Science.gov

Sample records for alternativer til ny

  1. Tumor-infiltrating NY-ESO-1-specific CD8+ T cells are negatively regulated by LAG-3 and PD-1 in human ovarian cancer.

    PubMed

    Matsuzaki, Junko; Gnjatic, Sacha; Mhawech-Fauceglia, Paulette; Beck, Amy; Miller, Austin; Tsuji, Takemasa; Eppolito, Cheryl; Qian, Feng; Lele, Shashikant; Shrikant, Protul; Old, Lloyd J; Odunsi, Kunle

    2010-04-27

    NY-ESO-1 is a "cancer-testis" antigen frequently expressed in epithelial ovarian cancer (EOC) and is among the most immunogenic tumor antigens defined to date. In an effort to understand in vivo tolerance mechanisms, we assessed the phenotype and function of NY-ESO-1-specific CD8(+) T cells derived from peripheral blood lymphocytes (PBLs), tumor-infiltrating lymphocytes (TILs), and tumor-associated lymphocytes (TALs) of EOC patients with NY-ESO-1-expressing tumors, with or without humoral immunity to NY-ESO-1. Whereas NY-ESO-1-specific CD8(+) T cells were readily detectable ex vivo with tetramers in TILs and TALs of seropositive patients, they were only detectable in PBLs following in vitro stimulation. Compared with PBLs, tumor-derived NY-ESO-1-specific CD8(+) T cells demonstrated impaired effector function, preferential usage of dominant T-cell receptor, and enriched coexpression of inhibitory molecules LAG-3 and PD-1. Expression of LAG-3 and PD-1 on CD8(+) T cells was up-regulated by IL-10, IL-6 (cytokines found in tumor ascites), and tumor-derived antigen-presenting cells. Functionally, CD8(+)LAG-3(+)PD-1(+) T cells were more impaired in IFN-gamma/TNF-alpha production compared with LAG-3(+)PD-1(-) or LAG-3(-)PD-1(-) subsets. Dual blockade of LAG-3 and PD-1 during T-cell priming efficiently augmented proliferation and cytokine production by NY-ESO-1-specific CD8(+) T cells, indicating that antitumor function of NY-ESO-1-specific CD8(+) T cells could potentially be improved by therapeutic targeting of these inhibitory receptors.

  2. Ny-Alesund Geodetic Observatory

    NASA Technical Reports Server (NTRS)

    Sieber, Moritz

    2013-01-01

    In 2012 the 20-m telescope at Ny-Alesund, Svalbard, operated by the Norwegian Mapping Authority (NMA), took part in 163 out of 168 scheduled sessions of the IVS program. Since spring, all data was transferred by network, and the receiver monitoring computer was replaced by a bus-coupler. In autumn, the NMA received building permission for a new observatory from the Governor of Svalbard. The bidding process and first construction work for the infrastructure will start in 2013.

  3. 46 CFR 7.25 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Montauk Point, NY to Atlantic Beach, NY. 7.25 Section 7... LINES Atlantic Coast § 7.25 Montauk Point, NY to Atlantic Beach, NY. (a) A line drawn from Shinnecock... Light 348° true to the southernmost extremity of the spit of land at the western end of Oak Beach. (d)...

  4. 46 CFR 7.25 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Montauk Point, NY to Atlantic Beach, NY. 7.25 Section 7... LINES Atlantic Coast § 7.25 Montauk Point, NY to Atlantic Beach, NY. (a) A line drawn from Shinnecock... Light 348° true to the southernmost extremity of the spit of land at the western end of Oak Beach. (d)...

  5. 46 CFR 7.25 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Montauk Point, NY to Atlantic Beach, NY. 7.25 Section 7... LINES Atlantic Coast § 7.25 Montauk Point, NY to Atlantic Beach, NY. (a) A line drawn from Shinnecock... Light 348° true to the southernmost extremity of the spit of land at the western end of Oak Beach. (d)...

  6. 46 CFR 7.25 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Montauk Point, NY to Atlantic Beach, NY. 7.25 Section 7... LINES Atlantic Coast § 7.25 Montauk Point, NY to Atlantic Beach, NY. (a) A line drawn from Shinnecock... Light 348° true to the southernmost extremity of the spit of land at the western end of Oak Beach. (d)...

  7. 46 CFR 7.25 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Montauk Point, NY to Atlantic Beach, NY. 7.25 Section 7... LINES Atlantic Coast § 7.25 Montauk Point, NY to Atlantic Beach, NY. (a) A line drawn from Shinnecock... Light 348° true to the southernmost extremity of the spit of land at the western end of Oak Beach. (d)...

  8. 49 CFR 372.201 - Albany, NY.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... section, thence along the western and northern boundary of Cohoes to the Mohawk River thence along such... 49 Transportation 5 2014-10-01 2014-10-01 false Albany, NY. 372.201 Section 372.201 Transportation... TERMINAL AREAS Commercial Zones § 372.201 Albany, NY. The zone adjacent to, and commercially a part...

  9. 49 CFR 372.201 - Albany, NY.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... section, thence along the western and northern boundary of Cohoes to the Mohawk River thence along such... 49 Transportation 5 2010-10-01 2010-10-01 false Albany, NY. 372.201 Section 372.201 Transportation... TERMINAL AREAS Commercial Zones § 372.201 Albany, NY. The zone adjacent to, and commercially a part...

  10. 49 CFR 372.201 - Albany, NY.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... section, thence along the western and northern boundary of Cohoes to the Mohawk River thence along such... 49 Transportation 5 2011-10-01 2011-10-01 false Albany, NY. 372.201 Section 372.201 Transportation... TERMINAL AREAS Commercial Zones § 372.201 Albany, NY. The zone adjacent to, and commercially a part...

  11. 49 CFR 372.201 - Albany, NY.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... section, thence along the western and northern boundary of Cohoes to the Mohawk River thence along such... 49 Transportation 5 2012-10-01 2012-10-01 false Albany, NY. 372.201 Section 372.201 Transportation... TERMINAL AREAS Commercial Zones § 372.201 Albany, NY. The zone adjacent to, and commercially a part...

  12. 49 CFR 372.201 - Albany, NY.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Albany, N.Y., within which transportation by motor vehicle, in interstate or foreign commerce, not under... combined areas defined in paragraphs (b) and (c) of this section, and (e) All of any municipality...

  13. 33 CFR 80.160 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Beach, NY. 80.160 Section 80.160 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... to Atlantic Beach, NY. (a) A line drawn from the Shinnecock Inlet East Breakwater Light to Shinnecock... southernmost extremity of the spit of land at the western end of Oak Beach. (d) A line drawn from Jones...

  14. 33 CFR 80.160 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Beach, NY. 80.160 Section 80.160 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... to Atlantic Beach, NY. (a) A line drawn from the Shinnecock Inlet East Breakwater Light to Shinnecock... southernmost extremity of the spit of land at the western end of Oak Beach. (d) A line drawn from Jones...

  15. 33 CFR 80.160 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Beach, NY. 80.160 Section 80.160 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... to Atlantic Beach, NY. (a) A line drawn from the Shinnecock Inlet East Breakwater Light to Shinnecock... southernmost extremity of the spit of land at the western end of Oak Beach. (d) A line drawn from Jones...

  16. 33 CFR 80.160 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Beach, NY. 80.160 Section 80.160 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... to Atlantic Beach, NY. (a) A line drawn from the Shinnecock Inlet East Breakwater Light to Shinnecock... southernmost extremity of the spit of land at the western end of Oak Beach. (d) A line drawn from Jones...

  17. 33 CFR 80.160 - Montauk Point, NY to Atlantic Beach, NY.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Beach, NY. 80.160 Section 80.160 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... to Atlantic Beach, NY. (a) A line drawn from the Shinnecock Inlet East Breakwater Light to Shinnecock... southernmost extremity of the spit of land at the western end of Oak Beach. (d) A line drawn from Jones...

  18. 49 CFR 372.235 - New York, NY.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false New York, NY. 372.235 Section 372.235... ZONES, AND TERMINAL AREAS Commercial Zones § 372.235 New York, NY. The zone adjacent to, and commercially a part of, New York, NY, within which transportation by motor vehicle, in interstate or...

  19. 49 CFR 372.235 - New York, NY.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 5 2011-10-01 2011-10-01 false New York, NY. 372.235 Section 372.235... ZONES, AND TERMINAL AREAS Commercial Zones § 372.235 New York, NY. The zone adjacent to, and commercially a part of, New York, NY, within which transportation by motor vehicle, in interstate or...

  20. 49 CFR 372.235 - New York, NY.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 5 2012-10-01 2012-10-01 false New York, NY. 372.235 Section 372.235... ZONES, AND TERMINAL AREAS Commercial Zones § 372.235 New York, NY. The zone adjacent to, and commercially a part of, New York, NY, within which transportation by motor vehicle, in interstate or...

  1. HAER NY,55GRO,1 (sheet 3 of 3) AveryBartholomew Patent Railroad ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    HAER NY,55-GRO,1- (sheet 3 of 3) - Avery-Bartholomew Patent Railroad Iron Bridge, Town park south of Route 222, west of Owasco Inlet (moved from Elm Street Extension spanning Fall Creek, Nubia, NY), Groton, Tompkins County, NY

  2. HAER NY,55GRO,1 (sheet 2 of 3) AveryBartholomew Patent Railroad ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    HAER NY,55-GRO,1- (sheet 2 of 3) - Avery-Bartholomew Patent Railroad Iron Bridge, Town park south of Route 222, west of Owasco Inlet (moved from Elm Street Extension spanning Fall Creek, Nubia, NY), Groton, Tompkins County, NY

  3. HAER NY,55GRO,1 (sheet 1 of 3) AveryBartholomew Patent Railroad ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    HAER NY,55-GRO,1- (sheet 1 of 3) - Avery-Bartholomew Patent Railroad Iron Bridge, Town park south of Route 222, west of Owasco Inlet (moved from Elm Street Extension spanning Fall Creek, Nubia, NY), Groton, Tompkins County, NY

  4. NY-ESO-1 specific antibody and cellular responses in melanoma patients primed with NY-ESO-1 protein in ISCOMATRIX and boosted with recombinant NY-ESO-1 fowlpox virus.

    PubMed

    Chen, Ji-Li; Dawoodji, Amina; Tarlton, Andrea; Gnjatic, Sacha; Tajar, Abdelouahid; Karydis, Ioannis; Browning, Judy; Pratap, Sarah; Verfaille, Christian; Venhaus, Ralph R; Pan, Linda; Altman, Douglas G; Cebon, Jonathan S; Old, Lloyd L; Nathan, Paul; Ottensmeier, Christian; Middleton, Mark; Cerundolo, Vincenzo

    2015-03-15

    Vaccination strategies based on repeated injections of NY-ESO-1 protein formulated in ISCOMATRIX particles (NY-ESO-1 ISCOMATRIX) have shown to elicit combined NY-ESO-1 specific antibody and T cell responses. However, it remains unclear whether heterologous prime-boost strategies based on the combination with NY-ESO-1 ISCOMATRIX with different NY-ESO-1 boosting reagents could be used to increase NY-ESO-1 CD8(+) or CD4(+) T cell responses. To address this question, we carried out a randomized clinical trial in 39 high-risk, resected melanoma patients vaccinated with NY-ESO-1 ISCOMATRIX, and then boosted with repeated injections of either recombinant fowlpox virus encoding full length NY-ESO-1 (rF-NY-ESO-1) (Arm A) or NY-ESO-1 ISCOMATRIX alone (Arm B). We have comprehensively analyzed NY-ESO-1 specific T cells and B cells response in all patients before and after vaccination for a total of seven time points per patient. NY-ESO-1 ISCOMATRIX alone elicited a strong NY-ESO-1 specific CD4(+) T cell and antibody response, which was maintained by both regiments at similar levels. However, CD8(+) T cell responses were significantly boosted in 3 out of 18 patients in Arm A after the first rF-NY-ESO-1 injection and such responses were maintained until the end of the trial, while no patients in Arm B showed similar CD8(+) T cell responses. In addition, our results clearly identified immunodominant regions in the NY-ESO-1 protein: NY-ESO-179-102 and NY-ESO-1115-138 for CD4+ T cells and NY-ESO-185-108 for CD8+ T cells in a large proportion of vaccinated patients. These regions of NY-ESO-1 protein should be considered in future clinical trials as immunodominant epitopes.

  5. 46 CFR 7.30 - New York Harbor, NY.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false New York Harbor, NY. 7.30 Section 7.30 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC BOUNDARY LINES Atlantic Coast § 7.30 New York Harbor, NY. A line drawn from East Rockaway Inlet Breakwater Light to Ambrose...

  6. 46 CFR 7.30 - New York Harbor, NY.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false New York Harbor, NY. 7.30 Section 7.30 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY PROCEDURES APPLICABLE TO THE PUBLIC BOUNDARY LINES Atlantic Coast § 7.30 New York Harbor, NY. A line drawn from East Rockaway Inlet Breakwater Light to Ambrose...

  7. 33 CFR 110.208 - Buffalo Harbor, N.Y.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage...

  8. 33 CFR 110.84b - Buffalo, N.Y.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo, N.Y. 110.84b Section 110.84b Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.84b Buffalo, N.Y. The area within the Port of Buffalo known as...

  9. 33 CFR 110.87 - Henderson Harbor, N.Y.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Henderson Harbor, N.Y. 110.87 Section 110.87 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.87 Henderson Harbor, N.Y. (a) Area A. The area in...

  10. 33 CFR 110.59 - Eastern Long Island, NY.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Eastern Long Island, NY. 110.59... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.59 Eastern Long Island, NY. (a) Huntington Harbor... adjacent to the easterly side of Centre Island, westerly of a line on range with Cold Spring Harbor...

  11. 33 CFR 110.59 - Eastern Long Island, NY.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Eastern Long Island, NY. 110.59... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.59 Eastern Long Island, NY. (a) Huntington Harbor... adjacent to the easterly side of Centre Island, westerly of a line on range with Cold Spring Harbor...

  12. 33 CFR 110.59 - Eastern Long Island, NY.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Eastern Long Island, NY. 110.59... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.59 Eastern Long Island, NY. (a) Huntington Harbor... adjacent to the easterly side of Centre Island, westerly of a line on range with Cold Spring Harbor...

  13. 33 CFR 110.59 - Eastern Long Island, NY.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Eastern Long Island, NY. 110.59... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.59 Eastern Long Island, NY. (a) Huntington Harbor... adjacent to the easterly side of Centre Island, westerly of a line on range with Cold Spring Harbor...

  14. 33 CFR 110.59 - Eastern Long Island, NY.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Eastern Long Island, NY. 110.59... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.59 Eastern Long Island, NY. (a) Huntington Harbor... adjacent to the easterly side of Centre Island, westerly of a line on range with Cold Spring Harbor...

  15. NY 32633 VIEW ALONG WASHINGTON ROAD, LOOKING (RIGHT TO LEFT) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    NY -326-33 VIEW ALONG WASHINGTON ROAD, LOOKING (RIGHT TO LEFT) TOWARD CARPENTER AND BLACKSMITH SHOP (BUILDING 65 I), ORDINANCE OFFICE AND STOREHOUSE (BUILDING 653) AND Q.M. STABLES AND WAGON SHED (BUILDING 657). VIEW TO SOUTHEAST. - Plattsburgh Air Force Base, U.S. Route 9, Plattsburgh, Clinton County, NY

  16. 33 CFR 110.208 - Buffalo Harbor, N.Y.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage...

  17. 33 CFR 110.136 - Lake Champlain, NY and VT.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Lake Champlain, NY and VT. 110.136 Section 110.136 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.136 Lake Champlain, NY and VT. (a) Burlington...

  18. RenewableNY - An Industrial Energy Conservation Initiative

    SciTech Connect

    Lubarr, Tzipora

    2009-09-30

    The New York Industrial Retention Network (NYIRN) manages the RenewableNY program to assist industrial companies in New York City to implement energy efficiency projects. RenewableNY provides companies with project management assistance and grants to identify opportunities for energy savings and implement energy efficiency projects. The program helps companies identify energy efficient projects, complete an energy audit, and connect with energy contractors who install renewable energy and energy efficient equipment. It also provides grants to help cover the costs of installation for new systems and equipment. RenewableNY demonstrates that a small grant program that also provides project management assistance can incentivize companies to implement energy efficiency projects that might otherwise be avoided. Estimated savings through RenewableNY include 324,500 kWh saved through efficiency installations, 158 kW of solar energy systems installed, and 945 thm of gas avoided.

  19. 75 FR 54419 - Environmental Impact Statement: Queens County, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-07

    ... Federal Highway Administration Environmental Impact Statement: Queens County, NY AGENCY: Federal Highway... Environmental Impact Statement (EIS) for the proposed Bridge Rehabilitation and Interchange Improvements Project... considered will not have a significant impact on the environment. To address these bridge conditions,...

  20. 33 CFR 110.84b - Buffalo, N.Y.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Buffalo, N.Y. 110.84b Section 110... REGULATIONS Special Anchorage Areas § 110.84b Buffalo, N.Y. The area within the Port of Buffalo known as Port of Buffalo Small Boat Harbor commencing at a point on shore at latitude 42°51′05″ N., longitude...

  1. Evaluation of TILS for use as the orbiter landing NAVAID

    NASA Technical Reports Server (NTRS)

    Tate, J. M.

    1974-01-01

    An evaluation of the tactical instrument landing systems (TILS) for use in the orbiter autoland system was made. It was found that with certain modifications, the TILS can satisfy orbiter autoland requirements. These modifications, include (1) addition of DME equipment, (2) expansion of elevation coverage from 0-10 deg to 0-30 deg, and (3) expansion to redundant systems with associated ground monitors. Additional modifications that are not necessary to meet the orbiter requirements, but that can enhance performance margin are (1) tightening of elevation antenna beam width from 1.3 deg to 0.5 deg and (2) split site configuration to provide azimuth and range coverage through rollout.

  2. Middle Holocene Unconformity in Seneca Lake, NY

    NASA Astrophysics Data System (ADS)

    Curtin, T. M.; Crocker, M.; Loddengaard, K.

    2008-12-01

    The post-glacial history of the Finger Lakes, NY have involved several changes in lake levels throughout the latest Pleistocene and Holocene, resulting from the changing position of the retreating Laurentide ice sheet, river outlet position, glacial rebound, and water balance. Previous studies of high-resolution seismic reflection profiles from three Finger Lakes define a middle Holocene erosional surface at water depths as great as 26 m in the northern end of each of lake. There are two proposed hypotheses to explain the origin of the observed erosional surfaces: 1) subaerial erosion during a lake lowstand and 2) erosion resulting from increased internal seiche activity. To evaluate these hypotheses, we examined a series of 2 to 5 m long piston cores collected along a north-south transect from one of the Finger Lakes, Seneca Lake. Cores were correlated using distinctive changes in the profiles of grain size, loss-on-ignition, and magnetic susceptibility. We recognize a significant erosional unconformity of early to middle Holocene sediment at modern depths <60 m because portions of the normal deepwater sediment sequence were missing in cores and were commonly replaced by a thin zone of sand and abraded shells (bivalves, gastropods). At water depths >60 m, the unconformity continues as a conformable zone. We attribute the unconformity to wave abrasion and nearshore current winnowing of the shoreface during a lowstand. With an assumption of an effective 20 m wave base, the depth to the low level lake surface responsible for the unconformity is estimated to be 40 m. The age of the unconformity is ~6 ka, based on radiocarbon ages of lithologic boundaries in the sediment cores. Because the unconformity grades into a conformable zone in deepwater cores that display no change in lithology, we hypothesize that the large-scale lake level drop is likely not the result of climate change, but rather a change in accommodation space in the northern portion of the lake basin due

  3. Telltale tumor infiltrating lymphocytes (TIL) in oral, head & neck cancer.

    PubMed

    Lei, Yu; Xie, Yuying; Tan, Yee Sun; Prince, Mark E; Moyer, Jeffrey S; Nör, Jacques; Wolf, Gregory T

    2016-10-01

    Evidence gleaned from recent studies on the role of tumor-infiltrating lymphocytes (TILs) suggests that cancer is not only a genetic disease but also an immunologic disease. Head and Neck Squamous Cell Carcinoma (HNSCC) has been a significant model to study cancer cell-immune cell interactions. First, immune cell infiltration is an important feature of these tumors. Second, HNSCC frequently develops resistance to immunogenic cytotoxicity, which provides a window to decipher how tumors engage the immune system to establish immune tolerance. Finally, chemoradiation therapy, as a central modality for HNSCC treatment, has been shown to elicit immune activation. The presence of effector immune cells in the tumor microenvironment is often associated with superior clinical response to adjuvant therapy. On the other hand, an activated immune system, in addition to limiting tumor initiation and progression, could also exert selective pressure to promote the growth of less immunogenic tumors, as a pivotal immunoediting process. But it remains unclear how cancer cell signaling regulates tumor immunogenicity and how to mitigate HNSCC-potentiated TIL suppression. In this review, we will revisit the prognostic role of TILs in HNSCC, and collectively discuss how cancer cell machinery impacts upon the plasticity of TILs.

  4. NY32649 ELECTRICAL SUBSTATION (BUILDING 2675) AND EDUCATION CENTER (BUILDING 2670) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    NY-326-49 ELECTRICAL SUBSTATION (BUILDING 2675) AND EDUCATION CENTER (BUILDING 2670) AT NORTH END OF ARIZONA AVENUE. VIEW TO NORTHEAST. - Plattsburgh Air Force Base, U.S. Route 9, Plattsburgh, Clinton County, NY

  5. 33 CFR 110.8 - Lake Champlain, N.Y. and Vt.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Lake Champlain, N.Y. and Vt. 110... ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.8 Lake Champlain, N.Y. and Vt. (a) Ticonderoga, N.Y. An area shoreward of a line bearing 312° from Ticonderoga Light to the southeast corner of...

  6. 78 FR 35596 - Special Local Regulation; Long Beach Regatta, Powerboat Race, Atlantic Ocean, Long Beach, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-13

    ... Race, Atlantic Ocean, Long Beach, NY AGENCY: Coast Guard, DHS. ACTION: Notice of Proposed Rulemaking... Atlantic Ocean off Long Beach, NY during the Long Beach Regatta Powerboat Race scheduled for August 24-25... powerboat racing regatta. The event will be held on the Atlantic Ocean off Long Beach, NY and will...

  7. 78 FR 7848 - New York Disaster Number NY-00130

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-04

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION New York Disaster Number NY-00130 AGENCY: U.S. Small Business Administration. ACTION: Amendment 5.... Small Business Administration, Processing and Disbursement Center, 14925 Kingsport Road, Fort Worth,...

  8. 78 FR 43063 - Drawbridge Operation Regulations; Arthur Kill, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-19

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Arthur Kill, NY AGENCY: Coast Guard... District, has issued a temporary deviation from the regulations governing the operation of the Arthur Kill AK Railroad Bridge across Arthur Kill, mile 11.6, between Staten Island, New York and Elizabeth,...

  9. 77 FR 16800 - Radio Broadcasting Services; Westfield, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-22

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Westfield, NY AGENCY: Federal Communications... filing procedures for comments, see 47 CFR 1.415 and 1.420. List of Subjects in 47 CFR Part 73 Radio, Radio broadcasting. Federal Communications Commission. Nazifa Sawez, Assistant Chief, Audio...

  10. 77 FR 50630 - Radio Broadcasting Services; Westfield, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-22

    ... COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Westfield, NY AGENCY: Federal Communications... Review Act, see 5 U.S.C. 801(a)(1)(A). List of Subjects in 47 CFR Part 73 Radio, Radio broadcasting...: PART 73--RADIO BROADCAST SERVICES 0 1. The authority citation for part 73 continues to read as...

  11. 78 FR 56609 - Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-13

    ... FR 34893) the draw of the Atlantic Beach Bridge at mile 0.4, across Reynolds Channel was required to... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY... Coast Guard is canceling the temporary deviation from the regulations published on June 11, 2013, (78...

  12. 78 FR 58458 - Drawbridge Operation Regulations; Quogue Canal, Southampton, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-24

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Quogue Canal, Southampton, NY AGENCY... the Quogue Bridge, mile 1.1, across Quogue Canal, at Southampton, New York. This temporary deviation... Quogue Bridge, mile 1.1, across Quogue Canal may keep one lift span in the closed position from October...

  13. Interoperability of Demand Response Resources Demonstration in NY

    SciTech Connect

    Wellington, Andre

    2014-03-31

    The Interoperability of Demand Response Resources Demonstration in NY (Interoperability Project) was awarded to Con Edison in 2009. The objective of the project was to develop and demonstrate methodologies to enhance the ability of customer sited Demand Response resources to integrate more effectively with electric delivery companies and regional transmission organizations.

  14. 78 FR 56610 - Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-13

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY... operation of the Atlantic Beach Bridge, mile 0.4, across Reynolds Channel, at Lawrence, New York. This... Atlantic Beach Bridge, across Reynolds Channel, mile 0.4, at Lawrence, New York, has a vertical...

  15. 77 FR 37316 - Drawbridge Operation Regulations; Reynolds Channel, Nassau, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-21

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Reynolds Channel, Nassau, NY AGENCY... the Long Beach Bridge, mile 4.7, across Reynolds Channel, at Nassau, New York. This temporary... Operations, telephone 202-366-9826. SUPPLEMENTARY INFORMATION: The Long Beach Bridge, across Reynolds...

  16. 78 FR 34893 - Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-11

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY... operation of the Atlantic Beach Bridge, mile 0.4, across Reynolds Channel, at Lawrence, New York. This...-9826. SUPPLEMENTARY INFORMATION: The Atlantic Beach Bridge, across Reynolds Channel, mile 0.4,...

  17. 78 FR 66265 - Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-05

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Reynolds Channel, Lawrence, NY... operation of the Atlantic Beach Bridge, mile 0.4, across Reynolds Channel, at Lawrence, New York. This... Atlantic Beach Bridge, across Reynolds Channel, mile 0.4, at Lawrence, New York, has a vertical...

  18. 78 FR 26508 - Drawbridge Operation Regulations; Reynolds Channel, Nassau, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-07

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Reynolds Channel, Nassau, NY AGENCY... the Long Beach Bridge, mile 4.7, across Reynolds Channel at Nassau, New York. Under this temporary... of 20 feet at mean high water, and 24 feet at mean low water in the closed position. The...

  19. 78 FR 37456 - Drawbridge Operation Regulations; Reynolds Channel, Nassau, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-21

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Reynolds Channel, Nassau, NY AGENCY... the Long Beach Bridge, mile 4.7, across Reynolds Channel at Nassau, New York. Under this temporary... Bridge has a vertical clearance of 20 feet at mean high water, and 24 feet at mean low water in...

  20. 78 FR 72003 - Amendment of Class E Airspace; Olean, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-02

    ... Cattaraugus County-Olean Airport, Olean, NY. (78 FR 52115). Interested parties were invited to participate in... Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a Regulatory Evaluation...: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p....

  1. 76 FR 61130 - New York Disaster Number NY-00110

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-03

    ... ADMINISTRATION New York Disaster Number NY-00110 AGENCY: U.S. Small Business Administration. ACTION: Amendment 3. SUMMARY: This is an amendment of the Presidential declaration of a major disaster for the State of New... INFORMATION: The notice of the President's major disaster declaration for the State of New York, dated...

  2. 76 FR 68804 - New York Disaster Number NY-00113

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-07

    ... ADMINISTRATION New York Disaster Number NY-00113 AGENCY: U.S. Small Business Administration. ACTION: Amendment 6... Only for the State of New York (FEMA-4031-DR), dated 09/23/2011. Incident: Remnants of Tropical Storm... Private Non-Profit organizations in the State of New York, dated 09/23/2011, is hereby amended to...

  3. 76 FR 68803 - New York Disaster Number NY-00108

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-07

    ... ADMINISTRATION New York Disaster Number NY-00108 AGENCY: U.S. Small Business Administration. ACTION: Amendment 8. SUMMARY: This is an amendment of the Presidential declaration of a major disaster for the State of New... the President's major disaster declaration for the State of New York, dated 08/31/2011 is...

  4. 76 FR 59176 - New York Disaster Number NY-00110

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-23

    ... ADMINISTRATION New York Disaster Number NY-00110 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... INFORMATION: The notice of the Presidential disaster declaration for the State of New York, dated 09/13/2011... Loans Only): New York: Albany, Saratoga. All other information in the original declaration...

  5. 76 FR 66111 - New York Disaster Number NY-00113

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ... ADMINISTRATION New York Disaster Number NY-00113 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4... Only for the State of New York (FEMA-4031-DR), dated 09/23/2011. Incident: Remnants of Tropical Storm... Private Non-Profit organizations in the State of New York, dated 09/23/2011, is hereby amended to...

  6. 76 FR 59766 - New York Disaster Number NY-00110

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-27

    ... ADMINISTRATION New York Disaster Number NY-00110 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2. SUMMARY: This is an amendment of the Presidential declaration of a major disaster for the State of New...: The notice of the Presidential disaster declaration for the State of New York, dated 09/13/2011...

  7. 76 FR 11553 - New York Disaster #NY-00102

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-02

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION New York Disaster NY-00102 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of New York (FEMA- 1957-DR), dated 02/18/2011. Incident: Severe Winter Storm and...

  8. 76 FR 40767 - New York Disaster Number NY-00105

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... ADMINISTRATION New York Disaster Number NY-00105 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Only for the State of New York (FEMA-1993-DR), dated 06/10/2011 . Incident: Severe Storms, Flooding... major disaster declaration for Private Non-Profit organizations in the State of New York, dated...

  9. 76 FR 59178 - New York Disaster Number NY-00109

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-23

    ... ADMINISTRATION New York Disaster Number NY-00109 AGENCY: U.S. Small Business Administration. ACTION: Amendment 7... Only for the State of New York (FEMA-4020-DR), dated 08/31/2011. Incident: Hurricane Irene. Incident... Non-Profit organizations in the State of New York, dated 08/31/2011, is hereby amended to include...

  10. 78 FR 48537 - New York Disaster # NY-00135

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-08

    ... ADMINISTRATION New York Disaster NY-00135 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of New York dated 08/02... Counties: New York: Chenango, Cortland, Fulton, Hamilton, Lewis, Onondaga, Oswego, Otsego, Saint...

  11. 75 FR 41558 - New York Disaster Number NY-00089

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    ... ADMINISTRATION New York Disaster Number NY-00089 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... Only for the State of New York (FEMA-1899-DR), dated 04/16/2010. Incident: Severe Storms and Flooding... Private Non-Profit organizations in the State of New York, dated 04/16/2010, is hereby amended to...

  12. 76 FR 55721 - New York Disaster #NY-00109

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-08

    ... ADMINISTRATION New York Disaster NY-00109 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of New York (FEMA- 4020-DR), dated 08/31/2011. Incident: Hurricane Irene. Incident Period: 08/26..., Clinton, Delaware, Dutchess, Essex, Greene, Montgomery, Nassau, New York, Queens, Rensselaer,...

  13. 78 FR 44187 - New York Disaster # NY-00136

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-23

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION New York Disaster NY-00136 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of New York (FEMA- 4129-DR), dated 07/12/2013. Incident: Severe Storms and Flooding....

  14. 76 FR 55721 - New York Disaster #NY-00108

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-08

    ... ADMINISTRATION New York Disaster NY-00108 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for the State of New York (FEMA.... Contiguous Counties (Economic Injury Loans Only): New York: Broome, Chenango, Clinton, Columbia,...

  15. 76 FR 67245 - New York Disaster Number NY-00110

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ... ADMINISTRATION New York Disaster Number NY-00110 AGENCY: U.S. Small Business Administration. ACTION: Amendment 6... INFORMATION: The notice of the Presidential disaster declaration for the State of New York, dated 09/13/2011... Injury Loans Only): New York: Saint Lawrence. All other information in the original declaration...

  16. 76 FR 64420 - New York Disaster Number NY-00113

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ... ADMINISTRATION New York Disaster Number NY-00113 AGENCY: U.S. Small Business Administration. ACTION: Amendment 3... Only for the State of New York (FEMA-4031-DR). dated 09/23/2011. Incident: Remnants of Tropical Storm... Private Non-Profit organizations in the State of New York, dated 09/23/2011, is hereby amended to...

  17. 76 FR 64420 - NEW YORK Disaster Number NY-00110

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ... ADMINISTRATION NEW YORK Disaster Number NY-00110 AGENCY: U.S. Small Business Administration. ACTION: Amendment 5... INFORMATION: The notice of the Presidential disaster declaration for the State of NEW YORK, dated 09/13/2011... Only) New York, Hamilton. All other information in the original declaration remains unchanged....

  18. 76 FR 58329 - New York Disaster Number NY-00108

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-20

    ... ADMINISTRATION New York Disaster Number NY-00108 AGENCY: U.S. Small Business Administration. ACTION: Amendment 5. SUMMARY: This is an amendment of the Presidential declaration of a major disaster for the State of New... the Presidential disaster declaration for the State of NEW YORK, dated 08/31/2011, is hereby...

  19. 76 FR 18289 - New York Disaster Number NY-00102

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... ADMINISTRATION New York Disaster Number NY-00102 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... Only for the State of New York (FEMA-1957-DR), dated 02/18/2011. Incident: Severe winter storm and... Private Non-Profit organizations in the State of New York, dated 02/18/2011, is hereby amended to...

  20. 76 FR 62131 - New York Disaster Number NY-00113

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-06

    ... ADMINISTRATION New York Disaster Number NY-00113 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... only for the State of New York (FEMA-4031-DR), dated 09/23/2011. Incident: Remnants of Tropical Storm... Private Non-Profit organizations in the State of New York, dated 09/23/2011, is hereby amended to...

  1. 76 FR 59178 - New York Disaster Number NY-00108

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-23

    ... ADMINISTRATION New York Disaster Number NY-00108 AGENCY: U.S. Small Business Administration. ACTION: Amendment 7... the Presidential disaster declaration for the State of New York, dated 08/31/2011 is hereby amended to... and Economic Injury Loans): Herkimer. Contiguous Counties: (Economic Injury Loans Only): New...

  2. 78 FR 47816 - New York Disaster Number NY-00136

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-06

    ... ADMINISTRATION New York Disaster Number NY-00136 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... Only for the State of New York (FEMA-4129-DR), dated 07/12/2013. Incident: Severe Storms and Flooding... Private Non-Profit organizations in the State of NEW YORK, dated 07/12/2013, is hereby amended to...

  3. 76 FR 67245 - New York Disaster Number NY-00113

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-31

    ... ADMINISTRATION New York Disaster Number NY-00113 AGENCY: U.S. Small Business Administration. ACTION: Amendment 5... Only for the State of New York (FEMA-4031-DR), dated 09/23/2011. Incident: Remnants of Tropical Storm... Private Non-Profit organizations in the State of New York, dated 09/23/2011, is hereby amended to...

  4. 77 FR 69915 - New York Disaster Number NY-00130

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-21

    ... ADMINISTRATION New York Disaster Number NY-00130 AGENCY: U.S. Small Business Administration. ACTION: Amendment 2... disaster declaration for the State of NEW YORK, dated 10/30/2012 is hereby amended to include the following... Loans): Orange, Putnam, Sullivan, Ulster. Contiguous Counties: (Economic Injury Loans Only): New...

  5. 78 FR 15109 - New York Disaster Number NY-00130

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-08

    ... ADMINISTRATION New York Disaster Number NY-00130 AGENCY: U.S. Small Business Administration. ACTION: Amendment 6. SUMMARY: This is an amendment of the Presidential declaration of a major disaster for the State of New... the President's major disaster declaration for the State of New York, dated 10/30/2012 is...

  6. 76 FR 68804 - New York Disaster Number NY-00110

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-07

    ... ADMINISTRATION New York Disaster Number NY-00110 AGENCY: U.S. Small Business Administration. ACTION: Amendment 7. SUMMARY: This is an amendment of the Presidential declaration of a major disaster for the State of New... INFORMATION: The notice of the President's major disaster declaration for the State of New York, dated...

  7. 76 FR 61775 - New York Disaster #NY-00113

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION New York Disaster NY-00113 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... State of New York (FEMA- 4031-DR), dated 09/23/2011. Incident: Remnants of Tropical Storm Lee....

  8. 77 FR 74907 - New York Disaster Number NY-00130

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-18

    ... ADMINISTRATION New York Disaster Number NY-00130 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4. SUMMARY: This is an amendment of the Presidential declaration of a major disaster for the State of New... the President's major disaster declaration for the State of New York, dated 10/30/2012 is...

  9. 76 FR 59177 - New York Disaster #NY-00110

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-23

    ... ADMINISTRATION New York Disaster NY-00110 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for the State of New York (FEMA... Injury Loans Only): New York: Chemung, Cortland, Greene, Herkimer, Madison, Montgomery, Oneida,...

  10. 77 FR 68195 - New York Disaster Number NY-00130

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-15

    ... ADMINISTRATION New York Disaster Number NY-00130 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... disaster declaration for the State of NEW YORK, dated 10/30/2012 is hereby amended to include the following.... New Jersey: Passaic. New York: Orange, Putnam. All other information in the original...

  11. 77 FR 64372 - New York Disaster #NY-00126

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-19

    ... ADMINISTRATION New York Disaster NY-00126 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of New York dated 10/10... adversely affected by the disaster: Primary Counties: Sullivan. Contiguous Counties: New York:...

  12. 76 FR 58328 - New York Disaster Number NY-00108

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-20

    ... ADMINISTRATION New York Disaster Number NY-00108 AGENCY: U.S. Small Business Administration. ACTION: Amendment 6... the Presidential disaster declaration for the State of New York, dated 08/31/2011 is hereby amended to...): New York: New York. All other information in the original declaration remains unchanged. (Catalog...

  13. 77 FR 76587 - New York Disaster Number NY-00131

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-28

    ... ADMINISTRATION New York Disaster Number NY-00131 AGENCY: U.S. Small Business Administration. ACTION: Amendment 3... Only for the State of New York (FEMA--4085--DR), dated 11/03/2012. Incident: Hurricane Sandy. Incident... Non-Profit organizations in the State of New York, dated 11/03/2012, is hereby amended to include...

  14. 78 FR 47817 - New York Disaster Number NY-00136

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-06

    ... ADMINISTRATION New York Disaster Number NY-00136 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Only for the State of New York (FEMA--4129--DR), dated 07/12/2013. Incident: Severe Storms and Flooding... Private Non-Profit organizations in the State of New York, dated 07/12/2013, is hereby amended to...

  15. 75 FR 41558 - New York Disaster Number NY-00089

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-16

    ... ADMINISTRATION New York Disaster Number NY-00089 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1... Only for the State of New York (FEMA-1899-DR), dated 04/16/2010. Incident: Severe Storms and Flooding... Private Non-Profit organizations in the State of New York, dated 04/16/2010, is hereby amended to...

  16. Rapid variability of the EXor star NY Ori

    NASA Astrophysics Data System (ADS)

    Lorenzetti, D.; Arkharov, A. A.; Efimova, N.; Giannini, T.; Antoniucci, S.; Di Paola, A.; Larionov, V. M.

    2016-03-01

    We report on a rapid brightness variability of the classical EXor star NY Ori observed with the AZT24 1m IR telescope (Campo Imperatore, Italy), as a part of our program EXORCISM (EXOR OptiCal and Infrared Systematic Monitoring - Antoniucci et al. 2013 PPVI; Lorenzetti et al. 2009 ApJ 693, 1056).

  17. No Retreat: Lorna Peterson--University at Buffalo, NY

    ERIC Educational Resources Information Center

    Library Journal, 2005

    2005-01-01

    When Clark Atlanta University announced in 2003 it would close its library school, Lorna Peterson, a library educator at the University at Buffalo, NY, mounted a campaign to save the program, which has graduated more black library leaders than any other. "I did not want this to happen without a fight. It cannot be said that librarians, Friends of…

  18. 76 FR 35937 - New York Disaster #NY-00105

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-20

    ... From the Federal Register Online via the Government Publishing Office U.S. SMALL BUSINESS ADMINISTRATION New York Disaster NY-00105 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY.../12/2012. ADDRESSES: Submit completed loan applications to: U.S. Small Business...

  19. 33 CFR 110.84b - Buffalo, N.Y.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... REGULATIONS Special Anchorage Areas § 110.84b Buffalo, N.Y. The area within the Port of Buffalo known as Port of Buffalo Small Boat Harbor commencing at a point on shore at latitude 42°51′05″ N., longitude...

  20. Results of the NY-Alesund ozone measurements intercomparison NAOMI

    NASA Technical Reports Server (NTRS)

    Steinbrecht, W.; Gross, M.; McGee, T.; Neuber, R.; Gathen, P. V. D.; Wahl, P.; Klein, U.; Langer, J.

    1998-01-01

    Worldwide, about ten Differential Absorption Lidars are used for long-term monitoring of stratospheric ozone. These systems are an important component of the Network for the Detection of Stratospheric Change. Although DIALs are self-calibrating in principle, regular intercomparisons with other ozone-lidars, microwave radiometers or ozone-sondes are highly desirable to ensure high data quality at a well known level. The Network for the Detection of Stratospheric Change (NDSC) validation policy suggests that such intercomparisons be "blind", meaning all participants submit their data to an impartial referee, without seeing results from the other participants. Here we report on the "blind" intercomparison taking place from January 20th to February 10th 1998 at Ny-Alesund, Spitsbergen (78.92 deg N, 11.95 deg E). Participating groups were from the Alfred Wegener Institute, Potsdam, operating the NDSC DIAL system at Ny-Alesund, from the University of Bremen operating the NDSC microwave radiometer for ozone profiling at Ny-Alesund, and the NASA Goddard Space Flight Center group with the "NDSC travelling standard" STROZ-LITE. The first author acted as the impartial referee. Also used for the intercomparison were data from ECC-6A/Vaisala RS80 ozone sondes routinely launched at Ny-Alesund by the AWI group. A 1% KI solution (3 ml) and the 1986 ECC pump correction (1.092 at 5 hPa) are used. The ECC-data were available to all participants during the campaign and thus were not "blind". Table 1 summarizes the expected performance of the instruments participating in the ozone intercomparison reported in this paper.

  1. Vaccination with NY-ESO-1 overlapping peptides mixed with Picibanil OK-432 and montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    PubMed

    Wada, Hisashi; Isobe, Midori; Kakimi, Kazuhiro; Mizote, Yu; Eikawa, Shingo; Sato, Eiichi; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Yamasaki, Makoto; Miyata, Hiroshi; Matsushita, Hirokazu; Udono, Heiichiro; Seto, Yasuyuki; Yamada, Kazuhiro; Nishikawa, Hiroyoshi; Pan, Linda; Venhaus, Ralph; Oka, Mikio; Doki, Yuichiro; Nakayama, Eiichi

    2014-01-01

    We conducted a clinical trial of an NY-ESO-1 cancer vaccine using 4 synthetic overlapping long peptides (OLP; peptides #1, 79-108; #2, 100-129; #3, 121-150; and #4, 142-173) that include a highly immunogenic region of the NY-ESO-1 molecule. Nine patients were immunized with 0.25 mg each of three 30-mer and a 32-mer long NY-ESO-1 OLP mixed with 0.2 KE Picibanil OK-432 and 1.25 mL Montanide ISA-51. The primary endpoints of this study were safety and NY-ESO-1 immune responses. Five to 18 injections of the NY-ESO-1 OLP vaccine were well tolerated. Vaccine-related adverse events observed were fever and injection site reaction (grade 1 and 2). Two patients showed stable disease after vaccination. An NY-ESO-1-specific humoral immune response was observed in all patients and an antibody against peptide #3 (121-150) was detected firstly and strongly after vaccination. NY-ESO-1 CD4 and CD8 T-cell responses were elicited in these patients and their epitopes were identified. Using a multifunctional cytokine assay, the number of single or double cytokine-producing cells was increased in NY-ESO-1-specific CD4 and CD8 T cells after vaccination. Multiple cytokine-producing cells were observed in PD-1 (-) and PD-1 (+) CD4 T cells. In conclusion, our study indicated that the NY-ESO-1 OLP vaccine mixed with Picibanil OK-432 and Montanide ISA-51 was well tolerated and elicited NY-ESO-1-specific humoral and CD4 and CD8 T-cell responses in immunized patients.

  2. Tumor infiltrating lymphocytes (TILs) before and after neoadjuvant chemoradiotherapy and its clinical utility for rectal cancer

    PubMed Central

    Teng, Feifei; Mu, Dianbin; Meng, Xiangjiao; Kong, Li; Zhu, Hui; Liu, Sujing; Zhang, Jianbo; Yu, Jinming

    2015-01-01

    Backgrounds: Radiotherapy (RT) and chemotherapy (CT) can potentiate systemic antitumor immune effect. However, immunomodulation during RT or CT and their clinical implications in rectal cancer have not been thoroughly investigated. Methods: We investigated alterations in the densities of tumor infiltrating lymphocytes (TILs) during chemoradiation and their clinical utilities in patients with rectal cancer. We analyzed 136 rectal cancer patients who underwent neoadjuvant RT, CT or chemoradiotherapy (CRT), followed by radical resection retrospectively. Pretreatment biopsy specimens and posttreatment resected specimens of all patients were immunostained for CD3 and CD8. The predictive value of TILs to neoadjuvant treatment and prognosis were examined. Results: Densities of CD3+ and CD8+TILs in posttreatment specimens after RT, CT or CRT were all significantly higher than those in pretreatment specimens. There were no significant differences between each two of these three groups. High pretreatment CD3+ and CD8+TILs were associated with good response (TRG ≥ 3) after neoadjuvant treatments (P = 0.033 and 0.021). High CD3+TILs and CD8+TILs in pretreatment biopsy specimens were significantly associated with favorable disease free survival (DFS) (P = 0.010 and P = 0.022) and overall survival (OS) (P = 0.019 and P = 0.003). Conclusions: We may, thus, conclude that chemoradiation can enhance local immune response by increased TILs. High TILs densities before treatment are associated with good response to neoadjuvant chemoradiotherapy and a favorable prognosis. PMID:26269765

  3. Increased NY-ESO-1 expression and reduced infiltrating CD3+ T cells in cutaneous melanoma.

    PubMed

    Giavina-Bianchi, Mara; Giavina-Bianchi, Pedro; Sotto, Mirian Nacagami; Muzikansky, Alona; Kalil, Jorge; Festa-Neto, Cyro; Duncan, Lyn M

    2015-01-01

    NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P = 0.007) and inversely correlated with superficial spreading melanoma (P < 0.02). NY-ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P = 0.017). When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P = 0.010) or as isolated cells (P = 0.002) than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.

  4. NY-ESO-1 protein glycosylated by yeast induces enhanced immune responses.

    PubMed

    Wadle, Andreas; Mischo, Axel; Strahl, Sabine; Nishikawa, Hiroyoshi; Held, Gerhard; Neumann, Frank; Wullner, Beate; Fischer, Eliane; Kleber, Sascha; Karbach, Julia; Jager, Elke; Shiku, Hiroshi; Odunsi, Kunle; Shrikant, Protul A; Knuth, Alexander; Cerundolo, Vincenzo; Renner, Christoph

    2010-11-01

    Vaccine strategies that target dendritic cells to elicit potent cellular immunity are the subject of intense research. Here we report that the genetically engineered yeast Saccharomyces cerevisiae, expressing the full-length tumour-associated antigen NY-ESO-1, is a versatile host for protein production. Exposing dendritic cells (DCs) to soluble NY-ESO-1 protein linked to the yeast a-agglutinin 2 protein (Aga2p) protein resulted in protein uptake, processing and MHC class I cross-presentation of NY-ESO-1-derived peptides. The process of antigen uptake and cross-presentation was dependent on the glycosylation pattern of NY-ESO-1-Aga2p protein and the presence of accessible mannose receptors. In addition, NY-ESO-1-Aga2p protein uptake by dendritic cells resulted in recognition by HLA-DP4 NY-ESO-1-specific CD4(+) T cells, indicating MHC class II presentation. Finally, vaccination of mice with yeast-derived NY-ESO-1-Aga2p protein led to an enhanced humoral and cellular immune response, when compared to the bacterially expressed NY-ESO-1 protein. Together, these data demonstrate that yeast-derived full-length NY-ESO-1-Aga2p protein is processed and presented efficiently by MHC class I and II complexes and warrants clinical trials to determine the potential value of S. cerevisiae as a host for cancer vaccine development.

  5. NY-ESO-1 expression in synovial sarcoma and other mesenchymal tumors: significance for NY-ESO-1-based targeted therapy and differential diagnosis.

    PubMed

    Lai, Jin-Ping; Robbins, Paul F; Raffeld, Mark; Aung, Phyu Phyu; Tsokos, Maria; Rosenberg, Steven A; Miettinen, Markku M; Lee, Chyi-Chia Richard

    2012-06-01

    A promising targeted therapy against NY-ESO-1 (CTAG 1B) using genetically modified T-cells in synovial sarcomas was recently demonstrated in a clinical trial at the NCI. To investigate the role of NY-ESO-1 immunohistochemistry in patient selection and gain better insight into the incidence of NY-ESO-1 expression in synovial sarcomas and other mesenchymal tumors, we evaluated NY-ESO-1 expression by immunohistochemistry in 417 tumors. This collection of samples included: 50 SS18/SSX1/2 fusion positive synovial sarcomas, 155 gastrointestinal stromal tumors (GIST), 135 other spindle cell sarcomas as well as 77 other sarcomas (chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, rhabdomyosarcoma, angiosarcoma, malignant mesothelioma, and Ewing's sarcoma). We report that 76% of synovial sarcomas expressed NY-ESO-1 in a strong and diffuse pattern (2-3+, >50-70% of tumor cells). In contrast, only rare cases of other spindle cell mesenchymal tumor expressed NY-ESO-1 (GIST (2/155), malignant peripheral nerve sheath tumors (1/34), and dermatofibrosarcoma protuberans (2/20)). Individual cases of other sarcomas (angiosarcoma, malignant mesothelioma, chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, and Ewing's sarcoma) were positive for NY-ESO-1. However, no positive cases were identified amongst our cohort of leiomyosarcomas (0/24), hemangiopericytoma/solitary fibrous tumors (0/40), and cellular schwannomas (0/17). In summary, we find that NY-ESO-1 is strongly and diffusely expressed in a majority of synovial sarcomas, but only rarely in other mesenchymal lesions. Beyond its role in patient selection for targeted therapy, immunohistochemistry for NY-ESO-1 may be diagnostically useful for the distinction of synovial sarcoma from other spindle cell neoplasms.

  6. 33 CFR 110.87 - Henderson Harbor, N.Y.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.87 Henderson Harbor, N.Y. (a) Area A. The area in the... latitude 43°51′08.8″ N, longitude 76°12′08.9″ W, thence to latitude 43°51′09.0″ N, longitude 76°12′19.0″ W, thence to latitude 43°51′33.4″ N, longitude 76°12′19.0″ W, thence to latitude 43°51′33.4″ N, longitude...

  7. Adoptive TIL Transfer in the Adjuvant Setting for Melanoma: Long-Term Patient Survival

    PubMed Central

    Khammari, Amir; Knol, Anne-Chantal; Nguyen, Jean-Michel; Bossard, Céline; Denis, Marc-Guillaume; Pandolfino, Marie-Christine; Quéreux, Gaëlle; Bercegeay, Sylvain; Dréno, Brigitte

    2014-01-01

    Two first analyses of our clinical trial on TIL as adjuvant therapy for melanoma were published in 2002 and 2007. We present here an update of the clinical results after a 17-year median followup. In this trial, disease-free patients were randomly assigned to receive either TIL/IL-2 or IL-2. The relapse-free survival (RFS) was the primary objective. Eighty-eight patients were enrolled. A new analysis performed in May 2013 did not show significant changes in RFS or OS duration. However, our first finding on the association between the number of invaded lymph nodes and TIL effectiveness was strengthened. The Cox model adjusted on this interaction showed for the first time a significant treatment effect when considering the overall population, both on the RFS and OS. Patients treated with TIL had a longer RFS (P = 0.023) or OS (P = 0.020). This study being with a very long followup (17 years), confirmed the association between TIL effectiveness and the number of invaded lymph nodes, indicating that a low tumor burden could be a crucial factor enhancing the curative effect of TIL in possible microscopic residual disease. Moreover, we confirmed that a prolonged survival was associated with the presence of specific TIL and a decrease in Foxp3 expression. PMID:24741578

  8. Flow and chloride transport in the tidal Hudson River, NY

    USGS Publications Warehouse

    Weiss, Lawrence A.; Schaffranek, Raymond W.; de Vries, M. Peter

    1994-01-01

    A one-dimensional dynamic-flow model and a one-dimensional solute-transport model were used to evaluate the effects of hypothetical public-supply water withdrawals on saltwater intrusion in a 133-mile reach of the tidal Hudson River between Green Island dam, near Troy, N.Y., and Hastings-on-Hudson, N.Y. Regression techniques were used in analyses of current and extreme historical conditions, and numerical models were used to investigate the effect of various water withdrawals. Of four withdrawal scenarios investigated, simulations of a 27-day period during which discharges at Green Island dam averaged 7,090 ft3/s indicate that increasing the present Chelsea pumping-station withdrawal rate of 100 Mgal/d (million gallons per day) to 300 Mgal/d would have the least effect on upstream saltwater movement. A 90-day simulation, during which discharges at Green Island dam averaged 25,200 ft3/s, indicates that withdrawals of 1,940 Mgal/d at Chelsea would not measurably increase chloride concentrations at Chelsea under normal tidal and meteorological conditions, but withdrawals of twice that rate (3,880 Mgal/d) could increase the chloride concentration at Chelsea to 250 mg/L.

  9. Cancer/testis antigen NY-SAR-35 enhances cell proliferation, migration, and invasion.

    PubMed

    Song, Myung-Ha; Kim, Ye-Rin; Lee, Jun-Won; Lee, Chang-Hun; Lee, Sang-Yull

    2016-02-01

    The cancer/testis antigen NY-SAR-35 is aberrantly expressed in various cancer tissues and cancer cell lines but not in normal tissues except for the testis. A previous study demonstrated that the expression of NY-SAR-35 is activated by hypomethylation in cancer cells. However, the functions of this antigen remain unexplored. In the present study, we investigated the role of NY-SAR‑35 in human embryonic kidney (HEK) 293 cells using exogenous expression system of the gene. NY-SAR‑35 was predominantly expressed at the cytoplasm and was mainly observed in spermatogonia and spermatocytes. Expression of NY-SAR-35 in stable HEK293 transfectant clones was 2-fold higher than the control cells promoting cell growth and proliferation. NY-SAR-35 overexpression also enhanced cell migration and invasion ~2-fold and 4-fold more than the control, respectively. In contrast, small interfering RNA-mediated knockdown of NY-SAR-35 suppressed cell proliferation, migration, and invasion in HEK293 stable transfectants. We concluded that NY-SAR-35 as a cancer/testis antigen enhanced cell proliferation and invasion.

  10. 33 CFR 165.166 - Safety Zone: Macy's July 4th Fireworks, East River, NY.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CFR 165.23 apply. (2) No vessels, except the Staten Island Ferries, will be allowed to transit the... Fireworks, East River, NY. 165.166 Section 165.166 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.166 Safety Zone: Macy's July 4th Fireworks, East River, NY. (a) Regulated area. The following...

  11. 33 CFR 165.166 - Safety Zone: Macy's July 4th Fireworks, East River, NY.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... CFR 165.23 apply. (2) No vessels, except the Staten Island Ferries, will be allowed to transit the... Fireworks, East River, NY. 165.166 Section 165.166 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.166 Safety Zone: Macy's July 4th Fireworks, East River, NY. (a) Regulated area. The following...

  12. 33 CFR 165.166 - Safety Zone: Macy's July 4th Fireworks, East River, NY.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... CFR 165.23 apply. (2) No vessels, except the Staten Island Ferries, will be allowed to transit the... Fireworks, East River, NY. 165.166 Section 165.166 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.166 Safety Zone: Macy's July 4th Fireworks, East River, NY. (a) Regulated area. The following...

  13. 33 CFR 165.166 - Safety Zone: Macy's July 4th Fireworks, East River, NY.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... CFR 165.23 apply. (2) No vessels, except the Staten Island Ferries, will be allowed to transit the... Fireworks, East River, NY. 165.166 Section 165.166 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.166 Safety Zone: Macy's July 4th Fireworks, East River, NY. (a) Regulated area. The following...

  14. 33 CFR 165.166 - Safety Zone: Macy's July 4th Fireworks, East River, NY.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... CFR 165.23 apply. (2) No vessels, except the Staten Island Ferries, will be allowed to transit the... Fireworks, East River, NY. 165.166 Section 165.166 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.166 Safety Zone: Macy's July 4th Fireworks, East River, NY. (a) Regulated area. The following...

  15. 75 FR 38710 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-06

    ... Championships, Huntington Bay, NY'' in the Federal Register (75 FR 13454). The Coast Guard received no comments...-2009-0520) in the Federal Register (74 FR 51243). The notice proposed a regulated area encompassing 100... Bay, NY in the Federal Register (75 FR 13454). This notice proposes a 100 yard regulated area...

  16. 33 CFR 207.60 - Federal Dam, Hudson River, Troy, N.Y.; pool level.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Federal Dam, Hudson River, Troy, N.Y.; pool level. 207.60 Section 207.60 Navigation and Navigable Waters CORPS OF ENGINEERS..., N.Y.; pool level. (a) Whenever the elevation of the pool created by the Federal dam at Troy,...

  17. 33 CFR 207.580 - Buffalo Harbor, N.Y.; use, administration, and navigation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Buffalo Harbor, N.Y.; use, administration, and navigation. 207.580 Section 207.580 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.580 Buffalo Harbor, N.Y.;...

  18. 33 CFR 207.600 - Rochester (Charlotte) Harbor, N.Y.; use, administration, and navigation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Rochester (Charlotte) Harbor, N.Y.; use, administration, and navigation. 207.600 Section 207.600 Navigation and Navigable Waters CORPS OF... (Charlotte) Harbor, N.Y.; use, administration, and navigation. (a)-(b) (c) No vessel shall moor or anchor...

  19. 33 CFR 110.85 - Niagara River, Youngstown, N.Y.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Niagara River, Youngstown, N.Y. 110.85 Section 110.85 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.85 Niagara River, Youngstown, N.Y. (a) Area...

  20. 33 CFR 110.150 - Block Island Sound, N.Y.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Block Island Sound, N.Y. 110.150 Section 110.150 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.150 Block Island Sound, N.Y. (a) The anchorage ground. A...

  1. 33 CFR 207.50 - Hudson River Lock at Troy, N.Y.; navigation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Hudson River Lock at Troy, N.Y.; navigation. 207.50 Section 207.50 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.50 Hudson River Lock at Troy, N.Y.; navigation....

  2. 33 CFR 162.15 - Manhasset Bay, N.Y.; seaplane restricted area.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Manhasset Bay, N.Y.; seaplane restricted area. 162.15 Section 162.15 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... Bay, N.Y.; seaplane restricted area. (a) The restricted area. An area in Manhasset Bay between...

  3. 33 CFR 165.172 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Brooklyn tower. (b) Regulations. (1) The general regulations contained in 33 CFR 165.23 apply. (2) Entering..., Gravesend Bay, Brooklyn, NY. 165.172 Section 165.172 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.172 Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY. (a) Location. The following...

  4. 33 CFR 165.172 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Brooklyn tower. (b) Regulations. (1) The general regulations contained in 33 CFR 165.23 apply. (2) Entering..., Gravesend Bay, Brooklyn, NY. 165.172 Section 165.172 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.172 Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY. (a) Location. The following...

  5. 78 FR 25008 - Safety Zone; Fairfield Estates Fireworks Display, Atlantic Ocean, Sagaponack, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-29

    ..., Atlantic Ocean, Sagaponack, NY AGENCY: Coast Guard, DHS. ACTION: Notice of Proposed Rulemaking. SUMMARY... of the Atlantic Ocean within a 1000 foot radius of the fireworks barge located 1000 feet south of the... Zone; Fairfield Estates Fireworks Display, Atlantic Ocean, Sagaponack, NY. (a) Location. The...

  6. 78 FR 41844 - Safety Zone; Fairfield Estates Fireworks Display, Atlantic Ocean, Sagaponack, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-12

    ..., Atlantic Ocean, Sagaponack, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the navigable waters of the Atlantic Ocean, in... Zone; Fairfield Estates Fireworks Display, Atlantic Ocean, Sagaponack, NY in the Federal Register...

