Consultant management estimating tool : users' manual.

DOT National Transportation Integrated Search

2012-04-01

The Switchboard is the opening form displayed to users. Use : the Switchboard to access the main functions of the estimating : tool. Double-click on a box to select the desired function. From : the Switchboard a user can initiate a search for project...

Efficient Homodifunctional Bimolecular Ring-Closure Method for Cyclic Polymers by Combining RAFT and Self-Accelerating Click Reaction.

PubMed

Qu, Lin; Sun, Peng; Wu, Ying; Zhang, Ke; Liu, Zhengping

2017-08-01

An efficient metal-free homodifunctional bimolecular ring-closure method is developed for the formation of cyclic polymers by combining reversible addition-fragmentation chain transfer (RAFT) polymerization and self-accelerating click reaction. In this approach, α,ω-homodifunctional linear polymers with azide terminals are prepared by RAFT polymerization and postmodification of polymer chain end groups. By virtue of sym-dibenzo-1,5-cyclooctadiene-3,7-diyne (DBA) as small linkers, well-defined cyclic polymers are then prepared using the self-accelerating double strain-promoted azide-alkyne click (DSPAAC) reaction to ring-close the azide end-functionalized homodifunctional linear polymer precursors. Due to the self-accelerating property of DSPAAC ring-closing reaction, this novel method eliminates the requirement of equimolar amounts of telechelic polymers and small linkers in traditional bimolecular ring-closure methods. It facilitates this method to efficiently and conveniently produce varied pure cyclic polymers by employing an excess molar amount of DBA small linkers. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Specialization of the auditory processing in harbor porpoise, characterized by brain-stem potentials

NASA Astrophysics Data System (ADS)

Bibikov, Nikolay G.

2002-05-01

Brain-stem auditory evoked potentials (BAEPs) were recorded from the head surface of the three awaked harbor porpoises (Phocoena phocoena). Silver disk placed on the skin surface above the vertex bone was used as an active electrode. The experiments were performed at the Karadag biological station (the Crimea peninsula). Clicks and tone bursts were used as stimuli. The temporal and frequency selectivity of the auditory system was estimated using the methods of simultaneous and forward masking. An evident minimum of the BAEPs thresholds was observed in the range of 125-135 kHz, where the main spectral component of species-specific echolocation signal is located. In this frequency range the tonal forward masking demonstrated a strong frequency selectivity. Off-response to such tone bursts was a typical observation. An evident BAEP could be recorded up to the frequencies 190-200 kHz, however, outside the acoustical fovea the frequency selectivity was rather poor. Temporal resolution was estimated by measuring BAER recovery functions for double clicks, double tone bursts, and double noise bursts. The half-time of BAERs recovery was in the range of 0.1-0.2 ms. The data indicate that the porpoise auditory system is strongly adapted to detect ultrasonic closely spaced sounds like species-specific locating signals and echoes.

pH responsive label-assisted click chemistry triggered sensitivity amplification for ultrasensitive electrochemical detection of carbohydrate antigen 24-2.

PubMed

Zheng, Yun; Zhao, Lihua; Ma, Zhanfang

2018-05-15

Sensitivity amplification strategy by implementing click chemistry in the construction of biosensing interface can efficiently improve the performance of immunosensor. Herein, we developed a sandwich-type amperometric immunosensor for ultrasensitive detection of carbohydrate antigen 24-2 (CA 242) based on pH responsive label-assisted click chemistry triggered sensitivity amplification strategy. The sensitivity of amperometric immunosensor relies on the current response differences (ΔI) caused by per unit concentration target analyte. The pH responsive Cu 2+ -loaded polydopamine (CuPDA) particles conjugated with detection antibodies were employed as labels, which can release Cu(II) ions by regulating pH. In the presence of ascorbic acid (reductant), Cu(II) ions were reduced to Cu(I) ions. Azide-functionalized double-stranded DNA (dsDNA) as signal enhancer was immobilized on the substrate through Cu + -catalyzed azide/alkyne cycloaddition reaction. With the help of the click reaction, the ΔI caused by target was elevated prominently, resulting in sensitivity amplification of the immunosensor. Under optimal condition, the proposed immunosensor exhibited excellent performance with linear range from 0.0001 to 100 U mL -1 and ultralow detection limit of 20.74 μU mL -1 . This work successfully combines click chemistry with pH-responsive labels in sandwich-type amperometric immunosensor, providing a promising sensitivity amplification strategy to construct immunosensing platform for analysis of other tumor marker. Copyright © 2018 Elsevier B.V. All rights reserved.

CODEM User Manual

DTIC Science & Technology

2010-09-01

If the registration succeeds, at computer restart, “.cdm” files will be associated to a new icon that is the CODEM logo. Double-clicking on such a...should I proceed? A5 : It is suggested that you add your extensions in a separate Jar file. You will then not be able to use the executable file, but

Basic Hearing and Echolocation Mechanisms of Marine Mammals: Measured Auditory Evoked Potential and Behavioral Experiments FY 2007

DTIC Science & Technology

2007-01-01

Breese, M. (2007) Evoked-potential recovery during double click stimulation in a whale: A possibility of biosonar automatic gain control. Journal of...Yokohama Japan (published) Supin A.Ya, Nachtigall, P.E., and Breese, M. Source level to sensation level ratio of transmitted biosonar pulses in an

Selective Cell Adhesion and Biosensing Applications of Bio-Active Block Copolymers Prepared by CuAAC/Thiol-ene Double Click Reactions.

PubMed

Oyman Eyrilmez, Gizem; Doran, Sean; Murtezi, Eljesa; Demir, Bilal; Odaci Demirkol, Dilek; Coskunol, Hakan; Timur, Suna; Yagci, Yusuf

2015-09-01

N-Acetyl-l-cysteine (NAC)-capped poly(methyl methacrylate)-b-polycaprolactone block copolymer (PMMA-b-PCL-NAC) was prepared using the previously described one-pot photoinduced sequential CuAAC/thiol-ene double click procedure. PMMA-b-PCL-NAC had previously shown good applicability as a matrix for cell adhesion of cells from the Vero cell line (African green monkey kidney epithelial). Here, in this work, PMMA-b-PCL-NAC served as an excellent immobilization matrix for biomolecule conjugation. Covalent binding of RGD (R: arginine, G: glycine, and D: aspartic acid) peptide sequence onto the PMMA-b-PCL-NAC-coated surface was performed via EDC chemistry. RGD-modified PMMA-b-PCL-NAC (PMMA-b-PCL-NAC-RGD) as a non-toxic cell proliferation platform was used for selective "integrin αvβ3-mediated cell adhesion and biosensing studies. Both optical and electrochemical techniques were used to monitor the adhesion differences between "integrin αvβ3" receptor positive and negative cell lines on to the designed biofunctional surfaces. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

First results of MAO NASU SS bodies photographic archive digitizing

NASA Astrophysics Data System (ADS)

Pakuliak, L.; Andruk, V.; Shatokhina, S.; Golovnya, V.; Yizhakevych, O.; Kulyk, I.

2013-05-01

MAO NASU glass archive encloses about 1800 photographic plates with planets and their satellites (including near 80 images of Uranus, Pluto and Neptune), about 1700 plates with minor planets and about 900 plates with comets. Plates were made during 1949-1999 using 11 telescopes of different focus, mostly the Double Wide-angle Astrograph (F/D=2000/400) and the Double Long-focus Astrograph (F/D=5500/400) of MAO NASU. Observational sites are Kyiv, Lviv (Ukraine), Biurakan (Armenia), Abastumani (Georgia), Mt. Maidanak (Uzbekistan), Quito (Equador). Tables contain data about the most significant numbers of plates sub-divided by years and objects. The database with metadata of plates (DBGPA) is available on the computer cluster of MAO (http://gua.db.ukr-vo.org) via open access. The database accumulates archives of four Ukrainian observatories, involving the UkrVO national project. Together with the archive managing system, the database serves as a test area for JDA - Joint Digital Archive - the core of the UkrVO.

Click nucleic acid ligation: applications in biology and nanotechnology.

PubMed

El-Sagheer, Afaf H; Brown, Tom

2012-08-21

Biochemical strategies that use a combination of synthetic oligonucleotides, thermostable DNA polymerases, and DNA ligases can produce large DNA constructs up to 1 megabase in length. Although these ambitious targets are feasible biochemically, comparable technologies for the chemical synthesis of long DNA strands lag far behind. The best available chemical approach is the solid-phase phosphoramidite method, which can be used to assemble DNA strands up to 150 bases in length. Beyond this point, deficiencies in the chemistry make it impossible to produce pure DNA. A possible alternative approach to the chemical synthesis of large DNA strands is to join together carefully purified synthetic oligonucleotides by chemical methods. Click ligation by the copper-catalyzed azide-alkyne (CuAAC) reaction could facilitate this process. In this Account, we describe the synthesis, characterization, and applications of oligonucleotides prepared by click ligation. The alkyne and azide oligonucleotide strands can be prepared by standard protocols, and the ligation reaction is compatible with a wide range of chemical modifications to DNA and RNA. We have employed click ligation to synthesize DNA constructs up to 300 bases in length and much longer sequences are feasible. When the resulting triazole linkage is placed in a PCR template, various DNA polymerases correctly copy the entire base sequence. We have also successfully demonstrated both in vitro transcription and rolling circle amplification through the modified linkage. This linkage has shown in vivo biocompatibility: an antibiotic resistance gene containing triazole linkages functions in E. coli . Using click ligation, we have synthesized hairpin ribozymes up to 100 nucleotides in length and a hammerhead ribozyme with the triazole linkage located at the substrate cleavage site. At the opposite end of the length scale, click-ligated, cyclic mini-DNA duplexes have been used as models to study base pairing. Cyclic duplexes have potential therapeutic applications. They have extremely high thermodynamic stability, have increased resistance to enzymatic degradation, and have been investigated as decoys for regulatory proteins. For potential nanotechnology applications, we have synthesized double stranded DNA catenanes by click ligation. Other researchers have studied covalently fixed multistranded DNA constructs including triplexes and quadruplexes.

Alternative Fuels Data Center: Video Download Help

Science.gov Websites

a Windows Media Video (WMV) link and select "Save Target As..." from the shortcut menu. To player on the screen. You can also expand the video to play in full-screen mode using Windows Media played in Windows Media Player. Download Windows Media Player. To watch videos on a Mac, double-click the

Facilitating Navigation Through Large Archives

NASA Technical Reports Server (NTRS)

Shelton, Robert O.; Smith, Stephanie L.; Troung, Dat; Hodgson, Terry R.

2005-01-01

Automated Visual Access (AVA) is a computer program that effectively makes a large collection of information visible in a manner that enables a user to quickly and efficiently locate information resources, with minimal need for conventional keyword searches and perusal of complex hierarchical directory systems. AVA includes three key components: (1) a taxonomy that comprises a collection of words and phrases, clustered according to meaning, that are used to classify information resources; (2) a statistical indexing and scoring engine; and (3) a component that generates a graphical user interface that uses the scoring data to generate a visual map of resources and topics. The top level of an AVA display is a pictorial representation of an information archive. The user enters the depicted archive by either clicking on a depiction of subject area cluster, selecting a topic from a list, or entering a query into a text box. The resulting display enables the user to view candidate information entities at various levels of detail. Resources are grouped spatially by topic with greatest generality at the top layer and increasing detail with depth. The user can zoom in or out of specific sites or into greater or lesser content detail.

Enabling Disabled Persons to Gain Access to Digital Media

NASA Technical Reports Server (NTRS)

Beach, Glenn; OGrady, Ryan

2011-01-01

A report describes the first phase in an effort to enhance the NaviGaze software to enable profoundly disabled persons to operate computers. (Running on a Windows-based computer equipped with a video camera aimed at the user s head, the original NaviGaze software processes the user's head movements and eye blinks into cursor movements and mouse clicks to enable hands-free control of the computer.) To accommodate large variations in movement capabilities among disabled individuals, one of the enhancements was the addition of a graphical user interface for selection of parameters that affect the way the software interacts with the computer and tracks the user s movements. Tracking algorithms were improved to reduce sensitivity to rotations and reduce the likelihood of tracking the wrong features. Visual feedback to the user was improved to provide an indication of the state of the computer system. It was found that users can quickly learn to use the enhanced software, performing single clicks, double clicks, and drags within minutes of first use. Available programs that could increase the usability of NaviGaze were identified. One of these enables entry of text by using NaviGaze as a mouse to select keys on a virtual keyboard.

[Eleven thesis on the archive of scientific research, for a new patrimonial and scientific policy].

PubMed

Müller, Bertrand

2015-12-01

Abstracting the main content of a recent report on the bad state of the archives of scientific research, this paper puts forward eleven thesis likely to feed, in this time of numeric transition to a new documentary regime and to a new patrimonial policy. The recent numeric conditions impose to set new archival pratices, more proactive, anticipative and prospective. Archives of scientific research must be thought in a double memorial and scientific dimension, and not only as a patrimonial or historical one.

Continuous, Large-Scale Processing of Seismic Archives for High-Resolution Monitoring of Seismic Activity and Seismogenic Properties

NASA Astrophysics Data System (ADS)

Waldhauser, F.; Schaff, D. P.

2012-12-01

Archives of digital seismic data recorded by seismometer networks around the world have grown tremendously over the last several decades helped by the deployment of seismic stations and their continued operation within the framework of monitoring earthquake activity and verification of the Nuclear Test-Ban Treaty. We show results from our continuing effort in developing efficient waveform cross-correlation and double-difference analysis methods for the large-scale processing of regional and global seismic archives to improve existing earthquake parameter estimates, detect seismic events with magnitudes below current detection thresholds, and improve real-time monitoring procedures. We demonstrate the performance of these algorithms as applied to the 28-year long seismic archive of the Northern California Seismic Network. The tools enable the computation of periodic updates of a high-resolution earthquake catalog of currently over 500,000 earthquakes using simultaneous double-difference inversions, achieving up to three orders of magnitude resolution improvement over existing hypocenter locations. This catalog, together with associated metadata, form the underlying relational database for a real-time double-difference scheme, DDRT, which rapidly computes high-precision correlation times and hypocenter locations of new events with respect to the background archive (http://ddrt.ldeo.columbia.edu). The DDRT system facilitates near-real-time seismicity analysis, including the ability to search at an unprecedented resolution for spatio-temporal changes in seismogenic properties. In areas with continuously recording stations, we show that a detector built around a scaled cross-correlation function can lower the detection threshold by one magnitude unit compared to the STA/LTA based detector employed at the network. This leads to increased event density, which in turn pushes the resolution capability of our location algorithms. On a global scale, we are currently building the computational framework for double-difference processing the combined parametric and waveform archives of the ISC, NEIC, and IRIS with over three million recorded earthquakes worldwide. Since our methods are scalable and run on inexpensive Beowulf clusters, periodic re-analysis of such archives may thus become a routine procedure to continuously improve resolution in existing global earthquake catalogs. Results from subduction zones and aftershock sequences of recent great earthquakes demonstrate the considerable social and economic impact that high-resolution images of active faults, when available in real-time, will have in the prompt evaluation and mitigation of seismic hazards. These results also highlight the need for consistent long-term seismic monitoring and archiving of records.

Bulk ZnO: Current Status, Challenges, and Prospects

DTIC Science & Technology

2009-04-01

von Wenckstern, H. Schmidt, M. Lorenz, and M. Grundmann, “Defects in virgin and N+-implanted ZnO single crystals studied by positron annihilation...characterization, and device applications of semiconductor and complex oxide thin films. He is a co-author of more than 50 papers in referred...REPLACE THIS LINE WITH YOUR PAPER IDENTIFICATION NUMBER (DOUBLE-CLICK HERE TO EDIT) < 1 Abstract— Rediscovered in the last decade, zinc oxide

ASF archive issues: Current status, past history, and questions for the future

NASA Technical Reports Server (NTRS)

Goula, Crystal A.; Wales, Carl

1994-01-01

The Alaska SAR Facility (ASF) collects, processes, archives, and distributes data from synthetic aperture radar (SAR) satellites in support of scientific research. ASF has been in operation since 1991 and presently has an archive of over 100 terabytes of data. ASF is performing an analysis of its magnetic tape storage system to ensure long-term preservation of this archive. Future satellite missions have the possibility of doubling to tripling the amounts of data that ASF acquires. ASF is examining the current data systems and the high volume storage, and exploring future concerns and solutions.

Cryogenic Tm: YAG Laser in the Near Infrared

DTIC Science & Technology

2015-05-29

Applications Group. The focus of his work at Lincoln Laboratory has been solid-state lasers including microchip lasers , external-cavity diode lasers ...REPLACE THIS LINE WITH YOUR PAPER IDENTIFICATION NUMBER (DOUBLE-CLICK HERE TO EDIT) < Cryogenic Tm:YAG Laser in the Near Infrared* Tso Yee Fan...Senior Member, IEEE, Juan R. Ochoa, and Patricia A. Reed Abstract- Thulium laser operation on the 3H4 - 3H6 transition at 823 nm has been demonstrated

Investigating the cellular fate of a DNA-targeted platinum-based anticancer agent by orthogonal double-click chemistry

PubMed Central

Qiao, Xin; Ding, Song; Liu, Fang; Kucera, Gregory L.

2014-01-01

Confocal fluorescence microscopy was used to study a platinum-based anticancer agent in intact NCI-H460 lung cancer cells. Orthogonal copper-catalyzed azide–alkyne cycloaddition (click) reactions were used to simultaneously determine the cell-cycle-specific localization of the azide-functionalized platinum–acridine agent 1 and monitor its effects on nucleic acid metabolism. Copper-catalyzed postlabeling showed advantages over copper-free click chemistry using a dibenzocyclooctyne (DIBO)-modified reporter dye, which produced high background levels in microscopic images and failed to efficiently label platinum adducts in chromatin. Compound 1 was successfully labeled with the fluorophore DIBO to yield 1* (characterized by in-line high-performance liquid chromatography/electrospray mass spectrometry). 1 and 1* show a high degree of colocalization in the confocal images, but the ability of 1* to target the (compacted) chromatin was markedly reduced, most likely owing to the steric bulk introduced by the DIBO tag. Nuclear platinum levels correlated inversely with the ability of the cells to synthesize DNA and cause cell cycle arrest, as confirmed by bivariate flow cytometry analysis. In addition, a decrease in the level of cellular transcription, shrinkage of the nucleolar regions, and redistribution of RNA into the cytosol were observed. Postlabeling in conjunction with colocalization experiments is a useful tool for studying the cell killing mechanism of this type of DNA-targeted agent. PMID:24407462

Archiving of Wideband Plasma Wave Data

NASA Technical Reports Server (NTRS)

Kurth, William S.

1997-01-01

Beginning with the third year of funding, we began a more ambitious archiving production effort, minimizing work on new software and concentrating on building representative archives of the missions mentioned above, recognizing that only a small percentage of the data from any one mission can be archived with reasonable effort. We concentrated on data from Dynamics Explorer and ISEE 1, archiving orbits or significant fractions of orbits which attempt to capture the essence of the mission and provide data which will hopefully be sufficient for ongoing and new research as well as to provide a reference to upcoming and current ISTP missions which will not fly in the same regions of space as the older missions and which will not have continuous wideband data. We archived approximately 181 Gigabytes of data, accounting for some 1582 hours of data. Included in these data are all of the AMPTE chemical releases, all of the Spacelab 2/PDP data obtained during the free-flight portion of its mission, as well as significant portions of the S3, DE-1, Imp-6, Hawkeye, Injun 5, and ISEE 1 and 2 data sets. Table 1 summarizes these data. All of the data archived are summarized in gif-formatted images of frequency-time spectrograms which are directly accessible via the internet. Each of the gif files are identified by year, day, and time as described in the Web page. This provides a user with a specific date/time in mind a way of determining very quickly if there is data for the interval in question and, by clicking on the file name, browsing the data. Alternately, a user can browse the data for interesting features and events simply by viewing each of the gif files. When a user finds data of interest, he/she can notify us by email of the time period involved. Based on the user's needs, we can provide data on a convenient medium or by ftp, or we can mount the appropriate data and provide access to our analysis tools via the network. We can even produce products such as plots or spectrograms in hardcopy form based on the specific request of the user.

Programmed database system at the Chang Gung Craniofacial Center: part II--digitizing photographs.

PubMed

Chuang, Shiow-Shuh; Hung, Kai-Fong; de Villa, Glenda H; Chen, Philip K T; Lo, Lun-Jou; Chang, Sophia C N; Yu, Chung-Chih; Chen, Yu-Ray

2003-07-01

The archival tools used for digital images in advertising are not to fulfill the clinic requisition and are just beginning to develop. The storage of a large amount of conventional photographic slides needs a lot of space and special conditions. In spite of special precautions, degradation of the slides still occurs. The most common degradation is the appearance of fungus flecks. With the recent advances in digital technology, it is now possible to store voluminous numbers of photographs on a computer hard drive and keep them for a long time. A self-programmed interface has been developed to integrate database and image browser system that can build and locate needed files archive in a matter of seconds with the click of a button. This system requires hardware and software were market provided. There are 25,200 patients recorded in the database that involve 24,331 procedures. In the image files, there are 6,384 patients with 88,366 digital pictures files. From 1999 through 2002, NT400,000 dollars have been saved using the new system. Photographs can be managed with the integrating Database and Browse software for database archiving. This allows labeling of the individual photographs with demographic information and browsing. Digitized images are not only more efficient and economical than the conventional slide images, but they also facilitate clinical studies.

USNO Image and Catalog Archive Server - Naval Oceanography Portal

Science.gov Websites

are here: Home › USNO › Astrometry › Optical/IR Products › USNO Image and Catalog Archive Server USNO Logo USNO Navigation Optical/IR Products NOMAD UCAC URAT USNO-B1.0 Double Stars Solar System Link Disclaimer This is an official U.S. Navy web site. Security & Privacy Policy Veterans Crisis

Impact of Mental Health Screening on Promoting Immediate Online Help-Seeking: Randomized Trial Comparing Normative Versus Humor-Driven Feedback.

PubMed

Choi, Isabella; Milne, David N; Deady, Mark; Calvo, Rafael A; Harvey, Samuel B; Glozier, Nick

2018-04-05

Given the widespread availability of mental health screening apps, providing personalized feedback may encourage people at high risk to seek help to manage their symptoms. While apps typically provide personal score feedback only, feedback types that are user-friendly and increase personal relevance may encourage further help-seeking. The aim of this study was to compare the effects of providing normative and humor-driven feedback on immediate online help-seeking, defined as clicking on a link to an external resource, and to explore demographic predictors that encourage help-seeking. An online sample of 549 adults were recruited using social media advertisements. Participants downloaded a smartphone app known as "Mindgauge" which allowed them to screen their mental wellbeing by completing standardized measures on Symptoms (Kessler 6-item Scale), Wellbeing (World Health Organization [Five] Wellbeing Index), and Resilience (Brief Resilience Scale). Participants were randomized to receive normative feedback that compared their scores to a reference group or humor-driven feedback that presented their scores in a relaxed manner. Those who scored in the moderate or poor ranges in any measure were encouraged to seek help by clicking on a link to an external online resource. A total of 318 participants scored poorly on one or more measures and were provided with an external link after being randomized to receive normative or humor-driven feedback. There was no significant difference of feedback type on clicking on the external link across all measures. A larger proportion of participants from the Wellbeing measure (170/274, 62.0%) clicked on the links than the Resilience (47/179, 26.3%) or Symptoms (26/75, 34.7%) measures (χ 2 =60.35, P<.001). There were no significant demographic factors associated with help-seeking for the Resilience or Wellbeing measures. Participants with a previous episode of poor mental health were less likely than those without such history to click on the external link in the Symptoms measure (P=.003, odds ratio [OR] 0.83, 95% CI 0.02-0.44), and younger adults were less likely to click on the link compared to older adults across all measures (P=.005, OR 0.44, 95% CI 0.25-0.78). This pilot study found that there was no difference between normative and humor-driven feedback on promoting immediate clicks to an external resource, suggesting no impact on online help-seeking. Limitations included: lack of personal score control group, limited measures of predictors and potential confounders, and the fact that other forms of professional help-seeking were not assessed. Further investigation into other predictors and factors that impact on help-seeking is needed. Australian New Zealand Clinical Trials Registry ACTRN12616000707460; https://www.anzctr.org.au/ Trial/Registration/TrialReview.aspx?id=370187 (Archived by WebCite at http://www.webcitation.org/6y8m8sVxr). ©Isabella Choi, David N Milne, Mark Deady, Rafael A Calvo, Samuel B Harvey, Nick Glozier. Originally published in JMIR Mental Health (http://mental.jmir.org), 05.04.2018.

Single-chain polybutadiene organometallic nanoparticles: an experimental and theoretical study† †Electronic supplementary information (ESI) available: Detailed experimental procedures and characterization of ONPs including: 1H and 13C NMR, SEC, DLS, DSC, TGA, UV-vis, GC-MS spectra and computational calculations. See DOI: 10.1039/c5sc04535e Click here for additional data file.

PubMed Central

Berkovich, Inbal; Mavila, Sudheendran; Iliashevsky, Olga; Kozuch, Sebastian

2016-01-01

High molecular weight polybutadienes and rhodium complexes were used to produce single chain organometallic nanoparticles. Irradiation of high cis-polybutadiene in the presence of a photosensitizer isomerised the double bonds to produce differing cis/trans ratios within the polymer. Notably, a higher cis percentage of carbon–carbon double bonds within the polymer structure led to faster binding of metal ions, as well as their faster removal by competing phosphine ligands. The experimental results were supported and rationalized by DFT computations. PMID:28936327

Disruption of the auditory response to a regular click train by a single, extra click.

PubMed

Lütkenhöner, Bernd; Patterson, Roy D

2015-06-01

It has been hypothesized that the steady-state response to a periodic sequence of clicks can be modeled as the superposition of responses to single clicks. Here, this hypothesis is challenged by presenting an extra click halfway between two consecutive clicks of a regular series, while measuring the auditory evoked field. After a solitary click at time zero, the click series sounded from 100 to 900 ms, with the extra click presented around 500 ms. The silent period between two stimulus sequences was 310-390 ms (uniformly distributed) so that one stimulation cycle lasted, on average, 1250 ms. Five different click rates between 20 and 60 Hz were examined. The disturbance caused by the extra click was revealed by subtracting the estimated steady-state response from the joint response to the click series and the extra click. The early peaks of the single-click response effectively coincide with same-polarity peaks of the 20-Hz steady-state response. Nevertheless, prediction of the latter from the former proved impossible. However, the 40-Hz steady-state response can be predicted reasonably well from the 20-Hz steady-state response. Somewhat surprisingly, the amplitude of the evoked response to the extra click grew when the click rate of the train was increased from 20 to 30 Hz; the opposite effect would have been expected from research on adaptation. The smaller amplitude at lower click rates might be explained by forward suppression. In this case, the apparent escape from suppression at higher rates might indicate that the clicks belonging to the periodic train are being integrated into an auditory stream, possibly in much the same manner as in classical stream segregation experiments.

Interoperability at ESA Heliophysics Science Archives: IVOA, HAPI and other implementations

NASA Astrophysics Data System (ADS)

Martinez-Garcia, B.; Cook, J. P.; Perez, H.; Fernandez, M.; De Teodoro, P.; Osuna, P.; Arnaud, M.; Arviset, C.

2017-12-01

The data of ESA heliophysics science missions are preserved at the ESAC Science Data Centre (ESDC). The ESDC aims for the long term preservation of those data, which includes missions such as Ulysses, Soho, Proba-2, Cluster, Double Star, and in the future, Solar Orbiter. Scientists have access to these data through web services, command line and graphical user interfaces for each of the corresponding science mission archives. The International Virtual Observatory Alliance (IVOA) provides technical standards that allow interoperability among different systems that implement them. By adopting some IVOA standards, the ESA heliophysics archives are able to share their data with those tools and services that are VO-compatible. Implementation of those standards can be found in the existing archives: Ulysses Final Archive (UFA) and Soho Science Archive (SSA). They already make use of VOTable format definition and Simple Application Messaging Protocol (SAMP). For re-engineered or new archives, the implementation of services through Table Access Protocol (TAP) or Universal Worker Service (UWS) will leverage this interoperability. This will be the case for the Proba-2 Science Archive (P2SA) and the Solar Orbiter Archive (SOAR). We present here which IVOA standards were already used by the ESA Heliophysics archives in the past and the work on-going.

Automated classification of dolphin echolocation click types from the Gulf of Mexico.

