Alpha Centauri at a Crossroads

NASA Astrophysics Data System (ADS)

Ayres, Thomas

2015-10-01

Nearby Alpha Centauri AB (G2V+K1V) contains the two best characterized solar-like dwarf stars, which also have the best studied multi-MK coronal X-ray activity cycles, extending back to the 1970's. Objective is to continue tracking the evolving multi-decadal high-energy narrative of Alpha Cen with semiannual X-ray pointings in Chandra Cycles 16-18, as the system reaches a coronal crossroads: solar twin A rising toward starspot cycle maximum, K-type companion B sinking into a minimum. HST/STIS UV spectra will support and leverage the X-ray measurements by probing chromospheric and subcoronal dynamics, with connection to the corona through the FUV Fe XII 1242 forbidden line. Only Chandra can resolve the AB X-ray pair as the Alpha Cen orbit also reaches a crossroads in 2016 (only 4 separation), and only HST/STIS can measure the bright Alpha Cen stars with sufficient UV spectral resultion and wavelength coherence. What's more, the recent validation of the STIS NDA,B,C long slits for echelle use now make feasible NUV E230H measurements (e.g., of key chromospheric tracers Mg II 2800 and Mg I 2852) which heretofore were not practical in a long-term program of this nature.

Alpha Centauri at a Crossroads

NASA Astrophysics Data System (ADS)

Ayres, Thomas

2016-10-01

Nearby Alpha Centauri AB (G2V+K1V) contains the two best characterized solar-like dwarf stars, which also have the best studied multi-MK coronal X-ray activity cycles, extending back to the 1970's. Objective is to continue tracking the evolving multi-decadal high-energy narrative of Alpha Cen with semiannual X-ray pointings in Chandra Cycles 16-18, as the system reaches a coronal crossroads: solar twin A rising toward starspot cycle maximum, K-type companion B sinking into a minimum. HST/STIS UV spectra will support and leverage the X-ray measurements by probing chromospheric and subcoronal dynamics, with connection to the corona through the FUV Fe XII 1242 forbidden line. Only Chandra can resolve the AB X-ray pair as the Alpha Cen orbit also reaches a crossroads in 2016 (only 4 separation), and only HST/STIS can measure the bright Alpha Cen stars with sufficient UV spectral resolution and wavelength coherence. What's more, the recent validation of the STIS NDA,B,C long slits for echelle use now make feasible NUV E230H measurements (e.g., of key chromospheric tracers Mg II 2800 and Mg I 2852) which heretofore were not practical in a long-term program of this nature.

A method to directly image exoplanets in multi-star systems such as Alpha-Centauri

NASA Astrophysics Data System (ADS)

Thomas, Sandrine J.; Belikov, Ruslan; Bendek, Eduardo

2015-09-01

Direct imaging of extra-solar planets is now a reality, especially with the deployment and commissioning of the first generation of specialized ground-based instruments such as the Gemini Planet Imager and SPHERE. These systems will allow detection of Jupiter-like planets 107 times fainter than their host star. Obtaining this contrast level and beyond requires the combination of a coronagraph to suppress light coming from the host star and a wavefront control system including a deformable mirror (DM) to remove residual starlight (speckles) created by the imperfections of telescope. However, all these current and future systems focus on detecting faint planets around single host stars, while several targets or planet candidates are located around nearby binary stars such as our neighboring star Alpha Centauri. Here, we present a method to simultaneously correct aberrations and diffraction of light coming from the target star as well as its companion star in order to reveal planets orbiting the target star. This method works even if the companion star is outside the control region of the DM (beyond its half-Nyquist frequency), by taking advantage of aliasing effects.

Project Blue: Optical Coronagraphic Imaging Search for Terrestrial-class Exoplanets in Alpha Centauri

NASA Astrophysics Data System (ADS)

Morse, Jon; Project Blue team

2018-01-01

Project Blue is a coronagraphic imaging space telescope mission designed to search for habitable worlds orbiting the nearest Sun-like stars in the Alpha Centauri system. With a 45-50 cm baseline primary mirror size, Project Blue will perform a reconnaissance of the habitable zones of Alpha Centauri A and B in blue light and one or two longer wavelength bands to determine the hue of any planets discovered. Light passing through the off-axis telescope feeds into a coronagraphic instrument that forms the heart of the mission. Various coronagraph designs are being considered, such as phase induced amplitude apodization (PIAA), vector vortex, etc. Differential orbital image processing techniques will be employed to analyze the data for faint planets embedded in the residual glare of the parent star. Project Blue will advance our knowledge about the presence or absence of terrestrial-class exoplanets in the habitable zones and measure the brightness of zodiacal dust around each star, which will aid future missions in planning their observational surveys of exoplanets. It also provides on-orbit demonstration of high-contrast coronagraphic imaging technologies and techniques that will be useful for planning and implementing future space missions by NASA and other space agencies. We present an overview of the science goals, mission concept and development schedule. As part of our cooperative agreement with NASA, the Project Blue team intends to make the data available in a publicly accessible archive.

DETECTABILITY OF EARTH-LIKE PLANETS IN CIRCUMSTELLAR HABITABLE ZONES OF BINARY STAR SYSTEMS WITH SUN-LIKE COMPONENTS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eggl, Siegfried; Pilat-Lohinger, Elke; Haghighipour, Nader, E-mail: siegfried.eggl@univie.ac.at

2013-02-20

Given the considerable percentage of stars that are members of binaries or stellar multiples in the solar neighborhood, it is expected that many of these binaries host planets, possibly even habitable ones. The discovery of a terrestrial planet in the {alpha} Centauri system supports this notion. Due to the potentially strong gravitational interaction that an Earth-like planet may experience in such systems, classical approaches to determining habitable zones (HZ), especially in close S-type binary systems, can be rather inaccurate. Recent progress in this field, however, allows us to identify regions around the star permitting permanent habitability. While the discovery ofmore » {alpha} Cen Bb has shown that terrestrial planets can be detected in solar-type binary stars using current observational facilities, it remains to be shown whether this is also the case for Earth analogs in HZs. We provide analytical expressions for the maximum and rms values of radial velocity and astrometric signals, as well as transit probabilities of terrestrial planets in such systems, showing that the dynamical interaction of the second star with the planet may indeed facilitate the planets' detection. As an example, we discuss the detectability of additional Earth-like planets in the averaged, extended, and permanent HZs around both stars of the {alpha} Centauri system.« less

Stability of Multi-Planet Systems in the Alpha Centauri System

NASA Technical Reports Server (NTRS)

Lissauer, Jack J.

2017-01-01

We evaluate the extent of the regions within the alpha Centauri AB star system where small planets are able to orbit for billion-year timescales (Quarles & Lissauer 2016, Astron. J. 151, 111), as well as how closely-spaced planetary orbits can be within those regions in which individual planets can survive. Although individual planets on low inclination, low eccentricity, orbits can survive throughout the habitable zones of both stars, perturbations from the companion star imply that the spacing of planets in multi-planet systems within the habitable zones of each star must be significantly larger than the spacing of similar multi-planet systems orbiting single stars in order to be long-lived. Because the binary companion induces a forced eccentricity upon the orbits of planets in orbit around either star, appropriately-aligned circumstellar orbits with small initial eccentricities are stable to slightly larger initial semimajor axes than are initially circular orbits. Initial eccentricities close to forced eccentricities can have a much larger affect on how closely planetary orbits can be spaced, and therefore on how many planets may remain in the habitable zones, although the required spacing remains significantly higher than for planets orbiting single stars.

The Centauri project: Manned interstellar travel

NASA Technical Reports Server (NTRS)

Ciesla, Thomas M.

1990-01-01

The development of antimatter engines for spacecraft propulsion will allow man to expand to the nearest stellar neighbors such as the Alpha Centuri system. Compared to chemically powered rockets like the Apollo mission class which would take 50,000 years to reach the Centauri system, antimatter propulsion would reduce one way trip time to 30 years or less. The challenges encountered by manned interstellar travel are formidable. The spacecraft must be a combination of sublight speed transportation system and a traveling microplanet serving an expanding population. As the population expands from the initial 100 people to approximately 300, the terraformed asteroid, enclosed by a man-made shell will allow for expansion over its surface in the fashion of a small terrestrial town. All aspects of human life - birth; death; physical, emotional, and educational needs; and government and law must be met by the structure, systems, and institutions on-board.

Planet Detectability in the Alpha Centauri System

NASA Astrophysics Data System (ADS)

Zhao, Lily; Fischer, Debra A.; Brewer, John; Giguere, Matt; Rojas-Ayala, Bárbara

2018-01-01

We use more than a decade of radial-velocity measurements for α {Cen} A, B, and Proxima Centauri from the High Accuracy Radial Velocity Planet Searcher, CTIO High Resolution Spectrograph, and the Ultraviolet and Visual Echelle Spectrograph to identify the M\\sin i and orbital periods of planets that could have been detected if they existed. At each point in a mass–period grid, we sample a simulated, Keplerian signal with the precision and cadence of existing data and assess the probability that the signal could have been produced by noise alone. Existing data places detection thresholds in the classically defined habitable zones at about M\\sin i of 53 {M}\\oplus for α {Cen} A, 8.4 {M}\\oplus for α {Cen} B, and 0.47 {M}\\oplus for Proxima Centauri. Additionally, we examine the impact of systematic errors, or “red noise” in the data. A comparison of white- and red-noise simulations highlights quasi-periodic variability in the radial velocities that may be caused by systematic errors, photospheric velocity signals, or planetary signals. For example, the red-noise simulations show a peak above white-noise simulations at the period of Proxima Centauri b. We also carry out a spectroscopic analysis of the chemical composition of the α {Centauri} stars. The stars have super-solar metallicity with ratios of C/O and Mg/Si that are similar to the Sun, suggesting that any small planets in the α {Cen} system may be compositionally similar to our terrestrial planets. Although the small projected separation of α {Cen} A and B currently hampers extreme-precision radial-velocity measurements, the angular separation is now increasing. By 2019, α {Cen} A and B will be ideal targets for renewed Doppler planet surveys.

Perturbations in the upper layers of α Centauri A

NASA Astrophysics Data System (ADS)

Brito, A.; Lopes, I.

2016-01-01

The emerging field of asteroseismology allows the direct study of stellar interiors with an incredibly high precision. We used a seismic parameter based on the phase shift as a diagnostic tool to infer the presence of a new layer of rapid variation in the external layers of the primary component of the stellar system Alpha Centauri AB. This layer is, apparently, a thin region where the acoustic modes suffer a strong scattering. Our tests indicate that this layer should be located at an acoustical depth of approximately 1400 s (0.939 R), which corresponds to a depth of 6% below the surface of the star. This is somehow unexpected since the internal structure of this sun-like star is predicted to be similar to the Sun.

The Ups and Downs of Alpha Centauri

NASA Astrophysics Data System (ADS)

Ayres, Thomas

2014-11-01

Nearby Alpha Centauri is destined for a pivotal chapter in human history, as first stop of future starfarers from Earth: 3x closer than the next nearest star; three very different objects to visit -- Alpha Cen A (G2V), B (K1V), and C (M6V); and B hosts an Earth-mass companion, albeit in a hot, lifeless orbit. For its part, Chandra has been keeping intent watch on the high-energy starspot cycles of AB, with semi-annual pointings over the past decade. Only HRC-I can separate AB as they plunge toward a close approach of 4" in 2016; and LETGS has countered that an abrupt 50x drop in XMM count rate of sun-like A in early 2005, ominously reported as the "darkening of the solar twin," simply is a soft sensitivity issue, not an unprecedented, inexplicable case of corona interrupta.

Density Determinations of the Coronae of Cool Stars Using a Newly Assigned Pair of Fe Xiv Lines in the Spectra of α Canis Minor, α Centauri, and the Sun [Density determinations of the coronae of cool stars using a newly assigned pair of Fe XIV lines in the spectra of alpha Canis Minor, alpha Centauri, and the Sun.

DOE PAGES

Beiersdorfer, P.; Hell, N.; Lepson, J. K.; ...

2015-12-02

We identified a previously unassigned pair of lines between 169 and 170 Å in the coronae of cool stars. Here, we attribute these lines to Fe xiv and show that their intensity ratio is sensitive to the electron density. Using observations taken with the Low Energy Transmission Grating Spectrometer of the Chandra X-ray Observatory we infer a density of log (n e/cm -3) = 10.2 ± 0.7 and 10.3 ± 0.8 from the newly identified line pair in the coronae of Procyon and α Cen A, respectively.

The Stellar-IRIS Connection: Four Years of FUV Measurements of Alpha Centauri by HST/STIS

NASA Astrophysics Data System (ADS)

Ayres, Thomas R.

2014-06-01

Since 2010 January, shortly after the miraculous repair of Hubble's Space Telescope Imaging Spectrograph (STIS) by SM4, the two sun-like stars of Alpha Centauri ("A" [G2V] and "B" [K1V]) have been recorded on a semi-annual basis utilizing STIS's far-ultraviolet (115-170 nm) medium resolution mode (about 8 km/s FWHM resolving power), jointly with an X-ray imaging study of AB by the Chandra Observatory. Both efforts are intended to assess the long-term behavior of high-energy (multimillion K) coronal, and subcoronal, processes on the two relatively low-activity solar-age dwarfs. In fact, the near-solar-twin Alpha Cen A has been mired in a coronal lull since 2005, originally recognized by XMM-Newton, and only recently has begun to climb out of the extended X-ray minimum. Meanwhile, the lower mass, lower luminosity, but coronally more active secondary has displayed a clear 8-year X-ray cycle, extending from the mid-1990's ROSAT era. The current study focuses on properties of the "transition zone" lines ( 100,000 K) of the Alpha Centauri stars, namely the bulk redshifts exhibited by the Si IV, C IV, and N V doublets; the multi-component nature of the hot-line profiles; behavior of the Fe XII 124 nm coronal forbidden line; and variability of the FUV fluxes relative to the higher-energy X-ray time series. These stellar measurements, with their high precision in wavelength and flux, complement the detailed high-spatial and high-temporal resolution spectral mapping of the solar corona and lower atmosphere being carried out by NASA's Interface Region Imaging Spectrograph (IRIS). [This work supported by GO grants 12758, 13060, and 13465 from Space Telescope Science Institute.

How Stable is a Light Sail Riding on a Laser Beam?

NASA Astrophysics Data System (ADS)

Kohler, Susanna

2017-03-01

The Breakthrough Starshot Initiative made headlines last year when the plan was first announced to send tiny spacecraft to our nearest stellar neighbors. But just how feasible is this initiative? A new study looks at just one aspect of this plan: whether we can propel the spacecraft successfully.Propelling a FleetThe Alpha Centauri star system, which consists of Alpha (left) and Beta (right) Centauri as well as Proxima Centauri (circled). [Skatebiker]The goal behind the Breakthrough Starshot Initiative is to build a fleet of tiny, gram-scale spacecraft to travel to the Alpha Centauri star system a systemin whicha planet was recently discovered around Proxima Centauri, the star nearest to us.To propel the spacecraft, the team plans to attach a reflective sail to each one. When a high-power laser beam is pointed at that sail from Earth, the impulse of the photons bouncing off the sail can acceleratethe lightweight spacecraft to a decent fraction of the speed of light, allowing it to reach the Alpha Centauri system within decades.Among the many potential engineering challenges forsuch a mission, one interesting one is examined in a recent study by Zachary Manchester and Avi Loeb of Harvard University: how do wekeep the spacecrafts light sail centered on the laser beam long enough to accelerate it?Beam profile (left) and corresponding potential function (right) for a laser beam made up of four Gaussians. With this configuration, the potential well pushes the spacecraft back to the center if it drifts toward the edges of the well. [Manchester Loeb 2017]The Search for StabilityManchester and Loeb arguethat any slight perturbations to the light sails position relative to the laser beam in the form of random disturbances, misalignments, or manufacturing imperfections could cause it to slide off the beam, preventing it from continuing toaccelerate. Ideally, the project would use a sail that could be passively stable: the sail wants to stay centered on the beam, rather than requiring active interference to keep it there.The scenario thats been proposed and studied in the past is that of a conical sail propelled by a Gaussian beam. But Manchester and Loeb perform analytic stability calculations to show that such a system will not, in fact, be stable if the beam gets knocked off the center of the sail, it will not be able to recover its centered position.Spheres on the GoSail position during beam-riding simulations for a spherical sail on the 4-Gaussian beam. Left: When the sail begins with a 5-cm offset from the center of the beam, it oscillates around the center but successfully remains bounded in the x-y plane (rather than drifting off the beam). Right: When noise is added to the beam, the sail oscillates more, but it still remains stable and bounded over several minutes of acceleration. [Manchester Loeb 2017]So if a conical sail wont work, what will instead? Manchester and Loeb propose an intriguing alternative: a light sail in the shape of a spherical shell around the spacecraft, propelled by a beam that is constructed from the sum of four Gaussians. This more complexconfiguration has the benefit that if the spacecraft is knocked off the center of the beam, it will experience a restoring force that pushes it back to the center. Thespherical shape of the sail means that it wont destabilize if its tilted.The authors perform a series of numerical simulations to test this configuration, demonstrating that it remains stable even when they introduce deliberate noise into the beam. The simulations show that thebeam can stay successfully centered on the spherical sail for at least several minutes sufficient for the spacecraft to be accelerated to a sizable fraction of the speed of light.So does this approach make Starshot feasible? It may be a step in the right direction, but challenges still remain. We can undoubtedly look forward to seeing further clever innovations as planning for this project continues!CitationZachary Manchester and Abraham Loeb 2017 ApJL 837 L20. doi:10.3847/2041-8213/aa619b

Project Dragonfly: A feasibility study of interstellar travel using laser-powered light sail propulsion

NASA Astrophysics Data System (ADS)

Perakis, Nikolaos; Schrenk, Lukas E.; Gutsmiedl, Johannes; Koop, Artur; Losekamm, Martin J.

2016-12-01

Light sail-based propulsion systems are a candidate technology for interplanetary and interstellar missions due to their flexibility and the fact that no fuel has to be carried along. In 2014, the Initiative for Interstellar Studies (i4is) hosted the Project Dragonfly Design Competition, which aimed at assessing the feasibility of sending an interstellar probe propelled by a laser-powered light sail to another star system. We analyzed and designed a mission to the Alpha Centauri system, with the objective to carry out science operations at the destination. Based on a comprehensive evaluation of currently available technologies and possible locations, we selected a lunar architecture for the laser system. It combines the advantages of surface- and space-based systems, as it requires no station keeping and suffers no atmospheric losses. We chose a graphene-based sandwich material for the light sail because of its low density. Deceleration of the spacecraft sufficient for science operations at the target system is achieved using both magnetic and electric sails. Applying these assumptions in a simulation leads to the conclusion that 250 kg of scientific payload can be sent to Alpha Centauri within the Project Dragonfly Design Competition's constraints of 100 year travel duration and 100 GW laser beam power. This is only sufficient to fulfill parts of the identified scientific objectives, and therefore renders the usefulness of such a mission questionable. A better sail material or higher laser power would improve the acceleration behavior, an increase in the mission time would allow for larger spacecraft masses.

Performance of advanced missions using fusion propulsion

NASA Technical Reports Server (NTRS)

Friedlander, Alan; Mcadams, Jim; Schulze, Norm

1989-01-01

A quantitive evaluation of the premise that nuclear fusion propulsion offers benefits as compared to other propulsion technologies for carrying out a program of advanced exploration of the solar system and beyond is presented. Using a simplified analytical model of trajectory performance, numerical results of mass requirements versus trip time are given for robotic missions beyond the solar system that include flyby and rendezvous with the Oort cloud of comets and with the star system Alpha Centauri. Round trip missions within the solar system, including robotic sample returns from the outer planet moons and multiple asteroid targets, and manned Mars exploration are also described.

The (Historical) Search for Planets Orbiting Proxima Centauri

NASA Astrophysics Data System (ADS)

Kohler, Susanna

2016-08-01

The European Southern Observatory (ESO) is widely expected to address the reportsofthe discovery of a planet orbiting our nearest stellar neighbor, Proxima Centauri, today. Due to its proximity 4.25 light-years away this red dwarf star has been a prime target for exoplanet searches throughout the last couple decades.Hubble image of Proxima Centauri, our nearest stellar neighbor. [ESA/Hubble]In anticipation of ESOspress conference this afternoon, lets take a look at someof the past work in the search for planetary companions around Proxima Centauri.The Early Years of Exploring Proxima CentauriProxima Centauri was discovered by astronomer Robert Innes in 1915. Studies of this star over the next eighty years primarily focused on better understanding its orbital motion (is it part of the Alpha Centauri star system?) and its flaring nature. But in the 1990s, after the detection of the first exoplanets, Proxima Centauri became a target for its potential to host planet-mass companions.Top: Images of Proxima Centauri on two different days from Hubbles FOS instrument. The bar across the center is an occulter that partially blocks the light from Proxima Centauri. Middle: Reconstructed images allowing a closer look at a moving feature identified by the authors as a possible companion. Bottom: diagram of the position of the planet candidate (box) relative to Proxima Centauri (star) in the two frames. [Schultz et al. 1998]1990s: A Possible Planet Detected With Hubble?In January 1998, a paper led by A.B. Schultz (STScI) reported the possible visual detection of a planetary companion to Proxima Centauri. Observations from Hubbles Faint Object Spectrograph, which was being used as a coronagraphic camera, revealed excess light that could be interpreted as a substellar object located ~0.5 AU from Proxima Centauri, a small separation that could imply either a short (~1 yr) period or a highly eccentric orbit.But follow-up observations led by David Golimowski (Johns Hopkins University) were unable to detect this proposed planet. These observations made by direct imaging with Hubbles Wide Field Planetary Camera 2 found no evidence of a companion located 0.121.1 AU from Proxima Centauri.In addition, an astrometric study led by G. Fritz Benedict (McDonald Observatory) the following year also didnt find any evidence for the proposed companion. Along with prior radial velocity measurements, the astrometry in this study ruled out all companions to Proxima Centauri with a mass of more than 0.8 Jupiter masses and periods between 1 and 1000 days.Increased Capabilities in Recent YearsWith increasing resolution and sensitivity of instruments, as well as better stellar modeling and increased noise-reduction strategies, we are now more likely than ever to be able to detect a planet orbiting Proxima Centauri. Therefore, our continued non-detections have been placing ever more stringent limits on the mass and orbital properties of a hypothetical companion.In 2014, as part of a long-term study of the solar neighborhood, a team led by John Lurie (University of Washington) published the results of a nearly 13-year campaign that used the Cerro Tololo Inter-American Observatory to obtain astrometric measurements for Proxima Centauri. This detailed study ruled out the possibility of Jupiter-mass companions at orbital periods of 212 years.Radial-velocity measurements of Proxima Centauri from a 2012 study using HARPS-TERRA. No promising signals of companions were found. [Anglada-Escud and Butler 2012]One of the most advanced instruments currently in the radial-velocity planet search is a spectrometer called the High Accuracy Radial velocity Planet Searcher (HARPS), operated by ESOin La Silla Observatory, Chile. In a study from 2012 led by Guillem Anglada-Escud (Carnegie Institution of Washington), the team described new data analysis algorithms being used with HARPS. The authors used Proxima Centauri as a test case, finding only a very marginal signal with a period of 5.6 days. The signals lack of significance led them to conclude that, unfortunately, no promising signals are yet detected on Proxima Cen.These studies among others throughout the last couple decades have placed strict limitations on the mass and orbit of a potential planetary companion to our nearest stellar neighbor. It will be interesting to see what ESO announces this afternoon, and how it fits into the context of these past studies of Proxima Centauri!CitationsA. B. Schultz et al 1998 AJ 115 345. doi:10.1086/300176David A. Golimowski and Daniel J. Schroeder 1998 AJ 116 440. doi:10.1086/300437G. Fritz Benedict et al 1999 AJ 118 1086. doi:10.1086/300975John C. Lurie et al 2014 AJ 148 91. doi:10.1088/0004-6256/148/5/91Guillem Anglada-Escud and R. Paul Butler 2012 ApJS 200 15. doi:10.1088/0067-0049/200/2/15

Discovery of a New Nearby Star

NASA Technical Reports Server (NTRS)

Teegarden, B. J.; Pravdo, S. H.; Covey, K.; Frazier, O.; Hawley, S. L.; Hicks, M.; Lawrence, K.; McGlynn, T.; Reid, I. N.; Shaklan, S. B.

2003-01-01

We report the discovery of a nearby star with a very large proper motion of 5.06 +/- 0.03 arcsec/yr. The star is called SO025300.5+165258 and referred to herein as HPMS (high proper motion star). The discovery came as a result of a search of the SkyMorph database, a sensitive and persistent survey that is well suited for finding stars with high proper motions. There are currently only 7 known stars with proper motions greater than 5 arcsec/yr. We have determined a preliminary value for the parallax of pi = 0.43 +/- 0.13 arcsec. If this value holds our new star ranks behind only the Alpha Centauri system (including Proxima Centauri) and Barnard's star in the list of our nearest stellar neighbours. The spectrum and measured tangential velocity indicate that HPMS is a main-sequence star with spectral type M6.5. However, if our distance measurement is correct, the HPMS is underluminous by 1.2 +/- 0.7 mag.

Five-minute P Modes Detected in Doppler Shift Measurement on Alpha Centauri

NASA Technical Reports Server (NTRS)

Fossat, E.; Grec, G.; Gelly, B.

1984-01-01

A spectrophotometer using the principle of optical resonance spectroscopy, designed for the goal of identifying radial and weakly non radial eigenmodes in the five minute range in the case of stars, is discussed. The conclusion of the first test of this new instrument was that if the observation can be photon noise limited (i.e., in total absence of any instrumental source of noise), the five-minute solar oscillation could still be detected by removing the Sun far enough for its magnitude to become zero or one. Such a situation is very closely represented by the observation of Alpha Centauri A, because it is a G2 V star, very similar to the Sun, with a mass of 1.1 in solar unit. Six nights were granted to this program on a 3.6m telescope, from 22 to 28 May 1983. Two and half nights provided over 20 hours of data of photometric quality good enough for analysis.

Techniques for High-contrast Imaging in Multi-star Systems. II. Multi-star Wavefront Control

NASA Astrophysics Data System (ADS)

Sirbu, D.; Thomas, S.; Belikov, R.; Bendek, E.

2017-11-01

Direct imaging of exoplanets represents a challenge for astronomical instrumentation due to the high-contrast ratio and small angular separation between the host star and the faint planet. Multi-star systems pose additional challenges for coronagraphic instruments due to the diffraction and aberration leakage caused by companion stars. Consequently, many scientifically valuable multi-star systems are excluded from direct imaging target lists for exoplanet surveys and characterization missions. Multi-star Wavefront Control (MSWC) is a technique that uses a coronagraphic instrument’s deformable mirror (DM) to create high-contrast regions in the focal plane in the presence of multiple stars. MSWC uses “non-redundant” modes on the DM to independently control speckles from each star in the dark zone. Our previous paper also introduced the Super-Nyquist wavefront control technique, which uses a diffraction grating to generate high-contrast regions beyond the Nyquist limit (nominal region correctable by the DM). These two techniques can be combined as MSWC-s to generate high-contrast regions for multi-star systems at wide (Super-Nyquist) angular separations, while MSWC-0 refers to close (Sub-Nyquist) angular separations. As a case study, a high-contrast wavefront control simulation that applies these techniques shows that the habitable region of the Alpha Centauri system can be imaged with a small aperture at 8× {10}-9 mean raw contrast in 10% broadband light in one-sided dark holes from 1.6-5.5 λ/D. Another case study using a larger 2.4 m aperture telescope such as the Wide-Field Infrared Survey Telescope uses these techniques to image the habitable zone of Alpha Centauri at 3.2× {10}-9 mean raw contrast in monochromatic light.

Observations of interstellar hydrogen and deuterium toward Alpha Centauri A

NASA Technical Reports Server (NTRS)

Landsman, W. B.; Henry, R. C.; Moos, H. W.; Linsky, J. L.

1984-01-01

A composite profile is presented of the Ly-alpha emission line of Alpha Cen A, obtained from 10 individual spectra with the high-resolution spectrograph aboard the International Ultraviolet Explorer (IUE) satellite. There is excellent overall agreement with two previous Copernicus observations. Interstellar deuterium is detected, and a lower limit is set on the deuterium to hydrogen ratio of nDI/nHI greater than 8 x 10 to the -6th. In addition, the deuterium bulk velocity appears blueshifted by 8 + or - 2 km/s with respect to interstellar hydrogen, suggesting a nonuniform medium along the line of sight.

Evolution of chromospheres and coronae in solar mass stars - A far-ultraviolet and soft X-ray comparison of Arcturus /K2 III/ and Alpha Centauri A /G2 V/

NASA Technical Reports Server (NTRS)

Ayres, T. R.; Simon, T.; Linsky, J. L.

1982-01-01

IUE far-UV and Einstein Observatory soft X-ray observations for the red giant Arcturus and the nearby yellow dwarf Alpha-Centauri A, which are archetypes of solar mass stars in different stages of evolution, are compared. Evidence is found for neither coronal soft X-ray emission from the red giant, at surface flux levels of only 0.0006 that detected previously for the yellow dwarf, nor C II and IV resonance line emission at surface flux levels of only 0.02 those of the yellow dwarf. The resonance line upper limits and previous detections of the C II intersystem UV multiplet 0.01 near 2325 A provide evidence for an Arcturus outer atmosphere that is geometrically extended, tenuous and cool. The red giant has, in addition, a prominent cool stellar wind. An extensive tabulation of line identifications, widths and fluxes for the IUE far-UV echelle spectra of the two stars is given, and two competing explanations for the Wilson-Bappu effect are discussed.

Antimatter rockets and interstellar propulsion

NASA Astrophysics Data System (ADS)

Cassenti, B. N.

1993-06-01

Propulsions systems based on the annihilation of matter can not only open up the solar system for human colonization but can reach the nearer stars. The nearest star to the sun, Alpha-Centauri C, is four light years distant (about 40 trillion km). Completing round trips to the nearer stars within the working lifetime of the crew will require velocities in excess of 20 percent of the speed of light. Of the rockets being considered today only rockets based on the annihilation of mass can complete these interstellar missions. This paper reviews the special theory of relativity and mass annihilation rockets and demonstrate the potential performance of antimatter rockets.

Sounds of a Star

NASA Astrophysics Data System (ADS)

2001-06-01

Acoustic Oscillations in Solar-Twin "Alpha Cen A" Observed from La Silla by Swiss Team Summary Sound waves running through a star can help astronomers reveal its inner properties. This particular branch of modern astrophysics is known as "asteroseismology" . In the case of our Sun, the brightest star in the sky, such waves have been observed since some time, and have greatly improved our knowledge about what is going on inside. However, because they are much fainter, it has turned out to be very difficult to detect similar waves in other stars. Nevertheless, tiny oscillations in a solar-twin star have now been unambiguously detected by Swiss astronomers François Bouchy and Fabien Carrier from the Geneva Observatory, using the CORALIE spectrometer on the Swiss 1.2-m Leonard Euler telescope at the ESO La Silla Observatory. This telescope is mostly used for discovering exoplanets (see ESO PR 07/01 ). The star Alpha Centauri A is the nearest star visible to the naked eye, at a distance of a little more than 4 light-years. The new measurements show that it pulsates with a 7-minute cycle, very similar to what is observed in the Sun . Asteroseismology for Sun-like stars is likely to become an important probe of stellar theory in the near future. The state-of-the-art HARPS spectrograph , to be mounted on the ESO 3.6-m telescope at La Silla, will be able to search for oscillations in stars that are 100 times fainter than those for which such demanding observations are possible with CORALIE. PR Photo 23a/01 : Oscillations in a solar-like star (schematic picture). PR Photo 23b/01 : Acoustic spectrum of Alpha Centauri A , as observed with CORALIE. Asteroseismology: listening to the stars ESO PR Photo 23a/01 ESO PR Photo 23a/01 [Preview - JPEG: 357 x 400 pix - 96k] [Normal - JPEG: 713 x 800 pix - 256k] [HiRes - JPEG: 2673 x 3000 pix - 2.1Mb Caption : PR Photo 23a/01 is a graphical representation of resonating acoustic waves in the interior of a solar-like star. Red and blue colours show element displacements in opposite directions. Geologists monitor how seismic waves generated by earthquakes propagate through the Earth, and thus learn about the inner structure of our planet. The same technique works for stars. The Sun, our nearest star and a typical middle-age member of its class, has been investigated in this way since the 1960's. With "solar seismology" , astronomers have been able to learn much about the inner parts of the star, and not only the outer layers normally visible to the telescopes. In the Sun, heat is bubbling up from the central regions where enormous amount of energy is created by nuclear reactions . In the so-called convective zone , the gas is virtually boiling, and hot gas-bubbles are rising with a speed that is close to that of sound. Much like you can hear when water starts to boil, the turbulent convection in the Sun creates noise . These sound waves then propagate through the solar interior and are reflected on the surface, making it oscillate. This "ringing" is well observed in the Sun, where the amplitude and frequency of the oscillations provide astronomers with plenty of information about the physical conditions in the solar interior. From the Sun to the stars There is every reason to believe that our Sun is a quite normal star of its type. Other stars that are similar to the Sun are therefore likely to pulsate in much the same way as the Sun. The search for such oscillations in other solar-like stars has, however, been a long and difficult one. The problem is simply that the pulsations are tiny, so very great precision is needed in the measurements. However, the last few years have seen considerable progress in asteroseismology, and François Bouchy and Fabien Carrier from the Geneva Observatory have now been able to detect unambiguous acoustic oscillations in the Solar-twin star, Alpha Centauri A. The bright and nearby star Alpha Centauri Alpha Centauri (Alpha Cen) [1] is the brightest star in the constellation Centaurus in the southern hemisphere. It is actually a double star, consisting of the solar twin Alpha Cen A and its fainter companion Alpha Cen B . A third star, Proxima Centauri , seems to be loosely connected with the binary. Proxima is, after the Sun, the nearest star we know now, only 4.3 light years away. However, Proxima is such a faint star that it can only be seen with a good telescope. Alpha Centauri A is one of the brightest stars on the southern sky, and the closest star observable with the naked eye. Being a near twin to the Sun and very nearby - in astronomical terms - made this star the ideal candidate in the search for tiny oscillations. The observational technique The observations of Alpha Cen A were conducted in May 2001 during five nights at the Swiss 1.2-m Leonard Euler telescope at the ESO La Silla Observatory. The CORALIE spectrograph at this telescope is well known for its very successful programme of detecting exoplanets, cf. PR 07/01. In searching for exoplanets close to nearby stars, the spectrograph identifies the tiny wobbling motion of the star, induced by the gravitational pull of the unseen planetary companion. The same technique was used for the investigation of oscillations in Alpha Cen A . The acoustic waves make the surface of the star periodically pulsate in and out, and the spectra of the stellar surface will show corresponding (very) small velocity shifts. The detected oscillations only move with speeds of up to 35 cm per second. This means that the perturbations on the stellar surface only amount to some 40 metres up and down on a star with a radius of 875,000 kilometres, see also the illustration ( PR Photo 23a/01 ). A very small change indeed, and not easy to detect with current astronomical instruments! Acoustic waves in Alpha Centauri A ESO PR Photo 23b/01 ESO PR Photo 23b/01 [Preview - JPEG: 369 x 400 pix - 22k] [Normal - JPEG: 738 x 800 pix - 45k] Caption : PR Photo 23b/01 represents the spectrum of acoustic modes , as observed in Alpha Cen A with the CORALIE spectrograph on the 1.2-m Swiss telescope at the ESO La Silla observatory. Several "eigenmodes" appear as high peaks in the frequency interval between 1.7 and 3 mHz; they correspond to oscillation periods in the range from 5 to 10 min. A very similar pattern is observed in the Sun. The data from five nights of observations were then searched to detect any changes of velocity and hence, oscillations. Astronomers use sophisticated mathematical methods for this kind of analysis, and normally present their results in terms of a "power spectrum" ( PR Photo 23b/01 ). It displays the "intensity" of oscillations at different frequencies, that is, of different periods; particularly high "peaks" indicate a "real" oscillation of that frequency. The comb of peaks visible between 2-3 mHz is the unambiguous and typical signature of solar-like oscillations . This frequency corresponds to a period of about 7 minutes, close to the well-known 5-minute oscillations of our Sun. This is in full agreement with expectations from theoretical models of the two stars. Continued detailed modeling of these new results will further improve the associated determination of the mass, radius, age, chemical composition and other properties of Alpha Cen A . This result is another proof of the excellent performance of the CORALIE spectrograph, providing extremely accurate measurements without the present investigation would have been impossible. Models of stellar interiors Our current understanding of stellar interiors is severely limited by lack of detailed and accurate observations of stars other than the Sun. In technical terms, for a complete description of the conditions inside a star, we need detailed knowledge of at least five stellar parameters (mass, age, initial content of helium and heavier elements, and a parameter describing the convection). However, in most cases, only two stellar properties can be measured directly (the temperature and the luminosity), so the models are necessarily quite uncertain (i.e., they are not well "constrained"). It is therefore imperative to enlarge the number of observables and this is possible with asteroseismology. Helioseismology has opened up the way. These observations severely constrain the possible models of the Sun's internal structure. But, depending on their mass and age, stars have very different internal structures, and may also harbour physical processes that are quite different from those in the Sun. Asteroseismological observations of stellar oscillations add crucial information that constrain the models of their inner structure, since the measured frequencies may be compared directly with those computed for the models. The observation of the full stellar disk allows to characterize certain (low degree) oscillation modes which penetrate deep inside the star and it is not necessary to resolve the stellar disk (as we can do for the Sun) in order to obtain useful seismological information. More stars to be observed Observations of bright solar-like stars are already planned with the CORALIE spectrograph. Even fainter stars can be observed with the HARPS spectrograph which will be installed on the 3.6-m telescope at La Silla Observatory at the end of 2002. It will be able to observe stars that are one hundred times fainter than those now reachable with CORALIE and with even better accuracy of the velocity measurements. While it will be mostly dedicated to the search of exoplanets, HARPS will be able to conduct an asteroseismological study of about 100 solar-like stars. More information The research reported in this Press Release is described in a scientific article ("P-mode observations on Alpha Cen A" by François Bouchy and Fabien Carrier) that has been accepted for publication as a Letter in the European journal "Astronomy & Astrophysics". Note [1]: Alpha Centauri was earlier known as Rigil Centauri , but that name is not much used because of the similarity with the name of the bright star Rigel in Orion. Alpha Centauri is one of the brightest stars in the sky (visual magnitude 0) and is a splendid view in the southern Milky Way, next to Beta Centauri . It was an object of worship on the Nile and the first visible emergence in the morning sky at the autumn equinox has been connected with the orientation of several temples in Northern and Southern Egypt from the fourth millenium B.C.

Beyond Kepler: Direct Imaging of Exoplanets

NASA Technical Reports Server (NTRS)

Belikov, Ruslan

2018-01-01

The exoplanets field has been revolutionizing astronomy over the past 20+ years and shows no signs of stopping. The next big wave of exoplanet science may come from direct imaging of exoplanets. Several (non-habitable) exoplanets have already been imaged from the ground and NASA is planning an instrument for its 2020s flagship mission (WFIRST) to directly image large exoplanets. One of the key goals of the field is the detection and characterization of "Earth 2.0", i.e. a rocky planet with an atmosphere capable of supporting life. This appears possible with several potential instruments in the late 2020s such as WFIRST with a starshade, Extremely Large Telescopes (ELTs) from the ground, or one of NASA possible flagship missions in the 2030s (HabEx or LUVOIR). Also, if an Earth-like planet exists around Alpha Centauri (A or B), it may be possible to directly image it in the next approx. 5 years with a small space mission such as the Alpha Centauri Exoplanet Satellite (ACESat). I will describe the current challenges and opportunities in this exciting field, as well as the work we are doing at the Exoplanet Technologies group to enable this exciting science.

NEAR: Low-mass Planets in α Cen with VISIR

NASA Astrophysics Data System (ADS)

Kasper, M.; Arsenault, R.; Käufl, H.-U.; Jakob, G.; Fuenteseca, E.; Riquelme, M.; Siebenmorgen, R.; Sterzik, M.; Zins, G.; Ageorges, N.; Gutruf, S.; Reutlinger, A.; Kampf, D.; Absil, O.; Carlomagno, B.; Guyon, O.; Klupar, P.; Mawet, D.; Ruane, G.; Karlsson, M.; Pantin, E.; Dohlen, K.

2017-09-01

ESO, in collaboration with the Breakthrough Initiatives, is working to modify the Very Large Telescope mid-IR imager (VISIR) to greatly enhance its ability to search for potentially habitable planets around both components of the binary Alpha Centauri, part of the closest stellar system to the Earth. Much of the funding for the NEAR (New Earths in the Alpha Cen Region) project is provided by the Breakthrough Initiatives, and ESO mostly provides staff and observing time. The concept combines adaptive optics using the deformable secondary mirror at Unit Telescope 4, a new annular groove phase mask (AGPM) coronagraph optimised for the most sensitive spectral bandpass in the N-band, and a novel internal chopper system for noise filtering based on a concept for longer wavelengths invented by the microwave pioneer Robert Dicke. The NEAR experiment is relevant to the mid-infrared METIS instrument on the Extremely Large Telescope, as the knowledge gained and proof of concept will be transferable.

Space telescope low scattered light camera - A model

NASA Technical Reports Server (NTRS)

Breckinridge, J. B.; Kuper, T. G.; Shack, R. V.

1982-01-01

A design approach for a camera to be used with the space telescope is given. Camera optics relay the system pupil onto an annular Gaussian ring apodizing mask to control scattered light. One and two dimensional models of ripple on the primary mirror were calculated. Scattered light calculations using ripple amplitudes between wavelength/20 wavelength/200 with spatial correlations of the ripple across the primary mirror between 0.2 and 2.0 centimeters indicate that the detection of an object a billion times fainter than a bright source in the field is possible. Detection of a Jovian type planet in orbit about alpha Centauri with a camera on the space telescope may be possible.

Long-term Stability of Tightly Packed Multi-planet Systems in Prograde, Coplanar, Circumstellar Orbits within the α Centauri AB System

NASA Astrophysics Data System (ADS)

Quarles, B.; Lissauer, Jack J.

2018-03-01

We perform long-term simulations, up to ten billion years, of closely spaced configurations of 2–6 planets, each as massive as the Earth, traveling on nested orbits about either stellar component in α Centauri AB. The innermost planet initially orbits at either the inner edge of its star’s empirical habitable zone (HZ) or the inner edge of its star’s conservative HZ. Although individual planets on low inclination, low eccentricity, orbits can survive throughout the HZs of both stars, perturbations from the companion star require that the minimum spacing of planets in multi-planet systems within the HZs of each star must be significantly larger than the spacing of similar multi-planet systems orbiting single stars in order to be long-lived. The binary companion induces a forced eccentricity upon the orbits of planets in orbit around either star. Planets on appropriately phased circumstellar orbits with initial eccentricities equal to their forced eccentricities can survive on more closely spaced orbits than those with initially circular orbits, although the required spacing remains higher than for planets orbiting single stars. A total of up to nine planets on nested prograde orbits can survive for the current age of the system within the empirical HZs of the two stars, with five of these orbiting α Centauri B and four orbiting α Centauri A.

Could photosynthesis function on Proxima Centauri b?

NASA Astrophysics Data System (ADS)

Ritchie, Raymond J.; Larkum, Anthony W. D.; Ribas, Ignasi

2018-04-01

Could oxygenic and/or anoxygenic photosynthesis exist on planet Proxima Centauri b? Proxima Centauri (spectral type - M5.5 V, 3050 K) is a red dwarf, whereas the Sun is type G2 V (5780 K). The light regimes on Earth and Proxima Centauri b are compared with estimates of the planet's suitability for Chlorophyll a (Chl a) and Chl d-based oxygenic photosynthesis and for bacteriochlorophyll (BChl)-based anoxygenic photosynthesis. Proxima Centauri b has low irradiance in the oxygenic photosynthesis range (400-749 nm: 64-132 µmol quanta m-2 s-1). Much larger amounts of light would be available for BChl-based anoxygenic photosynthesis (350-1100 nm: 724-1538 µmol quanta m-2 s-1). We estimated primary production under these light regimes. We used the oxygenic algae Synechocystis PCC6803, Prochlorothrix hollandica, Acaryochloris marina, Chlorella vulgaris, Rhodomonas sp. and Phaeodactylum tricornutum and the anoxygenic photosynthetic bacteria Rhodopseudomonas palustris (BChl a), Afifella marina (BChl a), Thermochromatium tepidum (BChl a), Chlorobaculum tepidum (BChl a + c) and Blastochloris viridis (BChl b) as representative photosynthetic organisms. Proxima Centauri b has only ~3% of the PAR (400-700 nm) of Earth irradiance, but we found that potential gross photosynthesis (P g) on Proxima Centauri b could be surprisingly high (oxygenic photosynthesis: earth ~0.8 gC m-2 h-1 Proxima Centauri b ~0.14 gC m-2 h-1). The proportion of PAR irradiance useable by oxygenic photosynthetic organisms (the sum of Blue + Red irradiance) is similar for the Earth and Proxima Centauri b. The oxygenic photic zone would be only ~10 m deep in water compared with ~200 m on Earth. The P g of an anoxic Earth (gC m-2 h-1) is ~0.34-0.59 (land) and could be as high as ~0.29-0.44 on Proxima Centauri b. 1 m of water does not affect oxygenic or anoxygenic photosynthesis on Earth, but on Proxima Centauri b oxygenic P g is reduced by ~50%. Effective elimination of near IR limits P g by photosynthetic bacteria (<10% of the surface value). The spectrum of Proxima Centauri b is unfavourable for anoxygenic aquatic photosynthesis. Nevertheless, a substantial aerobic or anaerobic ecology is possible on Proxima Centauri b. Protocols to recognize the biogenic signature of anoxygenic photosynthesis are needed.

IUE observations of interstellar hydrogen and deuterium toward Alpha Centauri B

NASA Technical Reports Server (NTRS)

Landsman, W. B.; Murthy, J.; Henry, R. C.; Moos, H. W.; Linsky, J. L.

1986-01-01

A high dispersion profile is presented of the Lyman-alpha emission toward Alpha Cen B as recorded in two images taken with the IUE spacecraft. The spectra were examined with a three-parameter Gaussian or five-parameter solar-type profile to derive the intrinsic background stellar emission. Voight absorption profiles were calculated for the intervening H I and D I gas. A uniform, thermally broadened medium was assumed, with the calculations being based on the free stellar parameters of density, velocity dispersion and the bulk velocity of H I, and the density of D I. The use of a small aperture is shown to have been effective in eliminating geocoronal and interplanetary diffuse Ly-alpha contamination. The H I absorption profile toward Alpha Cen B is found to be equivalent to that toward Alpha Cen A, indicating that the H I profiles derived are essentially independent of stellar emission. Less success, however, was attained in obtaining any definitive D I profile, although an asymmetry in the blue and red wings of the Lyman-alpha emissions did show the presence of absorption by interstellar deuterium and allow setting a lower limit of 0.00001 for the D I/H I ratio.

Line-profile and continuum variations of the contact binary SV Centauri

NASA Technical Reports Server (NTRS)

Rahe, J.; Drechsel, H.; Wargau, W.

1982-01-01

A total of five high and ten low dispersion UV spectra of the interacting contact binary SV Centauri obtained between 1979 and 1982 are analyzed. The low resolution observations cover the whole phase range, while a few selected phases were observed in high dispersion. The UV data were complemented with optical photometric and spectroscopic observations, in order to determine the tructure and absolute dimensions of the system. The profiles of prominent UV resonance and metastable lines undergo drastic changes with phase angle and time. Their overall appearance indicates relatively strong mass loss from the system, exhibiting pronounced variations of the stellar wind. The far UV continuum distribution suggests the presence of a luminous hot radiation source with maximum emission in the soft X-ray range, which is most apparently seen during the first quadrature phase, while it is weakest close to primary minimum. The case exchange and mass loss process as well as the evolutionary stage of SV Centauri are discussed.

Deriving stellar inclination of slow rotators using stellar activity signal

NASA Astrophysics Data System (ADS)

Dumusque, Xavier

2015-01-01

Stellar inclination is an important parameter for many astrophysical studies. In the context of exoplanets, this allows us to derive the true obliquity of a system if the projected stellar spin-planetary orbit angle can measured via the Rossiter-Mclaughlin effect. Although different techniques allow us to estimate stellar inclination for fast rotators, it becomes much more difficult when stars are rotating slower than 2-2.5 km.s-1. By using the new activity simulation SOAP 2.0 that can reproduce the photometric and spectroscopic variations induced by stellar activity, we are able to fit the activity variation of solar-type stars and derive their inclination. The case of the equator-on star HD189733 will be presented, as well as the case of Alpha Centauri B, which present an inclination of 45+9-19 degrees, implying that the earth-mass orbiting planet is not transiting if aligned with its host star. Other exemples will also demonstrate the power of the technique, that can infer a stellar inclination, even for slow rotators like Alpha Centauri B, that present a projected rotational velocity smaller than 1.15 km.s-1. In addition, the SOAP 2.0 simulation can be used to correct for the effect of activity when one major active region is dominating the RV signal. This could enhance the detection of small mass exoplanets orbiting slightly active stars.This project is funded by ETAEARTH (European Union Seventh Framework Programme (FP7/2007-2013) under Grant Agreement n. 313014), a transnational collaboration between European countries and the US (the Swiss Space Office, the Harvard Origin of Life Initiative, the Scottish Universities Physics Alliance, the University of Geneva, the Smithsonian Astrophysical Observatory, the Italian National Astrophysical Institute, the University of St. Andrews, Queens University Belfast, and the University of Edinburgh) setup to optimize the synergy between space-and ground-based data whose scientific potential for the characterization of extrasolar planets can only be fully exploited when analyzed together.

Planetary Formation and Dynamics in Binary Systems

NASA Astrophysics Data System (ADS)

Xie, J. W.

2013-01-01

As of today, over 500 exoplanets have been detected since the first exoplanet was discovered around a solar-like star in 1995. The planets in binaries could be common as stars are usually born in binary or multiple star systems. Although current observations show that the planet host rate in multiple star systems is around 17%, this fraction should be considered as a lower limit because of noticeable selection effects against binaries in planet searches. Most of the current known planet-bearing binary systems are S-types, meaning the companion star acts as a distant satellite, typically orbiting the inner star-planet system over 100 AU away. Nevertheless, there are four systems with a smaller separation of 20 AU, including the Gamma Cephei, GJ 86, HD 41004, and HD 196885. In addition to the planets in circumprimary (S-type) orbits discussed above, planets in circumbinary (P-type) orbits have been found in only two systems. In this thesis, we mainly study the planet formation in the S-type binary systems. In chapter 1, we first summarize current observational facts of exoplanets both in single-star and binary systems, then review the theoretical models of planet formation, with special attention to the application in binary systems. Perturbative effects from stellar companions render the planet formation process in binary systems even more complex than that in single-star systems. The perturbations from a binary companion can excite planetesimal orbits, and increase their mutual impact velocities to the values that might exceed their escape velocity or even the critical velocity for the onset of eroding collisions. The intermediate stage of the formation process---from planetesimals to planetary embryos---is thus the most problematic. In the following chapters, we investigate whether and how the planet formation goes through such a problematic stage. In chapter 2, we study the effects of gas dissipation on the planetesimals' mutual accretion. We find that in a dissipating gas disk, all the planetesimals eventually converge toward the same forced orbits regardless of their size, leading to the much lower impact velocities. This process progressively increases the net mass accretion and can even trigger the runaway growth for large planetesimals. In chapter 3, for the first time, we adopt a 3-dimensional approach to investigate the planetesimals' mutual accretion in binary systems. We find that the inclusion of a small inclination between the binary orbital plane and the circumstellar disk plane leads to the realization of the differential orbital phasing in 3-dimensional space. In such a case, impacts mainly occur between similar-sized bodies with the impact velocities being significantly reduced, and thus the planetesimal accretion is more favored. In chapter 4, we investigate the planet formation in a specific system, the habitable zone of Alpha Centauri B. For the first time, we develop a scaling method to estimate the planetesimal collisional timescale in binary systems. We find that the accretion-favorable conditions satisfied at 1˜2 AU from Alpha Centauri B after the first 10^5 years. However, the planetesimal accretion is significantly less efficient as compared to the single star case. Our results suggest that the formation of Earth-like planets through the accretion of km-sized planetesimals is possible in Alpha Centauri B, while the formation of gaseous giant planets is not favorable. In chapter 5, we outline a new concept, which we call the ``snowball'' growth mode. In this snowball phase, the isolated planetesimals move in the Keplerian orbits, and grow solely via the direct accretion of subcentimeter-sized dust entrained with the gas in the protoplanetary disk. Using a simplified model in which the planetesimals are progressively produced from the dust, we find that the snowball growth phase can be the dominant mode to transfer mass from the dust to planetesimals. The snowball growth mode could provide an alternative explanation for the turnover point in the size distribution of the present-day asteroid belt. For the specific case of close binaries such as Alpha Centauri, the snowball growth mode provides a safe way for the bodies to grow through the problematic range with a size of 1˜50 km. In chapter 6, we investigate the intermediate stages of the planet formation in highly inclined cases. We find that the gas drag plays a crucial role in the evolution of the planetesimals' semi-major axis, and the results can be generally divided into two categories, i.e., the Kozai-on regime and the Kozai-off regime. For both regimes, a robust outcome over a wide range of parameters is that, the planetesimals migrate/jump inwards and pile up, leading to a severely truncated and dense planetesimal disk around the primary. In this compact and dense disk, the collision rates are high but the relative velocities are low, providing conditions which are favorable for the planetesimal growth, and potentially allow for the subsequent formation of planets. Finally, we summarize this thesis in chapter 7. Many open questions still remain in current research field of planet formation in binary systems, and the current Kepler project provides an unprecedented opportunity for such researches. A comprehensive understanding of planets in binaries requires placing them in a bigger context to include the formation and evolution of stars and/or clusters.

PEERING INTO THE CORE OF A GLOBULAR CLUSTER

NASA Technical Reports Server (NTRS)

2002-01-01

Astronomers have used NASA's Hubble Space Telescope to peer into the center of a dense swarm of stars called Omega Centauri. Located some 17,000 light-years from Earth, Omega Centauri is a massive globular star cluster, containing several million stars swirling in locked orbits around a common center of gravity. The stars are packed so densely in the cluster's core that it is difficult for ground-based telescopes to make out individual stars. Hubble's high resolution is able to pick up where ground-based telescopes leave off, capturing distinct points of light from stars at the very center of the cluster. Omega Centauri is so large in our sky that only a small part of it fits within the field of view of the Wide Field and Planetary Camera 2 (WFPC2) on the Hubble Space Telescope. Yet even this tiny patch contains some 50,000 stars, all packed into a region only about 13 light-years wide. For comparison, a similarly sized region centered on the Sun would contain about a half dozen stars. The vast majority of stars in this Hubble image are faint, yellow-white dwarf stars similar to our Sun. The handful of bright yellow-orange stars are red giants that have begun to exhaust their nuclear fuel and have expanded to diameters about a hundred times that of the Sun. A number of faint blue stars are also visible in the image. These are in a brief phase of evolution between the dwarf stage and the red-giant stage, during which the surface temperature is high. The stars in Omega Centauri are all very old, about 12 billion years. Stars with a mass as high as that of our Sun have already completed their evolution and have faded away as white dwarfs, too faint to be seen even in the Hubble image. The stars in the core of Omega Centauri are so densely packed that occasionally one of them will actually collide with another one. Even in the dense center of Omega Centauri, stellar collisions will be infrequent. But the cluster is so old that many thousands of collisions have occurred. What happens when stars collide? These Hubble images were taken to help answer that question. When stars collide head-on, they probably just merge together and make one bigger star. But if the collision is a near miss, they may go into orbit around each other, forming a close binary star system. Searching for a needle in a haystack, scientists have found two binary star systems in these Hubble images that may have had such an origin. Both of them are close pairs in which once component is a white dwarf that pulls gas off of its companion. When the gas falls onto the surface of the white dwarf, it is heated to the point that it emits ultraviolet light. These unusual emissions enabled scientists to pinpoint these two faint stars among the myriad of other faint stars in the cluster. Omega Centauri is the most luminous and massive globular star cluster in the Milky Way. It is one of the few globular clusters that can be seen with the unaided eye. Named by Johann Bayer in 1603 as the 24th brightest object in the constellation Centaurus, it resembles a small cloud in the southern sky and might easily be mistaken for a comet. This Hubble WFPC2 image was taken on June 11, 1997 in ultraviolet, red, and H-alpha filters. The science team, led by Dr. Adrienne Cool of San Francisco State University includes Jennifer Carson, a former SFSU student who is now at UCLA, Charles Bailyn at Yale and Jonathan Grindlay at Harvard. These data are currently being used by Jeff Carlin and Daryl Haggard, two SFSU students, to look for optical counterparts of X-ray sources recently discovered with the Chandra Observatory. This image was produced by the Hubble Heritage Team (STScI/AURA). Credits: NASA and The Hubble Heritage Team (STScI/AURA) Acknowledgment: A. Cool (SFSU)

Centauri High School Teacher Honored as Colorado Outstanding Biology

Science.gov Websites

Teacher Centauri High School Teacher Honored as Colorado Outstanding Biology Teacher For more information contact: e:mail: Public Affairs Golden, Colo., May 2, 1997 -- Tracy Swedlund, biology teacher at Centauri High School in LaJara, was selected as Colorado's 1997 Outstanding Biology Teacher and will be

Terrestrial Planet Formation in Binary Star Systems

NASA Technical Reports Server (NTRS)

Lissauer, Jack J.; Quintana, Elisa V.; Chambers, John; Duncan, Martin J.; Adams, Fred

2003-01-01

Most stars reside in multiple star systems; however, virtually all models of planetary growth have assumed an isolated single star. Numerical simulations of the collapse of molecular cloud cores to form binary stars suggest that disks will form within such systems. Observations indirectly suggest disk material around one or both components within young binary star systems. If planets form at the right places within such circumstellar disks, they can remain in stable orbits within the binary star systems for eons. We are simulating the late stages of growth of terrestrial planets within binary star systems, using a new, ultrafast, symplectic integrator that we have developed for this purpose. We show that the late stages of terrestrial planet formation can indeed take place in a wide variety of binary systems and we have begun to delineate the range of parameter space for which this statement is true. Results of our initial simulations of planetary growth around each star in the alpha Centauri system and other 'wide' binary systems, as well as around both stars in very close binary systems, will be presented.

The Dynamo Clinical Trial

NASA Astrophysics Data System (ADS)

Ayres, Thomas R.

2016-04-01

The Dynamo Clinical Trial evaluates long-term stellar magnetic health through periodic X-ray examinations (by the Chandra Observatory). So far, there are only three subjects enrolled in the DTC: Alpha Centauri A (a solar-like G dwarf), Alpha Cen B (an early K dwarf, more active than the Sun), and Alpha Canis Majoris A (Procyon, a mid-F subgiant similar in activity to the Sun). Of these, Procyon is a new candidate, so it is too early to judge how it will fare. Of the other two, Alpha Cen B has responded well, with a steady magnetic heartbeat of about 8 years duration. The sickest of the bunch, Alpha Cen A, was in magnetic cardiac arrest during 2005-2010, but has begun responding to treatment in recent years, and seems to be successfully cycling again, perhaps achieving a new peak of magnetic health in the 2016 time frame. If this is the case, it has been 20 years since A's last healthful peak, significantly longer than the middle-aged Sun's 11-year magnetic heartbeat, but perhaps in line with Alpha Cen A's more senescent state (in terms of "relative evolutionary age," apparently an important driver of activity). (By the way, don't miss the exciting movie of the Alpha Cen stars' 20-year X-ray dance.)

Spinning Like a Blue Straggler: The Population of Fast Rotating Blue Straggler Stars in ω Centauri

NASA Astrophysics Data System (ADS)

Mucciarelli, A.; Lovisi, L.; Ferraro, F. R.; Dalessandro, E.; Lanzoni, B.; Monaco, L.

2014-12-01

By using high-resolution spectra acquired with FLAMES-GIRAFFE at the ESO/VLT, we measured the radial and rotational velocities for 110 blue straggler stars (BSSs) in ω Centauri, the globular cluster-like stellar system harboring the largest known BSS population. According to their radial velocities, 109 BSSs are members of the system. The rotational velocity distribution is very broad, with the bulk of BSSs spinning at less than ~40 km s-1 (in agreement with the majority of such stars observed in other globular clusters) and a long tail reaching ~200 km s-1. About 40% of the sample has ve sin i > 40 km s-1 and about 20% has ve sin i > 70 km s-1. Such a large fraction is very similar to the percentage of fast rotating BSSs observed in M4. Thus, ω Centauri is the second stellar cluster, beyond M4, with a surprisingly high population of fast spinning BSSs. We found a hint of radial behavior for a fraction of fast rotating BSSs, with a mild peak within one core radius, and a possible rise in the external regions (beyond four core radii). This may suggest that recent formation episodes of mass transfer BSSs occurred preferentially in the outskirts of ω Centauri, or that braking mechanisms able to slow down these stars are least efficient in the lowest density environments. Based on observations collected at the ESO-VLT under the programs 077.D-0696(A), 081.D-0356(A), and 089.D-0298(A).

Light Curve and Analysis of the Eclipsing Binary BF Centauri

NASA Astrophysics Data System (ADS)

Morris, M. A.; Wolf, G. W.

2003-12-01

The eclipsing binary star BF Centauri was observed photometrically by GWW in the uvby filter system from Mt. John Observatory in New Zealand during 1982, 1989 and 1998. It was also observed spectroscopically at 10 A/mm by W. A. Lawson in 1993 at Mt. Stromlo in Australia to obtain a radial velocity solution. The combined light curves and spectroscopic results have been analyzed using the 1998 version of Robert Wilson's WD light-curve programs. A consistent model for the system will be presented. This analysis was done as a part of a senior research project by MAM, who would like to acknowledge financial support from the Missouri Space Grant Consortium.

Fusion energy for space missions in the 21st Century

NASA Technical Reports Server (NTRS)

Schulze, Norman R.

1991-01-01

Future space missions were hypothesized and analyzed and the energy source for their accomplishment investigated. The mission included manned Mars, scientific outposts to and robotic sample return missions from the outer planets and asteroids, as well as fly-by and rendezvous mission with the Oort Cloud and the nearest star, Alpha Centauri. Space system parametric requirements and operational features were established. The energy means for accomplishing the High Energy Space Mission were investigated. Potential energy options which could provide the propulsion and electric power system and operational requirements were reviewed and evaluated. Fusion energy was considered to be the preferred option and was analyzed in depth. Candidate fusion fuels were evaluated based upon the energy output and neutron flux. Reactors exhibiting a highly efficient use of magnetic fields for space use while at the same time offering efficient coupling to an exhaust propellant or to a direct energy convertor for efficient electrical production were examined. Near term approaches were identified.

Cosmic Rays near Proxima Centauri b

NASA Astrophysics Data System (ADS)

Sadovski, A. M.; Struminsky, A. B.; Belov, A.

2018-05-01

The discovery of a terrestrial planet orbiting Proxima Centauri has led to a lot of papers discussing the possible conditions on this planet. Since the main factors determining space weather in the Solar System are the solar wind and cosmic rays (CRs), it seems important to understand what the parameters of the stellar wind, Galactic and stellar CRs near exoplanets are. Based on the available data, we present our estimates of the stellar wind velocity and density, the possible CR fluxes and fluences near Proxima b. We have found that there are virtually no Galactic CRs near the orbit of Proxima b up to particle energies 1 TeV due to their modulation by the stellar wind. Nevertheless, more powerful and frequent flares on Proxima Centauri than those on the Sun can accelerate particles to maximum energies 3150 αβ GeV ( α, β < 1). Therefore, the intensity of stellar CRs in the astrosphere may turn out to be comparable to the intensity of low-energy CRs in the heliosphere.

Direct imaging of exoplanets around multiple star systems

NASA Astrophysics Data System (ADS)

Thomas, Sandrine

2015-01-01

Direct imaging of extra-solar planets is now a reality, especially with the deployment and commissioning of the first generation of specialized ground-based instruments such as the Gemini Planet Imager and SPHERE. These systems will allow detection of Jupiter-like planets 10^7 times fainter than their host star. Obtaining this contrast level and beyond requires the combination of a coronagraph to suppress light coming from the host star and a wavefront control system including a deformable mirror (DM) to remove residual starlight (speckles) created by the imperfections of telescope. However, all these current and future systems focus on detecting faint planets around a single host star or unresolved binaries/multiples, while several targets or planet candidates are located around nearby binary stars such as our neighboring star Alpha Centauri.Here, we present a method to simultaneously correct aberrations and diffraction of light coming from the target star as well as its companion star in order to reveal planets orbiting the target star. This method works even if the companion star is outside the control region of the DM (beyond its half-Nyquist frequency), by taking advantage of aliasing effects.

Analysis of photometric light curves solution for massive contact OB binary stars. LY Aurigae, BH Centauri, SV Centauri

NASA Astrophysics Data System (ADS)

Avvakumova, E. A.

2010-01-01

We searched for signs of the presence of circumstellar gaseous matter in photometric data for massive contact early-type binaries by analyzing residual curves (the dependence of the difference between the observed and theoretical brightness variations on the orbital-period phase) for three such stars. The residual curves make it possible to estimate the influence of gas in the common envelope on the observed light curves for different phase intervals and to qualitatively describe the character of the distortion of the light from the system’s components. Changes of the residual curves from filter to filter indicate varying conditions in the circumstellar matter. Changes of the residual curves from one observation epoch to another indicate varying conditions in the circumstellar matter. We compared the residual curves obtained for different photometric bands and epochs via a correlation analysis. The distortion of light from the components of LY Aurigae in the ultraviolet differs from that in the visual. The distortion of light from the components of SV Centauri is appreciable, but not selective, and does not vary in time, while the distortion of light from BH Centauri possesses a strong selective component. A comparison of the radii computed for the components of BH Centauri and SV Centauri shows that the gas distribution near these binaries varies in time.

View of Southern Cross, Alpha and Beta Centauri

NASA Image and Video Library

1996-03-18

STS075-351-022 (22 Feb.- 9 March 1996) --- The space shuttle Columbia's vertical stabilizer appears to point to the four stars of the Southern Cross. The scene was captured with a 35mm camera just prior to a sunrise. The seven member crew was launched aboard the space shuttle Columbia on Feb. 22, 1996, and landed on March 9, 1996. Crew members were Andrew M. Allen, mission commander; Scott J. Horowitz, pilot; Franklin R. Chang-Diaz, payload commander; and Maurizio Cheli, European Space Agency (ESA); Jeffrey A. Hoffman and Claude Nicollier, ESA, all mission specialists; along with payload specialist Umberto Guidoni of the Italian Space Agency (ASI).

An IUE's eye view of cool-star outer atmospheres

NASA Technical Reports Server (NTRS)

Ayres, T. R.

1981-01-01

Three topics are discussed which together demonstrate the power of the IUE to probe the occurrences of chromospheres and coronas in the cool half of the HR diagram. These are: (1) the complementary low dispersion and echelle observing modes; (2) Mg II h and k: chromospheric cooling and width luminosity correlation; and (3) empirical correlations among chromospheric, transition region, and coronal emission. The spectra of alpha Centauri (G2 V + K1 V) and Capella (G6 III + F9 III) are compared with that of the Sun and recent low dispersion surveys of cool star emission in the 1150 A to 2000 A short wavelength region are summarized.

Omega Centauri Looks Radiant in Infrared

NASA Image and Video Library

2008-04-10

A cluster brimming with millions of stars glistens like an iridescent opal in this image from NASA Spitzer Space Telescope. Called Omega Centauri, the sparkling orb of stars is like a miniature galaxy.

Searching for faint comoving companions to the α Centauri system in the VVV survey infrared images

NASA Astrophysics Data System (ADS)

Beamín, J. C.; Minniti, D.; Pullen, J. B.; Ivanov, V. D.; Bendek, E.; Bayo, A.; Gromadzki, M.; Kurtev, R.; Lucas, P. W.; Butler, R. P.

2017-12-01

The VVV survey has observed the southern disc of the Milky Way in the near-infrared, covering 240 deg2 in the ZYJHKs filters. We search the VVV survey images in a ∼19 deg2 field around α Centauri, the nearest stellar system to the Sun, to look for possible overlooked companions that the baseline in time of VVV would be able to uncover. The photometric depth of our search reaches Y ∼ 19.3 mag, J ∼ 19 mag, and Ks ∼ 17 mag. This search has yielded no new companions in α Centauri system, setting an upper mass limit for any unseen companion well into the brown dwarf/planetary mass regime. The apparent magnitude limits were turned into effective temperature limits, and the presence of companion objects with effective temperatures warmer than 325 K can be ruled out using different state-of-the-art atmospheric models. These limits were transformed into mass limits using evolutionary models, companions with masses above 11MJup were discarded, extending the constraints recently provided in the literature up to projected distances of d < 7000 au from α Cen AB and ∼1 200 au from Proxima. In the next few years, the VVV extended survey (VVVX) will allow us to extend the search and place similar limits on brown dwarfs/planetary companions to α Cen AB for separations up to 20 000 au.

Chandra X-ray Time-Domain Study of Alpha Centauri AB, Procyon, and their Environs

NASA Astrophysics Data System (ADS)

Ayres, Thomas R.

2018-06-01

For more than a decade, Chandra X-ray Observatory has been monitoring the central AB binary (G2V+K1V) of the α Centauri triple system with semi-annual pointings, using the High-Resolution Camera. This study has been extended in recent years to the mid-F subgiant, Procyon. The main objective is to follow the coronal (T~1MK) activity variations of the three stars, analogous to the Sun's 11-year sunspot cycle. Tentative periods of 20 yr and 8 yr have been deduced for α Cen A and B, respectively; but so far Procyon has shown only a slow, very modest decline in count rate, which could well reflect a slight instrumental degradation rather than intrinsic behavior. The negligible high-energy variability of Procyon sits in stark contrast to the dramatic factor of several to ten changes in the X-ray luminosities of α Cen AB and the Sun over their respective cycles. Further, although sunlike α Cen A has been observed by successive generations of X-ray observatories for nearly four decades, albeit sporadically, there are key gaps in the coverage that affect the determination of the cycle period. In fact, the most recent pointings suggest a downturn in A's count rate that might be signaling a shorter, more solar-like cycle following a delayed minimum in the 2005--2010 time frame (perhaps an exaggerated version of the extended solar minimum between recent Cycles 23 and 24). Beyond the coronal cycles of the three stars, the sequence of periodic X-ray images represents a unique time-domain history concerning steady as well as variable sources in the two 30'x30' fields. The most conspicuous of the variable objects -- in the α Cen field -- will be described here.

New Worlds Airship

NASA Astrophysics Data System (ADS)

Harness, Anthony; Cash, Webster; Shipley, Ann; Glassman, Tiffany; Warwick, Steve

2013-09-01

We review the progress on the New Worlds Airship project, which has the eventual goal of suborbitally mapping the Alpha Centauri planetary system into the Habitable Zone. This project consists of a telescope viewing a star that is occulted by a starshade suspended from an airship. The starshade suppresses the starlight such that fainter planetary objects near the star are revealed. A visual sensor is used to determine the position of the starshade and keep the telescope within the starshade's shadow. In the first attempt to demonstrate starshades through astronomical observations, we have built a precision line of sight position indicator and flew it on a Zeppelin in October (2012). Since the airship provider went out of business we have been redesigning the project to use Vertical Takeoff Vertical Landing rockets instead. These Suborbital Reusable Launch Vehicles will serve as a starshade platform and test bed for further development of the visual sensor. We have completed ground tests of starshades on dry lakebeds and have shown excellent contrast. We are now attempting to use starshades on hilltops to occult stars and perform high contrast imaging of outer planetary systems such as the debris disk around Fomalhaut.

Techniques for High Contrast Imaging in Multi-Star Systems II: Multi-Star Wavefront Control

NASA Technical Reports Server (NTRS)

Sirbu, D.; Thomas, S.; Belikov, R.

2017-01-01

Direct imaging of exoplanets represents a challenge for astronomical instrumentation due to the high-contrast ratio and small angular separation between the host star and the faint planet. Multi-star systems pose additional challenges for coronagraphic instruments because of the diffraction and aberration leakage introduced by the additional stars, and as a result are not planned to be on direct imaging target lists. Multi-star wavefront control (MSWC) is a technique that uses a coronagraphic instrument's deformable mirror (DM) to create high-contrast regions in the focal plane in the presence of multiple stars. Our previous paper introduced the Super-Nyquist Wavefront Control (SNWC) technique that uses a diffraction grating to enable the DM to generate high-contrast regions beyond the nominal controllable region. These two techniques can be combined to generate high-contrast regions for multi-star systems at any angular separations. As a case study, a high-contrast wavefront control (WC) simulation that applies these techniques shows that the habitable region of the Alpha Centauri system can be imaged reaching 8 times 10(exp -9) mean contrast in 10 percent broadband light in one-sided dark holes from 1.6-5.5 lambda (wavelength) divided by D (distance).

Quantum vacuum polarization, nanotechnology and a robotic mission to Proxima Centauri

NASA Astrophysics Data System (ADS)

de Morais Mendonca Teles, Antonio

In order to achieve an interstellar flight mission it is necessary powerful propulsion technologies. The space between stars and the time for a flight are highly vast. As an example, the closest star to the Sun is α Cen C (known as Proxima Centauri) distant 4.2 light-years. It is a star with spectral type dM5e (a "reddish dwarf"), which makes part of a quasi-triple gravitational star system -together with α Cen A and α Cen B. Based on theoretical models and observa-tional data on stellar and planetary systems evolution, Proxima Centauri has the possibility of having a non-stellar companion (perhaps a Mars or Moon-sized object) orbiting close to it. So, here in this paper, I propose as a first interstellar flight reconnaissance mission, for testing new technologies and gathering of scientific data, it would be interesting a flyby-and-rendezvous mission to Proxima Centauri. . . Such mission, using nanotechnology and solar energy, could be achieved by one mini-spacecraft (the carrier with the propulsion mini-motors) and three smaller mini-spacecrafts inside -one for a flyby inside the star system, other (lighter) for orbital in-sertion around Proxima Centauri, and the other (attached to the lighter one) for landing on a possible Proxima Centauri's companion, based on observational data from the one in orbit. The reason for the use of nanotechnology is that it provides a large number of equipment inside a spacecraft, uses few energy for the internal processes of the mini-spacecrafts, can repair them-selves (nanotechnology-built materials are also shown as "intelligent" materials), and makes them with small inertial mass -important for relativistic matters. Solar energy is a powerful energy source -there are 3 stars making the α Cen system. Such technologies can obviously be also used to explore the Solar System. A mission to Proxima Centauri with a speed of 0.1 c takes 42 Earth years to arrive there. Knowing that the mini-spacecraft has to decelerate and the inertial mass of the mini-spacecraft has a relativistic increase factor of 0.005, fifty years of mission is a feasible one. A way of achieving this is by using altogether the possible available spacecraft acceleration: gravity assistance, ionic propulsion, and using characteristics of the medium through which any spacecrafts travel by -vacuum. Vacuum has intrinsic quantum properties such as quantum tunneling, latent quantum residual energy, and the quantum vac-uum polarization phenomenon. I also propose the use of such quantum vacuum polarization (QVP) for the propulsion assistance for possible future Solar System and interstellar missions. QVP is a natural phenomenon arisen as a second-order correction for perturbation of quantum vacuum fluctuations, within the quantum field physics arena. It is related experimentally to the Casimir effect (the appearance of a negative potential barrier between very close and par-allel metallic plates in vacuum). Using a laser beam with a minimum of 1.22 MeV energy it is possible to create inside those plates in vacuum 1 real pair of electron-positron (anti-electron), and associated with this there is the creation of 1 virtual pair of electron-positron, through the geometrodynamical arrangement of the quantum vacuum fluctuations states, with a very small interval of time (δt). With much greater energies (GeV, TeV) it is possible to create virtual pairs with much longer δt, with the appearance of a repulsive force between the real and asso-ciated virtual pairs, caused by forced alignment of the spins of the real and virtual pairs. This could be attained by the use of a magnetic field. A powerful laser put in the extremity of the mini-spacecraft (together with the ionic mini-motor) in the middle of Casimir plates, could use that repulsive force to get much more momentum to the mini-spacecraft, for a possible speed in the order of 0.1 c. Telecommunication aspect can be arranged through the use of a tracking and data relay mini-satellites system orbiting the Sun.

The Kapteyn Moving Group Is Not Tidal Debris From ω Centauri

NASA Astrophysics Data System (ADS)

Navarrete, Camila; Chanamé, Julio; Ramírez, Iván; Meza, Andrés; Anglada-Escudé, Guillem; Shkolnik, Evgenya

2015-07-01

The Kapteyn moving group has been postulated as tidal debris from ω Centauri. If true, members of the group should show some of the chemical abundance patterns known for stars in the cluster. We present an optical and near-infrared high-resolution, high-signal-to-noise ratio spectroscopic study of 14 stars of the Kapteyn group, plus 10 additional stars (the ω Cen group) that, while not listed as members of the Kapteyn group as originally defined, have nevertheless been associated dynamically with ω Centauri. Abundances for Na, O, Mg, Al, Ca, and Ba were derived from the optical spectra, while the strength of the chromospheric He i 10830 Å line is studied as a possible helium abundance indicator. The resulting Na-O and Mg-Al patterns for stars of the combined Kapteyn and ω Cen group samples do not resemble those of ω Centauri, and are not different from those of field stars of the Galactic halo. The distribution of equivalent widths of the He i 10830 Å line is consistent with that found among non-active field stars. Therefore, no evidence is found for second-generation stars within our samples, which most likely rules out a globular-cluster origin. Moreover, no hint of the unique barium overabundance at the metal-rich end, well established for ω Centauri stars, is seen among stars of the combined samples. Because this specific Ba pattern is present in ω Centauri irrespective of stellar generation, this would rule out the possibility that our entire sample might be composed of only first-generation stars from the cluster. Finally, for the stars of the Kapteyn group, the possibility of an origin in the hypothetical parent galaxy of ω Centauri is disfavored by the different run of α-elements with metallicity between our targets and stars from present-day dwarf galaxies. Based on observations collected at the European Southern Observatory, Chile (ESO Program 090.B-0605) and observations gathered with the 6.5 m Magellan Telescopes at Las Campanas Observatory, Chile.

Discovery of New Retrograde Substructures: The Shards of ω Centauri?

NASA Astrophysics Data System (ADS)

Myeong, G. C.; Evans, N. W.; Belokurov, V.; Sanders, J. L.; Koposov, S. E.

