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Sample records for allison gt 404-4

  1. 48 CFR 1615.404-4 - Profit.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Profit. 1615.404-4 Section 1615.404-4 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT FEDERAL EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY...

  2. 48 CFR 315.404-4 - Profit.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... section. The Contracting Officer's analysis of these factors shall be based on available information, such... the profit analysis factors in FAR 15.404-4(d) in lieu of the structured approach in the following... operations or maintenance of Government facilities. (D) Construction contracts. (E) Contracts...

  3. 48 CFR 915.404-4-71-2 - Limitations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Limitations. 915.404-4-71-2 Section 915.404-4-71-2 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-2 Limitations....

  4. 48 CFR 915.404-4-70-7 - Alternative techniques.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Alternative techniques. 915.404-4-70-7 Section 915.404-4-70-7 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-7...

  5. 48 CFR 915.404-4-70-7 - Alternative techniques.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Alternative techniques. 915.404-4-70-7 Section 915.404-4-70-7 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-7...

  6. 48 CFR 915.404-4-71-2 - Limitations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Limitations. 915.404-4-71-2 Section 915.404-4-71-2 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-2 Limitations....

  7. 48 CFR 915.404-4-71-2 - Limitations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Limitations. 915.404-4-71-2 Section 915.404-4-71-2 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-2 Limitations....

  8. 48 CFR 915.404-4-70-3 - Documentation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Documentation. 915.404-4-70-3 Section 915.404-4-70-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-3...

  9. 48 CFR 915.404-4-70-3 - Documentation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Documentation. 915.404-4-70-3 Section 915.404-4-70-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-3...

  10. 48 CFR 915.404-4-71-2 - Limitations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Limitations. 915.404-4-71-2 Section 915.404-4-71-2 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-2 Limitations....

  11. 48 CFR 915.404-4-70-7 - Alternative techniques.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Alternative techniques. 915.404-4-70-7 Section 915.404-4-70-7 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-7...

  12. 48 CFR 915.404-4-71-2 - Limitations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Limitations. 915.404-4-71-2 Section 915.404-4-71-2 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-2 Limitations....

  13. 48 CFR 915.404-4-70-7 - Alternative techniques.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Alternative techniques. 915.404-4-70-7 Section 915.404-4-70-7 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-7...

  14. 48 CFR 915.404-4-70-3 - Documentation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Documentation. 915.404-4-70-3 Section 915.404-4-70-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-3...

  15. 48 CFR 915.404-4-70-3 - Documentation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Documentation. 915.404-4-70-3 Section 915.404-4-70-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-3...

  16. 48 CFR 915.404-4-70-3 - Documentation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Documentation. 915.404-4-70-3 Section 915.404-4-70-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-3...

  17. 48 CFR 915.404-4-70-7 - Alternative techniques.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Alternative techniques. 915.404-4-70-7 Section 915.404-4-70-7 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-7...

  18. Overview of Allison/AGTSR interactions

    SciTech Connect

    Ali, S.A.

    1995-10-01

    Morgantown Energy Technology Center`s Tom Bechtel`s perseverance resulted in the creation of the Advanced Gas Turbine Systems Research (AGTSR) initiative in 1992. His foresight resulted in establishing an advanced gas turbine research center. The center is supported by the Government and industry. The industry serves in an oversight role, and helps select the type of research projects to be conducted by universities. AGTSR now represents an effective university and industry partnership, and is recognized as a positive mechanism. Allison Engine Company (Allison) continues to be an active participant and a strong supporter of the Advanced Gas Turbine Systems Research initiative at Clemson University. Allison took part in early formulation stages of this university/industry consortium. We have been a member of the consortium since its inception in 1992. Allison`s senior management is cognizant of the benefits of these jointly sponsored research programs. Over the years, Allison has promoted cooperative gas turbine research and advanced technology development at many universities. This activity is conducted through direct assignments at Allison for university students, and support of research projects at various universities, focused on different disciplines of gas turbine research.

  19. 50 CFR 404.4 - Access to Monument.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Access to Monument. 404.4 Section 404.4 Wildlife and Fisheries JOINT REGULATIONS (UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR AND NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION,...

  20. 50 CFR 404.4 - Access to Monument.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Access to Monument. 404.4 Section 404.4 Wildlife and Fisheries JOINT REGULATIONS (UNITED STATES FISH AND WILDLIFE SERVICE, DEPARTMENT OF THE INTERIOR AND NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION,...

  1. 48 CFR 915.404-4-71-5 - Fee schedules.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...-71-5 Section 915.404-4-71-5 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-5 Fee schedules. (a... maximum fee amount for a CPFF contract arrangement. If a fixed-price type contract is to be awarded,...

  2. 48 CFR 915.404-4-71-6 - Fee base.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Section 915.404-4-71-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-6 Fee base. (a) The fee base shown... appropriate to the type of contract; except, any home office G&A expense paid as a contract cost per...

  3. 48 CFR 915.404-4-70-4 - Exceptions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Section 915.404-4-70-4 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-4 Exceptions. (a) For contracts not... used— (1) Commercialization and demonstration type contracts; (2) Management and operating...

  4. 48 CFR 915.404-4-71-6 - Fee base.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Section 915.404-4-71-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-6 Fee base. (a) The fee base shown... appropriate to the type of contract; except, any home office G&A expense paid as a contract cost per...

  5. 48 CFR 915.404-4-70-1 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Section 915.404-4-70-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-1 General. (a) Objective. It is the... type of work involved. The profit objective should be reasonable in relation to the task to...

  6. 48 CFR 915.404-4-70-4 - Exceptions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Section 915.404-4-70-4 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-4 Exceptions. (a) For contracts not... used— (1) Commercialization and demonstration type contracts; (2) Management and operating...

  7. 48 CFR 915.404-4-71-5 - Fee schedules.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...-71-5 Section 915.404-4-71-5 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-5 Fee schedules. (a... maximum fee amount for a CPFF contract arrangement. If a fixed-price type contract is to be awarded,...

  8. 48 CFR 915.404-4-71-1 - General.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Section 915.404-4-71-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-1 General. (a) Business concerns... shall be an amount appropriate for the type of effort contained therein. It is the intent of DOE to—...

  9. 48 CFR 915.404-4-71-6 - Fee base.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Section 915.404-4-71-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-6 Fee base. (a) The fee base shown... appropriate to the type of contract; except, any home office G&A expense paid as a contract cost per...

  10. 48 CFR 915.404-4-70-4 - Exceptions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Section 915.404-4-70-4 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-4 Exceptions. (a) For contracts not... used— (1) Commercialization and demonstration type contracts; (2) Management and operating...

  11. 48 CFR 915.404-4-70-4 - Exceptions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Section 915.404-4-70-4 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-4 Exceptions. (a) For contracts not... demonstration type contracts; (2) Management and operating contracts; (3) Construction contracts;...

  12. 48 CFR 915.404-4-71-6 - Fee base.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Section 915.404-4-71-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-6 Fee base. (a) The fee base shown... appropriate to the type of contract; except, any home office G&A expense paid as a contract cost per...

  13. 48 CFR 915.404-4-71-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Section 915.404-4-71-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-1 General. (a) Business concerns... shall be an amount appropriate for the type of effort contained therein. It is the intent of DOE to...

  14. 48 CFR 915.404-4-70-1 - General.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Section 915.404-4-70-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-1 General. (a) Objective. It is the... type of work involved. The profit objective should be reasonable in relation to the task to...

  15. 48 CFR 915.404-4-71-1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Section 915.404-4-71-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-1 General. (a) Business concerns... shall be an amount appropriate for the type of effort contained therein. It is the intent of DOE to—...

  16. 48 CFR 915.404-4-71-1 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Section 915.404-4-71-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-1 General. (a) Business concerns... shall be an amount appropriate for the type of effort contained therein. It is the intent of DOE to—...

  17. 48 CFR 915.404-4-71-6 - Fee base.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Section 915.404-4-71-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-6 Fee base. (a) The fee base shown... appropriate to the type of contract; except, any home office G&A expense paid as a contract cost per...

  18. 48 CFR 915.404-4-70-1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Section 915.404-4-70-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-1 General. (a) Objective. It is the... type of work involved. The profit objective should be reasonable in relation to the task to...

  19. 48 CFR 915.404-4-71-5 - Fee schedules.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-71-5 Section 915.404-4-71-5 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-5 Fee schedules. (a... maximum fee amount for a CPFF contract arrangement. If a fixed-price type contract is to be awarded,...

  20. 48 CFR 915.404-4-71-1 - General.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Section 915.404-4-71-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-1 General. (a) Business concerns... shall be an amount appropriate for the type of effort contained therein. It is the intent of DOE to—...

  1. 48 CFR 915.404-4-70-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Section 915.404-4-70-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-1 General. (a) Objective. It is the... type of work involved. The profit objective should be reasonable in relation to the task to...

  2. 48 CFR 915.404-4-71-5 - Fee schedules.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...-71-5 Section 915.404-4-71-5 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-5 Fee schedules. (a... maximum fee amount for a CPFF contract arrangement. If a fixed-price type contract is to be awarded,...

  3. 48 CFR 915.404-4-70-4 - Exceptions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Section 915.404-4-70-4 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-4 Exceptions. (a) For contracts not... used— (1) Commercialization and demonstration type contracts; (2) Management and operating...

  4. 48 CFR 915.404-4-70-1 - General.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Section 915.404-4-70-1 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-1 General. (a) Objective. It is the... type of work involved. The profit objective should be reasonable in relation to the task to...

  5. Allison Sloan: Paraprofessional of the Year 2010

    ERIC Educational Resources Information Center

    Berry, John N., III

    2010-01-01

    This article profiles Allison Sloan, the 2010 winner of "LJ"'s Paraprofessional of the Year Award, sponsored by DEMCO, Inc. Her outstanding service and her championship of the term paralibrarian illustrates her passionately held and most fundamental belief: "This is not just a nice job, this is a career." When she launched that…

  6. Unified Process Planning, the Allison approach

    NASA Astrophysics Data System (ADS)

    Simmons, Clair

    Process Planning, the organization of product/process information for the manufacturing enterprise, represents the lead activity within the manufacturing portion of a business. Process planning encompasses all aspects that contribute to the successful manufacture of a quality product. This paper explains the role of process planning and how a 'Unified Process Plan' (UPP) provides Allison with a more effective and organized method of manufacturing information management. This approach allows for a more effective use of the manufacturing engineering staff. Allison is transitioning to a knowledge base of process information that will be continually improved and accessible to every engineer and operator. Unified Process Planning focuses key pieces of information into one manageable and controlled environment.

  7. 36 CFR 404.4 - Access to information.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... GUIDELINES FOR COMPLIANCE WITH THE FREEDOM OF INFORMATION ACT § 404.4 Access to information. (a) The American... file a request under the Freedom of Information Act (FOIA) should address their request in writing to..., certified to be true and correct to the best of that person's knowledge and belief, explaining in detail...

  8. 36 CFR 404.4 - Access to information.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... GUIDELINES FOR COMPLIANCE WITH THE FREEDOM OF INFORMATION ACT § 404.4 Access to information. (a) The American... file a request under the Freedom of Information Act (FOIA) should address their request in writing to..., certified to be true and correct to the best of that person's knowledge and belief, explaining in detail...

  9. 48 CFR 915.404-4-70-8 - Weighted guidelines application considerations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Weighted guidelines application considerations. 915.404-4-70-8 Section 915.404-4-70-8 Federal Acquisition Regulations System....404-4-70-8 Weighted guidelines application considerations. The Department has developed...

  10. 48 CFR 915.404-4-70-2 - Weighted guidelines system.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    .... 915.404-4-70-2 Section 915.404-4-70-2 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-2 Weighted... Risk (type of contract-weights applied to total cost of items 4.a. thru 4.e.) 0 to 8. III....

  11. 48 CFR 915.404-4-71-3 - Factors for determining fees.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... fees. 915.404-4-71-3 Section 915.404-4-71-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-3 Factors for... requirements; (2) The presence or absence of financial risk, including the type and terms of the contract;...

  12. 48 CFR 915.404-4-70-6 - Contracts with educational institutions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Contracts with educational institutions. 915.404-4-70-6 Section 915.404-4-70-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-6...

  13. 48 CFR 915.404-4-70-2 - Weighted guidelines system.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... 915.404-4-70-2 Section 915.404-4-70-2 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-2 Weighted... Risk (type of contract-weights applied to total cost of items 4.a. thru 4.e.) 0 to 8. III....

  14. 48 CFR 915.404-4-70-2 - Weighted guidelines system.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    .... 915.404-4-70-2 Section 915.404-4-70-2 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-2 Weighted... Risk (type of contract-weights applied to total cost of items 4.a. thru 4.e.) 0 to 8. III....

  15. 48 CFR 915.404-4-70-6 - Contracts with educational institutions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Contracts with educational institutions. 915.404-4-70-6 Section 915.404-4-70-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-6...

  16. 48 CFR 915.404-4-70-6 - Contracts with educational institutions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Contracts with educational institutions. 915.404-4-70-6 Section 915.404-4-70-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-6...

  17. 48 CFR 915.404-4-70-6 - Contracts with educational institutions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contracts with educational institutions. 915.404-4-70-6 Section 915.404-4-70-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-6...

  18. 48 CFR 915.404-4-71-3 - Factors for determining fees.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... fees. 915.404-4-71-3 Section 915.404-4-71-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-3 Factors for... requirements; (2) The presence or absence of financial risk, including the type and terms of the contract;...

  19. 48 CFR 915.404-4-71-3 - Factors for determining fees.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... fees. 915.404-4-71-3 Section 915.404-4-71-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-3 Factors for... requirements; (2) The presence or absence of financial risk, including the type and terms of the contract;...

  20. 48 CFR 915.404-4-71-3 - Factors for determining fees.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... fees. 915.404-4-71-3 Section 915.404-4-71-3 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-71-3 Factors for... requirements; (2) The presence or absence of financial risk, including the type and terms of the contract;...

  1. 48 CFR 915.404-4-70-2 - Weighted guidelines system.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... 915.404-4-70-2 Section 915.404-4-70-2 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-2 Weighted... Risk (type of contract-weights applied to total cost of items 4.a. thru 4.e.) 0 to 8. III....

  2. 48 CFR 915.404-4-70-6 - Contracts with educational institutions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Contracts with educational institutions. 915.404-4-70-6 Section 915.404-4-70-6 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-6...

  3. 48 CFR 14.404-4 - Restrictions on disclosure of descriptive literature.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of descriptive literature. 14.404-4 Section 14.404-4 Federal Acquisition Regulations System FEDERAL... Contract 14.404-4 Restrictions on disclosure of descriptive literature. When a bid is accompanied by descriptive literature (as defined in 2.101), and the bidder imposes a restriction that prevents the...

  4. 48 CFR 27.404-4 - Contractor's release, publication, and use of data.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Contractor's release, publication, and use of data. 27.404-4 Section 27.404-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS PATENTS, DATA, AND COPYRIGHTS Rights in Data...

  5. 48 CFR 1327.404-4 - Contractor's release, publication, and use of data.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Contractor's release, publication, and use of data. 1327.404-4 Section 1327.404-4 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE GENERAL CONTRACTING REQUIREMENTS PATENTS, DATA, AND COPYRIGHTS Rights in Data...

  6. 48 CFR 27.404-4 - Contractor's release, publication, and use of data.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Contractor's release, publication, and use of data. 27.404-4 Section 27.404-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS PATENTS, DATA, AND COPYRIGHTS Rights in Data...

  7. 48 CFR 1327.404-4 - Contractor's release, publication, and use of data.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contractor's release, publication, and use of data. 1327.404-4 Section 1327.404-4 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE GENERAL CONTRACTING REQUIREMENTS PATENTS, DATA, AND COPYRIGHTS Rights in Data...

  8. 48 CFR 915.404-4-72 - Special considerations for cost-plus-award-fee contracts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Special considerations for cost-plus-award-fee contracts. 915.404-4-72 Section 915.404-4-72 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  9. 48 CFR 915.404-4-70 - DOE structured profit and fee system.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false DOE structured profit and fee system. 915.404-4-70 Section 915.404-4-70 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  10. 48 CFR 915.404-4-70-8 - Weighted guidelines application considerations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Weighted guidelines application considerations. 915.404-4-70-8 Section 915.404-4-70-8 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  11. 48 CFR 915.404-4-70 - DOE structured profit and fee system.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false DOE structured profit and fee system. 915.404-4-70 Section 915.404-4-70 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  12. 48 CFR 915.404-4-72 - Special considerations for cost-plus-award-fee contracts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Special considerations for cost-plus-award-fee contracts. 915.404-4-72 Section 915.404-4-72 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  13. 48 CFR 915.404-4-72 - Special considerations for cost-plus-award-fee contracts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Special considerations for cost-plus-award-fee contracts. 915.404-4-72 Section 915.404-4-72 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  14. 48 CFR 915.404-4-70 - DOE structured profit and fee system.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false DOE structured profit and fee system. 915.404-4-70 Section 915.404-4-70 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  15. 48 CFR 915.404-4-70-8 - Weighted guidelines application considerations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Weighted guidelines application considerations. 915.404-4-70-8 Section 915.404-4-70-8 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  16. 48 CFR 915.404-4-72 - Special considerations for cost-plus-award-fee contracts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Special considerations for cost-plus-award-fee contracts. 915.404-4-72 Section 915.404-4-72 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  17. 48 CFR 915.404-4-70 - DOE structured profit and fee system.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false DOE structured profit and fee system. 915.404-4-70 Section 915.404-4-70 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  18. 48 CFR 915.404-4-70 - DOE structured profit and fee system.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false DOE structured profit and fee system. 915.404-4-70 Section 915.404-4-70 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  19. 48 CFR 915.404-4-70-8 - Weighted guidelines application considerations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Weighted guidelines application considerations. 915.404-4-70-8 Section 915.404-4-70-8 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  20. 48 CFR 915.404-4-70-8 - Weighted guidelines application considerations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Weighted guidelines application considerations. 915.404-4-70-8 Section 915.404-4-70-8 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing...

  1. 43 CFR 404.4 - What are the goals of the program?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false What are the goals of the program? 404.4..., DEPARTMENT OF THE INTERIOR RECLAMATION RURAL WATER SUPPLY PROGRAM Overview § 404.4 What are the goals of the program? The goals of the program are to: (a) Assess and address urgent and compelling rural water...

  2. 48 CFR 915.404-4-70-2 - Weighted guidelines system.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... circumstances, weightings have been developed for application by the contracting officer to standard measurement bases representative of the prescribed profit factors cited in FAR 15.404-4(d) and paragraph (d) of...

  3. Characterisation of the PXIE Allison-type emittance scanner

    SciTech Connect

    D'Arcy, R.; Alvarez, M.; Gaynier, J.; Prost, L.; Scarpine, V.; Shemyakin, A.

    2016-01-26

    An Allison-type emittance scanner has been designed for PXIE at FNAL with the goal of providing fast and accurate phase space reconstruction. The device has been modified from previous LBNL/SNS designs to operate in both pulsed and DC modes with the addition of water-cooled front slits. Extensive calibration techniques and error analysis allowed confinement of uncertainty to the <5% level (with known caveats). With a 16-bit, 1 MHz electronics scheme the device is able to analyse a pulse with a resolution of 1 μs, allowing for analysis of neutralisation effects. As a result, this paper describes a detailed breakdown of the R&D, as well as post-run analysis techniques.

  4. Characterisation of the PXIE Allison-type emittance scanner

    DOE PAGES

    D'Arcy, R.; Alvarez, M.; Gaynier, J.; ...

    2016-01-26

    An Allison-type emittance scanner has been designed for PXIE at FNAL with the goal of providing fast and accurate phase space reconstruction. The device has been modified from previous LBNL/SNS designs to operate in both pulsed and DC modes with the addition of water-cooled front slits. Extensive calibration techniques and error analysis allowed confinement of uncertainty to the <5% level (with known caveats). With a 16-bit, 1 MHz electronics scheme the device is able to analyse a pulse with a resolution of 1 μs, allowing for analysis of neutralisation effects. As a result, this paper describes a detailed breakdown ofmore » the R&D, as well as post-run analysis techniques.« less

  5. 43 CFR 404.4 - What are the goals of the program?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., water districts and associations, and other eligible entities, to address rural water supply issues; and (e) Complement other existing programs and authorities that address rural water supply issues, and..., DEPARTMENT OF THE INTERIOR RECLAMATION RURAL WATER SUPPLY PROGRAM Overview § 404.4 What are the goals of...

  6. 43 CFR 404.4 - What are the goals of the program?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., water districts and associations, and other eligible entities, to address rural water supply issues; and (e) Complement other existing programs and authorities that address rural water supply issues, and..., DEPARTMENT OF THE INTERIOR RECLAMATION RURAL WATER SUPPLY PROGRAM Overview § 404.4 What are the goals of...

  7. 43 CFR 404.4 - What are the goals of the program?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., water districts and associations, and other eligible entities, to address rural water supply issues; and (e) Complement other existing programs and authorities that address rural water supply issues, and..., DEPARTMENT OF THE INTERIOR RECLAMATION RURAL WATER SUPPLY PROGRAM Overview § 404.4 What are the goals of...

  8. 48 CFR 915.404-4-71 - Profit and fee-system for construction and construction management contracts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Profit and fee-system for construction and construction management contracts. 915.404-4-71 Section 915.404-4-71 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY...

  9. 48 CFR 915.404-4-71 - Profit and fee-system for construction and construction management contracts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Profit and fee-system for construction and construction management contracts. 915.404-4-71 Section 915.404-4-71 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY...

  10. 48 CFR 915.404-4-71 - Profit and fee-system for construction and construction management contracts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Profit and fee-system for construction and construction management contracts. 915.404-4-71 Section 915.404-4-71 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY...

  11. 48 CFR 915.404-4-71 - Profit and fee-system for construction and construction management contracts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 5 2012-10-01 2012-10-01 false Profit and fee-system for construction and construction management contracts. 915.404-4-71 Section 915.404-4-71 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY...

  12. 48 CFR 915.404-4-71 - Profit and fee-system for construction and construction management contracts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Profit and fee-system for construction and construction management contracts. 915.404-4-71 Section 915.404-4-71 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY...

  13. Advanced controls for airbreathing engines, volume 3: Allison gas turbine

    NASA Technical Reports Server (NTRS)

    Bough, R. M.

    1993-01-01

    The application of advanced control concepts to airbreathing engines may yield significant improvements in aircraft/engine performance and operability. Screening studies of advanced control concepts for airbreathing engines were conducted by three major domestic aircraft engine manufacturers to determine the potential impact of concepts on turbine engine performance and operability. The purpose of the studies was to identify concepts which offered high potential yet may incur high research and development risk. A target suite of proposed advanced control concepts was formulated and evaluated in a two-phase study to quantify each concept's impact on desired engine characteristics. To aid in the evaluation specific aircraft/engine combinations were considered: a Military High Performance Fighter mission, a High Speed Civil Transport mission, and a Civil Tiltrotor mission. Each of the advanced control concepts considered in the study are defined and described. The concept potential impact on engine performance was determined. Relevant figures of merit on which to evaluate the concepts are determined. Finally, the concepts are ranked with respect to the target aircraft/engine missions. A final report describing the screening studies was prepared by each engine manufacturer. Volume 3 of these reports describes the studies performed by the Allison Gas Turbine Division.

  14. 48 CFR 915.404-4-70-5 - Special considerations-contracts with nonprofit organizations (other than educational institutions).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...-4-70-5 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-5 Special considerations—contracts...

  15. 48 CFR 915.404-4-70-5 - Special considerations-contracts with nonprofit organizations (other than educational institutions).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...-4-70-5 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-5 Special considerations—contracts...

  16. 48 CFR 915.404-4-70-5 - Special considerations-contracts with nonprofit organizations (other than educational institutions).

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...-4-70-5 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-5 Special considerations—contracts...

  17. Rice Glycosyltransferase (GT) Phylogenomic Database

    DOE Data Explorer

    Ronald, Pamela

    The Ronald Laboratory staff at the University of California-Davis has a primary research focus on the genes of the rice plant. They study the role that genetics plays in the way rice plants respond to their environment. They created the Rice GT Database in order to integrate functional genomic information for putative rice Glycosyltransferases (GTs). This database contains information on nearly 800 putative rice GTs (gene models) identified by sequence similarity searches based on the Carbohydrate Active enZymes (CAZy) database. The Rice GT Database provides a platform to display user-selected functional genomic data on a phylogenetic tree. This includes sequence information, mutant line information, expression data, etc. An interactive chromosomal map shows the position of all rice GTs, and links to rice annotation databases are included. The format is intended to "facilitate the comparison of closely related GTs within different families, as well as perform global comparisons between sets of related families." [From http://ricephylogenomics.ucdavis.edu/cellwalls/gt/genInfo.shtml] See also the primary paper discussing this work: Peijian Cao, Laura E. Bartley, Ki-Hong Jung and Pamela C. Ronalda. Construction of a Rice Glycosyltransferase Phylogenomic Database and Identification of Rice-Diverged Glycosyltransferases. Molecular Plant, 2008, 1(5): 858-877.

  18. Asymmetric GT of social networks

    NASA Astrophysics Data System (ADS)

    Szu, Harold

    2010-04-01

    Web citation indexes are computed according to a data vector X collected from the frequency of user accesses, citations weighted by other sites' popularities, and modified by the financial sponsorship in a proprietary manner. The indexing determining the information to be retrieved by the public should be made responsible transparently in at least two ways. One shall balance the inbound linkages pointed at the specific i-th site called the popularity (see paper for equation) with the outbound linkages (see paper for equation) called the risk factor before the release of new information as environmental impact analysis. The relationship between these two factors cannot be assumed equivalent (undirected) as in the case of many mainstream Graph Theory (GT) models.

  19. Registration of maize inbred line GT603

    Technology Transfer Automated Retrieval System (TEKTRAN)

    GT603 (Reg. No. xxxx, PI xxxxxx) is a yellow dent maize (Zea mays L.) inbred line developed and released by the USDA-ARS Crop Protection and Management Research Unit in cooperation with the University of Georgia Coastal Plain Experiment Station in 2010. GT603 was developed through seven generations ...

  20. Registration of maize inbred line 'GT888'

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Maize (Zea mays L.) inbred line GT888 (PI 670116) was developed and released by the USDA-ARS in cooperation with the University of Georgia, and in participation with the USDA Germplasm Enhancement of Maize (GEM) project. GT888 was derived from GEM population DK888:N11 (GEMN-0177), which has 50% tro...

  1. Control system for the Spallation Neutron Source H{sup -} source test facility Allison scanner

    SciTech Connect

    Long, C. D.; Stockli, M. P.; Gorlov, T. V.; Han, B.; Murray, S. N.; Pennisi, T. R.

    2010-02-15

    Spallation Neutron Source is currently in progress of a multiyear plan to ramp ion beam power to the initial design power of 1.4 MW. Key to reaching this goal is understanding and improving the operation of the H{sup -} ion source. An Allison scanner was installed on the ion source in the test facility to support this improvement. This paper will discuss the hardware and the software control system of the installed Allison scanner. The hardware for the system consists of several parts. The heart of the system is the scanner head, complete with associated bias plates, slits, and signal detector. There are two analog controlled high voltage power supplies to bias the plates in the head, and a motor with associated controller to position the head in the beam. A multifunction data acquisition card reads the signals from the signal detector, as well as supplies the analog voltage control for the power supplies. To synchronize data acquisition with the source, the same timing signal that is used to trigger the source itself is used to trigger data acquisition. Finally, there is an industrial personal computer to control the rest of the hardware. Control software was developed using National Instruments LABVIEW, and consists of two parts: a data acquisition program to control the hardware and a stand alone application for offline user data analysis.

  2. Control System for the SNS H- Source Test Stand Allison Scanner

    SciTech Connect

    Long, Cary D; Stockli, Martin P; Gorlov, Timofey V; Han, Baoxi; Pennisi, Terry R; Murray Jr, S N

    2010-01-01

    SNS is currently in progress of a multi-year plan to ramp ion beam power to the initial design power of 1.4 MW. Key to reaching this goal is understanding and improving the operation of the H- ion source. An Allison scanner was installed on the ion source test stand to support this improvement. This paper will discuss the hardware and the software control system of the installed Allison scanner. The hardware for the system consists of several parts. The heart of the system is the scanner head, complete with associated bias plates, slits, and signal detector. There are two analog controlled high voltage power supplies to bias the plates in the head, and a motor with associated controller to position the head in the beam. A multifunction data acquisition card reads the signals from the signal detector, as well as supplying the analog voltage control for the power supplies. To synchronize data acquisition with the source, the same timing signal that is used to trigger the source itself is used to trigger data acquisition. Finally, there is an industrial PC to control the rest of the hardware. Control software was developed using National Instruments LabVIEW, and consists of two parts, a data acquisition program to control the hardware, and a stand alone application for offline user data analysis.

  3. THE ALLISON UNIT CO{sub 2}-ECBM PILOT: A RESERVOIR MODELING STUDY

    SciTech Connect

    Scott Reeves; Anne Taillefert; Larry Pekot; Chris Clarkson

    2003-02-01

    In October, 2000, the United States Department of Energy, through contractor Advanced Resources International (ARI), launched a multi-year government-industry research & development collaboration called the Coal-Seq project. The Coal-Seq project is investigating the feasibility of carbon dioxide (CO{sub 2}) sequestration in deep, unmineable coalseams by performing detailed reservoir studies of two enhanced coalbed methane (ECBM) recovery field projects in the San Juan basin. The two sites are the Allison Unit, operated by Burlington Resources, and into which CO{sub 2} is being injected, and the Tiffany Unit, operating by BP America, into which nitrogen (N{sub 2}) is being injected (the interest in understanding the N{sub 2}-ECBM process has important implications for CO{sub 2} sequestration via flue-gas injection). The purposes of the field studies are to understand the reservoir mechanisms of CO{sub 2} and N{sub 2} injection into coalseams, demonstrate the practical effectiveness of the ECBM and sequestration processes, demonstrate an engineering capability to model them, and to evaluate sequestration economics. In support of these efforts, laboratory and theoretical studies are also being performed to understand and model multicomponent isotherm behavior, and coal permeability changes due to swelling with CO{sub 2} injection. This report describes the results of an important component of the overall project, the Allison Unit reservoir study. The Allison Unit is located in the northern New Mexico portion of the prolific San Juan basin. The study area consists of 16 methane production wells, 4 CO{sub 2} injection wells, and one pressure observation well. The field originally began production in July 1989, and CO{sub 2} injection operations for ECBM purposes commenced in April, 1995. CO{sub 2} injection was suspended in August, 2001, to evaluate the results of the pilot. In this study, a detailed reservoir characterization of the field was developed, the field

  4. GT Strength in Odd-A Nuclei^*

    NASA Astrophysics Data System (ADS)

    Watson, J. W.; Du, Q. Q.

    1998-04-01

    We measured the complete set of polarization-transfer observables (D_ij) for the ^13C(p,n)^13N and ^15N(p,n)^15O reactions at 135 MeV. From the D_ijs we constructed the spin-independent, spin-longitudinal, and spin-transverse responses [1] D_0, D_q, Dn and D_p. From these responses we extracted, in a model-independent way, the Δ J=0 and Δ J=1 (``F'' and ``GT'') fractions of the J^π=1/2^-arrow1/2^- g.s. to g.s. transitions for these reactions. The ``F'' fraction, f_F=D_0(0^circ); the ``GT'' fraction, f_GT=D_q(0^circ)+D_n(0^circ)+D_p(0^circ)= 1- D_0(0^circ). The extracted GT fractions, f_GT, are substantially larger than one would predict from β-decay matrix elements and the systematics of the (p,n) reaction on even-A nuclei. These results confirm earlier, model-dependent determinations of f_GT obtained from the (p,n) reaction on ^13C, ^15N and ^39K at other energies [2], [3], [4], indicating that considerable caution must be used when extracting B(GT) matrix elements from odd-A (p,n) data. * Research supported in part by the U.S. NSF. [1] M. Ichimura, K. Kawahigashi, Phys. Rev. C 45 1822 (1992). [2] T. N. Taddeucci, C. A. Goulding, T. A. Carey, R. C. Byrd, C. D. Goodman, C. Gaarde, J. Larsen, D. Horen, J. Rapaport, and E. Sugarbaker, Nucl. Phys. A469 125 (1987). [3] H. Sakai, H. Okamura, N. Matsuoka, A. Shimizu, T. Suda, M. Ieiri and H. M. Shimizu, Nuclear Physics A579 45-61 (1994). [4] W. Huang, Ph.D. dissertation, Indiana U., 1991, (unpublished).

  5. GT Merge Process: Version 2.0

    SciTech Connect

    Flanagan, M P; Dodge, D; Myers, S C

    2008-06-10

    This document summarizes the process used to merge GT25 and better data between LANL and LLNL. The merge also includes OUO arrivals provided by AFTAC for events in the merge. The merge process is mostly automated and includes extensive quality control operations at each step. Events in common between the labs are identified and resolved using GT level criteria. Arrivals in common between the labs are also resolved through the use of agreed upon arrival author rankings. Finally, baselined origin times are computed for all crustal events using either teleseismic P-arrivals and the iasp91 model or, in certain regions, regional P-arrivals and regional velocity models that are known to be consistent with teleseismic iasp91 P-wave predictions. We combine the core tables from each contributor and resolve unique and common GT events between contributors. Next, we merge at the pick level so that each distinct EVENT-STATION-PHASE tuple has a unique arrival. All BMEB (Bondar-Myers-Engdahl-Bergman) GT are recalculated and evaluated for adherence to their criteria. Finally, new origin times are computed (baselining) for the merged GT events. In addition to the reconciliation of events and picks between contributors, the merge process involves several quality control steps that are intended to remove outlier and irrelevant data from the final results. The process is described in the section entitled 'Merge Steps'.

  6. GT Merge Process: Version 1.0

    SciTech Connect

    Flanagan, M P; Dodge, D; Myers, S C

    2008-06-10

    This document summarizes the process used to merge GT25 and better data between LANL and LLNL for use in a tomographic inversion for Pn velocity of Eurasia. The merge process is automated and includes extensive quality control operations at each step. Events in common between the labs are identified and resolved using GT level criteria. Arrivals in common between the labs are also resolved through the use of agreed upon arrival author rankings. Finally, baselined origin times are computed for all crustal events using either teleseismic P-arrivals and the iasp91 model or, in certain regions, regional P-arrivals and regional velocity models that are known to be consistent with teleseismic iasp91 P-wave predictions. We combine the core tables from each lab and first resolve unique and common GT events between LANL and LLNL. Phase names are then checked and possibly adjusted for consistency. Next, we merge at the pick level so that each distinct EVENT-STATION-PHASE tuple has a unique arrival. All BMEB (Bondar-Myers-Engdahl-Bergman) GT are evaluated for adherence to their criteria, and possibly re-calculated. Finally, new origin times are computed (baselining) for the merged GT events. In addition to the reconciliation of events and picks between LANL and LLNL, the merge process involves several quality control steps that are intended to remove outlier and irrelevant data from the final results.

  7. Comparative analysis of GT14/GT14-like family genes in Arabidopsis, Oryza, Populus, Sorghum and Vitis

    SciTech Connect

    Ye, Chuyu; Li, Ting; Tuskan, Gerald A; Tschaplinski, Timothy J; Yang, Xiaohan

    2011-01-01

    Glycosyltransferase family14 (GT14) belongs to the glycosyltransferase (GT) superfamily that plays important roles in the biosynthesis of cell walls, the most abundant source of cellulosic biomass for bioethanol production. It has been hypothesized that DUF266 proteins are a new class of GTs related to GT14. In this study, we identified 62 GT14 and 106 DUF266 genes (named GT14-like herein) in Arabidopsis, Oryza, Populus, Sorghum and Vitis. Our phylogenetic analysis separated GT14 and GT14-like genes into two distinct clades, which were further divided into eight and five groups, respectively. Similarities in protein domain, 3D structure and gene expression were uncovered between the two phylogenetic clades, supporting the hypothesis that GT14 and GT14-like genes belong to one family. Therefore, we proposed a new family name, GT14/GT14-like family that combines both subfamilies. Variation in gene expression and protein subcellular localization within the GT14-like subfamily were greater than those within the GT14 subfamily. One-half of the Arabidopsis and Populus GT14/GT14-like genes were found to be preferentially expressed in stem/xylem, indicating that they are likely involved in cell wall biosynthesis. This study provided new insights into the evolution and functional diversification of the GT14/GT14-like family genes.

  8. The Poplar GT8E and GT8F Glycosyltransferases are Functional Orthologs of Arabidopsis PARVUS Involved in Gulcuronoxylan Biosynthesis

    EPA Science Inventory

    The poplar GT8E and GT8F glycosyltransferases have previously been shown to be associated with wood formation, but their roles in the biosynthesis of wood components are not known. Here, we show that PoGT8E and PoGT8F are expressed in vessels and fibers during wood formation and ...

  9. Natural Hazard Problem and Solution Definition in the News Media: the Case of Tropical Storm Allison

    NASA Astrophysics Data System (ADS)

    Lindquist, Eric; Mosher-Howe, Katrina

    2010-05-01

    Focusing events such as natural or technological disasters can have significant impacts on public policy and planning in both the near and long term. These impacts can manifest at different temporal scales ranging from the period of immediate attention and disaster relief through the period of recovery and reconstruction and beyond. These impacts and associated decisions can be studied in retrospect and understood as not only short-term reactions, but as long-term components of subsequent natural hazard planning and public policy. By studying in detail how an event was defined, and the policy and planning alternatives that were raised or recommended in response to a disaster event, we can better understand the role that disaster-related focusing events play in the long-term evolution of a community's public policy, infrastructural planning efforts, and responses to natural disasters. This paper will use a focusing event framework to explore the local and regional policy impacts over time of a major urban flood in Houston, Texas, Tropical Storm Allison. Tropical Storm Allison (TSA), dropped 36 inches of rain on Houston over a period of four days in early June 2001, and was responsible for 22 deaths, 70,000 flood damaged homes, and 5 billion in damage to the region. The primary data source for this effort is a database of 500 articles from the major regional newspaper, the Houston Chronicle, over the period of 2001 through 2008. These articles were coded for multiple variables, including, cause, effect and impact (financial and social), blame, problem and solution definition and solution acceptance). This paper focuses primarily on the measures of problem definition (how was TSA, as an event, defined in the media, for example, as an act of God, or as a result of poor planning or decision making, etc), and on solution definition (what solutions were proposed to mitigate or adapt to future storms of this magnitude, how were they linked to the definition of the problem

  10. James P. Allison received the 2014 Szent-Györgi Prize for Progress in Cancer Research

    PubMed Central

    Zhao, Jie; Scully, Peter; Ba, Sujuan

    2014-01-01

    The Szent-Györgyi Prize for Progress in Cancer Research is a prestigious scientific award established by the National Foundation for Cancer Research (NFCR)—a leading cancer research charitable organization in the United States that is committed to supporting innovative cancer research on the global scale that aims to cure cancer. Each year, the Szent-Györgyi Prize honors an outstanding researcher whose original discoveries have expanded our understanding of cancer and resulted in notable advances in cancer prevention, diagnosis, or treatment. The prize also promotes public awareness of the importance of basic cancer research and encourages the sustained investment needed to accelerate the translation of these research discoveries into new cancer treatments. This report highlights the history and mission of the Szent-Györgyi Prize, its role in promoting discovery-oriented cancer research, and the pioneering work led by the 2014 prize winner, Dr. James Allison. Dr. Allison's work in the area of cancer immunotherapy led to the successful development of immune checkpoint therapy, and the first drug approved by the United States Food and Drug Administration for the treatment of metastatic melanoma. PMID:25189714

  11. The Wheat GT Factor TaGT2L1D Negatively Regulates Drought Tolerance and Plant Development

    PubMed Central

    Zheng, Xin; Liu, Haipei; Ji, Hongtao; Wang, Youning; Dong, Baodi; Qiao, Yunzhou; Liu, Mengyu; Li, Xia

    2016-01-01

    GT factors are trihelix transcription factors that specifically regulate plant development and stress responses. Recently, several GT factors have been characterized in different plant species; however, little is known about the role of GT factors in wheat. Here, we show that TaGT2L1A, TaGT2L1B, and TaGT2L1D are highly homologous in hexaploid wheat, and are localized to wheat chromosomes 2A, 2B, and 2D, respectively. These TaGT2L1 genes encode proteins containing two SANT domains and one central helix. All three homologs were ubiquitously expressed during wheat development and were responsive to osmotic stress. Functional analyses demonstrated that TaGT2L1D acts as a transcriptional repressor; it was able to suppress the expression of AtSDD1 in Arabidopsis by binding directly to the GT3 box in its promoter that negatively regulates drought tolerance. TaGT2L1D overexpression markedly increased the number of stomata and reduced drought tolerance in gtl1-3 plants. Notably, ectopic expression of TaGT2L1D also affected floral organ development and overall plant growth. These results demonstrate that TaGT2L1 is an ortholog of AtGTL1, and that it plays an evolutionarily conserved role in drought resistance by fine tuning stomatal density in wheat. Our data also highlight the role of TaGT2L1 in plant growth and development. PMID:27245096

  12. Erosion Coatings for High-Temperature Polymer Composites: A Collaborative Project With Allison Advanced Development Company

    NASA Technical Reports Server (NTRS)

    Sutter, James K.

    2000-01-01

    composite (ASTM D 4541 95 "Pull Off Strength of Coatings"). Glenn and Allison Advanced Development Company collaborated to optimize erosion coatings for gas turbine fan and compressor applications. All the coating systems survived aggressive thermal cycling without spalling. During erosion tests (see the final photo), the most promising coating systems tested had Cr3C2-NiCr and WC-Co as the hard topcoats. In all cases, these coating systems performed significantly better than that with a TiN hard topcoat. When material depth (thickness) loss is considered, the Cr3C2-NiCr and WC-Co coating systems provided, on average, an erosion resistance 8.5 times greater than that for the uncoated PMR 15/T650 35 composite. Similarly, Cr3C2-NiCr and WC-Co coating systems adhered to the PMC substrate during tensile tests significantly better than systems containing a TiN topcoat. Differences in topcoats of Cr3C2-NiCr and WC-Co were determined by considering issues such as cost and environmental impact. The preferred erosion-resistant coating system for PMR 15/T650 35 has WC-Co as the hard topcoat. This system provides the following benefits in comparison to the coating system with Cr3C2-NiCr topcoat: lower powder material cost (15 to 20 percent), environmentally friendly materials (Cr3C2-NiCr is hazardous), and higher deposition yield (10 to 15 percent), which results in less waste.

  13. High antagonist potency of GT-2227 and GT-2331, new histamine H3 receptor antagonists, in two functional models.

    PubMed

    Tedford, C E; Hoffmann, M; Seyedi, N; Maruyama, R; Levi, R; Yates, S L; Ali, S M; Phillips, J G

    1998-06-26

    GT-2227 (4-(6-cyclohexylhex-cis-3-enyl)imidazole) and GT-2331 ((1R,2R)-4-(2-(5,5-dimethylhex-1-ynyl)cyclopropyl)imidazole) were developed as new potent histamine H3 receptor antagonists. The functional activity of these ligands on the histamine H3 receptor-mediated inhibition of neurogenic contraction of the guinea-pig jejunum and histamine H3 receptor-mediated inhibition of norepinephrine release from guinea-pig heart synaptosomes were investigated. GT-2227 and GT-2331 both antagonized the inhibitory effects of (R)-alpha-methylhistamine on the contraction induced by electrical field stimulation in the guinea-pig jejunum with pA2 values of 7.9+/-0.1 and 8.5+/-0.03, respectively. In addition, GT-2227 and GT-2331 antagonized the inhibition of norepinephrine release in cardiac synaptosomes by GT-2203 ((1R,2R)-trans-2-(1H-imidazol-4-yl)cyclopropylamine), a histamine H3 receptor agonist. The current results demonstrate the antagonist activity for both GT-2227 and GT-2331 in two functional assays for histamine H3 receptors.

  14. Technical Reliability Assessment of the Actigraph GT1M Accelerometer

    ERIC Educational Resources Information Center

    Silva, Pedro; Mota, Jorge; Esliger, Dale; Welk, Gregory

    2010-01-01

    The purpose of this study was to determine the reliability of the Actigraph GT1M (Pensacola, FL, USA) accelerometer activity count and step functions. Fifty GT1M accelerometers were initialized to collect simultaneous acceleration counts and steps data using 15-sec epochs. All reliability testing was completed using a mechanical shaker plate to…

  15. 3. AIR TO GROUND RADAR TYPE GT2122 & GRRR 2324, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. AIR TO GROUND RADAR TYPE GT2122 & GRRR 2324, CIRCA 1978, INTERIOR OF BUILDING 408, LOOKING WEST. - Mill Valley Air Force Station, Operations Building & Annex, East Ridgecrest Boulevard, Mount Tamalpais, Mill Valley, Marin County, CA

  16. HTGR-GT and electrical load integrated control

    SciTech Connect

    Chan, T.; Openshaw, F.; Pfremmer, D.

    1980-05-01

    A discussion of the control and operation of the HTGR-GT power plant is presented in terms of its closely coupled electrical load and core cooling functions. The system and its controls are briefly described and comparisons are made with more conventional plants. The results of analyses of selected transients are presented to illustrate the operation and control of the HTGR-GT. The events presented were specifically chosen to show the controllability of the plant and to highlight some of the unique characteristics inherent in this multiloop closed-cycle plant.

  17. [NRC/GT: Six Year One Research Studies.

    ERIC Educational Resources Information Center

    Gubbins, E. Jean, Ed.

    1992-01-01

    This newsletter focuses on six Year 1 research projects associated with the National Research Center on the Gifted and Talented (NRC/GT). The updates address: "Regular Classroom Practices With Gifted Students: Findings from the Classroom Practices Survey" (Francis X. Archambault, Jr. and others); "The Classroom Practices Study:…

  18. The Development of the CryoTel™ LT and GT

    NASA Astrophysics Data System (ADS)

    Unger, Reuven

    2006-04-01

    This paper describes the considerations, design modifications and test results for the recently-developed Sunpower CryoTel™ LT and GT. After successful market introduction of the CryoTel™ CT, it became evident that some basic design modifications would render the unit available to a wider range of application. The CryoTel™ LT is a low-temperature variant of the original design. The LT's cooling capacity is 0.5W @ 23 K. The CryoTel™ GT is the enhanced performance variant. The GT's cooling capacity is 15 W @ 77 K. Both LT and GT largely retain the original structure and components and therefore benefit from the low-cost manufacturing profile of the original. Sunpower's main analysis and simulation tools were in-house codes and Gedeon Associates' SAGE Stirling cycle simulation. The CryoTel™ is a Linear Free Piston Integral Stirling cryocooler that makes use of Gas Bearing technology for non-contact operation and a microprocessor based driver/controller with a closed-loop temperature control.

  19. GT2RDF: Semantic Representation of Genetic Testing Data

    PubMed Central

    Paul Rupa, Anamika; Singh, Sweta; Zhu, Qian

    2016-01-01

    Accelerated by the Human Genome Project, genetic testing has become an increasingly integral component in diagnosis, treatment, management, and prevention of numerous diseases and conditions. More than 480 laboratories perform genetic tests for more than 4,600 rare and common medical conditions. These tests can effectively help health professionals to determine or predict the genetic conditions of their patients. However, physicians have not actively incorporated such innovative genetic technology into their clinical practices according to two national wide surveys commissioned by UnitedHealth Group. To fill the gap of insufficient use of a large number of genetic tests, we generated a single Resource Description Framework (RDF) resource, called GT2RDF (Genetic Testing data to RDF) by integrating information about disease, gene, phenotype, genetic test, and drug from multiple sources including Genetic Testing Registry (GTR), Online Mendelian Inheritance in Man (OMIM), MedGen, Human Phenotype Ontology (HPO), ClinVar, National Drug File Reference Terminology (NDF-RT). Meanwhile, we manually annotated and extracted information from 200 randomly selected GeneReviews chapters, and integrated into the GT2RDF. We performed two case studies to demonstrate the usability of the GT2RDF. GT2RDF will serve as a data foundation to support the design of a genetic testing recommendation system, called iGenetics, which will ultimately facilitate the pace of precision medicine by means of actively and effectively incorporating innovative genetic technology in clinical settings. Abbreviations: GT2RDF: Genetic Testing data to RDF; SWT: Semantic web technology; OWL: Ontology Web Language; RDF: Resource Description Framework; SPARQL: SPARQL Protocol and RDF Query Language; GTR: Genetic Testing Registry; OMIM: Online Mendelian Inheritance in Man; HPO: Human Phenotype Ontology; NDF-RT: National Drug File Reference Terminology; UMLS: Unified Medical Language System. PMID:28269903

  20. GT2RDF: Semantic Representation of Genetic Testing Data.

    PubMed

    Paul Rupa, Anamika; Singh, Sweta; Zhu, Qian

    2016-01-01

    Accelerated by the Human Genome Project, genetic testing has become an increasingly integral component in diagnosis, treatment, management, and prevention of numerous diseases and conditions. More than 480 laboratories perform genetic tests for more than 4,600 rare and common medical conditions. These tests can effectively help health professionals to determine or predict the genetic conditions of their patients. However, physicians have not actively incorporated such innovative genetic technology into their clinical practices according to two national wide surveys commissioned by UnitedHealth Group. To fill the gap of insufficient use of a large number of genetic tests, we generated a single Resource Description Framework (RDF) resource, called GT2RDF (Genetic Testing data to RDF) by integrating information about disease, gene, phenotype, genetic test, and drug from multiple sources including Genetic Testing Registry (GTR), Online Mendelian Inheritance in Man (OMIM), MedGen, Human Phenotype Ontology (HPO), ClinVar, National Drug File Reference Terminology (NDF-RT). Meanwhile, we manually annotated and extracted information from 200 randomly selected GeneReviews chapters, and integrated into the GT2RDF. We performed two case studies to demonstrate the usability of the GT2RDF. GT2RDF will serve as a data foundation to support the design of a genetic testing recommendation system, called iGenetics, which will ultimately facilitate the pace of precision medicine by means of actively and effectively incorporating innovative genetic technology in clinical settings. Abbreviations: GT2RDF: Genetic Testing data to RDF; SWT: Semantic web technology; OWL: Ontology Web Language; RDF: Resource Description Framework; SPARQL: SPARQL Protocol and RDF Query Language; GTR: Genetic Testing Registry; OMIM: Online Mendelian Inheritance in Man; HPO: Human Phenotype Ontology; NDF-RT: National Drug File Reference Terminology; UMLS: Unified Medical Language System.

  1. GT-CATS: Tracking Operator Activities in Complex Systems

    NASA Technical Reports Server (NTRS)

    Callantine, Todd J.; Mitchell, Christine M.; Palmer, Everett A.

    1999-01-01

    Human operators of complex dynamic systems can experience difficulties supervising advanced control automation. One remedy is to develop intelligent aiding systems that can provide operators with context-sensitive advice and reminders. The research reported herein proposes, implements, and evaluates a methodology for activity tracking, a form of intent inferencing that can supply the knowledge required for an intelligent aid by constructing and maintaining a representation of operator activities in real time. The methodology was implemented in the Georgia Tech Crew Activity Tracking System (GT-CATS), which predicts and interprets the actions performed by Boeing 757/767 pilots navigating using autopilot flight modes. This report first describes research on intent inferencing and complex modes of automation. It then provides a detailed description of the GT-CATS methodology, knowledge structures, and processing scheme. The results of an experimental evaluation using airline pilots are given. The results show that GT-CATS was effective in predicting and interpreting pilot actions in real time.

  2. Turbulent transport measurements in a model of GT-combustor

    NASA Astrophysics Data System (ADS)

    Chikishev, L. M.; Gobyzov, O. A.; Sharaborin, D. K.; Lobasov, A. S.; Dulin, V. M.; Markovich, D. M.; Tsatiashvili, V. V.

    2016-10-01

    To reduce NOx formation modern industrial power gas-turbines utilizes lean premixed combustion of natural gas. The uniform distribution of local fuel/air ratio in the combustion chamber plays one of the key roles in the field of lean combustion to prevent thermo-acoustic pulsations. Present paper reports on simultaneous Particle Image Velocimetry and acetone Planar Laser Induced Fluorescence measurements in a cold model of GT-combustor to investigate mixing processes which are relevant to the organization of lean premixed combustion. Velocity and passive admixture pulsations correlations were measured to verify gradient closer model, which is often used in Reynolds-Averaged Navier-Stokes (RANS) simulation of turbulent mixing.

  3. Helium turbomachine design for GT-MHR power plant

    SciTech Connect

    McDonald, C.F.; Orlando, R.J.; Cotzas, G.M.

    1994-07-01

    The power conversion system in the gas turbine modular helium reactor (GT-MHR) power plant is based on a highly recuperated closed Brayton cycle. The major component in the direct cycle system is a helium closed-cycle gas turbine rated at 286 MW(e). The rotating group consists of an intercooled helium turbocompressor coupled to a synchronous generator. The vertical rotating assembly is installed in a steel vessel, together with the other major components (i.e., recuperator, precooler, intercooler, and connecting ducts and support structures). The rotor is supported on an active magnetic bearing system. The turbine operates directly on the reactor helium coolant, and with a temperature of 850{degree}C (1562{degree}F) the plant efficiency is over 47%. This paper addresses the design and development planning of the helium turbomachine, and emphasizes that with the utilization of proven technology, this second generation nuclear power plant could be in service in the first decade of the 21st century.

  4. A photometric and orbital analysis of GT MUSCAE

    NASA Astrophysics Data System (ADS)

    Murdoch, K. A.; Hearnshaw, J. B.; Kilmartin, P. M.; Gilmore, A. C.

