchiaro, P.; Giraudo, G.; Greco, V.; Iazzi, F.; Introzzi, R.; Lanzalone, G.; Lavagno, A.; Lo Presti, D.; Longhitano, F.; Muoio, A.; Pandola, L.; Rifuggiato, D.; Ruslan, M. V.; Santopinto, E.; Scaltrito, L.; Tudisco, S.; Zagatto, V.

    2016-05-01

    An innovative technique to access the nuclear matrix elements entering the expression of the life time of the double beta decay by relevant cross sections measurements of double charge exchange reactions is proposed. A key aspect of the project is the use of the MAGNEX large acceptance magnetic spectrometer, for the detection of the ejectiles, and of the LNS K800 Superconducting Cyclotron (CS), for the acceleration of the required high resolution and low emittance heavyion beams, already in operation at INFN Laboratory Nazionali del Sud in Catania (Italy).

  4. Substrate specificity and catalytic efficiency of aldo-keto reductases with phospholipid aldehydes

    PubMed Central

    Spite, Matthew; Baba, Shahid P.; Ahmed, Yonis; Barski, Oleg A.; Nijhawan, Kanchan; Petrash, J. Mark; Bhatnagar, Aruni; Srivastava, Sanjay

    2007-01-01

    Phospholipid oxidation generates several bioactive aldehydes that remain esterified to the glycerol backbone (‘core’ aldehydes). These aldehydes induce endothelial cells to produce monocyte chemotactic factors and enhance monocyte–endothelium adhesion. They also serve as ligands of scavenger receptors for the uptake of oxidized lipoproteins or apoptotic cells. The biochemical pathways involved in phospholipid aldehyde metabolism, however, remain largely unknown. In the present study, we have examined the efficacy of the three mammalian AKR (aldo-keto reductase) families in catalysing the reduction of phospholipid aldehydes. The model phospholipid aldehyde POVPC [1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine] was efficiently reduced by members of the AKR1, but not by the AKR6 or the ARK7 family. In the AKR1 family, POVPC reductase activity was limited to AKR1A and B. No significant activity was observed with AKR1C enzymes. Among the active proteins, human AR (aldose reductase) (AKR1B1) showed the highest catalytic activity. The catalytic efficiency of human small intestinal AR (AKR1B10) was comparable with the murine AKR1B proteins 1B3 and 1B8. Among the murine proteins AKR1A4 and AKR1B7 showed appreciably lower catalytic activity as compared with 1B3 and 1B8. The human AKRs, 1B1 and 1B10, and the murine proteins, 1B3 and 1B8, also reduced C-7 and C-9 sn-2 aldehydes as well as POVPE [1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphoethanolamine]. AKR1A4, B1, B7 and B8 catalysed the reduction of aldehydes generated in oxidized C16:0-20:4 phosphatidylcholine with acyl, plasmenyl or alkyl linkage at the sn-1 position or C16:0-20:4 phosphatidylglycerol or phosphatidic acid. AKR1B1 displayed the highest activity with phosphatidic acids; AKR1A4 was more efficient with long-chain aldehydes such as 5-hydroxy-8-oxo-6-octenoyl derivatives, whereas AKR1B8 preferred phosphatidylglycerol. These results suggest that proteins of the AKR1A and B families are

  5. Characterization and identification of three novel aldo-keto reductases from Lodderomyces elongisporus for reducing ethyl 4-chloroacetoacetate.

    PubMed

    Ning, Chenxi; Su, Erzheng; Wei, Dongzhi

    2014-12-15

    Lodderomyces elongisporus LH703 isolated from soil samples contained three novel aldo-keto reductases (AKRs) (LEAKR 48, LEAKR 49, and LEAKR 50). The three enzymes were cloned, expressed, and purified to homogeneity for characterization. These three AKRs shared <40% amino acid identity with each other. LEAKR 50 was identified as a member of AKR3 family, whereas the other two LEAKRs were identified as members of two novel AKR families, respectively. All the three AKRs required nicotinamide adenine dinucleotide phosphate as a cofactor. However, they showed diverse characteristics, including optimum catalyzing conditions, resistance to adverse reaction conditions, and substrate specificity. LEAKR 50 was estimated to be a promising biocatalyst that could reduce ethyl 4-chloroacetoacetate with high enantiomeric excess (98% e. e.) and high activity residue under adverse conditions. PMID:25447817

  6. A potassium channel beta subunit related to the aldo-keto reductase superfamily is encoded by the Drosophila hyperkinetic locus.

    PubMed

    Chouinard, S W; Wilson, G F; Schlimgen, A K; Ganetzky, B

    1995-07-18

    Genetic and physiological studies of the Drosophila Hyperkinetic (Hk) mutant revealed defects in the function or regulation of K+ channels encoded by the Shaker (Sh) locus. The Hk polypeptide, determined from analysis of cDNA clones, is a homologue of mammalian K+ channel beta subunits (Kv beta). Coexpression of Hk with Sh in Xenopus oocytes increases current amplitudes and changes the voltage dependence and kinetics of activation and inactivation, consistent with predicted functions of Hk in vivo. Sequence alignments show that Hk, together with mammalian Kv beta, represents an additional branch of the aldo-keto reductase superfamily. These results are relevant to understanding the function and evolutionary origin of Kv beta. PMID:7542775

  7. Pyrithione-based ruthenium complexes as inhibitors of aldo-keto reductase 1C enzymes and anticancer agents.

    PubMed

    Kljun, Jakob; Anko, Maja; Traven, Katja; Sinreih, Maša; Pavlič, Renata; Peršič, Špela; Ude, Žiga; Codina, Elisa Esteve; Stojan, Jure; Lanišnik Rižner, Tea; Turel, Iztok

    2016-08-01

    Four ruthenium complexes of clinically used zinc ionophore pyrithione and its oxygen analog 2-hydroxypyridine N-oxide were prepared and evaluated as inhibitors of enzymes of the aldo-keto reductase subfamily 1C (AKR1C). A kinetic study assisted with docking simulations showed a mixed type of inhibition consisting of a fast reversible and a slow irreversible step in the case of both organometallic compounds 1A and 1B. Both compounds also showed a remarkable selectivity towards AKR1C1 and AKR1C3 which are targets for breast cancer drug design. The organoruthenium complex of ligand pyrithione as well as pyrithione itself also displayed toxicity on the hormone-dependent MCF-7 breast cancer cell line with EC50 values in the low micromolar range. PMID:27357845

  8. Aldo-keto reductase 1C subfamily genes in skin are UV-inducible: possible role in keratinocytes survival.

    PubMed

    Marín, Yarí E; Seiberg, Miri; Lin, Connie B

    2009-07-01

    Please cite this paper as: Aldo-keto reductase 1C subfamily genes in skin are UV-inducible: possible role in keratinocytes survival. Experimental Dermatology 2009; 18: 611-618.Abstract: Human skin is endowed with the capacity to synthesize and metabolize steroid hormones, a function of importance in skin physiology and pathology. It is the hormone-regulatory enzymes, including the aldo-keto reductase 1C subfamily (AKR1Cs) that are largely responsible for the local levels of active steroid hormones. AKR1C1 and AKR1C2 inactivate progesterone and 5alpha-dihydrotestosterone, respectively, whereas AKR1C3 activates oestradiol and testosterone. Here, we show that AKR1C1-3 are expressed in keratinocytes and fibroblasts, with marginal expression in melanocytes. In human primary keratinocytes, AKR1C1 and -2 were UVB-inducible in a dose-dependent manner, as shown by quantitative PCR and Western blot analyses. The induction of AKR1C1 by UVB was concomitant with the presence of an apoptotic marker, the cleavage product of poly-ADP ribose polymerase. Similarly, the activation of AKR1C1 and -2 upon UVB exposure was demonstrated in swine skin in vivo and in human skin explants. As expected, hydrogen peroxide-derived reactive oxygen species also induced AKR1C1 and -2 mRNA and protein levels in keratinocytes in a dose-dependent manner. Furthermore, down-regulation of AKR1Cs by small interfering ribonucleic acid led to significantly reduced cell viability. Based on the combined evidence of the presence of an apoptotic marker in the UVB-exposed keratinocytes with increased AKR1Cs expression and reduced cell viability in down-regulated AKR1Cs, we suggest that AKR1C subfamily genes are stress-inducible and might function as survival factors in keratinocytes. PMID:19320734

  9. Wide area scanning system and carbon microbeams at the external microbeam facility of the INFN LABEC laboratory in Florence

    NASA Astrophysics Data System (ADS)

    Giuntini, L.; Massi, M.; Calusi, S.; Castelli, L.; Carraresi, L.; Fedi, M. E.; Gelli, N.; Liccioli, L.; Mandò, P. A.; Mazzinghi, A.; Palla, L.; Romano, F. P.; Ruberto, C.; Taccetti, F.

    2015-04-01

    Recently, developments have been made to the external scanning microbeam of INFN-LABEC laboratory in Florence. A new system for mechanical sample scanning was implemented. This system allows us to acquire large maps (up to 20 × 20 cm2), of great interest in the Cultural Heritage field. In parallel, the possibility of using carbon microbeams for experiments, such as, for example, ion beam modification of materials and MeV Secondary Ion Mass Spectrometry, has been investigated. As a test application, Particle Induced X-ray Emission with carbon microbeams has been performed on a lapis lazuli stone. First results for both wide area imaging and external carbon microbeams are briefly reported.

  10. Status of the SPES-charge breeder (SPES-CB) and its beam line at INFN-LNL

    NASA Astrophysics Data System (ADS)

    Galatá, Àlessio; Comunian, M.; Bellan, L.; Maggiore, M.; Patti, G.; Roncolato, C.; Bisoffi, G.; Russo, A. D.; Calabretta, L.; Angot, J.; Lamy, T.

    2016-06-01

    The Selective Production of Exotic Species (SPES) facility is under construction at INFN-LNL: aim of this project is the production, ionization and post-acceleration of radioactive ions to perform forefront research in nuclear physics. Radioactive species will be produced by fissions induced by a proton beam impinging on an UCx target: the proton beam will be delivered by a room temperature cyclotron (built by the Best Company) with a maximum energy of 40 MeV and 0.25 mA of maximum current. The radioactive species produced in the Target-Ion-Source system, extracted as a 1+ beam, cooled in a RFQ-cooler and purified from the isobars contaminants through a High Resolution Mass Spectrometer (HRMS). In order to allow post acceleration with the superconducting linac ALPI at INFN-LNL (up to 10 MeV/A for A/q = 7), an ECR-based charge breeding technique (ECR-CB) was chosen: in particular the SPES-CB was developed by the LPSC Grenoble on the basis of the Phoenix booster. The SPES-CB will be equipped with a complete test bench, totally integrated with the SPES beam line: in particular, in order to avoid beam contaminations induced by the impurities present inside the SPES-CB, and to have high transmission for a beam of very low intensity, special attention was paid not only to the transport efficiency but also to the resolution of the spectrometer downstream the charge breeder, with the design of a Medium Resolution Mass Spectrometer (MRMS). In the following paper the technical aspects connected with SPES-CB, its beam line and the transport of highly charged radioactive ions will be described.

  11. ALDO: A radiation-tolerant, low-noise, adjustable low drop-out linear regulator in 0.35 μm CMOS technology

    NASA Astrophysics Data System (ADS)

    Carniti, P.; Cassina, L.; Gotti, C.; Maino, M.; Pessina, G.

    2016-07-01

    In this work we present ALDO, an adjustable low drop-out linear regulator designed in AMS 0.35 μm CMOS technology. It is specifically tailored for use in the upgraded LHCb RICH detector in order to improve the power supply noise for the front end readout chip (CLARO). ALDO is designed with radiation-tolerant solutions such as an all-MOS band-gap voltage reference and layout techniques aiming to make it able to operate in harsh environments like High Energy Physics accelerators. It is capable of driving up to 200 mA while keeping an adequate power supply filtering capability in a very wide frequency range from 10 Hz up to 100 MHz. This property allows us to suppress the noise and high frequency spikes that could be generated by a DC/DC regulator, for example. ALDO also shows a very low noise of 11.6 μV RMS in the same frequency range. Its output is protected with over-current and short detection circuits for a safe integration in tightly packed environments. Design solutions and measurements of the first prototype are presented.

  12. The new ECR charge breeder for the Selective Production of Exotic Species project at INFN--Laboratori Nazionali di Legnaro.

    PubMed

    Galatà, A; Patti, G; Roncolato, C; Angot, J; Lamy, T

    2016-02-01

    The Selective Production of Exotic Species (SPES) project is an ISOL facility under construction at Istituto Nazionale di Fisica Nucleare-Laboratori Nationali di Legnaro (INFN-LNL). 1+ radioactive ions, produced and extracted from the target-ion-source system, will be charge bred to high charge states by an ECR charge breeder (SPES-CB): the project will adopt an upgraded version of the PHOENIX charge breeder, developed since about twenty years by the Laboratoire de Physique Subatomique et de Cosmologie (LPSC). The collaboration between LNL and LPSC started in 2010 with charge breeding experiments performed on the LPSC test bench and led, in June 2014, to the signature of a Research Collaboration Agreement for the delivery of a complete charge breeder and ancillaries, satisfying the SPES requirements. Important technological aspects were tackled during the construction phase, as, for example, beam purity issues, electrodes alignment, and vacuum sealing. This phase was completed in spring 2015, after which the qualification tests were carried out at LPSC on the 1+/q+ test stand. This paper describes the characteristics of the SPES-CB, with particular emphasis on the results obtained during the qualification tests: charge breeding of Ar, Xe, Rb, and Cs satisfied the SPES requirements for different intensities of the injected 1+ beam, showing very good performances, some of which are "best ever" for this device. PMID:26932056

  13. A pathogenesis related-10 protein CaARP functions as aldo/keto reductase to scavenge cytotoxic aldehydes.

    PubMed

    Jain, Deepti; Khandal, Hitaishi; Khurana, Jitendra Paul; Chattopadhyay, Debasis

    2016-01-01

    Pathogenesis related-10 (PR-10) proteins are present as multigene family in most of the higher plants. The role of PR-10 proteins in plant is poorly understood. A sequence analysis revealed that a large number of PR-10 proteins possess conserved motifs found in aldo/keto reductases (AKRs) of yeast and fungi. We took three PR-10 proteins, CaARP from chickpea, ABR17 from pea and the major pollen allergen Bet v1 from silver birch as examples and showed that these purified recombinant proteins possessed AKR activity using various cytotoxic aldehydes including methylglyoxal and malondialdehyde as substrates and the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) as co-factor. Essential amino acids for this catalytic activity were identified by substitution with other amino acids. CaARP was able to discriminate between the reduced and oxidized forms of NADP independently of its catalytic activity and underwent structural change upon binding with NADPH. CaARP protein was preferentially localized in cytosol. When expressed in bacteria, yeast or plant, catalytically active variants of CaARP conferred tolerance to salinity, oxidative stress or cytotoxic aldehydes. CaARP-expressing plants showed lower lipid peroxidation product content in presence or absence of stress suggesting that the protein functions as a scavenger of cytotoxic aldehydes produced by metabolism and lipid peroxidation. Our result proposes a new biochemical property of a PR-10 protein. PMID:26577640

  14. Structural characterization of an aldo-keto reductase (AKR2E5) from the silkworm Bombyx mori.

    PubMed

    Yamamoto, Kohji; Higashiura, Akifumi; Suzuki, Mamoru; Shiotsuki, Takahiro; Sugahara, Ryohei; Fujii, Takeshi; Nakagawa, Atsushi

    2016-05-20

    We report a new member of the aldo-keto reductase (AKR) superfamily in the silkworm Bombyx mori. Based on its amino acid sequence, the new enzyme belongs to the AKR2 family and was previously assigned the systematic name AKR2E5. In the present study, recombinant AKR2E5 was expressed, purified to homogeneity, and characterized. The X-ray crystal structures were determined at 2.2 Å for the apoenzyme and at 2.3 Å resolution for the NADPH-AKR2E5 complex. Our results demonstrate that AKR2E5 is a 40-kDa monomer and includes the TIM- or (β/α)8-barrel typical for other AKRs. We found that AKR2E5 uses NADPH as a cosubstrate to reduce carbonyl compounds such as DL-glyceraldehyde, xylose, 3-hydroxy benzaldehyde, 17α-hydroxy progesterone, 11-hexadecenal, and bombykal. No NADH-dependent activity was detected. Site-directed mutagenesis of AKR2E5 indicates that amino acid residues Asp70, Tyr75, Lys104, and His137 contribute to catalytic activity, which is consistent with the data on other AKRs. To the best of our knowledge, AKR2E5 is only the second AKR characterized in silkworm. Our data should contribute to further understanding of the functional activity of insect AKRs. PMID:27103441

  15. Gene expression and promoter analysis of a novel tomato aldo-keto reductase in response to environmental stresses.

    PubMed

    Suekawa, Marina; Fujikawa, Yukichi; Inada, Shuhei; Murano, Asako; Esaka, Muneharu

    2016-08-01

    The functional role of an uncharacterized tomato (Solanum lycopersicum) aldo-keto reductase 4B, denoted as SlAKR4B, was investigated. The gene expression of tomato SlAKR4B was detected at a high level in the senescent leaves and the ripening fruits of tomato. Although d-galacturonic acid reductase activities tended to be higher in tomato SlAKR4B-overexpressing transgenic tobacco BY-2 cell lines than those in control cell lines, SlAKR4B gene expression was not well correlated with l-ascorbic acid content among the cell lines. The analysis of the transgenic cell lines showed that tomato SlAKR4B has enzyme activities toward d-galacturonic acid as well as glyceraldehyde and glyoxal, suggesting that the SlAKR4B gene encodes a functional enzyme in tomato. Gene expression of SlAKR4B was induced by NaCl, H2O2, and plant hormones such as salicylic acid and jasmonic acid, suggesting that SlAKR4B is involved in the stress response. The transient expression assay using protoplasts showed the promoter activity of the SlAKR4B gene was as high as that of the cauliflower mosaic virus 35S promoter. Also, the promoter region of the SlAKR4B gene was suggested to contain cis-element(s) for abiotic stress-inducible expression. PMID:27337067

  16. Disruption of aldo-keto reductase genes leads to elevated markers of oxidative stress and inositol auxotrophy in Saccharomyces cerevisiae

    PubMed Central

    Chang, Qing; Mark Petrash, J.

    2008-01-01

    A large family of aldo-keto reductases with similar kinetic and structural properties but unknown physiological roles is expressed in the yeast Saccharomyces cerevisiae. Strains with one or two AKR genes disrupted have apparently normal phenotypes, but disruption of at least three AKR genes results in a heat shock phenotype and slow growth in inositol-deficient culture medium (Ino-). The present study was carried out to identify metabolic or signaling defects that may underlie phenotypes that emerge in AKR deficient strains. Here we demonstrate that pre-treatment of a pentuple AKR null mutant with the anti-oxidative agent N-acetyl-cysteine rescues the heat shock phenotype. This indicates that AKR gene disruption may be associated with defects in oxidative stress response. We observed additional markers of oxidative stress in AKR-deficient strains, including reduced glutathione levels, constitutive nuclear localization of the oxidation-sensitive transcription factor Yap1 and up regulation of a set of Yap1 target genes whose function as a group is primarily involved in response to oxidative stress and redox balance. Genetic analysis of the Ino- phenotype of the null mutants showed that defects in transcriptional regulation of the INO1, which encodes for inositol-1-phosphate synthase, can be rescued through ectopic expression of a functional INO1. Taken together, these results suggest potential roles for AKRs in oxidative defense and transcriptional regulation. PMID:17919749

  17. Aldo Leopold's land health from a resilience point of view: self-renewal capacity of social-ecological systems.

    PubMed

    Berkes, Fikret; Doubleday, Nancy C; Cumming, Graeme S

    2012-09-01

    Health approaches to ecology have a strong basis in Aldo Leopold's thinking, and contemporary ecohealth in turn has a strong philosophical basis in Leopold. To commemorate the 125th anniversary of Leopold's birth (1887-1948), we revisit his ideas, specifically the notions of stewardship (land ethic), productive use of ecosystems (land), and ecosystem renewal. We focus on Leopold's perspective on the self-renewal capacity of the land, as understood in terms of integrity and land health, from the contemporary perspective of resilience theory and ecological theory more generally. Using a broad range of literature, we explore insights and implications of Leopold's work for today's human-environment relationships (integrated social-ecological systems), concerns for biodiversity, the development of agency with respect to stewardship, and key challenges of his time and of ours. Leopold's seminal concept of land health can be seen as a triangulation of productive use, self-renewal, and stewardship, and it can be reinterpreted through the resilience lens as the health of social-ecological systems. In contemporary language, this involves the maintenance of biodiversity and ecosystem services, and the ability to exercise agency both for conservation and for environmental justice. PMID:22968329

  18. Aldo Keto Reductase 1B7 and Prostaglandin F2α Are Regulators of Adrenal Endocrine Functions

    PubMed Central

    Lambert-Langlais, Sarah; Volat, Fanny; Manin, Michèle; Coudoré, François; Val, Pierre; Sahut-Barnola, Isabelle; Ragazzon, Bruno; Louiset, Estelle; Delarue, Catherine; Lefebvre, Hervé; Urade, Yoshihiro; Martinez, Antoine

    2009-01-01

    Prostaglandin F2α (PGF2α), represses ovarian steroidogenesis and initiates parturition in mammals but its impact on adrenal gland is unknown. Prostaglandins biosynthesis depends on the sequential action of upstream cyclooxygenases (COX) and terminal synthases but no PGF2α synthases (PGFS) were functionally identified in mammalian cells. In vitro, the most efficient mammalian PGFS belong to aldo-keto reductase 1B (AKR1B) family. The adrenal gland is a major site of AKR1B expression in both human (AKR1B1) and mouse (AKR1B3, AKR1B7). Thus, we examined the PGF2α biosynthetic pathway and its functional impact on both cortical and medullary zones. Both compartments produced PGF2α but expressed different biosynthetic isozymes. In chromaffin cells, PGF2α secretion appeared constitutive and correlated to continuous expression of COX1 and AKR1B3. In steroidogenic cells, PGF2α secretion was stimulated by adrenocorticotropic hormone (ACTH) and correlated to ACTH-responsiveness of both COX2 and AKR1B7/B1. The pivotal role of AKR1B7 in ACTH-induced PGF2α release and functional coupling with COX2 was demonstrated using over- and down-expression in cell lines. PGF2α receptor was only detected in chromaffin cells, making medulla the primary target of PGF2α action. By comparing PGF2α-responsiveness of isolated cells and whole adrenal cultures, we demonstrated that PGF2α repressed glucocorticoid secretion by an indirect mechanism involving a decrease in catecholamine release which in turn decreased adrenal steroidogenesis. PGF2α may be regarded as a negative autocrine/paracrine regulator within a novel intra-adrenal feedback loop. The coordinated cell-specific regulation of COX2 and AKR1B7 ensures the generation of this stress-induced corticostatic signal. PMID:19809495

  19. Metabolism of the Synthetic Progestogen Norethynodrel by Human Ketosteroid Reductases of the Aldo-Keto Reductase Superfamily

    PubMed Central

    Jin, Yi; Duan, Ling; Chen, Mo; Penning, Trevor M; Kloosterboer, Helenius J.

    2012-01-01

    Human ketosteroid reductases of the aldo-keto reductase (AKR) superfamily, i.e. AKR1C1-4, are implicated in the biotransformation of synthetic steroid hormones. Norethynodrel (NOR, 17α-ethynyl-17β-hydroxy-estra-5(10)-en-3-one), the progestin component of the first marketed oral contraceptive, is known to undergo rapid and extensive metabolism to 3α- and 3β-hydroxy metabolites. The ability of the four human AKR1C enzymes to catalyze the metabolism of NOR has now been characterized. AKR1C1 and AKR1C2 almost exclusively converted NOR to 3β-hydroxy NOR, while AKR1C3 gave 3β-hydroxy NOR as the main product and AKR1C4 predominantly formed 3α-hydroxy NOR. Individual AKR1C enzymes also displayed distinct kinetic properties in the reaction of NOR. In contrast, norethindrone (NET), the Δ4-isomer of NOR and the most commonly used synthetic progestin, was not a substrate for the AKR1C enzymes. NOR is also structurally identical to the hormone replacement therapeutic tibolone (TIB), except TIB has a methyl group at the 7α-position. Product profiles and kinetic parameters for the reduction of NOR catalyzed by each individual AKR1C isoform were identical to those for the reduction of TIB catalyzed by the respective isoform. These data suggest that the presence of the 7α-methyl group has a minimal effect on the stereochemical outcome of the reaction and kinetic behavior of each enzyme. Results indicate a role of AKR1C in the hepatic and peripheral metabolism of NOR to 3α- and 3β-hydroxy NOR and provide insights into the differential pharmacological properties of NOR, NET and TIB. PMID:22210085

  20. MoonLIGHT, a Lunar Laser Ranging Retroreflector Array for the 21st Century, and the ASI-INFN Etrusco-2 project

    NASA Astrophysics Data System (ADS)

    Delle Monache, Giovanni O.; Dell'Agnello, S.; Currie, D.; Martini, M.; Vittori, R.; Cantone, C.; Boni, A.; Berardi, S.; Patrizi, G.; Maiello, M.; Tibuzzi, M.; Garattini, M.; Lops, C.; Ciocci, E.; Graziosi, C.; Bianco, G.; Intaglietta, N.

    2012-05-01

    Since the 1970s Lunar Laser Ranging (LLR) to the Apollo Cube Corner Retroreflector (CCR) arrays supplied almost all significant tests of General Relativity and significant information on the composition and origin of the moon. In the 1970s Apollo LLR arrays contributed a negligible fraction of the ranging error budget. Since the ranging capabilities of ground stations improved by more than two orders of magnitude, now, because of the lunar librations, Apollo CCR arrays dominate the error budget. With the project MoonLIGHT in 2006 INFN-LNF joined UMD in the development and test of a new-generation LLR payload made by a single CCR unaffected by librations. In particular, INFN-LNF built and is operating a new experimental apparatus (SCF) and created a new industry-standard test procedure (SCF-Test) to characterize the thermal behavior and the optical performance of CCRs in simulated space conditions. Our key experimental innovation is the concurrent measurement and modeling of the optical FFDP and the temperature distribution of retroreflector payloads under thermal conditions produced with a close-match solar simulator. These capabilities provide: unique pre-launch performance validation of the space segment of LLR/SLR. Results of the SCF-Test of our CCR payload will be presented. Negotiations are underway to propose our payload and SCF-Test services for precision gravity and lunar science measurements with next robotic lunar landing missions. We will describe the addition of the CCR optical Wavefront Fizeau Interferogram (WFI) concurrently to FFDP/temperature measurements in the framework of an ASI-INFN project, ETRUSCO-2. The main goals of the latter are: development of a standard GNSS laser Retroreflector Array; a second SCF; SCF-Test of Galileo, GPS and other ‘as-built’ GNSS retroreflector payloads. Results on analysis of Apollo LLR data and search of new gravitational physics with LLR, Mercury Radar Ranging, SLR of LAGEOS (Laser GEOdynamics Satellite) will

  1. Expression, purification, crystallization and preliminary X-ray analysis of perakine reductase, a new member of the aldo-keto reductase enzyme superfamily from higher plants

    SciTech Connect

    Rosenthal, Cindy; Mueller, Uwe; Panjikar, Santosh; Sun, Lianli; Ruppert, Martin; Zhao, Yu; Stöckigt, Joachim

    2006-12-01

    Perakine reductase, a novel member of the aldo-keto reductase enzyme superfamily of higher plants, is involved in the biosynthesis of monoterpenoid indole alkaloids in the Indian medicinal plant Rauvolfia serpentina. The enzyme has been crystallized in C-centered orthorhombic space group and diffracts to 2.0 Å resolution. Perakine reductase (PR) is a novel member of the aldo-keto reductase enzyme superfamily from higher plants. PR from the plant Rauvolfia serpentina is involved in the biosynthesis of monoterpenoid indole alkaloids by performing NADPH-dependent reduction of perakine, yielding raucaffrinoline. However, PR can also reduce cinnamic aldehyde and some of its derivatives. After heterologous expression of a triple mutant of PR in Escherichia coli, crystals of the purified and methylated enzyme were obtained by the hanging-drop vapour-diffusion technique at 293 K with 100 mM sodium citrate pH 5.6 and 27% PEG 4000 as precipitant. Crystals belong to space group C222{sub 1} and diffract to 2.0 Å, with unit-cell parameters a = 58.9, b = 93.0, c = 143.4 Å.

  2. The set-up for forward scattered particle detection at the external microbeam facility of the INFN-LABEC laboratory in Florence

    NASA Astrophysics Data System (ADS)

    Giuntini, L.; Massi, M.; Calusi, S.; Gelli, N.; Castelli, L.; Carraresi, L.; Czelusniak, C.; Fedi, M. E.; Gueli, A. M.; Liccioli, L.; Mandò, P. A.; Mazzinghi, A.; Palla, L.; Ruberto, C.; Taccetti, F.

    2015-04-01

    In the last few years some new implementations and upgrades have been made to the external scanning microbeam of INFN-LABEC laboratory in Florence, enriching the existing PIXE, PIGE, BS, IBIL set-up with complementary techniques, when possible allowing for simultaneous multi-technique analyses. We developed a system, compatible with the existing set-up, for the out-of-vacuum detection of the forward scattered particles. This system makes feasible the external-STIM (Scanning Transmission Ion Microscopy) and external-FS (Forward Scattering), now both available at our beamline. Test measurements are shortly presented.

  3. Anthracycline resistance mediated by reductive metabolism in cancer cells: The role of aldo-keto reductase 1C3

    SciTech Connect

    Hofman, Jakub; Malcekova, Beata; Skarka, Adam; Novotna, Eva; Wsol, Vladimir

    2014-08-01

    Pharmacokinetic drug resistance is a serious obstacle that emerges during cancer chemotherapy. In this study, we investigated the possible role of aldo-keto reductase 1C3 (AKR1C3) in the resistance of cancer cells to anthracyclines. First, the reducing activity of AKR1C3 toward anthracyclines was tested using incubations with a purified recombinant enzyme. Furthermore, the intracellular reduction of daunorubicin and idarubicin was examined by employing the transfection of A549, HeLa, MCF7 and HCT 116 cancer cells with an AKR1C3 encoding vector. To investigate the participation of AKR1C3 in anthracycline resistance, we conducted MTT cytotoxicity assays with these cells, and observed that AKR1C3 significantly contributes to the resistance of cancer cells to daunorubicin and idarubicin, whereas this resistance was reversible by the simultaneous administration of 2′-hydroxyflavanone, a specific AKR1C3 inhibitor. In the final part of our work, we tracked the changes in AKR1C3 expression after anthracycline exposure. Interestingly, a reciprocal correlation between the extent of induction and endogenous levels of AKR1C3 was recorded in particular cell lines. Therefore, we suggest that the induction of AKR1C3 following exposure to daunorubicin and idarubicin, which seems to be dependent on endogenous AKR1C3 expression, eventually might potentiate an intrinsic resistance given by the normal expression of AKR1C3. In conclusion, our data suggest a substantial impact of AKR1C3 on the metabolism of daunorubicin and idarubicin, which affects their pharmacokinetic and pharmacodynamic behavior. In addition, we demonstrate that the reduction of daunorubicin and idarubicin, which is catalyzed by AKR1C3, contributes to the resistance of cancer cells to anthracycline treatment. - Highlights: • Metabolism of anthracyclines by AKR1C3 was studied at enzyme and cellular levels. • Anthracycline resistance mediated by AKR1C3 was demonstrated in cancer cells. • Induction of AKR1C3

  4. Glucocorticoid-induced androgen inactivation by aldo-keto reductase 1C2 promotes adipogenesis in human preadipocytes.

    PubMed

    Veilleux, Alain; Côté, Julie-Anne; Blouin, Karine; Nadeau, Mélanie; Pelletier, Mélissa; Marceau, Picard; Laberge, Philippe Y; Luu-The, Van; Tchernof, André

    2012-04-15

    Adipogenesis and lipid storage in human adipose tissue are inhibited by androgens such as DHT. Inactivation of DHT to 3α-diol is stimulated by glucocorticoids in human preadipocytes. We sought to characterize glucocorticoid-induced androgen inactivation in human preadipocytes and to establish its role in the antiadipogenic action of DHT. Subcutaneous and omental primary preadipocyte cultures were established from fat samples obtained in subjects undergoing abdominal surgeries. Inactivation of DHT to 3α/β-diol for 24 h was measured in dexamethasone- or vehicle-treated cells. Specific downregulation of aldo-keto reductase 1C (AKR1C) enzymes in human preadipocytes was achieved using RNA interference. In whole adipose tissue sample, cortisol production was positively correlated with androgen inactivation in both subcutaneous and omental adipose tissue (P < 0.05). Maximal dexamethasone (1 μM) stimulation of DHT inactivation was higher in omental compared with subcutaneous fat from men as well as subcutaneous and omental fat from women (P < 0.05). A significant positive correlation was observed between BMI and maximal dexamethasone-induced DHT inactivation rates in subcutaneous and omental adipose tissue of men and women (r = 0.24, n = 26, P < 0.01). siRNA-induced downregulation of AKR1C2, but not AKR1C1 or AKR1C3, significantly reduced basal and glucocorticoid-induced androgen inactivation rates (P < 0.05). The inhibitory action of DHT on preadipocyte differentiation was potentiated following AKR1C2 but not AKR1C1 or AKR1C3 downregulation. Specifically, lipid accumulation, G3PDH activity, and FABP4 mRNA expression in differentiated preadipocytes exposed to DHT were reduced further upon AKR1C2 siRNA transfection. We conclude that glucocorticoid-induced androgen inactivation is mediated by AKR1C2 and is particularly effective in omental preadipocytes of obese men. The interplay between glucocorticoids and AKR1C2-dependent androgen inactivation may locally modulate

  5. The role of cytochromes p450 and aldo-keto reductases in prognosis of breast carcinoma patients.

    PubMed

    Hlaváč, Viktor; Brynychová, Veronika; Václavíková, Radka; Ehrlichová, Marie; Vrána, David; Pecha, Václav; Trnková, Markéta; Kodet, Roman; Mrhalová, Marcela; Kubáčková, Kateřina; Gatěk, Jiří; Vážan, Petr; Souček, Pavel

    2014-12-01

    Metabolism of anticancer drugs affects their antitumor effects. This study has investigated the associations of gene expression of enzymes metabolizing anticancer drugs with therapy response and survival of breast carcinoma patients. Gene expression of 13 aldo-keto reductases (AKRs), carbonyl reductase 1, and 10 cytochromes P450 (CYPs) was assessed using quantitative real-time polymerase chain reaction in tumors and paired adjacent nonneoplastic tissues from 68 posttreatment breast carcinoma patients. Eleven candidate genes were then evaluated in an independent series of 50 pretreatment patients. Protein expression of the most significant genes was confirmed by immunoblotting. AKR1A1 was significantly overexpressed and AKR1C1-4, KCNAB1, CYP2C19, CYP3A4, and CYP3A5 downregulated in tumors compared with control nonneoplastic tissues after correction for multiple testing. Significant association of CYP2B6 transcript levels in tumors with expression of hormonal receptors was found in the posttreatment set and replicated in the pretreatment set of patients. Significantly higher intratumoral levels of AKR1C1, AKR1C2, or CYP2W1 were found in responders to neoadjuvant chemotherapy compared with nonresponders. Patients with high AKR7A3 or CYP2B6 levels in the pretreatment set had significantly longer disease-free survival than patients with low levels. Protein products of AKR1C1, AKR1C2, AKR7A3, CYP3A4, and carbonyl reductase (CBR1) were found in tumors and those of AKR1C1, AKR7A3, and CBR1 correlated with their transcript levels. Small interfering RNA-directed knockdown of AKR1C2 or vector-mediated upregulation of CYP3A4 in MDA-MB-231 model cell line had no effect on cell proliferation after paclitaxel treatment in vitro. Prognostic and predictive roles of drug-metabolizing enzymes strikingly differ between posttreatment and pretreatment breast carcinoma patients. Mechanisms of action of AKR1C2, AKR7A3, CYP2B6, CYP3A4, and CBR1 should continue to be further followed in

  6. Characterization of the aldo-keto reductase 1C gene cluster on pig chromosome 10: possible associations with reproductive traits

    PubMed Central

    Nonneman, Dan J; Wise, Tommy H; Ford, J Joe; Kuehn, Larry A; Rohrer, Gary A

    2006-01-01

    Background The rate of pubertal development and weaning to estrus interval are correlated and affect reproductive efficiency of swine. Quantitative trait loci (QTL) for age of puberty, nipple number and ovulation rate have been identified in Meishan crosses on pig chromosome 10q (SSC10) near the telomere, which is homologous to human chromosome 10p15 and contains an aldo-keto reductase (AKR) gene cluster with at least six family members. AKRs are tissue-specific hydroxysteroid dehydrogenases that interconvert weak steroid hormones to their more potent counterparts and regulate processes involved in development, homeostasis and reproduction. Because of their location in the swine genome and their implication in reproductive physiology, this gene cluster was characterized and evaluated for effects on reproductive traits in swine. Results Screening the porcine CHORI-242 BAC library with a full-length AKR1C4 cDNA identified 7 positive clones and sample sequencing of 5 BAC clones revealed 5 distinct AKR1C genes (AKR1CL2 and AKR1C1 through 4), which mapped to 126–128 cM on SSC10. Using the IMpRH7000rad and IMNpRH212000rad radiation hybrid panels, these 5 genes mapped between microsatellite markers SWR67 and SW2067. Comparison of sequence data with the porcine BAC fingerprint map show that the cluster of genes resides in a 300 kb region. Twelve SNPs were genotyped in gilts observed for age at first estrus and ovulation rate from the F8 and F10 generations of one-quarter Meishan descendants of the USMARC resource population. Age at puberty, nipple number and ovulation rate data were analyzed for association with genotypes by MTDFREML using an animal model. One SNP, a phenylalanine to isoleucine substitution in AKR1C2, was associated with age of puberty (p = 0.07) and possibly ovulation rate (p = 0.102). Two SNP in AKR1C4 were significantly associated with nipple number (p ≤ 0.03) and another possibly associated with age at puberty (p = 0.09). Conclusion AKR1C genotypes

  7. Structure of xylose reductase bound to NAD+ and the basis for single and dual co-substrate specificity in family 2 aldo-keto reductases.

    PubMed Central

    Kavanagh, Kathryn L; Klimacek, Mario; Nidetzky, Bernd; Wilson, David K

    2003-01-01

    The co-ordinates reported have been submitted to the Protein Data Bank under accession number 1MI3. Xylose reductase (XR; AKR2B5) is an unusual member of aldo-keto reductase superfamily, because it is one of the few able to efficiently utilize both NADPH and NADH as co-substrates in converting xylose into xylitol. In order to better understand the basis for this dual specificity, we have determined the crystal structure of XR from the yeast Candida tenuis in complex with NAD(+) to 1.80 A resolution (where 1 A=0.1 nm) with a crystallographic R -factor of 18.3%. A comparison of the NAD(+)- and the previously determined NADP(+)-bound forms of XR reveals that XR has the ability to change the conformation of two loops. To accommodate both the presence and absence of the 2'-phosphate, the enzyme is able to adopt different conformations for several different side chains on these loops, including Asn(276), which makes alternative hydrogen-bonding interactions with the adenosine ribose. Also critical is the presence of Glu(227) on a short rigid helix, which makes hydrogen bonds to both the 2'- and 3'-hydroxy groups of the adenosine ribose. In addition to changes in hydrogen-bonding of the adenosine, the ribose unmistakably adopts a 3'- endo conformation rather than the 2'- endo conformation seen in the NADP(+)-bound form. These results underscore the importance of tight adenosine binding for efficient use of either NADH or NADPH as a co-substrate in aldo-keto reductases. The dual specificity found in XR is also an important consideration in designing a high-flux xylose metabolic pathway, which may be improved with an enzyme specific for NADH. PMID:12733986

  8. INFN, IT the GENIUS grid portal and the robot certificates to perform phylogenetic analysis on large scale: a success story from the International LIBI project

    NASA Astrophysics Data System (ADS)

    Barbera, Roberto; Donvit, Giacinto; Falzone, Alberto; Rocca, Giuseppe La; Maggi, Giorgio Pietro; Milanesi, Luciano; Vicarioicario, Saverio

    This paper depicts the solution proposed by INFN to allow users, not owning a personal digital certificate and therefore not belonging to any specific Virtual Organization (VO), to access Grid infrastructures via the GENIUS Grid portal enabled with robot certificates. Robot certificates, also known as portal certificates, are associated with a specific application that the user wants to share with the whole Grid community and have recently been introduced by the EUGridPMA (European Policy Management Authority for Grid Authentication) to perform automated tasks on Grids on behalf of users. They are proven to be extremely useful to automate grid service monitoring, data processing production, distributed data collection systems, etc. In this paper, robot certificates have been used to allow bioinformaticians involved in the Italian LIBI project to perform large scale phylogenetic analyses. The distributed environment set up in this work strongly simplify the grid access of occasional users and represents a valuable step forward to wide the communities of users.

  9. cDNA cloning, expression and activity of a second human aflatoxin B1-metabolizing member of the aldo-keto reductase superfamily, AKR7A3.

    PubMed

    Knight, L P; Primiano, T; Groopman, J D; Kensler, T W; Sutter, T R

    1999-07-01

    The aflatoxin B1 (AFB1) aldehyde metabolite of AFB1 may contribute to the cytotoxicity of this hepatocarcinogen via protein adduction. Aflatoxin B1 aldehyde reductases, specifically the NADPH-dependent aldo-keto reductases of rat (AKR7A1) and human (AKR7A2), are known to metabolize the AFB1 dihydrodiol by forming AFB1 dialcohol. Using a rat AKR7A1 cDNA, we isolated and characterized a distinct aldo-keto reductase (AKR7A3) from an adult human liver cDNA library. The deduced amino acid sequence of AKR7A3 shares 80 and 88% identity with rat AKR7A1 and human AKR7A2, respectively. Recombinant rat AKR7A1 and human AKR7A3 were expressed and purified from Escherichia coli as hexa-histidine tagged fusion proteins. These proteins catalyzed the reduction of several model carbonyl-containing substrates. The NADPH-dependent formation of AFB1 dialcohol by recombinant human AKR7A3 was confirmed by liquid chromatography coupled to electrospray ionization mass spectrometry. Rabbit polyclonal antibodies produced using recombinant rat AKR7A1 protein were shown to detect nanogram amounts of rat and human AKR7A protein. The amount of AKR7A-related protein in hepatic cytosols of 1, 2-dithiole-3-thione-treated rats was 18-fold greater than in cytosols from untreated animals. These antibodies detected AKR7A-related protein in normal human liver samples ranging from 0.3 to 0.8 microg/mg cytosolic protein. Northern blot analysis showed varying levels of expression of AKR7A RNA in human liver and in several extrahepatic tissues, with relatively high levels in the stomach, pancreas, kidney and liver. Based on the kinetic parameters determined using recombinant human AKR7A3 and AFB1 dihydrodiol at pH 7.4, the catalytic efficiency of this reaction (k2/K, per M/s) equals or exceeds those reported for other enzymes, for example cytochrome P450s and glutathione S-transferases, known to metabolize AFB1 in vivo. These findings indicate that, depending on the extent of AFB1 dihydrodiol formation, AKR

  10. The Aldo-Keto Reductase AKR1B10 Is Up-Regulated in Keloid Epidermis, Implicating Retinoic Acid Pathway Dysregulation in the Pathogenesis of Keloid Disease.

    PubMed

    Jumper, Natalie; Hodgkinson, Tom; Arscott, Guyan; Har-Shai, Yaron; Paus, Ralf; Bayat, Ardeshir

    2016-07-01

    Keloid disease is a recurrent fibroproliferative cutaneous tumor of unknown pathogenesis for which clinical management remains unsatisfactory. To obtain new insights into hitherto underappreciated aspects of keloid pathobiology, we took a laser capture microdissection-based, whole-genome microarray analysis approach to identify distinct keloid disease-associated gene expression patterns within defined keloid regions. Identification of the aldo-keto reductase enzyme AKR1B10 as highly up-regulated in keloid epidermis suggested that an imbalance of retinoic acid metabolism is likely associated with keloid disease. Here, we show that AKR1B10 transfection into normal human keratinocytes reproduced the abnormal retinoic acid pathway expression pattern we had identified in keloid epidermis. Cotransfection of AKR1B10 with a luciferase reporter plasmid showed reduced retinoic acid response element activity, supporting the hypothesis of retinoic acid synthesis deficiency in keloid epidermis. Paracrine signals released by AKR1B10-overexpressing keratinocytes into conditioned medium resulted in up-regulation of transforming growth factor-β1, transforming growth factor-β2, and collagens I and III in both keloid and normal skin fibroblasts, mimicking the typical profibrotic keloid profile. Our study results suggest that insufficient retinoic acid synthesis by keloid epidermal keratinocytes may contribute to the pathogenesis of keloid disease. We refocus attention on the role of injured epithelium in keloid disease and identify AKR1B10 as a potential new target in future management of keloid disease. PMID:27025872

  11. An ethoxyquin-inducible aldehyde reductase from rat liver that metabolizes aflatoxin B1 defines a subfamily of aldo-keto reductases.

    PubMed Central

    Ellis, E M; Judah, D J; Neal, G E; Hayes, J D

    1993-01-01

    Protection of liver against the toxic and carcinogenic effects of aflatoxin B1 (AFB1) can be achieved through the induction of detoxification enzymes by chemoprotectors such as the phenolic antioxidant ethoxyquin. We have cloned and sequenced a cDNA encoding an aldehyde reductase (AFB1-AR), which is expressed in rat liver in response to dietary ethoxyquin. Expression of the cDNA in Escherichia coli and purification of the recombinant enzyme reveals that the protein exhibits aldehyde reductase activity and is capable of converting the protein-binding dialdehyde form of AFB1-dihydrodiol to the nonbinding dialcohol metabolite. We show that the mRNA encoding this enzyme is markedly elevated in the liver of rats fed an ethoxyquin-containing diet, correlating with acquisition of resistance to AFB1. AFB1-AR represents the only carcinogen-metabolizing aldehyde reductase identified to date that is induced by a chemoprotector. Alignment of the amino acid sequence of AFB1-AR with other known and putative aldehyde reductases shows that it defines a subfamily within the aldo-keto reductase superfamily. Images Fig. 2 Fig. 3 Fig. 4 PMID:8234296

  12. Aldo-keto reductase family 1 B10 protein detoxifies dietary and lipid-derived alpha, beta-unsaturated carbonyls at physiological levels

    SciTech Connect

    Zhong, Linlin; Liu, Ziwen; Yan, Ruilan; Johnson, Stephen; Zhao, Yupei; Fang, Xiubin; Cao, Deliang

    2009-09-18

    Alpha, beta-unsaturated carbonyls are highly reactive mutagens and carcinogens to which humans are exposed on a daily basis. This study demonstrates that aldo-keto reductase family 1 member B10 (AKR1B10) is a critical protein in detoxifying dietary and lipid-derived unsaturated carbonyls. Purified AKR1B10 recombinant protein efficiently catalyzed the reduction to less toxic alcohol forms of crotonaldehyde at 0.90 {mu}M, 4-hydroxynonenal (HNE) at 0.10 {mu}M, trans-2-hexanal at 0.10 {mu}M, and trans-2,4-hexadienal at 0.05 {mu}M, the concentrations at or lower than physiological exposures. Ectopically expressed AKR1B10 in 293T cells eliminated immediately HNE at 1 (subtoxic) or 5 {mu}M (toxic) by converting to 1,4-dihydroxynonene, protecting the cells from HNE toxicity. AKR1B10 protein also showed strong enzymatic activity toward glutathione-conjugated carbonyls. Taken together, our study results suggest that AKR1B10 specifically expressed in the intestine is physiologically important in protecting the host cell against dietary and lipid-derived cytotoxic carbonyls.

  13. A novel aldo-keto reductase from Jatropha curcas L. (JcAKR) plays a crucial role in the detoxification of methylglyoxal, a potent electrophile.

    PubMed

    Mudalkar, Shalini; Sreeharsha, Rachapudi Venkata; Reddy, Attipalli Ramachandra

    2016-05-20

    Abiotic stress leads to the generation of reactive oxygen species (ROS) which further results in the production of reactive carbonyls (RCs) including methylglyoxal (MG). MG, an α, β-dicarbonyl aldehyde, is highly toxic to plants and the mechanism behind its detoxification is not well understood. Aldo-keto reductases (AKRs) play a role in detoxification of reactive aldehydes and ketones. In the present study, we cloned and characterised a putative AKR from Jatropha curcas (JcAKR). Phylogenetically, it forms a small clade with AKRs of Glycine max and Rauwolfia serpentina. JcAKR was heterologously expressed in Escherichia coli BL-21(DE3) cells and the identity of the purified protein was confirmed through MALDI-TOF analysis. The recombinant protein had high enzyme activity and catalytic efficiency in assays containing MG as the substrate. Protein modelling and docking studies revealed MG was efficiently bound to JcAKR. Under progressive drought and salinity stress, the enzyme and transcript levels of JcAKR were higher in leaves compared to roots. Further, the bacterial and yeast cells expressing JcAKR showed more tolerance towards PEG (5%), NaCl (200mM) and MG (5mM) treatments compared to controls. In conclusion, our results project JcAKR as a possible and potential target in crop improvement for abiotic stress tolerance. PMID:26995646

  14. A comparative structural analysis reveals distinctive features of co-factor binding and substrate specificity in plant aldo-keto reductases.

    PubMed

    Giuseppe, Priscila Oliveira de; Santos, Marcelo Leite Dos; Sousa, Sylvia Morais de; Koch, Karen E; Yunes, José Andrés; Aparicio, Ricardo; Murakami, Mario Tyago

    2016-06-10

    Plant aldo-keto reductases of the AKR4C subfamily play key roles during stress and are attractive targets for developing stress-tolerant crops. However, these AKR4Cs show little to no activity with previously-envisioned sugar substrates. We hypothesized a structural basis for the distinctive cofactor binding and substrate specificity of these plant enzymes. To test this, we solved the crystal structure of a novel AKR4C subfamily member, the AKR4C7 from maize, in the apo form and in complex with NADP(+). The binary complex revealed an intermediate state of cofactor binding that preceded closure of Loop B, and also indicated that conformational changes upon substrate binding are required to induce a catalytically-favorable conformation of the active-site pocket. Comparative structural analyses of homologues (AKR1B1, AKR4C8 and AKR4C9) showed that evolutionary redesign of plant AKR4Cs weakened interactions that stabilize the closed conformation of Loop B. This in turn decreased cofactor affinity and altered configuration of the substrate-binding site. We propose that these structural modifications contribute to impairment of sugar reductase activity in favor of other substrates in the plant AKR4C subgroup, and that catalysis involves a three-step process relevant to other AKRs. PMID:27154221

  15. The Grid Enabled Mass Storage System (GEMSS): the Storage and Data management system used at the INFN Tier1 at CNAF.

    NASA Astrophysics Data System (ADS)

    Ricci, Pier Paolo; Bonacorsi, Daniele; Cavalli, Alessandro; Dell'Agnello, Luca; Gregori, Daniele; Prosperini, Andrea; Rinaldi, Lorenzo; Sapunenko, Vladimir; Vagnoni, Vincenzo

    2012-12-01

    The storage system currently used in production at the INFN Tier1 at CNAF is the result of several years of case studies, software development and tests. This solution, called the Grid Enabled Mass Storage System (GEMSS), is based on a custom integration between a fast and reliable parallel filesystem (the IBM General Parallel File System, GPFS), with a complete integrated tape backend based on the Tivoli Storage Manager (TSM), which provides Hierarchical Storage Management (HSM) capabilities, and the Grid Storage Resource Manager (StoRM), providing access to grid users through a standard SRM interface. Since the start of the Large Hadron Collider (LHC) operation, all LHC experiments have been using GEMSS at CNAF for both disk data access and long-term archival on tape media. Moreover, during last year, GEMSS has become the standard solution for all other experiments hosted at CNAF, allowing the definitive consolidation of the data storage layer. Our choice has proved to be very successful during the last two years of production with continuous enhancements, accurate monitoring and effective customizations according to the end-user requests. In this paper a description of the system is reported, addressing recent developments and giving an overview of the administration and monitoring tools. We also discuss the solutions adopted in order to grant the maximum availability of the service and the latest optimization features within the data access process. Finally, we summarize the main results obtained during these last years of activity from the perspective of some of the end-users, showing the reliability and the high performances that can be achieved using GEMSS.

  16. The role of nuclear reactions in the problem of 0νββ decay and the NUMEN project at INFN-LNS

    NASA Astrophysics Data System (ADS)

    Cappuzzello, F.; Agodi, C.; Bondì, M.; Carbone, D.; Cavallaro, M.; Foti, A.

    2015-07-01

    An innovative technique to access the nuclear matrix elements entering the expression of the life time of the double beta decay by relevant cross sections of double charge exchange reactions is proposed. The basic point is the coincidence of the initial and final state wave-functions in the two classes of processes and the similarity of the transition operators, which in both cases present a superposition of Fermi, Gamow-Teller and rank-two tensor components with a relevant implicit momentum transfer. First pioneering experimental results obtained at the INFN-LNS laboratory for the 40Ca(18O,18Ne)40Ar reaction at 270 MeV, give encouraging indication on the capability of the proposed technique to access relevant quantitative information. A key aspect of the project is the use of the K800 Superconducting Cyclotron (CS) for the acceleration of the required high resolution and low emittance heavy- ion beams and of the MAGNEX large acceptance magnetic spectrometer for the detection of the ejectiles. The use of the high-order trajectory reconstruction technique, implemented in MAGNEX, allows to reach the high mass, angular and energy resolution required even at very low cross section. The LNS set-up is today an ideal one for this research even in a worldwide perspective. However a main limitation on the beam current delivered by the accelerator and the maximum rate accepted by the MAGNEX focal plane detector must be sensibly overcome in order to systematically provide accurate numbers to the neutrino physics community in all the studied cases. The upgrade of the LNS facilities in this view is part of this project.

  17. Characterization of hamster NAD+-dependent 3(17)β-hydroxysteroid dehydrogenase belonging to the aldo-keto reductase 1C subfamily.

    PubMed

    Endo, Satoshi; Noda, Misato; Ikari, Akira; Tatematsu, Kenjiro; El-Kabbani, Ossama; Hara, Akira; Kitade, Yukio; Matsunaga, Toshiyuki

    2015-11-01

    The cDNAs for morphine 6-dehydrogenase (AKR1C34) and its homologous aldo-keto reductase (AKR1C35) were cloned from golden hamster liver, and their enzymatic properties and tissue distribution were compared. AKR1C34 and AKR1C35 similarly oxidized various xenobiotic alicyclic alcohols using NAD(+), but differed in their substrate specificity for hydroxysteroids and inhibitor sensitivity. While AKR1C34 showed 3α/17β/20α-hydroxysteroid dehydrogenase activities, AKR1C35 efficiently oxidized various 3β- and 17β-hydroxysteroids, including biologically active 3β-hydroxy-5α/β-dihydro-C19/C21-steroids, dehydroepiandrosterone and 17β-estradiol. AKR1C35 also differed from AKR1C34 in its high sensitivity to flavonoids, which inhibited competitively with respect to 17β-estradiol (Ki 0.11-0.69 μM). The mRNA for AKR1C35 was expressed liver-specific in male hamsters and ubiquitously in female hamsters, whereas the expression of the mRNA for AKR1C34 displayed opposite sexual dimorphism. Because AKR1C35 is the first 317Β-HYDROXYSTEROID DEHYDROGENASE IN THE AKR SUPERFAMILY: , we also investigated the molecular determinants for the 3β-hydroxysteroid dehydrogenase activity by replacement of Val54 and Cys310 in AKR1C35 with the corresponding residues in AKR1C34, Ala and Phe, respectively. The mutation of Val54Ala, but not Cys310Phe, significantly impaired this activity, suggesting that Val54 plays a critical role in recognition of the steroidal substrate. PMID:26002966

  18. Aldo-keto Reductase 1C3 (AKR1C3) is overexpressed in skin squamous cell carcinoma (SCC) and affects SCC growth via prostaglandin metabolism

    PubMed Central

    Mantel, Alon; Carpenter-Mendini, Amanda; VanBuskirk, JoAnne; Pentland, Alice P.

    2014-01-01

    Aldo-keto reductase 1C3 (AKR1C3) is an enzyme involved in metabolizing prostaglandins (PGs) and sex hormones. It metabolizes PGD2 to 9α11β-PGF2, diverting the spontaneous conversion of PGD2 to the PPARγ agonist, 15-Deoxy-Delta-12,14-prostaglandin J2 (15d-PGJ2). AKR1C3 is overexpressed in various malignancies, suggesting a tumor promoting function. This work investigates AKR1C3 expression in human non-melanoma skin cancers, revealing overexpression in squamous cell carcinoma (SCC). Effects of AKR1C3 overexpression were then evaluated using 3 SCC cell lines. AKR1C3 was detected in all SCC cell lines and its expression was upregulated in response to its substrate, PGD2. Although attenuating AKR1C3 expression in SCC cells by siRNA did not affect growth, treatment with PGD2 and its dehydration metabolite, 15d-PGJ2, decreased SCC proliferation in a PPARγ-dependent manner. In addition, treatment with the PPARγ agonist pioglitazone profoundly inhibited SCC proliferation. Finally, we generated an SCC cell line that stably overexpressed AKR1C3 (SCC-AKR1C3). SCC-AKR1C3 metabolized PGD2 to 9α11β-PGF2 12 fold faster than the parent cell line and was protected from the anti-proliferative effect mediated by PGD2. This work suggests that PGD2 and its metabolite 15d-PGJ2 attenuate SCC proliferation in a PPARγ-dependent manner, therefore activation of PPARγ by agonists such as Pioglitazone may benefit those at high risk of SCC. PMID:24917395

  19. Fluoxetine elevates allopregnanolone in female rat brain but inhibits a steroid microsomal dehydrogenase rather than activating an aldo-keto reductase

    PubMed Central

    Fry, J P; Li, K Y; Devall, A J; Cockcroft, S; Honour, J W; Lovick, T A

    2014-01-01

    Background and Purpose Fluoxetine, a selective serotonin reuptake inhibitor, elevates brain concentrations of the neuroactive progesterone metabolite allopregnanolone, an effect suggested to underlie its use in the treatment of premenstrual dysphoria. One report showed fluoxetine to activate the aldo-keto reductase (AKR) component of 3α-hydroxysteroid dehydrogenase (3α-HSD), which catalyses production of allopregnanolone from 5α-dihydroprogesterone. However, this action was not observed by others. The present study sought to clarify the site of action for fluoxetine in elevating brain allopregnanolone. Experimental Approach Adult male rats and female rats in dioestrus were treated with fluoxetine and their brains assayed for allopregnanolone and its precursors, progesterone and 5α-dihydroprogesterone. Subcellular fractions of rat brain were also used to investigate the actions of fluoxetine on 3α-HSD activity in both the reductive direction, producing allopregnanolone from 5α-dihydroprogesterone, and the reverse oxidative direction. Fluoxetine was also tested on these recombinant enzyme activities expressed in HEK cells. Key Results Short-term treatment with fluoxetine increased brain allopregnanolone concentrations in female, but not male, rats. Enzyme assays on native rat brain fractions and on activities expressed in HEK cells showed fluoxetine did not affect the AKR producing allopregnanolone from 5α-dihydroprogesterone but did inhibit the microsomal dehydrogenase oxidizing allopregnanolone to 5α-dihydroprogesterone. Conclusions and Implications Fluoxetine elevated allopregnanolone in female rat brain by inhibiting its oxidation to 5α-dihydroprogesterone by a microsomal dehydrogenase. This is a novel site of action for fluoxetine, with implications for the development of new agents and/or dosing regimens to raise brain allopregnanolone. PMID:25161074

  20. Plant aldo-keto reductases (AKRs) as multi-tasking soldiers involved in diverse plant metabolic processes and stress defense: A structure-function update.

    PubMed

    Sengupta, Debashree; Naik, Dhiraj; Reddy, Attipalli R

    2015-05-01

    The aldo-keto reductase (AKR) superfamily comprises of a large number of primarily monomeric protein members, which reduce a broad spectrum of substrates ranging from simple sugars to potentially toxic aldehydes. Plant AKRs can be broadly categorized into four important functional groups, which highlight their roles in diverse plant metabolic reactions including reactive aldehyde detoxification, biosynthesis of osmolytes, secondary metabolism and membrane transport. Further, multiple overlapping functional aspects of plant AKRs including biotic and abiotic stress defense, production of commercially important secondary metabolites, iron acquisition from soil, plant-microbe interactions etc. are discussed as subcategories within respective major groups. Owing to the broad substrate specificity and multiple stress tolerance of the well-characterized AKR4C9 from Arabidopsis thaliana, protein sequences of all the homologues of AKR4C9 (A9-like proteins) from forty different plant species (Phytozome database) were analyzed. The analysis revealed that all A9-like proteins possess strictly conserved key catalytic residues (D-47, Y-52 and K-81) and belong to the pfam00248 and cl00470 AKR superfamilies. Based on structural homology of the three flexible loops of AKR4C9 (Loop A, B and C) responsible for broad substrate specificity, A9-like proteins found in Brassica rapa, Phaseolus vulgaris, Cucumis sativus, Populus trichocarpa and Solanum lycopersicum were predicted to have a similar range of substrate specificity. Thus, plant AKRs can be considered as potential breeding targets for developing stress tolerant varieties in the future. The present review provides a consolidated update on the current research status of plant AKRs with an emphasis on important functional aspects as well as their potential future prospects and an insight into the overall structure-function relationships of A9-like proteins. PMID:25840343

  1. Regulation of Aldo-keto-reductase family 1 B10 by 14-3-3ε and their prognostic impact of hepatocellular carcinoma

    PubMed Central

    Lu, Yi-Jhu; Liang, Shu-Man; Liu, Chia-Chia; Chen, Shyh-Chang; Wang, John; Shyue, Song-Kun; Liou, Jun-Yang

    2015-01-01

    14-3-3ε is overexpressed in hepatocellular carcinoma (HCC) and its expression significantly associates with a poor prognostic outcome. To uncover how 14-3-3ε contributes to the tumor progression of HCC, we investigated the potential downstream targets regulated by 14-3-3ε. We found that 14-3-3ε increases expression and nuclear translocation of β-catenin and that 14-3-3ε-induced cell proliferation is attenuated by β-catenin silencing in HCC cells. Moreover, 14-3-3ε induces aldo-keto reductase family 1 member B10 (AKR1B10) expression through the activation of β-catenin signaling. Knockdown of AKR1B10 by siRNAs abolished 14-3-3ε-induced in vitro cell proliferation, anchorage-independent growth as well as in vivo tumor growth. Furthermore, AKR1B10 silencing increased retinoic acid (RA) levels in the serum of tumor-bearing mice and RA treatment attenuated 14-3-3ε-induced HCC cell proliferation. We further examined 14-3-3ε and AKR1B10 expression and clinicopathological characteristics of HCC tumors. Although the expression of AKR1B10 was significantly correlated with 14-3-3ε, an increase of AKR1B10 expression in 14-3-3ε positive patients paradoxically had better overall survival and disease-free survival rates as well as lower metastatic incidence than those without an AKR1B10 increase. Finally, we found a loss of AKR1B10 expression in cells exhibiting a high capacity of invasiveness. Silencing of AKR1B10 resulted in inducing snail and vimentin expression in HCC cells. These results indicate that AKR1B10 may play a dual role during HCC tumor progression. Our results also indicate that 14-3-3ε regulates AKR1B10 expression by activating β-catenin signaling. A combination of 14-3-3ε with AKR1B10 is a potential therapeutic target and novel prognostic biomarker of HCC. PMID:26516929

  2. The role of aldo-keto reductase 1C3 (AKR1C3)-mediated prostaglandin D2 (PGD2) metabolism in keloids.

    PubMed

    Mantel, Alon; Newsome, Austin; Thekkudan, Theresa; Frazier, Robert; Katdare, Meena

    2016-01-01

    Keloids are progressively expanding scars, mostly prevalent in individuals of African descent. Previous data identified increased mast cell number and activation state in keloids suggesting a role in disease progression. The major eicosanoid secreted by mast cells is prostaglandin D2 (PGD2), a relatively unstable pro-inflammatory mediator which can be spontaneously converted to 15-deoxy-(Delta12,14)-prostaglandin J2(15d-PGJ2) or enzymatically metabolized to 9α,11β-PGF2 by aldo-keto reductase 1C3 (AKR1C3). In this work, we investigated the possible role of PGD2 and its metabolites in keloids using CRL1762 keloid fibroblasts (KF) and immunohistochemical staining. Our data suggested approximately 3-fold increase of tryptase-positive mast cell count in keloids compared with normal skin. Furthermore, AKR1C3 was overexpressed in the fibrotic area of keloids while relatively weak staining detected in normal skin. Metabolism of PGD2 to 9α,11β-PGF2 by both, KF and normal fibroblasts, was dependent on AKR1C3 as this reaction was attenuated in the presence of the AKR1C3 inhibitor, 2'-hydroxyflavanone, or in cells with decreased AKR1C3 expression. 15d-PGJ2, but not the other tested PGs, inhibited KF proliferation, attenuated KF-mediated collagen gel contraction and increased caspase-3 activation. In addition, treatment with 15d-PGJ2 activated P38-MAPK, induced reactive oxygen species and upregulated superoxide dismutase-1 (SOD-1). Finally, inhibition of P38-MAPK further augmented 15d-PGJ2-induced caspase-3 cleavage and attenuated its effect on SOD-1 transcription. This work suggests that localized dual inhibition of AKR1C3 and P38-MAPK may inhibit keloid progression. Inhibiting AKR1C3 activity may generate oxidative environment due to redirection of PGD2 metabolism towards 15d-PGJ2 while inhibition of P38-MAPK will sensitize keloid cells to ROS-induced apoptosis. PMID:26308156

  3. Genetic variation in aldo-keto reductase 1D1 (AKR1D1) affects the expression and activity of multiple cytochrome P450s.

    PubMed

    Chaudhry, Amarjit S; Thirumaran, Ranjit K; Yasuda, Kazuto; Yang, Xia; Fan, Yiping; Strom, Stephen C; Schuetz, Erin G

    2013-08-01

    Human liver gene regulatory (Bayesian) network analysis was previously used to identify a cytochrome P450 (P450) gene subnetwork with Aldo-keto reductase 1D1 (AKR1D1) as a key regulatory driver of this subnetwork. This study assessed the biologic importance of AKR1D1 [a key enzyme in the synthesis of bile acids, ligand activators of farnesoid X receptor (FXR), pregnane X receptor (PXR), and constitutive androstane receptor (CAR), known transcriptional regulators of P450s] to hepatic P450 expression. Overexpression of AKR1D1 in primary human hepatocytes led to increased expression of CYP3A4, CYP2C8, CYP2C9, CYP2C19, and CYP2B6. Conversely, AKR1D1 knockdown decreased expression of these P450s. We resequenced AKR1D1 from 98 donor livers and identified a 3'-untranslated region (UTR) (rs1872930) single nucleotide polymorphism (SNP) significantly associated with higher AKR1D1 mRNA expression. AKR1D1 3'-UTR-luciferase reporter studies showed that the variant allele resulted in higher luciferase activity, suggesting that the SNP increases AKR1D1 mRNA stability and/or translation efficiency. Consistent with AKR1D1's putative role as a driver of the P450 subnetwork, the AKR1D1 3'-UTR SNP was significantly associated with increased hepatic mRNA expression of multiple P450s (CYP3A4, CYP2C8, CYP2C9, CYP2C19, and CYP2B6) and CYP3A4, CYP2C8, CYP2C19, and CYP2B6 activities. After adjusting for multiple testing, the association remained significant for AKR1D1, CYP2C9, and CYP2C8 mRNA expression and CYP2C8 activity. These results provide new insights into the variation in expression and activity of P450s that can account for interindividual differences in drug metabolism/efficacy and adverse drug events. In conclusion, we provide the first experimental evidence supporting a role for AKR1D1 as a key genetic regulator of the P450 network. PMID:23704699

  4. Exposure to 9,10-phenanthrenequinone accelerates malignant progression of lung cancer cells through up-regulation of aldo-keto reductase 1B10

    SciTech Connect

    Matsunaga, Toshiyuki; Morikawa, Yoshifumi; Haga, Mariko; Endo, Satoshi; Soda, Midori; Yamamura, Keiko; El-Kabbani, Ossama; Tajima, Kazuo; Ikari, Akira; Hara, Akira

    2014-07-15

    Inhalation of 9,10-phenanthrenequinone (9,10-PQ), a major quinone in diesel exhaust, exerts fatal damage against a variety of cells involved in respiratory function. Here, we show that treatment with high concentrations of 9,10-PQ evokes apoptosis of lung cancer A549 cells through production of reactive oxygen species (ROS). In contrast, 9,10-PQ at its concentrations of 2 and 5 μM elevated the potentials for proliferation, invasion, metastasis and tumorigenesis, all of which were almost completely inhibited by addition of an antioxidant N-acetyl-L-cysteine, inferring a crucial role of ROS in the overgrowth and malignant progression of lung cancer cells. Comparison of mRNA expression levels of six aldo-keto reductases (AKRs) in the 9,10-PQ-treated cells advocated up-regulation of AKR1B10 as a major cause contributing to the lung cancer malignancy. In support of this, the elevation of invasive, metastatic and tumorigenic activities in the 9,10-PQ-treated cells was significantly abolished by the addition of a selective AKR1B10 inhibitor oleanolic acid. Intriguingly, zymographic and real-time PCR analyses revealed remarkable increases in secretion and expression, respectively, of matrix metalloproteinase 2 during the 9,10-PQ treatment, and suggested that the AKR1B10 up-regulation and resultant activation of mitogen-activated protein kinase cascade are predominant mechanisms underlying the metalloproteinase induction. In addition, HPLC analysis and cytochrome c reduction assay in in vitro 9,10-PQ reduction by AKR1B10 demonstrated that the enzyme catalyzes redox-cycling of this quinone, by which ROS are produced. Collectively, these results suggest that AKR1B10 is a key regulator involved in overgrowth and malignant progression of the lung cancer cells through ROS production due to 9,10-PQ redox-cycling. - Highlights: • 9,10-PQ promotes invasion, metastasis and tumorigenicity in lung cancer cells. • The 9,10-PQ-elicited promotion is possibly due to AKR1B10 up

  5. Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of aldo-keto reductase 7 family proteins.

    PubMed Central

    Kelly, Vincent P; Sherratt, Philip J; Crouch, Dorothy H; Hayes, John D

    2002-01-01

    The aldo-keto reductase (AKR) 7 family is composed of the dimeric aflatoxin B(1) aldehyde reductase (AFAR) isoenzymes. In the rat, two AFAR subunits exist, designated rAFAR1 and rAFAR2. Herein, we report the molecular cloning of rAFAR2, showing that it shares 76% sequence identity with rAFAR1. By contrast with rAFAR1, which comprises 327 amino acids, rAFAR2 contains 367 amino acids. The 40 extra residues in rAFAR2 are located at the N-terminus of the polypeptide as an Arg-rich domain that may form an amphipathic alpha-helical structure. Protein purification and Western blotting have shown that the two AFAR subunits are found in rat liver extracts as both homodimers and as a heterodimer. Reductase activity in rat liver towards 2-carboxybenzaldehyde (CBA) was resolved by anion-exchange chromatography into three peaks containing rAFAR1-1, rAFAR1-2 and rAFAR2-2 dimers. These isoenzymes are functionally distinct; with NADPH as cofactor, rAFAR1-1 has a low K(m) and high activity with CBA, whereas rAFAR2-2 exhibits a low K(m) and high activity towards succinic semialdehyde. These data suggest that rAFAR1-1 is a detoxication enzyme, while rAFAR2-2 serves to synthesize the endogenous neuromodulator gamma-hydroxybutyrate (GHB). Subcellular fractionation of liver extracts showed that rAFAR1-1 was recovered in the cytosol whereas rAFAR2-2 was associated with the Golgi apparatus. The distinct subcellular localization of the rAFAR1 and rAFAR2 subunits was confirmed by immunocytochemistry in H4IIE cells. Association of rAFAR2-2 with the Golgi apparatus presumably facilitates secretion of GHB, and the novel N-terminal domain may either determine the targeting of the enzyme to the Golgi or regulate the secretory process. A murine AKR protein of 367 residues has been identified in expressed sequence tag databases that shares 91% sequence identity with rAFAR2 and contains the Arg-rich extended N-terminus of 40 amino acids. Further bioinformatic evidence is presented that full

  6. Purification from rat liver of a novel constitutively expressed member of the aldo-keto reductase 7 family that is widely distributed in extrahepatic tissues.

    PubMed Central

    Kelly, V P; Ireland, L S; Ellis, E M; Hayes, J D

    2000-01-01

    Antiserum raised against human aflatoxin B(1) aldehyde reductase 1 (hAFAR1) has been used to identify a previously unrecognized rat aldo-keto reductase (AKR). This novel enzyme is designated rat aflatoxin B(1) aldehyde reductase 2 (rAFAR2) and it characteristically migrates faster during SDS/PAGE than does the archetypal ethoxyquin-inducible rAFAR protein (now called rAFAR1). Significantly, rAFAR2 is essentially unreactive with polyclonal antibodies raised against rAFAR1. Besides its distinct electrophoretic and immunochemical properties, rAFAR2 appears to be regulated differently from rAFAR1 as it is expressed in most rat tissues and does not appear to be induced by ethoxyquin. Multiple forms of rAFAR2 have been identified. Anion-exchange chromatography on Q-Sepharose, followed by adsorption chromatography on columns of Matrex Orange A and Cibacron Blue, have been employed to purify rAFAR2 from rat liver cytosol. The Q-Sepharose chromatography step resulted in the resolution of rAFAR2 into three peaks of AKR activity, two of which were purified and shown to be capable of catalysing the reduction of 2-carboxybenzaldehyde, succinic semialdehyde, 4-nitrobenzaldehyde and 9,10-phenathrenequinone. The two most highly purified rAFAR2-containing preparations eluted from the Cibacron Blue column were 91 and 98% homogeneous. Analysis of these by SDS/PAGE indicated that the least anionic (peak CBA5) comprised a polypeptide of 37.0 kDa, whereas the most anionic (peak CBA6) contained two closely migrating polypeptides of 36.8 and 37.0 kDa; by contrast, in the present study, rAFAR1 was estimated by SDS/PAGE to be composed of 38.0 kDa subunits. Final purification of the 37 kDa polypeptide in CBA5 and CBA6 was accomplished by reversed-phase HPLC. Partial proteolysis of the two preparations of the 37 kDa polypeptide with Staphylococcus aureus V8 protease yielded fragments of identical size, suggesting that they represent the product of a single gene. Furthermore, the peptide maps

  7. A Novel Aldo-Keto Reductase (AKR17A1) of Anabaena sp. PCC 7120 Degrades the Rice Field Herbicide Butachlor and Confers Tolerance to Abiotic Stresses in E. coli.

