Science.gov

Sample records for antigen retrieval methods

  1. How does antigen retrieval work?

    PubMed

    Leong, Trishe Y-M; Leong, Anthony S-Y

    2007-03-01

    The introduction of antigen retrieval has enabled immunohistology to become an integral component of morphologic diagnosis, routinely employed in cancer diagnosis, and for the identification of therapeutic and prognostic markers. The mechanism of antigen retrieval, however, remains speculative with the key to our understanding embedded in the actions of formaldehyde on proteins. One commonly held concept is that heat primarily breaks down protein cross-linkages that occur with aldehyde fixation, thus "unmasking" protein epitopes of interest. Enzymatic pretreatment is also thought to have a similar action whereas such "breakages" are the result of extremely rapid molecular movement induced by microwaves and ultrasound. The formation of rigid cagelike calcium complexes during formaldehyde fixation is another suggested mechanism of antigen masking requiring chelating agents for reversal. A more recent suggestion for the antigen retrieval phenomenon has evoked the Mannich reaction, which occurs with the cross-linking of some proteins. Such cross-linkages can be hydrolyzed by heat or alkalis so that the process of antigen retrieval may be the simple removal of such cross-linked proteins that are sterically interfering with the binding of antibodies to linear protein epitopes in the tissue section. We are clearly not yet in possession of all the answers to the problem.

  2. A New Antigen Retrieval Technique for Human Brain Tissue

    PubMed Central

    Byne, William; Haroutunian, Vahram; García-Villanueva, Mercedes; Rábano, Alberto; García-Amado, María; Prensa, Lucía; Giménez-Amaya, José Manuel

    2008-01-01

    Immunohistochemical staining of tissues is a powerful tool used to delineate the presence or absence of an antigen. During the last 30 years, antigen visualization in human brain tissue has been significantly limited by the masking effect of fixatives. In the present study, we have used a new method for antigen retrieval in formalin-fixed human brain tissue and examined the effectiveness of this protocol to reveal masked antigens in tissues with both short and long formalin fixation times. This new method, which is based on the use of citraconic acid, has not been previously utilized in brain tissue although it has been employed in various other tissues such as tonsil, ovary, skin, lymph node, stomach, breast, colon, lung and thymus. Thus, we reported here a novel method to carry out immunohistochemical studies in free-floating human brain sections. Since fixation of brain tissue specimens in formaldehyde is a commonly method used in brain banks, this new antigen retrieval method could facilitate immunohistochemical studies of brains with prolonged formalin fixation times. PMID:18852880

  3. Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: myosin-V assay.

    PubMed

    Calábria, Luciana Karen; Teixeira, Renata Roland; Coelho Gonçalves, Sybelli Magda; Passos Lima, Andreia Barcelos; Santos, Ana Alice Diniz Dos; Martins, Antônio Roberto; Espindola, Foued Salmen

    2010-01-01

    The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain.

  4. Instability of induction cooker (electromagnetic stove) antigen retrieval in immunohistochemistry.

    PubMed

    Ding, Wei; Zheng, Xiang-Yi

    2012-03-01

    An induction cooker is a modern electric cooker that takes electromagnetic induction principle to heat. As it has high efficiency, no open flame, and is safe and convenient, more and more laboratories use it as an antigen retrieval heating tool in immunohistochemistry. We found that there was still some instability with the induction cooker, because with certain antigens the power change influenced the results of immunohistochemistry staining, showing weaker staining intensity or decreased number of positive cells, but which were not entirely negative. For some antigens, it had no influence on results. The instability of this heating tool for antigen retrieval was caused partly by negligent operators, and which may influence the experimental results and the pathologic diagnosis.

  5. EFFECTIVENESS OF INFORMATION RETRIEVAL METHODS.

    ERIC Educational Resources Information Center

    SWETS, JOHN A.

    RESULTS OF FIFTY DIFFERENT RETRIEVAL METHODS AS APPLIED IN THREE EXPERIMENTAL RETRIEVAL SYSTEMS WERE SUBJECTED TO AN ANALYSIS SUGGESTED BY STATISTICAL DECISION THEORY. THE ANALYSIS USES A PREVIOUSLY-PROPOSED MEASURE OF EFFECTIVENESS AND DEMONSTRATES ITS SEVERAL PROPERTIES. SOME OF THESE PROPERTIES ARE--(1) IT ENABLES THE RETRIEVAL SYSTEM TO…

  6. Immunohistochemical detection of disease-associated prion protein in the intestine of cattle naturally affected with bovine spongiform encephalopathy by using an alkaline-based chemical antigen retrieval method.

    PubMed

    Okada, Hiroyuki; Iwamaru, Yoshihumi; Imamura, Morikazu; Masujin, Kentaro; Yokoyama, Takashi; Mohri, Shirou

    2010-11-01

    An alkaline-based chemical antigen retrieval pretreatment step was used to enhance immunolabeling of disease-associated prion protein (PrP(Sc)) in formalin-fixed and paraffin-embedded tissue sections from cattle naturally affected with bovine spongiform encephalopathy (BSE). The modified chemical method used in this study amplified the PrP(Sc) signal by unmasking PrP(Sc) compared with the normal cellular prion protein. In addition, this method reduced nonspecific background immunolabeling that resulted from the destruction of the residual normal cellular form of prion protein, and reduced the treatment time compared with the usual autoclave pretreatment step. Immunolabeled PrP(Sc) was thereby clearly detected in the myenteric plexus of the ileum in naturally occurring BSE cattle.

  7. Electrochemical antigen-retrieval of formaldehyde fixed and paraffin-embedded archived leprosy skin biopsies.

    PubMed

    Sergio, Navarro-Fierros; Cecilia, Guillen-Vargas; Sonia, Luquin; la Mora Pedro, Garzon-De; Joaquin, Garcia-Estrada; Mary, Fafutis-Morris

    2004-06-01

    While formaldehyde fixation preserves tissue morphology, it often hinders immunodetection of antigens in paraffin-embedded tissue because the antigens are masked. Antigen unmasking can be achieved with treatments such as microwave irradiation but they often lead to excessive tissue damage. Therefore, an electrochemical antigen-retrieval method (EAR) was devised in which an alternating electric current is passed through the tissue in a chamber containing an electrolyte buffer. The results obtained with this method were compared to those after microwave irradiation using archived samples of formaldehyde-fixed and paraffin-embedded lepromatous leprosy skin. The efficacy of the two unmasking procedures was assessed by the immunodetectability of several marker antigens using 24 antibodies. Fifteen antibodies that were directed against transmembrane proteins (CD), and the remaining 9 against cytokeratins 18.6 and 19, laminin, vimentin, S100a, BCG, Ulex europaeus lectin, PCNA, and P21ras. Simple and double immunohistochemistry was performed using the universal ENVISION and LSAB + AP detection systems. After unmasking with the EAR method, immunoreactivity was clearly detected with 22 of the 24 antibodies in single labeling reactions. They include the critical antigens CD3 and CD4 for identifying the T lymphocyte lineages. In contrast, only 20 of the antibodies reacted after microwave irradiation. After double immunolabeling, immunoreactivity was quantitatively similar with both methods. However, the EAR unmasking produced a stronger labeling reaction. Thus, with double labeling immunohistochemistry, EAR made it possible to use higher antibody dilutions and shorter incubation times. Heat damage was also prevented. In conclusion, EAR treatment produces better staining results than microwave irradiation treatment.

  8. Robust keyword retrieval method for OCRed text

    NASA Astrophysics Data System (ADS)

    Fujii, Yusaku; Takebe, Hiroaki; Tanaka, Hiroshi; Hotta, Yoshinobu

    2011-01-01

    Document management systems have become important because of the growing popularity of electronic filing of documents and scanning of books, magazines, manuals, etc., through a scanner or a digital camera, for storage or reading on a PC or an electronic book. Text information acquired by optical character recognition (OCR) is usually added to the electronic documents for document retrieval. Since texts generated by OCR generally include character recognition errors, robust retrieval methods have been introduced to overcome this problem. In this paper, we propose a retrieval method that is robust against both character segmentation and recognition errors. In the proposed method, the insertion of noise characters and dropping of characters in the keyword retrieval enables robustness against character segmentation errors, and character substitution in the keyword of the recognition candidate for each character in OCR or any other character enables robustness against character recognition errors. The recall rate of the proposed method was 15% higher than that of the conventional method. However, the precision rate was 64% lower.

  9. Evolutionary Computing Methods for Spectral Retrieval

    NASA Technical Reports Server (NTRS)

    Terrile, Richard; Fink, Wolfgang; Huntsberger, Terrance; Lee, Seugwon; Tisdale, Edwin; VonAllmen, Paul; Tinetti, Geivanna

    2009-01-01

    A methodology for processing spectral images to retrieve information on underlying physical, chemical, and/or biological phenomena is based on evolutionary and related computational methods implemented in software. In a typical case, the solution (the information that one seeks to retrieve) consists of parameters of a mathematical model that represents one or more of the phenomena of interest. The methodology was developed for the initial purpose of retrieving the desired information from spectral image data acquired by remote-sensing instruments aimed at planets (including the Earth). Examples of information desired in such applications include trace gas concentrations, temperature profiles, surface types, day/night fractions, cloud/aerosol fractions, seasons, and viewing angles. The methodology is also potentially useful for retrieving information on chemical and/or biological hazards in terrestrial settings. In this methodology, one utilizes an iterative process that minimizes a fitness function indicative of the degree of dissimilarity between observed and synthetic spectral and angular data. The evolutionary computing methods that lie at the heart of this process yield a population of solutions (sets of the desired parameters) within an accuracy represented by a fitness-function value specified by the user. The evolutionary computing methods (ECM) used in this methodology are Genetic Algorithms and Simulated Annealing, both of which are well-established optimization techniques and have also been described in previous NASA Tech Briefs articles. These are embedded in a conceptual framework, represented in the architecture of the implementing software, that enables automatic retrieval of spectral and angular data and analysis of the retrieved solutions for uniqueness.

  10. Comparative exoplanetology with consistent retrieval methods

    NASA Astrophysics Data System (ADS)

    Barstow, Joanna Katy; Aigrain, Suzanne; Irwin, Patrick Gerard Joseph; Sing, David

    2016-10-01

    The number of hot Jupiters with broad wavelength spectroscopic data has finally become large enough to make comparative planetology a reasonable proposition. New results presented by Sing et al. (2016) showcase ten hot Jupiters with spectra from the Hubble Space Telescope and photometry from Spitzer, providing insights into the presence of clouds and hazes.Spectral retrieval methods allow interpretation of exoplanet spectra using simple models, with minimal prior assumptions. This is particularly useful for exotic exoplanets, for which we may not yet fully understand the physical processes responsible for their atmospheric characteristics. Consistent spectral retrieval of a range of exoplanets can allow robust comparisons of their derived atmospheric properties.I will present a retrieval analysis using the NEMESIS code (Irwin et al. 2008) of the ten hot Jupiter spectra presented by Sing et al. (2016). The only distinctive aspects of the model for each planet are the mass and radius, and the temperature range explored. All other a priori model parameters are common to all ten objects. We test a range of cloud and haze scenarios, which include: Rayleigh-dominated and grey clouds; different cloud top pressures; and both vertically extended and vertically confined clouds.All ten planets, with the exception of WASP-39b, can be well represented by models with at least some haze or cloud. Our analysis of cloud properties has uncovered trends in cloud top pressure, vertical extent and particle size with planet equilibrium temperature. Taken together, we suggest that these trends indicate condensation and sedimentation of at least two different cloud species across planets of different temperatures, with condensates forming higher up in hotter atmospheres and moving progressively further down in cooler planets.Sing, D. et al. (2016), Nature, 529, 59Irwin, P. G. J. et al. (2008), JQSRT, 109, 1136

  11. p53 gene product expression in resected non-small cell carcinoma of the lung, with studies of concurrent cytological preparations and microwave antigen retrieval.

    PubMed Central

    Binks, S; Clelland, C A; Ronan, J; Bell, J

    1997-01-01

    AIM: To document the frequency and extent of p53 gene product expression in paraffin sections of resected non-small cell carcinoma of the lung and in cytological preparations of the same tumours; to determine the effect of microwave antigen retrieval on antigen detection. METHODS: Representative paraffin sections of 50 non-small cell carcinomas were stained with an antibody to p53 gene product (DO-7) both with and without prior microwave antigen retrieval. Cytoblocks and cell smears obtained from 19 cases were similarly stained. RESULTS: Using a histochemical scoring system (0-300) which takes into account staining intensity and extent, 78% (n = 39) of microwave pretreated paraffin sections and 52% (n = 26) of non-pretreated sections scored between 5 and 300; p = 0.001; 56% (n = 28) of microwave pretreated sections and only 2% (n = 1) of non-pretreated sections scored between 100 and 300 (p = 0.0001); 75% of direct smears of tumours and 80% of cytoblocks stained similarly to the paraffin sections of the resected specimens. No smears or cytoblocks stained positively when the sections of the resected specimen were negative. CONCLUSIONS: As up to 78% of non-small cell lung carcinomas overexpress p53 gene product, this may prove to be a valuable diagnostic method in biopsy or cytological material when the morphological diagnosis is uncertain. Microwave antigen retrieval is effective on formalin fixed tissue. Images PMID:9215149

  12. Advanced Feedback Methods in Information Retrieval.

    ERIC Educational Resources Information Center

    Salton, G.; And Others

    1985-01-01

    In this study, automatic feedback techniques are applied to Boolean query statements in online information retrieval to generate improved query statements based on information contained in previously retrieved documents. Feedback operations are carried out using conventional Boolean logic and extended logic. Experimental output is included to…

  13. Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining

    PubMed Central

    2016-01-01

    Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions. Minimizing the incubation time in fixative reduces disruption of tissue morphology, and reducing the incubation time in staining solution or antibody solution decreases nonspecific labeling. Reduction of incubation time in staining solution also decreases the level of background noise. Microwave-assisted tissue preparation is applicable for tissue fixation, decalcification of bone tissues, treatment of adipose tissues, antigen retrieval, and other special staining of tissues. Microwave-assisted tissue fixation and staining are useful tools for histological analyses. This review describes the protocols using microwave irradiation for several essential procedures in histochemical studies, and these techniques are applicable to other protocols for tissue fixation and immunostaining in the field of cell biology. PMID:27840640

  14. Using optimal estimation method for upper atmospheric Lidar temperature retrieval

    NASA Astrophysics Data System (ADS)

    Zou, Rongshi; Pan, Weilin; Qiao, Shuai

    2016-07-01

    Conventional ground based Rayleigh lidar temperature retrieval use integrate technique, which has limitations that necessitate abandoning temperatures retrieved at the greatest heights due to the assumption of a seeding value required to initialize the integration at the highest altitude. Here we suggests the use of a method that can incorporate information from various sources to improve the quality of the retrieval result. This approach inverts lidar equation via optimal estimation method(OEM) based on Bayesian theory together with Gaussian statistical model. It presents many advantages over the conventional ones: 1) the possibility of incorporating information from multiple heterogeneous sources; 2) provides diagnostic information about retrieval qualities; 3) ability of determining vertical resolution and maximum height to which the retrieval is mostly independent of the a priori profile. This paper compares one-hour temperature profiles retrieved using conventional and optimal estimation methods at Golmud, Qinghai province, China. Results show that OEM results show a better agreement with SABER profile compared with conventional one, in some region it is much lower than SABER profile, which is a very different results compared with previous studies, further studies are needed to explain this phenomenon. The success of applying OEM on temperature retrieval is a validation for using as retrieval framework in large synthetic observation systems including various active remote sensing instruments by incorporating all available measurement information into the model and analyze groups of measurements simultaneously to improve the results.

  15. Method for comparing content based image retrieval methods

    NASA Astrophysics Data System (ADS)

    Barnard, Kobus; Shirahatti, Nikhil V.

    2003-01-01

    We assume that the goal of content based image retrieval is to find images which are both semantically and visually relevant to users based on image descriptors. These descriptors are often provided by an example image--the query by example paradigm. In this work we develop a very simple method for evaluating such systems based on large collections of images with associated text. Examples of such collections include the Corel image collection, annotated museum collections, news photos with captions, and web images with associated text based on heuristic reasoning on the structure of typical web pages (such as used by Google(tm)). The advantage of using such data is that it is plentiful, and the method we propose can be automatically applied to hundreds of thousands of queries. However, it is critical that such a method be verified against human usage, and to do this we evaluate over 6000 query/result pairs. Our results strongly suggest that at least in the case of the Corel image collection, the automated measure is a good proxy for human evaluation. Importantly, our human evaluation data can be reused for the evaluation of any content based image retrieval system and/or the verification of additional proxy measures.

  16. Group-Based Image Retrieval Method for Video Image Annotation

    NASA Astrophysics Data System (ADS)

    Murabayashi, Noboru; Kurahashi, Setsuya; Yoshida, Kenichi

    This paper proposes a group-based image retrieval method for video image annotation systems. Although the wide spread use of video camera recorders has increased the demand for an automated annotation system for personal videos, conventional image retrieval methods cannot achieve enough accuracy to be used as an annotation engine. Recording conditions, such as change of the brightness by weather condition, shadow by the surroundings, and etc, affect the qualities of images recorded by the personal video camera recorders. The degraded image of personal video makes the retrieval task difficult. Furthermore, it is difficult to discriminate similar images without any auxiliary information. To cope with these difficulties, this paper proposes a group-based image retrieval method. Its characteristics are 1) the use of image similarity based on the wavelet transformation based features and the scale invariant feature transform based features, and 2) the pre-grouping of related images and screening using group information. Experimental results show that the proposed method can improve image retrieval accuracy to 90% up from the conventional method of 40%.

  17. Efficient Methods to Assimilate Satellite Retrievals Based on Information Content. Part 2; Suboptimal Retrieval Assimilation

    NASA Technical Reports Server (NTRS)

    Joiner, J.; Dee, D. P.

    1998-01-01

    One of the outstanding problems in data assimilation has been and continues to be how best to utilize satellite data while balancing the tradeoff between accuracy and computational cost. A number of weather prediction centers have recently achieved remarkable success in improving their forecast skill by changing the method by which satellite data are assimilated into the forecast model from the traditional approach of assimilating retrievals to the direct assimilation of radiances in a variational framework. The operational implementation of such a substantial change in methodology involves a great number of technical details, e.g., pertaining to quality control procedures, systematic error correction techniques, and tuning of the statistical parameters in the analysis algorithm. Although there are clear theoretical advantages to the direct radiance assimilation approach, it is not obvious at all to what extent the improvements that have been obtained so far can be attributed to the change in methodology, or to various technical aspects of the implementation. The issue is of interest because retrieval assimilation retains many practical and logistical advantages which may become even more significant in the near future when increasingly high-volume data sources become available. The central question we address here is: how much improvement can we expect from assimilating radiances rather than retrievals, all other things being equal? We compare the two approaches in a simplified one-dimensional theoretical framework, in which problems related to quality control and systematic error correction are conveniently absent. By assuming a perfect radiative transfer model and perfect knowledge of radiance and background error covariances, we are able to formulate a nonlinear local error analysis for each assimilation method. Direct radiance assimilation is optimal in this idealized context, while the traditional method of assimilating retrievals is suboptimal because it

  18. Retrieval practice can eliminate list method directed forgetting.

    PubMed

    Abel, Magdalena; Bäuml, Karl-Heinz T

    2016-01-01

    It has recently been shown that retrieval practice can reduce memories' susceptibility to interference, like retroactive and proactive interference. In this study, we therefore examined whether retrieval practice can also reduce list method directed forgetting, a form of intentional forgetting that presupposes interference. In each of two experiments, subjects successively studied two lists of items. After studying each single list, subjects restudied the list items to enhance learning, or they were asked to recall the items. Following restudy or retrieval practice of list 1 items, subjects were cued to either forget the list or remember it for an upcoming final test. Experiment 1 employed a free-recall and Experiment 1 a cued-recall procedure on the final memory test. In both experiments, directed forgetting was present in the restudy condition but was absent in the retrieval-practice condition, indicating that retrieval practice can reduce or even eliminate this form of forgetting. The results are consistent with the view that retrieval practice enhances list segregation processes. Such processes may reduce interference between lists and thus reduce directed forgetting.

  19. Energy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride.

    PubMed

    Moriguchi, K; Mitamura, Y; Iwami, J; Hasegawa, Y; Higuchi, N; Murakami, Y; Maeda, H; Yoshimura, F; Nakamura, H; Ohno, N

    2012-11-01

    Tannerella forsythensis (Bacteroides forsythus), an anaerobic Gram-negative species of bacteria that plays a role in the progression of periodontal disease, has a unique bacterial protein profile. It is characterized by two unique protein bands with molecular weights of more than 200 kDa. It also is known to have a typical surface layer (S-layer) consisting of regularly arrayed subunits outside the outer membrane. We examined the relationship between high molecular weight proteins and the S-layer using electron microscopic immunolabeling with chemical fixation and an antigen retrieval procedure consisting of heating in a microwave oven or autoclave with citraconic anhydride. Immunogold particles were localized clearly at the outermost cell surface. We also used energy-filtering transmission electron microscopy (EFTEM) to visualize 3, 3'-diaminobenzidine tetrahydrochloride (DAB) reaction products after microwave antigen retrieval with 1% citraconic anhydride. The three-window method for electron spectroscopic images (ESI) of nitrogen by the EFTEM reflected the presence of moieties demonstrated by the DAB reaction with horseradish peroxidase (HRP)-conjugated secondary antibodies instead of immunogold particles. The mapping patterns of net nitrogen were restricted to the outermost cell surface.

  20. Graphical Retrieval Method for Orthorhombic Anisotropic Materials

    DTIC Science & Technology

    2010-07-01

    or nanostructures. We limit our discussion in nonchiral materials. To illustrate the key point we adopt multilayer method that has been used to model...every general multilayer is equivalent to a two-homogeneous-layer system and every symmetric multilayer is equivalent to a single homogeneous layer...characterized by an equivalent index and equivalent thickness[15]. In metamaterials, a single unit-cell layer can be considered as a multilayer system

  1. Hybrid projection-reflection method for phase retrieval

    NASA Astrophysics Data System (ADS)

    Bauschke, Heinz H.; Combettes, Patrick L.; Luke, D. Russell

    2003-06-01

    The phase-retrieval problem, fundamental in applied physics and engineering, addresses the question of how to determine the phase of a complex-valued function from modulus data and additional a priori information. Recently we identified two important methods for phase retrieval, namely, Fienup's basic input-output and hybrid input-output (HIO) algorithms, with classical convex projection methods and suggested that further connections between convex optimization and phase retrieval should be explored. Following up on this work, we introduce a new projection-based method, termed the hybrid projection-reflection (HPR) algorithm, for solving phase-retrieval problems featuring nonnegativity constraints in the object domain. Motivated by properties of the HPR algorithm for convex constraints, we recommend an error measure studied by Fienup more than 20 years ago. This error measure, which has received little attention in the literature, lends itself to an easily implementable stopping criterion. In numerical experiments we found the HPR algorithm to be a competitive alternative to the HIO algorithm and the stopping criterion to be reliable and robust.

  2. Image Retrieval Method for Multiscale Objects from Optical Colonoscopy Images

    PubMed Central

    Sakanashi, Hidenori; Takahashi, Eiichi; Murakawa, Masahiro; Aoki, Hiroshi; Takeuchi, Ken; Suzuki, Yasuo

    2017-01-01

    Optical colonoscopy is the most common approach to diagnosing bowel diseases through direct colon and rectum inspections. Periodic optical colonoscopy examinations are particularly important for detecting cancers at early stages while still treatable. However, diagnostic accuracy is highly dependent on both the experience and knowledge of the medical doctor. Moreover, it is extremely difficult, even for specialist doctors, to detect the early stages of cancer when obscured by inflammations of the colonic mucosa due to intractable inflammatory bowel diseases, such as ulcerative colitis. Thus, to assist the UC diagnosis, it is necessary to develop a new technology that can retrieve similar cases of diagnostic target image from cases in the past that stored the diagnosed images with various symptoms of colonic mucosa. In order to assist diagnoses with optical colonoscopy, this paper proposes a retrieval method for colonoscopy images that can cope with multiscale objects. The proposed method can retrieve similar colonoscopy images despite varying visible sizes of the target objects. Through three experiments conducted with real clinical colonoscopy images, we demonstrate that the method is able to retrieve objects of any visible size and any location at a high level of accuracy. PMID:28255295

  3. Generalized method for retrieving effective parameters of anisotropic metamaterials.

    PubMed

    Castanié, A; Mercier, J-F; Félix, S; Maurel, A

    2014-12-01

    Electromagnetic or acoustic metamaterials can be described in terms of equivalent effective, in general anisotropic, media and several techniques exist to determine the effective permeability and permittivity (or effective mass density and bulk modulus in the context of acoustics). Among these techniques, retrieval methods use the measured reflection and transmission coefficients (or scattering coefficients) for waves incident on a metamaterial slab containing few unit cells. Until now, anisotropic effective slabs have been considered in the literature but they are limited to the case where one of the axes of anisotropy is aligned with the slab interface. We propose an extension to arbitrary orientations of the principal axes of anisotropy and oblique incidence. The retrieval method is illustrated in the electromagnetic case for layered media, and in the acoustic case for array of tilted elliptical particles.

  4. Detection of peste des petits ruminants virus antigen using immunofiltration and antigen-competition ELISA methods.

    PubMed

    Raj, G Dhinakar; Rajanathan, T M C; Kumar, C Senthil; Ramathilagam, G; Hiremath, Geetha; Shaila, M S

    2008-06-22

    Peste des petits ruminants (PPR) is one of the most economically important diseases affecting sheep and goats in India. An immunofiltration-based test has been developed using either mono-specific serum/monoclonal antibodies (mAb) prepared against a recombinant truncated nucleocapsid protein of rinderpest virus (RPV) cross-reactive with PPR virus. This method consists of coating ocular swab eluate from suspected animals onto a nitrocellulose membrane housed in a plastic module, which is allowed to react with suitable dilutions of a mAb or a mono-specific polyclonal antibody. The antigen-antibody complex formed on the membrane is then detected by protein A-colloidal gold conjugate, which forms a pink colour. In the immunofiltration test, concordant results were obtained using either PPRV mAb or mono-specific serum. Another test, an antigen-competition ELISA which relies on the competition between plate-coated recombinant truncated 'N' protein of RPV and the PPRV 'N' protein present in ocular swab eluates (sample) for binding to the mono-specific antibody against N protein of RPV (in liquid phase) was developed. The cut-off value for this test was established using reverse transcription polymerase chain reaction (RT-PCR) positive and negative oculo-nasal swab samples. Linear correlation between percent inhibition (PI) values in antigen-competition ELISA and virus infectivity titres was 0.992. Comparison of the immunofiltration test with the antigen-competition ELISA yielded a sensitivity of 80% and specificity of 100%. These two tests can serve as a screening (immunofiltration) and confirmatory (antigen-competition ELISA) test, respectively, in the diagnosis of PPR in sheep or goats.

  5. Improved retrievals of the optical properties of cirrus clouds by a combination of lidar methods.

    PubMed

    Cadet, Bertrand; Giraud, Vincent; Haeffelin, Martial; Keckhut, Philippe; Rechou, Anne; Baldy, Serge

    2005-03-20

    We focus on improvement of the retrieval of optical properties of cirrus clouds by combining two lidar methods. We retrieve the cloud's optical depth by using independently the molecular backscattering profile below and above the cloud [molecular integration (MI) method] and the backscattering profile inside the cloud with an a priori effective lidar ratio [particle integration (PI) method]. When the MI method is reliable, the combined MI-PI method allows us to retrieve the optimal effective lidar ratio. We compare these results with Raman lidar retrievals. We then use the derived optimal effective lidar ratio for retrieval with the PI method for situations in which the MI method cannot be applied.

  6. Radial immunodiffusion: a simple and rapid method for detection of Marek's disease antigen(s).

    PubMed

    Marquardt, W W

    1972-05-01

    A qualitative radial immunodiffusion technique is described which detects antigen(s) in feathers from live or dead chickens infected with Marek's disease herpesvirus. Antiserum, which is incorporated into a support medium, reacts with antigen(s) in the feather tip producing a radial precipitin ring. Antigen(s) was detected in 93.3% of experimentally inoculated chickens 21 days postinoculation and in 100% of infected birds subsequently tested through 6 weeks. No antigen was detectable in the feathers of uninoculated control chickens. The technique is simple and rapid to perform. Positive tests could be detected after 1 to 2 hours of incubation. Antigen detection by the radial immunodiffusion test correlated well with other criteria of infection. This technique should have application as a laboratory research tool and as an adjunct for a rapid flock diagnosis of Marek's disease.

  7. Effects of antigen retrieval by microwave heating in formalin-fixed tissue sections on a broad panel of antibodies.

    PubMed

    von Wasielewski, R; Werner, M; Nolte, M; Wilkens, L; Georgii, A

    1994-09-01

    Formaldehyde fixation of biopsy specimens for routine purposes has often been held responsible for the poor reproducibility of immunohistochemical studies. Recently, antigen retrieval (AGR) using microwave irradiation was described as a potential tool to enhance immunostaining. A comparison of conventional staining and staining after microwave heating was performed for 52 markers, using tissues fixed in formaldehyde for 24 h, 1 to 6 weeks and 3 years respectively, as well as consultant case material. After adequate duration of fixation (24 h), only a few markers (17%) showed better results after AGR, but this percentage was increased to 50% when tissues were fixed for longer periods. Maximal enhancement was obtained in the group of consultant cases (58% of tested markers demonstrated better staining results), in which the period of fixation and tissue processing was unknown. To achieve reliable enhancement with AGR, continuous heating (100 degrees C) should not be shorter than 20 min. In conclusion, AGR may become the most important tool to simplify and equalize immunohistochemical techniques, if critically evaluated.

  8. Mathematical, Logical, and Formal Methods in Information Retrieval: An Introduction to the Special Issue.

    ERIC Educational Resources Information Center

    Crestani, Fabio; Dominich, Sandor; Lalmas, Mounia; van Rijsbergen, Cornelis Joost

    2003-01-01

    Discusses the importance of research on the use of mathematical, logical, and formal methods in information retrieval to help enhance retrieval effectiveness and clarify underlying concepts of information retrieval. Highlights include logic; probability; spaces; and future research needs. (Author/LRW)

  9. Robustness of phase retrieval methods in x-ray phase contrast imaging: A comparison

    SciTech Connect

    Yan, Aimin; Wu, Xizeng; Liu, Hong

    2011-09-15

    Purpose: The robustness of the phase retrieval methods is of critical importance for limiting and reducing radiation doses involved in x-ray phase contrast imaging. This work is to compare the robustness of two phase retrieval methods by analyzing the phase maps retrieved from the experimental images of a phantom. Methods: Two phase retrieval methods were compared. One method is based on the transport of intensity equation (TIE) for phase contrast projections, and the TIE-based method is the most commonly used method for phase retrieval in the literature. The other is the recently developed attenuation-partition based (AP-based) phase retrieval method. The authors applied these two methods to experimental projection images of an air-bubble wrap phantom for retrieving the phase map of the bubble wrap. The retrieved phase maps obtained by using the two methods are compared. Results: In the wrap's phase map retrieved by using the TIE-based method, no bubble is recognizable, hence, this method failed completely for phase retrieval from these bubble wrap images. Even with the help of the Tikhonov regularization, the bubbles are still hardly visible and buried in the cluttered background in the retrieved phase map. The retrieved phase values with this method are grossly erroneous. In contrast, in the wrap's phase map retrieved by using the AP-based method, the bubbles are clearly recovered. The retrieved phase values with the AP-based method are reasonably close to the estimate based on the thickness-based measurement. The authors traced these stark performance differences of the two methods to their different techniques employed to deal with the singularity problem involved in the phase retrievals. Conclusions: This comparison shows that the conventional TIE-based phase retrieval method, regardless if Tikhonov regularization is used or not, is unstable against the noise in the wrap's projection images, while the AP-based phase retrieval method is shown in these

  10. Identification of 5-bromo-2'-deoxyuridine-labeled cells during mouse spermatogenesis by heat-induced antigen retrieval in lectin staining and immunohistochemistry.

    PubMed

    Wakayama, Tomohiko; Nakata, Hiroki; Kumchantuek, Tewarat; Gewaily, Mahmoud Saad; Iseki, Shoichi

    2015-03-01

    DNA replication occurs during S-phase in spermatogonia and preleptotene spermatocytes during spermatogenesis. 5-Bromo-2'-deoxyuridine (BrdU) is incorporated into synthesized DNA and is detectable in the nucleus by immunohistochemistry (IHC). To identify BrdU-labeled spermatogenic cells, the spermatogenic stages must be determined by visualizing acrosomes and detecting cell type-specific marker molecules in the seminiferous tubules. However, the antibody reaction with BrdU routinely requires denaturation of the DNA, which is achieved by pretreating tissue sections with hydrochloric acid; however, this commonly interferes with further histochemical approaches. Therefore, we examined optimal methods for pretreating paraffin sections of the mouse testis to detect incorporated BrdU by an antibody and, at the same time, visualize acrosomes with peanut agglutinin (PNA) or detect several marker molecules with antibodies. We found that the use of heat-induced antigen retrieval (HIAR), which consisted of heating at 95C in 20 mM Tris-HCl buffer (pH 9.0) for 15 min, was superior to the use of 2 N hydrochloric acid for 90 min at room temperature in terms of the quality of subsequent PNA-lectin histochemistry with double IHC for BrdU and an appropriate stage marker protein. With this method, we identified BrdU-labeled spermatogenic cells during mouse spermatogenesis as A1 spermatogonia through to preleptotene spermatocytes.

  11. A new method of content based medical image retrieval and its applications to CT imaging sign retrieval.

    PubMed

    Ma, Ling; Liu, Xiabi; Gao, Yan; Zhao, Yanfeng; Zhao, Xinming; Zhou, Chunwu

    2017-02-01

    This paper proposes a new method of content based medical image retrieval through considering fused, context-sensitive similarity. Firstly, we fuse the semantic and visual similarities between the query image and each image in the database as their pairwise similarities. Then, we construct a weighted graph whose nodes represent the images and edges measure their pairwise similarities. By using the shortest path algorithm over the weighted graph, we obtain a new similarity measure, context-sensitive similarity measure, between the query image and each database image to complete the retrieval process. Actually, we use the fused pairwise similarity to narrow down the semantic gap for obtaining a more accurate pairwise similarity measure, and spread it on the intrinsic data manifold to achieve the context-sensitive similarity for a better retrieval performance. The proposed method has been evaluated on the retrieval of the Common CT Imaging Signs of Lung Diseases (CISLs) and achieved not only better retrieval results but also the satisfactory computation efficiency.

  12. Comparison of the Assimilation of Compact Phase Space Retrievals (CPSRs) with Conventional Retrieval Assimilation Methods for MOPITT CO in WRF-Chem/DART

    NASA Astrophysics Data System (ADS)

    Mizzi, A. P.; Arellano, A. F.; Edwards, D. P.; Anderson, J. L.

    2015-12-01

    We introduced "compact phase space retrievals" (CPSRs) for assimilating atmospheric composition observations in Mizzi et al. (2015). CPSRs address many of the challenges associated with assimilating retrievals by: (i) removing the contribution of the retrieval prior, (ii) diagonalizing the associated error covariance, and (iii) compacting the phase space retrievals. Mizzi et al. (2015) showed that the CPSRs reduced assimilation computational costs by approximately one-third while maintaining the relevant forecast skill metrics. In this paper, we examine phase space retrievals and compare CPSRs to more conventional retrieval assimilation methods. Specifically we compare the assimilation of CPSRs with that of (i) raw retrievals where the observation error is based on the diagonal of the retrieval error covariance, (ii) quasi-optimal retrievals (QORs) where the observation error is determined as (i), and (iii) QORs where the observation error is based on an SVD-based transform of the error covariance. Method (i) is the easiest to interpret because the assimilation occurs in retrieval space. Method (ii) explicitly removes the retrieval prior term's contribution but is more difficult to interpret because the assimilated observations are residuals. Finally, method (iii) is the phase space retrieval algorithm of Migliorini et al. (2008). We consider two variants: (i) withholding the inverse square scaling and (ii) using phase space filtering for optimization. We apply those methods to month-long forecast/assimilation experiments with continuous cycling.

  13. Rapid Retrieval of Lung Nodule CT Images Based on Hashing and Pruning Methods.

    PubMed

    Pan, Ling; Qiang, Yan; Yuan, Jie; Wu, Lidong

    2016-01-01

    The similarity-based retrieval of lung nodule computed tomography (CT) images is an important task in the computer-aided diagnosis of lung lesions. It can provide similar clinical cases for physicians and help them make reliable clinical diagnostic decisions. However, when handling large-scale lung images with a general-purpose computer, traditional image retrieval methods may not be efficient. In this paper, a new retrieval framework based on a hashing method for lung nodule CT images is proposed. This method can translate high-dimensional image features into a compact hash code, so the retrieval time and required memory space can be reduced greatly. Moreover, a pruning algorithm is presented to further improve the retrieval speed, and a pruning-based decision rule is presented to improve the retrieval precision. Finally, the proposed retrieval method is validated on 2,450 lung nodule CT images selected from the public Lung Image Database Consortium (LIDC) database. The experimental results show that the proposed pruning algorithm effectively reduces the retrieval time of lung nodule CT images and improves the retrieval precision. In addition, the retrieval framework is evaluated by differentiating benign and malignant nodules, and the classification accuracy can reach 86.62%, outperforming other commonly used classification methods.

  14. Rapid Retrieval of Lung Nodule CT Images Based on Hashing and Pruning Methods

    PubMed Central

    Pan, Ling; Yuan, Jie; Wu, Lidong

    2016-01-01

    The similarity-based retrieval of lung nodule computed tomography (CT) images is an important task in the computer-aided diagnosis of lung lesions. It can provide similar clinical cases for physicians and help them make reliable clinical diagnostic decisions. However, when handling large-scale lung images with a general-purpose computer, traditional image retrieval methods may not be efficient. In this paper, a new retrieval framework based on a hashing method for lung nodule CT images is proposed. This method can translate high-dimensional image features into a compact hash code, so the retrieval time and required memory space can be reduced greatly. Moreover, a pruning algorithm is presented to further improve the retrieval speed, and a pruning-based decision rule is presented to improve the retrieval precision. Finally, the proposed retrieval method is validated on 2,450 lung nodule CT images selected from the public Lung Image Database Consortium (LIDC) database. The experimental results show that the proposed pruning algorithm effectively reduces the retrieval time of lung nodule CT images and improves the retrieval precision. In addition, the retrieval framework is evaluated by differentiating benign and malignant nodules, and the classification accuracy can reach 86.62%, outperforming other commonly used classification methods. PMID:27995140

  15. A TEMPERATURE AND ABUNDANCE RETRIEVAL METHOD FOR EXOPLANET ATMOSPHERES

    SciTech Connect

    Madhusudhan, N.; Seager, S.

    2009-12-10

    We present a new method to retrieve molecular abundances and temperature profiles from exoplanet atmosphere photometry and spectroscopy. We run millions of one-dimensional (1D) atmosphere models in order to cover the large range of allowed parameter space. In order to run such a large number of models, we have developed a parametric pressure-temperature (P-T) profile coupled with line-by-line radiative transfer, hydrostatic equilibrium, and energy balance, along with prescriptions for non-equilibrium molecular composition and energy redistribution. The major difference from traditional 1D radiative transfer models is the parametric P-T profile, which essentially means adopting energy balance only at the top of the atmosphere and not in each layer. We see the parametric P-T model as a parallel approach to the traditional exoplanet atmosphere models that rely on several free parameters to encompass unknown absorbers and energy redistribution. The parametric P-T profile captures the basic physical features of temperature structures in planetary atmospheres (including temperature inversions), and fits a wide range of published P-T profiles, including those of solar system planets. We apply our temperature and abundance retrieval method to the atmospheres of two transiting exoplanets, HD 189733b and HD 209458b, which have the best Spitzer and Hubble Space Telescope data available. For HD 189733b, we find efficient day-night redistribution of energy in the atmosphere, and molecular abundance constraints confirming the presence of H{sub 2}O, CO, CH{sub 4}, and CO{sub 2}. For HD 209458b, we confirm and constrain the dayside thermal inversion in an average 1D temperature profile. We also report independent detections of H{sub 2}O, CO, CH{sub 4}, and CO{sub 2} on the dayside of HD 209458b, based on six-channel Spitzer photometry. We report constraints for HD 189733b due to individual data sets separately; a few key observations are variable in different data sets at similar

  16. Quantitative cell imaging using single beam phase retrieval method

    NASA Astrophysics Data System (ADS)

    Anand, Arun; Chhaniwal, Vani; Javidi, Bahram

    2011-06-01

    Quantitative three-dimensional imaging of cells can provide important information about their morphology as well as their dynamics, which will be useful in studying their behavior under various conditions. There are several microscopic techniques to image unstained, semi-transparent specimens, by converting the phase information into intensity information. But most of the quantitative phase contrast imaging techniques is realized either by using interference of the object wavefront with a known reference beam or using phase shifting interferometry. A two-beam interferometric method is challenging to implement especially with low coherent sources and it also requires a fine adjustment of beams to achieve high contrast fringes. In this letter, the development of a single beam phase retrieval microscopy technique for quantitative phase contrast imaging of cells using multiple intensity samplings of a volume speckle field in the axial direction is described. Single beam illumination with multiple intensity samplings provides fast convergence and a unique solution of the object wavefront. Three-dimensional thickness profiles of different cells such as red blood cells and onion skin cells were reconstructed using this technique with an axial resolution of the order of several nanometers.

  17. Immunolocalization of MAP-2 in routinely formalin-fixed, paraffin-embedded guinea pig brain sections using microwave irradiation: a comparison of different combinations of antibody clones and antigen retrieval buffer solutions.

    PubMed

    Kan, Robert K; Pleva, Christina M; Hamilton, Tracey A; Petrali, John P

    2005-04-01

    The present study was designed to evaluate the efficacy of different microwave pretreatment methods to retrieve microtubule-associated protein 2 (MAP-2) immunoreactivity in formalin-fixed, paraffin-embedded guinea pig brain sections. Brain sections, microwave pretreated in boiling sodium citrate, citric acid, Tris hydrochloride, and EDTA buffers of pH 4, 6, and 8, were labeled with four different clones of MAP-2 monoclonal antibodies. No MAP-2 immunoreactivity was observed in control sections processed without microwave pretreatment. Optimal MAP-2 immunoreactivity was observed only when MAP-2 antibody clone AP18 was used in conjunction with citric acid buffer of pH 6.0. Using this combination, brain sections from nerve agent soman-exposed guinea pigs were found to exhibit marked reduction in MAP-2 immunostaining in the hippocampus. These observations suggest that the clone of the antibody in addition to the type and pH of antigen retrieval (AR) solution are important variables to be considered for establishing an optimal AR technique. When studying counterpart antigens of species other than that to which the antibodies were originally raised, different antibody clones must be tested in combination with different microwave-assisted AR (MAR) methods. This MAR method makes it possible to conduct retrospective studies on archival guinea pig brain paraffin blocks to evaluate changes in neuronal MAP-2 expression as a consequence of chemical warfare nerve agent toxicity.

  18. Evaluation of contents-based image retrieval methods for a database of logos on drug tablets

    NASA Astrophysics Data System (ADS)

    Geradts, Zeno J.; Hardy, Huub; Poortman, Anneke; Bijhold, Jurrien

    2001-02-01

    In this research an evaluation has been made of the different ways of contents based image retrieval of logos of drug tablets. On a database of 432 illicitly produced tablets (mostly containing MDMA), we have compared different retrieval methods. Two of these methods were available from commercial packages, QBIC and Imatch, where the implementation of the contents based image retrieval methods are not exactly known. We compared the results for this database with the MPEG-7 shape comparison methods, which are the contour-shape, bounding box and region-based shape methods. In addition, we have tested the log polar method that is available from our own research.

  19. A Method for Retrieving Ground Flash Fraction from Satellite Lightning Imager Data

    NASA Technical Reports Server (NTRS)

    Koshak, William J.

    2009-01-01

    A general theory for retrieving the fraction of ground flashes in N lightning observed by a satellite-based lightning imager is provided. An "exponential model" is applied as a physically reasonable constraint to describe the measured optical parameter distributions, and population statistics (i.e., mean, variance) are invoked to add additional constraints to the retrieval process. The retrieval itself is expressed in terms of a Bayesian inference, and the Maximum A Posteriori (MAP) solution is obtained. The approach is tested by performing simulated retrievals, and retrieval error statistics are provided. The ability to retrieve ground flash fraction has important benefits to the atmospheric chemistry community. For example, using the method to partition the existing satellite global lightning climatology into separate ground and cloud flash climatologies will improve estimates of lightning nitrogen oxides (NOx) production; this in turn will improve both regional air quality and global chemistry/climate model predictions.

  20. The Monte Carlo Method and the Evaluation of Retrieval System Performance.

    ERIC Educational Resources Information Center

    Burgin, Robert

    1999-01-01

    Introduces the Monte Carlo method which is shown to represent an attractive alternative to the hypergeometric model for identifying the levels at which random retrieval performance is exceeded in retrieval test collections and for overcoming some of the limitations of the hypergeometric model. Practical matters to consider when employing the Monte…

  1. Spatial-light-modulator-based adaptive optical system for the use of multiple phase retrieval methods.

    PubMed

    Lingel, Christian; Haist, Tobias; Osten, Wolfgang

    2016-12-20

    We propose an adaptive optical setup using a spatial light modulator (SLM), which is suitable to perform different phase retrieval methods with varying optical features and without mechanical movement. By this approach, it is possible to test many different phase retrieval methods and their parameters (optical and algorithmic) using one stable setup and without hardware adaption. We show exemplary results for the well-known transport of intensity equation (TIE) method and a new iterative adaptive phase retrieval method, where the object phase is canceled by an inverse phase written into part of the SLM. The measurement results are compared to white light interferometric measurements.

  2. Lensless imaging from diffraction intensity measurements by use of a noniterative phase-retrieval method.

    PubMed

    Nakajima, Nobuharu

    2004-03-10

    A method of reconstructing the complex amplitude of an object that is illuminated by a coherent wave from its Fresnel diffraction patterns is proposed for high-frequency wave phenomena such as x-rays and electron waves. A noniterative phase-retrieval method that uses a Gaussian filter is employed here, and it is shown that the object's illumination with amplitude distribution in the Fraunhofer diffraction pattern of a circular aperture can be used as a substitute for the Gaussian filter. This method has an advantage over other noniterative phase-retrieval methods in that it can retrieve phase vortices.

  3. 3D model retrieval method based on mesh segmentation

    NASA Astrophysics Data System (ADS)

    Gan, Yuanchao; Tang, Yan; Zhang, Qingchen

    2012-04-01

    In the process of feature description and extraction, current 3D model retrieval algorithms focus on the global features of 3D models but ignore the combination of global and local features of the model. For this reason, they show less effective performance to the models with similar global shape and different local shape. This paper proposes a novel algorithm for 3D model retrieval based on mesh segmentation. The key idea is to exact the structure feature and the local shape feature of 3D models, and then to compares the similarities of the two characteristics and the total similarity between the models. A system that realizes this approach was built and tested on a database of 200 objects and achieves expected results. The results show that the proposed algorithm improves the precision and the recall rate effectively.

  4. Retrieving Land Surface Temperature from Hyperspectral Thermal Infrared Data Using a Multi-Channel Method

    PubMed Central

    Zhong, Xinke; Huo, Xing; Ren, Chao; Labed, Jelila; Li, Zhao-Liang

    2016-01-01

    Land Surface Temperature (LST) is a key parameter in climate systems. The methods for retrieving LST from hyperspectral thermal infrared data either require accurate atmospheric profile data or require thousands of continuous channels. We aim to retrieve LST for natural land surfaces from hyperspectral thermal infrared data using an adapted multi-channel method taking Land Surface Emissivity (LSE) properly into consideration. In the adapted method, LST can be retrieved by a linear function of 36 brightness temperatures at Top of Atmosphere (TOA) using channels where LSE has high values. We evaluated the adapted method using simulation data at nadir and satellite data near nadir. The Root Mean Square Error (RMSE) of the LST retrieved from the simulation data is 0.90 K. Compared with an LST product from the Spinning Enhanced Visible and Infrared Imager (SEVIRI) on Meteosat, the error in the LST retrieved from the Infared Atmospheric Sounding Interferometer (IASI) is approximately 1.6 K. The adapted method can be used for the near-real-time production of an LST product and to provide the physical method to simultaneously retrieve atmospheric profiles, LST, and LSE with a first-guess LST value. The limitations of the adapted method are that it requires the minimum LSE in the spectral interval of 800–950 cm−1 larger than 0.95 and it has not been extended for off-nadir measurements. PMID:27187408

  5. A phase retrieval method of interferograms add-subtracting based on two-step phase shifting.

    PubMed

    Xu, Yuanyuan; Wang, Yawei; Ji, Ying; Jin, Weifeng; Bu, Min; Shang, Xuefu

    2014-01-01

    A phase retrieval method is introduced in quantitative phase imaging (QPI) based on two-step phase-shifting technique. By acquiring two measured interferograms and calculating the addition and subtraction between them, the quantitative phase information can be directly retrieved. This method is illustrated by both theory and simulation experiments of a ball. The results of the simulation and the experiment of the red blood cell show a good agreement, demonstrating its application for studying cells.

  6. Quantitative interferometric microscopy with two dimensional Hilbert transform based phase retrieval method

    NASA Astrophysics Data System (ADS)

    Wang, Shouyu; Yan, Keding; Xue, Liang

    2017-01-01

    In order to obtain high contrast images and detailed descriptions of label free samples, quantitative interferometric microscopy combining with phase retrieval is designed to obtain sample phase distributions from fringes. As accuracy and efficiency of recovered phases are affected by phase retrieval methods, thus approaches owning higher precision and faster processing speed are still in demand. Here, two dimensional Hilbert transform based phase retrieval method is adopted in cellular phase imaging, it not only reserves more sample specifics compared to classical fast Fourier transform based method, but also overcomes disadvantages of traditional algorithm according to Hilbert transform which is a one dimensional processing causing phase ambiguities. Both simulations and experiments are provided, proving the proposed phase retrieval approach can acquire quantitative sample phases with high accuracy and fast speed.

  7. Brain CT image similarity retrieval method based on uncertain location graph.

    PubMed

    Pan, Haiwei; Li, Pengyuan; Li, Qing; Han, Qilong; Feng, Xiaoning; Gao, Linlin

    2014-03-01

    A number of brain computed tomography (CT) images stored in hospitals that contain valuable information should be shared to support computer-aided diagnosis systems. Finding the similar brain CT images from the brain CT image database can effectively help doctors diagnose based on the earlier cases. However, the similarity retrieval for brain CT images requires much higher accuracy than the general images. In this paper, a new model of uncertain location graph (ULG) is presented for brain CT image modeling and similarity retrieval. According to the characteristics of brain CT image, we propose a novel method to model brain CT image to ULG based on brain CT image texture. Then, a scheme for ULG similarity retrieval is introduced. Furthermore, an effective index structure is applied to reduce the searching time. Experimental results reveal that our method functions well on brain CT images similarity retrieval with higher accuracy and efficiency.

  8. Investigating the Bag-of-Words Method for 3D Shape Retrieval

    NASA Astrophysics Data System (ADS)

    Li, Xiaolan; Godil, Afzal

    2010-12-01

    This paper investigates the capabilities of the Bag-of-Words (BWs) method in the 3D shape retrieval field. The contributions of this paper are (1) the 3D shape retrieval task is categorized from different points of view: specific versus generic, partial-to-global retrieval (PGR) versus global-to-global retrieval (GGR), and articulated versus nonarticulated (2) the spatial information, represented as concentric spheres, is integrated into the framework to improve the discriminative ability (3) the analysis of the experimental results on Purdue Engineering Benchmark (PEB) reveals that some properties of the BW approach make it perform better on the PGR task than the GGR task (4) the BW approach is evaluated on nonarticulated database PEB and articulated database McGill Shape Benchmark (MSB) and compared to other methods.

  9. Adjusted Levenberg-Marquardt method application to methene retrieval from IASI/METOP spectra

    NASA Astrophysics Data System (ADS)

    Khamatnurova, Marina; Gribanov, Konstantin

    2016-04-01

    Levenberg-Marquardt method [1] with iteratively adjusted parameter and simultaneous evaluation of averaging kernels together with technique of parameters selection are developed and applied to the retrieval of methane vertical profiles in the atmosphere from IASI/METOP spectra. Retrieved methane vertical profiles are then used for calculation of total atmospheric column amount. NCEP/NCAR reanalysis data provided by ESRL (NOAA, Boulder,USA) [2] are taken as initial guess for retrieval algorithm. Surface temperature, temperature and humidity vertical profiles are retrieved before methane vertical profile retrieval for each selected spectrum. Modified software package FIRE-ARMS [3] were used for numerical experiments. To adjust parameters and validate the method we used ECMWF MACC reanalysis data [4]. Methane columnar values retrieved from cloudless IASI spectra demonstrate good agreement with MACC columnar values. Comparison is performed for IASI spectra measured in May of 2012 over Western Siberia. Application of the method for current IASI/METOP measurements are discussed. 1.Ma C., Jiang L. Some Research on Levenberg-Marquardt Method for the Nonlinear Equations // Applied Mathematics and Computation. 2007. V.184. P. 1032-1040 2.http://www.esrl.noaa.gov/psdhttp://www.esrl.noaa.gov/psd 3.Gribanov K.G., Zakharov V.I., Tashkun S.A., Tyuterev Vl.G.. A New Software Tool for Radiative Transfer Calculations and its application to IMG/ADEOS data // JQSRT.2001.V.68.№ 4. P. 435-451. 4.http://www.ecmwf.int/http://www.ecmwf.int

  10. A method for retrieving clouds with satellite infrared radiances using the particle filter

    NASA Astrophysics Data System (ADS)

    Xu, Dongmei; Auligné, Thomas; Descombes, Gaël; Snyder, Chris

    2016-11-01

    Ensemble-based techniques have been widely utilized in estimating uncertainties in various problems of interest in geophysical applications. A new cloud retrieval method is proposed based on the particle filter (PF) by using ensembles of cloud information in the framework of Gridpoint Statistical Interpolation (GSI) system. The PF cloud retrieval method is compared with the Multivariate Minimum Residual (MMR) method that was previously established and verified. Cloud retrieval experiments involving a variety of cloudy types are conducted with the PF and MMR methods with measurements of infrared radiances on multi-sensors onboard both geostationary and polar satellites, respectively. It is found that the retrieved cloud masks with both methods are consistent with other independent cloud products. MMR is prone to producing ambiguous small-fraction clouds, while PF detects clearer cloud signals, yielding closer heights of cloud top and cloud base to other references. More collections of small-fraction particles are able to effectively estimate the semi-transparent high clouds. It is found that radiances with high spectral resolutions contribute to quantitative cloud top and cloud base retrievals. In addition, a different way of resolving the filtering problem over each model grid is tested to better aggregate the weights with all available sensors considered, which is proven to be less constrained by the ordering of sensors. Compared to the MMR method, the PF method is overall more computationally efficient, and the cost of the model grid-based PF method scales more directly with the number of computing nodes.

  11. Two Surface Temperature Retrieval Methods Compared Over Agricultural Lands

    NASA Technical Reports Server (NTRS)

    French, Andrew N.; Schmugge, Thomas J.; Jacob, Frederic; Ogawa, Kenta; Houser, Paul R. (Technical Monitor)

    2002-01-01

    Accurate, spatially distributed surface temperatures are required for modeling evapotranspiration (ET) over agricultural fields under wide ranging conditions, including stressed and unstressed vegetation. Modeling approaches that use surface temperature observations, however, have the burden of estimating surface emissivities. Emissivity estimation, the subject of much recent research, is facilitated by observations in multiple thermal infrared bands. But it is nevertheless a difficult task. Using observations from a multiband thermal sensor, the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), estimated surface emissivities and temperatures are retrieved in two different ways: the temperature emissivity separation approach (TES) and the normalized emissivity approach (NEM). Both rely upon empirical relationships, but the assumed relationships are different. TES relies upon a relationship between the minimum spectral emissivity and the range of observed emissivities. NEM relies upon an assumption that at least one thermal band has a pre-determined emissivity (close to 1.0). The benefits and consequences of each approach will be demonstrated for two different landscapes: one in central Oklahoma, USA and another in southern New Mexico.

  12. The Continuous Monitoring of Desert Dust using an Infrared-based Dust Detection and Retrieval Method

    NASA Technical Reports Server (NTRS)

    Duda, David P.; Minnis, Patrick; Trepte, Qing; Sun-Mack, Sunny

    2006-01-01

    Airborne dust and sand are significant aerosol sources that can impact the atmospheric and surface radiation budgets. Because airborne dust affects visibility and air quality, it is desirable to monitor the location and concentrations of this aerosol for transportation and public health. Although aerosol retrievals have been derived for many years using visible and near-infrared reflectance measurements from satellites, the detection and quantification of dust from these channels is problematic over bright surfaces, or when dust concentrations are large. In addition, aerosol retrievals from polar orbiting satellites lack the ability to monitor the progression and sources of dust storms. As a complement to current aerosol dust retrieval algorithms, multi-spectral thermal infrared (8-12 micron) data from the Moderate Resolution Imaging Spectroradiometer (MODIS) and the Meteosat-8 Spinning Enhanced Visible and Infrared Imager (SEVIRI) are used in the development of a prototype dust detection method and dust property retrieval that can monitor the progress of Saharan dust fields continuously, both night and day. The dust detection method is incorporated into the processing of CERES (Clouds and the Earth s Radiant Energy System) aerosol retrievals to produce dust property retrievals. Both MODIS (from Terra and Aqua) and SEVERI data are used to develop the method.

  13. Neighbourhood search feature selection method for content-based mammogram retrieval.

    PubMed

    Chandy, D Abraham; Christinal, A Hepzibah; Theodore, Alwyn John; Selvan, S Easter

    2017-03-01

    Content-based image retrieval plays an increasing role in the clinical process for supporting diagnosis. This paper proposes a neighbourhood search method to select the near-optimal feature subsets for the retrieval of mammograms from the Mammographic Image Analysis Society (MIAS) database. The features based on grey level cooccurrence matrix, Daubechies-4 wavelet, Gabor, Cohen-Daubechies-Feauveau 9/7 wavelet and Zernike moments are extracted from mammograms available in the MIAS database to form the combined or fused feature set for testing various feature selection methods. The performance of feature selection methods is evaluated using precision, storage requirement and retrieval time measures. Using the proposed method, a significant improvement is achieved in mean precision rate and feature dimension. The results show that the proposed method outperforms the state-of-the-art feature selection methods.

  14. Simple, flexible, and accurate phase retrieval method for generalized phase-shifting interferometry.

    PubMed

    Yatabe, Kohei; Ishikawa, Kenji; Oikawa, Yasuhiro

    2017-01-01

    This paper presents a non-iterative phase retrieval method from randomly phase-shifted fringe images. By combining the hyperaccurate least squares ellipse fitting method with the subspace method (usually called the principal component analysis), a fast and accurate phase retrieval algorithm is realized. The proposed method is simple, flexible, and accurate. It can be easily coded without iteration, initial guess, or tuning parameter. Its flexibility comes from the fact that totally random phase-shifting steps and any number of fringe images greater than two are acceptable without any specific treatment. Finally, it is accurate because the hyperaccurate least squares method and the modified subspace method enable phase retrieval with a small error as shown by the simulations. A MATLAB code, which is used in the experimental section, is provided within the paper to demonstrate its simplicity and easiness.

  15. A content-based image retrieval method for optical colonoscopy images based on image recognition techniques

    NASA Astrophysics Data System (ADS)

    Nosato, Hirokazu; Sakanashi, Hidenori; Takahashi, Eiichi; Murakawa, Masahiro

    2015-03-01

    This paper proposes a content-based image retrieval method for optical colonoscopy images that can find images similar to ones being diagnosed. Optical colonoscopy is a method of direct observation for colons and rectums to diagnose bowel diseases. It is the most common procedure for screening, surveillance and treatment. However, diagnostic accuracy for intractable inflammatory bowel diseases, such as ulcerative colitis (UC), is highly dependent on the experience and knowledge of the medical doctor, because there is considerable variety in the appearances of colonic mucosa within inflammations with UC. In order to solve this issue, this paper proposes a content-based image retrieval method based on image recognition techniques. The proposed retrieval method can find similar images from a database of images diagnosed as UC, and can potentially furnish the medical records associated with the retrieved images to assist the UC diagnosis. Within the proposed method, color histogram features and higher order local auto-correlation (HLAC) features are adopted to represent the color information and geometrical information of optical colonoscopy images, respectively. Moreover, considering various characteristics of UC colonoscopy images, such as vascular patterns and the roughness of the colonic mucosa, we also propose an image enhancement method to highlight the appearances of colonic mucosa in UC. In an experiment using 161 UC images from 32 patients, we demonstrate that our method improves the accuracy of retrieving similar UC images.

  16. Mitigating the effect of noise in the hybrid input-output method of phase retrieval.

    PubMed

    Trahan, Russell; Hyland, David

    2013-05-01

    Here a modification to the hybrid input-output (HIO) method of phase retrieval is presented which aides in mitigating the negative effects of low signal-to-noise ratios (SNRs). Various type of interferometers measure diffraction patterns which are used to determine the Fourier transform modulus of an objective. Interferometry often suffers from very low SNRs making phase retrieval difficult because of the sensitivity of most phase retrieval algorithms to local minima. Here we analyze the effect of noise on the HIO method. The result is used as a rationale for the proposed modification to the HIO method. The algorithm presented here introduces a filtering scheme which removes much of the Fourier modulus noise. Examples are shown and the results are compared to the HIO method with and without the proposed modification. Comparisons are also made to other methods of filtering the Fourier modulus noise.

  17. Experimental demonstration of scanning phase retrieval by a noniterative method with a Gaussian-amplitude beam

    NASA Astrophysics Data System (ADS)

    Nakajima, Nobuharu; Yoshino, Masayuki

    2017-01-01

    We present a proof-of-principle experiment of an analytic (noniterative) phase-retrieval method for coherent imaging systems under scanning illumination of a probe beam. This method allows to reconstruct the amplitude and phase distribution of a semi-transparent object over a wide area from intensities measured at three points in the Fourier plane of the object under scanning illumination of a known Gaussian-amplitude beam in the object plane. The present measurement system is very simple in contrast to ones of interferometric techniques, and also the speed of the calculation of phase retrieval in this method is faster than that in iterative algorithms since this method is based on an analytic solution to the phase retrieval. The effectiveness of this method is shown in experimental examples of the object reconstructions of a converging lens and a plastic plate for scratch standards.

  18. Development of surface profile measurement method for ellipsoidal x-ray mirrors using phase retrieval

    NASA Astrophysics Data System (ADS)

    Saitou, Takahiro; Takei, Yoshinori; Mimura, Hidekazu

    2012-09-01

    An ellipsoidal mirror is a promising type of X-ray mirror, because it can focus X-rays to nanometer size with a very large aperture and no chromatic aberration. However, ideal ellipsoidal mirrors have not yet been realized by any manufacturing method. This is partly because there is no evaluation method for its surface figure profile. In this paper, we propose and develop a method for measuring surface figure profile of ellipsoidal mirrors using phase retrieval. An optical design for soft X-ray focusing, the employed phase retrieval method and an experimental optical system specialized for wavefront measurement using a He-Ne laser are reported.

  19. Retrieval of Temperature From a Multiple Channel Rayleigh-Scatter Lidar Using an Optimal Estimation Method

    NASA Astrophysics Data System (ADS)

    Sica, R. J.; Haefele, A.

    2014-12-01

    The measurement of temperature in the middle atmosphere with Rayleigh-scatter lidars is an important technique for assessing atmospheric change. Current retrieval schemes for these temperature have several shortcoming which can be overcome using an optimal estimation method (OEM). OEMs are applied to the retrieval of temperature from Rayleigh-scatter lidar measurements using both single and multiple channel measurements. Forward models are presented that completely characterize the measurement and allow the simultaneous retrieval of temperature, dead time and background. The method allows a full uncertainty budget to be obtained on a per profile basis that includes, in addition to the statistical uncertainties, the smoothing error and uncertainties due to Rayleigh extinction, ozone absorption, the lidar constant, nonlinearity in the counting system, variation of the Rayleigh-scatter cross section with altitude, pressure, acceleration due to gravity and the variation of mean molecular mass with altitude. The vertical resolution of the temperature profile is found at each height, and a quantitative determination is made of the maximum height to which the retrieval is valid. A single temperature profile can be retrieved from measurements with multiple channels that cover different height ranges, vertical resolutions and even different detection methods. The OEM employed is shown to give robust estimates of temperature consistent with previous methods, while requiring minimal computational time. This demonstrated success of lidar temperature retrievals using an OEM opens new possibilities in atmospheric science for measurement integration between active and passive remote sensing instruments. We are currently working on extending our method to simultaneously retrieve water vapour and temperature using Raman-scatter lidar measurements.

  20. Calcium-sensitive immunoaffinity chromatography: Gentle and highly specific retrieval of a scarce plasma antigen, collectin-LK (CL-LK).

    PubMed

    Henriksen, Maiken L; Madsen, Kirstine L; Skjoedt, Karsten; Hansen, Soren

    2014-11-01

    Immunoaffinity chromatography is a powerful fractionation technique that has become indispensable for protein purification and characterization. However, it is difficult to retrieve bound proteins without using harsh or denaturing elution conditions, and the purification of scarce antigens to homogeneity may be impossible due to contamination with abundant antigens. In this study, we purified the scarce, complement-associated plasma protein complex, collectin LK (CL-LK, complex of collectin liver 1 and kidney 1), by immunoaffinity chromatography using a calcium-sensitive anti-collectin-kidney-1 mAb. This antibody was characterized by binding to CL-LK at hypo- and physiological calcium concentrations and dissociated from CK-LK at hyperphysiological concentrations of calcium. We purified CL-LK from plasma to a purity of 41% and a yield of 38%, resulting in a purification factor of more than 88,000 in a single step. To evaluate the efficiency of this new purification scheme, we purified CL-LK using the same calcium-sensitive mAb in combination with acidic elution buffer and by using calcium-dependent anti-CL-K1 mAbs in combination with EDTA elution buffer. We found that calcium-sensitive immunoaffinity chromatography was superior to the traditional immunoaffinity chromatographies and resulted in a nine-fold improvement of the purification factor. The technique is applicable for the purification of proteins in complex mixtures by single-step fractionation without the denaturation of eluted antigens, and it allows for the purification of scarce proteins that would have otherwise been impossible to purify and, hence, to characterize. This technique may also potentially be applied for the purification of proteins that only interact with calcium ions at hyperphysiological concentrations.

  1. von Willebrand factor antigen: a radial immunodiffusion method evaluated and compared with an ELISA method.

    PubMed

    Blann, A D

    1992-06-01

    A new commercial kit method for the quantification of von Willebrand factor antigen (vWFAg) by radial immunodiffusion was compared to an established ELISA technique. Major discrepancies were found between the two methods. The radial immunodiffusion method had poorer intra- and inter-assay coefficients of variation than the ELISA method, although there was adequate inter-method agreement when 100 plasma samples from controls and patients were compared. However, there was a great difference in values obtained for vWFAg in the reference sample supplied with the commercial kit and that obtained directly from the National Institute for Biological Standards and Controls. There were also differences in levels of significance when vWFAg was measured by the two techniques in different clinical groups, and standard deviations were larger when the kit method was used. It is suggested that on scientific and economic grounds, the commercial radial immunodiffusion kit does not offer a competitive advantage over the ELISA method.