2018-06-01

We use the SDSS-Gaia catalogue to search for substructure in the stellar halo. The sample comprises 62 133 halo stars with full phase space coordinates and extends out to heliocentric distances of ˜10 kpc. As actions are conserved under slow changes of the potential, they permit identification of groups of stars with a common accretion history. We devise a method to identify halo substructures based on their clustering in action space, using metallicity as a secondary check. This is validated against smooth models and numerical constructed stellar halos from the Aquarius simulations. We identify 21 substructures in the SDSS-Gaia catalogue, including 7 high significance, high energy and retrograde ones. We investigate whether the retrograde substructures may be material stripped off the atypical globular cluster ω Centauri. Using a simple model of the accretion of the progenitor of the ω Centauri, we tentatively argue for the possible association of up to 5 of our new substructures (labelled Rg1, Rg3, Rg4, Rg6 and Rg7) with this event. This sets a minimum mass of 5× 108M⊙ for the progenitor, so as to bring ω Centauri to its current location in action - energy space. Our proposal can be tested by high resolution spectroscopy of the candidates to look for the unusual abundance patterns possessed by ω Centauri stars.

Photogravimagnetic assists of light sails: a mixed blessing for Breakthrough Starshot?

NASA Astrophysics Data System (ADS)

Forgan, Duncan H.; Heller, René; Hippke, Michael

2018-03-01

Upon entering a star system, light sails are subject to both gravitational forces and radiation pressure, and can use both in concert to modify their trajectory. Moreover, stars possess significant magnetic fields, and if the sail is in any way charged, it will feel the Lorentz force also. We investigate the dynamics of so-called `photogravimagnetic assists' of sailcraft around α Centauri A, a potential first destination en route to Proxima Centauri (the goal of the Breakthrough Starshot programme). We find that a 10-m2 sail with a charge-to-mass ratio of around 10 μC g-1 or higher will need to take account of magnetic field effects during orbital manoeuvres. The magnetic field can provide an extra source of deceleration and deflection, and allow capture on to closer orbits around a target star. However, flipping the sign of the sailcraft's charge can radically change resulting trajectories, resulting in complex loop-de-loops around magnetic field lines and essentially random ejection from the star system. Even on well-behaved trajectories, the field can generate off-axis deflections at α Centauri that, while minor, can result in very poor targeting of the final destination (Proxima) post-assist. Fortunately for Breakthrough Starshot, nanosails are less prone to charging en route than their heavier counterparts, but can still accrue relatively high charge at both the origin and destination, when travelling at low speeds. Photogravimagnetic assists are highly non-trivial, and require careful course correction to mitigate against unwanted changes in trajectory.

Historical Reveiw of Interstellar Probe Concepts and Examination of Payload Mass Considerations for Different System Architectures

NASA Astrophysics Data System (ADS)

Long, K.

2017-12-01

The ability to send a space probe beyond the Voyager probes, through the interstellar medium and towardsthe distant stars, has long been the ambition of both the science ction literature but also a small community ofadvocates that have argued for a broader and deeper vision of space exploration that goes outside of our SolarSystem. In this paper we discuss some of the historical interstellar probe concepts which are propelled usingdierent types of propulsion technology, from energetic reaction engines to directed energy beaming, and considerthe payload mass associated with such concepts. We compare and contrast the dierent design concepts, payloadmass fractions, powers and energies and discuss the implications for robotic space exploration within the stellarneighbourhood. Finally, we consider the Breakthrough Starshot initiative, which proposes to send a Gram-scalelaser driven spacecraft to the Alpha Centauri system in a 20 year mission travelling at v 0.2c. We show howthis is a good start in pushing our robotic probes towards interstellar destinations, but also discuss the potentialfor scaling up this systems architecture to missions closer at home, or higher mass missions wider aeld. This is apresentation for the American Geophysical Union at the AGU Fall meeting, New Orleans, 11-15 December 2017,Special Session on the Interstellar Probe Missions.Keywords: Interstellar Probe, Breakthrough Starshot

Open Air Rock Art Between Alva and Ceira Rivers: a Voyage Through Mining, Trading, Transhumance Routes and the Orientation in the Landscape

NASA Astrophysics Data System (ADS)

Pimenta, F.; Ribeiro, N.; Smith, A.; Joaquinito, A.; Pereira, S.; Tirapicos, L.

2015-05-01

Open air rock carvings, the object of the present study, were artistic expressions made by the itinerant shepherds, miners, traders and the resident populations along trading and transhumance routes and nearby areas. These rock art sites were used as markers to denote routes or territorial claims and in some cases may have been associated with ritual celebrations. In this paper we will present the results of the analysis of the orientations in the landscape of 688 engraved outcrops, distributed in 11 mountainous areas between the Alva and Ceira rivers. We will discuss possible solsticial markers and will address a possible relationship between the orientation of the podomorph carvings with the Summer Full Moon or Alpha Centauri.

The Alpha Centauri binary system. Atmospheric parameters and element abundances

NASA Astrophysics Data System (ADS)

Porto de Mello, G. F.; Lyra, W.; Keller, G. R.

2008-09-01

Context: The α Centauri binary system, owing to its duplicity, proximity and brightness, and its components' likeness to the Sun, is a fundamental calibrating object for the theory of stellar structure and evolution and the determination of stellar atmospheric parameters. This role, however, is hindered by a considerable disagreement in the published analyses of its atmospheric parameters and abundances. Aims: We report a new spectroscopic analysis of both components of the α Centauri system, compare published analyses of the system, and attempt to quantify the discrepancies still extant in the determinations of the atmospheric parameters and abundances of these stars. Methods: The analysis is differential with respect to the Sun, based on spectra with R = 35 000 and signal-to-noise ratio ≥1000, and employed spectroscopic and photometric methods to obtain as many independent T_eff determinations as possible. We also check the atmospheric parameters for consistency against the results of the dynamical analysis and the positions of the components in a theoretical HR diagram. Results: The spectroscopic atmospheric parameters of the system are found to be T_eff = (5847 ± 27) K, [Fe/H] = +0.24 ± 0.03, log g = 4.34 ± 0.12, and ξt = 1.46 ± 0.03 km s-1, for α Cen A, and T_eff = (5316 ± 28) K, [Fe/H] = +0.25 ± 0.04, log g = 4.44 ± 0.15, and ξt = 1.28 ± 0.15 km s^-1 for α Cen B. The parameters were derived from the simultaneous excitation & ionization equilibria of Fe I and Fe II lines. T_effs were also obtained by fitting theoretical profiles to the Hα line and from photometric calibrations. Conclusions: We reached good agreement between the three criteria for α Cen A. For α Cen B the spectroscopic T_eff is ~140 K higher than the other two determinations. We discuss possible origins of this inconsistency, concluding that the presence of non-local thermodynamic equilibrium effects is a probable candidate, but we note that there is as yet no consensus on the existence and cause of an offset between the spectroscopic and photometric T_eff scales of cool dwarfs. The spectroscopic surface gravities also agree with those derived from directly measured masses and radii. An average of three independent T_eff criteria leads to T_eff (A) = (5824 ± 26) K and T_eff (B) = (5223 ± 62) K. The abundances of Na, Mg, Si, Mn, Co, and Ni and, possibly, Cu are significantly enriched in the system, which also seems to be deficient in Y and Ba. This abundance pattern can be deemed normal in the context of recent data on metal-rich stars. The position of α Cen A in an up-to-date theoretical evolutionary diagram yields a good match of the evolutionary mass and age (in the 4.5 to 5.3 Gyr range) with those from the dynamical solution and seismology, but only marginal agreement for α Cen B, taking into account its more uncertain T_eff. Based on observations collected at Observatório do Pico dos Dias (OPD), operated by the Laboratório Nacional de Astrofísica, CNPq, Brazil. Table 2 is only available in electronic form at http://www.aanda.org

MAGNETIC CYCLES IN A DYNAMO SIMULATION OF FULLY CONVECTIVE M-STAR PROXIMA CENTAURI

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yadav, Rakesh K.; Wolk, Scott J.; Christensen, Ulrich R.

2016-12-20

The recent discovery of an Earth-like exoplanet around Proxima Centauri has shined a spot light on slowly rotating fully convective M-stars. When such stars rotate rapidly (period ≲20 days), they are known to generate very high levels of activity that is powered by a magnetic field much stronger than the solar magnetic field. Recent theoretical efforts are beginning to understand the dynamo process that generates such strong magnetic fields. However, the observational and theoretical landscape remains relatively uncharted for fully convective M-stars that rotate slowly. Here, we present an anelastic dynamo simulation designed to mimic some of the physical characteristicsmore » of Proxima Centauri, a representative case for slowly rotating fully convective M-stars. The rotating convection spontaneously generates differential rotation in the convection zone that drives coherent magnetic cycles where the axisymmetric magnetic field repeatedly changes polarity at all latitudes as time progress. The typical length of the “activity” cycle in the simulation is about nine years, in good agreement with the recently proposed activity cycle length of about seven years for Proxima Centauri. Comparing our results with earlier work, we hypothesis that the dynamo mechanism undergoes a fundamental change in nature as fully convective stars spin down with age.« less

An Earth-mass planet orbiting α Centauri B.

PubMed

Dumusque, Xavier; Pepe, Francesco; Lovis, Christophe; Ségransan, Damien; Sahlmann, Johannes; Benz, Willy; Bouchy, François; Mayor, Michel; Queloz, Didier; Santos, Nuno; Udry, Stéphane

2012-11-08

Exoplanets down to the size of Earth have been found, but not in the habitable zone--that is, at a distance from the parent star at which water, if present, would be liquid. There are planets in the habitable zone of stars cooler than our Sun, but for reasons such as tidal locking and strong stellar activity, they are unlikely to harbour water-carbon life as we know it. The detection of a habitable Earth-mass planet orbiting a star similar to our Sun is extremely difficult, because such a signal is overwhelmed by stellar perturbations. Here we report the detection of an Earth-mass planet orbiting our neighbour star α Centauri B, a member of the closest stellar system to the Sun. The planet has an orbital period of 3.236 days and is about 0.04 astronomical units from the star (one astronomical unit is the Earth-Sun distance).

Terrestrial Planet Formation Around Close Binary Stars

NASA Technical Reports Server (NTRS)

Lissauer, Jack J.; Quintana, Elisa V.

2003-01-01

Most stars reside in multiple star systems; however, virtually all models of planetary growth have assumed an isolated single star. Numerical simulations of the collapse of molecular cloud cores to form binary stars suggest that disks will form within such systems. Observations indirectly suggest disk material around one or both components within young binary star systems. If planets form at the right places within such circumstellar disks, they can remain in stable orbits within the binary star systems for eons. We are simulating the late stages of growth of terrestrial planets around close binary stars, using a new, ultrafast, symplectic integrator that we have developed for this purpose. The sum of the masses of the two stars is one solar mass, and the initial disk of planetary embryos is the same as that used for simulating the late stages of terrestrial planet growth within our Solar System and in the Alpha Centauri wide binary star system. Giant planets &are included in the simulations, as they are in most simulations of the late stages of terrestrial planet accumulation in our Solar System. When the stars travel on a circular orbit with semimajor axis of up to 0.1 AU about their mutual center of mass, the planetary embryos grow into a system of terrestrial planets that is statistically identical to those formed about single stars, but a larger semimajor axis and/or a significantly eccentric binary orbit can lead to significantly more dynamically hot terrestrial planet systems.

Omega Centauri Looks Radiant in Infrared

NASA Technical Reports Server (NTRS)

2008-01-01

[figure removed for brevity, see original site] Poster Version

A cluster brimming with millions of stars glistens like an iridescent opal in this image from NASA's Spitzer Space Telescope. Called Omega Centauri, the sparkling orb of stars is like a miniature galaxy. It is the biggest and brightest of the 150 or so similar objects, called globular clusters, that orbit around the outside of our Milky Way galaxy. Stargazers at southern latitudes can spot the stellar gem with the naked eye in the constellation Centaurus.

Globular clusters are some of the oldest objects in our universe. Their stars are over 12 billion years old, and, in most cases, formed all at once when the universe was just a toddler. Omega Centauri is unusual in that its stars are of different ages and possess varying levels of metals, or elements heavier than boron. Astronomers say this points to a different origin for Omega Centauri than other globular clusters: they think it might be the core of a dwarf galaxy that was ripped apart and absorbed by our Milky Way long ago.

In this new view of Omega Centauri, Spitzer's infrared observations have been combined with visible-light data from the National Science Foundation's Blanco 4-meter telescope at Cerro Tololo Inter-American Observatory in Chile. Visible-light data with a wavelength of .55 microns is colored blue, 3.6-micron infrared light captured by Spitzer's infrared array camera is colored green and 24-micron infrared light taken by Spitzer's multiband imaging photometer is colored red.

Where green and red overlap, the color yellow appears. Thus, the yellow and red dots are stars revealed by Spitzer. These stars, called red giants, are more evolved, larger and dustier. The stars that appear blue were spotted in both visible and 3.6-micron-, or near-, infrared light. They are less evolved, like our own sun. Some of the red spots in the picture are distant galaxies beyond our own.

Spitzer found very little dust around any but the most luminous, coolest red giants, implying that the dimmer red giants do not form significant amounts of dust. The space between the stars in Omega Centauri was also found to lack dust, which means the dust is rapidly destroyed or leaves the cluster.

Space Telescope Design to Directly Image the Habitable Zone of Alpha Centauri

NASA Technical Reports Server (NTRS)

Bendek, Eduardo A.; Belikov, Ruslan; Lozi, Julien; Thomas, Sandrine; Males, Jared; Weston, Sasha; McElwain, Michael

2015-01-01

The scientific interest in directly imaging and identifying Earth-like planets within the Habitable Zone (HZ) around nearby stars is driving the design of specialized direct imaging missions such as ACESAT, EXO-C, EXO-S and AFTA-C. The inner edge of Alpha Cen A&B Habitable Zone is found at exceptionally large angular separations of 0.7" and 0.4" respectively. This enables direct imaging of the system with a 0.3m class telescope. Contrast ratios on the order of 10(exp 10) are needed to image Earth-brightness planets. Low-resolution (5-band) spectra of all planets may allow establishing the presence and amount of an atmosphere. This star system configuration is optimal for a specialized small, and stable space telescope that can achieve high-contrast but has limited resolution. This paper describes an innovative instrument design and a mission concept based on a full Silicon Carbide off-axis telescope, which has a Phase Induced Amplitude Apodization coronagraph embedded in the telescope. This architecture maximizes stability and throughput. A Multi-Star Wave Front algorithm is implemented to drive a deformable mirror controlling simultaneously diffracted light from the on-axis and binary companion star. The instrument has a Focal Plane Occulter to reject starlight into a high precision pointing control camera. Finally we utilize a Orbital Differential Imaging (ODI) post-processing method that takes advantage of a highly stable environment (Earth-trailing orbit) and a continuous sequence of images spanning 2 years, to reduce the final noise floor in post processing to approximately 2e-11 levels, enabling high confidence and at least 90% completeness detections of Earth-like planets.

Superhabitable worlds.

PubMed

Heller, René; Armstrong, John

2014-01-01

To be habitable, a world (planet or moon) does not need to be located in the stellar habitable zone (HZ), and worlds in the HZ are not necessarily habitable. Here, we illustrate how tidal heating can render terrestrial or icy worlds habitable beyond the stellar HZ. Scientists have developed a language that neglects the possible existence of worlds that offer more benign environments to life than Earth does. We call these objects "superhabitable" and discuss in which contexts this term could be used, that is to say, which worlds tend to be more habitable than Earth. In an appendix, we show why the principle of mediocracy cannot be used to logically explain why Earth should be a particularly habitable planet or why other inhabited worlds should be Earth-like. Superhabitable worlds must be considered for future follow-up observations of signs of extraterrestrial life. Considering a range of physical effects, we conclude that they will tend to be slightly older and more massive than Earth and that their host stars will likely be K dwarfs. This makes Alpha Centauri B, which is a member of the closest stellar system to the Sun and is supposed to host an Earth-mass planet, an ideal target for searches for a superhabitable world.

A BIRD'S EYE VIEW OF A GALAXY COLLISION

NASA Technical Reports Server (NTRS)

2002-01-01

What appears as a bird's head, leaning over to snatch up a tasty meal, is a striking example of a galaxy collision in NGC 6745. A large spiral galaxy, with its nucleus still intact, peers at the smaller passing galaxy (nearly out of the field of view at lower right), while a bright blue beak and bright whitish-blue top feathers show the distinct path taken during the smaller galaxy's journey. These galaxies did not merely interact gravitationally as they passed one another, they actually collided. When galaxies collide, the stars that normally comprise the major portion of the luminous mass of each of the two galaxies will almost never collide with each other, but will pass rather freely between each other with little damage. This occurs because the physical size of individual stars is tiny compared to their typical separations, making the chance of physical encounter relatively small. In our own Milky Way galaxy, the space between our Sun and our nearest stellar neighbor, Proxima Centauri (part of the Alpha Centauri triple system), is a vast 4.3 light-years. However, the situation is quite different for the interstellar media in the above two galaxies - material consisting largely of clouds of atomic and molecular gases and of tiny particles of matter and dust, strongly coupled to the gas. Wherever the interstellar clouds of the two galaxies collide, they do not freely move past each other without interruption but, rather, suffer a damaging collision. High relative velocities cause ram pressures at the surface of contact between the interacting interstellar clouds. This pressure, in turn, produces material densities sufficiently extreme as to trigger star formation through gravitational collapse. The hot blue stars in this image are evidence of this star formation. This image was created by the Hubble Heritage Team using NASA Hubble Space Telescope archive data taken with the Wide Field Planetary Camera 2 in March 1996. Members of the science team, which include Roger Lynds (KPNO/NOAO) and Earl J. O'Neil, Jr. (Steward Obs.), used infrared, red, visual and ultravoilet filters to image this galaxy system. Lynds and O'Neil are currently using the Hubble data along with ground-based radio observations to further study the interactions within NGC 6745. Image Credit: NASA and The Hubble Heritage Team (STScI/AURA) Acknowledgment: Roger Lynds (KPNO/NOAO)

Examining the possibility of magnetic protection of Proxima b's atmosphere

NASA Astrophysics Data System (ADS)

Garcia-Sage, K.; Glocer, A.; Drake, J. J.; Gronoff, G.; Cohen, O.

2017-12-01

It is commonly believed that magnetic field provides protection of the planet's atmosphere from space weather effects. However, escape of the ionosphere along open magnetic field lines at the poles may under certain conditions be quite large and involve the escape of heavy ions like O+. The EUV spectrum of the star, in particular, produces ionization and heating that enhances escape. We calculate the field-aligned ionospheric escape for a reconstructed spectrum from Proxima Centauri. The EUV flux at the orbit of Proxima b is two orders of magnitude higher than at Earth. We model the resulting mass loss rates, assuming an Earth-like atmosphere and magnetic field. we also show uncertainties due to neutral atmospheric temperatures and polar cap size. We show that for high levels of stellar activity, the mass loss timescales for an Earth-like atmosphere are less than the age of the Proxima Centauri system, casting doubt on the idea that a magnetic field can protect a planet from space weather-driven atmospheric loss.

A Dynamical N-body model for the central region of ω Centauri

NASA Astrophysics Data System (ADS)

Jalali, B.; Baumgardt, H.; Kissler-Patig, M.; Gebhardt, K.; Noyola, E.; Lützgendorf, N.; de Zeeuw, P. T.

2012-02-01

Context. Supermassive black holes (SMBHs) are fundamental keys to understand the formation and evolution of their host galaxies. However, the formation and growth of SMBHs are not yet well understood. One of the proposed formation scenarios is the growth of SMBHs from seed intermediate-mass black holes (IMBHs, 102 to 105 M⊙) formed in star clusters. In this context, and also with respect to the low mass end of the M• - σ relation for galaxies, globular clusters are in a mass range that make them ideal systems to look for IMBHs. Among Galactic star clusters, the massive cluster ω Centauri is a special target due to its central high velocity dispersion and also its multiple stellar populations. Aims: We study the central structure and dynamics of the star cluster ω Centauri to examine whether an IMBH is necessary to explain the observed velocity dispersion and surface brightness profiles. Methods: We perform direct N-body simulations on GPU and GRAPE special purpose computers to follow the dynamical evolution of ω Centauri. The simulations are compared to the most recent data-sets in order to explain the present-day conditions of the cluster and to constrain the initial conditions leading to the observed profiles. Results: We find that starting from isotropic spherical multi-mass King models and within our canonical assumptions, a model with a central IMBH mass of 2% of the cluster stellar mass, i.e. a 5. × 104 M⊙ IMBH, provides a satisfactory fit to both the observed shallow cusp in surface brightness and the continuous rise towards the center of the radial velocity dispersion profile. In our isotropic spherical models, the predicted proper motion dispersion for the best-fit model is the same as the radial velocity dispersion one. Conclusions: We conclude that with the presence of a central IMBH in our models, we reproduce consistently the rise in the radial velocity dispersion. Furthermore, we always end up with a shallow cusp in the projected surface brightness of our model clusters containing an IMBH. In addition, we find that the M/L ratio seems to be constant in the central region, and starts to rise slightly from the core radius outwards for all models independent of the presence of a black hole. Considering our initial parameter space, it is not possible to explain the observations without a central IMBH for ω Centauri. To further strengthen the presence of an IMBH as a unique explanation of the observed light and kinematics more detailed analysis such as investigating the contribution of primordial binaries and different anisotropy profiles should be studied.

A Multi-year Search for Transits of Proxima Centauri. I. Light Curves Corresponding to Published Ephemerides

NASA Astrophysics Data System (ADS)

Blank, David L.; Feliz, Dax; Collins, Karen A.; White, Graeme L.; Stassun, Keivan G.; Curtis, Ivan A.; Hart, Rhodes; Kielkopf, John F.; Nelson, Peter; Relles, Howard; Stockdale, Christopher; Jayawardene, Bandupriya; Pennypacker, Carlton R.; Shankland, Paul; Reichart, Daniel E.; Haislip, Joshua B.; Kouprianov, Vladimir V.

2018-06-01

Proxima Centauri has become the subject of intense study since the radial-velocity (RV) discovery by Anglada-Escudé et al. of a planet orbiting this nearby M dwarf every ∼11.2 days. If Proxima Centauri b transits its host star, independent confirmation of its existence is possible, and its mass and radius can be measured in units of the stellar host mass and radius. To date, there have been three independent claims of possible transit-like event detections in light curve observations obtained by the MOST satellite (in 2014–15), the Bright Star Survey Telescope telescope in Antarctica (in 2016), and the Las Campanas Observatory (in 2016). The claimed possible detections are tentative, due in part to the variability intrinsic to the host star, and in the case of the ground-based observations, also due to the limited duration of the light curve observations. Here, we present preliminary results from an extensive photometric monitoring campaign of Proxima Centauri, using telescopes around the globe and spanning from 2006 to 2017, comprising a total of 329 observations. Considering our data that coincide directly and/or phased with the previously published tentative transit detections, we are unable to independently verify those claims. We do, however, verify the previously reported ubiquitous and complex variability of the host star. We discuss possible interpretations of the data in light of the previous claims, and we discuss future analyses of these data that could more definitively verify or refute the presence of transits associated with the RV-discovered planet.

α Centauri A as a potential stellar model calibrator: establishing the nature of its core

NASA Astrophysics Data System (ADS)

Nsamba, B.; Monteiro, M. J. P. F. G.; Campante, T. L.; Cunha, M. S.; Sousa, S. G.

2018-05-01

Understanding the physical process responsible for the transport of energy in the core of α Centauri A is of the utmost importance if this star is to be used in the calibration of stellar model physics. Adoption of different parallax measurements available in the literature results in differences in the interferometric radius constraints used in stellar modelling. Further, this is at the origin of the different dynamical mass measurements reported for this star. With the goal of reproducing the revised dynamical mass derived by Pourbaix & Boffin, we modelled the star using two stellar grids varying in the adopted nuclear reaction rates. Asteroseismic and spectroscopic observables were complemented with different interferometric radius constraints during the optimisation procedure. Our findings show that best-fit models reproducing the revised dynamical mass favour the existence of a convective core (≳ 70% of best-fit models), a result that is robust against changes to the model physics. If this mass is accurate, then α Centauri A may be used to calibrate stellar model parameters in the presence of a convective core.

A terrestrial planet candidate in a temperate orbit around Proxima Centauri

NASA Astrophysics Data System (ADS)

Anglada-Escudé, Guillem; Amado, Pedro J.; Barnes, John; Berdiñas, Zaira M.; Butler, R. Paul; Coleman, Gavin A. L.; de La Cueva, Ignacio; Dreizler, Stefan; Endl, Michael; Giesers, Benjamin; Jeffers, Sandra V.; Jenkins, James S.; Jones, Hugh R. A.; Kiraga, Marcin; Kürster, Martin; López-González, María J.; Marvin, Christopher J.; Morales, Nicolás; Morin, Julien; Nelson, Richard P.; Ortiz, José L.; Ofir, Aviv; Paardekooper, Sijme-Jan; Reiners, Ansgar; Rodríguez, Eloy; Rodríguez-López, Cristina; Sarmiento, Luis F.; Strachan, John P.; Tsapras, Yiannis; Tuomi, Mikko; Zechmeister, Mathias

2016-08-01

At a distance of 1.295 parsecs, the red dwarf Proxima Centauri (α Centauri C, GL 551, HIP 70890 or simply Proxima) is the Sun’s closest stellar neighbour and one of the best-studied low-mass stars. It has an effective temperature of only around 3,050 kelvin, a luminosity of 0.15 per cent of that of the Sun, a measured radius of 14 per cent of the radius of the Sun and a mass of about 12 per cent of the mass of the Sun. Although Proxima is considered a moderately active star, its rotation period is about 83 days (ref. 3) and its quiescent activity levels and X-ray luminosity are comparable to those of the Sun. Here we report observations that reveal the presence of a small planet with a minimum mass of about 1.3 Earth masses orbiting Proxima with a period of approximately 11.2 days at a semi-major-axis distance of around 0.05 astronomical units. Its equilibrium temperature is within the range where water could be liquid on its surface.

A terrestrial planet candidate in a temperate orbit around Proxima Centauri.

PubMed

Anglada-Escudé, Guillem; Amado, Pedro J; Barnes, John; Berdiñas, Zaira M; Butler, R Paul; Coleman, Gavin A L; de la Cueva, Ignacio; Dreizler, Stefan; Endl, Michael; Giesers, Benjamin; Jeffers, Sandra V; Jenkins, James S; Jones, Hugh R A; Kiraga, Marcin; Kürster, Martin; López-González, Marίa J; Marvin, Christopher J; Morales, Nicolás; Morin, Julien; Nelson, Richard P; Ortiz, José L; Ofir, Aviv; Paardekooper, Sijme-Jan; Reiners, Ansgar; Rodríguez, Eloy; Rodrίguez-López, Cristina; Sarmiento, Luis F; Strachan, John P; Tsapras, Yiannis; Tuomi, Mikko; Zechmeister, Mathias

2016-08-25

At a distance of 1.295 parsecs, the red dwarf Proxima Centauri (α Centauri C, GL 551, HIP 70890 or simply Proxima) is the Sun's closest stellar neighbour and one of the best-studied low-mass stars. It has an effective temperature of only around 3,050 kelvin, a luminosity of 0.15 per cent of that of the Sun, a measured radius of 14 per cent of the radius of the Sun and a mass of about 12 per cent of the mass of the Sun. Although Proxima is considered a moderately active star, its rotation period is about 83 days (ref. 3) and its quiescent activity levels and X-ray luminosity are comparable to those of the Sun. Here we report observations that reveal the presence of a small planet with a minimum mass of about 1.3 Earth masses orbiting Proxima with a period of approximately 11.2 days at a semi-major-axis distance of around 0.05 astronomical units. Its equilibrium temperature is within the range where water could be liquid on its surface.

The HST Large Programme on ω Centauri. II. Internal Kinematics

NASA Astrophysics Data System (ADS)

Bellini, Andrea; Libralato, Mattia; Bedin, Luigi R.; Milone, Antonino P.; van der Marel, Roeland P.; Anderson, Jay; Apai, Dániel; Burgasser, Adam J.; Marino, Anna F.; Rees, Jon M.

2018-01-01

In this second installment of the series, we look at the internal kinematics of the multiple stellar populations of the globular cluster ω Centauri in one of the parallel Hubble Space Telescope (HST) fields, located at about 3.5 half-light radii from the center of the cluster. Thanks to the over 15 yr long baseline and the exquisite astrometric precision of the HST cameras, well-measured stars in our proper-motion catalog have errors as low as ∼10 μas yr‑1, and the catalog itself extends to near the hydrogen-burning limit of the cluster. We show that second-generation (2G) stars are significantly more radially anisotropic than first-generation (1G) stars. The latter are instead consistent with an isotropic velocity distribution. In addition, 1G stars have excess systemic rotation in the plane of the sky with respect to 2G stars. We show that the six populations below the main-sequence (MS) knee identified in our first paper are associated with the five main population groups recently isolated on the upper MS in the core of cluster. Furthermore, we find both 1G and 2G stars in the field to be far from being in energy equipartition, with {η }1{{G}}=-0.007+/- 0.026 for the former and {η }2{{G}}=0.074+/- 0.029 for the latter, where η is defined so that the velocity dispersion {σ }μ scales with stellar mass as {σ }μ \\propto {m}-η . The kinematical differences reported here can help constrain the formation mechanisms for the multiple stellar populations in ω Centauri and other globular clusters. We make our astro-photometric catalog publicly available.

Direct Evidence for an Enhancement of Helium in Giant Stars in Omega Centauri

NASA Astrophysics Data System (ADS)

Dupree, A. K.; Strader, Jay; Smith, Graeme H.

2011-02-01

The double main sequence identified in the globular cluster Omega Centauri has been interpreted using isochrones to indicate a large variation in the abundance of helium. If true, a helium enhancement carries strong implications for the chemical and stellar evolutionary history of this cluster. However, only indirect measures currently support this conjecture. We report the discovery of a variation in the line strength of the near-infrared He I 10830 Å transition in 12 similar red giants in Omega Centauri observed with PHOENIX on Gemini-S. Abundances of these stars derived from Magellan/MIKE spectra taken at the Las Campanas Observatory show that the helium transition is not detected in the most metal-poor population ([Fe/H] < -1.8), yet is present in the majority of stars with [Fe/H] >= -1.8. These observations give the first direct evidence for an enhancement of helium in Omega Centauri. The appearance of helium appears better correlated with increased [Al/Fe] and [Na/Fe] abundances than as a function of [Fe/H], giving observational support to the presence of high-temperature H burning in a prior generation of stars. Data presented herein were obtained at the Gemini Observatory, which is operated by the Association of Universities for Research in Astronomy, Inc., under a cooperative agreement with the NSF on behalf of the Gemini partnership: the National Science Foundation (United States), the Science and Technology Facilities Council (United Kingdom), the National Research Council (Canada), CONICYT (Chile), the Australian Research Council (Australia), Ministério da Ciência e Tecnologia (Brazil), and Ministerio de Ciencia, Tecnología e Innovación Productiva (Argentina). This paper also includes spectra gathered with the 6.5 m Magellan Telescope/CLAY located at Las Campanas Observatory, Chile.

Stability of triangular points in the elliptic restricted three-body problem with oblateness up to zonal harmonic J4 of both primaries

NASA Astrophysics Data System (ADS)

Singh, Jagadish; Tyokyaa, Richard K.

2016-10-01

In this paper, we study the locations and stability of triangular points in the elliptic restricted three-body problem when both primaries are taken as oblate spheroids with oblateness up to J4. The positions of the triangular points are seen to be affected by oblateness of the primaries and the eccentricity of their orbits. The triangular points are conditionally stable for 0<μ<μc0<μ<μc and unstable for μc≤μ≤12μc≤μ≤12, where μcμc is the critical mass parameter depending on the oblateness coefficients J2iJ2i (i =1,2) and the eccentricity of the orbits. We further observe that both coefficients J2 and J4, semi-major axis and the eccentricity have destabilizing tendencies resulting in a decrease in the size of the region of stability with an increase in the parameters involved. Knowing that, in general, the triangular equilibrium points are stable for 0<μ<μc0<μ<μc, in particular systems (Alpha Centauri, X1X1 Bootis, Sirius and Kruger 60) this does not hold and such points are unstable.

Stars

NASA Astrophysics Data System (ADS)

Capelato, Hugo Vicente

1999-01-01

We will begin our study with a more or less superficial inspection of the "forest" of stars that we see in the skies. The first thing we notice is that, as sources of light, they are much weaker than the Sun. Second, their apparent colors vary; from a bluish-white in most of them to a reddish-yellow, which is rarer. There is also a third aspect, though it is not very obvious to the naked eye: most of the stars group themselves in small families of two, three or more members. A good example is the Alpha Centauri, the closest star to us, which, in fact, is a triple system of stars. Another is the group of 7 stars that make up the Pleiades, which will be discussed later on. In fact, almost half of the stars are double systems with only two members, called binary stars. Most of these double stars, though together, are separated by several astronomical units (one astronomical unit, AU, is the distance from Earth to the sun: see Chapter 1), and revolve around each other over periods of several years. And yet the revolutions of some binary stars, separated by much smaller distances, occur in only a few hours! These stars are so close to each other that they can share enveloping material. Often this exchange occurs in a somewhat violent manner. Local explosions may occur, expelling matter away from the system. In other binary systems, where one of the components is a very compact, dense star, companion material flows more calmly, making up a light disk around the compact star.

TECHNIQUES FOR HIGH-CONTRAST IMAGING IN MULTI-STAR SYSTEMS. I. SUPER-NYQUIST WAVEFRONT CONTROL

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thomas, S.; Belikov, R.; Bendek, E.

2015-09-01

Direct imaging of extra-solar planets is now a reality with the deployment and commissioning of the first generation of specialized ground-based instruments (GPI, SPHERE, P1640, and SCExAO). These systems allow of planets 10{sup 7} times fainter than their host star. For space-based missions (EXCEDE, EXO-C, EXO-S, WFIRST), various teams have demonstrated laboratory contrasts reaching 10{sup −10} within a few diffraction limits from the star. However, all of these current and future systems are designed to detect faint planets around a single host star, while most non-M-dwarf stars such as Alpha Centauri belong to multi-star systems. Direct imaging around binaries/multiple systemsmore » at a level of contrast allowing detection of Earth-like planets is challenging because the region of interest is contaminated by the host star's companion in addition to the host itself. Generally, the light leakage is caused by both diffraction and aberrations in the system. Moreover, the region of interest usually falls outside the correcting zone of the deformable mirror (DM) with respect to the companion. Until now, it has been thought that removing the light of a companion star is too challenging, leading to the exclusion of many binary systems from target lists of direct imaging coronographic missions. In this paper, we will show new techniques for high-contrast imaging of planets around multi-star systems and detail the Super-Nyquist Wavefront Control (SNWC) method, which allows wavefront errors to be controlled beyond the nominal control region of the DM. Our simulations have demonstrated that, with SNWC, raw contrasts of at least 5 × 10{sup −9} in a 10% bandwidth are possible.« less

Simulation of a method to directly image exoplanets around multiple stars systems

NASA Astrophysics Data System (ADS)

Thomas, Sandrine J.; Bendek, Eduardo; Belikov, Ruslan

2014-08-01

Direct imaging of extra-solar planets has now become a reality, especially with the deployment and commissioning of the first generation of specialized ground-based instruments such as the GPI, SPHERE, P1640 and SCExAO. These systems will allow detection of planets 107 times fainter than their host star. For space- based missions, such as EXCEDE, EXO-C, EXO-S, WFIRST/AFTA, different teams have shown in laboratories contrasts reaching 10-10 within a few diffraction limits from the star using a combination of a coronagraph to suppress light coming from the host star and a wavefront control system. These demonstrations use a de- formable mirror (DM) to remove residual starlight (speckles) created by the imperfections of telescope. However, all these current and future systems focus on detecting faint planets around a single host star or unresolved bi- naries/multiples, while several targets or planet candidates are located around nearby binary stars such as our neighbor star Alpha Centauri. Until now, it has been thought that removing the light of a companion star is impossible with current technology, excluding binary star systems from target lists of direct imaging missions. Direct imaging around binaries/multiple systems at a level of contrast allowing Earth-like planet detection is challenging because the region of interest, where a dark zone is essential, is contaminated by the light coming from the hosts star companion. We propose a method to simultaneously correct aberrations and diffraction of light coming from the target star as well as its companion star in order to reveal planets orbiting the target star. This method works even if the companion star is outside the control region of the DM (beyond its half-Nyquist frequency), by taking advantage of aliasing effects.

Reduced Diversity of Life around Proxima Centauri and TRAPPIST-1

NASA Astrophysics Data System (ADS)

Lingam, Manasvi; Loeb, Abraham

2017-09-01

The recent discovery of potentially habitable exoplanets around Proxima Centauri and TRAPPIST-1 has attracted much attention due to their potential for hosting life. We delineate a simple model that accurately describes the evolution of biological diversity on Earth. Combining this model with constraints on atmospheric erosion and the maximal evolutionary timescale arising from the star’s lifetime, we arrive at two striking conclusions: (I) Earth-analogs orbiting low-mass M-dwarfs are unlikely to be inhabited, and (II) K-dwarfs and some G-type stars are potentially capable of hosting more complex biospheres than the Earth. Hence, future searches for biosignatures may have higher chances of success when targeting planets around K-dwarf stars.

A coordinated X-ray, optical, and microwave study of the flare star Proxima Centauri

NASA Technical Reports Server (NTRS)

Haisch, B. M.; Linsky, J. L.; Slee, O. B.; Hearn, D. R.; Walker, A. R.; Rydgren, A. E.; Nicolson, G. D.

1978-01-01

Results are reported for a three-day coordinated observing program to monitor the flare star Proxima Centauri in the X-ray, optical, and radio spectrum. During this interval 30 optical flares and 12 possible radio bursts were observed. The SAS 3 X-ray satellite made no X-ray detections. An upper limit of 0.08 on the X-ray/optical luminosity ratio is derived for the brightest optical flare. The most sensitive of the radio telescopes failed to detect 6-cm emission during one major and three minor optical flares, and on this basis an upper limit on the flare radio emission (1 hundred-thousandth of the optimal luminosity) is derived.

Exploring the climate of Proxima B with the Met Office Unified Model

NASA Astrophysics Data System (ADS)

Boutle, Ian A.; Mayne, Nathan J.; Drummond, Benjamin; Manners, James; Goyal, Jayesh; Hugo Lambert, F.; Acreman, David M.; Earnshaw, Paul D.

2017-05-01

We present results of simulations of the climate of the newly discovered planet Proxima Centauri B, performed using the Met Office Unified Model (UM). We examine the responses of both an "Earth-like" atmosphere and simplified nitrogen and trace carbon dioxide atmosphere to the radiation likely received by Proxima Centauri B. Additionally, we explore the effects of orbital eccentricity on the planetary conditions using a range of eccentricities guided by the observational constraints. Overall, our results are in agreement with previous studies in suggesting Proxima Centauri B may well have surface temperatures conducive to the presence of liquid water. Moreover, we have expanded the parameter regime over which the planet may support liquid water to higher values of eccentricity (≳0.1) and lower incident fluxes (881.7 W m-2) than previous work. This increased parameter space arises because of the low sensitivity of the planet to changes in stellar flux, a consequence of the stellar spectrum and orbital configuration. However, we also find interesting differences from previous simulations, such as cooler mean surface temperatures for the tidally-locked case. Finally, we have produced high-resolution planetary emission and reflectance spectra, and highlight signatures of gases vital to the evolution of complex life on Earth (oxygen, ozone and carbon dioxide).

The Habitability of Proxima Centauri b: Environmental States and Observational Discriminants.

PubMed

Meadows, Victoria S; Arney, Giada N; Schwieterman, Edward W; Lustig-Yaeger, Jacob; Lincowski, Andrew P; Robinson, Tyler; Domagal-Goldman, Shawn D; Deitrick, Russell; Barnes, Rory K; Fleming, David P; Luger, Rodrigo; Driscoll, Peter E; Quinn, Thomas R; Crisp, David

2018-02-01

Proxima Centauri b provides an unprecedented opportunity to understand the evolution and nature of terrestrial planets orbiting M dwarfs. Although Proxima Cen b orbits within its star's habitable zone, multiple plausible evolutionary paths could have generated different environments that may or may not be habitable. Here, we use 1-D coupled climate-photochemical models to generate self-consistent atmospheres for several evolutionary scenarios, including high-O 2 , high-CO 2 , and more Earth-like atmospheres, with both oxic and anoxic compositions. We show that these modeled environments can be habitable or uninhabitable at Proxima Cen b's position in the habitable zone. We use radiative transfer models to generate synthetic spectra and thermal phase curves for these simulated environments, and use instrument models to explore our ability to discriminate between possible planetary states. These results are applicable not only to Proxima Cen b but to other terrestrial planets orbiting M dwarfs. Thermal phase curves may provide the first constraint on the existence of an atmosphere. We find that James Webb Space Telescope (JWST) observations longward of 10 μm could characterize atmospheric heat transport and molecular composition. Detection of ocean glint is unlikely with JWST but may be within the reach of larger-aperture telescopes. Direct imaging spectra may detect O 4 absorption, which is diagnostic of massive water loss and O 2 retention, rather than a photosynthetic biosphere. Similarly, strong CO 2 and CO bands at wavelengths shortward of 2.5 μm would indicate a CO 2 -dominated atmosphere. If the planet is habitable and volatile-rich, direct imaging will be the best means of detecting habitability. Earth-like planets with microbial biospheres may be identified by the presence of CH 4 -which has a longer atmospheric lifetime under Proxima Centauri's incident UV-and either photosynthetically produced O 2 or a hydrocarbon haze layer. Key Words: Planetary habitability and biosignatures-Planetary atmospheres-Exoplanets-Spectroscopic biosignatures-Planetary science-Proxima Centauri b. Astrobiology 18, 133-189.

The Habitability of Proxima Centauri b: Environmental States and Observational Discriminants

PubMed Central

Arney, Giada N.; Schwieterman, Edward W.; Lustig-Yaeger, Jacob; Lincowski, Andrew P.; Robinson, Tyler; Domagal-Goldman, Shawn D.; Deitrick, Russell; Barnes, Rory K.; Fleming, David P.; Luger, Rodrigo; Driscoll, Peter E.; Quinn, Thomas R.; Crisp, David

2018-01-01

Abstract Proxima Centauri b provides an unprecedented opportunity to understand the evolution and nature of terrestrial planets orbiting M dwarfs. Although Proxima Cen b orbits within its star's habitable zone, multiple plausible evolutionary paths could have generated different environments that may or may not be habitable. Here, we use 1-D coupled climate-photochemical models to generate self-consistent atmospheres for several evolutionary scenarios, including high-O2, high-CO2, and more Earth-like atmospheres, with both oxic and anoxic compositions. We show that these modeled environments can be habitable or uninhabitable at Proxima Cen b's position in the habitable zone. We use radiative transfer models to generate synthetic spectra and thermal phase curves for these simulated environments, and use instrument models to explore our ability to discriminate between possible planetary states. These results are applicable not only to Proxima Cen b but to other terrestrial planets orbiting M dwarfs. Thermal phase curves may provide the first constraint on the existence of an atmosphere. We find that James Webb Space Telescope (JWST) observations longward of 10 μm could characterize atmospheric heat transport and molecular composition. Detection of ocean glint is unlikely with JWST but may be within the reach of larger-aperture telescopes. Direct imaging spectra may detect O4 absorption, which is diagnostic of massive water loss and O2 retention, rather than a photosynthetic biosphere. Similarly, strong CO2 and CO bands at wavelengths shortward of 2.5 μm would indicate a CO2-dominated atmosphere. If the planet is habitable and volatile-rich, direct imaging will be the best means of detecting habitability. Earth-like planets with microbial biospheres may be identified by the presence of CH4—which has a longer atmospheric lifetime under Proxima Centauri's incident UV—and either photosynthetically produced O2 or a hydrocarbon haze layer. Key Words: Planetary habitability and biosignatures—Planetary atmospheres—Exoplanets—Spectroscopic biosignatures—Planetary science—Proxima Centauri b. Astrobiology 18, 133–189. PMID:29431479

Project Icarus: The First Unmanned Interstellar Mission - Robotic Expansion and Technological Growth

NASA Astrophysics Data System (ADS)

Long, K. F.

This paper discusses the important role of `disruptive technology' in altering the assessment on when the first unmanned interstellar probe mission is possible. Historical estimates suggest that such a mission is likely possible in the 23rd or 24th century. This paper argues that if such assessments also consider the role of high-growth exponential technology trends then in fact the first unmanned mission may be possible much earlier. The case study of a 100 year flyby space probe mission to Alpha Centauri 4.3 light years distance is examined, with an ideal cruise speed of 2,700 AU/year. Starting from an assumed mission capacity of 5 AU/year in 2020 a simple assessment shows that assuming a greater than ~8% technology growth annually in mission capacity (as measured by the attainment of cruise speed) it may be possible to launch an interstellar probe by around the year 2100. This depends upon significant and sustained science and technology research investment being made, particularly into space propulsion engineering in the near-term. This paper is a submission of the Project Icarus Study Group.

Terrestrial Planet Formation in Binary Star Systems

NASA Technical Reports Server (NTRS)

Lissauer, J. J.; Quintana, E. V.; Adams, F. C.; Chambers, J. E.

2006-01-01

Most stars reside in binary/multiple star systems; however, previous models of planet formation have studied growth of bodies orbiting an isolated single star. Disk material has been observed around one or both components of various young close binary star systems. If planets form at the right places within such disks, they can remain dynamically stable for very long times. We have simulated the late stages of growth of terrestrial planets in both circumbinary disks around 'close' binary star systems with stellar separations ($a_B$) in the range 0.05 AU $\\le a_B \\le$ 0.4 AU and binary eccentricities in the range $0 \\le e \\le 0.8$ and circumstellar disks around individual stars with binary separations of tens of AU. The initial disk of planetary embryos is the same as that used for simulating the late stages of terrestrial planet growth within our Solar System and around individual stars in the Alpha Centauri system (Quintana et al. 2002, A.J., 576, 982); giant planets analogous to Jupiter and Saturn are included if their orbits are stable. The planetary systems formed around close binaries with stellar apastron distances less than or equal to 0.2 AU with small stellar eccentricities are very similar to those formed in the Sun-Jupiter-Saturn, whereas planetary systems formed around binaries with larger maximum separations tend to be sparser, with fewer planets, especially interior to 1 AU. Likewise, when the binary periastron exceeds 10 AU, terrestrial planets can form over essentially the entire range of orbits allowed for single stars with Jupiter-like planets, although fewer terrestrial planets tend to form within high eccentricity binary systems. As the binary periastron decreases, the radial extent of the terrestrial planet systems is reduced accordingly. When the periastron is 5 AU, the formation of Earth-like planets near 1 AU is compromised.

Deceleration of High-velocity Interstellar Photon Sails into Bound Orbits at α Centauri

NASA Astrophysics Data System (ADS)

Heller, René; Hippke, Michael

2017-02-01

At a distance of about 4.22 ly, it would take about 100,000 years for humans to visit our closest stellar neighbor Proxima Centauri using modern chemical thrusters. New technologies are now being developed that involve high-power lasers firing at 1 gram solar sails in near-Earth orbits, accelerating them to 20% the speed of light (c) within minutes. Although such an interstellar probe could reach Proxima 20 years after launch, without propellant to slow it down it would traverse the system within hours. Here we demonstrate how the stellar photon pressures of the stellar triple α Cen A, B, and C (Proxima) can be used together with gravity assists to decelerate incoming solar sails from Earth. The maximum injection speed at α Cen A to park a sail with a mass-to-surface ratio (σ) similar to graphene (7.6 × 10-4 gram m-2) in orbit around Proxima is about 13,800 km s-1 (4.6% c), implying travel times from Earth to α Cen A and B of about 95 years and another 46 years (with a residual velocity of 1280 km s-1) to Proxima. The size of such a low-σ sail required to carry a payload of 10 grams is about 105 m2 = (316 m)2. Such a sail could use solar photons instead of an expensive laser system to gain interstellar velocities at departure. Photogravitational assists allow visits of three stellar systems and an Earth-sized potentially habitable planet in one shot, promising extremely high scientific yields.

Fusion energy for space missions in the 21st century: Executive summary

NASA Technical Reports Server (NTRS)

Schulze, Norman R.

1991-01-01

Future space missions were hypothesized and analyzed, and the energy source of their accomplishment investigated. The missions included manned Mars, scientific outposts to and robotic sample return missions from the outer planets and asteroids, as well as fly-by and rendezvous missions with the Oort Cloud and the nearest star, Alpha Centauri. Space system parametric requirements and operational features were established. The energy means for accomplishing missions where delta v requirements range from 90 km/sec to 30,000 km/sec (High Energy Space Mission) were investigated. The need to develop a power space of this magnitude is a key issue to address if the U.S. civil space program is to continue to advance as mandated by the National Space Policy. Potential energy options which could provide the propulsion and electrical power system and operational requirements were reviewed and evaluated. Fusion energy was considered to be the preferred option and was analyzed in depth. Candidate fusion fuels were evaluated based upon the energy output and neutron flux. Additionally, fusion energy can offer significant safety, environmental, economic, and operational advantages. Reactors exhibiting a highly efficient use of magnetic fields for space use while at the same time offering efficient coupling to an exhaust propellant or to a direct energy convertor for efficient electrical production were examined. Near term approaches were identified. A strategy that will produce fusion powered vehicles as part of the space transportation infrastructure was developed. Space program resources must be directed toward this issue as a matter of the top policy priority.

How Dusty Is Alpha Centauri? Excess or Non-excess over the Infrared Photospheres of Main-sequence Stars

NASA Technical Reports Server (NTRS)

Wiegert, J.; Liseau, R.; Thebault, P.; Olofsson, G.; Mora, A.; Bryden, G.; Marshall, J. P.; Eiroa, C.; Montesinos, B.; Ardila, D.;

THE HOT R CORONAE BOREALIS STAR DY CENTAURI IS A BINARY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kameswara Rao, N.; Lambert, David L.; McArthur, Barbara

2012-11-20

The remarkable hot R Coronae Borealis (RCB) star DY Cen is revealed to be the first and only binary system to be found among the RCB stars and their likely relatives, including the extreme helium stars and the hydrogen-deficient carbon stars. Radial velocity determinations from 1982 to 2010 have shown that DY Cen is a single-lined spectroscopic binary in an eccentric orbit with a period of 39.67 days. It is also one of the hottest and most H-rich member of the class of RCB stars. The system may have evolved from a common envelope to its current form.

Deceleration of High-velocity Interstellar Photon Sails into Bound Orbits at α Centauri

DOE Office of Scientific and Technical Information (OSTI.GOV)

Heller, René; Hippke, Michael, E-mail: heller@mps.mpg.de, E-mail: hippke@ifda.eu

At a distance of about 4.22 ly, it would take about 100,000 years for humans to visit our closest stellar neighbor Proxima Centauri using modern chemical thrusters. New technologies are now being developed that involve high-power lasers firing at 1 gram solar sails in near-Earth orbits, accelerating them to 20% the speed of light ( c ) within minutes. Although such an interstellar probe could reach Proxima 20 years after launch, without propellant to slow it down it would traverse the system within hours. Here we demonstrate how the stellar photon pressures of the stellar triple α Cen A, B,more » and C (Proxima) can be used together with gravity assists to decelerate incoming solar sails from Earth. The maximum injection speed at α Cen A to park a sail with a mass-to-surface ratio ( σ ) similar to graphene (7.6 × 10{sup −4} gram m{sup −2}) in orbit around Proxima is about 13,800 km s{sup −1} (4.6% c ), implying travel times from Earth to α Cen A and B of about 95 years and another 46 years (with a residual velocity of 1280 km s{sup −1}) to Proxima. The size of such a low- σ sail required to carry a payload of 10 grams is about 10{sup 5} m{sup 2} = (316 m){sup 2}. Such a sail could use solar photons instead of an expensive laser system to gain interstellar velocities at departure. Photogravitational assists allow visits of three stellar systems and an Earth-sized potentially habitable planet in one shot, promising extremely high scientific yields.« less

Pulsating stars in ω Centauri. Near-IR properties and period-luminosity relations

NASA Astrophysics Data System (ADS)

Navarrete, Camila; Catelan, Márcio; Contreras Ramos, Rodrigo; Alonso-García, Javier; Gran, Felipe; Dékány, István; Minniti, Dante

2017-09-01

ω Centauri (NGC 5139) contains many variable stars of different types, including the pulsating type II Cepheids, RR Lyrae and SX Phoenicis stars. We carried out a deep, wide-field, near-infrared (IR) variability survey of ω Cen, using the VISTA telescope. We assembled an unprecedented homogeneous and complete J and KS near-IR catalog of variable stars in the field of ω Cen. In this paper we compare optical and near-IR light curves of RR Lyrae stars, emphasizing the main differences. Moreover, we discuss the ability of near-IR observations to detect SX Phoenicis stars given the fact that the amplitudes are much smaller in these bands compared to the optical. Finally, we consider the case in which all the pulsating stars in the three different variability types follow a single period-luminosity relation in the near-IR bands.

Prospects for Characterizing the Atmosphere of Proxima Centauri b

NASA Astrophysics Data System (ADS)

Kreidberg, Laura; Loeb, Abraham

2016-11-01

The newly detected Earth-mass planet in the habitable zone of Proxima Centauri could potentially host life—if it has an atmosphere that supports surface liquid water. We show that thermal phase curve observations with the James Webb Space Telescope (JWST) from 5-12 μm can be used to test for the existence of such an atmosphere. We predict the thermal variation for a bare rock versus a planet with 35% heat redistribution to the nightside and show that a JWST phase curve measurement can distinguish between these cases at 4σ confidence, assuming photon-limited precision. We also consider the case of an Earth-like atmosphere, and find that the 9.8 μm ozone band could be detected with longer integration times (a few months). We conclude that JWST observations have the potential to put the first constraints on the possibility of life around the the solar system’s nearest star.

Fermi-LAT Gamma-Ray Detections of Classical Novae V1369 Centauri 2013 and V5668 Sagittarii 2015

NASA Astrophysics Data System (ADS)

Cheung, C. C.; Jean, P.; Shore, S. N.; Stawarz, Ł.; Corbet, R. H. D.; Knödlseder, J.; Starrfield, S.; Wood, D. L.; Desiante, R.; Longo, F.; Pivato, G.; Wood, K. S.

2016-08-01

We report the Fermi Large Area Telescope (LAT) detections of high-energy (>100 MeV) γ-ray emission from two recent optically bright classical novae, V1369 Centauri 2013 and V5668 Sagittarii 2015. At early times, Fermi target-of-opportunity observations prompted by their optical discoveries provided enhanced LAT exposure that enabled the detections of γ-ray onsets beginning ˜2 days after their first optical peaks. Significant γ-ray emission was found extending to 39-55 days after their initial LAT detections, with systematically fainter and longer-duration emission compared to previous γ-ray-detected classical novae. These novae were distinguished by multiple bright optical peaks that encompassed the time spans of the observed γ-rays. The γ-ray light curves and spectra of the two novae are presented along with representative hadronic and leptonic models, and comparisons with other novae detected by the LAT are discussed.

THE SPACE WEATHER OF PROXIMA CENTAURI b

DOE Office of Scientific and Technical Information (OSTI.GOV)

Garraffo, C.; Drake, J. J.; Cohen, O., E-mail: cgaraffo@cfa.harvard.edu

A planet orbiting in the “habitable zone” of our closest neighboring star, Proxima Centauri, has recently been discovered, and the next natural question is whether or not Proxima b is “habitable.” Stellar winds are likely a source of atmospheric erosion that could be particularly severe in the case of M dwarf habitable zone planets that reside close to their parent star. Here, we study the stellar wind conditions that Proxima b experiences over its orbit. We construct 3D MHD models of the wind and magnetic field around Proxima Centauri using a surface magnetic field map for a star of themore » same spectral type and scaled to match the observed ∼600 G surface magnetic field strength of Proxima. We examine the wind conditions and dynamic pressure over different plausible orbits that sample the constrained parameters of the orbit of Proxima b. For all the parameter space explored, the planet is subject to stellar wind pressures of more than 2000 times those experienced by Earth from the solar wind. During an orbit, Proxima b is also subject to pressure changes of 1–3 orders of magnitude on timescales of a day. Its magnetopause standoff distance consequently undergoes sudden and periodic changes by a factor of 2–5. Proxima b will traverse the interplanetary current sheet twice each orbit, and likely crosses into regions of subsonic wind quite frequently. These effects should be taken into account in any physically realistic assessment or prediction of its atmospheric reservoir, characteristics, and loss.« less

Baby Solar System

NASA Technical Reports Server (NTRS)

Currie, Thayne; Grady, Carol

2012-01-01

What did our solar system look like in its infancy,...... when the planets were forming? We cannot travel back in time to take an image of the early solar system, but in principle we can have the next best thing: images of infant planetary systems around Sun-like stars with ages of 1 to 5 million years, the time we think it took for the giant planets to form. Infant exoplanetary systems are critically important because they can help us understand how our solar system fits within the context of planet formation in general. More than 80% of stars are born with gas- and dust-rich disks, and thus have the potential to form planets. Through many methods we have identified more than 760 planetary systems around middle-aged stars like the Sun, but many of these have architectures that look nothing like our solar system. Young planetary systems are important missing links between various endpoints and may help us understand how and when these differences emerge. Well-known star-forming regions in Taurus, Scorpius. and Orion contain stars that could have infant planetary systems. But these stars are much more distant than our nearest neighbors such as Alpha Centauri or Sirius, making it extremely challenging to produce clear images of systems that can reveal signs of recent planet formation, let alone reveal the planets themselves. Recently, a star with the unassuming name LkCa 15 may have given us our first detailed "baby picture" of a young planetary system similar to our solar system. Located about 450 light-years away in the Taurus starforming region. LkCa 15 has a mass comparable to the Sun (0.97 solar mass) and an age of l to 5 million years, comparable to the time at which Saturn and perhaps Jupiter formed. The star is surrounded by a gas-rich disk similar in structure to the one in our solar system from which the planets formed. With new technologies and observing strategies, we have confirmed suspicions that LkCa 15's disk harbors a young planetary system.

Flare activity and photospheric analysis of Proxima Centauri

NASA Astrophysics Data System (ADS)

Pavlenko, Y.; Suárez Mascareño, A.; Rebolo, R.; Lodieu, N.; Béjar, V. J. S.; González Hernández, J. I.

2017-10-01

Context. We present the analysis of emission lines in high-resolution optical spectra of the planet-host star Proxima Centauri (Proxima) classified as a M5.5V. Aims: We carry out a detailed analysis of the observed spectra to get a better understanding of the physical conditions of the atmosphere of this star. Methods: We identify the emission lines in a series of 147 high-resolution optical spectra of the star at different levels of activity and compare them with the synthetic spectra computed over a wide spectral range. Results: Our synthetic spectra computed with the PHOENIX 2900/5.0/0.0 model atmosphere fits the observed spectral energy distribution from optical to near-infrared quite well. However, modelling strong atomic lines in the blue spectrum (3900-4200 Å) requires implementing additional opacity. We show that high-temperature layers in Proxima Centauri consist of at least three emitting parts: a) a stellar chromosphere where numerous emission lines form; we suggest that some emission cores of strong absorption lines of metals form there; b) flare regions above the chromosphere, where hydrogen Balmer lines up to high transition levels (10-2) form; and c) a stellar wind component with Vr = -30 km s-1 seen in some Balmer lines as blueshifted emission lines. We believe that the observed He line at 4026 Å in emission can be formed in that very hot region. Conclusions: We show that the real structure of the atmosphere of Proxima is rather complicated. The photosphere of the star is best fit by a normal M5 dwarf spectrum. On the other hand, emission lines form in the chromosphere, flare regions, and extended hot envelope. The movies are available at http://www.aanda.org

Fourier Analysis of First-Overtone RR Lyrae Variables in the LMC

NASA Astrophysics Data System (ADS)

Clement, C. M.; Muzzin, A. V.; Rowe, J. F.; MACHO Collaboration

2002-05-01

Simon's (1989, ApJ, 343, L17) Fourier decomposition technique has been applied to the V magnitudes of the first-overtone RR Lyrae (RR1) variables in 16 LMC fields observed by the MACHO collaboration. The Fourier coefficients R21 and φ 31 derived for these stars have been compared with the coefficients of RR1 variables in the galactic globular clusters Omega Centauri, M2, M3, M5, M68, M107 (NGC 6171) and NGC 6441. Our analysis indicates that the majority of the LMC RR1 variables have coefficients similar to those in the Oosterhoff type I (OoI) clusters M3 and M5 and to the OoI variables in Omega Centauri. In a study of hydrodynamic pulsation models of first overtone RR Lyrae variables, Simon & Clement (1993, ApJ, 410, 526) found that the Fourier phase parameter φ 31 depends essentially on mass and luminosity. From this, we conclude that the masses and luminosities of most of the RR1 variables in the LMC are comparable to those of the OoI RR1 variables in Omega Centauri, M3 and M5, a fact that should be considered when RR Lyrae variables are used for determining the distance to the LMC. The MACHO collaboration includes C. Alcock, R. A. Allsman, D. R. Alves, T. S. Axelrod, A. C. Becker, D. P. Bennet, K. H. Cook, A. J. Drake, K. C. Freeman, M. Geha, K. Griest, M. J. Lehner, S. L. Marshall, D. Minniti, C. A. Nelson, B. A. Peterson, P. Popowski, M. R. Pratt, P. J. Quinn, C. W. Stubbs, W. Sutherland, T. Vandehel and D. L. Welch. This research has been supported in part by the Natural Sciences and Engineering Research Council of Canada.

Detection of a Millimeter Flare from Proxima Centauri

NASA Astrophysics Data System (ADS)

MacGregor, Meredith A.; Weinberger, Alycia J.; Wilner, David J.; Kowalski, Adam F.; Cranmer, Steven R.

2018-03-01

We present new analyses of ALMA 12 m and Atacama Compact Array (ACA) observations at 233 GHz (1.3 mm) of the Proxima Centauri system with sensitivities of 9.5 and 47 μJy beam‑1, respectively, taken from 2017 January 21 through April 25. These analyses reveal that the star underwent a significant flaring event during one of the ACA observations on 2017 March 24. The complete event lasted for approximately 1 minute and reached a peak flux density of 100 ± 4 mJy, nearly a factor of 1000 times brighter than the star’s quiescent emission. At the flare peak, the continuum emission is characterized by a steeply falling spectral index with frequency F ν ∝ ν α with α = ‑1.77 ± 0.45, and a lower limit on the fractional linear polarization of | Q/I| =0.19+/- 0.02. Because the ACA observations do not show any quiescent excess emission, we conclude that there is no need to invoke the presence of a dust belt at 1–4 au. We also posit that the slight excess flux density of 101 ± 9 μJy observed in the 12 m observations, compared to the photospheric flux density of 74 ± 4 μJy extrapolated from infrared wavelengths, may be due to coronal heating from continual smaller flares, as is seen for AU Mic, another nearby well-studied M dwarf flare star. If this is true, then the need for warm dust at ∼0.4 au is also removed.

Near-IR Period-Luminosity Relations for variable stars in ω Centauri

NASA Astrophysics Data System (ADS)

Navarrete, C.; Catelan, M.; Contreras Ramos, R.; Gran, F.; Alonso-García, J.; Dékány, I.

2014-10-01

We report on an extensive time-series study of the globular cluster ω Centauri (NGC 5139), obtained in the framework of the VVV Templates project (Catelan et al. 2013, arXiv: 1310.1996). This cluster was chosen for this project due to its large variable star content. A total of 42 and 100 epochs of the cluster in J and K_{S}, respectively, were taken using VIRCAM@VISTA, and PSF photometry was performed to derive light curves for 270 pulsating stars (RRab, RRc, type II Cepheids and SX Phoenicis) with an unprecedented phase coverage in the near-IR. Period-Luminosity (PL) relations in both bands were derived using Fourier fitted magnitudes for RR Lyrae and Type II Cepheids, while weighted-average magnitudes were used for SX Phe stars. Using the PL relation for RRab stars derived by Dékány et al. (2013, ApJ, 776, 19L) in the VISTA K_{S} system, we determine a distance modulus of (m-M)_{0} = 13.78 ± 0.04 mag, in good agreement with Del Principe et al. (2006, ApJ, 652, 362). From Type II Cepheids we derived a value of (m-M)_{0} = 13.67 ± 0.07 mag, similar to what was found by Matsunaga et al. (2006, MNRAS, 370, 1979). For SX Phe stars, we use the derived periods and magnitudes to infer their pulsation modes, and we confirm that at least 12 of them are fundamental-mode pulsators (Olech et al. 2005, MNRAS, 363, 40).

Astrometry of the omega Centauri Hubble Space Telescope Calibration Field

NASA Technical Reports Server (NTRS)

Mighell, Kenneth J.

2000-01-01

Astrometry, on the International Celestial Reference Frame (epoch J2000.0), is presented for the Walker (1994, PASP, 106, 828) stars in the omega Centauri (=NGC 5139=C 1323-1472) Hubble Space Telescope Wide Field/Planetary Camera (WF/PC) calibration field of Harris et al. (1993, AJ, 105, 1196). Harris et al. stars were first identified on a WFPC2 observation of the omega Cen HST calibration field. Relative astrometry of the Walker stars in this field was then obtained using Walker's CCD positions and astrometry derived using the STSDAS METRIC task on the positions of the Harris et al. stars on the WFPC2 observation. Finally, the relative astrometry, which was based on the HST Guide Star Catalog, is placed on the International Celestial Reference Frame with astrometry from the USNO-A2.0 catalog. An ASCII text version of the astrometric data of the Walker stars in the omega Cen HST calibration field is available electronically in the online version of the article.

PROSPECTS FOR CHARACTERIZING THE ATMOSPHERE OF PROXIMA CENTAURI b

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kreidberg, Laura; Loeb, Abraham, E-mail: laura.kreidberg@cfa.harvard.edu

2016-11-20

The newly detected Earth-mass planet in the habitable zone of Proxima Centauri could potentially host life—if it has an atmosphere that supports surface liquid water. We show that thermal phase curve observations with the James Webb Space Telescope ( JWST ) from 5–12 μ m can be used to test for the existence of such an atmosphere. We predict the thermal variation for a bare rock versus a planet with 35% heat redistribution to the nightside and show that a JWST phase curve measurement can distinguish between these cases at 4 σ confidence, assuming photon-limited precision. We also consider themore » case of an Earth-like atmosphere, and find that the 9.8 μ m ozone band could be detected with longer integration times (a few months). We conclude that JWST observations have the potential to put the first constraints on the possibility of life around the the solar system’s nearest star.« less

Fermi-LAT gamma ray detections of classical novae V1369 centauri 2013 and V5668 Sagittarii 2015

DOE PAGES

Cheung, C. C.; Jean, P.; Shore, S. N.; ...

2016-07-27

Here, we report the Fermi Large Area Telescope (LAT) detections of high-energy (>100 MeV) γ-ray emission from two recent optically bright classical novae, V1369 Centauri 2013 and V5668 Sagittarii 2015. Furthermore, at early times, Fermi target-of-opportunity observations prompted by their optical discoveries provided enhanced LAT exposure that enabled the detections of γ-ray onsets beginning ~2 days after their first optical peaks. Significant γ-ray emission was found extending to 39–55 days after their initial LAT detections, with systematically fainter and longer-duration emission compared to previous γ-ray-detected classical novae. These novae were distinguished by multiple bright optical peaks that encompassed the timemore » spans of the observed γ-rays. Finally, we discussed the γ-ray light curves and spectra of the two novae are presented along with representative hadronic and leptonic models, and comparisons with other novae detected by the LAT.« less

Captured by Aliens

NASA Astrophysics Data System (ADS)

Achenbach, Joel

2000-03-01

Captured by Aliens is a long and twisted voyage from science to the supernatural and back again. I hung out in Roswell, N.M., spent time with the Mars Society, met a guy who was figuring out the best way to build a spaceship to go to Alpha Centauri. I visited the set of the X-Files and talked to Mulder and Scully. One day over breakfast I was told by NASA administrator Dan Goldin, We live in a fog, man! He wants the big answers to the big questions. I spent a night in the base of a huge radio telescope in the boondocks of West Virginia, awaiting the signal from the aliens. I was hypnotized in a hotel room by someone who suspected that I'd been abducted by aliens and that this had triggered my interest in the topic. In the last months of his life, I talked to Carl Sagan, who believed that the galaxy riots with intelligent civilizations. He's my hero, for his steadfast adherence to the scientific method. What I found in all this is that the big question that needs immediate attention is not what's out THERE, but what's going on HERE, on Earth, and why we think the way we do, and how we came to be here in the first place.

The Influence of a Substellar Continent on the Climate of a Tidally Locked Exoplanet

NASA Astrophysics Data System (ADS)

Lewis, Neil T.; Lambert, F. Hugo; Boutle, Ian A.; Mayne, Nathan J.; Manners, James; Acreman, David M.

2018-02-01

Previous studies have demonstrated that continental carbon-silicate weathering is important to the continued habitability of a terrestrial planet. Despite this, few studies have considered the influence of land on the climate of a tidally locked planet. In this work we use the Met Office Unified Model, coupled to a land-surface model, to investigate the climate effects of a continent located at the substellar point. We choose to use the orbital and planetary parameters of Proxima Centauri B as a template, to allow comparison with the work of others. A region of the surface where T s > 273.15 K is always retained, and previous conclusions on the habitability of Proxima Centauri B remain intact. We find that substellar land causes global cooling and increases day–night temperature contrasts by limiting heat redistribution. Furthermore, we find that substellar land is able to introduce a regime change in the atmospheric circulation. Specifically, when a continent offset to the east of the substellar point is introduced, we observe the formation of two mid-latitude counterrotating jets, and a substantially weakened equatorial superrotating jet.

Solar lens mission concept for interstellar exploration

NASA Astrophysics Data System (ADS)

Brashears, Travis; Lubin, Philip; Turyshev, Slava; Shao, Michael; Zhang, Qicheng

2015-09-01

The long standing approach to space travel has been to incorporate massive on-board electronics, probes and propellants to achieve space exploration. This approach has led to many great achievements in science, but will never help to explore the interstellar medium. Fortunately, a paradigm shift is upon us in how a spacecraft is constructed and propelled. This paper describes a mission concept to get to our Sun's Gravity Lens at 550AU in less than 10 years. It will be done by using DE-STAR, a scalable solar-powered phased-array laser in Earth Orbit, as a directed energy photon drive of low-mass wafersats. [1] [2] [3] [4] [5] With recent technologies a complete mission can be placed on a wafer including, power from an embedded radio nuclear thermal generator (RTG), PV, laser communications, imaging, photon thrusters for attitude control and other sensors. As one example, a futuristic 200 MW laser array consisting of 1 - 10 kw meter scale sub elements with a 100m baseline can propel a 10 gram wafer scale spacecraft with a 3m laser sail to 60AU/Year. Directed energy propulsion of low-mass spacecraft gives us an opportunity to capture images of Alpha Centauri and its planets, detailed imaging of the cosmic microwave background, set up interstellar communications by using gravity lenses around nearby stars to boost signals from interstellar probes, and much more. This system offers a very large range of missions allowing hundreds of wafer scale payload launches per day to reach this cosmological data reservoir. Directed Energy Propulsion is the only current technology that can provide a near-term path to utilize our Sun's Gravity Lens.

VizieR Online Data Catalog: Omega Cen candidates RAVE-selected (Fernandez-Trincado+, 2015)

NASA Astrophysics Data System (ADS)

Fernandez-Trincado, J. G.; Robin, A. C.; Vieira, K.; Moreno, E.; Bienayme, O.; Reyle, C.; Valenzuela, O.; Pichardo, B.; Robles-Valdez, F.; Martins, A. M. M.

2015-11-01

The sample was selected from the RAVE DR4 catalog (Kordopatis et al., 2013, Cat. III/272), which provides accurate radial velocities with typical errors of σRV~2km/s, and distances and individual abundances with errors of about 10-20%, determined for approximately 390000 relatively bright stars (9mag20 (algo_conv=0 was required, indicating that the pipeline converges, see Kordopatis et al., 2013, Cat. III/272). This cut allowed us to obtain precise radial velocity measurements, typically σRV<2km/s, in order to constraint the full space motion. The metallicity [Fe/H] distribution for giant stars within Omega Centauri spans more than a magnitude order, from -2.2dex<[Fe/H]<-0.7dex (Johnson & Pilachowski, 2010, Cat. J/ApJ/722/1373), therefore we allowed stars in our sample to be in this range of metallicity. (1 data file).

Centaurus

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

(the Centaur; abbrev. Cen, gen. Centauri; area 1060 sq. deg.) A southern constellation which lies between Vela and Lupus, and surrounds Crux on three sides. It culminates at midnight in early April. Its origin dates back at least to ancient Greece, where it was identified with Chiron in Greek mythology. The brightest stars of Centaurus were cataloged by Ptolemy (c. AD 100-175) in the Almagest....

A Search for Extreme Horizontal Branch Stars in the General Field Population (Abstract)

NASA Astrophysics Data System (ADS)

Walker, D.; Albrow, M.

2014-12-01

(Abstract only) The study of pulsating Extreme Horizontal Branch (EHB) stars in globular clusters is a new field of stellar research. The initial discovery of three rapidly pulsating EHB stars in w Centauri was announced at the Fourth Meeting on Hot Subdwarfs and Related Objects held in Shanghai in July 2009. A fourth sdB pulsator was discovered in the remaining photometry data soon afterwards; all were discovered in data obtained by the New Techology Telescope. In March 2013, the Space Telescope Imaging Spectrograph (STIS) was utilized on five consecutive orbits to obtain far-UV imagery of NGC 2808’s core, revealing six sdB pulsators with periods 85 to 149 seconds and UV amplitudes from 2.0 to 6.8%. To date (April 2014), these ten EHB pulsators in w Centauri and NGC 2808 form a unique class of EHB variable closely clustered around Teff ~ 50,000 K. This talk describes an initial candidate search for EHB rapidly pulsating sdB stars in the general galactic field population. The search was conducted with the 1-m McLellan telescope at the Mt. John University Observatory (MJUO) at Lake Tekapo, New Zealand. Observations were conducted utilizing a special high speed f/8 frame-transfer camera called the Puoko-nui. The candidate set of stars was taken from the Edinburgh-Cape Blue Object Survey based on the selection criteria of a (B-V) value of -0.32 to -0.36, corresponding to the desired temperature range Teff ranging from 40,000 to 64,000 K. The objective of this search was to determine whether smaller size telescopes could identify promising sets of candidate sdB pulsators which could be followed up with larger professional systems.

A Deep X-ray Survey of the Globular Cluster Omega Centauri

NASA Astrophysics Data System (ADS)

Henleywillis, Simon; Cool, Adrienne M.; Haggard, Daryl; Heinke, Craig; Callanan, Paul; Zhao, Yue

2018-03-01

We identify 233 X-ray sources, of which 95 are new, in a 222 ks exposure of Omega Centauri with the Chandra X-ray Observatory's ACIS-I detector. The limiting unabsorbed flux in the core is fX(0.5-6.0 keV) ≃ 3×10-16 erg s-1 cm-2 (Lx ≃ 1×1030 erg s-1 at 5.2 kpc). We estimate that ˜60 ± 20 of these are cluster members, of which ˜30 lie within the core (rc = 155 arcsec), and another ˜30 between 1-2 core radii. We identify four new optical counterparts, for a total of 45 likely identifications. Probable cluster members include 18 cataclysmic variables (CVs) and CV candidates, one quiescent low-mass X-ray binary, four variable stars, and five stars that are either associated with ω Cen's anomalous red giant branch, or are sub-subgiants. We estimate that the cluster contains 40 ± 10 CVs with Lx > 1031 erg s-1, confirming that CVs are underabundant in ω Cen relative to the field. Intrinsic absorption is required to fit X-ray spectra of six of the nine brightest CVs, suggesting magnetic CVs, or high-inclination systems. Though no radio millisecond pulsars (MSPs) are currently known in ω Cen, more than 30 unidentified sources have luminosities and X-ray colours like those of MSPs found in other globular clusters; these could be responsible for the Fermi-detected gamma-ray emission from the cluster. Finally, we identify a CH star as the counterpart to the second-brightest X-ray source in the cluster and argue that it is a symbiotic star. This is the first such giant/white dwarf binary to be identified in a globular cluster.

Solar System, in Perspective

NASA Image and Video Library

2014-03-24

This artist's concept puts solar system distances in perspective. The scale bar is in astronomical units, with each set distance beyond 1 AU representing 10 times the previous distance. One AU is the distance from the sun to the Earth, which is about 93 million miles or 150 million kilometers. Neptune, the most distant planet from the sun, is about 30 AU. Informally, the term "solar system" is often used to mean the space out to the last planet. Scientific consensus, however, says the solar system goes out to the Oort Cloud, the source of the comets that swing by our sun on long time scales. Beyond the outer edge of the Oort Cloud, the gravity of other stars begins to dominate that of the sun. The inner edge of the main part of the Oort Cloud could be as close as 1,000 AU from our sun. The outer edge is estimated to be around 100,000 AU. NASA's Voyager 1, humankind's most distant spacecraft, is around 125 AU. Scientists believe it entered interstellar space, or the space between stars, on Aug. 25, 2012. Much of interstellar space is actually inside our solar system. It will take about 300 years for Voyager 1 to reach the inner edge of the Oort Cloud and possibly about 30,000 years to fly beyond it. Alpha Centauri is currently the closest star to our solar system. But, in 40,000 years, Voyager 1 will be closer to the star AC +79 3888 than to our own sun. AC +79 3888 is actually traveling faster toward Voyager 1 than the spacecraft is traveling toward it. The Voyager spacecraft were built and continue to be operated by NASA's Jet Propulsion Laboratory, in Pasadena, Calif. Caltech manages JPL for NASA. The Voyager missions are a part of NASA's Heliophysics System Observatory, sponsored by the Heliophysics Division of the Science Mission Directorate at NASA Headquarters in Washington. For more information about Voyager, visit: www.nasa.gov/voyager and voyager.jpl.nasa.gov . Image credit: NASA/JPL-Caltech NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Stratosphere circulation on tidally locked ExoEarths

NASA Astrophysics Data System (ADS)

Carone, L.; Keppens, R.; Decin, L.; Henning, Th.

2018-02-01

Stratosphere circulation is important to interpret abundances of photochemically produced compounds like ozone which we aim to observe to assess habitability of exoplanets. We thus investigate a tidally locked ExoEarth scenario for TRAPPIST-1b, TRAPPIST-1d, Proxima Centauri b and GJ 667 C f with a simplified 3D atmosphere model and for different stratospheric wind breaking assumptions.

Physical state of interstellar atoms. [from Copernicus satellite UV data

NASA Technical Reports Server (NTRS)

York, D. G.