    PubMed

    Agrawal, Chhavi; Sen, Sonia; Yadav, Shivam; Rai, Shweta; Rai, Lal Chand

    2015-01-01

    Present study deals with the identification of a novel aldo/keto reductase, AKR17A1 from Anabaena sp. PCC7120 and adds on as 17th family of AKR superfamily drawn from a wide variety of organisms. AKR17A1 shares many characteristics of a typical AKR such as- (i) conferring tolerance to multiple stresses like heat, UV-B, and cadmium, (ii) excellent activity towards known AKR substrates (isatin and 2-nitrobenzaldehyde), and (iii) obligate dependence on NADPH as a cofactor for enzyme activity. The most novel attribute of AKR17A1, first reported in this study, is its capability to metabolize butachlor, a persistent rice field herbicide that adversely affects agro-ecosystem and non-target organisms. The AKR17A1 catalyzed- degradation of butachlor resulted into formation of 1,2-benzene dicarboxylic acid and 2,6 bis (1,1, dimethylethyl) 4,-methyl phenol as the major products confirmed by GC-MS analysis. PMID:26372161

  8. A Novel Aldo-Keto Reductase (AKR17A1) of Anabaena sp. PCC 7120 Degrades the Rice Field Herbicide Butachlor and Confers Tolerance to Abiotic Stresses in E. coli

    PubMed Central

    Agrawal, Chhavi; Sen, Sonia; Yadav, Shivam; Rai, Shweta; Rai, Lal Chand

    2015-01-01

    Present study deals with the identification of a novel aldo/keto reductase, AKR17A1 from Anabaena sp. PCC7120 and adds on as 17th family of AKR superfamily drawn from a wide variety of organisms. AKR17A1 shares many characteristics of a typical AKR such as— (i) conferring tolerance to multiple stresses like heat, UV-B, and cadmium, (ii) excellent activity towards known AKR substrates (isatin and 2-nitrobenzaldehyde), and (iii) obligate dependence on NADPH as a cofactor for enzyme activity. The most novel attribute of AKR17A1, first reported in this study, is its capability to metabolize butachlor, a persistent rice field herbicide that adversely affects agro-ecosystem and non-target organisms. The AKR17A1 catalyzed- degradation of butachlor resulted into formation of 1,2-benzene dicarboxylic acid and 2,6 bis (1,1, dimethylethyl) 4,-methyl phenol as the major products confirmed by GC-MS analysis. PMID:26372161

  9. Development of Potent and Selective Inhibitors of Aldo-Keto Reductase 1C3 (type 5 17β-Hydroxysteroid Dehydrogenase) Based on N-Phenyl-Aminobenzoates and Their Structure Activity Relationships

    PubMed Central

    Adeniji, Adegoke O.; Twenter, Barry M.; Byrns, Michael C.; Jin, Yi; Chen, Mo; Winkler, Jeffrey D.; Penning, Trevor M.

    2012-01-01

    Aldo-keto reductase 1C3 (AKR1C3; type 5 17β-hydroxysteroid dehydrogenase) is overexpressed in castrate resistant prostate cancer (CRPC) and is implicated in the intratumoral biosynthesis of testosterone and 5α-dihydrotestosterone. Selective AKR1C3 inhibitors are required since compounds should not inhibit the highly related AKR1C1 and AKR1C2 isoforms which are involved in the inactivation of 5α-dihydrotestosterone. NSAIDs, N-phenylanthranilates in particular are potent but non-selective AKR1C3 inhibitors. Using flufenamic acid, 2-{[3-(trifluoromethyl)phenyl]amino}benzoic acid as lead compound, five classes of structural analogs were synthesized and evaluated for AKR1C3 inhibitory potency and selectivity. Structure activity relationship (SAR) studies revealed that a meta-carboxylic acid group relative to the amine conferred pronounced AKR1C3 selectivity without loss of potency, while electron withdrawing groups on the phenylamino B-ring were optimal for AKR1C3 inhibition. Lead compounds did not inhibit COX-1 or COX-2 but blocked the AKR1C3 mediated production of testosterone in LNCaP-AKR1C3 cells. These compounds offer promising leads towards new therapeutics for CRPC. PMID:22263837

  10. Aldo-keto reductase 1B10 promotes development of cisplatin resistance in gastrointestinal cancer cells through down-regulating peroxisome proliferator-activated receptor-γ-dependent mechanism.

    PubMed

    Matsunaga, Toshiyuki; Suzuki, Ayaka; Kezuka, Chihiro; Okumura, Naoko; Iguchi, Kazuhiro; Inoue, Ikuo; Soda, Midori; Endo, Satoshi; El-Kabbani, Ossama; Hara, Akira; Ikari, Akira

    2016-08-25

    Cisplatin (cis-diamminedichloroplatinum, CDDP) is one of the most effective chemotherapeutic drugs that are used for treatment of patients with gastrointestinal cancer cells, but its continuous administration often evokes the development of chemoresistance. In this study, we investigated alterations in antioxidant molecules and functions using a newly established CDDP-resistant variant of gastric cancer MKN45 cells, and found that aldo-keto reductase 1B10 (AKR1B10) is significantly up-regulated with acquisition of the CDDP resistance. In the nonresistant MKN45 cells, the sensitivity to cytotoxic effect of CDDP was decreased and increased by overexpression and silencing of AKR1B10, respectively. In addition, the AKR1B10 overexpression markedly suppressed accumulation and cytotoxicity of 4-hydroxy-2-nonenal that is produced during lipid peroxidation by CDDP treatment, suggesting that the enzyme acts as a crucial factor for facilitation of the CDDP resistance through inhibiting induction of oxidative stress by the drug. Transient exposure to CDDP and induction of the CDDP resistance decreased expression of peroxisome proliferator-activated receptor-γ (PPARγ) in MKN45 and colon cancer LoVo cells. Additionally, overexpression of PPARγ in the cells elevated the sensitivity to the CDDP toxicity, which was further augmented by concomitant treatment with a PPARγ ligand rosiglitazone. Intriguingly, overexpression of AKR1B10 in the cells resulted in a decrease in PPARγ expression, which was recovered by addition of an AKR1B10 inhibitor oleanolic acid, inferring that PPARγ is a downstream target of AKR1B10-dependent mechanism underlying the CDDP resistance. Combined treatment with the AKR1B10 inhibitor and PPARγ ligand elevated the CDDP sensitivity, which was almost the same level as that in the parental cells. These results suggest that combined treatment with the AKR1B10 inhibitor and PPARγ ligand is an effective adjuvant therapy for overcoming CDDP resistance of

  11. Molecular cloning, expression and catalytic activity of a human AKR7 member of the aldo-keto reductase superfamily: evidence that the major 2-carboxybenzaldehyde reductase from human liver is a homologue of rat aflatoxin B1-aldehyde reductase.

    PubMed Central

    Ireland, L S; Harrison, D J; Neal, G E; Hayes, J D

    1998-01-01

    The masking of charged amino or carboxy groups by N-phthalidylation and O-phthalidylation has been used to improve the absorption of many drugs, including ampicillin and 5-fluorouracil. Following absorption of such prodrugs, the phthalidyl group is hydrolysed to release 2-carboxybenzaldehyde (2-CBA) and the pharmaceutically active compound; in humans, 2-CBA is further metabolized to 2-hydroxymethylbenzoic acid by reduction of the aldehyde group. In the present work, the enzyme responsible for the reduction of 2-CBA in humans is identified as a homologue of rat aflatoxin B1-aldehyde reductase (rAFAR). This novel human aldo-keto reductase (AKR) has been cloned from a liver cDNA library, and together with the rat protein, establishes the AKR7 family of the AKR superfamily. Unlike its rat homologue, human AFAR (hAFAR) appears to be constitutively expressed in human liver, and is widely expressed in extrahepatic tissues. The deduced human and rat protein sequences share 78% identity and 87% similarity. Although the two AKR7 proteins are predicted to possess distinct secondary structural features which distinguish them from the prototypic AKR1 family of AKRs, the catalytic- and NADPH-binding residues appear to be conserved in both families. Certain of the predicted structural features of the AKR7 family members are shared with the AKR6 beta-subunits of voltage-gated K+-channels. In addition to reducing the dialdehydic form of aflatoxin B1-8,9-dihydrodiol, hAFAR shows high affinity for the gamma-aminobutyric acid metabolite succinic semialdehyde (SSA) which is structurally related to 2-CBA, suggesting that hAFAR could function as both a SSA reductase and a 2-CBA reductase in vivo. This hypothesis is supported in part by the finding that the major peak of 2-CBA reductase activity in human liver co-purifies with hAFAR protein. PMID:9576847

  12. The CCAAT-box binding transcription factor, Nuclear Factor-Y (NF-Y) regulates transcription of human aldo-keto reductase 1C1 (AKR1C1) gene

    PubMed Central

    Pallai, Rajash; Simpkins, Henry; Chen, Jianli; Parekh, Hemant K.

    2010-01-01

    Dihydrodiol dehydrogenases are a family of aldo-keto reductases (AKR1Cs) involved in the metabolism of steroid hormones and xenobiotics. Herein, we have cloned and characterized the proximal promoter region of the human AKR1C1 gene. The 5’ flanking proximal promoter region of the AKR1C1 gene consists of a TATA box and an inverted CCAAT binding site. Deletion analysis of the 5’-flanking, ~3.0 kb region of the human AKR1C1 gene identified the region between −128 to −88 as the minimal proximal promoter essential for basal transcription of AKR1C1 in human ovarian (2008 & 2008/C13*), lung (H23 & A549) and liver carcinoma (HepG2) cells. Site-directed mutagenesis studies indicated that the transcription factor binding sites for NF-Y/CEBP were involved in controlling the basal transcription of AKR1C1 in all the cancer cells studied. Electrophoretic mobility shift (EMSAs) and gel supershift assays demonstrated that the transcription factor NF-Y preferentially binds to the inverted CCAAT box at −109ATTGG−105 of the AKR1C1 gene. Chromatin immunoprecipitation (ChIP) analysis confirmed the in vivo association between NF-Y and human AKR1C1 gene promoter in human ovarian, lung and liver carcinoma cells. Ectopic expression of NF-Y’s increased the AKR1C1 gene transcription, whereas expression of a dominant-negative NF-YA or suppression of NF-YA decreased the AKR1C1 gene transcription. A 2-fold increase in AKR1C1 transcription was observed specifically in cisplatin-treated 2008 cells that was CCAAT box-dependent. These results indicate that the NF-Y regulates the basal transcription of AKR1C1 in human ovarian, lung and liver carcinoma cells and the cisplatin-induced transcription in human ovarian carcinoma cells. PMID:20338228

  13. A Novel Aldo-Keto Reductase, HdRed, from the Pacific Abalone Haliotis discus hannai, Which Reduces Alginate-derived 4-Deoxy-L-erythro-5-hexoseulose Uronic Acid to 2-Keto-3-deoxy-D-gluconate.

    PubMed

    Mochizuki, Shogo; Nishiyama, Ryuji; Inoue, Akira; Ojima, Takao

    2015-12-25

    Abalone feeds on brown seaweeds and digests seaweeds' alginate with alginate lyases (EC 4.2.2.3). However, it has been unclear whether the end product of alginate lyases (i.e. unsaturated monouronate-derived 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH)) is assimilated by abalone itself, because DEH cannot be metabolized via the Embden-Meyerhof pathway of animals. Under these circumstances, we recently noticed the occurrence of an NADPH-dependent reductase, which reduced DEH to 2-keto-3-deoxy-D-gluconate, in hepatopancreas extract of the pacific abalone Haliotis discus hannai. In the present study, we characterized this enzyme to some extent. The DEH reductase, named HdRed in the present study, could be purified from the acetone-dried powder of hepatopancreas by ammonium sulfate fractionation followed by conventional column chromatographies. HdRed showed a single band of ∼ 40 kDa on SDS-PAGE and reduced DEH to 2-keto-3-deoxy-D-gluconate with an optimal temperature and pH at around 50 °C and 7.0, respectively. HdRed exhibited no appreciable activity toward 28 authentic compounds, including aldehyde, aldose, ketose, α-keto-acid, uronic acid, deoxy sugar, sugar alcohol, carboxylic acid, ketone, and ester. The amino acid sequence of 371 residues of HdRed deduced from the cDNA showed 18-60% identities to those of aldo-keto reductase (AKR) superfamily enzymes, such as human aldose reductase, halophilic bacterium reductase, and sea hare norsolorinic acid (a polyketide derivative) reductase-like protein. Catalytic residues and cofactor binding residues known in AKR superfamily enzymes were fairly well conserved in HdRed. Phylogenetic analysis for HdRed and AKR superfamily enzymes indicated that HdRed is an AKR belonging to a novel family. PMID:26555267

  14. Protective roles of aldo-keto reductase 1B10 and autophagy against toxicity induced by p-quinone metabolites of tert-butylhydroquinone in lung cancer A549 cells.

    PubMed

    Endo, Satoshi; Nishiyama, Ayako; Suyama, Miho; Takemura, Mayuko; Soda, Midori; Chen, Huayue; Tajima, Kazuo; El-Kabbani, Ossama; Bunai, Yasuo; Hara, Akira; Matsunaga, Toshiyuki; Ikari, Akira

    2015-06-01

    tert-Butylhydroquinone (BHQ), an antioxidant used as a food additive, exhibits an anticancer effect at low doses, but is carcinogenic in rodents at high doses. BHQ is metabolized into cytotoxic tert-butylquinone (TBQ), which is further converted to 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one (TBEH) through 6-tert-butyl-2,3-epoxy-4-benzoquinone (TBE). Both TBQ and TBE are cytotoxic, but their toxic mechanisms have not been fully characterized. In this study, we have investigated the toxic mechanisms of TBQ and TBE, and the defense system against the two p-quinones using lung cancer A549 cells. TBQ and TBE, but not BHQ and TBEH, showed cytotoxicity to A549 cells. Neither caspase-3 activation nor an increase in the expression of endoplasmic reticulum stress-associating target genes was observed. TBQ and TBE reacted with reduced glutathione, and significantly decreased the glutathione level in A549 cells, suggesting that the cytotoxicity of the p-quinones is caused by their high electrophilicity reacting with biomolecules. The A549 cells treated with the p-quinones also showed increased levels of autophagic vacuoles and LC3-II protein, which are specific autophagy markers. An autophagy inhibitor, 3-methyladenine (3MA), decreased the LC3-II production by the p-quinones, but enhanced the cytotoxicity induced by TBQ and TBE, suggesting that autophagy contributes to alleviating the p-quinone-triggered cytotoxicity. In addition, the TBE-induced cytotoxicity and autophagy activation in the cells were significantly suppressed by overexpression of aldo-keto reductase (AKR)1B10 that efficiently reduces TBE into TBEH, and were augmented by pretreatment with a potent AKR1B10 inhibitor, C1. The effects of 3MA and C1 on the TBE-induced cytotoxicity were additive. The data provides evidence for the first time that autophagy and AKR1B10 contribute to the defense system against the cytotoxicity caused by the electrophilic p-quinone metabolites of BHQ. PMID:25289770

  15. Identification, cloning and regulation of cDNA encoding aldo-keto reductase 1B7 in the adrenal gland of two Saharan rodents Meriones libycus (Libyan jird) and Gerbillus gerbillus (gerbil).

    PubMed

    Mataoui-Mazari, Houria; Amirat, Zaïna; Khammar, Farida; Martinez, Antoine

    2011-12-01

    Aldo-Keto Reductase 1B7 (AKR1B7) is a mouse aldose reductase-like protein with two major sites of expression, the vas deferens and the adrenal cortex. In the adrenal cortex, Akr1b7 is an adrenocorticotropin (ACTH)-responsive-gene whose product scavenges harmful byproducts of steroidogenesis and limits stress response through the biosynthesis of prostaglandin F2α. The purpose of the present study was to explore the possible expression of AKR1B7 in the adrenal glands of two saharan rodents, Libyan jird and Lesser Egyptian gerbil. Western blot analyses demonstrated that a protein related to murine/rat AKR1B7 was highly expressed in adrenals and absent from vas deferens of both saharan species. Based on conserved sequences between mouse and rat, full length cDNA were cloned and sequenced in both species while hormonal regulation and tissue localization were explored in Libyan jird. Both cDNA encoded the expected 316 amino acids protein typical of AKR1B subfamily and contained the highly conserved catalytic tetrad consisting in Asp-44, Tyr-49, Lys-78 and His-111 residues. The deduced proteins shared higher identities with aldose reductase-like, i.e. AKR1B7 (86-94%), AKR1B8 and AKR1B10 (83-86%) than with aldose reductase group, i.e. AKR1B1 and AKR1B3 (70%). Phylogenetic analysis showed that the Libyan jird and gerbil enzymes were more closely related to murine and rat AKR1B7 than to the other AKR1B members. Northern blot analyses of total RNA from Libyan jird adrenals showed a single mRNA transcript of 1.4 kb whose expression was dependent on circulating ACTH levels. In conclusion, we demonstrate here that adrenal glands of Libyan jird and gerbil express both an ortholog of the murine/rat Akr1b7 gene and that ACTH-responsiveness is at least conserved in Libyan jird. PMID:21963864

  16. Specificity of Human Aldo-Keto Reductases, NAD(P)H: Quinone Oxidoreductase and Carbonyl Reductases to Redox-Cycle Polycyclic Aromatic Hydrocarbon Diones and 4-Hydroxyequilenin-o-Quinone

    PubMed Central

    Shultz, Carol A.; Quinn, Amy M.; Park, Jong-Heum; Harvey, Ronald G.; Bolton, Judy L; Maser, Edmund; Penning, Trevor M.

    2011-01-01

    Polycyclic aromatic hydrocarbons (PAH) are suspect human lung carcinogens and can be metabolically activated to remote quinones, e.g. benzo[a]pyrene-1,6-dione (B[a]P-1,6-dione) and B[a]P-3,6-dione by the action of either P450 monooxygenase or peroxidases and to non-K region o-quinones by aldo-keto reductases (AKRs). B[a]P-7,8-dione also structurally resembles 4-hydroxyequilenin o-quinone. These three classes of quinones can redox cycle, generate reactive oxygen species (ROS) and produce the mutagenic lesion 8-oxo-dGuo, and may contribute to PAH- and estrogen-induced carcinogenesis. We compared the ability of a complete panel of human recombinant AKRs to catalyze reduction of PAH o-quinones in the phenanthrene, chrysene, pyrene and anthracene series. The specific activities for NADPH-dependent quinone reduction were often 100-1,000 times greater than the ability of the same AKR isoform to oxidize the cognate PAH-trans-dihydrodiol. However, the AKR with the highest quinone reductase activity for a particular PAH o-quinone was not always identical to the AKR isoform with the highest dihydrodiol dehydrogenase activity for the respective PAH-trans-dihydrodiol. Discrete AKRs also catalyzed the reduction of B[a]P-1,6-dione, B[a]P-3,6-dione and 4-hydroxyequilenin o-quinone. Concurrent measurements of oxygen consumption, superoxide anion and hydrogen peroxide formation established that ROS were produced as a result of the redox-cycling. When compared with human recombinant NAD(P)H: quinone oxidoreductase (NQO1) and carbonyl reductases (CBR1 and CBR3), NQO1 was a superior catalyst of these reactions followed by AKRs and lastly CBR1 and CBR3. In A549 cells two-electron reduction of PAH o-quinones causes intracellular ROS formation. ROS formation was unaffected by the addition of dicumarol suggesting that NQO1 is not responsible for the two-electron reduction observed and does not offer protection against ROS formation from PAH o-quinones. PMID:21910479

  17. List of Participants

    NASA Astrophysics Data System (ADS)

    2014-07-01

    Alba Paolo (Università di Torino) Becattini Francesco (Università di Firenze) Bombaci Ignazio (Università di Pisa) Bonaccorso Angela (INFN Pisa) Colonna Maria (INFN-LNS Catania) Coraggio Luigi (INFN Napoli) Covello Aldo (Università di Napoli) Di Toro Massimo (Università di Catania) De Angelis Giacomo (INFN-LNL Legnaro) Gargano Angela (INFN Napoli) Gattobigio Mario (INLN, Université de Nice-Sophia Antipolis, CNRS, France) Gensini Paolo (INFN Lecce) Giannini Mauro (Università di Genova) Girlanda Luca (Università del Salento) Giusti Carlotta (Università di Pavia) Greco Vincenzo (Università di Catania) Grossi Eduardo (Università di Firenze) Itaco Nunzio (Università di Napoli) Kievsky Alejandro (INFN Pisa) Lanza Edoardo (INFN Catania) Lavagno Andrea (Politecnico di Torino) Logoteta Domenico (Universidade de Coimbra, Coimbra, Portugal) Lo Iudice Nicola (Università di Napoli) Lombardo Maria Paola (INFN-LNF Frascati) Lo Meo Sergio (ENEA Bologna) Mannarelli Massimo (INFN-LNGS Assergi) Marcucci Laura Elisa (Università di Pisa) Matera Francesco (Università di Firenze) Orlandini Giuseppina (Università di Trento) Pacati Franco (Università di Pavia) Pederiva Francesco (Università di Trento) Pirrone Sara (INFN Catania) Puglisi Armando (Università di Catania) Radici Marco (INFN Pavia) Rinaldi Matteo (Università di Perugia) Roggero Alessandro (Università di Trento) Rolando Valentina (Università di Ferrara) Rosati Sergio (Università di Pisa) Ruggieri Marco (Università di Catania) Salmè Gianni (INFN Roma) Santopinto Elena (INFN Genova) Scopetta Sergio (Università di Perugia) Taiuti Mauro (Università di Genova) Vigezzi Enrico (INFN Milano) Viviani Michele (INFN Pisa) Vorabbi Matteo (Università di Pavia)

  18. Fine tuning of coenzyme specificity in family 2 aldo-keto reductases revealed by crystal structures of the Lys-274 → Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD + and NADP +

    SciTech Connect

    Leitgeb, Stefan; Petschacher, Barbara; Wilson, David K.; Nidetzky, Bernd

    2005-01-11

    Aldo-keto reductases of family 2 employ single site replacement Lys → Arg to switch their cosubstrate preference from NADPH to NADH. X-ray crystal structures of Lys-274 → Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+ and NADP+ were determined at a resolution of 2.4 and 2.3 Å, respectively. Due to steric conflicts in the NADP+-bound form, the arginine side chain must rotate away from the position of the original lysine side chain, thereby disrupting a network of direct and water-mediated interactions between Glu-227, Lys-274 and the cofactor 2'-phosphate and 3'-hydroxy groups. Because anchoring contacts of its Glu-227 are lost, the coenzyme-enfolding loop that becomes ordered upon binding of NAD(P)+ in the wild-type remains partly disordered in the NADP+-bound mutant. The results delineate a catalytic reaction profile for the mutant in comparison to wild-type.

  19. Life as a Sober Citizen: Aldo Leopold's Wildlife Ecology 118

    ERIC Educational Resources Information Center

    Theiss, Nancy Stearns

    2009-01-01

    This historic case study addressed the issue of the lack of citizen action toward environmentally responsible behavior. Although there have been studies regarding components of environmental responsible behavior [ERB], there has been little focus on historic models of exemplary figures of ERB. This study examined one of the first conservation…

  20. Aldo-Keto Reductases 1B in Endocrinology and Metabolism

    PubMed Central

    Pastel, Emilie; Pointud, Jean-Christophe; Volat, Fanny; Martinez, Antoine; Lefrançois-Martinez, Anne-Marie

    2012-01-01

    The aldose reductase (AR; human AKR1B1/mouse Akr1b3) has been the focus of many research because of its role in diabetic complications. The starting point of these alterations is the massive entry of glucose in polyol pathway where it is converted into sorbitol by this enzyme. However, the issue of AR function in non-diabetic condition remains unresolved. AR-like enzymes (AKR1B10, Akr1b7, and Akr1b8) are highly related isoforms often co-expressed with bona fide AR, making functional analysis of one or the other isoform a challenging task. AKR1B/Akr1b members share at least 65% protein identity and the general ability to reduce many redundant substrates such as aldehydes provided from lipid peroxidation, steroids and their by-products, and xenobiotics in vitro. Based on these properties, AKR1B/Akr1b are generally considered as detoxifying enzymes. Considering that divergences should be more informative than similarities to help understanding their physiological functions, we chose to review specific hallmarks of each human/mouse isoforms by focusing on tissue distribution and specific mechanisms of gene regulation. Indeed, although the AR shows ubiquitous expression, AR-like proteins exhibit tissue-specific patterns of expression. We focused on three organs where certain isoforms are enriched, the adrenal gland, enterohepatic, and adipose tissues and tried to connect recent enzymatic and regulation data with endocrine and metabolic functions of these organs. We presented recent mouse models showing unsuspected physiological functions in the regulation of glucido-lipidic metabolism and adipose tissue homeostasis. Beyond the widely accepted idea that AKR1B/Akr1b are detoxification enzymes, these recent reports provide growing evidences that they are able to modify or generate signal molecules. This conceptually shifts this class of enzymes from unenviable status of scavenger to upper class of messengers. PMID:22876234

  1. List of Participants

    NASA Astrophysics Data System (ADS)

    Bombaci, I.; Covello, A.; Marcucci, L. E.; Rosati, S.

    2009-07-01

    Armani Paolo (Università di Trento) Benhar Omar (INFN Roma) Bombaci Ignazio (Università di Pisa) Bonanno Luca (Università di Ferrara) Catara Francesco (Università di Catania) Cò Giampaolo (Università di Lecce) Colonna Maria (Laboratori Nazionali del Sud, INFN Catania) Colonna Nicola (INFN Bari) Conti Francesco (Università di Pavia) Coraggio Luigi (INFN Napoli) Covello Aldo (Università di Napoli) Cristoforetti Marco (Technische Universität München, Germania) Cuofano Carmine (Università di Ferrara) Di Toro Massimo (Università di Catania) Drago Alessandro (Università di Ferrara) Faccioli Pietro (Università di Trento) Farina Nicola (INFN Roma) Finelli Paolo (Università di Bologna) Fiorentini Giovanni (Università di Ferrara) Fortunato Lorenzo (Università di Padova) Gambacurta Danilo (Università di Catania) Gandolfi Stefano (Università di Trento) Gargano Angela (INFN Napoli) Giannini Mauro (Università di Genova) Girlanda Luca (INFN Pisa) Giusti Carlotta (INFN Pavia) Illarionov Alexei (SISSA Trieste) Itaco Nunzio (Università di Napoli) Kievsky Alejandro (INFN Pisa) Lanza Edoardo (INFN Catania) Leidemann Winfried (Università di Trento) Lenzi Silvia (Università di Padova) Lipparini Enrico (Università di Trento) Lissia Marcello (Università di Cagliari) Lo Iudice Nicola (Università di Napoli) Maieron Chiara (Università di Lecce) Marcucci Laura Elisa (Università di Pisa) Matera Francesco (Università di Firenze) Millo Raffaele (Università di Trento) Orlandini Giuseppina (Università di Trento) Pacati Franco (Università di Pavia) Pastore Alessandro (Univeristy of Jyväskylä, Finlandia) Pederiva Francesco (Università di Trento) Pisent Gualtiero (Università di Padova) Prete Gianfranco (INFN Laboratori Nazionali di Legnaro) Quarati Piero (Politecnico di Torino) Rosati Sergio (Università di Pisa) Salmè Giovanni (INFN Roma) Santopinto Elena (INFN Genova) Traini Marco (Università di Trento) Vigezzi Enrico (INFN Milano) Vitturi Andrea (Universit

  2. List of Participants

    NASA Astrophysics Data System (ADS)

    2011-08-01

    Abigail Alvarez OlarteCINVESTAV Alba Leticia Carrillo MonteverdeDCI-UG Alberto CarramiñanaINAOE Aldo MorselliFERMI Alejandro CastillaDCI-UG Alejandro IbarraTechnical University of Munich Alma D Rojas PachecoFCFM-BUAP Alma Xochitl Gonzalez MoralesInstituto de Ciencias Nucleares, UNAM Andrew Walcott BeckwithAmerican Institute of Beam Energy Physics Ariadna Montiel ArenasDepartamento de Física, CINVESTAV Arnulfo ZepedaCinvestav Arturo Alvarez CruzInstituto de Fisica, UNAM Axel de la MacorraUNAM, IAC Azar MustafayevUniversity of Minnesota Benjamin JaramilloDCI-UG Vincent BertinCPPM-Marseille Carlos Alberto Vaquera-AraujoDCI-UG Carlos MuñozMadrid Autonoma U. & Madrid, IFT Carmine PagliaroneINFN, FNAL Carolina Lujan PeschardDCI-UG Christiane Frigerio MartinsUniversidade Federal do ABC-São Paulo Csaba BalazsMonash University David DelepineDCI-UG David G CerdenoUniversidad Autonoma de Madrid & Instituto de Fisica Teorica Debasish MajumdarSaha Institute of Nuclear Physics, Kolkata, India Dibyendu PanigrahiKandi Raj College, Kandi, Murshidabad, INDIA-742137 Dupret Alberto Santana BejaranoUniversidad de Sonora Departamento de Investigacion en Fisica Ernest MaRiverside U.C. Esteban Alejandro Reyes Pírez MontañezInstituto de Física, UNAM Federico Ortiz TrejoINSTITUTO DE ASTRONOMÍA - UNAM Francisco José de Anda NavarroUniversidad de Guadalajara González Alvarez Francisco JavierCINVESTAV-Depto. Física Gustavo Medina TancoICN-UNAM Hernando Efrain Caicedo OrtizInstituto Politecnico Nacional - IPN J D VergadosCERN & Ioannina U. James R BoyceJefferson Lab Jason SteffenFERMILAB Javier Montaño DomínguezDCI-UG Jeevan SolankiMandsaur Institue of Technology MP India Joe SatoSaitama University Jorge Luis Navarro EstradaUNAM-ICN and Universidad del Atlantico (B/quilla-Col.) Jose A R CembranosUniversity of Minnesota José DíazIFIC Jose Didino Garcia AguilarDepto. de Fisica. Cinvestav Keith OliveUniversity of Minnesota Konstantia BalasiUniversity of Ioannina, Greece Lilian Prado

  3. New precision era of experiments on strong interaction with strangeness at DAFNE/LNF-INFN

    NASA Astrophysics Data System (ADS)

    Ishiwatari, T.; Bazzi, M.; Beer, G.; Berucci, C.; Bombelli, L.; Bragadireanu, A. M.; Cargnelli, M.; Curceanu, C.; d'Uffizi, A.; Fiorini, C.; Ghio, F.; Guaraldo, C.; Hayano, R. S.; Iliescu, M.; Iwasaki, M.; Kienle, P.; Levi Sandri, P.; Longoni, A.; Marton, J.; Okada, S.; Pietreanu, D.; Ponta, T.; Quaglia, R.; Romero Vidal, A.; Sbardella, E.; Scordo, A.; Shi, H.; Sirghi, D. L.; Sirghi, F.; Tatsuno, H.; Tudorache, A.; Tudorache, V.; Vazquez Doce, O.; Wünschek, B.; Widmann, E.; Zmeskal, J.

    2014-03-01

    The strong-interaction shifts and widths of kaonic hydrogen, deuterium, 3He, and 4He were measured in the SIDDHARTA experiment. The most precise values of the shift and width of the kaonic hydrogen 1s state were determined to be ɛ1s = -283 ± 36(stat)±6(syst) eV and Γ1s = 541±89(stat)±22(syst) eV. The upper limit of the kaonic deuterium Kα yield was found to be ≤ 0.39%. In addition, the shifts and widths of the kaonic 3He and 4He 2p states were determined to be ɛ2p(3He) = -2 ± 2(stat) ± 4(syst) eV and Γ2p(3He) = 6 ± 6(stat) ± 7(syst) eV; ɛ2p(4He) = +5 ± 3(stat) ± 4(syst) eV and Γ2p(4He) = 14 ± 8(stat) ± 5(syst) eV. These values are important for the constraints of the low-energy bar KN interaction in theoretical approaches.

  4. Preliminary measurements on the new TOF system installed at the AMS beamline of INFN-LABEC

    NASA Astrophysics Data System (ADS)

    Palla, L.; Castelli, L.; Czelusniak, C.; Fedi, M. E.; Giuntini, L.; Liccioli, L.; Mandò, P. A.; Martini, M.; Mazzinghi, A.; Ruberto, C.; Schiavulli, L.; Sibilia, E.; Taccetti, F.

    2015-10-01

    A high resolution time of flight (TOF) system has been developed at LABEC, the 3 MV Tandem accelerator laboratory in Florence, in order to improve the sensitivity of AMS measurements on carbon samples with ultra-low concentration and also to measure other isotopes, such as 129I. The system can be employed to detect and identify residual interfering particles originated from the break-up of molecular isobars. The set-up has been specifically designed for low energy heavy ions: it consists of two identical time pick-off stations, each made up of a thin conductive foil and a Micro-Channel Plate (MCP) multiplier. The beamline is also equipped with a silicon detector, installed downstream the stop TOF station. In this paper the design of the new system and the implemented readout electronics are presented. The tests performed on the single time pick-off station are reported: they show that the maximum contribution to the timing resolution given by both the intrinsic MCP resolution and the electronics is ⩽500 ps (FWHM). For these tests, single particle pulsed beams of 2-5 MeV protons and 10 MeV 12C3+ ions, to simulate typical AMS conditions, were used. The preliminary TOF and TOF-E (TOF-energy) measurements performed with carbon beams after the installation of the new system on the AMS beam line are also discussed. These measurements were performed using the foil-MCP as the start stage and a silicon detector as the stop stage. The spectra acquired with carbon ions suggest the presence of a small residual background from neighboring masses reaching the end of the beamline with the same energy as the rare isotope.

  5. Progress on the accelerator based SPES-BNCT project at INFN Legnaro

    SciTech Connect

    Esposito, J.; Colautti, P.; Pisent, A.; Conte, V.; Moro, D.; De Nardo, L.; Agosteo, S.; Rosi, G.

    2007-02-12

    In the framework of an advanced Exotic Ion Beam facility, named SPES (Study and Production of Exotic Species), that will allow a frontier program both in nuclear and interdisciplinary physics, an intense thermal neutron beam facility, devoted to perform Boron Neutron Capture Therapy (BNCT) experimental treatments on skin melanoma tumor, is currently under construction based on the SPES proton driver. A vast radiobiological investigation in vitro and in vivo has started with the new 10B carriers developed. Special microdosimetric detectors have been constructed to properly measure all the BNCT dose components and their qualities. Both microdosimetric and radiobiological measurements are being performed at the new HYTHOR beam shaping assembly at the Enea-Casaccia TAPIRO reactor.

  6. Reaction studies with low-energy weakly-bound beams at INFN-LNS

    NASA Astrophysics Data System (ADS)

    Di Pietro, A.; Fernandez-Garcia, J. P.; Figuera, P.; Fisichella, M.; Lattuada, M.; Torresi, D.; Zadro, M.

    2016-05-01

    The reaction dynamics of collisions involving halo or weakly bound nuclei, at energies around the Coulomb barrier, can be strongly affected by the structure of such nuclei. Very strong entrance channel effects have been observed on various reaction pocess such as, elastic scattering, fusion and direct reactions when comparing collision induced by the 6He and 11Be halo nuclei with the ones induced by their cores 4He and 10Be. Collisions induced by the stable weakly bound nuclei 6Li, 7Li show also some peculiarities in comparison to the ones induced by well bound nuclei; coupling with the break-up channel is in fact very important in reproducing low energy data. In this contribution an overview of our present understanding of the discussed topic will be given along with the discussion of some new preliminary results.

  7. Dosimetric and clinical experience in eye proton treatment at INFN-LNS

    SciTech Connect

    Cirrone, G. A. P.; Cuttone, G.; Di Rosa, F.; Lojacono, P.; Mongelli, V.; Patti, I. V.; Pittera, S.; Russo, G.; Valastro, L. M.; Lo Nigro, S.; Ott, J.; Reibaldi, A.; Privitera, G.; Raffaele, L.; Salamone, V.; Spatola, C.; Sabini, M. G.

    2009-05-04

    After six years of activity 155 patients have been treated inside the CATANA (Centro di AdroTerapia ed Applicazioni Nucleari Avanzate) facility. CATANA is the first and unique proton therapy facility in which the 62 MeV proton beams, accelerated by a Superconducting Cyclotron, are used for the radio-therapeutic treatments of choroidal and iris melanomas. Inside CATANA new absolute and relative dosimetric techniques have been developed in order to achieve the best results in terms of treatment precision and dose release accuracy. The follow-up results for 42 patients demonstrated the efficacy of high energy protons in the radiotherapeutic field and encouraged us in our activity in the battle against cancer.

  8. Oxidation of PAH trans-Dihydrodiols by Human Aldo-Keto Reductase AKR1B10

    PubMed Central

    Quinn, Amy M.; Harvey, Ronald G.; Penning, Trevor M.

    2009-01-01

    AKR1B10 has been identified as a potential biomarker for human non-small cell lung carcinoma and as a tobacco exposure and response gene. AKR1B10 functions as an efficient retinal reductase in vitro, and may regulate retinoic acid homeostasis. However, the possibility that this enzyme is able to activate polycyclic aromatic hydrocarbon (PAH) trans-dihydrodiols to form reactive and redox-active o-quinones has not been investigated to date. AKR1B10 was found to oxidize a wide range of PAH trans-dihydrodiol substrates in vitro to yield PAH o-quinones. Reactions of AKR1B10 proceeded with improper stereochemistry, since it was specific for the minor (+)-benzo[a]pyrene-7S,8S-dihydrodiol diastereomer formed in vivo. However, AKR1B10 displayed reasonable activity in the oxidation of both the (−)-R,R and (+)-S,S stereoisomers of benzo[g]chrysene-11,12-dihydrodiol and oxidized the potentially relevant, albeit minor, (+)-benz[a]anthracene-3S,4S-dihydrodiol metabolite. We find that AKR1B10 is therefore likely to play a contributing role in the activation of PAH trans-dihydrodiols in human lung. AKR1B10 retinal reductase activity was confirmed in vitro and found to be 5- to 150-fold greater than the oxidation of PAH trans-dihydrodiols examined. AKR1B10 was highly expressed at the mRNA and protein levels in human lung adenocarcinoma A549 cells, and robust retinal reductase activity was measured in lysates of these cells. The much greater catalytic efficiency of retinal reduction compared to PAH trans-dihydrodiol metabolism suggests AKR1B10 may play a greater role in lung carcinogenesis through dysregulation of retinoic acid homeostasis than through oxidation of PAH trans-dihydrodiols. PMID:18788756

  9. The "I-Thou" Relationship, Place-Based Education, and Aldo Leopold

    ERIC Educational Resources Information Center

    Knapp, Clifford E.

    2005-01-01

    This article describes a new educational field labeled "place-based education" and relates it to experiential learning. This term has appeared in the educational literature over the last 10 years and illustrates a concern for providing participants with quality experiences in local settings. After defining and describing the term, one aspect of…

  10. Perspectives from the Aldo Leopold Wilderness Research Institute: Amphibians and wilderness

    USGS Publications Warehouse

    Corn, Paul Stephen

    2001-01-01

    The decline of amphibian species has emerged as a major global conservation issue in the last decade. Last year, the Department of the Interior (DOI) initiated a major national initiative to detect trends in amphibian populations and research the causes of declines. The program, conducted principally by the U.S. Geological Survey (USGS), emphasizes lands managed by DOI, but collaboration with the Forest Service is encouraged to increase the scope of inference about population trends. Although amphibians are not usually the first group of animals that comes to mind when one thinks of wilderness, conservation of amphibian populations is clearly a wilderness issue.

  11. PREFACE: XIV Conference on Theoretical Nuclear Physics in Italy

    NASA Astrophysics Data System (ADS)

    Bombaci, I.; Covello, A.; Marcucci, L. E.; Rosati, S.

    2014-07-01

    This volume contains the invited and contributed papers presented at the 14th Conference on Theoretical Nuclear Physics in Italy held in Cortona, Italy, from 29-31 October, 2013. The meeting was held at the Palazzone, an elegant Renaissance Villa, commissioned by the Cardinal Silvio Passerini (1469-1529), Bishop of Cortona, and presently owned by the Scuola Normale Superiore di Pisa. The aim of this biennial Conference is to bring together Italian theorists working in various fields of nuclear physics to discuss their latest results and confront their points of view in a lively and informal way. This offers the opportunity to stimulate new ideas and promote collaborations between different research groups. The Conference was attended by 46 participants, coming from 13 Italian Universities and 11 Laboratories and Sezioni of the Istituto Nazionale di Fisica Nucleare - INFN. The program of the conference, prepared by the Organizing Committee (Ignazio Bombaci, Aldo Covello, Laura Elisa Marcucci and Sergio Rosati) focused on the following main topics: Few-Nucleon Systems Nuclear Structure Nuclear Matter and Nuclear Dynamics Relativistic Heavy Ion Collisions and Quark-Gluon Plasma Nuclear Astrophysics Nuclear Physics with Electroweak Probes Structure of Hadrons and Hadronic Matter. In the last session of the Conference there were two invited review talks related to experimental activities of great current interest. Giacomo De Angelis from the Laboratori Nazionali di Legnaro spoke about the INFN SPES radioactive ion beam project. Sara Pirrone, INFN Sezione di Catania, gave a talk on the symmetry energy and isospin physics with the CHIMERA detector. Finally, Mauro Taiuti (Università di Genova), National Coordinator of the INFN-CSN3 (Nuclear Physics Experiments), reported on the present status and future challenges of experimental nuclear physics in Italy. We gratefully acknowledge the financial support of INFN who helped make the conference possible. I Bombaci, A Covello

  12. Recent results of the laser ion source facility at INFN-LNS and applications to nuclear and applied research

    SciTech Connect

    Gammino, S.; Celona, L.; Torrisi, L.; Giuffrida, L.; Cavallaro, S.; Miracoli, R.; Margarone, D.; Mascali, D.

    2010-02-15

    A pulsed neodymium-doped yttrium aluminum garnet laser ion source has been used as proton beams generator. The laser wavelength is 1064 nm, the pulse duration is 9 ns and the intensity reaches 10{sup 10} W/cm{sup 2}. Laser irradiates hydrogenated polymers targets located in a chamber at 10{sup -7} mbar. The ions are post-accelerated in a suitable chamber by 30 kV of voltage between the target, positively biased, and the following ground electrode. The extracted beams is characterized through a time-of-flight technique. Possible applications to the field of nuclear physics, such as nuclear excitation and de-excitations, nuclear reactions and nuclear fusion, will be presented and discussed.

  13. Crystal structure of perakine reductase, founding member of a novel aldo-keto reductase (AKR) subfamily that undergoes unique conformational changes during NADPH binding.

    PubMed

    Sun, Lianli; Chen, Yixin; Rajendran, Chitra; Mueller, Uwe; Panjikar, Santosh; Wang, Meitian; Mindnich, Rebekka; Rosenthal, Cindy; Penning, Trevor M; Stöckigt, Joachim

    2012-03-30

    Perakine reductase (PR) catalyzes the NADPH-dependent reduction of the aldehyde perakine to yield the alcohol raucaffrinoline in the biosynthetic pathway of ajmaline in Rauvolfia, a key step in indole alkaloid biosynthesis. Sequence alignment shows that PR is the founder of the new AKR13D subfamily and is designated AKR13D1. The x-ray structure of methylated His(6)-PR was solved to 2.31 Å. However, the active site of PR was blocked by the connected parts of the neighbor symmetric molecule in the crystal. To break the interactions and obtain the enzyme-ligand complexes, the A213W mutant was generated. The atomic structure of His(6)-PR-A213W complex with NADPH was determined at 1.77 Å. Overall, PR folds in an unusual α(8)/β(6) barrel that has not been observed in any other AKR protein to date. NADPH binds in an extended pocket, but the nicotinamide riboside moiety is disordered. Upon NADPH binding, dramatic conformational changes and movements were observed: two additional β-strands in the C terminus become ordered to form one α-helix, and a movement of up to 24 Å occurs. This conformational change creates a large space that allows the binding of substrates of variable size for PR and enhances the enzyme activity; as a result cooperative kinetics are observed as NADPH is varied. As the founding member of the new AKR13D subfamily, PR also provides a structural template and model of cofactor binding for the AKR13 family. PMID:22334702

  14. Proceedings of the Conference of the International Group for the Psychology of Mathematics Education (29th, Melbourne, Australia, July 10-15, 2005). Volume 3

    ERIC Educational Resources Information Center

    Chick, Helen L., Ed.; Vincent, Jill L., Ed.

    2005-01-01

    The third volume of the 29th annual conference of the International Group for the Psychology of Mathematics Education contains full research report papers. Papers include: (1) Students' Use of ICT Tools: Choices and Reasons (Anne Berit Fuglestad); (2) Interaction of Modalities in Cabri: A Case Study (Fulvia Furinghetti, Francesca Morselli, and…

  15. Human liver-type fatty acid-binding protein protects against tubulointerstitial injury in aldosterone-induced renal injury.

    PubMed

    Ichikawa, Daisuke; Kamijo-Ikemori, Atsuko; Sugaya, Takeshi; Shibagaki, Yugo; Yasuda, Takashi; Hoshino, Seiko; Katayama, Kimie; Igarashi-Migitaka, Junko; Hirata, Kazuaki; Kimura, Kenjiro

    2015-01-15

    To demonstrate the renoprotective function of human liver-type fatty acid-binding protein (hL-FABP) expressed in proximal tubules in aldosterone (Aldo)-induced renal injury, hL-FABP chromosomal transgenic (Tg) and wild-type (WT) mice received systemic Aldo infusions (Tg-Aldo and WT-Aldo, respectively) were given 1% NaCl water for 28 days. In this model, elevation of systolic blood pressure, monocyte chemoattractant protein-1 expression, macrophage infiltration in the interstitium, tubulointerstitial damage, and depositions of type I and III collagens were observed. Elevation of systolic blood pressure did not differ in WT-Aldo vs. Tg-Aldo animals, however, renal injury was suppressed in Tg-Aldo compared with WT-Aldo mice. Dihydroethidium fluorescence was used to evaluate reactive oxidative stress, which was suppressed in Tg-Aldo compared with WT-Aldo mice. Gene expression of angiotensinogen in the kidney was upregulated, and excretion of urinary angiotensinogen was increased in WT-Aldo mice. This exacerbation was suppressed in Tg-Aldo mice. Expression of hL-FABP was upregulated in proximal tubules of Tg-Aldo mice. Urinary excretion of hL-FABP was significantly greater in Tg-Aldo than in Tg-control mice. In conclusion, hL-FABP ameliorated the tubulointerstitial damage in Aldo-induced renal injury via reducing oxidative stress and suppressing activation of the intrarenal renin-angiotensin system. PMID:25339700

  16. Ceramide Production Mediates Aldosterone-Induced Human Umbilical Vein Endothelial Cell (HUVEC) Damages

    PubMed Central

    Zhang, Yumei; Pan, Yu; Bian, Zhixiang; Chen, Peihua; Zhu, Shijian; Gu, Huiyi; Guo, Liping; Hu, Chun

    2016-01-01

    Here, we studied the underlying mechanism of aldosterone (Aldo)-induced vascular endothelial cell damages by focusing on ceramide. We confirmed that Aldo (at nmol/L) inhibited human umbilical vein endothelial cells (HUVEC) survival, and induced considerable cell apoptosis. We propose that ceramide (mainly C18) production might be responsible for Aldo-mediated damages in HUVECs. Sphingosine-1-phosphate (S1P), an anti-ceramide lipid, attenuated Aldo-induced ceramide production and following HUVEC damages. On the other hand, the glucosylceramide synthase (GCS) inhibitor PDMP or the ceramide (C6) potentiated Aldo-induced HUVEC apoptosis. Eplerenone, a mineralocorticoid receptor (MR) antagonist, almost completely blocked Aldo-induced C18 ceramide production and HUVEC damages. Molecularly, ceramide synthase 1 (CerS-1) is required for C18 ceramide production by Aldo. Knockdown of CerS-1 by targeted-shRNA inhibited Aldo-induced C18 ceramide production, and protected HUVECs from Aldo. Reversely, CerS-1 overexpression facilitated Aldo-induced C18 ceramide production, and potentiated HUVEC damages. Together, these results suggest that C18 ceramide production mediates Aldo-mediated HUVEC damages. MR and CerS-1 could be the two signaling molecule regulating C18 ceramide production by Aldo. PMID:26788916

  17. 75 FR 54296 - Information Collection; Trends in Use and Users in the Boundary Waters Canoe Area Wilderness, MN

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-07

    ... addressed to Alan E. Watson, Aldo Leopold Wilderness Research Institute, USDA Forest Service Rocky Mountain...: awatson@fs.fed.us . The public may inspect comments received at the Aldo Leopold Wilderness Research.... FOR FURTHER INFORMATION CONTACT: Alan E. Watson, Aldo Leopold Wilderness Research Institute at...

  18. Role of Nongenomic Signaling Pathways Activated by Aldosterone During Cardiac Reperfusion Injury.

    PubMed

    Ashton, Anthony W; Le, Thi Y L; Gomez-Sanchez, Celso E; Morel-Kopp, Marie-Christine; McWhinney, Brett; Hudson, Amanda; Mihailidou, Anastasia S

    2015-08-01

    Aldosterone (Aldo) activates both genomic and nongenomic signaling pathways in the cardiovascular system. Activation of genomic signaling pathways contributes to the adverse cardiac actions of Aldo during reperfusion injury; however, the extent nongenomic signaling pathways contribute has been difficult to identify due to lack of a specific ligand that activates only nongenomic signaling pathways. Using a pegylated aldosterone analog, aldosterone-3-carboxymethoxylamine-TFP ester conjugated to methoxypegylated amine (Aldo-PEG), we are able for the first time to distinguish between nongenomic and genomic cardiac actions of Aldo. We confirm Aldo-PEG activates phosphorylation of ERK1/2 in rat cardiomyocyte H9c2 cells similar to Aldo and G protein-coupled receptor 30 (GPR30 or GPER) agonist G1. GPER antagonist, G36, but not mineralocorticoid receptor (MR) antagonist spironolactone, prevented ERK1/2 phosphorylation by Aldo, Aldo-PEG, and G1. The selective nongenomic actions of Aldo-PEG are confirmed, with Aldo-PEG increasing superoxide production in H9c2 cells to similar levels as Aldo but having no effect on subcellular localization of MR. Striatin serves as a scaffold for GPER and MR, with GPER antagonist G36, but not spironolactone, restoring MR-striatin complexes. Aldo-PEG had no effect on MR-dependent transcriptional activation, whereas Aldo increased transcript levels of serum-regulated kinase 1 and plasminogen activator inhibitor-1. Using our ex vivo experimental rat model of myocardial infarction, we found aggravated infarct size and apoptosis by Aldo but not Aldo-PEG. Our studies confirm that in the heart, activation of nongenomic signaling pathways alone are not sufficient to trigger the deleterious effects of aldosterone during myocardial reperfusion injury. PMID:26121234

  19. COMMITTEES: Committees

    NASA Astrophysics Data System (ADS)

    2010-01-01

    TAUP STEERING COMMITTEE F T Avignone, University of South Carolina B C Barish, CALTECH E Bellotti, University of Milano, INFN J Bernabeu, University of Valencia A Bottino (Chair), University of Torino, INFN N Fornengo, University of Torino, INFN T Kajita, ICRR University of Tokyo C W Kim, Johns Hopkins University, KIAS V Matveev, INR Moscow J Morales, University of Zaragoza G Raffelt, MPI Munchen D Sinclair, University of Carleton M Spiro, IN2P3 TAUP 2009 INTERNATIONAL ADVISORY COMMITTEE J J Aubert, CNRS Marseille M Baldo-Ceolin, University of Padova, INFN G Bellini, University of Milano, INFN L Bergstrom, University of Stockholm R Bernabei, University of Roma Tor Vergata, INFN A Bettini, University of Padova, INFN, LSC S Bilenky, JINR Dubna D O Caldwell, UCSB J Cronin, University of Chicago A Dar, Technion Haifa G Domogatsky, INR Moscow J Ellis, CERN E Fernandez, IFAE Barcelona E Fiorini, University of Milano, INFN T Gaisser, University of Delaware G Gelmini, UCLA G Gerbier, CEA Saclay A Giazotto, INFN Pisa F Halzen, University of Wisconsin W Haxton, University of Washington T Kirsten MPI Heidelberg L Maiani, University of Roma La Sapienza, INFN A McDonald, Queen's University K Nakamura, KEK R Petronzio, INFN, University of Roma Tor Vergata L Resvanis, University of Athens F Ronga INFN, LNF C Rubbia INFN, LNGS A Smirnov, ICTP Trieste C Spiering, DESY N Spooner, University of Sheffield A Suzuki, KEK S Ting MIT, CERN M S Turner, FNAL, University of Chicago J W F Valle, IFIC Valencia D Vignaud, APC Paris G Zatsepin, INR Moscow TAUP 2009 ORGANIZING COMMITTEE R Aloisio, LNGS R Antolini, LNGS F Arneodo, LNGS Z Berezhiani, University of L'Aquila, INFN V Berezinsky, LNGS R Cerulli, LNGS E Coccia [Chair], LNGS/INFN, U of Roma Tor Vergata N D'Ambrosio, LNGS N Fornengo, University of Torino, INFN M Laubenstein, LNGS O Palamara, LNGS L Pandola [Scientific Secretary], LNGS

  20. Mineralocorticoid receptor is involved in the aldosterone pathway in human red blood cells

    PubMed Central

    Bordin, Luciana; Saccardi, Carlo; Donà, Gabriella; Sabbadin, Chiara; Andrisani, Alessandra; Ambrosini, Guido; Plebani, Mario; Brunati, Anna Maria; Ragazzi, Eugenio; Gizzo, Salvatore; Armanini, Decio

    2016-01-01

    We have recently demonstrated that excessive aldosterone (Aldo) secretion in primary aldosteronism (PA) is associated with red blood cells (RBC) senescence. These alterations were prevented/inhibited by cortisol (Cort) or canrenone (Can) raising the hypothesis that Aldo effects in RBC may be mediated by mineralocorticoid receptor (MR), though to date MR has never been demonstrated in human RBC. The aim of this multicenter comparative study was to investigate whether Aldo effects were mediated by MR in these a-nucleated cells. We included 12 healthy controls (HC) and 22 patients with PA. MR presence and activation were evaluated in RBC cytosol by glycerol gradient sedimentation, Western blotting, immuno-precipitation and radioimmunoassay. We demonstrated that RBC contained cytosolic MR, aggregated with HSP90 and other proteins to form multiprotein complex. Aldo induced MR to release from the complex and to form MR dimers which were quickly proteolyzed. Cort induced MR release but not dimers formation while Can was not able to induce MR release. In addition, RBC cytosol from PA patients contained significantly higher amounts of both MR fragments (p<0.0001) and Aldo (p<0.0001) concentrations. In conclusion, in RBC a genomic-like Aldo pathway is proposed involving MR activation, dimerization and proteolysis, but lacking nuclear transcription. In addition, dimers proteolysis may ensure a sort of Aldo scavenging from circulation by entrapping Aldo in MR fragments. PMID:27158328

  1. Mineralocorticoid receptor is involved in the aldosterone pathway in human red blood cells.

    PubMed

    Bordin, Luciana; Saccardi, Carlo; Donà, Gabriella; Sabbadin, Chiara; Andrisani, Alessandra; Ambrosini, Guido; Plebani, Mario; Brunati, Anna Maria; Ragazzi, Eugenio; Gizzo, Salvatore; Armanini, Decio

    2016-01-01

    We have recently demonstrated that excessive aldosterone (Aldo) secretion in primary aldosteronism (PA) is associated with red blood cells (RBC) senescence. These alterations were prevented/inhibited by cortisol (Cort) or canrenone (Can) raising the hypothesis that Aldo effects in RBC may be mediated by mineralocorticoid receptor (MR), though to date MR has never been demonstrated in human RBC. The aim of this multicenter comparative study was to investigate whether Aldo effects were mediated by MR in these a-nucleated cells. We included 12 healthy controls (HC) and 22 patients with PA. MR presence and activation were evaluated in RBC cytosol by glycerol gradient sedimentation, Western blotting, immuno-precipitation and radioimmunoassay. We demonstrated that RBC contained cytosolic MR, aggregated with HSP90 and other proteins to form multiprotein complex. Aldo induced MR to release from the complex and to form MR dimers which were quickly proteolyzed. Cort induced MR release but not dimers formation while Can was not able to induce MR release. In addition, RBC cytosol from PA patients contained significantly higher amounts of both MR fragments (p<0.0001) and Aldo (p<0.0001) concentrations. In conclusion, in RBC a genomic-like Aldo pathway is proposed involving MR activation, dimerization and proteolysis, but lacking nuclear transcription. In addition, dimers proteolysis may ensure a sort of Aldo scavenging from circulation by entrapping Aldo in MR fragments. PMID:27158328

  2. 76 FR 22862 - Submission for OMB Review; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-25

    ... and unconfined types of recreational experiences. The Aldo Leopold Wilderness Research Institute will... a currently valid OMB control number. 30-Day Federal Register Notice Forest Service Title: Aldo Leopold Wilderness Research Institute Wilderness Visitor Study. OMB Control Number: 0596-NEW. Summary...

  3. Age-related changes in thirst, salt appetite, and arterial blood pressure in response to aldosterone-dexamethasone combination in rats

    PubMed Central

    Xue, Baojian; Beltz, Terry G.; Johnson, Alan Kim

    2015-01-01

    This work examined the effects of age on daily water and sodium ingestion and cardiovascular responses to chronic administration of the mineralocorticoid, aldosterone (ALDO) either alone or together with the glucocorticoid, dexamethasone (DEX). Young (4 mo), adult (12 mo), and aged (30 mo) male Brown Norway rats were prepared for continuous telemetry recording of blood pressure (BP) and heart rate (HR). Baseline water and sodium (i.e., 0.3 M NaCl) intake, BP, and HR were established for 10 days. Then ALDO (60 μg/day sc) was infused alone, or together with DEX (2.5 or 20 μg/day sc), for another 10 days. Compared with baseline levels, ALDO stimulated comparable increases in daily saline intake at all ages. ALDO together with the higher dose of DEX (i.e., ALDO/DEX20) increased daily saline intake more than did ALDO, but less so in aged rats. Infusion of ALDO/DEX20 increased mean arterial pressure (MAP), and decreased HR, more than did infusion of ALDO. The changes in MAP in response to both treatments depended on age. For all ages, MAP and saline intake increased simultaneously during ALDO, while MAP always increased before saline intake did during ALDO/DEX20. Contrary to our predictions, MAP did not increase more in old rats in response to either treatment. We speculate that age-related declines in cardiovascular responses to glucocorticoids contributed to the attenuated increases in sodium intake in response to glucocorticoids that were observed in older animals. PMID:25833938

  4. Aldosterone-induced cardiomyocyte growth, and fibroblast migration and proliferation are mediated by TRAF3IP2.

    PubMed

    Somanna, Naveen K; Yariswamy, Manjunath; Garagliano, Joseph M; Siebenlist, Ulrich; Mummidi, Srinivas; Valente, Anthony J; Chandrasekar, Bysani

    2015-10-01

    Sustained activation of the Renin-Angiotensin-Aldosterone System (RAAS) contributes to the pathogenesis of heart failure. Aldosterone (Aldo) is known to induce both myocardial hypertrophy and fibrosis through oxidative stress and proinflammatory pathways. Here we have investigated whether Aldo-mediated cardiomycocyte hypertrophy is dependent on TRAF3IP2, an upstream regulator of IKK and JNK. We also investigated whether the pro-mitogenic and pro-migratory effects of Aldo on cardiac fibroblasts are also mediated by TRAF3IP2. Aldo induced both superoxide and hydrogen peroxide in isolated adult mouse cardiomyocytes (CM), and upregulated TRAF3IP2 expression in part via the mineralocorticoid receptor and oxidative stress. Silencing TRAF3IP2 blunted Aldo-induced IKKβ, p65, JNK, and c-Jun activation, IL-18, IL-6 and CT-1 upregulation, and cardiomyocyte hypertrophy. In isolated adult mouse cardiac fibroblasts (CF), Aldo stimulated TRAF3IP2-dependent IL-18 and IL-6 production, CTGF, collagen I and III expression, MMP2 activation, and proliferation and migration. These in vitro results suggest that TRAF3IP2 may play a causal role in Aldo-induced adverse cardiac remodeling in vivo, and identify TRAF3IP2 as a potential therapeutic target in hypertensive heart disease. PMID:26148936

  5. Mitochondrial reactive oxygen species-mediated NLRP3 inflammasome activation contributes to aldosterone-induced renal tubular cells injury.

    PubMed

    Ding, Wei; Guo, Honglei; Xu, Chengyan; Wang, Bin; Zhang, Minmin; Ding, Feng

    2016-04-01

    Aldosterone (Aldo) is an independent risk factor for chronic kidney disease (CKD), and although Aldo directly induces renal tubular cell injury, the underlying mechanisms remain unclear. NLRP3 inflammasome and mitochondrial reactive oxygen species (ROS) have recently been implicated in various kinds of CKD. The present study hypothesized that mitochondrial ROS and NLRP3 inflammasome mediated Aldo-induced tubular cell injury. The NLRP3 inflammasome is induced by Aldo in a dose- and time-dependent manner, as evidenced by increased NLRP3, ASC, caspase-1, and downstream cytokines, such as interleukin (IL)-1β and IL-18. The activation of the NLRP3 inflammasome was significantly prevented by the selective mineralocorticoid receptor (MR) antagonist eplerenone (EPL) (P < 0.01). Mice harboring genetic knock-out of NLRP3 (NLRP3(-/-)) showed decreased maturation of renal IL-1β and IL-18, reduced renal tubular apoptosis, and improved renal epithelial cell phenotypic alternation, and attenuated renal function in response to Aldo-infusion. In addition, mitochondrial ROS was also increased in Aldo-stimulated HK-2 cells, as assessed by MitoSOXTM red reagent. Mito-Tempo, the mitochondria-targeted antioxidant, significantly decreased HK-2 cell apoptosis, oxidative stress, and the activation of NLRP3 inflammasome. We conclude that Aldo induces renal tubular cell injury via MR dependent, mitochondrial ROS-mediated NLRP3 inflammasome activation. PMID:27014913

  6. Managing for resilience

    EPA Science Inventory

    Early efforts in wildlife management focused on reducing population variability and maximizing yields of select species. Aldo Leopold proposed the concept of habitat management as superior to population management. More recently, ecosystem management, whereby ecological processes...

  7. Roles of aldosterone in vascular calcification: An update.

    PubMed

    Gao, Jingwei; Zhang, Kun; Chen, Jie; Wang, Mong-Heng; Wang, Jingfeng; Liu, Pinming; Huang, Hui

    2016-09-01

    Both clinical and experimental studies have demonstrated that vascular calcification (VC) is a common pathology shared in many chronic diseases such as chronic kidney disease (CKD) and diabetes. It's an independent risk factor for cardiovascular events. Since the pathogenesis of VC is complicated, current therapies have limited effects on the regression of VC. Therefore, it is urgent to investigate the potential mechanisms and find new targets for the treatment of VC. Aldosterone (Aldo), a mineralocorticoid hormone, is the metabolite of renin-angiotensin-aldosterone system (RAAS) activation, which can exert genomic and non-genomic effects on the cardiovascular system. Recent data suggests that Aldo can promote VC. Here, we summarized the roles of Aldo in the process of VC and a series of findings indicated that Aldo could act as a potentially therapeutic target for treating VC. PMID:27238972

  8. The role of mineralocorticoid action in the brain in salt-sensitive hypertension

    PubMed Central

    Oki, Kenji; Gomez-Sanchez, Elise P.; Gomez-Sanchez, Celso E.

    2011-01-01

    Summary 1. The mechanisms by which excessive salt causes hypertension involve more than retention of sodium and water by the kidneys and are far from clear. Mineralocorticoids act centrally to increase salt appetite, sympathetic drive and vasopressin release, resulting in hypertension that is prevented by the central infusion of mineralocorticoid receptor (MR) antagonists. The MR has similar affinity for aldo and the glucocorticoids corticosterone or cortisol. Specificity is conferred in transport epithelia by the co-localization of the MR with 11β-hydroxysteroid dehydrogenase type 2. Co-expression also occurs in some neurons, notably those of the nucleus tractus solitarius that are activated by sodium depletion and aldo and mediate salt seeking behavior. 2. The salt-induced hypertension of the Dahl Salt Sensitive rat is mitigated by the central infusion of a mineralocorticoid antagonist even though circulating aldo is normal or reduced in SS. Contrary to reports that salt appetite in the SS rat is depressed, we found that it is increased compared to the Spraque-Dawley (SD). 3. Extra-adrenal aldo synthesis in the brain occurs in minute amounts that could only be relevant locally. mRNA for aldo synthase enzyme and aldo concentration in the brain of the Dahl SS are increased compared to SD rats. The central infusion of inhibitors of aldo synthesis lowers salt-induced hypertension in the SS, suggesting a role for excessive aldo synthesis in the brain. Brain MR, particularly those in the PVN, regulate inflammatory processes that are exacerbated by sodium and lead to cardiovascular dysfunction. PMID:21585422

  9. Aldosterone induces NRK-52E cell apoptosis in acute kidney injury via rno-miR-203 hypermethylation and Kim-1 upregulation

    PubMed Central

    Xiao, Xiangcheng; Tang, Rong; Zhou, Xiao; Peng, Ling; Yu, Pingping

    2016-01-01

    Acute kidney injury (AKI) is characterized by an acute reduction in kidney function as identified by an increase in serum creatinine levels and reduction in urine output. Kidney injury molecule-1 (Kim-1) is a hallmark of kidney diseases, since it is typically non-detectable in the non-injured kidney, but upregulated and excreted in the urine during AKI. Aldosterone (Aldo) is a mediator of the renin-angiotensin-Aldo system with a pivotal role in the regulation of salt and extracellular fluid metabolism. In the present study, mice subjected to renal ischemia/reperfusion-induced AKI were investigated. The mice exhibited elevated levels of Aldo and angiotensin II, together with increased Kim-1 expression levels in renal tissue. Treatment of the mice with the Aldo receptor antagonist spironolactone decreased Kim-1 expression levels. These results suggest that Aldo may be associated with the expression of Kim-1 during AKI. However, the molecular mechanism underlying the role of Aldo in Kim-1 expression is unclear, and thus was investigated using NRK-52E cells. Aldo was found to induce the apoptosis of NRK-52E cells via the hypermethylation of rno-microRNA (miR)-203 and upregulation of Kim-1. In addition, luciferase reporter assays demonstrated that Kim-1 was a target gene of rno-miR-203 in NRK-52E cells. Furthermore, Aldo-induced NRK-52E cell apoptosis was reduced by treatment with pre-miR-203 and spironolactone to a greater extent when compared with either alone. The results may provide a promising diagnostic marker or novel therapeutic target for AKI. PMID:27446296

  10. mPGES-1 deletion impairs aldosterone escape and enhances sodium appetite

    PubMed Central

    Jia, Zhanjun; Aoyagi, Toshinori; Kohan, Donald E.

    2010-01-01

    Aldosterone (Aldo) is a major sodium-retaining hormone that reduces renal sodium excretion and also stimulates sodium appetite. In the face of excess Aldo, the sodium-retaining action of this steroid is overridden by an adaptive regulatory mechanism, a phenomenon termed Aldo escape. The underlying mechanism of this phenomenon is not well defined but appeared to involve a number of natriuretic factors such prostaglandins (PGs). Here, we investigated the role of microsomal prostaglandin E synthase-1 (mPGES-1) in the response to excess Aldo. A 14-day Aldo infusion at 0.35 mg·kg−1·day−1 via an osmotic minipump in conjunction with normal salt intake did not produce obvious disturbances in fluid metabolism in WT mice as suggested by normal sodium and water balance, plasma sodium concentration, hematocrit, and body weight, despite the evidence of a transient sodium accumulation on days 1 or 2. In a sharp contrast, the 14-day Aldo treatment in mPGES-1 knockoute (KO) mice led to increased sodium and water balance, persistent reduction of hematocrit, hypernatremia, and body weight gain, all evidence of fluid retention. The escaped wild-type (WT) mice displayed a remarkable increase in urinary PGE2 excretion in parallel with coinduction of mPGES-1 in the proximal tubules, accompanied by a remarkable, widespread downregulation of renal sodium and water transporters. The increase in urinary PGE2 excretion together with the downregulation of renal sodium and water transporters were all significantly blocked in the KO mice. Interestingly, compared with WT controls, the KO mice exhibited consistent increases in sodium and water intake during Aldo infusion. Together, these results suggest an important role of mPGES-1 in antagonizing the sodium-retaining action of Aldo at the levels of both the central nervous system and the kidney. PMID:20335314

  11. Mitochondrial reactive oxygen species-mediated NLRP3 inflammasome activation contributes to aldosterone-induced renal tubular cells injury

    PubMed Central

    Ding, Wei; Guo, Honglei; Xu, Chengyan; Wang, Bin; Zhang, Minmin; Ding, Feng

    2016-01-01

    Aldosterone (Aldo) is an independent risk factor for chronic kidney disease (CKD), and although Aldo directly induces renal tubular cell injury, the underlying mechanisms remain unclear. NLRP3 inflammasome and mitochondrial reactive oxygen species (ROS) have recently been implicated in various kinds of CKD. The present study hypothesized that mitochondrial ROS and NLRP3 inflammasome mediated Aldo–induced tubular cell injury. The NLRP3 inflammasome is induced by Aldo in a dose- and time-dependent manner, as evidenced by increased NLRP3, ASC, caspase-1, and downstream cytokines, such as interleukin (IL)-1β and IL-18. The activation of the NLRP3 inflammasome was significantly prevented by the selective mineralocorticoid receptor (MR) antagonist eplerenone (EPL) (P < 0.01). Mice harboring genetic knock-out of NLRP3 (NLRP3−/−) showed decreased maturation of renal IL-1β and IL-18, reduced renal tubular apoptosis, and improved renal epithelial cell phenotypic alternation, and attenuated renal function in response to Aldo-infusion. In addition, mitochondrial ROS was also increased in Aldo-stimulated HK-2 cells, as assessed by MitoSOXTM red reagent. Mito-Tempo, the mitochondria-targeted antioxidant, significantly decreased HK-2 cell apoptosis, oxidative stress, and the activation of NLRP3 inflammasome. We conclude that Aldo induces renal tubular cell injury via MR dependent, mitochondrial ROS-mediated NLRP3 inflammasome activation. PMID:27014913

  12. PGC-1α overexpression protects against aldosterone-induced podocyte depletion: role of mitochondria

    PubMed Central

    Zhao, Min; Yuan, Yanggang; Bai, Mi; Ding, Guixia; Jia, Zhanjun; Huang, Songming; Zhang, Aihua

    2016-01-01

    Growing evidence has shown that podocyte number is a critical determinant for the development of glomerulosclerosis and progressive renal failure. We previously reported that mitochondrial dysfunction (MtD) is an early event in podocyte injury. Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) is an important modulator of mitochondrial biogenesis. Here, we investigated the role of PGC-1α overexpression in podocyte depletion and the involvement of mitochondria in this process. Following chronic aldosterone (Aldo) infusion for 14 days, we observed a remarkable podocyte loss, podocyte phenotypic changes, and albuminuria in WT mice. However, all these abnormalities were significantly attenuated in PGC-1α transgenic mice. Next, we examined mitochondrial function in both genotypes with or without Aldo infusion. As expected, Aldo-induced MtD in glomeruli was markedly improved in PGC-1α transgenic mice. In vitro, Aldo induced podocyte detachment and phenotypic changes in line with MtD in dose- and time-dependent manners. Similarly, ethidium bromide, an inducer of MtD, mimicked Aldo effects on podocyte detachment and phenotypic alterations. Notably, overexpression of PGC-1α in podocytes entirely reversed Aldo-induced podocyte detachment, phenotypic changes, and MtD. Taken together, these findings demonstrate that PGC-1α protects against podocyte depletion and phenotypic changes possibly by maintaining normal mitochondrial function. PMID:26943584

  13. Taphophobia and 'life preserving coffins' in the nineteenth century.

    PubMed

    Cascella, Marco

    2016-09-01

    In 1891 the Italian psychiatrist Enrico Morselli (1852-1929) described taphophobia, defining it as an extreme condition of claustrophobia due to the fear of being buried alive. This rare psychopathological phenomenon reflects an ancient fear, and its origin is not known. Taphophobia is closely linked to the problem of apparent death and premature burial. In the nineteenth century, scientists and authors paid particular attention to the issue of apparent death, and special devices (safety coffins) were invented to ensure that premature burial was avoided. Nowadays taphophobia is quite a rare psychiatric disorder; different forms of social anxiety disorders are much more widespread. Its modern equivalent could be the fear of organs harvested from a patient who is still alive. PMID:27225418

  14. Low Na, High K Diet and the Role of Aldosterone in BK-Mediated K Excretion

    PubMed Central

    Cornelius, Ryan J.; Wen, Donghai; Li, Huaqing; Yuan, Yang; Wang-France, Jun; Warner, Paige C.; Sansom, Steven C.

    2015-01-01

    A low Na, high K diet (LNaHK) is associated with a low rate of cardiovascular (CV) disease in many societies. Part of the benefit of LNaHK relies on its diuretic effects; however, the role of aldosterone (aldo) in the diuresis is not understood. LNaHK mice exhibit an increase in renal K secretion that is dependent on the large, Ca-activated K channel, (BK-α with accessory BK-β4; BK-α/β4). We hypothesized that aldo causes an osmotic diuresis by increasing BK-α/β4-mediated K secretion in LNaHK mice. We found that the plasma aldo concentration (P[aldo]) was elevated by 10-fold in LNaHK mice compared with control diet (Con) mice. We subjected LNaHK mice to either sham surgery (sham), adrenalectomy (ADX) with low aldo replacement (ADX-LA), or ADX with high aldo replacement (ADX-HA). Compared to sham, the urinary flow, K excretion rate, transtubular K gradient (TTKG), and BK-α and BK-β4 expressions, were decreased in ADX-LA, but not different in ADX-HA. BK-β4 knockout (β4KO) and WT mice exhibited similar K clearance and TTKG in the ADX-LA groups; however, in sham and ADX-HA, the K clearance and TTKG of β4KO were less than WT. In response to amiloride treatment, the osmolar clearance was increased in WT Con, decreased in WT LNaHK, and unchanged in β4KO LNaHK. These data show that the high P[aldo] of LNaHK mice is necessary to generate a high rate of BK-α/β4-mediated K secretion, which creates an osmotic diuresis that may contribute to a reduction in CV disease. PMID:25607984

  15. Time-dependent aldosterone metabolism in toad urinary bladder

    SciTech Connect

    Brem, A.S.; Pacholski, M.; Morris, D.J.