  2. A novel method of retrieving the polarization qubits after being transmitted in turbid media

    NASA Astrophysics Data System (ADS)

    Xu, Qiang; Guo, Zhongyi; Tao, Qiangqiang; Jiao, Weiyan; Qu, Shiliang; Gao, Jun

    2015-03-01

    In this paper, we have mainly investigated the reconstruction of the polarization states of the incident light which passed through the turbid atmospheric media. Here, a novel polarization retrieve (PR) method is established using a Monte Carlo simulation algorithm, which is proposed to study the influence of particle scattering. The polarization states of the initial field could be retrieved accurately according to the polarization states of the scattered field and the Mueller matrix. In the case of a single homogeneous medium, the mean error of retrieved polarization is close to zero. We have also applied this method to complex atmospheric environments such as random-sized distribution particles and various index of refraction media. By comparison with the existing polarization maintaining method, the results indicate that the PR method is more applicable to disordered media with relatively larger particles. Moreover, the errors of the retrieved degree of polarization are below 0.11%, and the errors of the retrieved angle of polarization are below 0.54% in our mixed media model. So, this work will be very significant for polarizing quantum secure communication in scattering medium over a long-distance scope.

  3. Evaluation of a content-based retrieval system for blood cell images with automated methods.

    PubMed

    Seng, Woo Chaw; Mirisaee, Seyed Hadi

    2011-08-01

    Content-based image retrieval techniques have been extensively studied for the past few years. With the growth of digital medical image databases, the demand for content-based analysis and retrieval tools has been increasing remarkably. Blood cell image is a key diagnostic tool for hematologists. An automated system that can retrieved relevant blood cell images correctly and efficiently would save the effort and time of hematologists. The purpose of this work is to develop such a content-based image retrieval system. Global color histogram and wavelet-based methods are used in the prototype. The system allows users to search by providing a query image and select one of four implemented methods. The obtained results demonstrate the proposed extended query refinement has the potential to capture a user's high level query and perception subjectivity by dynamically giving better query combinations. Color-based methods performed better than wavelet-based methods with regard to precision, recall rate and retrieval time. Shape and density of blood cells are suggested as measurements for future improvement. The system developed is useful for undergraduate education.

  4. Texture based feature extraction methods for content based medical image retrieval systems.

    PubMed

    Ergen, Burhan; Baykara, Muhammet

    2014-01-01

    The developments of content based image retrieval (CBIR) systems used for image archiving are continued and one of the important research topics. Although some studies have been presented general image achieving, proposed CBIR systems for archiving of medical images are not very efficient. In presented study, it is examined the retrieval efficiency rate of spatial methods used for feature extraction for medical image retrieval systems. The investigated algorithms in this study depend on gray level co-occurrence matrix (GLCM), gray level run length matrix (GLRLM), and Gabor wavelet accepted as spatial methods. In the experiments, the database is built including hundreds of medical images such as brain, lung, sinus, and bone. The results obtained in this study shows that queries based on statistics obtained from GLCM are satisfied. However, it is observed that Gabor Wavelet has been the most effective and accurate method.

  5. A neural network based error correction method for radio occultation electron density retrieval

    NASA Astrophysics Data System (ADS)

    Pham, Viet-Cuong; Juang, Jyh-Ching

    2015-12-01

    Abel inversion techniques have been widely employed to retrieve electron density profiles (EDPs) from radio occultation (RO) measurements, which are available by observing Global Navigation Satellite System (GNSS) satellites from low-earth-orbit (LEO) satellites. It is well known that the ordinary Abel inversion might introduce errors in the retrieval of EDPs when the spherical symmetry assumption is violated. The error, however, is case-dependent; therefore it is desirable to associate an error index or correction coefficient with respect to each retrieved EDP. Several error indices have been proposed but they only deal with electron density at the F2 peak and suffer from some drawbacks. In this paper we propose an artificial neural network (ANN) based error correction method for EDPs obtained by the ordinary Abel inversion. The ANN is first trained to learn the relationship between vertical total electron content (TEC) measurements and retrieval errors at the F2 peak, 220 km and 110 km altitudes; correction coefficients are then estimated to correct the retrieved EDPs at these three altitudes. Experiments using the NeQuick2 model and real FORMOSAT-3/COSMIC RO geometry show that the proposed method outperforms existing ones. Real incoherent scatter radar (ISR) measurements at the Jicamarca Radio Observatory and the global TEC map provided by the International GNSS Service (IGS) are also used to valid the proposed method.

  6. Time-Quality Tradeoff of Waiting Strategies for Tutors to Retrieve Relevant Teaching Methods

    ERIC Educational Resources Information Center

    Shih, Wen-Chung; Tseng, Shian-Shyong; Yang, Che-Ching; Liang, Tyne

    2011-01-01

    As more and more undergraduate students act as voluntary tutors to rural pupils after school, there is a growing need for a supporting environment to facilitate adaptive instruction. Among others, a teaching method retrieval system is intended to help tutors find relevant teaching methods for teaching a particular concept. However, teaching…

  7. An Information Retrieval Model Based on Vector Space Method by Supervised Learning.

    ERIC Educational Resources Information Center

    Tai, Xiaoying; Ren, Fuji; Kita, Kenji

    2002-01-01

    Proposes a method to improve retrieval performance of the vector space model by using users' relevance feedback. Discusses the use of singular value decomposition and the latent semantic indexing model, and reports the results of two experiments that show the effectiveness of the proposed method. (Author/LRW)

  8. [A new retrieval method for ozone concentration at the troposphere based on differential absorption lidar].

    PubMed

    Fan, Guang-Qiang; Liu, Jian-Guo; Liu, Wen-Qing; Lu, Yi-Huai; Zhang, Tian-Shu; Dong, Yun-Sheng; Zhao, Xue-Song

    2012-12-01

    Aerosols interfere with differential absorption lidar ozone concentration measurement and can introduce significant errors. A new retrieval method was introduced, and ozone concentration and aerosol extinction coefficient were gained simultaneously based on the retrieval method. The variables were analyzed by experiment including aerosol lidar ratio, aerosol wavelength exponent, and aerosol-molecular ratio at the reference point. The results show that these parameters introduce error less than 8% below 1 km. The measurement error derives chiefly from signal noise and the parameters introduce error less than 3% above 1 km. Finally the vertical profile of tropospheric ozone concentration and aerosol extinction coefficient were derived by using this algorithm. The retrieval results of the algorithm and traditional dual-wavelength difference algorithm are compared and analyzed. Experimental results indicate that the algorithm is feasible, and the algorithm can reduce differential absorption lidar measurement error introduced by aerosol.

  9. Retrieval of Temperature and Water Vapour from Multiple Channel Lidar Systems Using an Optimal Estimation Method

    NASA Astrophysics Data System (ADS)

    Sica, Robert; Haefele, Alexander

    2016-04-01

    While the application of optimal estimation methods (OEMs) is well-known for the retrieval of atmospheric parameters from passive instruments, active instruments have typically not employed the OEM. For instance, the measurement of temperature in the middle atmosphere with Rayleigh-scatter lidars is an important technique for assessing atmospheric change. Current retrieval schemes for these temperatures have several shortcomings which can be overcome using an OEM. Forward models have been constructed that fully characterize the measurement and allow the simultaneous retrieval of temperature, dead time and background. The OEM allows a full uncertainty budget to be obtained on a per profile basis that includes, in addition to the statistical uncertainties, the smoothing error and uncertainties due to Rayleigh extinction, ozone absorption, the lidar constant, nonlinearity in the counting system, variation of the Rayleigh-scatter cross section with altitude, pressure, acceleration due to gravity and the variation of mean molecular mass with altitude. The vertical resolution of the temperature profile is found at each height, and a quantitative determination is made of the maximum height to which the retrieval is valid. A single temperature profile can be retrieved from measurements with multiple channels that cover different height ranges, vertical resolutions and even different detection methods. The OEM employed is shown to give robust estimates of temperature consistent with previous methods, while requiring minimal computational time. Retrieval of water vapour mixing ratio from vibrational Raman scattering lidar measurements is another example where an OEM offers a considerable advantage over the standard analysis technique, with the same advantages as discussed above for Rayleigh-scatter temperatures but with an additional benefit. The conversion of the lidar measurement into mixing ratio requires a calibration constant to be employed. Using OEM the calibration

  10. Retrieval of Temperature and Water Vapour From Multiple Channel Lidar Systems Using an Optimal Estimation Method

    NASA Astrophysics Data System (ADS)

    Sica, Robert; Haefele, Alexander

    2015-04-01

    While the application of optimal estimation methods (OEMs) is well-known for the retrieval of atmospheric parameters from passive instruments, active instruments have typically not employed the OEM. For instance, the measurement of temperature in the middle atmosphere with Rayleigh-scatter lidars is an important technique for assessing atmospheric change. Current retrieval schemes for these temperatures have several shortcomings which can be overcome using an OEM. Forward models have been constructed that fully characterize the measurement and allow the simultaneous retrieval of temperature, dead time and background. The OEM allows a full uncertainty budget to be obtained on a per profile basis that includes, in addition to the statistical uncertainties, the smoothing error and uncertainties due to Rayleigh extinction, ozone absorption, the lidar constant, nonlinearity in the counting system, variation of the Rayleigh-scatter cross section with altitude, pressure, acceleration due to gravity and the variation of mean molecular mass with altitude. The vertical resolution of the temperature profile is found at each height, and a quantitative determination is made of the maximum height to which the retrieval is valid. A single temperature profile can be retrieved from measurements with multiple channels that cover different height ranges, vertical resolutions and even different detection methods. The OEM employed is shown to give robust estimates of temperature consistent with previous methods, while requiring minimal computational time. Retrieval of water vapour mixing ratio from vibrational Raman scattering lidar measurements is another example where an OEM offers a considerable advantage over the standard analysis technique, with the same advantages as discussed above for Rayleigh-scatter temperatures but with an additional benefit. The conversion of the lidar measurement into mixing ratio requires a calibration constant to be employed. Using OEM the calibration

  11. Noninvasive method for retrieval of broken dental implant abutment screw

    PubMed Central

    Gooty, Jagadish Reddy; Palakuru, Sunil Kumar; Guntakalla, Vikram Reddy; Nera, Mahipal

    2014-01-01

    Dental implants made of titanium for replacement of missing teeth are widely used because of ease of technical procedure and high success rate, but are not free of complications and may fail. Fracturing of the prosthetic screw continues to be a problem in restorative practice and great challenge to remove the fractured screw conservatively. This case report describes and demonstrates the technique of using an ultrasonic scaler in the removal of the fracture screw fragment as a noninvasive method without damaging the hex of implants. PMID:24963261

  12. High sensitivity phase retrieval method in grating-based x-ray phase contrast imaging

    SciTech Connect

    Wu, Zhao; Gao, Kun; Chen, Jian; Wang, Dajiang; Wang, Shenghao; Chen, Heng; Bao, Yuan; Shao, Qigang; Wang, Zhili; Zhang, Kai; Zhu, Peiping; Wu, Ziyu

    2015-02-15

    Purpose: Grating-based x-ray phase contrast imaging is considered as one of the most promising techniques for future medical imaging. Many different methods have been developed to retrieve phase signal, among which the phase stepping (PS) method is widely used. However, further practical implementations are hindered, due to its complex scanning mode and high radiation dose. In contrast, the reverse projection (RP) method is a novel fast and low dose extraction approach. In this contribution, the authors present a quantitative analysis of the noise properties of the refraction signals retrieved by the two methods and compare their sensitivities. Methods: Using the error propagation formula, the authors analyze theoretically the signal-to-noise ratios (SNRs) of the refraction images retrieved by the two methods. Then, the sensitivities of the two extraction methods are compared under an identical exposure dose. Numerical experiments are performed to validate the theoretical results and provide some quantitative insight. Results: The SNRs of the two methods are both dependent on the system parameters, but in different ways. Comparison between their sensitivities reveals that for the refraction signal, the RP method possesses a higher sensitivity, especially in the case of high visibility and/or at the edge of the object. Conclusions: Compared with the PS method, the RP method has a superior sensitivity and provides refraction images with a higher SNR. Therefore, one can obtain highly sensitive refraction images in grating-based phase contrast imaging. This is very important for future preclinical and clinical implementations.

  13. A Novel Method of Case Representation and Retrieval in CBR for E-Learning

    ERIC Educational Resources Information Center

    Khamparia, Aditya; Pandey, Babita

    2017-01-01

    In this paper we have discussed a novel method which has been developed for representation and retrieval of cases in case based reasoning (CBR) as a part of e-learning system which is based on various student features. In this approach we have integrated Artificial Neural Network (ANN) with Data mining (DM) and CBR. ANN is used to find the…

  14. Clustering Methods; Part IV of Scientific Report No. ISR-18, Information Storage and Retrieval...

    ERIC Educational Resources Information Center

    Cornell Univ., Ithaca, NY. Dept. of Computer Science.

    Two papers are included as Part Four of this report on Salton's Magical Automatic Retriever of Texts (SMART) project report. The first paper: "A Controlled Single Pass Classification Algorithm with Application to Multilevel Clustering" by D. B. Johnson and J. M. Laferente presents a single pass clustering method which compares favorably…

  15. A Proposed Method of Measuring the Utility of Individual Information Retrieval Tools.

    ERIC Educational Resources Information Center

    Meadow, Charles T.

    1996-01-01

    Proposes a method of evaluating information retrieval systems by concentrating on individual tools (commands, their menus or graphic interface equivalents, or a move/stratagem). A user would assess the relative success of a small part of a search, and every tool used in that part would be credited with a contribution to the result. Cumulative…

  16. Development of the CODER System: A Testbed for Artificial Intelligence Methods in Information Retrieval.

    ERIC Educational Resources Information Center

    Fox, Edward A.

    1987-01-01

    Discusses the CODER system, which was developed to investigate the application of artificial intelligence methods to increase the effectiveness of information retrieval systems, particularly those involving heterogeneous documents. Highlights include the use of PROLOG programing, blackboard-based designs, knowledge engineering, lexicological…

  17. Wave propagation retrieval method for metamaterials: Unambiguous restoration of effective parameters

    NASA Astrophysics Data System (ADS)

    Andryieuski, Andrei; Malureanu, Radu; Lavrinenko, Andrei V.

    2009-11-01

    In this Brief report we propose a direct method of effective-parameters restoration that is based on the wave propagation phenomenon. It is easy in implementation, has no unambiguity in retrieving effective properties and is applicable to thick metamaterial (MTM) slabs. The method is validated on the case studies of fishnet, split cube in carcass, and Jerusalem cross MTMs. The constraints of the method are designated.

  18. A Method of Protein Model Classification and Retrieval Using Bag-of-Visual-Features

    PubMed Central

    Ma, Ziping; Kang, Baosheng; Lu, Ke

    2014-01-01

    In this paper we propose a novel visual method for protein model classification and retrieval. Different from the conventional methods, the key idea of the proposed method is to extract image features of proteins and measure the visual similarity between proteins. Firstly, the multiview images are captured by vertices and planes of a given octahedron surrounding the protein. Secondly, the local features are extracted from each image of the different views by the SURF algorithm and are vector quantized into visual words using a visual codebook. Finally, KLD is employed to calculate the similarity distance between two feature vectors. Experimental results show that the proposed method has encouraging performances for protein retrieval and categorization as shown in the comparison with other methods. PMID:25258644

  19. Assessing the impact of different satellite retrieval methods on forecast available potential energy

    NASA Technical Reports Server (NTRS)

    Whittaker, Linda M.; Horn, Lyle H.

    1990-01-01

    The effects of the inclusion of satellite temperature retrieval data, and of different satellite retrieval methods, on forecasts made with the NASA Goddard Laboratory for Atmospheres (GLA) fourth-order model were investigated using, as the parameter, the available potential energy (APE) in its isentropic form. Calculation of the APE were used to study the differences in the forecast sets both globally and in the Northern Hemisphere during 72-h forecast period. The analysis data sets used for the forecasts included one containing the NESDIS TIROS-N retrievals, the GLA retrievals using the physical inversion method, and a third, which did not contain satellite data, used as a control; two data sets, with and without satellite data, were used for verification. For all three data sets, the Northern Hemisphere values for the total APE showed an increase throughout the forecast period, mostly due to an increase in the zonal component, in contrast to the verification sets, which showed a steady level of total APE.

  20. Application of information-retrieval methods to the classification of physical data

    NASA Technical Reports Server (NTRS)

    Mamotko, Z. N.; Khorolskaya, S. K.; Shatrovskiy, L. I.

    1975-01-01

    Scientific data received from satellites are characterized as a multi-dimensional time series, whose terms are vector functions of a vector of measurement conditions. Information retrieval methods are used to construct lower dimensional samples on the basis of the condition vector, in order to obtain these data and to construct partial relations. The methods are applied to the joint Soviet-French Arkad project.

  1. Fresnel phase retrieval method using an annular lens array on an SLM

    NASA Astrophysics Data System (ADS)

    Loriot, V.; Mendoza-Yero, O.; Pérez-Vizcaíno, J.; Mínguez-Vega, G.; de Nalda, R.; Bañares, L.; Lancis, J.

    2014-10-01

    Wavefront aberrations play a major role when focusing an ultrashort laser pulse to a high-quality focal spot. Here, we report a novel method to measure and correct wavefront aberrations of a 30-fs pulsed laser beam. The method only requires a programmable liquid-crystal spatial light modulator and a camera. Wavefront retrieval is based on pupil segmentation with an annular lens array, which allows us to determine the local phase that minimizes focusing errors due to wavefront aberrations. Our method provides accurate results even when implemented with low dynamic range cameras and polychromatic beams. Finally, the retrieved phase is added to a diffractive lens codified onto the spatial light modulator to experimentally demonstrate near-diffraction-limited femtosecond beam focusing without refractive components.

  2. Retrieval of Aerosol information from UV measurement by using optimal estimation method

    NASA Astrophysics Data System (ADS)

    KIM, M.; Kim, J.; Jeong, U.; Kim, W. V.; Kim, S. K.; Lee, S. D.; Moon, K. J.

    2014-12-01

    An algorithm to retrieve aerosol optical depth (AOD), single scattering albedo (SSA), and aerosol loading height is developed for GEMS (Geostationary Environment Monitoring Spectrometer) measurement. The GEMS is planned to be launched in geostationary orbit in 2018, and employs hyper-spectral imaging with 0.6 nm resolution to observe solar backscatter radiation in the UV and Visible range. In the UV range, the low surface contribution to the backscattered radiation and strong interaction between aerosol absorption and molecular scattering can be advantageous in retrieving aerosol information such as AOD and SSA [Torres et al., 2007; Torres et al., 2013; Ahn et al., 2014]. However, the large contribution of atmospheric scattering results in the increase of the sensitivity of the backward radiance to aerosol loading height. Thus, the assumption of aerosol loading height becomes important issue to obtain accurate result. Accordingly, this study focused on the simultaneous retrieval of aerosol loading height with AOD and SSA by utilizing the optimal estimation method. For the RTM simulation, the aerosol optical properties were analyzed from AERONET inversion data (level 2.0) at 46 AERONET sites over ASIA. Also, 2-channel inversion method is applied to estimate a priori value of the aerosol information to solve the Lavenberg Marquardt equation. The GEMS aerosol algorithm is tested with OMI level-1B dataset, a provisional data for GEMS measurement, and the result is compared with OMI standard aerosol product and AERONET values. The retrieved AOD and SSA show reasonable distribution compared with OMI products, and are well correlated with the value measured from AERONET. However, retrieval uncertainty in aerosol loading height is relatively larger than other results.

  3. Method of content-based image retrieval for a spinal x-ray image database

    NASA Astrophysics Data System (ADS)

    Krainak, Daniel M.; Long, L. Rodney; Thoma, George R.

    2002-05-01

    The Lister Hill National Center for Biomedical Communications, a research and development division of the National Library of Medicine (NLM) maintains a digital archive of 17,000 cervical and lumbar spine images collected in the second National Health and Nutrition Examination Survey (NHANES II) conducted by the National Center for Health Statistics (NCHS). Classification of the images for the osteoarthritis research community has been a long-standing goal of researchers at the NLM, collaborators at NCHS, and the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), and capability to retrieve images based on geometric characteristics of the vertebral bodies is of interest to the vertebral morphometry community. Automated or computer-assisted classification and retrieval methods are highly desirable to offset the high cost of manual classification and manipulation by medical experts. We implemented a prototype system for a database of 118 spine x-rays and health survey text data related to these x-rays. The system supports conventional text retrieval, as well as retrieval based on shape similarity to a user-supplied vertebral image or sketch.

  4. Retrieval of particle size distribution in the dependent model using the moment method.

    PubMed

    Sun, Xiaogang; Tang, Hong; Dai, Jingmin

    2007-09-03

    The problem of determining particle size distribution using the moment method in the spectral extinction technique is studied. The feasibility and reliability of the retrieval of spherical particle size distribution using the moment method are investigated. The single spherical particle extinction efficiency, which is derived theoretically using the Mie's solution to Maxwell's equation, is approximated with a higher order polynomial in order to apply the moment method. Simulation and experimental results indicate that a fairly reasonable representation of the particle size distribution can be obtained using the moment method in the dependent model algorithm. The method has advantages of simplicity, rapidity, and suitability for in-line particle size measurement.

  5. [Concentration retrieving method of SO2 using differential optical absorption spectroscopy based on statistics].

    PubMed

    Liu, Bin; Sun, Chang-Ku; Zhang, Chi; Zhao, Yu-Mei; Liu, Jun-Ping

    2011-01-01

    A concentration retrieving method using statistics is presented, which is applied in differential optical absorption spectroscopy (DOAS) for measuring the concentration of SO2. The method uses the standard deviation of the differential absorption to represents the gas concentration. Principle component analysis (PCA) method is used to process the differential absorption spectrum. In the method, the basis data for the concentration retrieval of SO2 is the combination of the PCA processing result, the correlation coefficient, and the standard deviation of the differential absorption. The method is applied to a continuous emission monitoring system (CEMS) with optical path length of 0.3 m. Its measuring range for SO2 concentration is 0-5 800 mg x m(-3). The nonlinear calibration and the temperature compensation for the system were executed. The full scale error of the retrieving concentration is less than 0.7% FS. And the measuring result is -4.54 mg x m(-3) when the concentration of SO2 is zero.

  6. [A new method for detection and erradication of Helicobacter pylori infection by stool antigens test].

    PubMed

    Amèndola, R; Doweck, J; Katz, J; Racca, J; Menendez, G; Schenone, L; Farìas, R; Barrantes, C; Quintanta, C; Zerbo, O; Kogan, Z; Valero, J; Bartellini, M A; Questa, U; Luna, P; Corti, R E

    2002-01-01

    Nowadays technics for Helicobacter pylori detection in stools like culture, and PCR, are expensive and difficult to perform. The aim of this study was to evaluate ELISA test efficacy for detection of H. Pylori antigens in stools comparing this results with standarized technics like histology (Giemsa), ureasa test and UBT C 14. 26 patients were evaluated in this study, ages between 15-75 with upper gastrointestinal symptoms; all of them required gastroduodenal endoscopy, status H. Pylori was determined with methods upon mentioned. 24 hours after endoscopy H. Pylori antigens in stools with the technique Premier Platinum Htsa, Elisa were determined. The detection of H. Pylori antigens in stools accurately identified active H. Pylori infection. The performance characteristics of this non-invasive method was similar in sensibility and specificity to conventional tests.

  7. A comparison of three retrieval methods with single Doppler radar data

    NASA Astrophysics Data System (ADS)

    Zhou, Shenghui; Wei, Ming; Gao, Li; Wang, Hao; Zeng, Qingfeng; Yang, Kun

    2011-11-01

    In single Doppler radar wind retrieval technique there are three methods proposed in recently years, which is VAP, VPP and SVVP method. Three methods were employed to retrieve wind field with the purpose to find out which one is more suitable in practice according their accuracy by using synthetic data and real data. When tests applied with uniform data, the orders of magnitude for relative error of radial velocity is 10-13 for SVVP, much lower than that of VAP and VPP which both are 10-4. Furthermore, the results under condition of wind direction unchanged and wind velocity varied linearity with altitude are 10-8, 10-3, 10-3 for SVVP, VPP and VAP, respectively. In real wind field of typhoon "Pearl", the authenticity of surrounding wind and wind shear retrieved by SVVP and VPP is better than VAP, the deviation of simulation would be increased at some points missing data. Relative errors of radial velocity achieved by VAP, VPP and SVVP are 7.02, 5.78 and 3.1 respectively. It indicated that SVVP method's performance is better than that of other two and suitable in practical applications.

  8. A coordinated retrieval method for sea surface salinity based on SMOS and ocean color data

    NASA Astrophysics Data System (ADS)

    Chen, Peng; Wang, Tianyu; Mao, Zhihua; Bai, Yan; Hao, Zengzhou

    2016-10-01

    A coordinated retrieval method for sea surface salinity based on SMOS and ocean color data was developed. The method retrieved sea surface salinity in open sea based on SMOS data, and those with much RFIs in the coastal area using ocean color data, aCDOM. Tight relationships between surface water salinity and in situ aCDOM were found during the cruises in the Yangtze River estuary on April 2013 and Hangzhou Bay in May 2014, distributions of aCDOM revealed gradual downward trends of magnitudes, as water flowed down the Yangtze River estuary into the ECS coast. A dilution process was detected as water flowed down the Yangtze River and into the ECS coast. Thus, a salinity inversion model from the negative relationship between salinity and aCDOM was developed firstly. Then we matched the SSS products with different spatial resolution retrieved based on SMOS and ocean color and combined them. In the end, we compared the SSS measurements between those based on only SMOS data and those based on method in this paper, and found that the method can make up the phenomenon of lack of data effectively.

  9. The Influence of Learning Methods on Collaboration: Prior Repeated Retrieval Enhances Retrieval Organization, Abolishes Collaborative Inhibition, and Promotes Post-Collaborative Memory

    ERIC Educational Resources Information Center

    Congleton, Adam R.; Rajaram, Suparna

    2011-01-01

    Research on collaborative memory has unveiled the counterintuitive yet robust phenomenon that collaboration impairs group recall. A candidate explanation for this "collaborative inhibition" effect is the disruption of people's idiosyncratic retrieval strategies during collaboration, and it is hypothesized that employing methods that improve one's…

  10. System and method for phase retrieval for radio telescope and antenna control

    NASA Technical Reports Server (NTRS)

    Dean, Bruce H. (Inventor)

    2013-01-01

    Disclosed herein are systems, methods, and non-transitory computer-readable storage media for radio phase retrieval. A system practicing the method gathers first data from radio waves associated with an object observed via a first aperture, gathers second data from radio waves associated with the object observed via an introduced second aperture associated with the first aperture, generates reduced noise data by incoherently subtracting the second data from the first data, and performs phase retrieval for the radio waves by modeling the reduced noise data using a single Fourier transform. The first and second apertures are at different positions, such as side by side. This approach can include determining a value Q which represents a ratio of wavelength times a focal ratio divided by pixel spacing. This information can be used to accurately measure and correct alignment errors or other optical system flaws in the apertures.

  11. Ptychographic phase retrieval method for characterizing ultra-precise ellipsoidal mirrors

    NASA Astrophysics Data System (ADS)

    Takeo, Yoko; Saito, Takahiro; Mimura, Hidekazu

    2015-10-01

    Focusing and imaging optics can be characterized by evaluating the wavefront error of the focused beam. We have bean developing a ptychographic phase retrieval method using a visible laser to measure the wavefront error. In this study, the measurement accuracy of the method is increased by improving both the phase retrieval algorithm and the experimental setup. The system is applied to the characterization of an ellipsoidal mirror used for the focusing of soft X-rays. The posture of the mirror can be measured with a resolution of 1.4 μrad. The wavefront error originating from the surface profile error can be detected with an accuracy of 0.01λ (root mean square).

  12. Vibration-resistant phase retrieval method with contrast compensation for phase-shifting interferometry

    NASA Astrophysics Data System (ADS)

    Liu, Qian; He, Huabin; Yuan, Daocheng; He, Jianguo; Ji, Fang

    2017-04-01

    Vibration hinders the application of phase-shifting interferometry (PSI) to on-machine test and large-aperture mirror measurement. The investigation of PSI fringe disturbed by vibration indicates that, besides tilt-shifting error, inter- and intra-frame contrast variation is significant. The contrast variation is another dominant error source in phase retrieval of PSI. An inter- and intra-frame contrast compensation method is proposed here to retrieve wavefront phase from interferograms subjected to vibration. The method constructs algebraic equations with interferogram data and solves equations using iterative procedures. Experiments validate its effectiveness and manifest its capability to suppress vibration-induced error over a large frequency region. To enhance the calculation efficiency, a spatial subsampling strategy is proposed. Practical testing shows that subsampling reduces calculation time exponentially and preserves retrieval accuracy and spatial resolution. The proposed method, of which the unique ability is compensating the tilt-shifting error and fringe blur caused by vibration, predicates an effective and low-cost solution for PSI applied in vibration.

  13. Distributed data organization and parallel data retrieval methods for huge laser scanner point clouds

    NASA Astrophysics Data System (ADS)

    Hongchao, Ma; Wang, Zongyue

    2011-02-01

    This paper proposes a novel method for distributed data organization and parallel data retrieval from huge volume point clouds generated by airborne Light Detection and Ranging (LiDAR) technology under a cluster computing environment, in order to allow fast analysis, processing, and visualization of the point clouds within a given area. The proposed method is suitable for both grid and quadtree data structures. As for distribution strategy, cross distribution of the dataset would be more efficient than serial distribution in terms of non-redundant datasets, since a dataset is more uniformly distributed in the former arrangement. However, redundant datasets are necessary in order to meet the frequent need of input and output operations in multi-client scenarios: the first copy would be distributed by a cross distribution strategy while the second (and later) would be distributed by an iterated exchanging distribution strategy. Such a distribution strategy would distribute datasets more uniformly to each data server. In data retrieval, a greedy algorithm is used to allocate the query task to a data server, where the computing load is lightest if the data block needing to be retrieved is stored among multiple data servers. Experiments show that the method proposed in this paper can satisfy the demands of frequent and fast data query.

  14. Development and application of new methods to retrieve vertical structure of precipitation above the ARM CART sites from MMCR measurements

    SciTech Connect

    Matrosov, Sergey

    2010-12-15

    The main objective of this project was to develop, validate and apply remote sensing methods to retrieve vertical profiles of precipitation over the DOE ARM CART sites using currently available remote sensors. While the ARM Program invested very heavily into developments of remote sensing methods and instruments for water vapor and non-precipitating cloud parameter retrievals, precipitation retrievals and studies lagged behind. Precipitation, however, is a crucial part of the water cycle, and without detailed information on rainfall and snowfall, significant improvements in the atmospheric models of different scales (i.e., one of the ARM Program's main goals) is difficult to achieve. Characterization of the vertical atmospheric column above the CART sites is also incomplete without detailed precipitation information, so developments of remote sensing methods for retrievals of parameters in precipitating cloud condition was essential. Providing modelers with retrieval results was also one of the key objectives of this research project.

  15. Modeling formalin fixation and antigen retrieval with bovine pancreatic ribonuclease A: I—Structural and functional alterations

    PubMed Central

    Rait, Vladimir K; O’Leary, Timothy J; Mason, Jeffrey T

    2006-01-01

    Understanding the chemistry of protein modification by formaldehyde is central to developing improved methods to recover proteins from formalin-fixed paraffin-embedded tissues for proteomic analysis and to improve protein immunoreactivity for immunohistochemical studies. We used biophysical techniques to investigate the effects of formaldehyde treatment on bovine pancreatic ribonuclease A (RNase A). Treatment of RNase A with formaldehyde was shown by gel electrophoresis to lead to the rapid formation of intra- and intermolecular protein cross-links. Thermal studies revealed that these protein cross-links significantly increased the thermal denaturation temperature of RNase A preparations. Analysis of formaldehyde-treated RNase A oligomers isolated by gel chromatography revealed that intramolecular protein cross-links are primarily responsible for the increase in protein thermostability. Formaldehyde treatment also lowered the isoelectric point of the enzyme from 9.45 to the 6.0–7.4 range. Optical spectroscopic studies demonstrated that the formaldehyde-induced modifications did not significantly alter the secondary or tertiary structure of RNase A. Heating formaldehyde-treated RNase A at 65°C resulted in a significant reversal of the protein intra- and intermolecular cross-links and led to a partial restoration of enzymatic activity. PMID:14968117

  16. Evaluation of four methods for the detection of streptococcal group A antigen directly from throat swabs.

    PubMed

    Betriu, C; de la Torre, F; Muñoz, P; Fernández, A; Picazo, J J

    1988-12-01

    We have compared the sensitivity, specificity and reproducibility of four rapid tests for the detection of group A beta-hemolytic streptococci antigen directly from a throat swab. The four methods were very specific, all of them offered reproductibility and surpassed conventional culture in speed and simplicity.

  17. A comparison of dimensionality reduction methods for retrieval of similar objects in simulation data

    SciTech Connect

    Cantu-Paz, E; Cheung, S S; Kamath, C

    2003-09-23

    High-resolution computer simulations produce large volumes of data. As a first step in the analysis of these data, supervised machine learning techniques can be used to retrieve objects similar to a query that the user finds interesting. These objects may be characterized by a large number of features, some of which may be redundant or irrelevant to the similarity retrieval problem. This paper presents a comparison of six dimensionality reduction algorithms on data from a fluid mixing simulation. The objective is to identify methods that efficiently find feature subsets that result in high accuracy rates. Our experimental results with single- and multi-resolution data suggest that standard forward feature selection produces the smallest feature subsets in the shortest time.

  18. A physical multispectral method for the retrieval of ocean and lake surface temperatures via scanning spectrometer

    NASA Astrophysics Data System (ADS)

    Nalli, Nicholas Rocco

    Accurate measurements of water surface skin temperatures (WST) from satellite are required in numerous research and civil applications. Of particular importance are climate studies which require a high degree of absolute accuracy (≤0.3 K). In this work a physical retrieval methodology is developed for general application to any scanning spectrometer that obtains multispectral IR window radiance measurements. Such instrumentation includes high resolution Fourier transform spectrometer (FTS) systems as well as narrowband channel radiometers. To achieve this a rough surface reflection model is developed to be used in tandem with an emissivity model previously developed by Wu and Smith [1997]. The model assumes the Kirchhoff approximation (valid for IR wavelengths and water surfaces) for rough surface scattering. The wave slope statistics of Cox and Munk [1954, 1955] are then employed for the double integration of reflected rays over zenith and azimuth angle. The otherwise involved computation is performed via Gaussian quadrature using an uplooking fast transmittance model. The full hemispherical model calculation is simplified by the novel concept of a reflection-diffusivity angle. This parameter is a function of wavenumber, viewing angle, wind speed and total uplooking transmittance. A lookup table is generated for convenient application in WST retrieval. The model compares favorably against observed radiance spectra obtained from the Marine Atmospheric Emitted Radiance Interferometer (M-AERI) in the tropical Pacific ocean during the Combined Sensor Program (CSP) research cruise. The improved WST retrieval method which utilizes the reflection model exhibits less scatter and moisture-dependent bias in simulation. The method is applied to microwindow radiance spectra measured by the NPOESS Airborne Sounder Testbed-Interferometer (NAST-I) from the NASA ER-2 during two field campaigns. High resolution lake and sea surface temperature fields are measured which reveal

  19. A spectral method for retrieving cloud optical thickness and effective radius from surface-based transmittance measurements

    NASA Astrophysics Data System (ADS)

    McBride, P. J.; Schmidt, K. S.; Pilewskie, P.; Kittelman, A. S.; Wolfe, D. E.

    2011-07-01

    We introduce a new spectral method for the retrieval of optical thickness and effective radius from cloud transmittance that relies on the spectral slope of the normalized transmittance between 1565 nm and 1634 nm, and on cloud transmittance at a visible wavelength. The standard dual-wavelength technique, which is traditionally used in reflectance-based retrievals, is ill-suited for transmittance because it lacks sensitivity to effective radius, especially for optically thin clouds. Using the spectral slope rather than the transmittance itself enhances the sensitivity of transmittance observations with respect to the effective radius. This is demonstrated by applying it to the moderate spectral resolution observations from the Solar Spectral Flux Radiometer (SSFR) and Shortwave Spectroradiometer (SWS), and by examining the retrieval uncertainties of the standard and the spectral method for data from the DOE ARM Southern Great Plains (SGP) site and a NOAA ship cruise (ICEALOT). The liquid water path (LWP) is derived from the retrieved optical thickness and effective radius, based on two different assumptions about the cloud vertical profile, and compared to the simultaneous observations from a microwave radiometer. Optical thickness and effective radius is also compared to MODIS retrievals. In general, the effective radius uncertainties were much larger for the standard retrieval than for the spectral retrieval, particularly for thin clouds. When defining 2 μm as upper limit for the tolerable uncertainty of the effective radius, the standard method returned only very few valid retrievals for clouds with an optical thickness below 25. For the analyzed ICEALOT data (mean optical thickness 23), the spectral method provided valid retrievals for 84 % of the data (24 % for the standard method). For the SGP data (mean optical thickness 44), both methods provided a high return of 90 % for the spectral method and 78 % for the standard method.

  20. Skill in Retrievals

    NASA Technical Reports Server (NTRS)

    Aumann, Hartmut H.; Manning, Evan

    2008-01-01

    Retrieval Skill quantifies the ability of one retrieval from a sounder to be more accurate than the best forecast relative to another with the same of another sounder. This is summarized using a Retrieval Anomaly Skill Score (RASS) which is the cor (retrieved-background, truth-background) * sqrt(f), Where f is defined as the ratio of accepted to the possible retrievals. Charts show various features and comparisons of RASS to other methods of retrieval.

  1. Physical retrieval of precipitation water contents from Special Sensor Microwave/Imager (SSM/I) data. Part 2: Retrieval method and applications (report version)

    NASA Technical Reports Server (NTRS)

    Olson, William S.

    1990-01-01

    A physical retrieval method for estimating precipitating water distributions and other geophysical parameters based upon measurements from the DMSP-F8 SSM/I is developed. Three unique features of the retrieval method are (1) sensor antenna patterns are explicitly included to accommodate varying channel resolution; (2) precipitation-brightness temperature relationships are quantified using the cloud ensemble/radiative parameterization; and (3) spatial constraints are imposed for certain background parameters, such as humidity, which vary more slowly in the horizontal than the cloud and precipitation water contents. The general framework of the method will facilitate the incorporation of measurements from the SSMJT, SSM/T-2 and geostationary infrared measurements, as well as information from conventional sources (e.g., radiosondes) or numerical forecast model fields.

  2. Note: Phase retrieval method for analyzing single-phase displacement interferometry data

    SciTech Connect

    Chen, X. H.; Zeng, X. L.; Fan, D.; Liu, Q. C.; Bie, B. X.; Zhou, X. M. Luo, S. N.

    2014-02-15

    We present a phase retrieval method (PRM) for analyzing single-phase displacement interferometry measurements on rapidly changing velocity histories, including photon Doppler velocimetry (PDV). PRM identifies the peaks and valleys as well as zero-crossing points in a PDV time series, performs normalization and extracts point-by-point phase and thus velocity information. PRM does not require a wide time window as in sliding window Fourier transformation, and thus improves the effective temporal resolution. This method is implemented in analyzing PDV data obtained from gas gun experiments, and validated against simultaneous measurements with velocity interferometer system for any reflector.

  3. Note: Phase retrieval method for analyzing single-phase displacement interferometry data

    NASA Astrophysics Data System (ADS)

    Chen, X. H.; Zeng, X. L.; Fan, D.; Liu, Q. C.; Bie, B. X.; Zhou, X. M.; Luo, S. N.

    2014-02-01

    We present a phase retrieval method (PRM) for analyzing single-phase displacement interferometry measurements on rapidly changing velocity histories, including photon Doppler velocimetry (PDV). PRM identifies the peaks and valleys as well as zero-crossing points in a PDV time series, performs normalization and extracts point-by-point phase and thus velocity information. PRM does not require a wide time window as in sliding window Fourier transformation, and thus improves the effective temporal resolution. This method is implemented in analyzing PDV data obtained from gas gun experiments, and validated against simultaneous measurements with velocity interferometer system for any reflector.

  4. Indirect methods of retrieval of important mesospheric gas constituents from available measurements data

    NASA Astrophysics Data System (ADS)

    Kulikov, Mikhail; Nechaev, Anton; Belikovich, Mikhail; Ermakova, Tatiana; Feigin, Alexander

    2016-04-01

    Observation of minor gas constituents is traditionally considered as one of the fundamental problems in investigations of the Earth's mesosphere and mesopause region. Since direct and regular measurements are still rather scanty, indirect methods are a useful tool. Based on a priori assumptions about possible relationships between measured and retrieved characteristics (for example, photochemical models), they allow one to obtain additional information about the important constituents from available experimental data. In this presentation we discuss, firstly, the restrictions on the use of the ozone steady state equilibrium condition for the retrieval of atomic oxygen and hydrogen concentrations from O2 and OH airglow emissions ratios and O3 concentration measurements. Secondly, we present a statistical Bayesian procedure of retrieving the concentrations of important constituents in mesosphere from available satellite and ground based experimental data. The technique is based on the application of the so-called basic dynamic model of mesospheric photochemistry, which is simplified mathematically correctly in a special manner and describes the most significant reactions between components of the families of odd hydrogen and oxygen. The work was supported by the Russian Science Foundation (Contract No. 15-17-10024).

  5. Developments of global greenhouse gas retrieval algorithm based on Optimal Estimation Method

    NASA Astrophysics Data System (ADS)

    Kim, W. V.; Kim, J.; Lee, H.; Jung, Y.; Boesch, H.

    2013-12-01

    After the industrial revolution, atmospheric carbon dioxide concentration increased drastically over the last 250 years. It is still increasing and over than 400ppm of carbon dioxide was measured at Mauna Loa observatory for the first time which value was considered as important milestone. Therefore, understanding the source, emission, transport and sink of global carbon dioxide is unprecedentedly important. Currently, Total Carbon Column Observing Network (TCCON) is operated to observe CO2 concentration by ground base instruments. However, the number of site is very few and concentrated to Europe and North America. Remote sensing of CO2 could supplement those limitations. Greenhouse Gases Observing SATellite (GOSAT) which was launched 2009 is measuring column density of CO2 and other satellites are planned to launch in a few years. GOSAT provide valuable measurement data but its low spatial resolution and poor success rate of retrieval due to aerosol and cloud, forced the results to cover less than half of the whole globe. To improve data availability, accurate aerosol information is necessary, especially for East Asia region where the aerosol concentration is higher than other region. For the first step, we are developing CO2 retrieval algorithm based on optimal estimation method with VLIDORT the vector discrete ordinate radiative transfer model. Proto type algorithm, developed from various combinations of state vectors to find best combination of state vectors, shows appropriate result and good agreement with TCCON measurements. To reduce calculation cost low-stream interpolation is applied for model simulation and the simulation time is drastically reduced. For the further study, GOSAT CO2 retrieval algorithm will be combined with accurate GOSAT-CAI aerosol retrieval algorithm to obtain more accurate result especially for East Asia.

  6. A Novel Bayesian DNA Motif Comparison Method for Clustering and Retrieval

    PubMed Central

    Margalit, Hanah; Friedman, Nir

    2008-01-01

    Characterizing the DNA-binding specificities of transcription factors is a key problem in computational biology that has been addressed by multiple algorithms. These usually take as input sequences that are putatively bound by the same factor and output one or more DNA motifs. A common practice is to apply several such algorithms simultaneously to improve coverage at the price of redundancy. In interpreting such results, two tasks are crucial: clustering of redundant motifs, and attributing the motifs to transcription factors by retrieval of similar motifs from previously characterized motif libraries. Both tasks inherently involve motif comparison. Here we present a novel method for comparing and merging motifs, based on Bayesian probabilistic principles. This method takes into account both the similarity in positional nucleotide distributions of the two motifs and their dissimilarity to the background distribution. We demonstrate the use of the new comparison method as a basis for motif clustering and retrieval procedures, and compare it to several commonly used alternatives. Our results show that the new method outperforms other available methods in accuracy and sensitivity. We incorporated the resulting motif clustering and retrieval procedures in a large-scale automated pipeline for analyzing DNA motifs. This pipeline integrates the results of various DNA motif discovery algorithms and automatically merges redundant motifs from multiple training sets into a coherent annotated library of motifs. Application of this pipeline to recent genome-wide transcription factor location data in S. cerevisiae successfully identified DNA motifs in a manner that is as good as semi-automated analysis reported in the literature. Moreover, we show how this analysis elucidates the mechanisms of condition-specific preferences of transcription factors. PMID:18463706

  7. Pulse retrieval in frequency-resolved optical gating based on the method of generalized projections

    SciTech Connect

    DeLong, K.W.; Fittinghoff, D.N.; Trebino, R. ); Kohler, B.; Wilson, K. )

    1994-12-15

    We use the algorithmic method of generalized projections (GP's) to retrieve the intensity and phase of an ultrashort laser pulse from the experimental trace in frequency-resolved optical gating (FROG). Using simulations, we show that the use of GP's improves significantly the convergence properties of the algorithm over the basic FROG algorithm. In experimental measurements, the GP-based algorithm achieves significantly lower errors than previous algorithms. The use of GP's also permits the inclusion of an arbitrary material response function in the FROG problem.

  8. An online aerosol retrieval algorithm using OMI near-UV observations based on the optimal estimation method

    NASA Astrophysics Data System (ADS)

    Jeong, U.; Kim, J.; Ahn, C.; Torres, O.; Liu, X.; Bhartia, P. K.; Spurr, R. J. D.; Haffner, D.; Chance, K.; Holben, B. N.

    2015-06-01

    An online version of the OMI (Ozone Monitoring Instrument) near-ultraviolet (UV) aerosol retrieval algorithm was developed to retrieve aerosol optical thickness (AOT) and single scattering albedo (SSA) based on the optimal estimation (OE) method. Instead of using the traditional look-up tables for radiative transfer calculations, it performs online radiative transfer calculations with the Vector Linearized Discrete Ordinate Radiative Transfer (VLIDORT) model to eliminate interpolation errors and improve stability. The OE-based algorithm has the merit of providing useful estimates of uncertainties simultaneously with the inversion products. The measurements and inversion products of the Distributed Regional Aerosol Gridded Observation Network campaign in Northeast Asia (DRAGON NE-Asia 2012) were used to validate the retrieved AOT and SSA. The retrieved AOT and SSA at 388 nm have a correlation with the Aerosol Robotic Network (AERONET) products that is comparable to or better than the correlation with the operational product during the campaign. The estimated retrieval noise and smoothing error perform well in representing the envelope curve of actual biases of AOT at 388 nm between the retrieved AOT and AERONET measurements. The forward model parameter errors were analyzed separately for both AOT and SSA retrievals. The surface albedo at 388 nm, the imaginary part of the refractive index at 354 nm, and the number fine mode fraction (FMF) were found to be the most important parameters affecting the retrieval accuracy of AOT, while FMF was the most important parameter for the SSA retrieval. The additional information provided with the retrievals, including the estimated error and degrees of freedom, is expected to be valuable for future studies.

  9. Inverse problem of the multislice method in retrieving projected complex potentials from the exit-wave function.

    PubMed

    Lin, Fang; Jin, Chuanhong

    2014-03-01

    We proposed a new algorithm that retrieves the projected potentials from the EW of object. This algorithm is based on the traditional multislice method which involves the convolution operation in calculation. The retrieved potential is complex including both the electrostatic and absorptive components. Tests with the simulated exit waves of a 200 K InP crystal prove the algorithm effective for objects in wide thickness range. For thick specimen where dynamical electron diffraction prevails, the retrieved potential could present structure and chemical information of object by completely mapping an atom's scattering potential during interaction with incident electrons.

  10. Novel antigen identification method for discovery of protective malaria antigens by rapid testing of DNA vaccines encoding exons from the parasite genome.

    PubMed

    Haddad, Diana; Bilcikova, Erika; Witney, Adam A; Carlton, Jane M; White, Charles E; Blair, Peter L; Chattopadhyay, Rana; Russell, Joshua; Abot, Esteban; Charoenvit, Yupin; Aguiar, Joao C; Carucci, Daniel J; Weiss, Walter R

    2004-03-01

    We describe a novel approach for identifying target antigens for preerythrocytic malaria vaccines. Our strategy is to rapidly test hundreds of DNA vaccines encoding exons from the Plasmodium yoelii yoelii genomic sequence. In this antigen identification method, we measure reduction in parasite burden in the liver after sporozoite challenge in mice. Orthologs of protective P. y. yoelii genes can then be identified in the genomic databases of Plasmodium falciparum and Plasmodium vivax and investigated as candidate antigens for a human vaccine. A pilot study to develop the antigen identification method approach used 192 P. y. yoelii exons from genes expressed during the sporozoite stage of the life cycle. A total of 182 (94%) exons were successfully cloned into a DNA immunization vector with the Gateway cloning technology. To assess immunization strategies, mice were vaccinated with 19 of the new DNA plasmids in addition to the well-characterized protective plasmid encoding P. y. yoelii circumsporozoite protein. Single plasmid immunization by gene gun identified a novel vaccine target antigen which decreased liver parasite burden by 95% and which has orthologs in P. vivax and P. knowlesi but not P. falciparum. Intramuscular injection of DNA plasmids produced a different pattern of protective responses from those seen with gene gun immunization. Intramuscular immunization with plasmid pools could reduce liver parasite burden in mice despite the fact that none of the plasmids was protective when given individually. We conclude that high-throughput cloning of exons into DNA vaccines and their screening is feasible and can rapidly identify new malaria vaccine candidate antigens.

  11. A Novel Medical Freehand Sketch 3D Model Retrieval Method by Dimensionality Reduction and Feature Vector Transformation.

    PubMed

    Jing, Zhang; Sheng, Kang Bao

    2015-01-01

    To assist physicians to quickly find the required 3D model from the mass medical model, we propose a novel retrieval method, called DRFVT, which combines the characteristics of dimensionality reduction (DR) and feature vector transformation (FVT) method. The DR method reduces the dimensionality of feature vector; only the top M low frequency Discrete Fourier Transform coefficients are retained. The FVT method does the transformation of the original feature vector and generates a new feature vector to solve the problem of noise sensitivity. The experiment results demonstrate that the DRFVT method achieves more effective and efficient retrieval results than other proposed methods.

  12. A Novel Medical Freehand Sketch 3D Model Retrieval Method by Dimensionality Reduction and Feature Vector Transformation

    PubMed Central

    Jing, Zhang; Sheng, Kang Bao

    2016-01-01

    To assist physicians to quickly find the required 3D model from the mass medical model, we propose a novel retrieval method, called DRFVT, which combines the characteristics of dimensionality reduction (DR) and feature vector transformation (FVT) method. The DR method reduces the dimensionality of feature vector; only the top M low frequency Discrete Fourier Transform coefficients are retained. The FVT method does the transformation of the original feature vector and generates a new feature vector to solve the problem of noise sensitivity. The experiment results demonstrate that the DRFVT method achieves more effective and efficient retrieval results than other proposed methods. PMID:27293478

  13. List-method directed forgetting: the forget cue improves both encoding and retrieval of postcue information.

    PubMed

    Pastötter, Bernhard; Kliegl, Oliver; Bäuml, Karl-Heinz T

    2012-08-01

    In list-method directed forgetting, people are cued to forget a previously studied item list and to learn a new list instead. Such cuing typically leads to forgetting of the first list and to memory enhancement of the second, referred to as list 1 forgetting and list 2 enhancement. In the present study, two experiments are reported that examined influences of items' serial learning position in a list and the two lists' output order on list-method directed forgetting. The results show that list output order influences list 2 enhancement but not list 1 forgetting. The enhancement was higher when list 2 was recalled first than when list 1 was recalled first and, in both cases, was higher for early list 2 items than for middle and late list 2 items. In contrast, the forgetting was equally present for all list 1 items and did not depend on the two lists' output order. The findings suggest that two separate factors can contribute to list 2 enhancement: one (encoding) factor that is restricted to early list 2 items and does not depend on list output order, and another (retrieval) factor that pertains to all list 2 items and varies with the two lists' output order. A new two-mechanism account of directed forgetting is suggested that reconciles previous (encoding or retrieval) views on list 2 enhancement.

  14. Evaluation of shape indexing methods for content-based retrieval of x-ray images

    NASA Astrophysics Data System (ADS)

    Antani, Sameer; Long, L. Rodney; Thoma, George R.; Lee, Dah-Jye

    2003-01-01

    Efficient content-based image retrieval of biomedical images is a challenging problem of growing research interest. Feature representation algorithms used in indexing medical images on the pathology of interest have to address conflicting goals of reducing feature dimensionality while retaining important and often subtle biomedical features. At the Lister Hill National Center for Biomedical Communications, a R&D division of the National Library of Medicine, we are developing a content-based image retrieval system for digitized images of a collection of 17,000 cervical and lumbar x-rays taken as a part of the second National Health and Nutrition Examination Survey (NHANES II). Shape is the only feature that effectively describes various pathologies identified by medical experts as being consistently and reliably found in the image collection. In order to determine if the state of the art in shape representation methods is suitable for this application, we have evaluated representative algorithms selected from the literature. The algorithms were tested on a subset of 250 vertebral shapes. In this paper we present the requirements of an ideal algorithm, define the evaluation criteria, and present the results and our analysis of the evaluation. We observe that while the shape methods perform well on visual inspection of the overall shape boundaries, they fall short in meeting the needs of determining similarity between the vertebral shapes based on the pathology.

  15. Costs and benefits in item-method directed forgetting: differential effects of encoding and retrieval.

    PubMed

    Lee, Yuh-Shiow

    2013-01-01

    This study examined how encoding and retrieval factors affected directed forgetting costs and benefits in an item-method procedure. Experiment 1 used a typical item-method procedure and revealed a levels-of-processing effect in overall recall. However, the deep encoding condition showed a smaller directed forgetting effect than the shallow encoding conditions. More importantly, "remember" (R) words were selectively rehearsed as indicated by greater recall from the primacy portion of the list and more apt to be recalled before "forget" (F) words. Experiment 2 showed that a deep encoding operation reduced directed forgetting costs and that directed forgetting benefits occurred only when R words were recalled before F words. These findings supported the hypotheses that encoding manipulation affected directed forgetting costs and that directed forgetting benefits were associated with output order bias. Results were discussed in terms of mechanisms that produce item-method directed forgetting.

  16. A fast image retrieval method based on SVM and imbalanced samples in filtering multimedia message spam

    NASA Astrophysics Data System (ADS)

    Chen, Zhang; Peng, Zhenming; Peng, Lingbing; Liao, Dongyi; He, Xin

    2011-11-01

    With the swift and violent development of the Multimedia Messaging Service (MMS), it becomes an urgent task to filter the Multimedia Message (MM) spam effectively in real-time. For the fact that most MMs contain images or videos, a method based on retrieving images is given in this paper for filtering MM spam. The detection method used in this paper is a combination of skin-color detection, texture detection, and face detection, and the classifier for this imbalanced problem is a very fast multi-classification combining Support vector machine (SVM) with unilateral binary decision tree. The experiments on 3 test sets show that the proposed method is effective, with the interception rate up to 60% and the average detection time for each image less than 1 second.

  17. A Minimally Invasive Method for Retrieving Single Adherent Cells of Different Types from Cultures

    PubMed Central

    Zeng, Jia; Mohammadreza, Aida; Gao, Weimin; Merza, Saeed; Smith, Dean; Kelbauskas, Laimonas; Meldrum, Deirdre R.

    2014-01-01

    The field of single-cell analysis has gained a significant momentum over the last decade. Separation and isolation of individual cells is an indispensable step in almost all currently available single-cell analysis technologies. However, stress levels introduced by such manipulations remain largely unstudied. We present a method for minimally invasive retrieval of selected individual adherent cells of different types from cell cultures. The method is based on a combination of mechanical (shear flow) force and biochemical (trypsin digestion) treatment. We quantified alterations in the transcription levels of stress response genes in individual cells exposed to varying levels of shear flow and trypsinization. We report optimal temperature, RNA preservation reagents, shear force and trypsinization conditions necessary to minimize changes in the stress-related gene expression levels. The method and experimental findings are broadly applicable and can be used by a broad research community working in the field of single cell analysis. PMID:24957932

  18. Tropospheric CO Observations Using IASI datasets and an Optimal Estimation Retrieval Method

    NASA Astrophysics Data System (ADS)

    Illingworth, S. M.

    2009-04-01

    Carbon monoxide (CO) in the troposphere acts as a marker of large-scale influences of pollution on both the regional and global scale, whilst acting as a reference source for incomplete combustion. Through its reactions with the hydroxyl radical OH, the concentration of CO is also related to the oxidizing capacity of the troposphere, hence providing a chemical marker for the "local" chemical environment and its state. Investigations into perturbations of the sources, sinks and net surface fluxes of CO are therefore of increasing importance. CO exhibits strong locally elevated concentrations in the vicinity of sources due to its relatively short life time of weeks to months. Global measurements of CO from satellites will therefore allow to identify the main source regions and to quantify their source strength. The Infrared Atmospheric Sounding Interferometer (IASI) is a high-resolution (0.25 cm-1 unapodized) Michelson interferometer which was launched in 2007 on the European polar Meteorological Operational Platform (METEOP-1) satellite. The IASI instrument has a swath of about 2200 km, ensuring 99% global coverage twice a day, and is the first of a series of three instruments launched every five years, ensuring a continuity of data for a planned period of 15 years. The IASI instrument thus offers the possibility of high-spectral resolution infrared monitoring of CO over a very long time period. This work presents a new Optimal Estimation Method (OEM) retrieval of CO profiles in the mid-troposphere from observations of the IASI instrument. First retrieval results, as well as a validation of these results with ground-based sites and other satellites are included, and error characterization as well as the effects of noise in the retrievals is also discussed.

  19. Experimental Sentinel-2 LAI estimation using parametric, non-parametric and physical retrieval methods - A comparison

    NASA Astrophysics Data System (ADS)

    Verrelst, Jochem; Rivera, Juan Pablo; Veroustraete, Frank; Muñoz-Marí, Jordi; Clevers, Jan G. P. W.; Camps-Valls, Gustau; Moreno, José

    2015-10-01

    Given the forthcoming availability of Sentinel-2 (S2) images, this paper provides a systematic comparison of retrieval accuracy and processing speed of a multitude of parametric, non-parametric and physically-based retrieval methods using simulated S2 data. An experimental field dataset (SPARC), collected at the agricultural site of Barrax (Spain), was used to evaluate different retrieval methods on their ability to estimate leaf area index (LAI). With regard to parametric methods, all possible band combinations for several two-band and three-band index formulations and a linear regression fitting function have been evaluated. From a set of over ten thousand indices evaluated, the best performing one was an optimized three-band combination according to (ρ560 -ρ1610 -ρ2190) / (ρ560 +ρ1610 +ρ2190) with a 10-fold cross-validation RCV2 of 0.82 (RMSECV : 0.62). This family of methods excel for their fast processing speed, e.g., 0.05 s to calibrate and validate the regression function, and 3.8 s to map a simulated S2 image. With regard to non-parametric methods, 11 machine learning regression algorithms (MLRAs) have been evaluated. This methodological family has the advantage of making use of the full optical spectrum as well as flexible, nonlinear fitting. Particularly kernel-based MLRAs lead to excellent results, with variational heteroscedastic (VH) Gaussian Processes regression (GPR) as the best performing method, with a RCV2 of 0.90 (RMSECV : 0.44). Additionally, the model is trained and validated relatively fast (1.70 s) and the processed image (taking 73.88 s) includes associated uncertainty estimates. More challenging is the inversion of a PROSAIL based radiative transfer model (RTM). After the generation of a look-up table (LUT), a multitude of cost functions and regularization options were evaluated. The best performing cost function is Pearson's χ -square. It led to a R2 of 0.74 (RMSE: 0.80) against the validation dataset. While its validation went fast

  20. Analysis of sperm antigens by sodium dodecyl sulfate gel/protein blot radioimmunobinding method

    SciTech Connect

    Lee, C.Y.G.; Huang, Y.S.; Hu, P.C.; Gomel, V.; Menge, A.C.

    1982-06-01

    A radioimmunobinding method based on the blotting of renatured proteins from sodium dodecyl sulfate gels on to nitrocellulose filter papers was developed to analyze the sperm antigens that elicit serum anti-sperm antibodies. In rabbits, serum anti-sperm antibodies were raised by immunization with homologous epididymal spermatozoa mixed with complete Freund's adjuvant. The raised antisera from either male or female rabbits were shown to react with three major sperm protein bands on sodium dodecyl sulfate gels with the corresponding molecular weights of about 70,000 +/- 5000, 14,000, and 13,000, respectively. In humans, the monoclonal antibodies against human sperm were raised by a hybridoma technique. Out of six independent hybrid cell lines that were generated, three of them were shown to secrete immunoglobulins that react with the same two protein bands on sodium dodecyl sulfate gels, which have the approximate molecular weight of 10,000. The same procedure was also used to analyze human serum samples that were shown to contain anti-sperm antibodies by the known techniques. Unique sperm antigens that elicit anti-sperm antibodies in humans were identified and correlated. The results of this study suggest that sodium dodecyl sulfate gel/protein blot radioimmunobinding method may be a sensitive and useful tool for the study of sperm antigens that elicit autoimmune responses and their association with human infertility.

  1. Improved poliovirus D-antigen yields by application of different Vero cell cultivation methods.

    PubMed

    Thomassen, Yvonne E; Rubingh, Olaf; Wijffels, René H; van der Pol, Leo A; Bakker, Wilfried A M

    2014-05-19

    Vero cells were grown adherent to microcarriers (Cytodex 1; 3 g L(-1)) using animal component free media in stirred-tank type bioreactors. Different strategies for media refreshment, daily media replacement (semi-batch), continuous media replacement (perfusion) and recirculation of media, were compared with batch cultivation. Cell densities increased using a feed strategy from 1×10(6) cells mL(-1) during batch cultivation to 1.8, 2.7 and 5.0×10(6) cells mL(-1) during semi-batch, perfusion and recirculation, respectively. The effects of these different cell culture strategies on subsequent poliovirus production were investigated. Increased cell densities allowed up to 3 times higher D-antigen levels when compared with that obtained from batch-wise Vero cell culture. However, the cell specific D-antigen production was lower when cells were infected at higher cell densities. This cell density effect is in good agreement with observations for different cell lines and virus types. From the evaluated alternative culture methods, application of a semi-batch mode of operations allowed the highest cell specific D-antigen production. The increased product yields that can easily be reached using these higher cell density cultivation methods, showed the possibility for better use of bioreactor capacity for the manufacturing of polio vaccines to ultimately reduce vaccine cost per dose. Further, the use of animal-component-free cell- and virus culture media shows opportunities for modernization of human viral vaccine manufacturing.

  2. A Method to Estimate Crop Effects in Passive Microwave Soil Moisture Retrieval Above C-band

    NASA Astrophysics Data System (ADS)

    Zhang, Z.; Xu, Y.; Shi, J.

    2009-12-01

    moisture) directly to the observed Brightness Temperature. On the other hand, τ-ω model is simple in formula and are widely used in soil moisture retrieval at L- or C-band. To characterize ω and t of short vegetation above C-band, we setup a Brightness Temperature database with M-D model by a variety of parameters. Then results from M-D model were matched with those from τ-ω model at the same environmental conditions by least-square deviation, so as to retrieve ω and t of vegetation above C-band. To verify the derived effective single scattering albedo ω and transmissivity t, they were used, together with the Brightness Temperature data during SMEX02/PSR flight, in τ-ω model. The results of the equation were emissivity of soil, which were then used by a Qp model to retrieve soil moisture in the same area where PSR flight passed. The retrieved soil moisture matched the measurement very good, the rmse is 0.0458. This showed our method to retrieve ω and t are reliable.

  3. Tropospheric ozone retrieval by using SCIAMACHY Limb-Nadir-Matching method

    NASA Astrophysics Data System (ADS)

    Jia, Jia; Ladstätter-Weissenmayer, Annette; Ebojie, Felix; Rozanov, Alexei; Burrows, John

    2014-05-01

    Tropospheric ozone is photochemically produced during pollution events and transported from the stratosphere towards the troposphere. It is the third most important green house gases and the main component of summer smog. Global covered satellite measurements are well suitable to investigate sources, sinks, and transport mechanisms of tropospheric ozone in a global view, and to study a characteristic behaviour of the tropospheric ozone in regions. However, the usage of satellite data is associated to a large uncertainty as 90% ozone is located in the stratosphere and only the remaining part of 10% can be observed in the troposphere. The limb-nadir matching (LNM) technique is one of the methods suitable to retrieve tropospheric ozone distributions from space borne observations of the scattered solar light in the UV-visible spectral range. In this study we apply the LNM approach to alternating limb and nadir measurements performed by the SCIAMACHY instrument. A precise tropopause height is used to subtract the stratospheric ozone from the total ozone amount for each matching point. The focus of this work is to reduce the uncertainty of the resulting tropospheric ozone distributions by analysing possible error sources, refining both limb and nadir retrievals and the matching technique.

  4. Retrieval of water vapor mixing ratio from a multiple channel Raman-scatter lidar using an optimal estimation method.

    PubMed

    Sica, R J; Haefele, A

    2016-02-01

    Lidar measurements of the atmospheric water vapor mixing ratio provide an excellent complement to radiosoundings and passive, ground-based remote sensors. Lidars are now routinely used that can make high spatial-temporal resolution measurements of water vapor from the surface to the stratosphere. Many of these systems can operate during the day and night, with operation only limited by clouds thick enough to significantly attenuate the laser beam. To enhance the value of these measurements for weather and climate studies, this paper presents an optimal estimation method (OEM) to retrieve the water vapor mixing ratio, aerosol optical depth profile, Ångstrom exponent, lidar constants, detector dead times, and measurement backgrounds from multichannel vibrational Raman-scatter lidars. The OEM retrieval provides the systematic uncertainties due to the overlap function, calibration factor, air density and Rayleigh-scatter cross sections, in addition to the random uncertainties of the retrieval due to measurement noise. The OEM also gives the vertical resolution of the retrieval as a function of height, as well as the height to which the contribution of the a priori is small. The OEM is applied to measurements made by the Meteoswiss Raman Lidar for Meteorological Observations (RALMO) in the day and night for clear and cloudy conditions. The retrieved water vapor mixing ratio is in excellent agreement with both the traditional lidar retrieval method and coincident radiosoundings.

  5. A safe and efficient method to retrieve mesenchymal stem cells from three-dimensional fibrin gels.

    PubMed

    Carrion, Bita; Janson, Isaac A; Kong, Yen P; Putnam, Andrew J

    2014-03-01

    Mesenchymal stem cells (MSCs) display multipotent characteristics that make them ideal for potential therapeutic applications. MSCs are typically cultured as monolayers on tissue culture plastic, but there is increasing evidence suggesting that they may lose their multipotency over time in vitro and eventually cease to retain any resemblance to in vivo resident MSCs. Three-dimensional (3D) culture systems that more closely recapitulate the physiological environment of MSCs and other cell types are increasingly explored for their capacity to support and maintain the cell phenotypes. In much of our own work, we have utilized fibrin, a natural protein-based material that serves as the provisional extracellular matrix during wound healing. Fibrin has proven to be useful in numerous tissue engineering applications and has been used clinically as a hemostatic material. Its rapid self-assembly driven by thrombin-mediated alteration of fibrinogen makes fibrin an attractive 3D substrate, in which cells can adhere, spread, proliferate, and undergo complex morphogenetic programs. However, there is a significant need for simple cost-effective methods to safely retrieve cells encapsulated within fibrin hydrogels to perform additional analyses or use the cells for therapy. Here, we present a safe and efficient protocol for the isolation of MSCs from 3D fibrin gels. The key ingredient of our successful extraction method is nattokinase, a serine protease of the subtilisin family that has a strong fibrinolytic activity. Our data show that MSCs recovered from 3D fibrin gels using nattokinase are not only viable but also retain their proliferative and multilineage potentials. Demonstrated for MSCs, this method can be readily adapted to retrieve any other cell type from 3D fibrin gel constructs for various applications, including expansion, bioassays, and in vivo implantation.