    1995-10-01

    GT Mus is a quadruple system comprising a long-period RS CVn-type binary (HD 101379) and a pair of eclipsing A dwarfs (HD 101380). Six and a half years of UBV (RI)_C photometry obtained at the Mt John University Observatory has enabled identification of four distinct types of photometric variability in this system. These are (1) a slowly changing mean magnitude, which probably arises from an activity-cycle-like effect in the active component of HD 101379, (2) a periodic variation (P_rot~64d), which is attributed to rotational modulation due to spots on the active star, (3) a periodic variation (P_eclipse=2.7546d) due to the eclipses of HD 101380, and (4) an excess in the I band, which occurs on a short time-scale (<1d) and is probably associated with HD 101379 activity. The evolution of the light curve of HD 101379 is fast with respect to the rotational period, suggesting rapid spot evolution for which we anticipate a possible model. The colours of HD 101379, even at maximum brightness, are excessively red for its spectral type, unless there is significant reddening by dust. Radial velocity measurements of HD 101379 are also presented, along with an improved determination of the orbit of this somewhat long-period (P_orb=61.448d) system.

  5. The National Research Center on the Gifted and Talented (NRC/GT) Newsletter, 1998.

    ERIC Educational Resources Information Center

    Gubbins, E. Jean, Ed.; Siegle, Del, Ed.

    1998-01-01

    These two newsletters of The National Research Center on the Gifted and Talented (NRC/GT) present articles concerned with research on the education of gifted and talented students. The articles are: "NRC/GT's Suggestions: Evaluating Your Programs and Services" (E. Jean Gubbins); "Professional Development Practices in Gifted Education: Results of a…

  6. The National Research Center on the Gifted and Talented (NRC/GT) Newsletter, 1999.

    ERIC Educational Resources Information Center

    Gubbins, E. Jean, Ed.; Siegle, Del, Ed.

    1999-01-01

    These two newsletters of The National Research Center on the Gifted and Talented (NRC/GT) present articles concerned with research on the education of gifted and talented students. The articles include: "NRC/GT: Making Decisions and Determining Next Steps" (E. Jean Gubbins); "Free Summer Programs for Talented Teens" (D. Betsy McCoach); "High End…

  7. Late Paleocene-middle Eocene benthic foraminifera on a Pacific seamount (Allison Guyot, ODP Site 865): Greenhouse climate and superimposed hyperthermal events

    NASA Astrophysics Data System (ADS)

    Arreguín-Rodríguez, Gabriela J.; Alegret, Laia; Thomas, Ellen

    2016-03-01

    We investigated the response of late Paleocene-middle Eocene (~60-37.5 Ma) benthic foraminiferal assemblages to long-term climate change and hyperthermal events including the Paleocene-Eocene Thermal Maximum (PETM) at Ocean Drilling Program (ODP) Site 865 on Allison Guyot, a seamount in the Mid-Pacific Mountains. Seamounts are isolated deep-sea environments where enhanced current systems interrupt bentho-pelagic coupling, and fossil assemblages from such settings have been little evaluated. Assemblages at Site 865 are diverse and dominated by cylindrical calcareous taxa with complex apertures, an extinct group which probably lived infaunally. Dominance of an infaunal morphogroup is unexpected in a highly oligotrophic setting, but these forms may have been shallow infaunal suspension feeders, which were ecologically successful on the current-swept seamount. The magnitude of the PETM extinction at Site 865 was similar to other sites globally, but lower diversity postextinction faunas at this location were affected by ocean acidification as well as changes in current regime, which might have led to increased nutrient supply through trophic focusing. A minor hyperthermal saw less severe effects of changes in current regime, with no evidence for carbonate dissolution. Although the relative abundance of infaunal benthic foraminifera has been used as a proxy for surface productivity through bentho-pelagic coupling, we argue that this proxy can be used only in the absence of changes in carbonate saturation and current-driven biophysical linking.

  8. GT-2: in vivo transcriptional activation activity and definition of novel twin DNA binding domains with reciprocal target sequence selectivity.

    PubMed

    Ni, M; Dehesh, K; Tepperman, J M; Quail, P H

    1996-06-01

    GT-2 is a novel DNA binding protein that interacts with a triplet functionally defined, positively acting GT-box motifs (GT1-bx, GT2-bx, and GT3-bx) in the rice phytochrome A gene (PHYA) promoter. Data from a transient transfection assay used here show that recombinant GT-2 enhanced transcription from both homologous and heterologous GT-box-containing promoters, thereby indicating that this protein can function as a transcriptional activator in vivo. Previously, we have shown that GT-2 contains separate DNA binding determinants in its N- and C-terminal halves, with binding site preferences for the GT3-bx and GT2-bx promoter motifs, respectively. Here, we demonstrate that the minimal DNA binding domains reside within dual 90-amino acid polypeptide segments encompassing duplicated sequences, termed trihelix regions, in each half of the molecule, plus 15 additional immediately adjacent amino acids downstream. These minimal binding domains retained considerable target sequence selectivity for the different GT-box motifs, but this selectivity was enhanced by a separate polypeptide segment farther downstream on the C-terminal side of each trihelix region. Therefore, the data indicate that the twin DNA binding domains of GT-2 each consist of a general GT-box recognition core with intrinsic differential binding activity toward closely related target motifs and a modified sequence conferring higher resolution reciprocal selectivity between these motifs.

  9. GT160-246, a toxin binding polymer for treatment of Clostridium difficile colitis.

    PubMed

    Kurtz, C B; Cannon, E P; Brezzani, A; Pitruzzello, M; Dinardo, C; Rinard, E; Acheson, D W; Fitzpatrick, R; Kelly, P; Shackett, K; Papoulis, A T; Goddard, P J; Barker, R H; Palace, G P; Klinger, J D

    2001-08-01

    GT160-246, a high-molecular-weight soluble anionic polymer, was tested in vitro and in vivo for neutralization of Clostridium difficile toxin A and B activities. Five milligrams of GT160-246 per ml neutralized toxin-mediated inhibition of protein synthesis in Vero cells induced by 5 ng of toxin A per ml or 1.25 ng of toxin B per ml. In ligated rat ileal loops, 1 mg of GT160-246 neutralized fluid accumulation caused by 5 microg of toxin A. At doses as high as 80 mg/loop, cholestyramine provided incomplete neutralization of fluid accumulation caused by 5 microg of toxin A. GT160-246 protected 80% of the hamsters from mortality caused by infection with C. difficile, whereas cholestyramine protected only 10% of animals. Treatment of C. difficile-infected hamsters with metronidazole initially protected 100% of the hamsters from mortality, but upon removal of treatment, 80% of the hamsters had relapses and died. In contrast, removal of GT160-246 treatment did not result in disease relapse in the hamsters. GT160-246 showed no antimicrobial activity in tests with a panel of 16 aerobic bacteria and yeast and 22 anaerobic bacteria and did not interfere with the in vitro activities of most antibiotics. GT160-246 offers a novel, nonantimicrobial treatment of C. difficile disease in humans.

  10. Development of Orally Administered γ-Tocotrienol (GT3) Nanoemulsion for Radioprotection

    PubMed Central

    Ledet, Grace A.; Biswas, Shukla; Kumar, Vidya P.; Graves, Richard A.; Mitchner, Demaurian M.; Parker, Taylor M.; Bostanian, Levon A.; Ghosh, Sanchita P.; Mandal, Tarun K.

    2016-01-01

    The purpose of this study was two-fold: (1) to formulate γ-tocotrienol (GT3) in a nanoemulsion formulation as a prophylactic orally administered radioprotective agent; and (2) to optimize the storage conditions to preserve the structural integrity of both the formulation and the compound. γ-tocotrienol was incorporated into a nanoemulsion and lyophilized with lactose. Ultra performance liquid chromatography–mass spectroscopy (UPLC–MS) was used to monitor the chemical stability of GT3 over time, the particle size and ζ potential, and scanning electron microscopy (SEM) were used to study the physical stability of the nanoemulsion. Radioprotective and toxicity studies were performed in mice. The liquid formulation exhibited GT3 degradation at all storage temperatures. Lyophilization, in the presence of lactose, significantly reduced GT3 degradation. Both the liquid and lyophilized nanoemulsions had stable particle size and ζ potential when stored at 4 °C. Toxicity studies of the nanoemulsion resulted in no observable toxicity in mice at an oral dose of 600 mg/kg GT3. The nano-formulated GT3 (300 mg/kg) demonstrated enhanced survival efficacy compared to GT3 alone (200 and 400 mg/kg) in CD2F1 mice exposed to total body gamma radiation. The optimal long-term storage of formulated GT3 is as a powder at −20 °C to preserve drug and formulation integrity. Formulation of GT3 as a nanoemulsion for oral delivery as a prophylactic radioprotectant shows promise and warrants further investigation. PMID:28029115

  11. Multi-color lightcurve observation of the asteroid (163249) 2002 GT

    NASA Astrophysics Data System (ADS)

    Oshima, M.; Abe, S.

    2014-07-01

    NASA's Deep Impact/EPOXI spacecraft plans to encounter the asteroid (163249) 2002 GT, classified as a PHA (Potentially Hazardous Asteroid), on January 4, 2020. However, the taxonomic type and spin state of 2002 GT remain to be determined. We have carried out ground-based multi-color (B-V-R-I) lightcurve observations taking advantage of the 2002 GT Characterization Campaign by NASA. Multi-color lightcurve measurements allow us to estimate the rotation period and obtain strong constraints on the shape and pole orientation. Here we found that the rotation period of 2002 GT is estimated to be 3.7248 ± 0.1664 h. In mid-2013, 2002 GT passed at 0.015 au from the Earth, resulting an exceptional opportunity for ground-based characterization. Using the 0.81-m telescope of the Tenagra Observatory (110°52'44.8''W, +31°27'44.4''N, 1312 m) in Arizona, USA, and the Johnson-Cousins BVRI filters, we have found lightcurves of 2002 GT (Figure). The Tenagra II 0.81-m telescope is used for research of the Hayabusa2 target Asteroid (162173) 1999 JU_3. The lightcurves (relative magnitude) show that the rotation period of 2002 GT, the target of NASA's Deep Impact/EPOXI spacecraft, is estimated to be 3.7248 ± 0.1664 hr. On June 9, 2013, we had 7 hours of ground-based observations on 2002 GT from 4:00 to 11:00 UTC. The number of comparison stars for differential photometry was 34. Because of tracking the fast-moving asteroid, it was necessary to have the same comparison star among the fields of vision. We have also obtained absolute photometry of 2002 GT on June 13, 2013.

  12. Dynamics and control modeling of the closed-cycle gas turbine (GT-HTGR) power plant

    SciTech Connect

    Bardia, A.

    1980-02-01

    The simulation if presented for the 800-MW(e) two-loop GT-HTGR plant design with the REALY2 transient analysis computer code, and the modeling of control strategies called for by the inherently unique operational requirements of a multiple loop GT-HTGR is described. Plant control of the GT-HTGR is constrained by the nature of its power conversion loops (PCLs) in which the core cooling flow and the turbine flow are directly related and thus changes in flow affect core cooling as well as turbine power. Additionally, the high thermal inertia of the reactor core precludes rapid changes in the temperature of the turbine inlet flow.

  13. Energy recuperation in solid oxide fuel cell (SOFC) and gas turbine (GT) combined system

    NASA Astrophysics Data System (ADS)

    Kuchonthara, Prapan; Bhattacharya, Sankar; Tsutsumi, Atsushi

    A combined power generation system consisting of a solid oxide fuel cell (SOFC) and a gas turbine (GT) with steam and heat recuperation (HR) was evaluated using a commercial process simulation tool, ASPEN Plus. The effect of steam recuperation (SR) on the overall efficiency of the combined system was investigated by comparing the SOFC-GT during heat and steam recuperation (HSR) against the system during only heat recuperation. At low turbine inlet temperatures (TITs), the overall efficiency of the SOFC-GT combined system with heat and steam recuperation improved by showing an increase in TIT and a reduction in pressure ratio (PR). On the other hand, at high TITs, the opposite trend was observed. The integration of steam recuperation was found to improve the overall efficiency and specific power of SOFC-GT combined systems with a relatively compact SOFC component.

  14. [Cloning and functional analysis of the cotton Trihelix transcription factor GhGT29].

    PubMed

    Yue, Li; Xiaodong, Liu; Yongmei, Dong; Zongming, Xie; Shouyi, Chen

    2015-12-01

    Trihelix transcription factors are important proteins involved in response to abiotic stresses in plants. Understanding the molecular mechanisms of Trihelix in cottons will lay the foundation to improve stress tolerance by gene engineering. In this study, a gene encoding Trihelix transcription factor was isolated in upland cottons using reverse transcription PCR according to bioinformatic analysis. The gene was named as GhGT29 (GenBank accession No. JQ013097), which was 1 092 bp, contained a 1 089 bp open reading frame and encoded a protein of 363 amino acids with a predicted molecular weight of 40.9 kDa and a isoelectric point of 5.45. SMART analysis showed GhGT29 contained one typical SANT motif. Phylogenetic analysis showed that GhGT29 belonged to the SH4 subfamily of the Trihelix family and was most closely related to AtSH4-like1 and AtSH4-like2. Quantitative real-time PCR (qRT-PCR) analysis revealed that GhGT29 was induced by high salt, drought, cold and abscisic acid. The expression profile also revealed that GhGT29 was constitutively expressed in all tested tissues, such as roots, stems, leaves, flowers, ovules (0 DPA) and fibers (12 DPA). The expression level of GhGT29 was the highest in flowers and the lowest in stems. Using the Arabidopsis protoplasts assay system, we found that the GhGT29 protein was located in cell nuclei and had trans-activation activity. These results revealed that GhGT29 might be involved in the regulation of stress resistance-related genes in stress signaling pathways in upland cottons.

  15. Genetically modified flax expressing NAP-SsGT1 transgene: examination of anti-inflammatory action.

    PubMed

    Matusiewicz, Magdalena; Kosieradzka, Iwona; Zuk, Magdalena; Szopa, Jan

    2014-09-22

    The aim of the work was to define the influence of dietary supplementation with GM (genetically modified) GT#4 flaxseed cake enriched in polyphenols on inflammation development in mice liver. Mice were given ad libitum isoprotein diets: (1) standard diet; (2) high-fat diet rich in lard, high-fat diet enriched with 30% of (3) isogenic flax Linola seed cake; and (4) GM GT#4 flaxseed cake; for 96 days. Administration of transgenic and isogenic seed cake lowered body weight gain, of transgenic to the standard diet level. Serum total antioxidant status was statistically significantly improved in GT#4 flaxseed cake group and did not differ from Linola. Serum thiobarbituric acid reactive substances, lipid profile and the liver concentration of pro-inflammatory cytokine tumor necrosis factor-α were ameliorated by GM and isogenic flaxseed cake consumption. The level of pro-inflammatory cytokine interferon-γ did not differ between mice obtaining GM GT#4 and non-GM flaxseed cakes. The C-reactive protein concentration was reduced in animals fed GT#4 flaxseed cake and did not differ from those fed non-GM flaxseed cake-based diet. Similarly, the liver structure of mice consuming diets enriched in flaxseed cake was improved. Dietetic enrichment with GM GT#4 and non-GM flaxseed cakes may be a promising solution for health problems resulting from improper diet.

  16. Sleep quality in efavirenz-treated Chinese HIV patients - comparing between GT and GG genotype of CYP2B6-516 G/T polymorphisms.

    PubMed

    Lee, Shui Shan; To, Kin Wang; Lee, Man Po; Wong, Ngai Sze; Chan, Denise P C; Li, Patrick C K; Cheung, Siu Wai; Chan, Raphael C Y

    2014-03-01

    Seventy-two adult Chinese HIV-positive treatment-naïve patients were recruited in a study to evaluate prospectively the associations between CYP2B6 516 G/T polymorphisms and sleep quality following treatment with an efavirenz-based regimen. Overall, the patients gave an allelic frequency of 0.3 for CYP2B6 516 T, and a genotype frequency of 9.4% for TT. Compared to GG, GT gave a higher median value of plasma efavirenz level at four weeks (3.77 mg/L vs 2.59 mg/L, p < 0.001) and 12 months (3.57 mg/L vs 2.97 mg/L, p = 0.026). Using generalised estimating equations analysis to track the variance over time, there was poorer Pittsburgh Sleep Quality Index in GT compared to GG, while GT was associated with a higher efavirenz level of >4 mg/L. There was however no difference in the component sleep scores nor was there direct association between sleep quality and plasma efavirenz levels. The results suggested that CYP2B6 genotype was associated with different patterns of sleep problems, further investigation of which is warranted with the objective of optimizing therapy with efavirenz-based regimens.

  17. Family 34 glycosyltransferase (GT34) genes and proteins in Pinus radiata (radiata pine) and Pinus taeda (loblolly pine).

    PubMed

    Ade, Carsten P; Bemm, Felix; Dickson, James M J; Walter, Christian; Harris, Philip J

    2014-04-01

    Using a functional genomics approach, four candidate genes (PtGT34A, PtGT34B, PtGT34C and PtGT34D) were identified in Pinus taeda. These genes encode CAZy family GT34 glycosyltransferases that are involved in the synthesis of cell-wall xyloglucans and heteromannans. The full-length coding sequences of three orthologs (PrGT34A, B and C) were isolated from a xylem-specific cDNA library from the closely related Pinus radiata. PrGT34B is the ortholog of XXT1 and XXT2, the two main xyloglucan (1→6)-α-xylosyltransferases in Arabidopsis thaliana. PrGT34C is the ortholog of XXT5 in A. thaliana, which is also involved in the xylosylation of xyloglucans. PrGT34A is an ortholog of a galactosyltransferase from fenugreek (Trigonella foenum-graecum) that is involved in galactomannan synthesis. Truncated coding sequences of the genes were cloned into plasmid vectors and expressed in a Sf9 insect cell-culture system. The heterologous proteins were purified, and in vitro assays showed that, when incubated with UDP-xylose and cellotetraose, cellopentaose or cellohexaose, PrGT34B showed xylosyltransferase activity, and, when incubated with UDP-galactose and the same cello-oligosaccharides, PrGT34B showed some galactosyltransferase activity. The ratio of xylosyltransferase to galactosyltransferase activity was 434:1. Hydrolysis of the galactosyltransferase reaction products using galactosidases showed the linkages formed were α-linkages. Analysis of the products of PrGT34B by MALDI-TOF MS showed that up to three xylosyl residues were transferred from UDP-xylose to cellohexaose. The heterologous proteins PrGT34A and PrGT34C showed no detectable enzymatic activity.

  18. Ganglioside GT1b protects human spermatozoa from hydrogen peroxide-induced DNA and membrane damage.

    PubMed

    Gavella, Mirjana; Garaj-Vrhovac, Verica; Lipovac, Vaskresenija; Antica, Mariastefania; Gajski, Goran; Car, Nikica

    2010-06-01

    We have reported previously that various gangliosides, the sialic acid containing glycosphingolipids, provide protection against sperm injury caused by reactive oxygen species (ROS). In this study, we investigated the effect of treatment of human spermatozoa with ganglioside GT1b on hydrogen peroxide (H(2)O(2))-induced DNA fragmentation and plasma membrane damage. Single-cell gel electrophoresis (Comet assay) used in the assessment of sperm DNA integrity showed that in vitro supplemented GT1b (100 microm) significantly reduced DNA damage induced by H(2)O(2) (200 microm) (p < 0.05). Measurements of Annexin V binding in combination with the propidium iodide vital dye labelling demonstrated that the spermatozoa pre-treated with GT1b exhibited a significant increase (p < 0.05) in the percentage of live cells with intact membrane and decreased phosphatidylserine translocation after exposure to H(2)O(2). Flow cytometry using the intracellular ROS-sensitive fluorescence dichlorodihydrofluorescein diacetate dye employed to investigate the transport of the extracellularly supplied H(2)O(2) into the cell interior revealed that ganglioside GT1b completely inhibited the passage of H(2)O(2) through the sperm membrane. These results suggest that ganglioside GT1b may protect human spermatozoa from H(2)O(2)-induced damage by rendering sperm membrane more hydrophobic, thus inhibiting the diffusion of H(2)O(2) across the membrane.

  19. Bacterial β-Kdo glycosyltransferases represent a new glycosyltransferase family (GT99)

    PubMed Central

    Ovchinnikova, Olga G.; Mallette, Evan; Koizumi, Akihiko; Lowary, Todd L.; Kimber, Matthew S.

    2016-01-01

    Kdo (3-deoxy-d-manno-oct-2-ulosonic acid) is an eight-carbon sugar mostly confined to Gram-negative bacteria. It is often involved in attaching surface polysaccharides to their lipid anchors. α-Kdo provides a bridge between lipid A and the core oligosaccharide in all bacterial LPSs, whereas an oligosaccharide of β-Kdo residues links “group 2” capsular polysaccharides to (lyso)phosphatidylglycerol. β-Kdo is also found in a small number of other bacterial polysaccharides. The structure and function of the prototypical cytidine monophosphate-Kdo–dependent α-Kdo glycosyltransferase from LPS assembly is well characterized. In contrast, the β-Kdo counterparts were not identified as glycosyltransferase enzymes by bioinformatics tools and were not represented among the 98 currently recognized glycosyltransferase families in the Carbohydrate-Active Enzymes database. We report the crystallographic structure and function of a prototype β-Kdo GT from WbbB, a modular protein participating in LPS O-antigen synthesis in Raoultella terrigena. The β-Kdo GT has dual Rossmann-fold motifs typical of GT-B enzymes, but extensive deletions, insertions, and rearrangements result in a unique architecture that makes it a prototype for a new GT family (GT99). The cytidine monophosphate-binding site in the C-terminal α/β domain closely resembles the corresponding site in bacterial sialyltransferases, suggesting an evolutionary connection that is not immediately evident from the overall fold or sequence similarities. PMID:27199480

  20. Performance of Allison Model 400-C6 Turbojet-Engine Compressor. I; Over-All Performance Characteristics of Compressor at Equivalent Impeller Speeds of 6000, 7000, and 8500 RPM

    NASA Technical Reports Server (NTRS)

    Kovach, Karl; Withee, Joseph R., Jr.

    1948-01-01

    The Allison model 400-C6 compressor was operated at an inlet pressure of 12 inches of mercury absolute ana ambient inlet temperature at equivalent impeller speeds of 6000, 7000, and 8500 rpm. Additional runs at an equivalent speed of 7000 rpm and ambient inlet temperature were made at inlet pressures from 7 to 22 inches of mercury absolute. The results of this investigation are compared with those of the 533-A-23 compressors. For the speeds investigated, the Allison model 400-C6 compressor had a maximum adiabatic temperature-rise efficiency of 0.768 at an equivalent speed of 7000 rpm; the corresponding equivalent weight flow was 45.0 pounds per second and the pressure ratio was 1.83. At an equivalent impeller speed of 8500 rpm, the maximum equivalent weight flow was 61.6 pounds per second and the peak pressure ratio of 2.38 occurred at an equivalent weight flow of 52.2 pounds per 1 second and an adiabatic temperature-rise efficiency of 0.714. At an equivalent speed of 7000 rpm, increasing the compressor- inlet pressure increased the maximum equivalent weight flow and the pressure ratio.

  1. LU60645GT and MA132843GT Catalogues of Lunar and Martian Impact Craters Developed Using a Crater Shape-based Interpolation Crater Detection Algorithm for Topography Data

    NASA Technical Reports Server (NTRS)

    Salamuniccar, Goran; Loncaric, Sven; Mazarico, Erwan Matias

    2012-01-01

    For Mars, 57,633 craters from the manually assembled catalogues and 72,668 additional craters identified using several crater detection algorithms (CDAs) have been merged into the MA130301GT catalogue. By contrast, for the Moon the most complete previous catalogue contains only 14,923 craters. Two recent missions provided higher-quality digital elevation maps (DEMs): SELENE (in 1/16° resolution) and Lunar Reconnaissance Orbiter (we used up to 1/512°). This was the main motivation for work on the new Crater Shape-based interpolation module, which improves previous CDA as follows: (1) it decreases the number of false-detections for the required number of true detections; (2) it improves detection capabilities for very small craters; and (3) it provides more accurate automated measurements of craters' properties. The results are: (1) LU60645GT, which is currently the most complete (up to D>=8 km) catalogue of Lunar craters; and (2) MA132843GT catalogue of Martian craters complete up to D>=2 km, which is the extension of the previous MA130301GT catalogue. As previously achieved for Mars, LU60645GT provides all properties that were provided by the previous Lunar catalogues, plus: (1) correlation between morphological descriptors from used catalogues; (2) correlation between manually assigned attributes and automated measurements; (3) average errors and their standard deviations for manually and automatically assigned attributes such as position coordinates, diameter, depth/diameter ratio, etc; and (4) a review of positional accuracy of used datasets. Additionally, surface dating could potentially be improved with the exhaustiveness of this new catalogue. The accompanying results are: (1) the possibility of comparing a large number of Lunar and Martian craters, of e.g. depth/diameter ratio and 2D profiles; (2) utilisation of a method for re-projection of datasets and catalogues, which is very useful for craters that are very close to poles; and (3) the extension of the

  2. Optimizing the G/T ratio of the DSS-13 34-meter beam-waveguide antenna

    NASA Technical Reports Server (NTRS)

    Esquivel, M. S.

    1992-01-01

    Calculations using Physical Optics computer software were done to optimize the gain-to-noise temperature (G/T) ratio of DSS-13, the DSN's 34-m beam-waveguide antenna, at X-band for operation with the ultra-low-noise amplifier maser system. A better G/T value was obtained by using a 24.2-dB far-field-gain smooth-wall dual-mode horn than by using the standard X-band 22.5-dB-gain corrugated horn.

  3. Optimizing the G/T ratio of the DSS-13 34-meter beam-waveguide antenna

    NASA Technical Reports Server (NTRS)

    Esquivel, M. S.

    1992-01-01

    Calculations using Physical Optics computer software were done to optimize the gain-to-noise-temperature (G/T) ratio of Deep Space Station (DSS)-13, the Deep Space Network's (DSN's) 34-m beam-waveguide antenna, at X-band for operation with the ultra-low-noise amplifier maser system. A better G/T value was obtained by using a 24.2-dB far-field-gain smooth-wall dual-mode horn than by using the standard X-band 22.5-dB-gain corrugated horn.

  4. The "Invisible" Gifted and Talented Bilingual Students: A Current Report on Enrollment in GT Programs

    ERIC Educational Resources Information Center

    Esquierdo, J. Joy; Arreguin-Anderson, Maria

    2012-01-01

    The issue of underrepresentation in gifted and talented (GT) programs has developed into a critical educational concern. At the core are ambiguous identification assessment practices, especially for bilingual students. To illustrate, this article reports data from the last decade that supports the underrepresentation of gifted Hispanic bilingual…

  5. Comparison of Yamax pedometer and GT3X accelerometer steps in a free-living sample

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Our objective was to compare steps detected by the Yamax pedometer (PEDO) versus the GT3X accelerometer (ACCEL) in free-living adults. Daily PEDO and ACCEL steps were collected from a sample of 23 overweight and obese participants (18 females; mean +/- sd: age = 52.6 +/- 8.4 yr.; body mass index = 3...

  6. 78 FR 29810 - Receipt of Petition for Decision That Nonconforming 2003 BMW K 1200 GT Motorcycles Are Eligible...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-21

    ... 2003 BMW K 1200 GT Motorcycles Are Eligible for Importation AGENCY: National Highway Traffic Safety... Traffic Safety Administration (NHTSA) of a petition for a decision that 2003 BMW K 1200 GT Motorcycles... (Registered Importer R-09-005) has petitioned NHTSA to decide whether non-U.S. certified 2003 BMW K 1200...

  7. 48 CFR 915.404-4 - Profit.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) and Bonneville Power Administration (BPA) functions. Pursuant to section 602(d) (13) and (20) of the... as those of the BPA, now being performed by DOE are exempt from the 6 percent of cost restriction...

  8. 48 CFR 915.404-4 - Profit.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) and Bonneville Power Administration (BPA) functions. Pursuant to section 602(d) (13) and (20) of the... as those of the BPA, now being performed by DOE are exempt from the 6 percent of cost restriction...

  9. 48 CFR 915.404-4 - Profit.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) and Bonneville Power Administration (BPA) functions. Pursuant to section 602(d) (13) and (20) of the... as those of the BPA, now being performed by DOE are exempt from the 6 percent of cost restriction...

  10. 48 CFR 915.404-4 - Profit.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) and Bonneville Power Administration (BPA) functions. Pursuant to section 602(d) (13) and (20) of the... as those of the BPA, now being performed by DOE are exempt from the 6 percent of cost restriction...

  11. 48 CFR 15.404-4 - Profit.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... Considerations include the diversity of engineering, scientific, and manufacturing labor skills required and the... owned and controlled by socially and economically disadvantaged individuals, women-owned small...

  12. 48 CFR 15.404-4 - Profit.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    .... Considerations include the diversity of engineering, scientific, and manufacturing labor skills required and the... owned and controlled by socially and economically disadvantaged individuals, women-owned small...

  13. 48 CFR 315.404-4 - Profit.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... profit objective is that part of the estimated contract price objective or value which, in the judgment... departure from this objective. The amount of documentation depends on the dollar value and complexity of the... of the value of each factor, the Contracting Officer shall consider the definition, description,...

  14. 48 CFR 315.404-4 - Profit.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... profit objective is that part of the estimated contract price objective or value which, in the judgment... departure from this objective. The amount of documentation depends on the dollar value and complexity of the... of the value of each factor, the Contracting Officer shall consider the definition, description,...

  15. 48 CFR 215.404-4 - Profit.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Federally Funded Research and Development Centers (FFRDCs) (see 215.404-75). There are three structured... relevant variables have not materially changed (e.g., performance risk, interest rates, progress...

  16. 48 CFR 215.404-4 - Profit.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... perform a profit analysis when assessing cost realism in competitive acquisitions. (2) When using a...., performance risk, interest rates, progress payment rates, distribution of facilities capital). (d)...

  17. 48 CFR 315.404-4 - Profit.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... circumstances: (A) Contracts not expected to exceed $100,000. (B) A & E contracts. (C) Management contracts for... contract performance, takes into account what resources are necessary and what steps the contractor must... appropriate evaluation technique. (C) The Contracting Officer shall consider management problems that...

  18. 48 CFR 15.404-4 - Profit.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... differences in the categories used by prospective contractors for listing costs— (A) Material acquisition... parts and material, subcontracted items, and special tooling. Considerations include the complexity of..., whether established sources are available or new or second sources must be developed, and whether...

  19. 48 CFR 15.404-4 - Profit.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... differences in the categories used by prospective contractors for listing costs— (A) Material acquisition... parts and material, subcontracted items, and special tooling. Considerations include the complexity of..., whether established sources are available or new or second sources must be developed, and whether...

  20. 48 CFR 315.404-4 - Profit.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... circumstances: (A) Contracts not expected to exceed $100,000. (B) A & E contracts. (C) Management contracts for... requiring delivery of material supplies by subcontractors (F) Termination settlements. (G) Cost-plus-award...: Material acquisition 1 to 5. Direct labor 4 to 15. Overhead 4 to 9. General & Administrative (G & A) 4 to...

  1. 48 CFR 915.404-4 - Profit.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... followed, except as exempted for DOE architect-engineer contracts covering Atomic Energy Commission (AEC... contracts for architect-engineer services. The estimated costs on which the maximum fee is computed shall... profit/fee analysis technique designed for a systematic application of the profit factors in FAR...

  2. 48 CFR 15.404-4 - Profit.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of the estimated cost of construction of the public work or utility, excluding fees. (C) For other...) Conversion direct labor. This subfactor measures the contribution of direct engineering, manufacturing, and.... Considerations include the diversity of engineering, scientific, and manufacturing labor skills required and...

  3. Gonadotropic hormone (GtH) receptors in the testis of the troutSalmo gairdneri: in vitro studies.

    PubMed

    Le Gac, F; Breton, B; Bougoussa, M

    1988-10-01

    A particulate fraction obtained from trout testis at the time of spermiation shows saturable binding sites for(125)I-labeled salmon gonadotropin ((125)I-GtH). Non-gonadal tissues (liver, muscle and spleen) did not demonstrate specific(125)I-GtH binding. The tracer's specific activity was determined by the self-displacement method (18 to 30 μCi/μg). Maximal specific binding ability of(125)I-GtH varied from 20 to 30% of the labelled ligand added, depending on the hormone preparation. Specific binding of(125)I-GtH to 20 mg of the testis membrane varied from 40 to 85% of the total binding depending on the method of membrane prepratation, and was competitively inhibited by concentrations of unlabelled GtH ranging from ca 1 to 1000 ng/ml of incubate. Gonadotropin of mammalian origin, ovine TSH or salmon prolactin competed only weakly, or not at all, for testicular gonadotropin binding sites (relative potencies s-GtH>FSH=hCG>s-PRL>bTSH). Scatchard analysis of equilibrium binding studies shows that saturable gonadotropin binding was due to a class of high affinity binding sites (sites I Ka≊3×10(10) M(-1)) and possibly to a second class of lower affinity binding sites (sites II Ka=5 to 14×10(8) M(-1)). The binding capacity of sites I, as measured in enriched membrane preparations, was 45±18 fmoles/g of testis during the period of spermiation. The concentration of GtH required to obtain half maximal displacement of(125)I-GtH in the binding studies was of the same order of magnitude as the apparent ED50 for GtH stimulation of 11-Cetotestosterone (11KT) secretion by trout testesin vitro. Mammalian LH and FSH were 100 to 1000 folds less potent than salmor GtH to increase 11 KT secretion.

  4. The DBA Analysis of One Control Rod Withdrawal Out of the HTR-10GT Core

    SciTech Connect

    Mingang Lang; Yujie Dong

    2006-07-01

    The 10 MW High Temperature Gas Cooled Test Reactor (HTR-10) has been built in Institute of Nuclear and New Energy Technology (INET) and has been operating successfully since the beginning of 2003. The core outlet temperature of HTR-10 is 700 deg. C. To verify the technology of gas-turbine direct cycle, INET has planned to increase its core outlet temperature to 750 deg. C and use a helium gas turbine instead of the steam generator (then the reactor is called HTR-10GT). Though HTR-10 has good intrinsic safety, the design basic accidents and beyond design basic accidents of HTR10-GT must be analyzed according to China's nuclear regulations due to changed operation parameters. THERMIX code system is used to study the accident on one control rod withdrawal out of the core by a mistake. After a control rod in the side reflector was withdrawn out at a speed of 1 cm/s by a mistake, a positive reactivity was inserted and the reactor power increased and the temperature of the core increased. When the neutron flux of power measuring range exceeded 123% and the core outlet temperature was lager than 800 deg. C, the reactor was scrammed. During the accident sequence the maximum fuel temperature was 1200.9 deg. C. It was lower than the fuel temperature limitation of 1230 deg. C. The paper compares the analysis result of HTR10-GT to those of HTR-10. The results shows that the HTR-10GT is still safe during the accident though its operating temperature is higher than HTR-10 when the fuel safety limits are the same. (authors)

  5. Observing Campaign for Potential Deep Impact Flyby Target 163249 (2002 GT)

    NASA Technical Reports Server (NTRS)

    Pittichova, Jana; Chesley, S. R.; Abell, P. A.; Benner, L. A. M.

    2012-01-01

    The Deep Impact spacecraft is currently on course for a Jan. 4, 2020 flyby of the sub-kilometer near-Earth asteroid 163249 (2002 GT). The re-targeting will be complete with a final small maneuver scheduled for Oct. 4, 2012. 2002 GT, which is also designated as a Potentially Hazardous Asteroid (PHA), has a well-determined orbit and is approx 800 m in diameter (H=18.3). Little more is known about the nature of this object, but in mid-2013 it will pass near the Earth, affording an exceptional opportunity for ground-based characterization. At this apparition 2002 GT will be in range of Arecibo. In addition to Doppler measurements, radar delay observations with precisions of a few microseconds are expected and have a good chance of revealing whether the system is binary or not. The asteroid will be brighter than 16th mag., which will facilitate a host of observations at a variety of wavelengths. Light curve measurements across a wide range of viewing perspectives will reveal the rotation rate and ultimately lead to strong constraints on the shape and pole orientation. Visible and infrared spectra will constrain the mineralogy, taxonomy, albedo and size. Along with the radar observations, optical astrometry will further constrain the orbit, both to facilitate terminal guidance operations and to potentially reveal nongravitational forces acting on the asteroid. Coordinating all of these observations will be a significant task and we encourage interested observers to collaborate in this effort. The 2013 apparition of 2002 GT represents a unique opportunity to characterize a potential flyby target, which will aid interpretation of the high-resolution flyby imagery and aid planning and development of the flyby imaging sequence. The knowledge gained from this flyby will be highly relevant to the human exploration program at NASA, which desires more information on the physical characteristics of sub-kilometer near-Earth asteroids.

  6. Thermal Emission Photometry of Deep Impact Flyby Target (163249) 2002 GT

    NASA Astrophysics Data System (ADS)

    Lim, Lucy F.; Moskovitz, N. A.; Licandro, J.; Emery, J. P.; Reddy, V.; Vilas, F.; 2002 GT Observing Team

    2013-10-01

    Near-Earth asteroid (163249) 2002 GT is now the target of a Deep Impact spacecraft flyby in Jan. 2020 (see Pittichova et al., this volume, for details of the flyby and observing campaign). Thermal emission photometry of 2002 GT was obtained from NIRI on Gemini-North in the L' and M' filters, which are centered at 3.76 and 4.68 microns respectively. J- and K-band reflectance photometry was also acquired in support of the thermal observations. The full JKL'M' set was acquired on UT 2013-Jun-13 at a solar phase angle of 53 degrees. A further set of photometry in J, K, and L' only was carried out on 2013-Jun-19 at a phase angle of 65 degrees. High water vapor conditions at Mauna Kea during this period unfortunately prevented acquisition of a second set of M' measurements. In addition, N-band photometry of 2002 GT was conducted on 2013-Jun-10 from CanariCam at the 10-meter Gran Telescopio Canarias using a beta version of the moving object guiding system. Data were acquired in three filters between 8.7 and 12.5 microns, although the limitations of the guiding are complicating the analysis. (We note that N-band observing was not offered by either Gemini or IRTF during this apparition.) Data analysis is ongoing and results will be discussed. We appreciate the efforts of the Gemini and GTC staff in support of these observing programs.

  7. The Vitamin E Analog Gamma-Tocotrienol (GT3) and Statins Synergistically Up-Regulate Endothelial Thrombomodulin (TM)

    PubMed Central

    Pathak, Rupak; Ghosh, Sanchita P.; Zhou, Daohong; Hauer-Jensen, Martin

    2016-01-01

    Statins; a class of routinely prescribed cholesterol-lowering drugs; inhibit 3-hydroxy-3-methylglutaryl-coenzymeA reductase (HMGCR) and strongly induce endothelial thrombomodulin (TM); which is known to have anti-inflammatory; anti-coagulation; anti-oxidant; and radioprotective properties. However; high-dose toxicity limits the clinical use of statins. The vitamin E family member gamma-tocotrienol (GT3) also suppresses HMGCR activity and induces TM expression without causing significant adverse side effects; even at high concentrations. To investigate the synergistic effect of statins and GT3 on TM; a low dose of atorvastatin and GT3 was used to treat human primary endothelial cells. Protein-level TM expression was measured by flow cytometry. TM functional activity was determined by activated protein C (APC) generation assay. Expression of Kruppel-like factor 2 (KLF2), one of the key transcription factors of TM, was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). TM expression increased in a dose-dependent manner after both atorvastatin and GT3 treatment. A combined treatment of a low-dose of atorvastatin and GT3 synergistically up-regulated TM expression and functional activity. Finally; atorvastatin and GT3 synergistically increased KLF2 expression. These findings suggest that combined treatment of statins with GT3 may provide significant health benefits in treating a number of pathophysiological conditions; including inflammatory and cardiovascular diseases. PMID:27869747

  8. MTP -493G>T polymorphism and susceptibility to nonalcoholic fatty liver disease: a meta-analysis.

    PubMed

    Zheng, Wei; Wang, Lu; Su, Xiao; Hu, Xiao-Fang

    2014-06-01

    Microsomal transfer protein (MTP), a lipid transfer protein localized in the endoplasmic reticulum of hepatocytes and enterocytes, plays an important role in the development of nonalcoholic fatty liver disease (NAFLD). Many existing studies have demonstrated that a common polymorphism (-493G>T, rs1800591 G>T) in the MTP gene may be implicated in the development and progression of NAFLD, but individually published results are inconclusive. This meta-analysis aimed to investigate whether MTP -493G>T polymorphism may be a potential risk factor for NAFLD. We searched CISCOM, CINAHL, Web of Science, PubMed, Google Scholar, EBSCO, Cochrane Library, and CBM databases from inception through October 1, 2013. Meta-analysis was performed using the STATA 12.0 software. Eleven clinical case-control studies with a total of 636 NAFLD cases and 918 healthy controls met the inclusion criteria. Our meta-analysis results revealed that MTP -493G>T polymorphism was strongly correlated with an increased risk of NAFLD. Subgroup analysis by ethnicity suggested that MTP -493G>T polymorphism might increase individuals' susceptibility to NAFLD among both Caucasian and non-Caucasian populations. No publication bias was observed in this meta-analysis. In short, the present meta-analysis indicates that MTP -493G>T polymorphisms may contribute to individuals' susceptibility to NAFLD. Thus, MTP -493G>T polymorphism may be a valuable and practical biomarker for early detection of NAFLD.

  9. Feasibility study for SOFC-GT hybrid locomotive power part II. System packaging and operating route simulation

    NASA Astrophysics Data System (ADS)

    Martinez, Andrew S.; Brouwer, Jacob; Samuelsen, G. Scott

    2012-09-01

    This work assesses the feasibility of Solid Oxide Fuel Cell-Gas Turbine (SOFC-GT) hybrid power systems for use as the prime mover in freight locomotives. The available space in a diesel engine-powered locomotive is compared to that required for an SOFC-GT system, inclusive of fuel processing systems necessary for the SOFC-GT. The SOFC-GT space requirement is found to be similar to current diesel engines, without consideration of the electrical balance of plant. Preliminary design of the system layout within the locomotive is carried out for illustration. Recent advances in SOFC technology and implications of future improvements are discussed as well. A previously-developed FORTRAN model of an SOFC-GT system is then augmented to simulate the kinematics and power notching of a train and its locomotives. The operation of the SOFC-GT-powered train is investigated along a representative route in Southern California, with simulations presented for diesel reformate as well as natural gas reformate and hydrogen as fuels. Operational parameters and difficulties are explored as are comparisons of expected system performance to modern diesel engines. It is found that even in the diesel case, the SOFC-GT system provides significant savings in fuel and CO2 emissions, making it an attractive option for the rail industry.

  10. Biosynthesis of the major brain gangliosides GD1a and GT1b

    PubMed Central

    Sturgill, Elizabeth R; Aoki, Kazuhiro; Lopez, Pablo HH; Colacurcio, Daniel; Vajn, Katarina; Lorenzini, Ileana; Majić, Senka; Yang, Won Ho; Heffer, Marija; Tiemeyer, Michael; Marth, Jamey D; Schnaar, Ronald L

    2012-01-01

    Gangliosides—sialylated glycosphingolipids—are the major glycoconjugates of nerve cells. The same four structures—GM1, GD1a, GD1b and GT1b—comprise the great majority of gangliosides in mammalian brains. They share a common tetrasaccharide core (Galβ1–3GalNAcβ1-4Galβ1-4Glcβ1-1′Cer) with one or two sialic acids on the internal galactose and zero (GM1 and GD1b) or one (GD1a and GT1b) α2–3-linked sialic acid on the terminal galactose. Whereas the genes responsible for the sialylation of the internal galactose are known, those responsible for terminal sialylation have not been established in vivo. We report that St3gal2 and St3gal3 are responsible for nearly all the terminal sialylation of brain gangliosides in the mouse. When brain ganglioside expression was analyzed in adult St3gal1-, St3gal2-, St3gal3- and St3gal4-null mice, only St3gal2-null mice differed significantly from wild type, expressing half the normal amount of GD1a and GT1b. St3gal1/2-double-null mice were no different than St3gal2-single-null mice; however, St3gal2/3-double-null mice were >95% depleted in gangliosides GD1a and GT1b. Total ganglioside expression (lipid-bound sialic acid) in the brains of St3gal2/3-double-null mice was equivalent to that in wild-type mice, whereas total protein sialylation was reduced by half. St3gal2/3-double-null mice were small, weak and short lived. They were half the weight of wild-type mice at weaning and displayed early hindlimb dysreflexia. We conclude that the St3gal2 and St3gal3 gene products (ST3Gal-II and ST3Gal-III sialyltransferases) are largely responsible for ganglioside terminal α2-3 sialylation in the brain, synthesizing the major brain gangliosides GD1a and GT1b. PMID:22735313

  11. Biosynthesis of the major brain gangliosides GD1a and GT1b.

    PubMed

    Sturgill, Elizabeth R; Aoki, Kazuhiro; Lopez, Pablo H H; Colacurcio, Daniel; Vajn, Katarina; Lorenzini, Ileana; Majić, Senka; Yang, Won Ho; Heffer, Marija; Tiemeyer, Michael; Marth, Jamey D; Schnaar, Ronald L

    2012-10-01

    Gangliosides-sialylated glycosphingolipids-are the major glycoconjugates of nerve cells. The same four structures-GM1, GD1a, GD1b and GT1b-comprise the great majority of gangliosides in mammalian brains. They share a common tetrasaccharide core (Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-1'Cer) with one or two sialic acids on the internal galactose and zero (GM1 and GD1b) or one (GD1a and GT1b) α2-3-linked sialic acid on the terminal galactose. Whereas the genes responsible for the sialylation of the internal galactose are known, those responsible for terminal sialylation have not been established in vivo. We report that St3gal2 and St3gal3 are responsible for nearly all the terminal sialylation of brain gangliosides in the mouse. When brain ganglioside expression was analyzed in adult St3gal1-, St3gal2-, St3gal3- and St3gal4-null mice, only St3gal2-null mice differed significantly from wild type, expressing half the normal amount of GD1a and GT1b. St3gal1/2-double-null mice were no different than St3gal2-single-null mice; however, St3gal2/3-double-null mice were >95% depleted in gangliosides GD1a and GT1b. Total ganglioside expression (lipid-bound sialic acid) in the brains of St3gal2/3-double-null mice was equivalent to that in wild-type mice, whereas total protein sialylation was reduced by half. St3gal2/3-double-null mice were small, weak and short lived. They were half the weight of wild-type mice at weaning and displayed early hindlimb dysreflexia. We conclude that the St3gal2 and St3gal3 gene products (ST3Gal-II and ST3Gal-III sialyltransferases) are largely responsible for ganglioside terminal α2-3 sialylation in the brain, synthesizing the major brain gangliosides GD1a and GT1b.

  12. HTGR-GT closed-cycle gas turbine: a plant concept with inherent cogeneration (power plus heat production) capability

    SciTech Connect

    McDonald, C.F.

    1980-04-01

    The high-grade sensible heat rejection characteristic of the high-temperature gas-cooled reactor-gas turbine (HTGR-GT) plant is ideally suited to cogeneration. Cogeneration in this nuclear closed-cycle plant could include (1) bottoming Rankine cycle, (2) hot water or process steam production, (3) desalination, and (4) urban and industrial district heating. This paper discusses the HTGR-GT plant thermodynamic cycles, design features, and potential applications for the cogeneration operation modes. This paper concludes that the HTGR-GT plant, which can potentially approach a 50% overall efficiency in a combined cycle mode, can significantly aid national energy goals, particularly resource conservation.

  13. Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development

    PubMed Central

    HWANG, Seon-Ung; JEON, Yubyeol; YOON, Junchul David; CAI, Lian; KIM, Eunhye; YOO, Hyunju; KIM, Kyu-Jun; PARK, Kyu Mi; JIN, Minghui; KIM, Hyunggee; HYUN, Sang-Hwan

    2015-01-01

    Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P < 0.05) different between the control group and the 5 nM GT1b treatment group. Intracellular glutathione (GSH) levels in oocytes matured with 5 nM and 20 nM and GT1b decreased significantly (P < 0.05). The 10 nM group showed a significant (P < 0.05) decrease in intracellular reactive oxygen species (ROS) levels compared with the control group. Subsequently, the level of intracellular Ca2+ in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca2+ was significantly (P < 0.05) increased with a higher concentration of GT1b in a dose-dependent manner. Real-time PCR was performed and showed that the expression of bradykinin 2 receptor (B2R) and calcium/calmodulin-dependent protein kinase II delta (CaMKIIδ) in cumulus cells was significantly (P < 0.05) decreased in the 20 nM GT1b treatment group. Treatment with 5 nM GT1b significantly (P < 0.05) decreased the expression of CaMKIIδ. In oocytes, treatment with 5 nM GT1b significantly (P < 0.05) decreased CaMKIIγ and POU5F1 (POU domain, class 5, transcription factor 1). However, treatment with 20 nM GT1b significantly (P < 0.05) increased the expression of POU5F1. Finally, embryonic developmental data showed no significant differences in the two experiments (parthenogenesis and in vitro fertilization). In conclusion, the results of the present study indicated that GT1b plays an important role in increasing the nuclear maturation rate and decreasing the intracellular ROS levels during IVM. However, GT1b inhibited maturation of the cytoplasm by maintaining intracellular Ca2+ in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism

  14. Apical extrusion of thermoplasticized obturating material in canals instrumented with Profile 0.06 or Profile GT.

    PubMed

    Robinson, Mark J; McDonald, N J; Mullally, Patrick J

    2004-06-01

    The purpose of this in vitro study was to compare the extrusion of thermoplacticized gutta-percha in teeth instrumented with Profile 0.06 or Profile GT, and obturated with Thermafil Plus and Thermafil GT, respectively. A total of 120, extracted, human maxillary central incisors were divided into four equal groups. Group 1 was instrumented with Profile 0.06 and obturated with Thermafil Plus. Group 2 was instrumented with Profile 0.06 and obturated using warm vertical condensation (negative control). Group 3 was instrumented with Profile GT and obturated with Thermafil GT. Group 4 was instrumented with Profile GT and obturated like Group 2 (negative control). Extrusion was graded as present or absent. Results found 9 of 30 extruded for group 1, 1 of 30 for group 2, 15 of 30 for group 3, and 2 of 30 for group 4. The results suggest that, in vitro, Thermafil GT may be more prone to extruding gutta-percha past the apical foramen than Thermafil Plus.

  15. Designing Ground Antennas for Maximum G/T: Cassegrain or Gregorian?

    NASA Technical Reports Server (NTRS)

    Imbriale, William A.

    2005-01-01

    For optimum performance, a ground antenna system must maximize the ratio of received signal to the receiving system noise power, defined as the ratio of antenna gain to system-noise temperature (G/T). The total system noise temperature is the linear combination of the receiver noise temperature (including the feed system losses) and the antenna noise contribution. Hence, for very low noise cryogenic receiver systems, antenna noise-temperature properties are very significant contributors to G/T.It is well known that, for dual reflector systems designed for maximum gain, the gain performance of the antenna system is the same for both Cassegrain and Gregorian configurations. For a12-meter antenna designed to be part of the large array based Deep Space Network, a Cassegrain configuration designed for maximum G/T at X-band was 0.7 dB higher than the equivalent Gregorian configuration. This study demonstrates that, for maximum GIT, the dual shaped Cassegrain design is always better than the Gregorian.

  16. Subunit profiling and functional characteristics of acetylcholine receptors in GT1-7 cells.

    PubMed

    Arai, Yuki; Ishii, Hirotaka; Kobayashi, Makito; Ozawa, Hitoshi

    2017-03-01

    GnRH neurons form a final common pathway for the central regulation of reproduction. Although the involvement of acetylcholine in GnRH secretion has been reported, direct effects of acetylcholine and expression profiles of acetylcholine receptors (AChRs) still remain to be studied. Using immortalized GnRH neurons (GT1-7 cells), we analyzed molecular expression and functionality of AChRs. Expression of the mRNAs were identified in the order α7 > β2 = β1 ≧ α4 ≧ α5 = β4 = δ > α3 for nicotinic acetylcholine receptor (nAChR) subunits and m4 > m2 for muscarinic acetylcholine receptor (mAChR) subtypes. Furthermore, this study revealed that α7 nAChRs contributed to Ca(2+) influx and GnRH release and that m2 and m4 mAChRs inhibited forskolin-induced cAMP production and isobutylmethylxanthine-induced GnRH secretion. These findings demonstrate the molecular profiles of AChRs, which directly contribute to GnRH secretion in GT1-7 cells, and provide one possible regulatory action of acetylcholine in GnRH neurons.

  17. Sayarim Infrasound Calibration Explosion provides first GT0 dataset for CTBTO

    NASA Astrophysics Data System (ADS)

    Gitterman, Yefim

    2010-05-01

    The large-scale calibration explosion of about 82 tons of HE explosives, assembled as a pyramid on the soft sediment surface, was successfully conducted by the Geophysical Institute of Israel at Sayarim Military Range on 26 August 2009. High-pressure values, measured in the range 200-600 m, were higher than predicted, whereas the created crater and seismic magnitude were smaller than expected for this on-surface charge. These results confirm that the used explosives, charge design and upward detonation provided the necessary explosion energy generation and partition: maximum of energy to the atmosphere and minimum to the ground. The high-pressure observations were utilized for estimation of the important Ground Truth parameter - TNT equivalent yield of about 0.1 kT (based on positive impulse in air-shock wave). Thus the Sayarim Explosion provided the first full GT0 source dataset for on-surface large-scale explosions, recorded by infrasound stations of International Monitoring System (IMS). Infrasound signals were well observed at distances up to 3,500 km, at numerous portable and permanent stations in Israel, Mediterranean countries and north-central Europe, including two IMS stations I26DE and I48TN and two portable arrays in Austria and Northern Italy deployed by the CTBTO team. Obtained records were used for analysis of infrasound signal propagation, source location and yield estimation, and comparison with GT0 source parameters.

  18. Microsatellite (GT)(n) repeats and SNPs in the von Willebrand factor gene promoter do not influence circulating von Willebrand factor levels under normal conditions.

