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Sample records for applying flagged sysmex

  1. Comparison of abnormal cell flagging of the hematology analyzers Sysmex XN and Sysmex XE-5000 in oncohematologic patients.

    PubMed

    Furundarena, J R; Sainz, M; Uranga, A; Cuevas, L; Lopez, I; Zubicaray, J; Bizjak, A; Robado, N; Araiz, M

    2017-02-01

    Hematology analyzers should optimize flagging while minimizing false-negative results and unnecessary microscopic reviews. We compared flagging performance of Sysmex XE-5000 and XN analyzers in oncohematologic patients. Differential counts were performed by Cellavision digital system (100 cells) and a hematologist (another 100 cells). First, we included 292 samples (86 with blasts): 28 acute lymphoblastic leukemia, 88 acute myeloid leukemia, 91 myelodysplastic syndromes, 45 chronic myeloproliferative neoplasms, and 40 chronic myelomonocytic leukemia. Sensitivity, specificity and efficiency to detect blasts were 59.3%, 88.3%, and 79.8% for XE-5000 analyzer and 70.9%, 91.3%, and 85.2% for the XN analyzer. Then, we included 111 lymphoid malignancies. In 55 CLL XE-5000 flagged for Abn Lympho/L_Blasts?, XN flagged for Abn Lympho?. In one-third of 19 samples with splenic marginal lymphoma, none of the analyzers flagged. In 5 Sézary syndrome cases, XE-5000 triggered the Abn Lympho/L_Blasts? flag while the flagging in XN was less consistent: Abn Lympho? Blasts? and Atypical Lympho?. In 5 hairy cell leukemias, both analyzers only flagged one sample. In 13 myelomas, XE-5000 generated Atypical Lympho? flag; XN triggered more variable flags. In other lymphoid malignancies, flags were variable. XN analyzer generates less samples with false basophilia. XN analyzer has improved blast detection in oncohematologic patients. Operators cannot rely on the blast flag alone but have to consider other flags and hemogram data. In lymphoproliferative disorders, XN analyzer yields less samples with pseudobasophilia. Both analyzers must improve flagging for hairy cell leukemia. © 2016 John Wiley & Sons Ltd.

  2. The usefulness of blast flags on the Sysmex XE-5000 is questionable.

    PubMed

    Eilertsen, Heidi; Vøllestad, Nina K; Hagve, Tor-Arne

    2013-05-01

    Hematology analyzers generate suspect flags that involve microscopic reviews to confirm the presence of pathologic cells. This study investigated the reliability of the blast flag in a side-by-side evaluation of 3 Sysmex XE-5000 instruments (Sysmex, Kobe, Japan). The repeatability of the Q values reported by each instrument for 10 replicates of the same blood samples was low (intraclass correlation coefficient [ICC] values, 0.62-0.74). The reproducibility of the Q values obtained by analyzing 408 samples on all 3 instruments was reasonable (ICC value, 0.85). In addition, a systematic difference was observed among the instruments in the level of reported Q values. With cutoff commonly being 100, the observed reproducibility of the blast flagging among the instruments was evaluated as poor (κ = 0.73). Based on the observed low performances, we question the usefulness of the Q value as a predictor of blasts and whether a blast flag reported by the XE-5000 is sufficient as a criterion for performing a microscopic review.

  3. Two cases of false platelet clumps flagged by the automated hematology analyzer Sysmex XE-2100.

    PubMed

    Dai, Qingkai; Zhang, Ge; Lai, Chunqi; Du, Zeli; Chen, Lan; Chen, Qi; Peng, Leiwen; Wang, Yuefang; Yang, Hui; Ye, Lei; Yang, Xianjun; Jiang, Yongmei

    2014-02-15

    Automated hematology analyzer is widely used by clinical laboratories to examine blood cell counts and differential leukocyte counts. However, a microscopic examination of a well-prepared blood film is still necessary and useful in most cases. Two patients with severe disease were required complete blood counts (CBC) after admission at West China Second University Hospital in Southwest China. A false "platelet clumps?" flag was generated by the automated hematology analyzer Sysmex XE-2100. However, when the well-prepared blood film was reviewed, there was echinocytosis rather than clumps of platelets. In addition, both patients had severe metabolic acidosis with arterial blood pH <7.00 and HCO3- <4 mmol/l. On day 2 of admission, the false platelet clumps flags were not presented in both cases. Our further results suggested that the false platelet clumps had been flagged probably due to the influence of the extremely low blood pH. These two cases emphasized that a microscopic examination of a blood film was central to the morphology of blood cells. When the result of microscopic examination was abnormal, it was suggested that the physician should consider further testing to find underlying condition. Copyright © 2013 Elsevier B.V. All rights reserved.

  4. Do the flags related to immature granulocytes reported by the Sysmex XE-5000 warrant a microscopic slide review?

    PubMed

    Eilertsen, Heidi; Hagve, Tor-Arne

    2014-10-01

    The Sysmex XE-5000 instruments (Sysmex, Kobe, Japan) count immature granulocytes (IGs) and use the "Imm Gran?" flag to signal unreliable results. This study investigated the usefulness of the "Imm Gran?" flag and the analytical and diagnostic performance of the IG measurements in a side-by-side evaluation. In total, 408 samples were analyzed on three XE-5000 instruments. The IG count and the "Imm Gran?" flag reports from all three instruments were used for reproducibility studies. The diagnostic performance of the automated IGs and the "Imm Gran?" flag were studied by comparing the XE-5000 results with the results of the manual differential. The reproducibility of the "Imm Gran?" flagging between instruments was poor (қ, 0.75-0.80). The most significant contributor to the report of the "Imm Gran?" flag was bands, and the flag played a minor role in detecting blasts. The interinstrument reproducibility of the IG counts was high (intraclass correlation, 0.99). The IG count reported by XE-5000s was higher than the manual IG count (36%-55%), and the difference and the variability tended to increase with increasing levels of IGs. The "Imm Gran?" flag has a poor analytical quality and gives no substantial information on the presence of blasts in the sample. We therefore suggest reporting the automated IG count without initial microscopic slide review. Copyright© by the American Society for Clinical Pathology.

  5. Evaluation of the Flagging Performance of the Hematology Analyzer Sysmex XN Series on the Basis of "Q Values".

    PubMed

    Kweon, Oh Joo; Lee, Mi-Kyung; Kim, Hye Ryoun

    2017-08-31

    - In the XN series of hematology analyzers (Sysmex, Kobe, Japan), the probability of the presence of abnormal cells is indicated by flags based on Q values. - To evaluate the Q values performance of the Sysmex XN-20 modular analyzer. - The interinstrumental concordance, intrainstrumental precision, and diagnostic accuracy of Q values, with tested flags of "blasts/abnormal lymphocytes," "atypical lymphocytes," and "blasts," were investigated. - Absolute concordance rates in flagging between 2 analyzers ranged from 69.8% to 80.8%, and κ values ranged from 0.43 to 0.61. In samples with absolute related cell counts lower than 100/μL, the values ranged from 0.31 to 0.52. For intrainstrumental precision, standard deviations ranged from 4.8 to 23.9 for the blasts/abnormal lymphocytes, from 18.7 to 59.1 for the blasts, and from 11.0 to 23.0 for the atypical lymphocytes. Using a default Q value cutoff, diagnostic accuracy values based on the area under the curve, sensitivity, and specificity were, respectively, 0.910, 90.9%, and 72.2% for blasts/abnormal lymphocytes; 0.927, 84.9%, and 89.8% for blasts; and 0.865, 74.4%, and 84.9% for atypical lymphocytes. The diagnostic accuracy of Q values was much lower in samples with absolute related cell counts lower than 100/μL than in those 100/μL or higher. - Q values of the Sysmex XN-20 analyzer were found to be imprecise and irreproducible, especially with samples containing a small number of pathologic cells. Adjustments in the Q value threshold may help in the detection of these cells.

  6. Automated five-part white blood cell differential counts. Efficiency of software-generated white blood cell suspect flags of the hematology analyzers Sysmex SE-9000, Sysmex NE-8000, and Coulter STKS.

    PubMed

    Thalhammer-Scherrer, R; Knöbl, P; Korninger, L; Schwarzinger, I

    1997-06-01

    The present study evaluates the efficiency of software-generated white blood cell (WBC) "suspect flags" of the hematology analyzers Sysmex SE-9000, Sysmex NE-8000, and Coulter STKS. Automated WBC differential counts were considered positive if they contained any suspect WBC flag indicating the presence of blasts, myeloid precursor cells, or abnormal lymphocytes. Reference differential counts were performed by microscopic examination of 400 WBCs per sample. After comparison to the reference method, automated differential counts were classified as true-positive, true-negative, false-positive, and false-negative. The flagging efficiency of analyzers was expressed as a percentage of subjects correctly classified. Four hundred sixty-seven blood samples were randomly chosen for comparison analysis from various inpatient and outpatient departments of the Vienna university hospital, Austria. The efficiency rates of flagging for the presence of > or = 1% abnormal WBCs were 78% (SE-9000), 77% (NE-8000), and 72% (Coulter STKS). The flagging efficiencies were best for samples with normal WBC counts. With regard to the specific suspect flags, the flagging of blast cells was most efficient on all analyzers. Our results demonstrate the comparable overall performance of three analyzers, SE-9000, NE-8000, and Coulter STKS. They further underscore the importance of critical interpretation of automated differential counts, because at a detection limit of > or = 1% abnormal WBCs > 20% of samples were not correctly flagged by either analyzer.

  7. Improved flagging rates on the Sysmex XE-5000 compared with the XE-2100 reduce the number of manual film reviews and increase laboratory productivity.

    PubMed

    Briggs, Carol J; Linssen, Joachim; Longair, Ian; Machin, Samuel J

    2011-08-01

    Hematology analyzers generate suspect flags in the presence of abnormal cells. False-positive rates for flags are high on all analyzers. Sysmex, Kobe, Japan, has developed new software for its XE-5000 with improved algorithms for flagging blast cells, abnormal lymphocytes or lymphoblasts, and atypical lymphocytes. This study evaluated the efficiency of these flags in 1,002 samples. The XE-5000 was compared with the XE-2100 (Sysmex) and microscopic examination of cell morphologic features. On the XE-2100, the blast flag demonstrated 90 false-positives, 13 true-positives, and 3 false-negatives. The values on the XE-5000 were 27 false-positives, 14 true-positives, and 2 false-negatives. The abnormal lymphocyte/lymphoblast flag was assessed with the atypical lymphocyte flag. The XE-2100 showed 114 false-positives, 23 true-positives, and 20 false-negatives, and on the XE-5000, there were 45 false-positives, 22 true-positives, and 21 false-negatives. This more specific flagging reduces the number of films that require manual review.

  8. [Laboratory evaluation of the Sysmex NE-7000 flagging system; a tool for the recognition of abnormal cells in white cell differentials].

    PubMed

    Kondo, S; Yamaguchi, N; Imanishi, T; Sakota, H; Jokei, Y; Mituda, A

    1991-06-01

    Sysmex NE-7000 flagging system was evaluated for the recognition of abnormal cells in white cell differentials. Six flags that could mark blasts, immature granulocytes, left shift, nucleated red blood cells, atypical lymphocytes, and platelet clumps were selected for evaluation. 182 morphologic abnormal samples were screened from the Kobe University Hospital patients, by means of the 600 eye counts differentials. Blasts flag missed 8 of 28 cases, and immature granulocytes flag missed 25 of 56 cases. These missed cases contained either relatively few abnormal cells; less than 3% immature granulocytes, or absolutely few cells; less than 201 per micro liter blasts. Platelet clumps flag missed only 2 of 23 cases. But nucleated red blood cells and atypical lymphocytes flags missed all of 7 and 4 cases respectively. Left shift flag missed 36 of 64 cases, but it could help marking immature granulocytes or blasts. To use the NE-7000 as a screening tool, we compared the NE's all 35 flags versus abnormal cells or status based on the 100 eye counts. 1,153 cases were analyzed. Full agreement; 1047 (90.8%), partial agreement; 61 (5.2%), distributional false normal; 7 (1.6%), and morphological false normal; 4 (4.6%), were obtained. The more flags were used, the less abnormal cases were missed. But it accompanied the increase of the flag positive cases that must be confirmed by eye.

  9. The use of CellaVision™ DM96 in the verification of the presence of blasts in samples flagged by the Sysmex XE-5000.

    PubMed

    Eilertsen, H; Henriksson, C E; Hagve, T-A

    2017-08-01

    The CellaVision™DM96 is a digital pattern recognition system that classifies white blood cells. The aim of this study was to evaluate whether the CellaVision preclassification feature, without a subsequent re-classification, was a sufficient approach to follow up flags reported by Sysmex XE-5000. Pairs of blood smears from 400 samples reported with suspect flags were examined using conventional microscopy and the CellaVision features. The effect of pre- vs. re-classification, and intersmear and between-technologist variation, on blast counts was assessed using generalized linear mixed models (GLMM). The GLMM analysis showed a significant difference between the blast counts of preclassification vs. re-classification (P = 0.009). The analysis showed no significant difference between duplicate smears (P = 0.621) or between technologists (P = 0.542). Preclassification showed blasts in 105 samples (26%), where the re-classification feature did not detect any blasts. Not a single sample that was re-classified as positive for blasts was preclassified as negative. Compared to manual microscopy, the sensitivity and specificity of the preclassification feature were 0.83 and 0.66, respectively. The preclassification feature alone is sufficient to verify the absence of blasts in flagged samples. When the preclassification feature detects blasts, the finding has to be verified or reclassified by an experienced technologist. However, the use of CellaVision™DM96 in the follow-up of blast reports has to be questioned due to the finding of false-negative samples in the preclassification feature, but also after re-classification, compared to manual slide review. © 2017 John Wiley & Sons Ltd.

  10. Synchronized switch harvesting applied to piezoelectric flags

    NASA Astrophysics Data System (ADS)

    Piñeirua, Miguel; Michelin, Sébastien; Vasic, Dejan; Doaré, Olivier

    2016-08-01

    In this article the energy transfer between a flow and a fluttering piezoelectric plate is investigated. In particular, the benefits of the use of a synchronized switch harvesting on inductor (SSHI) circuit are studied. Both wind tunnel experiments and numerical simulations are conducted in order to analyze the influence of the switching process on the dynamics and the efficiency of the system. Numerical simulations consist of a weakly nonlinear model of a plate in axial flow equipped with a single pair of piezoelectric patches, discretized using a Galerkin method where basis functions are the modes of the plate in vacuum. The discretized model is then integrated in time. The results presented in this paper show that a significant improvement of the harvested energy can be obtained using SSHI circuits compared to basic resistive circuits. It is also shown that for strongly coupled systems, the switching process inherent to he SSHI circuit has a significant impact on the dynamics of the flag, which tends to decrease the relative efficiency gain.

  11. Evaluation and optimization of the extended information process unit (E-IPU) validation module integrating the sysmex flag systems and the recommendations of the French-speaking cellular hematology group (GFHC).

    PubMed

    Cornet, Edouard; Mullier, François; Despas, Noemie; Jacqmin, Hugues; Geara, Carole; Boubaya, Marouane; Chatelain, Bernard; Troussard, Xavier

    2016-10-01

    The French-Speaking Cellular Haematology Group (GFHC) recently published criteria for microscopic analysis of a blood smears when a hemogram is requested. In order to evaluate and improve these recommendations using an XN (Sysmex) analyzer, we assessed 31,836 samples categorized into two sub-groups of patients either receiving or not receiving care in the clinical hematology/oncology departments of two university hospitals. By combining the manufacturer's recommendations and the GFHC recommendations, 21.3% of samples had a positive review flag in phase 1 of our study (17,991 samples). In phase 2 (13,845 samples), increasing the immature granulocytes (IG) percentage from 5-10% as a review trigger threshold, and ignoring slides with isolated flags 'PLT HIGH' (thrombocytosis) or 'MCV LOW' (microcytosis) or 'Blast/Abn Lymph and Atypical Lymph' (blast cells/abnormal lymphocytes and atypical lymphocytes) (in the absence of abnormal cells on a previous blood smear within 72 h), enabled us to significantly reduce the number of slides reviewed from 21.3-15.0% (p < 0.0001), without loss of clinical value. This decrease occurred in both sub-groups (hematology 48.7-38.0%, non-hematology 18.3-11.7%, p < 0.0001). In conclusion, the application of the GFHC criteria adapted to XN analyzers has enabled us to optimize the hematology laboratory processes, and thus reduce the production costs and the turnaround time of hemogram results.

  12. COOPER, HERON, AND HEWARD'S APPLIED BEHAVIOR ANALYSIS (2ND ED.): CHECKERED FLAG FOR STUDENTS AND PROFESSORS, YELLOW FLAG FOR THE FIELD

    PubMed Central

    Friman, Patrick C

    2010-01-01

    At last, the field of applied behavior analysis has a beautifully crafted, true textbook that can proudly stand cover to cover and spine to spine beside any of the expensive, imposing, and ornately designed textbooks used by college instructors who teach courses in conventional areas of education or psychology. In this review, I fully laud this development, credit Cooper, Heron, and Heward for making it happen, argue that it signifies a checkered flag for students and professors, and recommend the book for classes in applied behavior analysis everywhere. Subsequently, I review its chapters, each of which could easily stand alone as publications in their own right. Finally, I supply a cautionary note, a yellow flag to accompany the well-earned checkered flag, by pointing out that, as is true with all general textbooks on applied behavior analysis, a major portion of the references involves research on persons who occupy only a tail of the normal distribution. To attain the mainstream role Skinner envisioned and most (if not all) behavior analysts desire, the field will have to increase its focus on persons who reside under the dome of that distribution.

  13. Performance evaluation of the Sysmex XS-1000i automated haematology analyser.

    PubMed

    Ghys, T; Malfait, R; VAN den Bossche, J

    2009-10-01

    The Sysmex XS-1000i is a compact new, fully automated haematology analyser, designed to generate complete blood counts with five-part leucocyte differential. In our study, a Sysmex XS-1000i instrument was evaluated according to Clinical Laboratory Standards Institute (CLSI) and International Council for Standardization in Haematology (ICSH) guidelines. Precision, carry-over and linearity were determined. Using a total of 700 patient samples, results from the Sysmex XS-1000i were compared with those from a Sysmex XE-2100, an Abbott Cell Dyn 4000 and the manual reference leucocyte differential. Using quality control material, total and within-run imprecision was less than 3% except for platelets. The system demonstrated good linearity over the entire reporting range and no carry-over (<0.5%). The Sysmex XS-1000i showed good correlation with XE-2100, CD-4000 and the manual reference leucocyte differential. Overall flagging sensitivity and specificity were 91% and 48%, respectively. In conclusion, the Sysmex XS-1000i demonstrated good analytical performance, is able to generate a complete blood count with five-part differential on low blood volumes and has considerable back-up capacity.

  14. A novel immersed boundary method applied to the inverted flag problem

    NASA Astrophysics Data System (ADS)

    Goza, Andres; Colonius, Timothy

    2015-11-01

    This work uses a 2-D immersed boundary method to study the inverted flag problem, in which a deformable flag is pinned at the trailing edge with its leading edge free to flap. Compared with the canonical flag problem, the inverted configuration more readily undergoes large flapping behavior for a wide range of mass ratios, making it promising in the field of flow-energy harvesting. A previous study identified several flapping modes as a function of dimensionless mass and stiffness ratios (D. Kim et al., J. Fluid Mech., 2013). The present work investigates the role of vortex formation and wake-flag interaction on the different flapping regimes. Simulations are performed using a 2-D immersed boundary method that accurately computes surface stresses imposed on the body by the fluid boundary conditions. Unlike many immersed boundary methods that reconstruct surface stresses from the velocity field, the current method only uses information from the immersed surface, leading to a smaller algebraic system for the fluid-solid coupling. The large flapping behavior of the inverted flag problem highlights the method's ability to simulate flows around bodies undergoing large deformations. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE?1144469.

  15. The value of the white precursor cell channel (WPC) on the Sysmex XN-1000 analyser in a specialist paediatric hospital.

    PubMed

    Jones, Amanda S; Tailor, Hitesh; Liesner, Ri; Machin, Samuel J; Briggs, Carol J

    2015-02-01

    Historically, haematology analyser flags for abnormal white blood cells (WBCs) show good sensitivity but lower specificity, causing unnecessary blood film reviews. While the WBC differential channel on Sysmex XE and XN instruments reports a combined flag for blasts/abnormal lymphocytes, the new white precursor cell channel (WPC) on the XN series has been introduced to separate this into a specific flag for either cell type or, if no abnormality, remove the flag entirely. To compare the efficiency of abnormal WBC flags from the XN WPC to our existing analyser and determine whether WPC can reduce false positive flags and blood films required. Abnormal WBC flags from the Sysmex XE-5000 and XN-1000 were compared to manual differential and blood film morphology on 300 K2EDTA samples from infants and children. The XN WPC flag for blasts was more sensitive and specific than flags indicating blasts on the XE-5000, with a reduction in false positives from 64% (XE) to 36% (XN). Overall efficiency of the WPC flag for abnormal lymphocytes was 94% vs 79% on the XE. WPC reduced false positive flags for blasts and abnormal lymphocytes on neonatal samples by 50%. Automatic reflex analysis by WPC correctly removed a false positive flag from the white cell differential channel on 46% of samples. Total abnormal WBC flags from XN WPC were less (73) than the XE-5000 (92). XN WPC demonstrated superior efficiency of abnormal WBC flags on paediatric samples, compared to the XE-5000, with greater sensitivity and specificity of flagging, reducing blood films for review. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  16. Performance evaluation of the white cell precursor channel on the Sysmex XN hematology analyzer in HIV specimens.

    PubMed

    Schapkaitz, Elise; Khoza, Phoyisile

    2017-09-26

    Automated hematology analyzers generate white blood cell (WBC) suspect flags to indicate smears requiring manual review. In a human immunodeficiency virus (HIV) patient population with a high frequency of abnormal specimens, the usefulness of these flags depends on their sensitivity and specificity. The white cell precursor (WPC) channel on the Sysmex XN-9000 analyzer (Sysmex Corporation, Kobe, Japan) has recently been introduced in order to distinguish immature cells such as blasts and abnormal lymphocytes. The efficiency of the flags generated by WPC reflex testing was compared to the white cell differential (WDF) on the Sysmex XN-9000 in 400 routine HIV samples. A flag was generated by the WDF channel in 346 (86.50%) samples. Addition of the WPC to the WDF resulted in a 20.99% reduction in the smear review rate. WPC analysis correctly identified the initial WDF flag in 59 (60.82%) samples with 'blasts?' or 'abnormal lympho?' flags. WPC showed an excellent sensitivity of 100%. However, the specificity remained poor. On investigation, samples with chronic infection/inflammation (35.00%) and lymphopenia (63.25%) were associated with a high false positive rate of 76.32% and 55.56%, respectively. WPC analysis removed 45 (51.72%) coexisting 'atypical lympho?' false positive WDF flags. The 'atypical lympho?' flag showed an increased sensitivity and specificity of 88.46% and 74.67%, respectively. White cell precursor reflex testing showed improved flagging efficiency for the 'atypical lympho?' flag, which was a good indicator of reactive lymphocytes during chronic infection/inflammation. Nevertheless, the false positive rate for 'abnormal lympho/blasts?' was high in the presence of lymphopenia and infection. © 2017 Wiley Periodicals, Inc.

  17. Performance evaluation of the Sysmex haematology XN modular system.

    PubMed

    Briggs, Carol; Longair, Ian; Kumar, Punamar; Singh, Deepak; Machin, Samuel J

    2012-11-01

    The Sysmex XN haematology instrument performs automatic reflex testing, depending on sample results. A nucleated red blood cell (NRBC) count is provided on all samples. The instrument has a smaller footprint (34%) than previous Sysmex XE analysers. An evaluation comparing all results to the Sysmex XE-2100 and manual microscopic differential and morphology (n=390) was performed followed by a workflow study of 1000 samples to compare speed of operation and number of blood films reviews required from both systems. The new features on the instrument are: (1) white cell and NRBC channel, all samples include the NRBC count; (2) white cell precursor channel: false positive flags for blasts, abnormal lymphocytes and atypical lymphocytes are reduced significantly without a statistical increase of false negatives; (3) low white cell count mode: suggested setting of <0.5×10(9)/l. An extended count is more precise and provides an accurate differential. Fluorescent platelet count is performed in a dedicated channel. If the red cell or platelet size histograms are abnormal or if the platelet count is low, then a fluorescent platelet count is automatically performed. Good correlation with the XE-2100 and manual differential was found and the improved results compared to the reference flow cytometric analysis for platelet counts, especially below 30×10(9)/l (XE-2100, R(2)=0.500; XN, R(2)=0.875). The XN showed reduced sample turnaround time of 10% and reduced number of blood films for examination, 49% less than the XE-2100 without loss of sensitivity with more precise and accurate results on low cell counts.

  18. Analytic errors in Sysmex-generated hematology results in blood from a dog with chronic lymphocytic leukemia.

    PubMed

    Novacco, Marilisa; Martini, Valeria; Grande, Carmen; Comazzi, Stefano

    2015-09-01

    A blood sample from a 14-year-old dog was submitted to the veterinary diagnostic laboratory of the University of Milan for marked leukocytosis with atypical cells. A diagnosis of chronic T-cell lymphocytic leukemia (CLL) was made based on blood smear evaluation and flow cytometric phenotyping. A CBC by Sysmex XT-2000iV revealed a moderate normocytic normochromic anemia. Red blood cells counted by optic flow cytometry (RBC-O) resulted in a higher value than using electrical impedance (RBC-I). The relative reticulocyte count based on RNA content and size was 35.3%, while the manual reticulocyte count was < 1%. The WBC count of 1,562,680 cells/μL was accompanied by a flag. Manual counts for RBC and WBC using the Bürker chamber confirmed the Sysmex impedance results. Finally the manual PCV was lower than HCT by Sysmex. While Sysmex XT can differentiate between RBC and WBC by impedance, even in the face of extreme lymphocytosis due to CLL, RBC-O can be affected by bias, resulting in falsely increased RBC and reticulocyte numbers. Overestimation of RBC-O may be due to incorrect Sysmex classification of leukemic cells or their fragments as reticulocytes. This phenomenon is known as pseudoreticulocytosis and can lead to misinterpretation of regenerative anemia. On the other side PCV can be affected by bias in CLL due to the trapping of RBC in the buffy coat, resulting in a pink hue in the separation area. As HGB concentration is not affected by flow cytometric or other cell-related artifacts it may represent the most reliable variable to assess the degree of anemia in cases of CLL.

  19. Performance evaluation of the Sysmex(®) XP-300 in an oncology setting: evaluation and comparison of hematological parameters with the Sysmex(®) XN-3000.

    PubMed

    van Dievoet, M A; Louagie, H; Ghys, T

    2016-10-01

    The Sysmex XP-300(®) (XP-300) is a new, fully automated hematology analyzer, designed to generate complete blood counts (CBC) with 3-part differential. In our study, the XP-300 was evaluated as a point-of-care (POC) analyzer in an oncology setting. In which blood samples from patients with different pathologies and treatments, affecting hematopoiesis, were analyzed. Performance was evaluated according to the International Council for Standardization in Haematology (ICSH) guidelines and CLSI protocol H20-A2 . Beside precision, linearity and carry-over, a comparison study with the Sysmex(®) XN-3000 (XN-3000) and a manual reference leukocyte differential was performed. Flagging performance was also evaluated. XP-300 showed excellent precision and linearity results. For within- and between-run precision, the criteria, according to Ricos et al. , were met for all parameters tested, except for platelets in the low level. Less than or equal to 0.5% carry-over was seen for all parameters tested. Comparison studies showed an acceptable correlation with both XN-3000 and the manual reference leukocyte count. A suboptimal flagging performance was demonstrated. In the context of diagnosing cytopenia due to myelosuppressing agents or leukocytosis due to infection, the XP-300 showed good analytical performance. However, in the thrombocytopenic range, precision was suboptimal. In follow-up of hematological malignancies with the occurrence of abnormal cells, we advise verification with a more advanced analyzer or with microscopic review, although further studies with a higher prevalence of abnormal cells are needed. © 2016 John Wiley & Sons Ltd.

  20. A method for optimizing and validating institution-specific flagging criteria for automated cell counters.

    PubMed

    Sireci, Anthony; Schlaberg, Robert; Kratz, Alexander

    2010-10-01

    Automated cell counters use alerts (flags) to indicate which differential white blood cell counts can be released directly from the instrument and which samples require labor-intensive slide reviews. The thresholds at which many of these flags are triggered can be adjusted by individual laboratories. Many users, however, use factory-default settings or adjust the thresholds through a process of trial and error. To develop a systematic method, combining statistical analysis and clinical judgment, to optimize the flagging thresholds on automated cell counters. Data from 502 samples flagged by Sysmex XE-2100/5000 (Sysmex, Kobe, Japan) instruments, with at least 1 of 5 user-adjustable, white blood cell count flags, were used to change the flagging thresholds for maximal diagnostic effectiveness by optimizing the Youden index for each flag (the optimization set). The optimized thresholds were then validated with a second set of 378 samples (the validation set). Use of the new thresholds reduced the review rate caused by the 5 flags from 6.5% to 2.9% and improved the positive predictive value of the flagging system for any abnormality from 27% to 37%. This method can be used to optimize thresholds for flag alerts on automated cell counters of any type and to improve the overall positive predictive value of the flagging system at the expense of a reduction in the negative predictive value. A reduced manual review rate helps to focus resources on differential white blood cell counts that are of clinical significance and may improve turnaround time.

  1. Erroneously elevated immature reticulocyte counts in leukemic patients determined using a Sysmex XE-2100 hematology analyzer.

    PubMed

    Huh, Jungwon; Moon, Heewon; Chung, Whasoon

    2007-10-01

    The immature reticulocyte fraction (IRF) in peripheral blood, as determined by automated reticulocyte analysis, is calculated using the sum of medium and highly fluorescent reticulocyte numbers and provides information about erythropoietic activity in bone marrow. The purpose of this study was to investigate erroneously elevated IRF in leukemic patients, as determined using a Sysmex XE-2100 hematology analyzer (Sysmex, Kobe, Japan). Normal reticulocyte scattergram patterns show regions corresponding to reticulocytes located between matured RBCs and an upper particle (UPP) region, which show a continuum of non-separated fraction. The UPP represents erythroblasts and some immature reticulocytes. As a control group, peripheral blood was taken from patients with benign hematologic diseases, and their reticulocyte scattergrams all showed a normal pattern; UPP values were all less than 100. However, the reticulocyte scattergrams of 5 of 11 leukemia patients showed abnormal patterns and displayed a gap between RBC and reticulocyte regions. Three patients showed a flag with a message such as "RET Abn Scattergram". IRF results were elevated in these five patients, and their UPP values were above 100. For the remaining six leukemia patients with a normal reticulocyte scattergram pattern, immature reticulocytes were not markedly increased, and UPP values were less than 100. The findings of the present study demonstrate that IRF results may be erroneously elevated in leukemia patients and indicate that hematologists should examine reticulocyte scattergrams and UPP values carefully.

  2. Expedition 8 Flag Raising

    NASA Image and Video Library

    2003-10-14

    The flags of the United States, Russia, Spain and Kazakhstan fly outside the Cosmonaut Hotel, Tuesday, Oct. 14, 2003, in Baikonur, Kazakhstan following a traditional flag-raising ceremony. Photo Credit (NASA/Bill Ingalls)

  3. Automated Approaches to RFI Flagging

    NASA Astrophysics Data System (ADS)

    Garimella, Karthik; Momjian, Emmanuel

    2017-01-01

    It is known that Radio Frequency Interference (RFI) is a major issue in centimeter wavelength radio astronomy. Radio astronomy software packages include tools to excise RFI; both manual and automated utilizing the visibilities (the uv data). Here we present results on an automated RFI flagging approach that utilizes a uv-grid, which is the intermediate product when converting uv data points to an image. It is a well known fact that any signal that appears widespread in a given domain (e.g., image domain) is compact in the Fourier domain (uv-grid domain), i.e., RFI sources that appear as large scale structures (e.g., stripes) in images can be located and flagged using the uv-grid data set. We developed several automated uv-grid based flagging algorithms to detect and excise RFI. These algorithms will be discussed, and results of applying them to measurement sets will be presented.

  4. Local verification between the hematological analyzers Sysmex XN-series and XE-5000.

    PubMed

    Pérez, I; Redín, M E; Vives, A; Garrido, A; Urrechaga, E; Lacasta, M

    2016-06-01

    The aim of this study was to perform a verification of the hematology analyzer Sysmex XN-2000 by comparing with the previous XE-5000. This study assessed the precision and carryover on the XN-2000 and the systematic error between the both counters according to desirable biological variability criterion and a flag comparison study. Within-run precision and between-batch precision were measured according to the ICSH guidelines. A comparative study was performed analyzing two hundred and six samples of peripheral blood from patients. The statistical study was conducted using the Passing-Bablok and Bland-Altman analyses. The leucocyte flag comparison was made by measuring the efficiency rate. Between-batch precision was lower than that recommended by the biological variability criterion and manufacturer specifications. The comparison gave nonagreement results for neutrophil and basophil counts according to the criterion of biological variability. Erythroblasts and immature granulocytes showed nonagreement, but there is no available biological variation database for these parameters to compare with. Nevertheless, excellent absolute agreement was found for red blood cell parameters, and for platelet, lymphocyte, monocyte, and eosinophil counts. The global results obtained for the precision, comparability, and efficiency provide a satisfactory integration of the XN-2000 in the core laboratory routine and accomplish an optimal reliability. © 2016 John Wiley & Sons Ltd.

  5. An evaluation of the Sysmex NE-8000 hematology analyzer.

    PubMed

    Hallawell, R; O'Malley, C; Hussein, S; Dauer, R J; Tanti, M; Wootton, A M; McGrath, K M

    1991-11-01

    The Sysmex NE-8000 is a new, fully automated hematology analyzer capable of providing a five-part white blood cell differential count and identifying abnormal specimens. This instrument was evaluated on 5,000 consecutive blood specimens and compared to the Coulter S Plus-IV analyzer and manual differential cell counts to determine the efficacy of its five-cell differential and screening capabilities. There was a high correlation between the commercial counters for the standard parameters, white blood cell count, red blood cell count, hemoglobin level, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, and platelet count (r greater than 0.95), except for the mean corpuscular hemoglobin concentration (r = 0.51), for which the NE-8000 was considered the more accurate measurement. Precision and linearity studies were excellent. The white blood cell count, red blood cell count, hemoglobin level, and platelet count were reproducible on specimens stored at 4 degrees C or room temperature for 72 hours and the differential counts were reproducible for 12 hours. The correlations between automated and manual counts for neutrophils, eosinophils, basophils, and lymphocytes were excellent: r = 0.912, 0.945, 0.332, and 0.964, respectively. The monocyte correlation improved with software modification from 0.306 to 0.801. The NE-8000 gave accurate and reproducible differential counts for neutrophils, lymphocytes, and monocytes on specimens with white blood cell counts as low as 0.6 x 10(9)/1. The ability of the instrument to 'flag' abnormal specimens was excellent. The false-positive rate on normal samples was 1.8%, and the false-negative rate on known abnormal samples was 0.3%, due only to nonrecognition of a mild left shift. The identification of specific abnormalities was less precise. The NE-8000 is a powerful hematology analyzer that can perform a five-part white blood cell differential count accurately for a wide range of WBCs and reliably

  6. Identification of neoplastic cells in blood using the Sysmex XT-2000iV: a preliminary step in the diagnosis of canine leukemia.

    PubMed

    Gelain, Maria Elena; Rossi, Gabriele; Giori, Luca; Comazzi, Stefano; Paltrinieri, Saverio

    2010-06-01

    Classification of leukemias requires specialized diagnostic techniques. Automated preliminary indicators of neoplastic cells in blood would expedite selection of appropriate tests. The objective of this study was to assess the capacity of the Sysmex XT-2000iV hematology analyzer to identify neoplastic cells in canine blood samples. Blood samples (n=160) were grouped into 5 categories: acute leukemia (n=30), chronic leukemia (n=15), neoplasia without blood involvement (n=41), non-neoplastic reactive conditions (n=31), and healthy dogs (n=43). WBC counts, WBC flags, scattergrams, percentages of cells with high fluorescence intensity, and percentages of cells in the lysis-resistant region were evaluated alone or in combination to establish a "leukemic flag." Sensitivity, specificity, negative (LR-) and positive (LR+) likelihood ratios, and the number of false-negative (FN) and false-positive (FP) results were calculated, and receiver operating characteristic curves were designed for numerical values. Among single measurements and parameters, only the evaluation of scattergrams minimized FN and FP results (sensitivity 100%, specificity 94.8%, LR+ 19.17, and LR- 0.00), although their interpretation was subjective. The more objective approach based on the generation of a "leukemic flag" had a sensitivity of 100%, specificity of 87.0%, LR- of 0.00, and LR+ of 7.67. Using a novel gating strategy the Sysmex XT-2000iV may be used effectively to screen canine blood for hematopoietic neoplasia.

  7. Comparison of four hematology analyzers, CELL-DYN Sapphire, ADVIA 120, Coulter LH 750, and Sysmex XE-2100, in terms of clinical usefulness.

    PubMed

    Kang, S H; Kim, H K; Ham, C K; Lee, D S; Cho, H I

    2008-12-01

    We evaluated the clinical usefulness (leukocyte distribution classification, morphologic classification, and morphologic flags) of the following four hematology analyzers: CELL-DYN Sapphire (CD-Sapphire) (Abbott Diagnostics, Santa Clara, CA, USA), ADVIA 120 (Bayer Diagnostics, Tarrytown, NY, USA), Beckman Coulter LH 750 (Beckman Coulter, Miami, FL, USA), and Sysmex XE-2100 (TOA Medical Electronics Co., Kobe, Japan). Four hundred thirty samples from patients and 100 samples from healthy individuals were analyzed. For distributional classification, the sensitivity rates of CD-Sapphire, ADVIA 120, LH 750, and XE-2100 were 93.1, 95.9, 94.9, and 94.9%, respectively, and the efficiency rates were 80.7, 81.6, 84.1, and 84.2%, respectively. For morphologic classification, the sensitivity rates of CD-Sapphire, ADVIA 120, LH 750, and XE-2100 were 88.6, 93.2, 77.3, and 94.3%, respectively, and the efficiency rates were 80.9, 73.0, 79.5, and 74.2%, respectively. Comparing the findings in different morphologic flags, XE-2100 showed the highest sensitivity for Blasts flag (90.9%); CD-Sapphire showed the highest sensitivity for Immature granulocytes and/or Left-shift flag (85.5%); ADVIA 120 showed the highest sensitivity for Atypical lymphocytes flag (60.0%); and LH 750 showed the highest sensitivity for Nucleated RBC flag (75.0%). Our results demonstrate that the four analyzers are comparable in overall performance.

  8. 76 FR 35087 - Flag Day and National Flag Week, 2011

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-15

    ... June 15, 2011 Part II The President Proclamation 8689--Flag Day and National Flag Week, 2011 #0; #0; #0... and National Flag Week, 2011 By the President of the United States of America A Proclamation On June... Day, and during National Flag Week, we celebrate the powerful beacon of hope that our flag has become...

  9. Performance evaluation of the Abbott CELL-DYN Ruby and the Sysmex XT-2000i haematology analysers.

    PubMed

    Leers, M P G; Goertz, H; Feller, A; Hoffmann, J J M L

    2011-02-01

    Two mid-range haematology analysers (Abbott CELL-DYN Ruby and Sysmex XT-2000i) were evaluated to determine their analytical performance and workflow efficiency in the haematology laboratory. In total 418 samples were processed for determining equivalence of complete blood count (CBC) measurements, and 100 for reticulocyte comparison. Blood smears served for assessing the agreement of the differential counts. Inter-instrument agreement for most parameters was good although small numbers of discrepancies were observed. Systematic biases were found for mean cell volume, reticulocytes, platelets and mean platelet volume. CELL-DYN Ruby WBC differentials were obtained with all samples while the XT-2000i suppressed differentials partially or completely in 13 samples (3.1%). WBC subpopulation counts were otherwise in good agreement with no major outliers. Following first-pass CBC/differential analysis, 88 (21%) of XT-2000i samples required further analyser processing compared to 18 (4.3%) for the CELL-DYN Ruby. Smear referrals for suspected WBC/nucleated red blood cells and platelet abnormalities were indicated for 106 (25.4%) and 95 (22.7%) of the XT-2000i and CELL-DYN Ruby samples respectively. Flagging efficiencies for both analysers were found to be similar. The Sysmex XT-2000i and Abbott CELL-DYN Ruby analysers have broadly comparable analytical performance, but the CELL-DYN Ruby showed superior first-pass efficiency. © 2010 Blackwell Publishing Ltd.

  10. Clinical application and evaluation of Sysmex XE-5000 analyser for detecting nucleated red blood cells in peripheral blood.

    PubMed

    Zheng, Shan-Luan; Zheng, Tian; Cheng, Xiang; Hao, Xiao-Ke

    2014-01-01

    To evaluate the performance of Sysmex XE-5000 analyser for detecting nucleated red blood cells (NRBCs) in peripheral blood and investigate the clinical application of this analyser. The absolute NRBC counts (NRBC#) and percentage (NRBC%) of 137 blood specimens (NRBC-positive according to the DIFF channel of the analyser) were determined in the NRBC channel of the analyser. The intra-assay imprecision, carryover rate, and linear range of the analyser were evaluated. The NRBC% of the blood sample was detected with a microscope, and the difference between two methods was analysed. The intra-assay imprecision of the analyser for detecting NRBC# in specimens with high, moderate, and low Q-flag values were 2.10%, 3.26%, and 11.62%, respectively, and the imprecision for detecting NRBC% were 3.79%, 5.80%, and 13.33%, respectively. The carryover rates of the analyser for detecting NRBC# and NRBC% were 0.51% and 0.26%, respectively. Sysmex XE-5000 analyser had good linearity in NRBC# (i.e., 0/L to 18 x 10(9)/L). The NRBC%s of the two methods did not significantly differ (p = 0.716). The analyser can completely replace the traditional microscope for clinically classifying and counting NRBCs.

  11. Automated detection of malaria with haematology analyzer Sysmex XE-2100.

    PubMed

    Mohapatra, Sarita; Samantaray, Jyotish C; Arulselvi, S; Panda, Jitender; Munot, Khushboo; Saxena, Renu

    2011-01-01

    Diagnosis of malaria is usually made by microscopy [Giemsa, Acridine Orange (AO), and Quantitative Buffy Coat (QBC) assay], which requires expertise. Currently, automated haematology analyzers are being used for complete blood count (CBC), in all acute febrile and non-febrile illnesses which simultaneously detects malaria. The normal scattergram by the analyzer (Sysmex 2100) comprises of five parameters i.e. lymphocytes (pink), monocytes (green), neutrophils (blue), eosinophils (red) with a space between the neutrophil and eosinophil populations. We carried out a prospective study to compare the efficacy of Sysmex XE-2100 (Sysmex Corporation, Kobe) for detection of malaria in comparison to other conventional techniques. 430 cases were analyzed for malaria by microscopy (QBC, AO, Giemsa), ICT (Immunochromatography) and flowcytometric analyzer (Sysmex XE-2100). The abnormal scattergrams were observed as double neutrophil, double eosinophil, grey zone, extended neutrophil zone with a decrease space between eosinophil and neutrophil, and a combination of above patterns. Out of 70 positive cases [49/70 (70%) P. vivax, 18/70 (25.7%) P. falciparum, and 3/70 (4.2%) both P. vivax and P. falciparum], 52 showed abnormal scattergrams by the analyzer. The sensitivity and specificity of hematology analyzer found to be 74.2% and 88%, respectively. Flowcytometric analyzer is a rapid, high throughput device which needs less expertization for the diagnosis of malaria. Hence, it can be used in the diagnostic laboratories as an early modality for diagnosis of malaria in suspected as well as clinically in apparent cases.

  12. VAB Flag Painting

    NASA Image and Video Library

    2002-01-01

    On platforms suspended from the top of the 525-foot-high VAB, workers use rollers and brushes to repaint the NASA logo on the southeast side of the Vehicle Assembly Building. Known as the "meatball," the logo measures 110 feet by 132 feet, or about 12,300 square feet. The U.S. flag is also being repainted. The flag spans an area 209 feet by 110 feet, or about 23, 437 square feet. Each stripe is 9 feet wide and each star is 6 feet in diameter. The flag and logo were last painted in 1998, honoring NASA's 40th anniversary.

  13. VAB Flag Painting

    NASA Image and Video Library

    2002-01-01

    On platforms suspended from the top of the 525-foot-high VAB, workers use rollers and brushes to repaint the U.S. flag on the southwest side of the Vehicle Assembly Building. The flag spans an area 209 feet by 110 feet, or about 23, 437 square feet. Each stripe is 9 feet wide and each star is 6 feet in diameter. The logo is also being painted. Known as the "meatball," the logo measures 110 feet by 132 feet, or about 12,300 square feet. The flag and logo were last painted in 1998, honoring NASA's 40th anniversary.

  14. Analysis of reticulocyte parameters on the Sysmex XE 5000 and LH 750 analyzers in the diagnosis of inefficient erythropoiesis.

    PubMed

    Urrechaga, E; Borque, L; Escanero, J F

    2011-02-01

    The reticulocyte hemoglobin equivalent (Ret He) represents an indirect measure of the functional iron available for erythropoiesis over the previous 2-3 days. Only the analyzers of a single manufacturer, Sysmex (Sysmex Corporation, Kobe, Japan), include Ret He. Red blood cell size factor (RSf) is a new parameter provided by Beckman Coulter, which joins together the volume of the erythrocytes and the volume of reticulocytes. The aims of the study were to investigate the clinical usefulness of RSf in the study of erythropoiesis status and to assess its concordance with Ret He values. Samples from 417 patients were run on both LH 780 (Beckman Coulter) and Sysmex XE 5000 analyzers. Independent samples t-test, Pearson correlation, receiver operating characteristic (ROC) analysis and inter-rater reliability (κ index) were applied. Good correlation between RSf and Ret He was observed, r = 0.8184. Significant differences (P < 0.001) were detected when groups with inefficient erythropoiesis were compared with patients undergoing therapy and healthy subjects. ROC analysis for RSf in the diagnosis of inefficient erythropoiesis, cutoff 91.1 fl, area under curve 0.963, sensitivity 91.7%, specificity 88.5%. Concordance between RSf and Ret He κ = 0.68. RSf and Ret He are suitable parameters for the assessment of erythropoiesis status.

  15. Fragmented red cells reference range for the Sysmex XN®-series of automated blood cell counters.

    PubMed

    Lesesve, J-F; Speyer, E; Perol, J-P

    2015-10-01

    Fragmented red cells (FRCs) are a new parameter determined automatically by the latest generation of blood cell counters. FRC counts may be of interest as they may reflect schistocyte counts measured on a stained peripheral blood smear observed under the microscope. However, FRC counts depend on the technical procedure used to detect them so that reference ranges are device dependent. The XN-9000® is one of the latest models from the Sysmex series of analysers. We aimed to establish a reference range for FRCs based on 1366 normal patient samples. The mean ± SD was 0.14 ± 0.35% and the median was 0% (95% confidence interval of the mean: 0.12-0.16%). We observed that the percentage of red blood cells with <17 pg of haemoglobin content (Hypo-He) was correlated to an FRC increase and that flagged results relating to red blood cells, reticulocytes or platelets might have presented with artefactually increased FRCs. The FRCs reference range (healthy subjects) should be useful for laboratory staff for selecting which blood smears to check optically. © 2015 John Wiley & Sons Ltd.