  7. 33 CFR 110.150 - Block Island Sound, N.Y.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Block Island Sound, N.Y. 110.150... ANCHORAGE REGULATIONS Anchorage Grounds § 110.150 Block Island Sound, N.Y. (a) The anchorage ground. A 3/4- by 2-mile rectangular area approximately 3 miles east-northeast of Gardiners Island with...

  8. 77 FR 51473 - Safety Zone; Bostock 50th Anniversary Fireworks, Long Island Sound; Manursing Island, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-24

    ... Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking COTP..., NY in the Federal Register (77 FR 34894). We received no comments on the proposed rule. No public... Island Sound; Manursing Island, NY AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The Coast...

  9. 78 FR 56607 - Drawbridge Operation Regulation; Hudson River, Troy and Green Island, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-13

    ... Regulations; Hudson River, Troy and Green Island, NY,'' in the Federal Register (78 FR 31454). We received no... Green Island, NY AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The Coast Guard amends the..., mile 152.7, between Troy and Green Island, New York. The owner of the bridge, New York State...

  10. 33 CFR 110.150 - Block Island Sound, N.Y.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Block Island Sound, N.Y. 110.150... ANCHORAGE REGULATIONS Anchorage Grounds § 110.150 Block Island Sound, N.Y. (a) The anchorage ground. A 3/4- by 2-mile rectangular area approximately 3 miles east-northeast of Gardiners Island with...

  11. 33 CFR 110.150 - Block Island Sound, N.Y.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Block Island Sound, N.Y. 110.150... ANCHORAGE REGULATIONS Anchorage Grounds § 110.150 Block Island Sound, N.Y. (a) The anchorage ground. A 3/4- by 2-mile rectangular area approximately 3 miles east-northeast of Gardiners Island with...

  12. 33 CFR 110.150 - Block Island Sound, N.Y.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Block Island Sound, N.Y. 110.150... ANCHORAGE REGULATIONS Anchorage Grounds § 110.150 Block Island Sound, N.Y. (a) The anchorage ground. A 3/4- by 2-mile rectangular area approximately 3 miles east-northeast of Gardiners Island with...

  13. 77 FR 39408 - Safety Zone; Buffalo July 4th Fireworks, Lake Erie, Buffalo, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-03

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Buffalo July 4th Fireworks, Lake Erie, Buffalo, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on Lake Erie, Buffalo, NY. This safety zone is intended to...

  14. 33 CFR 165.172 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., Gravesend Bay, Brooklyn, NY. 165.172 Section 165.172 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.172 Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY. (a) Location. The following area is a safety zone: All navigable waters of Gravesend Bay within a 110-yard radius of a point...

  15. 33 CFR 165.172 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., Gravesend Bay, Brooklyn, NY. 165.172 Section 165.172 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.172 Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY. (a) Location. The following area is a safety zone: All navigable waters of Gravesend Bay within a 110-yard radius of a point...

  16. 33 CFR 165.165 - Regulated Navigation Area; Hudson River South of the Troy Locks, NY.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... area that has operating restrictions in effect must contact the COTP at telephone number (718) 354-4356... River South of the Troy Locks, NY. 165.165 Section 165.165 Navigation and Navigable Waters COAST GUARD... § 165.165 Regulated Navigation Area; Hudson River South of the Troy Locks, NY. (a) Regulated...

  17. 75 FR 25289 - Notice of Intent To Repatriate Cultural Items: Rochester Museum & Science Center, Rochester, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ... acquired two large wooden medicine faces from Alvin Dewey, Rochester, NY (AE 2870/D 8363/29.259.17 and AE... Mr. & Mrs. Warner Palmer, Albion, NY (AE 10315/66.222.1 and AE 10316/66.222.2). Both faces were...

  18. Epigenetic potentiation of NY-ESO-1 vaccine therapy in human ovarian cancer.

    PubMed

    Odunsi, Kunle; Matsuzaki, Junko; James, Smitha R; Mhawech-Fauceglia, Paulette; Tsuji, Takemasa; Miller, Austin; Zhang, Wa; Akers, Stacey N; Griffiths, Elizabeth A; Miliotto, Anthony; Beck, Amy; Batt, Carl A; Ritter, Gerd; Lele, Shashikant; Gnjatic, Sacha; Karpf, Adam R

    2014-01-01

    The cancer-testis/cancer-germline antigen NY-ESO-1 is a vaccine target in epithelial ovarian cancer (EOC), but its limited expression is a barrier to vaccine efficacy. As NY-ESO-1 is regulated by DNA methylation, we hypothesized that DNA methyltransferase (DNMT) inhibitors may augment NY-ESO-1 vaccine therapy. In agreement, global DNA hypomethylation in EOC was associated with the presence of circulating antibodies to NY-ESO-1. Pre-clinical studies using EOC cell lines showed that decitabine treatment enhanced both NY-ESO-1 expression and NY-ESO-1-specific CTL-mediated responses. Based on these observations, we performed a phase I dose-escalation trial of decitabine, as an addition to NY-ESO-1 vaccine and doxorubicin liposome (doxorubicin) chemotherapy, in 12 patients with relapsed EOC. The regimen was safe, with limited and clinically manageable toxicities. Both global and promoter-specific DNA hypomethylation occurred in blood and circulating DNAs, the latter of which may reflect tumor cell responses. Increased NY-ESO-1 serum antibodies and T cell responses were observed in the majority of patients, and antibody spreading to additional tumor antigens was also observed. Finally, disease stabilization or partial clinical response occurred in 6/10 evaluable patients. Based on these encouraging results, evaluation of similar combinatorial chemo-immunotherapy regimens in EOC and other tumor types is warranted.

  19. NY-ESO-1-specific immunological pressure and escape in a patient with metastatic melanoma.

    PubMed

    von Boehmer, Lotta; Mattle, Muriel; Bode, Peter; Landshammer, Alexandro; Schäfer, Carolin; Nuber, Natko; Ritter, Gerd; Old, Lloyd; Moch, Holger; Schäfer, Niklaus; Jäger, Elke; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    During cancer progression, malignant cells may evade immunosurveillance. However, evidence for immunological escape in humans is scarce. We report here the clinical course of a melanoma patient whose initial tumor was positive for the antigens NY-ESO-1, MAGE-C1, and Melan-A. Upon immunization with a recombinant vaccinia/fowlpox NY-ESO-1 construct, the patient experienced a mixed clinical response and spreading of the NY-ESO-1 epitopes in the CD4+ T cell compartment. After NY-ESO-1 protein + CpG immunization, the patient's anti-NY-ESO-1 IgG response increased. Over the following years, progressing lesions were resected and found to be NY-ESO-1-negative while being positive for MAGE-C1, Melan-A, and MHC-I. The fatal, inoperable brain metastasis was analyzed after his death and also proved to be NY-ESO-1-negative, while being positive for MAGE-C1 and Melan-A, as well as MHC-I. We propose that cancer control and cancer escape in this patient were governed by NY-ESO-1-specific immunological pressure. Our findings provide evidence for the existence of immunoediting and immunoescape in this cancer patient.

  20. 76 FR 60962 - Noise Exposure Map Update for Buffalo Niagara International Airport, Buffalo, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-30

    ... Country Road, Suite 446, Garden City, NY 11530, Telephone (516) 227-3815. SUPPLEMENTARY INFORMATION: This... Country Road, Suite 446, Garden City, 14225. Monday-Friday--9 a.m.-4 NY 11530. Monday-Friday--9 a.m.-4...

  1. NY-BR-1 Antigen Expression and anti-NY-BR-1 IgG in Egyptian Breast Cancer Patients: Clinicopathological and Prognostic Significance.

    PubMed

    Abu El-Nazar, Salma Y; Ghazy, Amany A; Ghoneim, Hossam E; Zoheir, Malak; Ahmed, Ahmed S; Sorour, Sally S; Abouelella, Amira M

    2015-01-01

    Breast cancer is the most common gynecological malignancy in the world. In Egypt, it ranks the first among female malignancies with incidence of 37.7%. Over the last decades, the integration of prognostic and predictive markers in treatment decisions has led to more individualized and optimized therapy. NY-BR-1 antigen has been shown to be frequently expressed in breast cancers. The study aimed to assess the tissue expression of NY-BR-1 antigen and serum IgG antibody to this antigen in Egyptian breast cancer females. The study was conducted on 60 females (10 healthy, 10 having benign breast lesions, 40 with malignant breast cancer). NY-BR-1 Ag expression was evaluated by immunohistochemistry and anti-NY-BR-1 IgG was assessed by ELISA. Results revealed a significant difference in NY-BR-1 Ag expression between benign and malignant breast cancer patients. There was a significant correlation between NY-BR-1 antigen expression and estrogen receptor's status (P = 0.019), stage of the disease (P = 0.008), menopausal status (P = 0.008), lymph node involvement (P = 0.022) and anti-NY-BR-1 IgG (P = 0.032) among the studied individuals. In addition, there was a statistically significant increase in anti-NY-BR-1 IgG O.D. results among malignant breast cancer group. It is correlated with tumor type (P < 0.001) and progesterone receptor status (P = 0.038). In conclusion, our work may represent a step towards identification of a new prognostic marker specific for breast cancer. PMID:26415367

  2. Two enzymes, TilS and HprT, can form a complex to function as a transcriptional activator for the cell division protease gene ftsH in Bacillus subtilis.

    PubMed

    Lin, Ta-Hui; Hu, Yi-Nei; Shaw, Gwo-Chyuan

    2014-01-01

    The FtsH protein is an ATP-dependent cytoplasmic membrane protease involved in the control of membrane protein quality, cell division and heat shock response in Bacillus subtilis and many other bacteria. TilS, the tRNA(Ile2) lysidine synthetase, is a tRNA-binding protein that can modify pre-tRNA(Ile2). HprT, the hypoxanthine-guanine phosphoribosyltransferase, is implicated in purine salvage. Both tilS and hprT are essential for cell viability of B. subtilis. In this report, by co-purification experiments and gel filtration analyses, we show that there is complex formation between co-expressed TilS and HprT. Electrophoretic mobility shift assays and in vitro transcription analyses demonstrated that the TilS/HprT complex functions as a specific DNA-binding protein that can stimulate ftsH transcription in vitro. Two regions located upstream of the ftsH promoter have been identified as the TilS/HprT-binding sites and shown to be required for TilS/HprT-dependent ftsH transcription in vitro and in vivo. Results from gel supershift assays support the notion that the TilS/HprT complex likely employs its distinct segments for interaction with these two distinct TilS/HprT-binding sites, respectively. In conclusion, we present the first evidence that bi-functional TilS and HprT can form a complex to function as a transcriptional activator to stimulate ftsH transcription.

  3. 33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Buffalo, N.Y.; use, administration, and navigation. 207.590 Section 207.590 Navigation and Navigable... Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation. (a) The term “canal... immediately to the Black Rock Lock, foot of Bridge Street, Buffalo, N.Y., telephone 876-5454. (k) Ferry...

  4. 33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Buffalo, N.Y.; use, administration, and navigation. 207.590 Section 207.590 Navigation and Navigable... Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation. (a) The term “canal... immediately to the Black Rock Lock, foot of Bridge Street, Buffalo, N.Y., telephone 876-5454. (k) Ferry...

  5. 33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Buffalo, N.Y.; use, administration, and navigation. 207.590 Section 207.590 Navigation and Navigable... Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation. (a) The term “canal... immediately to the Black Rock Lock, foot of Bridge Street, Buffalo, N.Y., telephone 876-5454. (k) Ferry...

  6. 33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Buffalo, N.Y.; use, administration, and navigation. 207.590 Section 207.590 Navigation and Navigable... Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation. (a) The term “canal... immediately to the Black Rock Lock, foot of Bridge Street, Buffalo, N.Y., telephone 876-5454. (k) Ferry...

  7. 33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Buffalo, N.Y.; use, administration, and navigation. 207.590 Section 207.590 Navigation and Navigable... Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation. (a) The term “canal... immediately to the Black Rock Lock, foot of Bridge Street, Buffalo, N.Y., telephone 876-5454. (k) Ferry...

  8. T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma

    PubMed Central

    Singh, Nathan; Kulikovskaya, Irina; Barrett, David M.; Binder-Scholl, Gwendolyn; Jakobsen, Bent; Martinez, Daniel; Pawel, Bruce; June, Carl H.; Kalos, Michael D.; Grupp, Stephan A.

    2016-01-01

    abstract The cancer-testis antigen NY-ESO-1 is expressed by many solid tumors and has limited expression by mature somatic tissues, making it a highly attractive target for tumor immunotherapy. Targeting NY-ESO-1 using engineered T cells has demonstrated clinical efficacy in the treatment of some adult tumors. Neuroblastoma is a significant cause of cancer mortality in children, and is a tumor type shown to be responsive to immunotherapies. We evaluated a large panel of primarily resected neuroblastoma samples and demonstrated that 23% express NY-ESO-1. After confirming antigen-specific activity of T cells genetically engineered to express an NY-ESO-1 directed high-affinity transgenic T cell receptor in vitro, we performed xenograft mouse studies assessing the efficacy of NY-ESO-1-targeted T cells in both localized and disseminated models of neuroblastoma. Disease responses were monitored by tumor volume measurement and in vivo bioluminescence. After delivery of NY-ESO-1 transgenic TCR T cells, we observed significant delay of tumor progression in mice bearing localized and disseminated neuroblastoma, as well as enhanced animal survival. These data demonstrate that NY-ESO-1 is an antigen target in neuroblastoma and that targeted T cells represent a potential therapeutic option for patients with neuroblastoma. PMID:26942053

  9. Expression of MAGE-A and NY-ESO-1 in Primary and Metastatic Cancers.

    PubMed

    Park, Tristen S; Groh, Eric M; Patel, Krishna; Kerkar, Sid P; Lee, Chyi-Chia Richard; Rosenberg, Steven A

    2016-01-01

    Melanoma-associated antigen-A (MAGE-A) and New York esophageal squamous cell cancer-1 (NY-ESO-1) are 2 cancer testis antigens (CTA) demonstrating potential for use in targeted immunotherapy. Clinical trials in melanoma and synovial sarcomas targeting these antigens in immune-based therapies have demonstrated durable tumor regression. Although protein expression of NY-ESO-1 has been assessed in a variety of cancer types, the expression of MAGE-A has not been studied in depth. In this study we analyzed MAGE-A and NY-ESO-1 expression in 314 melanoma specimens from 301 melanoma patients, 38 patients with squamous cell cancers and 111 patients with adenocarcinomas. Our results demonstrated higher expression of MAGE-A compared with NY-ESO-1 in melanomas (32% vs. 13%) and squamous cell carcinomas (45% vs. 7.9%), and higher expression of both CTAs in metastatic versus primary tumors. CTA expression in adenocarcinomas was low (MAGE-A: 10%, NY-ESO-1: 0.9%). In addition, we looked at concordance of expression among metastatic melanoma lesions within the same patient and found concordant expression in 38 of 47 patients for MAGE-A and 43 of 47 patients for NY-ESO-1. Our study demonstrated that the MAGE-A family may be of greater utility than NY-ESO-1 for targeted immunotherapy in a variety of cancer histologies, in particular metastatic melanomas and squamous cell carcinomas.

  10. 18.5% efficient AlOx/SiNy rear passivated industrial multicrystalline silicon solar cells

    NASA Astrophysics Data System (ADS)

    Qiao, Qi; Lu, Hongyan; Ge, Jian; Xi, Xi; Chen, Rulong; Yang, Jian; Zhu, Jingbing; Shi, Zhengrong; Chu, Junhao

    2014-06-01

    Due to the trend toward thinner and higher efficient crystalline silicon solar cells, excellent rear surface passivation and internal optical reflectance have become more and more important. Aluminum oxide (AlOx) capped with silicon nitride (SiNy), which is considered as one of the most promising candidates to achieve superior rear passivation and internal reflectance, has to date been mostly used for the rear side of p-type monocrystalline silicon (mono-Si) solar cells. In this paper, we have optimized rear AlOx/SiNy stacks deposited by industrial plasma enhanced chemical vapor deposition (PECVD) for multicrystalline silicon (mc-Si) passivated emitter and rear cells (PERC). Sufficient passivation activation effect from industrial fast-firing process and SiNy deposition process have been demonstrated, so the samples were not subjected to additional thermal treatment process in the cell fabrication flow. For rear AlOx/SiNy stack, it is shown that when PECVD AlOx is thicker than 40 nm, apparent blisters in fired AlOx deteriorate the cell performance, and the appropriate SiNy capping is N-rich SiNy with thickness of at least 180 nm. After process optimization with the least additional process steps, independently confirmed efficiency of 18.5% for Pluto-PERC with PECVD AlOx/SiNy rear passivation on standard 156 mm × 156 mm p-type mc-Si wafers has been achieved.

  11. A novel human-derived antibody against NY-ESO-1 improves the efficacy of chemotherapy.

    PubMed

    Gupta, Anurag; Nuber, Natko; Esslinger, Christoph; Wittenbrink, Mareike; Treder, Martin; Landshammer, Alexandro; Noguchi, Takuro; Kelly, Marcus; Gnjatic, Sacha; Ritter, Erika; von Boehmer, Lotta; Nishikawa, Hiroyoshi; Shiku, Hiroshi; Old, Lloyd; Ritter, Gerd; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    We investigated whether antibodies against intracellular tumor-associated antigens support tumor-specific immunity when administered together with a treatment that destroys the tumor. We propose that released antigens form immune complexes with the antibodies, which are then efficiently taken up by dendritic cells. We cloned the first human monoclonal antibodies against the Cancer/Testis (CT) antigen, NY-ESO-1. We tested whether the monoclonal anti-NY-ESO-1 antibody (12D7) facilitates cross-presentation of a NY-ESO-1-derived epitope by dendritic cells to human CD8+ T cells, and whether this results in the maturation of dendritic cells in vitro. We investigated the efficacy of 12D7 in combination with chemotherapy using BALB/c mice bearing syngeneic CT26 tumors that express intracellular NY-ESO-1. Human dendritic cells that were incubated with NY-ESO-1:12D7 immune complexes efficiently stimulated NY-ESO-1(157-165)/HLA-A2-specific human CD8+ T cells to produce interferon-γ, whereas NY-ESO-1 alone did not. Furthermore, the incubation of dendritic cells with NY-ESO-1:12D7 immune complexes resulted in the maturation of dendritic cells. Treatment of BALB/c mice that bear CT26/NY-ESO-1 tumors with 5-fluorouracil (5-FU) plus 12D7 was significantly more effective than chemotherapy alone. We propose systemic injection of monoclonal antibodies (mAbs) against tumor-associated antigens plus a treatment that promotes the local release of those antigens resulting in immune complex formation as a novel therapeutic modality for cancer. PMID:23390374

  12. A novel human-derived antibody against NY-ESO-1 improves the efficacy of chemotherapy

    PubMed Central

    Gupta, Anurag; Nuber, Natko; Esslinger, Christoph; Wittenbrink, Mareike; Treder, Martin; Landshammer, Alexandro; Noguchi, Takuro; Kelly, Marcus; Gnjatic, Sacha; Ritter, Erika; von Boehmer, Lotta; Nishikawa, Hiroyoshi; Shiku, Hiroshi; Old, Lloyd; Ritter, Gerd; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    We investigated whether antibodies against intracellular tumor-associated antigens support tumor-specific immunity when administered together with a treatment that destroys the tumor. We propose that released antigens form immune complexes with the antibodies, which are then efficiently taken up by dendritic cells. We cloned the first human monoclonal antibodies against the Cancer/Testis (CT) antigen, NY-ESO-1. We tested whether the monoclonal anti-NY-ESO-1 antibody (12D7) facilitates cross-presentation of a NY-ESO-1-derived epitope by dendritic cells to human CD8+ T cells, and whether this results in the maturation of dendritic cells in vitro. We investigated the efficacy of 12D7 in combination with chemotherapy using BALB/c mice bearing syngeneic CT26 tumors that express intracellular NY-ESO-1. Human dendritic cells that were incubated with NY-ESO-1:12D7 immune complexes efficiently stimulated NY-ESO-1157–165/HLA-A2-specific human CD8+ T cells to produce interferon-γ, whereas NY-ESO-1 alone did not. Furthermore, the incubation of dendritic cells with NY-ESO-1:12D7 immune complexes resulted in the maturation of dendritic cells. Treatment of BALB/c mice that bear CT26/NY-ESO-1 tumors with 5-fluorouracil (5-FU) plus 12D7 was significantly more effective than chemotherapy alone. We propose systemic injection of monoclonal antibodies (mAbs) against tumor-associated antigens plus a treatment that promotes the local release of those antigens resulting in immune complex formation as a novel therapeutic modality for cancer. PMID:23390374

  13. A novel human-derived antibody against NY-ESO-1 improves the efficacy of chemotherapy.

    PubMed

    Gupta, Anurag; Nuber, Natko; Esslinger, Christoph; Wittenbrink, Mareike; Treder, Martin; Landshammer, Alexandro; Noguchi, Takuro; Kelly, Marcus; Gnjatic, Sacha; Ritter, Erika; von Boehmer, Lotta; Nishikawa, Hiroyoshi; Shiku, Hiroshi; Old, Lloyd; Ritter, Gerd; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    We investigated whether antibodies against intracellular tumor-associated antigens support tumor-specific immunity when administered together with a treatment that destroys the tumor. We propose that released antigens form immune complexes with the antibodies, which are then efficiently taken up by dendritic cells. We cloned the first human monoclonal antibodies against the Cancer/Testis (CT) antigen, NY-ESO-1. We tested whether the monoclonal anti-NY-ESO-1 antibody (12D7) facilitates cross-presentation of a NY-ESO-1-derived epitope by dendritic cells to human CD8+ T cells, and whether this results in the maturation of dendritic cells in vitro. We investigated the efficacy of 12D7 in combination with chemotherapy using BALB/c mice bearing syngeneic CT26 tumors that express intracellular NY-ESO-1. Human dendritic cells that were incubated with NY-ESO-1:12D7 immune complexes efficiently stimulated NY-ESO-1(157-165)/HLA-A2-specific human CD8+ T cells to produce interferon-γ, whereas NY-ESO-1 alone did not. Furthermore, the incubation of dendritic cells with NY-ESO-1:12D7 immune complexes resulted in the maturation of dendritic cells. Treatment of BALB/c mice that bear CT26/NY-ESO-1 tumors with 5-fluorouracil (5-FU) plus 12D7 was significantly more effective than chemotherapy alone. We propose systemic injection of monoclonal antibodies (mAbs) against tumor-associated antigens plus a treatment that promotes the local release of those antigens resulting in immune complex formation as a novel therapeutic modality for cancer.

  14. NY-ESO-1 expression in hepatocellular carcinoma: A potential new marker for early recurrence after surgery.

    PubMed

    Xu, Heng; Gu, Na; Liu, Zhao-Bo; Zheng, Min; Xiong, Fang; Wang, Si-Ying; Li, Ning; Lu, Jun

    2012-01-01

    NY-ESO-1 belongs to the cancer testis antigens (CTA) family, and is identified in a variety of tumors. Certain studies have demonstrated that NY-ESO-1 predicts tumor recurrence and treatment response. No reports are currently available regarding the correlation between NY-ESO-1 and the recurrence of hepatocellular carcinoma (HCC) following surgery. The purpose of the present study was to evaluate the association between NY-ESO-1 and relapse of HCC and to explore the possible mechanisms for this correlation. A total of 120 HCC patients were analyzed for the expression of NY-ESO-1 by immunohistochemistry (IHC). A stable NY-ESO-1 over-expressed HepG2 cell line (ESO-HepG2) was established to determine the biological effects of NY-ESO-1 on cell proliferation, cell cycle and migration by using the xCELLigence DP system, flow cytometry and xCELLigence SP system. NY-ESO-1 was positive in 28 of 120 (23.3%) HCC tumor tissues. NY-ESO-1 was not detectable in adjacent normal liver tissues. A close correlation was found between NY-ESO-1 expression and the recurrence of HCC following surgery (P=0.007). Kaplan-Meier analysis showed a shorter recurrence-free survival (RFS) for patients positive for NY-ESO-1 (log-rank test, P=0.003). The Cox regression model demonstrated that NY-ESO-1 expression was a significant independent predictor for the recurrence of HCC following curative surgery (P=0.022). Compared with HepG2 cells, ESO-HepG2 cells have increased migration but not proliferation ability. In conclusion, NY-ESO-1 expression is associated with worse HCC outcome following surgery, and the mechanism for this finding may be that NY-ESO-1 increases tumor cell migration.

  15. 77 FR 49730 - Safety Zone; Antique Boat Show, Niagara River, Grand Island, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-17

    ... Safety Zone; Antique Boat Show, Niagara River, Grand Island, NY in the Federal Register (77 FR 13516). We... Mercurio, Chief of Waterway Management, U.S. Coast Guard Sector Buffalo; telephone 716-843-9343,...

  16. 75 FR 15739 - International Business Machines Corporation: Armonk, NY; Notice of Termination of Investigation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration International Business Machines Corporation: Armonk, NY; Notice of... Business Machines Corporation, Armonk, New York. The petitioning group of workers is covered by...

  17. 76 FR 43712 - Notice of Inventory Completion: American Museum of Natural History, New York, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-21

    ... National Park Service Notice of Inventory Completion: American Museum of Natural History, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The American Museum of Natural History... human remains may contact the American Museum of Natural History.......

  18. 75 FR 7030 - Dawson Metal Company, Inc., Industrial Division, Jamestown, NY; Notice of Affirmative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-16

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Dawson Metal Company, Inc., Industrial Division, Jamestown, NY; Notice... determination based on the finding that imports of precision sheet metal fabrication did not...

  19. 76 FR 4078 - Television Broadcasting Services; North Pole and Plattsburgh, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-24

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Television Broadcasting Services; North Pole and Plattsburgh, NY AGENCY: Federal... CFR Part 73 Television, Television broadcasting. For the reasons discussed in the preamble,...

  20. 75 FR 33329 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-11

    ... Dentistry, New York, NY. The human remains were removed from Lovelock Cave, Churchill County, NV. This... representing a minimum of one individual were removed from Lovelock Cave, Churchill County, NV. The...

  1. Three novel NY-ESO-1 epitopes bound to DRB1*0803, DQB1*0401 and DRB1*0901 recognized by CD4 T cells from CHP-NY-ESO-1-vaccinated patients.

    PubMed

    Mizote, Yu; Taniguchi, Taku; Tanaka, Kei; Isobe, Midori; Wada, Hisashi; Saika, Takashi; Kita, Shoichi; Koide, Yukari; Uenaka, Akiko; Nakayama, Eiichi

    2010-07-19

    Three novel NY-ESO-1 CD4 T cell epitopes were identified using PBMC obtained from patients who were vaccinated with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1). The restriction molecules were determined by antibody blocking and using various EBV-B cells with different HLA alleles as APC to present peptides to CD4 T cells. The minimal epitope peptides were determined using various N- and C-termini truncated peptides deduced from 18-mer overlapping peptides originally identified for recognition. Those epitopes were DRB1*0901-restricted NY-ESO-1 87-100, DQB1*0401-restricted NY-ESO-1 95-107 and DRB1*0803-restricted NY-ESO-1 124-134. CD4 T cells used to determine those epitope peptides recognized EBV-B cells or DC that were treated with recombinant NY-ESO-1 protein or NY-ESO-1-expressing tumor cell lysate, suggesting that the epitope peptides are naturally processed. These CD4 T cells showed a cytokine profile with Th1 characteristics. Furthermore, NY-ESO-1 87-100 peptide/HLA-DRB1*0901 tetramer staining was observed. Multiple Th1-type CD4 T cell responses are beneficial for inducing effective anti-tumor responses after NY-ESO-1 protein vaccination.

  2. NY-ESO-1 (CTAG1B) expression in mesenchymal tumors.

    PubMed

    Endo, Makoto; de Graaff, Marieke A; Ingram, Davis R; Lim, Simin; Lev, Dina C; Briaire-de Bruijn, Inge H; Somaiah, Neeta; Bovée, Judith V M G; Lazar, Alexander J; Nielsen, Torsten O

    2015-04-01

    New York esophageal squamous cell carcinoma 1 (NY-ESO-1, CTAG1B) is a cancer-testis antigen and currently a focus of several targeted immunotherapeutic strategies. We performed a large-scale immunohistochemical expression study of NY-ESO-1 using tissue microarrays of mesenchymal tumors from three institutions in an international collaboration. A total of 1132 intermediate and malignant and 175 benign mesenchymal lesions were enrolled in this study. Immunohistochemical staining was performed on tissue microarrays using a monoclonal antibody for NY-ESO-1. Among mesenchymal tumors, myxoid liposarcomas showed the highest positivity for NY-ESO-1 (88%), followed by synovial sarcomas (49%), myxofibrosarcomas (35%), and conventional chondrosarcomas (28%). Positivity of NY-ESO-1 in the remaining mesenchymal tumors was consistently low, and no immunoreactivity was observed in benign mesenchymal lesions. On the basis of these findings, nearly 90% of myxoid liposarcomas, as well as a significant proportion of synovial sarcomas, myxofibrosarcomas, and conventional chondrosarcomas are good candidates for immunotherapy targeting NY-ESO-1.

  3. Sediment chemistries and chironomid deformities in the Buffalo River (NY)

    SciTech Connect

    Stewart, K.M.; Diggins, T.P.

    1994-12-31

    The authors examined the surficial sediment chemistry (heavy metals) and the frequency of chironomid (Diptera) larvae mouthpart deformities from multiple PONAR grabs samples at each of 20 sites along the Buffalo River (NY) area of concern (AOC). Because of the potential for patchy invertebrate distribution and high variance in sediment chemistry, repeated spatial and temporal sampling is important to obtain a better integrated picture of contamination in rivers. The findings suggest that the Buffalo River has one of the highest percentages of deformed chironomids in AOC`s of the Great Lakes basin. One river site that was traditionally thought to be a chemical hot spot was less contaminated than another downstream section. At another site, sediment concentrations for V., Mn and AS appeared to be strongly associated with the proximity of combined sewer overflows from a region which is primarily residential. Interestingly, a demonstration project of the US Army Corps of Engineers, during which three types of dredges were used to carefully remove upper sediments from two different short reaches along the river, seemed to have no significant impact on proximate sediment chemistries or biota.

  4. Antibody responses against NY-ESO-1 and HER2 antigens in patients vaccinated with combinations of cholesteryl pullulan (CHP)-NY-ESO-1 and CHP-HER2 with OK-432.

    PubMed

    Aoki, Masatoshi; Ueda, Shugo; Nishikawa, Hiroyoshi; Kitano, Shigehisa; Hirayama, Michiko; Ikeda, Hiroaki; Toyoda, Hideki; Tanaka, Kyosuke; Kanai, Michiyuki; Takabayashi, Arimichi; Imai, Hiroshi; Shiraishi, Taizo; Sato, Eiichi; Wada, Hisashi; Nakayama, Eiichi; Takei, Yoshiyuki; Katayama, Naoyuki; Shiku, Hiroshi; Kageyama, Shinichi

    2009-11-16

    Combination vaccines of the NY-ESO-1 protein complexed with cholesteryl pullulan (CHP), CHP-NY-ESO-1, and the truncated 146HER2 protein with CHP, CHP-HER2, were subcutaneously administered with the immuno-adjuvant OK-432 to eight esophageal cancer patients. Vaccination was well-tolerated. NY-ESO-1- and HER2-specific antibody responses were analyzed using the patients' sera and samples from previous single CHP-NY-ESO-1 or CHP-HER2 vaccine trial. The responses to NY-ESO-1 in the combination vaccine study were comparable to the single vaccine. For responses to HER2, there were fewer antibody responses in the combination vaccines. Although there were marked individual variations in the antibody responses to the NY-ESO-1 and HER2 antigens, the reaction patterns to these antigens were comparable within each patient. Antibodies to OK-432 were not augmented. Protein cancer vaccines targeting multiple antigens are feasible.

  5. First simultaneous measurements of Na and K thermospheric layers along with TILs from Arecibo

    NASA Astrophysics Data System (ADS)

    Raizada, Shikha; Brum, C. M.; Tepley, C. A.; Lautenbach, Jens; Friedman, J. S.; Mathews, John D.; Djuth, F. T.; Kerr, Caitlin

    2015-12-01

    This work presents the first simultaneous observations of Na and K between 120 and 150 km altitudes along with ionospheric tidal ion layers (TILs) obtained on 30 January 2006 data from Arecibo. The latter displays an average downward phase velocity of ~14.7 ms-1. However, the neutral layers descend together at a much slower velocity of about ~0.69 ms-1. This indicates that thermospheric atomic metal layers are not necessarily associated with TILs. The ratio of the average Na/K abundances in thermosphere is ~35.5 as compared to 150 in the main layer (80-105 km). The long lifetimes of ions at ~140 km implies that neutral layers cannot result from direct neutralization of metal ions in the TILs. We investigate different mechanisms that can deposit neutral atoms at thermospheric altitudes.

  6. Aircraft borne combined measurements of the Fukushima radionuclide Xe-133 and fossil fuel combustion generated pollutants in the TIL - Implications for Cyclone induced lift and TIL physical-chemical processes

    NASA Astrophysics Data System (ADS)

    Arnold, Frank; Schlager, Hans; Simgen, Hardy; Aufmhoff, Heinfried; Baumann, Robert; Lindemann, Sigfried; Rauch, Ludwig; Kaether, Frank; Pirjolla, Liisa; Schumann, Ulrich

    2013-04-01

    The radionuclide Xe-133, released by the March 2011 nuclear disaster at Fukushima/Daiichi (hereafter FD), represents an ideal tracer for atmospheric transport. We report the, to our best knowledge, only aircraft borne measurements of FD Xe-133 in the Tropopause Inversion Layer (TIL), indicating rapid lift of Xe-133 rich planetary boundary layer air to the TIL. On the same research aircraft (FALCON), we have also conducted on-line measurements of fossil fuel combustion generated pollutant gases (SO2, NOx, HNO3,NOy), which were found to have increased concentrations in the TIL. In addition, we have conducted supporting model simulations of transport, chemical processes, and aerosol processes. Our investigations reveal a potentially important influence of East-Asian cyclone induced pollutants transport to the TIL, particularly influencing aerosol formation in the TIL.

  7. Strong and rapid induction of osteoblast differentiation by Cbfa1/Til-1 overexpression for bone regeneration.

    PubMed

    Kojima, Hiroko; Uemura, Toshimasa

    2005-01-28

    Core binding factor alpha-1 (Cbfa1), known as an essential transcription factor for osteogenic lineage, has two major N-terminal isoforms: Pebp2alphaA and Til-1. To study the roles of these isoforms in bone regeneration, we applied an adenoviral vector carrying their genes to transduce primary osteoprogenitor cells in vitro and in vivo. Overexpression of the two isoforms induced rapid and marked osteoblast differentiation, with Til-1 being more effective in vitro, by examination of the alkaline phosphatase activity, calcium content, and Alizarin red staining. Til-1 overexpressing cells/porous ceramic composites were transplanted into subcutaneous and bone defect sites in Fischer rats (cultured bone transplantation model) and markedly affected in vivo bone formation and osteoblast markers. The results demonstrated that the reconstitution of bone tissues, such as cortical bone and trabecular bone was accelerated by implantation of Til-1 overexpressing cells/porous ceramic composites. Moreover, the new bone formation by Til-1 overexpression appeared to reflect replacement of new bone within the implant boundaries. To ascertain whether implanted Cbfa1 overexpressing cells could differentiate into osteogenic cells to create bone or whether it stimulated the surrounding recipient tissue to regenerate bone, implanted male donor cells were visualized by fluorescent in situ hybridization analysis. The proportion of implanted cells in the presumptive bone forming region was over 80% and did not change throughout from 3 days to 8 weeks after implantation. These findings suggested that the newly formed bone in the porous area of the scaffold is mostly produced by the implanted donor cells or their derived cells, effectively by Til-1 overexpression.

  8. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination.

    PubMed

    Kawada, Junji; Wada, Hisashi; Isobe, Midori; Gnjatic, Sacha; Nishikawa, Hiroyoshi; Jungbluth, Achim A; Okazaki, Nami; Uenaka, Akiko; Nakamura, Yurika; Fujiwara, Shinichi; Mizuno, Naoaki; Saika, Takashi; Ritter, Erika; Yamasaki, Makoto; Miyata, Hiroshi; Ritter, Gerd; Murphy, Roger; Venhaus, Ralph; Pan, Linda; Old, Lloyd J; Doki, Yuichiro; Nakayama, Eiichi

    2012-02-01

    NY-ESO-1 is a prototypic cancer/testis antigen. In a recent phase I clinical trial, we vaccinated 13 patients bearing NY-ESO-1-expressing tumors with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1) and showed efficient induction of NY-ESO-1 antibody, and CD4 and CD8 T cell responses using peripheral blood from the patients. In our study, we analyzed heteroclitic serological responses in those patients after vaccination. Serological response against 11 tumor antigens including MAGE-A1, MAGE-A3, MAGE-A4, CT7/MAGEC1, CT10/MAGEC2, CT45, CT46/HORMAD1, SOX2, SSX2, XAGE1B and p53 was examined by enzyme-linked immunosorbent assay (ELISA) using sera from ten vaccinated patients. Expression of tumor antigens was determined by reverse transcription-polymerase chain reaction or immunohistochemistry. Eight of nine patients who showed antibody responses against NY-ESO-1 also showed an antibody response against at least 1 of these 11 tumor antigens after vaccination. In one patient, seven tumor antigens were recognized. Specificity analysis of the antibody response by ELISA using control recombinant proteins and synthetic peptides and by Western blot showed that the response was not against His6-tag and/or bacterial products included in a preparation of CHP-NY-ESO-1 used for vaccination. Thus, heteroclitic serological responses appear to be indicative of the overall immune response against the tumor, and their analysis could be useful for immune monitoring in cancer vaccine.

  9. NY-ESO-1 expression in meningioma suggests a rationale for new immunotherapeutic approaches.

    PubMed

    Baia, Gilson S; Caballero, Otavia L; Ho, Janelle S Y; Zhao, Qi; Cohen, Tzeela; Binder, Zev A; Salmasi, Vafi; Gallia, Gary L; Quinones-Hinojosa, Alfredo; Olivi, Alessandro; Brem, Henry; Burger, Peter; Strausberg, Robert L; Simpson, Andrew J G; Eberhart, Charles G; Riggins, Gregory J

    2013-11-01

    Meningiomas are the most common primary intracranial tumors. Surgical resection remains the treatment of choice for these tumors. However, a significant number of tumors are not surgically accessible, recur, or become malignant, necessitating the repetition of surgery and sometimes radiation. Chemotherapy is rarely used and is generally not recognized as an effective treatment. Cancer/testis (CT) genes represent a unique class of genes, which are expressed by germ cells, normally silenced in somatic cells, but activated in various cancers. CT proteins can elicit spontaneous immune responses in patients with cancer and this feature makes them attractive targets for immunotherapy-based approaches. We analyzed mRNA expression of 37 testis-restricted CT genes in a discovery set of 18 meningiomas by reverse transcription PCR. The overall frequency of expression of CT genes ranged from 5.6% to 27.8%. The most frequently expressed was NY-ESO-1, in 5 patients (27.8%). We subsequently analyzed NY-ESO-1 protein expression in a larger set of meningiomas by immunohistochemistry and found expression in 108 of 110 cases. In some cases, NY-ESO-1 expression was diffused and homogenous, but in most instances it was heterogeneous. Importantly, NY-ESO-1 expression was positively correlated with higher grade and patients presenting with higher levels of NY-ESO-1 staining had significantly worse disease-free and overall survival. We have also shown that NY-ESO-1 expression may lead to humoral immune response in patients with meningioma. Considering the limited treatment options for patients with meningioma, the potential of NY-ESO-1-based immunotherapy should be explored.

  10. CTLA-4 blockade enhances polyfunctional NY-ESO-1 specific T cell responses in metastatic melanoma patients with clinical benefit.

    PubMed

    Yuan, Jianda; Gnjatic, Sacha; Li, Hao; Powel, Sarah; Gallardo, Humilidad F; Ritter, Erika; Ku, Geoffrey Y; Jungbluth, Achim A; Segal, Neil H; Rasalan, Teresa S; Manukian, Gregor; Xu, Yinyan; Roman, Ruth-Ann; Terzulli, Stephanie L; Heywood, Melanie; Pogoriler, Evelina; Ritter, Gerd; Old, Lloyd J; Allison, James P; Wolchok, Jedd D

    2008-12-23

    Blockade of inhibitory signals mediated by cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) has been shown to enhance T cell responses and induce durable clinical responses in patients with metastatic melanoma. The functional impact of anti-CTLA-4 therapy on human immune responses is still unclear. To explore this, we analyzed immune-related adverse events and immune responses in metastatic melanoma patients treated with ipilimumab, a fully human anti-CTLA-4 monoclonal antibody. Fifteen patients were selected on the basis of availability of suitable specimens for immunologic monitoring, and eight of these showed evidence of clinical benefit. Five of the eight patients with evidence of clinical benefit had NY-ESO-1 antibody, whereas none of seven clinical non-responders was seropositive for NY-ESO-1. All five NY-ESO-1 seropositive patients had clearly detectable CD4(+) and CD8(+) T cells against NY-ESO-1 following treatment with ipilimumab. One NY-ESO-1 seronegative clinical responder also had a NY-ESO-1 CD4(+) and CD8(+) T cell response, possibly related to prior vaccination with NY-ESO-1. Among five clinical non-responders analyzed, only one had a NY-ESO-1 CD4(+) T cell response and this patient did not have detectable anti-NY-ESO-1 antibody. Overall, NY-ESO-1-specific T cell responses increased in frequency and functionality during anti-CTLA-4 treatment, revealing a polyfunctional response pattern of IFN-gamma, MIP-1beta and TNF-alpha. We therefore suggest that CTLA-4 blockade enhanced NY-ESO-1 antigen-specific B cell and T cell immune responses in patients with durable objective clinical responses and stable disease. These data provide an immunologic rationale for the efficacy of anti-CTLA-4 therapy and call for immunotherapeutic designs that combine NY-ESO-1 vaccination with CTLA-4 blockade.

  11. Expression and clinical relevance of NY-ESO-1, MAGE-1 and MAGE-3 in neuroblastoma.

    PubMed

    Söling, A; Schurr, P; Berthold, F

    1999-01-01

    Human genes NY-ESO-1, MAGE-1 and MAGE-3 code for antigens which are expressed in malignancies of various histological types but not in normal tissues except testis. These antigens might therefore represent potential targets for specific immunotherapy. We studied the expression of genes NY-ESO-1, MAGE-1 and MAGE-3 in 98 neuroblastoma tumors by reverse transcription-polymerase chain reaction (RT-PCR). MAGE-1 was expressed in 66%, NY-ESO-1 in 36% and MAGE-3 in 33% of the tumors. NY-ESO-1 gene expression was associated with age older than one year (p = 0.017), more differentiated tumor histology (p = 0.044), elevated urinary vanillylmandelic acid (VMA, p = 0.018) and normal serum ferritin levels (p = 0.023). MAGE-1 expression correlated significantly with normal serum ferritin levels (p = 0.009) and absence of MycN amplification (p = 0.007) while MAGE-3 expression was associated with absence of metastasis (p = 0.027). We conclude that approximately 70% of the neuroblastoma tumors express at least one of the genes coding for NY-ESO-1, MAGE-1 or -3, respectively.

  12. A phase I study of vaccination with NY-ESO-1f peptide mixed with Picibanil OK-432 and Montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    PubMed

    Kakimi, Kazuhiro; Isobe, Midori; Uenaka, Akiko; Wada, Hisashi; Sato, Eiichi; Doki, Yuichiro; Nakajima, Jun; Seto, Yasuyuki; Yamatsuji, Tomoki; Naomoto, Yoshio; Shiraishi, Kenshiro; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Oka, Mikio; Pan, Linda; Hoffman, Eric W; Old, Lloyd J; Nakayama, Eiichi

    2011-12-15

    We conducted a phase I clinical trial of a cancer vaccine using a 20-mer NY-ESO-1f peptide (NY-ESO-1 91-110) that includes multiple epitopes recognized by antibodies, and CD4 and CD8 T cells. Ten patients were immunized with 600 μg of NY-ESO-1f peptide mixed with 0.2 KE Picibanil OK-432 and 1.25 ml Montanide ISA-51. Primary end points of the study were safety and immune response. Subcutaneous injection of the NY-ESO-1f peptide vaccine was well tolerated. Vaccine-related adverse events observed were fever (Grade 1), injection-site reaction (Grade 1 or 2) and induration (Grade 2). Vaccination with the NY-ESO-1f peptide resulted in an increase or induction of NY-ESO-1 antibody responses in nine of ten patients. The sera reacted with recombinant NY-ESO-1 whole protein as well as the NY-ESO-1f peptide. An increase in CD4 and CD8 T cell responses was observed in nine of ten patients. Vaccine-induced CD4 and CD8 T cells responded to NY-ESO-1 91-108 in all patients with various HLA types with a less frequent response to neighboring peptides. The findings indicate that the 20-mer NY-ESO-1f peptide includes multiple epitopes recognized by CD4 and CD8 T cells with distinct specificity. Of ten patients, two with lung cancer and one with esophageal cancer showed stable disease. Our study shows that the NY-ESO-1f peptide vaccine was well tolerated and elicited humoral, CD4 and CD8 T cell responses in immunized patients.

  13. Temperature-induced lipocalin (TIL) is translocated under salt stress and protects chloroplasts from ion toxicity.

    PubMed

    Abo-Ogiala, Atef; Carsjens, Caroline; Diekmann, Heike; Fayyaz, Payam; Herrfurth, Cornelia; Feussner, Ivo; Polle, Andrea

    2014-02-15

    Temperature-induced lipocalins (TIL) have been invoked in the defense from heat, cold and oxidative stress. Here we document a function of TIL for basal protection from salinity stress. Heterologous expression of TIL from the salt resistant poplar Populus euphratica did not rescue growth but prevented chlorophyll b destruction in salt-exposed Arabidopsis thaliana. The protein was localized to the plasma membrane but was re-translocated to the symplast under salt stress. The A. thaliana knock out and knock down lines Attil1-1 and Attil1-2 showed stronger stress symptoms and stronger chlorophyll b degradation than the wildtype (WT) under excess salinity. They accumulated more chloride and sodium in chloroplasts than the WT. Chloroplast chloride accumulation was found even in the absence of salt stress. Since lipocalins are known to bind regulatory fatty acids of channel proteins as well as iron, we suggest that the salt-induced trafficking of TIL may be required for protection of chloroplasts by affecting ion homeostasis.

  14. Nobel Prize centenary: Robert Bárány and the vestibular system.

    PubMed

    Lopez, Christophe; Blanke, Olaf

    2014-11-01

    The hundredth anniversary of Robert Bárány's Nobel Prize in Medicine offers the opportunity to highlight the importance of his discoveries on the physiology and pathophysiology of the vestibular organs. Bárány developed the method of caloric vestibular stimulation that revolutionized the investigation of the semicircular canals and that is still widely used today. Caloric vestibular stimulation launched experimental vestibular research that was relevant to comprehend the evolution of human locomotion, and Bárány's tests continue to be used in neuroscience to understand the influence of vestibular signals on bodily perceptions, cognition and emotions. Only during the last 20 years has caloric vestibular stimulation been merged with brain imaging to localize the human vestibular cortex. PMID:25517362

  15. Gallium nitride surface protection during RTA annealing with a GaOxNy cap-layer

    NASA Astrophysics Data System (ADS)

    Khalfaoui, Wahid; Oheix, T.; Cayrel, F.; Benoit, R.; Yvon, A.; Collard, E.; Alquier, D.

    2016-04-01

    Gallium nitride (GaN) is generally considered a good candidate for power electronic devices such as Schottky barrier diodes (SBDs). Nevertheless, GaN has a strong sensitivity to high temperature treatments and a cap-layer is mandatory to protect the material surface during annealing at high temperature such as post-implantation treatments. In this work, an oxidized gallium nitride layer (GaOxNy) was generated with Oxford PECVD equipment using a N2O plasma treatment to protect the GaN surface during a rapid thermal annealing (RTA), in the range of 1000 °C-1150 °C for a few minutes. Before annealing, c-TLM patterns were processed on the GaOxNy/GaN sample to characterize its sheet resistance. After the N2O plasma treatment, the sample exhibited lower sheet resistance, indicating a better n-type conduction of the GaOxNy layer due to an excess of free carriers, compared to the as-grown GaN layer. The GaOxNy/GaN surface was then annealed at 1150 °C for 3 min and observed through AFM imaging. The surface exhibited a good quality with a low roughness, nevertheless, a low density of small hexagonal pits appeared after annealing. Finally, studies to determine an efficient etching process of the GaOxNy cap-layer were conducted using both chemical and physical approaches. We observed that efficient etching of the layer was achieved using a heated hydrofluoridric acid (HF 25%) solution. To conclude, GaOxNy has proved to be an efficient cap-layer for GaN protection at high temperature.

  16. 77 FR 12829 - Bounce Energy NY, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-02

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Bounce Energy NY, LLC; Supplemental Notice That Initial Market- Based Rate...-referenced proceeding of Bounce Energy NY, LLC's application for market-based rate authority, with...

  17. 75 FR 35016 - EDF Industrial Power Services (NY), LLC; Supplemental Notice That Initial Market-Based Rate...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission EDF Industrial Power Services (NY), LLC; Supplemental Notice That Initial... supplemental notice in the above-referenced proceeding of EDF Industrial Power Services (NY), LLC's...

  18. 33 CFR 162.20 - Flushing Bay near La Guardia Airport, Flushing, N.Y.; restricted area.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Flushing Bay near La Guardia Airport, Flushing, N.Y.; restricted area. 162.20 Section 162.20 Navigation and Navigable Waters COAST... NAVIGATION REGULATIONS § 162.20 Flushing Bay near La Guardia Airport, Flushing, N.Y.; restricted area....

  19. 33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y. 110.84 Section 110.84 Navigation and Navigable Waters COAST GUARD... Channel opposite foot of Porter Avenue, Buffalo, N.Y. An area extending northwesterly between Black...

  20. 40 CFR 721.7600 - Alkyl(heterocyclicyl) phe-ny-lazohetero monocyclic poly-one (generic name).

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Alkyl(heterocyclicyl) phe-ny... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.7600 Alkyl(heterocyclicyl) phe-ny... reporting. (1) The chemical substance alkyl (heter-o-cyc-licyl) phenyl-azo-he-tero mono-cyclic polyone...

  1. 40 CFR 721.7600 - Alkyl(heterocyclicyl) phe-ny-lazohetero monocyclic poly-one (generic name).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Alkyl(heterocyclicyl) phe-ny... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.7600 Alkyl(heterocyclicyl) phe-ny... reporting. (1) The chemical substance alkyl (heter-o-cyc-licyl) phenyl-azo-he-tero mono-cyclic polyone...

  2. 40 CFR 721.7600 - Alkyl(heterocyclicyl) phe-ny-lazohetero monocyclic poly-one (generic name).

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Alkyl(heterocyclicyl) phe-ny... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.7600 Alkyl(heterocyclicyl) phe-ny... reporting. (1) The chemical substance alkyl (heter-o-cyc-licyl) phenyl-azo-he-tero mono-cyclic polyone...

  3. 40 CFR 721.7600 - Alkyl(heterocyclicyl) phe-ny-lazohetero monocyclic poly-one (generic name).

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Alkyl(heterocyclicyl) phe-ny... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.7600 Alkyl(heterocyclicyl) phe-ny... reporting. (1) The chemical substance alkyl (heter-o-cyc-licyl) phenyl-azo-he-tero mono-cyclic polyone...

  4. 40 CFR 721.7600 - Alkyl(heterocyclicyl) phe-ny-lazohetero monocyclic poly-one (generic name).

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Alkyl(heterocyclicyl) phe-ny... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.7600 Alkyl(heterocyclicyl) phe-ny... reporting. (1) The chemical substance alkyl (heter-o-cyc-licyl) phenyl-azo-he-tero mono-cyclic polyone...

  5. 75 FR 19644 - West Oaks Energy NY/NE, LP; Supplemental Notice That Initial Market-Based Rate Filing Includes...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission West Oaks Energy NY/NE, LP; Supplemental Notice That Initial Market-Based... supplemental notice in the above-referenced proceeding of West Oaks Energy NY/NE LP's application for...

  6. 76 FR 43259 - Foreign-Trade Zone 109-Watertown, NY, Application for Manufacturing Authority, North American...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-20

    ..., North American Tapes, LLC, (Textile Athletic Tape), Watertown, NY A request has been submitted to the...) with textile backing material for the U.S. market and export. The manufacturing process under FTZ... textile fabrics (duty rates: 10.5, 14.9%). The facility can produce up to 12.5 million square meters...

  7. 78 FR 36242 - Notice of Intent To Repatriate Cultural Items: New York State Museum, Albany, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-17

    ... discoidal shell beads, 1 tubular bone bead, 1 stone bead, 1 perforated brass child's thimble, 1 small crescent-shaped shell bead, 1 small lead bird figure, and 2 perforated triangular brass projectile points... perforated triangular brass projectile points. From Green Island in Albany County, NY, the 1...

  8. 33 CFR 165.T01-0727 - Regulated Navigation Area; Arthur Kill, NY and NJ.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (NAD 83). (b) Regulations. (1) The general regulations contained in 33 CFR 165.13 apply. (2) All... requirements through VTS measures, as per 33 CFR 161.11. (9) Suspension of enforcement: the Captain of the Port... Kill, NY and NJ. 165.T01-0727 Section 165.T01-0727 Navigation and Navigable Waters COAST...

  9. 33 CFR 165.T01-0727 - Regulated Navigation Area; Arthur Kill, NY and NJ.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (NAD 83). (b) Regulations. (1) The general regulations contained in 33 CFR 165.13 apply. (2) All... requirements through VTS measures, as per 33 CFR 161.11. (9) Suspension of enforcement: the Captain of the Port... Kill, NY and NJ. 165.T01-0727 Section 165.T01-0727 Navigation and Navigable Waters COAST...

  10. APPLICATION OF EMAP METHODS AND INDICATORS TO THE NY/NJ HARBOR

    EPA Science Inventory

    The Comprehensive Conservation and Management Plan (CCMP) for the NY/NJ Harbor requires specific management actions to maintain and restore the Harbor environment. It also specifies that the progress of these management actions on the improvement of sediment quality and biologic...

  11. 77 FR 42640 - Safety Zone; Can-Am Festival Fireworks, Black River Bay, Sackets Harbor, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-20

    ... Waterways Management, U.S. Coast Guard Sector Buffalo; telephone 716- 843-9343, email SectorBuffaloMarine... Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking A... Sackets ] Harbor, NY. The Captain of the Port Buffalo has determined that fireworks launched proximate...

  12. 78 FR 33972 - Safety Zone; RXR Sea Faire Celebration Fireworks, Glen Cove, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-06

    ... DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking A... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; RXR Sea Faire Celebration Fireworks, Glen... Cove, NY for a fireworks display. This temporary safety zone is necessary to protect spectators...

  13. 75 FR 13454 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-22

    ... Federal Register (73 FR 3316). Public Meeting We do not plan to hold a public meeting. But you may submit...-2009-0520) in the Federal Register (74 FR 51243). No comments or requests for meetings were received... Championships, Huntington Bay, NY AGENCY: Coast Guard, DHS. ACTION: Supplemental Notice of proposed...

  14. FIELD PERFORMANCE OF ADVANCED TECHNOLOGY WOODSTOVES IN GLEN FALLS, NY, 1988-89 - VOLUME I

    EPA Science Inventory

    The report gives results of an evaluation of particulate emission trends for three models of catalytic and two models of non- catalytic woodstoves under in-home burning conditions during the 1988-89 heating season in Glens Falls, NY. he results (averaging 9.4 g/h and 9.4 g/kg) sh...

  15. 77 FR 42644 - Safety Zone; Canal Fest of the Tonawandas, Erie Canal, Tonawanda, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-20

    ... Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking A... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Canal Fest of the Tonawandas, Erie Canal... establishing a temporary safety zone on the Erie Canal, Tonawanda, NY. This safety zone is intended to...

  16. 33 CFR 110.156 - Randall Bay, Freeport, Long Island, N.Y.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Randall Bay, Freeport, Long Island, N.Y. 110.156 Section 110.156 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND....Y. (a) The anchorage grounds. Southward of a line 312 feet south of and parallel to the south...

  17. SOURCE CHARACTERIZATION AND CONTROL TECHNOLOGY ASSESSMENT OF METHYLENE CHLORIDE EMISSIONS FROM EASTMAN KODAK COMPANY, ROCHESTER, NY

    EPA Science Inventory

    The report gives results of an assessment of potential control technologies for methylene chloride (also known as dichloromethane or DCM) emission sources at Eastman Kodak Company's Kodak Park facility in Rochester, NY. DCM is a solvent used by Kodak in the manufacture of cellulo...

  18. 75 FR 59966 - Safety Zone; New York Air Show at Jones Beach State Park, Wantagh, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ... over the Atlantic Ocean off of Jones Beach State Park. DATES: This rule is effective October 29, 2010... Show at Jones Beach State Park, Atlantic Ocean off of Jones Beach, Wantagh, NY, in the Federal Register... aircraft over a specified area of the Atlantic Ocean off of Jones Beach State Park. The safety zone...

  19. 75 FR 10547 - CSX Transportation, Inc.-Abandonment Exemption-in Jefferson County, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... Surface Transportation Board CSX Transportation, Inc.--Abandonment Exemption--in Jefferson County, NY CSX... Abandonments to abandon a 0.24-mile rail line, known as the Roe Feed Industrial Track, on its Northern Region... condition to this exemption, any employee adversely affected by the abandonment shall be protected...

  20. 76 FR 31008 - CSX Transportation, Inc.-Abandonment Exemption-in Erie County, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-27

    ... Surface Transportation Board CSX Transportation, Inc.--Abandonment Exemption--in Erie County, NY CSX... Abandonments to abandon an approximately 0.56-mile rail line on its Northern Region, Albany Division, Buffalo... abandonment shall be protected under Oregon Short Line Railroad--Abandonment Portion Goshen Branch...

  1. 77 FR 51614 - CSX Transportation, Inc.-Abandonment Exemption-in Niagara County, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-24

    ... Surface Transportation Board CSX Transportation, Inc.--Abandonment Exemption--in Niagara County, NY CSX... Abandonments to abandon a 0.1-mile rail line on its Northern Region, Albany Division, Niagara Subdivision... condition to this exemption, any employee adversely affected by the abandonment shall be protected...

  2. 76 FR 51470 - CSX Transportation, Inc.-Abandonment Exemption-In Oswego County, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-18

    ... Surface Transportation Board CSX Transportation, Inc.--Abandonment Exemption--In Oswego County, NY CSX... Abandonments to abandon 2 formerly connected rail lines in CSXT's Northern Region, Albany Division, Fulton... affected by the abandonment shall be protected under Oregon Short Line Railroad--Abandonment Portion...

  3. 33 CFR 100.106 - Freeport Grand Prix, Long Beach, NY.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Freeport Grand Prix, Long Beach... Beach, NY. (a) Regulated area. The regulated area is a trapezoidal area on the coastal Atlantic waters of Long Island to the south of Long Beach, New York. The regulated area is one and one quarter...

  4. 33 CFR 100.106 - Freeport Grand Prix, Long Beach, NY.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Freeport Grand Prix, Long Beach... Beach, NY. (a) Regulated area. The regulated area is a trapezoidal area on the coastal Atlantic waters of Long Island to the south of Long Beach, New York. The regulated area is one and one quarter...

  5. 33 CFR 110.156 - Randall Bay, Freeport, Long Island, N.Y.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Island, N.Y. 110.156 Section 110.156 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.156 Randall Bay, Freeport, Long Island, N... available, may be assigned to any vessel by the Captain of the Port of Long Island Sound. (2) The Captain...

  6. 77 FR 34894 - Safety Zone; Bostock 50th Anniversary Fireworks, Long Island Sound; Manursing Island, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-12

    ... Acronyms DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking A... Federal Register (73 FR 3316). 4. Public Meeting We do not now plan to hold a public meeting. But you may... Island Sound; Manursing Island, NY AGENCY: Coast Guard, DHS. ACTION: Notice of proposed...

  7. 33 CFR 110.156 - Randall Bay, Freeport, Long Island, N.Y.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Island, N.Y. 110.156 Section 110.156 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.156 Randall Bay, Freeport, Long Island, N... available, may be assigned to any vessel by the Captain of the Port of Long Island Sound. (2) The Captain...