1974-01-01

Brief survey of the physical conditions along the lines of sight to reddened and unreddened stars, as determined from Copernicus observation of interstellar lines between 95 and 300 nm. Differences in ionization structure and density between clouds and the local intercloud medium are discussed. Some new data for beta Centauri is used to supplement the previously available data.

MOST Observations of Our Nearest Neighbor: Flares on Proxima Centauri

NASA Astrophysics Data System (ADS)

Davenport, James R. A.; Kipping, David M.; Sasselov, Dimitar; Matthews, Jaymie M.; Cameron, Chris

2016-10-01

We present a study of white-light flares from the active M5.5 dwarf Proxima Centauri using the Canadian microsatellite Microvariability and Oscillations of STars. Using 37.6 days of monitoring data from 2014 to 2015, we have detected 66 individual flare events, the largest number of white-light flares observed to date on Proxima Cen. Flare energies in our sample range from 1029 to 1031.5 erg. The flare rate is lower than that of other classic flare stars of a similar spectral type, such as UV Ceti, which may indicate Proxima Cen had a higher flare rate in its youth. Proxima Cen does have an unusually high flare rate given its slow rotation period, however. Extending the observed power-law occurrence distribution down to 1028 erg, we show that flares with flux amplitudes of 0.5% occur 63 times per day, while superflares with energies of 1033 erg occur ∼8 times per year. Small flares may therefore pose a great difficulty in searches for transits from the recently announced 1.27 M ⊕ Proxima b, while frequent large flares could have significant impact on the planetary atmosphere.

Variability Survey of ω Centauri in the Near-IR: Period-Luminosity Relations

NASA Astrophysics Data System (ADS)

Navarrete, Camila; Catelan, Márcio; Contreras Ramos, Rodrigo; Gran, Felipe; Alonso-García, Javier; Dékány, István

2015-08-01

ω Centauri (NGC 5139) is by far the most massive globular star cluster in the Milky Way, and has even been suggested to be the remnant of a dwarf galaxy. As such, it contains a large number of variable stars of different classes. Here we report on a deep, wide-field, near-infrared variability survey of omega Cen, carried out by our team using ESO's 4.1m VISTA telescope. Our time-series data comprise 42 and 100 epochs in J and Ks, respectively. This unique dataset has allowed us to derive complete light curves for hundreds of variable stars in the cluster, and thereby perform a detailed analysis of the near-infrared period-luminosity (PL) relations for different variability classes, including type II Cepheids, SX Phoenicis, and RR Lyrae stars. In this contribution, in addition to describing our survey and presenting the derived light curves, we present the resulting PL relations for each of these variability classes, including the first calibration of this sort for the SX Phoenicis stars. Based on these relations, we also provide an updated (pulsational) distance modulus for omega Cen, compare with results based on independent techniques, and discuss possible sources of systematic errors.

MOST OBSERVATIONS OF OUR NEAREST NEIGHBOR: FLARES ON PROXIMA CENTAURI

DOE Office of Scientific and Technical Information (OSTI.GOV)

Davenport, James R. A.; Kipping, David M.; Sasselov, Dimitar

2016-10-01

We present a study of white-light flares from the active M5.5 dwarf Proxima Centauri using the Canadian microsatellite Microvariability and Oscillations of STars . Using 37.6 days of monitoring data from 2014 to 2015, we have detected 66 individual flare events, the largest number of white-light flares observed to date on Proxima Cen. Flare energies in our sample range from 10{sup 29} to 10{sup 31.5} erg. The flare rate is lower than that of other classic flare stars of a similar spectral type, such as UV Ceti, which may indicate Proxima Cen had a higher flare rate in its youth.more » Proxima Cen does have an unusually high flare rate given its slow rotation period, however. Extending the observed power-law occurrence distribution down to 10{sup 28} erg, we show that flares with flux amplitudes of 0.5% occur 63 times per day, while superflares with energies of 10{sup 33} erg occur ∼8 times per year. Small flares may therefore pose a great difficulty in searches for transits from the recently announced 1.27 M {sub ⊕} Proxima b, while frequent large flares could have significant impact on the planetary atmosphere.« less

Living With A Red Dwarf: Rotation, Starspots, Activity Cycles, Coronal X-ray Activity And X-uv Irradiances Of Proxima Centauri

NASA Astrophysics Data System (ADS)

Jason, Merritt; Guinan, E.; Engle, S.; Pojmanski, G.

2007-12-01

As part of our Living with a Red Dwarf Program, we have carried out a detailed study of the radiative and plasma properties of the nearby dM5.5e star Proxima Centauri. Proxima Cen is noteworthy as the nearest star to the Sun. Because of its proximity ( 4.3 L.Y.) and membership in the α Cen system, Proxima Cen is an important star to use as a surrogate for solar-aged mid-dM stars. It is relatively bright (V = 11-mag) and has well determined observational and physical properties (MV, Teff, [Fe/H], angular diameter, mass and age). Importantly for our purposes, Proxima Cen has a reliable age of 5.5-6.0 Gyr from its association with the α Cen system in which α Cen A (G2 V) has a reliable isochronal age determination. We have analyzed 5 years of ASAS-3, V-band photometry to search for evidence of short- and long-term variations in brightness that could arise from magnetically related phenomenon (star spots, faculae, and possible UV flares). We also examine its coronal X-ray emission and variations as well as the stars chromospheric and transition regions in the UV from IUE and FUSE observations. The X-UV/optical data are combined and irradiances are calculated for use in extrasolar planet studies. From the photometry we find a rotational modulation of Prot = 83.5 days, in excellent agreement with the earlier HST/FGS study of Benedict et al. (1998). The character of its light variations indicates possible differential rotation as well as a probable long-term activity cycle of 6.9 +/- 0.5 yrs. Although Proxima Cen should be a fully convective star with a different magnetic dynamo (α2) than our Sun (αΩ), its overall magnetic behavior appears to be solar-like. This research is supported by grants from NSF/RUI AST-507536 and NASA Grants NNX06AD386 and NNG04G038G. We are grateful for this support.

VizieR Online Data Catalog: MOST photometry of Proxima (Kipping+, 2017)

NASA Astrophysics Data System (ADS)

Kipping, D. M.; Cameron, C.; Hartman, J. D.; Davenport, J. R. A.; Matthews, J. M.; Sasselov, D.; Rowe, J.; Siverd, R. J.; Chen, J.; Sandford, E.; Bakos, G. A.; Jordan, A.; Bayliss, D.; Henning, T.; Mancini, L.; Penev, K.; Csubry, Z.; Bhatti, W.; da Silva Bento, J.; Guenther, D. B.; Kuschnig, R.; Moffat, A. F. J.; Rucinski, S. M.; Weiss, W. W.

2017-06-01

Microwave and Oscillations of STars (MOST) telescope is a 53kg satellite in low Earth orbit with a 15cm aperture visible band camera (35-750nm). MOST observed Proxima Centauri in 2014 May (beginning on HJD(2000) 2456793.18) for about 12.5 days. MOST again observed Proxima Centauri in 2015 May (starting on HJD(2000) 2457148.54), this time for a total of 31 days. Independent of the MOST observations, Proxima Cen was also monitored by the HATSouth ground-based telescope network. The network consists of six wide-field photometric instruments located at three observatories in the Southern Hemisphere (Las Campanas Observatory [LCO] in Chile, the High Energy Stereoscopic System [HESS] site in Namibia, and Siding Spring Observatory [SSO] in Australia), with two instruments per site. Each instrument consists of four 18cm diameter astrographs and associated 4K*4K backside-illuminated CCD cameras and Sloan r-band filters, placed on a common robotic mount. The four astrographs and cameras together cover a 8.2°*8.2° mosaic field of view at a pixel scale of 3.7''/pixel. Observations of a field containing Proxima Cen were collected as part of the general HATSouth transit survey, with a total of 11071 (this number does not count observations that were rejected as not useful for high-precision photometry, or those that produced large-amplitude outliers in the Proxima Cen light curve) composite 3*80s exposures gathered between 2012 June 14 and 2014 September 20. These include 3430 observations made with the HS-2 unit at LCO, 4630 observations made with the HS-4 unit at the HESS site, and 3011 observations made with the HS-6 unit at the SSO site. Due to weather and other factors, the cadence was nonuniform. The median time difference between consecutive observations in the full time series is 368s. (2 data files).

The Cycles of α Centauri

NASA Astrophysics Data System (ADS)

Ayres, Thomas R.

2009-05-01

The main AB pair of the nearby α Centauri triple system has one of the most extensive X-ray records of any cosmic object, stretching over 30 years. The primary, α Cen A (G2 V), is a near twin of the Sun, with a similarly soft (1-2 MK) corona. The secondary, α Cen B (K1 V), is more active than the Sun, with a generally harder coronal spectrum. Here, more than a decade of spatially resolved measurements from ROSAT, XMM-Newton, and Chandra are compared on a common basis, with careful attention to conversion factors that translate count rates of the different instruments into absolute energy fluxes. For the latter purpose, two epochs of Chandra transmission grating spectra, which fully resolve the binary, were modeled using a differential emission measure formalism. The aggregate time series suggests that α Cen B was near X-ray maximum in the mid-1990s, minimum in the late-1990s, then peaked again in 2004-2005, and more recently has been declining toward another minimum. Meanwhile, α Cen A showed minimal variability 1995-2000, and like the secondary presently is mired in an activity lull (in fact, as seen by XMM-Newton, the primary "fainted" from view in the 2005 time frame). Comparisons between X-ray luminosities in the 0.2-2 keV (6-60 Å) ROSAT "WGACAT" band and a softer counterpart 0.06-1.2 keV (10-200 Å) reinforce the idea that cycle depth is strongly dependent on the energy span of the measurement, and that much of the coronal luminosity of cool-corona objects like the Sun falls at longer wavelengths than are recorded efficiently by contemporary instruments. Consequently, one must be careful in discussing X-ray cycles, their amplitudes, and coronal heating requirements unless one can demonstrate good control over the out-of-band component.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dong, Chuanfei; Lingam, Manasvi; Ma, Yingjuan

We address the important question of whether the newly discovered exoplanet, Proxima Centauri b (PCb), is capable of retaining an atmosphere over long periods of time. This is done by adapting a sophisticated multi-species MHD model originally developed for Venus and Mars and computing the ion escape losses from PCb. The results suggest that the ion escape rates are about two orders of magnitude higher than the terrestrial planets of our Solar system if PCb is unmagnetized. In contrast, if the planet does have an intrinsic dipole magnetic field, the rates are lowered for certain values of the stellar windmore » dynamic pressure, but they are still higher than the observed values for our solar system’s terrestrial planets. These results must be interpreted with due caution since most of the relevant parameters for PCb remain partly or wholly unknown.« less

Exoplanet-induced Radio Emission from M Dwarfs

NASA Astrophysics Data System (ADS)

Turnpenney, Sam; Nichols, Jonathan D.; Wynn, Graham A.; Burleigh, Matthew R.

2018-02-01

We consider the magnetic interaction of exoplanets orbiting M dwarfs, calculating the expected Poynting flux carried upstream along Alfvén wings to the central star. A region of emission analogous to the Io footprint observed in Jupiter’s aurora is produced, and we calculate the radio flux density generated near the surface of the star via the electron-cyclotron maser instability. We apply the model to produce individual case studies for the TRAPPIST-1, Proxima Centauri, and dwarf NGTS-1 systems. We predict steady-state flux densities of up to ∼10 μJy and sporadic bursts of emission of up to ∼1 mJy from each case study, suggesting these systems may be detectable with the Very Large Array and the Giant Metrewave Radio Telescope, and perhaps the Square Kilometre Array in the future. Finally, we present a survey of 85 exoplanets orbiting M dwarfs, identifying 11 such objects capable of generating radio emission above 10 μJy.

PROXIMA CENTAURI AS A BENCHMARK FOR STELLAR ACTIVITY INDICATORS IN THE NEAR-INFRARED

DOE Office of Scientific and Technical Information (OSTI.GOV)

Robertson, Paul; Bender, Chad; Mahadevan, Suvrath

A new generation of dedicated Doppler spectrographs will attempt to detect low-mass exoplanets around mid- to late M stars at near-infrared (NIR) wavelengths, where those stars are brightest and have the most Doppler information content. A central requirement for the success of these instruments is to properly measure the component of radial velocity (RV) variability contributed by stellar magnetic activity and to account for it in exoplanet models of RV data. The wavelength coverage for many of these new instruments will not include the Ca ii H and K or H α  lines, the most frequently used absorption-line tracers of magneticmore » activity. Thus, it is necessary to define and characterize NIR activity indicators for mid- to late M stars in order to provide simultaneous activity metrics for NIR RV data. We have used the high-cadence UVES observations of the M5.5 dwarf Proxima Centauri from Fuhrmeister et al. to compare the activity sensitivity of eight NIR atomic lines to that of H α . We find that equivalent-width-type measurements of the NIR K i doublet and the Ca ii NIR triplet are excellent proxies for the canonical optical tracers. The Ca ii triplet will be acquired by most of the new and upcoming NIR Doppler spectrographs, offering a common, reliable indicator of activity.« less

Rapidly rotating second-generation progenitors for the 'blue hook' stars of ω Centauri.

PubMed

Tailo, Marco; D'Antona, Francesca; Vesperini, Enrico; Di Criscienzo, Marcella; Ventura, Paolo; Milone, Antonino P; Bellini, Andrea; Dotter, Aaron; Decressin, Thibaut; D'Ercole, Annibale; Caloi, Vittoria; Capuzzo-Dolcetta, Roberto

2015-07-16

Horizontal branch stars belong to an advanced stage in the evolution of the oldest stellar galactic population, occurring either as field halo stars or grouped in globular clusters. The discovery of multiple populations in clusters that were previously believed to have single populations gave rise to the currently accepted theory that the hottest horizontal branch members (the 'blue hook' stars, which had late helium-core flash ignition, followed by deep mixing) are the progeny of a helium-rich 'second generation' of stars. It is not known why such a supposedly rare event (a late flash followed by mixing) is so common that the blue hook of ω Centauri contains approximately 30 per cent of the horizontal branch stars in the cluster, or why the blue hook luminosity range in this massive cluster cannot be reproduced by models. Here we report that the presence of helium core masses up to about 0.04 solar masses larger than the core mass resulting from evolution is required to solve the luminosity range problem. We model this by taking into account the dispersion in rotation rates achieved by the progenitors, whose pre-main-sequence accretion disk suffered an early disruption in the dense environment of the cluster's central regions, where second-generation stars form. Rotation may also account for frequent late-flash-mixing events in massive globular clusters.

The most detailed high-energy picture of Proxima Centauri, our nearest extrasolar neighbor

NASA Astrophysics Data System (ADS)

Schneider, Christian

2016-10-01

Proxima Centauri b is the nearest exoplanet to the Sun. It orbits an M5.5 dwarf and is potentially habitable. The latter statement, however, depends sensitively on the high-energy irradiation on the planet. Ribas et al. (2016) estimated the high-energy flux of the host star by collecting archival data from the X-ray to the FUV regime, but explicitly state that one unavoidable complication of estimating XUV fluxes is [...] intrinsic [stellar] variability. Here, we propose to greatly improve upon this unavoidable complication by obtaining simultaneous X-ray and UV observations to measure a high-resolution irradiation spectrum and, thus, to assess the habitability of Proxima b.Our upcoming, very deep Chandra grating observation of Proxima Cen (175 ks, LETGS, PI: P. Predehl) provides a great opportunity to obtain simultaneous coverage at X-ray and UV wavelengths, i.e., to measure most of the stellar high-energy flux in a coherent way. The reason for proposing a HST DDT is that the Chandra observation is a GTO and, thus, could not be augmented by simultaneous HST observations directly as we would have proposedfor in a regular GO.Combining Chandra X-ray and HST UV data allows us to reconstruct a high-resolution spectral energy distribution (SED) including the EUV regime and, thus, a reference irradiation spectrum using the methods developed by us for the MUSCLES project.

Assessments of Fuels for Military Use Preparation and Distribution of Synthetic Fuel Blends

DTIC Science & Technology

2013-01-01

received from DLA Energy. The HRJ-8 was sourced from Centuri Technologies LP. Three separate shipments of HRJ-8 fuel (18,450 gallons) were received...of HRJ-8 was received from Centauri Technologies , LP. Three separate shipments totaling 16,382 gallons were delivered to TFLRF on January 25, 26, and...for Patsy Muzzell U.S. Army TARDEC Force Projection Technologies Warren, Michigan Contract No. W56HZV-09-C-0100 (WD0004–Tasks 1, 20, 21

21 CFR 866.5420 - Alpha-1-glycoproteins immunological test system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Alpha-1-glycoproteins immunological test system....5420 Alpha-1-glycoproteins immunological test system. (a) Identification. An alpha-1-glycoproteins... alpha-1-glycoproteins (a group of plasma proteins found in the alpha-1 group when subjected to...

21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Alpha-1-antichymotrypsin immunological test system....5080 Alpha-1-antichymotrypsin immunological test system. (a) Identification. An alpha-1... immunochemical techniques alpha-1-antichymotrypsin (a protein) in serum, other body fluids, and tissues. Alpha-1...

21 CFR 866.5580 - Alpha-1-lipoprotein immuno-logical test system.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Alpha-1-lipoprotein immuno-logical test system....5580 Alpha-1-lipoprotein immuno-logical test system. (a) Identification. An alpha-1-lipoprotein... the alpha-1-lipoprotein (high-density lipoprotein) in serum and plasma. Measurement of alpha-1...

21 CFR 866.5620 - Alpha-2-macroglobulin immunological test system.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Alpha-2-macroglobulin immunological test system....5620 Alpha-2-macroglobulin immunological test system. (a) Identification. An alpha-2-macroglobulin... the alpha-2-macroglobulin (a serum protein) in plasma. Measurement of alpha-2-macroglobulin may aid in...

21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Alpha-1-antichymotrypsin immunological test system....5080 Alpha-1-antichymotrypsin immunological test system. (a) Identification. An alpha-1... immunochemical techniques alpha-1-antichymotrypsin (a protein) in serum, other body fluids, and tissues. Alpha-1...

21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Alpha-1-antichymotrypsin immunological test system....5080 Alpha-1-antichymotrypsin immunological test system. (a) Identification. An alpha-1... immunochemical techniques alpha-1-antichymotrypsin (a protein) in serum, other body fluids, and tissues. Alpha-1...

21 CFR 866.5620 - Alpha-2-macroglobulin immunological test system.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Alpha-2-macroglobulin immunological test system....5620 Alpha-2-macroglobulin immunological test system. (a) Identification. An alpha-2-macroglobulin... the alpha-2-macroglobulin (a serum protein) in plasma. Measurement of alpha-2-macroglobulin may aid in...

21 CFR 866.5620 - Alpha-2-macroglobulin immunological test system.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Alpha-2-macroglobulin immunological test system....5620 Alpha-2-macroglobulin immunological test system. (a) Identification. An alpha-2-macroglobulin... the alpha-2-macroglobulin (a serum protein) in plasma. Measurement of alpha-2-macroglobulin may aid in...

21 CFR 866.5580 - Alpha-1-lipoprotein immuno-logical test system.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Alpha-1-lipoprotein immuno-logical test system....5580 Alpha-1-lipoprotein immuno-logical test system. (a) Identification. An alpha-1-lipoprotein... the alpha-1-lipoprotein (high-density lipoprotein) in serum and plasma. Measurement of alpha-1...

21 CFR 866.5580 - Alpha-1-lipoprotein immuno-logical test system.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Alpha-1-lipoprotein immuno-logical test system....5580 Alpha-1-lipoprotein immuno-logical test system. (a) Identification. An alpha-1-lipoprotein... the alpha-1-lipoprotein (high-density lipoprotein) in serum and plasma. Measurement of alpha-1...

21 CFR 866.5580 - Alpha-1-lipoprotein immuno-logical test system.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Alpha-1-lipoprotein immuno-logical test system....5580 Alpha-1-lipoprotein immuno-logical test system. (a) Identification. An alpha-1-lipoprotein... the alpha-1-lipoprotein (high-density lipoprotein) in serum and plasma. Measurement of alpha-1...

21 CFR 866.5620 - Alpha-2-macroglobulin immunological test system.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Alpha-2-macroglobulin immunological test system....5620 Alpha-2-macroglobulin immunological test system. (a) Identification. An alpha-2-macroglobulin... the alpha-2-macroglobulin (a serum protein) in plasma. Measurement of alpha-2-macroglobulin may aid in...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kipping, David M.; Chen, Jingjing; Sandford, Emily

The analysis of Proxima Centauri’s radial velocities recently led Anglada-Escudé et al. to claim the presence of a low-mass planet orbiting the Sun’s nearest star once every 11.2 days. Although the a priori probability that Proxima b transits its parent star is just 1.5%, the potential impact of such a discovery would be considerable. Independent of recent radial velocity efforts, we observed Proxima Centauri for 12.5 days in 2014 and 31 days in 2015 with the Microwave and Oscillations of Stars space telescope. We report here that we cannot make a compelling case that Proxima b transits in our precisemore » photometric time series. Imposing an informative prior on the period and phase, we do detect a candidate signal with the expected depth. However, perturbing the phase prior across 100 evenly spaced intervals reveals one strong false positive and one weaker instance. We estimate a false-positive rate of at least a few percent and a much higher false-negative rate of 20%–40%, likely caused by the very high flare rate of Proxima Centauri. Comparing our candidate signal to HATSouth ground-based photometry reveals that the signal is somewhat, but not conclusively, disfavored (1 σ –2 σ ), leading us to argue that the signal is most likely spurious. We expect that infrared photometric follow-up could more conclusively test the existence of this candidate signal, owing to the suppression of flare activity and the impressive infrared brightness of the parent star.« less

No Conclusive Evidence for Transits of Proxima b in MOST Photometry

NASA Astrophysics Data System (ADS)

Kipping, David M.; Cameron, Chris; Hartman, Joel D.; Davenport, James R. A.; Matthews, Jaymie M.; Sasselov, Dimitar; Rowe, Jason; Siverd, Robert J.; Chen, Jingjing; Sandford, Emily; Bakos, Gáspár Á.; Jordán, Andrés; Bayliss, Daniel; Henning, Thomas; Mancini, Luigi; Penev, Kaloyan; Csubry, Zoltan; Bhatti, Waqas; Da Silva Bento, Joao; Guenther, David B.; Kuschnig, Rainer; Moffat, Anthony F. J.; Rucinski, Slavek M.; Weiss, Werner W.

2017-03-01

The analysis of Proxima Centauri’s radial velocities recently led Anglada-Escudé et al. to claim the presence of a low-mass planet orbiting the Sun’s nearest star once every 11.2 days. Although the a priori probability that Proxima b transits its parent star is just 1.5%, the potential impact of such a discovery would be considerable. Independent of recent radial velocity efforts, we observed Proxima Centauri for 12.5 days in 2014 and 31 days in 2015 with the Microwave and Oscillations of Stars space telescope. We report here that we cannot make a compelling case that Proxima b transits in our precise photometric time series. Imposing an informative prior on the period and phase, we do detect a candidate signal with the expected depth. However, perturbing the phase prior across 100 evenly spaced intervals reveals one strong false positive and one weaker instance. We estimate a false-positive rate of at least a few percent and a much higher false-negative rate of 20%-40%, likely caused by the very high flare rate of Proxima Centauri. Comparing our candidate signal to HATSouth ground-based photometry reveals that the signal is somewhat, but not conclusively, disfavored (1σ-2σ), leading us to argue that the signal is most likely spurious. We expect that infrared photometric follow-up could more conclusively test the existence of this candidate signal, owing to the suppression of flare activity and the impressive infrared brightness of the parent star.

On the Magnetic Protection of the Atmosphere of Proxima Centauri b

NASA Astrophysics Data System (ADS)

Garcia-Sage, K.; Glocer, A.; Drake, J. J.; Gronoff, G.; Cohen, O.

2017-07-01

The discovery of exoplanets orbiting red dwarfs, such as Proxima Centauri b, has led to questions of their habitability and capacity to retain liquid surface water. While Proxima b is in a “temperate orbit,” I.e., an Earth at that location would not freeze or boil its oceans, its proximity to a parent star with quite high magnetic activity is likely to influence its atmospheric evolution and habitability. Planetary magnetic fields can prevent direct stripping away of the planetary atmosphere by the stellar wind, but ion escape can still occur at the magnetic poles. This process, the polar wind, is well known to occur at Earth and may have contributed to the habitability of Earth’s early atmosphere. The polar wind is highly variable and sensitive to both ionizing radiation and geomagnetic activity. The higher ionizing radiation levels of M dwarfs at habitable zone distances are expected to increase the polar wind by orders of magnitude and, instead of helping create a habitable atmosphere, may strip away enough volatiles to render the planet inhospitable. Here, we compute the ionospheric outflow of an Earth-twin subject to the enhanced stellar EUV flux of Proxima b, and the effect on atmospheric escape timescales. We show that an Earth-like planet would not survive the escape of its atmosphere at that location, and therefore the pathway to habitability for Proxima b requires a very different atmospheric history than that of Earth.

Medium-resolution Spectroscopy of Red Giant Branch Stars in ω Centauri

NASA Astrophysics Data System (ADS)

An, Deokkeun; Lee, Young Sun; In Jung, Jae; Rey, Soo-Chang; Rhee, Jaehyon; Lee, Jae-Woo; Lee, Young-Wook; Joe, Young Hoon

2017-10-01

We present [Fe/H] and [Ca/Fe] of ˜600 red giant branch (RGB) members of the globular cluster Omega Centauri (ω {Cen}). We collect medium-resolution (R˜ 2000) spectra using the Blanco 4 m telescope at the Cerro Tololo Inter-American Observatory equipped with Hydra, the fiber-fed multi-object spectrograph. We demonstrate that blending of stellar light in optical fibers severely limits the accuracy of spectroscopic parameters in the crowded central region of the cluster. When photometric temperatures are taken in the spectroscopic analysis, our kinematically selected cluster members, excluding those that are strongly affected by flux from neighboring stars, include relatively fewer stars at intermediate metallicity ([{Fe}/{{H}}]˜ -1.5) than seen in the previous high-resolution survey for brighter giants in Johnson & Pilachowski. As opposed to the trend of increasing [Ca/Fe] with [Fe/H] found by those authors, our [Ca/Fe] estimates, based on Ca II H & K measurements, show essentially the same mean [Ca/Fe] for most of the metal-poor and metal-intermediate populations in this cluster, suggesting that mass- or metallicity-dependent SN II yields may not be necessary in their proposed chemical evolution scenario. Metal-rich cluster members in our sample show a large spread in [Ca/Fe], and do not exhibit a clear bimodal distribution in [Ca/Fe]. We also do not find convincing evidence for a radial metallicity gradient among RGB stars in ω {Cen}.

On the Magnetic Protection of the Atmosphere of Proxima Centauri b

DOE Office of Scientific and Technical Information (OSTI.GOV)

Garcia-Sage, K.; Glocer, A.; Drake, J. J.

The discovery of exoplanets orbiting red dwarfs, such as Proxima Centauri b, has led to questions of their habitability and capacity to retain liquid surface water. While Proxima b is in a “temperate orbit,” i.e., an Earth at that location would not freeze or boil its oceans, its proximity to a parent star with quite high magnetic activity is likely to influence its atmospheric evolution and habitability. Planetary magnetic fields can prevent direct stripping away of the planetary atmosphere by the stellar wind, but ion escape can still occur at the magnetic poles. This process, the polar wind, is wellmore » known to occur at Earth and may have contributed to the habitability of Earth’s early atmosphere. The polar wind is highly variable and sensitive to both ionizing radiation and geomagnetic activity. The higher ionizing radiation levels of M dwarfs at habitable zone distances are expected to increase the polar wind by orders of magnitude and, instead of helping create a habitable atmosphere, may strip away enough volatiles to render the planet inhospitable. Here, we compute the ionospheric outflow of an Earth-twin subject to the enhanced stellar EUV flux of Proxima b, and the effect on atmospheric escape timescales. We show that an Earth-like planet would not survive the escape of its atmosphere at that location, and therefore the pathway to habitability for Proxima b requires a very different atmospheric history than that of Earth.« less

Direct imaging of exoplanets in the habitable zone with adaptive optics

NASA Astrophysics Data System (ADS)

Males, Jared R.; Close, Laird M.; Guyon, Olivier; Morzinski, Katie; Puglisi, Alfio; Hinz, Philip; Follette, Katherine B.; Monnier, John D.; Tolls, Volker; Rodigas, Timothy J.; Weinberger, Alycia; Boss, Alan; Kopon, Derek; Wu, Ya-lin; Esposito, Simone; Riccardi, Armando; Xompero, Marco; Briguglio, Runa; Pinna, Enrico

2014-07-01

One of the primary goals of exoplanet science is to find and characterize habitable planets, and direct imaging will play a key role in this effort. Though imaging a true Earth analog is likely out of reach from the ground, the coming generation of giant telescopes will find and characterize many planets in and near the habitable zones (HZs) of nearby stars. Radial velocity and transit searches indicate that such planets are common, but imaging them will require achieving extreme contrasts at very small angular separations, posing many challenges for adaptive optics (AO) system design. Giant planets in the HZ may even be within reach with the latest generation of high-contrast imagers for a handful of very nearby stars. Here we will review the definition of the HZ, and the characteristics of detectable planets there. We then review some of the ways that direct imaging in the HZ will be different from the typical exoplanet imaging survey today. Finally, we present preliminary results from our observations of the HZ of α Centauri A with the Magellan AO system's VisAO and Clio2 cameras.

21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test Systems § 866.5080 Alpha-1-antichymotrypsin immunological test system. (a) Identification. An alpha-1... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Alpha-1-antichymotrypsin immunological test system...

The alpha Centauri Line of Sight: D/H Ratio, Physical Properties of Local Interstellar Gas, and Measurement of Heated Hydrogen (The 'Hydrogen Wall') Near the Heliopause

NASA Astrophysics Data System (ADS)

Linsky, Jeffrey L.; Wood, Brian E.

1996-05-01

We analyze high-resolution spectra of the nearby (1.34 pc) stars alpha Cen A (G2 V) and alpha Cen B (K1 V), which were obtained with the Goddard High Resolution Spectrograph on the Hubble Space Telescope. The observations consist of echelle spectra of the Mg II 2800 A and Fe II 2599 A resonance lines and the Lyman-alpha lines of hydrogen and deuterium. The interstellar gas has a velocity (v = - 18.0 +/- 0.2 km/s) consistent with the local flow vector proposed for this line of sight by Lailement & Berlin (1992). The temperature and nonthermal velocity inferred from the Fe II, Mg II, and D I line profiles are T = 5400 +/- 500 K and xi = 1.20 +/- 0.25 km/s, respectively. However, single-component fits to the H I Lyman-alpha lines yield a Doppler parameter (bHI = 11.80 km/s) that implies a significantly warmer temperature of 8350 K, and the velocity of the H I absorption (v = - 15.8 +/- 0.2 km/s) is redshifted by about 2.2 km/s with respect to the Fe II, Mg II, and D I lines. The one-component model of the interstellar gas suggests natural logarithm N base HI = 18.03 +/- 0.01 and D/H = (5.7 +/- 0.2) x 10-6 . These parameters lead to a good fit to the observed spectra, but this model does not explain the higher temperature and redshift of H I relative to the other interstellar lines. The most sensible way to resolve the discrepancy between H(I) and the other lines is to add a second absorption component to the H(I) lines. This second component is hotter (T approx. equals 30,000 K), is redshifted relative to the primary component by 2-4 km/s, and has a column density too low to be detected in the Fe(II), Mg(II), and D(I) lines. We propose that the gas responsible for this component is located near the heliopause, consisting of the heated H I gas from the interstellar medium that is compressed by the solar wind. This so-called 'hydrogen wall' is predicted by recent multifluid gasdynamical models of the interstellar gas and solar wind interaction. Our data provide the first measurements of the temperature and column density of H(I) in the hydrogen wall. After considering the effects that a corresponding hydrogen wall around alpha Cen would have on our analysis, our best estimates for the parameters of the solar hydrogen wall are natural log N(2)(H(I)) = 14.74 +/- 0.24, b(2)(H(I)) = 21.9 +/- 1.7 km/s (corresponding to T = 29,000 +/- 5000 K), and v(2)(H(I)) greater than -16km/s. Unfortunately, the existence of this heated H(I) reduces our ability to compute the H(I) column density of the interstellar medium accurately because, with slight alterations to our assumed stellar Lyman-alpha profiles, we discovered that acceptable two-component fits also exist with natural log N(H(I))approx. 17.6. We, therefore, quote large error bars for the H I column density along the alpha Cen line of sight, natural log N(H(I)) = 17.80 +/- 0.30. For this range in N(H(I)), n(H(I)) = 0.15 /cu.cm (+/- a factor of 2) and D/H = (0.5-1.9) x 10-5. This is the first direct measurement of the H(I) density in a local cloud and allows us to predict the distance from the Sun to the edge of the local cloud along various lines of sight. This range in D/H is consistent with the value D/H = 1.6 x 10-5 previously derived for the Capella and Procyon lines of sight. We cannot tell whether D/H ratio varies or is constant in the local interstellar medium, but we do find that the D(I)/Mg(II) ratio for the alpha Cen line of sight is about 4 times smaller than for the Capella and Procyon lines of sight. Therefore, either D/H or the Mg depletion varies significantly over distance scales of only a few parsecs.

21 CFR 866.5620 - Alpha-2-macroglobulin immunological test system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test Systems § 866.5620 Alpha-2-macroglobulin immunological test system. (a) Identification. An alpha-2-macroglobulin... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Alpha-2-macroglobulin immunological test system...

21 CFR 866.5130 - Alpha-1-antitrypsin immunological test system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test Systems § 866.5130 Alpha-1-antitrypsin immunological test system. (a) Identification. An alpha-1-antitrypsin... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Alpha-1-antitrypsin immunological test system. 866...

21 CFR 866.5400 - Alpha-globulin immuno-logical test system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test Systems § 866.5400 Alpha-globulin immuno-logical test system. (a) Identification. An alpha-globulin immunological... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Alpha-globulin immuno-logical test system. 866...

21 CFR 866.5580 - Alpha-1-lipoprotein immuno-logical test system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test Systems § 866.5580 Alpha-1-lipoprotein immuno-logical test system. (a) Identification. An alpha-1-lipoprotein... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Alpha-1-lipoprotein immuno-logical test system...

The metallicity spread and the age-metallicity relation of ω Centauri

DOE Office of Scientific and Technical Information (OSTI.GOV)

Villanova, S.; Geisler, D.; Gratton, R. G.

2014-08-20

ω Centauri is a peculiar globular cluster formed by a complex stellar population. To investigate it, we studied 172 stars belonging to the five SGBs that we can identify in our photometry, in order to measure their [Fe/H] content as well as estimate their age dispersion and the age-metallicity relation. The first important result is that all of these SGBs have a distribution in metallicity with a spread that exceeds the observational errors and typically displays several peaks that indicate the presence of several subpopulations. We were able to identify at least six of them based on their mean [Fe/H]more » content. These metallicity-based subpopulations are seen to varying extents in each of the five SGBs. Taking advantage of the age sensitivity of the SGB, we showed that, first of all, at least half of the subpopulations have an age spread of at least 2 Gyr. Then, we obtained an age-metallicity relation that is the most complete to date for this cluster. Interpretation of the age-metallicity relation is not straightforward, but it is possible that the cluster (or what we can call its progenitor) was initially composed of two populations with different metallicities. Because of their age, it is very unlikely that the most metal-rich derives from the most metal-poor by some kind of chemical evolution process, so they can be assumed to be two independent primordial objects, or perhaps two separate parts of a single larger object, that merged in the past to form the present-day cluster.« less

Magnetic properties of Proxima Centauri b analogues

NASA Astrophysics Data System (ADS)

Zuluaga, Jorge I.; Bustamante, Sebastian

2018-03-01

The discovery of a planet around the closest star to our Sun, Proxima Centauri, represents a quantum leap in the testability of exoplanetary models. Unlike any other discovered exoplanet, models of Proxima b could be contrasted against near future telescopic observations and far future in-situ measurements. In this paper we aim at predicting the planetary radius and the magnetic properties (dynamo lifetime and magnetic dipole moment) of Proxima b analogues (solid planets with masses of ∼ 1 - 3M⊕ , rotation periods of several days and habitable conditions). For this purpose we build a grid of planetary models with a wide range of compositions and masses. For each point in the grid we run the planetary evolution model developed in Zuluaga et al. (2013). Our model assumes small orbital eccentricity, negligible tidal heating and earth-like radiogenic mantle elements abundances. We devise a statistical methodology to estimate the posterior distribution of the desired planetary properties assuming simple lprior distributions for the orbital inclination and bulk composition. Our model predicts that Proxima b would have a mass 1.3 ≤Mp ≤ 2.3M⊕ and a radius Rp =1.4-0.2+0.3R⊕ . In our simulations, most Proxima b analogues develop intrinsic dynamos that last for ≥4 Gyr (the estimated age of the host star). If alive, the dynamo of Proxima b have a dipole moment ℳdip >0.32÷2.9×2.3ℳdip , ⊕ . These results are not restricted to Proxima b but they also apply to earth-like planets having similar observed properties.

Increased virulence and competitive advantage of a/alpha over a/a or alpha/alpha offspring conserves the mating system of Candida albicans.

PubMed

Lockhart, Shawn R; Wu, Wei; Radke, Joshua B; Zhao, Rui; Soll, David R

2005-04-01

The majority of Candida albicans strains in nature are a/alpha and must undergo homozygosis to a/a or alpha/alpha to mate. Here we have used a mouse model for systemic infection to test the hypothesis that a/alpha strains predominate in nature because they have a competitive advantage over a/a and alpha/alpha offspring in colonizing hosts. Single-strain injection experiments revealed that a/alpha strains were far more virulent than either their a/a or alpha/alpha offspring. When equal numbers of parent a/alpha and offspring a/a or alpha/alpha cells were co-injected, a/alpha always exhibited a competitive advantage at the time of extreme host morbidity or death. When equal numbers of an engineered a/a/alpha2 strain and its isogenic a/a parent strain were co-injected, the a/a/alpha2 strain exhibited a competitive advantage at the time of host morbidity or death, suggesting that the genotype of the mating-type (MTL) locus, not associated genes on chromosome 5, provides a competitive advantage. We therefore propose that heterozygosity at the MTL locus not only represses white-opaque switching and genes involved in the mating process, but also affects virulence, providing a competitive advantage to the a/alpha genotype that conserves the mating system of C. albicans in nature.

Regulation of tumour necrosis factor production by adrenal hormones in vivo: insights into the antiinflammatory activity of rolipram.

PubMed Central

Pettipher, E. R.; Labasi, J. M.; Salter, E. D.; Stam, E. J.; Cheng, J. B.; Griffiths, R. J.

1996-01-01

1. The role of adrenal hormones in the regulation of the systemic and local production of tumour necrosis factor (TNF alpha) was examined in male Balb/c mice. 2. Intraperitoneal injection of 0.3 mg E. coli lipopolysaccharide (LPS, 0111:B4) led to high levels of circulating TNF alpha without stimulating TNF alpha production in the peritoneal cavity. Systemic production of TNF alpha in response to LPS was increased in adrenalectomized animals and in normal animals treated with the beta-adrenoceptor antagonist, propranolol. The glucocorticoid antagonist, RU 486, did not modify systemic TNF alpha production. These results indicate that systemic TNF alpha production is regulated by adrenaline but not by corticosterone. 3. When mice were primed with thioglycollate, TNF alpha was produced in the peritoneal cavity in response to low dose LPS (1 micrograms). The levels of TNF alpha in the peritoneal cavity were not enhanced by adrenalectomy or by treatment with either propranolol or RU 486, indicating local production of TNF alpha in the peritoneal cavity is not regulated by adrenaline or corticosterone. 4. The phosphodiesterase type IV (PDE-IV) inhibitor, rolipram, inhibited both the systemic production of TNF alpha in response to high dose endotoxin (ED50 = 1.3 mg kg-1) and the local production of TNF alpha in the peritoneal cavity in response to low dose endotoxin (ED50 = 9.1 mg kg-1). In adrenalectomized mice there was a slight reduction in the ability of rolipram to inhibit the systemic production of TNF alpha (ED50 = 3.3 mg kg-1) while the ability of rolipram to inhibit the local production of TNF alpha in the peritoneal cavity was virtually abolished (24% inhibition at 30 mg kg-1). The glucocorticoid antagonist, RU 486, also reduced the ability of rolipram to inhibit local TNF alpha production while propranolol was without effect. 5. Systemic treatment with rolipram increased the plasma concentrations of corticosterone in normal mice but not in adrenalectomized mice indicating that rolipram can cause adrenal stimulation in vivo. 6. In summary, these data indicate that systemic production of TNF alpha in response to high dose endotoxin is controlled differently from the local production of TNF alpha in response to low dose endotoxin. The systemic production of TNF alpha is regulated by catecholamines, but not by corticosterone, while the local production of TNF alpha in the peritoneal cavity is not regulated by basal levels of either catecholamines or corticosterone. 7. These data also show that the ability of rolipram to inhibit the local production of TNF alpha is dependent on the release of corticosterone from the adrenal glands. PMID:8730750

Regulation of tumour necrosis factor production by adrenal hormones in vivo: insights into the antiinflammatory activity of rolipram.

PubMed

Pettipher, E R; Labasi, J M; Salter, E D; Stam, E J; Cheng, J B; Griffiths, R J

1996-04-01

1. The role of adrenal hormones in the regulation of the systemic and local production of tumour necrosis factor (TNF alpha) was examined in male Balb/c mice. 2. Intraperitoneal injection of 0.3 mg E. coli lipopolysaccharide (LPS, 0111:B4) led to high levels of circulating TNF alpha without stimulating TNF alpha production in the peritoneal cavity. Systemic production of TNF alpha in response to LPS was increased in adrenalectomized animals and in normal animals treated with the beta-adrenoceptor antagonist, propranolol. The glucocorticoid antagonist, RU 486, did not modify systemic TNF alpha production. These results indicate that systemic TNF alpha production is regulated by adrenaline but not by corticosterone. 3. When mice were primed with thioglycollate, TNF alpha was produced in the peritoneal cavity in response to low dose LPS (1 micrograms). The levels of TNF alpha in the peritoneal cavity were not enhanced by adrenalectomy or by treatment with either propranolol or RU 486, indicating local production of TNF alpha in the peritoneal cavity is not regulated by adrenaline or corticosterone. 4. The phosphodiesterase type IV (PDE-IV) inhibitor, rolipram, inhibited both the systemic production of TNF alpha in response to high dose endotoxin (ED50 = 1.3 mg kg-1) and the local production of TNF alpha in the peritoneal cavity in response to low dose endotoxin (ED50 = 9.1 mg kg-1). In adrenalectomized mice there was a slight reduction in the ability of rolipram to inhibit the systemic production of TNF alpha (ED50 = 3.3 mg kg-1) while the ability of rolipram to inhibit the local production of TNF alpha in the peritoneal cavity was virtually abolished (24% inhibition at 30 mg kg-1). The glucocorticoid antagonist, RU 486, also reduced the ability of rolipram to inhibit local TNF alpha production while propranolol was without effect. 5. Systemic treatment with rolipram increased the plasma concentrations of corticosterone in normal mice but not in adrenalectomized mice indicating that rolipram can cause adrenal stimulation in vivo. 6. In summary, these data indicate that systemic production of TNF alpha in response to high dose endotoxin is controlled differently from the local production of TNF alpha in response to low dose endotoxin. The systemic production of TNF alpha is regulated by catecholamines, but not by corticosterone, while the local production of TNF alpha in the peritoneal cavity is not regulated by basal levels of either catecholamines or corticosterone. 7. These data also show that the ability of rolipram to inhibit the local production of TNF alpha is dependent on the release of corticosterone from the adrenal glands.

Elemental abundance analyses with Complejo Astronomico EL Leoncito REOSC echelle spectrograms. III. HR 4487, 14 Hydrae, and 3 Centauri A

NASA Astrophysics Data System (ADS)

Pintado, O. I.; Adelman, S. J.; Gulliver, A. F.

1998-05-01

Using CASLEO echelle spectrograms, elemental abundances are derived for the sharp-lined non-magnetic CP stars HR 4487, 14 Hya, and 3 Cen A. The first two stars are members of the Mercury-Manganese subgroup and have abundances which are similar to other such peculiar stars. The third is a hotter related star. The detection of Mn II lines in its spectrum adds to this relationship. Table 3 is avaible electronically vit the CDS via anonymous ftp 130.79.128.5 or http://cdsweb.u-strasbg.fr/Abstract.html.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suhara, Tadahiro; Kanada-En'yo, Yoshiko

We investigate the linear-chain structures in highly excited states of {sup 14}C using a generalized molecular-orbital model, by which we incorporate an asymmetric configuration of three {alpha} clusters in the linear-chain states. By applying this model to the {sup 14}C system, we study the {sup 10}Be+{alpha} correlation in the linear-chain state of {sup 14}C. To clarify the origin of the {sup 10}Be+{alpha} correlation in the {sup 14}C linear-chain state, we analyze linear 3 {alpha} and 3{alpha} + n systems in a similar way. We find that a linear 3{alpha} system prefers the asymmetric 2{alpha} + {alpha} configuration, whose origin ismore » the many-body correlation incorporated by the parity projection. This configuration causes an asymmetric mean field for two valence neutrons, which induces the concentration of valence neutron wave functions around the correlating 2{alpha}. A linear-chain structure of {sup 16}C is also discussed.« less

Stellar Echo Imaging of Exoplanets

NASA Technical Reports Server (NTRS)

Mann, Chris; Lerch, Kieran; Lucente, Mark; Meza-Galvan, Jesus; Mitchell, Dan; Ruedin, Josh; Williams, Spencer; Zollars, Byron

2016-01-01

All stars exhibit intensity fluctuations over several timescales, from nanoseconds to years. These intensity fluctuations echo off bodies and structures in the star system. We posit that it is possible to take advantage of these echoes to detect, and possibly image, Earth-scale exoplanets. Unlike direct imaging techniques, temporal measurements do not require fringe tracking, maintaining an optically-perfect baseline, or utilizing ultra-contrast coronagraphs. Unlike transit or radial velocity techniques, stellar echo detection is not constrained to any specific orbital inclination. Current results suggest that existing and emerging technology can already enable stellar echo techniques at flare stars, such as Proxima Centauri, including detection, spectroscopic interrogation, and possibly even continent-level imaging of exoplanets in a variety of orbits. Detection of Earth-like planets around Sun-like stars appears to be extremely challenging, but cannot be fully quantified without additional data on micro- and millisecond-scale intensity fluctuations of the Sun. We consider survey missions in the mold of Kepler and place preliminary constraints on the feasibility of producing 3D tomographic maps of other structures in star systems, such as accretion disks. In this report we discuss the theory, limitations, models, and future opportunities for stellar echo imaging.

Modeled 3-D Biosignatures from the Stratospheres of Proxima Centauri b and M-dwarf Planets

NASA Astrophysics Data System (ADS)

Chen, Howard; Horton, Daniel

2018-01-01

Proxima Centauri b is one of the most promising extrasolar terrestrial planets to search for potential biomarkers due to its proximity to Earth and relatively high planet to stellar luminosity ratio. These factors create a prime target for follow-up characterization efforts by e.g., James Webb Space Telescope and/or directing imaging. High-resolution, 3-D model predictions of atmospheric biosignatures however, are not currently available in the community. Here we use the CESM1 WACCM, a high-top coupled climate-chemistry general circulation model, to simulate the circulation, photochemistry, and stratospheric chemistry of Proxima b. From our equilibrium simulations with boundary conditions consistent with Proxima b observations (i.e., mass, radius, heliocentric distance, etc.) and a stellar spectrum consistent with its host star, we find increased mixing ratios and lifetimes for biogenic compounds (e.g., CH4, N2O, and CH3Cl) in the stratosphere. Whereas these biogenic gases are typically concentrated at the equator on Earth, they are dispersed across the mid-latitudes and even to the poles of Proxima b. Our initial analysis suggests that these characteristics are the result of a markedly energized stratospheric circulation regime and altered photochemistry, both of which are the consequence of enhanced UV and IR radiative forcing relative to Earth. Model simulated global distribution and longer lifetimes of biomarkers suggest that Proxima b’s molecular absorption and observational windows are potentially greater than anticipated. These results indicate that the prospects for detecting signals of life on Proxima b and/or other M-dwarf planets are enhanced – a conclusion consistent with several prior studies using 1-D models.

Near-IR period-luminosity relations for pulsating stars in ω Centauri (NGC 5139)

NASA Astrophysics Data System (ADS)

Navarrete, C.; Catelan, M.; Contreras Ramos, R.; Alonso-García, J.; Gran, F.; Dékány, I.; Minniti, D.

2017-08-01

Aims: The globular cluster ω Centauri (NGC 5139) hosts hundreds of pulsating variable stars of different types, thus representing a treasure trove for studies of their corresponding period-luminosity (PL) relations. Our goal in this study is to obtain the PL relations for RR Lyrae and SX Phoenicis stars in the field of the cluster, based on high-quality, well-sampled light curves in the near-infrared (IR). Methods: Observations were carried out using the VISTA InfraRed CAMera (VIRCAM) mounted on the Visible and Infrared Survey Telescope for Astronomy (VISTA). A total of 42 epochs in J and 100 epochs in KS were obtained, spanning 352 days. Point-spread function photometry was performed using DoPhot and DAOPHOT crowded-field photometry packages in the outer and inner regions of the cluster, respectively. Results: Based on the comprehensive catalog of near-IR light curves thus secured, PL relations were obtained for the different types of pulsators in the cluster, both in the J and KS bands. This includes the first PL relations in the near-IR for fundamental-mode SX Phoenicis stars. The near-IR magnitudes and periods of Type II Cepheids and RR Lyrae stars were used to derive an updated true distance modulus to the cluster, with a resulting value of (m - M)0 = 13.708 ± 0.035 ± 0.10 mag, where the error bars correspond to the adopted statistical and systematic errors, respectively. Adding the errors in quadrature, this is equivalent to a heliocentric distance of 5.52 ± 0.27 kpc. Based on observations collected at the European Organisation for Astronomical Research in the Southern Hemisphere, Chile, with the VISTA telescope (project ID 087.D-0472, PI R. Angeloni).

Relativistic generalization of the incentive trap of interstellar travel with application to Breakthrough Starshot

NASA Astrophysics Data System (ADS)

Heller, René

2017-09-01

As new concepts of sending interstellar spacecraft to the nearest stars are now being investigated by various research teams, crucial questions about the timing of such a vast financial and labour investment arise. If humanity could build high-speed interstellar lightsails and reach α Centauri 20 yr after launch, would it be better to wait a few years, then take advantage of further technology improvements and arrive earlier despite waiting? The risk of being overtaken by a future, faster probe has been described earlier as the incentive trap. Based on 211 yr of historical data, we find that the speed growth of artificial vehicles, from steam-driven locomotives to Voyager 1, is much faster than previously believed, about 4.72 per cent annually or a doubling every 15 yr. We derive the mathematical framework to calculate the minimum of the wait time to launch t plus travel time τ(t) and extend it into the relativistic regime. We show that the t + τ(t) minimum disappears for nearby targets. There is no use of waiting once we can reach an object within about 20 yr of travel, irrespective of the actual speed. In terms of speed, the t + τ(t) minimum for a travel to α Centauri occurs at 19.6 per cent the speed of light (c), in agreement with the 20 per cent c proposed by the Breakthrough Starshot initiative. If interstellar travel at 20 per cent c could be achieved within 45 yr from today and the kinetic energy be increased at a rate consistent with the historical record, then humans can reach the 10 most nearby stars within 100 yr from today.

Optical, UV, and X-ray evidence for a 7-yr stellar cycle in Proxima Centauri

NASA Astrophysics Data System (ADS)

Wargelin, B. J.; Saar, S. H.; Pojmański, G.; Drake, J. J.; Kashyap, V. L.

2017-01-01

Stars of stellar type later than about M3.5 are believed to be fully convective and therefore unable to support magnetic dynamos like the one that produces the 11-yr solar cycle. Because of their intrinsic faintness, very few late M stars have undergone long-term monitoring to test this prediction, which is critical to our understanding of magnetic field generation in such stars. Magnetic activity is also of interest as the driver of UV and X-ray radiation, as well as energetic particles and stellar winds, that affects the atmospheres of close-in planets that lie within habitable zones, such as the recently discovered Proxima b. We report here on several years of optical, UV, and X-ray observations of Proxima Centauri (GJ 551; dM5.5e): 15 yr of All Sky Automated Survey photometry in the V band (1085 nights) and 3 yr in the I band (196 nights), 4 yr of Swift X-Ray Telescope and UV/Optical Telescope observations (more than 120 exposures), and nine sets of X-ray observations from other X-ray missions (ASCA, XMM-Newton, and three Chandra instruments) spanning 22 yr. We confirm previous reports of an 83-d rotational period and find strong evidence for a 7-yr stellar cycle, along with indications of differential rotation at about the solar level. X-ray/UV intensity is anticorrelated with optical V-band brightness for both rotational and cyclical variations. From comparison with other stars observed to have X-ray cycles, we deduce a simple empirical relationship between X-ray cyclic modulation and Rossby number, and we also present Swift UV grism spectra covering 2300-6000 Å.

Multiple alpha subunits of integrin are involved in cell-mediated responses of the Manduca immune system.

PubMed

Zhuang, Shufei; Kelo, Lisha; Nardi, James B; Kanost, Michael R

2008-01-01

The cell-mediated responses of the insect innate immune system-phagocytosis, nodulation, encapsulation-involve multiple cell adhesion molecules of hemocyte surfaces. A hemocyte-specific (HS) integrin and a member of the immunoglobulin (Ig) superfamily (neuroglian) are involved in the encapsulation response of hemocytes in Manduca sexta. In addition, two new integrin alpha (alpha) subunits have been found on these hemocytes. The alpha2 subunit is mainly expressed in epidermis and Malphigian tubules, whereas the alpha3 subunit is primarily expressed on hemocytes and fat body cells. Of the three known alpha subunits, the alpha1 subunit found in HS integrin is the predominant subunit of hemocytes. Cell adhesion assays indicate that alpha2 belongs to the integrin family with RGD-binding motifs, confirming the phylogenetic analysis of alpha subunits based on the amino-acid sequence alignment of different alpha subunits. Double-stranded RNAs (dsRNAs) targeting each of these three integrin alpha subunits not only specifically decreased transcript expression of each alpha subunit in hemocytes, but also abolished the cell-mediated encapsulation response of hemocytes to foreign surfaces. The individual alpha subunits of M. sexta integrins, like their integrin counterparts in mammalian immune systems, have critical, individual roles in cell-substrate and cell-cell interactions during immune responses.

21 CFR 866.5400 - Alpha-globulin immuno-logical test system.

Code of Federal Regulations, 2013 CFR

2013-04-01

... alpha-globulin (a serum protein) in serum and other body fluids. Measurement of alpha-globulin may aid... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Alpha-globulin immuno-logical test system. 866.5400 Section 866.5400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5400 - Alpha-globulin immuno-logical test system.

Code of Federal Regulations, 2012 CFR

2012-04-01

... alpha-globulin (a serum protein) in serum and other body fluids. Measurement of alpha-globulin may aid... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Alpha-globulin immuno-logical test system. 866.5400 Section 866.5400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5400 - Alpha-globulin immuno-logical test system.

Code of Federal Regulations, 2014 CFR

2014-04-01

... alpha-globulin (a serum protein) in serum and other body fluids. Measurement of alpha-globulin may aid... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Alpha-globulin immuno-logical test system. 866.5400 Section 866.5400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5400 - Alpha-globulin immuno-logical test system.

Code of Federal Regulations, 2011 CFR

2011-04-01

... alpha-globulin (a serum protein) in serum and other body fluids. Measurement of alpha-globulin may aid... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Alpha-globulin immuno-logical test system. 866.5400 Section 866.5400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

The alpha Centauri Line of Sight: D/H Ratio, Physical Properties of Local Interstellar Gas, and Measurement of Heated Hydrogen (The 'Hydrogen Wall') Near the Heliopause

NASA Technical Reports Server (NTRS)

Linsky, Jeffrey L.; Wood, Brian E.

1996-01-01

We analyze high-resolution spectra of the nearby (1.34 pc) stars alpha Cen A (G2 V) and alpha Cen B (K1 V), which were obtained with the Goddard High Resolution Spectrograph on the Hubble Space Telescope. The observations consist of echelle spectra of the Mg II 2800 A and Fe II 2599 A resonance lines and the Lyman-alpha lines of hydrogen and deuterium. The interstellar gas has a velocity (v = - 18.0 +/- 0.2 km/s) consistent with the local flow vector proposed for this line of sight by Lailement & Berlin (1992). The temperature and nonthermal velocity inferred from the Fe II, Mg II, and D I line profiles are T = 5400 +/- 500 K and xi = 1.20 +/- 0.25 km/s, respectively. However, single-component fits to the H I Lyman-alpha lines yield a Doppler parameter (b(sub HI) = 11.80 km/s) that implies a significantly warmer temperature of 8350 K, and the velocity of the H I absorption (v = - 15.8 +/- 0.2 km/s) is redshifted by about 2.2 km/s with respect to the Fe II, Mg II, and D I lines. The one-component model of the interstellar gas suggests natural logarithm N base HI = 18.03 +/- 0.01 and D/H = (5.7 +/- 0.2) x 10(exp -6) . These parameters lead to a good fit to the observed spectra, but this model does not explain the higher temperature and redshift of H I relative to the other interstellar lines. The most sensible way to resolve the discrepancy between H(I) and the other lines is to add a second absorption component to the H(I) lines. This second component is hotter (T approx. equals 30,000 K), is redshifted relative to the primary component by 2-4 km/s, and has a column density too low to be detected in the Fe(II), Mg(II), and D(I) lines. We propose that the gas responsible for this component is located near the heliopause, consisting of the heated H I gas from the interstellar medium that is compressed by the solar wind. This so-called 'hydrogen wall' is predicted by recent multifluid gasdynamical models of the interstellar gas and solar wind interaction. Our data provide the first measurements of the temperature and column density of H(I) in the hydrogen wall. After considering the effects that a corresponding hydrogen wall around alpha Cen would have on our analysis, our best estimates for the parameters of the solar hydrogen wall are natural log N(sup (2))(H(I)) = 14.74 +/- 0.24, b(sup (2))(H(I)) = 21.9 +/- 1.7 km/s (corresponding to T = 29,000 +/- 5000 K), and v(sup (2))(H(I)) greater than -16km/s. Unfortunately, the existence of this heated H(I) reduces our ability to compute the H(I) column density of the interstellar medium accurately because, with slight alterations to our assumed stellar Lyman-alpha profiles, we discovered that acceptable two-component fits also exist with natural log N(H(I))approx. 17.6. We, therefore, quote large error bars for the H I column density along the alpha Cen line of sight, natural log N(H(I)) = 17.80 +/- 0.30. For this range in N(H(I)), n(H(I)) = 0.15 /cu.cm (+/- a factor of 2) and D/H = (0.5-1.9) x 10(exp -5). This is the first direct measurement of the H(I) density in a local cloud and allows us to predict the distance from the Sun to the edge of the local cloud along various lines of sight. This range in D/H is consistent with the value D/H = 1.6 x 10(exp -5) previously derived for the Capella and Procyon lines of sight. We cannot tell whether D/H ratio varies or is constant in the local interstellar medium, but we do find that the D(I)/Mg(II) ratio for the alpha Cen line of sight is about 4 times smaller than for the Capella and Procyon lines of sight. Therefore, either D/H or the Mg depletion varies significantly over distance scales of only a few parsecs.

21 CFR 866.5890 - Inter-alpha trypsin inhibitor immunological test system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test Systems § 866.5890 Inter-alpha trypsin inhibitor immunological test system. (a) Identification. An inter... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Inter-alpha trypsin inhibitor immunological test...

Extrinsic factors regulate partial agonist efficacy of strychnine-sensitive glycine receptors

PubMed Central

Farroni, Jeffrey S; McCool, Brian A

2004-01-01

Background Strychnine-sensitive glycine receptors in many adult forebrain regions consist of alpha2 + beta heteromeric channels. This subunit composition is distinct from the alpha1 + beta channels found throughout the adult spinal cord. Unfortunately, the pharmacology of forebrain alpha2beta receptors are poorly defined compared to 'neonatal' alpha2 homomeric channels or 'spinal' alpha1beta heteromers. In addition, the pharmacologic properties of native alpha2beta glycine receptors have been generally distinct from receptors produced by heterologous expression. To identify subtype-specific pharmacologic tools for the forebrain alpha2beta receptors, it is important to identify a heterologous expression system that closely resembles these native glycine-gated chloride channels. Results While exploring pharmacological properties of alpha2beta glycine receptors compared to alpha2-homomers, we found that distinct heterologous expression systems appeared to differentially influence partial agonist pharmacology. The β-amino acid taurine possessed 30–50% efficacy for alpha2-containing receptor isoforms when expressed in HEK 293 cells. However, taurine efficacy was dramatically reduced in L-cell fibroblasts. Similar results were obtained for β-alanine. The efficacy of these partial agonists was also strongly reduced by the beta subunit. There were no significant differences in apparent strychnine affinity values calculated from concentration-response data between expression systems or subunit combinations. Nor did relative levels of expression correlate with partial agonist efficacy when compared within or between several different expression systems. Finally, disruption of the tubulin cytoskeleton reduced the efficacy of partial agonists in a subunit-dependent, but system-independent, fashion. Conclusions Our results suggest that different heterologous expression systems can dramatically influence the agonist pharmacology of strychnine-sensitive glycine receptors. In the systems examine here, these effects are independent of both absolute expression level and any system-related alterations in the agonist binding site. We conclude that complex interactions between receptor composition and extrinsic factors may play a significant role in determining strychnine-sensitive glycine receptor partial agonist pharmacology. PMID:15301692

Extrinsic factors regulate partial agonist efficacy of strychnine-sensitive glycine receptors.

PubMed

Farroni, Jeffrey S; McCool, Brian A

2004-08-09

Strychnine-sensitive glycine receptors in many adult forebrain regions consist of alpha2 + beta heteromeric channels. This subunit composition is distinct from the alpha1 + beta channels found throughout the adult spinal cord. Unfortunately, the pharmacology of forebrain alpha2beta receptors are poorly defined compared to 'neonatal' alpha2 homomeric channels or 'spinal' alpha1beta heteromers. In addition, the pharmacologic properties of native alpha2beta glycine receptors have been generally distinct from receptors produced by heterologous expression. To identify subtype-specific pharmacologic tools for the forebrain alpha2beta receptors, it is important to identify a heterologous expression system that closely resembles these native glycine-gated chloride channels. While exploring pharmacological properties of alpha2beta glycine receptors compared to alpha2-homomers, we found that distinct heterologous expression systems appeared to differentially influence partial agonist pharmacology. The beta-amino acid taurine possessed 30-50% efficacy for alpha2-containing receptor isoforms when expressed in HEK 293 cells. However, taurine efficacy was dramatically reduced in L-cell fibroblasts. Similar results were obtained for beta-alanine. The efficacy of these partial agonists was also strongly reduced by the beta subunit. There were no significant differences in apparent strychnine affinity values calculated from concentration-response data between expression systems or subunit combinations. Nor did relative levels of expression correlate with partial agonist efficacy when compared within or between several different expression systems. Finally, disruption of the tubulin cytoskeleton reduced the efficacy of partial agonists in a subunit-dependent, but system-independent, fashion. Our results suggest that different heterologous expression systems can dramatically influence the agonist pharmacology of strychnine-sensitive glycine receptors. In the systems examine here, these effects are independent of both absolute expression level and any system-related alterations in the agonist binding site. We conclude that complex interactions between receptor composition and extrinsic factors may play a significant role in determining strychnine-sensitive glycine receptor partial agonist pharmacology.

Five-Body Cluster Structure of the Double-{Lambda} Hypernucleus {sub {Lambda}{Lambda}}{sup 11}Be

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hiyama, E.; Kamimura, M.; Yamamoto, Y.

2010-05-28

Energy levels of the double {Lambda} hypernucleus, {sub {Lambda}{Lambda}}{sup 11}Be are calculated within the framework of a {alpha}{alpha}n{Lambda}{Lambda} five-body model. Interactions between constituent particles are determined so as to reproduce reasonably the observed low-energy properties of the {alpha}{alpha}, {alpha}{alpha}n nuclei and the existing data for {Lambda}-binding energies of the {alpha}{Lambda}, {alpha}{alpha}{Lambda}, {alpha}n{Lambda}, and {alpha}{alpha}n{Lambda} systems. An effective {Lambda}{Lambda} interaction is constructed so as to reproduce, within the {alpha}{Lambda}{Lambda} three-body model, the B{sub {Lambda}{Lambda}}of {sub {Lambda}{Lambda}}{sup 6}He, which was extracted from the emulsion experiment, the NAGARA event. With no adjustable parameters for the {alpha}{alpha}n{Lambda}{Lambda} system, B{sub {Lambda}{Lambda}}of the ground and boundmore » excited states of {sub {Lambda}{Lambda}}{sup 11}Be are calculated with the Gaussian expansion method. The Hida event, recently observed at KEK-E373 experiment, is interpreted as an observation of the ground state of the {sub {Lambda}{Lambda}}{sup 11}Be.« less

Nicotinic acetylcholine receptor alpha5 subunits modulate oxotremorine-induced salivation and tremor.

PubMed

Wang, Ningshan; Orr-Urtreger, Avi; Chapman, Joab; Rabinowitz, Ruth; Korczyn, Amos D

2004-07-15

Neuronal nicotinic acetylcholine receptors (nAChRs) are composed of 12 subunits (alpha2-alpha10 and beta2-beta4). alpha5 Subunits, expressed throughout the central nervous system (CNS) and the autonomic nervous system (ANS), possess unique pharmacological properties. The effects of oxotremorine (OXO) on autonomic functions and tremor were examined in mice lacking alpha5 nAChR subunits (alpha5-/-) and compared with those in wild-type (WT) control mice. The alpha5-/- mice showed significantly increased salivation and tremor responses to OXO. The hypothermia, bradycardia and defecation induced by OXO were of similar magnitudes in the two mouse strains. The enhanced OXO effects in alpha5-/- mice indicate inhibitory effects of alpha5 subunits in autonomic ganglia, and support the participation of these subunits in cholinergic transmission in autonomic ganglia.

Spatial resolution properties of digital autoradiography systems for pre-clinical alpha particle imaging (Conference Presentation)

NASA Astrophysics Data System (ADS)

Tanguay, Jesse; Benard, Francois; Celler, Anna; Ruth, Thomas; Schaffer, Paul

2017-03-01

Attaching alpha-emitting radionuclides to cancer-targeting agents increases the anti-tumor effects of targeted cancer therapies. The success of alpha therapy for treating bone metastases has increased interest in using targeted alpha therapy (TAT) to treat a broad spectrum of metastatic cancers. Estimating radiation doses to targeted tumors, including small (<250 μm) clusters of cancer cells, and to non-targeted tissues is critical in the pre-clinical development of TATs. However, accurate quantification of heterogeneous distributions of alpha-emitters in small metastases is not possible with existing pre-clinical in-vivo imaging systems. Ex-vivo digital autoradiography using a scintillator in combination with an image intensifier and a charged coupled device (CCD) has gained interest for pre-clinical ex-vivo alpha particle imaging. We present a simulation-based analysis of the fundamental spatial resolution limits of digital autoradiography systems. Spatial resolution was quantified in terms of the modulation transfer function (MTF) and Wagner's equivalent aperture. We modeled systems operating in either particle-counting (PC) or energy-integrating (EI) mode using a cascaded systems approach that accounts for: 1) the stopping power of alpha particles; 2) the distance alpha particles travel within the scintillator; 3) optical blur, and; 4) binning in detector elements. We applied our analysis to imaging of astatine-211 using an LYSO scintillator with thickness ranging from 10 μm to 20 μm. Our analysis demonstrates that when these systems are operated in particle-counting mode with a centroid-calculation algorithm, the effective apertures of 35 μm can be achieved, which suggests that digital autoradiography may enable quantifying the uptake of alpha emitters in tumors consisting of a few cancer cells. Future work will investigate the image noise and energy-resolution properties of digital autoradiography systems.

Clustering of galaxies near damped Lyman-alpha systems with (z) = 2.6

NASA Technical Reports Server (NTRS)

Wolfe, A. M

1993-01-01

The galaxy two-point correlation function, xi, at (z) = 2.6 is determined by comparing the number of Ly-alpha-emitting galaxies in narrowband CCD fields selected for the presence of damped L-alpha absorption to their number in randomly selected control fields. Comparisons between the presented determination of (xi), a density-weighted volume average of xi, and model predictions for (xi) at large redshifts show that models in which the clustering pattern is fixed in proper coordinates are highly unlikely, while better agreement is obtained if the clustering pattern is fixed in comoving coordinates. Therefore, clustering of Ly-alpha-emitting galaxies around damped Ly-alpha systems at large redshifts is strong. It is concluded that the faint blue galaxies are drawn from a parent population different from normal galaxies, the presumed offspring of damped Ly-alpha systems.

Galloylated polyphenols efficiently reduce alpha-tocopherol radicals in a phospholipid model system composed of sodium dodecyl sulfate (SDS) micelles.

PubMed

Pazos, Manuel; Torres, Josep Lluís; Andersen, Mogens L; Skibsted, Leif H; Medina, Isabel

2009-06-10

The ability of several polyphenolic fractions from grape ( Vitis vinifera ) pomace, pine ( Pinus pinaster ) bark, and witch hazel ( Hammamelis virginiana ) bark to repair alpha-tocopherol (alpha-TOH) through reduction of the alpha-tocopheroxyl radical was investigated in a homogeneous hexane system and a phospholipid-like system based on SDS micelles. These natural polyphenols were compared with pure related phenolics (epicatechin, gallic acid, epigallocatechin gallate, quercetin, and rutin) and ascorbic acid, which is a substance with a well-recognized capacity for regenerating alpha-TOH. alpha-Tocopheroxyl radicals were monitored and quantified by electron spin resonance (ESR) spectroscopy in the absence and presence of phenolics. Polyphenols from grape and pine bark were essentially catechin monomers and proanthocyanidins differing in the content of galloyl residues; those from pine bark had a negligible degree of galloylation. Polyphenolic fractions from witch hazel bark were composed of approximately 80% hydrolyzable tannins rich in galloyl moieties, together with a smaller amount of catechin monomers and proanthocyanidins. In the homogeneous hexane system, polyphenols from grape and pine bark exhibited similar activities, reducing the alpha-tocopheroxyl radicals by over 27-40%, whereas phenols from witch hazel were more highly effective, reducing 80% of alpha-TOH. In contrast, pine bark polyphenols were found to be significantly less active than the grape fractions in SDS micelles, reducing 30 and 70% of alpha-tocopheroxyl radicals, respectively. Polyphenolic fractions from witch hazel were also able to reduce the highest amount of alpha-TOH in SDS-micelles. The reducing capacity on alpha-tocopheroxyl radical of polyphenolic fractions was found to be pH-dependent and more effective at higher pH in the range of pH studied (5.8-7.8). These results stress the potential role of polyphenols, in particular those rich in galloyl groups, to maintain intact endogenous alpha-TOH in biological membranes through reduction of alpha-tocopheroxyl radicals.

Anomalous heat conduction and anomalous diffusion in nonlinear lattices, single walled nanotubes, and billiard gas channels.

PubMed

Li, Baowen; Wang, Jiao; Wang, Lei; Zhang, Gang

2005-03-01

We study anomalous heat conduction and anomalous diffusion in low-dimensional systems ranging from nonlinear lattices, single walled carbon nanotubes, to billiard gas channels. We find that in all discussed systems, the anomalous heat conductivity can be connected with the anomalous diffusion, namely, if energy diffusion is sigma(2)(t)=2Dt(alpha) (01) implies an anomalous heat conduction with a divergent thermal conductivity (beta>0), and more interestingly, a subdiffusion (alpha<1) implies an anomalous heat conduction with a convergent thermal conductivity (beta<0), consequently, the system is a thermal insulator in the thermodynamic limit. Existing numerical data support our theoretical prediction.

INFRARED SPECTROSCOPY OF SYMBIOTIC STARS. X. ORBITS FOR THREE S-TYPE SYSTEMS: V1044 CENTAURI, HEN 3-1213, AND SS 73-96

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fekel, Francis C.; Hinkle, Kenneth H.; Joyce, Richard R.

Employing new infrared radial velocities, we have computed orbits of the cool giants in three southern S-type symbiotic systems. The orbit for V1044 Cen, an M5.5 giant, has a period of 985 days and a modest eccentricity of 0.16. Hen 3-1213 is a K4 giant, yellow symbiotic with an orbital period of 533 days and a similar eccentricity of 0.18. For the M2 giant SS 73-96 the orbital period is 828 days, and this system has a somewhat larger eccentricity of 0.26. Measurement of the H i Paschen δ emission lines, which may at least partially reflect the motion ofmore » the secondary in SS 73-96, results in a mass ratio of 2.4 for the M giant relative to the presumed white dwarf. The estimated orbital inclinations of V1044 Cen and Hen 3-1213 are low, about 40°. However, for SS 73-96 the predicted inclination is 90°, and so an ephemeris for eclipses of the secondary or the hot nebula surrounding it is provided. A search of the orbital velocity residuals of V1044 Cen and SS 73-96 for pulsation periods produced no realistic or convincing period for either star.« less

Period Change Similarities Among the RR Lyrae Variables in Oosterhoff I and Oosterhoff II Globular Systems

NASA Astrophysics Data System (ADS)

Kunder, Andrea; Walker, Alistair; Stetson, Peter B.; Bono, Giuseppe; Nemec, James M.; de Propris, Roberto; Monelli, Matteo; Cassisi, Santi; Andreuzzi, Gloria; Dall'Ora, Massimo; Di Cecco, Alessandra; Zoccali, Manuela

2011-01-01

We present period change rates (dP/dt) for 42 RR Lyrae variables in the globular cluster IC 4499. Despite clear evidence of these period increases or decreases, the observed period change rates are an order of magnitude larger than predicted from theoretical models of this cluster. We find that there is a preference for increasing periods, a phenomenon observed in most RR Lyrae stars in Milky Way globular clusters. The period change rates as a function of position in the period-amplitude plane are used to examine possible evolutionary effects in OoI clusters, OoII clusters, field RR Lyrae stars, and the mixed-population cluster ω Centauri. It is found that there is no correlation between the period change rate and the typical definition of Oosterhoff groups. If the RR Lyrae period changes correspond with evolutionary effects, this would be in contrast to the hypothesis that RR Lyrae variables in OoII systems are evolved horizontal-branch stars that spent their zero-age horizontal-branch phase on the blue side of the instability strip. This may suggest that age may not be the primary explanation for the Oosterhoff types. Based in part on observations made with the European Southern Observatory telescopes obtained from the ESO/ST-ECF Science Archive Facility.

Nova Centauri 2013 = PNV J13544700-5909080

NASA Astrophysics Data System (ADS)

Waagen, Elizabeth O.

2013-12-01

Announces the discovery of V1369 Cen = Nova Cen 2013 = PNV J13544700-5909080 by John Seach (Chatsworth Island, NSW, Australia) at unfiltered magnitude 5.5 on 2013 December 02.692 UT. Low-resolution spectra obtained by Locke on Dec. 03.3776 UT and by Kaufman on Dec. 03.621 UT show strong Ha and Hb emission lines, indicating the object is a nova. Announced on IAU CBAT Central Bureau Electronic Telegram 3732 (Daniel W. E. Green, ed.). Finder charts with sequences may be created using the AAVSO Variable Star Plotter (http://www.aavso.org/vsp). Observations should be submitted to the AAVSO International Database. See full Alert Notice for more details and observations.

Inverted micellar intermediates and the transitions between lamellar, cubic, and inverted hexagonal lipid phases. II. Implications for membrane-membrane interactions and membrane fusion.

PubMed Central

Siegel, D P

1986-01-01

Results of a kinetic model of thermotropic L alpha----HII phase transitions are used to predict the types and order-of-magnitude rates of interactions between unilamellar vesicles that can occur by intermediates in the L alpha----HII phase transition. These interactions are: outer monolayer lipid exchange between vesicles; vesicle leakage subsequent to aggregation; and (only in systems with ratios of L alpha and HII phase structural dimensions in a certain range or with unusually large bilayer lateral compressibilities) vesicle fusion with retention of contents. It was previously proposed that inverted micellar structures mediate membrane fusion. These inverted micellar structures are thought to form in all systems with such transitions. However, I show that membrane fusion probably occurs via structures that form from these inverted micellar intermediates, and that fusion should occur in only a sub-set of lipid systems that can adopt the HII phase. For single-component phosphatidylethanolamine (PE) systems with thermotropic L alpha----HII transitions, lipid exchange should be observed starting at temperatures several degrees below TH and at all higher temperatures, where TH is the L alpha----HII transition temperature. At temperatures above TH, the HII phase forms between apposed vesicles, and eventually ruptures them (leakage). In most single-component PE systems, fusion via L alpha----HII transition intermediates should not occur. This is the behavior observed by Bentz, Ellens, Lai, Szoka, et al. in PE vesicle systems. Fusion is likely to occur under circumstances in which multilamellar samples of lipid form the so-called "inverted cubic" or "isotropic" phase. This is as observed in the mono-methyl DOPE system (Ellens, H., J. Bentz, and F. C. Szoka. 1986. Fusion of phosphatidylethanolamine containing liposomes and the mechanism of the L alpha-HII phase transition. Biochemistry. In press.) In lipid systems with L alpha----HII transitions driven by cation binding (e.g., Ca2+-cardiolipin), fusion should be more frequent than in thermotropic systems. PMID:3719075

The X-43A Flush Airdata Sensing System Flight Test Results

NASA Technical Reports Server (NTRS)

Baumann, Ethan; Pahle, Joseph W.; Davis, Mark; White, John Terry

2008-01-01

The National Aeronautics and Space Administration (NASA) has flight-tested a flush airdata sensing (FADS) system on the Hyper-X Research Vehicle (X-43A) at hypersonic speeds during the course of two successful flights. For this series of tests, the FADS system was calibrated to operate between Mach 3 and Mach 8, and flight test data was collected between Mach 1 and Mach 10. The FADS system acquired pressure data from surface-mounted ports and generated a real-time angle-of-attack (alpha) estimate on board the X-43A. The collected data were primarily intended to evaluate the FADS system performance, and the estimated alpha was used by the flight control algorithms on the X-43A for only a portion of the first successful flight. This paper provides an overview of the FADS system and alpha estimation algorithms, presents the in-flight alpha estimation algorithm performance, and provides comparisons to wind tunnel results and theory. Results indicate that the FADS system adequately estimated the alpha of the vehicle during the hypersonic portions of the two flights.