PubMed

Frasier, Kaitlin E; Roch, Marie A; Soldevilla, Melissa S; Wiggins, Sean M; Garrison, Lance P; Hildebrand, John A

2017-12-01

Delphinids produce large numbers of short duration, broadband echolocation clicks which may be useful for species classification in passive acoustic monitoring efforts. A challenge in echolocation click classification is to overcome the many sources of variability to recognize underlying patterns across many detections. An automated unsupervised network-based classification method was developed to simulate the approach a human analyst uses when categorizing click types: Clusters of similar clicks were identified by incorporating multiple click characteristics (spectral shape and inter-click interval distributions) to distinguish within-type from between-type variation, and identify distinct, persistent click types. Once click types were established, an algorithm for classifying novel detections using existing clusters was tested. The automated classification method was applied to a dataset of 52 million clicks detected across five monitoring sites over two years in the Gulf of Mexico (GOM). Seven distinct click types were identified, one of which is known to be associated with an acoustically identifiable delphinid (Risso's dolphin) and six of which are not yet identified. All types occurred at multiple monitoring locations, but the relative occurrence of types varied, particularly between continental shelf and slope locations. Automatically-identified click types from autonomous seafloor recorders without verifiable species identification were compared with clicks detected on sea-surface towed hydrophone arrays in the presence of visually identified delphinid species. These comparisons suggest potential species identities for the animals producing some echolocation click types. The network-based classification method presented here is effective for rapid, unsupervised delphinid click classification across large datasets in which the click types may not be known a priori.

Automated classification of dolphin echolocation click types from the Gulf of Mexico

PubMed Central

Roch, Marie A.; Soldevilla, Melissa S.; Wiggins, Sean M.; Garrison, Lance P.; Hildebrand, John A.

2017-01-01

Delphinids produce large numbers of short duration, broadband echolocation clicks which may be useful for species classification in passive acoustic monitoring efforts. A challenge in echolocation click classification is to overcome the many sources of variability to recognize underlying patterns across many detections. An automated unsupervised network-based classification method was developed to simulate the approach a human analyst uses when categorizing click types: Clusters of similar clicks were identified by incorporating multiple click characteristics (spectral shape and inter-click interval distributions) to distinguish within-type from between-type variation, and identify distinct, persistent click types. Once click types were established, an algorithm for classifying novel detections using existing clusters was tested. The automated classification method was applied to a dataset of 52 million clicks detected across five monitoring sites over two years in the Gulf of Mexico (GOM). Seven distinct click types were identified, one of which is known to be associated with an acoustically identifiable delphinid (Risso’s dolphin) and six of which are not yet identified. All types occurred at multiple monitoring locations, but the relative occurrence of types varied, particularly between continental shelf and slope locations. Automatically-identified click types from autonomous seafloor recorders without verifiable species identification were compared with clicks detected on sea-surface towed hydrophone arrays in the presence of visually identified delphinid species. These comparisons suggest potential species identities for the animals producing some echolocation click types. The network-based classification method presented here is effective for rapid, unsupervised delphinid click classification across large datasets in which the click types may not be known a priori. PMID:29216184

Click rates and silences of sperm whales at Kaikoura, New Zealand

NASA Astrophysics Data System (ADS)

Douglas, Lesley A.; Dawson, Stephen M.; Jaquet, Nathalie

2005-07-01

Analysis of the usual click rates of sperm whales (Physeter macrocephalus) at Kaikoura, New Zealand, confirms the potential for assessing abundance via ``click counting.'' Usual click rates over three dive cycles each of three photographically identified whales showed that 5 min averages of usual click rate did not differ significantly within dives, among dives of the same whale or among whales. Over the nine dives (n=13 728 clicks) mean usual click rate was 1.272 clicks s-1 (95% CI=0.151). On average, individual sperm whales at Kaikoura spent 60% of their time usual clicking in winter and in summer. There was no evidence that whale identity or stage of the dive recorded affects significantly the percentage of time spent usual clicking. Differences in vocal behavior among sperm whale populations worldwide indicate that estimates of abundance that are based on click rates need to based on data from the population of interest, rather than from another population or some global average.

Switchable silver mirrors with long memory effects† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c4sc01912a Click here for additional data file. Click here for additional data file.

PubMed Central

Park, Chihyun; Seo, Seogjae; Shin, Haijin; Sarwade, Bhimrao D.; Na, Jongbeom

2015-01-01

An electrochemically stable and bistable switchable mirror was achieved for the first time by introducing (1) a thiol-modified indium tin oxide (ITO) electrode for the stabilization of the metallic film and (2) ionic liquids as an anion-blocking layer, to achieve a long memory effect. The growth of the metallic film was denser and faster at the thiol-modified ITO electrode than at a bare ITO electrode. The electrochemical stability of the metallic film on the thiol-modified ITO was enhanced, maintaining the metallic state without rupture. In the voltage-off state, the metal film maintained bistability for a long period (>2 h) when ionic liquids were introduced as electrolytes for the switchable mirror. The electrical double layer in the highly viscous ionic liquid electrolyte seemed to effectively form a barrier to the bromide ions, to protect the metal thin film from them when in the voltage-off state. PMID:28936310

Development of an image operation system with a motion sensor in dental radiology.

PubMed

Sato, Mitsuru; Ogura, Toshihiro; Yasumoto, Yoshiaki; Kadowaki, Yuta; Hayashi, Norio; Doi, Kunio

2015-07-01

During examinations and/or treatment, a dentist in the examination room needs to view images with a proper display system. However, they cannot operate the image display system by hands, because dentists always wear gloves to be kept their hands away from unsanitized materials. Therefore, we developed a new image operating system that uses a motion sensor. We used the Leap motion sensor technique to read the hand movements of a dentist. We programmed the system using C++ to enable various movements of the display system, i.e., click, double click, drag, and drop. Thus, dentists with their gloves on in the examination room can control dental and panoramic images on the image display system intuitively and quickly with movement of their hands only. We investigated the time required with the conventional method using a mouse and with the new method using the finger operation. The average operation time with the finger method was significantly shorter than that with the mouse method. This motion sensor method, with appropriate training for finger movements, can provide a better operating performance than the conventional mouse method.

Hearing Sensation Levels of Emitted Biosonar Clicks in an Echolocating Atlantic Bottlenose Dolphin

PubMed Central

Li, Songhai; Nachtigall, Paul E.; Breese, Marlee; Supin, Alexander Ya.

2012-01-01

Emitted biosonar clicks and auditory evoked potential (AEP) responses triggered by the clicks were synchronously recorded during echolocation in an Atlantic bottlenose dolphin (Tursiops truncatus) trained to wear suction-cup EEG electrodes and to detect targets by echolocation. Three targets with target strengths of −34, −28, and −22 dB were used at distances of 2 to 6.5 m for each target. The AEP responses were sorted according to the corresponding emitted click source levels in 5-dB bins and averaged within each bin to extract biosonar click-related AEPs from noise. The AEP amplitudes were measured peak-to-peak and plotted as a function of click source levels for each target type, distance, and target-present or target-absent condition. Hearing sensation levels of the biosonar clicks were evaluated by comparing the functions of the biosonar click-related AEP amplitude-versus-click source level to a function of external (in free field) click-related AEP amplitude-versus-click sound pressure level. The results indicated that the dolphin's hearing sensation levels to her own biosonar clicks were equal to that of external clicks with sound pressure levels 16 to 36 dB lower than the biosonar click source levels, varying with target type, distance, and condition. These data may be assumed to indicate that the bottlenose dolphin possesses effective protection mechanisms to isolate the self-produced intense biosonar beam from the animal's ears during echolocation. PMID:22238654

Hearing sensation levels of emitted biosonar clicks in an echolocating Atlantic bottlenose dolphin.

PubMed

Li, Songhai; Nachtigall, Paul E; Breese, Marlee; Supin, Alexander Ya

2012-01-01

Emitted biosonar clicks and auditory evoked potential (AEP) responses triggered by the clicks were synchronously recorded during echolocation in an Atlantic bottlenose dolphin (Tursiops truncatus) trained to wear suction-cup EEG electrodes and to detect targets by echolocation. Three targets with target strengths of -34, -28, and -22 dB were used at distances of 2 to 6.5 m for each target. The AEP responses were sorted according to the corresponding emitted click source levels in 5-dB bins and averaged within each bin to extract biosonar click-related AEPs from noise. The AEP amplitudes were measured peak-to-peak and plotted as a function of click source levels for each target type, distance, and target-present or target-absent condition. Hearing sensation levels of the biosonar clicks were evaluated by comparing the functions of the biosonar click-related AEP amplitude-versus-click source level to a function of external (in free field) click-related AEP amplitude-versus-click sound pressure level. The results indicated that the dolphin's hearing sensation levels to her own biosonar clicks were equal to that of external clicks with sound pressure levels 16 to 36 dB lower than the biosonar click source levels, varying with target type, distance, and condition. These data may be assumed to indicate that the bottlenose dolphin possesses effective protection mechanisms to isolate the self-produced intense biosonar beam from the animal's ears during echolocation.

Intra- versus intermolecular electron transfer in radical nucleophilic aromatic substitution of dihalo(hetero)arenes – a tool for estimating π-conjugation in aromatic systems† †Electronic supplementary information (ESI) available: Experimental details and procedures, 1H and 13C NMR data, GC traces and mass spectra. CCDC 1526301 and 1526302. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c7sc00100b Click here for additional data file. Click here for additional data file.

PubMed Central

Janhsen, B.; Daniliuc, C. G.

2017-01-01

In this paper, the application of the double radical nucleophilic aromatic substitution (SRN1) in various dihalogenated, mostly diiodinated, π-conjugated systems as a tool for qualitatively estimating their π-conjugation is described. This approach uses electron delocalisation as a measure of π-conjugation. Electron injection into the π-system is achieved via reaction of an intermediate aryl radical, itself generated from a dihalogenated π-system via SET-reduction of the C–I bond and subsequent reaction with a thiolate anion. The generated arene radical anion can then further react with the second aryl-halogen moiety within the π-system via an intramolecular electron transfer process. The efficiency of this intramolecular electron transfer is related to the π-conjugation of the radical anion. If the π-conjugation within the aromatic unit is weak, the arene radical anion reacts via an intermolecular ET with the starting dihalide. The intramolecular ET process delivers a product of a double SRN1 substitution whereas the intermolecular ET pathway provides a product of a mono- SRN1 substitution. By simple product analysis of mono- versus double substitution, π-conjugation can be qualitatively evaluated. This mechanistic tool is applied to various dihalogenated π-conjugated systems and the results are discussed within the context of π-conjugation. The conjugation mode within the π-system and the length of the aromatic system are varied, and the effect of relative positioning of the two halides within small π-systems is also addressed. PMID:28580099

11. Photocopy of 1872 photograph by Eadweard Muybridge in Stanford ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

11. Photocopy of 1872 photograph by Eadweard Muybridge in Stanford University Archives, PC 6. NORTHWEST DOUBLE PARLOR, LOOKING NORTH - Leland Stanford House, 800 N Street, Sacramento, Sacramento County, CA

Neural representation of the self-heard biosonar click in bottlenose dolphins (Tursiops truncatus).

PubMed

Finneran, James J; Mulsow, Jason; Houser, Dorian S; Schlundt, Carolyn E

2017-05-01

The neural representation of the dolphin broadband biosonar click was investigated by measuring auditory brainstem responses (ABRs) to "self-heard" clicks masked with noise bursts having various high-pass cutoff frequencies. Narrowband ABRs were obtained by sequentially subtracting responses obtained with noise having lower high-pass cutoff frequencies from those obtained with noise having higher cutoff frequencies. For comparison to the biosonar data, ABRs were also measured in a passive listening experiment, where external clicks and masking noise were presented to the dolphins and narrowband ABRs were again derived using the subtractive high-pass noise technique. The results showed little change in the peak latencies of the ABR to the self-heard click from 28 to 113 kHz; i.e., the high-frequency neural responses to the self-heard click were delayed relative to those of an external, spectrally "pink" click. The neural representation of the self-heard click is thus highly synchronous across the echolocation frequencies and does not strongly resemble that of a frequency modulated downsweep (i.e., decreasing-frequency chirp). Longer ABR latencies at higher frequencies are hypothesized to arise from spectral differences between self-heard clicks and external clicks, forward masking from previously emitted biosonar clicks, or neural inhibition accompanying the emission of clicks.

Neural representation of the self-heard biosonar click in bottlenose dolphins (Tursiops truncatus)

PubMed Central

Finneran, James J.; Mulsow, Jason; Houser, Dorian S.; Schlundt, Carolyn E.

2017-01-01

The neural representation of the dolphin broadband biosonar click was investigated by measuring auditory brainstem responses (ABRs) to “self-heard” clicks masked with noise bursts having various high-pass cutoff frequencies. Narrowband ABRs were obtained by sequentially subtracting responses obtained with noise having lower high-pass cutoff frequencies from those obtained with noise having higher cutoff frequencies. For comparison to the biosonar data, ABRs were also measured in a passive listening experiment, where external clicks and masking noise were presented to the dolphins and narrowband ABRs were again derived using the subtractive high-pass noise technique. The results showed little change in the peak latencies of the ABR to the self-heard click from 28 to 113 kHz; i.e., the high-frequency neural responses to the self-heard click were delayed relative to those of an external, spectrally “pink” click. The neural representation of the self-heard click is thus highly synchronous across the echolocation frequencies and does not strongly resemble that of a frequency modulated downsweep (i.e., decreasing-frequency chirp). Longer ABR latencies at higher frequencies are hypothesized to arise from spectral differences between self-heard clicks and external clicks, forward masking from previously emitted biosonar clicks, or neural inhibition accompanying the emission of clicks. PMID:28599518

The ZeroAccess Auto-Clicking and Search-Hijacking Click Fraud Modules

DTIC Science & Technology

2013-12-16

payloads and instead began distributing Bitcoin miners and click fraud modules.3 From a technical perspective, the primary click fraud malware used in...this era operated in the indiscriminate “auto-clicking” fashion we describe in Section 5. Alongside the click fraud and Bitcoin payloads, ZeroAccess

Echolocation-Based Foraging by Harbor Porpoises and Sperm Whales, Including Effects on Noise and Acoustic Propagation

DTIC Science & Technology

2008-09-01

Behavioural Point Process Data 234 Appendix B: Matlab Code 258 Matlab Code Used in Chapter 2 (Porpoise Prey Capture Analysis) 258 Click Extraction and...Measurement of Click Properties 258 Envelope-based Click Detector 262 Matlab Code Used in Chapter 3 (Transmission Loss in Porpoise Habitats) ..267...Click Extraction from Data Wavefiles 267 Click Level Determination (Grand Manan Datasets) 270 Click Level Determination (Danish Datasets) 287 Matlab

National Centers for Environmental Prediction

Science.gov Websites

NAM Specifications/References [<--click here] Rapid Refresh (RAP) [<--click here] High -Resolution Rapid Refresh (HRRR) [<--click here] Short-range Ensemble Forecast (SREF) system [<--click

Stereotypical rapid source level regulation in the harbour porpoise biosonar

NASA Astrophysics Data System (ADS)

Linnenschmidt, Meike; Kloepper, Laura N.; Wahlberg, Magnus; Nachtigall, Paul E.

2012-09-01

Some odontocetes and bats vary both click intensity and receiver sensitivity during echolocation, depending on target range. It is not known how this so-called automatic gain control is regulated by the animal. The source level of consecutive echolocation clicks from a harbour porpoise was measured with a hydrophone array while the animal detected an aluminium cylinder at 2, 4 or 8 m distance in a go/no-go paradigm. On-axis clicks had source levels of 145-174 dB re 1 μPa peak-to-peak. During target-present trials the click trains reached comparable source levels independent of the range to the target after three clicks. After an additional click, the source level was reduced for the 2 and 4 m trials until it equalled the one-way transmission loss. During target-absent trials, the source level remained high throughout the entire click train. Given typical values of harbour porpoise inter-click intervals, the source level reduction commenced within a few 100 ms from the first click in the click train. This may indicate a sub-cortically regulated source level regulation in the harbour porpoise.

10. Photocopy of 1872 photograph by Eadweard Muybridge in Stanford ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. Photocopy of 1872 photograph by Eadweard Muybridge in Stanford University Archives, PC 6. NORTHWEST DOUBLE PARLOR, LOOKING SOUTH (Present Chapel Space) - Leland Stanford House, 800 N Street, Sacramento, Sacramento County, CA

Dual mechanisms in the perceptual processing of click train temporal regularity.

PubMed

Phillips, Dennis P; Dingle, Rachel N; Hall, Susan E; Jang, Moragh

2012-07-01

Two experiments measured human sensitivity to temporal jitter in 25-click trains with inter-click intervals (ICIs) between 5 and 100 ms. In a naturalistic experiment using wideband clicks, jitter thresholds were a nonmonotonic function of ICI, peaking for ICIs near 40-60 ms. In a subsequent experiment, clicks were high-passed and presented against a low-frequency noise masker. Jitter threshold vs ICI functions lost the positive slope over short ICIs but retained the negative slope at long ICIs. The same behavior was seen in click rate discrimination tasks. Different processes mediate regularity analysis for click trains with ICIs above and below 40-60 ms.

WPC Maximum Heat Index Forecasts

Science.gov Websites

Forecasts for Western US CLICK ON MAPS FOR MAXIMUM HEAT INDEX AND PROBABILITY FORECASTS FROM SUN MAY 27 2018 02 CLICK to view SAT JUN 02 forecast SUN JUN 03 CLICK to view SUN JUN 03 forecast SUN JUN 03 CLICK to view SUN JUN 03 forecast SUN JUN 03 CLICK to view SUN JUN 03 forecast SUN JUN 03 CLICK to view SUN JUN

Assessing the biocompatibility of click-linked DNA in Escherichia coli

PubMed Central

Sanzone, A. Pia; El-Sagheer, Afaf H.; Brown, Tom; Tavassoli, Ali

2012-01-01

The biocompatibility of a triazole mimic of the DNA phosphodiester linkage in Escherichia coli has been evaluated. The requirement for selective pressure on the click-containing gene was probed via a plasmid containing click DNA backbone linkages in each strand of the gene encoding the fluorescent protein mCherry. The effect of proximity of the click linkers on their biocompatibility was also probed by placing two click DNA linkers 4-bp apart at the region encoding the fluorophore of the fluorescent protein. The resulting click-containing plasmid was found to encode mCherry in E. coli at a similar level to the canonical equivalent. The ability of the cellular machinery to read through click-linked DNA was further probed by using the above click-linked plasmid to express mCherry using an in vitro transcription/translation system, and found to also be similar to that from canonical DNA. The yield and fluorescence of recombinant mCherry expressed from the click-linked plasmid was also compared to that from the canonical equivalent, and found to be the same. The biocompatibility of click DNA ligation sites at close proximity in a non-essential gene demonstrated in E. coli suggests the possibility of using click DNA ligation for the enzyme-free assembly of chemically modified genes and genomes. PMID:22904087

What clicks actually mean: Exploring digital news user practices.

PubMed

Kormelink, Tim Groot; Meijer, Irene Costera

2018-05-01

This article problematizes the relationship between clicks and audience interests. Clicking patterns are often seen as evidence that news users are mostly interested in junk news, leading to concerns about the state of journalism and the implications for society. Asking and observing how 56 users actually browse news and what clicking and not clicking mean to them, we identified 30 distinct considerations for (not) clicking and classified them into three categories: cognitive, affective and pragmatic. The results suggest, first, that interest is too crude a term to account for the variety of people's considerations for (not) clicking. Second, even if one aims for roughly estimating people's news interests, clicks are a flawed instrument because a lack of clicking does not measure people's lack of interest in news. Third, taking users' browsing patterns seriously could help bridge the gap between what people need as citizens and what they actually consume. Finally, we argue that all metrics should be critically assessed from a user perspective rather than taken at face value.

What clicks actually mean: Exploring digital news user practices

PubMed Central

Kormelink, Tim Groot; Meijer, Irene Costera

2017-01-01

This article problematizes the relationship between clicks and audience interests. Clicking patterns are often seen as evidence that news users are mostly interested in junk news, leading to concerns about the state of journalism and the implications for society. Asking and observing how 56 users actually browse news and what clicking and not clicking mean to them, we identified 30 distinct considerations for (not) clicking and classified them into three categories: cognitive, affective and pragmatic. The results suggest, first, that interest is too crude a term to account for the variety of people’s considerations for (not) clicking. Second, even if one aims for roughly estimating people’s news interests, clicks are a flawed instrument because a lack of clicking does not measure people’s lack of interest in news. Third, taking users’ browsing patterns seriously could help bridge the gap between what people need as citizens and what they actually consume. Finally, we argue that all metrics should be critically assessed from a user perspective rather than taken at face value. PMID:29782573

Stereotypical rapid source level regulation in the harbour porpoise biosonar.

PubMed

Linnenschmidt, Meike; Kloepper, Laura N; Wahlberg, Magnus; Nachtigall, Paul E

2012-09-01

Some odontocetes and bats vary both click intensity and receiver sensitivity during echolocation, depending on target range. It is not known how this so-called automatic gain control is regulated by the animal. The source level of consecutive echolocation clicks from a harbour porpoise was measured with a hydrophone array while the animal detected an aluminium cylinder at 2, 4 or 8 m distance in a go/no-go paradigm. On-axis clicks had source levels of 145-174 dB re 1 μPa peak-to-peak. During target-present trials the click trains reached comparable source levels independent of the range to the target after three clicks. After an additional click, the source level was reduced for the 2 and 4 m trials until it equalled the one-way transmission loss. During target-absent trials, the source level remained high throughout the entire click train. Given typical values of harbour porpoise inter-click intervals, the source level reduction commenced within a few 100 ms from the first click in the click train. This may indicate a sub-cortically regulated source level regulation in the harbour porpoise.

AutoClickChem: click chemistry in silico.

PubMed

Durrant, Jacob D; McCammon, J Andrew

2012-01-01

Academic researchers and many in industry often lack the financial resources available to scientists working in "big pharma." High costs include those associated with high-throughput screening and chemical synthesis. In order to address these challenges, many researchers have in part turned to alternate methodologies. Virtual screening, for example, often substitutes for high-throughput screening, and click chemistry ensures that chemical synthesis is fast, cheap, and comparatively easy. Though both in silico screening and click chemistry seek to make drug discovery more feasible, it is not yet routine to couple these two methodologies. We here present a novel computer algorithm, called AutoClickChem, capable of performing many click-chemistry reactions in silico. AutoClickChem can be used to produce large combinatorial libraries of compound models for use in virtual screens. As the compounds of these libraries are constructed according to the reactions of click chemistry, they can be easily synthesized for subsequent testing in biochemical assays. Additionally, in silico modeling of click-chemistry products may prove useful in rational drug design and drug optimization. AutoClickChem is based on the pymolecule toolbox, a framework that may facilitate the development of future python-based programs that require the manipulation of molecular models. Both the pymolecule toolbox and AutoClickChem are released under the GNU General Public License version 3 and are available for download from http://autoclickchem.ucsd.edu.

AutoClickChem: Click Chemistry in Silico

PubMed Central

Durrant, Jacob D.; McCammon, J. Andrew

2012-01-01

Academic researchers and many in industry often lack the financial resources available to scientists working in “big pharma.” High costs include those associated with high-throughput screening and chemical synthesis. In order to address these challenges, many researchers have in part turned to alternate methodologies. Virtual screening, for example, often substitutes for high-throughput screening, and click chemistry ensures that chemical synthesis is fast, cheap, and comparatively easy. Though both in silico screening and click chemistry seek to make drug discovery more feasible, it is not yet routine to couple these two methodologies. We here present a novel computer algorithm, called AutoClickChem, capable of performing many click-chemistry reactions in silico. AutoClickChem can be used to produce large combinatorial libraries of compound models for use in virtual screens. As the compounds of these libraries are constructed according to the reactions of click chemistry, they can be easily synthesized for subsequent testing in biochemical assays. Additionally, in silico modeling of click-chemistry products may prove useful in rational drug design and drug optimization. AutoClickChem is based on the pymolecule toolbox, a framework that may facilitate the development of future python-based programs that require the manipulation of molecular models. Both the pymolecule toolbox and AutoClickChem are released under the GNU General Public License version 3 and are available for download from http://autoclickchem.ucsd.edu. PMID:22438795

The new Planetary Science Archive (PSA): Exploration and discovery of scientific datasets from ESA's planetary missions

NASA Astrophysics Data System (ADS)

Martinez, Santa; Besse, Sebastien; Heather, Dave; Barbarisi, Isa; Arviset, Christophe; De Marchi, Guido; Barthelemy, Maud; Docasal, Ruben; Fraga, Diego; Grotheer, Emmanuel; Lim, Tanya; Macfarlane, Alan; Rios, Carlos; Vallejo, Fran; Saiz, Jaime; ESDC (European Space Data Centre) Team

2016-10-01

The Planetary Science Archive (PSA) is the European Space Agency's (ESA) repository of science data from all planetary science and exploration missions. The PSA provides access to scientific datasets through various interfaces at http://archives.esac.esa.int/psa. All datasets are scientifically peer-reviewed by independent scientists, and are compliant with the Planetary Data System (PDS) standards. The PSA is currently implementing a number of significant improvements, mostly driven by the evolution of the PDS standard, and the growing need for better interfaces and advanced applications to support science exploitation. The newly designed PSA will enhance the user experience and will significantly reduce the complexity for users to find their data promoting one-click access to the scientific datasets with more specialised views when needed. This includes a better integration with Planetary GIS analysis tools and Planetary interoperability services (search and retrieve data, supporting e.g. PDAP, EPN-TAP). It will be also up-to-date with versions 3 and 4 of the PDS standards, as PDS4 will be used for ESA's ExoMars and upcoming BepiColombo missions. Users will have direct access to documentation, information and tools that are relevant to the scientific use of the dataset, including ancillary datasets, Software Interface Specification (SIS) documents, and any tools/help that the PSA team can provide. A login mechanism will provide additional functionalities to the users to aid / ease their searches (e.g. saving queries, managing default views). This contribution will introduce the new PSA, its key features and access interfaces.

Click chemistry in the Development of Contrast Agents for Magnetic Resonance Imaging

PubMed Central

Hapuarachchige, Sudath; Artemov, Dmitri

2016-01-01

Click chemistry provides fast, convenient, versatile and reliable chemical reactions that take place between pairs of functional groups of small molecules that can be purified without chromatographic methods. Due to the fast kinetics and low or no elimination of byproducts, click chemistry is a promising approach that is rapidly gaining acceptance in drug discovery, radiochemistry, bioconjugation, and nanoscience applications. Increasing use of click chemistry in synthetic procedures or as a bioconjugation technique in diagnostic imaging is occurring because click reactions are fast, provide a quantitative yield, and produce minimal amount of nontoxic byproducts. This review summarizes the recent application of click chemistry in magnetic resonance imaging and discusses the directions for applying novel click reactions and strategies for further improving MRI performance. PMID:27748712

Visual PEF Reader - VIPER

NASA Technical Reports Server (NTRS)

Luo, Victor; Khanampornpan, Teerapat; Boehmer, Rudy A.; Kim, Rachel Y.

2011-01-01

This software graphically displays all pertinent information from a Predicted Events File (PEF) using the Java Swing framework, which allows for multi-platform support. The PEF is hard to weed through when looking for specific information and it is a desire for the MRO (Mars Reconn aissance Orbiter) Mission Planning & Sequencing Team (MPST) to have a different way to visualize the data. This tool will provide the team with a visual way of reviewing and error-checking the sequence product. The front end of the tool contains much of the aesthetically appealing material for viewing. The time stamp is displayed in the top left corner, and highlighted details are displayed in the bottom left corner. The time bar stretches along the top of the window, and the rest of the space is allotted for blocks and step functions. A preferences window is used to control the layout of the sections along with the ability to choose color and size of the blocks. Double-clicking on a block will show information contained within the block. Zooming into a certain level will graphically display that information as an overlay on the block itself. Other functions include using hotkeys to navigate, an option to jump to a specific time, enabling a vertical line, and double-clicking to zoom in/out. The back end involves a configuration file that allows a more experienced user to pre-define the structure of a block, a single event, or a step function. The individual will have to determine what information is important within each block and what actually defines the beginning and end of a block. This gives the user much more flexibility in terms of what the tool is searching for. In addition to the configurability, all the settings in the preferences window are saved in the configuration file as well

Effect of Broadband Nature of Marine Mammal Echolocation Clicks on Click-Based Population Density Estimates

DTIC Science & Technology

2014-09-30

initially on beaked whales (Blainville’s or Cuvier’s), for which high quality click recordings of clicks are available from DTAG data, as these are known...will be applied also to other species such as sperm whale (Physeter macrocephalus) (whose high source level assures long range detection and amplifies...REFERENCES Gillespie, D. and Leaper, R. (1996). Detection of sperm whale Physeter macrocephalus clicks and discrimination of individual vocalizations

Effect of Broadband Nature of Marine Mammal Echolocation Clicks on Click-Based Population Density Estimates

DTIC Science & Technology

2014-09-30

research will focus initially on beaked whales (Blainville’s or Cuvier’s), for which high quality click recordings of clicks are available from DTAG...The same methodology will be applied also to other species such as sperm whale (Physeter macrocephalus) (whose high source level assures long range...Thomas, University of St. Andrews). REFERENCES Gillespie, D. and Leaper, R. (1996). Detection of sperm whale Physeter macrocephalus clicks and

The function of male sperm whale slow clicks in a high latitude habitat: communication, echolocation, or prey debilitation?