  8. 78 FR 31454 - Drawbridge Operation Regulation; Hudson River, Troy and Green Island, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-24

    ... Security FR Federal Register NPRM Notice of Proposed Rulemaking Sec. Section Symbol U.S.C. United States... regarding our public dockets in the January 17, 2008, issue of the Federal Register (73 FR 3316). 4. Public... Green Island, NY AGENCY: Coast Guard, DHS. ACTION: Notice of proposed rulemaking. SUMMARY: The...

  9. 33 CFR 110.156 - Randall Bay, Freeport, Long Island, N.Y.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Randall Bay, Freeport, Long Island, N.Y. 110.156 Section 110.156 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.156 Randall Bay, Freeport, Long Island,...

  10. 33 CFR 110.156 - Randall Bay, Freeport, Long Island, N.Y.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Island, N.Y. 110.156 Section 110.156 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.156 Randall Bay, Freeport, Long Island, N... available, may be assigned to any vessel by the Captain of the Port of Long Island Sound. (2) The Captain...

  11. 77 FR 41271 - Safety Zone; Newburgh to Beacon Swim, Newburgh, Hudson River, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-13

    ... Acronyms DHS Department of Homeland Security FR Federal Register CFR Code of Federal Regulations NPRM... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Newburgh to Beacon Swim, Newburgh, Hudson... Newburgh, NY for the annual Newburgh Beacon Swim event. This temporary safety zone is necessary to...

  12. 77 FR 30451 - Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-23

    ... Management, U.S. Coast Guard Sector Buffalo; telephone 716-843-9343, email SectorBuffaloMarineSafety@uscg.mil... Federal Register (73 FR 3316). Public Meeting We do not now plan to hold a public meeting. But you may... near Olcott, NY. The Captain of the Port Buffalo has determined that fireworks launched proximate...

  13. 77 FR 30443 - Safety Zone; Alexandria Bay Chamber of Commerce, St. Lawrence River, Alexandria Bay, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-23

    .... Coast Guard Sector Buffalo; telephone 716-843-9343, email SectorBuffaloMarineSafety@uscg.mil . If you..., issue of the Federal Register (73 FR 3316). Public Meeting We do not now plan to hold a public meeting... the St. Lawrence River near Alexandria Bay, NY. The Captain of the Port Buffalo has determined...

  14. 77 FR 39411 - Safety Zone; Village of Sackets Harbor, Lake Ontario, Sackets Harbor, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-03

    ... Sector Buffalo; telephone 716- 843-9343, email SectorBuffaloMarineSafety@uscg.mil . If you have questions... be held on Lake Ontario in Sackets Harbor, NY. The Captain of the Port Buffalo has determined that... Buffalo has determined that this temporary safety zone is necessary to ensure the safety of spectators...

  15. 77 FR 38492 - Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-28

    ... Guard Sector Buffalo; telephone 716- 843-9343, email SectorBuffaloMarineSafety@uscg.mil . If you have... Security FR Federal Register NPRM Notice of Proposed Rulemaking A. Regulatory History and Information On... Fireworks, Lake Ontario, Olcott, NY in the Federal Register (77 FR 30451). We received no letters...

  16. 77 FR 39406 - Safety Zone; Tom Graves Memorial Fireworks, Port Bay, Wolcott, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-03

    .... Coast Guard Sector Buffalo; telephone 716- 843-9343, email SectorBuffaloMarineSafety@uscg.mil . If you... of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking A. Regulatory History and... display will be held on Port Bay near Wolcott, NY. The Captain of the Port Buffalo has determined...

  17. 78 FR 27856 - Golden Nematode; Removal of Regulated Areas in Livingston and Steuben Counties, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-13

    ... Animal and Plant Health Inspection Service 7 CFR Part 301 Golden Nematode; Removal of Regulated Areas in Livingston and Steuben Counties, NY AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION... Shea, Acting Administrator, Animal and Plant Health Inspection Service. BILLING CODE 3410-34-P...

  18. 76 FR 8654 - Regulated Navigation Area; Hudson River South of the Troy Locks, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-15

    ... the Troy Locks, NY'' in the Federal Register (75 FR 76943). We received no comments on the proposed... Regulated Navigation Area (RNA) on the navigable waters of the Hudson River in New York, south of the Troy... RNAs. Historically ice has been an impediment to navigation during certain times of the year on...

  19. 76 FR 60733 - Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-30

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY AGENCY... the Smith Point Bridge, 6.1, across Narrow Bay, between Smith Point and Fire Island, New York. The.... SUPPLEMENTARY INFORMATION: The Smith Point Bridge, across Narrow Bay, mile 6.1, between Smith Point and...

  20. 78 FR 23845 - Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-23

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY AGENCY... the Smith Point Bridge, mile 6.1, across Narrow Bay, between Smith Point and Fire Island, New York. The deviation is necessary to facilitate the Smith Point Triathlon. This deviation allows the...

  1. 77 FR 834 - Noise Exposure Map Update for Albany International Airport, Albany, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-06

    ... Federal Aviation Administration Noise Exposure Map Update for Albany International Airport, Albany, NY... County Airport Authority (ACAA), for Albany International Airport, under the provisions of 49 U.S.C... Protection Specialist, Federal Aviation Administration, New York Airports District Office, 600 Old...

  2. 77 FR 43167 - Safety Zone; Electric Zoo Fireworks, East River, Randall's Island, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-24

    ... DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking COTP... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Electric Zoo Fireworks, East River, Randall... Zone; Electronic Zoo Fireworks, East River, Randall's Island, NY. (a) Regulated Area. The...

  3. 77 FR 60044 - Safety Zone; DeStefano Wedding Fireworks Display, Patchogue Bay, Patchogue, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-02

    ... Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking A. Regulatory History... Zone; DeStefano Wedding Fireworks Display, Patchogue Bay, Patchogue, NY in the Federal Register (77 FR... navigable waters during the event. Entering into, transiting through, remaining, anchoring or mooring...

  4. 77 FR 73648 - Town of Stuyvesant, NY; Albany Engineering Corporation; Notice of Availability of Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-11

    ... and the Federal Energy Regulatory Commission's regulations, 18 CFR Part 380 (Order No. 486, 52 FR... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Town of Stuyvesant, NY; Albany Engineering Corporation; Notice...

  5. 78 FR 13811 - Safety Zone; Underwater Escape Event, Seaport, East River, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-01

    ... Federal Register on November 9, 2011 (76 FR 69614). ] Table 1 1. Merlini Underwater Escape Launch site... SECURITY Coast Guard 33 CFR Part 165 Safety Zone; Underwater Escape Event, Seaport, East River, NY AGENCY... escape artist event and associated pyrotechnics display. During the enforcement period, no person...

  6. 77 FR 39638 - Safety Zone; Barbara Harder Wedding Fireworks, Lake Erie, Lake View, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ... DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed Rulemaking A... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Barbara Harder Wedding Fireworks, Lake Erie, Lake View, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard...

  7. 77 FR 33309 - Safety Zone; Race on the Lake, Onondaga Lake, Syracuse, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-06

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Race on the Lake, Onondaga Lake, Syracuse... temporary safety zone on Onondaga Lake, Syracuse, NY. This safety zone is intended to restrict vessels from a portion of Onondaga Lake during the Race on the Lake powerboat races. This temporary safety...

  8. 77 FR 42513 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-19

    ... National Park Service Notice of Inventory Completion: New York University College of Dentistry, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The New York University College... were removed from Fresno County, CA. This notice is published as part of the National Park...

  9. 76 FR 31233 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-31

    ...) entitled ``Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY'' in the Federal Register (76 FR... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn... underwater explosive hazards in Gravesend Bay. This action will restrict unauthorized persons and...

  10. 75 FR 38714 - Safety Zone; Macy's Fourth of July Fireworks Display, Hudson River, New York, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-06

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Macy's Fourth of July Fireworks Display... Fourth of July Fireworks Display. This temporary safety zone is necessary to protect spectators and...'s Fourth of July Fireworks Display, Hudson River, NY, New York (a) Regulated area. The...

  11. Contribution of Cultural Eutrophication to Marsh Loss in Jamaica Bay (NY)

    EPA Science Inventory

    Loss of salt marsh area in the Jamaica Bay Estuary (NY) has accelerated in recent years, with loss rates as high as 45 acres per year. A contributing factor to this acceleration is likely cultural eutrophication due to over 6 decades of sewage effluent inputs. We examined marsh...

  12. ATMOSPHERIC MERCURY BEHAVIOR AT DIFFERENT ALTITUDES AT NY-ALESUND DURING SPRING 2003

    EPA Science Inventory

    Intensive field measurements of atmospheric mercury and related species were carried out in Ny Alesund, Spitsbergen during the spring of 2003 at two altitudes. Measurements were made at the Italian research station Dirigibile Italia (12m a.s.l.) and on the top of Zeppelin Mountai...

  13. 75 FR 31511 - Oneida Financial Corp., Oneida, NY; Approval of Conversion Application

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-03

    ... Office of Thrift Supervision Oneida Financial Corp., Oneida, NY; Approval of Conversion Application... Oneida Financial, MHC and Oneida Savings Bank, Oneida, New York, to convert to the stock form of... 20552, and the OTS Northeast Regional Office, Harborside Financial Center Plaza Five, Suite 1600,...

  14. 77 FR 19698 - Notice of Intent to Repatriate Cultural Items: Rochester Museum & Science Center, Rochester, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-02

    ... Seneca Nation of New York from a variety of sources. All of these medicine faces are currently in the... Trading Post, in Collins, NY. Traditional religious leaders of the Seneca Nation of New York have... present-day adherents. In the course of consultations with representatives of the Seneca Nation of...

  15. POLLUTION PREVENTION OPPORTUNITY ASSESSMENT: GENERAL MAIL AND VEHICLE MAINTENANCE FACILITY, UNITED STATES POSTAL SERVICE, BUFFALO, NY

    EPA Science Inventory

    The Pollution Prevention Opportunity Assessment (PPOA) summarized here was conducted at a U.S.Postal Service (USPS) Facility in Buffalo, NY. The PPOA documented and quantified waste generation at the General Mail Facility (GMF) where mail is processed, and at the Vehicle Maintena...

  16. 33 CFR 165.170 - Safety Zone: Triathlon, Ulster Landing, Hudson River, NY.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 CFR 165.23 apply. (2) No vessels will be allowed to transit the safety zone without the permission... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Safety Zone: Triathlon, Ulster... § 165.170 Safety Zone: Triathlon, Ulster Landing, Hudson River, NY. (a) Regulated area. The...

  17. 33 CFR 165.170 - Safety Zone: Triathlon, Ulster Landing, Hudson River, NY.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 CFR 165.23 apply. (2) No vessels will be allowed to transit the safety zone without the permission... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Safety Zone: Triathlon, Ulster... § 165.170 Safety Zone: Triathlon, Ulster Landing, Hudson River, NY. (a) Regulated area. The...

  18. 77 FR 41048 - Safety Zone; Hudson Valley Triathlon, Ulster Landing, Hudson River, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-12

    ...) 366-9826. SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal... Federal Register (76 FR 139) for this event. The Coast Guard is issuing this final rule without prior... Landing, NY for the 16th Annual Hudson Valley Triathlon swim event. This temporary safety zone...

  19. The tubulin-bound conformation of paclitaxel: T-taxol vs "PTX-NY".

    PubMed

    Yang, Yutao; Alcaraz, Ana A; Snyder, James P

    2009-03-27

    Nearly 35 years after its discovery and 11 years after FDA approval of paclitaxel (PTX) as a breakthrough anticancer drug, the 3-D structure of the agent bound to its beta-tubulin target was proposed to be T-Taxol. The latter bioactive form has recently been challenged by the Ojima group with a structure, "PTX-NY" ("REDOR Taxol"), in which the C-13 side chain is proposed to adopt a different conformation and an alternative hydrogen-bonding pattern in the tubulin binding site. Previously, the two conformers were compared to show that only T-Taxol fits the PTX-derived electron crystallographic density. That work has been extended by molecular mechanics and quantum chemical methods to reveal that the PTX-NY conformation is relatively less stable, on average, by 10-11 kcal/mol. In agreement with NMR studies, an 11 ns molecular dynamics treatment for PTX in an explicit water pool locates T-Taxol along the trajectory, but not PTX-NY. Docking of various PTX conformers into the electron crystallographic binding site of tubulin demonstrates that PTX-NY cannot be accommodated unless the pocket is reorganized in violation of the experimental constraints. Finally, analysis of the structures of T-Taxol and PTX-NY for their capacity to predict the existence of superpotent PTX analogues discloses that only the former forecasts such analogues, as now established by the T-Taxol-inspired synthesis of bridged taxanes. In sum, all empirical criteria support T-Taxol as the bound conformation of PTX on beta-tubulin in microtubules.

  20. Polymeric structure and host Toll-like receptor 4 dictate immunogenicity of NY-ESO-1 antigen in vivo.

    PubMed

    Liu, Yanan; Tian, Xiaoli; Leitner, Wolfgang W; Aldridge, Michael E; Zheng, Junying; Yu, Zhiya; Restifo, Nicholas P; Weiss, Richard; Scheiblhofer, Sandra; Xie, Chong; Sun, Ren; Cheng, Genhong; Zeng, Gang

    2011-10-28

    In search of intrinsic factors that contribute to the distinctively strong immunogenicity of a non-mutated cancer/testis antigen, we found that NY-ESO-1 forms polymeric structures through disulfide bonds. NY-ESO-1 binding to immature dendritic cells was dependent on its polymeric structure and involved Toll-like receptor-4 (TLR4) on the surface of immature dendritic cells in mouse and human. Gene gun-delivered plasmid encoding the wild-type NY-ESO-1 readily induced T cell-dependent antibody (Ab) responses in wild-type C57BL/10 mice but not TLR4-knock-out C57BL/10ScNJ mice. Disrupting polymeric structures of NY-ESO-1 by cysteine-to-serine (Cys-to-Ser) substitutions lead to diminished immunogenicity and altered TLR4-dependence in the induced Ab response. To demonstrate its adjuvant effect, NY-ESO-1 was fused with a major mugwort pollen allergen Art v 1 and a tumor-associated antigen, carbonic anhydrase 9. Plasmid DNA vaccines encoding the fusion genes generated robust immune responses against otherwise non-immunogenic targets in mice. Polymeric structure and TLR4 may play important roles in rendering NY-ESO-1 immunogenic and thus serve as a potent molecular adjuvant. NY-ESO-1 thus represents the first example of a cancer/testis antigen that is a also damage-associated molecular pattern.

  1. Effector memory and central memory NY-ESO-1-specific re-directed T cells for treatment of multiple myeloma.

    PubMed

    Schuberth, P C; Jakka, G; Jensen, S M; Wadle, A; Gautschi, F; Haley, D; Haile, S; Mischo, A; Held, G; Thiel, M; Tinguely, M; Bifulco, C B; Fox, B A; Renner, C; Petrausch, U

    2013-04-01

    The cancer-testis antigen NY-ESO-1 is a potential target antigen for immune therapy expressed in a subset of patients with multiple myeloma. We generated chimeric antigen receptors (CARs) recognizing the immunodominant NY-ESO-1 peptide 157-165 in the context of HLA-A*02:01 to re-direct autologous CD8(+) T cells towards NY-ESO-1(+) myeloma cells. These re-directed T cells specifically lysed NY-ESO-1(157-165)/HLA-A*02:01-positive cells and secreted IFNγ. A total of 40% of CCR7(-) re-directed T cells had an effector memory phenotype and 5% a central memory phenotype. Based on CCR7 cell sorting, effector and memory CAR-positive T cells were separated and CCR7(+) memory cells demonstrated after antigen-specific re-stimulation downregulation of CCR7 as sign of differentiation towards effector cells accompanied by an increased secretion of memory signature cytokines such as IL-2. To evaluate NY-ESO-1 as potential target antigen, we screened 78 bone marrow biopsies of multiple myeloma patients where NY-ESO-1 protein was found to be expressed by immunohistochemistry in 9.7% of samples. Adoptively transferred NY-ESO-1-specific re-directed T cells protected mice against challenge with endogenously NY-ESO-1-positive myeloma cells in a xenograft model. In conclusion, re-directed effector- and central memory T cells specifically recognized NY-ESO-1(157-165)/ HLA-A*02:01-positive cells resulting in antigen-specific functionality in vitro and in vivo.

  2. Enhancing whole-tumor cell vaccination by engaging innate immune system through NY-ESO-1/dendritic cell interactions.

    PubMed

    Xu, Le; Zheng, Junying; Nguyen, David H; Luong, Quang T; Zeng, Gang

    2013-10-01

    NY-ESO-1 is a cancer/germline antigen (Ag) with distinctively strong immunogenicity. We have previously demonstrated that NY-ESO-1 serves as an endogenous adjuvant by engaging dendritic cell (DC)-surface receptors of calreticulin (CRT) and toll-like receptor (TLR) 4. In the present study, NY-ESO-1 was investigated for its immunomodulatory roles as a molecular adjuvant in whole-tumor cell vaccines using the Renca kidney cancer model. Renca cells were genetically engineered to express NY-ESO-1 on the cell surface to enhance direct interactions with DC. The effect of ectopic cell-surface expression of NY-ESO-1 was investigated on tumor immunogenicity, DC activation, cytotoxic T lymphocytes against model tumor-associated Ags, and the effectiveness of the modified tumor cells as a therapeutic whole-cell vaccine. Cell-surface expression of NY-ESO-1 was able to reduce the tumor growth of Renca cells in BALB/c mice, although the modification did not alter cell proliferation rate in vitro. Directly engaging the innate immune system through NY-ESO-1 facilitated the interaction of tumor cells with DC, leading to enhanced DC activation and subsequent tumor-specific T-cell priming. When used as a therapeutic whole-cell vaccine, Renca cells with NY-ESO-1 on the surface mediated stronger inhibitory effects on tumor growth and metastasis compared with parental Renca or Renca cells expressing a control protein GFP on the surface. Augmented antitumor efficacy correlated with increased CD8 T-cell infiltration into tumors and decreased myeloid-derived suppressor cells and regulatory T cells in the spleen. As a cancer/germline Ag and as an immunomodulatory adjuvant through engaging innate immune receptors, NY-ESO-1 offers a unique opportunity for improved whole-tumor cell vaccinations upon the classic GM-CSF-engineered cell vaccines.

  3. MAGE-A3/4 and NY-ESO-1 antigens expression in metastatic esophageal squamous cell carcinoma.

    PubMed

    Bujas, T; Marusic, Z; Peric Balja, M; Mijic, A; Kruslin, B; Tomas, D

    2011-03-21

    In the present study we analyzed immunohistochemical expression of MAGE-A 3/4 and NY-ESO-1 in 55 samples of esophageal squamous cell carcinomas (ESCC) and their respective lymph node metastases. To our knowledge this is the first study to assess and compare the expression of these antigens in ESCC lymph node metastases. Fifty (90.9%) primary ESCC were positive for MAGE-A 3/4 and 53 (96.6%) were positive for NY-ESO-1. MAGE-A 3/4 was expressed in all lymph node metastases and the intensity of expression was high in a majority of cases. NY-ESO-1 was negative in 2 (7.1%) lymph nodes metastases, while the reaction was predominantly moderate in the positive group. In primary tumors MAGE-A 3/4 showed a significantly higher intensity of expression compared to NY-ESO-1 (P=0.047), while in lymph node metastases the intensity of expression was not significantly different (P=0.387). Primary tumors with and without lymph node metastases showed no significant differences in MAGE-A 3/4 (P=0.672) and NY-ESO-1 (P=0.444) expression. Intensity of MAGE-A 3/4 (P=0.461) and NY-ESO-1 (P=0.414) expression in primary tumors was not significantly different compared to the expression in their respective lymph nodes metastases. Expression of MAGE-A 3/4 in primary tumors showed significant positive correlation with primary tumor expression of NY-ESO-1 (P=0.021) but no significant correlation with the expression of MAGE-A 3/4 in lymph node metastases (P=0.056). Expression of NY-ESO-1 in primary tumors showed significant positive correlation with the expression of NY-ESO-1 in lymph node metastases (P=0.001) and significant negative correlation with patients’ age (P<0.001). Expression of MAGE-A 3/4 and NY-ESO-1 in primary tumors and lymph node metastases showed no significant correlation with prognostic parameters such as tumor grade and TNM stage (P>0.05). We have shown different levels of MAGE-A 3/4 and NY-ESO-1 expression in almost all specimens of primary tumor and lymph node metastases

  4. NY-ESO-1 antigen-reactive T cell receptors exhibit diverse therapeutic capability.

    PubMed

    Sommermeyer, Daniel; Conrad, Heinke; Krönig, Holger; Gelfort, Haike; Bernhard, Helga; Uckert, Wolfgang

    2013-03-15

    The cancer-testis antigen NY-ESO-1 has been used as a target for different immunotherapies like vaccinations and adoptive transfer of antigen-specific cytotoxic T cells, as it is expressed in various tumor types and has limited expression in normal cells. The in vitro generation of T cells with defined antigen specificity by T cell receptor (TCR) gene transfer is an established method to create cells for immunotherapy. However, an extensive characterization of TCR which are candidates for treatment of patients is crucial for successful therapies. The TCR has to be efficiently expressed, their affinity to the desired antigen should be high enough to recognize low amounts of endogenously processed peptides on tumor cells, and the TCR should not be cross-reactive to other antigens. We characterized three NY-ESO-1 antigen-reactive cytotoxic T lymphocyte clones which were generated by different approaches of T cell priming (autologous, allogeneic), and transferred their TCR into donor T cells for more extensive evaluations. Although one TCR most efficiently bound MHC-multimers loaded with NY-ESO-1 peptide, T cells expressing this transgenic TCR were not able to recognize endogenously processed antigen. A second TCR recognized HLA-A2 independent of the bound peptide beside its much stronger recognition of NY-ESO-1 bound to HLA-A2. A third TCR displayed an intermediate but peptide-specific performance in all functional assays and, therefore, is the most promising candidate TCR for further clinical development. Our data indicate that multiple parameters of TCR gene-modified T cells have to be evaluated to identify an optimal TCR candidate for adoptive therapy.

  5. A novel murine T-cell receptor targeting NY-ESO-1.

    PubMed

    Rosati, Shannon F; Parkhurst, Maria R; Hong, Young; Zheng, Zhili; Feldman, Steven A; Rao, Mahadev; Abate-Daga, Daniel; Beard, Rachel E; Xu, Hui; Black, Mary A; Robbins, Paul F; Schrump, David A; Rosenberg, Steven A; Morgan, Richard A

    2014-04-01

    Cancer testis antigens, such as NY-ESO-1, are expressed in a variety of prevalent tumors and represent potential targets for T-cell receptor (TCR) gene therapy. DNA encoding a murine anti-NY-ESO-1 TCR gene (mTCR) was isolated from immunized HLA-A*0201 transgenic mice and inserted into a γ-retroviral vector. Two mTCR vectors were produced and used to transduce human PBL. Transduced cells were cocultured with tumor target cell lines and T2 cells pulsed with the NY-ESO-1 peptide, and assayed for cytokine release and cell lysis activity. The most active TCR construct was selected for production of a master cell bank for clinical use. mTCR-transduced PBL maintained TCR expression in short-term and long-term culture, ranging from 50% to 90% efficiency 7-11 days after stimulation and 46%-82% 10-20 days after restimulation. High levels of interferon-γ secretion were observed (1000-12000 pg/mL), in tumor coculture assays and recognition of peptide-pulsed cells was observed at 0.1 ng/mL, suggesting that the new mTCR had high avidity for antigen recognition. mTCR-transduced T cells also specifically lysed human tumor targets. In all assays, the mTCR was equivalent or better than the comparable human TCR. As the functional activity of TCR-transduced cells may be affected by the formation of mixed dimers, mTCRs, which are less likely to form mixed dimers with endogenous hTCRs, may be more effective in vivo. This new mTCR targeted to NY-ESO-1 represents a novel potential therapeutic option for adoptive cell-transfer therapy for a variety of malignancies.

  6. Kate Bárány: a life of science, teaching, and service.

    PubMed

    Solaro, R John; Rao, Mrinalini C

    2012-12-01

    We celebrate the lives of Michael and Kate Bárány in this issue of the Journal of Muscle Research and Cell Motility. Kate and Michael died within weeks of each other in 2011. Joe Chalovich has written about Michael and we write about Kate. As emphasized by Joe, Kate, and Michael were remarkable individuals who survived the Holocaust, the Hungarian revolution, and emerged from as much adversity as one might imagine to become productive scientists, educators, citizens, and symbols of the durability of the human spirit. They present their own story in an essay (Bárány and Bárány 2000) published in a monograph "Selected Topics in the History of Biochemistry." Rather than repeating much of the list of scientific achievements chronicled in these papers, we focus here on Kate, especially in her role as an individual and partner in science, while at the same time being an accomplished teacher, and a champion of women in science.

  7. A cancer vaccine induces expansion of NY-ESO-1-specific regulatory T cells in patients with advanced melanoma.

    PubMed

    Ebert, Lisa M; MacRaild, Sarah E; Zanker, Damien; Davis, Ian D; Cebon, Jonathan; Chen, Weisan

    2012-01-01

    Cancer vaccines are designed to expand tumor antigen-specific T cells with effector function. However, they may also inadvertently expand regulatory T cells (Treg), which could seriously hamper clinical efficacy. To address this possibility, we developed a novel assay to detect antigen-specific Treg based on down-regulation of surface CD3 following TCR engagement, and used this approach to screen for Treg specific to the NY-ESO-1 tumor antigen in melanoma patients treated with the NY-ESO-1/ISCOMATRIX™ cancer vaccine. All patients tested had Treg (CD25(bright) FoxP3(+) CD127(neg)) specific for at least one NY-ESO-1 epitope in the blood. Strikingly, comparison with pre-treatment samples revealed that many of these responses were induced or boosted by vaccination. The most frequently detected response was toward the HLA-DP4-restricted NY-ESO-1(157-170) epitope, which is also recognized by effector T cells. Notably, functional Treg specific for an HLA-DR-restricted epitope within the NY-ESO-1(115-132) peptide were also identified at high frequency in tumor tissue, suggesting that NY-ESO-1-specific Treg may suppress local anti-tumor immune responses. Together, our data provide compelling evidence for the ability of a cancer vaccine to expand tumor antigen-specific Treg in the setting of advanced cancer, a finding which should be given serious consideration in the design of future cancer vaccine clinical trials.

  8. Immune escape from NY-ESO-1-specific T-cell therapy via loss of heterozygosity in the MHC.

    PubMed

    Klippel, Z K; Chou, J; Towlerton, A M; Voong, L N; Robbins, P; Bensinger, W I; Warren, E H

    2014-03-01

    Adoptive immunotherapy of tumors with T cells specific for the cancer-testis antigen NY-ESO-1 has shown great promise in preclinical models and in early stage clinical trials. Tumor persistence or recurrence after NY-ESO-1-specific therapy occurs, however, and the mechanisms of recurrence remain poorly defined. In a murine xenograft model of NY-ESO-1(+) multiple myeloma, we observed tumor recurrence after adoptive transfer of CD8(+) T cells genetically redirected to the prototypic NY-ESO-1157-165 peptide presented by HLA-A*02:01. Analysis of the myeloma cells that had escaped from T-cell control revealed intact expression of NY-ESO-1 and B2M, but selective, complete loss of HLA-A*02:01 expression from the cell surface. Loss of heterozygosity (LOH) in the major histocompatibility complex (MHC) involving the HLA-A locus was identified in the tumor cells, and further analysis revealed selective loss of the allele encoding HLA-A*02:01. Although LOH involving the MHC has not been described in myeloma patients with persistent or recurrent disease after immune therapies such as allogeneic hematopoietic cell transplantation (HCT), it has been described in patients with acute myelogenous leukemia who relapsed after allogeneic HCT. These results suggest that MHC loss should be evaluated in patients with myeloma and other cancers who relapse after adoptive NY-ESO-1-specific T-cell therapy.

  9. Checkpoint Antibodies but not T Cell-Recruiting Diabodies Effectively Synergize with TIL-Inducing γ-Irradiation.

    PubMed

    Hettich, Michael; Lahoti, Jayashree; Prasad, Shruthi; Niedermann, Gabriele

    2016-08-15

    T cell-recruiting bispecific antibodies (bsAb) show promise in hematologic malignancies and are also being evaluated in solid tumors. In this study, we investigated whether T cell-recruiting bsAbs synergize with hypofractionated tumor radiotherapy (hRT) and/or blockade of the programmed death-1 (PD-1) immune checkpoint, both of which can increase tumor-infiltrating lymphocyte (TIL) numbers. Unexpectedly, large melanomas treated with hRT plus bsAb (AC133×CD3) relapsed faster than those treated with hRT alone, accompanied by massive TIL apoptosis. This fast relapse was delayed by the further addition of anti-PD-1. Mechanistic investigations revealed restimulation-induced cell death mediated by BIM and FAS as an additional cause of bsAb-mediated TIL depletion. In contrast, the double combination of hRT and anti-PD-1 strongly increased TIL numbers, and even very large tumors were completely eradicated. Our study reveals the risk that CD3-engaging bsAbs can induce apoptotic TIL depletion followed by rapid tumor regrowth, reminiscent of tolerance induction by CD3 mAb-mediated T-cell depletion, warranting caution in their use for the treatment of solid tumors. Our findings also argue that combining radiotherapy and anti-PD-1 can be quite potent, including against very large tumors. Cancer Res; 76(16); 4673-83. ©2016 AACR. PMID:27302161

  10. Induction of CD8 T-cell responses restricted to multiple HLA class I alleles in a cancer patient by immunization with a 20-mer NY-ESO-1f (NY-ESO-1 91-110) peptide.

    PubMed

    Eikawa, Shingo; Kakimi, Kazuhiro; Isobe, Midori; Kuzushima, Kiyotaka; Luescher, Immanuel; Ohue, Yoshihiro; Ikeuchi, Kazuhiro; Uenaka, Akiko; Nishikawa, Hiroyoshi; Udono, Heiichiro; Oka, Mikio; Nakayama, Eiichi

    2013-01-15

    Immunogenicity of a long 20-mer NY-ESO-1f peptide vaccine was evaluated in a lung cancer patient TK-f01, immunized with the peptide with Picibanil OK-432 and Montanide ISA-51. We showed that internalization of the peptide was necessary to present CD8 T-cell epitopes on APC, contrasting with the direct presentation of the short epitope. CD8 T-cell responses restricted to all five HLA class I alleles were induced in the patient after the peptide vaccination. Clonal analysis showed that B*35:01 and B*52:01-restricted CD8 T-cell responses were the two dominant responses. The minimal epitopes recognized by A*24:02, B*35:01, B*52:01 and C*12:02-restricted CD8 T-cell clones were defined and peptide/HLA tetramers were produced. NY-ESO-1 91-101 on A*24:02, NY-ESO-1 92-102 on B*35:01, NY-ESO-1 96-104 on B*52:01 and NY-ESO-1 96-104 on C*12:02 were new epitopes first defined in this study. Identification of the A*24:02 epitope is highly relevant for studying the Japanese population because of its high expression frequency (60%). High affinity CD8 T-cells recognizing tumor cells naturally expressing the epitopes and matched HLA were induced at a significant level. The findings suggest the usefulness of a long 20-mer NY-ESO-1f peptide harboring multiple CD8 T-cell epitopes as an NY-ESO-1 vaccine. Characterization of CD8 T-cell responses in immunomonitoring using peptide/HLA tetramers revealed that multiple CD8 T-cell responses comprised the dominant response.

  11. On the performance of multirate filterbanks: Quantification of shift variance and cyclostationarity in the works of Til Aach

    NASA Astrophysics Data System (ADS)

    Bregovic, Robert; Gotchev, Atanas

    2014-02-01

    The paper discusses the issues of shift variance and cyclostationarity in multirate filterbanks as investigated in a series of articles by Til Aach. In its first part, the paper overviews the most important properties of multirate filterbanks such as perfect reconstruction, sampling rate conversion factors, number and type of subbands and subdivisions, orthogonality and bio-orthogonality, and frequency selectivity and preservation of polynomials. This part in intended introduce the reader to the topic and make a bridge to the properties of shift variance and cyclostationarity discussed next. Criteria for shift (in)variance and cyclostationarity as derived by Til Aach are presented and commented and conclusions about their importance are made.

  12. Combination of autoantibodies against NY-ESO-1 and viral capsid antigen immunoglobulin A for improved detection of nasopharyngeal carcinoma.

    PubMed

    Peng, Yu-Hui; Xu, Yi-Wei; Qiu, Si-Qi; Hong, Chao-Qun; Zhai, Tian-Tian; Li, En-Min; Xu, Li-Yan

    2014-09-01

    Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in Southern China and Southeast Asia, and early detection remains a challenge. Autoantibodies have been found to precede the manifestations of symptomatic cancer by several months to years, making their identification of particular relevance for early detection. In the present study, the diagnostic value of serum autoantibodies against NY-ESO-1 in NPC patients was evaluated. The study included 112 patients with NPC and 138 normal controls. Serum levels of autoantibodies against NY-ESO-1 and classical Epstein-Barr virus marker, viral capsid antigen immunoglobulin A (VCA-IgA), were measured by enzyme-linked immunosorbent assay. Measurement of autoantibodies against NY-ESO-1 and VCA-IgA demonstrated a sensitivity/specificity of 42.9/94.9% [95% confidence interval (CI), 33.7-52.6/89.4-97.8%] and 55.4/95.7% (95% CI, 45.7-64.7/90.4-98.2%), respectively. The area under receiver operating characteristic curve for autoantibodies against NY-ESO-1 (0.821; 95% CI, 0.771-0.871) was marginally lower than that for VCA-IgA (0.860; 95% CI, 0.810-0.910) in NPC. The combination of autoantibodies against NY-ESO-1 and VCA-IgA yielded an enhanced sensitivity of 80.4% (95% CI, 71.6-87.0%) and a specificity of 90.6% (95% CI, 84.1-94.7%). Moreover, detection of autoantibodies against NY-ESO-1 could differentiate early-stage NPC patients from normal controls. Our results suggest that autoantibodies against NY-ESO-1 may serve as a potential biomarker, as a supplement to VCA-IgA, for the screening and diagnosis of NPC.

  13. Measurement of serum antibodies against NY-ESO-1 by ELISA: A guide for the treatment of specific immunotherapy for patients with advanced colorectal cancer.

    PubMed

    Long, Yan-Yan; Wang, Yu; Huang, Qian-Rong; Zheng, Guang-Shun; Jiao, Shun-Chang

    2014-10-01

    NY-ESO-1 has been identified as one of the most immunogenic antigens; thus, is a highly attractive target for cancer immunotherapy. The present study analyzed the expression of serum antibodies (Abs) against NY-ESO-1 in patients with advanced colorectal cancer (CRC), with the aim of guiding the treatment of NY-ESO-1-based specific-immunotherapy for these patients. Furthermore, the present study was the first to evaluate the kinetic expression of anti-NY-ESO-1 Abs and investigate the possible influencing factors. A total of 239 serum samples from 155 pathologically confirmed patients with advanced CRC (stages III and IV) were collected. The presence of spontaneous Abs against NY-ESO-1 was analyzed using an enzyme-linked immunosorbent assay (ELISA). The results demonstrated that 24.5% (38/155) of the investigated patients were positive for NY-ESO-1-specific Abs. No statistically significant correlations were identified between the expression of anti-NY-ESO-1 Abs and clinicopathological parameters, including age and gender, location, grading, local infiltration, lymph node status, metastatic status and K-ras mutation status (P>0.05). In 59 patients, the kinetic expression of anti-NY-ESO-1 Abs was analyzed, of which 14 patients were initially positive and 45 patients were initially negative. Notably, 16/59 (27.1%) patients changed their expression status during the study period, and the initially positive patients were more likely to change compared with the initially negative patients (85.7 vs. 8.8%; P<0.001). Therefore, monitoring serum Abs against NY-ESO-1 by ELISA is an easy and feasible method. The high expression rate of NY-ESO-1-specific Abs in CRC patients indicates that measuring the levels of serum Abs against NY-ESO-1 may guide the treatment of NY-ESO-1-based specific immunotherapy for patients with advanced CRC.

  14. The pulsating sdB+M eclipsing system NY Virginis and its circumbinary planets

    NASA Astrophysics Data System (ADS)

    Lee, Jae Woo; Hinse, Tobias Cornelius; Youn, Jae-Hyuck; Han, Wonyong

    2014-12-01

    We searched for circumbinary planets orbiting NY Vir in historical eclipse times including our long-term CCD data. 68 times of minimum light with accuracies better than 10 s were used for the ephemeris computations. The best fit to those timings indicated that the orbital period of NY Vir has varied due to a combination of two sinusoids with periods of P3 = 8.2 yr and P4 = 27.0 yr and semi-amplitudes of K3 = 6.9 s and K4 = 27.3 s, respectively. The periodic variations most likely arise from a pair of light-time effects due to the presence of third and fourth bodies that are gravitationally bound to the eclipsing pair. We have derived the orbital parameters and the minimum masses, M3sin i3 = 2.8 MJup and M4sin i4 = 4.5 MJup, of both objects. A dynamical analysis suggests that the outer companion is less likely to orbit the binary on a circular orbit. Instead, we show that future timing data might push its eccentricity to moderate values for which the system exhibits long-term stability. The results demonstrate that NY Vir is probably a star-planet system, which consists of a very close binary star and two giant planets. The period ratio P3/P4 suggests that a long-term gravitational interaction between them would result in capture into a nearly 3:10 mean motion resonance. When the presence of the circumbinary planets is verified and understood more comprehensively, the formation and evolution of this planetary system should be advanced greatly.

  15. Novel rhamnolipid biosurfactants produced by a polycyclic aromatic hydrocarbon-degrading bacterium Pseudomonas aeruginosa strain NY3

    PubMed Central

    Nie, Maiqian; Yin, Xihou; Ren, Chunyan; Wang, Yang; Xu, Feng; Shen, Qirong

    2014-01-01

    A novel rhamnolipid biosurfactant-producing and Polycyclic Aromatic Hydrocarbon (PAH)-degrading bacterium Pseudomonas aeruginosa strain NY3 was isolated from petroleum-contaminated soil samples. Strain NY3 was characterized by its extraordinary capacity to produce structurally diverse rhamnolipids. A total of 25 rhamnolipid components and 37 different parent molecular ions, representing various metal ion adducts (Na+, 2Na+ and K+), were detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among these compounds are ten new rhamnolipids. In addition to its biosurfactant production, strain NY3 was shown to be capable of efficient degradation of PAHs as well as synergistic improvement in the degradation of high molecular weight PAHs by its biosurfactant. These findings have added novel members to the rhamnolipid group and expanded current knowledge regarding the diversity and productive capability of rhamnolipid biosurfactants from a single specific strain with variation of only one carbon source. Additionally, this paper lays the foundation for improvement in the yield of NY3BS and study of the degradation pathway(s) of PAHs in P. aeruginosa strain NY3. PMID:20580808

  16. Isolation of a halophilic bacterium, Bacillus sp. strain NY-6 for organic contaminants removal in saline wastewater on ship

    NASA Astrophysics Data System (ADS)

    Gao, Jie; Yu, Zhenjiang; Zhang, Xiaohui; Zhao, Dan; Zhao, Fangbo

    2013-06-01

    The objective of this research was to examine if certain strains of Bacillus bacteria, could survive in dry powder products and if so, could the bacteria degrade organic contaminants in saline wastewater on a ship. As part of the study, we isolated 7 domesticated strains named NY1, NY2,..., and NY7, the strain NY6 showed to have the best performance for organic matter degradation and could survive in dry powder more than 3 months. NY6 was identified as Bacillus aerius, based on the morphological and physic-chemical properties. Its optimal growth conditions were as follows: salinity was 2%; temperature was 37°C; pH was in 6.5-7.0; best ratio of C: N: P was 100:5:1. The capability of its dry powder for Chemical Oxygen Demand (COD) removal was 800mg COD/g in synthesized marine wastewater with 2% salinity. The spores in the dry powder were 1.972×108 g -1.

  17. Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells.

    PubMed

    Mobergslien, Anne; Vasovic, Vlada; Mathiesen, Geir; Fredriksen, Lasse; Westby, Phuong; Eijsink, Vincent G H; Peng, Qian; Sioud, Mouldy

    2015-01-01

    Given their safe use in humans and inherent adjuvanticity, Lactic Acid Bacteria may offer several advantages over other mucosal delivery strategies for cancer vaccines. The objective of this study is to evaluate the immune responses in mice after oral immunization with Lactobacillus (L) plantarum WCFS1 expressing a cell-wall anchored tumor antigen NY-ESO-1. And to investigate the immunostimulatory potency of this new candidate vaccine on human dendritic cells (DCs). L. plantarum displaying NY-ESO-1 induced NY-ESO-1 specific antibodies and T-cell responses in mice. By contrast, L. plantarum displaying conserved proteins such as heat shock protein-27 and galectin-1, did not induce immunity, suggesting that immune tolerance to self-proteins cannot be broken by oral administration of L. plantarum. With respect to immunomodulation, immature DCs incubated with wild type or L. plantarum-NY-ESO-1 upregulated the expression of co-stimulatory molecules and secreted a large amount of interleukin (IL)-12, TNF-α, but not IL-4. Moreover, they upregulated the expression of immunosuppressive factors such as IL-10 and indoleamine 2,3-dioxygenase. Although L. plantarum-matured DCs expressed inhibitory molecules, they stimulated allogeneic T cells in-vitro. Collectively, the data indicate that L. plantarum-NY-ESO-1 can evoke antigen-specific immunity upon oral administration and induce DC maturation, raising the potential of its use in cancer immunotherapies.

  18. Laboratory data in support of hydraulically fracturing EGSP NY well No. 3. Final report

    SciTech Connect

    Not Available

    1982-04-01

    By the results in this report, it appears that the Devonian shale formation in the EGSP NY No. 3 well maybe successfully hydraulically fractured using nitrogen foam as a fracturing fluid. However, overall success will be dependent on the degree of natural fracturing within the formation. Then, an induced fracture linking the areas of natural gas concentration to the wellbore can provide sufficient fracture conductivity for commercial production levels. If extensive fluid loss is experienced during treatment, irreparable damage to the fracture faces, both induced and natural, may limit the overall well productivity. 6 references, 6 figures, 5 tables.

  19. TiO2/GeOxNy stacked gate dielectrics for Ge-MOSFETs

    NASA Astrophysics Data System (ADS)

    Bera, M. K.; Mahata, C.; Chakraborty, A. K.; Nandi, S. K.; Tiwari, Jitendra N.; Hung, Jui-Yi; Maiti, C. K.

    2007-12-01

    In this work, we present the results of physical and electrical characterization of Ti-based high-k gate dielectrics on Ge substrates. Titanium tetrakis iso- propoxide (TTIP) was used as the organometallic source for the deposition of ultra-thin TiO2 films on p-Ge (1 0 0) at low temperature (<200 °C) by plasma enhanced chemical vapor deposition (PECVD) technique in a microwave (700 W, 2.45 GHz) plasma cavity discharge system at a pressure of ~65 Pa. The presence of an ultra-thin lossy GeO2 interfacial layer between the deposited high-k film and the substrate, results in frequency-dependent capacitance-voltage (C-V) characteristics in strong accumulation and a high interface state density (~1013 cm-2 eV-1). To improve the electrical properties, nitrogen engineering has been employed to convert the lossy GeO2 interfacial layer to its oxynitride, thus forming TiO2/GeOxNy/Ge stacked-gate structure with improved interface/electrical properties. Different N sources, such as NO, NH3 and NO/NH3, have been used for nitrogen engineering. XPS and Raman spectroscopy analyses have been used for surface morphological study. Electrical properties, such as gate leakage current density, interface state density, charge trapping, flatband voltage shift, etc, have been studied in detail for TiO2/GeOxNy/Ge MIS capacitors using the current-voltage (I-V), capacitance-voltage (C-V), conductance-voltage (G-V) and stress (both constant voltage and current) measurements. Although a significant improvement in electrical characteristics has been observed after nitridation in general, the formation of the interfacial GeOxNy layer, obtained from NO-plasma nitridation, is found to provide the maximum improvement among all the nitridation techniques used in this study. It is shown that the insertion of an ultra-thin oxynitride (GeOxNy) interfacial layer is advantageous for producing gate-quality TiO2 high-k dielectric stacks on Ge substrates.

  20. Nitrogen effects on crystallization kinetics of amorphous TiOxNy thin films

    SciTech Connect

    Hukari, Kyle; Dannenberg, Rand; Stach, E.A.

    2001-03-30

    The crystallization behavior of amorphous TiOxNy (x>>y) thin films was investigated by in-situ transmission electron microscopy. The Johnson-Mehl-Avrami-Kozolog (JMAK) theory is used to determine the Avrami exponent, activation energy, and the phase velocity pre-exponent. Addition of nitrogen inhibits diffusion, increasing the nucleation temperature, while decreasing the growth activation energy. Kinetic variables extracted from individual crystallites are compared to JMAK analysis of the fraction transformed and a change of 6 percent in the activation energy gives agreement between the methods. From diffraction patterns and index of refraction the crystallized phase was found to be predominantly anatase.

  1. Diabetes prevalence among Puerto Rican adults in New York City, NY, 2000.

    PubMed

    Melnik, Thomas A; Hosler, Akiko S; Sekhobo, Jackson P; Duffy, Thomas P; Tierney, Edward F; Engelgau, Michael M; Geiss, Linda S

    2004-03-01

    This study assessed the prevalence of diagnosed diabetes and associated characteristics among Puerto Rican adults in New York City, NY, with a random-digit-dialed telephone survey with a dual-frame sampling design. Overall, 11.3% (95% confidence interval = 8.7%, 14.0%) had diagnosed diabetes; diabetes was significantly related to age, obesity, and family history; and the prevalence was high among those with the least education. This study showed the ability to obtain critically needed diabetes information from ethnic minorities at the local level.

  2. Lidar temperature measurements at NY-Alesund (79N) during winter, 1998

    NASA Technical Reports Server (NTRS)

    McGee, T. J.; Gross, M.; Newman, P.; Beyerle, G.; Beninga, I.; Dahl, A.; Neuber, R.; Wahl, P.; Schrems, O.

    1998-01-01

    During January and February, 1998, a measurements campaign was held at the Network for the Detection of Stratospheric Change (NDSC) Arctic site at Ny-Alesund (78.9N). Lidar measurements of ozone, temperature and aerosol parameters were made along with balloon sonde and microwave measurements of ozone. Atmospheric temperatures were measured between 10 and 70 km. During the time of the campaign an strong warming occurred at the stratopause, elevating the measured temperature by as much as 80 K. The height of the stratopause descended at this time to below 40 km.

  3. The Ny-Alesund aerosol and ozone measurements intercomparison campaign 1997/1998 (NAOMI-1998)

    NASA Technical Reports Server (NTRS)

    Neuber, R.; Beyerle, G.; Beninga, I.; VonderGathen, P.; Rairoux, P.; Schrems, O.; Wahl, P.; Gross, M.; McGee, Th.; Iwasaka, Y.; Fujiwara, M.; Shibata, T.; Klein, U.; Steinbrecht, W.

    1998-01-01

    An intercomparison campaign for Lidar measurements of stratospheric ozone and aerosol has been conducted at the Primary Station of the Network for the Detection of Stratospheric Change (NDSC) in Ny-Alesund/Spitsbergen during January-February 1998. In addition to local instrumentation, the NDSC mobile ozone lidar from NASA/GSFC and the mobile aerosol lidar from Alfred Wegener Institute (AWI) participated. The aim is the validation of stratospheric ozone and aerosol profile measurements according to NDSC guidelines. This paper briefly presents the employed instruments and outlines the campaign. Results of the blind intercomparison of ozone profiles are given in a companion paper and temperature measurements are described in this issue.

  4. Expression of the cancer/testis antigen NY-ESO-1 in primary and metastatic malignant melanoma (MM) - correlation with prognostic factors

    PubMed Central

    Velazquez, Elsa F.; Jungbluth, Achim A.; Yancovitz, Molly; Gnjatic, Sacha; Adams, Sylvia; O'Neill, David; Zavilevich, Kira; Albukh, Tatyana; Christos, Paul; Mazumdar, Madhu; Pavlick, Anna; Polsky, David; Shapiro, Richard; Berman, Russell; Spira, Joanna; Busam, Klaus; Osman, Iman

    2007-01-01

    Cancer/testis (CT) antigens are potential targets for cancer immunotherapy, with NY-ESO-1 being among the most immunogenic. In several clinical trials in malignant melanoma (MM) patients, NY-ESO-1 protein/peptides showed clear evidence of inducing specific immunity. However, little is known about NY-ESO-1 expression in primary and metastatic MM and its relationship to disease progression. We analyzed NY-ESO-1 expression immunohistochemically in a series of primary and metastatic MMs and its relation to prognostic parameters and survival. We studied 61 primary and 63 metastatic MM specimens (from 61 and 56 patients, respectively). The prevalence of NY-ESO-1 expression was significantly higher in metastatic versus primary tumors [18/56 (32%) versus 8/61 (13%), P = 0.015]. There was a significant association between initial stage at presentation and NY-ESO-1 expression [stage I (3.45%), stage II (9.52%) and stage III (45.45%), P = 0.0014]. Primary MMs expressing NY-ESO-1 were significantly thicker than NY-ESO-1 negative cases (median thickness 4.7 mm versus 1.53 mm respectively, P = 0.03). No significant difference was seen in overall survival. In conclusion, NY-ESO-1 is more frequently expressed in metastatic than in primary MM and its expression is associated with thicker primary lesions and a higher frequency of metastatic disease, indicative of a worse prognosis. Our study suggests that patients with metastatic MM who express NY-ESO-1 may benefit from NY-ESO-1-based immunotherapy. PMID:17625806

  5. Evaluation of LAGE-1 and NY-ESO-1 expression in multiple myeloma patients to explore possible benefits of their homology for immunotherapy.

    PubMed

    de Carvalho, Fabricio; Vettore, Andre L; Inaoka, Riguel J; Karia, Bruno; Andrade, Valéria C C; Gnjatic, Sacha; Jungbluth, Achim A; Colleoni, Gisele W B

    2011-01-20

    Due to the high homology between the LAGE-1 and NY-ESO-1 proteins, we hypothesized that an anti-NY-ESO-1 vaccine might elicit LAGE-1 immunity and hence may be effective in multiple myeloma (MM) patients with LAGE-1-positive/NY-ESO-1-negative tumors. Therefore, we set out to evaluate LAGE-1 and NY-ESO-1 mRNA and protein expression in MM patients in a bid to evaluate possible benefits of their homology for immunotherapy. LAGE-1 (a and b isoforms) and NY-ESO-1 mRNA expression was studied in 18 normal tissues and 50 bone marrow MM samples by RT-PCR. LAGE-1 and NY-ESO-1 protein expression was analyzed by immunohistochemistry (IHC) in 27 MM specimens using mAbs 219-510-23 and E978. Spontaneous serological immune response against both antigens was analyzed by ELISA in sera from 33 MM patients. LAGE-1 (a and b isoforms) was positive in 42% and NY-ESO-1 in 26% of the MM samples analyzed by RT-PCR. Both genes were found to be expressed in 18% of the cases, while at least one of the genes was found to be expressed in 50% of the cases. In LAGE-1 positive samples, 81% were positive for LAGE-1a and 19% were positive for both LAGE-1a and -1b. LAGE-1 and NY-ESO-1 protein expression could only be detected in two cases by IHC and there was a clear strong spontaneous antibody response to LAGE-1 and NY-ESO-1 in only one MM patient. In conclusion, LAGE-1a and NY-ESO-1 homology cannot be easily exploited in an anti-NY-ESO-1 vaccine given the low frequency of protein expression detected by IHC or serum analysis.

  6. Integrated NY-ESO-1 antibody and CD8+ T-cell responses correlate with clinical benefit in advanced melanoma patients treated with ipilimumab.

    PubMed

    Yuan, Jianda; Adamow, Matthew; Ginsberg, Brian A; Rasalan, Teresa S; Ritter, Erika; Gallardo, Humilidad F; Xu, Yinyan; Pogoriler, Evelina; Terzulli, Stephanie L; Kuk, Deborah; Panageas, Katherine S; Ritter, Gerd; Sznol, Mario; Halaban, Ruth; Jungbluth, Achim A; Allison, James P; Old, Lloyd J; Wolchok, Jedd D; Gnjatic, Sacha

    2011-10-01

    Ipilimumab, a monoclonal antibody against cytotoxic T lymphocyte antigen 4 (CTLA-4), has been shown to improve survival in patients with advanced metastatic melanoma. It also enhances immunity to NY-ESO-1, a cancer/testis antigen expressed in a subset of patients with melanoma. To characterize the association between immune response and clinical outcome, we first analyzed NY-ESO-1 serum antibody by ELISA in 144 ipilimumab-treated patients with melanoma and found 22 of 140 (16%) seropositive at baseline and 31 of 144 (22%) seropositive following treatment. These NY-ESO-1-seropositive patients had a greater likelihood of experiencing clinical benefit 24 wk after ipilimumab treatment than NY-ESO-1-seronegative patients (P = 0.02, relative risk = 1.8, two-tailed Fisher test). To understand why some patients with NY-ESO-1 antibody failed to experience clinical benefit, we analyzed NY-ESO-1-specific CD4(+) and CD8(+) T-cell responses by intracellular multicytokine staining in 20 NY-ESO-1-seropositive patients and found a surprising dissociation between NY-ESO-1 antibody and CD8 responses in some patients. NY-ESO-1-seropositive patients with associated CD8(+) T cells experienced more frequent clinical benefit (10 of 13; 77%) than those with undetectable CD8(+) T-cell response (one of seven; 14%; P = 0.02; relative risk = 5.4, two-tailed Fisher test), as well as a significant survival advantage (P = 0.01; hazard ratio = 0.2, time-dependent Cox model). Together, our data suggest that integrated NY-ESO-1 immune responses may have predictive value for ipilimumab treatment and argue for prospective studies in patients with established NY-ESO-1 immunity. The current findings provide a strong rationale for the clinical use of modulators of immunosuppression with concurrent approaches to favor tumor antigen-specific immune responses, such as vaccines or adoptive transfer, in patients with cancer.

  7. Penta-BxNy sheet: a density functional theory study of two-dimensional material.

    PubMed

    Li, Jiao; Fan, Xinyu; Wei, Yanpei; Chen, Gang

    2016-01-01

    By using density functional theory with generalized gradient approximation, we have carried out detailed investigations of two-dimensional BxNy nanomaterials in the Cairo pentagonal tiling geometry fully composed of pentagons (penta-BxNy). Only penta-BN and BN2 planar structures are dynamically stable without imaginary modes in their phonon spectra. Their stabilities have been further evaluated by formation energy analysis, first-principles molecular dynamics simulation, and mechanical stability analysis. Penta-BN2 is superior to penta-BN in structural stability. Its stability analysis against oxidization and functional group adsorption as well as its synthesizing reaction path analysis show possibilities in fabricating penta-BN2 on experiment. Furthermore, the penta-BN2 could be transferred from metallic to semiconducting by ionizing or covalently binding an electron per dinitrogen. Also, it has been found to have superior mechanical properties, such as the negative Poisson's ratio and the comparable stiffness as that of hexagonal h-BN sheet. These studies on the stabilities, electronic properties, and mechanical properties suggest penta-BN2 as an attractive material to call for further studies on both theory and experiment. PMID:27539445

  8. Source assessment of atmospheric lead measured at Ny-Ålesund, Svalbard

    NASA Astrophysics Data System (ADS)

    Bazzano, Andrea; Ardini, Francisco; Becagli, Silvia; Traversi, Rita; Udisti, Roberto; Cappelletti, David; Grotti, Marco

    2015-07-01

    Size-segregated (PM10) aerosol samples were collected at Ny-Ålesund (Svalbard Islands, Norwegian Arctic) from April to September 2012 and analysed for lead content and isotopic composition (207Pb/206Pb and 208Pb/206Pb), along with other chemical tracers, such as aluminium (crustal marker) and non-sea-salt sulphates (anthropogenic and marine biogenic marker). It was found that most of lead reaching Ny-Ålesund is anthropogenic, with a marked seasonality of both the concentration and isotopic signature. Particularly, average lead concentration in summer was significantly lower than in spring (p = 0.003), whereas 208Pb/206Pb decreased from 2.107 ± 0.002 to 2.090 ± 0.005 (mean ± 95%-confidence level, p = 6.0 10-6). A comparison of the measured isotopic ratios to literature data suggests that the atmospheric lead reaching the Arctic during spring can be mainly related to inputs from eastern Eurasia, whereas North America appeared to be the major source during the summer. Experimental results and sampling strategy also indicate that local inputs of crustal and anthropogenic lead play a minor role. The source-receptor relationship was confirmed by a back-trajectory cluster analysis of air-masses reaching the sampling site.

  9. Lights out: Impact of the August 2003 power outage on mortality in New York, NY

    PubMed Central

    Anderson, G. Brooke; Bell, Michelle L.

    2012-01-01

    Background Little is known about how power outages affect health. We investigated mortality effects of the largest US blackout to date, August 14–15, 2003 in New York, NY. Methods We estimated mortality risk in New York, NY, using a generalized linear model with data from 1987–2005. We incorporated possible confounders, including weather and long-term and seasonal mortality trends. Results During the blackout, mortality increased for accidental deaths (122% [95% confidence interval = 28%–287%]) and non-accidental (i.e., disease-related) deaths (25% [12%–41%]), resulting in approximately 90 excess deaths. Increased mortality was not from deaths being advanced by a few days; rather, mortality risk remained slightly elevated through August 2003. Discussion To our knowledge, this is the first analysis of power outages and non-accidental mortality. Understanding the impact of power outages on human health is relevant, given that increased energy demand and climate change are likely to put added strain on power grids. PMID:22252408

  10. Structural, optical and electrical properties of WOxNy filmsdeposited by reactive dual magnetron sputtering

    SciTech Connect

    Mohamed, Sodky H.; Anders, Andre

    2006-06-05

    Thin films of tungsten oxynitride were prepared by dual magnetron sputtering of tungsten using argon/oxygen/nitrogen gas mixtures with various nitrogen/oxygen ratios. The presence of even small amounts of oxygen had a great effect not only on the composition but on the structure of WOxNy films, as shown by Rutherford backscattering and x-ray diffraction, respectively. Significant incorporation of nitrogen occurred only when the nitrogen partial pressure exceeded 89 percent of the total reactive gas pressure. Sharp changes in the stoichiometry, deposition rate, room temperature resistivity, electrical activation energy and optical band gap were observed when the nitrogen/oxygen ratio was high.The deposition rate increased from 0.31 to 0.89 nm/s, the room temperature resistivity decreased from 1.65 x 108 to 1.82 x 10-2 ?cm, the electrical activation energy decreased from 0.97 to 0.067 eV, and the optical band gap decreased from 3.19 to 2.94 eV upon nitrogen incorporation into the films. WOxNy films were highly transparent as long as the nitrogen incorporation was low, and were brownish (absorbing) and partially reflecting as nitrogen incorporation became significant.

  11. MAX-DOAS measurements of atmospheric trace gases in Ny-Ålesund

    NASA Astrophysics Data System (ADS)

    Wittrock, F.; Oetjen, H.; Richter, A.; Fietkau, S.; Medeke, T.; Rozanov, A.; Burrows, J. P.

    2003-12-01

    A new approach to derive tropospheric concentrations of some atmospheric trace gases from ground-based UV/vis measurements is described. The instrument, referred to as the MAX-DOAS, is based on the well-known UV/vis instruments, which use the sunlight scattered in the zenith sky as the light source and the method of Differential Optical Absorption Spectroscopy (DOAS) to derive column amounts of absorbers like ozone and nitrogen dioxide. Substantial enhancements have been applied to this standard setup to use different lines of sight near to the horizon as additional light sources (MAX - multi axis). Results from measurements at Ny-Ålesund (79° N, 12° E) are presented and interpreted with the full-spherical radiation transport model SCIATRAN. In particular, measurements of the oxygen dimer O4 which has a known column and vertical distribution in the atmosphere are used to evaluate the sensitivity of the retrieval to parameters such as multiple scattering, solar azimuth, surface albedo and refraction in the atmosphere and also to validate the radiative transport model. As a first application, measurements of NO2 emissions from a ship lying in Ny-Ålesund harbour are presented. The results of this study demonstrate the feasibility of long term UV/vis multi axis measurement that can be used to derive not only column amounts of different trace gases but also some information on the vertical location of these absorbers.