    PubMed

    Daidone, Viviana; Cattini, Maria Grazia; Pontara, Elena; Sartorello, Francesca; Gallinaro, Lisa; Marotti, Alberto; Scaroni, Carla; Pagnan, Antonio; Casonato, Alessandra

    2009-02-01

    Von Willebrand factor (VWF) levels vary considerably in normal individuals, influenced by inherited and acquired modulators. ABO blood group is the major inherited determinant of VWF levels, but a role has also been attributed to the VWF gene promoter, haplotype 1 (-3268G/-2709C/-2661A/-2527G) being associated with higher VWF levels than haplotype 2 (-3268C/-2709T/-2661G/-2527A), and the polymorphic locus (GT)(n) modulating the shear stress-induced activation of the VWF promoter. We characterized the (GT)(n) of the VWF promoter in 394 healthy individuals and assessed whether its variable length influenced VWF levels in normal conditions. (GT)(n) proved highly polymorphic, with alleles from 15 to 24 repeats long. (GT)(21) and (GT)(19) were the most common variants (37.4% and 34.4%, respectively). Short GT repeats (15-19) segregated mainly with haplotype 1, long GT repeats (20-24) with haplotype 2 (p < 0.0001). The number of GT repeats did not correlate with VWF levels, nor did such levels correlate with haplotypes 1 and 2, considered alone or in association with the (GT)(n) locus. We conclude that (GT)(n) and -3268/-2709/-2661/-2527 loci are in strong linkage disequilibrium. This polymorphic region of the VWF promoter does not affect VWF levels under normal conditions, though it might represent an environmentally activable VWF regulation site.

  19. Correlation between MTP -493G>T polymorphism and non-alcoholic fatty liver disease risk: a meta-analysis.

    PubMed

    Li, L; Wang, S J; Shi, K; Chen, D; Jia, H; Zhu, J

    2014-12-04

    Several studies have found that microsomal transfer protein (MTP) may be important in the development and progression of non-alcoholic fatty liver disease (NAFLD). In this meta-analysis, we evaluated the relationships between a common polymorphism (-493G>T, rs1800591 G>T) in the MTP gene and NAFLD risk. The PubMed, CISCOM, CINAHL, Web of Science, Google Scholar, EBSCO, Cochrane Library, and CBM databases were searched for relevant articles published before October 1, 2013 without any language restrictions. Meta-analysis was conducted using the STATA 12.0 software. Crude odds ratios (ORs) with 95% confidence intervals (95%CIs) were calculated. Eleven case-control studies were included in this meta-analysis. A total of 636 NAFLD patients and 918 healthy control subjects were examined in this meta-analysis. Our results indicate that the MTP -493G/T polymorphism increases the risk of NAFLD (G allele vs T allele: OR = 1.39, 95%CI = 1.17-1.65, P < 0.001; GG + GT vs TT: OR = 1.46, 95%CI = 1.02-2.09, P = 0.038, respectively). Subgroup analyses indicated that the MTP -493G/T polymorphism was associated with an increased risk of NAFLD in population-based, hospital-based, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and large sample-size subgroups under the allele and dominant models (all P < 0.05). However, we found no association between non-PCR-RFLP polymorphism and small sample-size subgroups (all P > 0.05). Our findings indicate that the MTP -493G/ T polymorphism may contribute to the development of NAFLD. Thus, the MTP -493G/T polymorphism may be a biomarker for the early detection of NAFLD.

  20. Integration of a wave rotor to an ultra-micro gas turbine (UmuGT)

    NASA Astrophysics Data System (ADS)

    Iancu, Florin

    2005-12-01

    Wave rotor technology has shown a significant potential for performance improvement of thermodynamic cycles. The wave rotor is an unsteady flow machine that utilizes shock waves to transfer energy from a high energy fluid to a low energy fluid, increasing both the temperature and the pressure of the low energy fluid. Used initially as a high pressure stage for a gas turbine locomotive engine, the wave rotor was commercialized only as a supercharging device for internal combustion engines, but recently there is a stronger research effort on implementing wave rotors as topping units or pressure gain combustors for gas turbines. At the same time, Ultra Micro Gas Turbines (UmuGT) are expected to be a next generation of power source for applications from propulsion to power generation, from aerospace industry to electronic industry. Starting in 1995, with the MIT "Micro Gas Turbine" project, the mechanical engineering research world has explored more and more the idea of "Power MEMS". Microfabricated turbomachinery like turbines, compressors, pumps, but also electric generators, heat exchangers, internal combustion engines and rocket engines have been on the focus list of researchers for the past 10 years. The reason is simple: the output power is proportional to the mass flow rate of the working fluid through the engine, or the cross-sectional area while the mass or volume of the engine is proportional to the cube of the characteristic length, thus the power density tends to increase at small scales (Power/Mass=L -1). This is the so-called "cube square law". This work investigates the possibilities of incorporating a wave rotor to an UmuGT and discusses the advantages of wave rotor as topping units for gas turbines, especially at microscale. Based on documented wave rotor efficiencies at larger scale and subsidized by both, a gasdynamic model that includes wall friction, and a CFD model, the wave rotor compression efficiency at microfabrication scale could be estimated

  1. DNMT3B 579G>T promoter polymorphism and the risk for idiopathic thrombocytopenic purpura in a Chinese population.

    PubMed

    Zhao, Haifeng; Du, Weiting; Gu, Dongsheng; Wang, Donghai; Xue, Feng; Ge, Jing; Sui, Tao; Yang, Renchi

    2009-01-01

    Epigenetics may influence the expression of numerous genes, which might contribute to autoimmune diseases. DNA methylation is mediated by DNA methyltransferases, especially DNA methyltransferase 3B (DNMT3B). Polymorphisms of the DNMT3B gene may influence DNMT3B activity on DNA methylation and increase the susceptibility to several diseases. The current study investigated the association between DNMT3B 579G>T and the risk for idiopathic thrombocytopenic purpura (ITP). The DNMT3B 579G>T polymorphisms were analyzed by PCR-RFLP. There was no significant difference in genotype and allele distribution between the ITP patient and the controls (p = 0.722 and 0.667, respectively). Similar results were observed between the 2 groups when stratified by age and disease course, including acute in childhood, chronic in childhood, acute in adult and chronic in adult. Importantly, this study showed a statistical difference in the distribution of SNP of DNMT3B between Chinese and Koreans or Americans. It is shown that the SNP of DNMT3B 579G>T may not be used on its own as a marker to predict the susceptibility to ITP in a Chinese population and that DNMT3B 579G>T promoter SNP varies from one ethnic population to another.

  2. GT microsatellite repeats in the heme oxygenase-1 gene promoter associated with abdominal aortic aneurysm in Croatian patients.

    PubMed

    Gregorek, Andrea Crkvenac; Gornik, Kristina Crkvenac; Polancec, Darija Stupin; Dabelic, Sanja

    2013-06-01

    Abdominal aortic aneurysm (AAA) is a complex genetic disorder caused by the interplay of genetic and environmental risk factors. The number of (GT)(n) repeats in the heme oxygenase-1 (HO-1) gene promoter modulates transcription of this enzyme, which might have anti-inflammatory, antioxidant, antiapoptotic, and antiproliferative effect. The distribution of alleles and genotypes in Croatian individuals genotyped for the (GT)(n) HO-1 polymorphism was similar to that in other European populations. Frequency of the short (S) alleles (GT < 25) was higher in AAA patients (41.9%) than in non-AAA individuals (28.2%, p = 0.0026) because there were more SL heterozygotes among the AAA patients. The SL genotype appeared to increase the risk for AAA, but the increase was not statistically significant after adjustment for age, sex, smoking, hypertension, and hyperlipidemia (OR = 1.53, 95% CI 0.90-3.09, p = 0.062). These findings contradict those of the only other study performed so far on the association of (GT)(n) HO-1 polymorphism and AAA.

  3. Validation of the Actigraph GT3X and ActivPAL Accelerometers for the Assessment of Sedentary Behavior

    ERIC Educational Resources Information Center

    Kim, Youngdeok; Barry, Vaughn W.; Kang, Minsoo

    2015-01-01

    This study examined (a) the validity of two accelerometers (ActiGraph GT3X [ActiGraph LLC, Pensacola, FL, USA] and activPAL [PAL Technologies Ltd., Glasgow, Scotland]) for the assessment of sedentary behavior; and (b) the variations in assessment accuracy by setting minimum sedentary bout durations against a proxy for direct observation using an…

  4. MTP -493G/T gene polymorphism is associated with steatosis in hepatitis C-infected patients.

    PubMed

    Siqueira, E R F; Oliveira, C P M S; Correa-Giannella, M L; Stefano, J T; Cavaleiro, A M; Fortes, M A H Z; Muniz, M T C; Silva, F S; Pereira, L M M B; Carrilho, F J

    2012-01-01

    The reduction of hepatic microsomal transfer protein (MTP) activity results in fatty liver, worsening hepatic steatosis and fibrosis in chronic hepatitis C (CHC). The G allele of the MTP gene promoter, -493G/T, has been associated with lower transcriptional activity than the T allele. We investigated this association with metabolic and histological variables in patients with CHC. A total of 174 untreated patients with CHC were genotyped for MTP -493G/T by direct sequencing using PCR. All patients were negative for markers of Wilson's disease, hemochromatosis and autoimmune diseases and had current and past daily alcohol intake lower than 100 g/week. The sample distribution was in Hardy-Weinberg equilibrium. Among subjects with genotype 1, 56.8% of the patients with fibrosis grade 3+4 presented at least one G allele versus 34.3% of the patients with fibrosis grade 1+2 (OR = 1.8; 95%CI = 1.3-2.3). Logistic regression analysis with steatosis as the dependent variable identified genotypes GG+GT as independent protective factors against steatosis (OR = 0.4, 95%CI = 0.2-0.8; P = 0.01). The results suggest that the presence of the G allele of MTP -493G/T associated with lower hepatic MTP expression protects against steatosis in our CHC patients.

  5. Susceptibility of GT1-7 cells to mouse-passaged field scrapie isolates with a long incubation.

    PubMed

    Miyazawa, Kohtaro; Okada, Hiroyuki; Iwamaru, Yoshifumi; Masujin, Kentaro; Yokoyama, Takashi

    2014-01-01

    A typical feature of scrapie in sheep and goats is the accumulation of disease-associated prion protein. Scrapie consists of many strains with different biological properties. Nine natural sheep scrapie cases were transmitted to wild-type mice and mouse-passaged isolates were classified into 2 types based on incubation time: short and long. These 2 types displayed a distinct difference in their pathology. We attempted to transmit these mouse-passaged isolates to 2 murine cell lines (GT1-7 and L929) to compare their properties. All of the isolates were transmitted to L929 cells. However, only mouse-passaged field isolates with a long incubation time were transmitted to GT1-7 cells. This specific susceptibility of GT1-7 cells was also confirmed with a primary-passaged isolate that was not completely adapted to the new host species. Characterization of the mechanisms of the specific susceptibility of GT1-7 cells to isolates with a long incubation time may lead to a greater understanding of the differences among prion strains.

  6. Genetic mapping and QTL analysis of disease resistance traits in peanut population Tifrunner x GT-C20

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A genetic map of peanut (Arachis hypogaea L.) with 426 SSR markers was constructed using a population of 162 recombinant inbred lines (RILs) from a cross between ‘Tifrunner’ and ‘GT-C20’. Linkage groups (LGs) were assigned to chromosomes using published peanut reference maps. The total length of the...

  7. The Arabidopsis Family GT43 Glycosyltransferases Form Two Functionally Nonredundant Groups Essential for the Elongation of Glucuronoxylan Backbone

    EPA Science Inventory

    There exist four members of family GT43 glycosyltransferases in the Arabidopsis (Arabidopsis thaliana) genome, and mutations of two of them, IRX9 and IRX14, have previously been shown to cause a defect in glucuronoxylan (GX) biosynthesis. However, it is currently unknown whether ...

  8. FEN1 −69G>A and +4150G>T polymorphisms and breast cancer risk

    PubMed Central

    Rezaei, Maryam; Hashemi, Mohammad; Sanaei, Sara; Mashhadi, Mohammad Ali; Hashemi, Seyed Mehdi; Bahari, Gholamreza; Taheri, Mohsen

    2016-01-01

    Flap endonuclease 1 (FEN1), a DNA repair protein, is important in preventing carcinogenesis. Two functional germ line variants −69G>A (rs174538) and +4150G>T (rs4246215) in the FEN1 gene have been associated with risk of various types of cancer. The aim of the present study was to evaluate the possible impact of FEN1 polymorphisms on risk of breast cancer (BC) in a sample of Iranian subjects. The FEN1 −69G>A and +4150G>T polymorphisms were analyzed in a case-control study that included 266 BC patients and 225 healthy females. Polymerase chain reaction-restriction fragment length polymorphism analysis was used to genotype the variants. The findings demonstrated that the FEN1 −69G>A and +4150G>T polymorphisms were not associated with BC risk in co-dominant, dominant and recessive inheritance models. The findings indicated that GG/GT, GA/GG and GA/TT genotypes significantly decreased the risk of BC when compared with −69GG/+4150GG. Furthermore, haplotype analysis indicated that −69G/+4150T as well as −69A/+4150G significantly decreased the risk of BC compared with −69G/+4150G. Thus, these findings demonstrated that haplotypes of FEN1 −69G>A and +4150G>T polymorphisms decreased the risk of BC in an Iranian population. Further studies with larger sample sizes and different ethnicities are required to validate the present findings. PMID:27699013

  9. Characterisation of Muta™Mouse λgt10-lacZ transgene: evidence for in vivo rearrangements

    PubMed Central

    Shwed, Philip S.; Crosthwait, Jennifer; Douglas, George R.; Seligy, Vern L.

    2010-01-01

    The multicopy λgt10-lacZ transgene shuttle vector of Muta™Mouse serves as an important tool for genotoxicity studies. Here, we describe a model for λgt10-lacZ transgene molecular structure, based on characterisation of transgenes recovered from animals of our intramural breeding colony. Unique nucleotide sequences of the 47 513 bp monomer are reported with GenBank® assigned accession numbers. Besides defining ancestral mutations of the λgt10 used to construct the transgene and the Muta™Mouse precursor (strain 40.6), we validated the sequence integrity of key λ genes needed for the Escherichia coli host-based mutation reporting assay. Using three polymerase chain reaction (PCR)-based chromosome scanning and cloning strategies, we found five distinct in vivo transgene rearrangements, which were common to both sexes, and involved copy fusions generating ∼10 defective copies per haplotype. The transgene haplotype was estimated by Southern hybridisation and real-time–polymerase chain reaction, which yielded 29.0 ± 4.0 copies based on spleen DNA of Muta™Mouse, and a reconstructed CD2F1 genome with variable λgt10-lacZ copies. Similar analysis of commercially prepared spleen DNA from Big Blue® mouse yielded a haplotype of 23.5 ± 3.1 copies. The latter DNA is used in calibrating a commercial in vitro packaging kit for E.coli host-based mutation assays of both transgenic systems. The model for λgt10-lacZ transgene organisation, and the PCR-based methods for assessing copy number, integrity and rearrangements, potentially extends the use of Muta™Mouse construct for direct, genomic-type assays that detect the effects of clastogens and aneugens, without depending on an E.coli host, for reporting effects. PMID:20724577

  10. The FSHB -211G>T variant attenuates serum FSH levels in the supraphysiological gonadotropin setting of Klinefelter syndrome.

    PubMed

    Busch, Alexander S; Tüttelmann, Frank; Zitzmann, Michael; Kliesch, Sabine; Gromoll, Jörg

    2015-05-01

    Klinefelter syndrome (47, XXY) is the most frequent genetic cause of male infertility and individuals share the endocrine hallmark of hypergonadotropic hypogonadism. Single-nucleotide polymorphisms located within the FSHB/FSHR gene were recently shown to impact serum follicle-stimulating hormone (FSH) levels and other reproductive parameters in men. The objective of this study was to analyse the effect of FSHB-211G>T (c.-280G>T, rs10835638) as well as FSHR c.2039G>A (rs6166) and FSHR c.-29G>A (rs1394205) on endocrine and reproductive parameters in untreated and testosterone-treated Klinefelter patients. Patients were retrospectively selected from the clientele attending a university-based andrology centre. A total of 309 non-mosaic Klinefelter individuals between 18 and 65 years were included and genotyped for the variants by TaqMan assays. The untreated group comprised 248 men, in which the FSHB -211G>T allele was significantly associated with the reduced serum follicle-stimulating hormone levels (-6.5 U/l per T allele, P=1.3 × 10(-3)). Testosterone treatment (n=150) abolished the observed association. When analysing patients before and under testosterone treatment (n=89), gonadotropin levels were similarly suppressed independently of the FSHB genotype. The FSHR polymorphisms did not exhibit any significant influence in any group, neither on the endocrine nor reproductive parameters. In conclusion, a hypergonadotropic setting such as Klinefelter syndrome does not mask the FSHB -211G>T genotype effects on the follicle-stimulating hormone serum levels. The impact was indeed more pronounced compared with normal or infertile men, whereas gonadotropin suppression under testosterone treatment seems to be independent of the genotype. Thus, the FSHB -211G>T genotype is a key determinant in the regulation of gonadotropins in different reproductive-endocrine pathopyhsiologies.

  11. Identification and in silico characterization of soybean trihelix-GT and bHLH transcription factors involved in stress responses

    PubMed Central

    Osorio, Marina Borges; Bücker-Neto, Lauro; Castilhos, Graciela; Turchetto-Zolet, Andreia Carina; Wiebke-Strohm, Beatriz; Bodanese-Zanettini, Maria Helena; Margis-Pinheiro, Márcia

    2012-01-01

    Environmental stresses caused by either abiotic or biotic factors greatly affect agriculture. As for soybean [Glycine max (L.) Merril], one of the most important crop species in the world, the situation is not different. In order to deal with these stresses, plants have evolved a variety of sophisticated molecular mechanisms, to which the transcriptional regulation of target-genes by transcription factors is crucial. Even though the involvement of several transcription factor families has been widely reported in stress response, there still is a lot to be uncovered, especially in soybean. Therefore, the objective of this study was to investigate the role of bHLH and trihelix-GT transcription factors in soybean responses to environmental stresses. Gene annotation, data mining for stress response, and phylogenetic analysis of members from both families are presented herein. At least 45 bHLH (from subgroup 25) and 63 trihelix-GT putative genes reside in the soybean genome. Among them, at least 14 bHLH and 11 trihelix-GT seem to be involved in responses to abiotic/biotic stresses. Phylogenetic analysis successfully clustered these with members from other plant species. Nevertheless, bHLH and trihelix-GT genes encompass almost three times more members in soybean than in Arabidopsis or rice, with many of these grouping into new clades with no apparent near orthologs in the other analyzed species. Our results represent an important step towards unraveling the functional roles of plant bHLH and trihelix-GT transcription factors in response to environmental cues. PMID:22802709

  12. Single-level optimization of a hybrid SOFC-GT power plant

    NASA Astrophysics Data System (ADS)

    Calise, F.; Dentice d'Accadia, M.; Vanoli, L.; von Spakovsky, M. R.

    The detailed synthesis/design optimization of a hybrid solid oxide fuel cell-gas turbine (SOFC-GT) power plant is presented in this paper. In the first part of the paper, the bulk-flow model used to simulate the plant is discussed. The performance of the centrifugal compressors and radial turbine is determined using maps, properly scaled in order to match the values required for mass flow rate and pressure ratio. Compact heat exchangers are simulated using Colburn and friction factor correlations. For the SOFC, the cell voltage versus current density curves (i.e. polarization curves) are generated on the basis of the Nernst potential and overvoltages. Validation of the SOFC polarization curves is accomplished with data available from Siemens Westinghouse. Both the steam-methane pre-reforming and internal reforming processes are modeled assuming the water-gas shift reaction to be equilibrium-controlled and the demethanization reactions to be kinetically controlled. Finally, a thermoeconomic model is developed by introducing capital cost functions for each plant component. The whole plant is first simulated for a fixed configuration. Then, a synthesis/design optimization of the plant is carried out using a traditional single-level approach. The results of the optimization are presented and discussed.

  13. Comparison of GT3X accelerometer and Yamax pedometer steps/day in a free-living sample of overweight and obese adults

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The purpose of this study was to compare steps/day detected by the YAMAX SW-200 pedometer versus the Actigraph GT3X accelerometer in free-living adults. Daily YAMAX and GT3X steps were collected from a sample of 23 overweight and obese participants (78% female; age = 52.6 +/- 8.4 yr.; BMI = 31.0 +/-...

  14. DOE/Allison ceramic vane effort

    SciTech Connect

    Wenglarz, R.; Browning, W.; Calcuttawala, S.

    1995-10-01

    An objective of the Advanced Turbine Systems (ATS) program is to develop ultra-high efficiency gas turbine systems. Rotor inlet temperatures several hundred degrees greater than for the highest temperature current industrial engines will be required to meet the ATS objectives. Consequently, new technologies need to be developed and demonstrated to achieve the required ultra-high ATS efficiencies.

  15. Allison engine ATS program technical review

    SciTech Connect

    Mukavetz, D.

    1995-10-01

    Gas turbines in industrial and utility applications can help meet future national and worldwide power generation requirements. Turbine systems burning natural gas offer environmentally sound and economical power generation and cogeneration. Since U.S. demand alone will require up to 15 gigawatts per year of new and replacement capacity after the year 2000, the availability of Advanced Turbine Systems (ATS) to fill a share of this need will save significant amounts of fuel and benefit the environment. Implementation of the ATS Program will also keep U.S. manufacturers on the cutting ledge of turbine technology for power generation applications and enhance the nation`s economic competitiveness.

  16. Unique motifs identify PIG-A proteins from glycosyltransferases of the GT4 family

    PubMed Central

    2008-01-01

    Background The first step of GPI anchor biosynthesis is catalyzed by PIG-A, an enzyme that transfers N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol. This protein is present in all eukaryotic organisms ranging from protozoa to higher mammals, as part of a larger complex of five to six 'accessory' proteins whose individual roles in the glycosyltransferase reaction are as yet unclear. The PIG-A gene has been shown to be an essential gene in various eukaryotes. In humans, mutations in the protein have been associated with paroxysomal noctural hemoglobuinuria. The corresponding PIG-A gene has also been recently identified in the genome of many archaeabacteria although genes of the accessory proteins have not been discovered in them. The present study explores the evolution of PIG-A and the phylogenetic relationship between this protein and other glycosyltransferases. Results In this paper we show that out of the twelve conserved motifs identified by us eleven are exclusively present in PIG-A and, therefore, can be used as markers to identify PIG-A from newly sequenced genomes. Three of these motifs are absent in the primitive eukaryote, G. lamblia. Sequence analyses show that seven of these conserved motifs are present in prokaryote and archaeal counterparts in rudimentary forms and can be used to differentiate PIG-A proteins from glycosyltransferases. Using partial least square regression analysis and data involving presence or absence of motifs in a range of PIG-A and glycosyltransferases we show that (i) PIG-A may have evolved from prokaryotic glycosyltransferases and lipopolysaccharide synthases, members of the GT4 family of glycosyltransferases and (ii) it is possible to uniquely classify PIG-A proteins versus glycosyltransferases. Conclusion Besides identifying unique motifs and showing that PIG-A protein from G. lamblia and some putative PIG-A proteins from archaebacteria are evolutionarily closer to glycosyltransferases, these studies

  17. Draft Genome Sequence of Kluyvera intestini Strain GT-16 Isolated from the Stomach of a Patient with Gastric Cancer

    PubMed Central

    Tetz, Victor

    2016-01-01

    Here, we report the complete genome sequence of the novel, non-spore-forming Kluyvera intestini strain GT-16, isolated from the stomach of a patient with gastric cancer. The genome is 5,868,299 bp in length with a G+C content of 53.0%. It possesses 5,350 predicted protein-coding genes encoding virulence factors and antibiotic resistance proteins. PMID:28007864

  18. Scrapie protein degradation by cysteine proteases in CD11c+ dendritic cells and GT1-1 neuronal cells.

    PubMed

    Luhr, Katarina M; Nordström, Elin K; Löw, Peter; Ljunggren, Hans-Gustaf; Taraboulos, Albert; Kristensson, Krister

    2004-05-01

    Dendritic cells (DC) of the CD11c(+) myeloid phenotype have been implicated in the spread of scrapie in the host. Previously, we have shown that CD11c(+) DC can cause a rapid degradation of proteinase K-resistant prion proteins (PrP(Sc)) in vitro, indicating a possible role of these cells in the clearance of PrP(Sc). To determine the mechanisms of PrP(Sc) degradation, CD11c(+) DC that had been exposed to PrP(Sc) derived from a neuronal cell line (GT1-1) infected with scrapie (ScGT1-1) were treated with a battery of protease inhibitors. Following treatment with the cysteine protease inhibitors (2S,3S)-trans-epoxysuccinyl-L-leucylamido-3-methylbutane (E-64c), its ethyl ester (E-64d), and leupeptin, the degradation of PrP(Sc) was inhibited, while inhibitors of serine and aspartic and metalloproteases (aprotinin, pepstatin, and phosphoramidon) had no effect. An endogenous degradation of PrP(Sc) in ScGT1-1 cells was revealed by inhibiting the expression of cellular PrP (PrP(C)) by RNA interference, and this degradation could also be inhibited by the cysteine protease inhibitors. Our data show that PrP(Sc) is proteolytically cleaved preferentially by cysteine proteases in both CD11c(+) DC and ScGT1-1 cells and that the degradation of PrP(Sc) by proteases is different from that of PrP(C). Interference by protease inhibitors with DC-induced processing of PrP(Sc) has the potential to modify prion spread, clearance, and immunization in a host.

  19. Helicobacter hepaticus Hh0072 gene encodes a novel alpha1-3-fucosyltransferase belonging to CAZy GT11 family.

    PubMed

    Zhang, Lei; Lau, Kam; Cheng, Jiansong; Yu, Hai; Li, Yanhong; Sugiarto, Go; Huang, Shengshu; Ding, Li; Thon, Vireak; Wang, Peng G; Chen, Xi

    2010-09-01

    Lewis x (Le(x)) and sialyl Lewis x (SLe(x))-containing glycans play important roles in numerous physiological and pathological processes. The key enzyme for the final step formation of these Lewis antigens is alpha1-3-fucosyltransferase. Here we report molecular cloning and functional expression of a novel Helicobacter hepaticus alpha1-3-fucosyltransferase (HhFT1) which shows activity towards both non-sialylated and sialylated Type II oligosaccharide acceptor substrates. It is a promising catalyst for enzymatic and chemoenzymatic synthesis of Le(x), sialyl Le(x) and their derivatives. Unlike all other alpha1-3/4-fucosyltransferases characterized so far which belong to Carbohydrate Active Enzyme (CAZy, http://www.cazy.org/) glycosyltransferase family GT10, the HhFT1 shares protein sequence homology with alpha1-2-fucosyltransferases and belongs to CAZy glycosyltransferase family GT11. The HhFT1 is thus the first alpha1-3-fucosyltransferase identified in the GT11 family.

  20. Thermo-economic comparative analysis of gas turbine GT10 integrated with air and steam bottoming cycle

    NASA Astrophysics Data System (ADS)

    Czaja, Daniel; Chmielnak, Tadeusz; Lepszy, Sebastian

    2014-12-01

    A thermodynamic and economic analysis of a GT10 gas turbine integrated with the air bottoming cycle is presented. The results are compared to commercially available combined cycle power plants based on the same gas turbine. The systems under analysis have a better chance of competing with steam bottoming cycle configurations in a small range of the power output capacity. The aim of the calculations is to determine the final cost of electricity generated by the gas turbine air bottoming cycle based on a 25 MW GT10 gas turbine with the exhaust gas mass flow rate of about 80 kg/s. The article shows the results of thermodynamic optimization of the selection of the technological structure of gas turbine air bottoming cycle and of a comparative economic analysis. Quantities are determined that have a decisive impact on the considered units profitability and competitiveness compared to the popular technology based on the steam bottoming cycle. The ultimate quantity that can be compared in the calculations is the cost of 1 MWh of electricity. It should be noted that the systems analyzed herein are power plants where electricity is the only generated product. The performed calculations do not take account of any other (potential) revenues from the sale of energy origin certificates. Keywords: Gas turbine air bottoming cycle, Air bottoming cycle, Gas turbine, GT10

  1. Reduction of Hexavalent Chromium by Green Tea Polyphenols and Green Tea Nano Zero-Valent Iron (GT-nZVI).

    PubMed

    Chrysochoou, M; Reeves, K

    2017-03-01

    This study reports on the direct reduction of hexavalent chromium [Cr(VI)] by green tea polyphenols, including a green tea solution and pure epigallocatechin gallate (EGCG) solution. A linear trend was observed between the amount of reduced Cr(VI) and the amount of added polyphenols. The green tea solution showed a continued decrease in the observed stoichiometry with increasing pH, from a maximum of 1.4 mol per gallic acid equivalent (GAE) of green tea at pH 2.5, to 0.2 mol/GAE at pH 8.8. The EGCG solution exhibited different behavior, with a maximum stoichiometry of 2 at pH 7 and minimum of 1.6 at pH 4.4 and 8.9. When green tea was used to first react with Fe(3+) and form GT-nZVI, the amount of Cr(VI) reduced by a certain volume of GT-nZVI was double compared to green tea, and 6 times as high considering that GT-nZVI only contains 33 % green tea.

  2. Experimental study of cesium 5D+5D-&gt;6S+(nL=9D,11S,7F) energy pooling collisions

    NASA Astrophysics Data System (ADS)

    Wang, Qian; Dai, Kang; Shen, Yifan

    2007-07-01

    We report experimentally the measured rate coefficients for the energy pooling (EP) collisions process Cs(5D)+Cs(5D)-&gt;Cs(6S)+Cs(nL=9D,11S,7F) in cesium densities of 10^(16)-10^(17) cm^(-3). The 5D state was populated via 8S-&gt;7P-&gt;5D spontaneous emission following two-step pumping 6S-&gt;6P_(3/2)-&gt;8S. Since the 5D-&gt;6P (3.0-3.6 microns) fluorescence could not be detected in this experiment, we carried out a relative measurement for the process 6P+5D-&gt;6S+7D. The excited-atom density and spatial distribution were mapped by monitoring the absorption of a counterpropagating single-mode laser beam, tuned to 6P_(3/2)-&gt;9S_(1/2) transition, which could be translated parallelly to the pump beam. The excited atom densities have been combined with the measured fluorescence ratios to yield EP rate coefficients. The average values for nL=9D,11S and 7F are 8.0+-4.0, 7.0+-3.5, and 9.3+-4.6 (in units of 10^(-10) cm3/s), respectively. Influence of the energy transfer process 11S+6S-&gt;7F+6S on the rate coefficients k_(11S) and k_(7F) is also discussed.

  3. Regulation of GNRH production by estrogen and bone morphogenetic proteins in GT1-7 hypothalamic cells.

    PubMed

    Otani, Hiroyuki; Otsuka, Fumio; Takeda, Masaya; Mukai, Tomoyuki; Terasaka, Tomohiro; Miyoshi, Tomoko; Inagaki, Kenichi; Suzuki, Jiro; Ogura, Toshio; Lawson, Mark A; Makino, Hirofumi

    2009-10-01

    Recent studies have shown that bone morphogenetic proteins (BMPs) are important regulators in the pituitary-gonadal endocrine axis. We here investigated the effects of BMPs on GNRH production controlled by estrogen using murine GT1-7 hypothalamic neuron cells. GT1-7 cells expressed estrogen receptor alpha (ERalpha; ESR1 as listed in MGI Database), ERbeta (ESR2 as listed in MGI Database), BMP receptors, SMADs, and a binding protein follistatin. Treatment with BMP2 and BMP4 had no effect on Gnrh mRNA expression; however, BMP6 and BMP7 significantly increased Gnrh mRNA expression as well as GnRH production by GT1-7 cells. Notably, the reduction of Gnrh expression caused by estradiol (E(2)) was restored by cotreatment with BMP2 and BMP4, whereas it was not affected by BMP6 or BMP7. E(2) activated extracellular signal-regulated kinase (ERK) 1/2 and stress-activated protein kinase/c-Jun NH(2)-terminal kinase (SAPK/JNK) signaling but did not activate p38-mitogen-activated protein kinase (MAPK) signaling in GT1-7 cells. Inhibition of ERK1/ERK2 reversed the inhibitory effect of estrogen on Gnrh expression, whereas SAPK/JNK inhibition did not affect the E(2) actions. Expression levels of Eralpha and Erbeta were reduced by BMP2 and BMP4, but were increased by BMP6 and BMP7. Treatment with an ER antagonist inhibited the E(2) effects on Gnrh suppression including reduction of E(2)-induced ERK phosphorylation, suggesting the involvement of genomic ER actions in Gnrh suppression. BMP2 and BMP4 also suppressed estrogen-induced phosphorylation of ERK1/ERK2 and SAPK/JNK signaling, suggesting that BMP2 and BMP4 downregulate estrogen effects by attenuating ER-MAPK signaling. Considering that BMP6 and BMP7 increased the expression of alpha1E-subunit of R-type calcium channel (Cacna1e), which is critical for GNRH secretion, it is possible that BMP6 and BMP7 directly stimulate GNRH release by GT1-7 cells. Collectively, a newly uncovered interaction of BMPs and ER may be involved in

  4. GT-MSOCC - A domain for research on human-computer interaction and decision aiding in supervisory control systems. [Georgia Tech - Multisatellite Operations Control Center

    NASA Technical Reports Server (NTRS)

    Mitchell, Christine M.

    1987-01-01

    The Georgia Tech-Multisatellite Operations Control Center (GT-MSOCC), a real-time interactive simulation of the operator interface to a NASA ground control system for unmanned earth-orbiting satellites, is described. The GT-MSOCC program for investigating a range of modeling, decision aiding, and workstation design issues related to the human-computer interaction is discussed. A GT-MSOCC operator function model is described in which operator actions, both cognitive and manual, are represented as the lowest level discrete control network nodes, and operator action nodes are linked to information needs or system reconfiguration commands.

  5. Genotyping-in-Thousands by sequencing (GT-seq): A cost effective SNP genotyping method based on custom amplicon sequencing.

    PubMed

    Campbell, Nathan R; Harmon, Stephanie A; Narum, Shawn R

    2015-07-01

    Genotyping-in-Thousands by sequencing (GT-seq) is a method that uses next-generation sequencing of multiplexed PCR products to generate genotypes from relatively small panels (50-500) of targeted single-nucleotide polymorphisms (SNPs) for thousands of individuals in a single Illumina HiSeq lane. This method uses only unlabelled oligos and PCR master mix in two thermal cycling steps for amplification of targeted SNP loci. During this process, sequencing adapters and dual barcode sequence tags are incorporated into the amplicons enabling thousands of individuals to be pooled into a single sequencing library. Post sequencing, reads from individual samples are split into individual files using their unique combination of barcode sequences. Genotyping is performed with a simple perl script which counts amplicon-specific sequences for each allele, and allele ratios are used to determine the genotypes. We demonstrate this technique by genotyping 2068 individual steelhead trout (Oncorhynchus mykiss) samples with a set of 192 SNP markers in a single library sequenced in a single Illumina HiSeq lane. Genotype data were 99.9% concordant to previously collected TaqMan(™) genotypes at the same 192 loci, but call rates were slightly lower with GT-seq (96.4%) relative to Taqman (99.0%). Of the 192 SNPs, 187 were genotyped in ≥90% of the individual samples and only 3 SNPs were genotyped in <70% of samples. This study demonstrates amplicon sequencing with GT-seq greatly reduces the cost of genotyping hundreds of targeted SNPs relative to existing methods by utilizing a simple library preparation method and massive efficiency of scale.

  6. [The balanced force and the GT-rotary technique in comparison with the non-instrumental technique (NIT)].

    PubMed

    Lussi, Adrian; Hotz, Meret; Stich, Hermann

    2004-01-01

    The aim of the study was to compare the cleansing effect of the latest modification of the non-instrumentation technique (NIT) to that of conventional instrumentation. The root curvature in 100 vital human molars was determined by a standardized X-ray procedure and the teeth were assigned to five groups with 20 teeth each with an equal distribution of the root curvature. The preparation methods were the Balanced Force technique and the GT Rotary technique. Each root was irrigated with 40 ml of 3% sodium hypochlorite. The other groups were irrigated by NIT during 2.5, 5 or 10 minutes, respectively. The remaining pulpal tissue was stained and the root canals were exposed longitudinally. The teeth were then evaluated using a microscope and an image analysis-system. The residual organic debris in the apical, middle and coronal sections of the root canals were assessed as a percentage of the corresponding total examined length. The cleansing effect of the NIT in the coronal and middle parts of the canal used for 5 and 10 minutes was significantly better (p < 0.05) compared to using the device for 2.5 minutes. The cleansing effect of the NIT in the coronal and middle parts of the canal used for 5 and 10 minutes was also significantly better (p < 0.05) compared to using the GT Rotary or Balanced Force techniques. Apically, the cleansing effect of the NIT used for 5 and 10 minutes and the GT Rotary technique was significantly better (p < 0.05) compared to using the Balanced Force technique or the NIT for 2.5 minutes. It was concluded that the cleansing effect of the latest modification of the Non-instrumentation Technology (NIT) was equivalent to or better than that of conventional instrumentation requiring significantly less time.

  7. Hypertriglyceridemia associated with the c.553G>T APOA5 SNP results from aberrant hetero-disulfide bond formation

    PubMed Central

    Sharma, Vineeta; Witkowski, Andrzej; Witkowska, H. Ewa; Dykstra, Andrew; Simonsen, Jens B.; Nelbach, Lisa; Beckstead, Jennifer A.; Pullinger, Clive R.; Kane, John P.; Malloy, Mary J.; Watson, Gordon; Forte, Trudy M.; Ryan, Robert O.

    2014-01-01

    Objective Apolipoprotein (apo) A-V is a low abundance plasma protein that modulates triacylglycerol (TG) homeostasis. Gene transfer studies were undertaken in apoa5 (−/−) mice to define the mechanism underlying the correlation between the single nucleotide polymorphism (SNP) c.553G>T in APOA5 and hypertriglyceridemia (HTG). Approach and Results Adeno-associated virus (AAV) 2/8 mediated gene transfer of wild type (WT) apoA-V induced a dramatic lowering of plasma TG in apoa5 (−/−) mice while AAV2/8-Gly162Cys apoA-V (corresponding to the c.553G>T SNP: rs2075291) had a modest effect. Characterization studies revealed that plasma levels of WT- and G162C apoA-V in transduced mice were similar and within the physiological range. Fractionation of plasma from mice transduced with AAV2/8-G162C apoA-V indicated that, unlike WT apoA-V, >50% of G162C apoA-V was recovered in the lipoprotein-free fraction. Non-reducing SDS-PAGE immunoblot analysis provided evidence that G162C apoA-V present in the lipoprotein-free fraction, but not that portion associated with lipoproteins, displayed altered electrophoretic mobility consistent with disulfide-linked hetero-dimer formation. Immunoprecipitation followed by liquid chromatography/mass spectrometry of human plasma from subjects homozygous for WT APOA5 and c.553G>T APOA5 revealed that G162C apoA-V forms adducts with extraneous plasma proteins including fibronectin, kininogen-1 and others. Conclusion Substitution of Cys for Gly at position 162 of mature apoA-V introduces a free cysteine that forms disulfide bonds with plasma proteins such that its lipoprotein binding and TG modulation functions are compromised. PMID:25127531

  8. Operational, control and protective system transient analyses of the closed-cycle GT-HTGR power plant

    SciTech Connect

    Openshaw, F.L.; Chan, T.W.

    1980-07-01

    This paper presents a description of the analyses of the control/protective system preliminary designs for the gas turbine high-temperature gas-cooled reactor (GT-HTGR) power plant. The control system is designed to regulate reactor power, control electric load and turbine speed, control the temperature of the helium delivered to the turbines, and control thermal transients experienced by the system components. In addition, it provides the required control programming for startup, shutdown, load ramp, and other expected operations. The control system also handles conditions imposed on the system during upset and emergency conditions such as loop trip, reactor trip, or electrical load rejection.

  9. Evaluation of Cathode Air Flow Transients in a SOFC/GT Hybrid System Using Hardware in the Loop Simulation.

    PubMed

    Zhou, Nana; Yang, Chen; Tucker, David

    2015-02-01

    Thermal management in the fuel cell component of a direct fired solid oxide fuel cell gas turbine (SOFC/GT) hybrid power system can be improved by effective management and control of the cathode airflow. The disturbances of the cathode airflow were accomplished by diverting air around the fuel cell system through the manipulation of a hot-air bypass valve in open loop experiments, using a hardware-based simulation facility designed and built by the U.S. Department of Energy, National Energy Technology Laboratory (NETL). The dynamic responses of the fuel cell component and hardware component of the hybrid system were studied in this paper.

  10. Computational Assessment of the GT-MHR Graphite Core Support Structural Integrity in Air-Ingress Accident Condition

    SciTech Connect

    Jong B. Lim; Eung S. Kim; Chang H. Oh; Richard R. Schultz; David A. Petti

    2008-10-01

    The objective of this project was to perform stress analysis for graphite support structures of the General Atomics’ 600 MWth GT-MHR prismatic core design using ABAQUS ® (ver. 6.75) to assess their structural integrity in air-ingress accident conditions where the structure weakens over time due to oxidation damages. The graphite support structures of prismatic type GT-MHR was analyzed based on the change of temperature, burn-off and corrosion depth during the accident period predicted by GAMMA, a multi-dimensional gas multi-component mixture analysis code developed in the Republic of Korea (ROK)/United States (US) International –Nuclear Engineering Research Initiative (I-NERI) project. Both the loading and thermal stresses were analyzed, but the thermal stress was not significant, leaving the loading stress to be the major factor. The mechanical strengths are exceeded between 11 to 11.5 days after loss-of-coolant-accident (LOCA), corresponding to 5.5 to 6 days after the start of natural convection.

  11. Human OCT2 variant c.808G>T confers protection effect against cisplatin-induced ototoxicity

    PubMed Central

    Lanvers-Kaminsky, Claudia; Sprowl, Jason A; Malath, Ingrid; Deuster, Dirk; Eveslage, Maria; Schlatter, Eberhard; Mathijssen, Ron HJ; Boos, Joachim; Jürgens, Heribert; am Zehnhoff-Dinnesen, Antionette G; Sparreboom, Alex; Ciarimboli, Giuliano

    2016-01-01

    Aim Assuming that genetic variants of the SLC22A2 and SLC31A1 transporter affect patients’ susceptibility to cisplatin-induced ototoxicity, we compared the distribution of 11 SLC22A2 variants and the SLC31A1 variant rs10981694 between patients with and without cisplatin-induced ototoxicity. Patients & methods Genotyping was performed in 64 pediatric patients and significant findings were re-evaluated in 66 adults. Results The SLC22A2 polymorphism rs316019 (c.808G>T; Ser270Ala) was significantly associated with protection from cisplatin-induced ototoxicity in the pediatric (p = 0.022) and the adult cohort (p = 0.048; both: Fisher’s exact test). This result was confirmed by multiple logistic regression analysis accounting for age which was identified as a relevant factor for ototoxicity as well (rs316019: OR [G/T vs G/G] = 0.12, p = 0.009; age: OR [per year]: 0.84, p = 0.02). Conclusion These results identified rs316019 as potential pharmacogenomic marker for cisplatin-induced ototoxicity and point to a critical role of SLC22A2 for cisplatin transport in humans and its contribution to the organ specific side effects of this drug. PMID:25823781

  12. Analysis of systematic errors of the ASM/RXTE monitor and GT-48 γ-ray telescope

    NASA Astrophysics Data System (ADS)

    Fidelis, V. V.

    2011-06-01

    The observational data concerning variations of light curves of supernovae remnants—the Crab Nebula, Cassiopeia A, Tycho Brahe, and pulsar Vela—over 14 days scale that may be attributed to systematic errors of the ASM/RXTE monitor are presented. The experimental systematic errors of the GT-48 γ-ray telescope in the mono mode of operation were also determined. For this the observational data of TeV J2032 + 4130 (Cyg γ-2, according to the Crimean version) were used and the stationary nature of its γ-ray emission was confirmed by long-term observations performed with HEGRA and MAGIC. The results of research allow us to draw the following conclusions: (1) light curves of supernovae remnants averaged for long observing periods have false statistically significant flux variations, (2) the level of systematic errors is proportional to the registered flux and decreases with increasing temporal scale of averaging, (3) the light curves of sources may be modulated by the year period, and (4) the systematic errors of the GT-48 γ-ray telescope, in the amount caused by observations in the mono mode and data processing with the stereo-algorithm come to 0.12 min-1.

  13. A novel Tetra-primer ARMS-PCR based assay for genotyping SNP rs12303764(G/T) of human Unc-51 like kinase 1 gene.

    PubMed

    Randhawa, Rohit; Duseja, Ajay; Changotra, Harish

    2017-02-01

    Various case-control studies have shown association of single nucleotide polymorphism rs12303764(G/T) in ULK1 with crohn's disease. The techniques used in these studies were time consuming, complicated and require sophisticated/expensive instruments. Therefore, in order to overcome these problems, we have developed a new, rapid and cost effective Tetra-primer ARMS-PCR assay to genotype single nucleotide polymorphism rs12303764(G/T) of ULK1 gene. We manually designed allele specific primers. DNA fragment amplified using outer primers was sequenced to obtain samples with known genotypes (GG, GT and TT) for further use in the development of T-ARMS-PCR assay. Amplification conditions were optimized for parameters; annealing temperature, Taq DNA polymerase and primers. The developed T-ARMS-PCR assay was applied to genotype one hundred samples from healthy individuals. Genotyping results of 10 DNA samples from healthy individuals for rs12303764(G/T) by T-ARMS-PCR assay and sequencing were concordant. The newly developed assay was further applied to genotype samples from 100 healthy individuals of North Indian origin. Genotype frequencies were 9, 34 and 57 % for GG, GT and TT, respectively. Allele frequencies were 0.26 and 0.74 for G and T, respectively. The allele frequencies were in Hardy-Weinberg's equilibrium (p = 0.2443). T-ARMS-PCR assay developed in our laboratory for genotyping rs12303764 (G/T) of ULK1 gene is time saving and cost-effective as compared to the available methods. Furthermore, this is the first study reporting allelic and genotype frequencies of ULK1 rs12303764 (G/T) variants in North Indian population.

  14. Chloride conducting light activated channel GtACR2 can produce both cessation of firing and generation of action potentials in cortical neurons in response to light.

    PubMed

    Malyshev, A Y; Roshchin, M V; Smirnova, G R; Dolgikh, D A; Balaban, P M; Ostrovsky, M A

    2017-02-15

    Optogenetics is a powerful technique in neuroscience that provided a great success in studying the brain functions during the last decade. Progress of optogenetics crucially depends on development of new molecular tools. Light-activated cation-conducting channelrhodopsin2 was widely used for excitation of cells since the emergence of optogenetics. In 2015 a family of natural light activated chloride channels GtACR was identified which appeared to be a very promising tool for using in optogenetics experiments as a cell silencer. Here we examined properties of GtACR2 channel expressed in the rat layer 2/3 pyramidal neurons by means of in utero electroporation. We have found that despite strong inhibition the light stimulation of GtACR2-positive neurons can surprisingly lead to generation of action potentials, presumably initiated in the axonal terminals. Thus, when using the GtACR2 in optogenetics experiments, its ability to induce action potentials should be taken into account. Our results also open an interesting possibility of using the GtACR2 both as cell silencer and cell activator in the same experiment varying the pattern of light stimulation.

  15. Lentivirus-mediated RNAi knockdown of LMP2A inhibits the growth of the Epstein-Barr-associated gastric carcinoma cell line GT38 in vitro

    PubMed Central

    Wang, Fangjun; Chen, Weichang; Liu, Pengfei; Zhou, Jundong; Liu, Bingtuan; Ye, Wu; Wang, Wenping; Shen, Xiuyun

    2017-01-01

    In this study, lentivirus-mediated RNA interference (RNAi) was applied to inhibit latent membrane protein 2A (LMP2A) gene expression, in order to explore the effects of LMP2A silencing on the growth of an Epstein-Barr virus-associated gastric carcinoma (EBVaGC) cell line in vitro. Lentivirus-mediated RNAi technology was employed to specifically knock down the LMP2A gene in the EBV-positive gastric carcinoma cell line GT38. After infection, reverse transcription-quantitative polymerase chain reaction, western blotting, flow cytometry and colony formation assays were conducted to evaluate the expression of LMP2A and the biological behavior of the GT38 cell line in vitro. The results showed that the expression of the LMP2A gene was clearly downregulated in the infected cells, which indicated that a highly efficient and stable lentivirus vector was successfully constructed. In the GT38 cells in which the expression of LMP2A was downregulated, the proliferation and colony formation of the cells was significantly inhibited. In addition, it was found that the cell cycle of the GT38 cells was arrested in the G0/G1 phase and the apoptosis rate was increased. These results indicate that lentivirus-mediated RNAi knockdown of LMP2A inhibits the growth of the EBVaGC cell line GT38 in vitro, and suggests that LMP2A is a potential target for gene therapy in the treatment of EBVaGC. PMID:28123488

  16. Crystal Structure of Botulinum Neurotoxin Type a in Complex With the Cell Surface Co-Receptor GT1b-Insight Into the Toxin-Neuron Interaction

    SciTech Connect

    Stenmark, P.; Dupuy, J.; Inamura, A.; Kiso, M.; Stevens, R.C.

    2009-05-26

    Botulinum neurotoxins have a very high affinity and specificity for their target cells requiring two different co-receptors located on the neuronal cell surface. Different toxin serotypes have different protein receptors; yet, most share a common ganglioside co-receptor, GT1b. We determined the crystal structure of the botulinum neurotoxin serotype A binding domain (residues 873-1297) alone and in complex with a GT1b analog at 1.7 A and 1.6 A, respectively. The ganglioside GT1b forms several key hydrogen bonds to conserved residues and binds in a shallow groove lined by Tryptophan 1266. GT1b binding does not induce any large structural changes in the toxin; therefore, it is unlikely that allosteric effects play a major role in the dual receptor recognition. Together with the previously published structures of botulinum neurotoxin serotype B in complex with its protein co-receptor, we can now generate a detailed model of botulinum neurotoxin's interaction with the neuronal cell surface. The two branches of the GT1b polysaccharide, together with the protein receptor site, impose strict geometric constraints on the mode of interaction with the membrane surface and strongly support a model where one end of the 100 A long translocation domain helix bundle swing into contact with the membrane, initiating the membrane anchoring event.

  17. GT0 Explosion Sources for IMS Infrasound Calibration: Charge Design and Yield Estimation from Near-source Observations

    NASA Astrophysics Data System (ADS)

    Gitterman, Y.; Hofstetter, R.

    2014-03-01

    Three large-scale on-surface explosions were conducted by the Geophysical Institute of Israel (GII) at the Sayarim Military Range, Negev desert, Israel: about 82 tons of strong high explosives in August 2009, and two explosions of about 10 and 100 tons of ANFO explosives in January 2011. It was a collaborative effort between Israel, CTBTO, USA and several European countries, with the main goal to provide fully controlled ground truth (GT0) infrasound sources, monitored by extensive observations, for calibration of International Monitoring System (IMS) infrasound stations in Europe, Middle East and Asia. In all shots, the explosives were assembled like a pyramid/hemisphere on dry desert alluvium, with a complicated explosion design, different from the ideal homogenous hemisphere used in similar experiments in the past. Strong boosters and an upward charge detonation scheme were applied to provide more energy radiated to the atmosphere. Under these conditions the evaluation of the actual explosion yield, an important source parameter, is crucial for the GT0 calibration experiment. Audio-visual, air-shock and acoustic records were utilized for interpretation of observed unique blast effects, and for determination of blast wave parameters suited for yield estimation and the associated relationships. High-pressure gauges were deployed at 100-600 m to record air-blast properties, evaluate the efficiency of the charge design and energy generation, and provide a reliable estimation of the charge yield. The yield estimators, based on empirical scaled relations for well-known basic air-blast parameters—the peak pressure, impulse and positive phase duration, as well as on the crater dimensions and seismic magnitudes, were analyzed. A novel empirical scaled relationship for the little-known secondary shock delay was developed, consistent for broad ranges of ANFO charges and distances, which facilitates using this stable and reliable air-blast parameter as a new potential

  18. 36 CFR 404.4 - Access to information.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... life or physical safety of an individual; (ii) An urgency to inform the public about an actual or... than 10 days (excepting Saturdays, Sundays, or legal public holidays). Extensions may be granted by the FOIA Officer. The extension period may be split between the initial request and the appeal but in...

  19. 48 CFR 915.404-4-71-5 - Fee schedules.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... fees are related to the estimated cost (fee base) for the construction work and services to be... the fee base. (c) The fee schedule shown in paragraphs (d) and (f) of this section assumes a letter of... amount as determined from the schedules. (d) The following schedule sets forth the base for...

  20. GT2_proyer_3: Unveiling the evolutionary paths of the most massive stars through the study of their ejected nebulae

    NASA Astrophysics Data System (ADS)

    Royer, P.

    2011-05-01

    Several important questions remain open regarding the latest stages of evolution of the most massive stars, in particular regarding the exact evolutionary paths between the various subtypes of O stars, LBVs and Wolf-Rayet stars, and the mass-loss history of these objects throughout their lives. In the framework of the MESS GTKP+GT1, we have obtained or will obtain PACS imaging of 9 massive star nebulae of various types (LBV, LBV candidate, OF/WN, Of?p, WR) and PACS spectroscopy of 4 of them. In this short follow-up proposal we want to obtain PACS line spectroscopy for 3 peculiar massive and evolved objects for which spectroscopy is lacking. In particular, these observations will allow to determine the elemental abundances in the nebulae as well as the mass of the neutral gas using the fine structure lines formed in the ionized gas and in the photo-dissociation region respectively.

  1. Detailed Characterization of AR Coatings on Si Solar Cells: A New Application of GT-FabScan 6000; Preprint

    SciTech Connect

    Sopori, B.; Butterfield, B.; Amieva, J.

    2004-08-01

    We have developed a new application of GT-FabScan for rapid mapping of AR coatings on Si solar cells. The system generates an image of the AR thickness and presents it in a color format using false colors. This measurement is made in less than 100 ms. The development of this application enables the system to generate thickness maps of the AR coating to determine the repeatability of the deposition system, as well as to ensure that downstream processing can be controlled. These data can also be used to determine the average thickness of the coating. Downstream processing is an important issue in current solar cell technology. This paper describes its importance to the PV industry and discusses the principles and method of this measurement.