PubMed

Oliveira, Cláudia; Wahlberg, Magnus; Johnson, Mark; Miller, Patrick J O; Madsen, Peter T

2013-05-01

Sperm whales produce different click types for echolocation and communication. Usual clicks and buzzes appear to be used primarily in foraging while codas are thought to function in social communication. The function of slow clicks is less clear, but they appear to be produced by males at higher latitudes, where they primarily forage solitarily, and on the breeding grounds, where they roam between groups of females. Here the behavioral context in which these vocalizations are produced and the function they may serve was investigated. Ninety-nine hours of acoustic and diving data were analyzed from sound recording tags on six male sperm whales in Northern Norway. The 755 slow clicks detected were produced by tagged animals at the surface (52%), ascending from a dive (37%), and during the bottom phase (11%), but never during the descent. Slow clicks were not associated with the production of buzzes, other echolocation clicks, or fast maneuvering that would indicate foraging. Some slow clicks were emitted in seemingly repetitive temporal patterns supporting the hypothesis that the function for slow clicks on the feeding grounds is long range communication between males, possibly relaying information about individual identity or behavioral states.

Click chemistry for the conservation of cellular structures and fluorescent proteins: ClickOx.

PubMed

Löschberger, Anna; Niehörster, Thomas; Sauer, Markus

2014-05-01

Reactive oxygen species (ROS), including hydrogen peroxide, are known to cause structural damage not only in living, but also in fixed, cells. Copper-catalyzed azide-alkyne cycloaddition (click chemistry) is known to produce ROS. Therefore, fluorescence imaging of cellular structures, such as the actin cytoskeleton, remains challenging when combined with click chemistry protocols. In addition, the production of ROS substantially weakens the fluorescence signal of fluorescent proteins. This led us to develop ClickOx, which is a new click chemistry protocol for improved conservation of the actin structure and better conservation of the fluorescence signal of green fluorescent protein (GFP)-fusion proteins. Herein we demonstrate that efficient oxygen removal by addition of an enzymatic oxygen scavenger system (ClickOx) considerably reduces ROS-associated damage during labeling of nascent DNA with ATTO 488 azide by Cu(I)-catalyzed click chemistry. Standard confocal and super-resolution fluorescence images of phalloidin-labeled actin filaments and GFP/yellow fluorescent protein-labeled cells verify the conservation of the cytoskeleton microstructure and fluorescence intensity, respectively. Thus, ClickOx can be used advantageously for structure preservation in conventional and most notably in super-resolution microscopy methods. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A versatile platform for precise synthesis of asymmetric molecular brush in one shot.

PubMed

Xu, Binbin; Feng, Chun; Huang, Xiaoyu

2017-08-24

Asymmetric molecular brushes emerge as a unique class of nanostructured polymers, while their versatile synthesis keeps a challenge for chemists. Here we show the synthesis of well-defined asymmetric molecular double-brushes comprising two different side chains linked to the same repeat unit along the backbone by one-pot concurrent atom transfer radical polymerization (ATRP) and Cu-catalyzed azide/alkyne cycloaddition (CuAAC) reaction. The double-brushes are based on a poly(Br-acrylate-alkyne) homopolymer possessing an alkynyl for CuAAC reaction and a 2-bromopropionate initiating group for ATRP in each repeat unit. The versatility of this one-shot approach is demonstrated by CuAAC reaction of alkynyl/poly(ethylene oxide)-N 3 and ATRP of various monomers. We also show the quantitative conversion of pentafluorophenyl ester groups to amide groups in side chains, allowing for the further fabrication of diverse building blocks. This work provides a versatile platform for facile synthesis of Janus-type double-brushes with structural and functional control, in a minimum number of reactions.Producing well-defined polymer compositions and structures facilitates their use in many different applications. Here the authors show the synthesis of well-defined asymmetric double-brushes by a one-pot concurrent atom transfer radical polymerization and Cu-catalyzed Click reaction.

Evaluation of tooth-click triggering and speech recognition in assistive technology for computer access.

PubMed

Simpson, Tyler; Gauthier, Michel; Prochazka, Arthur

2010-02-01

Computer access can play an important role in employment and leisure activities following spinal cord injury. The authors' prior work has shown that a tooth-click detecting device, when paired with an optical head mouse, may be used by people with tetraplegia for controlling cursor movement and mouse button clicks. To compare the efficacy of tooth clicks to speech recognition and that of an optical head mouse to a gyrometer head mouse for cursor and mouse button control of a computer. Six able-bodied and 3 tetraplegic subjects used the devices listed above to produce cursor movements and mouse clicks in response to a series of prompts displayed on a computer. The time taken to move to and click on each target was recorded. The use of tooth clicks in combination with either an optical head mouse or a gyrometer head mouse can provide hands-free cursor movement and mouse button control at a speed of up to 22% of that of a standard mouse. Tooth clicks were significantly faster at generating mouse button clicks than speech recognition when paired with either type of head mouse device. Tooth-click detection performed better than speech recognition when paired with both the optical head mouse and the gyrometer head mouse. Such a system may improve computer access for people with tetraplegia.

Archive of post-Hurricane Isabel coastal oblique aerial photographs collected during U.S. Geological Survey Field Activity 03CCH01 from Ocean City, Maryland, to Fort Caswell, North Carolina and Inland from Waynesboro to Redwood, Virginia, September 21 - 23, 2003

USGS Publications Warehouse

Subino, Janice A.; Morgan, Karen L.M.; Krohn, M. Dennis; Dadisman, Shawn V.

2013-01-01

On September 21 - 23, 2003, the United States Geological Survey (USGS) conducted an oblique aerial photographic survey along the Atlantic coast from Ocean City, Md., to Fort Caswell, N.C., and inland oblique aerial photographic survey from Waynesboro to Redwood, Va., aboard a Navajo Piper twin-engine airplane. The coastal survey was conducted at an altitude of 500 feet (ft) and approximately 1,000 ft offshore. For the inland photos, the aircraft tried to stay approximately 500 ft above the terrain. These coastal photos were used to document coastal changes like beach erosion and overwash caused by Hurricane Isabel, while the inland photos looked for potential landslides caused by heavy rains. The photos may also be used as baseline data for future coastal change analysis. The USGS and the National Aeronautics and Space Administration (NASA) surveyed the impact zone of Hurricane Isabel to better understand the changes in vulnerability of the Nation’s coasts to extreme storms (Morgan, 2009). This report serves as an archive of photographs collected during the September 21 - 23, 2003, post-Hurricane Isabel coastal and inland oblique aerial survey along with associated survey maps, KML files, navigation files, digital Field Activity Collection System (FACS) logs, and Federal Geographic Data Committee (FGDC) metadata. Refer to the Acronyms page for expansions of all acronyms and abbreviations used in this report. The USGS St. Petersburg Coastal and Marine Science Center (SPCMSC) assigns a unique identifier to each cruise or field activity. For example, 03CCH01 tells us the data were collected in 2003 for the Coastal Change Hazards (CCH) study and the data were collected during the first field activity for that project in that calendar year. Refer to http://walrus.wr.usgs.gov/infobank/programs/html/definition/activity.html for a detailed description of the method used to assign the ID number. The photographs provided here are Joint Photographic Experts Group (JPEG) scanned images of the analog 35 millimeter (mm) color positive slides. The photograph locations are estimates of the location of the plane (see the Navigation page). The metadata values for photo creation time, GPS latitude, GPS longitude, GPS position (latitude and longitude), keywords, credit, artist, caption, copyright, and contact were added to each photograph's EXIF header using EXIFtool (Subino and others, 2012). Photographs can be opened directly with any JPEG-compatible image viewer by clicking on a thumbnail on the contact sheet, or, when viewing the Google Earth KML file, by clicking on the marker and then clicking on either the thumbnail or the link below the thumbnail. Nathaniel Plant (USGS - St. Petersburg, Fla.), and Ann Marie Ascough (formerly contracted at the USGS - St. Petersburg, Fla.) helped with the creation of KML files. To view the photos and survey maps, proceed to the Photos and Maps page.

Sound source localization identification accuracy: Envelope dependencies.

PubMed

Yost, William A

2017-07-01

Sound source localization accuracy as measured in an identification procedure in a front azimuth sound field was studied for click trains, modulated noises, and a modulated tonal carrier. Sound source localization accuracy was determined as a function of the number of clicks in a 64 Hz click train and click rate for a 500 ms duration click train. The clicks were either broadband or high-pass filtered. Sound source localization accuracy was also measured for a single broadband filtered click and compared to a similar broadband filtered, short-duration noise. Sound source localization accuracy was determined as a function of sinusoidal amplitude modulation and the "transposed" process of modulation of filtered noises and a 4 kHz tone. Different rates (16 to 512 Hz) of modulation (including unmodulated conditions) were used. Providing modulation for filtered click stimuli, filtered noises, and the 4 kHz tone had, at most, a very small effect on sound source localization accuracy. These data suggest that amplitude modulation, while providing information about interaural time differences in headphone studies, does not have much influence on sound source localization accuracy in a sound field.

Cloning and characterization of luciferase from a Fijian luminous click beetle.

PubMed

Mitani, Yasuo; Futahashi, Ryo; Niwa, Kazuki; Ohba, Nobuyoshi; Ohmiya, Yoshihiro

2013-01-01

Luminous click beetle is distributed almost exclusively in Central and South America with a single genus in Melanesia. Among these click beetles, the description of Melanesian species has been fragmentary, and its luciferase gene and phylogenetic relation to other click beetles still remain uncertain. We collected a living luminous click beetle, Photophorus jansonii in Fiji. It emits green-yellow light from two spots on the pronotum and has no ventral luminous organ. Here, we cloned a luciferase gene from this insect by RT-PCR. The deduced amino acid sequence showed high identity of ~85% to the luciferases derived from other click beetle species. The luciferase of the Fijian click beetle was produced as a recombinant protein to characterize its biochemical properties. The Km for D-luciferin and ATP were 173 and 270 μm, respectively. The luciferase was pH-insensitive and the spectrum measured at pH 8.0 showed a peak at 559 nm, which was in the range of green-yellow light as seen in the luminous spot of the living Fijian click beetle. The Fijian click beetle luciferase was assigned to the Elateridae clade by a phylogenetic analysis, but it made a clearly different branch from Pyrophorus group examined in this study. © 2013 The American Society of Photobiology.

'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends.

PubMed

Mandhare, Anita; Banerjee, Paromita; Bhutkar, Smita; Hirwani, Rajkumar

2014-12-01

Click chemistry is the novel synthetic approach towards developing reactions with large thermodynamic driving forces to give almost complete conversion of new molecular reagents to a single product. Thus, click chemistry describes the chemistry for making carbon-heteroatom-carbon bonds in benign solvents, especially in water, and having a plethora of chemical and biological applications. This has played an important role in early detection of diseases, real-time monitoring of drug delivery and investigating the biomolecular functions in vivo. This review aims at highlighting the research advancements in click chemistry published in the patent literature and categorizing the patents according to the technological progress. An extensive search was carried out to collect and analyze the patent information claiming the use of click chemistry in biotechnology, especially for diagnosis. The study further concentrates on licensing of the click chemistry patents and defining the recent breakthroughs. Different databases like Espacenet, ISI Web of Science, Patbase and Thomson Innovation are used to compile the relevant literature. In recent years, considerable development in the click concept has encouraged researchers in using click reactions in almost every branch of industry that uses chemistry. Click chemistry for chemical ligation has been immensely explored in the field of biotechnology especially for detection, diagnosis and therapeutics.

Comparison of echolocation clicks from geographically sympatric killer whales and long-finned pilot whales (L).

PubMed

Eskesen, Ida G; Wahlberg, Magnus; Simon, Malene; Larsen, Ole Næsbye

2011-07-01

The source characteristics of biosonar signals from sympatric killer whales and long-finned pilot whales in a Norwegian fjord were compared. A total of 137 pilot whale and more than 2000 killer whale echolocation clicks were recorded using a linear four-hydrophone array. Of these, 20 pilot whale clicks and 28 killer whale clicks were categorized as being recorded on-axis. The clicks of pilot whales had a mean apparent source level of 196 dB re 1 μPa pp and those of killer whales 203 dB re 1 μPa pp. The duration of pilot whale clicks was significantly shorter (23 μs, S.E.=1.3) and the centroid frequency significantly higher (55 kHz, S.E.=2.1) than killer whale clicks (duration: 41 μs, S.E.=2.6; centroid frequency: 32 kHz, S.E.=1.5). The rate of increase in the accumulated energy as a function of time also differed between clicks from the two species. The differences in duration, frequency, and energy distribution may have a potential to allow for the distinction between pilot and killer whale clicks when using automated detection routines for acoustic monitoring. © 2011 Acoustical Society of America

Leveraging Social Media to Promote Evidence-Based Continuing Medical Education.

PubMed

Flynn, Simone; Hebert, Paul; Korenstein, Deborah; Ryan, Mark; Jordan, William B; Keyhani, Salomeh

2017-01-01

New dissemination methods are needed to engage physicians in evidence-based continuing medical education (CME). To examine the effectiveness of social media in engaging physicians in non-industry-sponsored CME. We tested the effect of different media platforms (e-mail, Facebook, paid Facebook and Twitter), CME topics, and different "hooks" (e.g., Q&A, clinical pearl and best evidence) on driving clicks to a landing site featuring non-industry sponsored CME. We modelled the effects of social media platform, CME topic, and hook using negative binomial regression on clicks to a single landing site. We used clicks to landing site adjusted for exposure and message number to calculate rate ratios. To understand how physicians interact with CME content on social media, we also conducted interviews with 10 physicians. The National Physicians Alliance (NPA) membership. NPA e-mail recipients, Facebook followers and friends, and Twitter followers. Clicks to the NPA's CME landing site. On average, 4,544 recipients received each message. Messages generated a total of 592 clicks to the landing site, for a rate of 5.4 clicks per 1000 recipients exposed. There were 5.4 clicks from e-mail, 11.9 clicks from Facebook, 5.5 clicks from paid Facebook, and 6.9 clicks from Twitter to the landing site for 1000 physicians exposed to each of 4 selected CME modules. A Facebook post generated 2.3x as many clicks to the landing site as did an e-mail after controlling for participant exposure, hook type and CME topic (p<0.001). Twitter posts (p = 0.13) and paid Facebook posts (p = 0.06) were not statistically different from e-mail in generating clicks to the landing site. Use of different hooks to engage physicians had no impact on clicks to the landing site. Interviews with physicians suggested that social media might not be a preferred vehicle for disseminating CME. Social media has a modest impact on driving traffic to evidence-based CME options. Facebook had a superior effect on driving physician web traffic to evidence-based CME compared to other social media platforms and email.

Leveraging Social Media to Promote Evidence-Based Continuing Medical Education

PubMed Central

Flynn, Simone; Hebert, Paul; Korenstein, Deborah; Ryan, Mark; Jordan, William B.

2017-01-01

Importance New dissemination methods are needed to engage physicians in evidence-based continuing medical education (CME). Objective To examine the effectiveness of social media in engaging physicians in non-industry-sponsored CME. Design We tested the effect of different media platforms (e-mail, Facebook, paid Facebook and Twitter), CME topics, and different “hooks” (e.g., Q&A, clinical pearl and best evidence) on driving clicks to a landing site featuring non-industry sponsored CME. We modelled the effects of social media platform, CME topic, and hook using negative binomial regression on clicks to a single landing site. We used clicks to landing site adjusted for exposure and message number to calculate rate ratios. To understand how physicians interact with CME content on social media, we also conducted interviews with 10 physicians. Setting The National Physicians Alliance (NPA) membership. Participants NPA e-mail recipients, Facebook followers and friends, and Twitter followers. Main Outcomes and Measures Clicks to the NPA’s CME landing site. Results On average, 4,544 recipients received each message. Messages generated a total of 592 clicks to the landing site, for a rate of 5.4 clicks per 1000 recipients exposed. There were 5.4 clicks from e-mail, 11.9 clicks from Facebook, 5.5 clicks from paid Facebook, and 6.9 clicks from Twitter to the landing site for 1000 physicians exposed to each of 4 selected CME modules. A Facebook post generated 2.3x as many clicks to the landing site as did an e-mail after controlling for participant exposure, hook type and CME topic (p<0.001). Twitter posts (p = 0.13) and paid Facebook posts (p = 0.06) were not statistically different from e-mail in generating clicks to the landing site. Use of different hooks to engage physicians had no impact on clicks to the landing site. Interviews with physicians suggested that social media might not be a preferred vehicle for disseminating CME. Conclusions Social media has a modest impact on driving traffic to evidence-based CME options. Facebook had a superior effect on driving physician web traffic to evidence-based CME compared to other social media platforms and email. PMID:28060854

The combined effects of forward masking by noise and high click rate on monaural and binaural human auditory nerve and brainstem potentials.

PubMed

Pratt, Hillel; Polyakov, Andrey; Bleich, Naomi; Mittelman, Naomi

2004-07-01

To study effects of forward masking and rapid stimulation on human monaurally- and binaurally-evoked brainstem potentials and suggest their relation to synaptic fatigue and recovery and to neuronal action potential refractoriness. Auditory brainstem evoked potentials (ABEPs) were recorded from 12 normally- and symmetrically hearing adults, in response to each click (50 dB nHL, condensation and rarefaction) in a train of nine, with an inter-click interval of 11 ms, that followed a white noise burst of 100 ms duration (50 dB nHL). Sequences of white noise and click train were repeated at a rate of 2.89 s(-1). The interval between noise and first click in the train was 2, 11, 22, 44, 66 or 88 ms in different runs. ABEPs were averaged (8000 repetitions) using a dwell time of 25 micros/address/channel. The binaural interaction components (BICs) of ABEPs were derived and the single, centrally located equivalent dipoles of ABEP waves I and V and of the BIC major wave were estimated. The latencies of dipoles I and V of ABEP, their inter-dipole interval and the dipole magnitude of component V were significantly affected by the interval between noise and clicks and by the serial position of the click in the train. The latency and dipole magnitude of the major BIC component were significantly affected by the interval between noise and clicks. Interval from noise and the click's serial position in the train interacted to affect dipole V latency, dipole V magnitude, BIC latencies and the V-I inter-dipole latency difference. Most of the effects were fully apparent by the first few clicks in the train, and the trend (increase or decrease) was affected by the interval between noise and clicks. The changes in latency and magnitude of ABEP and BIC components with advancing position in the click train and the interactions of click position in the train with the intervals from noise indicate an interaction of fatigue and recovery, compatible with synaptic depletion and replenishing, respectively. With the 2 ms interval between noise and the first click in the train, neuronal action potential refractoriness may also be involved.

Learning Clicks: Year End Report 2008/2009

ERIC Educational Resources Information Center

Alberta Advanced Education and Technology, 2009

2009-01-01

Learning Clicks was developed in 2003 as an interactive, fun way for Alberta students to learn about these opportunities. Learning Clicks is a program designed to support Strategy 2.4 in Alberta Advanced Education and Technology's 2007-10 Business Plan. The 2008/2009 season was the 5th year of the Learning Clicks program. This paper offers a…

Separation of Sperm Whale Click-Trains for Multipath Rejection and Localization

DTIC Science & Technology

2010-03-05

Correlation 12 3.3 Multipath Elimination Rules 13 4 LOCALIZATION 15 4.1 Localization Approach 15 4.2 Inter-Sensor Time-Delay Estimation Approach...Using Bayes’ rule , kj = ’°g kj = >°g< P(H, P(H0 HJ) \\J) (2) />(//,)p(zJ//,) p(H0)p(z,JH0) where p(//o) and p(H\\) are the a priori probabilities...overlapping clicks.) 3.3 MULTIPATH ELIMINATION RULES Multipath click-trains are eliminated if the individual clicks within the click-train are

Searching for Dual AGNs in Galaxy Mergers: Understanding Double-Peaked [O III] and Ultra Hard X-rays as Selection Method

NASA Astrophysics Data System (ADS)

McGurk, Rosalie C.; Max, Claire E.; Medling, Anne; Shields, Gregory A.

2015-01-01

When galaxies merge, gas accretes onto both central supermassive black holes. Thus, one expects to see close pairs of active galactic nuclei (AGNs), or dual AGNs, in a fraction of galaxy mergers. However, finding them remains a challenge. The presence of double-peaked [O III] or of ultra hard X-rays have been proposed as techniques to select dual AGNs efficiently. We studied a sample of double-peaked narrow [O III] emitting AGNs from SDSS DR7. By obtaining new and archival high spatial resolution images taken with the Keck 2 Laser Guide Star Adaptive Optics system and the near-infrared (IR) camera NIRC2, we showed that 30% of double-peaked [O III] emission line SDSS AGNs have two spatial components within a 3' radius. However, spatially resolved spectroscopy or X-ray observations are needed to confirm these galaxy pairs as systems containing two AGNs. We followed up these spatially-double candidate dual AGNs with integral field spectroscopy from Keck OSIRIS and Gemini GMOS and with long-slit spectroscopy from Keck NIRSPEC and Shane Kast Double Spectrograph. We find double-peaked emitters are caused sometimes by dual AGN and sometimes by outflows or narrow line kinematics. We also performed Chandra X-ray ACIS-S observations on 12 double-peaked candidate dual AGNs. Using our observations and 8 archival observations, we compare the distribution of X-ray photons to our spatially double near-IR images, measure X-ray luminosities and hardness ratios, and estimate column densities. By assessing what fraction of double-peaked emission line SDSS AGNs are true dual AGNs, we can better determine whether double-peaked [O III] is an efficient dual AGN indicator and constrain the statistics of dual AGNs. A second technique to find dual AGN is the detection of ultra hard X-rays by the Swift Burst Alert Telescope. We use CARMA observations to measure and map the CO(1-0) present in nearby ultra-hard X-ray Active Galactic Nuclei (AGNs) merging with either a quiescent companion galaxy or a companion galaxy hosting a second AGN, in order to understand the role molecular gas plays in feeding this unusual population of ultra-hard X-ray AGNs and to understand ultra-hard X-rays as a dual AGN selection method.

Modification of chemical and conformational properties of natural organic matter by click chemistry as revealed by ESI-Orbitrap mass spectrometry.

PubMed

Nebbioso, Antonio; Piccolo, Alessandro

2015-11-01

A click reaction is reported here for the first time as a useful technique to control the conformational stability of natural organic matter (NOM) suprastructures. Click conjugates were successfully formed between a previously butynylated NOM hydrophobic fraction and a hydrophilic polyethylene glycol (PEG)-amino chain. The click products were shown by size exclusion chromatography (HPSEC) hyphenated with Orbitrap mass spectrometry (MS) in electrospray ionization (ESI) (+), while precursors were visible in ESI (-). Despite their increase in molecular weight, HPSEC elution of click conjugates occurred after that of precursors, thus showing their departure from the NOM supramolecular association. This indicates that the click-conjugated NOM molecules were varied in their hydrophilic and cationic character and lost the capacity to accommodate in the original hydrophobic suprastructures. The most abundant product had the C16H30O5N4 formula, a click conjugate of butanoic acid, while other products were short-chained (C4-C8) linear unsaturated and hydroxylated carboxylic acids. Tandem MS revealed formation of triazole rings in clicked conjugates and their two fragmentations at the ester and the C-N alkyl-aryl bonds. The behavior of NOM molecules modified by click chemistry confirms that hydrophobicity and ionic charge of humic molecules play a pivotal role in stabilizing intermolecular forces in NOM. Moreover, the versatility of the click reaction may become useful to decorate NOM molecules with a variety of substrates, in order to alter NOM conformational and chemical properties and diversify its applications in the environment.

Genesis of the Mechanical Heart Valves' Ultrasonic Closing Clicks

NASA Astrophysics Data System (ADS)

Hasegawa, Jun; Kobayashi, Kenji

A new in vitro experimental tool was developed to study the mechanism of the ultrasonic closing clicks' genesis of mechanical heart valves. Since the newly developed tester adopted compressed air flow directly instead of the blood analog fluid to drive the mechanical heart valve, it is not possibe to generate any cavitation. Closing clicks were measured with a small accelerometer at the surface of the valve holder made of silicone rubber. Ultrasonic closing clicks as well as audible closing clicks, similar to those measured clinically, could be observed using this setup. Thus, it was confirmed that the ultrasonic closing clicks can be generated without the existence of cavitation. Simultaneous measurements of the valve motion were made with a high-speed video camera, and the analysis of the video frames and clicks showed that higher frequency signal components of more than 50kHz could be generated only at the instant of the closure, which means the collision of the occluder with the housing. Eighteen miniature accelerometers with an area of one square millimeter were developed and stuck on the housing to monitor the distribution of the housing vibrations in detail, and it was found that the vibrations correspond to the ultrasonic closing clicks propagated from the valve stop: the collision point of the occluder with the housing. This fact indicated that the generation of ultrasonic closing clicks are limited to the small area of the collision. From those results, it was concluded that the major origin of the ultrasonic closing clicks' genesis should be the collision of the occluder with the housing.

DNA with Parallel Strand Orientation: A Nanometer Distance Study with Spin Labels in the Watson-Crick and the Reverse Watson-Crick Double Helix.

PubMed

Wunnicke, Dorith; Ding, Ping; Yang, Haozhe; Seela, Frank; Steinhoff, Heinz-Jürgen

2015-10-29

Parallel-stranded (ps) DNA characterized by its sugar-phosphate backbones pointing in the same direction represents an alternative pairing system to antiparallel-stranded (aps) DNA with the potential to inhibit transcription and translation. 25-mer oligonucleotides were selected containing only dA·dT base pairs to compare spin-labeled nucleobase distances over a range of 10 or 15 base pairs in ps DNA with those in aps DNA. By means of the copper(I)-catalyzed Huisgen-Meldal-Sharpless alkyne-azide cycloaddition, the spin label 4-azido-2,2,6,6-tetramethylpiperidine-1-oxyl was clicked to 7-ethynyl-7-deaza-2'-deoxyadenosine or 5-ethynyl-2'-deoxyuridine to yield 25-mer oligonucleotides incorporating two spin labels. The interspin distances between spin labeled residues were determined by pulse EPR spectroscopy. The results reveal that in ps DNA these distances are between 5 and 10% longer than in aps DNA when the labeled DNA segment is located near the center of the double helix. The interspin distance in ps DNA becomes shorter compared with aps DNA when one of the spin labels occupies a position near the end of the double helix.

Double quick, double click reversible peptide "stapling".

PubMed

Grison, Claire M; Burslem, George M; Miles, Jennifer A; Pilsl, Ludwig K A; Yeo, David J; Imani, Zeynab; Warriner, Stuart L; Webb, Michael E; Wilson, Andrew J

2017-07-01

The development of constrained peptides for inhibition of protein-protein interactions is an emerging strategy in chemical biology and drug discovery. This manuscript introduces a versatile, rapid and reversible approach to constrain peptides in a bioactive helical conformation using BID and RNase S peptides as models. Dibromomaleimide is used to constrain BID and RNase S peptide sequence variants bearing cysteine (Cys) or homocysteine ( h Cys) amino acids spaced at i and i + 4 positions by double substitution. The constraint can be readily removed by displacement of the maleimide using excess thiol. This new constraining methodology results in enhanced α-helical conformation (BID and RNase S peptide) as demonstrated by circular dichroism and molecular dynamics simulations, resistance to proteolysis (BID) as demonstrated by trypsin proteolysis experiments and retained or enhanced potency of inhibition for Bcl-2 family protein-protein interactions (BID), or greater capability to restore the hydrolytic activity of the RNAse S protein (RNase S peptide). Finally, use of a dibromomaleimide functionalized with an alkyne permits further divergent functionalization through alkyne-azide cycloaddition chemistry on the constrained peptide with fluorescein, oligoethylene glycol or biotin groups to facilitate biophysical and cellular analyses. Hence this methodology may extend the scope and accessibility of peptide stapling.

The Click and Twitch in Contemporary Poetry.

ERIC Educational Resources Information Center

Meredith, Bernard

It is the creative writing instructor's role to help the student turn "twitch" poems into "click" poems ("twitch" being a kind of verbal hypertension that takes shape in the absence of anything humanly important to say on the poet's part and "click" being the finished poem that makes a sound like the click of the lid on a perfectly made box).…

Dolphin "packet" use during long-range echolocation tasks.

PubMed

Finneran, James J

2013-03-01

When echolocating, dolphins typically emit a single broadband "click," then wait to receive the echo before emitting another click. However, previous studies have shown that during long-range echolocation tasks, they may instead emit a burst, or "packet," of several clicks, then wait for the packet of echoes to return before emitting another packet of clicks. The reasons for the use of packets are unknown. In this study, packet use was examined by having trained bottlenose dolphins perform long-range echolocation tasks. The tasks featured "phantom" echoes produced by capturing the dolphin's outgoing echolocation clicks, convolving the clicks with an impulse response to create an echo waveform, and then broadcasting the delayed, scaled echo to the dolphin. Dolphins were trained to report the presence of phantom echoes or a change in phantom echoes. Target range varied from 25 to 800 m. At ranges below 75 m, the dolphins rarely used packets. As the range increased beyond 75 m, two of the three dolphins increasingly produced packets, while the third dolphin instead utilized very high click repetition rates. The use of click packets appeared to be governed more by echo delay (target range) than echo amplitude.

Comparisons of transient evoked otoacoustic emissions using chirp and click stimuli

PubMed Central

Keefe, Douglas H.; Feeney, M. Patrick; Hunter, Lisa L.; Fitzpatrick, Denis F.