    1988-04-01

    Aldosterone (Aldo) metabolism was examined in the toad bladder. Bladders were incubated with (/sup 3/H)aldosterone (10(-7) M) for 5 h, 1 h, or 10 min. Tissues were analyzed for metabolites using high-pressure liquid chromatography (HPLC). In separate experiments, Na+ transport was assessed by the short-circuit current (SCC) technique. Following a 5-h tissue incubation, about 25% of the (/sup 3/H)-aldosterone was converted into metabolites including a polar monosulfate metabolite, 20 beta-dihydroaldo (20 beta-DHAldo), small quantities of 5 beta-reduced products, and a variety of 5 alpha-reduced Aldo products including 5 alpha-DHAldo, 3 alpha,5 alpha-tetrahydroaldo (3 alpha,5 alpha-THAldo), and 3 beta,5 alpha-THAldo. Tissues metabolized approximately 10% of the labeled hormone into the same compounds by 1 h. Measurable quantities of these metabolites were also synthesized by bladders exposed to Aldo for only 10 min and then incubated in buffer for an additional 50 min without Aldo. Bladders pretreated with the spironolactone, K+-canrenoate (3.5 X 10(-4) M), and stimulated with Aldo (10(-7) M) generated a peak SCC 44 +/- 6% of that observed in matched pairs stimulated with Aldo (P less than 0.001; n = 6). K+-canrenoate also markedly diminished (/sup 3/H)aldosterone metabolism at both 5 and 1 h. Thus, metabolic transformation of Aldo begins prior to hormone-induced increases in Na+ transport. Both the generation of certain metabolites (e.g., 5 alpha-reductase pathway products) and the increase in Na+ transport can be selectively inhibited by K+-canrenoate.

  16. Aldosterone induces myofibroblast EGF secretion to regulate epithelial colonic permeability.

    PubMed

    Miró, Lluïsa; Pérez-Bosque, Anna; Maijó, Mònica; Amat, Concepció; Naftalin, Richard J; Moretó, Miquel

    2013-05-01

    In vivo studies show that raised aldosterone (Aldo) during low-Na adaptation regulates the growth of pericryptal myofibroblasts and reduces the permeability of the colonic epithelium. The aim of this study was to reproduce in vitro the in vivo condition of increased Aldo using human CCD-18Co myofibroblasts and T84 colonic epithelial cells to measure myofibroblast and epithelial proliferation and the expression of intercellular junction proteins. Proliferation was quantified by measuring 5-bromo-2'-deoxyuridine incorporation. The myofibroblast expression of EGF, VEGFa, and transforming growth factor-β1 (TGF-β1) was measured by real-time PCR and the expression of junctional complex proteins by Western blot. Aldo stimulated the proliferation of myofibroblasts by 70% (P < 0.05) and increased EGF mRNA expression by 30% (P < 0.05) without affecting VEGFa and TGF-β1. EGF concentration in the incubation medium increased by 30% (P < 0.05) 24 h after Aldo addition, and these effects were prevented by the addition of spironolactone. Myofibroblast proliferation in response to Aldo was mediated by EGF receptor (EGFR) and involved both MAPKK and phosphatidylinositol 3-kinase pathways. When T84 cells were incubated with medium from myofibroblasts stimulated with Aldo (conditioned medium), the expression of β-catenin and claudin IV was increased by 30% (P < 0.05) and proliferation by 40% (P < 0.05). T84 proliferation decreased when α-EGF, or the EGFR antagonist AG1478, was present. Results in vivo indicate that rats fed a low-salt diet showed an increased expression of EGF and EGFR in the colonic mucosa. These results support the view that changes in colonic permeability during low-Na adaptation are mediated by the EGF secreted by myofibroblasts in response to raised Aldo. PMID:23467299

  17. Aldosterone receptor blockers spironolactone and canrenone: two multivalent drugs.

    PubMed

    Armanini, Decio; Sabbadin, Chiara; Donà, Gabriella; Clari, Giulio; Bordin, Luciana

    2014-05-01

    Canrenone is a derivative of spironolactone with lower antiandrogen activity. The drug is used only in few countries and can block all the side effects of aldosterone (ALDO). The drug is effective even in the presence of normal concentrations of ALDO. Mineralcorticoid receptor antagonists block the inflammatory activity of ALDO at the level of target tissues as heart, vessels and mononuclear leukocytes. Canrenone reduces the progression of insulin resistance and of microalbuminuria in type 2 diabetes and other related diseases. Both canrenone and hydrochlorothiazide can enhance the effect of treatment with ACE inhibitors and angiotensin II receptor blockers on microalbuminuria, but ALDO receptor blockers are more active. This different action is due to the fact that only canrenone blocks mineralocorticoid receptors. Serum potassium and renal function should be monitored before and during the treatment. ALDO receptor blockers are recommended in addition to polytherapy for resistant hypertension, but there are no studies on the effect of the drug as first-choice therapy. PMID:24617854

  18. Acknowledgements

    NASA Astrophysics Data System (ADS)

    2016-07-01

    The Meeting was sponsored by the Istituto Nazionale di Fisica Nucleare (INFN), the Società Italiana di Fisica (SIF), the European Physics Society (EPS), the University of Pisa and the University of Siena.

  19. Highlights from Fermilab

    NASA Astrophysics Data System (ADS)

    Oddone, P. J.

    2010-12-01

    DISCUSSION by CHAIRMAN: P.J. ODDONE, Scientific Secretaries: W. Fisher, A. Holzner Note from Publisher: The Slides of the Lecture: "Highlights from Fermilab" can be found at http://www.ccsem.infn.it/issp2007/

  20. Status And Perspectives In Italian HEP With Accelerators

    SciTech Connect

    Ferroni, Fernando

    2005-10-12

    In this paper I give an overview on the status of elementary particle physics research performed with accelerators in INFN . Recent successes, potential problems and a look to the future are discussed.

  1. MONOLITH: a massive magnetized iron detector for atmospheric neutrinos

    NASA Astrophysics Data System (ADS)

    MONOLITH Collaboration

    2001-08-01

    The MONOLITH (Massive Observatory for Neutrino Oscillation or LImits on THeir existence) project is a proposal (N.Y. Agafonova et al., 2000) for an experiment to be installed in the Gran Sasso underground laboratory to study atmospheric neutrino oscillations with a massive magnetized iron tracking calorimeter . The main purpose is to confirm the existence of atmospheric neutrino oscillations through the explicit observation of the first oscillation minimum in νµ disappearance. The MONOLITH detector has been designed in order to discriminate among different oscillation modes and to accurately measure the oscillation parameters in a range that completely covers the Super-Kamiokande allowed region. Other measurements include studies of matter effects, the NC up down ratio, the ¯ν/ν ratio, the study of cosmic ray muons in the multi-TeV energy region, and auxiliary measurements from the CERN to Gran Sasso neutrino beam. Correspondence to: G. C. Trinchero (trinchero@to.infn.it) 1 Institutions participating to the MONOLITH Collaboration: INFN, Sezione di Napoli, Napoli, Italy Moscow Engineering Physics Insitute, Moscow, Russia Universit´a di Bologna and INFN, Bologna, Italy Columbia University, New York, USA Laboratori Nazionali di Frascati, INFN, Frascati, Italy Universit´a di Torino,Torino, Italy Universit`a di Milano Bicocca and INFN Sezione di Milano, Italy M¨unster University, M¨unster, Germany Hamburg University, Hamburg, Germany INFN, Sezione di Torino, Torino, Italy Istituto di Cosmogeofisica, CNR, Torino, Italy Institute for Nuclear Research (INR), Moscow, Russia Laboratori Nazionali del Gran Sasso, INFN, Assergi, Italy Universit`a de L'Aquila and INFN, L'Aquila, Italy Universit´a di Roma, Roma, Italy Bonn University, Bonn, Germany Humboldt University Berlin, Berlin, Germany

  2. Silicon Microstrip Detectors for the Jlab SBS Spectrometer

    NASA Astrophysics Data System (ADS)

    de Persio, F.; Kiprich, S.; Meddi, F.; Urciuoli, G. M.

    2014-06-01

    The INFN group of Rome is developing two silicon microstrip detector planes to be part of the tracking system of the SBS spectrometer, that will be installed in the experimental Hall A of Jefferson Labortatory, in order to improve its resolution. The detector and the PCB design were the results of models simulated using PSPICE. The entire assembly process will be realized in the INFN Roma clean room CL10000 facility.

  3. Technical specification for the 1.5 Tesla superconducting solenoid for the BaBar detector. Revision 1

    SciTech Connect

    O`Connor, T.G.; Bell, R.; Fabbricatore, P.; Giorgi, M.; Hitlin, D.

    1997-03-07

    This document sets forth the specification of the BABAR superconducting solenoid and power supply which is being supplied to the BABAR collaboration by INSTITUTO NAZIONALE DI FISICA NUCLEARE (INFN). The solenoid will be installed in the BABAR detector which will be located at Interaction Region 2 (IR2) of the PEP II machine, a positron electron collider, presently under construction at the Stanford Linear Accelerator Center (SLAC) located in Menlo Park, California. The solenoid will become part of the BABAR detector which will be used in SLAC`s high energy physics program. Intense beams of electrons and positrons are made to collide inside the solenoid magnet. High field uniformity quality, precise mechanical alignment and long term stability are essential characteristics of the solenoid. INFN will set up a committee that will provide contractual and technical oversight throughout the design, fabrication and installation phases of the BABAR solenoid construction. That committee will be the final authority to resolve any differences between these specifications and the INFN supplied drawings, in addition to any differences between these specifications or the INFN supplied drawings and the proposals from the vendor. All submissions for approval to INFN whether for design changes, material approval, design submissions or others as required by this specification shall be acted upon INFN within two (2) weeks of receipt of the submissions. If no answer is given the vendor may assume approval and proceed.

  4. Nature's Web: Caring for the Land. National Wildlife Week Educator's Guide, April 19-25, 1998 = Nature's Web: El Cuidado de la Tierra. National Wildlife Week Guia para el Educador, April 19-25, 1998.

    ERIC Educational Resources Information Center

    Tunstall, Margaret; Kier, Jennifer; Dixon, Cheryl; Bradley, Sara; Hodges, Elenor; Levey, Sharon

    This guide features Aldo Leopold's land ethic woven into a series of activities that also represent the five core issues of most concern to the National Wildlife Federation (NWF): (1) wetlands; (2) water quality; (3) land stewardship; (4) endangered habitats; and (5) sustainable communities. Each activity is introduced by a biographical sketch of…

  5. LC/MSMS STUDY OF BENZO[A]PYRENE-7,8-QUINONE ADDUCTION TO GLOBIN TRYPTIC PEPTIDES AND N-ACETYLAMINO ACIDS

    EPA Science Inventory

    Benzo[a]pyrene-7,8-quinone (BPQ) is regarded as a reactive genotoxic compound enzymatically formed from a xenobiotic precursor benzo[a]pyrene-7,8-diol by aldo-keto-reductase family of enzymes. Because BPQ, a Michael electrophile, was previously shown to react with oligonucleotide...

  6. CHARACTERIZATION OF STABLE BENZOLALPYRENE-7,8-QUINONE-DNA ADDUCTS IN CALF THYMUS DNA AND POLYDEOXYNUCLEOTIDES

    EPA Science Inventory

    Bcnzo[a]pyrene-7,8-dione (BPQ) is a reactive aldo-keto reductase-mediated product of B[a]P-7,8-diol, a major P450/epoxide hydrolase metabolite of the multi-species carcinogen, B[a]P. The role of BPQ in B[a]P's genotoxicity and carcinogenesis is evolving. Toxicity pathways involvi...

  7. Monitoring invasive species using 1-mm GSD geocoded aerial surveys: a cost effective means of getting details, locations, and sample numbers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Half a century ago Aldo Leopold observed, “It is impossible fully to protect cheat country from fire...,” and chided, “We tilt windmills in behalf of conservation in convention halls... but on the back forty we disclaim even owning a lance.” “Lances” effective against cheatgrass (Bromus tectorum L.)...

  8. Linking biological conservation to healthy rural communities: a case history of the Janos – Casas Grandes Biosphere Reserve

    Technology Transfer Automated Retrieval System (TEKTRAN)

    During the late 1930’s, Aldo Leopold witnessed a striking contrast along the narrow boundary between the United States and Mexico. He later described Mexican ecosystems as a “lovely picture of ecological health” and those same ecosystems north of the U.S. - Mexico border as “so badly damaged that on...

  9. Human carbonyl reductase (CBR) localized to band 21q22. 1 by high-resolution fluorescence in situ hybridization displays gene dosage effects in trisomy 21 cells

    SciTech Connect

    Lemieux, N. ); Malfoy, B. ); Forrest, G.L. )

    1993-01-01

    Human carbonyl reductase (CBR) belongs to a group of NADPH-dependent enzymes called aldo-keto reductases. The enzyme can function as an aldo-keto reductase or as a quinone reductase with potential for modulating quinone-mediated oxygen free radicals. The CBR gene was mapped by high-resolution fluorescence in situ hybridization to band 21q22.12, very close to the SOD1 locus at position 2lq22.11. CBR displayed gene dosage effects in trisomy 21 human lymphoblasts at the DNA and mRNA levels. Lymphoblasts with increasing chromosome 21 ploidy also showed increased aldo-keto reductase activity and increased quinone reductase activity. Both aldo-keto reductase activity and quinone reductase activity have been shown to be associated with carbonyl reductase. The location of CBR near SOD1 and the increased enzyme activity and potential for free radical modulation in trisomy 21 cells implicate CBR as a candidate for contributing to the pathology of certain diseases such as Down syndrome and Alzheimer disease. 28 refs., 1 fig., 1 tab.

  10. CHARACTERIZATION OF STABLE BENZO(A)PYRENE-7,8-QUINONE-DNA ADDUCTS IN CALF THYMUS DNA

    EPA Science Inventory

    Benzo[alpyrene-7,8-dione (BPQ) is a reactive aldo-keto reductase-mediated product of B[a]P-7,8-diol, a major P450/epoxide hydrolase metabolite of the multi-species carcinogen, B[a]P. The role of BPQ in B[a]P's genotoxicity and carcinogenesis is evolving. Toxicity pathways involvi...

  11. Dead Wolves, Dead Birds, and Dead Trees: Catalysts for Transformative Learning in the Making of Scientist-Environmentalists

    ERIC Educational Resources Information Center

    Walter, Pierre

    2013-01-01

    This historical study identifies catalysts for transformative learning in the lives of three scientist-environmentalists important to the 20th-century environmental movement: Aldo Leopold, Rachel Carson, and David Suzuki. Following a brief review of theoretical perspectives on transformative learning, the article argues that transformative…

  12. From Romanticism to Deep Ecology: The Continuing Evolution in American Environmental Thought.

    ERIC Educational Resources Information Center

    Ackerson, David

    2000-01-01

    Describes the contributions to deep ecology of Henry Thoreau, who advocated acting upon strongly held convictions; John Muir, who adopted a biocentric view of nature; and Aldo Leopold, who formulated an egalitarian ecosystem ethic. While deep ecology is moving toward a new vision of humankind's relation to nature, it has yet to coalesce into a…

  13. Environmental Literacy in America: What Ten Years of NEETF/Roper Research and Related Studies Say about Environmental Literacy in the U.S.

    ERIC Educational Resources Information Center

    Coyle, Kevin

    2005-01-01

    In 1944, noted conservationist Aldo Leopold wrote: "Acts of conservation without the requisite desires and skill are futile. To create these desires and skills, and the community motive, is the task of education." Almost sixty years later, in January 2003, the National Science Foundation released a report of its Advisory Committee for…

  14. Putting History at the Core: History and Literature in Environmental Studies

    ERIC Educational Resources Information Center

    Morse, Kathryn

    2003-01-01

    When environmental studies programs broaden their curricular offerings into the humanities, their first stop is often environmental literature, particularly classics such as Henry David Thoreau's "Walden," Aldo Leopold's "A Sand County Almanac," and Rachel Carson's "Silent Spring." Environmental literature courses consider many of the works of…

  15. Adventures with Doug: An Interview with Dot Wade, Prairie Botanist.

    ERIC Educational Resources Information Center

    Wiener, Morris; Wade, Dot

    1995-01-01

    The wife of the late Doug Wade discusses the course of his career in outdoor education and nature interpretation; the relationship he had with his mentor, Aldo Leopold; his success as a teacher and his relationship with his students; and how a background in botany enabled her to assist her husband with field trips. (LP)

  16. Productivism and the Product Paradigm in Technological Education

    ERIC Educational Resources Information Center

    Elshof, Leo

    2006-01-01

    Aldo Leopold was a pioneer in United States' wildlife management and his axiom is certainly familiar to anyone who has tried to repair a complex technological system; it applies equally whether one is rebuilding a small block V8 engine or repairing a computer. "Keep all the pieces" is good advice because in all likelihood they will all…

  17. Landfullness in Adventure-Based Programming: Promoting Reconnection to the Land

    ERIC Educational Resources Information Center

    Baker, Molly

    2005-01-01

    Nearly a half-century ago. Aldo Leopold acknowledged the threat of "landlessness" in our society as measured by the loss of our collective awareness of, and admiration for, the land (Leopold. 1966). At present, this disconnect has evolved to the point where participants in adventure-based programs may find themselves traveling through "Any Woods,…

  18. Caring for Nature 101, or Alternative Perspectives on Educating Natural Resource Managers and Ecologically Conscious Citizens.

    ERIC Educational Resources Information Center

    Booth, Annie L.

    1998-01-01

    Examines the contributions of alternative perspectives from environmental philosophy to university teaching practices that address the question of how to produce ecologically educated citizens. Considers the challenges to modern resource education found in feminist and ecofeminist philosophies and in Aldo Leopold's "Land Ethic." Discusses…

  19. Eagle Heights Woods: Man's Use of Land.

    ERIC Educational Resources Information Center

    Pratt, Cay; Nelson, Redgy

    This teacher's guide for use in the elementary grades was prepared as a result of the 1970 Local Materials Workshop on Outdoor Education, Madison, Wisconsin. It develops the concept of a land ethnic as expressed by Aldo Leopold in "A Sand County Almanac". A filmstrip is employed to discover the meaning of several words pertinent to…

  20. Aldosterone response to sodium deprivation and angiotensin II in patients with hypopituitarism.

    PubMed

    Seifert, C; Oelkers, W

    1981-03-01

    Unknown pituitary factor(s) apart from ACTH may participate in the regulation of aldosterone (aldo) secretion in man. We investigated whether the 'sensitization' of the zona glomerulosa against angiotensin II (A II) by sodium deficiency was mediated by the pituitary gland. A II was infused in stepwise increasing doses (2, 4, 8 ng/kg/min) into 5 normal subjects (N) and into 8 patients with hypopituitarism (H) before and after 4 days on low sodium diet. Mean cumulative sodium balance after the low sodium diet was -145mM in N and -165mM in H. Plasma-aldo and aldo-excretion rate on the normal sodium diet were slightly higher in H than in N but rose less than normal during sodium depletion in H. Plasma A II and renin activity on normal sodium were slightly higher in H than in N, but the increase on the low sodium diet was blunted in H. The stimulation of plasma-aldo by A II infusion was similar in both groups on the normal sodium diet. In both groups, the response of P-aldo to A II infusion was greater in the sodium deplete than in the replete state, although 'sensitization' was slightly less marked in H than in N. This may be due to the blunted rise of plasma-A II after sodium loss in H, which may also account for abnormalities in the blood pressure response in the H group. Altogether, the results speak against a direct involvement of the pituitary gland in 'sensitization', but an indirect influence through unexplained abnormalities in renin secretion is possible. PMID:7211097

  1. Changes in plasma angiotensin II, aldosterone, arginine vasotocin, corticosterone, and electrolyte concentrations during acclimation to dry condition and seawater in the crab-eating frog.

    PubMed

    Uchiyama, Minoru; Maejima, Sho; Wong, Marty K S; Preyavichyapugdee, Narin; Wanichanon, Chaitip; Hyodo, Susumu; Takei, Yoshio; Matuda, Kouhei

    2014-01-01

    The crab-eating frog Fejervarya cancrivora inhabits mangrove swamps and marshes in Southeast Asia. In the present study, circulating angiotensin II (Ang II), aldosterone (Aldo), arginine vasotocin (AVT), and corticosterone (Cort) concentrations as well as various blood parameters were studied under osmotically stressful conditions. Following acclimation to hyperosmotic seawater and dry condition for 5days, body weight was significantly decreased. Under both conditions, plasma Na(+), Cl(-), and urea concentrations, hematocrit values (Ht; blood volume indicator), and osmolality were significantly increased. Dehydration associated with hypovolemic and hyperosmotic states of body fluids was induced during acclimation to hyperosmotic seawater and dry condition in the crab-eating frogs. Ang II, Aldo, AVT, and Cort were maintained within relatively narrow concentration ranges in the control frogs; however, in frogs under dry and hyperosmotic seawater conditions, large variations were observed among individuals in each group. Mean plasma Ang II and Aldo concentrations significantly increased in hyperosmotic seawater-acclimated and desiccated frogs. Although mean plasma AVT concentrations in dehydrated frogs of both the groups were approximately 2.0-3.5 times higher than those in the control frogs, the differences were not significant because of the variation. There was a significant correlation between plasma osmolality and AVT as well as Ang II but not Aldo. A significant correlation was also observed between Ht and AVT as well as Ang II. Plasma Ang II was significantly correlated with plasma Aldo. These results indicate that the crab-eating frogs may exhibit similar physiological responses to both seawater-acclimated and dry conditions. It appears that under dehydrated conditions, osmoregulatory mechanisms participate in stabilization of the situation. The renin-angiotensin system may have pivotal roles in body fluid regulation under volemic and osmotic stress in the

  2. WNoDeS, a tool for integrated Grid and Cloud access and computing farm virtualization

    NASA Astrophysics Data System (ADS)

    Salomoni, Davide; Italiano, Alessandro; Ronchieri, Elisabetta

    2011-12-01

    INFN CNAF is the National Computing Center, located in Bologna, Italy, of the Italian National Institute for Nuclear Physics (INFN). INFN CNAF, also called the INFN Tier-1, provides computing and storage facilities to the International High-Energy Physics community and to several multi-disciplinary experiments. Currently, the INFN Tier-1 supports more than twenty different collaborations; in this context, optimization of the usage of computing resources is essential. This is one of the main drivers behind the development of a software called WNoDeS (Worker Nodes on Demand Service). WNoDeS, developed at INFN CNAF and deployed on the INFN Tier-1 production infrastructure, is a solution to virtualize computing resources and to make them available through local, Grid or Cloud interfaces. It is designed to be fully integrated with a Local Resource Management System; it is therefore inherently scalable and permits full integration with existing scheduling, policing, monitoring, accounting and security workflows. WNoDeS dynamically instantiates Virtual Machines (VMs) on-demand, i.e. only when the need arises; these VMs can be tailored and used for purposes like batch job execution, interactive analysis or service instantiation. WNoDeS supports interaction with user requests through traditional batch or Grid jobs and also via the Open Cloud Computing Interface standard, making it possible to allocate compute, storage and network resources on a pay-as-you-go basis. User authentication is supported via several authentication methods, while authorization policies are handled via gLite Argus. WNoDeS is an ambitious solution aimed at virtualizing cluster resources in medium or large scale computing centers, with up to several thousands of Virtual Machines up and running at any given time. In this paper, we descrive the WNoDeS architecture.

  3. The origins of psychology in Italy: Themes and authors that emerge through a content analysis of the Rivista di Filosofia Scientifica [Journal of Scientific Philosophy].

    PubMed

    Bartolucci, Chiara; Lombardo, Giovanni Pietro

    2012-08-01

    This article examines the scientific-cultural context of the second half of the 1800s, during which psychological science emerged in Italy. The article explores the contribution made by the emergence of the primary research traditions of that period, namely, physiological anthropology and phreniatry, by means of a methodology that combines content analysis with a classical historiographical study of the period. Themes and authors deriving from the various disciplines in the human and natural sciences were identified through a content analysis of the Rivista di Filosofia Scientifica [Journal of Scientific Philosophy], a periodical that is representative of Italian positivism. The analysis highlights the epistemological perspective held by scholars who, distancing themselves from the mechanistic reductionism of the proponents of positivism, integrated a naturalistic and evolutionary conceptualization with the neo-Kantian critique. A clearly delineated naturalistic and differential perspective of scientific research that brought about the birth of psychology as an experimental discipline in Italy in the 1900s emerges from the analysis, including psychology and psychopathology as studied by the phreniatrists Gabriele Buccola, Enrico Morselli, and Eugenio Tanzi; Tito Vignoli and Giuseppe Sergi's work in comparative anthropology; Giulio Fano's approach and contribution to physiology; and Enrico Ferri's contribution to criminology. (PsycINFO Database Record (c) 2012 APA, all rights reserved). PMID:23397915

  4. Networked alpha and gamma spectral acquisition and analysis system

    SciTech Connect

    Wilcox, C.M.; Gross, J.M.

    1993-10-01

    This manual assumes a knowledge of (terminology used and a working familiarity with) the windowing system and mouse of the Sun computer workstation. See the appropriate Sun manuals for additional information. ALDO, the alpha detector control program, is used to control, monitor, and edit log information associated with the collection of alpha spectra. Actual data collection and control functions are performed by Mizar Real-Time computers for which ALDO acts as a friendly user command interface and status display. It is normally started as part of your login procedure, but may also be started from the ``NETSPEC Utilities`` submenu of the root menu. The root menu is obtained by pushing the right mouse button when the cursor is over the root window (background picture). To become a user of ALDO and the other programs in the NETSPEC system, contact the person who performs systems administration tasks for the Sun computers. Most user interaction with ALDO is by means of mouse manipulation of screen items such as buttons, checkboxes, and sliders. The action of pushing the left mouse button when the cursor is over an item is called selecting that item. The left mouse button is therefore called the select button. The right mouse button is the menu button because a limited number of options may be displayed when that button is pressed when the cursor is over an item with a triangle (inverted delta). In this document, names of selectable items are printed in bold when they are first mentioned or when emphasis is helpful. In general, items which do not apply to the current context are either disabled or made invisible in order to prevent selection.

  5. Gender differences in endocrine responses to posture and 7 days of -6 degrees head-down bed rest

    NASA Technical Reports Server (NTRS)

    Vernikos, J.; Dallman, M. F.; Keil, L. C.; O'Hara, D.; Convertino, V. A.

    1993-01-01

    Endocrine regulation of fluids and electrolytes during 7 days of -6 degrees head-down bed rest (HDBR) was compared in male (n = 8) and, for the first time, female (n = 8) volunteers. The subjects' responses to quiet standing for 2 h before and after HDBR were also tested. In both sexes, diuresis and natriuresis were evident during the first 2-3 days of HDBR, resulting in a marked increase in the urinary Na(+)-to-K+ ratio and significant Na+ retention on re-ambulation. After the 1st day of HDBR, plasma renin activity (PRA) was increased relative to aldosterone (Aldo), plasma volume was decreased, and the renal response to Aldo appeared to be appropriate. Circulating levels of arginine vasopressin, cortisol, and ACTH were unchanged during HDBR. Plasma testosterone decreased slightly on day 2 of HDBR in males. The ratio of early morning ACTH to cortisol was lower in females than in males because ACTH was lower in females. Urinary cortisol increased and remained elevated throughout the HDBR in males only. There were no gender differences in the responses to 7 days of HDBR, except those in the pituitary-adrenal system; those differences appeared unrelated to the postural change. The provocative cardiovascular test of quiet standing before and after HDBR revealed both sex differences and effects of HDBR. There were significant sex differences in cardiovascular responses to standing before and after HDBR. Females had greater PRA and Aldo responses to standing before HDBR and larger Aldo responses to standing after HDBR than males.(ABSTRACT TRUNCATED AT 250 WORDS).

  6. FOREWORD: TAUP 2005: Proceedings of the Ninth International Conference on Topics in Astroparticle and Underground Physics

    NASA Astrophysics Data System (ADS)

    Bottino, Alessandro; Coccia, Eugenio; Morales, Julio; Puimedónv, Jorge

    2006-04-01

    The ninth meeting of the TAUP Workshop Series, TAUP 2005, was organized by the University of Zaragoza and Laboratorio Subterráneo de Canfranc, jointly with the Laboratori Nazionali del Gran Sasso of the Italian Institute of Nuclear Physics (INFN). It was dedicated to the memory of professor Angel Morales, co-founder of the TAUP Series and a central figure in the scientific shaping and organization of the TAUP conferences since their inception in 1989. He and his group of collaborators laid, twenty years ago, the foundations of underground physics in Spain. To have TAUP 2005 hosted by the University of Zaragoza was a tangible way of honouring his memory. The Conference was concluded by a visit to the new installations of the Canfranc Laboratory, where a memorial ceremony was held in honour of Angel Morales, the driving force for the creation of that Laboratory. In TAUP 2005 all the various aspects of Astroparticle Physics have been covered, from Cosmology and Dark Constituents, to Gravitational Waves, to Neutrino Physics and Astrophysics, to High Energy Astrophysics, to Cosmic Rays and Gamma-Rays Astronomy. New and important scientific results were presented and debated in the plenary review talks and in a very large number of contributions in topical parallel sessions. As editors of these proceedings, we hope that this volume, which contains most of the talks and contributions presented at TAUP 2005, will provide a detailed state-of-the-art account of the various facets of Astroparticle Physics. We thank all the invited speakers and contributors who made this possible. Full coverage of the transparencies presented at the conference can be found on the website http://www.unizar.es/taup2005. At TAUP 2005 a memorial lecture was delivered by Art McDonald to commemorate John Bahcall, who passed away prematurely in August 2005. In this talk, his figure, as a pioneer and leader in the fields of Neutrino Physics, Astronomy and Astrophysics and as a man of great personal

  7. Effect of paricalcitol on renin and albuminuria in non-diabetic stage III-IV chronic kidney disease: a randomized placebo-controlled trial

    PubMed Central

    2013-01-01

    Background Vitamin D receptor activators reduce albuminuria, and may improve survival in chronic kidney disease (CKD). Animal studies suggest that these pleiotropic effects of vitamin D may be mediated by suppression of renin. However, randomized trials in humans have yet to establish this relationship. Methods In a randomized, placebo-controlled, double-blinded crossover study, the effect of oral paricalcitol (2 μg/day) was investigated in 26 patients with non-diabetic, albuminuric stage III-IV CKD. After treatment, plasma concentrations of renin (PRC), angiotensin II (AngII) and aldosterone (Aldo) were measured. GFR was determined by 51Cr-EDTA clearance. Assessment of renal NO dependency was performed by infusion of NG-monomethyl-L-arginine (L-NMMA). Albumin excretion rate (AER) was analyzed in 24-h urine and during 51Cr-EDTA clearance. Results Paricalcitol did not alter plasma levels of renin, AngII, Aldo, or urinary excretion of sodium and potassium. A modest reduction of borderline significance was observed in AER, and paricalcitol abrogated the albuminuric response to L-NMMA. Conclusions In this randomized, placebo-controlled trial paricalcitol only marginally decreased AER and did not alter circulating levels of renin, AngII or Aldo. The abrogation of the rise in albumin excretion by paricalcitol during NOS blockade may indicate that favourable modulation of renal NO dependency could be involved in mediating reno-protection and survival benefits in CKD. Trial registration ClinicalTrials.gov identifier: NCT01136564 PMID:23889806

  8. A novel method for direct investigation of dark matter

    NASA Astrophysics Data System (ADS)

    Bertoni, R.; Chignoli, F.; Chiesa, D.; Clemenza, M.; Ghezzi, A.; Lucchini, G.; Mazza, R.; Negri, P.; Pozzi, S.; Pullia, A.; Redaelli, N.; Zanotti, L.; Cundy, D.

    2014-07-01

    The Materia OSCura A Bolle (MOSCAB) experiment uses a new technique for Dark Matter search. The Geyser technique is applied to the construction of a prototype detector with a mass of 0.5 kg and the encouraging results are reported here; an accent is placed on a big detector of 40 kg in construction at the Milano Bicocca University and INFN.

  9. Researchers warn of 'worrying imprudence' over cuts

    NASA Astrophysics Data System (ADS)

    Cartlidge, Edwin

    2012-08-01

    Italian physicists reacted with anger to proposed cuts to the country's research funding. Particularly hard hit would be the National Institute of Nuclear Physics (INFN), which has been slated to absorb nearly half of the €120m reduction in the research ministry's budget as outlined in a decree on 6 July.

  10. Windows beyond the Standard Model

    SciTech Connect

    Bernabei, R.

    2007-10-12

    DAMA is an observatory for rare processes at the Gran Sasso National Laboratory of the I.N.F.N. (LNGS). Here some arguments will be shortly summarised on the investigation on dark matter (DM) particles by annual modulation signature and on some of the performed searches for double beta decay modes.

  11. Section Editors

    NASA Astrophysics Data System (ADS)

    Groep, D. L.; Bonacorsi, D.

    2014-06-01

    1. Data Acquisition, Trigger and Controls Niko NeufeldCERNniko.neufeld@cern.ch Tassos BeliasDemokritosbelias@inp.demokritos.gr Andrew NormanFNALanorman@fnal.gov Vivian O'DellFNALodell@fnal.gov 2. Event Processing, Simulation and Analysis Rolf SeusterTRIUMFseuster@cern.ch Florian UhligGSIf.uhlig@gsi.de Lorenzo MonetaCERNLorenzo.Moneta@cern.ch Pete ElmerPrincetonpeter.elmer@cern.ch 3. Distributed Processing and Data Handling Nurcan OzturkU Texas Arlingtonnurcan@uta.edu Stefan RoiserCERNstefan.roiser@cern.ch Robert IllingworthFNAL Davide SalomoniINFN CNAFDavide.Salomoni@cnaf.infn.it Jeff TemplonNikheftemplon@nikhef.nl 4. Data Stores, Data Bases, and Storage Systems David LangeLLNLlange6@llnl.gov Wahid BhimjiU Edinburghwbhimji@staffmail.ed.ac.uk Dario BarberisGenovaDario.Barberis@cern.ch Patrick FuhrmannDESYpatrick.fuhrmann@desy.de Igor MandrichenkoFNALivm@fnal.gov Mark van de SandenSURF SARA sanden@sara.nl 5. Software Engineering, Parallelism & Multi-Core Solveig AlbrandLPSC/IN2P3solveig.albrand@lpsc.in2p3.fr Francesco GiacominiINFN CNAFfrancesco.giacomini@cnaf.infn.it Liz SextonFNALsexton@fnal.gov Benedikt HegnerCERNbenedikt.hegner@cern.ch Simon PattonLBNLSJPatton@lbl.gov Jim KowalkowskiFNAL jbk@fnal.gov 6. Facilities, Infrastructures, Networking and Collaborative Tools Maria GironeCERNMaria.Girone@cern.ch Ian CollierSTFC RALian.collier@stfc.ac.uk Burt HolzmanFNALburt@fnal.gov Brian Bockelman U Nebraskabbockelm@cse.unl.edu Alessandro de SalvoRoma 1Alessandro.DeSalvo@ROMA1.INFN.IT Helge MeinhardCERN Helge.Meinhard@cern.ch Ray PasetesFNAL rayp@fnal.gov Steven GoldfarbU Michigan Steven.Goldfarb@cern.ch

  12. PREFACE: XIV International Conference on Calorimetry in High Energy Physics

    NASA Astrophysics Data System (ADS)

    Wang, Yifang

    2011-03-01

    The International Conferences on Calorimetry in High Energy Physics (also known as the Calor Conference series, started in October 1990 at Fermilab) address all aspects of calorimetric particle detection and measurement, with an emphasis on high energy physics experiments. The XIV International Conference on Calorimetry in High Energy Physics (Calor 2010) was held at the campus of the Institute of High Energy Physics, Beijing, China, from May 10-14, 2010. This conference brought together more than 110 participants from 20 countries, including senior scientists and young physicists. During the five days of the conference, 98 presentations were given in seven plenary sessions. The attendees had in-depth discussions on the latest developments and innovations in calorimetry, including the exciting new LHC results. From the presentations, 83 papers were published in this proceedings. The success of the conference was due to the participants' enthusiasm and the excellent talks given by the speakers, and to the conveners for organizing the individual sessions. We would like to thank the International Advisory Committee for giving us the opportunity to host this Conference in Beijing. Finally we would like to thank all the people involved in the organization of the Conference, who have provided valuable local support. Yifang WangChair of Local Organizing Committee International Advisory Committee M DanilovITEP Moscow M DiemozINFN Roma I A EreditatoBern F L FabbriINFN Frascati T KobayashiICEPP Tokyo M LivanPavia University & INFN P LubranoINFN Perugia S MagillANL Argonne A MaioLIPP Lisbon H OberlackMPI Munich A ParaFermilab R WigmansTTU Lubbock R YoshidaANL Argonne R ZhuCaltech Local Organizing Committee Y WangIHEP (Chair) Y GaoTshinghua University T HuIHEP (Scientific secretary) C LiUSTC W LiIHEP J LuIHEP P WangIHEP T XuIHEP L ZhouIHEP Session Conveners 1) Materials and detectors - Junguang Lu (IHEP), Francesca Nessi (CERN) 2) Algorithm and simulation - Nural Akchurin

  13. PREFACE: Tenth International Conference on Topics in Astroparticle and Underground Physics (TAUP2007)

    NASA Astrophysics Data System (ADS)

    Inoue, Kunio; Suzuki, Atsuto; Mitsui, Tadao

    2008-07-01

    Otsuka, and Ms Yuri Endo, our workshop secretaries, for their continuous and excellent work in the organization of the conference, and to Ms Chiyo Itoh, and Ms Machiko Mizutani, for their invaluable assistance during the conference. We also gratefully thank the technical staff: Tomoaki Takayama, Hiromitsu Hanada, Takashi Nakajima, for their invaluable help. As announced at the end of the conference, TAUP 2009 will be held in Gran Sasso, Italy, hosted by the Istituto Nazionale di Fisica Nucleare (INFN) with the chair of Professor Eugenio Coccia. Kunio Inoue, Atsuto Suzuki and Tadao Mitsui COMMITTEES TAUP Steering Committee F T Avignone U South Carolina B C Barish CALTECH E Bellotti U Milano/INFN J Bernabéu U Valencia A Bottino (chair) U Torino/INFN V de Alfaro U Torino/INFN T Kajita ICRR U Tokyo C W Kim Johns Hopkins Univ /KIAS E Lorenz U München V Matveev INR Moskow J Morales U Zaragoza D Sinclair U Carleton M Spiro IN2P3 TAUP 2007 International Advisory Committee J J Aubert CNRS Marseille M Baldo-Ceolin U Padova/INFN V Berezinsky INFN-LNGS/INR L Bergström U Stockholm R Bernabei U Roma Tor Vergata/INFN A Bettini U Padova/INFN S Bilenky JINR Dubna D O Caldwell U C Santa Barbara E Coccia INFN-LNGS/U Roma Tor Vergata J Cronin U Chicago A Dar Technion Haifa G Domogatsky INR Moscow H Ejiri U Osaka J Ellis CERN E Fernández IFAE Barcelona E Fiorini U Milano/INFN G Fogli U Bari/INFN T Gaisser U Delaware G Gelmini UCLA G Gerbier CEA Saclay F Halzen U Wisconsin W Haxton U Washington T Kirsten MPI Heidelberg L Maiani U Roma/INFN A McDonald Queen's U K Nakamura KEK E Peterson U Minneapolis R Petronzio INFN/U Roma Tor Vergata G Raffelt MPI München R Rebolo IAC Tenerife L Resvanis U Athens P Salati U Savoie/LAPTH Annecy A Smirnov ICTP Trieste N Spooner U Sheffield S Ting MIT/CERN Y Totsuka U Tokyo M S Turner FNAL/U Chicago J W F Valle IFIC Valencia D Vignaud APC Paris F von Feilitzsch T U München G Zatsepin INR Moscow TAUP 2007 Organizing Committee A Bottino U Torino/INFN D

  14. Mineralocorticoid and angiotensin II type 1 receptors in the subfornical organ mediate angiotensin II - induced hypothalamic reactive oxygen species and hypertension.

    PubMed

    Wang, Hong-Wei; Huang, Bing S; White, Roselyn A; Chen, Aidong; Ahmad, Monir; Leenen, Frans H H

    2016-08-01

    Activation of angiotensinergic pathways by central aldosterone (Aldo)-mineralocorticoid receptor (MR) pathway plays a critical role in angiotensin II (Ang II)-induced hypertension. The subfornical organ (SFO) contains both MR and angiotensin II type 1 receptors (AT1R) and can relay the signals of circulating Ang II to downstream nuclei such as the paraventricular nucleus (PVN), supraoptic nucleus (SON) and rostral ventrolateral medulla (RVLM). In Wistar rats, subcutaneous (sc) infusion of Ang II at 500ng/min/kg for 1 or 2weeks increased reactive oxygen species (ROS) as measured by dihydroethidium (DHE) staining in a nucleus - specific pattern. Intra-SFO infusion of AAV-MR- or AT1aR-siRNA prevented the Ang II-induced increase in AT1R mRNA expression in the SFO and decreased MR mRNA. Both MR- and AT1aR-siRNA prevented increases in ROS in the PVN and RVLM. MR- but not AT1aR-siRNA in the SFO prevented the Ang II-induced ROS in the SON. Both MR- and AT1aR-siRNA in the SFO prevented most of the Ang II-induced hypertension as assessed by telemetry. These results indicate that Aldo-MR signaling in the SFO is needed for the activation of Ang II-AT1R-ROS signaling from the SFO to the PVN and RVLM. Activation of Aldo-MR signaling from the SFO to the SON may enhance AT1R dependent activation of pre-sympathetic neurons in the PVN. PMID:27163380

  15. Rapid, dynamic changes in glomerular permeability to macromolecules during systemic angiotensin II (ANG II) infusion in rats.

    PubMed

    Axelsson, Josefin; Rippe, Anna; Oberg, Carl M; Rippe, Bengt

    2012-09-15

    The actions of systemic angiotensin II (ANG II) infusions on glomerular permeability were investigated in vivo. In anesthetized Wistar rats (250-280 g), the left ureter was cannulated for urine collection, while simultaneously blood access was achieved. Rats were continuously infused intravenously with either of four doses of ANG II ranging from 16 ng·kg(-1)·min(-1) (Lo-ANG II) to 1.82 μg·kg(-1)·min(-1) (Hi-ANG II), and in separate experiments with aldosterone (Aldo; 0.22 mg·kg(-1)·min(-1)), or with the calcium channel blocker nimodipine, or with the Aldo antagonist spironolactone together with a high ANG II dose (910 ng·kg(-1)·min(-1); Hi-Int-ANG II), respectively, and with polydisperse FITC-Ficoll-70/400 (molecular radius 10-80 Å) and (51)Cr-EDTA. Plasma and urine samples were taken at 5, 15, 30, 60, and 120 min and analyzed by high performance size-exclusion chromatography for determination of glomerular sieving coefficients (θ) to Ficoll. Mean arterial pressure (MAP) and glomerular filtration rate (GFR) were also assessed. For ANG II, there was a rapid, marked, partly reversible increase in glomerular permeability (θ) for Ficoll molecules >34 Å in radius, peaking at 5-15 min, which was completely abrogated by the ANG II blocker candesartan but not affected by spironolactone at 15 and 30 min. For Aldo, the response was similar to that found for the lowest dose of ANG II infused. For the two highest ANG II doses given (Hi-Int-ANG II and Hi-ANG II), GFR decreased transiently, concomitant with marked, sustained increases in MAP. Nimodipine completely blocked all hemodynamic ANG II actions, whereas the glomerular permeability response remained unchanged. Thus ANG II directly increased glomerular permeability independently of its hemodynamic actions and largely independently of the concomitant Aldo response. The ANG II-induced increases in glomerular permeability were, according to a two-pore and a log-normal distributed pore model, compatible with an

  16. The pigmented portio: benign lentigo of the uterine cervix.

    PubMed

    Schneider, V; Zimberg, S T; Kay, S

    1981-01-01

    The first case of benign lentigo of the uterine cervix is reported, providing further evidence that the cervix is able to form the entire spectrum of melanocytic lesions known to occur in the skin. Review of the literature reveals 19 cases of primary malignant melanoma of the cervix and 30 cases of blue nevus. The reported case offers support that benign lentigines may play a role as premalignant lesions of malignant melanoma not only in the skin, but aldo in mucous membranes. PMID:7327080

  17. The dawn of open heart surgery: an overview of the symposium that celebrated the 50th anniversary of controlled cross circulation.

    PubMed

    DeWall, Richard

    2005-01-01

    At the 50th Anniversary of Open Heart Surgery symposium sponsored by the Lillehei Heart Institute of the University of Minnesota in October 2004, the following pioneers in open heart surgery development presented papers of historical interest: Drs. Peter Agre, Robert W. Anderson, William Baumgartner, Alain Carpentier, Aldo Casteneda, Randolph Chitwood, Jr., Denton Cooley, Fred Crawford, Michael DeBakey, Richard DeWall, Vincent Gott, Claude Lenfant, Floyd Loop, James Holler, Glen Nelson, Norman Shumway, Manny Villafana, Richard Weisel, and Sir Magdi Yacoub. PMID:16106145

  18. X-38 V-132 Free Flight 2 (This is a video tape)

    NASA Technical Reports Server (NTRS)

    Bordano, Aldo J.

    2000-01-01

    Mr. Aldo Bordano will be presenting details of some of the JSC flight mechanics involvement in the X-38 testing program. Focus shall be on the parafoil system with regards its testing, performance analysis, and GN&C. An excellent example of a recent flight test at Dryden Flight Research Center shall be shown which portrays the system characteristics, sequencing, performance, and testing techniques. The intent is to inform the scientific and engineering communities about the developments in the X-38 parafoil program, as well as invite feedback on potential improvements in testing or systems.

  19. The X-Ray Spectra of Blazars: Analysis of the Complete EXOSAT Archive: Erratum

    NASA Astrophysics Data System (ADS)

    Sambruna, Rita M.; Barr, Paul; Giommi, Paolo; Maraschi, Laura; Tagliaferri, Gianpiero; Treves, Aldo

    1995-07-01

    In the paper "The X-Ray Spectra of Blazars: Analysis of the Complete EXOSAT Archive" by Rita M. Sambruna, Paul Barr, Paolo Giommi, Laura Maraschi, Gianpiero Tagliaferri, and Aldo Treves (ApJS, 95,371 [1994]), the section regarding the object PKS 1510-08 (Section 4.4.14) contains an erroneous quotation. K. P. Singh, A.R. Rao, and M.N. Vahia (ApJ, 365,455 [1990]) in fact detected: emission line only in the 1984 data, and not in the 1985 spectrum, as stated.

  20. Androgen-metabolizing enzymes: A structural perspective.

    PubMed

    Manenda, Mahder Seifu; Hamel, Charles Jérémie; Masselot-Joubert, Loreleï; Picard, Marie-Ève; Shi, Rong

    2016-07-01

    Androgen-metabolizing enzymes convert cholesterol, a relatively inert molecule, into some of the most potent chemical messengers in vertebrates. This conversion involves thermodynamically challenging reactions catalyzed by P450 enzymes and redox reactions catalyzed by Aldo-Keto Reductases (AKRs). This review covers the structures of these enzymes with a focus on active site interactions and proposed mechanisms. Due to their role in a number of diseases, particularly in cancer, androgen-metabolizing enzymes have been targets of drug design. Hence we will also highlight how existing knowledge of structure is being used to this end. PMID:26924584

  1. Identification of Lactobacillus brevis using a species-specific AFLP-derived marker.

    PubMed

    Fusco, Vincenzina; Quero, Grazia Marina; Chieffi, Daniele; Franz, Charles M A P

    2016-09-01

    A simple and specific method for the rapid detection and identification of Lactobacillus brevis was developed. A fAFLP (Fluorescent Amplified Fragment Length Polymorphisms) marker for L. brevis was used to design oligonucleotide primers for a species-specific PCR assay, targeting a 125bp fragment of the gene encoding the aldo/keto reductase of the diketogulonate-reductase family of L. brevis. This assay resulted in 100% inclusivity and exclusivity of assignment of strains to the species L. brevis. The analytical specificity of this assay was successfully tested to identify L. brevis isolates from sourdoughs. PMID:27289191

  2. Leopold Fellows meet

    NASA Astrophysics Data System (ADS)

    Hartmann, Dennis L.

    Communicating the results of environmental science research to nonscientists was the focus of a week-long meeting of fellows of the Aldo Leopold Leadership Program (ALLP) in June. Participating were 20 mid-career university facultymembers, the first group of ALLP fellows to be selected.Discussions were held on the role of scientists in society, techniques for effective leadership, improving communications skills, and working productively with news media. The training program considers both print and broadcast media as well as other outreach vehicles. The meeting was held at Kah Nee Ta Resort on the Warm Springs Indian Reservation, Warm Springs, Oregon, June 15-21, 1999.

  3. Self and other in communication and cognition: the role of auto-communication and intersubjectivity in autopoiesis of psychic systems.

    PubMed

    Märtsin, Mariann

    2008-06-01

    By referring to Niklas Luhmann's theory of self-referential systems, Aldo Mascareño (2008, submitted for publication) gives an account of system-environment interrelatedness, explaining how social and individual constitute each other through the process of communication and co-creation of meanings. Two possible extensions to his account are discussed. Firstly, auto-communication within the system that happens without any external reference needs to be taken into account while describing the existence and constant re-creation of psychic systems. Secondly, in order for the system and environment or two systems to communicate, an imagined and temporary intersubjectivity between the two needs to be assumed. PMID:18516657

  4. Determination of the half-life of 213Fr with high precision

    NASA Astrophysics Data System (ADS)

    Fisichella, M.; Musumarra, A.; Farinon, F.; Nociforo, C.; Del Zoppo, A.; Figuera, P.; La Cognata, M.; Pellegriti, M. G.; Scuderi, V.; Torresi, D.; Strano, E.

    2013-07-01

    High-precision measurement of half-life and Qα value of neutral and highly charged α emitters is a major subject of investigation currently. In this framework, we recently pushed half-life measurements of neutral emitters to a precision of a few per mil. This result was achieved by using different techniques and apparatuses at Istituto Nazionale di Fisica Nucleare Laboratori Nazionali del Sud (INFN-LNS) and GSI Darmstadt. Here we report on 213Fr half-life determination [T1/2(213Fr) = 34.14±0.06 s] at INFN-LNS, detailing the measurement protocol used. Direct comparison with the accepted value in the literature shows a discrepancy of more than three sigma. We propose this new value as a reference, discussing previous experiments.

  5. COMMITTEES: Proceedings of the 13th Gravitational Waves Data Analysis Workshop (GWDAW13), San Juan, Puerto Rico, 19-22 January 2009 Proceedings of the 13th Gravitational Waves Data Analysis Workshop (GWDAW13), San Juan, Puerto Rico, 19-22 January 2009

    NASA Astrophysics Data System (ADS)

    2009-10-01

    Science Organising Committee (SOC) Bruce Allen, AEI, Germany Patrick Brady, University of Wisconsin Milwaukee, USA Deepto Chakrabarty, MIT, USA Eugenio Coccia, INFN, Gran Sasso, Italy James Cordes, Cornell University, USA Mario Díaz (Chair), University of Texas Brownsville, USA Sam Finn, Penn State, USA Neil Gehrels, NASA GSFC, USA Fredrick A Jenet, University of Texas Brownsville, USA Nobuyuki Kanda, Osaka City University, Japan Erik Katsavounides, MIT, USA Dick Manchester, ATNF, Australia Soumya Mohanty, University of Texas Brownsville, USA Benoit Mours, LAPP-Annecy, France Maria Alessandra Papa, AEI, Germany Kate Scholberg, Duke University, USA Susan Scott, The Australian National University Alberto Vecchio, University of Birmingham, UK Andrea Vicere, INFN - Sezione di Firenze, Italy Stan Whitcomb, LIGO CALTECH, USA Local Organising Committee (LOC) Paulo Freire (Arecibo Observatory, Puerto Rico) Murray Lewis (Arecibo Observatory, Puerto Rico) Wanda Wiley (University of Texas Brownsville, USA)

  6. Status of the SPARC Project

    SciTech Connect

    Alesini, D.; Bertolucci, S.; Bellaveglia, M.; Biagini, M.E.; Boni, R.; Boscolo, M.; Castellano, M.; Clozza, A.; Pirro, G.Di; Drago, A.; Esposito, A.; Ferrario, M.; Ficcadenti, L.; Filippetto, D.; Fusco, V.; Gallo, A.; Gatti, G.; Ghigo, A.; Guiducci, S.; Incurvati, M.; Ligi, C.; /Frascati /INFN, Milan /Milan U. /INFN, Rome /INFN, Rome /ENEA, Frascati /Milan, Polytechnic /UCLA /SLAC

    2006-01-25

    The SPARC project has entered its installation phase at the Frascati National Laboratories of INFN: its main goal, the promotion of an R&D activity oriented to the development of a high brightness photoinjector to drive SASE-FEL experiments, is being vigorously pursued by a collaboration among ENEA-INFN-CNR-Universita di Roma Tor Vergata-INFM-ST. In this paper we will report on the installation and test of some major components, like Ti:Sa laser system, RF gun and RF power system. Advancements in the control and beam diagnostics systems will also be reported, in particular on the emittance-meter device for beam emittance measurements in the drift space downstream the RF gun. Recent results on laser pulse shaping show the feasibility of producing 10 ps flat-top laser pulses in the UV with rise time below 1 ps. First FEL experiments have been proposed, using SASE, seeding and non-linear resonant harmonics.

  7. NUMEN Project @ LNS : Heavy Ions Double Charge Exchange as a tool towards the 0νββ Nuclear Matrix Element

    NASA Astrophysics Data System (ADS)

    Agodi, C.; Cappuzzello, F.; Bonanno, D. L.; Bongiovanni, D. G.; Branchina, V.; Calabrese, S.; Calabretta, L.; Calanna, A.; Carbone, D.; Cavallaro, M.; Colonna, M.; Foti, A.; Finocchiaro, P.; Greco, V.; Lanzalone, G.; Lo Presti, D.; Longhitano, F.; Muoio, A.; Pandola, L.; Rifuggiato, D.; Tudisco, S.

    2016-06-01

    The NUMEN Project, proposed at INFN Laboratori Nazionali del Sud (LNS) in Catania, has the aim to access the nuclear matrix elements, entering the expression of the life time of double beta decay, by relevant cross sections of double charge exchange reactions. The basic point, on which it is based this innovative technique, is the coincidence of the initial and final state wave-functions in the two classes of processes and the similarity of the transition operators. A key aspect of the Project is the use of MAGNEX large acceptance magnetic spectrometer, for the detection of the ejectiles, and of the INFN LNS K800 Superconducting Cyclotron (CS), for the acceleration of the required high resolution and low emittance heavy-ion beams.

  8. On-line remote monitoring of radioactive waste repositories

    NASA Astrophysics Data System (ADS)

    Calì, Claudio; Cosentino, Luigi; Litrico, Pietro; Pappalardo, Alfio; Scirè, Carlotta; Scirè, Sergio; Vecchio, Gianfranco; Finocchiaro, Paolo; Alfieri, Severino; Mariani, Annamaria

    2014-12-01

    A low-cost array of modular sensors for online monitoring of radioactive waste was developed at INFN-LNS. We implemented a new kind of gamma counter, based on Silicon PhotoMultipliers and scintillating fibers, that behaves like a cheap scintillating Geiger-Muller counter. It can be placed in shape of a fine grid around each single waste drum in a repository. Front-end electronics and an FPGA-based counting system were developed to handle the field data, also implementing data transmission, a graphical user interface and a data storage system. A test of four sensors in a real radwaste storage site was performed with promising results. Following the tests an agreement was signed between INFN and Sogin for the joint development and installation of a prototype DMNR (Detector Mesh for Nuclear Repository) system inside the Garigliano radwaste repository in Sessa Aurunca (CE, Italy). Such a development is currently under way, with the installation foreseen within 2014.

  9. Study of silicon photomultipliers fast amplifier and thermoregulation

    NASA Astrophysics Data System (ADS)

    D'Antone, I.; Fabbri, L.; Foschi, E.; Guandalini, C.; Laurenti, G.; Lax, I.; Levi, G.; Quadrani, L.; Sbarra, Ca.; Sbarra, Cr.; Villa, M.; Zoccoli, A.; Zuffa, M.

    2011-02-01

    The silicon photomultipliers (SiPM) are adopted in various physical applications, from medical physics to astrophysics, for their advantages in terms of cost and weight with respect to traditional photo detectors. Their low bias voltage supply (about 30 V), hardiness and resistance to magnetic field are ideal characteristics for space application. In the frame of INFN-Irst collaboration, some of them have been developed and produced at FBK (Trento-Italy), and have been characterized in the INFN laboratories of Bologna (DaSiPM2 collaboration). The SiPM can be used in conjunction with fibres and counters in high energy physics experiments. To exploit the SiPM time resolution, a fast amplifier has been studied. The SiPM gain depends critically on temperature and a thermal stabilization is also necessary. The use of a thermoelectric cooler module based on a Peltier cell has been investigated, and the results are shown.

  10. PREFACE: Young Researcher Meeting in Rome 2012

    NASA Astrophysics Data System (ADS)

    Agostini, Fabio; Cattani, Giordano; Mazzaferro, Luca; Migliaccio, Marina; Pietrobon, Davide; Ricci Pacifici, Daniel; Stellato, Francesco; Veneziani, Marcella

    2012-10-01

    Conference logo At its third edition, the Young Researcher Meeting in Rome (YRMR) proves to be a growing event in the Italian scientific panorama. The high-quality content of the abstracts submitted to the scientific committee resulted in an exciting conference, held, for the second time, at the University of Rome 'Tor Vergata' on 20 January 2012. A busy schedule covered a large variety of cutting-edge science topics: fundamental interactions, particle physics, cosmology, astrophysics, condensed matter and biomedical physics. The broad range of the subjects discussed is the distinctive feature of the YRMR, a meeting aimed at enhancing the synergy among complementary branches of science by stimulating a fruitful exchange between theoretical, experimental and computational physics. Promoting collaborations between PhD students, postdoctoral fellows and young researchers creates a solid scientific network with an open-minded approach to discovery. In this volume, we collect the contributions that have been presented both in the form of talks and of posters. YRMR Organising and Editorial Committee Fabio Agostini (fabio.agostini@roma2.infn.it) Dipartimento di Fisica, Università di Roma 'Tor Vergata' Via della Ricerca Scientifica 1, 00133 Roma Italy Giordano Cattani (giordano.cattani@roma2.infn.it) Dipartimento di Fisica, Università di Roma 'Tor Vergata' INFN sezione di Roma 'Tor Vergata' Via della Ricerca Scientifica 1, 00133 Roma Italy Luca Mazzaferro (luca.mazzaferro@roma2.infn.it) Dipartimento di Fisica, Università di Roma 'Tor Vergata' INFN sezione di Roma 'Tor Vergata' Via della Ricerca Scientifica 1, 00133 Roma Italy Marina Migliaccio (migliaccio@ifca.unican.es) Instituto de Fisica de Cantabria, Edificio Juan Jorda, Avenida de los Castros, E-39005 Santander, Cantabria Spain Davide Pietrobon (davide.pietrobon@jpl.nasa.gov) Jet Propulsion Laboratory - California Institute of Technology 4800 Oak Grove Drive 169-237 91109 Pasadena, CA USA Daniel Ricci Pacifici

  11. RIB Production at LNL: the EXOTIC Facility

    NASA Astrophysics Data System (ADS)

    Marco, Mazzocco

    2016-04-01

    Nuclear reactions involving radioactive isotopes are extremely relevant in several astrophysical scenarios, from the Big-Bang Nucleosynthesis to Supernovae explosions. In this contribution the production of Radioactive Ion Beams (RIBs) by means of the in-flight technique is reviewed. In particular, the use of direct reactions in inverse kinematics for the production of light weakly-bound RIBs by means of the facility EXOTIC at INFN-LNL (Italy) will be described in detail.

  12. Modern Detectors for Astroparticle Physics

    SciTech Connect

    Adriani, Oscar

    2005-10-12

    This paper focus on the necessary requirements for a modern astroparticle physics detector based either on stratospheric balloons, either on satellite. The main technical solutions used to build a reliable detector are described. Finally, the most relevant experiments that have been developed with the INFN contribution and that will be ready in the near future (both for {gamma} and charged cosmic rays detection) are described.

  13. PArthENoPE: Public Algorithm Evaluating the Nucleosynthesis of Primordial Elements

    SciTech Connect

    Pisanti, O.; Cirillo, A.; Esposito, S.; Iocco, F.; Mangano, G.; Miele, G.; Serpico, P.D.

    2007-05-04

    We describe a program for computing the abundances of light elements produced during Big Bang Nucleosynthesis which is publicly available at http://parthenope.na.infn.it/. Starting from nuclear statistical equilibrium conditions the program solves the set of coupled ordinary differential equations, follows the departure from chemical equilibrium of nuclear species, and determines their asymptotic abundances as function of several input cosmological parameters as the baryon density, the number of effective neutrino, the value of cosmological constant and the neutrino chemical potential.

  14. Status and perspective of the GERDA neutrinoless double beta decay experiment

    NASA Astrophysics Data System (ADS)

    Knöpfle, K. T.; Gerda Collaboration

    2012-09-01

    Gerda, the GERManium Detector Array [1], is a new double beta decay experiment which is currently under commissioning in the Infn National Gran Sasso Laboratory (Lngs), Italy. It is implementing a new shielding concept by operating bare Ge diodes - enriched in 76Ge - in high purity liquid argon supplemented by a water shield. The paper presents the status of the experiment, results from the commissioning, and a summary of planned future activities.

  15. Explodet Project:. Methods of Automatic Data Processing and Analysis for the Detection of Hidden Explosive

    NASA Astrophysics Data System (ADS)

    Lecca, Paola

    2003-12-01

    The research of the INFN Gruppo Collegato di Trento in the ambit of EXPLODET project for the humanitarian demining, is devoted to the development of a software procedure for the automatization of data analysis and decision taking about the presence of hidden explosive. Innovative algorithms of likely background calculation, a system based on neural networks for energy calibration and simple statistical methods for the qualitative consistency check of the signals are the main parts of the software performing the automatic data elaboration.

  16. Nuclear Physics for Compact Stars

    SciTech Connect

    Baldo, M.

    2009-05-04

    A brief overview is given of the different lines of research developed under the INFN project 'Compact Stellar Objects and Dense Hadronic Matter' (acronym CT51). The emphasis of the project is on the structure of Neutron Stars (NS) and related objects. Starting from crust, the different Nuclear Physics problems are described which are encountered going inside a NS down to its inner core. The theoretical challenges and the observational inputs are discussed in some detail.

  17. Recent Results of Explorer and NAUTILUS

    NASA Astrophysics Data System (ADS)

    Fafone, V.; Rog Collaboration

    2002-12-01

    The most recent results of the resonant-mass gravitational wave (GW) detectors NAUTILUS and EXPLORER, operated by the ROG collaboration at the INFN Frascati National Laboratories and at CERN respectively, are reported. The passage of cosmic rays has been observed to excite mechanical vibrations in NAUTILUS. The new read-out electronics and the improved sensitivity of EXPLORER-with a bandwidth larger than 40 Hz at the level of 5·10-21 Hz-1/2 - are described.

  18. A new active thermal neutron detector.

    PubMed

    Bedogni, R; Bortot, D; Pola, A; Introini, M V; Gentile, A; Esposito, A; Gómez-Ros, J M; Palomba, M; Grossi, A

    2014-10-01

    This communication presents the main results about the design and in-house fabrication of a new solid-state neutron detector, which produces a DC output signal proportional to the thermal neutron fluence rate. The detector has been developed within the framework of the 3-y project NESCOFI@BTF of INFN (CSN V). Due to its sensitivity, photon rejection, low cost and minimum size, this device is suited to be used in moderator-based spectrometers. PMID:24345462

  19. Design and test of an extremely high resolution Timing Counter for the MEG II experiment: preliminary results

    NASA Astrophysics Data System (ADS)

    De Gerone, M.; Gatti, F.; Ootani, W.; Uchiyama, Y.; Nishimura, M.; Shirabe, S.; Cattaneo, P. W.; Rossella, M.

    2014-02-01

    The design and tests of Timing Counter elements for the upgrade of the MEG experiment, MEG II, are presented. The detector is based on several small plates of scintillator with a Silicon PhotoMultipliers dual-side readout. The optimisation of the single counter elements (SiPMs, scintillators, geometry) is described. Moreover, the results obtained with a first prototype tested at the Beam Test Facility (BTF) of the INFN Laboratori Nazionali di Frascati (LNF) are presented.

  20. RF tests of the beta - 0.5 five cell TRASCO cavities

    SciTech Connect

    A. Bosotti; Carlo Pagani; P. Pierini; J.P. Charrier; B. Visentin; Gianluigi Ciovati; Peter Kneisel

    2004-07-01

    Two complete 5 cell superconducting cavities at {beta} = 0.5 have been fabricated in the TRASCO INFN program. The cavities have been designed to minimize peak electric and magnetic fields, with a goal of 8.5 MV/m of accelerating gradient, at a Q > 5 10{sup 9}. The cavities have been tested in vertical cryostats at TJNAF and Saclay and the results are summarized here.

  1. The AMY experiment: Microwave emission from air shower plasmas

    NASA Astrophysics Data System (ADS)

    Alvarez-Muñiz, J.; Blanco, M.; Boháčová, M.; Buonomo, B.; Cataldi, G.; Coluccia, M. R.; Creti, P.; De Mitri, I.; Di Giulio, C.; Facal San Luis, P.; Foggetta, L.; Gaïor, R.; Garcia-Fernandez, D.; Iarlori, M.; Le Coz, S.; Letessier-Selvon, A.; Louedec, K.; Maris, I. C.; Martello, D.; Mazzitelli, G.; Monasor, M.; Perrone, L.; Petrera, S.; Privitera, P.; Rizi, V.; Rodriguez Fernandez, G.; Salamida, F.; Salina, G.; Settimo, M.; Valente, P.; Vazquez, J. R.; Verzi, V.; Williams, C.

    2016-07-01

    You The Air Microwave Yield (AMY) experiment investigate the molecular bremsstrahlung radiation emitted in the GHz frequency range from an electron beam induced air-shower. The measurements have been performed at the Beam Test Facility (BTF) of Frascati INFN National Laboratories with a 510 MeV electron beam in a wide frequency range between 1 and 20 GHz. We present the apparatus and the results of the tests performed.

  2. A magneto-optical trap for radioactive atoms

    NASA Astrophysics Data System (ADS)

    Mariotti, E.; Khanbekyan, K.; Marinelli, C.; Marmugi, L.; Moi, L.; Corradi, L.; Dainelli, A.; Calabrese, R.; Mazzocca, G.; Tomassetti, L.; Minguzzi, P.

    2013-03-01

    This paper presents the recent results of the TrapRad/Francium collaboration whose final aim is the measurement of the Atomic Parity Non-Conservation effect (APNC) in Francium atoms stored in a Magneto - Optical Trap (MOT) built at the Laboratori Nazionali di Legnaro (LNL) of the National Institute for Nuclear Physics (INFN). Current developments and new strategies to enhance the trapping efficiency of Francium isotopes and to detect new spectroscopic features are reported.

  3. The SPARX Project: R & D Activity Towards X-Rays FEL Sources

    SciTech Connect

    Alesini, D.; Bellaveglia, M.; Bertolucci, S.; Biagini, M.E.; Boni, R.; Boscolo, M.; Castellano, M.; Clozza, A.; Di Pirro, G.; Drago, A.; Esposito, A.; Ferrario, M.; Filippetto, D.; Fusco, V.; Gallo, A.; Ghigo, A.; Guiducci, S.; Incurvati, M.; Ligi, C.; Marcellini, F.; Migliorati, M.; /Frascati /ENEA, Frascati /INFN, Milan /INFN, Rome /INFN, Rome2 /Milan Polytechnic /UCLA /SLAC

    2005-08-05

    SPARX is an evolutionary project proposed by a collaboration among ENEA-INFN-CNR-Universita di Roma Tor Vergata aiming at the construction of a FELSASE X-ray source in the Tor Vergata Campus. The first phase of the SPARX project, funded by Government Agencies, will be focused on R&D activity on critical components and techniques for future X-ray facilities as described in this paper.

  4. Test of a coaxial blade tuner at HTS FNAL

    SciTech Connect

    Pischalnikov, Y.; Barbanotti, S.; Harms, E.; Hocker, A.; Khabiboulline, T.; Schappert, W.; Bosotti, A.; Pagani, C.; Paparella, R.; /LASA, Segrate

    2011-03-01

    A coaxial blade tuner has been selected for the 1.3GHz SRF cavities of the Fermilab SRF Accelerator Test Facility. Results from tuner cold tests in the Fermilab Horizontal Test Stand are presented. Fermilab is constructing the SRF Accelerator Test Facility, a facility for accelerator physics research and development. This facility will contain a total of six cryomodules, each containing eight 1.3 GHz nine-cell elliptical cavities. Each cavity will be equipped with a Slim Blade Tuner designed by INFN Milan. The blade tuner incorporates both a stepper motor and piezo actuators to allow for both slow and fast cavity tuning. The stepper motor allows the cavity frequency to be statically tuned over a range of 500 kHz with an accuracy of several Hz. The piezos provide up to 2 kHz of dynamic tuning for compensation of Lorentz force detuning and variations in the He bath pressure. The first eight blade tuners were built at INFN Milan, but the remainder are being manufactured commercially following the INFN design. To date, more than 40 of the commercial tuners have been delivered.

  5. PREFACE: XIII International Conference on Calorimetry in High Energy Physics (CALOR 2008)

    NASA Astrophysics Data System (ADS)

    Livan, Michele

    2009-07-01

    presentations, now reported in these proceedings, that were debated in stimulating and fruitful discussions. Outside of the Workshop, the participants were able to visit the historical Halls and Museum of the University, whose foundation dates back to the year 1361, and to enjoy a visit to the Certosa, a Carthusian monastery renowned for its exuberant architecture. Pavia welcomed the Conference participants by opening the doors of the Town Hall and offering a reception during which the Mayor's address underlined the importance of research and its applications in modern society. The successful organization and the smooth running of the Conference is due to many people and Institutions. We gratefully acknowledge the financial support of the Istituto Nazionale di Fisica Nucleare (INFN), the Department of Nuclear and Theoretical Physics and the University of Pavia, that made the Workshop possible, together with the contribution of our sponsors. The University also opened some rooms of the Chancellor's suite for the lunch and coffee breaks, and hosted the Conference Secretariat. We would like to express our deepest gratitude to the INFN and Department technical staff, who helped to prepare the Hall for the Conference and to provide computer services, and to the staff of the Theresian Library, who gave us access to the Room and organized a display of the many historical books from their vast and precious collections which are of interest to physicists. Above all, the success of the meeting is due to the participants who animated it, and in particular to the speakers for their dedicated work in preparing their excellent talks and in providing the write-ups, and to the conveners for their essential role in shaping an interesting and well balanced scientific program. Finally, we wish to thank the International Advisory Committee for their unfailing support and for offering us the opportunity to organize this Conference in Pavia. Michele Livan Chairman, Organizing Committee International

  6. Virtual pools for interactive analysis and software development through an integrated Cloud environment

    NASA Astrophysics Data System (ADS)

    Grandi, C.; Italiano, A.; Salomoni, D.; Calabrese Melcarne, A. K.

    2011-12-01

    WNoDeS, an acronym for Worker Nodes on Demand Service, is software developed at CNAF-Tier1, the National Computing Centre of the Italian Institute for Nuclear Physics (INFN) located in Bologna. WNoDeS provides on demand, integrated access to both Grid and Cloud resources through virtualization technologies. Besides the traditional use of computing resources in batch mode, users need to have interactive and local access to a number of systems. WNoDeS can dynamically select these computers instantiating Virtual Machines, according to the requirements (computing, storage and network resources) of users through either the Open Cloud Computing Interface API, or through a web console. An interactive use is usually limited to activities in user space, i.e. where the machine configuration is not modified. In some other instances the activity concerns development and testing of services and thus implies the modification of the system configuration (and, therefore, root-access to the resource). The former use case is a simple extension of the WNoDeS approach, where the resource is provided in interactive mode. The latter implies saving the virtual image at the end of each user session so that it can be presented to the user at subsequent requests. This work describes how the LHC experiments at INFN-Bologna are testing and making use of these dynamically created ad-hoc machines via WNoDeS to support flexible, interactive analysis and software development at the INFN Tier-1 Computing Centre.

  7. The GINGERino ring laser gyroscope, seismological observations at one year from the first light

    NASA Astrophysics Data System (ADS)

    Simonelli, Andreino; Belfi, Jacopo; Beverini, Nicolò; Di Virgilio, Angela; Carelli, Giorgio; Maccioni, Enrico; De Luca, Gaetano; Saccorotti, Gilberto

    2016-04-01

    The GINGERino ring laser gyroscope (RLG) is a new large observatory-class RLG located in Gran Sasso underground laboratory (LNGS), one national laboratory of the INFN (Istituto Nazionale di Fisica Nucleare). The GINGERino apparatus funded by INFN in the context of a larger project of fundamental physics is intended as a pathfinder instrument to reach the high sensitivity needed to observe general relativity effects; more details are found at the URL (https://web2.infn.it/GINGER/index.php/it/). The sensitivity reached by our instrument in the first year after the set up permitted us to acquire important seismological data of ground rotations during the transit of seismic waves generated by seisms at different epicentral distances. RLGs are in fact the best sensors for capturing the rotational motions associated with the transit of seismic waves, thanks to the optical measurement principle, these instruments are in fact insensitive to translations. Ground translations are recorded by two seismometers: a Nanometrics Trillium 240 s and Guralp CMG 3T 360 s, the first instrument is part of the national earthquake monitoring program of the Istituto Nazionale di Geofisica e Vulcanologia (INGV) and provides the ground translation data to be compared to the RLG rotational data. We report the waveforms and the seismological analysis of some seismic events recorded during our first year of activity inside the LNGS laboratory.