  2. Chronic exposure to KATP channel openers results in attenuated glucose sensing in hypothalamic GT1-7 neurons.

    PubMed

    Haythorne, Elizabeth; Hamilton, D Lee; Findlay, John A; Beall, Craig; McCrimmon, Rory J; Ashford, Michael L J

    2016-12-01

    Individuals with Type 1 diabetes (T1D) are often exposed to recurrent episodes of hypoglycaemia. This reduces hormonal and behavioural responses that normally counteract low glucose in order to maintain glucose homeostasis, with altered responsiveness of glucose sensing hypothalamic neurons implicated. Although the molecular mechanisms are unknown, pharmacological studies implicate hypothalamic ATP-sensitive potassium channel (KATP) activity, with KATP openers (KCOs) amplifying, through cell hyperpolarization, the response to hypoglycaemia. Although initial findings, using acute hypothalamic KCO delivery, in rats were promising, chronic exposure to the KCO NN414 worsened the responses to subsequent hypoglycaemic challenge. To investigate this further we used GT1-7 cells to explore how NN414 affected glucose-sensing behaviour, the metabolic response of cells to hypoglycaemia and KATP activity. GT1-7 cells exposed to 3 or 24 h NN414 exhibited an attenuated hyperpolarization to subsequent hypoglycaemic challenge or NN414, which correlated with diminished KATP activity. The reduced sensitivity to hypoglycaemia was apparent 24 h after NN414 removal, even though intrinsic KATP activity recovered. The NN414-modified glucose responsiveness was not associated with adaptations in glucose uptake, metabolism or oxidation. KATP inactivation by NN414 was prevented by the concurrent presence of tolbutamide, which maintains KATP closure. Single channel recordings indicate that NN414 alters KATP intrinsic gating inducing a stable closed or inactivated state. These data indicate that exposure of hypothalamic glucose sensing cells to chronic NN414 drives a sustained conformational change to KATP, probably by binding to SUR1, that results in loss of channel sensitivity to intrinsic metabolic factors such as MgADP and small molecule agonists.

  3. Correlation between the NPPB gene promoter c.-1298 G/T polymorphism site and pulse pressure in the Chinese Han population.

    PubMed

    Zeng, K; Wu, X D; Cai, H D; Gao, Y G; Li, G; Liu, Q C; Gao, F; Chen, J H; Lin, C Z

    2014-04-29

    The aim of this study was to investigate the correlation between the natriuretic peptide precursor B (NPPB) gene single nucleotide polymorphism (SNP) c.-1298 G/T and pulse pressure (PP) of the Chinese Han population and the association between genotype and clinical indicators of hypertension. Peripheral blood was collected from 180 unrelated patients with hypertension and 540 healthy volunteers (control group), and DNA was extracted to amplify the 5'-flanking region and 2 exons of the NPPB gene by polymerase chain reaction; the fragment was sequenced after purification. The clinical data of all subjects were recorded, the distribution of the NPPB gene c.-1298 G/T polymorphism was determined, and differences in clinical indicators between the two groups were evaluated. The mean arterial pressure PP, and creatinine levels were significantly higher in the hypertension group than in the control group (P<0.05), but no other clinical indicators differed between the groups. There were no significant differences in genotype frequency and distribution of the NPPB gene c.-1298 G/T polymorphism between the hypertension group and the control group (P>0.05); in the control group, the mean PP of individuals with the SNP c.-1298 GG genotype was greater than that of individuals with the GT+TT genotype (P<0.05). In conclusion, there was no significant correlation between the NPPB gene c.-1298 G/T polymorphism and the incidence of essential hypertension in the Han population; however, the PP of the SNP c.-1298 GG genotype was greater than that of the GT+TT genotype in the control group.

  4. Health-based reference intervals for ALAT, ASAT and GT in serum, measured according to the recommendations of the European Committee for Clinical Laboratory Standards (ECCLS).

    PubMed

    Leino, A; Impivaara, O; Irjala, K; Mäki, J; Peltola, O; Järvisalo, J

    1995-05-01

    The reference intervals for the activities of L-alanine aminotransferase (EC 2.6.1.2, ALAT), L-aspartate aminotransferase (EC 2.6.1.1, ASAT) and gamma-glutamyltransferase (EC 2.3.2.2, GT) in serum were determined according to the recommendations of the European Committee for Clinical Laboratory Standards (ECCLS). Serum specimens from 954 subjects were analysed for ALAT and ASAT and from 794 subjects for GT. The subjects, aged 27-67 years, were participants in general health surveys. The reference population was formed by excluding subjects with any disease, or on any medication, affecting the liver, and also those consuming excessive amounts of alcohol. The 95% inner reference intervals for ALAT and ASAT were 9-50 (n = 189) and 15-36 U l-1 (n = 192) in men and 8-38 (n = 270) and 13-33 U l-1 (n = 270) in women. For GT the reference interval was 11-58 in men (n = 165) and 8-42 U l-1 in women (n = 220). Serum GT levels correlated clearly with alcohol consumption. Serum ALAT and ASAT were only slightly associated with alcohol consumption at levels less than 280 g per week in men and 190 g per week in women. There were modest positive associations between the three enzyme levels and body mass index. None of the enzymes correlated significantly with age.

  5. Ameliorative effects of SLC22A2 gene polymorphism 808 G/T and cimetidine on cisplatin-induced nephrotoxicity in Chinese cancer patients.

    PubMed

    Zhang, Jing; Zhou, Wen

    2012-07-01

    To investigate the roles of SLC22A2 gene polymorphism 808 G/T and cimetidine on cisplatin-induced nephrotoxicity, a total of 123 Chinese cancer patients treated with cisplatin alone (n = 55) or in combination with cimetidine (n = 68) were genotyped. The changes of serum creatinine (SCr), blood urea nitrogen (BUN) and cystatin C levels were used as biomarkers for the evaluation of cisplatin-induced nephrotoxicity. The changes of BUN and SCr levels showed no significant difference between groups divided by genotypes and treatments (P > 0.05). However, patients with mutant genotype (GT/TT) or with cimetidine treatment had smaller increase of the cystatin C levels compared to those with wild genotype (GG) or without cimetidine treatment (P < 0.05). In the non-cimetidine-treated group, the changes of cystatin C level in patients with mutant genotype (GT/TT) was significantly smaller than those with wild genotype (GG) (P = 0.043). In the wild type group, the cystatin C level change of patients without cimetidine treatment was significantly larger than those with cimetidine treatment (P = 0.007). These results suggested that SLC22A2 gene polymorphism 808 G/T and cimetidine could attenuate cisplatin nephrotoxicity in Chinese cancer patients. But the renoprotection mechanism of cimetidine might be damaged by the mutation.

  6. Sensitive detection of the c-KIT c.1430G>T mutation by mutant-specific polymerase chain reaction in feline mast cell tumours.

    PubMed

    Takanosu, M; Sato, M; Kagawa, Y

    2014-06-01

    Here, we describe the establishment of mutant-specific polymerase chain reaction (PCR) for detection of a c-KIT c.1430G>T mutation in feline mast cell tumours. Several mutations in feline c-KIT have been identified, with the c.1430G>T mutation accounting for a significant portion of feline mast cell tumour mutations. The c.1430G>T mutation in c-KIT exon 9 was detected in 15.7% (11 of 70) of samples by mutant-specific PCR but in only 7.1% (5 of 70) by PCR-restriction fragment length polymorphism (RFLP) in the genomic DNA isolated from 70 formalin-fixed paraffin-embedded sections or cells collected by fine needle aspiration. Mutant-specific PCR showed remarkably higher detection rate than did PCR-RFLP. DNA sequence analysis did not always yield identical results to those of mutant-specific PCR, suggesting heterogeneity of tumour cells. Mutant-specific PCR is a valid and efficient screening tool for detection of the c-KIT c.1430G>T point mutation in feline mast cell tumours compared with PCR-RFLP and sequencing analysis.

  7. Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red-fleshed kiwifruit (Actinidia chinensis).

    PubMed

    Montefiori, Mirco; Espley, Richard V; Stevenson, David; Cooney, Janine; Datson, Paul M; Saiz, Anna; Atkinson, Ross G; Hellens, Roger P; Allan, Andrew C

    2011-01-01

    Much of the diversity of anthocyanins is due to the action of glycosyltransferases, which add sugar moieties to anthocyanidins. We identified two glycosyltransferases, F3GT1 and F3GGT1, from red-fleshed kiwifruit (Actinidia chinensis) that perform sequential glycosylation steps. Red-fleshed genotypes of kiwifruit accumulate anthocyanins mainly in the form of cyanidin 3-O-xylo-galactoside. Genes in the anthocyanin and flavonoid biosynthetic pathway were identified and shown to be expressed in fruit tissue. However, only the expression of the glycosyltransferase F3GT1 was correlated with anthocyanin accumulation in red tissues. Recombinant enzyme assays in vitro and in vivo RNA interference (RNAi) demonstrated the role of F3GT1 in the production of cyanidin 3-O-galactoside. F3GGT1 was shown to further glycosylate the sugar moiety of the anthocyanins. This second glycosylation can affect the solubility and stability of the pigments and modify their colour. We show that recombinant F3GGT1 can catalyse the addition of UDP-xylose to cyanidin 3-galactoside. While F3GGT1 is responsible for the end-product of the pathway, F3GT1 is likely to be the key enzyme regulating the accumulation of anthocyanin in red-fleshed kiwifruit varieties.

  8. Metabolic Thresholds and Validated Accelerometer Cutoff Points for the Actigraph GT1M in Young Children Based on Measurements of Locomotion and Play Activities

    ERIC Educational Resources Information Center

    Jimmy, Gerda; Dossegger, Alain; Seiler, Roland; Mader, Urs

    2012-01-01

    The purpose of the current study was to determine metabolic thresholds and subsequent activity intensity cutoff points for the ActiGraph GT1M with various epochs spanning from 5 to 60 sec in young children. Twenty-two children, aged 4 to 9 years, performed 10 different activities including locomotion and play activities. Energy expenditure was…

  9. Role of Key Salt Bridges in Thermostability of G. thermodenitrificans EstGtA2: Distinctive Patterns within the New Bacterial Lipolytic Enzyme Family XV

    PubMed Central

    Charbonneau, David M.; Beauregard, Marc

    2013-01-01

    Bacterial lipolytic enzymes were originally classified into eight different families defined by Arpigny and Jaeger (families I-VIII). Recently, the discovery of new lipolytic enzymes allowed for extending the original classification to fourteen families (I-XIV). We previously reported that G. thermodenitrificans EstGtA2 (access no. AEN92268) belonged to a novel group of bacterial lipolytic enzymes. Here we propose a 15th family (family XV) and suggest criteria for the assignation of protein sequences to the N’ subfamily. Five selected salt bridges, hallmarks of the N’ subfamily (E3/R54, E12/R37, E66/R140, D124/K178 and D205/R220) were disrupted in EstGtA2 using a combinatorial alanine-scanning approach. A set of 14 (R/K→A) mutants was produced, including five single, three double, three triple and three quadruple mutants. Despite a high tolerance to non-conservative mutations for folding, all the alanine substitutions were destabilizing (decreasing Tm by 5 to 14°C). A particular combination of four substitutions exceeded this tolerance and prevents the correct folding of EstGtA2, leading to enzyme inactivation. Although other mutants remain active at low temperatures, the accumulation of more than two mutations had a dramatic impact on EstGtA2 activity at high temperatures suggesting an important role of these conserved salt bridge-forming residues in thermostability of lipolytic enzymes from the N’ subfamily. We also identified a particular interloop salt bridge in EstGtA2 (D194/H222), located at position i -2 and i -4 residues from the catalytic Asp and His respectively which is conserved in other related bacterial lipolytic enzymes (families IV and XIII) with high tolerance to mutations and charge reversal. We investigated the role of residue identity at position 222 in controlling stability-pH dependence in EstGtA2. The introduction of a His to Arg mutation led to increase thermostability under alkaline pH. Our results suggest primary targets for

  10. Evaluation of rs62527607 [GT] single nucleotide polymorphism located in BAALC gene in children with acute leukemia using mismatch PCR-RFLP.

    PubMed

    Nadimi, Motahareh; Rahgozar, Soheila; Moafi, Alireza; Tavassoli, Manoochehr; Mesrian Tanha, Hamzeh

    2016-01-01

    Acute leukemia is the most common cancer in children and involves several factors that contribute to the development of multidrug resistance and treatment failure. According to our recent studies, the BAALC gene is identified to have high mRNA expression levels in childhood acute lymphoblastic leukemia (ALL) and those with multidrug resistance. Several polymorphisms are associated with the expression of this gene. To date, there has been no study on the rs62527607 [GT] single nucleotide polymorphism (SNP) of BAALC gene and its link with childhood acute lymphoblastic and myeloid leukemia (AML). The purpose of this study is to evaluate the prevalence of this polymorphism in pediatric acute leukemia, as well as its relationship with prognosis. DNA samples were extracted from bone marrow slides of 129 children with ALL and 16 children with AML. The rs62527607 [GT] SNP was evaluated using mismatch polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)-based analysis. The association between the SNP alleles and patient disease-free survival was then assessed. The prevalence of the T-allele of rs62527607 [GT] SNP in childhood T-ALL and pre-B-ALL was 28.3% and 11.2%, respectively. In the pre-B-ALL patients, 3 year disease free survival was associated with the GG genotype. Results showed a robust association between the rs62527607 SNP and the risk of relapse in ALL, but not AML, patients. T-ALL patients with the GT genotype had an 8.75 fold higher risk of relapse. The current study demonstrates a significant association between the genotype GT and the polymorphic allele G424T, and introduces this SNP as a negative prognostic factor in children with ALL.

  11. GT-repeat polymorphism in the heme oxygenase-1 gene promoter is associated with cardiovascular mortality risk in an arsenic-exposed population in northeastern Taiwan

    SciTech Connect

    Wu, Meei-Maan; Chiou, Hung-Yi; Chen, Chi-Ling; Wang, Yuan-Hung; Hsieh, Yi-Chen; Lien, Li-Ming; Lee, Te-Chang; Chen, Chien-Jen

    2010-11-01

    Inorganic arsenic has been associated with increased risk of atherosclerotic vascular disease and mortality in humans. A functional GT-repeat polymorphism in the heme oxygenase-1 (HO-1) gene promoter is inversely correlated with the development of coronary artery disease and restenosis after clinical angioplasty. The relationship of HO-1 genotype with arsenic-associated cardiovascular disease has not been studied. In this study, we evaluated the relationship between the HO-1 GT-repeat polymorphism and cardiovascular mortality in an arsenic-exposed population. A total of 504 study participants were followed up for a median of 10.7 years for occurrence of cardiovascular deaths (coronary heart disease, cerebrovascular disease, and peripheral arterial disease). Cardiovascular risk factors and DNA samples for determination of HO-1 GT repeats were obtained at recruitment. GT repeats variants were grouped into the S (< 27 repeats) or L allele ({>=} 27 repeats). Relative mortality risk was estimated using Cox regression analysis, adjusted for competing risk of cancer and other causes. For the L/L, L/S, and S/S genotype groups, the crude mortalities for cardiovascular disease were 8.42, 3.10, and 2.85 cases/1000 person-years, respectively. After adjusting for conventional cardiovascular risk factors and competing risk of cancer and other causes, carriers with class S allele (L/S or S/S genotypes) had a significantly reduced risk of cardiovascular mortality compared to non-carriers (L/L genotype) [OR, 0.38; 95% CI, 0.16-0.90]. In contrast, no significant association was observed between HO-1 genotype and cancer mortality or mortality from other causes. Shorter (GT)n repeats in the HO-1 gene promoter may confer protective effects against cardiovascular mortality related to arsenic exposure.

  12. Study on Off-Design Steady State Performances of Helium Gas Turbo-compressor for HTGR-GT

    SciTech Connect

    Qisen Ren; Xiaoyong Yang; Zhiyong Huang; Jie Wang

    2006-07-01

    The high temperature gas-cooled reactor (HTGR) coupled with direct gas turbine cycle is a promising concept in the future of nuclear power development. Both helium gas turbine and compressor are key components in the cycle. Under normal conditions, the mode of power adjustment is to control total helium mass in the primary loop using gas storage vessels. Meanwhile, thermal power of reactor core is regulated. This article analyzes off-design performances of helium gas turbine and compressors for high temperature gas-cooled reactor with gas turbine cycle (HTGR-GT) at steady state level of electric power adjustment. Moreover, performances of the cycle were simply discussed. Results show that the expansion ratio of turbine decreases as electric power reduces but the compression ratios of compressors increase, efficiencies of both turbine and compressors decrease to some extent. Thermal power does not vary consistently with electric power, the difference between these two powers increases as electric power reduces. As a result of much thermal energy dissipated in the temperature modulator set at core inlet, thermal efficiency of the cycle has a widely reduction under partial load conditions. (authors)

  13. The activity of interleukin-4 receptor alpha-chain promoter is regulated by a GT box element.

    PubMed

    Dorado, Beatriz; Martín-Saavedra, Francisco M; Jerez, María J; Ballester, Sara

    2006-04-01

    Interleukin-4 receptor (IL-4R) is the cell surface complex through which interleukin-4 (IL-4) signals exert its critical biological effects. The alpha-chain of IL-4R is responsible for the high affinity binding of IL-4. In this report, is characterized, the 5' untranslated flanking region of murine IL-4Ralpha gene in the Th2 clone D10.G4.1. We have analyzed a DNA fragment spanning from -995 to +84 relative to the transcription start point. Mutagenesis analysis shows that, neither the previously described Stat6 (-395) nor the NFAT (-266) and NFkappaB (+25) sequences localized here, are involved in the IL-4Ralpha promoter activity. Reporter assays demonstrate that maximum transcriptional activity is achieved by the -89 to +84 sequence and this activity is independent of a TATA-like box located at -25. We have identified a GT box located at -45 as the critical element for the IL-4Ralpha promoter activity. Experiments in SL2 cells, which lack endogenous Sp proteins, show that IL-4Ralpha minimal promoter is transactivated by proteins of Sp family.

  14. Cleaning efficiency of nickel-titanium GT and .04 rotary files when used in a torque-controlled rotary handpiece.

    PubMed

    Suffridge, Calvin B; Hartwell, Gary R; Walker, Thomas L

    2003-05-01

    This study determined if the cleaning efficiency of nickel-titanium rotary files in an endodontic electric handpiece using a no-torque control setting was superior to that obtained when using the torque-control feature. Fifty extracted human anterior teeth with straight canals were divided into two groups of 20 and two control groups of 5. Canals were instrumented with GT and .04 ProFile nickel-titanium files until a size 35 advanced to working length. Samples were sectioned and the apical 6 mm of the canal was photographed (x20) and projected onto a 3- x 4-foot grid with squares measuring 0.5 inches each. Total debris was the percentage of the number of squares containing debris versus the total number of squares. The teeth in the torque-controlled group showed an average of 24.99% debris versus 15.55% for the teeth in the no-torque group. The difference was not statistically significant; therefore, no difference can be said to exist between the two torque settings in terms of cleaning efficiency.

  15. Glutamate increases toxicity of inorganic lead in GT1-7 neurons: partial protection induced by flunarizine.

    PubMed

    Loikkanen, Jarkko; Naarala, Jonne; Vähäkangas, Kirsi H; Savolainen, Kai M

    2003-12-01

    Recent studies point to an interaction between the glutamatergic neurotransmitter system and inorganic lead (Pb) neurotoxicity. Pb (1-100 microM) evoked cytotoxicity over the period of 72 h in mouse hypothalamic GT1-7 neurons. Glutamate (0.1 or 1 mM) on its own did not have any effect on cell viability. However, 1 mM glutamate clearly increased Pb-induced cell death at 48 and 72 h. Although flunarizine (0.1-10 microM), an antagonist of L- and T-type voltage-sensitive calcium channels (VSCCs), partially protected from the cytotoxicity induced by co-exposure to Pb (10 or 100 micro M) and glutamate (1 mM), it had no protective effect on cytotoxicity induced by Pb alone. The flunarizine-induced protection was dependent on time and observed only at 48 h. Neither verapamil, an antagonist of L-type VSCCs, nor DIDS, an inhibitor of anion exchange, at non-toxic concentrations (0.1-10 microM) had any effect on cytotoxicity induced by Pb alone or together with glutamate at any studied time point. Co-exposure to Pb and glutamate also resulted in more prominent production of reactive oxygen species (ROS) than either of the compounds alone. Interestingly, we observed an increase in intracellular glutathione (GSH) levels in cells exposed to micromolar concentrations of Pb. Glutamate decreased the levels of intracellular GSH and also partially reduced the Pb-induced increase in GSH levels. These results suggest that the interaction of glutamate and Pb results in increased neuronal cell death via mechanisms that involve an increase in ROS production, a decrease in intracellular GSH defense against oxidative stress and probably T-type VSCCs.

  16. Hydrogen Fueled Hybrid Solid Oxide Fuel Cell-Gas Turbine (SOFC-GT) System for Long-Haul Rail Application

    NASA Astrophysics Data System (ADS)

    Chow, Justin Jeff

    Freight movement of goods is the artery for America's economic health. Long-haul rail is the premier mode of transport on a ton-mile basis. Concerns regarding greenhouse gas and criteria pollutant emissions, however, have motivated the creation of annually increasing locomotive emissions standards. Health issues from diesel particulate matter, especially near rail yards, have also been on the rise. These factors and the potential to raise conventional diesel-electric locomotive performance warrants the investigation of using future fuels in a more efficient system for locomotive application. This research evaluates the dynamic performance of a Solid Oxide Fuel Cell-Gas Turbine (SOFC-GT) Hybrid system operating on hydrogen fuel to power a locomotive over a rail path starting from the Port of Los Angeles and ending in the City of Barstow. Physical constraints, representative locomotive operation logic, and basic design are used from a previous feasibility study and simulations are performed in the MATLAB Simulink environment. In-house controls are adapted to and expanded upon. Results indicate high fuel-to-electricity efficiencies of at least 54% compared to a conventional diesel-electric locomotive efficiency of 35%. Incorporation of properly calibrated feedback and feed-forward controls enables substantial load following of difficult transients that result from train kinematics while maintaining turbomachinery operating requirements and suppressing thermal stresses in the fuel cell stack. The power split between the SOFC and gas turbine is deduced to be a deterministic factor in the balance between capital and operational costs. Using hydrogen results in no emissions if renewable and offers a potential of 24.2% fuel energy savings for the rail industry.

  17. Association of RAD 51 135 G/C, 172 G/T and XRCC3 Thr241Met gene polymorphisms with increased risk of head and neck cancer.

    PubMed

    Kayani, Mahmood Akhtar; Khan, Sumeera; Baig, Ruqia Mehmood; Mahjabeen, Ishrat

    2014-01-01

    Homologous recombination repair (HRR) plays an important role in protection against carcinogenic factors. Genes regulating the HRR mechanisms may impair their functions and consequently result in increased cancer susceptibility. RAD 51 and XRCC3 are key regulators of the HRR pathway and genetic variability in these may contribute to the appearance and progression of various cancers including head and neck cancer (HNC). The aim of the present study was to compare the distribution of genotypes of RAD51 (135G/C, 172 G/T) and XRCC3 (Thr241Met) polymorphisms between HNC patients and controls. Each polymorphism was genotyped using the polymerase chain reaction-restriction fragment length polymerase (PCR-RFLP) technique in 200 pathologically confirmed HNC patients along with 150 blood samples from normal, disease free healthy individuals. We observed that homozygous variant CC genotype of RAD51 135G/C was associated with a 2.5 fold increased HNC risk (OR=2.5; 95%CI=0.69-9.53; p<0.02), while second polymorphism of RAD 51 172 G/T, heterozygous variant GT genotype was associated with a 1.68 fold (OR=1.68; 95%CI=1.08-2.61; p<0.02) elevation when compared with controls. In the case of the Thr241Met polymorphism of XRCC3, we observed a 16 fold (OR=16; 95% CI= 3.78-69.67; p<0.0002) increased HNC risk in patients compared to controls. These results further suggested that RAD51 (135G/C, 172 G/T) and XRCC3 (Thr241Met) polymorphisms may be effective biomarkers for genetic susceptibility to HNC. Larger studies are needed to confirm our findings and identify the underlying mechanisms.

  18. (GT)n Repeat Polymorphism in Heme Oxygenase-1 (HO-1) Correlates with Clinical Outcome after Myeloablative or Nonmyeloablative Allogeneic Hematopoietic Cell Transplantation

    PubMed Central

    Køllgaard, Tania; Kornblit, Brian; Petersen, Jesper; Klausen, Tobias Wirenfeldt; Mortensen, Bo Kok; Brændstrup, Peter; Sengeløv, Henrik; Høgdall, Estrid; Müller, Klaus; Vindeløv, Lars; Andersen, Mads Hald; thor Straten, Per

    2016-01-01

    Allogeneic hematopoietic cell transplantation (HCT) is a treatment for various hematologic diseases where efficacy of treatment is in part based on the graft versus tumour (GVT) activity of cells in the transplant. The cytoprotective enzyme heme oxygenase-1 (HO-1) is a rate-limiting enzyme in heme degradation and it has been shown to exert anti-inflammatory functions. In humans a (GT)n repeat polymorphism regulates the expression of HO-1. We conducted fragment length analyses of the (GT)n repeat in the promotor region of the gene for HO-1 in DNA from donors and recipients receiving allogeneic myeloablative- (MA) (n = 110) or nonmyeloablative- (NMA-) (n = 250) HCT. Subsequently, we compared the length of the (GT)n repeat with clinical outcome after HCT. We demonstrated that transplants from a HO-1high donor after MA-conditioning (n = 13) is associated with higher relapse incidence at 3 years (p = 0.01, n = 110). In the NMA-conditioning setting transplantation of HO-1low donor cells into HO-1low recipients correlated significantly with decreased relapse related mortality (RRM) and longer progression free survival (PFS) (p = 0.03 and p = 0.008, respectively). Overall, our findings suggest that HO-1 may play a role for the induction of GVT effect after allogeneic HCT. PMID:27997582

  19. Microenvironmental pH-modified solid dispersions to enhance the dissolution and bioavailability of poorly water-soluble weakly basic GT0918, a developing anti-prostate cancer drug: preparation, characterization and evaluation in vivo.

    PubMed

    Yang, Meiyan; He, Shaolong; Fan, Yunzhou; Wang, Yuli; Ge, Zhenzhong; Shan, Li; Gong, Wei; Huang, Xiaoli; Tong, Youzhi; Gao, Chunsheng

    2014-11-20

    The aim of the present work was to design a pH-modified solid dispersion (pH(M)-SD) that can improve the dissolution and bioavailability of poorly water-soluble weakly basic GT0918, a developing anti-prostate cancer drug. To select the appropriate acidifiers, a solubility test was carried out first. Solid dispersions (SDs) containing GT0918 and polyvinylpyrrolidone (PVP) were prepared using a solvent evaporation method and were characterized using dissolution studies in different media. The solid states of the SDs were investigated using scanning electron microscopy (SEM), powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC) and Fourier transformed infrared spectroscopy (FTIR). The in vivo pharmacokinetics of the pH(M)-SDs tablets were also studied in beagle dogs compared to the conventional tablets. The optimized pH(M)-SD (GT0918/PVP/citric acid, 1:2:2 weight ratio) exhibited a significant improvement in the dissolution behavior compared to both the physical mixture and the binary SDs. Solid-state characterization revealed that the amorphous formation of GT0918 in the SDs and the strong H-bonding were only found in the pH(M)-SDs containing citric acid. Furthermore, the GT0918-loaded pH(M)-SD tablets showed a higher AUC and a lower tmax compared to the conventional tablets. Accordingly, the pH(M)-SD might be an efficient route for enhancing the dissolution and bioavailability of poorly water-soluble GT0918.

  20. Mutual interaction of kisspeptin, estrogen and bone morphogenetic protein-4 activity in GnRH regulation by GT1-7 cells.

    PubMed

    Terasaka, Tomohiro; Otsuka, Fumio; Tsukamoto, Naoko; Nakamura, Eri; Inagaki, Kenichi; Toma, Kishio; Ogura-Ochi, Kanako; Glidewell-Kenney, Christine; Lawson, Mark A; Makino, Hirofumi

    2013-12-05

    Reproduction is integrated by interaction of neural and hormonal signals converging on hypothalamic neurons for controlling gonadotropin-releasing hormone (GnRH). Kisspeptin, the peptide product of the kiss1 gene and the endogenous agonist for the GRP54 receptor, plays a key role in the regulation of GnRH secretion. In the present study, we investigated the interaction between kisspeptin, estrogen and BMPs in the regulation of GnRH production by using mouse hypothalamic GT1-7 cells. Treatment with kisspeptin increased GnRH mRNA expression and GnRH protein production in a concentration-dependent manner. The expression levels of kiss1 and GPR54 were not changed by kisspeptin stimulation. Kisspeptin induction of GnRH was suppressed by co-treatment with BMPs, with BMP-4 action being the most potent for suppressing the kisspeptin effect. The expression of kisspeptin receptor, GPR54, was suppressed by BMPs, and this effect was reversed in the presence of kisspeptin. It was also revealed that BMP-induced Smad1/5/8 phosphorylation and Id-1 expression were suppressed and inhibitory Smad6/7 was induced by kisspeptin. In addition, estrogen induced GPR54 expression, while kisspeptin increased the expression levels of ERα and ERβ, suggesting that the actions of estrogen and kisspeptin are mutually enhanced in GT1-7 cells. Moreover, kisspeptin stimulated MAPKs and AKT signaling, and ERK signaling was functionally involved in the kisspeptin-induced GnRH expression. BMP-4 was found to suppress kisspeptin-induced GnRH expression by reducing ERK signaling activity. Collectively, the results indicate that the axis of kisspeptin-induced GnRH production is bi-directionally controlled, being augmented by an interaction between ERα/β and GPR54 signaling and suppressed by BMP-4 action in GT1-7 neuron cells.

  1. Sensitivity of an Elekta iView GT a-Si EPID model to delivery errors for pre-treatment verification of IMRT fields.

    PubMed

    Herwiningsih, Sri; Hanlon, Peta; Fielding, Andrew

    2014-12-01

    A Monte Carlo model of an Elekta iViewGT amorphous silicon electronic portal imaging device (a-Si EPID) has been validated for pre-treatment verification of clinical IMRT treatment plans. The simulations involved the use of the BEAMnrc and DOSXYZnrc Monte Carlo codes to predict the response of the iViewGT a-Si EPID model. The predicted EPID images were compared to the measured images obtained from the experiment. The measured EPID images were obtained by delivering a photon beam from an Elekta Synergy linac to the Elekta iViewGT a-Si EPID. The a-Si EPID was used with no additional build-up material. Frame averaged EPID images were acquired and processed using in-house software. The agreement between the predicted and measured images was analyzed using the gamma analysis technique with acceptance criteria of 3 %/3 mm. The results show that the predicted EPID images for four clinical IMRT treatment plans have a good agreement with the measured EPID signal. Three prostate IMRT plans were found to have an average gamma pass rate of more than 95.0 % and a spinal IMRT plan has the average gamma pass rate of 94.3 %. During the period of performing this work a routine MLC calibration was performed and one of the IMRT treatments re-measured with the EPID. A change in the gamma pass rate for one field was observed. This was the motivation for a series of experiments to investigate the sensitivity of the method by introducing delivery errors, MLC position and dosimetric overshoot, into the simulated EPID images. The method was found to be sensitive to 1 mm leaf position errors and 10 % overshoot errors.

  2. Serum ASAT, ALAT, ALP, LD, GT, and CK determined in the Cobas-Bio centrifugal analyser by the methods of the Scandinavian Committee on Enzymes.

    PubMed

    Izquierdo, J M; Sotorrío, P; Alvarez-Uría, J; Estrada, J M; Quirós, A

    1982-04-01

    The recommended methods of the Scandinavian Committee on Enzymes [4, 5, 6, 7, 8] have been applied to the Cobas-Bio centrifugal analyser. Reagents and serum volumes were scaled down and final molarities were kept equal. Serum volumes in microliters were as follows: ASAT 30, ALAT 30, ALP 3, LD 5, GT 20, and CK 10. Including the dead space of the sample cup, the volume needed to perform all six tests was 113 microliter. Within-run and between-run precision (CV%) were as follows: ASAT 1.32 and 1.95, ALAT 1.68 and 2.93, ALP 1.56 and 3.10, LD 1.63 and 4.44, GT 0.81 and 2.23, and CK 1.02 and 1.94. Mean deviations (%) from target values of two commercial sera were as follows: ASAT -0.3 and -0.4, ALAT -0.4 and -2.2, ALP -1.8 and -7.3, LD 0.4 and -6.2, GT 13.9 and -10.7, and CK -4.9 and -1.3. Results of all the methods correlated well with those obtained with their respective manual methods. Analytical time for 28 samples of each analyte was 10 min, apart from CK which was 14 min. Reagent cost per sample was 0.6, 0.9, 0.1, 0.3, 0.9, and 26 US cents respectively. All reagents were prepared in the laboratory, except those for CK which were bought from J.T. Baker (Phillipsburgh, NJ, USA). In conclusion, the methods keep the features of the manual methods but they are more precise and practicable, much faster and cheaper, and use minimal amounts of sera more convenient for paediatric work.

  3. Short (GT)n microsatellite repeats in the heme oxygenase-1 gene promoter are associated with antioxidant and anti-inflammatory status in Mexican pediatric patients with sepsis.

    PubMed

    Vázquez-Armenta, Gabriela; González-Leal, Natalia; J Vázquez-de la Torre, Mayra; Muñoz-Valle, José Francisco; Ramos-Márquez, Martha E; Hernández-Cañaveral, Iván; Plascencia-Hernández, Arturo; Siller-López, Fernando

    2013-01-01

    An adequate immune and antioxidant response is a key to the resolution of sepsis. Heme oxygenase-1 (HMOX1) is a stress protein with a polymorphic (GT)n repeat in its gene promoter that regulates its expression in response to oxidative injury, such as that present in sepsis. HMOX1 is the rate-limiting enzyme of heme degradation, and the heme breakdown products, CO, Fe, and bilirubin, are considered to be biologically active metabolites with direct or indirect antioxidant and anti-inflammatory properties. In this study, we investigated the inflammatory and antioxidant response and the relationship with the HMOX1 levels and HMOX1 polymorphism in Mexican septic pediatric patients. In a case-control pilot study, we enrolled 64 septic patients and 72 hospitalized control patients without a diagnosis of sepsis. DNA extracted from buffy coat was genotyped for HMOX1 (GT)n polymorphism by PCR and markers of antioxidant and inflammatory status were quantified in plasma by analysis of the oxygen radical absorbance capacity (ORAC), protein carbonyl (PC), interleukin (IL) 6, IL10, and HMOX1 levels. In septic children, oxidative and inflammatory markers were elevated, and HMOX1 levels were positively correlated with IL10 levels. Genotypic and allelic distribution of HMOX1 polymorphism showed no difference between groups. HMOX1 short-allele septic carriers (< 25 GT repeats) presented favorable ORAC, PC and IL10 levels. This study confirms that an active response against pediatric sepsis involves the expression of HMOX1 and IL10, suggesting that the high antioxidant status associated with HMOX1 short-allele septic carriers might provide a beneficial environment for sepsis resolution.

  4. A novel NF1 frame-shift mutation (c.702_703delGT) in a Chinese family with neurofibromatosis type 1.

    PubMed

    Cai, S P; Fan, N; Chen, J; Xia, Z L; Wang, Y; Zhou, X M; Yin, Y; Wen, T L; Xia, Q J; Liu, X Y; Wang, H Y

    2014-07-24

    This study aimed to characterize the clinical features of a Chinese pedigree with neurofibromatosis type 1 (NF1) and to identify mutations in the NF1 gene. In this three-generation family containing 8 members, 5 had been diagnosed with NF1 and the others were asymptomatic. All members of the family underwent complete medical examinations. Molecular genetic analyses were performed on all subjects included in the study. All exons of NF1 were amplified by polymerase chain reaction, sequenced, and compared with a reference database. Possible changes in function of the protein induced by amino acid variants were predicted by bioinformatic analysis. In this family, the 5 patients presented different clinical phenotypes, but all manifested typical café-au-lait macules. One novel frame-shift mutation, c.702_703delGT, in exon 7 of NF1 was identified in all affected family members, but not in the unaffected family members or in 102 normal controls. This mutation generates a premature stop codon at amino acid position 720. Additionally, a synonymous mutation c.702 G>A was found in 3 family members, including 2 affected and 1 normal individuals. In conclusion, our study suggests that a novel c.702_703delGT frame-shift mutation in NF1 is likely to be responsible for the pathogenesis of NF1 in this family. To the best of our knowledge, it is the first time that a c.702_703delGT mutation has been identified in a family with neurofibromatosis type 1.

  5. Comparison of computer codes (CE-THERM, FRAP-T5, GT3-FLECHT, and TRUMP-FLECHT) with data from the NRU-LOCA thermal hydraulic tests

    SciTech Connect

    Mohr, C.L.; Rausch, W.N.; Hesson, G.M.

    1981-07-01

    The LOCA Simulation Program in the NRU reactor is the first set of experiments to provide data on the behavior of full-length, nuclear-heated PWR fuel bundles during the heatup, reflood, and quench phases of a loss-of-coolant accident (LOCA). This paper compares the temperature time histories of 4 experimental test cases with 4 computer codes: CE-THERM, FRAP-T5, GT3-FLECHT, and TRUMP-FLECHT. The preliminary comparisons between prediction and experiment show that the state-of-the art fuel codes have large uncertainties and are not necessarily conservative in predicting peak temperatures, turn around times, and bundle quench times.

  6. Patterns of p53 G-->T transversions in lung cancers reflect the primary mutagenic signature of DNA-damage by tobacco smoke.

    PubMed

    Hainaut, P; Pfeifer, G P

    2001-03-01

    It is unquestionable that the major cause of lung cancer is cigarette smoking. p53 mutations are common in lung cancers from smokers but less common in non-smokers. A large fraction of the p53 mutations in lung cancers are G-->T transversions, a type of mutation that is infrequent in other tumors aside from hepatocellular carcinoma. Previous studies have indicated that there is a good correlation between G-->T transversion hotspots in lung cancers and sites of preferential formation of polycyclic aromatic hydrocarbon (PAH) adducts along the p53 gene. The origin of p53 mutations in lung cancer has been questioned by recent reports suggesting that there are no significant differences in p53 mutation spectra between smokers and non-smokers and between lung cancers and non-lung cancers [S.N. Rodin and A.S. Rodin (2000) Human lung cancer and p53: The interplay between mutagenesis and selection. P:roc. Natl Acad. Sci. USA, 97, 12244-12249]. We have re-assessed these issues by using the latest update of the p53 mutation database of the International Agency for Research on Cancer (14 051 entries) as well as recent data from the primary literature on non-smokers. We come to the conclusion that the p53 mutation spectra are different between smokers and non-smokers and that this difference is highly statistically significant (G-->T transversions are 30 versus 10%; P < 0.0001, chi2 test). A similar difference is seen between lung cancers and non-lung cancers. At a number of mutational hotspots common to all cancers, a large fraction of the mutations are G-->T transversions in lung cancers but are almost exclusively G-->A transitions in non-lung cancers. Our data reinforce the notion that p53 mutations in lung cancers can be attributed to direct DNA damage from cigarette smoke carcinogens rather than to selection of pre-existing endogenous mutations.

  7. Fibroblast growth factor-1 stimulation of quiescent NIH 3T3 cells increases G/T mismatch-binding protein expression.

    PubMed Central

    Donohue, P J; Feng, S L; Alberts, G F; Guo, Y; Peifley, K A; Hsu, D K; Winkles, J A

    1996-01-01

    Polypeptide growth factors promote cell-cycle progression in part by the transcriptional activation of a diverse group of specific genes. We have used an mRNA differential-display approach to identify several fibroblast growth factor (FGF)-1 (acidic FGF)-inducible genes in NIH 3T3 cells. Here we report that one of these genes, called FGF-regulated (FR)-3, is predicted to encode G/T mismatch-binding protein (GTBP), a component of the mammalian DNA mismatch correction system. The murine GTBP gene is transiently expressed after FGF-1 or calf serum treatment, with maximal mRNA levels detected at 12 and 18 h post-stimulation. FGF-1-stimulated NIH 3T3 cells also express an increased amount of GTBP as determined by immunoblot analysis. These results indicate that elevated levels of GTBP may be required during the DNA synthesis phase of the cell cycle for efficient G/T mismatch recognition and repair. PMID:8870641

  8. Ketoolivosyl-tetracenomycin C: a new ketosugar bearing tetracenomycin reveals new insight into the substrate flexibility of glycosyltransferase ElmGT

    PubMed Central

    Nybo, S. Eric; Shabaan, Khaled A.; Kharel, Madan K.; Salas, José A.; Méndez, Carmen; Sutardjo, Happy; Rohr, Jürgen

    2012-01-01

    A new tetracenomycin analogue, 8-demethyl-8-(4′-keto)-α-l-olivosyl-tetracenomycin C, was generated through combinatorial biosynthesis. Streptomyces lividans TK 24 (cos16F4) was used as a host for expression of a “sugar plasmid” (pKOL) directing the biosynthesis of NDP-4-keto-l-olivose. This strain harbors all of the genes necessary for production of 8-demethylt-etracenomycin C and the sugar flexible glycosyltransferase ElmGT. To the best of our knowledge, this report represents the first characterization of a tetracenomycin derivative decorated with a ketosugar moiety. Also, as far as we know, 4-keto-l-olivose has only been described as an intermediate of oleandomycin biosynthesis, but has not been described before as an appendage for a polyketide compound. Furthermore, this report gives further insight into the substrate flexibility of ElmGT to include an NDP-ketosugar, which is unusual and is rarely observed among glycosyltransferases from antibiotic biosynthetic pathways. PMID:22361136

  9. Event-specific plasmid standards and real-time PCR methods for transgenic Bt11, Bt176, and GA21 maize and transgenic GT73 canola.

    PubMed

    Taverniers, Isabel; Windels, Pieter; Vaïtilingom, Marc; Milcamps, Anne; Van Bockstaele, Erik; Van den Eede, Guy; De Loose, Marc

    2005-04-20

    Since the 18th of April 2004, two new regulations, EC/1829/2003 on genetically modified food and feed products and EC/1830/2003 on traceability and labeling of GMOs, are in force in the EU. This new, comprehensive regulatory framework emphasizes the need of an adequate tracing system. Unique identifiers, such as the transgene genome junction region or a specific rearrangement within the transgene DNA, should form the basis of such a tracing system. In this study, we describe the development of event-specific tracing systems for transgenic maize lines Bt11, Bt176, and GA21 and for canola event GT73. Molecular characterization of the transgene loci enabled us to clone an event-specific sequence into a plasmid vector, to be used as a marker, and to develop line-specific primers. Primer specificity was tested through qualitative PCRs and dissociation curve analysis in SYBR Green I real-time PCRs. The primers were then combined with event-specific TaqMan probes in quantitative real-time PCRs. Calibration curves were set up both with genomic DNA samples and the newly synthesized plasmid DNA markers. It is shown that cloned plasmid GMO target sequences are perfectly suitable as unique identifiers and quantitative calibrators. Together with an event-specific primer pair and a highly specific TaqMan probe, the plasmid markers form crucial components of a unique and straighforward tracing system for Bt11, Bt176, and GA21 maize and GT73 canola events.

  10. Role of G-->T transversions in the mutagenicity of alkylperoxyl radicals: induction of alkali-labile sites in bacteriophage M13mp19.

    PubMed

    Harkin, L A; Butler, L M; Burcham, P C

    1997-05-01

    The mutagenicity of peroxyl radicals, ubiquitous products of lipid peroxidation, was assessed using an in vitro M13 forward mutational assay. Single-stranded M13mp19 plasmids were incubated with a range of concentrations of the azo initiator 2,2'-azobis(2-amidinopropane) hydrochloride, and then transfected into competent, SOS-induced Escherichia coli JM105 cells. Incubation with peroxyl radicals produced a concentration-dependent decrease in phage survival, with a 500 microM concentration of the azo initiator reducing the transfection efficiency by more than 90% while inducing a corresponding 6-fold increase in lacZ alpha mutation frequencies. Peroxyl radical-induced mutagenesis was completely prevented by the peroxyl radical scavenger Trolox. Automated DNA sequence analysis of the lacZ alpha gene of 100 peroxyl radical-induced mutants revealed that the most frequent sequence changes were base pair substitutions (92/95), with G-->T transversions predominating (73/92). Alkaline treatment prior to transfection diminished the mutagenicity of damaged plasmids to a level resembling that of unmodified DNA. While abasic sites might account for the sensitivity to alkaline cleavage, the possibility that unidentified nonabasic alkaline-labile lesions also contribute to peroxyl radical mutagenesis cannot be excluded. Collectively, these findings raise the possibility that DNA damage caused by a major class of endogenous radicals contributes to one of the most common spontaneous mutational events, the G-->T transversion.

  11. [Construction of genomic library of L. interrogans serovar lai using lambda gt11 as the vector and a study of recombiant plasmid pDL121].

    PubMed

    Liu, H; Dai, B; Jing, B; Wu, W; Li, S; Fang, Z; Zhao, H; Ye, D; Yan, R; Liu, J; Song, S; Yang, Y; Zhang, Y; Liu, F; Tu, Y; Yang, H; Huang, Z; Liang, L; Hu, L; Zhao, M

    1997-03-01

    A genomic library of L. interrogans serovar lai strain 017 has been constructed using lambda gt11 as the vector. DNA was partially digested by two blunt-end restriction enzymes, then methylated with EcoR I methylase; after EcoR I linker was added to the DNA, the linker-ended DNA was ligated to the dephosphorylated EcoR I digested lambda gt11 arms. The recombined DNA was packaged in vitro, and used to transduct E. coli Y1090 for amplification. There were 2.1 x 10(6) recombinant bacteriophages as recognized by their ability to form white plaques plated on Lac host in the presence of both IPTG and X-Ga1. A positive clone, designated lambda DL12, was screened with a rabbit anti-serum against L. interrogans serovar lai from the genomic library. The DNA from lambda DL12 was subcloned into plasmid pUC18. A recombinant (designated as pDL121) was obtained. SDS-PAGE analysis indicated that a 23 kd was expressed in E. coli JM 103 harboring pDL121. Western blotting analysis showed that a specific protein band molecular weight of 23 kd could be recognized by the rabbit antiserum against L. interrogans serovar lai strain 017.

  12. Toll-like receptor 7 Gln11Leu, c.4-151A/G, and +1817G/T polymorphisms in Crimean Congo hemorrhagic fever.

    PubMed

    Arslan, Serdal; Engin, Aynur; Özbilüm, Nil; Bakır, Mehmet

    2015-07-01

    Crimean-Congo hemorrhagic fever (CCHF) is a viral zoonosis. Toll-like receptors (TLRs) initiate signaling cascades leading to the activation of the innate immune system following CCHF infection. In this study, TLR7 (Gln11Leu, c.4-151A/G, and +1817G/T) polymorphisms were investigated in CCHF patients using polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP). The study population comprised 149 CCHF patients and 171 controls. For the TLR7 Gln11Leu polymorphism, there was no significant difference between the case and control groups in allele (P = 0.144) and genotype frequencies (P = 0.219). In the TLR7 IVS1 +1817G/T polymorphism, a statistically significant difference was found in allele frequencies (P = 0.026), but there was no significant difference in the TLR7 c.4-151A/G polymorphism (P = 0.310). There was a statistically significant difference in the distribution of the TLR7 c.4-151GG genotypes frequencies between patients and controls (P = 0.042; OR = 2.23). Furthermore, there were statistically significant associations between the TLR7 c.4-151A/G polymorphism and both severe disease and patient mortality (P < 0.001 and P = 0.047, respectively). The TLR7 IVS1 +1817TT genotype was also significantly associated with the case group but not the control group (P = 0.045). A strong positive linkage among TLR 7 variants was found using haplotype analysis. The incidence of two haplotypes, AGG and AGT, was determined to exhibit significant differences between the case and control groups (P < 0.001 and P < 0.001, respectively). These findings suggest that the TLR7 IVS1 +1817G/T and TLR7 c.4-151A/G polymorphisms may be important in the susceptibility or clinical course of CCHF disease.

  13. 5-Methylcytosine DNA glycosylase activity is also present in the human MBD4 (G/T mismatch glycosylase) and in a related avian sequence.

    PubMed

    Zhu, B; Zheng, Y; Angliker, H; Schwarz, S; Thiry, S; Siegmann, M; Jost, J P

    2000-11-01

    A 1468 bp cDNA coding for the chicken homolog of the human MBD4 G/T mismatch DNA glycosylase was isolated and sequenced. The derived amino acid sequence (416 amino acids) shows 46% identity with the human MBD4 and the conserved catalytic region at the C-terminal end (170 amino acids) has 90% identity. The non-conserved region of the avian protein has no consensus sequence for the methylated DNA binding domain. The recombinant proteins from human and chicken have G/T mismatch as well as 5-methylcytosine (5-MeC) DNA glycosylase activities. When tested by gel shift assays, human recombinant protein with or without the methylated DNA binding domain binds equally well to symmetrically, hemimethylated DNA and non-methylated DNA. However, the enzyme has only 5-MeC DNA glycosylase activity with the hemimethylated DNA. Footprinting of human MBD4 and of an N-terminal deletion mutant with partially depurinated and depyrimidinated substrate reveal a selective binding of the proteins to the modified substrate around the CpG. As for 5-MeC DNA glycosylase purified from chicken embryos, MBD4 does not use oligonucleotides containing mCpA, mCpT or mCpC as substrates. An mCpG within an A+T-rich oligonucleotide is a much better substrate than an A+T-poor sequence. The K:(m) of human MBD4 for hemimethylated DNA is approximately 10(-7) M with a V:(max) of approximately 10(-11) mol/h/microgram protein. Deletion mutations show that G/T mismatch and 5-MeC DNA glycosylase are located in the C-terminal conserved region. In sharp contrast to the 5-MeC DNA glycosylase isolated from the chicken embryo DNA demethylation complex, the two enzymatic activities of MBD4 are strongly inhibited by RNA. In situ hybridization with antisense RNA indicate that MBD4 is only located in dividing cells of differentiating embryonic tissues.

  14. The 894G>T endothelial nitric oxide synthase genetic polymorphism affects hemodynamic responses to mental stress performed before and after exercise.

    PubMed

    Rocha, Natália Galito; Neves, Fabricia Junqueira; Silva, Bruno Moreira; Sales, Allan Robson Kluser; Nóbrega, Antonio Claudio

    2012-03-01

    Nitric oxide is the primary mediator of vasodilation during mental stress. Since genetic polymorphisms in the nitric oxide synthase (eNOS) gene seem to impair the production of NO, this study aimed to evaluate the effect of an exercise bout on hemodynamic responses to mental stress in subjects with the 894G>T polymorphism of eNOS. Subjects without (wild-type group; n = 16) or with (polymorphic-type group; n = 19) the 894G>T polymorphism underwent a mental stress challenge before and after a maximal cardiopulmonary exercise test. Blood pressure was measured by auscultation and forearm blood flow by venous occlusion plethysmography. The groups were similar regarding anthropometric, metabolic, resting blood pressure and exercise variables. Before exercise, systolic blood pressure response during mental stress was higher in the polymorphic-type group (∆wild-type: 8.0 ± 2.0% vs. ∆polymorphic-type: 12.5 ± 1.8%, P = 0.01), while the increase in forearm vascular conductance was similar between the groups (∆wild-type 90.8 ± 26.4% vs. ∆polymorphic-type: 86.3 ± 24.1%, P = 0.44). After exercise, the systolic blood pressure at baseline and during mental stress was lower than before exercise in the whole group (P < 0.05), but the pressure response during mental stress was still higher in the polymorphic-type group (∆wild-type: 5.8 ± 1.5% vs. ∆polymorphic-type: 10.2 ± 1.4%, P = 0.01). The increase in forearm vascular conductance was inhibited only in the polymorphic-type group (∆before exercise 86.3 ± 24.1% vs. ∆after exercise: 41.5 ± 12.6%, P = 0.04). In conclusion, these results suggest the 894G>T eNOS polymorphism is associated with altered hemodynamic responses to mental stress both before and after a single bout of dynamic exercise with potential clinical implications.

  15. Association of the CYP2B6 c.516G>T polymorphism with high blood propofol concentrations in women from northern Greece.

    PubMed

    Mastrogianni, Orthodoxia; Gbandi, Emma; Orphanidis, Amvrosios; Raikos, Nikolaos; Goutziomitrou, Evangelia; Kolibianakis, Efstratios M; Tarlatzis, Basil C; Goulas, Antonis

    2014-01-01

    Cytochrome P450 2B6 (CYP2B6) is responsible for the initial biotransformation of profol, an extensively metabolized intravenous anesthetic. In this study we examined the effect of the apparently functional CYP2B6 c.516G>T polymorphism on the distribution of propofol concentrations, quantified by GC/MS analysis following a single bolus dose, in the blood of 44 Greek women undergoing oocyte retrieval. Univariate analysis using age, height, weight and smoking status as covariates, as well as the Mann-Whitney non-parametric test, revealed a strong trend of association of the T allele with high propofol concentrations determined in whole blood, shortly after a single bolus dose. Propofol concentrations which were higher than one standard deviation of the mean were almost invariably associated with carriage of the T allele.

  16. Phenotypic expression and origin of the rare beta-thalassemia splice site mutation HBB:c.315 + 1G>T.

    PubMed

    Broquere, Cédrick; Brudey, Karine; Harteveld, Cornelis L; Saint-Martin, Christian; Elion, Jacques; Giordano, Piero C; Romana, Marc

    2010-06-01

    We present the hematological characteristics of five patients from Surinam and the bordering French Guyana, who are carriers of the rare beta-thalassemia (beta-thal) mutation HBB:c.315+1G>T. Analysis of the phenotype/genotype relationship shows that this allele is a beta(0)-thal variant and illustrates the modulating effect of the alpha-globin gene status on the beta-thal phenotype. The ethnic origin of the five probands, belonging to the so-called Bush Negroes Maroons of Surinam and French Guyana, strongly suggests that this beta-thal mutation has a West African origin and spread in this ethnic group because of a founder effect and/or genetic drift.