  12. Penta-BxNy sheet: a density functional theory study of two-dimensional material

    NASA Astrophysics Data System (ADS)

    Li, Jiao; Fan, Xinyu; Wei, Yanpei; Chen, Gang

    2016-08-01

    By using density functional theory with generalized gradient approximation, we have carried out detailed investigations of two-dimensional BxNy nanomaterials in the Cairo pentagonal tiling geometry fully composed of pentagons (penta-BxNy). Only penta-BN and BN2 planar structures are dynamically stable without imaginary modes in their phonon spectra. Their stabilities have been further evaluated by formation energy analysis, first-principles molecular dynamics simulation, and mechanical stability analysis. Penta-BN2 is superior to penta-BN in structural stability. Its stability analysis against oxidization and functional group adsorption as well as its synthesizing reaction path analysis show possibilities in fabricating penta-BN2 on experiment. Furthermore, the penta-BN2 could be transferred from metallic to semiconducting by ionizing or covalently binding an electron per dinitrogen. Also, it has been found to have superior mechanical properties, such as the negative Poisson’s ratio and the comparable stiffness as that of hexagonal h-BN sheet. These studies on the stabilities, electronic properties, and mechanical properties suggest penta-BN2 as an attractive material to call for further studies on both theory and experiment.

  13. Synthesis of Tin Nitride SnxNyNanowires by Chemical Vapour Deposition

    PubMed Central

    2009-01-01

    Tin nitride (SnxNy) nanowires have been grown for the first time by chemical vapour deposition on n-type Si(111) and in particular by nitridation of Sn containing NH4Cl at 450 °C under a steady flow of NH3. The SnxNynanowires have an average diameter of 200 nm and lengths ≥5 μm and were grown on Si(111) coated with a few nm’s of Au. Nitridation of Sn alone, under a flow of NH3is not effective and leads to the deposition of Sn droplets on the Au/Si(111) surface which impedes one-dimensional growth over a wide temperature range i.e. 300–800 °C. This was overcome by the addition of ammonium chloride (NH4Cl) which undergoes sublimation at 338 °C thereby releasing NH3and HCl which act as dispersants thereby enhancing the vapour pressure of Sn and the one-dimensional growth of SnxNynanowires. In addition to the action of dispersion, Sn reacts with HCl giving SnCl2which in turn reacts with NH3leading to the formation of SnxNyNWs. A first estimate of the band-gap of the SnxNynanowires grown on Si(111) was obtained from optical reflection measurements and found to be ≈2.6 eV. Finally, intricate assemblies of nanowires were also obtained at lower growth temperatures. PMID:20596341

  14. Penta-BxNy sheet: a density functional theory study of two-dimensional material

    PubMed Central

    Li, Jiao; Fan, Xinyu; Wei, Yanpei; Chen, Gang

    2016-01-01

    By using density functional theory with generalized gradient approximation, we have carried out detailed investigations of two-dimensional BxNy nanomaterials in the Cairo pentagonal tiling geometry fully composed of pentagons (penta-BxNy). Only penta-BN and BN2 planar structures are dynamically stable without imaginary modes in their phonon spectra. Their stabilities have been further evaluated by formation energy analysis, first-principles molecular dynamics simulation, and mechanical stability analysis. Penta-BN2 is superior to penta-BN in structural stability. Its stability analysis against oxidization and functional group adsorption as well as its synthesizing reaction path analysis show possibilities in fabricating penta-BN2 on experiment. Furthermore, the penta-BN2 could be transferred from metallic to semiconducting by ionizing or covalently binding an electron per dinitrogen. Also, it has been found to have superior mechanical properties, such as the negative Poisson’s ratio and the comparable stiffness as that of hexagonal h-BN sheet. These studies on the stabilities, electronic properties, and mechanical properties suggest penta-BN2 as an attractive material to call for further studies on both theory and experiment. PMID:27539445

  15. Gulf/RTR oil sands extraction process. [Gulf/Rio Tinto TIL Holding S. A

    SciTech Connect

    Logan, A.; Devenny, D.; Porcari, G.; Corti, A.

    1984-06-01

    The activities carried out and the results obtained from a 15 tons/hour oil sands extraction pilot plant operated in Fort McMurray in Northern Alberta are described. The process is the Rio Tinto TIL Holding S.A. (RTR)/Gulf Canada Lt. Oil Sands Extraction Process. It is a modified hot water extraction process. It is used to extract bitumen from Athabasca oil sands. The test ran from July to December 1981 through ambient conditions ranging from plus 38/sup 0/C to minus 30/sup 0/C (100/sup 0/F to -22/sup 0/F). The process, the on-site facilities, the test program, an analysis of plant performance, an appraisal of the process economics, and an evaluation of its potential application are described.

  16. Resiquimod as an immunologic adjuvant for NY-ESO-1 protein vaccination in patients with high-risk melanoma.

    PubMed

    Sabado, Rachel Lubong; Pavlick, Anna; Gnjatic, Sacha; Cruz, Crystal M; Vengco, Isabelita; Hasan, Farah; Spadaccia, Meredith; Darvishian, Farbod; Chiriboga, Luis; Holman, Rose Marie; Escalon, Juliet; Muren, Caroline; Escano, Crystal; Yepes, Ethel; Sharpe, Dunbar; Vasilakos, John P; Rolnitzsky, Linda; Goldberg, Judith D; Mandeli, John; Adams, Sylvia; Jungbluth, Achim; Pan, Linda; Venhaus, Ralph; Ott, Patrick A; Bhardwaj, Nina

    2015-03-01

    The Toll-like receptor (TLR) 7/8 agonist resiquimod has been used as an immune adjuvant in cancer vaccines. We evaluated the safety and immunogenicity of the cancer testis antigen NY-ESO-1 given in combination with Montanide (Seppic) with or without resiquimod in patients with high-risk melanoma. In part I of the study, patients received 100 μg of full-length NY-ESO-1 protein emulsified in 1.25 mL of Montanide (day 1) followed by topical application of 1,000 mg of 0.2% resiquimod gel on days 1 and 3 (cohort 1) versus days 1, 3, and 5 (cohort 2) of a 21-day cycle. In part II, patients were randomized to receive 100-μg NY-ESO-1 protein plus Montanide (day 1) followed by topical application of placebo gel [(arm A; n = 8) or 1,000 mg of 0.2% resiquimod gel (arm B; n = 12)] using the dosing regimen established in part I. The vaccine regimens were generally well tolerated. NY-ESO-1-specific humoral responses were induced or boosted in all patients, many of whom had high titer antibodies. In part II, 16 of 20 patients in both arms had NY-ESO-1-specific CD4⁺ T-cell responses. CD8⁺ T-cell responses were only seen in 3 of 12 patients in arm B. Patients with TLR7 SNP rs179008 had a greater likelihood of developing NY-ESO-1-specific CD8⁺ responses. In conclusion, NY-ESO-1 protein in combination with Montanide with or without topical resiquimod is safe and induces both antibody and CD4⁺ T-cell responses in the majority of patients; the small proportion of CD8⁺ T-cell responses suggests that the addition of topical resiquimod to Montanide is not sufficient to induce consistent NY-ESO-1-specific CD8⁺ T-cell responses.

  17. 33 CFR 207.610 - St. Lawrence River, Cape Vincent Harbor, N.Y.; use, administration, and navigation of the harbor...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false St. Lawrence River, Cape Vincent Harbor, N.Y.; use, administration, and navigation of the harbor and U.S. breakwater. 207.610 Section 207... NAVIGATION REGULATIONS § 207.610 St. Lawrence River, Cape Vincent Harbor, N.Y.; use, administration,...

  18. Allorestricted T lymphocytes with a high avidity T-cell receptor towards NY-ESO-1 have potent anti-tumor activity.

    PubMed

    Krönig, Holger; Hofer, Kathrin; Conrad, Heinke; Guilaume, Philippe; Müller, Julia; Schiemann, Matthias; Lennerz, Volker; Cosma, Antonio; Peschel, Christian; Busch, Dirk H; Romero, Pedro; Bernhard, Helga

    2009-08-01

    The cancer-testis antigen NY-ESO-1 has been targeted as a tumor-associated antigen by immunotherapeutical strategies, such as cancer vaccines. The prerequisite for a T-cell-based therapy is the induction of T cells capable of recognizing the NY-ESO-1-expressing tumor cells. In this study, we generated human T lymphocytes directed against the immunodominant NY-ESO-1(157-165) epitope known to be naturally presented with HLA-A*0201. We succeeded to isolate autorestricted and allorestricted T lymphocytes with low, intermediate or high avidity TCRs against the NY-ESO-1 peptide. The avidity of the established CTL populations correlated with their capacity of lysing HLA-A2-positive, NY-ESO-1-expressing tumor cell lines derived from different origins, e.g. melanoma and myeloma. The allorestricted NY-ESO-1-specific T lymphocytes displayed TCRs with the highest avidity and best anti-tumor recognition activity. TCRs derived from allorestricted, NY-ESO-1-specific T cells may be useful reagents for redirecting primary T cells by TCR gene transfer and, therefore, may facilitate the development of adoptive transfer regimens based on TCR-transduced T cells for the treatment of NY-ESO-1-expressing hematological malignancies and solid tumors.

  19. 77 FR 13263 - Foreign-Trade Zone 109 - Watertown, NY; Amendment to Application; North American Tapes, LLC...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... number 48-2011 on July 15, 2011 (76 FR 43259, 7- 20-2011). The NAT facility (25 employees) is located... American Tapes, LLC (Textile Athletic Tape), Watertown, NY A request has been submitted to the Foreign... used to produce pressure-sensitive adhesive athletic tape with textile fabric backing material for...

  20. 77 FR 65015 - Notice of Intent To Repatriate Cultural Items: American Museum of Natural History, New York, NY...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-24

    ... National Park Service Notice of Intent To Repatriate Cultural Items: American Museum of Natural History... Resources, American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024... after that date if no additional claimants come forward. The American Museum of......

  1. 33 CFR 165.164 - Security Zones: Dignitary Arrival/Departure and United Nations Meetings, New York, NY.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .../Departure and United Nations Meetings, New York, NY. 165.164 Section 165.164 Navigation and Navigable Waters... Guard District § 165.164 Security Zones: Dignitary Arrival/Departure and United Nations Meetings, New... information broadcasts. (b) Regulations. (1) The general regulations contained in 33 CFR 165.33 apply. (2)...

  2. 76 FR 61747 - CompONE Services, LTD, Ithaca, NY; Notice of Negative Determination Regarding Application for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ... Employment and Training Administration CompONE Services, LTD, Ithaca, NY; Notice of Negative Determination... administrative reconsideration of the negative ] determination regarding workers' eligibility to apply for Trade... York (CompONE Services). The negative determination was issued on August 3, 2011. The...

  3. 77 FR 44683 - Phillips-Van Heusen Corporation, IZOD Dress Furnishings Division, New York, NY; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-30

    ... York, NY; Notice of Negative Determination on Reconsideration On May 21, 2012, the Department of Labor... Department's Notice of determination was published in the Federal Register on April 19, 2012 (77 FR 23511... Assistance (TAA) investigation resulted in a negative determination based on the findings that with...

  4. FIELD PERFORMANCE OF ADVANCED TECHNOLOGY WOODSTOVES IN GLEN FALLS, NY, 1988-89 - VOLUME II - TECHNICAL APPENDICES

    EPA Science Inventory

    The report gives results of an evaluation of particulate emission trends for three models of catalytic and two models of non- catalytic woodstoves under "in-home" burning conditions during the 1988-89 heating season in Glens Falls, NY. The results (averaging 9.4 g/h and 9.4 g/kg...

  5. 78 FR 62293 - Safety Zone, Oyster Festival 30th Anniversary Fireworks Display, Oyster Bay; Oyster Bay, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-10-15

    ... Captain of the Port DHS Department of Homeland Security FR Federal Register NPRM Notice of Proposed... CFR Part 165 RIN 1625-AA00 Safety Zone, Oyster Festival 30th Anniversary Fireworks Display, Oyster Bay; Oyster Bay, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard...

  6. 77 FR 43158 - Special Local Regulation; Battle on the Bay Powerboat Race Atlantic Ocean, Fire Island, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-24

    .... SUPPLEMENTARY INFORMATION: Table of Acronyms COTP Captain of the Port DHS Department of Homeland Security FR...; Patchogue Bay, Patchogue, NY, in the Federal Register (73 FR 51367) establishing a safety zone on Patchogue...; Marine Events in Captain of the Port Long Island Sound Zone in the Federal Register (76 FR...

  7. 76 FR 66685 - Foreign-Trade Zone 37-Orange County, NY, Application for Subzone, ITT Water Technology, Inc...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-27

    ... Technology, Inc. (Centrifugal and Submersible Pumps), Auburn, NY An application has been submitted to the... special purpose subzone status for the centrifugal and submersible pump manufacturing and warehousing... facilities are used to manufacture and distribute centrifugal and submersible pumps and related...

  8. Development and Initial Validation of the NyTid Test: A Movement Assessment Tool for Compulsory School Pupils

    ERIC Educational Resources Information Center

    Tidén, Anna; Lundqvist, Carolina; Nyberg, Marie

    2015-01-01

    This study presents the development process and initial validation of the NyTid test, a process-oriented movement assessment tool for compulsory school pupils. A sample of 1,260 (627 girls and 633 boys; mean age of 14.39) Swedish school children participated in the study. In the first step, exploratory factor analyses (EFAs) were performed in…

  9. 33 CFR 165.159 - Safety Zone: New York Air Show at Jones Beach State Park, Wantagh, NY.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... following waters of the Atlantic Ocean off of Jones Beach State Park, Wantagh, NY are designated a safety... position 40°35′49″ N, 073°28′47″ W; then running south to a position in the Atlantic Ocean off of...

  10. 33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Black Rock Channel opposite foot... Channel opposite foot of Porter Avenue, Buffalo, N.Y. An area extending northwesterly between Black Rock Channel and Bird Island Pier opposite the foot of Porter Avenue, bounded as follows: Beginning...

  11. 33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Black Rock Channel opposite foot... Channel opposite foot of Porter Avenue, Buffalo, N.Y. An area extending northwesterly between Black Rock Channel and Bird Island Pier opposite the foot of Porter Avenue, bounded as follows: Beginning...

  12. 33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Black Rock Channel opposite foot... Channel opposite foot of Porter Avenue, Buffalo, N.Y. An area extending northwesterly between Black Rock Channel and Bird Island Pier opposite the foot of Porter Avenue, bounded as follows: Beginning...

  13. 33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Black Rock Channel opposite foot... Channel opposite foot of Porter Avenue, Buffalo, N.Y. An area extending northwesterly between Black Rock Channel and Bird Island Pier opposite the foot of Porter Avenue, bounded as follows: Beginning...

  14. Examination of Below-Ground Structure and Soil Respiration Rates of Stable and Deteriorating Salt Marshes in Jamaica Bay (NY)

    EPA Science Inventory

    CAT scan imaging is currently being used to examine below-ground peat and root structure in cores collected from salt marshes of Jamaica Bay, part of the Gateway National Recreation Area (NY). CAT scans or Computer-Aided Tomography scans use X-ray equipment to produce multiple i...

  15. 77 FR 37569 - Establishment of Class D Airspace and Amendment of Class E Airspace; East Hampton, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-22

    ... Hampton, NY, to accommodate a new air traffic control tower at East Hampton Airport (77 FR 15297... establishes higher weather minima for VFR flights, thus restricting access of VFR flights to the airspace... Class D airspace during adverse weather conditions. While the FAA agrees that one-at-a-time Special...

  16. 76 FR 38383 - Albany Engineering Corporation, Town of Stuyvesant, NY; Notice of Site Visit and Technical Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-30

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Albany Engineering Corporation, Town of Stuyvesant, NY; Notice of Site Visit and Technical Meeting On July 12, 2011, Office of Energy Projects staff will participate in a...

  17. 77 FR 63290 - Foreign-Trade Zone 121-Albany, NY; Notification of Proposed Production Activity; Albany Molecular...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-16

    ... Rensselaer, New York, (Board Order 698, 7/20/1994, 59 FR 18318, 7/28/1994). AMRI is now requesting to produce...; Albany Molecular Research, Inc., Subzone 121A, (Pharmaceutical Chemicals Production), Rensselaer, NY... originally approved by the Board in 1994 for the production of bulk pharmaceutical chemicals...

  18. Exploring the feasibility of Sclerotium rolfsii VrNY as a potential bioherbicide for control of Swallowworts (Vincetoxicum spp.)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pale swallowwort (PSW) and black swallowwort (BSW) are two viney milkweeds native to Europe that have increasingly become problematic and noxious weeds in eastern North America. An indigenous fungal isolate, Sclerotium rolfsii VrNY, was discovered causing significant mortality in a dense stand of PS...

  19. 33 CFR 100.121 - Swim Across the Sound, Long Island Sound, Port Jefferson, NY to Captain's Cove Seaport...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Swim Across the Sound, Long... SAFETY OF LIFE ON NAVIGABLE WATERS § 100.121 Swim Across the Sound, Long Island Sound, Port Jefferson, NY... they are officially participating in the Swim Across the Sound event or are otherwise authorized by...

  20. 33 CFR 100.121 - Swim Across the Sound, Long Island Sound, Port Jefferson, NY to Captain's Cove Seaport...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Swim Across the Sound, Long... SAFETY OF LIFE ON NAVIGABLE WATERS § 100.121 Swim Across the Sound, Long Island Sound, Port Jefferson, NY... they are officially participating in the Swim Across the Sound event or are otherwise authorized by...

  1. Cancer-testis antigens PRAME and NY-ESO-1 correlate with tumour grade and poor prognosis in myxoid liposarcoma.

    PubMed

    Iura, Kunio; Kohashi, Kenichi; Hotokebuchi, Yuka; Ishii, Takeaki; Maekawa, Akira; Yamada, Yuichi; Yamamoto, Hidetaka; Iwamoto, Yukihide; Oda, Yoshinao

    2015-07-01

    Myxoid liposarcoma is the second most common liposarcoma. Although myxoid liposarcoma is relatively chemosensitive and thus a good candidate for chemotherapy, cases with relapsed or metastatic disease still have poor outcome. Here, we performed a gene microarray analysis to compare the gene expression profiles in six clinical myxoid liposarcoma samples and three normal adipose tissue samples, and to identify molecular biomarkers that would be useful as diagnostic markers or treatment targets in myxoid liposarcoma. This showed that the cancer-testis antigen PRAME was up-regulated in myxoid liposarcoma. We then performed immunohistochemical, western blotting and real-time polymerase chain reaction analyses to quantify the expression of PRAME and another cancer-testis antigen, NY-ESO-1, in clinical samples of myxoid liposarcoma (n = 93), dedifferentiated (n = 46), well-differentiated (n = 32) and pleomorphic liposarcomas (n = 14). Immunohistochemically, positivity for PRAME and NY-ESO-1 was observed in 84/93 (90%) and 83/93 (89%) of the myxoid liposarcomas, and in 20/46 (43%) and 3/46 (7%) of the dedifferentiated, 3/32 (9%) and 1/32 (3%) of the well-differentiated and 7/14 (50%) and 3/21 (21%) of the pleomorphic liposarcomas, respectively. High immunohistochemical expression of PRAME and/or NY-ESO-1 was significantly correlated with tumour diameter, the existence of tumour necrosis, a round-cell component of >5%, higher histological grade and advanced clinical stage. High PRAME and NY-ESO-1 expression correlated significantly with poor prognosis in a univariate analysis. The myxoid liposarcomas showed significantly higher protein and mRNA expression levels of PRAME and NY-ESO-1 (CTAG1B) than the other liposarcomas. In conclusion, PRAME and NY-ESO-1 (CTAG1B) were expressed in the vast majority of myxoid liposarcomas, and their high-level expression correlated with tumour grade and poor prognosis. Our results support the potential use of PRAME and NY

  2. Ab initio study of small Au nY 2 ( n=1-4) clusters

    NASA Astrophysics Data System (ADS)

    Jian-Jun, Guo; Ji-Xian, Yang; Dong, Die

    2008-11-01

    The geometries of the lowest-lying isomers of Au nY 2 ( n=1-4) clusters are determined systematically via the density functional method B3LYP with relativistic effective core potentials (RECP) and LANL2DZ basis set. Several low-lying isomers were determined, and many of them in electronic configurations with a high-spin multiplicity. The gold-yttrium interaction is strong enough to modify the known growth pattern of bare gold clusters. The stability trend of Y-doped Au n clusters is compared to that of pure Au n clusters. The results show that the inclusion of two Y atoms in the clusters improves the cluster stability, and indicate higher stability as the structures grow in size. The present calculations are useful to understand the enhanced catalytic activity and selectivity gained by using yttrium-doped gold catalyst.

  3. Thermal conductivity reduction by interface roughness in AlNx-GaNy superlattices.

    NASA Astrophysics Data System (ADS)

    Kan Koh, Yee; Cao, Yu; Cahill, David; Jena, Debdeep

    2008-03-01

    A reduction of cross-plane thermal conductivity λ by a factor of two is achieved in AlN4 nm-GaN52 nm superlattices by varying the plasma power during growth. This reduction is attributed to interface roughness, introducing a new parameter to be considered in the design and fabrication of superlattices for thermoelectric applications. Thermal conductivity of AlNx-GaNy superlattices, x ˜ 4 nm and 2 < y < 1000 nm, with rough interfaces is then measured by time-domain thermoreflectance. λ decreases monotonically as the GaN thickness y decreases, λ = 6.35 W m-1 K-1 at y = 2.2 nm. We observe no minimum thermal conductivity as a function of period for these rough superlattices. A continuum model incorporating the effects of interface roughness indicates that diffuse scattering is dominant when y > 20 nm, and significant coherent transmission occurs when y < 20 nm.

  4. Distributions and impact factors of antimony in topsoils and moss in Ny-Ålesund, Arctic.

    PubMed

    Jia, Nan; Sun, Liguang; He, Xin; You, Kehua; Zhou, Xin; Long, Nanye

    2012-12-01

    The distribution of antimony (Sb) in topsoil and moss (Dicranum angustum) in disturbed and undisturbed areas, as well as coal and gangue, in Ny-Ålesund, Arctic was examined. Results show that the weathering of coal bed could not contribute to the increase of Sb concentrations in topsoil and moss in the study area. The distribution of Sb is partially associated with traffic and historical mining activities. The occurrence of the maximum Sb concentration is due to the contribution of human activities. In addition, the decrease of Sb content in topsoil near the coastline may be caused by the washing of seawater. Compared with topsoils, moss could be a useful tool for monitoring Sb in both highly and lightly polluted areas.

  5. Antibiotic usage in 2013 on a dairy CAFO in NY State, USA.

    PubMed

    Doane, Marie; Sarenbo, Sirkku

    2014-01-01

    Antimicrobial resistance is threatening humans and animals worldwide. Biosecurity and 1-year usage of antibiotics on a dairy concentrated animal feeding operation (CAFO) in NY State, USA, were mapped: how much antibiotics were used, for what purpose, and whether any decrease could be warranted. Approximately 493 kg antibiotics was used, of which 376 kg was ionophores (monensin and lasalocides), 79 kg penicillin, 16.5 kg lincosamides, 8.0 kg aminoglycosides, 7.7 kg sulfamides, 3.4 kg cephalosporin, 2 kg macrolides, 0.7 kg amphenicols, and 0.1 kg fluoroquinolones. Usage reduction by 84% was realistic without compromising the animal welfare. Further reduction could be possible by improving the biosecurity and by utilizing antibiotic sensitivity testing. PMID:24891936

  6. Antibiotic usage in 2013 on a dairy CAFO in NY State, USA

    PubMed Central

    Doane, Marie; Sarenbo, Sirkku

    2014-01-01

    Antimicrobial resistance is threatening humans and animals worldwide. Biosecurity and 1-year usage of antibiotics on a dairy concentrated animal feeding operation (CAFO) in NY State, USA, were mapped: how much antibiotics were used, for what purpose, and whether any decrease could be warranted. Approximately 493 kg antibiotics was used, of which 376 kg was ionophores (monensin and lasalocides), 79 kg penicillin, 16.5 kg lincosamides, 8.0 kg aminoglycosides, 7.7 kg sulfamides, 3.4 kg cephalosporin, 2 kg macrolides, 0.7 kg amphenicols, and 0.1 kg fluoroquinolones. Usage reduction by 84% was realistic without compromising the animal welfare. Further reduction could be possible by improving the biosecurity and by utilizing antibiotic sensitivity testing. PMID:24891936

  7. Polar cloud observatory at Ny-Ålesund in GRENE Arctic Climate Change Research Project

    NASA Astrophysics Data System (ADS)

    Yamanouchi, Takashi; Takano, Toshiaki; Shiobara, Masataka; Okamoto, Hajime; Koike, Makoto; Ukita, Jinro

    2016-04-01

    Cloud is one of the main processes in the climate system and especially a large feed back agent for Arctic warming amplification (Yoshimori et al., 2014). From this reason, observation of polar cloud has been emphasized and 95 GHz cloud profiling radar in high precision was established at Ny-Ålesund, Svalbard in 2013 as one of the basic infrastructure in the GRENE (Green Network of Excellence Program) Arctic Climate Change Research Project. The radar, "FALCON-A", is a FM-CW (frequency modulated continuous wave) Doppler radar, developed for Arctic use by Chiba University (PI: T. Takano) in 2012, following its prototype, "FALCON-1" which was developed in 2006 (Takano et al., 2010). The specifications of the radar are, central frequency: 94.84 GHz; antenna power: 1 W; observation height: up to 15 km; range resolution: 48 m; beam width: 0.2 degree (15 m at 5 km); Doppler width: 3.2 m/s; time interval: 10 sec, and capable of archiving high sensitivity and high spatial and time resolution. An FM-CW type radar realizes similar sensitivity with much smaller parabolic antennas separated 1.4 m from each other used for transmitting and receiving the wave. Polarized Micro-Pulse Lidar (PMPL, Sigma Space MPL-4B-IDS), which is capable to measure the backscatter and depolarization ratio, has also been deployed to Ny-Ålesund in March 2012, and now operated to perform collocated measurements with FALCON-A. Simultaneous measurement data from collocated PMPL and FALCON-A are available for synergetic analyses of cloud microphysics. Cloud mycrophysics, such as effective radius of ice particles and ice water content, are obtained from the analysis based on algorithm, which is modified for ground-based measurements from Okamoto's retrieval algorithm for satellite based cloud profiling radar and lidar (CloudSat and CALIPSO; Okamoto et al., 2010). Results of two years will be shown in the presentation. Calibration is a point to derive radar reflectivity (dBZ) from original intensity data

  8. Bárány's theory is right, but incomplete. An experimental study in pigeons.

    PubMed

    Wit, H P; Spoelstra, H A; Segenhout, J M

    1990-01-01

    The horizontal semicircular canal in pigeons was thermally stimulated with a miniature Peltier-element device. Sound evoked electric responses from the vestibular organ were modulated by the caloric stimulus. Experiments were performed under minimal influence of gravity, (horizontal canals in horizontal plane), and with the pigeons head tilted forwards or backwards. On the basis of the results it was concluded that the influence of gravity can be described by Bárány's convection theory. Extra effects are, however, present and they are most probably partly of mechanical origin (fluid expansion) and partly due to a direct influence of temperature on the vestibular receptors. Further experiments are needed to investigate the origin of these extra effects in more detail. PMID:2386023

  9. Chromosomal localization of TIL, a gene encoding a protein related to the Drosophila transmembrane receptor Toll, to human chromosome 4p14

    SciTech Connect

    Taguchi, Takahiro; Testa, J.R.; Mitcham, J.L.; Dower, S.K.; Sims, J.E.

    1996-03-05

    This report describes the localization of the the TIL gene to human chromosome 4p14 using fluorescence in situ hybridization. This gene encodes a protein which is related to the Drosophila transmembrane receptor Toll and the mammalian interleukin-1 receptor, which share similarities in structure and function. The Drosophila gene is also important during embryonic development, which makes TIL a candidate locus for human congenital malformations that are genetically linked to human chromosome 4. 17 refs., 1 fig.

  10. [Records and sources of mercury pollution over the past 200 years in Ny-Alesund, Arctic].

    PubMed

    Jiang, Shan; Liu, Xiao-Dong; Liu, Nan; Sun, Li-Guang

    2010-09-01

    Hg pollution has become a global problem since the industrial revolution. In this study,we collected a pond sediment core rich in moss residues from the Ny-Alesund of Svalbard, and determined the content and flux of Hg in the sediments. Combined with 210Pb and 137Cs dating,we reconstructed the changes of Hg concentration and depositional flux in the recent 200 years,and focused on the main controlling factors on the elevated inputs of Hg since the 1850s. The results show that the Hg contents increase significantly since the World Industrial Revolution. The highest value of Hg is up to 180 ng x g(-1) in the surface sediments, and the ratios of anthropogenic Hg increase from 30% to 90%, thus the input of anthropogenic Hg has become a main source of total mercury in the study area. The range of Hg deposition flux is between 8-20 microg x (m(2) x a)(-1) in past 200 years, and show three distinct peaks,corresponding to 1800 s, 1880 s and 1970 s. Compared the historical Hg deposition flux with the records of local coal combustion and globe Hg production, we suggest that the anthropogenic Hg is mainly derived from the long-range atmosphere Hg transport, whereas the influence of local coal mining is likely minor. The Ny-Alesund has similar Hg pollution degree with Sweden, Canada and other circum-arctic areas, but obviously higher than Greenland and significantly lower than America and Russia,as well as other industrialized countries.

  11. Arctic summer aerosols: a case study of Ny-Ålesund

    NASA Astrophysics Data System (ADS)

    Zhan, J.; Gao, Y.; Li, W.; Chen, L.

    2013-12-01

    Concentration of trace elements, ionic species and carbonaceous aerosol were investigated at Ny-Ålesund to assess the aerosols' origins and their impact on the chemical composition in the summer Arctic atmosphere. Air mass influenced by ship emissions was identified by elevated concentrations of non-crustal (nc) Vanadium (V), nc nickel (Ni), non-sea salt (nss) sulfate (SO42-) and by the ratios of nc-Ni/nc-V (1.7) and nss-SO42-/nc-V (200). The elevated Total Suspended Particulate (TSP) was observed when large cruise ships visited Ny-Ålesund during the observation period. The concentration was 2291 ng m-3, almost triple of the mean concentration (898.0 ng m-3). Elevated trace elements, as well as sulfate and nitrate, were observed in the same air mass. This suggests that ship emissions contributed significantly to Arctic atmospheric particulate matter. Additionally, backward trajectories and the ratio of nc manganese (Mn)/nc-V were used to identify the air mass'origins, which were Central Russia and the North Atlantic Ocean. However, the chemical compositions in these air masses were not significantly different from the composition of masses that originated in the Arctic Ocean. All the samples were enriched with Ni, copper (Cu), zinc(Zn), selenium (Se), silver(Ag), cadmium(Cd), antimony(Sb), thallium(Tl), even the sample collected from the Arctic Ocean air mass. Our limited data indicated that long-range transport didn't significantly contribute to the area' s atmospheric pollution, but local sources, such as transportation, coal mining, coal burning and energy production, were important contributors of heavy metals to the atmosphere, epically ship emissions.

  12. Fine-mapping naturally occurring NY-ESO-1 antibody epitopes in melanoma patients' sera using short overlapping peptides and full-length recombinant protein.

    PubMed

    Komatsu, Nobukazu; Jackson, Heather M; Chan, Kok-fei; Oveissi, Sara; Cebon, Jonathan; Itoh, Kyogo; Chen, Weisan

    2013-07-01

    The tumor antigen NY-ESO-1 is one of the most antigenic cancer-testis antigens, first identified by serologic analysis of a recombinant cDNA expression library (SEREX). NY-ESO-1 is expressed in different types of cancers including melanoma. NY-ESO-1-specific spontaneous humoral and cellular immune responses are detected in a large proportion of patients with advanced NY-ESO-1-expressing cancers. Therefore NY-ESO-1 is a good candidate antigen for immunotherapy. Although cellular immune responses to NY-ESO-1 are well characterized, much less is known about the humoral immune responses. In this study, we finely mapped linear antibody epitopes using sera from melanoma patients and shorter overlapping peptide sets. We have shown that melanoma patients' humoral immune systems responded to NY-ESO-1 differently in each individual with widely differing antibody specificity, intensity and antibody subtypes. This knowledge will help us further understand anti-tumor immunity and may also help us to monitor cancer progress and cancer vaccine efficacy in the future.

  13. til-1: a novel proviral insertion locus for Moloney murine leukaemia virus in lymphomas of CD2-myc transgenic mice.

    PubMed

    Stewart, M; Terry, A; O'Hara, M; Cameron, E; Onions, D; Neil, J C

    1996-03-01

    Moloney murine leukaemia virus (MoMLV) markedly accelerates thymic lymphoma development in mice carrying a transgene in which the human c-myc gene is linked to the CD2 locus control region. To investigate the mechanism of synergy and identify the genes which collaborate with myc in these clonal tumours, we analysed the sites of MoMLV insertion. Analysis of known viral integration loci revealed only a small number of insertions at bmi-1, pim-1 and ahi-1. Further cloning and hybridization analysis revealed a new common integration locus, designated til-1, which was occupied in 25 out of 77 lymphomas examined, with evidence of multiple clonal insertions in some cases. Mapping relative to established chromosomal markers in interspecific backcross mice located til-1 to mouse chromosome 17, distal to pim-1 and tic-1. These results suggest that the til-1 locus may harbour a novel myc-collaborating gene which acts as a target for activation in T cell lymphomas. PMID:8601779

  14. The cancer-testis antigen NY-ESO-1 is highly expressed in myxoid and round cell subset of liposarcomas.

    PubMed

    Hemminger, Jessica A; Ewart Toland, Amanda; Scharschmidt, Thomas J; Mayerson, Joel L; Kraybill, William G; Guttridge, Denis C; Iwenofu, O Hans

    2013-02-01

    Liposarcomas are a heterogenous group of fat-derived sarcomas, and surgery with or without chemoradiation therapy remains the main stay of treatment. NY-ESO-1 is a cancer-testis antigen expressed in various cancers where it can induce both cellular and humoral immunity. Immunotherapy has shown promise in clinical trials involving NY-ESO-1-expressing tumors. Gene expression studies have shown upregulation of the gene for NY-ESO-1, CTAG1B, in myxoid and round cell liposarcomas. Herein, we evaluated the expression of NY-ESO-1 among liposarcoma subtypes by quantitative real-time PCR, western blot analysis, and immunohistochemistry. Frozen tissue for quantitative real-time PCR and western blot analysis was obtained for the following liposarcoma subtypes (n=15): myxoid and round cell (n=8); well-differentiated (n=4), and dedifferentiated (n=3). Formalin-fixed paraffin-embedded blocks were obtained for the following liposarcoma subtypes (n=44): myxoid and round cell (n=18); well-differentiated (n=10); dedifferentiated (n=10); and pleomorphic (n=6). Full sections were stained with monoclonal antibody NY-ESO-1, and staining was assessed for intensity (1-3+), percentage of tumor positivity, and location. In all, 7/8 (88%) and 16/18 (89%) myxoid and round cell expressed CTAG1B and NY-ESO-1 by quantitative real-time PCR and immunohistochemistry, respectively. Western blot correlated with mRNA expression levels. By immunohistochemistry, 94% (15/16) of positive cases stained homogenously with 2-3+ intensity. Also, 3/6 (50%) pleomorphic liposarcomas demonstrated a range of staining: 1+ intensity in 50% of cells; 2+ intensity in 5% of cells; and 3+ intensity in 90% of cells. One case of dedifferentiated liposarcoma showed strong, diffuse staining (3+ intensity in 75% of cells). Our study shows that both CTAG1B mRNA and protein are overexpressed with high frequency in myxoid and round cell liposarcoma, enabling the potential use of targeted immunotherapy in the treatment of this

  15. Uplift history of the Villány Hills (SW Hungary) based on paleontological data

    NASA Astrophysics Data System (ADS)

    Sebe, Krisztina; Pazonyi, Piroska; Gasparik, Mihály; Lajos Szujó, Gábor

    2015-04-01

    Villány Hills are the southernmost basement outcrop in Hungary, along the northern margin of the Drava Basin. They comprise part of a Cretaceous nappe in the Tisza structural unit. Data on their young tectonic evolution are scarce, mostly due to the almost complete lack of Cenozoic sediments. Though somewhat debated, indirect data suggest that most of the area was covered by Upper Miocene (Lake Pannon) clastic sediments. Uplift and exhumation of the basement was driven by Late Miocene to post-Miocene basin inversion. A unique opportunity to study the uplift history is offered by paleontological data, by small but numerous terrestrial vertebrate sites, which cover a long time span in the Pliocene and Pleistocene. Faunas accumulated in fissure fillings of karstified limestone and thus provide a minimum age for the subaerial exposure (exhumation) of the bedrock at the given site. For some sites it has been proposed that water-linked species in the fauna refer to the existence of an alluvial plain on the clastic cover, in immediate proximity to the bedrock. If true, this would give an exact elevation of the surrounding terrain at the time indicated by the fauna. If exhumation of the basement happened during the time interval covered by the vertebrate faunas, then a relationship is expected to exist between the age and elevation of the the fossil sites, with the youngest faunas lying at lowest elevation. This relationship is expected to be strictly valid primarily in the case of water-linket faunas, but as a trend also for all fossil sites. We collected the age data and ecological requirements of the reliably datable vertebrate faunas and their locations with the possible precision. Then the relationship between the elevation and age was studied and compared to the available geomorphological, sedimentological and tectonic data. The age-elevation distribution of vertebrate faunas did not show the expected relationship. Based on the results, exhumation of the Villány

  16. PIXE Analysis of Atmospheric Aerosol Samples in an Urban Area in Upstate NY

    NASA Astrophysics Data System (ADS)

    Nadareski, Benjamin; Ali, Salina; Yoskowitz, Josh; Vineyard, Michael; Labrake, Scott

    2014-09-01

    Extremely fine particles (PM2.5) are found to penetrate deep into the lungs and hence, are found to have harmful health effects on humans. Atmospheric aerosol samples collected in Schenectady, NY were analyzed for evidence for air pollution; specifically lead pollution over the past 12 months. Air samples were collected on 7 μm Kapton foils using a nine-stage cascade impactor that separates the particulate matter by aerodynamic size. A 2.2 MeV proton beam impacts the target samples. X-ray intensity versus energy spectra was produced using an Amptek silicon drift detector. Proton-induced x-ray emission (PIXE) techniques were used to analyze the energy spectra and we determined a range of 16 elements present in the aerosol samples including, Al, Si, P, S, Cl, K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Br, and Pb. The elemental composition and concentrations of these elements were determined using GUPIX. Many of the elements suggest airborne soils, however we see trace amounts of lead concentrations only at the minimal level of detection around 1 ng / m3. Preliminary results suggest that lead pollution is not significant however; we believe that the trace amounts of lead detected are due to fuel emissions from small aircraft due to the sampling site near an airport. Extremely fine particles (PM2.5) are found to penetrate deep into the lungs and hence, are found to have harmful health effects on humans. Atmospheric aerosol samples collected in Schenectady, NY were analyzed for evidence for air pollution; specifically lead pollution over the past 12 months. Air samples were collected on 7 μm Kapton foils using a nine-stage cascade impactor that separates the particulate matter by aerodynamic size. A 2.2 MeV proton beam impacts the target samples. X-ray intensity versus energy spectra was produced using an Amptek silicon drift detector. Proton-induced x-ray emission (PIXE) techniques were used to analyze the energy spectra and we determined a range of 16 elements present in

  17. The Tumor Antigen NY-ESO-1 Mediates Direct Recognition of Melanoma Cells by CD4+ T Cells after Intercellular Antigen Transfer

    PubMed Central

    Fonteneau, Jean Francois; Brilot, Fabienne; Münz, Christian

    2016-01-01

    NY-ESO-1–specific CD4+ T cells are of interest for immune therapy against tumors, because it has been shown that their transfer into a patient with melanoma resulted in tumor regression. Therefore, we investigated how NY-ESO-1 is processed onto MHC class II molecules for direct CD4+ T cell recognition of melanoma cells. We could rule out proteasome and autophagy-dependent endogenous Ag processing for MHC class II presentation. In contrast, intercellular Ag transfer, followed by classical MHC class II Ag processing via endocytosis, sensitized neighboring melanoma cells for CD4+ T cell recognition. However, macroautophagy targeting of NY-ESO-1 enhanced MHC class II presentation. Therefore, both elevated NY-ESO-1 release and macroautophagy targeting could improve melanoma cell recognition by CD4+ T cells and should be explored during immunotherapy of melanoma. PMID:26608910

  18. The Tumor Antigen NY-ESO-1 Mediates Direct Recognition of Melanoma Cells by CD4+ T Cells after Intercellular Antigen Transfer.

    PubMed

    Fonteneau, Jean Francois; Brilot, Fabienne; Münz, Christian; Gannagé, Monique

    2016-01-01

    NY-ESO-1-specific CD4(+) T cells are of interest for immune therapy against tumors, because it has been shown that their transfer into a patient with melanoma resulted in tumor regression. Therefore, we investigated how NY-ESO-1 is processed onto MHC class II molecules for direct CD4(+) T cell recognition of melanoma cells. We could rule out proteasome and autophagy-dependent endogenous Ag processing for MHC class II presentation. In contrast, intercellular Ag transfer, followed by classical MHC class II Ag processing via endocytosis, sensitized neighboring melanoma cells for CD4(+) T cell recognition. However, macroautophagy targeting of NY-ESO-1 enhanced MHC class II presentation. Therefore, both elevated NY-ESO-1 release and macroautophagy targeting could improve melanoma cell recognition by CD4(+) T cells and should be explored during immunotherapy of melanoma.

  19. Blockade of CTLA-4 promotes the development of effector CD8+ T lymphocytes and the therapeutic effect of vaccination with an attenuated protozoan expressing NY-ESO-1.

    PubMed

    Dos Santos, Luara Isabela; Galvão-Filho, Bruno; de Faria, Paula Cristina; Junqueira, Caroline; Dutra, Miriam Santos; Teixeira, Santuza Maria Ribeiro; Rodrigues, Maurício Martins; Ritter, Gerd; Bannard, Oliver; Fearon, Douglas Thomas; Antonelli, Lis Ribeiro; Gazzinelli, Ricardo Tostes

    2015-03-01

    The development of cancer immunotherapy has long been a challenge. Here, we report that prophylactic vaccination with a highly attenuated Trypanosoma cruzi strain expressing NY-ESO-1 (CL-14-NY-ESO-1) induces both effector memory and effector CD8(+) T lymphocytes that efficiently prevent tumor development. However, the therapeutic effect of such a vaccine is limited. We also demonstrate that blockade of Cytotoxic T Lymphocyte Antigen 4 (CTLA-4) during vaccination enhances the frequency of NY-ESO-1-specific effector CD8(+) T cells producing IFN-γ and promotes lymphocyte migration to the tumor infiltrate. As a result, therapy with CL-14-NY-ESO-1 together with anti-CTLA-4 is highly effective in controlling the development of an established melanoma.

  20. Tumor-reactive CD8+ T-cell responses after vaccination with NY-ESO-1 peptide, CpG 7909 and Montanide ISA-51: association with survival.

    PubMed

    Karbach, Julia; Gnjatic, Sacha; Bender, Armin; Neumann, Antje; Weidmann, Eckhart; Yuan, Jianda; Ferrara, Cathy A; Hoffmann, Eric; Old, Lloyd J; Altorki, Nasser K; Jäger, Elke

    2010-02-15

    Peptide-based vaccines have led to the induction of antigen-specific CD8(+) T-cell responses in patients with NY-ESO-1 positive cancers. However, vaccine-induced T-cell responses did not generally correlate with improved survival. Therefore, we tested whether a synthetic CpG 7909 ODN (deoxycytidyl-deoxyguanosin oligodeoxy-nucleotides) mixed with NY-ESO-1 peptide p157-165 and incomplete Freund's adjuvants (Montanide(R) ISA-51) led to enhanced NY-ESO-1 antigen-specific CD8(+) immune responses in patients with NY-ESO-1 or LAGE-1 expressing tumors. Of 14 HLA-A2+ patients enrolled in the study, 5 patients withdrew prematurely because of progressive disease and 9 patients completed 1 cycle of immunization. Nine of 14 patients developed measurable and sustained antigen-specific CD8(+) T-cell responses: Four had detectable CD8+ T-cells against NY-ESO-1 after only 2 vaccinations, whereas 5 patients showed a late-onset but durable induction of NY-ESO-1 p157-165 specific T-cell response during continued vaccination after 4 months. In 6 patients, vaccine-induced antigen-specific T-cells became detectable ex vivo and reached frequencies of up to 0.16 % of all circulating CD8(+) T-cells. Postvaccine T-cell clones were shown to recognize and lyse NY-ESO-1 expressing tumor cell lines in vitro. In 6 of 9 patients developing NY-ESO-1-specific immune responses, a favorable clinical outcome with overall survival times of 43+, 42+, 42+, 39+, 36+ and 27+ months, respectively, was observed.

  1. The 'first' instead of the 'oldest'. St. John's Riverside, in Yonkers, N.Y., markets its history.

    PubMed

    Botvin, Judith D

    2002-01-01

    St. John's Riverside Hospital, Yonkers, N.Y., built an integrated campaign upon its long history. Using billboards, print ads, bus shelters, radio and TV it touted its 130-plus years and its long history of firsts. Its integrated campaign proclaimed these "firsts." It specifically targeted its marketing to middle to upper-middle income residents of the surrounding area, particularly females between ages 25 and 54.

  2. The 'first' instead of the 'oldest'. St. John's Riverside, in Yonkers, N.Y., markets its history.

    PubMed

    Botvin, Judith D

    2002-01-01

    St. John's Riverside Hospital, Yonkers, N.Y., built an integrated campaign upon its long history. Using billboards, print ads, bus shelters, radio and TV it touted its 130-plus years and its long history of firsts. Its integrated campaign proclaimed these "firsts." It specifically targeted its marketing to middle to upper-middle income residents of the surrounding area, particularly females between ages 25 and 54. PMID:12238239

  3. Surface radiation climatology for Ny-Ålesund, Svalbard (78.9° N), basic observations for trend detection

    NASA Astrophysics Data System (ADS)

    Maturilli, Marion; Herber, Andreas; König-Langlo, Gert

    2015-04-01

    At Ny-Ålesund (78.9° N), Svalbard, surface radiation measurements of up- and downward short- and longwave radiation are operated since August 1992 in the frame of the Baseline Surface Radiation Network (BSRN), complemented with surface and upper air meteorology since August 1993. The long-term observations are the base for a climatological presentation of the surface radiation data. Over the 21-year observation period, ongoing changes in the Arctic climate system are reflected. Particularly, the observations indicate a strong seasonality of surface warming and related changes in different radiation parameters. The annual mean temperature at Ny-Ålesund has risen by +1.3 ± 0.7 K per decade, with a maximum seasonal increase during the winter months of +3.1 ± 2.6 K per decade. At the same time, winter is also the season with the largest long-term changes in radiation, featuring an increase of +15.6 ± 11.6 Wm-2 per decade in the downward longwave radiation. Furthermore, changes in the reflected solar radiation during the months of snow melt indicate an earlier onset of the warm season by about 1 week compared to the beginning of the observations. The supplementary dataset of Ny-Ålesund surface radiation measurements (available at http://dx.doi.org/10.1594/PANGAEA.150000) provides a valuable data source for the validation of satellite instruments and climate models.

  4. Contrasting groundwater quality in areas with and without gas production by hydraulic fracturing near the PA/NY border

    NASA Astrophysics Data System (ADS)

    Stute, M.; Yan, B.; Ross, J. M.; Chillrud, S. N.; Saberi, P.; Panettieri, R. A.

    2013-12-01

    Shale gas development, including drilling and hydraulic fracturing, is rapidly increasing throughout the United States and, indeed, the rest of the world. Systematic surveys of water quality both pre- and post drilling/production are sparse. To examine the impacts of shale gas production on water quality, pilot studies are being conducted in adjacent counties of western NY (Chemung, Tioga, Broome, and Delaware) and northern PA (Bradford, Susquehanna, and Tioga). These 7 counties along the border of NY and PA share similar geology and demographic compositions and have been identified as a key area to develop shale gas with the key difference that active fracking is occurring in PA but there is no fracking yet in NY due to the current moratorium in that state. Measurements include a suite of volatile organic compounds (VOCs), major and trace elements, methane and its stable isotopes, noble gases and tritium for dating purposes, and the primary radioactive elements of potential concern, radon and radium. We found elevated methane levels on both sides of the border, and some wells show elemental fingerprints characteristic for shale fluids. Field observations at several wells near drill sites in PA suggested elevated levels of organics, possibly from hydraulic fracturing activities. The full suite of lab analyses is currently ongoing and can be used to further characterize sources of these organics.

  5. Immunohistochemical expression of tumor antigens MAGE-A3/4 and NY-ESO-1 in renal oncocytoma and chromophobe renal cell carcinoma.

    PubMed

    Demirović, Alma; Džombeta, Tihana; Tomas, Davor; Spajić, Borislav; Pavić, Ivana; Hudolin, Tvrtko; Milošević, Milan; Cupić, Hrvoje; Krušlin, Božo

    2010-10-15

    The distinction between renal oncocytoma (RO) and chromophobe renal cell carcinoma (ChRCC), especially the eosinophilic variant, can often be difficult. Our study has documented for the first time the expression of MAGE-A3/4 and NY-ESO-1 cancer testis antigens (CTAs) in these tumors. A total of 35 patients (17 ROs and 18 ChRCCs) were included in the study. Two antibodies were used for immunohistochemical staining: 57B recognizing multiple MAGE-A and D8.38 recognizing NY-ESO-1 CTAs. Fifteen (88.2%) samples of RO stained positively for both MAGE-A3/4 and NY-ESO-1 antigens. Regarding ChRCC, seven (38.9%) stained positively for MAGE-A3/4 and six (33.3%) for NY-ESO-1 antigens. Median MAGE-A3/4 expression was moderately positive in RO and negative in ChRCC. The difference in MAGE-A3/4 expression between two tumor groups was significant (P=0.0013). Median NY-ESO-1 expression was strongly positive in RO and negative in ChRCC. The difference in NY-ESO-1 expression between two tumor groups was also significant (P=0.0008). Our study has shown that RO had a significantly higher expression of both CTAs. However, additional research is needed to clarify their potential diagnostic implications.

  6. Centrosomal localisation of the cancer/testis (CT) antigens NY-ESO-1 and MAGE-C1 is regulated by proteasome activity in tumour cells.

    PubMed

    Pagotto, Anna; Caballero, Otavia L; Volkmar, Norbert; Devalle, Sylvie; Simpson, Andrew J G; Lu, Xin; Christianson, John C

    2013-01-01

    The Cancer/Testis (CT) antigen family of genes are transcriptionally repressed in most human tissues but are atypically re-expressed in many malignant tumour types. Their restricted expression profile makes CT antigens ideal targets for cancer immunotherapy. As little is known about whether CT antigens may be regulated by post-translational processing, we investigated the mechanisms governing degradation of NY-ESO-1 and MAGE-C1 in selected cancer cell lines. Inhibitors of proteasome-mediated degradation induced the partitioning of NY-ESO-1 and MAGE-C1 into a detergent insoluble fraction. Moreover, this treatment also resulted in increased localisation of NY-ESO-1 and MAGE-C1 at the centrosome. Despite their interaction, relocation of either NY-ESO-1 or MAGE-C1 to the centrosome could occur independently of each other. Using a series of truncated fragments, the regions corresponding to NY-ESO-1(91-150) and MAGE-C1(900-1116) were established as important for controlling both stability and localisation of these CT antigens. Our findings demonstrate that the steady state levels of NY-ESO-1 and MAGE-C1 are regulated by proteasomal degradation and that both behave as aggregation-prone proteins upon accumulation. With proteasome inhibitors being increasingly used as front-line treatment in cancer, these data raise issues about CT antigen processing for antigenic presentation and therefore immunogenicity in cancer patients.

  7. Atmospheric methane at Zeppelin Station in Ny-Alesund: presentation and analysis of in situ measurements.

    PubMed

    Pedersen, Ine-Therese; Holmen, Kim; Hermansen, Ove

    2005-05-01

    In situ methane (CH(4)) measurement techniques and data from the Zeppelin Station in Ny-Alesund on Svalbard (N 78 degrees 54' E 11 degrees 53') on Mt. Zeppelin (475 m.a.s.l) are presented. The data span the time period from October 1998 to December 2003, though not continuously. The daily mean was calculated from 96 samples per day which are analysed by gas chromatography with flame ionisation detection. Details of the experimental methods and procedures are given. A harmonic function is fitted to the data with a constant trend and seasonal amplitude. The data are also presented in a regression plot showing the difference between the in situ measurements and flask measurements from the National Oceanic and Atmospheric Administration of the Climate Monitoring and Diagnostics Laboratory (NOAA/CMDL) in Boulder, Colorado (http://www.cmdl.noaa.gov/). The Zeppelin data show typical Arctic air characteristics with wintertime pollution episodes from Europe and Russia and a relatively calm summer state. PMID:15877171

  8. Hudson River Paleoclimate, Sea Level, and Human Impact: A Record From Piermont Marsh, NY

    NASA Technical Reports Server (NTRS)

    Kurdyla; Peteet, Dorothy; Liberman, Louisa; Sugar; Wong; Hansen, James E. (Technical Monitor)

    2001-01-01

    A 13.77 meter sediment core from Piermont Marsh, NY (40 00 N, 73 55W) records the local and regional vegetational and foraminiferal history of the Hudson Estuary. The sediments were sampled every 4 cm, which represents a decadal to centuryscale resolution. Basal sediment dating is in progress, and the 11-m depth represents about 4000 years. Changes in plant macrofossils and charcoal appear to indicate differences in salinIty and drought, suggesting changes in climate. Scirpus, Salicornia, and high levels of charcoal seem to indicate drier/more saline conditions, while lack of these macrofossils and increases in Chara/Nitella, aquatic leaves, and very little charcoal suggests wetter conditions. Other macrofossils include Carex, Juncus, Polygonum, Zanichellia, Ruppia. High resolution AMS dating of plant macrofossils is in progress, and will be compared with changes in Hudson River sediment cores offshore. Foraminiferal assemblages from key intervals of the core will be presented. Human impact in the upper sediments is visible from the influx of grass seeds, primarily Phragmites, and the ragweed pollen rise.

  9. American Fisheries Society 136th Annual Meeting Lake Placid, NY 10-14 September, 2006

    USGS Publications Warehouse

    Einhouse, D.; Walsh, M.G.; Keeler, S.; Long, J.M.

    2005-01-01

    The New York Chapter of the American Fisheries Society and the New York State Department of Environmental Conservation invite you to experience the beauty of New York's famous Adirondack Park as the American Fisheries Society (AFS) convenes its 136th Annual Meeting in the legendary Olympic Village of Lake Placid, NY, 10-14 September 2006. Our meeting theme "Fish in the Balance" will explore the interrelation between fish, aquatic habitats, and man, highlighting the challenges facing aquatic resource professionals and the methods that have been employed to resolve conflicts between those that use or have an interest in our aquatic resources. As fragile as it is beautiful, the Adirondack Region is the perfect location to explore this theme. Bordered by Mirror Lake and its namesake, Lake Placid, the Village of Lake Placid has small town charm, but all of the conveniences that a big city would provide. Whether its reliving the magic of the 1980 hockey team's "Miracle on Ice" at the Lake Placid Olympic Center, getting a panoramic view of the Adirondack high peaks from the top of the 90 meter ski jumps, fishing or kayaking in adjacent Mirror Lake, hiking a mountain trail, or enjoying a quiet dinner or shopping excursion in the various shops and restaurants that line Main Street, Lake Placid has something for everyone.

  10. Atmospheric methane at Zeppelin Station in Ny-Alesund: presentation and analysis of in situ measurements.

    PubMed

    Pedersen, Ine-Therese; Holmen, Kim; Hermansen, Ove

    2005-05-01

    In situ methane (CH(4)) measurement techniques and data from the Zeppelin Station in Ny-Alesund on Svalbard (N 78 degrees 54' E 11 degrees 53') on Mt. Zeppelin (475 m.a.s.l) are presented. The data span the time period from October 1998 to December 2003, though not continuously. The daily mean was calculated from 96 samples per day which are analysed by gas chromatography with flame ionisation detection. Details of the experimental methods and procedures are given. A harmonic function is fitted to the data with a constant trend and seasonal amplitude. The data are also presented in a regression plot showing the difference between the in situ measurements and flask measurements from the National Oceanic and Atmospheric Administration of the Climate Monitoring and Diagnostics Laboratory (NOAA/CMDL) in Boulder, Colorado (http://www.cmdl.noaa.gov/). The Zeppelin data show typical Arctic air characteristics with wintertime pollution episodes from Europe and Russia and a relatively calm summer state.

  11. Assessment and Molecular Characterization of Human Intestinal Parasites in Bivalves from Orchard Beach, NY, USA

    PubMed Central

    Tei, Freda F.; Kowalyk, Steven; Reid, Jhenelle A.; Presta, Matthew A.; Yesudas, Rekha; Mayer, D.C. Ghislaine

    2016-01-01

    Bivalves have been shown to be carriers of the human intestinal parasites Cryptosporidium parvum and Toxoplasma gondii. The goal of this study is to determine the prevalence of protozoan parasites in mollusks of New York City using a polymerase chain reaction (PCR)-based assay. Four species of mollusks, Mya arenaria, Geukensia demissa, Crassostrea virginica, and Mytilis edulis, were collected from Orchard Beach, NY in the fall of 2014, totaling 159 specimens. Each individual mollusk was dissected to harvest the digestive gland, the mantle, the gills, the foot and the siphon. The tissues were assayed for the presence of Cryptosporidium parvum, Giardia lamblia, and Toxoplasma gondii DNA by using primers that target parasite-specific genes. C. parvum was found at a prevalence of 50%, 11.3%, and 1%, respectively, in Mya arenaria, G. demissa, and Mytilis edulis. C. parvum DNA was detected in all the tissues of these bivalve species, except the gills. Furthermore, G. lamblia was detected in Mya arenaria, G. demissa, Crassostrea virginica and Mytilis edulis at a prevalence of 37.5%, 4.5%, 60%, and 20.6%, respectively, while T. gondii DNA was not detected. PMID:27043590

  12. Modeling a High Resolution Stream Chloride Time Series from the Biscuit Brook Catchment, Catskills, NY

    NASA Astrophysics Data System (ADS)

    Shaw, S. B.; Walter, M. T.; Harpold, A. A.

    2006-12-01

    Compelling work by Kirchner and colleagues (e.g., Kirchner et al., 2001, J. of Hydro., 254:81-100) suggests that lengthy, high resolution chloride time series can reveal the underlying travel time distribution of water in a catchment. However, few data sets of suitable length or sampling frequency have been available for assessment. Our analysis of a ten-year, weekly stream chloride time series data set for the steep, forested Biscuit Brook catchment in the Catskill Mountains of NY resulted in a 1/f spectral density plot. We used a lumped, two reservoir model with an upper reservoir representing quickflow and a lower reservoir representing base flow to reasonably replicate stream flow and stream chloride concentrations. A spectral analysis of the modeled daily chloride signal also resulted in a 1/f spectral density plot. This suggests that in some systems a 1/f signature can result from processes other than superposition of lateral chemical transport pathways, as has been previously hypothesized by other researchers. In this case, it appears that a long-term periodicity established by seasonal moisture deficits combines with shorter wavelength variations from rainfall inputs to create a 1/f spectral density for chloride concentration.

  13. Water residence times and nutrient budgets across an urbanizing gradient (Croton water supply area, NY)

    NASA Astrophysics Data System (ADS)

    Vitvar, T.; Burns, D. A.; Duncan, J. M.; Hassett, J. M.; Mitchell, M. J.

    2002-05-01

    Water residence times and nutrient budgets in 3 small watersheds in the Croton water supply area, NY, were examined. The watersheds (less than 1km 2) have different level of urbanization (natural, semi-developed and fully developed), different mechanisms of runoff generation (quick flow on roads and slow flow through subsurface) and different watershed landscape characteristics (wet zones, hillslopes) . Measurements of the comprehensive chemical suite incl. components of nitrogen budget in the throughfall, stream water, soil water and groundwater in the saturated zone were performed bi-weekly over a period up to 2 years. Mean water residence times of the stream water were estimated using Oxygen-18 and Helium-3/Tritium isotopes. There are significant differences in the chemical composition and decrease of nitrification intensity and of mean streamwater residence time along the increasing watershed development. Within each watershed, longer water residence times (up to over 2 years) were estimated in the wetland zones without direct communication with streams in comparison to hillslope areas (up to over 1 year). The results can be used in watershed management and planning of the further urbanization of this water supply area.