  16. The Ultimate Flag Games.

    ERIC Educational Resources Information Center

    Angel, Kenny; Sutton, Nancy

    This paper describes six Ultimate Flag Games which offer a change from traditional games and sports that are usually geared toward athletically inclined students. These new games, aimed at middle school through college students, allow for success from the least-skilled through the most athletically talented students. Players are ability grouped…

  17. Expedition 8 Flag Raising

    NASA Image and Video Library

    2003-10-14

    Expedition 8 Commander and NASA Science Officer Michael Foale, left, and European Space Agency astronaut Pedro Duque of Spain participate in the traditional raising of the flags at the Cosmonaut Hotel, Tuesday, Oct. 14, 2003, in Baikonur, Kazakhstan. Photo Credit (NASA/Bill Ingalls)

  18. Sysmex UF-1000i performance for screening yeasts in urine.

    PubMed

    Gutiérrez-Fernández, José; Riazzo, Cristina; Sanbonmatsu, Sara; de Dios Luna, Juan; Sorlózano, Antonio; Miranda, Consuelo; Navarro, José María

    2014-04-01

    We tested the capacity of the Sysmex UF-1000i system to detect yeasts in urine by screening a total of 22 132 urine samples received for culture in our microbiology laboratory during 1 year. We also analyzed different dilutions of previously filtered urine inoculated with a strain of Candida albicans. With clinical samples, a single cut-off point of 50 yeast-like cells (YLCs)/μL detected candiduria ≥10 000 colony forming units (CFU)/mL and >100 000 CFU/mL with a sensitivity of 87.3%/95.4%, a specificity of 97%, a negative predictive value of 95.9%, and a positive predictive value of 9.3%/5.7%. With the simulated samples, a linear relationship was observed between the dilution factor and the number of cells detected by UF-1000i. This instrument appears to be able to reliably rule out candiduria of a magnitude of at least 10 000 CFU/mL and facilitate urine sample screening, thereby providing fast results. The Sysmex UF1000i system can be adapted for candiduria screening by the use of an appropriate YLCs/μL cut-off point that takes account of the prevalence of candiduria in the population.

  19. Optimal flagging combinations for best performance of five blood cell analyzers.

    PubMed

    Depoorter, M; Goletti, S; Latinne, D; Defour, Jp

    2015-02-01

    Corelab automation needs increasingly more efficient hematology analyzers and algorithms to adequately detect abnormal samples. The aim of this study is to assess the effect of combining flags or to adjust their trigger level to identify positive samples for further detection within a smear. Five hundred and seventeen EDTA samples from patients followed for hematological malignancies were randomly analyzed on Sysmex XE2100 and XN2000, Abbott Cell-Dyn Sapphire, Beckman Coulter DXH800 and Siemens ADVIA 2120. A blood smear as well as a buffy coat was further performed for each of them. Our results shows that depending on the flags, the combinations of them and the thresholds we use, analyzers can provide extremely variable results in their performances for detecting abnormal cells. ADVIA and XN2000 show remarkable performance for blasts detection. DXH800 is the most sensitive for the detection of abnormal lymphocytes, while XN outperforms the market for immature granulocytes and nucleated red blood cell. Flagging performances have been shown to be inconsistent among the different manufacturers. This article should help laboratory professionals in their quest for the best flagging schemes and give them a baseline in the selection of the most appropriate analyzer. © 2014 John Wiley & Sons Ltd.

  20. Comparison of five automated hematology analyzers in a university hospital setting: Abbott Cell-Dyn Sapphire, Beckman Coulter DxH 800, Siemens Advia 2120i, Sysmex XE-5000, and Sysmex XN-2000.

    PubMed

    Bruegel, Mathias; Nagel, Dorothea; Funk, Manuela; Fuhrmann, Petra; Zander, Johannes; Teupser, Daniel

    2015-06-01

    Various types of automated hematology analyzers are used in clinical laboratories. Here, we performed a side-by-side comparison of five current top of the range routine hematology analyzers in the setting of a university hospital central laboratory. Complete blood counts (CBC), differentials, reticulocyte and nucleated red blood cell (NRBC) counts of 349 patient samples, randomly taken out of routine diagnostics, were analyzed with Cell-Dyn Sapphire (Abbott), DxH 800 (Beckman Coulter), Advia 2120i (Siemens), XE-5000 and XN-2000 (Sysmex). Inter-instrument comparison of CBCs including reticulocyte and NRBC counts and investigation of flagging quality in relation to microscopy were performed with the complete set of samples. Inter-instrument comparison of five-part differential was performed using samples without atypical cells in blood smear (n=292). Automated five-part differentials and NRBCs were additionally compared with microscopy. The five analyzers showed a good concordance for basic blood count parameters. Correlations between instruments were less well for reticulocyte counts, NRBCs, and differentials. The poorest concordance for NRBCs with microscopy was observed for Advia 2120i (Kendall's τb=0.37). The highest flagging sensitivity for blasts was observed for XN-2000 (97% compared to 65%-76% for other analyzers), whereas overall specificity was comparable between different instruments. To the best of our knowledge, this is the most comprehensive side-by-side comparison of five current top of the range routine hematology analyzers. Variable analyzer quality and parameter specific limitations must be considered in defining laboratory algorithms in clinical practice.

  1. 77 FR 35807 - Flag Day and National Flag Week, 2012

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-15

    ...#0;#0; #0; ] Proclamation 8837 of June 11, 2012 Flag Day and National Flag Week, 2012 By the... against itself.'' This week, we mark nearly one century since that historic proclamation, and more than...), that the President annually issue a proclamation designating the week in which June 14 occurs as...

  2. 78 FR 35101 - Flag Day and National Flag Week, 2013

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-12

    ... Flag Week, 2013 By the President of the United States of America A Proclamation Each June, our Nation... responsibilities we share as citizens. This week, we celebrate that legacy, and we honor the brave men and women... President annually issue a proclamation designating the week in which June 14 occurs as ``National Flag Week...

  3. Singularity Classes of Special 2-Flags

    NASA Astrophysics Data System (ADS)

    Mormul, Piotr

    2009-11-01

    In the paper we discuss certain classes of vector distributions in the tangent bundles to manifolds, obtained by series of applications of the so-called generalized Cartan prolongations (gCp). The classical Cartan prolongations deal with rank-2 distributions and are responsible for the appearance of the Goursat distributions. Similarly, the so-called special multi-flags are generated in the result of successive applications of gCp's. Singularities of such distributions turn out to be very rich, although without functional moduli of the local classification. The paper focuses on special 2-flags, obtained by sequences of gCp's applied to rank-3 distributions. A stratification of germs of special 2-flags of all lengths into singularity classes is constructed. This stratification provides invariant geometric significance to the vast family of local polynomial pseudo-normal forms for special 2-flags introduced earlier in [Mormul P., Banach Center Publ., Vol. 65, Polish Acad. Sci., Warsaw, 2004, 157-178]. This is the main contribution of the present paper. The singularity classes endow those multi-parameter normal forms, which were obtained just as a by-product of sequences of gCp's, with a geometrical meaning.

  4. [Erythrocytic parameters Sysmex in a case of severe haemolysis].

    PubMed

    Ferrero-Vacher, Corinne; Senlis, Jean-Éric; Loustaunau, Denis; Aquaronne, Danièle; Jeandel, Pierre-Yves; Sudaka, Isabelle

    2015-01-01

    We are reporting a case of severe haemolytic anemia with cold agglutinins which combines several spurious determinations. It shows the usefulness of the new erythrocytic parameters of the XE 5000 Sysmex, specially: red blood cells with optical count (RBC-O), GR-He (intra-erythocytic hemoglobin) and R-MFV (most frequent volume). Optical red blood cells act as a substitute for red cells count instead of impedance red cells and R-MFV as a substitute for MCV (mean cell volume). The hematocrit (HCT) is corrected thanks to the following formula: HCT=(RBC-O X R- MFV)/1000. Free plasmatic hemoglobin is included in the measure of hemoglobin by the analyzer but is not available for tissue oxygenation. So, hemoglobin (HGB) has to be corrected by the means of GR- He thanks to the following formula: HGB=(GR He x RBC-O)/10.

  5. Malaria detection with the Sysmex XE-2100 hematology analyzer using pseudoeosinophilia and abnormal WBC scattergram.

    PubMed

    Huh, Hee Jin; Oh, Gwi Young; Huh, Jung Won; Chae, Seok Lae

    2008-09-01

    Recent investigation using the Sysmex XE-2100 hematology analyzer (Sysmex Corporation, Japan) has demonstrated erroneously high eosinophil counts and abnormal white blood cell (WBC) scattergrams in malaria cases. This study was conducted to assess the diagnostic efficiency of the Sysmex XE-2100 analyzer for malaria. One hundred forty-four patients initially diagnosed with Plasmodium vivax infection, 319 patients with febrile illness, and 24 patients who underwent malaria treatment were analyzed. Atypical features on Sysmex XE-2100 analyzer were categorized as pseudoeosinophilia (a gap of more than 5% in eosinophil counts between the Sysmex XE-2100 analyzer and microscopic examination) and abnormal WBC scattergram. Pseudoeosinophilia or abnormal WBC scattergram were detected in 100 of 144 malaria-positive samples (sensitivity 69.4%, specificity 100%). The samples with pseudoeosinophilia or abnormal WBC scattergrams showed significantly higher parasite counts than the samples without pseudoeosinophilia or an abnormal WBC scattergram (P<0.05). All 24 samples from patients for whom the malaria smear was repeated after malaria treatment was initiated showed a normalized eosinophil count and a normal WBC histogram. In conclusion, attention to differential count and WBC scattergrams provided by the Sysmex XE-2100 would be a valuable tool in malaria detection.

  6. First Flag on the Moon

    NASA Image and Video Library

    Four months before the launch of Apollo 11, the center director asked for ideas to celebrate the first lunar landing. Jack Kinzler answered with a suggestion to leave an American flag on the surfac...

  7. Chaotic Dynamics of Flags from Recurring Values of Flapping Moment

    NASA Astrophysics Data System (ADS)

    Virot, Emmanuel; Faranda, Davide; Amandolese, Xavier; Hémon, Pascal

    The performance of recently proposed flag-based energy harvesters is strongly limited by the chaotic response of flags to strong winds. From an experimental point of view, the detection of flag chaotic dynamics were scarce, based on the flapping amplitude and the maximal Lyapunov exponent. In practice, tracking the flapping amplitude is difficult and flawed in the large oscillation limit. Also, computing the maximal Lyapunov exponent from time series of limited size requires strong assumptions on the attractor geometry, without getting insurance of their reliability. For bypassing these issues, (1) we use a time series which takes into account the whole dynamics of the flag, by using the flapping moment which integrates its displacements, and (2) we apply an algorithm of detection of chaos based on recurring values in time series.

  8. Sysmex XT-2000iV scattergram analysis in a cat with basophilia.

    PubMed

    Stranieri, Angelica; Ferrari, Roberta; Zanzani, Sergio; Rossi, Gabriele

    2016-06-01

    A 13-year-old female Domestic Shorthair cat was presented to the Veterinary Teaching Hospital of the University of Milan for an interscapular mass suspected to be a mesenchymal malignant tumor. A preoperative CBC performed with Sysmex XT-2000iV showed leukocytosis with neutrophilia and eosinophilia. The Sysmex WBC/DIFF scattergram showed an additional, well-separated cluster of events between the neutrophil, eosinophil, and lymphocyte clusters. Blood smear evaluation revealed the presence of a significant number of basophils; thus, it was hypothesized that the additional cluster could represent the basophilic population. A second CBC, 24 days later, showed the same pattern on the WBC/DIFF scattergram in the absence of leukocytosis and neutrophilia. After surgical excision of the mass, a definitive diagnosis of feline injection site sarcoma was made. To the author's knowledge, there are no previous reports about the identification of feline basophils in the WBC/DIFF scattergram of Sysmex XT-2000iV.

  9. VLA Hosts "Flag Across America"

    NASA Astrophysics Data System (ADS)

    2001-11-01

    The National Radio Astronomy Observatory (NRAO) hosted the runners and support personnel of the "Americans United Flag Across America" run as the transcontinental memorial and fundraising effort came through New Mexico. The flag run arrived at NRAO's Very Large Array (VLA) radio telescope west of Socorro, NM, early in the post-Midnight morning of Monday, November 5, and departed after sunrise that morning en route to the Arizona border. Drivers, runners and support personnel stayed overnight at the VLA. During the night, a "VLA Night Owl Run" kept the flag moving around the VLA area until the westward trek resumed after dawn. The run began Oct. 11, one month after the terrorist attacks on New York and Washington. Organized by employees of American and United Airlines to honor the flight crews lost in those attacks, to show support for U.S. troops and to raise funds to help the victims' families, the run will take an American flag from Boston Logan Airport to Los Angeles International Airport. The Boston-to-Los Angeles trip represents the intended journey of American Flight 11 and United Flight 175, both of which were crashed by terrorists into the World Trade Center. "Our observatory was proud to host this group and honored that they brought this flag through our facility," said Miller Goss, NRAO's director of VLA operations. The runners carried a flag that flew in a U.S. F-16 over Iraq in support of Operation Southern Watch on Oct. 2, and has visited Ground Zero in Manhattan. The flag is scheduled to arrive in Los Angeles on Veterans Day, Nov. 11. The National Radio Astronomy Observatory is a facility of the National Science Foundation, operated under cooperative agreement by Associated Universities, Inc.

  10. FLAG - APOLLO XI - ASTRONAUTS - MOON

    NASA Image and Video Library

    1969-07-14

    S69-39333 (July 1969) --- This is a photographic illustration of how the flag of the United States will be implanted on the moon by the Apollo 11 astronauts. The flag is three by five feet, and is made of nylon. It will be erected on an eight-foot aluminum staff, and tubing along its top edge will unfurl it in the airless environment of the moon. The implanting of the flag is symbolic of the first time man has landed on another celestial body, and does not constitute a territorial claim by the United States. The photograph on the right shows the flag in a furled condition. Apollo 11 astronauts Neil A. Armstrong, commander; and Edwin E. Aldrin Jr., lunar module pilot, will implant the flag after their Lunar Module (LM) sets down on the moon. Astronaut Michael Collins, command module pilot, will remain with the Command and Service Modules (CSM) in lunar orbit while Armstrong and Aldrin explore the lunar surface.

  11. Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV.

    PubMed

    Oellers, Dana E; Bauer, Natali; Ginder, Melanie; Johannes, Sigrid; Pernecker, Iris; Moritz, Andreas

    2016-07-22

    Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previously (Pankraz et al., Vet Clin Path 38:30-38, 2009; Gelain et al., High fluorescent platelets fraction in macrothrombocytopenic Norfolk terrier, 2010) is largely unknown. The aim was to compare different published gates for measurement of r-PLTs with the Sysmex XT-2000iV with an own, optimized gate ("Oellers-gate") and to establish reference intervals (RIs) in > 120 dogs. Data of 362 measurements of diseased and healthy dogs were analyzed retrospectively. Several gates were applied and RIs for r-PLTs and platelet indices were established for pet dogs and a group of 153 healthy Beagles kept under defined housing conditions. Intra-assay precision (CV) was also assessed. In 30/362 samples, interferences consistent with small erythrocytes/reticulocytes were seen in the previously published gates but not in the "Oellers-gate". Good correlation was found between the different gates (rs: 0.88-1.00). RIs for the "Pankraz-gate", the "Gelain-gate", and the "Oellers-gate" were 0.0-1.2, 0.2-3.7 and 0.2-3.9 % respectively. CVs were ranging between 22 and 41 %. Optimization of previously published gates minimized interferences of small erythrocytes with r-PLT measurements.

  12. 75 FR 34309 - Flag Day and National Flag Week, 2010

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-16

    ... American flag on June 14, 1777, the thirteen stripes alternating red and white, and thirteen white stars in... configuration of stars and stripes has changed over the years it has been flown, its significance and symbolism... after Old Glory was first embraced by our Founders, the Stars and Stripes remain the symbol of...

  13. Evaluation of the Sysmex pocH-1001 haematology analyser in an outdoor oncology service.

    PubMed

    Van Hecke, I; Vanden Bempt, I; Malfait, R; Van Den Bossche, J

    2010-01-01

    Since rapid blood count analysis as near patient testing is expanding, we evaluated the use of a Sysmex pocH-100i compact haematology analyser in an outdoor oncology setting according to the recently published International Council for Standardization in Haematology (ICSH) guidelines. In total, 838 blood samples from oncology patients were analysed by pocH-100i and re-analysed by a high-throughput haematology analyser for comparison (Abbott CD-4000 or Sysmex XE-2100) to evaluate in use imprecision, comparability and vote-outs. Imprecision was less than 5%, except for platelet enumeration in the low range (within-run imprecision 7%). Good comparability was found even for platelet enumeration in the low range (r2 = 0.82). Vote-outs were found in 10.6% of examined samples. In conclusion, the Sysmex pocH-100i demonstrates good imprecision conform with former publications, produces reliable results in normal and in lower ranges comparable to the results of high throughput haematology analysers. In a well controlled management plan the Sysmex pocH-100i is suitable for near patient testing in oncology.

  14. Vertical Feature Mask Feature Classification Flag Extraction

    Atmospheric Science Data Center

    2013-03-28

    ... Feature Mask Feature Classification Flag Extraction This routine demonstrates extraction of the feature type information stored in a ... Vertical Feature Mask Feature Classification Flag Extraction routine  (5 KB) Interactive Data Language (IDL) is available from  ...

  15. Current markers of the Athlete Blood Passport do not flag microdose EPO doping.

    PubMed

    Ashenden, Michael; Gough, Clare E; Garnham, Andrew; Gore, Christopher J; Sharpe, Ken

    2011-09-01

    The Athlete Blood Passport is the most recent tool adopted by anti-doping authorities to detect athletes using performance-enhancing drugs such as recombinant human erythropoietin (rhEPO). This strategy relies on detecting abnormal variations in haematological variables caused by doping, against a background of biological and analytical variability. Ten subjects were given twice weekly intravenous injections of rhEPO for up to 12 weeks. Full blood counts were measured using a Sysmex XE-2100 automated haematology analyser, and total haemoglobin mass via a carbon monoxide rebreathing test. The sensitivity of the passport to flag abnormal deviations in blood values was evaluated using dedicated Athlete Blood Passport software. Our treatment regimen elicited a 10% increase in total haemoglobin mass equivalent to approximately two bags of reinfused blood. The passport software did not flag any subjects as being suspicious of doping whilst they were receiving rhEPO. We conclude that it is possible for athletes to use rhEPO without eliciting abnormal changes in the blood variables currently monitored by the Athlete Blood Passport.

  16. 3 CFR 8837 - Proclamation 8837 of June 11, 2012. Flag Day and National Flag Week, 2012

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... pride and all due ceremony those days from Flag Day through Independence Day, also set aside by the... 3 The President 1 2013-01-01 2013-01-01 false Proclamation 8837 of June 11, 2012. Flag Day and... June 11, 2012 Proc. 8837 Flag Day and National Flag Week, 2012By the President of the United States...

  17. The School Flag Movement: Origin and Influence.

    ERIC Educational Resources Information Center

    Sica, Morris G.

    1990-01-01

    Traces the late-nineteenth-century movement to raise the U.S. flag at public schools, Americanize immigrant students, and inspire patriotism through flag exercises and recitation of the Pledge of Allegiance. Identifies political opposition. Relates this history to the June 1989, Supreme Court decision, stating flag burning was protected under the…

  18. The School Flag Movement: Origin and Influence.

    ERIC Educational Resources Information Center

    Sica, Morris G.

    1990-01-01

    Traces the late-nineteenth-century movement to raise the U.S. flag at public schools, Americanize immigrant students, and inspire patriotism through flag exercises and recitation of the Pledge of Allegiance. Identifies political opposition. Relates this history to the June 1989, Supreme Court decision, stating flag burning was protected under the…

  19. Infrared Blobs : Time-dependent Flags

    NASA Astrophysics Data System (ADS)

    McCullough, P. R.; Mack, J.; Dulude, M.; Hilbert, B.

    2014-10-01

    We describe the creation of time-dependent flags for pixels associated with "blobs" on the WFC3 IR detector. We detect the blobs on flat fields obtained by repeated observations of the night side of the Earth. We provide the most complete census of IR blobs' positions, radii, and times of first appearance. In aggregate, a set of 46 blobs, 27 "strong" and 19 "medium" in their effective scattering cross section, affect slightly less than 1% of the pixels of the detector. A second set of 81 "weak" (and typically smaller) blobs affect another 1% of the pixels. In the past, the "blob" flag, bit 9 (i.e. value = 512) in the data quality (DQ) array described in Table 2.5 of the WFC3 Data Handbook (Rajan et al. 2010) has been a static 2-D array; henceforth a set of such arrays, each associated with a "use after" date corresponding to the appearance of one or more new blobs, can be used. We prepared such DQ arrays using the 46 "strong" and "medium" blobs and discuss why we did not include the fainter blobs therein. As an added data product, we create and test a blob flat field that corrects the effects of blobs on extended emission; however, it should not be applied if stellar photometry is the goal.

  20. Synchronized flutter of two slender flags

    NASA Astrophysics Data System (ADS)

    Mougel, Jérôme; Doaré, Olivier; Michelin, Sébastien

    2016-08-01

    The interactions and synchronization of two parallel and slender flags in a uniform axial flow are studied in the present paper by generalizing Lighthill's Elongated Body Theory (EBT) and Lighthill's Large Amplitude Elongated Body Theory (LAEBT) to account for the hydrodynamic coupling between flags. The proposed method consists in two successive steps, namely the reconstruction of the flow created by a flapping flag within the LAEBT framework and the computation of the fluid force generated by this nonuniform flow on the second flag. In the limit of slender flags in close proximity, we show that the effect of the wakes have little influence on the long time coupled-dynamics and can be neglected in the modeling. This provides a simplified framework extending LAEBT to the coupled dynamics of two flags. Using this simplified model, both linear and large amplitude results are reported to explore the selection of the flapping regime as well as the dynamical properties of two side-by-side slender flags. Hydrodynamic coupling of the two flags is observed to destabilize the flags for most parameters, and to induce a long-term synchronization of the flags, either in-phase or out-of-phase.

  1. Performance evaluation of the Sysmex Cystatin C assay on the Roche Modular P Analyzer.

    PubMed

    He, Min; Ke, Pei-Feng; Xu, Jian-Hua; Chen, Wei-Ye; Lin, Hai-Biao; Zhang, Cheng; Wang, Yun-Xiu; Zhuang, Jun-Hua; Huang, Xian-Zhang

    2016-08-01

    Serum Cystatin C (CysC) is a promising endogenous marker for estimation of glomerular filtration rate. This study evaluated the analytical performance of the Sysmex CysC assay on the Roche Modular P Chemistry Analyzer (Modular P). The evaluation of imprecision, linearity, recovery, and interference were performed in accordance with the relevant Clinical and Laboratory Standards Institute guidelines protocols, using quality controls and patient samples. Comparison studies were conducted against the Siemens and Roche assay for CysC. At CysC concentrations of 0.45 and 1.80mg/L, the within-run coefficient of variations (CVs) were 2.81% and 2.04%, respectively, and the between-run CVs were 3.14% and 3.26%. The CysC assay was proven throughout the measuring range from 0.10 to 8.00mg/L. Good correlations were achieved among the 3 CysC assays. The Sysmex assay appeared to report higher results than the Siemens assay. No interference was detected from hemoglobin 5g/L, bilirubin (free or conjugated) 200mg/L, or chyle 1500 formazin turbidity units. The Sysmex CysC assay performed well with regard to basic performance, including precision, dilution linearity, and effects of interfering substances, and is an acceptable assay for the determination of CysC on the Modular P. Copyright © 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  2. American Colleges Raise the Flag in Vietnam

    ERIC Educational Resources Information Center

    Overland, Martha Ann

    2009-01-01

    More than 30 years after the U.S. ambassador was airlifted from the embassy rooftop in Saigon with the flag tucked under his arm, a new American flag is going up in the city. This one won't be flying over the embassy. The Stars and Stripes, as well as the Texas state flag, are going up at the Saigon Institute of Technology, the only Vietnamese…

  3. American Colleges Raise the Flag in Vietnam

    ERIC Educational Resources Information Center

    Overland, Martha Ann

    2009-01-01

    More than 30 years after the U.S. ambassador was airlifted from the embassy rooftop in Saigon with the flag tucked under his arm, a new American flag is going up in the city. This one won't be flying over the embassy. The Stars and Stripes, as well as the Texas state flag, are going up at the Saigon Institute of Technology, the only Vietnamese…

  4. 3 CFR 8535 - Proclamation 8535 of June 11, 2010. Flag Day and National Flag Week, 2010

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... “a new constellation.” On Flag Day, and throughout National Flag Week, we celebrate its lasting... to people at home and around the world—as a constellation which grows brighter with every...

  5. FLAG-SGH Sedov calculations

    SciTech Connect

    Fung, Jimmy; Schofield, Sam; Shashkov, Mikhail J.

    2012-06-25

    We did not run with a 'cylindrically painted region'. However, we did compute two general variants of the original problem. Refinement studies where a single zone at each level of refinement contains the entire internal energy at t=0 or A 'finite' energy source which has the same physical dimensions as that for the 91 x 46 mesh, but consisting of increasing numbers of zones with refinement. Nominal mesh resolution: 91 x 46. Other mesh resolutions: 181 x 92 and 361 x 184. Note, not identical to the original specification. To maintain symmetry for the 'fixed' energy source, the mesh resolution was adjusted slightly. FLAG Lagrange or full (Eulerian) ALE was used with various options for each simulation. Observation - for either Lagrange or ALE, point or 'fixed' source, calculations converge on density and pressure with mesh resolution, but not energy, (not vorticity either).

  6. Image Artifact Flagging in the Kepler Pipeline

    NASA Astrophysics Data System (ADS)

    Clarke, Bruce; Caldwell, Douglas; Kolodziejczak, Jeffery

    2015-08-01

    Systematic instrument noise in the raw Kepler pixel data includes time-varying crosstalk from the fine guidance sensor (FGS) clock signals, manifestations of drifting moiré pattern as locally correlated nonstationary noise and rolling band artifacts (RBA). The Kepler pipeline module Dynablack produces best fit black level estimates per pixel per cadence by modeling a combination of collateral, FFI, reverse clocked and ARP target data. A search for transit features in the fit residuals identifies spatial regions and time intervals of strong time-varying black level which we identify as RBA. Dynablack produces flags marking these these RBA regions on a per row per cadence basis for several different transit time scales. The Photometric Analysis module (PA) generates per target per cadence data quality flags based on the Dynablack RBA flags which are in turn used by the Data Validation pipeline module (DV) in producing a contamination report associated with each target processed. The Dynablack (per row) RBA flags as well as a proxy for the per target flags produced in PA will be included in the final Kepler data release scheduled to arrive at the MAST in 2016.We detail how the RBA flags are produced in Dynablack, how these are then used to produce the per target quality flags used in DV and discuss why they should be considered when searching for transit signatures in the archived Kepler light curves. Funding for the Kepler Mission has been provided by the NASA Science Mission Directorate.

  7. 78 FR 27982 - U.S. Flag Compliance With MARPOL Annex VI International Energy Efficiency (IEE) Requirements

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-13

    ... SECURITY Coast Guard U.S. Flag Compliance With MARPOL Annex VI International Energy Efficiency (IEE... issuance of an International Energy Efficiency Certificate and the preparation of a Ship Energy Efficiency... Energy Efficiency Design Index. These requirements apply to all U.S. flag ships 400 gross tonnage...

  8. Validation of the Sysmex sp-1000i automated slide preparer-stainer in a clinical laboratory

    PubMed Central

    de Bitencourt, Eberson Damião dos Santos; Voegeli, Carlos Franco; Onzi, Gabriela dos Santos; Boscato, Sara Cardoso; Ghem, Carine; Munhoz, Terezinha

    2013-01-01

    Background The speed and quality of information have become essential items in the release of laboratory reports. The Sysmex®SP1000-I device has been developed to prepare and stain smear slides. However, for a device to be cleared for use in the laboratory routine it must pass through a validation process. Objective To evaluate the performance and reliability of the Sysmex® SP-1000i slide preparer-stainer incorporated into the routine of a hospital laboratory in Porto Alegre. Methods Peripheral blood samples of patients attending the laboratory for ambulatory exams with leukocyte counts between 7000/°L and 12,000/°L were evaluated, independent of gender and age. Two slides were prepared for each sample using the Sysmex® SP-1000i equipment; one of the slides was used to perform quality control tests using the CellaVision® DM96 device, and the other slide was used to compare pre-classification by the same device and the classification performed by a pharmacist-biochemist. Results The results of all the slides used as controls were acceptable according to the quality control test as established by the manufacturer of the device. In the comparison between the automated pre-classification and the classification made by the professional, there was an acceptable variation in the differential counts of leukocytes for 90% of the analyzed slides. Pearson correlation coefficient showed a strong correlation for band neutrophils (r = 0.802; p-value < 0.001), segmented neutrophils (r = 0.963; p-value < 0.001), eosinophils (r = 0.958; p-value < 0.001), lymphocytes (r = 0.985; p-value < 0.001) and atypical lymphocytes (r = 0.866; p-value < 0.001) using both methods. The red blood cell analysis was adequate for all slides analyzed by the equipment and by the professional. Conclusion The new Sysmex®SP1000-i methodology was found to be reliable, fast and safe for the routines of medium and large laboratories, improving the quality of microscopic analysis in complete blood

  9. Cyber Flag: A Realistic Cyberspace Training Construct

    DTIC Science & Technology

    2008-03-27

    Cyber Flag – initiating and implementing an effective , comprehensive and coordinated training environment in the relatively new, but quickly...that which is effective , comprehensive, and coordinated) is crucial to success in time of war. Red Flag iv provides critical training within the...Force Research Lab’s Human Effectiveness Division for both the support and latitude provided to me in this endeavor. The encouragement and guidance

  10. 49 CFR 393.87 - Warning flags on projecting loads.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 5 2011-10-01 2011-10-01 false Warning flags on projecting loads. 393.87 Section... ACCESSORIES NECESSARY FOR SAFE OPERATION Miscellaneous Parts and Accessories § 393.87 Warning flags on... load marked with red or orange fluorescent warning flags. Each warning flag must be at least 457 mm...

  11. 49 CFR 393.87 - Warning flags on projecting loads.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 5 2013-10-01 2013-10-01 false Warning flags on projecting loads. 393.87 Section... ACCESSORIES NECESSARY FOR SAFE OPERATION Miscellaneous Parts and Accessories § 393.87 Warning flags on... load marked with red or orange fluorescent warning flags. Each warning flag must be at least 457 mm...

  12. 49 CFR 393.87 - Warning flags on projecting loads.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Warning flags on projecting loads. 393.87 Section... ACCESSORIES NECESSARY FOR SAFE OPERATION Miscellaneous Parts and Accessories § 393.87 Warning flags on... load marked with red or orange fluorescent warning flags. Each warning flag must be at least 457 mm...

  13. 49 CFR 393.87 - Warning flags on projecting loads.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 5 2014-10-01 2014-10-01 false Warning flags on projecting loads. 393.87 Section... ACCESSORIES NECESSARY FOR SAFE OPERATION Miscellaneous Parts and Accessories § 393.87 Warning flags on... load marked with red or orange fluorescent warning flags. Each warning flag must be at least 457 mm...

  14. 49 CFR 393.87 - Warning flags on projecting loads.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 5 2012-10-01 2012-10-01 false Warning flags on projecting loads. 393.87 Section... ACCESSORIES NECESSARY FOR SAFE OPERATION Miscellaneous Parts and Accessories § 393.87 Warning flags on... load marked with red or orange fluorescent warning flags. Each warning flag must be at least 457 mm...

  15. [Evaluation of the automated coagulation analyser Sysmex(®) CS-5100 (Siemens)].

    PubMed

    Martin-Toutain, Isabelle; Jobic, Lionel; Mancic, Tessy; Brissard, Alexandre; Ankri, Annick

    2015-01-01

    The Sysmex® CS-5100 is a fully automated multiparameter hemostasis analyzer equipped with a photo-optical clot detection unit, a cap-piercing system and a pre-analytical check screens for interfering substances such as bilirubin, lipids and haemolysis (HIL system). It is designed to perform coagulation tests as well as coagulometric, chromogenic and immunologic assays. The aim of the present study was to evaluate its performance. The intra-assay and inter-assay coefficients of variation (CV) were below 6% for most parameters both in the normal and in the pathological range (exceptions: intra-assay for severe factor VIII deficiency (CV = 7.4%) and for vWFRco (CV = 7.3% and 7.7%); and inter-assay for anti-Xa activity (CV = 8.8%) and vWFRco (9.3% and 11.1%). The measured lower limits of linearity for factor VIII and factor V were satisfactory. No sample or reagent carryover was detected in the conditions of the study. Our results demonstrated that using the Sysmex® CS-5100 analyzer, routine coagulation testing can be performed with satisfactory precision. This automate has the advantage of being connectable to an automation chain.

  16. Fragmented red cells reference range (Sysmex XN(®) automated blood cell counter).

    PubMed

    Lesesve, Jean-François; Daigney, Amandine; Henry, Sylvain; Speyer, Elodie

    2015-01-01

    Fragmented red cells (FRCs) is a new parameter automatedly determined by recent blood cell counters. Their count might be of interest because FRCs are supposed to reflect schistocytes counts measured on a stained peripheral blood smear observed under the microscope. But FRCs depend from the technical procedure used to detect them and thus reference ranges are device-dependent. The XN-9000(®) is one of the last model from Sysmex series. We aimed to establish reference range for FRCs, from 2389 controls. The mean ± SD was 0.32% ± 0.81, the median 0.02% (95% confidence interval ot the mean: 0.29-0.35%). We observed that the percentage of red blood cells with less than 17 pg of hemoglobin content (Hypo-He) was correlated to FRC increase, Hypo-He increase resulting in spurious FRCs majoration. FRCs reference range should be useful for: 1) laboratory staff in order to select which blood smears to check optically; 2) Sysmex company to set-up more optimal rules proposed with the counter (automated making of blood smear).

  17. On national flags and language tags: Effects of flag-language congruency in bilingual word recognition.

    PubMed

    Grainger, Jonathan; Declerck, Mathieu; Marzouki, Yousri

    2017-07-01

    French-English bilinguals performed a generalized lexical decision experiment with mixed lists of French and English words and pseudo-words. In Experiment 1, each word/pseudo-word was superimposed on the picture of the French or UK flag, and flag-word congruency was manipulated. The flag was not informative with respect to either the lexical decision response or the language of the word. Nevertheless, lexical decisions to word stimuli were faster following the congruent flag compared with the incongruent flag, but only for French (L1) words. Experiment 2 replicated this flag-language congruency effect in a priming paradigm, where the word and pseudo-word targets followed the brief presentation of the flag prime, and this time effects were seen in both languages. We take these findings as evidence for a mechanism that automatically processes linguistic and non-linguistic information concerning the presence or not of a given language. Language membership information can then modulate lexical processing, in line with the architecture of the BIA model, but not the BIA+ model. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Evaluation of the Sysmex UF1000i flow cytometer for ruling out bacterial urinary tract infection.

    PubMed

    De Rosa, Rita; Grosso, Shamanta; Bruschetta, Graziano; Avolio, Manuela; Stano, Paola; Modolo, Maria Luisa; Camporese, Alessandro

    2010-08-05

    Urine culture is one of the most frequently requested tests in microbiology, and it represents the gold standard for the diagnosis of UTIs. Considering the high prevalence of negative results and the long TAT of the culture test, the use of a rapid and reliable screening method is becoming more and more important, as it reduces the workload, the TAT of negative results, and above all, unnecessary antibiotic prescription. The Sysmex UF1000i is a new urine flow cytometry analyzer capable of quantifying urinary particles, including BACT, WBCs, and YLCs. To evaluate the analytical performance of the UF1000i as a method for ruling out UTIs, we examined 1349 urine samples and compared the UF1000i results with standard urine culture results. With instrument cut-off values of 170BACTx10(6)/L and 150WBCsx10(6)/L, we obtained a sensitivity of 98.8%, a specificity of 76.5%, a NPV of 99.5%, and four false negative results (1.2%), avoiding the culture of 57.1% of samples. The Sysmex UF1000i was capable of improving the efficiency of a routine microbiology laboratory by processing 100samples/h and providing negative results in a few minutes, thus reducing unnecessary testing with an acceptable number of false negative results. In addition, the preliminary evaluation of B_FSC and B_FLH parameters from bacteria histograms seems to be useful for the distinction of bacterial strains detected (Gram-negatives versus Gram-positives). In fact when B_FSC was less than 30 ch, it allowed the distinction of Gram-negative bacteria in 97% of the samples. Copyright 2010 Elsevier B.V. All rights reserved.

  19. Discovering "The Italian Flag" by Fernando Melani (1907-1985)

    NASA Astrophysics Data System (ADS)

    Carlesi, Serena; Bartolozzi, Giovanni; Cucci, Costanza; Marchiafava, Veronica; Picollo, Marcello; La Nasa, Jacopo; Di Girolamo, Francesca; Dilillo, Marialaura; Modugno, Francesca; Degano, Ilaria; Colombini, Maria Perla; Legnaioli, Stefano; Lorenzetti, Giulia; Palleschi, Vincenzo

    2016-11-01

    In the occasion of the celebrations for the 150th anniversary of the founding of Italy (1861-2011), it was decided to analyse the artwork ;The Italian Flag; (La Bandiera Italiana) created by the artist Fernando Melani (Pistoia, 1907-1985), one of the precursors of the Poor Art artistic movement in Italy. This project is a follow-up to a previous study which was mainly focused on the pigments and dyes found in his home-studio. The main goal of this paper is to identify a correct diagnostic plan, based on the use of a combination of non-invasive and micro-invasive methodologies, in order to determine the state of preservation and define the best conservation procedures for a contemporary artwork. Visible, infrared and infrared false colour images as well as the Fibre Optic Reflectance Spectroscopy (FORS) technique were applied in situ to analyse The Italian Flag. Laser Induced Breakdown Spectroscopy (LIBS), Fourier Transform Infrared (FT-IR) and micro-Raman spectroscopies, Pyrolysis-Gas Chromatography/Mass Spectroscopy (Py-GC/MS), High Performance Liquid Chromatography with Diode Arrays Detection (HPLC-DAD) and Mass Spectrometric Detection (HPLC-ESI-Q-ToF) were all applied to three small samples detached from the three painted (green-blue, white and red-yellow, respectively) areas of the flag. The combination of the data obtained with all these techniques made possible a comprehensive understanding of both the chemical composition and physical behaviour of the materials used by the artist and supported curators in defining the preventive conservation of this artwork.

  20. Discovering "The Italian Flag" by Fernando Melani (1907-1985).

    PubMed

    Carlesi, Serena; Bartolozzi, Giovanni; Cucci, Costanza; Marchiafava, Veronica; Picollo, Marcello; La Nasa, Jacopo; Di Girolamo, Francesca; Dilillo, Marialaura; Modugno, Francesca; Degano, Ilaria; Colombini, Maria Perla; Legnaioli, Stefano; Lorenzetti, Giulia; Palleschi, Vincenzo

    2016-11-05

    In the occasion of the celebrations for the 150th anniversary of the founding of Italy (1861-2011), it was decided to analyse the artwork "The Italian Flag" (La Bandiera Italiana) created by the artist Fernando Melani (Pistoia, 1907-1985), one of the precursors of the Poor Art artistic movement in Italy. This project is a follow-up to a previous study which was mainly focused on the pigments and dyes found in his home-studio. The main goal of this paper is to identify a correct diagnostic plan, based on the use of a combination of non-invasive and micro-invasive methodologies, in order to determine the state of preservation and define the best conservation procedures for a contemporary artwork. Visible, infrared and infrared false colour images as well as the Fibre Optic Reflectance Spectroscopy (FORS) technique were applied in situ to analyse The Italian Flag. Laser Induced Breakdown Spectroscopy (LIBS), Fourier Transform Infrared (FT-IR) and micro-Raman spectroscopies, Pyrolysis-Gas Chromatography/Mass Spectroscopy (Py-GC/MS), High Performance Liquid Chromatography with Diode Arrays Detection (HPLC-DAD) and Mass Spectrometric Detection (HPLC-ESI-Q-ToF) were all applied to three small samples detached from the three painted (green-blue, white and red-yellow, respectively) areas of the flag. The combination of the data obtained with all these techniques made possible a comprehensive understanding of both the chemical composition and physical behaviour of the materials used by the artist and supported curators in defining the preventive conservation of this artwork. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Race Discourse and the US Confederate Flag

    ERIC Educational Resources Information Center

    Holyfield, Lori; Moltz, Matthew Ryan; Bradley, Mindy S.

    2009-01-01

    Research reveals that racial hierarchies and "color-blind" racism is maintained through discourse. The current study utilizes exploratory data from focus groups in a predominantly white southern university in the United States to examine race talk, the Confederate Flag, and the construction of southern white identity. Drawing from…

  2. The "red flags" rule in health care.

    PubMed

    Gindin, Susan E

    2009-07-01

    Important questions for hospitals to ask regarding the Federal Trade Commission's identity theft "red flags" rule include: What is the compliance deadline? Who must comply? What is required for compliance? What about HIPAA? What are the consequences of failure to comply?

  3. Solutions to the Triangular Bicycle Flags Problem

    ERIC Educational Resources Information Center

    Hartweg, Kim

    2005-01-01

    Students in a fifth-grade general education class and a second-grade gifted class participated in the Triangular Bicycle Flags problem. The results indicated that providing students with geometric experiences at the correct van Hiele level is necessary for helping students move from one level of understanding to the next.

  4. Stability and scalability of piezoelectric flags

    NASA Astrophysics Data System (ADS)

    Wang, Xiaolin; Alben, Silas; Li, Chenyang; Young, Yin Lu

    2016-02-01

    We investigate the effect of piezoelectric material on the flutter speed, vibration mode and frequency, and energy harvesting power and efficiency of a flexible flag in various fluids. We develop a fully coupled fluid-solid-electric model by combining the inviscid vortex sheet model with a linear electro-mechanical coupling model. A resistance only circuit and a resonant resistance-inductance (RL) circuit are considered. For a purely resistive circuit, an increased electro-mechanical coupling factor results in an increased flutter speed, vibration frequency, averaged electric power, and efficiency. A consistent optimal resistance is found that maximizes the flutter speed and the energy harvesting power. For a resonant RL circuit, by tuning the inductance to match the circuit frequency to the flag's vibration frequency, the flutter speed can be greatly decreased, and a larger averaged power and efficiency are obtained. We also consider a model scale setup with several commonly used commercial materials for operating in air and water. Typical ranges of dimensionless parameters are obtained for four types of material that span a wide range of solid density and rigidity values. We find that the resistance only circuit is more effective when the flag is placed in a lighter fluid (e.g., air), while the RL circuit is able to reduce the flutter speed when the flag is placed in a heavier fluid (e.g., water).

  5. Solutions to the Triangular Bicycle Flags Problem

    ERIC Educational Resources Information Center

    Hartweg, Kim

    2005-01-01

    Students in a fifth-grade general education class and a second-grade gifted class participated in the Triangular Bicycle Flags problem. The results indicated that providing students with geometric experiences at the correct van Hiele level is necessary for helping students move from one level of understanding to the next.

  6. Race Discourse and the US Confederate Flag

    ERIC Educational Resources Information Center

    Holyfield, Lori; Moltz, Matthew Ryan; Bradley, Mindy S.

    2009-01-01

    Research reveals that racial hierarchies and "color-blind" racism is maintained through discourse. The current study utilizes exploratory data from focus groups in a predominantly white southern university in the United States to examine race talk, the Confederate Flag, and the construction of southern white identity. Drawing from…

  7. What causes flag smut of wheat?

    USDA-ARS?s Scientific Manuscript database

    The causal agent of flag smut of wheat is currently subject to strict quarantine regulations in many countries and is believed to have a wide host range on wild and cultivated grasses. This fungus has been classified as both Urocystis agropyri and Urocystis tritici. Urocystis agropyri was first desc...

  8. 38 CFR 3.1607 - Cost of flags.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 3.1607 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Burial Benefits § 3.1607 Cost of flags. No reimbursement will be authorized for the cost of a burial flag... burial allowance....

  9. 38 CFR 3.1607 - Cost of flags.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 3.1607 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Burial Benefits § 3.1607 Cost of flags. No reimbursement will be authorized for the cost of a burial flag... burial allowance....

  10. 38 CFR 3.1607 - Cost of flags.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 3.1607 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Burial Benefits § 3.1607 Cost of flags. No reimbursement will be authorized for the cost of a burial flag... burial allowance....

  11. 38 CFR 3.1607 - Cost of flags.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 3.1607 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Burial Benefits § 3.1607 Cost of flags. No reimbursement will be authorized for the cost of a burial flag... burial allowance....

  12. 10 CFR 1.57 - Use of NRC flag.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... speaker's platform, the U.S. flag must occupy the position of honor and be placed at the speaker's right as he or she faces the audience, and the NRC flag must be placed at the speaker's left. ...

  13. 3 CFR 8993 - Proclamation 8993 of June 7, 2013. Flag Day and National Flag Week, 2013

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... States to display the flag during that week. NOW, THEREFORE, I, BARACK OBAMA, President of the United... of the Independence of the United States of America the two hundred and thirty-seventh.BARACK OBAMA...

  14. 14 CFR 121.557 - Emergencies: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Emergencies: Domestic and flag operations... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Operations § 121.557 Emergencies: Domestic and flag operations. (a) In an emergency situation that requires immediate decision and action...

  15. 14 CFR 121.557 - Emergencies: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Emergencies: Domestic and flag operations... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Operations § 121.557 Emergencies: Domestic and flag operations. (a) In an emergency situation that requires immediate decision and action...

  16. 7 CFR 60.130 - U.S. flagged vessel.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false U.S. flagged vessel. 60.130 Section 60.130 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... FOR FISH AND SHELLFISH General Provisions Definitions § 60.130 U.S. flagged vessel. U.S. flagged...

  17. 7 CFR 60.130 - U.S. flagged vessel.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false U.S. flagged vessel. 60.130 Section 60.130 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... FOR FISH AND SHELLFISH General Provisions Definitions § 60.130 U.S. flagged vessel. U.S. flagged...

  18. 46 CFR 154.24 - Foreign flag vessel: IMO Certificate.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Foreign flag vessel: IMO Certificate. 154.24 Section 154... SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES General § 154.24 Foreign flag... compliance with this part for the Coast Guard to endorse a foreign flag vessel's Certificate of Compliance...

  19. 38 CFR 3.1607 - Cost of flags.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Cost of flags. 3.1607 Section 3.1607 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS ADJUDICATION Burial Benefits § 3.1607 Cost of flags. No reimbursement will be authorized for the cost of a burial flag...

  20. 10 CFR 1002.22 - Use of distinguishing flag.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Use of distinguishing flag. 1002.22 Section 1002.22 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Distinguishing Flag § 1002.22... Department auditoriums, official presentation rooms, hearing rooms, lobbies, public document rooms, and...

  1. 7 CFR 60.130 - U.S. flagged vessel.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false U.S. flagged vessel. 60.130 Section 60.130 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards... FOR FISH AND SHELLFISH General Provisions Definitions § 60.130 U.S. flagged vessel. U.S. flagged...

  2. 10 CFR 1002.22 - Use of distinguishing flag.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Secretarial officers, Chairman of the Federal Energy Regulatory Commission, and heads of field locations... 10 Energy 4 2011-01-01 2011-01-01 false Use of distinguishing flag. 1002.22 Section 1002.22 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Distinguishing Flag § 1002.22...