  8. Proteomic identification of biomarkers of vascular injury

    PubMed Central

    Huang, Ngan F; Kurpinski, Kyle; Fang, Qizhi; Lee, Randall J; Li, Song

    2011-01-01

    Predictive biomarkers may be beneficial for detecting, diagnosing, and assessing the risk of restenosis and vascular injury. We utilized proteomic profiling to identify protein markers in the blood following vascular injury, and corroborated the differential protein expression with immunological approaches. Rats underwent carotid artery injury, and plasma was collected after 2 or 5 weeks. Proteomic profiling was carried out by two-dimensional differential in-gel electrophoresis. The differentially expressed plasma proteins were identified by mass spectroscopy and confirmed by immunoblotting. Proteomic profiling by two-dimensional differential in-gel electrophoresis and mass spectroscopy revealed plasma proteins that were differentially expressed at 2 weeks after injury. Among the proteins identified included vitamin D binding protein (VDBP), aldolase A (aldo A), and apolipoproteinE (apoE). Immunoblotting results validated a significant reduction in these proteins in the plasma at 2 or 5 weeks after vascular injury, in comparison to control animals without vascular injury. These findings suggest that VDBP, aldo A, and apoE may be biomarkers for vascular injury, which will have important prognostic and diagnostic implications. PMID:21416056

  9. Metabolism of bupropion by baboon hepatic and placental microsomes

    PubMed Central

    Wang, Xiaoming; Abdelrahman, Doaa R.; Fokina, Valentina M.; Hankins, Gary D.V.; Ahmed, Mahmoud S.; Nanovskaya, Tatiana N.

    2011-01-01

    The aim of this investigation was to determine the biotransformation of bupropion by baboon hepatic and placental microsomes, identify the enzyme(s) catalyzing the reaction(s) and determine its kinetics. Bupropion was metabolized by baboon hepatic and placental microsomes to hydroxybupropion (OH-BUP), threo- (TB) and erythrohydrobupropion (EB). OH-bupropion was the major metabolite formed by hepatic microsomes (Km 36 ± 6 µM, Vmax 258 ± 32 pmol mg protein−1 min−1), however the formation of OH-BUP by placental microsomes was below the limit of quantification. The apparent Km values of bupropion for the formation of TB and EB by hepatic and placental microsomes were similar. The selective inhibitors of CYP2B6 (ticlopidine and phencyclidine) and monoclonal antibodies raised against human CYP2B6 isozyme caused 80% inhibition of OH-BUP formation by baboon hepatic microsomes. The chemical inhibitors of aldo-keto reductases (flufenamic acid), carbonyl reductases (menadione), and 11β-hydroxysteroid dehydrogenases (18β-glycyrrhetinic acid) significantly decreased the formation of TB and EB by hepatic and placental microsomes. Data indicate that CYP2B of baboon hepatic microsomes is responsible for biotransformation of bupropion to OH-BUP, while hepatic and placental short chain dehydrogenases/reductases and to a lesser extent aldo-keto reductases are responsible for the reduction of bupropion to TB and EB. PMID:21570381

  10. Effect of spironolactone on the renin-aldosterone system in rats.

    PubMed

    Jiménez, W; Martínez-Pardo, A; Arroyo, V; Gaya, J; Rivera, F

    1988-09-01

    PRA, PRC and the plasma concentration of aldosterone (Aldo) were measured in rats (Sp-rats) receiving a daily sc injection of Spironolactone, (Sp, 20 mg in olive oil) and in control rats (C-rats) receiving olive oil only. Animals were studied one day after starting treatment, 5 days on treatment or after 5 weeks on the study. PRA, PRC and Aldo were significantly increased in Sp-rats as compared to C-rats throughout all the study. In additional Sp-rats and C-rats, the urine volume, serum Na+ and K+ concentration, Na+ and K+ intake and the urinary excretion of Na+, K+ and aldosterone-18-glucuronide (UAldV) were serially measured during 5 weeks. The total radioactivity plasma clearance after an i.v. bolus injection of 3H-aldosterone was subsequently measured in (5 Sp-rats and 5 C-rats). No significant differences in serum Na+ and K+ concentration and in Na+ and K+ balance were observed between Sp-rats and C-rats. UAldV was significantly higher in Sp-rats than in C-rats during all the study. After 5 weeks on treatment the total radioactivity plasma clearance was significantly higher in Sp-rats than in C-rats. These results indicate that Sp, at high dosage, stimulates renin release and aldosterone secretion by a mechanism unrelated to alterations in Na+ and K+ balance. PMID:3068736

  11. Effects of spironolactone on human blood mononuclear cells: mineralocorticoid receptor independent effects on gene expression and late apoptosis induction

    PubMed Central

    Sønder, Søren Ulrik Salling; Mikkelsen, Marianne; Rieneck, Klaus; Hedegaard, Chris Juul; Bendtzen, Klaus

    2006-01-01

    Spironolactone (SPIR) binds to cytoplasmic mineralocorticoid receptors (MR) and functions as an aldosterone antagonist. Recently, the drug was shown to have an early suppressive effect on several immunoactive and proinflammatory cytokines. To elucidate the mechanism behind this, the four MR-binding steroids SPIR, canrenone, 7α-thiomethyl-spironolactone and aldosterone (ALDO) were investigated for effects on lipopolysaccharide- and phytohemagglutinin-A-activated human blood mononuclear cells. Gene expression was examined after 4 h using microarrays, and SPIR affected 1018 transcripts of the (=) 22,000 probed. In contrast, the SPIR-related steroids affected 17 or fewer transcripts. Combining SPIR and ALDO resulted in 940 affected transcripts, indicating that SPIR has an early gene-regulatory effect independent of MR. The affected genes encode a large number of signalling proteins and receptors, including immunoinflammatory response genes and apoptosis and antiapoptosis genes. Apoptosis was evident in CD3-, CD14- and CD19-positive cells, but only after 18 h of exposure to SPIR. The transcriptional network involving the differentially regulated genes was examined and the results indicate that SPIR affects genes controlled by the transcription factors NF-κB, CEBPβ and MYC. These observations provide new insight into the non-MR-mediated effects of SPIR. PMID:16520746

  12. Idiopathic primary hyperaldosteronism: normalization of plasma aldosterone after one month withdrawal of long-term therapy with aldosterone-receptor antagonist potassium canrenoate.

    PubMed

    Armanini, D; Scaroni, C; Mattarello, M J; Fiore, C; Albiger, N; Sartorato, P

    2005-03-01

    We have re-evaluated 15 patients with idiopathic primary aldosteronism one month after withdrawal of therapy with aldosterone-receptor antagonist potassium canrenoate. Therapy had lasted for 3 to 24 yr. Median blood pressure (BP) in the sitting position at the time of diagnosis was 160/100 (ranges 150-200/95-110 mmHg); while 1 month after withdrawal of therapy median BP was 145/90 (ranges 125-160/80-100 mmHg). One month after withdrawal, the ratio aldosterone (ng/dl)/plasma renin activity (ng/ml/h) in the upright position was increased only in 3 cases (median 18, range 6.1-125). We found a significant inverse correlation between the upright aldosterone/plasma renin activity (aldo/PRA) ratio, 1 month after withdrawal, and the number of years of therapy with potassium canrenoate. We conclude that long-term therapy with the aldosterone-receptor blocker, potassium canrenoate, can normalize the aldo/PRA ratio in many cases of idiopathic primary hyperaldosteronism after one-month withdrawal of the drug. These data are consistent with possible regression of idiopathic primary hyperaldosteronism after long-term therapy with potassium canrenoate, or in alternative to a persistent effect of potassium canrenoate, on aldosterone synthesis. PMID:15952408

  13. The role of atrial natriuretic peptide (ANP) in cold-induced diuresis (CID)

    SciTech Connect

    Agnew, J.W.; Freund, B.J.; DuBose, D.A.; McKay, J.M.; Hashiro, G.M. Tripler Army Medical Center, Honolulu, HI )

    1991-03-11

    The hormonal control of cold-induced diuresis (CID) remains unresolved. This study investigated the role of ANP, plasma vasopressin (AVP), and aldosterone (ALDO) on CID. Four semi-nude men participated in a 210 min exposure to 15C and 29C air, on separate days. These subjects drank 300 mL of water and had an intravenous saline drip throughout both exposures to replace blood and insensible fluid losses. CID was observed in 15C but not in the 29C experiment, as indicated by a greater urine output. In 15C, atrial natriuretic peptide (ANP) increased after 90 min by 41% and remained elevated for 2 h relative to 29C. No differences were observed in AVP between 15C and 29C. In the 15C versus the 29C experiment, ALDO was approximately 37% lower at the pre, 15 and 90 min time periods. Mean arterial blood pressure was generally greater but only significant at 60 min during the 15C versus the 29C experiment. Urinary NA{sup +} excretion was elevated in 15C relative to 29C while no difference in K{sup +} excretion was observed. Although pressure effects may contribute, the observed natriuresis in the absence of a kaliuresis in the cold suggests a physiological role of ANP in CID.

  14. Photoinduced catalytic synthesis of biologically important metabolites from formaldehyde and ammonia under plausible "prebiotic" conditions

    NASA Astrophysics Data System (ADS)

    Delidovich, I. V.; Taran, O. P.; Simonov, A. N.; Matvienko, L. G.; Parmon, V. N.

    2011-08-01

    The article analyzes new and previously reported data on several catalytic and photochemical processes yielding biologically important molecules. UV-irradiation of formaldehyde aqueous solution yields acetaldehyde, glyoxal, glycolaldehyde and glyceraldehyde, which can serve as precursors of more complex biochemically relevant compounds. Photolysis of aqueous solution of acetaldehyde and ammonium nitrate results in formation of alanine and pyruvic acid. Dehydration of glyceraldehyde catalyzed by zeolite HZSM-5-17 yields pyruvaldehyde. Monosaccharides are formed in the course of the phosphate-catalyzed aldol condensation reactions of glycolaldehyde, glyceraldehyde and formaldehyde. The possibility of the direct synthesis of tetroses, keto- and aldo-pentoses from pure formaldehyde due to the combination of the photochemical production of glycolahyde and phosphate-catalyzed carbohydrate chain growth is demonstrated. Erythrulose and 3-pentulose are the main products of such combined synthesis with selectivity up to 10%. Biologically relevant aldotetroses, aldo- and ketopentoses are more resistant to the photochemical destruction owing to the stabilization in hemiacetal cyclic forms. They are formed as products of isomerization of erythrulose and 3-pentulose. The conjugation of the concerned reactions results in a plausible route to the formation of sugars, amino and organic acids from formaldehyde and ammonia under presumed 'prebiotic' conditions.

  15. PREFACE: 11th International Spring Seminar on Nuclear Physics: Shell Model and Nuclear Structure - achievements of the past two decades

    NASA Astrophysics Data System (ADS)

    2015-02-01

    The 11th International Seminar on Nuclear Physics was held in Ischia from May 12 to May 16, 2014. This Seminar was dedicated to Aldo Covello, who has been the promoter of this series of meetings, which started in Sorrento in 1986 and continued with meetings held every two or three years in the Naples area. Aldo's idea was to offer to a group of researchers, actively working in selected fields of Nuclear Physics, the opportunity to confront their points of view in a lively and informal way. The choice for the period of the year, Spring, as well as the sites chosen reflected this intent. The first meeting was of a purely theoretical nature, but it was immediately clear that the scope of these conferences needed to be enlarged calling into play the experimental community. Then, starting from the second meeting, all the following ones have been characterized by fruitful discussion between theoretical and experimental researchers on current achievements and future developments of nuclear structure. This may be read, in fact, as one of the motivating factors for Aldo's election as Fellow of the American Physical Society in 2008 "... for his outstanding contributions to the international nuclear physics community by providing, for over two decades, a venue for theorists and experimentalists to share their latest ideas." The present meeting, organized by Aldo's former students and with the benefit of his suggestions, has maintained this tradition. The title "Shell model and nuclear structure: achievements of the past two decades" recalls that of the 2nd International Spring Seminar "Shell Model and Nuclear Structure: where do we stand?". The main aim of this 11th Seminar was, in fact, to discuss the changes of the past two decades on our view of nuclei in terms of shell structure as well as the perspectives of the shell model, which has been one of the key points in Aldo's research. This point is well accounted by the Opening Speech of Igal Talmi, one of the fathers of the

  16. Combined Use of Etomidate and Dexmedetomidine Produces an Additive Effect in Inhibiting the Secretion of Human Adrenocortical Hormones.

    PubMed

    Gu, Hongbin; Zhang, Mazhong; Cai, Meihua; Liu, Jinfen

    2015-01-01

    BACKGROUND The direct effects of etomidate were investigated on the secretion of cortisol and its precursors by dispersed cells from the adrenal cortex of human of animals. Dexmedetomidine (DEX) is an anesthetic agent that may interfere with cortisol secretion via an unknown mechanism, such as involving inhibition of 11b-hydroxylase and the cholesterol side-chain cleavage enzyme system. The aim of this study was to determine whether dexmedetomidine (DEX) has a similar inhibitory effect on adrenocortical function, and whether combined use of etomidate (ETO) and DEX could produce a synergistic action in inhibiting the secretion of human adrenocortical hormones. MATERIAL AND METHODS Human adrenocortical cells were exposed to different concentrations of ETO and DEX. The dose-effect model between the ETO concentration and the mean secretion of cortisone (CORT) and aldosterone (ALDO) per hour was estimated. RESULTS Hill's equation well-described the dose-effect correlation between the ETO concentration and the amount of ALDO and CORT secretion. When the DEX concentration was introduced into the model by using E0 (basal secretion) as the covariate, the goodness of fit of the ETO-CORT dose-effect model was improved significantly and the objective function value was reduced by 4.55 points (P<0.05). The parameters of the final ETO-ALDO pharmacodynamics model were EC50=9.74, Emax=1.20, E0=1.33, and γ=18.5; the parameters of the final ETO-CORT pharmacodynamics model were EC50=9.49, Emax=8.16, E0=8.57, and γ=37.0. In the presence of DEX, E0 was 8.57-0.0247×(CDEX-4.6), and the other parameters remained unchanged. All parameters but γ were natural logarithm conversion values. CONCLUSIONS Combined use of DEX and ETO reduced ETO's inhibitory E0 (basal secretion) of CORT from human adrenocortical cells in a dose-dependent manner, suggesting that combined use of ETO and DEX produced an additive effect in inhibiting the secretion of human adrenocortical hormones. PMID:26568275

  17. Combined Use of Etomidate and Dexmedetomidine Produces an Additive Effect in Inhibiting the Secretion of Human Adrenocortical Hormones

    PubMed Central

    Gu, Hongbin; Zhang, Mazhong; Cai, Meihua; Liu, Jinfen

    2015-01-01

    Background The direct effects of etomidate were investigated on the secretion of cortisol and its precursors by dispersed cells from the adrenal cortex of human of animals. Dexmedetomidine (DEX) is an anesthetic agent that may interfere with cortisol secretion via an unknown mechanism, such as involving inhibition of 11β-hydroxylase and the cholesterol side-chain cleavage enzyme system. The aim of this study was to determine whether dexmedetomidine (DEX) has a similar inhibitory effect on adrenocortical function, and whether combined use of etomidate (ETO) and DEX could produce a synergistic action in inhibiting the secretion of human adrenocortical hormones. Material/Methods Human adrenocortical cells were exposed to different concentrations of ETO and DEX. The dose-effect model between the ETO concentration and the mean secretion of cortisone (CORT) and aldosterone (ALDO) per hour was estimated. Results Hill’s equation well-described the dose-effect correlation between the ETO concentration and the amount of ALDO and CORT secretion. When the DEX concentration was introduced into the model by using E0 (basal secretion) as the covariate, the goodness of fit of the ETO-CORT dose-effect model was improved significantly and the objective function value was reduced by 4.55 points (P<0.05). The parameters of the final ETO-ALDO pharmacodynamics model were EC50=9.74, Emax=1.20, E0=1.33, and γ=18.5; the parameters of the final ETO-CORT pharmacodynamics model were EC50=9.49, Emax=8.16, E0=8.57, and γ=37.0. In the presence of DEX, E0 was 8.57–0.0247×(CDEX–4.6), and the other parameters remained unchanged. All parameters but γ were natural logarithm conversion values. Conclusions Combined use of DEX and ETO reduced ETO’s inhibitory E0 (basal secretion) of CORT from human adrenocortical cells in a dose-dependent manner, suggesting that combined use of ETO and DEX produced an additive effect in inhibiting the secretion of human adrenocortical hormones. PMID

  18. Abnormal urinary excretion of NKCC2 and AQP2 in response to hypertonic saline in chronic kidney disease: an intervention study in patients with chronic kidney disease and healthy controls

    PubMed Central

    2014-01-01

    Background Renal handling of sodium and water is abnormal in chronic kidney disease (CKD). The aim of this study was to test the hypothesis that abnormal activity of the aquaporin-2 water channels (AQP2), the sodium-potassium-2chloride transporter (NKCC2) and/or the epithelial sodium channels (ENaC) contribute to this phenomenon. Methods 23 patients with CKD and 24 healthy controls at baseline and after 3% saline infusion were compared. The following measurements were performed: urinary concentrations of AQP2 (u-AQP2), NKCC2 (u-NKCC2), ENaC (u-ENaCγ), glomerular filtration rate (GFR) estimated by 51Cr-EDTA clearance, free water clearance (CH2O), urinary output (UO), fractional excretion of sodium (FENa), plasma concentrations of AVP, renin (PRC), Angiotensin II (ANG II), Aldosterone (Aldo) and body fluid volumes. Results At baseline, GFR was 34 ml/min in CKD patients and 89 ml/ml in controls. There were no significant differences in u-AQP2, u-NKCC2 or u-ENaCγ, but FENa, p-Aldo and p-AVP were higher in CKD patients than controls. In response to hypertonic saline, patients with CKD had an attenuated decrease in CH2O and UO. A greater increase in U-AQP2 was observed in CKD patients compared to controls. Furthermore, u-NKCC2 increased in CKD patients, whereas u-NKCC2 decreased in controls. Body fluid volumes did not significantly differ. Conclusions In response to hypertonic saline, u-NKCC2 increased, suggesting an increased sodium reabsorption via NKCC2 in patients with CKD. U-AQP2 increased more in CKD patients, despite an attenuated decrease in CH2O. Thus, though high levels of p-AVP and p-Aldo, the kidneys can only partly compensate and counteract acute volume expansion due to a defective tubular response. Trial registration Clinical trial no: NCT01623661. Date of trial registration: 18.06.2012. PMID:24970686

  19. Testing SLURM open source batch system for a Tierl/Tier2 HEP computing facility

    NASA Astrophysics Data System (ADS)

    Donvito, Giacinto; Salomoni, Davide; Italiano, Alessandro

    2014-06-01

    In this work the testing activities that were carried on to verify if the SLURM batch system could be used as the production batch system of a typical Tier1/Tier2 HEP computing center are shown. SLURM (Simple Linux Utility for Resource Management) is an Open Source batch system developed mainly by the Lawrence Livermore National Laboratory, SchedMD, Linux NetworX, Hewlett-Packard, and Groupe Bull. Testing was focused both on verifying the functionalities of the batch system and the performance that SLURM is able to offer. We first describe our initial set of requirements. Functionally, we started configuring SLURM so that it replicates all the scheduling policies already used in production in the computing centers involved in the test, i.e. INFN-Bari and the INFN-Tier1 at CNAF, Bologna. Currently, the INFN-Tier1 is using IBM LSF (Load Sharing Facility), while INFN-Bari, an LHC Tier2 for both CMS and Alice, is using Torque as resource manager and MAUI as scheduler. We show how we configured SLURM in order to enable several scheduling functionalities such as Hierarchical FairShare, Quality of Service, user-based and group-based priority, limits on the number of jobs per user/group/queue, job age scheduling, job size scheduling, and scheduling of consumable resources. We then show how different job typologies, like serial, MPI, multi-thread, whole-node and interactive jobs can be managed. Tests on the use of ACLs on queues or in general other resources are then described. A peculiar SLURM feature we also verified is triggers on event, useful to configure specific actions on each possible event in the batch system. We also tested highly available configurations for the master node. This feature is of paramount importance since a mandatory requirement in our scenarios is to have a working farm cluster even in case of hardware failure of the server(s) hosting the batch system. Among our requirements there is also the possibility to deal with pre-execution and post

  20. Thermo-optical vacuum testing of Galileo In-Orbit Validation laser retroreflectors

    NASA Astrophysics Data System (ADS)

    Dell'Agnello, S.; Boni, A.; Cantone, C.; Ciocci, E.; Contessa, S.; Delle Monache, G.; Lops, C.; Martini, M.; Patrizi, G.; Porcelli, L.; Salvatori, L.; Tibuzzi, M.; Intaglietta, N.; Tuscano, P.; Mondaini, C.; Maiello, M.; Doyle, D.; García-Prieto, R.; Navarro-Reyes, D.

    2016-06-01

    The Galileo constellation is a space research and development program of the European Union to help navigate users all over the world. The Galileo IOV (In-Orbit Validation) are the first test satellites of the Galileo constellation and carry satellite laser retroreflectors as part of their payload systems for precision orbit determination and performance assessment. INFN-LNF SCF_Lab (Satellite/lunar/GNSS laser ranging/altimetry and Cube/microsat Characterization Facilities Laboratory) has been performing tests on a sample of the laser array segment under the Thermo-optical vacuum testing of Galileo IOV laser retro-reflectors of Galileo IOV LRA project, as defined in ESA-INFN Contract No. 4000108617/13/NL/PA. We will present the results of FFDP (Far Field Diffraction Pattern) and thermal relaxation times measurements in relevant space conditions of Galileo IOV CCRs (Cube Corner Retroreflectors) provided by ESA-ESTEC. A reference for the performance of laser ranging on Galileo satellites is the FFDP of a retroreflector in its design specifications and a Galileo retroreflector, in air and isothermal conditions, should have a minimum return intensity within the range [ 0.55 ×106m2- 2.14 ×106m2 ] (ESA-INFN, 2013). Measurements, performed in SCF_Lab facility, demonstrated that the 7 Galileo IOV laser retroreflectors under test were compliant with design performance expectations (Porcelli et al., 2015). The kind of tests carried out for this activity are the first performed on spare Galileo IOV hardware, made available after the launch of the four Galileo IOV satellites (2011 and 2012), which were the operational core of the constellation. The characterisation of the retroreflectors against their design requirements is important because LRAs (Laser Retroreflector Arrays) will be flown on all Galileo satellites.

  1. Implementation and use of a highly available and innovative IaaS solution: the Cloud Area Padovana

    NASA Astrophysics Data System (ADS)

    Aiftimiei, C.; Andreetto, P.; Bertocco, S.; Biasotto, M.; Dal Pra, S.; Costa, F.; Crescente, A.; Dorigo, A.; Fantinel, S.; Fanzago, F.; Frizziero, E.; Gulmini, M.; Michelotto, M.; Sgaravatto, M.; Traldi, S.; Venaruzzo, M.; Verlato, M.; Zangrando, L.

    2015-12-01

    While in the business world the cloud paradigm is typically implemented purchasing resources and services from third party providers (e.g. Amazon), in the scientific environment there's usually the need of on-premises IaaS infrastructures which allow efficient usage of the hardware distributed among (and owned by) different scientific administrative domains. In addition, the requirement of open source adoption has led to the choice of products like OpenStack by many organizations. We describe a use case of the Italian National Institute for Nuclear Physics (INFN) which resulted in the implementation of a unique cloud service, called ’Cloud Area Padovana’, which encompasses resources spread over two different sites: the INFN Legnaro National Laboratories and the INFN Padova division. We describe how this IaaS has been implemented, which technologies have been adopted and how services have been configured in high-availability (HA) mode. We also discuss how identity and authorization management were implemented, adopting a widely accepted standard architecture based on SAML2 and OpenID: by leveraging the versatility of those standards the integration with authentication federations like IDEM was implemented. We also discuss some other innovative developments, such as a pluggable scheduler, implemented as an extension of the native OpenStack scheduler, which allows the allocation of resources according to a fair-share based model and which provides a persistent queuing mechanism for handling user requests that can not be immediately served. Tools, technologies, procedures used to install, configure, monitor, operate this cloud service are also discussed. Finally we present some examples that show how this IaaS infrastructure is being used.

  2. PREFACE: International Seminar on Strong and Electromagnetic Interactions in High Energy Collisions 2012

    NASA Astrophysics Data System (ADS)

    Giardina, Giorgio; Sandorfi, Andrew; Pedroni, Paolo

    2013-03-01

    The International Seminar 'Strong and Electromagnetic Interaction in High Energy Collisions' was held in the Conference Hall 'Ettore Majorana' of the Department of Physics in Messina, Italy on October 12, 2012. The Seminar was organized by the University of Messina and 'Fondazione Bonino-Pulejo', with the aim of presenting and discussing the results of the current experiments and also new plans involving research at INFN-LNF (Italy), JLAB (USA), LHC-CERN, ELSA (Bonn), MAMI (Mainz). The main purpose of this Seminar was to deal with aspects of electromagnetic and strong forces by meson photoproduction and the electron-positron collider, and to search for dark energy. The recent results on hadron contributions to the muon anomalous magnetic moment and kaon interferometry at the DAFNE facility were also discussed. Editors: Giorgio Giardina (University of Messina), Andrew M Sandorfi (Thomas Jefferson National Accelerator Facility, Newport News, USA), Paolo Pedroni (INFN 'Sezione di Pavia') Organizing Committee: Chairman: G Giardina (Messina - Italy) Co-Chairman: A M Sandorfi (Newport News, USA) Co-Chairman: P Pedroni (Pavia - Italy) Scientific Secretary: G Mandaglio (University of Messina - Italy) Organizing Institutions: University of Messina Fondazione Bonino-Pulejo (Messina) Topics: Meson photoproduction and baryon resonances Muon anomaly (g-2) Recent results in experiments at the Large Hadron Collider Kaon interferometry Local Organizing Committee: F Curciarello, V De Leo, G Fazio, G Giardina, G Mandaglio, M Romaniuk Sponsored by: University of Messina, Fondazione Bonino-Pulejo (Messina), INFN Sezione di Catania Web-Site: http://newcleo.unime.it/IntSem2012

  3. The FLUKA Code: an Overview

    SciTech Connect

    Ballarini, F.; Battistoni, G.; Campanella, M.; Carboni, M.; Cerutti, F.; Empl, A.; Fasso, A.; Ferrari, A.; Gadioli, E.; Garzelli, M.V.; Lantz, M.; Liotta, M.; Mairani, A.; Mostacci, A.; Muraro, S.; Ottolenghi, A.; Pelliccioni, M.; Pinsky, L.; Ranft, J.; Roesler, S.; Sala, P.R.; /Milan U. /INFN, Milan /Pavia U. /INFN, Pavia /CERN /Siegen U. /Houston U. /SLAC /Frascati /NASA, Houston /ENEA, Frascati

    2005-11-09

    FLUKA is a multipurpose Monte Carlo code which can transport a variety of particles over a wide energy range in complex geometries. The code is a joint project of INFN and CERN: part of its development is also supported by the University of Houston and NASA. FLUKA is successfully applied in several fields, including but not only, particle physics, cosmic ray physics, dosimetry, radioprotection, hadron therapy, space radiation, accelerator design and neutronics. The code is the standard tool used at CERN for dosimetry, radioprotection and beam-machine interaction studies. Here we give a glimpse into the code physics models with a particular emphasis to the hadronic and nuclear sector.

  4. Monolithic pixel detectors in silicon on insulator technology

    NASA Astrophysics Data System (ADS)

    Bisello, Dario

    2013-05-01

    Silicon On Insulator (SOI) is becoming an attractive technology to fabricate monolithic pixel detectors. The possibility of using the depleted resistive substrate as a drift collection volume and to connect it by means of vias through the buried oxide to the pixel electronic makes this kind of approach interesting both for particle and photon detection. In this paper I report the results obtained in the development of monolithic pixel detectors in an SOI technology by a collaboration between groups from the University and INFN of Padova (Italy) and the LBNL and the SCIPP at UCSC (USA).

  5. The new hybrid thermal neutron facility at TAPIRO reactor for BNCT radiobiological experiments.

    PubMed

    Esposito, J; Rosi, G; Agosteo, S

    2007-01-01

    A new thermal neutron irradiation facility, devoted to carry out both dosimetric and radiobiological studies on boron carriers, which are being developed in the framework of INFN BNCT project, has been installed at the ENEA Casaccia TAPIRO research fast reactor. The thermal column, based on an original, hybrid, neutron spectrum shifter configuration, has been recently become operative. In spite of its low power (5 kW), the new facility is able to provide a high thermal neutron flux level, uniformly distributed inside the irradiation cavity, with a quite low gamma background. The main features and preliminary benchmark measurements of the Beam-shaping assembly are here presented and discussed. PMID:17504745

  6. Beam test results for a tungsten-cerium fluoride sampling calorimeter with wavelength-shifting fiber readout

    NASA Astrophysics Data System (ADS)

    Becker, R.; Candelise, V.; Cavallari, F.; Dafinei, I.; Della Ricca, G.; Diemoz, M.; del Re, D.; D'Imperio, G.; Dissertori, G.; Donegà, M.; Dröge, M.; Gelli, S.; Haller, C.; Jorda Lope, C.; Lustermann, W.; Martelli, A.; Meridiani, P.; Micheli, F.; Nessi-Tedaldi, F.; Nuccetelli, M.; Organtini, G.; Quittnat, M.; Pandolfi, F.; Paramatti, R.; Pastrone, N.; Pellegrino, F.; Peruzzi, M.; Rahatlou, S.; Rovelli, C.; Santanastasio, F.; Schönenberger, M.; Soffi, L.; Tabarelli de Fatis, T.; Vazzoler, F.

    2015-07-01

    A sampling calorimeter using cerium fluoride scintillating crystals as active material, interleaved with heavy absorber plates, and read out by wavelength-shifting (WLS) fibers is being studied as a calorimeter option for detectors at the upgraded High-Luminosity LHC (HL-LHC) collider at CERN. A prototype has been exposed to electron beams of different energies at the INFN Frascati (Italy) Beam Test Facility. This paper presents results from the studies performed on the prototype, such as signal amplitudes, light yield and energy resolution.

  7. Uranium Groundwater Monitoring and Seismic Analysis: A Case Study of the Gran Sasso Hydrogeological Basin, Italy

    NASA Astrophysics Data System (ADS)

    Ciarletti, Marta; Plastino, Wolfango; Peresan, Antonella; Nisi, Stefano; Copia, Lorenzo; Panza, Giuliano F.; Povinec, Pavel P.

    2016-04-01

    Uranium groundwater anomalies, observed before the L'Aquila earthquake (April 6th, 2009) and before the seismic swarm, which occurred in the second half of 2010, represent a key geochemical signal of a progressive increase of deep fluids fluxes at middle-lower crustal levels associated with the geodynamics of the earthquake. In this paper, temporal variations of uranium groundwater are studied in association with the seismic pattern around Gran Sasso National Laboratory (LNGS-INFN). The normalized seismic energy release and the number of earthquakes are analyzed in detail by means of monthly sliding time windows. They are compared with uranium anomalies to highlight any possible correlation.

  8. Status report of the GERDA experiment phase I

    NASA Astrophysics Data System (ADS)

    Riboldi, Stefano; Gerda Collaboration

    2013-08-01

    Phase I of GERDA, aimed at investigating neutrino-less double beta decay of 76Ge is in the active phase since November 2011 at the Gran Sasso National Laboratory of INFN-Italy. GERDA Ge detectors are non-encapsulated and operate immersed in liquid argon, equipped with a front-end readout electronics consisting of cryogenic charge sensitive preamplifiers designed and manufactured to cope with the characteristics of the GERDA experiment (radio-purity, long and resistive cables, etc.). The presentation will report on the current status of the GERDA experiment phase I, focusing on Ge detectors performance in terms of energy resolution, stability over time, counting rate and related issues.

  9. GERDA - Status and Perspectives

    NASA Astrophysics Data System (ADS)

    Knöpfle, K. T.

    2013-04-01

    The GERmanium Detector Array (GERDA) experiment has been built at the INFN National Gran Sasso Laboratory (LNGS) for the search of neutrinoless double beta (0νββ) decay in 76Ge. It is implementing a new shielding concept by operating bare Ge diodes, enriched in 76Ge, in high purity liquid argon supplemented by a water shield. Phase I data taking of GERDA has started in November 2011. This paper presents preliminary data showing the performance achieved so far, and outlines the preparations for Phase II that aims at reducing backgrounds by another factor of ten.

  10. Results on decay with emission of two neutrinos or Majorons in Ge from GERDA Phase I

    NASA Astrophysics Data System (ADS)

    Agostini, M.; Allardt, M.; Bakalyarov, A. M.; Balata, M.; Barabanov, I.; Barros, N.; Baudis, L.; Bauer, C.; Becerici-Schmidt, N.; Bellotti, E.; Belogurov, S.; Belyaev, S. T.; Benato, G.; Bettini, A.; Bezrukov, L.; Bode, T.; Borowicz, D.; Brudanin, V.; Brugnera, R.; Budjáš, D.; Caldwell, A.; Cattadori, C.; Chernogorov, A.; D'Andrea, V.; Demidova, E. V.; di Vacri, A.; Domula, A.; Doroshkevich, E.; Egorov, V.; Falkenstein, R.; Fedorova, O.; Freund, K.; Frodyma, N.; Gangapshev, A.; Garfagnini, A.; Grabmayr, P.; Gurentsov, V.; Gusev, K.; Hegai, A.; Heisel, M.; Hemmer, S.; Heusser, G.; Hofmann, W.; Hult, M.; Inzhechik, L. V.; Csáthy, J. Janicskó; Jochum, J.; Junker, M.; Kazalov, V.; Kihm, T.; Kirpichnikov, I. V.; Kirsch, A.; Klimenko, A.; Knöpfle, K. T.; Kochetov, O.; Kornoukhov, V. N.; Kuzminov, V. V.; Laubenstein, M.; Lazzaro, A.; Lebedev, V. I.; Lehnert, B.; Liao, H. Y.; Lindner, M.; Lippi, I.; Lubashevskiy, A.; Lubsandorzhiev, B.; Lutter, G.; Macolino, C.; Majorovits, B.; Maneschg, W.; Medinaceli, E.; Misiaszek, M.; Moseev, P.; Nemchenok, I.; Palioselitis, D.; Panas, K.; Pandola, L.; Pelczar, K.; Pullia, A.; Riboldi, S.; Rumyantseva, N.; Sada, C.; Salathe, M.; Schmitt, C.; Schneider, B.; Schönert, S.; Schreiner, J.; Schütz, A.-K.; Schulz, O.; Schwingenheuer, B.; Selivanenko, O.; Shirchenko, M.; Simgen, H.; Smolnikov, A.; Stanco, L.; Stepaniuk, M.; Ur, C. A.; Vanhoefer, L.; Vasenko, A. A.; Veresnikova, A.; von Sturm, K.; Wagner, V.; Walter, M.; Wegmann, A.; Wester, T.; Wilsenach, H.; Wojcik, M.; Yanovich, E.; Zavarise, P.; Zhitnikov, I.; Zhukov, S. V.; Zinatulina, D.; Zuber, K.; Zuzel, G.

    2015-09-01

    A search for neutrinoless decay processes accompanied with Majoron emission has been performed using data collected during Phase I of the GERmanium Detector Array (GERDA) experiment at the Laboratori Nazionali del Gran Sasso of INFN (Italy). Processes with spectral indices were searched for. No signals were found and lower limits of the order of 10 yr on their half-lives were derived, yielding substantially improved results compared to previous experiments with Ge. A new result for the half-life of the neutrino-accompanied decay of Ge with significantly reduced uncertainties is also given, resulting in yr.

  11. RIB in-flight production and the facility EXOTIC at LNL

    SciTech Connect

    Mazzocco, Marco

    2014-05-09

    The production of Radioactive Ion Beams (RIBs) via the In-Flight technique is reviewed. This separation method typically employs four main production mechanisms: (i) Projectile Fragmentation; (ii) Projectile Fission; (iii) Nuclear Fusion and (iv) Direct Processes in Inverse Kinematics. We will concentrate particularly on the last mechanism, the one used by the facility EXOTIC at the INFN-Laboratori Nazionali di Legnaro (LNL) for the production of light RIBs. An extensive description of the facility and a brief overview of the most recent scientific achievements with {sup 7}Be and {sup 17}F are given.

  12. NUMEN Project @ LNS : Heavy ions double charge exchange reactions towards the 0νββ nuclear matrix element determination

    SciTech Connect

    Agodi, C. Calabretta, L.; Calanna, A.; Carbone, D.; Cavallaro, M.; Colonna, M.; Cuttone, G.; Finocchiaro, P.; Pandola, L.; Rifuggiato, D.; Tudisco, S.; Cappuzzello, F.; Greco, V.; Bonanno, D. L.; Bongiovanni, D. G.; Longhitano, F.; Branchina, V.; Foti, A.; Lo Presti, D.; Lanzalone, G.; and others

    2015-10-28

    In the NUMEN Project it is proposed an innovative technique to access the nuclear matrix elements entering in the expression of the life-time of the neutrinoless double beta decay, using relevant cross sections of double charge exchange reactions. A key aspect is the use of MAGNEX large acceptance magnetic spectrometer, for the detection of the ejectiles, and of the INFN Laboratori Nazionali del Sud (LNS) K800 Superconducting Cyclotron (CS), for the acceleration of the required high resolution and low emittance heavy-ion beams.

  13. Ion acceleration with a narrow energy spectrum by nanosecond laser-irradiation of solid target

    NASA Astrophysics Data System (ADS)

    Altana, C.; Lanzalone, G.; Mascali, D.; Muoio, A.; Cirrone, G. A. P.; Schillaci, F.; Tudisco, S.

    2016-02-01

    In laser-driven plasma, ion acceleration of aluminum with the production of a quasi-monoenergetic beam has occurred. A useful device to analyze the ions is the Thomson parabolas spectrometer, a well-known diagnostic that is able to obtain information on charge-to-mass ratio and energy distribution of the charged particles. At the LENS (Laser Energy for Nuclear Science) laboratory of INFN-LNS in Catania, experimental measures were carried out; the features of LENS are: Q-switched Nd:YAG laser with 2 J laser energy, 1064 nm fundamental wavelengths, and 6 ns pulse duration.

  14. The design of the optical modules of the KM3NeT-Italia project towers

    NASA Astrophysics Data System (ADS)

    Leonora, Emanuele; Aiello, Sebastiano; Giordano, Valentina

    2016-07-01

    The KM3NeT-Italia project aims to construct a large volume underwater neutrino telescope, to be installed in the depths of the Mediterranean Sea. The R&D and mass production phases of the detection elements of the telescope, the optical modules, were entirely performed in the INFN-LNS site in the harbour of Catania. In November 2014 a first tower of 14 storeys equipped with 84 optical modules was successfully deployed in the Mediterranean Sea site. The design of the optical modules and their main components are described in this paper.

  15. Injection of auxiliary electrons for increasing the plasma density in highly charged and high intensity ion sources.

    PubMed

    Odorici, F; Malferrari, L; Montanari, A; Rizzoli, R; Mascali, D; Castro, G; Celona, L; Gammino, S; Neri, L

    2016-02-01

    Different electron guns based on cold- or hot-cathode technologies have been developed since 2009 at INFN for operating within ECR plasma chambers as sources of auxiliary electrons, with the aim of boosting the source performances by means of a higher plasma lifetime and density. Their application to microwave discharge ion sources, where plasma is not confined, has required an improvement of the gun design, in order to "screen" the cathode from the plasma particles. Experimental tests carried out on a plasma reactor show a boost of the plasma density, ranging from 10% to 90% when the electron guns are used, as explained by plasma diffusion models. PMID:26931958

  16. Injection of auxiliary electrons for increasing the plasma density in highly charged and high intensity ion sources

    NASA Astrophysics Data System (ADS)

    Odorici, F.; Malferrari, L.; Montanari, A.; Rizzoli, R.; Mascali, D.; Castro, G.; Celona, L.; Gammino, S.; Neri, L.

    2016-02-01

    Different electron guns based on cold- or hot-cathode technologies have been developed since 2009 at INFN for operating within ECR plasma chambers as sources of auxiliary electrons, with the aim of boosting the source performances by means of a higher plasma lifetime and density. Their application to microwave discharge ion sources, where plasma is not confined, has required an improvement of the gun design, in order to "screen" the cathode from the plasma particles. Experimental tests carried out on a plasma reactor show a boost of the plasma density, ranging from 10% to 90% when the electron guns are used, as explained by plasma diffusion models.

  17. Isospin Effects in Heavy-Ion Collisions: Some Results From CHIMERA Experiments At LNS And Perspectives With Radioactive Beams

    SciTech Connect

    Cardella, G.; De Filippo, E.; Pagano, A.; Papa, M.; Pirrone, S.; Verde, G.; Amorini, F.; Cavallaro, S.; Lombardo, I.; Porto, F.; Rizzo, F.; Russotto, P.; Anzalone, A.; Maiolino, C.; Arena, N.; Geraci, E.; Grassi, L.; Lo Nigro, S.; Politi, G.; Auditore, L.

    2009-05-04

    CHIMERA is a 4{pi} multidetector for charged particles available at Laboratori Nazionali del Sud (INFN-LNS). A new method to measure the time scale of the emission of nuclear fragments is described, together with some applications in the field of the isospin dynamics of heavy-ion collisions. Competition between fusion-like and binary reactions near the energy threshold for nuclear multifragmentation is discussed. Opportunities are pointed out to use the detector at low and intermediate energies using the kinematical-coincidence method.

  18. Campaign of measurements to probe the good performance of the new array FARCOS for spectroscopy and correlations.

    NASA Astrophysics Data System (ADS)

    Acosta, L.; Andolina, R.; Auditore, L.; Boiano, C.; Cardella, G.; Castoldi, A.; D'Andrea, M.; De Filippo, E.; De Luca, S.; Dell'Aquila, D.; Francalanza, L.; Gnoffo, B.; Guazzoni, C.; Lanzalone, G.; Lombardo, I.; Martorana, N.; Minniti, T.; Norella, S.; Pagano, A.; Pagano, E. V.; Papa, M.; Parsani, T.; Pirrone, S.; Politi, G.; Porto, F.; Quattrocchi, L.; Rizzo, F.; Russotto, P.; Saccá, G.; Trifirò, A.; Trimarchi, M.; Verde, G.; Vigilante, M.; Zambon, P.

    2016-07-01

    During the last four years, several measurements have been carried out where the capabilities of FARCOS array were tested. In some of this occasions, FARCOS was coupled to the 4π array CHIMERA, permanently placed at INFN-Laboratori Nazionali del Sud, Catania in order to be tested in real experimental measurements. At the present situation, the FARCOS demonstrator is formed by 4 telescopes out of the originally 20 that will constitute the final array. Here are presented some preliminary results obtained with the new array, probing its qualities and showing the effectiveness of FARCOS telescopes. The initial encouraging results support the construction of the complete array.

  19. A multigap resistive plate chamber array for the Extreme Energy Events project

    NASA Astrophysics Data System (ADS)

    De Gruttola, D.; Abbrescia, M.; Agocs, A.; Aiola, S.; Antolini, R.; Avanzini, C.; Baldini Ferroli, R.; Bencivenni, G.; Bossini, E.; Bressan, E.; Chiavassa, A.; Cicalò, C.; Cifarelli, L.; Coccia, E.; De Pasquale, S.; Di Giovanni, A.; D'Incecco, M.; Dreucci, M.; Fabbri, F. L.; Frolov, V.; Garbini, M.; Gemme, G.; Gnesi, I.; Gustavino, C.; Hatzifotiadou, D.; La Rocca, P.; Li, S.; Librizzi, F.; Maggiora, A.; Massai, M.; Miozzi, S.; Panareo, M.; Paoletti, R.; Perasso, L.; Pilo, F.; Piragino, G.; Regano, A.; Riggi, F.; Righini, G. C.; Sartorelli, G.; Scapparone, E.; Scribano, A.; Selvi, M.; Serci, S.; Siddi, E.; Spandre, G.; Squarcia, S.; Taiuti, M.; Tosello, F.; Votano, L.; Williams, M. C. S.; Yanez, G.; Zichichi, A.; Zouyevski, R.

    2014-10-01

    The Extreme Energy Events (EEE) Project is a Centro Fermi - CERN - INFN - MIUR Collaboration Project for the study of extremely high energy cosmic rays, which exploits the Multigap Resistive Plate Chamber (MRPC) technology. The excellent time resolution and good tracking capability of this kind of detector allows us to study Extensive Air Showers (EAS) with an array of MRPC telescopes distributed across the Italian territory. Each telescope is installed in a high school, with the further goal to introduce students to particle and astroparticle Physics. The status of the experiment and the results obtained are reported.

  20. ({sup 18}O,{sup 18}Ne) double charge-exchange with MAGNEX

    SciTech Connect

    Bondí, M.; Cappuzzello, F.; Nicolosi, D.; Tropea, S.; Agodi, C.; Carbone, D.; Cavallaro, M.; Cunsolo, A.; De Napoli, M.; Foti, A.

    2014-05-09

    An experimental study concerning Double Gamow-Teller (DGT) modes in ({sup 18}O,{sup 18}Ne) Double Charge-Exchange reactions has been very recently performed at INFN-LNS laboratory in Catania. The experiment was performed using a {sup 40}Ca solid target and a {sup 18}O Cyclotron beam at 270 MeV incident energy. Charged ejectiles produced in the reaction were momentum analyzed and identified by MAGNEX spectrometer at very forward angles. Preliminary results are presented in the present paper.

  1. Study of the hadronic interactions of kaons in light nuclei at DA{Phi}ne

    SciTech Connect

    Vazquez Doce, O.

    2010-08-05

    The AMADEUS experiment at the Da{Phi}ne accelerator of the Frascati National Laboratories (Italy) of INFN, will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, with a complete experimental program for the case of the kaonic clusters. Studying the absorption of antikaon by the nucleus will provide information concerning the K-barN interaction and the modification of the kaon mass in the nuclear medium.A preliminar study of these kind of hadronic interactions is being done by the AMADEUS collaboration by analyzing the existent KLOE data.

  2. A 16 channel frequency-domain-modulation readout system with custom superconducting LC filters for the SWIPE instrument of the balloon-borne LSPE experiment

    NASA Astrophysics Data System (ADS)

    Signorelli, G.; Baldini, A. M.; Bemporad, C.; Biasotti, M.; Cei, F.; Ceriale, V.; Corsini, D.; Fontanelli, F.; Galli, L.; Gallucci, G.; Gatti, F.; Incagli, M.; Grassi, M.; Nicolò, D.; Spinella, F.; Vaccaro, D.; Venturini, M.

    2016-07-01

    We present the design, implementation and first tests of the superconducting LC filters for the frequency domain readout of spiderweb TES bolometers of the SWIPE experiment on the balloon-borne LSPE mission which aims at measuring the linear polarization of the Cosmic Microwave Background at large angular scales to find the imprint of inflation on the B-mode CMB polarization. LC filters are designed, produced and tested at the INFN sections of Pisa and Genoa where thin film deposition and cryogenic test facilities are present, and where also the TES spiderweb bolometers are being produced.

  3. Mono-energetic ions emission by nanosecond laser solid target irradiation

    NASA Astrophysics Data System (ADS)

    Muoio, A.; Tudisco, S.; Altana, C.; Lanzalone, G.; Mascali, D.; Cirrone, G. A. P.; Schillaci, F.; Trifirò, A.

    2016-09-01

    An experimental campaign aiming to investigate the acceleration mechanisms through laser-matter interaction in nanosecond domain has been carried out at the LENS (Laser Energy for Nuclear Science) laboratory of INFN-LNS, Catania. Pure Al targets were irradiated by 6 ns laser pulses at different pumping energies, up to 2 J. Advanced diagnostics tools were used to characterize the plasma plume and ion production. We show the preliminary results of this experimental campaign, and especially the ones showing the production of multicharged ions having very narrow energy spreads.

  4. PREFACE: Nanoscale science and technology

    NASA Astrophysics Data System (ADS)

    Bellucci, Stefano

    2008-11-01

    Over the last decade, rapid progress in the field of nanoscience has been increasingly driving the attention of the scientific community as well as society at large on the corresponding technological applications, which are the object of so-called nanotechnology. A strong interest in assessing the current state of the art of this fast growing field, as well as stimulating research networking, prompted the organization of the International School and Workshop 'Nanoscience & Nanotechnology (n&n2007)', under the patronage of the Italian Institute for Nuclear Physics (INFN), the University of Rome Tor Vergata, the Tor Vergata Polyclinic, and the Catholic University of Rome, with generous sponsorship from 3M, 2M Strumenti, MTS, Ape Research, Crisel Instruments, Veeco and Amira. The aims of this event were as follows: to foster the concrete planning of future devices based on innovative (nano)materials, involving both industrial entities and public research institutes; to allow sponsoring firms to present their instrumentation and success stories, based on current use by significant customers; to lend an opportunity for preparing and presenting joint projects, involving both industry and public research, see e.g. the EU 7th Framework Programs; to explore the possibility of integrating nanodevices from their concept into system projects. The conference http://www.lnf.infn.it/conference/nn2007/ gathered at Villa Mondragone in Monte Porzio Catone, Italy, with leading experts in research and innovative technologies in biology, medicine, aerospace, optoelectronics, materials and instrumentation, coming both from academic research and industrial areas, as well as national security and military defense experts in attendence. Several successful meetings in this field have taken place in the past, such as the Nanotubes & Nanostructures (N&N) School and Workshop Series [1, 2, 3, 4] including: N&N2000, S Margherita di Pula (Cagliari), Italy, 24 September-4 October 2000, http://www.lnf.infn

  5. The Archimedes experiment

    NASA Astrophysics Data System (ADS)

    Calloni, E.; Caprara, S.; Laurentis, M. De; Esposito, G.; Grilli, M.; Majorana, E.; Pepe, G. P.; Petrarca, S.; Puppo, P.; Rapagnani, P.; Ricci, F.; Rosa, L.; Rovelli, C.; Ruggi, P.; Saini, N. L.; Stornaiolo, C.; Tafuri, F.

    2016-07-01

    Archimedes is an INFN-funded pathfinder experiment aimed at verifying the feasibility of measuring the interaction of vacuum fluctuations with gravity. The final experiment will measure the force exerted by the gravitational field on a Casimir cavity whose vacuum energy is modulated with a superconductive transition, by using a balance as a small force detector. Archimedes is two-year project devoted to test the most critical experimental aspects, in particular the balance resonance frequency and quality factor, the thermal modulation efficiency and the superconductive sample realization.

  6. Laser-capillary interaction for the EXIN project

    NASA Astrophysics Data System (ADS)

    Bisesto, F. G.; Anania, M. P.; Bacci, A. L.; Bellaveglia, M.; Chiadroni, E.; Cianchi, A.; Curcio, A.; Di Giovenale, D.; Di Pirro, G.; Ferrario, M.; Galletti, M.; Gallo, A.; Ghigo, A.; Marocchino, A.; Mostacci, A.; Petrarca, M.; Pompili, R.; Rossi, A. R.; Serafini, L.; Vaccarezza, C.

    2016-09-01

    The EXIN project is under development within the SPARC_LAB facility of the National Laboratory of Frascati (LNF-INFN). This project aims to accelerate pre-existing electron bunches with high brightness by exploiting the wakefield plasma acceleration technique, while preserving the initial brightness. The wakefield is excited inside a dielectric capillary by high intensity laser pulses produced by the FLAME laser interacting with a gas. In this work, we present numerical simulations in order to optimize energy coupling between our laser with super-Gaussian transverse profile and a dielectric capillary. Moreover, an overview of the experimental layout will be given.

  7. Implication for the Core-collapse Supernova Rate from 21 Years of Data of the Large Volume Detector

    NASA Astrophysics Data System (ADS)

    Agafonova, N. Y.; Aglietta, M.; Antonioli, P.; Ashikhmin, V. V.; Badino, G.; Bari, G.; Bertoni, R.; Bressan, E.; Bruno, G.; Dadykin, V. L.; Dobrynina, E. A.; Enikeev, R. I.; Fulgione, W.; Galeotti, P.; Garbini, M.; Ghia, P. L.; Giusti, P.; Gomez, F.; Kemp, E.; Malgin, A. S.; Molinario, A.; Persiani, R.; Pless, I. A.; Porta, A.; Ryasny, V. G.; Ryazhskaya, O. G.; Saavedra, O.; Sartorelli, G.; Shakiryanova, I. R.; Selvi, M.; Trinchero, G. C.; Vigorito, C.; Yakushev, V. F.; Zichichi, A.; LVD Collaboration

    2015-03-01

    The Large Volume Detector (LVD) has been continuously taking data since 1992 at the INFN Gran Sasso National Laboratory. The LVD is sensitive to neutrino bursts from gravitational stellar collapses with full detection probability over the Galaxy. We have searched for neutrino bursts in LVD data taken over 7,335 days of operation. No evidence of neutrino signals has been found between 1992 June and 2013 December. The 90% C.L. upper limit on the rate of core collapse and failed supernova explosions out to distances of 25 kpc is found to be 0.114 yr-1.

  8. The FLUKA Code: An Overview

    NASA Technical Reports Server (NTRS)

    Ballarini, F.; Battistoni, G.; Campanella, M.; Carboni, M.; Cerutti, F.; Empl, A.; Fasso, A.; Ferrari, A.; Gadioli, E.; Garzelli, M. V.; Lantz, M.; Liotta, M.; Mairani, A.; Mostacci, A.; Muraro, S.; Ottolenghi, A.; Pelliccioni, M.; Pinsky, L.; Ranft, J.; Roesler, S.; Sala, P. R.; Scannicchio, D.; Trovati, S.; Villari, R.; Wilson, T.

    2006-01-01

    FLUKA is a multipurpose Monte Carlo code which can transport a variety of particles over a wide energy range in complex geometries. The code is a joint project of INFN and CERN: part of its development is also supported by the University of Houston and NASA. FLUKA is successfully applied in several fields, including but not only, particle physics, cosmic ray physics, dosimetry, radioprotection, hadron therapy, space radiation, accelerator design and neutronics. The code is the standard tool used at CERN for dosimetry, radioprotection and beam-machine interaction studies. Here we give a glimpse into the code physics models with a particular emphasis to the hadronic and nuclear sector.

  9. An intrinsically safe facility for forefront research and training on nuclear technologies —Neutron yield from Be

    NASA Astrophysics Data System (ADS)

    Osipenko, M.; Ripani, M.; Ricco, G.; Celentano, A.; Viberti, C. M.; Alba, R.; Schillaci, M.; Cosentino, G.; Del Zoppo, A.; Di Pietro, A.; Figuera, P.; Finocchiaro, P.; Maiolino, C.; Santonocito, D.; Barbagallo, M.; Colonna, N.; Boccaccio, P.; Esposito, J.; Kostyukov, A.

    2014-04-01

    We describe a dedicated experiment to measure the neutron yield produced by a 62MeV proton beam impinging on a beryllium thick target. The energy was chosen as close as possible to the 70MeV considered for the ADS layout described in this Focus Point. The neutron yield and energy spectra were measured at several angles with respect to the beam direction. The experiment was performed at the INFN Laboratori Nazionali del Sud in Catania, Italy, using the proton beam delivered by the Superconducting Cyclotron (CS).

  10. The LILIA experiment: Energy selection and post-acceleration of laser generated protons

    NASA Astrophysics Data System (ADS)

    Turchetti, Giorgio; Sinigardi, Stefano; Londrillo, Pasquale; Rossi, Francesco; Sumini, Marco; Giove, Dario; De Martinis, Carlo

    2012-12-01

    The LILIA experiment is planned at the SPARCLAB facility of the Frascati INFN laboratories. We have simulated the laser acceleration of protons, the transport and energy selection with collimators and a pulsed solenoid and the post-acceleration with a compact high field linac. For the highest achievable intensity corresponding to a = 30 over 108 protons at 30 MeV with a 3% spread are selected, and at least107 protons are post-accelerated up to 60 MeV. If a 10 Hz repetition rated can be achieved the delivered dose would be suitable for the treatment of small superficial tumors.

  11. The LILIA experiment: Energy selection and post-acceleration of laser generated protons

    SciTech Connect

    Turchetti, Giorgio; Sinigardi, Stefano; Londrillo, Pasquale; Rossi, Francesco; Sumini, Marco; Giove, Dario; De Martinis, Carlo

    2012-12-21

    The LILIA experiment is planned at the SPARCLAB facility of the Frascati INFN laboratories. We have simulated the laser acceleration of protons, the transport and energy selection with collimators and a pulsed solenoid and the post-acceleration with a compact high field linac. For the highest achievable intensity corresponding to a= 30 over 10{sup 8} protons at 30 MeV with a 3% spread are selected, and at least10{sup 7} protons are post-accelerated up to 60 MeV. If a 10 Hz repetition rated can be achieved the delivered dose would be suitable for the treatment of small superficial tumors.

  12. Performances of a method for reconstructing the energy of neutrons detected by a double scattering spectrometer

    SciTech Connect

    Agnello, M.; Botta, E.; Bressani, T.; Calvo, D.; Gianotti, P.; Iazzi, F.; Lamberti, C.; Minetti, B. ); Balocco, E. )

    1992-10-01

    This paper reports on a neutron spectrometer based on the double scattering technique which has been designed and built at the Laboratorio Tecnologico of INFN - Turin (Italy) for Cold Fusion experiments. The operating principle for the reconstruction of the energy can be applied to various fields (neutron emission from sources, fission and fusion) and is described together with the performed tests: a resolution of less than 560 KeV FWHM has been obtained for neutrons of 2.45 MeV, in a typical running configuration.

  13. Test beam results with a sampling calorimeter of cerium fluoride scintillating crystals and tungsten absorber plates for calorimetry at the HL-LHC

    NASA Astrophysics Data System (ADS)

    Becker, R.; Dissertori, G.; Djambazov, L.; Donegà, M.; Dröge, M.; Haller, C.; Horisberger, U.; Lustermann, W.; Nessi-Tedaldi, F.; Quittnat, M.; Pandolfi, F.; Peruzzi, M.; Schönenberger, M.; Cavallari, F.; Dafinei, I.; Diemoz, M.; D`Imperio, G.; del Re, D.; Gelli, S.; Jorda Lope, C.; Meridiani, P.; Micheli, F.; Nuccetelli, M.; Organtini, G.; Paramatti, R.; Pellegrino, F.; Rahatlou, S.; Rovelli, C.; Santanastasio, F.; Soffi, L.; Tabarelli de Fatis, T.; Martelli, A.; Monti, V.; Pastrone, N.; Trapani, P. P.; Candelise, V.; Della Ricca, G.

    2016-07-01

    A sampling calorimeter using cerium fluoride scintillating crystals as active material, interleaved with absorber plates made of tungsten, and read out by wavelength-shifting fibres has been tested with high-energy electron beams at the CERN SPS H4 beam line, as well as with lower-energy beams at the INFN Frascati Beam Test Facility in Italy. Energy resolution studies revealed a low stochastic term (< 10 % /√{ E }). This result, combined with high radiation hardness of the material used, marks this sampling calorimeter as a good candidate for the detectors' forward regions during the high luminosity phase of LHC.

  14. RF and Magnetic Measurements on the SPARC Photoinjector and Solenoid at UCLA

    SciTech Connect

    Rosenzweig, J.B.; Cook, A.M.; Dunning, M.P.; Frigola, P.; Travish, G.; Sanelli, C.; Tazzioli, F.; Palmer, D.T.; /SLAC

    2006-01-30

    The rf photocathode gun and the solenoid for the SPARC project at INFN-LNF (Frascati) have been fabricated and undergone initial testing at UCLA. The advanced aspects of the design of these devices are detailed. Final diagnosis of the tuning of the RF gun performance, including operating mode frequency and field balance, is described. The emittance compensating solenoid magnet, which is designed to be tuned in longitudinal position by differential excitation of the coils, has been measured using Hall probe scans for field profiling, and pulsed wire methods to determine the field center. Comparisons between measurements and the predictions of design codes are made.

  15. Experimental Demonstration of Emittance Compensation with Velocity Bunching

    SciTech Connect

    Ferrario, M.; Alesini, D.; Bellaveglia, M.; Boni, R.; Boscolo, M.; Castellano, M.; Chiadroni, E.; Cultrera, L.; Di Pirro, G.; Ficcadenti, L.; Filippetto, D.; Fusco, V.; Gallo, A.; Gatti, G.; Marrelli, C.; Migliorati, M.; Mostacci, A.; Pace, E.; Palumbo, L.; Spataro, B.

    2010-02-05

    In this Letter we report the first experiments aimed at the simultaneous demonstration of the emittance compensation process and velocity bunching in a high brightness electron source, the SPARC photoinjector in INFN-LNF. While a maximum compression ratio up to a factor 14 has been observed, in a particular case of interest a compression factor of 3, yielding a slice current of 120 A with less than 2 {mu}m slice emittance, has been measured. This technique may be crucial in achieving high brightness beams in photoinjectors aiming at optimized performance of short wavelength single-pass free electron lasers or other advanced applications in laser-plasma accelerators.

  16. PREFACE: Scientific Organising Committee Information

    NASA Astrophysics Data System (ADS)

    2015-06-01

    Edited by: Oliver Roberts Lorraine Hanlon Sheila McBreen Local Organising Committee: Oliver Roberts (Chair) Antonio Martin-Carrillo Lorraine Hanlon Sheila McBreen Alexey Uliyanov David Murphy Sinéad Hales Scientific Organising Committee:: Sheila McBreen, (UCD, Ireland) (Chair) Franco Camera (INFN-Milano, Italy) Nerine Cherepy (LLNL, USA) Jarek Glodo (RMD, USA) Lorraine Hanlon (UCD, Ireland) Paul Lecoq (CERN, Switzerland) Julie McEnery (NASA, USA) Oliver Roberts (UCD, Ireland) Anant Setlur, (GE, USA) Brian Shortt, (ESA, the Netherlands) Kenneth Stanton (UCD, Ireland)

  17. Study of the {sup 19}O states via the ({sup 7}Li,{sup 7}Be) reaction at 52 MeV

    SciTech Connect

    Cavallaro, M.; Cappuzzello, F.; Cunsolo, A.; Carbone, D.; Foti, A.; Lenske, H.

    2010-04-30

    The {sup 19}F({sup 7}Li,{sup 7}Be){sup 19}O reaction at 52 MeV incident energy has been performed at INFN-LNS in Catania using the MAGNEX large acceptance spectrometer. The use of an algebraic ray-reconstruction technique has allowed to extract the {sup 19}O excitation energy spectrum and the experimental angular distributions. A theoretical analysis based on the Quasi-particle Random Phase Approximation has described the transitions to the 1p-1h states of the {sup 19}O, in close agreement with the experimental cross sections without using any scaling factor.

  18. Design of a 10-bit segmented current-steering digital-to-analog converter in CMOS 65 nm technology for the bias of new generation readout chips in high radiation environment

    NASA Astrophysics Data System (ADS)

    De Robertis, G.; Loddo, F.; Mattiazzo, S.; Pacher, L.; Pantano, D.; Tamma, C.

    2016-01-01

    A new pixel front end chip for HL-LHC experiments in CMOS 65nm technology is under development by the CERN RD53 collaboration together with the Chipix65 INFN project. This work describes the design of a 10-bit segmented current-steering Digital-to-Analog Converter (DAC) to provide a programmable bias current to the analog blocks of the circuit. The main requirements are monotonicity, good linearity, limited area consumption and radiation hardness up to 10 MGy. The DAC was prototyped and electrically tested, while irradiation tests will be performed in Autumn 2015.

  19. Particle Dark Matter and DAMA/LIBRA

    SciTech Connect

    Bernabei, R.; Nozzoli, F.; Belli, P.; Cappella, F.; D'Angelo, A.; Prosperi, D.; Cerulli, R.; Dai, C. J.; He, H. L.; Ma, X. H.; Sheng, X. D.; Wang, R. G.; Montecchia, F.; Ye, Z. P.

    2010-03-26

    The DAMA/LIBRA set-up (about 250 kg highly radiopure NaI(Tl) sensitive mass) is running at the Gran Sasso National Laboratory of the I.N.F.N.. The first DAMA/LIBRA results confirm the evidence for the presence of a Dark Matter particle component in the galactic halo, as pointed out by the former DAMA/NaI set-up; cumulatively the data support such evidence at 8.2 sigma C.L. and satisfy all the many peculiarities of the Dark Matter annual modulation signature. The main aspects and prospects of this model independent experimental approach will be outlined.

  20. Simulation of particle acceleration in the PLASMONX project

    NASA Astrophysics Data System (ADS)

    Benedetti, Carlo

    2010-02-01

    In this paper I will present some numerical studies and parameter scans performed with the electromagnetic, rela-tivistic, fully-self consistent particle-in-cell (PIC) code ALaDyn (Acceleration by LAser and DYNamics of charged particles), concerning electron acceleration via plasma waves in the framework of the INFN-PLASMONX (PLASma acceleration and MONochromatic X-ray production) project. In particular I will focus on the modelling of the SITE (Self Injection Test Experiment) which will be a relevant part of the commissioning of the FLAME laser. Some issues related to the quality of the accelerated bunch will be discussed.

  1. NUMEN Project @ LNS : Heavy ions double charge exchange reactions towards the 0νββ nuclear matrix element determination

    NASA Astrophysics Data System (ADS)

    Agodi, C.; Cappuzzello, F.; Bonanno, D. L.; Bongiovanni, D. G.; Branchina, V.; Calabretta, L.; Calanna, A.; Carbone, D.; Cavallaro, M.; Colonna, M.; Cuttone, G.; Foti, A.; Finocchiaro, P.; Greco, V.; Lanzalone, G.; Lo Presti, D.; Longhitano, F.; Muoio, A.; Pandola, L.; Rifuggiato, D.; Tudisco, S.

    2015-10-01

    In the NUMEN Project it is proposed an innovative technique to access the nuclear matrix elements entering in the expression of the life-time of the neutrinoless double beta decay, using relevant cross sections of double charge exchange reactions. A key aspect is the use of MAGNEX large acceptance magnetic spectrometer, for the detection of the ejectiles, and of the INFN Laboratori Nazionali del Sud (LNS) K800 Superconducting Cyclotron (CS), for the acceleration of the required high resolution and low emittance heavy-ion beams.

  2. Reaction dynamics induced by the radioactive ion beam 7Be on medium-mass and heavy targets

    NASA Astrophysics Data System (ADS)

    Mazzocco, M.; Boiano, A.; Boiano, C.; La Commara, M.; Manea, C.; Parascandolo, C.; Pierroutsakou, D.; Stefanini, C.; Strano, E.; Torresi, D.; Acosta, L.; Di Meo, P.; Fernandez-Garcia, J. P.; Glodariu, T.; Grebosz, J.; Guglielmetti, A.; Keeley, N.; Lay, J. A.; Marquinez-Duran, G.; Martel, I.; Mazzocchi, C.; Molini, P.; Nicoletto, M.; Pakou, A.; Parkar, V. V.; Rusek, K.; Sánchez-Benítez, A. M.; Sandoli, M.; Sava, T.; Sgouros, O.; Signorini, C.; Silvestri, R.; Soramel, F.; Soukeras, V.; Stiliaris, E.; Stroe, L.; Toniolo, N.; Zerva, K.

    2015-10-01

    We studied the reaction dynamics induced at Coulomb barrier energies by the weakly-bound Radioactive Ion Beam 7Be (Sα = 1.586 MeV) on medium-mass (58Ni) and heavy (208Pb) targets. The experiments were performed at INFN-LNL (Italy), where a 2-3×105 pps 7Be secondary beam was produced with the RIB in-flight facility EXOTIC. Charged reaction products were detected by means of high-granularity silicon detectors in rather wide angular ranges. The contribution presents an up-to-date status of the data analysis and theoretical interpretation for both systems.

  3. An intrinsically safe facility for forefront research and training on nuclear technologies — An example of accelerator: the SPES cyclotron

    NASA Astrophysics Data System (ADS)

    Maggiore, M.; Lombardi, A.; Piazza, L. A. C.; Prete, G.

    2014-04-01

    The SPES project, under construction at INFN, Laboratori Nazionali di Legnaro, is a research facility for nuclear and applied physics, based on a high-current H- cyclotron with two exits. One exit will be devoted to supply an ISOL facility for the production of radioactive beams, the second one can be used as driver for the ADS system. The main characteristics of the cyclotron are described together with the main building designed to operate high-current proton beams according to radioprotection rules.

  4. Commissioning of polarized-proton and antiproton beams at Fermilab

    SciTech Connect

    Yokosawa, A.

    1988-05-04

    The author described the polarized-proton and polarized-antiproton beams up to 200 GeV/c at Fermilab. The beam line, called MP, consists of the 400-m long primary and 350-m long secondary beam line followed by 60-m long experimental hall. We discuss the characteristics of the polarized beams. The Fermilab polarization projects are designated at E-581/704 initiated and carried out by an international collaboration, Argonne (US), Fermilab (US), Kyoto-Kyushu-Hiroshima-KEK (Japan), LAPP (France), Northwestern University (US), Los Alamos Laboratory (US), Rice (US), Saclay (France), Serpukhov (USSR), INFN Trieste (Italy), and University of Texas (US).

  5. Crystallization and preliminary X-ray diffraction analysis of mouse prostaglandin F2α synthase, AKR1B3

    PubMed Central

    Takashima, Yasuhide; Hatanaka, Seika; Mizohata, Eiichi; Nagata, Nanae; Fukunishi, Yoshifumi; Matsumura, Hiroyoshi; Urade, Yoshihiro; Inoue, Tsuyoshi

    2011-01-01

    Aldo-keto reductase 1B3 (AKR1B3) catalyzes the NADPH-dependent reduction of prostaglandin H2 (PGH2), which is a common intermediate of various prostanoids, to form PGF2α. AKR1B3 also reduces PGH2 to PGD2 in the absence of NADPH. AKR1B3 produced in Escherichia coli was crystallized in complex with NADPH by the sitting-drop vapour-diffusion method. The crystal was tetragonal, belonging to space group P41212 or P43212, with unit-cell parameters a = b = 107.62, c = 120.76 Å. X-ray diffraction data were collected to 2.4 Å resolution at 100 K using a synchrotron-radiation source. PMID:22139184

  6. Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase

    SciTech Connect

    Kiyota, Eduardo; Sousa, Sylvia Morais de; Santos, Marcelo Leite dos; Costa Lima, Aline da; Menossi, Marcelo; Yunes, José Andrés; Aparicio, Ricardo

    2007-11-01

    Preliminary X-ray diffraction studies of apo maize aldose reductase at 2.0 Å resolution are reported. Maize aldose reductase (AR) is a member of the aldo-keto reductase superfamily. In contrast to human AR, maize AR seems to prefer the conversion of sorbitol into glucose. The apoenzyme was crystallized in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 47.2, b = 54.5, c = 100.6 Å and one molecule in the asymmetric unit. Synchrotron X-ray diffraction data were collected and a final resolution limit of 2.0 Å was obtained after data reduction. Phasing was carried out by an automated molecular-replacement procedure and structural refinement is currently in progress. The refined structure is expected to shed light on the functional/enzymatic mechanism and the unusual activities of maize AR.

  7. AKR1B7 Is Induced by the Farnesoid X Receptor and Metabolizes Bile Acids*

    PubMed Central

    Schmidt, Daniel R.; Schmidt, Samuel; Holmstrom, Sam R.; Makishima, Makoto; Yu, Ruth T.; Cummins, Carolyn L.; Mangelsdorf, David J.; Kliewer, Steven A.

    2011-01-01

    Although bile acids are crucial for the absorption of lipophilic nutrients in the intestine, they are cytotoxic at high concentrations and can cause liver damage and promote colorectal carcinogenesis. The farnesoid X receptor (FXR), which is activated by bile acids and abundantly expressed in enterohepatic tissues, plays a crucial role in maintaining bile acids at safe concentrations. Here, we show that FXR induces expression of Akr1b7 (aldo-keto reductase 1b7) in murine small intestine, colon, and liver by binding directly to a response element in the Akr1b7 promoter. We further show that AKR1B7 metabolizes 3-keto bile acids to 3β-hydroxy bile acids that are less toxic to cultured cells than their 3α-hydroxy precursors. These findings reveal a feed-forward, protective pathway operative in murine enterohepatic tissues wherein FXR induces AKR1B7 to detoxify bile acids. PMID:21081494

  8. Plant science. Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein.

    PubMed

    Winzer, Thilo; Kern, Marcelo; King, Andrew J; Larson, Tony R; Teodor, Roxana I; Donninger, Samantha L; Li, Yi; Dowle, Adam A; Cartwright, Jared; Bates, Rachel; Ashford, David; Thomas, Jerry; Walker, Carol; Bowser, Tim A; Graham, Ian A

    2015-07-17

    Morphinan alkaloids from the opium poppy are used for pain relief. The direction of metabolites to morphinan biosynthesis requires isomerization of (S)- to (R)-reticuline. Characterization of high-reticuline poppy mutants revealed a genetic locus, designated STORR [(S)- to (R)-reticuline] that encodes both cytochrome P450 and oxidoreductase modules, the latter belonging to the aldo-keto reductase family. Metabolite analysis of mutant alleles and heterologous expression demonstrate that the P450 module is responsible for the conversion of (S)-reticuline to 1,2-dehydroreticuline, whereas the oxidoreductase module converts 1,2-dehydroreticuline to (R)-reticuline rather than functioning as a P450 redox partner. Proteomic analysis confirmed that these two modules are contained on a single polypeptide in vivo. This modular assembly implies a selection pressure favoring substrate channeling. The fusion protein STORR may enable microbial-based morphinan production. PMID:26113639

  9. Concurrence of thyrotoxicosis and Gitelman's syndrome-associated hypokalemia-induced periodic paralysis.

    PubMed

    Imashuku, Shinsaku; Teramura-Ikeda, Tomoko; Kudo, Naoko; Kaneda, Shigehiro; Tajima, Toshihiro

    2012-04-01

    A 16-year-old Japanese boy with a history of truancy had been treated at a psychiatric clinic. When the patient was referred to us for hypokalemia-associated paralysis, the diagnosis of thyrotoxic hypokalemic periodic paralysis was made, common in Asian men. Subsequently, the patient was found to have persistently high plasma renin and aldos-terone levels. Thus, solute carrier family 12 member 3 gene (SLC12A3) analysis was performed. A novel missense homozygous mutation CTC->CAC at codon 858 (L858H) was found for which the patient was homozygous and his non-consanguineous parents heterozygote. These findings indicated that the patient developed hypokalemia-associated paralysis concurrently with thyrotoxicosis and Gitelman's syndrome. This case underscores the importance of careful examinations of adolescents with complaints of truancy as well as of precise determinations of the causes of hypokalemia-associated paralysis. PMID:22802996

  10. Selective AKR1C3 Inhibitors Potentiate Chemotherapeutic Activity in Multiple Acute Myeloid Leukemia (AML) Cell Lines.