2016-01-01

Transient-evoked otoacoustic emission (TEOAE) responses (0.7–8 kHz) were measured in normal-hearing adult ears using click stimuli and chirps whose local frequency increased or decreased linearly with time over the stimulus duration. Chirp stimuli were created by allpass filtering a click with relatively constant incident pressure level over frequency. Chirp TEOAEs were analyzed as a nonlinear residual signal by inverse allpass filtering each chirp response into an equivalent click response. Multi-window spectral and temporal averaging reduced noise levels compared to a single-window average. Mean TEOAE levels using click and chirp stimuli were similar with respect to their standard errors in adult ears. TEOAE group delay, group spread, instantaneous frequency, and instantaneous bandwidth were similar overall for chirp and click conditions, except for small differences showing nonlinear interactions differing across stimulus conditions. These results support the theory of a similar generation mechanism on the basilar membrane for both click and chirp conditions based on coherent reflection within the tonotopic region. TEOAE temporal fine structure was invariant across changes in stimulus level, which is analogous to the intensity invariance of click-evoked basilar-membrane displacement data. PMID:27914441

Target distance-dependent variation of hearing sensitivity during echolocation in a false killer whale.

PubMed

Supin, Alexander Ya; Nachtigall, Paul E; Breese, Marlee

2010-06-01

Evidence of varying hearing sensitivity according to the target distance was obtained in a false killer whale Pseudorca crassidens during echolocation. Auditory evoked potentials (AEPs) triggered by echolocation clicks were recorded. The target distance varied from 1 to 6 m. The records contained AEPs to the self-heard emitted click and AEPs to the echoes. Mean level of echolocation clicks depended on distance (the longer the distance, the higher the click level), however, the effect of click level on AEP amplitude was eliminated by extracting AEPs to clicks of certain particular levels. The amplitude of the echo-provoked AEP was almost independent of distance, however, the amplitude of the AEP to the emitted click, did depend on distance within a range from 1 to 4 m: the longer the distance, the higher the amplitude. The latter result is interpreted as confirmational evidence that the animal is capable of varying hearing sensitivity according to target distance. The variation of hearing sensitivity may help to compensate for the echo attenuation with distance; as a secondary effect, this variation manifested itself in a variation of the amplitude of the AEP to emitted clicks.

Non-auditory, electrophysiological potentials preceding dolphin biosonar click production.

PubMed

Finneran, James J; Mulsow, Jason; Jones, Ryan; Houser, Dorian S; Accomando, Alyssa W; Ridgway, Sam H

2018-03-01

The auditory brainstem response to a dolphin's own emitted biosonar click can be measured by averaging epochs of the instantaneous electroencephalogram (EEG) that are time-locked to the emitted click. In this study, averaged EEGs were measured using surface electrodes placed on the head in six different configurations while dolphins performed an echolocation task. Simultaneously, biosonar click emissions were measured using contact hydrophones on the melon and a hydrophone in the farfield. The averaged EEGs revealed an electrophysiological potential (the pre-auditory wave, PAW) that preceded the production of each biosonar click. The largest PAW amplitudes occurred with the non-inverting electrode just right of the midline-the apparent side of biosonar click generation-and posterior of the blowhole. Although the source of the PAW is unknown, the temporal and spatial properties rule out an auditory source. The PAW may be a neural or myogenic potential associated with click production; however, it is not known if muscles within the dolphin nasal system can be actuated at the high rates reported for dolphin click production, or if sufficiently coordinated and fast motor endplates of nasal muscles exist to produce a PAW detectable with surface electrodes.

Recording and quantification of ultrasonic echolocation clicks from free-ranging toothed whales

NASA Astrophysics Data System (ADS)

Madsen, P. T.; Wahlberg, M.

2007-08-01

Toothed whales produce short, ultrasonic clicks of high directionality and source level to probe their environment acoustically. This process, termed echolocation, is to a large part governed by the properties of the emitted clicks. Therefore derivation of click source parameters from free-ranging animals is of increasing importance to understand both how toothed whales use echolocation in the wild and how they may be monitored acoustically. This paper addresses how source parameters can be derived from free-ranging toothed whales in the wild using calibrated multi-hydrophone arrays and digital recorders. We outline the properties required of hydrophones, amplifiers and analog to digital converters, and discuss the problems of recording echolocation clicks on the axis of a directional sound beam. For accurate localization the hydrophone array apertures must be adapted and scaled to the behavior of, and the range to, the clicking animal, and precise information on hydrophone locations is critical. We provide examples of localization routines and outline sources of error that lead to uncertainties in localizing clicking animals in time and space. Furthermore we explore approaches to time series analysis of discrete versions of toothed whale clicks that are meaningful in a biosonar context.

Comparisons of transient evoked otoacoustic emissions using chirp and click stimuli.

PubMed

Keefe, Douglas H; Feeney, M Patrick; Hunter, Lisa L; Fitzpatrick, Denis F

2016-09-01

Transient-evoked otoacoustic emission (TEOAE) responses (0.7-8 kHz) were measured in normal-hearing adult ears using click stimuli and chirps whose local frequency increased or decreased linearly with time over the stimulus duration. Chirp stimuli were created by allpass filtering a click with relatively constant incident pressure level over frequency. Chirp TEOAEs were analyzed as a nonlinear residual signal by inverse allpass filtering each chirp response into an equivalent click response. Multi-window spectral and temporal averaging reduced noise levels compared to a single-window average. Mean TEOAE levels using click and chirp stimuli were similar with respect to their standard errors in adult ears. TEOAE group delay, group spread, instantaneous frequency, and instantaneous bandwidth were similar overall for chirp and click conditions, except for small differences showing nonlinear interactions differing across stimulus conditions. These results support the theory of a similar generation mechanism on the basilar membrane for both click and chirp conditions based on coherent reflection within the tonotopic region. TEOAE temporal fine structure was invariant across changes in stimulus level, which is analogous to the intensity invariance of click-evoked basilar-membrane displacement data.

Spectrogram analysis of low to mid frequency marine mammal clicks

NASA Astrophysics Data System (ADS)

Ioup, George E.; Ioup, Juliette W.; Larue, James P.; Sidorovskaia, Natalia A.; Kuczaj, Stan A.; Rayborn, Grayson H.; Walker, Christopher D.

2004-05-01

Previous investigators have proposed explanations for some sperm whale click structure and pointed out that the separation of individual pulses within the click might be used to determine approximately the size of the sperm whales. Recently, Mohl et al. [J. Acoust. Soc. Am. 114, 1124-1154 (2003)] have shown that echo-location click structure is highly dependent on the received angle. In data measured by the Littoral Acoustic Demonstration Center using bottom-moored hydrophones in the northern Gulf of Mexico in the summers of 2001 and 2002, rich click structures were observed in the spectrograms of many click trains, some of which exhibit strikingly consistent spectral nulls across the train. Although this structure in the spectra could be due to propagation effects, investigations to date suggest this possibility is highly unlikely, as discussed in the next abstract. Therefore it is at least plausible that the structure could be used to identify individual animals. This is known to be a difficult problem in the case of sperm whales because of the angle dependence of at least some of their clicks. These difficulties are discussed, as is the possible use of the spectrograms of the clicks to identify individuals. [Research supported by ONR.

Cu-free 1,3-dipolar cycloaddition click reactions to form isoxazole linkers in chelating ligands for fac-[M(I)(CO)3]+ centers (M = Re, 99mTc).

PubMed

Bottorff, Shalina C; Kasten, Benjamin B; Stojakovic, Jelena; Moore, Adam L; MacGillivray, Leonard R; Benny, Paul D

2014-02-17

Isoxazole ring formation was examined as a potential Cu-free alternative click reaction to Cu(I)-catalyzed alkyne/azide cycloaddition. The isoxazole reaction was explored at macroscopic and radiotracer concentrations with the fac-[M(I)(CO)3](+) (M = Re, (99m)Tc) core for use as a noncoordinating linker strategy between covalently linked molecules. Two click assembly methods (click, then chelate and chelate, then click) were examined to determine the feasibility of isoxazole ring formation with either alkyne-functionalized tridentate chelates or their respective fac-[M(I)(CO)3](+) complexes with a model nitrile oxide generator. Macroscale experiments, alkyne-functionalized chelates, or Re complexes indicate facile formation of the isoxazole ring. (99m)Tc experiments demonstrate efficient radiolabeling with click, then chelate; however, the chelate, then click approach led to faster product formation, but lower yields compared to the Re analogues.

28. Photocopy of drawing, July 30, 1952 (original in files ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

28. Photocopy of drawing, July 30, 1952 (original in files of United States Military Academy Architectural Archive, West Point, New York) NEW DOUBLE DECK PLATFORM AT ICE HOUSE BUILDING - U.S. Military Academy, Ice House, Mills Road at Howze Place, West Point, Orange County, NY

Early Restoration Projects Atlas | NOAA Gulf Spill Restoration

Science.gov Websites

trustees are implementing. To view details of an individual project, click the View icon on the list below or click the project marker on the map. For definitions of the project detail click here. To highlight the location of a project from the list, click the Show on Map icon. This atlas will be updated as

What speeds up the internal clock? Effects of clicks and flicker on duration judgements and reaction time.

PubMed

Wearden, J H; Williams, Emily A; Jones, Luke A

2017-03-01

Four experiments investigated the effect of pre-stimulus events on judgements of the subjective duration of tones that they preceded. Experiments 1 to 4 used click trains, flickering squares, expanding circles, and white noise as pre-stimulus events and showed that (a) periodic clicks appeared to "speed up" the pacemaker of an internal clock but that the effect wore off over a click-free delay, (b) aperiodic click trains, and visual stimuli in the form of flickering squares and expanding circles, also produced similar increases in estimated tone duration, as did white noise, although its effect was weaker. A fifth experiment examined the effects of periodic flicker on reaction time and showed that, as with periodic clicks in a previous experiment, reaction times were shorter when preceded by flicker than without.

Learning of Multimodal Representations With Random Walks on the Click Graph.

PubMed

Wu, Fei; Lu, Xinyan; Song, Jun; Yan, Shuicheng; Zhang, Zhongfei Mark; Rui, Yong; Zhuang, Yueting

2016-02-01

In multimedia information retrieval, most classic approaches tend to represent different modalities of media in the same feature space. With the click data collected from the users' searching behavior, existing approaches take either one-to-one paired data (text-image pairs) or ranking examples (text-query-image and/or image-query-text ranking lists) as training examples, which do not make full use of the click data, particularly the implicit connections among the data objects. In this paper, we treat the click data as a large click graph, in which vertices are images/text queries and edges indicate the clicks between an image and a query. We consider learning a multimodal representation from the perspective of encoding the explicit/implicit relevance relationship between the vertices in the click graph. By minimizing both the truncated random walk loss as well as the distance between the learned representation of vertices and their corresponding deep neural network output, the proposed model which is named multimodal random walk neural network (MRW-NN) can be applied to not only learn robust representation of the existing multimodal data in the click graph, but also deal with the unseen queries and images to support cross-modal retrieval. We evaluate the latent representation learned by MRW-NN on a public large-scale click log data set Clickture and further show that MRW-NN achieves much better cross-modal retrieval performance on the unseen queries/images than the other state-of-the-art methods.

Interaction of emitted sonar pulses and simulated echoes in a false killer whale: an evoked-potential study.

PubMed

Supin, Alexander Ya; Nachtigall, Paul E; Breese, Marlee

2011-09-01

Auditory evoked potentials (AEP) were recorded during echolocation in a false killer whale Pseudorca crassidens. An electronically synthesized and played-back (simulated) echo was triggered by an emitted biosonar pulse, and its intensity was proportional to that of the emitted click. The delay and transfer factor of the echo relative to the emitted click was controlled by the operator. The echo delay varied from 2 to 16 ms (by two-fold steps), and the transfer factor varied within ranges from -45 to -30 dB at the 2-ms delay to -60 to -45 dB at the 16-ms delay. Echo-related AEPs featured amplitude dependence both on echo delay at a constant transfer factor (the longer the delay, the higher amplitude) and on echo transfer factor at a constant delay (the higher transfer factor, the higher amplitude). Conjunctional variation of the echo transfer factor and delay kept the AEP amplitude constant when the delay to transfer factor trade was from -7.1 to -8.4 dB per delay doubling. The results confirm the hypothesis that partial forward masking of the echoes by the preceding emitted sonar pulses serves as a time-varying automatic gain control in the auditory system of echolocating odontocetes. © 2011 Acoustical Society of America

Auditory Function in Rhesus Monkeys: Effects of Aging and Caloric Restriction in the Wisconsin Monkeys Five Years Later

PubMed Central

Fowler, Cynthia G.; Chiasson, Kirstin Beach; Leslie, Tami Hanson; Thomas, Denise; Beasley, T. Mark; Kemnitz, Joseph W.; Weindruch, Richard

2010-01-01

Caloric restriction (CR) slows aging in many species and protects some animals from age-related hearing loss (ARHL), but the effect on humans is not yet known. Because rhesus monkeys are long-lived primates that are phylogenically closer to humans than other research animals are, they provide a better model for studying the effects of CR in aging and ARHL. Subjects were from the pool of 55 rhesus monkeys aged 15–28 years who had been in the Wisconsin study on CR and aging for 8–13.5 years. Distortion product otoacoustic emissions (DPOAE) with f2 frequencies from 2211–8837 Hz and auditory brainstem response (ABR) thresholds from clicks and 8, 16, and 32 kHz tone bursts were obtained. DPOAE levels declined linearly at approximately 1 dB/year, but that rate doubled for the highest frequencies in the oldest monkeys. There were no interactions for diet condition or sex. ABR thresholds to clicks and tone bursts showed increases with aging. Borderline significance was shown for diet in the thresholds at 8 kHz stimuli, with monkeys on caloric restriction having lower thresholds. Because the rhesus monkeys have a maximum longevity of 40 years, the full benefits of CR may not yet be realized. PMID:20079820

CDF-XL: computing cumulative distribution functions of reaction time data in Excel.

PubMed

Houghton, George; Grange, James A

2011-12-01

In experimental psychology, central tendencies of reaction time (RT) distributions are used to compare different experimental conditions. This emphasis on the central tendency ignores additional information that may be derived from the RT distribution itself. One method for analysing RT distributions is to construct cumulative distribution frequency plots (CDFs; Ratcliff, Psychological Bulletin 86:446-461, 1979). However, this method is difficult to implement in widely available software, severely restricting its use. In this report, we present an Excel-based program, CDF-XL, for constructing and analysing CDFs, with the aim of making such techniques more readily accessible to researchers, including students (CDF-XL can be downloaded free of charge from the Psychonomic Society's online archive). CDF-XL functions as an Excel workbook and starts from the raw experimental data, organised into three columns (Subject, Condition, and RT) on an Input Data worksheet (a point-and-click utility is provided for achieving this format from a broader data set). No further preprocessing or sorting of the data is required. With one click of a button, CDF-XL will generate two forms of cumulative analysis: (1) "standard" CDFs, based on percentiles of participant RT distributions (by condition), and (2) a related analysis employing the participant means of rank-ordered RT bins. Both analyses involve partitioning the data in similar ways, but the first uses a "median"-type measure at the participant level, while the latter uses the mean. The results are presented in three formats: (i) by participants, suitable for entry into further statistical analysis; (ii) grand means by condition; and (iii) completed CDF plots in Excel charts.

Click-based echolocation in bats: not so primitive after all.

PubMed

Yovel, Yossi; Geva-Sagiv, Maya; Ulanovsky, Nachum

2011-05-01

Echolocating bats of the genus Rousettus produce click sonar signals, using their tongue (lingual echolocation). These signals are often considered rudimentary and are believed to enable only crude performance. However, the main argument supporting this belief, namely the click's reported long duration, was recently shown to be an artifact. In fact, the sonar clicks of Rousettus bats are extremely short, ~50-100 μs, similar to dolphin vocalizations. Here, we present a comparison between the sonar systems of the 'model species' of laryngeal echolocation, the big brown bat (Eptesicus fuscus), and that of lingual echolocation, the Egyptian fruit bat (Rousettus aegyptiacus). We show experimentally that in tasks, such as accurate landing or detection of medium-sized objects, click-based echolocation enables performance similar to laryngeal echolocators. Further, we describe a sophisticated behavioral strategy for biosonar beam steering in clicking bats. Finally, theoretical analyses of the signal design--focusing on their autocorrelations and wideband ambiguity functions--predict that in some aspects, such as target ranging and Doppler-tolerance, click-based echolocation might outperform laryngeal echolocation. Therefore, we suggest that click-based echolocation in bats should be regarded as a viable echolocation strategy, which is in fact similar to the biosonar used by most echolocating animals, including whales and dolphins.

Assessing segmentation processes by click detection: online measure of statistical learning, or simple interference?

PubMed

Franco, Ana; Gaillard, Vinciane; Cleeremans, Axel; Destrebecqz, Arnaud

2015-12-01

Statistical learning can be used to extract the words from continuous speech. Gómez, Bion, and Mehler (Language and Cognitive Processes, 26, 212-223, 2011) proposed an online measure of statistical learning: They superimposed auditory clicks on a continuous artificial speech stream made up of a random succession of trisyllabic nonwords. Participants were instructed to detect these clicks, which could be located either within or between words. The results showed that, over the length of exposure, reaction times (RTs) increased more for within-word than for between-word clicks. This result has been accounted for by means of statistical learning of the between-word boundaries. However, even though statistical learning occurs without an intention to learn, it nevertheless requires attentional resources. Therefore, this process could be affected by a concurrent task such as click detection. In the present study, we evaluated the extent to which the click detection task indeed reflects successful statistical learning. Our results suggest that the emergence of RT differences between within- and between-word click detection is neither systematic nor related to the successful segmentation of the artificial language. Therefore, instead of being an online measure of learning, the click detection task seems to interfere with the extraction of statistical regularities.

The energy ratio mapping algorithm: a tool to improve the energy-based detection of odontocete echolocation clicks.

PubMed

Klinck, Holger; Mellinger, David K

2011-04-01

The energy ratio mapping algorithm (ERMA) was developed to improve the performance of energy-based detection of odontocete echolocation clicks, especially for application in environments with limited computational power and energy such as acoustic gliders. ERMA systematically evaluates many frequency bands for energy ratio-based detection of echolocation clicks produced by a target species in the presence of the species mix in a given geographic area. To evaluate the performance of ERMA, a Teager-Kaiser energy operator was applied to the series of energy ratios as derived by ERMA. A noise-adaptive threshold was then applied to the Teager-Kaiser function to identify clicks in data sets. The method was tested for detecting clicks of Blainville's beaked whales while rejecting echolocation clicks of Risso's dolphins and pilot whales. Results showed that the ERMA-based detector correctly identified 81.6% of the beaked whale clicks in an extended evaluation data set. Average false-positive detection rate was 6.3% (3.4% for Risso's dolphins and 2.9% for pilot whales).

“Click, Click, Cyclize”: A DOS Approach to Sultams Utilizing Vinyl Sulfonamide Linchpins

PubMed Central

Zhou, Aihua; Rayabarapu, Dinesh; Hanson, Paul R.

2009-01-01

A diversity-oriented synthesis (DOS) strategy termed “Click, Click, Cyclize” is reported. This approach relies on functional group (FG) pairing between a vinyl sulfonamide and an array of functional groups to synthesize skeletally diverse sultams. Several FG pairing pathways on central tertiary vinyl sulfonamide linchpins have been developed including intramolecular Heck, aza-Michael, ring-closing enyne metathesis, Pauson—Khand, and chemoselective oxidation/Baylis—Hillman reactions. PMID:19115841

Click trains and the rate of information processing: does "speeding up" subjective time make other psychological processes run faster?

PubMed

Jones, Luke A; Allely, Clare S; Wearden, John H

2011-02-01

A series of experiments demonstrated that a 5-s train of clicks that have been shown in previous studies to increase the subjective duration of tones they precede (in a manner consistent with "speeding up" timing processes) could also have an effect on information-processing rate. Experiments used studies of simple and choice reaction time (Experiment 1), or mental arithmetic (Experiment 2). In general, preceding trials by clicks made response times significantly shorter than those for trials without clicks, but white noise had no effects on response times. Experiments 3 and 4 investigated the effects of clicks on performance on memory tasks, using variants of two classic experiments of cognitive psychology: Sperling's (1960) iconic memory task and Loftus, Johnson, and Shimamura's (1985) iconic masking task. In both experiments participants were able to recall or recognize significantly more information from stimuli preceded by clicks than those preceded by silence.

African Y chromosome and mtDNA divergence provides insight into the history of click languages.

PubMed

Knight, Alec; Underhill, Peter A; Mortensen, Holly M; Zhivotovsky, Lev A; Lin, Alice A; Henn, Brenna M; Louis, Dorothy; Ruhlen, Merritt; Mountain, Joanna L

2003-03-18

About 30 languages of southern Africa, spoken by Khwe and San, are characterized by a repertoire of click consonants and phonetic accompaniments. The Jumid R:'hoansi (!Kung) San carry multiple deeply coalescing gene lineages. The deep genetic diversity of the San parallels the diversity among the languages they speak. Intriguingly, the language of the Hadzabe of eastern Africa, although not closely related to any other language, shares click consonants and accompaniments with languages of Khwe and San. We present original Y chromosome and mtDNA variation of Hadzabe and other ethnic groups of Tanzania and Y chromosome variation of San and peoples of the central African forests: Biaka, Mbuti, and Lisongo. In the context of comparable published data for other African populations, analyses of each of these independently inherited DNA segments indicate that click-speaking Hadzabe and Jumid R:'hoansi are separated by genetic distance as great or greater than that between any other pair of African populations. Phylogenetic tree topology indicates a basal separation of the ancient ancestors of these click-speaking peoples. That genetic divergence does not appear to be the result of recent gene flow from neighboring groups. The deep genetic divergence among click-speaking peoples of Africa and mounting linguistic evidence suggest that click consonants date to early in the history of modern humans. At least two explanations remain viable. Clicks may have persisted for tens of thousands of years, independently in multiple populations, as a neutral trait. Alternatively, clicks may have been retained, because they confer an advantage during hunting in certain environments.

People's Ability to Detect Objects Using Click-Based Echolocation: A Direct Comparison between Mouth-Clicks and Clicks Made by a Loudspeaker.

PubMed

Thaler, Lore; Castillo-Serrano, Josefina

2016-01-01

Echolocation is the ability to use reflected sound to obtain information about the spatial environment. Echolocation is an active process that requires both the production of the emission as well as the sensory processing of the resultant sound. Appreciating the general usefulness of echo-acoustic cues for people, in particular those with vision impairments, various devices have been built that exploit the principle of echolocation to obtain and provide information about the environment. It is common to all these devices that they do not require the person to make a sound. Instead, the device produces the emission autonomously and feeds a resultant sound back to the user. Here we tested if echolocation performance in a simple object detection task was affected by the use of a head-mounted loudspeaker as compared to active clicking. We found that 27 sighted participants new to echolocation did generally better when they used a loudspeaker as compared to mouth-clicks, and that two blind participants with experience in echolocation did equally well with mouth clicks and the speaker. Importantly, performance of sighted participants' was not statistically different from performance of blind experts when they used the speaker. Based on acoustic click data collected from a subset of our participants, those participants whose mouth clicks were more similar to the speaker clicks, and thus had higher peak frequencies and sound intensity, did better. We conclude that our results are encouraging for the consideration and development of assistive devices that exploit the principle of echolocation.

People’s Ability to Detect Objects Using Click-Based Echolocation: A Direct Comparison between Mouth-Clicks and Clicks Made by a Loudspeaker

PubMed Central

Thaler, Lore; Castillo-Serrano, Josefina

2016-01-01

Echolocation is the ability to use reflected sound to obtain information about the spatial environment. Echolocation is an active process that requires both the production of the emission as well as the sensory processing of the resultant sound. Appreciating the general usefulness of echo-acoustic cues for people, in particular those with vision impairments, various devices have been built that exploit the principle of echolocation to obtain and provide information about the environment. It is common to all these devices that they do not require the person to make a sound. Instead, the device produces the emission autonomously and feeds a resultant sound back to the user. Here we tested if echolocation performance in a simple object detection task was affected by the use of a head-mounted loudspeaker as compared to active clicking. We found that 27 sighted participants new to echolocation did generally better when they used a loudspeaker as compared to mouth-clicks, and that two blind participants with experience in echolocation did equally well with mouth clicks and the speaker. Importantly, performance of sighted participants’ was not statistically different from performance of blind experts when they used the speaker. Based on acoustic click data collected from a subset of our participants, those participants whose mouth clicks were more similar to the speaker clicks, and thus had higher peak frequencies and sound intensity, did better. We conclude that our results are encouraging for the consideration and development of assistive devices that exploit the principle of echolocation. PMID:27135407

Design of the biosonar simulator for dolphin's clicks waveform reproduction

NASA Astrophysics Data System (ADS)

Ishii, Ken; Akamatsu, Tomonari; Hatakeyama, Yoshimi

1992-03-01

The emitted clicks of Dall's porpoises consist of a pulse train of burst signals with an ultrasonic carrier frequency. The authors have designed a biosonar simulator to reproduce the waveforms associated with a dolphin's clicks underwater. The total reproduction system consists of a click signal acquisition block, a waveform analysis block, a memory unit, a click simulator, and a underwater, ultrasonic wave transmitter. In operation, data stored in an EPROM (Erasable Programmable Read Only Memory) are read out sequentially by a fast clock and converted to analog output signals. Then an ultrasonic power amplifier reproduces these signals through a transmitter. The click signal replaying block is referred to as the BSS (Biosonar Simulator). This is what simulates the clicks. The details of the BSS are described in this report. A unit waveform is defined. The waveform is divided into a burst period and a waiting period. Clicks are a sequence based on a unit waveform, and digital data are sequentially read out from an EPROM of waveform data. The basic parameters of the BSS are as follows: (1) reading clock, 100 ns to 25.4 microseconds; (2) number of reading clock, 34 to 1024 times; (3) counter clock in a waiting period, 100 ns to 25.4 microseconds; (4) number of counter clock, zero to 16,777,215 times; (5) number of burst/waiting repetition cycle, one to 128 times; and (6) transmission level adjustment by a programmable attenuator, zero to 86.5 dB. These basic functions enable the BSS to replay clicks of Dall's porpoise precisely.

Towards high conductivity in anion-exchange membranes for alkaline fuel cells.

PubMed

Li, Nanwen; Guiver, Michael D; Binder, Wolfgang H

2013-08-01

Quaternized poly(2,6-dimethylphenylene oxide) materials (PPOs) containing clicked 1,2,3-triazoles were first prepared through Cu(I) -catalyzed "click chemistry" to improve the anion transport in anion-exchange membranes (AEMs). Clicked 1,2,3-triazoles incorporated into AEMs provided more sites to form efficient and continuous hydrogen-bond networks between the water/hydroxide and the triazole for anion transport. Higher water uptake was observed for these triazole membranes. Thus, the membranes showed an impressive enhancement of the hydroxide diffusion coefficient and, therefore, the anion conductivities. The recorded hydroxide conductivity was 27.8-62 mS cm(-1) at 20 °C in water, which was several times higher than that of a typical PPO-based AEM (TMA-20) derived from trimethylamine (5 mS cm(-1) ). Even at reduced relative humidity, the clicked membrane showed superior conductivity to a trimethylamine-based membrane. Moreover, similar alkaline stabilities at 80 °C in 1 M NaOH were observed for the clicked and non-clicked membranes. The performance of a H2 /O2 single cell assembled with a clicked AEM was much improved compared to that of a non-clicked TMA-20 membrane. The peak power density achieved for an alkaline fuel cell with the synthesized membrane 1a(20) was 188.7 mW cm(-2) at 50 °C. These results indicated that clicked AEM could be a viable strategy for improving the performance of alkaline fuel cells. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

U.S. Army Concept of Operations and Standard Operating Procedure for Acquisition Program Managers Using Item Unique Identification

DTIC Science & Technology

2017-09-01

Figure 58. Click on run ................................................................................................61 Figure 59. Top view of...engines, helicopter rotors, and turbine blades , and so forth Creating Marks Readable with a Scanner 4.  Simple techniques to follow:  Make the light...spreadsheet with data Figure 58. Click on Menu bar and find “View” then click on “Macros.” Click on run Figure 59. 62 Top view of xml spreadsheet

Clicking in a Killer Whale Habitat: Narrow-Band, High-Frequency Biosonar Clicks of Harbour Porpoise (Phocoena phocoena) and Dall’s Porpoise (Phocoenoides dalli)

PubMed Central

Kyhn, Line A.; Tougaard, Jakob; Beedholm, Kristian; Jensen, Frants H.; Ashe, Erin; Williams, Rob; Madsen, Peter T.