  3. 10 CFR 1002.22 - Use of distinguishing flag.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Secretarial officers, Chairman of the Federal Energy Regulatory Commission, and heads of field locations... 10 Energy 4 2012-01-01 2012-01-01 false Use of distinguishing flag. 1002.22 Section 1002.22 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Distinguishing Flag § 1002.22...

  4. 10 CFR 1002.22 - Use of distinguishing flag.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Secretarial officers, Chairman of the Federal Energy Regulatory Commission, and heads of field locations... 10 Energy 4 2014-01-01 2014-01-01 false Use of distinguishing flag. 1002.22 Section 1002.22 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Distinguishing Flag § 1002.22...

  5. 10 CFR 1002.22 - Use of distinguishing flag.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Secretarial officers, Chairman of the Federal Energy Regulatory Commission, and heads of field locations... 10 Energy 4 2013-01-01 2013-01-01 false Use of distinguishing flag. 1002.22 Section 1002.22 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Distinguishing Flag § 1002.22...

  6. 14 CFR 121.595 - Dispatching authority: Flag operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Dispatching authority: Flag operations. 121... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.595 Dispatching authority: Flag operations. (a) No person may start a flight unless an...

  7. 14 CFR 121.595 - Dispatching authority: Flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Dispatching authority: Flag operations. 121... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.595 Dispatching authority: Flag operations. (a) No person may start a flight unless an...

  8. 14 CFR 121.595 - Dispatching authority: Flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Dispatching authority: Flag operations. 121... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.595 Dispatching authority: Flag operations. (a) No person may start a flight unless an...

  9. 14 CFR 121.595 - Dispatching authority: Flag operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Dispatching authority: Flag operations. 121... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.595 Dispatching authority: Flag operations. (a) No person may start a flight unless an...

  10. 14 CFR 121.595 - Dispatching authority: Flag operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Dispatching authority: Flag operations. 121... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.595 Dispatching authority: Flag operations. (a) No person may start a flight unless an...

  11. New parameters available on Sysmex XE-5000 hematology analyzers contribute to differentiating dengue from leptospirosis and enteric fever.

    PubMed

    Oehadian, A; Michels, M; de Mast, Q; Prihatni, D; Puspita, M; Hartantri, Y; Sinarta, S; van der Ven, A J A M; Alisjahbana, B

    2015-12-01

    Distinguishing dengue virus infection from other febrile thrombocytopenic illnesses such as leptospirosis or enteric fever is important but difficult, due to the unavailability of reliable diagnostic tests. Sysmex XE-5000 hematology analyzers use fluorescence flow cytometry to quantitate new parameters including cells in the atypical lymphocyte area (AL), high-fluorescent lymphocyte counts (HFLC), immature granulocytes (IG), and immature platelets (IPF). This study aimed to investigate whether these parameters can help to discriminate between the diseases. We compared hematocytometry performed by a Sysmex XE-5000 analyzer in Indonesian adults with dengue (n = 93), leptospirosis (n = 11), and enteric fever (n = 6) infection, and in healthy controls (n = 28). Receiver operating characteristic curves comparing dengue and leptospirosis showed that dengue was characterized by increased %AL (AUC 0.87; 95% CI 0.70-1.03), %HFLC (AUC 0.89; 95% CI 0.78-0.99), and %IPF (AUC 0.81; 95% CI 0.65-0.97), while patients with leptospirosis had increased %IG (AUC 0.86; 95% CI 0.71-1.02). Low %AL, %HFLC, and %IG supported a diagnosis of enteric fever. The detection of AL, HFLC, IG, and IPF by Sysmex XE-5000 hematology analyzers can help to differentiate between common causes of febrile illnesses with thrombocytopenia in dengue endemic areas. We recommend further investigating the discriminatory value of these parameters in clinical practice. © 2015 John Wiley & Sons Ltd.

  12. Divergent Receiver Responses to Components of Multimodal Signals in Two Foot-Flagging Frog Species

    PubMed Central

    Preininger, Doris; Boeckle, Markus; Sztatecsny, Marc; Hödl, Walter

    2013-01-01

    Multimodal communication of acoustic and visual signals serves a vital role in the mating system of anuran amphibians. To understand signal evolution and function in multimodal signal design it is critical to test receiver responses to unimodal signal components versus multimodal composite signals. We investigated two anuran species displaying a conspicuous foot-flagging behavior in addition to or in combination with advertisement calls while announcing their signaling sites to conspecifics. To investigate the conspicuousness of the foot-flagging signals, we measured and compared spectral reflectance of foot webbings of Micrixalus saxicola and Staurois parvus using a spectrophotometer. We performed behavioral field experiments using a model frog including an extendable leg combined with acoustic playbacks to test receiver responses to acoustic, visual and combined audio-visual stimuli. Our results indicated that the foot webbings of S. parvus achieved a 13 times higher contrast against their visual background than feet of M. saxicola. The main response to all experimental stimuli in S. parvus was foot flagging, whereas M. saxicola responded primarily with calls but never foot flagged. Together these across-species differences suggest that in S. parvus foot-flagging behavior is applied as a salient and frequently used communicative signal during agonistic behavior, whereas we propose it constitutes an evolutionary nascent state in ritualization of the current fighting behavior in M. saxicola. PMID:23383168

  13. Modeling Classical Heat Conduction in FLAG

    SciTech Connect

    Ramsey, Scott D.; Hendon, Raymond Cori

    2015-01-12

    The Los Alamos National Laboratory FLAG code contains both electron and ion heat conduction modules; these have been constructed to be directly relevant to user application problems. However, formal code verification of these modules requires quantitative comparison to exact solutions of the underlying mathematical models. A wide variety of exact solutions to the classical heat conduction equation are available for this purpose. This report summarizes efforts involving the representation of the classical heat conduction equation as following from the large electron-ion coupling limit of the electron and ion 3T temperature equations, subject to electron and ion conduction processes. In FLAG, this limiting behavior is quantitatively verified using a simple exact solution of the classical heat conduction equation. For this test problem, both heat conduction modules produce nearly identical spatial electron and ion temperature profiles that converge at slightly less than 2nd order to the corresponding exact solution.

  14. Infinite flag varieties and conjugacy theorems

    PubMed Central

    Peterson, Dale H.; Kac, Victor G.

    1983-01-01

    We study the orbit of a highest-weight vector in an integrable highest-weight module of the group G associated to a Kac-Moody algebra [unk](A). We obtain applications to the geometric structure of the associated flag varieties and to the algebraic structure of [unk](A). In particular, we prove conjugacy theorems for Cartan and Borel subalgebras of [unk](A), so that the Cartan matrix A is an invariant of [unk](A). PMID:16593298

  15. Usefulness of thresholds for smear review of neutropenic samples analyzed with a Sysmex XN-10 analyzer.

    PubMed

    Ronez, Emily; Geara, Carole; Coito, Sylvie; Jacqmin, Hugues; Cornet, Edouard; Troussard, Xavier; Chatelain, Bernard; Mullier, François

    2017-10-01

    Neutropenia is one of the main criteria for a blood smear review. The objective of this study was to compare the thresholds proposed by the international consensus group for hematology review (1.0 10(9)/L) and the French speaking Group for Cellular Haematology (1.5 10(9)/L) in terms of the number of useless smears. We collected 112,097 analyzed samples from four laboratories equipped with XN instruments (Sysmex, Kobe, Japan) during early 2016. The only exclusion criterion was a leucocyte count below 0.5 10(9)/L. In the absence of abnormal cells and/or morphology suggesting haematological disease, samples were classified as 'negative for morphology' and the differential from the XN-10 was reported. These smear procedures were considered as uninformative. Some 2202 samples met the criterion for neutropenia (<1.5 10(9)/L) for slide review representing 1.96% of the total. These included 1031 with neutropenia alone and 1171 neutropenia plus other abnormalities. Of the 1031 with neutropenia alone, 886 had a neutrophil count between 1.0 10(9)/L and 1.5 10(9)/L. The smear was uninformative for all of these samples. In conclusion, microscopic examination of a blood smear provided very limited information in cases of neutropenia without other abnormalities.

  16. Performance evaluation of the automated nucleated red blood cell enumeration on Sysmex XN analyser.

    PubMed

    Tantanate, C; Klinbua, C

    2015-06-01

    Presence of peripheral blood nucleated red blood cell (NRBC) is associated with pathological conditions and leads to the overestimation of white blood cell count in automated haematology analysers (HA). The authors evaluated NRBC enumeration by a new HA Sysmex XN (XN) to demonstrate the precision and comparability to manual count (MC) at the various NRBC values. Specimens with initially NRBC positive were included. For precision assessment, 8 levels of NRBCs were repeatedly analysed. For comparison study, 234 specimens were analysed by both XN and MC. For precision study, the percentage of coefficient of variation ranged from 14% to 45.6% and 1.2% to 4.4% for MC and XN, respectively. For comparison study between XN and MC, NRBCs ranged from 0% to 612.5%. Regression analysis demonstrated an r(2) of 0.98. The mean bias of 14.1% with 95% limits of agreement between -48.76% and 76.95% was found. The NRBC counts from XN appeared to be more in accordance with MC when the NRBCs were lower than 200% with the concordance rate of 94.2%. The automated NRBC enumeration by XN was precise and could replace the traditional MC, especially for the specimens with NRBCs lower than 200%. © 2014 John Wiley & Sons Ltd.

  17. Sample stability for complete blood cell count using the Sysmex XN haematological analyser

    PubMed Central

    Daves, Massimo; Zagler, Elmar M.; Cemin, Roberto; Gnech, Flora; Joos, Alexandra; Platzgummer, Stefan; Lippi, Giuseppe

    2015-01-01

    Background Sample stability is a crucial aspect for the quality of results of a haematology laboratory. This study was conducted to investigate the reliability of haematological testing using Sysmex XN in samples stored for up to 24 h at different temperatures. Materials and methods Haematological tests were performed on whole blood samples collected from 16 ostensibly healthy outpatients immediately after collection and 3 h, 6 h or 24 h afterwards, with triple aliquots kept at room temperature, 4 °C or 37 °C. Results No meaningful bias was observed after 3 h under different storage conditions, except for red blood cell distribution width (RDW) and platelet count (impedance technique, PLT-I) at 37 °C. After 6 h, meaningful bias was observed for mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) at room temperature, red blood cell (RBC) count, mean corpuscular haemoglobin concentration (MCHC), MCH, MCV and PLT-I at 4 °C, and RBC, RDW, MCHC, MCH and PLT-I at 37 °C. After 24 h, a meaningful bias was observed for MCHC, MCV, platelet count (fluorescent technique, PLT-F) and mean platelet volume (MPV) at room temperature, MCHC, MCV, PLT-I and MPV at 4 °C, and all parameters except RBC count and MPV at 37 °C. Discussion Great caution should be observed when analysing results of haematological tests conducted more than 3 h after sample collection. PMID:26057491

  18. Cell analysis in cerebrospinal fluid (CSF) using Sysmex® hematology analyzers XT-4000i and XE-5000: evaluation with CSF controls of the Joint German Society for Clinical Chemistry and Laboratory Medicine (DGKL).

    PubMed

    Kleine, Tilmann O; Nebe, Carl Thomas; Löwer, Christa; Geilenkeuser, Wolf-Jochen; Dorn-Beineke, Alexandra

    2012-03-01

    In cerebrospinal fluid (CSF) analysis, hematology analyzers (HAs) Sysmex® XT-4000i and XE-5000, equipped with flow cytometry (FCM), were used to count cells and differentiate leukocytes into mononuclear and polymorphonuclear cells (MNCs, PMCs) applying body fluid mode. FCM was evaluated with 20 DGKL CSF controls containing viable human leukocytes and erythrocytes. HA values were compared with reference values by Passing/Bablok regression analysis to reveal conformity. Conformity of white blood cells (WBCs) was obtained with native leukocytes, counted in calibrated Fuchs-Rosenthal chamber as reference; red blood cell counts proved inaccurate. CV <40% with WBC counts <20 per μL impairs accuracy. Reference WBC differentiation was assayed using FACS Canto II™ and FC-500 SN with anti-CD45, anti-CD14, anti-CD16, anti-CD16/56 [Becton Dickinson (BD); Beckman Coulter (BC)]. BD FACS lysing solution®-no-wash-procedure was applied. BC pretreatment with Versalyse lysing solution was not recommended. MNCs (lymphocytes + monocytes) were significantly lower (∼14%) on both HAs; PMCs (granulocytes or sum of neutrophils + eosinophils + basophils: range 1-86 M/L) were significantly higher (∼2.2-fold). WBC HA differentiation is not reliable because MNC/PMC differentiation yielded lower and higher values than FACS-FCM references, respectively. This is attributed to incorrect discrimination of leukocytes with rounded/nonrounded nuclei; adding leukocytes with nonrounded nuclei to too low HA MNCs (about 40% not-activated) yielded P/B conformity; subtraction of leukocytes with nonrounded nuclei from elevated HA PMCs showed conformity (about 85% activated). Nucleus/activation state of leukocytes was assessed using microhistology. Sysmex XT-4000i and XE-5000 HAs systems are inappropriate for complete CSF cell analysis. Copyright © 2012 International Society for Advancement of Cytometry.

  19. Performance evaluation of the Sysmex XE-5000 hematology analyzer for white blood cell analysis in cerebrospinal fluid.

    PubMed

    Perné, Andrea; Hainfellner, Johannes A; Womastek, Irene; Haushofer, Alexander; Szekeres, Thomas; Schwarzinger, Ilse

    2012-02-01

    The newest generation hematology analyzer, Sysmex XE-5000 (Sysmex Corporation, Kobe, Japan) is equipped with an improved body fluid analysis mode. To evaluate the applicability of the XE-5000 analyzer to white blood cell (WBC) analysis in cerebrospinal fluid (CSF). A total of 425 routinely collected, consecutive CSF samples were included in the study. For a comparison of total WBC counts, the results of routine chamber counts were grouped into categories of 0 to 5 (n  =  330), >5 to 10 (n  =  36), >10 to 50 (n  =  39), >50 to 200 (n  =  15), and >200 (n  =  5) WBC/µL. Microscopic differential counts were performed using cytospins from 276 samples. Results were grouped according to the percent content of polymorphonuclear (PMN) cells, 0% to 25% (n  =  263), >25% to 50% (n  =  7), >50% to 75% (n  =  3), and >75% to 100% (n  =  3) of WBC. Corresponding results of XE-5000 analysis were matched to these particular count categories. For total WBC counts, the proportions of samples correctly classified by the XE-5000 from the percentage groups described above were 88%, 47%, 72%, 93%, and 100%, respectively. After the two lowest count categories were combined into one range of 0 to 10 WBC/µL, matches increased to 95%. For PMN counts in the 0% to 25% group, 37% of samples were misclassified by the XE-5000. Conversely, for samples with microscopic PMN counts of more than 25%, there was a trend toward underestimation by the XE-5000. Mismatches were most pronounced in samples with fewer than 10 WBC/µL. The Sysmex XE-5000 hematology analyzer yields valid total CSF cell counts and may be considered an acceptable alternative to the traditional chamber method, even for samples with low WBC counts. However, it cannot be recommended as a suitable alternative for manual differential cytologic workup.

  20. Hypo- and hypernatremia results in inaccurate erythrocyte mean corpuscular volume measurement in vitro, when using Sysmex XE 2100.

    PubMed

    Philipsen, Jens Peter; Madsen, Kirsten Vikkelsø

    2015-11-01

    Automated hematology analyzers dilute patient erythrocytes with an isoosmotic diluent before quantitating the erythrocyte mean cell volume (MCV). However, if patient plasma osmolality differs from the diluent, water will cross the erythrocytes membrane and establish a new equilibrium across the membrane. Since the new equilibrium is reached before the measurement of the MCV, the measured MCV may not reflect the true MCV in vivo. Calculation of the theoretical change in MCV at changed P-Sodium/P-Osmolality and to investigate if the automated blood cell counter Sysmex XE 2100 measures MCV correctly in hypo- and hyperosmolality and hypo-and hypernatremia. In addition, to examine whether the theoretically calculated change in MCV corresponds with the experimentally determined MCV change. Theoretical calculation of the MCV inaccuracy at hypo- and hypernatremia, as well as at hypo- and hyperosmolality. Experimental studies with comparison of MCV measured at Sysmex XE 2100 to MCV found by using the manual measured packed cell volume method. Measurement of MCV in hypo- and hypernatremia patients using the automated blood cell counter Sysmex XE 2100 resulted in inaccurate MCV. The experimental results also revealed a strong correlation between P-Osmolality/P-Sodium and MCV inaccuracy (R(2) = 0.70/0.85) similar to the theoretically calculated MCV inaccuracy. We suggest using mean cellular Hb (MCH) instead of MCV, mean corpuscular Hb concentration (MCHC) and B-Erythrocyte volume fraction (EVF). Alternatively, we suggest standardizing the measured MCV to a normal P-Sodium e.g. 140 mmol/L to estimate the in vivo MCV.

  1. Comparison of the Sysmex XT-2000iV with microscopic differential counts of canine bone marrow.

    PubMed

    Pernecker, Iris; Bauer, Natali B; Johannes, Sigrid; Ginder, Melanie; Harleman, Johannes H; Moritz, Andreas

    2017-03-01

    Canine bone marrow is frequently assessed in the advanced preclinical research environment. Automated analysis provides time savings and objectivity over the gold standard of microscopic (cytologic) evaluation. We compared the analysis of 90 canine bone marrow samples by the Sysmex XT-2000iV hematology analyzer (Sysmex Corp., Kobe, Japan) with cytologic evaluation. Gates for cell populations were created in the system's WBC/BASO channel. Variables "total nucleated red blood cells" (total_NRBC), "poly- and orthochromatic nucleated red blood cells" (poly_orth_NRBC), "total neutrophils" (total_NEUT), "mature neutrophils" (mature_NEUT), and myeloid-to-erythroid (M:E) ratio were compared with cytologic evaluation. Intra-assay repeatability and total error (TE) were calculated for both methods. Intra-assay repeatability was 0.95-2.48% for the XT-2000iV and 8.32-23.23% for cytology. Observed TE for the automated measurement was 5.16-46.8% and for cytology 22.70-76.74%. Spearman rank correlation was excellent for M:E ratio (0.91) and fair for the other populations (0.65-0.71). Absolute bias for M:E ratio was low (-0.114). A negative absolute bias of -7.71% for the XT-2000iV was found for poly_orth_NRBC, whereas the bias was positive for total_NEUT (7.10%) and mature_NEUT (14.67%). M:E ratio of canine bone marrow samples can be precisely determined using the Sysmex XT-2000iV WBC/BASO channel. Total_NRBC, poly_orth_NRBC, total_NEUT, and mature_NEUT can be estimated rapidly. With distinctly lower coefficient of variation and observed TE compared with cytology, automated measurement provides advantages in terms of standardization, and it is suited to the advanced preclinical research environment where large numbers of samples are investigated.

  2. Red flags to screen for vertebral fracture in patients presenting with low-back pain.

    PubMed

    Williams, Christopher M; Henschke, Nicholas; Maher, Christopher G; van Tulder, Maurits W; Koes, Bart W; Macaskill, Petra; Irwig, Les

    2013-01-31

    Low-back pain (LBP) is a common condition seen in primary care. A principal aim during a clinical examination is to identify patients with a higher likelihood of underlying serious pathology, such as vertebral fracture, who may require additional investigation and specific treatment. All 'evidence-based' clinical practice guidelines recommend the use of red flags to screen for serious causes of back pain. However, it remains unclear if the diagnostic accuracy of red flags is sufficient to support this recommendation. To assess the diagnostic accuracy of red flags obtained in a clinical history or physical examination to screen for vertebral fracture in patients presenting with LBP. Electronic databases were searched for primary studies between the earliest date and 7 March 2012. Forward and backward citation searching of eligible studies was also conducted. Studies were considered if they compared the results of any aspect of the history or test conducted in the physical examination of patients presenting for LBP or examination of the lumbar spine, with a reference standard (diagnostic imaging). The selection criteria were independently applied by two review authors. Three review authors independently conducted 'Risk of bias' assessment and data extraction. Risk of bias was assessed using the 11-item QUADAS tool. Characteristics of studies, patients, index tests and reference standards were extracted. Where available, raw data were used to calculate sensitivity and specificity with 95% confidence intervals (CI). Due to the heterogeneity of studies and tests, statistical pooling was not appropriate and the analysis for the review was descriptive only. Likelihood ratios for each test were calculated and used as an indication of clinical usefulness. Eight studies set in primary (four), secondary (one) and tertiary care (accident and emergency = three) were included in the review. Overall, the risk of bias of studies was moderate with high risk of selection and

  3. Physicians of the Bear-Flag Republic.

    PubMed

    Bengtsson, Bengt-Ola S

    2016-11-01

    In the mid-nineteenth century, three events coincided to make California the 31st state of the USA: The Mexican-American war of 1846-1848, The Bear Flag Revolt of 1846 and the discovery of gold in 1848. Within a few years a population explosion had occurred and with it came the need for organization of the practice of medicine. This paper outlines the biographies of some physicians who were instrumental in the early years of the Golden State. © IMechE 2015.

  4. Red flag imaging techniques in Barrett's esophagus.

    PubMed

    Saxena, Payal; Canto, Marcia Irene

    2013-07-01

    The key to detection and treatment of early neoplasia in Barrett's esophagus (BE) is thorough and careful inspection of the Barrett's segment. The greatest role for red flag techniques is to help identify neoplastic lesions for targeted biopsy and therapy. High-definition white light endoscopy (HD-WLE) can potentially improve endoscopic imaging of BE compared with standard endoscopy, but little scientific evidence supports this. The addition of autofluorescence imaging to HD-WLE and narrow band imaging increases sensitivity and the false-positive rate without significantly improving overall detection of BE-related neoplasia.

  5. 3 CFR 8391 - Proclamation 8391 of June 11, 2009. Flag Day and National Flag Week, 2009

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... “thirteen stripes, alternate red and white; that the union be thirteen stars, white in a blue field... billowing Stars and Stripes. And each day as the flag is raised above military installations and government buildings, we are reminded of the great sacrifices that have been made in defense of our Nation. The...

  6. The Flag of the United States and State Flags, Seals & Mottoes.

    ERIC Educational Resources Information Center

    Kennon, Donald R.; Kerr, Mary Lee

    A detailed and descriptive narrative is presented about the U.S. flag's history, development, symbolic meaning, and overall importance. Information about the U.S. seal and how it identifies what the United States stands for as a nation is given. A motto is defined as a word or phrase that expresses one's principles, religious, moral, or patriotic…

  7. The Flag of the United States and State Flags, Seals & Mottoes.

    ERIC Educational Resources Information Center

    Kennon, Donald R.; Kerr, Mary Lee

    A detailed and descriptive narrative is presented about the U.S. flag's history, development, symbolic meaning, and overall importance. Information about the U.S. seal and how it identifies what the United States stands for as a nation is given. A motto is defined as a word or phrase that expresses one's principles, religious, moral, or patriotic…

  8. Interaction between two side-by-side inverted flags

    NASA Astrophysics Data System (ADS)

    Huertas-Cerdeira, Cecilia; Fan, Boyu; Barizien, Antoine; Gharib, Morteza

    2015-11-01

    The inverted flag instability occurs when an elastic plate that is free at its leading edge and clamped at its trailing edge is subjected to an axial wind. The oscillating motion that follows has received recent attention. However, previous studies have focused on the dynamics of a single flag even though these are rarely found isolated in natural phenomena, such as the fluttering of leaves in the wind. The interaction between two side-by-side inverted flags has been investigated, analyzing the effects of the distance between flags and the wind speed. Both in-phase and anti-phase coupling have been observed for different ranges of these parameters.

  9. Construction of FLAG tagging vectors for Candida albicans.

    PubMed

    Umeyama, Takashi; Nagai, Yuki; Niimi, Masakazu; Uehara, Yoshimasa

    2002-05-01

    We have constructed three new vectors for Candida albicans (pFLAG-Act1, pFLAG-Mal2, and pFLAG-Met3). The proteins can be expressed as C-terminal FLAG-tagged proteins under the control of different promoters (ACT1, MAL2, and MET3). To confirm the protein expression, we used the Renilla reniformis luciferase and the drug efflux pump Cdr1p of Candida albicans as reporters. The luciferase protein expressed by the MET3 promoter was found to have the strongest activity of the three promoters when cultured in a methionine-depleted synthetic medium. Cdr1p was expressed as a C-terminal FLAG-tagged protein using either these vectors or PCR-mediated integration. The fluconazole resistance was increased by the Cdr1p expression in a CDR1 homozygous disruptant. The expressed proteins were detected by Western blotting using the anti-FLAG antibody. We also constructed a Cdr1p-FLAG expressing strain, in which we directly tagged Cdr1p with FLAG on the genome loci, using a PCR-based integrative marker cassette that was amplified using the pFLAG vector. We then confirmed the protein expression by Western blotting. Thus, these new vectors are useful as C. albicans genetic tools. Copyright 2002 John Wiley & Sons, Ltd.

  10. 36. FLAG TOWER CLOCK ZONE FROM SOUTH TOWER ROOF, LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. FLAG TOWER CLOCK ZONE FROM SOUTH TOWER ROOF, LOOKING NORTH - Smithsonian Institution Building, 1000 Jefferson Drive, between Ninth & Twelfth Streets, Southwest, Washington, District of Columbia, DC

  11. 19. NORTH TOWER, PORTE COCHERE & FLAG TOWER, LOOKING SOUTHWEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. NORTH TOWER, PORTE COCHERE & FLAG TOWER, LOOKING SOUTHWEST - Smithsonian Institution Building, 1000 Jefferson Drive, between Ninth & Twelfth Streets, Southwest, Washington, District of Columbia, DC

  12. 18. NORTH TOWER, PORTE COCHERE & FLAG TOWER, LOOKING SOUTHWEST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. NORTH TOWER, PORTE COCHERE & FLAG TOWER, LOOKING SOUTHWEST BY WEST - Smithsonian Institution Building, 1000 Jefferson Drive, between Ninth & Twelfth Streets, Southwest, Washington, District of Columbia, DC

  13. Identification of spurious hemolysis in anticoagulated blood with Sysmex XE-2100 and Siemens Advia 2120.

    PubMed

    Lippi, Giuseppe; Pipitone, Silvia; Gennari, Daniela; Franchini, Massimo

    2012-01-01

    Various degrees of hemolysis might occur during collection, processing and storage of blood bags or blood tubes for hematological testing. In both circumstances, the identification of hemolysis is challenging since the centrifugation process is not required. The aim of this study was to identify simple hematological parameters that would help identify the presence of hemolysis in anticoagulated blood. Twenty tubes containing K2EDTA anticoagulated blood were randomly selected from outpatient samples and divided in two aliquots. The former was immediately analyzed, whereas the latter was subjected to mechanical hemolysis by aspirating whole blood two times through a very fine needle to generate mechanical hemolysis. Both aliquots were tested on Advia 2120 and Sysmex XE-2100. The double aspiration of the blood through the fine needle caused a remarkable hemolysis with significant decrease of red blood cell (RBC) count (-17 +/- 11%; p < 0.01), hematocrit (-18 +/- 12%; p < 0.01) and reticulocytes (-24 +/- 13%; p < 0.01), but not of hemoglobin, white blood cell or platelet counts. A remarkable increase of immature platelet fraction (IPF) on XE-2100 and RBC ghosts on Advia 2120 was observed in the hemolyzed samples, whereas RBC fragments did not vary significantly. A significant correlation was also observed between hemolysis and reticulocyte count (r = 0.823; p < 0.01), IPF (r = -0.502, p = 0.024) and RBC ghosts (r = -0.711; p < 0.01). Receiver Operating Characteristic (ROC) curve analysis demonstrated a good performance of both IPF and RBC ghosts (i.e., AUCs 0.91 and 0.96, respectively) for distinguishing non-hemolyzed from hemolyzed specimens. The absolute values of both IPF and RBC ghosts perform efficiently for distinguishing hemolyzed from non-hemolyzed specimens, although neither reached 100% sensitivity and specificity. Nevertheless, the demonstration that both parameters significantly increase after hemolysis can be reliably used to distinguish hemolyzed from non

  14. Differential signalling of NH2-terminal flag-labelled thrombopoietin receptor activated by TPO or anti-FLAG antibodies.

    PubMed

    Millot, Gaël A; Vainchenker, William; Duménil, Dominique; Svinarchuk, Fédor

    2004-03-01

    In this report, we compared activation of NH2-terminal FLAG-labelled thrombopoietin receptor (Mpl) by anti-FLAG antibodies and by thrombopoietin (TPO). We found that anti-FLAG monoclonal antibodies M1 dimerize FLAG-labelled receptor and trigger proliferation of BaF3/FLAG-Mpl cells. In UT7/FLAG-Mpl cells, activation of the FLAG-Mpl receptor by low TPO concentrations triggered proliferation, while high concentrations triggered differentiation. Activation of FLAG-Mpl receptors in these cells by all tested concentrations of M1 antibodies induced proliferation but not differentiation. Low TPO concentrations induced similar to M1 antibodies level of Jak2, Stat3, Stat5 and Akt phosphorylation. In contrast, only TPO and not M1 antibodies activated Erks phosphorylation. Since the anti-FLAG antibodies do not react with the TPO binding site of the receptor, we hypothesize that they can trigger a distinct signal by dimerizing Mpl in a manner different from that induced by TPO.

  15. Where no flag has gone before: Political and technical aspects of placing a flag on the Moon

    NASA Technical Reports Server (NTRS)

    Platoff, Anne M.

    1993-01-01

    The flag on the Moon represents an important event in vexillological history. The political and technical aspects of placing a flag on the Moon, focusing on the first Moon landing, is examined. During their historic extravehicular activity, the Apollo 11 crew planted the flag of the United States on the lunar surface. This flag-raising was strictly a symbolic activity, as the United Nations Treaty on Outer Space precluded any territorial claim. Nevertheless, there were domestic and international debates over the appropriateness of the event. Congress amended the agency's appropriations bill to prevent the National Aeronautics and Space Administration (NASA) from placing flags of other nations, or those of international associations, on the Moon during missions funded solely by the United States. Like any activity in space exploration, the Apollo flag-raising also provided NASA engineers with an interesting technical challenge. They designed a flagpole with a horizontal bar allowing the flag to 'fly' without the benefit of wind to overcome the effects of the Moon's lack of an atmosphere. Other factors considered in the design were weight, heat resistance, and ease of assembly by astronauts whose space suits restricted their range of movement and ability to grasp items. As NASA plans a return to the Moon and an expedition to Mars, we will likely see flags continue to go 'where no flag has gone before'.

  16. Use of Sysmex XE-2100 and XE-5000 hematology analyzers for the diagnosis of malaria in a nonendemic country (France).

    PubMed

    Dubreuil, P; Pihet, M; Cau, S; Croquefer, S; Deguigne, P A; Godon, A; Genevieve, F; De Gentile, L; Zandecki, M

    2014-04-01

    Most studies dealing with automated hematology analyzers (HAs) and malaria diagnosis are conducted in endemic countries. We retrospectively studied cell blood counts (CBCs) performed with Sysmex XE-2100 and XE-5000 HAs in our center (Angers, France) regarding 67 patients returning from endemic areas and infected with various Plasmodium species. In 83% of infected samples with Plasmodium vivax (Pv), ovale (Po), or malariae (Pm), extra clouds of dots were present in neutrophil and/or eosinophil area(s) on routine differential (DIFF) scattergrams. In contrast, samples infected with Plasmodium falciparum (Pf) failed to show such DIFF scattergrams, or any other suggesting malaria infection (0/ 49 pts). Abnormal areas from DIFF scattergrams were related to the presence of mature schizonts and gametocytes, undestroyed by lysis agent, the latter not observed in Pf-infected patients from our series. The internal parameter WBC[DIFF] - WBC[BASO] raised in parallel to parasitemia in Pv, Po, and Pm samples but could not be used as a surrogate for parasitemia. In Pf infection, reticulocyte/ immature reticulocyte fraction (IRF) ratio showed a significant correlation with parasitemia (P < 0.05). A diagnostic model developed for Pf in endemic countries showed sensitivity of 77%. Using SYSMEX analyzers, Pv, Po, and Pm infections are easy to ascertain as DIFF scattergrams are almost specific (specificity = 99.9%). Pf infection diagnosis by CBC may be a more promising tool. © 2013 John Wiley & Sons Ltd.

  17. Establishment of Age- and Gender-Specific Reference Ranges for 36 Routine and 57 Cell Population Data Items in a New Automated Blood Cell Analyzer, Sysmex XN-2000.

    PubMed

    Park, Sang Hyuk; Park, Chan Jeoung; Lee, Bo Ra; Kim, Mi Jeong; Han, Min Young; Cho, Young Uk; Jang, Seongsoo

    2016-05-01

    We established age- and gender-specific reference ranges for the 36 routine complete blood cell (CBC) and 57 cell population data (CPD) items in the Sysmex XN-2000 (Sysmex, Japan). In total, 280 peripheral blood samples were obtained from an equal number of healthy adults. Values for 36 routine items and 57 CPD items were obtained for each sample, and the results were categorized into six subgroups (N>39 in each subgroup) according to patient age (20-40, 41-60, and >60 yr) and gender (male and female), and compared with respect to age and gender differences. The majority of data items (22 of 36 routine CBC items and 44 of 57 CPD items) exhibited significant differences (P≤0.05) in their results with respect to age or gender, and several red cell-, lymphocyte-, and platelet-related data tended to decrease in women or older adults. These results provide a basis for establishing age- and gender-specific reference ranges for routine and CPD items in Sysmex XN-2000. Furthermore, these reference ranges could be used to determine clinical significance for new items of Sysmex XN-2000 in further studies.

  18. Establishment of Age- and Gender-Specific Reference Ranges for 36 Routine and 57 Cell Population Data Items in a New Automated Blood Cell Analyzer, Sysmex XN-2000

    PubMed Central

    Park, Sang Hyuk; Lee, Bo-Ra; Kim, Mi-Jeong; Han, Min-Young; Cho, Young-Uk; Jang, Seongsoo

    2016-01-01

    We established age- and gender-specific reference ranges for the 36 routine complete blood cell (CBC) and 57 cell population data (CPD) items in the Sysmex XN-2000 (Sysmex, Japan). In total, 280 peripheral blood samples were obtained from an equal number of healthy adults. Values for 36 routine items and 57 CPD items were obtained for each sample, and the results were categorized into six subgroups (N>39 in each subgroup) according to patient age (20-40, 41-60, and >60 yr) and gender (male and female), and compared with respect to age and gender differences. The majority of data items (22 of 36 routine CBC items and 44 of 57 CPD items) exhibited significant differences (P≤0.05) in their results with respect to age or gender, and several red cell-, lymphocyte-, and platelet-related data tended to decrease in women or older adults. These results provide a basis for establishing age- and gender-specific reference ranges for routine and CPD items in Sysmex XN-2000. Furthermore, these reference ranges could be used to determine clinical significance for new items of Sysmex XN-2000 in further studies. PMID:26915613

  19. 14 CFR 121.661 - Initial approach altitude: Flag operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Initial approach altitude: Flag operations... § 121.661 Initial approach altitude: Flag operations. When making an initial approach to a radio navigation facility under IFR, no person may descend below the pertinent minimum altitude for...

  20. 14 CFR 121.661 - Initial approach altitude: Flag operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Initial approach altitude: Flag operations... § 121.661 Initial approach altitude: Flag operations. When making an initial approach to a radio navigation facility under IFR, no person may descend below the pertinent minimum altitude for...

  1. 14 CFR 121.661 - Initial approach altitude: Flag operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Initial approach altitude: Flag operations... § 121.661 Initial approach altitude: Flag operations. When making an initial approach to a radio navigation facility under IFR, no person may descend below the pertinent minimum altitude for...

  2. 14 CFR 121.661 - Initial approach altitude: Flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Initial approach altitude: Flag operations... § 121.661 Initial approach altitude: Flag operations. When making an initial approach to a radio navigation facility under IFR, no person may descend below the pertinent minimum altitude for...

  3. 14 CFR 121.661 - Initial approach altitude: Flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Initial approach altitude: Flag operations... § 121.661 Initial approach altitude: Flag operations. When making an initial approach to a radio navigation facility under IFR, no person may descend below the pertinent minimum altitude for...

  4. A Comparison of Injuries between Flag and Touch Football.

    ERIC Educational Resources Information Center

    Martin, Stephen L.

    This study was designed to determine whether fewer and less serious injuries result from participation in touch football as compared with flag football. A survey was taken of 30 flag football games and 30 touch football games and the incidence of injuries was recorded on a checklist. Results of the survey suggest the following: (a) intramural or…

  5. FIRE! A Red Flag Tap in Reclaiming Intervention

    ERIC Educational Resources Information Center

    Bodnar, Brian

    2007-01-01

    "Red Flag Interventions" address problems which are imported from elsewhere and acted out towards persons who are in effect innocent bystanders. This is commonly seen as students "carry in" problems from the home or street to school, or they "carry over" conflicts from one class to the next. A third variation of Red Flag intervention is when a…

  6. 10 CFR 1.55 - Establishment of official NRC flag.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Establishment of official NRC flag. 1.55 Section 1.55 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION NRC Seal and Flag... dark blue field with a gold fringe....

  7. FIRE! A Red Flag Tap in Reclaiming Intervention

    ERIC Educational Resources Information Center

    Bodnar, Brian

    2007-01-01

    "Red Flag Interventions" address problems which are imported from elsewhere and acted out towards persons who are in effect innocent bystanders. This is commonly seen as students "carry in" problems from the home or street to school, or they "carry over" conflicts from one class to the next. A third variation of Red Flag intervention is when a…

  8. Continued Legal Developments on the School Flag Movement.

    ERIC Educational Resources Information Center

    Knowles, Trudy

    1992-01-01

    Traces the legal history of the school flag movement and efforts of religious groups to have the mandatory salute abolished. Suggests that schools must educate students for true citizenship and what it means to live in a democracy. Argues that the flag should be a symbol of freedom rather than the object of empty pledges. (DK)

  9. Effects of morphology on the flapping dynamics of inverted flags

    NASA Astrophysics Data System (ADS)

    Fan, Boyu; Cosse, Julia; Sader, John; Gharib, Morteza

    2014-11-01

    The behavior of inverted flags has received recent attention in the study of the interaction of flexible bodies with fluid flows. It has implications in a variety of natural phenomena, such as the fluttering of leaves in the wind. As opposed to a conventional flag, defined by a fixed leading edge and a free trailing edge, an inverted flag has a free leading edge and a fixed trailing edge. The reversed flow orientation of inverted flags has led to a surprising observation. Over a narrow range of wind speeds, they exhibit a large-amplitude flapping motion that is not present in their conventional counterparts. Our study experimentally investigates the effects of flag morphology on the flapping behavior of inverted flags. Different flags ranging from rectangles to triangles are studied in a wind tunnel to assess the underlying parameters that govern their dynamics. We observe a significant shift in the limit-cycle flapping mode that is a function of flag shape parameters. This research is supported by the Lester Lees Aeronautics Summer Undergraduate Research Fellowship and the Gordon and Betty Moore Foundation.

  10. 10 CFR 1.55 - Establishment of official NRC flag.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Establishment of official NRC flag. 1.55 Section 1.55 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION NRC Seal and Flag... dark blue field with a gold fringe. ...

  11. 10 CFR 1.55 - Establishment of official NRC flag.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Establishment of official NRC flag. 1.55 Section 1.55 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION NRC Seal and Flag... dark blue field with a gold fringe. ...

  12. 10 CFR 1.55 - Establishment of official NRC flag.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Establishment of official NRC flag. 1.55 Section 1.55 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION NRC Seal and Flag... dark blue field with a gold fringe. ...

  13. 10 CFR 1.55 - Establishment of official NRC flag.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Establishment of official NRC flag. 1.55 Section 1.55 Energy NUCLEAR REGULATORY COMMISSION STATEMENT OF ORGANIZATION AND GENERAL INFORMATION NRC Seal and Flag... dark blue field with a gold fringe. ...

  14. 36 CFR 504.9 - Placards, signs, banners and flags.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Placards, signs, banners and flags. 504.9 Section 504.9 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING SMITHSONIAN INSTITUTION BUILDINGS AND GROUNDS § 504.9 Placards, signs, banners and flags...

  15. 36 CFR 504.9 - Placards, signs, banners and flags.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Placards, signs, banners and flags. 504.9 Section 504.9 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING SMITHSONIAN INSTITUTION BUILDINGS AND GROUNDS § 504.9 Placards, signs, banners and flags...

  16. 36 CFR 504.9 - Placards, signs, banners and flags.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Placards, signs, banners and flags. 504.9 Section 504.9 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING SMITHSONIAN INSTITUTION BUILDINGS AND GROUNDS § 504.9 Placards, signs, banners and flags...

  17. 36 CFR 504.9 - Placards, signs, banners and flags.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Placards, signs, banners and flags. 504.9 Section 504.9 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING SMITHSONIAN INSTITUTION BUILDINGS AND GROUNDS § 504.9 Placards, signs, banners and flags...

  18. Static shapes and instability of hanging flags conveying fluid

    NASA Astrophysics Data System (ADS)

    Yu, Tony S.; Jung, Sunghwan; Clanet, Christophe

    2008-11-01

    We investigate the interaction between a fluid jet and a long, thin sheet, i.e. ``flag'', clamped to the jet-nozzle. The jet wets and flows along one side of the flag from base (clamped end) to tip, which hangs freely. Experiments reveal a lateral force at the ``free'' end of the hanging flag; this force arises from the minimization of surface-energy as the jet detaches from the flag surface. At low Weber numbers (i.e. low flow rates), the tip force produces stable, static flag shapes with wavelengths inversely proportional to the flow rate. The static shapes are well described by a simple model coupling linear beam bending and inviscid flow. At higher Weber numbers, static shapes become unstable, leading to periodic oscillations analogous to previous work on hanging cantilever tubes conveying fluid (Paidoussis, 1970; Doaré and Langre, 2002). The observed critical flow rates for instability agree well with those predicted by linear stability analysis.

  19. 78 FR 17931 - Information Collection; Open Government Citizen Engagement Ratings, Rankings, and Flagging

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-25

    ... ADMINISTRATION Information Collection; Open Government Citizen Engagement Ratings, Rankings, and Flagging AGENCY... regarding open government citizen engagement ratings, rankings, and flagging. DATES: Comments must be...- 0288, Open Government Citizen Engagement Ratings, Rankings, and Flagging, by any of the...

  20. Optimization of laboratory workflow in clinical hematology laboratory with reduced manual slide review: comparison between Sysmex XE-2100 and ABX Pentra DX120.

    PubMed

    Hur, M; Cho, J-H; Kim, H; Hong, M-H; Moon, H-W; Yun, Y-M; Kim, J Q

    2011-08-01

    The validation of automated hematology analyzer results by manual slide review (MSR) is currently an inevitable work process in clinical hematology laboratories. The laboratory workload would be optimized if the requirement for MSR could be reduced without compromising patient care. We investigated whether slide-making rates would be different between two hematology analyzers, which were paired with their own automated slide makers/stainers: Sysmex XE-2100 with SP-1000i (Sysmex, Kobe, Japan) and ABX Pentra DX120 with SPS evolution (ABX-Horiba, Montpellier, France). A total of 943 samples were run in parallel on the Sysmex XE-2100 and ABX Pentra DX120. Reflex slides were automatically made in each analyzer according to its own criteria, which reflected the criteria of MSR in our laboratory. The slide-making rates were compared, and the results were further confirmed using the criteria of MSR. The slide-making rates in Sysmex XE-2100, ABX Pentra DX120, and manual review were 22.5% (212/943), 15.91% (150/943), and 11.5% (108/943), respectively. In 774 (82.1%) samples, the three methods showed concordant results, and all made slides in 82 samples. Using the manual method as a standard, the sensitivity and specificity were 86.1% and 85.8% in Sysmex XE-2100 and 89.8% and 93.7% in ABX Pentra DX120. Our data show that the slide-making rates are variable in different hematology analyzers. It also implies that although MSR cannot be fully substituted by modern hematology analyzers, it can be effectively reduced to optimize laboratory workload. © 2011 Blackwell Publishing Ltd.

  1. Phosphorus remobilization from rice flag leaves during grain filling: an RNA-seq study.

    PubMed

    Jeong, Kwanho; Baten, Abdul; Waters, Daniel L E; Pantoja, Omar; Julia, Cecile C; Wissuwa, Matthias; Heuer, Sigrid; Kretzschmar, Tobias; Rose, Terry J

    2017-01-01

    The physiology and molecular regulation of phosphorus (P) remobilization from vegetative tissues to grains during grain filling is poorly understood, despite the pivotal role it plays in the global P cycle. To test the hypothesis that a subset of genes involved in the P starvation response are involved in remobilization of P from flag leaves to developing grains, we conducted an RNA-seq analysis of rice flag leaves during the preremobilization phase (6 DAA) and when the leaves were acting as a P source (15 DAA). Several genes that respond to phosphate starvation, including three purple acid phosphatases (OsPAP3, OsPAP9b and OsPAP10a), were significantly up-regulated at 15 DAA, consistent with a role in remobilization of P from flag leaves during grain filling. A number of genes that have not been implicated in the phosphate starvation response, OsPAP26, SPX-MFS1 (a putative P transporter) and SPX-MFS2, also showed expression profiles consistent with involvement in P remobilization from senescing flag leaves. Metabolic pathway analysis using the KEGG system suggested plastid membrane lipid synthesis is a critical process during the P remobilization phase. In particular, the up-regulation of OsPLDz2 and OsSQD2 at 15 DAA suggested phospholipids were being degraded and replaced by other lipids to enable continued cellular function while liberating P for export to developing grains. Three genes associated with RNA degradation that have not previously been implicated in the P starvation response also showed expression profiles consistent with a role in P mobilization from senescing flag leaves. © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  2. Structure of anti-FLAG M2 Fab domain and its use in the stabilization of engineered membrane proteins

    SciTech Connect

    Roosild, Tarmo P.; Castronovo, Samantha; Choe, Senyon

    2006-09-01

    The X-ray crystallographic analysis of anti-FLAG M2 Fab is reported and the implications of the structure on FLAG epitope binding are described as a first step in the development of a tool for the structural and biophysical study of membrane proteins. The inherent difficulties of stabilizing detergent-solubilized integral membrane proteins for biophysical or structural analysis demand the development of new methodologies to improve success rates. One proven strategy is the use of antibody fragments to increase the ‘soluble’ portion of any membrane protein, but this approach is limited by the difficulties and expense associated with producing monoclonal antibodies to an appropriate exposed epitope on the target protein. Here, the stabilization of a detergent-solubilized K{sup +} channel protein, KvPae, by engineering a FLAG-binding epitope into a known loop region of the protein and creating a complex with Fab fragments from commercially available anti-FLAG M2 monoclonal antibodies is reported. Although well diffracting crystals of the complex have not yet been obtained, during the course of crystallization trials the structure of the anti-FLAG M2 Fab domain was solved to 1.86 Å resolution. This structure, which should aid future structure-determination efforts using this approach by facilitating molecular-replacement phasing, reveals that the binding pocket appears to be specific only for the first four amino acids of the traditional FLAG epitope, namely DYKD. Thus, the use of antibody fragments for improving the stability of target proteins can be rapidly applied to the study of membrane-protein structure by placing the short DKYD motif within a predicted peripheral loop of that protein and utilizing commercially available anti-FLAG M2 antibody fragments.