The Interstellar Conspiracy

NASA Technical Reports Server (NTRS)

Johnson, Les; Matloff, Gregory L.

2005-01-01

If we were designing a human-carrying starship that could be launched in the not-too-distant future, it would almost certainly not use a warp drive to instantaneously bounce around the universe, as is done in Isaac Asimov's classic Foundation series or in episodes of Star Trek or Star Wars. Sadly, those starships that seem to be within technological reach could not even travel at high relativistic speeds, as does the interstellar ramjet in Poul Anderson's Tau Zero. Warp-speeds seem to be well outside the realm of currently understood physical law; proton-fusing ramjets may never be technologically feasible. Perhaps fortunately in our terrorist-plagued world, the economics of antimatter may never be attractive for large-scale starship propulsion. But interstellar travel will be possible within a few centuries, although it will certainly not be as fast as we might prefer. If humans learn how to hibernate, perhaps we will sleep our way to the stars, as do the crew in A. E. van Vogt's Far Centaurus. However, as discussed in a landmark paper in The Journal of the British Interplanetary Society, the most feasible approach to transporting a small human population to the planets (if any) of Alpha Centauri is the worldship. Such craft have often been featured in science fiction. See for example Arthur C. Clarke's Rendezvous with Rama, and Robert A. Heinlein's Orphans of the Sky. Worldships are essentially mobile versions of the O Neill free-space habitats. Constructed mostly from lunar and/or asteroidal materials, these solar-powered, multi-kilometer-dimension structures could house 10,000 to 100,000 humans in Earth-approximating environments. Artificial gravity would be provided by habitat rotation, and cosmic ray shielding would be provided by passive methods, such as habitat atmosphere and mass shielding, or magnetic fields. A late 21st century space-habitat venture might support itself economically by constructing large solar-powered satellites to beam energy back to Earth.

Permanent renal loss following tumor necrosis factor α antagonists for arthritis.

PubMed

Chen, Tzu-Jen; Yang, Ya-Fei; Huang, Po-Hao; Lin, Hsin-Hung; Huang, Chiu-Ching

2010-06-01

Tumor necrosis factor alpha (TNF-alpha) antagonists are now widely used in the treatment of aggressive rheumatoid arthritis and are generally well tolerated. Although rare, they could induce systemic lupus erythematosus, glomerulonephritis, and antineutrophil cytoplasmic antibody associated systemic vasculitis. Tumor necrosis factor alpha antagonists associated glomerulonephritis usually subsides after discontinuation of the therapy and subsequent initiation of corticosteroids and immunosuppressive agents. Here we describe crescentic glomerulonephritis progression to end-stage renal disease in a patient following two doses of TNF-alpha antagonists for the treatment of reactive arthritis. To our knowledge, dialysis dependent permanent renal loss after TNF-alpha antagonists has not yet been reported. We suggest the renal function should be closely monitored in patients treated with TNF-alpha antagonists by rheumatologists.

Alpha Rhythms in Audition: Cognitive and Clinical Perspectives

PubMed Central

Weisz, Nathan; Hartmann, Thomas; Müller, Nadia; Lorenz, Isabel; Obleser, Jonas

2011-01-01

Like the visual and the sensorimotor systems, the auditory system exhibits pronounced alpha-like resting oscillatory activity. Due to the relatively small spatial extent of auditory cortical areas, this rhythmic activity is less obvious and frequently masked by non-auditory alpha-generators when recording non-invasively using magnetoencephalography (MEG) or electroencephalography (EEG). Following stimulation with sounds, marked desynchronizations can be observed between 6 and 12 Hz, which can be localized to the auditory cortex. However knowledge about the functional relevance of the auditory alpha rhythm has remained scarce so far. Results from the visual and sensorimotor system have fuelled the hypothesis of alpha activity reflecting a state of functional inhibition. The current article pursues several intentions: (1) Firstly we review and present own evidence (MEG, EEG, sEEG) for the existence of an auditory alpha-like rhythm independent of visual or motor generators, something that is occasionally met with skepticism. (2) In a second part we will discuss tinnitus and how this audiological symptom may relate to reduced background alpha. The clinical part will give an introduction into a method which aims to modulate neurophysiological activity hypothesized to underlie this distressing disorder. Using neurofeedback, one is able to directly target relevant oscillatory activity. Preliminary data point to a high potential of this approach for treating tinnitus. (3) Finally, in a cognitive neuroscientific part we will show that auditory alpha is modulated by anticipation/expectations with and without auditory stimulation. We will also introduce ideas and initial evidence that alpha oscillations are involved in the most complex capability of the auditory system, namely speech perception. The evidence presented in this article corroborates findings from other modalities, indicating that alpha-like activity functionally has an universal inhibitory role across sensory modalities. PMID:21687444

Antioxidant status and alpha1-antiproteinase activity in subarachnoid hemorrhage patients.

PubMed

Marzatico, F; Gaetani, P; Tartara, F; Bertorelli, L; Feletti, F; Adinolfi, D; Tancioni, F; Rodriguez y Baena, R

1998-01-01

The antiproteasic activity of alpha1-antitrypsin (alpha1-AT) is reduced in cases of subarachnoid hemorrhage from ruptured intracranial aneurysm and particularly in patients currently smoking; alpha1-AT is very sensitive to oxidant agents. About 50% of physiological anti-oxidant systemic capacity is represented by Vitamin A, E and C. Plasmatic amounts of alpha1-AT, alpha1-AT Collagenase Inhibitory Capacity (CIC) and levels of vitamin A, vitamin E and vitamin C were analyzed in 39 patients, 26 women and 13 men, operated for intracranial aneurysm; 11 patients with unruptured intracranial aneurysm were considered as controls while 28 patients were included within 12 hours from subarachnoid hemorrhage (SAH). Plasmatic levels of vitamin A and vitamin E were significantly lower (p=0.038 and p=0.0158) in patients suffering SAH than in controls, while no statistically significant differences were found in mean plasmatic vitamin C levels. Level of alpha1-AT was not statistically different in controls and in patients with SAH; however, the activity of alpha1-AT, evaluated as CIC, is significantly reduced in patients with SAH (p=0.019). We have observed that systemic plasmatic levels of vitamins did not significantly differ in relation to smoking habit. Vitamin A and E represent an important defensive system against free radicals reactions. Particularly, vitamin E acts as an antioxidant by scavenging free-radicals. A reduced anti-oxidant status might be related to the higher sensibility of alpha1-AT to oxidative reactions and the activity of alpha1-AT is dependent on the antioxidant capacity of liposoluble vitamins. We can speculate that an acute systemic oxidative stress condition might influence the rupture of intracranial aneurysms.

Alpha: A real-time decentralized operating system for mission-oriented system integration and operation

NASA Technical Reports Server (NTRS)

Jensen, E. Douglas

1988-01-01

Alpha is a new kind of operating system that is unique in two highly significant ways. First, it is decentralized transparently providing reliable resource management across physically dispersed nodes, so that distributed applications programming can be done largely as though it were centralized. And second, it provides comprehensive, high technology support for real-time system integration and operation, an application area which consists predominately of aperiodic activities having critical time constraints such as deadlines. Alpha is extremely adaptable so that it can be easily optimized for a wide range of problem-specific functionality, performance, and cost. Alpha is the first systems effort of the Archons Project, and the prototype was created at Carnegie-Mellon University directly on modified Sun multiprocessor workstation hardware. It has been demonstrated with a real-time C(sup 2) application. Continuing research is leading to a series of enhanced follow-ons to Alpha; these are portable but initially hosted on Concurrent's MASSCOMP line of multiprocessor products.

40 CFR 141.26 - Monitoring frequency and compliance requirements for radionuclides in community water systems.

Code of Federal Regulations, 2012 CFR

2012-07-01

.... For the purposes of monitoring for gross alpha particle activity, radium-226, radium-228, uranium, and... monitoring: Systems must conduct initial monitoring for gross alpha particle activity, radium-226, radium-228...) For gross alpha particle activity, uranium, radium-226, and radium-228 monitoring, the State may waive...

21 CFR 866.5420 - Alpha-1-glycoproteins immunological test system.

Code of Federal Regulations, 2014 CFR

2014-04-01

... electrophoresis) in serum and other body fluids. Measurement of specific alpha-1-glycoproteins may aid in the... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Alpha-1-glycoproteins immunological test system. 866.5420 Section 866.5420 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5130 - Alpha-1-antitrypsin immunological test system.

Code of Federal Regulations, 2012 CFR

2012-04-01

... the alpha-1-antitrypsin (a plasma protein) in serum, other body fluids, and tissues. The measurements... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Alpha-1-antitrypsin immunological test system. 866.5130 Section 866.5130 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5130 - Alpha-1-antitrypsin immunological test system.

Code of Federal Regulations, 2014 CFR

2014-04-01

... the alpha-1-antitrypsin (a plasma protein) in serum, other body fluids, and tissues. The measurements... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Alpha-1-antitrypsin immunological test system. 866.5130 Section 866.5130 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5420 - Alpha-1-glycoproteins immunological test system.

Code of Federal Regulations, 2013 CFR

2013-04-01

... electrophoresis) in serum and other body fluids. Measurement of specific alpha-1-glycoproteins may aid in the... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Alpha-1-glycoproteins immunological test system. 866.5420 Section 866.5420 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5130 - Alpha-1-antitrypsin immunological test system.

Code of Federal Regulations, 2013 CFR

2013-04-01

... the alpha-1-antitrypsin (a plasma protein) in serum, other body fluids, and tissues. The measurements... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Alpha-1-antitrypsin immunological test system. 866.5130 Section 866.5130 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5420 - Alpha-1-glycoproteins immunological test system.

Code of Federal Regulations, 2012 CFR

2012-04-01

... electrophoresis) in serum and other body fluids. Measurement of specific alpha-1-glycoproteins may aid in the... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Alpha-1-glycoproteins immunological test system. 866.5420 Section 866.5420 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5130 - Alpha-1-antitrypsin immunological test system.

Code of Federal Regulations, 2011 CFR

2011-04-01

... the alpha-1-antitrypsin (a plasma protein) in serum, other body fluids, and tissues. The measurements... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Alpha-1-antitrypsin immunological test system. 866.5130 Section 866.5130 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

21 CFR 866.5420 - Alpha-1-glycoproteins immunological test system.

Code of Federal Regulations, 2011 CFR

2011-04-01

... electrophoresis) in serum and other body fluids. Measurement of specific alpha-1-glycoproteins may aid in the... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Alpha-1-glycoproteins immunological test system. 866.5420 Section 866.5420 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN...

GALEX Grism Spectroscopy of the Globular Cluster Omega Centauri

NASA Astrophysics Data System (ADS)

Sweigart, Allen

We propose to obtain GALEX FUV-only grism spectroscopy of the hot stars in omega Centauri, the most massive globular cluster in our Galaxy. Previous UIT imagery of omega Cen showed that it contains about 2000 hot horizontal branch (HB) stars, and we estimate that GALEX spectra can be obtained for about 500 of these stars in the outer regions of the cluster, including about 50 of the hot ``blue hook'' stars discovered with UIT. The blue hook stars appear to be both hotter (35,000 K) and less luminous in the UIT color-magnitude diagram than predicted by canonical HB models and, indeed, are unexplained by standard evolutionary theory. Brown et al. (2001) have suggested that the blue hook stars are the progeny of stars which mixed their surface hydrogen into their hot He-burning interior during a delayed helium flash subsequent to leaving the red giant branch. This ``flash-mixing'' results in a hot hydrogen-deficient star with a typical surface abundance of 96% He and 4% C by mass. The GALEX spectral region includes the strong lines of C III 1426, 1578 A, C IV 1550 A, and He II 1640 A which will allow this predicted carbon and helium enrichment to be detected. These observations will therefore provide a crucial test of the Brown et al. flash-mixing hypothesis and will determine if flash mixing represents a new evolutionary channel for populating the hot HB. The GALEX spectra will also address other questions concerning the hot HB in omega Cen including (1) the metallicity distribution of HB stars with 9,000 K < Teff < 11,000 K, (2) the effect of radiative levitation on the UV spectra of stars with Teff > 11,000 K, and (3) the origin of the subluminous HB stars found in the UIT photometry with 15,000K < Teff < 30,000 K.

Augmentation of the noradrenergic system in alpha-2 adrenergic receptor deficient mice: anatomical changes associated with enhanced fear memory.

PubMed

Davies, M Frances; Tsui, Janet Y; Flannery, Judy A; Li, Xiangqi; DeLorey, Timothy M; Hoffman, Brian B

2003-10-03

We have investigated sensitivity to the conditioned fear procedure of mice is influenced by the genetic deletion of alpha2A adrenoceptors (ARs). We observed a heightened freezing response in the discrete cue memory test in alpha2A AR knockout (alpha2A AR KO) mice and in D79N mice, a transgenic mouse strain with functionally impaired alpha2A ARs. No significant differences in contextual memory were observed between control and alpha2A AR KO or D79N mice suggesting a minimal role for the noradrenergic system in contextual memory. We speculated that the increased freezing response of the alpha2A AR KO and D79N mice in the discrete cue setting was due to increased release of norepinephrine evoked by the unconditioned footshock stimulus. In alpha2A AR KO mice we measured a doubling in the number of noradrenergic neurons in the locus coeruleus (LC) and a large increase in the cell volume of tyrosine hydroxylase positive neurons, likely due to selective preservation of large, multipolar neurons in the subcoeruleus. Hyperplasia of the noradrenergic neurons in the nucleus tractus solitarius, A5 and A7, was also observed. Alpha2A AR KO mice exhibit greater c-Fos expression in the LC compared to wild type mice suggesting that the LC neurons in the alpha2A AR KO mice were spontaneously more active. This study suggests that alpha2A ARs are involved in the development of the central noradrenergic system and raises the possibility that alterations in alpha2A AR expression may contribute to variations in fear and stress responses.

Top-down controlled alpha band activity in somatosensory areas determines behavioral performance in a discrimination task.

PubMed

Haegens, Saskia; Händel, Barbara F; Jensen, Ole

2011-04-06

The brain receives a rich flow of information which must be processed according to behavioral relevance. How is the state of the sensory system adjusted to up- or downregulate processing according to anticipation? We used magnetoencephalography to investigate whether prestimulus alpha band activity (8-14 Hz) reflects allocation of attentional resources in the human somatosensory system. Subjects performed a tactile discrimination task where a visual cue directed attention to their right or left hand. The strength of attentional modulation was controlled by varying the reliability of the cue in three experimental blocks (100%, 75%, or 50% valid cueing). While somatosensory prestimulus alpha power lateralized strongly with a fully predictive cue (100%), lateralization was decreased with lower cue reliability (75%) and virtually absent if the cue had no predictive value at all (50%). Importantly, alpha lateralization influenced the subjects' behavioral performance positively: both accuracy and speed of response improved with the degree of alpha lateralization. This study demonstrates that prestimulus alpha lateralization in the somatosensory system behaves similarly to posterior alpha activity observed in visual attention tasks. Our findings extend the notion that alpha band activity is involved in shaping the functional architecture of the working brain by determining both the engagement and disengagement of specific regions: the degree of anticipation modulates the alpha activity in sensory regions in a graded manner. Thus, the alpha activity is under top-down control and seems to play an important role for setting the state of sensory regions to optimize processing.

Brain-computer interface design using alpha wave

NASA Astrophysics Data System (ADS)

Zhao, Hai-bin; Wang, Hong; Liu, Chong; Li, Chun-sheng

2010-01-01

A brain-computer interface (BCI) is a novel communication system that translates brain activity into commands for a computer or other electronic devices. BCI system based on non-invasive scalp electroencephalogram (EEG) has become a hot research area in recent years. BCI technology can help improve the quality of life and restore function for people with severe motor disabilities. In this study, we design a real-time asynchronous BCI system using Alpha wave. The basic theory of this BCI system is alpha wave-block phenomenon. Alpha wave is the most prominent wave in the whole realm of brain activity. This system includes data acquisition, feature selection and classification. The subject can use this system easily and freely choose anyone of four commands with only short-time training. The results of the experiment show that this BCI system has high classification accuracy, and has potential application for clinical engineering and is valuable for further research.

Convergence of Distributed Optimal Controls on the Internal Energy in Mixed Elliptic Problems when the Heat Transfer Coefficient Goes to Infinity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gariboldi, C.; E-mail: cgariboldi@exa.unrc.edu.ar; Tarzia, D.

2003-05-21

We consider a steady-state heat conduction problem P{sub {alpha}} with mixed boundary conditions for the Poisson equation depending on a positive parameter {alpha} , which represents the heat transfer coefficient on a portion {gamma} {sub 1} of the boundary of a given bounded domain in R{sup n} . We formulate distributed optimal control problems over the internal energy g for each {alpha}. We prove that the optimal control g{sub o}p{sub {alpha}} and its corresponding system u{sub go}p{sub {alpha}}{sub {alpha}} and adjoint p{sub go}p{sub {alpha}}{sub {alpha}} states for each {alpha} are strongly convergent to g{sub op},u{sub gop} and p{sub gop} ,more » respectively, in adequate functional spaces. We also prove that these limit functions are respectively the optimal control, and the system and adjoint states corresponding to another distributed optimal control problem for the same Poisson equation with a different boundary condition on the portion {gamma}{sub 1} . We use the fixed point and elliptic variational inequality theories.« less

Study on molecular epidemiology of the alpha-thalassemias in Liuzhou City, Guangxi Autonomous Region, China.

PubMed

Cai, Ren; Liu, Jingzhong; Wang, Lirong; Liang, Xin; Xiao, Bai; Su, Liu; Zhou, Yan; Pan, Lizhen

2004-01-01

Guangxi is one of the provinces of Southern China with the highest incidence of alpha-thalassemia (thal). Liuzhou is the second biggest city in Guangxi. To find out the incidence of the various alpha-thal genotypes, and their distribution in the Liuzhou area, an F820 Blood Cell Analysis System was used to measure the parameters of red blood cells. A SPIFE Rapid Auto-Electrophoresis System was used to analyze the normal and abnormal hemoglobins (Hbs). Multiplex polymerase chain reaction (mPCR) was used to detect the alpha-globin genotypes. Thirty-two (2.05%) out of 7805 young couples undergoing pre-marriage counseling, were diagnosed as having an Hb H (beta4) disease. The study of 1228 cord blood samples revealed 138 newborn children carrying an alpha-thal determinant with nine different genotypes, thus making the total incidence of alpha-thal 11.24%. Among 185 cases of Hb H, 119 (64.1%) were confirmed as being deletional, and 66 cases (35.7%) nondeletional types. The severity of the Hb H diseases could be classified in the following order: alphaCSalpha/--SEA (alphaConstant (Spring)alpha/--Southeast Asia); alpha(-4.2)/--SEA; alpha(-3.7)/--SEA. Ten cases of alpha-thal determinants were found in combination with beta-thal. The mPCR technique can detect all kinds of combinations of the three common large deletions (--SEA, alpha(-4.2) and alpha(-3.7)) accurately and conveniently.

Gap-minimal systems of notations and the constructible hierarchy

NASA Technical Reports Server (NTRS)

Lucian, M. L.

1972-01-01

If a constructibly countable ordinal alpha is a gap ordinal, then the order type of the set of index ordinals smaller than alpha is exactly alpha. The gap ordinals are the only points of discontinuity of a certain ordinal-valued function. The notion of gap minimality for well ordered systems of notations is defined, and the existence of gap-minimal systems of notations of arbitrarily large constructibly countable length is established.

Survey of Portions of the Chromium-Cobalt-Nickel-Molybdenum Quaternary System at 1,200 Degrees C

NASA Technical Reports Server (NTRS)

Rideout, Sheldon Paul; Beck, Paul A

1953-01-01

A survey was made of portions of the chromium-cobalt-nickel-molybdenum quaternary system at 1,200 degrees c by means of microscopic and x-ray diffraction studies. Since the face-centered cubic (alpha) solid solutions form the matrix of almost all practically useful high-temperature alloys, the solid solubility limits of the quaternary alpha phase were determined up to 20 percent molybdenum. The component cobalt-nickel-molybdenum, chromium-cobalt-molybdenum, and chromium-nickel-molybdenum ternary systems were also studied. The survey of these systems was confined to the determination of the boundaries of the face-centered cubic (alpha) solid solutions and of the phases coexisting with alpha at 1,200 degrees c.

Further evidence for cosmological evolution of the fine structure constant.

PubMed

Webb, J K; Murphy, M T; Flambaum, V V; Dzuba, V A; Barrow, J D; Churchill, C W; Prochaska, J X; Wolfe, A M

2001-08-27

We describe the results of a search for time variability of the fine structure constant alpha using absorption systems in the spectra of distant quasars. Three large optical data sets and two 21 cm and mm absorption systems provide four independent samples, spanning approximately 23% to 87% of the age of the universe. Each sample yields a smaller alpha in the past and the optical sample shows a 4 sigma deviation: Delta alpha/alpha = -0.72+/-0.18 x 10(-5) over the redshift range 0.5

Evaluation of the alpha-1 and alpha-2 adrenoceptor-mediated effects of a series of dimethoxy-substituted tolazoline derivatives in the cardiovascular system of the pithed rat.

PubMed

Ruffolo, R R; Messick, K

1985-01-01

The alpha-1 and alpha-2 adrenoceptor-mediated effects of a series of dimethoxy-substituted tolazoline derivatives were investigated in the cardiovascular system of the pithed rat. The 2,5- and 3,5-dimethoxy-substituted tolazoline derivatives produced vasopressor responses that were inhibited by the alpha-1 adrenoceptor antagonist, prazosin (0.1 mg/kg i.v.), and were not affected by the alpha-2 adrenoceptor antagonist, yohimbine (1 mg/kg i.v.), suggesting that these derivatives selectively activate postsynaptic vascular alpha-1 adrenoceptors. The 2,5- and 3,5-dimethoxy-substituted derivatives of tolazoline did not produce an alpha-2 adrenoceptor-mediated inhibition of neurogenic tachycardia in cord-stimulated pithed rats and were therefore presumed to be devoid of alpha-2 adrenoceptor agonist activity. In contrast, 2,3-dimethoxytolazoline produced a vasopressor effect that was inhibited by yohimbine but not by prazosin, suggesting selective activation of postsynaptic vascular alpha-2 adrenoceptors. Consistent with this observation is the fact that 2,3-dimethoxytolazoline elicited a dose-dependent, alpha-2 adrenoceptor-mediated inhibition of neurogenic tachycardia in cord-stimulated pithed rat. 3,4-Dimethoxytolazoline was a weak alpha-1 adrenoceptor agonist in the vasculature of the pithed rat and was devoid of agonist activity at alpha-2 adrenoceptors. However, 3,4-dimethoxytolazoline was found to be an alpha-2 adrenoceptor antagonist of similar potency as yohimbine. The results of the present study indicate that dimethoxy-substituted derivatives of tolazoline possess different activities and selectivities at alpha-1 and alpha-2 adrenoceptors depending upon the positions of substitution.(ABSTRACT TRUNCATED AT 250 WORDS)

21 CFR 866.5080 - Alpha-1-antichymotrypsin immunological test system.

Code of Federal Regulations, 2011 CFR

2011-04-01

... immunochemical techniques alpha-1-antichymotrypsin (a protein) in serum, other body fluids, and tissues. Alpha-1-antichymotrypsin helps protect tissues against proteolytic (protein-splitting) enzymes released during infection...

Massive pulsating stars observed by BRITE-Constellation. I. The triple system β Centauri (Agena)

NASA Astrophysics Data System (ADS)

Pigulski, A.; Cugier, H.; Popowicz, A.; Kuschnig, R.; Moffat, A. F. J.; Rucinski, S. M.; Schwarzenberg-Czerny, A.; Weiss, W. W.; Handler, G.; Wade, G. A.; Koudelka, O.; Matthews, J. M.; Mochnacki, St.; Orleański, P.; Pablo, H.; Ramiaramanantsoa, T.; Whittaker, G.; Zocłońska, E.; Zwintz, K.

2016-04-01

Context. Asteroseismology of massive pulsating stars of β Cep and SPB types can help us to uncover the internal structure of massive stars and understand certain physical phenomena that are taking place in their interiors. We study β Centauri (Agena), a triple system with two massive fast-rotating early B-type components which show p- and g-mode pulsations; the system's secondary is also known to have a measurable magnetic field. Aims: This paper aims to precisely determine the masses and detect pulsation modes in the two massive components of β Cen with BRITE-Constellation photometry. In addition, seismic models for the components are considered and the effects of fast rotation are discussed. This is done to test the limitations of seismic modeling for this very difficult case. Methods: A simultaneous fit of visual and spectroscopic orbits is used to self-consistently derive the orbital parameters, and subsequently the masses, of the components. Time-series analysis of BRITE-Constellation data is used to detect pulsation modes and derive their frequencies, amplitudes, phases, and rates of frequency change. Theoretically-predicted frequencies are calculated for the appropriate evolutionary models and their stability is checked. The effects of rotational splitting and coupling are also presented. Results: The derived masses of the two massive components are equal to 12.02 ± 0.13 and 10.58 ± 0.18 M⊙. The parameters of the wider, A-B system, presently approaching periastron passage, are constrained. Analysis of the combined blue- and red-filter BRITE-Constellation photometric data of the system revealed the presence of 19 periodic terms, of which eight are likely g modes, nine are p modes, and the remaining two are combination terms. It cannot be excluded that one or two low-frequency terms are rotational frequencies. It is possible that both components of β Cen are β Cep/SPB hybrids. An attempt to use the apparent changes of frequency to distinguish which modes originate in which component did not succeed, but there is potential for using this method when more BRITE data become available. Conclusions: Agena seems to be one of very few rapidly rotating massive objects with rich p- and g-mode spectra, and precisely known masses. It can therefore be used to gain a better understanding of the excitation of pulsations in relatively rapidly rotating stars and their seismic modeling. Lacking proper mode identification, the pulsation frequencies found in β Cen cannot yet be used to constrain the internal structure of the components, but it may be possible to achieve this in the future with the use of spectroscopy and spectropolarimetry. In particular, these kinds of data can be used for mode identification since they provide new radial velocities. In consequence, they may help to improve the orbital solution, derive more precise masses, magnetic field strength and geometry, inclination angles, and reveal rotation periods. They may also help to assign pulsation frequencies to components. Finally, the case studied here illustrates the potential of BRITE-Constellation data for the detection of rich-frequency spectra of small-amplitude modes in massive pulsating stars. Based on data collected by the BRITE-Constellation satellite mission, built, launched and operated thanks to support from the Austrian Aeronautics and Space Agency and the University of Vienna, the Canadian Space Agency (CSA) and the Foundation for Polish Science & Technology (FNiTP MNiSW) and National Centre for Science (NCN).

Peroxisome proliferator-activated receptor {alpha}-independent peroxisome proliferation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang Xiuguo; Tanaka, Naoki; Nakajima, Takero

2006-08-11

Hepatic peroxisome proliferation, increases in the numerical and volume density of peroxisomes, is believed to be closely related to peroxisome proliferator-activated receptor {alpha} (PPAR{alpha}) activation; however, it remains unknown whether peroxisome proliferation depends absolutely on this activation. To verify occurrence of PPAR{alpha}-independent peroxisome proliferation, fenofibrate treatment was used, which was expected to significantly enhance PPAR{alpha} dependence in the assay system. Surprisingly, a novel type of PPAR{alpha}-independent peroxisome proliferation and enlargement was uncovered in PPAR{alpha}-null mice. The increased expression of dynamin-like protein 1, but not peroxisome biogenesis factor 11{alpha}, might be associated with the PPAR{alpha}-independent peroxisome proliferation at least in part.

The p38alpha mitogen-activated protein kinase as a central nervous system drug discovery target.

PubMed

Borders, Aaron S; de Almeida, Lucia; Van Eldik, Linda J; Watterson, D Martin

2008-12-03

Protein kinases are critical modulators of a variety of cellular signal transduction pathways, and abnormal phosphorylation events can be a cause or contributor to disease progression in a variety of disorders. This has led to the emergence of protein kinases as an important new class of drug targets for small molecule therapeutics. A serine/threonine protein kinase, p38alpha mitogen-activated protein kinase (MAPK), is an established therapeutic target for peripheral inflammatory disorders because of its critical role in regulation of proinflammatory cytokine production. There is increasing evidence that p38alpha MAPK is also an important regulator of proinflammatory cytokine levels in the central nervous system, raising the possibility that the kinase may be a drug discovery target for central nervous system disorders where cytokine overproduction contributes to disease progression. Development of bioavailable, central nervous system-penetrant p38alpha MAPK inhibitors provides the required foundation for drug discovery campaigns targeting p38alpha MAPK in neurodegenerative disorders.

Rotational modulation and flares on RS Canum Venaticorum and BY Draconis-type stars. XV - Observations of Proxima Centauri and solar calibration data

NASA Technical Reports Server (NTRS)

Haisch, B. M.; Butler, C. J.; Foing, B.; Rodono, M.; Giampapa, M. S.

1990-01-01

Results are reported from simultaneous Exosat and IUE observations of flaring in Proxima Cen on March 2, 1985. The data are presented in extensive tables and sample spectra and discussed in detail. The peak emission of the soft-X-ray flare is found to be about 3 x 10 to the 27th erg/sec, with energy about 3 x 10 to the 30th erg and an associated increase in Mg II flux to 17,000-67,000 erg/sq cm sec, or about an order of magnitude lower than the solar value. The presence of flare- and microflare-related processes heating the corona is inferred.

Epidermal growth factor system is a physiological regulator of development of the mouse fetal submandibular gland and regulates expression of the alpha6-integrin subunit.

PubMed

Kashimata, M; Gresik, E W

1997-02-01

Epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) regulate branching morphogenesis of fetal mouse submandibular gland (SMG) rudiments in vitro. The EGF system (EGF, TGF-alpha, and their shared receptor, EGFR) also regulates expression of integrins and their ligands in the extracellular matrix. We show here that inhibition of EGFR tyrosine-kinase activity by a tyrphostin retards in vitro development of SMGs. Using total RNA isolated from pooled SMGs taken from intact mouse fetuses, mRNA transcripts for EGF, TGF-alpha, and EGFR were detected by reverse transcription-polymerase chain reaction (RT-PCR), and age-dependent variations in the levels of these mRNA were quantitatively determined by nuclease protection assays. These findings suggest that the EGF system is operative in the in vivo development of this gland. alpha6-Integrin subunit was localized by immunofluorescence at the basal surface of epithelial cells. Branching morphogenesis of cultured SMG rudiments was inhibited by anti-alpha6 antibodies. Synthesis of alpha6-subunit in cultured SMGs, detected by metabolic labeling and immunoprecipitation, was increased by EGF and drastically reduced by tyrphostin. RT-PCR revealed that mRNAs for alpha6- and beta1- and beta4-integrin subunits are expressed at all ages between embryonic day 13 and postnatal day 7. These findings suggest that 1) the EGF system is a physiologic regulator of development of fetal mouse SMG, and 2) one mechanism by which it acts may be by regulating expression of integrins, which in turn control interaction of epithelial cells with the extracellular matrix.

Riemannian geometry of thermodynamics and systems with repulsive power-law interactions.

PubMed

Ruppeiner, George

2005-07-01

A Riemannian geometric theory of thermodynamics based on the postulate that the curvature scalar R is proportional to the inverse free energy density is used to investigate three-dimensional fluid systems of identical classical point particles interacting with each other via a power-law potential energy gamma r(-alpha) . Such systems are useful in modeling melting transitions. The limit alpha-->infinity corresponds to the hard sphere gas. A thermodynamic limit exists only for short-range (alpha>3) and repulsive (gamma>0) interactions. The geometric theory solutions for given alpha>3 , gamma>0 , and any constant temperature T have the following properties: (1) the thermodynamics follows from a single function b (rho T(-3/alpha) ) , where rho is the density; (2) all solutions are equivalent up to a single scaling constant for rho T(-3/alpha) , related to gamma via the virial theorem; (3) at low density, solutions correspond to the ideal gas; (4) at high density there are solutions with pressure and energy depending on density as expected from solid state physics, though not with a Dulong-Petit heat capacity limit; (5) for 33.7913 a phase transition is required to go between these regimes; (7) for any alpha>3 we may include a first-order phase transition, which is expected from computer simulations; and (8) if alpha-->infinity, the density approaches a finite value as the pressure increases to infinity, with the pressure diverging logarithmically in the density difference.

All Source Analysis System (ASAS): Migration from VAX to Alpha AXP computer systems

NASA Technical Reports Server (NTRS)

Sjoholm-Sierchio, Michael J.; Friedman, Steven Z. (Editor)

1994-01-01

The Jet Propulsion Laboratory's (JPL's) experience migrating existing VAX applications to Digital Equipment Corporation's new Alpha AXP processor is covered. The rapid development approach used during the 10-month period required to migrate the All Source Analysis System (ASAS), 1.5 million lines of FORTRAN, C, and Ada code, is also covered. ASAS, an automated tactical intelligence system, was developed by the Jet Propulsion Laboratory for the U. S. Army. Other benefits achieved as a result of the significant performance improvements provided by Alpha AXP platform are also described.

Unitary scintillation detector and system

DOEpatents

McElhaney, Stephanie A.; Chiles, Marion M.

1994-01-01

The invention is a unitary alpha, beta, and gamma scintillation detector and system for sensing the presence of alpha, beta, and gamma radiations selectively or simultaneously. The scintillators are mounted in a light-tight housing provided with an entrance window for admitting alpha, beta, and gamma radiation and excluding ambient light from the housing. Light pulses from each scintillator have different decay constants that are converted by a photosensitive device into corresponding differently shaped electrical pulses. A pulse discrimination system identifies the electrical pulses by their respective pulse shapes which are determined by decay time. The identified electrical pulses are counted in separate channel analyzers to indicate the respective levels of sensed alpha, beta, and gamma radiations.

Unitary scintillation detector and system

DOEpatents

McElhaney, S.A.; Chiles, M.M.

1994-05-31

The invention is a unitary alpha, beta, and gamma scintillation detector and system for sensing the presence of alpha, beta, and gamma radiations selectively or simultaneously. The scintillators are mounted in a light-tight housing provided with an entrance window for admitting alpha, beta, and gamma radiation and excluding ambient light from the housing. Light pulses from each scintillator have different decay constants that are converted by a photosensitive device into corresponding differently shaped electrical pulses. A pulse discrimination system identifies the electrical pulses by their respective pulse shapes which are determined by decay time. The identified electrical pulses are counted in separate channel analyzers to indicate the respective levels of sensed alpha, beta, and gamma radiations. 10 figs.

Portable wireless neurofeedback system of EEG alpha rhythm enhances memory.

PubMed

Wei, Ting-Ying; Chang, Da-Wei; Liu, You-De; Liu, Chen-Wei; Young, Chung-Ping; Liang, Sheng-Fu; Shaw, Fu-Zen

2017-11-13

Effect of neurofeedback training (NFT) on enhancement of cognitive function or amelioration of clinical symptoms is inconclusive. The trainability of brain rhythm using a neurofeedback system is uncertainty because various experimental designs are used in previous studies. The current study aimed to develop a portable wireless NFT system for alpha rhythm and to validate effect of the NFT system on memory with a sham-controlled group. The proposed system contained an EEG signal analysis device and a smartphone with wireless Bluetooth low-energy technology. Instantaneous 1-s EEG power and contiguous 5-min EEG power throughout the training were developed as feedback information. The training performance and its progression were kept to boost usability of our device. Participants were blinded and randomly assigned into either the control group receiving random 4-Hz power or Alpha group receiving 8-12-Hz power. Working memory and episodic memory were assessed by the backward digital span task and word-pair task, respectively. The portable neurofeedback system had advantages of a tiny size and long-term recording and demonstrated trainability of alpha rhythm in terms of significant increase of power and duration of 8-12 Hz. Moreover, accuracies of the backward digital span task and word-pair task showed significant enhancement in the Alpha group after training compared to the control group. Our tiny portable device demonstrated success trainability of alpha rhythm and enhanced two kinds of memories. The present study suggest that the portable neurofeedback system provides an alternative intervention for memory enhancement.

21 CFR 866.5425 - Alpha-2-glycoproteins immunological test system.

Code of Federal Regulations, 2010 CFR

2010-04-01

... the alpha-2-glycoproteins (a group of plasma proteins found in the alpha-2 group when subjected to... some cancers and genetically inherited deficiencies of these plasma proteins. (b) Classification. Class...

21 CFR 866.5425 - Alpha-2-glycoproteins immunological test system.

Code of Federal Regulations, 2012 CFR

2012-04-01

... the alpha-2-glycoproteins (a group of plasma proteins found in the alpha-2 group when subjected to... some cancers and genetically inherited deficiencies of these plasma proteins. (b) Classification. Class...

21 CFR 866.5425 - Alpha-2-glycoproteins immunological test system.

Code of Federal Regulations, 2011 CFR

2011-04-01

... the alpha-2-glycoproteins (a group of plasma proteins found in the alpha-2 group when subjected to... some cancers and genetically inherited deficiencies of these plasma proteins. (b) Classification. Class...

21 CFR 866.5425 - Alpha-2-glycoproteins immunological test system.

Code of Federal Regulations, 2013 CFR

2013-04-01

... the alpha-2-glycoproteins (a group of plasma proteins found in the alpha-2 group when subjected to... some cancers and genetically inherited deficiencies of these plasma proteins. (b) Classification. Class...

21 CFR 866.5425 - Alpha-2-glycoproteins immunological test system.

Code of Federal Regulations, 2014 CFR

2014-04-01

... the alpha-2-glycoproteins (a group of plasma proteins found in the alpha-2 group when subjected to... some cancers and genetically inherited deficiencies of these plasma proteins. (b) Classification. Class...

{alpha}+{alpha} scattering reexamined in the context of the Sao Paulo potential

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chamon, L. C.; Gasques, L. R.; Carlson, B. V.

2011-03-15

We have analyzed a large set of {alpha}+{alpha} elastic scattering data for bombarding energies ranging from 0.6 to 29.5 MeV. Because of the complete lack of open reaction channels, the optical interaction at these energies must have a vanishing imaginary part. Thus, this system is particularly important because the corresponding elastic scattering cross sections are very sensitive to the real part of the interaction. The data were analyzed in the context of the velocity-dependent Sao Paulo potential, which is a successful theoretical model for the description of heavy-ion reactions from sub-barrier to intermediate energies. We have verified that, even inmore » this low-energy region, the velocity dependence of the model is quite important for describing the data of the {alpha}+{alpha} system.« less

Alpha models for rotating Navier-Stokes equations in geophysics with nonlinear dispersive regularization

NASA Astrophysics Data System (ADS)

Kim, Bong-Sik

Three dimensional (3D) Navier-Stokes-alpha equations are considered for uniformly rotating geophysical fluid flows (large Coriolis parameter f = 2O). The Navier-Stokes-alpha equations are a nonlinear dispersive regularization of usual Navier-Stokes equations obtained by Lagrangian averaging. The focus is on the existence and global regularity of solutions of the 3D rotating Navier-Stokes-alpha equations and the uniform convergence of these solutions to those of the original 3D rotating Navier-Stokes equations for large Coriolis parameters f as alpha → 0. Methods are based on fast singular oscillating limits and results are obtained for periodic boundary conditions for all domain aspect ratios, including the case of three wave resonances which yields nonlinear "2½-dimensional" limit resonant equations for f → 0. The existence and global regularity of solutions of limit resonant equations is established, uniformly in alpha. Bootstrapping from global regularity of the limit equations, the existence of a regular solution of the full 3D rotating Navier-Stokes-alpha equations for large f for an infinite time is established. Then, the uniform convergence of a regular solution of the 3D rotating Navier-Stokes-alpha equations (alpha ≠ 0) to the one of the original 3D rotating NavierStokes equations (alpha = 0) for f large but fixed as alpha → 0 follows; this implies "shadowing" of trajectories of the limit dynamical systems by those of the perturbed alpha-dynamical systems. All the estimates are uniform in alpha, in contrast with previous estimates in the literature which blow up as alpha → 0. Finally, the existence of global attractors as well as exponential attractors is established for large f and the estimates are uniform in alpha.

Activated release of membrane-anchored TGF-alpha in the absence of cytosol

PubMed Central

1993-01-01

The ectodomain of proTGF-alpha, a membrane-anchored growth factor, is converted into soluble TGF-alpha by a regulated cellular proteolytic system that recognizes proTGF-alpha via the C-terminal valine of its cytoplasmic tail. In order to define the biochemical components involved in proTGF-alpha cleavage, we have used cells permeabilized with streptolysin O (SLO) that have been extensively washed to remove cytosol. PMA, acting through a Ca(2+)-independent protein kinase C, activates cleavage as efficiently in permeabilized cells as it does in intact cells. ProTGF-alpha cleavage is also stimulated by GTP gamma S through a mechanism whose pharmacological properties suggest the involvement of a heterotrimeric G protein acting upstream of the PMA- sensitive Ca(2+)-independent protein kinase C. Activated proTGF-alpha cleavage is dependent on ATP hydrolysis, appears not to require vesicular traffic, and acts specifically on proTGF-alpha that has reached the cell surface. These results indicate that proTGF-alpha is cleaved from the cell surface by a regulated system whose signaling, recognition, and proteolytic components are retained in cells devoid of cytosol. PMID:8314849

Functional Expression of Two Neuronal Nicotinic Acetylcholine Receptors from cDNA Clones Identifies a Gene Family

NASA Astrophysics Data System (ADS)

Boulter, Jim; Connolly, John; Deneris, Evan; Goldman, Dan; Heinemann, Steven; Patrick, Jim

1987-11-01

A family of genes coding for proteins homologous to the α subunit of the muscle nicotinic acetylcholine receptor has been identified in the rat genome. These genes are transcribed in the central and peripheral nervous systems in areas known to contain functional nicotinic receptors. In this paper, we demonstrate that three of these genes, which we call alpha3, alpha4, and beta2, encode proteins that form functional nicotinic acetylcholine receptors when expressed in Xenopus oocytes. Oocytes expressing either alpha3 or alpha4 protein in combination with the beta2 protein produced a strong response to acetylcholine. Oocytes expressing only the alpha4 protein gave a weak response to acetylcholine. These receptors are activated by acetylcholine and nicotine and are blocked by Bungarus toxin 3.1. They are not blocked by α -bungarotoxin, which blocks the muscle nicotinic acetylcholine receptor. Thus, the receptors formed by the alpha3, alpha4, and beta2 subunits are pharmacologically similar to the ganglionic-type neuronal nicotinic acetylcholine receptor. These results indicate that the alpha3, alpha4, and beta2 genes encode functional nicotinic acetylcholine receptor subunits that are expressed in the brain and peripheral nervous system.

Alpha-1 adrenoceptor hyperresponsiveness in three neuropathic pain states: complex regional pain syndrome 1, diabetic peripheral neuropathic pain and central pain states following spinal cord injury.

PubMed

Teasell, Robert W; Arnold, J Malcolm O

2004-01-01

The pathophysiology of the pain associated with complex regional pain syndrome, spinal cord injury and diabetic peripheral neuropathy is not known. The pain of complex regional pain syndrome has often been attributed to abnormal sympathetic nervous system activity based on the presence of vasomotor instability and a frequently reported positive response, albeit a temporary response, to sympathetic blockade. In contrast, the pain below the level of spinal cord injury and diabetic peripheral neuropathy are generally seen as deafferentation phenomena. Each of these pain states has been associated with abnormal sympathetic nervous system function and increased peripheral alpha-1 adrenoceptor activity. This increased responsiveness may be a consequence of alpha-1 adrenoceptor postsynaptic hypersensitivity, or alpha-2 adrenoceptor presynaptic dysfunction with diminished noradrenaline reuptake, increased concentrations of noradrenaline in the synaptic cleft and increased stimulation of otherwise normal alpha-1 adrenoceptors. Plausible mechanisms based on animal research by which alpha-1 adrenoceptor hyperresponsiveness can lead to chronic neuropathic-like pain have been reported. This raises the intriguing possibility that sympathetic nervous system dysfunction may be an important factor in the generation of pain in many neuropathic pain states. Although results to date have been mixed, there may be a greater role for new drugs which target peripheral alpha-2 adrenoceptors (agonists) or alpha-1 adrenoceptors (antagonists).

Generation of ER{alpha}-floxed and knockout mice using the Cre/LoxP system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Antonson, P., E-mail: per.antonson@ki.se; Omoto, Y.; Humire, P.

2012-08-10

Highlights: Black-Right-Pointing-Pointer ER{alpha} floxed and knockout mice were generated. Black-Right-Pointing-Pointer Disruption of the ER{alpha} gene results in sterility in both male and female mice. Black-Right-Pointing-Pointer ER{alpha}{sup -/-} mice have ovaries with hemorrhagic follicles and hypoplastic uterus. Black-Right-Pointing-Pointer Female ER{alpha}{sup -/-} mice develop obesity. -- Abstract: Estrogen receptor alpha (ER{alpha}) is a nuclear receptor that regulates a range of physiological processes in response to estrogens. In order to study its biological role, we generated a floxed ER{alpha} mouse line that can be used to knock out ER{alpha} in selected tissues by using the Cre/LoxP system. In this study, we established amore » new ER{alpha} knockout mouse line by crossing the floxed ER{alpha} mice with Cre deleter mice. Here we show that genetic disruption of the ER{alpha} gene in all tissues results in sterility in both male and female mice. Histological examination of uterus and ovaries revealed a dramatically atrophic uterus and hemorrhagic cysts in the ovary. These results suggest that infertility in female mice is the result of functional defects of the reproductive tract. Moreover, female knockout mice are hyperglycemic, develop obesity and at the age of 4 months the body weight of these mice was more than 20% higher compared to wild type littermates and this difference increased over time. Our results demonstrate that ER{alpha} is necessary for reproductive tract development and has important functions as a regulator of metabolism in females.« less

3D cultured immortalized human hepatocytes useful to develop drugs for blood-borne HCV

DOE Office of Scientific and Technical Information (OSTI.GOV)

Aly, Hussein Hassan; Shimotohno, Kunitada; Hijikata, Makoto

2009-02-06

Due to the high polymorphism of natural hepatitis C virus (HCV) variants, existing recombinant HCV replication models have failed to be effective in developing effective anti-HCV agents. In the current study, we describe an in vitro system that supports the infection and replication of natural HCV from patient blood using an immortalized primary human hepatocyte cell line cultured in a three-dimensional (3D) culture system. Comparison of the gene expression profile of cells cultured in the 3D system to those cultured in the existing 2D system demonstrated an up-regulation of several genes activated by peroxisome proliferator-activated receptor alpha (PPAR{alpha}) signaling. Furthermore,more » using PPAR{alpha} agonists and antagonists, we also analyzed the effect of PPAR{alpha} signaling on the modulation of HCV replication using this system. The 3D in vitro system described in this study provides significant insight into the search for novel anti-HCV strategies that are specific to various strains of HCV.« less

Progressive accumulation of ubiquitin and disappearance of alpha-synuclein epitope in multiple system atrophy-associated glial cytoplasmic inclusions: triple fluorescence study combined with Gallyas-Braak method.

PubMed

Sakamoto, Masaki; Uchihara, Toshiki; Nakamura, Ayako; Mizutani, Toshio; Mizusawa, Hidehiro

2005-10-01

Alpha-synuclein (alphaS) and ubiquitin (Ub) are shared constituents of glial cytoplasmic inclusions (GCIs) and Lewy bodies (LBs), both composed of fibrillary structures. Staining profiles of GCIs were investigated with triple immunofluorescence involving immunostaining for alphaS and Ub, both amplified with catalyzed reporter deposition, and a fluorochrome, thiazin red (TR) that has an affinity to fibrillary structures. After observation for the triple-fluorescent images, the sections were subsequently stained with the Gallyas-Braak method. Sections of putamen, cerebellar white matter and motor cortex from patients suffering from multiple system atrophy (MSA) with varying duration of the disease (4-15 years) were quantified for these staining profiles of Gallyas-positive GCIs. Although most of GCIs were positive for Ub and variably positive for alphaS, they were consistently negative for TR. The result was opposite in LBs in Lewy body disease with variable affinity to TR, suggesting that the construction of GCIs is different from that of LBs. These four staining features (alphaS, Ub, TR and Gallyas) alone failed to exhibit apparent correlation with disease duration, lesion site or severity of degeneration as reported previously. The fraction of alphaS-negative and Ub-positive GCIs, however, linearly increased along the disease progression, while that of alphaS-positive and Ub-negative GCIs decreased in contrast. This reciprocal change suggests that alphaS immunoreactivity in GCIs is being replaced by Ub immunoreactivity during the disease progression, which resulted in the ultimate predominance of alphaS-negative and Ub-positive GCIs in the most advanced case. Interestingly, this predominance of alphaS-negative and Ub-positive GCIs was a feature of motor cortex, where degeneration usually remains mild in spite of robust appearance of Gallyas-positive GCIs. Another fraction, alphaS-positive and Ub-positive GCIs were frequent in cerebellar white matter, suggesting that GCI evolution is heterogeneous and dependent also on area examined. Progressive accumulation of Ub with concomitant disappearance of alphaS epitope and their colocalization, partly shared with LBs, may represent a process of GCI formation, possibly linked to an aspect of degeneration in MSA.

alpha2-chimaerin, a Cdc42/Rac1 regulator, is selectively expressed in the rat embryonic nervous system and is involved in neuritogenesis in N1E-115 neuroblastoma cells.

PubMed

Hall, C; Michael, G J; Cann, N; Ferrari, G; Teo, M; Jacobs, T; Monfries, C; Lim, L

2001-07-15

Neuronal differentiation involves Rac and Cdc42 GTPases. alpha-Chimaerin, a Rac/Cdc42 regulator, occurs as alpha1- and alternatively spliced Src homology 2 (SH2) domain-containing alpha2-isoforms. alpha2-chimaerin mRNA was highly expressed in the rat embryonic nervous system, especially in early postmitotic neurons. alpha1-chimaerin mRNA was undetectable before embryonic day 16.5. Adult alpha2-chimaerin mRNA was restricted to neurons within specific brain regions, with highest expression in the entorhinal cortex. alpha2-chimaerin protein localized to neuronal perikarya, dendrites, and axons. The overall pattern of alpha2-chimaerin mRNA expression resembles that of cyclin-dependent kinase regulator p35 (CDK5/p35) which participates in neuronal differentiation and with which chimaerin interacts. To determine whether alpha2-chimaerin may have a role in neuronal differentiation and the relevance of the SH2 domain, the morphological effects of both chimaerin isoforms were investigated in N1E-115 neuroblastoma cells. When plated on poly-lysine, transient alpha2-chimaerin but not alpha1-chimaerin transfectants formed neurites. Permanent alpha2-chimaerin transfectants generated neurites whether or not they were stimulated by serum starvation, and many cells were enlarged. Permanent alpha1-chimaerin transfectants displayed numerous microspikes and contained F-actin clusters, a Cdc42-phenotype, but generated few neurites. In neuroblastoma cells, alpha2-chimaerin was predominantly soluble with some being membrane-associated, whereas alpha1-chimaerin was absent from the cytosol, being membrane- and cytoskeleton-associated, paralleling their subcellular distribution in brain. Transient transfection with alpha2-chimaerin mutated in the SH2 domain (N94H) generated an alpha1-chimaerin-like phenotype, protein partitioned in the particulate fraction, and in NGF-stimulated pheochromocytoma cell line 12 (PC12) cells, neurite formation was inhibited. These results indicate a role for alpha2-chimaerin in morphological differentiation for which its SH2 domain is vital.

Mesospheric Simulations with the NOGAPS-ALPHA model: Applications to the Summer Polar Mesosphere and AIM data

NASA Astrophysics Data System (ADS)

Siskind, D. E.; Eckermann, S. D.; McCormack, J. P.; Hoppel, K. W.; Russell, J. M.; Bailey, S.; Hervig, M.; Rusch, D.

2007-12-01

The Navy Operational Global Atmospheric Prediction System (NOGAPS), the Department of Defense's global numerical weather prediction (NWP) system, consists of two main components: the Naval Research Laboratory (NRL) Atmospheric Variational Data Assimilation System (NAVDAS) and a global spectral general circulation model (GCM) for forecasting. NRL researchers are currently developing an Advanced-Level Physics High-Altitude (ALPHA) NOGAPS prototype that extends the currently operational 1 hPa upper boundary of NOGAPS through the mesosphere and lower thermosphere (MLT) to ~110 km. We report results of preliminary experiments with this NOGAPS-ALPHA system during May-June 2007, focused on the northern hemisphere (NH) summer mesosphere observed from the Aeronomy of Ice in the Mesosphere (AIM) satellite. These AIM-period NOGAPS-ALPHA experiments have two main goals: to provide global modeling support for AIM science and to allow objective validation of these new NOGAPS-ALPHA MLT fields using independent observations from AIM. We report results of runs which assimilate temperature and water vapor data from the SABER and MLS instruments up to ~0.01 hPa. We investigate the development of the cold NH summer mesopause in NOGAPS-ALPHA and its sensitivity to parameterized nonorographic gravity wave drag (GWD) and radiative heating/cooling by comparing with temperatures and water vapor measured by AIM's SOFIE instrument. We can also compare the variability in the NOGAPS-ALPHA temperature and water vapor fields with mesospheric cloud occurrence statistics measured by CIPS on AIM.

A Strategic Roadmap to Centauri

NASA Technical Reports Server (NTRS)

Johnson, Les; Harris, David; Trausch, Ann; Matloff, Gregory L.; Taylor, Travis; Cutting, Kathleen

2005-01-01

This paper discusses the connectivity between in-space propulsion and in-space fabrication/repair and is based upon a workshop presentation by Les Johnson, manager of the In-Space Propulsion (ISP) Technology Project at NASA's Marshall Space Flight Center (MSFC) in Huntsville, Ala.. Technologies under study by ISP include aerocapture, advanced solar-electric propulsion, solar-thermal propulsion, advanced chemical propulsion, tethers and solar-photon sails. These propulsion systems are all approaching technology readiness levels (TRLs) at which they can be considered for application in space-science and exploration missions. Historically, human frontiers have expanded as people have learned to live off the land in new environments and to exploit local resorces. With this expansion, frontier settlements have required development of transportation improvements to carry tools and manufactured products to and from the frontier. It is demonstrated how ISP technologies will assist in the development of the solar-system frontier. In-space fabrication and repair will both require and assist the development of ISP propulsion systems, whether humans choose to settle planetary surfaces or to exploit resources of small Solar System bodies. As was true for successful terrestrial pioneers, in-space settlement and exploitation will require sophisticated surveys of inner and outer Solar System objects. ISP technologies will contribute to the success of these surveys, as well as to the efforts to retrieve Solar System resources. In a similar fashion, the utility of ISP products will be greatly enhanced by the technologies of in-space repair and fabrication. As in-space propulsion, fabrication and repair develop, human civilization may expand well beyond the Earth. In the future, small human communities (preceded by robotic explorers) may utilize these techniques to set sail f or the nearest stars.

A Strategic Roadmap to Centauri

NASA Astrophysics Data System (ADS)

Johnson, L.; Harris, D.; Trausch, A.; Matloff, G. L.; Taylor, T.; Cutting, K.

This paper discusses the connectivity between in-space propulsion and in-space fabrication/repair and is based upon a workshop presentation by Les Johnson, manager of the In-Space Propulsion (ISP) Technology Project at NASA's Marshall Space Flight Center (MSFC) in Huntsville, Alabama. Technologies under study by ISP include aerocapture, advanced solar- electric propulsion, solar-thermal propulsion, advanced chemical propulsion, tethers and solar-photon sails. These propulsion systems are all approaching technology readiness levels (TRLs) at which they can be considered for application in space- science and exploration missions. Historically, human frontiers have expanded as people have learned to “live-off-the-land” in new environments and to exploit local resources. With this expansion, frontier settlements have required development of transportation improvements to carry tools and manufactured products to and from the frontier. It is demonstrated how ISP technologies will assist in the development of the solar-system frontier. In-space fabrication and repair will both require and assist the development of ISP propulsion systems, whether humans choose to settle planetary surfaces or to exploit resources of small Solar System bodies. As was true for successful terrestrial pioneers, in-space settlement and exploitation will require sophisticated surveys of inner and outer Solar System objects. ISP technologies will contribute to the success of these surveys, as well as to the efforts to retrieve Solar System resources. In a similar fashion, the utility of ISP products will be greatly enhanced by the technologies of in-space repair and fabrication. As in-space propulsion, fabrication and repair develop, human civilization may expand well beyond the Earth. In the future, small human communities (preceded by robotic explorers) may utilize these techniques to set sail for the nearest stars.

Development of a personal dosimetry system based on optically stimulated luminescence of alpha-Al2O3:C for mixed radiation fields.

PubMed

Lee, S Y; Lee, K J

2001-04-01

To develop a personal optically stimulated luminescence (OSL) dosimetry system for mixed radiation fields using alpha-Al2O3:C, a discriminating badge filter system was designed by taking advantage of its optically stimulable properties and energy dependencies. This was done by designing a multi-element badge system for powder layered alpha-Al2O3:C material and an optical reader system based on high-intensity blue light-emitting diode (LED). The design of the multielement OSL dosimeter badge system developed allows the measurement of a personal dose equivalent value Hp(d) in mixed radiation fields of beta and gamma. Dosimetric properties of the personal OSL dosimeter badge system investigated here were the dose response, energy response and multi-readability. Based on the computational simulations and experiments of the proposed dosimeter design, it was demonstrated that a multi-element dosimeter system with an OSL technology based on alpha-Al2O3:C is suitable to obtain personal dose equivalent information in mixed radiation fields.

Enhanced homologous recombination is induced by alpha-particle radiation in somatic cells of Arabidopsis thaliana

NASA Astrophysics Data System (ADS)

Bian, Po; Liu, Ping; Wu, Yuejin

Almost 9 percent of cosmic rays which strike the earth's atmosphere are alpha particles. As one of the ionizing radiations (IR), its biological effects have been widely studied. However, the plant genomic instability induced by alpha-particle radiation was not largely known. In this research, the Arabidopsis thaliana transgenic for GUS recombination substrate was used to evaluate the genomic instability induced by alpha-particle radiation (3.3MeV). The pronounced effects of systemic exposure to alpha-particle radiation on the somatic homologous recombination frequency (HRF) were found at different doses. The 10Gy dose of radiation induced the maximal HRF which was 1.9-fold higher than the control. The local radiation of alpha-particle (10Gy) on root also resulted in a 2.5-fold increase of somatic HRF in non-radiated aerial plant, indicating that the signal(s) of genomic instability was transferred to non-radiated parts and initiated their genomic instability. Concurrent treatment of seedlings of Arabidopsis thaliana with alpha-particle and DMSO(ROS scavenger) both in systemic and local radiation signifi- cantly suppressed the somatic HR, indicating that the free radicals produced by alpha-particle radiation took part in the production of signal of genomic instability rather than the signal transfer. Key words: alpha-particle radiation, somatic homologous recombination, genomic instability

[Locally administered lentivirus-mediated siRNA inhibits wear debris-induced inflammation].

PubMed

Peng, Xiao-chun; Zhang, Xian-long; Tao, Kun; Cheng, Tao; Zhu, Jun-feng; Zeng, Bing-fang

2009-03-01

To determine the safety and efficacy of local administration of lentivirus-mediated small interfering RNA (siRNA) targeting tumor necrosis factor-alpha (TNF-alpha) in murine air pouch model. From May 2007 to April 2008 a siRNA targeting TNF-alpha and a missense siRNA were designed, and recombine lentivirus which coexpressed the green fluorescent protein (GFP) as a marker gene was constructed. Air pouches were established and stimulated by Ti-6Al-4V particles. Pouches were divided into 3 groups randomly. Lentivirus-mediated siRNA targeting TNF-alpha (TNF-alpha group) or lentivirus-mediated missense siRNA (MS group), or virus-free saline (control group) were injected into pouches respectively. Pouch membrane, peripheral blood, heart, liver, spleen, kidney, lung and brain were harvested at 28 d after transfection, and assayed for markers of inflammation using histological, molecular, immunological techniques and Xenogen in vivo imaging system (IVIS) 50 vivo bioluminescent assay system. Xenogen IVIS 50 vivo image revealed strong expression of GFP localized in pouch areas and no expression in other parts of mice both in TNF-alpha group and MS group at 4 weeks after transfection, while no expression of GFP was found in control group. By RT-PCR and ELISA, the mRNA and protein levels of TNF-alpha in TNF-alpha group decreased by 81.6% and 82.6% respectively compared to control group (P < 0.01), and decreased by 78.9% and 84.0% respectively compared to MS group (P < 0.01), whereas TNF-alpha level in peripheral blood, heart, liver, spleen, kidney, lung and brain remained invariant (P > 0.05). Less inflammatory responses (thinner pouch membrane and decreased cellular infiltration) were observed in TNF-alpha group. Efficient local delivery of lentivirus-mediated siRNA targeting TNF-alpha into modified murine air pouch can inhibit debris-induced inflammation effectively, with no systemic adverse effects.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Feng,L.; Gell, D.; Zhou, S.

Hemoglobin A (HbA), the oxygen delivery system in humans, comprises two alpha and two beta subunits. Free alpha-hemoglobin (alphaHb) is unstable, and its precipitation contributes to the pathophysiology of beta thalassemia. In erythrocytes, the alpha-hemoglobin stabilizing protein (AHSP) binds alphaHb and inhibits its precipitation. The crystal structure of AHSP bound to Fe(II)-alphaHb reveals that AHSP specifically recognizes the G and H helices of alphaHb through a hydrophobic interface that largely recapitulates the alpha1-beta1 interface of hemoglobin. The AHSP-alphaHb interactions are extensive but suboptimal, explaining why beta-hemoglobin can competitively displace AHSP to form HbA. Remarkably, the Fe(II)-heme group in AHSP boundmore » alphaHb is coordinated by the distal but not the proximal histidine. Importantly, binding to AHSP facilitates the conversion of oxy-alphaHb to a deoxygenated, oxidized [Fe(III)], nonreactive form in which all six coordinate positions are occupied. These observations reveal the molecular mechanisms by which AHSP stabilizes free alphaHb.« less

Overproduction of alpha chains provides a proton-insensitive component to the bluefish hemoglobin system.

PubMed

Bonaventura, Celia; Godette, Gerald; Stevens, Robert; Brenowitz, Michael; Henkens, Robert

2005-12-09

Expression of alpha and beta chains and their post-translational assembly into alpha(2)beta(2) tetramers is fundamental to the formation and function of most vertebrate hemoglobins. There is a strong evolutionary bias that favors expression of equal amounts of the two types of chains, because cooperativity, pH sensitivity, and anionic control of function occurs only for the alpha(2)beta(2) tetramers. Remarkably, an over-production of alpha chains, as in the pathological condition known as beta thalassemia in humans, is adaptive rather than pathological in the bluefish hemoglobin system. The thalassemia of the bluefish is a novel means of providing for oxygen uptake and delivery when low pH conditions incapacitate the highly pH-sensitive Root effect hemoglobins of the fish. Although fish often have pH-insensitive along with highly pH-sensitive hemoglobins, having pH-insensitive alpha chain monomers in circulation is an unusual structural variation. The role of bluefish alpha chains in oxygen transport is enabled by their remarkably lower oxygen affinity relative to human alpha chains. This is the first reported case of a thalassemic condition that is maintained in a species as an adaptive advantage.

Taxonomy of the Streptomyces strain ZG0656 that produces acarviostatin alpha-amylase inhibitors and analysis of their effects on blood glucose levels in mammalian systems.

PubMed

Geng, P; Bai, G; Shi, Q; Zhang, L; Gao, Z; Zhang, Q

2009-02-01

To clarify the taxonomic status of strain ZG0656 and analyse the effects of its acarviostatin products on blood glucose levels in mammalian systems. Our program to screen for new alpha-amylase inhibitors led to the isolation of strain ZG0656. The polyphasic taxonomic study revealed that strain ZG0656 represents a novel variation of Streptomyces coelicoflavus, for which we propose the name S. coelicoflavus var. nankaiensis. Four chemically distinct alpha-amylase inhibitors, acarviostatins I03, II03, III03 and IV03, were isolated from strain ZG0656. Acarviostatins III03 and IV03 are both novel oligomers. All four acarviostatins are mixed noncompetitive porcine pancreas alpha-amylase inhibitors. Acarviostatin III03 is the most potent alpha-amylase inhibitor known to date. Moreover, in the in vitro and in vivo experiments, acarviostatins III03 showed significant inhibition of starch hydrolysis and glucose transfer to blood. Strain ZG0656 is a novel variation of S. coelicoflavus, whose products are novel effective alpha-amylase inhibitors. Among the products, acarviostatins III03 could significantly depress blood glucose levels in mammalian systems and be developed towards a possible therapeutic agent for diabetes. Acarviostatin III03 is the most potent alpha-amylase inhibitor known to date. The oligomer will benefit the research on the relationship between alpha-amylase and various inhibitors and will offer more choices in diabetes treatments.

[Influences of R2O-Al2O3-B2O3-SiO2 system glass and superfine alpha-Al2O3 on the sintering and phase transition of hydroxyapatite ceramics].

PubMed

Wang, Zhiqiang; Chen, Xiaoxu; Cai, Yingji; Lü, Bingling

2003-06-01

The effects of R2O-Al2O3-B2O3-SiO2 system glass and superfine alpha-Al2O3 on the sintering and phase transition of hydroxyapatite (HAP) ceramics were assessed. The results showed that alpha-Al2O3 impeded the sintering of HAP and raised the sintering temperature. When glass and alpha-Al2O3 were used together to reinforce HAP ceramics, better results could be obtained; the bending strength of multiphase HAP ceramics approached 106 MPa when 10% (wt) alpha-Al2O3 and 20%(wt) glass were used and sintered at 1200 for 1 h.

A high-level prokaryotic expression system: synthesis of human interleukin 1 alpha and its receptor antagonist.

PubMed

Birikh, K R; Lebedenko, E N; Boni, I V; Berlin, Y A

1995-10-27

Synthetic intronless genes, coding for human interleukin 1 alpha (IL 1 alpha) and interleukin 1 receptor antagonist (IL1ra), have been expressed efficiently in a specially designed prokaryotic vector, pGMCE (a pGEM1 derivative), where the target gene forms the second part of a two-cistron system. The first part of the system is a translation enhancer-containing mini-cistron, whose termination codon overlaps the start codon of the target gene. In the case of the IL1 alpha gene, the high expression level is largely due to the direct efficient translation initiation at the second cistron, whereas with the IL1ra gene in the same system, the proximal translation initiation region (TIR) provides a high level of coupled expression of the target gene. Thus, pGMCE is a potentially versatile vector for direct prokaryotic expression.

RR LYRAE ATMOSPHERICS: WRINKLES OLD AND NEW. A PREVIEW

DOE Office of Scientific and Technical Information (OSTI.GOV)

Preston, George W., E-mail: gwp@obs.carnegiescience.ed

I report some results of an echelle spectroscopic survey of RR Lyrae stars begun in 2006 that I presented in my Henry Norris Lecture of 2010 January 4. Topics include (1) atmospheric velocity gradients, (2) phase-dependent envelope turbulence as it relates to Peterson's discoveries of axial rotation on the horizontal branch and to Stothers' explanation of the Blazhko effect, (3) the three apparitions of hydrogen emission during a pulsation cycle, (4) the occurrence of He I lines in emission and absorption, (5) detection of He II emission and metallic line doubling in Blazhko stars, and finally (6) speculation about whatmore » helium observations of RR Lyrae stars in omega Centauri might tell us about the putative helium populations and the horizontal branch of that strange globular cluster.« less

P alpha-chiral phosphorothioate analogues of bis(5'-adenosyl)tetraphosphate (Ap4A); their enzymatic synthesis and degradation.

PubMed Central

Lazewska, D; Guranowski, A

1990-01-01

Synthesis of Sp and Rp diastereomers of Ap4A alpha S has been characterized in two enzymatic systems, the lysyl-tRNA synthetase from Escherichia coli and the Ap4A alpha, beta-phosphorylase from Saccharomyces cerevisiae. The synthetase was able to use both (Sp)ATP alpha S and (Rp)ATP alpha S as acceptors of adenylate thus yielding corresponding monothioanalogues of Ap4A,(Sp) Ap4A alpha S and (Rp)Ap4A alpha S. No dithiophosphate analogue was formed. Relative synthetase velocities of the formation of Ap4A,(Sp) Ap4A alpha S and (Rp)Ap4A alpha S were 1:0.38:0.15, and the computed Km values for (Sp)ATP alpha S and (Rp)ATP alpha S were 0.48 and 1.34 mM, respectively. The yeast Ap4A phosphorylase synthesized (Sp)Ap4A alpha S and (Rp)Ap4A alpha S using adenosine 5'-phosphosulfate (APS) as source of adenylate. The adenylate was accepted by corresponding thioanalogues of ATP. In that system, relative velocities of Ap4A, (Sp)Ap4A alpha S and (Rp)Ap4A alpha S formation were 1:0.15:0.60. The two isomeric phosphorothioate analogues of Ap4A were tested as substrates for the following specific Ap4A-degrading enzymes: (asymmetrical) Ap4A hydrolase (EC 3.6.1.17) from yellow lupin (Lupinus luteus) seeds hydrolyzed each of the analogues to AMP and the corresponding isomer of ATP alpha S; (symmetrical) Ap4A hydrolase (EC 3.6.1.41) from E. coli produced ADP and the corresponding diastereomer of ADP alpha S; and Ap4A phosphorylase (EC 2.7.7.53) from S. cerevisiae cleaved the Rp isomer only at the unmodified end yielding ADP and (Rp)ATP alpha S whereas the Sp isomer was degraded non-specifically yielding a mixture of ADP, (Sp)ADP alpha S, ATP and (Sp)ATP alpha S. For all the Ap4A-degrading enzymes, the Rp isomer of Ap4A alpha S appeared to be a better substrate than its Sp counterpart; stereoselectivity of the three enzymes for the Ap4A alpha S diastereomers is 51, 6 and 2.5, respectively. Basic kinetic parameters of the degradation reactions are presented and structural requirements of the Ap4A-metabolizing enzymes with respect to the potential substrates modified at the Ap4A-P alpha are discussed. PMID:2172926

Evaluation of GABAergic neuroactive steroid 3alpha-hydroxy-5alpha-pregnane-20-one as a neurobiological substrate for the anti-anxiety effect of ethanol in rats.

PubMed

Hirani, Khemraj; Sharma, Ajay N; Jain, Nishant S; Ugale, Rajesh R; Chopde, Chandrabhan T

2005-07-01

Acute systemic ethanol administration is known to elevate plasma and cerebral levels of neuroactive steroid 3alpha-hydroxy-5alpha-pregnane-20-one (3alpha, 5alpha-THP; allopregnanolone) to a concentration sufficient to potentiate GABA(A) receptors. We have earlier demonstrated that 3alpha, 5alpha-THP mediates the antidepressant-like effect of ethanol in Porsolt forced swim test. The aim of the present study is to explain the relationship between endogenous GABAergic neurosteroids and anxiolytic effect of ethanol in Sprague-Dawley rats. The mediation of 3alpha, 5alpha-THP in the anti-anxiety effect of ethanol was assessed by pharmacological interactions of ethanol with various endogenous neurosteroidal modulators and using simulated physiological conditions of altered neurosteroid content in elevated plus maze (EPM) test. Pretreatment of 3alpha, 5alpha-THP (0.5-2.5 mug/rat, i.c.v.) or neurosteroidogenic agents such as 3alpha, 5alpha-THP precursor progesterone (5 or 10 mg/kg, i.p.), 11-beta hydroxylase inhibitor metyrapone (50 or 100 mg/kg, i.p.) or the GABA(A) receptor agonist muscimol (25 ng/rat, i.c.v.) significantly potentiated the anti-anxiety effect of ethanol (1 g/kg, i.p.). On the other hand, the GABAergic antagonistic neurosteroid dehydroepiandrosterone sulphate (DHEAS) (1 mg/kg, i.p.), the GABA(A) receptor blocker bicuculline (1 mg/kg, i.p.), the 5alpha-reductase inhibitor finasteride (50 x 2 mg/kg, s.c.) or the mitochondrial diazepam binding inhibitory receptor antagonist PK11195 (1 mg/kg, i.p.) reduced ethanol-induced preference of time spent and number of entries into open arms. Anti-anxiety effect of ethanol was abolished in adrenalectomized (ADX) rats as compared to sham-operated control. This ADX-induced blockade was restored by prior systemic injection of progesterone, signifying the contribution of peripheral steroidogenesis in ethanol anxiolysis. Socially isolated animals known to exhibit decreased brain 3alpha, 5alpha-THP and GABA(A) receptor functions displayed reduced sensitivity to the effects of ethanol and 3alpha, 5alpha-THP in EPM test. Our results demonstrated the contributory role of neuroactive steroid 3alpha, 5alpha-THP in the anti-anxiety effect of ethanol. It is speculated that ethanol-induced modulation of endogenous GABAergic neurosteroids, especially 3alpha, 5alpha-THP, might be crucial pertinent to the etiology of 'trait' anxiety (tension reduction) and ethanol abuse.

Addition products of alpha-tocopherol with lipid-derived free radicals.

PubMed

Yamauchi, Ryo

2007-01-01

The addition products of alpha-tocopherol with lipid-derived free radicals have been reviewed. Free radical scavenging reactions of alpha-tocopherol take place via the alpha-tocopheroxyl radical as an intermediate. If a suitable free radical is present, an addition product can be formed from the coupling of the free radical with the alpha-tocopheroxyl radical. The addition products of alpha-tocopherol with lipid-peroxyl radicals are 8a-(lipid-dioxy)-alpha-tocopherones, which are hydrolyzed to alpha-tocopherylquinone. On the other hand, the carbon-centered radicals of lipids prefer to react with the phenoxyl radical of alpha-tocopherol to form 6-O-lipid-alpha-tocopherol under anaerobic conditions. The addition products of alpha-tocopherol with peroxyl radicals (epoxylinoleoyl-peroxyl radicals) produced from cholesteryl ester and phosphatidylcholine were detected in the peroxidized human plasma using a high-sensitive HPLC procedure with postcolumn reduction and electrochemical detection. Thus, the formation of these addition products provides us with much information on the antioxidant function of vitamin E in biological systems.

Circulating neuroactive C21- and C19-steroids in young men before and after ejaculation.

PubMed

Stárka, L; Hill, M; Havlíková, H; Kancheva, L; Sobotka, V

2006-01-01

Twelve neuroactive and neuroprotective steroids, androgens and androgen precursors i.e. 3alpha,17beta-dihydroxy-5alpha-androstane, 3alpha-hydroxy-5alpha-androstan-17-one, 3alpha-hydroxy-5beta-androstan-17-one, androst-5-ene-3beta,17beta-diol, 3beta,17alpha-dihydroxy-pregn-5-en-20-one (17alpha-hydroxy-pregnenolone), 3beta-hydroxy-androst-5-en-17-one (dehydroepiandrosterone, DHEA), testosterone, androst-4-ene-3,17-dione (androstenedione), 3alpha-hydroxy-5alpha-pregnan-20-one (allopregnanolone), 3beta-hydroxy-pregn-5-en-20-one (pregnenolone), 7alpha-hydroxy-DHEA, and 7beta-hydroxy-DHEA were measured using the GC-MS system in young men before and after ejaculation provoked by masturbation. The circulating level of 17alpha-hydroxypregnenolone increased significantly, whereas the other circulating steroids were not changed at all. This fact speaks against the hypothesis that a drop in the level of neuroactive steroids, e.g. allopregnanolone may trigger the orgasm-related increase of oxytocin, reported by other authors.

Progesterone's 5 alpha-reduced metabolite, 3 alpha,5 alpha-THP, mediates lateral displacement of hamsters.

PubMed

Frye, Cheryl A; Rhodes, Madeline E

2005-03-15

5 alpha-Pregnan-3 alpha-ol-20-one (3 alpha,5 alpha-THP), progesterone (P4)'s 5 alpha-reduced, 3 alpha-hydroxysteroid oxidoreduced product, facilitates lordosis of rodents in part via agonist-like actions at GABA(A)/benzodiazepine receptor complexes in the ventral tegmental area (VTA). Whether 3 alpha,5 alpha-THP influences another reproductively-relevant behavior, lateral displacement, of hamsters was investigated. Lateral displacement is the movement that female hamsters make with their perineum towards male-like tactile stimulation. This behavior facilitates, and is essential for, successful mating. Hamsters in behavioral estrus had greater lateral displacement responses when endogenous progestin levels were elevated compared to when progestin levels were lower. Administration of P4, a prohormone for 3 alpha,5 alpha-THP, dose-dependently (500 > 200 > 100, 50, or 0 microg) enhanced lateral displacement of ovariectomized hamsters that had been primed with SC estradiol benzoate (5 or 10 microg). Inhibiting P4's metabolism to 3 alpha,5 alpha-THP by co-administering finasteride, a 5 alpha-reductase inhibitor, or indomethacin, a 3 alpha-hydroxysteroid oxidoreductase inhibitor, either systemically or to the VTA, significantly decreased lateral displacement and midbrain progestin levels of naturally receptive or hormone-primed hamsters compared to controls. These data suggest that lateral displacement is progestin-sensitive and requires the formation of 3 alpha,5 alpha-THP in the midbrain VTA.

Systemic production of IFN-alpha by garlic (Allium sativum) in humans.

PubMed

Bhattacharyya, Mau; Girish, G V; Karmohapatra, Soumendra K; Samad, S A; Sinha, Asru K

2007-05-01

The effect of foods on the production of interferon-alpha (IFN-alpha) is currently unknown. Garlic (Allium sativum) used as a folk medicine is reported to stimulate nitric oxide (NO) production. We investigated the systemic increase of NO due to the ingestion of garlic on the plasma IFN-alpha level in normal volunteers. Normal volunteers (10 groups, 10 in each group) ate 2 g fresh garlic, and plasma NO and IFN-alpha levels were determined after 2 and 4 h. The participants were also asked to eat garlic for various periods of time, and plasma NO and IFN-alpha were similarly assayed. Ingestion of 2 g fresh, but not boiled, garlic was found to increase the basal plasma level of NO from 2.7 +/- 0.1 microM to 8.76 +/- 0.21 microM at 2 and 4 h, respectively. The basal plasma IFN-alpha level increased from 9.51 +/- 0.26 nM to 46.3 +/- 1.2 nM in normal volunteers (n = 10) at the same time. The chronic eating of garlic was found to maintain IFN-alpha at high levels for at least 7 days. The exposure of neutrophils to garlic in vivo or in vitro, which also stimulated synthesis of NO in these cells, was found to stimulate IFN-alpha synthesis as measured by the stimulation of IFN-alpha mRNA synthesis. Thus, consumption of garlic resulted in stimulated synthesis of NO and, in turn, IFN-alpha in humans, which could be beneficial in viral or proliferative diseases.