    PubMed

    Verma, Kshitij; Zang, Tianzhu; Gupta, Nehal; Penning, Trevor M; Trippier, Paul C

    2016-08-11

    We report the design, synthesis, and evaluation of potent and selective inhibitors of aldo-keto reductase 1C3 (AKR1C3), an important enzyme in the regulatory pathway controlling proliferation, differentiation, and apoptosis in myeloid cells. Combination treatment with the nontoxic AKR1C3 inhibitors and etoposide or daunorubicin in acute myeloid leukemia cell lines, elicits a potent adjuvant effect, potentiating the cytotoxicity of etoposide by up to 6.25-fold and the cytotoxicity of daunorubicin by >10-fold. The results validate AKR1C3 inhibition as a common adjuvant target across multiple AML subtypes. These compounds in coadministration with chemotherapeutics in clinical use enhance therapeutic index and may avail chemotherapy as a treatment option to the pediatric and geriatric population currently unable to tolerate the side effects of cancer drug regimens. PMID:27563402

  11. Substrate specificity of an aflatoxin-metabolizing aldehyde reductase.

    PubMed Central

    Ellis, E M; Hayes, J D

    1995-01-01

    The enzyme from rat liver that reduces aflatoxin B1-dialdehyde exhibits a unique catalytic specificity distinct from that of other aldo-keto reductases. This enzyme, designated AFAR, displays high activity towards dicarbonyl-containing compounds with ketone groups on adjacent carbon atoms; 9,10-phenanthrenequinone, acenaphthenequinone and camphorquinone were found to be good substrates. Although AFAR can also reduce aromatic and aliphatic aldehydes such as succinic semialdehyde, it is inactive with glucose, galactose and xylose. The enzyme also exhibits low activity towards alpha,beta-unsaturated carbonyl-containing compounds. Determination of the apparent Km reveals that AFAR has highest affinity for 9,10-phenanthrenequinone and succinic semialdehyde, and low affinity for glyoxal and DL-glyceraldehyde. PMID:8526867

  12. Luna B. Leopold--pioneer setting the stage for modern hydrology

    USGS Publications Warehouse

    Hunt, Randall J.; Meine, Curt

    2012-01-01

    In 1986, during the first year of graduate school, the lead author was sampling the water from a pitcher pump in front of “The Shack,” the setting of the opening essays in Aldo Leopold's renowned book A Sand County Almanac. The sampling was part of my Master's work that included quarterly monitoring of water quality on the Leopold Memorial Reserve (LMR) near Baraboo, Wisconsin. The Shack was already a well-known landmark, and it was common to come upon visitors and hikers there. As such, I took no special note of the man who approached me as I was filling sample bottles and asked, as was typical, “What are you doing?”

  13. Yaws: 110 years after Castellani's discovery of Treponema pallidum subspecies pertenue.

    PubMed

    Stamm, Lola V

    2015-07-01

    Yaws is a neglected infectious disease that affects mostly children and adolescents living in poor, rural communities in humid, tropical areas of Africa, southeast Asia, and the Pacific Islands. The etiological agent of yaws, Treponema pallidum subspecies pertenue (T. pertenue), was discovered by Aldo Castellani in 1905 shortly after Schaudinn and Hoffmann discovered the etiological agent of syphilis, T. pallidum subspecies pallidum. The discovery of T. pertenue enabled the development of animal models and the identification of an effective antibiotic treatment (i.e., penicillin) for yaws. A World Health Organization (WHO) mass treatment campaign from 1952 to 1964 reduced the global burden of yaws by 95%, but failed to eradicate this disease. Today, 110 years after Castellani's discovery of T. pertenue, yaws is again targeted for eradication. Recent advances in the treatment and diagnosis of yaws improve the likelihood of success this time. However, several challenges must be overcome to make the goal of yaws eradication attainable. PMID:25870417

  14. The C-terminal loop of aldehyde reductase determines the substrate and inhibitor specificity.

    PubMed

    Barski, O A; Gabbay, K H; Bohren, K M

    1996-11-12

    Human aldehyde reductase has a preference for carboxyl group-containing negatively charged substrates. It belongs to the NADPH-dependent aldo-keto reductase superfamily whose members are in part distinguished by unique C-terminal loops. To probe the role of the C-terminal loops in determining substrate specificities in these enzymes, two arginine residues, Arg308 and Arg311, located in the C-terminal loop of aldehyde reductase, and not found in any other C-terminal loop, were replaced with alanine residues. The catalytic efficiency of the R311A mutant for aldehydes containing a carboxyl group is reduced 150-250-fold in comparison to that of the wild-type enzyme, while substrates not containing a negative charge are unaffected. The R311A mutant is also significantly less sensitive to inhibition by dicarboxylic acids, indicating that Arg311 interacts with one of the carboxyl groups. The inhibition pattern indicates that the other carboxyl group binds to the anion binding site formed by Tyr49, His112, and the nicotinamide moiety of NADP+. The correlation between inhibitor potency and the length of the dicarboxylic acid molecules suggests a distance of approximately 10 A between the amino group of Arg311 and the anion binding site in the aldehyde reductase molecule. The sensitivity of inhibition of the R311A mutant by several commercially available aldose reductase inhibitors (ARIs) was variable, with tolrestat and zopolrestat becoming more potent inhibitors (30- and 5-fold, respectively), while others remained the same or became less potent. The catalytic properties, substrate specificity, and susceptibility to inhibition of the R308A mutant remained similar to that of the wild-type enzyme. The data provide direct evidence for C-terminal loop participation in determining substrate and inhibitor specificity of aldo-keto reductases and specifically identifies Arg311 as the basis for the carboxyl-containing substrate preference of aldehyde reductase. PMID:8916913

  15. Interdisciplinary Adventures in Perceptual Ecology

    NASA Astrophysics Data System (ADS)

    Bocast, Christopher S.

    A portfolio dissertation that began as acoustic ecology and matured into perceptual ecology, centered on ecomusicology, bioacoustics, and translational audio-based media works with environmental perspectives. The place of music in Western eco-cosmology through time provides a basis for structuring an environmental history of human sound perception. That history suggests that music may stabilize human mental activity, and that an increased musical practice may be essential for the human project. An overview of recent antecedents preceding the emergence of acoustic ecology reveals structural foundations from 20th century culture that underpin modern sound studies. The contextual role that Aldo Leopold, Jacob von Uexkull, John Cage, Marshall McLuhan, and others played in anticipating the development of acoustic ecology as an interdiscipline is detailed. This interdisciplinary aspect of acoustic ecology is defined and defended, while new developments like soundscape ecology are addressed, though ultimately sound studies will need to embrace a broader concept of full-spectrum "sensory" or "perceptual" ecology. The bioacoustic fieldwork done on spawning sturgeon emphasized this necessity. That study yielded scientific recordings and spectrographic analyses of spawning sounds produced by lake sturgeon, Acipenser fulvescens, during reproduction in natural habitats in the Lake Winnebago watershed in Wisconsin. Recordings were made on the Wolf and Embarrass River during the 2011-2013 spawning seasons. Several specimens were dissected to investigate possible sound production mechanisms; no sonic musculature was found. Drumming sounds, ranging from 5 to 7 Hz fundamental frequency, verified the infrasonic nature of previously undocumented "sturgeon thunder". Other characteristic noises of sturgeon spawning including low-frequency rumbles and hydrodynamic sounds were identified. Intriguingly, high-frequency signals resembling electric organ discharges were discovered. These

  16. Impaired Self-Renewal and Increased Colitis and Dysplastic Lesions in Colonic Mucosa of AKR1B8 Deficient Mice

    PubMed Central

    Shen, Yi; Ma, Jun; Yan, Ruilan; Ling, Hongyan; Li, Xiaoning; Yang, Wancai; Gao, John; Huang, Chenfei; Bu, Yiwen; Cao, Yu; He, Yingchun; Wan, Laxiang; Zu, Xuyu; Liu, Jianghua; Huang, Mei Chris; Stenson, William F; Liao, Duan-Fang; Cao, Deliang

    2015-01-01

    Purpose Ulcerative colitis (UC) and colitis-associated colorectal cancer (CAC) is a serious health issue, but etiopathological factors remain unclear. Aldo-keto reductase 1B10 (AKR1B10) is specifically expressed in the colonic epithelium, but down-regulated in colorectal cancer. This study was aimed to investigate the etiopathogenic role of AKR1B10 in UC and CAC. Experimental design UC and CAC biopsies (paraffin-embedded sections) and frozen tissues were collected to examine AKR1B10 expression. Aldo-keto reductase 1B8 (the ortholog of human AKR1B10) knockout (AKR1B8 −/−) mice were produced to estimate its role in the susceptibility and severity of chronic colitis and associated dysplastic lesions, induced by dextran sulfate sodium (DSS) at a low dose (2%). Genome-wide Exome sequencing was used to profile DNA damage in DSS-induced colitis and tumors. Results AKR1B10 expression was markedly diminished in over 90% of UC and CAC tissues. AKR1B8 deficiency led to reduced lipid synthesis from butyrate and diminished proliferation of colonic epithelial cells. The DSS-treated AKR1B8 −/− mice demonstrated impaired injury repair of colonic epithelium and more severe bleeding, inflammation, and ulceration. These AKR1B8 −/− mice had more severe oxidative stress and DNA damage, and dysplasias were more frequent and at a higher grade in the AKR1B8 −/− mice than in wild type mice. Palpable masses were seen in the AKR1B8 −/− mice only, not in wild type. Conclusion AKR1B8 is a critical protein in the proliferation and injury repair of the colonic epithelium and in the pathogenesis of UC and CAC, being a new etiopathogenic factor of these diseases. PMID:25538260

  17. Central angiotensin II induces thirst-related responses in an amphibian.

    PubMed

    Propper, C R; Hillyard, S D; Johnson, W E

    1995-03-01

    Angiotensin II (A-II), a potent inducer of thirst-related behavior in many vertebrate species, was injected into the third ventricle of the brain of the spadefoot toad, Scaphiopus couchii. Following injection of 10 ng A-II the animals demonstrated a significant increase in water absorption response (WR) behavior, in which toads press their ventral skin to a moist surface and absorb water by osmosis. This increase in the frequency of WR behavior was positively correlated with an increase in water gain during a 2-hr period indicating that centrally injected A-II stimulates water intake by this amphibian species. We have previously demonstrated that WR behavior is also induced by intraperitoneal (i.p.) injections of A-II in several anuran species, including S. couchii. Thus, amphibians, like other vertebrates, demonstrate an increase in water intake in response to either centrally administered or circulating A-II. A second series of experiments was conducted to determine whether the above response to A-II might be secondary to increases in the circulating levels of aldosterone (ALDO) or antidiuretic hormone because the release of both of these hormones has been shown by others to be stimulated by A-II. Scaphiopus couchii injected i.p. with either ALDO or arginine vasotocin in dosages of 1, 10, and 100 micrograms/100 g body weight showed no increase in WR behavior relative to toads injected with saline alone. These results suggest that A-II acts directly on the brain of S. couchii to induce WR behavior. PMID:7782064

  18. Structural analysis of the catalytic mechanism and stereoselectivity in Streptomyces coelicolor alditol oxidase.

    PubMed

    Forneris, Federico; Heuts, Dominic P H M; Delvecchio, Manuela; Rovida, Stefano; Fraaije, Marco W; Mattevi, Andrea

    2008-01-22

    Alditol oxidase (AldO) from Streptomyces coelicolor A3(2) is a soluble monomeric flavin-dependent oxidase that performs selective oxidation of the terminal primary hydroxyl group of several alditols. Here, we report the crystal structure of the recombinant enzyme in its native state and in complex with both six-carbon (mannitol and sorbitol) and five-carbon substrates (xylitol). AldO shares the same folding topology of the members of the vanillyl-alcohol oxidase family of flavoenzymes and exhibits a covalently linked FAD which is located at the bottom of a funnel-shaped pocket that forms the active site. The high resolution of the three-dimensional structures highlights a well-defined hydrogen-bonding network that tightly constrains the substrate in the productive conformation for catalysis. Substrate binding occurs through a lock-and-key mechanism and does not induce conformational changes with respect to the ligand-free protein. A network of charged residues is proposed to favor catalysis through stabilization of the deprotonated form of the substrate. A His side chain acts as back door that "pushes" the substrate-reactive carbon atom toward the N5-C4a locus of the flavin. Analysis of the three-dimensional structure reveals possible pathways for diffusion of molecular oxygen and a small cavity on the re side of the flavin that may host oxygen during FAD reoxidation. These features combined with the tight shape of the catalytic site provide insights into the mechanism of AldO-mediated regioselective oxidation reactions and its substrate specificity. PMID:18154360

  19. Integrated thermal-optical simulations of a GNSS Retroreflector Array

    NASA Astrophysics Data System (ADS)

    Boni, A.; Cantone, C.; Dell'Agnello, S.; Ciocci, E.; Contessa, S.; Delle Monache, G. O.; Lops, C.; Maiello, M.; Martini, M.; Mondaini, C.; Patrizi, G.; Porcelli, L.; Salvatori, L.; Tibuzzi, M.; Tuscano, P.; Vittori, R.; Bianco, G.

    2015-07-01

    Satellite Laser Ranging (SLR) is one of the most precise and cost effective satellite positioning techniques, used for many years on geodetic, Earth observation and scientific satellites. In the last decades it has been applied on radio-navigation satellites as well, however still in a limited way. The benefits that such technique could bring to these satellites are many, but, in order to support at best their tracking, some improvements need to be done; one is the enhancement of optical response of SLR payloads throughout the orbit. INFN-LNF with the ASI-INFN project ETRUSCO-2 studied hardware and software solution to optimize the optical response of such payloads, with the design and fabrication of a GNSS Retroreflector Array (GRA). We simulated the performance of a model of the GRA in a specific orbit of Galileo, showing contained variation of optical intensity, approximately 5%, throughout the orbit. A comparison between two polarization states, linear and circular, proves how a circularly polarized beam could reduce, up to 3 times, intensity fluctuations at a fixed velocity aberration.

  20. The Experimental Discovery of Double-Charm Baryons

    NASA Astrophysics Data System (ADS)

    Engelfried, Jürgen; Selex Collaboration

    2005-04-01

    In 2002, the SELEX [The SELEX (Fermilab E781) Collaboration: Ball State University, Bogazici University, Carnegie-Mellon University, Centro Brasileiro de Pesquisas Fisicas, Fermilab, Institute For High Energy Physics (Protvino), Institute of High Energy Physics (Beijing), Institute of Theoretical and Experimental Physics (Moscow), Max-Planck-Institute for Nuclear Physics, Moscow State University, Petersburg Nuclear Physics Institute, Tel Aviv University, Universidad Autónoma de San Luis Potosí, Universidade Federal da Paraíba, H. H. Wills Physics Laboratory, University of Bristol, University of Iowa, University of Michigan-Flint, University of Rochester, University of Rome La Sapienza and INFN, University of São Paulo, University of Trieste and INFN. http://www-selex.fnal.gov] Experiment (Fermilab E781) reported the first observation of a member of the family of doubly charmed baryons [SELEX Collaboration, M. Mattson et al.: First observation of the doubly charmed baryonΞcc+. Phys. Rev. Letters 89 (2002) 112001, [ arXiv:hep-ex/0208014

  1. Strip Ionization Chamber as Beam Monitor in the Proton Therapy Eye Treatment

    NASA Astrophysics Data System (ADS)

    Marchetto, F.; Cirio, R.; Garella, M. A.; Giordanengo, S.; Boriano, A.; Givehchi, N.; La Rosa, A.; Peroni, C.; Donetti, M.; Bourhaleb, F.; Pitta', G.; Cirrone, G. A. P.; Cuttone, G.; Raffaele, L.; Sabini, M. G.; Valastro, L.

    2006-04-01

    Since spring 2002, ocular pathologies have been treated in Catania at the Centro di AdroTerapia e Applicazioni Nucleari Avanzate (CATANA) within a collaboration between INFN Laboratori Nazionali del Sud (LNS), Physics Department, Ophthalmology Institute, Radiology Institute of the Catania University and CSFNSM Catania. A beam line from a 62 MeV Superconducting Cyclotron is used to treat shallow tumors. The beam is conformed to the tumor shape with a passive delivery system. A detector system has been developed in collaboration with INFN-Torino to be used as real time beam monitor. The detector, placed upstream of the patient collimator, consists of two parallel plate ionization chambers with the anode segmented in strips. Each anode is made of 0.5 mm-wide 256 strips corresponding to (12.8 × 12.8) cm2 sensitive area. With the two strip ionization chambers one can measure the relevant beam parameters during treatment to probe both asymmetry and flatness. In the test carried out at CATANA the detector has been used under different and extreme beam conditions. Preliminary results are given for profiles and skewness, together with a comparison with reference detectors.

  2. Ther FERMI FEL project at TRIESTE

    SciTech Connect

    Walker, R.P.; Bulfone, D.; Cargnello, F.

    1995-12-31

    The goal of the FERMI project - Free Electron Radiation and Matching Instrumentation - is to construct a new user facility for FEL radiation beams covering a broad spectral range (2-250 {mu}m) to complement the high brightness VUV/Soft-Xray radiation available from the ELETTRA synchrotron radiation facility at Trieste. A unique feature of the project will be the possibility of carrying out {open_quote}pump-probe{close_quote} experiments using synchronized radiation beams from FERMI and ELETTRA on the same sample. The project was launched at a meeting with Italian FEL experts held in Trieste on the 18th November 1994, chaired by C. Rubbia, as a collaboration between Sincrotrone Trieste, ENEA (Frascati), INFN (Frascati) and the University of Naples (Department of Electronic Engineering). The facility will make use of an existing linac, that forms part of the ELETTRA injection system, and a hall into which the beam can be extracted. In addition, for the first phase of the project equipment will be used from the suspended INFN/ENEA {open_quote}SURF{close_quote} FEL experiment, including the undulator, beam transport magnets and optical cavity. In this first International FEL Conference report on the project, we summarize the main features of the project, concentrating in particular on the most recent activities, including: results of measurements of the linac beam in the FEL mode of operation, further studies of the electron beam transport system including possibilities for bunch length manipulations, and further numerical calculations of the FEL performance.

  3. The computing and data infrastructure to interconnect EEE stations

    NASA Astrophysics Data System (ADS)

    Noferini, F.

    2016-07-01

    The Extreme Energy Event (EEE) experiment is devoted to the search of high energy cosmic rays through a network of telescopes installed in about 50 high schools distributed throughout the Italian territory. This project requires a peculiar data management infrastructure to collect data registered in stations very far from each other and to allow a coordinated analysis. Such an infrastructure is realized at INFN-CNAF, which operates a Cloud facility based on the OpenStack opensource Cloud framework and provides Infrastructure as a Service (IaaS) for its users. In 2014 EEE started to use it for collecting, monitoring and reconstructing the data acquired in all the EEE stations. For the synchronization between the stations and the INFN-CNAF infrastructure we used BitTorrent Sync, a free peer-to-peer software designed to optimize data syncronization between distributed nodes. All data folders are syncronized with the central repository in real time to allow an immediate reconstruction of the data and their publication in a monitoring webpage. We present the architecture and the functionalities of this data management system that provides a flexible environment for the specific needs of the EEE project.

  4. New markers to identify the provenance of lapis lazuli: trace elements in pyrite by means of micro-PIXE

    NASA Astrophysics Data System (ADS)

    Re, A.; Angelici, D.; Lo Giudice, A.; Maupas, E.; Giuntini, L.; Calusi, S.; Gelli, N.; Massi, M.; Borghi, A.; Gallo, L. M.; Pratesi, G.; Mandò, P. A.

    2013-04-01

    Lapis lazuli has been used for glyptics and carving since the fifth millennium BC to produce jewels, amulets, seals, inlays, etc; the identification of the origin of the stone used for carving artworks may be valuable for reconstructing old trade routes. Since ancient lapis lazuli art objects are precious, only non-destructive techniques can be used to identify their provenance, and ion beam analysis (IBA) techniques allow us to characterise this stone in a fully non-invasive way. In addition, by using an ion microprobe, we have been able to focus the analysis on single crystals, as their typical dimensions may range from a few microns to hundreds of microns. Provenance markers, identified in previous IBA studies and already presented elsewhere, were based on the presence/absence of mineral phases, on the presence/quantity of trace elements inside a phase and on characteristic features of the luminescence spectra. In this work, a systematic study on pyrite crystals, a common accessory mineral in lapis lazuli, was carried out, following a multi-technique approach: optical microscopy and SEM-EDX to select crystals for successive trace element micro-PIXE measurements at two Italian facilities, the INFN Laboratori Nazionali di Legnaro and the INFN LABEC laboratory in Firenze. The results of this work allowed us to obtain new markers for lapis lazuli provenance identification.

  5. Effect of advanced nanowire-based targets in nanosecond laser-matter interaction (invited)

    NASA Astrophysics Data System (ADS)

    Lanzalone, G.; Altana, C.; Mascali, D.; Muoio, A.; Malferrari, L.; Odorici, F.; Malandrino, G.; Tudisco, S.

    2016-02-01

    An experimental campaign aiming to investigate the effects of innovative nanostructured targets based on Ag nanowires on laser energy absorption in the ns time domain has been carried out at the Laser Energy for Nuclear Science laboratory of INFN-LNS in Catania. The tested targets were realized at INFN-Bologna by anodizing aluminium sheets in order to obtain layers of porous Al2O3 of different thicknesses, on which nanowires of various metals are grown by electro-deposition with different heights. Targets were then irradiated by using a Nd:YAG laser at different pumping energies. Advanced diagnostic tools were used for characterizing the plasma plume and ion production. As compared with targets of pure Al, a huge enhancement (of almost two order of magnitude) of the X-ray flux emitted by the plasma has been observed when using the nanostructured targets, with a corresponding decrease of the "optical range" signal, pointing out that the energetic content of the laser produced plasma was remarkably increased. This analysis was furthermore confirmed from time-of-flight spectra.

  6. Design of the ELIMAIA ion collection system

    NASA Astrophysics Data System (ADS)

    Schillaci, F.; Cirrone, G. A. P.; Cuttone, G.; Maggiore, M.; Andó, L.; Amato, A.; Costa, M.; Gallo, G.; Korn, G.; Larosa, G.; Leanza, R.; Manna, R.; Margarone, D.; Milluzzo, G.; Pulvirenti, S.; Romano, F.; Salamone, S.; Sedita, M.; Scuderi, V.; Tramontana, A.

    2015-12-01

    A system of permanent magnet quadrupoles (PMQs) is going to be realized by INFN-LNS to be used as a collection system for the injection of laser driven ion beams up to 60 MeV/u in an energy selector based on four resistive dipoles. This system is the first element of the ELIMED (ELI-Beamlines MEDical and Multidisciplinary applications) beam transport, dosimetry and irradiation line that will be developed by INFN-LNS (It) and installed at the ELI-Beamlines facility in Prague (Cz). ELIMED will be the first user's open transport beam-line where a controlled laser-driven ion beam will be used for multidisciplinary researches. The definition of well specified characteristics, both in terms of performances and field quality, of the magnetic lenses is crucial for the system realization, for the accurate study of the beam dynamics and for the proper matching with the magnetic selection system which will be designed in the next months. Here, we report the design of the collection system and the adopted solutions in order to realize a robust system form the magnetic point of view. Moreover, the first preliminary transport simulations are also described.

  7. Editorial

    NASA Astrophysics Data System (ADS)

    Bruzzi, Mara; Pace, Emanuele; Talamonti, Cinzia

    2013-12-01

    The 9th edition of the International Conference on Radiation Effects on Semiconductor Materials, Detectors and Devices (RESMDD), held in Florence, at Dipartimento di Fisica ed Astronomia on October 9-12, 2012, was aimed at discussing frontier research activities in several application fields as in nuclear and particle physics, astrophysics, medical and solid-state physics. Main topics discussed in this conference are tracking performance of heavily irradiated silicon detectors, developments required for the luminosity upgrade of the Large Hadron Collider (HL-LHC), radiation effects on semiconductor materials for medical (radiotherapy dosimeters, imaging devices), astrophysics (UV, X- and γ-ray detectors) and environmental applications, microscopic defect analysis of irradiated semiconductor materials and related radiation hardening technologies. On the first day the conference hosted a short course intended to introduce fundamentals in the development of semiconductor detectors for medical applications to graduate and PhD students, post-docs and young researchers, both engineers and physicists. Directors of the School were Prof. Marta Bucciolini of the University of Florence and INFN, Italy and Dr. Carlo Civinini, INFN Firenze, Italy. Emphasis was placed on the underlying physical principles, instrument design, factors affecting performance, and applications in both the clinical and preclinical applications. The School was attended by nearly 40 students/ young researchers. We warmly thank the Directors for organizing this interesting event and the professors and researchers who gave lessons, for sharing their experience and knowledge with the students.

  8. FOREWORD: TAUP 2005: Proceedings of the Ninth International Conference on Topics in Astroparticle and Underground Physics

    NASA Astrophysics Data System (ADS)

    Bottino, Alessandro; Coccia, Eugenio; Morales, Julio; Puimedónv, Jorge

    2006-04-01

    The ninth meeting of the TAUP Workshop Series, TAUP 2005, was organized by the University of Zaragoza and Laboratorio Subterráneo de Canfranc, jointly with the Laboratori Nazionali del Gran Sasso of the Italian Institute of Nuclear Physics (INFN). It was dedicated to the memory of professor Angel Morales, co-founder of the TAUP Series and a central figure in the scientific shaping and organization of the TAUP conferences since their inception in 1989. He and his group of collaborators laid, twenty years ago, the foundations of underground physics in Spain. To have TAUP 2005 hosted by the University of Zaragoza was a tangible way of honouring his memory. The Conference was concluded by a visit to the new installations of the Canfranc Laboratory, where a memorial ceremony was held in honour of Angel Morales, the driving force for the creation of that Laboratory. In TAUP 2005 all the various aspects of Astroparticle Physics have been covered, from Cosmology and Dark Constituents, to Gravitational Waves, to Neutrino Physics and Astrophysics, to High Energy Astrophysics, to Cosmic Rays and Gamma-Rays Astronomy. New and important scientific results were presented and debated in the plenary review talks and in a very large number of contributions in topical parallel sessions. As editors of these proceedings, we hope that this volume, which contains most of the talks and contributions presented at TAUP 2005, will provide a detailed state-of-the-art account of the various facets of Astroparticle Physics. We thank all the invited speakers and contributors who made this possible. Full coverage of the transparencies presented at the conference can be found on the website http://www.unizar.es/taup2005. At TAUP 2005 a memorial lecture was delivered by Art McDonald to commemorate John Bahcall, who passed away prematurely in August 2005. In this talk, his figure, as a pioneer and leader in the fields of Neutrino Physics, Astronomy and Astrophysics and as a man of great personal

  9. A new monitoring microsystem for radiation measurement based on VSLI technique

    NASA Astrophysics Data System (ADS)

    Cai, G.; Cerullo, N.; Martini, E.; Rancoita, P. G.; Riscossa, P.

    1998-02-01

    During the researches performed at CERN in high energy physics a new semiconductor gamma detector, based on VSLI electronics was developed at INFN. This device is little, cheap reliable and presents high performances. In the frame of a collaboration work, performed between DITEC, Ansaldo Ricerche, and INFN, was proposed, as possible, a new use of this detector, different from high energy experiments, namely, the "AVERAGE AXIAL POWER DISTRIBUTION MEASUREMENT IN A NUCLEAR FISSION REACTOR, USING MANY EXCORE DETECTORS". At present, the current PWR Ex-Core Nuclear Instrumentation System (NIS), is based on a detection facility, composed of four/six axial ionization chambers, each one of which monitors the ex-core neutron flux at one specific elevation. The new proposed Ex-core NIS concepts, is based on the presence of a large number of puntiform silicon detectors axially and radially arranged out of the Reactor Vessel and the concrete primary shield. The presence of a large number of detectors and the use of a fast charge preamplifier, connected to the silicon detectors (both of low cost), allows the use of a computer for on line direct control and protection system enhancing safety and economic characteristics of the nuclear reactor. In this paper, after description of the semiconductor detector, of the preamplifier, and of their performances, the above indicated application are described, stressing the safety and economics advantages to be reached through its use.

  10. A new monitoring microsystem for radiation measurement based on VSLI technique

    NASA Astrophysics Data System (ADS)

    Cai, G.; Cerullo, N.; Martini, E.; Rancoita, P. G.; Riscossa, P.

    1997-02-01

    During the researches performed at CERN in high energy physics a new semiconductor gamma detector, based on VSLI electronics was developed at INFN. This device is little, cheap reliable and presents high performances. In the frame of a collaboration work, performed between DITEC, Ansaldo Ricerche, and INFN, was proposed, as possible, a new use of this detector, different from high energy experiments ,namely, the ``AVERAGE AXIAL POWER DISTRIBUTION MEASUREMENT IN A NUCLEAR FISSION REACTOR, USING MANY EX-CORE DETECTORS''. At present, the current PWR Ex-Core Nuclear Instrumentation System (NIS), is based on a detection facility, composed of four/six axial ionization chambas, each one of which monitors the ex-core neutron flux at one specific elevation. The new proposed Ex-core NIS concepts, is based on the presence of a large number of puntiform silicon detectors axially and radially arranged out of the Reactor Vessel and the concrete primary shield. The presence of a large number of detectors and the use of a fast charge preamplifier, connected to the silicon detectors (both of low cost), allows the use of a computer for on line direct control and protection system enhancing safety and economic characteristics of the nuclear reactor. In this paper, after description of the semiconductor detector, of the preamplifier, and of their performances, the above indicated application are described, stressing the safety and economics advantages to be reached through its use.

  11. Workplace testing of the new single sphere neutron spectrometer based on Dysprosium activation foils (Dy-SSS)

    NASA Astrophysics Data System (ADS)

    Bedogni, R.; Gómez-Ros, J. M.; Esposito, A.; Gentile, A.; Chiti, M.; Palacios-Pérez, L.; Angelone, M.; Tana, L.

    2012-08-01

    A photon insensitive passive neutron spectrometer consisting of a single moderating polyethylene sphere with Dysprosium activation foils arranged along three perpendicular axes was designed by CIEMAT and INFN. The device is called Dy-SSS (Dy foil-based Single Sphere Spectrometer). It shows nearly isotropic response in terms of neutron fluence up to 20 MeV. The first prototype, previously calibrated with 14 MeV neutrons, has been recently tested in workplaces having different energy and directional distributions. These are a 2.5 MeV nearly mono-chromatic and mono-directional beam available at the ENEA Frascati Neutron Generator (FNG) and the photo-neutron field produced in a 15 MV Varian CLINAC DHX medical accelerator, located in the Ospedale S. Chiara (Pisa). Both neutron spectra are known through measurements with a Bonner Sphere Spectrometer. In both cases the experimental response of the Dy-SSS agrees with the reference data. Moreover, it is demonstrated that the spectrometric capability of the new device are independent from the directional distribution of the neutron field. This opens the way to a new generation of moderation-based neutron instruments, presenting all advantages of the Bonner sphere spectrometer without the disadvantage of the repeated exposures. This concept is being developed within the NESCOFI@BTF project of INFN (Commissione Scientifica Nazionale 5).

  12. Radiation quality and ion-beam therapy: understanding the users' needs.

    PubMed

    Magrin, G; Mayer, R; Verona, C; Grevillot, Loïc

    2015-09-01

    Ion-beam therapy faces a growing demand of tools able to map radiation quality within the irradiated volume. Although analytical computations and simulations provide useful estimations of dose and radiation quality, the direct measure of those parameters would improve ion-beam therapy in particular when deep-seated tumours are irradiated, tissue composition and density are variable or organs at risk are near the tumour. Several ion-beam therapy facilities are studying detectors and procedures for measuring the radiation quality on a microdosimetric as well as a nanodosimetric scale. Simplicity and miniaturisation of the devices are essential for measurements first in phantoms and thereafter during therapy, particularly for intra-cavity detectors. MedAustron is studying solid-state detectors based on a single crystal chemical vapour deposition diamond. In collaboration with Italian National Institute for Nuclear Physics (INFN), Tor Vergata and Legnaro; INFN-microdosimetry and track structure project; Austrian Institute of Technology, Vienna; and Italian National agency for new technologies, energy and sustainable economic development, Rome, prototypes have been developed to characterise radiation quality in sizes equivalent to one micrometre of biological tissue. PMID:26180078

  13. Measurement of neutron spectra generated by a 62 AMeV carbon-ion beam on a PMMA phantom using extended range Bonner sphere spectrometers

    NASA Astrophysics Data System (ADS)

    Bedogni, R.; Amgarou, K.; Domingo, C.; Russo, S.; Cirrone, G. A. P.; Pelliccioni, M.; Esposito, A.; Pola, A.; Introini, M. V.; Gentile, A.

    2012-07-01

    Neutrons constitute an important component of the radiation environment in hadron therapy accelerators. Their energy distribution may span from thermal up to hundred of MeV. The characterization of these fields in terms of dosimetric or spectrometric quantities is crucial for either the patient protection or the facility design aspects. To date, the Extended Range Bonner Sphere Spectrometer (ERBSS) is the only instrument able to simultaneously determine all spectral components in such workplaces. With the aim of providing useful data to the scientific community involved in neutron measurements at hadron therapy facilities, a measurement campaign was carried out at the Centro di AdroTerapia e Applicazioni Nucleari Avanzate (CATANA) of INFN-LNS (Laboratori Nazionali del Sud), where a 62 AMeV carbon ion is available. The beam was directed towards a PMMA phantom, simulating the patient, and two neutron measurement points were established at 0° and 90° with respect to the beam-line. The ERBSSs of UAB (Universidad Autónoma de Barcelona-Grup de Física de les Radiacions) and INFN (Istituto Nazionale di Fisica Nucleare-Laboratori Nazionali di Frascati) were used to measure the resulting neutron fields. The two ERBSSs use different detectors and sphere diameters, and have been independently calibrated. The FRUIT code was used to unfold the results.

  14. Effect of advanced nanowire-based targets in nanosecond laser-matter interaction (invited).

    PubMed

    Lanzalone, G; Altana, C; Mascali, D; Muoio, A; Malferrari, L; Odorici, F; Malandrino, G; Tudisco, S

    2016-02-01

    An experimental campaign aiming to investigate the effects of innovative nanostructured targets based on Ag nanowires on laser energy absorption in the ns time domain has been carried out at the Laser Energy for Nuclear Science laboratory of INFN-LNS in Catania. The tested targets were realized at INFN-Bologna by anodizing aluminium sheets in order to obtain layers of porous Al2O3 of different thicknesses, on which nanowires of various metals are grown by electro-deposition with different heights. Targets were then irradiated by using a Nd:YAG laser at different pumping energies. Advanced diagnostic tools were used for characterizing the plasma plume and ion production. As compared with targets of pure Al, a huge enhancement (of almost two order of magnitude) of the X-ray flux emitted by the plasma has been observed when using the nanostructured targets, with a corresponding decrease of the "optical range" signal, pointing out that the energetic content of the laser produced plasma was remarkably increased. This analysis was furthermore confirmed from time-of-flight spectra. PMID:26932052

  15. Study of a solid state microdosemeter based on a monolithic silicon telescope: irradiations with low-energy neutrons and direct comparison with a cylindrical TEPC.

    PubMed

    Agosteo, S; Colautti, P; Fanton, I; Fazzi, A; Introini, M V; Moro, D; Pola, A; Varoli, V

    2011-02-01

    A silicon device based on the monolithic silicon telescope technology coupled to a tissue-equivalent converter was proposed and investigated for solid state microdosimetry. The detector is constituted by a ΔE stage about 2 µm in thickness geometrically segmented in a matrix of micrometric diodes and a residual-energy measurement stage E about 500 µm in thickness. Each thin diode has a cylindrical sensitive volume 9 µm in nominal diameter, similar to that of a cylindrical tissue-equivalent proportional counter (TEPC). The silicon device and a cylindrical TEPC were irradiated in the same experimental conditions with quasi-monoenergetic neutrons of energy between 0.64 and 2.3 MeV at the INFN-Legnaro National Laboratories (LNL-INFN, Legnaro, Italy). The aim was to study the capability of the silicon-based system of reproducing microdosimetric spectra similar to those measured by a reference microdosemeter. The TEPC was set in order to simulate a tissue site about 2 μm in diameter. The spectra of the energy imparted to the ▵E stage of the silicon telescope were corrected for tissue-equivalence through an optimized procedure that exploits the information from the residual energy measurement stage E. A geometrical correction based on parametric criteria for shape-equivalence was also applied. The agreement between the dose distributions of lineal energy and the corresponding mean values is satisfactory at each neutron energy considered. PMID:21147791

  16. The data operation centre tool. Architecture and population strategies

    NASA Astrophysics Data System (ADS)

    Dal Pra, Stefano; Crescente, Alberto

    2012-12-01

    Keeping track of the layout of the informatic resources in a big datacenter is a complex task. DOCET is a database-based webtool designed and implemented at INFN. It aims at providing a uniform interface to manage and retrieve needed information about one or more datacenter, such as available hardware, software and their status. Having a suitable application is however useless until most of the information about the centre are not inserted in the DOCET'S database. Manually inserting all the information from scratch is an unfeasible task. After describing DOCET'S high level architecture, its main features and current development track, we present and discuss the work done to populate the DOCET database for the INFN-T1 site by retrieving information from a heterogenous variety of authoritative sources, such as DNS, DHCP, Quattor host profiles, etc. We then describe the work being done to integrate DOCET with some common management operation, such as adding a newly installed host to DHCP and DNS, or creating a suitable Quattor profile template for it.

  17. Design, construction and tests of a 3 GHz proton linac booster (LIBO) for cancer therapy

    NASA Astrophysics Data System (ADS)

    Berra, Paolo

    2007-12-01

    In the last ten years the use of proton beams in radiation therapy has become a clinical tool for treatment of deep-seated tumours. LIBO is a RF compact and low cost proton linear accelerator (SCL type) for hadrontherapy. It is conceived by TERA Foundation as a 3 GHz Linac Booster, to be mounted downstream of an existing cyclotron in order to boost the energy of the proton beam up to 200 MeV, needed for deep treatment (~25 cm) in the human body. With this solution it is possible to transform a low energy commercial cyclotron, normally used for eye melanoma therapy, isotope production and nuclear physics research, into an accelerator for deep-seated tumours. A prototype module of LIBO has been built and successfully tested with full RF power at CERN and with proton beam at INFN Laboratori Nazionali del Sud (LNS) in Catania, within an international collaboration between TERA Foundation, CERN, the Universities and INFN groups of Milan and Naples. The mid-term aim of the project is the technology transfer of the accumulated know-how to a consortium of companies and to bring this novel medical tool to hospitals. The design, construction and tests of the LIBO prototype are described in detail.

  18. Sperm whale assessment in the Western Ionian Sea using acoustic data from deep sea observatories

    NASA Astrophysics Data System (ADS)

    Caruso, Francesco; Bellia, Giorgio; Beranzoli, Laura; De Domenico, Emilio; Larosa, Giuseppina; Marinaro, Giuditta; Papale, Elena; Pavan, Gianni; Pellegrino, Carmelo; Pulvirenti, Sara; Riccobene, Giorgio; Scandura, Danila; Sciacca, Virginia; Viola, Salvatore

    2015-04-01

    The Italian National Institute of Nuclear Physics (INFN) operates two deep sea infrastructures: Capo Passero, Western Ionian Sea 3,600 meters of depth, and Catania Wester Ionian Sea 2,100 m depth. At the two sites, several research observatories have been run: OnDE, NEMO-SN1, SMO, KM3NeT-Italia most of them jointly operated between INFN and INGV. In all these observatories, passive acoustic sensors (hydrophones) have been installed. Passive Acoustics Monitoring (PAM) is nowadays the main tool of the bioacoustics to study marine mammals. In particular, receiving the sounds emitted by cetaceans from a multi-hydrophones array installed in a cabled seafloor observatory, a research about the ecological dynamics of the species may be performed. Data acquired with the hydrophones installed aboard the OnDE, SMO and KM3NeT-Italia observatories will be reported. Thanks to acquired data, the acoustic presence of the sperm whales was assessed and studied for several years (2005:2013). An "ad hoc" algorithm was also developed to allow the automatic identification of the "clicks" emitted by the sperm whales and measure the size of detected animals. According to the results obtained, the sperm whale population in the area is well-distributed in size, sex and sexual maturity. Although specimens more than 14 meters of length (old males) seem to be absent.

  19. MoonLIGHT: A USA-Italy lunar laser ranging retroreflector array for the 21st century

    NASA Astrophysics Data System (ADS)

    Martini, M.; Dell'Agnello, S.; Currie, D.; Delle Monache, G.; Vittori, R.; Chandler, J. F.; Cantone, C.; Boni, A.; Berardi, S.; Patrizi, G.; Maiello, M.; Garattini, M.; Lops, C.; March, R.; Bellettini, G.; Tauraso, R.; Intaglietta, N.; Tibuzzi, M.; Murphy, T. W.; Bianco, G.; Ciocci, E.

    2012-12-01

    Since the 1970s Lunar Laser Ranging (LLR) to the Apollo Cube Corner Retroreflector (CCR) arrays (developed by the University of Maryland, UMD) have supplied significant tests of General Relativity: possible changes in the gravitational constant, gravitational self-energy, weak equivalence principle, geodetic precession, inverse-square force-law. LLR has also provided significant information on the composition and origin of the Moon. This is the only Apollo experiment still in operation. In the 1970s Apollo LLR arrays contributed a negligible fraction of the ranging error budget. Since the ranging capabilities of ground stations improved by more than two orders of magnitude, now, because of the lunar librations, Apollo CCR arrays dominate the error budget. With the project MoonLIGHT (Moon Laser Instrumentation for General relativity High-accuracy Tests), in 2006 INFN-LNF joined UMD in the development and test of a new-generation LLR payload made by a single, large CCR (100 mm diameter) unaffected by librations. In particular, INFN-LNF built and is operating a new experimental apparatus (Satellite/lunar laser ranging Characterization Facility, SCF) and created a new industry-standard test procedure (SCF-Test) to characterize and model the detailed thermal behavior and the optical performance of CCRs in laboratory-simulated space conditions, for industrial and scientific applications. Our key experimental innovation is the concurrent measurement and modeling of the optical Far Field Diffraction Pattern (FFDP) and the temperature distribution of retroreflector payloads under thermal conditions produced with a solar simulator. The apparatus includes infrared cameras for non-invasive thermometry, thermal control and real-time payload movement to simulate satellite orientation on orbit with respect to solar illumination and laser interrogation beams. These capabilities provide: unique pre-launch performance validation of the space segment of LLR/SLR (Satellite Laser

  20. Extreme QCD 2012 (xQCD)

    NASA Astrophysics Data System (ADS)

    2013-04-01

    Acknowledgements This conference would not have been possible without the generous support from Brookhaven National Laboratory and the George Washington University INS and IMPACT institutes, Columbian College of Arts and Sciences, the Office of Vice-President for Research, and the Department of Physics. We thank them wholeheartedly. We are also very grateful for the support of our colleagues on the local organizing committee, Walter Freeman and Frank Lee, and on the International Advisory Committee: Simon Hands, Tetsuo Hatsuda, Frithjof Karsch, Maria Paola Lombardo, Tereza Mendes, Atsushi Nakamura, Owe Philipsen, Claudia Ratti, Paul Romatschke, Misha Stephanov, and Nu Xu. List of participants Alexandru, Andrei George Washington University Bazavov, Alexei Brookhaven National Laboratory Bloch, Jacques University of Regensburg Braun-Munzinger, Peter EMMI, GSI Breto Rangel, Guillermo CMS/UC Davis D'Elia, Massimo University of Pisa, INFN Dexheimer, Veronica UFSC - Federal University of Santa Catarina Ding, Heng-Tong Brookhaven National Laboratory Dion, Alan Stony Brook University Dumitru, Adrian RBRC and Baruch College, CUNY Freeman, Walter George Washington University Gavai, Rajiv Tata Institute (TIFR), Mumbai Hanada, Masanori KEK Theory Center Hands, Simon Swansea University Hegde, Prasad Brookhaven National Laboratory Heinke, Craig University of Alberta Horvath, Ivan University of Kentucky Karsch, Frithjof Brookhaven National Laboratory Krieg, Stefan Wuppertal University Lattimer, James Stony Brook University Lee, Frank George Washington University Li, Anyi Institute for Nuclear Theory Liu, Keh-Fei University of Kentucky Lombardo, Maria Paola INFN - LNF Lottini, Stefano Goethe University, Frankfurt am Main Maezawa, Yu Brookhaven National Laboratory Miura, Kohtaroh Laboratori Nazionali di Frascati - INFN Monnai, Akihiko The University of Tokyo Mukherjee, Swagato Brookhaven National Laboratory Myers, Joyce University of Groningen Nakamura, Atsushi RIISE, Hiroshima

  1. Changes in urinary excretion of water and sodium transporters during amiloride and bendroflumethiazide treatment

    PubMed Central

    Jensen, Janni M; Mose, Frank H; Kulik, Anna-Ewa O; Bech, Jesper N; Fenton, Robert A; Pedersen, Erling B

    2015-01-01

    AIM: To quantify changes in urinary excretion of aquaporin2 water channels (u-AQP2), the sodium-potassium-chloride co-transporter (u-NKCC2) and the epithelial sodium channels (u-ENaC) during treatment with bendroflumethiazide (BFTZ), amiloride and placebo. METHODS: In a randomized, double-blinded, placebo-controlled, 3-way crossover study we examined 23 healthy subjects on a standardized diet and fluid intake. The subjects were treated with amiloride 5 mg, BFTZ 1.25 mg or placebo twice a day for 4.5 d before each examination day. On the examination day, glomerular filtration rate was measured by the constant infusion clearance technique with 51Cr-EDTA as reference substance. To estimate the changes in water transport via AQP2 and sodium transport via NKCC2 and ENaC, u-NKCC2, the gamma fraction of ENaC (u-ENaCγ), and u-AQP2 were measured at baseline and after infusion with 3% hypertonic saline. U-NKCC2, u-ENaCγ, u-AQP2 and plasma concentrations of vasopressin (p-AVP), renin (PRC), angiotensin II (p-ANG II) and aldosterone (p-Aldo) were measured, by radioimmunoassay. Central blood pressure was estimated by applanation tonometry and body fluid volumes were estimated by bio-impedance spectroscopy. General linear model with repeated measures or related samples Friedman’s two-way analysis was used to compare differences. Post hoc Bonferroni correction was used for multiple comparisons of post infusion periods to baseline within each treatment group. RESULTS: At baseline there were no differences in u-NKCC2, u-ENaCγ and u-AQP2. PRC, p-Ang II and p-Aldo were increased during active treatments (P < 0.001). After hypertonic saline, u-NKCC2 increased during amiloride (6% ± 34%; P = 0.081) and increased significantly during placebo (17% ± 24%; P = 0.010). U-AQP2 increased significantly during amiloride (31% ± 22%; P < 0.001) and placebo (34% ± 27%; P < 0.001), while u-NKCC2 and u-AQP2 did not change significantly during BFTZ (-7% ± 28%; P = 0.257 and 5% ± 16%; P = 0

  2. Studies of the {boldsymbol{bar{K}}}boldsymbol{N} interaction at DAΦNE

    NASA Astrophysics Data System (ADS)

    Vázquez Doce, O.; Bazzi, M.; Berucci, C.; Bombelli, L.; Bragadireanu, A. M.; Cargnelli, M.; Clozza, A.; Curceanu (Petrascu), C.; d'Uffizi, A.; Fiorini, C.; Ghio, F.; Guaraldo, C.; Iliescu, M.; Ishiwatari, T.; Sandri, P. Levi; Longoni, A.; Marton, J.; Okada, S.; Pietreanu, D.; Piscicchia, K.; Poli Lener, M.; Ponta, T.; Rizzo, A.; Romero Vidal, A.; Sbardella, E.; Scordo, A.; Shi, H.; Sirghi, D. L.; Sirghi, F.; Tatsuno, H.; Widmann, E.; Zmeskal, J.

    AMADEUS is an experiment planned to be performed at the DAΦNE e+e- collider of the Frascati National Laboratories (Italy) of INFN, to investigate the antikaon-nuclei interaction at low energies. AMADEUS will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, including a complete experimental program for the case of the kaonic nuclear clusters. The study of the absorption of antikaon by the nucleus will provide information concerning the bar{K}N interaction and the modification of the kaon mass in the nuclear medium. The experiment is being preceded by the study of the hadronic interactions of K- in the 4He of the drift chamber from the KLOE experiment data.

  3. Studies of the {boldsymbol{bar{K}}}boldsymbol{N} interaction at DAΦNE

    NASA Astrophysics Data System (ADS)

    Vázquez Doce, O.; Bazzi, M.; Berucci, C.; Bombelli, L.; Bragadireanu, A. M.; Cargnelli, M.; Clozza, A.; Curceanu (Petrascu), C.; d'Uffizi, A.; Fiorini, C.; Ghio, F.; Guaraldo, C.; Iliescu, M.; Ishiwatari, T.; Sandri, P. Levi; Longoni, A.; Marton, J.; Okada, S.; Pietreanu, D.; Piscicchia, K.; Poli Lener, M.; Ponta, T.; Rizzo, A.; Romero Vidal, A.; Sbardella, E.; Scordo, A.; Shi, H.; Sirghi, D. L.; Sirghi, F.; Tatsuno, H.; Widmann, E.; Zmeskal, J.

    2012-05-01

    AMADEUS is an experiment planned to be performed at the DAΦNE e+e- collider of the Frascati National Laboratories (Italy) of INFN, to investigate the antikaon-nuclei interaction at low energies. AMADEUS will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, including a complete experimental program for the case of the kaonic nuclear clusters. The study of the absorption of antikaon by the nucleus will provide information concerning the bar{K}N interaction and the modification of the kaon mass in the nuclear medium. The experiment is being preceded by the study of the hadronic interactions of K- in the 4He of the drift chamber from the KLOE experiment data.

  4. A self-injection acceleration test experiment for the FLAME laser

    NASA Astrophysics Data System (ADS)

    Labate, L.; Anelli, F.; Bacci, A.; Batani, D.; Bellaveglia, M.; Benedetti, C.; Benocci, R.; Cacciotti, L.; Cecchetti, C. A.; Ciricosta, O.; Clozza, A.; Cultrera, L.; Di Pirro, G.; Drenska, N.; Faccini, R.; Ferrario, M.; Filippetto, D.; Gallo, S.; Fioravanti, S.; Gamucci, A.; Gatti, G.; Ghigo, A.; Giulietti, A.; Giulietti, D.; Köster, P.; Levato, T.; Lollo, V.; Pace, E.; Pathak, N.; Rossi, A.; Serafini, L.; Turchetti, G.; Vaccarezza, C.; Valente, P.; Vicario, C.; Gizzi, L. A.

    2010-10-01

    A 250-TW laser system (FLAME - Frascati laser for acceleration and multidisciplinary experiments) is now in its commissioning phase in a new laboratory at LNF-INFN in the framework of the PLASMONX (Plasma acceleration and monochromatic X-ray generation) project. The laser will deliver<25 fs duration pulses with an energy up to 6 J, at a 10 Hz repetition rate. An ad hoc target area has also been designed and is currently being set up, allowing the first test experiments of electron laser wakefield acceleration to be carried out over the next few months in a safe, radiation-protected environment. An overview of the main features of the laser system and target area is given, along with a survey of the design and set-up of the self-injection test experiment, which is expected to reach the production of sub-GeV electron bunches.

  5. Unlocking the secrets of the kaon-nucleon/nuclei interactions at low-energies: The SIDDHARTA(-2) and the AMADEUS experiments at the DAΦNE collider

    NASA Astrophysics Data System (ADS)

    Curceanu, C.; Bazzi, M.; Beer, G.; Berucci, C.; Bombelli, L.; Bragadireanu, A. M.; Cargnelli, M.; Clozza, A.; d'Uffizi, A.; Fiorini, C.; Frizzi, T.; Ghio, F.; Guaraldo, C.; Hayano, R. S.; Iliescu, M.; Ishiwatari, T.; Iwasaki, M.; Kienle, P.; Levi Sandri, P.; Longoni, A.; Marton, J.; Okada, S.; Pietreanu, D.; Piscicchia, K.; Poli Lener, M.; Ponta, T.; Romero Vidal, A.; Sbardella, E.; Scordo, A.; Shi, H.; Sirghi, D. L.; Sirghi, F.; Tatsuno, H.; Tudorache, A.; Tudorache, V.; Vazquez Doce, O.; Widmann, E.; Wünschek, B.; Zmeskal, J.

    2013-09-01

    The DAΦNE electron-positron collider at the Laboratori Nazionali di Frascati of INFN has made available a unique quality low-energy negative kaons “beam”, which is being used to unlock the secrets of the kaon-nucleon/nuclei interactions at low energies by the SIDDHARTA(-2) and the AMADEUS experiments. SIDDHARTA has already performed unprecedented precision measurements of kaonic atoms, and is being presently upgraded, as SIDDHARTA-2, to approach new frontiers. The AMADEUS experiment already started a data taking with a dedicated carbon target, plans to perform in the coming years precision measurements on kaon-nuclei interactions at low-energies, in particular to study the possible formation of kaonic nuclei and the Λ(1405). The two experiments are briefly presented in this paper.

  6. Studies of antikaon interactions with nucleons at DAΦNE

    NASA Astrophysics Data System (ADS)

    Doce, O. Vazquez; Bazzi, M.; Berucci, C.; Bosnar, D.; Bragadireanu, M.; Cargnelli, M.; Clozza, A.; Curceanu, C.; Ghio, F.; Guaraldo, C.; Iliescu, M.; Ishiwatari, T.; Kienle, P.; Sandri, P. Levi; Marton, J.; Suzuki, K.; Okada, S.; Piscicchia, K.; Lener, M. Poli; Rizzo, A.; Vidal, A. Romero; Sbardella, E.; Scordo, A.; Sirghi, D. L.; Sirghi, F.; d'Uffizi, A.; Widmann, E.; Zmeskal, J.

    2011-10-01

    AMADEUS is an experiment planned to be performed at the DAFNE e+e- collider of the Frascati National Laboratories (Italy) of INFN, to investigate the antikaon-nuclei interaction at low energies. AMADEUS will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, including a complete experimental program for the case of the kaonic clusters. The study of the absorption of antikaon by the nucleus will provide information concerning the K¯N interaction and the modification of the kaon mass in the nuclear medium. The experiment is being preceded by the study of the hadronic interactions of K- in the 4He of the drift chamber from the KLOE experiment data.

  7. Unveiling the strangeness secrets: low-energy kaon-nucleon/nuclei interactions studies at DAΦNE

    NASA Astrophysics Data System (ADS)

    Curceanu, C.; Bazzi, M.; Beer, G.; Berucci, C.; Bosnar, D.; Bragadireanu, A. M.; Cargnelli, M.; Clozza, A.; D'Uffizi, A.; Fabbietti, L.; Fiorini, C.; Ghio, F.; Guaraldo, C.; Hayano, R. S.; Iliescu, M.; Ishiwatari, T.; Iwasaki, M.; Levi Sandri, P.; Marton, J.; Okada, S.; Pietreanu, D.; Piscicchia, K.; Poli Lener, M.; Ponta, T.; Quaglia, R.; Romero Vidal, A.; Sbardella, E.; Scordo, A.; Shi, H.; Sirghi, D. L.; Sirghi, F.; Tatsuno, H.; Tucaković, I.; Vazquez Doce, O.; Widmann, E.; Zmeskal, J.

    2014-03-01

    The DAΦNE electron-positron collider at the Laboratori Nazionali di Frascati of INFN, Italy has made available a unique quality low-energy negatively charged kaons "beam", which is used to unveil the secrets of the kaon-nucleon/nuclei interactions at low energies by the SIDDHARTA-2 and AMADEUS experiments. SIDDHARTA has already performed unprecedented precision measurements of kaonic atoms, and is being presently upgraded, as SIDDHARTA-2, to approach new frontiers. The AMADEUS experiment plans to perform in the coming years precision measurements on kaon-nuclei interactions at low-energies, to study the possible formation of kaonic nuclei, of the Λ(1405) and of many other processes involving strangeness.

  8. Low-energy kaon-nucleon/nuclei interaction studies at DAΦNE by AMADEUS

    NASA Astrophysics Data System (ADS)

    Tucaković, Ivana; Bazzi, M.; Berucci, C.; Bosnar, D.; Bragadireanu, A. M.; Cargnelli, M.; Clozza, A.; Curceanu, C.; D'Uffizi, A.; Fabbietti, L.; Fiorini, C.; Ghio, F.; Guaraldo, C.; Iliescu, M.; Ishiwatari, T.; Levi Sandri, P.; Marton, J.; Pietreanu, D.; Piscicchia, K.; Poli Lener, M.; Quaglia, R.; Sbardella, E.; Scordo, A.; Shi, H.; Sirghi, D. L.; Sirghi, F.; Tatsuno, H.; Vazquez Doce, O.; Widmann, E.; Zmeskal, J.

    2015-05-01

    The AMADEUS experiment deals with the investigation of the low-energy kaon-nuclei hadronic interaction at the DAΦNE collider at LNF-INFN, fundamental to respond to longstanding open questions in the non-perturbative QCD in the strangeness sector. One of the most interesting aspects is to understand how hadron masses and interactions change in the nuclear environment. The antikaon-nucleon potential is investigated searching for signals from possible bound kaonic clusters, which would imply a strongly attractive antikaon-nucleon potential. AMADEUS step 0 consists in the analysis of 2004/2005 KLOE data, exploring K- absorptions in H, 4He, 9Be and 12C present in setup materials. The status of the various preliminary analyses is presented, together with future perspectives.

  9. PIXE-PIGE Analysis Of Aerosol Composition In Urban Italian Environments

    SciTech Connect

    Nava, S.; D'Alessandro, A.; Prati, P.; Zucchiatti, A.; Lucarelli, F.; Mando, P.A.; Marcazzan, G.; Valli, G.; Vecchi, R.

    2003-08-26

    Fine particulate has become one of the biggest concerns in Italian cities pollution; the study of its composition is a powerful tool to evaluate the effects on health and identify pollution sources. PIXE is an established technique for particulate analysis (being multi-elemental, sensitive, fast, non-destructive and requiring no sample preparations) and has been extensively used, in combination with other IBA techniques, for particulate characterization in Italian urban environments. Here we report the preliminary results on the analysis of the aerosol collected, by two-stage continuous streaker samplers, in two Italian cities, Florence and Milan, during July 2001. Elemental concentrations have been extracted in the fine and coarse fractions, with hourly resolution, by PIXE-PIGE analysis, performed at the 3 MeV external proton beam INFN facility at the University of Florence.

  10. A new H2 (+) source: Conceptual study and experimental test of an upgraded version of the VIS-Versatile ion source.

    PubMed

    Castro, G; Torrisi, G; Celona, L; Mascali, D; Neri, L; Sorbello, G; Leonardi, O; Patti, G; Castorina, G; Gammino, S

    2016-08-01

    The versatile ion source is an off-resonance microwave discharge ion source which produces a slightly overdense plasma at 2.45 GHz of pumping wave frequency extracting more than 60 mA proton beams and 50 mA He(+) beams. DAEδALUS and IsoDAR experiments require high intensities for H2 (+) beams to be accelerated by high power cyclotrons for neutrinos generation. In order to fulfill the new requirements, a new plasma chamber and injection system has been designed and manufactured for increasing the H2 (+) beam intensity. In this paper the studies for the increasing of the H2 (+)/p ratio and for the design of the new plasma chamber and injection system will be shown and discussed together with the experimental tests carried out at Istituto Nazionale di Fisica Nucleare-Laboratori Nazionali del Sud (INFN-LNS) and at Best Cyclotron Systems test-bench in Vancouver, Canada. PMID:27587109

  11. Neutron-rich isotope production using a uranium carbide - carbon nanotubes SPES target prototype

    NASA Astrophysics Data System (ADS)

    Corradetti, S.; Biasetto, L.; Manzolaro, M.; Scarpa, D.; Carturan, S.; Andrighetto, A.; Prete, G.; Vasquez, J.; Zanonato, P.; Colombo, P.; Jost, C. U.; Stracener, D. W.

    2013-05-01

    The SPES (Selective Production of Exotic Species) project, under development at the Istituto Nazionale di Fisica Nucleare - Laboratori Nazionali di Legnaro (INFN-LNL), is a new-generation Isotope Separation On-Line (ISOL) facility for the production of radioactive ion beams by means of the proton-induced fission of uranium. In the framework of the research on the SPES target, seven uranium carbide discs, obtained by reacting uranium oxide with graphite and carbon nanotubes, were irradiated with protons at the Holifield Radioactive Ion Beam Facility (HRIBF) of Oak Ridge National Laboratory (ORNL). In the following, the yields of several fission products obtained during the experiment are presented and discussed. The experimental results are then compared to those obtained using a standard uranium carbide target. The reported data highlights the capability of the new type of SPES target to produce and release isotopes of interest for the nuclear physics community.

  12. Research and development on materials for the SPES target

    NASA Astrophysics Data System (ADS)

    Corradetti, Stefano; Andrighetto, Alberto; Manzolaro, Mattia; Scarpa, Daniele; Vasquez, Jesus; Rossignoli, Massimo; Monetti, Alberto; Calderolla, Michele; Prete, Gianfranco

    2014-03-01

    The SPES project at INFN-LNL (Istituto Nazionale di Fisica Nucleare - Laboratori Nazionali di Legnaro) is focused on the production of radioactive ion beams. The core of the SPES facility is constituted by the target, which will be irradiated with a 40 MeV, 200 µA proton beam in order to produce radioactive species. In order to efficiently produce and release isotopes, the material constituting the target should be able to work under extreme conditions (high vacuum and temperatures up to 2000 °C). Both neutron-rich and proton-rich isotopes will be produced; in the first case, carbon dispersed uranium carbide (UCx) will be used as a target, whereas to produce p-rich isotopes, several types of targets will have to be irradiated. The synthesis and characterization of different types of material will be reported. Moreover, the results of irradiation and isotopes release tests on different uranium carbide target prototypes will be discussed.

  13. Ionization efficiency estimations for the SPES surface ion source

    NASA Astrophysics Data System (ADS)

    Manzolaro, M.; Andrighetto, A.; Meneghetti, G.; Rossignoli, M.; Corradetti, S.; Biasetto, L.; Scarpa, D.; Monetti, A.; Carturan, S.; Maggioni, G.

    2013-12-01

    Ion sources play a crucial role in ISOL (Isotope Separation On Line) facilities determining, with the target production system, the ion beam types available for experiments. In the framework of the SPES (Selective Production of Exotic Species) INFN (Istituto Nazionale di Fisica Nucleare) project, a preliminary study of the alkali metal isotopes ionization process was performed, by means of a surface ion source prototype. In particular, taking into consideration the specific SPES in-target isotope production, Cs and Rb ion beams were produced, using a dedicated test bench at LNL (Laboratori Nazionali di Legnaro). In this work the ionization efficiency test results for the SPES Ta surface ion source prototype are presented and discussed.

  14. Development of a scintillation-fiber detector for real-time particle tracking

    NASA Astrophysics Data System (ADS)

    Lo Presti, D.; Bonanno, D. L.; Longhitano, F.; Pugliatti, C.; Russo, G. V.; Aiello, S.; Cirrone, G. A. P.; Giordano, V.; Leonora, E.; Randazzo, N.; Romano, F.; Russo, M.; Sipala, V.; Stancampiano, C.; Reito, S.

    2013-04-01

    The prototype of the OFFSET (Optical Fiber Folded Scintillating Extended Tracker) tracker is presented. It exploits a novel system for particle tracking, designed to achieve real-time imaging, large detection areas, and a high spatial resolution especially suitable for use in medical diagnostics. The main results regarding the system architecture have been used as a demonstration of the technique which has been patented by the Istituto Nazionale di Fisica Nucleare (INFN). The prototype of this tracker, presented in this paper, has a 20 × 20 cm2 sensitive area, consisting of two crossed ribbons of 500 micron square scintillating fibers. The track position information is extracted in real time in an innovative way, using a reduced number of read-out channels to obtain very large detection area with moderate enough costs and complexity. The performance of the tracker was investigated using beta sources, cosmic rays, and a 62 MeV proton beam.

  15. Noise reduction in muon tomography for detecting high density objects

    NASA Astrophysics Data System (ADS)

    Benettoni, M.; Bettella, G.; Bonomi, G.; Calvagno, G.; Calvini, P.; Checchia, P.; Cortelazzo, G.; Cossutta, L.; Donzella, A.; Furlan, M.; Gonella, F.; Pegoraro, M.; Rigoni Garola, A.; Ronchese, P.; Squarcia, S.; Subieta, M.; Vanini, S.; Viesti, G.; Zanuttigh, P.; Zenoni, A.; Zumerle, G.

    2013-12-01

    The muon tomography technique, based on multiple Coulomb scattering of cosmic ray muons, has been proposed as a tool to detect the presence of high density objects inside closed volumes. In this paper a new and innovative method is presented to handle the density fluctuations (noise) of reconstructed images, a well known problem of this technique. The effectiveness of our method is evaluated using experimental data obtained with a muon tomography prototype located at the Legnaro National Laboratories (LNL) of the Istituto Nazionale di Fisica Nucleare (INFN). The results reported in this paper, obtained with real cosmic ray data, show that with appropriate image filtering and muon momentum classification, the muon tomography technique can detect high density materials, such as lead, albeit surrounded by light or medium density material, in short times. A comparison with algorithms published in literature is also presented.

  16. Recent developments on techniques for differential phase imaging at the medical beamline of ELETTRA

    NASA Astrophysics Data System (ADS)

    Arfelli, F.; Pelliccia, D.; Cedola, A.; Astolfo, A.; Bukreeva, I.; Cardarelli, P.; Dreossi, D.; Lagomarsino, S.; Longo, R.; Rigon, L.; Sodini, N.; Menk, R. H.

    2013-06-01

    Over the last decade different phase contrast approaches have been exploited at the medical beamline SYRMEP of the synchrotron radiation facility Elettra in Trieste, Italy. In particular special focus has been drawn to analyzer based imaging and the associated imaging theory and processing. Analyzer based Imaging (ABI) and Diffraction Enhanced Imaging (DEI) techniques have been successfully applied in several biomedical applications. Recently it has been suggested to translate the acquired knowledge in this field towards a Thomson Backscattering Source (TBS), which is presently under development at the Frascati National Laboratories of INFN (Istituto Nazionale di Fisica Nucleare) in Rome, Italy. Such source is capable of producing intense and quasi-monochromatic hard X-ray beams. For the technical implementation of biomedical phase imaging at the TBS a grating interferometer for differential phase contrast imaging has been designed and successfully tested at SYRMEP beamline.

  17. A steady-state high-temperature method for measuring thermal conductivity of refractory materials

    NASA Astrophysics Data System (ADS)

    Manzolaro, M.; Corradetti, S.; Andrighetto, A.; Ferrari, L.

    2013-05-01

    A new methodology and an instrumental setup for the thermal conductivity estimation of isotropic bulk graphite and different carbides at high temperatures are presented. The method proposed in this work is based on the direct measurement of temperature and emissivity on the top surface of a sample disc of known dimensions. Temperatures measured under steady-state thermal equilibrium are then used to estimate the thermal conductivity of the sample by making use of the inverse parameter estimation technique. Thermal conductivity values obtained in this way are then compared to the material data sheets and values found in literature. The reported work has been developed within the Research and Development framework of the SPES (Selective Production of Exotic Species) project at INFN-LNL (Istituto Nazionale di Fisica Nucleare - Laboratori Nazionali di Legnaro).

  18. PREFACE: Young Researcher Meeting, Trieste 2013

    NASA Astrophysics Data System (ADS)

    Agostini, F.; Antolini, C.; Aversa, R.; Cattani, G.; Martinelli, M.; Mazzaferro, L.; Migliaccio, M.; Paci, F.; Pietrobon, D.; Ricci Pacifici, D.; Stellato, F.; Veneziani, M.

    2013-12-01

    YRM logo The Young Researcher Meeting (www.yrmr.it) has been a rapidly growing event for the last few years. Together with other initiatives which have emerged in several research areas, the young researcher meeting marks the awareness and the desire of PhD students, postdoctoral fellows and young researchers to play a major role in scientific progress. Devoted to the discussion and the interchange of new developments and ideas in physics, the meeting is primarily aimed at graduate students and postdoctoral researchers, who are encouraged to present their work in an informal atmosphere. One of the main purposes of the conference is to create an international network of young researchers, both experimentalists and theorists, and fruitful collaborations across the different branches of physics. Born in Rome in 2009, after three editions that strengthened it, the Young Researcher Meeting 2013 was held in Trieste. Propelled by the past success, the fourth meeting was a two-day conference on 3-4June. It was sponsored by the International School for Advanced Studies - SISSA - and the University of Padova, thus acquiring an even further international drive. In this volume, we collect some of the contributions that were presented at the conference. They cover topics in astrophysics and cosmology, particle and theoretical physics, soft and condensed matter, biophysics and medical physics. YRM Organising and Editorial Committee Fabio Agostini (fabio.agostini@roma2.infn.it) Dipartimento di Fisica, Università di Roma 'Tor Vergata' Via della Ricerca Scientifica 1, 00133 Roma, Italy Claudia Antolini (claudia.antolini@sissa.it) SISSA - Scuola Internazionale Superiore di Studi Avanzati Via Bonomea 265, 34136 Trieste, Italy Rossella Aversa (raversa@sissa.it) SISSA - Scuola Internazionale Superiore di Studi Avanzati Via Bonomea 265, 34136 Trieste, Italy Giordano Cattani (giordano.cattani@gmail.com) Matteo Martinelli (mmartin@sissa.it) SISSA - Scuola Internazionale Superiore di

  19. Hypernuclear Spectroscopy via (e,e'K+) in JLab's Hall A

    SciTech Connect

    John J. LeRose; A. Acha; P. Bydzovsky; C. Chang; C.C. Chang; E. Cisbani; F. Cusano; C.W. de Jager; R. De Leo; R.J. Feuerbach; S. Frullani; F. Garibaldi; D.W. Higinbotham; M. Iodice; L. Lagamba; P. Markowitz; S. Marrone; B. Reitz; M. Sotona; Miloslav Sotona; G.M. Urciuoli

    2007-12-17

    Results are presented from a new experiment (E94-107) in Hall A of the Thomas Jefferson National Accelerator Facility (JLab) producing {12/Lambda} B, {16/Lambda}N, and {9/Lambda}Li using electroproduction, (e,e'K+). In the hypernuclear missing-mass spectrum the experiment achieves very good energy resolution (670 keV FWHM) by exploiting the characteristics of the High Resolution spectrometer pair and the exceptional beam quality available at JLab. The spectrometers were used with the addition an INFN provided pair of septum magnets to reach the desired small angles. Also, the Hall A standard complement of equipment was further augmented by the addition of a Ring Imaging Cherenkov detector (RICH) to achieve the best possible kaon identification.

  20. Hypernuclear Spectroscopy in JLab's Hall A

    SciTech Connect

    John J. LeRose; A. Acha; P. Bydzovsky; C.C. Chang; E. Cisbani; F. Cusano; C.W. de Jager; R. De Leo; R.J. Feuerbach; S. Frullani; F. Garibaldi; D.W. Higinbotham; M. Iodice; L. Lagamba; P. Markowitz; S. Marrone; B. Reitz; M. Sotona; Miloslav Sotona; G.M. Urciuoli

    2006-10-10

    Results are presented from a new experiment (E94-107) in Hall A of the Thomas Jefferson National Accelerator Facility (JLab) producing Boron-12-lambda using electroproduction, (e,e{prime}K+). In the hypernuclear missing-mass spectrum the experiment achieves very good energy resolution (640 keV FWHM) by exploiting the characteristics of the High Resolution spectrometer pair and the exceptional beam quality available at JLab. The spectrometers were used with the addition an INFN provided pair of septum magnets to reach the desired small angles. Also, the Hall A standard complement of equipment was further augmented by the addition of a Ring Imaging Cherenkov detector (RICH) to achieve the best possible kaon identification.

  1. Background modeling for the GERDA experiment

    NASA Astrophysics Data System (ADS)

    Becerici-Schmidt, N.; Gerda Collaboration

    2013-08-01

    The neutrinoless double beta (0νββ) decay experiment GERDA at the LNGS of INFN has started physics data taking in November 2011. This paper presents an analysis aimed at understanding and modeling the observed background energy spectrum, which plays an essential role in searches for a rare signal like 0νββ decay. A very promising preliminary model has been obtained, with the systematic uncertainties still under study. Important information can be deduced from the model such as the expected background and its decomposition in the signal region. According to the model the main background contributions around Qββ come from 214Bi, 228Th, 42K, 60Co and α emitting isotopes in the 226Ra decay chain, with a fraction depending on the assumed source positions.

  2. GERDA - a new neutrinoless double beta experiment using 76Ge

    NASA Astrophysics Data System (ADS)

    Meierhofer, G.; GERDA Collaboration

    2011-09-01

    The search for neutrinoless double beta decay (0νßß) has been a very active field for the last decades. While double beta decay has been observed, 0νßß decay still waits for its experimental proof. The GErmanium Detector Array (GERDA) uses 76Ge, an ideal candidate as it is acting as source and detector simultaneously. Germanium detectors, isotopically enriched in 76Ge are submerged directly into an ultra pure cryo liquid, which serves as coolant and radiation shield. This concept will allow to reduce the background by up to two orders of magnitude with respect to earlier experiments. GERDA has been constructed in hall A of the underground laboratory LNGS of the INFN in Italy. The experiment started recently with a test run.