  17. Biasing Simulations of DNA Base Pair Parameters with Application to Propellor Twisting in AT/AT, AA/TT, and AC/GT Steps and Their Uracil Analogs.

    PubMed

    Peguero-Tejada, Alfredo; van der Vaart, Arjan

    2017-01-23

    An accurate and efficient implementation of the six DNA base pair parameters as order parameters for enhanced sampling simulations is presented. The parameter definitions are defined by vector algebra operations on a reduced atomic set of the base pair, and correlate very well with standard definitions. Application of the model is illustrated by umbrella sampling simulations of propeller twisting within AT/AT, AA/TT, and AC/GT steps and their uracil analogs. Strong correlations are found between propeller twisting and a number of conformational parameters, including buckle, opening, BI/BII backbone configuration, and sugar puckering. The thymine methyl group is observed to notably alter the local conformational free energy landscape, with effects within and directly upstream of the thymine containing base pair.

  18. Neurokinin B Causes Acute GnRH Secretion and Repression of GnRH Transcription in GT1–7 GnRH Neurons

    PubMed Central

    Glidewell-Kenney, Christine A.; Shao, Paul P.; Iyer, Anita K.; Grove, Anna M. H.; Meadows, Jason D.

    2013-01-01

    Genetic studies in human patients with idiopathic hypogonadotropic hypogonadism (IHH) identified mutations in the genes that encode neurokinin B (NKB) and the neurokinin 3 receptor (NK3R). However, determining the mechanism whereby NKB regulates gonadotropin secretion has been difficult because of conflicting results from in vivo studies investigating the luteinizing hormone (LH) response to senktide, a NK3R agonist. NK3R is expressed in a subset of GnRH neurons and in kisspeptin neurons that are known to regulate GnRH secretion. Thus, one potential source of inconsistency is that NKB could produce opposing direct and indirect effects on GnRH secretion. Here, we employ the GT1-7 cell model to elucidate the direct effects of NKB on GnRH neuron function. We find that GT1-7 cells express NK3R and respond to acute senktide treatment with c-Fos induction and increased GnRH secretion. In contrast, long-term senktide treatment decreased GnRH secretion. Next, we focus on the examination of the mechanism underlying the long-term decrease in secretion and determine that senktide treatment represses transcription of GnRH. We further show that this repression of GnRH transcription may involve enhanced c-Fos protein binding at novel activator protein-1 (AP-1) half-sites identified in enhancer 1 and the promoter, as well as chromatin remodeling at the promoter of the GnRH gene. These data indicate that NKB could directly regulate secretion from NK3R-expressing GnRH neurons. Furthermore, whether the response is inhibitory or stimulatory toward GnRH secretion could depend on the history or length of exposure to NKB because of a repressive effect on GnRH transcription. PMID:23393128

  19. Association of COMT val158met and DRD2 G>T genetic polymorphisms with individual differences in motor learning and performance in female young adults

    PubMed Central

    Boyden, Nate B.; Kwak, Youngbin; Humfleet, Jennifer; Burke, David T.; Müller, Martijn L. T. M.; Bohnen, Nico I.; Seidler, Rachael D.

    2013-01-01

    Individuals learn new skills at different rates. Given the involvement of corticostriatal pathways in some types of learning, variations in dopaminergic transmission may contribute to these individual differences. Genetic polymorphisms of the catechol-O-methyltransferase (COMT) enzyme and dopamine receptor D2 (DRD2) genes partially determine cortical and striatal dopamine availability, respectively. Individuals who are homozygous for the COMT methionine (met) allele show reduced cortical COMT enzymatic activity, resulting in increased dopamine levels in the prefrontal cortex as opposed to individuals who are carriers of the valine (val) allele. DRD2 G-allele homozygotes benefit from a higher striatal dopamine level compared with T-allele carriers. We hypothesized that individuals who are homozygous for COMT met and DRD2 G alleles would show higher rates of motor learning. Seventy-two young healthy females (20 ± 1.9 yr) performed a sensorimotor adaptation task and a motor sequence learning task. A nonparametric mixed model ANOVA revealed that the COMT val-val group demonstrated poorer performance in the sequence learning task compared with the met-met group and showed a learning deficit in the visuomotor adaptation task compared with both met-met and val-met groups. The DRD2 TT group showed poorer performance in the sequence learning task compared with the GT group, but there was no difference between DRD2 genotype groups in adaptation rate. Although these results did not entirely come out as one might predict based on the known contribution of corticostriatal pathways to motor sequence learning, they support the role of genetic polymorphisms of COMT val158met (rs4680) and DRD2 G>T (rs 1076560) in explaining individual differences in motor performance and motor learning, dependent on task type. PMID:24225542

  20. Effect of glutamate and extracellular calcium on uptake of inorganic lead (Pb2+) in immortalized mouse hypothalamic GT1-7 neuronal cells.

    PubMed

    Loikkanen, J; Naarala, J; Vähäkangas, K H; Savolainen, K M

    2006-01-25

    We have previously shown that although glutamate alone has no effects on viability of mouse hypothalamic GT1-7 cells, it clearly enhances Pb2+-induced cytotoxicity. It is likely that Pb2+ must enter cells to exert most of its toxic effects. Pb2+ is known to substitute for Ca2+ in many cellular processes. Therefore, we studied the uptake mechanisms of Pb2+ into GT1-7 neuronal cells with a special focus on the role of extracellular calcium (Ca2+), voltage-sensitive calcium channels (VSCCs) and glutamate. Basal uptake of Pb2+ (1 microM or 10 microM), i.e. without any external stimulus, clearly increased in nominally Ca2+-free buffer and was partially abolished by 13 mM Ca2+ when compared to uptake in the presence of a physiological concentration of extracellular Ca2+ (1.3 mM). Depolarization by 25 mM K+, or antagonists of VSCCs, verapamil (10 microM) or flunarizine (10 microM) had no clear effect on basal Pb2+ uptake. Glutamate (1 mM) increased Pb2+ uptake, but only when cells were treated with 1 microM Pb2+ in the presence of 1.3 mM Ca2+. Our data suggest that Pb2+ competes for the same cellular uptake pathways with Ca2+, although not via VSCCs. In addition, enhancement of Pb2+-induced neurotoxicity by glutamate may be due to increased neuronal uptake of Pb2+.

  1. The significance of c.690G>T polymorphism (rs34529039) and expression of the CEBPA gene in ovarian cancer outcome

    PubMed Central

    Kwiatkowska, Ewa; Podgorska, Agnieszka; Zolocinska, Aleksandra; Pienkowska-Grela, Barbara; Dansonka-Mieszkowska, Agnieszka; Balcerak, Anna; Lukasik, Martyna; Stachurska, Anna; Timorek, Agnieszka; Spiewankiewicz, Beata; El-Bahrawy, Mona; Kupryjanczyk, Jolanta

    2016-01-01

    The CEBPA gene is known to be mutated or abnormally expressed in several cancers. This is the first study assessing the clinical impact of CEBPA gene status and expression on the ovarian cancer outcome. The CEBPA gene sequence was analyzed in 118 ovarian cancer patients (44 platinum/cyclophosphamide (PC)-treated and 74 taxane/platinum (TP)-treated), both in tumors and blood samples, and in blood from 236 healthy women, using PCR-Sanger sequencing and Real-Time quantitative PCR (qPCR)-based genotyping methods, respectively. The CEBPA mRNA level was examined with Reverse Transcription quantitative PCR (RT-qPCR). The results were correlated to different clinicopathological parameters. Thirty of 118 (25.4%) tumors harbored the CEBPA synonymous c.690G>T polymorphism (rs34529039), that we showed to be related to up-regulation of CEBPA mRNA levels (p=0.0059). The presence of the polymorphism was significantly associated with poor prognosis (p=0.005) and poor response to the PC chemotherapy regimen (p=0.024). In accordance, elevated CEBPA mRNA levels negatively affected patient survival (p<0.001) and tumor response to the PC therapy (p=0.014). The rs34529039 SNP did not affect the risk of developing ovarian cancer. This is the first study providing evidence that the c.690G>T, p.(Thr230Thr) (rs34529039) polymorphism of the CEBPA gene, together with up-regulation of its mRNA expression, are negative factors worsening ovarian cancer outcome. Their adverse clinical effect depends on a therapeutic regimen used, which might make them potential prognostic and predictive biomarkers for response to DNA-damaging chemotherapy. PMID:27602952

  2. Induction of T(4) UDP-GT activity, serum thyroid stimulating hormone, and thyroid follicular cell proliferation in mice treated with microsomal enzyme inducers.

    PubMed

    Hood, Alan; Allen, Marcia L; Liu, YaPing; Liu, Jie; Klaassen, Curtis D

    2003-04-01

    The microsomal enzyme inducers phenobarbital (PB), pregnenolone-16 alpha-carbonitrile (PCN), 3-methylcholanthrene (3MC), and Aroclor 1254 (PCB) are known to induce thyroxine (T(4)) glucuronidation and reduce serum T(4) concentrations in rats. Also, microsomal enzyme inducers that increase serum TSH (i.e., PB and PCN) also increase thyroid follicular cell proliferation in rats. Little is known about the effects of these microsomal enzyme inducers on T(4) glucuronidation, serum thyroid hormone concentrations, serum TSH, and thyroid gland growth in mice. Therefore, we tested the hypothesis that microsomal enzyme inducers induce T(4) UDP-GT activity, resulting in reduced serum T(4) concentrations, as well as increased serum TSH and thyroid follicular cell proliferation in mice. B6C3F male mice were fed a control diet or a diet containing PB (600, 1200, 1800, or 2400 ppm), PCN (250, 500, 1000, or 2000 ppm), 3MC (62.5, 125, 250, or 500 ppm), or PCB (10, 30, 100, or 300 ppm) for 21 days. All four inducers increased liver weight and hepatic microsomal UDP-GT activity toward chloramphenicol, alpha-naphthol, and T(4). PB and PCB decreased serum total T(4), but PCN and 3MC did not. Serum thyroid stimulating hormone was markedly increased by PCN and 3MC treatments, and slightly increased by PB and PCB treatments. All four microsomal enzyme inducers dramatically increased thyroid follicular cell proliferation in mice. The findings suggest that PB, PCN, 3MC, and PCB disrupt thyroid hormone homeostasis in mice.

  3. STAT4 rs7574865 G/T polymorphism is associated with rheumatoid arthritis and disease activity, but not with anti-CCP antibody levels in a Mexican population.

    PubMed

    Durán-Avelar, Ma de Jesús; Vibanco-Pérez, Norberto; Hernández-Pacheco, Raquel Rocío; Castro-Zambrano, América Del Carmen; Ortiz-Martínez, Liliana; Zambrano-Zaragoza, José Francisco

    2016-12-01

    Rheumatoid arthritis (RA) is a systemic autoimmune disease in whose etiology genetic factors are known to play an important role. Among the genes associated with RA, STAT4 could be an important factor in conducting helper T cells toward the pro-inflammatory Th1 and Th17 lineages. The aim of this study is to determine the association of the STAT4 polymorphism rs7574865 with RA, disease activity, and anti-cyclic citrullinated peptide (CCP) antibody levels in a Mexican population. Genotyping was carried out using the Taqman® system from Applied Biosystems in 140 patients with RA and 150 healthy subjects. Disease activity was evaluated by a rheumatologist using the DAS28 and Spanish-HAQ-DI instruments. Anti-CCP levels were determined by ELISA. Associations of the genotypes of rs7574865 with DAS28, HAQ, and anti-CCP antibody levels with RA were determined. Findings showed that the GT and TT genotypes and the T allele from rs7574865 were all associated as risk factors for RA, independently of their anti-CCP status. An association with moderate-to-high disease activity (DAS28 ≥ 3.2) was also found. Additionally, patients with the GT or TT genotypes showed lower HAQ values than those who carried the GG genotype. No differences in anti-CCP antibody levels or DAS28 and genotypes were found. This work supports the association of the STAT4 rs7574865 polymorphism with RA and disease activity, but not with anti-CCP antibody levels in a Mexican population.

  4. Visfatin -948G/T and resistin -420C/G polymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases.

    PubMed

    Motawi, Tarek M K; Shaker, Olfat G; El-Sawalhi, Maha M; Abdel-Nasser, Zeinab M

    2014-05-01

    Diabetes mellitus is one of the main threats to human health in the 21st century. Visfatin/Nampt and resistin are novel adipokines that have been implicated in the pathogenesis of type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) complication. Several genetic studies have shown inconsistent results regarding association of visfatin/Nampt gene (NAMPT) and resistin gene (RETN) polymorphisms with T2DM and CVD complications. Here, we investigate whether NAMPT -948G/T and RETN -420C/G polymorphisms are associated with T2DM, its CVD complications, and serum adipokines levels in 90 Egyptian diabetic patients (44 without CVD and 46 with CVD) along with 60 healthy control subjects. Higher frequencies of NAMPT -948G/G and RETN -420G/G were observed among T2DM patients compared with controls. Furthermore, the frequencies of these genotypes were significantly higher in T2DM patients with CVD than those without CVD. Both NAMPT -948G/G and RETN -420G/G genotypes and G alleles were significantly associated with T2DM and CVD in Egyptian diabetic patients. Moreover, serum visfatin/Nampt and resistin levels were markedly elevated in T2DM patients, with the highest values observed in G/G genotypes among T2DM patients with CVD. In addition, positive correlations were observed between plasma adipokines levels and CVD risk factors. In conclusion, our data suggests that genetic variations in NAMPT -948G/T and RETN -420C/G may contribute to the disposition for T2DM and its CVD complications in Egyptian patients. However, further studies with greater sample size should be performed to verify these results.

  5. A game theory-reinforcement learning (GT-RL) method to develop optimal operation policies for multi-operator reservoir systems

    NASA Astrophysics Data System (ADS)

    Madani, Kaveh; Hooshyar, Milad

    2014-11-01

    Reservoir systems with multiple operators can benefit from coordination of operation policies. To maximize the total benefit of these systems the literature has normally used the social planner's approach. Based on this approach operation decisions are optimized using a multi-objective optimization model with a compound system's objective. While the utility of the system can be increased this way, fair allocation of benefits among the operators remains challenging for the social planner who has to assign controversial weights to the system's beneficiaries and their objectives. Cooperative game theory provides an alternative framework for fair and efficient allocation of the incremental benefits of cooperation. To determine the fair and efficient utility shares of the beneficiaries, cooperative game theory solution methods consider the gains of each party in the status quo (non-cooperation) as well as what can be gained through the grand coalition (social planner's solution or full cooperation) and partial coalitions. Nevertheless, estimation of the benefits of different coalitions can be challenging in complex multi-beneficiary systems. Reinforcement learning can be used to address this challenge and determine the gains of the beneficiaries for different levels of cooperation, i.e., non-cooperation, partial cooperation, and full cooperation, providing the essential input for allocation based on cooperative game theory. This paper develops a game theory-reinforcement learning (GT-RL) method for determining the optimal operation policies in multi-operator multi-reservoir systems with respect to fairness and efficiency criteria. As the first step to underline the utility of the GT-RL method in solving complex multi-agent multi-reservoir problems without a need for developing compound objectives and weight assignment, the proposed method is applied to a hypothetical three-agent three-reservoir system.

  6. A haplotype derived from the common variants at the -1997G/T and Sp1 binding site of the COL1A1 gene influences risk of postmenopausal osteoporosis in India.

    PubMed

    Singh, Monica; Singh, Puneetpal; Singh, Surinder; Juneja, Pawan Kumar; Kaur, Taranpal

    2013-02-01

    The aim of the present study was to investigate the association between Collagen 1 alpha 1 (COL1A1) polymorphism and osteoporosis in DEXA verified 349 (145 osteoporotic, 87 osteopenic and 117 normal) postmenopausal women of India, who were not taking hormone replacement therapy. Two single-nucleotide polymorphisms (SNPs), that is, -1997G/T (rs1107946) and +1245G/T (rs1800012, Sp1) of the COL1A1 gene, were analyzed. Minor allele frequencies of rs1107946 and rs1800012 were 0.15 and 0.20 in osteoporotic women, 0.18 and 0.18 in osteopenic and 0.20 and 0.17 in women having normal bone mass. An allele dose effect with BMD of lumbar spine has been exhibited by major allele G of rs1107946 (GG: 0.86 g/cm(2), GT: 0.91 g/cm(2) and TT: 0.93 g/cm(2)) and minor allele T of rs1800012 (GG: 0.91 g/cm(2), GT: 0.87 g/cm(2) and TT: 0.81 g/cm(2)). Disease association analysis revealed a haplotype GT that confers approximately threefold higher risk of osteoporosis in the carriers (OR 3.12, 95% CI 1.24-8.88, P = 0.008) after adjusting the confounding effect of age, BMI and years since menopause. These results suggest that GT haplotype of COL1A1 gene is associated with a higher risk of postmenopausal osteoporosis in Northwest Indian women.

  7. Novel mutations c.28G>T (p.Ala10Ser) and c.189G>T (p.Glu63Asp) in WDR62 associated with early onset acanthosis and hyperkeratosis in a patient with autosomal recessive microcephaly type 2

    PubMed Central

    Wu, Jing; Yang, Zhiyun; Deng, Weiping; Chen, Dongna; Deng, Jianlian; Su, Yan; Li, Yang; Wu, Chao; Wang, Ye; Zeng, Hao; Wang, Yiming; Li, Xunhua

    2016-01-01

    Microcephaly (MCPH) is a developmental disorder characterized by reduced brain size and intellectual disability. A proportion of microcephaly is caused by defects in a single gene. Microcephaly 2 (MCPH2) is one of the most frequent subtypes of MCPH.WD repeat-containing protein 62 gene (WDR62) is the most frequently mutated gene in MCPH2 patients. Phenotypes involving dermatological changes in MCPH2 have not been reported. We have identified and investigated a 5-year-old Chinese girl with markedly reduced brain size (86% of normal size), intellectual disability and psychomotor developmental delay. The patient also exhibited spattered blisters and reduced hair density on her head, anisochromasia with reticular hyperpigmentation and hypopigmentation on the trunk, which she has had since the age of 4 and had been found by her parents. Histological examination of a skin biopsy revealed acanthosis, hyperkeratosis and necrotic keratinocytes. Whole exome and Sanger sequencing identified two novel missense mutations, c.28G>T and c.189G>T, in the WDR62 gene. Both the mutations non-synonymously affect evolutionarily conserved amino acids and are predicted to be disease causing. We report the first case of MCPH2 that also presented with marked dermatological changes. Our findings expand the mutational and phenotypical spectra of MCPH2 and are valuable in the mutation-based pre- and post-natal screening and genetic diagnosis for MCPH2. PMID:27852057

  8. Genetic mapping and quantitative trait loci analysis for disease resistance using F2 and F5 generation-based genetic maps derived from 'Tifrunner' x'GT-C20' in peanut

    Technology Transfer Automated Retrieval System (TEKTRAN)

    One mapping population derived from Tifrunner × GT-C20 has shown great potential in developing a high dense genetic map and identification of QTLs for important disease resistance, Tomato spotted wilt virus (TSWV) and leaf spot (LS). Both F2 and F5 generation-based genetic maps were constructed prev...

  9. GnRH agonist reduces estrogen receptor dimerization in GT1-7 cells: evidence for cross-talk between membrane-initiated estrogen and GnRH signaling.

    PubMed

    Chason, Rebecca J; Kang, Jung-Hoon; Gerkowicz, Sabrina A; Dufau, Maria L; Catt, Kevin J; Segars, James H

    2015-03-15

    17β-estradiol (E2), a key participant on the initiation of the LH surge, exerts both positive and negative feedback on GnRH neurons. We sought to investigate potential interactions between estrogen receptors alpha (ERα) and beta (ERβ) and gonadotropin releasing hormone receptor (GnRH-R) in GT1-7 cells. Radioligand binding studies demonstrated a significant decrease in saturation E2 binding in cells treated with GnRH agonist. Conversely, there was a significant reduction in GnRH binding in GT1-7 cells treated with E2. In BRET(1) experiments, ERα-ERα dimerization was suppressed in GT1-7 cells treated with GnRH agonist (p < 0.05). There was no evidence of direct interaction between ERs and GnRH-R. This study provides the first evidence of reduced ERα homodimerization by GnRH agonist. Collectively, these findings demonstrate significant cross-talk between membrane-initiated GnRH and E2 signaling in GT1-7 cells.

  10. A new hemoglobin variant: Hb Meylan [β73(E17)Asp → Phe; HBB: c.220G>T; c.221A>T] with a double base mutation at the same codon.

    PubMed

    Renoux, Céline; Feray, Cécile; Joly, Philippe; Zanella-Cleon, Isabelle; Garcia, Caroline; Lacan, Philippe; Couprie, Nicole; Francina, Alain

    2015-01-01

    We report a new β-globin chain variant: Hb Meylan [β73(E17)Asp → Phe; HBB: c.220G>T; c.221A>T]. The new variant results from a double nucleotide mutation at the same codon. The possible molecular mechanisms are discussed.

  11. [Comparative analysis of rotatory (GT) and manual root canal preparation and association of both techniques in instrumentation of flattened root canals].

    PubMed

    Gonçalves, Silvana Beltrami; Brosco, Viviane Haiub; Bramante, Clovis Monteiro

    2003-03-01

    Root canal preparation has been considered one of the most important steps in root canal therapy, thus many techniques and instruments have been developed. The aim of this study was to evaluate the cleaning of the root canal through three different instrumentation techniques. Thirty mandibular incisors were selected and submitted to lingual access cavities. Afterwards, the canals were filled with India ink dye previously stored in carpules, which was inserted into the root canal by means of anesthetic syringe and anesthetic needles. After 48 hours, during which the dye was allowed to dry inside the root canal, the teeth were divided in three groups: G1- GT rotatory instrumentation; G2- manual instrumentation; G3- association of both. After instrumentation, the teeth were longitudinally sectioned. The cleaning process accomplished through the different instrumentation techniques was evidenced by dye removal at the cervical, middle and apical thirds of the root canal. The results of this study showed that were not statistically significant differences between these three instrumentation techniques for all three thirds of the root canal.

  12. A novel homozygous mutation IVS6+5G>T in CYP11B1 gene in a Vietnamese patient with 11β-hydroxylase deficiency.

    PubMed

    Nguyen, Thi Phuong Mai; Nguyen, Thu Hien; Ngo, Diem Ngoc; Vu, Chi Dung; Nguyen, Thi Kim Lien; Nong, Van Hai; Nguyen, Huy Hoang

    2015-07-10

    Congenital adrenal hyperplasia (CAH) is an autosomal recessive disease which is characterized by a deficiency of one of the enzymes involved in the synthesis of cortisol from cholesterol by the adrenal cortex. CAH cases arising from impaired 11β-hydroxylase are the second most common form. Mutations in the CYP11B1 gene are the cause of 11β-hydroxylase deficiency. This study was performed on a patient with congenital adrenal hyperplasia and with premature development such as enlarged penis, muscle development, high blood pressure, and bone age equivalent of 5 years old at 2 years of chronological age. Biochemical tests for steroids confirmed the diagnosis of CAH. We used PCR and sequencing to screen for mutations in CYP11B1 gene. Results showed that the patient has a novel homozygous mutation of guanine (G) to thymine (T) in intron 6 (IVS6+5G>T). The analysis of this mutation by MaxEntScan boundary software indicated that this mutant could affect the gene splicing during transcription.

  13. SenseWearMini and Actigraph GT3X Accelerometer Classification of Observed Sedentary and Light-Intensity Physical Activities in a Laboratory Setting.

    PubMed

    Feehan, Lynne M; Goldsmith, Charles H; Leung, April Y F; Li, Linda C

    2016-01-01

    Purpose: To compare the ability of SenseWear Mini (SWm) and Actigraph GT3X (AG3) accelerometers to differentiate between healthy adults' observed sedentary and light activities in a laboratory setting. Methods: The 22 participants (15 women, 7 men), ages 19 to 72 years, wore SWm and AG3 monitors and performed five sedentary and four light activities for 5 minutes each while observed in a laboratory setting. Performance was examined through comparisons of accuracy, sensitivity, specificity, positive and negative predictive values, and positive and negative likelihood ratios. Correct identification of both types of activities was examined using area under the receiver operating characteristic curve (AUC). Results: Both monitors demonstrated excellent ability to identify sedentary activities (sensitivity>0.89). The SWm monitor was better at identifying light activities (specificity 0.61-0.71) than the AG3 monitor (specificity 0.27-0.47) and thus also showed a greater ability to correctly identify both sedentary and light activities (SWm AUC 0.84; AG3 AUC 0.62-0.73). Conclusions: SWm may be a more suitable monitor for detecting time spent in sedentary and light-intensity activities. This finding has clinical and research relevance for evaluation of time spent in lower intensity physical activities by sedentary adults.

  14. Pb2+-induced toxicity is associated with p53-independent apoptosis and enhanced by glutamate in GT1-7 neurons.

    PubMed

    Loikkanen, Jarkko; Chvalova, Katerina; Naarala, Jonne; Vähäkangas, Kirsi H; Savolainen, Kai M

    2003-09-30

    Recent studies indicate that the glutamatergic neurotransmitter system is involved in neurotoxicity caused by inorganic lead (Pb2+). We studied the role of apoptosis in the effects induced by Pb2+ (0.01-100 microM) and glutamate (0.1 and 1 mM) in mouse hypothalamic GT1-7 neurons. Although glutamate alone had no effect on cell viability, it enhanced neuronal cell death induced by Pb2+ (1-100 microM) within 72 h. Glutamate alone neither induced caspase-3-like protease activity nor promoted internucleosomal DNA fragmentation, both biochemical hallmarks of apoptosis. However, concurrent exposure to Pb2+ (10 or 100 microM) and glutamate (1 mM) resulted in more prominent cleavage of the fluorogenic caspase-3 substrate (Ac-DEVD-AMC) than caused by the same Pb2+ concentrations alone at 24-72 h. The highest caspase-3-like protease activities were measured at 48 h. Internucleosomal DNA fragmentation caused by Pb2+ (10 or 100 microM) alone or together with glutamate (1 mM) was evident at 96 h, less clear at 72 h and absent at 48 h. Immunoblotting did not reveal any changes in p53 protein levels in cells exposed to Pb2+, glutamate or their combination at any studied time point (3-72 h). Our results suggest that Pb2+-induced neurotoxicity may partially be mediated through p53-independent apoptosis and enhanced by glutamate.

  15. Detection of feral GT73 transgenic oilseed rape (Brassica napus) along railway lines on entry routes to oilseed factories in Switzerland.

    PubMed

    Hecht, Mirco; Oehen, Bernadette; Schulze, Jürg; Brodmann, Peter; Bagutti, Claudia

    2014-01-01

    To obtain a reference status prior to cultivation of genetically modified oilseed rape (OSR, Brassica napus L.) in Switzerland, the occurrence of feral OSR was monitored along transportation routes and at processing sites. The focus was set on the detection of (transgenic) OSR along railway lines from the Swiss borders with Italy and France to the respective oilseed processing factories in Southern and Northern Switzerland (Ticino and region of Basel). A monitoring concept was developed to identify sites of largest risk of escape of genetically modified plants into the environment in Switzerland. Transport spillage of OSR seeds from railway goods cars particularly at risk hot spots such as switch yards and (un)loading points but also incidental and continuous spillage were considered. All OSR plants, including their hybridization partners which were collected at the respective monitoring sites were analyzed for the presence of transgenes by real-time PCR. On sampling lengths each of 4.2 and 5.7 km, respectively, 461 and 1,574 plants were sampled in Ticino and the region of Basel. OSR plants were found most frequently along the routes to the oilseed facilities, and in larger amounts on risk hot spots compared to sites of random sampling. At three locations in both monitored regions, transgenic B. napus line GT73 carrying the glyphosate resistance transgenes gox and CP4 epsps were detected (Ticino, 22 plants; in the region of Basel, 159).

  16. Endothelial Nitric Oxide Synthase (-786T>C) and Endothelin-1 (5665G>T) Gene Polymorphisms as Vascular Dysfunction Risk Factors in Sickle Cell Anemia.

    PubMed

    Vilas-Boas, Wendell; Figueiredo, Camylla V B; Pitanga, Thassila N; Carvalho, Magda O S; Santiago, Rayra P; Santana, Sânzio S; Guarda, Caroline C; Zanette, Angela M D; Cerqueira, Bruno A V; Gonçalves, Marilda S

    2016-01-01

    Sickle cell anemia (SCA) patients have vascular complications, and polymorphisms in endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) genes were associated with ET-1 and nitric oxide disturbance. We investigate the association of ET-1 5665G>T and eNOS -786T>C polymorphisms with soluble adhesion molecules (sVCAM-1 and sICAM-1), biochemical markers, and medical history. We studied 101 SCA patients; carriers of eNOS minor allele (C) had the highest levels of sVCAM-1, and carriers of ET-1 minor allele had more occurrence of acute chest syndrome (ACS). The multivariate analysis suggested the influence of the ET-1 gene on ACS outcome and an association of the eNOS gene with upper respiratory tract infection. We suggest that eNOS and ET-1 gene polymorphisms can influence SCA pathophysiology and that eNOS variant in SCA patients might be important to nitric oxide activity and vascular alteration. We found an association of the ET-1 minor allele in ACS, showing the importance of genetic screening in SCA.

  17. An In Vitro System Comprising Immortalized Hypothalamic Neuronal Cells (GT1–7 Cells) for Evaluation of the Neuroendocrine Effects of Essential Oils

    PubMed Central

    Mizuno, Dai; Konoha-Mizuno, Keiko; Mori, Miwako; Yamazaki, Kentaro; Haneda, Toshihiro; Koyama, Hironari; Kawahara, Masahiro

    2015-01-01

    Aromatherapy and plant-based essential oils are widely used as complementary and alternative therapies for symptoms including anxiety. Furthermore, it was reportedly effective for the care of several diseases such as Alzheimer's disease and depressive illness. To investigate the pharmacological effects of essential oils, we developed an in vitro assay system using immortalized hypothalamic neuronal cells (GT1–7 cells). In this study, we evaluated the effects of essential oils on neuronal death induced by hydrogen peroxide (H2O2), aluminum, zinc, or the antagonist of estrogen receptor (tamoxifen). Among tests of various essential oils, we found that H2O2-induced neuronal death was attenuated by the essential oils of damask rose, eucalyptus, fennel, geranium, ginger, kabosu, mandarin, myrrh, and neroli. Damask rose oil had protective effects against aluminum-induced neurotoxicity, while geranium and rosemary oil showed protective activity against zinc-induced neurotoxicity. In contrast, geranium oil and ginger oil enhanced the neurotoxicity of tamoxifen. Our in vitro assay system could be useful for the neuropharmacological and endocrine pharmacological studies of essential oils. PMID:26576190

  18. Serotonin stimulates GnRH secretion through the c-Src-PLC gamma1 pathway in GT1-7 hypothalamic cells.

    PubMed

    Kim, Hyeon Soo; Yumkham, Sanatombi; Choi, Jang Hyun; Son, Gi Hoon; Kim, Kyungjin; Ryu, Sung Ho; Suh, Pann-Ghill

    2006-09-01

    Serotonin is a neurotransmitter that alters the hypothalamic-pituitary-adrenal axis. To date, however, the molecular mechanisms underlying the role of serotonin in hormone secretion have remained largely unclear. In this study, we report that serotonin activates phospholipase C (PLC) gamma1 in an Src-dependent manner in hypothalamic GT1-7 cells, and that pretreatment with either 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazole [3, 4-d] pyrimidine, an Src-kinase inhibitor, or U73122, a PLC inhibitor, attenuates the serotonin-induced increase in calcium levels. Also, PLC gamma1 binds to c-Src through the Src-homology (SH) 223 domain upon serotonin treatment. Moreover, calcium increase is alleviated in the cells transientlyexpressing SH223 domain-deleted PLC gamma1 or lipase inactive mutant PLC gamma1, as compared with cells transfected with wild-type PLC gamma1. Furthermore, the inhibition of the activities of either PLC or Src results in a significant diminution of the serotonin-induced release of gonadotropin-releasing hormone (GnRH). In addition, the results of our small-interfering RNA experiment confirm that endogenous PLC gamma1 is a prerequisite for serotonin-mediated signaling pathways. Taken together, our findings demonstrate that serotonin stimulates the release of GnRH through the Src-PLC gamma1 pathway, via the modulation of intracellular calcium levels.

  19. Endothelial Nitric Oxide Synthase (−786T>C) and Endothelin-1 (5665G>T) Gene Polymorphisms as Vascular Dysfunction Risk Factors in Sickle Cell Anemia

    PubMed Central

    Vilas-Boas, Wendell; Figueiredo, Camylla V. B.; Pitanga, Thassila N.; Carvalho, Magda O. S.; Santiago, Rayra P.; Santana, Sânzio S.; Guarda, Caroline C.; Zanette, Angela M. D.; Cerqueira, Bruno A. V.; Gonçalves, Marilda S.

    2016-01-01

    Sickle cell anemia (SCA) patients have vascular complications, and polymorphisms in endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) genes were associated with ET-1 and nitric oxide disturbance. We investigate the association of ET-1 5665G>T and eNOS −786T>C polymorphisms with soluble adhesion molecules (sVCAM-1 and sICAM-1), biochemical markers, and medical history. We studied 101 SCA patients; carriers of eNOS minor allele (C) had the highest levels of sVCAM-1, and carriers of ET-1 minor allele had more occurrence of acute chest syndrome (ACS). The multivariate analysis suggested the influence of the ET-1 gene on ACS outcome and an association of the eNOS gene with upper respiratory tract infection. We suggest that eNOS and ET-1 gene polymorphisms can influence SCA pathophysiology and that eNOS variant in SCA patients might be important to nitric oxide activity and vascular alteration. We found an association of the ET-1 minor allele in ACS, showing the importance of genetic screening in SCA. PMID:27486304

  20. A Novel Missense Mutation 224G>T (R75M) in SRY Coding Region Interferes with Nuclear Import and Results in 46, XY Complete Gonadal Dysgenesis

    PubMed Central

    He, Shanshan; Zhang, Tengfei; Yin, Chenxing; Chen, Yunping; Zheng, Shuqi; Zhang, Jixia; Li, Lin

    2016-01-01

    SRY-mutation-caused sex reversal is a rare disease and mostly associated with a de novo mutation since the patients with defective SRY is infertile. There are many reports about SRY-mutation associated 46, XY ovarian disorder of sex development (DSD), but few described their molecular mechanism. Here we report a de novo mutation 224G>T (R75M) in SRY associated with a phenotypic female, 46, XY karyotype and dysgerminoma. The wild and mutated SRY were cloned into recombinant plasmid and expressed in cells in vitro, the result showed the mutated SRY is greatly accumulated in cytoplasm while the wild type SRY is mostly localized in nucleus. To make sure no other genes were involved, we performed the trio-based whole exome sequencing using the DNA samples from the proband and the parents, and no mutations were identified especially in DHH, NR0B1, NR5A1, SOX9 and MAP3K1, indicating the de novo mutation in SRY is the single defect responsible for the female sex reversal. We also used bioinformatics simulation analysis to predict impact of the mutation on SRY function, and find the R75 in wild type SRY can form a hydrogen bond with serine at 91 (S91) that make the SRY protein well fit into the minor groove of target DNA, while the M75 in the mutated SRY can’t. Finally, we reviewed SRY mutations based on the available references and analyzed the mutation distribution patterns according to density and continuity, which may be useful for further study of the SRY structure, function, and its relatedness with DSD. PMID:28030592

  1. A Novel Missense Mutation 224G>T (R75M) in SRY Coding Region Interferes with Nuclear Import and Results in 46, XY Complete Gonadal Dysgenesis.

    PubMed

    Fan, Wufang; Wang, Bei; He, Shanshan; Zhang, Tengfei; Yin, Chenxing; Chen, Yunping; Zheng, Shuqi; Zhang, Jixia; Li, Lin

    2016-01-01

    SRY-mutation-caused sex reversal is a rare disease and mostly associated with a de novo mutation since the patients with defective SRY is infertile. There are many reports about SRY-mutation associated 46, XY ovarian disorder of sex development (DSD), but few described their molecular mechanism. Here we report a de novo mutation 224G>T (R75M) in SRY associated with a phenotypic female, 46, XY karyotype and dysgerminoma. The wild and mutated SRY were cloned into recombinant plasmid and expressed in cells in vitro, the result showed the mutated SRY is greatly accumulated in cytoplasm while the wild type SRY is mostly localized in nucleus. To make sure no other genes were involved, we performed the trio-based whole exome sequencing using the DNA samples from the proband and the parents, and no mutations were identified especially in DHH, NR0B1, NR5A1, SOX9 and MAP3K1, indicating the de novo mutation in SRY is the single defect responsible for the female sex reversal. We also used bioinformatics simulation analysis to predict impact of the mutation on SRY function, and find the R75 in wild type SRY can form a hydrogen bond with serine at 91 (S91) that make the SRY protein well fit into the minor groove of target DNA, while the M75 in the mutated SRY can't. Finally, we reviewed SRY mutations based on the available references and analyzed the mutation distribution patterns according to density and continuity, which may be useful for further study of the SRY structure, function, and its relatedness with DSD.

  2. Integrated method for crater detection from topography and optical images and the new PH9224GT catalogue of Phobos impact craters

    NASA Astrophysics Data System (ADS)

    Salamunićcar, Goran; Lončarić, Sven; Pina, Pedro; Bandeira, Lourenço; Saraiva, José

    2014-06-01

    In a large majority of lunar and planetary surface images, impact craters are the most abundant geological features. Therefore, it is not surprising that crater detection algorithms (CDAs) are one of the most studied subjects of image processing and analysis in lunar and planetary science. In this work we are proposing an Integrated CDA, consisting of: (1) utilization of DEM (digital elevation map)-based CDA; (2) utilization of an optical-based CDA; (3) re-projection of used datasets and crater coordinates from normal to rotated view and back; (4) correction of the brightness and contrast of a used optical image; and (5) tile generation for the optical-based CDA and an assembling of results with an elimination of multiple detections, in combination with a pyramid approach down to the resolution of the available DEM image; and (6) a final integration of the results of DEM-based and optical-based CDAs, including a removal of duplicates. The proposed CDA is applied to one specific asteroid-like body, the small Martian moon Phobos. The experimental evaluation of the proposed CDA is done by a manual verification of crater-candidates and a search for uncatalogued craters. The evaluation has shown that the proposed CDA was used successfully for cataloging Phobos craters. The major result of this paper is the PH9224GT - currently the most complete global catalogue of the 9224 Phobos craters. The possible applications of the new catalogue are: (1) age estimations for any selected location; and (2) comparison/evaluation of the different chronology and production functions for Phobos. This confirms the practical applicability of the new Integrated CDA - an additional result of this paper, which can be used in order to considerably extend the current crater catalogues.

  3. Cell death mechanisms in GT1-7 GnRH cells exposed to polychlorinated biphenyls PCB74, PCB118, and PCB153

    SciTech Connect

    Dickerson, Sarah M.; Guevara, Esperanza; Woller, Michael J.; Gore, Andrea C.

    2009-06-01

    Exposure to endocrine disrupting chemicals (EDCs) such as polychlorinated biphenyls (PCBs) causes functional deficits in neuroendocrine systems. We used an immortalized hypothalamic GT1-7 cell line, which synthesizes the neuroendocrine peptide gonadotropin-releasing hormone (GnRH), to examine the neurotoxic and endocrine disrupting effects of PCBs and their mechanisms of action. Cells were treated for 1, 4, 8, or 24 h with a range of doses of a representative PCB from each of three classes: coplanar (2,4,4',5-tetrachlorobiphenyl: PCB74), dioxin-like coplanar (2',3,4,4',5' pentachlorobiphenyl: PCB118), non-coplanar (2,2',4,4',5,5'-hexachlorobiphenyl: PCB153), or their combination. GnRH peptide concentrations, cell viability, apoptotic and necrotic cell death, and caspase activation were quantified. In general, GnRH peptide levels were suppressed by high doses and longer durations of PCBs, and elevated at low doses and shorter timepoints. The suppression of GnRH peptide levels was partially reversed in cultures co-treated with the estrogen receptor antagonist ICI 182,780. All PCBs reduced viability and increased both apoptotic and necrotic cell death. Although the effects for the three classes of PCBs were often similar, subtle differences in responses, together with evidence that the combination of PCBs acted slightly different from individual PCBs, suggest that the three tested PCB compounds may act via slightly different or more than one mechanism. These results provide evidence that PCB congeners have endocrine disrupting and/or neurotoxic effects on the hypothalamic GnRH cell line, a finding that has implications for environmental endocrine disruption in animals.

  4. In-Silico Analysis of Binding Site Features and Substrate Selectivity in Plant Flavonoid-3-O Glycosyltransferases (F3GT) through Molecular Modeling, Docking and Dynamics Simulation Studies

    PubMed Central

    Sharma, Ranu; Panigrahi, Priyabrata; Suresh, C.G.

    2014-01-01

    Flavonoids are a class of plant secondary metabolites that act as storage molecules, chemical messengers, as well as participate in homeostasis and defense processes. They possess pharmaceutical properties important for cancer treatment such as antioxidant and anti-tumor activities. The drug-related properties of flavonoids can be improved by glycosylation. The enzymes glycosyltransferases (GTs) glycosylate acceptor molecules in a regiospecific manner with the help of nucleotide sugar donor molecules. Several plant GTs have been characterized and their amino acid sequences determined. However, three-dimensional structures of only a few are reported. Here, phylogenetic analysis using amino acid sequences have identified a group of GTs with the same regiospecific activity. The structures of these closely related GTs were modeled using homologous GT structures. Their substrate binding sites were elaborated by docking flavonoid acceptor and UDP-sugar donor molecules in the modeled structures. Eight regions near the acceptor binding site in the N- and C- terminal domain of GTs have been identified that bind and specifically glycosylate the 3-OH group of acceptor flavonoids. Similarly, a conserved motif in the C-terminal domain is known to bind a sugar donor substrate. In certain GTs, the substitution of a specific glutamine by histidine in this domain changes the preference of sugar from glucose to galactose as a result of changed pattern of interactions. The molecular modeling, docking, and molecular dynamics simulation studies have revealed the chemical and topological features of the binding site and thus provided insights into the basis of acceptor and donor recognition by GTs. PMID:24667893

  5. G/T Substitution in Intron 1 of the UNC13B Gene Is Associated With Increased Risk of Nephropathy in Patients With Type 1 Diabetes

    PubMed Central

    Trégouet, David-Alexandre; Groop, Per-Henrik; McGinn, Steven; Forsblom, Carol; Hadjadj, Samy; Marre, Michel; Parving, Hans-Henrik; Tarnow, Lise; Telgmann, Ralph; Godefroy, Tiphaine; Nicaud, Viviane; Rousseau, Rachel; Parkkonen, Maikki; Hoverfält, Anna; Gut, Ivo; Heath, Simon; Matsuda, Fumihiko; Cox, Roger; Kazeem, Gbenga; Farrall, Martin; Gauguier, Dominique; Brand-Herrmann, Stefan-Martin; Cambien, François; Lathrop, Mark; Vionnet, Nathalie

    2008-01-01

    OBJECTIVE— Genetic and environmental factors modulate the susceptibility to diabetic nephropathy, as initiating and/or progression factors. The objective of the European Rational Approach for the Genetics of Diabetic Complications (EURAGEDIC) study is to identify nephropathy susceptibility genes. We report molecular genetic studies for 127 candidate genes for nephropathy. RESEARCH DESIGN AND METHODS— Polymorphisms were identified through sequencing of promoter, exon, and flanking intron gene regions and a database search. A total of 344 nonredundant SNPs and nonsynonymous variants were tested for association with diabetic nephropathy (persistent albuminuria ≥300 mg/24 h) in a large type 1 diabetes case/control (1,176/1,323) study from three European populations. RESULTS— Only one SNP, rs2281999, located in the UNC13B gene, was significantly associated with nephropathy after correction for multiple testing. Analyses of 21 additional markers fully characterizing the haplotypic variability of the UNC13B gene showed consistent association of SNP rs13293564 (G/T) located in intron 1 of the gene with nephropathy in the three populations. The odds ratio (OR) for nephropathy associated with the TT genotype was 1.68 (95% CI 1.29–2.19) (P = 1.0 × 10−4). This association was replicated in an independent population of 412 case subjects and 614 control subjects (combined OR of 1.63 [95% CI 1.30–2.05], P = 2.3 × 10−5). CONCLUSIONS— We identified a polymorphism in the UNC13B gene associated with nephropathy. UNC13B mediates apopotosis in glomerular cells in the presence of hyperglycemia, an event occurring early in the development of nephropathy. We propose that this polymorphism could be a marker for the initiation of nephropathy. However, further studies are needed to clarify the role of UNC13B in nephropathy. PMID:18633107

  6. The mtDNA nt7778 G/T polymorphism augments formation of lymphocytic foci but does not aggravate cerulein-induced acute pancreatitis in mice.

    PubMed

    Müller, Sarah; Krüger, Burkhard; Lange, Falko; Bock, Cristin N; Nizze, Horst; Glass, Änne; Ibrahim, Saleh M; Jaster, Robert

    2014-01-01

    A polymorphism in the ATP synthase 8 (ATP8) gene of the murine mitochondrial genome, G-to-T transversion at position 7778, has been suggested to increase susceptibility to multiple autoimmune diseases, including autoimmune pancreatitis (AIP). The polymorphism also induces mitochondrial reactive oxygen species generation, secretory dysfunction and β-cell mass adaptation. Here, we have used two conplastic mouse strains, C57BL/6N-mtAKR/J (B6-mtAKR; nt7778 G; control) and C57BL/6N-mtFVB/N (B6-mtFVB; nt7778 T), to address the question if the polymorphism also affects the course of cerulein-induced acute pancreatitis in mice. Therefore, two age groups of mice (3 and 12-month-old, respectively) were subjected to up to 7 injections of the secretagogue cerulein (50 µg/kg body weight) at hourly intervals. Disease severity was assessed at time points from 3 hours to 7 days based on pancreatic histopathology, serum levels of α-amylase and activities of myeloperoxidase (MPO) in lung tissue. A comparison of cerulein-induced pancreatic tissue damage and increases of α-amylase and MPO activities showed no differences between the age-matched groups of both strains. Interestingly, histological evaluation of pancreatic tissue of both untreated and cerulein-treated B6-mtAKR and B6-mtFVB mice also revealed the presence of infiltrates of immune cells surrounding ducts and vessels; a finding that is compatible with an early stage of AIP. After recovery from cerulein-induced pancreatitis (day 7 after the injections), 12-month-old B6-mtFVB mice but not B6-mtAKR mice displayed aggravated lymphocytic lesions. A comparison of 12-month-old mice with other age groups of both strains revealed that lymphocytic foci were largely absent in 3-month-old mice, while 24-month-old mice were more affected. Together, our data suggest that the mtDNA nt7778 G/T polymorphism does not aggravate cerulein-induced acute pancreatitis. Autoimmune-like lesions, however, may progress faster if additional tissue

  7. Calibration and Cross-Validation of the ActiGraph wGT3X+ Accelerometer for the Estimation of Physical Activity Intensity in Children with Intellectual Disabilities

    PubMed Central

    McGarty, Arlene M.; Penpraze, Victoria; Melville, Craig A.

    2016-01-01

    Background Valid objective measurement is integral to increasing our understanding of physical activity and sedentary behaviours. However, no population-specific cut points have been calibrated for children with intellectual disabilities. Therefore, this study aimed to calibrate and cross-validate the first population-specific accelerometer intensity cut points for children with intellectual disabilities. Methods Fifty children with intellectual disabilities were randomly assigned to the calibration (n = 36; boys = 28, 9.53±1.08yrs) or cross-validation (n = 14; boys = 9, 9.57±1.16yrs) group. Participants completed a semi-structured school-based activity session, which included various activities ranging from sedentary to vigorous intensity. Direct observation (SOFIT tool) was used to calibrate the ActiGraph wGT3X+, which participants wore on the right hip. Receiver Operating Characteristic curve analyses determined the optimal cut points for sedentary, moderate, and vigorous intensity activity for the vertical axis and vector magnitude. Classification agreement was investigated using sensitivity, specificity, total agreement, and Cohen’s kappa scores against the criterion measure of SOFIT. Results The optimal (AUC = .87−.94) vertical axis cut points (cpm) were ≤507 (sedentary), 1008−2300 (moderate), and ≥2301 (vigorous), which demonstrated high sensitivity (81−88%) and specificity (81−85%). The optimal (AUC = .86−.92) vector magnitude cut points (cpm) of ≤1863 (sedentary), 2610−4214 (moderate), and ≥4215 (vigorous) demonstrated comparable, albeit marginally lower, accuracy than the vertical axis cut points (sensitivity = 80−86%; specificity = 77−82%). Classification agreement ranged from moderate to almost perfect (κ = .51−.85) with high sensitivity and specificity, and confirmed the trend that accuracy increased with intensity, and vertical axis cut points provide higher classification agreement than vector magnitude cut points

  8. Effects of PSCA rs2294008 (C/T) and c-MYC rs9642880 (G/T) polymorphisms on bladder cancer: evidence from a meta-analysis

    PubMed Central

    Gao, Jie; Yang, Peng-Tao; Diao, Yan; Kang, Hua-Feng; Zhao, Yang; Lin, Shuai; Wang, Zi-Ming; Wang, Meng; Wang, Xi-Jing; Dai, Zhi-Jun

    2015-01-01

    Previous studies have investigated the associations between the two polymorphisms (prostate stem cell antigen (PSCA) rs2294008 C/T and c-MYC rs9642880 G/T) and bladder cancer (BC) risk. However, the results are inconsistent. We therefore carried out a meta-analysis to estimate the relationship between PSCA/c-MYC polymorphisms and BC risk. We searched PubMed up to November 2014 to identify potentially eligible literatures. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to estimate the strength of the associations, the data were further stratified by ethnicity. Heterogeneity was evaluated by Q test and I2 statistics. Begg’s funnel plot and Egger’s test were used to assess the publication bias. 11 studies from 9 articles were identified, including a total of 16,814 cancer cases and 52,868 case-free controls. We found a significant association between PSCA rs2294008 polymorphism and BC risk (the allele contrast model: OR = 1.14, 95% CI = 1.11-1.18; homozygote comparison: OR = 1.28, 95% CI = 1.20-1.37; heterozygote comparison: OR = 1.23, 95% CI = 1.17-1.30; dominant model: OR = 1.25, 95% CI = 1.19-1.31 and recessive model: OR = 1.13, 95% CI = 1.07-1.20). Moreover, a significant increased risk of BC was confirmed both in Caucasian and in Asians. For c-MYC rs9642880 polymorphism, significant increased BC risk was detected under the following genetic models (the allele contrast model: OR = 1.20, 95% CI = 1.13-1.27; homozygote comparison: OR = 1.37, 95% CI = 1.21-1.55; heterozygote comparison: OR = 1.20, 95% CI = 1.09-1.32; dominant model: OR = 1.25, 95% CI = 1.14-1.37 and recessive model: OR = 1.26, 95% CI = 1.13-1.40). Further stratified analysis by ethnicity also observed the same results. This meta-analysis suggested that PSCA rs2294008 and c-MYC rs9642880 polymorphisms may increase the BC risk. Further studies are needed to clarify the effects. PMID:25932146

  9. 48 CFR 915.404-4-71-4 - Considerations affecting fee amounts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... and transformer stations; water treatment plants; sewage disposal plants; hospitals; and ordinary..., railroads, grading, sewers, storm drains, and water and power distribution systems. (4) Class...

  10. 48 CFR 915.404-4-71-4 - Considerations affecting fee amounts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... and transformer stations; water treatment plants; sewage disposal plants; hospitals; and ordinary..., railroads, grading, sewers, storm drains, and water and power distribution systems. (4) Class...

  11. 48 CFR 915.404-4-71-4 - Considerations affecting fee amounts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... and transformer stations; water treatment plants; sewage disposal plants; hospitals; and ordinary..., railroads, grading, sewers, storm drains, and water and power distribution systems. (4) Class...

  12. 48 CFR 915.404-4-71-4 - Considerations affecting fee amounts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... and transformer stations; water treatment plants; sewage disposal plants; hospitals; and ordinary..., railroads, grading, sewers, storm drains, and water and power distribution systems. (4) Class...

  13. 48 CFR 915.404-4-71-4 - Considerations affecting fee amounts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... and transformer stations; water treatment plants; sewage disposal plants; hospitals; and ordinary..., railroads, grading, sewers, storm drains, and water and power distribution systems. (4) Class...

  14. 48 CFR 915.404-4-71-3 - Factors for determining fees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) The relative difficulty of work, including consideration of technical and administrative knowledge, skill, experience and clarity of technical specifications; (4) Degree and amount of contract...

  15. 48 CFR 27.404-4 - Contractor's release, publication, and use of data.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... statutes. However, agencies may restrict the release or disclosure of computer software that is or is... software for purposes of established agency distribution programs, or where required to accomplish the purpose for which the software is acquired. (b) Except for the results of basic or applied research...

  16. 48 CFR 915.404-4-72 - Special considerations for cost-plus-award-fee contracts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915...%. However, in the event the base fee is to be less than 50% of the fixed fee, the maximum potential award... potential award fees shall apply in award fee contracts: (percent is stated as percent of fee...

  17. 48 CFR 27.404-4 - Contractor's release, publication, and use of data.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... reporting on the results of unclassified basic or applied research, except as provided in applicable U.S... applied research. Agencies may also preclude a contractor from asserting copyright in any computer... purpose for which the software is acquired. (b) Except for the results of basic or applied research...