Developmental changes in the role of a pertussis toxin sensitive guanine nucleotide binding protein in the rat cardiac alpha sub 1 -adrenergic system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Han, H.M.

1989-01-01

During development, the cardiac alpha{sub 1}-adrenergic chronotropic response changes from positive in the neonate to negative in the adult. This thesis examined the possibility of a developmental change in coupling of a PT-sensitive G-protein to the alpha{sub 1}-adrenergic receptor. Radioligand binding experiments performed with the iodinated alpha{sub 1}-selective radioligand ({sup 125}I)-I-2-({beta}-(4-hydroxphenyl)ethylaminomethyl)tetralone (({sup 125}I)-IBE 2254) demonstrated that the alpha{sub 1}-adrenergic receptor is coupled to a G-protein in both neonatal and adult rat hearts. However, in the neonate the alpha{sub 1}-adrenergic receptor is coupled to a PT-insensitive G-protein, whereas in the adult the alpha{sub 1}-adrenergic receptor is coupled to both a PT-insensitivemore » and a PT-sensitive G-protein. Consistent with the results from binding experiments, PT did not have any effect on the alpha{sub 1}-mediated positive chronotropic response in the neonate, whereas in the adult the alpha{sub 1}-mediated negative chronotropic response was completely converted to a positive one after PT-treatment. This thesis also examined the possibility of an alteration in coupling of the alpha{sub 1}-adrenergic receptor to its effector under certain circumstances such as high potassium (K{sup +}) depolarization in nerve-muscle (NM) co-cultures, a system which has been previously shown to be a convenient in vitro model to study the mature inhibitory alpha{sub 1}-response.« less

The alpha subunit of the epithelial sodium channel in the mouse: developmental regulation of its expression.

PubMed

Dagenais, A; Kothary, R; Berthiaume, Y

1997-09-01

Sodium reabsorption by the amiloride-sensitive sodium channel of epithelial cells plays a crucial role in the management of ionic composition and fluid volume in the body. In the respiratory system, sodium transport is involved in the clearance of pulmonary edema and of liquid secreted during fetal life at birth. We have cloned a partial cDNA of the alpha subunit of the mouse amiloride-sensitive sodium channel (alpha mENaC). In the region of comparison, the mouse alpha subunit shows 92% identity at the DNA level and 95% identity at the amino acid level with the rat sequence. The kidneys, lungs, and distal colon are major sites of expression of a 3.5-kb alpha mENaC mRNA. During mouse development, alpha mENaC transcripts appear late during gestation (d 17.5) and are expressed continuously thereafter. In the distal colon, a short 1.2-kb mRNA deleted of the 5' part of the transcript is detected during gestation and is replaced gradually by the mature 3.5-kb transcript after birth. Alpha mENaC and alpha1 Na+-K+-ATPase mRNAs have an expression profile that is modulated similarly during development for a given tissue. The expression of alpha mENaC transcripts increases transiently in the lungs at birth (2.5-fold), as for alpha1 Na+-K+-ATPase mRNAs (1.5-fold), suggesting that the expression of several components of the sodium transport system is modulated in the lungs at that time. In the kidney, there is no significant increase of alpha mENaC and alpha1 Na+-K+-ATPase mRNAs in newborns.

The Dust-to-Gas Ratio in the Damped Ly alpha Clouds Towards the Gravitationally Lensed QSO 0957+561

NASA Technical Reports Server (NTRS)

Zuo, Lin; Beaver, E. A.; Burbidge, E. Margaret; Cohen, Ross D.; Junkkarinen, Vesa T.; Lyons, R. W.

1997-01-01

We present HST/FOS spectra of the two bright images (A and B) of the gravitationally lensed QSO 0957+561 in the wavelength range 2200-3300 A. We find that the absorption system (Z(sub abs)) = 1.3911) near z(sub em) is a weak, damped Ly alpha system with strong Ly alpha absorption lines seen in both images. However, the H(I) column densities are different, with the line of sight to image A intersecting a larger column density. The continuum shapes of the two spectra differ in the sense that the flux level of image A increases more slowly toward shorter wavelengths than that of image B. We explain this as the result of differential reddening by dust grains in the damped Ly alpha absorber. A direct outcome of this explanation is a determination of the dust-to-gas ratio, k, in the damped Ly alpha system. We derive k = 0.55 + 0.18 for a simple 1/lambda extinction law and k = 0.31 + 0.10 for the Galactic extinction curve. For gravitationally lensed systems with damped Ly alpha absorbers, our method is a powerful tool for determining the values and dispersion of k, and the shapes of extinction curves, especially in the FUV and EUV regions. We compare our results with previous work.

21 CFR 866.5890 - Inter-alpha trypsin inhibitor immunological test system.

Code of Federal Regulations, 2013 CFR

2013-04-01

... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test... measure by immunochemical techniques the inter-alpha trypsin inhibitor (a protein) in serum and other body fluids. Measurement of inter-alpha trypsin inhibitor may aid in the diagnosis of acute bacterial...

21 CFR 866.5890 - Inter-alpha trypsin inhibitor immunological test system.

Code of Federal Regulations, 2011 CFR

2011-04-01

... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test... measure by immunochemical techniques the inter-alpha trypsin inhibitor (a protein) in serum and other body fluids. Measurement of inter-alpha trypsin inhibitor may aid in the diagnosis of acute bacterial...

21 CFR 866.5890 - Inter-alpha trypsin inhibitor immunological test system.

Code of Federal Regulations, 2012 CFR

2012-04-01

... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test... measure by immunochemical techniques the inter-alpha trypsin inhibitor (a protein) in serum and other body fluids. Measurement of inter-alpha trypsin inhibitor may aid in the diagnosis of acute bacterial...

21 CFR 866.5890 - Inter-alpha trypsin inhibitor immunological test system.

Code of Federal Regulations, 2014 CFR

2014-04-01

... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Immunological Test... measure by immunochemical techniques the inter-alpha trypsin inhibitor (a protein) in serum and other body fluids. Measurement of inter-alpha trypsin inhibitor may aid in the diagnosis of acute bacterial...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cornelio, Marcio F.; Oliveira, Marcos C. de

Employing the quantum Renyi {alpha} entropies as a measure of entanglement, we numerically find the violation of the strong superadditivity inequality for a system composed of four qubits and {alpha}>1. This violation gets smaller as {alpha}{yields}1 and vanishes for {alpha}=1 when the measure corresponds to the entanglement of formation. We show that the Renyi measure aways satisfies the standard monogamy of entanglement for {alpha}=2, and only violates a high-order monogamy inequality, in the rare cases in which the strong superadditivity is also violated. The sates numerically found where the violation occurs have special symmetries where both inequalities are equivalent. Wemore » also show that every measure satisfying monogamy for high-dimensional systems also satisfies the strong superadditivity inequality. For the case of Renyi measure, we provide strong numerical evidences that these two properties are equivalent.« less

Base-Pairing Systems Related to TNA: alpha-Threofuranosyl Oligonucleotides Containing Phosphoramidate Linkages

NASA Technical Reports Server (NTRS)

Meyer, Michael (Technical Monitor); Wu, Xiaolin; Guntha, Sreenivasulu; Ferenclc, Mathias; Krishnamurthy, Ramanarayanan; Eschenmoser, Albert

2002-01-01

(3'NH)- and (2'NH)-TNA, two isomeric phosphoramidate analogues of TNA (alpha-threofuranosyl-(3'-2') oligonucleotides), are shown to be efficient Watson-Crick base-pairing systems and to undergo intersystem crosspairing with TNA, RNA, and DNA.

Shifting the closed-loop spectrum in the optimal linear quadratic regulator problem for hereditary systems

NASA Technical Reports Server (NTRS)

Gibson, J. S.; Rosen, I. G.

1985-01-01

In the optimal linear quadratic regulator problem for finite dimensional systems, the method known as an alpha-shift can be used to produce a closed-loop system whose spectrum lies to the left of some specified vertical line; that is, a closed-loop system with a prescribed degree of stability. This paper treats the extension of the alpha-shift to hereditary systems. As infinite dimensions, the shift can be accomplished by adding alpha times the identity to the open-loop semigroup generator and then solving an optimal regulator problem. However, this approach does not work with a new approximation scheme for hereditary control problems recently developed by Kappel and Salamon. Since this scheme is among the best to date for the numerical solution of the linear regulator problem for hereditary systems, an alternative method for shifting the closed-loop spectrum is needed. An alpha-shift technique that can be used with the Kappel-Salamon approximation scheme is developed. Both the continuous-time and discrete-time problems are considered. A numerical example which demonstrates the feasibility of the method is included.

Shifting the closed-loop spectrum in the optimal linear quadratic regulator problem for hereditary systems

NASA Technical Reports Server (NTRS)

Gibson, J. S.; Rosen, I. G.

1987-01-01

In the optimal linear quadratic regulator problem for finite dimensional systems, the method known as an alpha-shift can be used to produce a closed-loop system whose spectrum lies to the left of some specified vertical line; that is, a closed-loop system with a prescribed degree of stability. This paper treats the extension of the alpha-shift to hereditary systems. As infinite dimensions, the shift can be accomplished by adding alpha times the identity to the open-loop semigroup generator and then solving an optimal regulator problem. However, this approach does not work with a new approximation scheme for hereditary control problems recently developed by Kappel and Salamon. Since this scheme is among the best to date for the numerical solution of the linear regulator problem for hereditary systems, an alternative method for shifting the closed-loop spectrum is needed. An alpha-shift technique that can be used with the Kappel-Salamon approximation scheme is developed. Both the continuous-time and discrete-time problems are considered. A numerical example which demonstrates the feasibility of the method is included.

Overview Of Suborbital Human Transportation Concept Alpha

NASA Astrophysics Data System (ADS)

Adirim, H.; Pilz, N.; Marini, M.; Hendrick, P.; Schmid, M.; Behr, R.; Barth, T.; Tarfeld, F.; Wiegand, A.; Charbonnier, D.; Haya Ramos, R.; Steeland, J.; Mack, A.

2011-05-01

Within the EC co-funded project FAST20XX (Future high-Altitude high-Speed Transport 20XX), the European suborbital passenger transportation system concept ALPHA (Airplane Launched PHoenix Aircraft), which shall be based to a maximum extent on existing technologies and capabilities, is currently being investigated as collaborative project by a European consortium under coordination of ESA. The ALPHA concept incorporates an air-launch from a carrier aircraft, which shall be used as first stage. The ALPHA vehicle shall be capable of transporting up to four passengers plus one pilot to an altitude of at least 100 km. The ALPHA vehicle is a down-scaled version of the suborbital space transportation concept Hopper, which was already deeply investigated within the European FESTIP System Study and the German ASTRA program including the successfully flown experimental landing demonstrator Phoenix. This approach has allowed the use of existing aerodynamic vehicle data and has led to the adaptation of the external Hopper/Phoenix configuration for ALPHA. In FESTIP and ASTRA, the Hopper configuration showed sufficient stability margins. Due to the geometric similarity of the ALPHA and Hopper vehicles, a trimable and flyable configuration could be derived by means of ALPHA flight trajectory calculations. In its current configuration, the ALPHA vehicle has a length of ca. 9 m and a gross take-off mass of ca. 3.5 Mg. The launch, staging and separation of ALPHA shall be performed either as internal air-launch from the cargo bay of the carrier aircraft, as under-wing air-launch or as towed air-launch. After separation from the carrier aircraft, the ALPHA vehicle ignites its onboard rocket propulsion system. Since conventional liquid and solid propulsion did not seem suitable for ALPHA due to Their high cost, limited safety and toxicity, a low-cost, “green” and non-hazardous hybrid propulsion system based on liquid nitrous oxide in combination with a solid polymer fuel was selected as baseline ALPHA propulsion. The general feasibility of hybrid propulsion for suborbital vehicle application with this propellant combination was already successfully demonstrated in the first reusable and privately-funded manned launch vehicle SpaceShipOne and consequently represents the solution with the lowest development risk for the investigated application. Due to the huge success of SpaceShipOne, the same type of hybrid propulsion is foreseen for Virgin Galactic’s SpaceShipTwo. ALPHA vehicle guidance will preferably be fully autonomous during the entire mission flight profile. The required technology for autonomous vehicle guidance can be adapted from the European RLV demonstrator Phoenix, which successfully demonstrated automated landing when it was dropped three times by a helicopter and landed precisely after a GPS-guided glide. This paper outlines the current status of the technology development work for ALPHA and has a special focus on aerodynamic and aerothermodynamic aspects of the concept.

Immunostimulatory effects of natural human interferon-alpha (huIFN-alpha) on carps Cyprinus carpio L.

PubMed

Watanuki, Hironobu; Chakraborty, Gunimala; Korenaga, Hiroki; Kono, Tomoya; Shivappa, R B; Sakai, Masahiro

2009-10-15

Human interferon-alpha (huIFN-alpha) is an important immunomodulatory substance used in the treatment and prevention of numerous infectious and immune-related diseases in animals. However, the immunostimulatory effects of huIFN-alpha in fish remain to be investigated. In the current study, the immune responses of the carp species Cyprinus carpio L. to treatment with huIFN-alpha were analyzed via measurement of superoxide anion production, phagocytic activity and the expression of cytokine genes including interleukin-1beta, tumor necrosis factor-alpha and interleukin 10. Low doses of huIFN-alpha were administered orally once a day for 3 days, and sampling was carried out at 1, 3 and 5 days post-treatment. Our results indicate that a low dose of huIFN-alpha significantly increased phagocytic activity and superoxide anion production in the carp kidney. The huIFN-alpha-treated fish also displayed a significant upregulation in cytokine gene expression. The current study demonstrates the stimulatory effects of huIFN-alpha on the carp immune system and highlights the immunomodulatory role of huIFN-alpha in fish.

The distribution of alpha elements in Andromeda dwarf galaxies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vargas, Luis C.; Geha, Marla C.; Tollerud, Erik J., E-mail: luis.vargas@yale.edu

We present alpha to iron abundance ratios for 226 individual red giant branch stars in nine dwarf galaxies of the Andromeda (M31) satellite system. The abundances are measured from the combined signal of Mg, Si, Ca, and Ti lines in Keck/DEIMOS medium-resolution spectra. This constitutes the first large sample of alpha abundance ratios measured in the M31 satellite system. The dwarf galaxies in our sample exhibit a variety of alpha abundance ratios, with the average values in each galaxy ranging from approximately solar ([α/Fe] ∼ + 0.0) to alpha-enhanced ([α/Fe] ∼ + 0.5). These variations do not show a correlationmore » with internal kinematics, environment, or stellar density. We confirm radial gradients in the iron abundance of two galaxies out of the five with sufficient data (NGC 185 and And II). There is only tentative evidence for an alpha abundance radial gradient in NGC 185. We homogeneously compare our results to the Milky Way classical dwarf spheroidals, finding evidence for wider variation in average alpha abundance. In the absence of chemical abundances for the M31 stellar halo, we compare to the Milky Way stellar halo. A stellar halo comprised of disrupted M31 satellites is too metal-rich and inconsistent with the Milky Way halo alpha abundance distribution even if considering only satellites with predominantly old stellar populations. The M31 satellite population provides a second system in which to study chemical abundances of dwarf galaxies and reveals a wider variety of abundance patterns than the Milky Way.« less

Analysis of Decentralized Variable Structure Control for Collective Search by Mobile Robots

DOE Office of Scientific and Technical Information (OSTI.GOV)

Feddema, J.; Goldsmith, S.; Robinett, R.

1998-11-04

This paper presents an analysis of a decentralized coordination strategy for organizing and controlling a team of mobile robots performing collective search. The alpha-beta coordination strategy is a family of collective search algorithms that allow teams of communicating robots to implicitly coordinate their search activities through a division of labor based on self-selected roIes. In an alpha-beta team. alpha agents are motivated to improve their status by exploring new regions of the search space. Beta a~ents are conservative, and reiy on the alpha agents to provide advanced information on favorable regions of the search space. An agent selects its currentmore » role dynamically based on its current status value relative to the current status values of the other team members. Status is determined by some function of the agent's sensor readings, and is generally a measurement of source intensity at the agent's current location. Variations on the decision rules determining alpha and beta behavior produce different versions of the algorithm that lead to different global properties. The alpha-beta strategy is based on a simple finite-state machine that implements a form of Variable Structure Control (VSC). The VSC system changes the dynamics of the collective system by abruptly switching at defined states to alternative control laws . In VSC, Lyapunov's direct method is often used to design control surfaces which guide the system to a given goal. We introduce the alpha-beta aIgorithm and present an analysis of the equilibrium point and the global stability of the alpha-beta algorithm based on Lyapunov's method.« less

Analysis of decentralized variable structure control for collective search by mobile robots

NASA Astrophysics Data System (ADS)

Goldsmith, Steven Y.; Feddema, John T.; Robinett, Rush D., III

1998-10-01

This paper presents an analysis of a decentralized coordination strategy for organizing and controlling a team of mobile robots performing collective search. The alpha- beta coordination strategy is a family of collective search algorithms that allow teams of communicating robots to implicitly coordinate their search activities through a division of labor based on self-selected roles. In an alpha- beta team, alpha agents are motivated to improve their status by exploring new regions of the search space. Beta agents are conservative, and rely on the alpha agents to provide advanced information on favorable regions of the search space. An agent selects its current role dynamically based on its current status value relative to the current status values of the other team members. Status is determined by some function of the agent's sensor readings, and is generally a measurement of source intensity at the agent's current location. Variations on the decision rules determining alpha and beta behavior produce different versions of the algorithm that lead to different global properties. The alpha-beta strategy is based on a simple finite-state machine that implements a form of Variable Structure Control (VSC). The VSC system changes the dynamics of the collective system by abruptly switching at defined states to alternative control laws. In VSC, Lyapunov's direct method is often used to design control surfaces which guide the system to a given goal. We introduce the alpha- beta algorithm and present an analysis of the equilibrium point and the global stability of the alpha-beta algorithm based on Lyapunov's method.

cap alpha. -Methylglucoside satisfies only Na/sup +/-dependent transport system of intestinal epithelium

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kimmich, G.A.; Randles, J.

1981-01-01

The unidirectional influx of ..cap alpha..-methylglucoside (..cap alpha..-MG) by isolated chicken intestinal epithelial cells is 98% inhibited by phlorizin. The remaining 2% of the total influx occurs in the absence of Na/sup +/, is not sensitive to phloretin, and is equal to the diffusional entry rate for 2-deoxyglucose. The glucoside is much more strongly accumulated (75-fold) than 3-O-methylglucose (3-OMG) (10-fold). Inhibitors of the serosal sugar carrier (phloretin, cytochalasin B, theophylline, and flavanoids) do not enhance ..cap alpha..-MG accumulation. It is concluded that the glycoside is not a substrate for the intestinal serosal transport system. Steady-state gradients of the sugar canmore » be represented accurately by a concentrative, phlorizin-sensitive system that is opposed by a diffusional efflux process.« less

Cryogenic Microcalorimeter System for Ultra-High Resolution Alpha-Particle Spectrometry

NASA Astrophysics Data System (ADS)

Croce, M. P.; Bacrania, M. K.; Hoover, A. S.; Rabin, M. W.; Hoteling, N. J.; LaMont, S. P.; Plionis, A. A.; Dry, D. E.; Ullom, J. N.; Bennett, D. A.; Horansky, R. D.; Kotsubo, V.; Cantor, R.

2009-12-01

Microcalorimeters have been shown to yield unsurpassed energy resolution for alpha spectrometry, up to 1.06 keV FWHM at 5.3 MeV. These detectors use a superconducting transition-edge sensor (TES) to measure the temperature change in an absorber from energy deposited by an interacting alpha particle. Our system has four independent detectors mounted inside a liquid nitrogen/liquid helium cryostat. An adiabatic demagnetization refrigerator (ADR) cools the detector stage to its operating temperature of 80 mK. Temperature regulation with ˜15-μK peak-to-peak variation is achieved by PID control of the ADR. The detectors are voltage-biased, and the current signal is amplified by a commercial SQUID readout system and digitized for further analysis. This paper will discuss design and operation of our microcalorimeter alpha-particle spectrometer, and will show recent results.

STUDIES ON MAMMALIAN AND HUMAN PYRUVATE AND ALPHA-KETOGLUTARATE DEHYDROGENATION COMPLEXES.

DTIC Science & Technology

Enzyme systems that catalyze a coenzyme A- and nicotinamide adenine dinucleotide-linked oxidative decarboxylation of pyruvate and alpha - ketoglutarate ...The pig heart pyruvate dehydrogenase complex was strongly inhibited by EDTA at low concentration, but the pig heart alpha - ketoglutarate ...On the oxidative decarboxylation of alpha -keto acids in pig heart complexes, Ca(2+) was strongly stimulatory to the same or more extent than Mg(2

Heritable major histocompatibility complex class II-associated differences in production of tumor necrosis factor. alpha. : Relevance to genetic predisposition to systemic lupus erythematosus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jacob, C.O.; Fronek, Z.; Koo, M.

1990-02-01

The authors report on the production of tumor necrosis factor (TNF)-{alpha} and TNF-{beta} by mitogen-activated peripheral blood lymphocytes or enriched monocyte subpopulations from human leukocyte antigen (HLA)-typed healthy subjects. The results indicate that HLA-DR2- and DQw1-positive donors frequently exhibit low production of TNF-{alpha}, whereas DR3- and DR4-positive subjects show high levels of TNF-{alpha} production. No correlation between TNF-{alpha} levels and HLA-A, -B, and -C genotype was found. The relevance of this quantitative polymorphism to the genetic predisposition to lupus nephritis in systemic lupus erythematosus (SLE) patients was investigated. DR2, DQw1-positive SLE patients show low levels of TNF-{alpha} inducibility; this genotypemore » is also associated with an increased incidence of lupus nephritis. DR3-positive SLE patients, on the other hand, are not predisposed to nephritis, and these patients have high TNF-{alpha} production. DR4 haplotype is associated with high TNF-{alpha} inducibility and is negatively correlated with lupus nephritis. These data may help explain the strong association between HLA-DR2, DQw1 in SLE patients and their susceptibility to nephritis.« less

Small molecular antioxidants effectively protect from PUVA-induced oxidative stress responses underlying fibroblast senescence and photoaging.

PubMed

Briganti, Stefania; Wlaschek, Meinhard; Hinrichs, Christina; Bellei, Barbara; Flori, Enrica; Treiber, Nicolai; Iben, Sebastian; Picardo, Mauro; Scharffetter-Kochanek, Karin

2008-09-01

Exposure of human fibroblasts to 8-methoxypsoralen plus ultraviolet-A irradiation (PUVA) results in stress-induced cellular senescence in fibroblasts. We here studied the role of the antioxidant defense system in the accumulation of reactive oxygen species (ROS) and the effect of the antioxidants alpha-tocopherol, N-acetylcysteine, and alpha-lipoic acid on PUVA-induced cellular senescence. PUVA treatment induced an immediate and increasing generation of intracellular ROS. Supplementation of PUVA-treated fibroblasts with alpha-tocopherol (alpha-Toc), N-acetylcysteine (NAC), or alpha-lipoic acid (alpha-LA) abrogated the increased ROS generation and rescued fibroblasts from the ROS-dependent changes into the cellular senescence phenotype, such as cytoplasmic enlargement, enhanced expression of senescence-associated-beta-galactosidase and matrix-metalloproteinase-1, hallmarks of photoaging and intrinsic aging. PUVA treatment disrupted the integrity of cellular membranes and impaired homeostasis and function of the cellular antioxidant system with a significant decrease in glutathione and hydrogen peroxide-detoxifying enzymes activities. Supplementation with NAC, alpha-LA, and alpha-Toc counteracted these changes. Our data provide causal evidence that (i) oxidative stress due to an imbalance in the overall cellular antioxidant capacity contributes to the induction and maintenance of the PUVA-induced fibroblast senescence and that (ii) low molecular antioxidants protect effectively against these deleterious alterations.

Alpha power indexes task-related networks on large and small scales: A multimodal ECoG study in humans and a non-human primate.

PubMed

de Pesters, A; Coon, W G; Brunner, P; Gunduz, A; Ritaccio, A L; Brunet, N M; de Weerd, P; Roberts, M J; Oostenveld, R; Fries, P; Schalk, G

2016-07-01

Performing different tasks, such as generating motor movements or processing sensory input, requires the recruitment of specific networks of neuronal populations. Previous studies suggested that power variations in the alpha band (8-12Hz) may implement such recruitment of task-specific populations by increasing cortical excitability in task-related areas while inhibiting population-level cortical activity in task-unrelated areas (Klimesch et al., 2007; Jensen and Mazaheri, 2010). However, the precise temporal and spatial relationships between the modulatory function implemented by alpha oscillations and population-level cortical activity remained undefined. Furthermore, while several studies suggested that alpha power indexes task-related populations across large and spatially separated cortical areas, it was largely unclear whether alpha power also differentially indexes smaller networks of task-related neuronal populations. Here we addressed these questions by investigating the temporal and spatial relationships of electrocorticographic (ECoG) power modulations in the alpha band and in the broadband gamma range (70-170Hz, indexing population-level activity) during auditory and motor tasks in five human subjects and one macaque monkey. In line with previous research, our results confirm that broadband gamma power accurately tracks task-related behavior and that alpha power decreases in task-related areas. More importantly, they demonstrate that alpha power suppression lags population-level activity in auditory areas during the auditory task, but precedes it in motor areas during the motor task. This suppression of alpha power in task-related areas was accompanied by an increase in areas not related to the task. In addition, we show for the first time that these differential modulations of alpha power could be observed not only across widely distributed systems (e.g., motor vs. auditory system), but also within the auditory system. Specifically, alpha power was suppressed in the locations within the auditory system that most robustly responded to particular sound stimuli. Altogether, our results provide experimental evidence for a mechanism that preferentially recruits task-related neuronal populations by increasing cortical excitability in task-related cortical areas and decreasing cortical excitability in task-unrelated areas. This mechanism is implemented by variations in alpha power and is common to humans and the non-human primate under study. These results contribute to an increasingly refined understanding of the mechanisms underlying the selection of the specific neuronal populations required for task execution. Copyright © 2016 Elsevier Inc. All rights reserved.

IRAF and STSDAS under the new ALPHA architecture

NASA Technical Reports Server (NTRS)

Zarate, N. R.

1992-01-01

Digital's next generation RISC architecture, known as ALPHA, presents many IRAF system portability questions and challenges to both site managers and end users. DEC promises to support the ULTRIX, VMS, and OSF/1 operating systems, which should allow IRAF to be ported to the new architecture at either the program executable level (using VEST), or at the source level, where IRAF can be tuned for greater performance. These notes highlight some of the details of porting IRAF to OpenVMS on the ALPHA architecture.

The Hottest Horizontal-Branch Stars in Omega Centauri: Late Hot Flasher vs. Helium Enrichment

NASA Technical Reports Server (NTRS)

Moehler, S.; Dreizler, S.; Lanz, T.; Bono, G.; Sweigart, A V.; Calamida, A.; Monelli, M.; Nonino, M.

2007-01-01

UV observations of some massive globular clusters uncovered a significant population of very hot stars below the hot end of the horizontal branch (HB), the so-called blue hook stars. This feature might be explained either by the late hot flasher scenario here stars experience the helium flash while on the white dwarf cooling curve or by the helium-rich sub-population recently postulated to exist in some clusters. Spectroscopic analyses of blue hook stars in omega Cen and NGC 2808 support the late hot flasher scenario, but the stars contain much less helium than expected and the predicted C, N enrichment could not be verified from existing data. We want to determine effective temperatures, surface gravities and abundances of He, C, N in blue hook and canonical extreme horizontal branch (EHB) star candidates. Moderately high resolution spectra of stars at the hot end of the blue horizontal branch in the globular cluster omega Cen were analysed for atmospheric parameters (T(sub eff), log g) and abundances using LTE and Non-LTE model atmospheres. In the temperature range 30,000 K to 50,000 K we find that 37% of our stars are helium-poor (log nHe/nH less than -2), 49% have solar helium abundance within a factor of 3 (-1.5 less than or equal to log nHe/nH less than or equal to -0.5) and 14% are helium rich (log nHe/nH greater than -0.4). We also find carbon enrichment in step with helium enrichment, with a maximum carbon enrichment of 3% by mass. At least 30% of the hottest HB stars in omega Centauri show helium abundances well above the predictions from the helium enrichment scenario (Y = 0.42 corresponding to log nHe/nH approximately equal to -0.74). In addition the most helium-rich stars show strong carbon enrichment as predicted by the late hot flasher scenario. We conclude that the helium-rich HB stars in omega Cen cannot be explained solely by the helium-enrichment scenario invoked to explain the blue main sequence.

Exoplanet modelling with the Met Office Unified Model

NASA Astrophysics Data System (ADS)

Boutle, Ian; Lines, Stefan; Mayne, Nathan; Lee, Graham; Helling, Christiane; Drummond, Ben; Manners, James; Goyal, Jayesh; Lambert, Hugo; Acreman, David; Earnshaw, Paul; Amundsen, David; Baraffe, Isabelle

2017-04-01

This talk will present an overview of work being done to adapt the Unified Model, one of the most sophisticated weather and climate models of this planet, into a flexible planet simulator for use in the study of any exoplanet. We will focus on two current projects: Clouds in hot Jupiter atmospheres - recent HST observations have revealed a continuum in atmospheric composition from cloudy to clear skies. The presence of clouds is inferred from a grey opacity in the near-IR that mutes key absorption features in the transmission spectra. Unlike the L-T Brown Dwarf sequence, this transition does not correlate well with equilibrium temperature, suggesting that a cloud formation scheme more comprehensive than simply considering the condensation temperature needed for homogenous cloud growth, is required. In our work, we conduct 3D simulations of cloud nucleation, growth, advection, evaporation and gravitational settling in the atmospheres of HD209458b and HD189733 using the kinetic and mixed-grain cloud formation code DIHRT, coupled to the Unified Model. We explore cloud composition, vertical structure and particle sizes, as well as highlighting the importance of the strong atmospheric dynamics seen in tidally locked hot Jupiters on the evolution and distribution of the cloud. Climate of Proxima B - we present results of simulations of the climate of the newly discovered planet Proxima Centauri B, examining the responses of both an `Earth-like' atmosphere and simplified nitrogen and trace carbon dioxide atmosphere to the radiation likely received. Overall, our results are in agreement with previous studies in suggesting Proxima Centauri B may well have surface temperatures conducive to the presence of liquid water. Moreover, we have expanded the parameter regime over which the planet may support liquid water to higher values of eccentricity and lower incident fluxes, guided by observational constraints. This increased parameter space arises because of the low sensitivity of the planet to changes in stellar flux, a consequence of the stellar spectrum and orbital configuration. Finally, we have produced high resolution planetary emission and reflectance spectra, and highlight signatures of gases vital to the evolution of life on Earth (oxygen, ozone and carbon dioxide).

First Results of Using a UVTron Flame Sensor to Detect Alpha-Induced Air Fluorescence in the UVC Wavelength Range.

PubMed

Crompton, Anita J; Gamage, Kelum A A; Bell, Steven; Wilson, Andrew P; Jenkins, Alex; Trivedi, Divyesh

2017-11-29

In this work, a robust stand-off alpha detection method using the secondary effects of alpha radiation has been sought. Alpha particles ionise the surrounding atmosphere as they travel. Fluorescence photons produced as a consequence of this can be used to detect the source of the alpha emissions. This paper details experiments carried out to detect this fluorescence, with the focus on photons in the ultraviolet C (UVC) wavelength range (180-280 nm). A detector, UVTron R9533 (Hamamatsu, 325-6, Sunayama-cho, Naka-ku, Hamamatsu City, Shizuoka Pref., 430-8587, Japan), designed to detect the UVC emissions from flames for fire alarm purposes, was tested in various gas atmospheres with a 210 Po alpha source to determine if this could provide an avenue for stand-off alpha detection. The results of the experiments show that this detector is capable of detecting alpha-induced air fluorescence in normal indoor lighting conditions, as the interference from daylight and artificial lighting is less influential on this detection system which operates below the UVA and UVB wavelength ranges (280-315 nm and 315-380 nm respectively). Assuming a standard 1 r 2 drop off in signal, the limit of detection in this configuration can be calculated to be approximately 240 mm, well beyond the range of alpha-particles in air, which indicates that this approach could have potential for stand-off alpha detection. The gas atmospheres tested produced an increase in the detector count, with xenon having the greatest effect with a measured 52% increase in the detector response in comparison to the detector response in an air atmosphere. This type of alpha detection system could be operated at a distance, where it would potentially provide a more cost effective, safer, and faster solution in comparison with traditional alpha detection methods to detect and characterise alpha contamination in nuclear decommissioning and security applications.

First Results of Using a UVTron Flame Sensor to Detect Alpha-Induced Air Fluorescence in the UVC Wavelength Range

PubMed Central

Crompton, Anita J.; Wilson, Andrew P.; Jenkins, Alex; Trivedi, Divyesh

2017-01-01

In this work, a robust stand-off alpha detection method using the secondary effects of alpha radiation has been sought. Alpha particles ionise the surrounding atmosphere as they travel. Fluorescence photons produced as a consequence of this can be used to detect the source of the alpha emissions. This paper details experiments carried out to detect this fluorescence, with the focus on photons in the ultraviolet C (UVC) wavelength range (180–280 nm). A detector, UVTron R9533 (Hamamatsu, 325-6, Sunayama-cho, Naka-ku, Hamamatsu City, Shizuoka Pref., 430-8587, Japan), designed to detect the UVC emissions from flames for fire alarm purposes, was tested in various gas atmospheres with a 210Po alpha source to determine if this could provide an avenue for stand-off alpha detection. The results of the experiments show that this detector is capable of detecting alpha-induced air fluorescence in normal indoor lighting conditions, as the interference from daylight and artificial lighting is less influential on this detection system which operates below the UVA and UVB wavelength ranges (280–315 nm and 315–380 nm respectively). Assuming a standard 1r2 drop off in signal, the limit of detection in this configuration can be calculated to be approximately 240 mm, well beyond the range of alpha-particles in air, which indicates that this approach could have potential for stand-off alpha detection. The gas atmospheres tested produced an increase in the detector count, with xenon having the greatest effect with a measured 52% increase in the detector response in comparison to the detector response in an air atmosphere. This type of alpha detection system could be operated at a distance, where it would potentially provide a more cost effective, safer, and faster solution in comparison with traditional alpha detection methods to detect and characterise alpha contamination in nuclear decommissioning and security applications. PMID:29186051

Effects of alpha-lipoic acid on spatial learning and memory, oxidative stress, and central cholinergic system in a rat model of vascular dementia.

PubMed

Zhao, Ran-Ran; Xu, Fei; Xu, Xiao-Chen; Tan, Guo-Jun; Liu, Liang-Min; Wu, Ning; Zhang, Wen-Zhong; Liu, Ji-Xiang

2015-02-05

Brain oxidative stress due to chronic cerebral hypoperfusion was considered to be the major risk factor in the pathogenesis of vascular dementia. In this study, we investigated the protective efficacy of alpha-lipoic acid, an antioxidant, against vascular dementia in rats, as well as the potential mechanism. Bilateral common carotid arteries occlusion (BCCAO) induced severe cognitive deficits tested by Morris water maze (MWM), along with oxidative stress and disturbance of central cholinergic system. However, administration of alpha-lipoic acid (50mg/kg, i.p.) for 28 days significantly restored cognitive deficits induced by BCCAO. Biochemical determination revealed that alpha-lipoic acid markedly decreased the production of malondialdehyde (MDA) and the generation of reactive oxidative species (ROS), and increased the level of reduced glutathione (GSH) in the hippocampal tissue. Additionally, alpha-lipoic acid raised the level of acetylcholine (ACh) and choline acetyltransferase (ChAT) and decreased the activity of acetycholinesterase (AChE) in the hippocampus. These results indicated that treatment with alpha-lipoic acid significantly improved behavioral alterations, protected against oxidative stress, and restored central cholinergic system in the rat model of vascular dementia induced by BCCAO. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

New Complexity-Building Reactions of Alpha-Keto Esters

NASA Astrophysics Data System (ADS)

Bartlett, Samuel L.

I. Introduction: Importance of Asymmetric Catalysis and the Reactivity Patterns of alpha-Keto Esters. II. Synthesis of Complex Tertiary Glycolates by Enantioconvergent Arylation of Stereochemically Labile alpha-Keto Esters. Enantioconvergent arylation reactions of boronic acids and racemic ?-stereogenic alpha-keto esters have been developed. The reactions are catalyzed by a chiral (diene)Rh(I) complex and provide a wide array of beta-stereogenic tertiary aryl glycolate derivatives with high levels of diastereo- and enantioselectivity. Racemization studies employing a series of sterically differentiated tertiary amines suggest that the steric nature of the amine base additive exerts a significant influence on the rate of substrate racemization. III. Palladium-Catalyzed beta-Arylation of alpha-Keto Esters . A catalyst system derived from commercially available Pd2(dba) 3 and PtBu3 has been applied to the coupling of alpha-keto ester enolates and aryl bromides. The reaction provides access to an array of beta-stereogenic alpha-keto ester derivatives. When the air stable ligand precursor PtBu 3˙HBF4 is employed, the reaction can be carried out without use of a glovebox. The derived products are of broad interest given the prevalence of the alpha-keto acid substructure in biologically important molecules. IV. Catalytic Enantioselective [3+2] Cycloaddition of alpha-Keto Ester Enolates and Nitrile Oxides. An enantioselective [3+2] cycloaddition reaction between nitrile oxides and transiently generated enolates of alpha-keto esters has been developed. The catalyst system was found to be compatible with in situ nitrile oxide generation conditions. A versatile array of nitrile oxides and alpha-keto esters could participate in the cycloaddition, providing novel 5-hydroxy-2-isoxazolines in high chemical yield with high levels of diastereo- and enantioselectivity. Notably, the optimal reaction conditions circumvented concurrent reaction via O-imidoylation and hetero-[3+2] pathways.

Effects of cross-correlated noises on the relaxation time of the bistable system

NASA Astrophysics Data System (ADS)

Xie, Chong-Wei; Mei, Dong-Cheng

2003-11-01

The stationary correlation function and the associated relaxation time for a general system driven by cross-correlated white noises are derived, by virtue of a Stratonovich-like ansatz. The effects of correlated noises on the relaxation time of a bistable kinetic model coupled to an additive and a multiplicative white noises are studied. It is proved that for small fluctuations the relaxation time Tc as a function of lambda (the correlated intensity between noises) exhibits very different behaviours for alphaD (alpha and D, respectively, stand for the intensities of additive and multiplicative noises). When alpha>D, Tc increases with increasing lambda. But when alpha

Flares from small to large: X-ray spectroscopy of Proxima Centauri with XMM-Newton

NASA Astrophysics Data System (ADS)

Güdel, M.; Audard, M.; Reale, F.; Skinner, S. L.; Linsky, J. L.

2004-03-01

We report results from a comprehensive study of the nearby M dwarf Proxima Centauri with the XMM-Newton satellite, using simultaneously its X-ray detectors and the Optical Monitor with its U band filter. We find strongly variable coronal X-ray emission, with flares ranging over a factor of 100 in peak flux. The low-level emission is found to be continuously variable on at least three time scales (a slow decay of several hours, modulation on a time scale of 1 hr, and weak flares with time scales of a few minutes). Several weak flares are characteristically preceded by an optical burst, compatible with predictions from standard solar flare models. We propose that the U band bursts are proxies for the elusive stellar non-thermal hard X-ray bursts suggested from solar observations. In the course of the observation, a very large X-ray flare started and was observed essentially in its entirety. Its peak luminosity reached 3.9× 1028 erg s-1 [0.15-10 keV], and the total X-ray energy released in the same band is derived to be 1.5× 1032 ergs. This flare has for the first time allowed to measure significant density variations across several phases of the flare from X-ray spectroscopy of the O VII He-like triplet; we find peak densities reaching up to 4× 1011 cm-3 for plasma of about 1-5 MK. Abundance ratios show little variability in time, with a tendency of elements with a high first ionization potential to be overabundant relative to solar photospheric values. Using Fe XVII lines with different oscillator strengths, we do not find significant effects due to opacity during the flare, indicating that large opacity increases are not the rule even in extreme flares. We model the large flare in terms of an analytic 2-Ribbon flare model and find that the flaring loop system should have large characteristic sizes (≈ 1R*) within the framework of this simplistic model. These results are supported by full hydrodynamic simulations. Comparing the large flare to flares of similar size occurring much more frequently on more active stars, we propose that the X-ray properties of active stars are a consequence of superimposed flares such as the example analyzed in this paper. Since larger flares produce hotter plasma, such a model also explains why, during episodes of low-level emission, more active stars show hotter plasma than less active stars. Based on observations obtained with XMM-Newton, an ESA science mission with instruments and contributions directly funded by ESA Member States and the USA (NASA).

[Immune diagnostics of disorders in the IFN-alpha/NK-cell system in patients with frequently recurrent herpes simplex].

PubMed

Karsonova, A V; Shulzhenko, A E; Karaulov, A V

2014-01-01

Study of features of NK-cell response to the effect of recombinant IFN-alpha in complex with evaluation of the ability to synthesize inherent IFN-alpha in patients with frequently recurrent herpes simplex (FRHS). 48 patients with genital (n = 31), labial (n = 10) and mixed localization (n = 7) FRHS diagnosis were observed. 31 healthy donors composed the control group. MC were cultivated in the presence of a recombinant human IFN-alpha2b at the concentration of 10, 100 and 1000 U/ml for 24 hours. NK-cell response to the effect of IFN-alpha was evaluated after 24 hours using flow cytometry by degranulation reaction and in the NK-activity test. IFN-alpha synthesis was evaluated in HSV-1, HSV-2 and Newcastle disease virus stimulated cell supernatants by EIA method. Patients with FRHS were established to be a heterogeneous group by parameters in the IFN-alpha/NK-cell cytotoxicity system. 2 types of NK-cell response to the stimulation by recombinant IFN-alpha were identified. Type A is characterized by a decrease of NK-cell response to IFN-alpha in the remission phase and does not have this defect in the exacerbation phase. Synthesis of inherent IFN-alpha in response to viral inductors for type A was comparable with the response in healthy donors in both phases. On the contrary type B having normal sensitivity of NK-cells to IFN-alpha in the remission phase is characterized by a decrease of this parameter in the exacerbation phase for more than 3 times. Synthesis of inherent IFN-alpha in response to viral inductors during type B is increased in the remission phase and decreased in the exacerbation phase. During immune-correcting therapy of FRHS a personalized approach taking into account features of NK-cell response to IFN-alpha is necessary, because types A and B have principal differences by cytotoxicity parameters of NK-cells and their change under the effect of IFN-alpha, as well as by parameters of IFN-alpha synthesis in response to viral inductors at various phases of the clinical process.

Real-time alpha monitoring of a radioactive liquid waste stream at Los Alamos National Laboratory

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johnson, J.D.; Whitley, C.R.; Rawool-Sullivan, M.

1995-12-31

This poster display concerns the development, installation, and testing of a real-time radioactive liquid waste monitor at Los Alamos National Laboratory (LANL). The detector system was designed for the LANL Radioactive Liquid Waste Treatment Facility so that influent to the plant could be monitored in real time. By knowing the activity of the influent, plant operators can better monitor treatment, better segregate waste (potentially), and monitor the regulatory compliance of users of the LANL Radioactive Liquid Waste Collection System. The detector system uses long-range alpha detection technology, which is a nonintrusive method of characterization that determines alpha activity on themore » liquid surface by measuring the ionization of ambient air. Extensive testing has been performed to ensure long-term use with a minimal amount of maintenance. The final design was a simple cost-effective alpha monitor that could be modified for monitoring influent waste streams at various points in the LANL Radioactive Liquid Waste Collection System.« less

Low-Cost alpha Alane for Hydrogen Storage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fabian, Tibor; Petrie, Mark; Crouch-Baker, Steven

This project was directed towards the further development of the Savannah River National Laboratory (SRNL) lab-scale electrochemical synthesis of the hydrogen storage material alpha-alane and Ardica Technologies-SRI International (SRI) chemical downstream processes that are necessary to meet DoE cost metrics and transition alpha-alane synthesis to an industrial scale. Ardica has demonstrated the use of alpha-alane in a fuel-cell system for the U.S. Army WFC20 20W soldier power system that has successfully passed initial field trials with individual soldiers. While alpha-alane has been clearly identified as a desirable hydrogen storage material, cost-effective means for its production and regeneration on a scalemore » of use applicable to the industry have yet to be established. We focused on three, principal development areas: 1. The construction of a comprehensive engineering techno-economic model to establish the production costs of alpha-alane by both electrochemical and chemical routes at scale. 2. The identification of critical, cost-saving design elements of the electrochemical cell and the quantification of the product yields of the primary electrochemical process. A moving particle-bed reactor design was constructed and operated. 3. The experimental quantification of the product yields of candidate downstream chemical processes necessary to produce alpha-alane to complete the most cost-effective overall manufacturing process. Our techno-economic model shows that under key assumptions most 2015 and 2020 DOE hydrogen storage system cost targets for low and medium power can be achieved using the electrochemical alane synthesis process. To meet the most aggressive 2020 storage system cost target, $1/g, our model indicates that 420 metric tons per year (MT/y) production of alpha-alane is required. Laboratory-scale experimental work demonstrated that the yields of two of the three critical component steps within the overall “electrochemical process” were sufficiently high to meet this production target. In the case of the yield of the third step, the crystallization of alpha-alane from the primary alane-related product of the electrochemical reaction, further development is required.« less

Development of Flight Slit-Jaw Optics for Chromospheric Lyman-Alpha SpectroPolarimeter

NASA Technical Reports Server (NTRS)

Kubo, Masahito; Suematsu, Yoshinori; Kano, Ryohei; Bando, Takamasa; Hara, Hirohisa; Narukage, Noriyuki; Katsukawa, Yukio; Ishikawa, Ryoko; Ishikawa, Shin-nosuke; Kobiki, Toshihiko;

Defect production in nonlinear quench across a quantum critical point.

PubMed

Sen, Diptiman; Sengupta, K; Mondal, Shreyoshi

2008-07-04

We show that the defect density n, for a slow nonlinear power-law quench with a rate tau(-1) and an exponent alpha>0, which takes the system through a critical point characterized by correlation length and dynamical critical exponents nu and z, scales as n approximately tau(-alphanud/(alphaznu+1)) [n approximately (alphag((alpha-1)/alpha)/tau)(nud/(znu+1))] if the quench takes the system across the critical point at time t=0 [t=t(0) not = 0], where g is a nonuniversal constant and d is the system dimension. These scaling laws constitute the first theoretical results for defect production in nonlinear quenches across quantum critical points and reproduce their well-known counterpart for a linear quench (alpha=1) as a special case. We supplement our results with numerical studies of well-known models and suggest experiments to test our theory.

Cryogenic microcalorimeter system for ultra-high resolution alpha-particle spectrometry

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rabin, Michael W; Hoover, Andrew S; Bacrania, Mnesh K

2009-01-01

Microcalorimeters have been shown to yield unsurpassed energy resolution for alpha spectrometry, up to 1.06 keV FWHM at 5.3 MeV. These detectors use a superconducting transition-edge sensor (TES) to measure the temperature change in an absorber from energy deposited by an interacting alpha particle. Our system has four independent detectors mounted inside a liquid nitrogen/liquid helium cryostat. An adiabatic demagnetization refrigerator (ADR) cools the detector stage to its operating temperature of 80 mK. Temperature regulation with {approx}15 uK peak-to-peak variation is achieved by PID control of the ADR. The detectors are voltage-biased, and the current signal is amplified by amore » commercial SQUID readout system and digitized for further analysis, This paper will discuss design and operation of our microcalorimeter alpha spectrometer, and will show recent results.« less

Hyperglycemia-conditioned increase in alpha-2-macroglobulin in healthy normal subjects: a phenomenon correlated with deficient antithrombin III activity.

PubMed

Ceriello, A; Quatraro, A; Dello Russo, P; Marchi, E; Barbanti, M; Giugliano, D

1989-01-01

Induced hyperglycemia in normal subjects increases alpha 2-macroglobulin (alpha 2M) activity and alpha 2M concentration and reduces antithrombin III (ATIII) activity, while it does not affect ATIII plasma concentration. Hyperglycemia-determined variations in ATIII activity and alpha 2M molecules are correlated in an inverse and parallel fashion. A compensatory role for the increase in alpha 2M in the regulation of the coagulation system may be hypothesized. Moreover, these data provide evidence that hyperglycemia may decrease, directly, the biological function of some proteins and may influence the levels of some risk factors for the development of complications in diabetes.

Formation and self-organization kinetics of alpha-CD/PEO-based pseudo-polyrotaxanes in water. A specific behavior at 30 degrees C.

PubMed

Travelet, Christophe; Schlatter, Guy; Hébraud, Pascal; Brochon, Cyril; Lapp, Alain; Hadziioannou, Georges

2009-08-04

alpha-Cyclodextrins (alpha-CDs) have the ability to form inclusion complexes with poly(ethylene oxide) (PEO) polymer chains. These pseudo-polyrotaxanes (PPRs) can be obtained by quenching an alpha-CD/PEO mixture in water from 70 degrees C down to a lower temperature (typically in the range from 5 to 30 degrees C) thanks to favorable interactions between alpha-CD cavities and PEO chains. Moreover, starting from a liquid alpha-CD/PEO mixture at a total mass fraction of 15% w/w at 70 degrees C, the formation of PPRs with time at a lower temperature induces a white physical gel with time, and phase separation is observed. We established that PPR molecules are exclusively found in the precipitated phase although unthreaded alpha-CD molecules and unthreaded PEO chains are in the liquid phase. At 30 degrees C, the physical gel formation is much slower than at 5 degrees C. At 30 degrees C, we established that, in a first step, alpha-CDs thread onto PEO chains, forming PPR molecules which are not in good solvent conditions in water. At a higher length scale, rapid aggregation of the PPR molecules occurs, and threaded alpha-CD-based nanocylinders form (cylinder length L = 5.7 nm and cylinder radius R = 4.7 nm). At a higher length scale, alpha-CD-based nanocylinders associate in a Gaussian way, engendering the formation of precipitated domains which are responsible for the high turbidity of the studied system. At the end of this first step (i.e., after 20 min), the system still remains liquid and the PPRs are totally formed. Then, in a second step (i.e., after 150 min), the system undergoes its reorganization characterized by a compacity increase of the precipitated domains and forms a physical gel. We found that PPRs are totally formed after 20 min at 30 degrees C and that the system stays in a nongel state up to 150 min. This opens new perspectives regarding the PPR chemical modification: between these two characteristic times, we can easily envisage an efficient chemical modification of the PPR molecules in water, as for instance an end-capping reaction leading to the synthesis of polyrotaxanes.

Deficiency of a alpha-1-antitrypsin influences systemic iron homeostasis

EPA Science Inventory

Abstract Background: There is evidence that proteases and anti-proteases participate in the iron homeostasis of cells and living systems. We tested the postulate that alpha-1 antitrypsin (A1AT) polymorphism and the consequent deficiency of this anti-protease in humans are asso...

Neuroendocrine mediators up-regulate alpha1b- and alpha1d-adrenergic receptor subtypes in human monocytes.

PubMed

Rouppe van der Voort, C; Kavelaars, A; van de Pol, M; Heijnen, C J

1999-03-01

Beta2- and alpha2-adrenergic receptors (AR) are thought to be the main AR subtypes to exert the effects of catecholamines on the immune system. However, in the present study, we demonstrate that another subtype of AR can be induced in human monocytes. Expression of alpha1b- and alpha1d-AR mRNA can be obtained by culturing freshly isolated human peripheral blood monocytes with the neuroendocrine mediators dexamethasone or the beta2-AR agonist terbutaline. Using the human monocytic cell line THP-1, we demonstrate that increased levels of alpha1b- and alpha1d-mRNA are accompanied by increased levels of receptor protein as determined by Western blot analysis and radioligand binding assays. This study describes for the first time regulated expression of alpha1-AR subtypes in human monocytes.

Progesterone biotransformation by plant cell suspension cultures.

PubMed Central

Yagen, B; Gallili, G E; Mateles, R I

1978-01-01

Progesterone was converted to 5alpha-pregnane-3alpha-ol-20-one, delta4-pregnene-20alpha-ol-3-one, delta4-pregnene-14alpha-ol-3,20-dione, delta4-pregnene-7beta,14alpha-diol-3,20-dione, and delta4-pregnene-6beta,11alpha-diol-3,20-dione by cell cultures of Lycopersicon esculentum. Cell cultures of Capsicum frutescens (green) metabolized progesterone to delta4-pregnene-20alpha-ol-3-one in very high yield, and Vinca rosea yielded delta4-pregnene-20beta-ol-3-one and delta4-pregnene-14alpha-ol-3,20-dione. A stereospecific reduction of the keto groups and a double bond and stereospecific introduction of hydroxyl groups at the 6, 11, and 14 positions have been observed. The mono- and dihydroxylated progesterones have not previously been reported as metabolic products of progesterone by plant cell systems and represent de novo hydroxylation of a nonglycosylated steroid. PMID:697360

Development of confocal laser microscope system for examination of microscopic characteristics of radiophotoluminescence glass dosemeters.

PubMed

Maki, Daisuke; Ishii, Tetsuya; Sato, Fuminobu; Kato, Yushi; Yamamoto, Takayoshi; Iida, Toshiyuki

2011-03-01

A confocal laser microscope system was developed for the measurement of radiophotoluminescence (RPL) photons emitted from a minute alpha-ray-irradiated area in an RPL glass dosemeter. The system was composed mainly of an inverted-type microscope, an ultraviolet laser, an XY movable stage and photon-counting circuits. The photon-counting circuits were effective in the reduction of the background noise level in the measurement of RPL photons. The performance of this microscope system was examined by the observation of standard RPL glass samples irradiated using (241)Am alpha rays. The spatial resolution of this system was ∼ 3 μm, and with regard to the sensitivity of this system, a hit of more than four to five alpha rays in unit area produced enough amount of RPL photons to construct the image.

Teaching Calculus with Wolfram|Alpha

ERIC Educational Resources Information Center

Dimiceli, Vincent E.; Lang, Andrew S. I. D.; Locke, LeighAnne

2010-01-01

This article describes the benefits and drawbacks of using Wolfram|Alpha as the platform for teaching calculus concepts in the lab setting. It is a result of our experiences designing and creating an entirely new set of labs using Wolfram|Alpha. We present the reasoning behind our transition from using a standard computer algebra system (CAS) to…

Communication requirements of sparse Cholesky factorization with nested dissection ordering

NASA Technical Reports Server (NTRS)

Naik, Vijay K.; Patrick, Merrell L.

1989-01-01

Load distribution schemes for minimizing the communication requirements of the Cholesky factorization of dense and sparse, symmetric, positive definite matrices on multiprocessor systems are presented. The total data traffic in factoring an n x n sparse symmetric positive definite matrix representing an n-vertex regular two-dimensional grid graph using n exp alpha, alpha not greater than 1, processors are shown to be O(n exp 1 + alpha/2). It is O(n), when n exp alpha, alpha not smaller than 1, processors are used. Under the conditions of uniform load distribution, these results are shown to be asymptotically optimal.

Sufficient and necessary condition of separability for generalized Werner states

DOE Office of Scientific and Technical Information (OSTI.GOV)

Deng Dongling; Chen Jingling

2009-02-15

In a celebrated paper [Optics Communications 179, 447, 2000], A.O. Pittenger and M.H. Rubin presented for the first time a sufficient and necessary condition of separability for the generalized Werner states. Inspired by their ideas, we generalized their method to a more general case. We obtain a sufficient and necessary condition for the separability of a specific class of N d-dimensional system (qudits) states, namely special generalized Werner state (SGWS): W{sup [d{sup N}]}(v)=(1-v)(I{sup (N)})/(d{sup N}) +v|{psi}{sub d}{sup N}><{psi}{sub d}{sup N}|, where |{psi}{sub d}{sup N}>={sigma}{sub i=0}{sup d-1}{alpha}{sub i}|i...i> is an entangled pure state of N qudits system and {alpha}{sub i} satisfiesmore » two restrictions: (i) {sigma}{sub i=0}{sup d-1}{alpha}{sub i}{alpha}{sub i}*=1; (ii) Matrix 1/d (I{sup (1)}+T{sigma}{sub i{ne}}{sub j}{alpha}{sub i}|i>

Perturbations of Jacobi polynomials and piecewise hypergeometric orthogonal systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Neretin, Yu A

2006-12-31

A family of non-complete orthogonal systems of functions on the ray [0,{infinity}] depending on three real parameters {alpha}, {beta}, {theta} is constructed. The elements of this system are piecewise hypergeometric functions with singularity at x=1. For {theta}=0 these functions vanish on [1,{infinity}) and the system is reduced to the Jacobi polynomials P{sub n}{sup {alpha}}{sup ,{beta}} on the interval [0,1]. In the general case the functions constructed can be regarded as an interpretation of the expressions P{sub n+{theta}}{sup {alpha}}{sup ,{beta}}. They are eigenfunctions of an exotic Sturm-Liouville boundary-value problem for the hypergeometric differential operator. The spectral measure for this problem ismore » found.« less

Alpha-cluster preformation factor within cluster-formation model for odd-A and odd-odd heavy nuclei

NASA Astrophysics Data System (ADS)

Saleh Ahmed, Saad M.

2017-06-01

The alpha-cluster probability that represents the preformation of alpha particle in alpha-decay nuclei was determined for high-intensity alpha-decay mode odd-A and odd-odd heavy nuclei, 82 < Z < 114, 111 < N < 174. This probability was calculated using the energy-dependent formula derived from the formulation of clusterisation states representation (CSR) and the hypothesised cluster-formation model (CFM) as in our previous work. Our previous successful determination of phenomenological values of alpha-cluster preformation factors for even-even nuclei motivated us to expand the work to cover other types of nuclei. The formation energy of interior alpha cluster needed to be derived for the different nuclear systems with considering the unpaired-nucleon effect. The results showed the phenomenological value of alpha preformation probability and reflected the unpaired nucleon effect and the magic and sub-magic effects in nuclei. These results and their analyses presented are very useful for future work concerning the calculation of the alpha decay constants and the progress of its theory.

Hubble Unveils Colorful and Turbulent Star-Birth Region on 100,000th Orbit Milestone

NASA Technical Reports Server (NTRS)

2008-01-01

[figure removed for brevity, see original site] Click on the image for orientation annotation

In commemoration of NASA's Hubble Space Telescope completing its 100,000th orbit in its 18th year of exploration and discovery, scientists at the Space Telescope Science Institute in Baltimore, Md., have aimed Hubble totake a snapshot of a dazzling region of celestial birth and renewal.

Hubble peered into a small portion of the nebula near the star cluster NGC 2074 (upper, left). The region is a firestorm of raw stellar creation, perhaps triggered by a nearby supernova explosion. It lies about 170,000 light-years away near the Tarantula nebula, one of the most active star-forming regions in our Local Group of galaxies.

The three-dimensional-looking image reveals dramatic ridges and valleys of dust, serpent-head 'pillars of creation,' and gaseous filaments glowing fiercely under torrential ultraviolet radiation. The region is on the edge of a dark molecular cloud that is an incubator for the birth of new stars.

The high-energy radiation blazing out from clusters of hot young stars already born in NGC 2074 is sculpting the wall of the nebula by slowly eroding it away. Another young cluster may be hidden beneath a circle of brilliant blue gas at center, bottom.

In this approximately 100-light-year-wide fantasy-like landscape, dark towers of dust rise above a glowing wall of gases on the surface of the molecular cloud. The seahorse-shaped pillar at lower, right is approximately 20 light-years long, roughly four times the distance between our Sun and the nearest star, Alpha Centauri.

The region is in the Large Magellanic Cloud (LMC), a satellite of our Milky Way galaxy. It is a fascinating laboratory for observing star-formation regions and their evolution. Dwarf galaxies like the LMC are considered to be the primitive building blocks of larger galaxies.

This representative color image was taken on August 10, 2008, with Hubble's Wide Field Planetary Camera 2. Red shows emission from sulfur atoms, green from glowing hydrogen, and blue from glowing oxygen.

Production of alpha-hydroxy carboxylic acids and esters from higher sugars using tandem catalyst systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Orazov, Marat; Davis, Mark E.

The present disclosure is directed to methods and composition used in the preparation of alpha-hydroxy carboxylic acids and esters from higher sugars using a tandem catalyst system comprising retro-aldol catalysts and Lewis acid catalysts. In some embodiments, these alpha-hydroxy carboxylic acids may be prepared from pentoses and hexoses. The retro-aldol and Lewis catalysts may be characterized by their respective ability to catalyze a 1,2-carbon shift reaction and a 1,2-hydride shift reaction on an aldose or ketose substrate.

Effects of a caffeine-free Cola nitida nuts extract on elastase/alpha-1-proteinase inhibitor balance.

PubMed

Daels-Rakotoarison, Dominique A; Kouakou, Gisèle; Gressier, Bernard; Dine, Thierry; Brunet, Claude; Luyckx, Michel; Bailleul, François; Trotin, Francis

2003-11-01

In an infection, polymorphonuclear neutrophils (PMN) become activated and they produce oxidizing compounds and elastase in the extracellular medium. Alpha-1-proteinase inhibitor (alpha1PI), a protease inhibitor which is inactivated by oxidants, is the main endogenous inhibitor of elastase helping to limit excessive elastase activity. This study evaluates the ability of a plant extract, Cola nitida nuts, to protect alpha1PI from inactivation by oxidizing compounds as reactive oxygen species. On the one hand, we have evaluated the direct effect of cola nut extract on neutrophil elastase, and on the H(2)O(2) and myeloperoxidase (MPO)-H(2)O(2) system via cell-free systems. Results showed that cola nut extract scavenges H(2)O(2) and therefore protects alpha1PI from HOCl which is produced from the MPO-H(2)O(2) system. Experiments also showed that cola extract has the capacity to limit elastase activity. On the other hand, we have worked on cellular systems including isolated PMN with the aim to study the effect of cola extract on PMN metabolism. PMN were stimulated with PMA, calcium ionophore or fMLP. Each stimulant possesses its own stimulation pathway. According to the inhibitory concentration obtained at 50%, the results on cellular systems led to the conclusion that cola extract can reduce elastase liberation from PMN. It can then be concluded that cola nut extract can protect alpha1PI from inactivation, and has an effect both on elastase liberation and elastase activity. The cola nut extract effect is rather biased towards a reduction in elastase release, thus limiting the injurious effects caused by this enzyme.

On Star-Planet Interaction: Magnetospheric Dynamics and Atmospheric Evolution

NASA Astrophysics Data System (ADS)

Tilley, Matthew Tilley

With the explosion of exoplanetary discoveries, the question of planetary habitability is at the forefront, and generates many interesting and complex questions. One of those questions: Are planetary global magnetic fields necessary for the development of complex surface organics and the development of life? Does a global field protect planetary atmospheres? What detection signatures can be gleaned from a planet or moon with a global field as opposed to one without? We have a wealth of in situ magnetospheric data from Earth, as well as solar system planets and their moons from several vital satellite missions, such as the Voyager missions, the Pioneer missions, Galileo, Cassini, Messenger, MAVEN, and New Horizons. Due to the distances involved, it is not tenable to send satellites to obtain data at exoplanetary bodies, so we rely on simulations and using solar system data as analog environments to help set ground truth validation for the numerical work. In this dissertation, I use a multifluid plasma model for gas giant magnetospheres to predict the potential dynamical consequences and detection signatures for giant exoplanets in a warm orbit (˜0.2 AU). I discuss the dynamics of plasma loss from an exomoon injected torus, and how the total mass flux out of the system is altered by increased stellar wind forcing as a function of orbital semi-major axis. Detection signatures for such a planet, including transit depth modifications due to plasma densities and radio emissions, show promise for further detecting and characterizing future systems. I also improve the multifluid model by implementing a full treatment of pressure anisotropy at Saturn, with a focus on the dynamics and structure of the magnetosphere. The improvements to the physics of the model generate more accurate system when compared to Cassini data; the anisotropic simulations show stronger current confinement of the Enceladus torus, consistent and well-structure flux interchange events, and global corotational convection that match more closely with the Cassini data than the isotropic model. Turning from giant planets to terrestrial, I use a coupled one-dimensional photochemical and radiative-convective climate model to investigate the effects of M dwarf stellar flare activity on an Earth-like atmosphere for an unmagnetized planet in the nominal habitable zone. I find that EM-only activity - even to the level of some of the most active stars yet observed - is insufficient at the age of the universe to reduce the ozone column to the point that UV-C radiation can reach the surface. However, repeated proton events from frequent daily flare activity, which has been observed on several M dwarfs, can erode the ozone column by several orders of magnitude, allowing the surface of the planet to be bathed in UV-C, which is sterilizing and detrimental to the development of complex organic structures. The ability of a strong planetary magnetic field to deflect incoming stellar wind and flare-energized protons seems to be of import to maintain surface habitability. I also use scaling laws to predict a potential atomic oxygen auroral signal from Proxima Centauri b, the detection of which would constrain the presence of an atmosphere and point to the presence of a magnetic field. The increased forcing from Proxima Centauri's stellar wind is expected to drive powerful emissions, orders of magnitude stronger than at Earth, and within easy reach of the next generation of observational telescopic instruments. Magnetic fields do seem to be important for both detection and potential shielding of the atmosphere of exoplanets, but much work remains to be performed. Future observations combined with simulations validated against solar system star-planet interactions will likely provide answers to these questions, and perhaps lead to a focus on specific planetary targets for extensive investigation of astrobiological interest.

The noradrenaline precursor L-threo-3,4-dihydroxyphenylserine exhibits antinociceptive activity via central alpha-adrenoceptors in the mouse.

PubMed Central

Kawabata, A.; Kasamatsu, K.; Umeda, N.; Takagi, H.

1994-01-01

1. Systemic (s.c. or p.o.) administration of L-threo-3,4-dihydroxyphenylserine (droxidopa, L-threo-DOPS; L-DOPS), a noradrenaline precursor, at a dose-range of 100-800 mg kg-1, produced naloxone-resistant antinociception in a dose-dependent manner in the mouse, as assessed by the tail flick test, kaolin-induced writhing test and formalin-induced nociception test. 2. Antinociception elicited by L-DOPS (400 mg kg-1, s.c.) was not affected by s.c. injection of benserazide, a peripherally preferential L-aromatic amino acid decarboxylase inhibitor, but was suppressed by its intracerebroventricular (i.c.v.) injection. 3. I.c.v. or intrathecal (i.t.) administration of the non-selective alpha-blocker, phentolamine, significantly reduced L-DOPS-induced antinociception. 4. I.c.v. administration of the alpha 1-blocker, prazosin, but not the alpha 2-blocker, yohimbine, abolished the antinociceptive effects of L-DOPS. In contrast, both blockers, when administered i.t., exhibited significant inhibitory effects. 5. These results suggest that systemic L-DOPS produces opioid-independent antinociception, mediated by supraspinal alpha 1-adrenoceptors and by spinal alpha 1- and alpha 2-adrenoceptors and may predict additional therapeutic applications of L-DOPS as an analgesic. PMID:7911717

Patterns of cortisol and alpha-amylase reactivity to psychosocial stress in maltreated women

PubMed Central

Mielock, Alyssa S.; Morris, Matthew C.; Rao, Uma

2016-01-01

Background Childhood maltreatment can trigger enduring changes in major stress response systems, particularly in the context of major depressive disorder (MDD). However, the relative impact of maltreatment versus MDD on hypothalamic-pituitary-adrenal axis and sympathetic-adrenal-medullary system stress reactivity is not well understood. Method This study examined salivary cortisol and alpha-amylase responses to the Trier Social Stress Test (TSST) in 26 maltreated (15 with current MDD) and 26 non-maltreated (17 with current MDD) women. Results Maltreated women showed greater anticipatory cortisol reactivity during the TSST protocol compared to non-maltreated women. Maltreated women also showed rapid deceleration in cortisol levels. Whereas non-maltreated women showed initial declines in alpha-amylase levels but rapidly increasing alpha-amylase levels during the TSST protocol, maltreated women did not exhibit changes in alpha-amylase levels during the TSST protocol. Contrary to expectation, MDD did not impact cortisol or alpha-amylase responses. Limitations The present study is limited by retrospective report of childhood maltreatment, cross-sectional design, and modest sample sizes. Conclusions These findings suggest that childhood maltreatment plays a greater role driving alterations in cortisol and alpha-amylase stress reactivity than MDD. Understanding the biological embedding of maltreatment is critical for elucidating mechanisms linking these experiences to risk for negative mental and physical health outcomes. PMID:27875756

A search for Lyman-alpha emission in beta Lyrae from Copernicus

NASA Technical Reports Server (NTRS)

Kondo, Y.; Mccluskey, G. E., Jr.

1974-01-01

High-resolution (0.2 A) spectrophotometric observations of the complex eclipsing binary beta Lyrae were obtained with the Princeton Telescope Spectrometer on the Copernicus satellite. We discuss the search for L-alpha emission in beta Lyrae and compare the Copernicus results with the OAO-2 observations of the same binary system. The possible L-alpha emission features observed from OAO-2 are identified as blends of the emission lines of other elements in the vicinity of L-alpha.

Antagonism of Lateral Amygdala Alpha1-Adrenergic Receptors Facilitates Fear Conditioning and Long-Term Potentiation

ERIC Educational Resources Information Center

Lazzaro, Stephanie C.; Hou, Mian; Cunha, Catarina; LeDoux, Joseph E.; Cain, Christopher K.

2010-01-01

Norepinephrine receptors have been studied in emotion, memory, and attention. However, the role of alpha1-adrenergic receptors in fear conditioning, a major model of emotional learning, is poorly understood. We examined the effect of terazosin, an alpha1-adrenergic receptor antagonist, on cued fear conditioning. Systemic or intra-lateral amygdala…

Alpha oscillations correlate with the successful inhibition of unattended stimuli.

PubMed

Händel, Barbara F; Haarmeier, Thomas; Jensen, Ole

2011-09-01

Because the human visual system is continually being bombarded with inputs, it is necessary to have effective mechanisms for filtering out irrelevant information. This is partly achieved by the allocation of attention, allowing the visual system to process relevant input while blocking out irrelevant input. What is the physiological substrate of attentional allocation? It has been proposed that alpha activity reflects functional inhibition. Here we asked if inhibition by alpha oscillations has behavioral consequences for suppressing the perception of unattended input. To this end, we investigated the influence of alpha activity on motion processing in two attentional conditions using magneto-encephalography. The visual stimuli used consisted of two random-dot kinematograms presented simultaneously to the left and right visual hemifields. Subjects were cued to covertly attend the left or right kinematogram. After 1.5 sec, a second cue tested whether subjects could report the direction of coherent motion in the attended (80%) or unattended hemifield (20%). Occipital alpha power was higher contralateral to the unattended side than to the attended side, thus suggesting inhibition of the unattended hemifield. Our key finding is that this alpha lateralization in the 20% invalidly cued trials did correlate with the perception of motion direction: Subjects with pronounced alpha lateralization were worse at detecting motion direction in the unattended hemifield. In contrast, lateralization did not correlate with visual discrimination in the attended visual hemifield. Our findings emphasize the suppressive nature of alpha oscillations and suggest that processing of inputs outside the field of attention is weakened by means of increased alpha activity.

Prestimulus neural oscillations inhibit visual perception via modulation of response gain.

PubMed

Chaumon, Maximilien; Busch, Niko A

2014-11-01

The ongoing state of the brain radically affects how it processes sensory information. How does this ongoing brain activity interact with the processing of external stimuli? Spontaneous oscillations in the alpha range are thought to inhibit sensory processing, but little is known about the psychophysical mechanisms of this inhibition. We recorded ongoing brain activity with EEG while human observers performed a visual detection task with stimuli of different contrast intensities. To move beyond qualitative description, we formally compared psychometric functions obtained under different levels of ongoing alpha power and evaluated the inhibitory effect of ongoing alpha oscillations in terms of contrast or response gain models. This procedure opens the way to understanding the actual functional mechanisms by which ongoing brain activity affects visual performance. We found that strong prestimulus occipital alpha oscillations-but not more anterior mu oscillations-reduce performance most strongly for stimuli of the highest intensities tested. This inhibitory effect is best explained by a divisive reduction of response gain. Ongoing occipital alpha oscillations thus reflect changes in the visual system's input/output transformation that are independent of the sensory input to the system. They selectively scale the system's response, rather than change its sensitivity to sensory information.

Involvement of central, but not placental corticotropin releasing hormone (CRH) in heat stress induced immunosuppression during pregnancy.

PubMed

Nakamura, H; Nagase, H; Ogino, K; Hatta, K; Matsuzaki, I

2001-03-01

To clarify whether corticotropin releasing hormone (CRH) and beta-endorphin (betaEP) system mediate maternal immunosuppression in pregnant rats exposed to heat through central or placental pathway, we examined the effects of intravenous (iv) (100 or 500 microg) or intracerebroventricular (icv) (5 microg) administration of CRH receptor antagonist alpha-helical CRH (9-41) on splenic natural killer cell activity (NKCA) as well as betaEP in blood, pituitary lobes, and placenta in pregnant rats at 15 to 16 days gestation. Two-way ANOVA revealed that heat reduced NKCA and elevated blood and pituitary betaEP but did not change placental betaEP. Iv administered 500 microg and icv administered alpha-helical CRH reversed the reduced NKCA and the elevated pituitary betaEP, while iv administration of 100 microg alpha-helical CRH did not. The increased blood betaEP was reversed by iv 100 and 500 microg alpha-helical CRH and icv administration. Both iv and icv administrations reduced placental betaEP independent of heat exposure. Thus, the response of placental betaEP to iv administration of alpha-helical CRH seemed to be stronger than that of pituitary betaEP. These results indicate that alpha-helical CRH which acts on pituitary betaEP antagonizes heat-induced immunosuppression during pregnancy, suggesting that immunosuppression produced by heat stress during pregnancy is mediated by the central CRH system. The placental CRH-betaEP system seems unlikely to be involved in the immunosuppression. Physiologic roles of placental CRH and opioid system should be clarified by future in vitro experiments using placenta specimen including placental immunocyte.

A new scanning system for alpha decay events as calibration sources for range-energy relation in nuclear emulsion

NASA Astrophysics Data System (ADS)

Yoshida, J.; Kinbara, S.; Mishina, A.; Nakazawa, K.; Soe, M. K.; Theint, A. M. M.; Tint, K. T.

2017-03-01

A new scanning system named "Vertex picker" has been developed to rapid collect alpha decay events, which are calibration sources for the range-energy relation in nuclear emulsion. A computer-controlled optical microscope scans emulsion layers exhaustively, and a high-speed and high-resolution camera takes their micrographs. A dedicated image processing picks out vertex-like shapes. Practical operations of alpha decay search were demonstrated by emulsion sheets of the KEK-PS E373 experiment. Alpha decays of nearly 28 events were detected in eye-check work on a PC monitor per hour. This yield is nearly 20 times more effective than that by the conventional eye-scan method. The speed and quality is acceptable for the coming new experiment, J-PARC E07.

Endothelial NOS is required for SDF-1alpha/CXCR4-mediated peripheral endothelial adhesion of c-kit+ bone marrow stem cells.

PubMed

Kaminski, Alexander; Ma, Nan; Donndorf, Peter; Lindenblatt, Nicole; Feldmeier, Gregor; Ong, Lee-Lee; Furlani, Dario; Skrabal, Christian A; Liebold, Andreas; Vollmar, Brigitte; Steinhoff, Gustav

2008-01-01

In the era of intravascular approaches for regenerative cell therapy, the underlying mechanisms of stem cell migration to non-marrow tissue have not been clarified. We hypothesized that next to a local inflammatory response implying adhesion molecule expression, endothelial nitric oxide synthase (eNOS)-dependent signaling is required for stromal- cell-derived factor-1 alpha (SDF-1alpha)-induced adhesion of c-kit+ cells to the vascular endothelium. SDF-1alpha/tumor necrosis factor-alpha (TNF-alpha)-induced c-kit+-cell shape change and migration capacity was studied in vitro using immunohistochemistry and Boyden chamber assays. In vivo interaction of c-kit+ cells from bone marrow with the endothelium in response to SDF-1alpha/TNF-alpha stimulation was visualized in the cremaster muscle microcirculation of wild-type (WT) and eNOS (-/-) mice using intravital fluorescence microscopy. In addition, NOS activity was inhibited with N-nitro-L-arginine-methylester-hydrochloride in WT mice. To reveal c-kit+-specific adhesion behavior, endogenous leukocytes (EL) and c-kit+ cells from peripheral blood served as control. Moreover, intercellular adhesion molecule-1 (ICAM-1) and CXCR4 were blocked systemically to determine their role in inflammation-related c-kit+-cell adhesion. In vitro, SDF-1alpha enhanced c-kit+-cell migration. In vivo, SDF-1alpha alone triggered endothelial rolling-not firm adherence-of c-kit+ cells in WT mice. While TNF-alpha alone had little effect on adhesion of c-kit+ cells, it induced maximum endothelial EL adherence. However, after combined treatment with SDF-1alpha+TNF-alpha, endothelial adhesion of c-kit+ cells increased independent of their origin, while EL adhesion was not further incremented. Systemic treatment with anti-ICAM-1 and anti-CXCR4-monoclonal antibody completely abolished endothelial c-kit+-cell adhesion. In N-nitro-L-arginine-methylester-hydrochloride-treated WT mice as well as in eNOS (-/-) mice, firm endothelial adhesion of c-kit+ cells was entirely abrogated, while EL adhesion was significantly increased. The chemokine SDF-1alpha mediates firm adhesion c-kit+ cells only in the presence of TNF-alpha stimulation via an ICAM-1- and CXCR4-dependent mechanism. The presence of eNOS appears to be a crucial and specific factor for firm c-kit+-cell adhesion to the vascular endothelium.

System for testing optical fibers

DOEpatents

Davies, Terence J.; Franks, Larry A.; Nelson, Melvin A.

1981-01-01

A system for nondestructively determining the attenuation coefficient, .alpha.(.lambda.), of low-loss optical fiber wave guides. Cerenkov light pulses are generated at a plurality of locations in the fiber by a beam of charged particles. The transit times of selected spectral components and their intensities are utilized to unfold the .alpha.(.lambda.) values over the measured spectrum.

AUTOMATED LITERATURE PROCESSING HANDLING AND ANALYSIS SYSTEM--FIRST GENERATION.

ERIC Educational Resources Information Center

Redstone Scientific Information Center, Redstone Arsenal, AL.