2013-01-01

Odontocetes produce a range of different echolocation clicks but four groups in different families have converged on producing the same stereotyped narrow band high frequency (NBHF) click. In microchiropteran bats, sympatric species have evolved the use of different acoustic niches and subtly different echolocation signals to avoid competition among species. In this study, we examined whether similar adaptations are at play among sympatric porpoise species that use NBHF echolocation clicks. We used a six-element hydrophone array to record harbour and Dall’s porpoises in British Columbia (BC), Canada, and harbour porpoises in Denmark. The click source properties of all porpoise groups were remarkably similar and had an average directivity index of 25 dB. Yet there was a small, but consistent and significant 4 kHz difference in centroid frequency between sympatric Dall’s (137±3 kHz) and Canadian harbour porpoises (141±2 kHz). Danish harbour porpoise clicks (136±3 kHz) were more similar to Dall’s porpoise than to their conspecifics in Canada. We suggest that the spectral differences in echolocation clicks between the sympatric porpoises are consistent with evolution of a prezygotic isolating barrier (i.e., character displacement) to avoid hybridization of sympatric species. In practical terms, these spectral differences have immediate application to passive acoustic monitoring. PMID:23723996

Clicking in a killer whale habitat: narrow-band, high-frequency biosonar clicks of harbour porpoise (Phocoena phocoena) and Dall's porpoise (Phocoenoides dalli).

PubMed

Kyhn, Line A; Tougaard, Jakob; Beedholm, Kristian; Jensen, Frants H; Ashe, Erin; Williams, Rob; Madsen, Peter T

2013-01-01

Odontocetes produce a range of different echolocation clicks but four groups in different families have converged on producing the same stereotyped narrow band high frequency (NBHF) click. In microchiropteran bats, sympatric species have evolved the use of different acoustic niches and subtly different echolocation signals to avoid competition among species. In this study, we examined whether similar adaptations are at play among sympatric porpoise species that use NBHF echolocation clicks. We used a six-element hydrophone array to record harbour and Dall's porpoises in British Columbia (BC), Canada, and harbour porpoises in Denmark. The click source properties of all porpoise groups were remarkably similar and had an average directivity index of 25 dB. Yet there was a small, but consistent and significant 4 kHz difference in centroid frequency between sympatric Dall's (137±3 kHz) and Canadian harbour porpoises (141±2 kHz). Danish harbour porpoise clicks (136±3 kHz) were more similar to Dall's porpoise than to their conspecifics in Canada. We suggest that the spectral differences in echolocation clicks between the sympatric porpoises are consistent with evolution of a prezygotic isolating barrier (i.e., character displacement) to avoid hybridization of sympatric species. In practical terms, these spectral differences have immediate application to passive acoustic monitoring.

Click train encoding in primary and non-primary auditory cortex of anesthetized macaque monkeys.

PubMed

Oshurkova, E; Scheich, H; Brosch, M

2008-06-02

We studied encoding of temporally modulated sounds in 28 multiunits in the primary auditory cortical field (AI) and in 35 multiunits in the secondary auditory cortical field (caudomedial auditory cortical field, CM) by presenting periodic click trains with click rates between 1 and 300 Hz lasting for 2-4 s. We found that all multiunits increased or decreased their firing rate during the steady state portion of the click train and that all except two multiunits synchronized their firing to individual clicks in the train. Rate increases and synchronized responses were most prevalent and strongest at low click rates, as expressed by best modulation frequency, limiting frequency, percentage of responsive multiunits, and average rate response and vector strength. Synchronized responses occurred up to 100 Hz; rate response occurred up to 300 Hz. Both auditory fields responded similarly to low click rates but differed at click rates above approximately 12 Hz at which more multiunits in AI than in CM exhibited synchronized responses and increased rate responses and more multiunits in CM exhibited decreased rate responses. These findings suggest that the auditory cortex of macaque monkeys encodes temporally modulated sounds similar to the auditory cortex of other mammals. Together with other observations presented in this and other reports, our findings also suggest that AI and CM have largely overlapping sensitivities for acoustic stimulus features but encode these features differently.

Click-Evoked Auditory Efferent Activity: Rate and Level Effects.

PubMed

Boothalingam, Sriram; Kurke, Julianne; Dhar, Sumitrajit

2018-05-07

There currently are no standardized protocols to evaluate auditory efferent function in humans. Typical tests use broadband noise to activate the efferents, but only test the contralateral efferent pathway, risk activating the middle ear muscle reflex (MEMR), and are laborious for clinical use. In an attempt to develop a clinical test of bilateral auditory efferent function, we have designed a method that uses clicks to evoke efferent activity, obtain click-evoked otoacoustic emissions (CEOAEs), and monitor MEMR. This allows for near-simultaneous estimation of cochlear and efferent function. In the present study, we manipulated click level (60, 70, and 80 dB peak-equivalent sound pressure level [peSPL]) and rate (40, 50, and 62.5 Hz) to identify an optimal rate-level combination that evokes measurable efferent modulation of CEOAEs. Our findings (n = 58) demonstrate that almost all click levels and rates used caused significant inhibition of CEOAEs, with a significant interaction between level and rate effects. Predictably, bilateral activation produced greater inhibition compared to stimulating the efferents only in the ipsilateral or contralateral ear. In examining the click rate-level effects during bilateral activation in greater detail, we observed a 1-dB inhibition of CEOAE level for each 10-dB increase in click level, with rate held constant at 62.5 Hz. Similarly, a 10-Hz increase in rate produced a 0.74-dB reduction in CEOAE level, with click level held constant at 80 dB peSPL. The effect size (Cohen's d) was small for either monaural condition and medium for bilateral, faster-rate, and higher-level conditions. We were also able to reliably extract CEOAEs from efferent eliciting clicks. We conclude that clicks can indeed be profitably employed to simultaneously evaluate cochlear health using CEOAEs as well as their efferent modulation. Furthermore, using bilateral clicks allows the evaluation of both the crossed and uncrossed elements of the auditory efferent nervous system, while yielding larger, more discernible, inhibition of the CEOAEs relative to either ipsilateral or contralateral condition.

Nonecholocating fruit bats produce biosonar clicks with their wings.

PubMed

Boonman, Arjan; Bumrungsri, Sara; Yovel, Yossi

2014-12-15

Because evolution mostly acts over millions of years, the intermediate steps leading to a functional sensory system remain enigmatic. Accordingly, there is an ongoing debate regarding the evolution of bat echolocation. In search of the origin of bat echolocation, we studied how Old World fruit bats, which have always been classified as nonecholocating, orient in complete darkness. We found that two of these nonecholocating species used click-like sounds to detect and discriminate objects in complete darkness. However, we discovered that this click-based echo sensing is rudimentary and does not allow these bats to estimate distance accurately as all other echolocating bats can. Moreover, unlike all other echolocating bats, which generate pulses using the larynx or the tongue, these bats generated clicks with their wings. We provide evidence suggesting that all Old World fruit bats can click with their wings. Although this click-based echo sensing used by Old World fruit bats may not represent the ancestral form of current (laryngeal) bat echolocation, we argue that clicking fruit bats could be considered behavioral fossils, opening a window to study the evolution of echolocation. Copyright © 2014 Elsevier Ltd. All rights reserved.

Vocal reporting of echolocation targets: dolphins often report before click trains end.

PubMed

Ridgway, S H; Elsberry, W R; Blackwood, D J; Kamolnick, T; Todd, M; Carder, D A; Chaplin, Monica; Cranford, T W

2012-01-01

Bottlenose dolphins (Tursiops truncatus) wore opaque suction cups over their eyes while stationing behind an acoustically opaque door. This put the dolphins in a known position and orientation. When the door opened, the dolphin clicked to detect targets. Trainers specified that Dolphin S emit a whistle if the target was a 7.5 cm water filled sphere, or a pulse burst if the target was a rock. S remained quiet if there was no target. Dolphin B whistled for the sphere. She remained quiet for rock and for no target. Thus, S had to choose between three different responses, whistle, pulse burst, or remain quiet. B had to choose between two different responses, whistle or remain quiet. S gave correct vocal responses averaging 114 ms after her last echolocation click (range 182 ms before and 219 ms after the last click). Average response for B was 21 ms before her last echolocation click (range 250 ms before and 95 ms after the last click in the train). More often than not, B began her whistle response before her echolocation train ended. The findings suggest separate neural pathways for generation of response vocalizations as opposed to echolocation clicks. © 2012 Acoustical Society of America.

Learning to rank using user clicks and visual features for image retrieval.

PubMed

Yu, Jun; Tao, Dacheng; Wang, Meng; Rui, Yong

2015-04-01

The inconsistency between textual features and visual contents can cause poor image search results. To solve this problem, click features, which are more reliable than textual information in justifying the relevance between a query and clicked images, are adopted in image ranking model. However, the existing ranking model cannot integrate visual features, which are efficient in refining the click-based search results. In this paper, we propose a novel ranking model based on the learning to rank framework. Visual features and click features are simultaneously utilized to obtain the ranking model. Specifically, the proposed approach is based on large margin structured output learning and the visual consistency is integrated with the click features through a hypergraph regularizer term. In accordance with the fast alternating linearization method, we design a novel algorithm to optimize the objective function. This algorithm alternately minimizes two different approximations of the original objective function by keeping one function unchanged and linearizing the other. We conduct experiments on a large-scale dataset collected from the Microsoft Bing image search engine, and the results demonstrate that the proposed learning to rank models based on visual features and user clicks outperforms state-of-the-art algorithms.

Source parameter estimates of echolocation clicks from wild pygmy killer whales (Feresa attenuata) (L)

NASA Astrophysics Data System (ADS)

Madsen, P. T.; Kerr, I.; Payne, R.

2004-10-01

Pods of the little known pygmy killer whale (Feresa attenuata) in the northern Indian Ocean were recorded with a vertical hydrophone array connected to a digital recorder sampling at 320 kHz. Recorded clicks were directional, short (25 μs) transients with estimated source levels between 197 and 223 dB re. 1 μPa (pp). Spectra of clicks recorded close to or on the acoustic axis were bimodal with peak frequencies between 45 and 117 kHz, and with centroid frequencies between 70 and 85 kHz. The clicks share characteristics of echolocation clicks from similar sized, whistling delphinids, and have properties suited for the detection and classification of prey targeted by this odontocete. .

Kansas Department of Health and Environment - Immunization Program

Science.gov Websites

Immunize and Win a Prize Click on Immunize Win a Prize logo for more information Yellow Fever Click on the logo above for more information on Yellow Fever PERINATAL HEPATITIS B PREVENTION PROGRAM Click on logo

Ionic liquid syntheses via click chemistry: expeditious routes toward versatile functional materials.

PubMed

Mirjafari, Arsalan

2018-03-25

Since the introduction of click chemistry by K. B. Sharpless in 2001, its exploration and exploitation has occurred in countless fields of materials sciences in both academic and industrial spheres. Click chemistry is defined as an efficient, robust, and orthogonal synthetic platform for the facile formation of new carbon-heteroatom bonds, using readily available starting materials. Premier examples of click reactions are copper(i)-catalyzed azide-alkyne Huisgen cycloaddition (CuAAC) and the thiol-X (X = ene and yne) coupling reactions to form C-N and C-S bonds, respectively. The emphasis of this review is centered on the rapidly expanding area of click chemistry-mediated synthesis of functional ionic liquids via CuAAC, thiol-X and oxime formation, and selected examples of nucleophilic ring-opening reactions, while offering some thoughts on emerging challenges, opportunities and ultimately the evolution of this field. Click chemistry offers tremendous opportunities, and introduces intriguing perspectives for efficient and robust generation of tailored task-specific ionic liquids - an important class of soft materials.

MSLICE Sequencing

NASA Technical Reports Server (NTRS)

Crockett, Thomas M.; Joswig, Joseph C.; Shams, Khawaja S.; Norris, Jeffrey S.; Morris, John R.

2011-01-01

MSLICE Sequencing is a graphical tool for writing sequences and integrating them into RML files, as well as for producing SCMF files for uplink. When operated in a testbed environment, it also supports uplinking these SCMF files to the testbed via Chill. This software features a free-form textural sequence editor featuring syntax coloring, automatic content assistance (including command and argument completion proposals), complete with types, value ranges, unites, and descriptions from the command dictionary that appear as they are typed. The sequence editor also has a "field mode" that allows tabbing between arguments and displays type/range/units/description for each argument as it is edited. Color-coded error and warning annotations on problematic tokens are included, as well as indications of problems that are not visible in the current scroll range. "Quick Fix" suggestions are made for resolving problems, and all the features afforded by modern source editors are also included such as copy/cut/paste, undo/redo, and a sophisticated find-and-replace system optionally using regular expressions. The software offers a full XML editor for RML files, which features syntax coloring, content assistance and problem annotations as above. There is a form-based, "detail view" that allows structured editing of command arguments and sequence parameters when preferred. The "project view" shows the user s "workspace" as a tree of "resources" (projects, folders, and files) that can subsequently be opened in editors by double-clicking. Files can be added, deleted, dragged-dropped/copied-pasted between folders or projects, and these operations are undoable and redoable. A "problems view" contains a tabular list of all problems in the current workspace. Double-clicking on any row in the table opens an editor for the appropriate sequence, scrolling to the specific line with the problem, and highlighting the problematic characters. From there, one can invoke "quick fix" as described above to resolve the issue. Once resolved, saving the file causes the problem to be removed from the problem view.

High throughput imaging cytometer with acoustic focussing† †Electronic supplementary information (ESI) available: High throughput imaging cytometer with acoustic focussing. See DOI: 10.1039/c5ra19497k Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file.

PubMed Central

Zmijan, Robert; Jonnalagadda, Umesh S.; Carugo, Dario; Kochi, Yu; Lemm, Elizabeth; Packham, Graham; Hill, Martyn

2015-01-01

We demonstrate an imaging flow cytometer that uses acoustic levitation to assemble cells and other particles into a sheet structure. This technique enables a high resolution, low noise CMOS camera to capture images of thousands of cells with each frame. While ultrasonic focussing has previously been demonstrated for 1D cytometry systems, extending the technology to a planar, much higher throughput format and integrating imaging is non-trivial, and represents a significant jump forward in capability, leading to diagnostic possibilities not achievable with current systems. A galvo mirror is used to track the images of the moving cells permitting exposure times of 10 ms at frame rates of 50 fps with motion blur of only a few pixels. At 80 fps, we demonstrate a throughput of 208 000 beads per second. We investigate the factors affecting motion blur and throughput, and demonstrate the system with fluorescent beads, leukaemia cells and a chondrocyte cell line. Cells require more time to reach the acoustic focus than beads, resulting in lower throughputs; however a longer device would remove this constraint. PMID:29456838

A New Arbiter PUF for Enhancing Unpredictability on FPGA

PubMed Central

Machida, Takanori; Yamamoto, Dai; Iwamoto, Mitsugu; Sakiyama, Kazuo

2015-01-01

In general, conventional Arbiter-based Physically Unclonable Functions (PUFs) generate responses with low unpredictability. The N-XOR Arbiter PUF, proposed in 2007, is a well-known technique for improving this unpredictability. In this paper, we propose a novel design for Arbiter PUF, called Double Arbiter PUF, to enhance the unpredictability on field programmable gate arrays (FPGAs), and we compare our design to conventional N-XOR Arbiter PUFs. One metric for judging the unpredictability of responses is to measure their tolerance to machine-learning attacks. Although our previous work showed the superiority of Double Arbiter PUFs regarding unpredictability, its details were not clarified. We evaluate the dependency on the number of training samples for machine learning, and we discuss the reason why Double Arbiter PUFs are more tolerant than the N-XOR Arbiter PUFs by evaluating intrachip variation. Further, the conventional Arbiter PUFs and proposed Double Arbiter PUFs are evaluated according to other metrics, namely, their uniqueness, randomness, and steadiness. We demonstrate that 3-1 Double Arbiter PUF archives the best performance overall. PMID:26491720

Click it or ticket evaluation, 2008–2009 : traffic tech.

DOT National Transportation Integrated Search

2013-06-01

Click It or Ticket (CIOT) is a high-visibility enforcement : program to increase seat belt use. Click It or Ticket was : adopted as a national program in 2003 and is credited : with steady increases in seat belt use across the Nation. : In 2009, seat...

Preserve America Summit

Science.gov Websites

the recommendations, see the Executive Summary or the Full Report) More than a year has passed, and reports on each recommendation. Comprehensive Inventory (click to read report) Agencies involved: NPS , ACHP (with NCSHPO) Cultural Diversity (click to read report) Agencies involved: NPS Disasters (click to

Effects of Temperature on Sound Production and Auditory Abilities in the Striped Raphael Catfish Platydoras armatulus (Family Doradidae)

PubMed Central

Papes, Sandra; Ladich, Friedrich

2011-01-01

Background Sound production and hearing sensitivity of ectothermic animals are affected by the ambient temperature. This is the first study investigating the influence of temperature on both sound production and on hearing abilities in a fish species, namely the neotropical Striped Raphael catfish Platydoras armatulus. Methodology/Principal Findings Doradid catfishes produce stridulation sounds by rubbing the pectoral spines in the shoulder girdle and drumming sounds by an elastic spring mechanism which vibrates the swimbladder. Eight fish were acclimated for at least three weeks to 22°, then to 30° and again to 22°C. Sounds were recorded in distress situations when fish were hand-held. The stridulation sounds became shorter at the higher temperature, whereas pulse number, maximum pulse period and sound pressure level did not change with temperature. The dominant frequency increased when the temperature was raised to 30°C and the minimum pulse period became longer when the temperature decreased again. The fundamental frequency of drumming sounds increased at the higher temperature. Using the auditory evoked potential (AEP) recording technique, the hearing thresholds were tested at six different frequencies from 0.1 to 4 kHz. The temporal resolution was determined by analyzing the minimum resolvable click period (0.3–5 ms). The hearing sensitivity was higher at the higher temperature and differences were more pronounced at higher frequencies. In general, latencies of AEPs in response to single clicks became shorter at the higher temperature, whereas temporal resolution in response to double-clicks did not change. Conclusions/Significance These data indicate that sound characteristics as well as hearing abilities are affected by temperatures in fishes. Constraints imposed on hearing sensitivity at different temperatures cannot be compensated even by longer acclimation periods. These changes in sound production and detection suggest that acoustic orientation and communication are affected by temperature changes in the neotropical catfish P. armatulus. PMID:22022618

Doubled-lined eclipsing binary system KIC~2306740 with pulsating component discovered from Kepler space photometry

NASA Astrophysics Data System (ADS)

Yakut, Kadri

2015-08-01

We present a detailed study of KIC 2306740, an eccentric double-lined eclipsing binary system with a pulsating component.Archive Kepler satellite data were combined with newly obtained spectroscopic data with 4.2\\,m William Herschel Telescope(WHT). This allowed us to determine rather precise orbital and physical parameters of this long period, slightly eccentric, pulsating binary system. Duplicity effects are extracted from the light curve in order to estimate pulsation frequencies from the residuals.We modelled the detached binary system assuming non-conservative evolution models with the Cambridge STARS(TWIN) code.

Electronic device increases threshold sensitivity and removes noise from FM communications receiver

NASA Technical Reports Server (NTRS)

Conrad, W. M.; Loch, F. J.

1971-01-01

Threshold extension device connected between demodulator output and filter output minimizes clicking noise. Device consists of click-eliminating signal transfer channel with follow-and-hold circuit and detector for sensing click impulses. Final output consists of signal plus low level noise without high amplitude impulses.

Echolocation behaviour adapted to prey in foraging Blainville's beaked whale (Mesoplodon densirostris)

PubMed Central

Johnson, M; Hickmott, L.S; Aguilar Soto, N; Madsen, P.T

2007-01-01

Toothed whales echolocating in the wild generate clicks with low repetition rates to locate prey but then produce rapid sequences of clicks, called buzzes, when attempting to capture prey. However, little is known about the factors that determine clicking rates or how prey type and behaviour influence echolocation-based foraging. Here we study Blainville's beaked whales foraging in deep water using a multi-sensor DTAG that records both outgoing echolocation clicks and echoes returning from mesopelagic prey. We demonstrate that the clicking rate at the beginning of buzzes is related to the distance between whale and prey, supporting the presumption that whales focus on a specific prey target during the buzz. One whale showed a bimodal relationship between target range and clicking rate producing abnormally slow buzz clicks while attempting to capture large echoic targets, probably schooling prey, with echo duration indicating a school diameter of up to 4.3 m. These targets were only found when the whale performed tight circling manoeuvres spending up to five times longer in water volumes with large targets than with small targets. The result indicates that toothed whales in the wild can adjust their echolocation behaviour and movement for capture of different prey on the basis of structural echo information. PMID:17986434

Timing and context of dolphin clicks during and after mine simulator detection and marking in the open ocean

PubMed Central

Dibble, Dianna S.; Kennemer, Jaime A.

2018-01-01

ABSTRACT Two dolphins carrying cameras swam in the ocean as they searched for and marked mine simulators – buried, proud or moored. As the animals swam ahead of a boat they searched the ocean. Cameras on their harness recorded continuous sound and video. Once a target was detected, the dolphins received a marker to take to the simulator's location. During search and detection, dolphins made almost continuous trains of varying interval clicks. During the marking phase, shorter click trains were interrupted by periods of silence. As the dolphins marked simulators, they often produced victory squeals – pulse bursts that vary in duration, peak frequency and amplitude. Victory squeals were produced on 72% of marks. Sometimes after marking, or at other times during their long swims, the dolphins produced click packets. Packets typically consisted of two to 10 clicks with inter-click intervals of 7-117 ms followed by a silence of 223-983 ms. Click packets appeared unrelated with searching or marking. We suggest that the packets were used to improve signal to noise ratios for locating a boat or other distant object. Victory squeals produced when marking the targets suggest to us that the dolphins know when they have succeeded in this multipart task. PMID:29463515

Timing and context of dolphin clicks during and after mine simulator detection and marking in the open ocean.

PubMed

Ridgway, Sam H; Dibble, Dianna S; Kennemer, Jaime A

2018-02-20

Two dolphins carrying cameras swam in the ocean as they searched for and marked mine simulators - buried, proud or moored. As the animals swam ahead of a boat they searched the ocean. Cameras on their harness recorded continuous sound and video. Once a target was detected, the dolphins received a marker to take to the simulator's location. During search and detection, dolphins made almost continuous trains of varying interval clicks. During the marking phase, shorter click trains were interrupted by periods of silence. As the dolphins marked simulators, they often produced victory squeals - pulse bursts that vary in duration, peak frequency and amplitude. Victory squeals were produced on 72% of marks. Sometimes after marking, or at other times during their long swims, the dolphins produced click packets. Packets typically consisted of two to 10 clicks with inter-click intervals of 7-117 ms followed by a silence of 223-983 ms. Click packets appeared unrelated with searching or marking. We suggest that the packets were used to improve signal to noise ratios for locating a boat or other distant object. Victory squeals produced when marking the targets suggest to us that the dolphins know when they have succeeded in this multipart task. © 2018. Published by The Company of Biologists Ltd.

Dynamic allocation of attention to metrical and grouping accents in rhythmic sequences.

PubMed

Kung, Shu-Jen; Tzeng, Ovid J L; Hung, Daisy L; Wu, Denise H

2011-04-01

Most people find it easy to perform rhythmic movements in synchrony with music, which reflects their ability to perceive the temporal periodicity and to allocate attention in time accordingly. Musicians and non-musicians were tested in a click localization paradigm in order to investigate how grouping and metrical accents in metrical rhythms influence attention allocation, and to reveal the effect of musical expertise on such processing. We performed two experiments in which the participants were required to listen to isochronous metrical rhythms containing superimposed clicks and then to localize the click on graphical and ruler-like representations with and without grouping structure information, respectively. Both experiments revealed metrical and grouping influences on click localization. Musical expertise improved the precision of click localization, especially when the click coincided with a metrically strong beat. Critically, although all participants located the click accurately at the beginning of an intensity group, only musicians located it precisely when it coincided with a strong beat at the end of the group. Removal of the visual cue of grouping structures enhanced these effects in musicians and reduced them in non-musicians. These results indicate that musical expertise not only enhances attention to metrical accents but also heightens sensitivity to perceptual grouping.

Interrelationships between intranarial pressure and biosonar clicks in the bottlenose dolphin (Tursiops truncatus)

NASA Astrophysics Data System (ADS)

Elsberry, Wesley R.; Cranford, Ted W.; Ridgway, Sam H.; Carder, Donald A.; Vanbonn, William G.; Blackwood, Diane J.; Carr, Jennifer A.; Evans, William E.

2002-05-01

Three Atlantic bottlenose dolphins (Tursiops truncatus) were given a target recognition biosonar task. During their performance of the task, both acoustic data in the far field and pressure within the bony nasal passages were digitally recorded (Elsberry et al., 1999). Analysis of over 15000 biosonar clicks provided new insights into odontocete biosonar sound production and is consistent with acoustic and pressure data taken from white whales (Delphinapterus leucas) during biosonar (Ridgway and Carder, 1988). Our work provides the first evidence for a minimum intranarial pressure during biosonar click production for any odontocete (11.8+/-0.5 kPascals over basal pressure). All three subjects exhibited nearly the same minimum tranarial pressure difference during biosonar click production. Clicks produced at or near this minimum intranarial pressure exhibited a wide range of acoustic power values. The acoustic power of a biosonar click was not highly correlated with intranarial pressure (R2=0.116). The radiated acoustic energy in biosonar clicks ranged from 1 to 1370 microJoules. Estimates of mechanical work during pressurization events were produced using a piston/cylinder model and intranarial volume data from prepared specimens and computed tomography scans. Mechanical work during pressurization events ranged from 2.74 to 23.0 Joules, with an average of 10.3 Joules.

Cloud computing for genomic data analysis and collaboration.

PubMed

Langmead, Ben; Nellore, Abhinav

2018-04-01

Next-generation sequencing has made major strides in the past decade. Studies based on large sequencing data sets are growing in number, and public archives for raw sequencing data have been doubling in size every 18 months. Leveraging these data requires researchers to use large-scale computational resources. Cloud computing, a model whereby users rent computers and storage from large data centres, is a solution that is gaining traction in genomics research. Here, we describe how cloud computing is used in genomics for research and large-scale collaborations, and argue that its elasticity, reproducibility and privacy features make it ideally suited for the large-scale reanalysis of publicly available archived data, including privacy-protected data.

Targeted Acoustic Data Processing for Ocean Ecological Studies

NASA Astrophysics Data System (ADS)

Sidorovskaia, N.; Li, K.; Tiemann, C.; Ackleh, A. S.; Tang, T.; Ioup, G. E.; Ioup, J. W.

2015-12-01

The Gulf of Mexico is home to many species of deep diving marine mammals. In recent years several ecological studies have collected large volumes of Passive Acoustic Monitoring (PAM) data to investigate the effects of anthropogenic activities on protected and endangered marine mammal species. To utilize these data to their fullest potential for abundance estimates and habitat preference studies, automated detection and classification algorithms are needed to extract species acoustic encounters from a continuous stream of data. The species which phonate in overlapping frequency bands represent a particular challenge. This paper analyzes the performance of a newly developed automated detector for the classification of beaked whale clicks in the Northern Gulf of Mexico. Current used beaked whale classification algorithms rely heavily on experienced human operator involvement in manually associating potential events with a particular species of beaked whales. Our detection algorithm is two-stage: the detector is triggered when the species-representative phonation band energy exceeds the baseline detection threshold. Then multiple event attributes (temporal click duration, central frequency, frequency band, frequency sweep rate, Choi-Williams distribution shape indices) are measured. An attribute vector is then used to discriminate among different species of beaked whales present in the Gulf of Mexico and Risso's dolphins which were recognized to mask the detections of beaked whales in the case of widely used energy-band detectors. The detector is applied to the PAM data collected by the Littoral Acoustic Demonstration Center to estimate abundance trends of beaked whales in the vicinity of the 2010 oil spill before and after the disaster. This algorithm will allow automated processing with minimal operator involvement for new and archival PAM data. [The research is supported by a BP/GOMRI 2015-2017 consortium grant.

Male discrimination of receptive and unreceptive female calls by temporal features

PubMed Central

Elliott, Taffeta M.; Kelley, Darcy B.

2012-01-01

Summary In murky, crowded ponds in South Africa, female clawed frogs, Xenopus laevis (Daudin), vocalize to signal reproductive state. Female calls consist of acoustically similar clicks delivered in trains with characteristic rates. The rapping call of a sexually receptive female has a more rapid click rate [81 ms mean interclick interval (ICI)] than the ticking call of an unreceptive female (219 ms ICI). Rapping stimulates male advertisement calling, whereas ticking suppresses an already calling male. We examined how males label and discriminate female click rates. A labeling boundary experiment revealed that males perceive click rates between the means of rapping and ticking as lying on a continuum. They respond to 98 and 160 ms ICI as though to rapping and ticking, respectively. However, calling evoked by a click rate equally common to both calls (120 ms ICI) did not differ from the response to either rapping or ticking. A second experiment evaluated whether males discriminate click rates both labeled as ticking (180 and 219 ms ICI). Ticking suppresses advertising males, and suppressed males habituate (resume calling) to prolonged ticking. Both ticking stimuli suppressed males with equal effectiveness, and males habituated in equivalent amounts of time. When the stimulus was switched during habituation, no dishabituation occurred. We conclude that male labeling of click trains as rapping or ticking reflects an ambiguity resulting from the overlap in ICIs naturally occurring in the calls. Males do not respond differentially to click rates within the ticking category. Males thus combine discriminating and non-discriminating strategies in responding to the salient feature of female calls. PMID:17690231

Click Chemistry and Radiochemistry: The First 10 Years.