  18. Comparison between Mother, ActiGraph wGT3X-BT, and a hand tally for measuring steps at various walking speeds under controlled conditions

    PubMed Central

    Rathleff, Camilla Rams; Kalstrup, Pernille Møller; Madsen, Niels Kragh; Pedersen, Elena Selmar; Pape-Haugaard, Louise Bilenberg; Villumsen, Morten

    2016-01-01

    Introduction Walking is endorsed as health enhancing and is the most common type of physical activity among older adults. Accelerometers are superior to self-reports when measuring steps, however, if they are to be used by clinicians the validity is of great importance. The aim of this study was to investigate the criterion validity of Mother and ActiGraph wGT3X-BT in measuring steps by comparing the devices to a hand tally under controlled conditions in healthy participants. Methods Thirty healthy participants were fitted with a belt containing the sensor of Mother (Motion Cookie) and ActiGraph. Participants walked on a treadmill for two minutes at each of the following speeds; 3.2, 4.8, and 6.4 km/h. The treadmill walking was video recorded and actual steps were subsequently determined by using a hand tally. Wilcoxon’s signed ranks test was used to determine whether Mother and ActiGraph measured an identical number of steps compared to the hand tally. Intraclass correlation coefficients were calculated to determine the relationship and Root Mean Square error was calculated to investigate the average error between the devices and the hand tally. Percent differences (PD) were calculated for between-instrument agreement (Mother vs. the hand tally and ActiGraph vs. the hand tally) and PDs below 3% were interpreted as acceptable and clinically irrelevant. Results Mother and ActiGraph under-counted steps significantly compared to the hand tally at all walking speeds (p < 0.001). Mother had a median of total differences of 9.5 steps (IQR = 10) and ActiGraph 59 steps (IQR = 77). Mother had smaller PDs at all speeds especially at 3.2 km/h (2.5% compared to 26.7%). Mother showed excellent ICC values ≥0.88 (0.51–0.96) at all speeds whilst ActiGraph had poor and fair to good ICC values ranging from 0.03 (−0.09–0.21) at a speed of 3.2 km/h to 0.64 (0.16–0.84) at a speed of 6.4 km/h. Conclusion Mother provides valid measures of steps at walking speeds of 3.2, 4

  19. Mutagenesis of 8-oxoguanine adjacent to an abasic site in simian kidney cells: tandem mutations and enhancement of G-->T transversions.

    PubMed

    Kalam, M Abul; Basu, Ashis K

    2005-08-01

    Clustered DNA damages are well-established characteristics of ionizing radiation. As a model clustered lesion in the same strand of DNA, we have evaluated the mutagenic potential of 8-oxoguanine (8-oxoG) adjacent to a uracil in simian kidney cells using a phagemid vector. The uracil residue would be excised by the enzyme uracil DNA glycosylase in vivo generating an abasic site (AP site). A solitary uracil in either GUGTC or GTGUC sequence context provided >60% progeny containing GTGTC indicating that dAMP incorporation opposite the AP site or uracil occurred, but a >30% population showed replacement of U by A, C, or G, which suggests that dTMP, dGMP, or dCMP incorporation also occurred, respectively, opposite the AP site. While the preference for targeted base substitutions at the GUG site was T > C > A > G, the same at the GUC site was T > A > C > G. We conclude that base incorporation opposite an AP site is sequence-dependent. For 8-oxoG, as compared to 23-24% G-->T mutants from a single 8-oxoG in a TG(8-oxo)T sequence context, the tandem lesions UG(8-oxo)T and TG(8-oxo)U generated approximately 60 and >85% progeny, respectively, that did not contain the TGT sequence. A significant fraction of tandem mutations were detected when uracil was adjacent to 8-oxoG. What we found most interesting is that the total targeted G(8-oxo)-->T transversions that included both single and tandem mutations at the TG(8-oxo)U site was nearly 60% relative to about 30% at the UG(8-oxo)T site. A higher mutational frequency at the TG(8-oxo)U sequence may arise from a change in DNA polymerase that is more error prone. Thermal melting experiments showed that the Tm for the 8-oxoG:C pair in the TG(8-oxo)(AP*) sequence in a 12-mer was lower than the same in a (AP*)G(8-oxo)T 12-mer with deltadeltaG 0.8 kcal/mol (where AP* represents tetrahydrofuran, the model abasic site). When the 8-oxoG:C pair in each sequence was compared with a 8-oxoG:A pair, the former was found to be more stable than

  20. Analysis of aberrant splicing and nonsense-mediated decay of the stop codon mutations c.109G>T and c.504_505delCT in 7 patients with HMG-CoA lyase deficiency.

    PubMed

    Puisac, Beatriz; Teresa-Rodrigo, María Esperanza; Arnedo, María; Gil-Rodríguez, María Concepción; Pérez-Cerdá, Celia; Ribes, Antonia; Pié, Angeles; Bueno, Gloria; Gómez-Puertas, Paulino; Pié, Juan

    2013-04-01

    Eukaryotic cells can be protected against mutations that generate stop codons by nonsense-mediated mRNA decay (NMD) and/or nonsense-associated altered splicing (NAS). However, the processes are only partially understood and do not always occur. In this work, we study these phenomena in the stop codon mutations c.109G>T (p.Glu37*) and c.504_505delCT; the second and third most frequent mutations in HMG-CoA lyase deficiency (MIM #246450). The deficiency affects the synthesis of ketone bodies and produces severe disorders during early childhood. We used a minigene approach, real-time quantitative PCR and the inhibition of NMD by puromycin treatment, to study the effect of stop codons on splicing (NAS) and NMD in seven patients. Surprisingly, none of the stop codons studied appears to be the direct cause of aberrant splicing. In the mutation c.109G>T, the splicing is due to the base change G>T at position 109, which is critical and cannot be explained by disruption of exonic splicing enhancer (ESE) elements, by the appearance of exonic splicing silencer (ESS) elements which were predicted by bioinformatic tools or by the stop codons. Moreover, the mutation c.504_505delCT produces two mRNA transcripts both with stop codons that generate simultaneous NMD phenomena. The effects of the mutations studied on splicing seemed to be similar in all the patients. Furthermore, we report a Spanish patient with 3-hydroxy-3-methylglutaric aciduria and a novel missense mutation: c.825C>G (p.Asn275Lys).

  1. Lack of association between interleukin 28B gene polymorphisms (rs8099917G/T, rs12979860 C/T) and susceptibility to chronic hepatitis C virus infection, Tehran, Iran

    PubMed Central

    Karkhane, Maryam; Mohebbi, Seyed Reza; Azimzadeh, Pedram; Saeedi Niasar, Mahsa; Sarbazi, Mohamad Reza; Sharifian, Afsaneh; Alizadeh, Afshin Mohammad

    2016-01-01

    Aim: Chronic Hepatitis C infection is a critical health problem worldwide, which caused by hepatitis C virus (HCV). Interleukin 28B (IL28B) is a determinant factor in disease progression and also susceptibility to chronic HCV infection. Background: The most significant aim of this study is to analyze the association between IL28B gene polymorphisms with susceptibility to chronic HCV infection in Iranian population. Methods: This study follows a case- control study design, in which, 288 patients with chronic hepatitis C and 250 healthy individuals were genotyped for IL28B polymorphisms (rs12979860 C/T and rs8099917 G/T). Studied population collected from Taleghani Haospital, Tehran. Genotyping of IL28B gene polymorphisms were performed using PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) method. 10 percent of the studied population was sequenced to validate the results. Results: rs8099917 G/T and rs12979860 C/T were differently distributed in hepatitis C patients and healthy controls in the female gender. TT, TG and GG genotypes distribution in the female gender were 56.7%, 39.8% and 4.5% in cases and 67%, 31.6% and 1.4% in controls (p=0.54). Also CC, CT and TT genotypes distribution were 31.8%, 61.4% and 6.8% in cases and 51.7%, 44.9% and 3.4% in controls (p=0.2). However, there was no significant difference in the allelic frequency and genotype distribution of rs12979860 C/T and rs8099917 T/G in both HCV patients with genotype 1a and 3a. Conclusion: It seems that rs8099917 G/T polymorphism plays a significant role in susceptibility to chronic HCV infection in Iranian population. On the other hand, no association was found between rs12979860 C/T polymorphisms and chronic hepatitis C. PMID:28224025

  2. 17beta-estradiol at physiological concentrations augments Ca(2+) -activated K+ currents via estrogen receptor beta in the gonadotropin-releasing hormone neuronal cell line GT1-7.

    PubMed

    Nishimura, Ichiro; Ui-Tei, Kumiko; Saigo, Kaoru; Ishii, Hirotaka; Sakuma, Yasuo; Kato, Masakatsu

    2008-02-01

    Estrogens play essential roles in the neuroendocrine control of reproduction. In the present study, we focused on the effects of 17beta-estradiol (E2) on the K(+) currents that regulate neuronal cell excitability and carried out perforated patch-clamp experiments with the GnRH-secreting neuronal cell line GT1-7. We revealed that a 3-d incubation with E2 at physiological concentrations (100 pm to 1 nm) augmented Ca(2+)-activated K(+) [K(Ca)] currents without influencing Ca(2+)-insensitive voltage-gated K(+) currents in GT1-7 cells. Acute application of E2 (1 nm) had no effect on the either type of K(+) current. The augmentation was completely blocked by an estrogen receptor (ER) antagonist, ICI-182,780. An ERbeta-selective agonist, 2,3-bis(4-hydroxyphenyl)-propionitrile, augmented the K(Ca) currents, although an ERalpha-selective agonist, 4,4',4''-[4-propyl-(1H)-pyrazole-1,3,5-triyl]tris-phenol, had no effect. Knockdown of ERbeta by means of RNA interference blocked the effect of E2 on the K(Ca) currents. Furthermore, semiquantitative RT-PCR analysis revealed that the levels of BK channel subunit mRNAs for alpha and beta4 were significantly increased by incubating cells with 300 pm E2 for 3 d. In conclusion, E2 at physiological concentrations augments K(Ca) currents through ERbeta in the GT1-7 GnRH neuronal cell line and increases the expression of the BK channel subunit mRNAs, alpha and beta4.

  3. Reproductive Physiology in Young Men Is Cumulatively Affected by FSH-Action Modulating Genetic Variants: FSHR -29G/A and c.2039 A/G, FSHB -211G/T

    PubMed Central

    Grigorova, Marina; Punab, Margus; Punab, Anna Maria; Poolamets, Olev; Vihljajev, Vladimir; Žilaitienė, Birutė; Erenpreiss, Juris; Matulevičius, Valentinas; Laan, Maris

    2014-01-01

    Follicle-Stimulating Hormone Receptor (FSHR) -29G/A polymorphism (rs1394205) was reported to modulate gene expression and reproductive parameters in women, but data in men is limited. We aimed to bring evidence to the effect of FSHR -29G/A variants in men. In Baltic young male cohort (n = 982; Estonians, Latvians, Lithuanians; aged 20.2±2.0 years), the FSHR -29 A-allele was significantly associated with higher serum FSH (linear regression: effect 0.27 IU/L; P = 0.0019, resistant to Bonferroni correction for multiple testing) and showed a non-significant trend for association with higher LH (0.19 IU/L) and total testosterone (0.93 nmol/L), but reduced Inhibin B (−7.84 pg/mL) and total testes volume (effect −1.00 mL). Next, we extended the study and tested the effect of FSHR gene haplotypes determined by the allelic combination of FSHR -29G/A and a well-studied variant c.2039 A/G (Asn680Ser, exon 10). Among the FSHR -29A/2039G haplotype carriers (A-Ser; haplotype-based linear regression), this genetic effect was enhanced for FSH (effect 0.40 IU/L), Inhibin B (−16.57 pg/mL) and total testes volume (−2.34 mL). Finally, we estimated the total contribution of three known FSH-action modulating SNPs (FSHB -211G/T; FSHR -29G/A, c.2039 A/G) to phenotypic variance in reproductive parameters among young men. The major FSH-action modulating SNPs explained together 2.3%, 1.4%, 1.0 and 1.1% of the measured variance in serum FSH, Inhibin B, testosterone and total testes volume, respectively. In contrast to the young male cohort, neither FSHR -29G/A nor FSHR haplotypes appeared to systematically modulate the reproductive physiology of oligozoospermic idiopathic infertile patients (n = 641, Estonians; aged 31.5±6.0 years). In summary, this is the first study showing the significant effect of FSHR -29G/A on male serum FSH level. To account for the genetic effect of known common polymorphisms modulating FSH-action, we suggest haplotype-based analysis of FSHR SNPs

  4. Future chelation monotherapy and combination therapy strategies in thalassemia and other conditions. comparison of deferiprone, deferoxamine, ICL670, GT56-252, L1NAll and starch deferoxamine polymers.

    PubMed

    Kontoghiorghes, George J

    2006-01-01

    Deferiprone (L1), and appropriate combinations with deferoxamine (DFO), can be used effectively for the treatment of thalassemia and other transfusional iron loading conditions. A number of experimental iron chelators such as deferasirox or ICL670 or Exjade (4-(3,5-bis (2-hydroxyphenyl)-1,2,4-triazol-1-yl)-benzoic acid), deferitrin (4,5-dihydro-2-(2,4-dihydroxyphenyl)-4-methylthiazole-4 (S)-carboxylic acid) or GT56-252, 1-allyl-2-methyl-3-hydroxypyrid-4-one or L1NAll and starch DFO polymers, are under clinical evaluation. ICL670 is the most advanced in development and appears to be effective in reducing liver iron in some patients but is overall ineffective in causing negative iron balance. It is also suspected that it is not effective in cardiac iron removal. Combination therapies using L1, DFO and new iron chelating drugs may cause higher efficacy and lower toxicity by comparison to monotherapies. However, several limitations including the high cost of the new chelating drugs may not facilitate the availability of these new treatments to the vast majority of thalassemia patients, most of whom live in developing countries.

  5. HTGR-GT systems optimization studies

    SciTech Connect

    Kammerzell, L.L.; Read, J.W.

    1980-06-01

    The compatibility of the inherent features of the high-temperature gas-cooled reactor (HTGR) and the closed-cycle gas turbine combined into a power conversion system results in a plant with characteristics consistent with projected utility needs and national energy goals. These characteristics are: (1) plant siting flexibility; (2) high resource utilization; (3) low safety risks; (4) proliferation resistance; and (5) low occupational exposure for operating and maintenance personnel. System design and evaluation studies on dry-cooled intercooled and nonintercooled commercial plants in the 800-MW(e) to 1200-MW(e) size range are described, with emphasis on the sensitivity of plant design objectives to variation of component and plant design parameters. The impact of these parameters on fuel cycle, fission product release, total plant economics, sensitivity to escalation rates, and plant capacity factors is examined.

  6. King County Metro Transit: Allison Hybrid Electric Transit Bus Laboratory Testing

    SciTech Connect

    Hayes, R. R.; Williams, A.; Ireland, J.; Walkowicz, K.

    2006-09-01

    Paper summarizes chassis dynamometer testing of two 60-foot articulated transit buses, one conventional and one hybrid, at NREL's ReFUEL Laboratory. It includes experimental setup, test procedures, and results from vehicle testing performed at the NREL ReFUEL laboratory.

  7. Smoke Emission Tests on Series II and Series III Allison T56 Turboprop Engines

    DTIC Science & Technology

    1986-12-01

    shows schematically the ARL smoke measuring equipment. Engine exhaust _». Refrigerated water trap Stainless steel sample probe Heated sample line...a system based on ARP 1179A. Experience at ARL has confirmed one major criticism of condensed water effecting the filter paper. This was largely...overcome by ensuring that the stainless steel lines and filter luolder were maintained at a temperature of 70oC. The addition of a refrigerated water

  8. 17 CFR 404.4 - Records to be made and preserved by government securities brokers and dealers that are financial...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... national banks), 12 CFR part 208 (relating to state member banks of the Federal Reserve System) or 12 CFR part 344 (relating to state banks that are not members of the Federal Reserve System), or is a United States branch or agency of a foreign bank and complies with 12 CFR part 12 (for federally...

  9. 48 CFR 915.404-4-70-5 - Special considerations-contracts with nonprofit organizations (other than educational institutions).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-4-70-5 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT..., scientific, or educational purposes, of which no part of the net earnings inure to the benefit of any...

  10. 48 CFR 915.404-4-70-5 - Special considerations-contracts with nonprofit organizations (other than educational institutions).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...-4-70-5 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT..., scientific, or educational purposes, of which no part of the net earnings inure to the benefit of any...

  11. Efficacy of a 12-Week Simeprevir Plus Peginterferon/Ribavirin (PR) Regimen in Treatment-Naïve Patients with Hepatitis C Virus (HCV) Genotype 4 (GT4) Infection and Mild-To-Moderate Fibrosis Displaying Early On-Treatment Virologic Response

    PubMed Central

    Asselah, Tarik; Moreno, Christophe; Sarrazin, Christoph; Gschwantler, Michael; Foster, Graham R.; Craxí, Antonio; Buggisch, Peter; Sanai, Faisal; Bicer, Ceyhun; Lenz, Oliver; Van Dooren, Gino; Nalpas, Catherine; Lonjon-Domanec, Isabelle; Schlag, Michael; Buti, Maria

    2017-01-01

    Background HCV GT4 accounts for up to 20% of HCV infections worldwide. Simeprevir, given for 12 weeks as part of a 24- or 48-week combination regimen with PR is approved for the treatment of chronic HCV GT4 infection. Primary study objectives were assessment of efficacy and safety of simeprevir plus PR in treatment-naïve patients with HCV GT4 treated for 12 weeks. Primary efficacy outcome was sustained virologic response 12 weeks post-treatment (SVR12). Additional objectives included investigation of potential associations of rapid virologic response and baseline factors with SVR12. Methods This multicentre, open-label, single-arm study (NCT01846832) evaluated efficacy and safety of simeprevir plus PR in 67 patients with HCV GT4 infection. Patients were treatment-naïve, aged 18–70 years with METAVIR F0–F2 fibrosis. Patients with early virologic response (HCV RNA <25 IU/mL [detectable/undetectable in IL28B CC patients or undetectable in IL28B CT/TT patients] at Week 2 and undetectable at Weeks 4 and 8) were eligible to stop all treatment at the end of Week 12, otherwise PR therapy was continued to Week 24. Results Of 67 patients treated, 34 (51%) qualified for 12-week treatment including all but one patient with IL28B CC genotype (14/15). All patients in the 12-week group had undetectable HCV RNA at end of treatment, and 97% (33/34) achieved SVR12. No new safety signals with simeprevir plus PR were identified. The proportion of patients experiencing Grade 3–4 adverse events was lower in the 12-week group than in the 24-week group. Conclusions Our findings on simeprevir plus PR therapy shortened to 12 weeks in patients with HCV GT4 infection with favourable baseline characteristics and displaying early on-treatment virologic response are encouraging. No new safety signals were associated with simeprevir plus PR in this study. Trial Registration NCT01846832 PMID:28056030

  12. The Genotype of the Donor for the (GT)n Polymorphism in the Promoter/Enhancer of FOXP3 Is Associated with the Development of Severe Acute GVHD but Does Not Affect the GVL Effect after Myeloablative HLA-Identical Allogeneic Stem Cell Transplantation.

    PubMed

    Noriega, Víctor; Martínez-Laperche, Carolina; Buces, Elena; Pion, Marjorie; Sánchez-Hernández, Noemí; Martín-Antonio, Beatriz; Guillem, Vicent; Bosch-Vizcaya, Anna; Bento, Leyre; González-Rivera, Milagros; Balsalobre, Pascual; Kwon, Mi; Serrano, David; Gayoso, Jorge; de la Cámara, Rafael; Brunet, Salut; Rojas-Contreras, Rafael; Nieto, José B; Martínez, Carmen; Gónzalez, Marcos; Espigado, Ildefonso; Vallejo, Juan C; Sampol, Antonia; Jiménez-Velasco, Antonio; Urbano-Ispizua, Alvaro; Solano, Carlos; Gallardo, David; Díez-Martín, José L; Buño, Ismael

    2015-01-01

    The FOXP3 gene encodes for a protein (Foxp3) involved in the development and functional activity of regulatory T cells (CD4+/CD25+/Foxp3+), which exert regulatory and suppressive roles over the immune system. After allogeneic stem cell transplantation, regulatory T cells are known to mitigate graft versus host disease while probably maintaining a graft versus leukemia effect. Short alleles (≤(GT)15) for the (GT)n polymorphism in the promoter/enhancer of FOXP3 are associated with a higher expression of FOXP3, and hypothetically with an increase of regulatory T cell activity. This polymorphism has been related to the development of auto- or alloimmune conditions including type 1 diabetes or graft rejection in renal transplant recipients. However, its impact in the allo-transplant setting has not been analyzed. In the present study, which includes 252 myeloablative HLA-identical allo-transplants, multivariate analysis revealed a lower incidence of grade III-IV acute graft versus host disease (GVHD) in patients transplanted from donors harboring short alleles (OR = 0.26, CI 0.08-0.82, p = 0.021); without affecting chronic GVHD or graft versus leukemia effect, since cumulative incidence of relapse, event free survival and overall survival rates are similar in both groups of patients.

  13. The Genotype of the Donor for the (GT)n Polymorphism in the Promoter/Enhancer of FOXP3 Is Associated with the Development of Severe Acute GVHD but Does Not Affect the GVL Effect after Myeloablative HLA-Identical Allogeneic Stem Cell Transplantation

    PubMed Central

    Buces, Elena; Pion, Marjorie; Sánchez-Hernández, Noemí; Martín-Antonio, Beatriz; Guillem, Vicent; Bosch-Vizcaya, Anna; Bento, Leyre; González-Rivera, Milagros; Balsalobre, Pascual; Kwon, Mi; Serrano, David; Gayoso, Jorge; de la Cámara, Rafael; Brunet, Salut; Rojas-Contreras, Rafael; Nieto, José B.; Martínez, Carmen; Gónzalez, Marcos; Espigado, Ildefonso; Vallejo, Juan C.; Sampol, Antonia; Jiménez-Velasco, Antonio; Urbano-Ispizua, Alvaro; Solano, Carlos; Gallardo, David; Díez-Martín, José L.; Buño, Ismael

    2015-01-01

    The FOXP3 gene encodes for a protein (Foxp3) involved in the development and functional activity of regulatory T cells (CD4+/CD25+/Foxp3+), which exert regulatory and suppressive roles over the immune system. After allogeneic stem cell transplantation, regulatory T cells are known to mitigate graft versus host disease while probably maintaining a graft versus leukemia effect. Short alleles (≤(GT)15) for the (GT)n polymorphism in the promoter/enhancer of FOXP3 are associated with a higher expression of FOXP3, and hypothetically with an increase of regulatory T cell activity. This polymorphism has been related to the development of auto- or alloimmune conditions including type 1 diabetes or graft rejection in renal transplant recipients. However, its impact in the allo-transplant setting has not been analyzed. In the present study, which includes 252 myeloablative HLA-identical allo-transplants, multivariate analysis revealed a lower incidence of grade III-IV acute graft versus host disease (GVHD) in patients transplanted from donors harboring short alleles (OR = 0.26, CI 0.08–0.82, p = 0.021); without affecting chronic GVHD or graft versus leukemia effect, since cumulative incidence of relapse, event free survival and overall survival rates are similar in both groups of patients. PMID:26473355

  14. Molecular and comparative morphological analysis of central European parasitic flatworms of the superfamily Brachylaimoidea Allison, 1943 (Trematoda: Plagiorchiida).

    PubMed

    Heneberg, Petr; Sitko, Jiljí; Bizos, Jiří

    2016-04-01

    The Brachylaimoidea are digenean parasites of vertebrates, including humans, domestic animals, poultry and wild game. Numerous Brachylaimoidea, particularly adults of Brachylaima and Leucochloridium, are difficult to identify to species. We provide and analyse sequences of two nuclear (18S rDNA, ITS2) and two mitochondrial (CO1, ND1) DNA loci of central European species of the Brachylaimoidea, namely Leucochloridium holostomum, Leucochloridium paradoxum, Leucochloridium perturbatum, Leucochloridium subtilis, Leucochloridium vogtianum, Urotocus rossitensis, Urogonimus macrostomus, Michajlovia migrata, Leucochloridiomorpha lutea, Brachylaima arcuatus, Brachylaima fuscata and Brachylaima mesostoma. We identified three clades in the genus Leucochloridium, which do not correspond to the previously suggested subgenera Neoleucochloridium, Papilloleucochloridium and Leucochloridium. We reject classification of Urotocus and Urogonimus in Leucochloridiinae, and, instead, re-establish the subfamilies Urotocinae and Urogoniminae. We synonymize the genus Renylaima with the genus Brachylaima. We reject M. migrata as a member of Leucochloridiinae sensu stricto or Brachylaimidae suggested by some previous authors. We found that the previously sequenced Glaphyrostomum sp. does not cluster with any hitherto sequenced Brachylaimidae. We also provide comparative measurements of the examined central European Brachylaimoidea, address the the specificity of their localization in the host and discuss their host-specific prevalence and intensity of infections based on the extensive dataset of birds examined in 1962-2015.

  15. AUTOMOTIVE DIESEL MAINTENANCE 2. UNIT VIII, AUTOMATIC TRANSMISSIONS--ALLISON TORQUMATIC SERIES 5960, 6060, AND 8860 (PART II).

    ERIC Educational Resources Information Center

    Human Engineering Inst., Cleveland, OH.

    THIS MODULE OF A 25-MODULE COURSE IS DESIGNED TO DEVELOP AN UNDERSTANDING OF THE OPERATION AND MAINTENANCE OF SPECIFIC MODELS OF AUTOMATIC TRANSMISSIONS USED ON DIESEL POWERED VEHICLES. TOPICS ARE (1) GENERAL DESCRIPTION, (2) OPTIONAL EQUIPMENT, (3) TRANSMISSION POWER FLOW (SPLITTER SECTION), (4) TRANSMISSION POWER FLOW (RANGE SECTION), (5)…

  16. AUTOMOTIVE DIESEL MAINTENANCE 2. UNIT VII, AUTOMATIC TRANSMISSIONS--ALLISON, TORQUMATIC SERIES 5960 AND 6060 (PART I).

    ERIC Educational Resources Information Center

    Human Engineering Inst., Cleveland, OH.

    THIS MODULE OF A 25-MODULE COURSE IS DESIGNED TO DEVELOP AN UNDERSTANDING OF THE OPERATION AND MAINTENANCE OF SPECIFIC MODELS OF AUTOMATIC TRANSMISSIONS USED ON DIESEL POWERED VEHICLES. TOPICS ARE (1) GENERAL SPECIFICATION DATA, (2) OPTIONS FOR VARIOUS APPLICATIONS, (3) ROAD TEST INSTRUCTIONS, (4) IDENTIFICATION AND SPECIFICATION DATA, (5) ALLISON…

  17. G&T adds versatile load management system

    SciTech Connect

    Nickel, J.R.; Baker, E.D.; Holt, J.W.; Chan, M.L.

    1995-04-01

    Wolverine`s load management system was designed in response to the need to reduce peak demand. The Energy Management System (EMS) prepares short term (seven day) load forecasts, based on a daily peak demand forecst, augmented by a similar day profile based on weather conditions. The software combines the similar day profile with the daily peak demand forecast to yield an hourly load forecast for an entire week. The software uses the accepted load forecast case in many application functions, including interchange scheduling, unit commitment, and transaction evaluation. In real time, the computer updates the accepted forecast hourly, based in actual changes in the weather and load. The load management program executes hourly. The program uses impact curves to calculate a load management strategy that reduces the load forecast below a desired load threshold.

  18. A Faraday Rotation Investigation to Probe the Shells of HII Regions with Associated Stellar Bubbles%A Costa, Allison H.; Spangler, Steven R.; Sink, Joseph R.

    NASA Astrophysics Data System (ADS)

    Costa, Allison H.; Spangler, Steven R.; Sink, Joseph R.

    2015-01-01

    We performed polarimetric observations with the Very Large Array of 11 extra-galactic radio sources with lines of sight through the shell of the Rosette Nebula (VLA project AS1110). This investigation supplements the study of the shell of the Rosette Nebula by Savage et al. (2013). We utilize two methods for calculating the rotation measure (RM) for the new lines of sight. The first is using the traditional method of least-squares fit to χ(λ2), and the second is using RM Synthesis (Brentjens & Bruyn 2005). We present a comparison of the two methods of obtaining RM values, and we find excellent agreement between the two methods. In addition, we discuss a plasma shell model, which is intended to reproduce the sign and magnitude of the observed RM due to the shell, with a new method of determining the model parameters, such as the inner and outer radii and the electron density, for specific lines of sight. This treatment of the model parameters allows us to extend our investigation to HII regions that do not have spherical symmetry, like the Heart Nebula/W4. We describe a new method of fitting a local shell model to specific lines of sight and then calculating the associated RM. By modeling the magnitude of the RM for specific lines of sight, we can better distinguish between a scenario where an observed excess in RM is due to an amplification of the magnetic field or one where it is exclusively due to an increase in the density of the shell. This research was supported at the University of Iowa by grant AST09-07911 and ATM09-56901 from the National Science Foundation.

  19. GT-MHR power conversion system: Design status and technical issues

    SciTech Connect

    Etzel, K.; Baccaglini, G.; Schwartz, A.; Hillman, S.; Mathis, D.

    1994-12-01

    The Modular Helium Reactor (MHR) builds on 30 years of international gas-cooled reactor experience utilizing the unique properties of helium gas coolant, graphite moderator and coated particle fuel. To efficiently utilize the high temperature potential of the MHR, an innovative power conversion system has been developed featuring an intercooled and recuperated gas turbine. The gas turbine replaces a conventional steam turbine and its many auxiliary components. The Power Conversion System converts the thermal energy of the helium directly into electrical energy utilizing a closed Brayton cycle. The Power Conversion System draws on even more years of experience than the MHR: the world`s first closed-cycle plant, fossil fired and utilizing air as working fluid, started operation in Switzerland in 1939. Shortly thereafter, in 1945, the coupling of a closed-cycle plant to a nuclear heat generation system was conceived. Directly coupling the closed-cycle gas turbine concept to a modern, passively safe nuclear reactor opens a new chapter in power generation technology and brings with it various design challenges. Some of these challenges are associated with the direct coupling of the Power Conversion System to a nuclear reactor. Since the primary coolant is also the working fluid, the Power Conversion System has to be designed for reactor radionuclide plateout. As a result, issues like component maintainability and replaceability, and fission product effects on materials must be addressed. Other issues concern the integration of the Power Conversion System components into a single vessel. These issues include the selection of key technologies for the power conversion components such as submerged generator, magnetic bearings, seals, compact heat exchangers, and the overall system layout.

  20. Use of Conversion Adaptors to Clone Antigen Genes in Lambda gt11

    DTIC Science & Technology

    1987-01-01

    gradients of 19, 30, and 50%. with 4 units ofT 4 DNA ligase for 60 min at Chromosomal DNA was prepared by dode- 16°C. Because the adaptor-insert...0.75 M and 6.5%. respectively. After chill- Biotec. Madison. WI) and 0.5 unit of T4 ing on ice for I h. the mixture was centri- DNA ligase , in 5ul of

  1. GT1_abenz_1: Completing the OH ladder for HH 46

    NASA Astrophysics Data System (ADS)

    Benz, A.

    2010-03-01

    First results from PACS observations towards the low-mass protostar HH 46 show surprisingly bright OH lines. The hydroxyl radical OH plays important roles in the water and oxygen chemistry of star-forming regions and their cooling. Furthermore, the hydroxyl-to-water line ratios are interesting tracers for ionizing radiation. We propose a nearly complete observation of the OH ladder in low-mass star formation for the first time. Four OH transitions in the class I object HH 46 were detected by PACS. We propose complementary observations towards the source in PACS line spectroscopy mode at 53, 56, 65, 71, 96, 115 and 135 micron. We gain insight in the origin and formation of OH from the PACS spatial information. The completeness of the OH ladder allows a reliable determination of the OH abundance and thus constrains water chemistry and cooling contribution more precisely. In addition, we propose HIFI observations of the OH transition at 163.4 micron to resolve the three hyperfine components for the first time. This will allow to determine optical depths of OH and test the hypothesis of asymmetries between the two closely spaced triplets, as the second triplet will be observed within the HIFI priority science program. Note: This proposal is submitted under the Swiss part of the HIFI guaranteed time program; HIFI PI: Frank Helmich, HIFI Swiss Lead CoI: Arnold O. Benz

  2. GT1_vgeers_1: Tracing Remnant Gas in Planet Forming Debris Disk Systems

    NASA Astrophysics Data System (ADS)

    Geers, V.

    2010-03-01

    Recent studies of gas emission lines with Spitzer and sub-millimeter telescopes have shown that 10-100 Myr old stars with debris disks have too little gas left to form Jupiter like gas giant planets. Whether enough gas remains in these systems to form ice giant planets is still unanswered. The [OI] emission line at 63 micron is one of the most sensitive tracers of gas mass in the ice-giant region of 10-50 AU in disks, and Herschel PACS is therefore uniquely suited to trace the remnant gas in planet-forming disks. We propose to obtain PACS line spectroscopy of [OI] (63 micron) for two nearby young stars, HR 8799 and HD 15115, which are two systems with detected giant planets or signs of planet formation, while still harbouring prominent debris disks that could be in the process of forming ice giants such as Neptune and Uranus. The proposed observations will probe down to gas masses of 0.01 Earth masses, and allow us to constrain prospects for ice giant formation, measure gas-to-dust ratios in evolved disks to compare with planet formation / disk evolution models, and put constraints on whether the dust dynamics in these systems is driven by the remnant gas or by the radiation. Note: this proposal is submitted under the Swiss part of the HIFI Guaranteed Time program; HIFI PI: Frank Helmich, HIFI Swiss Lead CoI: Arnold Benz.

  3. GT1_cdedes_1: Heating and cooling mechanics in massive star formation

    NASA Astrophysics Data System (ADS)

    Dedes, C.

    2010-03-01

    Massive stars are important constituents of the interstellar medium (ISM) in our Galaxy and beyond. Their strong feedback processes influence the dynamics, energetics and chemistry of the surrounding interstellar medium both locally and on large scales. An important question to be answered is the one of cooling and heating mechanisms in regions of massive star formation. In the vicinity of massive stars, heating is provided mostly by far-UV (FUV) and infra-red radiation. Cooling is mostly provided by emission in the fine structure lines of CII. There are however other atomic and molecular lines such as OI, CO, OH and H_2O which can become significant coolants in the dense, embedded regions of massive star formation. This early phase when the forming massive star is still deeply embedded in its natal envelope, yet already interacting with, and potentially destroying, its environment through copious amounts of UV radiation, massive outflows and ultra compact HII (UCHII) regions, is an important phase in the star formation process. To understand the heating and cooling balance in this phase, one has to consider the contributions of various radiative and dynamical processes such as the FUV radiation from the young star itself, shocks created by strong stellar winds and the photon dominated regions (PDRs) where the radiation impinges on the molecular material. The tracers of these processes can be observed in the far-infrared, a wavelength range that is now accessible at unprecedented high spectral and spatial resolution with the Herschel Space Observatory. We propose to observe the aformentioned tracers of cooling and heating in the massive star forming region IRAS 12326-6245 to obtain a complete picture of the different processes, the regions they originate from and how they interact. This proposal is for time granted to the HIFI hardware team (PI: Frank Helmich) and to be accounted as part of the Swiss guaranteed time (Lead-Co-I: Arnold O. Benz).

  4. NO{sub x}-abatement potential of lean-premixed GT combustors

    SciTech Connect

    Sattelmayer, T.; Polifke, W.; Winkler, D.; Doebbeling, K.

    1998-01-01

    The influence of the structure of perfectly premixed flames on NO{sub x} formation is investigated theoretically. Since a network of reaction kinetics modules and model flames is used for this purpose, the results obtained are independent of specific burner geometries. Calculations are presented for a mixture temperature of 630 K, an adiabatic flame temperature of 1840 K, and 1 and 15 bars combustor pressure. In particular, the following effects are studied separately from each other: molecular diffusion of temperature and species, flame strain, local quench in highly strained flames and subsequent reignition, turbulent diffusion (no preferential diffusion), and small scale mixing (stirring) in the flame front. Either no relevant influence or an increase in NO{sub x} burners is to avoid excessive turbulent stirring in the flame front. Turbulent flames that exhibit locally and instantaneously near laminar structures (flamelets) appear to be optimal. Using the same methodology, the scope of the investigation is extended to lean-lean staging, since a higher NO{sub x}-abatement potential can be expected in principle. As long as the chemical reactions of the second stage take place in the boundary between the fresh mixture of the second stage and the combustion products from upstream, no advantage can be expected from lean-lean staging. Only if the preliminary burner exhibits much poorer mixing than the second stage can lean-lean staging be beneficial. In contrast, if full mixing between the two stages prior to afterburning can be achieved (lean-mix-lean technique), the combustor outlet temperature can in principle be increased somewhat without NO penalty.

  5. Characterisation of reservoir fluid conditions at geothermal well Gross Buchholz Gt1

    NASA Astrophysics Data System (ADS)

    Hesshaus, A.; Kringel, R.; Houben, G.; Tischner, T.

    2012-04-01

    In 2009 an approximately 4 km deep geothermal well was drilled in Hannover, Germany. The intention of the project is to provide thermal energy for the Geocentre in Hannover (office complex with a heat demand equivalent to 2 MW) It is intended to produce energy out of tight sediments of the northern German basin from a single well. As the target formation (Bunter Sandstone) has only a marginal porosity, an underground heat exchanger had to be created by a hydraulic frac operation. The frac was generated by injecting 20.000 m3 of fresh water into the well. After six month of enclosure the water was recovered from the formation. While producing the fluid was sampled several times to analyse dissolved mineral content, gases and possibly particles. The geochemistry of the reservoir is of particular interest for the use and maintenance of the future geothermal system. Only a good understanding of the underground conditions allows a successful operation of the heat supply system. In this study, the chemical composition of the water and the nature of the gas phase are presented. In addition, the chemical and mineralogical composition of scales, which developed during the test, is addressed. The radioactivity of water, gas, and scale were also studied for occupational safety reasons. The obtained water at the well head became oversaturated with respect to NaCl at surface conditions. Therefore massive scaling occurred on the surface equipment. After a few days of production the test had to be stopped. The water is a very hard Na-Ca-Cl-brine (total dissolved solids (TDS) 340 g/L). Concentrations at the bottom of the well were probably even higher as cooling-induced precipitation occurs during passage to the surface. If the formation water were in equilibrium with halite, the TDS should be ca. 370 g/L at 160 °C. The pH value ranges between 5 and 6 at surface conditions after partial degassing. The redox conditions are anoxic with high concentrations of Fe(II), Mn(II) and NH4+. The gas phase mostly consists of nitrogen (90 Vol%) and carbon dioxide (10 Vol%). A normal geogenic content of 222Rn in the gas phase was detected, corresponding to relatively low radioactivity (50 Bq/L). In both, water and scale, concentrations of radioactive compounds were well below threshold values. In order to characterise mixing and dissolution processes the stable isotopes of the injected water and the reproduced water were also analysed. The recovered water reflects the signature of deep formation water. This result along with the high salt content shows a high percentage of formation fluid. Its composition is most likely dominated by a mixture of reservoir fluid and dissolution of some cementation minerals, like halite and anhydrite. It remains to be shown, if oversaturated water occurs at any production test or if a state of subsaturation can be achieved by repeated injection of fresh water. More tests are needed to answer this question.

  6. An Array of Services: Least Restrictive Environments for G/T Kids

    ERIC Educational Resources Information Center

    Lurz, Priscilla Ramirez

    2007-01-01

    While people have learned much from special education about using an array of services, from the least restrictive to the most restrictive learning environments, public schools still base many instructional decisions on a child's chronological age. Are the child's interests best served by being grouped all day solely by the number of years lived?…

  7. Rapid Mapping of AR Coating Thickness on Si Solar Cells Using GT-FabScan 6000

    SciTech Connect

    Sopori, B.; Amieva, J.; Butterfield, B.; Li, C.

    2005-02-01

    A new technique for rapid mapping of the thickness of an antireflection (AR) coating on a solar cell is described. A filtered, reflectance (intensity) image of the AR-coated wafer is generated by a CCD camera mounted on a GTFabScan. This image is converted into a thickness image using a transformation relating local AR thickness to the local intensity in the image plane. The thickness map is generated in <100 ms.

  8. Evaluation of fuel injection configurations to control carbon and soot formation in small GT combustors

    NASA Technical Reports Server (NTRS)

    Rosfjord, T. J.; Briehl, D.

    1982-01-01

    An experimental program to investigate hardware configurations which attempt to minimize carbon formation and soot production without sacrificing performance in small gas turbine combustors has been conducted at the United Technologies Research Center. Four fuel injectors, embodying either airblast atomization, pressure atomization, or fuel vaporization techniques, were combined with nozzle air swirlers and injector sheaths, and evaluated at test conditions which included and extended beyond standard small gas turbine combustor operation. Extensive testing was accomplished with configurations embodying either a spill return or a T-vaporizer injector. Minimal carbon deposits were observed on the spill return nozzle for tests using either Jet A or ERBS test fuel. A more extensive film of soft carbon was observed on the vaporizer after operation at standard engine conditions, with large carbonaceous growths forming on the device during off-design operation at low combustor inlet temperature. Test results indicated that smoke emission levels depended on the combustor fluid mechanics (especially the mixing rates near the injector), the atomization quality of the injector and the fuel hydrogen content.

  9. A novel role for Escherichia coli endonuclease VIII in prevention of spontaneous G-->T transversions.

    PubMed

    Blaisdell, J O; Hatahet, Z; Wallace, S S

    1999-10-01

    In the bacterium Escherichia coli, oxidized pyrimidines are removed by two DNA glycosylases, endonuclease III and endonuclease VIII (endo VIII), encoded by the nth and nei genes, respectively. Double mutants lacking both of these activities exhibit a high spontaneous mutation frequency, and here we show that all of the mutations observed in the double mutants were G:C-->A:T transitions; no thymine mutations were found. These findings are in agreement with the preponderance of C-->T transitions in the oxidative and spontaneous mutational databases. The major oxidized purine lesion in DNA, 7,8-dihydro-8-oxoguanine (8-oxoG), is processed by two DNA glycosylases, formamidopyrimidine DNA glycosylase (Fpg), which removes 8-oxoG opposite C, and MutY DNA glycosylase, which removes misincorporated A opposite 8-oxoG. The high spontaneous mutation frequency previously observed in fpg mutY double mutants was significantly enhanced by the addition of the nei mutation, suggesting an overlap in the substrate specificities between endo VIII and Fpg/MutY. When the mutational specificity was examined, all of the mutations observed were G:C-->T:A transversions, indicating that in the absence of Fpg and MutY, endo VIII serves as a backup activity to remove 8-oxoG. This was confirmed by showing that, indeed, endo VIII can recognize 8-oxoG in vitro.

  10. GT1_mbaes_1: HERschel Observations of Edge-on Spirals (HEROES)

    NASA Astrophysics Data System (ADS)

    Baes, M.

    2010-03-01

    We propose to use PACS and SPIRE to map the dust distribution in a sample of seven large edge-on spiral galaxies with regular dust lanes. We will look for the presence of cold dust at large galactocentric radii and investigate the link between dust, gas and metallicity as a function of radius. We will also constrain the vertical distribution of the dust and particularly look for dust emission at large heights above the plane of the galaxies. We will compare the observed Herschel maps with simulated maps resulting from detailed radiative transfer models based on optical and near-infrared images. This will enable us to investigate whether we can confirm the existence of a dust energy balance problem suggested by previous observations (the dust seen in absorption in optical maps underestimates the dust seen in emission) and investigate possible ways to alleviate this potential problem.

  11. GT2_rguesten_5: Spectral mapping of the closest galactic nucleus

    NASA Astrophysics Data System (ADS)

    Guesten, R.

    2011-05-01

    Due to its proximity, our Galactic Center provides a unique opportunity to study the physical processes that occur within (extra)galactic nuclei with unprecedented sensitivity and spatial resolution. Herschel provides the only opportunity to probe the detailed physical conditions of the circumnuclear neutral material and the feedback processes with the central energy source(s) in our own Galaxy, and by extension in other galactic nuclei. We propose to acquire wide range (51 to 670 micron) spectral maps towards the atomic and molecular gas surrounding SgrA. Combining data from the PACS and SPIRE instruments, we will establish a complete inventory of the FIR spectrum accessible to Herschel across the central 5 arcmin (12 pc) of the Galaxy. Our immediate goal is to characterize the physical and chemical conditions of the gas in both the central (ionized) cavity and in the surrounding neutral gas (often referred to as circumnuclear disk, or CND) that is presumably the reservoir of material that can flow to the central black hole. Our supporting ground-based and first targeted HIFI observations show a high-excitation state of the molecular gas in the CND, with a strong radial excitation gradient across the disk and a very sharp inner transition zone to the central cavity. These early data resemble the hard XDR-type CO line SED observed towards e.g. Mrk231.Herschel data cover lines from energy levels needed to constrain the conditions within, and energetics of the CND. We will use state-of-the-art XDR/PDR models that include multiple heating processes (a.o., shock, dissipation of turbulence) to interpret the data. The data product will be one of Herschel's legacies in the detailed study of galactic nuclei. This data set, with high sensitivity, high spatial resolution, and broad wavelength coverage, will be an essential resource for many studies of the Galactic Center. No comparable study of this region will be possible for many years to come.

  12. Structural and Biochemical Studies of the 5 - gt 3 Exoribonuclease Xrn1

    SciTech Connect

    J Chang; S Xiang; K Xiang; J Manley; L Tong

    2011-12-31

    The 5' {yields} 3' exoribonucleases (XRNs) have important functions in transcription, RNA metabolism and RNA interference. The structure of Rat1 (also known as Xrn2) showed that the two highly conserved regions of XRNs form a single, large domain that defines the active site of the enzyme. Xrn1 has a 510-residue segment after the conserved regions that is required for activity but is absent from Rat1/Xrn2. Here we report the crystal structures of Kluyveromyces lactis Xrn1 (residues 1-1,245, E178Q mutant), alone and in complex with a Mn{sup 2+} ion in the active site. The 510-residue segment contains four domains (D1-D4), located far from the active site. Our mutagenesis and biochemical studies show that their functional importance results from their ability to stabilize the conformation of the N-terminal segment of Xrn1. These domains might also constitute a platform that interacts with protein partners of Xrn1.

  13. Retraction: 'Number needed to treat with 4-factor prothrombin complex concentrate for urgent warfarin reversal' by Andrew Chua, Vishal Patel, Allison Perrin, Lee Stern, Jenifer Ehreth, Laurel Omert, Christopher Hood, Julie Farley, Michael McGlynn and Liping Huang.

    PubMed

    2017-04-01

    The above abstract from the THSNA 2016 Summit Abstract Proceedings, first published online in the American Journal of Hematology on 20 July 2016 in Wiley OnlineLibrary (www.onlinelibrary.wiley.com), and in Volume 91, Issue 9, p. E427, has been retracted by agreement between the authors, the journal Editor-in-Chief, Carlo Brugnara, and Wiley Periodicals, Inc. The retraction has been agreed due to concerns from the submitting authors that the abstract was inadvertently submitted prior to receiving approval from all authors and proper review of data analytics, thereby rendering it incomplete.

  14. MODELING MONOMETHYLMERCURY AND TRIBUTYLTIN SPECIATION WITH EPA'S GEOCHEMICAL SPECIATION MODEL MINTEQA2

    EPA Science Inventory

    Given the complexity of the various, simultaneous (and competing) equilibrium reactions governing the speciation of ionic species in aquatic systems, EPA has developed and distributed the geochemical speciation model MINTEQA2 (Brown and Allison, 1987, Allison et al., 1991; Hydrog...

  15. The Arizona Geological Survey | Home

    Science.gov Websites

    Aug 18, 2016 | AGI's EARTH Magazine releases 2012 interview with AZGS's Lee Allison. Aug 17, 2016 | Dr. M. Lee Allison (1948-2016), State Geologist and Director of the Arizona ...

  16. Making Optic Flow Robust to Dynamic Lighting Conditions for Real-Time Operation

    DTIC Science & Technology

    2016-03-17

    Operation by Allison Mathis, William Nothwang, Daniel Donavanik, Joseph Conroy, Jared Shamwell, and Ryan Robinson...by Allison Mathis, William Nothwang, Daniel Donavanik, Joseph Conroy, Jared Shamwell, and Ryan Robinson Sensors and Electron Devices Directorate, ARL...NUMBER 6. AUTHOR(S) Allison Mathis, William Nothwang, Daniel Donavanik, Joseph Conroy, Jared Shamwell, and Ryan Robinson 5d. PROJECT NUMBER

  17. Effects of surface condition on the corrosion of candidate structural materials in a simulated HTGR-GT environment

    SciTech Connect

    Thompson, L.D.

    1980-02-01

    A simulated high-temperature gas-cooled reactor (HTGR) helium environment was used to study the effects of surface finish conditions on the subsequent elevated-temperature corrosion behavior of key candidate structural materials. The environment contained helium with 500 ..mu..atm H/sub 2//50 ..mu..atm CO/50 ..mu..atm CH/sub 4//<0.5 ..mu..atm H/sub 2/O at 900/sup 0/C with total test exposure durations of 3000 hours. Specimens with lapped, grit-blasted, pickled, and preoxidized surface conditions were studied. Materials tested included two cast superalloys, IN 100 and IN 713LC; one centrifugally cast high-temperature alloy, HK 40 one oxice-dispersion-strengthened alloy, Inconel MA 754; and three wrought high-temperature alloys, Hastelloy Alloy X, Inconel Alloy 617, and Alloy 800H.

  18. 75 FR 78596 - Airworthiness Directives; Hawker Beechcraft Corporation Models B200, B200GT, B300, and B300C...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-16

    ...-hours x $85 $100 $1,375 $123,750 pneumatic supply tube for the per hour = $1,275. tail de-ice system.... Action Labor Cost Parts cost product operators Fabricate placard (retained action 1 work-hour x $85 per... Bulletin SB 30- bulkhead at FS 347.750 with December 20, 2010 3889, Rev 1, dated Hytrel tubing part...

  19. GT-MHR COMMERCIALIZATION STUDY TECHNICAL PROGRESS AND COST MANAGEMENT REPORT FOR THE PERIOD NOVEMBER 1 THROUGH NOVEMBER 30, 2003

    SciTech Connect

    SHENOY,AS

    2003-12-01

    OAK-B135 A copy of the original Memorandum of Understanding (MOU) between the US and the US for the HFR-EU2 (formally MHR-1) fuel irradiation test was sent to DOE-HQ. The startup of the HFR-EU2 test will likely be delayed until September 2004 depending on completion and check out of the fission gas sweep loop. The initial core nuclear design study using advanced fuel was completed for a Very High Temperature Reactor (VHTR). This study assessed the nuclear effects of replacing the silicon carbide layer of the TRISO particle with a zirconium carbide layer (TRIZO particle). The study determined that zirconium behaves like a non-burnable poison and to achieve fuel cycle lengths equivalent to those with TRISO fuel, the fixed burnable poison would have to be adjusted. The core nuclear design study is documented in report General Atomics report number PC-000514. A test plan was completed that describes a series of screening tests to provide the technical basis for selecting and qualifying an advanced coated-particle fuel for the VHTR. The test plan is documented in General Atomics report number PC-000510.

  20. Comparison of cage and mast with the alcohol markers CDT, gamma-GT, ALAT, ASAT and MCV.

    PubMed

    Wetterling, T; Kanitz, R D; Rumpf, H J; Hapke, U; Fischer, D

    1998-01-01

    Many alcoholics deny abuse. To screen greater samples for alcohol dependence, short questionnaires, e.g. the CAGE or MAST are often applied. Frequently laboratory parameters [i.e. 'alcohol markers', such as carbohydrate-deficient transferrin (CDT), gamma-glutamyl transferase or mean corpuscular volume of erythrocytes] are used to support the diagnosis of long-standing heavy alcohol consumption. In this study, the self-ratings (CAGE and MAST) were compared with the above laboratory parameters in an unselected sample of 204 patients admitted to a general hospital. The sensitivities, specificities, and positive (PPV) as well as negative predictive values of the CAGE, the MAST, and the alcohol markers were calculated along with the reported alcohol consumption or the ICD-10 diagnosis as standard. According to recent harmful alcohol consumption levels (women >225 g/week: men >350 g/week), the sensitivities and the PPVs were rather low in all tests (sensitivity <60%; PPV <50%). With the ICD-10 diagnosis as standard, the CAGE and MAST showed a rather high specificity (>95%) and PPV (about 90%). CDT revealed the best PPV of all alcohol markers (60%). However, the sensitivity of the CAGE, MAST, and the alcohol markers for the ICD-10 diagnosis was rather poor (<60%). This low sensitivity impedes the usefulness of these questionnaires and alcohol markers as screening tests for alcoholism in general hospitals.

  1. Michel accretion of a polytropic fluid with adiabatic index \\gamma \\gt 5/3: global flows versus homoclinic orbits

    NASA Astrophysics Data System (ADS)

    Chaverra, Eliana; Mach, Patryk; Sarbach, Olivier

    2016-05-01

    We analyze the properties of a polytropic fluid that is radially accreted into a Schwarzschild black hole. The case where the adiabatic index γ lies in the range of 1\\lt γ ≤slant 5/3 has been treated in previous work. In this article, we analyze the complementary range of 5/3\\lt γ ≤slant 2. To this purpose, the problem is cast into an appropriate Hamiltonian dynamical system, whose phase flow is analyzed. While, for 1\\lt γ ≤slant 5/3, the solutions are always characterized by the presence of a unique critical saddle point, we show that, when 5/3\\lt γ ≤slant 2, an additional critical point might appear, which is a center point. For the parametrization used in this paper, we prove that, whenever this additional critical point appears, there is a homoclinic orbit. Solutions corresponding to homoclinic orbits differ from standard transonic solutions with vanishing asymptotic velocities in two aspects: they are local (i.e., they cannot be continued to arbitrarily large radii); the dependence of the density or the value of the velocity on the radius is not monotonic.

  2. Effect of Chlorotriazine Pesticides on Gonadotrophin Releasing Hormone in the Neuronal GT1-7 Cell Line and Hypothalamic Explants

    EPA Science Inventory

    Gonadotrophin releasing hormone (GnRH) stimulates the release of pituitary luteinizing hormone (LH) and follicle stimulating hormone. These pituitary hormones are necessary for normal reproductive function in both males and females. It is well recognized that disruption of nor...

  3. [Hereditary fructose intolerance (HFI) as cause of isolated gamma GT rise in a 5-year old boy with hepatomegaly].

    PubMed

    Wössmann, W; Wiemann, J; Körber, F; Gortner, L

    2000-01-01

    The diagnosis of HFI is easily missed during childhood. It should be suspected in children presenting with hepatomegaly and an isolated increase in GGT. A carefully taken nutritional history forms the basis of the diagnosis of HFI which can be confirmed by molecular analysis with a sensitivity of > 95%. I.v. fructose tolerance tests and liver biopsies often can be omitted.