  6. A Method to Retrieve Rainfall Rate Over Land from TRMM Microwave Imager Observations

    NASA Technical Reports Server (NTRS)

    Prabhakara, C.; Iacovazzi, R., Jr.; Yoo, J.-M.; Lau, William K. M. (Technical Monitor)

    2002-01-01

    Over tropical land regions, rain rate maxima in mesoscale convective systems revealed by the Precipitation Radar (PR) flown on the Tropical Rainfall Measuring Mission (TRMM) satellite are found to correspond to thunderstorms, i.e., Cbs. These Cbs are reflected as minima in the 85 GHz brightness temperature, T85, observed by the TRMM Microwave Imager (TMI) radiometer. Because the magnitude of TMI observations do not discriminate satisfactorily convective and stratiform rain, we developed here a different TMI discrimination method. In this method, two types of Cbs, strong and weak, are inferred from the Laplacian of T85 at minima. Then, to retrieve rain rate, where T85 is less than 270 K, a weak (background) rain rate is deduced using T85 observations. Furthermore, over a circular area of 10 km radius centered at the location of each T85 minimum, an additional Cb component of rain rate is added to the background rain rate. This Cb component of rain rate is estimated with the help of (T19-T37) and T85 observations. Initially, our algorithm is calibrated with the PR rain rate measurements from 20 MCS rain events. After calibration, this method is applied to TMI data taken from several tropical land regions. With the help of the PR observations, we show that the spatial distribution and intensity of rain rate over land estimated from our algorithm are better than those given by the current TMI-Version-5 Algorithm. For this reason, our algorithm may be used to improve the current state of rain retrievals on land.

  7. A Safe and Efficient Method to Retrieve Mesenchymal Stem Cells from Three-Dimensional Fibrin Gels

    PubMed Central

    Carrion, Bita; Janson, Isaac A.; Kong, Yen P.

    2014-01-01

    Mesenchymal stem cells (MSCs) display multipotent characteristics that make them ideal for potential therapeutic applications. MSCs are typically cultured as monolayers on tissue culture plastic, but there is increasing evidence suggesting that they may lose their multipotency over time in vitro and eventually cease to retain any resemblance to in vivo resident MSCs. Three-dimensional (3D) culture systems that more closely recapitulate the physiological environment of MSCs and other cell types are increasingly explored for their capacity to support and maintain the cell phenotypes. In much of our own work, we have utilized fibrin, a natural protein-based material that serves as the provisional extracellular matrix during wound healing. Fibrin has proven to be useful in numerous tissue engineering applications and has been used clinically as a hemostatic material. Its rapid self-assembly driven by thrombin-mediated alteration of fibrinogen makes fibrin an attractive 3D substrate, in which cells can adhere, spread, proliferate, and undergo complex morphogenetic programs. However, there is a significant need for simple cost-effective methods to safely retrieve cells encapsulated within fibrin hydrogels to perform additional analyses or use the cells for therapy. Here, we present a safe and efficient protocol for the isolation of MSCs from 3D fibrin gels. The key ingredient of our successful extraction method is nattokinase, a serine protease of the subtilisin family that has a strong fibrinolytic activity. Our data show that MSCs recovered from 3D fibrin gels using nattokinase are not only viable but also retain their proliferative and multilineage potentials. Demonstrated for MSCs, this method can be readily adapted to retrieve any other cell type from 3D fibrin gel constructs for various applications, including expansion, bioassays, and in vivo implantation. PMID:23808842

  8. Immunoelectron microscopy of skin basement membrane zone antigens: a pre-embedding method using 1-nm immunogold with silver enhancement.

    PubMed

    McGrath, J A; Ishida-Yamamoto, A; Shimizu, H; Fine, J D; Eady, R A

    1994-05-01

    There is no single immunoelectron microscopical method for invariably effective localization of both intracellular and extracellular antigens. We describe a simple and practicable immunogold technique that can be used to localize various skin basement membrane zone antigens at the ultrastructural level. Small pieces of skin were incubated with primary antibodies recognizing epitopes on a range of basement membrane zone-related antigens (two different lamina lucida-associated antigens, laminin, type VII collagen, fibrillin and keratin 14). This was followed by incubation with 1-nm colloidal gold-conjugated secondary antibody and subsequent silver intensification. The specimens were then processed for transmission electron microscopy. Precise immunolocalization with good ultrastructural preservation was achieved for all basement membrane zone antibodies tested. The results of basal cell keratin immunostaining showed that this microscopic approach could also be applied to some extent in the characterization of intracellular antigens. This immunoelectron microscopy technique provides a useful approach to the study of macromolecules at the basement membrane zone.

  9. Demonstration of retrieval methods for Westinghouse Hanford Corporation October 20, 1995

    SciTech Connect

    1996-10-01

    Westinghouse Hanford Corporation has been pursuing strategies to break up and retrieve the radioactive waste material in single shell storage tanks at the Hanford Nuclear Reservation, by working with non-radioactive ``saltcake`` and sludge material that simulate the actual waste. It has been suggested that the use of higher volumes of water than used in the past (10 gpm nozzles at 10,000 psi) might be successful in breaking down the hard waste simulants. Additionally, the application of these higher volumes of water might successfully be applied through commercially available tooling using methods similar to those used in the deslagging of large utility boilers. NMW Industrial Services, Inc., has proposed a trial consisting of three approaches each to dislodging both the solid (saltcake) simulant and the sludge simulant.

  10. Retrieval practice is an efficient method of enhancing the retention of anatomy and physiology information.

    PubMed

    Dobson, John L

    2013-06-01

    Although a great deal of empirical evidence has indicated that retrieval practice is an effective means of promoting learning and memory, very few studies have investigated the strategy in the context of an actual class. The primary purpose of this study was to determine if a series of very brief retrieval quizzes could significantly improve the retention of previously tested information throughout an anatomy and physiology course. A second purpose was to determine if there were any significant differences between expanding and uniform patterns of retrieval that followed a standardized initial retrieval delay. Anatomy and physiology students were assigned to either a control group or groups that were repeatedly prompted to retrieve a subset of previously tested course information via a series of quizzes that were administered on either an expanding or a uniform schedule. Each retrieval group completed a total of 10 retrieval quizzes, and the series of quizzes required (only) a total of 2 h to complete. Final retention of the exam subset material was assessed during the last week of the semester. There were no significant differences between the expanding and uniform retrieval groups, but both retained an average of 41% more of the subset material than did the control group (ANOVA, F = 129.8, P = 0.00, ηp(2) = 0.36). In conclusion, retrieval practice is a highly efficient and effective strategy for enhancing the retention of anatomy and physiology material.

  11. Method for indexing and retrieving manufacturing-specific digital imagery based on image content

    DOEpatents

    Ferrell, Regina K.; Karnowski, Thomas P.; Tobin, Jr., Kenneth W.

    2004-06-15

    A method for indexing and retrieving manufacturing-specific digital images based on image content comprises three steps. First, at least one feature vector can be extracted from a manufacturing-specific digital image stored in an image database. In particular, each extracted feature vector corresponds to a particular characteristic of the manufacturing-specific digital image, for instance, a digital image modality and overall characteristic, a substrate/background characteristic, and an anomaly/defect characteristic. Notably, the extracting step includes generating a defect mask using a detection process. Second, using an unsupervised clustering method, each extracted feature vector can be indexed in a hierarchical search tree. Third, a manufacturing-specific digital image associated with a feature vector stored in the hierarchicial search tree can be retrieved, wherein the manufacturing-specific digital image has image content comparably related to the image content of the query image. More particularly, can include two data reductions, the first performed based upon a query vector extracted from a query image. Subsequently, a user can select relevant images resulting from the first data reduction. From the selection, a prototype vector can be calculated, from which a second-level data reduction can be performed. The second-level data reduction can result in a subset of feature vectors comparable to the prototype vector, and further comparable to the query vector. An additional fourth step can include managing the hierarchical search tree by substituting a vector average for several redundant feature vectors encapsulated by nodes in the hierarchical search tree.

  12. An automatic Planetary Boundary Layer height retrieval method with compact EZ backscattering Lidar

    NASA Astrophysics Data System (ADS)

    Loaec, S.; Sauvage, L.; Boquet, M.; Lolli, S.; Rouget, V.

    2009-09-01

    Bigger strongly urbanized cities in the world are often exposed to atmospheric pollution events. To understand the chemical and physical processes that are taking place in these areas it is necessary to describe correctly the Planetary Boundary Layer (PBL) dynamics and the PBL height evolution. For these proposals, a compact and rugged eye safe UV Lidar, the EZLIDAR™, was developed together by CEA/LMD and LEOSPHERE (France) to study and investigate structural and optical properties of clouds and aerosols and PBL time evolution. EZLIDAR™ has been validated by different remote and in-situ instruments as MPL Type-4 Lidar manufactured by NASA at ARM/SGP site or the LNA (Lidar Nuage Aerosol) at the Laboratoire de Metereologie Dynamique LMD (France) and during several intercomparison campaigns. EZLIDAR™ algorithm retrieves automatically the PBL height in real-time. The method is based on the detection of the slope of the signal linked to a sharp change in concentration of the aerosols. Once detected, the different layers are filtered on a 15mn sample and classified between nocturnal, convective or residual layer, depending on the time and date. This method has been validated against those retrieved by the algorithm STRAT from data acquired at IPSL, France, showing 95% of correlation. In this paper are presented the results of the intercomparison campaign that took place in Orleans, France and Mace Head, Ireland in the framework of ICOS (Integrated Carbon Observation System) project, where the EZ Lidar™ worked under all weather conditions, clear sky, fog, low clouds, during the whole month of October 2008. Moreover, thanks to its 3D scanning capability, the EZLIDAR was able to provide the variability of the PBL height around the site, enabling the scientists to estimate the flux intensities that play a key role in the radiative transfer budget and in the atmospheric pollutants dispersion.

  13. Stabilization of Transfected Cells Expressing Low-Incidence Blood Group Antigens: Novel Methods Facilitating Their Use as Reagent-Cells

    PubMed Central

    González, Cecilia; Esteban, Rosa; Canals, Carme; Muñiz-Díaz, Eduardo; Nogués, Núria

    2016-01-01

    Background The identification of erythrocyte antibodies in the serum of patients rely on panels of human red blood cells (RBCs), which coexpress many antigens and are not easily available for low-incidence blood group phenotypes. These problems have been addressed by generating cell lines expressing unique blood group antigens, which may be used as an alternative to human RBCs. However, the use of cell lines implies several drawbacks, like the requirement of cell culture facilities and the high cost of cryopreservation. The application of cell stabilization methods could facilitate their use as reagent cells in clinical laboratories. Methods We generated stably-transfected cells expressing low-incidence blood group antigens (Dia and Lua). High-expresser clones were used to assess the effect of TransFix® treatment and lyophilization as cell preservation methods. Cells were kept at 4°C and cell morphology, membrane permeability and antigenic properties were evaluated at several time-points after treatment. Results TransFix® addition to cell suspensions allows cell stabilization and proper antigen detection for at least 120 days, despite an increase in membrane permeability and a reduction in antigen expression levels. Lyophilized cells showed minor morphological changes and antigen expression levels were rather conserved at days 1, 15 and 120, indicating a high stability of the freeze-dried product. These stabilized cells have been proved to react specifically with human sera containing alloantibodies. Conclusions Both stabilization methods allow long-term preservation of the transfected cells antigenic properties and may facilitate their distribution and use as reagent-cells expressing low-incidence antigens, overcoming the limited availability of such rare RBCs. PMID:27603310

  14. Content based Image Retrieval based on Different Global and Local Color Histogram Methods: A Survey

    NASA Astrophysics Data System (ADS)

    Suhasini, Pallikonda Sarah; Sri Rama Krishna, K.; Murali Krishna, I. V.

    2016-06-01

    Different global and local color histogram methods for content based image retrieval (CBIR) are investigated in this paper. Color histogram is a widely used descriptor for CBIR. Conventional method of extracting color histogram is global, which misses the spatial content, is less invariant to deformation and viewpoint changes, and results in a very large three dimensional histogram corresponding to the color space used. To address the above deficiencies, different global and local histogram methods are proposed in recent research. Different ways of extracting local histograms to have spatial correspondence, invariant colour histogram to add deformation and viewpoint invariance and fuzzy linking method to reduce the size of the histogram are found in recent papers. The color space and the distance metric used are vital in obtaining color histogram. In this paper the performance of CBIR based on different global and local color histograms in three different color spaces, namely, RGB, HSV, L*a*b* and also with three distance measures Euclidean, Quadratic and Histogram intersection are surveyed, to choose appropriate method for future research.

  15. Validation of GOES-Derived Surface Radiation Using NOAA's Physical Retrieval Method

    SciTech Connect

    Habte, A.; Sengupta, M.; Wilcox, S.

    2013-01-01

    This report was part of a multiyear collaboration with the University of Wisconsin and the National Oceanic and Atmospheric Administration (NOAA) to produce high-quality, satellite-based, solar resource datasets for the United States. High-quality, solar resource assessment accelerates technology deployment by making a positive impact on decision making and reducing uncertainty in investment decisions. Satellite-based solar resource datasets are used as a primary source in solar resource assessment. This is mainly because satellites provide larger areal coverage and longer periods of record than ground-based measurements. With the advent of newer satellites with increased information content and faster computers that can process increasingly higher data volumes, methods that were considered too computationally intensive are now feasible. One class of sophisticated methods for retrieving solar resource information from satellites is a two-step, physics-based method that computes cloud properties and uses the information in a radiative transfer model to compute solar radiation. This method has the advantage of adding additional information as satellites with newer channels come on board. This report evaluates the two-step method developed at NOAA and adapted for solar resource assessment for renewable energy with the goal of identifying areas that can be improved in the future.

  16. Content based Image Retrieval based on Different Global and Local Color Histogram Methods: A Survey

    NASA Astrophysics Data System (ADS)

    Suhasini, Pallikonda Sarah; Sri Rama Krishna, K.; Murali Krishna, I. V.

    2017-02-01

    Different global and local color histogram methods for content based image retrieval (CBIR) are investigated in this paper. Color histogram is a widely used descriptor for CBIR. Conventional method of extracting color histogram is global, which misses the spatial content, is less invariant to deformation and viewpoint changes, and results in a very large three dimensional histogram corresponding to the color space used. To address the above deficiencies, different global and local histogram methods are proposed in recent research. Different ways of extracting local histograms to have spatial correspondence, invariant colour histogram to add deformation and viewpoint invariance and fuzzy linking method to reduce the size of the histogram are found in recent papers. The color space and the distance metric used are vital in obtaining color histogram. In this paper the performance of CBIR based on different global and local color histograms in three different color spaces, namely, RGB, HSV, L*a*b* and also with three distance measures Euclidean, Quadratic and Histogram intersection are surveyed, to choose appropriate method for future research.

  17. Retrieval of the pulse amplitude and phase from cross-phase modulation spectrograms using the simulated annealing method.

    PubMed

    Honzatko, Pavel; Kanka, J; Vrany, B

    2004-11-29

    The simulated annealing method is used for retrieving the amplitude and phase from cross-phase modulation spectrograms. The method allows us to take into account the birefringence of the measurement fiber and resolution of the optical spectrum analyzer. The influence of the birefringence and analyzer resolution are discussed.

  18. A linear method for the retrieval of sun-induced chlorophyll fluorescence from GOME-2 and SCIAMACHY data

    NASA Astrophysics Data System (ADS)

    Köhler, Philipp; Guanter, Luis; Joiner, Joanna

    2015-04-01

    Global retrievals of near-infrared sun-induced chlorophyll fluorescence (SIF) have been achieved in the last several years by means of space-borne atmospheric spectrometers. SIF is an electromagnetic signal emitted by the chlorophyll-a of photosynthetically active vegetation in the 650-850 nm spectral range. It represents a part of the excess energy during the process of photosynthesis and provides a measure of photosynthetic activity. The key challenge to retrieve SIF from space is to isolate the signal from the about 100 times more intense reflected solar radiation in the measured top of atmosphere (TOA) radiance spectrum. Nevertheless, it has been demonstrated that a number of satellite sensors provide the necessary spectral and radiometric performance to evaluate the in-filling of solar Fraunhofer lines and/or atmospheric absorption features by SIF. We will present recent developments for the retrieval of SIF from medium spectral resolution space-borne spectrometers such as the Global Ozone Monitoring Experiment (GOME-2) and the Scanning Imaging Absorption SpectroMeter for Atmospheric ChartographY (SCIAMACHY). Building upon the previous work by Joiner et al. 2013, our approach solves existing issues in the retrieval such as the non-linearity of the forward model and the arbitrary selection of the number of free parameters. In particular, we use a backward elimination algorithm to optimize the number of coefficients to fit, which reduces also the retrieval noise and selects the number of state vector elements automatically. A sensitivity analysis with simulated spectra has been utilized to evaluate the performance of our retrieval approach. The method has also been applied to estimate SIF from real spectra from GOME-2 and for the first time, from SCIAMACHY. We are able to present a time series of GOME-2 SIF results covering the 2007-2011 time period and SCIAMACHY SIF results between 2003-2011. This represents an almost one decade long record of global SIF. We

  19. Latent Semantic Analysis as a Method of Content-Based Image Retrieval in Medical Applications

    ERIC Educational Resources Information Center

    Makovoz, Gennadiy

    2010-01-01

    The research investigated whether a Latent Semantic Analysis (LSA)-based approach to image retrieval can map pixel intensity into a smaller concept space with good accuracy and reasonable computational cost. From a large set of M computed tomography (CT) images, a retrieval query found all images for a particular patient based on semantic…

  20. An automatic method for retrieving and indexing catalogues of biomedical courses.

    PubMed

    Maojo, Victor; de la Calle, Guillermo; García-Remesal, Miguel; Bankauskaite, Vaida; Crespo, Jose

    2008-11-06

    Although there is wide information about Biomedical Informatics education and courses in different Websites, information is usually not exhaustive and difficult to update. We propose a new methodology based on information retrieval techniques for extracting, indexing and retrieving automatically information about educational offers. A web application has been developed to make available such information in an inventory of courses and educational offers.

  1. Retrieval Practice Is an Efficient Method of Enhancing the Retention of Anatomy and Physiology Information

    ERIC Educational Resources Information Center

    Dobson, John L.

    2013-01-01

    Although a great deal of empirical evidence has indicated that retrieval practice is an effective means of promoting learning and memory, very few studies have investigated the strategy in the context of an actual class. The primary purpose of this study was to determine if a series of very brief retrieval quizzes could significantly improve the…

  2. The impact of the choice of radiative transfer model and inversion method on the OSIRIS ozone and nitrogen dioxide retrievals

    NASA Astrophysics Data System (ADS)

    Haley, Craig; McLinden, Chris; Sioris, Christopher; Brohede, Samuel

    Key to the retrieval of stratospheric minor species information from limb-scatter measurements are the selections of a radiative transfer model (RTM) and inversion method (solver). Here we assess the impact of choice of RTM and solver on the retrievals of stratospheric ozone and nitrogen dioxide from the OSIRIS instrument using the ‘Ozone Triplet' and Differential Optical Absorption Spectroscopy (DOAS) techniques that are used in the operational Level 2 processing algorithms. The RTMs assessed are LIMBTRAN, VECTOR, SCIARAYS, and SASKTRAN. The solvers studied include the Maximum A Posteriori (MAP), Maximum Likelihood (ML), Iterative Least Squares (ILS), and Chahine methods.

  3. Real-time measurement of laser beam quality factor by the Fresnel phase-retrieval method

    NASA Astrophysics Data System (ADS)

    Yang, Pao-Keng; Liu, Jian-You; Chen, Yung-Chieh; Hsu, Chia-En

    2016-09-01

    Conventionally, it is a tedious work to measure the beam quality factor for a laser beam because one needs to move a camera-based beam profiler from one location to another for many times to record intensity profiles at different positions around the beam waist. We present a simple method for determining the laser beam quality factor from only two laser intensity profiles at different cross sections around the waist. We first used an iterative phase-retrieval algorithm, based on the Huygens-Fresnel principle, to reconstruct the phase profiles at the two cross sections where the intensity profiles had been measured. Once the optical field amplitude (the square root of intensity) and phase distribution functions at certain cross section of a laser beam had been determined, we can propagate the light wave at this cross section by using the Fresnel diffraction formula to obtain the intensity profiles at different positions, from which the beam quality factor can be determined. Using a HeNe laser for test, we had experimentally demonstrated the feasibility of our idea by showing that the result from our proposed method is in good agreement with that obtained from the conventional method. Our setup is capable of executing a real-time measurement of the beam quality factor because the two intensity profiles can be simultaneously recorded by using a beam splitter and two beam-profilers controlled by the same computer.

  4. Active on-demand service method based on event-driven architecture for geospatial data retrieval

    NASA Astrophysics Data System (ADS)

    Fan, Minghu; Fan, Hong; Chen, Nengcheng; Chen, Zeqiang; Du, Wu

    2013-07-01

    Timely on-demand access to geospatial data is necessary for environmental observation and disaster response. However, traditional service methods for acquiring geospatial data are inefficient and cumbersome, which is not beneficial for timely data acquisition. In these service methods, data are obtained and published by managers and are then left to users to discover and to retrieve them. To solve this problem, we propose an event-driven active on-demand data service method, for which a prototype based on sensor web technologies is demonstrated. First, we select a subset of observed properties as the attributes of an observation event of a data service system. Event-filtering technologies are then employed to find the data desired by users. Finally, the data that meet the subscription requirement are pushed to subscribers on time. The aims of the implementation of the method are to test the suitability of the observation and measurement (O&M) profile for Earth observation and OGC event pattern markup language (EML) specification. We determined the attributes of observation events according to the requirement of the data service and encoded observation event information using the OGC Observations and Measurements specification. We encoded the information under filtering conditions using the OGC Event Pattern Markup Language specification. We implemented a data service method that is based on event-driven architecture via a combination of some sensor web enablement services. Finally, we verified the feasibility of the method using MODIS data from the forest fires that occurred on February 7, 2009, in Victoria, Australia. The results show that the proposed method can achieve actively pushing the desired data to subscribers in the shortest possible time. O&M profiles for Earth observation and EML are suitable for the metadata encoding of observation events and the encoding of subscription information respectively. They match well for the data service in the system.

  5. Characterization of freshly retrieved preantral follicles using a low-invasive, mechanical isolation method extended to different ruminant species.

    PubMed

    Langbeen, A; Jorssen, E P A; Fransen, E; Rodriguez, A P A; García, M Chong; Leroy, J L M R; Bols, P E J

    2015-10-01

    Due to the increased interest in preantral follicular physiology, non-invasive retrieval and morphological classification are crucial. Therefore, this study aimed: (1) to standardize a minimally invasive isolation protocol, applicable to three ruminant species; (2) to morphologically classify preantral follicles upon retrieval; and (3) to describe morphological features of freshly retrieved follicles compared with follicle characteristics using invasive methods. Bovine, caprine and ovine ovarian cortex strips were retrieved from slaughterhouse ovaries and dispersed. This suspension was filtered, centrifuged, re-suspended and transferred to a Petri dish, to which 0.025 mg/ml neutral red (NR) was added to assess the viability of the isolated follicles. Between 59 and 191 follicles per follicle class and per species were collected and classified by light microscopy, based on follicular cell morphology. Subsequently, follicle diameters were measured. The proposed isolation protocol was applicable to all three species and showed a significant, expected increase in diameter with developmental stage. With an average diameter of 37 ± 5 μm for primordial follicles, 47 ± 6.3 μm for primary follicles and 67.1 ± 13.1 μm for secondary follicles, no significant difference in diameter among the three species was observed. Bovine, caprine and ovine follicles (63, 59 and 50% respectively) were graded as viable upon retrieval. Using the same morphological characteristics as determined by invasive techniques [e.g. haematoxylin-eosin (HE) sections], cumulus cell morphology and follicle diameter could be used routinely to classify freshly retrieved follicles. Finally, we applied a mechanical, minimally invasive, follicle isolation protocol and extended it to three ruminant species, yielding viable preantral follicles without compromising further in vitro processing and allowing routine follicle characterization upon retrieval.

  6. Use of thermal infrared pictures for retrieving intertidal DEM by the waterline method: advantages and limitations

    NASA Astrophysics Data System (ADS)

    Gaudin, D.; Delacourt, C.; Allemand, P.

    2010-12-01

    Digital Elevation Models (DEM) of the intertidal zones have a growing interest for ecological and land development purposes. They are also a fundamental tool for monitoring current sedimentary movements in those low energy environments. Such DEMs have to be constructed with a centimetric resolution as the topographic changes are not predictable and as sediment displacements are weak. Direct construction of DEM by GPS in these muddy environment is difficult: photogrammetric techniques are not efficient on uniform coloured surfaces and terrestrial laser scans are difficult to stabilize on the mud, due to humidity. In this study, we propose to improve and to apply the waterline method to retrieve DEMs in intertidal zones. This technique is based on monitoring accurately the boundary between sand and water during a whole tide rise with thermal infrared images. The DEM is made by stacking all these lines calibrated by an immersed pressure sensor. Using thermal infrared pictures, instead of optical ones, improves the detection of the waterline, since mud and water have very different responses to sun heating and a large emissivity contrast. However, temperature retrieving from thermal infrared data is not trivial, since the luminance of an object is the sum of a radiative part and a reflexive part, whose relative proportions are given by the emissivity. In the following equation, B accounts for the equivalent blackbody luminance, and Linc is the incident luminance : Ltot}=L{rad}+L_{refl=ɛ B+(1-ɛ )Linc The infrared waterline technique has been used for the monitoring of a beach located on the Aber Benoit, 8.5km away from the open sea. The site is mainly constituted of mud, and waves are very small (less than one centimeter high), which are the ideal conditions for using the waterline method. A few measurements have been made to make differential heigh maps of sediments. We reached a mean resolution of 2cm and a vertical accuracy better than one centimeter. The results

  7. Automatic query generation using word embeddings for retrieving passages describing experimental methods

    PubMed Central

    Aydın, Ferhat; Hüsünbeyi, Zehra Melce; Özgür, Arzucan

    2017-01-01

    Information regarding the physical interactions among proteins is crucial, since protein–protein interactions (PPIs) are central for many biological processes. The experimental techniques used to verify PPIs are vital for characterizing and assessing the reliability of the identified PPIs. A lot of information about PPIs and the experimental methods are only available in the text of the scientific publications that report them. In this study, we approach the problem of identifying passages with experimental methods for physical interactions between proteins as an information retrieval search task. The baseline system is based on query matching, where the queries are generated by utilizing the names (including synonyms) of the experimental methods in the Proteomics Standard Initiative–Molecular Interactions (PSI-MI) ontology. We propose two methods, where the baseline queries are expanded by including additional relevant terms. The first method is a supervised approach, where the most salient terms for each experimental method are obtained by using the term frequency–relevance frequency (tf.rf) metric over 13 articles from our manually annotated data set of 30 full text articles, which is made publicly available. On the other hand, the second method is an unsupervised approach, where the queries for each experimental method are expanded by using the word embeddings of the names of the experimental methods in the PSI-MI ontology. The word embeddings are obtained by utilizing a large unlabeled full text corpus. The proposed methods are evaluated on the test set consisting of 17 articles. Both methods obtain higher recall scores compared with the baseline, with a loss in precision. Besides higher recall, the word embeddings based approach achieves higher F-measure than the baseline and the tf.rf based methods. We also show that incorporating gene name and interaction keyword identification leads to improved precision and F-measure scores for all three evaluated

  8. Regional content-based image retrieval for solar images: Traditional versus modern methods

    NASA Astrophysics Data System (ADS)

    Banda, J. M.; Angryk, R. A.

    2015-11-01

    This work presents an extensive evaluation between conventional (distance-based) and modern (search-engine) information retrieval techniques in the context of finding similar Solar image regions within the Solar Dynamics Observatory (SDO) mission image repository. We compare pre-computed image descriptors (image features) extracted from the SDO mission images in two very different ways: (1) similarity retrieval using multiple distance-based metrics and (2) retrieval using Lucene, a general purpose scalable retrieval engine. By transforming image descriptors into histogram-like signatures and into Lucene-compatible text strings, we are able to effectively evaluate the retrieval capabilities of both methodologies. Using the image descriptors alongside a labeled image dataset, we present an extensive evaluation under the criteria of performance, scalability and retrieval precision of experimental retrieval systems in order to determine which implementation would be ideal for a production level system. In our analysis we performed key transformations to our sample datasets to properly evaluate rotation invariance and scalability. At the end of this work we conclude which technique is the most robust and would yield the best performing system after an extensive experimental evaluation, we also point out the strengths and weaknesses of each approach and theorize on potential improvements.

  9. A novel method for efficient archiving and retrieval of biomedical images using MPEG-7

    NASA Astrophysics Data System (ADS)

    Meyer, Joerg; Pahwa, Ash

    2004-10-01

    Digital archiving and efficient retrieval of radiological scans have become critical steps in contemporary medical diagnostics. Since more and more images and image sequences (single scans or video) from various modalities (CT/MRI/PET/digital X-ray) are now available in digital formats (e.g., DICOM-3), hospitals and radiology clinics need to implement efficient protocols capable of managing the enormous amounts of data generated daily in a typical clinical routine. We present a method that appears to be a viable way to eliminate the tedious step of manually annotating image and video material for database indexing. MPEG-7 is a new framework that standardizes the way images are characterized in terms of color, shape, and other abstract, content-related criteria. A set of standardized descriptors that are automatically generated from an image is used to compare an image to other images in a database, and to compute the distance between two images for a given application domain. Text-based database queries can be replaced with image-based queries using MPEG-7. Consequently, image queries can be conducted without any prior knowledge of the keys that were used as indices in the database. Since the decoding and matching steps are not part of the MPEG-7 standard, this method also enables searches that were not planned by the time the keys were generated.

  10. One Question, Multiple Answers: Biochemical and Biophysical Screening Methods Retrieve Deviating Fragment Hit Lists.

    PubMed

    Schiebel, Johannes; Radeva, Nedyalka; Köster, Helene; Metz, Alexander; Krotzky, Timo; Kuhnert, Maren; Diederich, Wibke E; Heine, Andreas; Neumann, Lars; Atmanene, Cedric; Roecklin, Dominique; Vivat-Hannah, Valérie; Renaud, Jean-Paul; Meinecke, Robert; Schlinck, Nina; Sitte, Astrid; Popp, Franziska; Zeeb, Markus; Klebe, Gerhard

    2015-09-01

    Fragment-based lead discovery is gaining momentum in drug development. Typically, a hierarchical cascade of several screening techniques is consulted to identify fragment hits which are then analyzed by crystallography. Because crystal structures with bound fragments are essential for the subsequent hit-to-lead-to-drug optimization, the screening process should distinguish reliably between binders and non-binders. We therefore investigated whether different screening methods would reveal similar collections of putative binders. First we used a biochemical assay to identify fragments that bind to endothiapepsin, a surrogate for disease-relevant aspartic proteases. In a comprehensive screening approach, we then evaluated our 361-entry library by using a reporter-displacement assay, saturation-transfer difference NMR, native mass spectrometry, thermophoresis, and a thermal shift assay. While the combined results of these screening methods retrieve 10 of the 11 crystal structures originally predicted by the biochemical assay, the mutual overlap of individual hit lists is surprisingly low, highlighting that each technique operates on different biophysical principles and conditions.

  11. Recursive method for phase retrieval using transport of intensity and its applications.

    PubMed

    Basunia, Mahmudunnabi; Banerjee, Partha P; Abeywickrema, Ujitha; Poon, Ting-Chung; Zhang, Hongbo

    2016-11-20

    Propagation of optical fields is governed by the Helmholtz equation or the paraxial wave equation. Transport of intensity is a noninterferometric method to find the phase of an object by recording optical intensities at different distances of propagation. The transport of intensity equation results from the imaginary part of the complex paraxial wave equation and is equivalent to the principle of conservation of energy. The real part of the paraxial wave equation yields the Eikonal equation in the presence of diffraction. The amplitude and phase of the optical field must therefore simultaneously satisfy both the real and imaginary parts of the paraxial wave equation during propagation. In this paper, we demonstrate, with illustrative examples, how to exploit this to retrieve the phase through recursive calculations of the phase and intensity. This is achieved using the transport of intensity equation, which is solved using standard techniques, and the real part of the paraxial wave equation, or the transport of phase equation, which is solved using a Gauss-Seidel iterative method. Examples include calculation of the imaged phase induced through self-phase modulation of a focused laser beam in a liquid and the imaged phase of light reflected from a surface, which yields the 3D surface profile.

  12. Retrieving Leaf Area Index (LAI) Using Remote Sensing: Theories, Methods and Sensors

    PubMed Central

    Zheng, Guang; Moskal, L. Monika

    2009-01-01

    The ability to accurately and rapidly acquire leaf area index (LAI) is an indispensable component of process-based ecological research facilitating the understanding of gas-vegetation exchange phenomenon at an array of spatial scales from the leaf to the landscape. However, LAI is difficult to directly acquire for large spatial extents due to its time consuming and work intensive nature. Such efforts have been significantly improved by the emergence of optical and active remote sensing techniques. This paper reviews the definitions and theories of LAI measurement with respect to direct and indirect methods. Then, the methodologies for LAI retrieval with regard to the characteristics of a range of remotely sensed datasets are discussed. Remote sensing indirect methods are subdivided into two categories of passive and active remote sensing, which are further categorized as terrestrial, aerial and satellite-born platforms. Due to a wide variety in spatial resolution of remotely sensed data and the requirements of ecological modeling, the scaling issue of LAI is discussed and special consideration is given to extrapolation of measurement to landscape and regional levels. PMID:22574042

  13. Retrieving Leaf Area Index (LAI) Using Remote Sensing: Theories, Methods and Sensors.

    PubMed

    Zheng, Guang; Moskal, L Monika

    2009-01-01

    The ability to accurately and rapidly acquire leaf area index (LAI) is an indispensable component of process-based ecological research facilitating the understanding of gas-vegetation exchange phenomenon at an array of spatial scales from the leaf to the landscape. However, LAI is difficult to directly acquire for large spatial extents due to its time consuming and work intensive nature. Such efforts have been significantly improved by the emergence of optical and active remote sensing techniques. This paper reviews the definitions and theories of LAI measurement with respect to direct and indirect methods. Then, the methodologies for LAI retrieval with regard to the characteristics of a range of remotely sensed datasets are discussed. Remote sensing indirect methods are subdivided into two categories of passive and active remote sensing, which are further categorized as terrestrial, aerial and satellite-born platforms. Due to a wide variety in spatial resolution of remotely sensed data and the requirements of ecological modeling, the scaling issue of LAI is discussed and special consideration is given to extrapolation of measurement to landscape and regional levels.

  14. Comparative analysis of different retrieval methods for mapping grassland leaf area index using airborne imaging spectroscopy

    NASA Astrophysics Data System (ADS)

    Atzberger, Clement; Darvishzadeh, Roshanak; Immitzer, Markus; Schlerf, Martin; Skidmore, Andrew; le Maire, Guerric

    2015-12-01

    Fine scale maps of vegetation biophysical variables are useful status indicators for monitoring and managing national parks and endangered habitats. Here, we assess in a comparative way four different retrieval methods for estimating leaf area index (LAI) in grassland: two radiative transfer model (RTM) inversion methods (one based on look-up-tables (LUT) and one based on predictive equations) and two statistical modelling methods (one partly, the other entirely based on in situ data). For prediction, spectral data were used that had been acquired over Majella National Park in Italy by the airborne hyperspectral HyMap instrument. To assess the performance of the four investigated models, the normalized root mean squared error (nRMSE) and coefficient of determination (R2) between estimates and in situ LAI measurements are reported (n = 41). Using a jackknife approach, we also quantified the accuracy and robustness of empirical models as a function of the size of the available calibration data set. The results of the study demonstrate that the LUT-based RTM inversion yields higher accuracies for LAI estimation (R2 = 0.91, nRMSE = 0.18) as compared to RTM inversions based on predictive equations (R2 = 0.79, nRMSE = 0.38). The two statistical methods yield accuracies similar to the LUT method. However, as expected, the accuracy and robustness of the statistical models decrease when the size of the calibration database is reduced to fewer samples. The results of this study are of interest for the remote sensing community developing improved inversion schemes for spaceborne hyperspectral sensors applicable to different vegetation types. The examples provided in this paper may also serve as illustrations for the drawbacks and advantages of physical and empirical models.

  15. Method of retrieving a liquid sample, a suction lysimeter, a portable suction lysimeter, a lysimeter system, and a deep lysimeter

    DOEpatents

    Hubbell, Joel M.; Sisson, James B.

    2003-08-26

    A method of retrieving a liquid sample comprises providing a portable lysimeter including a semi-permeable membrane and a chamber in fluid communication with the semi-permeable membrane; making a hole at a site from which a liquid sample is desired; evacuating the chamber by applying a vacuum to the chamber; lowering the portable lysimeter into the hole; obtaining a sample in the chamber; and retrieving the lysimeter from the bore; wherein it is not necessary to backfill the bore. A portable lysimeter includes a semi-permeable member and a chamber in fluid communication with the semi-permeable membrane.

  16. A novel method for rapid detection of Streptococcus pneumoniae antigens in blood.

    PubMed

    Fukushima, Kiyoyasu; Kubo, Toru; Ehara, Naomi; Nakano, Reiji; Matsutake, Toyoshi; Ishimatu, Yuji; Tanaka, Yumi; Akamatsu, Suguru; Izumikawa, Koichi; Kohno, Shigeru

    2016-03-01

    In this study, we used "RAPIRUN(®)Streptococcus pneumoniae HS (otitis media/sinusitis) (RAPIRUN-HS)," a rapid S. pneumoniae antigen detection kit, to investigate methods for detecting S. pneumoniae antigens in blood of 32 bacterial pneumonia patients. We simultaneously performed PCR to detect S. pneumoniae in blood samples. The results of these tests were compared based on pneumonia severity, determined using the Pneumonia Severity Index (PSI) score classification. Four S. pneumoniae PCR-positive patients of the six severe pneumococcal pneumonia patients (PSI risk class IV/V) also tested positive using RAPIRUN-HS. Twenty-four mild to moderate pneumonia patients (PSI risk class I-III) were S. pneumoniae PCR-negative; of these, 21 tested negative using RAPIRUN-HS. The pneumococcal pneumonia patients testing positive using RAPIRUN-HS had low leukocyte counts and elevated C-reactive protein and procalcitonin levels, indicating that RAPIRUN-HS results were correlated with pneumonia severity. The time course evaluations of the laboratory tests for severe pneumococcal pneumonia patients showed that RAPIRUN-HS and S. pneumoniae PCR yielded positive results earlier than the changes in procalcitonin and IL-6. Thus, concomitant pneumococcal bacteremia was strongly suspected in patients testing positive using RAPIRUN-HS. In conclusion, RAPIRUN-HS may be useful for determining whether to admit patients into hospitals and selecting the appropriate antimicrobial agents.

  17. Cirrus heterogeneity effects on cloud optical properties retrieved with an optimal estimation method from MODIS VIS to TIR channels

    NASA Astrophysics Data System (ADS)

    Fauchez, T.; Platnick, S.; Sourdeval, O.; Meyer, K.; Cornet, C.; Zhang, Z.; Szczap, F.

    2017-02-01

    This study presents preliminary results on the effect of cirrus heterogeneities on top-of-atmosphere (TOA) simulated radiances or reflectances for MODIS channels centered at 0.86, 2.21, 8.56, 11.01 and 12.03 µm, and on cloud optical properties retrieved with a research-level optimal estimation method (OEM). Synthetic cirrus cloud fields are generated using a 3D cloud generator (3DCLOUD) and radiances/reflectances are simulated using a 3D radiative transfer code (3DMCPOL). We find significant differences between the heterogeneity effects on either visible and near-infrared (VNIR) or thermal infrared (TIR) radiances. However, when both wavelength ranges are combined, heterogeneity effects are dominated by the VNIR horizontal radiative transport effect. As a result, small optical thicknesses are overestimated and large ones are underestimated. Retrieved effective diameter are found to be slightly affected, contrarily to retrievals using TIR channels only.

  18. Performance Evaluation of Machine Learning Methods for Leaf Area Index Retrieval from Time-Series MODIS Reflectance Data

    PubMed Central

    Wang, Tongtong; Xiao, Zhiqiang; Liu, Zhigang

    2017-01-01

    Leaf area index (LAI) is an important biophysical parameter and the retrieval of LAI from remote sensing data is the only feasible method for generating LAI products at regional and global scales. However, most LAI retrieval methods use satellite observations at a specific time to retrieve LAI. Because of the impacts of clouds and aerosols, the LAI products generated by these methods are spatially incomplete and temporally discontinuous, and thus they cannot meet the needs of practical applications. To generate high-quality LAI products, four machine learning algorithms, including back-propagation neutral network (BPNN), radial basis function networks (RBFNs), general regression neutral networks (GRNNs), and multi-output support vector regression (MSVR) are proposed to retrieve LAI from time-series Moderate Resolution Imaging Spectroradiometer (MODIS) reflectance data in this study and performance of these machine learning algorithms is evaluated. The results demonstrated that GRNNs, RBFNs, and MSVR exhibited low sensitivity to training sample size, whereas BPNN had high sensitivity. The four algorithms performed slightly better with red, near infrared (NIR), and short wave infrared (SWIR) bands than red and NIR bands, and the results were significantly better than those obtained using single band reflectance data (red or NIR). Regardless of band composition, GRNNs performed better than the other three methods. Among the four algorithms, BPNN required the least training time, whereas MSVR needed the most for any sample size. PMID:28045443

  19. Performance Evaluation of Machine Learning Methods for Leaf Area Index Retrieval from Time-Series MODIS Reflectance Data.

    PubMed

    Wang, Tongtong; Xiao, Zhiqiang; Liu, Zhigang

    2017-01-01

    Leaf area index (LAI) is an important biophysical parameter and the retrieval of LAI from remote sensing data is the only feasible method for generating LAI products at regional and global scales. However, most LAI retrieval methods use satellite observations at a specific time to retrieve LAI. Because of the impacts of clouds and aerosols, the LAI products generated by these methods are spatially incomplete and temporally discontinuous, and thus they cannot meet the needs of practical applications. To generate high-quality LAI products, four machine learning algorithms, including back-propagation neutral network (BPNN), radial basis function networks (RBFNs), general regression neutral networks (GRNNs), and multi-output support vector regression (MSVR) are proposed to retrieve LAI from time-series Moderate Resolution Imaging Spectroradiometer (MODIS) reflectance data in this study and performance of these machine learning algorithms is evaluated. The results demonstrated that GRNNs, RBFNs, and MSVR exhibited low sensitivity to training sample size, whereas BPNN had high sensitivity. The four algorithms performed slightly better with red, near infrared (NIR), and short wave infrared (SWIR) bands than red and NIR bands, and the results were significantly better than those obtained using single band reflectance data (red or NIR). Regardless of band composition, GRNNs performed better than the other three methods. Among the four algorithms, BPNN required the least training time, whereas MSVR needed the most for any sample size.

  20. Toxoplasma gondii from liquid nitrogen for continuous cell culture: methods to maximise efficient retrieval.

    PubMed

    Mavin, S; Evans, R; Chatterton, J M W; Ashburn, D; Joss, A W L; Ho-Yen, D O

    2003-01-01

    This study aims to increase the efficiency of continuous growth of Toxoplasma gondii in HeLa cells from tachyzoite stocks frozen in liquid nitrogen. Freezing and retrieval of tachyzoites for continuous cell culture requires more stringent protocols than those published for animal culture. The freezing and retrieval conditions are optimised so that a quality harvest (> or = 1 x 10(6) tachyzoites/mL, > or = 90% viability) can be produced using T. gondii recovered from liquid nitrogen as fast and reliably as possible. Retrieval success rate increased from 36% to 100%. An improved freezing procedure using chilled reagents and freshly harvested parasites, and adoption of an effective recovery protocol with retrieval of 3 x 10(7) tachyzoites into 75 cm2 flasks, change of maintenance media after six hours and subsequent blind passage all contributed to this success. The result is faster and more dependable production of T. gondii for diagnostic and experimental use.

  1. Toxoplasma gondii from liquid nitrogen for continuous cell culture:methods to maximise efficient retrieval.

    PubMed

    Mavin, S; Evans, R; Chatterton, J M W; Ashburn, D; Joss, A W L; Ho-Yen, D O

    2003-01-01

    This study aims to increase the efficiency of continuous growth of Toxoplasma gondii in HeLa cells from tachyzoite stocks frozen in liquid nitrogen. Freezing and retrieval of tachyzoites for continuous cell culture requires more stringent protocols than those published for animal culture. The freezing and retrieval conditions are optimised so that a quality harvest (≥ 1 x 10(6) tachyzoites/mL, ≥ 90% viability) can be produced using T. gondii recovered from liquid nitrogen as fast and reliably as possible. Retrieval success rate increased from 36% to 100%. An improved freezing procedure using chilled reagents and freshly harvested parasites, and adoption of an effective recovery protocol with retrieval of 3 x 10(7) tachyzoites into 75cm(2) flasks, change of maintenance media after six hours and subsequent blind passage all contributed to this success. The result is faster and more dependable production of T. gondii for diagnostic and experimental use.

  2. A simple and reliable immunohistochemical method for colocalization of 2 antigens in the same cells of paraffin-embedded tissues.

    PubMed

    Yan, Jun; Catts, Vibeke S; Chan, Anthony; McCombe, Pamela A

    2013-10-01

    Immunohistochemistry (IHC) lacks an efficient technique for colocalizing multiple antigens in the same cells of a single tissue section. The development of a methodology which combines the advantage of low cost, high sensitivity, and specificity would benefit clinical diagnosis and general research. On the basis of a newly published method of visualizing 2 antigens on a single paraffin-embedded tissue section, we have further developed a novel sequential technique for colocalizing 2 different antigens in a same cell in a paraffin-embedded tissue section. In this technique, we combined the microwave heating technique (MVT) with normal IHC methods to sequentially double stain a paraffin section; and colocalize 2 antigens in a single cell through result comparison stored in a digital management system. This MVT colocalization method has a higher degree of sensitivity and specificity comparable with conventional staining of both immunofluorescence and IHC systems. The primary advantage of this method is that it is inexpensive and convenient; the antibody(s) used in this method can be generated from the same or different species; it allows colocalization or comparison of different results of cell morphology for any single cell of the section on 2 images, avoids uncertainty when overlapping 2 antigens on a single image.

  3. Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method

    PubMed Central

    Khajuria, Nidhi; Mamta; Ramesh, Gayathri

    2016-01-01

    Introduction The ABO blood group system was the significant element for forensic serological examination of blood and body fluids in the past before the wide adaptation of DNA typing. A significant proportion of individuals (80%) are secretors, meaning that antigens present in the blood are also found in other body fluids such as saliva. Absorption inhibition is one such method that works by reducing strength of an antiserum based on type and amount of antigen present in the stains. Aim To check the efficacy of identifying the blood group antigens in saliva and to know the secretor status using absorption inhibition method among southern Rajasthan population. Materials and Methods Blood and saliva samples were collected from 80 individuals comprising 20 individuals in each blood group. The absorption inhibition method was used to determine the blood group antigens in the saliva and then the results were correlated with the blood group of the collected blood sample. The compiled data was statistically analysed using chi-square test. Results Blood groups A & O revealed 100% secretor status for both males and females. While blood groups B and AB revealed 95% secretor status. Conclusion Secretor status evaluation of the ABO blood group antigen in saliva using absorption inhibition method can be a useful tool in forensic examination. PMID:27042574

  4. Three methods to retrieve slant total electron content measurements from ground-based GPS receivers and performance assessment

    NASA Astrophysics Data System (ADS)

    Zhang, Baocheng

    2016-07-01

    The high sampling rate along with the global coverage of ground-based receivers makes Global Positioning System (GPS) data particularly ideal for sensing the Earth's ionosphere. Retrieval of slant total electron content measurements (TECMs) constitutes a key first step toward extracting various ionospheric parameters from GPS data. Within the ionospheric community, the interpretation of TECM is widely recognized as the slant total electron content along the satellite receiver line of sight, biased by satellite and receiver differential code biases (DCBs). The Carrier-to-Code Leveling (CCL) has long been used as a geometry-free method for retrieving TECM, mainly because of its simplicity and effectiveness. In fact, however, the CCL has proven inaccurate as it may give rise to TECM very susceptible to so-called leveling errors. With the goal of attaining more accurate TECM retrieval, we report in this contribution two other methods than the CCL, namely, the Precise Point Positioning (PPP) and the Array-aided PPP (A-PPP). The PPP further exploits the International GPS Service (IGS) orbit and clock products and turns out to be a geometry-based method. The A-PPP is designed to retrieve TECM from an array of colocated receivers, taking advantage of the broadcast orbit and clock products. Moreover, A-PPP also takes into account the fact that the ionospheric effects measured from one satellite to all colocated receivers ought to be the same, thus leading to the estimability of interreceiver DCB. We perform a comparative study of the formal precision and the empirical accuracy of the TECM that are retrieved, respectively, by three methods from the same set of GPS data. Results of such a study can be used to assess the actual performance of the three methods. In addition, we check the temporal stability in A-PPP-derived interreceiver DCB estimates over time periods ranging from 1 to 3 days.

  5. Connectionist Interaction Information Retrieval.

    ERIC Educational Resources Information Center

    Dominich, Sandor

    2003-01-01

    Discussion of connectionist views for adaptive clustering in information retrieval focuses on a connectionist clustering technique and activation spreading-based information retrieval model using the interaction information retrieval method. Presents theoretical as well as simulation results as regards computational complexity and includes…

  6. Utility of PCR, Culture, and Antigen Detection Methods for Diagnosis of Legionellosis

    PubMed Central

    Chen, Derrick J.; Procop, Gary W.; Vogel, Sherilynn; Yen-Lieberman, Belinda

    2015-01-01

    The goal of this retrospective study was to evaluate the performance of different diagnostic tests for Legionnaires' disease in a clinical setting where Legionella pneumophila PCR had been introduced. Electronic medical records at the Cleveland Clinic were searched for Legionella urinary antigen (UAG), culture, and PCR tests ordered from March 2010 through December 2013. For cases where two or more test methods were performed and at least one was positive, the medical record was reviewed for relevant clinical and epidemiologic factors. Excluding repeat testing on a given patient, 19,912 tests were ordered (12,569 UAG, 3,747 cultures, and 3,596 PCR) with 378 positive results. The positivity rate for each method was 0.4% for culture, 0.8% for PCR, and 2.7% for UAG. For 37 patients, at least two test methods were performed with at least one positive result: 10 (27%) cases were positive by all three methods, 16 (43%) were positive by two methods, and 11 (30%) were positive by one method only. For the 32 patients with medical records available, clinical presentation was consistent with proven or probable Legionella infection in 84% of the cases. For those cases, the sensitivities of culture, PCR, and UAG were 50%, 92%, and 96%, respectively. The specificities were 100% for culture and 99.9% for PCR and UAG. PMID:26292304

  7. To Clone or Not To Clone: Method Analysis for Retrieving Consensus Sequences In Ancient DNA Samples

    PubMed Central

    Winters, Misa; Barta, Jodi Lynn; Monroe, Cara; Kemp, Brian M.

    2011-01-01

    The challenges associated with the retrieval and authentication of ancient DNA (aDNA) evidence are principally due to post-mortem damage which makes ancient samples particularly prone to contamination from “modern” DNA sources. The necessity for authentication of results has led many aDNA researchers to adopt methods considered to be “gold standards” in the field, including cloning aDNA amplicons as opposed to directly sequencing them. However, no standardized protocol has emerged regarding the necessary number of clones to sequence, how a consensus sequence is most appropriately derived, or how results should be reported in the literature. In addition, there has been no systematic demonstration of the degree to which direct sequences are affected by damage or whether direct sequencing would provide disparate results from a consensus of clones. To address this issue, a comparative study was designed to examine both cloned and direct sequences amplified from ∼3,500 year-old ancient northern fur seal DNA extracts. Majority rules and the Consensus Confidence Program were used to generate consensus sequences for each individual from the cloned sequences, which exhibited damage at 31 of 139 base pairs across all clones. In no instance did the consensus of clones differ from the direct sequence. This study demonstrates that, when appropriate, cloning need not be the default method, but instead, should be used as a measure of authentication on a case-by-case basis, especially when this practice adds time and cost to studies where it may be superfluous. PMID:21738625

  8. A Novel Method to Assess Wear Rates of Retrieved Tibial Inserts Following in-vivo Use

    NASA Astrophysics Data System (ADS)

    Paniogue, Tanille J.

    Ultra-high molecular weight polyethylene (UHMWPE) on cobalt chrome is the bearing couple of choice for total knee arthroplasty. The number of patients undergoing total knee arthroplasty has been steadily growing and is projected to continue increasing rapidly in the near future. Many of these patients are younger and more active and therefore need a longer lasting device. However, many of these devices fail prematurely and often the primary reason for failure and ultimately revision is due to wear related issues. Therefore, examining how wear rates of the UHMWPE tibial insert change during in-vivo use can help elucidate the mechanisms of accelerated wear and hopefully aid in finding solutions to combat wear related failures. Different crosslinking treatments have been employed by manufacturers to improve wear resistance of the polyethylene. While this has been shown to be an effective way to reduce wear, crosslinking has led to other issues such as oxidative instability and a decline in mechanical properties. The purpose of this body of work is to examine how changes in oxidation, after in-vivo use, affect wear resistance. A novel testing method was developed to test the native articular surface from retrieved tibial inserts in a laboratory Pin-on-Disk (POD) simulator. The method was validated using short-duration implant articular surfaces and non-articular control pins. In the absence of high surface oxidation or severe surface damage, the articular surface pins had comparable steady state wear rates to their bulk counterparts. Tests of devices with longer in-vivo service show chemical changes consistent with a free-radical mediated oxidation mechanism. Tribological assessment of the articular surfaces shows increasing wear rates as a function of oxidation. While this relationship has been hypothesized in the literature, these experiments represent the first physical demonstration of the phenomenon. The wear mechanism is further explored through infrared

  9. New method for simultaneous gas and aerosol retrievals from space limb-scanning spectral observation of the atmosphere.

    PubMed

    Oshchepkov, Sergey; Sasano, Yasuhiro; Yokota, Tatsuya

    2002-07-20

    This study concerns the development of a new inversion method for simultaneous gas and aerosol retrievals in the upper layers of the atmosphere from limb-viewing multiwavelength-transmission infrared measurements. In this method, concentrations of gas species such as O3, NO2, HNO3, N2O, CH4, and H2O, and spectral dependences of the aerosol extinction coefficient are retrieved simultaneously. When this is done, smoothness constraints on the desired spectral dependencies of the aerosol extinction coefficient are used as an a priori assumption. The method is used in the treating of synthetic transmission spectra of the Improved Limb Atmospheric Spectrometer, which is based on the solar occultation technique and was on board the Advanced Earth Observing Satellite. A set of numerical tests shows the efficiency of the method.

  10. Retrieving microphysical properties and air motion of cirrus clouds based on the doppler moments method using cloud radar

    NASA Astrophysics Data System (ADS)

    Zhong, Lingzhi; Liu, Liping; Deng, Min; Zhou, Xiuji

    2012-05-01

    Radar parameters including radar reflectivity, Doppler velocity, and Doppler spectrum width were obtained from Doppler spectrum moments. The Doppler spectrum moment is the convolution of both the particle spectrum and the mean air vertical motion. Unlike strong precipitation, the motion of particles in cirrus clouds is quite close to the air motion around them. In this study, a method of Doppler moments was developed and used to retrieve cirrus cloud microphysical properties such as the mean air vertical velocity, mass-weighted diameter, effective particle size, and ice content. Ice content values were retrieved using both the Doppler spectrum method and classic Z-IWC (radar reflectivity-ice water content) relationships; however, the former is a more reasonable method.

  11. Coherent diffractive imaging beyond the Fresnel approximation using a deterministic phase-retrieval method with an aperture-array filter.

    PubMed

    Nakajima, Nobuharu

    2013-03-01

    Previously, we have proposed a lensless coherent imaging using a nonholographic and noniterative phase-retrieval method that allows the reconstruction of a complex-valued object from a single diffraction intensity measured with an aperture-array filter. The proof-of-concept experiment of this method has been demonstrated under the Fresnel diffraction approximation. In applications to microscopy, however, the measurement of the diffraction intensity with high numerical aperture beyond the Fresnel approximation is required to obtain the object information at high spatial resolution. Thus we have also presented an extension procedure to apply the method to the cases beyond the Fresnel approximation by means of computer simulations. Here the effectiveness of the procedure is demonstrated by the experiments, in which the reconstruction with about 10 times the resolution of our previous experiment has been achieved and the object information in depth direction has been retrieved.

  12. CODA METHOD AT HIGH FREQUENCIES: RETRIEVING SOURCE PARAMETERS OF SMALL (M1-M2) EARTHQUAKES

    NASA Astrophysics Data System (ADS)

    Viegas, G.; Abercrombie, R. E.; Mayeda, K. M.

    2009-12-01

    We calculate source parameters of small earthquakes from coda derived source spectrum ratios, extending the coda method to higher frequencies and smaller crustal volumes. To validate our high frequency results, we compare our source parameters estimates to the ones obtained with a direct wave study for the same set of earthquakes. We investigate earthquake source scaling relationships using local and regional good quality recordings of the M5 2002 Au Sable Forks, NY, mainshock and aftershocks sequence (M4-M1), and regional recordings of other moderate Eastern North America (ENA) earthquakes. We successfully retrieve spectral ratios, with a clear corner frequency and omega squared fall off, for the small (M2) Au Sable Forks aftershocks recorded locally (~3 km to 12 km epicentral distances) at a high sampling rate (200 sps). The local data have maximum coda duration of 15 s after the S wave onset (triggered recording). We investigate if the records are long enough, by first testing the method with Parkfield, CA, earthquakes, a dataset with similar dimensions in terms of station epicentral distances, earthquake magnitudes, and instrument sampling rate, for which records with long codas are available. The method holds for both datasets at high frequencies (up to 80 Hz), with a small increase of inter-station coda amplitude instability (0.5 standard deviation), expected for smaller earthquakes. We obtain in average higher corner frequencies and stress drops estimates (factor of 1.7 for corner frequency and 4.3 for stress drop) for the small (~M2) locally recorded Au Sable Forks earthquakes, than the values obtained with a direct wave study, and in average lower corner frequencies and stress drops for the moderate regionally recorded earthquakes (factor of 0.7 for corner frequency and 0.4 for stress drop). The corner frequencies of the mainshock and M3 earthquakes are close to the usable band limit and so uncertainties in the estimates of corner frequency and stress drop

  13. Applying Information-Retrieval Methods to Software Reuse: A Case Study.

    ERIC Educational Resources Information Center

    Stierna, Eric J.; Rowe, Neil C.

    2003-01-01

    Discusses reuse of existing software for new purposes as a key aspect of efficient software engineering by matching formal written requirements used to define the new and the old software. Explores two matching methodologies that use information retrieval techniques and describes test results from a comparison of two military systems. (Author/LRW)

  14. User-Centered Perspective of Information Retrieval Research and Analysis Methods.

    ERIC Educational Resources Information Center

    Sugar, William

    1995-01-01

    Reviews information retrieval (IR) studies since 1986 from the user's perspective. Identifies two main approaches that advocate user-centered design theory: (1) the cognitive approach; and (2) the holistic approach. Also explores other approaches--systems thinking/action research and usability techniques that may have potential for IR research and…

  15. A simple method for retrieving significant wave height from Dopplerized X-band radar

    NASA Astrophysics Data System (ADS)

    Carrasco, Ruben; Streßer, Michael; Horstmann, Jochen

    2017-02-01

    Retrieving spectral wave parameters such as the peak wave direction and wave period from marine radar backscatter intensity is very well developed. However, the retrieval of significant wave height is difficult because the radar image spectrum (a backscatter intensity variance spectrum) has to be transferred to a wave spectrum (a surface elevation variance spectrum) using a modulation transfer function (MTF) which requires extensive calibration for each individual radar setup. In contrast to the backscatter intensity, the Doppler velocity measured by a coherent radar is induced by the radial velocity (or line-of-sight velocity) of the surface scattering and its periodic component is mainly the contribution of surface waves. Therefore, the variance of the Doppler velocity can be utilized to retrieve the significant wave height. Analyzing approximately 100 days of Doppler velocity measurements of a coherent-on-receive radar operating at X-band with vertical polarization in transmit and receive, a simple relation was derived and validated to retrieve significant wave heights. Comparison to wave measurements of a wave rider buoy as well as an acoustic wave and current profiler resulted in a root mean square error of 0.24 m with a bias of 0.08 m. Furthermore, the different sources of error are discussed and investigated.

  16. Anti-noise algorithm of lidar data retrieval by combining the ensemble Kalman filter and the Fernald method.

    PubMed

    Mao, Feiyue; Gong, Wei; Li, Chen

    2013-04-08

    The lidar signal-to-noise ratio decreases rapidly with an increase in range, which severely affects the retrieval accuracy and the effective measure range of a lidar based on the Fernald method. To avoid this issue, an alternative approach is proposed to simultaneously retrieve lidar data accurately and obtain a de-noised signal as a by-product by combining the ensemble Kalman filter and the Fernald method. The dynamical model of the new algorithm is generated according to the lidar equation to forecast backscatter coefficients. In this paper, we use the ensemble sizes as 60 and the factor δ(1/2) as 1.2 after being weighed against the accuracy and the time cost based on the performance function we define. The retrieval and de-noising results of both simulated and real signals demonstrate that our method is practical and effective. An extensive application of our method can be useful for the long-term determining of the aerosol optical properties.

  17. Retrieval of diffusing surface by two-frame interferometric method with blind phase shift of a reference wave

    NASA Astrophysics Data System (ADS)

    Muravsky, Leonid I.; Kmet', Arkady B.; Voronyak, Taras I.

    2011-08-01

    Two-frame interferometric method with blind phase shift of a reference wave for smooth surfaces retrieval is considered. The ability of this method to reconstruct a macrorelief of diffusing surfaces with a given roughness is studied. Computer simulations have testified the ability of reliable low-noise reconstruction of the diffusing surface macrorelief with standard deviation of the roughness heights up to λ/10 by using the developed interferogram processing algorithm. The simulations have shown that the proposed correlation approach, which is used to determine the reference wave blind phase shift, is more suitable for a diffusing surface than for a smooth one and the increase of surface roughness leads to a quadruple decrease of this error in comparison with that for the smooth surface. Experimental verification of the interferometric method performance to retrieve real diffusing surface macroreliefs with given roughness has been done by using the experimental setup based on a Twyman-Green interferometer and roughness comparison specimen. The obtained experimental results virtually have coincided with the computer simulation results that prove the performance of the considered method to retrieve not only smooth, but also diffusing surfaces.

  18. A new method for the evaluation of antibody affinity based on serial dilutions of studied antibody-antigen mixture.

    PubMed

    Bobrovnik, S A

    2002-01-01

    A new method for the evaluation of the affinity of bivalent antibodies were suggested. This method is based on the previously published by the author the idea of using so-called coordinates of dilutions. It was shown that the suggested method allows to evaluate the affinity of antibodies with high accuracy using this simple approach. It is supposed that at some conditions the suggested method could have substantial advantages in comparison to the traditional methods. This method allows to analyze situations when antibodies are already in a mixture with antigen, for example in the bloodstream in the case of infections or autoimmune diseases. The method provides useful approach for the evaluation not only antibody affinity, but also the concentration of circulated antigen.

  19. Method for utilizing properties of the sinc(x) function for phase retrieval on nyquist-under-sampled data

    NASA Technical Reports Server (NTRS)

    Dean, Bruce H. (Inventor); Smith, Jeffrey Scott (Inventor); Aronstein, David L. (Inventor)

    2012-01-01

    Disclosed herein are systems, methods, and non-transitory computer-readable storage media for simulating propagation of an electromagnetic field, performing phase retrieval, or sampling a band-limited function. A system practicing the method generates transformed data using a discrete Fourier transform which samples a band-limited function f(x) without interpolating or modifying received data associated with the function f(x), wherein an interval between repeated copies in a periodic extension of the function f(x) obtained from the discrete Fourier transform is associated with a sampling ratio Q, defined as a ratio of a sampling frequency to a band-limited frequency, and wherein Q is assigned a value between 1 and 2 such that substantially no aliasing occurs in the transformed data, and retrieves a phase in the received data based on the transformed data, wherein the phase is used as feedback to an optical system.

  20. An inversion method for retrieving soil moisture information from satellite altimetry observations

    NASA Astrophysics Data System (ADS)

    Uebbing, Bernd; Forootan, Ehsan; Kusche, Jürgen; Braakmann-Folgmann, Anne

    2016-04-01

    Soil moisture represents an important component of the terrestrial water cycle that controls., evapotranspiration and vegetation growth. Consequently, knowledge on soil moisture variability is essential to understand the interactions between land and atmosphere. Yet, terrestrial measurements are sparse and their information content is limited due to the large spatial variability of soil moisture. Therefore, over the last two decades, several active and passive radar and satellite missions such as ERS/SCAT, AMSR, SMOS or SMAP have been providing backscatter information that can be used to estimate surface conditions including soil moisture which is proportional to the dielectric constant of the upper (few cm) soil layers . Another source of soil moisture information are satellite radar altimeters, originally designed to measure sea surface height over the oceans. Measurements of Jason-1/2 (Ku- and C-Band) or Envisat (Ku- and S-Band) nadir radar backscatter provide high-resolution along-track information (~ 300m along-track resolution) on backscatter every ~10 days (Jason-1/2) or ~35 days (Envisat). Recent studies found good correlation between backscatter and soil moisture in upper layers, especially in arid and semi-arid regions, indicating the potential of satellite altimetry both to reconstruct and to monitor soil moisture variability. However, measuring soil moisture using altimetry has some drawbacks that include: (1) the noisy behavior of the altimetry-derived backscatter (due to e.g., existence of surface water in the radar foot-print), (2) the strong assumptions for converting altimetry backscatters to the soil moisture storage changes, and (3) the need for interpolating between the tracks. In this study, we suggest a new inversion framework that allows to retrieve soil moisture information from along-track Jason-2 and Envisat satellite altimetry data, and we test this scheme over the Australian arid and semi-arid regions. Our method consists of: (i

  1. A Rapid and Sensitive Method for the Quantitation of Legionella Pneumophila Antigen from Human Urine.

    DTIC Science & Technology

    1980-11-12

    reverse% passive hemagglutination test was developed to assay concentrations of solubl4 antigen of Legionnaires ’ Disease ( Legionella pneumophila) in...reversed passive hemagglutination test was developed to assay concentrations of soluble antigen of Legionnaires disease ( Legionella pneumophila) in...G. W. Gorman, R. E. Weaver, D. C. Mackel, and H. W. Smith. 1978. Primary isolation media for Legionnaires ’ disease bacterium. J. Clin. Hicrobiol. 8

  2. A single field of view method for retrieving tropospheric temperature profiles from cloud-contaminated radiance data

    NASA Technical Reports Server (NTRS)

    Hodges, D. B.

    1976-01-01

    An iterative method is presented to retrieve single field of view (FOV) tropospheric temperature profiles directly from cloud-contaminated radiance data. A well-defined temperature profile may be calculated from the radiative transfer equation (RTE) for a partly cloudy atmosphere when the average fractional cloud amount and cloud-top height for the FOV are known. A cloud model is formulated to calculate the fractional cloud amount from an estimated cloud-top height. The method is then examined through use of simulated radiance data calculated through vertical integration of the RTE for a partly cloudy atmosphere using known values of cloud-top height(s) and fractional cloud amount(s). Temperature profiles are retrieved from the simulated data assuming various errors in the cloud parameters. Temperature profiles are retrieved from NOAA-4 satellite-measured radiance data obtained over an area dominated by an active cold front and with considerable cloud cover and compared with radiosonde data. The effects of using various guessed profiles and the number of iterations are considered.