PubMed

Meyer, Jan-Philip; Adumeau, Pierre; Lewis, Jason S; Zeglis, Brian M

2016-12-21

The advent of click chemistry has had a profound influence on almost all branches of chemical science. This is particularly true of radiochemistry and the synthesis of agents for positron emission tomography (PET), single photon emission computed tomography (SPECT), and targeted radiotherapy. The selectivity, ease, rapidity, and modularity of click ligations make them nearly ideally suited for the construction of radiotracers, a process that often involves working with biomolecules in aqueous conditions with inexorably decaying radioisotopes. In the following pages, our goal is to provide a broad overview of the first 10 years of research at the intersection of click chemistry and radiochemistry. The discussion will focus on four areas that we believe underscore the critical advantages provided by click chemistry: (i) the use of prosthetic groups for radiolabeling reactions, (ii) the creation of coordination scaffolds for radiometals, (iii) the site-specific radiolabeling of proteins and peptides, and (iv) the development of strategies for in vivo pretargeting. Particular emphasis will be placed on the four most prevalent click reactions-the Cu-catalyzed azide-alkyne cycloaddition (CuAAC), the strain-promoted azide-alkyne cycloaddition (SPAAC), the inverse electron demand Diels-Alder reaction (IEDDA), and the Staudinger ligation-although less well-known click ligations will be discussed as well. Ultimately, it is our hope that this review will not only serve to educate readers but will also act as a springboard, inspiring synthetic chemists and radiochemists alike to harness click chemistry in even more innovative and ambitious ways as we embark upon the second decade of this fruitful collaboration.

77 FR 75602 - Safety Zone, Atlantic Intracoastal Waterway; Oak Island, NC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-21

... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket Number USCG-2012-1062] RIN...-2012-1062) in the ``SEARCH'' box and click ``SEARCH.'' Click on ``Submit a Comment'' on the line...://www.regulations.gov , type the docket number (USCG-2012-1062) in the ``SEARCH'' box and click ``SEARCH...

Iterated intracochlear reflection shapes the envelopes of basilar-membrane click responses

PubMed Central

Shera, Christopher A.

2015-01-01

Multiple internal reflection of cochlear traveling waves has been argued to provide a plausible explanation for the waxing and waning and other temporal structures often exhibited by the envelopes of basilar-membrane (BM) and auditory-nerve responses to acoustic clicks. However, a recent theoretical analysis of a BM click response measured in chinchilla concludes that the waveform cannot have arisen via any equal, repetitive process, such as iterated intracochlear reflection [Wit and Bell (2015), J. Acoust. Soc. Am. 138, 94–96]. Reanalysis of the waveform contradicts this conclusion. The measured BM click response is used to derive the frequency-domain transfer function characterizing every iteration of the loop. The selfsame transfer function that yields waxing and waning of the BM click response also captures the spectral features of ear-canal stimulus-frequency otoacoustic emissions measured in the same animal, consistent with the predictions of multiple internal reflection. Small shifts in transfer-function phase simulate results at different measurement locations and reproduce the heterogeneity of BM click response envelopes observed experimentally. PMID:26723327

'Megapclicks': acoustic click trains and buzzes produced during night-time foraging of humpback whales (Megaptera novaeangliae).

PubMed

Stimpert, Alison K; Wiley, David N; Au, Whitlow W L; Johnson, Mark P; Arsenault, Roland

2007-10-22

Humpback whales (Megaptera novaeangliae) exhibit a variety of foraging behaviours, but neither they nor any baleen whale are known to produce broadband clicks in association with feeding, as do many odontocetes. We recorded underwater behaviour of humpback whales in a northwest Atlantic feeding area using suction-cup attached, multi-sensor, acoustic tags (DTAGs). Here we describe the first recordings of click production associated with underwater lunges from baleen whales. Recordings of over 34000 'megapclicks' from two whales indicated relatively low received levels at the tag (between 143 and 154dB re 1 microPa pp), most energy below 2kHz, and interclick intervals often decreasing towards the end of click trains to form a buzz. All clicks were recorded during night-time hours. Sharp body rolls also occurred at the end of click bouts containing buzzes, suggesting feeding events. This acoustic behaviour seems to form part of a night-time feeding tactic for humpbacks and also expands the known acoustic repertoire of baleen whales in general.

The smallest quaternary ammonium salts with ether groups for high-performance electrochemical double layer capacitors† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc02755a Click here for additional data file.

PubMed Central

Han, Taihee; Park, Min-Sik; Kim, Jeonghun; Kim, Jung Ho

2016-01-01

Electrochemical double layer capacitors (EDLCs) are energy storage devices that have been used for a wide range of electronic applications. In particular, the electrolyte is one of the important components, directly related to the capacitance and stability. Herein, we first report a series of the smallest quaternary ammonium salts (QASs), with ether groups on tails and tetrafluoroborate (BF4) as an anion, for use in EDLCs. To find the optimal structure, various QASs with different sized head groups and ether-containing tail groups were systematically compared. Comparing two nearly identical structures with and without ether groups, QASs with oxygen atoms showed improved capacitance, proving that ions with oxygen atoms move more easily than their counterparts at lower electric fields. Moreover, the ether containing QASs showed low activation energy values of conductivities, leading to smaller IR drops during the charge and discharge processes, resulting in an overall higher capacitance. PMID:28959391

Evoked-potential recovery during double click stimulation in a beluga whale: implications for biosonar gain control.

PubMed

Supin, Alexander Ya; Popov, Vladimir V

2015-05-01

Auditory evoked potentials (AEPs) were recorded in a beluga whale Delphinapterus leucas using a double-pulse stimulation paradigm, specifically measuring the recovery (release from masking) of the second (test) response as a function of delay after the first (conditioning) pulse at various levels of the conditioning and test stimuli. The conditioning/test stimulus level ratio influenced the recovery time (the higher the ratio, the longer the recovery). This interrelation was used to evaluate the intensity/time trade in release from forward masking. Trade was evaluated as 32.2 dB per time decade. Data were considered as simulating interactions between the transmitted pulse and echo during echolocation, assuming that a transmitted sonar pulse produces forward masking of the echo response. With increased target distance, the attenuation of the echo may be compensated by the release from masking. According to the model, the compensation results in substantial stabilization of the echo response even if the intensity/time trade of release from masking is not precisely equal to the rate of echo attenuation with distance.

Click-MS: Tagless Protein Enrichment Using Bioorthogonal Chemistry for Quantitative Proteomics.

PubMed

Smits, Arne H; Borrmann, Annika; Roosjen, Mark; van Hest, Jan C M; Vermeulen, Michiel

2016-12-16

Epitope-tagging is an effective tool to facilitate protein enrichment from crude cell extracts. Traditionally, N- or C-terminal fused tags are employed, which, however, can perturb protein function. Unnatural amino acids (UAAs) harboring small reactive handles can be site-specifically incorporated into proteins, thus serving as a potential alternative for conventional protein tags. Here, we introduce Click-MS, which combines the power of site-specific UAA incorporation, bioorthogonal chemistry, and quantitative mass spectrometry-based proteomics to specifically enrich a single protein of interest from crude mammalian cell extracts. By genetic encoding of p-azido-l-phenylalanine, the protein of interest can be selectively captured using copper-free click chemistry. We use Click-MS to enrich proteins that function in different cellular compartments, and we identify protein-protein interactions, showing the great potential of Click-MS for interaction proteomics workflows.

Research, Collaboration, and Open Science Using Web 2.0

PubMed Central

Shee, Kevin; Strong, Michael; Guido, Nicholas J.; Lue, Robert A.; Church, George M.; Viel, Alain

2010-01-01

There is little doubt that the Internet has transformed the world in which we live. Information that was once archived in bricks and mortar libraries is now only a click away, and people across the globe have become connected in a manner inconceivable only 20 years ago. Although many scientists and educators have embraced the Internet as an invaluable tool for research, education and data sharing, some have been somewhat slower to take full advantage of emerging Web 2.0 technologies. Here we discuss the benefits and challenges of integrating Web 2.0 applications into undergraduate research and education programs, based on our experience utilizing these technologies in a summer undergraduate research program in synthetic biology at Harvard University. We discuss the use of applications including wiki-based documentation, digital brainstorming, and open data sharing via the Web, to facilitate the educational aspects and collaborative progress of undergraduate research projects. We hope to inspire others to integrate these technologies into their own coursework or research projects. PMID:23653712

Energy harvesting from mouse click of robot finger using piezoelectrics

NASA Astrophysics Data System (ADS)

Cha, Youngsu; Hong, Jin; Lee, Jaemin; Park, Jung-Min; Kim, Keehoon

2017-04-01

In this paper, we investigate the feasibility of energy harvesting from the mouse click motion using a piezoelectric energy transducer. Specifically, we use a robotic finger to realize repeatable mouse click motion. The robotic finger wears a glove with a pocket for including the piezoelectric material as an energy transducer. We propose a model for the energy harvesting system through the inverse kinematic framework of parallel joints in the finger and the electromechanical coupling equations of the piezoelectric material. Experiments are performed to elucidate the effect of the load resistance and the mouse click motion on energy harvesting.

Linking Metal Ions via Inorganic Click (iClick) Reactions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Veige, Adam

2015-11-17

This final report discusses the major objectives of the project, a discussion of the objectives achieved, a discussion of the objectives that failed, and finally, a discussion of future directions given the new knowledge obtained. This one-year seed project (with one year no-cost extension) contained three objectives: A) Expand the scope of iClick synthesis beyond AuI/AuI reactions. B) Elucidate a CuI-catalyzed iClick reaction. C) Synthesize and characterize tri- and tetra-metallic complexes as models for metallopolymers. Objectives A and C were achieved, whereas only parts of objective B were achieved.

Realtime Hourly Data Table

Science.gov Websites

Tables View the latest hourly text summary CLICK ON UNDERLINED HOUR / SHADED BOX FOR THE LATEST CYCLE 00z Dump Tables View the latest rap text summary CLICK ON UNDERLINED HOUR / SHADED BOX FOR THE LATEST CYCLE Data Dump Tables View the latest model data text summary NAM GFS GDS CLICK ON UNDERLINED HOUR / SHADED

Modeling User Behavior and Attention in Search

ERIC Educational Resources Information Center

Huang, Jeff

2013-01-01

In Web search, query and click log data are easy to collect but they fail to capture user behaviors that do not lead to clicks. As search engines reach the limits inherent in click data and are hungry for more data in a competitive environment, mining cursor movements, hovering, and scrolling becomes important. This dissertation investigates how…

PMC's Florida Bay & Adjacent Marine Systems Science Program

Science.gov Websites

Florida Bay and Adjacent Marine Systems Science Program Inverted image, click link below to view actual image and caption click to display actual image and caption Program Overview Management & - January 2002 >For more, click here to view the What's New Page... | Main | Overview | Management &

Comparison between ABR with click and narrow band chirp stimuli in children.

PubMed

Zirn, Stefan; Louza, Julia; Reiman, Viktor; Wittlinger, Natalie; Hempel, John-Martin; Schuster, Maria

2014-08-01

Click and chirp-evoked auditory brainstem responses (ABR) are applied for the estimation of hearing thresholds in children. The present study analyzes ABR thresholds across a large sample of children's ears obtained with both methods. The aim was to demonstrate the correlation between both methods using narrow band chirp and click stimuli. Click and chirp evoked ABRs were measured in 253 children aged from 0 to 18 years to determine their individual auditory threshold. The delay-compensated stimuli were narrow band CE chirps with either 2000 Hz or 4000 Hz center frequencies. Measurements were performed consecutively during natural sleep, and under sedation or general anesthesia. Threshold estimation was performed for each measurement by two experienced audiologists. Pearson-correlation analysis revealed highly significant correlations (r=0.94) between click and chirp derived thresholds for both 2 kHz and 4 kHz chirps. No considerable differences were observed either between different age ranges or gender. Comparing the thresholds estimated using ABR with click stimuli and chirp stimuli, only 0.8-2% for the 2000 Hz NB-chirp and 0.4-1.2% of the 4000 Hz NB-chirp measurements differed more than 15 dB for different degrees of hearing loss or normal hearing. The results suggest that either NB-chirp or click ABR is sufficient for threshold estimation. This holds for the chirp frequencies of 2000 Hz and 4000 Hz. The use of either click- or chirp-evoked ABR allows a reduction of recording time in young infants. Nevertheless, to cross-check the results of one of the methods, we recommend measurements with the other method as well. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Web Image Search Re-ranking with Click-based Similarity and Typicality.

PubMed

Yang, Xiaopeng; Mei, Tao; Zhang, Yong Dong; Liu, Jie; Satoh, Shin'ichi

2016-07-20

In image search re-ranking, besides the well known semantic gap, intent gap, which is the gap between the representation of users' query/demand and the real intent of the users, is becoming a major problem restricting the development of image retrieval. To reduce human effects, in this paper, we use image click-through data, which can be viewed as the "implicit feedback" from users, to help overcome the intention gap, and further improve the image search performance. Generally, the hypothesis visually similar images should be close in a ranking list and the strategy images with higher relevance should be ranked higher than others are widely accepted. To obtain satisfying search results, thus, image similarity and the level of relevance typicality are determinate factors correspondingly. However, when measuring image similarity and typicality, conventional re-ranking approaches only consider visual information and initial ranks of images, while overlooking the influence of click-through data. This paper presents a novel re-ranking approach, named spectral clustering re-ranking with click-based similarity and typicality (SCCST). First, to learn an appropriate similarity measurement, we propose click-based multi-feature similarity learning algorithm (CMSL), which conducts metric learning based on clickbased triplets selection, and integrates multiple features into a unified similarity space via multiple kernel learning. Then based on the learnt click-based image similarity measure, we conduct spectral clustering to group visually and semantically similar images into same clusters, and get the final re-rank list by calculating click-based clusters typicality and withinclusters click-based image typicality in descending order. Our experiments conducted on two real-world query-image datasets with diverse representative queries show that our proposed reranking approach can significantly improve initial search results, and outperform several existing re-ranking approaches.

Growing Applications of “Click Chemistry” for Bioconjugation in Contemporary Biomedical Research

PubMed Central

Nwe, Kido

2009-01-01

Summation This update summarizes the growing application of “click” chemistry in diverse areas such as bioconjugation, drug discovery, materials science, and radiochemistry. This update also discusses click chemistry reactions that proceed rapidly with high selectivity, specificity, and yield. Two important characteristics make click chemistry so attractive for assembling compounds, reagents, and biomolecules for preclinical and clinical applications. First, click reactions are bio-orthogonal; neither the reactants nor their product's functional groups interact with functionalized biomolecules. Second, the reactions proceed with ease under mild nontoxic conditions, such as at room temperature and, usually, in water. The copper-catalyzed Huisgen cycloaddition, azide-alkyne [3 + 2] dipolar cycloaddition, Staudinger ligation, and azide-phosphine ligation each possess these unique qualities. These reactions can be used to modify one cellular component while leaving others unharmed or untouched. Click chemistry has found increasing applications in all aspects of drug discovery in medicinal chemistry, such as for generating lead compounds through combinatorial methods. Bioconjugation via click chemistry is rigorously employed in proteomics and nucleic research. In radiochemistry, selective radiolabeling of biomolecules in cells and living organisms for imaging and therapy has been realized by this technology. Bifunctional chelating agents for several radionuclides useful for positron emission tomography and single-photon emission computed tomography imaging have also been prepared by using click chemistry. This review concludes that click chemistry is not the perfect conjugation and assembly technology for all applications, but provides a powerful, attractive alternative to conventional chemistry. This chemistry has proven itself to be superior in satisfying many criteria (e.g., biocompatibility, selectivity, yield, stereospecificity, and so forth); thus, one can expect it will consequently become a more routine strategy in the near future for a wide range of applications. PMID:19538051

Evoked potential recording during echolocation in a false killer whale Pseudorca crassidens (L)

NASA Astrophysics Data System (ADS)

Supin, Alexander Ya.; Nachtigall, Paul E.; Pawloski, Jeffrey; Au, Whitlow W. L.

2003-05-01

Auditory brainstem responses (ABRs) were recorded in a false killer whale while the animal echolocated a target. The ABR collection was triggered by echolocation clicks of the animal. In these conditions, the recorded ABR pattern contained a duplicate set of waves. A comparison of ABR wave delays recorded during echolocation with those recorded during regular external stimulation with experimenter generated clicks showed that the first set of waves may be a response to the emitted click whereas the second one may be a response to the echo. Both responses, to the emitted click and to the echo, were of comparable amplitude in spite of the intensity difference of these two sounds that may differ by more than 40 dB near the animal's head. This finding indicates the presence of some mechanism of releasing responses to echoes from masking by loud emitted clicks. The evoked-potential method may be productive to investigate these mechanisms.

Factors influencing clicking of banner ads on the WWW.

PubMed

Cho, Chang-Hoan

2003-04-01

This paper explores various factors influencing the clicking of banner ads on the WWW. The study indicates that several important variables affect the clicking of banner ads: (1) level of product involvement, (2) congruency between the content of a vehicle and the product category of a banner ad, (3) attitude toward the vehicle, and (4) overall attitude toward web advertising. For methodology, this study employed a between-group experimental design and measured real click-through rates with the aid of an online data collection technology called FileMaker Pro. Results of logistic regressions show that people are more likely to click banner ads if they are highly involved with products, perceive high synergy between web pages and ads, have a favorable attitude toward a vehicle, or have a favorable attitude toward web advertising in general. A total of 756 subjects participated in this research.

ECS DAAC Data Pools

NASA Astrophysics Data System (ADS)

Kiebuzinski, A. B.; Bories, C. M.; Kalluri, S.

2002-12-01

As part of its Earth Observing System (EOS), NASA supports operations for several satellites including Landsat 7, Terra, and Aqua. ECS (EOSDIS Core System) is a vast archival and distribution system and includes several Distributed Active Archive Centers (DAACs) located around the United States. EOSDIS reached a milestone in February when its data holdings exceeded one petabyte (1,000 terabytes) in size. It has been operational since 1999 and originally was intended to serve a large community of Earth Science researchers studying global climate change. The Synergy Program was initiated in 2000 with the purpose of exploring and expanding the use of remote sensing data beyond the traditional research community to the applications community including natural resource managers, disaster/emergency managers, urban planners and others. This included facilitating data access at the DAACs to enable non-researchers to exploit the data for their specific applications. The combined volume of data archived daily across the DAACs is of the order of three terabytes. These archived data are made available to the research community and to general users of ECS data. Currently, the average data volume distributed daily is two terabytes, which combined with an ever-increasing need for timely access to these data, taxes the ECS processing and archival resources for more real-time use than was previously intended for research purposes. As a result, the delivery of data sets to users was being delayed in many cases, to unacceptable limits. Raytheon, under the auspices of the Synergy Program, investigated methods at making data more accessible at a lower cost of resources (processing and archival) at the DAACs. Large on-line caches (as big as 70 Terabytes) of data were determined to be a solution that would allow users who require contemporary data to access them without having to pull it from the archive. These on-line caches are referred to as "Data Pools." In the Data Pool concept, data is inserted via subscriptions based on ECS events, for example, arrival of data matching a specific spatial context. Upon acquisition, these data are written to the Data Pools as well as to the permanent archive. The data is then accessed via a public Web interface, which provides a drilldown search, using data group, spatial, temporal and other flags. The result set is displayed as a list of ftp links to the data, which the user can click and directly download. Data Pool holdings are continuously renewed as the data is allowed to expire and is replaced by more current insertions. In addition, the Data Pool may also house data sets that though not contemporary, receive significant user attention, i.e. a Chernobyl-type of incident, a flood, or a forest fire. The benefits are that users who require contemporary data can access the data immediately (within 24 hours of acquisition) under a much improved access technique. Users not requiring contemporary data, benefit from the Data Pools by having greater archival and processing resources (and a shorter processing queue) made available to them. All users benefit now from the capability to have standing data orders for data matching a geographic context (spatial subscription), a capability also developed under the Synergy program. The Data Pools are currently being installed and checked at each of the DAACs. Additionally, several improvements to the search capabilities, data manipulation tools and overall storage capacity are being developed and will be installed in the First Quarter of 2003.

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View Submitted Projects | NOAA Gulf Spill Restoration

Science.gov Websites

that have been submitted to the trustees for consideration. To view details of an individual project , click the View icon on the list below or click the project marker on the map. To highlight the location of a project from the list, click the Show on Map icon. All projects that have met the posting

Sex and Ear Differences in Spontaneous and Click-Evoked Otoacoustic Emissions in Young Adults

ERIC Educational Resources Information Center

Snihur, Adrian W. K.; Hampson, Elizabeth

2011-01-01

Effects of sex and handedness on the production of spontaneous and click-evoked otoacoustic emissions (OAEs) were explored in a non-hearing impaired population (ages 17-25 years). A sex difference in OAEs, either produced spontaneously (spontaneous OAEs or SOAEs) or in response to auditory stimuli (click-evoked OAEs or CEOAEs) has been reported in…

Regenerative biomaterials that "click": simple, aqueous-based protocols for hydrogel synthesis, surface immobilization, and 3D patterning.

PubMed

Nimmo, Chelsea M; Shoichet, Molly S

2011-11-16

The click chemistry era has generated a library of versatile "spring-loaded" reactions that offer high yields, regio- and stereospecificity, and outstanding functional group tolerance. These powerful transformations are particularly advantageous for the design of sophisticated biomaterials that require high levels of precision and control, namely, materials that promote tissue regeneration such as hydrogels, 2D functionalized substrates, and 3D biomimetic scaffolds. In this review, the synthesis and application of regenerative biomaterials via click chemistry are summarized. Particular emphasis is placed on the copper(I)-catalyzed alkyne-azide cycloaddition, Diels-Alder cycloadditions, and thiol-click coupling.

Echolocation clicks from killer whales (Orcinus orca) feeding on herring (Clupea harengus).

PubMed

Simon, Malene; Wahlberg, Magnus; Miller, Lee A

2007-02-01

Echolocation clicks from Norwegian killer whales feeding on herring schools were recorded using a four-hydrophone array. The clicks had broadband bimodal frequency spectra with low and high frequency peaks at 24 and 108 kHz, respectively. The -10 dB bandwidth was 35 kHz. The average source level varied from 173 to 202 dB re 1 microPa (peak-to-peak) at 1 m. This is considerably lower than source levels described for Canadian killer whales foraging on salmon. It is suggested that biosonar clicks of Norwegian killer whales are adapted for localization of prey with high target strength and acute hearing abilities.

Specific labeling of zinc finger proteins using noncanonical amino acids and copper-free click chemistry.

PubMed

Kim, Younghoon; Kim, Sung Hoon; Ferracane, Dean; Katzenellenbogen, John A; Schroeder, Charles M

2012-09-19

Zinc finger proteins (ZFPs) play a key role in transcriptional regulation and serve as invaluable tools for gene modification and genetic engineering. Development of efficient strategies for labeling metalloproteins such as ZFPs is essential for understanding and controlling biological processes. In this work, we engineered ZFPs containing cysteine-histidine (Cys2-His2) motifs by metabolic incorporation of the unnatural amino acid azidohomoalanine (AHA), followed by specific protein labeling via click chemistry. We show that cyclooctyne promoted [3 + 2] dipolar cycloaddition with azides, known as copper-free click chemistry, provides rapid and specific labeling of ZFPs at high yields as determined by mass spectrometry analysis. We observe that the DNA-binding activity of ZFPs labeled by conventional copper-mediated click chemistry was completely abolished, whereas ZFPs labeled by copper-free click chemistry retain their sequence-specific DNA-binding activity under native conditions, as determined by electrophoretic mobility shift assays, protein microarrays, and kinetic binding assays based on Förster resonance energy transfer (FRET). Our work provides a general framework to label metalloproteins such as ZFPs by metabolic incorporation of unnatural amino acids followed by copper-free click chemistry.

Click- and chirp-evoked human compound action potentials

PubMed Central

Chertoff, Mark; Lichtenhan, Jeffery; Willis, Marie

2010-01-01

In the experiments reported here, the amplitude and the latency of human compound action potentials (CAPs) evoked from a chirp stimulus are compared to those evoked from a traditional click stimulus. The chirp stimulus was created with a frequency sweep to compensate for basilar membrane traveling wave delay using the O-Chirp equations from Fobel and Dau [(2004). J. Acoust. Soc. Am. 116, 2213–2222] derived from otoacoustic emission data. Human cochlear traveling wave delay estimates were obtained from derived compound band action potentials provided by Eggermont [(1979). J. Acoust. Soc. Am. 65, 463–470]. CAPs were recorded from an electrode placed on the tympanic membrane (TM), and the acoustic signals were monitored with a probe tube microphone attached to the TM electrode. Results showed that the amplitude and latency of chirp-evoked N1 of the CAP differed from click-evoked CAPs in several regards. For the chirp-evoked CAP, the N1 amplitude was significantly larger than the click-evoked N1s. The latency-intensity function was significantly shallower for chirp-evoked CAPs as compared to click-evoked CAPs. This suggests that auditory nerve fibers respond with more unison to a chirp stimulus than to a click stimulus. PMID:21117748

Specific Labeling of Zinc Finger Proteins using Non-canonical Amino Acids and Copper-free Click Chemistry

PubMed Central

Kim, Younghoon; Kim, Sung Hoon; Ferracane, Dean; Katzenellenbogen, John A.

2012-01-01

Zinc finger proteins (ZFPs) play a key role in transcriptional regulation and serve as invaluable tools for gene modification and genetic engineering. Development of efficient strategies for labeling metalloproteins such as ZFPs is essential for understanding and controlling biological processes. In this work, we engineered ZFPs containing cysteine-histidine (Cys2-His2) motifs by metabolic incorporation of the unnatural amino acid azidohomoalanine (AHA), followed by specific protein labeling via click chemistry. We show that cyclooctyne promoted [3 + 2] dipolar cycloaddition with azides, known as copper-free click chemistry, provides rapid and specific labeling of ZFPs at high yields as determined by mass spectrometry analysis. We observe that the DNA-binding activity of ZFPs labeled by conventional copper-mediated click chemistry was completely abolished, whereas ZFPs labeled by copper-free click chemistry retain their sequence-specific DNA-binding activity under native conditions, as determined by electrophoretic mobility shift assays, protein microarrays and kinetic binding assays based on Förster resonance energy transfer (FRET). Our work provides a general framework to label metalloproteins such as ZFPs by metabolic incorporation of unnatural amino acids followed by copper-free click chemistry. PMID:22871171

Echolocation click rates and behavior of foraging Hawaiian spinner dolphins

NASA Astrophysics Data System (ADS)

Benoit-Bird, Kelly J.; Au, Whitlow W. L.

2004-05-01

Groups of spinner dolphins work together to actively aggregate small animals in the deep-scattering layer that serve as their prey. Detailed information on dolphin foraging behavior, obtained with a 200-kHz multibeam sonar (Simrad MS2000), made it possible to correlate echolocation and foraging. Fifty-six groups of spinner dolphins foraging at night within a midwater micronekton sound-scattering layer were observed with the sonar. During sonar surveys, the rates of whistles and echolocation clicks were measured using four hydrophones at 6-m depth intervals. Significant differences in click rates were found between depths and between the different stages of foraging. Groups of foraging dolphins ranged in size from 16 to 28 dolphins. Click rates were not significantly affected by the number of dolphins in a foraging group. Contrary to initial predictions, click rates were relatively low when sonar data indicated that pairs of dolphins were actively feeding. Highest echolocation rates occurred within the scattering layer, during transitions between foraging states. Whistles were only detected when dolphins were not in a foraging formation and when animals were surfacing. This suggests clicks may be used directly or indirectly to cue group movement during foraging.

10. PHOTOGRAPHIC COPY OF ORIGINAL PHOTOGRAPH, DATED APRIL 29, 1926, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. PHOTOGRAPHIC COPY OF ORIGINAL PHOTOGRAPH, DATED APRIL 29, 1926, DOUBLE BARRACK, BUILDING 452, (ORIGINAL No. 655), SOUTH FRONT AND RIGHT SIDE, NATIONAL ARCHIVES PHOTOGRAPH, RECORD GROUP 92, GEOGRAPHIC FILE, 1922-1935, No. 600-1, FORT BLISS - Fort Bliss, 7th Cavalry Buildings, U.S. Army Air Defence Artillery Center & Fort Bliss, El Paso, El Paso County, TX

Postnatal development of echolocation abilities in a bottlenose dolphin (Tursiops truncatus): temporal organization.

PubMed

Favaro, Livio; Gnone, Guido; Pessani, Daniela

2013-03-01

In spite of all the information available on adult bottlenose dolphin (Tursiops truncatus) biosonar, the ontogeny of its echolocation abilities has been investigated very little. Earlier studies have reported that neonatal dolphins can produce both whistles and burst-pulsed sounds just after birth and that early-pulsed sounds are probably a precursor of echolocation click trains. The aim of this research is to investigate the development of echolocation signals in a captive calf, born in the facilities of the Acquario di Genova. A set of 81 impulsive sounds were collected from birth to the seventh postnatal week and six additional echolocation click trains were recorded when the dolphin was 1 year old. Moreover, behavioral observations, concurring with sound production, were carried out by means of a video camera. For each sound we measured five acoustic parameters: click train duration (CTD), number of clicks per train, minimum, maximum, and mean click repetition rate (CRR). CTD and number of clicks per train were found to increase with age. Maximum and mean CRR followed a decreasing trend with dolphin growth starting from the second postnatal week. The calf's first head scanning movement was recorded 21 days after birth. Our data suggest that in the bottlenose dolphin the early postnatal weeks are essential for the development of echolocation abilities and that the temporal features of the echolocation click trains remain relatively stable from the seventh postnatal week up to the first year of life. © 2013 Wiley Periodicals, Inc.