  3. Transcriptome Phase Distribution Analysis Reveals Diurnal Regulated Biological Processes and Key Pathways in Rice Flag Leaves and Seedling Leaves

    PubMed Central

    Li, Meina; Xing, Zhuo; Yang, Wenqiang; Chen, Guang; Guo, Han; Gong, Xiaojie; Du, Zhou; Zhang, Zhenhai; Hu, Xingming; Wang, Dong; Qian, Qian; Wang, Tai; Su, Zhen; Xue, Yongbiao

    2011-01-01

    Plant diurnal oscillation is a 24-hour period based variation. The correlation between diurnal genes and biological pathways was widely revealed by microarray analysis in different species. Rice (Oryza sativa) is the major food staple for about half of the world's population. The rice flag leaf is essential in providing photosynthates to the grain filling. However, there is still no comprehensive view about the diurnal transcriptome for rice leaves. In this study, we applied rice microarray to monitor the rhythmically expressed genes in rice seedling and flag leaves. We developed a new computational analysis approach and identified 6,266 (10.96%) diurnal probe sets in seedling leaves, 13,773 (24.08%) diurnal probe sets in flag leaves. About 65% of overall transcription factors were identified as flag leaf preferred. In seedling leaves, the peak of phase distribution was from 2:00am to 4:00am, whereas in flag leaves, the peak was from 8:00pm to 2:00am. The diurnal phase distribution analysis of gene ontology (GO) and cis-element enrichment indicated that, some important processes were waken by the light, such as photosynthesis and abiotic stimulus, while some genes related to the nuclear and ribosome involved processes were active mostly during the switch time of light to dark. The starch and sucrose metabolism pathway genes also showed diurnal phase. We conducted comparison analysis between Arabidopsis and rice leaf transcriptome throughout the diurnal cycle. In summary, our analysis approach is feasible for relatively unbiased identification of diurnal transcripts, efficiently detecting some special periodic patterns with non-sinusoidal periodic patterns. Compared to the rice flag leaves, the gene transcription levels of seedling leaves were relatively limited to the diurnal rhythm. Our comprehensive microarray analysis of seedling and flag leaves of rice provided an overview of the rice diurnal transcriptome and indicated some diurnal regulated biological

  4. Transcriptome phase distribution analysis reveals diurnal regulated biological processes and key pathways in rice flag leaves and seedling leaves.

    PubMed

    Xu, Wenying; Yang, Rendong; Li, Meina; Xing, Zhuo; Yang, Wenqiang; Chen, Guang; Guo, Han; Gong, Xiaojie; Du, Zhou; Zhang, Zhenhai; Hu, Xingming; Wang, Dong; Qian, Qian; Wang, Tai; Su, Zhen; Xue, Yongbiao

    2011-03-02

    Plant diurnal oscillation is a 24-hour period based variation. The correlation between diurnal genes and biological pathways was widely revealed by microarray analysis in different species. Rice (Oryza sativa) is the major food staple for about half of the world's population. The rice flag leaf is essential in providing photosynthates to the grain filling. However, there is still no comprehensive view about the diurnal transcriptome for rice leaves. In this study, we applied rice microarray to monitor the rhythmically expressed genes in rice seedling and flag leaves. We developed a new computational analysis approach and identified 6,266 (10.96%) diurnal probe sets in seedling leaves, 13,773 (24.08%) diurnal probe sets in flag leaves. About 65% of overall transcription factors were identified as flag leaf preferred. In seedling leaves, the peak of phase distribution was from 2:00am to 4:00am, whereas in flag leaves, the peak was from 8:00pm to 2:00am. The diurnal phase distribution analysis of gene ontology (GO) and cis-element enrichment indicated that, some important processes were waken by the light, such as photosynthesis and abiotic stimulus, while some genes related to the nuclear and ribosome involved processes were active mostly during the switch time of light to dark. The starch and sucrose metabolism pathway genes also showed diurnal phase. We conducted comparison analysis between Arabidopsis and rice leaf transcriptome throughout the diurnal cycle. In summary, our analysis approach is feasible for relatively unbiased identification of diurnal transcripts, efficiently detecting some special periodic patterns with non-sinusoidal periodic patterns. Compared to the rice flag leaves, the gene transcription levels of seedling leaves were relatively limited to the diurnal rhythm. Our comprehensive microarray analysis of seedling and flag leaves of rice provided an overview of the rice diurnal transcriptome and indicated some diurnal regulated biological

  5. Self-propulsion of a heaving and pitching flexible flag

    NASA Astrophysics Data System (ADS)

    Kim, Boyoung; Park, Sung Goon; Sung, Hyung Jin

    2015-11-01

    Flapping motions of flexible flags are widespread in nature. Birds, fish, and insects use their wings, fins, or bodies to stay afloat and to advance forward in the surrounding fluids. In the present study, a self-propelled flexible flag with heaving and pitching motions in a quiescent flow has been simulated by using the immersed boundary method. The flexible flag can move freely in the horizontal direction and the body of the flexible flag moves passively along the head. The motion of the head of the flag was described as a harmonic heaving oscillation in the vertical direction. The motion of the angle of the head was described as a harmonic oscillation with a moving clamped condition for the heaving and pitching flag. The cruising speed and the swimming efficiency of the self-propelled flag were determined as functions of the bending coefficient (γ) , the heaving amplitude (Ah) , the pitching amplitude (Ap) , the heaving frequency (St), and the phase difference (Δϕ) between Ah and Ap. We conducted a parametric study on the optimized the cruising speed and the swimming efficiency with respect to γ, St, Ah, Ap, and Δϕ

  6. Flapping dynamics of an inverted flag in a uniform flow

    NASA Astrophysics Data System (ADS)

    Ryu, Jaeha; Park, Sung Goon; Kim, Boyoung; Sung, Hyung Jin

    2015-11-01

    Much research in recent years has focused on the flow dynamics of flexible structures in a uniform flow and particularly on the flow dynamics related to energy harvesting systems. An energy harvesting system comprising piezoelectric patches attached to the surface of a flexible structure can convert the energy stored in solid deformations into an electric current that powers a purely resistive output circuit. Recently, an inverted flag which has the freely moving leading edge and the clamped trailing edge was suggested. The inverted flag improved the amount of strain energy that was converted into the flag deformations from the surrounding fluid. In this study, the flapping dynamics of an inverted flag in a uniform flow were simulated using the immersed boundary method. The flapping dynamics of and vortical structures around the inverted flag were examined in terms of the bending rigidity and the Reynolds number. The strain energy of the inverted flag and the proportion of the strain energy of the inverted flag to the kinetic energy of the flow were considered as an indicator of the energy harvesting system efficiency.

  7. [Influence of reagent storage in Sysmex CA7000 for different time on 4 test RESULTS: of the plasma coagulation].

    PubMed

    Chen, Tong-Qing; Li, Zhen-Xing

    2014-12-01

    The purpose of this study was to investigate the influence of blood coagulation reagents stored for different time on test results of the specimens prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (Fib). A total of 21 patient plasma specimens was taken and measured for homeostasis by Sysmex CA7000 automated blood coagulation analyzer and supporting reagent. The PT, APTT, TT and Fib of specimens were measured with the reagents stored in Sysmex CA7000 for different time. The differences of PT, APTT, TT and Fib were analyzed between values measured of the reagents stored for 0 hour and different time (TS:12, 24, 36,48, 60, 72 h; DA:24, 48, 72, 96, 120 h; TT:2, 4, 6, 8, 10, 12 h; TR:4, 8, 12, 16, 20, 24 h; OVB:1, 2, 3, 4, 5 ,6 h), respectively. The results showed that when coagulation reagent TS were stored for more than 48 h , DA 96 h, TT 10 h, TR 16 h and OVB 4 h, the values of PT, APTT, TT and Fib of samples were statistically different from the values measured with fresh coagulation reagent (P < 0.01), respectively. Compared 0 h with TS stored for 48-72, DA 96-120, TT 10-12, TR 16-24 and OVB 4-6 h, the percentage difference of PT, APTT, TT and Fib is in -2.6% ∼ 10.8%, -3.44% ∼ 4.8%, -3.9% ∼ 5.52%, -10.8% ∼ 3.3% and -17.2% ∼ 0.5%, the PT and Fib changes were more significant. Accordingly, the result of PT, APTT and TT had a uptrend as the reagent stored in Sysmex CA7000 analyzer for a long time, while Fib downtrend. It is concluded that the reagents showed be timely replaced when the plasma coagulation test is performed so as to obtain accurate results of examination.

  8. Diagnosing feline infectious peritonitis using the Sysmex XT-2000iV based on frozen supernatants from cavitary effusions.

    PubMed

    Stranieri, Angelica; Paltrinieri, Saverio; Giordano, Alessia

    2017-02-01

    The delta total nucleated cells (ΔTNC) measurement with the Sysmex XT-2000iV (Sysmex Europe, Norderstedt, Germany) has high diagnostic accuracy on effusions in feline infectious peritonitis (FIP) cases, but the test can be performed only on fresh samples. We evaluated whether supernatants from effusions retain the ability to induce cell clumping and assessed the diagnostic accuracy of this modified ΔTNC method. Effusions were collected from FIP cats ( n = 19) and from cats with other diseases ( n = 15). ΔTNC was measured on fresh samples and on frozen-thawed supernatants after the addition of feline blood at 1:10 dilution. Diagnostic accuracy was assessed at the cutoffs of suggestive of FIP (ΔTNC = 1.7) and consistent with FIP (ΔTNC = 3.4). The influence of the protein content, number of added cells, and magnitude of dilution were also investigated. Specificity and positive predictive value were 100% for both the methods. Sensitivity and negative predictive value were higher for the modified ΔTNC (84.2% and 83.3%, respectively, at the cutoff of 1.7; 78.9% and 78.9%, respectively, at the cutoff of 3.4) than for the ΔTNC on fresh samples (78.6% and 81.3%, respectively, at the cutoff of 1.7; 57.1% and 68.4%, respectively, at the cutoff of 3.4). Protein content, total cell count of the added blood, and magnitude of dilutions did not influence the results. Supernatants of frozen effusions from FIP cats retain the ability to induce cell clumping, thus the modified ΔTNC measurement is a reliable tool to diagnose FIP on samples that cannot be analyzed immediately.

  9. Measurement of immature platelets with Abbott CD-Sapphire and Sysmex XE-5000 in haematology and oncology patients.

    PubMed

    Meintker, Lisa; Haimerl, Maria; Ringwald, Jürgen; Krause, Stefan W

    2013-11-01

    Measurement of immature platelets was introduced into routine diagnostics by Sysmex as immature platelet fraction (IPF) some years ago and recently by Abbott as reticulated platelet fraction (rPT). Here, we compare both methods. We evaluated the precision and agreement of these parameters between Sysmex XE-5000 and Abbott CD-Sapphire in three distinct thrombocytopaenic cohorts: 30 patients with beginning thrombocytopaenia and 64 patients with recovering platelets (PLT) after chemotherapy, 16 patients with immune thrombocytopaenia (ITP) or heparin-induced thrombocytopaenia type 2 (HIT) and 110 additional normal controls. Furthermore, we analysed, how IPF/rPT differed between these thrombocytopaenic cohorts and controls. Both analysers demonstrated acceptable overall precision (repeatability) of IPF/rPT with lower precision at low PLT counts. IPF/rPT artificially increased during storage of blood samples overnight. Inter-instrument comparison showed a moderate correlation (Pearson r²=0.38) and a systematic bias of 1.04 towards higher IPF-values with the XE-5000. IPF/rPT was highest in recovering thrombopoesis after chemotherapy and moderately increased in ITP/HIT. The normal range deduced from control samples was much narrower with CD-Sapphire (1.0%-3.8%, established here for the first time) in comparison to XE-5000 (0.8%-7.9%) leading to a smaller overlap of samples with increased PLT turnover and normal controls. IPF and rPT both give useful information on PLT turnover, although the two analysers only show a moderate inter-instrument correlation and have different reference ranges. A better separation of patient groups with high PLT turnover like ITP/HIT from normal controls is obtained by CD-Sapphire.

  10. SOUTH ELEVATION. THE DWELLING, FLAG TOWER, AND HAZARDOUS MATERIAL STORAGE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    SOUTH ELEVATION. THE DWELLING, FLAG TOWER, AND HAZARDOUS MATERIAL STORAGE SHED ARE VISIBLE IN THE BACKGROUND. - U.S. Coast Guard Lake Worth Inlet Station, Boathouse, Peanut Island, Riviera Beach, Palm Beach County, FL

  11. WEST ELEVATION. DWELLING AND FLAG TOWER ARE IN THE DISTANCE. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    WEST ELEVATION. DWELLING AND FLAG TOWER ARE IN THE DISTANCE. HAZARDOUS MATERIAL STORAGE SHED IS IN THE FOREGROUND. - U.S. Coast Guard Lake Worth Inlet Station, Boathouse, Peanut Island, Riviera Beach, Palm Beach County, FL

  12. Interior detail of platform in main hall, with desk, flag, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior detail of platform in main hall, with desk, flag, and banners, facing south - International Longshoremen's & Warehousemen's Union Hall, Naval Civil Engineering Laboratory, Port Hueneme Road, Port Hueneme, Ventura County, CA

  13. View of the north tower porte cochere and flag tower, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of the north tower porte cochere and flag tower, looking southwest (duplicate of HABS No. DC-141-19) - Smithsonian Institution Building, 1000 Jefferson Drive, between Ninth & Twelfth Streets, Southwest, Washington, District of Columbia, DC

  14. 14 CFR 121.685 - Aircraft record: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Aircraft record: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.685 Aircraft record: Domestic and flag operations. Each certificate holder conducting domestic or flag...

  15. 14 CFR 121.685 - Aircraft record: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Aircraft record: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.685 Aircraft record: Domestic and flag operations. Each certificate holder conducting domestic or flag...

  16. 14 CFR 121.99 - Communications facilities-domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Communications facilities-domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Approval of Routes: Domestic and Flag Operations § 121.99 Communications facilities—domestic and flag operations. (a) Each certificate...

  17. 14 CFR 121.99 - Communications facilities-domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Communications facilities-domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Approval of Routes: Domestic and Flag Operations § 121.99 Communications facilities—domestic and flag operations. (a) Each certificate...

  18. 46 CFR 282.10 - Basis for determining foreign-flag competition.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Basis for determining foreign-flag competition. 282.10... SERVICES IN THE FOREIGN COMMERCE OF THE UNITED STATES Foreign-Flag Competition § 282.10 Basis for determining foreign-flag competition. The foreign-flag competition shall form the basis for determining the...

  19. 46 CFR 153.16 - Requirements for foreign flag vessel permits.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Requirements for foreign flag vessel permits. 153.16... Requirements for foreign flag vessel permits. To have its Certificate of Compliance endorsed to carry a cargo listed in Table 1, a foreign flag vessel must: (a) Have an IMO Certificate, if the flag administration...

  20. 46 CFR 154.1802 - Certificates, letters and endorsements: Foreign flag vessels.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Certificates, letters and endorsements: Foreign flag... Operations § 154.1802 Certificates, letters and endorsements: Foreign flag vessels. (a) No person may operate on the navigable waters of the United States a foreign flag vessel, whose flag administration issues...

  1. 41 CFR 301-10.134 - What is U.S. flag air carrier service?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 4 2010-07-01 2010-07-01 false What is U.S. flag air... Common Carrier Transportation Use of United States Flag Air Carriers § 301-10.134 What is U.S. flag air carrier service? U.S. flag air carrier service is service provided on an air carrier which holds a...

  2. 46 CFR 282.10 - Basis for determining foreign-flag competition.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 8 2011-10-01 2011-10-01 false Basis for determining foreign-flag competition. 282.10... SERVICES IN THE FOREIGN COMMERCE OF THE UNITED STATES Foreign-Flag Competition § 282.10 Basis for determining foreign-flag competition. The foreign-flag competition shall form the basis for determining...

  3. Efficiency of flagging and dragging for tick collection.

    PubMed

    Dantas-Torres, Filipe; Lia, Riccardo Paolo; Capelli, Gioia; Otranto, Domenico

    2013-09-01

    It is acknowledged that data from field studies on tick ecology might be biased by collection methods, but actually comparative studies are still limited. Herein we assessed whether the efficiency of flagging and dragging varies according to tick developmental stage, species, season and habitat. Ticks were collected in three sites bordered by an oak forest. The abundance of ticks collected by each collection method varied according to tick species, developmental stage, season, and habitat. Flagging was in general more efficient in collecting adult ticks, especially in spring and winter. Females were more frequently collected by flagging in the meadow and grassland habitats and males in the man-made trail. Flagging collected significantly more adults of Dermacentor marginatus, Hyalomma marginatum, Haemaphysalis inermis and Ixodes ricinus. Flagging was more efficient in collecting D. marginatus and I. ricinus in spring, and H. inermis and I. ricinus females in both spring and winter. In summer and autumn tick abundances were generally similar, with the exception of D. marginatus female in autumn. Flagging was more efficient in collecting D. marginatus adults in the meadow habitat and in the man-made trail, and I. ricinus adults in the meadow and grassland habitats. Dragging was more efficient in grassland for R. turanicus. Our results suggest that variations in terms of collection method performance are associated to factors linked to tick behaviour, habitat characteristics, and climate. Field studies employing these collection methods should take this into account to avoid misleading conclusions about tick population dynamics and tick-borne pathogen transmission risk.

  4. High explosive programmed burn in the FLAG code

    SciTech Connect

    Mandell, D.; Burton, D.; Lund, C.

    1998-02-01

    The models used to calculate the programmed burn high-explosive lighting times for two- and three-dimensions in the FLAG code are described. FLAG uses an unstructured polyhedra grid. The calculations were compared to exact solutions for a square in two dimensions and for a cube in three dimensions. The maximum error was 3.95 percent in two dimensions and 4.84 percent in three dimensions. The high explosive lighting time model described has the advantage that only one cell at a time needs to be considered.

  5. Development of a validation algorithm for 'present on admission' flagging

    PubMed Central

    2009-01-01

    Background The use of routine hospital data for understanding patterns of adverse outcomes has been limited in the past by the fact that pre-existing and post-admission conditions have been indistinguishable. The use of a 'Present on Admission' (or POA) indicator to distinguish pre-existing or co-morbid conditions from those arising during the episode of care has been advocated in the US for many years as a tool to support quality assurance activities and improve the accuracy of risk adjustment methodologies. The USA, Australia and Canada now all assign a flag to indicate the timing of onset of diagnoses. For quality improvement purposes, it is the 'not-POA' diagnoses (that is, those acquired in hospital) that are of interest. Methods Our objective was to develop an algorithm for assessing the validity of assignment of 'not-POA' flags. We undertook expert review of the International Classification of Diseases, 10th Revision, Australian Modification (ICD-10-AM) to identify conditions that could not be plausibly hospital-acquired. The resulting computer algorithm was tested against all diagnoses flagged as complications in the Victorian (Australia) Admitted Episodes Dataset, 2005/06. Measures reported include rates of appropriate assignment of the new Australian 'Condition Onset' flag by ICD chapter, and patterns of invalid flagging. Results Of 18,418 diagnosis codes reviewed, 93.4% (n = 17,195) reflected agreement on status for flagging by at least 2 of 3 reviewers (including 64.4% unanimous agreement; Fleiss' Kappa: 0.61). In tests of the new algorithm, 96.14% of all hospital-acquired diagnosis codes flagged were found to be valid in the Victorian records analysed. A lower proportion of individual codes was judged to be acceptably flagged (76.2%), but this reflected a high proportion of codes used <5 times in the data set (789/1035 invalid codes). Conclusion An indicator variable about the timing of occurrence of diagnoses can greatly expand the use of routinely

  6. A Comparison of Mindray BC-6800, Sysmex XN-2000, and Beckman Coulter LH750 Automated Hematology Analyzers: A Pediatric Study.

    PubMed

    Ciepiela, Olga; Kotuła, Iwona; Kierat, Szymon; Sieczkowska, Sandra; Podsiadłowska, Anna; Jenczelewska, Anna; Księżarczyk, Karolina; Demkow, Urszula

    2016-11-01

    Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray BC-6800, Sysmex XN-2000 and Beckman Coulter LH750 was performed. A comparison of results obtained by automated analysis of 807 anticoagulated blood samples from children and 125 manual microscopic differentiations were performed. This comparative study included white blood cell count, red blood cell count, and erythrocyte indices, as well as platelet count. The present study showed a poor level of agreement between white blood cell enumeration and differentiation of the three automated hematology analyzers under comparison. A very good agreement was found when comparing manual blood smear and automated granulocytes, monocytes, and lymphocytes differentiation. Red blood cell evaluation showed better agreement than white blood cells between the studied analyzers. To conclude, studied instruments did not ensure satisfactory interchangeability and did not facilitate a substitution of one analyzer by another. © 2016 Wiley Periodicals, Inc.

  7. Clinical significance of cell population data (CPD) on Sysmex XN-9000 in septic patients with our without liver impairment

    PubMed Central

    Seghezzi, Michela; Vavassori, Mauro; Dominoni, Paola; Apassiti Esposito, Sara; Manenti, Barbara; Mecca, Tommaso; Marchesi, Gianmariano; Castellucci, Enrico; Azzarà, Giovanna; Ottomano, Cosimo; Lippi, Giuseppe

    2016-01-01

    Background This study evaluated the clinical significance of cell population data (CPD) parameters obtained on Sysmex XN-9000 in septic patients admitted to intensive care unit (ICU) and stratified according to liver function. Methods The study population consisted in 84 patients, 44 of whom did not develop sepsis (NS), whereas the remaining 40 developed sepsis (SE) (n=24) or septic shock (SS) (n=16). Two hundred ostensibly healthy blood donors [healthy subjects (HS)], undergoing routine blood testing before a regular blood donation, were studied. Results Except for neutrophils and lymphocytes cell size (NE-FCS and LY-Z), all other CPD values were significantly different in ICU patients compared to HS. Neutrophils and monocytes fluorescence intensity (NE-SFL and MO-X) values were significantly higher in SS compared to sepsis and not develop sepsis patients. The value of many parameters was also different according to liver function. Overall, MO-X and neutrophils fluorescence intensity (NE-SFL) exhibited the best performance for diagnosing sepsis in all patients (AUC, 0.75 and 0.72), as well as in those with (AUC, 0.95 and 0.89) or without (AUC, 0.72 for both) liver impairment. These parameters were also significantly correlated with Sequential Organ Failure Assessment (SOFA) score. Conclusions This study suggested that some novel CPD parameters (namely NE-SFL and MO-X) may provide useful information for diagnosis and management of sepsis. PMID:27942509

  8. Use of hematopoietic progenitor cell count on the Sysmex XE-2100 for peripheral blood stem cell harvest monitoring.

    PubMed

    Letestu, Rémi; Marzac, Christophe; Audat, Françoise; Belhocine, Ramdane; Tondeur, Sylvie; Baccini, Véronique; Garçon, Loïc; Cortivo, Liliane Dal; Perrot, Jean-Yves; Lefrère, François; Valensi, Françoise; Ajchenbaum-Cymbalista, Florence

    2007-01-01

    Successful peripheral blood stem cell (PBSC) collection depends on the timing of apheresis based on CD34+ cell enumeration. Because this analysis is expensive and induces organization difficulties, we evaluated hematopoietic progenitor cell (HPC) quantification on the Sysmex XE-2100 as a surrogate analysis. We tested 157 blood samples for CD34+ cells and HPC counts. We found a good linear correlation between HPC and CD34+ and determined simple rules allowing to use HPC count in daily practice. We set a positive cut-off >30 HPC/mm(3) for allowing PBSC harvest and a negative cut-off at 0 HPC/mm(3) for which collection should be delayed. These two situations accounted for 62% of cases and CD34+ cell count by flow cytometry confirmed HPC result in 95% of cases. Between 0 and 30 HPC/mm3, CD34+ enumeration is required for decision-making. We conclude that HPC count may be a useful surrogate for CD34+ enumeration in PBSC harvest monitoring.

  9. Tips to Increase Girls' Participation in Flag Football Units

    ERIC Educational Resources Information Center

    Hannon, James C.; Ratliffe, Thomas

    2006-01-01

    Despite the apparent popularity of flag football as an activity in physical education class and football as an after-school offering for girls, studies related to gender stereotyping of sports have found overwhelming evidence indicating that football is perceived as a masculine activity among males and females in primary school, secondary school,…

  10. 46 CFR 153.9 - Foreign flag vessel endorsement application.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... endorsement from the cognizant Officer in Charge, Marine Inspection and have aboard the vessel copies of IMO Certificates issued by the vessel's administration and— (1) An additional classification society statement that... 46 Shipping 5 2014-10-01 2014-10-01 false Foreign flag vessel endorsement application....

  11. 46 CFR 153.9 - Foreign flag vessel endorsement application.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... endorsement from the cognizant Officer in Charge, Marine Inspection and have aboard the vessel copies of IMO Certificates issued by the vessel's administration and— (1) An additional classification society statement that... 46 Shipping 5 2012-10-01 2012-10-01 false Foreign flag vessel endorsement application....

  12. 46 CFR 153.9 - Foreign flag vessel endorsement application.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... endorsement from the cognizant Officer in Charge, Marine Inspection and have aboard the vessel copies of IMO Certificates issued by the vessel's administration and— (1) An additional classification society statement that... 46 Shipping 5 2013-10-01 2013-10-01 false Foreign flag vessel endorsement application....

  13. 46 CFR 153.9 - Foreign flag vessel endorsement application.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... endorsement from the cognizant Officer in Charge, Marine Inspection and have aboard the vessel copies of IMO Certificates issued by the vessel's administration and— (1) An additional classification society statement that... 46 Shipping 5 2011-10-01 2011-10-01 false Foreign flag vessel endorsement application....

  14. Red Flags in Prospective Contractor’s Financial Statement

    DTIC Science & Technology

    1989-12-01

    Increase in Intangible Asset Balances ........ ............... .. 26 2. Red Flags in Liability .... ........... .. 26 a. Current Liability...continued, can lead to operating difficulties. f. An Unusual Increase in Intangible Asset Balances An unusual increase in intangible asset balances...maintenance indicates profitability and potential operating difficulties. 15. An unusual increase in intangible asset balances signals that the company may be

  15. 14 CFR 1221.113 - Use of the NASA Flags.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Use of the NASA Flags. 1221.113 Section 1221.113 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA Program...

  16. 14 CFR 1221.106 - Establishment of the NASA Flag.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Establishment of the NASA Flag. 1221.106 Section 1221.106 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA...

  17. 14 CFR 1221.106 - Establishment of the NASA Flag.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Establishment of the NASA Flag. 1221.106 Section 1221.106 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA...

  18. 14 CFR 1221.106 - Establishment of the NASA Flag.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Establishment of the NASA Flag. 1221.106 Section 1221.106 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA...

  19. 14 CFR 1221.113 - Use of the NASA Flags.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Use of the NASA Flags. 1221.113 Section 1221.113 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA Program...

  20. 14 CFR 1221.113 - Use of the NASA Flags.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Use of the NASA Flags. 1221.113 Section 1221.113 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA Program...

  1. 14 CFR 1221.113 - Use of the NASA Flags.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Use of the NASA Flags. 1221.113 Section 1221.113 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA Program...

  2. 14 CFR 1221.106 - Establishment of the NASA Flag.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Establishment of the NASA Flag. 1221.106 Section 1221.106 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA...

  3. 46 CFR 153.9 - Foreign flag vessel endorsement application.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Certificates issued by the vessel's administration and— (1) An additional classification society statement that... Compliance endorsed with the name of an alkylene oxide; and (2) An additional classification society...) The name of the vessel's flag administration. (6) The name of the society that classes the vessel....

  4. Onboard photo: STS-50 crew portrait with American flag

    NASA Technical Reports Server (NTRS)

    1992-01-01

    Onboard Space Shuttle Columbia (STS-50) crewmembers rally around the American flag in the United States Microgravity Laboratory-1 (USML-1). Pictured are (from top, left to right) pilot Kerneth D. Bowersox; payload specialist Lawrence J. Delucas; commander Richard N. Richards; payload commander Bonnie J. Dunbar; mission specialists Carl J. Meade and Ellen S. Baker; and payload specialist Eugene H. Trinh.

  5. Modifying Flag Football for Gender Equitable Engagement in Secondary Schools

    ERIC Educational Resources Information Center

    Kahan, David

    2008-01-01

    Flag or touch football is a popular activity unit in American secondary physical education curricula. However, unlike other sports its stigmatization as a masculine-typed activity and frequent inequitable distribution of game play opportunities at the skill positions (e.g., receiver, quarterback) results in the marginalization of female…

  6. Tips to Increase Girls' Participation in Flag Football Units

    ERIC Educational Resources Information Center

    Hannon, James C.; Ratliffe, Thomas

    2006-01-01

    Despite the apparent popularity of flag football as an activity in physical education class and football as an after-school offering for girls, studies related to gender stereotyping of sports have found overwhelming evidence indicating that football is perceived as a masculine activity among males and females in primary school, secondary school,…

  7. Exposure to the American flag polarizes democratic-republican ideologies.

    PubMed

    Chan, Eugene Y

    2017-04-16

    Some prior research has suggested that exposure to the American flag tilts Americans towards Republicanism, while others have proffered that it brings outs a common 'together' perspective instead. We explore a third possibility - that it may actually polarize Americans' political ideology. It is generally accepted that exposure to an environmental cue can shift attitudes and behaviours, at least partly or temporarily, in a manner that is consistent with that cue. Yet, the same cue can mean different things to different people. In the same vein, given how national identity and political ideology are intertwined in the United States, we hypothesize that the American flag should heighten different political beliefs depending on individuals' political ideology. To Democrats, being American is to support Democratic values, but to Republicans, being American is to support Republican values. The American flag thus should heighten Democrats of their Democratic identity, and it should heighten Republicans of their Republican one. The results of an experiment with 752 American respondents who were representative of the US population supported this polarizing effect of the American flag. The theoretical and policy implications of the findings are offered. © 2017 The British Psychological Society.

  8. 10 CFR 1002.21 - Description of distinguishing flag.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... shall be of rayon banner, measure 4′4″ on hoist by 5′6″ on the fly, exclusive of heading and hems, and be fringed on three edges with yellow rayon fringe, 21/2″ wide. (2) The outdoor flag shall be...

  9. Modifying Flag Football for Gender Equitable Engagement in Secondary Schools

    ERIC Educational Resources Information Center

    Kahan, David

    2008-01-01

    Flag or touch football is a popular activity unit in American secondary physical education curricula. However, unlike other sports its stigmatization as a masculine-typed activity and frequent inequitable distribution of game play opportunities at the skill positions (e.g., receiver, quarterback) results in the marginalization of female…

  10. 36 CFR 520.10 - Placards, signs, banners, and flags.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Placards, signs, banners, and flags. 520.10 Section 520.10 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING THE BUILDINGS AND GROUNDS OF THE NATIONAL ZOOLOGICAL PARK OF THE SMITHSONIAN INSTITUTION...

  11. 36 CFR 520.10 - Placards, signs, banners, and flags.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Placards, signs, banners, and flags. 520.10 Section 520.10 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING THE BUILDINGS AND GROUNDS OF THE NATIONAL ZOOLOGICAL PARK OF THE SMITHSONIAN INSTITUTION...

  12. 36 CFR 520.10 - Placards, signs, banners, and flags.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Placards, signs, banners, and flags. 520.10 Section 520.10 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING THE BUILDINGS AND GROUNDS OF THE NATIONAL ZOOLOGICAL PARK OF THE SMITHSONIAN INSTITUTION...

  13. 36 CFR 520.10 - Placards, signs, banners, and flags.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Placards, signs, banners, and flags. 520.10 Section 520.10 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING THE BUILDINGS AND GROUNDS OF THE NATIONAL ZOOLOGICAL PARK OF THE SMITHSONIAN INSTITUTION...

  14. 46 CFR 381.5 - Fix American-flag tonnage first.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... preference cargoes required by that Act and the Food Security Act of 1985 to be shipped on U.S.-flag vessels... Maritime Administration, that (a) U.S.-flag vessels are not available at fair and reasonable rates for...

  15. 46 CFR 381.5 - Fix American-flag tonnage first.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... preference cargoes required by that Act and the Food Security Act of 1985 to be shipped on U.S.-flag vessels... Maritime Administration, that (a) U.S.-flag vessels are not available at fair and reasonable rates for...

  16. 46 CFR 381.5 - Fix American-flag tonnage first.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... preference cargoes required by that Act and the Food Security Act of 1985 to be shipped on U.S.-flag vessels... Maritime Administration, that (a) U.S.-flag vessels are not available at fair and reasonable rates for...

  17. Canine differential leukocyte counting with the CellaVision DM96Vision, Sysmex XT-2000iV, and Advia 2120 hematology analyzers and a manual method.

    PubMed

    Tvedten, Harold W; Lilliehöök, Inger E

    2011-09-01

    For differential leukocyte counts, automated blood smear evaluation systems have been too slow or inaccurate to replace or supplement the manual differential count. The CellaVision DM96Vision (DM96V), a new instrument, is an automated image analysis system that is rapid and accurate enough to be used for enumerating human leukocytes and may be useful for analysis of canine blood. The aims of this study were to evaluate the performance of the DM96V in differential counting of canine leukocytes, to compare its performance with that of other methods, and to analyze interoperator variability. Four methods of determining the leukocyte differential count of 108 canine blood samples were compared based on agreement, precision, and errors as well as relative performance. Differential counts were obtained using the DM96V, the manual method, and automated methods performed by the Advia 2120 and Sysmex XT-2000iV. All leukocyte types were detected by the DM96V and the manual method, and all 4 methods had similar mean and median results in most cases. The automated methods were more precise than either the DM96V or manual method when comparing identification of a single type of leukocyte, especially neutrophils and lymphocytes. However, precision of the automated methods was only fair for monocytes, and the Advia and Sysmex failed to identify basophils. The Advia reported fewer monocytes and eosinophils than did the other methods. Significantly fewer lymphocytes were identified by the manual method than by the Sysmex, Advia, and DM96V. The DM96V occasionally presented duplicate images of the same neutrophils. The CellaVision DM96V is a satisfactory system for facilitating canine differential leukocyte counting. The DM96V differential count was more similar to the manual count than to automated counts, which were more precise but had errors and omissions in detecting some types of leukocytes. ©2011 American Society for Veterinary Clinical Pathology.

  18. 46 CFR 381.9 - Available U.S.-flag service.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 8 2011-10-01 2011-10-01 false Available U.S.-flag service. 381.9 Section 381.9 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION MISCELLANEOUS CARGO PREFERENCE-U.S.-FLAG VESSELS § 381.9 Available U.S.-flag service. For purposes of shipping bulk agricultural commodities...

  19. 14 CFR 121.395 - Aircraft dispatcher: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Aircraft dispatcher: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Airman and Crewmember Requirements § 121.395 Aircraft dispatcher: Domestic and flag operations. Each certificate holder conducting domestic...

  20. 14 CFR 121.687 - Dispatch release: Flag and domestic operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Dispatch release: Flag and domestic... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.687 Dispatch release: Flag and domestic operations. (a) The dispatch release may be in any form but must contain...

  1. 14 CFR 121.541 - Operations schedules: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Operations schedules: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Operations § 121.541 Operations schedules: Domestic and flag operations. In establishing flight operations schedules, each...

  2. 14 CFR 121.687 - Dispatch release: Flag and domestic operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Dispatch release: Flag and domestic... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.687 Dispatch release: Flag and domestic operations. (a) The dispatch release may be in any form but must contain...

  3. 14 CFR 121.569 - Equipment interchange: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Equipment interchange: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Operations § 121.569 Equipment interchange: Domestic and flag operations. (a) Before operating under an interchange agreement,...

  4. 14 CFR 121.395 - Aircraft dispatcher: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Aircraft dispatcher: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Airman and Crewmember Requirements § 121.395 Aircraft dispatcher: Domestic and flag operations. Each certificate holder conducting domestic...

  5. 14 CFR 121.711 - Communication records: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Communication records: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.711 Communication records: Domestic and flag operations. Each certificate holder conducting domestic or...

  6. 14 CFR 121.541 - Operations schedules: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Operations schedules: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Operations § 121.541 Operations schedules: Domestic and flag operations. In establishing flight operations schedules, each...

  7. 14 CFR 121.569 - Equipment interchange: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Equipment interchange: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Operations § 121.569 Equipment interchange: Domestic and flag operations. (a) Before operating under an interchange agreement,...

  8. 14 CFR 121.711 - Communication records: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Communication records: Domestic and flag... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.711 Communication records: Domestic and flag operations. Each certificate holder conducting domestic or...

  9. 38 CFR 1.9 - Description, use, and display of VA seal and flag.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... in its talons. The cord binds a 13-star U.S. flag and a 50-star U.S. flag. In the field over the eagle is a pentagon formation of stars, with one point down. The words Department of Veterans Affairs and United States of America surround the eagle, stars, and flags. A rope motif makes up the...

  10. 38 CFR 1.9 - Description, use, and display of VA seal and flag.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... in its talons. The cord binds a 13-star U.S. flag and a 50-star U.S. flag. In the field over the eagle is a pentagon formation of stars, with one point down. The words Department of Veterans Affairs and United States of America surround the eagle, stars, and flags. A rope motif makes up the...

  11. 38 CFR 1.9 - Description, use, and display of VA seal and flag.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... in its talons. The cord binds a 13-star U.S. flag and a 50-star U.S. flag. In the field over the eagle is a pentagon formation of stars, with one point down. The words Department of Veterans Affairs and United States of America surround the eagle, stars, and flags. A rope motif makes up the...

  12. 10 CFR 1002.3 - Custody of official seal and distinguishing flags.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Custody of official seal and distinguishing flags. 1002.3 Section 1002.3 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG General § 1002.3 Custody of official seal and distinguishing flags. The Secretary or his designee...

  13. 10 CFR 1002.3 - Custody of official seal and distinguishing flags.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Custody of official seal and distinguishing flags. 1002.3 Section 1002.3 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG General § 1002.3 Custody of official seal and distinguishing flags. The Secretary or his designee...

  14. 10 CFR 1002.31 - Unauthorized uses of the seal and flag.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Unauthorized uses of the seal and flag. 1002.31 Section 1002.31 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Unauthorized Uses § 1002.31 Unauthorized uses of the seal and flag. The official seal and distinguishing...

  15. 10 CFR 1002.31 - Unauthorized uses of the seal and flag.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Unauthorized uses of the seal and flag. 1002.31 Section 1002.31 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Unauthorized Uses § 1002.31 Unauthorized uses of the seal and flag. The official seal and distinguishing...

  16. 10 CFR 1002.31 - Unauthorized uses of the seal and flag.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Unauthorized uses of the seal and flag. 1002.31 Section 1002.31 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Unauthorized Uses § 1002.31 Unauthorized uses of the seal and flag. The official seal and distinguishing...

  17. 10 CFR 1002.3 - Custody of official seal and distinguishing flags.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Custody of official seal and distinguishing flags. 1002.3 Section 1002.3 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG General § 1002.3 Custody of official seal and distinguishing flags. The Secretary or his designee...

  18. 10 CFR 1002.31 - Unauthorized uses of the seal and flag.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Unauthorized uses of the seal and flag. 1002.31 Section 1002.31 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Unauthorized Uses § 1002.31 Unauthorized uses of the seal and flag. The official seal and distinguishing...

  19. 10 CFR 1002.31 - Unauthorized uses of the seal and flag.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Unauthorized uses of the seal and flag. 1002.31 Section 1002.31 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG Unauthorized Uses § 1002.31 Unauthorized uses of the seal and flag. The official seal and distinguishing...

  20. 10 CFR 1002.3 - Custody of official seal and distinguishing flags.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Custody of official seal and distinguishing flags. 1002.3 Section 1002.3 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG General § 1002.3 Custody of official seal and distinguishing flags. The Secretary or his designee...

  1. 10 CFR 1002.3 - Custody of official seal and distinguishing flags.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Custody of official seal and distinguishing flags. 1002.3 Section 1002.3 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) OFFICIAL SEAL AND DISTINGUISHING FLAG General § 1002.3 Custody of official seal and distinguishing flags. The Secretary or his designee...

  2. 46 CFR 252.22 - Substantiality and extent of foreign-flag competition.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Substantiality and extent of foreign-flag competition... WORLDWIDE SERVICES Operation § 252.22 Substantiality and extent of foreign-flag competition. (a) Type and tonnage groupings. Foreign-flag competition shall be determined, as of January 1 of the year preceding...

  3. 46 CFR 154.17 - U.S. flag vessel: Certificate of Inspection endorsement.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false U.S. flag vessel: Certificate of Inspection endorsement... U.S. flag vessel: Certificate of Inspection endorsement. The Certificate of Inspection for a U.S. flag vessel allowed to carry a liquefied gas listed in Table 4 has the following endorsement for each...

  4. 48 CFR 52.247-63 - Preference for U.S.-Flag Air Carriers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Preference for U.S.-Flag... Clauses 52.247-63 Preference for U.S.-Flag Air Carriers. As prescribed in 47.405, insert the following clause: Preference for U.S.-Flag Air Carriers (JUN 2003) (a) Definitions. As used in this clause...

  5. 46 CFR 154.1801 - Certificates, letters, and endorsements: U.S. flag vessels.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Certificates, letters, and endorsements: U.S. flag... Operations § 154.1801 Certificates, letters, and endorsements: U.S. flag vessels. No person may operate a U.S. flag vessel unless the vessel has a Certificate of Inspection, issued under Subchapter D of this...

  6. 46 CFR 154.15 - U.S. flag vessel: Endorsement application.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false U.S. flag vessel: Endorsement application. 154.15.... flag vessel: Endorsement application. (a) A person who desires the endorsement required under § 154.1801 for a U.S. flag vessel must submit an application for an endorsement of the vessel's Subchapter D...

  7. 46 CFR 381.9 - Available U.S.-flag service.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Available U.S.-flag service. 381.9 Section 381.9 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION MISCELLANEOUS CARGO PREFERENCE-U.S.-FLAG VESSELS § 381.9 Available U.S.-flag service. For purposes of shipping bulk agricultural commodities under...

  8. 46 CFR 381.5 - Fix American-flag tonnage first.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Fix American-flag tonnage first. 381.5 Section 381.5 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION MISCELLANEOUS CARGO PREFERENCE-U.S.-FLAG VESSELS § 381.5 Fix American-flag tonnage first. Each department or agency having responsibility under the...

  9. 46 CFR 252.22 - Substantiality and extent of foreign-flag competition.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 8 2011-10-01 2011-10-01 false Substantiality and extent of foreign-flag competition... WORLDWIDE SERVICES Operation § 252.22 Substantiality and extent of foreign-flag competition. (a) Type and tonnage groupings. Foreign-flag competition shall be determined, as of January 1 of the year...

  10. 76 FR 20080 - Inventory of U.S.-Flag Launch Barges

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-11

    ... Maritime Administration Inventory of U.S.-Flag Launch Barges AGENCY: Maritime Administration, Department of Transportation. ACTION: Inventory of U.S.-Flag Launch Barges. SUMMARY: The Maritime Administration is updating its inventory of U.S.- flag launch barges. Additions, changes and comments to the list are...

  11. 77 FR 20098 - Inventory of U.S.-Flag Launch Barges

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-03

    ... Maritime Administration Inventory of U.S.-Flag Launch Barges AGENCY: Maritime Administration, Department of Transportation. ACTION: Inventory of U.S.-Flag Launch Barges. SUMMARY: The Maritime Administration is updating its inventory of U.S.- flag launch barges. Additions, changes and comments to the list are...

  12. 75 FR 13645 - Inventory of U.S.-Flag Launch Barges

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-22

    ... Maritime Administration Inventory of U.S.-Flag Launch Barges AGENCY: Maritime Administration, Department of Transportation. ACTION: Inventory of U.S.-Flag Launch Barges. SUMMARY: The Maritime Administration is updating its inventory of U.S.- flag launch barges. Additions, changes and comments to the list are...

  13. 14 CFR 121.687 - Dispatch release: Flag and domestic operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Dispatch release: Flag and domestic... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.687 Dispatch release: Flag and domestic operations. (a) The dispatch release may be in any form but must contain...

  14. 14 CFR 121.687 - Dispatch release: Flag and domestic operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Dispatch release: Flag and domestic... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.687 Dispatch release: Flag and domestic operations. (a) The dispatch release may be in any form but must contain...

  15. 14 CFR 121.615 - Dispatch or flight release over water: Flag and supplemental operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Dispatch or flight release over water: Flag...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.615 Dispatch or flight release over water: Flag and supplemental...

  16. 14 CFR 121.615 - Dispatch or flight release over water: Flag and supplemental operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Dispatch or flight release over water: Flag...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.615 Dispatch or flight release over water: Flag and supplemental...

  17. 14 CFR 121.615 - Dispatch or flight release over water: Flag and supplemental operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Dispatch or flight release over water: Flag...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.615 Dispatch or flight release over water: Flag and supplemental...

  18. 14 CFR 121.615 - Dispatch or flight release over water: Flag and supplemental operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Dispatch or flight release over water: Flag...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.615 Dispatch or flight release over water: Flag and supplemental...

  19. 14 CFR 121.687 - Dispatch release: Flag and domestic operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Dispatch release: Flag and domestic... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.687 Dispatch release: Flag and domestic operations. (a) The dispatch release may be in any form but must contain...

  20. 14 CFR 121.615 - Dispatch or flight release over water: Flag and supplemental operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Dispatch or flight release over water: Flag...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.615 Dispatch or flight release over water: Flag and supplemental...

  1. Reference genes for gene expression studies in wheat flag leaves grown under different farming conditions

    PubMed Central

    2011-01-01

    Background Internal control genes with highly uniform expression throughout the experimental conditions are required for accurate gene expression analysis as no universal reference genes exists. In this study, the expression stability of 24 candidate genes from Triticum aestivum cv. Cubus flag leaves grown under organic and conventional farming systems was evaluated in two locations in order to select suitable genes that can be used for normalization of real-time quantitative reverse-transcription PCR (RT-qPCR) reactions. The genes were selected among the most common used reference genes as well as genes encoding proteins involved in several metabolic pathways. Findings Individual genes displayed different expression rates across all samples assayed. Applying geNorm, a set of three potential reference genes were suitable for normalization of RT-qPCR reactions in winter wheat flag leaves cv. Cubus: TaFNRII (ferredoxin-NADP(H) oxidoreductase; AJ457980.1), ACT2 (actin 2; TC234027), and rrn26 (a putative homologue to RNA 26S gene; AL827977.1). In addition of these three genes that were also top-ranked by NormFinder, two extra genes: CYP18-2 (Cyclophilin A, AY456122.1) and TaWIN1 (14-3-3 like protein, AB042193) were most consistently stably expressed. Furthermore, we showed that TaFNRII, ACT2, and CYP18-2 are suitable for gene expression normalization in other two winter wheat varieties (Tommi and Centenaire) grown under three treatments (organic, conventional and no nitrogen) and a different environment than the one tested with cv. Cubus. Conclusions This study provides a new set of reference genes which should improve the accuracy of gene expression analyses when using wheat flag leaves as those related to the improvement of nitrogen use efficiency for cereal production. PMID:21951810

  2. HPC enumeration with the Sysmex XE-2100 can guide further flow cytometric CD34(+) measurements and timing of leukaphereses.