  4. First Detection of &gt100 MeV Gamma Rays Associated with a Behind-the-limb Solar Flare

    NASA Astrophysics Data System (ADS)

    Pesce-Rollins, M.; Omodei, N.; Petrosian, V.; Liu, Wei; Rubio da Costa, Fatima; Allafort, A.; Chen, Qingrong

    2015-06-01

    We report the first detection of >100 MeV gamma-rays associated with a behind-the-limb solar flare, which presents a unique opportunity to probe the underlying physics of high-energy flare emission and particle acceleration. On 2013 October 11 a GOES M1.5 class solar flare occurred ∼9.°9 behind the solar limb as observed by STEREO-B. RHESSI observed hard X-ray (HXR) emission above the limb, most likely from the flare loop-top, as the footpoints were occulted. Surprisingly, the Fermi Large Area Telescope (LAT) detected >100 MeV gamma-rays for ∼30 minutes with energies up to 3 GeV. The LAT emission centroid is consistent with the RHESSI HXR source, but its uncertainty does not constrain the source to be located there. The gamma-ray spectra can be adequately described by bremsstrahlung radiation from relativistic electrons having a relatively hard power-law (PL) spectrum with a high-energy exponential cutoff, or by the decay of pions produced by accelerated protons and ions with an isotropic pitch-angle distribution and a PL spectrum with a number index of ∼3.8. We show that high optical depths rule out the gamma-rays originating from the flare site and a high-corona trap model requires very unusual conditions, so a scenario in which some of the particles accelerated by the CME shock travel to the visible side of the Sun to produce the observed gamma-rays may be at work.

  5. TPH2 G/T Polymorphism Is Associated with Hyperphagia, IQ, and Internalizing Problems in Prader-Willi Syndrome

    ERIC Educational Resources Information Center

    Dykens, Elisabeth M.; Roof, Elizabeth; Bittel, Douglas; Butler, Merlin G.

    2011-01-01

    Background: Prader-Willi syndrome (PWS) is a genetic, neurodevelopmental disorder characterized by intellectual disabilities, growth hormone dysregulation, hyperphagia, increased risks of morbid obesity, compulsive behaviors, and irritability. As aberrant serotonergic functioning is strongly implicated in PWS, we examined associations between the…

  6. 78 FR 20386 - Notice of Receipt of Petition for Decision That Nonconforming 2012 Porsche GT3RS Passenger Cars...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-04

    ....-model interior trunk release components. The petitioner states that the bumpers and bumper support structure are identical to that of the U.S. certified model. However, the bumper shocks must be...

  7. Prediction of a Newbuilding Proce of the Bulk Carriers based on Gross Tonnage GT and Main Engine Power

    NASA Astrophysics Data System (ADS)

    Cepowska, Żaneta; Cepowski, Tomasz

    2017-03-01

    The paper presents mathematical relationships that allow us to forecast the newbuilding price of new bulk carriers, based on data concerning vessels built in 2005-2015. The presented approximations allow us to estimate the price based on a gross tonnage capacity and a main engine power The approximations were developed using linear regression and the theory of artificial neural networks. The presented relations have practical application for estimation of bulk carrier newbuilding price needed in preliminary parametric design of the ship. It follows from the above that the use of artificial neural networks to predict the price of a bulk carrier brings more accurate solutions than linear regression.

  8. Assessment of CO2 Sequestration and ECBM Potential of U.S. Coalbeds

    SciTech Connect

    Scott R. Reeves

    2003-03-31

    In October, 2000, the U.S. Department of Energy, through contractor Advanced Resources International, launched a multi-year government-industry R&D collaboration called the Coal-Seq project. The Coal-Seq project is investigating the feasibility of CO{sub 2} sequestration in deep, unmineable coalseams, by performing detailed reservoir studies of two enhanced coalbed methane recovery (ECBM) field projects in the San Juan basin. The two sites are the Allison Unit, operated by Burlington Resources, and into which CO{sub 2} is being injected, and the Tiffany Unit, operating by BP America, into which N{sub 2} is being injected (the interest in understanding the N{sub 2}-ECBM process has important implications for CO{sub 2} sequestration via flue-gas injection). The purposes of the field studies are to understand the reservoir mechanisms of CO{sub 2} and N{sub 2} injection into coalseams, demonstrate the practical effectiveness of the ECBM and sequestration processes, an engineering capability to simulate them, and to evaluate sequestration economics. In support of these efforts, laboratory and theoretical studies are also being performed to understand and model multi-component isotherm behavior, and coal permeability changes due to swelling with CO{sub 2} injection. This report describes the results of an important component of the overall project, applying the findings from the San Juan Basin to a national scale to develop a preliminary assessment of the CO{sub 2} sequestration and ECBM recovery potential of U.S. coalbeds. Importantly, this assessment improves upon previous investigations by (1) including a more comprehensive list of U.S. coal basins, (2) adopting technical rationale for setting upper-bound limits on the results, and (3) incorporating new information on CO{sub 2}/CH{sub 4} replacement ratios as a function of coal rank. Based on the results of the assessment, the following conclusions have been drawn: (1) The CO{sub 2} sequestration capacity of U

  9. Effects of Using Subtest Standard Scores in Calculating AFQT (Armed Forces Qualification Test) Percentile Scores

    DTIC Science & Technology

    1986-05-01

    STDMC (STDEI (STDVE LT LT LT LT LT LT LT LT LT LT LT GT GT GT GT GT GT GT GT GT GT GT 20) 20) 20) 20) 20) 20) 20) 20) 20) 20) 20) 80 ) 80 ) 80 ) 80 ) 80 ) 80 ) 80 ... 80 ) 80 ) 80 ) 80 ) STDGS=20 STDAR=20 STDWK=20 STDPC=20 STDNO=20 STDCS=20 STDAS=20 STDMK=20 STDMC=20 STDEI=20 STDVE=20 STDGS =80 STDAR=80 STDWK=80

  10. Support and power plant documentation for the gas turbine powered bus demonstration program

    NASA Technical Reports Server (NTRS)

    Nigro, D. N.; Stewart, R. G.; Apple, S. A.

    1982-01-01

    The operational experience obtained for the GT404-4 gas turbine engines in the intercity and intracity Bus Demonstration Programs is described for the period January 1980 through September 1981. Support for the engines and automatic transmissions involved in this program provided engineering and field service, spare parts and tools, training, and factory overhauls. the Greyhound (intercity) coaches accumulated 183,054 mi (294,595 km) and 5154 hr of total operation. The Baltimore Transit (intracity) coaches accumulated 40,567 mi (65,285 km) and 1840 hr of total operation. In service, the turbine powered Greyhound and Transit coaches achieved approximately 25% and 40% lower fuel mileage, respectively, than did the production diesel powered coaches. The gas turbine engine will require the advanced ceramic development currently being sponsored by the DOE and NASA to achieve fuel economy equivalent not only to that of today's diesel engines but also to the projected fuel economy of the advanced diesel engines of the 1990s. Sufficient experience was not achieved with the coaches prior to the start of service to identify and eliminate many of the problems associated with the startup of new equipment. Because of these problems, the mean miles between incident were unacceptably low. The future gas turbine system should be developed sufficiently to establish satisfactory durability prior to evaluation in revenue service. Commercialization of the gas turbine bus engine remains a viable goal for the future.

  11. Cognitive Biases and Structural Failures in United States Foreign Policy: Explaining Decision-Making Dissonance in Phase IV Policy and Plans for Iraq

    DTIC Science & Technology

    2007-12-01

    Present, and Future, Third ed. (New Jersey: Prentice Hall, 1997), 235. See also Allison , Essence of Decision, 13. 16 Hans J. Morgenthau, "A...value maximizing.18 Graham Allison posits that states select courses of action where the likelihood of achieving “goals and objectives” for a given...Stanford, CA: Stanford University Press, 1999), 4. 18 Graham T. Allison , "Conceptual Models and the Cuban Missile Crisis" In American Foreign Policy

  12. Identification of Small Molecules against Botulinum Neurotoxin B Binding to Neuronal Cells at Ganglioside GT1b Binding Site with Low to Moderate Affinity

    DTIC Science & Technology

    2014-10-01

    recorded using a SpectraMax Plus plate reader with SoftMax ProVer 5.4 software (Molecular Devices, CA). Cell line: Human neuroblastoma cell line SH... neuroblastoma cell line SH-SY5Y (Table 2). Although most of the compounds were found to show some toxicity, three compounds were found to be relatively...50% are shown in red bold. Table 2 Compound toxicity for human neuroblastoma SH-SY5Y cell line. Sigmoidal Dose Response analysis except for

  13. What is a microsatellite: a computational and experimental definition based upon repeat mutational behavior at A/T and GT/AC repeats.

    PubMed

    Kelkar, Yogeshwar D; Strubczewski, Noelle; Hile, Suzanne E; Chiaromonte, Francesca; Eckert, Kristin A; Makova, Kateryna D

    2010-01-01

    Microsatellites are abundant in eukaryotic genomes and have high rates of strand slippage-induced repeat number alterations. They are popular genetic markers, and their mutations are associated with numerous neurological diseases. However, the minimal number of repeats required to constitute a microsatellite has been debated, and a definition of a microsatellite that considers its mutational behavior has been lacking. To define a microsatellite, we investigated slippage dynamics for a range of repeat sizes, utilizing two approaches. Computationally, we assessed length polymorphism at repeat loci in ten ENCODE regions resequenced in four human populations, assuming that the occurrence of polymorphism reflects strand slippage rates. Experimentally, we determined the in vitro DNA polymerase-mediated strand slippage error rates as a function of repeat number. In both approaches, we compared strand slippage rates at tandem repeats with the background slippage rates. We observed two distinct modes of mutational behavior. At small repeat numbers, slippage rates were low and indistinguishable from background measurements. A marked transition in mutability was observed as the repeat array lengthened, such that slippage rates at large repeat numbers were significantly higher than the background rates. For both mononucleotide and dinucleotide microsatellites studied, the transition length corresponded to a similar number of nucleotides (approximately 10). Thus, microsatellite threshold is determined not by the presence/absence of strand slippage at repeats but by an abrupt alteration in slippage rates relative to background. These findings have implications for understanding microsatellite mutagenesis, standardization of genome-wide microsatellite analyses, and predicting polymorphism levels of individual microsatellite loci.

  14. Nonsyndromic X-linked intellectual deficiency in three brothers with a novel MED12 missense mutation [c.5922G>T (p.Glu1974His)

    PubMed Central

    Bouazzi, Habib; Lesca, Gaetan; Trujillo, Carlos; Alwasiyah, Mohammad Khalid; Munnich, Arnold

    2015-01-01

    Key Clinical Message X-linked intellectual deficiency (XLID) is a large group of genetic disorders. MED12 gene causes syndromic and nonsyndromic forms of XLID. Only seven pathological mutations have been identified in this gene. Here, we report a novel mutation segregating with XLID phenotype. This mutation could be in favor of genotype–phenotype correlations. PMID:26273451

  15. Solar Technology Validation Project - Tri-State G&T: Cooperative Research and Development Final Report, CRADA Number CRD-09-367-12

    SciTech Connect

    Wilcox, S.

    2013-08-01

    Under this Agreement, NREL will work with Participant to improve concentrating solar power system performance characterizations. This work includes, but is not limited to, research and development of methods for acquiring renewable resource characterization information using site-specific measurements of solar radiation and meteorological conditions; collecting system performance data; and developing tools for improving the design, installation, operation, and maintenance of solar energy conversion systems. This work will be conducted at NREL and Participant facilities.

  16. Down-Regulation of PoGT47C Expression in Poplar Results in a Reduced Glucuronoxylan Content and an Increased Wood Digestibility by Cellulase

    EPA Science Inventory

    Xylan is the second most abundant polysaccharide in dicot wood. Unraveling the biosynthetic pathway of xylan is important not only for our understanding of the process of wood formation but also for our rational engineering of wood for biofuel production. Although several glycosy...

  17. Optimized Seizure Detection Algorithm: A Fast Approach for Onset of Epileptic in EEG Signals Using GT Discriminant Analysis and K-NN Classifier

    PubMed Central

    Rezaee, Kh.; Azizi, E.; Haddadnia, J.

    2016-01-01

    Background Epilepsy is a severe disorder of the central nervous system that predisposes the person to recurrent seizures. Fifty million people worldwide suffer from epilepsy; after Alzheimer’s and stroke, it is the third widespread nervous disorder. Objective In this paper, an algorithm to detect the onset of epileptic seizures based on the analysis of brain electrical signals (EEG) has been proposed. 844 hours of EEG were recorded form 23 pediatric patients consecutively with 163 occurrences of seizures. Signals had been collected from Children’s Hospital Boston with a sampling frequency of 256 Hz through 18 channels in order to assess epilepsy surgery. By selecting effective features from seizure and non-seizure signals of each individual and putting them into two categories, the proposed algorithm detects the onset of seizures quickly and with high sensitivity. Method In this algorithm, L-sec epochs of signals are displayed in form of a third-order tensor in spatial, spectral and temporal spaces by applying wavelet transform. Then, after applying general tensor discriminant analysis (GTDA) on tensors and calculating mapping matrix, feature vectors are extracted. GTDA increases the sensitivity of the algorithm by storing data without deleting them. Finally, K-Nearest neighbors (KNN) is used to classify the selected features. Results The results of simulating algorithm on algorithm standard dataset shows that the algorithm is capable of detecting 98 percent of seizures with an average delay of 4.7 seconds and the average error rate detection of three errors in 24 hours. Conclusion Today, the lack of an automated system to detect or predict the seizure onset is strongly felt. PMID:27672628

  18. Anatomy and Physiology in Health and Illness - Text, Colouring Book and Workbook Package:Tenth edition Anne Waugh and Allison Grant Anatomy and Physiology in Health and Illness - Text, Colouring Book and Workbook Package:Tenth edition Elsevier 490pp plus 285pp £39.99 for the two 044310416 044310416 [Formula: see text].

    PubMed

    2006-10-11

    There are many useful anatomy and physiology texts on the market but few address adequately the needs of the undergraduate student of nursing science. However, this new edition by Ross and Wilson proves a valuable exception, appealing to those with little or no scientific background and more experienced students wishing to enhance their knowledge of biomedical principles.

  19. Interstitial Spaces: Visual Culture, Domesticity, and Metaphor

    ERIC Educational Resources Information Center

    Sanders, James H., III; Buenger, Allison

    2010-01-01

    Allison Buenger graduated from the Ohio State University with a bachelor of fine arts degree in ceramics in December 2009. Her works morph domestic objects into vessels of meaning, sculptures displayed in larger installations of textiles and found objects. Originally from Northeast Ohio, Allison resides in Columbus, Ohio, and works at Open Door…

  20. Welcoming Nora: A Family Event

    PubMed Central

    Walsh, Allison J.; Walsh, Paul R.; Walsh, Jane M.; Walsh, Gavin T.

    2011-01-01

    In this column, Allison and Paul Walsh share the story of the birth of Nora, their third baby and their second child to be born at home. Allison and Paul share their individual memories of labor and birth. But their story is only part of the story of Nora’s birth. Nora’s birth was a family event, with Allison and Paul’s other children very much part of the experience. Jane and Gavin share their own memories of their baby sister’s birth. PMID:22654460

  1. Civilian Police: Future of the Military Police Corps

    DTIC Science & Technology

    2009-02-20

    Station Security Allison Choike, a reporter with Ft. Leonard Wood, Missouri’s The Guidon , writes that the concept of hiring civilians to fill the law...6 Allison Choike, “Fort Leonard Wood Trains DA Installation Civilian Police,” The Guidon , 5 June 2008, <www.myguidon.com/index2.php?option...Bibliography Choike, Allison. “Fort Leonard Wood Trains DA Installation Civilian Police.” The Guidon . 5 June 2008. <http://www.myguidon.com/index2.php

  2. Sensor Technology Baseline Study for Enabling Condition Based Maintenance Plus in Army Ground Vehicles

    DTIC Science & Technology

    2012-03-01

    high cost driver components in Army ground vehicles as identified by TARDEC are engines, transmissions, batteries, and alternators . This report...7 4.3 Alternator .......................................................................................................................12...C7 diesel engine. ...........................................................................................4 Figure 2. Allison transmission 2500 SP

  3. 10. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF GUEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF GUEST BEDROOM, UNUSUAL PARQUET FLOORS AND PINK MARBLE FIREPLACE Original in Collections of Marian College - James A. Allison Mansion, 3200 Coldspring Road, Indianapolis, Marion County, IN

  4. 9. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF BEDROOMQUALITY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF BEDROOM-QUALITY CARVED WHITE MAHOGANY MOLDING AND FIREPLACE MANTLE WHICH SURROUNDS MARBLE FIREPLACE Original in Collections of Marian College - James A. Allison Mansion, 3200 Coldspring Road, Indianapolis, Marion County, IN

  5. Meprobamate overdose

    MedlinePlus

    Benitez JG, Allison LG, Ternullo S. Sedative-hypnotics. In: Shannon MW, Borron SW, Burns MJ, eds. Haddad and Winchester's Clinical Management of Poisoning and Drug Overdose . 4th ed. Philadelphia, PA: ...

  6. 75 FR 81854 - Safety Zone; New Year's Celebration for the City of San Francisco, Fireworks Display, San...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-29

    ... this temporary rule, call Lieutenant Junior Grade Allison Natcher at 415- 399-7442, or e-mail D11-PF..., in the aggregate, or by the private sector of $100,000,000 (adjusted for inflation) or more in...

  7. 75 FR 47626 - Notice of Reestablishment of the Pinedale Anticline Working Group, Wyoming

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-06

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE INTERIOR Bureau of Land Management Notice of Reestablishment of the Pinedale Anticline Working Group, Wyoming... Management's (BLM) Pinedale Anticline Working Group (PAWG). FOR FURTHER INFORMATION CONTACT: Allison...

  8. Advanced turbine systems program. Quarterly report, February 1--April 30, 1996

    SciTech Connect

    1996-12-31

    Allison continued progress on the following tasks during this quarter: (distributed generation) market study, GFATS system definition and analysis, Castcool{trademark} blade technology demonstration, low emissions combustion system, ceramic vane design and evaluation, and program management.

  9. 8. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF SLEEPING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF SLEEPING PORCH, AT NORTH END CAPABLE OF SLEEPING SIX. Original in Collections of Marian College - James A. Allison Mansion, 3200 Coldspring Road, Indianapolis, Marion County, IN

  10. Task 6 -- Advanced turbine systems program conceptual design and product development

    SciTech Connect

    1996-01-10

    The Allison Engine Company has completed the Task 6 Conceptual Design and Analysis of Phase 2 of the Advanced Turbine System (ATS) contract. At the heart of Allison`s system is an advanced simple cycle gas turbine engine. This engine will incorporate components that ensure the program goals are met. Allison plans to commercialize the ATS demonstrator and market a family of engines incorporating this technology. This family of engines, ranging from 4.9 MW to 12 MW, will be suitable for use in all industrial engine applications, including electric power generation, mechanical drive, and marine propulsion. In the field of electric power generation, the engines will be used for base load, standby, cogeneration, and distributed generation applications.

  11. Genetic Testing for Inherited Heart Disease

    MedlinePlus

    ... of the American Heart Association Cardiology Patient Page Genetic Testing for Inherited Heart Disease Allison L. Cirino , ... for developing the family’s heart condition. What Is Genetic Testing and What Can it Tell Me? Genetic ...

  12. Ebola (Ebola Virus Disease): Q&As on Transmission

    MedlinePlus

    ... Other African Countries Partnering with West African Communities Call CDC's Role Photos From the Field CDC Responder Stories Allison Friedman Gary Cobb, Arthur Hudson, and Drenda Morrissette John Saindon and Brian Bird Karen Wong Angela Dunn Brant Goode CDC In ...

  13. Statins and Risk of New-Onset Diabetes Mellitus

    MedlinePlus

    ... Patient Page Statins and Risk of New-Onset Diabetes Mellitus Ravi V. Shah , Allison B. Goldfine Download ... initiation in at-risk patients. Can Statins Cause Diabetes Mellitus? Careful review of findings from many trials ...

  14. 76 FR 464 - Notification of U.S. Fish Quotas and an Effort Allocation in the Northwest Atlantic Fisheries...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-05

    ... Great Republic Drive, Gloucester, MA 01930 (phone: 978- 281-9103, fax: 978-281-9135, e-mail: allison..., autonomous vessel monitoring system; and adherence to all relevant minimum size, gear, bycatch, and...

  15. Simulation of the Migration, Fate, and Effects of Diazinon in Two Monticello Stream Channels,

    DTIC Science & Technology

    1981-12-01

    roaming fish, three cages with a total of 200 minnows per channel were placed in the first pool within the study area. About 10% of the free- swimming fish...of eggs has been shown in much lower concentrations for fathad minnows. Spinal scoliosis has been diagnosed by Allison and Hermanutz (1977) at a...at 90 pg/l Pimephales promelas Reduced hatching and increase in Allison and (fathead minnow) incidence of scoliosis at 3.2 wg/l Hermanutz 1977

  16. Paradox or Prudence? Analyzing the Coherence of the US’ China Strategy

    DTIC Science & Technology

    2013-06-01

    Peace, 211. 17 Doyle, Ways of War and Peace, 211. 18 Doyle, Ways of War and Peace, 210-211. See also Alexander Wendt, Social Theory of...diverge, competition has the potential to become tension and tension has the potential to become conflict. Graham Allison and Robert Blackwill...46 Graham Allison and Robert D. Blackwill, "Will China Ever Be No. 1?" Foreign Policy.com, February 16, 2013, http

  17. Afghanistan in the Balance: Air Politik

    DTIC Science & Technology

    2010-06-01

    However, one must acknowledge that alternative explanations exist. In their study of the Cuban Missile Crisis, Graham T. Allison and Philip Zelikow...Graham T. Allison and Philip Zelikow, Essence of Decision: Explaining the Cuban Missile Crisis, 2nd ed. (New York: Longman, 1999). Instructively...treasure.html. See also: Lindsay Hodges Anderson, "Debate on Afghanistan Strategy Continues among Hks Experts," Harvard Kennedy School of

  18. Recapitalizing Nuclear Weapons (Walker Paper, Number 8)

    DTIC Science & Technology

    2007-08-01

    Information Warfare and later executive officer for the Direc- tor of Operations at Headquarters Air Mobility Command (AMC), Scott AFB, Illinois... Allison Mc- farlane posits that all WMD are not equal and urges caution in establishing US nuclear weapons policy against the generic term WMD.13...11 August 2004, 2–3. 13. Allison Mcfarlane, “All Weapons of Mass Destruction are not Equal,” MIT Center for International Studies, Audit of

  19. Silicon-Based Nanoscale Composite Energetic Materials

    DTIC Science & Technology

    2013-02-01

    February 2013 HDTRA1-08-1-0006 Steven F. Son et al. Prepared by: Purdue University 130 Chaffee Hall 500 Allison Road West...Richard A. Yetter, and Alexander S. Mukasyan Purdue University 130 Chaffee Hall 500 Allison Road West Lafayette, IN 47907 Defense Threat Reduction...Supported: This project partially supported three faculty members (Richard Yetter, Alex Mukasyan and Steven Son). Two graduate students (one at Purdue

  20. Cellular Neural Network Models of Growth and Immune of Effector Cells Response to Cancer

    NASA Astrophysics Data System (ADS)

    Su, Yongmei; Min, Lequan

    Four reaction-diffusion cellular neural network (R-D CNN) models are set up based on the differential equation models for the growths of effector cells and cancer cells, and the model of the immune response to cancer proposed by Allison et al. The CNN models have different reaction-diffusion coefficients and coupling parameters. The R-D CNN models may provide possible quantitative interpretations, and are good in agreement with the in vitro experiment data reported by Allison et al.

  1. Rapid detection of the factor XIII Val34Leu (163 G-->T) polymorphism by real-time PCR using fluorescence resonance energy transfer detection and melting curve analysis.

    PubMed

    Shemirani, Amir H; Muszbek, László

    2004-01-01

    The Val34Leu polymorphism in the A subunit of blood coagulation factor XIII (FXIII-A) is located in the activation peptide, just three amino acids upstream of the thrombin cleavage site. The Val-->Leu replacement accelerates the rate of the proteolytic activation of FXIII and it seems to provide protection against myocardial infarction. Methods available for the assessment of the FXIII-A Val34Leu polymorphism are rather time-consuming, laborious and not easily applicable for large-scale studies. In this study a new method based on real-time PCR with fluorescence resonance energy transfer (FRET) detection and melting curve analysis was developed. The rapid, simple method was adapted to the widely used real-time PCR instrument, LightCycler (Roche Diagnostics). The results showed 100% coincidence with those obtained by the traditional PCR-restriction fragment length polymorphism (RFLP) assay and fluorescent DNA sequencing. Using this method, an allele frequency of 24.2% was obtained (n=113), which well agrees with the allele frequency obtained by PCR-RFLP on a different group of the same ethnic Hungarian population (25.9%).

  2. Corrigendum to "Testing models of thorium and particle cycling in the ocean using data from station GT11-22 of the U.S. GEOTRACES North Atlantic Section" [Deep-Sea Res. I 113 (2016) 57-79

    NASA Astrophysics Data System (ADS)

    Lerner, Paul; Marchal, Olivier; Lam, Phoebe J.; Anderson, Robert F.; Buesseler, Ken; Charette, Matthew A.; Edwards, R. Lawrence; Hayes, Christopher T.; Huang, Kuo-Fang; Lu, Yanbin; Robinson, Laura F.; Solow, Andrew

    2016-12-01

    The authors regret a small number of numerical errors in their paper. The values for the slopes reported in the caption of Figure 14 currently reads: '(a) 0.81±0.06, (b) 0.06±0.03, (c) 0.73±0.06, and (d) -0.28±0.06'. These values were derived from earlier calculations of these slopes that were later revised. The correct values (in italics in this corrigendum) are '(a) 0.60±0.05, (b) -0.06±0.04, (c) 0.64±0.06, and (d) -0.36±0.06'. Incorrect values of the slopes are also reported in the main text of the paper, and corrections to the main text are specified below. The panels in Figure 14 are correct, and refer to the revised values for the slopes.

  3. Large structural modulations in the relaxor ferroelectric and intermediate state of strontium rich members (x&2gt;0.6) of the SrxBa1-xNb2O6 (sbn) solid solution series

    NASA Astrophysics Data System (ADS)

    Graetsch, H. A.

    2017-02-01

    The amplitudes of the positional, occupational and adp modulations of sbn mixed crystals are strongly enhanced for high strontium contents. The increase of structural modulations is accompanied by reduced spontaneous electric polarization largely due to smaller off-center shifts of the niobium atoms. Beyond the room temperature ferroelectric - intermediate transition near x=0.77, anomal large U33 atomic displacement parameters of the niobium atoms indicate static disorder caused by loss of orientational coupling between residual shifts of Nb atoms in neighboring NbO6 octahedra. Change of satellite intensities show a reduction from two-dimensional to one-dimensional modulation which is not consistent with tetragonal symmetry. The pseudo-tetragonally twinned crystal structure of sbn82 was refined in the orthorhombic super-space group A2mm(½0γ)000. The apparent tetragonal symmetry of the other investigated sbn samples also seems to be due to pseudo tetragonal twinning with equal twin volumes. The modulations mainly consist of cooperatively tilted NbO6 octahedra and wave-like ordered incomplete occupation of the largest cation sites (Me2a and b) by Ba2+ and Sr2+. Furthermore, the atomic displacement parameters of the Me2 sites are strongly modulated.

  4. Advanced Turbine Systems Program. Topical report

    SciTech Connect

    1993-03-01

    The Allison Gas Turbine Division (Allison) of General Motors Corporation conducted the Advanced Turbine Systems (ATS) program feasibility study (Phase I) in accordance with the Morgantown Energy Technology Center`s (METC`s) contract DE-AC21-86MC23165 A028. This feasibility study was to define and describe a natural gas-fired reference system which would meet the objective of {ge}60% overall efficiency, produce nitrogen oxides (NO{sub x}) emissions 10% less than the state-of-the-art without post combustion controls, and cost of electricity of the N{sup th} system to be approximately 10% below that of the current systems. In addition, the selected natural gas-fired reference system was expected to be adaptable to coal. The Allison proposed reference system feasibility study incorporated Allison`s long-term experience from advanced aerospace and military technology programs. This experience base is pertinent and crucial to the success of the ATS program. The existing aeroderivative technology base includes high temperature hot section design capability, single crystal technology, advanced cooling techniques, high temperature ceramics, ultrahigh turbomachinery components design, advanced cycles, and sophisticated computer codes.

  5. Greater trochanter location measurement using a three-dimensional motion capture system during prone hip extension

    PubMed Central

    Yu, Ji-Su; Oh, Jae-Seop

    2017-01-01

    [Purpose] The greater trochanter (GT) is an important structure in biomedical research, but the measurement methods require development. This study presents data from a new measurement method that does not use GT-marker-based measurement (No GT-m) in comparison with GT-marker based measurement (GT-m). [Subjects and Methods] We recruited 20 healthy subjects, who were asked to perform and maintain a prone position and then move to the prone hip extension. A motion capture system collected the kinematic data and the location of the GT was calculated by two measurements. [Results] GT migration distance differed significantly between the two measurements and the coefficient of the variation value was lower for the No GT-m method. Thigh lengths of the No GT-m method were comparable to the original lengths. There were significant differences between the GT-m and the other methods. [Conclusions] These data suggest that the GT-m method yielded a lower precision with a smaller GT migration distance. In the comparison of thigh length, the No GT-m method was in close agreement with the original length. We suggest that determining the location of the GT using the No GT-m has greater accuracy than the GT-m method. PMID:28265151

  6. A Novel Glucosylation Reaction on Anthocyanins Catalyzed by Acyl-Glucose–Dependent Glucosyltransferase in the Petals of Carnation and Delphinium[C][W

    PubMed Central

    Matsuba, Yuki; Sasaki, Nobuhiro; Tera, Masayuki; Okamura, Masachika; Abe, Yutaka; Okamoto, Emi; Nakamura, Haruka; Funabashi, Hisakage; Takatsu, Makoto; Saito, Mikako; Matsuoka, Hideaki; Nagasawa, Kazuo; Ozeki, Yoshihiro

    2010-01-01

    Glucosylation of anthocyanin in carnations (Dianthus caryophyllus) and delphiniums (Delphinium grandiflorum) involves novel sugar donors, aromatic acyl-glucoses, in a reaction catalyzed by the enzymes acyl-glucose–dependent anthocyanin 5(7)-O-glucosyltransferase (AA5GT and AA7GT). The AA5GT enzyme was purified from carnation petals, and cDNAs encoding carnation Dc AA5GT and the delphinium homolog Dg AA7GT were isolated. Recombinant Dc AA5GT and Dg AA7GT proteins showed AA5GT and AA7GT activities in vitro. Although expression of Dc AA5GT in developing carnation petals was highest at early stages, AA5GT activity and anthocyanin accumulation continued to increase during later stages. Neither Dc AA5GT expression nor AA5GT activity was observed in the petals of mutant carnations; these petals accumulated anthocyanin lacking the glucosyl moiety at the 5 position. Transient expression of Dc AA5GT in petal cells of mutant carnations is expected to result in the transfer of a glucose moiety to the 5 position of anthocyanin. The amino acid sequences of Dc AA5GT and Dg AA7GT showed high similarity to glycoside hydrolase family 1 proteins, which typically act as β-glycosidases. A phylogenetic analysis of the amino acid sequences suggested that other plant species are likely to have similar acyl-glucose–dependent glucosyltransferases. PMID:20971893

  7. A novel glucosylation reaction on anthocyanins catalyzed by acyl-glucose-dependent glucosyltransferase in the petals of carnation and delphinium.

    PubMed

    Matsuba, Yuki; Sasaki, Nobuhiro; Tera, Masayuki; Okamura, Masachika; Abe, Yutaka; Okamoto, Emi; Nakamura, Haruka; Funabashi, Hisakage; Takatsu, Makoto; Saito, Mikako; Matsuoka, Hideaki; Nagasawa, Kazuo; Ozeki, Yoshihiro

    2010-10-01

    Glucosylation of anthocyanin in carnations (Dianthus caryophyllus) and delphiniums (Delphinium grandiflorum) involves novel sugar donors, aromatic acyl-glucoses, in a reaction catalyzed by the enzymes acyl-glucose-dependent anthocyanin 5(7)-O-glucosyltransferase (AA5GT and AA7GT). The AA5GT enzyme was purified from carnation petals, and cDNAs encoding carnation Dc AA5GT and the delphinium homolog Dg AA7GT were isolated. Recombinant Dc AA5GT and Dg AA7GT proteins showed AA5GT and AA7GT activities in vitro. Although expression of Dc AA5GT in developing carnation petals was highest at early stages, AA5GT activity and anthocyanin accumulation continued to increase during later stages. Neither Dc AA5GT expression nor AA5GT activity was observed in the petals of mutant carnations; these petals accumulated anthocyanin lacking the glucosyl moiety at the 5 position. Transient expression of Dc AA5GT in petal cells of mutant carnations is expected to result in the transfer of a glucose moiety to the 5 position of anthocyanin. The amino acid sequences of Dc AA5GT and Dg AA7GT showed high similarity to glycoside hydrolase family 1 proteins, which typically act as β-glycosidases. A phylogenetic analysis of the amino acid sequences suggested that other plant species are likely to have similar acyl-glucose-dependent glucosyltransferases.

  8. Comparisons and Analyses of Gifted Students' Characteristics and Learning Methods

    ERIC Educational Resources Information Center

    Lu, Jiamei; Li, Daqi; Stevens, Carla; Ye, Renmin

    2017-01-01

    Using PISA 2009, an international education database, this study compares gifted and talented (GT) students in three groups with normal (non-GT) students by examining student characteristics, reading, schooling, learning methods, and use of strategies for understanding and memorizing. Results indicate that the GT and non-GT gender distributions…

  9. A dc Penning Surface-Plasma Source

    DTIC Science & Technology

    2007-11-02

    LA-UR-93-2990 Title: A dc Penning Surface-Plasma Source Author(s): Submitted to: H. Vernon Smith, Jr., Paul Allison, Carl Geisik, David R...Type: HC Number of Copies In Library: 000001 Record ID: 28620 A de Penning Surface-Plasma Source* H. Vernon Smith, Jr., Paul Allison, Carl Geisik...Schechter, J. H. Whealton, and J. J. Donaghy, ATP Conf. Proc. No. 158, 366 (1987). 8) H. V. Smith, Jr., N. M. Schnurr, D. H. Whitaker , and K. E. Kalash

  10. Design and Construction of an Ultra-Low-Background 14 Germanium Crystal Array for High Efficiency and Coincidence Measurements

    DTIC Science & Technology

    2008-09-01

    performance and measurement of mixed fission products were conducted using the GEANT4 (G4) framework (Agostinelli 2003, Allison 2006). A diagram of the...P. Arce (2003). Geant4 —a simulation toolkit, Nucl. Inst. Meth. A 506: 250–303. Allison, J., K. Amako, J. Apostolakis, H. Araujo, P. A. Dubois, and...M. Asai (2006). Geant4 developments and application, IEEE Trans. Nucl. Sci. 53: 270–278. Brodzinski R. L., H.S. Miley, H. J. Reeves, F. T. Avignone

  11. Pollution and Climate Change

    ERIC Educational Resources Information Center

    Larr, Allison S.; Neidell, Matthew

    2016-01-01

    Childhood is a particularly sensitive time when it comes to pollution exposure. Allison Larr and Matthew Neidell focus on two atmospheric pollutants--ozone and particulate matter--that can harm children's health in many ways. Ozone irritates the lungs, causing various respiratory symptoms; it can also damage the lung lining or aggravate lung…

  12. Garden Variety Experiential Education: The "Material Turn" and Environmental Ethics

    ERIC Educational Resources Information Center

    Wallace, Allison B.

    2016-01-01

    Allison B. Wallace describes a seminar in organic horticulture she created and teaches as part of an honors curriculum. She answers the question of how gardening is appropriate for high-ability college students by saying that she believes efforts to raise plants by relatively non violent means teaches and disciplines students in an ethical way to…

  13. Antineoplastic Efficacy of Novel Polyamine Analogues in Human Breast Cancer

    DTIC Science & Technology

    2005-06-01

    Allison Pledgie‡, Yi Huang‡, Amy Hacker‡, Zhe Zhang‡, Patrick M. Woster§, Nancy E. Davidson‡1, and Robert A. Casero, Jr.‡2 From the ‡Sidney Kimmel...21. Tang , Z., Treilleux, I., and Brown, M. (1997) Mol. Cell. Biol. 17, 1274–1280 22. Haworth, I. S., Rodger, A., and Richards, W. G. (1991) Proc. Biol

  14. 6. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF DINING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF DINING ROOM, LOOKING NORTHWEST. ORIGINAL VELOUR IS STILL ON WALLS AND IN GOOD CONDITION. CARVINGS ON FIREPLACE FEATURE A FRUIT MOTIF. FLOORS PARQUET Original in Collections of Marian College - James A. Allison Mansion, 3200 Coldspring Road, Indianapolis, Marion County, IN

  15. "In the End, Our Message Weighs": "Blood Run," NAGPRA, and American Indian Identity

    ERIC Educational Resources Information Center

    Kelsey, Penelope; Carpenter, Cari M.

    2011-01-01

    In this article, the authors juxtapose Allison Hedge Coke's poetry collection "Blood Run" (2006) with the larger context in which Native American Graves Protection and Repatriation Act (NAGPRA) operates in order to investigate how "Blood Run" exposes the limitations of repatriation legislation, most significantly, how NAGPRA's…

  16. Identifying the Core Content and Structure of a Schema for Cultural Understanding

    DTIC Science & Technology

    2009-06-01

    Technical Report 1251 Identifying the Core Content and Structure of a Schema for Cultural Understanding Joan R...333 6. AUTHOR(S): Joan Rentsch and Iona Mot (Organizational Research Group) Allison Abbe (U.S. Army Research Institute) 5e. WORK UNIT...iii Technical Report 1251 Identifying the Core Content and Structure of a Schema for Cultural Understanding Joan R. Rentsch

  17. Finding Center: How Learning Centers Evolved in a Secondary Student-Centered Classroom

    ERIC Educational Resources Information Center

    Movitz, Allison P.; Holmes, Kerry P.

    2007-01-01

    The authors elaborate on the experience of creating for high school students effective multisensory, hands-on learning centers that address a full range of elements from the English language arts curriculum. Allison P. Movitz and Kerry P. Holmes detail the centers Movitz designed for a Mostly Medieval unit to show how learning centers can help…

  18. Association of AKI with Adverse Outcomes in Burned Military Casualties

    DTIC Science & Technology

    2012-02-01

    a baseline de- rived by solving the Modification of Diet in Renal Disease (MDRD) study equation assuming an estimated GFR of 75 ml/min per 1.73 m2...McClellan WM, Herzog CA, Kiefe CI, Eggers PW, Allison JJ: Long term risk of mortality and end stage renal disease among the elderly after small increases

  19. The Effects of Age, Years of Experience, and Type of Experience in the Teacher Selection Process

    ERIC Educational Resources Information Center

    Place, A. William; Vail, David S.

    2013-01-01

    Paper screening in the pre-selection process of hiring teachers has been an established line of research starting with Young and Allison (1982). Administrators were asked to rate hypothetical candidates based on the information provided by the researcher. The dependent variable in several of these studies (e.g. Young & Fox, 2002; Young & Schmidt,…

  20. The Creative Child

    ERIC Educational Resources Information Center

    Leader, Wendy

    2012-01-01

    Jen and Michael agreed that it felt like a mixed blessing when the kindergarten teacher told them she thought their daughter was "very creative." The teacher had commented on Allison's love of dramatic and imaginative play, her bold and expressive artwork, her knack for solving problems with numbers and shapes, and her delight in finding…

  1. 75 FR 66390 - Notice of Lodging of Settlement Agreement Under the Comprehensive Environmental Response...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-28

    ... Allison Gas Turbines Site in Indiana; 9. The Venture 2000 Property Site in Indiana; 10. The 1-Acre Fire... Site, a/k/a the Massena Site, in New York; 76. The GM-IFG Syracuse Site in New York; 77. The Ley...

  2. The Resilient Leader

    ERIC Educational Resources Information Center

    Allison, Elle

    2012-01-01

    School leaders currently face so many challenges--some as basic as a lack of money to hire enough teachers--that they know they need to increase their resilience. According to Allison, who coaches school leaders, strong leaders know how important maintaining resilience is. They recognize when their reserves of hope--and those of their…

  3. RESPIRATORY HEALTH OF RURAL AND FARM WOMEN IN THE KEOKUK COUNTY RURAL HEALTH STUDY

    EPA Science Inventory

    RESPIRATORY HEALTH OF RURAL AND FARM WOMEN IN THE KEOKUK COUNTY RURAL HEALTH STUDY
    Allison L. Naleway*, Nancy L. Sprince?, Erik R. Svendsen?, Ann M. Stromquist?, James A. Merchant?
    *Marshfield Medical Research and Education Foundation, Marshfield, WI; ?University of Iowa Co...

  4. Investigating Move Structure of English Applied Linguistics Research Article Discussions Published in International and Thai Journals

    ERIC Educational Resources Information Center

    Amnuai, Wirada; Wannaruk, Anchalee

    2013-01-01

    This study investigates the rhetorical move structure of English applied linguistic research article Discussions published in Thai and international journals. Two corpora comprising of 30 Thai Discussions and 30 international Discussions were analyzed using Yang & Allison's (2003) move model. Based on the analysis, both similarities and…

  5. A Portrait of Two Writing Teachers.

    ERIC Educational Resources Information Center

    Goldberg, Mark F.

    1990-01-01

    Profiles Andre Allison and Florence Mondry, English teachers at Shoreham-Wading River High School (New York), who have placed composition at the center of their curricula, giving students more responsibility and fostering cooperative learning. Both use reading logs to help students interpret literature intelligently and extract meaning from their…

  6. New Media and Technology. New Directions for Youth Development, No. 128

    ERIC Educational Resources Information Center

    Bers, Marina Umaschi

    2011-01-01

    This volume contains the following papers: (1) Beyond Computer Literacy: Supporting Youth's Positive Development through Technology (Marina Umaschi Bers); (2) Educational Technology, Reimagined (Michael Eisenberg); (3) Children as Codesigners of New Technologies: Valuing the Imagination to Transform What Is Possible (Allison Druin); (4) Content…

  7. Hong Kong Papers in Linguistics and Language Teaching, Number 17.

    ERIC Educational Resources Information Center

    Benson, Phil, Ed.; And Others

    1994-01-01

    Seven articles on linguistics and language teaching, two book reviews, and four conference summaries are included in this volume. The articles include: "Laying Down the Law? Reflecting on Course Design in Progress" (Desmond Allison, Robin Corcos, Agnes Lam); "Beyond (F)utilitarianism: English as an Academic Purpose" (Alastair…

  8. 75 FR 52736 - Environmental Impacts Statements; Notice of Availability

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-27

    ..., Contact: Allison Shaffer 760-833-7100. EIS No. 20100339, Final EIS, BLM, CA, Genesis Solar Energy Project... Florida Keys and Flower Garden Banks National Marine Sanctuaries, Implementation, FL, TX, and LA, Wait... Solar Desert Sunlight Solar Farm (DSSF) Project, Proposing To Develop a 550-Megawatt Photovoltaic...

  9. Advanced turbine systems program

    SciTech Connect

    Wilkes, C.; Mukavetz, D.W.; Knickerbocker, T.K.; Ali, S.A.

    1992-12-31

    In accordance with the goals of the DOE program, improvements in the gas turbine are the primary focus of Allison activity during Phase I. To this end Allison conducted a survey of potentially applicable gas turbine cycles and selected the advanced combined cycle as reference system. Extensive analysis of two versions of the advanced combined cycle was performed against the requirement for a 60% thermal efficiency (LHV) utility-sized, natural gas fired system. This analysis resulted in technology requirements for this system. Additional analysis determined emissions potential for the system, established a coal-fueled derivative system and a commercialization plan. This report deals with the technical requirements for a system that meets the thermal efficiency goal. Allison initially investigated four basic thermodynamic cycles: Humid air turbine, intercalate-recuperated systems, advanced combined cycle, chemically recuperated cycle. Our survey and cycle analysis indicated that au had the potential of reaching 60% thermal efficiency. We also concluded that engine hot section technology would be a critical technology regardless of which cycle was chosen. Based on this result Allison chose to concentrate on the advanced combined cycle. This cycle is well known and understood by the utility turbine user community and is therefore likely to be acceptable to users.

  10. Advanced turbine systems program

    SciTech Connect

    Wilkes, C.; Mukavetz, D.W.; Knickerbocker, T.K.; Ali, S.A.

    1992-01-01

    In accordance with the goals of the DOE program, improvements in the gas turbine are the primary focus of Allison activity during Phase I. To this end Allison conducted a survey of potentially applicable gas turbine cycles and selected the advanced combined cycle as reference system. Extensive analysis of two versions of the advanced combined cycle was performed against the requirement for a 60% thermal efficiency (LHV) utility-sized, natural gas fired system. This analysis resulted in technology requirements for this system. Additional analysis determined emissions potential for the system, established a coal-fueled derivative system and a commercialization plan. This report deals with the technical requirements for a system that meets the thermal efficiency goal. Allison initially investigated four basic thermodynamic cycles: Humid air turbine, intercalate-recuperated systems, advanced combined cycle, chemically recuperated cycle. Our survey and cycle analysis indicated that au had the potential of reaching 60% thermal efficiency. We also concluded that engine hot section technology would be a critical technology regardless of which cycle was chosen. Based on this result Allison chose to concentrate on the advanced combined cycle. This cycle is well known and understood by the utility turbine user community and is therefore likely to be acceptable to users.

  11. Re-Vitalizing the First Year Class through Student Engagement and Discovery Learning

    ERIC Educational Resources Information Center

    Steuter, Erin; Doyle, Judith

    2010-01-01

    The first year course in Sociology at Mount Allison University introduced students to social issues via dynamic class interactions and assignments that are designed to build conceptual and applied skills. Developments to the course organization have maximized the opportunities for discovery learning and have made the class an enjoyable teaching…

  12. Using Clustering Strategies for Creating Authority Files

    DTIC Science & Technology

    2000-06-01

    Using Clustering Strategies for Creating Authority Files James C. French* and Allison L. Powell Department of Computer Science, University of...differences will all increase the difficulty of retriev- ing information. We investigate a number of approximate string matching techniques that have...accurate information. As the pace of electronic publication accelerates, there will be expand- ing reliance on automated techniques to aid information

  13. Hongkong Papers in Linguistics and Language Teaching, 14.

    ERIC Educational Resources Information Center

    Allison, Desmond M., Ed.; Leung, Lily S. K., Ed.

    1991-01-01

    Papers on linguistics and language teaching in this volume are the following: "'Good' and 'Poor' Writing and Writers: Studying Individual Performance as a Part of Test Validation" (Desmond Allison, Evelyn Cheung); "To Test or Not To Test: That Is the Question" (Keith Tong, Rose Chan, Jo Lewkowicz); "Testing Listening…

  14. No Space for Girliness in Physics: Understanding and Overcoming the Masculinity of Physics

    ERIC Educational Resources Information Center

    Götschel, Helene

    2014-01-01

    Allison Gonsalves' article on "women doctoral students' positioning around discourses of gender and competence in physics" explores narratives of Canadian women physicists concerning their strategies to gain recognition as physicists. In my response to her rewarding and inspiring analysis I will reflect on her findings and arguments and…

  15. Department of the Navy, Justification of Estimates for Fiscal Year 1984 Submitted to Congress January 1983, Procurement, Other Procurement, Navy.

    DTIC Science & Technology

    1983-01-01

    and FFG-7 Class construction programs and the Allison 501K Gas Turbine engine introduced into the Fleet through the DD-963, DDG - 993 , and DD-997 Class...Funds are also required for maintenance items and newly developed improvements such as the AN/WSN-5 Inertial Navigation Sets for CG/CGN/ DDG Class

  16. Talking about Math

    ERIC Educational Resources Information Center

    Hintz, Allison; Kazemi, Elham

    2014-01-01

    By simply asking students how they got the answer to a problem, math teachers can open the door to stimulating discussions in class. But how can they make sure those discussions promote mathematical understanding? Allison Hintz and Elham Kazemi explain that teachers need to know what the goal is for a discussion and plan accordingly. The authors…

  17. Early Childhood Education: Pathways to Better Health. Preschool Policy Brief Issue 25

    ERIC Educational Resources Information Center

    Friedman-Krauss, Allison; Barnett, W. Steven

    2013-01-01

    The potential health benefits of early childhood education programs are quite large, especially for children living in poverty. In this report, authors Allison Friedman-Krauss and Steve Barnett set out the evidence regarding the short and long term health benefits to children from early childhood education programs, identify the features of…

  18. A Beta-Amino Acid Pyrokinin Analog Induces Irregular Pupariation Behavior in Larvae of the Flesh Fly Sarcophaga bullata

    DTIC Science & Technology

    2008-01-01

    Allison STREY1, and Jan ZDAREK3* 1Areawide Pest Management Research, Southern Plains Agricultural Research Center, USDA, 2881 F/B Road, College...Areawide Pest Management Research,Southern Plains Agricultural Research Center, USDA,2881 F/B Road,College Station,TX,77845 8. PERFORMING ORGANIZATION

  19. Colleges Are Slashing Adjuncts' Hours to Skirt New Rules on Health-Insurance Eligibility

    ERIC Educational Resources Information Center

    Dunn, Sydni

    2013-01-01

    Allison G. Armentrout, an adjunct instructor at Stark State College, does not get paid by the hour. She earns $4,600 to teach two English composition courses. But now she carefully tracks how many hours she works on an electronic time sheet. During a recent week, she spent three hours preparing for her lectures, close to six hours in the…

  20. Sensor Systems for Biological Agent Attacks: Protecting Buildings and Military Bases

    DTIC Science & Technology

    2004-01-01

    for Advanced Sensors Board on Manufacturing and Engineering Design Division on Engineering and Physical Sciences...PROCESSES FOR ADVANCED SENSORS JOHN VITKO, JR., Sandia National Laboratories, Livermore, California, Chair DAVID R. FRANZ, Midwest Research Institute...Michigan ANGELO M. NINIVAGGI, JR., Plexus Corporation, Nampa, Idaho JAMES B. O’DWYER, PPG Industries , Allison Park, Pennsylvania HERSCHEL REESE

  1. Utilization Research. Chapter 11.

    ERIC Educational Resources Information Center

    1996

    This collection of papers presented at a 1996 conference on children's mental health focuses on utilization research. Papers have the following titles and authors: (1) "Information Equity: A Critical Component of Strong Service Systems" (Catherine Batsche and Allison Metcalf); (2) "Utilization of Children's Mental Health Services: Differentiating…

  2. Special Edition. Volume 1, Issue III, June 2004

    ERIC Educational Resources Information Center

    McDonough, Benton, Ed.

    2004-01-01

    This issue of "Special Edition" contains the following articles: (1) Message from the Assistant Commissioner (Joseph Fisher); (2) Inclusion: Success in the Classroom (Benton McDonough); (3) Mid-Tennessee (Allison Davey); (4) West Tennessee (Larry Greer); (5) East Tennessee (Robert Winstead); (6) Excellence in Teaching (Paul Ayers and…

  3. Cross-Cultural Nonverbal Cue Immersive Training

    DTIC Science & Technology

    2008-12-01

    1 CROSS-CULTURAL NONVERBAL CUE IMMERSIVE TRAINING Shatha N. Samman*, Michael Moshell + , Bryan Clark, Chantel Brathwaite + , and Allison Abbe...their meaning. 1.2 Nonverbal Cues Categorized by Function In one commonly accepted taxonomic approach, Ekman and Friesen (1969) classified...nonverbal cues (Ekman & Friesen , 1969). Emblems occur mainly when verbal communication is inhibited by external factors (e.g., noise, distance

  4. 77 FR 17566 - Notice of Proposed Buy America Waivers

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-26

    ... December 31, 2013 so they may complete their ongoing process to secure a domestic supplier of Lithium Ion... Lithium Ion batteries. The ESU is one of five subsystems of Allison's hybrid-electric propulsion system... competitive assessments, technical reviews, and independent market studies with U.S. based Lithium...

  5. The Modernization of Signs: A Library Leads the Way to Networked Digital Signage

    ERIC Educational Resources Information Center

    Larson, Kendall; Quam, Allison

    2010-01-01

    At Winona State University's Krueger Library, where Kendall Larson is an associate professor and Allison Quam is an assistant professor, informational and directional signs are an essential part of the interior space. Yet the ubiquitous sign and media saturation has challenged them to design and maintain effective signage that is conspicuous and…

  6. 75 FR 7597 - Change in Bank Control Notices; Acquisition of Shares of Bank or Bank Holding Companies

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-22

    ... Rakestraw, III, Somerset, Kentucky, individually; and The Rogers Family Immediate Family Control Group... M. Rogers, and John M. Rogers, all of Somerset, Kentucky, and Margaret Allison Rogers, Versailles... shares of Citizens ] National Bank of Somerset, both of Somerset, Kentucky. 2. Timothy Robert...

  7. Fuel cell/gas turbine integration

    SciTech Connect

    Knickerbocker, T.

    1995-10-19

    The Allison Engine Company`s very high efficiency fuel cell/advanced turbine power cycle program is discussed. The power cycle has the following advantages: high system efficiency potential, reduced emissions inherent to fuel cells, unmanned operation(no boiler) particularly suited for distributed power, and existing product line matches fuel cell operating environment. Cost effectiveness, estimates, and projections are given.