  14. Ten-Year Study of the Stringently Defined Otitis-prone Child in Rochester, NY.

    PubMed

    Pichichero, Michael E

    2016-09-01

    This review summarizes a prospective, longitudinal 10-year study in Rochester, NY, with virtually every clinically diagnosed acute otitis media (AOM) confirmed by bacterial culture of middle ear fluid. Children experiencing 3 episodes within 6 months or 4 episodes in 12 months were considered stringently defined otitis prone (sOP). We found stringent diagnosis compared with clinical diagnosis reduced the frequency of children meeting the OP definition from 27% to 6% resulting in 14.8% and 2.4% receiving tympanostomy tubes, respectively. Significantly more often respiratory syncytial virus infection led to AOM in sOP than non-otitis-prone children that correlated with diminished total respiratory syncytial virus-specific serum IgG. sOP children produced low levels of antibody to Streptococcus pneumoniae and Haemophilus influenzae candidate vaccine protein antigens and to routine pediatric vaccines. sOP children generated significantly fewer memory B cells, functional and memory T cells to otopathogens following nasopharyngeal colonization and AOM than non-otitis-prone children and they had defects in antigen-presenting cells. PMID:27273691

  15. Hudson River Paleoclimate, Sea Level, and Human Impact - A record from Piermont Marsh, NY

    NASA Astrophysics Data System (ADS)

    Peteet, D. M.; Peteet, D. M.; Kurdyla, D.; Liberman, L.; Sugar, A.; Wong, J.

    2001-05-01

    A 13.77 meter sediment core from Piermont Marsh, NY (41 00 N, 73 55W) records the local and regional vegetational and foraminiferal history of the Hudson Estuary. The sediments were sampled every 4 cm, which represents a decadal to century-scale resolution. Basal sediment dating is in progress, and the ll-m depth represents about 4000 years. Fluctuations in plant macrofossils and charcoal appear to indicate differences in salinlty and drought, suggesting major changes in climate. Scirpus, Salicornia, and high levels of charcoal seem to indicate drier/more saline conditions, while lack of these macrofossils and increases in Chara/Nitella, aquatic leaves, and very little charcoal suggests wetter conditions. Other macrofossils present include Carex, Juncus, Polygonum, Zanichellia, and Ruppia. High resolution AMS dating of plant macrofossils is in progress, and will be compared with sedimentation in Hudson River sediment cores nearby. Foraminiferal asssemblages from key intervals of the core will be presented. Human impact in the upper sediments is visible from the influx of grass seeds, primarily Phragmites, and the ragweed pollen rise.

  16. Madison County, NY, food and paper waste pilot compost program, March 1993

    SciTech Connect

    Zecca, J.A.; Pike, W.R.; Higgins, T.W.

    1995-05-01

    Madison County, NY has a program which promises to extend the life of their landfill, by as much as double, at minimal cost. The County already has an impressive list of successful recycling programs but recognized that there was a need for further action. The objective of the new program is to reduce the size of the stream entering the landfill by composting a source separated fraction of the waste stream. Costs of collection are minimized by co-collecting the separately bagged {open_quotes}clean{close_quotes} household compost fraction with the balance of the trash. This is separated from the balance of the waste on the landfill site and composted in windrows. When the program started, the composted material was targeted for use as landfill cover to minimize the quality requirements. However, testing has shown that the compost is acceptable for all applications. Although the opening phase of the program might properly be called a pilot, it was started with the intention to enlarge it as events indicate such was possible. Expansion to the balance of the county, inclusion of specific commercial waste and inclusion of a broader range of household compostables are currently in progress.

  17. Characterization of a Single Magnetotactic Bacterial Species from Devil's Bathtub, Mendon Ponds Park, Honeoye Falls, NY

    NASA Astrophysics Data System (ADS)

    Wagner, C.; Tarduno, J. A.; Stein, A.; Sia, E.

    2015-12-01

    Magnetotactic bacteria (MTB) belong to a lineage of prokaryotic bacteria that synthesize magnetosomes, single domain magnetic particles (typically magnetite or greigite) with an average size of 50 nanometers. MTB utilize magnetosomes through magnetotaxis, the alignment and movement along magnetic field lines to navigate towards preferred environmental conditions. MTB are sensitive to different environments and are thought to exhibit varying magnetosome morphologies, compositions, sizes, and quantities in regards to the environments which they inhabit. These characteristics allow MTB and magnetofossils (preserved magnetosomes) to be used as modern/paleoenvironmental recorders and biomarkers for environmental change(s). Devil's Bathtub (Mendon Ponds Park, Honeoye Falls, NY) is a meromictic glacial kettle pond surrounded by deciduous tree cover. Here we examine one species of MTB based on prominence of this particular morphology at this locale. Magnetotaxis and morphology of this species have been observed using light microscopy. Micrographs have also been taken using Transmission Electron Microscopy (TEM) to verify cell morphology and to determine magnetosome morphology. TEM and magnetic hysteresis measurements were done to find and test the composition of magnetosomes. In this study we also focus on DNA sequencing and characterization of this MTB, as there are few MTB species which have been DNA sequenced successfully. Data from these experiments are directly applicable to this up-and-coming area of research as it will aid in the understanding and correlation of magnetosome and magnetofossils with environmental characteristics.

  18. Assessment and Molecular Characterization of Human Intestinal Parasites in Bivalves from Orchard Beach, NY, USA.

    PubMed

    Tei, Freda F; Kowalyk, Steven; Reid, Jhenelle A; Presta, Matthew A; Yesudas, Rekha; Mayer, D C Ghislaine

    2016-04-01

    Bivalves have been shown to be carriers of the human intestinal parasites Cryptosporidium parvum and Toxoplasma gondii. The goal of this study is to determine the prevalence of protozoan parasites in mollusks of New York City using a polymerase chain reaction (PCR)-based assay. Four species of mollusks, Mya arenaria, Geukensia demissa, Crassostrea virginica, and Mytilis edulis, were collected from Orchard Beach, NY in the fall of 2014, totaling 159 specimens. Each individual mollusk was dissected to harvest the digestive gland, the mantle, the gills, the foot and the siphon. The tissues were assayed for the presence of Cryptosporidium parvum, Giardia lamblia, and Toxoplasma gondii DNA by using primers that target parasite-specific genes. C. parvum was found at a prevalence of 50%, 11.3%, and 1%, respectively, in Mya arenaria, G. demissa, and Mytilis edulis. C. parvum DNA was detected in all the tissues of these bivalve species, except the gills. Furthermore, G. lamblia was detected in Mya arenaria, G. demissa, Crassostrea virginica and Mytilis edulis at a prevalence of 37.5%, 4.5%, 60%, and 20.6%, respectively, while T. gondii DNA was not detected. PMID:27043590

  19. Highly Polar Organic Compounds in Summer Cloud Water from Whiteface Mountain, NY

    NASA Astrophysics Data System (ADS)

    Sagona, J. A.; Dukett, J. E.; Mazurek, M.

    2010-12-01

    Highly polar organic compounds (HPOC) containing multiple oxygen atoms are of interest due to the aerosol direct and indirect effects and uncertainty they may contribute to climate forcing. Atmospheric HPOC exist as particulate or dissolved chemical species depending on relative humidity. HPOC solid mixtures are thought to scatter and absorb light due to particle size and color (white to yellow). Understanding the chemical composition of HPOC in cloud water (CW) is important for modeling and predicting the direct and indirect influences of this organic aerosol component on the climate system. In this initial study we present a detailed molecular examination of 10 CW samples. CW samples were collected in September 2009 using an automated CW collector at Whiteface Mountain (elevation 1483 m) in the Adirondack Mountains in upstate NY. We performed QA/QC experiments for the CW samples and analyzed the HPOC mixtures by ultratrace gas chromatography/mass spectrometry. Diacids such as oxalic acid and succinic acid were major components of the CW HPOC with concentrations on the order of 15 ng/mL. Substituted diacids, monoacids, and sugars also were present. We discuss the possible sources of these compounds in light of back-trajectory analysis.

  20. An Adaptation of Constructive Alternativism as Theory for Audience Analysis.

    ERIC Educational Resources Information Center

    Losee, Doug

    An examination of audience analysis factors in representative communication textbooks indicates that current pedagogical applications are broadly categorized into demographic and dispositional properties. The adaptation of constructivist theory to audience analysis can give speech students reasons why the speaker-audience relationship is as it is.…

  1. MAGE-A is More Highly Expressed Than NY-ESO-1 in a Systematic Immunohistochemical Analysis of 3668 Cases.

    PubMed

    Kerkar, Sid P; Wang, Zeng-Feng; Lasota, Jerzy; Park, Tristen; Patel, Krishna; Groh, Eric; Rosenberg, Steven A; Miettinen, Markku M

    2016-05-01

    Two cancer testis antigens, the New York esophageal squamous cell carcinoma-1 (NY-ESO-1) and the melanoma-antigen family A (MAGE-A), represent promising immunotherapy targets due to the low expression of these antigens in nonmalignant tissue. To assess overexpression patterns in various cancers, we performed a systematic immunohistochemical analysis for NY-ESO-1 and MAGE-A on tissue array samples of 3668 common epithelial carcinomas (CA) and germ cell tumors of high prevalence and mortality. Here, we find significantly higher expression of MAGE-A (>50% on tumor cells) compared with NY-ESO-1 in several CAs including cutaneous squamous cell carcinomas (SCC) (52.8%/2.8%), esophageal SCC (50%/0%), head and neck SCC (41.1%/<1%), bladder urothelial CA (40.4%/8.3%), cervical/anal SCC (37.5%/0%), lung SCC (34%/3.8%), lung adenocarcinomas (27.6%/3.9%), ovarian CA (26.4%/3.6%), endometrial CA (26.3%/1.3%), lung small cell CA (24.4%/2.4%), gastric adenocarcinomas (20%/4%), breast mucinous CA (19.3%/0%), hepatocellular CA (18.8%/1.2%), breast infiltrating ductal CA (16.4%/1.8%), colorectal adenocarcinomas (10.7%/<1%), cholangiocarcinomas (9.8%/0%), thymic CA (9%/4.5%), and mesotheliomas (7.9%/<1%). Furthermore, high expression of MAGE-A, but not NY-ESO-1, was seen in whole slide evaluations of an independent cohort of metastatic SCC (45.5%/3.6%) and metastatic CA (13.5%/0%) of various primaries with significantly higher expression of MAGE-A in metastatic SCC compared with other metastatic CA. MAGE-A is also more highly expressed in germ cell tumors, seminomas (69%/3.5%) and nonseminomas (40.1%/4.7%). In summary, MAGE-A is more highly expressed than NY-ESO-1 in a majority of human malignancies, and targeting MAGE-A may benefit a large number of patients.

  2. Distribution and prognostic relevance of tumor-infiltrating lymphocytes (TILs) and PD-1/PD-L1 immune checkpoints in human brain metastases.

    PubMed

    Harter, Patrick N; Bernatz, Simon; Scholz, Alexander; Zeiner, Pia S; Zinke, Jenny; Kiyose, Makoto; Blasel, Stella; Beschorner, Rudi; Senft, Christian; Bender, Benjamin; Ronellenfitsch, Michael W; Wikman, Harriet; Glatzel, Markus; Meinhardt, Matthias; Juratli, Tareq A; Steinbach, Joachim P; Plate, Karl H; Wischhusen, Jörg; Weide, Benjamin; Mittelbronn, Michel

    2015-12-01

    The activation of immune cells by targeting checkpoint inhibitors showed promising results with increased patient survival in distinct primary cancers. Since only limited data exist for human brain metastases, we aimed at characterizing tumor infiltrating lymphocytes (TILs) and expression of immune checkpoints in the respective tumors. Two brain metastases cohorts, a mixed entity cohort (n = 252) and a breast carcinoma validation cohort (n = 96) were analyzed for CD3+, CD8+, FOXP3+, PD-1+ lymphocytes and PD-L1+ tumor cells by immunohistochemistry. Analyses for association with clinico-epidemiological and neuroradiological parameters such as patient survival or tumor size were performed. TILs infiltrated brain metastases in three different patterns (stromal, peritumoral, diffuse). While carcinomas often show a strong stromal infiltration, TILs in melanomas often diffusely infiltrate the tumors. Highest levels of CD3+ and CD8+ lymphocytes were seen in renal cell carcinomas (RCC) and strongest PD-1 levels on RCCs and melanomas. High amounts of TILs, high ratios of PD-1+/CD8+ cells and high levels of PD-L1 were negatively correlated with brain metastases size, indicating that in smaller brain metastases CD8+ immune response might get blocked. PD-L1 expression strongly correlated with TILs and FOXP3 expression. No significant association of patient survival with TILs was observed, while high levels of PD-L1 showed a strong trend towards better survival in melanoma brain metastases (Log-Rank p = 0.0537). In summary, melanomas and RCCs seem to be the most immunogenic entities. Differences in immunotherapeutic response between tumor entities regarding brain metastases might be attributable to this finding and need further investigation in larger patient cohorts.

  3. Distribution and prognostic relevance of tumor-infiltrating lymphocytes (TILs) and PD-1/PD-L1 immune checkpoints in human brain metastases.

    PubMed

    Harter, Patrick N; Bernatz, Simon; Scholz, Alexander; Zeiner, Pia S; Zinke, Jenny; Kiyose, Makoto; Blasel, Stella; Beschorner, Rudi; Senft, Christian; Bender, Benjamin; Ronellenfitsch, Michael W; Wikman, Harriet; Glatzel, Markus; Meinhardt, Matthias; Juratli, Tareq A; Steinbach, Joachim P; Plate, Karl H; Wischhusen, Jörg; Weide, Benjamin; Mittelbronn, Michel

    2015-12-01

    The activation of immune cells by targeting checkpoint inhibitors showed promising results with increased patient survival in distinct primary cancers. Since only limited data exist for human brain metastases, we aimed at characterizing tumor infiltrating lymphocytes (TILs) and expression of immune checkpoints in the respective tumors. Two brain metastases cohorts, a mixed entity cohort (n = 252) and a breast carcinoma validation cohort (n = 96) were analyzed for CD3+, CD8+, FOXP3+, PD-1+ lymphocytes and PD-L1+ tumor cells by immunohistochemistry. Analyses for association with clinico-epidemiological and neuroradiological parameters such as patient survival or tumor size were performed. TILs infiltrated brain metastases in three different patterns (stromal, peritumoral, diffuse). While carcinomas often show a strong stromal infiltration, TILs in melanomas often diffusely infiltrate the tumors. Highest levels of CD3+ and CD8+ lymphocytes were seen in renal cell carcinomas (RCC) and strongest PD-1 levels on RCCs and melanomas. High amounts of TILs, high ratios of PD-1+/CD8+ cells and high levels of PD-L1 were negatively correlated with brain metastases size, indicating that in smaller brain metastases CD8+ immune response might get blocked. PD-L1 expression strongly correlated with TILs and FOXP3 expression. No significant association of patient survival with TILs was observed, while high levels of PD-L1 showed a strong trend towards better survival in melanoma brain metastases (Log-Rank p = 0.0537). In summary, melanomas and RCCs seem to be the most immunogenic entities. Differences in immunotherapeutic response between tumor entities regarding brain metastases might be attributable to this finding and need further investigation in larger patient cohorts. PMID:26517811

  4. Whole-lake nitrate addition for control of methylmercury in mercury-contaminated Onondaga Lake, NY.

    PubMed

    Matthews, David A; Babcock, David B; Nolan, John G; Prestigiacomo, Anthony R; Effler, Steven W; Driscoll, Charles T; Todorova, Svetoslava G; Kuhr, Kenneth M

    2013-08-01

    Methylmercury (MeHg) strongly bioaccumulates in aquatic food webs resulting in exposure to humans and wildlife through consumption of fish. Production of MeHg is promoted by anaerobic conditions and the supply of inorganic Hg (Hg(2+)), sulfate (SO4(2-)), and labile organic carbon. The anaerobic sediments of stratified lakes are particularly active zones for methylation of Hg(2+) and can be an important source of MeHg to the water column during summer anoxia and fall turnover. Nitrate (NO3(-)) addition has recently been proposed as a novel approach for the control of MeHg accumulation in the hypolimnia of Hg-contaminated lakes. In 2011, a whole-lake NO3(-) addition pilot test was conducted in Hg-contaminated Onondaga Lake, NY with the objective of limiting release of MeHg from the pelagic sediments to the hypolimnion through maintenance of NO3(-)-N concentrations >1mgN/L. A liquid calcium-nitrate solution was added to the hypolimnion as a neutrally buoyant plume approximately three times per week during the summer stratification interval. Maximum hypolimnetic concentrations of MeHg and soluble reactive phosphorus (SRP) decreased 94% and 95% from 2009 levels, suggesting increased sorption to Fe and Mn oxyhydroxides in surficial sediments as the regulating mechanism. Increased MeHg concentrations in the upper waters during fall turnover, which had been a generally recurring pattern, did not occur in 2011, resulting in decreased exposure of aquatic organisms to MeHg. Over the 1992-2011 interval, the hypolimnetic NO3(-) supply explained 85% and 95% of the interannual variations in hypolimnetic accumulations of SRP and MeHg, respectively.

  5. Hydrometeorological and climatological conditions associated with flash flooding in the Catskill Mountains, NY

    NASA Astrophysics Data System (ADS)

    Teale, N. G.; Quiring, S. M.

    2014-12-01

    Flash flooding is a concern in watersheds of the New York City Water Supply System, as the turbidity associated with rapid flooding is unacceptable in an unfiltered water supply. Previous studies suggest that flash flooding will occur more frequently in this region in a changing climate. Therefore, a thorough understanding of the conditions associated with flash flooding are important for effective watershed management. Seven flash floods were identified in the hydrologic record for the Neversink River near Claryville, NY from 1 April 1987 through 15 July 2014. Case studies using Weather Prediction Center forecast maps, National Centers for Environmental Prediction/ National Center for Atmospheric Research (NCEP/NCAR) reanalysis daily composites, and co-operative station data were used to characterize each event. Forecast maps indicate synoptic-scale frontal activity concurrent with all flash flood events. The four winter flash flood peaks are associated with rain on existing snowpack, with anomalously warm 1000 mb temperatures and anomalously high precipitation rates. The three summer flash flood peaks are associated with convective activity, high precipitation rates, anomalously warm 1000 mb temperatures, and southerly winds. NCEP/NCAR climate composites for winter and summer flash flood events are consistent with the case studies presented. The frequency of these broad-scale conditions suggest that localized effects of the basin conditions separate flash flood events from other high discharge events. Recognizing these conditions in the context of climate predictions is useful for effective and proactive water management in the region to maintain an unfiltered water supply for the greater New York City area.

  6. Local Volume TiNy Titans: gaseous dwarf-dwarf interactions in the Local Universe

    NASA Astrophysics Data System (ADS)

    Pearson, Sarah; Besla, Gurtina; Putman, Mary E.; Lutz, Katharina A.; Fernández, Ximena; Stierwalt, Sabrina; Patton, David R.; Kim, Jinhyub; Kallivayalil, Nitya; Johnson, Kelsey; Sung, Eon-Chang

    2016-06-01

    In this paper, we introduce the Local Volume TiNy Titans sample (LV-TNT), which is a part of a larger body of work on interacting dwarf galaxies: TNT . This LV-TNT sample consists of 10 dwarf galaxy pairs in the Local Universe (<30 Mpc from Milky Way), which span mass ratios of M*, 1/M*, 2 < 20, projected separations <100 kpc, and pair member masses of log(M*/M⊙) < 9.9. All 10 LV-TNT pairs have resolved synthesis maps of their neutral hydrogen, are located in a range of environments and captured at various interaction stages. This enables us to do a comparative study of the diffuse gas in dwarf-dwarf interactions and disentangle the gas lost due to interactions with haloes of massive galaxies, from the gas lost due to mutual interaction between the dwarfs. We find that the neutral gas is extended in the interacting pairs when compared to non-paired analogues, indicating that gas is tidally pre-processed. Additionally, we find that the environment can shape the H I distributions in the form of trailing tails and that the gas is not unbound and lost to the surroundings unless the dwarf pair is residing near a massive galaxy. We conclude that a nearby, massive host galaxy is what ultimately prevents the gas from being re-accreted. Dwarf-dwarf interactions thus represent an important part of the baryon cycle of low-mass galaxies, enabling the `parking' of gas at large distances to serve as a continual gas supply channel until accretion by a more massive host.

  7. Missing-Presumed Eroded: Holocene Sedimentation Patterns in Seneca Lake, NY

    NASA Astrophysics Data System (ADS)

    Loddengaard, K.; Curtin, T. M.

    2006-12-01

    The Finger Lakes of New York underwent a significant change in climate during the transition from the Hypsithermal to the Neoglacial at ~5 ka. To determine the effects of changes in climate on sedimentation patterns in the northern region of Seneca Lake, NY, we analyzed three cores that were collected along a shallow to deep water transect. Analyses of magnetic susceptibility, organic matter and carbonate content, fossil content, and grain size at a 1-cm interval were used to correlate cores, identify periods of erosion or non- deposition and assess the relative truncation of the Holocene sediment record. The stratigraphy and sedimentology of the cores indicate the lake contains a spatially and temporally discontinuous sediment record. Preliminary results indicate that the early to mid-Holocene sediment record was preferentially eroded in the deepwater cores, between 20 and 50 m water depth. At the deepwater sites, there is a gap in the sediment record that spans between ~12 and ~5 ka. Conversely, the late Holocene record is more complete in the deepwater cores than in the shallow water cores (12-20 m water depth). This data suggests a significant change on the controls and location of erosion and deposition in the lake during the Holocene. We hypothesize that the early to mid-Holocene sediment may have been eroded by strong internal seiches during stormy conditions that were initiated by cooler temperatures and a change in the position of the jet stream during the Hypsithermal-Neoglacial transition. Erosion occurred primarily in shallow water sites (<~30 m) during the Neoglacial, perhaps as a result of a change in the number and frequency of storms or strength of the internal seiche.

  8. Microbial Influences on Trace Metal Cycling in a Meromictic Lake, Fayetteville Green Lake, NY

    NASA Astrophysics Data System (ADS)

    Zerkle, A. L.; House, C.; Kump, L.

    2002-12-01

    Microorganisms can exist in aquatic environments at very high cell densities of up to 1011 cells/L, and can accumulate significant quantities of trace metals. Bacteria actively take up bioactive trace metals, including Fe, Zn, Mn, Co, Ni, Cu, and Mo, which function as catalytic centers in metalloproteins and metal-activated enzymes involved in virtually all cellular functions. In addition, bacteria may catalyze the release of trace metals from inorganic substrates by processes such as the reduction of iron and manganese oxides, suggesting that trace metal distributions within a natural environment dominated by microbial processes may be controlled primarily by microbial ecology. Fayetteville Green Lake (FGL), NY, is a permanently stratified meromictic lake that has a well-oxygenated surface water mass (mixolimnion) overlying a relatively stagnant, anoxic deep water mass (monimolimnion). A chemocline separates the water masses at around 20m depth, where oxygen concentrations decrease and sulfate and methane concentrations increase. In addition, previous studies have indicated that trace metals such as V, Cr, Co, Mn, and Fe reach elevated concentrations at the chemocline. Using fluorescent in situ hybridization (FISH) of FGL samples from depths of up to 40m with bacterial and archaeal probes, we have shown that fluctuating redox conditions within the FGL water column correlate with significant variations in the composition and distribution of microbial populations with depth. The mixolimnion is dominated by Eubacteria, with increasing concentrations of Archaea in the lower anoxic zone. Increases in microbial cell densities coincide with increases in trace metals at the chemocline, suggesting microbial activity may be responsible for trace metal release at this boundary. 16S rRNA PCR cloning techniques are currently being used to identify dominant microbial populations at various levels within the FGL water column. Future studies will focus on the potential for these

  9. Efficacy of vaccination with recombinant vaccinia and fowlpox vectors expressing NY-ESO-1 antigen in ovarian cancer and melanoma patients

    PubMed Central

    Odunsi, Kunle; Matsuzaki, Junko; Karbach, Julia; Neumann, Antje; Mhawech-Fauceglia, Paulette; Miller, Austin; Beck, Amy; Morrison, Carl D.; Ritter, Gerd; Godoy, Heidi; Lele, Shashikant; duPont, Nefertiti; Edwards, Robert; Shrikant, Protul; Old, Lloyd J.; Gnjatic, Sacha; Jäger, Elke

    2012-01-01

    Recombinant poxviruses (vaccinia and fowlpox) expressing tumor-associated antigens are currently being evaluated in clinical trials as cancer vaccines to induce tumor-specific immune responses that will improve clinical outcome. To test whether a diversified prime and boost regimen targeting NY-ESO-1 will result in clinical benefit, we conducted two parallel phase II clinical trials of recombinant vaccinia-NY-ESO-1 (rV-NY-ESO-1), followed by booster vaccinations with recombinant fowlpox-NY-ESO-1 (rF-NY-ESO-1) in 25 melanoma and 22 epithelial ovarian cancer (EOC) patients with advanced disease who were at high risk for recurrence/progression. Integrated NY-ESO-1-specific antibody and CD4+ and CD8+ T cells were induced in a high proportion of melanoma and EOC patients. In melanoma patients, objective response rate [complete and partial response (CR+PR)] was 14%, mixed response was 5%, and disease stabilization was 52%, amounting to a clinical benefit rate (CBR) of 72% in melanoma patients. The median PFS in the melanoma patients was 9 mo (range, 0–84 mo) and the median OS was 48 mo (range, 3–106 mo). In EOC patients, the median PFS was 21 mo (95% CI, 16–29 mo), and median OS was 48 mo (CI, not estimable). CD8+ T cells derived from vaccinated patients were shown to lyse NY-ESO-1-expressing tumor targets. These data provide preliminary evidence of clinically meaningful benefit for diversified prime and boost recombinant pox-viral-based vaccines in melanoma and ovarian cancer and support further evaluation of this approach in these patient populations. PMID:22454499

  10. Efficacy of vaccination with recombinant vaccinia and fowlpox vectors expressing NY-ESO-1 antigen in ovarian cancer and melanoma patients.

    PubMed

    Odunsi, Kunle; Matsuzaki, Junko; Karbach, Julia; Neumann, Antje; Mhawech-Fauceglia, Paulette; Miller, Austin; Beck, Amy; Morrison, Carl D; Ritter, Gerd; Godoy, Heidi; Lele, Shashikant; duPont, Nefertiti; Edwards, Robert; Shrikant, Protul; Old, Lloyd J; Gnjatic, Sacha; Jäger, Elke

    2012-04-10

    Recombinant poxviruses (vaccinia and fowlpox) expressing tumor-associated antigens are currently being evaluated in clinical trials as cancer vaccines to induce tumor-specific immune responses that will improve clinical outcome. To test whether a diversified prime and boost regimen targeting NY-ESO-1 will result in clinical benefit, we conducted two parallel phase II clinical trials of recombinant vaccinia-NY-ESO-1 (rV-NY-ESO-1), followed by booster vaccinations with recombinant fowlpox-NY-ESO-1 (rF-NY-ESO-1) in 25 melanoma and 22 epithelial ovarian cancer (EOC) patients with advanced disease who were at high risk for recurrence/progression. Integrated NY-ESO-1-specific antibody and CD4(+) and CD8(+) T cells were induced in a high proportion of melanoma and EOC patients. In melanoma patients, objective response rate [complete and partial response (CR+PR)] was 14%, mixed response was 5%, and disease stabilization was 52%, amounting to a clinical benefit rate (CBR) of 72% in melanoma patients. The median PFS in the melanoma patients was 9 mo (range, 0-84 mo) and the median OS was 48 mo (range, 3-106 mo). In EOC patients, the median PFS was 21 mo (95% CI, 16-29 mo), and median OS was 48 mo (CI, not estimable). CD8(+) T cells derived from vaccinated patients were shown to lyse NY-ESO-1-expressing tumor targets. These data provide preliminary evidence of clinically meaningful benefit for diversified prime and boost recombinant pox-viral-based vaccines in melanoma and ovarian cancer and support further evaluation of this approach in these patient populations.

  11. Arctic warming, moisture increase and circulation changes observed in the Ny-Ålesund homogenized radiosonde record

    NASA Astrophysics Data System (ADS)

    Maturilli, Marion; Kayser, Markus

    2016-07-01

    Radiosonde measurements obtained at the Arctic site Ny-Ålesund (78.9°N, 11.9°E), Svalbard, from 1993 to 2014 have been homogenized accounting for instrumentation discontinuities by correcting known errors in the manufacturer provided profiles. The resulting homogenized radiosonde record is provided as supplementary material at http://doi.pangaea.de/10.1594/PANGAEA.845373. From the homogenized data record, the first Ny-Ålesund upper-air climatology of wind, temperature and humidity is presented, forming the background for the analysis of changes during the 22-year period. Particularly during the winter season, a strong increase in atmospheric temperature and humidity is observed, with a significant warming of the free troposphere in January and February up to 3 K per decade. This winter warming is even more pronounced in the boundary layer below 1 km, presumably amplified by mesoscale processes including e.g. orographic effects or the boundary layer capping inversion. Though the largest contribution to the increasing atmospheric water vapour column in winter originates from the lowermost 2 km, no increase in the contribution by specific humidity inversions is detected. Instead, we find an increase in the humidity content of the large-scale background humidity profiles. At the same time, the tropospheric flow in winter is found to occur less frequent from northerly directions and to the same amount more frequent from the South. We conclude that changes in the atmospheric circulation lead to an enhanced advection of warm and moist air from lower latitudes to the Svalbard region in the winter season, causing the warming and moistening of the atmospheric column above Ny-Ålesund, and link the observations to changes in the Arctic Oscillation.

  12. Porous VOxNy nanoribbons supported on CNTs as efficient and stable non-noble electrocatalysts for the oxygen reduction reaction

    PubMed Central

    Huang, K.; Bi, K.; Lu, Y. K.; Zhang, R.; Liu, J.; Wang, W. J.; Tang, H. L.; Wang, Y. G.; Lei, M.

    2015-01-01

    Novel nanocomposites of carbon nanotubes supported porous VOxNy nonoribbons (VOxNy-CNTs) have been synthesized by the annealing of the sol-gel mixture of CNTs and V2O5 under NH3 atmosphere as well as the ageing process in air. Besides the morphological and structural characterizations revealed by TEM, SEAD, EDS, XRD and XPS measurements, typical electrochemical tests including cyclic voltammetry (CV), rotating disk electrode (RDE) and chronoamperometry have been employed to determine the oxygen reduction reaction (ORR) performance of VOxNy-CNTs. Inspiringly, the results indicate that VOxNy-CNTs catalyst exhibits a 0.4 mA/cm2 larger diffusion-limited current density, a 0.10  V smaller onset potential value, a 10.73% less of ORR current decay and an excellent methanol-tolerance compared with commercial Pt/C catalyst. Therefore, we have reasonable grounds to believe that this new VOxNy-CNTs nanocomposites can be regarded as a promising non-precious methanol-tolerant ORR catalyst candidate for alkaline fuel cells. PMID:26616719

  13. Self-rectifying resistive-switching characteristics with ultralow operating currents in SiOxNy/AlN bilayer devices

    NASA Astrophysics Data System (ADS)

    Kwon, Jeong Yong; Park, Ju Hyun; Kim, Tae Geun

    2015-06-01

    We propose a SiOxNy/AlN bilayer resistive switching random access memory scheme to eliminate crosstalk in a crossbar array structure. We demonstrated forming-free self-rectifying behaviors at an ultralow operating current (below 200 nA) by optimizing the current compliance and operating voltage. The set and reset voltages were reduced using a thin AlN layer, and the voltages' on/off ratio and rectifying ratio were as high as 80 and 102, respectively. In addition, the device showed an endurance of 103 dc cycles and a retention time over 105 s.

  14. Sustained morphologic changes to the shoreface related to Hurricane Sandy: Fire Island, NY

    NASA Astrophysics Data System (ADS)

    Hapke, C. J.; Nelson, T. R.

    2014-12-01

    A variety of topographic and photographic data have revealed widespread impacts to the subaerial portion of the beach system during Hurricane Sandy in 2012 - from beach erosion to barrier island breaching. However, less is known about impacts to the offshore environment. In order to examine a more comprehensive response of the active beach system to Hurricane Sandy, we quantify morphologic changes to the shoreface using bathymetric data along the length of Fire Island, NY. Airborne bathymetric lidar data, collected two days before Sandy made landfall, are used as a pre-storm baseline. Morphologic changes are measured using field surveyed GPS profiles collected along a limited portion of western Fire Island two months following Sandy and along the length of the island one year after Sandy. The offshore extent of the data is variable and analyses are constrained by the resolving ability of the lidar sensor, which typically achieved penetration to depths of 6-8m. The surfzone morphology was extensively impacted by Hurricane Sandy and continued to evolve during subsequent winter storms. As is typical during large storm events, the outer bar moved offshore during Sandy. A year after the storm, however, the bar remains further offshore than it was prior to Sandy, resulting in a widened surfzone. Both the subaerial and submarine portions of the beach system lost considerable volumes of sand. The majority of the loss below mean high water (MHW) is in the surfzone, concentrated in the trough immediately landward of the outer bar and generally in the pre-storm location of the outer bar. The trough not only deepened in response to Sandy, but in many locations continued to deepen over the course of the following year, with an average change of more than a meter. The average volume of the shoreface seaward of the bar increased, which is attributed to the seaward translation of the outer bar and additional offshore transport and deposition of material from the inner surfzone

  15. Source apportionment and measurement of PCBs and POPs in NY/NJ area

    NASA Astrophysics Data System (ADS)

    Du, Songyan

    used to apportion the source of PCBs in Delaware River and NY/NJ Harbor Estuary. The analysis of ambient water either generally corroborates the PCB loading estimate used in the water quality model or identified factors associated with top loading categories in previous mass balance study.

  16. Extreme metasomatism during high-P-T contact metamorphism in southeastern NY, and its tectonic implications

    NASA Astrophysics Data System (ADS)

    Dorfler, K. M.; Tracy, R. J.; Caddick, M. J.

    2011-12-01

    An important goal of high-T metamorphic petrology is to link the micrometer-scale details of mineral assemblages and textures to large-scale tectonic processes, by understanding the evolution of metamorphic conditions such as pressure and temperature. Pelitic schists from contact aureoles around mafic-ultramafic plutons in Westchester County, NY record a high-P (ca. 0.8 GPa) high-T (up to 775°C) contact overprint on a Taconic regional metamorphic assemblage (ca. 0.55 GPa). The contact metamorphic assemblage of a sample in the innermost aureole of the Croton Falls pluton, a small (<10 km2) gabbroic pluton, consists of qz+plg+bt+grt+sil+ilm+gr+Zn-rich Al-spl, with neither kfs nor mu, and with abundant bt, plg, sil, and qz inclusions within euhedral garnet crystals. Unusually low Na and K contents imply depletion of alkalic components and silica through anatexis and melt extraction during contact heating. This can be verified by tracing depleted lithologies in the contact aureole into typical metapelites outside the aureole. Theromobarometry on nearby samples lacking a contact overprint yields 620-640°C and 0.5-0.6 GPa. In the aureole sample, WDS X-ray maps show distinct Ca-enriched rims on both grt and matrix plg and significantly higher Mg content in bt inclusions within grt than in matrix bt. GASP and Grt-Bt Mg-Fe equilibria on the garnet host and inclusions interior to the high-Ca rim zone yield P=0.53 GPa and T=625°C. Pairs in the modified rim zone yield 0.8 GPa and 766°C. THERMOCALC average P-T calculations yield similar results for core (0.52 GPa, 648°C) and rim (0.78 GPa, 763°C) equilibria. These multi-phase equilibria suggest a degree of disequilibrium between phases, presumably reflecting partial resetting after metamorphic peak conditions. Garnet porphyroblasts grown during regional metamorphism at 465 Ma were substantially re-equilibrated during the contact event (~ 435 Ma). Pervasive melting at this stage is associated with development of a high

  17. A cross-taxa phenological dataset from Mohonk Lake, NY and its relationship to climate

    NASA Astrophysics Data System (ADS)

    Cook, B. I.; Cook, E. R.; Huth, P. C.; Thompson, J. E.; Forster, A.; Smiley, D.

    2007-12-01

    We present a detailed analysis of a rare cross-taxa native species phenology dataset (plant flowering, insect first sighting, and amphibian first sighting) from Mohonk Lake, NY. This dataset is highly unusual in North America for its longevity of record, consistency of methodology and location, diversity of species available, and availability of local daily meteorological data. For each phenology series, we examined the flowering and first sighting Julian calendar dates for the existence of temporal trends. Only one of the five animal species (katydid) showed any evidence for a significant trend in first sighting. In contrast, the plant species showed a rich mixture of temporal trends in flowering that could be divided into four classes: woody plant (no trend), woody plant (negative trend), herbaceous plant (negative trend), and herbaceous plant (positive trend). Many of the trends were found to be statistically significant and robust to the method of trend estimation. The data within each of the four plant classes were also pooled as anomalies to provide more complete temporal coverage for tests of trend robustness. The results were strongly consistent with the individual species trends within each class and highly significant statistically. We next correlated the flowering and first sighting dates against growing degree-day (GDD) summations for each day of the year to measure the sensitivity of each species to this common form of climatic forcing on phenology. All species showed a significant sensitivity to GDD summations, with peak correlations falling on or near the varying median flowering or first sighting dates. These results were robust whether the GDD analyses were conducted over the complete set of observations for each species (beginning on or after 1928) or over a latter period with a more serially complete set of cross-taxa observations (1970-2002). The GDD correlations indicate significant climate sensitivity in all species, but the different

  18. Expression of programmed cell death ligand 1 (PD-L1) and prevalence of tumor-infiltrating lymphocytes (TILs) in chordoma

    PubMed Central

    Feng, Yong; Shen, Jacson; Gao, Yan; Liao, Yunfei; Cote, Gregory; Choy, Edwin; Chebib, Ivan; Mankin, Henry; Hornicek, Francis; Duan, Zhenfeng

    2015-01-01

    Chordomas are primary malignant tumors of the notochord that are resistant to conventional chemotherapy. Expression of programmed cell death ligand 1 (PD-L1), prevalence of tumor-infiltrating lymphocytes (TILs), and their clinical relevance in chordoma remain unknown. We evaluated PD-L1 expression in three chordoma cell lines and nine chordoma tissue samples by western blot. Immunohistochemical staining was performed on a chordoma tissue microarray (TMA) that contained 78 tissue specimens. We also correlated the expression of PD-L1 and TILs with clinical outcomes. PD-L1 protein expression was demonstrated to be induced by IFN-γ in both UCH1 and UCH2 cell lines. Across nine human chordoma tissue samples, PD-L1 protein was differentially expressed. 94.9% of chordoma samples showed positive PD-L1 expression in the TMA. The expression score of PD-L1 for metastatic chordoma tumors was significant higher as compared with non-metastatic chordoma tumors. Expression of PD-L1 protein significantly correlates with the presence of elevated TILs, which correlates with metastasis. In summary, our study showed high levels of PD-L1 are expressed in chordoma, which is correlated with the prevalence of TILs. The current study suggests targeting PD-L1 may be a novel immunotherapeutic strategy for chordoma clinical trials. PMID:25871477

  19. Immobilization of biofilms of Pseudomonas aeruginosa NY3 and their application in the removal of hydrocarbons from highly concentrated oil-containing wastewater on the laboratory scale.

    PubMed

    Nie, Maiqian; Nie, Hongyun; He, Meili; Lin, Yingying; Wang, Lei; Jin, Pengkang; Zhang, SenYuan

    2016-05-15

    To explore the potential of Pseudomonas aeruginosa NY3 for the treatment of highly concentrated crude oil-contaminated water, the immobilization of strain NY3 on the surface of polyurethane foam (PUF), the conditions for using these biofilms and the possibility of recovering the used biofilms were studied. The results demonstrated that the biofilm formation process for strain NY3 was quick and easy. Under optimum conditions, the biomass immobilized on the PUF surface could reach 488.32 mg dry cell/g dry PUF. The results demonstrated that when the degradation time was 12 h, the average oil removal rate in 2 g crude oil/L contaminated water was approximately 90% for 40d. Meanwhile, the biofilms could be recovered for reuse. The recovery ability and the high and steady oil removal rate facilitated the application of the biofilms for the removal of concentrated oil from wastewater. PMID:26963906

  20. Comparison of Temperature and Ozone Measured by the AROTEL Instrument on DC8 Overflights of Ny Aalesund during the SOLVE Mission

    NASA Technical Reports Server (NTRS)

    Hoegy, Walter R.; McGee, Thomas J.; Burris, John F.; Heaps, William; Silbert, Donald; Sumnicht, Grant; Twigg, Laurence; Neuber, Roland

    2000-01-01

    The AROTEL instrument, deployed on the NASA DC-8 at Kiruna, Sweden for the SAGE III Ozone Loss and Validation Experiment (SOLVE), flew over the NDSC station operated by the Alfred Wegner Institute at Ny Aalesund, Spitsbergen. AROTEL ozone and temperature measurements made during near overflights of Ny Aalesund are compared with sonde ozone and temperature, and lidar ozone measurements from the NDSC station. Nine of the seventeen science flights during the December through March measurement period overflew near Ny Aalesund. Agreement of AROTEL with the ground-based temperature and ozone values at altitudes from just above the aircraft to about 30 km gives strong confidence in using AROTEL temperature and ozone mixing ratio to study the mechanisms of ozone loss in the winter arctic polar region.

  1. Surface radiation during the total solar eclipse over Ny-Ålesund, Svalbard, on 20 March 2015

    NASA Astrophysics Data System (ADS)

    Maturilli, Marion; Ritter, Christoph

    2016-04-01

    On 20 March 2015, a total solar eclipse occurred over Ny-Ålesund (78.9° N, 11.9° E), Svalbard, in the high Arctic. It was the first time that the surface radiation components during the totality of a solar eclipse were measured by a Baseline Surface Radiation Network (BSRN) station. With the Ny-Ålesund long-term radiation data set as background (available at doi:10.1594/PANGAEA.150000), we present here the peculiarities of the radiation components and basic meteorology observed during the eclipse event. The supplementary data set contains the basic BSRN radiation and surface meteorological data in 1 min resolution for March 2015, and is available at doi:10.1594/PANGAEA.854326. The eclipse radiation data will be a useful auxiliary data set for further studies on micrometeorological surface-atmosphere exchange processes in the Svalbard environment, and may serve as a test case for radiative transfer studies.

  2. MAX-DOAS measurements of atmospheric trace gases in Ny-Ålesund - Radiative transfer studies and their application

    NASA Astrophysics Data System (ADS)

    Wittrock, F.; Oetjen, H.; Richter, A.; Fietkau, S.; Medeke, T.; Rozanov, A.; Burrows, J. P.

    2004-06-01

    A new approach to derive tropospheric concentrations of some atmospheric trace gases from ground-based UV/vis measurements is described. The instrument, referred to as the MAX-DOAS, is based on the well-known UV/vis instruments, which use the sunlight scattered in the zenith sky as the light source and the method of Differential Optical Absorption Spectroscopy (DOAS) to derive column amounts of absorbers like ozone and nitrogen dioxide. Substantial enhancements have been applied to this standard setup to use different lines of sight near to the horizon as additional light sources (MAX - multi axis). Results from measurements at Ny-Ålesund (79° N, 12° E) are presented and interpreted with the full-spherical radiative transfer model SCIATRAN. In particular, measurements of the oxygen dimer O4 which has a known column and vertical distribution in the atmosphere are used to evaluate the sensitivity of the retrieval to parameters such as multiple scattering, solar azimuth, surface albedo and refraction in the atmosphere and also to validate the radiative transfer model. As a first application, measurements of NO2 emissions from a ship lying in Ny-Ålesund harbour are presented. The results of this study demonstrate the feasibility of long term UV/vis multi axis measurement that can be used to derive not only column amounts of different trace gases but also some information on the vertical location of these absorbers.

  3. THE BANANA PROJECT. III. SPIN-ORBIT ALIGNMENT IN THE LONG-PERIOD ECLIPSING BINARY NY CEPHEI

    SciTech Connect

    Albrecht, Simon; Winn, Joshua N.; Carter, Joshua A.; Snellen, Ignas A. G.; De Mooij, Ernst J. W.

    2011-01-10

    Binaries are not always neatly aligned. Previous observations of the DI Her system showed that the spin axes of both stars are highly inclined with respect to one another and the orbital axis. Here, we report on a measurement of the spin-axis orientation of the primary star of the NY Cep system, which is similar to DI Her in many respects: it features two young early-type stars ({approx}6 Myr, B0.5V+B2V), in an eccentric and relatively long-period orbit (e = 0.48, P = 15fd3). The sky projections of the rotation vector and the spin vector are well aligned ({beta}{sub p} = 2{sup 0} {+-} 4{sup 0}), in strong contrast to DI Her. Although no convincing explanation has yet been given for the misalignment of DI Her, our results show that the phenomenon is not universal, and that a successful theory will need to account for the different outcome in the case of NY Cep.

  4. Characterizing the distribution of selected PBDEs in soil, moss and reindeer dung at Ny-Ålesund of the Arctic.

    PubMed

    Wang, Zhen; Na, Guangshui; Ma, Xindong; Ge, Linke; Lin, Zhongsheng; Yao, Ziwei

    2015-10-01

    Distribution of 12 selected polybrominated diphenyl ethers (PBDEs) was characterized in soil, moss and reindeer dung samples collected simultaneously at Ny-Ålesund of the Arctic. The average PBDE concentrations were 42 pg/g (dry weight) in soil, 122 pg/g in moss and 72 pg/g in reindeer dung. Significant log/log-linear relationship was observed between the soil/moss quotients (QSM) and the sub-cooled liquid vapor pressures of PBDEs (r(2)=0.80). Moreover, excellent log/log-linear relationships between QSM and the octanol/air partition coefficients as well as between the moss/dung quotient (QMD) and the octanol/water partition coefficients of PBDEs were also observed, indicating that the physicochemical properties of PBDEs are appropriate parameters for characterizing the distribution of PBDEs in soil, moss and reindeer dung at Ny-Ålesund. Capsule abstract: Significant log-linear correlations were observed between physicochemical properties of PBDEs and their soil/moss (moss/dung) quotients. PMID:25965290

  5. Characterizing the distribution of selected PBDEs in soil, moss and reindeer dung at Ny-Ålesund of the Arctic.

    PubMed

    Wang, Zhen; Na, Guangshui; Ma, Xindong; Ge, Linke; Lin, Zhongsheng; Yao, Ziwei

    2015-10-01

    Distribution of 12 selected polybrominated diphenyl ethers (PBDEs) was characterized in soil, moss and reindeer dung samples collected simultaneously at Ny-Ålesund of the Arctic. The average PBDE concentrations were 42 pg/g (dry weight) in soil, 122 pg/g in moss and 72 pg/g in reindeer dung. Significant log/log-linear relationship was observed between the soil/moss quotients (QSM) and the sub-cooled liquid vapor pressures of PBDEs (r(2)=0.80). Moreover, excellent log/log-linear relationships between QSM and the octanol/air partition coefficients as well as between the moss/dung quotient (QMD) and the octanol/water partition coefficients of PBDEs were also observed, indicating that the physicochemical properties of PBDEs are appropriate parameters for characterizing the distribution of PBDEs in soil, moss and reindeer dung at Ny-Ålesund. Capsule abstract: Significant log-linear correlations were observed between physicochemical properties of PBDEs and their soil/moss (moss/dung) quotients.

  6. Petrology and geodynamical interpretation of mantle xenoliths from Late Cretaceous lamprophyres, Villány Mts (S Hungary)

    NASA Astrophysics Data System (ADS)

    Nédli, Zsuzsanna; M. Tóth, Tivadar; Downes, Hilary; Császár, Géza; Beard, Andrew; Szabó, Csaba

    2010-06-01

    A Late Cretaceous lamprophyre dyke in the Villány Mts (S Hungary), situated in the Tisza unit, contains abundant spinel lherzolite xenoliths with porphyroclastic textures. Mineral chemistry suggests a relatively fertile mantle, which experienced only 5-7% melt extraction. Differences in porphyroclast and neoblast chemistry and thermobarometric calculations suggest that the mantle section represented by the xenoliths experienced recrystallization at lower PT as it was transported to shallow mantle depths close to the plagioclase stability field, followed by later relaxation. Based on volcanological and sedimentological constraints from the Villány Mts and the neighboring Mecsek Mts, we suggest that the uprise of the subcontinental mantle material was related to a Cretaceous rifting event and lithospheric deformation of the southwestern part of the Tisza unit. Mantle upwelling and formation of lamprophyre melts can be related to generation or reactivation of deep fractures of the lithosphere, during a period of lithospheric extension between the major nappe emplacements (Albian-Cenomanian and Paleocene) of the region.

  7. Discovery of Polarized Emission from Two Soft X-ray-emitting Intermediate Polars: UU Col and NY Lup

    NASA Astrophysics Data System (ADS)

    Katajainen, S.; Butters, O.; Norton, A. J.; Lehto, H. J.; Piirola, V.; Berdyugin, A.

    2010-11-01

    We aim to investigate the magnetic field strengths and cyclotron emission of the two soft X-ray-emitting intermediate polars (IPs) UU Col and NY Lup. We study the connection between polars and soft X-ray-emitting IPs by searching for evidence of circularly polarized light in these two systems, which may be examples of progenitors of polars. We carried out photopolarimetric observations of our targets using the Very Large Telescope (UT2) and FORS1 at Paranal. Imaging polarimetry with good signal-to-noise and relatively high time resolution is possible for these targets using such a large telescope. Detection of circular polarization, modulated according to a white dwarf (WD) spin period, is clear evidence of cyclotron emission processes near the WD surface. The color dependence of the polarization allows us to make estimates of the magnetic field strength. We found that both UU Col and NY Lup emit circularly polarized light in the B and I bands, modulated at the spin period of the WD in each case. We add further confirmation to the idea that soft X-ray-emitting IPs emit circularly polarized light and that cyclotron emission plays an important role in these systems. This also suggests that some soft X-ray-emitting IPs might be progenitors of polars.

  8. DISCOVERY OF POLARIZED EMISSION FROM TWO SOFT X-RAY-EMITTING INTERMEDIATE POLARS: UU Col AND NY Lup

    SciTech Connect

    Katajainen, S.; Lehto, H. J.; Berdyugin, A.; Butters, O.; Norton, A. J.; Piirola, V.

    2010-11-20

    We aim to investigate the magnetic field strengths and cyclotron emission of the two soft X-ray-emitting intermediate polars (IPs) UU Col and NY Lup. We study the connection between polars and soft X-ray-emitting IPs by searching for evidence of circularly polarized light in these two systems, which may be examples of progenitors of polars. We carried out photopolarimetric observations of our targets using the Very Large Telescope (UT2) and FORS1 at Paranal. Imaging polarimetry with good signal-to-noise and relatively high time resolution is possible for these targets using such a large telescope. Detection of circular polarization, modulated according to a white dwarf (WD) spin period, is clear evidence of cyclotron emission processes near the WD surface. The color dependence of the polarization allows us to make estimates of the magnetic field strength. We found that both UU Col and NY Lup emit circularly polarized light in the B and I bands, modulated at the spin period of the WD in each case. We add further confirmation to the idea that soft X-ray-emitting IPs emit circularly polarized light and that cyclotron emission plays an important role in these systems. This also suggests that some soft X-ray-emitting IPs might be progenitors of polars.

  9. On the spin modulated circular polarization from the intermediate polars NY Lup and IGR J15094-6649

    NASA Astrophysics Data System (ADS)

    Potter, Stephen B.; Romero-Colmenero, Encarni; Kotze, Marissa; Zietsman, Ewald; Butters, O. W.; Pekeur, Nikki; Buckley, David A. H.

    2012-03-01

    We report on high-time-resolution, high-signal-to-noise ratio (S/N), photopolarimetry of the intermediate polars NY Lup and IGR J15094-6649. Our observations confirm the detection and colour dependence of circular polarization from NY Lup and additionally show a clear white dwarf, spin modulated signal. From our new high-S/N photometry, we have unambiguously detected wavelength-dependent spin and beat periods and harmonics thereof. IGR J15094-6649 is also discovered to have a particularly strong spin modulated circularly polarized signal. It appears double peaked through the I filter and single peaked through the B filter, consistent with cyclotron emission from a white dwarf with a relatively strong magnetic field. We discuss the implied accretion geometries in these two systems and any bearing this may have on the possible relationship with the connection between polars and soft X-ray-emitting intermediate polars. The relatively strong magnetic fields are also suggestive of them being polar progenitors.

  10. Deep Sequencing of T-Cell Receptor DNA as a biomarker of clonally expanded TILs in breast cancer after immunotherapy

    PubMed Central

    Page, David B.; Yuan, Jianda; Redmond, David; Wen, Y Hanna; Durack, Jeremy C.; Emerson, Ryan; Solomon, Stephen; Dong, Zhiwan; Wong, Phillip; Comstock, Christopher; Diab, Adi; Sung, Janice; Maybody, Majid; Morris, Elizabeth; Brogi, Edi; Morrow, Monica; Sacchini, Virgilio; Elemento, Olivier; Robins, Harlan; Patil, Sujata; Allison, James P.; Wolchok, Jedd D.; Hudis, Clifford; Norton, Larry; McArthur, Heather

    2016-01-01

    In early stage breast cancer, the degree of tumor-infiltrating lymphocytes (TILs) predicts response to chemotherapy and overall survival. Combination immunotherapy with immune checkpoint antibody plus tumor cryoablation can induce lymphocytic infiltrates and improve survival in mice. We used T-cell receptor (TCR) DNA sequencing to evaluate both the effect of cryo-immunotherapy in humans and the feasibility of TCR sequencing in early-stage breast cancer. In a pilot clinical trial, 18 women with early-stage breast cancer were treated preoperatively with cryoablation, single-dose anti-CTLA-4 (ipilimumab), or cryoablation + ipilimumab. TCRs within serially collected peripheral blood and tumor tissue were sequenced. In baseline tumor tissues, T-cell density as measured by TCR sequencing correlated with TIL scores obtained by hematoxylin and eosin (H&E) staining. However, tumors with little or no lymphocytes by H&E contained up to 3.6 × 106 TCR DNA sequences, highlighting the sensitivity of the ImmunoSEQ platform. In this dataset, ipilimumab increased intratumoral T-cell density over time, whereas cryoablation ± ipilimumab diversified and remodeled the intratumoral T-cell clonal repertoire. Compared to monotherapy, cryoablation plus ipilimumab was associated with numerically greater numbers of peripheral blood and intratumoral T-cell clones expanding robustly following therapy. In conclusion, TCR sequencing correlates with H&E lymphocyte scoring, and provides additional information on clonal diversity. These findings support further study of the use of TCR sequencing as a biomarker for T cell responses to therapy and for the study of cryo-immunotherapy in early-stage breast cancer. PMID:27587469

  11. Influences on the Morphologic Response to Hurricane Sandy: Fire Island, NY (Invited)

    NASA Astrophysics Data System (ADS)

    Hapke, C. J.; Brenner, O.; Schwab, W. C.

    2013-12-01

    Hurricane Sandy was the largest storm on historical record in the Atlantic basin. The highest waves and storm surge were focused along the New York and New Jersey coasts. At the height of the storm, a wave buoy 55 km offshore of Fire Island, NY recorded a significant wave height of 9.6 m. Storm-tide levels on the beach reached 4.1 m. Field surveys of the beach and dunes collected just prior to and after landfall were used to quantify morphologic change in several focus areas. Pre-storm (May 2012) and post-storm (November 2012) lidar and aerial photography are used to quantify morphologic change along the length of the island including shoreline and beach change, and volumetric change to the beach and dunes. The extent and thicknesses of washover deposits were also mapped in the field and measurements were used to determine washover volume, distribution and characteristics. The beaches and dunes on Fire Island were severely eroded, and the island breached in three locations on the eastern segment of the island. Landward retreat of the beach averaged -26 m but varied substantially along the coast. The beaches and dunes lost over 50% of their pre-storm volume, and the dunes experienced overwash along 50% of the island. Shoreline change was highly variable with an average progradational trend of 11.4 m, likely due to the deposition of material from the upper beach and dunes onto the lower portion of the beach. Although the entire island experienced extreme erosion in the form of volume loss, beach deflation and dune leveling, the central portion of Fire Island experienced the least impact. Volumetric loss of the beach and dune and overwash extent and volume were lowest in the central segment of the island. Beach and dune volume loss was similar in magnitude on the eastern and western segments of the island, but overwash deposition extent and volume were significantly greater to the east. The variation in the response of the island during Sandy parallels the

  12. Real-time Monitoring Network to Characterize Anthropogenic and Natural Events Affecting the Hudson River, NY

    NASA Astrophysics Data System (ADS)

    Islam, M. S.; Bonner, J. S.; Fuller, C.; Kirkey, W.; Ojo, T.

    2011-12-01

    The Hudson River watershed spans 34,700 km2 predominantly in New York State, including agricultural, wilderness, and urban areas. The Hudson River supports many activities including shipping, supplies water for municipal, commercial, and agricultural uses, and is an important recreational resource. As the population increases within this watershed, so does the anthropogenic impact on this natural system. To address the impacts of anthropogenic and natural activities on this ecosystem, the River and Estuary Observatory Network (REON) is being developed through a joint venture between the Beacon Institute, Clarkson University, General Electric Inc. and IBM Inc. to monitor New York's Hudson and Mohawk Rivers in real-time. REON uses four sensor platform types with multiple nodes within the network to capture environmentally relevant episodic events. Sensor platform types include: 1) fixed robotic vertical profiler (FRVP); 2) mobile robotic undulating platform (MRUP); 3) fixed acoustic Doppler current profiler (FADCP) and 4) Autonomous Underwater Vehicle (AUV). The FRVP periodically generates a vertical profile with respect to water temperature, salinity, dissolved oxygen, particle concentration and size distribution, and fluorescence. The MRUP utilizes an undulating tow-body tethered behind a research vessel to measure the same set of water parameters as the FRVP, but does so 'synchronically' over a highly-resolved spatial regime. The fixed ADCP provides continuous water current profiles. The AUV maps four-dimensional (time, latitude, longitude, depth) variation of water quality, water currents and bathymetry along a pre-determined transect route. REON data can be used to identify episodic events, both anthropogenic and natural, that impact the Hudson River. For example, a strong heat signature associated with cooling water discharge from the Indian Point nuclear power plant was detected with the MRUP. The FRVP monitoring platform at Beacon, NY, located in the

  13. Processing and cross-presentation of individual HLA-A, -B, or -C epitopes from NY-ESO-1 or an HLA-A epitope for Melan-A differ according to the mode of antigen delivery.

    PubMed

    Robson, Neil C; McAlpine, Tristan; Knights, Ashley J; Schnurr, Max; Shin, Amanda; Chen, Weisan; Maraskovsky, Eugene; Cebon, Jonathan

    2010-07-15

    The ability of dendritic cells (DCs) to cross-present protein tumor antigens to cytotoxic T lymphocytes (CTLs) underpins the success of therapeutic cancer vaccines. We studied cross-presentation of the cancer/testis antigen, NY-ESO-1, and the melanoma differentiation antigen, Melan-A by human DC subsets. Monocyte-derived DCs (MoDCs) efficiently cross-presented human leukocyte associated (HLA)-A2-restricted epitopes from either a formulated NY-ESO-1/ISCOMATRIX vaccine or when either antigen was mixed with ISCOMATRIX adjuvant. HLA-A2 epitope generation required endosomal acidification and was proteasome-independent for NY-ESO-1 and proteasome-dependent for Melan-A. Both MoDCs and CD1c(+) blood DCs cross-presented NY-ESO-1-specific HLA-A2(157-165)-, HLA-B7(60-72)-, and HLA-Cw3(92-100)-restricted epitopes when formulated as an NY-ESO-1/ISCOMATRIX vaccine, but this was limited when NY-ESO-1 and ISCOMATRIX adjuvant were added separately to the DC cultures. Finally, cross-presentation of NY-ESO-1(157-165)/HLA-A2, NY-ESO-1(60-72)/HLA-B7, and NY-ESO-1(92-100)/HLA-Cw3 epitopes was proteasome-dependent when formulated as immune complexes (ICs) but only proteasome-dependent for NY-ESO-1(60-72)/HLA-B7-restricted cross-presentation facilitated by ISCOMATRIX adjuvant. We demonstrate, for the first time, proteasome-dependent and independent cross-presentation of HLA-A-, B-, and C-restricted epitopes within the same full-length tumor antigen by human DCs. Our findings identify important differences in the capacities of human DC subsets to cross-present clinically relevant, full-length tumor antigens and how vaccine formulation impacts CTL responses in vivo.