    PubMed

    Oelschlaegel, U; Bornhaeuser, M; Thiede, C; Ehninger, G; Hoelig, K

    2003-01-01

    The aim of this study was to evaluate whether HPC counts measured with the hematology analyzer can predict CD34+ levels in peripheral blood and in the apheresis product, as detected by standard flow cytometry. The main focus was the evaluation of HPC counts in poor mobilizers. Progenitor cell quantification was performed measuring HPC counts provided by the Sysmex XE-2100 hematology analyzer and CD34+ counts obtained in parallel by flow cytometry. Peripheral blood of patients who had received chemotherapy and G-CSF (142 measurements) and healthy donors mobilized with G-CSF alone (106 measurements) was investigated HPC counts in peripheral blood were also correlated with apheresis yield. HPC counts were significantly higher than CD34+ counts (3.5 fold inpatients and 1.7 fold in healthy donors, p= 0.0015). Our data indicate that HPC counts < or = 10/microL in pretreated patients predict a low probability of adequate CD34+ counts in peripheral blood and yields < 2 x 10(6)/kg in subsequent aphereses. Furthermore, repetitive low HPC enumerations in an individual were followed by insufficientCD34+ counts in peripheral blood or aphereses in 81% of investigations. In healthy donors low HPC counts (< or = 10/microL; 12/106 measurements) did not exclusively predict low CD34+ counts (median 23/microL). HPC counts can be used to schedule the start of CD34+ measurements(threshold > 10 HPC/microL) in patients mobilized after chemotherapy for autologous donation. Thus, expensive and time-consuming CD34+ enumerations can perhaps be minimized HPC measurements cannot completely replace flow cytometric CD34+ enumeration. In particular healthy stem-cell donors should be monitored with both methods to exclude false negative HPC measurements.

  3. Workers painting the Flag and Meatball on the VAB

    NASA Image and Video Library

    2007-01-03

    Elevated platforms are seen hanging in front of the NASA Logo on the side of Kennedy Space Center's Vehicle Assembly Building. Also in view on the east side of the building are platforms on the facility's large vertical doors. Workers, suspended on the platforms from the top of the 525-foot-high VAB, use rollers and brushes to do the painting. The flag and logo were last painted in 1998, honoring NASA's 40th anniversary. The flag spans an area 209 feet by 110 feet, or about 23, 437 square feet. Each stripe is 9 feet wide and each star is 6 feet in diameter. The logo, also known as the "meatball," measures 110 feet by 132 feet, or about 12,300 square feet.

  4. Workers painting the Flag and Meatball on the VAB

    NASA Image and Video Library

    2007-01-03

    Elevated platforms are seen hanging on the side of Kennedy Space Center's Vehicle Assembly Building in a view looking across from the turn basin. To the right is the large external tank barge. Workers, suspended on the platforms from the top of the 525-foot-high VAB, use rollers and brushes to do the painting. The flag and logo were last painted in 1998, honoring NASA's 40th anniversary. The flag spans an area 209 feet by 110 feet, or about 23, 437 square feet. Each stripe is 9 feet wide and each star is 6 feet in diameter. The logo, also known as the "meatball," measures 110 feet by 132 feet, or about 12,300 square feet.

  5. On pseudo-Finsler manifolds of scalar flag curvature

    NASA Astrophysics Data System (ADS)

    Hrimiuc, Dragos

    2015-12-01

    Let (M, L) be a pseudo-Finsler manifold, ξ the geodesic spray vector field associated to the non-degenerate, 2-positively homogeneous Lagrangian L. In this paper we prove that (M, L) is of scalar flag curvature k if and only if the equation Lξ g + kλLξ g ˆ = 0 holds on Γ(Iλ M), the Lie algebra of tangent vector fields to the λ-indicatrix bundle Iλ M, where g and g ˆ are pseudo-Riemannian metrics on the vertical and respectively on the horizontal subbundle. Also, we prove that any pseudo-Finsler manifold is of scalar flag curvature at any point of the light cone.

  6. 10 CFR 1002.21 - Description of distinguishing flag.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... shall be of rayon banner, measure 4′4″ on hoist by 5′6″ on the fly, exclusive of heading and hems, and be fringed on three edges with yellow rayon fringe, 21/2″ wide. (2) The outdoor flag shall be of heavy weight nylon, and measure either 3′ on the hoist by 5′ on the fly or 5′ on the hoist by 8′ on...

  7. 10 CFR 1002.21 - Description of distinguishing flag.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... shall be of rayon banner, measure 4′4″ on hoist by 5′6″ on the fly, exclusive of heading and hems, and be fringed on three edges with yellow rayon fringe, 21/2″ wide. (2) The outdoor flag shall be of heavy weight nylon, and measure either 3′ on the hoist by 5′ on the fly or 5′ on the hoist by 8′ on...

  8. 10 CFR 1002.21 - Description of distinguishing flag.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... shall be of rayon banner, measure 4′4″ on hoist by 5′6″ on the fly, exclusive of heading and hems, and be fringed on three edges with yellow rayon fringe, 21/2″ wide. (2) The outdoor flag shall be of heavy weight nylon, and measure either 3′ on the hoist by 5′ on the fly or 5′ on the hoist by 8′ on...

  9. 10 CFR 1002.21 - Description of distinguishing flag.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... shall be of rayon banner, measure 4′4″ on hoist by 5′6″ on the fly, exclusive of heading and hems, and be fringed on three edges with yellow rayon fringe, 21/2″ wide. (2) The outdoor flag shall be of heavy weight nylon, and measure either 3′ on the hoist by 5′ on the fly or 5′ on the hoist by 8′ on...

  10. When Does the Coast Guard’s Flag Come Forward

    DTIC Science & Technology

    2007-11-02

    WHEN DOES THE COAST GUARD’S FLAG COME FORWARD? A thesis presented to the Faculty of the U.S. Army Command and General Staff College...are those of the student author and do not necessarily represent the views of the U.S. Army Command and General Staff College or any other...leaders and the general public. In turn, this may lead to an increased budget that will permit the Coast Guard to operate more effectively, providing even

  11. Diagnostic performance of the variant lymphocyte flag of the Abbott Cell-Dyn 4000 haematology analyser.

    PubMed

    Hoffmann, J J M L; Hoedemakers, R M J

    2004-02-01

    In addition to differential cell counts, modern haematology analysers generate suspect flags if abnormal cells are detected. Reports on validation of suspect flags are scarce. We have routine experience with the Abbott Cell-Dyn 4000 analyser for over 5 years and have previously demonstrated the utility of the blast flag. Here we report a similar study on the performance of the analyser's Variant Lymphocyte (VL) flag. Evaluation of the diagnostic performance of the Cell-Dyn 4000 VL flag, as compared with lymphocyte morphology in blood smears. In addition, we investigated the usefulness of the numerical VL flag confidence index as provided by the analyser. All samples generating a VL flag were reviewed over a 5-month period. We also reviewed smears from patients with known lymphoid disorders, even if the analyser did not flag the sample. Two experienced investigators assessed lymphocyte morphology independently. In total, 187 samples were included in the study, of which 183 had a VL flag and four had not. Of the 183 flagged samples, 83 appeared to have abnormal lymphocyte morphology and 100, normal lymphocyte morphology. The sensitivity of the VL flag for detecting abnormal lymphocytes was 0.95 and the positive predictive value was 0.44. Using ROC analysis of the VL flag confidence index, the area under the ROC curve was 0.58 (95% confidence interval 0.50-0.65). The Cell-Dyn VL flag has reasonable sensitivity but a high false-positive rate. In addition, its performance is insufficient for detecting clinically relevant abnormal lymphocytes. As the VL flag appeared to rely mainly on numerical criteria, it has no added value over numerical criteria defined by the laboratory.

  12. Cell Population Data and reflex testing rules of cell analysis in pleural and ascitic fluids using body fluid mode on Sysmex XN-9000.

    PubMed

    Buoro, Sabrina; Mecca, Tommaso; Azzarà, Giovanna; Seghezzi, Michela; Dominoni, Paola; Crippa, Alberto; Ottomano, Cosimo; Lippi, Giuseppe

    2016-01-15

    Although optical microscopy (OM) remains the reference technique for analysis of ascitic (AF) and pleural (PF) fluids, novel hematological analyzers are equipped with modules for body fluid (BF) analysis. This study was aimed to analyze the performance of XN-BF module in Sysmex XN-9000, and to develop validation rules for automated cell counts in BFs. The evaluation of XN-BF module included assessment of carryover, Limit of Blank (LoB), Limit of Detection (LoD), Limit of Quantitation (LoQ), linearity, data comparison with OM, and development of rules for assisting the validation of automated analysis of BFs and activating reflex testing. The carryover was negligible. The LoB, LoD, LoQ and linearity were always excellent. The comparison with OM was characterized by Pearson's correlations ranging from r=0.50 to r=0.99 (p<0.001), modest bias and high diagnostic concordance (Area Under the Curve between 0.85 and 0.99). The use of instrument-specific cut-offs further increased diagnostic concordance. The implementation of reflex testing rules based on XN-BF data increased sensitivity and specificity of BFs classification to 0.98 and 0.95. Our results suggest that the XN-BF module on Sysmex-9000 may be a suitable alternative to OM for screening BF samples, especially when specific validation rules are used. Copyright © 2015 Elsevier B.V. All rights reserved.

  13. Detection of malignant cells in serous body fluids by counting high-fluorescent cells on the Sysmex XN-2000 hematology analyzer.

    PubMed

    Labaere, D; Boeckx, N; Geerts, I; Moens, M; Van den Driessche, M

    2015-10-01

    The body fluid mode of the Sysmex XN-2000 hematology analyzer differentiates cells into mononuclear and polymorphonuclear white blood cells (WBC) and high-fluorescent cells (HFC). The aim of this study was to evaluate the performance of the HFC count for detecting malignant cells in serous body fluids. Two-hundred and thirty serous fluids were analyzed on the Sysmex XN body fluid mode. HFC were measured as relative count (HFC/100 WBC) and absolute count (HFC/μL). All samples were microscopically screened on cytospin slides for the presence of malignant cells. Malignant cells were found by microscopic examination in 49 of 230 samples (21.3%). Malignant samples contained significantly higher percentages (10.2 vs. 2.6/100 WBC) and absolute numbers (65 vs. 10/μL) of HFC than nonmalignant samples (P < 0.001). Areas under the ROC curve for relative and absolute HFC count were 0.69 and 0.77, respectively. A cutoff level of ≥17 HFC/μL showed the best performance to predict malignancy, with 88% sensitivity and 61% specificity. As serous body fluids will be more analyzed on automated analyzers in the future, HFC count can be a useful tool to select samples for microscopic review. Microscopic evaluation should be performed if HFC values are above a certain threshold (e.g. ≥17 HFC/μL) or in case of clinical suspicion of malignancy. © 2015 John Wiley & Sons Ltd.

  14. Vortex dynamics and flapping patterns of the inverted flag with a bluff body

    NASA Astrophysics Data System (ADS)

    Kim, Hyeonseong; Kim, Junyoung; Kim, Daegyoum

    2015-11-01

    Flow-induced vibration of flexible structures for energy harvesting has drawn attention recently. The inverted flag whose trailing edge is clamped and leading edge is free to move was known to self-excite in a uniform flow of both water and air. In this study, we investigated the effect of an upstream bluff body, a flat plate, on the dynamics of the downstream inverted flag. Periodic vortical structures created by an upstream bluff body make the dynamics of the inverted flag quite different from those of the inverted flag without the bluff body. We examined the motion of the inverted flag by varying the relative displacement of the inverted flag from the bluff body and their relative size. Our results show that the inverted flag can flap with higher frequency and larger amplitude with the upstream bluff body. We also compared the dynamics of the inverted flag with those of the general flag with the upstream bluff body. In order to better understand the dynamics of the flag, the analysis of the flow patterns using particle image velocimetry was also conducted.

  15. Most red flags for malignancy in low back pain guidelines lack empirical support: a systematic review.

    PubMed

    Verhagen, Arianne P; Downie, Aron; Maher, Chris G; Koes, Bart W

    2017-10-01

    Clinicians do not want to miss underlying serious pathology, but it is still unclear which red flags are relevant. We aimed to evaluate the origin and evidence on diagnostic accuracy of red flags for malignancy for management of low back pain (LBP) in primary care. We performed a comprehensive overview and searched the literature using snowballing techniques and reference checking for evidence on red flags endorsed in clinical guidelines for identifying patients with higher likelihood of malignancy. We selected studies including people with LBP without any restriction on study design. We extracted data on prevalence and diagnostic accuracy. Furthermore, we assessed the methodological quality of studies evaluating diagnostic accuracy. We identified 13 red flags endorsed in a total of 16 guidelines and 2 extra red flags not endorsed in any guideline. We included 33 publications varying from systematic reviews to case reports. The origin of many red flags was unclear or was sourced from case reports. The incidence of malignancy in patients presenting with LBP in primary care varied between 0% and 0.7%. Seven studies provided diagnostic accuracy data on red flags. We found 5 red flags with accuracy data from 2 or more studies, with 2 ("history of malignancy" and "strong clinical suspicion") considered informative. In conclusion, the origin and diagnostic accuracy of many red flags endorsed in guidelines are unclear. A "history of malignancy" and "strong clinical suspicion" are the only red flags with empirical evidence of acceptably high diagnostic accuracy.

  16. Evaluation of Sysmex XN-1000 hematology analyzer for cell count and screening of malignant cells of serous cavity effusion

    PubMed Central

    Xu, Weiyi; Yu, Qian; Xie, Lixia; Chen, Baode; Zhang, Ling

    2017-01-01

    Abstract Over the years, with the advancement in hematology analyzer technology, the use of fluid analysis method has seen a drastic increase in clinical examinations. Cell counting and classification in independent body fluid analysis method are conducted by semiconductor laser flow cytometry and nucleic acid fluorescence staining techniques. This study is to evaluate the efficacy of Sysmex XN-1000 hematology analyzer in cell counting and to screen malignant cells with serous cavity effusion. Specimens (N = 206) with serous cavity effusion from our hospital were included in this study. Manual and instrumental methods for cell counting, nucleated cell classification, and high-fluorescent cells (HFC) were used in this study. The correlation between RBC, nucleated cell count (NUC), the percentages of polymorphonuclear cell (PMN%), and mononuclear cells (MN%) was statistically analyzed using manual and instrumental methods. The regression equations of RBC, NUC, PMN%, and MN% in the manual and instrumental methods were RBC y = 0.88x + 426.4; NUC y = 0.85x + 33.4; PMN% y = 0.91x + 4.2; and MN% y = 0.91x + 5.1. Correlation coefficient R2 was 0.99, 0.98, 0.90, and 0.90 (P < .001). ROC curve analysis showed that when the cut-off value of HFC% was 4.4% and HFC# was 24.5/μL, area under curve (AUC), sensitivity, specificity, and 95% confidence interval were 0.707, 0.792, 0.558, 0.637–0.777; 0.708, 0.753, 0.550, 0.635–0.780, respectively. XN-1000 hematology analyzer body fluid method can accurately and rapidly count cell and nucleated cell classification with serous cavity effusion. HFC can indicate the possible existence of malignant cells; however, further investigations are required to validate its efficacy. PMID:28682907

  17. THE FLAG: A Web Resource of Innovative Assessment Tools for Faculty in College Science, Mathematics, Engineering, and Technology

    NASA Astrophysics Data System (ADS)

    Zeilik, M.; Mathieu, R. D.; National InstituteScience Education; College Level-One Team

    2000-12-01

    Even the most dedicated college faculty often discover that their students fail to learn what was taught in their courses and that much of what students do learn is quickly forgotten after the final exam. To help college faculty improve student learning in college Science, Mathematics, Engineering and Technology (SMET), the College Level - One Team of the National Institute for Science Education has created the "FLAG" a Field-tested Learning Assessment Guide for SMET faculty. Developed with funding from the National Science Foundation, the FLAG presents in guidebook format a diverse and robust collection of field-tested classroom assessment techniques (CATs), with supporting information on how to apply them in the classroom. Faculty can download the tools and techniques from the website, which also provides a goals clarifier, an assessment primer, a searchable database, and links to additional resources. The CATs and tools have been reviewed by an expert editorial board and the NISE team. These assessment strategies can help faculty improve the learning environments in their SMET courses especially the crucial introductory courses that most strongly shape students' college learning experiences. In addition, the FLAG includes the web-based Student Assessment of Learning Gains. The SALG offers a convenient way to evaluate the impact of your courses on students. It is based on findings that students' estimates of what they gained are more reliable and informative than their observations of what they liked about the course or teacher. It offers accurate feedback on how well the different aspects of teaching helped the students to learn. Students complete the SALG online after a generic template has been modified to fit the learning objectives and activities of your course. The results are presented to the teacher as summary statistics automatically. The FLAG can be found at the NISE "Innovations in SMET Education" website at www.wcer.wisc.edu/nise/cl1

  18. Astronaut Returns Space-Flown Flag to NRAO

    NASA Astrophysics Data System (ADS)

    2002-04-01

    A NASA Astronaut who carried a flag bearing the logo of the National Radio Astronomy Observatory (NRAO) on last month's Shuttle flight returned that flag to the observatory on Friday, April 12, at a ceremony in Socorro. Dr. John M. Grunsfeld, payload commander of the STS-109 flight, also addressed students at the New Mexico State Science Fair on Saturday, April 13, on the campus of New Mexico Tech in Socorro. Dr. John M. Grunsfeld: Click on image for larger view Dr. John M. Grunsfeld Grunsfeld, an astronomer who observed with NRAO's Very Large Array (VLA) radio telescope in 1991, carried the 3-by-5-foot NRAO flag aboard Columbia during the March 1-12 mission to service the Hubble Space Telescope. The NRAO flag, made by Socorro resident Dora Spargo, had been sent to the observatory's facilities in Socorro; Tucson, AZ; Green Bank, WV; and Charlottesville, VA, where NRAO employees signed it prior to the flight. "With our signatures on that flag, we all felt like we were riding along with John aboard the Shuttle," said NRAO Director Paul Vanden Bout. "We are proud that NRAO was represented on a mission that was so important to astronomy," Vanden Bout added. During the flight, Grunsfeld performed three of the five spacewalks in which crew members successfully upgraded the Hubble Space Telescope. The astronauts left the space observatory with a new power unit, a new camera and new solar arrays. The upgrades, said Grunsfeld, leave the orbiting telescope with "its discovery potential significantly increased." STS-109 was Grunsfeld's fourth space flight and his second visit to Hubble. A research astronomer who received his bachelor's degree in physics from MIT and a Ph.D from the University of Chicago in 1988, Grunsfeld was selected to the astronaut corps in 1992. His first space flight, in 1995, featured astronomical observations using the Astro observatory, a three-telescope facility aboard the Shuttle Endeavour. In 1997, Grunsfeld rode Atlantis on a 10-day mission to the

  19. The epidemiology of injuries in contact flag football.

    PubMed

    Kaplan, Yonatan; Myklebust, Grethe; Nyska, Meir; Palmanovich, Ezequiel; Victor, Jan; Witvrouw, Erik

    2013-01-01

    To characterize the epidemiology of injuries in post-high school male and female athletes in the rapidly growing international sport of contact flag football. Prospective injury-observational study. Kraft Stadium, Jerusalem, Israel. A total of 1492 players, consisting of men (n = 1252, mean age, 20.49 ± 5.11) and women (n = 240, mean age, 21.32 ± 8.95 years), participated in 1028 games over a 2-season period (2007-2009). All time-loss injuries sustained in game sessions were recorded by the off-the-field medical personnel and followed up by a more detailed phone injury surveillance questionnaire. One hundred sixty-three injuries were reported, comprising 1 533 776 athletic exposures (AEs). The incidence rate was 0.11 [95% confidence interval (CI), 0.09-0.12] per 1000 AEs, and incidence proportion was 10.66% (95% CI, 9.10-12.22). Seventy-six percent of the injuries were extrinsic in nature. Thirty percent of the injuries were to the fingers, thumb, and wrist, 17% to the knee, 17% to the head/face, 13% to the ankle, and 11% to the shoulder. Contact flag football results in a significant amount of moderate to severe injuries. These data may be used in the development of a formal American flag football injury database and in the development and implementation of a high-quality, randomized, prospective injury prevention study. This study should include the enforcement of the no-pocket rule, appropriate headgear, self-fitting mouth guards, the use of ankle braces, and changing the blocking rules of the game.

  20. The US Flag Merchant Marine: A National Asset?

    DTIC Science & Technology

    1994-06-03

    freight imported or exported in American vessels accrues to American capital, and the enterprise is as much a productive industry as the raising of...and yet in 1991, when 829.1 million tons of cargo passed through American ports, only 4%, 33.4 million tons was carried in U.S. flagged ships. 2 In...fact, in 1988, merchant ships of the former Soviet Union carried more American cargo than did U.S. ships, including nearly 50% of the outbound U.S. mail

  1. Astronaut John Young leaps from lunar surface to salute flag

    NASA Technical Reports Server (NTRS)

    1972-01-01

    Astronaut John W. Young, commander of the Apollo 16 lunar landing mission, leaps from the lunar surface as he salutes the U.S. Flag at the Descartes landing site during the first Apollo 16 extravehicular activity (EVA-1). Astronaut Charles M. Duke Jr., lunar module pilot, took this picture. The Lunar Module (LM) 'Orion' is on the left. The Lunar Roving Vehicle is parked beside the LM. The object behind Young in the shade of the LM is the Far Ultraviolet Camera/Spectrograph. Stone Mountain dominates the background in this lunar scene.

  2. Algorithms on Flag Manifolds for Knowledge Discovery in N-way Arrays

    DTIC Science & Technology

    2015-11-20

    AFRL-AFOSR-VA-TR-2015-0390 Algorithms on Flag Manifolds for Knowledge Discovery in N-way Arrays Michael Kirby COLORADO STATE UNIVERSITY Final Report...TYPE Final Performance 3. DATES COVERED (From - To) 01-08-2012 to 31-07-2015 4. TITLE AND SUBTITLE Algorithms on Flag Manifolds for Knowledge Discovery...livelink.ebs.afrl.af.mil/livelink/llisapi.dll DISTRIBUTION A: Distribution approved for public release Final Report Algorithms on Flag Manifolds for Knowledge Discovery in

  3. Dynamic Black-Level Correction and Artifact Flagging for Kepler Pixel Time Series

    NASA Technical Reports Server (NTRS)

    Kolodziejczak, J. J.; Clarke, B. D.; Caldwell, D. A.

    2011-01-01

    Methods applied to the calibration stage of Kepler pipeline data processing [1] (CAL) do not currently use all of the information available to identify and correct several instrument-induced artifacts. These include time-varying crosstalk from the fine guidance sensor (FGS) clock signals, and manifestations of drifting moire pattern as locally correlated nonstationary noise, and rolling bands in the images which find their way into the time series [2], [3]. As the Kepler Mission continues to improve the fidelity of its science data products, we are evaluating the benefits of adding pipeline steps to more completely model and dynamically correct the FGS crosstalk, then use the residuals from these model fits to detect and flag spatial regions and time intervals of strong time-varying black-level which may complicate later processing or lead to misinterpretation of instrument behavior as stellar activity.

  4. Swimming between the flags: a preliminary exploration of the influences on Australians' intentions to swim between the flags at patrolled beaches.

    PubMed

    White, Katherine M; Hyde, Melissa K

    2010-11-01

    Swimming at patrolled beaches reduces the likelihood of drownings and near-drownings. The present study tested the Theory of Planned Behaviour (TPB), with the addition of risk perceptions, in predicting people's intentions to swim between the flags at patrolled beaches. We examined also the predictors of people's willingness to swim [1] up to 10 m and [2] more than 10 m outside of the patrol flags. Participants (N=526) completed measures of attitudes, subjective norm, perceived behavioural control (PBC), intentions/willingness, and both objective and subjective risk perceptions. Two weeks later, a sub-sample of participants reported on their beach swimming behaviour for the previous fortnight. Attitude and subjective norm predicted intentions to swim between and willingness to swim outside of the flags. Age and PBC influenced willingness to swim beyond the flags. Objective risk predicted willingness to swim beyond the flags (both distances) while subjective risk predicted willingness to swim up to 10 m outside the flags. People's intentions to swim between the flags were correlated with their behaviour at follow-up. This study provides a preliminary investigation into an important safety behaviour and identifies factors to target when promoting safe swimming behaviours to prevent drowning deaths on Australian beaches.

  5. 75 FR 26645 - Operating Requirements: Domestic, Flag, Supplemental, Commuter, and On-Demand Operations...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ..., Supplemental, Commuter, and On-Demand Operations: Corrections and Editorial Changes AGENCY: Federal Aviation... Requirements: Domestic, Flag, Supplemental, Commuter, and On-Demand Operations: Corrections and Editorial...

  6. 14 CFR 121.637 - Takeoffs from unlisted and alternate airports: Domestic and flag operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and... dispatched according to dispatching rules applicable to operation from an approved airport; and (4)...

  7. 14 CFR 121.637 - Takeoffs from unlisted and alternate airports: Domestic and flag operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and... dispatched according to dispatching rules applicable to operation from an approved airport; and (4)...

  8. 14 CFR 121.637 - Takeoffs from unlisted and alternate airports: Domestic and flag operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and... dispatched according to dispatching rules applicable to operation from an approved airport; and (4)...

  9. Riemannian Optimization Method on Generalized Flag Manifolds for Complex and Subspace ICA

    NASA Astrophysics Data System (ADS)

    Nishimori, Yasunori; Akaho, Shotaro; Plumbley, Mark D.

    2006-11-01

    In this paper we introduce a new class of manifolds, generalized flag manifolds, for the complex and subspace ICA problems. A generalized flag manifold is a manifold consisting of subspaces which are orthogonal to each other. The class of generalized flag manifolds include the class of Grassmann manifolds. We extend the Riemannian optimization method to include this new class of manifolds by deriving the formulas for the natural gradient and geodesics on these manifolds. We show how the complex and subspace ICA problems can be solved by optimization of cost functions on a generalized flag manifold. Computer simulations demonstrate our algorithm gives good performance compared with the ordinary gradient descent method.

  10. Feline reference intervals for the Sysmex XT-2000iV and the ProCyte DX haematology analysers in EDTA and CTAD blood specimens.

    PubMed

    Granat, Fanny; Geffré, Anne; Bourgès-Abella, Nathalie; Mortier, Jeremy; Théron, Marie-Laure; Fauchon, Emilie; Braun, Jean-Pierre; Trumel, Catherine

    2014-06-01

    Laser-based haematology analysers are routinely used in veterinary clinical pathology laboratories, and are available to practitioners. However, feline haematological reference intervals (RIs) determined according to international recommendations are, to our knowledge, not available. Furthermore, platelet count RI is difficult to establish in cats because of the frequent occurrence of platelet aggregation in blood specimens. The purpose of this study was to establish feline haematological RIs with the Sysmex XT-2000iV and ProCyte DX analysers, in ethylenediamine tetra-acetic acid (EDTA) and in citrate, theophylline, adenosine and dipyridamole (CTAD), which is a combination of anticoagulants limiting platelet aggregation. Blood specimens from 120 healthy cats were analysed in duplicate, and the degree of platelet aggregation was assessed on blood smears. After exclusion of inadequate specimens, 81 sets of results (from 44 males and 37 females, aged from 6 to 116 months) were available for the determination of RIs by the non-parametric method. The effects of the anticoagulant, analyser and aggregation score were assessed. When the aggregation effect was significant, the RIs were determined using the subgroup of blood specimens with no or little aggregation. The effects of sex, age and weight were also investigated, but were moderate. The different RIs obtained with the Sysmex XT-2000iV and ProCyte DX analysers, and the two anticoagulants, were very similar to previous RIs established in EDTA with the ADVIA 120, another laser-based analyser, except for the platelet count in CTAD specimens. Its lower reference limit was higher in CTAD vs EDTA specimens, which confirms the interest in this anticoagulant in cats.

  11. Evaluation and optimization of the Sysmex UF1000i system for the screening of urinary tract infection in primary health care elderly patients.

    PubMed

    Martín-Gutiérrez, Guillermo; Porras-González, Ana; Martín-Pérez, Carlos; Lepe, Jose Antonio; Aznar, Javier

    2015-05-01

    Urinary tract infections (UTIs) are a common problem in the elderly population. Urine culture is still considered the "gold standard" to diagnose infection in this population. However, urine cultures are laborious and costly, and most samples will yield no growth. An evaluation was made of the Sysmex UF-1000i flow cytometer as a screening tool for UTI in an elderly population older than 65 years who lived in the community, using 346 urine samples submitted for culture. The Receiver Operating Characteristic (ROC) analysis showed a significant difference (P<0.01) between 0.98 bacteria area under the curve value and 0.82 of white blood cells (WBC). The combination of both counts for screening did not show any improvement in specificity or sensitivity. According to our data, the use of a single cut-off point of 200bacteria/μL is suggested, in which the sensitivity and specificity were 99.11% and 91.59%, respectively, with a NPV of 99.49%. Moreover, this cut-off value could avoid 60.24% of the samples to be cultured, with a minimal false negative results rate of 0.87%. The stratification of age groups stratification helps in selecting a more adjusted Sysmex UF1000i cut-off limit, leading to an improvement in the screening parameters that would imply a better management of these infections, as well as a high reduction in the workload and cost savings. Copyright © 2014 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

  12. Predicting and controlling risks from human exposures to vibration and mechanical shock: flag waving and flag weaving.

    PubMed

    Griffin, Michael J

    2015-01-01

    At work or in leisure activities, many people are exposed to vibration or mechanical shocks associated with risks of injury or disease. This paper identifies information that can be used to decide whether there may be a risk from exposure to hand-transmitted vibration or whole-body vibration and shock, and suggests actions that can control the risks. The complex and time-varying nature of human exposures to vibration and shock, the complexity of the different disorders and uncertainty as to the mechanisms of injury and the factors influencing injury have prevented the definition of dose-response relationships well proven by scientific study. It is necessary to wave a flag indicating when there is a need to control risks from exposure to vibration and shock while scientific enquiry provides understanding needed to weave a better flag. It is concluded that quantifying exposure severity is often neither necessary nor sufficient to either identify risks or implement measures that control the risks. The identification of risks associated with exposure to vibration and mechanical shock cannot, and need not, rely solely on the quantification of exposure severity. Qualitative methods can provide a sufficient indication of the need for control measures, which should not be restricted to reducing standardised measures of exposure severity.

  13. Reserve Component General and Flag Officers: A Review of Requirements and Authorized Strength

    DTIC Science & Technology

    2016-01-01

    Reserve Component General and Flag Offi cers A Review of Requirements and Authorized Strength Lisa M. Harrington, Igor Mikolic-Torreira, Geoffrey...trademark. iii Preface The Fiscal Year 2014 National Defense Authorization Act directed the Secretary of Defense to conduct a review of requirements for...26 CHAPTER FOUR Review of General and Flag Officer Positions

  14. 75 FR 67453 - Identity Theft Red Flags and Address Discrepancies Under the Fair and Accurate Credit...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-02

    ... Office of Thrift Supervision Identity Theft Red Flags and Address Discrepancies Under the Fair and...: Identity Theft Red Flags and Address Discrepancies under the Fair and Accurate Credit Transactions Act of... issue jointly guidelines for financial institutions and creditors regarding identity theft with...

  15. Astronaut Harrison Schmitt next to deployed U.S. flag on lunar surface

    NASA Technical Reports Server (NTRS)

    1972-01-01

    Scientist-Astronaut Harrison Schmitt, Apollo 17 lunar module pilot, is photographed next to the U.S. flag during extravehicular activity (EVA) of NASA's final lunar landing mission in the Apollo series. The photo was taken at the Taurus-Littrow landing site. The highest part of the flag appears to point toward our planet earth in the distant background.

  16. Mid deck in-orbit crew portrait taken in flag motif shirts.

    NASA Technical Reports Server (NTRS)

    1992-01-01

    Mid deck in orbit portraits of the entire crew taken in flag motif shirts. Flag motif jersey portraits - front row, left to right, Mission Specialists Kathy Thornton and Rick Heib, middlle row, left to right, Mission Specialists Pierre Thuot and Tom Akers, back row, left to right, Mission Commander Dan Brandenstein, Mission Pilot Kevin Chilton and Mission Specialist Bruce Melnick.

  17. FlagHouse Forum: You Say "Tomato"... and I Use a Communicator

    ERIC Educational Resources Information Center

    Exceptional Parent, 2011

    2011-01-01

    This month's "FlagHouse Forum" focuses on how to choose the communicator best-suited to a child's special need. FlagHouse--a premier global supplier of resources for special needs, education, physical activity and recreation--is pleased to partner with "Exceptional Parent" to bring its readers this informational forum. Humans communicate with each…

  18. 14 CFR 121.641 - Fuel supply: Nonturbine and turbo-propeller-powered airplanes: Flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Fuel supply: Nonturbine and turbo-propeller-powered airplanes: Flag operations. 121.641 Section 121.641 Aeronautics and Space FEDERAL AVIATION... Flight Release Rules § 121.641 Fuel supply: Nonturbine and turbo-propeller-powered airplanes: Flag...

  19. 14 CFR 121.641 - Fuel supply: Nonturbine and turbo-propeller-powered airplanes: Flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Fuel supply: Nonturbine and turbo-propeller-powered airplanes: Flag operations. 121.641 Section 121.641 Aeronautics and Space FEDERAL AVIATION... Flight Release Rules § 121.641 Fuel supply: Nonturbine and turbo-propeller-powered airplanes: Flag...

  20. 14 CFR 121.465 - Aircraft dispatcher duty time limitations: Domestic and flag operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Aircraft dispatcher duty time limitations: Domestic and flag operations. 121.465 Section 121.465 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Qualifications and Duty Time Limitations: Domestic and Flag Operations; Flight Attendant Duty Period Limitations...

  1. 14 CFR 121.465 - Aircraft dispatcher duty time limitations: Domestic and flag operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Aircraft dispatcher duty time limitations: Domestic and flag operations. 121.465 Section 121.465 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Qualifications and Duty Time Limitations: Domestic and Flag Operations; Flight Attendant Duty Period Limitations...

  2. 14 CFR 121.465 - Aircraft dispatcher duty time limitations: Domestic and flag operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Aircraft dispatcher duty time limitations: Domestic and flag operations. 121.465 Section 121.465 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Qualifications and Duty Time Limitations: Domestic and Flag Operations; Flight Attendant Duty Period Limitations...

  3. 14 CFR 121.637 - Takeoffs from unlisted and alternate airports: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... minimums for takeoff prescribed or approved by the government of the country in which the airport is... airports: Domestic and flag operations. 121.637 Section 121.637 Aeronautics and Space FEDERAL AVIATION...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and...

  4. 14 CFR 121.637 - Takeoffs from unlisted and alternate airports: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... minimums for takeoff prescribed or approved by the government of the country in which the airport is... airports: Domestic and flag operations. 121.637 Section 121.637 Aeronautics and Space FEDERAL AVIATION...: CERTIFICATION AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and...

  5. 46 CFR 154.19 - U.S. flag vessel: IMO certificate issuance.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES General § 154.19 U.S. flag vessel: IMO certificate issuance. (a) Either a classification society authorized under 46 CFR part... 46 Shipping 5 2012-10-01 2012-10-01 false U.S. flag vessel: IMO certificate issuance....

  6. 46 CFR 154.19 - U.S. flag vessel: IMO certificate issuance.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES General § 154.19 U.S. flag vessel: IMO certificate issuance. (a) Either a classification society authorized under 46 CFR part... 46 Shipping 5 2014-10-01 2014-10-01 false U.S. flag vessel: IMO certificate issuance....

  7. 46 CFR 154.19 - U.S. flag vessel: IMO certificate issuance.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES General § 154.19 U.S. flag vessel: IMO certificate issuance. (a) Either a classification society authorized under 46 CFR part... 46 Shipping 5 2011-10-01 2011-10-01 false U.S. flag vessel: IMO certificate issuance....

  8. 46 CFR 154.19 - U.S. flag vessel: IMO certificate issuance.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES General § 154.19 U.S. flag vessel: IMO certificate issuance. (a) Either a classification society authorized under 46 CFR part... 46 Shipping 5 2013-10-01 2013-10-01 false U.S. flag vessel: IMO certificate issuance....

  9. 46 CFR 154.19 - U.S. flag vessel: IMO certificate issuance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING BULK LIQUEFIED GASES General § 154.19 U.S. flag vessel: IMO certificate issuance. (a) Either a classification society authorized under 46 CFR part... 46 Shipping 5 2010-10-01 2010-10-01 false U.S. flag vessel: IMO certificate issuance....

  10. 14 CFR 121.711 - Communication records: Domestic and flag operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Communication records: Domestic and flag operations. 121.711 Section 121.711 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.711...

  11. 14 CFR 121.711 - Communication records: Domestic and flag operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Communication records: Domestic and flag operations. 121.711 Section 121.711 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.711...

  12. Enhancement of heat transfer by clamped flags in a Poiseuille channel flow

    NASA Astrophysics Data System (ADS)

    Lee, Jae Bok; Park, Sung Goon; Kim, Boyoung; Sung, Hyung Jin

    2016-11-01

    A pair of flexible flags clamped vertically in a heated channel was numerically modeled to study an enhancement of heat transfer by the clamped flags in a Poiseuille channel flow. The penalty immersed boundary method was adopted to analyze the fluid-structure-thermal interaction between the surrounding fluid and the clamped flags. The dynamics of the clamped flags was categorized into three distinctive modes: a flapping mode, a fully deflected mode, and an irregular mode. The distinctive modes that depended on the relationship between the hydrodynamic force and the restoring force displayed different movement patterns. The flapping mode provided superior thermal performance to the other modes. Vortices generated from the flapping flags swept out the thermal boundary layer and entrained the fluid near channel walls into the channel core flow while passing through the wake periodically. Compared to rigid flags, the flapping flags significantly improved the thermal efficiency. In addition, the effects of channel height and Reynolds number on the thermal efficiency were explored to obtain an optimal parameter set, which presented the highest thermal performance in present study. The flexible flags regarding the optimal parameter set showed an increase of up to 230% in net heat flux, compared to the baseline flow. Dynamic modes decomposition (DMD) method was adopted to examine the correlation between the vorticity and temperature fields.

  13. 40 CFR 161.34 - Flagging of studies for potential adverse effects.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Flagging of studies for potential... Provisions § 161.34 Flagging of studies for potential adverse effects. (a) Any person who submits a study of... registration, or to satisfy a requirement imposed under FIFRA sec. 3(c)(2)(B), must submit with the study...

  14. 40 CFR 161.34 - Flagging of studies for potential adverse effects.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Flagging of studies for potential... Provisions § 161.34 Flagging of studies for potential adverse effects. (a) Any person who submits a study of... registration, or to satisfy a requirement imposed under FIFRA sec. 3(c)(2)(B), must submit with the study...

  15. 40 CFR 161.34 - Flagging of studies for potential adverse effects.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Flagging of studies for potential... Provisions § 161.34 Flagging of studies for potential adverse effects. (a) Any person who submits a study of... registration, or to satisfy a requirement imposed under FIFRA sec. 3(c)(2)(B), must submit with the study...

  16. 14 CFR 121.621 - Alternate airport for destination: Flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Alternate airport for destination: Flag... § 121.621 Alternate airport for destination: Flag operations. (a) No person may dispatch an airplane under IFR or over-the-top unless he lists at least one alternate airport for each destination airport...

  17. 14 CFR 121.621 - Alternate airport for destination: Flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Alternate airport for destination: Flag... § 121.621 Alternate airport for destination: Flag operations. (a) No person may dispatch an airplane under IFR or over-the-top unless he lists at least one alternate airport for each destination airport...

  18. 14 CFR 121.551 - Restriction or suspension of operation: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Restriction or suspension of operation: Domestic and flag operations. 121.551 Section 121.551 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Flight Operations...

  19. Performance characteristics of blast flagging on the Cell Dyn 4000 haematology analyser.

    PubMed

    Hoedemakers, R M; Pennings, J M; Hoffmann, J J

    1999-10-01

    This study investigated the performance characteristics of blast flagging on the Abbott Cell Dyn 4000 (CD4000) haematology analyser. Our special interest was focused on the usability and prognostic value of the confidence fraction (CF), accompanying blast flagging. From our routine patient samples, 100 with blast flagging on the CD4000 were selected and examined microscopically for the presence of blasts. Blast flagging on the CD4000 resulted in a high number of false positive events. The results indicate that the blast flag confidence fraction (CF) of the CD4000 can be useful as a parameter for the prediction of the presence of blasts in samples with normal and increased white blood cell count. A model with criteria for blood smear examination following blast flagging was developed, using the blast flag CF combined with the WBC count. The model was tested and validated in a new set of 100 samples with blast flagging. Implementation of the criteria of the model resulted in a total reduction of the number of microscopic smear examinations of 45%, without losing clinically significant sensitivity.

  20. For Which It Stands: Flag Burning and the First Amendment. Teacher's Guide.

    ERIC Educational Resources Information Center

    Thompson, Marcia A.; Sass, Charles R.

    This teacher's guide is designed to accompany the two-part videotape "For Which It Stands: Flag Burning and the First Amendment." The videotape and teacher's guide should help students to: (1) understand the emotion and significance of the flag-burning issue; (2) examine the free speech aspect of the First Amendment of the Bill of…

  1. Flag Protection: A Brief History and Summary of Recent Supreme Court Decisions and Proposed Constitutional Amendment

    DTIC Science & Technology

    2005-05-19

    conduct under the First Amendment , and the symbolic importance of the United States flag. Consequently, every Congress that has convened since those...desecration under a Texas statute was inconsistent with the First Amendment and affirmed a decision of the Texas Court of Criminal Appeals that barred punishment for burning the flag as part of a public demonstration.

  2. 22 CFR 201.15 - U.S. flag vessel shipping requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false U.S. flag vessel shipping requirements. 201.15... for USAID Financing § 201.15 U.S. flag vessel shipping requirements. (a) General requirements. Unless... regarding shipping arrangements with suppliers as will assure discharge of this responsibility. (e...

  3. 22 CFR 201.15 - U.S. flag vessel shipping requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false U.S. flag vessel shipping requirements. 201.15... for USAID Financing § 201.15 U.S. flag vessel shipping requirements. (a) General requirements. Unless... regarding shipping arrangements with suppliers as will assure discharge of this responsibility. (e...

  4. 22 CFR 201.15 - U.S. flag vessel shipping requirements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false U.S. flag vessel shipping requirements. 201.15... for USAID Financing § 201.15 U.S. flag vessel shipping requirements. (a) General requirements. Unless... regarding shipping arrangements with suppliers as will assure discharge of this responsibility. (e...

  5. 22 CFR 201.15 - U.S. flag vessel shipping requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false U.S. flag vessel shipping requirements. 201.15... for USAID Financing § 201.15 U.S. flag vessel shipping requirements. (a) General requirements. Unless... regarding shipping arrangements with suppliers as will assure discharge of this responsibility. (e...

  6. 76 FR 61148 - Proposed Information Collection (Application for United States Flag for Burial Purposes) Activity...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-03

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF VETERANS AFFAIRS Proposed Information Collection (Application for United States Flag for Burial Purposes) Activity... information technology. Title: Application for United States Flag for Burial Purposes, VA Form 21-2008....

  7. 14 CFR 121.465 - Aircraft dispatcher duty time limitations: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Aircraft dispatcher duty time limitations: Domestic and flag operations. 121.465 Section 121.465 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Qualifications and Duty Time Limitations: Domestic and Flag Operations; Flight Attendant Duty Period Limitations...

  8. 14 CFR 121.465 - Aircraft dispatcher duty time limitations: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Aircraft dispatcher duty time limitations: Domestic and flag operations. 121.465 Section 121.465 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION... Qualifications and Duty Time Limitations: Domestic and Flag Operations; Flight Attendant Duty Period Limitations...

  9. FlagHouse Forum: You Say "Tomato"... and I Use a Communicator

    ERIC Educational Resources Information Center

    Exceptional Parent, 2011

    2011-01-01

    This month's "FlagHouse Forum" focuses on how to choose the communicator best-suited to a child's special need. FlagHouse--a premier global supplier of resources for special needs, education, physical activity and recreation--is pleased to partner with "Exceptional Parent" to bring its readers this informational forum. Humans communicate with each…

  10. 75 FR 82141 - Stakeholder Meetings Regarding the U.S.-Flag Great Lakes Fleet Revitalization Study

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-29

    ... making those changes. The Maritime Administration will use the study's findings to develop strategies to...-2010-0111] Stakeholder Meetings Regarding the U.S.-Flag Great Lakes Fleet Revitalization Study AGENCY... comments to inform the Maritime Administration's U.S.-Flag Great Lakes Fleet Revitalization Study. The...

  11. 14 CFR 121.663 - Responsibility for dispatch release: Domestic and flag operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Responsibility for dispatch release... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.663 Responsibility for dispatch release: Domestic and flag operations....

  12. 14 CFR 121.621 - Alternate airport for destination: Flag operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.621 Alternate airport for destination: Flag operations. (a) No person may dispatch an airplane... the dispatch release, unless— (1) The flight is scheduled for not more than 6 hours and, for at...

  13. 14 CFR 121.663 - Responsibility for dispatch release: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Responsibility for dispatch release... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.663 Responsibility for dispatch release: Domestic and flag operations....

  14. 14 CFR 121.621 - Alternate airport for destination: Flag operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.621 Alternate airport for destination: Flag operations. (a) No person may dispatch an airplane... the dispatch release, unless— (1) The flight is scheduled for not more than 6 hours and, for at...

  15. 14 CFR 121.663 - Responsibility for dispatch release: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Responsibility for dispatch release... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.663 Responsibility for dispatch release: Domestic and flag operations....

  16. 14 CFR 121.663 - Responsibility for dispatch release: Domestic and flag operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Responsibility for dispatch release... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.663 Responsibility for dispatch release: Domestic and flag operations....

  17. 14 CFR 121.621 - Alternate airport for destination: Flag operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.621 Alternate airport for destination: Flag operations. (a) No person may dispatch an airplane... the dispatch release, unless— (1) The flight is scheduled for not more than 6 hours and, for at...

  18. 14 CFR 121.663 - Responsibility for dispatch release: Domestic and flag operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Responsibility for dispatch release... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and Flight Release Rules § 121.663 Responsibility for dispatch release: Domestic and flag operations....

  19. Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads.

    PubMed

    Gerace, Erica; Moazed, Danesh

    2015-01-01

    FLAG is an affinity tag widely used for rapid and highly specific one-step protein purification. Native elution of protein from anti-FLAG antibody resins allows the identification of protein and nucleic acid binding partners and functional analysis using biochemical activity assays.

  20. 36 CFR § 504.9 - Placards, signs, banners and flags.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Placards, signs, banners and flags. § 504.9 Section § 504.9 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING SMITHSONIAN INSTITUTION BUILDINGS AND GROUNDS § 504.9 Placards, signs, banners and flags...

  1. 75 FR 66125 - Federal Land Managers' Air Quality Related Values Work Group (FLAG)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-27

    ... National Park Service Federal Land Managers' Air Quality Related Values Work Group (FLAG) AGENCY: National... revised FLAG report, and the accompanying Response to Public Comments document. The Federal Land Managers... approach for the Federal Land Managers (FLMs), i.e., National Park Service, U.S. Fish and Wildlife Service...