THE REPORT PRESENTS A SUMMARY OF THE DEVELOPMENT AND THE CHARACTERISTICS OF THE FIRST GENERATION OF THE AUTOMATED LITERATURE PROCESSING, HANDLING AND ANALYSIS (ALPHA-1) SYSTEM. DESCRIPTIONS OF THE COMPUTER TECHNOLOGY OF ALPHA-1 AND THE USE OF THIS AUTOMATED LIBRARY TECHNIQUE ARE PRESENTED. EACH OF THE SUBSYSTEMS AND MODULES NOW IN OPERATION ARE…

Effect of alpha-tocopherol on lipid peroxidation and antioxidant system in fibrosarcoma bearing rats.

PubMed

Vasavi, H; Thangaraju, M; Sachdanandam, P

1994-02-23

The anticarcinogenic activity of alpha-tocopherol (Vitamin E) was tried in fibrosarcoma induced rats through its antioxidative potential. The rate of formation of malondialdehyde (MDA), the end product of lipid peroxidation was analysed in alpha-tocopherol (400 mg/kg body weight) treated and untreated fibrosarcoma bearing rats with respective controls. The levels of non-enzymic antioxidants like, glutathione and vitamin E, and enzymic antioxidants viz., catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and glutathione-S-transferase (GST) were assayed as well. Significantly increased (p < 0.001) level of lipid peroxide was observed with concomitant decreases in the level of enzymic and non-enzymic antioxidants in fibrosarcoma bearing rats when compared with control animals. In alpha-tocopherol supplemented animals, the corrected level of these parameters were observed likely to near normal values. Thus, alpha-tocopherol can be accepted to pose first line of defense mechanism against excessively formed reactive species due to impaired antioxidant systems in fibrosarcoma conditions, that cause membrane damage leading to deleterious effects.

The self-consistent calculation of pseudo-molecule energy levels, construction of energy level correlation diagrams and an automated computation system for SCF-X(Alpha)-SW calculations

NASA Technical Reports Server (NTRS)

Schlosser, H.

1981-01-01

The self consistent calculation of the electronic energy levels of noble gas pseudomolecules formed when a metal surface is bombarded by noble gas ions is discussed along with the construction of energy level correlation diagrams as a function of interatomic spacing. The self consistent field x alpha scattered wave (SCF-Xalpha-SW) method is utilized. Preliminary results on the Ne-Mg system are given. An interactive x alpha programming system, implemented on the LeRC IBM 370 computer, is described in detail. This automated system makes use of special PROCDEFS (procedure definitions) to minimize the data to be entered manually at a remote terminal. Listings of the special PROCDEFS and of typical input data are given.

Ebselen: A thioredoxin reductase-dependent catalyst for {alpha}-tocopherol quinone reduction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fang Jianguo; Zhong Liangwei; Zhao Rong

2005-09-01

The thioredoxin system, composed of thioredoxin (Trx), thioredoxin reductase (TrxR), and NADPH, is a powerful protein disulfide reductase system with a broad substrate specificity. Recently the selenazol drug ebselen was shown to be a substrate for both mammalian TrxR and Trx. We examined if {alpha}-tocopherol quinone (TQ), a product of {alpha}-tocopherol oxidation, is reduced by ebselen in the presence of TrxR, since TQ was not a substrate for the enzyme itself. Ebselen reduction of TQ in the presence of TrxR was caused by ebselen selenol, generated from fast reduction of ebselen by the enzyme. TQ has no intrinsic antioxidant activity,more » while the product of reduction of TQ, {alpha}-tocopherolhydroquinone (TQH{sub 2}), is a potent antioxidant. The thioredoxin system dependence of ebselen to catalyze reduction of other oxidized species, such as hydrogen peroxide, dehydroascorbate, and peroxynitrite, is discussed. The ability of ebselen to reduce TQ via the thioredoxin system is a novel mechanism to explain the effects of the drug as an antioxidant in vivo.« less

Liposomal formulation of {alpha}-tocopheryl maleamide: In vitro and in vivo toxicological profile and anticancer effect against spontaneous breast carcinomas in mice

DOE Office of Scientific and Technical Information (OSTI.GOV)

Turanek, Jaroslav; Wang Xiufang; Knoetigova, Pavlina

2009-06-15

The vitamin E analogue {alpha}-tocopheryl succinate ({alpha}-TOS) is an efficient anti-cancer drug. Improved efficacy was achieved through the synthesis of {alpha}-tocopheryl maleamide ({alpha}-TAM), an esterase-resistant analogue of {alpha}-tocopheryl maleate. In vitro tests demonstrated significantly higher cytotoxicity of {alpha}-TAM towards cancer cells (MCF-7, B16F10) compared to {alpha}-TOS and other analogues prone to esterase-catalyzed hydrolysis. However, in vitro models demonstrated that {alpha}-TAM was cytotoxic to non-malignant cells (e.g. lymphocytes and bone marrow progenitors). Thus we developed lyophilized liposomal formulations of both {alpha}-TOS and {alpha}-TAM to solve the problem with cytotoxicity of free {alpha}-TAM (neurotoxicity and anaphylaxis), as well as the low solubilitymore » of both drugs. Remarkably, neither acute toxicity nor immunotoxicity implicated by in vitro tests was detected in vivo after application of liposomal {alpha}-TAM, which significantly reduced the growth of cancer cells in hollow fiber implants. Moreover, liposomal formulation of {alpha}-TAM and {alpha}-TOS each prevented the growth of tumours in transgenic FVB/N c-neu mice bearing spontaneous breast carcinomas. Liposomal formulation of {alpha}-TAM demonstrated anti-cancer activity at levels 10-fold lower than those of {alpha}-TOS. Thus, the liposomal formulation of {alpha}-TAM preserved its strong anti-cancer efficacy while eliminating the in vivo toxicity found of the free drug applied in DMSO. Liposome-based targeted delivery systems for analogues of vitamin E are of interest for further development of efficient and safe drug formulations for clinical trials.« less

Development of Flight Slit-Jaw Optics for Chromospheric Lyman-Alpha SpectroPolarimeter

NASA Technical Reports Server (NTRS)

Kubo, Masahito; Suematsu, Yoshinori; Kano, Ryohei; Bando, Takamasa; Hara, Hirohisa; Narukage, Noriyuki; Katsukawa, Yukio; Ishikawa, Ryoko; Ishikawa, Shin-nosuke; Kobiki, Toshihiko;

Hepatocyte nuclear factor-4alpha is a central transactivator of the mouse Ntcp gene.

PubMed

Geier, Andreas; Martin, Ina V; Dietrich, Christoph G; Balasubramaniyan, Natarajan; Strauch, Sonja; Suchy, Frederick J; Gartung, Carsten; Trautwein, Christian; Ananthanarayanan, Meenakshisundaram

2008-08-01

Sodium taurocholate cotransporting polypeptide (Ntcp) is the major uptake system for conjugated bile acids. Deletions of hepatocyte nuclear factor (HNF)-1alpha and retinoid X receptor-alpha:retinoic acid receptor-alpha binding sites in the mouse 5'-flanking region corresponding to putatively central regulatory elements of rat Ntcp do not significantly reduce promoter activity. We hypothesized that HNF-4alpha, which is increasingly recognized as a central regulator of hepatocyte function, may directly transactivate mouse (mNtcp). A 1.1-kb 5'-upstream region including the mouse Ntcp promoter was cloned and compared with the rat promoter. In contrast to a moderate 3.5-fold activation of mNtcp by HNF-1alpha, HNF-4alpha cotransfection led to a robust 20-fold activation. Deletion analysis of mouse and rat Ntcp promoters mapped a conserved HNF-4alpha consensus site at -345/-326 and -335/-316 bp, respectively. p-475bpmNtcpLUC is not transactivated by HNF-1alpha but shows a 50-fold enhanced activity upon cotransfection with HNF-4alpha. Gel mobility shift assays demonstrated a complex of the HNF-4alpha-element formed with liver nuclear extracts that was blocked by an HNF-4alpha specific antibody. HNF-4alpha binding was confirmed by chromatin immunoprecipitation. Using Hepa 1-6 cells, HNF-4alpha-knockdown resulted in a significant 95% reduction in NTCP mRNA. In conclusion, mouse Ntcp is regulated by HNF-4alpha via a conserved distal cis-element independently of HNF-1alpha.

Transmembrane Na+ and Ca2+ electrochemical gradients in cardiac muscle and their relationship to force development

PubMed Central

1982-01-01

Na+- and CA2+-sensitive microelectrodes were used to measure intracellular Na+ and Ca2+ activities (alpha iCa) of sheep ventricular muscle and Purkinje strands to study the interrelationship between Na+ and Ca2+ electrochemical gradients (delta muNa and delta muCa) under various conditions. In ventricular muscle, alpha iNa was 6.4 +/- 1.2 mM and alpha iCa was 87 +/- 20 nM ([Ca/+] = 272 nM). A graded decrease of external Na+ activity (alpha oNa) resulted in decrease of alpha iNa, and increase of alpha iCa. There was increase of twitch tension in low- alpha oNa solutions, and occasional increase of resting tension in 40% alpha oNa. Increase of external Ca2+ (alpha oCa) resulted in increase of alpha iCa and decrease of alpha iNa. Decrease of alpha oCa resulted in decrease of alpha iCa and increase of alpha iNa. The apparent resting Na-Ca energy ratio (delta muCa/delta muNa) was between 2.43 and 2.63. When the membrane potential (Vm) was depolarized by 50 mM K+ in ventricular muscle, Vm depolarized by 50 mV, alpha iNa decreased, and alpha iCa increased, with the development of a contracture. The apparent energy coupling ratio did not change with depolarization. 5 x 10(-6) M ouabain induced a large increase in alpha iNa ad alpha iCa, accompanied by an increase in twitch and resting tension. Under the conditions we have studied, delta muNa and delta muCa appeared to be coupled and n was nearly constant at 2.5, as would be expected if the Na-Ca exchange system was able to set the steady level of alpha iCa. Tension threshold was about 230 nM alpha iCa. The magnitude of twitch tension was directly related to alpha iCa. PMID:6292328

Functional relationships among monitoring performance: Subjective report of thought process and compromising states of awareness

NASA Technical Reports Server (NTRS)

Freeman, Frederick

1995-01-01

A biocybernetic system for use in adaptive automation was evaluated using EEG indices based on the beta, alpha, and theta bandwidths. Subjects performed a compensatory tracking task while their EEG was recorded and one of three engagement indices was derived: beta/(alpha + theta), beta/alpha, or 1/alpha. The task was switched between manual and automatic modes as a function of the subjects' level of engagement and whether they were under a positive or negative feedback condition. It was hypothesized that negative feedback would produce more switches between manual and automatic modes, and that the beta/(alpha + theta) index would produce the strongest effect. The results confirmed these hypotheses. There were no systematic changes in these effects over three 16-minute trials. Tracking performance was found to be better under negative feedback. An analysis of the different EEG bands under positive and negative feedback in manual and automatic modes found more beta power in the positive feedback/manual condition and less in the positive feedback/automatic condition. The opposite effect was observed for alpha and theta power. The implications of biocybernetic systems for adaptive automation are discussed.

Pesticide exposure exacerbates alpha-synucleinopathy in an A53T transgenic mouse model.

PubMed

Norris, Erin H; Uryu, Kunihiro; Leight, Susan; Giasson, Benoit I; Trojanowski, John Q; Lee, Virginia M-Y

2007-02-01

The factors initiating or contributing to the pathogenesis of Parkinson's disease and related neurodegenerative synucleinopathies are still largely unclear, but environmental factors such as pesticides have been implicated. In this study, A53T mutant human alpha-synuclein transgenic mice (M83), which develop alpha-synuclein neuropathology, were treated with the pesticides paraquat and maneb (either singly or together), and their effects were analyzed. Immunohistochemical and biochemical analyses showed that chronic treatment of M83 transgenic mice with both pesticides (but not with either pesticide alone) drastically increased neuronal alpha-synuclein pathology throughout the central nervous system including the hippocampus, cerebellum, and sensory and auditory cortices. alpha-Synuclein-associated mitochondrial degeneration was observed in M83 but not in wild-type alpha-synuclein transgenic mice. Because alpha-synuclein inclusions accumulated in pesticide-exposed M83 transgenic mice without a motor phenotype, we conclude that alpha-synuclein aggregate formation precedes disease onset. These studies support the notion that environmental factors causing nitrative damage are closely linked to mechanisms underlying the formation of alpha-synuclein pathologies and the onset of Parkinson's-like neurodegeneration.

A spectroscopic search for colliding stellar winds in O-type close binary systems. II - Plaskett's star (HD 47129)

NASA Technical Reports Server (NTRS)

Wiggs, Michael S.; Gies, Douglas R.

1992-01-01

New evidence for colliding winds in the massive O-type binary system Plaskett's star is reported. High S/N ratio spectra of the H-alpha and He I 6678 emission lines are presented, and their orbital phase-related variations are examined in order to derive the locations and motions of the high-density gas in the system. Radial velocity cures for several absorption and emission lines associated with the photosphere of the primary are also provided. The H-alpha emission profiles are complex, with very broad wings and a sharp spikelike feature that approximately follows the motion of the primary star. The radial velocity curve for this spike lags behind the photospheric velocity curve of the primary by 0.066 in phase. It is suggested that the high-velocity H-alpha emission is related to instabilities in the intershock region between the two component stars. The H-alpha phase-related variations are compared with those observed in the UV wind lines in IUE archival spectra.

HIGH RESOLUTION H{alpha} IMAGES OF THE BINARY LOW-MASS PROPLYD LV 1 WITH THE MAGELLAN AO SYSTEM

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu, Y.-L.; Close, L. M.; Males, J. R.

2013-09-01

We utilize the new Magellan adaptive optics system (MagAO) to image the binary proplyd LV 1 in the Orion Trapezium at H{alpha}. This is among the first AO results in visible wavelengths. The H{alpha} image clearly shows the ionization fronts, the interproplyd shell, and the cometary tails. Our astrometric measurements find no significant relative motion between components over {approx}18 yr, implying that LV 1 is a low-mass system. We also analyze Large Binocular Telescope AO observations, and find a point source which may be the embedded protostar's photosphere in the continuum. Converting the H magnitudes to mass, we show thatmore » the LV 1 binary may consist of one very-low-mass star with a likely brown dwarf secondary, or even plausibly a double brown dwarf. Finally, the magnetopause of the minor proplyd is estimated to have a radius of 110 AU, consistent with the location of the bow shock seen in H{alpha}.« less

Sociodemographic Risk, Parenting, and Effortful Control: Relations to Salivary Alpha-amylase and Cortisol in Early Childhood

PubMed Central

Taylor, Zoe E.; Spinrad, Tracy L.; VanSchyndel, Sarah K.; Eisenberg, Nancy; Huynh, Jacqueline; Sulik, Michael J.; Granger, Douglas A.

2012-01-01

Early sociodemographic risk, parenting, and temperament were examined as predictors of the activity of children’s (N = 148; 81 boys, 67 girls) hypothalamic-pituitary-adrenal axis and autonomic nervous system. Demographic risk was assessed at 18 months (T1), intrusive-overcontrolling parenting and effortful control were assessed at 30 months (T2), and salivary cortisol and alpha-amylase were collected at 72 (T3) months of age. Demographic risk at T1 predicted lower levels of children’s effortful control and higher levels of mothers’ intrusive-overcontrolling parenting at T2. Intrusive-overcontrolling parenting at T2 predicted higher levels of children’s cortisol and alpha-amylase at T3, but effortful control did not uniquely predict children’s cortisol or alpha-amylase. Findings support the open nature of stress responsive physiological systems to influence by features of the early caregiving environment and underscore the utility of including measures of these systems in prevention trials designed to influence child outcomes by modifying parenting behavior. PMID:22949301

Amplitude modulation of alpha-band rhythm caused by mimic collision: MEG study.

PubMed

Yokosawa, Koichi; Watanabe, Tatsuya; Kikuzawa, Daichi; Aoyama, Gakuto; Takahashi, Makoto; Kuriki, Shinya

2013-01-01

Detection of a collision risk and avoiding the collision are important for survival. We have been investigating neural responses when humans anticipate a collision or intend to take evasive action by applying collision-simulating images in a predictable manner. Collision-simulating images and control images were presented in random order to 9 healthy male volunteers. A cue signal was also given visually two seconds before each stimulus to enable each participant to anticipate the upcoming stimulus. Magnetoencephalograms (MEG) were recorded with a 76-ch helmet system. The amplitude of alpha band (8-13 Hz) rhythm when anticipating the upcoming collision-simulating image was significantly smaller than that when anticipating control images even just after the cue signal. This result demonstrates that anticipating a negative (dangerous) event induced event-related desynchronization (ERD) of alpha band activity, probably caused by attention. The results suggest the feasibility of detecting endogenous brain activities by monitoring alpha band rhythm and its possible applications to engineering systems, such as an automatic collision evasion system for automobiles.

A phoswich detector for simultaneous alpha-gamma spectroscopy

NASA Astrophysics Data System (ADS)

Moghadam, S. Rajabi; Feghhi, S. A. H.; Safari, M. J.

2015-11-01

Phoswich detectors are of value for radiation spectroscopy, especially in cases where a low-cost solution for a mixed radiation field is desired. Meanwhile, simultaneous spectroscopy of alpha particles and gamma-rays has many applications in quantification and distinguishing the alpha-emitting radionuclides which usually occur in the analysis of environmental solid samples. Here, we have developed a system for detection of radioactive actinides (e.g., 241Am) based on the alpha-gamma coincidence technique. The underlying concept, is to assemble two appropriately selected scintillators (i.e., a fast and a slow one) together with a discriminating unit for analysis of their data. Detailed Monte Carlo simulation procedure has been developed using the GEANT4 toolkit to design and find enough knowledge about the response of the system in the studied radiation field. Various comparisons were made between experimental and simulation data which showed appropriate agreement between them. The calibration was performed and the MDA was estimated as 60 mBq for the phoswich system.

Development of a bioassay to screen for chemicals mimicking the anti-aging effects of calorie restriction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chiba, Takuya, E-mail: takuya@nagasaki-u.ac.jp; Tsuchiya, Tomoshi; Komatsu, Toshimitsu

2010-10-15

Research highlights: {yields} We identified four sequence motifs lying upstream of putative pro-longevity genes. {yields} One of these motifs binds to HNF-4{alpha}. {yields} HNF-4{alpha}/PGC-1{alpha} could up-regulate the transcription of a reporter gene linked to this motif. {yields} The reporter system described here could be used to screen candidate anti-aging molecules. -- Abstract: Suppression of the growth hormone/insulin-like growth factor-I pathway in Ames dwarf (DF) mice, and caloric restriction (CR) in normal mice extends lifespan and delays the onset of age-related disorders. In combination, these interventions have an additive effect on lifespan in Ames DF mice. Therefore, common signaling pathways regulatedmore » by DF and CR could have additive effects on longevity. In this study, we tried to identity the signaling mechanism and develop a system to assess pro-longevity status in cells and mice. We previously identified genes up-regulated in the liver of DF and CR mice by DNA microarray analysis. Motif analysis of the upstream sequences of those genes revealed four major consensus sequence motifs, which have been named dwarfism and calorie restriction-responsive elements (DFCR-REs). One of the synthesized sequences bound to hepatocyte nuclear factor-4{alpha} (HNF-4{alpha}), an important transcription factor involved in liver metabolism. Furthermore, using this sequence information, we developed a highly sensitive bioassay to identify chemicals mimicking the anti-aging effects of CR. When the reporter construct, containing an element upstream of a secreted alkaline phosphatase (SEAP) gene, was co-transfected with HNF-4{alpha} and its regulator peroxisome proliferator-activated receptor (PPAR) {gamma} coactivator-1{alpha} (PGC-1{alpha}), SEAP activity was increased compared with untransfected controls. Moreover, transient transgenic mice established using this construct showed increased SEAP activity in CR mice compared with ad libitum-fed mice. These data suggest that because of its rapidity, ease of use, and specificity, our bioassay will be more useful than the systems currently employed to screen for CR mimetics, which mimic the beneficial effects of CR. Our system will be particularly useful for high-throughput screening of natural and synthetic candidate molecules.« less

5Alpha-Reduced Steroids Are Major Metabolites in the Early Equine Embryo Proper and Its Membranes.

PubMed

Raeside, James I; Christie, Heather L; Betteridge, Keith J

2015-09-01

Steroid production and metabolism by early conceptuses are very important for the establishment and maintenance of pregnancy in horses. Our earlier work suggested the possible formation of 5alpha-reduced steroids in equine conceptuses. We have now demonstrated the formation of 5alpha-reduced metabolites of androstenedione, testosterone, and progesterone by the embryo and its membranes. A total of 44 conceptuses were collected from 26 mares between 20 and 31 days of pregnancy. Tissues from the embryo proper and from the separated components of the conceptus (bilaminar and trilaminar trophoblast, allantois) were incubated with tritium-labeled substrates. 5Alpha-reduced metabolites (5alpha-dihydro- and 3beta,5alpha-tetrahydro- steroids) as radiolabeled products were identified from a series of chromatographic steps using four solvent systems for high-performance liquid chromatography. Use of a 5alpha-reductase inhibitor confirmed the metabolites were indeed 5alpha-reduced steroids. For the embryo, the only products from androstenedione were 5alpha-dihydroandrostenedione and 3beta,5alpha-tetrahydroandrostenedione, with no evidence of more polar metabolites; there was some 3beta,5alpha-tetrahydrotestosterone but no 5alpha-dihydrotestosterone from testosterone, and formation of androstenedione was followed by the production of 5alpha-dihydroandrostenedione and 3beta,5alpha-tetrahydroandrostenedione. The major 5alpha-reduced product from progesterone was 3beta,5alpha-tetrahydroprogesterone, with lesser amounts of 5alpha-dihydroprogesterone. For the membranes, reductions to tetrahydro, 5alpha-reduced steroids were prominent in most instances, but also present were considerable amounts of products more polar than the substrates. The well-recognized activity of some 5alpha-reduced steroids--for example, 5alpha-dihydrotestosterone in male sexual differentiation--provokes interest in their even earlier appearance, as seen in this study, and suggests a possible role for them in early embryonic development in horses and, more generally, in other species. © 2015 by the Society for the Study of Reproduction, Inc.

Radiocurability by Targeting Tumor Necrosis Factor-{alpha} Using a Bispecific Antibody in Carcinoembryonic Antigen Transgenic Mice

DOE Office of Scientific and Technical Information (OSTI.GOV)

Larbouret, Christel; Robert, Bruno; Linard, Christine

2007-11-15

Purpose: Tumor necrosis factor-{alpha} (TNF-{alpha}) enhances radiotherapy (RT) killing of tumor cells in vitro and in vivo. To overcome systemic side effects, we used a bispecific antibody (BsAb) directed against carcinoembryonic antigen (CEA) and TNF-{alpha} to target this cytokine in a CEA-expressing colon carcinoma. We report the evaluation of this strategy in immunocompetent CEA-transgenic mice. Methods and Materials: The murine CEA-transfected colon carcinoma MC-38 was used for all experiments. In vitro, clonogenic assays were performed after RT alone, TNF-{alpha} alone, and RT plus TNF-{alpha}. In vivo, the mice were randomly assigned to treatment groups: control, TNF-{alpha}, BsAb, BsAb plus TNF-{alpha},more » RT, RT plus TNF-{alpha}, and RT plus BsAb plus TNF-{alpha}. Measurements of endogenous TNF-{alpha} mRNA levels and evaluation of necrosis (histologic evaluation) were assessed per treatment group. Results: In vitro, combined RT plus TNF-{alpha} resulted in a significant decrease in the survival fraction at 2 Gy compared with RT alone (p < 0.00001). In vivo, we observed a complete response in 5 (50%) of 10, 2 (20%) of 10, 2 (18.2%) of 11, and 0 (0%) of 12 treated mice in the RT plus BsAb plus TNF-{alpha}, RT plus TNF-{alpha}, RT alone, and control groups, respectively. This difference was statistically significant when TNF-{alpha} was targeted with the BsAb (p = 0.03). The addition of exogenous TNF-{alpha} to RT significantly increased the endogenous TNF-{alpha} mRNA level, particularly when TNF-{alpha} was targeted with BsAb (p < 0.01). The percentages of necrotic area were significantly augmented in the RT plus BsAb plus TNF-{alpha} group. Conclusion: These results suggest that targeting TNF-{alpha} with the BsAb provokes RT curability in a CEA-expressing digestive tumor syngenic model and could be considered as a solid rationale for clinical trials.« less

Haemolytic anaemia to the alpha-interferon treatment: a proposed mechanism.

PubMed

Barbolla, L; Paniagua, C; Outeiriño, J; Prieto, E; Sánchez Fayos, J

1993-01-01

Auto-immune haemolytic anaemia (AIHA) has been found in a case of alpha-interferon treatment. Serum antibody and eluate were positive in the absence of the drug. Although the patient recovered after the treatment was stopped, DAGT remained positive for at least 8 months. The mechanism proposed to explain why this drug induced AIHA is similar to that proposed for alpha-methyl-dopa. Drugs could alter the red cell membrane and impair the immune system. Such changes have been observed with alpha-interferon and were related with increased autoimmunity.

Serotonergic neurotoxic metabolites of ecstasy identified in rat brain.

PubMed

Jones, Douglas C; Duvauchelle, Christine; Ikegami, Aiko; Olsen, Christopher M; Lau, Serrine S; de la Torre, Rafael; Monks, Terrence J

2005-04-01

The selective serotonergic neurotoxicity of 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) depends on their systemic metabolism. We have recently shown that inhibition of brain endothelial cell gamma-glutamyl transpeptidase (gamma-GT) potentiates the neurotoxicity of both MDMA and MDA, indicating that metabolites that are substrates for this enzyme contribute to the neurotoxicity. Consistent with this view, glutathione (GSH) and N-acetylcysteine conjugates of alpha-methyl dopamine (alpha-MeDA) are selective neurotoxicants. However, neurotoxic metabolites of MDMA or MDA have yet to be identified in brain. Using in vivo microdialysis coupled to liquid chromatography-tandem mass spectroscopy and a high-performance liquid chromatography-coulometric electrode array system, we now show that GSH and N-acetylcysteine conjugates of N-methyl-alpha-MeDA are present in the striatum of rats administered MDMA by subcutaneous injection. Moreover, inhibition of gamma-GT with acivicin increases the concentration of GSH and N-acetylcysteine conjugates of N-methyl-alpha-MeDA in brain dialysate, and there is a direct correlation between the concentrations of metabolites in dialysate and the extent of neurotoxicity, measured by decreases in serotonin (5-HT) and 5-hydroxyindole acetic (5-HIAA) levels. Importantly, the effects of acivicin are independent of MDMA-induced hyperthermia, since acivicin-mediated potentiation of MDMA neurotoxicity occurs in the context of acivicin-mediated decreases in body temperature. Finally, we have synthesized 5-(N-acetylcystein-S-yl)-N-methyl-alpha-MeDA and established that it is a relatively potent serotonergic neurotoxicant. Together, the data support the contention that MDMA-mediated serotonergic neurotoxicity is mediated by the systemic formation of GSH and N-acetylcysteine conjugates of N-methyl-alpha-MeDA (and alpha-MeDA). The mechanisms by which such metabolites access the brain and produce selective serotonergic neurotoxicity remain to be determined.

Comparative antioxidant activity of tocotrienols and other natural lipid-soluble antioxidants in a homogeneous system, and in rat and human lipoproteins.

PubMed

Suarna, C; Hood, R L; Dean, R T; Stocker, R

1993-02-24

The antioxidant activity of tocotrienols toward peroxyl radicals was compared with that of other natural lipid-soluble antioxidants in three different systems by measuring the temporal disappearance of antioxidants and the formation of lipid hydroperoxides. In homogeneous solution, the initial rates of consumption of the various antioxidants, assessed by competition experiments between pairs of antioxidants for radicals, decreased in the order: ubiquinol-10 approximately ubiquinol-9 > alpha-tocopherol approximately alpha-tocotrienol > beta-carotene approximately lycopene > gamma-tocopherol approximately gamma-tocotrienol. Following in vitro incubation of human plasma with alpha-tocotrienol, this form of vitamin E was present in all classes of lipoproteins isolated from the supplemented plasma. Dietary supplementation of rats and humans with a tocotrienol-rich preparation resulted in a dose-dependent appearance of alpha- and gamma-tocotrienols in plasma and all circulating lipoproteins, respectively. Exposure of such enriched rat plasma to aqueous peroxyl radicals resulted in simultaneous consumption of the alpha- and then gamma-isomers of vitamin E. The sequence of radical-induced consumption of antioxidants in freshly isolated, in vitro and in vivo tocotrienol-enriched low density lipoprotein (LDL) was again ubiquinol-10 > alpha-tocotrienol approximately alpha-tocopherol > carotenoids > gamma-tocopherol approximately gamma-tocotrienol. Under conditions where radicals were generated at constant rates, the rate of lipid hydroperoxide formation in LDL was not constant. It proceeded in at least three stages separated by the phase of ubiquinol-10 consumption and, subsequently, that of alpha-tocopherol/alpha-tocotrienol. Our results show that dietary tocotrienols become incorporated into circulating human lipoproteins where they react with peroxyl radicals as efficiently as the corresponding tocopherol isomers.

Patterns of cortisol and alpha-amylase reactivity to psychosocial stress in maltreated women.

PubMed

Mielock, Alyssa S; Morris, Matthew C; Rao, Uma

2017-02-01

Childhood maltreatment can trigger enduring changes in major stress response systems, particularly in the context of major depressive disorder (MDD). However, the relative impact of maltreatment versus MDD on hypothalamic-pituitary-adrenal axis and sympathetic-adrenal-medullary system stress reactivity is not well understood. This study examined salivary cortisol and alpha-amylase responses to the Trier Social Stress Test (TSST) in 26 maltreated (15 with current MDD) and 26 non-maltreated (17 with current MDD) women. Maltreated women showed greater anticipatory cortisol reactivity during the TSST protocol compared to non-maltreated women. Maltreated women also showed rapid deceleration in cortisol levels. Whereas non-maltreated women showed initial declines in alpha-amylase levels but rapidly increasing alpha-amylase levels during the TSST protocol, maltreated women did not exhibit changes in alpha-amylase levels during the TSST protocol. Contrary to expectation, MDD did not impact cortisol or alpha-amylase responses. The present study is limited by retrospective report of childhood maltreatment, cross-sectional design, and modest sample sizes. These findings suggest that childhood maltreatment plays a greater role driving alterations in cortisol and alpha-amylase stress reactivity than MDD. Understanding the biological embedding of maltreatment is critical for elucidating mechanisms linking these experiences to risk for negative mental and physical health outcomes. Copyright © 2016 Elsevier B.V. All rights reserved.

The possible participation of esters as well as amides in prebiotic polymers.

NASA Technical Reports Server (NTRS)

Rich, A.

1971-01-01

Demonstration that alpha-hydroxy acids may have participated in the formation of prebiological polymers in a manner similar to the participation of alpha-amino acids. Ex periments are described which indicate that the system for forming peptide bonds in present-day biological organisms is equally competent in forming ester and polyester bonds. In particular, the experiments described are directed toward answering questions regarding the action of peptidyl transferase in ester formation. Also, an attempt is made to determine whether a complete protein synthetic system can operate with transfer RNA molecules which have alpha-hydroxyl acids attached to them instead of alpha-amino acids, using both synthetic and natural mRNA. The ability of ribosomal peptidyl transferase to catalyze the formation of an ester bond as well as its normal product, the peptide bond, is demonstrated.

Space Station alpha joint bearing

NASA Technical Reports Server (NTRS)

Everman, Michael R.; Jones, P. Alan; Spencer, Porter A.

1987-01-01

Perhaps the most critical structural system aboard the Space Station is the Solar Alpha Rotary Joint which helps align the power generation system with the sun. The joint must provide structural support and controlled rotation to the outboard transverse booms as well as power and data transfer across the joint. The Solar Alpha Rotary Joint is composed of two transition sections and an integral, large diameter bearing. Alpha joint bearing design presents a particularly interesting problem because of its large size and need for high reliability, stiffness, and on orbit maintability. The discrete roller bearing developed is a novel refinement to cam follower technology. It offers thermal compensation and ease of on-orbit maintenance that are not found in conventional rolling element bearings. How the bearing design evolved is summarized. Driving requirements are reviewed, alternative concepts assessed, and the selected design is described.

FFT transformed quantitative EEG analysis of short term memory load.

PubMed

Singh, Yogesh; Singh, Jayvardhan; Sharma, Ratna; Talwar, Anjana

2015-07-01

The EEG is considered as building block of functional signaling in the brain. The role of EEG oscillations in human information processing has been intensively investigated. To study the quantitative EEG correlates of short term memory load as assessed through Sternberg memory test. The study was conducted on 34 healthy male student volunteers. The intervention consisted of Sternberg memory test, which runs on a version of the Sternberg memory scanning paradigm software on a computer. Electroencephalography (EEG) was recorded from 19 scalp locations according to 10-20 international system of electrode placement. EEG signals were analyzed offline. To overcome the problems of fixed band system, individual alpha frequency (IAF) based frequency band selection method was adopted. The outcome measures were FFT transformed absolute powers in the six bands at 19 electrode positions. Sternberg memory test served as model of short term memory load. Correlation analysis of EEG during memory task was reflected as decreased absolute power in Upper alpha band in nearly all the electrode positions; increased power in Theta band at Fronto-Temporal region and Lower 1 alpha band at Fronto-Central region. Lower 2 alpha, Beta and Gamma band power remained unchanged. Short term memory load has distinct electroencephalographic correlates resembling the mentally stressed state. This is evident from decreased power in Upper alpha band (corresponding to Alpha band of traditional EEG system) which is representative band of relaxed mental state. Fronto-temporal Theta power changes may reflect the encoding and execution of memory task.

Treatment of three patients with systemic mastocytosis with interferon alpha-2b.

PubMed

Worobec, A S; Kirshenbaum, A S; Schwartz, L B; Metcalfe, D D

1996-08-01

It has been reported that the administration of interferon alpha-2b is of potential benefit in the treatment of mastocytosis based on a single patient study (NEJM, Feb 27, 1992, 326(9):619-623). Following this report, we administered interferon alpha-2b at a dose of 4 to 5 million units per square meter of body surface area for at least 12 months to one patient with mastocytosis with an associated hematologic disorder (patient 1), one patient with aggressive systemic mastocytosis (patient 2), and one patient with indolent mastocytosis (patient 3). Patients were monitored with the following clinical and laboratory parameters: serial bone marrow biopsies and aspirates, patient log of histamine release attacks, medication dependency, plasma tryptase levels, serum lactate dehydrogenase (LDH) levels, white blood cell counts and differentials, extent of urticaria pigmentosa lesions, bony involvement, and extent of gastrointestinal involvement and hepatomegaly. We also examined the ability of interferon alpha-2b to inhibit recombinant human stem cell factor (rhSCF)-dependent mast cell proliferation from CD34+ bone marrow-derived cells. All patients demonstrated continued progression of disease in one or more clinical criteria at one year of therapy. Similarly, interferon alpha-2b did not inhibit the culture of mast cells from CD34+ bone marrow-derived cells in the presence of SCF. Thus, in our study of three patients with systemic mastocytosis, treatment with interferon alpha-2b was found to be ineffective in controlling progression of disease.

Alpha Channeling in Open-System Magnetic Devices

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fisch, Nathaniel

The Grant DE-SC0000736, Alpha Channeling in Open-System Magnetic Devices, is a continuation of the Grant DE-FG02-06ER54851, Alpha Channeling in Mirror Machines. In publications funded by DE-SC0000736, the grant DE-FG02-06ER54851 was actually credited. The key results obtained under Grant DE-SC0000736, Alpha Channeling in Open-System Magnetic Devices, appear in a series of publications. The earlier effort under DE-FG02- 06ER54851 was the subject of a previous Final Report. The theme of this later effort has been unusual confinement effects, or de-confinement effects, in open-field magnetic confinement devices. First, the possibilities in losing axisymmetry were explored. Then a number of issues in rotating plasmamore » were addressed. Most importantly, a spinoff application to plasma separations was recognized, which also resulted in a provisional patent application. (That provisional patent application, however, was not pursued further.) Alpha channeling entails injecting waves into magnetically confined plasma to release energy from one particular ion while ejecting that ion. The ejection of the ion is actually a concomitant effect in releasing energy from the ion to the wave. In rotating plasma, there is the opportunity to store the energy in a radial electric field rather than in waves. In other words, the ejected alpha particle loses its energy to the radial potential, which in turn produces plasma rotation. This is a very useful effect, since producing radial electric fields by other means are technologically more difficult. In fact, one can heat ions, and then eject them, to produce the desired radial field. In each case, there is a separation effect of different ions, which generalizes the original alpha-channeling concept of separating alpha ash from hydrogen. In a further generalization of the separation concept, a double-well filter represents a new way to produce high-throughput separations of ions, potentially useful for nuclear waste remediation.« less

HNF4alpha dysfunction as a molecular rational for cyclosporine induced hypertension.

PubMed

Niehof, Monika; Borlak, Jürgen

2011-01-27

Induction of tolerance against grafted organs is achieved by the immunosuppressive agent cyclosporine, a prominent member of the calcineurin inhibitors. Unfortunately, its lifetime use is associated with hypertension and nephrotoxicity. Several mechanism for cyclosporine induced hypertension have been proposed, i.e. activation of the sympathetic nervous system, endothelin-mediated systemic vasoconstriction, impaired vasodilatation secondary to reduction in prostaglandin and nitric oxide, altered cytosolic calcium translocation, and activation of the renin-angiotensin system (RAS). In this regard the molecular basis for undue RAS activation and an increased signaling of the vasoactive oligopeptide angiotensin II (AngII) remain elusive. Notably, angiotensinogen (AGT) is the precursor of AngII and transcriptional regulation of AGT is controlled by the hepatic nuclear factor HNF4alpha. To better understand the molecular events associated with cyclosporine induced hypertension, we investigated the effect of cyclosporine on HNF4alpha expression and activity and searched for novel HNF4alpha target genes among members of the RAS cascade. Using bioinformatic algorithm and EMSA bandshift assays we identified angiotensin II receptor type 1 (AGTR1), angiotensin I converting enzyme (ACE), and angiotensin I converting enzyme 2 (ACE2) as genes targeted by HNF4alpha. Notably, cyclosporine represses HNF4alpha gene and protein expression and its DNA-binding activity at consensus sequences to AGT, AGTR1, ACE, and ACE2. Consequently, the gene expression of AGT, AGTR1, and ACE2 was significantly reduced as evidenced by quantitative real-time RT-PCR. While RAS is composed of a sophisticated interplay between multiple factors we propose a decrease of ACE2 to enforce AngII signaling via AGTR1 to ultimately result in vasoconstriction and hypertension. Taken collectively we demonstrate cyclosporine to repress HNF4alpha activity through calcineurin inhibitor mediated inhibition of nuclear factor of activation of T-cells (NFAT) which in turn represses HNF4alpha that leads to a disturbed balance of RAS.

[Acetylcholine activation of alpha-ketoglutarate oxidation in liver mitochondria].

PubMed

Shostakovskaia, I V; Doliba, N M; Gordiĭ, S K; Babskiĭ, A M; Kondrashova, M N

1986-01-01

Activation of alpha-ketoglutarate oxidation in the rat liver mitochondria takes place 15 and 30 min after intraperitoneal injection of acetyl choline. This mediator in doses of 25, 50 and 100 micrograms per 100 g of body weight causes a pronounced stimulation of phosphorylation respiration rate and calcium capacity of mitochondria with alpha-ketoglutarate oxidation. Acetyl choline is found to have a moderate inhibitory action on oxidation of lower (physiological) concentrations of succinate. Its stimulating action on alpha-ketoglutarate oxidation is associated with activation of M-cholinoreceptors; atropine, a choline-blocker, removes completely this effect. It is supposed that alpha-ketoglutarate and succinate are included into the composition of two reciprocal hormonal-substrate nucleotide systems.

A high-speed digital camera system for the observation of rapid H-alpha fluctuations in solar flares

NASA Technical Reports Server (NTRS)

Kiplinger, Alan L.; Dennis, Brian R.; Orwig, Larry E.

1989-01-01

Researchers developed a prototype digital camera system for obtaining H-alpha images of solar flares with 0.1 s time resolution. They intend to operate this system in conjunction with SMM's Hard X Ray Burst Spectrometer, with x ray instruments which will be available on the Gamma Ray Observatory and eventually with the Gamma Ray Imaging Device (GRID), and with the High Resolution Gamma-Ray and Hard X Ray Spectrometer (HIREGS) which are being developed for the Max '91 program. The digital camera has recently proven to be successful as a one camera system operating in the blue wing of H-alpha during the first Max '91 campaign. Construction and procurement of a second and possibly a third camera for simultaneous observations at other wavelengths are underway as are analyses of the campaign data.

Contribution of alpha3(IV)alpha4(IV)alpha5(IV) Collagen IV to the Mechanical Properties of the Glomerular Basement Membrane

NASA Astrophysics Data System (ADS)

Gyoneva, Lazarina

The glomerular basement membrane (GBM) is a vital part of the blood-urine filtration barrier in the kidneys. In healthy GBMs, the main tension-resisting component is alpha3(IV)alpha4(IV)alpha5(IV) type IV collagen, but in some diseases it is replaced by other collagen IV isoforms. As a result, the GBM becomes leaky and disorganized, ultimately resulting in kidney failure. Our goal is to understanding the biomechanical aspects of the alpha3(IV)alpha4(IV)alpha5(IV) chains and how their absence could be responsible for (1) the initial injury to the GBM and (2) progression to kidney failure. A combination of experiments and computational models were designed for that purpose. A model basement membrane was used to compare experimentally the distensibility of tissues with the alpha3(IV)alpha4(IV)alpha5(IV) chains present and missing. The experiments showed basement membranes containing alpha3(IV)alpha4(IV)alpha5(IV) chains were less distensible. It has been postulated that the higher level of lateral cross-linking (supercoiling) in the alpha3(IV)alpha4(IV)alpha5(IV) networks contributes additional strength/stability to basement membranes. In a computational model of supercoiled networks, we found that supercoiling greatly increased the stiffness of collagen IV networks but only minimally decreased the permeability, which is well suited for the needs of the GBM. It is also known that the alpha3(IV)alpha4(IV)alpha5(IV) networks are more protected from enzymatic degradation, and we explored their significance in GBM remodeling. Our simulations showed that the more protected network was needed to prevent the system from entering a dangerous feedback cycle due to autoregulation mechanisms in the kidneys. Overall, the work adds to the evidence of biomechanical differences between the alpha3(IV)alpha4(IV)alpha5(IV) networks and other collagen IV networks, points to supercoiling as the main source of biomechanical differences, discusses the suitability of alpha3(IV)alpha4(IV)alpha5(IV) networks to meet the mechanics and permeability needs of the GBM, and explores the role of biomechanics and enzymatic digestion in GBM remodeling.

Anticipation and consumption of food each increase the concentration of neuroactive steroids in rat brain and plasma.

PubMed

Pisu, Maria Giuseppina; Floris, Ivan; Maciocco, Elisabetta; Serra, Mariangela; Biggio, Giovanni

2006-09-01

Stressful stimuli and anxiogenic drugs increase the plasma and brain concentrations of neuroactive steroids. Moreover, in rats trained to consume their daily meal during a fixed period, the anticipation of food is associated with changes in the function of various neurotransmitter systems. We have now evaluated the effects of anticipation and consumption of food in such trained rats on the plasma and brain concentrations of 3alpha-hydroxy-5alpha-pregnan-20-one (3alpha,5alpha-TH PROG) and 3alpha,21-dihydroxy-5alpha-pregnan-20-one (3alpha,5alpha-TH DOC), two potent endogenous positive modulators of type A receptors for gamma-aminobutyric acid (GABA). The abundance of these neuroactive steroids was increased in both the cerebral cortex and plasma of the rats during both food anticipation and consumption. In contrast, the concentration of their precursor, progesterone, was increased in the brain only during food consumption, whereas it was increased in plasma only during food anticipation. Intraperitoneal administration of the selective agonist abecarnil (0.1 mg/kg) 40 min before food presentation prevented the increase in the brain levels of 3alpha,5alpha-TH PROG and 3alpha,5alpha-TH DOC during food anticipation but not that associated with consumption. The change in emotional state associated with food anticipation may thus result in an increase in the plasma and brain levels of 3alpha,5alpha-TH PROG and 3alpha,5alpha-TH DOC in a manner sensitive to the activation of GABA(A) receptor-mediated neurotransmission. A different mechanism, insensitive to activation of such transmission, may underlie the changes in the concentrations of these neuroactive steroids during food consumption.

Glutathione regulation of redox-sensitive signals in tumor necrosis factor-{alpha}-induced vascular endothelial dysfunction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tsou, T.-C.; Yeh, S.C.; Tsai, F.-Y.

2007-06-01

We investigated the regulatory role of glutathione in tumor necrosis factor-alpha (TNF-{alpha})-induced vascular endothelial dysfunction as evaluated by using vascular endothelial adhesion molecule expression and monocyte-endothelial monolayer binding. Since TNF-{alpha} induces various biological effects on vascular cells, TNF-{alpha} dosage could be a determinant factor directing vascular cells into different biological fates. Based on the adhesion molecule expression patterns responding to different TNF-{alpha} concentrations, we adopted the lower TNF-{alpha} (0.2 ng/ml) to rule out the possible involvement of other TNF-{alpha}-induced biological effects. Inhibition of glutathione synthesis by L-buthionine-(S,R)-sulfoximine (BSO) resulted in down-regulations of the TNF-{alpha}-induced adhesion molecule expression and monocyte-endothelial monolayermore » binding. BSO attenuated the TNF-{alpha}-induced nuclear factor-kappaB (NF-{kappa}B) activation, however, with no detectable effect on AP-1 and its related mitogen-activated protein kinases (MAPKs). Deletion of an AP-1 binding site in intercellular adhesion molecule-1 (ICAM-1) promoter totally abolished its constitutive promoter activity and its responsiveness to TNF-{alpha}. Inhibition of ERK, JNK, or NF-{kappa}B attenuates TNF-{alpha}-induced ICAM-1 promoter activation and monocyte-endothelial monolayer binding. Our study indicates that TNF-{alpha} induces adhesion molecule expression and monocyte-endothelial monolayer binding mainly via activation of NF-{kappa}B in a glutathione-sensitive manner. We also demonstrated that intracellular glutathione does not modulate the activation of MAPKs and/or their downstream AP-1 induced by lower TNF-{alpha}. Although AP-1 activation by the lower TNF-{alpha} was not detected in our systems, we could not rule out the possible involvement of transiently activated MAPKs/AP-1 in the regulation of TNF-{alpha}-induced adhesion molecule expression.« less

Altered TNF-Alpha, Glucose, Insulin and Amino Acids in Islets Langerhans Cultured in a Microgravity Model System

NASA Technical Reports Server (NTRS)

Tobin, Brian W.; Leeper-Woodford, Sandra K.; Hashemi, Brian B.; Smith, Scott M.; Sams, Clarence F.

2001-01-01

The present studies were designed to determine effects of a microgravity model system upon lipopolysaccharide (LPS) stimulated tumor necrosis factor alpha (TNF-alpha) activity and indices of insulin and fuel homeostasis of pancreatic islets of Langerhans. Islets (1726+/-1 17,150 u IEU) from Wistar Furth rats were treated as: 1) HARV (High Aspect Ratio Vessel cell culture) , 2) HARV plus LPS, 3) static culture, 4) static culture plus LPS. TNF-alpha (L929 cytotoxicity assay) was significantly increased in LPS-induced HARV and static cultures, yet the increase was more pronounced in the static culture group (p<0.05). A decrease in insulin concentration was demonstrated in the LPS stimulated HARV culture (p<0.05). We observed a greater glucose concentration and increased disappearance of arginine in islets cultured in HARVs. While nitrogenous compound analysis indicated a ubiquitous reliance upon glutamine in all experimental groups, arginine was converted to ornithine at a two-fold greater rate in the islets cultured in the HARV microgravity model system (p<0.05). These studies demonstrate alterations in LPS induced TNF-alpha production of pancreatic islets of Langerhans, favoring a lesser TNF activity in the HARV. These alterations in fuel homeostasis may be promulgated by gravity averaged cell culture methods or by three dimensional cell assembly.

Multidetector system for nanosecond tagged neutron technology based on hardware selection of events

NASA Astrophysics Data System (ADS)

Karetnikov, M. D.; Korotkov, S. A.; Khasaev, T. O.

2016-09-01

At the T( d, n)He4 reaction a neutron is accompanied by an associated alpha-particle emitted in the opposite direction. A time and a direction of the neutron escape can be determined by measuring a time and coordinates of the alpha particle at the position-sensitive alpha-detector. The nanosecond tagged neutron technology (NTNT) based on this principle has great potentialities for various applications, e.g., for remote detection of explosives. A spectrum of gamma-rays emitted at the interaction of tagged neutrons with nuclei of chemical elements allows identify a chemical composition of an irradiated object. For practical realization of NTNT, a time resolution of recording the alpha-gamma coincidences should be close to 1 ns. The total intensity of signals can exceed 1 × 106 1/s from all gamma-detectors and 7 × 106 1/s from the alpha-detector. The processing of such stream of data without losses and distortion of information is one of challenging problems of NTNT. Several models of analog DAQ system based on hardware selection of events were devised and their characteristics are examined. The comparison with the digital DAQ systems demonstrated that the analog DAQ provides better timing parameters, lower power consumption, and higher maximum rate of useful events.

The molecular chaperone alphaA-crystallin enhances lens epithelial cell growth and resistance to UVA stress.

PubMed

Andley, U P; Song, Z; Wawrousek, E F; Bassnett, S

1998-11-20

alphaA-Crystallin (alphaA) is a member of the small heat shock protein (sHSP) family and has the ability to prevent denatured proteins from aggregating in vitro. Lens epithelial cells express relatively low levels of alphaA, but in differentiated fiber cells, alphaA is the most abundant soluble protein. The lenses of alphaA-knock-out mice develop opacities at an early age, implying a critical role for alphaA in the maintenance of fiber cell transparency. However, the function of alpha-crystallin in the lens epithelium is unknown. To investigate the physiological function of alphaA in lens epithelial cells, we used the following two systems: alphaA knock-out (alphaA(-/-)) mouse lens epithelial cells and human lens epithelial cells that overexpress alphaA. The growth rate of alphaA(-/-) mouse lens epithelial cells was reduced by 50% compared with wild type cells. Cell cycle kinetics, measured by fluorescence-activated cell sorter analysis of propidium iodide-stained cells, indicated a relative deficiency of alphaA(-/-) cells in the G2/M phases. Exposure of mouse lens epithelial cells to physiological levels of UVA resulted in an increase in the number of apoptotic cells in the cultures. Four hours after irradiation the fraction of apoptotic cells in the alphaA(-/-) cultures was increased 40-fold over wild type. In cells lacking alphaA, UVA exposure modified F-actin, but actin was protected in cells expressing alphaA. Stably transfected cell lines overexpressing human alphaA were generated by transfecting extended life span human lens epithelial cells with the mammalian expression vector construct pCI-neoalphaA. Cells overexpressing alphaA were resistant to UVA stress, as determined by clonogenic survival. alphaA remained cytoplasmic after exposure to either UVA or thermal stress indicating that, unlike other sHSPs, the protective effect of alphaA was not associated with its relocalization to the nucleus. These results indicate that alphaA has important cellular functions in the lens over and above its well characterized role in refraction.

Lipopolysaccharide mitagates methamphetamine-induced striatal dopamine depletion via modulating local TNF-alpha and dopamine transporter expression.

PubMed

Lai, Yu-Ting; Tsai, Yen-Ping N; Cherng, Chianfang G; Ke, Jing-Jer; Ho, Ming-Che; Tsai, Chia-Wen; Yu, Lung

2009-04-01

Systemic lipopolysaccharide (LPS) treatment may affect methamphetamine (MA)-induced nigrostriatal dopamine (DA) depletion. This study was undertaken to determine the critical time window for the protective effects of LPS treatment and the underlying mechanisms. An LPS injection (1 mg/kg) 72 h before or 2 h after MA treatment [three consecutive, subcutaneous injections of MA (10 mg/kg each) at 2-h intervals] diminished the MA-induced DA depletion in mouse striatum. Such an LPS-associated effect was independent of MA-produced hyperthermia. TNF-alpha, IL-1beta, IL-6 expressions were all elevated in striatal tissues following a systemic injection with LPS, indicating that peripheral LPS treatment affected striatal pro-inflammatory cytokine expression. Striatal TNF-alpha expression was dramatically increased at 72 and 96 h after the MA treatment, while such TNF-alpha elevation was abolished by the LPS pretreatment protocol. Moreover, MA-produced activation of nuclear NFkappaB, a transcription factor following TNF-alpha activation, in striatum was abolished by the LPS (1 mg/kg) pretreatment. Furthermore, thalidomide, a TNF-alpha antagonist, treatment abolished the LPS pretreatment-associated protective effects. Pretreatment with mouse recombinant TNF-alpha in striatum diminished the MA-produced DA depletion. Finally, single LPS treatment caused a rapid down-regulation of dopamine transporter (DAT) in striatum. Taken together, we conclude that peripheral LPS treatment protects nigrostriatal DA neurons against MA-induced toxicity, in part, by reversing elevated TNF-alpha expression and subsequent signaling cascade and causing a rapid DAT down-regulation in striatum.

Transport of pyruvate and lactate in yeast mitochondria.

PubMed

Briquet, M

1977-02-07

Evidence for the existence of mediated transport of pyruvate and lactate in isolated mitochondria of Saccharomyces cerevisiae is presented. 1. The mitochondrial oxidation of pyruvate is specifically inhibited by the monocarboxylic oxoacids alpha-ketoisocaproate and by alpha-cyano-3-hydroxycinnamate, while pyruvate and malate dehydrogenases activities are not inhibited. 2. The stimulation of the mitochondrial oxidations of succinate, alpha-ketoglutarate and citrate by pyruvate are also inhibited by alpha-cyano-3-hydroxycinnamate. 3. The [14C]pyruvate uptake by yeast mitochondria follows saturation kinetics and is completely inhibited by alpha-cyano-3-hydroxycinnamate. 4. Large amplitude passive swellings of mitochondria of the wild type and of cytoplasmic rho- and rho-n mutants are induced by isoosmotic ammonium pyruvate and lactate. These pH-dependent swellings are inhibited by alpha-cyano-3-hydroxycinnamate suggesting that the carrier system is not coded by mitochondrial DNA.

An airborne system for vortex flow visualization on the F-18 high-alpha research vehicle

NASA Technical Reports Server (NTRS)

Curry, Robert E.; Richwine, David M.

1988-01-01

A flow visualization system for the F-18 high-alpha research vehicle is described which allows direct observation of the separated vortex flows over a wide range of flight conditions. The system consists of a smoke generator system, on-board photographic and video systems, and instrumentation. In the present concept, smoke is entrained into the low-pressure vortex core, and vortice breakdown is indicated by a rapid diffusion of the smoke. The resulting pattern is observed using photographic and video images and is correlated with measured flight conditions.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lopez-Ruiz, R.; Nagy, A.; Romera, E.

A two-parameter family of complexity measures C-tilde{sup ({alpha},{beta})} based on the Renyi entropies is introduced and characterized by a detailed study of its mathematical properties. This family is the generalization of a continuous version of the Lopez-Ruiz-Mancini-Calbet complexity, which is recovered for {alpha}=1 and {beta}=2. These complexity measures are obtained by multiplying two quantities bringing global information on the probability distribution defining the system. When one of the parameters, {alpha} or {beta}, goes to infinity, one of the global factors becomes a local factor. For this special case, the complexity is calculated on different quantum systems: H-atom, harmonic oscillator, andmore » square well.« less

Improving the limits of detection of low background alpha emission measurements

NASA Astrophysics Data System (ADS)

McNally, Brendan D.; Coleman, Stuart; Harris, Jack T.; Warburton, William K.

2018-01-01

Alpha particle emission - even at extremely low levels - is a significant issue in the search for rare events (e.g., double beta decay, dark matter detection). Traditional measurement techniques require long counting times to measure low sample rates in the presence of much larger instrumental backgrounds. To address this, a commercially available instrument developed by XIA uses pulse shape analysis to discriminate alpha emissions produced by the sample from those produced by other surfaces of the instrument itself. Experience with this system has uncovered two residual sources of background: cosmogenics and radon emanation from internal components. An R&D program is underway to enhance the system and extend the pulse shape analysis technique further, so that these residual sources can be identified and rejected as well. In this paper, we review the theory of operation and pulse shape analysis techniques used in XIA's alpha counter, and briefly explore data suggesting the origin of the residual background terms. We will then present our approach to enhance the system's ability to identify and reject these terms. Finally, we will describe a prototype system that incorporates our concepts and demonstrates their feasibility.

Distinct expression pattern of IFN-alpha and TNF-alpha in juvenile idiopathic arthritis synovial tissue.

PubMed

Gattorno, M; Chicha, L; Gregorio, A; Ferlito, F; Rossi, F; Jarrossay, D; Lanzavecchia, A; Martini, A; Manz, M G

2007-04-01

Recent laboratory and clinical data suggest that two prototype autoimmune diseases, systemic lupus erythematosus and rheumatoid arthritis are mainly driven by distinct cytokines, interferon (IFN)-alpha and tumour necrosis factor (TNF)-alpha, respectively. We here investigated the presence and characteristics of natural type I IFN-producing cells (IPCs), as well as IFN-alpha and TNF-alpha expression at sites of inflammation in juvenile idiopathic arthritis (JIA). Peripheral blood (PB) and synovial fluid (SF) mononuclear cells (MNCs) (n = 25 each) from JIA patients with active disease were studied. IPCs were identified as BCDA-2(+)CD123(+)HLA-DR(+)CD45RA(+) cells, and dendritic cells (DCs) as CD11c(+)CD14(-/low)lin(-) cells by flow cytometry. IPCs and DCs were analysed for Toll-like receptor-7 and -9 mRNA expression by real-time polymerase chain reaction. IFN-alpha was measured by enzyme-linked immunosorbent assay in serum, SF and in supernatants of influenza virus-infected, cultured IPCs. Synovial tissues of n = 6 additional JIA patients were analysed by immunohistochemistry using mAbs against CD123, IFN-alpha, TNF-alpha, CD3, CD19 and CD138. IPCs were enriched in SF MNCs compared with PB MNCs in all JIA patients. Influenza-induced, but no spontaneous IFN-alpha release was detected from SF IPCs, and serum and SF IFN-alpha levels were not elevated. Nonetheless, in synovial tissue IFN-alpha producing cells accumulated at inflammatory lymph-follicular-like structures, while TNF-alpha producing cells were mostly found at the lining and sublining layers. These data suggest that besides TNF-alpha-expressing cells, IFN-alpha-producing IPCs are involved in initiation, maintenance or regulation of the inflammatory response in JIA.

Functional assessment of recombinant human alpha(2)-adrenoceptor subtypes with cytosensor microphysiometry.

PubMed

Pihlavisto, M; Scheinin, M

1999-12-03

We applied the Cytosensor Microphysiometry system to study the three human alpha(2)-adrenoceptor subtypes, alpha(2A), alpha(2B) and alpha(2C), expressed in Chinese hamster ovary (CHO) cells, and assessed its potential in the quantitative monitoring of agonist activity. The natural full agonist, (-)-noradrenaline, was used to define agonist efficacy. The imidazole derivative dexmedetomidine was a potent full agonist of all three receptor subtypes. The imidazolines clonidine and UK 14,304 (5-bromo-N-(4, 5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine) appeared to be partial agonists at alpha(2B)-adrenoceptors (E(max) approximately 60% of (-)-noradrenaline) but full agonists at alpha(2A)- and alpha(2C)-adrenoceptors. The responses mediated by all three alpha(2)-adrenoceptor subtypes were partly inhibited by the sodium-hydrogen (Na(+)/H(+)) exchange inhibitor, MIA (5-(N-methyl-N-isobutyl)-amiloride). The agonist responses were totally abolished by pretreatment with pertussis toxin in cells with alpha(2A)- and alpha(2C)-adrenoceptors, and partly abolished in cells with alpha(2B)-adrenoceptors. The residual signal in alpha(2B)-cells was sensitive to the intracellular Ca(2+)chelator, BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid acetoxymethyl ester). Cholera toxin (which acts on G(s)-proteins) had no effect on the agonist responses. The results suggest that the extracellular acidification responses mediated by all three human alpha(2)-adrenoceptor subtypes are dependent on Na(+)/H(+)exchange and G(i/o) pathways, and that alpha(2B)-adrenoceptors are capable of coupling to another, G(i/o)-independent and Ca(2+)-dependent signaling pathway.

Bioactive interleukin-1alpha is cytolytically released from Candida albicans-infected oral epithelial cells.

PubMed

Dongari-Bagtzoglou, A; Kashleva, H; Villar, C Cunha

2004-12-01

Oral epithelial cells are primary targets of Candida albicans in the oropharynx and may regulate the inflammatory host response to this pathogen. This investigation studied the mechanisms underlying interleukin-1alpha (IL-1alpha) release by oral epithelial cells and the role of IL-1alpha in regulating the mucosal inflammatory response to C. albicans. Infected oral epithelial cells released processed IL-1alpha protein in culture supernatants. The IL-1alpha generated was stored intracellularly and was released upon cell lysis. This was further supported by the fact that different C. albicans strains induced variable IL-1alpha release, depending on their cytolytic activity. IL-1alpha from C. albicans-infected oral epithelial cells upregulated proinflammatory cytokine secretion (IL-8 and GM-CSF) in uninfected oral epithelial or stromal cells. Our studies suggest that production of IL-1alpha, IL-8 and GM-CSF may take place in the oral mucosa in response to lytic infection of epithelial cells with C. albicans. This process can act as an early innate immune surveillance system and may contribute to the clinicopathologic signs of infection in the oral mucosa.

The alpha(2a)-adrenergic receptor plays a protective role in mouse behavioral models of depression and anxiety.

PubMed

Schramm, N L; McDonald, M P; Limbird, L E

2001-07-01

The noradrenergic system is involved in the regulation of many physiological and psychological processes, including the modulation of mood. The alpha(2)-adrenergic receptors (alpha(2)-ARs) modulate norepinephrine release, as well as the release of serotonin and other neurotransmitters, and are therefore potential targets for antidepressant and anxiolytic drug development. The current studies were undertaken to examine the role of the alpha(2A) subtype of alpha(2)-AR in mouse behavioral models of depression and anxiety. We have observed that the genetic knock-out of the alpha(2A)-AR makes mice less active in a modified version of Porsolt's forced swim test and insensitive to the antidepressant effects of the tricyclic drug imipramine in this paradigm. Furthermore, alpha(2A)-AR knock-out mice appear more anxious than wild-type C57 Bl/6 mice in the rearing and light-dark models of anxiety after injection stress. These findings suggest that the alpha(2A)-AR may play a protective role in some forms of depression and anxiety and that the antidepressant effects of imipramine may be mediated by the alpha(2A)-AR.

Limits on the time variation of the electromagnetic fine-structure constant in the low energy limit from absorption lines in the spectra of distant quasars.

PubMed

Srianand, R; Chand, H; Petitjean, P; Aracil, B

2004-03-26

We present the results of a detailed many-multiplet analysis performed on a new sample of Mg ii systems observed in high quality quasar spectra obtained using the Very Large Telescope. The weighted mean value of the variation in alpha derived from our analysis over the redshift range 0.4

Prediction of alpha factor values for fine pore aeration systems.

PubMed

Gillot, S; Héduit, A

2008-01-01

The objective of this work was to analyse the impact of different geometric and operating parameters on the alpha factor value for fine bubble aeration systems equipped with EPDM membrane diffusers. Measurements have been performed on nitrifying plants operating under extended aeration and treating mainly domestic wastewater. Measurements performed on 14 nitrifying plants showed that, for domestic wastewater treatment under very low F/M ratios, the alpha factor is comprised between 0.44 and 0.98. A new composite variable (the Equivalent Contact Time, ECT) has been defined and makes it possible for a given aeration tank, knowing the MCRT, the clean water oxygen transfer coefficient and the supplied air flow rate, to predict the alpha factor value. ECT combines the effect on mass transfer of all generally accepted factors affecting oxygen transfer performances (air flow rate, diffuser submergence, horizontal flow). (c) IWA Publishing 2008.

Macrophage inflammatory protein-1alpha: a link between innate immunity and familial Mediterranean fever?

PubMed

Dizdar, Omer; Kalyoncu, Umut; Karadag, Omer; Akdogan, Ali; Kiraz, Sedat; Ertenli, Ihsan; Barista, Ibrahim; Calguneri, Meral

2007-01-01

The aim of this study is to investigate the relationship between chemokines and the inflammation in Familial Mediterranean Fever (FMF). Forty-nine patients with FMF (41 in remission and 8 in acute attack period) and 20 healthy controls were included in the study. Serum levels of macrophage inflammatory protein-1alpha (MIP-1alpha) were assessed in the patients and the controls, along with other parameters of disease activity, i.e., fibrinogen, C-reactive protein and erythrocyte sedimentation rate. Serum MIP-1alpha levels of the patients with FMF in acute attack period were significantly higher than the patients in remission and healthy controls (p=0.02 and p=0.038, respectively). MIP-1alpha levels were weakly correlated with CRP (r=0.32, p=0.032) levels. MIP-1alpha may have a role in the pathogenesis of FMF attacks. MIP-1alpha and other chemokines may constitute a link between the innate immune system and FMF.

Absence of detectable immunoreactive alpha melanocyte stimulating hormone in plasma in various types of Cushing's disease.

PubMed

Croughs, R J; Thijssen, J H; Mol, J A

1991-03-01

We have measured alpha-MSH in plasma of normal subjects and subjects with various diseases of the pituitary-adrenocortical system using a radioimmunoassay with a sensitivity of 1.2 pmol/l. No alpha-MSH could be detected in plasma of normal subjects (n = 6), in plasma of patients with Addison's disease (n = 3), Nelson's syndrome (n = 2), bromocriptine responsive (n = 2) and unresponsive (n = 5) Cushing's disease and in plasma of psychiatric patients on chronic treatment with the dopamine antagonist haloperidol (n = 5). Plasma alpha-MSH remained undetectable in 2 patients with Cushing's disease after iv injection of 60 micrograms/kg haloperidol. In contrast, alpha-MSH was detectable in plasma of normal dogs (n = 2) and dogs with pituitary dependent hyperadrenocorticism (n = 2), whereas the iv injection of halo peridol was associated with a rise of plasma alpha-MSH. Thus we are unable to detect circulating alpha-MSH in man despite the use of a sensitive radioimmunoassay.

Mutants in the mouse NuRD/Mi2 component P66alpha are embryonic lethal.

PubMed

Marino, Susan; Nusse, Roel

2007-06-13

The NuRD/Mi2 chromatin complex is involved in histone modifications and contains a large number of subunits, including the p66 protein. There are two mouse and human p66 paralogs, p66alpha and p66beta. The functions of these genes are not clear, in part because there are no mutants available, except in invertebrate model systems. We made loss of function mutants in the mouse p66alpha gene (mp66alpha, official name Gatad2a, MGI:2384585). We found that mp66alpha is essential for development, as mutant embryos die around day 10 of embryogenesis. The gene is not required for normal blastocyst development or for implantation. The phenotype of mutant embryos and the pattern of gene expression in mutants are consistent with a role of mp66alpha in gene silencing. mp66alpha is an essential gene, required for early mouse development. The lethal phenotype supports a role in execution of methylated DNA silencing.

Ultraviolet observations of alpha Aurigae from Copernicus

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dupree, A. K.

1975-08-01

Emission lines of L$alpha$ (1215.67 A) and O vi (1031.94 A) were detected in the spectroscopic binary $alpha$ Aur (Capella) with the Princeton experiment on Copernicus. Temperatures of the emitting regions are inferred to be in excess of 3times10$sup 5$ K. The temperature and emission measure are consistent with atmosphere is expanding with velocities approx.20 to 100 km s$sup -1$. Such expansion can lead to material within the binary system. The density of interstellar hydrogen inferred from absorption of stellar L$alpha$ appears to be approx.0.01 hydrogen atoms cm$sup -3$.

Localization of mRNA for CHRNA7 in human fetal brain.

PubMed

Agulhon, C; Abitbol, M; Bertrand, D; Malafosse, A

1999-08-02

The aim of this study was to determine the regional distribution in situ of the mRNA for the alpha 7 subunit of the neuronal nicotinic acetylcholine receptor in human fetal brain. We found high levels of alpha 7 gene expression in nuclei that receive sensory information, such as those of the neocortex and hippocampus, the thalamic nuclei, the reticular thalamic nucleus, the pontine nuclei and the superior olive complex. These data support a possible regulatory function for alpha 7-containing receptors in sensory processing, which may be involved in the pathological physiology of schizophrenia and autism. Early alpha 7 gene expression is also consistent with a morphogenetic role for alpha 7 receptors in central nervous system development.

Posttranscriptional regulation of alpha-amylase II-4 expression by gibberellin in germinating rice seeds.

PubMed

Nanjo, Yohei; Asatsuma, Satoru; Itoh, Kimiko; Hori, Hidetaka; Mitsui, Toshiaki; Fujisawa, Yukiko

2004-06-01

Hormonal regulation of expression of alpha-amylase II-4 that lacks the gibberellin-response cis-element (GARE) in the promoter region of the gene was studied in germinating rice (Oryza sativa L.) seeds. Temporal and spatial expression of alpha-amylase II-4 in the aleurone layer were essentially identical to those of alpha-amylase I-1 whose gene contains GARE, although these were distinguishable in the embryo tissues at the early stage of germination. The gibberellin-responsible expression of alpha-amylase II-4 was also similar to that of alpha-amylase I-1. However, the level of alpha-amylase II-4 mRNA was not increased by gibberellin, indicating that the transcriptional enhancement of alpha-amylase II-4 expression did not occur in the aleurone. Gibberellin stimulated the accumulation of 45Ca2+ into the intracellular secretory membrane system. In addition, several inhibitors for Ca2+ signaling, such as EGTA, neomycin, ruthenium red (RuR), and W-7 prevented the gibberellin-induced expression of alpha-amylase II-4 effectively. While the gibberellin-induced expression of alpha-amylase II-4 occurred normally in the aleurone layer of a rice dwarf mutant d1 which is defective in the alpha subunit of the heterotrimeric G protein. Based on these results, it was concluded that the posttranscriptional regulation of alpha-amylase II-4 expression by gibberellin operates in the aleurone layer of germinating rice seed, which is mediated by Ca2+ but not the G protein.

Computer assisted performance tests of the Lyman Alpha Coronagraph

NASA Technical Reports Server (NTRS)

Parkinson, W. H.; Kohl, J. L.

1979-01-01

Preflight calibration and performance tests of the Lyman Alpha Coronagraph rocket instrument in the laboratory, with the experiment in its flight configuration and illumination levels near those expected during flight were successfully carried out using a pulse code modulation telemetry system simulator interfaced in real time to a PDP 11/10 computer system. Post acquisition data reduction programs developed and implemented on the same computer system aided in the interpretation of test and calibration data.

Diffusion coefficients in systems with inclusion compounds. 1. alpha. -Cyclodextrin-L-phenylalanine-water at 25 degree C

DOE Office of Scientific and Technical Information (OSTI.GOV)

Paduano, L.; Sartorio, R.; Vitagliano, V.

Diffusion coefficients in the ternary system {alpha}-cyclodextrin (at one concentration)-L-phenylalanine (at four concentrations)-water have been measured by using the Gouy interferometric technique. The effect of the inclusion equilibrium on the cross-term diffusion coefficients was observed. The measured diffusion coefficients in the ternary systems were used to calculate values of the binding constants. These values are in good agreement with the value obtained from calorimetric studies.

Research flight-control system development for the F-18 high alpha research vehicle

NASA Technical Reports Server (NTRS)

Pahle, Joseph W.; Powers, Bruce; Regenie, Victoria; Chacon, Vince; Degroote, Steve; Murnyak, Steven

1991-01-01

The F-18 high alpha research vehicle was recently modified by adding a thrust vectoring control system. A key element in the modification was the development of a research flight control system integrated with the basic F-18 flight control system. Discussed here are design requirements, system development, and research utility of the resulting configuration as an embedded system for flight research in the high angle of attack regime. Particular emphasis is given to control system modifications and control law features required for high angle of attack flight. Simulation results are used to illustrate some of the thrust vectoring control system capabilities and predicted maneuvering improvements.

Alpha-Synuclein Expression Restricts RNA Viral Infections in the Brain.

PubMed

Beatman, Erica L; Massey, Aaron; Shives, Katherine D; Burrack, Kristina S; Chamanian, Mastooreh; Morrison, Thomas E; Beckham, J David

2015-12-30

We have discovered that native, neuronal expression of alpha-synuclein (Asyn) inhibits viral infection, injury, and disease in the central nervous system (CNS). Enveloped RNA viruses, such as West Nile virus (WNV), invade the CNS and cause encephalitis, yet little is known about the innate neuron-specific inhibitors of viral infections in the CNS. Following WNV infection of primary neurons, we found that Asyn protein expression is increased. The infectious titer of WNV and Venezuelan equine encephalitis virus (VEEV) TC83 in the brains of Asyn-knockout mice exhibited a mean increase of 10(4.5) infectious viral particles compared to the titers in wild-type and heterozygote littermates. Asyn-knockout mice also exhibited significantly increased virus-induced mortality compared to Asyn heterozygote or homozygote control mice. Virus-induced Asyn localized to perinuclear, neuronal regions expressing viral envelope protein and the endoplasmic reticulum (ER)-associated trafficking protein Rab1. In Asyn-knockout primary neuronal cultures, the levels of expression of ER signaling pathways, known to support WNV replication, were significantly elevated before and during viral infection compared to those in Asyn-expressing primary neuronal cultures. We propose a model in which virus-induced Asyn localizes to ER-derived membranes, modulates virus-induced ER stress signaling, and inhibits viral replication, growth, and injury in the CNS. These data provide a novel and important functional role for the expression of native alpha-synuclein, a protein that is closely associated with the development of Parkinson's disease. Neuroinvasive viruses such as West Nile virus are able to infect neurons and cause severe disease, such as encephalitis, or infection of brain tissue. Following viral infection in the central nervous system, only select neurons are infected, implying that neurons exhibit innate resistance to viral infections. We discovered that native neuronal expression of alpha-synuclein inhibited viral infection in the central nervous system. When the gene for alpha-synuclein was deleted, mice exhibited significantly decreased survival, markedly increased viral growth in the brain, and evidence of increased neuron injury. Virus-induced alpha-synuclein localized to intracellular neuron membranes, and in the absence of alpha-synuclein expression, specific endoplasmic reticulum stress signaling events were significantly increased. We describe a new neuron-specific inhibitor of viral infections in the central nervous system. Given the importance of alpha-synuclein as a cause of Parkinson's disease, these data also ascribe a novel functional role for the native expression of alpha-synuclein in the CNS. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Dehydroepiandrosterone administration counteracts oxidative imbalance and advanced glycation end product formation in type 2 diabetic patients.

PubMed

Brignardello, Enrico; Runzo, Cristina; Aragno, Manuela; Catalano, Maria Graziella; Cassader, Maurizio; Perin, Paolo Cavallo; Boccuzzi, Giuseppe

2007-11-01

Dehydroepiandrosterone (DHEA) has been shown to prevent oxidative stress in several in vivo and in vitro models. This study aimed to evaluate the effects of DHEA administration on oxidative stress, pentosidine concentration, and tumor necrosis factor (TNF)-alpha/TNF-alpha receptor system activity in patients with type 2 diabetes. Twenty patients were enrolled in the study and randomly assigned to the DHEA (n = 10) or placebo (n = 10) group. Twenty healthy sex- and age-matched subjects with normal glucose levels served as control subjects. DHEA was given as a single daily dose of 50 mg for 12 weeks. Oxidative stress parameters were significantly higher in diabetic patients versus control subjects. Pentosidine levels, as well as soluble TNF receptor (sTNF-R)I and sTNF-RII, were also higher in diabetic patients. After DHEA, plasma levels of reactive oxygen species and hydroxynonenal dropped by 53 and 47%, respectively, whereas the nonenzymatic antioxidants glutathione and vitamin E increased (+38 and +76%, respectively). The same changes in oxidative parameters were detected in peripheral blood mononuclear cells (PBMCs). DHEA treatment also induced a marked decrease of pentosidine plasma concentration in diabetic patients (-50%). Moreover, the TNF-alpha/TNF-alpha receptor system was shown to be less activated after DHEA treatment, in both plasma and PBMCs. Data indicate that DHEA treatment ameliorates the oxidative imbalance induced by hyperglycemia, downregulates the TNF-alpha/TNF-alpha receptor system, and prevents advanced glycation end product formation, suggesting a beneficial effect on the onset and/or progression of chronic complications in type 2 diabetic patients.

Development of a homogeneous pulse shape discriminating flow-cell radiation detection system

NASA Astrophysics Data System (ADS)

Hastie, K. H.; DeVol, T. A.; Fjeld, R. A.

1999-02-01

A homogeneous flow-cell radiation detection system which utilizes coincidence counting and pulse shape discrimination circuitry was assembled and tested with five commercially available liquid scintillation cocktails. Two of the cocktails, Ultima Flo (Packard) and Mono Flow 5 (National Diagnostics) have low viscosities and are intended for flow applications; and three of the cocktails, Optiphase HiSafe 3 (Wallac), Ultima Gold AB (Packard), and Ready Safe (Beckman), have higher viscosities and are intended for static applications. The low viscosity cocktails were modified with 1-methylnaphthalene to increase their capability for alpha/beta pulse shape discrimination. The sample loading and pulse shape discriminator setting were optimized to give the lowest minimum detectable concentration for alpha radiation in a 30 s count time. Of the higher viscosity cocktails, Optiphase HiSafe 3 had the lowest minimum detectable activities for alpha and beta radiation, 0.2 and 0.4 Bq/ml for 233U and 90Sr/ 90Y, respectively, for a 30 s count time. The sample loading was 70% and the corresponding alpha/beta spillover was 5.5%. Of the low viscosity cocktails, Mono Flow 5 modified with 2.5% (by volume) 1-methylnaphthalene resulted in the lowest minimum detectable activities for alpha and beta radiation; 0.3 and 0.5 Bq/ml for 233U and 90Sr/ 90Y, respectively, for a 30 s count time. The sample loading was 50%, and the corresponding alpha/beta spillover was 16.6%. HiSafe 3 at a 10% sample loading was used to evaluate the system under simulated flow conditions.

Catecholamine autotoxicity. Implications for pharmacology and therapeutics of Parkinson disease and related disorders.