  8. Multi-Age Caregiving. Beginnings Workshop.

    ERIC Educational Resources Information Center

    Katz, Lilian G.; Allison, Jeanette; Clark, Marty; Bergman, Roberta; Gainer, Sue

    1998-01-01

    Presents four articles on the benefits of multi-age child care settings as being similar to a natural family setting. The workshop titles are: "The Benefits of the Mix" (Katz); "Dealing with Staff and Parent Concerns" (Allison); "Multi-Age at Riverfield Country Day School" (Clark); and "Making Multi-Age Groups Work" (Bergman and Gainer). (LBT)

  9. Gender Performativity in Physics: Affordances or Only Constraints?

    ERIC Educational Resources Information Center

    Danielsson, Anna T.; Lundin, Mattias

    2014-01-01

    In this forum we engage in a dialogue with Allison Gonsalves's paper '"Physics and the girly girl--there is a contradiction somewhere": Doctoral students' positioning around discourses of gender and competence in physics'. In her paper Gonsalves uses a sociocultural approach to examine women doctoral students'…

  10. Evidence of common signatures of selection in the genomes of West African cattle and the Yoruba human population

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In 1954, Allison found that the sickle-cell anemia mutation in the beta hemoglobin gene was highly prevalent in West African people because it is protective against malaria, so carriers would thrive and leave offspring in spite of the genetic disease. This is one of the earliest evidences of an envi...

  11. Boundless Opportunity

    ERIC Educational Resources Information Center

    Schaffhauser, Dian

    2009-01-01

    The easy availability of both web-based communication tools and classroom internet access presents teachers with myriad ways to engage their students in projects with kids all over the globe. Two teachers--English teacher Hagit Goldstein in Israel and Spanish teacher Allison Baugher in the US--were connecting students through an online classroom…

  12. Improving Cybersecurity Governance Through Data-Driven Decision-Making and Execution (Briefing Charts)

    DTIC Science & Technology

    2014-10-01

    Allison, G. T., & Zelikow, P. (1999). Essence of Decision: Explaining the Cuban Missile Crisis (2nd ed.) (Kindle Edition). New York: Longman. 17 Key...G. T., & Zelikow, P. (1999). Essence of Decision: Explaining the Cuban Missile Crisis (2nd ed.) (Kindle Edition). New York: Longman.

  13. A Statistical Model for Misreported Binary Outcomes in Clustered RCTs of Education Interventions

    ERIC Educational Resources Information Center

    Schochet, Peter Z.

    2013-01-01

    In randomized control trials (RCTs) of educational interventions, there is a growing literature on impact estimation methods to adjust for missing student outcome data using such methods as multiple imputation, the construction of nonresponse weights, casewise deletion, and maximum likelihood methods (see, for example, Allison, 2002; Graham, 2009;…

  14. 77 FR 42722 - Copper Valley Electric Association; Notice of Updated Environmental Analysis Preparation Schedule

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-20

    ... Energy Regulatory Commission Copper Valley Electric Association; Notice of Updated Environmental Analysis.... Applicant: Copper Valley Electric Association (Copper Valley). d. Name of Project: Allison Creek Project. e...)-825(r). g. Applicant Contact: Robert A. Wilkinson, CEO, Copper Valley Electric Association, P.O....

  15. 78 FR 61984 - Copper Valley Electric Association, Inc.; Notice of Application To Amend License and Accepted for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-09

    ... Energy Regulatory Commission Copper Valley Electric Association, Inc.; Notice of Application To Amend... 27, 2013. d. Applicant: Copper Valley Electric Association, Inc. e. Name of Project: Allison Creek.... Wilkinson, CEO, Copper Valley Electric Association, Inc., P.O. Box 45, Mile 187 Glenn Highway,...

  16. 3. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF MAIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF MAIN LOBBY. NOTE DETAIL OF MAHOGANY CARVING AND FIREPLACE MOLDINGS. STAIRWAY STILL SOLID AND WELL MAINTAINED. FLOORS PARQUET. GERMAN SILVER AND BRONZE CHANDELIER VALUED AT $10,000 WHEN PURCHASED IN 1914. Original in Collections of Marian College - James A. Allison Mansion, 3200 Coldspring Road, Indianapolis, Marion County, IN

  17. Hongkong Papers in Linguistics and Language Teaching, 12.

    ERIC Educational Resources Information Center

    Bruce, Nigel J., Ed.; Crewe, William J., Ed.

    1989-01-01

    Papers on linguistics and language teaching in this volume are the following: "The Revision Process in Academic Writing: From Pen and Paper to Word Processor" (Stephen Chadwick, Nigel Bruce); "Sentence Sequence and Coherence: In Search of Readers' Problems in Academic Discourse" (Desmond Allison); "Plural Marking in Hong Kong English" (Carol…

  18. Theories of State Analyzing the Policy Process,

    DTIC Science & Technology

    1973-11-01

    Bion; Leon Festinger, who studied "cognitive dissonance" Harold Kelley, Stanley Schachter and Dorwin Cartwright . 63 For application to policy-making in...Allison all have concrete proposals along these lines. The point all make is cogently summed up by that superb observer Charles Darwin , that no one

  19. The Transformative Power of Drama: Bringing Literature and Social Justice to Life

    ERIC Educational Resources Information Center

    Downey, Allison L.

    2005-01-01

    Allison L. Downey, a middle school English teacher, has used dramatic tableaux to help students think critically about literature as well as social and historic episodes. It is noted that students move to more abstract thinking, going beyond plot to an understanding of theme and metaphor.

  20. Didactique du francais langue seconde, dramatisation, et theatre (French Second Language Teaching, Dramatization, and Theater).

    ERIC Educational Resources Information Center

    Fancy, Alex

    1991-01-01

    Use of dramatics in French second language education is advocated with theoretical and practical arguments, and a four-stage approach to language acquisition through theater is proposed. The approach is based on activities of a bilingual theater troupe that offers Mount Allison University (New Brunswick) students an opportunity to participate in…

  1. HOME ENVIRONMENT AND CHILDHOOD ASTHMA IN A RURAL IOWA COUNTY

    EPA Science Inventory

    HOME ENVIRONMENT AND CHILDHOOD ASTHMA IN A RURAL IOWA COUNTY
    Erik R. Svendsen*?, Stephen J. Reynolds*?, James A. Merchant*, Allison L. Naleway*?, Ann M. Stromquist*, Peter S. Thorne*.
    *University of Iowa College of Public Health, Iowa City, IA ?Current: USEPA RTP, NC ?Curre...

  2. 4. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF FRENCH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Historic American Buildings Survey PHOTOCOPY CA. 1916 OF FRENCH ROOM LOOKING AT NORTH END OF ROOM. WALLS COVERED IN SILK WITH HAND- EMBROIDERED APPLIQUES. NOTE CARVINGS AND MOLDINGS IN WHITE GUM WOODWORK. FLOORS ARE PARQUET. Original in Collections of Marian College - James A. Allison Mansion, 3200 Coldspring Road, Indianapolis, Marion County, IN

  3. A Meta-Analysis of Peer-Mediated Interventions for Young Children with Autism Spectrum Disorders

    ERIC Educational Resources Information Center

    Zhang, Jie; Wheeler, John J.

    2011-01-01

    This meta-analysis investigated the efficacy of peer-mediated interventions for promoting social interactions among children from birth to eight years of age diagnosed with ASD. Forty-five single-subject design studies were analyzed and the effect sizes were calculated by the regression model developed by Allison and Gorman (1993). The overall…

  4. Improving Undergraduates' Argumentative Group Essay Writing through Self-Assessment

    ERIC Educational Resources Information Center

    Fung, Yong Mei; Mei, Hooi Chee

    2015-01-01

    When writing an argumentative essay, writers develop and evaluate arguments to embody, initiate, or simulate various kinds of interpersonal and textual interaction for reader consideration (Wu & Allison, 2003). This is quite challenging for English as a second language (ESL) learners. To improve the quality of their writing, students need to…

  5. Design and Testing of a Long Range Tele-Operated Missile System

    DTIC Science & Technology

    1998-01-01

    MRDEC Wing/Flap Assy. MRDEC PLGR 28 VDC* MRDEC Wing Deployment Mechanism MRDEC DC Power Supply Cable* MRDEC GS S/W Upgrade (AP/Nav) MRDEC Wiring ... Harnesses MRDEC DC Power Supply 208 VAC* MRDEC GS-PLGR Upgrade Rockwell Collins Engine Allison Inc. Remote SPIB Cable* MRDEC TM/FTS Test Box

  6. 48 CFR 1352.227-70 - Rights in data, assignment of copyright.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... of copyright. 1352.227-70 Section 1352.227-70 Federal Acquisition Regulations System DEPARTMENT OF....227-70 Rights in data, assignment of copyright. As prescribed in 48 CFR 1327.404-4(a), insert the following clause: Rights in Data, Assignment of Copyright (APR 2010) In accordance with 48 CFR...

  7. 48 CFR 1352.227-70 - Rights in data, assignment of copyright.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... of copyright. 1352.227-70 Section 1352.227-70 Federal Acquisition Regulations System DEPARTMENT OF....227-70 Rights in data, assignment of copyright. As prescribed in 48 CFR 1327.404-4(a), insert the following clause: Rights in Data, Assignment of Copyright (APR 2010) In accordance with 48 CFR...

  8. 48 CFR 1352.227-70 - Rights in data, assignment of copyright.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... of copyright. 1352.227-70 Section 1352.227-70 Federal Acquisition Regulations System DEPARTMENT OF....227-70 Rights in data, assignment of copyright. As prescribed in 48 CFR 1327.404-4(a), insert the following clause: Rights in Data, Assignment of Copyright (APR 2010) In accordance with 48 CFR...

  9. 48 CFR 1352.227-70 - Rights in data, assignment of copyright.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of copyright. 1352.227-70 Section 1352.227-70 Federal Acquisition Regulations System DEPARTMENT OF....227-70 Rights in data, assignment of copyright. As prescribed in 48 CFR 1327.404-4(a), insert the following clause: Rights in Data, Assignment of Copyright (APR 2010) In accordance with 48 CFR...

  10. 48 CFR 1352.227-70 - Rights in data, assignment of copyright.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... of copyright. 1352.227-70 Section 1352.227-70 Federal Acquisition Regulations System DEPARTMENT OF....227-70 Rights in data, assignment of copyright. As prescribed in 48 CFR 1327.404-4(a), insert the following clause: Rights in Data, Assignment of Copyright (APR 2010) In accordance with 48 CFR...

  11. 43 CFR 404.47 - How will a feasibility study be conducted under this program?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false How will a feasibility study be conducted... Studies § 404.47 How will a feasibility study be conducted under this program? Feasibility studies will be... feasibility study, including the Principles and Guidelines (incorporated by reference at § 404.4). You...

  12. 43 CFR 404.47 - How will a feasibility study be conducted under this program?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false How will a feasibility study be conducted... Studies § 404.47 How will a feasibility study be conducted under this program? Feasibility studies will be... feasibility study, including the Principles and Guidelines (incorporated by reference at § 404.4). You...

  13. MTR WING, TRA604. DETAILS OF LABORATORIES ALONG SOUTH WALL: TABLES, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    MTR WING, TRA-604. DETAILS OF LABORATORIES ALONG SOUTH WALL: TABLES, FUME HOODS, DESKS. BLAW-KNOX 3150-404-4, 7/1950. INL INDEX NO. 531-0604-00-098-100084, REV. 8. - Idaho National Engineering Laboratory, Test Reactor Area, Materials & Engineering Test Reactors, Scoville, Butte County, ID

  14. A novel mutation in exon 2 of FGB caused by c.221G>T (†) substitution, predicting the replacement of the native Arginine at position 74 with a Leucine (p.Arg74Leu (†) ) in a proband from a Kurdish family with dysfibrinogenaemia and familial venous and arterial thrombosis.

    PubMed

    Shlebak, Abdul A; Katsarou, Alexia D; Adams, George; Fernando, Fiona

    2017-02-01

    Dysfibrinogenaemias may present in either congenital or acquired form and are disorders of fibrinogen structure which may or may not be associated with abnormal function. More than 100 point mutations with single amino acid substitutions have been identified in over 400 families. These lead to defective DNA in the translated fibrinogen molecule. Such cases have improved our understanding of the fibrinogen-fibrin structure. Six members of a consanguineous family including a female proband, a female sibling, three male siblings and a daughter, with ages between 29 years and 53 years presented with early onset venous and premature arterial thromboembolic disease were investigated for a pro-thrombotic tendency associated with dysfibrinogenaemia. The family was investigated using standard coagulation assays and DNA sequencing of the genes encoding the FGA, FGB and FGG. All cases have dysfibrinogenaemia with a fibrinogen level 1.4 to 1.5 (1.9-4.3 g/L). Thrombophilia testing (including AT, PS & PC, F5 G1691A (FV Leiden)/F2 (prothombin G20210A) genotypes, homocysteine, antiphosphlipid antibody, paroxysmal nocturnal haemoglobinuria by flow cytometry and Janus Kinase-2 (exon 14)) were normal. PCR amplification and sequencing of exon 2 of FBG revealed a heterozygous mutation for a c.221G> T (†) substitution, predicting the replacement of the native Arginine at position 74 with a Leucine (p.Arg74Leu (†) ). In silico analysis of p.Arg74Leu strongly support pathogenicity. A novel mutation was identified in exon 2 of FGB caused by c.221G> T (†) substitution, predicting the replacement of Arginine at position 74 with a Leucine (p.Arg74Leu (†) ) in a proband from a Kurdish family with dysfibrinogenaemia and familial venous and arterial thrombosis.

  15. Theory and Application of the Wavelet Transform to Signal Processing

    DTIC Science & Technology

    1991-07-31

    discontinuity for the function g(t), providing g(t) is of bounded variation . Theorem 2: Let g(t) E L2 (R) and i(t) E L’(R) fL 2(R). Suppose g(t) is of... bounded variation in a neighborhood of to, then the wavelet transform of 9(t) with respect to the wavelet 0(t) has the property (i) 4og(s,to) --+ 0 as s...co. (28) Now consider 12. It is sufficient to consider the case where g(t) and O(t) are real valued. Because g(t) is of bounded variation in a

  16. Characterization of spermidine synthase and spermine synthase--The polyamine-synthetic enzymes that induce early flowering in Gentiana triflora.

    PubMed

    Imamura, Tomohiro; Fujita, Kohei; Tasaki, Keisuke; Higuchi, Atsumi; Takahashi, Hideyuki

    2015-08-07

    Polyamines are essential for several living processes in plants. However, regulatory mechanisms of polyamines in herbaceous perennial are almost unknown. Here, we identified homologs of two Arabidopsis polyamine-synthetic enzymes, spermidine synthase (SPDS) and spermine synthase (SPMS) denoted as GtSPDS and GtSPMS, from the gentian plant, Gentiana triflora. Our results showed that recombinant proteins of GtSPDS and GtSPMS possessed SPDS and SPMS activities, respectively. The expression levels of GtSPDS and GtSPMS increased transiently during vegetative to reproductive growth phase and overexpression of the genes hastened flowering, suggesting that these genes are involved in flowering induction in gentian plants.

  17. Phase Relations and Densities of Crustal Material Deeply Buried into the Mantle

    NASA Astrophysics Data System (ADS)

    Massonne, H.; Fockenberg, T.; Janitschke, M.

    2010-12-01

    To better approach the phase relations and corresponding densities of crustal material that is deeply buried into the mantle down to the transition zone, pseudosections were calculated for the P-T range 4-20 GPa and 600-1600°C in the system K2O-Na2O-CaO-FeO-MgO-Al2O3-SiO2-TiO2-H2O. These P-T pseudosections were obtained for various rock compositions typical for oceanic or continental crust among them are those of an S-type granite (G), a quartz-diorite (QD), a mid-ocean ridge basalt (MORB), a common pelite (P), an anatectic metapelite with restitic character (PR), and the averages of pelagic clay (APC) and upper continental crust (AUCC). For the calculation procedure, the PERPLE_X software package by Connolly and the thermodynamic data set by Holland and Powell, including corresponding solid-solution models, were used. To this data set, we have added thermodynamic data for stishovite, TiO2 with α-PbO2 structure, OH-topaz, phase egg, K-cymrite, Si-wadeite, K-hollandite, Na-hollandite, Na-pyroxene (NaPx), and Na-garnet (NaGt). The latter two phases with composition Na2MgSi5O12 were correspondingly included in four component solid-solution models for clinopyroxene and garnet. In addition, a new non-ideal solid-solution model for Na-K-hollandite was created. With these newly added thermodynamic data, the results of previous high-pressure experiments can be well reproduced. This concerns, for instance, the experiments in the system KAlSi3O8-NaAlSi3O8 by Yagi et al. on K-hollandite and its solid-solution towards Na-hollandite. Furthermore, the characteristics of MORB, studied by Okamoto and Maruyama in experiments up to 20 GPa, could be reproduced. In our study, NaPx and NaGt start to appear in the MORB composition above 7 GPa and reach maximum contents of 7 mol% of NaPx and 19 mol% of NaGt in pyroxene and garnet, respectively, at 14 GPa (1000-1400°C). At higher pressures, clinopyroxene widely disappears with only some nearly pure jadeite being left. Hollandite

  18. Observation of low- and high-energy Gamow-Teller phonon excitations in nuclei.

    PubMed

    Fujita, Y; Fujita, H; Adachi, T; Bai, C L; Algora, A; Berg, G P A; von Brentano, P; Colò, G; Csatlós, M; Deaven, J M; Estevez-Aguado, E; Fransen, C; De Frenne, D; Fujita, K; Ganioğlu, E; Guess, C J; Gulyás, J; Hatanaka, K; Hirota, K; Honma, M; Ishikawa, D; Jacobs, E; Krasznahorkay, A; Matsubara, H; Matsuyanagi, K; Meharchand, R; Molina, F; Muto, K; Nakanishi, K; Negret, A; Okamura, H; Ong, H J; Otsuka, T; Pietralla, N; Perdikakis, G; Popescu, L; Rubio, B; Sagawa, H; Sarriguren, P; Scholl, C; Shimbara, Y; Shimizu, Y; Susoy, G; Suzuki, T; Tameshige, Y; Tamii, A; Thies, J H; Uchida, M; Wakasa, T; Yosoi, M; Zegers, R G T; Zell, K O; Zenihiro, J

    2014-03-21

    Gamow-Teller (GT) transitions in atomic nuclei are sensitive to both nuclear shell structure and effective residual interactions. The nuclear GT excitations were studied for the mass number A = 42, 46, 50, and 54 "f-shell" nuclei in ((3)He, t) charge-exchange reactions. In the (42)Ca → (42)Sc reaction, most of the GT strength is concentrated in the lowest excited state at 0.6 MeV, suggesting the existence of a low-energy GT phonon excitation. As A increases, a high-energy GT phonon excitation develops in the 6-11 MeV region. In the (54)Fe → (54)Co reaction, the high-energy GT phonon excitation mainly carries the GT strength. The existence of these two GT phonon excitations are attributed to the 2 fermionic degrees of freedom in nuclei.

  19. Limited effect on NS3-NS4A protein cleavage after alanine substitutions within the immunodominant HLA-A2-restricted epitope of the hepatitis C virus genotype 3a non-structural 3/4A protease.

    PubMed

    Ahlén, Gustaf; Chen, Antony; Roe, Barbara; Falkeborn, Tina; Frelin, Lars; Hall, William W; Sällberg, Matti; Söderholm, Jonas

    2012-08-01

    It has been well established that immunological escape mutations within the hepatitis C virus genotype (gt) 1a non-structural (NS) 3/4A protease are partly prevented by a reduction in viral protease fitness. Surprisingly little is known about whether similar mutations affect proteases from other genotypes. In the present study, we assessed both the HLA-A2-restricted CTL response and gt3a NS3/4A protease fitness. Similar to gt1, the 1073-1081 epitope was immunodominant within the gt3a-specific HLA-A2-restricted CTL response, despite sequence similarity of only 56 % between the gt1a and gt3a genes. However, unlike the gt1a NS3/4A protease, all residues within the gt3a 1073-1081 epitope could be replaced sequentially by alanine while retaining protease activity, at least in part.

  20. Optical Probe for Semiconductor: Cooperative Research and Development Final Report, CRADA Number CRD-06-206

    SciTech Connect

    Sopori, B.

    2011-02-01

    This CRADA involves development of a new semiconductor characterization tool, Optical Probe, which can be commercialized by GT Solar. GT Solar will participate in the design and testing of this instrument that will be developed under an IPP project.

  1. Preclinical and Clinical Resistance Profile of EDP-239, a Novel Hepatitis C Virus NS5A Inhibitor

    PubMed Central

    Brasher, Bradley B.; Polemeropoulos, Alex; Rhodin, Michael H. J.; McAllister, Nicole; Wong, Kelly A.; Jones, Christopher T.; Jiang, Lijuan; Lin, Kai; Or, Yat Sun

    2016-01-01

    EDP-239, a potent and selective hepatitis C virus (HCV) nonstructural protein 5A (NS5A) inhibitor developed for the treatment of HCV infection, has been investigated in vitro and in vivo. This study sought to characterize genotypic changes in the HCV NS5A sequence of genotype 1 (GT1) replicons and to compare those changes to GT1 viral RNA mutations isolated from clinical trial patients. Resistance selection experiments in vitro using a subgenomic replicon identified resistance-associated mutations (RAMs) at GT1a NS5A amino acid positions 24, 28, 30, 31, and 93 that confer various degrees of resistance to EDP-239. Key RAMs were similarly identified in GT1b NS5A at amino acid positions 31 and 93. Mutations F36L in GT1a and A92V in GT1b do not confer resistance to EDP-239 individually but were found to enhance the resistance of GT1a K24R and GT1b Y93H. RAMs were identified in GT1 patients at baseline or after dosing with EDP-239 that were similar to those detected in vitro. Baseline RAMs identified at NS5A position 93 in GT1, or positions 28 or 30 in GT1a only, correlated with a reduced treatment response. RAMs at additional positions were also detected and may have contributed to reduced EDP-239 efficacy. The most common GT1a and GT1b RAMs found to persist up to weeks 12, 24, or 48 were those at NS5A positions 28, 30, 31, 58 (GT1a only), and 93. Those RAMs persisting at the highest frequencies up to weeks 24 or 48 were L31M and Q30H/R for GT1a and L31M and Y93H for GT1b. (This study has been registered at ClinicalTrials.gov under identifier NCT01856426.) PMID:27503644

  2. Variables Predicting Prospective Biology Teachers' Acceptance Perceptions Regarding Gene Technology

    ERIC Educational Resources Information Center

    Yilmaz, Mirac; Demirhan, Haydar

    2014-01-01

    The different opinions on products and applications of gene technology (GT) draw attention to the training and education activities related to GT. The purpose of this study is to review some variables predicting the acceptance perception regarding GT, and to investigate their changes at levels. The prospective teachers' subjective knowledge and…

  3. Hypolipidemic and Antioxidative Effects of Glossogyne tenuifolia in Hamsters Fed an Atherogenic Diet.

    PubMed

    Lee, Yi-Ning; Hsu, Guoo-Shyng Wang; Lin, Wan-Teng; Lu, Yi-Fa

    2016-05-01

    Glossogyne tenuifolia (GT) Cassini is a special herbal tea in the Penghu Islands, Taiwan, and has a long history of being used as an antipyretic, detoxifying, and anti-inflammatory remedy in folk medicine among local residents. The aim of this study was to investigate the effect of hot water extracts from GT on oxidative stress and lipid metabolism in animals. Five- to 6-week-old male Syrian hamsters were divided into four groups (n = 14) for different treatments, that is: control group (C), high-fat/cholesterol (HF) group, HF diet containing 0.5% (GT0.5) and 1.5% (GT1.5) GT extracts for 4 weeks. Hamsters fed with 0.5% GT powder as well as 1.5% GT powder exhibited reduced serum total cholesterol (TC), conjugated diene of low-density lipoprotein (LDL), and increased serum antioxidant capacity, but 1.5% GT powder was more potent at lowering serum LDL cholesterol and thiobarbituric acid reactive substance concentrations than 0.5% GT. GT extracts significantly lowered liver triacylglycerol (TG) concentration by diminishing activities of fatty acid synthase (FAS) and glucose-6-phosphate dehydrogenase (G-6-PDH). In addition, fecal excretion of cholesterol and bile acids were increased in GT extract groups. In conclusion, GT extracts increase the antioxidative capacity, decrease serum TC, inhibit the activities of FAS and G-6-PDH, and further reduce liver TG accumulation in hamster fed on atherogenic diets.

  4. Immunological comparison of sulfite oxidase

    SciTech Connect

    Pollock, V.; Barber, M.J. )

    1991-03-11

    Polyclonal antibodies (rabbit), elicited against FPLC-purified chicken and rat liver sulfite oxidase (SO), have been examined for inhibition and binding to purified chicken (C), rat (R), bovine (B), alligator (A) and shark (S) liver enzymes. Anti-CSO IgG cross-reacted with all five enzymes, with varying affinities, in the order CSO=ASO{gt}RSO{gt}BSO{gt}SSO. Anti-ROS IgG also cross-reacted with all five enzymes in the order RSO{gt}CSO=ASO{gt}BSO{gt}SSO. Anti-CSO IgG inhibited sulfite:cyt. c reductase (S:CR), sulfite:ferricyanide reductase (S:FR) and sulfite:dichlorophenolindophenol reductase (S:DR) activities of CSO to different extents (S:CR{gt}S:FR=S:DR). Similar differential inhibition was found for anti-ROS IgG and RSO S:CR, S:FR and S:DR activities. Anti-CSO IgG inhibited S:CR activities in the order CSO=ASO{much gt}SSO{gt}BSO. RSO was uninhibited. For anti-RSO IgG the inhibition order was RSO{gt}SSO{gt}BSO{gt}ASO. CSO was uninhibited. Anti-CSO and RSO IgGs partially inhibited Chlorella nitrate reductase (NR). Minor cross-reactivity was found for xanthine oxidase. Common antigenic determinants for all five SO's and NR are indicated.

  5. The effect of green tea and olive oil on the mutagenic activity of heterocyclic amines extracted from common food consumed in Saudi Arabia.

    PubMed

    Awney, Hala

    2011-05-01

    The effect of green tea (GT) and green tea with olive oil (GT+OL) as antioxidants on the formation and mutagenic activity of heterocyclic aromatic amines (HCAs) extracted from beef shawerma, grilled chicken and fried beef liver was examined. HCAs were extracted by blue rayon, analyzed as spiked and unspiked samples with high-performance liquid chromatography and its mutagenic response was assessed by Sallmonela typhimurium 100 in the Ames test. Surprisingly, GT and GT+OL augmented HCAs measured in beef shawerma and grilled chicken but total HCAs measured in GT+OL were less than GT treatment. Both treatments altered the HCA profile as imidazoquinoline type became the most abundant. In control and GT+OL fried beef liver no HCAs were detected, but Trp-P1 was detected in GT treatment. Generally, the mutagenic response of HCAs measured in GT+OL was less than GT in beef shawerma and grilled chicken. However, the mutagenic response of control and 2% GT+OL fried liver was negative. These data suggest that GT concentrations used in this study may induce free radical formation during the Millared reaction due to its pro-oxidative effect, which augmented the HCAs formed and its mutagenic response. In order to optimize both safety and quality of our diets, more need to be done to fully understand the risk of HCAs in food.

  6. The National Research Center on the Gifted and Talented Newsletter, 2000.

    ERIC Educational Resources Information Center

    Gubbins, E. Jean; Siegle, Del, Ed.

    2000-01-01

    These two newsletters from the National Research Center on the Gifted and Talented (NRC/GT) contain the following articles: (1) "NRC/GT: Professional Development--Not an Event" (E. Jean Gubbins), discusses NRC/GT research-based principles related to professional development and the importance of ongoing professional development; (2)…

  7. The Conflicts between Grounded Theory Requirements and Institutional Requirements for Scientific Research

    ERIC Educational Resources Information Center

    Luckerhoff, Jason; Guillemette, Francois

    2011-01-01

    The authors examined the conflicts between grounded theory (GT) requirements and institutional requirements for scientific research such as they were experienced by researchers and students. The overview of how GT was originally conceived served as background to the analysis of the problems GT users often faced when they submitted research…

  8. Molecular dissection of xylan biosynthesis during wood formation in poplar.

    PubMed

    Lee, Chanhui; Teng, Quincy; Zhong, Ruiqin; Ye, Zheng-Hua

    2011-07-01

    Xylan, being the second most abundant polysaccharide in dicot wood, is considered to be one of the factors contributing to wood biomass recalcitrance for biofuel production. To better utilize wood as biofuel feedstock, it is crucial to functionally characterize all the genes involved in xylan biosynthesis during wood formation. In this report, we investigated roles of poplar families GT43 and GT8 glycosyltransferases in xylan biosynthesis during wood formation. There exist seven GT43 genes in the genome of poplar (Populus trichocarpa), five of which, namely PtrGT43A, PtrGT43B, PtrGT43C, PtrGT43D, and PtrGT43E, were shown to be highly expressed in the developing wood and their encoded proteins were localized in the Golgi. Comprehensive genetic complementation coupled with chemical analyses demonstrated that overexpression of PtrGT43A/B/E but not PtrGT43C/D was able to rescue the xylan defects conferred by the Arabidopsis irx9 mutant, whereas overexpression of PtrGT43C/D but not PtrGT43A/B/E led to a complementation of the xylan defects in the Arabidopsis irx14 mutant. The essential roles of poplar GT43 members in xylan biosynthesis was further substantiated by RNAi down-regulation of GT43B in the hybrid poplar (Populus alba x tremula) leading to reductions in wall thickness and xylan content in wood, and an elevation in the abundance of the xylan reducing end sequence. Wood digestibility analysis revealed that cellulase digestion released more glucose from the wood of poplar GT43B RNAi lines than the control wood, indicating a decrease in wood biomass recalcitrance. Furthermore, RNAi down-regulation of another poplar wood-associated glycosyltransferase, PoGT8D, was shown to cause decreases in wall thickness and xylan content as well as in the abundance of the xylan reducing end sequence. Together, these findings demonstrate that the poplar GT43 members form two functionally non-redundant groups, namely PtrGT43A/B/E as functional orthologs of Arabidopsis IRX9 and PtrGT

  9. Understanding How Biomass Burning Impacts Climate Change

    ScienceCinema

    Aiken, Allison

    2016-10-12

    Biomass burning in Africa is creating a plume that spreads across the Atlantic Ocean all the way to Brazil. Allison Aiken, a research scientist at Los Alamos National Laboratory, collects data about the black carbon aerosols within this plume and their impact on the environment to help improve global climate modeling. A leader in energy science, Los Alamos develops climate models in support of the Laboratory’s mission to strengthen the nation’s energy security. Allison’s work is part of FIDO, a field operations team funded by the Energy Department’s Office of Science’s ARM Climate Research Facility.

  10. Dynamical Studies of Chemical Reactions Relevant to the Local Atmosphere of the Orbiting Space Shuttle.

    DTIC Science & Technology

    1998-02-01

    Cartechini, P. Casavecchia, E.H. van Kleef, G.G. Volpi, F.J. Aoiz , L. Banares, D.W. Schwenke, T.C. Allison, S.L. Mielke, and D.G. Truhlar Dynamics of...F.J. Aoiz , L. Banares, D.W. Schwenke, T.C. Allison, S.L. Mielke, and D.G. Truhlar The dynamics of the reaction CI + H 2 (D2 ) -> HCI(DCl) + H(D): A...respect to the previous study by P. Casavecchia, R.J. Buss, S.J. Sibener, and Y.T. Lee, J. Chem. Phys. 1980). 2. A.J. Alexander, F.J. Aoiz , M. Brouard

  11. Short-term green tea supplementation prevents recognition memory deficits and ameliorates hippocampal oxidative stress induced by different stroke models in rats.

    PubMed

    Altermann, Caroline Dalla Colletta; Souza, Mauren Assis; Schimidt, Helen L; Izaguirry, Aryele Pinto; Martins, Alexandre; Garcia, Alexandre; Santos, Francielli W; Mello-Carpes, Pâmela B

    2017-03-19

    This study investigated the effect of green tea (GT) on short and long term declarative memory and oxidative damage induced by transient ischemia-reperfusion (IR) and intracerebral hemorrhage (ICH) in rats. Male Wistar rats were divided into 8 groups of 10 according the stroke type induced: Sham IR, Sham IR+GT, IR, IR+GT, Sham ICH, Sham ICH+GT, ICH, ICH+GT. Supplementation with GT was initiated 10days before stroke surgery and continuous for 6days after (GT dose 400mg/kg). Short (STM) and long term memory (LTM) we evaluated with object recognition task (OR) and hippocampus were used to evaluate parameters related to oxidative stress (ROS, lipid peroxidation and total antioxidant capacity). The rats subjected to IR and ICH showed STM and LTM deficits and GT intervention prevented it in both stroke models. IR and ICH induced increase on ROS levels in hippocampus. ICH increased the lipid peroxidation in hippocampus and the GT supplementation avoided it. IR induced decrease on total antioxidant capacity and GT prevented it. These results reveal that GT supplementation presents a neuroprotective role, attenuates redox imbalance and might have a beneficial impact on cognitive function after stroke.

  12. Towards understanding the gliotoxin detoxification mechanism: in vivo thiomethylation protects yeast from gliotoxin cytotoxicity

    PubMed Central

    Smith, Elizabeth B.; Dolan, Stephen K.; Fitzpatrick, David A.; Doyle, Sean; Jones, Gary W.

    2016-01-01

    Gliotoxin (GT) is a mycotoxin produced by some species of ascomycete fungi including the opportunistic human pathogen Aspergillus fumigatus. In order to produce GT the host organism needs to have evolved a self-protection mechanism. GT contains a redox-cycling disulfide bridge that is important in mediating toxicity. Recently is has been demonstrated that A. fumigatus possesses a novel thiomethyltransferase protein called GtmA that has the ability to thiomethylate GT in vivo, which aids the organism in regulating GT biosynthesis. It has been suggested that thiomethylation of GT and similar sulfur-containing toxins may play a role in providing self-protection in host organisms. In this work we have engineered Saccharomyces cerevisiae, a GT-naïve organism, to express A. fumigatus GtmA. We demonstrate that GtmA can readily thiomethylate GT in yeast, which results in protection of the organism from exogenous GT. Our work has implications for understanding the evolution of GT self-protection mechanisms in organisms that are GT producers and non-producers. PMID:28357342

  13. Quantitative proteomics reveals the mechanism and consequence of gliotoxin-mediated dysregulation of the methionine cycle in Aspergillus niger.

    PubMed

    Manzanares-Miralles, Lara; Sarikaya-Bayram, Özlem; Smith, Elizabeth B; Dolan, Stephen K; Bayram, Özgür; Jones, Gary W; Doyle, Sean

    2016-01-10

    Gliotoxin (GT) is a redox-active metabolite, produced by Aspergillus fumigatus, which inhibits the growth of other fungi. Here we demonstrate how Aspergillus niger responds to GT exposure. Quantitative proteomics revealed that GT dysregulated the abundance of 378 proteins including those involved in methionine metabolism and induced de novo abundance of two S-adenosylmethionine (SAM)-dependent methyltransferases. Increased abundance of enzymes S-adenosylhomocysteinase (p=0.0018) required for homocysteine generation from S-adenosylhomocysteine (SAH), and spermidine synthase (p=0.0068), involved in the recycling of Met, was observed. Analysis of Met-related metabolites revealed significant increases in the levels of Met and adenosine, in correlation with proteomic data. Methyltransferase MT-II is responsible for bisthiobis(methylthio)gliotoxin (BmGT) formation, deletion of MT-II abolished BmGT formation and led to increased GT sensitivity in A. niger. Proteomic analysis also revealed that GT exposure also significantly (p<0.05) increased hydrolytic enzyme abundance, including glycoside hydrolases (n=22) and peptidases (n=16). We reveal that in an attempt to protect against the detrimental affects of GT, methyltransferase-mediated GT thiomethylation alters cellular pathways involving Met and SAM, with consequential dysregulation of hydrolytic enzyme abundance in A. niger. Thus, it provides new opportunities to exploit the response of GT-naïve fungi to GT.

  14. Influence of pH and diluent on the ion-pair solvent extraction of aromatic carboxylic acids using quaternary ammonium salts

    SciTech Connect

    Kawamura, K.; Takahashi, K.; Okuwaki, A.

    2006-07-01

    The influence of pH and diluent on the ion-pair solvent extraction of benzene polycarboxylic acids have been investigated for the separation of the coal oxidation products, which are formed by the treatment with alkaline solutions at high temperatures. Although the extent of the solvent extraction of benzoic acid (1BE) with a quaternary ammonium reagent (tri-n-octylmethylammonium chloride) into chloroform and benzene did not change at a very acidic and alkaline solutions, those of 1,2-benzenedicarboxylic acid (12BE) and trimellitic acid (124BE) somewhat decreased at very low pH and very high pH. The magnitudes of the equilibrium constants (K{sub ex}) of 1BE using a different diluent decreased in the order benzene {gt} carbontetrachloride {gt} 1,2-dichloroethane {gt} cyclohexane {gt} hexane {gt} chloroform {gt} 1-octanol and those of 12BE decreased in the order benzene {gt} cyclohexane {gt} carbontetrachloride {gt} hexane {gt} 1,2-dichloroethane {gt} chloroform. The inspection of the correlation between the values of K{sub ex} and several parameters of the diluent implies that the magnitude of K{sub ex} can be described by using the dielectric constant and the solubility parameter of diluent.

  15. Erosion Control of Scour during Construction. Report 5. Experimental Measurements of Refraction and Diffraction Downcoast of an Oblique Breakwater.

    DTIC Science & Technology

    1984-09-01

    Waterways Expt-riment Stat ion (WES iiter the gene .l supt Ivi- ,p I f Mssrs. H. B. Simmons , Chiet of the Ilydraul ics l.a irat j ry; . A !1,. 1! 1...IN THE FIELD .............. 603 [F (AT GT. A-R) GO TO 653 IF (A *GT. AT) Fr)I-1 . IF (A .GT. e3 R) FD1=1. IF (A .GT. A90) FD2 :1. IF (A .GT, AREF(<O...1RO./PI) FD2 -1. GO TO 35 633 IF (A .GT. AqEF(K])*190./Pl .AJD. A .LT. ABR) FD2 =1. S IF (A .GT. ABR .AND. A .LT. AT) FD1=-I. L

  16. Spatially-Resolved Characterization Techniques to Investigate Impact Damage in Ultra-High Performance Concretes

    DTIC Science & Technology

    2013-04-01

    ER D C/ G SL T R -1 3 -1 7 Spatially-Resolved Characterization Techniques to Investigate Impact Damage in Ultra- High Performance...Impact Damage in Ultra- High Performance Concretes Robert D. Moser, Paul G. Allison, and Mei Q. Chandler Geotechnical and Structures Laboratory US...Portland Cement concrete (OPC) and Ultra- High Performance Concretes (UHPCs) under high -strain impact and penetration loads at lower length scales

  17. Ambulatory Procedure Unit Coder Integration

    DTIC Science & Technology

    2005-04-01

    Michael N. Hendee, thank you for your countless hours of sea stories, mentorship, and taking care of a new Baylor Bear. To the many people who assisted...From the Patient Administration Department: LT Katrina A. Chenevert, LT Heather L. Taylor, and LTJG Blake A. Allison. For Coding Experts: CAPT Michael ...P. Dinneen , Lena E. Taylor, and Suzanne J. Leggieri. A special thanks to ENS John M. Gardner for proof reading and providing moral support throughout

  18. Secondary Power Systems Production Base Analysis Study

    DTIC Science & Technology

    1988-07-01

    Via Cueno 20 Torino 10152 Italy Engines: TG16, TG20 B-i General Electric Co. Marine & Industrial Engines & Service Div. 1 Neumann Way Cincinnati, OH...Engines & Service Div. 1 Neumann Way Cincinnati, OH 45215-6301 Engines: LM500, Frame 3 General Motors Allison Gas Turbine Division P.O. Box 420 Indianapolis...2089 Chemin du Pont de Rupe Toulouse France F-31019 Engines: Gevaudan, Dragan, Noelle , Saphir United Technologies Pratt & Whitney Aircraft Canada Box 10

  19. Fatigue and Fracture of Titanium Aluminides. Volume 2

    DTIC Science & Technology

    1990-02-01

    WRDC-TR-89-4145 Volume II FATIGUE AND FRACTURE OF TITANIUM ALUMINIDES M.L. Gambone V) Allison Gas Turbine Division fl General Motors Corporation RO...77 I1 TITLE (Include Securty Classficaton) Fat igue & Fracture of Titanium Aluminides 12. PERSONAL AUTHOR(S) M.L. Gambone 13& TYPE OF REPORT 13b. TIME...CODES 18. SUBJECT TERMS (Continue on reuerse it neceuar’y and identify by block numberi FIELD GROUP SUB GR. Metal matrix composites, titanium aluminide

  20. Hospital Ship Replacement

    DTIC Science & Technology

    2011-08-01

    designed to operate primarily when anchored to reduce the effects of roll. Quantum markets two separate zero speed active roll fin models for small ...ships. Feasibility of scaling fins to the size of the hospital ship requires validation. 3.12 Lifeboats and Liferafts The safety appliances designated ...for Innovation in Ship Design Technical Report Hospital Ship Replacement By Hannah Allison, Christopher Mehrvarzi, Rebecca Piks, Beau Lovdahl

  1. An Investigation of Aircraft Heaters. 16 - Determination of the Viscosity of Exhaust Gases from a Gasoline Engine

    DTIC Science & Technology

    1944-06-01

    measurements at low pressures, the mercury manometer was replaced with one of similar design but with a longer oolumn and using Allison* fluid, A serieB of...similar to those of the calibra- tion runs« RESULTS AETD DISCUSSION Preliminary runs.- The results of the preliminary runs with the mercury manometer indicated...on 008f were made at room temperature, at initial pressures of 2 to 7.5 inches of mercury (measured above atmospheric pressure with a mercury

  2. Advanced turbine systems program conceptual design and product development: Quarterly report, November 1993--January 1994

    SciTech Connect

    1995-01-01

    This report describes progress made in the advanced turbine systems program conceptual design and product development. The topics of the report include selection of the Allison GFATS, castcool technology development for industrial engines test plan and schedule, code development and background gathering phase for the ultra low NOx combustion technology task, active turbine clearance task, and water vapor/air mixture cooling of turbine vanes task.

  3. Aerobraking Teams

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Group and team photos of Langely's Aerobraking teams. These photo's were taken right after the 75 day aerobraking phase. People in the photographs include: Paul V. Tartabini, Mary Kae Lockwood, Richard W. Powell, Eric M. Queen, Bob Tolson, Alicia Dwyer, Jill Hanna, Michelle Munk, Zack Q. Chavis, dick Wilmoth, Naru Takashima, Ruth Amundsen, John Aguirre, Allison Roberts, Loreyna Young, Charles W. Davis, John Dec, Joe Gasbarre, Scott Striepe, Paul Escalera and G. M. Keating.

  4. Research and development of Proton-Exchange-Membrane (PEM) fuel cell system for transportation applications. Fuel cell infrastructure and commercialization study

    SciTech Connect

    1996-11-01

    This paper has been prepared in partial fulfillment of a subcontract from the Allison Division of General Motors under the terms of Allison`s contract with the U.S. Department of Energy (DE-AC02-90CH10435). The objective of this task (The Fuel Cell Infrastructure and Commercialization Study) is to describe and prepare preliminary evaluations of the processes which will be required to develop fuel cell engines for commercial and private vehicles. This report summarizes the work undertaken on this study. It addresses the availability of the infrastructure (services, energy supplies) and the benefits of creating public/private alliances to accelerate their commercialization. The Allison prime contract includes other tasks related to the research and development of advanced solid polymer fuel cell engines and preparation of a demonstration automotive vehicle. The commercialization process starts when there is sufficient understanding of a fuel cell engine`s technology and markets to initiate preparation of a business plan. The business plan will identify each major step in the design of fuel cell (or electrochemical) engines, evaluation of the markets, acquisition of manufacturing facilities, and the technical and financial resources which will be required. The process will end when one or more companies have successfully developed and produced fuel cell engines at a profit. This study addressed the status of the information which will be required to prepare business plans, develop the economic and market acceptance data, and to identify the mobility, energy and environment benefits of electrochemical or fuel cell engines. It provides the reader with information on the status of fuel cell or electrochemical engine development and their relative advantages over competitive propulsion systems. Recommendations and descriptions of additional technical and business evaluations that are to be developed in more detail in Phase II, are included.

  5. Orally Administered Bioadherent Sustained Release Microencapsulated Vaccines

    DTIC Science & Technology

    1996-09-01

    Bioadherent Sustained Release Microencapsulated Vaccines PRINCIPAL INVESTIGATOR: Dr. G. Duncan Hitchens, Anthony Giletto, Allison Rice-Ficht, Sunitha...Aug 96) 4. TITLE AND SUBTITLE 5. FUNDING NUMBERS Orally Administered Bioadherent Sustained Release Microencapsulated Vaccines DAMD17-95-C-5099 6... microencapsulated vaccine against staphylococcal enterotoxin A (SEA). The research is centered around using a known bioadhesive, vitelline protein B (vpB), to

  6. Latin America Report.

    DTIC Science & Technology

    1986-11-19

    quite firm and the mission of Interior Minister Ricardo Garcia is precisely to reaffirm the existing institutional system. The National Accord, which...Alberto Marquez Allison. Cruz Coke, who has not been notified about the indictment, said that for the time being he will not petition for the release of...within the opposition itself. The communists, as Gabriel Valdes said in his private letter, see the situation otherwise. They say that the DC is

  7. Emergent Management of Postpartum Hemorrhage for the General and Acute Care Surgeon

    DTIC Science & Technology

    2009-01-01

    who would try medical measures on their own, or call another obstetrical practitioner. Resuscitation Once significant postpartum hemorrhage has been...Research Clinical Obstetrics and Gynaecology 2002, 16(1):81-98. 12. Roberts WE: Emergent Obstetric Management of Postpar- tum Hemorrhage . Obstetrics and...Spring, MD, USA and 4Department of Obstetrics and Gynecology, Walter Reed Army Medical Center, Washington, DC, USA Email: Allison B Weisbrod

  8. GRACE and GEORGE: Autonomous Robots for the AAAI Robot Challenge

    DTIC Science & Technology

    2004-01-01

    GRACE and GEORGE: Autonomous Robots for the AAAI Robot Challenge Reid Simmons, Allison Bruce, Dani Goldberg, Adam Goode, Michael Montemerlo, Nicholas...2004 2. REPORT TYPE 3. DATES COVERED - 4. TITLE AND SUBTITLE GRACE and GEORGE: Autonomous Robots for the AAAI Robot Challenge 5a. CONTRACT...Simmons. “A Social Robot that Stands in Line.” Autonomous Robots , 12:3 pp.313-324, May 2002. [Ortony, 1988] A. Ortony, G. L. Clore, and A. Collins

  9. Proceedings of the 2012 Model-Based Systems Engineering Symposium, 27 - 28 November 2012, DSTO Edinburgh, South Australia

    DTIC Science & Technology

    2012-11-01

    DSTO) Wendy Butler (DSTO) Symposium Editor Michele Knight (DSTO) Social Coordinator Allison Lang (Aerospace Concepts) Administration Rebecca...Vehicles Division (Dr Ken Anderson) Doc Data Sht & Dist List Chief of Intelligence Surveillance and Reconnaissance Division (Dr Tony Lindsay ) Doc...KONEKSYS, Axel Reichwein 1 CD MEMKO, Shaunak Shroff 1 CD Participants - Defence ASE SQN, AOSG, Ming Chao 1 ASE SQN, AOSG, Scott Washusen 1 ASE SQN

  10. Federal Response to a Domestic Nuclear Attack (Counterproliferation Papers, Future Warfare Series, Number 46)

    DTIC Science & Technology

    2009-08-01

    a C-21A Learjet squadron at Scott Air Force Base, Illinois, tasked with responsibility for three geographically separated flying units. Federal...background for the four Supreme Court cases. Federal Response to a Domestic Nuclear Attack . . .15 Conditions of Necessity Though General Winfield Scott ...Response to a Domestic Nuclear Attack . . .27 Notes 1. Graham Allison , ―Preface,‖ Confronting the Specter of Nuclear Terrorism, The Annals of the

  11. Why the Bush Administration Invaded Iraq. Making Strategy after 9/11

    DTIC Science & Technology

    2008-01-01

    Disarming Iraq (New York: Pantheon, 2004), 3. 15. Ibid., 156. 16. See Graham Allison , Nuclear Terrorism: The Ultimate Preventable Catastrophe...2004): 51–72. 42. Scott Sagan, “The Perils of Proliferation: Organization Theory, Deterrence Theory, and the Spread of Nuclear Weapons...Condoleezza Rice and the Creation of the Bush Legacy (New York: St. Martin’s Press, 2007), 91. 47. Ibid., 98. 48. See Ivo H. Daalder and James M. Lindsay

  12. U.S. Nuclear Weapons: Changes in Policy and Force Structure

    DTIC Science & Technology

    2008-01-23

    2003. See, also, Butler, Amy. DTRA Director Pushes Conventionally- armed ICBMS. Defense Daily, January 26, 2004. p. 5. 96 Connolly, Allison ...U.S. Nuclear Forces. Natural Resources Defense Council. February 13, 2002. 125 Tyson, Anne Scott . New Push for Bunker-Buster Nuke. Christian Science...Sokolsky, Richard D. and Eugene B. Rumer. Nuclear Alarmists. Washington Post. March 15, 2002. p. 23. 150 Daalder, Ivo and James M. Lindsay . A New

  13. Acute Respiratory Distress Syndrome in Wartime Military Burns: Application of the Berlin Criteria

    DTIC Science & Technology

    2014-01-01

    Acute respiratory distress syndrome in wartime military burns: Application of the Berlin criteria Slava M. Belenkiy, MD, Allison R. Buel, DO, Jeremy...Andriy I. Batchinsky, MD, Leopoldo C. Cancio, MD, and Kevin K. Chung, MD, San Antonio, Texas BACKGROUND: Acute respiratory distress syndrome (ARDS...EVIDENCE: Epidemiologic/prognostic study, level III. KEY WORDS: Mechanical ventilation; adult respiratory distress syndrome ; the Berlin definition; combat

  14. Targeting the Vaccinia Virus L1 Protein to the Cell Surface Enhances Production of Neutralizing Antibodies

    DTIC Science & Technology

    2008-04-28

    vaccine can protect mice and non-humanprimates from lethal challengewith VACV ormon- keypox virus, respectively [21–23]. Fogg et al. demonstrated...23. 24] Fogg C, Lustig S, Whitbeck JC, Eisenberg RJ, Cohen GH, Moss B. Protective immunity to vaccinia virus induced by vaccination with multiple...Allison TJ, Fogg C, Moss B, Garboczi DN. The 1.51-Angstrom structure of the orthopoxvirus L1 protein, a target of potent neutralizing anti- bodies

  15. Environmental Design

    DTIC Science & Technology

    2015-04-13

    Allison~ Graham~ and Philip Zelikow. Essence of Decision: Explaining the Cuban Missile Crisis 2d ed. New York: Longm~~ 1996. Al-Azzawi, Souad...that caused the crisis in the first place are unaffected and in most cases, influencing the COG alone does not change 1 conditions. Influencing the...disrupt the Corps HQ directly and therefore severe all of the links from a key node? Arguably, missiles are not the only requirement for a maneuver

  16. Gamma-Insensitive Fast Neutron Detector with Spectral Source Identification Potential

    DTIC Science & Technology

    2011-03-01

    assistantship at ETH Zurich. Alexander Howard is a former CERN fellow and since 2001 a member of the GEANT4 collaboration, responsible for the...at Borders,” 2007 IEEE Nuclear Science Symposium Conference Record (2007). 10 S. Agostinelli et al., “ Geant4 – A Simulation Toolkit,” Nuclear...Instruments and Methods A 506, no. 3 (July 2003): 250-303: and J. Allison et al., “ Geant4 Developments and Applications,” IEEE Transactions on Nuclear

  17. The Relationship of Stress and Susceptibility to Infections in the Skin

    DTIC Science & Technology

    1990-08-31

    enclosed. a) Morhenn VB, Lee SW, lnicka M, Eugui EM : Activated human Langerhans cells express mRNA for interleukin-1 alpha and interleukin-1 beta and...produce these cytokines but do not secrete them. Submitted 1990. b) Lee SW, Morhenn VB, IUnicka M, Eugui EM , Allison AC: Autocrine stimulation of...papers are in press; titles below: a) Kilmer SL, Berman B, Morhenn VB: Eruptive seborrheic kerratoses in a young woman with acromegaly . J Am Acad

  18. On Disarmament: The Role of Conventional Arms Control in National Security Strategy

    DTIC Science & Technology

    1990-03-01

    both have their stable and predetermined place." Furthermore, Hannah Arendt insists that "since authority always demands obedience, it is commonly...Its Critics, ed. by Robert 0. Keohane, New York: Columbia University Press, 1986, p. 137. 5. Hannah Arendt , "What Is Authority?" in Between Past and...Talks." Defense News, February 13,1989, p. A3. Allison, Graham. "Success is Within Reach." The New York Times, February 19,1989, p. E19. Arendt , Hannah

  19. A Cross-Shore Model of Barrier Island Migration over a Compressible Substrate

    DTIC Science & Technology

    2010-01-01

    substrate Marine Geology 271 (2010) 1–16 ⁎ Corresponding author. Tel.: +1 251 694 3719; fax: +1 251 690 3464. E-mail address: Julie.D.Rosati...ScienceDirect Marine Geology j ourna l homepage: www.e lsev ie r.com/ locate /margeo Report Documentation Page Form ApprovedOMB No. 0704-0188 Public reporting...Egypt (Stanley and Warne, 1998), the Ganges–Brahmaputra Rivers, Bangladesh , India (Allison, 1998); and the Yangtze River, China (Xiqing, 1998

  20. Naked DNA Immunization of Prevention of Prostate Cancer in a Dunning Rat Prostate Tumor Model

    DTIC Science & Technology

    2006-06-01

    George Washington University Medical Center, Washington DC Central Laboratory for Immunology , St. Ivan Rilski Hospital, Sofia, Bulgaria Department of...author: Milcho Mincheff Head, Tumor Immunology Laboratory Department of Medicine The George Washington Medical Center 2300 Eye Street, N.W., Ross...regulatory role of CTLA-4. Immunity. 1995;3(5):541-547. 42. Egen JG, Allison JP. Cytotoxic T lymphocyte antigen-4 accumulation in the immunological synapse