  2. Flag-based detection of weak gas signatures in long-wave infrared hyperspectral image sequences

    NASA Astrophysics Data System (ADS)

    Marrinan, Timothy; Beveridge, J. Ross; Draper, Bruce; Kirby, Michael; Peterson, Chris

    2016-05-01

    We present a flag manifold based method for detecting chemical plumes in long-wave infrared hyperspectral movies. The method encodes temporal and spatial information related to a hyperspectral pixel into a flag, or nested sequence of linear subspaces. The technique used to create the flags pushes information about the background clutter, ambient conditions, and potential chemical agents into the leading elements of the flags. Exploiting this temporal information allows for a detection algorithm that is sensitive to the presence of weak signals. This method is compared to existing techniques qualitatively on real data and quantitatively on synthetic data to show that the flag-based algorithm consistently performs better on data when the SINRdB is low, and beats the ACE and MF algorithms in probability of detection for low probabilities of false alarm even when the SINRdB is high.

  3. Flagging Drugs That Inhibit the Bile Salt Export Pump.

    PubMed

    Montanari, Floriane; Pinto, Marta; Khunweeraphong, Narakorn; Wlcek, Katrin; Sohail, M Imran; Noeske, Tobias; Boyer, Scott; Chiba, Peter; Stieger, Bruno; Kuchler, Karl; Ecker, Gerhard F

    2016-01-04

    The bile salt export pump (BSEP) is an ABC-transporter expressed at the canalicular membrane of hepatocytes. Its physiological role is to expel bile salts into the canaliculi from where they drain into the bile duct. Inhibition of this transporter may lead to intrahepatic cholestasis. Predictive computational models of BSEP inhibition may allow for fast identification of potentially harmful compounds in large databases. This article presents a predictive in silico model based on physicochemical descriptors that is able to flag compounds as potential BSEP inhibitors. This model was built using a training set of 670 compounds with available BSEP inhibition potencies. It successfully predicted BSEP inhibition for two independent test sets and was in a further step used for a virtual screening experiment. After in vitro testing of selected candidates, a marketed drug, bromocriptin, was identified for the first time as BSEP inhibitor. This demonstrates the usefulness of the model to identify new BSEP inhibitors and therefore potential cholestasis perpetrators.

  4. WFC3 Anomalies Flagged by the Quicklook Team

    NASA Astrophysics Data System (ADS)

    Gosmeyer, C. M.

    2017-09-01

    Like all detectors, the UVIS and IR detectors of the Wide Field Camera 3 (WFC3) on the Hubble Space Telescope are subject to detector and optical anomalies. Many of them can be corrected for or avoided with careful planning. We summarize, with examples, the various WFC3 anomalies, which when found are flagged by the WFC3 "Quicklook" team of daily image inspectors and stored in an internal database. We also give examples of known detector features and defects, and some non-standard observing modes. The aim of this report is (1) to educate users of WFC3 to more easily assess the quality of science images and (2) to serve as a reference for the WFC3 Quicklook team members in their daily visual inspections. This report was produced by C.M. Gosmeyer and The Quicklook Team.

  5. The United States Flag Stands On The Surface Of Mars

    NASA Image and Video Library

    1996-12-12

    The flag of the United States stands on the surface of Mars. It is mounted on the housing of NASA's Viking 1's nuclear power system. Also seen are the U.S. Bicentennial symbol and a student designed Viking emblem. The bright flat surface near the center is the seismometer container. This picture was taken on July 23 at about 2:30 p.m. Mars time. The view is west of the spacecraft and includes a series of low hills. The blocky hill in the center appears to be part of a crater rim. The dark, rocky stripes may be material ejected from the crater. The light areas are dune-like and may be accumulations of windblown sand or dust. http://photojournal.jpl.nasa.gov/catalog/PIA00388

  6. The United States Flag Stands On The Surface Of Mars

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The flag of the United States stands on the surface of Mars. It is mounted on the housing of Viking 1's nuclear power system. Also seen are the U.S. Bicentennial symbol and a student designed Viking emblem. The bright flat surface near the center is the seismometer container. This picture was taken on July 23 at about 2:30 p.m. Mars time. The view is west of the spacecraft and includes a series of low hills. The blocky hill in the center appears to be part of a crater rim. The dark, rocky stripes may be material ejected from the crater. The light areas are dune-like and may be accumulations of windblown sand or dust.

  7. The prevention of injuries in contact flag football.

    PubMed

    Kaplan, Yonatan; Myklebust, Grethe; Nyska, Meir; Palmanovich, Ezequiel; Victor, Jan; Witvrouw, Erik

    2014-01-01

    American flag football is a non-tackle, contact sport with many moderate to severe contact-type injuries reported. A previous prospective injury surveillance study by the authors revealed a high incidence of injuries to the fingers, face, knee, shoulder and ankle. The objectives of the study were to conduct a pilot-prospective injury prevention study in an attempt to significantly reduce the incidence and the severity of injuries as compared to a historical cohort, as well as to provide recommendations for a future prospective injury prevention study. A prospective injury prevention study was conducted involving 724 amateur male (mean age: 20.0 ± 3.1 years) and 114 female (mean age: 21.2 ± 7.2 years) players. Four prevention measures were implemented: the no-pocket rule, self-fitting mouth guards, ankle braces (for those players with recurrent ankle sprains) and an injury treatment information brochure. An injury surveillance questionnaire was administered to record all time-loss injuries sustained in game sessions. There was a statistically significant reduction in the number of injured players, the number of finger/hand injuries, the incidence rate and the incidence proportion between the two cohorts (p < 0.05). This one-season pilot prevention study has provided preliminary evidence that finger/hand injuries can be significantly reduced in flag football. Prevention strategies for a longer, prospective, randomised-controlled injury prevention study should include the strict enforcement of the no-pocket rule, appropriate head gear, the use of comfortable-fitting ankle braces and mouth guards, and changing the blocking rules of the game.

  8. Red flags to screen for malignancy and fracture in patients with low back pain: systematic review

    PubMed Central

    Williams, Christopher M; Henschke, Nicholas; Hancock, Mark J; Ostelo, Raymond W J G; de Vet, Henrica C W; Macaskill, Petra; Irwig, Les; van Tulder, Maurits W; Koes, Bart W; Maher, Christopher G

    2013-01-01

    Objective To review the evidence on diagnostic accuracy of red flag signs and symptoms to screen for fracture or malignancy in patients presenting with low back pain to primary, secondary, or tertiary care. Design Systematic review. Data sources Medline, OldMedline, Embase, and CINAHL from earliest available up to 1 October 2013. Inclusion criteria Primary diagnostic studies comparing red flags for fracture or malignancy to an acceptable reference standard, published in any language. Review methods Assessment of study quality and extraction of data was conducted by three independent assessors. Diagnostic accuracy statistics and post-test probabilities were generated for each red flag. Results We included 14 studies (eight from primary care, two from secondary care, four from tertiary care) evaluating 53 red flags; only five studies evaluated combinations of red flags. Pooling of data was not possible because of index test heterogeneity. Many red flags in current guidelines provide virtually no change in probability of fracture or malignancy or have untested diagnostic accuracy. The red flags with the highest post-test probability for detection of fracture were older age (9%, 95% confidence interval 3% to 25%), prolonged use of corticosteroid drugs (33%, 10% to 67%), severe trauma (11%, 8% to 16%), and presence of a contusion or abrasion (62%, 49% to 74%). Probability of spinal fracture was higher when multiple red flags were present (90%, 34% to 99%). The red flag with the highest post-test probability for detection of spinal malignancy was history of malignancy (33%, 22% to 46%). Conclusions While several red flags are endorsed in guidelines to screen for fracture or malignancy, only a small subset of these have evidence that they are indeed informative. These findings suggest a need for revision of many current guidelines. PMID:24335669

  9. CO2 flagging - an improved method for the collection of questing ticks

    PubMed Central

    2012-01-01

    Background Most epidemiological studies on tick-borne pathogens involve collection of ticks from the environment. An efficient collection method is essential for large sample pools. Our main aim was to evaluate the efficacy of a new method, where traditional flagging was enhanced by the use of CO2 dispersed into the white flannel. The CO2 was spread through a rubber hose network inserted into the flag blanket. The research was conducted in spring, in March-April 2011 in two locations from Cluj County, Romania. Methods The research was conducted in March-April 2011 in two locations from Cluj County, Romania. The flag to be tested contained a fine silicone rubber hose network which dispersed the CO2 in the shaft. On each collection site n=30 samplings were performed. Each sampling consisted in the simultaneous use of both flags (with and without CO2) by two persons. The CO2 concentration level on the flag canvas surface was measured. The efficacy of the method was determined by counting comparatively the total number of ticks and separate developmental stage count. Results Using the CO2 improved flag, 2411 (59%) Ixodes ricinus and 100 (53.8%) Dermacentor marginatus ticks were captured, while the CO2-free flag accounted for the collection of 1670 I. ricinus (41%) and 86 (46.2%) D. marginatus ticks. The addition of CO2 prompted a concentration difference on the surface of the flag ranging between 756.5 and 1135.0 ppm with a mean value of 848.9 ppm. Conclusion The study showed that the CO2 enhanced sweep flag increased the ability of I. ricinus (p < 0001) but not of D. marginatus to be attracted to the flag blanket. PMID:22720872

  10. Experimental investigation of the elastic flag spontaneous flapping in water flow

    NASA Astrophysics Data System (ADS)

    Jia, Yongxia; Jia, Lichao; Su, Zhuang; Zhu, Yiding; Yuan, Huijing; Lee, Cunbiao

    2015-11-01

    The flapping stability and the response of a thin two-dimensional flag of low bending rigidity to the Reynolds number was investigated. The three relevant non-dimensional parameters governing fluid-structure problems that concern the interaction of elastic flags with high-speed fluid flows are the structure-to-fluid mass ratio, the non-dimensional bending rigidity and the Reynolds number. To study the mechanisms of the transition from the periodic flapping to chaotic flapping, we use PIV and flow visualization techniques to obtain the whole flow field around the midspan of the immersed elastic flag interacting with fluid in both periodic and chaotic states. A moving interface detection technique is used to determine the flag position and velocity. Virtual particle images are imposed in the flag region in the PIV algorithm, of which the displacements are evaluated by the flag movement. We find that the value of St is constrained in the narrow range of 0.2 < St < 0.31 based on the flapping amplitude.We find that the transition to chaos occurs at a critical Reynolds number Re = 60800. For the larger Reynolds number, the high-strength vortices are distributed in a detached region away from the free end of the flag during the intermittent snapping events in the chaotic regime.

  11. Red flag screening for low back pain: nothing to see here, move along: a narrative review.

    PubMed

    Cook, Chad E; George, Steven Z; Reiman, Michael P

    2017-09-18

    Screening for red flags in individuals with low back pain (LBP) has been a historical hallmark of musculoskeletal management. Red flag screening is endorsed by most LBP clinical practice guidelines, despite a lack of support for their diagnostic capacity. We share four major reasons why red flag screening is not consistent with best practice in LBP management: (1) clinicians do not actually screen for red flags, they manage the findings; (2) red flag symptomology negates the utility of clinical findings; (3) the tests lack the negative likelihood ratio to serve as a screen; and (4) clinical practice guidelines do not include specific processes that aid decision-making. Based on these findings, we propose that clinicians consider: (1) the importance of watchful waiting; (2) the value-based care does not support clinical examination driven by red flag symptoms; and (3) the recognition that red flag symptoms may have a stronger relationship with prognosis than diagnosis. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  12. Development and Application of a Visible-Infrared Rain Flag for Scatterometer Data.

    NASA Astrophysics Data System (ADS)

    Grassotti, Christopher; Leidner, S. Mark; Louis, Jean-François; Hoffman, Ross N.

    1999-06-01

    The authors report on characteristics of a rain flag derived from collocation of visible and infrared image data with rain rates over the North Atlantic Ocean obtained from microwave imagery (SSM/I) during a 3-week period (15 October 1996-2 November 1996). The rain flag has been developed as part of an effort to provide an indication of contamination by heavy rainfall in NASA scatterometer datasets. The primary results of this analysis indicate 1) that a simple albedo/infrared brightness temperature threshold is capable of flagging most of the heavy rainfall, though with a fairly high rate of false alarms, and 2) that the small difference in optimal threshold between the Tropics and midlatitudes can probably be ignored. Use of the rain flag in 12 assimilation experiments during this period showed that the number of rain-flagged wind vector cells is generally less than 1% of the number of cells. Overall, the impact from using the rain-flagged data is generally less than 5 m s1 and localized (less than 5° of latitude and longitude). However, in some cases, the effect of excluding just one to five rain-flagged points can change the resulting analysis significantly, because their placement is critical for defining the flow along a front or some other shear-dominated environment.

  13. Performance evaluation of the Sysmex XN-1000 hematology analyzer in assessment of the white blood cell count differential in pediatric specimens.

    PubMed

    Becker, P-H; Fenneteau, O; Da Costa, L

    2016-02-01

    The automated XN-1000 hematology analyzer enables to perform a blood cell count and a leukocyte differential. When abnormal cells were detected, a flag was generated by the analyzer and a manual microscopic examination of the corresponding blood film was performed. We compared the white blood cell differentials provided by the automated hematology analyzer XN-1000 in a pediatric population (n = 765) with those obtained through microscopic examination by cytologists and those obtained using a previous version of this analyzer, the XE-2100. Leukocytes count as well as flags sensitivity and specificity was analyzed. The leukocytes count provided by the analyzer is in good accordance with the differential obtained by manual count in children older than 3 months. The sensitivity for blast detection is 99% and the detection of reactive cells is 63%. The flag specificity remains low (<35%) for blood samples collected from infants between 8 days and 2 years of age, but increases up to 67% thereafter. The results obtained with the XN-1000 analyzer show an improvement in comparison with those obtained with the XE-2100 analyzer. The automated WBC differential provided by the XN-1000 analyzer in the pediatric setting is accurate, but a meticulous microscopic examination of blood smears remains necessary for infants up to 3 months of age to validate the analyzer flags. © 2015 John Wiley & Sons Ltd.

  14. Particle Property Data Quality Flags for the MISR Aerosol Product

    NASA Astrophysics Data System (ADS)

    Gaitley, B. J.; Kahn, R. A.; Garay, M. J.

    2013-12-01

    The MISR instrument aboard the NASA Earth Observing System's Terra satellite has the unique capability to retrieve aerosol properties under favorable conditions. General aerosol type retrieval quality guidelines are provided in the MISR Data Quality Statement and related publications. The retrieved value of aerosol type is more sensitive to scene conditions than aerosol optical depth, and more difficult to validate, as there is very little coincident aerosol type validation data. Here we report on the steps we are taking to provide an aerosol-type data quality flag, to be provided with each individual retrieval result. Due to the lack of validation data for comparison, our main approach is to evaluate the self-consistency of aerosol type retrieval values for regions where particular aerosol types are known to dominate. Some factors affecting aerosol type retrieval quality that can be assessed pre-retrieval are the number of MISR cameras available, the range of scattering angles viewed, and surface conditions such as shallow water or seasonal coastal runoff. Factors that must be assessed post-retrieval include values of retrieved aerosol optical depth and the number and type of mixtures successfully passing the MISR algorithm acceptance criteria. Regional monthly plots with MISR measurements binned at 0.5 degree resolution and color-coded stratification of one or more parameters are the main tools for identifying locations and times where different aerosol types are retrieved. The statistics of individual MISR values such as mid-visible AOD, number and type of mixtures passing, number of cameras used, the range and maximum scattering angles, are studied as joint distributions on a region-by-region basis. From these, a synthesis of the self-consistency and agreement with expectation is made, effectively indicating the quality of the aerosol type constrains to the extent possible, and thresholds for assigning quality flags are assessed. Multiple-month summaries

  15. An Auto-flag Method of Radio Visibility Data Based on Support Vector Machine

    NASA Astrophysics Data System (ADS)

    Hui-mei, Dai; Ying, Mei; Wei, Wang; Hui, Deng; Feng, Wang

    2017-01-01

    The Mingantu Ultrawide Spectral Radioheliograph (MUSER) has entered a test observation stage. After the construction of the data acquisition and storage system, it is urgent to automatically flag and eliminate the abnormal visibility data so as to improve the imaging quality. In this paper, according to the observational records, we create a credible visibility set, and further obtain the corresponding flag model of visibility data by using the support vector machine (SVM) technique. The results show that the SVM is a robust approach to flag the MUSER visibility data, and can attain an accuracy of about 86%. Meanwhile, this method will not be affected by solar activities, such as flare eruptions.

  16. Subliminal exposure to national flags affects political thought and behavior

    PubMed Central

    Hassin, Ran R.; Ferguson, Melissa J.; Shidlovski, Daniella; Gross, Tamar

    2007-01-01

    Political thought and behavior play an important role in our lives, from ethnic tensions in Europe, to the war in Iraq and the Middle Eastern conflict, to parliamentary and presidential elections. However, little is known about how the individual's political attitudes and decisions are shaped by subtle national cues that are so prevalent in our environment. We report a series of experiments that show that subliminal exposure to one's national flag influences political attitudes, intentions, and decisions, both in laboratory settings and in “real-life” behavior. Furthermore, this manipulation consistently narrowed the gap between those who score high vs. low on a scale of identification with Israeli nationalism. The first two experiments examined participants' stance toward the Israeli–Palestinian conflict and the Jewish settlers in the West Bank. Experiment 3 examined voting intentions and actual voting in Israel's recently held general elections. The results portray a consistent picture: subtle reminders of one's nationality significantly influence political thought and overt political behavior. PMID:18056813

  17. Apollo 17 Astronaut and United States Flag on Lunar Surface

    NASA Technical Reports Server (NTRS)

    1972-01-01

    This is an Apollo 17 Astronaut standing upon the lunar surface with the United States flag in the background. The seventh and last manned lunar landing and return to Earth mission, the Apollo 17, carrying a crew of three astronauts: Mission Commander Eugene A. Cernan; Lunar Module pilot Harrison H. Schmitt; and Command Module pilot Ronald E. Evans lifted off on December 7, 1972 from the Kennedy Space Flight Center (KSC). Scientific objectives of the Apollo 17 mission included geological surveying and sampling of materials and surface features in a preselected area of the Taurus-Littrow region, deploying and activating surface experiments, and conducting in-flight experiments and photographic tasks during lunar orbit and transearth coast (TEC). These objectives included: Deployed experiments such as the Apollo lunar surface experiment package (ALSEP) with a Heat Flow experiment, Lunar seismic profiling (LSP), Lunar surface gravimeter (LSG), Lunar atmospheric composition experiment (LACE)and Lunar ejecta and meteorites (LEAM). The mission also included Lunar Sampling and Lunar orbital experiments. Biomedical experiments included the Biostack II Experiment and the BIOCORE experiment. The mission marked the longest Apollo mission, 504 hours, and the longest lunar surface stay time, 75 hours, which allowed the astronauts to conduct an extensive geological investigation. They collected 257 pounds (117 kilograms) of lunar samples with the use of the Marshall Space Flight Center designed Lunar Roving Vehicle (LRV). The mission ended on December 19, 1972

  18. U.S. BICENTENNIAL EXPOSITION & FINISHED PAINTED AMERICAN FLAG ON VAB

    NASA Technical Reports Server (NTRS)

    1976-01-01

    Finishing touches on the largest American flag ever painted were completed today on the south side of the Vehicle Assembly Building at KSC's Launch Complex 39. The flag was painted as part of 3rd Century America, the U. S. Bicentennial Exposition on Science and Technology to be held here from May 30 through September 7. The flag is located on the western side of the VAB's south wall. Still to be painted is the American Revolution Bicentennial Administration's [ARBA] distinctive emblem. This will be placed on the eastern side of the south wall and should be completed by mid-May. The flag is 209 feet long and 100 feet in width. Each of its 13 red and white bars is nearly 8 and a half feet wide. The ARBA symbol will be 110 feet in diameter.

  19. 38. FLAG/ADMIRAL BRIDGE PORT LOOKING TO STARBOARD SHOWING RADAR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    38. FLAG/ADMIRAL BRIDGE - PORT LOOKING TO STARBOARD SHOWING RADAR SCOPE, ADMIRAL'S CHAIR, GYRO REPEATER AND VARIOUS COMMUNICATION SYSTEMS. - U.S.S. HORNET, Puget Sound Naval Shipyard, Sinclair Inlet, Bremerton, Kitsap County, WA

  20. A single exposure to the American flag shifts support toward Republicanism up to 8 months later.

    PubMed

    Carter, Travis J; Ferguson, Melissa J; Hassin, Ran R

    2011-08-01

    There is scant evidence that incidental cues in the environment significantly alter people's political judgments and behavior in a durable way. We report that a brief exposure to the American flag led to a shift toward Republican beliefs, attitudes, and voting behavior among both Republican and Democratic participants, despite their overwhelming belief that exposure to the flag would not influence their behavior. In Experiment 1, which was conducted online during the 2008 U.S. presidential election, a single exposure to an American flag resulted in a significant increase in participants' Republican voting intentions, voting behavior, political beliefs, and implicit and explicit attitudes, with some effects lasting 8 months after the exposure to the prime. In Experiment 2, we replicated the findings more than a year into the current Democratic presidential term. These results constitute the first evidence that nonconscious priming effects from exposure to a national flag can bias the citizenry toward one political party and can have considerable durability.

  1. FLAG Simulations of the Elasticity Test Problem of Gavrilyuk et al.

    SciTech Connect

    Kamm, James R.; Runnels, Scott R.; Canfield, Thomas R.; Carney, Theodore C.

    2014-04-23

    This report contains a description of the impact problem used to compare hypoelastic and hyperelastic material models, as described by Gavrilyuk, Favrie & Saurel. That description is used to set up hypoelastic simulations in the FLAG hydrocode.

  2. Artist's rendering of astronaut Neil Armstrong planting U.S. flag on Moon

    NASA Technical Reports Server (NTRS)

    1969-01-01

    Artist's Concept: Apollo 11 astronaut Neil Armstrong, after stepping onto the lunar surface, will plant the United States flag in its soil. The flag will be made of nylone, size 3- by 5 feet on a staff 8 feet long. During flight it will be stowed in two 4-foot sections strapped to the Lunar Module ladder. Armstrong's first assignment after stepping off the ladder is to pull a 'D' ring to start a television camera. The second assignment is to erect the U.S. flag. The flag will appear to be flying in a breeze. This is done with a spring-loaded wire in the nylon cloth. With everything is working normally, this will be observed on live television.

  3. Navigating a path delineated by colored flags: an approach for a 2011 IGVC requirement

    NASA Astrophysics Data System (ADS)

    Szmatula, Alex; Parrish, Matt; Krishnan, Mohan; Paulik, Mark; Mohammad, Utayba; Luo, Chaomin

    2012-01-01

    In this work, we address a situation presented as a new requirement for the Autonomous Challenge portion of the 2011 Intelligent Ground Vehicle Competition (IGVC). This new requirement is to navigate between red and green colored flags placed within the normal white painted lane lines. The regular vision algorithms had to be enhanced to reliably identify and localize the colored flags, while the Navigation algorithms had to be modified to satisfy the constraints placed on the robot while transiting through the flag region. The challenge in finding a solution was the size of the flags, the possibility of loosing them against the background, as well as their movement in the wind. The attendant possibility of false positives and negatives also needed to be addressed to increase reliability of detection. Preliminary tests on the robot have produced positive results.

  4. Artist's rendering of astronaut Neil Armstrong planting U.S. flag on Moon

    NASA Technical Reports Server (NTRS)

    1969-01-01

    Artist's Concept: Apollo 11 astronaut Neil Armstrong, after stepping onto the lunar surface, will plant the United States flag in its soil. The flag will be made of nylone, size 3- by 5 feet on a staff 8 feet long. During flight it will be stowed in two 4-foot sections strapped to the Lunar Module ladder. Armstrong's first assignment after stepping off the ladder is to pull a 'D' ring to start a television camera. The second assignment is to erect the U.S. flag. The flag will appear to be flying in a breeze. This is done with a spring-loaded wire in the nylon cloth. With everything is working normally, this will be observed on live television.

  5. Validation of the Sysmex CS5100 coagulation analyzer and comparison to the Stago STA-R analyzer for routine coagulation parameters.

    PubMed

    Geens, T; Vertessen, F; Malfait, R; Deiteren, K; Maes, M B

    2015-06-01

    The CS5100 analyzer (Sysmex) was validated for the determination of routine coagulation parameters. This fully automated coagulation analyzer uses multiple wavelength technology to perform coagulation (e.g., activated partial thromboplastin time - APTT, prothrombin time - PT, fibrinogen - FBG), chromogenic (e.g., antithrombin - AT) and immunological (e.g., D-dimers - DDi) assays. A comparison with the currently used STA-R Evolution (Stago) was performed. Validation and verification of reference values of the CS5100 was performed in accordance to CLSI guidelines (H57-A, H47-A2, and C28-A3). As a different detection system and reagents were used, significant differences were observed (e.g. APTT). The within-day and between-day imprecision, accuracy and total error were all acceptable. The reference values defined by the manufacturer could be used except for APTT. In our settings, the therapeutic anti-Xa range of 0.3-0.7 IU/mL corresponded to an APTT range of 60-100 s (Dade actin FS reagent). The APTT reagent showed factor sensitivities between 46 and 72% for FVIII, IX, XI and XII while the PT reagent showed sensitivities between 34 and 52% for FII, FV, FXII, and FX. In conclusion, the CS5100 instrument is suitable for the determination of the APTT, PT, FBG, DDi and AT in routine analysis. © 2014 John Wiley & Sons Ltd.

  6. Investigation of morphological changes for the discrimination of nucleated red blood cells and other leukocytes in Sysmex XN hematology analyzer scattergrams using transmission electron microscopy.

    PubMed

    Kaido, Masako; Takagi, Yuri; Kono, Mari; Nakazawa, Fumie; Yamamoto, Shiori; Wada, Atsushi; Morikawa, Takashi

    2017-08-01

    The WNR channel of the XN-Series automated hematology analyzer (Sysmex) counts white blood cells (WBCs) and simultaneously performs a differential counting of basophils and nucleated red blood cells (NRBCs). The detection process involves exposing the cells to WNR-specific reagents containing an acidic detergent and a fluorescent dye and measuring the intensity of the forward scattered light (FSC) and side fluorescence light (SFL). We treated isolated peripheral WBCs and NRBCs with specific reagents and assessed the morphological changes in NRBCs and each leukocyte type using transmission electron microscopy (TEM). The results from a flow cytometer (FCM) showed that, after exposure to the reagents, basophils appeared on the highest FSC and SFL areas compared to other leukocytes on the WNR scattergram. Owing to the hemolysis of reticulocytes and erythrocytes, NRBCs that survived the reagent treatment could be distinguished by their lower intensity than those of the other leukocytes on the WNR scattergram. We investigated the significance of the relationship between the TEM and FCM results after the reagent treatment. We confirmed that the WNR channel differentiates the blood cells on the WNR scattergram based on differences in the amount of residual cytoplasm and nucleic acids.

  7. On Finsler surfaces of constant flag curvature with a Killing field

    NASA Astrophysics Data System (ADS)

    Bryant, R. L.; Huang, L.; Mo, X.

    2017-06-01

    We study two-dimensional Finsler metrics of constant flag curvature and show that such Finsler metrics that admit a Killing field can be written in a normal form that depends on two arbitrary functions of one variable. Furthermore, we find an approach to calculate these functions for spherically symmetric Finsler surfaces of constant flag curvature. In particular, we obtain the normal form of the Funk metric on the unit disk D2.

  8. Mitigating Task Saturation in Critical Care Air Transport Team Red Flag Checklist

    DTIC Science & Technology

    2015-04-14

    with a 35 being the highest (and therefore the best) score attainable. There was no statistical difference in the average red flag score for the...AFRL-SA-WP-SR-2015-0008 Mitigating Task Saturation in Critical Care Air Transport Team Red Flag Checklist Dr. Bradley...REPORT DOCUMENTATION PAGE Form Approved OMB No . 0704-0188 Public reporting burden for this collection of information is estimated to average 1 hour per

  9. Yellow flag scores in a compensable New Zealand cohort suffering acute low back pain

    PubMed Central

    Grimmer-Somers, Karen; Prior, Mathew; Robertson, Jim

    2008-01-01

    Background Despite its high prevalence, most acute low back pain (ALBP) is nonspecific, self-limiting with no definable pathology. Recurrence is prevalent, as is resultant chronicity. Psychosocial factors (yellow flags comprising depression and anxiety, negative pain beliefs, job dissatisfaction) are associated with the development of chronic LBP. Methods A national insurer (Accident Compensation Corporation, New Zealand [NZ]), in conjunction with a NZ primary health organization, piloted a strategy for more effective management of patients with ALBP, by following the NZ ALBP Guideline. The guidelines recommend the use of a psychosocial screening instrument (Yellow Flags Screening Instrument, a derivative of Örebro Musculoskeletal Pain Questionnaire). This instrument was recommended for administration on the second visit to a general medical practitioner (GP). This paper tests whether published cut-points of yellow flag scores to predict LBP claims length and costs were valid in this cohort. Results Data was available for 902 claimants appropriately enrolled into the pilot. 25% claimants consulted the GP once only, and thus were not requested to provide a yellow flag score. Yellow flag scores were provided by 48% claimants who consumed two or more GP services. Approximately 60% LBP presentations resolved within five GP visits. Yellow flag scores were significantly and positively associated with treatment costs and service use, although the association was nonlinear. Claimants with moderate yellow flag scores were similarly likely to incur lengthy claims as claimants with at-risk scores. Discussion Capturing data on psychosocial factors for compensable patients with ALBP has merit in predicting lengthy claims. The validity of the published yellow flag cut-points requires further testing. PMID:21197284

  10. 46 CFR 381.9 - Available U.S.-flag service.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... at U.S. Great Lakes ports, a joint service involving a foreign-flag vessel(s) carrying cargo no farther than a Canadian port(s) or other point(s) on the Gulf of St. Lawrence, with transshipment via a U... point on the Gulf of St. Lawrence and a U.S.-flag leg for the remainder of the voyage. The “fair...

  11. 46 CFR 381.9 - Available U.S.-flag service.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... at U.S. Great Lakes ports, a joint service involving a foreign-flag vessel(s) carrying cargo no farther than a Canadian port(s) or other point(s) on the Gulf of St. Lawrence, with transshipment via a U... point on the Gulf of St. Lawrence and a U.S.-flag leg for the remainder of the voyage. The “fair...

  12. Flags at half-staff in memorial of STS 51-L crewmembers

    NASA Image and Video Library

    1986-02-03

    S86-26436 (31 Jan. 1986) --- The United States flag, in front of the Johnson Space Center’s (JSC) project management building, flies at half-mast in memory of the seven space shuttle Challenger crew members who lost their lives on Jan. 28, 1986. Earlier today, memorial services about 100 yards from the flag drew thousands of JSC employees, friends and family of the STS-51L astronauts and payload specialists. Photo credit: NASA

  13. Freestanding Flag-Type Triboelectric Nanogenerator for Harvesting High-Altitude Wind Energy from Arbitrary Directions.

    PubMed

    Zhao, Zhenfu; Pu, Xiong; Du, Chunhua; Li, Linxuan; Jiang, Chunyan; Hu, Weiguo; Wang, Zhong Lin

    2016-02-23

    Wind energy at a high altitude is far more stable and stronger than that near the ground, but it is out of reach of the wind turbine. Herein, we develop an innovative freestanding woven triboelectric nanogenerator flag (WTENG-flag) that can harvest high-altitude wind energy from arbitrary directions. The wind-driven fluttering of the woven unit leads to the current generation by a coupled effect of contact electrification and electrostatic induction. Systematic study is conducted to optimize the structure/material parameters of the WTENG-flag to improve the power output. This 2D WTENG-flag can also be stacked in parallel connections in many layers for a linearly increased output. Finally, a self-powered high-altitude platform with temperature/humidity sensing/telecommunicating capability is demonstrated with the WTENG-flag as a power source. Due to the light weight, low cost, and easy scale-up, this WTENG-flag has great potential for applications in weather/environmental sensing/monitoring systems.

  14. Psychometric analysis of the Systematic Observation of Red Flags for autism spectrum disorder in toddlers.

    PubMed

    Dow, Deanna; Guthrie, Whitney; Stronach, Sheri T; Wetherby, Amy M

    2017-04-01

    The purpose of this study was to examine the utility of the Systematic Observation of Red Flags as an observational level-two screening measure to detect risk for autism spectrum disorder in toddlers when used with a video-recorded administration of the Communication and Symbolic Behavior Scales. Psychometric properties of the Systematic Observation of Red Flags were examined in a sample of 247 toddlers of 16- to 24 months old: 130 with autism spectrum disorder, 61 with developmental delays, and 56 typically developing. Individual items were examined for performance to create an algorithm with improved sensitivity and specificity, yielding a total Composite score and Domain scores for Social Communication and Restricted Repetitive Behaviors. Codes indicating clear symptom presence were collapsed to yield a count of the number of Red Flags for the overall scale and each symptom domain. Results indicated significant group differences with large effects for the Composite, both Domain scores, and Red Flags score, and good discrimination (area under the curve = 0.84-0.87) between autism spectrum disorder and nonspectrum groups for the Composite, Social Communication Domain, and Social Communication Red Flags score. The Systematic Observation of Red Flags provides an observational screening measure for 16- to 24-month-olds with good discrimination, sensitivity, and specificity. A cutoff of 20 on the Composite is recommended to optimally detect autism spectrum disorder risk.

  15. Flexible Tricolor Flag-liked Microribbons Array with Enhanced Conductive Anisotropy and Multifunctionality

    PubMed Central

    Ma, Qianli; Yu, Wensheng; Dong, Xiangting; Yang, Ming; Wang, Jinxian; Liu, Guixia

    2015-01-01

    Anisotropically conductive materials are important components in subminiature devices. However, at this stage, some defects have limited practical applications of them, especially low anisotropic degree and high cost. Here, we report novel tricolor flag-liked microribbons array prepared by electrospinning technique. The tricolor flag-liked microribbons array is composed of parallel microribbons, and each microribbon consists of three different regions, just like tricolor flag. The tricolor flag-liked microribbons array is only electrically conductive in the direction parallel to the microribbons, whereas in the perpendicular and thickness directions are insulative. The electrical conductivity along parallel direction reaches up to 8 orders of magnitude higher than that along perpendicular direction. The degree of anisotropy in present study is increased by 2 orders of magnitude than that of the anisotropically conductive material in references reported before. Besides, other functions can be conveniently assembled into tricolor flag-liked microribbons array to realize multifunctionality. Owing to the high electrical anisotropy and multifunctionality, tricolor flag-liked microribbons array will have important applications. Furthermore, a universal technique to prepare microribbons with three functional regions has been established for fabricating excellent multifunctional materials. PMID:26412500

  16. Injuries can be prevented in contact flag football!

    PubMed

    Kaplan, Yonatan; Myklebust, Grethe; Nyska, Meir; Palmanovich, Ezequiel; Victor, J; Witvrouw, E

    2016-06-01

    This original prospective cohort study was conducted in an attempt to significantly reduce the incidence and the severity of injuries in an intervention cohort as compared to a two-season historical cohort, and to provide recommendations to the International Federation of Football (IFAF) pertaining to prevention measures to make the game safer. A total of 1,260 amateur male (mean age: 20.4 ± 3.9 years) and 244 female (mean age: 18.5 ± 1.7 years) players participated in the study. Four prevention measures were implemented: the no-pocket rule, self-fitting mouth guards, ankle braces (for those players with recurrent ankle sprains) and an injury treatment information brochure. All time-loss injuries sustained in game sessions were recorded by the off-the-field medical personnel and followed up by a more detailed phone injury surveillance questionnaire. There was a 54 % reduction in the total number of injuries and a significant reduction in the incidence rate and incidence proportion between the intervention cohorts as compared to the historical cohort (p < 0.001). There was no statistically significant reduction in the number of injuries in any of the body parts, except for in hand/wrist injuries related to the use of pockets (p < 0.001), as well as the severity of mild-moderate injuries (p < 0.05). This study provided evidence that hand/wrist injuries can be significantly reduced in flag football. Recommendations to the IFAF include strict enforcement of the no-pocket rule, the use of soft headgear, comfortable-fitting ankle braces and mouth guards and additionally, to change game rules concerning blocking. II.

  17. Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses: Part I--precision, linearity, and accuracy of complete blood cell count.

    PubMed

    Bauer, Natali; Nakagawa, Julia; Dunker, Cathrin; Failing, Klaus; Moritz, Andreas

    2011-11-01

    The automated laser-based hematology analyzer Sysmex XT-2000iV™ providing a complete blood cell count (CBC) and 5-part differential has been introduced in large veterinary laboratories. The aim of the current study was to determine precision, linearity, and accuracy of the Sysmex analyzer. Reference method for the accuracy study was the laser-based hematology analyzer ADVIA® 2120. For evaluation of accuracy, consecutive fresh blood samples from healthy and diseased cats (n = 216), dogs (n = 314), and horses (n = 174) were included. A low intra-assay coefficient of variation (CV) of approximately 1% was seen for the CBC except platelet count (PLT). An intra-assay CV ranging between 2% and 5.5% was evident for the differential count except for feline and equine monocytes (7.7%) and horse eosinophils (15.7%). Linearity was excellent for white blood cell count (WBC), hematocrit value, red blood cell count (RBC), and PLT. For all evaluated species, agreement was excellent for WBC and RBC, with Spearman rank correlation coefficients (r(s)) ranging from >0.99 to 0.98. Hematocrit value correlated excellently in cats and dogs, whereas for horses, a good correlation was evident. A good correlation between both analyzers was seen in feline and equine PLT (r(s) = 0.89 and 0.92, respectively), whereas correlation was excellent for dogs (r(s) = 0.93). Biases were close to 0 except for mean corpuscular hemoglobin concentration (4.11 to -7.25 mmol/l) and canine PLT (57 × 10(9)/l). Overall, the performance of the Sysmex analyzer was excellent and compared favorably with the ADVIA analyzer.

  18. Special Measurement Clothing and Footwear, Orthopedic Footwear, Guidons, Streamers, and Flags (Supplementation is Permitted at all Levels)

    DTIC Science & Technology

    2007-11-02

    GUIDONS , STREAMERS, AND FLAGS (Supplementation is permitted at all levels.) Headquarters Departments of the Army, Defense Logistic AgencyDepartment of...Special Measurement Clothing and Footwear, Orthopedic Footwear, Guidons , Streamers, and Flags Unclassified 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c...NAVSUPINST 4400.70C/MCO 4400.137A SPECIAL MEASUREMENT CLOTHING AND FOOTWEAR, ORTHOPEDIC FOOTWEAR, GUIDONS , STREAMERS, AND FLAGS (Supplementation is

  19. Changes in haematology measurements with the Sysmex XT-2000iV during storage of feline blood sampled in EDTA or EDTA plus CTAD.

    PubMed

    Granat, Fanny; Geffré, Anne; Bourgès-Abella, Nathalie; Braun, Jean-Pierre; Trumel, Catherine

    2013-06-01

    In veterinary medicine a complete blood cell count (CBC) cannot always be performed within 24 h as usually recommended, particularly for specimens shipped to a reference laboratory. This raises the question of the stability of the variables, especially in ethylenediamine tetra-acetic acid (EDTA) feline blood specimens, known to be prone to in vitro platelet aggregation. Citrate, theophylline, adenosine and dipyridamole (CTAD) has been reported to limit platelet aggregation in feline blood specimens. The aim of this study was to measure the stability of the haematological variables and the platelet aggregation score in EDTA and EDTA plus CTAD (EDCT) feline blood specimens during 48 h of storage at room temperature. Forty-six feline EDTA and EDCT blood specimens were analysed with a Sysmex XT-2000iV analyser, and the platelet count and score of platelet aggregation were estimated immediately and after 24 and 48 h of storage. A significant increase in mean corpuscular volume, haematocrit, reticulocyte and eosinophil counts, and a significant decrease in mean corpuscular haemoglobin concentration and monocyte count were observed. Haemoglobin, mean corpuscular haemoglobin, and red blood cell, white blood cell, neutrophil and lymphocyte counts remained stable. Changes in reticulocyte indexes with time (low fluorescence ratio, medium fluorescence ratio, high fluorescence ratio and immature reticulocyte fraction) were not significant. Changes were generally more pronounced in EDTA than in EDCT. Platelet aggregation decreased markedly in initially highly aggregated EDTA specimens, and increased slightly in initially non- or mildly-aggregated EDTA or EDCT specimens. Platelet counts increased and decreased, or remained stable, respectively. CTAD can reduce storage-induced changes of the haematological variables in feline samples, thus improving the reliability of a CBC and limiting clinical misinterpretations.

  20. Evaluation of Sysmex XN-1000 High-Sensitive Analysis (hsA) Research Mode for Counting and Differentiating Cells in Cerebrospinal Fluid.

    PubMed

    Fleming, Chérina; Russcher, Henk; Brouwer, Rob; Lindemans, Jan; de Jonge, Robert

    2016-03-01

    Counting cells in cerebrospinal fluid (CSF) using automated analyzers is generally problematic due to low precision at low cell numbers. To overcome this limitation, Sysmex (Kobe, Japan) developed the high-sensitive analysis (hsA) research mode specifically for counting cells in fluids that contain low cell counts. We evaluated this mode by counting RBCs, WBCs, and differentiated WBCs in CSF samples. We analyzed 248 CSF samples using the hsA mode and compared these results with those obtained using the manual counting method. We also evaluated the linearity, detection limits, carryover, and precision of the hsA mode. Using the hsA mode, the lower limit of quantification for RBCs and WBCs was 10 and 2 cells/μL, respectively. Comparing the two methods revealed good agreement with respect to WBCs (y = 1.08x + 0.52), RBCs (y = 1.07x + 0.00), lymphocytes (y = 1.00x + 0.00), neutrophils (y = 1.05x + 0.00), and monocytes (y = 0.88x + 0.07). Regression analysis for samples containing low WBCs (<10 cells/μL) and low RBCs (<50 cells/μL) also had good agreement, although a slight positive bias was found for RBCs. Linearity was good (r(2) ≥ 0.99) for all parameters evaluated. Carryover was negligible and never exceeded 0.04%. The XN hsA research mode provides reliable cell counts in CSF samples, even in samples containing low numbers of WBCs and RBCs. © American Society for Clinical Pathology, 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  1. Validation of the body fluid module on the new Sysmex XN-1000 for counting blood cells in cerebrospinal fluid and other body fluids.

    PubMed

    Fleming, Chérina; Brouwer, Rob; Lindemans, Jan; de Jonge, Robert

    2012-10-01

    We evaluated the body fluid (BF) module on the new Sysmex XN-1000 for counting blood cells. One hundred and eighty-seven BF samples [73 cerebrospinal fluid (CSF), 48 continuous ambulatory peritoneal dialysis (CAPD), 46 ascites, and 20 pleural fluid] were used for method comparison between the XN-1000 and manual microscopy (Fuchs-Rosenthal chamber and stained cytospin slides) for counting red blood cells (RBCs) and white blood cells (WBCs) (differential). Good agreement was found for counting WBCs (y=1.06x+0.09, n=67, R2=0.96) and mononuclear cells (MNs) (y=1.04x-0.01, n=40, R2=0.93) in CSF. However, the XN-1000 systematically counted more polymorphonuclear cells (PMNs) (y=1.48x+0.18, n=40, R2=0.99) compared to manual microscopy. Excellent correlation for RBCs >1×109/L (y=0.99x+116.56, n=26, R2=0.99) in CSF was found. For other fluids (CAPD, ascites and pleural fluid) excellent agreement was found for counting WBCs (y=1.06x+0.26, n=109, R2=0.98), MNs (y=1.06x-0.41, n=93, R2=0.96), PMNs (y=1.06x+0.81, n=93, R2=0.98) and RBCs (y=1.04x+110.04, n=43, R2=0.98). By using BF XN-check, the lower limit of quantitation (LLoQ) for WBC was defined at 5×106/L. Linearity was excellent for both the WBCs (R2=0.99) and RBCs (R2=0.99) and carry-over never exceeded 0.05%. The BF module on the XN-1000 is a suitable tool for fast and accurate quantification of WBC (differential) and RBC counts in CSF and other BFs in a diagnostic setting.

  2. Effect of the hemoglobin-based oxygen carrier HBOC-201 on laboratory instrumentation: cobas integra, chiron blood gas analyzer 840, Sysmex SE-9000 and BCT.

    PubMed

    Wolthuis, A; Peek, D; Scholten, R; Moreira, P; Gawryl, M; Clark, T; Westerhuis, L

    1999-01-01

    As part of a clinical trial, we evaluated the effects of the hemoglobin-based oxygen-carrier (HBOC) HBOC-201 (an ultrapurified, stroma-free bovine hemoglobin product, Biopure, Cambridge, MA, USA) on our routine clinical chemistry analyzer (Cobas Integra, F. Hoffmann-La Roche Ltd, Basel, Switzerland ), blood gas analyzer (Chiron 840, Chiron Diagnostics Corporation, East Walpole, MA, USA), routine hemocytometry analyzer (Sysmex SE-9000, TOA Medical Electronics Co Ltd., Kobe, Japan), hemostasis analyzer (BCT, Dade-Behring, Marburg, Germany) and bloodbanking system (Dia-Med-ID Micro Typing System, DiaMed AG, Cressier, Switzerland). The maximum tested concentration of HBOC-201 was 65 g/l. Of the 27 routine clinical chemistry tests challenged with HBOC-201, bilirubin-direct, creatine kinase MB-fraction (CK-MB), creatine kinase (CK), gamma-glutamyltransferase (GGT), magnesium and uric acid were influenced by even low concentrations of HBOC-201. These tests were excluded from use on the plasma of patients treated with HBOC-201. Since the non-availability of the cardiac marker CK-MB may lead to problems in acute situations, we introduced the qualitative Trop T-test (Boehringer Mannheim), which was not influenced. The applicability of another nine tests was limited by the concentration of the HBOC-201 in the patients' plasma. No interference of HBOC-201 in routine hemocytometry, hemostasis-analysis and red-blood cell agglutination detection (blood-bank tests) was observed. Although immediate patient-care was not compromised, routine use of hemoglobin-based oxygen carriers will have a strong impact on logistical management. The development of robust laboratory tests free from the interference of the pigmented oxygen carriers should therefore precede its introduction into routine transfusion medicine.

  3. Performance evaluation of the Sysmex pocH-100iV Diff hematology analyzer for analysis of canine, feline, equine, and bovine blood.

    PubMed

    Riond, Barbara; Weissenbacher, Stefan; Hofmann-Lehmann, Regina; Lutz, Hans

    2011-12-01

    The Sysmex pocH-100iV Diff is an impedance hematology analyzer recently introduced for point-of-care use in veterinary practices in Europe. The purpose of this study was to validate the pocH-100iV Diff for analysis of blood samples from dogs, cats, horses, and cattle. Fresh EDTA-blood samples from healthy and ill dogs (115), cats (94), horses (91), and cattle (78) were analyzed on the pocH-100iV Diff and the Cell-Dyn 3500. Results of the automated WBC differential counts were compared with the manual differential counts for 77 dogs, 65 cats, 40 horses, and 46 cattle. HCT were compared with PCVs obtained by microhematocrit centrifugation. Furthermore, precision, linearity, carry-over, cell aging, and clinical relevance of the pocH-100iV Diff results were assessed. Most of the CBC results obtained by the pocH-100iV Diff correlated well with those of the Cell-Dyn 3500. Slightly low correlation was observed for canine MCV and hemoglobin concentration. Lymphocytes correlated well in horses and cattle, but less well in cats and dogs. The mixed cell population termed "OTHRS" (all granulocytes and monocytes for horses and cattle; neutrophils, monocytes, and basophils for cats and dogs) correlated well in all tested species. The instrument overestimated feline and canine eosinophils. In cats, platelet counts showed a strong negative bias. The overall performance of the pocH-100iV Diff was excellent with the noted limitations. The automated differential count can be used as screening tool in conjunction with evaluation of a blood smear. © 2011 American Society for Veterinary Clinical Pathology.