Neonate Auditory Brainstem Responses to CE-Chirp and CE-Chirp Octave Band Stimuli I: Versus Click and Tone Burst Stimuli.

PubMed

Cobb, Kensi M; Stuart, Andrew

The purpose of the study was to generate normative auditory brainstem response (ABR) wave component peak latency and amplitude values for neonates with air- and bone-conducted CE-Chirps and air-conducted CE-Chirp octave band stimuli (i.e., 500, 1000, 2000, and 4000 Hz). A second objective was to compare neonate ABRs to CE-Chirp stimuli with ABR responses to traditional click and tone burst stimuli with the same stimulus parameters. Participants were 168 healthy neonates. ABRs were obtained to air- and bone-conducted CE-Chirp and click stimuli and air-conducted CE-Chirp octave band and tone burst stimuli. The effects of stimulus level, rate, and polarity were examined with air-conducted CE-Chirps and clicks. The effect of stimulus level was also examined with bone-conducted CE-Chirps and clicks and air-conducted CE-Chirp octave band stimuli. In general, ABR wave V amplitudes to air- and bone-conducted CE-Chirp stimuli were significantly larger (p < 0.05) than those evoked to traditional click and tone burst stimuli. Systematic statistically significant (p < 0.05) wave V latency differences existed between the air- and bone-conducted CE-Chirp and CE-Chirp octave band stimuli relative to traditional click and tone burst stimuli. ABRs to air- and bone-conducted CE-Chirps and CE-Chirp octave band stimuli may be valuable in the assessment of newborn infants. However, the prognostic value of such stimuli needs to be validated.

Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention.

PubMed

Takei, Yoshiaki; Murata, Atsushi; Yamagishi, Kento; Arai, Satoshi; Nakamura, Hideki; Inoue, Takafumi; Takeoka, Shinji

2013-08-25

The powerful strategy of "intracellular click reaction" was used to retain a chemical Ca(2+) indicator in the cytosol. Specifically, a novel clickable Ca(2+) indicator "N3-fura-2 AM" was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca(2+) oscillation was observed for an extended period of time.

Just Click It: Undergraduate Procedures for the Copper(I)-Catalyzed Formation of 1,2,3-Triazoles from Azides and Terminal Acetylenes

ERIC Educational Resources Information Center

Sharpless, William D.; Peng Wu; Hansen, Trond Vidar; Lindberg, James G.

2005-01-01

The click chemistry uses only the most reliable reactions to build complex molecules from olefins, electrophiles and heteroatom linkers. A variation on Huisgen's azide-alkyne 1,2,3-triazole synthesis, the addition of the copper (I), the premium example of the click reaction, catalyst strongly activates terminal acetylenes towards the 1,3-dipole in…

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Magnetic Nanoparticle-Based Imaging of RNA Transcripts in Breast Cancer Cells

DTIC Science & Technology

2009-06-01

iron oxide NPs via thermal decomposition. - Prepared gold-coated iron oxide NPs. - Developed a click chemistry protocol (i.e. Cu-catalyzed terminal...D.L.J., Elias, D.R., Tsourkas, A. (2009) Comparative analysis of nanoparticle-antibody conjugations: carbodiimide versus click chemistry . Submitted...carbodiimide versus click chemistry . Submitted. APPENDICES: 1) Thorek, D.L.J., Tsourkas, A. (2008) Size, charge, and concentration dependent

Construct validity and inter-rater reliability of the Dutch activity measure for post-acute care "6-clicks" basic mobility form to assess the mobility of hospitalized patients.

PubMed

Geelen, Sven Jacobus Gertruda; Valkenet, Karin; Veenhof, Cindy

2018-05-12

To evaluate the construct validity and the inter-rater reliability of the Dutch Activity Measure for Post-Acute Care "6-clicks" Basic Mobility short form measuring the patient's mobility in Dutch hospital care. First, the "6-clicks" was translated by using a forward-backward translation protocol. Next, 64 patients were assessed by the physiotherapist to determine the validity while being admitted to the Internal Medicine wards of a university medical center. Six hypotheses were tested regarding the construct "mobility" which showed that: Better "6-clicks" scores were related to less restrictive pre-admission living situations (p = 0.011), less restrictive discharge locations (p = 0.001), more independence in activities of daily living (p = 0.001) and less physiotherapy visits (p < 0.001). A correlation was found between the "6-clicks" and length of stay (r= -0.408, p = 0.001), but not between the "6-clicks" and age (r= -0.180, p = 0.528). To determine the inter-rater reliability, an additional 50 patients were assessed by pairs of physiotherapists who independently scored the patients. Intraclass Correlation Coefficients of 0.920 (95%CI: 0.828-0.964) were found. The Kappa Coefficients for the individual items ranged from 0.649 (walking stairs) to 0.841 (sit-to-stand). The Dutch "6-clicks" shows a good construct validity and moderate-to-excellent inter-rater reliability when used to assess the mobility of hospitalized patients. Implications for Rehabilitation Even though various measurement tools have been developed, it appears the majority of physiotherapists working in a hospital currently do not use these tools as a standard part of their care. The Activity Measure for Post-Acute Care "6-clicks" Basic Mobility is the only tool which is designed to be short, easy to use within usual care and has been validated in the entire hospital population. This study shows that the Dutch version of the Activity Measure for Post-Acute Care "6-clicks" Basic Mobility form is a valid, easy to use, quick tool to assess the basic mobility of Dutch hospitalized patients.

Trapping self-propelled micromotors with microfabricated chevron and heart-shaped chips† †Electronic supplementary information (ESI) available: Supporting videos (S1; S2 and S3). See DOI: 10.1039/c3lc51419f Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file.

PubMed Central

Restrepo-Pérez, Laura; Soler, Lluís; Martínez-Cisneros, Cynthia S.; Schmidt, Oliver G.

2014-01-01

We demonstrate that catalytic micromotors can be trapped in microfluidic chips containing chevron and heart-shaped structures. Despite the challenge presented by the reduced size of the traps, microfluidic chips with different trapping geometries can be fabricated via replica moulding. We prove that these microfluidic chips can capture micromotors without the need for any external mechanism to control their motion. PMID:24643940

Advanced unambiguous state discrimination attack and countermeasure strategy in a practical B92 QKD system

NASA Astrophysics Data System (ADS)

Ko, Heasin; Choi, Byung-Seok; Choe, Joong-Seon; Youn, Chun Ju

2018-01-01

Even though unconditional security of B92 quantum key distribution (QKD) system is based on the assumption of perfect positive-operator-valued measures, practical B92 systems only utilize two projective measurements. Unfortunately, such implementation may degrade the security of the B92 QKD system due to Eve's potential attack exploiting the imperfection of system. In this paper, we propose an advanced attack strategy with an unambiguous state discrimination (USD) measurement which makes practical B92 QKD systems insecure even under a lossless channel. In addition, we propose an effective countermeasure against the advanced USD attack model by monitoring double-click events. We further address a fundamental approach to make the B92 QKD system tolerable to attack strategies with USD measurements using a multi-qubit scheme.

Neurons sensitive to narrow ranges of repetitive acoustic transients in the medial geniculate body of the cat.

PubMed

Rouiller, E; de Ribaupierre, F

1982-01-01

Neuronal activity was recorded in the medial geniculate body (MGB) of nitrous oxide anaesthetized, paralysed cats in response to click trains. For most cells responding to these stimuli the spike discharges are precisely time locked to individual clicks within the train. The present study has revealed that, apart from the normal "locker" response being characterized by a monotonic decrease in the entrainment as the frequency of the clicks within the train increases, there is a small population of "lockers" which show a non-monotonic response to increasing click frequency. 41% of these non-monotonic cells were not at all entrained by the lowest click rates and had time-locked responses for very restricted frequency ranges. These particular non-monotonic "lockers" were more commonly-found in the posterior part of the pars lateralis and in the suprageniculate nucleus. These cells might be involved in the temporal coding of natural sounds such as animal vocalizations and the cat's purr.

Are There Differences Depending on the Device Used to Complete a Web Survey (PC or Smartphone) for Order-by-Click Questions?

ERIC Educational Resources Information Center

Revilla, Melanie

2017-01-01

The development of web surveys has been accompanied by the emergence of new scales, taking advantages of the visual and interactive features provided by the Internet like drop-down menus, sliders, drag-and-drop, or order-by-click scales. This article focuses on the order-by-click scales, studying the comparability of the data obtained for this…

Possible age-related hearing loss (presbycusis) and corresponding change in echolocation parameters in a stranded Indo-Pacific humpback dolphin.

PubMed

Li, Songhai; Wang, Ding; Wang, Kexiong; Hoffmann-Kuhnt, Matthias; Fernando, Nimal; Taylor, Elizabeth A; Lin, Wenzhi; Chen, Jialin; Ng, Timothy

2013-11-15

The hearing and echolocation clicks of a stranded Indo-Pacific humpback dolphin (Sousa chinensis) in Zhuhai, China, were studied. This animal had been repeatedly observed in the wild before it was stranded and its age was estimated to be ~40 years. The animal's hearing was measured using a non-invasive auditory evoked potential (AEP) method. Echolocation clicks produced by the dolphin were recorded when the animal was freely swimming in a 7.5 m (width)×22 m (length)×4.8 m (structural depth) pool with a water depth of ~2.5 m. The hearing and echolocation clicks of the studied dolphin were compared with those of a conspecific younger individual, ~13 years of age. The results suggested that the cut-off frequency of the high-frequency hearing of the studied dolphin was ~30-40 kHz lower than that of the younger individual. The peak and centre frequencies of the clicks produced by the older dolphin were ~16 kHz lower than those of the clicks produced by the younger animal. Considering that the older dolphin was ~40 years old, its lower high-frequency hearing range with lower click peak and centre frequencies could probably be explained by age-related hearing loss (presbycusis).

The Source Parameters of Echolocation Clicks from Captive and Free-Ranging Yangtze Finless Porpoises (Neophocaena asiaeorientalis asiaeorientalis).

PubMed

Fang, Liang; Wang, Ding; Li, Yongtao; Cheng, Zhaolong; Pine, Matthew K; Wang, Kexiong; Li, Songhai

2015-01-01

The clicks of Yangtze finless porpoises (Neophocaena asiaeorientalis asiaeorientalis) from 7 individuals in the tank of Baiji aquarium, 2 individuals in a netted pen at Shishou Tian-e-zhou Reserve and 4 free-ranging individuals at Tianxingzhou were recorded using a broadband digital recording system with four element hydrophones. The peak-to-peak apparent source level (ASL_pp) of clicks from individuals at the Baiji aquarium was 167 dB re 1 μPa with mean center frequency of 133 kHz, -3dB bandwidth of 18 kHz and -10 dB duration of 58 μs. The ASL_pp of clicks from individuals at the Shishou Tian-e-zhou Reserve was 180 dB re 1 μPa with mean center frequency of 128 kHz, -3dB bandwidth of 20 kHz and -10 dB duration of 39 μs. The ASL_pp of clicks from individuals at Tianxingzhou was 176 dB re 1 μPa with mean center frequency of 129 kHz, -3dB bandwidth of 15 kHz and -10 dB duration of 48 μs. Differences between the source parameters of clicks among the three groups of finless porpoises suggest these animals adapt to their echolocation signals depending on their surroundings.

Discriminating features of echolocation clicks of melon-headed whales (Peponocephala electra), bottlenose dolphins (Tursiops truncatus), and Gray's spinner dolphins (Stenella longirostris longirostris).

PubMed

Baumann-Pickering, Simone; Wiggins, Sean M; Hildebrand, John A; Roch, Marie A; Schnitzler, Hans-Ulrich

2010-10-01

Spectral parameters were used to discriminate between echolocation clicks produced by three dolphin species at Palmyra Atoll: melon-headed whales (Peponocephala electra), bottlenose dolphins (Tursiops truncatus) and Gray's spinner dolphins (Stenella longirostris longirostris). Single species acoustic behavior during daytime observations was recorded with a towed hydrophone array sampling at 192 and 480 kHz. Additionally, an autonomous, bottom moored High-frequency Acoustic Recording Package (HARP) collected acoustic data with a sampling rate of 200 kHz. Melon-headed whale echolocation clicks had the lowest peak and center frequencies, spinner dolphins had the highest frequencies and bottlenose dolphins were nested in between these two species. Frequency differences were significant. Temporal parameters were not well suited for classification. Feature differences were enhanced by reducing variability within a set of single clicks by calculating mean spectra for groups of clicks. Median peak frequencies of averaged clicks (group size 50) of melon-headed whales ranged between 24.4 and 29.7 kHz, of bottlenose dolphins between 26.7 and 36.7 kHz, and of spinner dolphins between 33.8 and 36.0 kHz. Discriminant function analysis showed the ability to correctly discriminate between 93% of melon-headed whales, 75% of spinner dolphins and 54% of bottlenose dolphins.

The effects of preceding lead-alone and lag-alone click trains on the buildup of echo suppression.

PubMed

Bishop, Christopher W; Yadav, Deepak; London, Sam; Miller, Lee M

2014-08-01

Spatial perception in echoic environments is influenced by recent acoustic history. For instance, echo suppression becomes more effective or "builds up" with repeated exposure to echoes having a consistent acoustic relationship to a temporally leading sound. Four experiments were conducted to investigate how buildup is affected by prior exposure to unpaired lead-alone or lag-alone click trains. Unpaired trains preceded lead-lag click trains designed to evoke and assay buildup. Listeners reported how many sounds they heard from the echo hemifield during the lead-lag trains. Stimuli were presented in free field (experiments 1 and 4) or dichotically through earphones (experiments 2 and 3). In experiment 1, listeners reported more echoes following a lead-alone train compared to a period of silence. In contrast, listeners reported fewer echoes following a lag-alone train; similar results were observed with earphones. Interestingly, the effects of lag-alone click trains on buildup were qualitatively different when compared to a no-conditioner trial type in experiment 4. Finally, experiment 3 demonstrated that the effects of preceding click trains on buildup cannot be explained by a change in counting strategy or perceived click salience. Together, these findings demonstrate that echo suppression is affected by prior exposure to unpaired stimuli.

Luciferase from Fulgeochlizus bruchi (Coleoptera:Elateridae), a Brazilian click-beetle with a single abdominal lantern: molecular evolution, biological function and comparison with other click-beetle luciferases.

PubMed

Amaral, Danilo T; Prado, Rogilene A; Viviani, Vadim R

2012-07-01

Bioluminescent click-beetles emit a wide range of bioluminescence colors (λ(Max) = 534-594 nm) from thoracic and abdominal lanterns, which are used for courtship. Only the luciferases from Pyrophorus and Pyrearinus species were cloned and sequenced. The Brazilian Fulgeochlizus bruchi click-beetle, which inhabits the Central-west Cerrado (Savannas), is noteworthy because, differently from other click-beetles, the adult stage displays only a functional abdominal lantern, which produces a bright green bioluminescence for sexual attraction purposes, and lacks functional thoracic lanterns. We cloned the cDNA for the abdominal lantern luciferase of this species. Notably, the primary sequence of this luciferase showed slightly higher identity with the green emitting dorsal lantern luciferases of the Pyrophorus genus instead of the abdominal lanterns luciferases. This luciferase displays a blue-shifted spectrum (λ(Max) = 540 nm), which is pH-insensitive from pH 7.5 to 9.5 and undergoes a slight red shift and broadening above this pH; the lowest K(M) for luciferin among studied click-beetle luciferases, and the highest optimum pH (9.0) ever reported for a beetle luciferase. At pH 9.0, the K(M) for luciferin increases, showing a decrease of affinity for this substrate, despite the higher activity. The slow luminescence decay rate of F. bruchi luciferase in vitro reaction could be an adaptation of this luciferase for the long and sustained in vivo luminescence display of the click-beetle during the courtship, and could be useful for in vivo intracellular imaging.

Auditory Brainstem Responses for Click and CE-chirp Stimuli in Individuals with and without Occupational Noise Exposure

PubMed Central

Pushpalatha, Zeena Venkatacheluvaiah; Konadath, Sreeraj

2016-01-01

Introduction: Encoding of CE-chirp and click stimuli in auditory system was studied using auditory brainstem responses (ABRs) among individuals with and without noise exposure. Materials and Methods: The study consisted of two groups. Group 1 (experimental group) consisted of 20 (40 ears) individuals exposed to occupational noise with hearing thresholds within 25 dB HL. They were further divided into three subgroups based on duration of noise exposure (0–5 years of exposure-T1, 5–10 years of exposure-T2, and >10 years of exposure-T3). Group 2 (control group) consisted of 20 individuals (40 ears). Absolute latency and amplitude of waves I, III, and V were compared between the two groups for both click and CE-chirp stimuli. T1, T2, and T3 groups were compared for the same parameters to see the effect of noise exposure duration on CE-chirp and click ABR. Result: In Click ABR, while both the parameters for wave III were significantly poorer for the experimental group, wave V showed a significant decline in terms of amplitude only. There was no significant difference obtained for any of the parameters for wave I. In CE-Chirp ABR, the latencies for all three waves were significantly prolonged in the experimental group. However, there was a significant decrease in terms of amplitude in only wave V for the same group. Discussion: Compared to click evoked ABR, CE-Chirp ABR was found to be more sensitive in comparison of latency parameters in individuals with occupational noise exposure. Monitoring of early pathological changes at the brainstem level can be studied effectively by using CE-Chirp stimulus in comparison to click stimulus. Conclusion: This study indicates that ABR's obtained with CE-chirp stimuli serves as an effective tool to identify the early pathological changes due to occupational noise exposure when compared to click evoked ABR. PMID:27762255

Binaural interaction in the auditory brainstem response: a normative study.

PubMed

Van Yper, Lindsey N; Vermeire, Katrien; De Vel, Eddy F J; Battmer, Rolf-Dieter; Dhooge, Ingeborg J M

2015-04-01

Binaural interaction can be investigated using auditory evoked potentials. A binaural interaction component can be derived from the auditory brainstem response (ABR-BIC) and is considered evidence for binaural interaction at the level of the brainstem. Although click ABR-BIC has been investigated thoroughly, data on 500 Hz tone-burst (TB) ABR-BICs are scarce. In this study, characteristics of click and 500 Hz TB ABR-BICs are described. Furthermore, reliability of both click and 500 Hz TB ABR-BIC are investigated. Eighteen normal hearing young adults (eight women, ten men) were included. ABRs were recorded in response to clicks and 500 Hz TBs. ABR-BICs were derived by subtracting the binaural response from the sum of the monaural responses measured in opposite ears. Good inter-rater reliability is obtained for both click and 500 Hz TB ABR-BICs. The most reliable peak in click ABR-BIC occurs at a mean latency of 6.06 ms (SD 0.354 ms). Reliable 500 Hz TB ABR-BIC are obtained with a mean latency of 9.47 ms (SD 0.678 ms). Amplitudes are larger for 500 Hz TB ABR-BIC than for clicks. The most reliable peak in click ABR-BIC occurs at the downslope of wave V. Five hundred Hertz TB ABR-BIC is characterized by a broad positivity occurring at the level of wave V. The ABR-BIC is a useful technique to investigate binaural interaction in certain populations. Examples are bilateral hearing aid users, bilateral cochlear implant users and bimodal listeners. The latter refers to the combination of unilateral cochlear implantation and contralateral residual hearing. The majority of these patients have residual hearing in the low frequencies. The current study suggests that 500 Hz TB ABR-BIC may be a suitable technique to assess binaural interaction in this specific population of cochlear implant users. Copyright © 2014 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.

Clicks versus Citations: Click Count as a Metric in High Energy Physics Publishing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bitton, Ayelet; /UC, San Diego /SLAC

2011-06-22

High-energy physicists worldwide rely on online resources such as SPIRES and arXiv to perform gather research and share their own publications. SPIRES is a tool designed to search the literature within high-energy physics, while arXiv provides the actual full-text documents of this literature. In high-energy physics, papers are often ranked according to the number of citations they acquire - meaning the number of times a later paper references the original. This paper investigates the correlation between the number of times a paper is clicked in order to be downloaded and the number of citations it receives following the click. Itmore » explores how physicists truly read what they cite.« less

One Click to the Cosmos: The AstroPix Image Archive

NASA Astrophysics Data System (ADS)

Hurt, Robert L.; Llamas, J.; Squires, G. K.; Brinkworth, C.; X-ray Center, Chandra; ESO/ESA; Science Center, Spitzer; STScI

2013-01-01

Imagine a single website that acts as a portal to the entire wealth of public imagery spanning the world's observatories. This is the goal of the AstroPix project (astropix.ipac.caltech.edu), and you can use it today! Although still in a beta development state, this past year has seen the inclusion of thousands of images spanning some of the most prominent observatories in the world, including Chandra, ESO, Galex, Herschel, Hubble, Spitzer, and WISE, with more on the way. The archive is unique as it is built around the Astronomical Visualization Metadata (AVM) standard, which captures the rich contextual information for each image. This ranges from titles and descriptions, to color representations and observation details, to sky coordinates. AVM enables AstroPix imagery to be used in a variety of unique ways that benefit formal and informal education as well as astronomers and the general public. Visitors to Astropix can search the database using simple free-text queries, or use a structured search (similar to "Smart Playlists" found in iTunes, for example). We are also developing public application programming interfaces (APIs) to allow third party software and websites to access the growing content for a variety of uses (planetarium software, museum kiosks, mobile apps, and creative web interfaces, to name a few). Contributing image assets to AstroPix is as easy as tagging the images with the relevant metadata and including the web links to the images in a simple RSS feed. We will cover some of the latest information about tools to contribute images to AstroPix and ways to use the site.

From "sapienza" to "sapienza, State Archives in Rome". a Looping Effect Bringing Back to the Original Source Comunication and Culture by Innovative and Low Cost 3d Surveying, Imaging Systems and GIS Applications

NASA Astrophysics Data System (ADS)

Paolini, P.; Forti, G.; Catalani, G.; Lucchetti, S.; Menghini, A.; Mirandola, A.; Pistacchio, S.; Porzia, U.; Roberti, M.

2016-04-01

High Quality survey models, realized by multiple Low Cost methods and technologies, as a container to sharing Cultural and Archival Heritage, this is the aim guiding our research, here described in its primary applications. The SAPIENZA building, a XVI century masterpiece that represented the first unified headquarters of University in Rome, plays since year 1936, when the University moved to its newly edified campus, the role of the main venue for the State Archives. By the collaboration of a group of students of the Architecture Faculty, some integrated survey methods were applied on the monument with success. The beginning was the topographic survey, creating a reference on ground and along the monument for the upcoming applications, a GNNS RTK survey followed georeferencing points on the internal courtyard. Dense stereo matching photogrammetry is nowadays an accepted method for generating 3D survey models, accurate and scalable; it often substitutes 3D laser scanning for its low cost, so that it became our choice. Some 360° shots were planned for creating panoramic views of the double portico from the courtyard, plus additional single shots of some lateral spans and of pillars facing the court, as a single operation with a double finality: to create linked panotours with hotspots to web-linked databases, and 3D textured and georeferenced surface models, allowing to study the harmonic proportions of the classical architectural order. The use of free web Gis platforms, to load the work in Google Earth and the realization of low cost 3D prototypes of some representative parts, has been even performed.

'It just has to click': Internists' views of: what constitutes productive interactions with chronically ill patients.

PubMed

Kromme, N M H; Ahaus, C T B; Gans, R O B; van de Wiel, H B M

2016-05-27

According to the Chronic Care Model, productive interactions are crucial to patient outcomes. Despite productive interactions being at the heart of the Model, however, it is unclear what constitutes such an interaction. The aim of this study was to gain a better understanding of physician views of productive interactions with the chronically ill. We conducted a qualitative study and interviewed 20 internists working in an academic hospital. The data were analyzed using a constructivist approach of grounded theory. To categorize the data, a coding process within which a code list was developed and tested with two other coders was conducted. The participants engaged in goal-directed reasoning when reflecting on productive interactions. This resulted in the identification of four goal orientations: (a) health outcome; (b) satisfaction; (c) medical process; and (d) collaboration. Collaboration appeared to be conditional for reaching medical process goals and ultimately health outcome and satisfaction goals. Achieving rapport with the patient ('clicking,' in the term of the participants) was found to be a key condition that catalyzed collaboration goals. Clicking appeared to be seen as a somewhat unpredictable phenomenon that might or might not emerge, which one had to accept and work with. Goal orientations were found to be related to the specific medical context (i.e., a participant's subspecialty and the nature of a patient's complaint). The participants viewed a productive interaction as essentially goal-directed, catalyzed by the two parties clicking, and dependent on the nature of a patient's complaint. Using the findings, we developed a conceptual process model with the four goal orientations as wheels and with clicking in the center as a flywheel. Because clicking was viewed as important, but somewhat unpredictable, teaching physicians how to click, while taking account of the medical context, may warrant greater attention.

Contralateral Inhibition of Click- and Chirp-Evoked Human Compound Action Potentials

PubMed Central

Smith, Spencer B.; Lichtenhan, Jeffery T.; Cone, Barbara K.

2017-01-01

Cochlear outer hair cells (OHC) receive direct efferent feedback from the caudal auditory brainstem via the medial olivocochlear (MOC) bundle. This circuit provides the neural substrate for the MOC reflex, which inhibits cochlear amplifier gain and is believed to play a role in listening in noise and protection from acoustic overexposure. The human MOC reflex has been studied extensively using otoacoustic emissions (OAE) paradigms; however, these measurements are insensitive to subsequent “downstream” efferent effects on the neural ensembles that mediate hearing. In this experiment, click- and chirp-evoked auditory nerve compound action potential (CAP) amplitudes were measured electrocochleographically from the human eardrum without and with MOC reflex activation elicited by contralateral broadband noise. We hypothesized that the chirp would be a more optimal stimulus for measuring neural MOC effects because it synchronizes excitation along the entire length of the basilar membrane and thus evokes a more robust CAP than a click at low to moderate stimulus levels. Chirps produced larger CAPs than clicks at all stimulus intensities (50–80 dB ppeSPL). MOC reflex inhibition of CAPs was larger for chirps than clicks at low stimulus levels when quantified both in terms of amplitude reduction and effective attenuation. Effective attenuation was larger for chirp- and click-evoked CAPs than for click-evoked OAEs measured from the same subjects. Our results suggest that the chirp is an optimal stimulus for evoking CAPs at low stimulus intensities and for assessing MOC reflex effects on the auditory nerve. Further, our work supports previous findings that MOC reflex effects at the level of the auditory nerve are underestimated by measures of OAE inhibition. PMID:28420960

Isosteric Substitution of 4H-1,2,4-Triazole by 1H-1,2,3-Triazole in Isophthalic Derivative Enabled Hydrogel Formation for Controlled Drug Delivery.

PubMed

Häring, Marleen; Rodríguez-López, Julio; Grijalvo, Santiago; Tautz, Markus; Eritja, Ramón; Martín, Víctor S; Díaz Díaz, David

2018-02-20

In this work, we demonstrated that the simple substitution of the 1,2,4-triazole moiety in 5-(4H-1,2,4-triazol-4-yl)isophthalic acid (5-TIA) by the 1H-1,2,3-triazol-5-yl unit enables the preparation of a hydrogelator (click-TIA). In sharp contrast to 5-TIA, its isostere click-TIA undergoes self-assembly in water upon sonication, leading to the formation of stable supramolecular viscoelastic hydrogels with a critical gelation concentration of 6 g/L. Hydrogels made of click-TIA as well as hybrid hydrogels made of the mixture click-TIA + 5-TIA (molar ratio 1:0.2) were used to compare different properties of the materials (i.e., rheological properties, thermal properties, mechanical stability, morphology). In terms of toxicity, neither click-TIA nor 5-TIA showed cytotoxic effects on cellular viability of HeLa cells up to 2.3 × 10 -3 g/L when compared to untreated cells incubated with DMSO. Furthermore, the hydrogels were used for the encapsulation and in vitro controlled release of oxytetracycline that followed first-order kinetics. For the hydrogel made of click-TIA, a maximum drug release of ∼60% was reached after ∼8 h within a pH range between 6.5 and 10. However, the release rate was reduced to approximately half of its value at pH values between 1.2 and 5.0, whereas the use of hybrid hydrogels made of click-TIA + 5-TIA allowed to reduce the original rate at pH ≤ 6.5.