  3. A novel method for evaluating natural and vaccine induced serological responses to Bordetella pertussis antigens.

    PubMed

    Berbers, G A M; van de Wetering, M S E; van Gageldonk, P G M; Schellekens, J F P; Versteegh, F G A; Teunis, P F M

    2013-08-12

    We studied the time course of serum IgG antibodies against 3 different pertussis vaccine antigens: PT (pertussis toxin), FHA (filamentous hemagglutinin), Prn (pertactin) in sera from individuals vaccinated with four different pertussis vaccines at 4 years of age: (N=44, 44, 23 and 23, respectively,) and compared the responses to/after natural infection with Bordetella pertussis (N=44, age 1-8 years). These longitudinal data were analyzed with a novel method, using a mathematical model to describe the observed responses, and their variation among subjects. This allowed us to estimate biologically meaningful characteristics of the serum antibody response, like peak level and decay rate, and to compare these among natural infections and vaccine responses. Compared to natural infection, responses to PT after vaccination with the tested vaccines are smaller in magnitude and tend to decay slightly faster. When present in vaccines, FHA and Prn tend to produce high peak levels, higher than those in naturally infected patients, but these decay faster. As expected, the Dutch whole cell vaccine produced lower antibody responses than the acellular vaccines. This model allows a better comparison of the kinetics of vaccine induced antibody responses and after natural infection over a long follow up period.

  4. A novel method to identify and characterise peptide mimotopes of heat shock protein 70-associated antigens.

    PubMed

    Arnaiz, Blanca; Madrigal-Estebas, Laura; Todryk, Stephen; James, Tharappel C; Doherty, Derek G; Bond, Ursula

    2006-04-08

    The heat shock protein, Hsp70, has been shown to play an important role in tumour immunity. Vaccination with Hsp70-peptide complexes (Hsp70-PCs), isolated from autologous tumour cells, can induce protective immune responses. We have developed a novel method to identify synthetic mimic peptides of Hsp70-PCs and to test their ability to activate T-cells. Peptides (referred to as "recognisers") that bind to Hsp70-PCs from the human breast carcinoma cell line, MDA-MB-231, were identified by bio-panning a random peptide M13 phage display library. Synthetic recogniser peptides were subsequently used as bait in a reverse bio-panning experiment to identify potential Hsp70-PC mimic peptides. The ability of the recogniser and mimic peptides to prime human lymphocyte responses against tumour cell antigens was tested by stimulating lymphocytes with autologous peptide-loaded monocyte-derived dendritic cells (DCs). Priming and subsequent stimulation with either the recogniser or mimic peptide resulted in interferon-gamma (IFN-gamma) secretion by the lymphocytes. Furthermore, DCs loaded with Hsp70, Hsp70-PC or the recogniser or the mimic peptide primed the lymphocytes to respond to soluble extracts from breast cells. These results highlight the potential application of synthetic peptide-mimics of Hsp70-PCs, as modulators of the immune response against tumours.

  5. Nonadherent culture method downregulates stem cell antigen-1 expression in mouse bone marrow mesenchymal stem cells

    PubMed Central

    DENG, BAOPING; DENG, WEIPING; XIAO, PINGNAN; ZENG, KUAN; ZHANG, SHINING; ZHANG, HONGWU; DENG, DAVID YB; YANG, YANQI

    2015-01-01

    Mesenchymal stem cells (MSCs) are primarily isolated by their adherence to plastic and their in vitro growth characteristics. Expansion of these cells from an adherent culture is the only method to obtain a sufficient number of cells for use in clinical practice and research. However, little is known with regard to the effect of adherence to plastic on the phenotype of the cells. In the present study, bone marrow CD45−CD31−CD44− stem cell antigen (Sca)-1+ MSCs were sorted by flow cytometry and expanded in adherent cultures. The expression levels of the adhesion molecule, Sca-1, in the adherent cultures were compared with those from nonadherent cultures at different time points. The flow cytometry results indicated that the expression levels of Sca-1 decreased in the MSCs in the nonadherent cultures grown in ultra-low-adherent plates. Furthermore, the result was confirmed by quantitative polymerase chain reaction at the same time points. Therefore, the results demonstrated that the loss of plastic adherence downregulated the expression of Sca-1. The observations may provide novel insights into the molecular mechanisms underlying plastic adherent culture. PMID:26170908

  6. De-noising and retrieving algorithm of Mie lidar data based on the particle filter and the Fernald method.

    PubMed

    Li, Chen; Pan, Zengxin; Mao, Feiyue; Gong, Wei; Chen, Shihua; Min, Qilong

    2015-10-05

    The signal-to-noise ratio (SNR) of an atmospheric lidar decreases rapidly as range increases, so that maintaining high accuracy when retrieving lidar data at the far end is difficult. To avoid this problem, many de-noising algorithms have been developed; in particular, an effective de-noising algorithm has been proposed to simultaneously retrieve lidar data and obtain a de-noised signal by combining the ensemble Kalman filter (EnKF) and the Fernald method. This algorithm enhances the retrieval accuracy and effective measure range of a lidar based on the Fernald method, but sometimes leads to a shift (bias) in the near range as a result of the over-smoothing caused by the EnKF. This study proposes a new scheme that avoids this phenomenon using a particle filter (PF) instead of the EnKF in the de-noising algorithm. Synthetic experiments show that the PF performs better than the EnKF and Fernald methods. The root mean square error of PF are 52.55% and 38.14% of that of the Fernald and EnKF methods, and PF increases the SNR by 44.36% and 11.57% of that of the Fernald and EnKF methods, respectively. For experiments with real signals, the relative bias of the EnKF is 5.72%, which is reduced to 2.15% by the PF in the near range. Furthermore, the suppression impact on the random noise in the far range is also made significant via the PF. An extensive application of the PF method can be useful in determining the local and global properties of aerosols.

  7. Epstein-Barr virus viral capsid antigen titer by immunofluorescence with microplates: new semiautomated method based on the microtiter system.

    PubMed Central

    Lamy, M E; Favart, A M; Burtonboy, G; Arana, A

    1977-01-01

    A semiautomated method of an indirect immunofluorescence technique for the titration of antibodies directed against viral capsid antigens of Epstein-Barr virus has been developed with Microtiter system units. By this method, a technician is able to titrate some hundred samples daily. The technique is safe, easy, and reproducible. The various procedures are described, and the sensitivity of the test is discussed. Images PMID:195981

  8. Information storage medium and method of recording and retrieving information thereon

    DOEpatents

    Marchant, D. D.; Begej, Stefan

    1986-01-01

    Information storage medium comprising a semiconductor doped with first and second impurities or dopants. Preferably, one of the impurities is introduced by ion implantation. Conductive electrodes are photolithographically formed on the surface of the medium. Information is recorded on the medium by selectively applying a focused laser beam to discrete regions of the medium surface so as to anneal discrete regions of the medium containing lattice defects introduced by the ion-implanted impurity. Information is retrieved from the storage medium by applying a focused laser beam to annealed and non-annealed regions so as to produce a photovoltaic signal at each region.

  9. New Term Weighting Formulas for the Vector Space Method in Information Retrieval

    SciTech Connect

    Chisholm, E.; Kolda, T.G.

    1999-03-01

    The goal in information retrieval is to enable users to automatically and accurately find data relevant to their queries. One possible approach to this problem i use the vector space model, which models documents and queries as vectors in the term space. The components of the vectors are determined by the term weighting scheme, a function of the frequencies of the terms in the document or query as well as throughout the collection. We discuss popular term weighting schemes and present several new schemes that offer improved performance.

  10. Enzyme-labeled Antigen Method: Development and Application of the Novel Approach for Identifying Plasma Cells Locally Producing Disease-specific Antibodies in Inflammatory Lesions

    PubMed Central

    Mizutani, Yasuyoshi; Shiogama, Kazuya; Onouchi, Takanori; Sakurai, Kouhei; Inada, Ken-ichi; Tsutsumi, Yutaka

    2016-01-01

    In chronic inflammatory lesions of autoimmune and infectious diseases, plasma cells are frequently observed. Antigens recognized by antibodies produced by the plasma cells mostly remain unclear. A new technique identifying these corresponding antigens may give us a breakthrough for understanding the disease from a pathophysiological viewpoint, simply because the immunocytes are seen within the lesion. We have developed an enzyme-labeled antigen method for microscopic identification of the antigen recognized by specific antibodies locally produced in plasma cells in inflammatory lesions. Firstly, target biotinylated antigens were constructed by the wheat germ cell-free protein synthesis system or through chemical biotinylation. Next, proteins reactive to antibodies in tissue extracts were screened and antibody titers were evaluated by the AlphaScreen method. Finally, with the enzyme-labeled antigen method using the biotinylated antigens as probes, plasma cells producing specific antibodies were microscopically localized in fixed frozen sections. Our novel approach visualized tissue plasma cells that produced 1) autoantibodies in rheumatoid arthritis, 2) antibodies against major antigens of Porphyromonas gingivalis in periodontitis or radicular cyst, and 3) antibodies against a carbohydrate antigen, Strep A, of Streptococcus pyogenes in recurrent tonsillitis. Evaluation of local specific antibody responses expectedly contributes to clarifying previously unknown processes in inflammatory disorders. PMID:27006517

  11. Novel methods for expression of foreign antigens in live vector vaccines

    PubMed Central

    Wang, Jin Yuan; Harley, Regina H.; Galen, James E.

    2013-01-01

    Bacterial live vector vaccines represent a vaccine development strategy that offers exceptional flexibility. In this approach, genes encoding protective antigens of unrelated bacterial, viral or parasitic pathogens are expressed in an attenuated bacterial vaccine strain that delivers these foreign antigens to the immune system, thereby eliciting relevant immune responses. Rather than expressing these antigens using low copy expression plasmids, here we pursue expression of foreign proteins from the live vector chromosome. Our strategy is designed to compensate for the inherent disadvantage of loss of gene dosage (vs. plasmid-based expression) by integrating antigen-encoding gene cassettes into multiple chromosomal sites already inactivated in an attenuated Salmonella enterica serovar Typhi vaccine candidate. We tested expression of a cassette encoding the green fluorescent protein (GFPuv) integrated separately into native guaBA, htrA or clyA chromosomal loci. Using single integrations, we show that expression levels of GFPuv are significantly affected by the site of integration, regardless of the inclusion of additional strong promoters within the incoming cassette. Using cassettes integrated into both guaBA and htrA, we observe cumulative synthesis levels from two integration sites superior to single integrations. Most importantly, we observe that GFPuv expression increases in a growth phase-dependent manner, suggesting that foreign antigen synthesis may be “tuned” to the physiology of the live vaccine. We expect this novel platform expression technology to prove invaluable in the development of a wide variety of multivalent live vector vaccines, capable of expressing multiple antigens from both chromosomal and plasmid-based expression systems within a single strain. PMID:23406777

  12. [Detection and quantification of weak concentrations of antigens using a sensitive and direct method of demonstrating immunoprecipitation reactions].

    PubMed

    Rochu, D; Crespeau, H; Fine, A; Marneux, M; Fine, J M

    1989-09-01

    Using agarose gel coated on GelBond film sheets, and using Coomassie blue stain followed by silver stain, a sensitive microassay has been developed for detecting small amounts of antigen-antibody precipitates, and for quantitating low concentrations of antigen. In order to obtain a high sensitivity, antigen-antibody ratios were adjusted imperatively close to the equivalence in double-diffusion, and for quantitative estimation, single radial immunodiffusion was performed, according to Mancini, by measuring circles at the end point. The use of a double staining procedure allows to detect as little as 8 micrograms/ml antigen by Ouchterlony and 200 ng/ml by Mancini technique. The sensitivity of the method is 100 times greater than classical techniques and other advantages such as the need for minimal amounts of unconcentrated samples, the absence of radioactive labelling, and the absence of interference due to a second or a third antibody coat, make this assay useful for analyzing and quantitating monoclonal antibodies obtained by hybridoma or B-cell immortalization.

  13. Soil Moisture Retrieval from Polarimetric SAR Data: A Short Review of Existing Methods and a New One

    NASA Astrophysics Data System (ADS)

    Di Martino, Gerardo; Iodice, Antonio; Poreh, Davod; Riccio, Daniele

    2016-08-01

    Soil moisture retrieval from SAR data is not an easy task, especially in presence of vegetation cover. Accordingly, in recent years several methods for soil-moisture retrieval under vegetation cover have been developed, relying on model-based or hybrid polarimetric target- decomposition techniques. However, most of these decomposition techniques suffer from the so-called negative-power problem, which is mainly related to poor modelling of surface- and/or volume-scattering contributions. In this paper we, first, analyse the Polarimetric Two-Scale Two-Component Model and the Iterative Generalized Hybrid Decomposition method, proposing a way to combine the estimation results of the methods so that most vegetation conditions can be accounted for. Then, we introduce a method that tries to solve the case of dominant surface scattering and non- negligible dihedral scattering, which is the case not covered by the abovementioned model combination. Meaningful estimation results are presented and discussed using polarimetric L-band SAR data of the AgriSAR 2006 campaign.

  14. An improved tropospheric ozone database retrieved from SCIAMACHY Limb-Nadir-Matching method

    NASA Astrophysics Data System (ADS)

    Jia, Jia; Rozanov, Alexei; Ladstätter-Weißenmayer, Annette; Ebojie, Felix; Rahpoe, Nabiz; Bötel, Stefan; Burrows, John

    2015-04-01

    Tropospheric ozone is one of the most important green-house gases and the main component of photochemical smog. It is either transported from the stratosphere or photochemically produced during pollution events in the troposphere that threaten the respiratory system. To investigate sources, transport mechanisms of tropospheric ozone in a global view, limb nadir matching (LNM) technique applied with SCIAMACHY instrument is used to retrieve tropospheric ozone. With the fact that 90% ozone is located in the stratosphere and only about 10% can be observed in the troposphere, the usage of satellite data requires highly qualified nadir and limb data. In this study we show an improvement of SCIAMACHY limb data as well as its influence on tropospheric ozone results. The limb nadir matching technique is also refined to increase the quality of the tropospheric ozone. The results are validated with ozone sonde measurements.

  15. Application of the Markov Chain Monte Carlo method for snow water equivalent retrieval based on passive microwave measurements

    NASA Astrophysics Data System (ADS)

    Pan, J.; Durand, M. T.; Vanderjagt, B. J.

    2015-12-01

    Markov Chain Monte Carlo (MCMC) method is a retrieval algorithm based on Bayes' rule, which starts from an initial state of snow/soil parameters, and updates it to a series of new states by comparing the posterior probability of simulated snow microwave signals before and after each time of random walk. It is a realization of the Bayes' rule, which gives an approximation to the probability of the snow/soil parameters in condition of the measured microwave TB signals at different bands. Although this method could solve all snow parameters including depth, density, snow grain size and temperature at the same time, it still needs prior information of these parameters for posterior probability calculation. How the priors will influence the SWE retrieval is a big concern. Therefore, in this paper at first, a sensitivity test will be carried out to study how accurate the snow emission models and how explicit the snow priors need to be to maintain the SWE error within certain amount. The synthetic TB simulated from the measured snow properties plus a 2-K observation error will be used for this purpose. It aims to provide a guidance on the MCMC application under different circumstances. Later, the method will be used for the snowpits at different sites, including Sodankyla, Finland, Churchill, Canada and Colorado, USA, using the measured TB from ground-based radiometers at different bands. Based on the previous work, the error in these practical cases will be studied, and the error sources will be separated and quantified.

  16. The use of measured RF power signals to evaluate feasibility of inverse methods to retrieve refractivity parameters

    NASA Astrophysics Data System (ADS)

    Gustafsson, Ove K. S.; Eriksson, Gunnar; Holm, Peter; Waern, Åsa; von Schoenberg, Pontus; Thaning, Lennart; Nordstrand, Melker; Persson, Rolf

    2006-09-01

    Radio wave propagation over sea paths is influenced by the local meteorological condition at the atmospheric layer near the surface, especially during ducts. Duct condition can be determined by measurements of local meteorological parameters, by weather forecast models or by using inverse methods. In order to evaluate the feasibility of using inverse methods to retrieve the refractivity profiles a measurement of RF signals and meteorological parameters were carried out at a test site in the Baltic. During the measurements, signal power from two broadcast antennas, one at Visby and one at Vastervik, were received at Musko, an island south of Stockholm. The measurements were performed during the summer 2005 and the data was used to test the software package for inversion methods, SAGA (Seismo Acoustic inversion using Genetic Algorithms, by Peter Gerstoft UCSD, US). Refractivity profiles retrieved by SAGA were compared with the refractivity profiles calculated from measured parameters, during parts of the experiment, from rocket sounding, radio sounding, local meteorological measurements using bulk model calculations, and also obtained by the Swedish operational weather forecast model HIRLAM. Surface based duct height are predicted in relative many situations even though the number of frequencies or antennas height has to be increased to diminish the ambiguous of the refractive index profile.

  17. Retrieval of Sea-Bed Parameters by the Method of Matching Acoustic Fields on the Basis of Vertical Angular Spectra

    NASA Astrophysics Data System (ADS)

    Kerzhakov, B. V.; Kulinich, V. V.

    2016-08-01

    We use the field matching method to solve the inverse problem of estimating the geoacoustic parameters of a stratified sea bed using the objective function based on the norm of difference between the experimental and simulated vertical angular spectra of the acoustic field and combination of the rapid-annealing method with direct search methods for localization of the global minimum of the objective function. To reduce the influence of the ravine effects of the objective function, we use regularization on the basis of mutual correlations of the experimental and simulated vertical angular spectra of the acoustic field. The numerical experiment has been performed to retrieve the parameters of the model waveguide, e.g., the thickness of the water layer and the layer of sediments, the velocity and attenuation coefficients of longitudinal waves, and the density of the sediment layer and the subjacent half-space in the presence of noise interference of different intensity levels.

  18. Immunonucleochemistry: a new method for in situ detection of antigens in the nucleus of cells in culture.

    PubMed

    Moon, Il Soo; Lee, Hyunsook; Park, Sung Dong; Seog, Dae-Hyun

    2010-04-01

    The advancement of immunocytochemistry (ICC) allows one to observe detailed spatial distribution of cellular antigens, but, with some limitations. Using conventional ICC, it is difficult to distinguish the nuclear localization from cytoplasm, as two large subcellular compartments overlap on the z-axis. In this study, we have investigated whether in situ immunostaining of 'naked' nuclei could provide an unambiguous method for detection of nuclear antigens. We have designed a protocol that efficiently lyses plasmalemma, while keeping the nuclear envelope intact. The optimal condition for lysing the plasmalemma was 0.5% Nonidet P-40 for 5 min in both neuronal and non-neuronal cultured cells. Using this protocol, we could unambiguously isolate nuclear from cytoplasmic ICC signals. Since the present protocol has been designed for immunostaining of 'naked' nuclei from cultured or isolated cells, we have coined a new term to refer to this procedure as 'immunonucleochemistry' ('INC' for abbreviation).

  19. Human factors analysis and design methods for nuclear waste retrieval systems. Human factors design methodology and integration plan

    SciTech Connect

    Casey, S.M.

    1980-06-01

    The purpose of this document is to provide an overview of the recommended activities and methods to be employed by a team of human factors engineers during the development of a nuclear waste retrieval system. This system, as it is presently conceptualized, is intended to be used for the removal of storage canisters (each canister containing a spent fuel rod assembly) located in an underground salt bed depository. This document, and the others in this series, have been developed for the purpose of implementing human factors engineering principles during the design and construction of the retrieval system facilities and equipment. The methodology presented has been structured around a basic systems development effort involving preliminary development, equipment development, personnel subsystem development, and operational test and evaluation. Within each of these phases, the recommended activities of the human engineering team have been stated, along with descriptions of the human factors engineering design techniques applicable to the specific design issues. Explicit examples of how the techniques might be used in the analysis of human tasks and equipment required in the removal of spent fuel canisters have been provided. Only those techniques having possible relevance to the design of the waste retrieval system have been reviewed. This document is intended to provide the framework for integrating human engineering with the rest of the system development effort. The activities and methodologies reviewed in this document have been discussed in the general order in which they will occur, although the time frame (the total duration of the development program in years and months) in which they should be performed has not been discussed.

  20. Phase retrieval and time-frequency methods in the measurement of ultrashort laser pulses

    SciTech Connect

    DeLong, K.W.; Fittinghoff, D.N.; Ladera, C.L.; Trebino, R.

    1995-02-01

    Recently several techniques have become available to measure the time- (or frequency-) dependent intensity and phase of ultrashort laser pulses. One of these, Frequency-Resolved Optical Gating (FROG), is rigorous and has achieved single-laser-shot operation. FROG combines the concepts of time-frequency analysis in the form of spectrogram generation (in order to create a two-dimensional problem), and uses a phase-retrieval-based algorithm to invert the experimental data to yield the intensity and phase of the laboratory laser pulse. In FROG it is easy to generate a spectrogram of the unknown signal, and inversion of the spectrogram to recover the signal is the main goal. Because the temporal width of a femtosecond laser pulse is much shorter than anything achievable by electronics, FROG uses the pulse to measure itself. In FROG, the laser pulse is split into two replicas of itself by a partially reflecting beamsplitter, and the two replicas interact with each other in a medium with an instantaneous nonlinear-optical response. This interaction generates a signal field that is then frequency-resolved using a spectrometer. The spectrum of the signal field is measured for all relevant values of the temporal delay between the two pulses. Here, the authors employ FROG and FROG related techniques to measure the time-dependent intensity and phase of an ultrashort laser pulse.

  1. A Comparison Between Heliosat-2 and Artificial Neural Network Methods for Global Horizontal Irradiance Retrievals over Desert Environments

    NASA Astrophysics Data System (ADS)

    Ghedira, H.; Eissa, Y.

    2012-12-01

    Global horizontal irradiance (GHI) retrievals at the surface of any given location could be used for preliminary solar resource assessments. More accurately, the direct normal irradiance (DNI) and diffuse horizontal irradiance (DHI) are also required to estimate the global tilt irradiance, mainly used for fixed flat plate collectors. Two different satellite-based models for solar irradiance retrievals have been applied over the desert environment of the United Arab Emirates (UAE). Both models employ channels of the SEVIRI instrument, onboard the geostationary satellite Meteosat Second Generation, as their main inputs. The satellite images used in this study have a temporal resolution of 15-min and a spatial resolution of 3-km. The objective of this study is to compare between the GHI retrieved using the Heliosat-2 method and an artificial neural network (ANN) ensemble method over the UAE. The high-resolution visible channel of SEVIRI is used in the Heliosat-2 method to derive the cloud index. The cloud index is then used to compute the cloud transmission, while the cloud-free GHI is computed from the Linke turbidity factor. The product of the cloud transmission and the cloud-free GHI denotes the estimated GHI. A constant underestimation is observed in the estimated GHI over the dataset available in the UAE. Therefore, the cloud-free DHI equation in the model was recalibrated to fix the bias. After recalibration, results over the UAE show a root mean square error (RMSE) value of 10.1% and a mean bias error (MBE) of -0.5%. As for the ANN approach, six thermal channels of SEVIRI were used to estimate the DHI and the total optical depth of the atmosphere (δ). An ensemble approach is employed to obtain a better generalizability of the results, as opposed to using one single weak network. The DNI is then computed from the estimated δ using the Beer-Bouguer-Lambert law. The GHI is computed from the DNI and DHI estimates. The RMSE for the estimated GHI obtained over an

  2. A comparison of DNA collection and retrieval from two swab types (cotton and nylon flocked swab) when processed using three QIAGEN extraction methods.

    PubMed

    Brownlow, Robert J; Dagnall, Kathryn E; Ames, Carole E

    2012-05-01

    The Metropolitan Police Service currently uses cotton swabs to retrieve DNA for forensic profiling. Recently, a new nylon flocked swab type has become available from Copan (MicroRheologics, Brescia, Italy) that it is claimed, offers increased sample recovery and release yields. If true, the flocked swab may have important applications in DNA evidence retrieval. This study examines the DNA retrieval capability of cotton and nylon flocked swabs when extracted using three common extraction platforms (QIAcube, BioRobot EZ1 and manually processed QIAamp DNA investigator kit). Results indicate that both swab types are capable of recovering high percentages of DNA (>50%); however, the extraction platform selected was shown to have a significant effect upon DNA retrieval. Across all experiments, the cotton swab combined with the spin-column extractions was shown to be most effective, with the nylon swab and BioRobot EZ1 combination being the least effective. These findings illustrate the importance of extraction method selection.

  3. The limits of ultrahigh-resolution x-ray mapping: estimating uncertainties in thin-film and interface structures determined by phase retrieval methods

    SciTech Connect

    Zhou H.; Pindak R.; Clarke, R.; Steinberg, D.NM.; Yacoby, Y.

    2012-04-25

    Capturing subtle details at the sub-Angstrom level is key to understanding the structural basis of many intriguing interfacial phenomena in epitaxial thin films and nanostructures. X-ray phase retrieval methods are ideally suited to this task but the usual approaches for determination of uncertainties, based on refining a parametrized model, are not applicable in this case. Here we describe a method to estimate the uncertainties of the system electron density, obtained by phase retrieval, and of parameters of interest obtained from it. The method is based on the bootstrap approach and it can be generally applied to surface x-ray scattering data. Several examples are given which illustrate the method's utility in determining uncertainties arising from random and systematic errors. The approach also provides a quantitative measure of the validity of structural solutions obtained by phase retrieval methods.

  4. The limits of ultrahigh-resolution x-ray mapping: estimating uncertainties in thin-film and interface structures determined by phase retrieval methods

    NASA Astrophysics Data System (ADS)

    Zhou, Hua; Pindak, Ron; Clarke, Roy; Steinberg, David M.; Yacoby, Yizhak

    2012-05-01

    Capturing subtle details at the sub-Angstrom level is key to understanding the structural basis of many intriguing interfacial phenomena in epitaxial thin films and nanostructures. X-ray phase retrieval methods are ideally suited to this task but the usual approaches for determination of uncertainties, based on refining a parametrized model, are not applicable in this case. Here we describe a method to estimate the uncertainties of the system electron density, obtained by phase retrieval, and of parameters of interest obtained from it. The method is based on the bootstrap approach and it can be generally applied to surface x-ray scattering data. Several examples are given which illustrate the method's utility in determining uncertainties arising from random and systematic errors. The approach also provides a quantitative measure of the validity of structural solutions obtained by phase retrieval methods.

  5. Methods for Retrievals of CO2 Mixing Ratios from JPL Laser Absorption Spectrometer Flights During a Summer 2011 Campaign

    NASA Technical Reports Server (NTRS)

    Menzies, Robert T.; Spiers, Gary D.; Jacob, Joseph C.

    2013-01-01

    The JPL airborne Laser Absorption Spectrometer instrument has been flown several times in the 2007-2011 time frame for the purpose of measuring CO2 mixing ratios in the lower atmosphere. This instrument employs CW laser transmitters and coherent detection receivers in the 2.05- micro m spectral region. The Integrated Path Differential Absorption (IPDA) method is used to retrieve weighted CO2 column mixing ratios. We present key features of the evolving LAS signal processing and data analysis algorithms and the calibration/validation methodology. Results from 2011 flights in various U.S. locations include observed mid-day CO2 drawdown in the Midwest and high spatial resolution plume detection during a leg downwind of the Four Corners power plant in New Mexico.

  6. An automatic Planetary Boundary Layer height retrieval method with compact EZ backscattering Lidar in the frame of ICOS campaign

    NASA Astrophysics Data System (ADS)

    Loaec, S.; Lolli, S.; Sauvage, L.; Boquet, M.; Xueref-Remy, I.

    2009-12-01

    Bigger strongly urbanized cities in the world are often exposed to atmospheric pollution events. To understand the chemical and physical processes that are taking place in these areas it is necessary to describe correctly the Planetary Boundary Layer (PBL) dynamics and the PBL height evolution. For these proposals, a compact and rugged eye safe UV Lidar, the EZLIDAR™, was developed together by CEA/LMD and LEOSPHERE (France) to study and investigate structural and optical properties of clouds and aerosols and PBL time evolution. EZLIDAR™ has been validated by different remote and in-situ instruments as MPL Type-4 Lidar manufactured by NASA at ARM/SGP site or the LNA (Lidar Nuage Aerosol) at the Laboratoire de Metereologie Dynamique LMD (France) and during several intercomparison campaigns. EZLIDAR™ algorithm retrieves automatically the PBL height in real-time. The method is based on the detection of the slope of the signal linked to a sharp change in concentration of the aerosols. Once detected, the different layers are filtered on a 15mn sample and classified between nocturnal, convective or residual layer, depending on the time and date. This method has been validated against those retrieved by the algorithm STRAT from data acquired at IPSL, France, showing 95% of correlation. In this paper are presented the results of the intercomparison campaign that took place in Orleans, France and Mace Head, Ireland in the framework of ICOS (Integrated Carbon Observation System) project, where the EZ Lidar™ worked under all weather conditions, clear sky, fog, low clouds, during the whole month of October 2008. Moreover, thanks to its 3D scanning capability, the EZLIDAR was able to provide the variability of the PBL height around the site, enabling the scientists to estimate the flux intensities that play a key role in the radiative transfer budget and in the atmospheric pollutants dispersion.

  7. Effects of histoplasmin M antigen chemical and enzymatic deglycosylation on cross-reactivity in the enzyme-linked immunoelectrotransfer blot method.

    PubMed Central

    Zancopé-Oliveira, R M; Bragg, S L; Reiss, E; Wanke, B; Peralta, J M

    1994-01-01

    The enzyme-linked immunoelectrotransfer blot (EITB) method was evaluated as a suitable method for detecting antibodies against M antigen of Histoplasma capsulatum by use of both glycosylated and deglycosylated M protein of histoplasmin (HMIN). Sera from patients with histoplasmosis, paracoccidioidomycosis, blastomycosis, coccidioidomycosis, and aspergillosis were tested by the EITB with glycosylated M protein of HMIN. This assay demonstrated 100% sensitivity with histoplasmosis serum samples, all of which reacted with the 94-kDa glycoprotein (M antigen). Although the EITB is highly sensitive, it is not specific for histoplasmosis when glycosylated M protein is used as an antigen. A total of 81% of paracoccidioidomycosis, 25% of blastomycosis, 33% of coccidioidomycosis, 73% of aspergillosis, and 16% of tuberculosis serum samples cross-reacted with M protein of HMIN and yielded patterns indistinguishable from those obtained with histoplasmosis serum samples. The EITB reactions with both untreated M antigen and M antigen altered by periodate oxidation or by deglycosylation with endoglycosidases were compared. Cross-reactions with heterologous sera in the EITB could be attributed to periodate-sensitive carbohydrate epitopes, as reflected by the increase in the test specificity from 46.1 to 91.2% after periodate treatment of M protein. The EITB for the detection of antibodies to M antigen is a potential diagnostic test for histoplasmosis, provided that periodate-treated M protein is used as an antigen. Images PMID:8556474

  8. Flow cytometry-based methods for assessing soluble scFv activities and detecting pathogen antigens in solution

    SciTech Connect

    Gray, Sean; Weigel, Kris M.; Miller, Keith D.; Ndung'u, Joseph; Buscher, Philippe; Tran, Thao N.; Baird, Cheryl L.; Cangelosi, Gerard A.

    2010-04-01

    Novel methods are reported for evaluating and utilizing single chain fragment variable (scFv) antibodies derived from yeast-display libraries. Yeast-display was used to select scFv specific to invariant surface glycoproteins (ISG) of Trypanosoma brucei. A limiting step in the isolation of scFv from nonimmune libraries is the conversion of highly active yeast-displayed scFv into soluble antibodies that can be used in standard immunoassays. Challenges include limited solubility or activity following secretion and purification of scFv. For this reason, few scFv derived from yeast-display platforms have moved into development and implementation as diagnostic reagents. To address this problem, assays were developed that employ both yeastdisplayed and secreted scFv as analytical reagents. The first is a competitive inhibition flow cytometry (CIFC) assay that detects secreted scFv by virtue of its ability to competitively inhibit the binding of biotinylated antigen to yeast-displayed scFv. The second is an epitope binning assay that uses secreted scFv toidentify additional yeast-displayed scFv that bind nonoverlapping or noncompeting epitopes on an antigen. The epitope binning assay was used not only to identify sandwich assay pairs with yeast-displayed scFv, but also to identify active soluble scFv present in low concentration in a crude expression extract. Finally, a CIFC assay was developed that bypasses entirely the need for soluble scFv expression, by using yeast displayed scFv to detect unlabeled antigen in samples. These methods will facilitate the continued development and practical implementation of scFv derived from yeast-display libraries.

  9. Methods for semi-automated indexing for high precision information retrieval

    NASA Technical Reports Server (NTRS)

    Berrios, Daniel C.; Cucina, Russell J.; Fagan, Lawrence M.

    2002-01-01

    OBJECTIVE: To evaluate a new system, ISAID (Internet-based Semi-automated Indexing of Documents), and to generate textbook indexes that are more detailed and more useful to readers. DESIGN: Pilot evaluation: simple, nonrandomized trial comparing ISAID with manual indexing methods. Methods evaluation: randomized, cross-over trial comparing three versions of ISAID and usability survey. PARTICIPANTS: Pilot evaluation: two physicians. Methods evaluation: twelve physicians, each of whom used three different versions of the system for a total of 36 indexing sessions. MEASUREMENTS: Total index term tuples generated per document per minute (TPM), with and without adjustment for concordance with other subjects; inter-indexer consistency; ratings of the usability of the ISAID indexing system. RESULTS: Compared with manual methods, ISAID decreased indexing times greatly. Using three versions of ISAID, inter-indexer consistency ranged from 15% to 65% with a mean of 41%, 31%, and 40% for each of three documents. Subjects using the full version of ISAID were faster (average TPM: 5.6) and had higher rates of concordant index generation. There were substantial learning effects, despite our use of a training/run-in phase. Subjects using the full version of ISAID were much faster by the third indexing session (average TPM: 9.1). There was a statistically significant increase in three-subject concordant indexing rate using the full version of ISAID during the second indexing session (p < 0.05). SUMMARY: Users of the ISAID indexing system create complex, precise, and accurate indexing for full-text documents much faster than users of manual methods. Furthermore, the natural language processing methods that ISAID uses to suggest indexes contributes substantially to increased indexing speed and accuracy.

  10. Methods for Semi-automated Indexing for High Precision Information Retrieval

    PubMed Central

    Berrios, Daniel C.; Cucina, Russell J.; Fagan, Lawrence M.

    2002-01-01

    Objective. To evaluate a new system, ISAID (Internet-based Semi-automated Indexing of Documents), and to generate textbook indexes that are more detailed and more useful to readers. Design. Pilot evaluation: simple, nonrandomized trial comparing ISAID with manual indexing methods. Methods evaluation: randomized, cross-over trial comparing three versions of ISAID and usability survey. Participants. Pilot evaluation: two physicians. Methods evaluation: twelve physicians, each of whom used three different versions of the system for a total of 36 indexing sessions. Measurements. Total index term tuples generated per document per minute (TPM), with and without adjustment for concordance with other subjects; inter-indexer consistency; ratings of the usability of the ISAID indexing system. Results. Compared with manual methods, ISAID decreased indexing times greatly. Using three versions of ISAID, inter-indexer consistency ranged from 15% to 65% with a mean of 41%, 31%, and 40% for each of three documents. Subjects using the full version of ISAID were faster (average TPM: 5.6) and had higher rates of concordant index generation. There were substantial learning effects, despite our use of a training/run-in phase. Subjects using the full version of ISAID were much faster by the third indexing session (average TPM: 9.1). There was a statistically significant increase in three-subject concordant indexing rate using the full version of ISAID during the second indexing session (p < 0.05). Summary. Users of the ISAID indexing system create complex, precise, and accurate indexing for full-text documents much faster than users of manual methods. Furthermore, the natural language processing methods that ISAID uses to suggest indexes contributes substantially to increased indexing speed and accuracy. PMID:12386114

  11. A new method to retrieve spectral absorption coefficient of highly-scattering and weakly-absorbing materials

    NASA Astrophysics Data System (ADS)

    Dombrovsky, Leonid A.

    2016-03-01

    A significant uncertainty in the absorption coefficient of highly scattering dispersed materials is typical in the spectral ranges of very weak absorption. The traditional way to identify the main absorption and scattering characteristics of semi-transparent materials is based on spectral measurements of normal-hemispherical reflectance and transmittance for the material sample. Unfortunately this way cannot be used in the case of in vivo measurements of optical properties of biological tissues. A method suggested in the present paper is based on thermal response to the periodic radiative heating of the open surface of a semi-transparent material. It is shown that the period of a variation of the surface temperature is sensitive to the value of an average absorption coefficient in the surface layer. As a result, the monochromatic external irradiation combined with the surface temperature measurements can be used to retrieve the spectral values of absorption coefficient. Possible application of this method to porous semi-transparent ceramics is considered. An example problem is also solved to illustrate the applicability of this method to human skin. The approach suggested enables one to estimate an average absorption coefficient of human skin of a patient just before the thermal processing.

  12. A Method to Retrieve the Multi-Receiver Moho Reflection Response from SH-Wave Scattering Coda in the Radiative Transfer Regime

    NASA Astrophysics Data System (ADS)

    Hartstra, I.; Wapenaar, C. P. A.

    2015-12-01

    We discuss a method to retrieve the multi-receiver Moho reflection response by interferometry from SH-wave coda in the 0.5-3 Hz frequency range. An image derived from a reflection response with a well defined virtual source would provide deterministic impedance contrasts, which can complement transmission tomography. For an accurate retrieval, cross-correlation interferometry requires the coda wave field to sample the imaging target and isotropically illuminate the receiver array. When these illumination requirements are not or only partially met, the stationary phase cannot be fully captured and artifacts will contaminate the retrieved reflection response. Here we conduct numerical scalar 2D finite difference simulations to investigate the challenging situation in which only shallow crustal earthquake sources illuminate the Moho and the response is recorded by a 2D linear array. We quantify to what extent the prevalence of scatterers in the crust can improve the illumination conditions and thus the retrieval of the Moho reflection. The accuracy of the retrieved reflection is evaluated for two physically different scattering regimes: the Rayleigh and Mie regime. We only use the earlier part of the scattering coda, because we have found that the later diffusive part does not significantly improve the retrieval. The density of the spherical scatterers is varied in order to change the scattering mean free path. This characteristic length scale is calculated for each model with the 2D radiative transfer equation, which is the governing equation in the earlier part of the scattering coda. The experiment is repeated for models of different geological settings derived from existing S-wave tomographies, which vary in Moho depth and reflectivity. The scattering mean free path can be approximated for real data if intrinsic attenuation is known, because the wavenumber-dependent scattering attenuation of the coherent wave amplitude is dependent on the scattering mean free path

  13. Method for Face-Emotion Retrieval Using A Cartoon Emotional Expression Approach

    NASA Astrophysics Data System (ADS)

    Kostov, Vlaho; Yanagisawa, Hideyoshi; Johansson, Martin; Fukuda, Shuichi

    A simple method for extracting emotion from a human face, as a form of non-verbal communication, was developed to cope with and optimize mobile communication in a globalized and diversified society. A cartoon face based model was developed and used to evaluate emotional content of real faces. After a pilot survey, basic rules were defined and student subjects were asked to express emotion using the cartoon face. Their face samples were then analyzed using principal component analysis and the Mahalanobis distance method. Feature parameters considered as having relations with emotions were extracted and new cartoon faces (based on these parameters) were generated. The subjects evaluated emotion of these cartoon faces again and we confirmed these parameters were suitable. To confirm how these parameters could be applied to real faces, we asked subjects to express the same emotions which were then captured electronically. Simple image processing techniques were also developed to extract these features from real faces and we then compared them with the cartoon face parameters. It is demonstrated via the cartoon face that we are able to express the emotions from very small amounts of information. As a result, real and cartoon faces correspond to each other. It is also shown that emotion could be extracted from still and dynamic real face images using these cartoon-based features.

  14. Methods for Heel Retrieval for Tanks C-101, C-102, and C-111 at the Hanford Site - 13064

    SciTech Connect

    Sams, T.L.; Kirch, N.W.; Reynolds, J.H.

    2013-07-01

    The purpose of this paper is to evaluate the prospects of using bulk waste characteristics to determine the most appropriate heel retrieval technology. If the properties of hard to remove heels can be determined before bulk retrieval, then a heel retrieval technology can be selected before bulk retrieval is complete. This would save substantially on sampling costs and would allow the deployment of the heel retrieval technology immediately after bulk retrieval. The latter would also accelerate the heel removal schedule. A number of C-farm retrievals have been fully or partially completed at the time of this writing. Thus, there is already substantial information on the success of different technologies and the composition of the heels. There is also substantial information on the waste types in each tank based on historical records. Therefore, this study will correlate the performance of technologies used so far and compare them to the known waste types in the tanks. This will be used to estimate the performance of future C Farm heel retrievals. An initial decision tree is developed and employed on tanks C-101, C-102, and C 111. An assumption of this study is that no additional characterization information would be available, before or after retrieval. Note that collecting additional information would substantially increase the probability of success. Deploying some in-situ testing technologies, such as a water lance or an in-situ Raman probe, might substantially increase the probability of successfully selecting the process conditions without having to take samples from the tanks for laboratory analysis. (authors)

  15. Methods for heel retrieval for tanks C-101, C-102, and C-111 at the Hanford Site

    SciTech Connect

    Sams, Terry L.; Kirch, N. W.; Reynolds, Jacob G.

    2013-01-11

    The purpose of this paper is to evaluate the prospects of using bulk waste characteristics to determine the most appropriate heel retrieval technology. If the properties of hard to remove heels can be determined before bulk retrieval, then a heel retrieval technology can be selected before bulk retrieval is complete. This would save substantially on sampling costs and would allow the deployment of the heel retrieval technology immediately after bulk retrieval. The latter would also accelerate the heel removal schedule. A number of C-farm retrievals have been fully or partially completed at the time of this writing. Thus, there is already substantial information on the success of different technologies and the composition of the heels. There is also substantial information on the waste types in each tank based on historical records. Therefore, this study will correlate the performance of technologies used so far and compare them to the known waste types in the tanks. This will be used to estimate the performance of future C Farm heel retrievals. An initial decision tree is developed and employed on tanks C-101, C-102, and C 111. An assumption of this study is that no additional characterization information would be available, before or after retrieval. Note that collecting additional information would substantially increase the probability of success. Deploying some in-situ testing technologies, such as a water lance or an in-situ Raman probe, might substantially increase the probability of successfully selecting the process conditions without having to take samples from the tanks for laboratory analysis.

  16. An osmium-free method of epon embedment that preserves both ultrastructure and antigenicity for post-embedding immunocytochemistry.

    PubMed

    Phend, K D; Rustioni, A; Weinberg, R J

    1995-03-01

    Immunocytochemistry for amino acids with post-embedding gold is compatible with glutaraldehyde fixation, osmication, and embedding in epoxy-based plastics, but immunogold detection of larger molecules in the central nervous system commonly requires special procedures, e.g. minimizing exposure to glutaraldehyde, eliminating osmium, cryosectioning, and/or embedding in acrylic plastics. These make samples more difficult to prepare and view and may compromise structural preservation. We report a new technique, fixing with high levels of glutaraldehyde, replacing osmium with tannic acid followed by other heavy metals and p-phenylenediamine, and embedding in Epon. This method optimizes antigenicity while retaining the structural preservation and convenient handling of standard embedding techniques. Compared to standard Epon embedment, labeling for neuropeptides in brain and spinal cord is improved. Moreover, the present method yields excellent labeling of glutamate receptors (difficult to identify with traditional post-embedding techniques) and enables simultaneous visualization of associated neurotransmitters.

  17. Methods of atomic oxygen and ozone retrieval from observations of the O2 dayglow emissions in the MLT region

    NASA Astrophysics Data System (ADS)

    Yankovsky, Valentine; Martyshenko, Kseniia; Manuilova, Rada

    2015-04-01

    The problem of creating the new methods of remote sensing of altitude profile of the [O(3P)] and [O3] in the daytime is actual for the mesosphere and lower thermosphere range. Currently there is no reliable method for remote sensing of altitude profile of the [O(3P)], but atomic oxygen is a key component in the mechanism of the atmosphere cooling by quenching of vibrationally excited CO2 molecules and also one of basic quencher of excited components in MLT region. The airglow emission in 1.27 µm IR Atm(0 - 0) band from [O2(a1Δg, v=0)] has been used as a proxy for [O3] in MLT for over a decade. However, lifetime of O2(a1Δg, v=0) is more than 1 hour, therefore this method is not suitable for detecting of relatively rapid [O3] variations which occur due to the variability of the solar spectrum in the UV range (120 - 320 nm) and other space factors. The aim of this study is revealing of proxies for retrievals of [O(3P)] and [O3]. In the framework of developed model of electronic vibrational kinetics of excited products of O3 and O2 photolysis in MLT of the Earth (model YM-2011) [1] we consider the photolysis of O2 in the Schumann-Runge continuum and Lyaman-A H atom and of O3 in Hartley band and for excited products of photolysis ( O2(a1Δg, v=0 - 5), O2(b1Σ+g, v=0, 1, 2) and excited oxygen atom O(1D)) we took into account more than 60 aeronomical reactions of photoexcitation and deexcitation by energy transfer between the excited levels and of quenching of the levels in collisions with O(3P) O2, N2, O(3P), O3, CO2. We tested 5 excited components, namely, O2(b1Σ+g, v=0, 1, 2), O2(a1Δg, v=0 - 5) and O(1D) as the O(3P) and O3 proxies. The total system of kinetic equations for 10 components has been solved and altitude profiles of concentrations of O(1D), O2(b1Σ+g, v=0, 1, 2), and O2(a1Δg, v=0 - 5) have been calculated. To compare characteristics of assumed proxies we used sensitivity analysis of the proxy concentrations altitude profiles to variations of [O3] and

  18. Fast method of retrieving the asymmetry factor and scattering albedo from the maximum time-resolved reflectance of participating media.

    PubMed

    Qi, Hong; Ren, Ya-Tao; Chen, Qin; Ruan, Li-Ming

    2015-06-01

    This research presents a parametric study of the time-resolved hemispherical reflectance of a semi-infinite plane-parallel slab of homogeneous, nonemitting, absorbing, and anisotropic scattering medium exposed to a collimated Gaussian pulse. The one-dimensional transient radiative transfer equation was solved by using the finite volume method. The internal reflection at the medium-air interface caused by the mismatch of the refractive indices was considered. In particular, this work focused on the maximum diffuse hemispherical reflectance. Three different optical regions were identified according to the dimensionless pulsewidth βctp. The correlation between the normalized maximum hemispherical reflectance and βctp was conformed to the Boltzmann function. The coefficients in the correlating functions of the match and mismatch refractive index cases were fitted as polynomial fitting functions of the single scattering albedo ω and Henyey-Greenstein asymmetric factor g. Thus, ω and g can be simultaneously reconstructed by the semi-empirical correlations without solving the forward model. In conclusion, the proposed method can potentially retrieve the asymmetry factor and single scattering albedo of participating media accurately and efficiently.

  19. Gene-Disease Interaction Retrieval from Multiple Sources: A Network Based Method

    PubMed Central

    Huang, Lan; Wang, Yan

    2016-01-01

    The number of gene-related databases has been growing largely along with the research on genes of bioinformatics. Those databases are filled with various gene functions, pathways, interactions, and so forth, while much biomedical knowledge about human diseases is stored as text in all kinds of literatures. Researchers have developed many methods to extract structured biomedical knowledge. Some study and improve text mining algorithms to achieve efficiency in order to cover as many data sources as possible, while some build open source database to accept individual submissions in order to achieve accuracy. This paper combines both efforts and biomedical ontologies to build an interaction network of multiple biomedical ontologies, which guarantees its robustness as well as its wide coverage of biomedical publications. Upon the network, we accomplish an algorithm which discovers paths between concept pairs and shows potential relations. PMID:27478829

  20. Localization of hepatitis B surface antigen in conventional paraffin sections of the liver. Comparison of immunofluorescence, immunoperoxidase, and orcein staining methods with regard to their specificity and reliability as antigen marker.

    PubMed Central

    Nayak, N. C.; Sachdeva, R.

    1975-01-01

    Hepatitis B antigen (HBAg) has been demonstrated in conventional formalin-fixed paraffin-embedded liver tissue by peroxidase and fluorescent immunostaining as well as by orcein. Complete locational and morphologic identity is seen between material stained by specific immunologic methods and by orcein. The antigen is restricted to the cytoplasm and is generally observed in the hepatocyte; it is present in three morphologic forms. Certain morphologic forms can even be identified in hematoxylin and eosin-stained tissue. Results of immunostaining procedures indicate that the antigen demonstrated in this study consists entirely of surface coat of hepatitis B virus (HBsAg). This seems to be the only component revealed by orcein staining. The latter is considered to be a good marker of the surface antigen and to have certain advantages over immunostaining. It is suggested that suitability of conventional paraffin sections for the detection of HBAg has wide and important implications. Images Figures 1-5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 PMID:55076

  1. Characterization of anthropogenic methane plumes with the Hyperspectral Thermal Emission Spectrometer (HyTES): a retrieval method and error analysis

    NASA Astrophysics Data System (ADS)

    Kuai, Le; Worden, John R.; Li, King-Fai; Hulley, Glynn C.; Hopkins, Francesca M.; Miller, Charles E.; Hook, Simon J.; Duren, Riley M.; Aubrey, Andrew D.

    2016-07-01

    We introduce a retrieval algorithm to estimate lower tropospheric methane (CH4) concentrations from the surface to 1 km with uncertainty estimates using Hyperspectral Thermal Emission Spectrometer (HyTES) airborne radiance measurements. After resampling, retrievals have a spatial resolution of 6 × 6 m2. The total error from a single retrieval is approximately 20 %, with the uncertainties determined primarily by noise and spectral interferences from air temperature, surface emissivity, and atmospheric water vapor. We demonstrate retrievals for a HyTES flight line over storage tanks near Kern River Oil Field (KROF), Kern County, California, and find an extended plume structure in the set of observations with elevated methane concentrations (3.0 ± 0.6 to 6.0 ± 1.2 ppm), well above mean concentrations (1.8 ± 0.4 ppm) observed for this scene. With typically a 20 % estimated uncertainty, plume enhancements with more than 1 ppm are distinguishable from the background values with its uncertainty. HyTES retrievals are consistent with simultaneous airborne and ground-based in situ CH4 mole fraction measurements within the reported accuracy of approximately 0.2 ppm (or ˜ 8 %), due to retrieval interferences related to air temperature, emissivity, and H2O.

  2. A new method for fusion, denoising and enhancement of x-ray images retrieved from Talbot-Lau grating interferometry

    NASA Astrophysics Data System (ADS)

    Scholkmann, Felix; Revol, Vincent; Kaufmann, Rolf; Baronowski, Heidrun; Kottler, Christian

    2014-03-01

    This paper introduces a new image denoising, fusion and enhancement framework for combining and optimal visualization of x-ray attenuation contrast (AC), differential phase contrast (DPC) and dark-field contrast (DFC) images retrieved from x-ray Talbot-Lau grating interferometry. The new image fusion framework comprises three steps: (i) denoising each input image (AC, DPC and DFC) through adaptive Wiener filtering, (ii) performing a two-step image fusion process based on the shift-invariant wavelet transform, i.e. first fusing the AC with the DPC image and then fusing the resulting image with the DFC image, and finally (iii) enhancing the fused image to obtain a final image using adaptive histogram equalization, adaptive sharpening and contrast optimization. Application examples are presented for two biological objects (a human tooth and a cherry) and the proposed method is compared to two recently published AC/DPC/DFC image processing techniques. In conclusion, the new framework for the processing of AC, DPC and DFC allows the most relevant features of all three images to be combined in one image while reducing the noise and enhancing adaptively the relevant image features. The newly developed framework may be used in technical and medical applications.

  3. A new method for fusion, denoising and enhancement of x-ray images retrieved from Talbot-Lau grating interferometry.

    PubMed

    Scholkmann, Felix; Revol, Vincent; Kaufmann, Rolf; Baronowski, Heidrun; Kottler, Christian

    2014-03-21

    This paper introduces a new image denoising, fusion and enhancement framework for combining and optimal visualization of x-ray attenuation contrast (AC), differential phase contrast (DPC) and dark-field contrast (DFC) images retrieved from x-ray Talbot-Lau grating interferometry. The new image fusion framework comprises three steps: (i) denoising each input image (AC, DPC and DFC) through adaptive Wiener filtering, (ii) performing a two-step image fusion process based on the shift-invariant wavelet transform, i.e. first fusing the AC with the DPC image and then fusing the resulting image with the DFC image, and finally (iii) enhancing the fused image to obtain a final image using adaptive histogram equalization, adaptive sharpening and contrast optimization. Application examples are presented for two biological objects (a human tooth and a cherry) and the proposed method is compared to two recently published AC/DPC/DFC image processing techniques. In conclusion, the new framework for the processing of AC, DPC and DFC allows the most relevant features of all three images to be combined in one image while reducing the noise and enhancing adaptively the relevant image features. The newly developed framework may be used in technical and medical applications.

  4. Comparison between the ESFT method and LBL method of CO2 retrieval for high-resolution satellite

    NASA Astrophysics Data System (ADS)

    Li, Yanfen; Zhang, Chunmin; Wang, Dingyi; Chen, Jie; Liu, Dongdong; Rong, Piao

    2015-02-01

    The spectra of O2 A-band (0.76 μm) and CO2 near-infrared emissions (1.6 μm) for Medium-resolution Satellite (SCIAMACHY) are simulated by the SCIATRAN model (V3.1.29), and compared with the ESFT and LBL method, as the inversion accuracy and time consuming. The time consuming of LBL was more than ESFT with the relative error less than 1%, especially for the CO2 band. But for the CO2 (2.0 um) of High-resolution Satellite, the opposite result was found. That is to say, the LBL method was more suitable for High-resolution Satellite. Different wavelength intervals and integral wavelength steps are applied to the LBL to select the most appropriate combination for High-resolution SatelliteO2 A-band (0.76 μm) and CO2 near-infrared band (1.58 μm).

  5. Retrievable Inferior Vena Cava Filters: Factors that Affect Retrieval Success

    SciTech Connect

    Geisbuesch, Philipp Benenati, James F.; Pena, Constantino S.; Couvillon, Joseph; Powell, Alex; Gandhi, Ripal; Samuels, Shaun; Uthoff, Heiko

    2012-10-15

    Purpose: To report and analyze the indications, procedural success, and complications of retrievable inferior vena cava filters (rIVCF) placement and to identify parameters that influence retrieval attempt and failure. Methods: Between January 2005 and December 2010, a total of 200 patients (80 men, median age 67 years, range 11-95 years) received a rIVCF with the clinical possibility that it could be removed. All patients with rIVCF were prospectively entered into a database and followed until retrieval or a decision not to retrieve the filter was made. A retrospective analysis of this database was performed. Results: Sixty-one percent of patients had an accepted indication for filter placement; 39% of patients had a relative indication. There was a tendency toward a higher retrieval rate in patients with relative indications (40% vs. 55%, P = 0.076). Filter placement was technically successful in all patients, with no procedure-related mortality. The retrieval rate was 53%. Patient age of >80 years (odds ratio [OR] 0.056, P > 0.0001) and presence of malignancy (OR 0.303, P = 0.003) was associated with a significantly reduced probability for attempted retrieval. Retrieval failure occurred in 7% (6 of 91) of all retrieval attempts. A time interval of > 90 days between implantation and attempted retrieval was associated with retrieval failure (OR 19.8, P = 0.009). Conclusions: Patient age >80 years and a history of malignancy are predictors of a reduced probability for retrieval attempt. The rate of retrieval failure is low and seems to be associated with a time interval of >90 days between filter placement and retrieval.

  6. A Solar Reflectance Method for Retrieving Cloud Optical Thickness and Droplet Size Over Snow and Ice Surfaces

    NASA Technical Reports Server (NTRS)

    Platnick, S.; Li, J. Y.; King, M. D.; Gerber, H.; Hobbs, P. V.

    1999-01-01

    Cloud optical thickness and effective radius retrievals from solar reflectance measurements are traditionally implemented using a combination of spectral channels that are absorbing and non-absorbing for water particles. Reflectances in non-absorbing channels (e.g., 0.67, 0.86, 1.2 micron spectral window bands) are largely dependent on cloud optical thickness, while longer wavelength absorbing channels (1.6, 2. 1, and 3.7 micron window bands) provide cloud particle size information. Cloud retrievals over ice and snow surfaces present serious difficulties. At the shorter wavelengths, ice is bright and highly variable, both characteristics acting to significantly increase cloud retrieval uncertainty. In contrast, reflectances at the longer wavelengths are relatively small and may be comparable to that of dark open water. A modification to the traditional cloud retrieval technique is devised. The new algorithm uses only a combination of absorbing spectral channels for which the snow/ice albedo is relatively small. Using this approach, retrievals have been made with the MODIS Airborne Simulator (MAS) imager flown aboard the NASA ER-2 from May - June 1998 during the Arctic FIRE-ACE field deployment. Data from several coordinated ER-2 and University of Washington CV-580 in situ aircraft observations of liquid water stratus clouds are examined. MAS retrievals of optical thickness, droplet effective radius, and liquid water path are shown to be in good agreement with the in situ measurements. The initial success of the technique has implications for future operational satellite cloud retrieval algorithms in polar and wintertime regions.

  7. Guenter Tulip Filter Retrieval Experience: Predictors of Successful Retrieval

    SciTech Connect

    Turba, Ulku Cenk Arslan, Bulent Meuse, Michael Sabri, Saher; Macik, Barbara Gail

    2010-08-15

    We report our experience with Guenter Tulip filter placement indications, retrievals, and procedural problems, with emphasis on alternative retrieval techniques. We have identified 92 consecutive patients in whom a Guenter Tulip filter was placed and filter removal attempted. We recorded patient demographic information, filter placement and retrieval indications, procedures, standard and nonstandard filter retrieval techniques, complications, and clinical outcomes. The mean time to retrieval for those who experienced filter strut penetration was statistically significant [F(1,90) = 8.55, p = 0.004]. Filter strut(s) IVC penetration and successful retrieval were found to be statistically significant (p = 0.043). The filter hook-IVC relationship correlated with successful retrieval. A modified guidewire loop technique was applied in 8 of 10 cases where the hook appeared to penetrate the IVC wall and could not be engaged with a loop snare catheter, providing additional technical success in 6 of 8 (75%). Therefore, the total filter retrieval success increased from 88 to 95%. In conclusion, the Guenter Tulip filter has high successful retrieval rates with low rates of complication. Additional maneuvers such as a guidewire loop method can be used to improve retrieval success rates when the filter hook is endothelialized.

  8. A method for retrieving vertical distribution of aerosol mass concentration in atmosphere from results of lidar sensing at Nd:YAG laser wavelengths

    NASA Astrophysics Data System (ADS)

    Lisenko, S. A.; Kugeiko, M. M.

    2011-03-01

    A method for retrieving the vertical profiles of atmospheric aerosol concentration from the results of lidar sensing at Ng:YAG laser wavelengths is developed based on the found multiple regressions between the optical location characteristics of aerosol at wavelengths of 0.355, 0.532, and 1.064 nm, as well as between the aerosol backscattering coefficient at these wavelengths and the concentration of aerosol particles. The method does not require solving ill-posed inverse problems and minimizes the use of a priori information. The reliability and generality of regressions obtained are confirmed by their good agreement with the AERO-NET data. The method efficiency is demonstrated by numerical experiments on retrieving profiles of back-scattering coefficients and concentration that corresponds to different optical models of aerosol.

  9. Demonstration of retrieval methods for Westinghouse Hanford Corporation by EPW Industrial Services Incorporated, trial number 2, April 30, 1996

    SciTech Connect

    1996-10-01

    Westinghouse Hanford Company has been pursuing strategies to break up and retrieve the radioactive waste material in single shell storage tanks at the Hanford Nuclear Reservation, by working with non-radioactive ``saltcake`` and sludge material that simulate the actual waste. Previous trials run by MPW for Westinghouse Hanford Company (report dated October 20, 1995) resulted in difficulty breaking up the simulant using three approaches, with pressures to I 0,000 psi and flow rates to 56 gpm. The saltcake sirnulant was extremely hard, with a tensile strength similar to concrete. Westinghouse Hanford proposed a second set of trials utilizing a range of four sirnulants to attempt to bracket the capability of the I 0,000 psi waterblast stream. This set of trials had one simulant similar to the soft sludge previously trialed, two similar to the hard saltcake but with a lower strength, and one with a coarse rock-salt in a saltcake matrix, which is quite low in strength. Under this proposal, MPW would attempt to dislodge the four different simulants us@@ng two methods, for a total of eight pans. Each pan would be 4-foot square x I- 1/2-foot deep. The approaches would be as follows: A. Rotary tooling approaching straight down, with two triangle nozzles, each at a nominal 12.5 gpm at I 0,000 psi, at a 45-degree angle. B. Straight in to the exposed edge of the material with an articulated ``water cannon`` -with a triangle nozzle rated at approximately 25 gpm.

  10. A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera.

    PubMed

    Whittemore, Kurt; Sykes, Kathryn

    2013-10-01

    The immune system responds to tumor cells. The challenge has been how to effectively use these responses to treat or protect against cancer. Toward the goal of developing a cancer vaccine, we are pursuing methodologies for the discovery and testing of useful antigens. We present an array-based approach for discovering these B cell antigens by directly screening for specific host-sera reactivity to lysates from tumor-derived cDNA expression libraries. Several cancer-specific antigens were identified, and these are currently being validated as potential candidates.

  11. A Framework for Quantifying the Impacts of Sub-Pixel Reflectance Variance and Covariance on Cloud Optical Thickness and Effective Radius Retrievals Based on the Bi-Spectral Method.

    NASA Technical Reports Server (NTRS)

    Zhang, Z; Werner, F.; Cho, H. -M.; Wind, Galina; Platnick, S.; Ackerman, A. S.; Di Girolamo, L.; Marshak, A.; Meyer, Kerry

    2017-01-01

    The so-called bi-spectral method retrieves cloud optical thickness (t) and cloud droplet effective radius (re) simultaneously from a pair of cloud reflectance observations, one in a visible or near infrared (VIS/NIR) band and the other in a shortwave-infrared (SWIR) band. A cloudy pixel is usually assumed to be horizontally homogeneous in the retrieval. Ignoring sub-pixel variations of cloud reflectances can lead to a significant bias in the retrieved t and re. In this study, we use the Taylor expansion of a two-variable function to understand and quantify the impacts of sub-pixel variances of VIS/NIR and SWIR cloud reflectances and their covariance on the t and re retrievals. This framework takes into account the fact that the retrievals are determined by both VIS/NIR and SWIR band observations in a mutually dependent way. In comparison with previous studies, it provides a more comprehensive understanding of how sub-pixel cloud reflectance variations impact the t and re retrievals based on the bi-spectral method. In particular, our framework provides a mathematical explanation of how the sub-pixel variation in VIS/NIR band influences the re retrieval and why it can sometimes outweigh the influence of variations in the SWIR band and dominate the error in re retrievals, leading to a potential contribution of positive bias to the re retrieval.

  12. A method to identify protein antigens of Dermanyssus gallinae for the protection of birds from poultry mites.

    PubMed

    Makert, Gustavo R; Vorbrüggen, Susanne; Krautwald-Junghanns, Maria-Elisabeth; Voss, Matthias; Sohn, Kai; Buschmann, Tilo; Ulbert, Sebastian

    2016-07-01

    The poultry red mite (PRM) Dermanyssus gallinae causes high economic losses and is among the most important parasites in poultry farming worldwide. Different chemical, physical, and biological strategies try to control the expansion of PRM. However, effective solutions to this problem still have to be found. Here, we present a method for the development of an immunological control strategy, based on the identification of mite protein antigens which elicit antibodies with anti-mite activity in the immunized chicken. Hens were immunized with different PRM protein extracts formulated with two different adjuvants, and IgY-antibodies were isolated from the eggs. A PRM in vitro feeding assay which used chicken blood spiked with these IgY-preparations was used to detect antibodies which caused PRM mortality. In vitro feeding of mites with IgY isolated from hens immunized with PRM extract formulated with one of the adjuvants showed a statistically significant increase in the mortality as compared to control mites. After the separation of total PRM extracts in two-dimensional gels, several protein spots were recognized by such IgY preparations. Ten protein spots were subjected to mass spectrometry (MS/MS) for the identification of the corresponding proteins. Complete protein sequences were deduced from genomic and transcriptomic assemblies derived from high throughput sequencing of total PRM DNA and RNA. The results may contribute to the development of an immunological control strategy of D. gallinae.

  13. Adaptive MGS Phase Retrieval

    NASA Technical Reports Server (NTRS)

    Basinger, Scott A.; Bikkannavar, Siddarayappa; Cohen, David; Green, Joseph J.; Lou, John; Ohara, Catherine; Redding, David; Shi, Fang

    2008-01-01

    Adaptive MGS Phase Retrieval software uses the Modified Gerchberg-Saxton (MGS) algorithm, an image-based sensing method that can turn any focal plane science instrument into a wavefront sensor, avoiding the need to use external metrology equipment. Knowledge of the wavefront enables intelligent control of active optical systems.

  14. Automatic Classification in Information Retrieval.

    ERIC Educational Resources Information Center

    van Rijsbergen, C. J.

    1978-01-01

    Addresses the application of automatic classification methods to the problems associated with computerized document retrieval. Different kinds of classifications are described, and both document and term clustering methods are discussed. References and notes are provided. (Author/JD)

  15. Generalized amplitude-phase retrieval algorithm attack on 'double images encryption method with resistance against the special attack based on an asymmetric algorithm'

    NASA Astrophysics Data System (ADS)

    Zhang, Chenggong; He, Wenqi; Cai, Zewei; Peng, Xiang

    2016-09-01

    A generalized amplitude-phase retrieval algorithm (GAPRA) attack on `double images encryption method with resistance against the special attack based on an asymmetric algorithm' (DIEM) is presented in this paper. The analysis shows that the DIEM is a cascaded cryptosystem, which consist of a joint transform correlator architecture and a phasetruncated Fourier transform scheme. A GAPRA attack is proposed and the potential risk of the cascaded cryptosystems is discussed. By using our method, an attacker could crack high-quality results of the plaintexts. A set of simulation results demonstrate the validity and feasibility of the proposed method.

  16. A two-channel method for retrieval of the Black Sea surface temperature from Landsat-8 measurements

    NASA Astrophysics Data System (ADS)

    Aleskerova, A. A.; Kubryakov, A. A.; Stanichny, S. V.

    2016-12-01

    The present work is devoted to the development of a retrieval algorithm for the sea surface temperature from the two-channel measurements of the Landsat-8 satellite on the basis of a comparison with MODIS satellite data. The algorithm makes it possible to reconstruct the surface temperature with a 100 m resolution, which enables analysis of the spatial structure of various phenomena on the ocean surface at small scales (upwelling, submesoscale vortices, etc.). The magnitude of the standard deviation between the reconstructed temperature and the temperature derived from the MODIS satellite is 0.58°C. The sources of inconsistency between temperatures retrieved from the MODIS and Landsat-8 measurements were investigated: diurnal temperature variation, which leads to an increase in the standard deviation in the summer period because of the mismatch in time ( 2 hours) between measurements; the presence of specific bands in the Landsat data, which is probably related to instrumental errors of the device.

  17. Human factors analysis and design methods for nuclear waste retrieval systems: Human factors design methodology and integration plan

    NASA Astrophysics Data System (ADS)

    Casey, S. M.

    1980-06-01

    The nuclear waste retrieval system intended to be used for the removal of storage canisters (each canister containing a spent fuel rod assembly) located in an underground salt bed depository is discussed. The implementation of human factors engineering principles during the design and construction of the retrieval system facilities and equipment is reported. The methodology is structured around a basic system development effort involving preliminary development, equipment development, personnel subsystem development, and operational test and evaluation. Examples of application of the techniques in the analysis of human tasks, and equipment required in the removal of spent fuel canisters is provided. The framework for integrating human engineering with the rest of the system development effort is documented.