  14. Evidence of the Adoption and Implementation of a Statewide Childhood Obesity Prevention Initiative in the New York State WIC Program: The "NY Fit WIC" Process Evaluation

    ERIC Educational Resources Information Center

    Sekhobo, Jackson P.; Egglefield, Katherine; Edmunds, Lynn S.; Shackman, Gene

    2012-01-01

    Process evaluations are critical in determining whether outcome evaluations are warranted. This study assessed the extent to which a childhood obesity prevention initiative, "NY Fit WIC", was adopted and implemented by the New York State Supplemental Nutrition Program for Women, Infants and Children (WIC). Process data came from qualitative…

  15. Optimal design of light trapping in thin-film solar cells enhanced with graded SiNx and SiOxNy structure.

    PubMed

    Zhao, Yongxiang; Chen, Fei; Shen, Qiang; Zhang, Lianmeng

    2012-05-01

    In this paper, a graded SiNx and SiOxNy structure is proposed as antireflection coatings deposited on top of amorphous silicon (α-Si) thin-film solar cell. The structural parameters are optimized by differential evolution in order to enhance the optical absorption of solar cells to the greatest degree. The optimal design result demonstrates that the nonlinear profile of dielectric constant is superior to the linear profile, and discrete multilayer graded antireflection coatings can outperform near continuously graded antireflection coatings. What's more, the electric field intensity distributions clearly demonstrate the proposed graded SiNx and SiOxNy structure can remarkably increase the magnitude of electric field of a-Si:H layer and hence, enhance the light trapping of a-Si:H thin-film solar cells in the whole visible and near-infrared spectrum. Finally, we have compared the optical absorption enhancements of proposed graded SiNx and SiOxNy structure with nanoparticles structure, and demonstrated that it can result in higher enhancements compared to the dielectric SiC and TiO2 nanoparticles. We have shown that the optimal graded SiNx and SiOxNy structure optimized by differential evolution can reach 33.31% enhancement which has exceeded the ideal limit of 32% of nanoparticles structure including plasmonic Ag nanoparticles, dielectric SiC and TiO2 nanoparticles. PMID:22565735

  16. Optimal design of light trapping in thin-film solar cells enhanced with graded SiNx and SiOxNy structure.

    PubMed

    Zhao, Yongxiang; Chen, Fei; Shen, Qiang; Zhang, Lianmeng

    2012-05-01

    In this paper, a graded SiNx and SiOxNy structure is proposed as antireflection coatings deposited on top of amorphous silicon (α-Si) thin-film solar cell. The structural parameters are optimized by differential evolution in order to enhance the optical absorption of solar cells to the greatest degree. The optimal design result demonstrates that the nonlinear profile of dielectric constant is superior to the linear profile, and discrete multilayer graded antireflection coatings can outperform near continuously graded antireflection coatings. What's more, the electric field intensity distributions clearly demonstrate the proposed graded SiNx and SiOxNy structure can remarkably increase the magnitude of electric field of a-Si:H layer and hence, enhance the light trapping of a-Si:H thin-film solar cells in the whole visible and near-infrared spectrum. Finally, we have compared the optical absorption enhancements of proposed graded SiNx and SiOxNy structure with nanoparticles structure, and demonstrated that it can result in higher enhancements compared to the dielectric SiC and TiO2 nanoparticles. We have shown that the optimal graded SiNx and SiOxNy structure optimized by differential evolution can reach 33.31% enhancement which has exceeded the ideal limit of 32% of nanoparticles structure including plasmonic Ag nanoparticles, dielectric SiC and TiO2 nanoparticles.

  17. LV305, a dendritic cell-targeting integration-deficient ZVexTM-based lentiviral vector encoding NY-ESO-1, induces potent anti-tumor immune response

    PubMed Central

    Albershardt, Tina Chang; Campbell, David James; Parsons, Andrea Jean; Slough, Megan Merrill; ter Meulen, Jan; Berglund, Peter

    2016-01-01

    We have engineered an integration-deficient lentiviral vector, LV305, to deliver the tumor antigen NY-ESO-1 to human dendritic cells in vivo through pseudotyping with a modified Sindbis virus envelop protein. Mice immunized once with LV305 developed strong, dose-dependent, multifunctional, and cytotoxic NY-ESO-1-specific cluster of differentiation 8 (CD8) T cells within 14 days post-immunization and could be boosted with LV305 at least twice to recall peak-level CD8 T-cell responses. Immunization with LV305 protected mice against tumor growth in an NY-ESO-1-expressing CT26 lung metastasis model, with the protective effect abrogated upon depletion of CD8 T cells. Adoptive transfer of CD8 T cells, alone or together with CD4 T cells or natural killer cells, from LV305-immunized donor mice to tumor-bearing recipient mice conferred significant protection against metastatic tumor growth. Biodistribution of injected LV305 in mice was limited to the site of injection and the draining lymph node, and injected LV305 exhibited minimal excretion. Mice injected with LV305 developed little to no adverse effects, as evaluated by toxicology studies adherent to good laboratory practices. Taken together, these data support the development of LV305 as a clinical candidate for treatment against tumors expressing NY-ESO-1. PMID:27626061

  18. LV305, a dendritic cell-targeting integration-deficient ZVex(TM)-based lentiviral vector encoding NY-ESO-1, induces potent anti-tumor immune response.

    PubMed

    Albershardt, Tina Chang; Campbell, David James; Parsons, Andrea Jean; Slough, Megan Merrill; Ter Meulen, Jan; Berglund, Peter

    2016-01-01

    We have engineered an integration-deficient lentiviral vector, LV305, to deliver the tumor antigen NY-ESO-1 to human dendritic cells in vivo through pseudotyping with a modified Sindbis virus envelop protein. Mice immunized once with LV305 developed strong, dose-dependent, multifunctional, and cytotoxic NY-ESO-1-specific cluster of differentiation 8 (CD8) T cells within 14 days post-immunization and could be boosted with LV305 at least twice to recall peak-level CD8 T-cell responses. Immunization with LV305 protected mice against tumor growth in an NY-ESO-1-expressing CT26 lung metastasis model, with the protective effect abrogated upon depletion of CD8 T cells. Adoptive transfer of CD8 T cells, alone or together with CD4 T cells or natural killer cells, from LV305-immunized donor mice to tumor-bearing recipient mice conferred significant protection against metastatic tumor growth. Biodistribution of injected LV305 in mice was limited to the site of injection and the draining lymph node, and injected LV305 exhibited minimal excretion. Mice injected with LV305 developed little to no adverse effects, as evaluated by toxicology studies adherent to good laboratory practices. Taken together, these data support the development of LV305 as a clinical candidate for treatment against tumors expressing NY-ESO-1. PMID:27626061

  19. 75 FR 27332 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC; Eagle Creek Water Resources, LLC; Eagle Creek Land...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-14

    ... Energy Regulatory Commission AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC; Eagle Creek Water Resources... Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources, LLC.... For the transferee: Mr. Paul Ho, Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC,...

  20. 77 FR 13592 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, Eagle Creek Land...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-07

    ... Energy Regulatory Commission AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources... Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources, LLC (transferees) filed an...) 805-1469. Transferees: Mr. Bernard H. Cherry, Eagle Creek Hydro Power, LLC, Eagle Creek...

  1. LV305, a dendritic cell-targeting integration-deficient ZVex(TM)-based lentiviral vector encoding NY-ESO-1, induces potent anti-tumor immune response.

    PubMed

    Albershardt, Tina Chang; Campbell, David James; Parsons, Andrea Jean; Slough, Megan Merrill; Ter Meulen, Jan; Berglund, Peter

    2016-01-01

    We have engineered an integration-deficient lentiviral vector, LV305, to deliver the tumor antigen NY-ESO-1 to human dendritic cells in vivo through pseudotyping with a modified Sindbis virus envelop protein. Mice immunized once with LV305 developed strong, dose-dependent, multifunctional, and cytotoxic NY-ESO-1-specific cluster of differentiation 8 (CD8) T cells within 14 days post-immunization and could be boosted with LV305 at least twice to recall peak-level CD8 T-cell responses. Immunization with LV305 protected mice against tumor growth in an NY-ESO-1-expressing CT26 lung metastasis model, with the protective effect abrogated upon depletion of CD8 T cells. Adoptive transfer of CD8 T cells, alone or together with CD4 T cells or natural killer cells, from LV305-immunized donor mice to tumor-bearing recipient mice conferred significant protection against metastatic tumor growth. Biodistribution of injected LV305 in mice was limited to the site of injection and the draining lymph node, and injected LV305 exhibited minimal excretion. Mice injected with LV305 developed little to no adverse effects, as evaluated by toxicology studies adherent to good laboratory practices. Taken together, these data support the development of LV305 as a clinical candidate for treatment against tumors expressing NY-ESO-1.

  2. NY-ESO-1 is a sensitive and specific immunohistochemical marker for myxoid and round cell liposarcomas among related mesenchymal myxoid neoplasms.

    PubMed

    Hemminger, Jessica A; Iwenofu, O Hans

    2013-09-01

    Myxoid and round cell liposarcomas constitute approximately one-third of all liposarcomas, a relatively common group of fat-derived soft tissue sarcomas. The histomorphology is a continuum between highly differentiated myxoid and poorly differentiated round cell components. The gold standard of diagnosis is dependent on histomorphology and/or identification of t(12;16)(q13;p11) translocation by cytogenetics or demonstration of DDIT3 rearrangements by fluorescence in situ hybridization. There are currently no diagnostic immunohistochemical stains available. The broad range of myxoid neoplasms in the differential diagnosis includes a variety of sarcomas. Given the notable differences in disease biology among myxoid neoplasms, which range from benign to aggressive, an accurate diagnosis is imperative for proper treatment and prognostication. Prompted by our recent study showing frequent expression of the cancer testis antigen NY-ESO-1 in myxoid and round cell liposarcomas, we sought to evaluate the utility of NY-ESO-1 as an immunohistochemical marker for myxoid and round cell liposarcoma among mesenchymal myxoid neoplasms within the differential diagnosis. Formalin-fixed, paraffin-embedded blocks were obtained for the following mesenchymal myxoid neoplasms (n=138): myxoid and round cell liposarcoma (n=38); well-differentiated liposarcoma (n=12); lipoma (n=20; 4 with myxoid change); extra-cardiac soft tissue myxoma (n=39); extraskeletal myxoid chondrosarcoma (n=12); myxofibrosarcoma (n=10: 5 low grade, 2 intermediate grade, 3 high grade); and low-grade fibromyxoid sarcoma (n=7). Utilizing standard immunohistochemistry protocols, full sections were stained with NY-ESO-1 (clone E978), and staining was assessed for intensity (1-2+), percentage of tumor positivity, and location. In all, 36/38 (95%) of the myxoid and round cell liposarcomas demonstrated NY-ESO-1 immunoreactivity. The majority of the positive cases (34/36; 94%) showed strong, homogenous staining (>50% tumor

  3. Processes and sources controlling total gaseous mercury and uraban air quality in Syracuse, NY and Nanjing, China

    NASA Astrophysics Data System (ADS)

    Hall, Casey B.

    Investigations of air quality were performed in Nanjing, China during 2011 and Syracuse, NY during 2013. The regional background of total gaseous mercury (TGM) in the Yangtze River Delta (YRD) was estimated at 2.2 ng m-3. Global heterogeneity of free tropospheric TGM was hypothesized from background episodes. Emissions of TGM were underestimated up to 80% in the region. Additionally, high levels of TGM were attributed to local sources, transport, and the monsoon. An air quality station measuring CO, CO2, TGM, and O 3 was built on the campus of SUNY ESF. The one hour peak mixing ratios of CO were estimated to have declined by 59% over 2000- 2013, more than EPA estimated 53%. Regional transport of O3 increased peak mixing ratios. TGM was influenced by local sources. Finally, Lake Ontario is hypothesized to facilitate transport of trace gas species based on the diel cycles of TGM, CO, and H2O.

  4. Personal exposures to traffic-related particle pollution among children with asthma in the South Bronx, NY.

    PubMed

    Spira-Cohen, Ariel; Chen, Lung Chi; Kendall, Michaela; Sheesley, Rebecca; Thurston, George D

    2010-07-01

    Personal exposures to fine particulate matter air pollution (PM(2.5)), and to its traffic-related fraction, were investigated in a group of urban children with asthma. The relationships of personal and outdoor school-site measurements of PM(2.5) and elemental carbon (EC) were characterized for a total of 40 fifth-grade children. These students, from four South Bronx, NY schools, each carried air pollution monitoring equipment with them for 24 h per day for approximately 1 month. Daily EC concentrations were estimated using locally calibrated reflectance of the PM(2.5) samples. Personal EC concentration was more closely related to outdoor school-site EC (median subject-specific: r=0.64) than was personal PM(2.5) to school-site PM(2.5) concentration (median subject-specific: r=0.33). Regression models also showed a stronger, more robust association of school site with personal measurements for EC than those for PM(2.5). High traffic pollution exposure was found to coincide with the weekday early morning rush hour, with higher personal exposures for participants living closer to a highway (<500 ft). A significant linear relationship of home distance from a highway with personal EC pollution exposure was also found (up to 1000 ft). This supports the assumptions by previous epidemiological studies using distance from a highway as an index of traffic PM exposure. These results are also consistent with the assumption that traffic, and especially smoke emitted from diesel vehicles, is a significant contributor to personal PM exposure levels in children living in urban areas such as the South Bronx, NY.

  5. Personal exposures to traffic-related particle pollution among children with asthma in the South Bronx, NY

    PubMed Central

    Spira-Cohen, Ariel; Chen, Lung Chi; Kendall, Michaela; Sheesley, Rebecca; Thurston, George D.

    2014-01-01

    Personal exposures to fine Particulate Matter air pollution (PM2.5), and to its traffic-related fraction, were investigated in a group of urban children with asthma. The relationships of personal and outdoor school-site measurements of PM2.5 and elemental carbon (EC) were characterized for a total of forty fifth-grade children. These students, from four South Bronx, NY schools, each carried air pollution monitoring equipment with them 24 hours per day for approximately one month. Daily EC concentrations were estimated using locally calibrated reflectance of the PM2.5 samples. Personal EC was more closely related to outdoor school-site EC (median subject-specific r = 0.64) than was personal PM with school-site PM2.5 (median subject specific r = 0.33). Regression models also showed a stronger, more robust association of school-site with personal measurements for EC than for PM2.5. High traffic pollution exposure was found to coincide with the weekday early morning rush hour, with higher personal exposures for subjects living closer to a highway (< 500 ft). A significant linear relationship of home distance from a highway with personal EC pollution exposure was also found (up to 1000 ft.). This supports the assumptions by previous epidemiological studies using distance from a highway as an index of traffic PM exposure. These results are also consistent with the assumption that traffic, and especially diesel vehicles, are a significant contributor to personal PM exposure levels of children living in urban areas such as the South Bronx, NY. PMID:19865073

  6. Bi-specific TCR-anti CD3 redirected T-cell targeting of NY-ESO-1- and LAGE-1-positive tumors.

    PubMed

    McCormack, Emmet; Adams, Katherine J; Hassan, Namir J; Kotian, Akhil; Lissin, Nikolai M; Sami, Malkit; Mujić, Maja; Osdal, Tereza; Gjertsen, Bjørn Tore; Baker, Deborah; Powlesland, Alex S; Aleksic, Milos; Vuidepot, Annelise; Morteau, Olivier; Sutton, Deborah H; June, Carl H; Kalos, Michael; Ashfield, Rebecca; Jakobsen, Bent K

    2013-04-01

    NY-ESO-1 and LAGE-1 are cancer testis antigens with an ideal profile for tumor immunotherapy, combining up-regulation in many cancer types with highly restricted expression in normal tissues and sharing a common HLA-A*0201 epitope, 157-165. Here, we present data to describe the specificity and anti-tumor activity of a bifunctional ImmTAC, comprising a soluble, high-affinity T-cell receptor (TCR) specific for NY-ESO-1157-165 fused to an anti-CD3 scFv. This reagent, ImmTAC-NYE, is shown to kill HLA-A2, antigen-positive tumor cell lines, and freshly isolated HLA-A2- and LAGE-1-positive NSCLC cells. Employing time-domain optical imaging, we demonstrate in vivo targeting of fluorescently labelled high-affinity NYESO-specific TCRs to HLA-A2-, NY-ESO-1157-165-positive tumors in xenografted mice. In vivo ImmTAC-NYE efficacy was tested in a tumor model in which human lymphocytes were stably co-engrafted into NSG mice harboring tumor xenografts; efficacy was observed in both tumor prevention and established tumor models using a GFP fluorescence readout. Quantitative RT-PCR was used to analyze the expression of both NY-ESO-1 and LAGE-1 antigens in 15 normal tissues, 5 cancer cell lines, 10 NSCLC, and 10 ovarian cancer samples. Overall, LAGE-1 RNA was expressed at a greater frequency and at higher levels than NY-ESO-1 in the tumor samples. These data support the clinical utility of ImmTAC-NYE as an immunotherapeutic agent for a variety of cancers.

  7. New anatase-type Til-2xNbxAlxO2 solid solution nanoparticles: direct formation, phase stability, and photocatalytic performance.

    PubMed

    Hirano, Masanori; Ito, Takaharu

    2006-12-01

    New anatase-type titania solid solutions co-doped with niobium and aluminum (Til-2xNbxAIlxO2 (X = 0 -0.20)) were synthesized as nanoparticles from precursor solutions of TiOSO4, NbCl5, and Al(NO3)3 under mild hydrothermal conditions at 180 degrees C for 5 h using the hydrolysis of urea. The lattice parameters a0 and c0 of anatase slightly and gradually increased, when the content of niobium and aluminum increased from X = 0 to 0.20. The crystallite size of anatase increased from 12 to 28 nm with increasing the value of X from 0 to 0.20. Their photocatalytic activity and adsorptivity were evaluated separately by the measurement of the concentration of methylene blue (MB) remained in the solution in the dark or under UV-light irradiation. The adsorptivity of TiO2 was improved by the formation of anatase-type Til-2xNbxAlxO2 solid solutions. The photocatalytic activity of anatase-type Til-2xNbxAlxO2 solid solutions was superior to that of commercially available anatase-type pure TiO2 (ST-01) and anatase-type pure TiO2 hydrothermally prepared. The new anatase phase of Til-2xNbxAlxO2 (X = 0-0.20) solid solutions existed stably up to 850 0C during heat treatment in air. In comparison with hydrothermal pure TiO2, the starting temperature of anatase-to-rutile phase transformation was delayed by the formation of Ti1-2xNbxAlxO, (X = 0-0.20) solid solutions, although its completing temperature was accelerated. PMID:17256336

  8. Seminal plasma induces inflammation in the uterus through the γδ T/IL-17 pathway

    PubMed Central

    Song, Zhi-Hui; Li, Zhong-Yin; Li, Dan-Dan; Fang, Wen-Ning; Liu, Hai-Yan; Yang, Dan-Dan; Meng, Chao-Yang; Yang, Ying; Peng, Jing-Pian

    2016-01-01

    After insemination, a large number of leukocytes migrate into the uterus, which is accompanied by intense inflammation. However, the details of how seminal plasma interacts with the uterus are still not very clear. Here, we present that neutrophils migrate and accumulate around the uterine epithelium following insemination, which is accompanied by an increase in interleukin (IL) 17A levels. Additionally, we find that γδ T cells are the major source of IL-17A, and the seminal plasma could induce the γδ T cells to secret IL-17A. Blocking IL-17A could reduce the number of neutrophils in the uterus and prevent them from migrating to the epithelium by decreasing the chemokines CXCL1, CXCL2 and CXCL5. Blocking IL-17A did not affect the Th1/Th2 balance but actually diminished the inflammation in the uterus by reducing the expression of IL-1β and TNF-α. In summary, we found a new mechanism by which seminal plasma could influence the inflammation in the uterus through the γδ T/IL-17 pathway to regulate the expression of various chemokines and cytokines. PMID:27109934

  9. Methane and nitrous oxide fluxes from four tundra ecotopes in Ny-Ålesund of the High Arctic.

    PubMed

    Chen, Qingqing; Zhu, Renbin; Wang, Qing; Xu, Hua

    2014-07-01

    During the summers of 2008 and 2009, net methane (CH₄) and nitrous oxide (N₂O) fluxes were investigated from 4 tundra ecotopes: normal lowland tundra (LT), bird sanctuary tundra (BT), the tundra in an abandoned coal mine (CT) and the tundra in scientific bases (ST) in Ny-Ålesund of the High Arctic. Tundra soils in CT (184.5 ± 40.0 μg CH4/(m²·hr)) and ST (367.6 ± 92.3μg CH4/(m²·hr)) showed high CH4 emissions due to the effects of human activities, whereas high CH4 uptake or low emission occurred in the soils of LT and BT. The lowland tundra soils (mean, -4.4-4.3μg N₂O/(m²·hr)) were weak N₂O sources and even sinks. Bird activity increased N₂O emissions from BT with the mean flux of 7.9μgN2O/(m(2)·hr). The mean N₂O fluxes from CT (45.4 ± 10.2 μg N₂O/(m²·hr)) and ST (78.8 ± 18.5μg N₂O/(m²·hr)) were one order of magnitude higher than those from LT and BT, indicating that human activities significantly increased N₂O emissions from tundra soils. Soil total carbon and water regime were important factors affecting CH₄ fluxes from tundra soils. The N₂O fluxes showed a significant positive correlation with ammonia nitrogen (NH₄(+)-N) contents (r=0.66, p<0.001) at all the observation sites, indicating that ammonia nitrogen (NH₄(+)-N) content acted as a strong predictor for N₂O emissions from tundra soils. The CH4 and N₂O fluxes did not correspond to the temperature variations of soil at 0-15 cm depths. Overall our results implied that human activities might have greater effects on soil CH₄ and N₂O emissions than current climate warming in Ny-Ålesund, High Arctic.

  10. Methane and nitrous oxide fluxes from four tundra ecotopes in Ny-Ålesund of the High Arctic.

    PubMed

    Chen, Qingqing; Zhu, Renbin; Wang, Qing; Xu, Hua

    2014-07-01

    During the summers of 2008 and 2009, net methane (CH₄) and nitrous oxide (N₂O) fluxes were investigated from 4 tundra ecotopes: normal lowland tundra (LT), bird sanctuary tundra (BT), the tundra in an abandoned coal mine (CT) and the tundra in scientific bases (ST) in Ny-Ålesund of the High Arctic. Tundra soils in CT (184.5 ± 40.0 μg CH4/(m²·hr)) and ST (367.6 ± 92.3μg CH4/(m²·hr)) showed high CH4 emissions due to the effects of human activities, whereas high CH4 uptake or low emission occurred in the soils of LT and BT. The lowland tundra soils (mean, -4.4-4.3μg N₂O/(m²·hr)) were weak N₂O sources and even sinks. Bird activity increased N₂O emissions from BT with the mean flux of 7.9μgN2O/(m(2)·hr). The mean N₂O fluxes from CT (45.4 ± 10.2 μg N₂O/(m²·hr)) and ST (78.8 ± 18.5μg N₂O/(m²·hr)) were one order of magnitude higher than those from LT and BT, indicating that human activities significantly increased N₂O emissions from tundra soils. Soil total carbon and water regime were important factors affecting CH₄ fluxes from tundra soils. The N₂O fluxes showed a significant positive correlation with ammonia nitrogen (NH₄(+)-N) contents (r=0.66, p<0.001) at all the observation sites, indicating that ammonia nitrogen (NH₄(+)-N) content acted as a strong predictor for N₂O emissions from tundra soils. The CH4 and N₂O fluxes did not correspond to the temperature variations of soil at 0-15 cm depths. Overall our results implied that human activities might have greater effects on soil CH₄ and N₂O emissions than current climate warming in Ny-Ålesund, High Arctic. PMID:25079988

  11. Electrical evaluation of crack generation in SiNx and SiOxNy thin-film encapsulation layers for OLED displays

    NASA Astrophysics Data System (ADS)

    Park, Eun Kil; Kim, Sungmin; Heo, Jaeyeong; Kim, Hyeong Joon

    2016-05-01

    By measuring leakage current density, we detected crack generation in silicon nitride (SiNx) and silicon oxynitride (SiOxNy) thin-film encapsulation layers, and correlated with the films' water vapor permeability characteristics. After repeated bending cycles, both the changes in water vapor transmission rate and leakage current density were directly proportional to the crack density. Thick SiNx films had better water vapor barrier characteristics in their pristine state, but cyclic loading led to fast failure. Varying the atomic concentration of the SiOxNy films affected their bending reliability. We attribute these differences to changes in the shape of the crack tip as the oxygen content varies.

  12. Effects of an intense, high-frequency laser field on bound states in Ga1 - xInxNyAs1 - y/GaAs double quantum well.

    PubMed

    Ungan, Fatih; Yesilgul, Unal; Sakiroğlu, Serpil; Kasapoglu, Esin; Erol, Ayse; Arikan, Mehmet Cetin; Sarı, Huseyin; Sökmen, Ismail

    2012-10-31

    Within the envelope function approach and the effective-mass approximation, we have investigated theoretically the effect of an intense, high-frequency laser field on the bound states in a GaxIn1 - xNyAs1 - y/GaAs double quantum well for different nitrogen and indium mole concentrations. The laser-dressed potential, bound states, and squared wave functions related to these bound states in Ga1 - xInxNyAs1 - y/GaAs double quantum well are investigated as a function of the position and laser-dressing parameter. Our numerical results show that both intense laser field and nitrogen (indium) incorporation into the GaInNAs have strong influences on carrier localization.

  13. Oxide Charge Engineering of Atomic Layer Deposited AlOxNy/Al2O3 Gate Dielectrics: A Path to Enhancement Mode GaN Devices.

    PubMed

    Negara, M A; Kitano, M; Long, R D; McIntyre, P C

    2016-08-17

    Nitrogen incorporation to produce negative fixed charge in Al2O3 gate insulator layers is investigated as a path to achieve enhancement mode GaN device operation. A uniform distribution of nitrogen across the resulting AlOxNy films is obtained using N2 plasma enhanced atomic layer deposition (ALD). The flat band voltage (Vfb) increases to a significantly more positive value with increasing nitrogen concentration. Insertion of a 2 nm thick Al2O3 interlayer greatly decreases the trap density of the insulator/GaN interface, and reduces the voltage hysteresis and frequency dispersion of gate capacitance compared to single-layer AlOxNy gate insulators in GaN MOSCAPs.

  14. Oxide Charge Engineering of Atomic Layer Deposited AlOxNy/Al2O3 Gate Dielectrics: A Path to Enhancement Mode GaN Devices.

    PubMed

    Negara, M A; Kitano, M; Long, R D; McIntyre, P C

    2016-08-17

    Nitrogen incorporation to produce negative fixed charge in Al2O3 gate insulator layers is investigated as a path to achieve enhancement mode GaN device operation. A uniform distribution of nitrogen across the resulting AlOxNy films is obtained using N2 plasma enhanced atomic layer deposition (ALD). The flat band voltage (Vfb) increases to a significantly more positive value with increasing nitrogen concentration. Insertion of a 2 nm thick Al2O3 interlayer greatly decreases the trap density of the insulator/GaN interface, and reduces the voltage hysteresis and frequency dispersion of gate capacitance compared to single-layer AlOxNy gate insulators in GaN MOSCAPs. PMID:27459343

  15. Antibody-targeted NY-ESO-1 to mannose receptor or DEC-205 in vitro elicits dual human CD8+ and CD4+ T cell responses with broad antigen specificity.

    PubMed

    Tsuji, Takemasa; Matsuzaki, Junko; Kelly, Marcus P; Ramakrishna, Venky; Vitale, Laura; He, Li-Zhen; Keler, Tibor; Odunsi, Kunle; Old, Lloyd J; Ritter, Gerd; Gnjatic, Sacha

    2011-01-15

    Immunization of cancer patients with vaccines containing full-length tumor Ags aims to elicit specific Abs and both CD4(+) and CD8(+) T cells. Vaccination with protein Ags, however, often elicits only CD4(+) T cell responses without inducing Ag-specific CD8(+) T cells, as exogenous protein is primarily presented to CD4(+) T cells. Recent data revealed that Ab-mediated targeting of protein Ags to cell surface receptors on dendritic cells could enhance the induction of both CD4(+) and CD8(+) T cells. We investigated in this study if these observations were applicable to NY-ESO-1, a cancer-testis Ag widely used in clinical cancer vaccine trials. We generated two novel targeting proteins consisting of the full-length NY-ESO-1 fused to the C terminus of two human mAbs against the human mannose receptor and DEC-205, both internalizing molecules expressed on APC. These targeting proteins were evaluated for their ability to activate NY-ESO-1-specific human CD4(+) and CD8(+) T cells in vitro. Both targeted NY-ESO-1 proteins rapidly bound to their respective targets on APC. Whereas nontargeted and Ab-targeted NY-ESO-1 proteins similarly activated CD4(+) T cells, cross-presentation to CD8(+) T cells was only efficiently induced by targeted NY-ESO-1. In addition, both mannose receptor and DEC-205 targeting elicited specific CD4(+) and CD8(+) T cells from PBLs of cancer patients. Receptor-specific delivery of NY-ESO-1 to APC appears to be a promising vaccination strategy to efficiently generate integrated and broad Ag-specific immune responses against NY-ESO-1 in cancer patients.

  16. Nonclassical antigen-processing pathways are required for MHC class II-restricted direct tumor recognition by NY-ESO-1-specific CD4(+) T cells.

    PubMed

    Matsuzaki, Junko; Tsuji, Takemasa; Luescher, Immanuel; Old, Lloyd J; Shrikant, Protul; Gnjatic, Sacha; Odunsi, Kunle

    2014-04-01

    Tumor antigen-specific CD4(+) T cells that directly recognize cancer cells are important for orchestrating antitumor immune responses at the local tumor sites. However, the mechanisms of direct MHC class II (MHC-II) presentation of intracellular tumor antigen by cancer cells are poorly understood. We found that two functionally distinct subsets of CD4(+) T cells were expanded after HLA-DPB1*04 (DP04)-binding NY-ESO-1157-170 peptide vaccination in patients with ovarian cancer. Although both subsets recognized exogenous NY-ESO-1 protein pulsed on DP04(+) target cells, only one type recognized target cells with intracellular expression of NY-ESO-1. The tumor-recognizing CD4(+) T cells more efficiently recognized the short 8-9-mer peptides than the non-tumor-recognizing CD4(+) T cells. In addition to endosomal/lysosomal proteases that are typically involved in MHC-II antigen presentation, several pathways in the MHC class I presentation pathways, such as the proteasomal degradation and transporter-associated with antigen-processing-mediated peptide transport, were also involved in the presentation of intracellular NY-ESO-1 on MHC-II. The presentation was inhibited significantly by primaquine, a small molecule that inhibits endosomal recycling, consistent with findings that pharmacologic inhibition of new protein synthesis enhances antigen presentation. Together, our data demonstrate that cancer cells selectively present peptides from intracellular tumor antigens on MHC-II by multiple nonclassical antigen-processing pathways. Harnessing the direct tumor-recognizing ability of CD4(+) T cells could be a promising strategy to enhance antitumor immune responses in the immunosuppressive tumor microenvironment.

  17. Local structures and interface morphology of InxGa1-xAs1-yNy thin films grown on GaAs

    NASA Astrophysics Data System (ADS)

    Soo, Y. L.; Huang, S.; Kao, Y. H.; Chen, J. G.; Hulbert, S. L.; Geisz, J. F.; Kurtz, Sarah; Olson, J. M.; Kurtz, Steven R.; Jones, E. D.; Allerman, A. A.

    1999-11-01

    X-ray absorption fine-structure techniques have been utilized to probe the short-range structures around N and In in InxGa1-xAs1-yNy compounds containing about 3% of N and 8% of In. Our results indicate that N impurities most likely substitute for As atoms in the system. The In-As interatomic distance in these compounds remains practically the same as in InAs, while the coordination number of As atoms around In shows possible variations with changes in the material characteristics. The N atoms play an important role in affecting the changes of band gap while also serving as ``strain moderators'' by providing a tensile strain in the film to counteract the compressive strain caused by the In impurities. Further, grazing incidence x-ray scattering measurements of InxGa1-xAs1-yNy/GaAs heterojunctions provide direct evidence that the InxGa1-xAs1-yNy thin films can indeed be lattice matched to GaAs substrates resulting in a reasonably smooth heterointerface.

  18. Chemical synthesis of a polypeptide backbone derived from the primary sequence of the cancer protein NY-ESO-1 enabled by kinetically controlled ligation and pseudoprolines.

    PubMed

    Harris, Paul W R; Brimble, Margaret A

    2015-03-01

    The cancer protein NY-ESO-1 has been shown to be one of the most promising vaccine candidates although little is known about its cellular function. Using a chemical protein strategy, the 180 amino acid polypeptide, tagged with an arginine solubilizing tail, was assembled in a convergent manner from four unprotected peptide α-thioester peptide building blocks and one cysteinyl polypeptide, which were in turn prepared by Boc and Fmoc solid phase peptide synthesis (SPPS) respectively. To facilitate the assembly by ligation chemistries, non-native cysteines were introduced as chemical handles into the polypeptide fragments; pseudoproline dipeptides and microwave assisted Fmoc SPPS were crucial techniques to prepare the challenging hydrophobic C-terminal fragment. Three sequential kinetically controlled ligations, which exploited the reactivity between peptide arylthioesters and peptide alkylthioesters, were then used in order to assemble the more tractable N-terminal region of NY-ESO-1. The ensuing 147 residue polypeptide thioester then underwent successful final native chemical ligation with the very hydrophobic C-terminal polypeptide bearing an N-terminal cysteine affording the 186 residue polypeptide as an advanced intermediate en route to the native NY-ESO-1 protein.

  19. SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade.

    PubMed

    Xue, Wei; Metheringham, Rachael L; Brentville, Victoria A; Gunn, Barbara; Symonds, Peter; Yagita, Hideo; Ramage, Judith M; Durrant, Lindy G

    2016-06-01

    Checkpoint blockade has demonstrated promising antitumor responses in approximately 10-40% of patients. However, the majority of patients do not make a productive immune response to their tumors and do not respond to checkpoint blockade. These patients may benefit from an effective vaccine that stimulates high-avidity T cell responses in combination with checkpoint blockade. We have previously shown that incorporating TRP-2 and gp100 epitopes into the CDR regions of a human IgG1 DNA (ImmunoBody®: IB) results in significant tumor regression both in animal models and patients. This vaccination strategy is superior to others as it targets antigen to antigen-presenting cells and stimulates high-avidity T cell responses. To broaden the application of this vaccination strategy, 16 NY-ESO-1 epitopes, covering over 80% of HLA phenotypes, were incorporated into the IB (SCIB2). They produced higher frequency and avidity T cell responses than peptide vaccination. These T cells were of sufficient avidity to kill NY-ESO-1-expressing tumor cells, and in vivo controlled the growth of established B16-NY-ESO-1 tumors, resulting in long-term survival (35%). When SCIB2 was given in combination with Treg depletion, CTLA-4 blockade or PD-1 blockade, long-term survival from established tumors was significantly enhanced to 56, 67 and 100%, respectively. Translating these responses into the clinic by using a combination of SCIB2 vaccination and checkpoint blockade can only further improve clinical responses.

  20. Production of a novel bioflocculant MNXY1 by Klebsiella pneumoniae strain NY1 and application in precipitation of cyanobacteria and municipal wastewater treatment

    PubMed Central

    Nie, M.; Yin, X.; Jia, J.; Wang, Y.; Liu, S.; Shen, Q.; Li, P.; Wang, Z.

    2015-01-01

    Aims To isolate and characterize the novel bioflocculant-producing bacteria, to optimize the bioflocculant production and evaluate its potential applications. Methods and Results Klebsiella pneumoniae strain NY1, a bacterium that produces a novel bioflocculant (MNXY1), was selected on the chemically defined media. It was classified according to the 16S rRNA gene sequence, morphological and microscopic characteristics. MNXY1 was characterized to contain 26% protein and 66% total sugar. The constituent sugar monomers of MNXY1, revealed by NMR analysis, are glucose, galactose and quinovose. Favorable culture conditions for MNXY1 production were determined. Strain NY1 produces a high level (14.9 g l−1) of MNXY1. MNXY1 is thermostable and tolerant to the extreme pH. It precipitated 54% of cyanobacteria from laboratory culture and 72% of the total suspended solids from raw wastewater. Conclusions Strain NY1 was identified to produce a novel bioflocculant MNXY1. The outstanding performance of MNXY1 in practical applications and its availability in copious amounts make it attractive for further investigation and development for industrial scale applications. PMID:21679283

  1. Diversity and distribution of cultured endolichenic fungi in the Ny-Ålesund Region, Svalbard (High Arctic).

    PubMed

    Zhang, Tao; Wei, Xin-Li; Wei, Yu-Zhen; Liu, Hong-Yu; Yu, Li-Yan

    2016-07-01

    Endolichenic fungi within 17 lichen species in the area near Ny-Ålesund (Svalbard, High Arctic) were studied by a culture-based method. The 247 fungal isolates were obtained from 2712 lichen thallus segments. The colonization rate of endolichenic fungi ranged from 1.6 to 26.5 %, respectively. These isolates were identified to 40 fungal taxa, including 35 Ascomycota (10 orders), 4 Basidiomycota (3 orders), and 1 unidentified fungus. Thelebolales was the most abundant order, while Sordariales were the most diverse order. The common fungal taxa shared by more than 3 lichen species were Thelebolus microsporus (93 isolates), Coniochaeta hoffmannii (7 isolates), Sarocladium kiliense (33 isolates), Coniochaeta sp. 1 (5 isolates), Coniochaeta sp. 4 (28 isolates), and Coniochaeta sp. 2 (5 isolates). Low Sorenson's similarity coefficients were observed among different lichen species, indicating that host-related factor may shape the endolichenic fungal communities in this region. In addition, no endolichenic fungal taxa were previously found in the Antarctica and Austrian Alps, suggesting endolichenic fungal communities in this region might be also shaped by the Arctic climate. The results demonstrate the existence of specific cultured endolichenic fungal species, which may be suitable objects for further study of their possible functional roles in the lichen thalli. PMID:27240672

  2. Water Residence Times and Their Relation to Soil and Aquifer Properties and Degree of Urbanization (Croton Water Supply Area, NY)

    NASA Astrophysics Data System (ADS)

    Vitvar, T.; Burns, D.; Kendall, C.; McDonnell, J.

    2002-05-01

    Water residence times were determined in 3 small watersheds in the Croton water supply area, NY. The watersheds (less than 1 km2 drainage area) have different amounts of urbanization (natural, semi-developed and fully developed), different mechanisms of runoff generation (quick flow on roads and slow flow through subsurface) and different watershed landscape characteristics (wetlands, hillslopes) . Measurements of the Oxygen-18 content of throughfall, stream water, soil water and groundwater in the saturated zone were performed bi-weekly over a period of 2 years. Mean water residence times of the stream water, soil water and groundwater were estimated using Oxygen-18 and Helium-3/Tritium isotopes. There are small but significant differences in the isotopic content of waters in each watershed, along with soil and aquifer properties as a function of the level of urbanization. Longer groundwater residence times (up to more than 2 years) were estimated in wetland zones without direct communication with streams in comparison to hillslope areas (up to more than 1 year). In highly urbanized areas, mixing of natural runoff generation processes with urbanization effects such as the influence of septic plumes results in a complex spectrum of residence times in soil waters and groundwaters. We illustrate the possibilities of using stable isotope measurements to describe small-scale complex runoff generation processes in watersheds.

  3. Distribution characteristics and indicator significance of Dechloranes in multi-matrices at Ny-Ålesund in the Arctic.

    PubMed

    Na, Guangshui; Wei, Wei; Zhou, Shiyao; Gao, Hui; Ma, Xindong; Qiu, Lina; Ge, Linke; Bao, Chenguang; Yao, Ziwei

    2015-02-01

    In recent years, Dechloranes have been widely detected in the environment around the world. However, understanding and knowledge of Dechloranes in remote regions, such as the Arctic, remain lacking. Therefore, the concentrations of 5 Dechloranes in surface seawater, sediment, soil, moss, and dung collected from Ny-Ålesund in the Arctic were measured with the concentrations 93 pg/L, 342, 325, 1.4, and 258 pg/g, respectively, which were much lower than those in Asian and European regions. The mean ratios of anti-Dechlorane Plus (DP) to total DP (ƒanti) in seawater, sediment, soil, moss, dung, and atmospheric samples were 0.36, 0.21, 0.18, 0.27, 0.66, and 0.43, respectively. Results suggested that the main source of DP in seawater, sediment, soil, and moss was long-range atmospheric transport. However, the ratio identified in dung was different, for which the migration behavior of the organism is probably the main source of DP. PMID:25662232

  4. Mixed phase cloud observation in Ny-Alesund, Svalbard: Preliminary results from the May-June 2011 experiment

    NASA Astrophysics Data System (ADS)

    Shiobara, M.; Uchiyama, A.; Yamazaki, A.; Kobayashi, H.

    2011-12-01

    Clouds and aerosols are key elements having the potential to change climate by their radiative effects on the energy balance in the global climate system. In the Arctic, we have been continuing ground-based remote-sensing measurements for clouds and aerosols using a sky-radiometer, a micro-pulse lidar and an all-sky camera in Ny-Alesund (78.9N, 11.9E), Svalbard. In addition to the regular operations, we have performed an intensive observation campaign for mixed phase clouds in May-June 2011. This campaign aimed at low-level clouds to investigate cloud optical and microphysical properties and cloud-aerosol interaction processes in the Arctic, mainly from cloud radiation measurements and active remote-sensing at the surface, and in-situ microphysics measurements at the Norwegian Zeppelin Station located at a 474 meters high mountain-side. The instrumentation for in-situ measurements includes conventional cloud microphysics probes, i.e., DMT CAPS and Gerber PVM-100, and a newly developed cloud particle microscopic imager for cloud water/ice particle size distributions and the effective radius. The Rion KR-12A aerosol particle counter and the TSI 3007 condensation particle counter were placed in the ropeway cabin for measuring particle number and size distribution to see the difference between in-cloud and below-cloud conditions. We will present preliminary results from the in-situ cloud measurements for several days during the May-June 2011 field experiment.

  5. [MAX-DOAS measurements of NO2 column densities and vertical distribution at Ny-Alesund, Arctic during summer].

    PubMed

    Luo, Yu-Han; Sun, Li-Guang; Liu, Wen-Qing; Xie, Pin-Hua; Si, Fu-Qi; Zhou, Hai-Jin

    2012-09-01

    The multi-axis differential optical absorption spectroscopy (MAX-DOAS), one of the remote sensing techniques for trace gases measurements, is sensitive to the lower atmosphere by eliminating the influence of stratosphere retrieved from zenith-sky spectroscopy. Ground-based MAX-DOAS measurements were carried out to observe NO2 at Ny-Alesund, Arctic from 5th Jul to 1st Aug 2011. The differential slant column densities (DSCDs) of NO2 at four off-axis angles showed typical pattern of tropospheric absorbers. Based on the assumption that NO2 was well mixed in 0-1 km of the troposphere, the mean mixing ratio of NO2 during the measurement period was 1.023E11 molec x cm(-3). The fluctuation of NO2 might be related to the fossil fuel combustions and the photochemical reactions. The vertical distribution of NO2 at 0-3 km showed that NO2 was mainly originated from boundary layer of sea surface. PMID:23240391

  6. Oxidation of gaseous elemental mercury to gaseous divalent mercury during 2003 polar sunrise at Ny-Alesund.

    PubMed

    Sprovieri, Francesca; Pirrone, Nicola; Landis, Matthew S; Stevens, Robert K

    2005-12-01

    The springtime phenomenon, termed as the mercury depletion event (MDE), during which elemental gaseous mercury (Hg0) may be converted to a reactive form that accumulates in polar ecosystems, first noted in the Arctic, has now been observed at both poles and results in an important removal pathway for atmospheric mercury. An intensive international springtime mercury experiment was performed at Ny-Alesund, Spitsbergen, from 19 April to 13 May 2003 to study the atmospheric mercury chemistry in the Arctic environment and, in particular, the MDEs which occurred in the arctic boundary layer after polar sunrise. Automated ambient measurements of Hg0, divalent reactive gaseous mercury (RGM) and fine particulate mercury (<2.5 microm) (Hg(p)) were made at the Zeppelin Mountain Station (ZMS). During the experiment mercury concentrations in the lower atmosphere varied in synchrony with ozone levels throughout the Spring. Hg0 concentrations ranged from background levels (approximately 1.6 ng m(-3)) to undetectable values (<0.1 ng m(-3)) during the first and major MDE, while RGM data showed an opposite trend during the sampling period with concentrations increasing dramatically to a peak of 230 pg m(-3), synchronous with the depletion of Hg0. The results of a meteorological transport analysis indicate the MDEs observed at ZMS were primarily due to air masses being transported in from open water areas in the Arctic Ocean that were already depleted of Hg0 when they arrived and not due to in-situ oxidation mechanisms.

  7. Perceptions of HIV Risk and Explanations of Sexual Risk Behavior Offered by Heterosexual Black Male Barbershop Patrons in Brooklyn, NY

    PubMed Central

    Taylor, Tonya N.; Joseph, Michael; Henny, Kirk D.; Pinto, Angelo R.; Agbetor, Francis; Camilien, Brignel; Williams, Kim M.; Browne, Ruth C.; White, Marilyn; Gousse, Yolene; Brown, Humberto; Taylor, Raekiela D.; Wilson, Tracey E.

    2015-01-01

    To describe HIV risk factors among adult heterosexual Black men recruited from four barbershops located in high HIV seroprevalent neighborhoods of Brooklyn, NY. Data on HIV-risk related behaviors and other characteristics were collected from barbershop clients. All participants (n=60) completed brief risk assessments; and a subset (n=22) also completed focus groups and/or individual interviews. Of the subset of 22 men, 68% were US born, 59% had been in jail/prison, 32% were unemployed; and during the 3 months before the interviews, 68% reported at least two partners and 45% reported unprotected vaginal or anal sex with two or more women. Emergent themes included: 1) the psychological function of multiple partnerships; 2) calculated risk taking regarding condom use; 3) the role of emotional attachment and partner trust in condom use; 4) low perceived HIV risk and community awareness; and 5) lack of relationship between HIV testing and safer sex practices. Interventions among heterosexual Black men should focus not only on increasing HIV awareness and reducing sexual risk, but also on contextual and interpersonal factors that influence sexual risk. PMID:25699198

  8. Antibody responses to NY-ESO-1 in primary breast cancer identify a subtype target for immunotherapy.

    PubMed

    Hamaï, Ahmed; Duperrier-Amouriaux, Karine; Pignon, Pascale; Raimbaud, Isabelle; Memeo, Lorenzo; Colarossi, Cristina; Canzonieri, Vincenzo; Perin, Tiziana; Classe, Jean-Marc; Campone, Mario; Jézéquel, Pascal; Campion, Loïc; Ayyoub, Maha; Valmori, Danila

    2011-01-01

    The highly immunogenic human tumor antigen NY-ESO-1 (ESO) is a target of choice for anti-cancer immune therapy. In this study, we assessed spontaneous antibody (Ab) responses to ESO in a large cohort of patients with primary breast cancer (BC) and addressed the correlation between the presence of anti-ESO Ab, the expression of ESO in the tumors and their characteristics. We found detectable Ab responses to ESO in 1% of the patients. Tumors from patients with circulating Ab to ESO exhibited common characteristics, being mainly hormone receptor (HR)⁻ invasive ductal carcinomas of high grade, including both HER2⁻ and HER2⁺ tumors. In line with these results, we detected ESO expression in 20% of primary HR⁻ BC, including both ESO Ab⁺ and Ab⁻ patients, but not in HR⁺ BC. Interestingly, whereas expression levels in ESO⁺ BC were not significantly different between ESO Ab⁺ and Ab⁻ patients, the former had, in average, significantly higher numbers of tumor-infiltrated lymph nodes, indicating that lymph node invasion may be required for the development of spontaneous anti-tumor immune responses. Thus, the presence of ESO Ab identifies a tumor subtype of HR⁻ (HER2⁻ or HER2⁺) primary BC with frequent ESO expression and, together with the assessment of antigen expression in the tumor, may be instrumental for the selection of patients for whom ESO-based immunotherapy may complement standard therapy.

  9. Demographic Profiles, Mercury, Selenium, and Omega-3 Fatty Acids in Avid Seafood Consumers on Long Island, NY.

    PubMed

    Monastero, Rebecca; Karimi, Roxanne; Silbernagel, Susan; Meliker, Jaymie

    2016-02-01

    Seafood consumption is known to confer nutritional benefits and risks from contaminant exposure. Avid seafood consumers are neither well-characterized with regard to their demographic profile nor their underlying risk-benefit profile. Contaminants [e.g., mercury (Hg)] and nutrients [e.g., selenium (Se), omega-3 fatty acids] are prevalent in some seafood. Participants (N = 285) recruited on Long Island, NY, completed food frequency and health questionnaires and received blood draws analyzed for Hg, omega-3s, and Se. Participants were categorized based on frequency and type of seafood consumption. Logistic regression analyses evaluated relationships between seafood consumption and demographics, and were age- and sex-adjusted. t tests assessed relationships between seafood consumption patterns and biomarkers Hg, omega-3s, and Se. Consumption of both tuna and salmon was associated with older age: those aged 55-75 and over 75 years old were more likely than participants aged 18-34 to eat tuna and salmon (OR 2.27; 95% CI 1.05, 4.89 and OR 3.67; 95% CI 1.20, 11.20, respectively). Males were less likely than females to eat fish other than tuna or salmon (OR 0.58; 95% CI 0.34, 0.97). Caucasians were more likely to consume tuna (OR 0.31; 95% CI 0.10, 0.96) or salmon and tuna (OR 0.34; 95% CI 0.12, 0.91), while non-Caucasians were more likely to consume other fish types (OR 2.73; 95% CI 1.45, 5.12). Total blood Hg was associated with weekly consumption of any type of fish (p = 0.01) and with salmon and tuna consumption (p = 0.01). Salmon was associated with plasma omega-3s (p = 0.01). Se was not associated with fish intake categories. Risk communicators can use these findings to influence seafood preferences of different demographic groups.

  10. Tectonically controlled Quaternary intracontinental fluvial sequence development in the Nyírség-Pannonian Basin, Hungary

    NASA Astrophysics Data System (ADS)

    Püspöki, Z.; Demeter, G.; Tóth-Makk, Á.; Kozák, M.; Dávid, Á.; Virág, M.; Kovács-Pálffy, P.; Kónya, P.; Gyuricza, Gy.; Kiss, J.; McIntosh, R. W.; Forgács, Z.; Buday, T.; Kovács, Z.; Gombos, T.; Kummer, I.

    2013-01-01

    The Quaternary fluvial succession of the Nyírség (NE Hungary), a proximal sub-basin of 4000 km2 in the intracontinental Pannonian Basin, was studied based on log facies analysis. Regional mapping of sequences was established by analysis of fully cored boreholes and high scale local correlations in densely drilled areas. The age of the sequences was determined by correlating the magnetic susceptibility (MS) record of the fully cored boreholes with that of the reference Hungarian boreholes dated paleomagnetically (Dévaványa-1 and Vésztő-1 in Cooke et al., 1979). To give the Hungarian data global perspective they were correlated to the MS curve of the Chinese Loess (Ding et al., 2005) that are in turn correlated with the Marine Isotope Record (Lisiecki and Raymo, 2005; Gibbard and Cohen, 2008). The ages of the mapped sequence boundaries are 2.62, 2.26, 2.12, 1.22, 1.04, 0.58 and 0.34 Ma respectively and can be related to the transitions from cool to warm conditions. Regional unconformities at 2.26-2.12, 1.22 and 0.58 Ma also coincide with activity maxima of the radiometrically dated Quaternary volcanism. This high frequency of climatically controlled erosive sequence boundaries in the structurally active periods indicates that the sedimentary record of climatic erosion is better expressed in times when structural changes generate instability in the drainage network. The occurrence of packages of regional unconformities in relation to volcanic activity enables the geochronological dating of episodes in the Quaternary compression of the Carpathian-Pannonian region. The role of tectonic control on the climatically induced changes in the drainage network has been explained by a structural development model based on seismic, gravity and magnetic data. The changes in the local paleohydrology were triggered by a compression related elevation of the basement and the associated occurrence of a local transtension-related subsidence.

  11. Hurricane Sandy deposits on Fire Island, NY: Using washover deposit stratigraphy to understand sediment transport during large storms.

    NASA Astrophysics Data System (ADS)

    La Selle, S.; Gelfenbaum, G. R.; Jaffe, B. E.; Costa, P. J. M.; Lunghino, B.; Bellanova, P.

    2015-12-01

    By examining coastal deposits from modern inundation events, a number of criteria have been developed to help distinguish between onshore storm and tsunami deposits. The presence of sedimentary structures such as planar lamination and foreset beds in storm deposits suggest a dominance of bedload transport, although some tsunami deposits also contain these structures. The presence of normally graded beds in many tsunami deposits is indicative of the dominance of suspended load transport. Understanding how the stratigraphy in modern event deposits develops during inundation and post-deposition modification improves our ability to differentiate between tsunami and storm deposits in the geologic record. We investigated Hurricane Sandy washover deposits on Fire Island, NY a year after the storm in order to describe deposit stratigraphy on sandy barrier islands. Stratigraphic descriptions from trenches, bulk sediment samples, and push cores were collected. So far, grain size and microtextural analyses have been performed on bulk sediment samples. Deposits ranged from 1-100 cm thick and consist of very well-sorted, fine to coarse sand. Elevation profiles of washover features were measured using Differential GPS. Comparisons of GPS elevations to Lidar data from pre- and post-Sandy surveys show that at some locations, the upper 10-40 cm of the deposit had been reworked in the year following Hurricane Sandy. However, there were still areas where the deposit was almost entirely intact, consisting of massive sand layers near the base, overlain by laminated layers. We hypothesize that the lower sand layers represent deposition from overwash carrying sand in suspension that may have occurred during dune breaching. The upper laminated layers are indicative of deposition from bedload transport by successive storm waves. Detailed analyses of vertical grainsize trends will help determine if the lower unstratified sand layers were deposited by one or multiple flows.

  12. The route to MBxNyCz molecular wheels: II. Results using accurate functionals and basis sets

    NASA Astrophysics Data System (ADS)

    Güthler, A.; Mukhopadhyay, S.; Pandey, R.; Boustani, I.

    2014-04-01

    Applying ab initio quantum chemical methods, molecular wheels composed of metal and light atoms were investigated. High quality basis sets 6-31G*, TZPV, and cc-pVTZ as well as exchange and non-local correlation functionals B3LYP, BP86 and B3P86 were used. The ground-state energy and structures of cyclic planar and pyramidal clusters TiBn (for n = 3-10) were computed. In addition, the relative stability and electronic structures of molecular wheels TiBxNyCz (for x, y, z = 0-10) and MBnC10-n (for n = 2 to 5 and M = Sc to Zn) were determined. This paper sustains a follow-up study to the previous one of Boustani and Pandey [Solid State Sci. 14 (2012) 1591], in which the calculations were carried out at the HF-SCF/STO3G/6-31G level of theory to determine the initial stability and properties. The results show that there is a competition between the 2D planar and the 3D pyramidal TiBn clusters (for n = 3-8). Different isomers of TiB10 clusters were also studied and a structural transition of 3D-isomer into 2D-wheel is presented. Substitution boron in TiB10 by carbon or/and nitrogen atoms enhances the stability and leads toward the most stable wheel TiB3C7. Furthermore, the computations show that Sc, Ti and V at the center of the molecular wheels are energetically favored over other transition metal atoms of the first row.

  13. Novel L-cysteine-dependent maleylpyruvate isomerase in the gentisate pathway of Paenibacillus sp. strain NyZ101.

    PubMed

    Liu, Ting-Ting; Zhou, Ning-Yi

    2012-08-01

    Glutathione- and mycothiol-dependent maleylpyruvate isomerases are known to be involved, respectively, in gentisate catabolism in Gram-negative and high G+C Gram-positive strains. In the present study, a low-G+C Gram-positive Paenibacillus sp. strain, NyZ101, was isolated and shown to degrade 3-hydroxybenzoate via gentisate. A 6.5-kb fragment containing a conserved region of gentisate 1,2-dioxygenase genes was cloned and sequenced, and four genes (bagKLIX) were shown to encode the enzymes involved in the catabolism to central metabolites of 3-hydroxybenzoate via gentisate. The Bag proteins share moderate identities with the reported enzymes in the 3-hydroxybenzoate catabolism, except BagL that had no obvious homology with any functionally characterized proteins. Recombinant BagL was purified to homogeneity as a His-tagged protein and likely a dimer by gel filtration. BagL was demonstrated to be a novel thiol-dependent maleylpyruvate isomerase catalyzing the isomerization of maleylpyruvate to fumarylpyruvate with L-cysteine, cysteinylglycine, or glutathione, as its cofactor. The K(m) values of these three thiols for BagL were 15.5, 8.4, and 552 μM, respectively. Since cysteine and coenzyme A were reported to be abundant in low-G+C Gram-positive strains, BagL should utilize L-cysteine as its physiological cofactor in vivo. The addition of Ni(2+) increased BagL activity, and site-directed mutagenesis experiments indicated that three conserved histidines in BagL were associated with binding to Ni(2+) ion and were necessary for its enzyme activity. BagL is the first characterized L-cysteine-dependent catabolic enzyme in microbial metabolism and is likely a new and distinct member of DinB family, with a four-helix-bundle topology, as deduced by sequence analysis and homology modeling.

  14. Trypanosoma cruzi adjuvants potentiate T cell-mediated immunity induced by a NY-ESO-1 based antitumor vaccine.

    PubMed

    Junqueira, Caroline; Guerrero, Ana Tereza; Galvão-Filho, Bruno; Andrade, Warrison A; Salgado, Ana Paula C; Cunha, Thiago M; Ropert, Catherine; Campos, Marco Antônio; Penido, Marcus L O; Mendonça-Previato, Lúcia; Previato, José Oswaldo; Ritter, Gerd; Cunha, Fernando Q; Gazzinelli, Ricardo T

    2012-01-01

    Immunological adjuvants that induce T cell-mediate immunity (TCMI) with the least side effects are needed for the development of human vaccines. Glycoinositolphospholipids (GIPL) and CpGs oligodeoxynucleotides (CpG ODNs) derived from the protozoa parasite Trypanosoma cruzi induce potent pro-inflammatory reaction through activation of Toll-Like Receptor (TLR)4 and TLR9, respectively. Here, using mouse models, we tested the T. cruzi derived TLR agonists as immunological adjuvants in an antitumor vaccine. For comparison, we used well-established TLR agonists, such as the bacterial derived monophosphoryl lipid A (MPL), lipopeptide (Pam3Cys), and CpG ODN. All tested TLR agonists were comparable to induce antibody responses, whereas significant differences were noticed in their ability to elicit CD4(+) T and CD8(+) T cell responses. In particular, both GIPLs (GTH, and GY) and CpG ODNs (B344, B297 and B128) derived from T. cruzi elicited interferon-gamma (IFN-γ) production by CD4(+) T cells. On the other hand, the parasite derived CpG ODNs, but not GIPLs, elicited a potent IFN-γ response by CD8(+) T lymphocytes. The side effects were also evaluated by local pain (hypernociception). The intensity of hypernociception induced by vaccination was alleviated by administration of an analgesic drug without affecting protective immunity. Finally, the level of protective immunity against the NY-ESO-1 expressing melanoma was associated with the magnitude of both CD4(+) T and CD8(+) T cell responses elicited by a specific immunological adjuvant.

  15. Temporal Variation in the Stable Isotopic Composition of Water and Sediment in Seneca Lake, NY (USA): Implications for Paleoclimate Reconstructions

    NASA Astrophysics Data System (ADS)

    Curtin, T.; Finkelstein, D. B.; Gunn, P.