  3. Measurement of the half-life of the two-neutrino double beta decay of 76Ge with the GERDA experiment

    NASA Astrophysics Data System (ADS)

    The GERDA Collaboration; Agostini, M.; Allardt, M.; Andreotti, E.; Bakalyarov, A. M.; Balata, M.; Barabanov, I.; Barnabé Heider, M.; Barros, N.; Baudis, L.; Bauer, C.; Becerici-Schmidt, N.; Bellotti, E.; Belogurov, S.; Belyaev, S. T.; Benato, G.; Bettini, A.; Bezrukov, L.; Bode, T.; Brudanin, V.; Brugnera, R.; Budjáš, D.; Caldwell, A.; Cattadori, C.; Chernogorov, A.; Cossavella, F.; Demidova, E. V.; Denisov, A.; Domula, A.; Egorov, V.; Falkenstein, R.; Ferella, A. D.; Freund, K.; Froborg, F.; Frodyma, N.; Gangapshev, A.; Garfagnini, A.; Gazzana, S.; Grabmayr, P.; Gurentsov, V.; Gusev, K.; Guthikonda, K. K.; Hampel, W.; Hegai, A.; Heisel, M.; Hemmer, S.; Heusser, G.; Hofmann, W.; Hult, M.; Inzhechik, L. V.; Ioannucci, L.; Janicskó Csáthy, J.; Jochum, J.; Junker, M.; Kianovsky, S.; Kirpichnikov, I. V.; Kirsch, A.; Klimenko, A.; Knöpfle, K. T.; Kochetov, O.; Kornoukhov, V. N.; Kuzminov, V.; Laubenstein, M.; Lazzaro, A.; Lebedev, V. I.; Lehnert, B.; Liao, H. Y.; Lindner, M.; Lippi, I.; Liu, X.; Lubashevskiy, A.; Lubsandorzhiev, B.; Lutter, G.; Machado, A. A.; Majorovits, B.; Maneschg, W.; Nemchenok, I.; Nisi, S.; O'Shaughnessy, C.; Pandola, L.; Pelczar, K.; Peraro, L.; Pullia, A.; Riboldi, S.; Ritter, F.; Sada, C.; Salathe, M.; Schmitt, C.; Schönert, S.; Schreiner, J.; Schulz, O.; Schwingenheuer, B.; Shevchik, E.; Shirchenko, M.; Simgen, H.; Smolnikov, A.; Stanco, L.; Strecker, H.; Tarka, M.; Ur, C. A.; Vasenko, A. A.; Volynets, O.; von Sturm, K.; Walter, M.; Wegmann, A.; Wojcik, M.; Yanovich, E.; Zavarise, P.; Zhitnikov, I.; Zhukov, S. V.; Zinatulina, D.; Zuber, K.; Zuzel, G.

    2013-03-01

    The primary goal of the GERmanium Detector Array (GERDA) experiment at the Laboratori Nazionali del Gran Sasso of INFN is the search for the neutrinoless double beta decay of 76Ge. High-purity germanium detectors made from material enriched in 76Ge are operated directly immersed in liquid argon, allowing for a substantial reduction of the background with respect to predecessor experiments. The first 5.04 kg yr of data collected in Phase I of the experiment have been analyzed to measure the half-life of the neutrino-accompanied double beta decay of 76Ge. The observed spectrum in the energy range between 600 and 1800 keV is dominated by the double beta decay of 76Ge. The half-life extracted from GERDA data is T2ν1/2 = (1.84+0.14-0.10) × 1021 yr.

  4. First results of neutrinoless double beta decay search with the GERmanium Detector Array "GERDA"

    NASA Astrophysics Data System (ADS)

    Janicskó Csáthy, József

    2014-06-01

    The study of neutrinoless double beta decay is the most powerful approach to the fundamental question if the neutrino is a Majorana particle, i.e. its own anti-particle. The observation of the lepton number violating neutrinoless double beta decay would establish the Majorana nature of the neutrino. Until now neutrinoless double beta decay was not observed. The GERmanium Detector Array, GERDA is a double beta decay experiment located at the INFN Gran Sasso National Laboratory, Italy. GERDA operates bare Ge diodes enriched in 76Ge in liquid argon supplemented by a water shield. The exposure accumulated adds up to 21.6 kg· yr with a background level of 1.8 · 10-2 cts/(keV·kg·yr). The results of the Phase I of the experiment are presented and the preparation of the Phase II is briefly discussed.

  5. Heat Deposit Calculation in Spallation Unit

    NASA Astrophysics Data System (ADS)

    Karmanov, F. I.; Travleev, A. A.; Latysheva, L. N.; Vecchi, M.

    2001-11-01

    The present study concerns the calculation of the heat deposition in one of the EAP-80 basic units - the spallation module including the beam window, lead-bismuth spallation target and primary liquid metal cooling system. It is assumed that the model of sub-critical reactor under investigation is based on ANSALDO-INFN-ENEA-CRS4 reference configuration1. The calculation have been done by means of a couple computer codes: INCC 2 and GEANT3.21 3. These codes have been preliminary tested on the experimental data obtained in 4 for the case of interaction of proton beam and lead-bismuth targets at the energy Ep= 800MeV which is close to energy range relevant for ADS configuration.

  6. Reaction dynamics induced by the radioactive ion beam {sup 7}Be on medium-mass and heavy targets

    SciTech Connect

    Mazzocco, M. Stefanini, C.; Strano, E.; Torresi, D.; Lay, J. A.; Molini, P.; Soramel, F.; Boiano, A.; Parascandolo, C.; Pierroutsakou, D.; Di Meo, P.; Boiano, C.; La Commara, M.; Sandoli, M.; Silvestri, R.; Manea, C.; Nicoletto, M.; Acosta, L.; Fernandez-Garcia, J. P.; Glodariu, T.; and others

    2015-10-15

    We studied the reaction dynamics induced at Coulomb barrier energies by the weakly-bound Radioactive Ion Beam {sup 7}Be (S{sub α} = 1.586 MeV) on medium-mass ({sup 58}Ni) and heavy ({sup 208}Pb) targets. The experiments were performed at INFN-LNL (Italy), where a 2-3×10{sup 5} pps {sup 7}Be secondary beam was produced with the RIB in-flight facility EXOTIC. Charged reaction products were detected by means of high-granularity silicon detectors in rather wide angular ranges. The contribution presents an up-to-date status of the data analysis and theoretical interpretation for both systems.

  7. Microwave field distribution and electron cyclotron resonance heating process

    SciTech Connect

    Consoli, F.; Celona, L.; Ciavola, G.; Gammino, S.; Maimone, F.; Barbarino, S.; Catalano, R. S.; Mascali, D.

    2008-02-15

    In an electron cyclotron resonance ion source, ions are produced from a plasma generated and sustained by microwaves with a proper frequency. Some experiments showed that the plasma formation, the consequent amount of particles extracted from the source, and the related beam shape strongly depend on the frequency of the electromagnetic wave feeding the cavity. In order to have a better understanding of these phenomena, in this work we deal with the description of the motion of a charged particle inside the plasma chamber model of the SERSE ion source operating at INFN-LNS in Catania, the analysis being applicable to any similar apparatus. The electromagnetic fields inside the vacuum filled chamber were determined theoretically and, together with proper simulations, their fundamental role on the particle motion, on their confinement, and on the energy transfer they are subjected to during their motion within the cavity is shown.

  8. First results in the application of silicon photomultiplier matrices to small animal PET

    NASA Astrophysics Data System (ADS)

    Llosá, G.; Belcari, N.; Bisogni, M. G.; Collazuol, G.; Marcatili, S.; Boscardin, M.; Melchiorri, M.; Tarolli, A.; Piemonte, C.; Zorzi, N.; Barrillon, P.; Bondil-Blin, S.; Chaumat, V.; de La Taille, C.; Dinu, N.; Puill, V.; Vagnucci, J.-F.; Del Guerra, A.

    2009-10-01

    A very high resolution small animal PET scanner that employs matrices of silicon photomultipliers as photodetectors is under development at the University of Pisa and INFN Pisa. The first SiPM matrices composed of 16 (4×4)1 mm×1 mm pixel elements on a common substrate have been produced at FBK-irst, and are being evaluated for this application. The MAROC2 ASIC developed at LAL-Orsay has been employed for the readout of the SiPM matrices. The devices have been tested with pixelated and continuous LYSO crystals. The results show the good performance of the matrices and lead to the fabrication of matrices with 64 SiPM elements.

  9. Pulse-shape discrimination in NE213 liquid scintillator detectors

    NASA Astrophysics Data System (ADS)

    Cavallaro, M.; Tropea, S.; Agodi, C.; Assié, M.; Azaiez, F.; Boiano, C.; Bondì, M.; Cappuzzello, F.; Carbone, D.; De Napoli, M.; de Séréville, N.; Foti, A.; Linares, R.; Nicolosi, D.; Scarpaci, J. A.

    2013-02-01

    The 16-channel fast stretcher BaFPro module, originally developed for processing signals of Barium Fluoride scintillators, has been modified to make a high performing analog pulse-shape analysis of signals from the NE213 liquid scintillators of the EDEN neutron detector array. The module produces two Gaussian signals, whose amplitudes are proportional to the height of the fast component of the output light and to the total energy deposited into the scintillator, respectively. An in-beam test has been performed at INFN-LNS (Italy) demonstrating a low detection threshold, a good pulse-shape discrimination even at low energies and a wide dynamic range for the measurement of the neutrons energy.

  10. PIXE microbeam analysis of the metallic debris release around endosseous implants

    NASA Astrophysics Data System (ADS)

    Buso, G. P.; Galassini, S.; Moschini, G.; Passi, P.; Zadro, A.; Uzunov, N. M.; Doyle, B. L.; Rossi, P.; Provencio, P.

    2005-10-01

    The mechanical friction that occurs during the surgical insertion of endosseous implants, both in dentistry and orthopaedics, may cause the detachment of metal debris which are dislodged into the peri-implant tissues and can lead to adverse clinical effects. This phenomenon more likely happens with coated or roughened implants that are the most widely employed. In the present study were studied dental implants screws made of commercially pure titanium and coated using titanium plasma-spray (TPS) technique. The implants were inserted in the tibia of rabbits, and removed "en bloc" with the surrounding bone after one month. After proper processing and mounting on plastic holders, samples from bones were analysed by EDXRF setup at of National Laboratories of Legnaro, INFN, Italy, and consequently at 3 MeV proton microbeam setup at Sandia National Laboratories. Elemental maps were drawn, showing some occasional presence of metal particles in the peri-implant bone.

  11. SPECIAL SEMINAR - The NOTTE experiment, or how to become a Total Solar Eclipse chaser

    ScienceCinema

    None

    2011-10-06

    The NOTTE experiment (Neutrino Oscillations with Telescope during Total Eclipse) aims at searching for visible photons emitted through a possible radiative decay of solar neutrinos. The experiment and the expeditions organized by a group of physicists and astrophysicists from INFN and INAF Bologna hunting for Total Solar Eclipses from 1998 to 2006 wil be described. The results of observations performed during total solar eclipse expeditions in 2001 (Zambia) and 2006 (Sahara desert, Libya) are presented and a beautiful photo gallery will be shown. Other peculiar observations that can be made during a solar eclipse are also illustrated. The seminar will be followed by a brief presentation of future camps for solar eclipse chasers and scientists organized in 2008 in Russia, Kazakhstan, China and Mongolia, in 2009 in Shanghai and on the Easter Island in 2010.

  12. Updated Design of the Italian SuperB Factory Injection System

    SciTech Connect

    Guiducci, S.; Biagini, M.E.; Boni, R.; Preger, M.; Raimondi, P.; Chance, A.; Brossard, J.; Dadoun, O.; Lepercq, P.; Rimbault, C.; Variola, A.; Seeman, J.; /SLAC

    2012-04-26

    The ultra high luminosity B-factory (SuperB) project of INFN requires a high performance and reliable injection system, providing electrons at 4 GeV and positrons at 7 GeV, to fulfill the very tight requirements of the collider. Due to the short beam lifetime, continuous injection of electrons and positrons in both High Energy Ring (HER) and Low Energy Ring (LER) is necessary to keep the average luminosity at a high level. An updated version of the injection system, optimized at higher repetition frequency is presented. This scheme includes a polarized electron gun, a positron production scheme with electron/positron conversion at low energy 0.6 GeV, and a 1 GeV damping ring to reduce the injected emittance of the positron beam.

  13. The 12C* Hoyle state in the inelastic 12C + 12C reaction and in 24Mg* decay

    NASA Astrophysics Data System (ADS)

    Morelli, L.; Bruno, M.; D'Agostino, M.; Baiocco, G.; Gulminelli, F.; Abbondanno, U.; Barlini, S.; Bini, M.; Casini, G.; Cinausero, M.; Degerlier, M.; Fabris, D.; Gramegna, F.; Mabiala, J.; Marchi, T.; Olmi, A.; Pasquali, G.; Piantelli, S.; Valdré, S.

    2016-08-01

    The reaction 12C + 12C at 95 MeV has been studied at the Legnaro Laboratories of INFN with the GARFIELD + RCo apparatus. Data have been analyzed in order to investigate the decay of the Hoyle state of 12C*. Two different data selections have been made. The first one corresponds to peripheral binary collisions where the quasi-projectile is excited to the Hoyle state and the target has been left in the ground state. The second selection allows for studying central events with the formation of a 24Mg* and the Hoyle state is obtained as a step of the decay chain. The characteristics of the Hoyle state decay are very similar in the two samples and point to a mainly sequential decay through the population of an intermediate 8Begs, with a small contribution (˜1.1%) from simultaneous three α-particle processes.

  14. Study of cluster structures in 10Be and 16C neutron-rich nuclei via break-up reactions

    NASA Astrophysics Data System (ADS)

    Dell'Aquila, D.; Acosta, L.; Amorini, F.; Andolina, R.; Auditore, L.; Berceanu, I.; Cardella, G.; Chatterjiee, M. B.; De Filippo, E.; Francalanza, L.; Gnoffo, B.; Grzeszczuk, A.; Lanzalone, G.; Lombardo, I.; Martorana, N.; Minniti, T.; Pagano, A.; Pagano, E. V.; Papa, M.; Pirrone, S.; Politi, G.; Pop, A.; Porto, F.; Quattrocchi, L.; Rizzo, F.; Rosato, E.; Russotto, P.; Trifirò, A.; Trimarchi, M.; Verde, G.; Vigilante, M.

    2016-05-01

    Projectile break-up reactions induced on polyethylene (CH2) target are used in order to study the spectroscopy of 10Be and 16C nuclei. For the present experiment we used 10Be and 16C beams delivered by the FRIBs facility at INFN-LNS, and the CHIMERA 4π multi-detector. 10Be and 16C structures are studied via a relative energy analysis of break-up fragments. The 4He+6He break-up channel allowed us to study the spectroscopy of 10Be; in particular we find evidence of a new state in 10Be at 13.5 MeV excitation energy. The 16C nucleus is studied via 6He-10Be correlation; we find the fingerprint of a possible state at about 20.6 MeV

  15. Study of two- and multi-particle correlations in 12C+24Mg and 12C+208Pb reactions at E=35 AMeV

    NASA Astrophysics Data System (ADS)

    Quattrocchi, L.; Acosta, L.; Amorini, F.; Anzalone, A.; Auditore, L.; Berceanu, I.; Cardella, G.; Chbihi, A.; De Filippo, E.; Dell'Aquila, D.; Francalanza, L.; Gnoffo, B.; Grzeszczuk, A.; Lanzalone, G.; Lombardo, I.; Martel, I.; Minniti, T.; Norella, S.; Pagano, A.; Pagano, E. V.; Papa, M.; Pirrone, S.; Politi, G.; Porto, F.; Rizzo, F.; Rosato, E.; Russotto, P.; Trifirò, A.; Trimarchi, M.; Verde, G.; Veselsky, M.; Vigilante, M.

    2016-05-01

    Two and multi particle correlations from the decay of sources produced in 12C+24Mg and 12C+208Pb collisions at E=35 AMeV have been studied by using the forward part (1° < θlab < 30°) of the CHIMERA multi-detector. Correlations and invariant mass spectroscopy are used to explore simultaneous and sequential decays of resonances in light isotopes with Z˜3-6, produced in peripheral collisions via the break-up of excited quasi-projectiles. Among them we mention 5Li, 6Li, 6Be, 8Be and the astrophysically important state in 12C decaying into three alpha particles. Results and future perspectives at the INFN-LNS will be presented.

  16. RELAP5-3D thermal hydraulic analysis of the target cooling system in the SPES experimental facility

    NASA Astrophysics Data System (ADS)

    Giardina, M.; Castiglia, F.; Buffa, P.; Palermo, G.; Prete, G.

    2014-11-01

    The SPES (Selective Production of Exotic Species) experimental facility, under construction at the Italian National Institute of Nuclear Physics (INFN) Laboratories of Legnaro, Italy, is a second generation Isotope Separation On Line (ISOL) plant for advanced nuclear physic studies. The UCx target-ion source system works at temperature of about 2273 K, producing a high level of radiation (105 Sv/h), for this reason a careful risk analysis for the target chamber is among the major safety issues. In this paper, the obtained results of thermofluid-dynamics simulations of accidental transients in the SPES target cooling system are reported. The analysis, performed by using the RELAP5-3D 2.4.2 qualified thermal-hydraulic system code, proves good safety performance of this system during different accidental conditions.

  17. Studies of antikaon interactions with nucleons at DA{Phi}NE

    SciTech Connect

    Vazquez Doce, O.; Bazzi, M.; Berucci, C.; Clozza, A.; Curceanu, C.; Guaraldo, C.; Iliescu, M.; Sandri, P. Levi; Okada, S.; Piscicchia, K.; Lener, M. Poli; Rizzo, A.; Vidal, A. Romero; Sbardella, E.; Scordo, A.; Sirghi, D. L.; Sirghi, F.; D'Uffizi, A.; Bosnar, D.; Bragadireanu, M.

    2011-10-21

    AMADEUS is an experiment planned to be performed at the DAFNE e{sup +}e{sup -} collider of the Frascati National Laboratories (Italy) of INFN, to investigate the antikaon-nuclei interaction at low energies. AMADEUS will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, including a complete experimental program for the case of the kaonic clusters. The study of the absorption of antikaon by the nucleus will provide information concerning the K-barN interaction and the modification of the kaon mass in the nuclear medium.The experiment is being preceded by the study of the hadronic interactions of K{sup -} in the {sup 4}He of the drift chamber from the KLOE experiment data.

  18. Gallex status report as of November, 1991

    NASA Astrophysics Data System (ADS)

    Kirsten, Till; Anselmann, P.; Hampel, W.; Heusser, G.; Kiko, J.; Kirsten, T.; Monninger, G.; Pernicka, E.; Plaga, R.; Povh, B.; Roenn, U.; Sann, M.; Schlosser, C.; Völk, H.; Wink, R.; Wójcik, M.; Ammon, R. V.; Ebert, K.; Fritsch, T.; Hellriegel, K.; Henrich, E.; Stieglitz, L.; Balata, M.; Bellotti, E.; Cattadori, C.; Ferraris, N.; Lalla, H.; Pezzoni, S.; Stolarczyk, T.; Cremonesi, O.; Fiorini, E.; Ragazzi, S.; Zanotti, L.; v. Feilitzsch, F.; Mößbauer, R.; Schanda, U.; Berthomieu, G.; Schatzman, E.; D'Angelo, S.; Bacci, C.; Belli, P.; Bernabei, R.; Paoluzi, L.; Carmi, I.; Dostrovsky, I.; Charbit, S.; Cribier, M.; Dupont, G.; Gosset, L.; Rich, J.; Spiro, M.; Tao, C.; Vignaud, D.; Hahn, R. L.; Hartmann, F. X.; Rowley, J. K.; Stoenner, R. W.; Weneser, J.

    1992-07-01

    Since 18 months, the radiochemical Gallium-Solar-Neutrino Experiment "GALLEX" is fully operative at the INFN-Gran Sasso Underground Laboratory (LNGS) in Central Italy. Among other things, 41 full scale experiments have been performed since June 1990, establishing the technical aspects of the experiment to be successful in every respect, as designed. The first 28 runs were needed to reduce the amount of disturbing cosmogenic 68Ge activity imported from overground to that level at which any remaining activity is no longer distinguishable from the extremely low counter background level for which the experiment is designed. This milestone was achieved, after a long period of heating the target, in May 1991. Since then, GALLEX monitors solar neutrinos undisturbed, runs B29-B41, so far. At least 10 months of data taking are required to achieve even a prelininary result. For a statistically more meaningful result, the experimental conception calls for 4 years of data taking.

  19. Discovering forgeries of modern art by the 14C Bomb Peak

    NASA Astrophysics Data System (ADS)

    Caforio, L.; Fedi, M. E.; Mandò, P. A.; Minarelli, F.; Peccenini, E.; Pellicori, V.; Petrucci, F. C.; Schwartzbaum, P.; Taccetti, F.

    2014-01-01

    Exploiting the anomalous behaviour of the atmospheric radiocarbon concentration in the years after 1955 (so-called "Bomb Peak"), an alleged painting by Fernard Léger was unambiguously proven to be a fake just by dating the canvas support. Some art historians had questioned the authenticity of the painting, and their suspicions were fuelled by some scientific examinations of the paint materials (X-ray radiography, SEM-EDS), compared to those of another work unquestionably attributed to Léger. The decisive argument to prove that it was a fake was provided by the radiocarbon date obtained from a sample of canvas of the painting, using Accelerator Mass Spectrometry at the INFN-Labec laboratory in Florence. Beyond any doubt, the cotton plant from which the canvas was produced was cut no earlier than 1959, i.e. four years after Léger's death, thus definitely confirming the concerns of a fake.

  20. VizieR Online Data Catalog: 454 VCC galaxies revised coordinates (di Serego Alighieri+, 2013)

    NASA Astrophysics Data System (ADS)

    di Serego Alighieri, S.; Bianchi, S.; Pappalardo, C.; Zibetti, S.; Auld, R.; Baes, M.; Bendo, G.; Corbelli, E.; Davies, J. I.; Davis, T.; de Looze, I.; Fritz, J.; Gavazzi, G.; Giovanardi, C.; Grossi, M.; Hunt, L. K.; Magrini, L.; Pierini, D.; Xilouris, E. M.

    2013-08-01

    The sample of ETG to be searched for dust within HeViCS was selected at optical wavelengths using the GOLDMine compilation (Gavazzi et al. 2003A&A...400..451G, http:goldmine.mib.infn.it), which is mostly based on the Virgo Cluster Catalogue (VCC, Binggeli et al. 1985, Cat. J/AJ/90/1681, 1993A&AS...98..275B), including all morphological types from -3 to 2 (i.e. galaxies earlier than S0a-S0/Sa) and excluding galaxies with radial velocity larger than 3000km/s, since these are background galaxies; we have retained galaxies without a measured radial velocity. (1 data file).

  1. Femtosecond timing-jitter between photo-cathode laser and ultra-short electron bunches by means of hybrid compression

    NASA Astrophysics Data System (ADS)

    Pompili, R.; Anania, M. P.; Bellaveglia, M.; Biagioni, A.; Castorina, G.; Chiadroni, E.; Cianchi, A.; Croia, M.; Di Giovenale, D.; Ferrario, M.; Filippi, F.; Gallo, A.; Gatti, G.; Giorgianni, F.; Giribono, A.; Li, W.; Lupi, S.; Mostacci, A.; Petrarca, M.; Piersanti, L.; Di Pirro, G.; Romeo, S.; Scifo, J.; Shpakov, V.; Vaccarezza, C.; Villa, F.

    2016-08-01

    The generation of ultra-short electron bunches with ultra-low timing-jitter relative to the photo-cathode (PC) laser has been experimentally proved for the first time at the SPARC_LAB test-facility (INFN-LNF, Frascati) exploiting a two-stage hybrid compression scheme. The first stage employs RF-based compression (velocity-bunching), which shortens the bunch and imprints an energy chirp on it. The second stage is performed in a non-isochronous dogleg line, where the compression is completed resulting in a final bunch duration below 90 fs (rms). At the same time, the beam arrival timing-jitter with respect to the PC laser has been measured to be lower than 20 fs (rms). The reported results have been validated with numerical simulations.

  2. Microwave frequency sweep interferometer for plasma density measurements in ECR ion sources: Design and preliminary results

    NASA Astrophysics Data System (ADS)

    Torrisi, Giuseppe; Mascali, David; Neri, Lorenzo; Leonardi, Ornella; Sorbello, Gino; Celona, Luigi; Castro, Giuseppe; Agnello, Riccardo; Caruso, Antonio; Passarello, Santi; Longhitano, Alberto; Isernia, Tommaso; Gammino, Santo

    2016-02-01

    The Electron Cyclotron Resonance Ion Sources (ECRISs) development is strictly related to the availability of new diagnostic tools, as the existing ones are not adequate to such compact machines and to their plasma characteristics. Microwave interferometry is a non-invasive method for plasma diagnostics and represents the best candidate for plasma density measurement in hostile environment. Interferometry in ECRISs is a challenging task mainly due to their compact size. The typical density of ECR plasmas is in the range 1011-1013 cm-3 and it needs a probing beam wavelength of the order of few centimetres, comparable to the chamber radius. The paper describes the design of a microwave interferometer developed at the LNS-INFN laboratories based on the so-called "frequency sweep" method to filter out the multipath contribution in the detected signals. The measurement technique and the preliminary results (calibration) obtained during the experimental tests will be presented.

  3. Microwave frequency sweep interferometer for plasma density measurements in ECR ion sources: Design and preliminary results.

    PubMed

    Torrisi, Giuseppe; Mascali, David; Neri, Lorenzo; Leonardi, Ornella; Sorbello, Gino; Celona, Luigi; Castro, Giuseppe; Agnello, Riccardo; Caruso, Antonio; Passarello, Santi; Longhitano, Alberto; Isernia, Tommaso; Gammino, Santo

    2016-02-01

    The Electron Cyclotron Resonance Ion Sources (ECRISs) development is strictly related to the availability of new diagnostic tools, as the existing ones are not adequate to such compact machines and to their plasma characteristics. Microwave interferometry is a non-invasive method for plasma diagnostics and represents the best candidate for plasma density measurement in hostile environment. Interferometry in ECRISs is a challenging task mainly due to their compact size. The typical density of ECR plasmas is in the range 10(11)-10(13) cm(-3) and it needs a probing beam wavelength of the order of few centimetres, comparable to the chamber radius. The paper describes the design of a microwave interferometer developed at the LNS-INFN laboratories based on the so-called "frequency sweep" method to filter out the multipath contribution in the detected signals. The measurement technique and the preliminary results (calibration) obtained during the experimental tests will be presented. PMID:26932081

  4. Extreme Energy Events Project: Construction of the detectors and installation in Italian High Schools

    NASA Astrophysics Data System (ADS)

    Abbrescia, M.; An, S.; Antolini, R.; Badalà, A.; Baldini Ferroli, R.; Bencivenni, G.; Blanco, F.; Bressan, E.; Chiavassa, A.; Chiri, C.; Cifarelli, L.; Cindolo, F.; Coccia, E.; de Pasquale, S.; di Giovanni, A.; D'Incecco, M.; Fabbri, F. L.; Frolov, V.; Garbini, M.; Gustavino, C.; Hatzifotiadou, D.; Imponente, G.; Kim, J.; La Rocca, P.; Librizzi, F.; Maggiora, A.; Menghetti, H.; Miozzi, S.; Moro, R.; Panareo, M.; Pappalardo, G. S.; Piragino, G.; Riggi, F.; Romano, F.; Sartorelli, G.; Sbarra, C.; Selvi, M.; Serci, S.; Williams, C.; Zichichi, A.; Zuyeuski, R.

    2008-04-01

    The EEE Project, conceived by its leader Antonino Zichichi, aims to detect Extreme Energy Events of cosmic rays with an array of muon telescopes distributed over the Italian territory. The Project involves Italian High Schools in order to introduce young people to Physics, also countervailing the recent crisis of university scientific classes inscriptions. The detectors for the EEE telescopes are Multigap Resistive Plate Chambers (MRPC) and have been constructed by teams of High School students who went in shift at the CERN laboratories. The mechanics and the electronics were developed by groups of researchers from CERN, the Italian Centro Fermi and INFN. The first group of schools of the EEE Project has inaugurated their telescopes recently. A status report of the Project and the preliminary results are presented.

  5. Computing on Knights and Kepler Architectures

    NASA Astrophysics Data System (ADS)

    Bortolotti, G.; Caberletti, M.; Crimi, G.; Ferraro, A.; Giacomini, F.; Manzali, M.; Maron, G.; Pivanti, M.; Salomoni, D.; Schifano, S. F.; Tripiccione, R.; Zanella, M.

    2014-06-01

    A recent trend in scientific computing is the increasingly important role of co-processors, originally built to accelerate graphics rendering, and now used for general high-performance computing. The INFN Computing On Knights and Kepler Architectures (COKA) project focuses on assessing the suitability of co-processor boards for scientific computing in a wide range of physics applications, and on studying the best programming methodologies for these systems. Here we present in a comparative way our results in porting a Lattice Boltzmann code on two state-of-the-art accelerators: the NVIDIA K20X, and the Intel Xeon-Phi. We describe our implementations, analyze results and compare with a baseline architecture adopting Intel Sandy Bridge CPUs.

  6. A high precision calorimeter for the SOX experiment

    NASA Astrophysics Data System (ADS)

    Papp, L.; Agostini, M.; Altenmüller, K.; Appel, S.; Caminata, A.; Cereseto, R.; Di Noto, L.; Farinon, S.; Musenich, R.; Neumair, B.; Oberauer, L.; Pallavicini, M.; Schönert, S.; Testera, G.; Zavatarelli, S.

    2016-07-01

    The SOX (Short distance neutrino Oscillations with BoreXino) experiment is being built to discover or reject eV-scale sterile neutrinos by observing short baseline oscillations of active-to-sterile neutrinos [1]. For this purpose, a 100 kCi 144Ce-144Pr antineutrino generator (CeSOX) will be placed under the BOREXINO detector at the Laboratori Nazionali del Gran Sasso. Thanks to its large size and very low background, BOREXINO is an ideal detector to discover or reject eV-scale sterile neutrinos. To reach the maximal sensitivity, we aim at determining the neutrino flux emitted by the antineutrino generator with a < 1 % accuracy. With this goal, TU München and INFN Genova are developing a vacuum calorimeter, which is designed to measure the source-generated heat with high accuracy.

  7. The XXVIII International Symposium on Lattice Field Theory

    NASA Astrophysics Data System (ADS)

    Lattice 2010, the XXVIII International Symposium on Lattice Field Theory, was held from June 14-19, 2010 at the ATAHOTEL Tanka Village Resort, Villasimius, Sardinia, Italy. The scientific programme contained 21 plenary session talks and over 300 parallel session contributions (talks and posters). The conference topics included: algorithms and machines, applications beyond QCD, chiral symmetry, hadron spectroscopy, hadronic structure and interactions, nonzero temperature and density, standard-model parameters and renormalization, theoretical developments, vacuum structure and confinement, weak decays and matrix elements. We would like to thank the members of International Advisory Committee for their help in planning the scientific programme. Thanks also go to all the speakers and delegates who helped to make the conference a big success. We acknowledge financial support from ICTP, INFN, the European Physical Journal, the European Community-Research Infrastructure Activity ``Hadron Physics 2", and the University of Rome ``Tor Vergata".

  8. The SIRAD irradiation facility at LNL

    SciTech Connect

    Bisello, D.; Giubilato, P.; Mattiazzo, S.; Pantano, D.; Silvestrin, L.; Candelori, A.; Tessaro, M.; Wyss, J.

    2013-07-18

    SIRAD is the irradiation facility at the Tandem XTU accelerator of the INFN National Laboratory of Legnaro (Padova, Italy) dedicated to study radiation effects, both comulative and Single Event, on silicon detectors and microelectronic devices and systems. SIRAD is routinely used by various research groups involved in the development of semiconductor detectors and electronics to be used in radiation hostile environments as experiments at high-energy/high luminosity accelerators or in space, or interested to study the basic radiation damage mechanisms. Single Events Effects (SEE) studies with micrometric resolution can be performed at SIRAD thanks to an Ion Electron Emission Microscope (IEEM) inserted at the end of the beam line. Here we describe the SIRAD facility including the available and future beam characteristics, and the IEEM performances as well as the scientific and technological solutions which have been implemented for its construction.

  9. Jefferson Laboratory Hall A SuperBigBite spectrometer GEM readout scheme

    NASA Astrophysics Data System (ADS)

    Camsonne, Alexandre; Hall A SuperBigBite Collaboration

    2015-10-01

    The SuperBigBite spectrometer at Jefferson Laboratory in Hall A will rely on Gas Electron Multiplier trackers to achieve the required luminosity for large momentum transfer experiments. The readout electronics is based on the APV25 ASIC and the INFN VME board Multi Purpose Digitizer (MPD) Board. By using the optical readout of readout of the boards, all boards can be readout in parallel effectively increasing the data bandwidth for one crate by more than a factor of 10. With such scheme the readout of several samples become possible and background rejection can be applied to reduce the large quantity of data due to the high occupancy of detector before it is recorded. This special readout scheme based on the JLab electronics will be presented along with the online data reduction strategy for the GEM data.

  10. The AGILE Data Center at ASDC

    NASA Astrophysics Data System (ADS)

    Pittori, Carlotta; AGILE Collaboration

    2013-01-01

    AGILE is a Scientific Mission of the Italian Space Agency (ASI) with INFN, INAF and CIFS participation, devoted to gamma-ray astrophysics. The satellite has been in orbit since April 23rd, 2007. Thanks to its sky monitoring capability and fast ground segment alert system, AGILE produced several important scientific results, among which was the unexpected discovery of strong and rapid gamma-ray flares from the Crab Nebula over daily timescales. This discovery won for the AGILE PI and the AGILE Team the Bruno Rossi Prize for 2012. The AGILE Data Center, located at ASDC, is in charge of all the scientific oriented activities related to the analysis and archiving of AGILE data. I will present the AGILE data center main activities, and I will give an overview of the AGILE scientific highlights after 5 years of operations.

  11. Optimisation of the usage of LHC and local computing resources in a multidisciplinary physics department hosting a WLCG Tier-2 centre

    NASA Astrophysics Data System (ADS)

    Barberis, Stefano; Carminati, Leonardo; Leveraro, Franco; Mazza, Simone Michele; Perini, Laura; Perlz, Francesco; Rebatto, David; Tura, Ruggero; Vaccarossa, Luca; Villaplana, Miguel

    2015-12-01

    We present the approach of the University of Milan Physics Department and the local unit of INFN to allow and encourage the sharing among different research areas of computing, storage and networking resources (the largest ones being those composing the Milan WLCG Tier-2 centre and tailored to the needs of the ATLAS experiment). Computing resources are organised as independent HTCondor pools, with a global master in charge of monitoring them and optimising their usage. The configuration has to provide satisfactory throughput for both serial and parallel (multicore, MPI) jobs. A combination of local, remote and cloud storage options are available. The experience of users from different research areas operating on this shared infrastructure is discussed. The promising direction of improving scientific computing throughput by federating access to distributed computing and storage also seems to fit very well with the objectives listed in the European Horizon 2020 framework for research and development.

  12. AGILE Data Center and AGILE science highlights

    NASA Astrophysics Data System (ADS)

    Pittori, C.

    2013-06-01

    AGILE is a scientific mission of the Italian Space Agency (ASI) with INFN, INAF e CIFS participation, devoted to gamma-ray astrophysics. The satellite is in orbit since April 23rd, 2007. Gamma-ray astrophysics above 100 MeV is an exciting field of astronomical sciences that has received a strong impulse in recent years. Despite the small size and budget, AGILE produced several important scientific results, among which the unexpected discovery of strong and rapid gamma-ray flares from the Crab Nebula. This discovery won to the AGILE PI and the AGILE Team the prestigious Bruno Rossi Prize for 2012, an international recognition in the field of high energy astrophysics. We present here the AGILE data center main activities, and we give an overview of the AGILE scientific highlights after 5 years of operations.

  13. Numerical investigation of beam-driven PWFA in quasi-nonlinear regime

    NASA Astrophysics Data System (ADS)

    Londrillo, P.; Gatti, C.; Ferrario, M.

    2014-03-01

    In beam-driven Plasma Based Wakefield Acceleration (PWFA), the quasi-nonlinear model has been designed to combine high efficient 'blowout' regimes, where cold and overdense driving electron beams form a totally rarefied plasma channel, with low charge beam distribution assuring the excited wakefield preserves relevant linear properties. This scheme can have applications in experimental facilities, like SPARC 150 MeV linac at LNF-INFN laboratories, where low-emittance, low-charge narrow electron beams can be produced to be injected on a preformed plasma channel. Here we present a preliminary numerical investigation of this configuration, using the fully 3D ALaDyn PIC code, as a preparatory work to design optimal conditions for the COMB experimental set-up. Specific numerical tools, having computational and diagnostic advantages in PWFA conditions and checks of the numerical outcomes with analytical results, are also presented and discussed.

  14. External-injection Experiment at SPARC_LAB

    NASA Astrophysics Data System (ADS)

    Rossi, A. R.; Bacci, A.; Belleveglia, M.; Chiadroni, E.; Cianchi, A.; Di Pirro, G.; Ferrario, M.; Gallo, A.; Gatti, G.; Maroli, C.; Mostacci, A.; Petrillo, V.; Serafini, L.; Tomassini, P.; Vaccarezza, C.

    At the SPARC_LAB facility of INFN-LNF we are installing two transport lines for ultra-short electron bunches and an ultra- intense laser pulses, generated by the SPARC photo-injector and by the FLAME laser in a synchronized fashion at the tens of f s level, to co-propagate inside a hydrogen filled glass capillary, in order to perform acceleration of the electron bunch by a plasma wave driven by the laser pulse. The main aim of this experiment is to demonstrate that a high brightness electron beam can be accelerated by a plasma wave without any significant degradation of its quality. Motivations of the technical choices made and expected performances are reported.

  15. The External-Injection experiment at the SPARC_LAB facility

    NASA Astrophysics Data System (ADS)

    Rossi, Andrea R.; Bacci, Alberto; Belleveglia, Marco; Chiadroni, Enrica; Cianchi, Alessandro; Di Pirro, Giampiero; Ferrario, Massimo; Gallo, Alessandro; Gatti, Giancarlo; Maroli, Cesare; Mostacci, Andrea; Petrillo, Vittoria; Serafini, Luca; Tomassini, Paolo; Vaccarezza, Cristina

    2014-03-01

    At the SPARC_LAB facility of INFN-LNF we are installing a transport lines for ultra-short electron bunches and another for ultra-intense laser pulses, generated by the SPARC photo-injector and by the FLAME laser in a synchronized fashion at the tens of fs level, to co-propagate inside a hydrogen filled glass capillary, in order to perform acceleration of the electron bunch by a plasma wave driven by the laser pulse. The main aim of this experiment is to demonstrate that a high brightness electron beam can be accelerated by a plasma wave without any significant degradation of its quality. Motivations of the technical choices are made and expected performances are reported.

  16. Thermal neutron detection using a silicon pad detector and {sup 6}LiF removable converters

    SciTech Connect

    Barbagallo, Massimo; Cosentino, Luigi; Marchetta, Carmelo; Pappalardo, Alfio; Scire, Carlotta; Scire, Sergio; Schillaci, Maria; Vecchio, Gianfranco; Finocchiaro, Paolo; Forcina, Vittorio; Peerani, Paolo; Vaccaro, Stefano

    2013-03-15

    A semiconductor detector coupled with a neutron converter is a good candidate for neutron detection, especially for its compactness and reliability if compared with other devices, such as {sup 3}He tubes, even though its intrinsic efficiency is rather lower. In this paper we show a neutron detector design consisting of a 3 cm Multiplication-Sign 3 cm silicon pad detector coupled with one or two external {sup 6}LiF layers, enriched in {sup 6}Li at 95%, placed in contact with the Si active surfaces. This prototype, first characterized and tested at INFN Laboratori Nazionali del Sud and then at JRC Ispra, was successfully shown to detect thermal neutrons with the expected efficiency and an outstanding gamma rejection capability.

  17. Thermo-mechanical characterization of insulated and epoxy-impregnated Nb3Sn composites

    SciTech Connect

    Linda Imbasciati et al.

    2002-12-11

    Nb{sub 3}Sn is, at present, the best superconductor for high field accelerator magnets. Several models using Nb{sub 3}Sn are under development in many laboratories. Knowledge of the thermo-mechanical properties of the impregnated coils is of crucial importance for the design of these magnets. In fact, the performance of epoxy-impregnated coils is sensitive to the thermal conductivity value, especially in case of heating caused by hysteretic losses, which are usually relevant in Nb{sub 3}Sn magnets, and in the case of continuous heat deposition, such as in magnets near the interaction region of a collider. Thermal contraction measurements are necessary to estimate the stresses during the magnet thermal cycle. Different insulation materials have been studied at Fermilab utilizing various design approaches and fabrication methods. Thermal conductivity and thermal contraction measurements, at cryogenic temperatures, have been performed respectively at INFN-LASA and Fermilab. The results are reported and discussed in this paper.

  18. Evaluation of Neutron Resonance Cross Section Data at GELINA

    NASA Astrophysics Data System (ADS)

    Schillebeeckx, P.; Becker, B.; Capote, R.; Emiliani, F.; Guber, K.; Heyse, J.; Kauwenberghs, K.; Kopecky, S.; Lampoudis, C.; Massimi, C.; Mondelaers, W.; Moxon, M.; Noguere, G.; Plompen, A. J. M.; Pronyaev, V.; Siegler, P.; Sirakov, I.; Trkov, A.; Volev, K.; Zerovnik, G.

    2014-05-01

    Over the last decade, the EC-JRC-IRMM, in collaboration with other institutes such as INRNE Sofia (BG), INFN Bologna (IT), ORNL (USA), CEA Cadarache (FR) and CEA Saclay (FR), has made an intense effort to improve the quality of neutron-induced cross section data in the resonance region. These improvements relate to both the infrastructure of the facility and the measurement setup, and the data reduction and analysis procedures. As a result total and reaction cross section data in the resonance region with uncertainties better than 0.5 % and 2 %, respectively, can be produced together with evaluated data files for both the resolved and unresolved resonance region. The methodology to produce full ENDF compatible files, including covariances, is illustrated by the production of resolved resonance parameter files for 241Am, Cd and W and an evaluation for 197Au in the unresolved resonance region.

  19. The Computing Grids

    NASA Astrophysics Data System (ADS)

    Govoni, P.

    2009-12-01

    Since the beginning of the millennium, High Energy Physics research institutions like CERN and INFN pioneered several projects aimed at exploiting the synergy among computing power, storage and network resources, and creating an infrastructure of distributed computing on a worldwide scale. In the year 2000, after the Monarch project [ http://monarc.web.cern.ch/MONARC/], DataGrid started [ http://eu-datagrid.web.cern.ch/eu-datagrid/] aimed at providing High Energy Physics with the computing power needed for the LHC enterprise. This program evolved into the EU DataGrid project, that implemented the first actual prototype of a Grid middleware running on a testbed environment. The next step consisted in the application to the LHC experiments, with the LCG project [ http://lcg.web.cern.ch/LCG/], in turn followed by the EGEE [ http://www.eu-egee.org/] and EGEE II programs.

  20. Characterization of the 80-mm diameter Hamamatsu PMTs for the KM3NeT project

    SciTech Connect

    Aiello, S.; Giordano, V.; Leonora, E.; Classen, L.; Reubelt, J.; Peek, H.; Visser, E.; Samtleben, D.; Kalekin, Oleg Collaboration: KM3NeT Collaboration

    2014-11-18

    The optical module designed for the KM3NeT project consists of 31 photomultipliers of 3-inch diameter housed into a 17-inch diameter glass sphere. A proposed photomultiplier was the R12199-02 Hamamatsu 80-mm diameter. 203 of such PMTs have been delivered from Hamamatsu and tested by the KM3NeT groups of NIKHEF-Amsterdam, ECAP-Erlangen and INFN-Catania. Tests have been performed to measure the main parameters, such as gain, transit time spread, dark pulses rate, fraction of spurious pulses, quantum efficiency and effective photocathode size. The main results matched with the requirements of the project. Methods and results are presented in this report.

  1. Neutron dosimetry at a high-energy electron-positron collider

    NASA Astrophysics Data System (ADS)

    Bedogni, Roberto

    Electron-positron colliders with energy of hundreds of MeV per beam have been employed for studies in the domain of nuclear and sub-nuclear physics. The typical structure of such a collider includes an LINAC, able to produce both types of particles, an accumulator ring and a main ring, whose diameter ranges from several tens to hundred meters and allows circulating particle currents of several amperes per beam. As a consequence of the interaction of the primary particles with targets, shutters, structures and barriers, a complex radiation environment is produced. This paper addresses the neutron dosimetry issues associated with the operation of such accelerators, referring in particular to the DAΦ NE complex, operative since 1997 at INFN-Frascati National Laboratory (Italy). Special attention is given to the active and passive techniques used for the spectrometric and dosimetric characterization of the workplace neutron fields, for radiation protection dosimetry purposes.

  2. Shedding new light on kaon-nucleon/nuclei interaction and its astrophysical implications with the AMADEUS experiment at DAΦNE

    NASA Astrophysics Data System (ADS)

    Scordo, A.; Bazzi, M.; Bellotti, G.; Berucci, C.; Bosnar, D.; Bragadireanu, A. M.; Clozza, A.; Cargnelli, M.; Curceanu, C.; Butt, A. Dawood; Grande, R. Del; Fabbietti, L.; Fiorini, C.; Ghio, F.; Guaraldo, C.; Iliescu, M.; Sandri, P. Levi; Marton, J.; Pietreanu, D.; Piscicchia, K.; Shi, H.; Sirghi, D.; Sirghi, F.; Tucakovic, I.; Doce, O. Vazquez; Wiedmann, W.; Zmeskal, J.

    2016-05-01

    The AMADEUS experiment deals with the investigation of the low-energy kaon-nuclei hadronic interaction at the DAΦNE collider at LNF-INFN. The aim is to answer fundamental longstanding questions in the non-perturbative QCD strangeness sector. The antikaon-nucleon potential is investigated searching for signals from possible bound kaonic clusters, which would open the possibility for the formation of cold dense baryonic matter. The confirmation of this scenario may imply a fundamental role of strangeness in astrophysics. AMADEUS step 0 consisted in the reanalysis of 2004/2005 KLOE dataset, exploiting K- absorptions in H, 4He, 9Be and 12C in the setup materials. In this paper, together with a review on the multi-nucleon K- absorption and the particle identification procedure, the first results on the Σ0p channel will be presented including a statistical analysis on the possible accomodation of a deeply bound state.

  3. Response of microchannel plates to single particles and to electromagnetic showers

    NASA Astrophysics Data System (ADS)

    Brianza, L.; Cavallari, F.; Del Re, D.; Gelli, S.; Ghezzi, A.; Gotti, C.; Govoni, P.; Jorda Lopez, C.; Martelli, A.; Marzocchi, B.; Meridiani, P.; Organtini, G.; Paramatti, R.; Pernié, L.; Pigazzini, S.; Rahatlou, S.; Rovelli, C.; Santanastasio, F.; Tabarelli de Fatis, T.; Trevisani, N.

    2015-10-01

    We report on the response of microchannel plates (MCPs) to single relativistic particles and to electromagnetic showers. Particle detection by means of secondary emission of electrons at the MCP surface has long been proposed and is used extensively in ion time-of-flight mass spectrometers. What has not been investigated in depth is their use to detect the ionizing component of showers. The time resolution of MCPs exceeds anything that has been previously used in calorimeters and, if exploited effectively, could aid in the event reconstruction at high luminosity colliders. Several prototypes of photodetectors with the amplification stage based on MCPs were exposed to cosmic rays and to 491 MeV electrons at the INFN-LNF Beam-Test Facility. The time resolution and the efficiency of the MCPs are measured as a function of the particle multiplicity, and the results used to model the response to high-energy showers.

  4. Accurate on line measurements of low fluences of charged particles

    NASA Astrophysics Data System (ADS)

    Palla, L.; Czelusniak, C.; Taccetti, F.; Carraresi, L.; Castelli, L.; Fedi, M. E.; Giuntini, L.; Maurenzig, P. R.; Sottili, L.; Taccetti, N.

    2015-03-01

    Ion beams supplied by the 3MV Tandem accelerator of LABEC laboratory (INFN-Firenze), have been used to study the feasibility of irradiating materials with ion fluences reproducible to about 1%. Test measurements have been made with 7.5 MeV 7Li2+ beams of different intensities. The fluence control is based on counting ions contained in short bursts generated by chopping the continuous beam with an electrostatic deflector followed by a couple of adjustable slits. Ions are counted by means of a micro-channel plate (MCP) detecting the electrons emitted from a thin layer of Al inserted along the beam path in between the pulse defining slits and the target. Calibration of the MCP electron detector is obtained by comparison with the response of a Si detector.

  5. Mu2e transport solenoid prototype tests results

    DOE PAGESBeta

    Lopes, Mauricio L.; G. Ambrosio; DiMarco, J.; Evbota, D.; Feher, S.; Friedsam, H.; Galt, A.; Hays, S.; Hocker, J.; Kim, M. J.; et al

    2016-02-08

    The Fermilab Mu2e experiment has been developed to search for evidence of charged lepton flavor violation through the direct conversion of muons into electrons. The transport solenoid is an s-shaped magnet which guides the muons from the source to the stopping target. It consists of fifty-two superconducting coils arranged in twenty-seven coil modules. A full-size prototype coil module, with all the features of a typical module of the full assembly, was successfully manufactured by a collaboration between INFN-Genoa and Fermilab. The prototype contains two coils that can be powered independently. In order to validate the design, the magnet went throughmore » an extensive test campaign. Warm tests included magnetic measurements with a vibrating stretched wire, electrical and dimensional checks. As a result, the cold performance was evaluated by a series of power tests as well as temperature dependence and minimum quench energy studies.« less

  6. The virtual organization membership service extention project (VOX)

    SciTech Connect

    Levshina, T.; Bauderick, L.; Berman, E.; Fisk, I.; Graham, G.; Heavey, A.; Kaiser, J.; Pordes, R.; Sekhri, V.; Skow, D.; Weigand, J.; Wu, Y.; Baker, R.; Carcassi, G.; Gardner, R.; Grundhoefer, L.; /Indiana U.

    2004-12-01

    Current grid development projects are being designed such that they require end users to be authenticated under the auspices of a ''recognized'' organization, called a Virtual Organization (VO). A VO must establish resource-usage agreements with grid resource providers. The VO is responsible for authorizing its members for grid computing privileges. The individual sites and resources typically enforce additional layers of authorization. The VOX project developed at Fermilab is an extension of VOMS, developed jointly for DataTAG by INFN and for DataGrid by CERN. The VOX project provides set of services that facilitate grid users registration with a VO, administration of VO members, as well as control access of grid users to a particular site. The current state of deployment and future steps to improve the prototype and implement some new features will be discussed.

  7. Online monitoring for the CDF Run II experiment and the remote operation facilities

    SciTech Connect

    Arisawa, T.; Fabiani, D.; Hirschbuehl, D.; Ikado, K.; Kubo, T.; Kusakabe, Y.; Maeshima, K.; Naganoma, J.; Nakamura, K.; Plager, C.; Schmidt, E.; /Fermilab /INFN, Pisa /Karlsruhe U.

    2007-01-01

    The foundation of the CDF Run II online event monitoring framework, placed well before the physics runs start, allowed to develop coherent monitoring software across all the different subsystems which consequently made maintenance and operation simple and efficient. Only one shift person is needed to monitor the entire CDF detector, including the trigger system. High data quality check is assured in real time and well defined monitoring results are propagated coherently to offline datasets used for physics analyzes. We describe the CDF Run II online event monitoring system and operation, with emphasis on the remote monitoring shift operation started since November 2006 with Pisa-INFN as pilot Institution and exploiting the WEB based access to the data.

  8. Clustering microcalcifications techniques in digital mammograms

    NASA Astrophysics Data System (ADS)

    Díaz, Claudia. C.; Bosco, Paolo; Cerello, Piergiorgio

    2008-11-01

    Breast cancer has become a serious public health problem around the world. However, this pathology can be treated if it is detected in early stages. This task is achieved by a radiologist, who should read a large amount of mammograms per day, either for a screening or diagnostic purpose in mammography. However human factors could affect the diagnosis. Computer Aided Detection is an automatic system, which can help to specialists in the detection of possible signs of malignancy in mammograms. Microcalcifications play an important role in early detection, so we focused on their study. The two mammographic features that indicate the microcalcifications could be probably malignant are small size and clustered distribution. We worked with density techniques for automatic clustering, and we applied them on a mammography CAD prototype developed at INFN-Turin, Italy. An improvement of performance is achieved analyzing images from a Perugia-Assisi Hospital, in Italy.

  9. States of {sup 15}C via the ({sup 18}O,{sup 16}O) reaction

    SciTech Connect

    Cappuzzello, F.; Carbone, D.; Cavallaro, M.; Cunsolo, A.; Orrigo, S. E. A.; Foti, A.; Rodrigues, M. R. D.

    2010-04-30

    A study of the {sup 15}C states was pursued in 2008 at the Catania INFN-LNS laboratory by the {sup 13}C({sup 18}O,{sup 16}O){sup 15}C reaction at 84 MeV incident energy. The {sup 16}O ejectiles were detected at forward angles by the MAGNEX magnetic spectrometer. The spectra show several known low lying states up to about 7 MeV excitation energy as well as two unknown resonant structures at about 11.4 and 13.5 MeV. The strong excitation of these latter together with the measured width of about 2 MeV FWHM could indicate the presence of collective modes of excitation connected to the transfer of a correlated neutron pair.

  10. BaBar superconducting coil: design, construction and test

    SciTech Connect

    Bell, R A; Berndt, M; Burgess, W; Craddock, W; Dormicchi, O; Fabbricatore, P; Farinon, S; Keller, L; Moreschi, P; Musenich, R; O'Connor, T G; Penco, R; Priano, C; Shen, S; Valente, P

    2001-01-26

    The BABAR Detector, located in the PEP-II B-Factory at the Stanford Linear Accelerator Center, includes a large 1.5 Tesla superconducting solenoid, 2.8 m bore and length 3.7 m. The two layer solenoid is wound with an aluminum stabilized conductor which is graded axially to produce a {+-} 3% field uniformity in the tracking region. This paper summarizes the 3 year design, fabrication and testing program of the superconducting solenoid. The work was carried out by an international collaboration between INFN, LLNL and SLAC. The coil was constructed by Ansaldo Energia. Critical current measurements of the superconducting strand, cable and conductor, cool-down, operation with the thermo-siphon cooling, fast and slow discharges, and magnetic forces are discussed in detail.

  11. First samples of Ti and Nb tubes explosion welding joint with stainless steel for ILC 1.8 K cryomodule

    NASA Astrophysics Data System (ADS)

    Sabirov, B. M.; Budagov, J. A.; Shirkov, G. D.

    2013-07-01

    The world first samples of Ti and Nb tubes joint with stainless steel ones by an explosion welding by the JINR-VNIIEF-FNAL-INFN cooperation were manufactured in the frame of ILC R&D programe. An applying methods of relaxation of residual tensions (after explosion and electron beam welding), macro- and microanalyses of welding seam and cryogenic tests of the samples produced manifest the achievement of high mechanic strength (≈250 MPa/share) of welding seam, solidity and leak absence on 10-10 l atm/s level at 1.8 K. The explosion welding technology and methods introducing to industrial manufacturing of the 4-th generation of cryomodule of TESLA TYPE DESIGN can exclude the Ti—communications, connect the Nb—cavity with stainless steel vessel and reduce significantly the accelerator cost.

  12. Underground nuclear astrophysics: Why and how

    NASA Astrophysics Data System (ADS)

    Best, A.; Caciolli, A.; Fülöp, Zs.; Gyürky, Gy.; Laubenstein, M.; Napolitani, E.; Rigato, V.; Roca, V.; Szücs, T.

    2016-04-01

    The goal of nuclear astrophysics is to measure cross-sections of nuclear physics reactions of interest in astrophysics. At stars temperatures, these cross-sections are very low due to the suppression of the Coulomb barrier. Cosmic-ray-induced background can seriously limit the determination of reaction cross-sections at energies relevant to astrophysical processes and experimental setups should be arranged in order to improve the signal-to-noise ratio. Placing experiments in underground sites, however, reduces this background opening the way towards ultra low cross-section determination. LUNA (Laboratory for Underground Nuclear Astrophysics) was pioneer in this sense. Two accelerators were mounted at the INFN National Laboratories of Gran Sasso (LNGS) allowing to study nuclear reactions close to stellar energies. A summary of the relevant technology used, including accelerators, target production and characterisation, and background treatment is given.

  13. Recent developments of ion beam induced luminescence at the external scanning microbeam facility of the LABEC laboratory in Florence

    NASA Astrophysics Data System (ADS)

    Colombo, E.; Calusi, S.; Cossio, R.; Giuntini, L.; Giudice, A. Lo; Mandò, P. A.; Manfredotti, C.; Massi, M.; Mirto, F. A.; Vittone, E.

    2008-04-01

    A new ionoluminescence (IL) apparatus has been successfully installed at the external scanning microbeam facility of the 3 MV Tandetron accelerator of the INFN LABEC in Firenze; the apparatus for photon detection has been fully integrated in the existing ion beam analysis (IBA) set-up, for the simultaneous acquisition of IL and PIXE/PIGE/BS spectra and maps. The potential of the new set-up is illustrated in this paper by some results extracted by the analysis of art objects and advanced semiconductor materials. In particular, the adequacy of the new IBA set-up in the field of cultural heritage is pointed out by the coupled PIXE/IL micro-analysis of a lapis lazuli stone; concerning applications in material science, IL spectra from a N doped diamond sample were acquired and compared with CL analyses to evaluate the relevant sensitivities and the effect of ion damage.

  14. Salerno Exultet: its characterization by Raman and PIXE-α analyses

    NASA Astrophysics Data System (ADS)

    Bicchieri, Marina; Nardone, Michele; Pappalardo, Giuseppe; Pappalardo, Lighea; Romano, Francesco P.; Russo, Pio A.; Sodo, Armida

    2001-10-01

    Two non-destructive techniques, Raman spectroscopy and particle induced x-ray emission spectrometry, were used for a compete connotation of the Salerno Exultet, an illuminated parchment dated back to 13th century. The Salerno Exultet exhibits several areas which have underdone restoration treatments during the years. The complete connotation of this masterpiece was necessary in order to identify and to distinguish the original and the added pigments. Owing to its dimensions, it has been impossible to analyze the Salerno Exultet directly under the microscope of our Micro- Raman spectrometer. We have therefore used optical fibers connected to a three axis micro-positioned measurement head mounted on a large metal frame. PIXE analysis was performed using the portable instrumentation set up by the INFN-LANDIS Laboratory, Catania, Italy. The combination of these two complementary techniques allowed us to reveal a palette formed by lapis-lazuli, cinnabar, verdigris, earths organic lakes and chalk.

  15. IXPE - The Imaging X-Ray Polarimetry Explorer

    NASA Technical Reports Server (NTRS)

    Ramsey, Brian

    2014-01-01

    The Imaging X-ray Polarimetry Explorer (IXPE) is a Small Explorer Mission that will be proposed in response to NASA's upcoming Announcement of Opportunity. IXPE will transform our understanding of the most energetic and exotic astrophysical objects, especially neutron stars and black holes, by measuring the linear polarization of astronomical objects as a function of energy, time and, where relevant, position. As the first dedicated polarimetry observatory IXPE will add a new dimension to the study of cosmic sources, enlarging the observational phase space and providing answers to fundamental questions. IXPE will feature x-ray optics fabricated at NASA/MSFC and gas pixel focal plane detectors provided by team members in Italy (INAF and INFN). This presentation will give an overview of the proposed IXPE mission, detailing the payload configuration, the expected sensitivity, and a typical observing program.

  16. LYSO crystal calorimeter readout with silicon photomultipliers

    NASA Astrophysics Data System (ADS)

    Berra, A.; Bonvicini, V.; Cecchi, C.; Germani, S.; Guffanti, D.; Lietti, D.; Lubrano, P.; Manoni, E.; Prest, M.; Rossi, A.; Vallazza, E.

    2014-11-01

    Large area Silicon PhotoMultipliers (SiPMs) are the new frontier of the development of readout systems for scintillating detectors. A SiPM consists of a matrix of parallel-connected silicon micropixels operating in limited Geiger-Muller avalanche mode, and thus working as independent photon counters with a very high gain (~106). This contribution presents the performance in terms of linearity and energy resolution of an electromagnetic homogeneous calorimeter composed of 9 ~ 18X0 LYSO crystals. The crystals were readout by 36 4×4 mm2 SiPMs (4 for each crystal) produced by FBK-irst. This calorimeter was tested at the Beam Test Facility at the INFN laboratories in Frascati with a single- and multi-particle electron beam in the 100-500 MeV energy range.

  17. RF Heating in Electron Cyclotron Resonance Ion Sources

    NASA Astrophysics Data System (ADS)

    Mascali, D.; Gammino, S.; Celona, L.; Ciavola, G.

    2011-12-01

    ECRIS—Electron Cyclotron Resonance Ion Sources are able to feed accelerators with intense currents of highly charged ions. In ECRIS a high density—high temperature plasma is generated by means of the Electron Cyclotron Resonance Heating inside a B-min, MHD stable trap. The state of the art about the principal heating mechanisms will be given. The paper will specially discuss the most critical and still open issues concerning the influence of the magnetic field and of the RF frequency on the plasma heating, as well as the impact of possible non-linear pumping wave—to—plasma interactions. The contribution of INFN-LNS will be specifically underlined. A short review on the future perspectives for the design of new generation ion sources will be given in conclusion.

  18. EEE - Extreme Energy Events: an astroparticle physics experiment in Italian High Schools

    NASA Astrophysics Data System (ADS)

    Abbrescia, M.; Avanzini, C.; Baldini, L.; Baldini Ferroli, R.; Batignani, G.; Bencivenni, G.; Bossini, E.; Bressan, E.; Chiavassa, A.; Cicalò, C.; Cifarelli, L.; Coccia, E.; Corvaglia, A.; De Gruttola, D.; De Pasquale, S.; Di Giovanni, A.; D’Incecco, M.; Dreucci, M.; Fabbri, F. L.; Fattibene, E.; Ferrarov, A.; Forster, R.; Frolov, V.; Galeotti, P.; Garbini, M.; Gemme, G.; Gnesi, I.; Grazzi, S.; Gustavino, C.; Hatzifotiadou, D.; La Rocca, P.; Maggiora, A.; Maron, G.; Mazziotta, M. N.; Miozzi, S.; Noferini, F.; Nozzoli, F.; Panareo, M.; Panetta, M. P.; Paoletti, R.; Perasso, L.; Pilo, F.; Piragino, G.; Riggi, F.; Righini, G. C.; Rodriguez Rodriguez, A.; Sartorelli, G.; Scapparone, E.; Schioppa, M.; Scribano, A.; Selvi, M.; Serci, S.; Siddi, E.; Squarcia, S.; Taiuti, M.; Terreni, G.; Vistoli, M. C.; Votano, L.; Williams, M. C. S.; Zani, S.; Zichichi, A.; Zuyeuski, R.

    2016-05-01

    The Extreme Energy Events project (EEE) is aimed to study Extensive Air Showers (EAS) from primary cosmic rays of more than 1018 eV energy detecting the ground secondary muon component using an array of telescopes with high spatial and time resolution. The second goal of the EEE project is to involve High School teachers and students in this advanced research work and to initiate them in scientific culture: to reach both purposes the telescopes are located inside High School buildings and the detector construction, assembling and monitoring - together with data taking and analysis - are done by researchers from scientific institutions in close collaboration with them. At present there are 42 telescopes in just as many High Schools scattered all over Italy, islands included, plus two at CERN and three in INFN units. We report here some preliminary physics results from the first two common data taking periods together with the outreach impact of the project.

  19. Plasma density characterization at SPARC_LAB through Stark broadening of Hydrogen spectral lines

    NASA Astrophysics Data System (ADS)

    Filippi, F.; Anania, M. P.; Bellaveglia, M.; Biagioni, A.; Chiadroni, E.; Cianchi, A.; Di Giovenale, D.; Di Pirro, G.; Ferrario, M.; Mostacci, A.; Palumbo, L.; Pompili, R.; Shpakov, V.; Vaccarezza, C.; Villa, F.; Zigler, A.

    2016-09-01

    Plasma-based acceleration techniques are of great interest for future, compact accelerators due to their high accelerating gradient. Both particle-driven and laser-driven Plasma Wakefield Acceleration experiments are foreseen at the SPARC_LAB Test Facility (INFN National Laboratories of Frascati, Italy), with the aim to accelerate high-brightness electron beams. In order to optimize the efficiency of the acceleration in the plasma and preserve the quality of the accelerated beam, the knowledge of the plasma electron density is mandatory. The Stark broadening of the Hydrogen spectral lines is one of the candidates used to characterize plasma density. The implementation of this diagnostic for plasma-based experiments at SPARC_LAB is presented.

  20. 3D-full wave and kinetics numerical modelling of electron cyclotron resonance ion sources plasma: steps towards self-consistency

    NASA Astrophysics Data System (ADS)

    Mascali, David; Torrisi, Giuseppe; Neri, Lorenzo; Sorbello, Gino; Castro, Giuseppe; Celona, Luigi; Gammino, Santo

    2015-01-01

    Electron Cyclotron Resonance (ECR) ion Sources are the most performing machines for the production of intense beams of multi-charged ions in fundamental science, applied physics and industry. Investigation of plasma dynamics in ECRIS still remains a challenge. A better comprehension of electron heating, ionization and diffusion processes, ion confinement and ion beam formation is mandatory in order to increase ECRIS performances both in terms of output beams currents, charge states, beam quality (emittance minimization, beam halos suppression, etc.). Numerical solution of Vlasov equation via kinetic codes coupled to FEM solvers is ongoing at INFN-LNS, based on a PIC strategy. Preliminary results of the modeling will be shown about wave-plasma interaction and electron-ion confinement: the obtained results are very helpful to better understand the influence of the different parameters (especially RF frequency and power) on the ion beam formation mechanism.

  1. Proof of concept of an imaging system demonstrator for PET applications with SiPM

    NASA Astrophysics Data System (ADS)

    Morrocchi, Matteo; Marcatili, Sara; Belcari, Nicola; Giuseppina Bisogni, Maria; Collazuol, Gianmaria; Ambrosi, Giovanni; Santoni, Cristiano; Corsi, Francesco; Foresta, Maurizio; Marzocca, Cristoforo; Matarrese, Gianvito; Sportelli, Giancarlo; Guerra, Pedro; Santos, Andres; Del Guerra, Alberto

    2013-08-01

    A PET imaging system demonstrator based on LYSO crystal arrays coupled to SiPM matrices is under construction at the University and INFN of Pisa. Two SiPM matrices, composed of 8×8 SiPM pixels, and 1,5 mm pitch, have been coupled one to one to a LYSO crystals array and read out by a custom electronics system. front-end ASICs were used to read 8 channels of each matrix. Data from each front-end were multiplexed and sent to a DAQ board for the digital conversion; a motherboard collects the data and communicates with a host computer through a USB port for the storage and off-line data processing. In this paper we show the first preliminary tomographic image of a point-like radioactive source acquired with part of the two detection heads in time coincidence.

  2. On-line test using multi-foil SiC target at iThemba LABS

    NASA Astrophysics Data System (ADS)

    Monetti, A.; Bark, R. A.; Andrighetto, A.; Beukes, P.; Conradie, J. L.; Corradetti, S.; Fourie, D.; Lussi, C.; Manzolaro, M.; Meneghetti, G.; Prete, G.; Rossignoli, M.; Scarpa, D.; Van Schalkwyk, P.; Stoddart, N.; Vasquez, J.

    2016-06-01

    In a collaboration between the INFN-SPES project and iThemba LABS, an on-line test of the power dissipation of a multi-slice target concept for radioactive-ion beam production has been performed. Using the 66MeV proton beam from the iThemba LABS Separated Sector Cyclotron, a total power of 4kW was deposited in the target complex, consisting of 13 Silicon Carbide (SiC) discs of about 1 millimetre thickness housed in a graphite cylinder. The results of the measurements fully validate thermal FEM simulations and confirm that the multi-foil target system is a reliable and affordable device for second-generation ISOL-RIB facilities like SPES.

  3. Background modeling for the GERDA experiment

    SciTech Connect

    Becerici-Schmidt, N.; Collaboration: GERDA Collaboration

    2013-08-08

    The neutrinoless double beta (0νββ) decay experiment GERDA at the LNGS of INFN has started physics data taking in November 2011. This paper presents an analysis aimed at understanding and modeling the observed background energy spectrum, which plays an essential role in searches for a rare signal like 0νββ decay. A very promising preliminary model has been obtained, with the systematic uncertainties still under study. Important information can be deduced from the model such as the expected background and its decomposition in the signal region. According to the model the main background contributions around Q{sub ββ} come from {sup 214}Bi, {sup 228}Th, {sup 42}K, {sup 60}Co and α emitting isotopes in the {sup 226}Ra decay chain, with a fraction depending on the assumed source positions.

  4. Accelerator-based analytical technique in the evaluation of some Nigeria’s natural minerals: Fluorite, tourmaline and topaz

    NASA Astrophysics Data System (ADS)

    Olabanji, S. O.; Ige, O. A.; Mazzoli, C.; Ceccato, D.; Akintunde, J. A.; De Poli, M.; Moschini, G.

    2005-10-01

    For the first time, the complementary accelerator-based analytical technique of PIXE and electron microprobe analysis (EMPA) were employed for the characterization of some Nigeria's natural minerals namely fluorite, tourmaline and topaz. These minerals occur in different areas in Nigeria. The minerals are mainly used as gemstones and for other scientific and technological applications and therefore are very important. There is need to characterize them to know the quality of these gemstones and update the geochemical data on them geared towards useful applications. PIXE analysis was carried out using the 1.8 MeV collimated proton beam from the 2.5 MV AN 2000 Van de Graaff accelerator at INFN, LNL, Legnaro, Padova, Italy. The novel results which show many elements at different concentrations in these minerals are presented and discussed.

  5. Particle Dark Matter in the galactic halo: results from DAMA/LIBRA

    SciTech Connect

    Bernabei, R.; Belli, P.; Nozzoli, F.; Montecchia, F.; Cappella, F.; D'Angelo, A.; Incicchitti, A.; Presperi, D.; Cerulli, R.; Dai, C. J.; He, H. L.; Kuang, H. H.; Ma, X. H.; Sheng, X. D.

    2010-02-10

    The DAMA/LIBRA experiment at the Gran Sasso National Laboratory of the I.N.F.N. has confirmed with higher sensitivity the model independent evidence for Dark Matter (DM) particles in the galactic halo obtained by the former DAMA/NaI experiment by investigating the DM annual modulation signature. Considering the data collected by DAMA/LIBRA together with the data collected by the former DAMA/NaI (cumulative exposure of 0.82 tonxyr) a confidence level of 8.2 sigma has been achieved. The experiment is in data taking; a first upgrading of the set-up has been carried out in Spetember 2008 and a second one--aiming to decrease the experimental energy threshold--is foreseen in September 2010.

  6. Site Studies for the SuperB Collider and Synchrotron Radiation Facility Project

    SciTech Connect

    Tomassini, S.; Biagini, M.E.; Raimondi, P.; Sanelli, C.; Bolzon, B.; Deleglise, G.; Jeremie, A.; Seeman, J.; /SLAC

    2012-04-26

    The SuperB complex project aims at the construction of a very high luminosity (10{sup 36} cm{sup -2}s{sup -1}) asymmetric e{sup +}e{sup -} B-factory and a few X-ray synchrotron beam lines (SR). The project has been recently approved by the Italian Government as part of the National Research Plan. The Tor Vergata University location has been chosen and a Consortium among INFN, University of Rome II Tor Vergata and the Research Ministry is being signed, allowing for the constitution of the 'Cabibbo Laboratory', where the SuperB project will be hosted. This paper presents and describes the status of the preliminary design of the site layout, related issues for the chosen site and the preliminary ground motion (GM) measurement results.

  7. SPECIAL SEMINAR - The NOTTE experiment, or how to become a Total Solar Eclipse chaser

    SciTech Connect

    2011-02-08

    The NOTTE experiment (Neutrino Oscillations with Telescope during Total Eclipse) aims at searching for visible photons emitted through a possible radiative decay of solar neutrinos. The experiment and the expeditions organized by a group of physicists and astrophysicists from INFN and INAF Bologna hunting for Total Solar Eclipses from 1998 to 2006 wil be described. The results of observations performed during total solar eclipse expeditions in 2001 (Zambia) and 2006 (Sahara desert, Libya) are presented and a beautiful photo gallery will be shown. Other peculiar observations that can be made during a solar eclipse are also illustrated. The seminar will be followed by a brief presentation of future camps for solar eclipse chasers and scientists organized in 2008 in Russia, Kazakhstan, China and Mongolia, in 2009 in Shanghai and on the Easter Island in 2010.

  8. Ion beams 12, Legnaro 6-8 June 2012, the 50 years (1961-2011) of the Legnaro Laboratory

    SciTech Connect

    Ricci, Renato Angelo

    2013-07-18

    A short review of the history of the Legnaro Laboratory is presented since its foundation 50 years ago by Prof. A. Rostagni of the University of Padova. The evolution of the Laboratory as a national reference center for fundamental and applied nuclear physics researches is outlined, pointing out its transformation into the INFN National Laboratories in 1968. After the first CN VdG Accelerator of 5.5 MV operating in 1961 and the AN2000 devoted to interdisciplinary researches (1971), i.e. 40 years ago, ten years later the advent of the first heavy ion facility in Italy, the XTU Tandem accelerator, and later on of the ALPI superconducting linear accelerator, was crucial for any future developments, not only in the field of nuclear physics but also for the evolution of interdisciplinary programmes with ion beams.

  9. DAMA/LIBRA-phase1 results and perspectives of the phase2

    NASA Astrophysics Data System (ADS)

    Bernabei, R.; Belli, P.; Cappella, F.; Caracciolo, V.; Cerulli, R.; Dai, C. J.; d'Angelo, A.; d'Angelo, S.; Di Marco, A.; He, H. L.; Incicchitti, A.; Kuang, H. H.; Ma, X. H.; Montecchia, F.; Sheng, X. D.; Wang, R. G.; Ye, Z. P.