  18. Snow depth and snow cover retrieval from FengYun3B microwave radiation imagery based on a snow passive microwave unmixing method in Northeast China

    NASA Astrophysics Data System (ADS)

    Gu, Lingjia; Ren, Ruizhi; Zhao, Kai; Li, Xiaofeng

    2014-01-01

    The precision of snow parameter retrieval is unsatisfactory for current practical demands. The primary reason is because of the problem of mixed pixels that are caused by low spatial resolution of satellite passive microwave data. A snow passive microwave unmixing method is proposed in this paper, based on land cover type data and the antenna gain function of passive microwaves. The land cover type of Northeast China is partitioned into grass, farmland, bare soil, forest, and water body types. The component brightness temperatures (CBT), namely unmixed data, with 1 km data resolution are obtained using the proposed unmixing method. The snow depth determined by the CBT and three snow depth retrieval algorithms are validated through field measurements taken in forest and farmland areas of Northeast China in January 2012 and 2013. The results show that the overall of the retrieval precision of the snow depth is improved by 17% in farmland areas and 10% in forest areas when using the CBT in comparison with the mixed pixels. The snow cover results based on the CBT are compared with existing MODIS snow cover products. The results demonstrate that more snow cover information can be obtained with up to 86% accuracy.

  19. A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform.

    PubMed

    Lee, Howon; Kim, Hyoki; Kim, Sungsik; Ryu, Taehoon; Kim, Hwangbeom; Bang, Duhee; Kwon, Sunghoon

    2015-02-02

    Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. DNA clones on next-generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from high-density NGS platforms. Here we propose an enabling technology called 'Sniper Cloning' that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for the full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, Sniper Cloning is a week-long process that produces 5,188 error-free synthetic DNAs in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.

  20. Rapid and Accurate Determination of Lipopolysaccharide O-Antigen Types in Klebsiella pneumoniae with a Novel PCR-Based O-Genotyping Method

    PubMed Central

    Shih, Yun-Jui; Cheong, Cheng-Man; Yi, Wen-Ching

    2015-01-01

    Klebsiella pneumoniae, a Gram-negative bacillus that causes life-threatening infections in both hospitalized patients and ambulatory persons, can be classified into nine lipopolysaccharide (LPS) O-antigen serotypes. The O-antigen type has important clinical and epidemiological significance. However, K. pneumoniae O serotyping is cumbersome, and the reagents are not commercially available. To overcome the limitations of conventional serotyping methods, we aimed to create a rapid and accurate PCR method for K. pneumoniae O genotyping. We sequenced the genetic determinants of LPS O antigen from serotypes O1, O2a, O2ac, O3, O4, O5, O8, O9, and O12. We established a two-step genotyping scheme, based on the two genomic regions associated with O-antigen biosynthesis. The first set of PCR primers, which detects alleles at the wzm-wzt loci of the wb gene cluster, distinguishes between O1/O2, O3, O4, O5, O8, O9, and O12. The second set of PCR primers, which detects alleles at the wbbY region, further differentiates between O1, O2a, and O2ac. We verified the specificity of O genotyping against the O-serotype reference strains. We then tested the sensitivity and specificity of O genotyping in K. pneumoniae, using the 56 K-serotype reference strains with known O serotypes determined by an inhibition enzyme-linked immunosorbent assay (iELISA). There is a very good correlation between the O genotypes and classical O serotypes. Three discrepancies were observed and resolved by nucleotide sequencing—all in favor of O genotyping. The PCR-based O genotyping, which can be easily performed in clinical and research microbiology laboratories, is a rapid and accurate method for determining the LPS O-antigen types of K. pneumoniae isolates. PMID:26719438

  1. [Detection of Aspergillus antigen galactomannan using ELISA method: validation of the performances of the method for accreditation].

    PubMed

    Kauffmann-Lacroix, C; Arvier, M; Charron, M; Rodier, M-H; Vassault, A

    2013-03-01

    Diagnosis of invasive aspergillosis for patients with high risk of infection is based on the monitoring of Aspergillus antigenemia assessed by the detection of galactomannan in serum by a sandwich-type ELISA (Biorad(®)). The validation of the method was displayed according to the guide COFRAC SH GTA 04. The internal quality control system settled, involves two quality control samples made of pools of sera (negative and positive). The repeatability of the measurements, as estimated by the coefficients of variation (CV), obtained by two different technicians was found from 9 to 13.7% for the positive control. The CV of the negative control, for which the provider indicates it is not useful in the analytical process, was found from 7.1 to 30%. In our experience it could be an indicator of environmental contamination. The evaluation of the intermediary fidelity was 15.7% for the positive control and 22.5% for the negative one. In the lack of reference material (International Standard) and recommendation from scientific societies, performances obtained will be discussed according to the results reported in the technical form of the supplier and those obtained by 39 laboratories participating in the only available external quality assessment program organized in France by ProBioQual(®) where the CV of reproducibility are 44.7% of unit (mean index 0.131) for the negative control and 18% (mean index 1.089) for the positive one in 2011.

  2. Flexible montage retrieval for image data

    NASA Astrophysics Data System (ADS)

    Sakamoto, Hiroaki; Suzuki, Hideharu; Uemori, Akira

    1994-04-01

    Academic and photo libraries require the reuse of images. If the database is large, similarity retrieval and retrieval by sketch are effective. However, similarity retrieval can retrieve images that are similar to only one key image. Retrieval by sketch can retrieve images that are similar to the sketch, but it is not always easy to make a sketch sufficiently similar to the desired image. To overcome these limitations, we propose a new flexible montage retrieval method (FMR) that allows the user to retrieve images by appropriately combining characteristics of the key images in the same way that a montage picture of a criminal is constructed. In FMR, the user selects one or more key images and specifies areas (called `key regions') manually or by using image manipulating tools. He then indicates the retrieval conditions, for example, the requirement that the image to be retrieved includes specified areas. This paper describes an FMR algorithm that uses a color histogram and texture to quantify the similarity between a key region and an image in the database. The effectiveness of FMR is then statistically demonstrated by comparing its correct retrieval rate with that of retrieval by sketch. The retrieval rate is calculated from the result of retrieving the images that include a specified sky image from among more than 150 images containing various sky images.

  3. New Methods for Retrieval of Chlorophyll Red Fluorescence from Hyperspectral Satellite Instruments: Simulations and Application to GOME-2 and SCIAMACHY

    NASA Technical Reports Server (NTRS)

    Joiner, Joanna; Yoshida, Yasuko; Guanter, Luis; Middleton, Elizabeth M.

    2016-01-01

    Global satellite measurements of solar-induced fluorescence (SIF) from chlorophyll over land and ocean have proven useful for a number of different applications related to physiology, phenology, and productivity of plants and phytoplankton. Terrestrial chlorophyll fluorescence is emitted throughout the red and far-red spectrum, producing two broad peaks near 683 and 736nm. From ocean surfaces, phytoplankton fluorescence emissions are entirely from the red region (683nm peak). Studies using satellite-derived SIF over land have focused almost exclusively on measurements in the far red (wavelengths greater than 712nm), since those are the most easily obtained with existing instrumentation. Here, we examine new ways to use existing hyperspectral satellite data sets to retrieve red SIF (wavelengths less than 712nm) over both land and ocean. Red SIF is thought to provide complementary information to that from the far red for terrestrial vegetation. The satellite instruments that we use were designed to make atmospheric trace-gas measurements and are therefore not optimal for observing SIF; they have coarse spatial resolution and only moderate spectral resolution (0.5nm). Nevertheless, these instruments, the Global Ozone Monitoring Instrument 2 (GOME-2) and the SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY), offer a unique opportunity to compare red and far-red terrestrial SIF at regional spatial scales. Terrestrial SIF has been estimated with ground-, aircraft-, or satellite-based instruments by measuring the filling-in of atmospheric andor solar absorption spectral features by SIF. Our approach makes use of the oxygen (O2) gamma band that is not affected by SIF. The SIF-free O2 gamma band helps to estimate absorption within the spectrally variable O2 B band, which is filled in by red SIF. SIF also fills in the spectrally stable solar Fraunhofer lines (SFLs) at wavelengths both inside and just outside the O2 B band, which further helps

  4. Label-free and real-time detection of antigen-antibody interactions by Oblique-incidence Reflectivity Difference (OIRD) method

    NASA Astrophysics Data System (ADS)

    He, LiPing; Sun, Yue; Dai, Jun; Wang, JingYi; Lü, HuiBin; Wang, ShuFang; Jin, KuiJuan; Zhou, YueLiang; Yang, GuoZhen

    2012-09-01

    We label-free and real-time detected three interaction processes of antigen-antibodies, Human Immunoglobulin G (IgG), Rabbit IgG, and Mouse IgG as the targets, and Goat Anti-human IgG, Goat Anti-rabbit IgG, and Goat Anti-mouse IgG as the probe, by the Oblique-incidence Reflectivity Difference (OIRD) method. The interaction dynamic curves of the OIRD signal, corresponding to the interaction processes of antigen-antibodies, are generated. The reaction times from beginning to equilibrium state are about 1800, 900, and 1200 s for Human IgG, Rabbit IgG, and Mouse IgG, respectively. The experimental results demonstrate that the OIRD method not only can distinguish biomolecular interactions, but also can be used in real-time detection of interactions and dynamic processes of biomolecules.

  5. Retrieving the relief of a low-roughness surface using a two-step interferometric method with blind phase shift of a reference wave

    NASA Astrophysics Data System (ADS)

    Muravsky, Leonid I.; Kmet', Arkady B.; Voronyak, Taras I.

    2012-11-01

    A two-step interferometric method with blind phase shift of a reference wave for surface relief retrieval is considered. The possibility of using this method to reconstruct a macrorelief and microrelief of low-roughness surfaces is studied. Computer simulations have testified to the possibility of obtaining a reliable low-noise reconstruction of a low-roughness surface macrorelief and microrelief with standard deviation of the roughness heights up to λ/10 by using the developed interferogram-processing algorithm. The simulations have shown that the correlation approach, which is used to determine the reference wave blind phase shift, is more suitable for a rough surface than for a smooth one and the increase of surface roughness leads to a sharp decrease of error in comparison with that for a smooth surface. The experiment-based verification of the performance of the proposed interferometric method has been done using an experiment setup based on a Twyman-Green interferometer. Peculiarities of choosing the sampling interval for a rough surface recording are discussed. The experimental results that were obtained virtually coincided with the computer simulation results, proving the feasibility of retrieving both smooth and low-roughness surfaces by the considered method.

  6. Sensitivities of antigen detection and PCR assays greatly increased compared to that of the standard culture method for screening for group B streptococcus carriage in pregnant women.

    PubMed

    Rallu, Fabien; Barriga, Peter; Scrivo, Carole; Martel-Laferrière, Valérie; Laferrière, Céline

    2006-03-01

    Group B streptococcus (GBS) is a major cause of serious infections in neonates. The 2002 revised guidelines of the Centers for Disease Control and Prevention (CDC) for the prevention of perinatal GBS disease recommend that all pregnant women be screened for GBS carriage at between 35 and 37 weeks of gestation and that intrapartum antibiotic prophylaxis be given to carriers. We studied the performances of four different GBS detection assays in the context of antenatal screening. Between May and August 2004, the 605 vaginorectal swab specimens received at our bacteriology laboratory for GBS antenatal detection were tested by the four assays. The standard culture method was done according to the CDC recommendations. The three experimental assays performed with the growth from the selective enrichment (LIM) broth (Todd-Hewitt broth with 15 mug/ml nalidixic acid and 10 mug/ml colistin) after overnight incubation were a GBS antigen detection assay (PathoDx) and two PCR assays (for cfb and scpB). The most accurate assay was the scpB PCR (sensitivity, 99.6%; specificity, 100%), followed by the cfb PCR (sensitivity, 75.3%; specificity, 100%), GBS antigen detection (sensitivity, 57.3%; specificity, 99.5%), and standard culture (sensitivity, 42.3%; specificity, 100%). The GBS antigen detection assay was found to be more sensitive than the standard culture method, and moreover, the assay has a low cost and is easy to perform in all obstetrical centers which have access to the most basic of diagnostic microbiology services. We believe that antigen detection on incubated LIM broth should replace the standard culture method for screening for GBS carriage at 35 to 37 weeks of gestation. The impact of the greater sensitivities of PCR assays on the diminution of neonatal GBS infections remains to be demonstrated.

  7. Tangent height registration method for the Version 1.4 data retrieval algorithm of the solar occultation sensor ILAS-II.

    PubMed

    Tanaka, Tomoaki; Nakajima, Hideaki; Sugita, Takafumi; Ejiri, Mitsumu K; Irie, Hitoshi; Saitoh, Naoko; Terao, Yukio; Kawasaki, Hiroyuki; Usami, Masatoshi; Yokota, Tatsuya; Kobayashi, Hirokazu; Sasano, Yasuhiro

    2007-10-10

    The Improved Limb Atmospheric Spectrometer-II (ILAS-II) is a satellite-borne solar occultation sensor onboard the Advanced Earth Observing Satellite-II (ADEOS-II). The ILAS-II succeeded the ILAS. The ILAS-II used four grating spectrometers to observe vertical profiles of gas volume mixing ratios of trace constituents and was also equipped with a Sun-edge sensor to determine tangent heights geometrically with high precision. The accuracy of gas volume mixing ratios depends on the accuracy of the tangent height determination. The combination method is a tangent height registration method that was developed to give appropriate tangent heights for the ILAS-II Version 1.4 data retrieval algorithm. This study describes the method used in the ILAS-II Version 1.4 retrieval algorithm to register tangent heights. The root-sum-square total random error is estimated to be 30 m, and the total systematic error is 180 m at an altitude of 30 km. The influence of the tangent height errors on the vertical profiles of gas volume mixing ratios in ILAS-II Version 1.4 is estimated by using the relative difference. The relative difference for each species is within 7% (20%) for an altitude shift of +/-100 m(+/-300 m).

  8. Evaluating the utility of satellite soil moisture retrievals over irrigated areas and the ability of land data assimilation methods to correct for unmodeled processes

    NASA Astrophysics Data System (ADS)

    Kumar, S. V.; Peters-Lidard, C. D.; Santanello, J. A.; Reichle, R. H.; Draper, C. S.; Koster, R. D.; Nearing, G.; Jasinski, M. F.

    2015-11-01

    Earth's land surface is characterized by tremendous natural heterogeneity and human-engineered modifications, both of which are challenging to represent in land surface models. Satellite remote sensing is often the most practical and effective method to observe the land surface over large geographical areas. Agricultural irrigation is an important human-induced modification to natural land surface processes, as it is pervasive across the world and because of its significant influence on the regional and global water budgets. In this article, irrigation is used as an example of a human-engineered, often unmodeled land surface process, and the utility of satellite soil moisture retrievals over irrigated areas in the continental US is examined. Such retrievals are based on passive or active microwave observations from the Advanced Microwave Scanning Radiometer for the Earth Observing System (AMSR-E), the Advanced Microwave Scanning Radiometer 2 (AMSR2), the Soil Moisture Ocean Salinity (SMOS) mission, WindSat and the Advanced Scatterometer (ASCAT). The analysis suggests that the skill of these retrievals for representing irrigation effects is mixed, with ASCAT-based products somewhat more skillful than SMOS and AMSR2 products. The article then examines the suitability of typical bias correction strategies in current land data assimilation systems when unmodeled processes dominate the bias between the model and the observations. Using a suite of synthetic experiments that includes bias correction strategies such as quantile mapping and trained forward modeling, it is demonstrated that the bias correction practices lead to the exclusion of the signals from unmodeled processes, if these processes are the major source of the biases. It is further shown that new methods are needed to preserve the observational information about unmodeled processes during data assimilation.

  9. Evaluating the utility of satellite soil moisture retrievals over irrigated areas and the ability of land data assimilation methods to correct for unmodeled processes

    NASA Astrophysics Data System (ADS)

    Kumar, S. V.; Peters-Lidard, C. D.; Santanello, J. A.; Reichle, R. H.; Draper, C. S.; Koster, R. D.; Nearing, G.; Jasinski, M. F.

    2015-06-01

    The Earth's land surface is characterized by tremendous natural heterogeneity and human engineered modifications, both of which are challenging to represent in land surface models. Satellite remote sensing is often the most practical and effective method to observe the land surface over large geographical areas. Agricultural irrigation is an important human induced modifications to natural land surface processes, as it is pervasive across the world and because of its significant influence on the regional and global water budgets. In this article, irrigation is used as an example of a human engineered, unmodeled land surface process, and the utility of satellite soil moisture retrievals over irrigated areas in the continental US is examined. Such retrievals are based on passive or active microwave observations from the Advanced Microwave Scanning Radiometer for the Earth Observing System (AMSR-E), the Advanced Microwave Scanning Radiometer 2 (AMSR2), the Soil Moisture Ocean Salinity (SMOS) mission, WindSat and the Advanced Scatterometer (ASCAT). The analysis suggests that the skill of these retrievals for representing irrigation artifacts is mixed, with ASCAT based products somewhat more skillful than SMOS and AMSR2 products. The article then examines the suitability of typical bias correction strategies in current land data assimilation systems when unmodeled processes dominate the bias between the model and the observations. Using a suite of synthetic experiments that includes bias correction strategies such as quantile mapping and trained forward modeling, it is demonstrated that the bias correction practices lead to the exclusion of the signals from unmodeled processes, if these processes are the major source of the biases. It is further shown that new methods are needed to preserve the observational information about unmodeled processes during data assimilation.

  10. Evaluating the Utility of Satellite Soil Moisture Retrievals over Irrigated Areas and the Ability of Land Data Assimilation Methods to Correct for Unmodeled Processes

    NASA Technical Reports Server (NTRS)

    Kumar, S. V.; Peters-Lidard, C. D.; Santanello, J. A.; Reichle, R. H.; Draper, C. S.; Koster, R. D.; Nearing, G.; Jasinski, M. F.

    2015-01-01

    Earth's land surface is characterized by tremendous natural heterogeneity and human-engineered modifications, both of which are challenging to represent in land surface models. Satellite remote sensing is often the most practical and effective method to observe the land surface over large geographical areas. Agricultural irrigation is an important human-induced modification to natural land surface processes, as it is pervasive across the world and because of its significant influence on the regional and global water budgets. In this article, irrigation is used as an example of a human-engineered, often unmodeled land surface process, and the utility of satellite soil moisture retrievals over irrigated areas in the continental US is examined. Such retrievals are based on passive or active microwave observations from the Advanced Microwave Scanning Radiometer for the Earth Observing System (AMSR-E), the Advanced Microwave Scanning Radiometer 2 (AMSR2), the Soil Moisture Ocean Salinity (SMOS) mission, WindSat and the Advanced Scatterometer (ASCAT). The analysis suggests that the skill of these retrievals for representing irrigation effects is mixed, with ASCAT-based products somewhat more skillful than SMOS and AMSR2 products. The article then examines the suitability of typical bias correction strategies in current land data assimilation systems when unmodeled processes dominate the bias between the model and the observations. Using a suite of synthetic experiments that includes bias correction strategies such as quantile mapping and trained forward modeling, it is demonstrated that the bias correction practices lead to the exclusion of the signals from unmodeled processes, if these processes are the major source of the biases. It is further shown that new methods are needed to preserve the observational information about unmodeled processes during data assimilation.

  11. Development of a streptavidin-anti-carcinoembryonic antigen antibody, radiolabeled biotin pretargeting method for radioimmunotherapy of colorectal cancer. Reagent development.

    PubMed

    Karacay, H; Sharkey, R M; Govindan, S V; McBride, W J; Goldenberg, D M; Hansen, H J; Griffiths, G L

    1997-01-01

    With pretargeting, radioisotope delivery to tumor is decoupled from the long antibody localization process, and this can increase tumor:blood ratios dramatically. Several reagents were prepared for each step of a "two-step" pretargeting method, and their properties were investigated. For pretargeting tumor, streptavidin-monoclonal antibody (StAv-mab) conjugates were prepared by cross-linking sulfo-SMCC-derivatized streptavidin to a free thiol (SH) group on MN-14 [a high-affinity anti-carcinoembryonic antigen (CEA) mab]. Thiolated mabs were generated either by reaction of 2-iminothiolane (2-IT) with mab lysine residues or by reduction of mab disulfide bonds with (2-mercaptoethyl)amine (MEA). Both procedures gave protein-protein conjugates isolated in relatively low yields (20-25%) after preparative size-exclusion (SE) chromatography purification with conservative peak collection. Both StAv-MN-14 conjugates retained their ability to bind to CEA, to an anti-idiotypic antibody to MN-14 (WI2), and to biotin, as demonstrated by SE-HPLC. Two clearing agents, WI2 mab and a biotin-human serum albumin (biotin-HSA) conjugate, were developed to remove excess circulating StAv-MN-14 conjugates in animals. Both clearing proteins were also modified with galactose residues, introduced using an activated thioimidate derivative, to produce clearing agents which would clear rapidly and clear primary mab rapidly. At least 14 galactose residues on WI2 were required to reduce blood levels to 5.9 +/- 0.7% ID/g in 1 h. Faster blood clearance (0.7 +/- 0.2% ID/g) was observed in 1 h using 44 galactose units per WI2. For the delivery of radioisotope to tumor, several biotinylated conjugates consisting of biotin, a linker, and a chelate were prepared. Conjugates showed good in vitro and in vivo stability when D-amino acid peptides were used as linkers, biotin-peptide-DOTA-indium-111 had a slightly longer blood circulation time (0.09 +/- 0.02% ID/g in 1 h) than biotin-peptide-DTPA-indium-111 (0

  12. Significance of specific IgG against sensitizing antigens in extrinsic allergic alveolitis: serological methods in EAA.

    PubMed

    Sterclova, M; Vasakova, M; Metlicka, M

    2011-01-01

    The aim of our study is to find differences in IgG in sera of potentially exposed and nonexposed individuals and to detect differences in concentrations of specific serum IgG among subjects with and without EAA. Seventy-two patients being followed for suspected interstitial lung disease were included. Specific IgG in sera were established by ImmunoCAP. Serum concentrations of Aspergillus fumigatus, Candida albicans IgG and mixture of moulds IgG were higher in subjects with exposure to relevant inhalation antigens (p<0.05). Patients exposed to parrot and mammal hair mixture had higher serum concentration of specific IgG (p<0.05). Subjects without exposure to mites had lower serum IgG to Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras and Glycophagus domesticus (p<0.05). Higher concentration of serum specific IgG may show previous exposure to this antigen. Even though mite specific IgG are not commonly tested in EAA patients, we suggest their immunomodulatory activity may influence susceptibility to other inhalation antigens.

  13. A numerical method for retrieving high oxygen isotope temperatures from plutonic igneous rocks: An example from the Laramie Anorthosite Complex, Wyoming, USA

    SciTech Connect

    Farquhar, J.; Chacko, T. . Dept. of Geology); Frost, B.R. )

    1992-01-01

    The Sybille Pit is a late-stage magnetite-ilmenite-plagioclase-bearing differentiate of the Laramie Anorthosite with a wide range of grain sizes and modal mineralogy. This variability makes Sybille an ideal locality in which to study the factors that affect isotopic thermometry in plutonic environments. The authors have developed a numerical model based on isotope exchange trajectories that retrieves close to magmatic temperatures for samples from Sybille. This method is based on the premise that hand sample-scale sub-systems close to exchange with each other at temperatures that exceed those of the constituent minerals. The temperature of hand-sample scale closure is retrieved by back calculating the isotope exchange trajectories to the temperature at which two samples with widely different model compositions are in isotopic equilibrium. Application of these methods to samples from Sybille provides promising results. Whereas conventional isotopic thermometry of individual samples yields a wide range of temperatures ([approximately]600 to > 1000 C) depending on the mineral-pair chosen, application of this numerical model to multiple samples yields temperatures of 1,070 [+-] 100 C which corresponds closely to the inferred solidus for these rocks.

  14. A Framework Based on 2-D Taylor Expansion for Quantifying the Impacts of Subpixel Reflectance Variance and Covariance on Cloud Optical Thickness and Effective Radius Retrievals Based on the Bispectral Method

    NASA Technical Reports Server (NTRS)

    Zhang, Z.; Werner, F.; Cho, H.-M.; Wind, G.; Platnick, S.; Ackerman, A. S.; Di Girolamo, L.; Marshak, A.; Meyer, K.

    2016-01-01

    The bispectral method retrieves cloud optical thickness (t) and cloud droplet effective radius (re) simultaneously from a pair of cloud reflectance observations, one in a visible or near-infrared (VIS/NIR) band and the other in a shortwave infrared (SWIR) band. A cloudy pixel is usually assumed to be horizontally homogeneous in the retrieval. Ignoring subpixel variations of cloud reflectances can lead to a significant bias in the retrieved t and re. In the literature, the retrievals of t and re are often assumed to be independent and considered separately when investigating the impact of subpixel cloud reflectance variations on the bispectral method. As a result, the impact on t is contributed only by the subpixel variation of VIS/NIR band reflectance and the impact on re only by the subpixel variation of SWIR band reflectance. In our new framework, we use the Taylor expansion of a two-variable function to understand and quantify the impacts of subpixel variances of VIS/NIR and SWIR cloud reflectances and their covariance on the t and re retrievals. This framework takes into account the fact that the retrievals are determined by both VIS/NIR and SWIR band observations in a mutually dependent way. In comparison with previous studies, it provides a more comprehensive understanding of how subpixel cloud reflectance variations impact the t and re retrievals based on the bispectral method. In particular, our framework provides a mathematical explanation of how the subpixel variation in VIS/NIR band influences the re retrieval and why it can sometimes outweigh the influence of variations in the SWIR band and dominate the error in re retrievals, leading to a potential contribution of positive bias to the re retrieval. We test our framework using synthetic cloud fields from a large-eddy simulation and real observations from Moderate Resolution Imaging Spectroradiometer. The predicted results based on our framework agree very well with the numerical simulations. Our

  15. Practical quantum retrieval games

    NASA Astrophysics Data System (ADS)

    Arrazola, Juan Miguel; Karasamanis, Markos; Lütkenhaus, Norbert

    2016-06-01

    Complex cryptographic protocols are often constructed from simpler building blocks. In order to advance quantum cryptography, it is important to study practical building blocks that can be used to develop new protocols. An example is quantum retrieval games (QRGs), which have broad applicability and have already been used to construct quantum money schemes. In this work, we introduce a general construction of quantum retrieval games based on the hidden matching problem and show how they can be implemented in practice using available technology. More precisely, we provide a general method to construct (1-out-of-k ) QRGs, proving that their cheating probabilities decrease exponentially in k . In particular, we define QRGs based on coherent states of light, which can be implemented even in the presence of experimental imperfections. Our results constitute a tool in the arsenal of the practical quantum cryptographer.

  16. Iodine-filter-based mobile Doppler lidar to make continuous and full-azimuth-scanned wind measurements: data acquisition and analysis system, data retrieval methods, and error analysis.

    PubMed

    Wang, Zhangjun; Liu, Zhishen; Liu, Liping; Wu, Songhua; Liu, Bingyi; Li, Zhigang; Chu, Xinzhao

    2010-12-20

    An incoherent Doppler wind lidar based on iodine edge filters has been developed at the Ocean University of China for remote measurements of atmospheric wind fields. The lidar is compact enough to fit in a minivan for mobile deployment. With its sophisticated and user-friendly data acquisition and analysis system (DAAS), this lidar has made a variety of line-of-sight (LOS) wind measurements in different operational modes. Through carefully developed data retrieval procedures, various wind products are provided by the lidar, including wind profile, LOS wind velocities in plan position indicator (PPI) and range height indicator (RHI) modes, and sea surface wind. Data are processed and displayed in real time, and continuous wind measurements have been demonstrated for as many as 16 days. Full-azimuth-scanned wind measurements in PPI mode and full-elevation-scanned wind measurements in RHI mode have been achieved with this lidar. The detection range of LOS wind velocity PPI and RHI reaches 8-10 km at night and 6-8 km during daytime with range resolution of 10 m and temporal resolution of 3 min. In this paper, we introduce the DAAS architecture and describe the data retrieval methods for various operation modes. We present the measurement procedures and results of LOS wind velocities in PPI and RHI scans along with wind profiles obtained by Doppler beam swing. The sea surface wind measured for the sailing competition during the 2008 Beijing Olympics is also presented. The precision and accuracy of wind measurements are estimated through analysis of the random errors associated with photon noise and the systematic errors introduced by the assumptions made in data retrieval. The three assumptions of horizontal homogeneity of atmosphere, close-to-zero vertical wind, and uniform sensitivity are made in order to experimentally determine the zero wind ratio and the measurement sensitivity, which are important factors in LOS wind retrieval. Deviations may occur under certain

  17. Interaction Information Retrieval.

    ERIC Educational Resources Information Center

    Dominich, Sandor

    1994-01-01

    Discussion of information retrieval focuses on an Interaction Information Retrieval model in which documents are interconnected; queries and documents are treated in the same way; and retrieval is the result of the interconnection between query and documents. A theoretical mathematical formulation of this type of retrieval is given. (Contains 31…

  18. A critical review of published methods for analysis of red cell antigen-antibody reactions by flow cytometry, and approaches for resolving problems with red cell agglutination.

    PubMed

    Arndt, Patricia A; Garratty, George

    2010-07-01

    Flow cytometry operators often apply familiar white blood cell (WBC) methods when studying red blood cell (RBC) antigens and antibodies. Some WBC methods are not appropriate for RBCs, as the analysis of RBCs requires special considerations, for example, avoidance of agglutination. One hundred seventy-six published articles from 88 groups studying RBC interactions were reviewed. Three fourths of groups used at least one unnecessary WBC procedure for RBCs, and about one fourth did not use any method to prevent/disperse RBC agglutination. Flow cytometric studies were performed to determine the effect of RBC agglutination on results and compare different methods of preventing and/or dispersing agglutination. The presence of RBC agglutinates have been shown to be affected by the type of pipette tip used for mixing RBC suspensions, the number of antigen sites/RBC, the type and concentration of primary antibody, and the type of secondary antibody. For quantitation methods, for example, fetal maternal hemorrhage, the presence of agglutinates have been shown to adversely affect results (fewer fetal D+ RBCs detected).

  19. Invasive brain-machine interfaces: a survey of paralyzed patients’ attitudes, knowledge and methods of information retrieval

    NASA Astrophysics Data System (ADS)

    Lahr, Jacob; Schwartz, Christina; Heimbach, Bernhard; Aertsen, Ad; Rickert, Jörn; Ball, Tonio

    2015-08-01

    Objective. Brain-machine interfaces (BMI) are an emerging therapeutic option that can allow paralyzed patients to gain control over assistive technology devices (ATDs). BMI approaches can be broadly classified into invasive (based on intracranially implanted electrodes) and noninvasive (based on skin electrodes or extracorporeal sensors). Invasive BMIs have a favorable signal-to-noise ratio, and thus allow for the extraction of more information than noninvasive BMIs, but they are also associated with the risks related to neurosurgical device implantation. Current noninvasive BMI approaches are typically concerned, among other issues, with long setup times and/or intensive training. Recent studies have investigated the attitudes of paralyzed patients eligible for BMIs, particularly patients affected by amyotrophic lateral sclerosis (ALS). These studies indicate that paralyzed patients are indeed interested in BMIs. Little is known, however, about the degree of knowledge among paralyzed patients concerning BMI approaches or about how patients retrieve information on ATDs. Furthermore, it is not yet clear if paralyzed patients would accept intracranial implantation of BMI electrodes with the premise of decoding improvements, and what the attitudes of a broader range of patients with diseases such as stroke or spinal cord injury are towards this new kind of treatment. Approach. Using a questionnaire, we surveyed 131 paralyzed patients for their opinions on invasive BMIs and their attitude toward invasive BMI treatment options. Main results. The majority of the patients knew about and had a positive attitude toward invasive BMI approaches. The group of ALS patients was especially open to the concept of BMIs. The acceptance of invasive BMI technology depended on the improvements expected from the technology. Furthermore, the survey revealed that for paralyzed patients, the Internet is an important source of information on ATDs. Significance. Websites tailored to

  20. A persistent scatterer method for retrieving accurate InSAR ground deformation map over vegetation-decorrelated areas

    NASA Astrophysics Data System (ADS)

    Chen, J.; Zebker, H. A.; Knight, R. J.

    2015-12-01

    InSAR is commonly used to measure surface deformation between different radar passes at cm-scale accuracy and m-scale resolution. However, InSAR measurements are often decorrelated due to vegetation growth, which greatly limits high quality InSAR data coverage. Here we present an algorithm for retrieving InSAR deformation measurements over areas with significant vegetation decorrelation through the use of adaptive interpolation between persistent scatterer (PS) pixels, those points at which surface scattering properties do not change much over time and thus decorrelation artifacts are minimal. The interpolation filter restores phase continuity in space and greatly reduces errors in phase unwrapping. We apply this algorithm to process L-band ALOS interferograms acquired over the San Luis Valley, Colorado and the Tulare Basin, California. In both areas, groundwater extraction for irrigation results in land deformation that can be detected using InSAR. We show that the PS-based algorithm reduces the artifacts from vegetation decorrelation while preserving the deformation signature. The spatial sampling resolution achieved over agricultural fields is on the order of hundreds of meters, usually sufficient for groundwater studies. The improved InSAR data allow us further to reconstruct the SBAS ground deformation time series and transform the measured deformation to head levels using the skeletal storage coefficient and time delay constant inferred from a joint InSAR-well data analysis. The resulting InSAR-head and well-head measurements in the San Luis valley show good agreement with primary confined aquifer pumping activities. This case study demonstrates that high quality InSAR deformation data can be obtained over vegetation-decorrrelated region if processed correctly.

  1. An improved method for the detection of cell surface antigens in samples of low viability using flow cytometry.

    PubMed

    Wing, M G; Montgomery, A M; Songsivilai, S; Watson, J V

    1990-01-24

    A high non-specific background fluorescence signal was observed when cell surface antigen analysis was carried out using flow cytometry on a cell sample which contained a high proportion of dead and dying cells. To overcome this problem it was necessary to analyse the cells in three stages. First the intact cells were identified by their forward (FWD) and 90 degree scatter profile. These cells were gated-on, then analysed on the basis of their FWD scatter and propidium iodide (PI) signal, allowing the dead PI positive cells to be gated out. The PI negative cells were then displayed using their 90 degree scatter and fluorescence signals following staining with the irrelevant antibody control. This revealed a population of dead cells, which despite being PI negative, were non-specifically binding antibody molecules. Such multiparameter analysis permitted the successful analysis of cell surface antigens in preparations of low viability by gating out the high background fluorescence associated with dead PI positive and negative cells.

  2. Developing Atmospheric Retrieval Methods for Direct Imaging Spectroscopy of Gas Giants in Reflected Light. I. Methane Abundances and Basic Cloud Properties

    NASA Astrophysics Data System (ADS)

    Lupu, Roxana E.; Marley, Mark S.; Lewis, Nikole; Line, Michael; Traub, Wesley A.; Zahnle, Kevin

    2016-12-01

    Upcoming space-based coronagraphic instruments in the next decade will perform reflected light spectroscopy and photometry of cool directly imaged extrasolar giant planets. We are developing a new atmospheric retrieval methodology to help assess the science return and inform the instrument design for such future missions, and ultimately interpret the resulting observations. Our retrieval technique employs a geometric albedo model coupled with both a Markov chain Monte Carlo Ensemble Sampler (emcee) and a multimodal nested sampling algorithm (MultiNest) to map the posterior distribution. This combination makes the global evidence calculation more robust for any given model and highlights possible discrepancies in the likelihood maps. As a proof of concept, our current atmospheric model contains one or two cloud layers, methane as a major absorber, and a H2-He background gas. This 6-to-9 parameter model is appropriate for Jupiter-like planets and can be easily expanded in the future. In addition to deriving the marginal likelihood distribution and confidence intervals for the model parameters, we perform model selection to determine the significance of methane and cloud detection as a function of expected signal-to-noise ratio in the presence of spectral noise correlations. After internal validation, the method is applied to realistic spectra of Jupiter, Saturn, and HD 99492c, a model observing target. We find that the presence or absence of clouds and methane can be determined with high confidence, while parameter uncertainties are model dependent and correlated. Such general methods will also be applicable to the interpretation of direct imaging spectra of cloudy terrestrial planets.

  3. Energy fluxes retrieval on an Alaskan Arctic and Sub-Arctic vegetation by means MODIS imagery and the DTD method

    NASA Astrophysics Data System (ADS)

    Cristobal, J.; Prakash, A.; Starkenburg, D. P.; Fochesatto, G. J.; Anderson, M. C.; Gens, R.; Kane, D. L.; Kustas, W.; Alfieri, J. G.

    2012-12-01

    Evapotranspiration (ET) plays a significant role in the hydrologic cycle of Arctic and Sub-Arctic basins. Surface-atmosphere exchanges due to ET are estimated from water balance computations to be about 74% of summer precipitation or 50% of annual precipitation. Even though ET is a significant component of the hydrologic cycle in this region, the bulk estimates don't accurately account for spatial and temporal variability due to vegetation type, topography, etc. (Kane and Yang, 2004). Nowadays, remote sensing is the only technology capable of providing the necessary radiometric measurements for the calculation of the ET at global scales and in a feasible economic way, especially in Arctic and Sub-Arctic Alaskan basins with a very sparse network of both meteorological and flux towers. In this work we present the implementation and validation of the Dual-Time-Difference model (Kustas et al., 2001) to retrieve energy fluxes (ET, sensible heat flux, net radiation and soil heat flux) in tundra vegetation in Arctic conditions and in a black spruce (Picea mariana) forest in Sub-Arctic conditions. In order to validate the model in tundra vegetation we used a flux tower from the Imnavait Creek sites of the Arctic Observatory Network (Euskirchen et al. 2012). In the case of the black spruce forest, on September 2011 we installed a flux tower in the University of Alaska Fairbanks north campus that includes an eddy-covariance system as well a net radiometer, air temperature probes, soil heat flux plates, soil moisture sensors and thermistors to fully estimate energy fluxes in the field (see http://www.et.alaska.edu/ for further details). Additionally, in order to upscale energy fluxes into MODIS spatial resolution, a scintillometer was also installed covering 1.2 km across the flux tower. DTD model mainly requires meteorological inputs as well as land surface temperature (LST) and leaf area index (LAI) data, both coming from satellite imagery, at two different times: after

  4. Human liver nucleolar antigens.

    PubMed

    Busch, R K; Busch, H

    1981-10-01

    In an extension of previous studies on the antigens in rat liver nucleoli (R. K. Busch, R. C. Reddy, D. H. Henning, and H. Busch, Proc. Soc. Exp. Biol. Med. 160, 185 (1979); R. K. Busch and H. Busch, Tumori 63, 347 (1977); F. M. Davis, R. K. Busch, L. C. Yeoman, and H. Busch, Cancer Res. 38, 1906 (1978), rabbit antibodies were elicited to human liver nucleoli isolated by the sucrose--Mg2+ method (10). Fluorescent nucleoli were found in liver cryostat sections treated with rabbit anti-human liver nucleolar antibodies followed by fluorescein-conjugated goat anti-rabbit antibodies. In HeLa cells, fluorescence was distributed throughout the nucleus and in a nuclear network but was not localized to the nucleolus. In placental cryostat sections, an overall nuclear fluorescence was observed with some localization to nucleoli. Immunodiffusion analysis revealed two immunoprecipitin bands which appeared to be liver specific. Other immunoprecipitin bands were common to liver, placenta, and HeLa nuclear extracts. Rocket immunoelectrophoresis revealed two liver-specific antigens, one migrating to the cathode and the other to the anode Other rockets exhibited identity to antigens of other nuclear extracts. These results demonstrate the presence of human liver nucleolar-specific antigens which were not found in the HeLa and placental cells.

  5. A Highly Selective and Sensitive Fluorescence Detection Method of Glyphosate Based on an Immune Reaction Strategy of Carbon Dot Labeled Antibody and Antigen Magnetic Beads.

    PubMed

    Wang, Duo; Lin, Bixia; Cao, Yujuan; Guo, Manli; Yu, Ying

    2016-08-03

    A sensitive fluorescence detection method for glyphosate (GLY) was established based on immune reaction. First, carbon dot labeled antibodies (lgG-CDs) which were able to specifically identify glyphosate were prepared with the environmentally friendly carbon dots (CDs) and glyphosate antibody (lgG). lgG-CDs could be used to in situ visualize the distribution of glyphosate in plant tissues. In order to eliminate the effects of excess lgG-CDs on the determination of GLY, antigen magnetic beads Fe3O4-GLY based on magnetic nanoparticles Fe3O4 and glyphosate were constructed and utilized to couple with the excess lgG-CDs. After magnetic separation to remove antigen magnetic beads, there was a linear relationship between the fluorescence intensity of lgG-CDs and the logarithmic concentration of glyphosate in the range of 0.01-80 μg/mL with a detection limit of 8 ng/mL. The method was used for the detection of glyphosate in Pearl River water, tea, and soil samples with satisfactory recovery ratio between 87.4% and 103.7%.

  6. Developing Atmospheric Retrieval Methods for Direct Imaging Spectroscopy of Gas Giants in Reflected Light I: Methane Abundances and Basic Cloud Properties

    NASA Technical Reports Server (NTRS)

    Lupu, R. E.; Marley, M. S.; Lewis, N.; Line, M.; Traub, W.; Zahnle, K.

    2016-01-01

    Reflected light spectroscopy and photometry of cool, directly imaged extrasolar giant planets are expected to be performed in the next decade by space-based telescopes equipped with optical wavelength coronagraphs and integral field spectrographs, such as the Wide-Field Infrared Survey Telescope (WFIRST). We are developing a new atmospheric retrieval methodology to help assess the science return and inform the instrument design for such future missions, and ultimately interpret the resulting observations. Our retrieval technique employs an albedo model coupled with both a Markov chain Monte Carlo Ensemble Sampler (emcee) and a multimodal nested sampling algorithm (MultiNest) to map the posterior distribution. This combination makes the global evidence calculation more robust for any given model, and highlights possible discrepancies in the likelihood maps. Here we apply this methodology to simulated spectra of cool giant planets. As a proof-of-concept, our current atmospheric model contains 1 or 2 cloud layers, methane as a major absorber, and a H2-He background gas. This 6-to-9 parameter model is appropriate for Jupiter-like planets and can be easily expanded in the future. In addition to deriving the marginal likelihood distribution and confidence intervals for the model parameters, we perform model selection to determine the significance of methane and cloud detection as a function of expected signal-to-noise, in the presence of spectral noise correlations. After internal validation, the method is applied to realistic reflected-light spectra of Jupiter, Saturn, and HD 99492 c, a likely observing target. We find that the presence or absence of clouds and methane can be determined with high accuracy, while parameters uncertainties are model-dependent.

  7. Use of In Situ Cloud Condensation Nuclei, Extinction, and Aerosol Size Distribution Measurements to Test a Method for Retrieving Cloud Condensation Nuclei Profiles From Surface Measurements

    NASA Technical Reports Server (NTRS)

    Ghan, Stephen J.; Rissman, Tracey A.; Ellman, Robert; Ferrare, Richard A.; Turner, David; Flynn, Connor; Wang, Jian; Ogren, John; Hudson, James; Jonsson, Haflidi H.; VanReken, Timothy; Flagan, Richard C.; Seinfeld, John H.

    2006-01-01

    If the aerosol composition and size distribution below cloud are uniform, the vertical profile of cloud condensation nuclei (CCN) concentration can be retrieved entirely from surface measurements of CCN concentration and particle humidification function and surface-based retrievals of relative humidity and aerosol extinction or backscatter. This provides the potential for long-term measurements of CCN concentrations near cloud base. We have used a combination of aircraft, surface in situ, and surface remote sensing measurements to test various aspects of the retrieval scheme. Our analysis leads us to the following conclusions. The retrieval works better for supersaturations of 0.1% than for 1% because CCN concentrations at 0.1% are controlled by the same particles that control extinction and backscatter. If in situ measurements of extinction are used, the retrieval explains a majority of the CCN variance at high supersaturation for at least two and perhaps five of the eight flights examined. The retrieval of the vertical profile of the humidification factor is not the major limitation of the CCN retrieval scheme. Vertical structure in the aerosol size distribution and composition is the dominant source of error in the CCN retrieval, but this vertical structure is difficult to measure from remote sensing at visible wavelengths.

  8. Two Methods for Retrieving UV Index for All Cloud Conditions from Sky Imager Products or Total SW Radiation Measurements

    SciTech Connect

    Badosa, Jordi; Calbo, J.; McKenzie, R. L.; Liley, Ben; Gonzalez, J. A.; Forgan, B. W.; Long, Charles N.

    2014-07-01

    In the present study, we assess the cloud effects on UV Index (UVI) and total solar radiation (TR) as a function of cloud cover estimations and sunny conditions (from sky imaging products) as well as of solar zenith angle (SZA). These analyses are undertaken for a southern-hemisphere mid-latitude site where a 10-years dataset is available. It is confirmed that clouds reduce TR more than UV, in particular for obscured Sun conditions, low cloud fraction (< 60%) and large SZA (> 60º). Similarly, clouds enhance TR more than UV, mainly for visible Sun conditions, large cloud fraction and large SZA. Two methods to estimate UVI are developed: 1) from sky imaging cloud cover and sunny conditions, and 2) from TR measurements. Both methods may be used in practical operational applications, although Method 2 shows overall the best performance, since TR allows accounting for cloud optical properties. The mean absolute differences of Method 2 estimations with respect to measured values are 0.17 UVI units (for 1-minute data) and 0.79 Standard Erythemal Dose (SED) units (for daily integrations). Method 1 shows less accurate results but it is still suitable to estimate UVI: mean absolute differences are 0.37 UVI units and 1.6 SED.

  9. Two methods for retrieving UV index for all cloud conditions from sky imager products or total SW radiation measurements.

    PubMed

    Badosa, Jordi; Calbó, Josep; Mckenzie, Richard; Liley, Ben; González, Josep-Abel; Forgan, Bruce; Long, Charles N

    2014-01-01

    Cloud effects on UV Index (UVI) and total solar radiation (TR) as a function of cloud cover and sunny conditions (from sky images) as well as of solar zenith angle (SZA) are assessed. These analyses are undertaken for a southern-hemisphere mid-latitude site where a 10-years dataset is available. It is confirmed that clouds reduce TR more than UV, in particular for obscured Sun conditions, low cloud fraction (<60%) and large SZA (>60°). Similarly, local short-time enhancement effects are stronger for TR than for UV, mainly for visible Sun conditions, large cloud fraction and large SZA. Two methods to estimate UVI are developed: (1) from sky imaging cloud cover and sunny conditions, and (2) from TR measurements. Both methods may be used in practical applications, although Method 2 shows overall the best performance, as TR allows considering cloud optical properties. The mean absolute (relative) differences of Method 2 estimations with respect to measured values are 0.17 UVI units (6.7%, for 1 min data) and 0.79 Standard Erythemal Dose (SED) units (3.9%, for daily integrations). Method 1 shows less accurate results but it is still suitable to estimate UVI: mean absolute differences are 0.37 UVI units (15%) and 1.6 SED (8.0%).

  10. EVA Retriever Demonstration

    NASA Technical Reports Server (NTRS)

    1988-01-01

    The EVA retriever is demonstrated in the Manipulator Development Facility (MDF). The retriever moves on the air bearing table 'searching' for its target, in this case tools 'dropped' by astronauts on orbit.

  11. Kyoto islet isolation method: the optimized one for non-heart-beating donors with highly efficient islet retrieval.

    PubMed

    Okitsu, T; Matsumoto, S; Iwanaga, Y; Noguchi, H; Nagata, H; Yonekawa, Y; Maekawa, T; Tanaka, K

    2005-10-01

    The availability of pancreata for clinical cadaveric islet transplantation is restricted to non-heart-beating donors (NHBDs) in Japan. This forced us to modify the current standard islet isolation protocol that was made up for brain-dead donors and make it suitable for NHBDs. The Kyoto islet isolation method is the one with induction of several steps based on the ideas both already reported literally and invented originally by ourselves. Using this islet isolation method, we isolated islets from 13 human pancreata of NHBDs and transplanted 11 preparations to six type-1 diabetic patients. The rate to meet release criteria of Edmonton protocol was 84.6%. Establishment of this method allowed us to begin a clinical islet transplantation program in Japan and to continue to perform the preparation of islets from NHBDs with high rate to meet the release criteria of the Edmonton protocol.

  12. PICTURES OF A SUSPECT-TRU RETRIEVAL

    SciTech Connect

    GADD, R.R.

    2007-05-24

    Retrieving ''suspect'' transuranic (TRU) waste from the Hanford Site's low-level waste burial grounds is a tall order, due to conditions that have changed as the work progresses. Project personnel developed several new methods for handling the waste that other retrieval operations may find useful. The Waste Retrieval Project is operated by Fluor Hanford, a prime contractor for the U.S. Department of Energy's Richland Operations Office since 1996.

  13. Molecular epidemiology of human hepatitis A virus defined by an antigen-capture polymerase chain reaction method.

    PubMed Central

    Jansen, R W; Siegl, G; Lemon, S M

    1990-01-01

    We describe an immunoaffinity-linked nucleic acid amplification system (antigen-capture/polymerase chain reaction, or AC/PCR) for detection of viruses in clinical specimens and its application to the study of the molecular epidemiology of a picornavirus, hepatitis A virus (HAV). Immunoaffinity capture of virus, synthesis of viral cDNA, and amplification of cDNA by a polymerase chain reaction (PCR) were carried out sequentially in a single reaction vessel. This approach simplified sample preparation and enhanced the specificity of conventional PCR. AC/PCR detected less than one cell culture infectious unit of virus in 80 microliters of sample. Sequencing of AC/PCR reaction products from 34 virus strains demonstrated remarkable conservation at the nucleotide level among most strains but revealed hitherto unsuspected genetic diversity among human isolates. Epidemiologically related strains were identical or closely related in sequence. Virus strains recovered from epidemics of hepatitis A in the United States and Germany were identical in sequence, providing evidence for a previously unrecognized epidemiologic link between these outbreaks. Images PMID:2158093

  14. Microfilm and Information Retrieval.

    ERIC Educational Resources Information Center

    Teplitz, Arthur

    This paper was prepared to provide a frame of reference to the role of microfilm within the information retrieval world and to provide an opportunity for evaluation of the use of microforms for active retrieval applications. The paper discusses the principles of information retrieval, considers subject and classification indexing, and describes…

  15. Immunohistowax processing, a new fixation and embedding method for light microscopy, which preserves antigen immunoreactivity and morphological structures: visualisation of dendritic cells in peripheral organs

    PubMed Central

    Pajak, B.; De Smedt, T.; Moulin, V.; De Trez, C.; Maldonado-Lopez, R.; Vansanten, G.; Briend, E.; Urbain, J.; Leo, O.; Moser, M.

    2000-01-01

    Aims—To describe a new fixation and embedding method for tissue samples, immunohistowax processing, which preserves both morphology and antigen immunoreactivity, and to use this technique to investigate the role of dendritic cells in the immune response in peripheral tissues. Methods—This technique was used to stain a population of specialised antigen presenting cells (dendritic cells) that have the unique capacity to sensitise naive T cells, and therefore to induce primary immune responses. The numbers of dendritic cells in peripheral organs of mice either untreated or injected with live Escherichia coli were compared. Results—Numbers of dendritic cells were greatly decreased in heart, kidney, and intestine after the inoculation of bacteria. The numbers of dendritic cells in the lung did not seem to be affected by the injection of E coli. However, staining of lung sections revealed that some monocyte like cells acquired morphological and phenotypic features of dendritic cells, and migrated into blood vessels. Conclusions—These observations suggest that the injection of bacteria induces the activation of dendritic cells in peripheral organs, where they play the role of sentinels, and/or their movement into lymphoid organs, where T cell priming is likely to occur. Key Words: dendritic cell • Escherichia coli • immunohistochemistry PMID:10961175

  16. Evaluating the Effectiveness of Information Retrieval Systems Using Simulated Queries.

    ERIC Educational Resources Information Center

    Gordon, Michael D.

    1990-01-01

    Describes a simulation method for estimating recall and fallout in a document retrieval system. Earlier research on simulating document retrieval systems is reviewed, examples are presented of the current method, a probabilistic justification of the method is given, theoretical concerns dealing with retrieval precision are discussed, and further…

  17. A versatile method for protein-based antigen bioanalysis in non-clinical pharmacokinetics studies of a human monoclonal antibody drug by an immunoaffinity liquid chromatography-tandem mass spectrometry.

    PubMed

    Onami, Ichio; Ayabe, Miho; Murao, Naoaki; Ishigai, Masaki

    2014-03-21

    A versatile immunoaffinity liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to quantify the total concentration of a protein-based antigen in non-clinical pharmacokinetics (PK) studies of a human monoclonal antibody drug. The method combines using magnetic beads that have been coated with a commercial anti-human Fc region antibody to capture an immune complex of the antigen and antibody drug, with subsequent digestion and quantification of the antigen-derived tryptic peptide via LC-MS/MS. Although a typical immunoassay or an immunoaffinity LC-MS/MS assay requires an antigen-specific antibody that uses a different epitope from the antibody drug, this method requires only a commercial anti-human Fc region antibody. The method was applied to quantify total receptor activator of nuclear factor-κB ligand (RANKL) in the presence of denosumab, a humanized monoclonal antibody (mAb) specific to RANKL. The assay was validated as fit-for-purpose and found to be accurate (<115% interbatch accuracies) and precise (<15%, interbatch coefficient of variation) across a range of 3.13-200ng/mL RANKL. Commercial enzyme-linked immunosorbent assay (ELISA) kit was not able to determine the total RANKL because interference by denosumab decreased recovery. In contrast, the antibody drug had less effect on the LC-MS/MS method. The method now provides a bioanalytical platform for developing other protein-based antigen assays in the early drug stage.

  18. Inter-comparison of four remote sensing based surface energy balance methods to retrieve surface evapotranspiration and water stress of irrigated fields in semi-arid climate

    NASA Astrophysics Data System (ADS)

    Chirouze, J.; Boulet, G.; Jarlan, L.; Fieuzal, R.; Rodriguez, J. C.; Ezzahar, J.; Er-Raki, S.; Bigeard, G.; Merlin, O.; Garatuza-Payan, J.; Watts, C.; Chehbouni, G.

    2013-01-01

    Remotely sensed surface temperature can provide a good proxy for water stress level and is therefore particularly useful to estimate spatially distributed evapotranspiration. Instantaneous stress levels or instantaneous latent heat flux are deduced from the surface energy balance equation constrained by this equilibrium temperature. Pixel average surface temperature depends on two main factors: stress and vegetation fraction cover. Methods estimating stress vary according to the way they treat each factor. Two families of methods can be defined: the contextual methods, where stress levels are scaled on a given image between hot/dry and cool/wet pixels for a particular vegetation cover, and single-pixel methods which evaluate latent heat as the residual of the surface energy balance for one pixel independently from the others. Four models, two contextual (S-SEBI and a triangle method, inspired by Moran et al., 1994) and two single-pixel (TSEB, SEBS) are applied at seasonal scale over a four by four km irrigated agricultural area in semi-arid northern Mexico. Their performances, both at local and spatial standpoints, are compared relatively to energy balance data acquired at seven locations within the area, as well as a more complex soil-vegetation-atmosphere transfer model forced with true irrigation and rainfall data. Stress levels are not always well retrieved by most models, but S-SEBI as well as TSEB, although slightly biased, show good performances. Drop in model performances is observed when vegetation is senescent, mostly due to a poor partitioning both between turbulent fluxes and between the soil/plant components of the latent heat flux and the available energy. As expected, contextual methods perform well when extreme hydric and vegetation conditions are encountered in the same image (therefore, esp. in spring and early summer) while they tend to exaggerate the spread in water status in more homogeneous conditions (esp. in winter).

  19. Method of in situ retrieval of contaminants or other substances using a barrier system and leaching solutions

    DOEpatents

    Nickelson, Reva A.; Walsh, Stephanie; Richardson, John G.; Dick, John R.; Sloan, Paul A.

    2006-12-26

    Processes and methods relating to treating contaminants and collecting desired substances from a zone of interest using subterranean collection and containment barriers. Tubular casings having interlock structures are used to create subterranean barriers for containing and treating buried waste and its effluents. The subterranean barrier includes an effluent collection system. Treatment solutions provided to the zone of interest pass therethrough and are collected by the barrier and treated or recovered, allowing on-site remediation. Barrier components may be used to in the treatment by collecting or removing contaminants or other materials from the zone of interest.

  20. A Variational Method to Retrieve the Extinction Profile in Liquid Clouds Using Multiple Field-of-View Lidar

    NASA Technical Reports Server (NTRS)

    Pounder, Nicola L.; Hogan, Robin J.; Varnai, Tamas; Battaglia, Alessandro; Cahalan, Robert F.

    2011-01-01

    While liquid clouds playa very important role in the global radiation budget, it's been very difficult to remotely determine their internal cloud structure. Ordinary lidar instruments (similar to radars but using visible light pulses) receive strong signals from such clouds, but the information is limited to a thin layer near the cloud boundary. Multiple field-of-view (FOV) lidars offer some new hope as they are able to isolate photons that were scattered many times by cloud droplets and penetrated deep into a cloud before returning to the instrument. Their data contains new information on cloud structure, although the lack of fast simulation methods made it challenging to interpret the observations. This paper describes a fast new technique that can simulate multiple-FOV lidar signals and can even estimate the way the signals would change in response to changes in cloud properties-an ability that allows quick refinements in our initial guesses of cloud structure. Results for a hypothetical airborne three-FOV lidar suggest that this approach can help determine cloud structure for a deeper layer in clouds, and can reliably determine the optical thickness of even fairly thick liquid clouds. The algorithm is also applied to stratocumulus observations by the 8-FOV airborne "THOR" lidar. These tests demonstrate that the new method can determine the depth to which a lidar provides useful information on vertical cloud structure. This work opens the way to exploit data from spaceborne lidar and radar more rigorously than has been possible up to now.

  1. ARM Cloud Retrieval Ensemble Data Set (ACRED)

    SciTech Connect

    Zhao, C; Xie, S; Klein, SA; McCoy, R; Comstock, JM; Delanoë, J; Deng, M; Dunn, M; Hogan, RJ; Jensen, MP; Mace, GG; McFarlane, SA; O’Connor, EJ; Protat, A; Shupe, MD; Turner, D; Wang, Z

    2011-09-12

    This document describes a new Atmospheric Radiation Measurement (ARM) data set, the ARM Cloud Retrieval Ensemble Data Set (ACRED), which is created by assembling nine existing ground-based cloud retrievals of ARM measurements from different cloud retrieval algorithms. The current version of ACRED includes an hourly average of nine ground-based retrievals with vertical resolution of 45 m for 512 layers. The techniques used for the nine cloud retrievals are briefly described in this document. This document also outlines the ACRED data availability, variables, and the nine retrieval products. Technical details about the generation of ACRED, such as the methods used for time average and vertical re-grid, are also provided.

  2. Scalable ranked retrieval using document images

    NASA Astrophysics Data System (ADS)

    Jain, Rajiv; Oard, Douglas W.; Doermann, David

    2013-12-01

    Despite the explosion of text on the Internet, hard copy documents that have been scanned as images still play a significant role for some tasks. The best method to perform ranked retrieval on a large corpus of document images, however, remains an open research question. The most common approach has been to perform text retrieval using terms generated by optical character recognition. This paper, by contrast, examines whether a scalable segmentation-free image retrieval algorithm, which matches sub-images containing text or graphical objects, can provide additional benefit in satisfying a user's information needs on a large, real world dataset. Results on 7 million scanned pages from the CDIP v1.0 test collection show that content based image retrieval finds a substantial number of documents that text retrieval misses, and that when used as a basis for relevance feedback can yield improvements in retrieval effectiveness.

  3. A novel method to retrieve Aerosol Optical Thickness from high-resolution optical satellite images using an extended version of the Haze Optimized Transform (HOTBAR)

    NASA Astrophysics Data System (ADS)

    Wilson, Robin; Milton, Edward; Nield, Joanna

    2016-04-01

    Aerosol Optical Thickness (AOT) data has many important applications including atmospheric correction of satellite imagery and monitoring of particulate matter air pollution. Current data products are generally available at a kilometre-scale resolution, but many applications require far higher resolutions. For example, particulate matter concentrations vary on a metre-scale, and thus data products at a similar scale are required to provide accurate assessments of particle densities and allow effective monitoring of air quality and analysis of local air quality effects on health. A novel method has been developed which retrieves per-pixel AOT values from high-resolution (~30m) satellite data. This method is designed to work over a wide range of land covers - including both bright and dark surfaces - and requires only standard visible and near-infrared data, making it applicable to a range of data from sensors such as Landsat, SPOT and Sentinel-2. The method is based upon an extension of the Haze Optimized Transform (HOT). The HOT was originally designed for assessing areas of thick haze in satellite imagery by calculating a 'haziness' value for each pixel in an image as the distance from a 'Clear Line' in feature space, defined by the high correlation between visible bands. Here, we adapt the HOT method and use it to provide AOT data instead. Significant extensions include Monte Carlo estimation of the 'Clear Line', object-based correction for land cover, and estimation of AOT from the haziness values through radiative transfer modelling. This novel method will enable many new applications of AOT data that were impossible with previously available low-resolution data, and has the potential to contribute significantly to our understanding of the air quality on health, the accuracy of satellite image atmospheric correction and the role of aerosols in the climate system.

  4. Intercomparison of four remote-sensing-based energy balance methods to retrieve surface evapotranspiration and water stress of irrigated fields in semi-arid climate

    NASA Astrophysics Data System (ADS)

    Chirouze, J.; Boulet, G.; Jarlan, L.; Fieuzal, R.; Rodriguez, J. C.; Ezzahar, J.; Er-Raki, S.; Bigeard, G.; Merlin, O.; Garatuza-Payan, J.; Watts, C.; Chehbouni, G.

    2014-03-01

    Instantaneous evapotranspiration rates and surface water stress levels can be deduced from remotely sensed surface temperature data through the surface energy budget. Two families of methods can be defined: the contextual methods, where stress levels are scaled on a given image between hot/dry and cool/wet pixels for a particular vegetation cover, and single-pixel methods, which evaluate latent heat as the residual of the surface energy balance for one pixel independently from the others. Four models, two contextual (S-SEBI and a modified triangle method, named VIT) and two single-pixel (TSEB, SEBS) are applied over one growing season (December-May) for a 4 km × 4 km irrigated agricultural area in the semi-arid northern Mexico. Their performance, both at local and spatial standpoints, are compared relatively to energy balance data acquired at seven locations within the area, as well as an uncalibrated soil-vegetation-atmosphere transfer (SVAT) model forced with local in situ data including observed irrigation and rainfall amounts. Stress levels are not always well retrieved by most models, but S-SEBI as well as TSEB, although slightly biased, show good performance. The drop in model performance is observed for all models when vegetation is senescent, mostly due to a poor partitioning both between turbulent fluxes and between the soil/plant components of the latent heat flux and the available energy. As expected, contextual methods perform well when contrasted soil moisture and vegetation conditions are encountered in the same image (therefore, especially in spring and early summer) while they tend to exaggerate the spread in water status in more homogeneous conditions (especially in winter). Surface energy balance models run with available remotely sensed products prove to be nearly as accurate as the uncalibrated SVAT model forced with in situ data.

  5. Retrieval of mixed layer height (MLH) from lidar using analytical methods and estimation of MLH growth rates over a tropical site Gadanki

    NASA Astrophysics Data System (ADS)

    Vishnu, R.; Bhavani Kumar, Y.; Sinha, P. R.; Narayana Rao, T.; James Jebaseelan Samuel, E.

    2016-05-01

    A single channel elastic-backscatter lidar system was developed in-house at National Atmospheric Research Laboratory, an institution under Department of Space and Government of India, for studies on boundary layer dynamics during convective periods. The developed lidar system operates at the second harmonic wavelength of Nd: YAG laser and uses biaxial configuration. The lidar system utilizes a mini PMT for detecting laser returns from the atmosphere and operates in the analogue mode of data acquisition. The analogue recorder operates at 20 MHz sampling and uses a 12 bit A to D converter. The lidar system capable to operate at a maximum vertical resolution 7.5 m and 1-sec time sampling. However, in the present study, the lidar was operated with 30 m vertical resolution and 30-sec time sampling to understand the boundary layer dynamics during convective periods. The lidar measurements conducted between January and March 2014 were used in the present study. The laser backscatters obtained at 532 nm wavelength were corrected for noise and range before application of above mentioned analytical methods. This study presents evaluation of mixed layer height (MLH) from lidar using different analytical methods such as gradient, variance and wavelet techniques and presentation of inter-comparison between methods to achieve suitable method for assessment of MLH. The estimated MLH is then compared with the simultaneous radiosonde observations and empirical model values. We computed the MLH growth rates and observed that a significant enhancement was seen during the transition from winter to pre-monsoon period which could be attributed to increased convective activity over the tropical site. We present the lidar measurements and discuss the MLH retrieval and growth rates over Gadanki using lidar measurements.

  6. A Web Information Retrieval System

    NASA Astrophysics Data System (ADS)

    Kim, Tae-Hyun; Park, Dong-Chul; Huh, Woong; Kim, Hyen-Ug; Yoon, Chung-Hwa; Park, Chong-Dae; Woo, Dong-Min; Jeong, Taikyeong; Cho, Il-Hwan; Lee, Yunsik

    An approach for the retrieval of price information from internet sites is applied to real-world application problems in this paper. The Web Information Retrieval System (WIRS) utilizes Hidden Markov Model (HMM) for its powerful capability to process temporal information. HMM is an extremely flexible tool and has been successfully applied to a wide variety of stochastic modeling tasks. In order to compare the prices and features of products from various web sites, the WIRS extracts prices and descriptions of various products within web pages. The WIRS is evaluated with real-world problems and compared with a conventional method and the result is reported in this paper.

  7. The Ecosystem of Information Retrieval

    ERIC Educational Resources Information Center

    Rodriguez-Munoz, Jose-Vicente; Martinez-Mendez, Francisco-Javier; Pastor-Sanchez, Juan-Antonio

    2012-01-01

    Introduction: This paper presents an initial proposal for a formal framework that, by studying the metric variables involved in information retrieval, can establish the sequence of events involved and how to perform it. Method: A systematic approach from the equations of Shannon and Weaver to establish the decidability of information retrieval…

  8. Canopy Water Content retrieval at different scales from empirical and physically-based remote sensing methods in the SMOS VAS cal/val site

    NASA Astrophysics Data System (ADS)

    Camacho de Coca, Fernando

    based on the ground data. Finally, to produce the VWC maps over the whole site, the HR maps have been remapped to the spatial resolution of MODIS MCD43B4 product (1 km). A linear relationship between the SIWSI MODIS index and ground maps has been found, with a correlation coefficient better than 0.9. 2. A coupled version of the radiative transfer models PROSPECT and SAILH was used to derive spatially distributed estimates of CWC variables from CHRIS/PROBA data acquired during the ESA Sen3Exp campaign. Model inversion through a feed forward artificial neural network, was applied to selected bands of the CHRIS sensor. The network was trained using a backpropagation algorithmn based on simulated spectra to relate the canopy variables as independently and simultaneously outputs. Different combinations of spectral bands were evaluated too as input variables. Other network structural properties such as the number of hidden layers, the number of neurons or the activation function have been studied to optimize the results. Finally, a comparison of retrievals from empirical and physically-based methods is conducted, and the performance and limitations of each approach for mapping CWC analyzed.