    2014-12-01

    Paleoclimatic studies often use stratigraphic changes in the oxygen isotope composition (δ18O) of lacustrine carbonate to infer changes in water temperature and/or the δ18O of lake water. To better understand the environmental factors that influence the isotopic composition of lacustrine carbonates and the assumptions that need to be made to infer past changes in climate, we undertook a four-year study of the water chemistry and sediment trap material in Seneca Lake (NY, USA), a large, monomictic, glacial, hardwater lake. Sediment trap material collected weekly between May 2009 and 2013 together with isotopic monitoring of surface and bottom water allow for analysis of the controls on calcite precipitation in the lake. We show that calcite mainly accumulates in traps July through September when the lake water column is stratified and epilimnetic water temperature exceeds 20°C. Up to ~70% of the sediment is comprised of calcite during summer and only ~10% of the sediment is calcite during autumn, winter, and spring. The δ18O of Seneca Lake epilimnetic water varies by only ~0.6 ‰ throughout the year whereas the δ18O of bulk carbonate varies by as much as 2.4‰. As calcite precipitates in the eplimnion, the δ18O declines. Likely due to the large volume and residence time of water, the δ18O of Seneca Lake water appears to track changes in temperature. Our temperature reconstruction using the δ18O of calcite and epilimnetic lake water reveals that calcite was a reasonable proxy for lake surface temperature from July through September. During the remainder of the year, reconstructed temperatures exceed actual temperatures by as much as 18°C. Sediment resuspension (including calcite) during isothermal conditions may explain why calcite is a poor predictor of lake surface temperature in late autumn though spring. The δ18O of calcite in this lake records summer temperatures rather than year-round conditions.

  16. Definition of key variables for the induction of optimal NY-ESO-1-specific T cells in HLA transgene mice.

    PubMed

    Johannsen, Alexandre; Genolet, Raphaël; Legler, Daniel F; Luther, Sanjiv A; Luescher, Immanuel F

    2010-09-15

    An attractive treatment of cancer consists in inducing tumor-eradicating CD8(+) CTL specific for tumor-associated Ags, such as NY-ESO-1 (ESO), a strongly immunogenic cancer germ line gene-encoded tumor-associated Ag, widely expressed on diverse tumors. To establish optimal priming of ESO-specific CTL and to define critical vaccine variables and mechanisms, we used HLA-A2/DR1 H-2(-/-) transgenic mice and sequential immunization with immunodominant DR1- and A2-restricted ESO peptides. Immunization of mice first with the DR1-restricted ESO(123-137) peptide and subsequently with mature dendritic cells (DCs) presenting this and the A2-restriced ESO(157-165) epitope generated abundant, circulating, high-avidity primary and memory CD8(+) T cells that efficiently killed A2/ESO(157-165)(+) tumor cells. This prime boost regimen was superior to other vaccine regimes and required strong Th1 cell responses, copresentation of MHC class I and MHC class II peptides by the same DC, and resulted in upregulation of sphingosine 1-phosphate receptor 1, and thus egress of freshly primed CD8(+) T cells from the draining lymph nodes into circulation. This well-defined system allowed detailed mechanistic analysis, which revealed that 1) the Th1 cytokines IFN-gamma and IL-2 played key roles in CTL priming, namely by upregulating on naive CD8(+) T cells the chemokine receptor CCR5; 2) the inflammatory chemokines CCL4 (MIP-1beta) and CCL3 (MIP-1alpha) chemoattracted primed CD4(+) T cells to mature DCs and activated, naive CD8(+) T cells to DC-CD4 conjugates, respectively; and 3) blockade of these chemokines or their common receptor CCR5 ablated priming of CD8(+) T cells and upregulation of sphingosine 1-phosphate receptor 1. These findings provide new opportunities for improving T cell cancer vaccines.

  17. 'Til the Needle Breaks

    ERIC Educational Resources Information Center

    Hawkins, B. Denise

    2011-01-01

    After more than half a century, the music of Motown not only thrives, it transcends generations. The iconic sound of Motown has led a handful of scholars to write, teach, lecture and share the music, history and business of Motown on their campuses. In its golden age, from 1959 to 1972, the sound Berry Gordy pioneered at Motown Records in Detroit…

  18. Salicylic acid is an indispensable component of the Ny-1 resistance-gene-mediated response against Potato virus Y infection in potato

    PubMed Central

    Baebler, Š.; Witek, K.; Gruden, K.; Hennig, J.

    2014-01-01

    The purpose of the study was to investigate the role of salicylic acid (SA) signalling in Ny-1-mediated hypersensitive resistance (HR) of potato (Solanum tuberosum L.) to Potato virus Y (PVY). The responses of the Ny-1 allele in the Rywal potato cultivar and transgenic NahG-Rywal potato plants that do not accumulate SA were characterized at the cytological, biochemical, transcriptome, and proteome levels. Analysis of noninoculated and inoculated leaves revealed that HR lesions started to develop from 3 d post inoculation and completely restricted the virus spread. At the cytological level, features of programmed cell death in combination with reactive oxygen species burst were observed. In response to PVY infection, SA was synthesized de novo. The lack of SA accumulation in the NahG plants led to the disease phenotype due to unrestricted viral spreading. Grafting experiments show that SA has a critical role in the inhibition of PVY spreading in parenchymal tissue, but not in vascular veins. The whole transcriptome analysis confirmed the central role of SA in orchestrating Ny-1-mediated responses and showed that the absence of SA leads to significant changes at the transcriptome level, including a delay in activation of expression of genes known to participate in defence responses. Moreover, perturbations in the expression of hormonal signalling genes were detected, shown as a switch from SA to jasmonic acid/ethylene signalling. Viral multiplication in the NahG plants was accompanied by downregulation of photosynthesis genes and activation of multiple energy-producing pathways. PMID:24420577

  19. Induction of antigen-specific immunity with a vaccine targeting NY-ESO-1 to the dendritic cell receptor DEC-205.

    PubMed

    Dhodapkar, Madhav V; Sznol, Mario; Zhao, Biwei; Wang, Ding; Carvajal, Richard D; Keohan, Mary L; Chuang, Ellen; Sanborn, Rachel E; Lutzky, Jose; Powderly, John; Kluger, Harriet; Tejwani, Sheela; Green, Jennifer; Ramakrishna, Venky; Crocker, Andrea; Vitale, Laura; Yellin, Michael; Davis, Thomas; Keler, Tibor

    2014-04-16

    Immune-based therapies for cancer are generating substantial interest because of the success of immune checkpoint inhibitors. This study aimed to enhance anticancer immunity by exploiting the capacity of dendritic cells (DCs) to initiate T cell immunity by efficient uptake and presentation of endocytosed material. Delivery of tumor-associated antigens to DCs using receptor-specific monoclonal antibodies (mAbs) in the presence of DC-activating agents elicits robust antigen-specific immune responses in preclinical models. DEC-205 (CD205), a molecule expressed on DCs, has been extensively studied for its role in antigen processing and presentation. CDX-1401 is a vaccine composed of a human mAb specific for DEC-205 fused to the full-length tumor antigen NY-ESO-1. This phase 1 trial assessed the safety, immunogenicity, and clinical activity of escalating doses of CDX-1401 with the Toll-like receptor (TLR) agonists resiquimod (TLR7/8) and Hiltonol (poly-ICLC, TLR3) in 45 patients with advanced malignancies refractory to available therapies. Treatment induced humoral and cellular immunity to NY-ESO-1 in patients with confirmed NY-ESO-1-expressing tumors across various dose levels and adjuvant combinations. No dose-limiting or grade 3 toxicities were reported. Thirteen patients experienced stabilization of disease, with a median duration of 6.7 months (range, 2.4+ to 13.4 months). Two patients had tumor regression (~20% shrinkage in target lesions). Six of eight patients who received immune-checkpoint inhibitors within 3 months after CDX-1401 administration had objective tumor regression. This first-in-human study of a protein vaccine targeting DCs demonstrates its feasibility, safety, and biological activity and provides rationale for combination immunotherapy strategies including immune checkpoint blockade. PMID:24739759

  20. SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade

    PubMed Central

    Xue, Wei; Metheringham, Rachael L.; Brentville, Victoria A.; Gunn, Barbara; Symonds, Peter; Yagita, Hideo; Ramage, Judith M.; Durrant, Lindy G.

    2016-01-01

    ABSTRACT Checkpoint blockade has demonstrated promising antitumor responses in approximately 10–40% of patients. However, the majority of patients do not make a productive immune response to their tumors and do not respond to checkpoint blockade. These patients may benefit from an effective vaccine that stimulates high-avidity T cell responses in combination with checkpoint blockade. We have previously shown that incorporating TRP-2 and gp100 epitopes into the CDR regions of a human IgG1 DNA (ImmunoBody®: IB) results in significant tumor regression both in animal models and patients. This vaccination strategy is superior to others as it targets antigen to antigen-presenting cells and stimulates high-avidity T cell responses. To broaden the application of this vaccination strategy, 16 NY-ESO-1 epitopes, covering over 80% of HLA phenotypes, were incorporated into the IB (SCIB2). They produced higher frequency and avidity T cell responses than peptide vaccination. These T cells were of sufficient avidity to kill NY-ESO-1-expressing tumor cells, and in vivo controlled the growth of established B16-NY-ESO-1 tumors, resulting in long-term survival (35%). When SCIB2 was given in combination with Treg depletion, CTLA-4 blockade or PD-1 blockade, long-term survival from established tumors was significantly enhanced to 56, 67 and 100%, respectively. Translating these responses into the clinic by using a combination of SCIB2 vaccination and checkpoint blockade can only further improve clinical responses. PMID:27471648

  1. Impact of climate warming on snow processes in Ny-Ålesund, a polar maritime site at Svalbard

    NASA Astrophysics Data System (ADS)

    López-Moreno, J. I.; Boike, J.; Sanchez-Lorenzo, A.; Pomeroy, J. W.

    2016-11-01

    The impact of observed changes in air temperature and precipitation from 1969 to 2013 and climate projections for 2050 and 2080 at Ny-Ålesund, an arctic research station on Spitzbergen Island in the Svalbard Archipelago on snow hydrological processes, were analyzed using snow accumulation and ablation algorithms in the physically based Cold Regions Hydrological Modelling platform (CRHM). The climate projections were obtained from phase 5 of the Coupled Model Intercomparison Project (CMIP5), with a focus on the snow-dominated period (October to June). To identify the potential effects of increasing temperature and precipitation, a model sensitivity analysis (1 °C to 5 °C), with and without a 25% increase in precipitation, was run on CRHM snow processes. The results indicated that the greatest observed warming was during the early snow season (October-February), with increases of 0.8 and 0.9 °C decade- 1 for maximum (Tmax) and minimum (Tmin) temperatures, respectively. There was also a significant increase in annual and winter precipitation (24 mm decade- 1). The late snow season (March-June) also had a marked increase in temperature (0.5 and 0.69 °C decade- 1 for Tmax and Tmin respectively), but no significant change in precipitation. These changes lead to a significant increase in the number of days with rainfall rather than snowfall. The sensitivity analysis indicated that mean snow water equivalent snowpack will decrease by 10.2% (early snow season) and 11.1% (late snow season) per degree of increased air temperature. For each degree of temperature increase, the modelled peak snow-water-equivalent (SWE) declined by 6.9%, duration of snowpack declined 11 days, and the number of days with rain increased 43% for the early snow season and 12.8% for the late snow season. A warmer climate also leads to markedly decreased surface snow sublimation and the fraction of snowfall eroded and transported by blowing snow. For most snowpack parameters analyzed, the response

  2. Demographic Profiles, Mercury, Selenium, and Omega-3 Fatty Acids in Avid Seafood Consumers on Long Island, NY.

    PubMed

    Monastero, Rebecca; Karimi, Roxanne; Silbernagel, Susan; Meliker, Jaymie

    2016-02-01

    Seafood consumption is known to confer nutritional benefits and risks from contaminant exposure. Avid seafood consumers are neither well-characterized with regard to their demographic profile nor their underlying risk-benefit profile. Contaminants [e.g., mercury (Hg)] and nutrients [e.g., selenium (Se), omega-3 fatty acids] are prevalent in some seafood. Participants (N = 285) recruited on Long Island, NY, completed food frequency and health questionnaires and received blood draws analyzed for Hg, omega-3s, and Se. Participants were categorized based on frequency and type of seafood consumption. Logistic regression analyses evaluated relationships between seafood consumption and demographics, and were age- and sex-adjusted. t tests assessed relationships between seafood consumption patterns and biomarkers Hg, omega-3s, and Se. Consumption of both tuna and salmon was associated with older age: those aged 55-75 and over 75 years old were more likely than participants aged 18-34 to eat tuna and salmon (OR 2.27; 95% CI 1.05, 4.89 and OR 3.67; 95% CI 1.20, 11.20, respectively). Males were less likely than females to eat fish other than tuna or salmon (OR 0.58; 95% CI 0.34, 0.97). Caucasians were more likely to consume tuna (OR 0.31; 95% CI 0.10, 0.96) or salmon and tuna (OR 0.34; 95% CI 0.12, 0.91), while non-Caucasians were more likely to consume other fish types (OR 2.73; 95% CI 1.45, 5.12). Total blood Hg was associated with weekly consumption of any type of fish (p = 0.01) and with salmon and tuna consumption (p = 0.01). Salmon was associated with plasma omega-3s (p = 0.01). Se was not associated with fish intake categories. Risk communicators can use these findings to influence seafood preferences of different demographic groups. PMID:26318872

  3. Inner-shelf circulation and sediment dynamics on a series of shoreface connected ridges offshore of Fire Island, NY

    USGS Publications Warehouse

    Warner, John C.; List, Jeffrey H.; Schwab, William C.; Voulgaris, George; Armstrong, Brandy N.; Marshall, N

    2014-01-01

    Locations along the inner-continental shelf offshore of Fire Island, NY, are characterized by a series of shoreface connected ridges (SFCRs). These sand ridges have approximate dimensions of 10 km in length, 3 km spacing, up to ~8 m ridge to trough relief, and are oriented obliquely at approximately 30 degrees clockwise from the coastline. Stability analysis from previous studies explains how sand ridges such as these could be formed and maintained by storm-driven flows directed alongshore with a key maintenance mechanism of offshore deflected flows over ridge crests and onshore in the troughs. We examine these processes both with a limited set of idealized numerical simulations and analysis of observational data. Model results confirm that along-shore flows over the SFCRs exhibit offshore veering of currents over the ridge crests and onshore-directed flows in the troughs, and demonstrate the opposite circulation pattern for a reverse wind. To further investigate these maintenance processes, oceanographic instruments were deployed at seven sites on the SFCRs offshore of Fire Island to measure water levels, ocean currents, waves, suspended-sediment concentrations, and bottom stresses from January to April 2012. Data analysis reveals that during storms with winds from the northeast the processes of offshore deflection of currents over ridge crests and onshore in the troughs were observed, and during storm events with winds from the southwest a reverse flow pattern over the ridges occurred. Computations of suspended-sediment fluxes identify periods that are consistent with SFCR maintenance mechanisms. Alongshore winds from the northeast drove fluxes offshore on the ridge crest and onshore in the trough that would tend to promote ridge maintenance. However, alongshore winds from the southwest drove opposite circulations. The wind fields are related to different storm types that occur in the region (low pressure systems, cold fronts, and warm fronts). From the limited

  4. Extreme differentiation in single samples from the olivine-rich zone of the Palisades Sill, NY and NJ

    NASA Astrophysics Data System (ADS)

    Chau, K. X.; Sen, G.; Naslund, H. R.

    2007-12-01

    The Palisades Sill, NY and NJ, has long been the focus of much attention due to its potential contributions to the understanding of igneous differentiation. Many early authors, including N.L. Bowen, thought of the intrusion as an ideal example of crystal settling because of an olivine-rich zone that occurs close to the base of the sill. Later investigations demonstrated that at least three geochemically distinct batches of magma were emplaced at different levels within this body, one of which produced the olivine-rich zone. Here we report the results of our detailed electron probe study of 27 olivine grains, 134 plagioclase grains, and 181 pyroxene grains from four samples of the olivine-rich zone. In one of the rocks plagioclase ranges from An84 (large grain; core) to An0.5 (interstitial plagioclase), whereas olivine ranges from Fo81 - Fo69. These ranges are comparable to our other samples. The plagioclase range is far greater than what is shown by the Skaergaard (An25 - An69) and Bushveld (An30 - An80) intrusions. Though the sill has long been known to be vertically differentiated, such extreme differentiation in individual thumb-sized samples was not expected. The implication is that interstitial melt pockets were so well enclosed (i.e., zero permeability) by the surrounding crystals that they were sealed off from further communication with larger volumes of melt that may have existed at a higher levels in the sill at that time. That is, compaction and associated filter pressing was not efficient in removing the interstitial melt. It is unlikely that such a situation could occur if the olivine-rich zone formed early from a large body of magma because the load pressure of the overlying crystals and melt would be too great to avoid compaction. A better explanation is that the olivine-rich zone was emplaced as a separate magma batch (as suggested by several previous workers based on bulk rock geochemistry) in a mostly solidified magma-crystal mush. In this setting

  5. Dynamics of the methane profile through the water column of meromictic Fayetteville Green Lake, N.Y

    NASA Astrophysics Data System (ADS)

    McClure, B. E.; Havig, J. R.; Sowers, T. A.; Hamilton, T. L.; McCormick, M.; Kump, L. R.

    2013-12-01

    Green Lake in Fayetteville, N.Y. is a meromictic lake with a chemocline approximately 21 meters below the surface where redox chemistry shifts from micro-aerobic to euxinic, and a purple- and green- sulfur bacterial plate is a predominant feature. Historic data, mirrored by our recent (November 2012) high-resolution sampling and analysis, document a monimolimnion methane concentration profile that increases nearly linearly with depth to the bottom sediments. Gas chromatography (GC) and Flame Ionization Detection (FID) analyses reveal concentrations exceeding20 μM CH4 at 30 cm depth in the sediments, lower concentrations ranging from ~5 μM CH4 at 44 m to 1.5 μM CH4 at 21.75 m in the water column, and decreased concentrations with an average of 0.12 μM CH4 from 21 m through the chemocline and the oxic zone, demonstrating a diffusive trend from sediments to the chemocline. However, our findings exhibit a departure from linearity from 21-30 meters in which methane concentrations were higher than expected if the sediments were the sole source of methane. We incubated biomass collected from 24 m (June 2013) to examine the source of this unexpected ';hump' in methane concentrations in the water column. To date, no methane production has been observed. Isotopic analysis for δ 13C in CH4 of seven water samples collected from depths above, below and in the methane ';hump' indicate that methane present in the water column is biogenic. Furthermore, the δ 13C values observed, approximately -100‰, indicate biologically- mediated cycling of methane. δ13C values of dissolved inorganic carbon (DIC) indicate input of oxidized methane. These findings suggest that two sources of methane with similar isotopic compositions exist, one diffusing from lake-bottom sediments and the other laterally injected from seeps at or near the chemocline, with consumption near the base of the chemocline. Coupled geochemical analyses show that sulfide and ammonia exhibit a similar concentration

  6. Characterizing SixNy absorbers and support beams for far-infrared/submillimeter transition-edge sensors

    NASA Astrophysics Data System (ADS)

    Beyer, A. D.; Kenyon, M. E.; Echternach, P. M.; Eom, B.-H.; Bueno, J.; Day, P. K.; Bock, J. J.; Bradford, C. M.

    2010-07-01

    We report on the characterization of SixNy (Si-N) optical absorbers and support beams for transition-edge sensors (TESs). The absorbers and support beams measured are suitable to meet ultra-sensitive noise equivalent power (NEP<=10-19W/√Hz) and effective response time (τ) requirements (τ<=100ms) for space-borne far-infrared( IR)/submillimeter(sub-mm) spectrometers, such as the Background Limited far-Infrared/Sub-mm Spectrograph (BLISS) and the SpicA FAR-infrared Instrument (SAFARI) for the SPace Infrared telescope for Cosmology and Astrophysics (SPICA). The thermal response time (τ0) of an absorber suspended by support beams from a lowtemperature substrate depends on the heat capacity (C) of the absorber and the thermal conductance (G) of the support beams (τ0=C/G). In membrane-isolated TESs for BLISS, the effective response time τ is expected to be a factor of 20 smaller than τ0 because of voltage-biased electrothermal feedback operation and assumption of a reasonable open-loop gain, LI~20. We present design specifications for the arrays of membrane-isolated ultra-sensitive TESs for BLISS. Additionally, we measured G and τ0 for two Si-N noise thermometry device (NTD) architectures made using different fabrication processes: (1) a solid membrane Si-N absorber suspended by thin and long Si-N support beams and (2) a wire-mesh Si-N absorber suspended by long, and even thinner, Si-N support beams. The measurements of G and τ0 were designed to test suitability of the Si-N thermal performance to meet the demands of the two SPICA instruments. The solid membrane NTD architecture is similar to the TES architecture for SAFARI and the mesh membrane NTD is similar to that of BLISS TESs. We report measured values of G and C for several BLISS and SAFARI NTD devices. We observe that the heat capacity of the solid membrane devices can be reduced to the order of 1fJ/K at 65mK for devices that are wet etched by KOH. However, C for these devices is found to be on the order

  7. A statistical study of dayside diffuse aurora observed at Yellow River Station in Ny-Ålesund, Svalbard

    NASA Astrophysics Data System (ADS)

    Han, D.; Hu, Z.; Hu, H.; Yang, H.; Huang, D. H.; Chen, X.

    2014-12-01

    Chinese Yellow River Station (YRS), at Ny-Alesund, Svalbard is one of the few stations that can make longtime optical auroral observation at the cusp latitude in the dayside during the boreal winter season on the Earth. Since November 2003, an optical observation system consisting of three identical all-sky imagers supplied with the narrow band filters centered at 427.8, 557.5 and 630.0nm, has been installed at YRS, and the continuous observations providing us with an unprecedented opportunity to investigate some new properties of dayside aurora. Optical diffuse auroras are normally observed at the sub-aurora latitude in nightside on the ground. However, by using 7-year continuous observations obtained at YRS, we found that the diffuse auroras are very frequently observed on the dayside at the high latitude, i.e., near the cusp. We classified the dayside diffuse aurora into four primary categories. They are (1) Veiling Diffuse Aurora, which has no obvious morphological structure and clear boundaries, sometimes likes a thick cloud cover the entire sky, (2) Patch Diffuse Aurora, which is an aurora patch normally in ~10-100km in width and usually owns pulsating property; (3) Diffuse Arcs, which looks like an arc but is apparently different from the discrete auroral arcs in morphology, and (4) Pulsating Aurora, occurring with sparkling forms. We statistical studied how the occurrence of the four types of dayside diffuse auroras depends on the local time, magnetic activity, and solar wind conditions. We found that the occurrence of each type of dayside diffuse aurora shows a unimodal distribution with magnetic local time and their peaks are mostly appeared near the magnetic local noon. We also noted that ~92.2% of the dayside diffuse auroras is observed under low magnetic activity, i.e., Kp ≤ 3, and the dayside diffuse auroras prefer to be observed under IMF By<0 and Bz>0. Although previous studies suggested that the dayside diffuse aurora should be caused by wave

  8. TiNy Titans: The Role of Dwarf-Dwarf Interactions in Low-mass Galaxy Evolution

    NASA Astrophysics Data System (ADS)

    Stierwalt, S.; Besla, G.; Patton, D.; Johnson, K.; Kallivayalil, N.; Putman, M.; Privon, G.; Ross, G.

    2015-05-01

    We introduce TiNy Titans (TNT), the first systematic study of star formation and the subsequent processing of the interstellar medium in interacting dwarf galaxies. Here we present the first results from a multiwavelength observational program based on a sample of 104 dwarf galaxy pairs selected from a range of environments within the spectroscopic portion of the Sloan Digital Sky Survey and caught in various stages of interaction. The TNT dwarf pairs span mass ratios of M*,1/M*,2 < 10, projected separations <50 kpc, and pair member masses of 7 < log({{M}*}/{{M}⊙ }) < 9.7. The dwarf-dwarf merger sequence, as defined by TNT at z = 0, demonstrates conclusively and for the first time that the star formation enhancement observed for massive galaxy pairs also extends to the dwarf mass range. Star formation is enhanced in paired dwarfs in otherwise isolated environments by a factor of 2.3 (±0.7) at pair separations <50 kpc relative to unpaired analogs. The enhancement decreases with increasing pair separation and extends out to pair separations as large as 100 kpc. Starbursts, defined by Hα EQW >100 Å, occur in 20% of the TNT dwarf pairs, regardless of environment, compared to only 6%-8% of the matched unpaired dwarfs. Starbursts can be triggered throughout the merger (i.e., out to large pair separations) and not just approaching coalescence. Despite their enhanced star formation and triggered starbursts, most TNT dwarf pairs have similar gas fractions relative to unpaired dwarfs of the same stellar mass. Thus, there may be significant reservoirs of diffuse, non-star-forming neutral gas surrounding the dwarf pairs, or the gas consumption timescales may be long in the starburst phase. The only TNT dwarf pairs with low gas fractions (fgas\\lt 0.4) and the only dwarfs, either paired or unpaired, with Hα EQW < 2 Å are found near massive galaxy hosts. We conclude that dwarf-dwarf interactions are significant drivers of galaxy evolution at the low-mass end, but

  9. Production of NY-ESO-1 peptide/DRB1*08:03 tetramers and ex vivo detection of CD4 T-cell responses in vaccinated cancer patients.

    PubMed

    Mizote, Yu; Uenaka, Akiko; Isobe, Midori; Wada, Hisashi; Kakimi, Kazuhiro; Saika, Takashi; Kita, Shoichi; Koide, Yukari; Oka, Mikio; Nakayama, Eiichi

    2014-02-12

    We established CD4 T-cell clones, Mz-1B7, and Ue-21, which recognized the NY-ESO-1 121-138 peptide from peripheral blood mononuclear cells (PBMCs) of an esophageal cancer patient, E-2, immunized with an NY-ESO-1 protein and determined the NY-ESO-1 minimal epitopes. Minimal peptides recognized by Mz-1B7 and Ue-21 were NY-ESO-1 125-134 and 124-134, respectively, both in restriction to DRB1*08:03. Using a longer peptide, 122-135, and five other related peptides, including either of the minimal epitopes recognized by the CD4 T-cell clones, we investigated the free peptide/DR recognition on autologous EBV-B cells as APC and peptide/DR tetramer binding. The results showed a discrepancy between them. The tetramers with several peptides recognized by either Mz-1B7 or the Ue-21 CD4 T-cell clone did not bind to the respective clone. On the other hand, unexpected binding of the tetramer with the peptide not recognized by CD4 T-cells was observed. The clone Mz-1B7 did not recognize the free peptide 122-135 on APC, but the peptide 122-135/DRB1*08:03 tetramer bound to the TCR on those cells. The failure of tetramer production and the unexpected tetramer binding could be due to a subtly modified structure of the peptide/DR tetramer from the structure of the free peptide/DR molecule. We also demonstrated that the NY-ESO-1 123-135/DRB1*08:03 tetramer detected ex vivo CD4 T-cell responses in PBMCs from patients after NY-ESO-1 vaccination in immunomonitoring.

  10. Bacterial diversity and bioprospecting for cold-active lipases, amylases and proteases, from culturable bacteria of kongsfjorden and Ny-alesund, Svalbard, Arctic.

    PubMed

    Srinivas, T N R; Nageswara Rao, S S S; Vishnu Vardhan Reddy, P; Pratibha, M S; Sailaja, B; Kavya, B; Hara Kishore, K; Begum, Z; Singh, S M; Shivaji, S

    2009-11-01

    Culturable bacterial diversity of seven marine sediment samples of Kongsfjorden and a sediment and a soil sample from Ny-Alesund, Svalbard, Arctic was studied. The bacterial abundance in the marine sediments of Kongsfjorden varied marginally (0.5 x 10(3)-1.3 x 10(4) cfu/g sediment) and the bacterial number in the two samples collected from the shore of Ny-Alesund also was very similar (0.6 x 10(4) and 3.4 x 10(4), respectively). From the nine samples a total of 103 bacterial isolates were obtained and these isolates could be grouped in to 47 phylotypes based on the 16S rRNA gene sequence belonging to 4 phyla namely Actinobacteria, Bacilli, Bacteroidetes and Proteobacteria. Representatives of the 47 phylotypes varied in their growth temperature range (4-37 degrees C), in their tolerance to NaCl (0.3-2 M NaCl) and growth pH range (2-11). Representatives of 26 phylotypes exhibited amylase and lipase activity either at 5 or 20 degrees C or at both the temperatures. A few of the representatives exhibited amylase and/or lipase activity only at 5 degrees C. None of the phylotypes exhibited protease activity. Most of the phylotypes (38) were pigmented. Fatty acid profile studies indicated that short chain fatty acids, unsaturated fatty acids, branched fatty acids, the cyclic and the cis fatty acids are predominant in the psychrophilic bacteria.

  11. Evidence of the adoption and implementation of a statewide childhood obesity prevention initiative in the New York State WIC Program: the NY Fit WIC process evaluation.

    PubMed

    Sekhobo, Jackson P; Egglefield, Katherine; Edmunds, Lynn S; Shackman, Gene

    2012-04-01

    Process evaluations are critical in determining whether outcome evaluations are warranted. This study assessed the extent to which a childhood obesity prevention initiative, NY Fit WIC, was adopted and implemented by the New York State Supplemental Nutrition Program for Women, Infants and Children (WIC). Process data came from qualitative telephone interviews of 101 WIC local agency directors, following NY Fit WIC trainings. Activities were summarized and cross-tabulated by target level (i.e. participant, staff, agency or community) and by theoretical construct (i.e. knowledge promotion, skill building, self-efficacy or role modeling). Approximately 528 activities were reported across all WIC agencies. When activities were grouped into similar categories, 123 unique activities were identified. Agencies were more likely to implement physical activity-related activities (67%) than nutrition-related activities (33%). The majority of activities targeted WIC participants (47%) and staff (22%) and focused on skill building/self-efficacy (67%) among participants and on role modeling (61%) among staff. The involvement of all agencies shows a high level of adoption of the initiative. The diverse number of activities suggests that WIC local agencies tailored implementation to match their resources and clients' needs as planned. These results suggest that an outcome evaluation is warranted to determine whether meaningful behavioral changes occurred among WIC participants and staff.

  12. Short-term variations in surface UV-B irradiance and total ozone column at Ny-Ålesund during the QAARC campaign

    NASA Astrophysics Data System (ADS)

    Petkov, Boyan; Vitale, Vito; Gröbner, Julian; Hülsen, Gregor; De Simone, Sara; Gallo, Veronica; Tomasi, Claudio; Busetto, Maurizio; Barth, Vigdis Lonar; Lanconelli, Christian; Mazzola, Mauro

    2012-05-01

    Diurnal variations in total ozone column were observed to exceed 40 DU during the QAARC campaign of late spring 2009 held at the Ny-Ålesund Arctic station in Svalbard. They were correlated to corresponding oscillations of surface UV-B irradiance, assuming that the strong dependence between these two variables ensures the reliability of registered total ozone oscillations. To make the comparison, an approach is proposed that allows the retrieval of diurnal variability in UV-B irradiance due to total ozone column changes, for application to the data collected during the campaign. The UV irradiance features observed with the reference spectroradiometer over a 7-day period were analyzed together with the corresponding ozone column data-sets, simultaneously obtained using two different devices. Power curves were found to provide the best-fit solutions for the retrieved UV-B variations, examined as a function of total ozone variations. Such approximations are characterized by regression coefficients of about - 0.94 and by the exponent named in earlier studies as the radiation amplification factor (RAF), which was found to vary between 0.79 and 2.49. These RAF evaluations are in good agreement with previous estimates derived from both field measurements and model evaluations, which confirm the complete dependability of the short-term variations in the ozone column measured at Ny-Ålesund.

  13. Luminescence properties of Ce3+ and Tb3+ co-doped SiOxNy thin films: Prospects for color tunability in silicon-based hosts

    NASA Astrophysics Data System (ADS)

    Ramírez, J. M.; Ruiz-Caridad, A.; Wojcik, J.; Gutierrez, A. M.; Estradé, S.; Peiró, F.; Sanchís, P.; Mascher, P.; Garrido, B.

    2016-03-01

    In this work, the role of the nitrogen content, the annealing temperature, and the sample morphology on the luminescence properties of Ce3+ and Tb3+ co-doped SiOxNy thin films has been investigated. An increasing nitrogen atomic percentage has been incorporated in the host matrix by gradually replacing oxygen with nitrogen during fabrication while maintaining the Si content unaltered, obtaining a sequential variation in the film composition from nearly stoichiometric SiO2 to SiOxNy. The study of rare earth doped single layers has allowed us to identify the parameters that yield an optimum optical performance from Ce3+ and Tb3+ ions. Ce3+ ions proved to be highly sensitive to the annealing temperature and the nitrogen content, showing strong PL emission for relatively low nitrogen contents (from 0 to 20%) and moderate annealing temperatures (800-1000 °C) or under high temperature annealing (1180 °C). Tb3+ ions, on the other hand, displayed a mild dependence on those film parameters. Rare earth co-doping has also been investigated by comparing the luminescence properties of three different approaches: (i) a Ce3+ and Tb3+ co-doped SiOxNy single layer, (ii) a bilayer composed of two SiOxNy single layers doped with either Ce3+ or Tb3+ ions, and (iii) a multilayer composed of a series of either Tb3+ or Ce3+-doped SiOxNy thin films with interleaved SiO2 spacers. Bright green emission and efficient energy transfer from either Ce3+ ions or Ce silicates to Tb3+ ions has been observed in the co-doped single layer as a consequence of the strong ion-ion interaction. On the other hand, independent luminescence from Ce3+ and Tb3+ ions has been observed in the Ce3+ and Tb3+ co-doped bilayer and multilayer, providing a good scenario to develop light emitting devices with wide color tunability by varying the number of deposited films that contain each rare earth dopant. Moreover, the optoelectronic properties of Ce3+- and/or Tb3+-doped thin films have been studied by depositing

  14. Partnership to Enhance Diversity in Marine Geosciences: Holocene Climate and Anthorpogenic Changes from Long Island Sound, NY

    NASA Astrophysics Data System (ADS)

    McHugh, C. M.; Cormier, M.; Marchese, P.; Zheng, Y.; Kohfeld, K.

    2006-12-01

    assistance to attend college; and 3) through our regular teaching schedule for non-geoscience majors. The PIs work at four different institutions from NY metropolitan area: 1) Queens College (QC) from the City University of New York (CUNY.); 2) Queensborough Community College (CUNY), a minority serving college; 3) Lehman College (CUNY), a minority serving college; and 4) Lamont-Doherty Earth Observatory (LDEO), a world-class earth science research institution. External financial support for the program has been secured through the City University of New York Graduate Center through a MAGNET fellowship, Minorities Striving and Pursuing Higher Degrees of Success in Earth Systems Science Professional Development Program 2006, through two industry grants (Entergy Co. and NRG Energy), and in the future, through the availability at competitive rates of local research vessels for day- trips. We anticipate that the program will further gain momentum through partnerships with other City University of New York senior and community colleges, and thanks to word of mouth from those students who participated in the program.

  15. Agreement by and between the County of Rensselaer and the Board of Trustees of Hudson Valley Community College as Co-Employers and the Hudson Valley Community College Non-Teaching Professional Organization, NEA/NY.

    ERIC Educational Resources Information Center

    Hudson Valley Community Coll., Troy, NY.

    This document is comprised of articles of agreement and addenda by and between the County of Rensselaer and the Board of Trustees of Hudson Valley Community College as co-employers and the College Non-Teaching Professional Organization, NEA/NY ("association"). The articles address topics such as determination of employment titles, civil service…

  16. Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination.

    PubMed

    Golnik, Richard; Lehmann, Andrea; Kloetzel, Peter-Michael; Ebstein, Frédéric

    2016-04-15

    The supply of MHC class I-restricted peptides is primarily ensured by the degradation of intracellular proteins via the ubiquitin-proteasome system. Depending on the target and the enzymes involved, ubiquitination is a process that may dramatically vary in terms of linkages, length, and attachment sites. Here we identified the unique lysine residue at position 124 of the NY-ESO-1 cancer/testis antigen as the acceptor site for the formation of canonical Lys-48-linkages. Interestingly, a lysine-less form of NY-ESO-1 was as efficient as its wild-type counterpart in supplying the HLA-A*0201-restricted NY-ESO-1157-165 antigenic peptide. In fact, we show that the regulation of NY-ESO-1 processing by the ubiquitin receptors Rpn10 and Rpn13 as a well as by the standard and immunoproteasome is governed by non-canonical ubiquitination on non-lysine sites. In summary, our data underscore the significance of atypical ubiquitination in the modulation of MHC class I antigen processing.

  17. Detection and Tracking of NY-ESO-1-Specific CD8+ T Cells by High-Throughput T Cell Receptor β (TCRB) Gene Rearrangements Sequencing in a Peptide-Vaccinated Patient.

    PubMed

    Miyai, Manami; Eikawa, Shingo; Hosoi, Akihiro; Iino, Tamaki; Matsushita, Hirokazu; Isobe, Midori; Uenaka, Akiko; Udono, Heiichiro; Nakajima, Jun; Nakayama, Eiichi; Kakimi, Kazuhiro

    2015-01-01

    Comprehensive immunological evaluation is crucial for monitoring patients undergoing antigen-specific cancer immunotherapy. The identification and quantification of T cell responses is most important for the further development of such therapies. Using well-characterized clinical samples from a high responder patient (TK-f01) in an NY-ESO-1f peptide vaccine study, we performed high-throughput T cell receptor β-chain (TCRB) gene next generation sequencing (NGS) to monitor the frequency of NY-ESO-1-specific CD8+ T cells. We compared these results with those of conventional immunological assays, such as IFN-γ capture, tetramer binding and limiting dilution clonality assays. We sequenced human TCRB complementarity-determining region 3 (CDR3) rearrangements of two NY-ESO-1f-specific CD8+ T cell clones, 6-8L and 2F6, as well as PBMCs over the course of peptide vaccination. Clone 6-8L possessed the TCRB CDR3 gene TCRBV11-03*01 and BJ02-01*01 with amino acid sequence CASSLRGNEQFF, whereas 2F6 possessed TCRBV05-08*01 and BJ02-04*01 (CASSLVGTNIQYF). Using these two sequences as models, we evaluated the frequency of NY-ESO-1-specific CD8+ T cells in PBMCs ex vivo. The 6-8L CDR3 sequence was the second most frequent in PBMC and was present at high frequency (0.7133%) even prior to vaccination, and sustained over the course of vaccination. Despite a marked expansion of NY-ESO-1-specific CD8+ T cells detected from the first through 6th vaccination by tetramer staining and IFN-γ capture assays, as evaluated by CDR3 sequencing the frequency did not increase with increasing rounds of peptide vaccination. By clonal analysis using 12 day in vitro stimulation, the frequency of B*52:01-restricted NY-ESO-1f peptide-specific CD8+ T cells in PBMCs was estimated as only 0.0023%, far below the 0.7133% by NGS sequencing. Thus, assays requiring in vitro stimulation might be underestimating the frequency of clones with lower proliferation potential. High-throughput TCRB sequencing using NGS

  18. Effects of an intense, high-frequency laser field on bound states in Ga1 − xInxNyAs1 − y/GaAs double quantum well

    PubMed Central

    2012-01-01

    Within the envelope function approach and the effective-mass approximation, we have investigated theoretically the effect of an intense, high-frequency laser field on the bound states in a GaxIn1 − xNyAs1 − y/GaAs double quantum well for different nitrogen and indium mole concentrations. The laser-dressed potential, bound states, and squared wave functions related to these bound states in Ga1 − xInxNyAs1 − y/GaAs double quantum well are investigated as a function of the position and laser-dressing parameter. Our numerical results show that both intense laser field and nitrogen (indium) incorporation into the GaInNAs have strong influences on carrier localization. PMID:23113959

  19. High-level expression of cancer/testis antigen NY-ESO-1 and human granulocyte-macrophage colony-stimulating factor in dendritic cells with a bicistronic retroviral vector.

    PubMed

    Batchu, R B; Moreno, A M; Szmania, S; Gupta, S K; Zhan, F; Rosen, N; Kozlowski, M; Spencer, T; Spagnoli, G C; Shaughnessy, J; Barlogie, B; Tricot, G; van Rhee, F

    2003-09-20

    Tumor-specific genes delivered to dendritic cells (DCs) have been used for the generation of cytotoxic T cells (CTLs), but their application has been limited on the one hand by low viral titers resulting in low transduction efficiency and poor protein production, and on the other hand by immunogenicity of the selectable marker and poor viability of the DCs. We addressed these limitations by creating a multipurpose master vector (pMV) and cloning the tumor gene NY-ESO-1, which is highly expressed in more than 50% of advanced myeloma patients. pMV was constructed from a Moloney murine leukemia virus (Mo-MuLV)-based retroviral backbone with the following features: (1) an extended packaging signal to achieve high viral titers, (2) a splice acceptor region to facilitate protein production, (3) a nonimmunogenic selectable marker, dihydrofolate reductase-L22Y (DHFR(L22Y)), to exclude the generation of CTLs against the selectable marker, (4) an internal ribosomal entry site between the tumor-specific gene (NY-ESO-1) and the selectable marker DHFR(L22Y) for coexpression of two heterologous gene products from a single bicistronic mRNA, minimizing the possibility of differential expression of these two genes, and (5) human granulocyte-macrophage colony-stimulating factor (hGM-CSF) cDNA driven by the human T-lymphotropic virus promoter to enhance DC function and viability. Recombinant virus of pMV-NY-ESO-1 was generated with vesicular stomatitis virus G envelope protein (VSV-G) in the GP2-293 cell line for efficient transduction. We present evidence that the DC phenotype is unaltered after transduction and that more than 85% of DCs express NY-ESO-1, which secrete approximately 40 ng of GM-CSF per 10(6) DCs. PMID:14503968

  20. Vaccination with NY-ESO-1 protein and CpG in Montanide induces integrated antibody/Th1 responses and CD8 T cells through cross-priming.

    PubMed

    Valmori, Danila; Souleimanian, Naira E; Tosello, Valeria; Bhardwaj, Nina; Adams, Sylvia; O'Neill, David; Pavlick, Anna; Escalon, Juliet B; Cruz, Crystal M; Angiulli, Angelica; Angiulli, Francesca; Mears, Gregory; Vogel, Susan M; Pan, Linda; Jungbluth, Achim A; Hoffmann, Eric W; Venhaus, Ralph; Ritter, Gerd; Old, Lloyd J; Ayyoub, Maha

    2007-05-22

    The use of recombinant tumor antigen proteins is a realistic approach for the development of generic cancer vaccines, but the potential of this type of vaccines to induce specific CD8(+) T cell responses, through in vivo cross-priming, has remained unclear. In this article, we report that repeated vaccination of cancer patients with recombinant NY-ESO-1 protein, Montanide ISA-51, and CpG ODN 7909, a potent stimulator of B cells and T helper type 1 (Th1)-type immunity, resulted in the early induction of specific integrated CD4(+) Th cells and antibody responses in most vaccinated patients, followed by the development of later CD8(+) T cell responses in a fraction of them. The correlation between antibody and T cell responses, together with the ability of vaccine-induced antibodies to promote in vitro cross-presentation of NY-ESO-1 by dendritic cells to vaccine-induced CD8(+) T cells, indicated that elicitation of NY-ESO-1-specific CD8(+) T cell responses by cross-priming in vivo was associated with the induction of adequate levels of specific antibodies. Together, our data provide clear evidence of in vivo cross-priming of specific cytotoxic T lymphocytes by a recombinant tumor antigen vaccine, underline the importance of specific antibody induction for the cross-priming to occur, and support the use of this type of formulation for the further development of efficient cancer vaccines. PMID:17517626

  1. Long-range transport of atmospheric lead reaching Ny-Ålesund: Inter-annual and seasonal variations of potential source areas

    NASA Astrophysics Data System (ADS)

    Bazzano, Andrea; Cappelletti, David; Udisti, Roberto; Grotti, Marco

    2016-08-01

    Atmospheric particulate matter (PM10) was collected at Ny-Ålesund (Svalbard Islands, Norwegian Arctic) during spring and summer from 2010 to 2014 and analysed for lead content, enrichment factor and isotopic composition (208Pb/206Pb and 207Pb/206Pb). It was found that atmospheric lead was mainly of anthropogenic origin and neither its mean concentration, nor its isotopic composition was subjected to significant inter-annual differences (p-value > 0.1). Seasonal differences in both lead content and isotopic compositions occurred (p-value < 0.001), with the exception of 2013 samples. Lead content in spring was higher than in summer. Isotopic analysis indicated that mining and smelting activities in the Rudny Altay region (Central Eurasia), as well as industrial emission in north-eastern North America, were the main sources of atmospheric lead in spring and summer, respectively. During 2013, no significant differences between the two seasons were found (p-value > 0.3), showing a prolonged influence of Eurasian sources also in summer. The results obtained by the Pb isotopic composition were corroborated by a back-trajectory cluster analysis of air-masses reaching the sampling site.

  2. Counseling adolescents about the intrauterine contraceptive device: A comparison of primary care Pediatricians with Family Physicians and Obstetrician-Gynecologists in the Bronx, NY

    PubMed Central

    Rubin, Susan Elizabeth; Cohen, Hillel W; Santelli, John S; McKee, M. Diane

    2015-01-01

    Background The intrauterine device (IUD) is a highly effective contraceptive, yet not all primary care providers (PCPs) counsel adolescents about IUDs. We sought to describe PCPs’ frequency of counseling adolescents about IUDs and identify whether different factors are associated with frequent counseling by Pediatricians compared with Family Physicians and Gynecologists. Methods Surveyed PCPs affiliated with a Bronx, NY academic institution. Main outcome: frequent counseling of female adolescents about IUDs. Results Frequent counseling was lower in Pediatricians compared with Family Physicians and Gynecologists (35.8% and 81.6%, p<.001). Among all PCP types frequent counseling was associated with feeling more competent counseling and managing expected IUD side effects (p=<.001). Other significant variables included inserting IUDs themselves (p <.001, Family Physicians and Gynecologists) or having access to an inserter in their office (p=.04, Pediatricians). Conclusions Correlates of frequent IUD counseling differed according to PCP specialty. Our results suggest that interventions to increase IUD counseling should focus on improving PCPs’ competency around counseling and side effect management as well as increasing access to IUD inserters. PMID:25628297

  3. Building America Case Study: Apartment Compartmentalization with an Aerosol-Based Sealing Process - Queens, NY; Technology Solutions for New and Existing Homes, Energy Efficiency & Renewable Energy (EERE)

    SciTech Connect

    2015-07-01

    Air sealing of building enclosures is a difficult and time-consuming process. Current methods in new construction require laborers to physically locate small and sometimes large holes in multiple assemblies and then manually seal each of them. The innovation demonstrated under this research study was the automated air sealing and compartmentalization of buildings through the use of an aerosolized sealant, developed by the Western Cooling Efficiency Center at University of California Davis.
    CARB sought to demonstrate this new technology application in a multifamily building in Queens, NY. The effectiveness of the sealing process was evaluated by three methods: air leakage testing of overall apartment before and after sealing, point-source testing of individual leaks, and pressure measurements in the walls of the target apartment during sealing. Aerosolized sealing was successful by several measures in this study. Many individual leaks that are labor-intensive to address separately were well sealed by the aerosol particles. In addition, many diffuse leaks that are difficult to identify and treat were also sealed. The aerosol-based sealing process resulted in an average reduction of 71% in air leakage across three apartments and an average apartment airtightness of 0.08 CFM50/SF of enclosure area.

  4. Diversity and distribution of lichen-associated fungi in the Ny-Ålesund Region (Svalbard, High Arctic) as revealed by 454 pyrosequencing.

    PubMed

    Zhang, Tao; Wei, Xin-Li; Zhang, Yu-Qin; Liu, Hong-Yu; Yu, Li-Yan

    2015-10-14

    This study assessed the diversity and distribution of fungal communities associated with seven lichen species in the Ny-Ålesund Region (Svalbard, High Arctic) using Roche 454 pyrosequencing with fungal-specific primers targeting the internal transcribed spacer (ITS) region of the ribosomal rRNA gene. Lichen-associated fungal communities showed high diversity, with a total of 42,259 reads belonging to 370 operational taxonomic units (OTUs) being found. Of these OTUs, 294 belonged to Ascomycota, 54 to Basidiomycota, 2 to Zygomycota, and 20 to unknown fungi. Leotiomycetes, Dothideomycetes, and Eurotiomycetes were the major classes, whereas the dominant orders were Helotiales, Capnodiales, and Chaetothyriales. Interestingly, most fungal OTUs were closely related to fungi from various habitats (e.g., soil, rock, plant tissues) in the Arctic, Antarctic and alpine regions, which suggests that living in association with lichen thalli may be a transient stage of life cycle for these fungi and that long-distance dispersal may be important to the fungi in the Arctic. In addition, host-related factors shaped the lichen-associated fungal communities in this region. Taken together, these results suggest that lichens thalli act as reservoirs of diverse fungi from various niches, which may improve our understanding of fungal evolution and ecology in the Arctic.

  5. Diversity and distribution of lichen-associated fungi in the Ny-Ålesund Region (Svalbard, High Arctic) as revealed by 454 pyrosequencing.

    PubMed

    Zhang, Tao; Wei, Xin-Li; Zhang, Yu-Qin; Liu, Hong-Yu; Yu, Li-Yan

    2015-01-01

    This study assessed the diversity and distribution of fungal communities associated with seven lichen species in the Ny-Ålesund Region (Svalbard, High Arctic) using Roche 454 pyrosequencing with fungal-specific primers targeting the internal transcribed spacer (ITS) region of the ribosomal rRNA gene. Lichen-associated fungal communities showed high diversity, with a total of 42,259 reads belonging to 370 operational taxonomic units (OTUs) being found. Of these OTUs, 294 belonged to Ascomycota, 54 to Basidiomycota, 2 to Zygomycota, and 20 to unknown fungi. Leotiomycetes, Dothideomycetes, and Eurotiomycetes were the major classes, whereas the dominant orders were Helotiales, Capnodiales, and Chaetothyriales. Interestingly, most fungal OTUs were closely related to fungi from various habitats (e.g., soil, rock, plant tissues) in the Arctic, Antarctic and alpine regions, which suggests that living in association with lichen thalli may be a transient stage of life cycle for these fungi and that long-distance dispersal may be important to the fungi in the Arctic. In addition, host-related factors shaped the lichen-associated fungal communities in this region. Taken together, these results suggest that lichens thalli act as reservoirs of diverse fungi from various niches, which may improve our understanding of fungal evolution and ecology in the Arctic. PMID:26463847

  6. Reintroduction of Lake Sturgeon (Acipenser fulvescens) into the St. Regis River, NY: Post-release assessment of habitat use and growth

    USGS Publications Warehouse

    Dittman, Dawn E.; Chalupnicki, Marc A.; Johnson, James H.; Snyder, James

    2015-01-01

    One of the depleted endemic fish species of the Great Lakes, Acipenser fulvescens (Lake Sturgeon), has been the target of extensive conservation efforts. One strategy is reintroduction into historically productive waters. The St. Regis River, NY, represents one such adaptive-management effort, with shared management between New York and the St. Regis Mohawk Tribe. Between 1998 and 2004, a total of 4977 young-of-year Lake Sturgeon were released. Adaptive management requires intermediate progress metrics. During 2004 and 2005, we measured growth, habitat use, and survivorship metrics of the released fish. We captured a total of 95 individuals of all stocked ages. Year-class minimal-survival rates ranged from 0.19–2.1%. The size-at-age and length/biomass relationships were comparable to those reported for juveniles in other Great Lakes waters. These intermediate assessment metrics can provide feedback to resource managers who make restoration-program decisions on a much shorter time-scale than the time-frame in which the ultimate goal of a self-sustaining population can be attained.

  7. Diversity and distribution of lichen-associated fungi in the Ny-Ålesund Region (Svalbard, High Arctic) as revealed by 454 pyrosequencing

    PubMed Central

    Zhang, Tao; Wei, Xin-Li; Zhang, Yu-Qin; Liu, Hong-Yu; Yu, Li-Yan

    2015-01-01

    This study assessed the diversity and distribution of fungal communities associated with seven lichen species in the Ny-Ålesund Region (Svalbard, High Arctic) using Roche 454 pyrosequencing with fungal-specific primers targeting the internal transcribed spacer (ITS) region of the ribosomal rRNA gene. Lichen-associated fungal communities showed high diversity, with a total of 42,259 reads belonging to 370 operational taxonomic units (OTUs) being found. Of these OTUs, 294 belonged to Ascomycota, 54 to Basidiomycota, 2 to Zygomycota, and 20 to unknown fungi. Leotiomycetes, Dothideomycetes, and Eurotiomycetes were the major classes, whereas the dominant orders were Helotiales, Capnodiales, and Chaetothyriales. Interestingly, most fungal OTUs were closely related to fungi from various habitats (e.g., soil, rock, plant tissues) in the Arctic, Antarctic and alpine regions, which suggests that living in association with lichen thalli may be a transient stage of life cycle for these fungi and that long-distance dispersal may be important to the fungi in the Arctic. In addition, host-related factors shaped the lichen-associated fungal communities in this region. Taken together, these results suggest that lichens thalli act as reservoirs of diverse fungi from various niches, which may improve our understanding of fungal evolution and ecology in the Arctic. PMID:26463847

  8. Analysis of Atmospheric Aerosols Collected in an Urban Area in Upstate NY Using Proton Induced X-ray Emission (PIXE) Spectroscopy

    NASA Astrophysics Data System (ADS)

    Smith, Jeremy; Ali, Salina; Nadareski, Benjamin; Safiq, Alexandrea; Yoskowitz, Joshua; Labrake, Scott; Vineyard, Michael

    2013-10-01

    We examined atmospheric aerosol samples collected in Schenectady NY for evidence of pollution. We collected aerosol samples using a nine stage cascade impactor which distributes the particulate matter by aerodynamic size onto 7.5 μm Kapton foils. We then used a 1MV electrostatic Pelletron accelerator to produce a 2.2 MeV proton beam to hit the impacted foils. X-ray intensity versus energy spectra were collected using an Amptek x-ray detector where the x-rays are produced from the proton beam interacting with the sample. This is called PIXE. The elemental composition and concentrations of the elements present in the aerosol samples were determined using a software package called GUPIX. We have found elements ranging from Al to Pb and in particular have found significant amounts of Pb and Br on some of our impacted foils, with a Br/Pb ratio of 0.6 +/- 0.2 which agrees with previous studies. This result suggests the presence of leaded aviation fuel perhaps due to the proximity of the collection site to a small airport with a significant amount of general aviation traffic. Union College.

  9. Radionuclide content of NORM by-products originating from the coal-fired power plant in Oroszlány (Hungary).

    PubMed

    Kardos, R; Sas, Z; Hegedűs, M; Shahrokhi, A; Somlai, J; Kovács, T

    2015-11-01

    At the Thermal Power Plant in Oroszlány (Hungary), a huge amount of by-products have been produced since 1961. In this survey, coal and other by-products were examined (fly ash, bottom ash, fluidised bed bottom ash, gypsum and slurry-type ash). The natural isotopes were determined using an HPGe detector. It was found that the radionuclide content of coal was significantly lower ((226)Ra = 45.3±6.3, (232)Th = 26.3±5.7 and (40)K = 210±21 Bq kg(-1)) than that in the case of ashes other than the fluidised type. The average values of the bottom ash-deposited in the largest quantities-were three times higher than those for coal ((226)Ra = 144±18, (232)Th = 84.3±14 and (40)K = 260±25 Bq kg(-1)). In the case of fractionised bottom ash, the radionuclide content of <0.1 mm was 45 % higher than that of >1.6 mm and the massic radon exhalation of <0.1 mm was approximately four times higher than that above this range.

  10. Polychlorinated biphenyls (PCBs) and polybrominated biphenyl ethers (PBDEs) in environmental samples from Ny-Ålesund and London Island, Svalbard, the Arctic.

    PubMed

    Zhu, Chaofei; Li, Yingming; Wang, Pu; Chen, Zhaojing; Ren, Daiwei; Ssebugere, Patrick; Zhang, Qinghua; Jiang, Guibin

    2015-05-01

    Polychlorinated biphenyls (PCBs) and polybrominated biphenyl ethers (PBDEs) were determined in environmental samples collected from Ny-Ålesund and London Island, Svalbard, the Arctic. Total PCB concentrations (∑25PCBs) varied from 0.57 to 2.52 ng g(-1) dry weight (dw) in soil, 0.30 to 1.16 ng g(-1) dw in plants and 0.56 to 0.98 ng g(-1) dw in reindeer dung. The non-Aroclor congener of CB-11 was predominant in most samples compared to other congeners, accounting for 16.0±9.8% to the ∑25PCBs. The ∑13PBDEs concentrations were 1.7-416, 36.7-495 and 28.1-104 pg g(-1) dw in soil, plants and reindeer dung, respectively. The signature of enantioselective biotransformation was observed in all samples for chiral CB-95, whereas in parts of samples for other chiral PCBs. Bioaccumulation factors (BAFs) in six plant species varied within individual contaminant congeners and plant species, with BAFs less than 1 for ∑PCBs and higher than 1 for ∑PBDEs. BAF values decreased with increasing soil concentrations, suggesting that high background levels in soil restricted the accumulation of these contaminants by plants.

  11. Polychlorinated biphenyls (PCBs) and polybrominated biphenyl ethers (PBDEs) in environmental samples from Ny-Ålesund and London Island, Svalbard, the Arctic.

    PubMed

    Zhu, Chaofei; Li, Yingming; Wang, Pu; Chen, Zhaojing; Ren, Daiwei; Ssebugere, Patrick; Zhang, Qinghua; Jiang, Guibin

    2015-05-01

    Polychlorinated biphenyls (PCBs) and polybrominated biphenyl ethers (PBDEs) were determined in environmental samples collected from Ny-Ålesund and London Island, Svalbard, the Arctic. Total PCB concentrations (∑25PCBs) varied from 0.57 to 2.52 ng g(-1) dry weight (dw) in soil, 0.30 to 1.16 ng g(-1) dw in plants and 0.56 to 0.98 ng g(-1) dw in reindeer dung. The non-Aroclor congener of CB-11 was predominant in most samples compared to other congeners, accounting for 16.0±9.8% to the ∑25PCBs. The ∑13PBDEs concentrations were 1.7-416, 36.7-495 and 28.1-104 pg g(-1) dw in soil, plants and reindeer dung, respectively. The signature of enantioselective biotransformation was observed in all samples for chiral CB-95, whereas in parts of samples for other chiral PCBs. Bioaccumulation factors (BAFs) in six plant species varied within individual contaminant congeners and plant species, with BAFs less than 1 for ∑PCBs and higher than 1 for ∑PBDEs. BAF values decreased with increasing soil concentrations, suggesting that high background levels in soil restricted the accumulation of these contaminants by plants. PMID:25697952

  12. Distribution and transfer pattern of Polychlorinated Biphenyls (PCBs) among the selected environmental media of Ny-Ålesund, the Arctic: As a case study.

    PubMed

    Zhang, Peng; Ge, Linke; Gao, Hui; Yao, Ting; Fang, Xiaodan; Zhou, Chuanguang; Na, Guangshui

    2014-12-15

    Polychlorinated Biphenyls (PCBs) were analyzed in multi-environmental samples collected from Ny-Ålesund, the Arctic to explore their legacy and transfer patterns. PCBs were ubiquitously in the environmental media, within the ranges of 1.73-6.27 and 9.18-141.1 pg m(-3) in vapor and aerosol, 2.76-10.8, 3.09-8.32, 22.5-56.3, 35.4-51.4 and 31.8-39.6 ng g(-1) (dry weight) in soil, sediment, plant, bird guano and reindeer faeces, respectively. The spatial distribution patterns exhibited a general southward decline in soil and sediment from the bay entrance to the inner bay. The concentration ratios of plants to soil inferred that no distinguished selective adsorption of PCBs congeners by plants existed and PCBs were mainly attributed to the air deposition on plant surface. The fugacity ratios among vapor, aerosol and soil indicted that the equilibrium status has not been reached and the net transferring direction was air-soil. PMID:25444625

  13. Readout Durability Improvement of Super-Resolution Near-Field Structure Discs with PtOx-SiO2 Recording and GeNy Interfacial Layers

    NASA Astrophysics Data System (ADS)

    Shima, Takayuki; Yamakawa, Yuzo; Tominaga, Junji

    2007-02-01

    Two dielectric materials were added to a super-resolution near-field optical disc structure (super-RENS) to improve the readout durability. PtOx-SiO2 (Pt8.4Si21.1O70.5) layers showed O2 desorption for temperatures above 200 °C and were usable as write-once recording layers. The GeNy interfacial layers located between the Sb75Te25 and (ZnS)85(SiO2)15 layers suppressed degradation in the reflected light intensity from the disc, i.e., maintained the recorded structure as is, to at least 5× 104 readout cycles using a recorded pattern designed to accelerate disc deterioration. The carrier-to-noise ratio (CNR) obtained for 100-nm marks (laser wavelength: 405 nm, numerical aperture: 0.65) was about 43 dB. It was possible to readout 2.6× 105 cycles before the CNR decreased by 3 dB. This represents an improvement of a factor of 70 over the readout durability of a conventional super-RENS structure.