    2016-07-01

    The DAMA/LIBRA experiment (˜ 250 kg of highly radio-pure NaI(Tl)) is running deep underground at the Gran Sasso National Laboratory (LNGS) of the I.N.F.N. Here we briefly recall the results obtained in its first phase of measurements (DAMA/LIBRA-phase1; total exposure: 1.04 ton × yr). DAMA/LIBRA-phase1 and the former DAMA/NaI (cumulative exposure: 1.33 ton × yr) give evidence at 9.3 σ C.L. for the presence of DM particles in the galactic halo by exploiting the model-independent DM annual modulation signature. No systematic or side reaction able to mimic the exploited DM signature has been found or suggested by anyone over more than a decade. At present DAMA/LIBRA-phase2 is running with increased sensitivity.

  10. A new H2+ source: Conceptual study and experimental test of an upgraded version of the VIS—Versatile ion source

    NASA Astrophysics Data System (ADS)

    Castro, G.; Torrisi, G.; Celona, L.; Mascali, D.; Neri, L.; Sorbello, G.; Leonardi, O.; Patti, G.; Castorina, G.; Gammino, S.

    2016-08-01

    The versatile ion source is an off-resonance microwave discharge ion source which produces a slightly overdense plasma at 2.45 GHz of pumping wave frequency extracting more than 60 mA proton beams and 50 mA He+ beams. DAEδALUS and IsoDAR experiments require high intensities for H2+ beams to be accelerated by high power cyclotrons for neutrinos generation. In order to fulfill the new requirements, a new plasma chamber and injection system has been designed and manufactured for increasing the H2+ beam intensity. In this paper the studies for the increasing of the H2+/p ratio and for the design of the new plasma chamber and injection system will be shown and discussed together with the experimental tests carried out at Istituto Nazionale di Fisica Nucleare-Laboratori Nazionali del Sud (INFN-LNS) and at Best Cyclotron Systems test-bench in Vancouver, Canada.

  11. A steady-state high-temperature method for measuring thermal conductivity of refractory materials.

    PubMed

    Manzolaro, M; Corradetti, S; Andrighetto, A; Ferrari, L

    2013-05-01

    A new methodology and an instrumental setup for the thermal conductivity estimation of isotropic bulk graphite and different carbides at high temperatures are presented. The method proposed in this work is based on the direct measurement of temperature and emissivity on the top surface of a sample disc of known dimensions. Temperatures measured under steady-state thermal equilibrium are then used to estimate the thermal conductivity of the sample by making use of the inverse parameter estimation technique. Thermal conductivity values obtained in this way are then compared to the material data sheets and values found in literature. The reported work has been developed within the Research and Development framework of the SPES (Selective Production of Exotic Species) project at INFN-LNL (Istituto Nazionale di Fisica Nucleare - Laboratori Nazionali di Legnaro). PMID:23742578

  12. GPCALMA: implementation in Italian hospitals of a computer aided detection system for breast lesions by mammography examination.

    PubMed

    Lauria, Adele

    2009-06-01

    We describe the implementation in several Italian hospitals of a computer aided detection (CAD) system, named GPCALMA (grid platform for a computer aided library in mammography), for the automatic search of lesions in X-ray mammographies. GPCALMA has been under development since 1999 by a community of physicists of the Italian National Institute for Nuclear Physics (INFN) in collaboration with radiologists. This CAD system was tested as a support to radiologists in reading mammographies. The main system components are: (i) the algorithms implemented for the analysis of digitized mammograms to recognize suspicious lesions, (ii) the database of digitized mammographic images, and (iii) the PC-based digitization and analysis workstation and its user interface. The distributed nature of data and resources and the prevalence of geographically remote users suggested the development of the system as a grid application: the design of this networked version is also reported. The paper describes the system architecture, the database of digitized mammographies, the clinical workstation and the medical applications carried out to characterize the system. A commercial CAD was evaluated in a comparison with GPCALMA by analysing the medical reports obtained with and without the two different CADs on the same dataset of images: with both CAD a statistically significant increase in sensitivity was obtained. The sensitivity in the detection of lesions obtained for microcalcification and masses was 96% and 80%, respectively. An analysis in terms of receiver operating characteristic (ROC) curve was performed for massive lesion searches, achieving an area under the ROC curve of A(z)=0.783+/-0.008. Results show that the GPCALMA CAD is ready to be used in the radiological practice, both for screening mammography and clinical studies. GPCALMA is a starting point for the development of other medical imaging applications such as the CAD for the search of pulmonary nodules, currently under

  13. ELIMED, future hadrontherapy applications of laser-accelerated beams

    NASA Astrophysics Data System (ADS)

    Cirrone, Giuseppe A. P.; Carpinelli, Massimo; Cuttone, Giacomo; Gammino, Santo; Bijan Jia, S.; Korn, Georg; Maggiore, Mario; Manti, Lorenzo; Margarone, Daniele; Prokupek, Jan; Renis, Marcella; Romano, Francesco; Schillaci, Francesco; Tomasello, Barbara; Torrisi, Lorenzo; Tramontana, Antonella; Velyhan, Andriy

    2013-12-01

    Laser-ion acceleration has recently gained a great interest as an alternative to conventional and more expensive acceleration techniques. These ion beams have desirable qualities such as small source size, high luminosity and small emittance to be used in different fields as Nuclear Physics, Medical Physics, etc. This is very promising specially for the future perspective of a new concept of hadrontherapy based on laser-based devices could be developed, replacing traditional accelerating machines. Before delivering laser-driven beams for treatments they have to be handled, cleaned from unwanted particles and characterized in order to have the clinical requirements. In fact ion energy spectra have exponential trend, almost 100% energy spread and a wide angular divergence which is the biggest issue in the beam transport and, hence, in a wider use of this technology. In order to demonstrate the clinical applicability of laser-driven beams new collaboration between ELI-Beamlines project researchers from Prague (Cz) and a INFN-LNS group from Catania (I) has been already launched and scientists from different countries have already express their will in joining the project. This cooperation has been named ELIMED (MEDical application at ELIBeamlines) and will take place inside the ELI-Beamlines infrastructure located in Prague. This work describes the schedule of the ELIMED project and the design of the energy selector which will be realized at INFN-LNS. The device is an important part of the whole transport beam line which will be realised in order to make the ion beams suitable for medical applications.

  14. Preliminary timing measurements on a data acquisition chain for a SiPM-based detector for prostate imaging

    NASA Astrophysics Data System (ADS)

    Gabrielli, A.; Antonioli, P.; D'Antone, I.; Giorgi, F. M.; Lax, I.; Lolli, M.; De Leo, R.; De Robertis, G.; Foresta, M.; Loddo, F.; Ranieri, A.; Cosentino, G.; Finocchiaro, P.; Pappalardo, A. D.; Fonte, R.; Calvini, C.; Musico, P.; Ottonello, P.; Cusanno, F.; Garibaldi, F.; Gigli, T.; Giove, F.; Maraviglia, B.; Meddi, F.; Rivetti, A.

    2011-06-01

    Prostate cancer (PC) is one of the most common diseases in western countries and a leading cause of cancer death. A variety of treatment options are available but a precise disease characterization is needed: evaluation of cancer location, size, and extent and an indication of tumor aggressiveness. The current standard for diagnosing PC is transrectal ultrasound guided sextant biopsy. A novel multidisciplinary approach is required. Imaging may play a key role provided that dedicated prostate imagers and procedures are available: considerable improvements have been achieved in diagnosis with the Magnetic Resonance Imaging (MRI) and nuclear medicine (Positron Emission Tomography (PET) and Single Photon Emission Computed Tomography (SPECT)) techniques. Nevertheless, due to sub-optimal prostate imaging geometries with these generic large instruments preventing separation of the signal from surrounding organs, the sensitivity, spatial resolution and lesion contrast detected are lower compared to what can be potentially achievable with optimized dedicated prostate imagers and procedures. Fully exploiting the Time Of Flight (TOF) capability would allow not only to increase the Signal-to-Noise ratio (SNR) / Noise Equivalent Count Rate (NECR) but also to get rid of the huge background coming from neighboring organs i.e. the bladder. Recently a new research project was initiated by a large INFN collaboration, and a "TOF-PET and MRI for prostate cancer diagnosis and follow up experiment" (TOPEM) was financed as a 3-year experiment by the Italian "INFN Commissione Scientifica Nazionale V" with the goal of designing, building and testing in phantom tests an endorectal PET-TOF probe compatible with MRI.

  15. The EEE Project

    NASA Astrophysics Data System (ADS)

    Abbrescia, M.; An, S.; Antolini, R.; Badala, A.; Baldini Ferroli, R.; Bencivenni, G.; Blanco, F.; Bressan, E.; Chiavassa, A.; Chiri, C.; Cifarelli, L.; Cindolo, F.; Coccia, E.; de Pasquale, S.; di Giovanni, A.; d'Incecco, M.; Fabbri, F.L.; Frolov, V.; Garbini, M.; Gustavino, C.; Hatzifotiadou, D.; Imponente, G.; Kim, J.; La Rocca, P.; Librizzi, F.; Maggiora, A.; Menghetti, H.; Miozzi, S.; Moro, R.; Panareo, M.; Pappalardo, G.S.; Piragino, G.; Riggi, F.; Romano, F.; Sartorelli, G.; Sbarra, C.; Selvi, M.; Serci, S.; WIlliams, C.; Zichichi, A.; Zuyenski, R.

    The EEE (/Extreme Energy Event/) Project is an experiment for the study of very high-energy extensive air showers, actually starting in Italy. It is based on the detection of the shower muon component by means of a network of tracking detectors, installed in Italian High Schools. The Project, supported by the Ministero dell’Università e della Ricerca (MIUR), Istituto Nazionale di Fisica Nucleare (INFN), European Organization for Nuclear Research (CERN) and Museo Storico della Fisica e Centro Studi e Ricerche “E. Fermi” has been conceived by its leader Professor Antonino Zichichi. In its first phase the detector telescopes will be installed in 21 High Schools in 7 piloting cities all over Italy. The network will soon be heavily upgraded by increasing the number of High Schools and cities. The single tracking telescope is composed by 3 large (~ 2 m2 ) Multi-gap Resistive Plate Chambers (MRPC), realized with float glass electrodes. The use of particle detectors based on such MRPCs will allow to determine with a very high accuracy the direction of the axis of cosmic ray showers initiated by primaries of ultra-high energy, together with a high temporal resolution. The first MRPC telescope, installed in the Liceo Scientifico “B.Touschek” in Grottaferrata near the LNF-INFN site (nearby Rome), is successfully running. By the end of year 2007, the installation of the other telescopes will open the way for the first search of high-energy cosmic rays distant coincidences. In the future, serving many High Schools scattered all over the Italian territory, the EEE Project will also allow to investigate coincidences between multiple primaries producing distant showers. Here we present the experimental apparatus and its tasks.

  16. ILC Vertex Tracker R&D

    SciTech Connect

    Battaglia, Marco; Bussat, Jean-Marie; Contarato, Devis; Denes,Peter; Glesener, Lindsay; Greiner, Leo; Hooberman, Benjamin; Shuman,Derek; Tompkins, Lauren; Vu, Chinh; Bisello, Dario; Giubilato, Piero; Pantano, Devis; Costa, Marco; La Rosa, Alessandro; Bolla, Gino; Bortoletto, Daniela; Children, Isaac

    2007-10-01

    This document summarizes past achievements, current activities and future goals of the R&D program aimed at the design, prototyping and characterization of a full detector module, equipped with monolithic pixel sensors, matching the requirements for the Vertex Tracker at the ILC. We provide a plan of activities to obtain a demonstrator multi-layered vertex tracker equipped with sensors matching the ILC requirements and realistic lightweight ladders in FY11, under the assumption that ILC detector proto-collaborations will be choosing technologies and designs for the Vertex Tracker by that time. The R&D program discussed here started at LBNL in 2004, supported by a Laboratory Directed R&D (LDRD) grant and by funding allocated from the core budget of the LBNL Physics Division and from the Department of Physics at UC Berkeley. Subsequently additional funding has been awarded under the NSF-DOE LCRD program and also personnel have become available through collaborative research with other groups. The aim of the R&D program carried out by our collaboration is to provide a well-integrated, inclusive research effort starting from physics requirements for the ILC Vertex Tracker and addressing Si sensor design and characterization, engineered ladder design, module system issues, tracking and vertex performances and beam test validation. The broad scope of this program is made possible by important synergies with existing know-how and concurrent programs both at LBNL and at the other collaborating institutions. In particular, significant overlaps with LHC detector design, SLHC R&D as well as prototyping for the STAR upgrade have been exploited to optimize the cost per deliverable of our program. This activity is carried out as a collaborative effort together with Accelerator and Fusion Research, the Engineering and the Nuclear Science Divisions at LBNL, INFN and the Department of Physics in Padova, Italy, INFN and the Department of Physics in Torino, Italy and the Department

  17. A prototype Infrastructure for Cloud-based distributed services in High Availability over WAN

    NASA Astrophysics Data System (ADS)

    Bulfon, C.; Carlino, G.; De Salvo, A.; Doria, A.; Graziosi, C.; Pardi, S.; Sanchez, A.; Carboni, M.; Bolletta, P.; Puccio, L.; Capone, V.; Merola, L.

    2015-12-01

    In this work we present the architectural and performance studies concerning a prototype of a distributed Tier2 infrastructure for HEP, instantiated between the two Italian sites of INFN-Romal and INFN-Napoli. The network infrastructure is based on a Layer-2 geographical link, provided by the Italian NREN (GARR), directly connecting the two remote LANs of the named sites. By exploiting the possibilities offered by the new distributed file systems, a shared storage area with synchronous copy has been set up. The computing infrastructure, based on an OpenStack facility, is using a set of distributed Hypervisors installed in both sites. The main parameter to be taken into account when managing two remote sites with a single framework is the effect of the latency, due to the distance and the end-to-end service overhead. In order to understand the capabilities and limits of our setup, the impact of latency has been investigated by means of a set of stress tests, including data I/O throughput, metadata access performance evaluation and network occupancy, during the life cycle of a Virtual Machine. A set of resilience tests has also been performed, in order to verify the stability of the system on the event of hardware or software faults. The results of this work show that the reliability and robustness of the chosen architecture are effective enough to build a production system and to provide common services. This prototype can also be extended to multiple sites with small changes of the network topology, thus creating a National Network of Cloud-based distributed services, in HA over WAN.

  18. PREFACE Preface

    NASA Astrophysics Data System (ADS)

    Bleicher, Markus; Caines, Helen; Calderon de la Barca Sanchez, Manuel; de Falco, Alessandro; Fries, Rainer; Granier de Cassagnac, Raphael; Hippolyte, Boris; Mischke, Andre; Nardi, Marzia; Salgado, Carlos A.

    2011-01-01

    The 4th Workshop for Young Scientists on the Physics of Ultrarelativistic Nucleus-nucleus Collisions (Hot Quarks 2010) was held in La Londe-Les-Maures, France, from June 21-26, 2010. Following the traditions of the conference, this meeting gathered more than 70 participants in the first years of their scientific careers. The present issue contains the proceedings of this workshop. The articles published in this volume clearly show the presence of a dynamic new generation of physicists interested in the different aspects of high energy nuclear collisions. The newest results from RHIC at Brookhaven and SPS at CERN were presented, as well as the latest results from the proton-proton programme from the LHC at CERN, while waiting for the data of the lead-lead collisions only available some months after the meeting. Along with these experimental findings, the corresponding theoretical research was also extensively discussed as well as the new perspectives for future facilities like FAIR, EIC and LHeC. We wish to thank the sponsors of the Hot Quarks 2010 Conference, who supported the authors of this volume: IN2P3/CNRS (France), EMMI (Germany), Institut Pluridisciplinaire Hubert Curien (France), National Science Foundation (USA), CERN (Switzerland), Helmholtz International Center for FAIR (Germany), Xunta de Galicia (Spain) and the Journal of Physics G. Markus Bleicher (Frankfurt (HIC4FAIR), Germany)Helen Caines (Yale University, USA)Manuel Calderon de la Barca Sanchez (UC Davis, USA)Alessandro de Falco (Cagliari/INFN, Italy)Rainer Fries (Texas A & M University, USA) Raphael Granier de Cassagnac (Ecole Polytechnique, France)Boris Hippolyte (IPHC, Strasbourg, France)Andre Mischke (Utrecht University, The Netherlands)Marzia Nardi (Torino/INFN, Italy)Carlos A Salgado (Universidade de Santiago de Compostela, Galicia, Spain)

  19. Theoretical Virtual Observatory and Grid Web services: VisIVO and new capabilities

    NASA Astrophysics Data System (ADS)

    Costa, A.; Becciani, U.; Gheller, C.; Comparato, M.; Larsson, B.

    2007-08-01

    VisIVO is a tool for 3D visualization, it provides an effective and intuitive way of managing, visualizing and analysing the large amount of data produced by observations and numerical simulations. The software is specifically designed to deal with multidimensional data. Catalogues and numerical simulations represent the basic target of VisIVO. The package is written in C++. This poster describes VIsIVO's Grid Web Service (VWS) and its counterpart client side developed in VisIVO. VisIVO's Grid Web Service is developed as a part of the Italian Virtual Observatory, it allows to run applications in grid using the web service technology. The VWS has been designed to work within a Virtual Observatory environment so that the interface for the current application, shown in this poster, and the interfaces for other applications are described by a small and constant piece of WSDL code. Our first application is HOP: an algorithm for finding groups of particles based on the one developed and coded by Daniel Eisenstein & Piet Hut, Institute for Advanced Study, Princeton, NJ. We have developed VWS using Java AXIS libraries for the server side and C++ AXIS libraries for the client side. The access to the computational resources and storage areas is based on grid services in the INFN Production Grid and from this the VWS inherits asynchronous features, scheduling and matching algorithms. HOP was distributed as RPM Package and was installed in the Worker Node Elements of the INFN Production Grid. The idea is to use external tools through the grid avoiding the integration in our application. We can focus on the I/O management of the jobs and on the standardization of the access methods for the different analysis tools improving the scalability of our solution.

  20. The Science Training Program for Young Italian Physicists and Engineers at Fermilab

    SciTech Connect

    Barzi, Emanuela; Bellettini, Giorgio; Donati, Simone

    2015-03-12

    Since 1984 Fermilab has been hosting a two-month summer training program for selected undergraduate and graduate Italian students in physics and engineering. Building on the traditional close collaboration between the Italian National Institute of Nuclear Physics (INFN) and Fermilab, the program is supported by INFN, by the DOE and by the Scuola Superiore di Sant`Anna of Pisa (SSSA), and is run by the Cultural Association of Italians at Fermilab (CAIF). This year the University of Pisa has qualified it as a “University of Pisa Summer School”, and will grant successful students with European Supplementary Credits. Physics students join the Fermilab HEP research groups, while engineers join the Particle Physics, Accelerator, Technical, and Computing Divisions. Some students have also been sent to other U.S. laboratories and universities for special trainings. The programs cover topics of great interest for science and for social applications in general, like advanced computing, distributed data analysis, nanoelectronics, particle detectors for earth and space experiments, high precision mechanics, applied superconductivity. In the years, over 350 students have been trained and are now employed in the most diverse fields in Italy, Europe, and the U.S. In addition, the existing Laurea Program in Fermilab Technical Division was extended to the whole laboratory, with presently two students in Master’s thesis programs on neutrino physics and detectors in the Neutrino Division. And finally, a joint venture with the Italian Scientists and Scholars North-America Foundation (ISSNAF) provided this year 4 professional engineers free of charge for Fermilab. More details on all of the above can be found below.

  1. The MAGNEX spectrometer: Results and perspectives

    NASA Astrophysics Data System (ADS)

    Cappuzzello, F.; Agodi, C.; Carbone, D.; Cavallaro, M.

    2016-06-01

    This review discusses the main achievements and future perspectives of the MAGNEX spectrometer at the INFN-LNS laboratory in Catania (Italy). MAGNEX is a large-acceptance magnetic spectrometer for the detection of the ions emitted in nuclear collisions below Fermi energy. In the first part of the paper an overview of the MAGNEX features is presented. The successful application to the precise reconstruction of the momentum vector, to the identification of the ion masses and to the determination of the transport efficiency is demonstrated by in-beam tests. In the second part, an overview of the most relevant scientific achievements is given. Results from nuclear elastic and inelastic scattering as well as from transfer and charge-exchange reactions in a wide range of masses of the colliding systems and incident energies are shown. The role of MAGNEX in solving old and new puzzles in nuclear structure and direct reaction mechanisms is emphasized. One example is the recently observed signature of the long searched Giant Pairing Vibration. Finally, the new challenging opportunities to use MAGNEX for future experiments are briefly reported. In particular, the use of double charge-exchange reactions toward the determination of the nuclear matrix elements entering in the expression of the half-life of neutrinoless double beta decay is discussed. The new NUMEN project of INFN, aiming at these investigations, is introduced. The challenges connected to the major technical upgrade required by the project in order to investigate rare processes under high fluxes of detected heavy ions are outlined.

  2. Next-generation Lunar Laser Retroreflectors for Precision Tests of General Relativity

    NASA Astrophysics Data System (ADS)

    Ciocci, Emanuele; dell'Agnello, Simone; Delle Monache, Giovanni; Martini, Manuele; Contessa, Stefania; Porcelli, Luca; Tibuzzi, Mattia; Salvatori, Lorenzo; Patrizi, Giordano; Maiello, Mauro; Intaglietta, Nicola; Mondaini, Chiara; Currie, Douglas; Chandler, John; Bianco, Giuseppe; Murphy, Tom

    2016-04-01

    Since 1969, Lunar Laser Ranging (LLR) to the Apollo Cube Corner Retroreflectors (CCRs) has supplied almost all significant tests of General Relativity (GR). When first installed in the 1970s, the Apollo CCRs geometry contributed only a negligible fraction of the ranging error budget. Today, because of lunar librations, this contribution dominates the error budget, limiting the precision of the experimental tests of gravitational theories. The new MoonLIGHT-2 (Moon Laser Instrumentation for General relativity High-accuracy Tests) apparatus is a new-generation LLR payload developed by the SCF_Lab (http://www.lnf.infn.it/esperimenti/etrusco/) at INFN-LNF in collaboration with the Maryland University. With the unique design of a single large CCR unaffected by librations, MoonLIGHT-2 can increase up to a factor 100 the precision of the measurement of the lunar geodetic precession and other General Relativity (GR) tests respect to Apollo CCRs. MoonLIGHT-2 is approved to be launched with the Moon Express mission MEX-1 and will be deployed on the Moon surface in 2018. MoonLIGHT-2 is also proposed for the Roscosmos mission Luna-27. To validate/optimize MoonLIGHT-2 for MEX-1, the SCF_Lab is carrying out a unique experimental test called SCF-Test: the concurrent measurement of the optical Far Field Diffraction Pattern (FFDP) and the temperature distribution of the CCR under thermal conditions produced with a close-match solar simulator and simulated space environment. We perform test of GR with current LLR data and also different GR simulation of the expected improvement in GR test provided by MoonLIGHT-2, using the Planetary Ephemeris Program in collaboration with CfA. Our ultimate goal is to improve GR tests by a factor up to 100, and provide constraints on the new gravitational theories like non-miminally coupled gravity and spacetime torision.

  3. Development and simulation results of a sparsification and readout circuit for wide pixel matrices

    NASA Astrophysics Data System (ADS)

    Gabrielli, A.; Giorgi, F.; Morsani, F.; Villa, M.

    2011-06-01

    In future collider experiments, the increasing luminosity and centre of mass energy are rising challenging problems in the design of new inner tracking systems. In this context we develop high-efficiency readout architectures for large binary pixel matrices that are meant to cope with the high-stressing conditions foreseen in the innermost layers of a tracker [The SuperB Conceptual Design Report, INFN/AE-07/02, SLAC-R-856, LAL 07-15, Available online at: http://www.pi.infn.it/SuperB]. We model and design digital readout circuits to be integrated on VLSI ASICs. These architectures can be realized with different technology processes and sensors: they can be implemented on the same silicon sensor substrate of a CMOS MAPS devices (Monolithic Active Pixel Sensor), on the CMOS tier of a hybrid pixel sensor or in a 3D chip where the digital layer is stacked on the sensor and the analog layers [V. Re et al., Nuc. Instr. and Meth. in Phys. Res. A, doi:10.1016/j.nima.2010.05.039]. In the presented work, we consider a data-push architecture designed for a sensor matrix of an area of about 1.3 cm 2 with a pitch of 50 microns. The readout circuit tries to take great advantage of the high density of in-pixel digital logic allowed by vertical integration. We aim at sustaining a rate density of 100 Mtrack ṡ s -1 ṡ cm -2 with a temporal resolution below 1 μs. We show how this architecture can cope with these stressing conditions presenting the results of Monte Carlo simulations.

  4. High density plasmas and new diagnostics: An overview (invited).

    PubMed

    Celona, L; Gammino, S; Mascali, D

    2016-02-01

    One of the limiting factors for the full understanding of Electron Cyclotron Resonance Ion Sources (ECRISs) fundamental mechanisms consists of few types of diagnostic tools so far available for such compact machines. Microwave-to-plasma coupling optimisation, new methods of density overboost provided by plasma wave generation, and magnetostatic field tailoring for generating a proper electron energy distribution function, suitable for optimal ion beams formation, require diagnostic tools spanning across the entire electromagnetic spectrum from microwave interferometry to X-ray spectroscopy; these methods are going to be implemented including high resolution and spatially resolved X-ray spectroscopy made by quasi-optical methods (pin-hole cameras). The ion confinement optimisation also requires a complete control of cold electrons displacement, which can be performed by optical emission spectroscopy. Several diagnostic tools have been recently developed at INFN-LNS, including "volume-integrated" X-ray spectroscopy in low energy domain (2-30 keV, by using silicon drift detectors) or high energy regime (>30 keV, by using high purity germanium detectors). For the direct detection of the spatially resolved spectral distribution of X-rays produced by the electronic motion, a "pin-hole camera" has been developed also taking profit from previous experiences in the ECRIS field. The paper will give an overview of INFN-LNS strategy in terms of new microwave-to-plasma coupling schemes and advanced diagnostics supporting the design of new ion sources and for optimizing the performances of the existing ones, with the goal of a microwave-absorption oriented design of future machines. PMID:26931960

  5. High density plasmas and new diagnostics: An overview (invited)

    NASA Astrophysics Data System (ADS)

    Celona, L.; Gammino, S.; Mascali, D.

    2016-02-01

    One of the limiting factors for the full understanding of Electron Cyclotron Resonance Ion Sources (ECRISs) fundamental mechanisms consists of few types of diagnostic tools so far available for such compact machines. Microwave-to-plasma coupling optimisation, new methods of density overboost provided by plasma wave generation, and magnetostatic field tailoring for generating a proper electron energy distribution function, suitable for optimal ion beams formation, require diagnostic tools spanning across the entire electromagnetic spectrum from microwave interferometry to X-ray spectroscopy; these methods are going to be implemented including high resolution and spatially resolved X-ray spectroscopy made by quasi-optical methods (pin-hole cameras). The ion confinement optimisation also requires a complete control of cold electrons displacement, which can be performed by optical emission spectroscopy. Several diagnostic tools have been recently developed at INFN-LNS, including "volume-integrated" X-ray spectroscopy in low energy domain (2-30 keV, by using silicon drift detectors) or high energy regime (>30 keV, by using high purity germanium detectors). For the direct detection of the spatially resolved spectral distribution of X-rays produced by the electronic motion, a "pin-hole camera" has been developed also taking profit from previous experiences in the ECRIS field. The paper will give an overview of INFN-LNS strategy in terms of new microwave-to-plasma coupling schemes and advanced diagnostics supporting the design of new ion sources and for optimizing the performances of the existing ones, with the goal of a microwave-absorption oriented design of future machines.

  6. List of Participants

    NASA Astrophysics Data System (ADS)

    2011-09-01

    AbeTakashiUniversity of Tokyotabe@nt.phys.s.u-tokyo.ac.jp AmusiaMironRacah Institute of Physics, Jerusalemamusia@vms.huji.ac.il BaldoMarcelloINFN Cataniabaldo@ct.infn.it BansalManiePanjab University, Chandigarhbansalmanni@gmail.com BarrancoFranciscoUniversity of Sevillebarranco@us.es BertschGeorgeUniversity of Washington, Seattlebertsch@u.washington.edu BhagwatAmeeyaCBS Mumbaiameeya@kth.se BorderieBernardIPN Orsayborderie@ipno.in2p3.fr CarbonellJaumeLPSC Grenoblejaume.carbonell@lpsc.in2p3.fr CarlsonJoeLos Alamos National Laboratorycarlson@lanl.gov ColòGianlucaINFN - Università degli Studi di Milanocolo@mi.infn.it DanielewiczPawelNSCL, Michigan State Universitydanielewicz@nscl.msu.edu DescouvemontPierreUniversité Libre de Bruxellespdesc@ulb.ac.be Dohet-EralyJérémyUniversité Libre de Bruxellesjdoheter@ulb.ac.be DraayerJerryLouisiana State Universitydraayer@lsu.edu DufourMarianneIPHC, Université de Strasbourgmarianne.dufour@ires.in2p3.fr DuguetThomasCEA Saclaythomas.duguet@cea.fr DukelskyJorgeCSIC Madriddukelsky@iem.cfmac.csic.es EbranJean-PaulCEA-DAM, Arpajonebran@ipno.in2p3.fr FreerMartinUniversity of Birminghamm.freer@bham.ac.uk FujiiShinichiroUniversity of Tokyosfujii@cns.s.u-tokyo.ac.jp FunakiYasuroRIKEN Nishina Center, Wakofunaki@riken.jp GrassoMarcellaIPN Orsaygrasso@ipno.in2p3.fr HaginoKouichiTohoku Universityhagino@nucl.phys.tohoku.ac.jp HansenHubertUniversité Claude Bernard Lyon 1hansen@ipnl.in2p3.fr HolzmannMarkusLPMMC Grenoblemarkus@lptl.jussieu.fr HoriuchiHisashiRCNP, Osaka Universityhoriuchi@rcnp.osaka-u.ac.jp HoriuchiWataruGSI Darmstadtw.horiuchi@gsi.de HupinGuillaumeGANIL, Caenhupin@ganil.fr JinMengHuazhong Normal University, Wuhanjinm@iopp.ccnu.edu.cn KamimuraMasayasuRIKEN Nishina Center, Wakomkamimura@riken.jp Kanada-En'yoYoshikoKyoto Universityyenyo@ruby.scphys.kyoto-u.ac.jp KatoKiyoshiHokkaido University, Sapporokato@nucl.sci.hokudai.ac.jp KawabataTakahiroKyoto Universitykawabata@scphys.kyoto-u.ac.jp KhanEliasIPN Orsaykhan@ipno.in2p3.fr

  7. PREFACE: International Conference on Topics in Astroparticle and Underground Physics (TAUP 2011)

    NASA Astrophysics Data System (ADS)

    Oberauer, Lothar; Raffelt, Georg; Wagner, Robert

    2012-07-01

    The 12th edition of the International Conference on Topics in Astroparticle and Underground Physics (TAUP 2011) was held 5-9 September 2011 in Munich (and for the first time in Germany). It was organized by the Max Planck Institute for Physics (MPP), the Technical University Munich (TUM) and the Cluster of Excellence 'Origin and Structure of the Universe'. The conference was held in the 'Künstlerhaus', a traditional downtown location for artistic festivities. The meeting attracted 317 participants (61 of which were women) from 29 countries, see figure below. The topics covered by the meeting were Cosmology and particle physics, Dark matter and its detection, Neutrino physics and astrophysics, Gravitational waves and High-energy astrophysics and cosmic rays, and the various interfaces between these areas. The scientific sessions consisted of five mornings of plenary talks, four afternoons of parallel sessions, and an evening poster session. The co-founder of the conference series, Alessandro Bottino, has decided to retire from the position of chairman of the TAUP Steering Committee after the completion of TAUP 2011. On behalf of all followers of this series, we thank him for having started these inspiring events and his many years of dedicated service. We thank all speakers, conveners and participants as well as the members of the organizing, steering and international advisory committee for making this a successful and memorable meeting. Lothar Oberauer, Georg Raffelt, Robert Wagner Proceedings editors Figure Committees International Advisory Committee G AntonUniversity of Erlangen E AprileColumbia University M Baldo-CeolinUniversity of Padova R BattistonUniversity of Perugia & INFN L BergströmUniversity Stockholm R BernabeiUniversity of Rome 'Tor Vergata' A BettiniLSC Canfranc P BinetruyAPC Paris J BlümerKarlsruhe Institute of Technology B CabreraStanford University A CaldwellMax Planck Institute for Physics M ChenQueens University E CocciaUniversity of Rome

  8. Modulation of Cell Metabolic Pathways and Oxidative Stress Signaling Contribute to Acquired Melphalan Resistance in Multiple Myeloma Cells

    PubMed Central

    Zub, Kamila Anna; de Sousa, Mirta Mittelstedt Leal; Sarno, Antonio; Sharma, Animesh; Demirovic, Aida; Rao, Shalini; Young, Clifford; Aas, Per Arne; Ericsson, Ida; Sundan, Anders; Jensen, Ole Nørregaard; Slupphaug, Geir

    2015-01-01

    Alkylating agents are widely used chemotherapeutics in the treatment of many cancers, including leukemia, lymphoma, multiple myeloma, sarcoma, lung, breast and ovarian cancer. Melphalan is the most commonly used chemotherapeutic agent against multiple myeloma. However, despite a 70–80% initial response rate, virtually all patients eventually relapse due to the emergence of drug-resistant tumour cells. By using global proteomic and transcriptomic profiling on melphalan sensitive and resistant RPMI8226 cell lines followed by functional assays, we discovered changes in cellular processes and pathways not previously associated with melphalan resistance in multiple myeloma cells, including a metabolic switch conforming to the Warburg effect (aerobic glycolysis), and an elevated oxidative stress response mediated by VEGF/IL8-signaling. In addition, up-regulated aldo-keto reductase levels of the AKR1C family involved in prostaglandin synthesis contribute to the resistant phenotype. Finally, selected metabolic and oxidative stress response enzymes were targeted by inhibitors, several of which displayed a selective cytotoxicity against the melphalan-resistant cells and should be further explored to elucidate their potential to overcome melphalan resistance. PMID:25769101

  9. Aldose reductase C-106T polymorphism is associated with the risk of essential hypertension.

    PubMed

    Wang, Yaqin; Yu, Min; Mo, Long; Li, Zhenyu; Wang, Junjie; Zhou, Hong-Hao; Ouyang, Dong-Sheng

    2016-10-10

    Aldose Reductase (AR), encoded by AKR1B1, is a member of NADPH-dependent aldo-keto reductase superfamily. The C-106T polymorphism of AKR1B1 is closely related to the diabetic complications. Our previous studies have indicated that the expression of AR was increased in spontaneously hypertensive rats, suggesting the effect of AR in hypertension. Here we investigated whether AKR1B1 C-106T polymorphism was associated with essential hypertension (EH). AKR1B1 C-106T polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the direct sequencing methods. 383 healthy subjects and 383 essential hypertensive patients were recruited in this study. The polymorphism of AKR1B1 C-106T in EH and normal tensive (NT) groups was in agreement with Hardy-Weinberg equilibrium. -106T allele of AKR1B1 C-106T variants was more frequent in EH patients compared with normal tensive subjects, indicating that -106T allele was a risk factor of EH (OR=1.841, 95%CI=1.366-2.481). In male patients, C-106T polymorphism was associated significantly with decreased serum high density lipoprotein cholesterol and higher systolic blood pressure levels. Our results suggest that -106T allele of AKR1B1 C-106T polymorphism may be associated with increased risk for EH in Chinese Han population. PMID:27343777

  10. Oxidative Stress and Carbonyl Lesions in Ulcerative Colitis and Associated Colorectal Cancer

    PubMed Central

    Wang, Zhiqi; Li, Sai; Cao, Yu; Tian, Xuefei; Zeng, Rong; Liao, Duan-Fang; Cao, Deliang

    2016-01-01

    Oxidative stress has long been known as a pathogenic factor of ulcerative colitis (UC) and colitis-associated colorectal cancer (CAC), but the effects of secondary carbonyl lesions receive less emphasis. In inflammatory conditions, reactive oxygen species (ROS), such as superoxide anion free radical (O2∙−), hydrogen peroxide (H2O2), and hydroxyl radical (HO∙), are produced at high levels and accumulated to cause oxidative stress (OS). In oxidative status, accumulated ROS can cause protein dysfunction and DNA damage, leading to gene mutations and cell death. Accumulated ROS could also act as chemical messengers to activate signaling pathways, such as NF-κB and p38 MAPK, to affect cell proliferation, differentiation, and apoptosis. More importantly, electrophilic carbonyl compounds produced by lipid peroxidation may function as secondary pathogenic factors, causing further protein and membrane lesions. This may in turn exaggerate oxidative stress, forming a vicious cycle. Electrophilic carbonyls could also cause DNA mutations and breaks, driving malignant progression of UC. The secondary lesions caused by carbonyl compounds may be exceptionally important in the case of host carbonyl defensive system deficit, such as aldo-keto reductase 1B10 deficiency. This review article updates the current understanding of oxidative stress and carbonyl lesions in the development and progression of UC and CAC. PMID:26823956

  11. Involvement of an octose ketoreductase and two acyltransferases in the biosynthesis of paulomycins

    NASA Astrophysics Data System (ADS)

    Li, Jine; Wang, Min; Ding, Yong; Tang, Yue; Zhang, Zhiguo; Chen, Yihua

    2016-02-01

    C-4 hydroxyethyl branched octoses have been observed in polysaccharides of several genera of gram negative bacteria and in various antibiotics produced by gram positive bacteria. The C-4 hydroxyethyl branch was proposed to be converted from C-4 acetyl branch by an uncharacterized ketoreduction step. Paulomycins (PAUs) are glycosylated antibiotics with potent inhibitory activity against gram positive bacteria and are structurally defined by its unique C-4‧ hydroxyethyl branched paulomycose moiety. A novel aldo-keto-reductase, Pau7 was characterized as the enzyme catalyzing the stereospecific ketoreduction of 7‧-keto of PAU E (1) to give the C-4‧ hydroxyethyl branched paulomycose moiety of PAU F (2). An acyltransferase Pau6 further decorates the C-4‧ hydroxyethyl branch of paulomycose moiety of 2 by attaching various fatty acyl chains to 7‧-OH to generate diverse PAUs. In addition, another acyltransferase Pau24 was proposed to be responsible for the 13-O-acetylation of PAUs.

  12. DNA from uncultured organisms as a source of 2,5-diketo-L-gluconic acid reductases.

    SciTech Connect

    Eschenfeldt, W. H.; Stols, L.; Rosenbaum, H.; Khambatta, Z. S.; Quaite, E. R.; Wu, S.; Kilgore, D. C.; Trent, J. D.; Donnelly, M. I.; Genencor International; Eastman Chemical Company

    2001-09-01

    Total DNA of a population of uncultured organisms was extracted from soil samples, and by using PCR methods, the genes encoding two different 2,5-diketo-D-gluconic acid reductases (DKGRs) were recovered. Degenerate PCR primers based on published sequence information gave internal gene fragments homologous to known DKGRs. Nested primers specific for the internal fragments were combined with random primers to amplify flanking gene fragments from the environmental DNA, and two hypothetical full-length genes were predicted from the combined sequences. Based on these predictions, specific primers were used to amplify the two complete genes in single PCRs. These genes were cloned and expressed in Escherichia coli. The purified gene products catalyzed the reduction of 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid. Compared to previously described DKGRs isolated from Corynebacterium spp., these environmental reductases possessed some valuable properties. Both exhibited greater than 20-fold-higher k{sub cat}/K{sub m} values than those previously determined, primarily as a result of better binding of substrate. The K{sub m} values for the two new reductases were 57 and 67 {mu}M, versus 2 and 13 mM for the Corynebacterium enzymes. Both environmental DKGRs accepted NADH as well as NADPH as a cosubstrate; other DKGRs and most related aldo-keto reductases use only NADPH. In addition, one of the new reductases was more thermostable than known DKGRs.

  13. Cardiolipin as key lipid of mitochondria in health and disease. 2nd Edition, Florence, Italy, September 30-October 1, 2015.

    PubMed

    Corcelli, Angela; Schlame, Michael

    2016-06-01

    The second edition of the workshop dedicated to cardiolipin, the signature lipid of mitochondria, was held as a satellite meeting of the 13th Euro Fed Lipid international congress in Florence, Italy, at the end of September 2015. During the workshop various aspects of basic cardiolipin functions in biomembranes of prokaryotes and animal cells were discussed, highlighting connections between cardiolipin research and human physiology in particular. Alteration of the cardiolipin species pattern and a parallel increase of monolysocardiolipin is the hallmark of Barth syndrome, an X linked genetic disease. Furthermore literature reports suggest the involvement of cardiolipin in other pathologies associated with an imbalance in bioenergetic functions, such as diabetes. The Cardiolipin Workshop was a low budget meeting sponsored by the University of Bari Aldo Moro and the Barth Syndrome Foundation. The organizers are grateful to the invited speakers, poster presenters and chairpersons as they supported the meeting by sustaining their travel and lodging expenses. Before the starting of the scientific sessions, the families of Italian boys affected by Barth syndrome (recently affiliated with the Barth Syndrome Foundation, USA) could meet scientists, biologists and pediatricians involved in research, diagnosis and cure of the disease. The family meeting was chaired by Daniela Toniolo, who discovered the tafazzin gene in 1997. PMID:27132118

  14. Molecular cloning of mannose-6-phosphate reductase and its developmental expression in celery.

    PubMed Central

    Everard, J D; Cantini, C; Grumet, R; Plummer, J; Loescher, W H

    1997-01-01

    Compared with other primary photosynthetic products (e.g. sucrose and starch), little is known about sugar alcohol metabolism, its regulation, and the manner in which it is integrated with other pathways. Mannose-6-phosphate reductase (M6PR) is a key enzyme that is involved in mannitol biosynthesis in celery (Apium graveolens L.). The M6PR gene was cloned from a leaf cDNA library, and clonal authenticity was established by assays of M6PR activity, western blots, and comparisons of the deduced amino acid sequence with a celery M6PR tryptic digestion product. Recombinant M6PR, purified from Escherichia coli, had specific activity, molecular mass, and kinetic characteristics indistinguishable from those of authentic celery M6PR. Sequence analyses showed M6PR to be a member of the aldo-keto reductase superfamily, which includes both animal and plant enzymes. The greatest sequence similarity was with aldose-6-phosphate reductase (EC 1.1.1.200), a key enzyme in sorbitol synthesis in Rosaceae. Developmental studies showed M6PR to be limited to green tissues and to be under tight transcriptional regulation during leaf initiation, expansion, and maturation. These data confirmed a close relationship between the development of photosynthetic capacity, mannitol synthesis, and M6PR activity. PMID:9112783

  15. Comparison of Instantaneous Frequency Scaling from Rain Attenuation and Optical Disdrometer Measurements at K/Q bands

    NASA Technical Reports Server (NTRS)

    Nessel, James; Zemba, Michael; Luini, Lorenzo; Riva, Carlo

    2015-01-01

    Rain attenuation is strongly dependent on the rain rate, but also on the rain drop size distribution (DSD). Typically, models utilize an average drop size distribution, such as those developed by Laws and Parsons, or Marshall and Palmer. However, individual rain events may possess drop size distributions which could be significantly different from the average and will impact, for example, fade mitigation techniques which utilize channel performance estimates from a signal at a different frequency. Therefore, a good understanding of the characteristics and variability of the raindrop size distribution is extremely important in predicting rain attenuation and instantaneous frequency scaling parameters on an event-toevent basis. Since June 2014, NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have measured the attenuation due to rain in Milan, Italy, on the 20/40 GHz beacon signal broadcast from the Alphasat TDP#5 Aldo Paraboni Q/V-band Payload. Concomitant with these measurements are the measurements of drop size distribution and rain rate utilizing a Thies Clima laser precipitation monitor (disdrometer). In this paper, we discuss the comparison of the predicted rain attenuation at 20 and 40 GHz derived from the drop size distribution data with the measured rain attenuation. The results are compared on statistical and real-time bases. We will investigate the performance of the rain attenuation model, instantaneous frequency scaling, and the distribution of the scaling factor. Further, seasonal rain characteristics will be analysed.

  16. Structure and Catalytic Mechanism of Human Steroid 5-Reductase (AKR1D1)

    SciTech Connect

    Costanzo, L.; Drury, J; Christianson, D; Penning, T

    2009-01-01

    Human steroid 5{beta}-reductase (aldo-keto reductase (AKR) 1D1) catalyzes reduction of {Delta}{sup 4}-ene double bonds in steroid hormones and bile acid precursors. We have reported the structures of an AKR1D1-NADP{sup +} binary complex, and AKR1D1-NADP{sup +}-cortisone, AKR1D1-NADP{sup +}-progesterone and AKR1D1-NADP{sup +}-testosterone ternary complexes at high resolutions. Recently, structures of AKR1D1-NADP{sup +}-5{beta}-dihydroprogesterone complexes showed that the product is bound unproductively. Two quite different mechanisms of steroid double bond reduction have since been proposed. However, site-directed mutagenesis supports only one mechanism. In this mechanism, the 4-pro-R hydride is transferred from the re-face of the nicotinamide ring to C5 of the steroid substrate. E120, a unique substitution in the AKR catalytic tetrad, permits a deeper penetration of the steroid substrate into the active site to promote optimal reactant positioning. It participates with Y58 to create a 'superacidic' oxyanion hole for polarization of the C3 ketone. A role for K87 in the proton relay proposed using the AKR1D1-NADP{sup +}-5{beta}-dihydroprogesterone structure is not supported.

  17. Embodiment and enculturation: the future of architectural design.

    PubMed

    Mallgrave, Harry F

    2015-01-01

    A half-century ago the Dutch architect Aldo van Eyck encouraged designers to think about "space and time" not as abstractions in themselves but rather as cultural events better approached through the medium of "place and occasion." Van Eyck made this point on the basis of his own travels and through his extensive readings in cultural anthropology, and his prescience is only now acquiring the credibility that it deserves through the work of a multitude of interdisciplinary researchers. Phenomenologists argue that we are embodied organisms-acting-within-environments, and these inhabiting abodes are constructed of both material and cultural dimensions. We are thus preeminently social in our range of self-consciousness, and intensely ceremonial in every facet of our being. Evolutionary psychologists and anthropologists are currently locating the origin and development of our most basic social behaviors far in our pre-human past; neuroscientists are today modeling our social circuits in the deepest reaches of our brains. Architecture would gain much from an updated cultural theory grounded in these new models of human existence. PMID:26441773

  18. Induction of a Unique Isoform of the NCOA7 Oxidation Resistance Gene by Interferon β-1b

    PubMed Central

    Yu, Lijian; Croze, Ed; Yamaguchi, Ken D.; Tran, Tiffany; Reder, Anthony T.; Litvak, Vladimir

    2015-01-01

    We demonstrate that interferon (IFN)-β-1b induces an alternative-start transcript containing the C-terminal TLDc domain of nuclear receptor coactivator protein 7 (NCOA7), a member of the OXR family of oxidation resistance proteins. IFN-β-1b induces NCOA7-AS (alternative start) expression in peripheral blood mononuclear cells (PBMCs) obtained from healthy individuals and multiple sclerosis patients and human fetal brain cells, astrocytoma, neuroblastoma, and fibrosarcoma cells. NCOA7-AS is a previously undocumented IFN-β-inducible gene that contains only the last 5 exons of full-length NCOA7 plus a unique first exon (exon 10a) that is not found in longer forms of NCOA7. This exon encodes a domain closely related to an important class of bacterial aldo-keto oxido-reductase proteins that play a critical role in regulating redox activity. We demonstrate that NCOA7-AS is induced by IFN and LPS, but not by oxidative stress and exhibits, independently, oxidation resistance activity. We further demonstrate that induction of NCOA7-AS by IFN is dependent on IFN-receptor activation, the Janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling pathway, and a canonical IFN-stimulated response element regulatory sequence upstream of exon 10a. We describe a new role for IFN-βs involving a mechanism of action that leads to an increase in resistance to inflammation-mediated oxidative stress. PMID:25330068

  19. Induction of a unique isoform of the NCOA7 oxidation resistance gene by interferon β-1b.

    PubMed

    Yu, Lijian; Croze, Ed; Yamaguchi, Ken D; Tran, Tiffany; Reder, Anthony T; Litvak, Vladimir; Volkert, Michael R

    2015-03-01

    We demonstrate that interferon (IFN)-β-1b induces an alternative-start transcript containing the C-terminal TLDc domain of nuclear receptor coactivator protein 7 (NCOA7), a member of the OXR family of oxidation resistance proteins. IFN-β-1b induces NCOA7-AS (alternative start) expression in peripheral blood mononuclear cells (PBMCs) obtained from healthy individuals and multiple sclerosis patients and human fetal brain cells, astrocytoma, neuroblastoma, and fibrosarcoma cells. NCOA7-AS is a previously undocumented IFN-β-inducible gene that contains only the last 5 exons of full-length NCOA7 plus a unique first exon (exon 10a) that is not found in longer forms of NCOA7. This exon encodes a domain closely related to an important class of bacterial aldo-keto oxido-reductase proteins that play a critical role in regulating redox activity. We demonstrate that NCOA7-AS is induced by IFN and LPS, but not by oxidative stress and exhibits, independently, oxidation resistance activity. We further demonstrate that induction of NCOA7-AS by IFN is dependent on IFN-receptor activation, the Janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling pathway, and a canonical IFN-stimulated response element regulatory sequence upstream of exon 10a. We describe a new role for IFN-βs involving a mechanism of action that leads to an increase in resistance to inflammation-mediated oxidative stress. PMID:25330068

  20. De Novo Sequencing and Analysis of Lemongrass Transcriptome Provide First Insights into the Essential Oil Biosynthesis of Aromatic Grasses.

    PubMed

    Meena, Seema; Kumar, Sarma R; Venkata Rao, D K; Dwivedi, Varun; Shilpashree, H B; Rastogi, Shubhra; Shasany, Ajit K; Nagegowda, Dinesh A

    2016-01-01

    Aromatic grasses of the genus Cymbopogon (Poaceae family) represent unique group of plants that produce diverse composition of monoterpene rich essential oils, which have great value in flavor, fragrance, cosmetic, and aromatherapy industries. Despite the commercial importance of these natural aromatic oils, their biosynthesis at the molecular level remains unexplored. As the first step toward understanding the essential oil biosynthesis, we performed de novo transcriptome assembly and analysis of C. flexuosus (lemongrass) by employing Illumina sequencing. Mining of transcriptome data and subsequent phylogenetic analysis led to identification of terpene synthases, pyrophosphatases, alcohol dehydrogenases, aldo-keto reductases, carotenoid cleavage dioxygenases, alcohol acetyltransferases, and aldehyde dehydrogenases, which are potentially involved in essential oil biosynthesis. Comparative essential oil profiling and mRNA expression analysis in three Cymbopogon species (C. flexuosus, aldehyde type; C. martinii, alcohol type; and C. winterianus, intermediate type) with varying essential oil composition indicated the involvement of identified candidate genes in the formation of alcohols, aldehydes, and acetates. Molecular modeling and docking further supported the role of identified protein sequences in aroma formation in Cymbopogon. Also, simple sequence repeats were found in the transcriptome with many linked to terpene pathway genes including the genes potentially involved in aroma biosynthesis. This work provides the first insights into the essential oil biosynthesis of aromatic grasses, and the identified candidate genes and markers can be a great resource for biotechnological and molecular breeding approaches to modulate the essential oil composition. PMID:27516768

  1. Detoxifying Enzymes at the Cross-Roads of Inflammation, Oxidative Stress, and Drug Hypersensitivity: Role of Glutathione Transferase P1-1 and Aldose Reductase

    PubMed Central

    Sánchez-Gómez, Francisco J.; Díez-Dacal, Beatriz; García-Martín, Elena; Agúndez, José A. G.; Pajares, María A.; Pérez-Sala, Dolores

    2016-01-01

    Phase I and II enzymes are involved in the metabolism of endogenous reactive compounds as well as xenobiotics, including toxicants and drugs. Genotyping studies have established several drug metabolizing enzymes as markers for risk of drug hypersensitivity. However, other candidates are emerging that are involved in drug metabolism but also in the generation of danger or costimulatory signals. Enzymes such as aldo-keto reductases (AKR) and glutathione transferases (GST) metabolize prostaglandins and reactive aldehydes with proinflammatory activity, as well as drugs and/or their reactive metabolites. In addition, their metabolic activity can have important consequences for the cellular redox status, and impacts the inflammatory response as well as the balance of inflammatory mediators, which can modulate epigenetic factors and cooperate or interfere with drug-adduct formation. These enzymes are, in turn, targets for covalent modification and regulation by oxidative stress, inflammatory mediators, and drugs. Therefore, they constitute a platform for a complex set of interactions involving drug metabolism, protein haptenation, modulation of the inflammatory response, and/or generation of danger signals with implications in drug hypersensitivity reactions. Moreover, increasing evidence supports their involvement in allergic processes. Here, we will focus on GSTP1-1 and aldose reductase (AKR1B1) and provide a perspective for their involvement in drug hypersensitivity. PMID:27540362

  2. Direct analysis of highly oxidised organic aerosol constituents by on-line ion trap mass spectrometry in the negative-ion mode.

    PubMed

    Warscheid, Bettina; Hoffmann, Thorsten

    2002-01-01

    On-line ion trap mass spectrometry (ITMS) enables the characterisation of constituents of biogenic secondary organic aerosols in complex organic reaction mixtures. This real-time analysis is achieved by directly introducing the airborne particles into the ion source of the mass spectrometer. Negative-ion chemical ionisation at atmospheric pressure (APCI(-)) was used as the ionisation method of choice. The aerosols were generated from the gas-phase ozonolysis of two C10H16-terpenes (alpha-pinene and limonene), and investigated by performing on-line APCI(-)-ITMS(n). Highly oxidised compounds were tentatively identified as important particle-phase products. Based on recent investigations of low-energy collision-induced dissociation pathways of a wide range of deprotonated multifunctional carboxylic acid species derived from monoterpene precursors (Warscheid B, Hoffmann T. Rapid Commun. Mass Spectrom. 2001; 15: 2259), the formation of structurally different C10H16O5 and C10H16O6 species, such as acidic esters from alpha-pinene and aldo-hydroxycarboxylic acids from limonene, is proposed. PMID:11870886

  3. DIFFERENTIAL PROTEOME ANALYSIS OF THE MALE AND FEMALE ANTENNAE FROM Holotrichia parallela.

    PubMed

    Zhang, Ju-Hong; Wang, Shang; Yang, Shuang; Yi, Jiankun; Liu, Yan; Xi, Jing-Hui

    2016-08-01

    To understand the olfactory mechanisms of Holotrichia parallela antennae in detecting volatile compounds in the environment, protein profiles of H. parallela antennae were analyzed using two-dimensional electrophoresis followed by mass spectrometry and bioinformatics analyses. Approximately 1,100 protein spots in silver staining gel were detected. Quantitative image analysis revealed that in total 47 protein spots showed significant changes in different genders of adult antennae. Thirty-five differentially expressed proteins were identified by Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF) tandem mass spectrometer, among which 65.7% are involved in carbohydrate and energy metabolism, antioxidant system, transport, and amino acid/nucleotide metabolism. Some proteins identified here have not been reported previously in insect antennae. Identified male-biased proteins included odorant-binding protein 4, pheromone-binding protein-related protein 2, odorant-binding protein 14, prophenoloxidase-I, acyl-CoA dehydrogenase, aldo-keto reductase-like, carbamoyl phosphate synthetase, etc. whereas some proteins are female biased, such as antennae-rich cytochrome P450, aldehyde dehydrogenase, and putative glutamine synthetase. Alterations in the levels of some proteins were further confirmed by real time polymerase chain reaction (RT-PCR). The proteomic resources displayed here are valuable for the discovery of proteins from H. parallela antennae. PMID:27396371

  4. De Novo Sequencing and Analysis of Lemongrass Transcriptome Provide First Insights into the Essential Oil Biosynthesis of Aromatic Grasses

    PubMed Central

    Meena, Seema; Kumar, Sarma R.; Venkata Rao, D. K.; Dwivedi, Varun; Shilpashree, H. B.; Rastogi, Shubhra; Shasany, Ajit K.; Nagegowda, Dinesh A.

    2016-01-01

    Aromatic grasses of the genus Cymbopogon (Poaceae family) represent unique group of plants that produce diverse composition of monoterpene rich essential oils, which have great value in flavor, fragrance, cosmetic, and aromatherapy industries. Despite the commercial importance of these natural aromatic oils, their biosynthesis at the molecular level remains unexplored. As the first step toward understanding the essential oil biosynthesis, we performed de novo transcriptome assembly and analysis of C. flexuosus (lemongrass) by employing Illumina sequencing. Mining of transcriptome data and subsequent phylogenetic analysis led to identification of terpene synthases, pyrophosphatases, alcohol dehydrogenases, aldo-keto reductases, carotenoid cleavage dioxygenases, alcohol acetyltransferases, and aldehyde dehydrogenases, which are potentially involved in essential oil biosynthesis. Comparative essential oil profiling and mRNA expression analysis in three Cymbopogon species (C. flexuosus, aldehyde type; C. martinii, alcohol type; and C. winterianus, intermediate type) with varying essential oil composition indicated the involvement of identified candidate genes in the formation of alcohols, aldehydes, and acetates. Molecular modeling and docking further supported the role of identified protein sequences in aroma formation in Cymbopogon. Also, simple sequence repeats were found in the transcriptome with many linked to terpene pathway genes including the genes potentially involved in aroma biosynthesis. This work provides the first insights into the essential oil biosynthesis of aromatic grasses, and the identified candidate genes and markers can be a great resource for biotechnological and molecular breeding approaches to modulate the essential oil composition. PMID:27516768

  5. GC/IR computer-aided identification of anaerobic bacteria

    NASA Astrophysics Data System (ADS)

    Ye, Hunian; Zhang, Feng S.; Yang, Hua; Li, Zhu; Ye, Song

    1993-09-01

    A new method was developed to identify anaerobic bacteria by using pattern recognition. The method is depended on GC / JR data. The system is intended for use as a precise rapid and reproduceable aid in the identification of unknown isolates. Key Words: Anaerobic bacteria Pattern recognition Computeraided identification GC / JR 1 . TNTRODUCTTON A major problem in the field of anaerobic bacteriology is the difficulty in accurately precisely and rapidly identifying unknown isolates. Tn the proceedings of the Third International Symposium on Rapid Methods and Automation in Microbiology C. M. Moss said: " Chromatographic analysis is a new future for clinical microbiology" . 12 years past and so far it seems that this is an idea whose time has not get come but it close. Now two major advances that have brought the technology forword in terms ofmaking it appropriate for use in the clinical laboratory can aldo be cited. One is the development and implementation of fused silica capillary columns. In contrast to packed columns and those of'' greater width these columns allow reproducible recovery of hydroxey fatty acids with the same carbon chain length. The second advance is the efficient data processing afforded by modern microcomputer systems. On the other hand the practical steps for sample preparation also are an advance in the clinical laboratory. Chromatographic Analysis means mainly of analysis of fatty acids. The most common

  6. Embodiment and enculturation: the future of architectural design

    PubMed Central

    Mallgrave, Harry F.

    2015-01-01

    A half-century ago the Dutch architect Aldo van Eyck encouraged designers to think about “space and time” not as abstractions in themselves but rather as cultural events better approached through the medium of “place and occasion.” Van Eyck made this point on the basis of his own travels and through his extensive readings in cultural anthropology, and his prescience is only now acquiring the credibility that it deserves through the work of a multitude of interdisciplinary researchers. Phenomenologists argue that we are embodied organisms-acting-within-environments, and these inhabiting abodes are constructed of both material and cultural dimensions. We are thus preeminently social in our range of self-consciousness, and intensely ceremonial in every facet of our being. Evolutionary psychologists and anthropologists are currently locating the origin and development of our most basic social behaviors far in our pre-human past; neuroscientists are today modeling our social circuits in the deepest reaches of our brains. Architecture would gain much from an updated cultural theory grounded in these new models of human existence. PMID:26441773

  7. Toward the identification of liver toxicity markers: a proteome study in human cell culture and rats.

    PubMed

    Thome-Kromer, Birgit; Bonk, Ines; Klatt, Mathias; Nebrich, Grit; Taufmann, Marion; Bryant, Stewart; Wacker, Ulrich; Köpke, Andreas

    2003-10-01

    The effects of toxic and nontoxic compound treatments were investigated by high resolution custom developed 2-11 pH gradient NEPHGE (non equilibrium pH gradient electrophoresis) two-dimensional electrophoresis. Two models were compared: (i) in vivo rat and (ii) the human cell line HepG2, to test their suitability in a proteomics based approach to identify a toxicity marker. 163 and 321 proteins were identified from the rat liver and the HepG2 proteome. These represent various isoforms of 113 and 194 different NCBI annotated gene sequences, respectively. Nine compounds were selected to induce proteome variations associated with liver toxicity and metabolism. The rat liver proteome database consists of 78 gels, the HepG2 database of 52 gels. Variant proteins were assessed regarding their usefulness as a toxicity marker by evaluating their treatment specificity against multiple control treatments. Thirteen potential toxicity marker proteins were found in rat liver and eight in HepG2. Catalase and carbamoylphosphate synthetase-1 isoforms were found to be significantly changed after treatment by 4/4 and 3/4 toxic compounds in rat liver, respectively. Aldo-keto-reductase family 1, member C1 was implicated for 3/4 liver cell toxic compounds in HepG2. Our approach was able to differentiate the quality of potential toxicity markers and provided useful information for an ongoing characterization of more compounds in a wider number of toxicity classes. PMID:14625847

  8. Pathway of degradation of nitrilotriacetate by a Pseudomonas species.

    PubMed Central

    Firestone, M K; Tiedje, J M

    1978-01-01

    The pathway of degradation of nitrilotriacetate (NTA) was determined by using cell-free extracts and a 35-fold purification of NTA monooxygenase. The first step in the breakdown was an oxidative cleavage of the tertiary amine by the monooxygenase to form the aldo acid, glyoxylate, and the secondary amine, iminodiacetate (IDA). NTA N-oxide acted as a substrate analog for induction of the monooxygenase and was slowly metabolized by the enzyme, but was not an intermediate in the pathway. No intermediate before IDA was found, but an unstable alpha-hydroxy-NTA intermediate was postulated. IDA did undergo cleavage in the presence of the purified monooxygenase to give glyoxylate and glycine, but was not metabolized in cell-free extracts. Glyoxylate was further metabolized by cell-free extracts to yield CO2 and glycerate or glycine, products also found from NTA metabolism. Of the three bacterial isolates in which the NTA pathway has been studied, two strains, one isolated from a British soil and ours from a Michigan soil, appear to be almost identical. Images PMID:655711

  9. Detoxifying Enzymes at the Cross-Roads of Inflammation, Oxidative Stress, and Drug Hypersensitivity: Role of Glutathione Transferase P1-1 and Aldose Reductase.

    PubMed

    Sánchez-Gómez, Francisco J; Díez-Dacal, Beatriz; García-Martín, Elena; Agúndez, José A G; Pajares, María A; Pérez-Sala, Dolores

    2016-01-01

    Phase I and II enzymes are involved in the metabolism of endogenous reactive compounds as well as xenobiotics, including toxicants and drugs. Genotyping studies have established several drug metabolizing enzymes as markers for risk of drug hypersensitivity. However, other candidates are emerging that are involved in drug metabolism but also in the generation of danger or costimulatory signals. Enzymes such as aldo-keto reductases (AKR) and glutathione transferases (GST) metabolize prostaglandins and reactive aldehydes with proinflammatory activity, as well as drugs and/or their reactive metabolites. In addition, their metabolic activity can have important consequences for the cellular redox status, and impacts the inflammatory response as well as the balance of inflammatory mediators, which can modulate epigenetic factors and cooperate or interfere with drug-adduct formation. These enzymes are, in turn, targets for covalent modification and regulation by oxidative stress, inflammatory mediators, and drugs. Therefore, they constitute a platform for a complex set of interactions involving drug metabolism, protein haptenation, modulation of the inflammatory response, and/or generation of danger signals with implications in drug hypersensitivity reactions. Moreover, increasing evidence supports their involvement in allergic processes. Here, we will focus on GSTP1-1 and aldose reductase (AKR1B1) and provide a perspective for their involvement in drug hypersensitivity. PMID:27540362

  10. Differential expression of type 2 3α/type 5 17β-hydroxysteroid dehydrogenase (AKR1C3) in tumors of the central nervous system

    PubMed Central

    Park, Aubrey L; Lin, Hsueh-Kung; Yang, Qing; Sing, Chor Wing; Fan, Michael; Mapstone, Timothy B; Gross, Naina L; Gumerlock, Mary K; Martin, Michael D; Rabb, Craig H; Fung, Kar-Ming

    2010-01-01

    Human aldo-keto reductase (AKR) 1C3, type 2 3α-hydroxysteroid dehydrogenase (HSC)/ type 5 17β-HSD, is known to be involved in steroids, prostaglandins, and lipid aldehydes metabolism. The expression of AKR1C3 has been demonstrated in hormone-dependent normal tissues such as breast, endometrium, prostate, and testis; and de -regulated AKR1C3 expression has been shown in breast carcinoma, endometrial hyperplasia, endometrial carcinoma, and prostate carcinoma. AKR1C3 expression has also been demonstrated in hormone-independent normal tissues (renal tubules and urothelium) and neoplastic tissues (renal cell carcinoma, Wilm's tumor, and urothelial cell carcinoma). Extensive expression of AKR1C3 in normal and neoplastic as well as hormone-dependent and hormone-independent tissues indicates that AKR1C3 may have functions beyond steroid hormone metabolism. In this report, we describe a widespread expression of AKR1C3 in glial neoplasms and meningiomas, with limited expression in medulloblastoma and no expression in Schwannoma. These tumors, except meningioma, are not classically considered to be sex hormone-dependent or related brain tumors. The current results corroborate our earlier observations that AKR1C3 is expressed in both sex hormone-dependent and hormone-independent malignancies. Similar to AKR1C3 distribution in Wilm’s tumor, we also demonstrate that expression of AKR1C3 is reduced in tumors with embryonic phenotypes. PMID:21151387

  11. Methyl Jasmonate: Putative Mechanisms of Action on Cancer Cells Cycle, Metabolism, and Apoptosis

    PubMed Central

    Cesari, Italo Mario; Figueiredo Rodrigues, Mariana; Mendonça, Bruna dos Santos; Amôedo, Nivea Dias; Rumjanek, Franklin David

    2014-01-01

    Methyl jasmonate (MJ), an oxylipid that induces defense-related mechanisms in plants, has been shown to be active against cancer cells both in vitro and in vivo, without affecting normal cells. Here we review most of the described MJ activities in an attempt to get an integrated view and better understanding of its multifaceted modes of action. MJ (1) arrests cell cycle, inhibiting cell growth and proliferation, (2) causes cell death through the intrinsic/extrinsic proapoptotic, p53-independent apoptotic, and nonapoptotic (necrosis) pathways, (3) detaches hexokinase from the voltage-dependent anion channel, dissociating glycolytic and mitochondrial functions, decreasing the mitochondrial membrane potential, favoring cytochrome c release and ATP depletion, activating pro-apoptotic, and inactivating antiapoptotic proteins, (4) induces reactive oxygen species mediated responses, (5) stimulates MAPK-stress signaling and redifferentiation in leukemia cells, (6) inhibits overexpressed proinflammatory enzymes in cancer cells such as aldo-keto reductase 1 and 5-lipoxygenase, and (7) inhibits cell migration and shows antiangiogenic and antimetastatic activities. Finally, MJ may act as a chemosensitizer to some chemotherapics helping to overcome drug resistant. The complete lack of toxicity to normal cells and the rapidity by which MJ causes damage to cancer cells turn MJ into a promising anticancer agent that can be used alone or in combination with other agents. PMID:24648844

  12. Structure of conjugated polyketone reductase from Candida parapsilosis IFO 0708 reveals conformational changes for substrate recognition upon NADPH binding.

    PubMed

    Qin, Hui-Min; Yamamura, Akihiro; Miyakawa, Takuya; Kataoka, Michihiko; Nagai, Takahiro; Kitamura, Nahoko; Urano, Nobuyuki; Maruoka, Shintaro; Ohtsuka, Jun; Nagata, Koji; Shimizu, Sakayu; Tanokura, Masaru

    2014-01-01

    Conjugated polyketone reductase C2 (CPR-C2) from Candida parapsilosis IFO 0708, identified as a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent ketopantoyl lactone reductase, belongs to the aldo-keto reductase superfamily. This enzyme reduces ketopantoyl lactone to D-pantoyl lactone in a strictly stereospecific manner. To elucidate the structural basis of the substrate specificity, we determined the crystal structures of the apo CPR-C2 and CPR-C2/NADPH complex at 1.70 and 1.80 Å resolutions, respectively. CPR-C2 adopted a triose-phosphate isomerase barrel fold at the core of the structure. Binding with the cofactor NADPH induced conformational changes in which Thr27 and Lys28 moved 15 and 5.0 Å, respectively, in the close vicinity of the adenosine 2'-phosphate group of NADPH to form hydrogen bonds. Based on the comparison of the CPR-C2/NADPH structure with 3-α-hydroxysteroid dehydrogenase and mutation analyses, we constructed substrate binding models with ketopantoyl lactone, which provided insight into the substrate specificity by the cofactor-induced structure. The results will be useful for the rational design of CPR-C2 mutants targeted for use in the industrial manufacture of ketopantoyl lactone. PMID:23828603

  13. Multiple abiotic stress tolerance in Vigna mungo is altered by overexpression of ALDRXV4 gene via reactive carbonyl detoxification.

    PubMed

    Singh, Preeti; Kumar, Deepak; Sarin, Neera Bhalla

    2016-06-01

    Vigna mungo (blackgram) is an important leguminous pulse crop, which is grown for its protein rich edible seeds. Drought and salinity are the major abiotic stresses which adversely affect the growth and productivity of crop plants including blackgram. The ALDRXV4 belongs to the aldo-keto reductase superfamily of enzymes that catalyze the reduction of carbonyl metabolites in the cells and plays an important role in the osmoprotection and detoxification of the reactive carbonyl species. In the present study, we developed transgenic plants of V. mungo using Agrobacterium mediated transformation. The transgene integration was confirmed by Southern blot analysis whereas the expression was confirmed by RT-PCR, Western blot and enzyme activity. The T1 generation transgenic plants displayed improved tolerance to various environmental stresses, including drought, salt, methyl viologen and H2O2 induced oxidative stress. The increased aldose reductase activity, higher sorbitol content and less accumulation of the toxic metabolite, methylglyoxal in the transgenic lines under non-stress and stress (drought and salinity) conditions resulted in increased protection through maintenance of better photosynthetic efficiency, higher relative water content and less photooxidative damage. The accumulation of reactive oxygen species was remarkably decreased in the transgenic lines as compared with the wild type plants. This study of engineering multiple stress tolerance in blackgram, is the first report to date and this strategy for trait improvement is proposed to provide a novel germplasm for blackgram production on marginal lands. PMID:26956699

  14. Stereochemical inversion of (S)-reticuline by a cytochrome P450 fusion in opium poppy.

    PubMed

    Farrow, Scott C; Hagel, Jillian M; Beaudoin, Guillaume A W; Burns, Darcy C; Facchini, Peter J

    2015-09-01

    The gateway to morphine biosynthesis in opium poppy (Papaver somniferum) is the stereochemical inversion of (S)-reticuline since the enzyme yielding the first committed intermediate salutaridine is specific for (R)-reticuline. A fusion between a cytochrome P450 (CYP) and an aldo-keto reductase (AKR) catalyzes the S-to-R epimerization of reticuline via 1,2-dehydroreticuline. The reticuline epimerase (REPI) fusion was detected in opium poppy and in Papaver bracteatum, which accumulates thebaine. In contrast, orthologs encoding independent CYP and AKR enzymes catalyzing the respective synthesis and reduction of 1,2-dehydroreticuline were isolated from Papaver rhoeas, which does not accumulate morphinan alkaloids. An ancestral relationship between these enzymes is supported by a conservation of introns in the gene fusions and independent orthologs. Suppression of REPI transcripts using virus-induced gene silencing in opium poppy reduced levels of (R)-reticuline and morphinan alkaloids and increased the overall abundance of (S)-reticuline and its O-methylated derivatives. Discovery of REPI completes the isolation of genes responsible for known steps of morphine biosynthesis. PMID:26147354

  15. Intracrine Androgens Enhance Decidualization and Modulate Expression of Human Endometrial Receptivity Genes

    PubMed Central

    Gibson, Douglas A.; Simitsidellis, Ioannis; Cousins, Fiona L.; Critchley, Hilary O. D.; Saunders, Philippa T. K.