  9. Retrieving soil surface temperature under snowpack using special sensor microwave/imager brightness temperature in forested areas of Heilongjiang, China: an improved method

    NASA Astrophysics Data System (ADS)

    Zheng, Xingming; Li, Xiaofeng; Jiang, Tao; Ding, Yanling; Wu, Lili; Zhang, Shiyi; Zhao, Kai

    2016-04-01

    Soil surface temperature (Ts) is an important indicator of global temperature change and a key input parameter for retrieving land surface variables using remote sensing techniques. Due to the masking in the thermal infrared band and the scattering in the microwave band of snow, the temperature of soil surfaces covered by snow is difficult to infer from remote sensing data. We attempted to estimate Ts under snow cover using brightness temperature data from the special sensor microwave/imager. Ts under snow cover was underestimated due to the strong scattering effect of snow on upward soil microwave emissions at 37 GHz. The underestimated portion of Ts is related to snow properties, such as depth, grain size, and moisture. Based on the microwave emission model of layered snowpacks, the simulated results revealed a linear relationship between the underestimated Ts and the brightness temperature difference (TBD) at 19 and 37 GHz. When TBDs at 19 and 37 GHz were introduced to the Ts estimation method, accuracy improved, i.e., the root mean square error and bias of the estimated Ts decreased greatly, especially for dry snow. This improvement allows Ts estimation of snow-covered surfaces from 37 GHz microwave brightness temperature.

  10. Exploiting the power law distribution properties of satellite fire radiative power retrievals: A method to estimate fire radiative energy and biomass burned from sparse satellite observations

    NASA Astrophysics Data System (ADS)

    Kumar, S. S.; Roy, D. P.; Boschetti, L.; Kremens, R.

    2011-10-01

    Instantaneous estimates of the power released by fire (fire radiative power, FRP) are available with satellite active fire detection products. The temporal integral of FRP provides an estimate of the fire radiative energy (FRE) that is related linearly to the amount of biomass burned needed by the atmospheric emissions modeling community. The FRE, however, is sensitive to satellite temporal and spatial FRP undersampling due to infrequent satellite overpasses, cloud and smoke obscuration, and failure to detect cool and/or small fires. Satellite FRPs derived over individual burned areas and fires have been observed to exhibit power law distributions. This property is exploited to develop a new way to derive FRE, as the product of the fire duration and the expected FRP value derived from the FRP power law probability distribution function. The method is demonstrated and validated by the use of FRP data measured with a dual-band radiometer over prescribed fires in the United States and by the use of FRP data retrieved from moderate resolution imaging spectroradiometer (MODIS) active-fire detections over Brazilian deforestation and Australian savanna fires. The biomass burned derived using the conventional FRP temporal integration and power law FRE estimation methods is compared with biomass burned measurements (prescribed fires) and available fuel load information reported in the literature (Australian and Brazilian fires). The results indicate that the FRE power law derivation method may provide more reliable burned biomass estimates under sparse satellite FRP sampling conditions and correct for satellite active-fire detection omission errors if the FRP power law distribution parameters and the fire duration are known.

  11. Wellhead bowl protector and retrieving tool

    SciTech Connect

    Young, J.A.

    1991-09-03

    This patent describes improvement in a wellhead protection system including a wear bushing and a retrieving tool. The improvement comprises a wear bushing supported within the wellhead, wherein the wear bushing includes an enlarged upper end having an external support shoulder for engagement with an internal support shoulder formed in the wellhead; wherein the wear bushing further includes an internal circumferential slot intersected by at least one vertically extending slot, the vertical slot extending from the circumferential slot to the upper end of the wear bushing; a retrieving tool having at least one outwardly biased, retractable lug member mounted thereon; and wherein the retrieving tool includes an enlarged portion adapted to be received within the enlarged upper end of the wear bushing. This patent also describes a method of retrieving a wear bushing from a wellhead comprising the steps of: lowering a retrieving tool into the wellhead for locking engagement with the wear bushing; aligning the retrieving tool with the wear bushing for automatically forcing lug members carried by the retrieving tool outwardly into locking engagement with the wear bushing; monitoring drill string weight for determining engagement of the retrieving tool with the wear bushing, wherein a substantial decrease in drill string weight is an indication that the retrieving tool is engaged with the wear bushing; and removing the wear bushing from the wellhead.

  12. Common antigens between hydatid cyst and cancers

    PubMed Central

    Daneshpour, Shima; Bahadoran, Mehran; Hejazi, Seyed Hossein; Eskandarian, Abas Ali; Mahmoudzadeh, Mehdi; Darani, Hossein Yousofi

    2016-01-01

    Background: Different research groups reported a negative correlation between cancers and parasitical infections. As an example, the prevalence of a hydatid cyst among patients with cancer was significantly lower than its prevalence among normal population. Tn antigens exist both in cancer and hydatid cyst. This common antigen may be involved in the effect of parasite on cancer growth. So in this work, common antigens between hydatid cyst and cancers have been investigated. Materials and Methods: Different hydatid cyst antigens including hydatid fluid, laminated and germinal layer antigens, and excretory secretory antigens of protoscolices were run in SDS PAGE and transferred to NCP paper. In western immunoblotting, those antigens were probed with sera of patients with different cancer and also sera of non-cancer patients. Also, cross reaction among excretory secretory products of cancer cells and antisera raised against different hydatid cyst antigen was investigated. Results: In western immunoblotting, antisera raised against laminated and germinal layers of hydatid cyst reacted with excretory secretory products of cancer cells. Also, a reaction was detected between hydatid cyst antigens and sera of patients with some cancers. Conclusion: Results of this work emphasize existence of common antigens between hydatid cyst and cancers. More investigation about these common antigens is recommended. PMID:26962511

  13. Phase retrieval in protein crystallography.

    PubMed

    Liu, Zhong Chuan; Xu, Rui; Dong, Yu Hui

    2012-03-01

    Solution of the phase problem is central to crystallographic structure determination. An oversampling method is proposed, based on the hybrid input-output algorithm (HIO) [Fienup (1982). Appl. Opt. 21, 2758-2769], to retrieve the phases of reflections in crystallography. This method can extend low-resolution structures to higher resolution for structure determination of proteins without additional sample preparation. The method requires an envelope of the protein which divides a unit cell into the density region where the proteins are located and the non-density region occupied by solvents. After a few hundred to a few thousand iterations, the correct phases and density maps are recovered. The method has been used successfully in several cases to retrieve the phases from the experimental X-ray diffraction data and the envelopes of proteins constructed from structure files downloaded from the Protein Data Bank. It is hoped that this method will greatly facilitate the ab initio structure determination of proteins.

  14. Günter tulip filter retrieval experience: predictors of successful retrieval.

    PubMed

    Turba, Ulku Cenk; Arslan, Bulent; Meuse, Michael; Sabri, Saher; Macik, Barbara Gail; Hagspiel, Klaus D; Matsumoto, Alan H; Angle, John F

    2010-08-01

    We report our experience with Günter Tulip filter placement indications, retrievals, and procedural problems, with emphasis on alternative retrieval techniques. We have identified 92 consecutive patients in whom a Günter Tulip filter was placed and filter removal attempted. We recorded patient demographic information, filter placement and retrieval indications, procedures, standard and nonstandard filter retrieval techniques, complications, and clinical outcomes. The mean time to retrieval for those who experienced filter strut penetration was statistically significant [F(1,90) = 8.55, p = 0.004]. Filter strut(s) IVC penetration and successful retrieval were found to be statistically significant (p = 0.043). The filter hook-IVC relationship correlated with successful retrieval. A modified guidewire loop technique was applied in 8 of 10 cases where the hook appeared to penetrate the IVC wall and could not be engaged with a loop snare catheter, providing additional technical success in 6 of 8 (75%). Therefore, the total filter retrieval success increased from 88 to 95%. In conclusion, the Günter Tulip filter has high successful retrieval rates with low rates of complication. Additional maneuvers such as a guidewire loop method can be used to improve retrieval success rates when the filter hook is endothelialized.

  15. Information Retrieval System.

    ERIC Educational Resources Information Center

    Mahle, Jack D., Jr.

    The Fort Detrick Information Retrieval System is a system of computer programs written in COBOL for a CDC 3150 to store and retrieve information about the scientific and technical reports and documents of the Fort Detrick Technical Library. The documents and reports have been abstracted and indexed. This abstract, the subject matter descriptors,…

  16. Natural Language Information Retrieval.

    ERIC Educational Resources Information Center

    Strzalkowski, Tomek

    1995-01-01

    Describes an information retrieval system in which advanced natural language processing is used to enhance the effectiveness of term-based document retrieval by preprocessing the documents; discovering interterm dependencies and build a conceptual hierarchy specific to database domain; and processing the user's natural language requests into…

  17. RETRIEVAL EVENTS EVALUATION

    SciTech Connect

    T. Wilson

    1999-11-12

    The purpose of this analysis is to evaluate impacts to the retrieval concept presented in the Design Analysis ''Retrieval Equipment and Strategy'' (Reference 6), from abnormal events based on Design Basis Events (DBE) and Beyond Design Basis Events (BDBE) as defined in two recent analyses: (1) DBE/Scenario Analysis for Preclosure Repository Subsurface Facilities (Reference 4); and (2) Preliminary Preclosure Design Basis Event Calculations for the Monitored Geologic Repository (Reference 5) The objective of this task is to determine what impacts the DBEs and BDBEs have on the equipment developed for retrieval. The analysis lists potential impacts and recommends changes to be analyzed in subsequent design analyses for developed equipment, or recommend where additional equipment may be needed, to allow retrieval to be performed in all DBE or BDBE situations. This analysis supports License Application design and therefore complies with the requirements of Systems Description Document input criteria comparison as presented in Section 7, Conclusions. In addition, the analysis discusses the impacts associated with not using concrete inverts in the emplacement drifts. The ''Retrieval Equipment and Strategy'' analysis was based on a concrete invert configuration in the emplacement drift. The scope of the analysis, as presented in ''Development Plan for Retrieval Events Evaluation'' (Reference 3) includes evaluation and criteria of the following: Impacts to retrieval from the emplacement drift based on DBE/BDBEs, and changes to the invert configuration for the preclosure period. Impacts to retrieval from the main drifts based on DBE/BDBEs for the preclosure period.

  18. Compact binary hashing for music retrieval

    NASA Astrophysics Data System (ADS)

    Seo, Jin S.

    2014-03-01

    With the huge volume of music clips available for protection, browsing, and indexing, there is an increased attention to retrieve the information contents of the music archives. Music-similarity computation is an essential building block for browsing, retrieval, and indexing of digital music archives. In practice, as the number of songs available for searching and indexing is increased, so the storage cost in retrieval systems is becoming a serious problem. This paper deals with the storage problem by extending the supervector concept with the binary hashing. We utilize the similarity-preserving binary embedding in generating a hash code from the supervector of each music clip. Especially we compare the performance of the various binary hashing methods for music retrieval tasks on the widely-used genre dataset and the in-house singer dataset. Through the evaluation, we find an effective way of generating hash codes for music similarity estimation which improves the retrieval performance.

  19. The rapid generation of recombinant functional monoclonal antibodies from individual, antigen-specific bone marrow-derived plasma cells isolated using a novel fluorescence-based method

    PubMed Central

    Clargo, Alison M; Hudson, Ashley R; Ndlovu, Welcome; Wootton, Rebecca J; Cremin, Louise A; O'Dowd, Victoria L; Nowosad, Carla R; Starkie, Dale O; Shaw, Sophie P; Compson, Joanne E; White, Dominic P; MacKenzie, Brendon; Snowden, James R; Newnham, Laura E; Wright, Michael; Stephens, Paul E; Griffiths, Meryn R; Lawson, Alastair DG; Lightwood, Daniel J

    2014-01-01

    Single B cell technologies, which avoid traditional hybridoma fusion and combinatorial display, provide a means to interrogate the naturally-selected antibody repertoire of immunized animals. Many methods enable the sampling of memory B cell subsets, but few allow for the direct interrogation of the plasma cell repertoire, i.e., the subset of B cells responsible for producing immunoglobulin in serum. Here, we describe the use of a robust and simple fluorescence-based technique, called the fluorescent foci method, for the identification and isolation of antigen-specific IgG-secreting cells, such as plasma cells, from heterogeneous bone marrow preparations. Following micromanipulation of single cells, cognate pairs of heavy and light chain variable region genes were recovered by reverse transcription (RT)-polymerase chain reaction (PCR). During the PCR, variable regions were combined with a promoter fragment and a relevant constant region fragment to produce two separate transcriptionally-active PCR (TAP) fragments that were directly co-transfected into a HEK-293F cell line for recombinant antibody expression. The technique was successfully applied to the generation of a diverse panel of high-affinity, functional recombinant antibodies to human tumor necrosis factor (TNF) receptor 2 and TNF derived from the bone marrow of immunized rabbits and rats, respectively. Progression from a bone marrow sample to a panel of functional recombinant antibodies was possible within a 2-week timeframe. PMID:24423622

  20. Efficient Graffiti Image Retrieval

    SciTech Connect

    Yang, Chunlei; Wong, Pak C.; Ribarsky, William; Fan, Jianping

    2012-07-05

    Research of graffiti character recognition and retrieval, as a branch of traditional optical character recognition (OCR), has started to gain attention in recent years. We have investigated the special challenge of the graffiti image retrieval problem and propose a series of novel techniques to overcome the challenges. The proposed bounding box framework locates the character components in the graffiti images to construct meaningful character strings and conduct image-wise and semantic-wise retrieval on the strings rather than the entire image. Using real world data provided by the law enforcement community to the Pacific Northwest National Laboratory, we show that the proposed framework outperforms the traditional image retrieval framework with better retrieval results and improved computational efficiency.

  1. Evaluation of multi-layer cloud property retrievals from optimal estimation and Bayesian retrieval algorithms

    NASA Astrophysics Data System (ADS)

    Ding, Y.; Yang, P.

    2015-12-01

    Three physical and radiative cloud properties, namely, optical thickness (tau), effective diameter (De), and cloud top height(h) can be simultaneously inferred from IR radiances for multi-layer cloud cases. The retrieval algorithm implementation is based on a computationally efficient radiative transfer model and spaceborne measurements of narrowband infrared (IR) radiances at the top of the atmosphere. This study focuses on the evaluation of the retrieval results derived from two different algorithms, optimal estimation (OE) algorithm and Bayesian retrieval algorithm. Both of the two methods are able to offer comprehensive error analysis and quality flags. The evaluation results can potentially useful for retrieving the multi-layer clouds properties, a research subject that receives little attention. This presentation will discuss the pros and cons of retrieving cloud properties from the aforesaid retrieval algorithms.

  2. Retrievals of heavy ozone with MIPAS

    NASA Astrophysics Data System (ADS)

    Jonkheid, Bastiaan; Röckmann, Thomas; Glatthor, Norbert; Janssen, Christof; Stiller, Gabriele; von Clarmann, Thomas

    2016-12-01

    A method for retrieval of 18O-substituted isotopomers of O3 in the stratosphere with the Michelson Interferometer for Passive Atmospheric Sounding (MIPAS) is presented. Using a smoothing regularisation constraint, volume mixing ratio profiles are retrieved for the main isotopologue and the symmetric and asymmetric isotopomers of singly substituted O3. For the retrieval of the heavy isotopologues, two microwindows in the MIPAS A band (685-970 cm-1) and six in the AB band (1020-1170 cm-1) are used. As the retrievals are performed as perturbations on the previously retrieved a priori profiles, the vertical resolution of the individual isotopomer profiles is very similar, which is important when calculating the ratio between two isotopomers. The performance of the method is evaluated using 1044 vertical profiles recorded with MIPAS on 1 July 2003. The mean values are separated by latitude bands, along with estimates of their uncertainties. The asymmetric isotopomer shows a mean enrichment of ˜ 8 %, with a vertical profile that increases up to 33 km and decreases at higher altitudes. This decrease with altitude is a robust result that does not depend on retrieval settings, and it has not been reported clearly in previously published datasets. The symmetric isotopomer is considerably less enriched, with mean values around 3 % and with a large spread. In individual retrievals the uncertainty of the enrichment is dominated by the measurement noise (2-4 %), which can be reduced by averaging multiple retrievals; systematic uncertainties linked to the retrieval are generally small at ˜ 0.5 %, but this is likely underestimated because the uncertainties in key spectroscopic parameters are unknown. The variabilities in the retrieval results are largest for the Southern Hemisphere.

  3. Topological Aspects of Information Retrieval.

    ERIC Educational Resources Information Center

    Egghe, Leo; Rousseau, Ronald

    1998-01-01

    Discusses topological aspects of theoretical information retrieval, including retrieval topology; similarity topology; pseudo-metric topology; document spaces as topological spaces; Boolean information retrieval as a subsystem of any topological system; and proofs of theorems. (LRW)

  4. Seminested PCR for Diagnosis of Candidemia: Comparison with Culture, Antigen Detection, and Biochemical Methods for Species Identification

    PubMed Central

    Ahmad, Suhail; Khan, Zaiba; Mustafa, Abu S.; Khan, Zia U.

    2002-01-01

    The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management of patients with hematogenous candidiasis. The presently available culture and biochemical methods for detection and species identification of Candida are time-consuming and lack the required sensitivity and specificity. In this study, we have established a seminested PCR (snPCR) using universal and species-specific primers for detection of Candida species in serum specimens. The universal outer primers amplified the 3′ end of 5.8S ribosomal DNA (rDNA) and the 5′ end of 28S rDNA, including the internally transcribed spacer 2 (ITS2), generating 350- to 410-bp fragments from the four commonly encountered Candida species, viz., C. albicans, C. tropicalis, C. glabrata, and C. parapsilosis. The species-specific primers, complementary to unique sequences within the ITS2 of each test species, amplified species-specific DNA in the reamplification step of the snPCR. The sensitivity of Candida detection by snPCR in spiked serum specimens was close to 1 organism/ml. Evaluation of snPCR for specific identification of Candida species with 76 clinical Candida isolates showed 99% concordant results with the Vitek and/or ID32C yeast identification system. Further evaluation of snPCR for detection of Candida species in sera from culture-proven (n = 12), suspected (n = 16), and superficially colonized (n = 10) patients and healthy subjects (n = 12) showed that snPCR results were consistently negative with sera from healthy individuals and colonized patients. In culture-proven candidemia patients, the snPCR results were in full agreement with blood culture results with respect to both positivity and species identity. In addition, snPCR detected candidemia due to two Candida species in five patients, compared to three by blood culture. In the category of suspected candidemia with negative blood cultures for Candida, nine patients (56%) were positive by sn

  5. Common antigen structures of HL-A antigens

    PubMed Central

    Miyakawa, Y.; Tanigaki, N.; Yagi, Y.; Pressman, D.

    1973-01-01

    Antigenic determinants recognizable by rabbits were found to be present on the molecular fragments (48,000 Daltons) which were obtained by papain-solubilization of the membrane fractions of cultured human lymphoid cells and which carried the HL-A determinants. Results were obtained which suggest that these antigenic determinants are present in common on these molecular fragments carrying HL-A determinants regardless of their HL-A specificity and are restricted to the molecular fragments which carry HL-A determinants. The study was made by use of radioimmune methods involving the binding of radioiodine-labelled soluble HL-A antigen preparations by anti-HL-A alloantisera and by rabbit antisera raised against the membrane fractions of cultured human lymphoid cells. PMID:4119543

  6. Retrieval options study

    SciTech Connect

    Not Available

    1980-03-01

    This Retrieval Options Study is part of the systems analysis activities of the Office of Nuclear Waste Isolation to develop the scientific and technological bases for radioactive waste repositories in various geologic media. The study considers two waste forms, high level waste and spent fuel, and defines various classes of waste retrieval and recovery. A methodology and data base are developed which allow the relative evaluation of retrieval and recovery costs and the following technical criteria: safety; technical feasibility; ease of retrieval; probable intact retrieval time; safeguards; monitoring; criticality; and licensability. A total of 505 repository options are defined and the cost and technical criteria evaluated utilizing a combination of facts and engineering judgments. The repositories evaluated are selected combinations of the following parameters: Geologic Media (salt, granite, basalt, shale); Retrieval Time after Emplacement (5 and 25 years); Emplacement Design (nominal hole, large hole, carbon steel canister, corrosion resistant canister, backfill in hole, nominal sleeves, thick wall sleeves); Emplacement Configuration (single vertical, multiple vertical, single horizontal, multiple horizontal, vaults; Thermal Considerations; (normal design, reduced density, once-through ventilation, recirculated ventilation); Room Backfill; (none, run-of-mine, early, 5 year delay, 25 year delay, decommissioned); and Rate of Retrieval; (same as emplacement, variably slower depending on repository/canister condition).

  7. An integrated content and metadata based retrieval system for art.

    PubMed

    Lewis, Paul H; Martinez, Kirk; Abas, Fazly Salleh; Fauzi, Mohammad Faizal Ahmad; Chan, Stephen C Y; Addis, Matthew J; Boniface, Mike J; Grimwood, Paul; Stevenson, Alison; Lahanier, Christian; Stevenson, James

    2004-03-01

    A new approach to image retrieval is presented in the domain of museum and gallery image collections. Specialist algorithms, developed to address specific retrieval tasks, are combined with more conventional content and metadata retrieval approaches, and implemented within a distributed architecture to provide cross-collection searching and navigation in a seamless way. External systems can access the different collections using interoperability protocols and open standards, which were extended to accommodate content based as well as text based retrieval paradigms. After a brief overview of the complete system, we describe the novel design and evaluation of some of the specialist image analysis algorithms including a method for image retrieval based on sub-image queries, retrievals based on very low quality images and retrieval using canvas crack patterns. We show how effective retrieval results can be achieved by real end-users consisting of major museums and galleries, accessing the distributed but integrated digital collections.

  8. Satellite Retrieval of Aerosol Properties

    NASA Astrophysics Data System (ADS)

    de Leeuw, G.; Robles Gonzalez, C.; Kusmierczyk-Michulec, J.; Decae, R.

    SATELLITE RETRIEVAL of AEROSOL PROPERTIES G. de Leeuw, C. Robles Gonzalez, J. Kusmierczyk-Michulec and R. Decae TNO Physics and Electronics Laboratory, The Hague, The Netherlands; deleeuw@fel.tno.nl Methods to retrieve aerosol properties over land and over sea were explored. The dual view offered by the ATSR-2 aboard ERS-2 was used by Veefkind et al., 1998. The retrieved AOD (aerosol optical depth) values compare favourably with collocated sun photometer measurements, with an accuracy of 0.06 +/- 0.05 in AOD. An algorithm developed for GOME on ERS-2 takes advantage of the low surface reflection in the UV (Veefkind et al., 2000). AOD values retrieved from ATSR-2 and GOME data over western Europe are consistent. The results were used to produce a map of mean AOD values over Europe for one month (Robles-Gonzalez et al., 2000). The ATSR-2 is al- gorithm is now extended with other aerosol types with the aim to apply it over the In- dian Ocean. A new algorithm is being developed for the Ozone Monitoring Instrument (OMI) to be launched in 2003 on the NASA EOS-AURA satellite. It is expected that, based on the different scattering and absorption properties of various aerosol types, five major aerosol classes can be distinguished. The experience with the retrieval of aerosol properties by using several wavelength bands is used to develop an algorithm for Sciamachy to retrieve aerosol properties both over land and over the ocean which takes advantage of the wavelengths from the UV to the IR. The variation of the AOD with wavelength is described by the Angstrom parameter. The AOD and the Angstrom parameter together yield information on the aerosol size distribution, integrated over the column. Analysis of sunphotometer data indicates a relation between the Angstrom parameter and the mass ratio of certain aerosols (black carbon, organic carbon and sea salt) to the total particulate matter. This relation has been further explored and was applied to satellite data over land to

  9. TOFIR: A Tool of Facilitating Information Retrieval - Introduce a Visual Retrieval Model.

    ERIC Educational Resources Information Center

    Zhang, Jin

    2001-01-01

    Introduces a new method for the visualization of information retrieval called TOFIR (Tool of Facilitating Information Retrieval). Discusses the use of angle attributes of a document to construct the angle-based visual space; two-dimensional and three-dimensional visual tools; ambiguity; and future research directions. (Author/LRW)

  10. Improved quantification of HIV-1-infected CD4+ T cells using an optimised method of intracellular HIV-1 gag p24 antigen detection.

    PubMed

    Yang, Hongbing; Yorke, Elisabeth; Hancock, Gemma; Clutton, Genevieve; Sande, Nellia; Angus, Brian; Smyth, Redmond; Mak, Johnson; Dorrell, Lucy

    2013-05-31

    The capacity of CD8+ T cells to inhibit HIV-1 replication in vitro strongly correlates with virus control in vivo. Post-hoc evaluations of HIV-1 vaccine candidates suggest that this immunological parameter is a promising benchmark of vaccine efficacy. Large-scale analysis of CD8+ T cell antiviral activity requires a rapid, robust and economical assay for accurate quantification of HIV-1 infection in primary CD4+ T cells. Detection of intracellular HIV-1 p24 antigen (p24 Ag) by flow cytometry is one such method but it is thought to be less sensitive and quantitative than p24 Ag ELISA. We report that fixation and permeabilisation of HIV-infected cells using paraformaldehyde/50% methanol/Nonidet P-40 instead of a conventional paraformaldehyde/saponin-based protocol improved their detection across multiplicities of infection (MOI) ranging from 10(-2) to 8×10(-5), and by nearly two-fold (p<0.001) at the optimal MOI tested (10(-2)). The frequency of infected cells was strongly correlated with p24 Ag release during culture, thus validating its use as a measure of productive infection. We were also able to quantify infection with a panel of HIV-1 isolates representing the major clades. The protocol described here is rapid and cost-effective compared with ELISA and thus could be a useful component of immune monitoring of HIV-1 vaccines and interventions to reduce viral reservoirs.

  11. Undersampled Phase Retrieval with Outliers

    PubMed Central

    Weller, Daniel S.; Pnueli, Ayelet; Divon, Gilad; Radzyner, Ori; Eldar, Yonina C.; Fessler, Jeffrey A.

    2015-01-01

    This paper proposes a general framework for reconstructing sparse images from undersampled (squared)-magnitude data corrupted with outliers and noise. This phase retrieval method uses a layered approach, combining repeated minimization of a convex majorizer (surrogate for a nonconvex objective function), and iterative optimization of that majorizer using a preconditioned variant of the alternating direction method of multipliers (ADMM). Since phase retrieval is nonconvex, this implementation uses multiple initial majorization vectors. The introduction of a robust 1-norm data fit term that is better adapted to outliers exploits the generality of this framework. The derivation also describes a normalization scheme for the regularization parameter and a known adaptive heuristic for the ADMM penalty parameter. Both 1D Monte Carlo tests and 2D image reconstruction simulations suggest the proposed framework, with the robust data fit term, reduces the reconstruction error for data corrupted with both outliers and additive noise, relative to competing algorithms having the same total computation. PMID:26770999

  12. Topic Models in Information Retrieval

    DTIC Science & Technology

    2007-08-01

    the combination: Hidden Markov Model. Miller et al. (1999) presented a Hidden Markov Model ( HMM ) Information Retrieval system. They take the...combination with different methods to estimate the combination weights. The parameter estimation in the HMM framework is simplified with strict...applied a Hidden Markov Model ( HMM ) framework; Zhai and Lafferty (2002) and Cao et al. (2005) used Expectation Maximization (EM) on the mixture

  13. Gunther Tulip Retrievable Inferior Vena Caval Filters: Indications, Efficacy, Retrieval, and Complications

    SciTech Connect

    Looby, S.; Given, M.F.; Geoghegan, T.; McErlean, A.; Lee, M.J.

    2007-02-15

    Purpose. We evaluated the Gunther Tulip (GT) retrievable inferior vena cava (IVC) filter with regard to indications, filtration efficacy, complications, retrieval window, and use of anticoagulation. Method. A retrospective study was performed of 147 patients (64 men, 83 women; mean age 58.8 years) who underwent retrievable GT filter insertion between 2001 and 2005. The indications for placement included a diagnosis of pulmonary embolism or deep venous thrombosis with a contraindication to anticoagulation (n = 68), pulmonary embolism or deep venous thrombosis while on anticoagulation (n = 49), prophylactic filter placement for high-risk surgical patients with a past history of pulmonary embolism or deep venous thrombosis (n = 20), and a high risk of pulmonary embolism or deep venous thrombosis (n = 10). Forty-nine of the 147 patients did not receive anticoagulation (33.7%) while 96 of 147 patients did, 82 of these receiving warfarin (56.5%), 11 receiving low-molecular weight heparins (7.58%), and 3 receiving antiplatelet agents alone (2.06%). Results. Filter placement was successful in 147 patients (100%). Two patients had two filters inserted. Of the 147 patients, filter deployment was on a permanent basis in 102 and with an intention to retrieve in 45 patients. There were 36 (80%) successful retrievals and 9 (20%) failed retrievals. The mean time to retrieval was 33.6 days. The reasons for failed retrieval included filter struts tightly adherent to the IVC wall (5/9), extreme filter tilt (2/9), and extensive filter thrombus (2/9). Complications included pneumothorax (n = 4), failure of filter expansion (n = 1), and breakthrough pulmonary embolism (n = 1). No IVC thrombotic episodes were recorded. Discussion. The Gunther Tulip retrievable filter can be used as a permanent or a retrievable filter. It is safe and efficacious. GT filters can be safely retrieved at a mean time interval of 33.6 days. The newly developed Celect filter may extend the retrieval interval.

  14. Exploiting salient semantic analysis for information retrieval

    NASA Astrophysics Data System (ADS)

    Luo, Jing; Meng, Bo; Quan, Changqin; Tu, Xinhui

    2016-11-01

    Recently, many Wikipedia-based methods have been proposed to improve the performance of different natural language processing (NLP) tasks, such as semantic relatedness computation, text classification and information retrieval. Among these methods, salient semantic analysis (SSA) has been proven to be an effective way to generate conceptual representation for words or documents. However, its feasibility and effectiveness in information retrieval is mostly unknown. In this paper, we study how to efficiently use SSA to improve the information retrieval performance, and propose a SSA-based retrieval method under the language model framework. First, SSA model is adopted to build conceptual representations for documents and queries. Then, these conceptual representations and the bag-of-words (BOW) representations can be used in combination to estimate the language models of queries and documents. The proposed method is evaluated on several standard text retrieval conference (TREC) collections. Experiment results on standard TREC collections show the proposed models consistently outperform the existing Wikipedia-based retrieval methods.

  15. Sensitivity Analysis for Atmospheric Infrared Sounder (AIRS) CO2 Retrieval

    NASA Technical Reports Server (NTRS)

    Gat, Ilana

    2012-01-01

    The Atmospheric Infrared Sounder (AIRS) is a thermal infrared sensor able to retrieve the daily atmospheric state globally for clear as well as partially cloudy field-of-views. The AIRS spectrometer has 2378 channels sensing from 15.4 micrometers to 3.7 micrometers, of which a small subset in the 15 micrometers region has been selected, to date, for CO2 retrieval. To improve upon the current retrieval method, we extended the retrieval calculations to include a prior estimate component and developed a channel ranking system to optimize the channels and number of channels used. The channel ranking system uses a mathematical formalism to rapidly process and assess the retrieval potential of large numbers of channels. Implementing this system, we identifed a larger optimized subset of AIRS channels that can decrease retrieval errors and minimize the overall sensitivity to other iridescent contributors, such as water vapor, ozone, and atmospheric temperature. This methodology selects channels globally by accounting for the latitudinal, longitudinal, and seasonal dependencies of the subset. The new methodology increases accuracy in AIRS CO2 as well as other retrievals and enables the extension of retrieved CO2 vertical profiles to altitudes ranging from the lower troposphere to upper stratosphere. The extended retrieval method for CO2 vertical profile estimation using a maximum-likelihood estimation method. We use model data to demonstrate the beneficial impact of the extended retrieval method using the new channel ranking system on CO2 retrieval.

  16. Quantitative evaluation of a single-distance phase-retrieval method applied on in-line phase-contrast images of a mouse lung.

    PubMed

    Mohammadi, Sara; Larsson, Emanuel; Alves, Frauke; Dal Monego, Simeone; Biffi, Stefania; Garrovo, Chiara; Lorenzon, Andrea; Tromba, Giuliana; Dullin, Christian

    2014-07-01

    Propagation-based X-ray phase-contrast computed tomography (PBI) has already proven its potential in a great variety of soft-tissue-related applications including lung imaging. However, the strong edge enhancement, caused by the phase effects, often hampers image segmentation and therefore the quantitative analysis of data sets. Here, the benefits of applying single-distance phase retrieval prior to the three-dimensional reconstruction (PhR) are discussed and quantified compared with three-dimensional reconstructions of conventional PBI data sets in terms of contrast-to-noise ratio (CNR) and preservation of image features. The PhR data sets show more than a tenfold higher CNR and only minor blurring of the edges when compared with PBI in a predominately absorption-based set-up. Accordingly, phase retrieval increases the sensitivity and provides more functionality in computed tomography imaging.

  17. Quantitative evaluation of a single-distance phase-retrieval method applied on in-line phase-contrast images of a mouse lung

    PubMed Central

    Mohammadi, Sara; Larsson, Emanuel; Alves, Frauke; Dal Monego, Simeone; Biffi, Stefania; Garrovo, Chiara; Lorenzon, Andrea; Tromba, Giuliana; Dullin, Christian

    2014-01-01

    Propagation-based X-ray phase-contrast computed tomography (PBI) has already proven its potential in a great variety of soft-tissue-related applications including lung imaging. However, the strong edge enhancement, caused by the phase effects, often hampers image segmentation and therefore the quantitative analysis of data sets. Here, the benefits of applying single-distance phase retrieval prior to the three-dimensional reconstruction (PhR) are discussed and quantified compared with three-dimensional reconstructions of conventional PBI data sets in terms of contrast-to-noise ratio (CNR) and preservation of image features. The PhR data sets show more than a tenfold higher CNR and only minor blurring of the edges when compared with PBI in a predominately absorption-based set-up. Accordingly, phase retrieval increases the sensitivity and provides more functionality in computed tomography imaging. PMID:24971975

  18. Wire Retrieves Broken Pin

    NASA Technical Reports Server (NTRS)

    Burow, G. H.

    1984-01-01

    Safety wire retains pieces of broken tool. Retrieval wire running through shaft of tool used to pull pieces of tool out of hole, should tool break during use. Safety wire concept suitable for pins subject to deflection or breakage.

  19. Data-Retrieval System

    NASA Technical Reports Server (NTRS)

    Lawrence, P. R.

    1982-01-01

    NASA Graphics and Display System (NGDS) helps solve problem of data retrieval for management. Since NGDS is interactive online system, user controls display content and format, and has capability of always working with latest version of his data.

  20. Information retrieval system

    NASA Technical Reports Server (NTRS)

    Berg, R. F.; Holcomb, J. E.; Kelroy, E. A.; Levine, D. A.; Mee, C., III

    1970-01-01

    Generalized information storage and retrieval system capable of generating and maintaining a file, gathering statistics, sorting output, and generating final reports for output is reviewed. File generation and file maintenance programs written for the system are general purpose routines.

  1. Retrieving Patent Information Online

    ERIC Educational Resources Information Center

    Kaback, Stuart M.

    1978-01-01

    This paper discusses patent information retrieval from online files in terms of types of questions, file contents, coverage, timeliness, and other file variations. CLAIMS, Derwent, WPI, APIPAT and Chemical Abstracts Service are described. (KP)

  2. Content-Based Medical Image Retrieval

    NASA Astrophysics Data System (ADS)

    Müller, Henning; Deserno, Thomas M.

    This chapter details the necessity for alternative access concepts to the currently mainly text-based methods in medical information retrieval. This need is partly due to the large amount of visual data produced, the increasing variety of medical imaging data and changing user patterns. The stored visual data contain large amounts of unused information that, if well exploited, can help diagnosis, teaching and research. The chapter briefly reviews the history of image retrieval and its general methods before technologies that have been developed in the medical domain are focussed. We also discuss evaluation of medical content-based image retrieval (CBIR) systems and conclude with pointing out their strengths, gaps, and further developments. As examples, the MedGIFT project and the Image Retrieval in Medical Applications (IRMA) framework are presented.

  3. Diverse Endogenous Antigens for Mouse Natural Killer T Cells: Self-Antigens That Are Not Glycosphingolipids

    PubMed Central

    Pei, Bo; Speak, Anneliese O; Shepherd, Dawn; Butters, Terry; Cerundolo, Vincenzo; Platt, Frances M; Kronenberg, Mitchell

    2011-01-01

    Natural killer T cells with an invariant antigen receptor (iNKT cells) represent a highly conserved and unique subset of T lymphocytes having properties of innate and adaptive immune cells. They have been reported to regulate a variety of immune responses, including the response to cancers and the development of autoimmunity. The development and activation of iNKT cells is dependent on self-antigens presented by the CD1d antigen-presenting molecule. It is widely believed that these self-antigens are glycosphingolipids (GSLs), molecules that contain ceramide as the lipid backbone. Here we used a variety of methods to show that mammalian antigens for mouse iNKT cells need not be GSLs, including the use of cell lines deficient in GSL biosynthesis and an inhibitor of GSL biosynthesis. Presentation of these antigens required the expression of CD1d molecules that could traffic to late endosomes, the site where self-antigen is acquired. Extracts of antigen-presenting cells (APCs) contain a self-antigen that could stimulate iNKT cells when added to plates coated with soluble, recombinant CD1d molecules. The antigen(s) in these extracts are resistant to sphingolipid-specific hydrolase digestion, consistent with the results using live APCs. Lyosphosphatidylcholine, a potential self-antigen that activated human iNKT cell lines, did not activate mouse iNKT cell hybridomas. Our data indicate that there may be more than one type of self-antigen for iNKT cells, that the self-antigens comparing mouse and human may not be conserved, and that the search to identify these molecules should not be confined to GSLs. PMID:21191069

  4. A Bayesian approach to microwave precipitation profile retrieval

    NASA Technical Reports Server (NTRS)

    Evans, K. Franklin; Turk, Joseph; Wong, Takmeng; Stephens, Graeme L.

    1995-01-01

    A multichannel passive microwave precipitation retrieval algorithm is developed. Bayes theorem is used to combine statistical information from numerical cloud models with forward radiative transfer modeling. A multivariate lognormal prior probability distribution contains the covariance information about hydrometeor distribution that resolves the nonuniqueness inherent in the inversion process. Hydrometeor profiles are retrieved by maximizing the posterior probability density for each vector of observations. The hydrometeor profile retrieval method is tested with data from the Advanced Microwave Precipitation Radiometer (10, 19, 37, and 85 GHz) of convection over ocean and land in Florida. The CP-2 multiparameter radar data are used to verify the retrieved profiles. The results show that the method can retrieve approximate hydrometeor profiles, with larger errors over land than water. There is considerably greater accuracy in the retrieval of integrated hydrometeor contents than of profiles. Many of the retrieval errors are traced to problems with the cloud model microphysical information, and future improvements to the algorithm are suggested.

  5. Retrieval of constituent mixing ratios from limb thermal emission spectra

    NASA Technical Reports Server (NTRS)

    Shaffer, William A.; Kunde, Virgil G.; Conrath, Barney J.

    1988-01-01

    An onion-peeling iterative, least-squares relaxation method to retrieve mixing ratio profiles from limb thermal emission spectra is presented. The method has been tested on synthetic data, containing various amounts of added random noise for O3, HNO3, and N2O. The retrieval method is used to obtain O3 and HNO3 mixing ratio profiles from high-resolution thermal emission spectra. Results of the retrievals compare favorably with those obtained previously.

  6. A Ground Flash Fraction Retrieval Algorithm for GLM

    NASA Technical Reports Server (NTRS)

    Koshak, William J.

    2010-01-01

    A Bayesian inversion method is introduced for retrieving the fraction of ground flashes in a set of N lightning observed by a satellite lightning imager (such as the Geostationary Lightning Mapper, GLM). An exponential model is applied as a physically reasonable constraint to describe the measured lightning optical parameter distributions. Population statistics (i.e., the mean and variance) are invoked to add additional constraints to the retrieval process. The Maximum A Posteriori (MAP) solution is employed. The approach is tested by performing simulated retrievals, and retrieval error statistics are provided. The approach is feasible for N greater than 2000, and retrieval errors decrease as N is increased.

  7. Neural systems behind word and concept retrieval.

    PubMed

    Damasio, H; Tranel, D; Grabowski, T; Adolphs, R; Damasio, A

    2004-01-01

    Using both the lesion method and functional imaging (positron emission tomography) in large cohorts of subjects investigated with the same experimental tasks, we tested the following hypotheses: (A) that the retrieval of words which denote concrete entities belonging to distinct conceptual categories depends upon partially segregated regions in higher-order cortices of the left temporal lobe; and (B) that the retrieval of conceptual knowledge pertaining to the same concrete entities also depends on partially segregated regions; however, those regions will be different from those postulated in hypothesis A, and located predominantly in the right hemisphere (the second hypothesis tested only with the lesion method). The analyses provide support for hypothesis A in that several regions outside the classical Broca and Wernicke language areas are involved in name retrieval of concrete entities, and that there is a partial segregation in the temporal lobe with respect to the conceptual category to which the entities belong, and partial support for hypothesis B in that retrieval of conceptual knowledge is partially segregated from name retrieval in the lesion study. Those regions identified here are seen as parts of flexible, multi-component systems serving concept and word retrieval for concrete entities belonging to different conceptual categories. By comparing different approaches the article also addresses a number of method issues that have surfaced in recent studies in this field.

  8. Phase retrieval from single biomolecule diffraction pattern.

    PubMed

    Ikeda, Shiro; Kono, Hidetoshi

    2012-02-13

    In this paper, we propose the SPR (sparse phase retrieval) method, which is a new phase retrieval method for coherent x-ray diffraction imaging (CXDI). Conventional phase retrieval methods effectively solve the problem for high signal-to-noise ratio measurements, but would not be sufficient for single biomolecular imaging which is expected to be realized with femto-second x-ray free electron laser pulses. The SPR method is based on the Bayesian statistics. It does not need to set the object boundary constraint that is required by the commonly used hybrid input-output (HIO) method, instead a prior distribution is defined with an exponential distribution and used for the estimation. Simulation results demonstrate that the proposed method reconstructs the electron density under a noisy condition even some central pixels are masked.

  9. Cellular Antigens in the Structure of Venezuelan Equine Encephalomyelitis Virus

    DTIC Science & Technology

    1975-02-28

    the previously described method. 𔄁xzracts of the Dolichos biflorus L seeds (to calculate antigen A) and Cytisus sessilifolius L. seeds (to calculate...find the group antigen A and phytohemagglutinins "from seeds Cytisus sessilefolius L. in order to calculate antigen H. As h -6- "Fable 4 shows virus

  10. A computational framework for influenza antigenic cartography.

    PubMed

    Cai, Zhipeng; Zhang, Tong; Wan, Xiu-Feng

    2010-10-07

    Influenza viruses have been responsible for large losses of lives around the world and continue to present a great public health challenge. Antigenic characterization based on hemagglutination inhibition (HI) assay is one of the routine procedures for influenza vaccine strain selection. However, HI assay is only a crude experiment reflecting the antigenic correlations among testing antigens (viruses) and reference antisera (antibodies). Moreover, antigenic characterization is usually based on more than one HI dataset. The combination of multiple datasets results in an incomplete HI matrix with many unobserved entries. This paper proposes a new computational framework for constructing an influenza antigenic cartography from this incomplete matrix, which we refer to as Matrix Completion-Multidimensional Scaling (MC-MDS). In this approach, we first reconstruct the HI matrices with viruses and antibodies using low-rank matrix completion, and then generate the two-dimensional antigenic cartography using multidimensional scaling. Moreover, for influenza HI tables with herd immunity effect (such as those from Human influenza viruses), we propose a temporal model to reduce the inherent temporal bias of HI tables caused by herd immunity. By applying our method in HI datasets containing H3N2 influenza A viruses isolated from 1968 to 2003, we identified eleven clusters of antigenic variants, representing all major antigenic drift events in these 36 years. Our results showed that both the completed HI matrix and the antigenic cartography obtained via MC-MDS are useful in identifying influenza antigenic variants and thus can be used to facilitate influenza vaccine strain selection. The webserver is available at http://sysbio.cvm.msstate.edu/AntigenMap.

  11. Multispectral vegetative canopy parameter retrieval

    NASA Astrophysics Data System (ADS)

    Borel, Christoph C.; Bunker, David J.

    2011-11-01

    Precision agriculture, forestry and environmental remote sensing are applications uniquely suited to the 8 bands that DigitalGlobe's WorldView-2 provides. At the fine spatial resolution of 0.5 m (panchromatic) and 2 m (multispectral) individual trees can be readily resolved. Recent research [1] has shown that it is possible for hyper-spectral data to invert plant reflectance spectra and estimate nitrogen content, leaf water content, leaf structure, canopy leaf area index and, for sparse canopies, also soil reflectance. The retrieval is based on inverting the SAIL (Scattering by Arbitrary Inclined Leaves) vegetation radiative transfer model for the canopy structure and the reflectance model PROSPECT4/5 for the leaf reflectance. Working on the paper [1] confirmed that a limited number of adjacent bands covering just the visible and near infrared can retrieve the parameters as well, opening up the possibility that this method can be used to analyze multi-spectral WV-2 data. Thus it seems possible to create WV-2 specific inversions using 8 bands and apply them to imagery of various vegetation covered surfaces of agricultural and environmental interest. The capability of retrieving leaf water content and nitrogen content has important applications in determining the health of vegetation, e.g. plant growth status, disease mapping, quantitative drought assessment, nitrogen deficiency, plant vigor, yield, etc.

  12. [Use in immunohistochemical studies of a method of restoration of antigenic specificity as affected by microwaves in tissue fixed with formalin and embedded in paraffin].

    PubMed

    Gurevich, L E; Isakov, V A

    1999-01-01

    Recovery of specific antigenic characteristics using microwave treatment of paraffin sections of tissues fixed by formalin allows to extend spectrum of antibodies for immunohistochemical diagnosis. Microwaves enable the reaction on the material prepared according to the standard technique, and macropreparations long stored in formalin and archive blocks.

  13. Retrieval Studies with LIDORT

    NASA Technical Reports Server (NTRS)

    Bhartia, P. K. (Technical Monitor); Spurr, Robert J. D.; Chance, K. V.

    2003-01-01

    This short program of LIDORT-based research in atmospheric trace gas retrieval was conducted over the 1 year period 01 July 2002 to 30 June 2003. After consultation with the NASA reporting officer, the first of the two original proposal activities (development of a direct-fitting total O3 column retrieval algorithm with operational capability for GOME data) was replaced by other tasks. The three activities addressed were: (1) Sensitivity studies for column and profile retrieval of NO2 distributions from a new generation of multi-axis ground-based spectrometers; (2) use of the LIDORT-RRS model to determine the effect of inelastic rotational Raman scattering at SBUV wavelengths; (3) an examination of ozone profile weighting functions in the presence of optically thick tropospheric clouds.

  14. Retrieval of transuranic waste

    SciTech Connect

    Not Available

    1988-07-19

    Soil removal and drum retrieval equipment are being developed and demonstrated in support of the Transuranic Waste Facility (TWF) program. Accomplishments to date include: Soil removal equipment, a telescoping excavator and high velocity vacuum truck were selected for soil removal; Preliminary demonstrations, the capabilities of both the telescoping excavator and vacuum truck were demonstrated by the vendors; Shielding Lifting Canister, Functional parameters were defined and design is 95% complete; and Full-scale demonstration, SRL test mounds were selected for a full-scale drum retrieval demonstration. Further development will include an integrated full-scale demonstration. Several drums buried in non-radioactive TRU test mounds will be retrieved. Planning for the demonstration is underway. A telescoping excavator is being acquired, the shielded lifting canister will be fabricated and pre-tested, and a vacuum truck will be leased. 5 figs.

  15. Human factors analysis and design methods for nuclear waste retrieval systems. Volume III. User's guide for the computerized event-tree analysis technique. [CETAT computer program

    SciTech Connect

    Casey, S.M.; Deretsky, Z.

    1980-08-01

    This document provides detailed instructions for using the Computerized Event-Tree Analysis Technique (CETAT), a program designed to assist a human factors analyst in predicting event probabilities in complex man-machine configurations found in waste retrieval systems. The instructions contained herein describe how to (a) identify the scope of a CETAT analysis, (b) develop operator performance data, (c) enter an event-tree structure, (d) modify a data base, and (e) analyze event paths and man-machine system configurations. Designed to serve as a tool for developing, organizing, and analyzing operator-initiated event probabilities, CETAT simplifies the tasks of the experienced systems analyst by organizing large amounts of data and performing cumbersome and time consuming arithmetic calculations. The principal uses of CETAT in the waste retrieval development project will be to develop models of system reliability and evaluate alternative equipment designs and operator tasks. As with any automated technique, however, the value of the output will be a function of the knowledge and skill of the analyst using the program.

  16. Understanding Spatial Variability and Point Classification Implications on Methods for Retrieval of Leaf Orientation for Effective Leaf Area Index from Terrestrial Laser Scanning.

    NASA Astrophysics Data System (ADS)

    Richardson, J. J.; Moskal, L. M.; Zheng, G.; Kato, A.

    2015-12-01

    Tree leaf orientation, including the distribution of the inclinational and azimuthal angles in the canopy, is an important attribute of forest canopy architecture and is critical in determining the within and below canopy solar radiation regimes. We demonstrate techniques for indirectly and nondestructively retrieves foliage elements' orientation and distribution from point cloud data (PCD) obtained using a terrestrial laser scanning (TLS) approach.An equation with a single parameter for characterizing the leaf angular distribution of crowns was developed. The TLS-based algorithm captures 97.4% (RMSE =1 .094 degrees, p<0.001) variation of the leaf inclination angle compared to manual measurements for an artificial tree. When applied to a live tree seedling and a mature tree crown, the TLS-based algorithm predicts 78.51% (RMSE =1 .225 degrees, p<0.001) and 57.28% (RMSE =4 .412 degrees, p<0.001) of the angular variability, respectively. Furthermore we demonstrate our approach for retrieve of biophysical characteristics of the forest canopy including extinction coefficient, gap fraction, overlapping effect, and effective leaf area Index (ELAI). Out ELAI model captures 88.7% (rmse =0 .007, p<0.001, andn = 30) variation of the destructive-sample-based leaf area measurement results and 89.1% (rmse =0 .01; p<0.001) of the variation in results from digital hemispherical photographs. Finally we demonstrate how scanner setup which includes lateral scans can reduce effects of occlusion in terrestrial laser data collection.

  17. New methods for the retrieval of chlorophyll red fluorescence from hyperspectral satellite instruments: simulations and application to GOME-2 and SCIAMACHY

    NASA Astrophysics Data System (ADS)

    Joiner, Joanna; Yoshida, Yasuko; Guanter, Luis; Middleton, Elizabeth M.

    2016-08-01

    Global satellite measurements of solar-induced fluorescence (SIF) from chlorophyll over land and ocean have proven useful for a number of different applications related to physiology, phenology, and productivity of plants and phytoplankton. Terrestrial chlorophyll fluorescence is emitted throughout the red and far-red spectrum, producing two broad peaks near 683 and 736 nm. From ocean surfaces, phytoplankton fluorescence emissions are entirely from the red region (683 nm peak). Studies using satellite-derived SIF over land have focused almost exclusively on measurements in the far red (wavelengths > 712 nm), since those are the most easily obtained with existing instrumentation. Here, we examine new ways to use existing hyperspectral satellite data sets to retrieve red SIF (wavelengths < 712 nm) over both land and ocean. Red SIF is thought to provide complementary information to that from the far red for terrestrial vegetation. The satellite instruments that we use were designed to make atmospheric trace-gas measurements and are therefore not optimal for observing SIF; they have coarse spatial resolution and only moderate spectral resolution (0.5 nm). Nevertheless, these instruments, the Global Ozone Monitoring Instrument 2 (GOME-2) and the SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY), offer a unique opportunity to compare red and far-red terrestrial SIF at regional spatial scales. Terrestrial SIF has been estimated with ground-, aircraft-, or satellite-based instruments by measuring the filling-in of atmospheric and/or solar absorption spectral features by SIF. Our approach makes use of the oxygen (O2) γ band that is not affected by SIF. The SIF-free O2 γ band helps to estimate absorption within the spectrally variable O2 B band, which is filled in by red SIF. SIF also fills in the spectrally stable solar Fraunhofer lines (SFLs) at wavelengths both inside and just outside the O2 B band, which further helps to estimate red SIF

  18. Transcutaneous antigen delivery system

    PubMed Central

    Lee, Mi-Young; Shin, Meong-Cheol; Yang, Victor C.

    2013-01-01

    Transcutaneous immunization refers to the topical application of antigens onto the epidermis. Transcutaneous immunization targeting the Langerhans cells of the skin has received much attention due to its safe, needle-free, and noninvasive antigen delivery. The skin has important immunological functions with unique roles for antigen-presenting cells such as epidermal Langerhans cells and dermal dendritic cells. In recent years, novel vaccine delivery strategies have continually been developed; however, transcutaneous immunization has not yet been fully exploited due to the penetration barrier represented by the stratum corneum, which inhibits the transport of antigens and adjuvants. Herein we review recent achievements in transcutaneous immunization, focusing on the various strategies for the enhancement of antigen delivery and vaccination efficacy. [BMB Reports 2013; 46(1): 17-24] PMID:23351379

  19. Microscale purification of antigen-specific antibodies.

    PubMed

    Brown, Eric P; Normandin, Erica; Osei-Owusu, Nana Yaw; Mahan, Alison E; Chan, Ying N; Lai, Jennifer I; Vaccari, Monica; Rao, Mangala; Franchini, Genoveffa; Alter, Galit; Ackerman, Margaret E

    2015-10-01

    Glycosylation of the Fc domain is an important driver of antibody effector function. While assessment of antibody glycoform compositions observed across total plasma IgG has identified differences associated with a variety of clinical conditions, in many cases it is the glycosylation state of only antibodies against a specific antigen or set of antigens that may be of interest, for example, in defining the potential effector function of antibodies produced during disease or after vaccination. Historically, glycoprofiling such antigen-specific antibodies in clinical samples has been challenging due to their low prevalence, the high sample requirement for most methods of glycan determination, and the lack of high-throughput purification methods. New methods of glycoprofiling with lower sample requirements and higher throughput have motivated the development of microscale and automatable methods for purification of antigen-specific antibodies from polyclonal sources such as clinical serum samples. In this work, we present a robot-compatible 96-well plate-based method for purification of antigen-specific antibodies, suitable for such population level glycosylation screening. We demonstrate the utility of this method across multiple antibody sources, using both purified plasma IgG and plasma, and across multiple different antigen types, with enrichment factors greater than 1000-fold observed. Using an on-column IdeS protease treatment, we further describe staged release of Fc and Fab domains, allowing for glycoprofiling of each domain.

  20. Microscale purification of antigen-specific antibodies

    PubMed Central

    Brown, Eric P.; Normandin, Erica; Osei-Owusu, Nana Yaw; Mahan, Alison E.; Chan, Ying N.; Lai, Jennifer I.; Vaccari, Monica; Rao, Mangala; Franchini, Genoveffa; Alter, Galit; Ackerman, Margaret E.

    2015-01-01

    Glycosylation of the Fc domain is an important driver of antibody effector function. While assessment of antibody glycoform compositions observed across total plasma IgG has identified differences associated with a variety of clinical conditions, in many cases it is the glycosylation state of only antibodies against a specific antigen or set of antigens that may be of interest, for example, in defining the potential effector function of antibodies produced during disease or after vaccination. Historically, glycoprofiling such antigen-specific antibodies in clinical samples has been challenging due to their low prevalence, the high sample requirement for most methods of glycan determination, and the lack of high-throughput purification methods. New methods of glycoprofiling with lower sample requirements and higher throughput have motivated the development of microscale and automatable methods for purification of antigen-specific antibodies from polyclonal sources such as clinical serum samples. In this work, we present a robot-compatible 96-well plate-based method for purification of antigen-specific antibodies, suitable for such population level glycosylation screening. We demonstrate the utility of this method across multiple antibody sources, using both purified plasma IgG and plasma, and across multiple different antigen types, with enrichment factors greater than 1000-fold observed. Using an on-column IdeS protease treatment, we further describe staged release of Fc and Fab domains, allowing for glycoprofiling of each domain. PMID:26078040

  1. Isolation and In vivo Transfer of Antigen Presenting Cells

    PubMed Central

    Arora, Pooja; Kharkwal, Shalu Sharma; Porcelli, Steven A.

    2016-01-01

    Transfer of antigen presenting cells in vivo is a method used by immunologists to examine the potency of antigen presentation by a selected population of cells. This method is most commonly used to analyze presentation of protein antigens to MHC class I or II restricted T cells, but it can also be used for studies of nonconventional antigens such as CD1-presented lipids. In a recent study focusing on CD1d-restricted glycolipid antigen presentation to Natural Killer T cells, we compared antigen presenting properties of splenic B cells, CD8αPos dendritc cells (DCs) and CD8αNeg DCs (Arora et al., 2014). This protocol describes the detailed method used for isolation of these cell populations, and their transfer into recipient mice to analyze their antigen presenting properties. PMID:27390759

  2. Retrieval Hierarchies in Hypertext.

    ERIC Educational Resources Information Center

    Rada, Roy; And Others

    1993-01-01

    Describes a collaborative, reuse hypertext system, called MUCH (Many Using and Creating Hypertext). The novel browsing and retrieval characteristics of the system are based on a semantic net connecting documents, thesauri, and discussions. Word patterns are documented for reuse, creating and reorganizing network traversal according to user…

  3. Information Retrieval by Television.

    ERIC Educational Resources Information Center

    Billowes, C. A.

    1968-01-01

    Bell Canada, the Public School and Collegiate Institute Boards of Ottawa, and the Ontario Institute for Studies in Education are collaborating on an educational television project which will provide a retrieval system that can supply any given program at any time under the control of the classroom teacher. Four schools in Ottawa will participate…

  4. Collaborative Information Retrieval.

    ERIC Educational Resources Information Center

    Bruce, Harry; Fidel, Raya

    1999-01-01

    Researchers from the University of Washington, Microsoft Research, Boeing, and Risoe National Laboratory in Denmark have embarked on a project to explore the manifestations of Collaborative Information Retrieval (CIR) in work settings and to propose technological innovations and organizational changes that can support, facilitate, and improve CIR.…

  5. Feedback in Information Retrieval.

    ERIC Educational Resources Information Center

    Spink, Amanda; Losee, Robert M.

    1996-01-01

    As Information Retrieval (IR) has evolved, it has become a highly interactive process, rooted in cognitive and situational contexts. Consequently the traditional cybernetic-based IR model does not suffice for interactive IR or the human approach to IR. Reviews different views of feedback in IR and their relationship to cybernetic and social…

  6. Information Retrieval in Physics.

    ERIC Educational Resources Information Center

    Herschman, Arthur

    Discussed in this paper are the information problems in physics and the current program of the American Institute of Physics (AIP) being conducted in an attempt to develop an information retrieval system. The seriousness of the need is described by means of graphs indicating the exponential rise in the number of physics publications in the last…

  7. Music Information Retrieval.

    ERIC Educational Resources Information Center

    Downie, J. Stephen

    2003-01-01

    Identifies MIR (Music Information Retrieval) computer system problems, historic influences, current state-of-the-art, and future MIR solutions through an examination of the multidisciplinary approach to MIR. Highlights include pitch; temporal factors; harmonics; tone; editorial, textual, and bibliographic facets; multicultural factors; locating…

  8. Evaluation of Retrievability of the Gunther Tulip Vena Cava Filter

    SciTech Connect

    Yamagami, Takuji Kato, Takeharu; Hirota, Tatsuya; Yoshimatsu, Rika; Matsumoto, Tomohiro; Nishimura, Tsunehiko

    2007-04-15

    Purpose. To evaluate the feasibility and safety of withdrawal of a Gunther tulip retrievable vena cava filter (GTF). Methods. Between June 2001 and December 2005, at our institution 86 GTFs were implanted for temporary caval filtration in 59 patients (37 women, 22 men; mean age 59.3 years, range 18-87 years). For GTFs retrieved thereafter, we retrospectively reviewed the following parameters: rate of success in retrieval, degree of trapped thrombus in the filter, and complications during retrieval. Results. Worsening of or new development of pulmonary embolism after filter implantation did not occur in any patient. Of the 86 GTFs implanted, retrieval of 80 was attempted. Among those 80 filters, 77 (96%) were successfully retrieved (with the standard method, n = 72; with the modified method, n = 5) without any complication. The period of implantation of the retrieved filters was 13.4 {+-} 4.2 days. In the 5 filters that were filled to a height of {>=} 1/4 with trapped thrombus, retrieval was performed after attempts were made to decrease trapped thrombi. In addition, a temporary filter or another GTF was temporarily placed at the cephalad level of the GTF during this removal procedure. Conclusion. GTFs can be retrieved in the majority of cases. Even when encountering situations in which the filter could not be removed using the standard method, withdrawal was possible in a high frequency of cases through various trials using modified methods.

  9. Retrieval of ash properties from IASI measurements

    NASA Astrophysics Data System (ADS)

    Ventress, Lucy J.; McGarragh, Gregory; Carboni, Elisa; Smith, Andrew J.; Grainger, Roy G.

    2016-11-01

    A new optimal estimation algorithm for the retrieval of volcanic ash properties has been developed for use with the Infrared Atmospheric Sounding Interferometer (IASI). The retrieval method uses the wave number range 680-1200 cm-1, which contains window channels, the CO2 ν2 band (used for the height retrieval), and the O3 ν3 band.Assuming a single infinitely (geometrically) thin ash plume and combining this with the output from the radiative transfer model RTTOV, the retrieval algorithm produces the most probable values for the ash optical depth (AOD), particle effective radius, plume top height, and effective radiating temperature. A comprehensive uncertainty budget is obtained for each pixel. Improvements to the algorithm through the use of different measurement error covariance matrices are explored, comparing the results from a sensitivity study of the retrieval process using covariance matrices trained on either clear-sky or cloudy scenes. The result showed that, due to the smaller variance contained within it, the clear-sky covariance matrix is preferable. However, if the retrieval fails to pass the quality control tests, the cloudy covariance matrix is implemented.The retrieval algorithm is applied to scenes from the Eyjafjallajökull eruption in 2010, and the retrieved parameters are compared to ancillary data sources. The ash optical depth gives a root mean square error (RMSE) difference of 0.46 when compared to retrievals from the MODerate-resolution Imaging Spectroradiometer (MODIS) instrument for all pixels and an improved RMSE of 0.2 for low optical depths (AOD < 0.1). Measurements from the Facility for Airborne Atmospheric Measurements (FAAM) and Deutsches Zentrum für Luft- und Raumfahrt e.V. (DLR) flight campaigns are used to verify the retrieved particle effective radius, with the retrieved distribution of sizes for the scene showing excellent consistency. Further, the plume top altitudes are compared to derived cloud-top altitudes from the Cloud

  10. Compressed sensing for phase retrieval.

    PubMed

    Newton, Marcus C

    2012-05-01

    To date there are several iterative techniques that enjoy moderate success when reconstructing phase information, where only intensity measurements are made. There remains, however, a number of cases in which conventional approaches are unsuccessful. In the last decade, the theory of compressed sensing has emerged and provides a route to solving convex optimisation problems exactly via ℓ(1)-norm minimization. Here the application of compressed sensing to phase retrieval in a nonconvex setting is reported. An algorithm is presented that applies reweighted ℓ(1)-norm minimization to yield accurate reconstruction where conventional methods fail.

  11. Etiologic diagnosis of diarrheal disease of calves: frequency and methods for detecting enterotoxin and K99 antigen production by Escherichia cola.

    PubMed

    Moon, H W; Whipp, S C; Skartvedt, S M

    1976-09-01

    Escherichia coli isolated from calves in Minnesota and Montana were tested for enterotoxigenicity via bio-assay of cell-free broth culture fluid and for K99 antigen via a serum agglutination test. Infant mice were used to assay for heat-stable enterotoxin (ST), and adrenal cells in culture were used to assay for heat-labile enterotoxin (LT). Forty-six of the 345 E coli isolates produced ST enterotoxin, but none produced LT enterotoxin. Thirty-five of the 46 enterotoxigenic isolates had K99 antigen, and only 9 of 66 nonenterotoxigenic isolates so tested had this antigen. The enterotoxigenicity of 28 additional E coli isolates known or suspected to be calf enteropathogens and provided by investigators from 3 different laboratories was also tested. All isolates from 2 laboratories produced ST but not LT. All isolates from the 3rd laboratory produced LT but not ST. Escherichia coli organisms that were positive in the infant mouse assay also caused positive ligated, jejunal-loop responses in calves and in 9-day-old (but not in 5-week-old) pigs. It was concluded that the infant mouse and adrenal cell tests for ST and LT, combined with the agglutination test for K99, would be useful in the diagnosis of enteric enterotoxic colibacillosis of calves.

  12. Phase retrieval by coherent modulation imaging

    SciTech Connect

    Zhang, Fucai; Chen, Bo; Morrison, Graeme R.; Vila-Comamala, Joan; Guizar-Sicairos, Manuel; Robinson, Ian K.

    2016-11-18

    Phase retrieval is a long-standing problem in imaging when only the intensity of the wavefield can be recorded. Coherent diffraction imaging (CDI) is a lensless technique that uses iterative algorithms to recover amplitude and phase contrast images from diffraction intensity data. For general samples, phase retrieval from a single diffraction pattern has been an algorithmic and experimental challenge. Here we report a method of phase retrieval that uses a known modulation of the sample exit-wave. This coherent modulation imaging (CMI) method removes inherent ambiguities of CDI and uses a reliable, rapidly converging iterative algorithm involving three planes. It works for extended samples, does not require tight support for convergence, and relaxes dynamic range requirements on the detector. CMI provides a robust method for imaging in materials and biological science, while its single-shot capability will benefit the investigation of dynamical processes with pulsed sources, such as X-ray free electron laser.

  13. Phase retrieval by coherent modulation imaging.

    PubMed

    Zhang, Fucai; Chen, Bo; Morrison, Graeme R; Vila-Comamala, Joan; Guizar-Sicairos, Manuel; Robinson, Ian K

    2016-11-18

    Phase retrieval is a long-standing problem in imaging when only the intensity of the wavefield can be recorded. Coherent diffraction imaging is a lensless technique that uses iterative algorithms to recover amplitude and phase contrast images from diffraction intensity data. For general samples, phase retrieval from a single-diffraction pattern has been an algorithmic and experimental challenge. Here we report a method of phase retrieval that uses a known modulation of the sample exit wave. This coherent modulation imaging method removes inherent ambiguities of coherent diffraction imaging and uses a reliable, rapidly converging iterative algorithm involving three planes. It works for extended samples, does not require tight support for convergence and relaxes dynamic range requirements on the detector. Coherent modulation imaging provides a robust method for imaging in materials and biological science, while its single-shot capability will benefit the investigation of dynamical processes with pulsed sources, such as X-ray free-electron lasers.

  14. Phase retrieval by coherent modulation imaging

    PubMed Central

    Zhang, Fucai; Chen, Bo; Morrison, Graeme R.; Vila-Comamala, Joan; Guizar-Sicairos, Manuel; Robinson, Ian K.

    2016-01-01

    Phase retrieval is a long-standing problem in imaging when only the intensity of the wavefield can be recorded. Coherent diffraction imaging is a lensless technique that uses iterative algorithms to recover amplitude and phase contrast images from diffraction intensity data. For general samples, phase retrieval from a single-diffraction pattern has been an algorithmic and experimental challenge. Here we report a method of phase retrieval that uses a known modulation of the sample exit wave. This coherent modulation imaging method removes inherent ambiguities of coherent diffraction imaging and uses a reliable, rapidly converging iterative algorithm involving three planes. It works for extended samples, does not require tight support for convergence and relaxes dynamic range requirements on the detector. Coherent modulation imaging provides a robust method for imaging in materials and biological science, while its single-shot capability will benefit the investigation of dynamical processes with pulsed sources, such as X-ray free-electron lasers. PMID:27857061

  15. Phase retrieval by coherent modulation imaging

    NASA Astrophysics Data System (ADS)

    Zhang, Fucai; Chen, Bo; Morrison, Graeme R.; Vila-Comamala, Joan; Guizar-Sicairos, Manuel; Robinson, Ian K.