  14. The influence of accuracy, grid size, and interpolation method on the hydrological analysis of LiDAR derived dems: Seneca Nation of Indians, Irving NY

    NASA Astrophysics Data System (ADS)

    Clarkson, Brian W.

    Light Detection and Ranging (LiDAR) derived Digital Elevation Models (DEMs) provide accurate, high resolution digital surfaces for precise topographic analysis. The following study investigates the accuracy of LiDAR derived DEMs by calculating the Root Mean Square Error (RMSE) of multiple interpolation methods with grid cells ranging from 0.5 to 10-meters. A raster cell with smaller dimensions will drastically increase the amount of detail represented in the DEM by increasing the number of elevation values across the study area. Increased horizontal resolutions have raised the accuracy of the interpolated surfaces and the contours generated from the digitized landscapes. As the raster grid cells decrease in size, the level of detail of hydrological processes will significantly improve compared to coarser resolutions including the publicly available National Elevation Datasets (NEDs). Utilizing a LiDAR derived DEM with the lowest RMSE as the 'ground truth', watershed boundaries were delineated for a sub-basin of the Clear Creek Watershed within the territory of the Seneca Nation of Indians located in Southern Erie County, NY. An investigation of the watershed area and boundary location revealed considerable differences comparing the results of applying different interpretation methods on DEM datasets of different horizontal resolutions. Stream networks coupled with watersheds were used to calculate peak flow values for the 10-meter NEDs and LiDAR derived DEMs.

  15. A novel approach to identify and map kitten clusters using geographic information systems (GIS): a case study from Tompkins County, NY.

    PubMed

    Reading, Anna S; Scarlett, Janet M; Berliner, Elizabeth A

    2014-01-01

    A retrospective study using a geographic information system (GIS) was conducted to capture, map, and analyze intake data of caregiver (owner)-surrendered kittens (aged 0-6 months) to the Society for the Prevention of Cruelty to Animals (SPCA) of Tompkins County, NY, from 2009 to 2011. Addresses of caregiver-surrendered kittens during the study period were mapped (n = 1,017). Mapping and analysis of the resultant data set revealed that the distribution of kittens was nonrandom. Seventeen statistically significant (p = .001) clusters were identified, 1 of which was the SPCA of Tompkins County (due to anonymously surrendered nonhuman animals). The remaining 16 clusters were composed of 52 homes; 27.5% (280/1,017) of the kittens in the data set originated from these 52 homes. The majority of kittens within clusters were surrendered from high-density residential and manufactured residential home parks. Analyzing such clusters using GIS is a novel approach for targeting spay/neuter and educational programs to areas contributing disproportionately to shelter populations. This method may prove useful to help shelters more effectively allocate their limited resources, but further evaluation of this and other targeted approaches is needed to assess the long-term efficacy of such programs.

  16. Aerosol absorption coefficient and Equivalent Black Carbon by parallel operation of AE31 and AE33 aethalometers at the Zeppelin station, Ny Ålesund, Svalbard

    NASA Astrophysics Data System (ADS)

    Eleftheriadis, Konstantinos; Kalogridis, Athina-Cerise; Vratolis, Sterios; Fiebig, Markus

    2016-04-01

    Light absorbing carbon in atmospheric aerosol plays a critical role in radiative forcing and climate change. Despite the long term measurements across the Arctic, comparing data obtained by a variety of methods across stations requires caution. A method for extracting the aerosol absorption coefficient from data obtained over the decades by filter based instrument is still under development. An IASOA Aerosol working group has been initiated to address this and other cross-site aerosol comparison opportunities. Continuous ambient measurements of EBC/light attenuation by means of a Magee Sci. AE-31 aethalometer operating at the Zeppelinfjellet station (474 m asl; 78°54'N, 11°53'E), Ny Ålesund, Svalbard, have been available since 2001 (Eleftheriadis et al, 2009), while a new aethalometer model (AE33, Drinovec et al, 2014) has been installed to operate in parallel from the same inlet since June 2015. Measurements are recorded by a Labview routine collecting all available parameters reported by the two instrument via RS232 protocol. Data are reported at 1 and 10 minute intervals as averages for EBC (μg m-3) and aerosol absorption coefficients (Mm-1) by means of routine designed to report Near Real Time NRT data at the EBAS WDCA database (ebas.nilu.no) Results for the first 6 month period are reported here in an attempt to evaluate comparative performance of the two instruments in terms of their response with respect to the variable aerosol load of light absorbing carbon during the warm and cold seasons found in the high arctic. The application of available conversion schemes for obtaining the absorption coefficient by the two instruments is found to demonstrate a marked difference in their output. During clean periods of low aerosol load (EBC < 30 ng m-3), the two instruments display a better agreement with regression slope for the 880 nm signal between the two at ~ 0.9 compared to a slope at ~ 0.6 during the period of higher absorbing carbon loads (400< EBC<30 ng m

  17. Soil pH is a Key Determinant of Soil Fungal Community Composition in the Ny-Ålesund Region, Svalbard (High Arctic)

    PubMed Central

    Zhang, Tao; Wang, Neng-Fei; Liu, Hong-Yu; Zhang, Yu-Qin; Yu, Li-Yan

    2016-01-01

    This study assessed the fungal community composition and its relationships with properties of surface soils in the Ny-Ålesund Region (Svalbard, High Arctic). A total of thirteen soil samples were collected and soil fungal community was analyzed by 454 pyrosequencing with fungi-specific primers targeting the rDNA internal transcribed spacer (ITS) region. The following eight soil properties were analyzed: pH, organic carbon (C), organic nitrogen (N), ammonium nitrogen (NH4+-N), silicate silicon (SiO42--Si), nitrite nitrogen (NO2--N), phosphate phosphorus (PO43--P), and nitrate nitrogen (NO3--N). A total of 57,952 reads belonging to 541 operational taxonomic units (OTUs) were found. of these OTUs, 343 belonged to Ascomycota, 100 to Basidiomycota, 31 to Chytridiomycota, 22 to Glomeromycota, 11 to Zygomycota, 10 to Rozellomycota, whereas 24 belonged to unknown fungi. The dominant orders were Helotiales, Verrucariales, Agaricales, Lecanorales, Chaetothyriales, Lecideales, and Capnodiales. The common genera (>eight soil samples) were Tetracladium, Mortierella, Fusarium, Cortinarius, and Atla. Distance-based redundancy analysis (db-rda) and analysis of similarities (ANOSIM) revealed that soil pH (p = 0.001) was the most significant factor in determining the soil fungal community composition. Members of Verrucariales were found to predominate in soils of pH 8–9, whereas Sordariales predominated in soils of pH 7–8 and Coniochaetales predominated in soils of pH 6–7. The results suggest the presence and distribution of diverse soil fungal communities in the High Arctic, which can provide reliable data for studying the ecological responses of soil fungal communities to climate changes in the Arctic. PMID:26955371

  18. 2014 iAREA campaign on aerosol in Spitsbergen - Part 2: Optical properties from Raman-lidar and in-situ observations at Ny-Ålesund

    NASA Astrophysics Data System (ADS)

    Ritter, C.; Neuber, R.; Schulz, Alexander; Markowicz, K. M.; Stachlewska, I. S.; Lisok, J.; Makuch, P.; Pakszys, P.; Markuszewski, P.; Rozwadowska, A.; Petelski, T.; Zielinski, T.; Becagli, S.; Traversi, R.; Udisti, R.; Gausa, M.

    2016-09-01

    In this work multi wavelength Raman lidar data from Ny-Ålesund, Spitsbergen have been analysed for the spring 2014 Arctic haze season, as part of the iAREA campaign. Typical values and probability distributions for aerosol backscatter, extinction and depolarisation, the lidar ratio and the color ratio for 4 different altitude intervals within the troposphere are given. These quantities and their dependencies are analysed and the frequency of altitude-dependent observed aerosol events are given. A comparison with ground-based size distribution and chemical composition is performed. Hence the aim of this paper is to provide typical and statistically meaningful properties of Arctic aerosol, which may be used in climate models or to constrain the radiative forcing. We have found that the 2014 season was only moderately polluted with Arctic haze and that sea salt and sulphate were the most dominant aerosol species. Moreover the drying of an aerosol layer after cloud disintegration has been observed. Hardly any clear temporal evolution over the 4 week data set on Arctic haze is obvious with the exception of the extinction coefficient and the lidar ratio, which significantly decreased below 2 km altitude by end April. In altitudes between 2 and 5 km the haze season lasted longer and the aerosol properties were generally more homogeneous than closer to the surface. Above 5 km only few particles were found. The variability of the lidar ratio is discussed. It was found that knowledge of the aerosol's size and shape does not determine the lidar ratio. Contrary to shape and lidar ratio, there is a clear correlation between size and backscatter: larger particles show a higher backscatter coefficient.

  19. 2014 iAREA campaign on aerosol in Spitsbergen - Part 2: Optical properties from Raman-lidar and in-situ observations at Ny-Ålesund

    NASA Astrophysics Data System (ADS)

    Ritter, C.; Neuber, R.; Schulz, Alexander; Markowicz, K. M.; Stachlewska, I. S.; Lisok, J.; Makuch, P.; Pakszys, P.; Markuszewski, P.; Rozwadowska, A.; Petelski, T.; Zielinski, T.; Becagli, S.; Traversi, R.; Udisti, R.; Gausa, M.

    2016-09-01

    In this work multi wavelength Raman lidar data from Ny-Ålesund, Spitsbergen have been analysed for the spring 2014 Arctic haze season, as part of the iAREA campaign. Typical values and probability distributions for aerosol backscatter, extinction and depolarisation, the lidar ratio and the color ratio for 4 different altitude intervals within the troposphere are given. These quantities and their dependencies are analysed and the frequency of altitude-dependent observed aerosol events are given. A comparison with ground-based size distribution and chemical composition is performed. Hence the aim of this paper is to provide typical and statistically meaningful properties of Arctic aerosol, which may be used in climate models or to constrain the radiative forcing. We have found that the 2014 season was only moderately polluted with Arctic haze and that sea salt and sulphate were the most dominant aerosol species. Moreover the drying of an aerosol layer after cloud disintegration has been observed. Hardly any clear temporal evolution over the 4 week data set on Arctic haze is obvious with the exception of the extinction coefficient and the lidar ratio, which significantly decreased below 2 km altitude by end April. In altitudes between 2 and 5 km the haze season lasted longer and the aerosol properties were generally more homogeneous than closer to the surface. Above 5 km only few particles were found. The variability of the lidar ratio is discussed. It was found that knowledge of the aerosol's size and shape does not determine the lidar ratio. Contrary to shape and lidar ratio, there is a clear correlation between size and backscatter: larger particles show a higher backscatter coefficient.

  20. Soil pH is a Key Determinant of Soil Fungal Community Composition in the Ny-Ålesund Region, Svalbard (High Arctic).

    PubMed

    Zhang, Tao; Wang, Neng-Fei; Liu, Hong-Yu; Zhang, Yu-Qin; Yu, Li-Yan

    2016-01-01

    This study assessed the fungal community composition and its relationships with properties of surface soils in the Ny-Ålesund Region (Svalbard, High Arctic). A total of thirteen soil samples were collected and soil fungal community was analyzed by 454 pyrosequencing with fungi-specific primers targeting the rDNA internal transcribed spacer (ITS) region. The following eight soil properties were analyzed: pH, organic carbon (C), organic nitrogen (N), ammonium nitrogen (NH4 (+)-N), silicate silicon (SiO4 (2-)-Si), nitrite nitrogen (NO2 (-)-N), phosphate phosphorus (PO4 (3-)-P), and nitrate nitrogen (NO3 (-)-N). A total of 57,952 reads belonging to 541 operational taxonomic units (OTUs) were found. of these OTUs, 343 belonged to Ascomycota, 100 to Basidiomycota, 31 to Chytridiomycota, 22 to Glomeromycota, 11 to Zygomycota, 10 to Rozellomycota, whereas 24 belonged to unknown fungi. The dominant orders were Helotiales, Verrucariales, Agaricales, Lecanorales, Chaetothyriales, Lecideales, and Capnodiales. The common genera (>eight soil samples) were Tetracladium, Mortierella, Fusarium, Cortinarius, and Atla. Distance-based redundancy analysis (db-rda) and analysis of similarities (ANOSIM) revealed that soil pH (p = 0.001) was the most significant factor in determining the soil fungal community composition. Members of Verrucariales were found to predominate in soils of pH 8-9, whereas Sordariales predominated in soils of pH 7-8 and Coniochaetales predominated in soils of pH 6-7. The results suggest the presence and distribution of diverse soil fungal communities in the High Arctic, which can provide reliable data for studying the ecological responses of soil fungal communities to climate changes in the Arctic.

  1. The potential of a salt-tolerant plant (Distichlis spicata cv. NyPa Forage) to treat effluent from inland saline aquaculture and provide livestock feed on salt-affected farmland.

    PubMed

    Lymbery, Alan J; Kay, Gavin D; Doupé, Robert G; Partridge, Gavin J; Norman, Hayley C

    2013-02-15

    Dryland salinity is a major problem affecting food production from agricultural land in Australia and throughout the world. Although there is much interest in using saline groundwater to grow marine fish on salt-affected farmland, the disposal of nutrient enriched, saline aquaculture effluent is a major environmental problem. We investigated the potential of the salt-tolerant NyPa Forage plant (Distichlis spicata L. Greene var. yensen-4a) to trap nutrients from saline aquaculture effluent and subsequently to provide a fodder crop for livestock. Sub-surface flow wetlands containing NyPa Forage were constructed and their efficacy in removing total nitrogen, ammonia, nitrite/nitrate, total phosphorus and orthophosphate was monitored under different levels of nutrients and salinity. The wetlands removed 60-90% of total nitrogen loads and at least 85% of ammonia, nitrite/nitrate, total phosphorus and orthophosphate loads, with greater efficiency at high nutrient and low salinity levels. The above-ground yield, sodium, crude protein (CP) and in vitro dry matter digestibility (DMD) of NyPa Forage plants were measured after fertilisation with different nutrient levels and cropping at different frequencies. Yield of plants increased with increased nutrient, while nutritive value was greater when nutrients were applied but did not differ among nutrient levels. Yield was not affected by cropping frequency, but nutritive value was greatest when plants were cropped at intervals of 21 or 42 days. At optimum nutrient addition and cropping levels, the plants had a mean CP content of 16.7% and an in vitro DMD of 67.6%, equivalent to an energy value of 9.5 MJ kg(-1). Assuming an equivalent fibre content and voluntary food intake as grass hay, and no accumulation of other toxic minerals, these nutritive values would be sufficient for maintenance or moderate liveweight gains in dry adult sheep or cattle. PMID:23333515

  2. A phase I trial combining decitabine/dendritic cell vaccine targeting MAGE-A1, MAGE-A3 and NY-ESO-1 for children with relapsed or therapy-refractory neuroblastoma and sarcoma.

    PubMed

    Krishnadas, Deepa K; Shusterman, Suzanne; Bai, Fanqi; Diller, Lisa; Sullivan, Janice E; Cheerva, Alexandra C; George, Rani E; Lucas, Kenneth G

    2015-10-01

    Antigen-specific immunotherapy was studied in a multi-institutional phase 1/2 study by combining decitabine (DAC) followed by an autologous dendritic cell (DC)/MAGE-A1, MAGE-A3 and NY-ESO-1 peptide vaccine in children with relapsed/refractory solid tumors. Patients aged 2.5-15 years with relapsed neuroblastoma, Ewing's sarcoma, osteosarcoma and rhabdomyosarcoma were eligible to receive DAC followed by DC pulsed with overlapping peptides derived from full-length MAGE-A1, MAGE-A3 and NY-ESO-1. The primary endpoints were to assess the feasibility and tolerability of this regimen. Each of four cycles consisted of week 1: DAC 10 mg/m(2)/day for 5 days and weeks 2 and 3: DC vaccine once weekly. Fifteen patients were enrolled in the study, of which 10 were evaluable. Generation of DC was highly feasible for all enrolled patients. The treatment regimen was generally well tolerated, with the major toxicity being DAC-related myelosuppression in 5/10 patients. Six of nine patients developed a response to MAGE-A1, MAGE-A3 or NY-ESO-1 peptides post-vaccine. Due to limitations in number of cells available for analysis, controls infected with a virus encoding relevant genes have not been performed. Objective responses were documented in 1/10 patients who had a complete response. Of the two patients who had no evidence of disease at the time of treatment, one remains disease-free 2 years post-therapy, while the other experienced a relapse 10 months post-therapy. The chemoimmunotherapy approach using DAC/DC-CT vaccine is feasible, well tolerated and results in antitumor activity in some patients. Future trials to maximize the likelihood of T cell responses post-vaccine are warranted.

  3. Geomorphologic mapping in the Ny Ålesund area (Svalbard Island, Norway) for the analysis of geomorphologic effects on rock slopes induced by glacier retreat in climate sensitive High Arctic regions

    NASA Astrophysics Data System (ADS)

    Miccadei, Enrico; Piacentini, Tommaso; Casacchia, Ruggero; Sparapani, Roberto

    2014-05-01

    The geomorphological effects of glacial retreat, rapidly changing Arctic environments and consequent local temporary permafrost melting are several types of glacial and periglacial landforms (pingos, solifluction, drumlins, etc.) but also debris and rock falls, alluvial fan and glacial outwash development and scarp/slopes retreat and evolution. In this work we have realized a geomorphologic map of rockfalls, landslides, alluvial fans and the slopes and scarps of steep mountainsides in the Ny Ålesund area (Svalbard Island, Norway) focused on the analysis of rock falls as geomorphological effects of glacier retreat, permafrost degradation and higher temperatures on slope processes. The investigation is based on geological and geomorphological field survey, and remote sensing and aerial photo interpretation, The Ny Ålesund area landscape is characterized by rugged non-vegetated mountains only partially covered by glaciers, with steep flanks and rock scarps; the scarps are formed by different types of rocks (intrusive and effusive igneous rocks, marine sedimentary rocks); this landscape is highly affected by debris and rock falls (from scarps and slopes) forming wide talus slopes and by alluvial fan and fluvial outwash (from glaciers), which make the surface sedimentary cover of the island together with rock glaciers and moraine deposits and locally fluvial deposits. The work is focused on the comprehension of the role of different factors in inducing rock falls, alluvial fans, slope/scarps evolution in high geomorphological sensitivity environments (i.e. glacial, periglacial or mountain) including: orography, lithology, rock fracturation, morphostructural setting, meteorological context. The conclusions focus on the possible geomorphological hazards affecting the Ny Ålesund area.

  4. The potential of a salt-tolerant plant (Distichlis spicata cv. NyPa Forage) to treat effluent from inland saline aquaculture and provide livestock feed on salt-affected farmland.

    PubMed

    Lymbery, Alan J; Kay, Gavin D; Doupé, Robert G; Partridge, Gavin J; Norman, Hayley C

    2013-02-15

    Dryland salinity is a major problem affecting food production from agricultural land in Australia and throughout the world. Although there is much interest in using saline groundwater to grow marine fish on salt-affected farmland, the disposal of nutrient enriched, saline aquaculture effluent is a major environmental problem. We investigated the potential of the salt-tolerant NyPa Forage plant (Distichlis spicata L. Greene var. yensen-4a) to trap nutrients from saline aquaculture effluent and subsequently to provide a fodder crop for livestock. Sub-surface flow wetlands containing NyPa Forage were constructed and their efficacy in removing total nitrogen, ammonia, nitrite/nitrate, total phosphorus and orthophosphate was monitored under different levels of nutrients and salinity. The wetlands removed 60-90% of total nitrogen loads and at least 85% of ammonia, nitrite/nitrate, total phosphorus and orthophosphate loads, with greater efficiency at high nutrient and low salinity levels. The above-ground yield, sodium, crude protein (CP) and in vitro dry matter digestibility (DMD) of NyPa Forage plants were measured after fertilisation with different nutrient levels and cropping at different frequencies. Yield of plants increased with increased nutrient, while nutritive value was greater when nutrients were applied but did not differ among nutrient levels. Yield was not affected by cropping frequency, but nutritive value was greatest when plants were cropped at intervals of 21 or 42 days. At optimum nutrient addition and cropping levels, the plants had a mean CP content of 16.7% and an in vitro DMD of 67.6%, equivalent to an energy value of 9.5 MJ kg(-1). Assuming an equivalent fibre content and voluntary food intake as grass hay, and no accumulation of other toxic minerals, these nutritive values would be sufficient for maintenance or moderate liveweight gains in dry adult sheep or cattle.

  5. Simultaneous cytoplasmic and nuclear protein expression of melanoma antigen-A family and NY-ESO-1 cancer-testis antigens represents an independent marker for poor survival in head and neck cancer.

    PubMed

    Laban, Simon; Atanackovic, Djordje; Luetkens, Tim; Knecht, Rainald; Busch, Chia-Jung; Freytag, Marcus; Spagnoli, Giulio; Ritter, Gerd; Hoffmann, Thomas K; Knuth, Alexander; Sauter, Guido; Wilczak, Waldemar; Blessmann, Marco; Borgmann, Kerstin; Muenscher, Adrian; Clauditz, Till S

    2014-09-01

    The prognosis of head and neck squamous cell carcinoma (HNSCC) patients remains poor. The identification of high-risk subgroups is needed for the development of custom-tailored therapies. The expression of cancer-testis antigens (CTAs) has been linked to a worse prognosis in other cancer types; however, their prognostic value in HNSCC is unclear because only few patients have been examined and data on CTA protein expression are sparse. A tissue microarray consisting of tumor samples from 453 HNSCC patients was evaluated for the expression of CTA proteins using immunohistochemistry. Frequency of expression and the subcellular expression pattern (nuclear, cytoplasmic, or both) was recorded. Protein expression of melanoma antigen (MAGE)-A family CTA, MAGE-C family CTA and NY-ESO-1 was found in approximately 30, 7 and 4% of tumors, respectively. The subcellular expression pattern in particular had a marked impact on the patients' prognosis. Median overall survival (OS) of patients with (i) simultaneous cytoplasmic and nuclear expression compared to (ii) either cytoplasmic or nuclear expression and (iii) negative patients was 23.0 versus 109.0 versus 102.5 months, for pan-MAGE (p < 0.0001), 46.6 versus 50.0 versus 109.0 for MAGE-A3/A4 (p = 0.0074) and 13.3 versus 50.0 versus 100.2 months for NY-ESO-1 (p = 0.0019). By multivariate analysis, these factors were confirmed as independent markers for poor survival. HNSCC patients showing protein expression of MAGE-A family members or NY-ESO-1 represent a subgroup with an extraordinarily poor survival. The development of immunotherapeutic strategies targeting these CTA may, therefore, be a promising approach to improve the outcome of HNSCC patients.

  6. MAGE-A1, MAGE-A3, and NY-ESO-1 can be upregulated on neuroblastoma cells to facilitate cytotoxic T lymphocyte-mediated tumor cell killing.

    PubMed

    Bao, Lei; Dunham, Kimberly; Lucas, Kenneth

    2011-09-01

    Approximately half of patients with stage IV neuroblastoma are expected to relapse despite current therapy, and when this occurs, there is little likelihood of achieving a cure. Very few clinical trials have been conducted to determine whether cellular immune responses could be harnessed to fight this tumor, largely because potential tumor antigens for cytotoxic T lymphocytes (CTL) are limited. MAGE-A1, MAGE-A3, and NY-ESO-1 are cancer-testis (CT) antigens expressed on a number of malignant solid tumors, including neuroblastoma, but many tumor cell lines down-regulate the expression of CT antigens as well as major histocompatibility (MHC) antigens, precluding recognition by antigen-specific T cells. If expression of cancer antigens on neuroblastoma could be enhanced pharmacologically, CT antigen-specific immunotherapy could be considered for this tumor. We have demonstrated that the expression of MAGE-A1, MAGE-A3, and NY-ESO-1 can be upregulated on neuroblastoma cells following exposure to pharmacologic levels of the demethylating agent 5-aza-2'-deoxycytidine (decitabine, DAC). Expression of NY-ESO-1, MAGE-A1, or MAGE-A3 was induced in 10/10 neuroblastoma cell lines after 5 days of exposure to DAC. Culture of neuroblastoma cell lines with IFN-γ was also associated with an increased expression of either MHC Class I or II by cytofluorometry, as reported by other groups. MAGE-A1, MAGE-A3, and NY-ESO-1-specific CTL were cultured from volunteer donors by stimulating peripheral blood mononuclear cells with dendritic cells pulsed with overlapping peptide mixes derived from full-length proteins, and these CTL preferentially lysed HLA partially matched, DAC-treated neuroblastoma and glioblastoma cell lines. These studies show that demethylating chemotherapy can be combined with IFN-γ to increase the expression of CT antigens and MHC molecules on neuroblastoma cells, and pre-treatment with these agents makes tumor cell lines more susceptible to CTL-mediated killing. These

  7. Photosynthetic and growth response of sugar maple (Acer saccharum Marsh.) mature trees and seedlings to calcium, magnesium, and nitrogen additions in the Catskill Mountains, NY, USA

    USGS Publications Warehouse

    Momen, Bahram; Behling, Shawna J; Lawrence, Gregory B.; Sullivan, Joseph H

    2015-01-01

    Decline of sugar maple in North American forests has been attributed to changes in soil calcium (Ca) and nitrogen (N) by acidic precipitation. Although N is an essential and usually a limiting factor in forests, atmospheric N deposition may cause N-saturation leading to loss of soil Ca. Such changes can affect carbon gain and growth of sugar maple trees and seedlings. We applied a 22 factorial arrangement of N and dolomitic limestone containing Ca and Magnesium (Mg) to 12 forest plots in the Catskill Mountain region of NY, USA. To quantify the short-term effects, we measured photosynthetic-light responses of sugar maple mature trees and seedlings two or three times during two summers. We estimated maximum net photosynthesis (An-max) and its related light intensity (PAR at An-max), apparent quantum efficiency (Aqe), and light compensation point (LCP). To quantify the long-term effects, we measured basal area of living mature trees before and 4 and 8 years after treatment applications. Soil and foliar chemistry variables were also measured. Dolomitic limestone increased Ca, Mg, and pH in the soil Oe horizon. Mg was increased in the B horizon when comparing the plots receiving N with those receiving CaMg. In mature trees, foliar Ca and Mg concentrations were higher in the CaMg and N+CaMg plots than in the reference or N plots; foliar Ca concentration was higher in the N+CaMg plots compared with the CaMg plots, foliar Mg was higher in the CaMg plots than the N+CaMg plots; An-max was maximized due to N+CaMg treatment; Aqe decreased by N addition; and PAR at An-max increased by N or CaMg treatments alone, but the increase was maximized by their combination. No treatment effect was detected on basal areas of living mature trees four or eight years after treatment applications. In seedlings, An-max was increased by N+CaMg addition. The reference plots had an open herbaceous layer, but the plots receiving N had a dense monoculture of common woodfern in the

  8. Photosynthetic and Growth Response of Sugar Maple (Acer saccharum Marsh.) Mature Trees and Seedlings to Calcium, Magnesium, and Nitrogen Additions in the Catskill Mountains, NY, USA

    PubMed Central

    Momen, Bahram; Behling, Shawna J.; Lawrence, Greg B.; Sullivan, Joseph H.

    2015-01-01

    Decline of sugar maple in North American forests has been attributed to changes in soil calcium (Ca) and nitrogen (N) by acidic precipitation. Although N is an essential and usually a limiting factor in forests, atmospheric N deposition may cause N-saturation leading to loss of soil Ca. Such changes can affect carbon gain and growth of sugar maple trees and seedlings. We applied a 22 factorial arrangement of N and dolomitic limestone containing Ca and Magnesium (Mg) to 12 forest plots in the Catskill Mountain region of NY, USA. To quantify the short-term effects, we measured photosynthetic-light responses of sugar maple mature trees and seedlings two or three times during two summers. We estimated maximum net photosynthesis (An-max) and its related light intensity (PAR at An-max), apparent quantum efficiency (Aqe), and light compensation point (LCP). To quantify the long-term effects, we measured basal area of living mature trees before and 4 and 8 years after treatment applications. Soil and foliar chemistry variables were also measured. Dolomitic limestone increased Ca, Mg, and pH in the soil Oe horizon. Mg was increased in the B horizon when comparing the plots receiving N with those receiving CaMg. In mature trees, foliar Ca and Mg concentrations were higher in the CaMg and N+CaMg plots than in the reference or N plots; foliar Ca concentration was higher in the N+CaMg plots compared with the CaMg plots, foliar Mg was higher in the CaMg plots than the N+CaMg plots; An-max was maximized due to N+CaMg treatment; Aqe decreased by N addition; and PAR at An-max increased by N or CaMg treatments alone, but the increase was maximized by their combination. No treatment effect was detected on basal areas of living mature trees four or eight years after treatment applications. In seedlings, An-max was increased by N+CaMg addition. The reference plots had an open herbaceous layer, but the plots receiving N had a dense monoculture of common woodfern in the forest floor

  9. Photosynthetic and Growth Response of Sugar Maple (Acer saccharum Marsh.) Mature Trees and Seedlings to Calcium, Magnesium, and Nitrogen Additions in the Catskill Mountains, NY, USA.

    PubMed

    Momen, Bahram; Behling, Shawna J; Lawrence, Greg B; Sullivan, Joseph H

    2015-01-01

    Decline of sugar maple in North American forests has been attributed to changes in soil calcium (Ca) and nitrogen (N) by acidic precipitation. Although N is an essential and usually a limiting factor in forests, atmospheric N deposition may cause N-saturation leading to loss of soil Ca. Such changes can affect carbon gain and growth of sugar maple trees and seedlings. We applied a 22 factorial arrangement of N and dolomitic limestone containing Ca and Magnesium (Mg) to 12 forest plots in the Catskill Mountain region of NY, USA. To quantify the short-term effects, we measured photosynthetic-light responses of sugar maple mature trees and seedlings two or three times during two summers. We estimated maximum net photosynthesis (An-max) and its related light intensity (PAR at An-max), apparent quantum efficiency (Aqe), and light compensation point (LCP). To quantify the long-term effects, we measured basal area of living mature trees before and 4 and 8 years after treatment applications. Soil and foliar chemistry variables were also measured. Dolomitic limestone increased Ca, Mg, and pH in the soil Oe horizon. Mg was increased in the B horizon when comparing the plots receiving N with those receiving CaMg. In mature trees, foliar Ca and Mg concentrations were higher in the CaMg and N+CaMg plots than in the reference or N plots; foliar Ca concentration was higher in the N+CaMg plots compared with the CaMg plots, foliar Mg was higher in the CaMg plots than the N+CaMg plots; An-max was maximized due to N+CaMg treatment; Aqe decreased by N addition; and PAR at An-max increased by N or CaMg treatments alone, but the increase was maximized by their combination. No treatment effect was detected on basal areas of living mature trees four or eight years after treatment applications. In seedlings, An-max was increased by N+CaMg addition. The reference plots had an open herbaceous layer, but the plots receiving N had a dense monoculture of common woodfern in the forest floor

  10. Photosynthetic and Growth Response of Sugar Maple (Acer saccharum Marsh.) Mature Trees and Seedlings to Calcium, Magnesium, and Nitrogen Additions in the Catskill Mountains, NY, USA.

    PubMed

    Momen, Bahram; Behling, Shawna J; Lawrence, Greg B; Sullivan, Joseph H

    2015-01-01

    Decline of sugar maple in North American forests has been attributed to changes in soil calcium (Ca) and nitrogen (N) by acidic precipitation. Although N is an essential and usually a limiting factor in forests, atmospheric N deposition may cause N-saturation leading to loss of soil Ca. Such changes can affect carbon gain and growth of sugar maple trees and seedlings. We applied a 22 factorial arrangement of N and dolomitic limestone containing Ca and Magnesium (Mg) to 12 forest plots in the Catskill Mountain region of NY, USA. To quantify the short-term effects, we measured photosynthetic-light responses of sugar maple mature trees and seedlings two or three times during two summers. We estimated maximum net photosynthesis (An-max) and its related light intensity (PAR at An-max), apparent quantum efficiency (Aqe), and light compensation point (LCP). To quantify the long-term effects, we measured basal area of living mature trees before and 4 and 8 years after treatment applications. Soil and foliar chemistry variables were also measured. Dolomitic limestone increased Ca, Mg, and pH in the soil Oe horizon. Mg was increased in the B horizon when comparing the plots receiving N with those receiving CaMg. In mature trees, foliar Ca and Mg concentrations were higher in the CaMg and N+CaMg plots than in the reference or N plots; foliar Ca concentration was higher in the N+CaMg plots compared with the CaMg plots, foliar Mg was higher in the CaMg plots than the N+CaMg plots; An-max was maximized due to N+CaMg treatment; Aqe decreased by N addition; and PAR at An-max increased by N or CaMg treatments alone, but the increase was maximized by their combination. No treatment effect was detected on basal areas of living mature trees four or eight years after treatment applications. In seedlings, An-max was increased by N+CaMg addition. The reference plots had an open herbaceous layer, but the plots receiving N had a dense monoculture of common woodfern in the forest floor

  11. Atmospheric black carbon concentrations in North America, ~1100 to 2005. Liaquat Husain and Tanveer Ahmed Wadsworth Center, New York State Department of Health, Albany, NY 12201-0509, USA, and Department of Environmental Health Sciences, School of Public Health, State University of New York, Albany, NY, 12201-0509, USA

    NASA Astrophysics Data System (ADS)

    Husain, L.

    2013-05-01

    Black carbon (BC) aerosols in the atmosphere absorb solar radiation, and cause heating of the atmosphere, may alter Earth's cloud cover, and impact precipitation cycle. The best estimate for radiative forcing from BC with 90% certainty is + 1.1 Wm-2 ( + 0.17 to 2.1 Wm-2), second only to that of CO2. A major uncertainty in the estimation is a lack of atmospheric BC data. Models are used to estimate BC emissions into the atmosphere. They have not been validated by field data. We report measurements of BC in the atmosphere, over a period of several centuries using lake sediments. Retrieval of such records provides an invaluable source for understanding changes in the atmosphere with time. Numerous studies have been conducted with such an objective. Owing to a lack of knowledge of deposition rates of atmospheric aerosols into the lake sediments, a major shortcoming of these studies have been an inability to convert the measurements of chemical species in lake sediments into the atmosphere. We have developed a technique to overcome this shortcoming by measuring black carbon in atmospheric aerosols, and in lake sediment cores. The concentrations of BC were determined in daily filters collected at Whiteface Mountain, NY, from 1978 to 2005. Cores from two lakes around Whiteface Mountain, NY, were collected. Cores were (1) sectioned in thin slices, (2) freeze dried, (3) dated using the 210Pb technique, (4) BC chemically separated, and (5) concentrations measured using the thermal-optical method. By The deposition rate of BC from the atmosphere to the lakes was determined by comparing the BC concentration in air and the sediments for the 1978 -2005 period. The deposition rate so determined was used to concert the BC in the sediment into the atmosphere for the ~1978 to ~1100 period. The BC concentrations for the industrial period, ~1850 was low but rapidly increased from ~1900, peaked ~1925, decreased very slowly ~ 1980, followed by a sharp decrease. Concentrations has

  12. Vaccination with a recombinant protein encoding the tumor-specific antigen NY-ESO-1 elicits an A2/157-165-specific CTL repertoire structurally distinct and of reduced tumor reactivity than that elicited by spontaneous immune responses to NY-ESO-1-expressing Tumors.

    PubMed

    Bioley, Gilles; Guillaume, Philippe; Luescher, Immanuel; Bhardwaj, Nina; Mears, Gregory; Old, Lloyd; Valmori, Danila; Ayyoub, Maha

    2009-01-01

    In a recent vaccination trial assessing the immunogenicity of an NY-ESO-1 (ESO) recombinant protein administered with Montanide and CpG, we have obtained evidence that this vaccine induces specific cytolytic T lymphocytes (CTL) in half of the patients. Most vaccine-induced CTLs were directed against epitopes located in the central part of the protein, between amino acids 81 and 110. This immunodominant region, however, is distinct from another ESO CTL region, 157-165, that is a frequent target of spontaneous CTL responses in A2+ patients bearing ESO tumors. In this study, we have investigated the CTL responses to ESO 157-165 in A2+ patients vaccinated with the recombinant protein. Our data indicate that after vaccination with the protein, CTL responses to ESO 157-165 are induced in some, but not all, A2+ patients. ESO 157-165-specific CTLs induced by vaccination with the ESO protein were functionally heterogeneous in terms of tumor recognition and often displayed decreased tumor reactivity as compared with ESO 157-165-specific CTLs isolated from patients with spontaneous immune responses to ESO. Remarkably, protein-induced CTLs used T-cell receptors similar to those previously isolated from patients vaccinated with synthetic ESO peptides (Vbeta4.1) and distinct from those used by highly tumor-reactive CTLs isolated from patients with spontaneous immune responses (Vbeta1.1, Vbeta8.1, and Vbeta13.1). Together, these results demonstrate that vaccination with the ESO protein elicits a repertoire of ESO 157-165-specific CTLs bearing T-cell receptors that are structurally distinct from those of CTLs found in spontaneous immune responses to the antigen and that are heterogeneous in terms of tumor reactivity, being often poorly tumor reactive.

  13. Growth of Ga0.46In0.54NyAs1-y Single Quantum Wells on InP(100) Substrate by Metalorganic Chemical Vapor Deposition

    NASA Astrophysics Data System (ADS)

    Ubukata, Akinori; Dong, Jie; Matsumoto, Koh; Ishihara, Yoshio

    2000-10-01

    GaInNAs has been demonstrated as a 1 eV material that is lattice-matched to GaAs. Similarly, it is expected that for GaInNAs, which is lattice-matched to InP with an In content of over 50%, a band gap from 0.7 to 0.3 eV should be achievable if a few percent N could be incorporated. A Ga0.46In0.54NyAs1-y/InP single quantum well (SQW) structure grown at a relatively high growth temperature has been attempted. Low AsH3 partial pressure appeared to enhance N incorporation. A strong photoluminescence (PL) emission was observed without post-growth annealing at the growth temperature of 650 to 680°C. For our reactor, the PL properties of GaInNAs appeared to improve for the growth pressure of 120 Torr since a narrow PL linewidth as low as 32 meV was obtained at that pressure. In the low-temperature PL measurement, blue shift was observed.

  14. NY-ESO-1-specific circulating CD4+ T cells in ovarian cancer patients are prevalently T(H)1 type cells undetectable in the CD25+ FOXP3+ Treg compartment.

    PubMed

    Redjimi, Nassima; Duperrier-Amouriaux, Karine; Raimbaud, Isabelle; Luescher, Immanuel; Dojcinovic, Danijel; Classe, Jean-Marc; Berton-Rigaud, Dominique; Frenel, Jean-Sébastien; Bourbouloux, Emmanuelle; Valmori, Danila; Ayyoub, Maha

    2011-01-01

    Spontaneous CD4(+) T-cell responses to the tumor-specific antigen NY-ESO-1 (ESO) are frequently found in patients with epithelial ovarian cancer (EOC). If these responses are of effector or/and Treg type, however, has remained unclear. Here, we have used functional approaches together with recently developed MHC class II/ESO tetramers to assess the frequency, phenotype and function of ESO-specific cells in circulating lymphocytes from EOC patients. We found that circulating ESO-specific CD4(+) T cells in EOC patients with spontaneous immune responses to the antigen are prevalently T(H)1 type cells secreting IFN-γ but no IL-17 or IL-10 and are not suppressive. We detected tetramer(+) cells ex vivo, at an average frequency of 1:25,000 memory cells, that is, significantly lower than in patients immunized with an ESO vaccine. ESO tetramer(+) cells were mostly effector memory cells at advanced stages of differentiation and were not detected in circulating CD25(+)FOXP3(+)Treg. Thus, spontaneous CD4(+) T-cell responses to ESO in cancer patients are prevalently of T(H)1 type and not Treg. Their relatively low frequency and advanced differentiation stage, however, may limit their efficacy, that may be boosted by immunogenic ESO vaccines.

  15. Pressure effect on the magnetism of the diluted magnetic semiconductor (B a1 -xKx ) (Zn1-yM ny ) 2A s2 with independent spin and charge doping

    NASA Astrophysics Data System (ADS)

    Sun, F.; Li, N. N.; Chen, B. J.; Jia, Y. T.; Zhang, L. J.; Li, W. M.; Zhao, G. Q.; Xing, L. Y.; Fabbris, G.; Wang, Y. G.; Deng, Z.; Uemura, Y. J.; Mao, H. K.; Haskel, D.; Yang, W. G.; Jin, C. Q.

    2016-06-01

    We used x-ray magnetic circular dichroism (XMCD) to probe the ferromagnetic properties of As p -symmetric (4 p ) states in the recently synthesized diluted magnetic semiconductor (B a1 -xKx) (Zn1-yM ny) 2A s2 system under ambient- and high-pressure conditions. The As K -edge XMCD signal scales with the sample magnetization (dominated by Mn) and scales with the ferromagnetic ordering temperature T c , and hence it is representative of the bulk magnetization. The XMCD intensity gradually decreases upon compression and vanishes at around 25 GPa, indicating quenching of ferromagnetism at this pressure. Transport measurements show a concomitant increase in conductivity with pressure, leading to a nearly metallic state at about the same pressure where magnetic order collapses. High-pressure x-ray diffraction shows an absence of structural transitions to 40 GPa. The results indicate that the mobility of doped holes, probed by both transport and x-ray absorption spectroscopy (4 p band broadening), is intimately connected with the mechanism of magnetic ordering in this class of compounds and that its control using external pressure provides an alternative route for tuning the magnetic properties in diluted magnetic semiconductor materials.

  16. Vertical distribution of non-volatile species of upper tropospheric and lower stratospheric aerosol observed by balloon-borne optical particle counter above Ny-Aalesund, Norway in the winter of 2015

    NASA Astrophysics Data System (ADS)

    Shiraishi, K.; Hayashi, M.; Shibata, T.; Neuber, R.; Ruhe, W.

    2015-12-01

    The polar lower stratosphere is the sink area of stratospheric global circulation. The composition, concentration and size distribution of aerosol in the polar stratosphere are considered to be strongly influenced by the transportations from mid-latitude to polar region and exchange of stratosphere to troposphere. In order to study the aerosol composition and size distribution in the Arctic stratosphere and the relationship between their aerosol microphysical properties and transport process, we carried out balloon-borne measurement of aerosol volatility above Ny-Aalesund, Norway in the winter of 2015. In our observation, two optical particle counters and a thermo denuder were suspended by one rubber balloon. A particle counter measured the heated aerosol size distribution (after heating at the temperature of 300 degree by the thermo denuder) and the other measured the ambient aerosol size distribution during the observation. The observation was carried out on 15 January, 2015. Balloon arrived at the height of 30km and detailed information of aerosol size distributions in upper troposphere and lower stratosphere for both heated aerosol and ambient aerosol were obtained. As a Result, the number ratio of non-volatile particles to ambient aerosol particles in lower stratosphere (11-15km) showed different feature in particle size range of fine mode (0.3

  17. Structural and chemical ordering of Heusler C oxM nyG ez epitaxial films on Ge (111): Quantitative study using traditional and anomalous x-ray diffraction techniques

    NASA Astrophysics Data System (ADS)

    Collins, B. A.; Chu, Y. S.; He, L.; Haskel, D.; Tsui, F.

    2015-12-01

    Epitaxial films of C oxM nyG ez grown on Ge (111) substrates by molecular-beam-epitaxy techniques have been investigated as a continuous function of composition using combinatorial synchrotron x-ray diffraction (XRD) and x-ray fluorescence (XRF) spectroscopy techniques. A high-resolution ternary epitaxial phase diagram is obtained, revealing a small number of structural phases stabilized over large compositional regions. Ordering of the constituent elements in the compositional region near the full Heusler alloy C o2MnGe has been examined in detail using both traditional XRD and a new multiple-edge anomalous diffraction (MEAD) technique. Multiple-edge anomalous diffraction involves analyzing the energy dependence of multiple reflections across each constituent absorption edge in order to detect and quantify the elemental distribution of occupation in specific lattice sites. Results of this paper show that structural and chemical ordering are very sensitive to the Co : Mn atomic ratio, such that the ordering is the highest at an atomic ratio of 2 but significantly reduced even a few percent off this ratio. The in-plane lattice is nearly coherent with that of the Ge substrate, while the approximately 2% lattice mismatch is accommodated by the out-of-plane tetragonal strain. The quantitative MEAD analysis further reveals no detectable amount (<0.5%) of Co-Mn site swapping, but instead high levels (26%) of Mn-Ge site swapping. Increasing Ge concentration above the Heusler stoichiometry (C o0.5M n0.25G e0.25) is shown to correlate with increased lattice vacancies, antisites, and stacking faults, but reduced lattice relaxation. The highest degree of chemical ordering is observed off the Heusler stoichiometry with a Ge enrichment of 5 at.%.

  18. CaCl[sub 2]-NaCl-H[sub 2]O fluid inclusions in the Box Vein of Lyonsdale,NY a fossil shield brine in northern, New York

    SciTech Connect

    Garside, A.R.; Darling, R.S. . Dept. of Geology)

    1993-03-01

    The Box Vein of Lyonsdale, NY is characterized by calcite-hosted, box-like cavities (equidimensional to tabular in shape) lined with comb-structured quartz. Quartz deposition was followed by late calcite, rare chalcopyrite, and solid organic matter (similar to anthraxolite associated with Herkimer quartz). The geologic setting of the vein is similar to Rossie-type veins in that it is hosted by Precambrian gneisses, but postdates Grenville metamorphism. Primary fluid inclusions in quartz (defined by growth zones) contain H[sub 2]O (vapor) + H[sub 2]O (brine) at room temperature. Upon warming from liquid nitrogen temperatures ([minus]196C), the ice and salt-hydrates gradually darkened and became granular between [minus]65C and [minus]52C, temperatures and phase behavior consistent with first melting in the CaCl[sub 2]-NaCl-H[sub 2]O system. Final ice melting temperatures average [minus]25.2C [plus minus] 1.4C (1 [sigma], N = 9). Final hydrohalite melting temperatures average [minus]13.2C [plus minus] 2.9C (1 [sigma], N = 6). Hydrohalite was distinguished from ice by its higher birefringence and lower relief. Inclusions homogenized to liquid at temperatures averaging 151C [plus minus] 7C (1 [sigma], N = 17). The microthermometric measurements were recorded by temperature cycling. Final melting temperatures of ice and hydrohalite indicate NaCl and CaCl[sub 2] contents of 17 [plus minus] 0.5 and 9 [plus minus] 1 wt. %, respectively (as modeled in the ternary CaCl[sub 2]-NaCl-H[sub 2]O system). The geologic setting, saline fluid chemistry, and relatively low temperature ([approximately]150C) of the Box Vein are similar to characteristics of shield brines described by Frape and Fritz in Canadian basement rocks.

  19. Sputtering characteristics, crystal structures, and transparent conductive properties of TiOxNy films deposited on α-Al2O3(0 0 0 1) and glass substrates

    NASA Astrophysics Data System (ADS)

    Akazawa, Housei

    2012-12-01

    Adding N2 gas during reactive sputtering of a Ti target prevented the target surface from being severely poisoned by oxygen atoms and sustained a high deposition rate for titanium oxynitride films under metal-mode-like sputtering conditions. With progress in the degree of oxidization, films deposited onto a glass substrate varied from TiO1-xNx having a face-centered cubic (fcc) structure to TiO2-xNx having an anatase structure. Titanium oxynitride films deposited on an Al2O3(0 0 0 1) substrate were epitaxial with major orientations toward the (1 1 1) and (2 0 0) directions for fcc-TiO1-xNx and (1 1 2) for anatase-TiO2-xNx. Intermediately oxidized films between TiO1-xNx and TiO2-xNx were amorphous on the glass substrate but crystallized into a Magneli phase, TinO(N)2n-1, on the Al2O3(0 0 0 1) substrate. Partially substituting oxygen in TiO2 with nitrogen as well as continuously irradiating the growing film surface with a Xe plasma stream preferentially formed anatase rather than rutile. However, the occupation of anion sites with enough oxygen rather than nitrogen was the required condition for anatase crystals to form. The transparent conductive properties of epitaxial TiO2-xNx films on Al2O3(0 0 0 1) were superior to those of microcrystalline films on the glass substrate. Since resistivity and optical transmittance of TiOxNy films vary continuously with changing N2 flow rate, their transparent conductive properties can be controlled more easily than TiOx. Nb5+ ions could be doped as donors in TiO2-xNx anatase crystals.

  20. Zeroing in on Tumor-Reactive TILs.

    PubMed

    Ohashi, Pamela S

    2016-09-01

    Adoptive cell transfer of tumor-specific T cells provides an effective strategy for cancer immunotherapy. An article in this issue provides a novel approach to refine this technology by identifying tumor-reactive T cells based on frequency and PD-1 expression. Cancer Immunol Res; 4(9); 719. ©2016 AACRSee article by Ascierto et al., p. 726. PMID:27590279

  1. William Van Til: The Last Progressive?

    ERIC Educational Resources Information Center

    Beineke, John A.

    2010-01-01

    In 1999, the changing goals of American schools were explored in "Education Week" through the events, achievements, and personalities that had formed United States education in the 20th century. First a series of articles, the collection was later published in book form as "Lessons of a Century: A Nation's Schools Come of Age." The chapter titled…

  2. 49 CFR 372.227 - Syracuse, NY.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... are within an area bounded by a line beginning at the intersection of new New York Highway 48 with the line described in (b) of this sectio, thence northwesterly along new New York Highway 48 to junction New York Highway 370, thence westerly along New York Highway 370 to junction Emerick Road,...

  3. 49 CFR 372.227 - Syracuse, NY.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... are within an area bounded by a line beginning at the intersection of new New York Highway 48 with the line described in (b) of this sectio, thence northwesterly along new New York Highway 48 to junction New York Highway 370, thence westerly along New York Highway 370 to junction Emerick Road,...

  4. 49 CFR 372.227 - Syracuse, NY.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... are within an area bounded by a line beginning at the intersection of new New York Highway 48 with the line described in (b) of this sectio, thence northwesterly along new New York Highway 48 to junction New York Highway 370, thence westerly along New York Highway 370 to junction Emerick Road,...

  5. 49 CFR 372.227 - Syracuse, NY.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... are within an area bounded by a line beginning at the intersection of new New York Highway 48 with the line described in (b) of this sectio, thence northwesterly along new New York Highway 48 to junction New York Highway 370, thence westerly along New York Highway 370 to junction Emerick Road,...

  6. NY State Profile. New York: Regents Examinations

    ERIC Educational Resources Information Center

    Center on Education Policy, 2010

    2010-01-01

    This paper provides information about the New York's Regents Examination. The purpose of the exam is to: (1) Provide schools with student academic diagnostic information; (2) Determine prospective high school graduates' knowledge and skill levels relative to those needed for entry-level employment; (3) Determine prospective high school graduates'…

  7. Acceleration Of Wound Healing Ny Photodynamic Therapy

    DOEpatents

    Hasan, Tayyaba; Hamblin, Michael R.; Trauner, Kenneth

    2000-08-22

    Disclosed is a method for accelerating wound healing in a mammal. The method includes identifying an unhealed wound site or partially-healed wound site in a mammal; administering a photosensitizer to the mammal; waiting for a time period wherein the photosensitizer reaches an effective tissue concentration at the wound site; and photoactivating the photosensitizer at the wound site. The dose of photodynamic therapy is selected to stimulate the production of one or more growth factor by cells at the wound site, without causing tissue destruction.

  8. Solar Market Analytics, Roadmapping, and Tracking NY (SMART NY) Final Report

    SciTech Connect

    Case, Tria; Reilly, Laurie; Kling, Alison

    2014-05-15

    This is the final report, including links to Working Group reports and an attached Working Group report for SunShot Initiative Rooftop Solar Challenge I, from Sustainable CUNY of the City University of New York, on behalf of New York City

  9. Expansion of Tumor-reactive T Cells From Patients With Pancreatic Cancer.

    PubMed

    Meng, Qingda; Liu, Zhenjiang; Rangelova, Elena; Poiret, Thomas; Ambati, Aditya; Rane, Lalit; Xie, Shanshan; Verbeke, Caroline; Dodoo, Ernest; Del Chiaro, Marco; Löhr, Matthias; Segersvärd, Ralf; Maeurer, Markus J

    2016-01-01

    Generation of T lymphocytes with reactivity against cancer is a prerequisite for effective adoptive cellular therapies. We established a protocol for tumor-infiltrating lymphocytes (TILs) from patients with pancreatic ductal adenocarcinoma. Tumor samples from 17 pancreatic cancer specimens were cultured with cytokines (IL-2, IL-15, and IL-21) to expand TILs. After 10 days of culture, TILs were stimulated with an anti-CD3 antibody (OKT3) and irradiated allogeneic peripheral blood mononuclear cells. Reactivity of TILs against tumor-associated antigens (mesothelin, survivin, or NY-ESO-1) was detected by intracellular cytokine production by flow cytometry. Cytotoxicity was measured using a Chromium 51 release assay, and reactivity of TILs against autologous tumor cells was detected by INF-[gamma] production (ELISA). TIL composition was tested by CD45RA, CCR7, 4-1BB, LAG-3, PD-1, TIM3, and CTLA-4 marker analysis. TCR V[beta] was determined by flow cytometry and TCR clonality was gauged measuring the CDR3 region length by PCR analysis and subsequent sequencing. We could reliably obtain TILs from 17/17 patients with a majority of CD8(+) T cells. CD3(+)CD8(+), CD3(+)CD4(+), and CD3(+)CD4(-)CD8(-)[double-negative (DN) T cells] resided predominantly in central (CD45RA(-)CCR7(+)) and effector (CD45RA-CCR7-) memory subsets. CD8(+) TILs tested uniformly positive for LAG-3 (about 100%), whereas CD4(+) TILs showed only up to 12% LAG-3(+) staining and PD-1 showed a broad expression pattern in TILs from different patients. TILs from individual patients recognized strongly (up to 11.9% and 8.2% in CD8(+)) NY-ESO-1, determined by ICS, or mesothelin, determined respectively by TNF-[alpha] and IFN-[gamma] production. Twelve of 17 of CD8(+) TILs showed preferential expansion of certain TCR V[beta] families (eg, 99.2% V[beta]13.2 in CD8(+) TILs, 77% in the V[beta]1, 65.9% in the V[beta]22, and 63.3% in the V[beta]14 family). TCR CDR3 analysis exhibited monoclonal or oligoclonal TCRs

  10. Study of marble deterioration at City Hall, Schenectady, NY

    SciTech Connect

    Cheng, R.J.; Castillo, R.

    1984-01-01

    The effects of air pollution and acid precipitation were investigated as causing the erosion of marble. The research indicated that the marble grains are being structurally weakened by a chemical conversion process of marble to gypsum crystals. The surface zone of chemical activity (2-5 mm) shows the presence of fly ash and iron particles, and points to the possibility of a catalytic mechanism for the conversion of sulfur dioxide to sulfate.

  11. 77 FR 22530 - Safety Zone; Fireworks, Hudson River, Rhinecliff, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-16

    ... Federal Register (73 FR 3316). Public Meeting We do not plan now to hold a public meeting. But you may... safety zone. Before the activation of the zone, the Coast Guard will issue maritime advisories widely... distribution of power and responsibilities between the Federal Government and Indian tribes. Energy Effects...

  12. 78 FR 27031 - Amendment of Class E Airspace; Kingston, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... (78 FR 6260) Docket No. FAA-2012-0831. Interested parties were invited to participate in this... Executive Order 12866; (2) is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR...: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p....

  13. 76 FR 56856 - New York Disaster Number NY-00108

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-14

    ... York (FEMA-4020-DR), dated 08/31/ 2011. Incident: Hurricane Irene. Incident Period: 08/26/2011 and continuing. Effective Date: 09/01/2011. Physical Loan Application Deadline Date: 10/31/2011. EIDL Loan Application Deadline Date: 05/31/2012. ADDRESSES: Submit completed loan applications to: U.S. Small...

  14. 78 FR 72023 - Drawbridge Operation Regulation; Genessee River, Rochester, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-02

    ... longer exists. This change does not affect waterway or land traffic. This change does not affect nor does... affect the way vessels operate on the waterway. 2. Impact on Small Entities The Regulatory Flexibility... impact of regulations on small entities during rulemaking. The term ``small entities'' comprises...

  15. Mill Seat Landfill Bioreactor Renewable Green Power (NY)

    SciTech Connect

    Barton & Loguidice, P.C.

    2010-01-07

    The project was implemented at the Mill Seat landfill located in the Town of Bergen, Monroe County, New York. The landfill was previously equipped with a landfill gas collection system to collect methane gas produced by the bioreactor landfill and transport it to a central location for end use. A landfill gas to energy facility was also previously constructed at the site, which utilized generator engines, designed to be powered with landfill methane gas, to produce electricity, to be utilized on site and to be sold to the utility grid. The landfill gas generation rate at the site had exceeded the capacity of the existing generators, and the excess landfill gas was therefore being burned at a candlestick flare for destruction. The funded project consisted of the procurement and installation of two (2) additional 800 KW Caterpillar 3516 generator engines, generator sets, switchgear and ancillary equipment.

  16. Engineering economic assessment of residential wood heating in NY

    EPA Science Inventory

    We provide insight into the recent resurgence in residential wood heating in New York by: (i) examining the lifetime costs of outdoor wood hydronic heaters (OWHHs) and other whole-house residential wood heat devices,(ii) comparing these lifetime costs with those of competing tech...

  17. Rust City: Brockport College Apartments, Brockport, N.Y.

    ERIC Educational Resources Information Center

    Stephens, Suzanne

    1975-01-01

    To hold down construction costs of student housing, Brockport College decided on a fixed-cost competition, in which architects had to team up with contractors. The winning firm used a French industrialized building system and exterior panels of weathered steel to construct a village of 27 four-story buildings. (JG)

  18. 77 FR 14690 - Drawbridge Operation Regulations; Hutchinson River, Bronx, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-13

    ... the Amtrak Pelham Bay Railroad Bridge, mile 0.5, across the Hutchinson River at the Bronx, New York... Transportation, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590... Bronx, New York, has a vertical clearance in the closed position of 8 feet at mean high water and...

  19. 75 FR 21371 - New York Disaster # NY-00089

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-23

    ... State of New York (FEMA- 1899-DR), dated 04/16/2010. Incident: Severe Storms and Flooding. Incident... disaster declaration on 04/16/2010, Private Non- Profit organizations that provide essential services of... Physical Damage: Non-Profit Organizations With Credit Available Elsewhere... 3.625 Non-Profit...

  20. Late Holocene Environmental Changes from NY-NJ Estuaries

    NASA Technical Reports Server (NTRS)

    Peteet, Dorothy M.

    2000-01-01

    High-resolution records of environmental change in the lower Hudson estuary are quite rare. We present preliminary data from several marshes in the New York- New Jersey region in order to understand the late Holocene environmental history of this region. Our project includes salt marsh cores from Hackensack, Piermont, Staten Island, and Jamaica Bay. Our preliminary research has focused on a 11.15 m sediment core from Piermont Marsh, New York (40 deg N, 74 deg W) in an attempt to document the Holocene environmental history of the region. Lithology, loss -on -ignition (LOI), pollen, plant macrofossils, charcoal, and foraminifera were analyzed. Core lithology consists of peat, silts, and clays that vary in color and texture. The base of the core is AMS C-14 dated to 4190 yr BP. Preliminary low-resolution analysis of the core to date includes sampling at the 1-meter interval throughout the core. LOI of the sediments ranges from 1% to 85%. Average rate of deposition is about .26 cm/yr. Major changes in pollen percentages are visible throughout the core.