PubMed

Goldstein, David S; Kopin, Irwin J; Sharabi, Yehonatan

2014-12-01

Several neurodegenerative diseases involve loss of catecholamine neurons-Parkinson disease is a prototypical example. Catecholamine neurons are rare in the nervous system, and why they are vulnerable in PD and related disorders has been mysterious. Accumulating evidence supports the concept of "autotoxicity"-inherent cytotoxicity of catecholamines and their metabolites in the cells in which they are produced. According to the "catecholaldehyde hypothesis" for the pathogenesis of Parkinson disease, long-term increased build-up of 3,4-dihydroxyphenylacetaldehyde (DOPAL), the catecholaldehyde metabolite of dopamine, causes or contributes to the eventual death of dopaminergic neurons. Lewy bodies, a neuropathologic hallmark of PD, contain precipitated alpha-synuclein. Bases for the tendency of alpha-synuclein to precipitate in the cytoplasm of catecholaminergic neurons have also been mysterious. Since DOPAL potently oligomerizes and aggregates alpha-synuclein, the catecholaldehyde hypothesis provides a link between alpha-synucleinopathy and catecholamine neuron loss in Lewy body diseases. The concept developed here is that DOPAL and alpha-synuclein are nodes in a complex nexus of interacting homeostatic systems. Dysfunctions of several processes, including decreased vesicular sequestration of cytoplasmic catecholamines, decreased aldehyde dehydrogenase activity, and oligomerization of alpha-synuclein, lead to conversion from the stability afforded by negative feedback regulation to the instability, degeneration, and system failure caused by induction of positive feedback loops. These dysfunctions result from diverse combinations of genetic predispositions, environmental exposures, stress, and time. The notion of catecholamine autotoxicity has several implications for treatment, disease modification, and prevention. Conversely, disease modification clinical trials would provide key tests of the catecholaldehyde hypothesis. Published by Elsevier Inc.

Catecholamine autotoxicity. Implications for pharmacology and therapeutics of Parkinson disease and related disorders☆

PubMed Central

Goldstein, David S.; Kopin, Irwin J.; Sharabi, Yehonatan

2015-01-01

Several neurodegenerative diseases involve loss of catecholamine neurons—Parkinson disease is a prototypical example. Catecholamine neurons are rare in the nervous system, and why they are vulnerable in PD and related disorders has been mysterious. Accumulating evidence supports the concept of “autotoxicity”—inherent cytotoxicity of catecholamines and their metabolites in the cells in which they are produced. According to the “catecholaldehyde hypothesis” for the pathogenesis of Parkinson disease, long-term increased build-up of 3,4-dihydroxyphenylacetaldehyde (DOPAL), the catecholaldehyde metabolite of dopamine, causes or contributes to the eventual death of dopaminergic neurons. Lewy bodies, a neuropathologic hallmark of PD, contain precipitated alpha-synuclein. Bases for the tendency of alpha-synuclein to precipitate in the cytoplasm of catecholaminergic neurons have also been mysterious. Since DOPAL potently oligomerizes and aggregates alpha-synuclein, the catecholaldehyde hypothesis provides a link between alpha-synucleinopathy and catecholamine neuron loss in Lewy body diseases. The concept developed here is that DOPAL and alpha-synuclein are nodes in a complex nexus of interacting homeostatic systems. Dysfunctions of several processes, including decreased vesicular sequestration of cytoplasmic catecholamines, decreased aldehyde dehydrogenase activity, and oligomerization of alpha-synuclein, lead to conversion from the stability afforded by negative feedback regulation to the instability, degeneration, and system failure caused by induction of positive feedback loops. These dysfunctions result from diverse combinations of genetic predispositions, environmental exposures, stress, and time. The notion of catecholamine autotoxicity has several implications for treatment, disease modification, and prevention. Conversely, disease modification clinical trials would provide key tests of the catecholaldehyde hypothesis. PMID:24945828

[Study of the interaction of alpha-tocopherol with phospholipids, fatty acids, and their oxygenated derivatives by (31)P-NMR spectroscopy].

PubMed

Chudinova, V V; Zakharova, E I; Alekseev, S M; Chupin, V V; Evstigneeva, R P

1993-02-01

Interaction of alpha-tocopherol with phospholipids, oleic, ricinoleic acids and linoleic acid hydroperoxides was investigated by means of 31P NMR spectroscopy on a model artificial membranes containing egg phosphatidylcholine and lysophosphatidylcholine. alpha-Tocopherol was shown to support the bilayer organization of lysophospholipids, whereas its introduction into the lecithin-water system stimulated the hexagonal phase formation. Free fatty acids exhibited a synergism to alpha-tocopherol, the effect of the hexagonal phase formation being at most increased by oxygenated acids--ricinoleic acid and linoleic acid hydroperoxides. In accordance with the experimental data, a conclusion about modifying and structuring action of alpha-tocopherol was made. Origin of the alpha-tocopherol's modulating effect on the membrane structure and a possible role of hexagonal phase forming upon its action in the course of peroxidation of lipids was discussed.

Variaciones seculares de período en las RR Lyrae de ω~Centauri

NASA Astrophysics Data System (ADS)

Marraco, H. G.; Milesi, G. E.

Utilizando 689 observaciones de 35 estrellas RR Lyrae del cúmulo globular ω Centauri hemos obtenido nuevas determinaciones de sus períodos y sus correspondientes variaciones seculares. Las observaciones fueron obtenidas de la literatura con la excepción de un grupo 66 determinaciones que se presentan por vez primera aquí. Utilizando el parámetro testigo σ descripto en Marraco & Muzzio (Publ. Astron. Soc. Pacific 92, 700, 1980), hemos realizado un ajuste bidimensional en P y β (donde β es la variación secular del período). Con este fin la totalidad de las 689 observaciones fueron llevadas a un sistema fotométrico común. Para esto se realizó un cuidadoso análisis de los numerosos errores en la identificación de las estrellas de las series de comparación. Los resultados de los ajustes bidimensionales fueron analizados utilizando técnicas de procesamiento de imágenes. Con este fin el parámetro de ajuste σ fue representado como función de P y β. En las imágenes resultantes se buscaron los mínimos y al menor de ellos se lo aceptó como período instantáneo verdadero y su variación secular β. La determinación precisa de cada parámetro se realizó mediante ajuste de gaussianas y se determinaron sus errores. A modo de ejemplo la variable #8 fue analizada en una matriz de 501 × 501 elementos representando el parámetro σ para valores comprendidos entre 0,521034 < P < 0,521534 dias y -150×10-10 < β < +150×10-10 dias/dia. El mejor período instantáneo (correspondiente a la época DJ=2.426.908) y su variación secular son P = 0,5212859±0,0000001 días y β 14,012±,010×10-10 días/día respectivamente. Con estos valores el parámetro testigo resulta σ= 0,127 . Si no se tiene en cuenta la variación secular del período y se busca aquél de mejor ajuste para β = 0, se obtiene P = 0,5212960 días, pero entonces el parámetro de ajuste resulta tan alto como σ = 0,23 .

The end of the White Dwarf Cooling Sequence of NGC 6752

NASA Astrophysics Data System (ADS)

Bedin, Luigi

2017-08-01

We propose to study the last HST-accessible white dwarf (WD) cooling sequence (CS) for a nearby globular cluster (GC), the chemically complex, extreme blue horizontal branch cluster NGC 6752. Over 97% of stars end their lives as WDs, and the WD CS provides constraints not only on the age, but also potentially the star formation history of a GC. The CS of WDs also lies in the least-explored region of the color-magnitude diagram of old stellar populations. Recent deep imaging with HST has successfully reached the end of the WD CS in only three classical old GCs, M4, NGC 6397 and 47 Tuc, and reveals an unexpectedly complex, and double-peaked, WD CS in the metal rich old open cluster NGC 6791. One more investigation is in progress on the massive globular Omega Centauri, where over 14 sub-populations are known to exist.While almost every cluster is known to host multiple populations, every single cluster is unique. NGC 6752 is a bridge between the relatively simple globular clusters, and Omega Cen, the most complex globular cluster known. NGC 6752 has an extended blue horizontal branch, a collapsed core and 3 chemically distinct populations. It is our last chance to add diversity to our very limited sample of WD CS, so far containing only 3 globular clusters, one old open cluster, and the complex Omega Cen system. We need to undertake this investigation while HST is still operational, as there is no foreseeable opportunity in the post-HST era to have one extra WD CS in the homogeneus optical photometric system of HST.

Interaction of plant chimeric calcium/calmodulin-dependent protein kinase with a homolog of eukaryotic elongation factor-1alpha

NASA Technical Reports Server (NTRS)

Wang, W.; Poovaiah, B. W.

1999-01-01

A chimeric Ca2+/calmodulin-dependent protein kinase (CCaMK) was previously cloned and characterized in this laboratory. To investigate the biological functions of CCaMK, the yeast two-hybrid system was used to isolate genes encoding proteins that interact with CCaMK. One of the cDNA clones obtained from the screening (LlEF-1alpha1) has high similarity with the eukaryotic elongation factor-1alpha (EF-1alpha). CCaMK phosphorylated LlEF-1alpha1 in a Ca2+/calmodulin-dependent manner. The phosphorylation site for CCaMK (Thr-257) was identified by site-directed mutagenesis. Interestingly, Thr-257 is located in the putative tRNA-binding region of LlEF-1alpha1. An isoform of Ca2+-dependent protein kinase (CDPK) phosphorylated multiple sites of LlEF-1alpha1 in a Ca2+-dependent but calmodulin-independent manner. Unlike CDPK, CCaMK phosphorylated only one site, and this site is different from CDPK phosphorylation sites. This suggests that the phosphorylation of EF-1alpha by these two kinases may have different functional significance. Although the phosphorylation of LlEF-1alpha1 by CCaMK is Ca2+/calmodulin-dependent, in vitro binding assays revealed that CCaMK binds to LlEF-1alpha1 in a Ca2+-independent manner. This was further substantiated by coimmunoprecipitation of CCaMK and EF-1alpha using the protein extract from lily anthers. Dissociation of CCaMK from EF-1alpha by Ca2+ and phosphorylation of EF-1alpha by CCaMK in a Ca2+/calmodulin-dependent manner suggests that these interactions may play a role in regulating the biological functions of EF-1alpha.

An alpha–gamma coincidence spectrometer based on the Photon–Electron Rejecting Alpha Liquid Scintillation (PERALS®) system

DOE PAGES

Cadieux, J. R.; Fugate, G. A.; King, III, G. S.

2015-02-07

Here, an alpha–gamma coincidence spectrometer has been developed for the measurement of selected actinide isotopes in the presence of high beta/gamma fields. The system is based on a PERALS® liquid scintillation counter for beta/alpha discrimination and was successfully tested with both high purity germanium and bismuth germanate, gamma-ray detectors using conventional analog electronics.

Alpha-canonical form representation of the open loop dynamics of the Space Shuttle main engine

NASA Technical Reports Server (NTRS)

Duyar, Almet; Eldem, Vasfi; Merrill, Walter C.; Guo, Ten-Huei

1991-01-01

A parameter and structure estimation technique for multivariable systems is used to obtain a state space representation of open loop dynamics of the space shuttle main engine in alpha-canonical form. The parameterization being used is both minimal and unique. The simplified linear model may be used for fault detection studies and control system design and development.

On the Biohabitability of M-dwarf Planets

NASA Astrophysics Data System (ADS)

Wandel, A.

2018-04-01

The recent detection of Earth-sized planets in the habitable zone of Proxima Centauri, Trappist-1, and many other nearby M-type stars has led to speculations whether liquid water and life actually exist on these planets. To a large extent, the answer depends on their yet unknown atmospheres, which may, however, be within observational reach in the near future by JWST, ELT, and other planned telescopes. We consider the habitability of planets of M-type stars in the context of their atmospheric properties, heat transport, and irradiation. Instead of the traditional definition of the habitable zone, we define the biohabitable zone, where liquid water and complex organic molecules can survive on at least part of the planetary surface. The atmospheric impact on the temperature is quantified in terms of the heating factor (a combination of greenhouse heating, stellar irradiation, albedo, etc.) and heat redistribution (horizontal energy transport). We investigate the biohabitable domain (where planets can support surface liquid water and organics) in terms of these two factors. Our results suggest that planets orbiting M-type stars may have life-supporting temperatures, at least on part of their surface, for a wide range of atmospheric properties. We apply this analyses to Proxima Cen b and the Trappist-1 system. Finally, we discuss the implications for the search of biosignatures and demonstrate how they may be used to estimate the abundance of photosynthesis and biotic planets.

Novel sustained-release dosage forms of proteins using polyglycerol esters of fatty acids.

PubMed

Yamagata, Y; Iga, K; Ogawa, Y

2000-02-03

In order to develop a novel delivery system for proteins based on polyglycerol esters of fatty acids (PGEFs), we studied a model system using interferon-alpha (IFN-alpha) as the test protein. A cylindrical matrix was prepared by a heat extrusion technique using a lyophilized powder of the protein and 11 different types of synthetic PGEFs, which varied in degree of glycerol polymerization (di- and tetra-), chain length of fatty acids (myristate, palmitate and stearate) and degree of fatty acid esterification (mono-, di- and tri-). In an in-vitro release study using an enzyme-linked immunosorbent assay (ELISA) as a detection method, the matrices prepared from a monoglyceride (used for comparison) and from diglycerol esters exhibited a biphasic release pattern with a large initial burst followed by slow release. In contrast, the matrices prepared from tetraglycerol esters showed a steady rate of release without a large initial burst. In an in vivo release study, initial bursts of IFN-alpha release were, also, dramatically reduced when the matrices were prepared from the tetraglycerol esters of palmitate and stearate, and the mean residence time (MRT) of IFN-alpha was prolonged, whereas the matrices prepared from monoglyceride and from diglycerol esters showed large initial bursts of IFN-alpha release. Since the release rates from the matrices prepared from the tetraglycerol esters of palmitate and stearate were governed by Jander's equation modified for a cylindrical matrix, the release from those matrices was concluded to be a diffusion-controlled process. The bioavailability of IFN-alpha after implantation of the matrix formulation prepared using all types of PGEFs, except for tetraglycerol triesters, was almost equivalent to that after injection of IFN-alpha solution; consequently, IFN-alpha in these matrices appears to remain stable during the release period.

Alpha-adrenoceptor blockade modifies neurally induced atrial arrhythmias.

PubMed

Richer, Louis-Philippe; Vinet, Alain; Kus, Teresa; Cardinal, René; Ardell, Jeffrey L; Armour, John Andrew

2008-10-01

Our objective was to determine whether neuronally induced atrial arrhythmias can be modified by alpha-adrenergic receptor blockade. In 30 anesthetized dogs, trains of five electrical stimuli (1 mA; 1 ms) were delivered immediately after the P wave of the ECG to mediastinal nerves associated with the superior vena cava. Regional atrial electrical events were monitored with 191 atrial unipolar electrodes. Mediastinal nerve sites were identified that reproducibly initiated atrial arrhythmias. These sites were then restimulated following 1 h (time control, n = 6), or the intravenous administration of naftopidil (alpha(1)-adrenergic blocker: 0.2 mg/kg, n = 6), yohimbine (alpha(2)-adrenergic blocker: 1 mg/kg, n = 6) or both (n = 8). A ganglionic blocker (hexamethonium: 1 mg/kg) was tested in four dogs. Stimulation of mediastinal nerves sites consistently elicited atrial tachyarrhythmias. Repeat stimulation after 1 h in the time-control group exerted a 19% decrease of the sites still able to induce atrial tachyarrhythmias. Hexamethonium inactivated 78% of the previously active sites. Combined alpha-adrenoceptor blockade inactivated 72% of the previously active sites. Bradycardia responses induced by mediastinal nerve stimulation were blunted by hexamethonium, but not by alpha(1,2)-adrenergic blockade. Naftopidil or yohimbine alone eliminated atrial arrhythmia induction from 31% and 34% of the sites (similar to time control). We conclude that heterogeneous activation of the intrinsic cardiac nervous system results in atrial arrhythmias that involve intrinsic cardiac neuronal alpha-adrenoceptors. In contrast to the global suppression exerted by hexamethonium, we conclude that alpha-adrenoceptor blockade targets intrinsic cardiac local circuit neurons involved in arrhythmia formation and not the flow-through efferent projections of the cardiac nervous system.

Alpha lipoic acid protects the heart against myocardial post ischemia-reperfusion arrhythmias via KATP channel activation in isolated rat hearts.

PubMed

Dudek, Magdalena; Knutelska, Joanna; Bednarski, Marek; Nowiński, Leszek; Zygmunt, Małgorzata; Bilska-Wilkosz, Anna; Iciek, Małgorzata; Otto, Monika; Żytka, Iwona; Sapa, Jacek; Włodek, Lidia; Filipek, Barbara

2014-06-01

The cardiovascular effects of alpha lipoic acid were evaluated in isolated rat hearts exposed to ischemia-reperfusion injury in vitro. Alpha-lipoic acid raised the level of sulfane sulfur playing an important role in the release of hydrogen sulfide. H2S was shown to prevent the post-reperfusion arrhythmias and to protect the cardiomyocytes from death caused by hypoxia. The activation of potassium ATP-sensitive channels (K(ATP) channels) is one of the most important mechanisms of action of hydrogen sulfide in the cardiovascular system. The aim of this study was to investigate whether alpha lipoic acid can prevent the occurrence of post-reperfusion arrhythmias in vitro using a Langendorff model of ischemia-reperfusion in rats affecting the K(ATP) channels. Alpha lipoic acid significantly improved post-reperfusion cardiac function (reducing incidence of arrhythmias), especially in a dose of 10(-7)M. These cardiovascular effects of this compound on the measured parameters were reversed by glibenclamide, a selective K(ATP) blocker. Alpha lipoic acid increased the level of sulfane sulfur in the hearts. This may suggest that the positive effects caused by alpha lipoic acid in the cardiovascular system are not only related to its strong antioxidant activity, and the influence on the activity of such enzymes as aldehyde dehydrogenase 2, as previously suggested, but this compound can affect K(ATP) channels. It is possible that this indirect effect of alpha lipoic acid is connected with changes in the release of sulfane sulfur and hydrogen sulfide. Copyright © 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

Expression and in vitro regulation of integrins by normal human urothelial cells.

PubMed

Southgate, J; Kennedy, W; Hutton, K A; Trejdosiewicz, L K

1995-08-01

Integrins are thought to be essential adhesion receptors for the maintenance of tissue histioarchitecture. The purpose of this study was to determine integrin expression patterns in the human stratified transitional epithelium of the urinary tract (urothelium). In situ expression patterns were compared with in vitro expression, using a normal cell culture model system in which the effects of cell stratification can be studied independently of differentiation. By immunohistological criteria, the urothelia of bladder, ureter and renal pelvis expressed alpha 2 beta 1 and alpha 3 beta 1 integrins in all layers at intercellular junctions, and cytoplasmically in the lower strata. By contrast, alpha 6 beta 4 and occasionally alpha v beta 4 were expressed only by basal cells and localised to the basal lamina. These expression patterns were unaltered in specimens where an inflammatory cell infiltrate was present. In long-term cultures of normal urothelial cells maintained in a low-Ca++ serum-free medium, the monolayer cultures expressed alpha 2 beta 1, alpha 3 beta 1 and alpha 5 beta 1 integrins at intercellular junctions and in cytoplasmic inclusions, whereas alpha 6 beta 4 was distributed in a random pattern over the substratum. Increasing exogenous Ca++ concentrations induced cell stratification and desmosome formation, but not cytodifferentiation. Under these conditions, alpha 6 beta 4 became cell-, rather than substratum-associated, localising particularly to filopodia and lamellipodia. Quantitation of integrin expression by flow cytometry confirmed increased surface expression of alpha 6 beta 4 in high Ca++ media, and also of alpha 3 and alpha 5, but not alpha 2, subunits. These results suggest that alpha 2 beta 1 and alpha 3 beta 1 integrins, although differentially regulated, are mainly involved in homotypic cell-cell interactions and the maintenance of a stratified morphology, whereas alpha 6 beta 4 is the principal integrin involved in substratum adhesion.

Renal alpha-smooth muscle actin: a new prognostic factor for lupus nephritis.

PubMed

Makni, Kaouthar; Jarraya, Faïçal; Khabir, Abdelmajid; Hentati, Basma; Hmida, Mohamed Ben; Makni, Hafedh; Boudawara, Tahia; Jlidi, Rchid; Hachicha, Jamil; Ayadi, Hammadi

2009-08-01

Systemic lupus erythematosus (SLE) is the prototype of autoimmune disease where renal involvement is frequent and always severe. Histological prognostic factors proposed for lupus nephritis (LN) including the World Health Organization and International Society of Nephrology/Renal Pathology Society--Working Group on the Classification classifications, active (AI) and chronicity (CI) indices may not predict response to treatment. The aim of this study was to correlate alpha-smooth muscle actin (alpha-SMA) expression, an early marker of glomerular and interstitial response to injury, to AI and CI, renal scarring progression and response to treatment. Fifty-seven kidney biopsy specimens obtained from 32 patients suffering from LN were studied. Twenty patients with class IV LN at first biopsy were identified to study renal progression to chronic renal failure until the use of immunosuppressive treatment. Interstitial alpha-SMA (I-alpha-SMA) was correlated only with CI (P < 0.001) whereas mesangial alpha-SMA (M-alpha-SMA) was correlated with neither LN activity (P = 0.126) nor sclerosis (P = 0.297). Only I-alpha-SMA was correlated with renal failure (P = 0.01). We divided patients with class IV LN into progressors and non-progressors based on the slope of serum creatinine. At first biopsy, M-alpha-SMA and I-alpha-SMA, but not AI and CI, were correlated with renal failure progression (M-alpha-SMA, 9.7b1.1 vs 7.8b1.4, P = 0.004; and I-alpha-SMA, 9.3b1.1 vs 6.5b3.2, P = 0.011). The study data highlight that I-alpha-SMA immunostain in class IV LN patients was correlated with chronicity indices. Moreover, M-alpha-SMA and I-alpha-SMA expression in first biopsy predicted renal progression modality. alpha-SMA expression may therefore be a useful marker to predict renal prognosis in LN.

A comparison of lidar inversion methods for cirrus applications

NASA Technical Reports Server (NTRS)

Elouragini, Salem; Flamant, Pierre H.

1992-01-01

Several methods for inverting the lidar equation are suggested as means to derive the cirrus optical properties (beta backscatter, alpha extinction coefficients, and delta optical depth) at one wavelength. The lidar equation can be inverted in a linear or logarithmic form; either solution assumes a linear relationship: beta = kappa(alpha), where kappa is the lidar ratio. A number of problems prevent us from calculating alpha (or beta) with a good accuracy. Some of these are as follows: (1) the multiple scattering effect (most authors neglect it); (2) an absolute calibration of the lidar system (difficult and sometimes not possible); (3) lack of accuracy on the lidar ratio k (taken as constant, but in fact it varies with range and cloud species); and (4) the determination of boundary condition for logarithmic solution which depends on signal to noise ration (SNR) at cloud top. An inversion in a linear form needs an absolute calibration of the system. In practice one uses molecular backscattering below the cloud to calibrate the system. This method is not permanent because the lower atmosphere turbidity is variable. For a logarithmic solution, a reference extinction coefficient (alpha(sub f)) at cloud top is required. Several methods to determine alpha(sub f) were suggested. We tested these methods at low SNR. This led us to propose two new methods referenced as S1 and S2.

A Faraday rotation search for magnetic fields in quasar damped Ly alpha absorption systems

NASA Technical Reports Server (NTRS)

Oren, Abraham L.; Wolfe, Arthur M.

1995-01-01

We present the results of a Faraday rotation survey of 61 radio-bright QSOs conducted at the National Radio Astronomy Observatory (NRAO) Very Large Array (VLA). The Galactic contribution to the Faraday rotation is estimated and subtracted to determine the extragalactic rotation measure (RRM) for each source. Eleven of these QSOs are known to exhibit damped Ly alpha absorption. The rate of incidence of significant Faraday rotation of these 11 sources is compared to the remaining 50 and is found to be higher at the 99.8% confidence level. However, as this is based upon only two detections of Faraday rotation in the damped Ly alpha sample, the result is only tentative. If the two detections in the damped Ly alpha sample are dug to the absorbing systems, then the inferred rotation measure induced by these systems is roughly 250 rad/sq m. The two detections were for the two lowest redshift absorbers in the sample. We find that a rotation measure of 250 rad/sq m would have gone undetected for any other absorber in the damped Ly alpha sample due to the 1/(1 + 2) squared dilution of the observed RRM with redshift. Thus the data are consistent with, but do not prove, the hypothesis that Faraday rotation is a generic property of damped Ly alpha absorbers. We do not confirm the suggestion that the amplitude of RRMs increases with redshift. Rather, the data are consistent with no redshift evolution. We find that the uncertainty in the estimation of the Galactic rotation measure (GRM) is a more serious problem than previously realized for extra-galactic Faraday rotation studies of QSO absorbers. A careful analysis of current methods for estimating GRM indicate that it can be determined to an accuracy of about 15 - 20 rad/sq m. Previous studies underestimated this uncertainty by more than a factor of 2. Due to this uncertainty, rotation measures such as we suspect are associated with damped Ly alpha absorption systems can only be detected at redshifts less than z approximately equal 1.

Identification of a GTP-binding protein. cap alpha. subunit that lacks an apparent ADP-ribosylation site for pertussis toxin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fong, H.K.W.; Yoshimoto, K.K.; Eversole-Cire, P.

1988-05-01

Recent molecular cloning of cDNA for the ..cap alpha.. subunit of bovine transducin (a guanine nucleotide-binding regulatory protein, or G protein) has revealed the presence of two retinal-specific transducins, called T/sub r/ and T/sub c/, which are expressed in rod or cone photoreceptor cells. In a further study of G-protein diversity and signal transduction in the retina, the authors have identified a G-protein ..cap alpha.. subunit, which they refer to as G/sub z/..cap alpha.., by isolating a human retinal cDNA clone that cross-hybridizes at reduced stringency with bovine T/sub r/ ..cap alpha..-subunit cDNA. The deduced amino acid sequence of G/submore » z/..cap alpha.. is 41-67% identical with those of other known G-protein ..cap alpha.. subunits. However, the 355-residue G/sub z/..cap alpha.. lacks a consensus site for ADP-ribosylation by pertussis toxin, and its amino acid sequence varies within a number of regions that are strongly conserved among all of the other G-protein ..cap alpha.. subunits. They suggest that G/sub z/..cap alpha.., which appears to be highly expressed in neural tissues, represents a member of a subfamily of G proteins that mediate signal transduction in pertussis toxin-insensitive systems.« less

TNF-alpha suppresses the expression of clock genes by interfering with E-box-mediated transcription.

PubMed

Cavadini, Gionata; Petrzilka, Saskia; Kohler, Philipp; Jud, Corinne; Tobler, Irene; Birchler, Thomas; Fontana, Adriano

2007-07-31

Production of TNF-alpha and IL-1 in infectious and autoimmune diseases is associated with fever, fatigue, and sleep disturbances, which are collectively referred to as sickness behavior syndrome. In mice TNF-alpha and IL-1 increase nonrapid eye movement sleep. Because clock genes regulate the circadian rhythm and thereby locomotor activity and may alter sleep architecture we assessed the influence of TNF-alpha on the circadian timing system. TNF-alpha is shown here to suppress the expression of the PAR bZip clock-controlled genes Dbp, Tef, and Hlf and of the period genes Per1, Per2, and Per3 in fibroblasts in vitro and in vivo in the liver of mice infused with the cytokine. The effect of TNF-alpha on clock genes is shared by IL-1beta, but not by IFN-alpha, and IL-6. Furthermore, TNF-alpha interferes with the expression of Dbp in the suprachiasmatic nucleus and causes prolonged rest periods in the dark when mice show spontaneous locomotor activity. Using clock reporter genes TNF-alpha is found here to inhibit CLOCK-BMAL1-induced activation of E-box regulatory elements-dependent clock gene promoters. We suggest that the increase of TNF-alpha and IL-1beta, as seen in infectious and autoimmune diseases, impairs clock gene functions and causes fatigue.

The IL-6/sIL-6R treatment of a malignant melanoma cell line enhances susceptibility to TNF-{alpha}-induced apoptosis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wagley, Yadav; Yoo, Yung-Choon; Seo, Han Geuk

2007-03-23

Melanoma is an intractable tumor that has shown very impressive and promising response to local administration of high dose recombinant TNF-{alpha} in combination with IFN-{gamma} in clinical studies. In this study, we investigated the effect of IL-6/sIL-6R on TNF-{alpha}-resistant B16/F10.9 melanoma cells. A low dose of TNF-{alpha} or IL-6/sIL-6R had minimal affect on the cell growth. However, the highly active fusion protein of sIL-6R and IL-6 (IL6RIL6), covalently linked by a flexible peptide, sensitized TNF-{alpha}-resistant F10.9 melanoma cells to TNF-{alpha}-induced apoptosis. Stimulation of the cells with IL6RIL6 plus TNF-{alpha} resulted in both the activation of caspase-3 and the reduction ofmore » bcl-2 expression. Flow cytometry analysis showed that IL6RIL6-upregulated TNF-R55 and TNF-R75 expression, suggesting an increase in TNF-{alpha} responsiveness by IL6RIL6 resulting from the induction of TNF receptors. Moreover, exposure of F10.9 cells to neutralizing antibody to TNF-R55 significantly inhibited IL6RIL6/TNF-{alpha}-induced cytotoxicity. These results suggest that the IL6/sIL6R/gp130 system, which sensitizes TNF-{alpha}-resistant melanoma cells to TNF-{alpha}-induced apoptosis, may provide a new target for immunotherapy.« less

Development of ultrahigh resolution alpha particle imaging detector using 1 mm channel size Si-PM array

NASA Astrophysics Data System (ADS)

Yamamoto, Seiichi; Kawaguchi, Wataru

2018-06-01

For precise distribution measurements of alpha particles, a high-resolution alpha particle imaging detector is required. Although combining a thin scintillator with a silicon photomultiplier (Si-PM) array is a promising method for achieving high resolution, the spatial resolution is limited. Reducing the size of the Si-PM array is a possible approach to improving the spatial resolution of the alpha particle imaging detector. Consequently, we employed a 1 mm channel size Si-PM array combined with a thin ZnS(Ag) sheet to form an alpha particle imaging detector and evaluated the performance. For the developed alpha particle imaging detector, an Si-PM array with 1 mm x 1 mm channel size arranged 8 x 8 was optically coupled to a ZnS(Ag) sheet with a 1-mm-thick light guide between them. The size of the alpha particle imaging detector was 9.5 mm x 9.5 mm. The spatial resolution of the developed alpha particle imaging detector was 0.14 mm FWHM, and the energy resolution was 74% FWHM for 5.5 MeV alpha particles. The uniformity of the imaging detector at the central part of the field of view (FOV) was ±4.7%. The background count rate was 0.06 counts/min. We obtained various high-resolution phantom images for alpha particles with the developed system. We conclude that the developed imaging detector is promising for high-resolution distribution measurements of alpha particles.

On the limit of field-aligned current intensity in the polar magnetosphere

NASA Technical Reports Server (NTRS)

Cole, Keith D.

1991-01-01

Field-aligned current (FAC) is here defined by 4 pi j = alpha B, where alpha is constant along a magnetic field line. The upper limit value of alpha in the polar magnetosphere, possible source regions of the strongest FAC and the relationship of them to some auroral and ionospheric irregularity cross-field scale sizes are discussed. Cross-field dimensions of the strongest FAC are related to the gyroradii of source particles (O(+), He(2+), He(+), H(+), e) in the current-generating region. It is suggested that experimental determination, and mapping of the values of alpha, may assist with the search for the generators of such currents in near-earth space including in the nearby solar wind. The upper limit of alpha is associated with the breakup of FAC systems.

Behavioural characterisation of the alpha-mannosidosis guinea pig.

PubMed

Robinson, A J; Crawley, A C; Auclair, D; Weston, P F; Hirte, C; Hemsley, K M; Hopwood, J J

2008-01-25

alpha-Mannosidosis is a lysosomal storage disorder resulting from a functional deficiency of the lysosomal enzyme alpha-mannosidase. This deficiency results in the accumulation of various oligosaccharides in the lysosomes of affected individuals, causing somatic pathology and progressive neurological degeneration that results in cognitive deficits, ataxia, and other neurological symptoms. We have a naturally occurring guinea pig model of this disease which exhibits a deficiency of lysosomal alpha-mannosidase and has a similar clinical presentation to human alpha-mannosidosis. Various tests were developed in the present study to characterise and quantitate the loss of neurological function in alpha-mannosidosis guinea pigs and to follow closely the progression of the disease. General neurological examinations showed progressive differences in alpha-mannosidosis animals from approximately 1 month of age. Significant differences were observed in hind limb gait width from 2 months of age and significant cognitive (memory and learning) deficits were observed from 3 months of age. Evoked response tests showed an increase in somatosensory P1 peak latency in alpha-mannosidosis guinea pigs from approximately 2 months of age, as well as progressive hearing loss using auditory brainstem evoked responses. The alpha-mannosidosis guinea pig therefore appears to exhibit many of the characteristics of the human disease, and will be useful in evaluating therapies for treatment of central nervous system pathology.

Rat epileptic seizures evoked by BmK {alpha}IV and its possible mechanisms involved in sodium channels

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chai Zhifang; Bai Zhantao; Zhang Xuying

2007-05-01

This study showed that rat unilateral intracerebroventricular injection of BmK {alpha}IV, a sodium channel modulator derived from scorpion Buthus martensi Karsch, induced clusters of spikes, epileptic discharges and convulsion-related behavioral changes. BmK {alpha}IV potently promoted the release of endogenous glutamate from rat cerebrocortical synaptosomes. In vitro examination of the effect of BmK {alpha}IV on intrasynaptosomal free calcium concentration [Ca{sup 2+}]{sub i} and sodium concentration [Na{sup +}]{sub i} revealed that BmK {alpha}IV-evoked glutamate release from synaptosomes was associated with an increase in Ca{sup 2+} and Na{sup +} influx. Moreover, BmK {alpha}IV-mediated glutamate release and ion influx was completely blocked by tetrodotoxin,more » a blocker of sodium channel. Together, these results suggest that the induction of BmK {alpha}IV-evoked epileptic seizures may be involved in the modulation of BmK {alpha}IV on tetrodotoxin-sensitive sodium channels located on the nerve terminal, which subsequently enhances the Ca{sup 2+} influx to cause an increase of glutamate release. These findings may provide some insight regarding the mechanism of neuronal action of BmK {alpha}IV in the central nervous system for understanding epileptogenesis involved in sodium channels.« less

Instrument performance of a radon measuring system with the alpha-track detection technique.

PubMed

Tokonami, S; Zhuo, W; Ryuo, H; Yonehara, H; Yamada, Y; Shimo, M

2003-01-01

An instrument performance test has been carried out for a radon measuring system made in Hungary. The system measures radon using the alpha-track detection technique. It consists of three parts: the passive detector, the etching unit and the evaluation unit. A CR-39 detector is used as the radiation detector. Alpha-track reading and data analysis are carried out after chemical etching. The following subjects were examined in the present study: (1) radon sensitivity, (2) performance of etching and evaluation processes and (3) thoron sensitivity. The radon sensitivity of 6.9 x 10(-4) mm(-2) (Bq m(-3) d)(-1) was acceptable for practical application. The thoron sensitivity was estimated to be as low as 3.3 x 10(-5) mm(-2) (Bq m(-3) d)(-1) from the experimental study.

Two subunits of the 55 K porcine zona pellucida glycoprotein family are immunologically distinct

DOE Office of Scientific and Technical Information (OSTI.GOV)

Subramanian, M.G.; Yurewicz, E.C.; Sacco, A.G.

1986-03-01

The 55K glycoprotein family (ZP3) of the porcine zona pellucida is comprised of two subunits of 46 K and 45 K which can be resolved by endo-..beta..-galactosidase digestion of ZP3 followed by reversed phase HPLC on Vydac C4 resin. Gel electrophoresis revealed that the 46 K component (EBDG..cap alpha..) is approx. 95% pure and the 45 K component (EBGD..beta..) is 100% pure. In the present study, these two subunits were evaluated immunologically by RIA. Under similar reaction protocols (chloramine-T iodination procedure) comparable specific activities were obtained for EBGD..cap alpha.. (33.06 +/- 7.5 ..mu..ci/..mu..gm), EBGD..beta.. (30.45 +/- 1.6) and ZP3 (26.3more » +/- 1.3). Antibody (Ab) titration studies revealed that EBGD..cap alpha.. and ..beta.. are potent immunogens and /sup 125/I-EBGD..cap alpha.. showed minimal cross reactivity to EBGD..beta..-Ab (8% bound at 1:500 dilution), whereas, /sup 125/I-EBGD..beta.. showed a greater degree of cross reactivity to EBGD..cap alpha..-Ab (23% bound at 1:500 dilution). Maximum binding for the two labeled antigens against homologous Abs (1:500) was > 60%. Dose response studies revealed that in the /sup 125/I-EBGD..cap alpha.. vs EBGD..cap alpha.. -Ab system, the 50% intercept was 3.25 +/- 0.32 ng for EBGD..cap alpha.. and 472.43 +/- 30.26 ng for EBGD..beta.. (p < 0.01), whereas, in the /sup 125/I-EBGD..beta.. vs EBGD..beta..-Ab system the 50% intercept was 3.51 +/- 0.58 for EBGD..beta.. and 166.77 +/- 49.20 for EBGD..cap alpha.. (p < 0.01). No significant differences were observed in the slopes of the dose response curves. It is concluded that the two subunits of ZP3 possess distinct immunologic characteristics as evaluated by RIA.« less

Characterization of alpha-ketobutyrate metabolism in rat tissues: effects of dietary protein and fasting.

PubMed

Steele, R D; Weber, H; Patterson, J I

1984-04-01

The oxidative decarboxylation of alpha-ketobutyrate was studied in rat tissue preparations. Decarboxylation was confined to the mitochondrial fraction and required coenzyme A, NAD, TPP and FAD for optimal activity in solubilized preparations. The pH optimum for this reaction in liver was 7.8, somewhat higher than that reported for other alpha-keto acid dehydrogenases. An apparent Km of 0.63 mM for alpha-ketobutyrate was determined for the rat liver system. Competition by other alpha-keto acids at 10 mM concentrations inhibited enzyme activity up to 75%. Tissue distribution of alpha-ketobutyrate dehydrogenase activity relative to liver activity was (in percent): liver, 100; heart, 127; brain, 63; kidney, 57; skeletal muscle, 38; and small intestine, 7. Total liver alpha-ketobutyrate dehydrogenase was decreased by 40% after a 24-hour fast. Similar results were found for kidney and heart activity. alpha-Aminobutyrate-pyruvate aminotransferase activity in liver or kidney was not affected by fasting; however, it was induced in liver by 50% after feeding a 40% casein diet for 10 days compared to rats fed a 20% casein diet. Increasing the dietary casein content from 6 through 40% of the diet resulted in about a fivefold increase in liver alpha-ketobutyrate dehydrogenase activity. The substantial extrahepatic capacity for alpha-ketobutyrate metabolism makes it unlikely that a loss of liver function results in an inability to metabolize alpha-ketobutyrate. Whether alpha-ketobutyrate is decarboxylated by a specific enzyme or by an already characterized complex such as pyruvate dehydrogenase or the branched-chain keto acid dehydrogenase remains to be established.

A chemometric approach for optimizing protein covalent immobilization on magnetic core-shell nanoparticles in view of an alternative immunoassay.

PubMed

Teste, Bruno; Vial, Jérôme; Descroix, Stéphanie; Georgelin, Thomas; Siaugue, Jean-Michel; Petr, Jan; Varenne, Anne; Hennion, Marie-Claire

2010-06-15

A chemometric approach was developed to optimize the grafting of a bovine milk allergen: alpha-Lactalbumin (alpha-Lac) on colloidal functionalized magnetic core-shell nanoparticles (MCSNP). Such nanoparticles, functionalized with polyethyleneglycol and amino groups, exhibit a 30nm physical diameter and behave as a quasi-homogeneous system. The alpha-Lac immobilization was achieved through the covalent binding between MCSNP amino groups and alpha-Lac carboxylic moieties using the well-known tandem carbodiimide (EDC) and hydroxysulfosuccinimide (NHS). In this study, a chemometric approach was employed to highlight the parameters influencing the number of grafted proteins on the MCSNP. Three factors were evaluated: the ratio in concentration between EDC and alpha-Lac, between NHS and EDC and the concentration of alpha-Lac. After a first full factorial design to delimit the region of the space where the optimum could be located, a central composite design was then carried out to predict the best grafting conditions. It was established and experimentally confirmed that the optimum parameters are [EDC]/[alpha-Lac]=25; [NHS]/[EDC]=1.55 and alpha-Lac=24.85nmolmL(-1). In these optimal conditions, MCSNP surface was successfully saturated with alpha-Lac (34 alpha-Lac/MCSNP) with a high reproducibility (RSD=2%). The colloidal stability of MCSNP grafted with alpha-Lac as well as the immunological interactions using anti alpha-Lac antibody were then investigated in different buffers. The results emphasized that a 50mM MES buffer (pH 6) allows an efficient immune capture and a satisfying colloidal stability which provide an immunological interaction in homogeneous liquid phase.

Elevation of CSF tumor necrosis factor alpha levels in new daily persistent headache and treatment refractory chronic migraine.

PubMed

Rozen, Todd; Swidan, Sahar Z

2007-01-01

To determine if patients with new daily persistent headache (NDPH) have elevated levels of tumor necrosis factor alpha (TNF alpha) in the CSF. NDPH is considered one of the most treatment resistant of all headache syndromes. This reflects a lack of understanding of its pathogenesis. As a certain percentage of NDPH patients have their headaches start after an infection, the possibility of a persistent state of systemic or CNS inflammation comes into question. TNF alpha is a proinflammatory cytokine involved in brain immune and inflammatory activities, as well as in pain initiation. The goal of this study was to look at TNF alpha levels in the CSF of NDPH patients, to determine if CNS inflammation may play some role in the pathogenesis of this condition. CSF TNF alpha levels were studied in 38 patients: 20 with NDPH and a control population of 16 patients with chronic migraine (CM), and 2 with post-traumatic headache (PT). CSF TNF alpha levels were elevated in 19 of 20 NDPH patients, 16 of 16 CM patients, and both PT patients. Serum TNF alpha levels were normal in most of the study subjects. An elevation of CSF TNF alpha levels was found in almost all NDPH patients and suggest a role for TNF alpha in the pathogenesis of this condition. Surprisingly, all CM and PT patients tested had elevated CSF TNF alpha levels. In most patients with elevated CSF levels, serum TNF alpha levels were normal. All of these syndromes may be manifestations of CNS inflammation. As most of the positive-tested patients showed minimal to no improvement during aggressive inpatient treatment, persistent elevation of CSF TNF alpha levels may be one of the causes of treatment refractory CDH.

CPT1{alpha} over-expression increases long-chain fatty acid oxidation and reduces cell viability with incremental palmitic acid concentration in 293T cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jambor de Sousa, Ulrike L.; Koss, Michael D.; Fillies, Marion

2005-12-16

To test the cellular response to an increased fatty acid oxidation, we generated a vector for an inducible expression of the rate-limiting enzyme carnitine palmitoyl-transferase 1{alpha} (CPT1{alpha}). Human embryonic 293T kidney cells were transiently transfected and expression of the CPT1{alpha} transgene in the tet-on vector was activated with doxycycline. Fatty acid oxidation was measured by determining the conversion of supplemented, synthetic cis-10-heptadecenoic acid (C17:1n-7) to C15:ln-7. CPT1{alpha} over-expression increased mitochondrial long-chain fatty acid oxidation about 6-fold. Addition of palmitic acid (PA) decreased viability of CPT1{alpha} over-expressing cells in a concentration-dependent manner. Both, PA and CPT1{alpha} over-expression increased cell death. Interestingly,more » PA reduced total cell number only in cells over-expressing CPT1{alpha}, suggesting an effect on cell proliferation that requires PA translocation across the mitochondrial inner membrane. This inducible expression system should be well suited to study the roles of CPT1 and fatty acid oxidation in lipotoxicity and metabolism in vivo.« less

Influence of thyroid status on hepatic alpha 1-adrenoreceptor responsiveness.

PubMed

Daza, F J; Parrilla, R; Martín-Requero, A

1997-12-01

The present work aimed to elucidate the influence of thyroid functional status on the alpha 1-adrenoreceptor-induced activation of hepatic metabolic functions. The experiments were performed in either a nonrecirculating liver perfusion system featuring continuous monitoring of portal pressure, PO2, pCa, and pH, or isolated hepatocytes from euthyroid, hyperthyroid, and hypothyroid rats. Hypothyroidism decreased the alpha 1-adrenergic stimulation of respiration, glycogen breakdown, and gluconeogenesis. These effects were accompanied by a decreased intracellular Ca2+ mobilization corroborating that those processes are regulated by the Ca(2+)-dependent branch of the alpha 1-adrenoreceptor signaling pathway. Moreover, in hyperthyroid rats the alpha 1-adrenergic-induced increase in cytosolic Ca2+ was enhanced, and glucose synthesis or mobilization was not altered. The thyroid status influenced neither the alpha 1-adrenergic stimulation of vascular smooth muscle contraction nor the alpha 1-agonist-induced intracellular alkalinization and protein kinase C (PKC) activation. Thus the distinct impairment of the Ca(2+)-dependent branch of the alpha 1-adrenoreceptor signaling pathway by thyroid status provides a useful tool to investigate the role played by each signaling pathway, Ca2+ or PKC, in controlling hepatic functions.

Extremely-efficient, miniaturized, long-lived alpha-voltaic power source using liquid gallium

NASA Technical Reports Server (NTRS)

Snyder, G. Jeffrey (Inventor); Patel, Jagdishbhai (Inventor); Fleurial, Jean-Pierre (Inventor)

2004-01-01

A power source converts .alpha.-particle energy to electricity for use in electrical systems. Liquid gallium or other liquid medium is subjected to .alpha.-particle emissions. Electrons are freed by collision from neutral gallium atoms to provide gallium ions. The electrons migrate to a cathode while the gallium ions migrate to an anode. A current and/or voltage difference then arises between the cathode and anode because of the work function difference of the cathode and anode. Gallium atoms are regenerated by the receiving of electrons from the anode enabling the generation of additional electrons from additional .alpha.-particle collisions.

3-Arylpiperazinylethyl-1H-pyrrolo[2,3-d]pyrimidine-2,4(3H,7H)-dione derivatives as novel, high-affinity and selective alpha(1)-adrenoceptor ligands.

PubMed

Pittalà, Valeria; Romeo, Giuseppe; Salerno, Loredana; Siracusa, Maria Angela; Modica, Maria; Materia, Luisa; Mereghetti, Ilario; Cagnotto, Alfredo; Mennini, Tiziana; Marucci, Gabriella; Angeli, Piero; Russo, Filippo

2006-01-01

The discovery of a new series of selective and high-affinity alpha(1)-adrenoceptor (alpha(1)-AR) ligands, characterized by a 1H-pyrrolo[2,3-d]-pyrimidine-2,4(3H,7H)-dione system, is described in this paper. Some synthesized compounds, including 20, 22, and 30, displayed affinity in the nanomolar range for alpha(1)-ARs and substantial selectivity with respect to 5-HT(1A) and dopaminergic D(1) and D(2) receptors. Functional assays, performed on selected derivatives, showed antagonistic properties.

Thermodynamics of alpha-Cyclodextrin-p-Nitrophenyl Glycoside Complexes. A Simple System To Understand the Energetics of Carbohydrate Interactions in Water.

PubMed

Junquera, Elena; Laynez, José; Menéndez, Margarita; Sharma, Sunil; Penadés, Soledad

1996-10-04

Thermodynamic studies of the binding of a series of p-nitrophenyl glycosides (PNPGly) of varying stereochemistry to alpha-cyclodextrin (alpha-CD) were performed at three different temperatures (25, 35, and 42 degrees C) using a microcalorimetric technique. The system p-nitrophenol (PNP) at pH = 3 and alpha-CD was also studied for the sake of comparison. All these complexes were found to be enthalpy driven with a favorable enthalpic term clearly dominant over an unfavorable entropic term. A clear enthalpy-entropy compensation effect was observed at all the temperatures, with a slope close to unity (alpha = 1.02) and an intercept TDeltaS degrees (o) = 2.91 kcal mol(-)(1). This thermodynamic pattern is in agreement with those usually found for lectin-carbohydrate associations and for the binding processes of several host-guest systems. This pattern is explained in terms of the contribution of primarily two driving forces: the van der Waals interactions between the host and the guest, and the solvation/desolvation processes which accompany the association reaction. The presence of the carbohydrate molecule in the PNP ring causes a slight destabilization of the complex at 25 degrees C with respect to the alpha-CD-PNP (pH = 3) complex, although a different behavior has been observed depending on the axial/equatorial configuration of the glycoside and the temperature. This behavior is modulated by the stereochemistry of the glycoside. Differences were observed between the deoxy-derivatives (LAra and LFuc) and those derivatives with a hydroxymethyl group (Glc, Gal, Man). DeltaC(p) degrees values were obtained from the dependency of DeltaH degrees on temperature (=( partial differentialDeltaH degrees / partial differentialT)(p)). These values are small and negative except for alphaMan complex. For the latter complex, discrepancy between the calorimetric and the calculated van't Hoff enthalpies was observed. Parallels are drawn between the thermodynamics of our model and those proposed for carbohydrate-protein associations.

Absence of Fc epsilonRI alpha chain results in upregulation of Fc gammaRIII-dependent mast cell degranulation and anaphylaxis. Evidence of competition between Fc epsilonRI and Fc gammaRIII for limiting amounts of FcR beta and gamma chains.

PubMed Central

Dombrowicz, D; Flamand, V; Miyajima, I; Ravetch, J V; Galli, S J; Kinet, J P

1997-01-01

In mouse mast cells, both Fc epsilonRI and Fc gammaRIII are alpha beta gamma2 tetrameric complexes in which different alpha chains confer IgE or IgG ligand recognition while the signaling FcR beta and gamma chains are identical. We used primarily noninvasive techniques (changes in body temperature, dye extravasation) to assess systemic anaphylactic responses in nonanesthetized wild-type, Fc epsilonRI alpha chain -/- and FcR gamma chain -/- mice. We confirm that systemic anaphylaxis in mice can be mediated largely through IgG1 and Fc gammaRIII and we provide direct evidence that these responses reflect activation of Fc gammaRIII rather than Fc gammaRI. Furthermore, we show that Fc gammaRIII-dependent responses are more intense in normal than in congenic mast cell-deficient KitW/KitW-v mice, indicating that Fc gammaRIII responses have mast cell-dependent and -independent components. Finally, we demonstrate that the upregulation of cell surface expression of Fc gammaRIII seen in Fc epsilonRI alpha chain -/- mice corresponds to an increased association of Fc gammaRIII alpha chains with FcR beta and gamma chains and is associated with enhanced Fc gammaRIII-dependent mast cell degranulation and systemic anaphylactic responses. Therefore, the phenotype of the Fc epsilonRI alpha chain -/- mice suggests that expression of Fc epsilonRI and Fc gammaRIII is limited by availability of the FcR beta and gamma chains and that, in normal mice, changes in the expression of one receptor (Fc epsilonRI) may influence the expression of functional responses dependent on the other (Fc gammaRIII). PMID:9062349

Activation of the serotonergic system by pedaling exercise changes anterior cingulate cortex activity and improves negative emotion.

PubMed

Ohmatsu, Satoko; Nakano, Hideki; Tominaga, Takanori; Terakawa, Yuzo; Murata, Takaho; Morioka, Shu

2014-08-15

Pedaling exercise (PE) of moderate intensity has been shown to ease anxiety and discomfort; however, little is known of the changes that occur in brain activities and in the serotonergic (5-HT) system after PE. Therefore, this study was conducted for the following reasons: (1) to localize the changes in the brain activities induced by PE using a distributed source localization algorithm, (2) to examine the changes in frontal asymmetry, as used in the Davidson model, with electroencephalography (EEG) activity, and (3) to examine the effect of PE on the 5-HT system. A 32-channel EEG was used to record before and after PE. Profile of Mood States tests indicated that there was a significant decrease in tension-anxiety and a significant increase in vigor after PE. A standardized low-resolution brain electromagnetic tomography analysis showed a significant decrease in brain activities after PE in the alpha-2 band (10-12.5 Hz) in the anterior cingulate cortex (ACC). Moreover, a significant increase in frontal EEG asymmetry was observed after PE in the alpha-1 band (7.5-10 Hz). Urine 5-HT levels significantly increased after PE. Urine 5-HT levels positively correlated with the degree of frontal EEG asymmetry in the alpha-1 band and negatively correlated with brain activity in ACC. Our results suggested that PE activates the 5-HT system and consequently induces increases in frontal EEG asymmetry in the alpha-1 band and reductions of brain activity in the alpha-2 band in the ACC region. Copyright © 2014 Elsevier B.V. All rights reserved.

Performance of two-layer thermal barrier systems on directionally solidified Ni-Al-Mo and comparative effects of alloy thermal expansion on system life

NASA Technical Reports Server (NTRS)

Stecura, S.

1980-01-01

A promising two-layer thermal barrier coating system (TBS), Ni-16.4Cr-5.1A1-0.15Y/ZrO2-6.1Y2O3 (all in weight percent), was identified for directionally solidified Ni-Al-Mo (gamma/gamma' alpha). In cyclic furnace tests at 1095 C this system on gamma/gamma' alpha was better than Ni-16. 4Cr-5.1Al-0.15Y/ZrO2-7.8Y2O3 by about 50 percent. In natural gas - oxygen torch rig tests at 1250 C the ZrO2-6.1Y2O3 coating was better than the ZrO2-7.8Y2O3 coating by 95 percent, on MAR-M509 substrates and by 60 percent on gamma/gamma' alpha substrates. Decreasing the coefficient of thermal expansion of the substrate material from 17-18x10 to the -6 power/C (MAR-M200 + Hf and MAR-M509) to 11x10 to the -6 power/C (gamma/gamma' alpha) also resulted in improved TBS life. For example, in natural gas - oxygen torch rig tests at 1250 C, the life of Ni-16.4Cr-5.1Al-0.15Y/ZrO26.1Y2O3 was about 30 percent better on gamma/gamma' alpha than on MAR-M509 substrates. Thus compositional changes in the bond and thermal barrier coatings were shown to have a greater effect on TBS life than does the coefficient of thermal expansion.

[New concepts on the role of cytokines in the central nervous system].

PubMed

Jacque, C; Tchélingérian, J L

1994-11-01

Initially described as modulatory molecules in the peripheral immune system and during haematopoiesis, several cytokines also play a role in the brain. Their synthesis in the central nervous system (CNS) is not due solely to glial cell activation or invading immune cells. On the one hand, several functions of central neurons are modulated by cytokines such as IL-1, TNF alpha, IL-2 and IL-6. Thus, IL-1 and TNF alpha modulate the synthesis of several neuromediators and modify ion influxes. IL-2 regulates the effects of central dopaminergic neurons on cholinergic, noradrenergic, serotoninergic and glutamatergic functions. On the other hand, neurons have recently been shown to be able to synthesize some of these cytokines under specific traumatic conditions. For example, a lesion to the hippocampus induces neuronal synthesis of IL-1 alpha and TNF alpha. This induction through neuronal circuits may operate at a distance in contrast to the glial reaction operating only locally. The recent demonstration of the expression by central neurons of receptors specific for these cytokines support a potentially crucial role for these molecules in brain function. Some data emerge in the literature demonstrating a potent expression of cytokines in the central nervous system in numerous pathological situations. Then, it appears that, at the interface between nervous and immune systems, cytokines may bear a pivotal role in the development of specific symptoms in neuroimmune diseases.

Effect of Prazosin and Naltrexone on Script Induced Alcohol Craving in Veterans with Alcohol Use Disorders with and without Co-occurring PTSD

DTIC Science & Technology

2015-01-01

status, moderate medication response. 15. SUBJECT TERMS Alcohol Drinking, Drinking Behavior, Naltrexone, Prazosin, Adrenergic Agents, Adrenergice ...primates and humans express α1 adrenergic receptors. Given the interplay of the noradrenergic system with craving-related brain systems, blocking α1...Antagonists, Adrenergic alpha-1 receptor antagonists, Adrenergic alpha- antagonists, Antihypertensive agents, Narcotic antagonists, Therapeutic uses

Detection of Lyman-alpha emission from the Saturnian disk and from the ring system

NASA Technical Reports Server (NTRS)

Weiser, H.; Vitz, R. C.; Moos, H. W.

1977-01-01

A rocket-borne spectrograph detected H I Lyman-alpha emission from the disk of Saturn and from the vicinity of the planet. The signal is consistent with an emission brightness of 700 rayleighs for the disk and 200 rayleighs for the vicinity of Saturn. The emission from the vicinity of the planet may be due to a hydrogen atmosphere associated with the Saturnian ring system.

SHH-dependent knockout of HIF-1 alpha accelerates the degenerative process in mouse intervertebral disc.

PubMed

Wu, W J; Zhang, X K; Zheng, X F; Yang, Y H; Jiang, S D; Jiang, L S

2013-01-01

Hypoxia-inducible factor-1alpha (HIF-1 alpha) has been reported to have an important role in the metabolism and synthesis of extracellular matrix of the nucleus pulposus cells (NPCs) and was assumed to be involved in the process of intervertebral disc degeneration. The objective of this study was to investigate the role of HIF-1alpha in disc degeneration in vivo using a conditional HIF-1alpha knockout (KO) mouse model. ShhCre transgenic mice were mated with HIF-1 alpha fl/fl mice to generate conditional HIF-1alpha KO mice (HIF-1alpha fl/fl-ShhCre+). Three mice of each genotype (Wide-type and HIF-1alpha KO) at the age of 3 days, 6, and 12 weeks were sacrificed after genotyping. Five lumbar disc samples were harvested from each mouse, with a total of 45 disc samples for each genotype. In situ hybridization and immunohistochemical analysis were used to check the efficacy of HIF-1alpha knockout. Histological grading of the disc degeneration was performed according to the classification system proposed by Boos et al. Picro-sirius red staining, Safranine O/fast green staining and immunohistochemical study were used to evaluate the expression of aggrecan, type-II collagen and vascular endothelial growth factor (VEGF). Histologic analysis revealed more NPC deaths and signs of degeneration in HIF-1alpha KO mice and the degeneration scores of HIF-1alpha KO mice were significantly higher than those of the Wide-type mice at the age of 6 weeks and 12 weeks. There were less expressions of aggrecan, type-II collagen and VEGF in the intervertebral discs of HIF1-alpha KO mice than in those of wild-type mice. Taken together, the results of our study indicated that HIF-1alpha is a pivotal contributor to NPC survival and the homeotasis of extracellular matrix through the HIF-1alpha/VEGF signaling pathway, and plays an important role in the development of disc degeneration.

How dusty is α Centauri?. Excess or non-excess over the infrared photospheres of main-sequence stars

NASA Astrophysics Data System (ADS)

Wiegert, J.; Liseau, R.; Thébault, P.; Olofsson, G.; Mora, A.; Bryden, G.; Marshall, J. P.; Eiroa, C.; Montesinos, B.; Ardila, D.; Augereau, J. C.; Bayo Aran, A.; Danchi, W. C.; del Burgo, C.; Ertel, S.; Fridlund, M. C. W.; Hajigholi, M.; Krivov, A. V.; Pilbratt, G. L.; Roberge, A.; White, G. J.; Wolf, S.

2014-03-01

Context. Debris discs around main-sequence stars indicate the presence of larger rocky bodies. The components of the nearby, solar-type binary α Centauri have metallicities that are higher than solar, which is thought to promote giant planet formation. Aims: We aim to determine the level of emission from debris around the stars in the α Cen system. This requires knowledge of their photospheres. Having already detected the temperature minimum, Tmin, of α Cen A at far-infrared wavelengths, we here attempt to do the same for the more active companion α Cen B. Using the α Cen stars as templates, we study the possible effects that Tmin may have on the detectability of unresolved dust discs around other stars. Methods: We used Herschel-PACS, Herschel-SPIRE, and APEX-LABOCA photometry to determine the stellar spectral energy distributions in the far infrared and submillimetre. In addition, we used APEX-SHeFI observations for spectral line mapping to study the complex background around α Cen seen in the photometric images. Models of stellar atmospheres and of particulate discs, based on particle simulations and in conjunction with radiative transfer calculations, were used to estimate the amount of debris around these stars. Results: For solar-type stars more distant than α Cen, a fractional dust luminosity fd ≡ Ldust/Lstar 2 × 10-7 could account for SEDs that do not exhibit the Tmin effect. This is comparable to estimates of fd for the Edgeworth-Kuiper belt of the solar system. In contrast to the far infrared, slight excesses at the 2.5σ level are observed at 24 μm for both α Cen A and B, which, if interpreted as due to zodiacal-type dust emission, would correspond to fd (1-3) × 10-5, i.e. some 102 times that of the local zodiacal cloud. Assuming simple power-law size distributions of the dust grains, dynamical disc modelling leads to rough mass estimates of the putative Zodi belts around the α Cen stars, viz. ≲4 × 10-6 M≤ftmoon of 4 to 1000 μm size grains, distributed according to n(a) ∝ a-3.5. Similarly, for filled-in Tmin emission, corresponding Edgeworth-Kuiper belts could account for {˜ 10-3 M≤ftmoon} of dust. Conclusions: Our far-infrared observations lead to estimates of upper limits to the amount of circumstellar dust around the stars α Cen A and B. Light scattered and/or thermally emitted by exo-Zodi discs will have profound implications for future spectroscopic missions designed to search for biomarkers in the atmospheres of Earth-like planets. The far-infrared spectral energy distribution of α Cen B is marginally consistent with the presence of a minimum temperature region in the upper atmosphere of the star. We also show that an α Cen A-like temperature minimum may result in an erroneous apprehension about the presence of dust around other, more distant stars. Based on observations with Herschel which is an ESA space observatory with science instruments provided by European-led Principal Investigator consortia and with important participation from NASA.And also based on observations with APEX, which is a 12 m diameter submillimetre telescope at 5100 m altitude on Llano Chajnantor in Chile. The telescope is operated by Onsala Space Observatory, Max-Planck-Institut für Radioastronomie (MPIfR), and European Southern Observatory (ESO).

Lick optical spectra of quasar HS 1946+7658 at 10 kilometers per second resolution Lyman-alpha forest and metal absorption systems

NASA Technical Reports Server (NTRS)

Fan, Xiao-Ming; Tytler, David

1994-01-01

We present optical spectra of the most luminous known quasi stellar object (QSO) HS 1946+7658 (z(sub em) = 3.051). Our spectra have both full wavelength coverage, 3240-10570 A, and in selected regions, either high signal-to-noise ratio, SNR approximately equals 40-100, or unusually high approximately 10 km/sec resolution, and in parts of the Ly alpha forest and to the red of Ly alpha emission they are among the best published. We find 113 Ly alpha systems and six metal-line systems, three of which are new. The metal systems at z(sub abs) = 2.844 and 3.050 have complex velocity structure with four and three prominent components, respectively. We find that the system at z(sub abs) = 2.844 is a damped Ly alpha absorption (DLA) system, with a neutral hydrogen column density of log N(H I) = 20.2 +/- 0.4, and it is the cause of the Lyman limit break at lambda approximately equals 3520 A. We believe that most of the H I column density in this system is in z(sub abs) = 2.8443 component which shows the strongest low-ionization absorption lines. The metal abundance in the gas phase of the system is (M/H) approximately equals -2.6 +/- 0.3, with a best estimate of (M/H) = -2.8, with ionizaion parameter log gamma = -2.75, from a photoionization model. The ratios of the logarithmic abundances of C, O, Al, and Si are all within a factor of 2 of solar, which is important for two reasons. First, we believe that the gas abundances which we measure are close to the total abundances, because the ratio of aluminum to other elements is near cosmic, and Al is a refractory element which depletes very readily like chromium, in the interstellar medium. Second, we do not see the enhancement of O with respect to C of (O/C) approximately equals 0.5-0.9 reported in three partial Lyman limit systems by Reimers et al. (1992) and Vogel & Reimers (1993); we measure (O/C) = -0.06 for observed ions and (O/C) approximately equals 0.2 after ionization corrections, which is consistent with solar abundances. We see C II*(lambda 1335) offset by 15 km/sec with respect to C II(lambda 1334), presumably because the gas density varies from 2 to 8 cm(exp -3) with changing velocity in the DLA system. These densities imply that the damped component is 6-25 pc thick, which is reasonable for a single cloud in a cold spiral disk. They also imply that the cloud is relatively highly ionized with more C III than C II, more O III than O I, and log N(H I) = 20.72, which is 3 times the H I column. The system at z(abs) = 1.7382 is also believed to be damped with N(H I) approximately equals 10(exp 21) cm(exp -2), because we see Cr II, but its Ly alpha line will never be seen because it is below the Lyman limit of the other DLA system. We see a 2.6 sigma lack of Ly alpha forest lines well away from the QSO redshift, which may be a chance fluctuation. We also see a correlation between column density N(H I) and Doppler parameter b for 96 unsaturated Ly Alpha forest absorption lines, and although this correlation persists in the 36 Ly alpha lines which lie in regions where the SNR approximately equals 8-16, we agree with Rauch et al. (1993) that it is probably a bogus effect of low supernova remnant (SNR). The same applies to lines with very low b values: in regions where SNR less than or equal to 8 we see many Ly alpha lines which appear to have 10 less than or equal to b less than or equal to 20, but when 8 less than or equal to SNR less than or equal to 16 we see only one line with b less than or equal to 15 km/sec, and two others which we believe have b less than or equal to 20, with values of 20 and 16 km/sec. Traditional Ly alpha line samples which include all lines which have W/sigma(W) greater than or equal to 4 are not adequate to explore the distribution of the properties of individual clouds, because we need much higher (W/sigma(W)) and SNR to avoid the strong biases.

ESTABLISHING {alpha} Oph AS A PROTOTYPE ROTATOR: IMPROVED ASTROMETRIC ORBIT

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hinkley, Sasha; Hillenbrand, Lynne; Crepp, Justin R.

2011-01-10

The nearby star {alpha} Oph (Ras Alhague) is a rapidly rotating A5IV star spinning at {approx} 89% of its breakup velocity. This system has been imaged extensively by interferometric techniques, giving a precise geometric model of the star's oblateness and the resulting temperature variation on the stellar surface. Fortuitously, {alpha} Oph has a previously known stellar companion, and characterization of the orbit provides an independent, dynamically based check of both the host star and the companion mass. Such measurements are crucial to constrain models of such rapidly rotating stars. In this study, we combine eight years of adaptive optics imagingmore » data from the Palomar, AEOS, and CFHT telescopes to derive an improved, astrometric characterization of the companion orbit. We also use photometry from these observations to derive a model-based estimate of the companion mass. A fit was performed on the photocenter motion of this system to extract a component mass ratio. We find masses of 2.40{sup +0.23}{sub -0.37} M{sub sun} and 0.85{sup +0.06}{sub -0.04} M{sub sun} for {alpha} Oph A and {alpha} Oph B, respectively. Previous orbital studies of this system found a mass too high for this system, inconsistent with stellar evolutionary calculations. Our measurements of the host star mass are more consistent with these evolutionary calculations, but with slightly higher uncertainties. In addition to the dynamically derived masses, we use IJHK photometry to derive a model-based mass for {alpha} Oph B, of 0.77 {+-} 0.05 M{sub sun} marginally consistent with the dynamical masses derived from our orbit. Our model fits predict a periastron passage on 2012 April 19, with the two components having a 50 mas separation from 2012 March to May. A modest amount of interferometric and radial velocity data during this period could provide a mass determination of this star at the few percent level.« less

Vedolizumab is an effective alternative in inflammatory bowel disease patients with anti-TNF-alpha therapy-induced dermatological side effects.

PubMed

Pijls, Philippe A R R; Gilissen, Lennard P L

2016-11-01

The treatment of patients with inflammatory bowel diseases has been revolutionized by the introduction of biological therapy with TNF-alpha blockers. However, TNF-alpha blockers are also associated with a wide variety of dermatological side effects, such as local skin infections, psoriasis and eczema. A new biological therapy, targeting the gut-specific adhesion molecule alpha4beta7 integrin, is the humanized monoclonal IgG1 antibody vedolizumab. Vedolizumab prevents leukocyte migration to the gastrointestinal tract, thereby reducing inflammation. This gut-specific therapy has the potential to reduce systemic side effects, including dermatological ones. We describe 3 inflammatory bowel disease patients who experience anti-TNF-alpha therapy-induced dermatological side effects, consisting of hidradenitis suppurativa, a folliculitis, scalp psoriasis and a dissecting folliculitis. In all patients, anti-TNF-alpha therapy-induced dermatological side effects diminished after switching to vedolizumab. Vedolizumab may be a viable alternative biological therapy in inflammatory bowel disease patients who experience anti-TNF-alpha therapy-induced dermatological side effects. Copyright © 2016 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

Alpha 2-macroglobulin capture allows detection of mast cell chymase in serum and creates a reservoir of angiotensin II-generating activity.

PubMed

Raymond, Wilfred W; Su, Sharon; Makarova, Anastasia; Wilson, Todd M; Carter, Melody C; Metcalfe, Dean D; Caughey, George H

2009-05-01

Human chymase is a highly efficient angiotensin II-generating serine peptidase expressed by mast cells. When secreted from degranulating cells, it can interact with a variety of circulating antipeptidases, but is mostly captured by alpha(2)-macroglobulin, which sequesters peptidases in a cage-like structure that precludes interactions with large protein substrates and inhibitors, like serpins. The present work shows that alpha(2)-macroglobulin-bound chymase remains accessible to small substrates, including angiotensin I, with activity in serum that is stable with prolonged incubation. We used alpha(2)-macroglobulin capture to develop a sensitive, microtiter plate-based assay for serum chymase, assisted by a novel substrate synthesized based on results of combinatorial screening of peptide substrates. The substrate has low background hydrolysis in serum and is chymase-selective, with minimal cleavage by the chymotryptic peptidases cathepsin G and chymotrypsin. The assay detects activity in chymase-spiked serum with a threshold of approximately 1 pM (30 pg/ml), and reveals native chymase activity in serum of most subjects with systemic mastocytosis. alpha(2)-Macroglobulin-bound chymase generates angiotensin II in chymase-spiked serum, and it appears in native serum as chymostatin-inhibited activity, which can exceed activity of captopril-sensitive angiotensin-converting enzyme. These findings suggest that chymase bound to alpha(2)-macroglobulin is active, that the complex is an angiotensin-converting enzyme inhibitor-resistant reservoir of angiotensin II-generating activity, and that alpha(2)-macroglobulin capture may be exploited in assessing systemic release of secreted peptidases.

ASYMMETRIC ABSORPTION PROFILES OF Ly{alpha} AND Ly{beta} IN DAMPED Ly{alpha} SYSTEMS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Hee-Won, E-mail: hwlee@sejong.ac.kr

2013-08-01

Damped Ly{alpha} systems observed in the quasar spectra are characterized by a high neutral hydrogen column density, N{sub HI} > 2 x 10{sup 20} cm{sup -2}. The absorption wing profiles are often fitted using the Voigt function due to the fact that the scattering cross section near the resonant line center is approximately described by the Lorentzian function. Since a hydrogen atom has infinitely many p states that participate in the electric dipole interaction, the cross section starts to deviate from the Lorentzian in an asymmetric way in the line wing regions. We investigate this asymmetry in the absorption linemore » profiles around Ly{alpha} and Ly{beta} as a function of the neutral hydrogen column density N{sub HI}. In terms of {Delta}{lambda} {identical_to} {lambda} - {lambda}{sub {alpha}}, we expand the Kramers-Heisenberg formula around Ly{alpha} to find {sigma}({lambda}) {approx_equal} (0.5f{sub 12}){sup 2}{sigma}{sub T}({Delta}{lambda}/{lambda}{sub {alpha}}){sup -2}[1 + 3.792({Delta}{lambda}/{lambda}{sub {alpha}})], where f{sub 12} and {sigma}{sub T} are the oscillator strength of Ly{alpha} and the Thomson scattering cross section, respectively. In terms of {Delta}{lambda}{sub 2} {identical_to} {lambda} - {lambda}{sub {beta}} in the vicinity of Ly{beta}, the total scattering cross section, given as the sum of cross sections for Rayleigh and Raman scattering, is shown to be {sigma}({lambda}) {approx_equal} {sigma}{sub T}(0.5f{sub 13}){sup 2}(1 + R{sub 0})({Delta}{lambda}{sub 2}/{lambda}{sub {beta}}){sup -2}[1 - 24.68({Delta}{lambda}{sub 2}/{lambda}{sub {beta}})] with f{sub 13} and the factor R{sub 0} = 0.1342 being the oscillator strength for Ly{beta} and the ratio of the Raman cross section to Rayleigh cross section, respectively. A redward asymmetry develops around Ly{alpha}, whereas a blue asymmetry is obtained for Ly{beta}. The absorption center shifts are found to be almost proportional to the neutral hydrogen column density.« less

Oscillatory Alpha-Band Suppression Mechanisms during the Rapid Attentional Shifts Required to Perform an Anti-Saccade Task

PubMed Central

Belyusar, Daniel; Snyder, Adam C.; Frey, Hans-Peter; Harwood, Mark R.; Wallman, Josh; Foxe, John J.

2015-01-01

Neuroimaging has demonstrated anatomical overlap between covert and overt attention systems, although behavioral and electrophysiological studies have suggested that the two systems do not rely on entirely identical circuits or mechanisms. In a parallel line of research, topographically-specific modulations of alpha-band power (~8-14Hz) have been consistently correlated with anticipatory states during tasks requiring covert attention shifts. These tasks, however, typically employ cue-target-interval paradigms where attentional processes are examined across relatively protracted periods of time and not at the rapid timescales implicated during overt attention tasks. The anti-saccade task, where one must first covertly attend for a peripheral target, before executing a rapid overt attention shift (i.e. a saccade) to the opposite side of space, is particularly well-suited for examining the rapid dynamics of overt attentional deployments. Here, we asked whether alpha-band oscillatory mechanisms would also be associated with these very rapid overt shifts, potentially representing a common neural mechanism across overt and covert attention systems. High-density electroencephalography in conjunction with infra-red eye-tracking was recorded while participants engaged in both pro- and anti- saccade task blocks. Alpha power, time-locked to saccade onset, showed three distinct phases of significantly lateralized topographic shifts, all occurring within a period of less than one second, closely reflecting the temporal dynamics of anti-saccade performance. Only two such phases were observed during the pro-saccade task. These data point to substantially more rapid temporal dynamics of alpha-band suppressive mechanisms than previously established, and implicate oscillatory alpha-band activity as a common mechanism across both overt and covert attentional deployments. PMID:23041338

Stability and stability degree of a cracked flexible rotor supported on journal bearings

NASA Technical Reports Server (NTRS)

Meng, Guang; Gasch, Robert

1994-01-01

This paper investigates the stability and the stability degree of a flexible cracked rotor supported on different kinds of journal bearings. It is found that no matter what kind of bearings is used, the unstable zones caused by rotor crack locate always within the speed ratio (2/N) (1 - Delta K(sub xi)/4) is less than Omega is less than 2/N when gravity parameter W(sub R) is greater than 1.0, and locate always within the speed ratio (2 Omega(sub alpha)/N) (1 - Delta K(sub xi)/4) is less than Omega is less than 2 Omega(sub alpha)/N when W(sub R) is less than 0.1, where Delta K(sub xi) is the crack stiffness ratio, N = 1, 2, 3, 4, 5, ..., and Omega(sub alpha) = ((1 + 2 alpha)/2 alpha)(exp 1/2). When 0.1 is less than W(sub R) is less than 1.0, there is a region where no unstable zones caused by rotor crack exist. Outside the crack ridge zones, the rotor crack has almost no influence on system's stability and stability degree; while within the crack ridge zones, the stability and stability degree depend both on the crack and system's parameters. In some cases, the system may still be stable even when the crack is very large. For small gravity parameter (W(sub R) is less than 0.1), the mass ratio alpha has large influence on the position of unstable region, but its influence on the stability degree is small. The influence of fixed Sommerfeld number S(sub 0) on the crack stability degree is small although S(sub 0) has large influence on the stability degree of uncracked rotor.

Immune-endocrine interactions in the mammalian adrenal gland: facts and hypotheses.

PubMed

Nussdorfer, G G; Mazzocchi, G

1998-01-01

Several cytokines, which are the major mediators of the inflammatory responses, are well-known to stimulate the hypothalamopituitary corticotropin-releasing hormone (CRH)/adrenocorticotropic hormone (ACTH) system, thereby evoking secretory responses by the adrenal cortex. Many of these cytokines, including interleukin-1 (IL-1), IL-2, IL-6, tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (INF-gamma) are synthesized in the adrenal gland by both parenchymal cells and resident macrophages, and the release of some of them (e.g., IL-6 and TNF-alpha) is regulated by the main agonists of steroid hormone secretion (e.g., ACTH and angiotensin-II) and bacterial endotoxins. Adrenocortical and adrenomedullary cells are provided with specific receptors for IL-1, IL-2, and IL-6. IL-1 and TNF-alpha directly inhibit aldosterone secretion of zona glomerulosa cells, whereas IL-6 enhances it. IL-2, IL-3, IL-6, and INF-alpha are able to directly stimulate glucocorticoid production by zona fasciculata and zona reticularis cells, whereas IL-1 exerts an analogous effect through an indirect mechanism involving the stimulation of catecholamine release by chromaffin cells and/or the activation of the intramedullary CRH/ACTH system; again, TNF-alpha depresses glucocorticoid synthesis. IL-6 raises androgen secretion by inner adrenocortical layers. IL-1 enhances the proliferation of adrenocortical cells, and findings suggest that cytokines may control the apoptotic deletion of senescent zona reticularis cells. The relevance of the intraadrenal cytokine system in the fine-tuning of the secretion and growth of the adrenal cortex under normal conditions remains to be explored. However, indirect proof is available that local immune-endocrine interactions may play an important role in modulating adrenal responses to inflammatory and immune challenges and stresses.

Persistent short-term memory defects following sleep deprivation in a drosophila model of Parkinson disease.