    2016-11-01

    Phase retrieval is a long-standing problem in imaging when only the intensity of the wavefield can be recorded. Coherent diffraction imaging is a lensless technique that uses iterative algorithms to recover amplitude and phase contrast images from diffraction intensity data. For general samples, phase retrieval from a single-diffraction pattern has been an algorithmic and experimental challenge. Here we report a method of phase retrieval that uses a known modulation of the sample exit wave. This coherent modulation imaging method removes inherent ambiguities of coherent diffraction imaging and uses a reliable, rapidly converging iterative algorithm involving three planes. It works for extended samples, does not require tight support for convergence and relaxes dynamic range requirements on the detector. Coherent modulation imaging provides a robust method for imaging in materials and biological science, while its single-shot capability will benefit the investigation of dynamical processes with pulsed sources, such as X-ray free-electron lasers.

  16. A review of iterative phase retrieval for measurement and encryption

    NASA Astrophysics Data System (ADS)

    Guo, Cheng; Wei, Ce; Tan, Jiubin; Chen, Kana; Liu, Shutian; Wu, Qun; Liu, Zhengjun

    2017-02-01

    Phase retrieval technique is regarded as one of the most significant tools to solve optical inverse problems. Several phase retrieval algorithms are discussed in this review. The occurrence of ill-posed conditions often makes the calculation difficult. As a synthesis, the multiple-image phase retrieval technology is invented to obtain more accurate convergence result in iterative computation. The multiple-input retrieval scheme can attach new constraints on convergence as a new limitation. As an indirect measuring method, it will make it possible to reconstruct the distribution of intensity and phase in an imaging or measurement system, where data processing is executed by computer. Moreover, the retrieval method has been applied for image encryption successfully. Finally, the development and application of the iterative phase retrieval are overviewed.

  17. Improving Performance Support Systems through Information Retrieval Evaluation

    ERIC Educational Resources Information Center

    Schatz, Steven

    2006-01-01

    This study examines existent and new methods for evaluating the success of information retrieval systems. The theory underlying current methods is not robust enough to allow testing retrieval using different meta-tagging schema's. Traditional measures rely on judgments of whether a document is relevant to a particular question. A good system…

  18. Iterative Algorithms for Ptychographic Phase Retrieval

    SciTech Connect

    Yang, Chao; Qian, Jianliang; Schirotzek, Andre; Maia, Filipe; Marchesini, Stefano

    2011-05-03

    Ptychography promises diffraction limited resolution without the need for high resolution lenses. To achieve high resolution one has to solve the phase problem for many partially overlapping frames. Here we review some of the existing methods for solving ptychographic phase retrieval problem from a numerical analysis point of view, and propose alternative methods based on numerical optimization.

  19. A linear phase retrieval wave-front sensor

    NASA Astrophysics Data System (ADS)

    Li, Min; Li, Xin-Yang; Jiang, Wen-Han

    2008-12-01

    A method to retrieve small phase aberration from a single far-field image is proposed. It only needs to calibrate the inherent aberration of the imaging system once, and then the difference between a single measured image with aberration and the calibrated image with inherent aberration is got to retrieve the disturbed phase aberration by an approximate linear relationship. Computer simulations are employed to analyze the performance of this linear phase retrieval (LPR) wave-front sensor. The dynamic range of this method is discussed without noise to judge how small it is needed to satisfy the method. The results show that the proposed small phase retrieval method works well when the RMS phase error is less than 1.6 rad. The Linear Phase Retrieval wave-front sensor and the Hartmann-Shack wave-front sensor are compared on the same stochastic wave-front aberration. The influence of different calculation condition on the retrieval results is compared and analyzed. After analyzing the target resolution, it is thought that a reasonable target size is advantageous to the retrieval precision. At the same time, the LPR sensor can realize the alike precision measurement by using less detect cell, such as 8 pixelx8 pixel in our experiment. From the retrieval results of different orders, the error rate are less than 0.25 and it is comparatively accurate to retrieve pre-35 order aberrations.

  20. Mobile medical image retrieval

    NASA Astrophysics Data System (ADS)

    Duc, Samuel; Depeursinge, Adrien; Eggel, Ivan; Müller, Henning

    2011-03-01

    Images are an integral part of medical practice for diagnosis, treatment planning and teaching. Image retrieval has gained in importance mainly as a research domain over the past 20 years. Both textual and visual retrieval of images are essential. In the process of mobile devices becoming reliable and having a functionality equaling that of formerly desktop clients, mobile computing has gained ground and many applications have been explored. This creates a new field of mobile information search & access and in this context images can play an important role as they often allow understanding complex scenarios much quicker and easier than free text. Mobile information retrieval in general has skyrocketed over the past year with many new applications and tools being developed and all sorts of interfaces being adapted to mobile clients. This article describes constraints of an information retrieval system including visual and textual information retrieval from the medical literature of BioMedCentral and of the RSNA journals Radiology and Radiographics. Solutions for mobile data access with an example on an iPhone in a web-based environment are presented as iPhones are frequently used and the operating system is bound to become the most frequent smartphone operating system in 2011. A web-based scenario was chosen to allow for a use by other smart phone platforms such as Android as well. Constraints of small screens and navigation with touch screens are taken into account in the development of the application. A hybrid choice had to be taken to allow for taking pictures with the cell phone camera and upload them for visual similarity search as most producers of smart phones block this functionality to web applications. Mobile information access and in particular access to images can be surprisingly efficient and effective on smaller screens. Images can be read on screen much faster and relevance of documents can be identified quickly through the use of images contained in

  1. Language Processing in Information Retrieval.

    ERIC Educational Resources Information Center

    Doszkocs, Tamase

    1986-01-01

    Examines role and contributions of natural-language processing in information retrieval and artificial intelligence research in context of large operational information retrieval systems and services. State-of-the-art information retrieval systems combining the functional capabilities of conventional inverted file term adjacency approach with…

  2. Memory Retrieval as Temporal Discrimination

    ERIC Educational Resources Information Center

    Brown, Gordon D. A.; Vousden, Janet I.; McCormack, Teresa

    2009-01-01

    Temporal distinctiveness models of memory retrieval claim that memories are organised partly in terms of their positions along a temporal dimension, and suggest that memory retrieval involves temporal discrimination. According to such models the retrievability of memories should be related to the discriminability of their temporal distances at the…

  3. A Visual Information Retrieval Tool.

    ERIC Educational Resources Information Center

    Zhang, Jin

    2000-01-01

    Discussion of visualization for information retrieval, that transforms unseen internal semantic representation of a document collection into visible geometric displays, focuses on DARE (Distance Angle Retrieval Environment). Highlights include expression of information need; interpretation and manipulation of information retrieval models; ranking…

  4. Human seroreactivity to gut microbiota antigens

    PubMed Central

    Christmann, Benjamin S.; Abrahamsson, Thomas R.; Bernstein, Charles N.; Duck, L. Wayne; Mannon, Peter J.; Berg, Göran; Björkstén, Bengt; Jenmalm, Maria C.; Elson, Charles O.

    2015-01-01

    Background While immune responses directed against antigens from the intestinal microbiota are observed in certain diseases, the normal human adaptive immune response to intestinal microbiota is poorly defined. Objective Our goal was to assess the adaptive immune response to the intestinal microbiota present in 143 healthy adults and compare this response to the immune response observed in 52 children and their mothers at risk of having allergic disease. Methods Human serum was collected from adults and from children followed from birth to seven years of age, and the serum IgG response to a panel of intestinal microbiota antigens was assessed using a novel protein microarray. Results Nearly every individual tested, regardless of health status, had serum IgG that recognized a common set of antigens. Seroreactivity to the panel of antigens was significantly lower in atopic adults. Healthy infants expressed the highest level of IgG seroreactivity to intestinal microbiota antigens. This adaptive response developed between 6 and 12 months of age, and peaked around 2 years of age. Low IgG responses to certain clusters of microbiota antigens during infancy were associated with allergy development during childhood. Conclusions There is an observed perturbation of the adaptive response to antigens from the microbiota in allergic individuals. These perturbations are observable even in childhood, suggesting that optimal stimulation of the adaptive immune system by the microbiota may be needed to prevent certain immune-mediated diseases. PMID:26014812

  5. Antigen injection (image)

    MedlinePlus

    Leprosy is caused by the organism Mycobacterium leprae . The leprosy test involves injection of an antigen just under ... if your body has a current or recent leprosy infection. The injection site is labeled and examined ...

  6. Initial retrieval shields against retrieval-induced forgetting.

    PubMed

    Racsmány, Mihály; Keresztes, Attila

    2015-01-01

    Testing, as a form of retrieval, can enhance learning but it can also induce forgetting of related memories, a phenomenon known as retrieval-induced forgetting (RIF). In four experiments we explored whether selective retrieval and selective restudy of target memories induce forgetting of related memories with or without initial retrieval of the entire learning set. In Experiment 1, subjects studied category-exemplar associations, some of which were then either restudied or retrieved. RIF occurred on a delayed final test only when memories were retrieved and not when they were restudied. In Experiment 2, following the study phase of category-exemplar associations, subjects attempted to recall all category-exemplar associations, then they selectively retrieved or restudied some of the exemplars. We found that, despite the huge impact on practiced items, selective retrieval/restudy caused no decrease in final recall of related items. In Experiment 3, we replicated the main result of Experiment 2 by manipulating initial retrieval as a within-subject variable. In Experiment 4 we replicated the main results of the previous experiments with non-practiced (Nrp) baseline items. These findings suggest that initial retrieval of the learning set shields against the forgetting effect of later selective retrieval. Together, our results support the context shift theory of RIF.

  7. Endoscopic retrieval of gastric trichophytobezoar

    PubMed Central

    Zhao, Jiu-ling; Zhao, Wei-chuan; Wang, Yu-shui

    2017-01-01

    Abstract Rationale: Trichophytobezoars, which are composed of hair and plant fibers, are usually located in the stomach. They are often associated with trichophagia and trichotillomania. The most commonly reported methods of trichophytobezoar treatment are open surgery and laparoscopic retrieval; there are few reports of endoscopic removal of trichophytobezoars. Patient concerns and Diagnoses: Twelve-year-old girl presented with a 3-day history of increasing upper abdominal pain, anorexia, and postprandial emesis. She had a 3-year history of pulling out and eating her own hair. Endoscopic examination showed a large intragastric trichophytobezoar measuring 10.5 cm × 3.5 cm in size, with extension of a few hairs through the pylorus. Interventions and Outcomes: The trichophytobezoar was packed with hair fibers and contained a hard core of mixed hair and vegetable fibers. After the core was cut, the trichophytobezoar was fragmented into pieces with the alternating use of a polypectomy snare and argon plasma coagulation. A small amount of hair and nondigestible food fibers was removed with grasping forceps during the initial procedure. The remaining hairball was loosened with biopsy forceps and was injected with sodium bicarbonate solution. The trichophytobezoar was removed completely at repeat endoscopy 5 days later. After 6 months of psychological intervention, the patient had no recurrence of trichophagia or trichophytobezoar. Lessons: Endoscopy with sodium bicarbonate injection is an effective and minimally invasive method of retrieving a gastric trichophytobezoar. PMID:28099364

  8. DOLPHIn—Dictionary Learning for Phase Retrieval

    NASA Astrophysics Data System (ADS)

    Tillmann, Andreas M.; Eldar, Yonina C.; Mairal, Julien

    2016-12-01

    We propose a new algorithm to learn a dictionary for reconstructing and sparsely encoding signals from measurements without phase. Specifically, we consider the task of estimating a two-dimensional image from squared-magnitude measurements of a complex-valued linear transformation of the original image. Several recent phase retrieval algorithms exploit underlying sparsity of the unknown signal in order to improve recovery performance. In this work, we consider such a sparse signal prior in the context of phase retrieval, when the sparsifying dictionary is not known in advance. Our algorithm jointly reconstructs the unknown signal - possibly corrupted by noise - and learns a dictionary such that each patch of the estimated image can be sparsely represented. Numerical experiments demonstrate that our approach can obtain significantly better reconstructions for phase retrieval problems with noise than methods that cannot exploit such "hidden" sparsity. Moreover, on the theoretical side, we provide a convergence result for our method.

  9. Passage Retrieval and Evaluation

    DTIC Science & Technology

    2005-02-01

    queries, is IDF(t) = log „ # docs. in corpus + 1 0.5 + # docs. t appears in « . (6) This rather ad hoc scoring formula is implemented in the Lemur ...problems with passage R-precision as an evaluation metric. Fernando was instrumental in extending the Lemur toolkit to make all of these experiments...and C. Zhai. The lemur toolkit for language modeling and information retrieval. http://www.cs.cmu.edu/∼ lemur /, 2003. [6] C. Buckley and E. M

  10. REMOTE RETRIEVING TOOL

    DOEpatents

    Fromm, L.W. Jr.

    1958-08-19

    A retrieving tool is described to securely grasp an object for emplacement in, or withdrawal from, an elongated tube. The object is grasped by hooks actuated by a wedge and cann mechanism. The mechanism on the end of a long rodlike structure is controlled by levers or bars at the access end of the tube. This device is particularly useful for positioning fuel elements within a reactor core.

  11. Testing a novel μ-Raman method to retrieve refractive index and density field from femtosecond laser-written optical waveguides

    NASA Astrophysics Data System (ADS)

    Tejerina, M. R.; Torchia, G. A.

    2016-11-01

    In this work, we analysed the refractive index field and other local material properties of femtosecond laser-written waveguides properly combining a novel and direct Raman strategy, waveguides coupling and instrumented nano-indentation. At first, we measured a 2D Raman map within the cross section of femtosecond laser-written waveguides. Then, the Raman Phonon Deformation Constants of lithium niobate were employed to retrieve the strain and density variation from the A1(TO) phonon shifts in the analysed region. We test the results obtained with different combinations of phonons by computing the numerical guided modes and comparing them with those experimentally measured. As a relevant finding, we found that the combination of the A1(TO)1 and A1(TO)4 phonon shifting is the most proper one to compute strain, density and refractive index variation, almost in this kind of waveguide. Finally, a linear path across waveguides cross section was explored with instrumented nano-indentation and the expected variation of local density was detected through a softening of the elastic module observed in the region directly modified by the ultra-fast laser.

  12. Kansei image retrieval based on region of interest

    NASA Astrophysics Data System (ADS)

    Lu, Wei; Ni, Lin

    2005-10-01

    Kansei image retrieval is a new kind of retrieval technology with high complexity. However, it's likely that only some parts of the image would attract people and produce affections. Color imposes a great impact upon the feeling as the basic feature of image, and the entropy of the image also exhibits the information quantity. In this paper, we present a method of kansei image retrieval utilizing the color and entropy to extract regions of interest (ROI). Back propagation neural network is employed to map the color and entropy of ROI to affective feature space. Finally, we show some experimental results of ROI extraction and kansei image retrieval based on interest.

  13. Immunohistochemical localization of procollagens. I. Light microscopic distribution of procollagen I, III and IV antigenicity in the rat incisor tooth by the indirect peroxidase-anti-peroxidase method.

    PubMed

    Cournil, I; Leblond, C P; Pomponio, J; Hand, A R; Sederlof, L; Martin, G R

    1979-07-01

    Frozen sections of the growing end of the rat incisor tooth were exposed to antisera or affinity prepared antibodies against partially purified type I, II, or IV procollagen in the hope of detecting the location of the corresponding antigens by the peroxidase-anti-peroxidase technique. The distribution of immunostaining was similar with antisera as with purified antibodies of a given type, but differed for each type; that is, predentin, odontoblasts, pulp and periodontal tissue were the sites of type I; blood vessel walls, pulp and periodontal tissue, of type III; and basement membranes, of type IV antigenicity. It was demonstrated, at least in cases of type I and III, that immunostaining detected the corresponding procollagens and related substances, but not the corresponding collagens. The interpretation of these observations is that: 1) odontoblasts elaborate procollagen I for release to predentin and subsequent transformation to dentinal collagen I; 2) pulp and periodontal cells produce procollagens I and III which presumably become collagens I and III respectively, while the adventitial cells of blood vessels give rise to collagen III; and 3) procollagen IV is associated with basement membranes and, occasionally, adjacent cells.

  14. Biomedical information retrieval across languages.

    PubMed

    Daumke, Philipp; Markü, Kornél; Poprat, Michael; Schulz, Stefan; Klar, Rüdiger

    2007-06-01

    This work presents a new dictionary-based approach to biomedical cross-language information retrieval (CLIR) that addresses many of the general and domain-specific challenges in current CLIR research. Our method is based on a multilingual lexicon that was generated partly manually and partly automatically, and currently covers six European languages. It contains morphologically meaningful word fragments, termed subwords. Using subwords instead of entire words significantly reduces the number of lexical entries necessary to sufficiently cover a specific language and domain. Mediation between queries and documents is based on these subwords as well as on lists of word-n-grams that are generated from large monolingual corpora and constitute possible translation units. The translations are then sent to a standard Internet search engine. This process makes our approach an effective tool for searching the biomedical content of the World Wide Web in different languages. We evaluate this approach using the OHSUMED corpus, a large medical document collection, within a cross-language retrieval setting.

  15. Iterative phase retrieval without support.

    PubMed

    Wu, J S; Weierstall, U; Spence, J C H; Koch, C T

    2004-12-01

    An iterative phase retrieval method for nonperiodic objects has been developed from the charge-flipping algorithm proposed in crystallography. A combination of the hybrid input-output (HIO) algorithm and the flipping algorithm has greatly improved performance. In this combined algorithm the flipping algorithm serves to find the support (object boundary) dynamically, and the HIO part improves convergence and moves the algorithm out of local minima. It starts with a single intensity measurement in the Fourier domain and does not require a priori knowledge of the support in the image domain. This method is suitable for general image recovery from oversampled diffuse elastic x-ray and electron-diffraction intensities. The relationship between this algorithm and the output-output algorithm is elucidated.

  16. Iterative phase retrieval without support

    NASA Astrophysics Data System (ADS)

    Wu, J. S.; Weierstall, U.; Spence, J. C. H.; Koch, C. T.

    2004-12-01

    An iterative phase retrieval method for nonperiodic objects has been developed from the charge-flipping algorithm proposed in crystallography. A combination of the hybrid input-output (HIO) algorithm and the flipping algorithm has greatly improved performance. In this combined algorithm the flipping algorithm serves to find the support (object boundary) dynamically, and the HIO part improves convergence and moves the algorithm out of local minima. It starts with a single intensity measurement in the Fourier domain and does not require a priori knowledge of the support in the image domain. This method is suitable for general image recovery from oversampled diffuse elastic x-ray and electron-diffraction intensities. The relationship between this algorithm and the output-output algorithm is elucidated.

  17. Use of information-retrieval languages in automated retrieval of experimental data from long-term storage

    NASA Technical Reports Server (NTRS)

    Khovanskiy, Y. D.; Kremneva, N. I.

    1975-01-01

    Problems and methods are discussed of automating information retrieval operations in a data bank used for long term storage and retrieval of data from scientific experiments. Existing information retrieval languages are analyzed along with those being developed. The results of studies discussing the application of the descriptive 'Kristall' language used in the 'ASIOR' automated information retrieval system are presented. The development and use of a specialized language of the classification-descriptive type, using universal decimal classification indices as the main descriptors, is described.

  18. Computer aided selection of candidate vaccine antigens

    PubMed Central

    2010-01-01

    Immunoinformatics is an emergent branch of informatics science that long ago pullulated from the tree of knowledge that is bioinformatics. It is a discipline which applies informatic techniques to problems of the immune system. To a great extent, immunoinformatics is typified by epitope prediction methods. It has found disappointingly limited use in the design and discovery of new vaccines, which is an area where proper computational support is generally lacking. Most extant vaccines are not based around isolated epitopes but rather correspond to chemically-treated or attenuated whole pathogens or correspond to individual proteins extract from whole pathogens or correspond to complex carbohydrate. In this chapter we attempt to review what progress there has been in an as-yet-underexplored area of immunoinformatics: the computational discovery of whole protein antigens. The effective development of antigen prediction methods would significantly reduce the laboratory resource required to identify pathogenic proteins as candidate subunit vaccines. We begin our review by placing antigen prediction firmly into context, exploring the role of reverse vaccinology in the design and discovery of vaccines. We also highlight several competing yet ultimately complementary methodological approaches: sub-cellular location prediction, identifying antigens using sequence similarity, and the use of sophisticated statistical approaches for predicting the probability of antigen characteristics. We end by exploring how a systems immunomics approach to the prediction of immunogenicity would prove helpful in the prediction of antigens. PMID:21067543

  19. A Perspective on the Measurement of Retrieval Effectiveness.

    ERIC Educational Resources Information Center

    Cooper, William S.

    1978-01-01

    Uses an historical analogy with Archimedes' problem concerning the volume of his king's golden crown to illustrate the evaluation problem for document and reference retrieval systems. Various methods that have been developed since 1950 to measure retrieval effectiveness are described, and utility measure formulas are provided. (JD)

  20. The Creation of New Knowledge by Information Retrieval and Classification.

    ERIC Educational Resources Information Center

    Davies, Roy

    1989-01-01

    Reviews previous work on producing knowledge by the use of information retrieval or classification schemes, and describes techniques by which hidden knowledge may be retrieved, i.e., serendipity in browsing and use of appropriate search strategies. Possible future methods based on relational indexing or artificial intelligence are also explored.…

  1. Lensless phase microscopy using phase retrieval with multiple illumination wavelengths.

    PubMed

    Bao, Peng; Situ, Guohai; Pedrini, Giancarlo; Osten, Wolfgang

    2012-08-01

    A phase retrieval method for microscopy using multiple illumination wavelengths is proposed. A fast algorithm suitable for calculations with high numerical aperture is used for the iterative retrieval of the object wavefront. The advantages and limitations of the technique are systematically analyzed and demonstrated by both simulation and experimental results.

  2. Analysis of Search Failures in Document Retrieval Systems: A Review.

    ERIC Educational Resources Information Center

    Tonta, Yasar

    1992-01-01

    Discusses the concept of search failure in document retrieval systems and three effectiveness measures, precision, recall, and "fallout." Four research methods--retrieval effectiveness measures, user satisfaction measures, transaction log analysis, and critical incident technique--are examined, and findings of major studies using each of the…

  3. A Context Vector Model for Information Retrieval.

    ERIC Educational Resources Information Center

    Billhardt, Holger; Borrajo, Daniel; Maojo, Victor

    2002-01-01

    Presents an indexing and information retrieval method that, based on the vector space model, incorporates term dependencies and thus obtains semantically richer representations of documents. Highlights include term context vectors; techniques for estimating the dependencies among terms; term weights; experimental results on four text collections;…

  4. Neural Systems behind Word and Concept Retrieval

    ERIC Educational Resources Information Center

    Damasio, H.; Tranel, D.; Grabowski, T.; Adolphs, R.; Damasio, A.

    2004-01-01

    Using both the lesion method and functional imaging (positron emission tomography) in large cohorts of subjects investigated with the same experimental tasks, we tested the following hypotheses: (A) that the retrieval of words which denote concrete entities belonging to distinct conceptual categories depends upon partially segregated regions in…

  5. Is Retrieval Mediated after Repeated Testing?

    ERIC Educational Resources Information Center

    Kole, James A.; Healy, Alice F.

    2013-01-01

    In 2 main experiments, the mediated priming effect was used to determine whether retrieval continues to be mediated after repeated testing. In each experiment, participants used the keyword method to learn French vocabulary, then completed a modified lexical decision task in which they first translated a French word, and then made a lexical…

  6. A morphological and functional study on antigen binding and endocytosis by immunocytes.

    PubMed Central

    Goud, B; Antoine, J C; Gonatas, N K; Stieber, A; Avrameas, S

    1980-01-01

    Immunoenzymatic techniques were used to study antigen binding and endocytosis by lymph node cells of rats immunized against horseradish peroxidase, hen ovalbumin and rabbit IgG. The number of antigen-binding cells varied and depended on the type of antigen used, the time after immunization, and was higher after a booster injection. In secondary responses (4 days after booster), about 80% of antigen-binding cells were proplasmocytes and plasmocytes; by a double staining procedure it was found that 82% of these cells bore in addition to surface antigen, specific intracytoplasmic antibody as well. About 20% of antigen-binding cells were small and medium lymphocytes which did not contain detectable intracytoplasmic antibody. For ultrastructural studies of the endocytosis, peroxidase was used as the antigen. This antigen was found in cytoplasmic compartments which consisted of vesicles, cisternae and large round bodies (lysosomes?) often located near the Golgi apparatus. However, the cisternae of the Golgi apparatus, involved in the synthesis of specific antibody were not sites of retrieval of endocytosed antigen. The effect of endocytosis of antigen on the secretion and synthesis of antibody was studied by the local haemolysis plaque assay and biosynthetic labelling. No change was detected in antibody secretion and synthesis as a result of antigen endocytosis. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 PMID:7007216

  7. Retrieving Atmospheric Profiles Data in the Presence of Clouds from Hyperspectral Remote Sensing Data

    NASA Technical Reports Server (NTRS)

    Liu, Xu; Larar, Allen M.; Zhou, Daniel K.; Kizer, Susan H.; Wu, Wan; Barnet, Christopher; Divakarla, Murty; Guo, Guang; Blackwell, Bill; Smith, William L.; Yang, Ping; Gu, Degui

    2011-01-01

    Different methods for retrieving atmospheric profiles in the presence of clouds from hyperspectral satellite remote sensing data will be described. We will present results from the JPSS cloud-clearing algorithm and NASA Langley cloud retrieval algorithm.

  8. Music retrieval in ICOR

    NASA Astrophysics Data System (ADS)

    Finsterle, Lutz; Fischer, Stephan; Rimac, Ivica; Steinmetz, Ralf

    1999-08-01

    In this paper we describe music retrieval in ICOR, a project of Darmstadt TU. It is the goal of ICOR to find new interfaces to support applications of music video and music CDs. Although the project consists of audio and video analysis we concentrate on a description of the audio algorithms in this paper. We describe our MPEG-7 like data structure to store meta information for music pieces and explain which algorithms we use to analyze the content of music pieces automatically. We currently use an applause detection to distinguish live music from studio recordings, a genre classifier to distinguish pieces with beats form classical music, and a singer recognition.

  9. 2. VIEW OF INTERIOR OF XY RETRIEVER. THE XY RETRIEVER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW OF INTERIOR OF X-Y RETRIEVER. THE X-Y RETRIEVER WAS HOUSED IN MODULE K AND WAS USED TO SORT AND RETRIEVE PLUTONIUM METAL FROM A STORAGE VAULT FOR DISTRIBUTION TO OTHER PROCESSES IN BUILDING 707. (11/29/88) - Rocky Flats Plant, Plutonium Manufacturing Facility, North-central section of Plant, just south of Building 776/777, Golden, Jefferson County, CO

  10. Difficult OptEase Filter Retrievals After Prolonged Indwelling Times

    SciTech Connect

    Van Ha, Thuong G. Kang, Lisa; Lorenz, Jonathan; Zangan, Steven; Navuluri, Rakesh; Straus, Christopher; Funaki, Brian

    2013-08-01

    PurposeThe OptEase vena cave filter (Cordis, Piscataway, NJ) is commercially available as a retrievable or permanent filter with short recommended indwelling time, presumably due to extensive contact of the filter side struts with the inferior vena cava wall and subsequent neointimal hyperplasia leading to incorporation. Our purpose was to evaluate OptEase filter retrievals with a long indwelling time period that required unconventional retrieval techniques.Materials and MethodsWe retrospectively reviewed patients who underwent OptEase filter retrieval with long undwelling times requiring additional maneuvers for retrieval. Techniques used included rigid endobronchial forceps dissection and wire-through-loop snare. Each patient underwent postretrieval venogram to evaluate for possible complications. In addition, patients had clinical follow-up 2 weeks after the retrieval procedure.ResultsThere were three patients (2 women, 1 man; average age 64 years) who underwent OptEase filter retrieval. The mean indwelling time was 6.4 months. The indwelling filters were successfully retrieved. There were no complications. Postprocedural follow-up showed no clinical pathology.ConclusionUnconventional techniques aided in the retrieval of OptEase filters with long indwelling times.

  11. A novel homogeneous immunoassay for anthrax detection based on the AlphaLISA method: detection of B. anthracis spores and protective antigen (PA) in complex samples.

    PubMed

    Mechaly, Adva; Cohen, Noam; Weiss, Shay; Zahavy, Eran

    2013-05-01

    Amplified Luminescent Proximity Homogeneous Assay (AlphaLISA) technology is an energy-transfer-based assay, utilizing singlet oxygen as an energy donor to a fluorescent acceptor. The long singlet oxygen migration distance allows the energy transfer mechanism to go up to ~200 nm, facilitating flexible and sensitive homogeneous immunoassays. While soluble protein detection using AlphaLISA was previously described, the detection of particles such as bacteria and viruses was not reported. In this work, we show for the first time the implementation of the AlphaLISA technology for the detection of a particulate antigen, i.e., Bacillus anthracis spores. Here, we show that an efficient particle immunoassay requires a high acceptor-to-donor ratio (>4:1). The results suggested that the high acceptor/donor ratio is required to avoid donor aggregation ("islands") on the spore surface, hence facilitating donor/acceptor interaction. The developed assay enabled the detection of 10(6) spores/mL spiked in PBS. We also demonstrate the development of a highly sensitive AlphaLISA assay for the detection of the main toxin component of anthrax, protective antigen (PA). The assay enabled the detection of 10 and 100 pg/mL PA in buffer and spiked naïve rabbit sera, respectively, and was successfully implemented in sera of anthrax-infected rabbits. To summarize, this study demonstrates that AlphaLISA enables detection of anthrax spores and toxin, utilizing short homogeneous assays. Moreover, it is shown for the first time that this technology facilitates the detection of particulate entities and might be suitable for the detection of other bacteria or viruses.

  12. Comparison of blood smear, antigen detection, and nested-PCR methods for screening refugees from regions where malaria is endemic after a malaria outbreak in Quebec, Canada.

    PubMed

    Ndao, Momar; Bandyayera, Etienne; Kokoskin, Evelyne; Gyorkos, Theresa W; MacLean, J Dick; Ward, Brian J

    2004-06-01

    The importation of malaria into a region where it is not endemic raises many concerns, including the timely delivery of appropriate care, safety of the blood supply, and the risk of autochthonous transmission. There is presently no consensus on the best way to screen mobile populations for malaria. Between August 2000 and March 2001, 535 refugees arrived in Quebec, Canada, from Tanzanian camps. Within 4 weeks of resettlement of the first group of 224, the McGill University Centre for Tropical Diseases noted an outbreak of malaria across the province (15 cases over a 3-week period). This group (group 1) was traced and screened for malaria between 3 and 4 months after arrival in Canada. Subsequent groups of 106 and 205 refugees were screened immediately upon arrival in Canada (group 2) and immediately prior to their departure from refugee camps (group 3), respectively. A single EDTA-blood sample was obtained from 521 refugees for testing by thick and thin blood smears (groups 1 and 2), antigen detection (ICT Malaria Pf and OptiMAL; group 1 only), and nested PCR (all groups). Overall, 98 of 521 refugees were found to be infected (18.8%). The vast majority of infections (81 of 98) were caused by Plasmodium falciparum alone. Using PCR as the "gold standard," both microscopy (sensitivity, 50%; specificity, 100%) and antigen detection (ICT sensitivity, 37.5%; ICT specificity, 100%; OptiMAL sensitivity, 29.1%; OptiMAL specificity, 95.6%) performed poorly. None of the PCR-positive subjects were symptomatic at the time of testing, and only two had recently had symptoms compatible with malaria (with or without diagnosis and treatment). Active surveillance of migrants from regions of intense malaria transmission can reduce the risk of morbidity in the migrant population and mitigate against transmission to the host population. Our data demonstrate that PCR is, by far, the most powerful tool for such surveillance.

  13. Multiple image encryption by phase retrieval

    NASA Astrophysics Data System (ADS)

    Di, Hong; Kang, Yanmei; Liu, Yueqin; Zhang, Xin

    2016-07-01

    Multiple image encryption (MIE) was proposed to increase the efficiency of encrypting images by processing several images simultaneously. Because of the advantage of optical technology in processing twodimensional images at high throughput, MIE has been significantly improved by use of methods originating from optics. Phase retrieval was the process of algorithmically finding solutions to the phase loss problem due to light detectors only capturing the intensity. It was to retrieve phase information for the determination of a structure from diffraction data. Error-reduction algorithm is a typical phase retrieval method. Here, we employ it to illustrate that methods in phase retrieval are able to encrypt multiple images and compress them into encrypted data simultaneously. Moreover, the decryption is also designed to handle multiple images at the same time. The whole process including both the encryption and decryption is proposed to improve MIE with respect to the compression and efficiency. The feasibility and encryption of the MIE scheme is demonstrated with encryption experiments under Gaussian white noise and unauthorized access.

  14. Diagnostic Antigens of Leishmania.

    DTIC Science & Technology

    1994-01-31

    braziliensis (MHOM/BR/75/M2903), L. chagasi (MJOM/BR/82/BA-2,C 1), L. donovani (MHOMiEt/67iHU3), Leishmania guyanensis (MIHOMJBR/75/M4147), L. infantum (IPT-1...comparative test to a variety of other recombinant Leishmania antigens including L. chagasi hsp70, L. braziliensis hsp83/90, L. braziliensis eIF4A, L...34 4. AD CONTRACT NO: DAMD17-92-C-2082 EC•£ 2 j 994 ’i, L TITLE: DIAGNOSTIC ANTIGENS OF LEISHMANIA L PRINCIPAL INVESTIGATOR: Steven G. Reed, Ph.D

  15. The Text Retrieval Conferences (TRECs)

    DTIC Science & Technology

    1996-05-01

    Retrieval and Routing Using the INQUERY System" by John Broglio, James P. Callan, W. Bruce Croft and Daniel W. Nachbar) used a version of...University of Massachusetts at Amherst ("Document Retrieval and Routing Using the INQUERY System" by John Broglio, James P. Callan, W. Bruce Croft...342 terms were used. SIN002, SINOOI -- University of Massachusetts at Amherst ("Document Retrieval and Routing Using the INQUERY System" by John

  16. Hetero-organic thymus antigens.

    PubMed

    Beletskaya, L V; Gnezditskaya, E V

    1985-01-01

    The use of sera containing antibodies to tissue-specific antigens of highly specialized organs (skeletal muscles, heart, skin, excretory glands) enabled us to detect, by immunofluorescence, cells capable of synthesizing analogous antigens (i.e. hetero-organic thymus antigens) in human and animal thymus. Detection of hetero-organic antigens in the thymus is the basis for the hypothesis that natural immunological tolerance to tissue self antigens is formed within the thymus in the course of T-lymphocyte maturation, with thymus antigens taking part in the process.

  17. EM-21 Retrieval Knowledge Center: Waste Retrieval Challenges

    SciTech Connect

    Fellinger, Andrew P.; Rinker, Michael W.; Berglin, Eric J.; Minichan, Richard L.; Poirier, Micheal R.; Gauglitz, Phillip A.; Martin, Bruce A.; Hatchell, Brian K.; Saldivar, Eloy; Mullen, O Dennis; Chapman, Noel F.; Wells, Beric E.; Gibbons, Peter W.

    2009-04-10

    EM-21 is the Waste Processing Division of the Office of Engineering and Technology, within the U.S. Department of Energy’s (DOE) Office of Environmental Management (EM). In August of 2008, EM-21 began an initiative to develop a Retrieval Knowledge Center (RKC) to provide the DOE, high level waste retrieval operators, and technology developers with centralized and focused location to share knowledge and expertise that will be used to address retrieval challenges across the DOE complex. The RKC is also designed to facilitate information sharing across the DOE Waste Site Complex through workshops, and a searchable database of waste retrieval technology information. The database may be used to research effective technology approaches for specific retrieval tasks and to take advantage of the lessons learned from previous operations. It is also expected to be effective for remaining current with state-of-the-art of retrieval technologies and ongoing development within the DOE Complex. To encourage collaboration of DOE sites with waste retrieval issues, the RKC team is co-led by the Savannah River National Laboratory (SRNL) and the Pacific Northwest National Laboratory (PNNL). Two RKC workshops were held in the Fall of 2008. The purpose of these workshops was to define top level waste retrieval functional areas, exchange lessons learned, and develop a path forward to support a strategic business plan focused on technology needs for retrieval. The primary participants involved in these workshops included retrieval personnel and laboratory staff that are associated with Hanford and Savannah River Sites since the majority of remaining DOE waste tanks are located at these sites. This report summarizes and documents the results of the initial RKC workshops. Technology challenges identified from these workshops and presented here are expected to be a key component to defining future RKC-directed tasks designed to facilitate tank waste retrieval solutions.

  18. The potential of clear-sky carbon dioxide satellite retrievals

    NASA Astrophysics Data System (ADS)

    Nelson, Robert R.; O'Dell, Christopher W.; Taylor, Thomas E.; Mandrake, Lukas; Smyth, Mike

    2016-04-01

    ">2 root-mean-square errors (RMSEs) of less than 2.0 ppm, relative to Total Carbon Column Observing Network (TCCON) measurements and a suite of CO2 models, when adequately filtered through the use of a custom genetic algorithm filtering system. These results imply that non-scattering XCO2 retrievals are potentially more useful than previous literature suggests, as the filtering methods we employ are able to remove measurements in which scattering can cause significant errors. Additionally, the computational benefits of non-scattering retrievals means they may be useful for certain applications that require large amounts of data but have less stringent error requirements.

  19. Semantic processing in information retrieval.

    PubMed Central

    Rindflesch, T. C.; Aronson, A. R.

    1993-01-01

    Intuition suggests that one way to enhance the information retrieval process would be the use of phrases to characterize the contents of text. A number of researchers, however, have noted that phrases alone do not improve retrieval effectiveness. In this paper we briefly review the use of phrases in information retrieval and then suggest extensions to this paradigm using semantic information. We claim that semantic processing, which can be viewed as expressing relations between the concepts represented by phrases, will in fact enhance retrieval effectiveness. The availability of the UMLS domain model, which we exploit extensively, significantly contributes to the feasibility of this processing. PMID:8130547

  20. Care episode retrieval: distributional semantic models for information retrieval in the clinical domain

    PubMed Central

    2015-01-01

    Patients' health related information is stored in electronic health records (EHRs) by health service providers. These records include sequential documentation of care episodes in the form of clinical notes. EHRs are used throughout the health care sector by professionals, administrators and patients, primarily for clinical purposes, but also for secondary purposes such as decision support and research. The vast amounts of information in EHR systems complicate information management and increase the risk of information overload. Therefore, clinicians and researchers need new tools to manage the information stored in the EHRs. A common use case is, given a - possibly unfinished - care episode, to retrieve the most similar care episodes among the records. This paper presents several methods for information retrieval, focusing on care episode retrieval, based on textual similarity, where similarity is measured through domain-specific modelling of the distributional semantics of words. Models include variants of random indexing and the semantic neural network model word2vec. Two novel methods are introduced that utilize the ICD-10 codes attached to care episodes to better induce domain-specificity in the semantic model. We report on experimental evaluation of care episode retrieval that circumvents the lack of human judgements regarding episode relevance. Results suggest that several of the methods proposed outperform a state-of-the art search engine (Lucene) on the retrieval task. PMID:26099735

  1. Care episode retrieval: distributional semantic models for information retrieval in the clinical domain.

    PubMed

    Moen, Hans; Ginter, Filip; Marsi, Erwin; Peltonen, Laura-Maria; Salakoski, Tapio; Salanterä, Sanna

    2015-01-01

    Patients' health related information is stored in electronic health records (EHRs) by health service providers. These records include sequential documentation of care episodes in the form of clinical notes. EHRs are used throughout the health care sector by professionals, administrators and patients, primarily for clinical purposes, but also for secondary purposes such as decision support and research. The vast amounts of information in EHR systems complicate information management and increase the risk of information overload. Therefore, clinicians and researchers need new tools to manage the information stored in the EHRs. A common use case is, given a--possibly unfinished--care episode, to retrieve the most similar care episodes among the records. This paper presents several methods for information retrieval, focusing on care episode retrieval, based on textual similarity, where similarity is measured through domain-specific modelling of the distributional semantics of words. Models include variants of random indexing and the semantic neural network model word2vec. Two novel methods are introduced that utilize the ICD-10 codes attached to care episodes to better induce domain-specificity in the semantic model. We report on experimental evaluation of care episode retrieval that circumvents the lack of human judgements regarding episode relevance. Results suggest that several of the methods proposed outperform a state-of-the art search engine (Lucene) on the retrieval task.

  2. Antigen detection systems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Infectious agents or their constituent parts (antigens or nucleic acids) can be detected in fresh, frozen, or fixed tissues or other specimens, using a variety of direct or indirect assays. The assays can be modified to yield the greatest sensitivity and specificity but in most cases a particular m...

  3. Antigen smuggling in tuberculosis.

    PubMed

    Hudrisier, Denis; Neyrolles, Olivier

    2014-06-11

    The importance of CD4 T lymphocytes in immunity to M. tuberculosis is well established; however, how dendritic cells activate T cells in vivo remains obscure. In this issue of Cell Host & Microbe, Srivastava and Ernst (2014) report a mechanism of antigen transfer for efficient activation of antimycobacterial T cells.

  4. Antigen detection systems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Infectious agents or their constituent parts (antigens or nucleic acids) can be detected in fresh, frozen, or fixed tissue using a variety of direct or indirect assays. The assays can be modified to yield the greatest sensitivity and specificity but in most cases a particular methodology is chosen ...

  5. Improvement of MODIS aerosol retrievals near clouds

    NASA Astrophysics Data System (ADS)

    Wen, Guoyong; Marshak, Alexander; Levy, Robert C.; Remer, Lorraine A.; Loeb, Norman G.; Várnai, Tamás.; Cahalan, Robert F.

    2013-08-01

    retrieval of aerosol properties near clouds from reflected sunlight is challenging. Sunlight reflected from clouds can effectively enhance the reflectance in nearby clear regions. Ignoring cloud 3-D radiative effects can lead to large biases in aerosol retrievals, risking an incorrect interpretation of satellite observations on aerosol-cloud interaction. Earlier, we developed a simple model to compute the cloud-induced clear-sky radiance enhancement that is due to radiative interaction between boundary layer clouds and the molecular layer above. This paper focuses on the application and implementation of the correction algorithm. This is the first time that this method is being applied to a full Moderate Resolution Imaging Spectroradiometer (MODIS) granule. The process of the correction includes converting Clouds and the Earth's Radiant Energy System broadband flux to visible narrowband flux, computing the clear-sky radiance enhancement, and retrieving aerosol properties. We find that the correction leads to smaller values in aerosol optical depth (AOD), Ångström exponent, and the small mode aerosol fraction of the total AOD. It also makes the average aerosol particle size larger near clouds than far away from clouds, which is more realistic than the opposite behavior observed in operational retrievals. We discuss issues in the current correction method as well as our plans to validate the algorithm.

  6. Population Based Assessment of MHC Class 1 Antigens Down Regulation as Marker in Increased Risk for Development and Progression of Breast Cancer From Benign Breast Lesions

    DTIC Science & Technology

    2006-01-01

    Risk for Development and Progression of Breast Cancer from Benign Breast Lesions” antigen retrieval solution in the microwave . Lastly, tissue... Microwave and citrate buffer antigen-retrieval substitutions yielded comparable results. Lab 2 demonstrated that a negative control is useful as an...the infiltrating lymphocytes in germinal centers and dendritic cells as seen in case 1. Microsatellite Analysis Labs 1, 2, and 4 performed

  7. Multilevel resistive information storage and retrieval

    DOEpatents

    Lohn, Andrew; Mickel, Patrick R.

    2016-08-09

    The present invention relates to resistive random-access memory (RRAM or ReRAM) systems, as well as methods of employing multiple state variables to form degenerate states in such memory systems. The methods herein allow for precise write and read steps to form multiple state variables, and these steps can be performed electrically. Such an approach allows for multilevel, high density memory systems with enhanced information storage capacity and simplified information retrieval.

  8. Bayesian nonparametric clustering in phylogenetics: modeling antigenic evolution in influenza.

    PubMed

    Cybis, Gabriela B; Sinsheimer, Janet S; Bedford, Trevor; Rambaut, Andrew; Lemey, Philippe; Suchard, Marc A

    2017-01-18

    Influenza is responsible for up to 500,000 deaths every year, and antigenic variability represents much of its epidemiological burden. To visualize antigenic differences across many viral strains, antigenic cartography methods use multidimensional scaling on binding assay data to map influenza antigenicity onto a low-dimensional space. Analysis of such assay data ideally leads to natural clustering of influenza strains of similar antigenicity that correlate with sequence evolution. To understand the dynamics of these antigenic groups, we present a framework that jointly models genetic and antigenic evolution by combining multidimensional scaling of binding assay data, Bayesian phylogenetic machinery and nonparametric clustering methods. We propose a phylogenetic Chinese restaurant process that extends the current process to incorporate the phylogenetic dependency structure between strains in the modeling of antigenic clusters. With this method, we are able to use the genetic information to better understand the evolution of antigenicity throughout epidemics, as shown in applications of this model to H1N1 influenza. Copyright © 2017 John Wiley & Sons, Ltd.

  9. Assessment of Snared-Loop Technique When Standard Retrieval of Inferior Vena Cava Filters Fails

    SciTech Connect

    Doody, Orla Noe, Geertje; Given, Mark F.; Foley, Peter T.; Lyon, Stuart M.

    2009-01-15

    Purpose To identify the success and complications related to a variant technique used to retrieve inferior vena cava filters when simple snare approach has failed. Methods A retrospective review of all Cook Guenther Tulip filters and Cook Celect filters retrieved between July 2006 and February 2008 was performed. During this period, 130 filter retrievals were attempted. In 33 cases, the standard retrieval technique failed. Retrieval was subsequently attempted with our modified retrieval technique. Results The retrieval was successful in 23 cases (mean dwell time, 171.84 days; range, 5-505 days) and unsuccessful in 10 cases (mean dwell time, 162.2 days; range, 94-360 days). Our filter retrievability rates increased from 74.6% with the standard retrieval method to 92.3% when the snared-loop technique was used. Unsuccessful retrieval was due to significant endothelialization (n = 9) and caval penetration by the filter (n = 1). A single complication occurred in the group, in a patient developing pulmonary emboli after attempted retrieval. Conclusion The technique we describe increased the retrievability of the two filters studied. Hook endothelialization is the main factor resulting in failed retrieval and continues to be a limitation with these filters.

  10. Intelligent Information Retrieval: An Introduction.

    ERIC Educational Resources Information Center

    Gauch, Susan

    1992-01-01

    Discusses the application of artificial intelligence to online information retrieval systems and describes several systems: (1) CANSEARCH, from MEDLINE; (2) Intelligent Interface for Information Retrieval (I3R); (3) Gausch's Query Reformulation; (4) Environmental Pollution Expert (EP-X); (5) PLEXUS (gardening); and (6) SCISOR (corporate…

  11. Team 2: AIRS Only Retrieval

    NASA Technical Reports Server (NTRS)

    Lee, Sung-Yung; Manning, Evan; Blaisdell, John; Susskind, Joel; Barnet, Chris; Goldberg, Mitch; Cho, Chuck; Staelin, Dave; Blackwelll, Bill

    2005-01-01

    This slide presentation makes the case for the retrieval of data from the Atmospheric Infrared Sounder (AIRS) Advanced Microwave Sounding Unit (AMSU). AIRS only retrieval is not only a risk reduction for failure of AMSU, but also important because NWP centers are reluctant to assimilate AMSU twice.

  12. Student Attitude Toward Information Retrieval.

    ERIC Educational Resources Information Center

    Adair, Charles H.; Allen, Rodney F.

    This is an individually administered rating scale designed to evaluate teacher trainee attitudes toward an information retrieval system. A major goal of the scale is to seek responses that measure students' reactions to the cognitive interest and motivational nature of the information retrieval system through the use of Likert-type items. The…

  13. Ubiquitous Home: Retrieval of Experiences in a Home Environment

    NASA Astrophysics Data System (ADS)

    Silva, Gamhewage C. De; Yamasaki, Toshihiko; Aizawa, Kiyoharu

    Automated capture and retrieval of experiences at home is interesting due to the wide variety and personal significance of such experiences. We present a system for retrieval and summarization of continuously captured multimedia data from Ubiquitous Home, a two-room house consisting of a large number of cameras and microphones. Data from pressure based sensors on the floor are analyzed to segment footsteps of different persons. Video and audio handover are implemented to retrieve continuous video streams corresponding to moving persons. An adaptive algorithm based on the rate of footsteps summarizes these video streams. A novel method for audio segmentation using multiple microphones is used for video retrieval based on sounds with high accuracy. An experiment, in which a family lived in this house for twelve days, was conducted. The system was evaluated by the residents who used the system for retrieving their own experiences; we report and discuss the results.

  14. A neural network-based method for merging ocean color and Argo data to extend surface bio-optical properties to depth: Retrieval of the particulate backscattering coefficient

    NASA Astrophysics Data System (ADS)

    Sauzède, R.; Claustre, H.; Uitz, J.; Jamet, C.; Dall'Olmo, G.; D'Ortenzio, F.; Gentili, B.; Poteau, A.; Schmechtig, C.

    2016-04-01

    The present study proposes a novel method that merges satellite ocean color bio-optical products with Argo temperature-salinity profiles to infer the vertical distribution of the particulate backscattering coefficient (bbp). This neural network-based method (SOCA-BBP for Satellite Ocean-Color merged with Argo data to infer the vertical distribution of the Particulate Backscattering coefficient) uses three main input components: (1) satellite-based surface estimates of bbp and chlorophyll a concentration matched up in space and time with (2) depth-resolved physical properties derived from temperature-salinity profiles measured by Argo profiling floats and (3) the day of the year of the considered satellite-Argo matchup. The neural network is trained and validated using a database including 4725 simultaneous profiles of temperature-salinity and bio-optical properties collected by Bio-Argo floats, with concomitant satellite-derived products. The Bio-Argo profiles are representative of the global open-ocean in terms of oceanographic conditions, making the proposed method applicable to most open-ocean environments. SOCA-BBP is validated using 20% of the entire database (global error of 21%). We present additional validation results based on two other independent data sets acquired (1) by four Bio-Argo floats deployed in major oceanic basins, not represented in the database used to train the method; and (2) during an AMT (Atlantic Meridional Transect) field cruise in 2009. These validation tests based on two fully independent data sets indicate the robustness of the predicted vertical distribution of bbp. To illustrate the potential of the method, we merged monthly climatological Argo profiles with ocean color products to produce a depth-resolved climatology of bbp for the global ocean.

  15. An improved glyoxal retrieval from OMI measurements

    NASA Astrophysics Data System (ADS)

    Alvarado, L. M. A.; Richter, A.; Vrekoussis, M.; Wittrock, F.; Hilboll, A.; Schreier, S. F.; Burrows, J. P.

    2014-06-01

    Satellite observations from the SCIAMACHY, GOME-2, and OMI spectrometers have been used to retrieve atmospheric columns of glyoxal (CHOCHO) with the DOAS method. High CHOCHO levels are found over regions with large biogenic and pyrogenic emissions, and hot-spots have been identified over areas of anthropogenic activities. This study focuses on the development of an improved retrieval for CHOCHO from measurements by the OMI instrument. From sensitivity tests, an optimal fitting window and polynomial degree are determined. Two different approaches to reduce the interference of liquid water absorption over oceanic regions are evaluated, achieving significant reduction of negative columns over clear water regions. Moreover, a high temperature absorption cross-section of nitrogen dioxide (NO2) is introduced in the DOAS retrieval to account for potential interferences of NO2 over regions with large anthropogenic emissions, leading to improved fit quality over these areas. A comparison with vertical CHOCHO columns retrieved from measurements of the GOME-2 and SCIAMACHY instruments over continental regions is performed, showing overall good consistency. Using the new OMI CHOCHO data set, the link between fires and glyoxal columns is investigated for two selected regions in Africa. In addition, mapped averages are computed for a fire event in the east of Moscow between mid-July and mid-August 2010. In both cases, enhanced CHOCHO levels are found in close spatial and temporal proximity to MODIS fire radiative power, demonstrating that pyrogenic emissions can be clearly identified in the OMI CHOCHO product.

  16. Retrieval of spatial field of atmospheric aerosol concentration according to data from local measurements: A modification of the method of back trajectory statistics

    NASA Astrophysics Data System (ADS)

    Poddubny, V. A.; Nagovitsyna, E. S.

    2013-07-01

    The well-known method of the back trajectory statistics (BTS) is used to develop a new approach to estimating atmospheric pollution fields according to local measurements. On the basis of instrumental measurements at one or a few monitoring points and information on atmospheric dynamics (in this work, back trajectories of the motion of air particles), the BTS method makes it possible to estimate the spatial structure of fields of a measured quantity. Results from solving a simplified demonstration of estimating the spatial distribution of the volume concentration of a fine aerosol fraction are presented; these results were obtained on the basis of an analysis of photometric measurements in the period of 2004-2010 at six AERO-NET monitoring sites on the territory of Russia: Zvenigorod, Moscow, Yekaterinburg, Tomsk, Yakutsk, and Ussuriisk.

  17. Antigenic evaluation of a recombinant baculovirus-expressed Sarcocystis neurona SAG1 antigen.

    PubMed

    Gupta, G D; Lakritz, J; Saville, W J; Livingston, R S; Dubey, J P; Middleton, J R; Marsh, A E

    2004-10-01

    Sarcocystis neurona is the primary parasite associated with equine protozoal myeloencephalitis (EPM). This is a commonly diagnosed neurological disorder in the Americas that infects the central nervous system of horses. Current serologic assays utilize culture-derived parasites as antigen. This method requires large numbers of parasites to be grown in culture, which is labor intensive and time consuming. Also, a culture-derived whole-parasite preparation contains conserved antigens that could cross-react with antibodies against other Sarcocystis species and members of Sarcocystidae such as Neospora spp., Hammondia spp., and Toxoplasma gondii. Therefore, there is a need to develop an improved method for the detection of S. neurona-specific antibodies. The sera of infected horses react strongly to surface antigen 1 (SnSAG1), an approximately 29-kDa protein, in immunoblot analysis, suggesting that it is an immunodominant antigen. The SnSAG1 gene of S. neurona was cloned, and recombinant S. neurona SAG1 protein (rSnSAG1-Bac) was expressed with the use of a baculovirus system. By immunoblot analysis, the rSnSAG1-Bac antigen detected antibodies to S. neurona from naturally infected and experimentally inoculated equids, cats, rabbit, mice, and skunk. This is the first report of a baculovirus-expressed recombinant S. neurona antigen being used to detect anti-S. neurona antibodies in a variety of host species.

  18. Coproduction of carcinoembryonic antigen and nonspecific cross-reacting antigen by a continuous cell line from a human pancreatic tumor.

    PubMed

    Kuroki, M; Ichiki, S; Kuroki, M; Matsuoka, Y

    1982-08-01

    A simultaneous production of nonspecific cross-reacting antigen (NCA) and carcinoembryonic antigen (CEA) by the same individual cells of an established human pancreatic cell line (QGP-1) was demonstrated by the immunoperoxidase method. Kinetics of cell proliferation and production of CEA and NCA were analyzed, and active synthesis of both antigens was found to be accompanied with the active proliferation of cultured cells. Both antigens in culture medium were purified by immunoadsorption and gel filtration. Immunochemical studies confirmed that CEA and NCA produced by the QGP-1 cells had properties identical to those of authentic CEA derived from metastatic colorectal carcinoma and to those of NCA from normal lungs, respectively.

  19. Phase retrieval of reflectance for nanoparticle optical identification.

    PubMed

    Saarinen, Jarkko J; Uozumi, Jun; Vartiainen, Erik M; Peiponen, Kai-Erik

    2012-06-15

    We present a method for optical identification of dielectric and metal nanoparticles in a liquid matrix using phase retrieval of reflectance with TE- and TM-polarized light. A formula is derived for extracting the effective complex dielectric function of a nanoparticle colloid based on different complex reflectance components. The phase retrieval is performed using the maximum entropy method. We observe excellent accuracy both for dielectric and metallic nanoparticles with volume fractions up to 10%.

  20. The impact of cloud vertical profile on liquid water path retrieval based on the bispectral method: A theoretical study based on large-eddy simulations of shallow marine boundary layer clouds

    NASA Astrophysics Data System (ADS)

    Miller, Daniel J.; Zhang, Zhibo; Ackerman, Andrew S.; Platnick, Steven; Baum, Bryan A.

    2016-04-01

    Passive optical retrievals of cloud liquid water path (LWP), like those implemented for Moderate Resolution Imaging Spectroradiometer (MODIS), rely on cloud vertical profile assumptions to relate optical thickness (τ) and effective radius (re) retrievals to LWP. These techniques typically assume that shallow clouds are vertically homogeneous; however, an adiabatic cloud model is plausibly more realistic for shallow marine boundary layer cloud regimes. In this study a satellite retrieval simulator is used to perform MODIS-like satellite retrievals, which in turn are compared directly to the large-eddy simulation (LES) output. This satellite simulator creates a framework for rigorous quantification of the impact that vertical profile features have on LWP retrievals, and it accomplishes this while also avoiding sources of bias present in previous observational studies. The cloud vertical profiles from the LES are often more complex than either of the two standard assumptions, and the favored assumption was found to be sensitive to cloud regime (cumuliform/stratiform). Confirming previous studies, drizzle and cloud top entrainment of dry air are identified as physical features that bias LWP retrievals away from adiabatic and toward homogeneous assumptions. The mean bias induced by drizzle-influenced profiles was shown to be on the order of 5-10 g/m2. In contrast, the influence of cloud top entrainment was found to be smaller by about a factor of 2. A theoretical framework is developed to explain variability in LWP retrievals by introducing modifications to the adiabatic re profile. In addition to analyzing bispectral retrievals, we also compare results with the vertical profile sensitivity of passive polarimetric retrieval techniques.

  1. Web Mining for Web Image Retrieval.

    ERIC Educational Resources Information Center

    Chen, Zheng; Wenyin, Liu; Zhang, Feng; Li, Mingjing; Zhang, Hongjiang

    2001-01-01

    Presents a prototype system for image retrieval from the Internet using Web mining. Discusses the architecture of the Web image retrieval prototype; document space modeling; user log mining; and image retrieval experiments to evaluate the proposed system. (AEF)

  2. Method for determining the minimum principle horizontal stress within a formation through use of a wireline retrievable circumferential acoustic scanning tool during an open hole microfrac test

    SciTech Connect

    Venditto, J.J.; McMechan, D.E.; Enderlin, M.B.

    1993-08-17

    A method is described for determining the magnitude of the minimum principle horizontal stress within a formation having an open hole well bore formed therein, comprising the steps of: supplying pressurized fracturing fluid to said well bore in a desired area of observation, said well bore exposing an interior surface of said formation; inserting a circumferential acoustic scanning tool into said well bore and positioning said tool in said area of observation, said acoustic scanning tool providing a viewable image of said interior surface of said formation; increasing the pressure on said fracturing fluid until fractures are initiated in said formation proximate the well bore; increasing the pressure acting on said fracturing fluid so as to cause said initiated fractures to further open; observing the interior surface of said formation during said opening of said previously initiated fractures; and noting the magnitude of the pressure acting on said fracturing fluid as said previously initiated fractures are opened.

  3. Antigens provide immunity against Ich in channel catfish trials

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Studies were conducted to determine effects of 1) types of Ich antigens and routes of immunization, 2) methods of inactivated trophonts, and 3) antigen doses on fish immune protection against Ichthyophthirius multifiliis Fouquet (Ich). All catfish immunized with live theronts by immersion, live the...

  4. Research by retrieving experiments.

    PubMed

    Blagosklonny, Mikhail V

    2007-06-01

    Newton did not discover that apples fall: the information was available prior to his gravitational hypothesis. Hypotheses can be tested not only by performing experiments but also by retrieving experiments from the literature (via PubMed, for example). Here I show how disconnected facts from known data, if properly connected, can generate novel predictions testable in turn by other published data. With examples from cell cycle, aging, cancer and other fields of biology and medicine, I discuss how new knowledge was and will be derived from old information. Millions of experiments have been already performed to test unrelated hypotheses and the results of those experiments are available to 'test' your hypotheses too. But most data (99% by some estimates) remain unpublished, because they were negative, seemed of low priority, or did not fit the story. Yet for other investigators those data may be valuable. The well-known story of Franklin and Watson is a case in point. By making preliminary data widely available, 'data-owners' will benefit most, receiving the credit for otherwise unused results. If posted (pre-published) on searchable databases, these data may fuel thousands of projects without the need for repetitive experiments. Enormous 'pre-published' databases coupled with Google-like search engines can change the structure of scientific research, and shrinking funding will make this inevitable.

  5. Solid rocket booster retrieval operations

    NASA Technical Reports Server (NTRS)

    Rasmussen, A. M.

    1985-01-01

    Solid Rocket Booster Retrieval operations are discussed in detail. The recovery of expended boosters and associated hardware without damage attributable to retrieval procedures is the main goal. The retrieval force consists tof ship's personnel and retrieval team members, each of whom has been trained and is highly skilled in multi-faceted operations. The retrieval force is equipped with two specially-built, highly maneuverable ships outfitted with parachute reels, retrieval cranes, towing winches, large volume-low pressure air compressors, SCUBA diving gear, inflatable boats with outboard motors and diver-operated SRB dewatering devices. The two ships are deployed in sufficient time to conduct an electronic and visual search of the impact area prior to launch. Upon search completeion, each ship takes station a safe distance from the predetermined impact point initiating both visual and electronic search in the direction of flight path, ensuring SRB acquisition at splashdown. When safe, the ships enter the impact area and commence recovery of all floating flight hardware which is subsequently returned to the Disassembly Facility for refurbishment and reuse. Retrieval techniques have evolved in parallel with equipment and flight hardware configuration changes. Additional changes have been initiated to improve personnel safety.

  6. Automatic information retrieval of Chinese business card

    NASA Astrophysics Data System (ADS)

    Shi, GuangShun; Pan, Wumo; Jin, Jianming

    2003-01-01

    Document image understanding techniques have been widely used in many application domains. Various kinds of documents have been researched and different methods are developed for information retrieval purpose. In this paper we present a practical method to extract information items from Chinese business card. Before retrieval information in business card, the image of business card had been segmented into little text regions and each text region had been recognized. Because the typeset of business card is variable, and both English and Chinese characters are used, so there are errors in segmentation and recognition result. We focus on building a robust model that can tolerate errors and extract syntax pattern of each text lines in business card, which using both layout information and logical information. By this model, many errors will be identified and adjusted. Finally, correct property will be assigned to each text region in business card, and recognition errors will be corrected.

  7. PRIME: Phase Retrieval via Majorization-Minimization

    NASA Astrophysics Data System (ADS)

    Qiu, Tianyu; Babu, Prabhu; Palomar, Daniel P.

    2016-10-01

    This paper considers the phase retrieval problem in which measurements consist of only the magnitude of several linear measurements of the unknown, e.g., spectral components of a time sequence. We develop low-complexity algorithms with superior performance based on the majorization-minimization (MM) framework. The proposed algorithms are referred to as PRIME: Phase Retrieval vIa the Majorization-minimization techniquE. They are preferred to existing benchmark methods since at each iteration a simple surrogate problem is solved with a closed-form solution that monotonically decreases the original objective function. In total, four algorithms are proposed using different majorization-minimization techniques. Experimental results validate that our algorithms outperform existing methods in terms of successful recovery and mean square error under various settings.

  8. Regularization for Atmospheric Temperature Retrieval Problems

    NASA Technical Reports Server (NTRS)

    Velez-Reyes, Miguel; Galarza-Galarza, Ruben

    1997-01-01

    Passive remote sensing of the atmosphere is used to determine the atmospheric state. A radiometer measures microwave emissions from earth's atmosphere and surface. The radiance measured by the radiometer is proportional to the brightness temperature. This brightness temperature can be used to estimate atmospheric parameters such as temperature and water vapor content. These quantities are of primary importance for different applications in meteorology, oceanography, and geophysical sciences. Depending on the range in the electromagnetic spectrum being measured by the radiometer and the atmospheric quantities to be estimated, the retrieval or inverse problem of determining atmospheric parameters from brightness temperature might be linear or nonlinear. In most applications, the retrieval problem requires the inversion of a Fredholm integral equation of the first kind making this an ill-posed problem. The numerical solution of the retrieval problem requires the transformation of the continuous problem into a discrete problem. The ill-posedness of the continuous problem translates into ill-conditioning or ill-posedness of the discrete problem. Regularization methods are used to convert the ill-posed problem into a well-posed one. In this paper, we present some results of our work in applying different regularization techniques to atmospheric temperature retrievals using brightness temperatures measured with the SSM/T-1 sensor. Simulation results are presented which show the potential of these techniques to improve temperature retrievals. In particular, no statistical assumptions are needed and the algorithms were capable of correctly estimating the temperature profile corner at the tropopause independent of the initial guess.

  9. [Presence of Australia antigen in blood donors].

    PubMed

    Gota, F

    1980-01-01

    The differential diagnosis of type A and B viral hepatitis is discussed and guidelines for the prevention of post-transfusional hospital hepatitis are proposed. Methods for the immunological demonstration of HBs antigen are illustrated, together with the respective positivity percentages in blood donors.

  10. System For Retrieving Reusable Software

    NASA Technical Reports Server (NTRS)

    Van Warren, Lloyd; Beckman, Brian C.

    1993-01-01

    Encyclopedia of Software Components (ESC) is information-retrieval system of computer hardware and software providing access to generic reusable software tools and parts. Core of ESC is central tool base, which is repository of reusable software. It receives queries and submissions from user through local browser subsystem and receives authorized updates from maintenance subsystem. Sends retrievals to local browser subsystem and user's submissions to maintenance subsystem. Future versions will provide for advanced media, including voice and video, and will link system to database-management system. Programmers will not only retrieve software, but also modify, execute, and cross-link with other software.

  11. Mobile medical visual information retrieval.

    PubMed

    Depeursinge, Adrien; Duc, Samuel; Eggel, Ivan; Müller, Henning

    2012-01-01

    In this paper, we propose mobile access to peer-reviewed medical information based on textual search and content-based visual image retrieval. Web-based interfaces designed for limited screen space were developed to query via web services a medical information retrieval engine optimizing the amount of data to be transferred in wireless form. Visual and textual retrieval engines with state-of-the-art performance were integrated. Results obtained show a good usability of the software. Future use in clinical environments has the potential of increasing quality of patient care through bedside access to the medical literature in context.