  4. Flow-Induced Flutter of Multi-Inverted Flag Configurations: Vortex Dynamics and Flutter Behaviors

    NASA Astrophysics Data System (ADS)

    Rips, Aaron; Shoele, Kourosh; Mittal, Rajat

    2016-11-01

    Flow-induced flutter of "inverted" flags has potential application in energy-harvesting, enhanced mixing and heat transfer enhancement. While a number of previous studies have explored the dynamics of single inverted flags, the current study examines the issue of the coupled dynamics of multi-inverted flag configurations. The primary configurations investigated here have two inverted filaments in either side-by-side or tandem formations. The flapping behavior, dynamics, and flow-structure interaction of each filament as well as the synchronization between the dynamics of the filaments was studied. The investigation of the tandem configuration shows coupling of the flapping dynamics for a variety of separations as well as the ability of the trailing filament to drive the behavior of the leading filament. The behavior of the side-by-side configuration suggests the flapping dynamics in this configuration is dominated by the vortex shedding at the fixed trailing edges. The side-by-side configuration also exhibits multiple distinctive flutter behavior regimes depending on separation distance. Finally, simulations of flag formations with >2 flags are also explored. Implication of the findings on energy-harvesting applications is discussed. The authors would like to acknowledge financial support from NSF Grant CBET 1357819.

  5. Energy Harvesting for Micropower Applications by Flow-Induced Flutter of an Inverted Piezoelectric Flag

    NASA Astrophysics Data System (ADS)

    Shoele, Kourosh; Mittal, Rajat

    2015-11-01

    Piezoelectric flexible flags can be used to continuously generate energy for small-scale sensor used in a wide variety of applications ranging from measurement/monitoring of environmental conditions (outdoors or indoors) to in-situ tracking of wild animals. Here, we study the energy harvesting performance as well as the flow-structure interaction of an inverted piezoelectric flag. We use a coupled fluid-structure-electric solver to examine the dynamic response of the inverted flag as well as the associated vortical characteristics with different inertia and bending stiffness. Simulations indicate that large amplitude vibrations can be achieved over a large range of parameters over which lock-on between the flag flutter and the intrinsic wake shedding occurs. The effects of initial inclination of the flag to the prevailing flow as well as Reynolds number of the flow are explored, and the effect of piezoelectric material parameters on the energy harvesting performance of this flutter state is examined in detail. The maximum energy efficiency occurs when there is a match between the intrinsic timescales of flutter and the piezoelectric circuit. The simulations are used to formulate a scaling law that could be used to predict the energy harvesting performance of such devices. The support for this study comes from AFSOR, NSF, EPRI and Johns Hopkins E2SHI Seed Grant.

  6. Utility of the Technicon H-1 flags in the detection of peripheral blood blast cells of paediatric acute leukaemia patients.

    PubMed

    Fernández-Castro, M; Viloria, A

    1995-01-01

    This study evaluates the sensitivity and positive predictive value of some of the morphological flags supplied by the Technicon H-1 haematological analyser to detect circulating blasts. A total of 1,269 venous blood samples from 91 paediatric patients diagnosed of acute leukemia were analysed in the Technicon H-1. A conventional microscopic manual method was used for reference. The prevalence was 17.7% for the presence of blasts. The results showed that all samples with more than 5% blasts were detected by some WBC morphological flag: only 5 samples (2.2%) with less than 5% blasts showed no morphological flag. In most cases (86%), the flags observed were either the 'Atyp' flag or the 'Blast' flag or both simultaneously. The positive predictive value obtained by the 'Blast' flag for the true presence of blasts was 66.8%, and for the 'Atyp' flag 34.9%; it was 75.8% when both flags were present simultaneously. False positives were associated with postchemotherapy monocytosis or severe neutropenia.

  7. What's in a flag? Subliminal exposure to New Zealand national symbols and the automatic activation of egalitarian versus dominance values.

    PubMed

    Sibley, Chris G; Hoverd, William James; Duckitt, John

    2011-01-01

    Three experiments tested whether the subliminal presentation of national symbols automatically elicited societally prescribed normative values in the New Zealand (NZ) context using a lexical decision task. Consistent with research in the United States, the presentation of the NZ flag (Study 1), but not another consensually validated NZ national symbol (The Silver Fern, Study 2), increased the cognitive accessibility of egalitarian value concepts. The NZ flag did not, however, activate values in a comparable sample of foreign nationals (Study 3). National flags, it seems, automatically activate normative values for ingroup members, and this effect is not limited to nations with a high frequency of flag-display behavior such as the United States.

  8. Bernard J. Cigrand, DDS: Father of Flag Day and renaissance man.

    PubMed

    Spiegel, Allen D; Kavaler, Florence

    2007-06-01

    Dr. Bernard J. Cigrand is acknowledged as the "Father of Flag Day." He relentlessly continued his activities for more than sixty years to have June 14 designated for the national observance of the birth of the American flag. That finally occurred in 1948, seventeen years after his death, when President Harry S. Truman signed a Congressional Act into law. However, the law designated a voluntary observance but did not create a legal national holiday. In addition to his fervent passion for Flag Day, Cigrand undertook a variety of other initiatives He was a practicing dentist, the dean of a dental school, an investigative journalist, an expert on heraldry and seals, a lecturer and an author of books. Despite all his achievements, Cigrand is more widely known in the European country from where his parents emigrated to the United States in 1852 - Luxembourg, than in the United States.

  9. Why do inverted-flags flap in a uniform steady flow?

    NASA Astrophysics Data System (ADS)

    Sader, John; Cossé, Julia; Kim, Daegyoum; Fan, Boyu; Gharib, Morteza

    2016-11-01

    The dynamics of a cantilevered elastic sheet, with a uniform steady flow impinging on its clamped-end, have been studied widely and provide insight into the stability of flags and biological phenomena. Recent measurements by Kim et al. show that reversing the sheet's orientation, with the flow impinging on its free-edge, dramatically alters its dynamics. In this talk, we use a combination of mathematical theory, scaling analysis and measurement to explore the physical mechanisms driving the observed large-amplitude flapping motion of an inverted-flag. Flapping is found to be periodic predominantly, with a transition to chaos as flow speed increases. These findings have implications to leaf motion and other biological processes, such as the dynamics of individual hairs, because they also can present an inverted-flag configuration. Gordon and Betty Moore Foundation; Ministry of Trade, Industry and Energy, Republic of Korea; Australian Research Council Grants scheme.

  10. Synthesis and materialization of a reaction-diffusion French flag pattern

    NASA Astrophysics Data System (ADS)

    Zadorin, Anton S.; Rondelez, Yannick; Gines, Guillaume; Dilhas, Vadim; Urtel, Georg; Zambrano, Adrian; Galas, Jean-Christophe; Estevez-Torres, André

    2017-10-01

    During embryo development, patterns of protein concentration appear in response to morphogen gradients. These patterns provide spatial and chemical information that directs the fate of the underlying cells. Here, we emulate this process within non-living matter and demonstrate the autonomous structuration of a synthetic material. First, we use DNA-based reaction networks to synthesize a French flag, an archetypal pattern composed of three chemically distinct zones with sharp borders whose synthetic analogue has remained elusive. A bistable network within a shallow concentration gradient creates an immobile, sharp and long-lasting concentration front through a reaction-diffusion mechanism. The combination of two bistable circuits generates a French flag pattern whose 'phenotype' can be reprogrammed by network mutation. Second, these concentration patterns control the macroscopic organization of DNA-decorated particles, inducing a French flag pattern of colloidal aggregation. This experimental framework could be used to test reaction-diffusion models and fabricate soft materials following an autonomous developmental programme.

  11. [The dorsal flag flap for alternative skin coverage for defects of the thumb tip: case reports].

    PubMed

    Stang, F; Rab, M; van Schoonhoven, J; Prommersberger, K-J

    2008-04-01

    This case report describes the use of the dorsal flag flap in contrast to its standard indication (dorsal and palmar defects of the proximal finger phalanx) for defect coverage of the thumb tip in three patients. In 2006, defects of the thump tip were covered with a dorsal flag flap in three male cases. After 6 - 9 months a clinical evaluation was performed with special focus on pain, aesthetic aspect, sensibility and function. Primary wound healing without complications was observed in all cases. No functional impairments or pain have been reported, the aesthetic aspect was satisfying for the patients. All flaps showed an intact protection sensibility (in terms of pressure and pain perception). The subjective shortcoming of the sensitivity like numbness was not distracting to any of the patients. The dorsal flag flap is a good alternative for covering tissue defects at the thumb tip compared to local flap grafts or neurovascular long-distance flaps with satisfying results.

  12. The flagellar protein FLAG1/SMP1 is a candidate for Leishmania-sand fly interaction.

    PubMed

    Di-Blasi, Tatiana; Lobo, Amanda R; Nascimento, Luanda M; Córdova-Rojas, Jose L; Pestana, Karen; Marín-Villa, Marcel; Tempone, Antonio J; Telleria, Erich L; Ramalho-Ortigão, Marcelo; McMahon-Pratt, Diane; Traub-Csekö, Yara M

    2015-03-01

    Leishmaniasis is a serious problem that affects mostly poor countries. Various species of Leishmania are the agents of the disease, which take different clinical manifestations. The parasite is transmitted by sandflies, predominantly from the Phlebotomus genus in the Old World and Lutzomyia in the New World. During development in the gut, Leishmania must survive various challenges, which include avoiding being expelled with blood remnants after digestion. It is believed that attachment to the gut epithelium is a necessary step for vector infection, and molecules from parasites and sand flies have been implicated in this attachment. In previous work, monoclonal antibodies were produced against Leishmania. Among these an antibody was obtained against Leishmania braziliensis flagella, which blocked the attachment of Leishmania panamensis flagella to Phlebotomus papatasi guts. The protein recognized by this antibody was identified and named FLAG1, and the complete FLAG1 gene sequence was obtained. This protein was later independently identified as a small, myristoylated protein and called SMP1, so from now on it will be denominated FLAG1/SMP1. The FLAG1/SMP1 gene is expressed in all developmental stages of the parasite, but has higher expression in promastigotes. The anti-FLAG1/SMP1 antibody recognized the flagellum of all Leishmania species tested and generated the expected band by western blots. This antibody was used in attachment and infection blocking experiments. Using the New World vector Lutzomyia longipalpis and Leishmania infantum chagasi, no inhibition of attachment ex vivo or infection in vivo was seen. On the other hand, when the Old World vectors P. papatasi and Leishmania major were used, a significant decrease of both attachment and infection were seen in the presence of the antibody. We propose that FLAG1/SMP1 is involved in the attachment/infection of Leishmania in the strict vector P. papatasi and not the permissive vector L. longipalpis.

  13. Multi-Level Pre-Correlation RFI Flagging for Real-Time Implementation on UniBoard

    NASA Astrophysics Data System (ADS)

    Dumez-Viou, Cédric; Weber, Rodolphe; Ravier, Philippe

    2016-03-01

    Because of the denser active use of the spectrum, and because of radio telescopes higher sensitivity, radio frequency interference (RFI) mitigation has become a sensitive topic for current and future radio telescope designs. Even if quite sophisticated approaches have been proposed in the recent years, the majority of RFI mitigation operational procedures are based on post-correlation corrupted data flagging. Moreover, given the huge amount of data delivered by current and next generation radio telescopes, all these RFI detection procedures have to be at least automatic and, if possible, real-time. In this paper, the implementation of a real-time pre-correlation RFI detection and flagging procedure into generic high-performance computing platforms based on field programmable gate arrays (FPGA) is described, simulated and tested. One of these boards, UniBoard, developed under a Joint Research Activity in the RadioNet FP7 European programme is based on eight FPGAs interconnected by a high speed transceiver mesh. It provides up to 4 TMACs with ®Altera Stratix IV FPGA and 160 Gbps data rate for the input data stream. The proposed concept is to continuously monitor the data quality at different stages in the digital preprocessing pipeline between the antennas and the correlator, at the station level and the core level. In this way, the detectors are applied at stages where different time-frequency resolutions can be achieved and where the interference-to-noise ratio (INR) is maximum right before any dilution of RFI characteristics by subsequent channelizations or signal recombinations. The detection decisions could be linked to a RFI statistics database or could be attached to the data for later stage flagging. Considering the high in-out data rate in the pre-correlation stages, only real-time and go-through detectors (i.e. no iterative processing) can be implemented. In this paper, a real-time and adaptive detection scheme is described. An ongoing case study has been

  14. Large Crater Repair at Silver Flag Exercise Site, Tyndall Air Force Base, Florida

    DTIC Science & Technology

    2015-08-01

    Site, Tyndall Air Force Base , Florida G eo te ch ni ca l a nd S tr uc tu re s La bo ra to ry William D. Carruth, Lulu Edwards, Haley P. Bell...ERDC/GSL TR-15-27 August 2015 Large Crater Repair at Silver Flag Exercise Site, Tyndall Air Force Base , Florida William D. Carruth, Haley P. Bell...ft) craters at the Silver Flag Exercise Site in Tyndall Air Force Base , Florida. Modernized ADR technologies were successfully demonstrated in a

  15. A Radio Visibility Data Auto-Flag Method Based on Support Vector Machine

    NASA Astrophysics Data System (ADS)

    Dai, H. M.; Mei, Y.; Wang, W.; Deng, H.; Wang, F.

    2016-01-01

    The Mingantu Ultrawide Spectral Radioheliograph (MUSER) has entered the trial observation stage. After the construction of data acquisition and real-time storage system, it is urgent to automatically flag and eliminate abnormal visibility data so as to improve the image quality. In this paper, according to the observational records, we create a credible visibility set, and further obtain a corresponding model by using support vector machine (SVM) technology. The results show that the SVM is a robust approach to flag the MUSER visibility data, and could reach the accuracy of about 86%. Meanwhile, the approach would not be affected by solar activities such as flare eruptions.

  16. Close-up view of U.S. flag deployed on Moon by Apollo 17 crew

    NASA Image and Video Library

    1972-12-12

    AS17-134-20466 (7-19 Dec. 1972) --- A close-up view of the U.S. flag deployed on the moon at the Taurus-Littrow landing site by the crewmen of the Apollo 17 lunar landing mission. The crescent Earth can be seen in the far distant background above the flag. The lunar feature in the near background is South Massif. While astronauts Eugene A. Cernan and Harrison H. Schmitt descended in the Lunar Module "Challenger" to explore the lunar surface, astronaut Ronald E. Evans remained with the Apollo 17 Command and Service Modules in lunar orbit.

  17. AsteroFLAG: First results from hare-and-hounds Exercise #1

    NASA Astrophysics Data System (ADS)

    Chaplin, W. J.; Appourchaux, T.; Arentoft, T.; Ballot, J.; Christensen-Dalsgaard, J.; Creevey, O. L.; Elsworth, Y.; Fletcher, S. T.; García, R. A.; Houdek, G.; Jiménez-Reyes, S. J.; Kjeldsen, H.; New, R.; Régulo, C.; Salabert, D.; Sekii, T.; Sousa, S. G.; Toutain, T.; rest of asteroFLAG Group

    2008-06-01

    We report on initial results from the first phase of Exercise #1 of the asteroFLAG hare and hounds. The asteroFLAG group is helping to prepare for the asteroseismology component of NASA's Kepler mission, and the first phase of Exercise #1 is concerned with testing extraction of estimates of the large and small frequency spacings of the low-degree p modes from Kepler-like artificial data. These seismic frequency spacings will provide key input for complementing the exoplanet search data.

  18. (U) A Gruneisen Equation of State for TPX. Application in FLAG

    SciTech Connect

    Fredenburg, David A.; Aslam, Tariq Dennis; Bennett, Langdon Stanford

    2015-11-02

    A Gruneisen equation of state (EOS) is developed for the polymer TPX (poly 4-methyl-1-pentene) within the LANL hydrocode FLAG. Experimental shock Hugoniot data for TPX is fit to a form of the Gruneisen EOS, and the necessary parameters for implementing the TPX EOS in FLAG are presented. The TPX EOS is further validated through one-dimensional simulations of recent double-shock experiments, and a comparison is made between the new Gruneisen EOS for TPX and the EOS representation for TPX used in the LANL Common Model.

  19. Astronaut David Scott gives salute beside U.S. flag during EVA

    NASA Technical Reports Server (NTRS)

    1971-01-01

    Astronaut David R. Scott, commander, gives a military salute while standing beside the deployed U.S. flag during the Apollo 15 lunar surface extravehicular activity (EVA) at the Hadley-Apennine landing site. The flag was deployed toward the end of EVA-2. The Lunar Module 'Falcon' is partially visible on the right. Hadley Delta in the background rises approximately 4,000 meters (about 13,124 feet) above the plain. The base of the mountain is approximately 5 kilometers (about 3 statute miles) away. This photograph was taken by Astronaut James B. Irwin, Lunar Module pilot.

  20. Astronaut James Irwin gives salute beside U.S. flag during EVA

    NASA Technical Reports Server (NTRS)

    1971-01-01

    Astronaut James B. Irwin, lunar module pilot, gives a military salute while standing beside the deployed U.S. flag during the Apollo 15 lunar surface extravehicular activity (EVA) at the Hadley-Apennine landing site. The flag was deployed toward the end of EVA-2. The Lunar Module 'Falcon' is partially visible on the right. Hadley Delta in the background rises approximately 4,000 meters (about 13,124 feet) above the plain. The base of the mountain is approximately 5 kilometers (about 3 statute miles) away. This photograph was taken by Astronaut David R. Scott, Apollo 15 commander.

  1. solarFLAG hare and hounds: estimation of p-mode frequencies from Sun-as-star helioseismology data

    NASA Astrophysics Data System (ADS)

    Jiménez-Reyes, S. J.; Chaplin, W. J.; García, R. A.; Appourchaux, T.; Baudin, F.; Boumier, P.; Elsworth, Y.; Fletcher, S. T.; Lazrek, M.; Leibacher, J. W.; Lochard, J.; New, R.; Régulo, C.; Salabert, D.; Toutain, T.; Verner, G. A.; Wachter, R.

    2008-10-01

    We report on the results of the latest solarFLAG hare-and-hounds exercise, which was concerned with testing methods for extraction of frequencies of low-degree solar p modes from data collected by Sun-as-a-star observations. We have used the new solarFLAG simulator, which includes the effects of correlated mode excitation and correlations with background noise, to make artificial time-series data that mimic Doppler velocity observations of the Sun-as-a-star. The correlations give rise to asymmetry of mode peaks in the frequency power spectrum. 10 members of the group (the hounds) applied their `peak-bagging' codes to a 3456-d data set, and the estimated mode frequencies were returned to the hare (who was WJC) for comparison. Analysis of the results reveals a systematic bias in the estimated frequencies of modes above ~1.8mHz. The bias is negative, meaning the estimated frequencies systematically underestimate the input frequencies. We identify two sources that are the dominant contributions to the frequency bias. Both sources involve failure to model accurately subtle aspects of the observed power spectral density in the part (window) of the frequency power spectrum that is being fitted. One source of bias arises from a failure to account for the power spectral density coming from all those modes whose frequencies lie outside the fitting windows. The other source arises from a failure to account for the power spectral density of the weak l = 4 and 5 modes, which are often ignored in Sun-as-a-star analysis. The Sun-as-a-star peak-bagging codes need to allow for both sources, otherwise the frequencies are likely to be biased.

  2. 14 CFR 121.467 - Flight attendant duty period limitations and rest requirements: Domestic, flag, and supplemental...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... time, using Coordinated Universal Time or local time, that begins at midnight and ends 24 hours later... domestic, flag, or supplemental operations. The time is calculated using either Coordinated Universal Time..., FLAG, AND SUPPLEMENTAL OPERATIONS Aircraft Dispatcher Qualifications and Duty Time...

  3. 14 CFR 121.467 - Flight attendant duty period limitations and rest requirements: Domestic, flag, and supplemental...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... time, using Coordinated Universal Time or local time, that begins at midnight and ends 24 hours later... domestic, flag, or supplemental operations. The time is calculated using either Coordinated Universal Time..., FLAG, AND SUPPLEMENTAL OPERATIONS Aircraft Dispatcher Qualifications and Duty Time...

  4. 41 CFR 301-10.132 - Who is required to use a U.S. flag air carrier?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... required to use a U.S. flag air carrier? Anyone whose air travel is financed by U.S. Government funds... U.S. flag air carrier? 301-10.132 Section 301-10.132 Public Contracts and Property Management Federal Travel Regulation System TEMPORARY DUTY (TDY) TRAVEL ALLOWANCES ALLOWABLE TRAVEL EXPENSES 10...

  5. 41 CFR 301-10.132 - Who is required to use a U.S. flag air carrier?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... required to use a U.S. flag air carrier? Anyone whose air travel is financed by U.S. Government funds... U.S. flag air carrier? 301-10.132 Section 301-10.132 Public Contracts and Property Management Federal Travel Regulation System TEMPORARY DUTY (TDY) TRAVEL ALLOWANCES ALLOWABLE TRAVEL EXPENSES 10...

  6. 46 CFR 39.1015 - Foreign-flagged tank vessel certification procedures for vapor control system designs-TB/ALL.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... certification by the classification society that classifies vessels under their foreign flags to the Marine... requirements of this part, the Officer in Charge, Marine Inspection (OCMI) will endorse the vessel's... 46 Shipping 1 2014-10-01 2014-10-01 false Foreign-flagged tank vessel certification procedures...

  7. 46 CFR 39.1015 - Foreign-flagged tank vessel certification procedures for vapor control system designs-TB/ALL.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... certification by the classification society that classifies vessels under their foreign flags to the Marine... requirements of this part, the Officer in Charge, Marine Inspection (OCMI) will endorse the vessel's... 46 Shipping 1 2013-10-01 2013-10-01 false Foreign-flagged tank vessel certification procedures...

  8. Rates of false flagging due to statistical artifact in CMS evaluations of transplant programs: results of a stochastic simulation.

    PubMed

    Massie, A B; Segev, D L

    2013-08-01

    The recent CMS conditions of participation are based on risk-adjusted models produced by the Scientific Registry for Transplant Recipients (SRTR). The accuracy of these models in identifying poor-performing centers is unknown. In this stochastic simulation study, 1-year mortality outcomes were simulated in virtual transplant centers, and used to flag centers according to the methods used by CMS, evaluating nine overlapping 2.5-year periods of simulated data. In a simulation where all centers had the same underlying risk, 10.2% were falsely flagged at least once during the 4.5 years of simulated evaluations. The probability of false-positive flagging was lowest in low-volume centers (2.5%) and highest in high-volume centers (16.2%). In another simulation where 5% of centers were assigned twofold risk ("poor-performing centers"), only 32% of poor-performing centers were correctly flagged. In a final simulation where each center was assigned a unique mortality risk, 94% of flagged centers had greater-than-median risk, but only 32% of flagged centers were among the 5% with highest risk. Even after disregarding known covariate limitations to the risk adjustment models, statistical noise alone leads to spurious flagging of many adequately-performing transplant centers, yet the methods used by CMS fail to flag most centers with true elevated risk. © Copyright 2013 The American Society of Transplantation and the American Society of Transplant Surgeons.

  9. 48 CFR 47.403-1 - Availability and unavailability of U.S.-flag air carrier service.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Availability and unavailability of U.S.-flag air carrier service. 47.403-1 Section 47.403-1 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT TRANSPORTATION Air Transportation by U.S.-Flag Carriers...

  10. Switchgrass (Panicum virgatum L) flag leaf transcriptomes reveal molecular signatures of leaf development, senescence, and mineral dynamics

    USDA-ARS?s Scientific Manuscript database

    We provide the first comprehensive transcriptomic inspection of switchgrass flag leaf development. Flag leaves were collected from field grown switchgrass plants at five plant developmental stages: heading, anthesis, early and late seed development, and at physiological maturity and analyzed by RNA...

  11. 50 CFR 216.46 - U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 10 2013-10-01 2013-10-01 false U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation Program. 216.46 Section 216.46 Wildlife and Fisheries....46 U.S. citizens on foreign flag vessels operating under the International Dolphin...

  12. 50 CFR 216.46 - U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 10 2012-10-01 2012-10-01 false U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation Program. 216.46 Section 216.46 Wildlife and Fisheries....46 U.S. citizens on foreign flag vessels operating under the International Dolphin...

  13. 50 CFR 216.46 - U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation Program. 216.46 Section 216.46 Wildlife and Fisheries....46 U.S. citizens on foreign flag vessels operating under the International Dolphin...

  14. 50 CFR 216.46 - U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 10 2014-10-01 2014-10-01 false U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation Program. 216.46 Section 216.46 Wildlife and Fisheries....46 U.S. citizens on foreign flag vessels operating under the International Dolphin...

  15. 50 CFR 216.46 - U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false U.S. citizens on foreign flag vessels operating under the International Dolphin Conservation Program. 216.46 Section 216.46 Wildlife and Fisheries....46 U.S. citizens on foreign flag vessels operating under the International Dolphin...

  16. 76 FR 1665 - Stakeholder Meetings Regarding the U.S.-Flag Great Lakes Fleet Revitalization Study; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-11

    ... Administration's U.S.-Flag Great Lakes Fleet Revitalization Study. MARAD inadvertently listed the incorrect time... Maritime Administration Stakeholder Meetings Regarding the U.S.-Flag Great Lakes Fleet Revitalization Study; Correction AGENCY: Maritime Administration, Department of Transportation. ACTION: Correction Notice. SUMMARY...

  17. An unusual case of seed dispersal in an invasive aquatic; yellow flag iris (Iris pseudacorus)

    USDA-ARS?s Scientific Manuscript database

    Understanding reproductive mode of invasive plants can help managers plan more efficacious control. Invasive aquatics typically reproduce primarily through vegetative means. Yellow flag iris is an invasive plant species often growing as an emergent aquatic. There have been contradictory reports of i...

  18. Raising FLAGS: Renewing Core French at the Pre-Service Teacher Level

    ERIC Educational Resources Information Center

    Carr, Wendy

    2010-01-01

    A new program for core French teacher candidates called FLAGS (French Language and Global Studies) was established at the University of British Columbia (UBC) in 2007. The program is intended for those who are keen to teach core French and possess rudimentary proficiency in the language but may not necessarily have the same proficiency or prior…

  19. Immunohistochemical Detection of FLAG-Tagged Endogenous Proteins in Knock-In Mice

    PubMed Central

    Newton, Kim; Chu, Felix; Webster, Joshua D.; French, Dorothy M.

    2015-01-01

    With recent advances in immunohistochemical (IHC) techniques, immunohistochemistry now plays a more important role in research, especially in mouse models where characterization of cellular patterns of protein expression has become critical. Even with these recent advances, a paucity of IHC quality antibodies for some proteins still exists. To address this, we have developed a novel IHC assay that utilizes a commercially available goat anti-DDDDK peptide polyclonal antibody on paraffin-embedded tissues from knock-in mice expressing proteins of interest tagged with a 3×FLAG epitope at physiologically relevant levels. Focusing on two 3×FLAG-tagged proteins for which specific antibodies were available, USP48 and RIPK3, we were able to validate our anti-DDDDK assay by comparing the IHC directed against the actual proteins to the anti-DDDDK IHC assay, which recognizes the FLAG epitope. We were also able to detect a third 3×FLAG-tagged protein, BAP1, for which quality reagents were not available. This universal IHC method will enable researchers to characterize the expression patterns of proteins of interest when specific antibodies are lacking. PMID:25575566

  20. Constructing and Validating the Foreign Language Attitudes and Goals Survey (FLAGS)

    ERIC Educational Resources Information Center

    Cid, Eva; Granena, Gisela; Tragant, Elsa

    2009-01-01

    The present study describes the process that was followed in the construction and validation of the foreign language attitudes and goals survey (FLAGS), a new questionnaire based on qualitative data from Tragant and Munoz [Tragant, Munoz, C., 2000. "La motivacion y su relacion con la edad en un contexto escolar de aprendizaje de una lengua…

  1. 77 FR 72712 - Identity Theft Red Flags and Address Discrepancies Under the Fair and Accurate Credit...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-06

    ..., Consumer reporting agencies, Credit, Creditors, Fair credit, Information furnishers, Identity theft, Trade... From the Federal Register Online via the Government Publishing Office FEDERAL TRADE COMMISSION 16 CFR Part 681 RIN 3084-AA94 Identity Theft Red Flags and Address Discrepancies Under the Fair and...

  2. Flagging versus dragging as sampling methods for nymphal Ixodes scapularis (Acari: Ixodidae)

    USGS Publications Warehouse

    Rulison, Eric L.; Kuczaj, Isis; Pang, Genevieve; Hickling, Graham J.; Tsao, Jean I.; Ginsberg, Howard S.

    2013-01-01

    The nymphal stage of the blacklegged tick, Ixodes scapularis (Acari: Ixodidae), is responsible for most transmission of Borrelia burgdorferi, the etiologic agent of Lyme disease, to humans in North America. From 2010 to fall of 2012, we compared two commonly used techniques, flagging and dragging, as sampling methods for nymphal I. scapularis at three sites, each with multiple sampling arrays (grids), in the eastern and central United States. Flagging and dragging collected comparable numbers of nymphs, with no consistent differences between methods. Dragging collected more nymphs than flagging in some samples, but these differences were not consistent among sites or sampling years. The ratio of nymphs collected by flagging vs dragging was not significantly related to shrub density, so habitat type did not have a strong effect on the relative efficacy of these methods. Therefore, although dragging collected more ticks in a few cases, the numbers collected by each method were so variable that neither technique had a clear advantage for sampling nymphal I. scapularis.

  3. Psychometric Analysis of the Systematic Observation of Red Flags for Autism Spectrum Disorder in Toddlers

    ERIC Educational Resources Information Center

    Dow, Deanna; Guthrie, Whitney; Stronach, Sheri T.; Wetherby, Amy M.

    2017-01-01

    The purpose of this study was to examine the utility of the Systematic Observation of Red Flags as an observational level-two screening measure to detect risk for autism spectrum disorder in toddlers when used with a video-recorded administration of the Communication and Symbolic Behavior Scales. Psychometric properties of the Systematic…

  4. 38 CFR 1.9 - Description, use, and display of VA seal and flag.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) Replica means a copy of the official seal displaying the identical form, content, and colors thereof. (5... gold, brown, blue, white, silver, yellow, black, and red. (iv) The colors are derived from the American... thereof. (ii) A Class 1 flag shall be of nylon banner, measure 4′4″ on the hoist by 5′6″ on the...

  5. 38 CFR 1.9 - Description, use, and display of VA seal and flag.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) Replica means a copy of the official seal displaying the identical form, content, and colors thereof. (5... gold, brown, blue, white, silver, yellow, black, and red. (iv) The colors are derived from the American... thereof. (ii) A Class 1 flag shall be of nylon banner, measure 4′4″ on the hoist by 5′6″ on the...

  6. Phloem Transport Of Arsenic Species From Flag Leaf To Grain During Grain Filling

    EPA Science Inventory

    Strategies to reduce arsenic (As) in rice grain, below concentrations that represent a serious human health concern, require that the mechanisms of As accumulation within grain be established. Therefore, retranslocation of As species from flag leaves into filling rice grain was ...

  7. Phloem transport of arsenic species from flag leaf to grain during grain filling

    PubMed Central

    Carey, Anne-Marie; Norton, Gareth J.; Deacon, Claire; Scheckel, Kirk G.; Lombi, Enzo; Punshon, Tracy; Guerinot, Mary Lou; Lanzirotti, Antonio; Newville, Matt; Choi, Yongseong; Price, Adam H.; Meharg, Andrew A.

    2013-01-01

    Summary Strategies to reduce arsenic in rice grain, below levels that represent a serious human health concern, require that the mechanisms of arsenic accumulation within grain be established. Therefore, re-translocation of arsenic species from flag leaves into filling rice grain was investigated.Arsenic species were delivered through cut flag leaves during grain fill. Spatial unloading within grains was investigated using synchrotron X-ray fluorescence (SXRF) microtomography. Additionally, the effect of germanic acid (a silicic acid analogue) on grain arsenic accumulation in arsenite treated panicles was examined.Dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) were extremely efficiently re-translocated from flag leaves to rice grain; arsenate was poorly re-translocated, and was rapidly reduced to arsenite within flag leaves; arsenite displayed no re-translocation. Within grains, DMA rapidly dispersed while MMA and inorganic arsenic remained close to the entry point. Germanic acid addition did not affect grain arsenic in arsenite treated panicles. 3D SXRF microtomography gave further information on arsenite localization in the ovular vascular trace (OVT) of rice grains.These results demonstrate that inorganic arsenic is poorly re-mobilized, while organic species are readily re-mobilized, from leaves to grain. Stem translocation of inorganic arsenic may not rely solely on silicic acid transporters. PMID:21658183

  8. 22 CFR 201.15 - U.S. flag vessel shipping requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... COMMODITY TRANSACTIONS FINANCED BY USAID Conditions Governing the Eligibility of Procurement Transactions... and tankers shall be achieved for each quantitative unit of cargo. A quantitative unit of cargo is the... determined that at least 50% of the quantitative unit will move on U.S. flag vessels, to the extent that...

  9. 46 CFR 154.22 - Foreign flag vessel: Certificate of Compliance endorsement application.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... vessel that does not meet § 154.12 (b), (c), or (d)— (i) A certification from a classification society... by the Commanding Officer, Marine Safety Center to determine whether or not the vessel meets this... 46 Shipping 5 2012-10-01 2012-10-01 false Foreign flag vessel: Certificate of...

  10. 46 CFR 154.22 - Foreign flag vessel: Certificate of Compliance endorsement application.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... classification society that the vessel— (A) Has enhanced grades of steel meeting § 154.170 (b)(1) and (b)(2) for... 46 Shipping 5 2014-10-01 2014-10-01 false Foreign flag vessel: Certificate of Compliance... (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING...

  11. 46 CFR 154.22 - Foreign flag vessel: Certificate of Compliance endorsement application.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... classification society that the vessel— (A) Has enhanced grades of steel meeting § 154.170 (b)(1) and (b)(2) for... 46 Shipping 5 2013-10-01 2013-10-01 false Foreign flag vessel: Certificate of Compliance... (CONTINUED) CERTAIN BULK DANGEROUS CARGOES SAFETY STANDARDS FOR SELF-PROPELLED VESSELS CARRYING...

  12. 46 CFR 154.22 - Foreign flag vessel: Certificate of Compliance endorsement application.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... vessel that does not meet § 154.12 (b), (c), or (d)— (i) A certification from a classification society... by the Commanding Officer, Marine Safety Center to determine whether or not the vessel meets this... 46 Shipping 5 2011-10-01 2011-10-01 false Foreign flag vessel: Certificate of...

  13. 14 CFR § 1221.113 - Use of the NASA Flags.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Use of the NASA Flags. § 1221.113 Section § 1221.113 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA...

  14. 14 CFR § 1221.106 - Establishment of the NASA Flag.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Establishment of the NASA Flag. § 1221.106 Section § 1221.106 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION THE NASA SEAL AND OTHER DEVICES, AND THE CONGRESSIONAL SPACE MEDAL OF HONOR NASA Seal, NASA Insignia, NASA Logotype, NASA...

  15. 14 CFR 121.99 - Communications facilities-domestic and flag operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Communications facilities-domestic and flag operations. 121.99 Section 121.99 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... system must be able to provide immediate satellite-based voice communications of...

  16. 14 CFR 121.711 - Communication records: Domestic and flag operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... operations. 121.711 Section 121.711 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Records and Reports § 121.711.... Effective Date Note: By Amdt. 121-366, 78 FR 67841, Nov. 12, 2013, § 121.711 was revised, effective Mar. 12...

  17. Genome Sequences of Cluster G Mycobacteriophages Cambiare, FlagStaff, and MOOREtheMARYer

    PubMed Central

    Augustine, David A.; Carroll, Daniel C.; Duncan, Julia C.; Harwi, Kyle M.; Howry, Rachel; Jagessar, Bhavita; Lum, Brandon A.; Meinert, Justin W.; Migliozzi, Jeffrey S.; Milliken, Katherine A.; Mitchell, Chandler J.; Nalatwad, Akanksha S.; Orlandini, Kaila C.; Rhein, Michael J.; Saravanan, Vishmayaa; Seese, Brett A.; Schiebel, Johnathon G.; Thomas, Kayla B.; Adkins, Nancy L.; Cohen, Karen L.; Iyengar, Varun B.; Kim, Hannah; Kramer, Zachary J.; Montgomery, Matthew T.; Schafer, Claire E.; Wilkes, Kellyn E.; Grubb, Sarah R.; Warner, Marcie H.; Bowman, Charles A.; Russell, Daniel A.; Hatfull, Graham F.

    2015-01-01

    Mycobacteriophages Cambiare, FlagStaff, and MOOREtheMARYer are newly isolated phages of Mycobacterium smegmatis mc2 155 recovered from soil samples in Pittsburgh, PA. All three genomes are closely related to cluster G mycobacteriophages but differ sufficiently in nucleotide sequence and gene content to warrant division of cluster G into several subclusters. PMID:26089410

  18. Constructing and Validating the Foreign Language Attitudes and Goals Survey (FLAGS)

    ERIC Educational Resources Information Center

    Cid, Eva; Granena, Gisela; Tragant, Elsa

    2009-01-01

    The present study describes the process that was followed in the construction and validation of the foreign language attitudes and goals survey (FLAGS), a new questionnaire based on qualitative data from Tragant and Munoz [Tragant, Munoz, C., 2000. "La motivacion y su relacion con la edad en un contexto escolar de aprendizaje de una lengua…

  19. [Patriotism and the American Flag. Articles Compiled from Nine Issues of "Instructor" and "Grade Teacher Magazines.

    ERIC Educational Resources Information Center

    San Mateo County Office of Education, Redwood City, CA. SMERC Information Center.

    Materials and activities for teaching about patriotism, the American flag, and the Pledge of Allegiance are offered in this compilation of articles taken from issues of "Grade Teacher" (Feb. 1952, Jun. 1954, Feb. 1958, Jun. 1960, Jun. 1962, Sep. 1971), and "Instructor" (Jun. 1962, Feb. 1975, Feb. 1983), magazines. The…

  20. Phloem Transport Of Arsenic Species From Flag Leaf To Grain During Grain Filling

    EPA Science Inventory

    Strategies to reduce arsenic (As) in rice grain, below concentrations that represent a serious human health concern, require that the mechanisms of As accumulation within grain be established. Therefore, retranslocation of As species from flag leaves into filling rice grain was ...

  1. Dynamic Black-Level Correction and Artifact Flagging in the Kepler Data Pipeline

    NASA Technical Reports Server (NTRS)

    Clarke, B. D.; Kolodziejczak, J. J.; Caldwell, D. A.

    2013-01-01

    Instrument-induced artifacts in the raw Kepler pixel data include time-varying crosstalk from the fine guidance sensor (FGS) clock signals, manifestations of drifting moiré pattern as locally correlated nonstationary noise and rolling bands in the images which find their way into the calibrated pixel time series and ultimately into the calibrated target flux time series. Using a combination of raw science pixel data, full frame images, reverse-clocked pixel data and ancillary temperature data the Keplerpipeline models and removes the FGS crosstalk artifacts by dynamically adjusting the black level correction. By examining the residuals to the model fits, the pipeline detects and flags spatial regions and time intervals of strong time-varying blacklevel (rolling bands ) on a per row per cadence basis. These flags are made available to downstream users of the data since the uncorrected rolling band artifacts could complicate processing or lead to misinterpretation of instrument behavior as stellar. This model fitting and artifact flagging is performed within the new stand-alone pipeline model called Dynablack. We discuss the implementation of Dynablack in the Kepler data pipeline and present results regarding the improvement in calibrated pixels and the expected improvement in cotrending performances as a result of including FGS corrections in the calibration. We also discuss the effectiveness of the rolling band flagging for downstream users and illustrate with some affected light curves.

  2. The Ultimate Flag Games Curriculum: An Answer to a Participation Problem

    ERIC Educational Resources Information Center

    Bell, Martie

    2011-01-01

    In response to a lack of active participation by students in physical education, the Ultimate Flag Games curriculum was created and has thrived in the decades since its inception. The curriculum has been successful at the middle school, high school, and higher education levels. This article introduced the curriculum, different games, and rules and…

  3. Eight Stars of Gold--The Story of Alaska's Flag. Primary Grade Activities.

    ERIC Educational Resources Information Center

    Alaska State Museum, Juneau.

    This activities booklet focuses on the story of Alaska's flag. The booklet is intended for teachers to use with primary-grade children. Each activity in the booklet contains background information, a summary and time estimate, Alaska state standards, a step-by-step technique for implementing the activity, assessment tips, materials and resource…

  4. Phloem Transport of Arsenic Species from Flag Leaf to Grain During Grain Filling

    SciTech Connect

    A Carey; G Norton; C Deacon; K Scheckel; E Lombi; T Punshon; M Guerinot; A Lanzirotti; M Newville; et al.

    2011-12-31

    Strategies to reduce arsenic (As) in rice grain, below concentrations that represent a serious human health concern, require that the mechanisms of As accumulation within grain be established. Therefore, retranslocation of As species from flag leaves into filling rice grain was investigated. Arsenic species were delivered through cut flag leaves during grain fill. Spatial unloading within grains was investigated using synchrotron X-ray fluorescence (SXRF) microtomography. Additionally, the effect of germanic acid (a silicic acid analog) on grain As accumulation in arsenite-treated panicles was examined. Dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) were extremely efficiently retranslocated from flag leaves to rice grain; arsenate was poorly retranslocated, and was rapidly reduced to arsenite within flag leaves; arsenite displayed no retranslocation. Within grains, DMA rapidly dispersed while MMA and inorganic As remained close to the entry point. Germanic acid addition did not affect grain As in arsenite-treated panicles. Three-dimensional SXRF microtomography gave further information on arsenite localization in the ovular vascular trace (OVT) of rice grains. These results demonstrate that inorganic As is poorly remobilized, while organic species are readily remobilized, from leaves to grain. Stem translocation of inorganic As may not rely solely on silicic acid transporters.

  5. Phloem transport of arsenic species from flag leaf to grain during grain filling

    SciTech Connect

    Carey, Anne-Marie; Norton, Gareth J.; Deacon, Claire; Scheckel, Kirk G.; Lombi, Enzo; Punshon, Tracy; Guerinot, Mary Lou; Lanzirotti, Antonio; Newville, Matt; Choi, Yongseong; Price, Adam H.; Meharg, Andrew A.

    2011-09-20

    Strategies to reduce arsenic (As) in rice grain, below concentrations that represent a serious human health concern, require that the mechanisms of As accumulation within grain be established. Therefore, retranslocation of As species from flag leaves into filling rice grain was investigated. Arsenic species were delivered through cut flag leaves during grain fill. Spatial unloading within grains was investigated using synchrotron X-ray fluorescence (SXRF) microtomography. Additionally, the effect of germanic acid (a silicic acid analog) on grain As accumulation in arsenite-treated panicles was examined. Dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) were extremely efficiently retranslocated from flag leaves to rice grain; arsenate was poorly retranslocated, and was rapidly reduced to arsenite within flag leaves; arsenite displayed no retranslocation. Within grains, DMA rapidly dispersed while MMA and inorganic As remained close to the entry point. Germanic acid addition did not affect grain As in arsenite-treated panicles. Three-dimensional SXRF microtomography gave further information on arsenite localization in the ovular vascular trace (OVT) of rice grains. These results demonstrate that inorganic As is poorly remobilized, while organic species are readily remobilized, from leaves to grain. Stem translocation of inorganic As may not rely solely on silicic acid transporters.

  6. 14 CFR 121.607 - Communication and navigation facilities: Domestic and flag operations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and..., no person may dispatch an airplane over an approved route or route segment unless the communication... holder may dispatch an airplane over that route or route segment if the pilot in command and...

  7. 14 CFR 121.607 - Communication and navigation facilities: Domestic and flag operations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and..., no person may dispatch an airplane over an approved route or route segment unless the communication... holder may dispatch an airplane over that route or route segment if the pilot in command and...

  8. 14 CFR 121.607 - Communication and navigation facilities: Domestic and flag operations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and..., no person may dispatch an airplane over an approved route or route segment unless the communication... holder may dispatch an airplane over that route or route segment if the pilot in command and...

  9. 14 CFR 121.607 - Communication and navigation facilities: Domestic and flag operations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and..., no person may dispatch an airplane over an approved route or route segment unless the communication... holder may dispatch an airplane over that route or route segment if the pilot in command and...

  10. 14 CFR 121.607 - Communication and navigation facilities: Domestic and flag operations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AND OPERATIONS OPERATING REQUIREMENTS: DOMESTIC, FLAG, AND SUPPLEMENTAL OPERATIONS Dispatching and..., no person may dispatch an airplane over an approved route or route segment unless the communication... holder may dispatch an airplane over that route or route segment if the pilot in command and...

  11. 40 CFR 158.34 - Flagging of studies for potential adverse effects.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... Table—Flagging Criteria Study Type(s) Guideline No. Criteria: Treated animals show any of the following... at any dose level, compared to concurrent control animals of the same sex; or 2 An increase in any type of uncommon or rare neoplasms in any test group, males or females animals at any dose level...

  12. 40 CFR 158.34 - Flagging of studies for potential adverse effects.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... Table—Flagging Criteria Study Type(s) Guideline No. Criteria: Treated animals show any of the following... at any dose level, compared to concurrent control animals of the same sex; or 2 An increase in any type of uncommon or rare neoplasms in any test group, males or females animals at any dose level...

  13. 76 FR 39885 - Risk-Based Targeting of Foreign Flagged Mobile Offshore Drilling Units (MODUs)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

    ... SECURITY Coast Guard Risk-Based Targeting of Foreign Flagged Mobile Offshore Drilling Units (MODUs) AGENCY... Drilling Units (MODUs). This policy letter announces changes to the Coast Guard's system used to prioritize... drilling unit (MODU) must undergo a Coast Guard Certificate of Compliance (COC) examination in order...

  14. 36 CFR § 520.10 - Placards, signs, banners, and flags.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Placards, signs, banners, and flags. § 520.10 Section § 520.10 Parks, Forests, and Public Property SMITHSONIAN INSTITUTION RULES AND REGULATIONS GOVERNING THE BUILDINGS AND GROUNDS OF THE NATIONAL ZOOLOGICAL PARK OF THE SMITHSONIAN INSTITUTION...

  15. Re-induction with L-DNR/FLAG improves response after AML relapse, but not long-term survival.

    PubMed

    Creutzig, U; Semmler, J; Kaspers, G L; Reinhardt, D; Zimmermann, M

    2014-11-01

    According to the results of the international study Relapsed AML 2001/01 response was better after re-induction with L-DNR/FLAG (liposomal daunorubicin, fludarabine, cytarabine, G-CSF) compared to FLAG only but survival rate was not improved. However, the findings might be group-specific. Patient characteristics, actual therapy given and long-term course of the disease in 155 pediatric patients (including non-randomized) with first relapse and 10 primary nonresponders treated in Germany were analyzed. Overall 4-year survival rates after relapse were similar in the 2 treatment groups L-DNR/FLAG and FLAG (0.43 ± 0.05 vs. 0.47 ± 0.06, p(log-rank)=0.47). The rate of randomization was low (65%) and 5% of the 101 randomized patients changed the treatment arm. Therefore, induction was based in 40% patients on an individual decision with preference for L-DNR/FLAG. There were less patients with favorable cytogenetics and morphology in the L-DNR/FLAG-group (p<0.04). Response to the first re-induction course at day 28 tended to be more unfavorable with FLAG only. In this patient group protocol intensifications were more frequent as compared to the L-DNR/FLAG-group (p=0.07), and late CR could be achieved after intensification in 9/18 poor responding patients. The initial selection bias of relapse patients with unfavorable risk factors to the disadvantage of the L-DNR/FLAG-group and the more drug- and time-intensive treatment after 1(st) re-induction given in the FLAG-group may have nullified the initial beneficial effect of L-DNR containing re-induction therapy and led to similar and relatively favorable survival rates in both treatment groups in Germany. © Georg Thieme Verlag KG Stuttgart · New York.