    2016-01-01

    The endometrium is a complex, steroid-dependent tissue that undergoes dynamic cyclical remodelling. Transformation of stromal fibroblasts (ESC) into specialised secretory cells (decidualization) is fundamental to the establishment of a receptive endometrial microenvironment which can support and maintain pregnancy. Androgen receptors (AR) are present in ESC; in other tissues local metabolism of ovarian and adrenal-derived androgens regulate AR-dependent gene expression. We hypothesised that altered expression/activity of androgen biosynthetic enzymes would regulate tissue availability of bioactive androgens and the process of decidualization. Primary human ESC were treated in vitro for 1–8 days with progesterone and cAMP (decidualized) in the presence or absence of the AR antagonist flutamide. Time and treatment-dependent changes in genes essential for a) intra-tissue biosynthesis of androgens (5α-reductase/SRD5A1, aldo-keto reductase family 1 member C3/AKR1C3), b) establishment of endometrial decidualization (IGFBP1, prolactin) and c) endometrial receptivity (SPP1, MAOA, EDNRB) were measured. Decidualization of ESC resulted in significant time-dependent changes in expression of AKR1C3 and SRD5A1 and secretion of T/DHT. Addition of flutamide significantly reduced secretion of IGFBP1 and prolactin and altered the expression of endometrial receptivity markers. Intracrine biosynthesis of endometrial androgens during decidualization may play a key role in endometrial receptivity and offer a novel target for fertility treatment. PMID:26817618

  16. Utilization of digital differential display to identify differentially expressed genes related to rumen development.

    PubMed

    Kato, Daichi; Suzuki, Yutaka; Haga, Satoshi; So, KyoungHa; Yamauchi, Eri; Nakano, Miwa; Ishizaki, Hiroshi; Choi, Kichoon; Katoh, Kazuo; Roh, Sang-Gun

    2016-04-01

    This study aimed to identify the genes associated with the development of the rumen epithelium by screening for candidate genes by digital differential display (DDD) in silico. Using DDD in NCBI's UniGene database, expressed sequence tag (EST)-based gene expression profiles were analyzed in rumen, reticulum, omasum, abomasum and other tissues in cattle. One hundred and ten candidate genes with high expression in the rumen were derived from a library of all tissues. The expression levels of 11 genes in all candidate genes were analyzed in the rumen, reticulum, omasum and abomasum of nine Japanese Black male calves (5-week-old pre-weaning: n = 3; 15-week-old weaned calves: n = 6). Among the 11 genes, only 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), aldo-keto reductase family 1, member C1-like (AKR1C1), and fatty acid binding protein 3 (FABP3) showed significant changes in the levels of gene expression in the rumen between the pre- and post-weaning of calves. These results indicate that DDD analysis in silico can be useful for screening candidate genes related to rumen development, and that the changes in expression levels of three genes in the rumen may have been caused by weaning, aging or both. © 2015 Japanese Society of Animal Science. PMID:26388291

  17. Promiscuity and diversity in 3-ketosteroid reductases.

    PubMed

    Penning, Trevor M; Chen, Mo; Jin, Yi

    2015-07-01

    Many steroid hormones contain a Δ(4)-3-ketosteroid functionality that undergoes sequential reduction by 5α- or 5β- steroid reductases to produce 5α- or 5β-dihydrosteroids; and a subsequent 3-keto-reduction to produce a series of isomeric tetrahydrosteroids. Apart from steroid 5α-reductase all the remaining enzymes involved in the two step reduction process in humans belong to the aldo-keto reductase (AKR) superfamily. The enzymes involved in 3-ketosteroid reduction are AKR1C1-AKR1C4. These enzymes are promiscuous and also catalyze 20-keto- and 17-keto-steroid reduction. Interest in these reactions exist since they regulate steroid hormone metabolism in the liver, and in steroid target tissues, they may regulate steroid hormone receptor occupancy. In addition many of the dihydrosteroids are not biologically inert. The same enzymes are also involved in the metabolism of synthetic steroids e.g., hormone replacement therapeutics, contraceptive agents and inhaled glucocorticoids, and may regulate drug efficacy at their cognate receptors. This article reviews these reactions and the structural basis for substrate diversity in AKR1C1-AKR1C4, ketosteroid reductases. This article is part of a Special Issue entitled 'Steroid/Sterol signaling'. PMID:25500069

  18. Involvement of an octose ketoreductase and two acyltransferases in the biosynthesis of paulomycins

    PubMed Central

    Li, Jine; Wang, Min; Ding, Yong; Tang, Yue; Zhang, Zhiguo; Chen, Yihua

    2016-01-01

    C-4 hydroxyethyl branched octoses have been observed in polysaccharides of several genera of gram negative bacteria and in various antibiotics produced by gram positive bacteria. The C-4 hydroxyethyl branch was proposed to be converted from C-4 acetyl branch by an uncharacterized ketoreduction step. Paulomycins (PAUs) are glycosylated antibiotics with potent inhibitory activity against gram positive bacteria and are structurally defined by its unique C-4′ hydroxyethyl branched paulomycose moiety. A novel aldo-keto-reductase, Pau7 was characterized as the enzyme catalyzing the stereospecific ketoreduction of 7′-keto of PAU E (1) to give the C-4′ hydroxyethyl branched paulomycose moiety of PAU F (2). An acyltransferase Pau6 further decorates the C-4′ hydroxyethyl branch of paulomycose moiety of 2 by attaching various fatty acyl chains to 7′-OH to generate diverse PAUs. In addition, another acyltransferase Pau24 was proposed to be responsible for the 13-O-acetylation of PAUs. PMID:26877148

  19. Expressions of ecological identity across the life span of eight environmental exemplars

    NASA Astrophysics Data System (ADS)

    Seydel, Jennifer

    While there is a substantial body of literature looking at various aspects of ecological identity and factors that influence it, there has been less work done on how an individual's ecological identity changes with time. Much of that work is limited to short segments of the life span (e.g. the impact of wilderness experiences). This dissertation attempts to address this perceived gap by investigating how the ecological identity of eight environmental exemplars changed during the course of his or her life. What has emerged from this qualitative grounded theory investigation of the lives and works of Charles Darwin, John Muir, Aldo Leopold, Marjory Stoneman Douglas, Hazel Wolf, Rachel Carson, James Lovelock and E.O. Wilson are five sequential expressions of ecological identity. These 'stages' serve as a framework to explain ecological identity as a developmental process, both fluid and continuous, rather than at) end product. The development of an ecological identity is traced, through the development of five cognitive foundations and their alignment with five emotional foundations that reflect a progression from a sensory interaction and a kinship bond with nature into a deep understanding of the interconnectedness of all aspects of the planet. The findings reveal the evolution of an ecological identity and suggest the importance of looking beyond content knowledge in the nurturing of ecological attitudes, values, and lifestyles.

  20. Transcriptomic responses of European flounder (Platichthys flesus) liver to a brominated flame retardant mixture.

    PubMed

    Williams, Tim D; Diab, Amer M; Gubbins, Matt; Collins, Catherine; Matejusova, Iveta; Kerr, Rose; Chipman, James K; Kuiper, Raoul; Vethaak, A Dick; George, Stephen G

    2013-10-15

    Male European flounder (Platichthys flesus) were exposed to a technical mixture of brominated diphenyl ethers (PDBEs, DE-71, Pentamix) that had been purified to remove contaminating dioxins. Controls were exposed to carrier solvent alone. Fish were exposed to decadally increasing concentrations of Pentamix via both sediment and spiked food. The GENIPOL P. flesus cDNA microarray, differentially expressed gene profiling (DEG) and quantitative PCR were employed to detect hepatic transcriptional differences between exposed fish and controls. Gene transcriptional changes were more sensitive to Pentamix exposure than biomarkers measured previously. Pentamix exposure induced transcripts coding for enzymes of xenobiotic metabolism (CYP1A, aldo-keto reductases) and elicited endocrine disruption (vitellogenin and thyroid hormone receptor alpha), with effects on CYP1A and VTG occurring at the highest exposure. Ontology analysis clearly showed dose-responsive changes indicative of oxidative stress, induction of mitochondrial dysfunction, and apoptosis. We conclude that exposure to PBDEs in both sediment and food has a significant adverse effect on a broad range of crucial biochemical processes in the livers of this widely distributed estuarine fish species, the flounder. PMID:23948077

  1. Targeting bacterial membranes: NMR spectroscopy characterization of substrate recognition and binding requirements of D-arabinose-5-phosphate isomerase.

    PubMed

    Airoldi, Cristina; Sommaruga, Silvia; Merlo, Silvia; Sperandeo, Paola; Cipolla, Laura; Polissi, Alessandra; Nicotra, Francesco

    2010-02-01

    Lipopolysaccharide (LPS) is an essential component of the outer membrane of gram-negative bacteria and consists of three elements: lipid A, the core oligosaccharide, and the O-antigen. The inner-core region is highly conserved and contains at least one residue of 3-deoxy-D-manno-octulosonate (Kdo). Arabinose-5-phosphate isomerase (API) is an aldo-keto isomerase catalyzing the reversible isomerization of D-ribulose-5-phosphate (Ru5P) to D-arabinose-5-phosphate (A5P), the first step of Kdo biosynthesis. By exploiting saturation transfer difference (STD) NMR spectroscopy, the structural requirements necessary for API substrate recognition and binding were identified, with the aim of designing new API inhibitors. In addition, simple experimental conditions for the STD experiments to perform a fast, robust, and efficient screening of small libraries of potential API inhibitors, allowing the identification of new potential leads, were set up. Due to the essential role of API enzymes in LPS biosynthesis and gram-negative bacteria survival, by exploiting these data, a new generation of potent antibacterial drugs could be developed. PMID:20039350

  2. Promiscuity and diversity in 3-ketosteroid reductases

    PubMed Central

    Penning, Trevor M.; Chen, Mo; Jin, Yi

    2014-01-01

    Many steroid hormones contain a Δ4-3-ketosteroid functionality that undergoes sequential reduction by 5α- or 5β- steroid reductases to produce 5α- or 5β-dihydrosteroids; and a subsequent 3-keto-reduction to produce a series of isomeric tetrahydrosteroids. Apart from steroid 5α-reductase all the remaining enzymes involved in the two step reduction process in humans belong to the aldo-keto reductase (AKR) superfamily. The enzymes involved in 3-ketosteroid reduction are AKR1C1–AKR1C4. These enzymes are promiscuous and also catalyze 20-keto- and 17-keto-steroid reduction. Interest in these reactions exist since they regulate steroid hormone metabolism in the liver, and in steroid target tissues, they may regulate steroid hormone receptor occupancy. In addition many of the dihydrosteroids are not biologically inert. The same enzymes are also involved in the metabolism of synthetic steroids e.g., hormone replacement therapeutics, contraceptive agents and inhaled glucocorticoids, and may regulate drug efficacy at their cognate receptors. This article reviews these reactions and the structural basis for substrate diversity in AKR1C1–AKR1C4, ketosteroid reductases. This article is part of a Special Issue entitled ‘Steroid/Sterol signaling’. PMID:25500069

  3. Age-Related Changes in Hepatic Activity and Expression of Detoxification Enzymes in Male Rats

    PubMed Central

    Vyskočilová, Erika; Szotáková, Barbora; Skálová, Lenka; Bártíková, Hana; Hlaváčová, Jitka

    2013-01-01

    Process of aging is accompanied by changes in the biotransformation of xenobiotics and impairment of normal cellular functions by free radicals. Therefore, this study was designed to determine age-related differences in the activities and/or expressions of selected drug-metabolizing and antioxidant enzymes in young and old rats. Specific activities of 8 drug-metabolizing enzymes and 4 antioxidant enzymes were assessed in hepatic subcellular fractions of 6-week-old and 21-month-old male Wistar rats. Protein expressions of carbonyl reductase 1 (CBR1) and glutathione S-transferase (GST) were determined using immunoblotting. Remarkable age-related decrease in specific activities of CYP2B, CYP3A, and UDP-glucuronosyl transferase was observed, whereas no changes in activities of CYP1A2, flavine monooxygenase, aldo-keto reductase 1C, and antioxidant enzymes with advancing age were found. On the other hand, specific activity of CBR1 and GST was 2.4 folds and 5.6 folds higher in the senescent rats compared with the young ones, respectively. Interindividual variability in CBR1 activity increased significantly with rising age. We suppose that elevated activities of GST and CBR1 may protect senescent rats against xenobiotic as well as eobiotic electrophiles and reactive carbonyls, but they may alter metabolism of drugs, which are CBR1 and especially GSTs substrates. PMID:23971034

  4. Scientists are From Venus, Journalists are From Mars: Bridging the Two Worlds

    NASA Astrophysics Data System (ADS)

    Baron, N.

    2006-12-01

    Media coverage of issues ranging from climate change to evolution often shapes public awareness and opinions about these topics and the science behind them. While scientists can play a critical social role as a resource for journalists and as a valuable information source for the public, they are often frustrated with how their work is portrayed in the press or choose to avoid public discussions completely. In order for science-based policy measures to succeed, scientists must engage in these public discussions and learn how to communicate more effectively - not only with each other, but also with the media, the public, and policy makers. This requires being able to put themselves in the shoes of their audiences. This presentation will provide insights into the world of journalism and offer practical steps that scientists can take to ensure that their research will register on the public's radar screen. Presenter Nancy Baron, Lead Communications Trainer for the Aldo Leopold Program (ALLP) and Ocean Science Outreach Director for COMPASS works closely with leading scientists to help them communicate the contents and importance of their work more effectively and make their science "news" without compromising scientific integrity or credibility.

  5. Lessons from the Trenches

    NASA Astrophysics Data System (ADS)

    Lubchenco, J.

    2006-12-01

    One of the most important roles of science is to inform the discussions and decisions of individuals and institutions. In a world that is changing rapidly, information is urgently needed to help citizens and leaders understand what's happening, what's causing changes, what the implications are and what are the likely consequences of various options. Most everyone agrees that decisions should be informed (not dictated) by scientific information, but achieving that goal has proven a challenge. Decision-makers need to have access to scientific information that is understandable, relevant, useable, current and credible. However, the science is complex, nuanced and difficult to communicate simply. Most scientists are ill equipped to speak in language that is non-technical. Many academic scientists are wary of talking to the press. Academia does not generally reward time spent doing outreach. As a consequence, others step into the breach and communicate their version of `the science.' All too often this means that vested interests spin, distort or cherry-pick information. The result is that decisions are made without good scientific knowledge and science is seen increasingly as a weapon, not as useful knowledge. The presentation will focus on how one program, the Aldo Leopold Leadership Program is training academic environmental scientists to be better communicators of their science to non-scientists. Lessons learned and suggestions for revolutionizing the communication of scientific information will be offered.

  6. Confessions of a Communications Junkie: Cliff Notes From the Science-Practice Interface

    NASA Astrophysics Data System (ADS)

    Moser, S. C.

    2006-12-01

    Graduate education in the sciences is - among other things - about learning a foreign language. Proficiency in disciplinary jargon and a strange sort of eloquence in speaking English without being understood by anyone outside one's small 'country of expertise' are among the requirements for entry into academe. Until very recently, the ability to translate one's quirky knowledge back into common language was not part of entraining scientists. Yet, increasingly, the interested public, policy-makers and resource managers, not to speak of science funders, demand that scientists illustrate that their science has societal relevance. Moreover, the urgency of several complex societal and environmental problems puts the onus on scientists to work with experts in other disciplines. This means that the ability to communicate effectively with those outside one's own disciplinary home is rapidly becoming an essential qualification of a 'good' scientist. My own journey from a disciplinary boundary crosser, to hobby communicator, to professional translator of science into English, to alumnae of various media trainings and Aldo Leopold Leadership Fellow, to researcher of the science-practice interface and expert in communication for social change will form the basis of this talk. It weaves together personal experience with scientific insights on why scientists should, why many don't, and how they could interact more effectively with members of a 'different tribe.'

  7. Landscapes of promise: An examination of students' journals written during a cross-cultural wilderness experience

    NASA Astrophysics Data System (ADS)

    Freund, Judith Ann

    1997-12-01

    This paper is an examination of nature journals written by ten American and ten Russian high school students during a cross-cultural exchange that provided experiences in selected national wilderness areas designated by the respective countries. The students participated in a backpacking excursion in the Lee Metcalf Wilderness Area of Montana in the summer of 1994, and a camping experience in the wilderness areas in the provincial region of Penza, Russia in the summer of 1995. The examination of the journals focuses on the following areas: aesthetic "peak" experiences; spiritual inspiration derived from experiences in nature; attitudes toward the preservation of wildlife; and environmental ethics. The students' attitudes toward the environment is compared using student-identified cultural values of both the Russian and the American students. Also discussed is the viability of the students' reflections as natural history journal-writing, with references to selected natural history authors, including Henry David Thoreau, Aldo Leopold and Anne Dillard. Because the experience focused on wilderness preservation students were invited to speculate about how to develop and reinforce essential attitudes that are respectful of ecology. Conclusions they reached included the necessity to economic security at some level and the notion that direct experience in the environment is essential to developing an attitude that will engender an ethics of caring within their--as well as other--cultural groups.

  8. Record-breaking early flowering in the eastern United States.

    PubMed

    Ellwood, Elizabeth R; Temple, Stanley A; Primack, Richard B; Bradley, Nina L; Davis, Charles C

    2013-01-01

    Flowering times are well-documented indicators of the ecological effects of climate change and are linked to numerous ecosystem processes and trophic interactions. Dozens of studies have shown that flowering times for many spring-flowering plants have become earlier as a result of recent climate change, but it is uncertain if flowering times will continue to advance as temperatures rise. Here, we used long-term flowering records initiated by Henry David Thoreau in 1852 and Aldo Leopold in 1935 to investigate this question. Our analyses demonstrate that record-breaking spring temperatures in 2010 and 2012 in Massachusetts, USA, and 2012 in Wisconsin, USA, resulted in the earliest flowering times in recorded history for dozens of spring-flowering plants of the eastern United States. These dramatic advances in spring flowering were successfully predicted by historical relationships between flowering and spring temperature spanning up to 161 years of ecological change. These results demonstrate that numerous temperate plant species have yet to show obvious signs of physiological constraints on phenological advancement in the face of climate change. PMID:23342001

  9. Phenology Across the LTER Network: Initial Findings, Future Directions

    NASA Astrophysics Data System (ADS)

    Henebry, G. M.

    2007-12-01

    Phenology is, in the words of Aldo Leopold, a "horizontal science" that cuts across and binds together multiple biological disciplines. It is a far-reaching but poorly understood aspect of the environmental sciences. Phenological research has been a component of the Long Term Ecological Research (LTER) Network at several sites over the years. However, it has not received the attention or resources to bring it to the forefront as an effective theme for interdisciplinary and cross-site synthesis. With the recent establishment of the USA National Phenology Network (USA-NPN), it is appropriate to assess the status of phenological knowledge across the LTER Network. A workshop funded by the LTER Network Office took place at the Sevilleta Field Station during February 26 to March 2, 2007. From the workshop three main products emerged: (1) an inventory of LTER phenology datasets, (2) establishment of a website to facilitate information interchange, and (3) a white paper recommending next steps for the LTER Network to engage the USA-NPN. This poster relates the findings and recommendations of the workshop, including a summary of phenologically explicit and phenologically implicit LTER datasets and illustrations of how the climatic envelopes described by simple weather variables can provide context for phenological comparisons within and across sites.

  10. Designing an Effective Environmental Education Program that Meets the Needs of Stakeholders: A Case Study of the High Lonesome Ranch in DeBeque, Colorado

    NASA Astrophysics Data System (ADS)

    Haley, Bethany M.

    A successful environmental education (EE) program is one that supports the mission of the parent organization, meets the needs of its audiences, and is effective at increasing environmental literacy. The High Lonesome Ranch (HLR), a private corporation that operates according to a mixed-use landscape model inspired by Aldo Leopold's land ethic, intends to develop an EE program that will operate within an associated nonprofit organization, the High Lonesome Institute (HLI), to further the mission of promoting a contemporary land ethic. Although HLR owners and staff are motivated to develop an EE program, there is currently no clear consensus regarding an overall vision for the program. The purpose of my thesis is to provide HLR owners and their advisors with a recommended design for the education program based on the missions of the HLR and HLI, sound EE theory, stakeholder feedback, and feasibility within the environment of the HLR. To accomplish this, I reviewed pertinent EE literature, reviewed the models used by existing EE programs, and undertook a two-pronged qualitative case study that gathered feedback from the major stakeholders in the HLR/HLI program. The case study included stakeholder interviews and a one-day facilitated charrette. Although feedback from stakeholders on specific program elements ranged widely, there was widespread support for an EE program at the HLR. From this research, I made a series of recommendations regarding how the HLR/HLI should proceed in development, specific program elements, and next steps in the process.

  11. Climate Literacy Ambassadors

    NASA Astrophysics Data System (ADS)

    Ackerman, S. A.; Mooney, M. E.

    2011-12-01

    The Climate Literacy Ambassadors program is a collaborative effort to advance climate literacy led by the Cooperative Institute of Meteorological Satellite Studies (CIMSS) at the University of Wisconsin-Madison. With support from NASA, CIMSS is coordinating a three-tiered program to train G6-12 teachers to be Ambassadors of Climate Literacy in their schools and communities. The complete training involves participation at a teacher workshop combined with web-based professional development content around Global and Regional Climate Change. The on-line course utilizes e-learning technology to clarify graphs and concepts from the 2007 Intergovernmental Panel on Climate Change Summary for Policy Makers with content intricately linked to the Climate Literacy: The Essential Principles of Climate Science. Educators who take the course for credit can develop lesson plans or opt for a project of their choosing. This session will showcase select lesson plans and projects, ranging from a district-wide action plan that engaged dozens of teachers to Ambassadors volunteering at the Aldo Leopold Climate Change Nature Center to a teacher who tested a GLOBE Student Climate Research Campaign (SCRC) learning project with plans to participate in the SCRC program. Along with sharing successes from the CIMSS Climate Literacy Ambassadors project, we will share lessons learned related to the challenges of sustaining on-line virtual educator communities.

  12. Beyond positivist ecology: toward an integrated ecological ethics.

    PubMed

    Norton, Bryan G

    2008-12-01

    A post-positivist understanding of ecological science and the call for an "ecological ethic" indicate the need for a radically new approach to evaluating environmental change. The positivist view of science cannot capture the essence of environmental sciences because the recent work of "reflexive" ecological modelers shows that this requires a reconceptualization of the way in which values and ecological models interact in scientific process. Reflexive modelers are ecological modelers who believe it is appropriate for ecologists to examine the motives for their choices in developing models; this self-reflexive approach opens the door to a new way of integrating values into public discourse and to a more comprehensive approach to evaluating ecological change. This reflexive building of ecological models is introduced through the transformative simile of Aldo Leopold, which shows that learning to "think like a mountain" involves a shift in both ecological modeling and in values and responsibility. An adequate, interdisciplinary approach to ecological valuation, requires a re-framing of the evaluation questions in entirely new ways, i.e., a review of the current status of interdisciplinary value theory with respect to ecological values reveals that neither of the widely accepted theories of environmental value-neither economic utilitarianism nor intrinsic value theory (environmental ethics)-provides a foundation for an ecologically sensitive evaluation process. Thus, a new, ecologically sensitive, and more comprehensive approach to evaluating ecological change would include an examination of the metaphors that motivate the models used to describe environmental change. PMID:18946726

  13. Record-Breaking Early Flowering in the Eastern United States

    PubMed Central

    Ellwood, Elizabeth R.; Temple, Stanley A.; Primack, Richard B.; Davis, Charles C.

    2013-01-01

    Flowering times are well-documented indicators of the ecological effects of climate change and are linked to numerous ecosystem processes and trophic interactions. Dozens of studies have shown that flowering times for many spring-flowering plants have become earlier as a result of recent climate change, but it is uncertain if flowering times will continue to advance as temperatures rise. Here, we used long-term flowering records initiated by Henry David Thoreau in 1852 and Aldo Leopold in 1935 to investigate this question. Our analyses demonstrate that record-breaking spring temperatures in 2010 and 2012 in Massachusetts, USA, and 2012 in Wisconsin, USA, resulted in the earliest flowering times in recorded history for dozens of spring-flowering plants of the eastern United States. These dramatic advances in spring flowering were successfully predicted by historical relationships between flowering and spring temperature spanning up to 161 years of ecological change. These results demonstrate that numerous temperate plant species have yet to show obvious signs of physiological constraints on phenological advancement in the face of climate change. PMID:23342001

  14. Modernizing Natural History: Berkeley's Museum of Vertebrate Zoology in Transition.

    PubMed

    Sunderland, Mary E

    2013-01-01

    Throughout the twentieth century calls to modernize natural history motivated a range of responses. It was unclear how research in natural history museums would participate in the significant technological and conceptual changes that were occurring in the life sciences. By the 1960s, the Museum of Vertebrate Zoology at the University of California, Berkeley, was among the few university-based natural history museums that were able to maintain their specimen collections and support active research. The MVZ therefore provides a window to the modernization of natural history. This paper concentrates on the directorial transitions that occurred at the MVZ between 1965 and 1971. During this period, the MVZ had four directors: Alden H. Miller (Director 1940-1965), an ornithologist; Aldo Starker Leopold (Acting Director 1965-1966), a conservationist and wildlife biologist; Oliver P. Pearson (Director 1966-1971), a physiologist and mammalogist; and David B. Wake (Director 1971-1998), a morphologist, developmental biologist, and herpetologist. The paper explores how a diversity of overlapping modernization strategies, including hiring new faculty, building infrastructure to study live animals, establishing new kinds of collections, and building modern laboratories combined to maintain collections at the MVZ's core. The paper examines the tensions between the different modernization strategies to inform an analysis of how and why some changes were institutionalized while others were short-lived. By exploring the modernization of collections-based research, this paper emphasizes the importance of collections in the transformation of the life sciences. PMID:22990484

  15. Ecological sustainability as the fourth landmark in the development of conservation ethics.

    PubMed

    White, Peter S; Tuttle, Julie P

    2013-10-01

    Aldo Leopold, in "The Land Ethic," made 2 important contributions to conservation ethics: he emphasized the community and ecosystem levels of organization and he explicitly included people as members of the biotic community. Leopold's writings remain eloquent, inspirational, and influential, but the ideas he describes are inherently complex, and ecological science has continued to evolve since "The Land Ethic" was published in 1949. We used 4 sets of quotations from Leopold's essays to develop our commentary on the meaning of and challenges in interpreting his work and to explore the ongoing development of conservation ethics: the "A-B cleavage" (Leopold's description of the contrast between utilitarian value versus a broader definition of value in nature), "land health" and the rightness of human action, the right of all species to continued existence in natural populations "at least in spots," and humans as "plain member[s] and citizen[s]" of the "land-community." We define the broader function of land and land health in "The Land Ethic" as including completeness, dynamic stability, and self-renewal in a way that incorporates the needs of humans and all other species. We argue that the consequences of implementing Leopold's land ethic include multiple conservation goals nested within an overall systems approach and that conservation science must clarify the implications of Leopold's ethic by quantitatively investigating and defining large-scale, system-level ecological sustainability. At this scale, land use will encompass areas ranging from large expanses of wilderness to areas dominated by humans. PMID:24033797

  16. Development of a CART Model to Predict the Synthesis of Cardiotoxic Daunorubicinol in Heart Tissue Samples From Donors With and Without Down Syndrome.

    PubMed

    Hoefer, Carrie C; Blair, Rachael Hageman; Blanco, Javier G

    2016-06-01

    Daunorubicin (DAUN) and doxorubicin (DOX) are used to treat a variety of cancers. The use of DAUN and DOX is hampered by the development of cardiotoxicity. Clinical evidence suggests that patients with leukemia and Down syndrome are at increased risk for anthracycline-related cardiotoxicity. Carbonyl reductases and aldo-keto reductases (AKRs) catalyze the reduction of DAUN and DOX into cardiotoxic C-13 alcohol metabolites. Anthracyclines also exert cardiotoxicity by triggering mitochondrial dysfunction. In recent studies, a collection of heart samples from donors with and without Down syndrome was used to investigate determinants for anthracycline-related cardiotoxicity including cardiac daunorubicin reductase activity (DA), carbonyl reductase/AKRs protein expression, mitochondrial DNA content (mtDNA), and AKR7A2 DNA methylation status. In this study, the available demographic, biochemical, genetic, and epigenetic data were integrated through classification and regression trees analysis with the aim of pinpointing the most relevant variables for the synthesis of cardiotoxic daunorubicinol (i.e., DA). Seventeen variables were considered as potential predictors. Leave-one-out-cross-validation was performed for model selection and to estimate the generalization error. The classification and regression trees analysis model and variable importance measures suggest that cardiac mtDNA content, mtDNA(4977) deletion frequency, and AKR7A2 protein content are the most important variables in determining DA. PMID:27112290

  17. Chronic Hypoxia-Inducible Transcription Factor-2 Activation Stably Transforms Juxtaglomerular Renin Cells into Fibroblast-Like Cells In Vivo

    PubMed Central

    Gerl, Katharina; Karger, Christian; Schwarzensteiner, Ilona; Kurtz, Armin

    2015-01-01

    On the basis of previous observations that deletion of the von Hippel–Lindau protein (pVHL) in juxtaglomerular (JG) cells of the kidney suppresses renin and induces erythropoietin expression, this study aimed to characterize the events underlying this striking change of hormone expression. We found that renin cell-specific deletion of pVHL in mice leads to a phenotype switch in JG cells, from a cuboid and multiple vesicle-containing form into a flat and elongated form without vesicles. This shift of cell phenotype was accompanied by the disappearance of marker proteins for renin cells (e.g., aldo-keto reductase family 1, member 7 and connexin 40) and by the appearance of markers of fibroblast-like cells (e.g., collagen I, ecto-5′-nucleotidase, and PDGF receptor-β). Furthermore, hypoxia-inducible transcription factor-2α (HIF-2α) protein constitutively accumulated in these transformed cells. Codeletion of pVHL and HIF-2α in JG cells completely prevented the phenotypic changes. Similar to renin expression in normal JG cells, angiotensin II negatively regulated erythropoietin expression in the transformed cells. In summary, chronic activation of HIF-2 in renal JG cells leads to a reprogramming of the cells into fibroblast-like cells resembling native erythropoietin-producing cells located in the tubulointerstitium. PMID:25071089

  18. Identification and Validation of HCC-specific Gene Transcriptional Signature for Tumor Antigen Discovery

    PubMed Central

    Petrizzo, Annacarmen; Caruso, Francesca Pia; Tagliamonte, Maria; Tornesello, Maria Lina; Ceccarelli, Michele; Costa, Valerio; Aprile, Marianna; Esposito, Roberta; Ciliberto, Gennaro; Buonaguro, Franco M.; Buonaguro, Luigi

    2016-01-01

    A novel two-step bioinformatics strategy was applied for identification of signatures with therapeutic implications in hepatitis-associated HCC. Transcriptional profiles from HBV- and HCV-associated HCC samples were compared with non-tumor liver controls. Resulting HCC modulated genes were subsequently compared with different non-tumor tissue samples. Two related signatures were identified, namely “HCC-associated” and “HCC-specific”. Expression data were validated by RNA-Seq analysis carried out on unrelated HCC samples and protein expression was confirmed according to The Human Protein Atlas" (http://proteinatlas.org/), a public repository of immunohistochemistry data. Among all, aldo-keto reductase family 1 member B10, and IGF2 mRNA-binding protein 3 were found strictly HCC-specific with no expression in 18/20 normal tissues. Target peptides for vaccine design were predicted for both proteins associated with the most prevalent HLA-class I and II alleles. The described novel strategy showed to be feasible for identification of HCC-specific proteins as highly potential target for HCC immunotherapy. PMID:27387388

  19. 36th International Conference on High Energy Physics

    NASA Astrophysics Data System (ADS)

    The Australian particle physics community was honoured to host the 36th ICHEP conference in 2012 in Melbourne. This conference has long been the reference event for our international community. The announcement of the discovery of the Higgs boson at the LHC was a major highlight, with huge international press coverage. ICHEP2012 was described by CERN Director-General, Professor Rolf Heuer, as a landmark conference for our field. In additional to the Higgs announcement, important results from neutrino physics, from flavour physics, and from physics beyond the standard model also provided great interest. There were also updates on key accelerator developments such as the new B-factories, plans for the LHC upgrade, neutrino facilities and associated detector developments. ICHEP2012 exceeded the promise expected of the key conference for our field, and really did provide a reference point for the future. Many thanks to the contribution reviewers: Andy Bakich, Csaba Balazs, Nicole Bell, Catherine Buchanan, Will Crump, Cameron Cuthbert, Ben Farmer, Sudhir Gupta, Elliot Hutchison, Paul Jackson, Geng-Yuan Jeng, Archil Kobakhidze, Doyoun Kim, Tong Li, Antonio Limosani (Head Editor), Kristian McDonald, Nikhul Patel, Aldo Saavedra, Mark Scarcella, Geoff Taylor, Ian Watson, Graham White, Tony Williams and Bruce Yabsley.

  20. Hypothalamic thermal stimulation modulates vasopressin release in hyperosmotically stimulated rabbits.

    PubMed

    Keil, R; Gerstberger, R; Simon, E

    1994-10-01

    Under thermoneutral conditions conscious rabbits received systemic infusions of NaCl as hypertonic solution (90 mueq.min-1.kg body wt-1), which raised their plasma osmolality from 283 to 312 mosmol/kgH2O. Rabbits receiving isotonic saline served as controls. Hypertonic stimulation induced a 60% reduction of both respiratory frequency and evaporative water loss. Rectal temperature rose by 0.4 degrees C despite enhanced peripheral vasodilation as indicated by increased ear skin temperature. Plasma vasopressin (AVP), aldosterone (ALDO), and corticosterone (COR) were significantly elevated from 6 to 16 pg/ml, 90 to 180 pg/ml, and 17 to 40 ng/ml, respectively. To elucidate the importance of central temperature for AVP and adrenal corticosteroid release, hypothalamic thermal stimulations (20 min) were superimposed during established iso- and hyperosmotic steady-state conditions. Different from isosmotic controls, hyperosmotic animals responded to hypothalamic cooling (37 degrees C) with a significant decrease in plasma AVP from 16 to 13 pg/ml and to hypothalamic warming (41 degrees C) with a significant rise from 16 to 19 pg/ml. A weak temperature effect on COR release was also disclosed, especially of hypothalamic cooling, which significantly lowered plasma COR from 42 to 34 ng/ml. These results provide evidence for positive local temperature coefficients of hypothalamic control of AVP release and suggest a similar property also for the control of COR release by the hypothalamo-adenohypophysial axis. PMID:7943420

  1. Preliminary Statistics from the NASA Alphasat Beacon Receiver in Milan, Italy

    NASA Technical Reports Server (NTRS)

    Zemba, Michael J.; Nessel, James A.; Morse, Jacquelynne R.

    2015-01-01

    NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have initiated a joint propagation campaign within the framework of the Alphasat propagation experiment to characterize rain attenuation, scintillation, and gaseous absorption effects of the atmosphere in the 40 GHz band. NASA GRC has developed and installed a KQ-band (20-40 GHz) beacon receiver at the POLIMI campus in Milan, Italy, which receives the 20-40 GHz signals broadcast from the Alphasat Aldo Paraboni TDP5 beacon payload. The primary goal of these measurements is to develop a physical model to improve predictions of communications systems performance within the Q-band. Herein, we provide an overview of the design and data calibration procedure, and present 6 months of preliminary statistics of the NASA propagation terminal, which has been installed and operating in Milan since May 2014. The Q-band receiver has demonstrated a dynamic range of 40 dB at an 8-Hz sampling rate. A weather station with an optical disdrometer is also installed to characterize rain drop size distribution for correlation with physical based models

  2. Preliminary Statistics from the NASA Alphasat Beacon Receiver in Milan, Italy

    NASA Technical Reports Server (NTRS)

    Nessel, James; Zemba, Michael; Morse, Jacquelynne; Luini, Lorenzo; Riva, Carlo

    2015-01-01

    NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have initiated a joint propagation campaign within the framework of the Alphasat propagation experiment to characterize rain attenuation, scintillation, and gaseous absorption effects of the atmosphere in the 40 gigahertz band. NASA GRC has developed and installed a K/Q-band (20/40 gigahertz) beacon receiver at the POLIMI campus in Milan, Italy, which receives the 20/40 gigahertz signals broadcast from the Alphasat Aldo Paraboni TDP no. 5 beacon payload. The primary goal of these measurements is to develop a physical model to improve predictions of communications systems performance within the Q-band. Herein, we provide an overview of the design and data calibration procedure, and present 6 months of preliminary statistics of the NASA propagation terminal, which has been installed and operating in Milan since May 2014. The Q-band receiver has demonstrated a dynamic range of 40 decibels at an 8-hertz sampling rate. A weather station with an optical disdrometer is also installed to characterize rain drop size distribution for correlation with physical based models.

  3. Ibn nafis - a forgotten genius in the discovery of pulmonary blood circulation.

    PubMed

    Akmal, M; Zulkifle, M; Ansari, Ah

    2010-03-01

    Scientific theories take centuries to come into existence and they keep on evolving. Uncountable intellectual minds work on these theories; some fail to do anything about it; some add a little after tremendous efforts, and some people give remarkable and unforgettable contribution.As far as credit is concerned, the person who is able to prove the theory by his facts and who clears the maximum doubts by his observations, experimentations, facts and reasoning, gets the credit for that theory, and this should be done with honesty.The theory of pulmonary circulation took more than 2000 years to come into existence as we know it today. With the passage of time different people were given credit. Some say that it was given to Galen; some say it was Michael Servetus; others say that Realdus Columbus was the real discoverer; some gave the credit to Ibn Nafis, and finally people gave the credit to William Harvey. But after the rediscovery of Ibn Nafis' manuscript no.62243 titled Sharah al Tashreeh al Qanoon, or "Commentary on the anatomy of Canon of Avicenna" in 1924 AD in Europe, it became clear that Ibn Nafis had described the pulmonary circulation almost 300 years before Harvey, and the historians like Aldo Mieli, Max Mayrhoff, Edward Coppola etc. clearly state that Ibn Nafis is the real discoverer of the pulmonary circulation and that he should be given the credit for the discovery of the pulmonary circulation. PMID:21042463

  4. Phenotypic and metabolic investigation of a CSF-1R kinase receptor inhibitor (BLZ945) and its pharmacologically active metabolite.

    PubMed

    Krauser, Joel A; Jin, Yi; Walles, Markus; Pfaar, Ulrike; Sutton, James; Wiesmann, Marion; Graf, Daniel; Pflimlin-Fritschy, Veronique; Wolf, Thierry; Camenisch, Gian; Swart, Piet

    2015-02-01

    1. 4-[2((1R,2R)-2-Hydroxycyclohexylamino)-benzothiazol-6-yloxyl]-pyridine-2-carboxylic acid methylamide (BLZ945) is a small molecule inhibitor of CSF-1R kinase activity within osteoclasts designed to prevent skeletal related events in metastatic disease. Key metabolites were enzymatically and structurally characterized to understand the metabolic fate of BLZ945 and pharmacological implications. The relative intrinsic clearances for metabolites were derived from in vitro studies using human hepatocytes, microsomes and phenotyped with recombinant P450 enzymes. 2. Formation of a pharmacologically active metabolite (M9) was observed in human hepatocytes. The M9 metabolite is a structural isomer (diastereomer) of BLZ945 and is about 4-fold less potent. This isomer was enzymatically formed via P450 oxidation of the BLZ945 hydroxyl group, followed by aldo-keto reduction to the alcohol (M9). 3. Two reaction phenotyping approaches based on fractional clearances were applied to BLZ945 using hepatocytes and liver microsomes. The fraction metabolized (fm) or contribution ratio was determined for each metabolic reaction type (oxidation, glucuronidation or isomerization) as well as for each metabolite. The results quantitatively illustrate contribution ratios of the involved enzymes and pathways, e.g. the isomerization to metabolite M9 accounted for 24% intrinsic clearance in human hepatocytes. In summary, contribution ratios for the Phase I and Phase II pathways can be determined in hepatocytes. PMID:25180976

  5. Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells

    PubMed Central

    Seo, Dong-Cheol; Sung, Ji-Min; Cho, Hee-Jung; Yi, Hee; Seo, Kun-Ho; Choi, In-Soo; Kim, Dong-Ku; Kim, Jin-Suk; El-Aty AM, Abd; Shin, Ho-Chul

    2007-01-01

    Background The studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications. This study was carried out to investigate the gene expression profile of CSCs in human lung adenocarcinoma A549 cells. Results We isolated CSCs from A549 cell line of which side population (SP) phenotype revealed several stem cell properties. After staining the cell line with Hoechst 33342 dye, the SP and non-side population (non-SP) cells were sorted using flow cytometric analysis. The mRNA expression profiles were measured using an Affymetrix GeneChip® oligonucleotide array. Among the sixty one differentially expressed genes, the twelve genes inclusive three poor prognostic genes; Aldo-keto reductase family 1, member C1/C2 (AKR1C1/C2), Transmembrane 4 L six family member 1 nuclear receptor (TM4SF1), and Nuclear receptor subfamily 0, group B, member 1 (NR0B1) were significantly up-regulated in SP compared to non-SP cells. Conclusion This is the first report indicating the differences of gene expression pattern between SP and non-SP cells in A549 cells. We suggest that the up-regulations of the genes AKR1C1/C2, TM4SF1 and NR0B1 in SP of human adenocarcinoma A549 cells could be a target of poor prognosis in anti-cancer therapy. PMID:18034892

  6. Sulforaphane promotes murine hair growth by accelerating the degradation of dihydrotestosterone.

    PubMed

    Sasaki, Mari; Shinozaki, Shohei; Shimokado, Kentaro

    2016-03-25

    Dihydrotestosterone (DHT) causes the regression of human hair follicles in the parietal scalp, leading to androgenic alopecia (AGA). Sulforaphane (SFN) increases the expression of DHT degrading enzymes, such as 3α-hydroxysteroid dehydrogenases (3α-HSDs), and, therefore, SFN treatment may improve AGA. To determine the effects of SFN on hair growth, we administered SFN (10 mg/kg BW, IP) or vehicle (DMSO) to ob/ob mice for six weeks and examined hair regeneration and the plasma levels of testosterone and DHT. We also tested the effects of SFN on the expression of two forms of 3α-HSD, aldo-keto reductase 1c21 and dehydrogenase/reductase (SDR family) member 9, both in vitro and in vivo. SNF significantly enhanced hair regeneration in ob/ob mice. The mice treated with SFN showed lower plasma levels of testosterone and DHT than those treated with vehicle. SFN increased the mRNA and protein levels of the two forms of 3α-HSD in the liver of the mice and in cultured murine hepatocyte Hepa1c1c7 cells. These results suggest that SFN treatment increases the amount of 3α-HSDs in the liver, accelerates the degradation of blood DHT, and subsequently blocks the suppression of hair growth by DHT. PMID:26923074

  7. IBN NAFIS – A FORGOTTEN GENIUS IN THE DISCOVERY OF PULMONARY BLOOD CIRCULATION

    PubMed Central

    Akmal, M; Zulkifle, M; Ansari, AH

    2010-01-01

    Scientific theories take centuries to come into existence and they keep on evolving. Uncountable intellectual minds work on these theories; some fail to do anything about it; some add a little after tremendous efforts, and some people give remarkable and unforgettable contribution. As far as credit is concerned, the person who is able to prove the theory by his facts and who clears the maximum doubts by his observations, experimentations, facts and reasoning, gets the credit for that theory, and this should be done with honesty. The theory of pulmonary circulation took more than 2000 years to come into existence as we know it today. With the passage of time different people were given credit. Some say that it was given to Galen; some say it was Michael Servetus; others say that Realdus Columbus was the real discoverer; some gave the credit to Ibn Nafis, and finally people gave the credit to William Harvey. But after the rediscovery of Ibn Nafis’ manuscript no.62243 titled Sharah al Tashreeh al Qanoon, or “Commentary on the anatomy of Canon of Avicenna” in 1924 AD in Europe, it became clear that Ibn Nafis had described the pulmonary circulation almost 300 years before Harvey, and the historians like Aldo Mieli, Max Mayrhoff, Edward Coppola etc. clearly state that Ibn Nafis is the real discoverer of the pulmonary circulation and that he should be given the credit for the discovery of the pulmonary circulation. PMID:21042463

  8. AKR1C3 as a target in castrate resistant prostate cancer.

    PubMed

    Adeniji, Adegoke O; Chen, Mo; Penning, Trevor M

    2013-09-01

    Aberrant androgen receptor (AR) activation is the major driver of castrate resistant prostate cancer (CRPC). CRPC is ultimately fatal and more therapeutic agents are needed to treat this disease. Compounds that target the androgen axis by inhibiting androgen biosynthesis and or AR signaling are potential candidates for use in CRPC treatment and are currently being pursued aggressively. Aldo-keto reductase 1C3 (AKR1C3) plays a pivotal role in androgen biosynthesis within the prostate. It catalyzes the 17-ketoreduction of weak androgen precursors to give testosterone and 5α-dihydrotestosterone. AKR1C3 expression and activity has been implicated in the development of CRPC, making it a rational target. Selective inhibition of AKR1C3 will be important, however, due to the presence of closely related isoforms, AKR1C1 and AKR1C2 that are also involved in androgen inactivation. We examine the evidence that supports the vital role of AKR1C3 in CRPC and recent developments in the discovery of potent and selective AKR1C3 inhibitors. This article is part of a Special Issue entitled 'CSR 2013'. PMID:23748150

  9. The Moon as a Laser-ranged Test Body for General Relativity and New Gravitational Physics

    NASA Astrophysics Data System (ADS)

    Dell'Agnello, Simone; Currie, Douglas

    Since the 1970s Lunar Laser Ranging (LLR) to the Apollo/Lunokhod Cube Corner Retroreflector (CCR) Arrays supplied some of the best tests of General Relativity (GR): possible changes in the gravitational constant, gravitational self-energy (PPN parameter beta), weak equivalence principle, geodetic precession, inverse-square force-law. Secondly, LLR has provided significant information on the composition of the deep interior of the Moon. LLR physics analysis also allows to set constraints on extensions of GR (like spacetime torsion) and, possibly, on new gravitational physics which may explain the gravitational universe without Dark Matter and Dark Energy (like, for example, Non-Minimally Coupled gravity, NMC). LLR is the only Apollo/Lunokhod experiment still in operation, since 45 years. In the 1970s Apollo/Lunokohd LLR Arrays contributed a negligible fraction of the ranging error budget. Since the ranging capabilities of ground stations improved by more than two orders of magnitude, now, because of the lunar librations, Apollo/Lunokhod CCR arrays dominate the error budget. With the US/Italy project "LLRRA21/MoonLIGHT (Lunar Laser Ranging Retroreflector Array for the 21st century / Moon Laser Instrumentation for General relativity High accuracy Tests)", University of Maryland and INFN-LNF developed and tested a next-generation LLR payload made by a single, large CCR (100 mm diameter), unaffected by the effect of librations. In fact, we will show that MoonLIGHT reflectors will improve the LLR accuracy by a factor of ten to one hundred in a few years. INFN-LNF also developed a laser retroreflector micropayload to be deployed on the lunar surface to be laser-ranged by lunar orbiters. The latter micropayload will further extend the physics reach of Apollo, Lunokhod and MoonLIGHT CCRs to improve all precision tests of GR and new gravitational physics using LLR data. As an added value for the LRR and SLR (Satellite Laser ranging) disciplines INFN-LNF built and is

  10. PREFACE: XVth International Conference on Calorimetry in High Energy Physics (CALOR2012)

    NASA Astrophysics Data System (ADS)

    Akchurin, Nural

    2012-12-01

    The XVth International Conference on Calorimetry in High Energy Physics, CALOR2012, was held in Santa Fe, New Mexico from 4-8 June 2012. The series of conferences on calorimetry started in 1990 at Fermilab, and they have been the premier event for calorimeter aficionados, a trend that CALOR2012 upheld. This year, several presentations focused on the status of the major calorimeter systems, especially at the LHC. Discussions on new and developing techniques in calorimetry took a full day. Excellent updates on uses of calorimeters or about ideas that are deeply rooted in particle physics calorimetry in astrophysics and neutrino physics were followed by talks on algorithms and special triggers that rely on calorimeters. Finally, discussions of promising current developments and ongoing R&D work for future calorimeters capped the conference. The field of calorimetry is alive and well, as evidenced by the more than 100 attendees and the excellent quality of over 80 presentations. You will find the written contributions in this volume. The presentations can be found at calor2012.ttu.edu. The first day of the conference was dedicated to the LHC. In two invited talks, Guillaume Unal (CERN) and Tommaso Tabarelli de Fatis (Universita' & INFN Milano Bicocca) discussed the critical role electromagnetic calorimeters play in the hunt for the Standard Model Higgs boson in ATLAS and CMS, respectively. The enhanced sensitivity for light Higgs in the two-gamma decay channel renders electromagnetic calorimeters indispensible. Much of the higher mass region was already excluded for the SM Higgs by the time of this conference, and after less than a month, on 4 July, CERN announced the discovery of a new boson at 125 GeV, a particle that seems consistent with the Higgs particle so far. Once again, without the electromagnetic calorimeters, this would not have been possible. Professor Geoffrey West from the Santa Fe Institute gave the keynote address. His talk, 'Universal Scaling Laws

  11. Earth Sciences data access and preservation with gLibrary

    NASA Astrophysics Data System (ADS)

    Guidetti, Veronica; Calanducci, Antonio

    2010-05-01

    ESA-ESRIN, the European Space Agency Centre for Earth Observation (EO), is the largest European EO data provider and operates as the reference European centre for EO payload data exploitation. EO data acquired from space have become powerful scientific tools to enable better understanding and management of the Earth and its resources. Large international initiatives such as GMES and GEO, supported by the European Commission, focus on coordinating international efforts to environmental monitoring, i.e. to provide political and technical solutions to global issues, such as climate change, global environment monitoring, management of natural resources and humanitarian response. Since the time-span of EO data archives extends from a few years to decades, their value as scientific time-series increases considerably, especially for the topic of global change. It will be soon necessary to re-analyse on global scale the information currently locked inside large thematic archives. Future research in the field of Earth Sciences is of invaluable importance: to carry it on researchers worldwide must be enabled to find and access data of interest in a quick and easy way. At present, several thousands of scientists, principal investigators and operators, access EO missions' metadata, data and derived information on a daily basis. Main objectives may be to study the global climate change, to check the status of the instrument on-board and the quality of EO data. There is a huge worldwide scientific community calling for the need to keep EO data accessible without time constrains, easily and quickly. In collaboration with ESA-ESRIN, INFN, the National Institute for Nuclear Physics, is implementing a demonstrative use case where satellite remote sensing data, including in-situ data and other kind of digital assets, are made available to the scientific community via gLibrary (https://glibrary.ct.infn.it), the INFN digital library platform. gLibrary can be used to store, organise

  12. PREFACE: XIII International Conference on Calorimetry in High Energy Physics (CALOR 2008)

    NASA Astrophysics Data System (ADS)

    Livan, Michele

    2009-07-01

    presentations, now reported in these proceedings, that were debated in stimulating and fruitful discussions. Outside of the Workshop, the participants were able to visit the historical Halls and Museum of the University, whose foundation dates back to the year 1361, and to enjoy a visit to the Certosa, a Carthusian monastery renowned for its exuberant architecture. Pavia welcomed the Conference participants by opening the doors of the Town Hall and offering a reception during which the Mayor's address underlined the importance of research and its applications in modern society. The successful organization and the smooth running of the Conference is due to many people and Institutions. We gratefully acknowledge the financial support of the Istituto Nazionale di Fisica Nucleare (INFN), the Department of Nuclear and Theoretical Physics and the University of Pavia, that made the Workshop possible, together with the contribution of our sponsors. The University also opened some rooms of the Chancellor's suite for the lunch and coffee breaks, and hosted the Conference Secretariat. We would like to express our deepest gratitude to the INFN and Department technical staff, who helped to prepare the Hall for the Conference and to provide computer services, and to the staff of the Theresian Library, who gave us access to the Room and organized a display of the many historical books from their vast and precious collections which are of interest to physicists. Above all, the success of the meeting is due to the participants who animated it, and in particular to the speakers for their dedicated work in preparing their excellent talks and in providing the write-ups, and to the conveners for their essential role in shaping an interesting and well balanced scientific program. Finally, we wish to thank the International Advisory Committee for their unfailing support and for offering us the opportunity to organize this Conference in Pavia. Michele Livan Chairman, Organizing Committee International

  13. A Lunar Laser Ranging Retroreflector for the 21st Century

    NASA Astrophysics Data System (ADS)

    Currie, D.; Dell-Agnello, S.; Delle Monache, G.

    Over the past forty years, Lunar Laser Ranging (LLR) to the Apollo Cube Corner (CCR) Retroreflector arrays has supplied almost all of the significant tests of General Relativity. The LLR program has evaluated the PPN parameters and addressed, for example, the possible change in the gravitational constant and the properties of the self-energy of the gravitational field. In addition, LLR has provided significant information on the composition and origin of the moon. These arrays are the only experiment of the Apollo program that are still in operation. Initially the Apollo Lunar Arrays contributed a negligible portion of the error budget used to achieve these results. Over the decades, the performance of ground stations has greatly upgraded so that the ranging accuracy has improved by more than two orders of magnitude, i.e., a factor of 140. Now, after forty years, because of the lunar librations the existing Apollo retroreflector arrays contribute significant fraction of the limiting errors in the range measurements. The University of Maryland, as the Principal Investigator for the original Apollo arrays, is now proposing a new approach to the Lunar Laser CCR array technology. The investigation of this new technology, with Professor Currie as Principal Investigator, is currently being supported by two NASA programs and, in part, also by INFN/LNF. Thus after the proposed installation on the next Lunar landing, the new arrays will support ranging observations that are a factor 100 more accurate than the current Apollo LLRRAs, from the centimeter level to the micron level. The new fundamental physics and the lunar physics that this new LLRRA can provide will be described. In the design of the new array, there are three major challenges: 1) Validate that the specifications of the CCR required for the new array, with are significantly beyond the properties of current CCRs, can indeed be achieved. 2) Address the thermal and optical effects of the absorption of solar

  14. PREFACE: Fourth Meeting on Constrained Dynamics and Quantum Gravity

    NASA Astrophysics Data System (ADS)

    Cadoni, Mariano; Cavaglia, Marco; Nelson, Jeanette E.

    2006-04-01

    groups. In particular, help was provided to students and scientists from non-EU countries. It is our great pleasure to thank those people and institutions whose help and support was crucial to the success of the meeting. We appreciate the enthusiastic support of our colleagues of the academic community, especially those from the Istituto Nazionale di Fisica Nucleare and the Universities of Cagliari, Pisa, Torino and Mississippi. Financial support was provided by the Istituto Nazionale di Fisica Nucleare, the Universities of Cagliari, Torino, Pisa and Mississippi. This was used largely to support participants, especially younger people. Special thanks go to Pietro Menotti (University of Pisa) and Stefano Sciuto (University of Torino) for their friendship and their universities' financial contributions. It is also a pleasure to acknowledge financial support from the Regione Autonoma della Sardegna and from Tiscali, the communications and Internet company, for providing free telephone cards. Technical support and local organisation was provided by the Sezione di Cagliari of the Istituto Nazionale di Fisica Nucleare. Warmest thanks go to our administrative and technical staff - Maria Assunta Lecca and Simona Renieri, for their untiring assistance, and to Palmasera Village and Hotel Smeraldo for their splendid hospitality. And finally, our gratitude goes to all the participants and especially the many experienced scientists. Their contributions highlighted the meeting and were largely without support. The success of the meeting is due to them and to the enthusiasm of the younger participants. The Editors January 2006 COMMITTEES Organising Committee Mariano Cadoni (Università and INFN Cagliari) Marco Cavaglià (University of Mississippi) Jeanette E. Nelson (Università and INFN Torino) Advisory Committee Orfeu Bertolami (IST Lisboa, Portugal) Luca Bombelli (Univ. Mississippi) Steve Carlip (UC Davis, USA) Alessandro D'Adda (INFN Torino, Italy) Stanley Deser (Brandeis, USA

  15. PREFACE: First Mediterranean Conference on Classical and Quantum Gravity (MCCQG 2009)

    NASA Astrophysics Data System (ADS)

    Basilakos, Spyros; Cadoni, Mariano; Cavaglia, Marco; Christodoulakis, Theodosios; Vagenas, Elias C.

    2010-04-01

    quite fruitful, enjoyable 'Mediterranean' atmosphere for the exchange of ideas and discussion. It is a pleasure to thank our administrative and technical staff Georgia Angelopoulou, Athina Pouri, Mando Zambeli and Manolis Zoulias for their untiring assistance. We also thank the staff of the OAC for the enthusiastic support and their hospitality. We are grateful to the Academy of Athens and the Tomalla Foundation for their generous financial support which made MCCQG possible. Finally, our gratitude goes to all the participants and especially the many experienced scientists. Their contributions highlighted the meeting. The success of the MCCQG is due to them and to the enthusiasm of the younger participants. The Editors March 2010 COMMITTEES Organising Committee Spyros Basilakos (RCAAM, Academy of Athens, Greece) Mariano Cadoni (University and INFN Cagliari, Italy) Marco Cavaglià (University of Mississippi, USA) Theodosios Christodoulakis (University of Athens, Greece) Elias Vagenas (RCAAM, Academy of Athens, Greece) Advisory Committee Ignatios Antoniadis (CERN, Switzerland) Orfeu Bertolami (IST, Lisbon, Portugal) Loriano Bonora (SISSA, Trieste, Italy) George Contopoulos (Academy of Athens, Greece) Ruth Durrer (Geneva University, Switzerland) Enrique Gaztanaga (IEEC, Barcelona, Spain) Gabriela Gonzalez (Louisiana State University, Baton Rouge, USA) Marc Henneaux (Brussels University, Belgium) Roman Jackiw (MIT, USA) Claus Kiefer (Cologne University, Germany) Stefano Liberati (SISSA, Trieste, Italy) Ofer Lahav (University College London, UK) Roy Maartens (University of Portsmouth, UK) Don Marolf (UC Santa Barbara, USA) Hermann Nicolai (AEI, Potsdam, Germany) Augusto Sagnotti (Scuola Normale Superiore, Pisa, Italy) Mairi Sakellariadou (King's College London, UK) Jorge Zanelli (CECS, Valdivia, Chile) SPONSORS Academy of Athens The Tomalla Foundation Università di Cagliari University of Mississippi University of Athens LIST OF PARTICIPANTS Abdalla, Elcio (Instituto de

  16. Precision studies of the NNLO DGLAP evolution at the LHC with Candia

    NASA Astrophysics Data System (ADS)

    Cafarella, Alessandro; Corianò, Claudio; Guzzi, Marco

    2008-11-01

    We summarize the theoretical approach to the solution of the NNLO DGLAP equations using methods based on the logarithmic expansions in x-space and their implementation into the C program CANDIA 1.0. We present the various options implemented in the program and discuss the different solutions. The user can choose the order of the evolution, the type of the solution, which can be either exact or truncated, and the evolution either with a fixed or a varying flavor number, implemented in the varying-flavor-number scheme (VFNS). The renormalization and factorization scale dependencies are treated separately. In the non-singlet sector the program implements an exact NNLO solution. Program summaryProgram title: CANDIA Catalogue identifier: AEBK_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEBK_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 101 376 No. of bytes in distributed program, including test data, etc.: 5 865 234 Distribution format: tar.gz Programming language: C and Fortran Computer: All Operating system: Linux RAM: In the given examples, it ranges from 4 to 490 MB Classification: 11.1, 11.5 Nature of problem: The program provided here solves the DGLAP evolution equations for the parton distribution functions up to NNLO. Solution method: The algorithm implemented is based on the theory of the logarithmic expansions in Bjorken x-space. Additional comments: To be sure of getting the latest version of the program, the authors suggest downloading the code from their official CANDIA website ( http://www.le.infn.it/candia). Running time: In the given examples, it ranges from 1 to 40 minutes. The jobs have been executed on an Intel Core 2 Duo T7250 CPU at 2 GHz with a 64 bit Linux kernel. The test run script included in the package contains 5 sample runs and

  17. The Moon as a Test Body for General Relativity and New Gravitational Theories

    NASA Astrophysics Data System (ADS)

    Martini, Manuele; March, Riccardo; Bellettini, Giovanni; Dell'Agnello, S.; Delle Monache, G. O.; Currie, D. G.; Martini, M.; Lops, C.; Garattini, M.; March, R.; Bellettini, G.; Tauraso, R.; Battat, J. B.; Bianco, G.; Murphy, T. W., Jr.; Coradini, A.; Boni, A.; Cantone, C.; Maiello, M.; Porcelli, L.; Berardi, S.; Intaglietta, N.

    Since 1969 Lunar Laser Ranging (LLR) to the Apollo Cube Corner Reflector (CCR) arrays has supplied several significant tests of General Relativity (GR): it has evaluated the Geodetic Precession, probed the weak and strong equivalence principle, determined the PPN parameter , addressed the time change of G and 1/r2 deviations. We show that the Moon equipped with retroreflectors can be used effectively to test new gravitational theories beyond GR, like spacetime torsion (developed by some of the authors) and the unified braneworld theory by G. Dvali et al. LLR has also provided important information on the composition and origin of the Moon through measurement of its rotations and tides. Future robotic lunar missions, like the proposed International Lunar Network (ILN) will greatly expand this broad scientific program.Initially, the Apollo arrays contributed a negligible portion of the LLR error budget. Nowadays, the ranging accuracy of ground stations has improved by more than two orders of magnitude: the new APOLLO station at Apache Point, USA, is capable of mm-level range measurements; MRLO, at the ASI Space Geodesy Center in Matera, Italy, has re-started LR operations. Now, because of lunar librations, the Apollo arrays dominate the LLR error budget, which is a few cm. The University of Maryland, Principal Investigator for the Apollo arrays, and INFN-LNF are proposing an innovative CCR array design that will reduce the error contribution of LLR payloads by more than two orders of magnitude, down to tens of microns. This is the goal of the MoonLIGHT technological experiment of INFN (Moon Laser Instrumentation for General relativity High-Accuracy Tests) and of the SCF, the CCR space test facility at LNF. We have also proposed the precursor test of the MoonLIGHT payload on the ASI lunar orbiter mission MAGIA (A. Coradini PI), which concluded its Phase A Study in 2009. In our new array design the main challenges are: 1) address the thermal and optical effects of the

  18. Study of the production of and hadrons in pp collisions and first measurement of the branching fraction

    NASA Astrophysics Data System (ADS)

    Aaij, R.; Adeva, B.; Adinolfi, M.; Affolder, A.; Ajaltouni, Z.; Akar, S.; Albrecht, J.; Alessio, F.; Alexander, M.; Ali, S.; Alkhazov, G.; Alvarez Cartelle, P.; Alves, A. A., Jr.; Amato, S.; Amerio, S.; Amhis, Y.; An, L.; Anderlini, L.; Anderson, J.; Andreassi, G.; Andreotti, M.; Andrews, J. E.; Appleby, R. B.; Aquines Gutierrez, O.; Archilli, F.; d'Argent, P.; Artamonov, A.; Artuso, M.; Aslanides, E.; Auriemma, G.; Baalouch, M.; Bachmann, S.; Back, J. J.; Badalov, A.; Baesso, C.; Baldini, W.; Barlow; , R. J.Barschel, C.; Barsuk, S.; Barter, W.; Batozskaya, V.; Battista, V.; Bay, A.; Beaucourt, L.; Beddow, J.; Bedeschi, F.; Bediaga, I.; Bel, L. J.; Bellee, V.; Belloli, N.; Belyaev, I.; Ben-Haim, E.; Bencivenni, G.; Benson, S.; J., Benton; Berezhnoy, A.; Bernet, R.; Bertolin, A.; Bettler, M.-O.; van Beuzekom, M.; Bien, A.; Bifani, S.; Billoir, P.; Bird, T.; Birnkraut, A.; Bizzeti, A.; Blake, T.; Blanc, F.; Blouw, J.; Blusk, S.; Bocci, V.; Bondar, A.; Bondar, N.; Bonivento, W.; Borghi, S.; Borsato, M.; Bowcock, T. J. V.; Bowen, E.; Bozzi, C.; Braun, S.; Britsch, M.; Britton, T.; Brodzicka, J.; Brook, N. H.; Buchanan, E.; Bursche, A.; Buytaert, J.; Cadeddu, S.; Calabrese, R.; Calvi, M.; Calvo Gomez, M.; Campana, P.; Campora Perez, D.; Capriotti, L.; Carbone, A.; Carboni, G.; Cardinale, R.; Cardini, A.; Carniti, P.; Carson, L.; Carvalho, Akiba K.; Casse, G.; Cassina, L.; Castillo Garcia, L.; Cattaneo, M.; Cauet, Ch.; Cavallero, G.; Cenci, R.; Charles, M.; Charpentier, Ph.; Chefdeville, M.; Chen, S.; Cheung, S.-F.; Chiapolini, N.; Chrzaszcz, M.; Cid Vidal, X.; Ciezarek, G.; Clarke, P. E. L.; Clemencic, M.; Cliff, H. V.; Closier, J.; Coco, V.; Cogan, J.; Cogneras, E.; Cogoni, V.; Cojocariu, L.; Collazuol, G.; Collins, P.; Comerma-Montells, A.; Contu, A.; Cook, A.; Coombes, M.; Coquereau, S.; Corti, G.; Corvo, M.; Couturier, B.; Cowan, G. A.; Craik, D. C.; Crocombe, A.; Cruz Torres, M.; Cunliffe, S.; Currie, R.; D'Ambrosio, C.; Dall'Occo, E.; Dalseno, J.; David, P. N. Y.; Davis, A.; De Bruyn, K.; De Capua, S.; De Cian, M.; De Miranda, J. M.; De Paula, L.; De Simone, P.; Dean, C.-T.; Decamp, D.; Deckenhoff, M.; Del Buono, L.; Déléage, N.; Demmer, M.; Derkach, D.; Deschamps, O.; Dettori, F.; Dey, B.; Di Canto, A.; Di Ruscio, F.; Dijkstra, H.; Donleavy, S.; Dordei, F.; Dorigo, M.; Dosil Suárez, A.; Dossett, D.; Dovbnya, A.; Dreimanis, K.; Dufour, L.; Dujany, G.; Dupertuis, F.; Durante, P.; Dzhelyadin, R.; Dziurda, A.; Dzyuba, A.; Easo, S.; Egede, U.; Egorychev, V.; Eidelman, S.; Eisenhardt, S.; Eitschberger, U.; Ekelhof, R.; Eklund, L.; El Rifai, I.; Elsasser, Ch.; Ely, S.; Esen, S.; Evans, H. M.; Evans, T.; Falabella, A.; Färber, C.; Farley, N.; Farry, S.; Fay, R.; Ferguson, D.; Fernandez Albor, V.; Ferrari, F.; Ferreira Rodrigues, F.; Ferro-Luzzi, M.; Filippov, S.; Fiore, M.; Fiorini, M.; Firlej, M.; Fitzpatrick, C.; Fiutowski, T.; Fohl, K.; Fol, P.; Fontana, M.; Fontanelli, F.; Forty, R.; Francisco, O.; Frank, M.; Frei, C.; Frosini, M.; Fu, J.; Furfaro, E.; Gallas, A., Torreira; Galli, D.; Gallorini, S.; Gambetta, S.; Gandelman, M.; Gandini, P.; Gao, Y.; García Pardiñas, J.; Garra Tico, J.; Garrido, L.; Gascon, D.; Gaspar, C.; Gauld, R.; Gavardi, L.; Gazzoni, G.; Gerick, D.; Gersabeck, E.; Gersabeck, M.; Gershon, T.; Ghez, Ph.; Gianì, S.; Gibson, V.; Girard, O. G.; Giubega, L.; Gligorov, V. V.; Göbel, C.; Golubkov, D.; Golutvin, A.; Gomes, A.; Gotti, C.; Grabalosa Gándara, M.; Graciani Diaz, R.; Granado Cardoso, L. A.; Graugés, E.; Graverini, E.; Graziani, G.; Grecu, A.; Greening, E.; Gregson, S.; Griffith, P.; Grillo, L.; Grünberg, O.; Gui, B.; Gushchin, E.; Guz, Yu.; Gys, T.; Hadavizadeh, T.; Hadjivasiliou, C.; Haefeli, G.; Haen, C.; Haines, S. C.; Hall, S.; Hamilton, B.; Han, X.; Hansmann-Menzemer, S.; Harnew, N.; Harnew, S. T.; Harrison, J.; He, J.; Head, T.; Heijne, V.; Hennessy, K.; Henrard, P.; Henry, L.; van Herwijnen, E.; Heß, M.; Hicheur, A.; Hill, D.; Hoballah, M.; Hombach, C.; Hulsbergen, W.; Humair, T.; Hussain, N.; Hutchcroft, D.; Hynds, D.; Idzik, M.; Ilten, P.; Jacobsson, R.; Jaeger, A.; Jalocha, J.; Jans, E.; Jawahery, A.; Jing; M. F., John; Johnson, D.; Jones, C. F.; Joram, C.; Jost, B.; Jurik, N.; Kandybei, S.; Kanso, W.; Karacson, M.; Karbach, T. M.; Karodia, S.; Kecke, M.; Kelsey, M.; Kenyon, I. R.; Kenzie, M.; Ketel, T.; Khanji, B.; Khurewathanakul, C.; Klaver, S.; Klimaszewski, K.; Kochebina, O.; Kolpin, M.; Komarov, I.; Koopman, R. F.; Koppenburg, P.; Kozeiha, M.; Kravchuk, L.; Kreplin, K.; Kreps, M.; Krocker, G.; Krokovny, P.; Kruse, F.; Krzemien, W.; Kucewicz, W.; Kucharczyk, M.; Kudryavtsev, V.; Kuonen, A. K.; Kurek, K.; Kvaratskheliya, T.; Lacarrere, D.; Lafferty, G.; Lai, A.; Lambert, D.; Lanfranchi, G.; Langenbruch, C.; Langhans, B.; Latham, T.; Lazzeroni, C.; Le Gac, R.

    2016-01-01

    The product of the differential production cross-section and the branching fraction of the decay is measured as a function of the beauty hadron transverse momentum, pT, and rapidity, y. The kinematic region of the measurements is pT < 20 GeV/c and 2.0 < y < 4.5. The measurements use a data sample corresponding to an integrated luminosity of 3fb-1 collected by the LHCb detector in pp collisions at centre-of-mass energies in 2011 and in 2012. Based on previous LHCb results of the fragmentation fraction ratio the branching fraction of the decay is measured to be where the first uncertainty is statistical, the second is systematic, the third is due to the uncertainty on the branching fraction of the decay B̅0 → J/ψK̅*(892)0, and the fourth is due to the knowledge of . The sum of the asymmetries in the production and decay between and is also measured as a function of pT and y. The previously published branching fraction of , relative to that of , is updated. The branching fractions of are determined. Supported by CERN and national agencies: CAPES, CNPq, FAPERJ and FINEP (Brazil); NSFC (China); CNRS/IN2P3 (France); BMBF, DFG, HGF and MPG (Germany); INFN (Italy); FOM and NWO (The Netherlands); MNiSW and NCN (Poland); MEN/IFA (Romania); MinES and FANO (Russia); MinECo (Spain); SNSF and SER (Switzerland); NASU (Ukraine); STFC (United Kingdom); NSF (USA). The Tier1 computing centres are supported by IN2P3 (France), KIT and BMBF (Germany), INFN (Italy), NWO and SURF (The Netherlands), PIC (Spain), GridPP (United Kingdom). Individual groups or members have received support from EPLANET, Marie Skłodowska-Curie Actions and ERC (European Union), Conseil général de Haute-Savoie, Labex ENIGMASS and OCEVU, Région Auvergne (France), RFBR (Russia), XuntaGal and GENCAT (Spain), Royal Society and Royal Commission for the Exhibition of 1851 (United Kingdom).

  19. Background noise levels and correlation with ship traffic in the Gulf of Catania

    NASA Astrophysics Data System (ADS)

    Viola, Salvatore; Buscaino, Giuseppa; Caruso, Francesco; Chierici, Francesco; Embriaco, Davide; Favali, Paolo; Giovanetti, Gabriele; Grammauta, Roasario; Larosa, Giuseppina; Pavan, Gianni; Pellegrino, Carmelo; Pulvirenti, Sara; Riccobene, Giorgio; Sciacca, Virginia; Simeone, Francesco; Beranzoli, Laura; Marinaro, Giuditta

    2015-04-01

    In the last decades the growing interest in the evaluation of the underwater acoustic noise for studies in the fields of geology, biology and high-energy physics is driving the scientific community to collaborate towards a multidisciplinary approach to the topic. In June 2012 in the framework of the European project EMSO, a multidisciplinary underwater observatory, named NEMO-SN1, was installed 25 km off-shore the port of Catania, at a depth of 2100 m and operated until May 2013 by INFN (Istituto Nazionale di Fisica Nucleare) and INGV (Istituto Nazionale di Geofisica e Vulcanologia). NEMO-SN1 hosted aboard geophysical, oceanographic and acoustic sensors: among these a seismic hydrophone model SMID DT-405D(V). In this work, conducted within the activity of the SMO project, the results on the evaluation of the underwater acoustic pollution in the Gulf of Catania through SMID DT-405D(V) recordings are presented. The seismic hydrophone provided a data set of about 11 months of continuous (24/7) recordings. Underwater sounds have been continuously digitized at a sampling frequency of 2 kHz and the acquired data have been stored in 10min long files for off-line analysis. To describe one-year background noise levels, the mean integrated acoustic noise was measured every second (sampling frequency 2000, NFFT 2048) in the 1/3 octave bands with centre frequency 63 Hz and for each 10 minutes-long file the 5th, the 50th and the 98th percentiles were calculated. Measured noise was correlated with the shipping traffic in the area, thanks to the data provided by an AIS receiver installed at the INFN-Laboratori Nazionali del Sud. An acoustic noise increment was measured in coincidence with the passing of crafts in the area and it was possible to identify the characteristic spectrum of each ship. A simple model for the estimation of the acoustic noise induced by the ships passing through the area was developed. The model was applied by using AIS data acquired during the operation

  20. The Legnaro-Padova distributed Tier-2: challenges and results

    NASA Astrophysics Data System (ADS)

    Badoer, Simone; Biasotto, Massimo; Costa, Fulvia; Crescente, Alberto; Fantinel, Sergio; Ferrari, Roberto; Gulmini, Michele; Maron, Gaetano; Michelotto, Michele; Sgaravatto, Massimo; Toniolo, Nicola

    2014-06-01

    The Legnaro-Padova Tier-2 is a computing facility serving the ALICE and CMS LHC experiments. It also supports other High Energy Physics experiments and other virtual organizations of different disciplines, which can opportunistically harness idle resources if available. The unique characteristic of this Tier-2 is its topology: the computational resources are spread in two different sites, about 15 km apart: the INFN Legnaro National Laboratories and the INFN Padova unit, connected through a 10 Gbps network link (it will be soon updated to 20 Gbps). Nevertheless these resources are seamlessly integrated and are exposed as a single computing facility. Despite this intrinsic complexity, the Legnaro-Padova Tier-2 ranks among the best Grid sites for what concerns reliability and availability. The Tier-2 comprises about 190 worker nodes, providing about 26000 HS06 in total. Such computing nodes are managed by the LSF local resource management system, and are accessible using a Grid-based interface implemented through multiple CREAM CE front-ends. dCache, xrootd and Lustre are the storage systems in use at the Tier-2: about 1.5 PB of disk space is available to users in total, through multiple access protocols. A 10 Gbps network link, planned to be doubled in the next months, connects the Tier-2 to WAN. This link is used for the LHC Open Network Environment (LHCONE) and for other general purpose traffic. In this paper we discuss about the experiences at the Legnaro-Padova Tier-2: the problems that had to be addressed, the lessons learned, the implementation choices. We also present the tools used for the daily management operations. These include DOCET, a Java-based webtool designed, implemented and maintained at the Legnaro-Padova Tier-2, and deployed also in other sites, such as the LHC Italian T1. DOCET provides an uniform interface to manage all the information about the physical resources of a computing center. It is also used as documentation repository available to