  12. Montana StreamStats—A method for retrieving basin and streamflow characteristics in Montana: Chapter A in Montana StreamStats

    USGS Publications Warehouse

    McCarthy, Peter M.; Dutton, DeAnn M.; Sando, Steven K.; Sando, Roy

    2016-04-05

    The U.S. Geological Survey (USGS) provides streamflow characteristics and other related information needed by water-resource managers to protect people and property from floods, plan and manage water-resource activities, and protect water quality. Streamflow characteristics provided by the USGS, such as peak-flow and low-flow frequencies for streamflow-gaging stations, are frequently used by engineers, flood forecasters, land managers, biologists, and others to guide their everyday decisions. In addition to providing streamflow characteristics at streamflow-gaging stations, the USGS also develops regional regression equations and drainage area-adjustment methods for estimating streamflow characteristics at locations on ungaged streams. Regional regression equations can be complex and often require users to determine several basin characteristics, which are physical and climatic characteristics of the stream and its drainage basin. Obtaining these basin characteristics for streamflow-gaging stations and ungaged sites traditionally has been time consuming and subjective, and led to inconsistent results.StreamStats is a Web-based geographic information system application that was created by the USGS to provide users with access to an assortment of analytical tools that are useful for water-resource planning and management. StreamStats allows users to easily obtain streamflow and basin characteristics for USGS streamflow-gaging stations and user-selected locations on ungaged streams. The USGS, in cooperation with Montana Department of Transportation, Montana Department of Environmental Quality, and Montana Department of Natural Resources and Conservation, completed a study to develop a StreamStats application for Montana, compute streamflow characteristics at streamflow-gaging stations, and develop regional regression equations to estimate streamflow characteristics at ungaged sites. Chapter A of this Scientific Investigations Report describes the Montana Stream

  13. Mapping epitopes and antigenicity by site-directed masking

    PubMed Central

    Paus, Didrik; Winter, Greg

    2006-01-01

    Here we describe a method for mapping the binding of antibodies to the surface of a folded antigen. We first created a panel of mutant antigens (β-lactamase) in which single surface-exposed residues were mutated to cysteine. We then chemically tethered the cysteine residues to a solid phase, thereby masking a surface patch centered on each cysteine residue and blocking the binding of antibodies to this region of the surface. By these means we mapped the epitopes of several mAbs directed to β-lactamase. Furthermore, by depleting samples of polyclonal antisera to the masked antigens and measuring the binding of each depleted sample of antisera to unmasked antigen, we mapped the antigenicity of 23 different epitopes. After immunization of mice and rabbits with β-lactamase in Freund’s adjuvant, we found that the antisera reacted with both native and denatured antigen and that the antibody response was mainly directed to an exposed and flexible loop region of the native antigen. By contrast, after immunization in PBS, we found that the antisera reacted only weakly with denatured antigen and that the antibody response was more evenly distributed over the antigenic surface. We suggest that denatured antigen (created during emulsification in Freund’s adjuvant) elicits antibodies that bind mainly to the flexible regions of the native protein and that this explains the correlation between antigenicity and backbone flexibility. Denaturation of antigen during vaccination or natural infections would therefore be expected to focus the antibody response to the flexible loops. PMID:16754878

  14. T-cell responses to minor histocompatibility antigens.

    PubMed Central

    Lai, P K; Waterfield, J D; Gascoigne, N R; Sharrock, C E; Mitchison, N A

    1982-01-01

    We have investigated the helper and cytotoxic T-cell response to minor histocompatibility antigens and generated long term antigen-specific cell lines to them. Antigen-specific activity was selected for by regular restimulation with irradiated cells bearing the antigens in the presence of interleukin 2, so that alloreactivity to other cell surface antigens was gradually lost. Helper T cells cultured over several months were active in vivo and in vitro, but the culturing method eventually selected for cytotoxic T cells at the expense of helper T cells, with concomitant changes in the proportions of cells expressing the Lyt phenotypes. Individual long term cultures of cytotoxic T cells specific for minor histocompatibility antigens were restricted by either H2K or D but not both. Helper T cells to minor histocompatibility antigens derived directly from primed F1 mice did not show restriction to the priming parental haplotype. This is consistent with antigen reprocessing by the F1 antigen presenting cells such that populations of helper T cells restricted by both parental H-2 haplotypes were primed. F1 cytotoxic T cells were restricted to the parental H-2 haplotype used for in vitro boosting, irrespective of which H-2 was used for in vivo priming. PMID:6214502

  15. Retrieving the vertical distribution of chlorophyll a concentration and phytoplankton community composition from in situ fluorescence profiles: A method based on a neural network with potential for global-scale applications

    NASA Astrophysics Data System (ADS)

    Sauzède, R.; Claustre, H.; Jamet, C.; Uitz, J.; Ras, J.; Mignot, A.; D'Ortenzio, F.

    2015-01-01

    neural network-based method is developed to assess the vertical distribution of (1) chlorophyll a concentration ([Chl]) and (2) phytoplankton community size indices (i.e., microphytoplankton, nanophytoplankton, and picophytoplankton) from in situ vertical profiles of chlorophyll fluorescence. This method (FLAVOR for Fluorescence to Algal communities Vertical distribution in the Oceanic Realm) uses as input only the shape of the fluorescence profile associated with its acquisition date and geo-location. The neural network is trained and validated using a large database including 896 concomitant in situ vertical profiles of High-Performance Liquid Chromatography (HPLC) pigments and fluorescence. These profiles were collected during 22 oceanographic cruises representative of the global ocean in terms of trophic and oceanographic conditions, making our method applicable to most oceanic waters. FLAVOR is validated with respect to the retrieval of both [Chl] and phytoplankton size indices using an independent in situ data set and appears to be relatively robust spatially and temporally. To illustrate the potential of the method, we applied it to in situ measurements of the BATS (Bermuda Atlantic Time Series Study) site and produce monthly climatologies of [Chl] and associated phytoplankton size indices. The resulting climatologies appear very promising compared to climatologies based on available in situ HPLC data. With the increasing availability of spatially and temporally well-resolved data sets of chlorophyll fluorescence, one possible global-scale application of FLAVOR could be to develop 3-D and even 4-D climatologies of [Chl] and associated composition of phytoplankton communities. The Matlab and R codes of the proposed algorithm are provided as supporting information.

  16. Phase retrieval by coherent modulation imaging

    DOE PAGES

    Zhang, Fucai; Chen, Bo; Morrison, Graeme R.; ...

    2016-11-18

    Phase retrieval is a long-standing problem in imaging when only the intensity of the wavefield can be recorded. Coherent diffraction imaging (CDI) is a lensless technique that uses iterative algorithms to recover amplitude and phase contrast images from diffraction intensity data. For general samples, phase retrieval from a single diffraction pattern has been an algorithmic and experimental challenge. Here we report a method of phase retrieval that uses a known modulation of the sample exit-wave. This coherent modulation imaging (CMI) method removes inherent ambiguities of CDI and uses a reliable, rapidly converging iterative algorithm involving three planes. It works formore » extended samples, does not require tight support for convergence, and relaxes dynamic range requirements on the detector. CMI provides a robust method for imaging in materials and biological science, while its single-shot capability will benefit the investigation of dynamical processes with pulsed sources, such as X-ray free electron laser.« less

  17. Developments of aerosol retrieval algorithm for Geostationary Environmental Monitoring Spectrometer (GEMS) and the retrieval accuracy test

    NASA Astrophysics Data System (ADS)

    KIM, M.; Kim, J.; Jeong, U.; Ahn, C.; Bhartia, P. K.; Torres, O.

    2013-12-01

    A scanning UV-Visible spectrometer, the GEMS (Geostationary Environment Monitoring Spectrometer) onboard the GEO-KOMPSAT2B (Geostationary Korea Multi-Purpose Satellite) is planned to be launched in geostationary orbit in 2018. The GEMS employs hyper-spectral imaging with 0.6 nm resolution to observe solar backscatter radiation in the UV and Visible range. In the UV range, the low surface contribution to the backscattered radiation and strong interaction between aerosol absorption and molecular scattering can be advantageous in retrieving aerosol optical properties such as aerosol optical depth (AOD) and single scattering albedo (SSA). By taking the advantage, the OMI UV aerosol algorithm has provided information on the absorbing aerosol (Torres et al., 2007; Ahn et al., 2008). This study presents a UV-VIS algorithm to retrieve AOD and SSA from GEMS. The algorithm is based on the general inversion method, which uses pre-calculated look-up table with assumed aerosol properties and measurement condition. To obtain the retrieval accuracy, the error of the look-up table method occurred by the interpolation of pre-calculated radiances is estimated by using the reference dataset, and the uncertainties about aerosol type and height are evaluated. Also, the GEMS aerosol algorithm is tested with measured normalized radiance from OMI, a provisional data set for GEMS measurement, and the results are compared with the values from AERONET measurements over Asia. Additionally, the method for simultaneous retrieve of the AOD and aerosol height is discussed.

  18. 2d-retrieval For Mipas-envisat

    NASA Astrophysics Data System (ADS)

    Steck, T.; von Clarmann, T.; Grabowski, U.; Höpfner, M.

    Limb sounding of the Earth's atmosphere provides vertically high resolved profiles of geophysical parameters. The long ray path through the atmosphere makes limb sounders sensitive to even little abundant species. On the other hand, horizontal in- homogeneities, if not taken into account properly, can cause systematic errors within the retrieval process. Especially for limb emission measurements in the mid IR, at- mopheric temperature gradients result in considerable vmr retrieval errors if they are neglected. We present a dedicated method of taking full 2D fields of state parameters (indepen- dent of tangent points) into account in the forward model and in the retrieval. The basic idea is that the 2D state vector is updated sequentially for each limb scan. This method is applied to the 2D retrieval of temperature and vmr for simulated radiances as expected from MIPAS-ENVISAT.

  19. Retrieval of atmospheric properties with radiometric measurements using neural network

    NASA Astrophysics Data System (ADS)

    Chakraborty, Rohit; Maitra, Animesh

    2016-11-01

    Microwave radiometer is an effective instrument to monitor the atmosphere continuously in different weather conditions. It measures brightness temperatures at different frequency bands which are subjected to standard retrieval methods to obtain real time profiles of various atmospheric parameters such as temperature and humidity. But the retrieval techniques used by radiometer have to be adaptive to changing weather condition and location. In the present study, three retrieval techniques have been used to obtain the temperature and relative humidity profiles from brightness temperatures, namely; piecewise linear regression, feed forward neural network and neural back propagation network. The simulated results are compared with radiosonde observations using correlation analysis and error distribution. The analysis reveals that neural network with back propagation is the most accurate technique amongst the three retrieval methods utilized in this study.

  20. Diversification of visual media retrieval results using saliency detection

    NASA Astrophysics Data System (ADS)

    Muratov, Oleg; Boato, Giulia; De Natale, Franesco G. B.

    2013-03-01

    Diversification of retrieval results allows for better and faster search. Recently there has been proposed different methods for diversification of image retrieval results mainly utilizing text information and techniques imported from natural language processing domain. However, images contain visual information that is impossible to describe in text and the use of visual features is inevitable. Visual saliency is information about the main object of an image implicitly included by humans while creating visual content. For this reason it is naturally to exploit this information for the task of diversification of the content. In this work we study whether visual saliency can be used for the task of diversification and propose a method for re-ranking image retrieval results using saliency. The evaluation has shown that the use of saliency information results in higher diversity of retrieval results.

  1. Spaced retrieval treatment of anomia

    PubMed Central

    Fridriksson, Julius; Holland, Audrey L.; Beeson, Pélagie; Morrow, Leigh

    2006-01-01

    Background Spaced Retrieval (SR) is a treatment approach developed to facilitate recall of information by individuals with dementia. Essentially an errorless learning procedure that can be used to facilitate recall of a variety of information, SR gradually increases the interval between correct recall of target items. Aims Given the success of using SR in dementia, the purpose of this study was to explore its usefulness in improving naming by individuals with aphasia. The rate of acquisition and retention of items was compared between SR and a more traditional treatment technique—cueing hierarchy (CH). Also, each oral naming treatment was run concurrently with a single word writing treatment. Methods & Procedures Three participants who had moderate or severe naming impairments and agraphia were studied. Single-subject design was applied across oral and written naming and treated and untreated items. Outcomes & Results The results indicate that for these participants, SR resulted in improved naming of specific items. The data further suggest that SR compared favourably to CH with regard to both acquisition and retention of items. The participants also benefited nicely from the writing treatment. Conclusions These findings suggest SR may be an alternative for managing naming impairment resulting from aphasia. Furthermore, the study supports providing treatments aimed at two different modalities concurrently. PMID:16823467

  2. Convolutional neural network for pottery retrieval

    NASA Astrophysics Data System (ADS)

    Benhabiles, Halim; Tabia, Hedi

    2017-01-01

    The effectiveness of the convolutional neural network (CNN) has already been demonstrated in many challenging tasks of computer vision, such as image retrieval, action recognition, and object classification. This paper specifically exploits CNN to design local descriptors for content-based retrieval of complete or nearly complete three-dimensional (3-D) vessel replicas. Based on vector quantization, the designed descriptors are clustered to form a shape vocabulary. Then, each 3-D object is associated to a set of clusters (words) in that vocabulary. Finally, a weighted vector counting the occurrences of every word is computed. The reported experimental results on the 3-D pottery benchmark show the superior performance of the proposed method.

  3. Neural Mechanisms of Extinction Learning and Retrieval

    PubMed Central

    Quirk, Gregory J.; Mueller, Devin

    2009-01-01

    Emotional learning is necessary for individuals to survive and prosper. Once acquired, however, emotional associations are not always expressed. Indeed, the regulation of emotional expression under varying environmental conditions is essential for mental health. The simplest form of emotional regulation is extinction, in which conditioned responding to a stimulus decreases when the reinforcer is omitted. Two decades of research on the neural mechanisms of fear conditioning have laid the groundwork for understanding extinction. In this review, we summarize recent work on the neural mechanisms of extinction learning. Like other forms of learning, extinction occurs in three phases: acquisition, consolidation, and retrieval, each of which depends on specific structures (amygdala, prefrontal cortex, hippocampus), and molecular mechanisms (receptors and signaling pathways). Pharmacological methods to facilitate consolidation and retrieval of extinction, for both aversive and appetitive conditioning, are setting the stage for novel treatments for anxiety disorders and addictions. PMID:17882236

  4. Phase retrieval via propagation-based interferometry

    NASA Astrophysics Data System (ADS)

    Loetgering, L.; Froese, H.; Wilhein, T.; Rose, M.

    2017-03-01

    We present an extension of the Gerchberg-Saxton algorithm to allow for phase retrieval from interferometric data recorded at multiple sample-detector distances. A system of coupled waves is introduced where the information exchange can be controlled by use of relaxation parameters. Optimal parameters are investigated by numerical simulation and demonstrated to work with experimental data. We demonstrate that systematic errors such as pointing instabilities of the interfering waves involved and position uncertainties of the detector can be overcome by dimensional extension of the search space. Further it is shown that the proposed approach offers superior reconstruction quality as compared to conventional Gerchberg-Saxton type algorithms. We expect that the method described here will open up numerous possibilities for the correction of systematic errors in optical phase retrieval and lensless microscopy.

  5. Polar Embedding for Aurora Image Retrieval.

    PubMed

    Yang, Xi; Gao, Xinbo; Tian, Qi

    2015-11-01

    Exploring the multimedia techniques to assist scientists for their research is an interesting and meaningful topic. In this paper, we focus on the large-scale aurora image retrieval by leveraging the bag-of-visual words (BoVW) framework. To refine the unsuitable representation and improve the retrieval performance, the BoVW model is modified by embedding the polar information. The superiority of the proposed polar embedding method lies in two aspects. On the one hand, the polar meshing scheme is conducted to determine the interest points, which is more suitable for images captured by circular fisheye lens. Especially for the aurora image, the extracted polar scale-invariant feature transform (polar-SIFT) feature can also reflect the geomagnetic longitude and latitude, and thus facilitates the further data analysis. On the other hand, a binary polar deep local binary pattern (polar-DLBP) descriptor is proposed to enhance the discriminative power of visual words. Together with the 64-bit polar-SIFT code obtained via Hamming embedding, the multifeature index is performed to reduce the impact of false positive matches. Extensive experiments are conducted on the large-scale aurora image data set. The experimental result indicates that the proposed method improves the retrieval accuracy significantly with acceptable efficiency and memory cost. In addition, the effectiveness of the polar-SIFT scheme and polar-DLBP integration are separately demonstrated.

  6. Selective Convolutional Descriptor Aggregation for Fine-Grained Image Retrieval.

    PubMed

    Wei, Xiu-Shen; Luo, Jian-Hao; Wu, Jianxin; Zhou, Zhi-Hua

    2017-03-27

    Deep convolutional neural network models pretrained for the ImageNet classification task have been successfully adopted to tasks in other domains, such as texture description and object proposal generation, but these tasks require annotations for images in the new domain. In this paper, we focus on a novel and challenging task in the pure unsupervised setting: fine-grained image retrieval. Even with image labels, fine-grained images are difficult to classify, let alone the unsupervised retrieval task. We propose the Selective Convolutional Descriptor Aggregation (SCDA) method. SCDA firstly localizes the main object in fine-grained images, a step that discards the noisy background and keeps useful deep descriptors. The selected descriptors are then aggregated and dimensionality reduced into a short feature vector using the best practices we found. SCDA is unsupervised, using no image label or bounding box annotation. Experiments on six fine-grained datasets confirm the effectiveness of SCDA for fine-grained image retrieval. Besides, visualization of the SCDA features shows that they correspond to visual attributes (even subtle ones), which might explain SCDA's high mean average precision in fine-grained retrieval. Moreover, on general image retrieval datasets, SCDA achieves comparable retrieval results with state-of-the-art general image retrieval approaches.

  7. Learning Short Binary Codes for Large-scale Image Retrieval.

    PubMed

    Liu, Li; Yu, Mengyang; Shao, Ling

    2017-03-01

    Large-scale visual information retrieval has become an active research area in this big data era. Recently, hashing/binary coding algorithms prove to be effective for scalable retrieval applications. Most existing hashing methods require relatively long binary codes (i.e., over hundreds of bits, sometimes even thousands of bits) to achieve reasonable retrieval accuracies. However, for some realistic and unique applications, such as on wearable or mobile devices, only short binary codes can be used for efficient image retrieval due to the limitation of computational resources or bandwidth on these devices. In this paper, we propose a novel unsupervised hashing approach called min-cost ranking (MCR) specifically for learning powerful short binary codes (i.e., usually the code length shorter than 100 b) for scalable image retrieval tasks. By exploring the discriminative ability of each dimension of data, MCR can generate one bit binary code for each dimension and simultaneously rank the discriminative separability of each bit according to the proposed cost function. Only top-ranked bits with minimum cost-values are then selected and grouped together to compose the final salient binary codes. Extensive experimental results on large-scale retrieval demonstrate that MCR can achieve comparative performance as the state-of-the-art hashing algorithms but with significantly shorter codes, leading to much faster large-scale retrieval.

  8. An exponentiation method for XML element retrieval.

    PubMed

    Wichaiwong, Tanakorn

    2014-01-01

    XML document is now widely used for modelling and storing structured documents. The structure is very rich and carries important information about contents and their relationships, for example, e-Commerce. XML data-centric collections require query terms allowing users to specify constraints on the document structure; mapping structure queries and assigning the weight are significant for the set of possibly relevant documents with respect to structural conditions. In this paper, we present an extension to the MEXIR search system that supports the combination of structural and content queries in the form of content-and-structure queries, which we call the Exponentiation function. It has been shown the structural information improve the effectiveness of the search system up to 52.60% over the baseline BM25 at MAP.

  9. Immune recognition of protein antigens

    SciTech Connect

    Laver, W.G.; Air, G.M.

    1985-01-01

    This book contains 33 papers. Some of the titles are: Antigenic Structure of Influenze Virus Hemagglutinin; Germ-line and Somatic Diversity in the Antibody Response to the Influenza Virus A/PR/8/34 Hemagglutinin; Recognition of Cloned Influenza A Virus Gene Products by Cytotoxic T Lymphocytes; Antigenic Structure of the Influenza Virus N2 Neuraminidase; and The Molecular and Genetic Basis of Antigenic Variation in Gonococcal Pillin.

  10. A Scene Text-Based Image Retrieval System

    DTIC Science & Technology

    2012-12-01

    problems need to be solved. Most of the previous studies in text detection can be classified into approaches based on edge , connected component, and...order to detect and to merge edges from letters in images [2][3]. Edge based methods is fast and can have a high recall. However, it often produces...algorithms can be used in image retrieval applications. Keywords— text detection , text binarization, scene text, image retrieval, image indexing I

  11. Phase Retrieval with Signal Bias. Section 7.3

    NASA Technical Reports Server (NTRS)

    Thurman, Samuel T.; Fienup, James R.

    2009-01-01

    The effect of a uniform measurement bias, due to background light, stray light, detector dark current, or detector offset, on phase retrieval wavefront sensing algorithms is analyzed. Simulation results indicate that the root-mean-square error of the retrieved phase can be more sensitive to an unaccounted-for signal bias than to random noise in practical scenarios. Three methods for reducing the impact of signal bias are presented

  12. About Phase: Synthetic Aperture Radar and the Phase Retrieval

    DTIC Science & Technology

    2014-03-01

    techniques also enables detection of sudden seismic activity and even volcanic bulging prior to the eruption of volcanoes [2, 48, 71]. Navigation and...in SAR could have a more systematic solution if only additional signal data were available for analysis. The desire for more information motivates...transform. This reduction implies that any method of phase retrieval is also a solution to the phase error problem. We note that phase retrieval is

  13. Visualizing and improving the robustness of phase retrieval algorithms

    DOE PAGES

    Tripathi, Ashish; Leyffer, Sven; Munson, Todd; ...

    2015-06-01

    Coherent x-ray diffractive imaging is a novel imaging technique that utilizes phase retrieval and nonlinear optimization methods to image matter at nanometer scales. We explore how the convergence properties of a popular phase retrieval algorithm, Fienup's HIO, behave by introducing a reduced dimensionality problem allowing us to visualize and quantify convergence to local minima and the globally optimal solution. We then introduce generalizations of HIO that improve upon the original algorithm's ability to converge to the globally optimal solution.

  14. The use of ammonium sulphate precipitation method for the determination of antigen-binding capacity and affinity of anti-tetanus antibodies in human serum.

    PubMed

    Alausa, O K

    1975-01-01

    Radioimmunoassay of anti-tetanus antibodies produced in human serum in response to tetanus toxoid immunization was carried out by employing the non-specific precipitation effect of ammonium sulphate described by Farr (1958). The results showed that the method was sensitive and was able to differentiate between immune and non-immune persons. The effects of booster dose injection on the quantity and quality of antibodies produced during immunization were discussed, and the possible use of the method to predict the amount of immunogen, the timing and number of injections required for optimal host response in immunization schedules was suggested.

  15. Elaborative Retrieval: Do Semantic Mediators Improve Memory?

    ERIC Educational Resources Information Center

    Lehman, Melissa; Karpicke, Jeffrey D.

    2016-01-01

    The elaborative retrieval account of retrieval-based learning proposes that retrieval enhances retention because the retrieval process produces the generation of semantic mediators that link cues to target information. We tested 2 assumptions that form the basis of this account: that semantic mediators are more likely to be generated during…

  16. O-antigen and virulence profiling of Shiga toxin-producing Escherichia coli by a rapid and cost-effective DNA microarray colorimetric method

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Shiga toxin-producing Escherichia coli (STEC) is a leading cause of foodborne illness worldwide. To evaluate better methods to rapidly detect and genotype Shiga toxin-producing Escherichia coli strains, the present study evaluated the use of the ampliPHOX colorimetric detection technology, based on ...

  17. Cloud Property Retrieval Products for Graciosa Island, Azores

    DOE Data Explorer

    Dong, Xiquan

    2014-05-05

    The motivation for developing this product was to use the Dong et al. 1998 method to retrieve cloud microphysical properties, such as cloud droplet effective radius, cloud droplets number concentration, and optical thickness. These retrieved properties have been used to validate the satellite retrieval, and evaluate the climate simulations and reanalyses. We had been using this method to retrieve cloud microphysical properties over ARM SGP and NSA sites. We also modified the method for the AMF at Shouxian, China and some IOPs, e.g. ARM IOP at SGP in March, 2000. The ARSCL data from ARM data archive over the SGP and NSA have been used to determine the cloud boundary and cloud phase. For these ARM permanent sites, the ARSCL data was developed based on MMCR measurements, however, there were no data available at the Azores field campaign. We followed the steps to generate this derived product and also include the MPLCMASK cloud retrievals to determine the most accurate cloud boundaries, including the thin cirrus clouds that WACR may under-detect. We use these as input to retrieve the cloud microphysical properties. Due to the different temporal resolutions of the derived cloud boundary heights product and the cloud properties product, we submit them as two separate netcdf files.

  18. Antigenic cartographic analysis of H7 avian influenza viruses with chicken serum

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Antigenic cartography is a relatively new method that can be used to evaluate the antigenic relatedness among avian influenza virus isolates. Evaluation of antigenic relationships among avian influenza viruses can be applied to vaccine design and to understanding the evolution of the virus. Initia...

  19. Use of antigenic cartography in vaccine seed strain selection.

    PubMed

    Fouchier, Ron A M; Smith, Derek J

    2010-03-01

    Human influenza A viruses are classic examples of antigenically variable pathogens that have a seemingly endless capacity to evade the host's immune response. The viral hemagglutinin (HA) and neuraminidase (NA) proteins are the main targets of our antibody response to combat infections. HA and NA continuously change to escape from humoral immunity, a process known as antigenic drift. As a result of antigenic drift, the human influenza vaccine is updated frequently. The World Health Organization (WHO) coordinates a global influenza surveillance network that, by the hemagglutination inhibition (HI) assay, routinely characterizes the antigenic properties of circulating strains in order to select new seed viruses for such vaccine updates. To facilitate a quantitative interpretation and easy visualization of HI data, a new computational technique called "antigenic cartography" was developed. Since its development, antigenic cartography has been applied routinely to assist the WHO with influenza surveillance activities. Until recently, antigenic variation was not considered a serious issue with influenza vaccines for poultry. However, because of the diversification of the Asian H5N1 lineage since 1996 into multiple genetic clades and subclades, and because of the long-term use of poultry vaccines against H5 in some parts of the world, this issue needs to be re-addressed. The antigenic properties of panels of avian H5N1 viruses were characterized by HI assay, using mammalian or avian antisera, and analyzed using antigenic cartography methods. These analyses revealed antigenic differences between circulating H5N1 viruses and the H5 viruses used in poultry vaccines. Considerable antigenic variation was also observed within and between H5N1 clades. These observations have important implications for the efficacy and long-term use of poultry vaccines.

  20. Collaborative study on antigens for immunodiagnosis of schistosomiasis*

    PubMed Central

    Mott, K. E.; Dixon, H.

    1982-01-01

    Eight research laboratories in Europe and the United States of America were selected on the basis of having published data on Schistosoma mansoni and S. japonicum antigens to participate in a study of various antigen/test combinations for immunodiagnosis of schistosomiasis. The serum bank consisted of 395 well documented sera from four endemic areas in Brazil (2 areas), Kenya, and the Philippines. Altogether, 21 S. mansoni and four S. japonicum antigen and immunoassay combinations were evaluated. S. mansoni egg antigens yielded a higher combined sensitivity than adult worm antigens, irrespective of their purity, in active S. mansoni infections before and after specific treatment. Quantitative seroreactivity of characterized S. mansoni egg antigens showed good correlation with faecal egg counts in the 5-14 year age group. No correlation between morbidity related to S. mansoni and seroreactivity was observed in any test system. Three S. japonicum egg antigens showed high sensitivity and specificity in relation to the presence or absence of eggs in the stool. The quantitative seroreactivity of the characterized S. japonicum egg antigens correlated directly with the intensity of S. japonicum infection in all age groups. The enzyme-linked immunosorbent assay (ELISA), using several different procedures, performed well with the antigens used in the study. The indium slide immunoassay (ISI), a simple qualitative visual test system using an S. mansoni egg antigen, demonstrated a high degree of sensitivity and specificity. The results did not indicate the superiority of any particular immunodiagnostic method for detecting antischistosome antibodies. This collaborative study is considered a first step towards developing and standardizing antigens for immunodiagnosis of schistosomiasis. PMID:6983926

  1. The role of retrieval mode and retrieval orientation in retrieval practice: insights from comparing recognition memory testing formats and restudying.

    PubMed

    Gao, Chuanji; Rosburg, Timm; Hou, Mingzhu; Li, Bingbing; Xiao, Xin; Guo, Chunyan

    2016-12-01

    The effectiveness of retrieval practice for aiding long-term memory, referred to as the testing effect, has been widely demonstrated. However, the specific neurocognitive mechanisms underlying this phenomenon remain unclear. In the present study, we sought to explore the role of pre-retrieval processes at initial testing on later recognition performance by using event-related potentials (ERPs). Subjects studied two lists of words (Chinese characters) and then performed a recognition task or a source memory task, or restudied the word lists. At the end of the experiment, subjects received a final recognition test based on the remember-know paradigm. Behaviorally, initial testing (active retrieval) enhanced memory retention relative to restudying (passive retrieval). The retrieval mode at initial testing was indexed by more positive-going ERPs for unstudied items in the active-retrieval tasks than in passive retrieval from 300 to 900 ms. Follow-up analyses showed that the magnitude of the early ERP retrieval mode effect (300-500 ms) was predictive of the behavioral testing effect later on. In addition, the ERPs for correctly rejected new items during initial testing differed between the two active-retrieval tasks from 500 to 900 ms, and this ERP retrieval orientation effect predicted differential behavioral testing gains between the two active-retrieval conditions. Our findings confirm that initial testing promotes later retrieval relative to restudying, and they further suggest that adopting pre-retrieval processing in the forms of retrieval mode and retrieval orientation might contribute to these memory enhancements.

  2. Comparison of texture models for efficient ultrasound image retrieval

    NASA Astrophysics Data System (ADS)

    Bansal, Maggi; Sharma, Vipul; Singh, Sukhwinder

    2013-02-01

    Due to availability of inexpensive and easily available image capturing devices, the size of digital image collection is increasing rapidly. Thus, there is need to create efficient access methods or retrieval tools to search, browse and retrieve images from large multimedia repositories. More specifically, researchers have been engaged on different ways of retrieving images based on their actual content. In particular, Content Based Image Retrieval (CBIR) systems have attracted considerable research and commercial interest in the recent years. In CBIR, visual features characterizing the image content are color, shape and texture. Currently, texture is used to quantify the image content of medical images as it is the most prominent feature that contains information about the spatial distribution of gray levels and variations in brightness. Various texture models like Haralick's Spatial Gray Level Co-occurence Matrix (SGLCM), Gray Level Difference Statistics (GLDS), First-order Statistics (FoS), Statistical Feature Matrix (SFM), Law's Texture Energy Measures (TEM), Fractal features and Fourier Power Spectrum (FPS) features exists in literature. Each of these models visualizes texture in a different way. Retrieval performance depends upon the choice of texture algorithm. Unfortunately, there is no texture model known to work best for encoding texture properties of liver ultrasound images or retrieving most similar images. An experimental comparison of different texture models for Content Based Medical Image Retrieval (CBMIR) is presented in this paper. For the experiments, liver ultrasound image database is used and the retrieval performance of the various texture models is analyzed in detail. The paper concludes with recommendations which texture model performs better for liver ultrasound images. Interestingly, FPS and SGLCM based Haralick's features perform well for liver ultrasound retrieval and thus can be recommended as a simple baseline for such images.

  3. Radiative Transfer and Retrievals in EOF Domain

    NASA Technical Reports Server (NTRS)

    Liu, Xu; Zhou, Daniel K.; Larar, Allen; Smith, William L.; Schluessel, Peter

    2008-01-01

    The Infrared Atmospheric Sounding Interferometer (IASI) is a hyperspectral sensor with 8461 spectral channels and a nominal spectral resolution of 0.25 cm(sup -1). It is computationally intensive to perform radiative transfer calculations and inversions using all these channels. We will present a Principal Component-based Radiative Transfer Model (PCRTM) and a retrieval algorithm which perform all the necessary calculations in EOF domain. Since the EOFs are orthogonal to each other, only about 100 principal components are needed to represent the information content of the 8461 channels. The PCRTM provides the EOF coefficients and associated derivatives with respect to atmospheric and surface parameters needed by the inversion algorithm. The inversion algorithm is based on a non-linear Levenberg-Marquardt method with climatology covariance and a priori information as constraints. The retrieved parameters include atmospheric temperature, moisture and ozone profiles, cloud parameters, surface skin temperature, and surface emissivities. To make the retrieval system even more compact and stable. The atmospheric vertical profiles are compressed into the EOF space as well. The surface emissivities are also compressed into EOF space.

  4. The Application of ElasticSearch in the Massive Astronomical Data Retrieval

    NASA Astrophysics Data System (ADS)

    Chen, Y. J.; Wang, F.; Deng, H.; Liu, Y. B.

    2016-03-01

    Astronomical observational data are the fundamental element for modern astronomical researches. However, with the rapid increase of astronomical data, the traditional centralized retrieval methods are hard to meet the requirements of high- performance data retrieval. In the study, we present a novel method which is based on the ElasticSearch distributed retrieval engine and River mechanism to create data indexes, and provide high performance data retrieval for massive FITS (Flexible Image Transport System) data. We discuss the key technologies of the nearly real-time retrieval and query. The experimental results show that the method is capable of obtaining high retrieval performance especially for the cases in which the number of the FITS data exceeds millions or even tens of millions. Meanwhile, the method can be easily integrated into the current astronomical data archiving systems, and completely meet the archive requirements of all kinds of astronomical telescope systems.

  5. Comparison of Binax Legionella Urinary Antigen EIA kit with Binax RIA Urinary Antigen kit for detection of Legionella pneumophila serogroup 1 antigen.

    PubMed Central

    Hackman, B A; Plouffe, J F; Benson, R F; Fields, B S; Breiman, R F

    1996-01-01

    The Legionella Urinary Antigen EIA kit (Binax, Portland, Maine) was compared with the EQUATE RIA Legionella Urinary Antigen kit (Binax) for its ability to detect the presence of urinary antigens to Legionella pneumophila serogroup 1. Urine specimens from patients without Legionnaires' disease (n = 33) were negative by both methods (specificity, 100%). Twenty (77%) of 26 urine specimens from patients with Legionnaires' disease positive by the radioimmunoassay kit were also positive by the enzyme immunoassay (EIA) kit. If the cutoff for a positive EIA result were lowered to a ration of > or = 2.5, 23 of 26 (88%) urine specimens would have been positive by EIA and the specificity would remain 100%. Use of the EIA kit is an acceptable method for detecting L. pneumophila serogroup 1 urinary antigens by laboratories that do not want to handle radioactive materials. PMID:8735125

  6. An improved glyoxal retrieval from OMI measurements

    NASA Astrophysics Data System (ADS)

    Alvarado, L. M. A.; Richter, A.; Vrekoussis, M.; Wittrock, F.; Hilboll, A.; Schreier, S. F.; Burrows, J. P.

    2014-12-01

    Satellite observations from the SCIAMACHY, GOME-2 and OMI spectrometers have been used to retrieve atmospheric columns of glyoxal (CHOCHO) with the DOAS method. High CHOCHO levels were found over regions with large biogenic and pyrogenic emissions, and hot-spots have been identified over areas of anthropogenic activities. This study focuses on the development of an improved retrieval for CHOCHO from measurements by the OMI instrument. From sensitivity tests, a fitting window and a polynomial degree are determined. Two different approaches to reduce the interference of liquid water absorption over oceanic regions are evaluated, achieving significant reduction of the number of negative columns over clear water regions. The impact of using different absorption cross-sections for water vapour is evaluated and only small differences are found. Finally, a high-temperature (boundary layer ambient: 294 K) absorption cross-section of nitrogen dioxide (NO2) is introduced in the DOAS retrieval to account for potential interferences of NO2 over regions with large anthropogenic emissions, leading to improved fit quality over these areas. A comparison with vertical CHOCHO columns retrieved from GOME-2 and SCIAMACHY measurements over continental regions is performed, showing overall good consistency. However, SCIAMACHY CHOCHO columns are systematically higher than those obtained from the other instruments. Using the new OMI CHOCHO data set, the link between fires and glyoxal columns is investigated for two selected regions in Africa. In addition, mapped averages are computed for a fire event in Russia between mid-July and mid-August 2010. In both cases, enhanced CHOCHO levels are found in close spatial and temporal proximity to elevated levels of MODIS fire radiative power, demonstrating that pyrogenic emissions can be clearly identified in the new OMI CHOCHO product.

  7. Novel antigen delivery systems

    PubMed Central

    Trovato, Maria; Berardinis, Piergiuseppe De

    2015-01-01

    Vaccines represent the most relevant contribution of immunology to human health. However, despite the remarkable success achieved in the past years, many vaccines are still missing in order to fight important human pathologies and to prevent emerging and re-emerging diseases. For these pathogens the known strategies for making vaccines have been unsuccessful and thus, new avenues should be investigated to overcome the failure of clinical trials and other important issues including safety concerns related to live vaccines or viral vectors, the weak immunogenicity of subunit vaccines and side effects associated with the use of adjuvants. A major hurdle of developing successful and effective vaccines is to design antigen delivery systems in such a way that optimizes antigen presentation and induces broad protective immune responses. Recent advances in vector delivery technologies, immunology, vaccinology and system biology, have led to a deeper understanding of the molecular and cellular mechanisms by which vaccines should stimulate both arms of the adaptive immune responses, offering new strategies of vaccinations. This review is an update of current strategies with respect to live attenuated and inactivated vaccines, DNA vaccines, viral vectors, lipid-based carrier systems such as liposomes and virosomes as well as polymeric nanoparticle vaccines and virus-like particles. In addition, this article will describe our work on a versatile and immunogenic delivery system which we have studied in the past decade and which is derived from a non-pathogenic prokaryotic organism: the “E2 scaffold” of the pyruvate dehydrogenase complex from Geobacillus stearothermophilus. PMID:26279977

  8. MODIS cloud and aerosol retrieval simulator and its applications

    NASA Astrophysics Data System (ADS)

    Wind, Galina

    Executing a cloud or aerosol physical properties retrieval algorithm from controlled synthetic data is an important step in retrieval algorithm development. Synthetic data can help answer questions about the sensitivity and performance of the algorithm or aid in determining how an existing retrieval algorithm may perform with a planned sensor. Synthetic data can also help in solving issues that may have surfaced in the retrieval results. Synthetic data become very important when other validation methods, such as field campaigns,are of limited scope. These tend to be of relatively short duration and often are costly. Ground stations have limited spatial coverage whilesynthetic data can cover large spatial and temporal scales and a wide variety of conditions at a low cost. In this work I develop an advanced cloud and aerosol retrieval simulator for the MODIS instrument, also known as Multi-sensor Cloud and Aerosol Retrieval Simulator (MCARS). In a close collaboration with the modeling community I have seamlessly combined the GEOS-5 global climate model with the DISORT radiative transfer code, widely used by the remote sensing community, with the observations from the MODIS instrument to create the simulator. With the MCARS simulator it was then possible to solve the long standing issue with the MODIS aerosol optical depth retrievals that had a low bias for smoke aerosols. MODIS aerosol retrieval did not account for effects of humidity on smoke aerosols. The MCARS simulator also revealed an issue that has not been recognized previously, namely,the value of fine mode fraction could create a linear dependence between retrieved aerosol optical depth and land surface reflectance. MCARS provided the ability to examine aerosol retrievals against "ground truth" for hundreds of thousands of simultaneous samples for an area covered by only three AERONET ground stations. Findings from MCARS are already being used to improve the performance of operational MODIS aerosol

  9. An update on sperm retrieval techniques for azoospermic males

    PubMed Central

    Esteves, Sandro C; Miyaoka, Ricardo; Orosz, José Eduardo; Agarwal, Ashok

    2013-01-01

    The use of non-ejaculated sperm coupled with intracytoplasmic sperm injection has become a globally established procedure for couples with azoospermic male partners who wish to have biological offspring. Surgical methods have been developed to retrieve spermatozoa from the epididymides and the testes of such patients. This article reviews the methods currently available for sperm acquisition in azoospermia, with a particular focus on the perioperative, anesthetic and technical aspects of these procedures. A critical analysis of the advantages and disadvantages of these sperm retrieval methods is provided, including the authors' methods of choice and anesthesia preferences. PMID:23503959

  10. Stool Test: H. Pylori Antigen

    MedlinePlus

    ... Your 1- to 2-Year-Old Stool Test: H. Pylori Antigen KidsHealth > For Parents > Stool Test: H. Pylori Antigen A A A What's in this article? ... en español Muestra de materia fecal: antígeno de H. pylori What It Is Helicobacter pylori ( H. pylori ) bacteria ...

  11. Development of Prototype Filovirus Recombinant Antigen Immunoassays

    PubMed Central

    Boisen, Matt L.; Oottamasathien, Darin; Jones, Abigail B.; Millett, Molly M.; Nelson, Diana S.; Bornholdt, Zachary A.; Fusco, Marnie L.; Abelson, Dafna M.; Oda, Shun-ichiro; Hartnett, Jessica N.; Rowland, Megan M.; Heinrich, Megan L.; Akdag, Marjan; Goba, Augustine; Momoh, Mambu; Fullah, Mohammed; Baimba, Francis; Gbakie, Michael; Safa, Sadiki; Fonnie, Richard; Kanneh, Lansana; Cross, Robert W.; Geisbert, Joan B.; Geisbert, Thomas W.; Kulakosky, Peter C.; Grant, Donald S.; Shaffer, Jeffery G.; Schieffelin, John S.; Wilson, Russell B.; Saphire, Erica Ollmann; Branco, Luis M.; Garry, Robert F.; Khan, S. Humarr; Pitts, Kelly R.

    2015-01-01

    Background. Throughout the 2014–2015 Ebola outbreak in West Africa, major gaps were exposed in the availability of validated rapid diagnostic platforms, protective vaccines, and effective therapeutic agents. These gaps potentiated the development of prototype rapid lateral flow immunodiagnostic (LFI) assays that are true point-of-contact platforms, for the detection of active Ebola infections in small blood samples. Methods. Recombinant Ebola and Marburg virus matrix VP40 and glycoprotein (GP) antigens were used to derive a panel of monoclonal and polyclonal antibodies. Antibodies were tested using a multivariate approach to identify antibody-antigen combinations suitable for enzyme-linked immunosorbent assay (ELISA) and LFI assay development. Results. Polyclonal antibodies generated in goats were superior reagents for capture and detection of recombinant VP40 in test sample matrices. These antibodies were optimized for use in antigen-capture ELISA and LFI assay platforms. Prototype immunoglobulin M (IgM)/immunoglobulin G (IgG) ELISAs were similarly developed that specifically detect Ebola virus–specific antibodies in the serum of experimentally infected nonhuman primates and in blood samples obtained from patients with Ebola from Sierra Leone. Conclusions. The prototype recombinant Ebola LFI assays developed in these studies have sensitivities that are useful for clinical diagnosis of acute ebolavirus infections. The antigen-capture and IgM/IgG ELISAs provide additional confirmatory assay platforms for detecting VP40 and other ebolavirus-specific immunoglobulins. PMID:26232440

  12. A Probabilistic Analysis of Sparse Coded Feature Pooling and Its Application for Image Retrieval

    PubMed Central

    Zhang, Yunchao; Chen, Jing; Huang, Xiujie; Wang, Yongtian

    2015-01-01

    Feature coding and pooling as a key component of image retrieval have been widely studied over the past several years. Recently sparse coding with max-pooling is regarded as the state-of-the-art for image classification. However there is no comprehensive study concerning the application of sparse coding for image retrieval. In this paper, we first analyze the effects of different sampling strategies for image retrieval, then we discuss feature pooling strategies on image retrieval performance with a probabilistic explanation in the context of sparse coding framework, and propose a modified sum pooling procedure which can improve the retrieval accuracy significantly. Further we apply sparse coding method to aggregate multiple types of features for large-scale image retrieval. Extensive experiments on commonly-used evaluation datasets demonstrate that our final compact image representation improves the retrieval accuracy significantly. PMID:26132080

  13. Do FY antigens act as minor histocompatibility antigens in the graft-versus-host disease paradigm after human leukocyte antigen-identical sibling hematopoietic stem cell transplantation?

    PubMed

    Sellami, Mohamed Hichem; Chaabane, Manel; Kaabi, Houda; Torjemane, Lamia; Ladeb, Saloua; Ben Othmane, Tarek; Hmida, Slama

    2012-03-01

    FY antigens are candidate minor histocompatibility antigens relevant to renal allograft rejection, but no data have been reported about their role in graft-versus-host disease (GVHD) incidence after human leukocyte antigen (HLA)-identical siblings hematopoietic stem cell transplantation (HSCT). The aim of this study was to examine the effect of donor/recipient disparity at FY antigens on the incidence of GVHD in Tunisian patients receiving an HLA-identical HSCT. This work enrolled 105 Tunisian pairs of recipients and their HLA-identical sibling donors of HSCs. FY genotyping was performed with the polymerase chain reaction-sequence-specific primer method and donor/recipient disparity for these antigens was analyzed at two levels: incompatibility and nonidentity. The case-control analyses showed no significant correlation between FY disparity and the incidence of either acute or chronic GVHD. Sample size calculation showed that 572 cases and 1716 controls would be necessary to be able to detect a significant association with 80% power and two-sided type I error level of 5% (α=0.05). The lack of association in the studied cohort may be explained by the low immunogenicity of FY antigens in HSCT context, compared with other antigens such as HA-1 and CD31.

  14. Intelligent web image retrieval system

    NASA Astrophysics Data System (ADS)

    Hong, Sungyong; Lee, Chungwoo; Nah, Yunmook

    2001-07-01

    Recently, the web sites such as e-business sites and shopping mall sites deal with lots of image information. To find a specific image from these image sources, we usually use web search engines or image database engines which rely on keyword only retrievals or color based retrievals with limited search capabilities. This paper presents an intelligent web image retrieval system. We propose the system architecture, the texture and color based image classification and indexing techniques, and representation schemes of user usage patterns. The query can be given by providing keywords, by selecting one or more sample texture patterns, by assigning color values within positional color blocks, or by combining some or all of these factors. The system keeps track of user's preferences by generating user query logs and automatically add more search information to subsequent user queries. To show the usefulness of the proposed system, some experimental results showing recall and precision are also explained.

  15. Retrieval interference in sentence comprehension

    PubMed Central

    Van Dyke, Julie A.; McElree, Brian

    2007-01-01

    The role of interference effects in sentence processing has recently begun to receive attention, however whether these effects arise during encoding or retrieval remains unclear. This paper draws on basic memory research to help distinguish these explanations and reports data from an experiment that manipulates the possibility for retrieval interference while holding encoding conditions constant. We found clear support for the principle of cue-overload, wherein cues available at retrieval cannot uniquely distinguish among competitors, thus giving rise to interference effects. We discuss the data in relation to a cue-based parsing framework (Van Dyke & Lewis, 2003) and other interference effects observed in sentence processing (e.g., Gordon, Hendrick, & Johnson, 2001, 2004). We conclude from the available data that the memory system that subserves language comprehension operates according to similar principles as memory in other domains. PMID:18209744

  16. Radioimmunoassays of hidden viral antigens

    SciTech Connect

    Neurath, A.R.; Strick, N.; Baker, L.; Krugman, S.

    1982-07-01

    Antigens corresponding to infectious agents may be present in biological specimens only in a cryptic form bound to antibodies and, thus, may elude detection. We describe a solid-phase technique for separation of antigens from antibodies. Immune complexes are precipitated from serum by polyethylene glycol, dissociated with NaSCN, and adsorbed onto nitrocellulose or polystyrene supports. Antigens remain topographically separated from antibodies after removal of NaSCN and can be detected with radiolabeled antibodies. Genomes from viruses immobilized on nitrocellulose can be identified by nucleic acid hybridization. Nanogram quantities of sequestered hepatitis B surface and core antigens and picogram amounts of hepatitis B virus DNA were detected. Antibody-bound adenovirus, herpesvirus, and measles virus antigens were discerned by the procedure.

  17. Radioimmunoassays of hidden viral antigens.

    PubMed Central

    Neurath, A R; Strick, N; Baker, L; Krugman, S

    1982-01-01

    Antigens corresponding to infectious agents may be present in biological specimens only in a cryptic form bound to antibodies and, thus, may elude detection. We describe a solid phase technique for separation of antigens from antibodies. Immune complexes are precipitated from serum by polyethylene glycol, dissociated with NaSCN, and adsorbed onto nitrocellulose or polystyrene supports. Antigens remain topographically separated from antibodies after removal of NaSCN and can be detected with radiolabeled antibodies. Genomes from viruses immobilized on nitrocellulose can be identified by nucleic acid hybridization. Nanogram quantities of sequestered hepatitis B surface and core antigens and picogram amounts of hepatitis B virus DNA were detected. Antibody-bond adenovirus, herpesvirus, and measles virus antigens were discerned by the procedure. Images PMID:6956871

  18. SNUMedinfo at TREC CDS Track 2014: Medical Case-based Retrieval Task

    DTIC Science & Technology

    2014-11-01

    at the TREC Clinical Decision Support track 2014. This task is about medical case- based retrieval. Case description is used as query text . Per each...knowledge-based query expan- sion method for the relevance ranking. Secondly, machine learning classifier based text categorization method is used for the...performance improvements. Keywords: Case-based retrieval, Query expansion, Text categorization, Infor- mation retrieval 1. Introduction In this

  19. Carbohydrate-Mediated Targeting of Antigen to Dendritic Cells Leads to Enhanced Presentation of Antigen to T Cells

    PubMed Central

    Adams, Eddie W.; Ratner, Daniel M.; Seeberger, Peter H.; Hacohen, Nir

    2009-01-01

    The unique therapeutic value of dendritic cells (DCs) for the treatment of allergy, autoimmunity and transplant rejection is predicated upon our ability to selectively deliver antigens, drugs or nucleic acids to DCs in vivo. Here we describe a method for delivering whole protein antigens to DCs based on carbohydrate-mediated targeting of DC-expressed lectins. A series of synthetic carbohydrates was chemically-coupled to a model antigen, ovalbumin (OVA), and each conjugate was evaluated for its ability to increase the efficiency of antigen presentation by murine DCs to OVA-specific T cells (CD4+ and CD8+). In vitro data are presented that demonstrate that carbohydrate modification of OVA leads to a 50-fold enhancement of presentation of antigenic peptide to CD4+ T cells. A tenfold enhancement is observed for CD8+ T cells; this indicates that the targeted lectin(s) can mediate cross-presentation of antigens on MHC class I. Our data indicate that the observed enhancements in antigen presentation are unique to OVA that is conjugated to complex oligosaccharides, such as a high-mannose nonasaccharide, but not to monosaccharides. Taken together, our data suggest that a DC targeting strategy that is based upon carbohydrate-lectin interactions is a promising approach for enhancing antigen presentation via class I and class II molecules. PMID:18186095

  20. Retrieval of atmospheric methane from high spectral resolution satellite measurements: a correction for cirrus cloud effects.

    PubMed

    Bril, Andrey; Oshchepkov, Sergey; Yokota, Tatsuya

    2009-04-10

    We assessed the accuracy of methane (CH(4)) retrievals from synthetic radiance spectra particular to Greenhouse Gases Observing Satellite observations. We focused on estimating the CH(4) vertical column amount from an atmosphere that includes thin cirrus clouds, taking into account uncertain meteorological conditions. A photon path-length probability density function (PPDF)-based method was adapted to correct for atmospheric scattering effects in CH(4) retrievals. This method was shown to provide similar retrieval accuracy as compared to a carbon dioxide (CO(2))-proxy-based correction approach. It infers some advantages of PPDF-based method for methane retrievals under high variability of CO(2) abundance.

  1. Introducing a new method for evaluation of the interaction between an antigen and an antibody: single frequency impedance analysis for biosensing systems.

    PubMed

    Özcan, Burcu; Demirbakan, Burçak; Yeşiller, Gülden; Sezgintürk, Mustafa Kemal

    2014-07-01

    This paper illustrates the application of an antibody, anti-parathyroid hormone (anti-PTH), as a bioreceptor in a biosensor system for the first time, and demonstrates how this biosensor can be used in parathyroid hormone (PTH) determination. The interaction between the biosensor and parathyroid hormone was firstly investigated by a novel electrochemical method, single frequency impedance analysis. The biosensor was based on the gold electrode modified by cysteine self-assembled monolayers. Anti-PTH was covalently immobilized onto cysteine layer by using an EDC/NHS couple. The immobilization of anti-PTH was monitored by cyclic voltammetry and electrochemical impedance spectroscopy techniques. The performance of the biosensor was evaluated in terms of linearity, sensitivity, repeatability and reproducibility, after a few important optimization studies were carried out. In particular, parathyroid hormone was detected within a linear range of 10-60 fg/mL. Kramers-Kronig transform was also performed on the impedance data. The specificity of the biosensor was also evaluated. The biosensor was validated by using a complementary reference technique. Lastly the developed biosensor was used to monitor PTH levels in artificial serum samples.

  2. Multimedia Information Retrieval Literature Review

    SciTech Connect

    Wong, Pak C.; Bohn, Shawn J.; Payne, Deborah A.

    2010-10-12

    This survey paper highlights some of the recent, influential work in multimedia information retrieval (MIR). MIR is a branch area of multimedia (MM). The young and fast-growing area has received strong industrial and academic support in the United States and around the world (see Section 7 for a list of major conferences and journals of the community). The term "information retrieval" may be misleading to those with different computer science or information technology backgrounds. As shown in our discussion later, it indeed includes topics from user interaction, data analytics, machine learning, feature extraction, information visualization, and more.

  3. Publication search and retrieval system

    USGS Publications Warehouse

    Winget, Elizabeth A.

    1981-01-01

    The publication search and retrieval system of the Branch of Atlantic-Gulf of Mexico Geology, U.S. Geological Survey, Woods Hole, Mass., is a procedure for listing and describing branch-sponsored publications. It is designed for maintenance and retrieval by those having limited knowledge of computer languages and programs. Because this branch currently utilizes the Hewlett-Packard HP-1000 computer with RTE-IVB operating system, database entry and maintenance is performed in accordance with the TE-IVB Terminal User’s Reference Manual (Hewlett-Packard Company, 1980) and within the constraints of GRASP (Bowen and Botbol, 1975) and WOLF (Evenden, 1978).

  4. Accelerated Adaptive MGS Phase Retrieval

    NASA Technical Reports Server (NTRS)

    Lam, Raymond K.; Ohara, Catherine M.; Green, Joseph J.; Bikkannavar, Siddarayappa A.; Basinger, Scott A.; Redding, David C.; Shi, Fang

    2011-01-01

    The Modified Gerchberg-Saxton (MGS) algorithm is an image-based wavefront-sensing method that can turn any science instrument focal plane into a wavefront sensor. MGS characterizes optical systems by estimating the wavefront errors in the exit pupil using only intensity images of a star or other point source of light. This innovative implementation of MGS significantly accelerates the MGS phase retrieval algorithm by using stream-processing hardware on conventional graphics cards. Stream processing is a relatively new, yet powerful, paradigm to allow parallel processing of certain applications that apply single instructions to multiple data (SIMD). These stream processors are designed specifically to support large-scale parallel computing on a single graphics chip. Computationally intensive algorithms, such as the Fast Fourier Transform (FFT), are particularly well suited for this computing environment. This high-speed version of MGS exploits commercially available hardware to accomplish the same objective in a fraction of the original time. The exploit involves performing matrix calculations in nVidia graphic cards. The graphical processor unit (GPU) is hardware that is specialized for computationally intensive, highly parallel computation. From the software perspective, a parallel programming model is used, called CUDA, to transparently scale multicore parallelism in hardware. This technology gives computationally intensive applications access to the processing power of the nVidia GPUs through a C/C++ programming interface. The AAMGS (Accelerated Adaptive MGS) software takes advantage of these advanced technologies, to accelerate the optical phase error characterization. With a single PC that contains four nVidia GTX-280 graphic cards, the new implementation can process four images simultaneously to produce a JWST (James Webb Space Telescope) wavefront measurement 60 times faster than the previous code.

  5. Comparisons of Upper Tropospheric Humidity Retrievals from TOVS and METEOSAT

    NASA Technical Reports Server (NTRS)

    Escoffier, C.; Bates, J.; Chedin, A.; Rossow, W. B.; Schmetz, J.

    1999-01-01

    Two different methods for retrieving Upper Tropospheric Humidities (UTH) from the TOVS (TIROS Operational Vertical Sounder) instruments aboard NOAA polar orbiting satellites are presented and compared. The first one, from the Environmental Technology Laboratory, computed by J. Bates and D. Jackson (hereafter BJ method), estimates UTH from a simplified radiative transfer analysis of the upper tropospheric infrared water vapor channel at wavelength measured by HIRS (6.3 micrometer). The second one results from a neural network analysis of the TOVS (HIRS and MSU) data developed at, the Laboratoire de Meteorologie Dynamique (hereafter the 3I (Improved Initialization Inversion) method). Although the two methods give very similar retrievals in temperate regions (30-60 N and S), an absolute bias up to 16% appears in the convective zone of the tropics. The two datasets have also been compared with UTH retrievals from infrared radiance measurements in the 6.3 micrometer channel from the geostationary satellite METEOSAT (hereafter MET method). The METEOSAT retrievals are systematically drier than the TOVS-based results by an absolute bias between 5 and 25%. Despite the biases, the spatial and temporal correlations are very good. The purpose of this study is to explain the deviations observed between the three datasets. The sensitivity of UTH to air temperature and humidity profiles is analysed as are the clouds effects. Overall, the comparison of the three retrievals gives an assessment of the current uncertainties in water vapor amounts in the upper troposphere as determined from NOAA and METEOSAT satellites.

  6. Multilayered Clouds Identification and Retrieval for CERES Using MODIS

    NASA Technical Reports Server (NTRS)

    Sun-Mack, Sunny; Minnis, Patrick; Chen, Yan; Yi, Yuhong; Huang, Jainping; Lin, Bin; Fan, Alice; Gibson, Sharon; Chang, Fu-Lung

    2006-01-01

    Traditionally, analyses of satellite data have been limited to interpreting the radiances in terms of single layer clouds. Generally, this results in significant errors in the retrieved properties for multilayered cloud systems. Two techniques for detecting overlapped clouds and retrieving the cloud properties using satellite data are explored to help address the need for better quantification of cloud vertical structure. The first technique was developed using multispectral imager data with secondary imager products (infrared brightness temperature differences, BTD). The other method uses microwave (MWR) data. The use of BTD, the 11-12 micrometer brightness temperature difference, in conjunction with tau, the retrieved visible optical depth, was suggested by Kawamoto et al. (2001) and used by Pavlonis et al. (2004) as a means to detect multilayered clouds. Combining visible (VIS; 0.65 micrometer) and infrared (IR) retrievals of cloud properties with microwave (MW) retrievals of cloud water temperature Tw and liquid water path LWP retrieved from satellite microwave imagers appears to be a fruitful approach for detecting and retrieving overlapped clouds (Lin et al., 1998, Ho et al., 2003, Huang et al., 2005). The BTD method is limited to optically thin cirrus over low clouds, while the MWR method is limited to ocean areas only. With the availability of VIS and IR data from the Moderate Resolution Imaging Spectroradiometer (MODIS) and MW data from the Advanced Microwave Scanning Radiometer EOS (AMSR-E), both on Aqua, it is now possible to examine both approaches simultaneously. This paper explores the use of the BTD method as applied to MODIS and AMSR-E data taken from the Aqua satellite over non-polar ocean surfaces.

  7. High dimensional model representation (HDMR) with clustering for image retrieval

    NASA Astrophysics Data System (ADS)

    Karcılı, Ayşegül; Tunga, Burcu

    2017-01-01

    Image retrieval continues to hold an important place in today's extremely fast growing technology. In this field, the accurate image retrieval with high speed is critical. In this study, to achieve this important issue we developed a novel method with the help of High Dimensional Model Representation (HDMR) philosophy. HDMR is a decomposition method used to solve different scientific problems. To test the performance of the new method we used Columbia Object Image Library (COIL100) and obtained the encouraging results. These results are given in the findings section.

  8. New Retrievable Coil Anchors: Preliminary In Vivo Experiences in Swine

    SciTech Connect

    Konya, A. Wright, K.C.

    2005-04-15

    Purpose. To design and test retrievable coil anchors to improve the safety and efficacy of coil embolization. Methods. Fifty-two 0.038-inch homemade retrievable stainless steel coils were equipped with one of four different pre-shaped nitinol anchors and tested in 38 pigs. All coils with the anchor were completely retrieved and redeployed 3-18 times (median 7 times) prior to release. Types 1 and 2 anchored coils were acutely deployed in the external iliac arteries (n = 10 each), and chronically tested (1 week) in the common carotid arteries (n = 6 each). Larger type 1 (n = 4), type 3 (n = 6), and type 4 (n = 4) anchored coils were acutely deployed in the abdominal aorta. The largest type 1 anchors (n = 6) were acutely tested in the inferior vena cava. Results. All anchored coils were successfully retrieved and repositioned several times. All but two coils formed a compact plug and there was no coil migration except with two mechanically defective type 3 anchors. Conclusion. The use of retrievable anchors allowed the coils to be retrieved and repositioned, prevented coil migration, and enabled compact coil configuration.

  9. Hanford contact-handled transuranic drum retrieval project planning document

    SciTech Connect

    DEMITER, J.A.

    1998-11-17

    The Hanford Site is one of several US Department of Energy (DOE) sites throughout the US that has generated and stored transuranic (TRU) wastes. The wastes were primarily placed in 55-gallon drums, stacked in trenches, and covered with soil. In 1970, the Nuclear Regulatory Commission ordered that TRU wastes be segregated from other radioactive wastes and placed in retrievable storage until such time that the waste could be sent to a geologic repository and permanently disposed. Retrievable storage also defined container storage life by specifying that a container must be retrievable as a contamination-free container for 20 years. Hanford stored approximately 37,400 TRU containers in 20-year retrievable storage from 1970 to 1988. The Hanford TRU wastes placed in 20-year retrievable storage are considered disposed under existing Resource Conservation and Recovery Act (RCRA) regulations since they were placed in storage prior to September 1988. The majority of containers were 55-gallon drums, but 20-year retrievable storage includes several TRU wastes covered with soil in different storage methods.

  10. Antigenic variation in Giardia lamblia.

    PubMed

    Prucca, Cesar G; Lujan, Hugo D

    2009-12-01

    Giardia lamblia undergoes antigenic variation, both in vitro and within the intestines of infected individuals. Variant-specific surface proteins (VSPs) cover the entire surface of the trophozoites and are the main antigens recognized by the host. Only 1 of about 200 VSP genes encoded by the Giardia genome is expressed on the surface of individual Giardia cells at any time; however, VSP antigen switching occurs spontaneously. In the recent year, significant advances in the knowledge of the antigen switching process have been achieved, which strongly suggests that antigenic variation in Giardia is regulated at the post-transcriptional level by a mechanism similar to RNA interference (RNAi). Several enzymes of the RNAi pathway are directly involved in VSP mRNA silencing and/or translational repression. Although several questions remain regarding how individual VSP antigens are selected for expression on the parasite surface, it is clear that an epigenetic mechanism is involved. In this review, we summarize the characteristics of this fascinating mechanism, analyse conflicting information regarding the structure of VSPs as it relates to the host's immune response, and highlight the major issues that need to be resolved to fully understand antigenic variation in this important pathogen.

  11. Serospecific antigens of Legionella pneumophila.

    PubMed Central

    Otten, S; Iyer, S; Johnson, W; Montgomery, R

    1986-01-01

    Serospecific antigens isolated by EDTA extraction from four serogroups of Legionella pneumophila were analyzed for their chemical composition, molecular heterogeneity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunological properties. The antigens were shown to be lipopolysaccharides and to differ from the lipopolysaccharides of other gram-negative bacteria. The serospecific antigens contained rhamnose, mannose, glucosamine, and two unidentified sugars together with 2-keto-3-deoxyoctonate, phosphate, and fatty acids. The fatty acid composition was predominantly branched-chain acids with smaller amounts of 3-hydroxymyristic acid. The antigens contain periodate-sensitive groups; mannosyl residues were completely cleaved by periodate oxidation. Hydrolysis of the total lipopolysaccharide by acetic acid resulted in the separation of a lipid A-like material that cross-reacted with the antiserum to lipid A from Salmonella minnesota but did not comigrate with it on sodium dodecyl sulfate gels. None of the four antigens contained heptose. All of the antigen preparations showed endotoxicity when tested by the Limulus amebocyte lysate assay. The results of this study indicate that the serogroup-specific antigens of L. pneumophila are lipopolysaccharides containing an unusual lipid A and core structure and different from those of other gram-negative bacteria. Images PMID:3017918

  12. Information Retrieval for Ecological Syntheses

    ERIC Educational Resources Information Center

    Bayliss, Helen R.; Beyer, Fiona R.

    2015-01-01

    Research syntheses are increasingly being conducted within the fields of ecology and environmental management. Information retrieval is crucial in any synthesis in identifying data for inclusion whilst potentially reducing biases in the dataset gathered, yet the nature of ecological information provides several challenges when compared with…

  13. Information Retrieval in Virtual Universities

    ERIC Educational Resources Information Center

    Puustjärvi, Juha; Pöyry, Päivi

    2006-01-01

    Information retrieval in the context of virtual universities deals with the representation, organization, and access to learning objects. The representation and organization of learning objects should provide the learner with an easy access to the learning objects. In this article, we give an overview of the ONES system, and analyze the relevance…

  14. Information Retrieval in the Classroom.

    ERIC Educational Resources Information Center

    Oley, Elizabeth

    1989-01-01

    Explores aspects of information retrieval skills such as end user training, indexing, controlled vocabulary systems, search protocol, boolean logic, problem analysis, and decision making. Suggests techniques for classroom instruction using simulations of online databases, CD-ROMs, and DIALOG's classroom instruction program. Describes several…

  15. INFORMATION RETRIEVAL EXPERIMENT. FINAL REPORT.

    ERIC Educational Resources Information Center

    SELYE, HANS

    THIS REPORT IS A BRIEF REVIEW OF RESULTS OF AN EXPERIMENT TO DETERMINE THE INFORMATION RETRIEVAL EFFICIENCY OF A MANUAL SPECIALIZED INFORMATION SYSTEM BASED ON 700,000 DOCUMENTS IN THE FIELDS OF ENDOCRINOLOGY, STRESS, MAST CELLS, AND ANAPHYLACTOID REACTIONS. THE SYSTEM RECEIVES 30,000 PUBLICATIONS ANNUALLY. DETAILED INFORMATION IS REPRESENTED BY…

  16. Hubble Space Telescope prescription retrieval.

    PubMed

    Redding, D; Dumont, P; Yu, J

    1993-04-01

    Prescription retrieval is a technique for directly estimating optical prescription parameters from images. We apply it to estimate the value of the Hubble Space Telescope primary mirror conic constant. Our results agree with other studies that examined primary-mirror test fixtures and results. In addition they show that small aberrations exist on the planetary-camera repeater optics.

  17. Photopolarimetric Retrievals of Snow Properties

    NASA Technical Reports Server (NTRS)

    Ottaviani, M.; van Diedenhoven, B.; Cairns, B.

    2015-01-01

    Polarimetric observations of snow surfaces, obtained in the 410-2264 nm range with the Research Scanning Polarimeter onboard the NASA ER-2 high-altitude aircraft, are analyzed and presented. These novel measurements are of interest to the remote sensing community because the overwhelming brightness of snow plagues aerosol and cloud retrievals based on airborne and spaceborne total reflection measurements. The spectral signatures of the polarized reflectance of snow are therefore worthwhile investigating in order to provide guidance for the adaptation of algorithms currently employed for the retrieval of aerosol properties over soil and vegetated surfaces. At the same time, the increased information content of polarimetric measurements allows for a meaningful characterization of the snow medium. In our case, the grains are modeled as hexagonal prisms of variable aspect ratios and microscale roughness, yielding retrievals of the grains' scattering asymmetry parameter, shape and size. The results agree with our previous findings based on a more limited data set, with the majority of retrievals leading to moderately rough crystals of extreme aspect ratios, for each scene corresponding to a single value of the asymmetry parameter.

  18. An Interactive Document Retrieval System.

    ERIC Educational Resources Information Center

    Carroll, D.E.; And Others

    The document retrieval system at Coordinated Science Laboratory is implemented on a Control Data 1604 computer system. Recorded information from each source article includes 1) conventional bibliographic data such as authorship, author affiliation, title, year of publication, etc., 2) detailed bibliographic data from each of the cited references,…

  19. Semantic Tools in Information Retrieval.

    ERIC Educational Resources Information Center

    Rubinoff, Morris; Stone, Don C.

    This report discusses the problem of the meansings of words used in information retrieval systems, and shows how semantic tools can aid in the communication which takes place between indexers and searchers via index terms. After treating the differing use of semantic tools in different types of systems, two tools (classification tables and…

  20. Cross-Language Information Retrieval.

    ERIC Educational Resources Information Center

    Oard, Douglas W.; Diekema, Anne R.

    1998-01-01

    Reviews research and practice in cross-language information retrieval (CLIR) that seeks to support the process of finding documents written in one natural language with automated systems that can accept queries expressed in other languages. Addresses user needs, document preprocessing, query formulation, matching strategies, sources of translation…