PubMed

Seugnet, Laurent; Galvin, James E; Suzuki, Yasuko; Gottschalk, Laura; Shaw, Paul J

2009-08-01

Parkinson disease (PD) is the second most common neurodegenerative disorder in the United States. It is associated with motor deficits, sleep disturbances, and cognitive impairment. The pathology associated with PD and the effects of sleep deprivation impinge, in part, upon common molecular pathways suggesting that sleep loss may be particularly deleterious to the degenerating brain. Thus we investigated the long-term consequences of sleep deprivation on shortterm memory using a Drosophila model of Parkinson disease. Transgenic strains of Drosophila melanogaster. Using the GAL4-UAS system, human alpha-synuclein was expressed throughout the nervous system of adult flies. Alpha-synuclein expressing flies (alpha S flies) and the corresponding genetic background controls were sleep deprived for 12 h at age 16 days and allowed to recover undisturbed for at least 3 days. Short-term memory was evaluated using aversive phototaxis suppression. Dopaminergic systems were assessed using mRNA profiling and immunohistochemistry. MEASURMENTS AND RESULTS: When sleep deprived at an intermediate stage of the pathology, alpha S flies showed persistent short-term memory deficits that lasted > or = 3 days. Cognitive deficits were not observed in younger alpha S flies nor in genetic background controls. Long-term impairments were not associated with accelerated loss of dopaminergic neurons. However mRNA expression of the dopamine receptors dDA1 and DAMB were significantly increased in sleep deprived alpha S flies. Blocking D1-like receptors during sleep deprivation prevented persistent shortterm memory deficits. Importantly, feeding flies the polyphenolic compound curcumin blocked long-term learning deficits. These data emphasize the importance of sleep in a degenerating/reorganizing brain and shows that pathological processes induced by sleep deprivation can be dissected at the molecular and cellular level using Drosophila genetics.

Mesenteric Th1 polarization and monocyte TNF-alpha production: first steps to systemic inflammation in rats with cirrhosis.

PubMed

Muñoz, Leticia; Albillos, Agustín; Nieto, Mónica; Reyes, Eduardo; Lledó, Lourdes; Monserrat, Jorge; Sanz, Eva; de la Hera, Antonio; Alvarez-Mon, Melchor

2005-08-01

A systemic inflammatory state with increased circulating tumor necrosis factor alpha (TNF-alpha) has been related to the bacterial infection susceptibility and hemodynamic derangement of patients with cirrhosis. We compared the activation status of immune cell subpopulations defined by 4-color cytometry in mesenteric and peripheral lymph nodes and blood of rats with CCl(4)-cirrhosis to define the immune response initiation site, the T-cell and monocyte contribution to pro-inflammatory cytokine production, as well as the pathogenic role of enteric bacteria in the cirrhosis immune response. Th1 cells and monocytes were expanded in the mesenteric nodes (P < .001) and blood (P < .001) of rats with cirrhosis, and activated to produce interferon gamma (P < .0001) and TNF-alpha (P < .0001), respectively. The greater numbers of recently activated CD134(+) Th cells in mesenteric nodes compared with blood, the correlation between their numbers in mesenteric nodes and blood (r = 0.66, P < .001), and the expansion of activated CD45RC(-) Th cells, which are unable to re-enter lymph nodes, in mesenteric nodes but not in blood or axillary nodes points to mesenteric nodes as the origin site of activated Th cells. Abrogation of bacterial translocation by bowel decontamination reduced the number of activated Th cells and monocytes, and normalized interferon gamma production by Th cells and TNF-alpha production by monocytes in mesenteric nodes and blood, respectively. In conclusion, in cirrhosis, enteric bacteria start off an orchestrated immune response cascade in mesenteric nodes involving Th1 polarization and monocyte activation to TNF-alpha production. Later, the recirculation of these activated effector immune cells into blood promotes systemic inflammation.

Engineering design of the PLX- α coaxial gun

NASA Astrophysics Data System (ADS)

Cruz, Edward; Brockington, Samuel; Case, Andrew; Luna, Marco; Witherspoon, Douglas; Langendorf, Samuel

2016-10-01

We describe the engineering and technical aspects of the coaxial gun designed for the 60-gun scaling study of spherically imploding plasma liners as a standoff driver for plasma-jet-driven magneto-inertial fusion. Each coaxial gun incorporates a fast, dense gas injection and triggering system, a compact low-weight pfn with integral sparkgap switching, and a contoured gap designed to suppress the blow-by instability. Alpha1 and Alpha2 guns are compared, with emphasis on the improvements on Alpha2, which include a faster more robust gas valve, an improved electrode contour, a custom 600- μF, 5-kV pfn, and a set of six inline sparkgap switches operated in parallel. The switch and pfn configurations are mounted directly to the back of the gun, and are designed to reduce inductance, cost, and complexity, maximize efficiency and system reliability, and ensure symmetric current flow. We will provide a detailed overview of the design choices made for the PLX- α coaxial gun. This work supported by the ARPA-E ALPHA Program.

Phase transformations involving the [alpha][sub 2] and O phases in Ti-Al-Nb alloys. [Ti-28. 5Al-13Nb

DOE Office of Scientific and Technical Information (OSTI.GOV)

Muraleedharan, K.; Banerjee, D.

1993-08-15

An orthorhombic (O) phase with Cmcm space group and Ti[sub 2]AlNb composition has ben established in the Ti-Al-Nb system. Efforts to develop alloys with this orthorhombic phase as a major phase, in place of the [alpha][sub 2] (Ti[sub 3]Al) phase, resulted in compositions with superior combinations of strength and toughness. The determination of phase diagrams for the Ti-Al-Nb system is a continuing effort. Bendersky et al. considered possible transformation paths and the hierarchy of structures in going from the [beta] phase to [alpha][sub 2] or O phases through displacive or replacive reactions. Microstructures predicted by these considerations have been documentedmore » in the particularly well investigated [beta]-->O transformation. Very little work has however been carried out on the [alpha][sub 2]-->O phase transformation. In this paper, the authors report preliminary results of isothermal aging study of this transformation.« less

Development and irradiation test of lost alpha detection system for ITER.

PubMed

Nishiura, M; Nagasaka, T; Fujioka, K; Fujimoto, Y; Tanaka, T; Ido, T; Yamamoto, S; Kashiwa, S; Sasao, M

2010-10-01

We developed a lost alpha detection system to use in burning plasma experiments. The scintillators of Ag:ZnS and polycrystalline Ce:YAG were designed for a high-temperature environment, and the optical transmission line was designed to transmit from the scintillator to the port plug. The required optical components of lenses and mirrors were irradiated using the fission reactor with the initial result that there was no clear change after the irradiation with a neutron flux of 9.6×10(17) nm(-2)  s(-1) for 48 h. We propose a diagnostic of alpha particle loss, so-called alpha particle induced gamma ray spectroscopy. The initial laboratory test has been carried out by the use of the Ce doped Lu(2)SiO(5) scintillator detector and an Am-Be source to detect the 4.44 MeV high energy gamma ray due to the (9)Be(α,nγ)(12)C reaction.

An atlas of H-alpha-emitting regions in M33: A systematic search for SS433 star candidates

NASA Technical Reports Server (NTRS)

Calzetti, Daniela; Kinney, Anne L.; Ford, Holland; Doggett, Jesse; Long, Knox S.

1995-01-01

We report finding charts and accurate positions for 432 compact H-alpha emitting regions in the Local Group galaxy M 33 (NGC 598), in an effort to isolate candidates for an SS433-like stellar system. The objects were extracted from narrow band images, centered in the rest-frame H-alpha (lambda 6563 A) and in the red continuum at 6100 A. The atlas is complete down to V approximately equal to 20 and includes 279 compact HII regions and 153 line emitting point-like sources. The point-like sources undoubtedly include a variety of objects: very small HII regions, early type stars with intense stellar winds, and Wolf-Rayet stars, but should also contain objects with the characteristics of SS433. This extensive survey of compact H-alpha regions in M 33 is a first step towards the identification of peculiar stellar systems like SS433 in external galaxies.

Frontal alpha asymmetry and sexually motivated states.

PubMed

Prause, Nicole; Staley, Cameron; Roberts, Verena

2014-03-01

Anterior alpha asymmetry of electroencephalographic (EEG) signals has been suggested to index state approach (or avoidance) motivation. This model has not yet been extended to high approach-motivation sexual stimuli, which may represent an important model of reward system function. Sixty-five participants viewed a neutral and a sexually motivating film while their EEG was recorded, and reported their sexual feelings after each film. Greater alpha power in the left hemisphere during sexually motivated states was evident. A positive relationship between self-reported mental sexual arousal and alpha asymmetry was identified, where coherence between these indicators was higher in women. Notably, coherence was stronger when mental versus physical sexual arousal was rated. Alpha asymmetry appears to offer a new method for further examining this novel coherence pattern across men and women. Copyright © 2014 Society for Psychophysiological Research.

Defects in adaptive energy metabolism with CNS-linked hyperactivity in PGC-1alpha null mice.

PubMed

Lin, Jiandie; Wu, Pei-Hsuan; Tarr, Paul T; Lindenberg, Katrin S; St-Pierre, Julie; Zhang, Chen-Yu; Mootha, Vamsi K; Jäger, Sibylle; Vianna, Claudia R; Reznick, Richard M; Cui, Libin; Manieri, Monia; Donovan, Mi X; Wu, Zhidan; Cooper, Marcus P; Fan, Melina C; Rohas, Lindsay M; Zavacki, Ann Marie; Cinti, Saverio; Shulman, Gerald I; Lowell, Bradford B; Krainc, Dimitri; Spiegelman, Bruce M

2004-10-01

PGC-1alpha is a coactivator of nuclear receptors and other transcription factors that regulates several metabolic processes, including mitochondrial biogenesis and respiration, hepatic gluconeogenesis, and muscle fiber-type switching. We show here that, while hepatocytes lacking PGC-1alpha are defective in the program of hormone-stimulated gluconeogenesis, the mice have constitutively activated gluconeogenic gene expression that is completely insensitive to normal feeding controls. C/EBPbeta is elevated in the livers of these mice and activates the gluconeogenic genes in a PGC-1alpha-independent manner. Despite having reduced mitochondrial function, PGC-1alpha null mice are paradoxically lean and resistant to diet-induced obesity. This is largely due to a profound hyperactivity displayed by the null animals and is associated with lesions in the striatal region of the brain that controls movement. These data illustrate a central role for PGC-1alpha in the control of energy metabolism but also reveal novel systemic compensatory mechanisms and pathogenic effects of impaired energy homeostasis.

Utilization of peptide carrier system to improve intestinal absorption: targeting prolidase as a prodrug-converting enzyme

NASA Technical Reports Server (NTRS)

Bai, J. P.; Hu, M.; Subramanian, P.; Mosberg, H. I.; Amidon, G. L.

1992-01-01

The feasibility of targeting prolidase as a peptide prodrug-converting enzyme has been examined. The enzymatic hydrolysis by prolidase of substrates for the peptide transporter L-alpha-methyldopa-pro and several dipeptide analogues without an N-terminal alpha-amino group (phenylpropionylproline, phenylacetylproline, N-benzoylproline, and N-acetylproline) was investigated. The Michaelis-Menten parameters Km and Vmax for L-alpha-methyldopa-pro are 0.09 +/- 0.02 mM and 3.98 +/- 0.25 mumol/min/mg protein, respectively. However, no hydrolysis of the dipeptide analogues without an N-terminal alpha-amino group is observed, suggesting that an N-terminal alpha-amino group is required for prolidase activity. These results demonstrate that prolidase may serve as a prodrug-converting enzyme for the dipeptide-type prodrugs, utilizing the peptide carrier for transport of prodrugs into the mucosal cells and prolidase, a cytosolic enzyme, to release the drug. However, a free alpha-amino group appears to be necessary for prolidase hydrolysis.

The octamer-binding proteins form multi-protein--DNA complexes with the HSV alpha TIF regulatory protein.

PubMed Central

Kristie, T M; LeBowitz, J H; Sharp, P A

1989-01-01

The herpes simplex virus transactivator, alpha TIF, stimulates transcription of the alpha/immediate early genes via a cis-acting site containing an octamer element and a conserved flanking sequence. The alpha TIF protein, produced in a baculovirus expression system, nucleates the formation of at least two DNA--protein complexes on this regulatory element. Both of these complexes contain the ubiquitous Oct-1 protein, whose POU domain alone is sufficient to allow assembly of the alpha TIF-dependent complexes. A second member of the POU domain family, the lymphoid specific Oct-2 protein, can also be assembled into similar complexes at high concentrations of alpha TIF protein. These complexes contain at least two cellular proteins in addition to Oct-1. One of these proteins is present in both insect and HeLa cells and probably recognizes sequences in the cis element. The second cellular protein, only present in HeLa cells, probably binds by protein-protein interactions. Images PMID:2556266

The octamer-binding proteins form multi-protein--DNA complexes with the HSV alpha TIF regulatory protein.

PubMed

Kristie, T M; LeBowitz, J H; Sharp, P A

1989-12-20

The herpes simplex virus transactivator, alpha TIF, stimulates transcription of the alpha/immediate early genes via a cis-acting site containing an octamer element and a conserved flanking sequence. The alpha TIF protein, produced in a baculovirus expression system, nucleates the formation of at least two DNA--protein complexes on this regulatory element. Both of these complexes contain the ubiquitous Oct-1 protein, whose POU domain alone is sufficient to allow assembly of the alpha TIF-dependent complexes. A second member of the POU domain family, the lymphoid specific Oct-2 protein, can also be assembled into similar complexes at high concentrations of alpha TIF protein. These complexes contain at least two cellular proteins in addition to Oct-1. One of these proteins is present in both insect and HeLa cells and probably recognizes sequences in the cis element. The second cellular protein, only present in HeLa cells, probably binds by protein-protein interactions.

Neuronal nicotinic acetylcholine receptor subunits in autism: an immunohistochemical investigation in the thalamus.

PubMed

Ray, M A; Graham, A J; Lee, M; Perry, R H; Court, J A; Perry, E K

2005-08-01

The cholinergic system has been implicated in the development of autism on the basis of neuronal nicotinic acetylcholine receptor (nAChR) losses in cerebral and cerebellar cortex. In the present study, the first to explore nAChRs in the thalamus in autism, alpha4, alpha7 and beta2 nAChR subunit expression in thalamic nuclei of adult individuals with autism (n=3) and age-matched control cases (n=3) was investigated using immunochemical methods. Loss of alpha7- and beta2- (but not alpha4-) immunoreactive neurons occurred in the paraventricular nucleus (PV) and nucleus reuniens in autism. Preliminary results indicated glutamic acid decarboxylase immunoreactivity occurred at a low level in PV, co-expressed with alpha7 in normal and autistic cases and was not reduced in autism. This suggested loss of neuronal alpha7 in autism is not caused by loss of GABAergic neurons. These findings indicate nicotinic abnormalities that occur in the thalamus in autism which may contribute to sensory or attentional deficits.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Testa, U.; Hinard, N.; Beuzard, Y.

During incubation of reticulocytes from patients with beta-thalassemia, after labeling of the hemoglobin with radioactive amino acids, the excess alpha chains are gradually lost from the cells. The aim of this study was to investigate the mechanism of this phenomenon. A system was developed in which reticulocytes from beta-thalassemia patients are labeled with (3H)leucine, washed several times in nonradioactive medium, and then incubated in the same medium containing puromycin added in order to stop further protein synthesis. The results have clearly shown that excess alpha chains are gradually degraded by proteolysis. N-ethylmaleimide or epsilon-aminocaproic acid inhibited the proteolysis of freemore » alpha chains. The addition of either ATP or hemin did not change the rate of alpha chain degradation. The time required to degrade 50% of the pool of free alpha chains was directly dependent on the initial value of this pool. This finding suggests the absence of a significant individual variation in the ability to proteolyse free alpha chains.« less

A Comparison of Two Chord Keyboard Coding Systems for Alphanumeric Data Entry.

DTIC Science & Technology

1987-06-01

These rates were achieved while typing new material with no more than two errors per minute. No IBM machine has yet been marketed using this chord...type", subject’s typing rates on the standard typewriter ranged from 54.5 CPM to 176.5 CPM for mixed alphanumeric data. Nine of the ten subjects...dot Keyboard and the Standard Keyboard Material Keyboard Entry Rate All Letters Alpha-dot Keyboard 65.25 CPM Mixed Alpha/Numeric Text Alpha-dot

Measurement system for alpha emitters in solution

NASA Astrophysics Data System (ADS)

Robert, A.; Sella, C.; Heindl, R.

1984-08-01

The measurement of alpha emitter concentrations in solution corresponds to a need felt in particular by laboratories working on actinides and in the spent fuel reprocessing industry. The instrument present here allows this measurement continuously by the use of a new scintillator that is insensitive to corrosive liquids. The extreme thinness of the scintillator guarantees good detection selectivity of alpha particles in the presence of beta and gamma emissions. Examples of uranium-233, plutonium-239 and americium-241 concentration measurements are presented.

A biochemical logic gate using an enzyme and its inhibitor. Part II: The logic gate.

PubMed

Sivan, Sarit; Tuchman, Samuel; Lotan, Noah

2003-06-01

Enzyme-Based Logic Gates (ENLOGs) are key components in bio-molecular systems for information processing. This report and the previous one in this series address the characterization of two bio-molecular switching elements, namely the alpha-chymotrypsin (alphaCT) derivative p-phenylazobenzoyl-alpha-chymotrypsin (PABalphaCT) and its inhibitor (proflavine), as well as their assembly into a logic gate. The experimental output of the proposed system is expressed in terms of enzymic activity and this was translated into logic output (i.e. "1" or "0") relative to a predetermined threshold value. We have found that an univalent link exists between the dominant isomers of PABalphaCT (cis or trans), the dominant form of either acridine (proflavine) or acridan and the logic output of the system. Thus, of all possible combinations, only the trans-PABalphaCT and the acridan lead to an enzymic activity that can be defined as logic output "1". The system operates under the rules of Boolean algebra and performs as an "AND" logic gate.

Characterization of antibodies that selectively detect alpha-synuclein in pathological inclusions.

PubMed

Waxman, Elisa A; Duda, John E; Giasson, Benoit I

2008-07-01

Sensitive detection of alpha-synuclein (alpha-syn) pathology is important in the diagnosis of disorders like Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy and in providing better insights into the etiology of these diseases. Several monoclonal antibodies that selectively react with aggregated alpha-syn in pathological inclusions and reveal extensive and underappreciated alpha-syn pathology in the brains of diseased patients were previously reported by Duda et al. (Ann Neurol 52:205-210, 2002). We sought to characterize the specificity of some of these antibodies (Syn 505, Syn 506 and Syn 514); using C-terminal and N-terminal truncations of alpha-syn, all three antibodies were determined to require N-terminal epitopes that minimally comprise amino acids 2-4, but possibly extend to amino acid 12 of alpha-syn. The selectivity of these antibodies was further assessed using biochemical analysis of human brains and reactivity to altered recombinant alpha-syn proteins with duplication variants of amino acids 1-12. In addition, by expressing wild-type or a double mutant (E46K/A53T) of alpha-syn in cultured cells and by comparing their immunoreactivities to another antibody (SNL-4), which has a similar primary epitope, it was determined that Syn 505, Syn 506 and Syn 514 recognize conformational variants of alpha-syn that is enhanced by the presence of the double mutations. These studies indicate that antibodies Syn 505, Syn 506 and Syn 514 preferentially recognize N-terminal epitopes in complex conformations, consistent with the dramatic conformational change associated with the polymerization of alpha-synuclein into amyloid fibrils that form pathological inclusions.

Tryptophan W207 in transducin T alpha is the fluorescence sensor of the G protein activation switch and is involved in the effector binding.

PubMed Central

Faurobert, E; Otto-Bruc, A; Chardin, P; Chabre, M

1993-01-01

We have produced a recombinant transducin alpha subunit (rT alpha) in sf9 cells, using a baculovirus system. Deletion of the myristoylation site near the N-terminal increased the solubility and allowed the purification of rT alpha. When reconstituted with excess T beta gamma on retinal membrane, rT alpha displayed functional characteristics of wild-type T alpha vis à vis its coupled receptor, rhodopsin and its effector, cGMP phosphodiesterase (PDE). We further mutated a tryptophan, W207, which is conserved in all G proteins and is suspected to elicit the fluorescence change correlated to their activation upon GDP/GTP exchange or aluminofluoride (AlFx) binding. [W207F]T alpha mutant displayed high affinity receptor binding and underwent a conformational switch upon receptor-catalysed GTP gamma S binding or upon AlFx binding, but this did not elicit any fluorescence change. Thus W207 is the only fluorescence sensor of the switch. Upon the switch the mutant remained unable to activate the PDE. To characterize better its effector-activating interaction we measured the affinity of [W207F]T alpha GDP-AlFx for PDE gamma, the effector subunit that binds most tightly to T alpha. [W207F]T alpha still bound in an activation-dependent way to PDE gamma, but with a 100-fold lower affinity than rT alpha. This suggests that W207 contributes to the G protein effector binding. Images PMID:8223434

The role of TNF alpha polymorphism and expression in susceptibility to nasal polyposis.

PubMed

Zhang, Guimin; Zhang, Jinmei; Kuang, Manbao; Lin, Peng

2018-05-01

In this study, we first performed a meta-analysis to assess the role of single-nucleotide polymorphism (SNP) within tumor necrosis factor alpha (TNF alpha) gene and TNF alpha expression in the risk of nasal polyposis. STATA 12.0 software was utilized to conduct the Mantel-Haenszel statistics, Cohen statistics, Begg's test, Egger's tests and sensitivity analysis. We systemically carried out the database retrieval and initially identified 486 articles. After screening, 15 articles were included in our meta-analysis. For TNF alpha rs1800629 G/A SNP, compared with control group, an increased risk of nasal polyposis of case group was observed in the models of A vs. G [p (P value of association) = 0.009, OR (odds ratio) = 1.35], GA vs. GG (p = 0.001, OR = 1.69), GA+AA vs. GG (p = 0.010, OR = 1.47). The similar results were observed in Caucasian subgroup (p < 0.05, OR > 1). For TNF alpha rs361525 G/A SNP, no significant difference between control and case group was detected (all p > 0.05). In addition, a significant difference exists between case and control groups in the meta-analyses of TNF alpha expression in nasal mucosal cells, secreted TNF alpha (p < 0.05, OR > 1), but not serum TNF alpha (p = 0.090). The present meta-analysis revealed that TNF alpha rs1800629, increased TNF alpha expression and secretion of nasal mucosal cells were associated with an increased risk of nasal polyposis.

The MagAO Giant Accreting Protoplanet Survey (GAPlanetS): Recent Results

NASA Astrophysics Data System (ADS)

Follette, Katherine; Close, Laird; Males, Jared; Morzinski, Katie; Leonard, Clare; MagAO

2018-01-01

I will summarize recent results of the MagAO Giant Accreting Protoplant Survey (GAPlanetS), a search for accreting protoplanets at H-alpha inside of transitional disk gaps. These young, centrally-cleared circumstellar disks are often hosted by stars that are still actively accreting, making it likely that any planets that lie in their central cavities will also be actively accreting. Through differential imaging at Hydrogen-alpha using Magellan's visible light adaptive optics system, we have completed the first systematic search for H-alpha emission from accreting protoplanets in fifteen bright Southern hemisphere transitional disks. I will present results from this survey, including a second epoch on the LkCa 15 system that shows several accreting protoplanet candidates.

Topics in the two-dimensional sampling and reconstruction of images. [in remote sensing

NASA Technical Reports Server (NTRS)

Schowengerdt, R.; Gray, S.; Park, S. K.

1984-01-01

Mathematical analysis of image sampling and interpolative reconstruction is summarized and extended to two dimensions for application to data acquired from satellite sensors such as the Thematic mapper and SPOT. It is shown that sample-scene phase influences the reconstruction of sampled images, adds a considerable blur to the average system point spread function, and decreases the average system modulation transfer function. It is also determined that the parametric bicubic interpolator with alpha = -0.5 is more radiometrically accurate than the conventional bicubic interpolator with alpha = -1, and this at no additional cost. Finally, the parametric bicubic interpolator is found to be suitable for adaptive implementation by relating the alpha parameter to the local frequency content of an image.

[Engineering of a System for the Production of Mutant Human Alpha-Fetoprotein in the Methylotrophic Yeast Pichia pastoris].

PubMed

Morozkina, E V; Vavilova, E A; Zatsepin, S S; Klyachko, E V; Yagudin, T A; Chulkin, A M; Dudich, I V; Semenkova, L N; Churilova, I V; Benevolenskii, S V

2016-01-01

A system for the production of mutant recombinant human alpha-fetoprotein (rhAFPO) lacking the glycosylation site has been engineered in the yeast Pichia pastoris. A strain of the methylotrophic yeast Pichia pastoris GS 115/pPICZ?A/rhAFP0, which produces unglycosylated rhAFPO and secretes it to the culture medium, has been constructed. Optimization and scale-up of the fermentation technology have resulted in an increase in the rhAFP0 yield to 20 mg/L. A scheme of isolation and purification of biologically active rhAFP0 has been developed. The synthesized protein has the antitumor activity, which is analogous to the activity of natural human embryonic alpha-fetoprotein.

Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis.

PubMed

Acar, Leyla; Atalan, Nazan; Karagedik, E Hande; Ergen, Arzu

2018-01-20

The humoral system is activated and various cytokines are released due to infections in tissues and traumatic damage. Nuclear factor-kappa B dimers are encoded by nuclear factor-kappa B genes and regulate transcription of several crucial proteins of inflammation such as tumour necrosis factor-alpha. To investigate the possible effect of polymorphisms on tumour necrosis factor-alpha serum levels with clinical and prognostic parameters of sepsis by determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A) gene polymorphisms and tumour necrosis factor-alpha serum levels. Case-control study. Seventy-two patients with sepsis and 104 healthy controls were included in the study. In order to determine the polymorphisms of nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A), polymerase chain reaction-restriction fragment length polymorphism analysis was performed and serum tumour necrosis factor-alpha levels were determined using an enzyme-linked immunosorbent assay. We observed no significant differences in tumour necrosis factor-alpha serum levels between the study groups. In the patient group, an increase in the tumour necrosis factor-alpha serum levels in patients carrying the tumour necrosis factor-alpha (-308 G/A) A allele compared to those without the A allele was found to be statistically significant. Additionally, an increase in the tumour necrosis factor-alpha serum levels in patients carrying tumour necrosis factor-alpha (-308 G/A) AA genotype compared with patients carrying the AG or GG genotypes was statistically significant. No significant differences were found in these 2 polymorphisms between the patient and control groups (p>0.05). Our results showed the AA genotype and the A allele of the tumour necrosis factor-alpha (-308 G/A) polymorphism may be used as a predictor of elevated tumour necrosis factor-alpha levels in patients with sepsis.

The deployment of intersensory selective attention: a high-density electrical mapping study of the effects of theanine.

PubMed

Gomez-Ramirez, Manuel; Higgins, Beth A; Rycroft, Jane A; Owen, Gail N; Mahoney, Jeannette; Shpaner, Marina; Foxe, John J

2007-01-01

: Ingestion of the nonproteinic amino acid theanine (5-N-ethylglutamine) has been shown to increase oscillatory brain activity in the so-called alpha band (8-14 Hz) during resting electroencephalographic recordings in humans. Independently, alpha band activity has been shown to be a key component in selective attentional processes. Here, we set out to assess whether theanine would cause modulation of anticipatory alpha activity during selective attentional deployments to stimuli in different sensory modalities, a paradigm in which robust alpha attention effects have previously been established. : Electrophysiological data from 168 scalp electrode channels were recorded while participants performed a standard intersensory attentional cuing task. : As in previous studies, significantly greater alpha band activity was measured over parieto-occipital scalp for attentional deployments to the auditory modality than to the visual modality. Theanine ingestion resulted in a substantial overall decrease in background alpha levels relative to placebo while subjects were actively performing this demanding attention task. Despite this decrease in background alpha activity, attention-related alpha effects were significantly greater for the theanine condition. : This increase of attention-related anticipatory alpha over the right parieto-occipital scalp suggests that theanine may have a specific effect on the brain's attention circuitry. We conclude that theanine has clear psychoactive properties, and that it represents a potentially interesting, naturally occurring compound for further study, as it relates to the brain's attentional system.

[Effect of TNF-alpha gene polymorphism on outcome of thalidomide-based regimens for multiple myeloma].

PubMed

DU, Juan; Yuan, Zhen-Gang; Zhang, Chun-Yang; Fu, Wei-Jun; Jiang, Hua; Chen, Bao-An; Hou, Jian

2009-10-01

To evaluate the effect of polymorphism at the -238 and -308 position of the TNF-alpha promotor region on the clinical outcome of thalidomide (Thal)-based regimens for the treatment of multiple myeloma (MM). The polymorphism at the -238 and -308 position of the TNF-alpha promotor region of 168 MM patients treated with Thal-based regimens were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genotypes were tested for association with overall response by logistic regression, and survival was evaluated by univariate and multivariate analysis. In TNF-alpha -238 position, 11 (6.5%) patients had GA genotype and 1 (0.6%) AA genotype. In TNF-alpha -308 position, 19 (11.3%) had GA genotype and 1 (0.6%) AA genotype. In univariate analysis, the TNF-alpha -238 GA + AA genotypes were associated with a significantly prolonged progression free survival (PFS) (P = 0.017), and a better overall survival (OS) (P = 0.150). Multivariate COX regression analysis showed that TNF-alpha -238 polymorphic status was an independent prognostic factor for prolonged PFS (P = 0.049). The TNF-alpha -238 polymorphic status is associated with a favorable clinical outcome in MM patients treated with thalidomide-based regimen. The polymorphism status of TNF-alpha gene might be of promise for developing a more informative stratification system for MM.

Comparative inhibition of rabbit globin mRNA translation by modified antisense oligodeoxynucleotides.

PubMed Central

Cazenave, C; Stein, C A; Loreau, N; Thuong, N T; Neckers, L M; Subasinghe, C; Hélène, C; Cohen, J S; Toulmé, J J

1989-01-01

We have studied the translation of rabbit globin mRNA in cell free systems (reticulocyte lysate and wheat germ extract) and in microinjected Xenopus oocytes in the presence of anti-sense oligodeoxynucleotides. Results obtained with the unmodified all-oxygen compounds were compared with those obtained when phosphorothioate or alpha-DNA was used. In the wheat germ system a 17-mer sequence targeted to the coding region of beta-globin mRNA was specifically inhibitory when either the unmodified phosphodiester oligonucleotide or its phosphorothioate analogue were used. In contrast no effect was observed with the alpha-oligomer. These results were ascribed to the fact that phosphorothioate oligomers elicit an RNase-H activity comparable to the all-oxygen congeners, while alpha-DNA/mRNA hybrids were a poor substrate. Microinjected Xenopus oocytes followed a similar pattern. The phosphorothioate oligomer was more efficient to prevent translation than the unmodified 17-mer. Inhibition of beta-globin synthesis was observed in the nanomolar concentration range. This result can be ascribed to the nuclease resistance of phosphorothioates as compared to natural phosphodiester linkages, alpha-oligomers were devoid of any inhibitory effect up to 30 microM. Phosphorothioate oligodeoxyribonucleotides were shown to be non-specific inhibitors of protein translation, at concentrations in the micromolar range, in both cell-free systems and oocytes. Non-specific inhibition of translation was dependent on the length of the phosphorothioate oligomer. These non-specific effects were not observed with the unmodified or the alpha-oligonucleotides. Images PMID:2472605

Development of a high-speed H-alpha camera system for the observation of rapid fluctuations in solar flares

NASA Technical Reports Server (NTRS)

Kiplinger, Alan L.; Dennis, Brian R.; Orwig, Larry E.; Chen, P. C.

1988-01-01

A solid-state digital camera was developed for obtaining H alpha images of solar flares with 0.1 s time resolution. Beginning in the summer of 1988, this system will be operated in conjunction with SMM's hard X-ray burst spectrometer (HXRBS). Important electron time-of-flight effects that are crucial for determining the flare energy release processes should be detectable with these combined H alpha and hard X-ray observations. Charge-injection device (CID) cameras provide 128 x 128 pixel images simultaneously in the H alpha blue wing, line center, and red wing, or other wavelength of interest. The data recording system employs a microprocessor-controlled, electronic interface between each camera and a digital processor board that encodes the data into a serial bitstream for continuous recording by a standard video cassette recorder. Only a small fraction of the data will be permanently archived through utilization of a direct memory access interface onto a VAX-750 computer. In addition to correlations with hard X-ray data, observations from the high speed H alpha camera will also be correlated and optical and microwave data and data from future MAX 1991 campaigns. Whether the recorded optical flashes are simultaneous with X-ray peaks to within 0.1 s, are delayed by tenths of seconds or are even undetectable, the results will have implications on the validity of both thermal and nonthermal models of hard X-ray production.

The stars with H-alpha missing

NASA Technical Reports Server (NTRS)

Downes, Ronald A.

1987-01-01

During the course of a survey for ultraviolet-excess objects, a group of objects were found that spectroscopically resemble the subdwarf B stars, except for variable strength H-alpha absorption. The H-alpha line can have the nominal strength predicted from the other Balmer lines, or it can be completely absent. Observations reveal significant changes in the H-alpha profile on both a night-to-night and month-to-month basis. IUE observations of three stars reveal few features in the ultraviolet region and, when combined with optical data, allow a rough determination of photospheric temperatures. A binary model is proposed for these systems, and the ramifications of the objects found here and in the Palomar-Green survey are discussed.

Ultraviolet observations of alpha Aurigae from Copernicus

NASA Technical Reports Server (NTRS)

Dupree, A. K.

1975-01-01

Emission lines of L-alpha (1215.67 A) and O VI (1031.94 A) were detected in the spectroscopic binary alpha Aur (Capella) with the Princeton experiment on Copernicus. Temperatures of the emitting regions are inferred to be in excess of 300,000 K. The temperature and emission measure are consistent with a variable source of soft X-rays. If the emission is attributed to the primary star (G5 III), the atmosphere is expanding with velocities of about 20-100 km/s. Such expansion can lead to material within the binary system. The density of interstellar hydrogen inferred from absorption of stellar L-alpha appears to be approximately 0.01 hydrogen atoms per cu cm.

Alpha-ray detection with a MgB 2 transition edge sensor

NASA Astrophysics Data System (ADS)

Okayasu, S.; Katagiri, M.; Hojou, K.; Morii, Y.; Miki, S.; Shimakage, H.; Wang, Z.; Ishida, T.

2008-09-01

We have been investigating for neutron detection with the MgB 2 transition edge sensor (TES). For the purpose, we have been developing a low noise measurement system for the detection. To confirm the performance of the detecting sensor, alpha ray detection from an americium-241 ( 241Am) alpha-ray source was achieved. A short microfabricated sample with 10 μm length and 1 μm width is used to improve the S/N ratio. The detection is achieved under a constant current condition in the range between 1 and 6 μA bias current, and the resistivity changes at the sample due to the alpha ray irradiation is detected just on the transition edge.

[Low level alpha activity measurements with pulse shape discrimination--the analytical system and its characteristics].

PubMed

Noguchi, M; Satoh, K; Higuchi, H

1984-12-01

Pulse shape discrimination of alpha and beta rays with liquid scintillation counting was investigated for the purpose of low level alpha activity measurements. Various liquid scintillators for pulse shape discrimination were examined by means of pulse rise time analysis. A new scintillator of low cost and of superior characteristics was found. The figure of merits better than 3.5 in rise time spectrum and the energy resolution better than 9% were obtained for carefully prepared samples. The background counting rate for a sample of 10 ml was reduced to 0.013 cpm/MeV in the range of alpha ray energy 5 to 7 MeV.

Lewis base activation of Lewis acids: catalytic, enantioselective addition of glycolate-derived silyl ketene acetals to aldehydes.

PubMed

Denmark, Scott E; Chung, Won-Jin

2008-06-20

A catalytic system involving silicon tetrachloride and a chiral, Lewis basic bisphosphoramide catalyst is effective for the addition of glycolate-derived silyl ketene acetals to aldehydes. It was found that the sense of diastereoselectivity could be modulated by changing the size of the substituents on the silyl ketene acetals. In general, the trimethylsilyl ketene acetals derived from methyl glycolates with a large protecting group on the alpha-oxygen provide enantiomerically enriched alpha,beta-dihydroxy esters with high syn-diastereoselectivity, whereas the tert-butyldimethylsilyl ketene acetals derived from bulky esters of alpha-methoxyacetic acid provide enantiomerically enriched alpha,beta-dihydroxy esters with high anti-diastereoselecitvity.

Cytokine dysregulation in AIDS: in vivo overexpression of mRNA of tumor necrosis factor-alpha and its correlation with that of the inflammatory cytokine GRO.

PubMed

Dezube, B J; Pardee, A B; Beckett, L A; Ahlers, C M; Ecto, L; Allen-Ryan, J; Anisowicz, A; Sager, R; Crumpacker, C S

1992-01-01

The human immunodeficiency virus establishes an intimate interaction with the immune system. The virus can use cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (Il-1), to regulate its own expression by modifying the normal immunoregulatory network. We demonstrate that mRNA of the cytokine TNF-alpha from peripheral blood mononuclear cells is overexpressed in virtually all patients with AIDS who do not have active opportunistic infections compared with uninfected volunteers (p < 0.0001). This overexpression correlates with elevated mRNA levels of the recently discovered GRO (p < 0.05), a cytokine involved in the inflammatory response.

Nefiracetam facilitates hippocampal neurotransmission by a mechanism independent of the piracetam and aniracetam action.

PubMed

Nomura, T; Nishizaki, T

2000-07-07

Nefiracetam, a nootropic (cognition-enhancing) agent, facilitated neurotransmission in the dentate gyrus of rat hippocampal slices in a dose-dependent manner at concentrations ranged from 1 nM to 1 microM, being evident at 60-min washing-out of the drug. The facilitatory action was blocked by the nicotinic acetylcholine (ACh) receptor antagonists, alpha-bungarotoxin and mecamylamine. A similar facilitation was induced by the other nootropic agents, piracetam and aniracetam, but the facilitation was not inhibited by nicotinic ACh receptor antagonists and it did not occlude the potentiation induced by nefiracetam. In the Xenopus oocyte expression systems, nefiracetam potentiated currents through a variety of neuronal nicotinic ACh receptors (alpha 3beta 2, alpha 3beta 4, alpha 4 beta 2, alpha 4 beta 4, and alpha 7) to a different extent. In contrast, neither piracetam nor aniracetam had any potentiating action on alpha 7 receptor currents. While aniracetam delayed the decay time of currents through the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor, GluR1, -2, -3, expressed in oocytes, nefiracetam or piracetam had no effect on the currents. Nefiracetam, thus, appears to facilitate hippocampal neurotransmission by functionally targeting nicotinic ACh receptors, independently of the action of piracetam and aniracetam.

Ex vivo study on root canal instrumentation of two rotary nickel-titanium systems in comparison to stainless steel hand instruments.

PubMed

Vaudt, J; Bitter, K; Neumann, K; Kielbassa, A M

2009-01-01

To investigate instrumentation time, working safety and the shaping ability of two rotary nickel-titanium (NiTi) systems (Alpha System and ProTaper Universal) in comparison to stainless steel hand instruments. A total of 45 mesial root canals of extracted human mandibular molars were selected. On the basis of the degree of curvature the matched teeth were allocated randomly into three groups of 15 teeth each. In group 1 root canals were prepared to size 30 using a standardized manual preparation technique; in group 2 and 3 rotary NiTi instruments were used following the manufacturers' instructions. Instrumentation time and procedural errors were recorded. With the aid of pre- and postoperative radiographs, apical straightening of the canal curvature was determined. Photographs of the coronal, middle and apical cross-sections of the pre- and postoperative canals were taken, and superimposed using a standard software. Based on these composite images the portion of uninstrumented canal walls was evaluated. Active instrumentation time of the Alpha System was significantly reduced compared with ProTaper Universal and hand instrumentation (P < 0.05; anova). No instrument fractures occurred in any of the groups. The Alpha System revealed significantly less apical straightening compared with the other instruments (P < 0.05; Mann-Whitney U test). In the apical cross-sections Alpha System resulted in significantly less uninstrumented canal walls compared with stainless steel files (P < 0.05; chi-squared test). Despite the demonstrated differences between the systems, an apical straightening effect could not be prevented; areas of uninstrumented root canal wall were left in all regions using the various systems.

Improve the catalytic activity of {alpha}-Fe{sub 2}O{sub 3} particles in decomposition of ammonium perchlorate by coating amorphous carbon on their surface

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang Yifu; Liu Xinghai, E-mail: liuxh@whu.edu.c; Nie Jiaorong

2011-02-15

Sphere- and pod-like {alpha}-Fe{sub 2}O{sub 3} particles have been selectively synthesized using NH{sub 3}.H{sub 2}O and NaOH solution to adjust the pH value of the designed synthetic system, respectively. The sphere-like {alpha}-Fe{sub 2}O{sub 3} particles with diameter about 25 nm on average were encapsulated into carbon shells to fabricate a novel core-shell composite ({alpha}-Fe{sub 2}O{sub 3}-C) through the coating experiments. The catalytic performance of the products on the thermal decomposition of ammonium perchlorate (AP) was investigated by thermal gravimetric analyzer (TG) and differential thermal analysis (DTA). The thermal decomposition temperatures of AP in the presence of pod-like {alpha}-Fe{sub 2}O{sub 3},more » sphere-like {alpha}-Fe{sub 2}O{sub 3} and {alpha}-Fe{sub 2}O{sub 3}-C are reduced by 72, 81 and 109 {sup o}C, respectively, which show that {alpha}-Fe{sub 2}O{sub 3}-C core-shell composites have higher catalytic activity than that of {alpha}-Fe{sub 2}O{sub 3}. -- Graphical abstract: The catalytic performance of pod-like {alpha}-Fe{sub 2}O{sub 3}, sphere-like {alpha}-Fe{sub 2}O{sub 3} and {alpha}-Fe{sub 2}O{sub 3}-C on the thermal decomposition of ammonium perchlorate (AP). Display Omitted Research highlights: {yields} Sphere- and pod-like {alpha}-Fe{sub 2}O{sub 3} particles have been selectively synthesized using NH{sub 3}.H{sub 2}O and NaOH solution to adjust the pH value. {yields} A novel core-shell composite ({alpha}-Fe{sub 2}O{sub 3}-C core-shell structured composite) has been successfully synthesized using sphere-like {alpha}-Fe{sub 2}O{sub 3} particles as the cores and glucose as the source of carbon. {yields} The thermal decomposition temperatures of AP in the presence of pod-like {alpha}-Fe{sub 2}O{sub 3}, sphere-like {alpha}-Fe{sub 2}O{sub 3} and {alpha}-Fe{sub 2}O{sub 3}-C are reduced by 72, 81 and 109 {sup o}C, respectively, which shows that these materials have high catalytic activity.« less

Inflammatory C-reactive protein and cytokine levels in asymptomatic people with chronic spinal cord injury.

PubMed

Frost, Frederick; Roach, Mary Jo; Kushner, Irving; Schreiber, Peter

2005-02-01

To determine the relation between serologic markers of information and clinical characteristics of people with chronic spinal cord injury (SCI). Cross-sectional study. Academic medical center SCI outpatient clinic. Convenience sample of 37 men with chronic SCI and 10 healthy control subjects. Not applicable. Serum levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and C-reactive protein (CRP). The following results achieved statistical significance at P less than .05. Asymptomatic chronic SCI patients differed from referent controls with respect to serum CRP levels but not IL-6 or TNF-alpha. In SCI patients, higher levels of CRP correlated negatively with hemoglobin and albumin levels. A longer time since injury correlated with lower TNF-alpha values, whereas higher TNF-alpha levels correlated with higher serum albumin. Pressure ulcers and indwelling urinary catheters were associated with higher mean levels of CRP but not of the cytokines TNF-alpha and IL-6. Intermittent urinary catheterization was associated with lower levels of CRP when compared with other methods of bladder management. Asymptomatic people with long-term SCI, especially those with indwelling urinary catheters, showed serologic evidence of a systemic inflammatory state. There was no evidence of an elevation in proinflammatory cytokines. Detection of an ongoing systemic inflammatory response in apparently healthy people with indwelling urinary catheters and small skin ulcers further supports the aggressive pursuit of catheter-free voiding options and pressure ulcer healing.

Effect of clonidine on ultrasonic vocalization in preweaning rats.

PubMed

Hård, E; Engel, J; Lindh, A S

1988-01-01

The present study was undertaken to investigate the involvement of the noradrenergic neurotransmission system in the ultra sonic callings emitted by rat pups separated from their mother and exposed to cold stimulation. The investigation was primarily performed by help of agents selectively affecting the alpha-adrenoceptors: the alpha 2-agonist clonidine, the alpha 1-antagonist prazosin and the alpha 2-antagonist idazoxan. Clonidine dose-dependently stimulated the amount of ultra sonic vocalization, an effect not solely dependent upon the effect of clonidine on body temperature. In a developmental study it was found that clonidine uniformly stimulated crying at all ages from 4 days of age up to 18 days of age, that is during the whole preweaning period. Clonidine stimulated ultrasonic crying in rat pups, devoid of presynaptic catecholamine (CA) neurons by combined pretreatment with the monoamine depletor, reserpine, and the inhibitor of CA-synthesis, alpha-methyl-tyrosine. This finding suggested that the stimulating effect of clonidine on ultrasonic vocalization was mediated by postsynaptic adrenoceptors. In pups, 12 days of age, idazoxan blocked the effect of cold stimulation on ultra sonic crying, suggesting that alpha 2-adrenoceptors, presumably postsynaptic ones, mediated this kind of stimulation. Idazoxan also antagonized the effect of clonidine, but only at a dose effective also in control pups. Prazosin had no effect on cold-stimulated crying, but antagonized the effect of clonidine, suggesting that the effect of clonidine was also mediated by alpha 1-receptors. At 18 days of age, prazosin no longer antagonized the effect of clonidine, whereas the antagonizing action of idazoxan was reinforced. The age-dependent variation in responsiveness to the adrenergic drugs suggest maturational changes in the function of the CA-system occurring between 12-16 days of age.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Veeraraghavan, Malathi

This report describes our accomplishments and activities for the project titled Terabit-Scale Hybrid Networking. The key accomplishment is that we developed, tested and deployed an Alpha Flow Characterization System (AFCS) in ESnet. It is being run in production mode since Sept. 2015. Also, a new QoS class was added to ESnet5 to support alpha flows.

An X-ray fluorescence spectrometer and its applications in materials studies

NASA Technical Reports Server (NTRS)

Singh, J. J.; Han, K. S.

1977-01-01

An X-ray fluorescence system based on a Co(57) gamma-ray source has been developed. The system was used to calculate the atomic percentages of iron implanted in titanium targets. Measured intensities of Fe (k-alpha + k-beta) and Ti (k-alpha + k-beta) X-rays from the Fe-Ti targets are in good agreement with the calculated values based on photoelectric cross sections of Ti and Fe for the Co(57) gamma rays.

Functions of fukutin, a gene responsible for Fukuyama type congenital muscular dystrophy, in neuromuscular system and other somatic organs.

PubMed

Yamamoto, Tomoko; Shibata, Noriyuki; Saito, Yoshiaki; Osawa, Makiko; Kobayashi, Makio

2010-06-01

Fukuyama type congenital muscular dystrophy (FCMD) is an autosomal recessive disease, exhibiting muscular dystrophy, and central nervous system (CNS) and ocular malformations. It is included in alpha-dystroglycanopathy, a group of muscular dystrophy showing reduced glycosylation of alpha-dystroglycan. alpha-Dystroglycan is one of the components of dystrophin-glycoprotein complex linking extracellular and intracellular proteins. The sugar chains of alpha-dystroglycan are receptors for extracellular matrix proteins such as laminin. Fukutin, a gene responsible for FCMD, is presumably related to the glycosylation of alpha-dystroglycan like other causative genes of alpha-dystroglycanopathy. The CNS lesion of FCMD is characterized by cobblestone lissencephaly, associated with decreased glycosylation of alpha-dystroglycan in the glia limitans where the basement membrane is formed. Astrocytes whose endfeet form the glia limitans seem to be greatly involved in the genesis of the CNS lesion. Fukutin is probably necessary for astrocytic function. Other components of the CNS may also need fukutin, such as migration and synaptic function in neurons. However, roles of fukutin in oligodendroglia, microglia, leptomeninges and capillaries are unknown at present. Fukutin is expressed in various somatic organs as well, and appears to work differently between epithelial cells and astrocytes. In the molecular level, since the dystrophin-glycoprotein complex is linked to cell signaling pathways involving c-src and c-jun, fukutin may be able to affect cell proliferation/survival. Fukutin was localized in the nucleus on cancer cell lines. With the consideration that mutations of fukutin give rise to wide spectrum of the clinical phenotype, more unknown functions of fukutin besides the glycosylation of alpha-dystroglycan can be suggested. Trials for novel treatments including gene therapy are in progress in muscular dystrophies. Toward effective therapies with minimal side effects, precise evaluation of the pathomechanism of FCMD and the function of fukutin would be required.

Validation of the F-18 high alpha research vehicle flight control and avionics systems modifications

NASA Technical Reports Server (NTRS)

Chacon, Vince; Pahle, Joseph W.; Regenie, Victoria A.

1990-01-01

The verification and validation process is a critical portion of the development of a flight system. Verification, the steps taken to assure the system meets the design specification, has become a reasonably understood and straightforward process. Validation is the method used to ensure that the system design meets the needs of the project. As systems become more integrated and more critical in their functions, the validation process becomes more complex and important. The tests, tools, and techniques which are being used for the validation of the high alpha research vehicle (HARV) turning valve control system (TVCS) are discussed, and their solutions are documented. The emphasis of this paper is on the validation of integrated systems.

Validation of the F-18 high alpha research vehicle flight control and avionics systems modifications

NASA Technical Reports Server (NTRS)

Chacon, Vince; Pahle, Joseph W.; Regenie, Victoria A.

1990-01-01

The verification and validation process is a critical portion of the development of a flight system. Verification, the steps taken to assure the system meets the design specification, has become a reasonably understood and straightforward process. Validation is the method used to ensure that the system design meets the needs of the project. As systems become more integrated and more critical in their functions, the validation process becomes more complex and important. The tests, tools, and techniques which are being used for the validation of the high alpha research vehicle (HARV) turning vane control system (TVCS) are discussed and the problems and their solutions are documented. The emphasis of this paper is on the validation of integrated system.

PREVAIL-EPL alpha tool electron optics subsystem

NASA Astrophysics Data System (ADS)

Pfeiffer, Hans C.; Dhaliwal, Rajinder S.; Golladay, Steven D.; Doran, Samuel K.; Gordon, Michael S.; Kendall, Rodney A.; Lieberman, Jon E.; Pinckney, David J.; Quickle, Robert J.; Robinson, Christopher F.; Rockrohr, James D.; Stickel, Werner; Tressler, Eileen V.

2001-08-01

The IBM/Nikon alliance is continuing pursuit of an EPL stepper alpha tool based on the PREVAIL technology. This paper provides a status report of the alliance activity with particular focus on the Electron Optical Subsystem developed at IBM. We have previously reported on design features of the PREVAIL alpha system. The new state-of-the-art e-beam lithography concepts have since been reduced to practice and turned into functional building blocks of a production level lithography tool. The electron optical alpha tool subsystem has been designed, build, assembled and tested at IBM's Semiconductor Research and Development Center (SRDC) in East Fishkill, New York. After demonstrating subsystem functionality, the electron optical column and all associated control electronics hardware and software have been shipped during January 2001 to Nikon's facility in Kumagaya, Japan, for integration into the Nikon commercial e-beam stepper alpha tool. Early pre-shipment results obtained with this electron optical subsystem are presented.

IUE detection of bursts of H Ly-alpha emission from Saturn

NASA Technical Reports Server (NTRS)

Clarke, J. T.; Moos, H. W.; Atreya, S. K.; Lane, A. L.

1981-01-01

A new investigation is reported of the potential sources of Ly-alpha emission in a series of observations of the Saturnian system carried out between January and July 1980 using the short wavelength spectrograph of the IUE Observatory. It is noted that north-south maps of the Ly-alpha emission across the planet disk show pronounced spatial asymmetries in emission brightness. These asymmetries vary to a marked extent on a time scale of days and are interpreted as bursts of Ly-alpha emission of as much as 1 kR brightness averaged over a 6 x 10 arcsec area, above a constant planetary emission level of 700-800 R. In fact, the Ly-alpha emission peaks manifest themselves as essentially point source features in these data; it is pointed out that if the emitting region is smaller than the 6 x 10 arcsec instrumental resolution, the surface brightness must be proportionally higher.

The fibroblast growth factor receptor (FGFR) agonist FGF1 and the neural cell adhesion molecule-derived peptide FGL activate FGFR substrate 2alpha differently.

PubMed

Chen, Yongshuo; Li, Shizhong; Berezin, Vladimir; Bock, Elisabeth

2010-07-01

Activation of fibroblast growth factor (FGF) receptors (FGFRs) both by FGFs and by the neural cell adhesion molecule (NCAM) is crucial in the development and function of the nervous system. We found that FGFR substrate 2alpha (FRS2alpha), Src homologous and collagen A (ShcA), and phospholipase-Cgamma (PLCgamma) were all required for neurite outgrowth from cerebellar granule neurons (CGNs) induced by FGF1 and FGL (an NCAM-derived peptide agonist of FGFR1). Like FGF1, FGL induced tyrosine phosphorylation of FGFR1, FRS2alpha, ShcA, and PLCgamma in a time- and dose-dependent manner. However, the activation of FRS2alpha by FGL was significantly lower than the activation by FGF1, indicating a differential signaling profile induced by NCAM compared with the cognate growth factor.

Tocopherol production in plant cell cultures.

PubMed

Caretto, Sofia; Nisi, Rossella; Paradiso, Annalisa; De Gara, Laura

2010-05-01

Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, essential dietary components for mammals and exclusively synthesized by photosynthetic organisms. Of the four forms (alpha, beta, gamma and delta), alpha-tocopherol is the major vitamin E form present in green plant tissues, and has the highest vitamin E activity. Synthetic alpha-tocopherol, being a racemic mixture of eight different stereoisomers, always results less effective than the natural form (R,R,R) alpha-tocopherol. This raises interest in obtaining this molecule from natural sources, such as plant cell cultures. Plant cell and tissue cultures are able to produce and accumulate valuable metabolites that can be used as food additives, nutraceuticals and pharmaceuticals. Sunflower cell cultures, growing under heterotrophic conditions, were exploited to establish a suitable in vitro production system of natural alpha-tocopherol. Optimization of culture conditions, precursor feeding and elicitor application were used to improve the tocopherol yields of these cultures. Furthermore, these cell cultures were useful to investigate the relationship between alpha-tocopherol biosynthesis and photomixotrophic culture conditions, revealing the possibility to enhance tocopherol production by favouring sunflower cell photosynthetic properties. The modulation of alpha-tocopherol levels in plant cell cultures can provide useful hints for a regulatory impact on tocopherol metabolism.

Periplasmic expression of human interferon-alpha 2c in Escherichia coli results in a correctly folded molecule.

PubMed Central

Voss, T; Falkner, E; Ahorn, H; Krystek, E; Maurer-Fogy, I; Bodo, G; Hauptmann, R

1994-01-01

Human interferon-alpha 2c (IFN-alpha 2c) was produced in Escherichia coli under the control of the alkaline phosphatase promoter using a periplasmic expression system. Compared with other leader sequences, the heat-stable enterotoxin II leader of E. coli (STII) resulted in the highest rate of correct processing as judged by Western-blot analysis. The fermentation was designed as a batch-fed process in order to obtain a high yield of biomass. The processing rate of IFN-alpha 2c could be increased from 25% to more than 50% by shifting the fermentation pH from 7.0 to 6.7. IFN-alpha 2c extracted from the periplasm was purified by a new four-step chromatographic procedure. Whereas cytoplasmically produced IFN-alpha 2c does not have its full native structure, IFN-alpha 2c extracted from the periplasm was found to be correctly folded, as shown by c.d. spectroscopy. Peptide-map analysis in combination with m.s. revealed the correct formation of disulphide bridges. N-terminal sequence analysis showed complete removal of the leader sequence, creating the authentic N-terminus starting with cysteine. Images Figure 3 Figure 4 Figure 6 PMID:8141788

Nuclear Track Detector Characterization via Alpha-Spectrometry for Radioprotection Use

DOE Office of Scientific and Technical Information (OSTI.GOV)

Morelli, D.; Imme, G.; Catalano, R.

2011-12-13

Solid Nuclear Track Detectors (SNTDs), CR-39 type, are usually adopted to monitor radon gas concentrations. In order to characterize the detectors according to track geometrical parameters, detectors were irradiated inside a vacuum chamber by alpha particles at twelve energy values, obtained by different Mylar foils in front of a {sup 241}Am source. The alpha energy values were verified using a Si detector. After the exposure to the alpha particles, the detectors were chemically etched to enlarge the tracks, which were then analyzed by means of a semiautomatic system composed of an optical microscope equipped with a CCD camera connected tomore » a personal computer to store images. A suitable routine analyzed the track parameters: major and minor axis length and mean grey level, allowing us to differentiate tracks according to the incident alpha energy and then to individuate the discrimination factors for radon alpha tracks. The combined use of geometrical and optical parameters allows one to overcome the ambiguity in the alpha energy determination due to the non-monotonicity of each parameter versus energy. After track parameter determination, a calibration procedure was performed by means of a radon chamber. The calibration was verified through an inter-comparing survey.« less

Ab initio electronic structure of the progestogen norethisterone and its 5 alpha-derivatives.

PubMed

Kubli-Garfias, Carlos; Vázquez, Ricardo; Cooney, Austin J; Larrea, Fernando

2002-11-01

The steroid 17 alpha-ethynyl-19-nor-4-androsten-17 beta-ol, 3-one (Norethisterone; NET) and its 5 alpha-dihydro (5 alpha-NET), 3 alpha- and 3 beta-tetrahydro derivatives (3 alpha,5 alpha- and 3 beta,5 alpha-NET), were comparatively studied by the ab initio quantum mechanics theory. Additionally, 5 alpha-androstan-3 beta,17 beta-diol (ADIOL) was also studied. The Hartree-Fock method and the 6-31G(*) basis set were used to obtain the lowest energy conformation, geometries, electronic structure and physicochemical properties of the steroids. The results showed bond distances and valence angles similar among all steroids, but some differences in dihedral angles in the A-B-ring system were observed. The electronic structure analysis showed that NET has both frontier orbitals that is, the highest occupied molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO) located at the C4-C5 pi-bond. In A-ring reduced derivatives, the HOMO was found at the 17 beta-OH and ethynyl groups. In the case of 5 alpha-NET, the LUMO was confined to the A-ring and its C3 carbonyl group while the two NET tetrahydro-reduced derivatives showed the LUMO at the 17 beta-OH and ethynyl groups. The energy changes of the rotational barrier of the 17 beta-OH group suggest that its movement is somewhat restricted by the 17 alpha-ethynyl group. Interestingly both groups at C17 form a single electrostatic potential with high electronic density. On the other side, the 19-nor condition increases the A-ring mobility. However, the 3 beta-OH group of 3 beta,5 alpha-NET may rotate without significant energy differences as compared to the same group in ADIOL. The electronic structure of NET and its A-ring reduced derivatives explains in some extent their interaction with androgen and progesterone receptors as well as their selectivity for the estrogen alpha-receptor.

Interaction between alpha 2-adrenergic and angiotensin II systems in the control of glomerular hemodynamics as assessed by renal micropuncture in the rat

NASA Technical Reports Server (NTRS)

Thomson, S. C.; Gabbai, F. B.; Tucker, B. J.; Blantz, R. C.

1992-01-01

The hypothesis that renal alpha 2 adrenoceptors influence nephron filtration rate (SNGFR) via interaction with angiotensin II (AII) was tested by renal micropuncture. The physical determinants of SNGFR were assessed in adult male Munich Wistar rats 5-7 d after ipsilateral surgical renal denervation (DNX). DNX was performed to isolate inhibitory central and presynaptic alpha 2 adrenoceptors from end-organ receptors within the kidney. Two experimental protocols were employed: one to test whether prior AII receptor blockade with saralasin would alter the glomerular hemodynamic response to alpha 2 adrenoceptor stimulation with the selective agonist B-HT 933 under euvolemic conditions, and the other to test whether B-HT 933 would alter the response to exogenous AII under conditions of plasma volume expansion. In euvolemic rats, B-HT 933 caused SNGFR to decline as the result of a decrease in glomerular ultrafiltration coefficient (LpA), an effect that was blocked by saralasin. After plasma volume expansion, B-HT 933 showed no primary effect on LpA but heightened the response of arterial blood pressure, glomerular transcapillary pressure gradient, and LpA to AII. The parallel results of these converse experiments suggest a complementary interaction between renal alpha 2-adrenergic and AII systems in the control of LpA.

Reproduction-associated immunoreactive peptides in the nervous systems of prosobranch gastropods.

PubMed

Ram, J L; Gallardo, C S; Ram, M L; Croll, R P

1998-12-01

Antibodies against reproductive peptides of Aplysia and Lymnaea were used to localize homologous immunoreactive peptides in the nervous systems of three prosobranch species: Busycon canaliculatum, Concholepas concholepas, and Tegula atra. Positive control experiments in L. stagnalis demonstrated the broad species range of the anti-egg-laying hormone (anti-ELH) antibody used in this study, and showed binding of anti-alpha-caudodorsal-cell peptide (anti-alpha-CDCP) to the same cells in cerebral and buccal ganglia. Dot immunoassays with synthetic ELH confirmed the reactivity and sensitivity (< 0.1 microgram) of the anti-ELH antibody. Experiments with preadsorbed antibody or no primary antibody confirmed its specificity. In B. canaliculatum, clusters of more than 300 neuronal cell bodies immunoreactive to both anti-ELH and anti-alpha-CDCP were observed along the medial margins of left and right cerebral ganglia. Anti-alpha-CDCP reacted with additional small populations of cerebral ganglion neurons not stained by anti-ELH. Anti-ELH and anti-alpha-CDCP also reacted with overlapping but different small populations of neurons in buccal ganglia. In C. concholepas and T atra, ELH-like immunoreactivity was found in cerebral ganglia, and in T. atra in fibers in the cerebral ganglia and cerebral-pedal connectives. Thus, cerebral ganglia are the major locus of the ELH-like immunoreactivity in prosobranchs.

The X-ray Crystal Structures of Human {alpha}-Phosphomannomutase 1 Reveal the Structural Basis of Congenital Disorder of Glycosylation Type 1a

DOE Office of Scientific and Technical Information (OSTI.GOV)

Silvaggi,N.; Zhang, C.; Lu, Z.

2006-01-01

Carbohydrate-deficient glycoprotein syndrome type 1a (CDG-1a) is a congenital disease characterized by severe defects in nervous system development. It is caused by mutations in alpha -phosphomannomutase (of which there are two isozymes, {alpha}-PMM1 and {alpha}-PPM2). Here we report the X-ray crystal structures of human {alpha}-PMM1 in the open conformation, with and without the bound substrate, {alpha}-D-mannose 1-phosphate. {alpha}-PMM1, like most Haloalkanoic Acid Dehalogenase Superfamily (HADSF) members, consists of two domains, the cap and core, which open to bind substrate and then close to provide a solvent exclusive environment for catalysis. The substrate phosphate group is observed at a positively chargedmore » site of the cap domain, rather than at the core domain phosphoryl-transfer site defined by the D19 nucleophile and Mg{sup 2+} cofactor. This suggests that substrate binds first to the cap and then is swept into the active site upon cap closure. The orientation of the acid/base residue D21 suggests that {alpha}-PMM uses a different method of protecting the aspartylphosphate from hydrolysis than the HADSF member {beta}-phosphoglucomutase. It is hypothesized that the electrostatic repulsion of positive charges at the interface of the cap and core domains stabilizes {alpha}-PMM1 in the open conformation, and that the negatively charged substrate binds to the cap, thereby facilitating its closure over the core domain. The two isozymes {alpha}-PMM1 and {alpha}-PMM2 are shown to have a conserved active-site structure and to display similar kinetic properties. Analysis of the known mutation sites in the context of the structures reveals the genotype-phenotype relationship underlying CDG-1a.« less

Density-induced suppression of the {alpha}-particle condensate in nuclear matter and the structure of {alpha}-cluster states in nuclei

DOE Office of Scientific and Technical Information (OSTI.GOV)

Funaki, Y.; Horiuchi, H.; International Institute for Advanced Studies, Kizugawa 619-0225

2008-06-15

At low densities, with decreasing temperatures, in symmetric nuclear matter {alpha} particles are formed, which eventually give raise to a quantum condensate with four-nucleon {alpha}-like correlations (quartetting). Starting with a model of {alpha} matter, where undistorted {alpha} particles interact via an effective interaction such as the Ali-Bodmer potential, the suppression of the condensate fraction at zero temperature with increasing density is considered. Using a Jastrow-Feenberg approach, it is found that the condensate fraction vanishes near saturation density. Additionally, the modification of the internal state of the {alpha} particle due to medium effects will further reduce the condensate. In finite systems,more » an enhancement of the S-state wave function of the center-of-mass orbital of {alpha}-particle motion is considered as the correspondence to the condensate. Wave functions have been constructed for self-conjugate 4n nuclei that describe the condensate state but are fully antisymmetrized on the nucleonic level. These condensate-like cluster wave functions have been successfully applied to describe properties of low-density states near the n{alpha} threshold. Comparison with orthogonality condition model calculations in {sup 12}C and {sup 16}O shows strong enhancement of the occupation of the S-state center-of-mass orbital of the {alpha} particles. This enhancement is decreasing if the baryon density increases, similar to the density-induced suppression of the condensate fraction in {alpha} matter. The ground states of {sup 12}C and {sup 16}O show no enhancement at all, thus a quartetting condensate cannot be formed at saturation densities.« less

The theory of QSO absorption line systems and their relationship to the galaxies

NASA Technical Reports Server (NTRS)

Charlton, Jane

1993-01-01

The fundamental goal of this effort is to paint a picture of what the Ly-alpha forest clouds are and how they are distributed in space. Progress during the first phase of this program involved development of the 'Cheshire Cat Model' of Ly-alpha clouds in which systems over a large range of column densities are produced by disks with somewhat smaller column densities than those of normal galaxies. A prediction of the slab model of Ly-alpha clouds was confirmed by a new observational result, and the comparison of models to the new data allowed an estimate of the pressure of the intergalactic medium. This result should be forthcoming in pre-print form within the next month. The various results will now be described in more detail.

Polar solar wind and interstellar wind properties from interplanetary Lyman-alpha radiation measurements

NASA Technical Reports Server (NTRS)

Witt, N.; Blum, P. W.; Ajello, J. M.

1981-01-01

The analysis of Mariner 10 observations of Lyman-alpha resonance radiation shows an increase of interplanetary neutral hydrogen densities above the solar poles. This increase is caused by a latitudinal variation of the solar wind velocity and/or flux. Using both the Mariner 10 results and other solar wind observations, the values of the solar wind flux and velocity with latitude are determined for several cases of interest. The latitudinal variation of interplanetary hydrogen gas, arising from the solar wind latitudinal variation, is shown to be most pronounced in the inner solar system. From this result it is shown that spacecraft Lyman-alpha observations are more sensitive to the latitudinal anisotropy for a spacecraft location in the inner solar system near the downwind axis.

Shikonins, phytocompounds from Lithospermum erythrorhizon, inhibit the transcriptional activation of human tumor necrosis factor alpha promoter in vivo.

PubMed

Staniforth, Vanisree; Wang, Sheng-Yang; Shyur, Lie-Fen; Yang, Ning-Sun

2004-02-13

Tumor necrosis factor alpha (TNF-alpha) contributes to the pathogenesis of both acute and chronic inflammatory diseases and has been a target for the development of new anti-inflammatory drugs. Shikonins, the naphthoquinone pigments present in the root tissues of Lithospermum erythrorhizon Sieb. et Zucc. (Boraginaceae), have been reported to exert anti-inflammatory effects both in vitro and in vivo. In this study, we evaluated the effects of shikonin and its derivatives on the transcriptional activation of human TNF-alpha promoter in a gene gun-transfected mouse skin system by using a luciferase reporter gene assay. The crude plant extract of L. erythrorhizon as well as derived individual compounds shikonin, isobutyryl shikonin, acetyl shikonin, dimethylacryl shikonin and isovaleryl shikonin showed significant dose-dependent inhibition of TNF-alpha promoter activation. Among the tested compounds, shikonin and isobutyryl shikonin exhibited the highest inhibition of TNF-alpha promoter activation and also showed significant suppression of transgenic human TNF-alpha mRNA expression and protein production. We demonstrated that shikonin-inhibitory response was retained in the core TNF-alpha promoter region containing the TATA box and a 48-bp downstream sequence relative to the transcription start site. Further our results indicated that shikonin suppressed the basal transcription and activator-regulated transcription of TNF-alpha by inhibiting the binding of transcription factor IID protein complex (TATA box-binding protein) to TATA box. These in vivo results suggest that shikonins inhibit the transcriptional activation of the human TNF-alpha promoter through interference with the basal transcription machinery. Thus, shikonins may have clinical potential as anti-inflammatory therapeutics.

Effects of alpha/beta-androstenediol immune regulating hormones on bone remodeling and apoptosis in osteoblasts.

PubMed

Urban, Nicole H; Chamberlin, Brett; Ramage, Samuel; Roberts, Zachary; Loria, Roger M; Beckman, Matthew J

2008-06-01

A large body of evidence suggests that the immune system directly impacts bone physiology. We tested whether immune regulating hormones (IRH), 17beta-androstenediol (beta-AED), 7beta,17beta-androstenetriol (beta-AET) or the 17alpha-androstenediol (alpha-AED), and 7alpha,17beta-androstenetriol (alpha-AET) metabolites could directly influence bone remodeling in vitro using human fetal osteoblasts (FOB-9). The impact on bone remodeling was examined by comparing the ratio of RANKL/OPG gene expression in response to AED and AET compounds. The alpha-AED was found to significantly increase in the ratio of RANKL/OPG gene expression and altering the morphology of RANKL stained FOB-9 cells. Cell viability was assessed using a Live/Dead assay. Again alpha-AED was unique in its ability to reduce the proportion of viable cells, and to induce mild apoptosis of FOB-9 cells. Treatment of FOB-9 cells with WY14643, an activator of PPAR-alpha and -gamma, also significantly elevated the percentage of dead cells. This increase was abolished by co-treatment with GW9962, a specific inhibitor of PPAR-gamma. Analysis of PPAR-gamma mRNA by Quantitative RT-PCR and its activation by DNA binding demonstrated that alpha-AED increased PPAR-gamma activation by 19%, while beta-AED conferred a 37% decrease in PPAR-gamma activation. In conclusion, alpha-AED opposed beta-AED by elevating a bone resorption scenario in osteoblast cells. The increase in RANKL/OPG is modulated by an activation of PPAR-gamma that in turn caused mild apoptosis of FOB-9 cells.

U-shaped Relation between Prestimulus Alpha-band and Poststimulus Gamma-band Power in Temporal Tactile Perception in the Human Somatosensory Cortex.

PubMed

Wittenberg, Marc André; Baumgarten, Thomas J; Schnitzler, Alfons; Lange, Joachim

2018-04-01

Neuronal oscillations are a ubiquitous phenomenon in the human nervous system. Alpha-band oscillations (8-12 Hz) have been shown to correlate negatively with attention and performance, whereas gamma-band oscillations (40-150 Hz) correlate positively. Here, we studied the relation between prestimulus alpha-band power and poststimulus gamma-band power in a suprathreshold tactile discrimination task. Participants received two electrical stimuli to their left index finger with different SOAs (0 msec, 100 msec, intermediate SOA, intermediate SOA ± 10 msec). The intermediate SOA was individually determined so that stimulation was bistable, and participants perceived one stimulus in half of the trials and two stimuli in the other half. We measured neuronal activity with magnetoencephalography (MEG). In trials with intermediate SOAs, behavioral performance correlated inversely with prestimulus alpha-band power but did not correlate with poststimulus gamma-band power. Poststimulus gamma-band power was high in trials with low and high prestimulus alpha-band power and low for intermediate prestimulus alpha-band power (i.e., U-shaped). We suggest that prestimulus alpha activity modulates poststimulus gamma activity and subsequent perception: (1) low prestimulus alpha-band power leads to high poststimulus gamma-band power, biasing perception such that two stimuli were perceived; (2) intermediate prestimulus alpha-band power leads to low gamma-band power (interpreted as inefficient stimulus processing), consequently, perception was not biased in either direction; and (3) high prestimulus alpha-band power leads to high poststimulus gamma-band power, biasing perception such that only one stimulus was perceived.

Development of the prototype data management system of the solar H-alpha full disk observation

NASA Astrophysics Data System (ADS)

Wei, Ka-Ning; Zhao, Shi-Qing; Li, Qiong-Ying; Chen, Dong

2004-06-01

The Solar Chromospheric Telescope in Yunnan Observatory generates about 2G bytes fits format data per day. Huge amounts of data will bring inconvenience for people to use. Hence, data searching and sharing are important at present. Data searching, on-line browsing, remote accesses and download are developed with a prototype data management system of the solar H-alpha full disk observation, and improved by the working flow technology. Based on Windows XP operating system and MySQL data management system, a prototype system of browse/server model is developed by JAVA and JSP. Data compression, searching, browsing, deletion need authority and download in real-time have been achieved.

A Search for Transits of Proxima b in MOST Photometry

NASA Astrophysics Data System (ADS)

Kipping, David M.

2017-01-01

The recent discovery of a potentially rocky planet in the habitable-zone of our nearest star presents exciting prospects for future detailed characterization of another world. If Proxima b transits its star, the road to characterization would be considerably eased. In 2014 and 2015, we monitored Proxima Centauri with the Canadian space telescope MOST for a total of 43 days. As expected, the star presents considerable photometric variability due to flares, which greatly complicate our analysis. Using Gaussian process regression and Bayesian model selection with informative priors for the time of transit of Proxima b, we do find evidence for a transit of the expected depth. However, relaxing the prior on the transit time to an uninformative one returns a distinct solution highlighting the high false-positive rate induced by flaring. Using ground-based photometry from HATSouth, we show that our candidate transit is unlikely to be genuine although a conclusive answer will likely require infrared photometry, such as that from Spitzer, where flaring should be suppressed.

Unidentified emission features in the R Coronae Borealis star V854 Centauri

NASA Astrophysics Data System (ADS)

Oostrum, L. C.; Ochsendorf, B. B.; Kaper, L.; Tielens, A. G. G. M.

2018-02-01

During its 2012 decline, the R Coronae Borealis star (RCB) V854 Cen was spectroscopically monitored with X-shooter on the ESO Very Large Telescope. The obscured optical and near-infrared spectrum exhibits many narrow and several broad emission features, as previously observed. The envelope is spatially resolved along the slit and allows for a detailed study of the circumstellar material. In this Letter, we report on the properties of a number of unidentified visual emission features (UFs), including the detection of a new feature at 8692 Å. These UFs have been observed in the Red Rectangle (RR), but their chemical and physical nature is still a mystery. The previously known UFs behave similarly in the RR and in V854 Cen, but are not detected in six other observed RCBs. Some hydrogen might be required for the formation of their carrier(s). The λ8692 UF is present in all RCBs. Its carrier is likely of a carbonaceous molecular nature, presumably different from that of the other UFs.

Why the New Gully Deposits are Not Dry Dust Slope Streaks

NASA Image and Video Library

2006-12-06

The light-toned deposits that formed in two gully sites on Mars during the Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) mission in the 1999 to 2005 period are considered to be the result of sediment transport by a fluid with the physical properties of liquid water. The young, light-toned gully deposits were found in a crater in Terra Sirenum (see PIA09027 or MOC2-1618) and in a crater east of the Hellas basin in the Centauri Montes region (see PIA09028 or MOC2-1619). In their study of how the light-toned gully deposits may have formed, the MOC team considered their resemblance to light- and dark-toned slope streaks found elsewhere on Mars. Slope streaks are most commonly believed to have formed by downslope movement of extremely dry, very fine-grained dust, through processes thought by some to be analogous to terrestrial snow avalanche formation. http://photojournal.jpl.nasa.gov/catalog/PIA09030

A stellar audit: the computation of encounter rates for 47 Tucanae and omega Centauri

NASA Astrophysics Data System (ADS)

Davies, Melvyn B.; Benz, Willy

1995-10-01

Using King-Mitchie models, we compute encounter rates between the various stellar species in the globular clusters omega Cen and 47 Tuc. We also compute event rates for encounters between single stars and a population of primordial binaries. Using these rates, and what we have learnt from hydrodynamical simulations of encounters performed earlier, we compute the production rates of objects such as low-mass X-ray binaries (LMXBs), smothered neutron stars and blue stragglers (massive main-sequence stars). If 10 per cent of the stars are contained in primordial binaries, the production rate of interesting objects from encounters involving these binaries is as large as that from encounters between single stars. For example, encounters involving binaries produce a significant number of blue stragglers in both globular cluster models. The number of smothered neutron stars may exceed the number of LMXBs by a factor of 5-20, which may help to explain why millisecond pulsars are observed to outnumber LMXBs in globular clusters.

The ultraviolet-bright stars of Omega Centauri, M3, and M13

NASA Technical Reports Server (NTRS)

Landsman, Wayne B.; O'Connell, Robert W.; Whitney, Jonathan H.; Bohlin, Ralph C.; Hill, Robert S.; Maran, Stephen P.; Parise, Ronald A.; Roberts, Morton S.; Smith, Andrew A.; Stecher, Theodore P.

1992-01-01

Two new UV-bright stars detected within 2 arcmin of the center of Omega Cen are spectroscopically investigated with the short-wavelength spectrograph of the IUE. The IUE spectra of the UV-bright stars UIT-1 and UIT-2 in the core of Omega Cen superficially resemble those of Population I mid-B stars. The absorption lines of the core UV-bright stars are significantly weaker than in Population I stars, consistent with their membership in the cluster. Synthetic spectra calculated from low-metallicity Kurucz model stellar atmospheres are compared with the spectra. These objects are insufficiently luminous to be classical hydrogen-burning post-AGB stars. They may be evolved hot horizontal branch stars which have been brightened by more than 3 mag since leaving the zero-age horizontal branch. It is inferred from the spectra and luminosity of the core UV-bright stars that similar objects could provide the source of the UV light in elliptical galaxies.

Relativistic Gas Drag on Dust Grains and Implications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoang, Thiem, E-mail: thiemhoang@kasi.re.kr; Korea University of Science and Technology, Daejeon, 34113

We study the drag force on grains moving at relativistic velocities through interstellar gas and explore its application. First, we derive a new analytical formula of the drag force at high energies and find that it is significantly reduced compared to the classical model. Second, we apply the obtained drag force to calculate the terminal velocities of interstellar grains by strong radiation sources such as supernovae and active galactic nuclei (AGNs). We find that grains can be accelerated to relativistic velocities by very luminous AGNs. We then quantify the deceleration of relativistic spacecraft proposed by the Breakthrough Starshot initiative duemore » to gas drag on a relativistic lightsail. We find that the spacecraft’s decrease in speed is negligible because of the suppression of gas drag at relativistic velocities, suggesting that the lightsail may be open for communication during its journey to α Centauri without causing a considerable delay. Finally, we show that the damage to relativistic thin lightsails by interstellar dust is a minor effect.« less