  16. Red eyes and red-flags: improving ophthalmic assessment and referral in primary care.

    PubMed

    Kilduff, Caroline; Lois, Charis

    2016-01-01

    Up to five percent of primary care consultations are eye-related, yet 96% of General Practitioners (GPs) do not undergo postgraduate ophthalmology training. Most do not feel assured performing eye assessments. Some red eye conditions can become sight threatening, and often exhibit red-flag features. These features include moderate pain, photophobia, reduced visual acuity (VA), eye-trauma, or unilateral marked redness. The aim of this project was to improve primary care assessment and referral of patients presenting with red-flag features based on the NICE 'Red Eye' Clinical Knowledge Summary recommendations. Data was collected retrospectively from 139 red eye consultations. A practice meeting highlighted poor awareness of red-flag features, low confidence levels in eye assessments, and time-constraints during appointments. Interventions were based on feedback from staff. These included a primary care teaching session on red-flag features, a VA measurement tutorial, and provision of a red eye toolkit, including VA equipment, to each consultation room. At baseline, each patient had on average 0.9 red-flag features assessed. Only 36.0% (9/25) of patients with red-flag features were appropriately referred to same-day ophthalmology services. Following two improvement cycles, a significant improvement was seen in almost every parameter. On average, each patient had 2.7 red-flag features assessed (vs 0.9, p<0.001). VA was assessed in 55.6% of consultations (vs 7.9%, p<0.001), pain was quantified in 81.5% (vs 20.9%, p=0.005), eye-trauma or foreign-body (51.8% vs 8.6%, p<0.001), extent of redness was documented in 66.7% (vs 14.4%, p<0.001). Only photophobia remained poorly assessed (18.5% vs 14.4%, p=0.75). Following this, 75.0% (6/8) of patients were appropriately referred. This project reflected the literature regarding low confidence and inexperience amongst GPs when faced with ophthalmic conditions. Improvements in education are required to ensure accurate assessments

  17. Red eyes and red-flags: improving ophthalmic assessment and referral in primary care

    PubMed Central

    Kilduff, Caroline; Lois, Charis

    2016-01-01

    Up to five percent of primary care consultations are eye-related, yet 96% of General Practitioners (GPs) do not undergo postgraduate ophthalmology training. Most do not feel assured performing eye assessments. Some red eye conditions can become sight threatening, and often exhibit red-flag features. These features include moderate pain, photophobia, reduced visual acuity (VA), eye-trauma, or unilateral marked redness. The aim of this project was to improve primary care assessment and referral of patients presenting with red-flag features based on the NICE ‘Red Eye’ Clinical Knowledge Summary recommendations. Data was collected retrospectively from 139 red eye consultations. A practice meeting highlighted poor awareness of red-flag features, low confidence levels in eye assessments, and time-constraints during appointments. Interventions were based on feedback from staff. These included a primary care teaching session on red-flag features, a VA measurement tutorial, and provision of a red eye toolkit, including VA equipment, to each consultation room. At baseline, each patient had on average 0.9 red-flag features assessed. Only 36.0% (9/25) of patients with red-flag features were appropriately referred to same-day ophthalmology services. Following two improvement cycles, a significant improvement was seen in almost every parameter. On average, each patient had 2.7 red-flag features assessed (vs 0.9, p<0.001). VA was assessed in 55.6% of consultations (vs 7.9%, p<0.001), pain was quantified in 81.5% (vs 20.9%, p=0.005), eye-trauma or foreign-body (51.8% vs 8.6%, p<0.001), extent of redness was documented in 66.7% (vs 14.4%, p<0.001). Only photophobia remained poorly assessed (18.5% vs 14.4%, p=0.75). Following this, 75.0% (6/8) of patients were appropriately referred. This project reflected the literature regarding low confidence and inexperience amongst GPs when faced with ophthalmic conditions. Improvements in education are required to ensure accurate

  18. Potential synergy between the Ionospheric Disturbance Flag and NeQuick-G for single frequency users

    NASA Astrophysics Data System (ADS)

    Aragon-Angel, Angela; Fortuny, Joaquim

    2016-04-01

    The document describing the particular ionospheric model developed for the Galileo satellite navigation system has been very recently released, the official Ionospheric Correction Algorithm for Galileo Single Frequency Users document (from here on-wards named Galileo-Iono), available at www.gsc-europa.eu/system/files/galileo_documents/Galileo_Ionospheric_Model.pdf. This publication allows GNSS receiver manufacturers to start the implementation of the specific algorithm targeted for their Galileo related products in order to be compliant with the Galileo system. As indicated in the Galileo OS SIS ICD, among the parameters that are broadcast in the Galileo navigation message, parameters that are sent within both F/NAV and I/NAV, one can find five Ionospheric Disturbance Flags for Regions 1 to 5 (SF1, SF2, SF3, SF4 and SF5). Nevertheless, in the current version of the model presented in the Galileo-Iono document, the Ionospheric Disturbance flags are "not used" within the Galileo ionospheric correction calculation. In this work, a potential approach to account for this information is being investigated. This plan includes the update of the Galileo ionospheric, NeQuick-G, correction model by specifying the use of these flags. Hence a customized version of the NeQuick-G model has been developed and tested. Specific scenarios will be considered to test whether this approach of considering the added value information of the Ionospheric Disturbance Flags is translated into the positioning domain. In order to assess the improvement obtained using the proposed approach five stations displaced in the five regions are used. Different days of data have been collected in nominal and disturbed conditions; the evaluation is carried out comparing the performance of the proposed approach with respect to the classical approach. The benefits of the use of the disturbance flags information are evaluated comparing the performance in similar geometry conditions.

  19. 7 CFR 2.8 - Delegations of authority to agency heads to order that the United States flag be flown at half...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... the United States flag be flown at half-staff. 2.8 Section 2.8 Agriculture Office of the Secretary of... heads to order that the United States flag be flown at half-staff. Pursuant to section 5 of Proclamation... order that the United States flag shall be flown at half-staff on buildings and grounds under his or...

  20. 7 CFR 2.8 - Delegations of authority to agency heads to order that the United States flag be flown at half...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... the United States flag be flown at half-staff. 2.8 Section 2.8 Agriculture Office of the Secretary of... heads to order that the United States flag be flown at half-staff. Pursuant to section 5 of Proclamation... order that the United States flag shall be flown at half-staff on buildings and grounds under his or...

  1. 7 CFR 2.8 - Delegations of authority to agency heads to order that the United States flag be flown at half...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... the United States flag be flown at half-staff. 2.8 Section 2.8 Agriculture Office of the Secretary of... heads to order that the United States flag be flown at half-staff. Pursuant to section 5 of Proclamation... order that the United States flag shall be flown at half-staff on buildings and grounds under his or...

  2. 76 FR 79764 - Use of Foreign-Flag Anchor Handling Vessels in the Beaufort Sea or Chukchi Sea Adjacent to Alaska

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ... Maritime Administration Use of Foreign-Flag Anchor Handling Vessels in the Beaufort Sea or Chukchi Sea...-flag anchor handling vessels in certain cases (and for a limited period of time) if no U.S.-flag... anchor handling vessels with a minimum ice class A3 has been received by the Maritime Administration. If...

  3. A Complete System for Polyps Flagging in Virtual Colonoscopy

    DTIC Science & Technology

    2011-04-01

    in this paper) and curvedness as geometric features, applying fuzzy clustering and then using directional gradient concentration to reduce false...with a relative low false positives ( FP ) rate, using heuristic thresholds and texture features. Hong et al., [18], map the 3D surface to a rectangle...polyps submerged in fluid in the database, the results are very promising, achieving 96.4% sensitivity (over 28 polyps) with 1.1 FP per case. However

  4. Field evidence of social influence in the expression of political preferences: the case of secessionists flags in Barcelona.

    PubMed

    Parravano, Antonio; Noguera, José A; Hermida, Paula; Tena-Sánchez, Jordi

    2015-01-01

    Models of social influence have explored the dynamics of social contagion, imitation, and diffusion of different types of traits, opinions, and conducts. However, few behavioral data indicating social influence dynamics have been obtained from direct observation in "natural" social contexts. The present research provides that kind of evidence in the case of the public expression of political preferences in the city of Barcelona, where thousands of citizens supporting the secession of Catalonia from Spain have placed a Catalan flag in their balconies and windows. Here we present two different studies. 1) During July 2013 we registered the number of flags in 26% of the electoral districts in the city of Barcelona. We find that there is a large dispersion in the density of flags in districts with similar density of pro-independence voters. However, by comparing the moving average to the global mean we find that the density of flags tends to be fostered in electoral districts where there is a clear majority of pro-independence vote, while it is inhibited in the opposite cases. We also show that the distribution of flags in the observed districts deviates significantly from that of an equivalent random distribution. 2) During 17 days around Catalonia's 2013 national holiday we observed the position at balcony resolution of the flags displayed in the facades of a sub-sample of 82 blocks. We compare the 'clustering index' of flags on the facades observed each day to thousands of equivalent random distributions. Again we provide evidence that successive hangings of flags are not independent events but that a local influence mechanism is favoring their clustering. We also find that except for the national holiday day the density of flags tends to be fostered in facades located in electoral districts where there is a clear majority of pro-independence vote.

  5. Field Evidence of Social Influence in the Expression of Political Preferences: The Case of Secessionists Flags in Barcelona

    PubMed Central

    Parravano, Antonio; Noguera, José A.; Hermida, Paula; Tena-Sánchez, Jordi

    2015-01-01

    Models of social influence have explored the dynamics of social contagion, imitation, and diffusion of different types of traits, opinions, and conducts. However, few behavioral data indicating social influence dynamics have been obtained from direct observation in “natural” social contexts. The present research provides that kind of evidence in the case of the public expression of political preferences in the city of Barcelona, where thousands of citizens supporting the secession of Catalonia from Spain have placed a Catalan flag in their balconies and windows. Here we present two different studies. 1) During July 2013 we registered the number of flags in 26% of the electoral districts in the city of Barcelona. We find that there is a large dispersion in the density of flags in districts with similar density of pro-independence voters. However, by comparing the moving average to the global mean we find that the density of flags tends to be fostered in electoral districts where there is a clear majority of pro-independence vote, while it is inhibited in the opposite cases. We also show that the distribution of flags in the observed districts deviates significantly from that of an equivalent random distribution. 2) During 17 days around Catalonia’s 2013 national holiday we observed the position at balcony resolution of the flags displayed in the facades of a sub-sample of 82 blocks. We compare the ‘clustering index’ of flags on the facades observed each day to thousands of equivalent random distributions. Again we provide evidence that successive hangings of flags are not independent events but that a local influence mechanism is favoring their clustering. We also find that except for the national holiday day the density of flags tends to be fostered in facades located in electoral districts where there is a clear majority of pro-independence vote. PMID:25961562

  6. Kinematics of the Typical Beach Flags Start for Young Adult Sprinters

    PubMed Central

    Lockie, Robert G.; Vickery, William M.; de Jonge, Xanne A.K. Janse

    2012-01-01

    This study profiled beach flags start kinematics for experienced young adult sprinters. Five males and three females (age = 20.8 ± 2.1 years; height = 1.70 ± 0.06 meters [m]; mass = 63.9 ± 6.0 kilograms) completed four sprints using their competition start technique. A high-speed camera, positioned laterally, filmed the start. Data included: start time; hand clearance time; posterior movement from the start line; feet spacing during the start; elbow, hip, knee, trunk lean, and trajectory angles at take-off; and first step length. Timing gates recorded 0-2, 0-5, and 0-20 m time. Spearman’s correlations identified variables relating (p ≤ 0.05) to faster start and sprint times. The beach flags start involved sprinters moving 0.18 ± 0.05 m posterior to the start line by flexing both legs underneath the body before turning. Following the turn, the feet were positioned 0.47 ± 0.07 apart. This distance negatively correlated with start (ρ = -0.647), 0-2 (ρ = -0.683), and 0-5 m (ρ = -0.766) time. Beach flags start kinematics at take-off resembled research analyzing track starts and acceleration. The elbow extension angle (137.62 ± 13.45°) of the opposite arm to the drive leg correlated with 0-2 (ρ = -0.762), 0-5 (ρ = -0.810), and 0-20 m (ρ = -0.810) time. Greater arm extension likely assisted with stability during the start, leading to enhanced sprint performance. The drive leg knee extension angle (146.36 ± 2.26°) correlated with start time (ρ = -0.677), indicating a contribution to a faster start completion. A longer first step following the start related to faster 0-5 m time (ρ = -0.690). Sprinters quicker over 0-2 and 0-5 m were also quicker over 20 m (ρ = 0.881-0.952). Beach flags sprinters must ensure their start is completed quickly, such that they can attain a high speed throughout the race. Key pointsThere are specific movement patterns adopted by beach flags sprinters during the start. Sprinters will move posterior to the start time prior to

  7. Mandating responsible flagging practices as a strategy for reducing the risk of coastal oil spills.

    PubMed

    Miller, Dana D; Hotte, Ngaio; Sumaila, U Rashid

    2014-04-15

    As human civilization is becoming more aware of the negative impact our actions can inflict upon the natural world, the intensification of fossil fuel extraction and industrial development is being met with increasing opposition. In Western Canada, proposals that would increase the volume of petroleum transported by pipelines and by tankers through the coastal waters of British Columbia have engaged the province in debate. To ease public concern on the risk of a coastal oil spill, there are additional commitments that involved parties could make. There is evidence to show that the practice of registering vessels under foreign flags of states that have exhibited failure in compliance with international obligations is more common amongst petroleum tankers that have been involved in large-scale oil spills. To prove that they are committed to reducing the risk of oil spills, businesses need to stop registering their vessels under flags of foreign, non-compliant states.

  8. Kinematics of the typical beach flags start for young adult sprinters.

    PubMed

    Lockie, Robert G; Vickery, William M; Janse de Jonge, Xanne A K

    2012-01-01

    This study profiled beach flags start kinematics for experienced young adult sprinters. Five males and three females (age = 20.8 ± 2.1 years; height = 1.70 ± 0.06 meters [m]; mass = 63.9 ± 6.0 kilograms) completed four sprints using their competition start technique. A high-speed camera, positioned laterally, filmed the start. Data included: start time; hand clearance time; posterior movement from the start line; feet spacing during the start; elbow, hip, knee, trunk lean, and trajectory angles at take-off; and first step length. Timing gates recorded 0-2, 0-5, and 0-20 m time. Spearman's correlations identified variables relating (p ≤ 0.05) to faster start and sprint times. The beach flags start involved sprinters moving 0.18 ± 0.05 m posterior to the start line by flexing both legs underneath the body before turning. Following the turn, the feet were positioned 0.47 ± 0.07 apart. This distance negatively correlated with start (ρ = -0.647), 0-2 (ρ = -0.683), and 0-5 m (ρ = -0.766) time. Beach flags start kinematics at take-off resembled research analyzing track starts and acceleration. The elbow extension angle (137.62 ± 13.45°) of the opposite arm to the drive leg correlated with 0-2 (ρ = -0.762), 0-5 (ρ = -0.810), and 0-20 m (ρ = -0.810) time. Greater arm extension likely assisted with stability during the start, leading to enhanced sprint performance. The drive leg knee extension angle (146.36 ± 2.26°) correlated with start time (ρ = -0.677), indicating a contribution to a faster start completion. A longer first step following the start related to faster 0-5 m time (ρ = -0.690). Sprinters quicker over 0-2 and 0-5 m were also quicker over 20 m (ρ = 0.881-0.952). Beach flags sprinters must ensure their start is completed quickly, such that they can attain a high speed throughout the race. Key pointsThere are specific movement patterns adopted by beach flags sprinters during the start. Sprinters will move posterior to the start time prior to

  9. Configurations of an Articulated Arm and Singularities of Special Multi-Flags

    NASA Astrophysics Data System (ADS)

    Pelletier, Fernand; Slayman, Mayada

    2014-06-01

    P. Mormul has classified the singularities of special multi-flags in terms of "EKR class" encoded by sequences j_1,dots, j_k of integers (see [Singularity Theory Seminar, Warsaw University of Technology, Vol. 8, 2003, 87-100] and [Banach Center Publ., Vol. 65, Polish Acad. Sci., Warsaw, 2004, 157-178]). However, A.L. Castro and R. Montgomery have proposed in I>Israel J. Math. 192 (2012), 381-427] a codification of singularities of multi-flags by RC and RVT codes. The main results of this paper describe a decomposition of each ''EKR'' set of depth 1 in terms of RVT codes as well as characterize such a set in terms of configurations of an articulated arm. Indeed, an analogue description for some ''EKR'' sets of depth 2 is provided. All these results give rise to a complete characterization of all ''EKR'' sets for 1≤ k≤ 4.

  10. Environmental Assessment for the Joint Red Flag 󈧉 ADA Activities Nellis Air Force Base

    DTIC Science & Technology

    2005-03-01

    Prescribed by ANSI Std Z39-18 DECISION RECORD AND FINDING OF NO SIGNIFICANT IMPACT (DR/FONSI) JOINT RED FLAG –’05 ADA ACTIVITIES 1 Decision: I have...1500–1508]); Department of the Army Environmental Analysis of Army Actions (32 CFR 651); Department of the Air Force Environmental Impact Analysis...Standard Operating Procedures (SOP’s) and monitoring requirements to minimize potential environmental impacts . The project will be constructed under the

  11. U.S. BICENTENNIAL EXPOSITION AMERICAN FLAG & ARBA SYMBOL PAINTING FINISHED ON THE VAB

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The massive Vehicle Assembly Building provides the focal point for the Bicentennial Exposition on Science and Technology, 3rd Century America. The largest flag ever painted and a large American Revolution Bicentennial Administration symbol adorn the side of the building, overlooking the bicentennial site which features 15 domes housing exhibits. Sixteen federal agencies, 10 U. W. industries and numerous colleges and universities are participating in the Exposition, which runs through September 7.

  12. Genetic dissection of flag leaf morphology in wheat (Triticum aestivum L.) under diverse water regimes.

    PubMed

    Yang, Delong; Liu, Yuan; Cheng, Hongbo; Chang, Lei; Chen, Jingjing; Chai, Shouxi; Li, Mengfei

    2016-06-28

    Morphological traits related to flag leaves are determinant traits influencing plant architecture and yield potential in wheat (Triticum aestivum L.). However, little is known regarding their genetic controls under drought stress. One hundred and twenty F8-derived recombinant inbred lines from a cross between two common wheat cultivars Longjian 19 and Q9086 were developed to identify quantitative trait loci (QTLs) and to dissect the genetic bases underlying flag leaf width, length, area, length to width ratio and basal angle under drought stress and well-watered conditions consistent over four environments. A total of 55 additive and 51 pairs of epistatic QTLs were identified on all 21 chromosomes except 6D, among which additive loci were highly concentrated in a few of same or adjacent marker intervals in individual chromosomes. Two specific marker intervals of Xwmc694-Xwmc156 on chromosome 1B and Xbarc1072-Xwmc272 on chromosome 2B were co-located by additive QTLs for four tested traits. Twenty additive loci were repeatedly detected in more than two environments, suggestive of stable A-QTLs. A majority of QTLs involved significant additive and epistatic effects, as well as QTL × environment interactions (QEIs). Of these, 72.7 % of additive QEIs and 80 % of epistatic QEIs were related to drought stress with significant genetic effects decreasing phenotypic values. By contrast, additive and QEIs effects contributed more phenotypic variation than epistatic effects. Flag leaf morphology in wheat was predominantly controlled by additive and QEIs effects, where more QEIs effects occurred in drought stress and depressed phenotypic performances. Several QTL clusters indicated tight linkage or pleiotropy in the inheritance of these traits. Twenty stable QTLs for flag leaf morphology are potentially useful for the genetic improvement of drought tolerance in wheat through QTL pyramiding.

  13. FLAG: A multi-dimensional adaptive free-Lagrange code for fully unstructured grids

    SciTech Connect

    Burton, D.E.; Miller, D.S.; Palmer, T.

    1995-07-01

    The authors describe FLAG, a 3D adaptive free-Lagrange method for unstructured grids. The grid elements were 3D polygons, which move with the flow, and are refined or reconnected as necessary to achieve uniform accuracy. The authors stressed that they were able to construct a 3D hydro version of this code in 3 months, using an object-oriented FORTRAN approach.

  14. View of the U.S. flag near the Apollo 14 landing site during liftoff

    NASA Image and Video Library

    1971-02-05

    S71-19500 (6 Feb. 1971) --- The Apollo 14 Lunar Module (LM) ascent stage lifts off the lunar surface and the powerful LM engine causes a brief force of wind which scatters gold-colored foil, covering the LM, and disturbs the U.S. flag. This picture was taken from film exposed by the 16mm data acquisition camera - which was mounted inside the LM.

  15. Astronaut John Young leaps from lunar surface as he salutes U.S. flag

    NASA Technical Reports Server (NTRS)

    1972-01-01

    Astronaut John W. Young, commander of the Apollo 16 lunar landing mission, leaps from the lunar surface as he salutes the U.S. flag during the first Apollo 16 extravehicular activity (EVA-1) on the Moon, as seen in this reproduction taken from a color transmission made by the color TV camera mounted on the Lunar Roving Vehicle. Astronaut Charles M. Duke Jr., lunar module pilot, is standing in the background.

  16. Environmental Assessment: Improvements to Silver Flag Training Area at Tyndall Air Force Base, Florida

    DTIC Science & Technology

    2013-01-01

    The Proposed Action involves various improvements to the Silver Flag Training Area at Tyndall AFB. The following seven projects are proposed under... roof and no side walls. The structure would provide covered parking for 16 MRAP vehicles. 2.1.6 Construct Latrine/Shower/Laundry Facility...metal roof . 2.1.7 Convert Spray Field to Bed-Down Training Site This project would involve converting the existing inactive domestic wastewater

  17. An analogue of Weyl’s law for quantized irreducible generalized flag manifolds

    SciTech Connect

    Matassa, Marco E-mail: mmatassa@math.uio.no

    2015-09-15

    We prove an analogue of Weyl’s law for quantized irreducible generalized flag manifolds. This is formulated in terms of a zeta function which, similarly to the classical setting, satisfies the following two properties: as a functional on the quantized algebra it is proportional to the Haar state and its first singularity coincides with the classical dimension. The relevant formulas are given for the more general case of compact quantum groups.

  18. Developing an early alert system for metastatic spinal cord compression (MSCC): Red Flag credit cards.

    PubMed

    Turnpenney, Jackie; Greenhalgh, Sue; Richards, Lena; Crabtree, Annamaria; Selfe, James

    2015-01-01

    To produce a user-friendly list of metastatic spinal cord compression (MSCC) Red Flags for non-specialist 'generalist' front-line clinicians working in primary-care settings. The issue of identifying MSCC early to prevent serious long-term disability was a key theme identified by the Task and Finish Group at Greater Manchester and Cheshire Cancer Network (GMCCN) in 2009. It was this group who initially brokered and then coordinated the current development as part of their strategic approach to improving care for MSCC patients. A consensus-building approach that considered the essential minimum data requirements to raise the index of suspicion suggestive of MSCC was adopted. This followed a model of cross-boundary working to facilitate the mutual sharing of expertise across a variety of relevant clinical specialisms. A guideline aimed at helping clinicians to identify the early signs and symptoms of MSCC was produced in the form of a credit card. This credit card includes key statements about MSCC, signposting to key sources of additional information and a user-friendly list of Red Flags which has been developed into an eight-item Red Flag mnemonic. To date, an excess of 120,000 cards have been printed by a variety of organisations and the distribution of the cards is ongoing across the United Kingdom and the Republic of Ireland.

  19. Red Flags of Organic Recurrent Abdominal Pain in Children: Study on 100 Subjects

    PubMed Central

    Motamed, Farzaneh; Mohsenipour, Reihaneh; Seifirad, Soroush; Yusefi, Azizolah; Farahmand, Fatemeh; Khodadad, Ahmad; Falahi, Gholamhosein; Najafi, Mehri

    2012-01-01

    Objective A variety of sign, symptoms and laboratory findings are more common in children with organic abdominal pains. This study was performed to evaluate the prevalence of organic and functional abdominal pains and relation of red flags to organic pains in 100 children with recurrent abdominal pain (RAP). Methods One hundred consecutive patients with RAP were enrolled in the study. A complete interview and physical examination was made for each patient, accompanied by a series of laboratory, clinical and para-clinical examinations. The data were recorded and analyzed. Logistic regression analysis was used to model and formulize correlations between sign, symptoms, and laboratory findings with organic and functional abdominal pain. Findings Among 100 patients (52% male, 48% female, Age: 9.29±3.17) diagnostic works up revealed organic pain for 57 patients. The most common symptoms of the patients included constipation, diarrhea, chest pain, cough, headache, vomiting, hematuria, and dysuria. Fecal incontinence, delayed puberty, organomegaly, jaundice, and family history of inflammatory bowel disease were reported in none of the patients with RAP. Fever, pain not located in periumbilical area, nocturnal pain, elevated erythrocyte sedimentation rate, weight loss, growth disorder, and abdominal tenderness were among the red flags which revealed diagnosis of organic pain in this study. Conclusion A series of red flags could increase likelihood of finding organic pain in children with RAP. PMID:23429658

  20. Implementation and Validation of the BHR Turbulence Model in the FLAG Hydrocode

    SciTech Connect

    Denissen, Nicholas A.; Fung, Jimmy; Reisner, Jon M.; Andrews, Malcolm J.

    2012-08-29

    The BHR-2 turbulence model, developed at Los Alamos National Laboratory for variable density and compressible flows, is implemented in an Arbitrary Lagrangian-Eulerian hydrocode, FLAG. The BHR-2 formulation is discussed, with emphasis on its connection to multi-component flow formulations that underlie FLAG's treatment of multi-species flow. One-dimensional and two-dimensional validation tests are performed and compared to experiment and Eulerian simulations. Turbulence is an often studied and ubiquitous phenomenon in nature, and modeling its effects is essential in many practical applications. Specifically the behavior of turbulence in the presence of strong density gradients and compressibility is of fundamental importance in applications ranging from Inertial Confinement Fusion (ICF) [1], supernovae [2], and atmospheric flows. The BHR closure approach [3] seeks to model the physical processes at work in variable density turbulence including Kelvin-Helmholtz (KH) [4], Rayleigh-Taylor (RT) [5], and Richtmyer-Meshkov (RM) [6], driven turbulence. The effectiveness of the BHR-2 implementation has been demonstrated for variable density mixing in the KH, RT, and RM cases in an Eulerian framework [7]. The primary motivation of the present work is to implement the BHR-2 turbulence model in the Arbitrary Lagrangian-Eulerian (ALE) hydrodynamics code FLAG. The goal is not only to demonstrate results in agreement with previous Eulerian calculations, but also document behavior that arises from the underlying differences in code philosophy.

  1. Composition of cuticular waxes coating flag leaf blades and peduncles of Triticum aestivum cv. Bethlehem.

    PubMed

    Racovita, Radu C; Hen-Avivi, Shelly; Fernandez-Moreno, Josefina-Patricia; Granell, Antonio; Aharoni, Asaph; Jetter, Reinhard

    2016-10-01

    The work herein presents comprehensive analyses of the cuticular wax mixtures covering the flag leaf blade and peduncle of bread wheat (Triticum aestivum) cv. Bethlehem. Overall, Gas Chromatography-Mass Spectrometry and Flame Ionization Detection revealed a wax coverage of flag leaf blades (16 μg/cm(2)) a third that of peduncles (49 μg/cm(2)). Flag leaf blade wax was dominated by 1-alkanols, while peduncle wax contained primarily β-diketone and hydroxy-β-diketones, thus suggesting differential regulation of the acyl reduction and β-diketone biosynthetic pathways in the two analyzed organs. The characteristic chain length distributions of the various wax compound classes are discussed in light of their individual biosynthetic pathways and biosynthetic relationships between classes. Along with previously reported wheat wax compound classes (fatty acids, 1-alkanols, 1-alkanol esters, aldehydes, alkanes, β-diketone, hydroxy-β-diketones, alkylresorcinols and methyl alkylresorcinols), esters of 2-alkanols and three types of aromatic esters (benzyl, phenethyl and p-hydroxyphenethyl) are also reported. In particular, 2-heptanol esters were identified. Detailed analyses of the isomer distributions within 1-alkanol and 2-alkanol ester homologs revealed distinct patterns of esterified acids and alcohols, suggesting several wax ester synthases with very different substrate preferences in both wheat organs. Terpenoids, including two terpenoid esters, were present only in peduncle wax.

  2. Expression of FLAG fusion proteins in insect cells: application to the multi-subunit transcription/DNA repair factor TFIIH.

    PubMed

    Jawhari, Anass; Uhring, Muriel; Crucifix, Corinne; Fribourg, Sébastien; Schultz, Patrick; Poterszman, Arnaud; Egly, Jean Marc; Moras, Dino

    2002-04-01

    The multi-subunit transcription/DNA repair factor TFIIH was used as a model system to show that the expression of FLAG fusion proteins in insect cells constitutes a versatile tool for both structural and functional investigations. In the present study, we have constructed recombinant baculoviruses expressing the four core TFIIH subunits fused at their N-terminus to the FLAG peptide. Using these recombinant viruses we have established protocols based on anti-FLAG immunoaffinity chromatography that allow the systematic analysis of pairwise interaction within multiprotein complexes and have developed a double tag strategy (FLAG and hexahistidine tags) for the identification and purification of stable TFIIH subcomplexes. A simple purification procedure was developed that leads to the isolation of recombinant TFIIH containing the full set of subunits. The purified recombinant TFIIH was shown to be active in a transcription assay and to be structurally homologous to the endogenous complex by electron microscopy and image analysis.

  3. World War II soldier salutes the US Flag during a Veteran's Day ceremony to dedicate a memorial

    NASA Technical Reports Server (NTRS)

    2005-01-01

    World War II soldier salutes the US Flag during a Veteran's Day ceremony to dedicate a memorial to 'Smoky, Yorkie Doodle Dandy and Dogs of All Wars' in the Rocky River Reservation, Lakewood, Ohio. November 11, 2005

  4. Red flags to screen for malignancy in patients with low-back pain.

    PubMed

    Henschke, Nicholas; Maher, Christopher G; Ostelo, Raymond W J G; de Vet, Henrica C W; Macaskill, Petra; Irwig, Les

    2013-02-28

    The identification of serious pathologies, such as spinal malignancy, is one of the primary purposes of the clinical assessment of patients with low-back pain (LBP). Clinical guidelines recommend awareness of "red flag" features from the patient's clinical history and physical examination to achieve this. However, there are limited empirical data on the diagnostic accuracy of these features and there remains very little information on how best to use them in clinical practice. To assess the diagnostic performance of clinical characteristics identified by taking a clinical history and conducting a physical examination ("red flags") to screen for spinal malignancy in patients presenting with LBP. We searched electronic databases for primary studies (MEDLINE, EMBASE, and CINAHL) and systematic reviews (PubMed and Medion) from the earliest date until 1 April 2012. Forward and backward citation searching of eligible articles was also performed. We considered studies if they compared the results of history taking and physical examination on patients with LBP with those of diagnostic imaging (magnetic resonance imaging, computed tomography, myelography). Two review authors independently assessed the quality of each included study with the QUality Assessment of Diagnostic Accuracy Studies (QUADAS) tool and extracted details on patient characteristics, study design, index tests, and reference standard. Diagnostic accuracy data were presented as sensitivities and specificities with 95% confidence intervals for all index tests. We included eight cohort studies of which six were performed in primary care (total number of patients; n = 6622), one study was from an accident and emergency setting (n = 482), and one study was from a secondary care setting (n = 257). In the six primary care studies, the prevalence of spinal malignancy ranged from 0% to 0.66%. Overall, data from 20 index tests were extracted and presented, however only seven of these were evaluated by more than one

  5. Evaluation of the automated hematology analyzer Sysmex XT-2000iV ™ compared to the ADVIA ® 2120 for its use in dogs, cats, and horses. Part II: Accuracy of leukocyte differential and reticulocyte count, impact of anticoagulant and sample aging.

    PubMed

    Bauer, Natali; Nakagawa, Julia; Dunker, Cathrin; Failing, Klaus; Moritz, Andreas

    2012-01-01

    The automated laser-based hematology analyzer Sysmex XT-2000iV™ provides a 5-part differential count and specific cytograms that are of great interest for large veterinary laboratories. The aim of the study was to validate the Sysmex XT-2000iV compared to the laser-based hematology analyzer ADVIA® 2120 and manual differential in dogs, cats, and horses as well as the impact of anticoagulant (heparin, ethylenediamine tetra-acetic acid [EDTA], and citrate) and storage at 22°C and 4°C. Consecutive fresh K(3)-EDTA blood samples from 216 cats, 314 dogs, and 174 horses were included. The impact of anticoagulant and sample storage was assessed in specimens obtained from an additional 9 cats, 10 dogs, and 10 horses. Agreement between both analyzers was excellent to good except for monocytes and canine reticulocytes. Spearman rank correlation coefficients (r (s)) between Sysmex XT-2000iV and manual differential were good to fair and ranged from 0.91 (cat lymphocytes) to 0.44 (cat monocytes). Hematocrit value (Hct), mean corpuscular hemoglobin (MCH), MCH concentration (MCHC; all: P < 0.001), and mean corpuscular volume (MCV; P < 0.01) were higher in canine citrated blood compared to heparin and EDTA. In cats, lymphocytes and monocytes were lower in heparinized blood compared to EDTA (P < 0.05), whereas in horses no significant effect was seen. Regarding storage time and temperature, white and red blood cell counts, hemoglobin, and MCH were stable. Hct, MCV, and MCHC were influenced by erythrocyte swelling. Differential count remained stable for 24 hr (22°C) and nearly 72 hr (4°C) except for monocytes. The overall performance of the Sysmex XT-2000iV was excellent and compared favorably with that of the ADVIA 2120. A special strength was the excellent detection of feline eosinophils.

  6. Agatha's Flag

    ERIC Educational Resources Information Center

    Williams, Lee Burdette

    2006-01-01

    For years, educators had complained that college students were apathetic, that their turnout at the polls was pitifully low, that they didn't seem to care about national politics. Despite MTV's Rock the Vote campaign in the 2000 election, students didn't show up to vote in any numbers that gave everybody the reason to be hopeful about the nation's…

  7. Agatha's Flag

    ERIC Educational Resources Information Center

    Williams, Lee Burdette

    2006-01-01

    For years, educators had complained that college students were apathetic, that their turnout at the polls was pitifully low, that they didn't seem to care about national politics. Despite MTV's Rock the Vote campaign in the 2000 election, students didn't show up to vote in any numbers that gave everybody the reason to be hopeful about the nation's…

  8. Dynamic Black-Level Correction and Artifact Flagging for Kepler Pixel Time Series

    NASA Astrophysics Data System (ADS)

    Clarke, Bruce; Kolodziejczak, J. J.; Caldwell, D. A.

    2012-05-01

    Instrument-induced artifacts in the raw Kepler pixel data include time-varying crosstalk from the fine guidance sensor (FGS) clock signals, manifestations of drifting moiré pattern as locally correlated nonstationary noise and rolling bands in the images which find their way into the calibrated pixel time series and ultimately into the calibrated target flux time series. As the Kepler Mission continues to improve the fidelity of its science data products, we are evaluating the benefits of adding pipeline steps to more completely model and dynamically correct the FGS crosstalk, then use the residuals from these model fits to detect and flag spatial regions and time intervals of strong time-varying black-level which may complicate later processing or lead to misinterpretation of instrument behavior as stellar activity. The FGS crosstalk pixels are present in 20-25% of targets but typically vary slowly enough to create a very small risk of reduced sensitivity or increased false positive rate in the transit search. However, they do have the potential to complicate or reduce the effectiveness of cotrending algorithms by introducing additional cotrending terms into the light curves which are not associated with prior relations. We will present results regarding the improvement in cotrending performance as a result of including FGS corrections in the calibration. The rolling bands appear in only 10% of channels and are present only in 3% of the total exposure, but we estimate that, because of the rotation of stars through the affected sky groups, about 30% of light curves are ultimately affected. Thus the utility of rolling band flagging is expected to be high. We will discuss the effectiveness of the proposed flagging and illustrate with some affected light curves. Funding for the Kepler Mission has been provided by the NASA Science Mission Directorate.

  9. Clinical study of Mito-FLAG regimen in treatment of relapsed acute myeloid leukemia.

    PubMed

    Luo, Sheng; Cai, Fangfang; Jiang, Lei; Zhang, Shenghui; Shen, Zhijian; Sun, Lan; Gao, Shenmeng

    2013-03-01

    Patients with relapsed acute myeloid leukemia (AML) have unfavorable prognosis and require innovative therapeutic approaches. In this study we used fludarabine combined with a middle dose of cytosine arabinoside (Ara-C), mitoxantrone and granulocyte-colony stimulating factor (G-CSF) as a salvage therapy for patients with relapsed AML in China. Forty-five patients with relapsed AML were treated with the Mito-FLAG regimen consisting of mitoxantrone (7 mg/m(2), day 1, 3 and 5), fludarabine (30 mg/m(2), days 1-5), Ara-C (1 g/m(2), over 3 h every 12 h, days 1-5) and G-CSF [5 μg/kg/day subcutaneously from day 0 until the white blood count (WBC) was >20×10(9)/l]. Patients with a partial response (PR) received another course of the same regimen. Patients with a suitable donor and aged <50 years received allogeneic stem cell transplantation (allo-SCT). Twenty-three patients (51%) and 3 patients (7%) achieved complete remission (CR) and PR, respectively, following one or two courses of Mito-FLAG, and the overall response (OR) rate was 58%. Nine patients (20%) received allo-SCT and 4 patients (9%) succumbed early. Hematological toxicity and infections were the most prominent toxicities of this regimen. Other toxicities included nausea, vomiting, bleeding, hyperbilirubinemia, renal toxicity and arrhythmia. The probability of overall survival (OS) at 4 years was 19% (95% CI, 11-26%) and the probability of 4-year disease-free survival (DFS) was 29% for all 23 patients in CR (95% CI, 18-41%). Our data suggest that Mito-FLAG is a highly effective and well-tolerated salvage regimen for relapsed AML.

  10. Accessing numeric data via flags and tags: A final report on a real world experiment

    NASA Technical Reports Server (NTRS)

    Kottenstette, J. P.; Freeman, J. E.; Staskin, E. R.; Hargrave, C. W.

    1978-01-01

    An experiment is reported which: extended the concepts of data flagging and tagging to the aerospace scientific and technical literature; generated experience with the assignment of data summaries and data terms by documentation specialists; and obtained real world assessments of data summaries and data terms in information products and services. Inclusion of data summaries and data terms improved users' understanding of referenced documents from a subject perspective as well as from a data perspective; furthermore, a radical shift in document ordering behavior occurred during the experiment toward proportionately more requests for data-summarized items.

  11. U.S. BICENTENNIAL EXPOSITION & PAINTING OF AMERICAN FLAG ON VAB

    NASA Technical Reports Server (NTRS)

    1976-01-01

    Transformation of the U. S. Bicentennial on Science and Technology from an artist's concept to reality is well underway at KSC. At lower right are stages of the Saturn V rocket which will form part of the Exposition. Four of the 15 domes erected to house exhibits by 16 federal agencies and numerous industrial firms are visible in the foreground. At left center, workmen on a scaffold can be seen completing the blue field on the American flag being painted on the Vehicle Assembly Building. The Exposition will be open to the public from May 30 through September 7.

  12. Environmental Assessment Improvements to Silver Flag Training Area at Tyndall Air Force Base, Florida

    DTIC Science & Technology

    2013-08-02

    Installation Compatible Use Zone program, soils , wetlands, smface water, floodplains , vegetation, fish and wildlife, listed species, land use...Pinus elliottii) and   North Source: Tyndall AFB INRMP FIGURE 3-7 100-Year Floodplains at Tyndall AFB EA for Improvements to Silver Flag Training...greater  soil  impacts  than Alternative 1. Appropriate BMPs and erosion/ sedimentation  controls would be implemented during  construction of Alternative 2a

  13. American Flag and Mini-DVD Attached to Deck of Phoenix

    NASA Technical Reports Server (NTRS)

    2008-01-01

    This image, released on Memorial Day, May 26, 2008, shows the American flag and a mini-DVD on the Phoenix's deck, which is about 3 ft. above the Martian surface. The mini-DVD from the Planetary Society contains a message to future Martian explorers, science fiction stories and art inspired by the Red Planet, and the names of more than a quarter million earthlings.

    The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

  14. American Flag and Mini-DVD Attached to Deck of Phoenix

    NASA Technical Reports Server (NTRS)

    2008-01-01

    This image, released on Memorial Day, May 26, 2008, shows the American flag and a mini-DVD on the Phoenix's deck, which is about 3 ft. above the Martian surface. The mini-DVD from the Planetary Society contains a message to future Martian explorers, science fiction stories and art inspired by the Red Planet, and the names of more than a quarter million earthlings.

    The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

  15. Experimental study using Touschek lifetime as machine status flag in SSRF

    NASA Astrophysics Data System (ADS)

    Chen, Zhi-Chu; Leng, Yong-Bin; Yuan, Ren-Xian; Yan, Ying-Bing; Yu, Lu-Yang

    2014-07-01

    The stabilities of the beam and machine have almost the highest priority in a modern light source. Although a lot of machine parameters could be used to represent the beam quality, there is no single parameter that could indicate the global information for the machine operators and accelerator physicists. For the last few years, a new parameter has been studied as a beam quality flag in the Shanghai Synchrotron Radiation Facility (SSRF). Calculations, simulations and detailed analysis of the real-time data from the storage ring have been made and the interesting results have confirmed its feasibility.

  16. U.S. BICENTENNIAL EXPOSITION & PAINTING OF AMERICAN FLAG ON VAB

    NASA Technical Reports Server (NTRS)

    1976-01-01

    Transformation of the U. S. Bicentennial on Science and Technology from an artist's concept to reality is well underway at KSC. At lower right are stages of the Saturn V rocket which will form part of the Exposition. Four of the 15 domes erected to house exhibits by 16 federal agencies and numerous industrial firms are visible in the foreground. At left center, workmen on a scaffold can be seen completing the blue field on the American flag being painted on the Vehicle Assembly Building. The Exposition will be open to the public from May 30 through September 7.

  17. An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments.

    PubMed

    Knappik, A; Plückthun, A

    1994-10-01

    The commercially available monoclonal antibodies M1 and M2 were raised against and bind the FLAG sequence DYKDDDDK with high specificity. Using the calcium-dependent M1 antibody and the FLAG tag attached to the N terminus of various fragments of the antibody McPC603 expressed in Escherichia coli, we found that the M1 antibody binds with almost the same affinity to a much shorter version of this sequence (DYKD). Since most antibody light chains start with an aspartate, the addition of only three additional amino acids to the N terminus is sufficient to detect and quantify the expressed antibody fragments using standard immunological methods. Similarly, the heavy chain can be detected specifically with the sequence DYKD, which requires four additional amino acids since most heavy chains do not start with Asp. The signal sequence of both chains that is necessary for the transport of the chains to the periplasm of E. coli is processed correctly. Furthermore, we investigated the influence of the amino acid at the fifth position of the FLAG sequence on the binding affinity of the M1 antibody and found that a glutamate at this position increased the sensitivity in Western blots sixfold over the original long FLAG sequence containing an aspartate residue at this position. Together, the improved FLAG is a versatile tool for both sensitive detection and one-step purification of recombinant proteins.

  18. Photosynthetic Gas Exchange Characteristics of Wheat Flag Leaf Blades and Sheaths during Grain Filling

    PubMed Central

    Araus, José L.; Tapia, Luis

    1987-01-01

    The rate of net CO2 assimilation (A), the stomatal (gs) and residual (gr) conductances to CO2, the intercellular CO2 concentration, the CO2 compensation points at 21% O2 (Γ21) and at 2% O2 (Γ2), and the amounts of dry matter, nitrogen, and carbohydrates were determined, from anthesis through grain filling, in the flag leaf blade and sheath of spring wheat (Triticum aestivum L. cv Kolibri). The nitrogen content and the rate of net CO2 assimilation declined slowly until the onset of senescence in both organs, about 3 weeks after anthesis. During senescence the reduction of A in both organs was not primarily caused by a decrease in gs; the main factor is the decrease in gr. From values of Γ21 and Γ2 it is suggested that the rate of respiration in the light contributing to the CO2 compensation point is higher in sheaths than in blades irrespective of the O2 level considered. The role of sheaths storing and later transporting assimilates to the developing grains seems to be more important for shoot yield than that of sheaths functioning as photosynthetic organs after the onset of senescence occurs. It is suggested that accumulation of carbohydrates in leaves might somehow trigger senescence in the flag leaf blade and sheath simultaneously. PMID:16665757

  19. The differential diagnosis of Huntington's disease-like syndromes: 'red flags' for the clinician.

    PubMed

    Martino, Davide; Stamelou, Maria; Bhatia, Kailash P

    2013-06-01

    A growing number of progressive heredodegenerative conditions mimic the presentation of Huntington's disease (HD). Differentiating among these HD-like syndromes is necessary when a patient with a combination of movement disorders, cognitive decline, behavioural abnormalities and progressive disease course proves negative to the genetic testing for HD causative mutations, that is, IT15 gene trinucleotide-repeat expansion. The differential diagnosis of HD-like syndromes is complex and may lead to unnecessary and costly investigations. We propose here a guide to this differential diagnosis focusing on a limited number of clinical features ('red flags') that can be identified through accurate clinical examination, collection of historical data and a few routine ancillary investigations. These features include the ethnic background of the patient, the involvement of the facio-bucco-lingual and cervical district by the movement disorder, the co-occurrence of cerebellar features and seizures, the presence of peculiar gait patterns and eye movement abnormalities, and an atypical progression of illness. Additional help may derive from the cognitive-behavioural presentation of the patient, as well as by a restricted number of ancillary investigations, mainly MRI and routine blood tests. These red flags should be constantly updated as the phenotypic characterisation and identification of more reliable diagnostic markers for HD-like syndromes progress over the following years.

  20. Alterations to calling criteria for Between the Flags (an early warning system)

    PubMed Central

    Davis, Tessa; Nogajski, Bec

    2015-01-01

    Early warning systems aim to detect clinical deterioration of patients at an early stage. Between the Flags was introduced in New South Wales Health for this purpose. When patients are transferred from the emergency department to the ward, there are circumstances when the calling criteria need to be altered to take into account the clinical context. It is recognised that confusion exists among junior medical staff about the process of making alterations to the Between the Flags calling criteria. A quality improvement project was implemented by undertaking a baseline survey of junior medical staff, providing education and training (to junior medical staff on the existing guidelines for making alteration to the calling criteria), and conducting a post-implementation survey. A baseline survey demonstrated that 74% of junior medical staff had received no education on making alterations and only 5% knew how long their alterations would last once the patient was transferred to the ward. This has potentially serious consequences for patient safety following transfer. After implementation of training, we found that 63% of junior medical staff were aware of the guidelines on making alterations and 50% knew how long their alterations would last once the patient was transferred to the ward. We conclude that educating junior medical staff improved knowledge on the guidelines for making alterations to calling criteria. PMID:26734326