The auditory P50 component to onset and offset of sound

PubMed Central

Pratt, Hillel; Starr, Arnold; Michalewski, Henry J.; Bleich, Naomi; Mittelman, Nomi

2008-01-01

Objective: The auditory Event-Related Potentials (ERP) component P50 to sound onset and offset have been reported to be similar, but their magnetic homologue has been reported absent to sound offset. We compared the spatio-temporal distribution of cortical activity during P50 to sound onset and offset, without confounds of spectral change. Methods: ERPs were recorded in response to onsets and offsets of silent intervals of 0.5 s (gaps) appearing randomly in otherwise continuous white noise and compared to ERPs to randomly distributed click pairs with half second separation presented in silence. Subjects were awake and distracted from the stimuli by reading a complicated text. Measures of P50 included peak latency and amplitude, as well as source current density estimates to the clicks and sound onsets and offsets. Results P50 occurred in response to noise onsets and to clicks, while to noise offset it was absent. Latency of P50 was similar to noise onset (56 msec) and to clicks (53 msec). Sources of P50 to noise onsets and clicks included bilateral superior parietal areas. In contrast, noise offsets activated left inferior temporal and occipital areas at the time of P50. Source current density was significantly higher to noise onset than offset in the vicinity of the temporo-parietal junction. Conclusions: P50 to sound offset is absent compared to the distinct P50 to sound onset and to clicks, at different intracranial sources. P50 to stimulus onset and to clicks appears to reflect preattentive arousal by a new sound in the scene. Sound offset does not involve a new sound and hence the absent P50. Significance: Stimulus onset activates distinct early cortical processes that are absent to offset. PMID:18055255

Fast degradable citrate-based bone scaffold promotes spinal fusion.

PubMed

Tang, Jiajun; Guo, Jinshan; Li, Zhen; Yang, Cheng; Xie, Denghui; Chen, Jian; Li, Shengfa; Li, Shaolin; Kim, Gloria B; Bai, Xiaochun; Zhang, Zhongmin; Yang, Jian

2015-07-21

It is well known that high rates of fusion failure and pseudoarthrosis development (5~35%) are concomitant in spinal fusion surgery, which was ascribed to the shortage of suitable materials for bone regeneration. Citrate was recently recognized to play an indispensable role in enhancing osteconductivity and osteoinductivity, and promoting bone formation. To address the material challenges in spinal fusion surgery, we have synthesized mechanically robust and fast degrading citrate-based polymers by incorporating N-methyldiethanolamine (MDEA) into clickable poly(1, 8-octanediol citrates) (POC-click), referred to as POC-M-click. The obtained POC-M-click were fabricated into POC-M-click-HA matchstick scaffolds by compositing with hydroxyapatite (HA) for interbody spinal fusion in a rabbit model. Spinal fusion was analyzed by radiography, manual palpation, biomechanical testing, and histological evaluation. At 4 and 8 weeks post surgery, POC-M-click-HA scaffolds presented optimal degradation rates that facilitated faster new bone formation and higher spinal fusion rates (11.2±3.7, 80±4.5 at week 4 and 8, respectively) than the poly(L-lactic acid)-HA (PLLA-HA) control group (9.3±2.4 and 71.1±4.4) (p<0.05). The POC-M-click-HA scaffold-fused vertebrates possessed a maximum load and stiffness of 880.8±14.5 N and 843.2±22.4 N/mm, respectively, which were also much higher than those of the PLLA-HA group (maximum: 712.0±37.5 N, stiffness: 622.5±28.4 N/mm, p<0.05). Overall, the results suggest that POC-M-click-HA scaffolds could potentially serve as promising bone grafts for spinal fusion applications.

Fast degradable citrate-based bone scaffold promotes spinal fusion

PubMed Central

Tang, Jiajun; Guo, Jinshan; Li, Zhen; Yang, Cheng; Xie, Denghui; Chen, Jian; Li, Shengfa; Li, Shaolin; Kim, Gloria B.; Bai, Xiaochun; Zhang, Zhongmin; Yang, Jian

2015-01-01

It is well known that high rates of fusion failure and pseudoarthrosis development (5~35%) are concomitant in spinal fusion surgery, which was ascribed to the shortage of suitable materials for bone regeneration. Citrate was recently recognized to play an indispensable role in enhancing osteconductivity and osteoinductivity, and promoting bone formation. To address the material challenges in spinal fusion surgery, we have synthesized mechanically robust and fast degrading citrate-based polymers by incorporating N-methyldiethanolamine (MDEA) into clickable poly(1, 8-octanediol citrates) (POC-click), referred to as POC-M-click. The obtained POC-M-click were fabricated into POC-M-click-HA matchstick scaffolds by compositing with hydroxyapatite (HA) for interbody spinal fusion in a rabbit model. Spinal fusion was analyzed by radiography, manual palpation, biomechanical testing, and histological evaluation. At 4 and 8 weeks post surgery, POC-M-click-HA scaffolds presented optimal degradation rates that facilitated faster new bone formation and higher spinal fusion rates (11.2±3.7, 80±4.5 at week 4 and 8, respectively) than the poly(L-lactic acid)-HA (PLLA-HA) control group (9.3±2.4 and 71.1±4.4) (p<0.05). The POC-M-click-HA scaffold-fused vertebrates possessed a maximum load and stiffness of 880.8±14.5 N and 843.2±22.4 N/mm, respectively, which were also much higher than those of the PLLA-HA group (maximum: 712.0±37.5 N, stiffness: 622.5±28.4 N/mm, p<0.05). Overall, the results suggest that POC-M-click-HA scaffolds could potentially serve as promising bone grafts for spinal fusion applications. PMID:26213625

Modeling of the dolphin's clicking sound source: The influence of the critical parameters

NASA Astrophysics Data System (ADS)

Dubrovsky, N. A.; Gladilin, A.; Møhl, B.; Wahlberg, M.

2004-07-01

A physical and a mathematical models of the dolphin’s source of echolocation clicks have been recently proposed. The physical model includes a bottle of pressurized air connected to the atmosphere with an underwater rubber tube. A compressing rubber ring is placed on the underwater portion of the tube. The ring blocks the air jet passing through the tube from the bottle. This ring can be brought into self-oscillation by the air jet. In the simplest case, the ring displacement follows a repeated triangular waveform. Because the acoustic pressure gradient is proportional to the second time derivative of the displacement, clicks arise at the bends of the displacement waveform. The mathematical model describes the dipole oscillations of a sphere “frozen” in the ring and calculates the waveform and the sound pressure of the generated clicks. The critical parameters of the mathematical model are the radius of the sphere and the peak value and duration of the triangular displacement curve. This model allows one to solve both the forward (deriving the properties of acoustic clicks from the known source parameters) and the inverse (calculating the source parameters from the acoustic data) problems. Data from click records of Odontocetes were used to derive both the displacement waveforms and the size of the “frozen sphere” or a structure functionally similar to it. The mathematical model predicts a maximum source level of up to 235 dB re 1 μPa at 1-m range when using a 5-cm radius of the “frozen” sphere and a 4-mm maximal displacement. The predicted sound pressure level is similar to that of the clicks produced by Odontocetest.

SureClick® (Darbepoetin alfa) can improve perceived satisfaction and competence for anemia treatment and increase self-administration in non-dialyzed patients with chronic kidney disease.

PubMed

Bonafont, Xavier; Romero, Ramón; Martínez, Isabel; del Pino, María D; Gil, José M; Aranda, Pedro; Roca, Ramón; Claverol, Joana; Cucala, Mercedes

2013-01-01

SureClick® is a prefilled pen for administration of darbepoetin alfa (DA) that is ready-to-use. We explored patient satisfaction with SureClick® compared with prefilled syringes (PFS). Multicenter, prospective, 6-months, observational study in non-dialyzed patients with chronic kidney disease (CKD) treated with DA in PFS who switched to SureClick® at baseline. Main outcomes were: change in Anemia Treatment Satisfaction Questionnaire (ATSQ-S), Perceived Competence for Anemia Scale (PCAS) and self-administration rate. We enrolled 132 patients with a mean(SD) age of 71.3 (14.6) years, 57.6% women. Mean(SD) ATSQ-S scores at baseline and final records were 25.5 (7.9) and 31.6 (4.9) (on a scale from 0 to 36 maximum satisfaction-, mean change: 6.2, 95%CI: 4.6-7.8, p<0.0001). The PCAS also increased significantly (4.3 (2.0) vs 5.6 (1.6), on a scale from 1 to 7 maximum competence, p<0.0001). At baseline 47.7% of patients self-administered DA with PFS, vs 74.2% with SureClick® (p<0.001). No significant changes in hemoglobin were observed (11.4 (0.5) vs 11.6 (1.3) g/dl, p=0.193). Two patients (1.5%) had adverse reactions to SureClick® (pain on application). Our results suggest that the change from PFS to SureClick® could increase patient satisfaction and perceived competence in anemia management in non-dialyzed CKD patients, and could increase the self-administration rate, thereby reducing use of health resources.

Conventional and cross-correlation brain-stem auditory evoked responses in the white leghorn chick: rate manipulations

NASA Technical Reports Server (NTRS)

Burkard, R.; Jones, S.; Jones, T.

1994-01-01

Rate-dependent changes in the chick brain-stem auditory evoked response (BAER) using conventional averaging and a cross-correlation technique were investigated. Five 15- to 19-day-old white leghorn chicks were anesthetized with Chloropent. In each chick, the left ear was acoustically stimulated. Electrical pulses of 0.1-ms duration were shaped, attenuated, and passed through a current driver to an Etymotic ER-2 which was sealed in the ear canal. Electrical activity from stainless-steel electrodes was amplified, filtered (300-3000 Hz) and digitized at 20 kHz. Click levels included 70 and 90 dB peSPL. In each animal, conventional BAERs were obtained at rates ranging from 5 to 90 Hz. BAERs were also obtained using a cross-correlation technique involving pseudorandom pulse sequences called maximum length sequences (MLSs). The minimum time between pulses, called the minimum pulse interval (MPI), ranged from 0.5 to 6 ms. Two BAERs were obtained for each condition. Dependent variables included the latency and amplitude of the cochlear microphonic (CM), wave 2 and wave 3. BAERs were observed in all chicks, for all level by rate combinations for both conventional and MLS BAERs. There was no effect of click level or rate on the latency of the CM. The latency of waves 2 and 3 increased with decreasing click level and increasing rate. CM amplitude decreased with decreasing click level, but was not influenced by click rate for the 70 dB peSPL condition. For the 90 dB peSPL click, CM amplitude was uninfluenced by click rate for conventional averaging. For MLS BAERs, CM amplitude was similar to conventional averaging for longer MPIs.(ABSTRACT TRUNCATED AT 250 WORDS).

Electronic tags and genetics explore variation in migrating steelhead kelts (oncorhynchus mykiss), Ninilchik river, Alaska

USGS Publications Warehouse

Nielsen, J.L.; Turner, S.M.; Zimmerman, C.E.

2011-01-01

Acoustic and archival tags examined freshwater and marine migrations of postspawn steelhead kelts (Oncorhynchus mykiss) in the Ninilchik River, Alaska, USA. Postspawn steelhead were captured at a weir in 2002-2005. Scale analysis indicated multiple migratory life histories and spawning behaviors. Acoustic tags were implanted in 99 kelts (2002-2003), and an array of acoustic receivers calculated the average speed of outmigration, timing of saltwater entry, and duration of residency in the vicinity of the river mouth. Ocean migration data were recovered from two archival tags implanted in kelts in 2004 (one male and one female). Archival tags documented seasonal differences in maximum depth and behavior with both fish spending 97% of time at sea <6 m depth (day and night). All study fish were double tagged with passive integrated transponder (PIT) tags implanted in the body cavity. Less than 4% of PIT tags were retained in postspawn steelhead. Molecular genetics demonstrated no significant differences in genetic population structure across years or among spawning life history types, suggesting a genetically panmictic population with highly diverse life history characteristics in the Ninilchik River.

Lanthanide complexes of azidophenacyl-DO3A as new synthons for click chemistry and the synthesis of heterometallic lanthanide arrays.

PubMed

Tropiano, Manuel; Kenwright, Alan M; Faulkner, Stephen

2015-04-07

Lanthanide complexes of azidophenacyl DO3A are effective substrates for click reactions with ethyne derivatives, giving rise to aryl triazole appended lanthanide complexes, in which the aryl triazole acts as an effective sensitising chromophore for lanthanide luminescence. They also undergo click chemistry with propargylDO3A derivatives, giving rise to heterometallic complexes. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Effect of Broadband Nature of Marine Mammal Echolocation Clicks on Click-Based Population Density Estimates

DTIC Science & Technology

2015-09-30

sonar sound and therefore of high relevance to the US Navy, and sperm whale (Physeter macrocephalus) clicks, whose high source level assures long...beaked whale, and Zimmer (2011) for the sperm whale. 5 For background noise we used measurements obtained with the Delphinus towed array at the US...Atlantic Undersea Test and Evaluation Center (AUTEC), where both Blainville’s beaked whales and sperm whales are commonly present. Analysis of the

A Multi-Week Behavioral Sampling Tag for Sound Effects Studies: Design Trade-Offs and Prototype Evaluation

DTIC Science & Technology

2014-09-30

to establish the performance of algorithms detecting dives, strokes , clicks, respiration and gait changes. We have also found that a combination of...whale click count, total click count, vocal duration, SOC2 depth, EOC3 depth) Descent 40 bits (duration, vertical speed, stroke count 0...100 m, stroke count 100-400 m, OBDA4, sum sr35) Bottom 26 bits (movement index6, OBDA, jerk events7, median jerk depth) Ascent

Click hydrogels, microgels and nanogels: emerging platforms for drug delivery and tissue engineering.

PubMed

Jiang, Yanjiao; Chen, Jing; Deng, Chao; Suuronen, Erik J; Zhong, Zhiyuan

2014-06-01

Hydrogels, microgels and nanogels have emerged as versatile and viable platforms for sustained protein release, targeted drug delivery, and tissue engineering due to excellent biocompatibility, a microporous structure with tunable porosity and pore size, and dimensions spanning from human organs, cells to viruses. In the past decade, remarkable advances in hydrogels, microgels and nanogels have been achieved with click chemistry. It is a most promising strategy to prepare gels with varying dimensions owing to its high reactivity, superb selectivity, and mild reaction conditions. In particular, the recent development of copper-free click chemistry such as strain-promoted azide-alkyne cycloaddition, radical mediated thiol-ene chemistry, Diels-Alder reaction, tetrazole-alkene photo-click chemistry, and oxime reaction renders it possible to form hydrogels, microgels and nanogels without the use of potentially toxic catalysts or immunogenic enzymes that are commonly required. Notably, unlike other chemical approaches, click chemistry owing to its unique bioorthogonal feature does not interfere with encapsulated bioactives such as living cells, proteins and drugs and furthermore allows versatile preparation of micropatterned biomimetic hydrogels, functional microgels and nanogels. In this review, recent exciting developments in click hydrogels, microgels and nanogels, as well as their biomedical applications such as controlled protein and drug release, tissue engineering, and regenerative medicine are presented and discussed. Copyright © 2014 Elsevier Ltd. All rights reserved.

The Source Parameters of Echolocation Clicks from Captive and Free-Ranging Yangtze Finless Porpoises (Neophocaena asiaeorientalis asiaeorientalis)

PubMed Central

Fang, Liang; Wang, Ding; Li, Yongtao; Cheng, Zhaolong; Pine, Matthew K.; Wang, Kexiong; Li, Songhai

2015-01-01

The clicks of Yangtze finless porpoises (Neophocaena asiaeorientalis asiaeorientalis) from 7 individuals in the tank of Baiji aquarium, 2 individuals in a netted pen at Shishou Tian-e-zhou Reserve and 4 free-ranging individuals at Tianxingzhou were recorded using a broadband digital recording system with four element hydrophones. The peak-to-peak apparent source level (ASL_pp) of clicks from individuals at the Baiji aquarium was 167 dB re 1 μPa with mean center frequency of 133 kHz, -3dB bandwidth of 18 kHz and -10 dB duration of 58 μs. The ASL_pp of clicks from individuals at the Shishou Tian-e-zhou Reserve was 180 dB re 1 μPa with mean center frequency of 128 kHz, -3dB bandwidth of 20 kHz and -10 dB duration of 39 μs. The ASL_pp of clicks from individuals at Tianxingzhou was 176 dB re 1 μPa with mean center frequency of 129 kHz, -3dB bandwidth of 15 kHz and -10 dB duration of 48 μs. Differences between the source parameters of clicks among the three groups of finless porpoises suggest these animals adapt to their echolocation signals depending on their surroundings. PMID:26053758

Temporal Ventriloquism Reveals Intact Audiovisual Temporal Integration in Amblyopia.

PubMed

Richards, Michael D; Goltz, Herbert C; Wong, Agnes M F

2018-02-01

We have shown previously that amblyopia involves impaired detection of asynchrony between auditory and visual events. To distinguish whether this impairment represents a defect in temporal integration or nonintegrative multisensory processing (e.g., cross-modal matching), we used the temporal ventriloquism effect in which visual temporal order judgment (TOJ) is normally enhanced by a lagging auditory click. Participants with amblyopia (n = 9) and normally sighted controls (n = 9) performed a visual TOJ task. Pairs of clicks accompanied the two lights such that the first click preceded the first light, or second click lagged the second light by 100, 200, or 450 ms. Baseline audiovisual synchrony and visual-only conditions also were tested. Within both groups, just noticeable differences for the visual TOJ task were significantly reduced compared with baseline in the 100- and 200-ms click lag conditions. Within the amblyopia group, poorer stereo acuity and poorer visual acuity in the amblyopic eye were significantly associated with greater enhancement in visual TOJ performance in the 200-ms click lag condition. Audiovisual temporal integration is intact in amblyopia, as indicated by perceptual enhancement in the temporal ventriloquism effect. Furthermore, poorer stereo acuity and poorer visual acuity in the amblyopic eye are associated with a widened temporal binding window for the effect. These findings suggest that previously reported abnormalities in audiovisual multisensory processing may result from impaired cross-modal matching rather than a diminished capacity for temporal audiovisual integration.

Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation.

PubMed

Burnham-Marusich, Amanda R; Plechaty, Anna M; Berninsone, Patricia M

2014-09-01

Currently, there are few methods to detect differences in posttranslational modifications (PTMs) in a specific manner from complex mixtures. Thus, we developed an approach that combines the sensitivity and specificity of click chemistry with the resolution capabilities of 2D-DIGE. In "Click-DIGE", posttranslationally modified proteins are metabolically labeled with azido-substrate analogs, then size- and charge-matched alkyne-Cy3 or alkyne-Cy5 dyes are covalently attached to the azide of the PTM by click chemistry. The fluorescently-tagged protein samples are then multiplexed for 2DE analysis. Whereas standard DIGE labels all proteins, Click-DIGE focuses the analysis of protein differences to a targeted subset of posttranslationally modified proteins within a complex sample (i.e. specific labeling and analysis of azido glycoproteins within a cell lysate). Our data indicate that (i) Click-DIGE specifically labels azido proteins, (ii) the resulting Cy-protein conjugates are spectrally distinct, and (iii) the conjugates are size- and charge-matched at the level of 2DE. We demonstrate the utility of this approach by detecting multiple differentially expressed glycoproteins between a mutant cell line defective in UDP-galactose transport and the parental cell line. We anticipate that the diversity of azido substrates already available will enable Click-DIGE to be compatible with analysis of a wide range of PTMs. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Ways to suppress click and pop for class D amplifiers

NASA Astrophysics Data System (ADS)

Haishi, Wang; Bo, Zhang; Jiang, Sun

2012-08-01

Undesirable audio click and pop may be generated in a speaker or headphone. Compared to linear (class A/B/AB) amplifiers, class D amplifiers that comprise of an input stage and a modulation stage are more prone to producing click and pop. This article analyzes sources that generate click and pop in class D amplifiers, and corresponding ways to suppress them. For a class D amplifier with a single-ended input, click and pop is likely to be due to two factors. One is from a voltage difference (VDIF) between the voltage of an input capacitance (VCIN) and a reference voltage (VREF) of the input stage, and the other one is from the non-linear switching during the setting up of the bias and feedback voltages/currents (BFVC) of the modulation stage. In this article, a fast charging loop is introduced into the input stage to charge VCIN to roughly near VREF. Then a correction loop further charges or discharges VCIN, substantially equalizing it with VREF. Dummy switches are introduced into the modulation stage to provide switching signals for setting up BFVC, and the power switches are disabled until the BFVC are set up successfully. A two channel single-ended class D amplifier with the above features is fabricated with 0.5 μm Bi-CMOS process. Road test and fast Fourier transform analysis indicate that there is no noticeable click and pop.

Observation of the controlled assembly of preclick components in the in situ click chemistry generation of a chitinase inhibitor

PubMed Central

Hirose, Tomoyasu; Maita, Nobuo; Gouda, Hiroaki; Koseki, Jun; Yamamoto, Tsuyoshi; Sugawara, Akihiro; Nakano, Hirofumi; Hirono, Shuichi; Shiomi, Kazuro; Watanabe, Takeshi; Taniguchi, Hisaaki; Sharpless, K. Barry; Ōmura, Satoshi; Sunazuka, Toshiaki

2013-01-01

The Huisgen cycloaddition of azides and alkynes, accelerated by target biomolecules, termed “in situ click chemistry,” has been successfully exploited to discover highly potent enzyme inhibitors. We have previously reported a specific Serratia marcescens chitinase B (SmChiB)-templated syn-triazole inhibitor generated in situ from an azide-bearing inhibitor and an alkyne fragment. Several in situ click chemistry studies have been reported. Although some mechanistic evidence has been obtained, such as X-ray analysis of [protein]–[“click ligand”] complexes, indicating that proteins act as both mold and template between unique pairs of azide and alkyne fragments, to date, observations have been based solely on “postclick” structural information. Here, we describe crystal structures of SmChiB complexed with an azide ligand and an O-allyl oxime fragment as a mimic of a click partner, revealing a mechanism for accelerating syn-triazole formation, which allows generation of its own distinct inhibitor. We have also performed density functional theory calculations based on the X-ray structure to explore the acceleration of the Huisgen cycloaddition by SmChiB. The density functional theory calculations reasonably support that SmChiB plays a role by the cage effect during the pretranslation and posttranslation states of selective syn-triazole click formation. PMID:24043811

Description and clustering of echolocation signals of Commerson's dolphins (Cephalorhynchus commersonii) in Bahía San Julián, Argentina.

PubMed

Reyes Reyes, M Vanesa; Iñíguez, Miguel A; Hevia, Marta; Hildebrand, John A; Melcón, Mariana L

2015-10-01

Commerson's dolphins (Cephalorhynchus commersonii) inhabit coastal waters of Southern South America and Kerguelen Islands. Limited information exists about the acoustic repertoire of this species in the wild. Here, echolocation signals from free-ranging Commerson's dolphins were recorded in Bahía San Julián, Argentina. Signal parameters were calculated and a cluster analysis was made on 3180 regular clicks. Three clusters were obtained based on peak frequency (129, 137, and 173 kHz) and 3 dB bandwidth (8, 6, and 5 kHz). The 428 buzz clicks were analyzed separately. They consisted of clicks emitted with a median inter-click interval of 3.5 ms, peak frequency at 131 kHz, 3 dB bandwidth of 9 kHz, 10 dB bandwidth of 18 kHz, and duration of 56 μs. Buzz clicks were significantly shorter and with a lower peak frequency and a broader bandwidth than most of the regular clicks. This study provided the first description of different echolocation signals, including on- and off-axis signals, recorded from Commerson's dolphins in the wild, most likely as a result of animals at several distances and orientations to the recording device. This information could be useful while doing passive acoustic monitoring.

Chronological changes in the eighth cranial nerve compound action potential (CAP) in experimental endolymphatic hydrops: the effects of altering the polarity of click sounds.

PubMed

Morizono, Tetsuo; Kondo, Tsuyoshi; Yamano, Takafumi; Miyagi, Morimichi; Shiraishi, Kimio

2009-02-01

Using a guinea pig model of experimental endolymphatic hydrops, click sounds of altered polarity showed different latencies and amplitudes in hydropic compared with normal cochleae. Latency changes appeared as early as 1 week after endolymphatic obstruction. This method can help diagnose endolymphatic hydrops. The goal of the study was to develop an objective electrophysiological diagnosis of endolymphatic hydrops. Endolymphatic hydrops were created surgically in guinea pigs. The latency and the amplitude of the eighth cranial nerve compound action potential (CAP) for click sounds of altered polarity were measured up to 8 weeks after the surgery. At early stages after surgery, the latency for condensation clicks became longer, and at later stages the latencies for both condensation and rarefaction became longer. The discrepancy in the latencies for rarefaction and condensation click sounds (rarefaction minus condensation) became larger by the first week after surgery, but no further discrepancy occurred thereafter. Compared with latency changes, amplitude changes in the CAP were rapid and progressive following surgery, suggesting ongoing damage to hair cells.

Mixed-methods analytic approach for determining potential impacts of vessel noise on sperm whale click behavior.

PubMed

Azzara, Alyson J; von Zharen, Wyndylyn M; Newcomb, Joal J

2013-12-01

The Gulf of Mexico is a center of marine activities from seismic exploration to shipping, drilling, platform installation, lightering, and construction, among others. This analysis explored whether sperm whales respond to the passage of vessels using changes in total number of clicks during vessel passages as a proxy for potential variation in behavior. The data for this analysis were collected in 2001 as part of a larger Littoral Acoustic Demonstration Center project using the Environmental Acoustics Recording System buoys. These buoys were bottom moored, autonomous, and self-recording systems consisting of an omni-directional hydrophone and instrument package. Data from 36 days of continuous acoustic monitoring were recorded at a sampling rate of 11.725 kHz, and produced reliable recordings from 5 Hz to ∼5.8 kHz. Multiple preparatory steps were executed including calibration of an automatic click detector. Results indicate a significant decrease (32%) in the number of clicks detected as a ship approached an area. There were also significantly fewer clicks detected after the vessel passed than before (23%).

Circular DNA by "Bis-Click" Ligation: Template-Independent Intramolecular Circularization of Oligonucleotides with Terminal Alkynyl Groups Utilizing Bifunctional Azides.

PubMed

Yang, Haozhe; Seela, Frank

2016-01-22

A highly effective and convenient "bis-click" strategy was developed for the template-independent circularization of single-stranded oligonucleotides by employing copper(I)-assisted azide-alkyne cycloaddition. Terminal triple bonds were incorporated at both ends of linear oligonucleotides. Alkynylated 7-deaza-2'-deoxyadenosine and 2'-deoxyuridine residues with different side chains were used in solid-phase synthesis with phosphoramidite chemistry. The bis-click ligation of linear 9- to 36-mer oligonucleotides with 1,4-bis(azidomethyl)benzene afforded circular DNA in a simple and selective way; azido modification of the oligonucleotide was not necessary. Short ethynyl side chains were compatible with the circularization of longer oligonucleotides, whereas octadiynyl residues were used for short 9-mers. Compared with linear duplexes, circular bis-click constructs exhibit a significantly increased duplex stability over their linear counterparts. The intramolecular bis-click ligation protocol is not limited to DNA, but may also be suitable for the construction of other macrocycles, such as circular RNAs, peptides, or polysaccharides. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

CARES Foundation

MedlinePlus

... CAH) seeking both male and female adults, aged 18-80. CLICK HERE FOR MORE INFORMATION AND STUDY ... Hyperplasia” for females between the ages of 7-18 years old. CLICK HERE FOR MORE INFORMATION AND ...

Bias in online recruitment and retention of racial and ethnic minority men who have sex with men.

PubMed

Sullivan, Patrick S; Khosropour, Christine M; Luisi, Nicole; Amsden, Matthew; Coggia, Tom; Wingood, Gina M; DiClemente, Ralph J

2011-05-13

The Internet has become an increasingly popular venue for men who have sex with men (MSM) to meet potential sex partners. Given this rapid increase in online sex-seeking among MSM, Internet-based interventions represent an important HIV (human immunodeficiency virus) prevention strategy. Unfortunately, black and Hispanic MSM, who are disproportionately impacted by the HIV epidemic in the United States, have been underrepresented in online research studies. Our objective was to examine and quantify factors associated with underrecruitment and underretention of MSM of color in an online HIV behavioral risk research study of MSM recruited from an online social networking site. Internet-using MSM were recruited through banner advertisements on MySpace.com targeted at men who reported in their MySpace profile their age as at least 18 and their sexual orientation as gay, bisexual, or unsure. Multivariable logistic regression models were used to estimate the odds stratified by race and ethnicity of the MySpace user clicking through the banner advertisement. To characterize survey retention, Kaplan-Meier survival curves and multivariable Cox proportional hazards models identified factors associated with survey dropout. Over 30,000 MySpace users clicked on the study banner advertisements (click-through rate of 0.37%, or 30,599 clicks from 8,257,271 impressions). Black (0.36% or 6474 clicks from 1,785,088 impressions) and Hispanic (0.35% or 8873 clicks from 2,510,434 impressions) MySpace users had a lower click-through rate compared with white (0.48% or 6995 clicks from 1,464,262 impressions) MySpace users. However, black men had increased odds of click-through for advertisements displaying a black model versus a white model (adjusted odds ratio [OR] = 1.83, 95% confidence interval [CI] 1.72 - 1.95), and Hispanic participants had increased odds of click-through when shown an advertisement displaying an Asian model versus a white model (adjusted OR = 1.70, 95% CI 1.62 - 1.79). Of the 9005 men who consented to participate, 6258 (69%) completed the entire survey. Among participants reporting only male sex partners, black non-Hispanic and Hispanic participants were significantly more likely to drop out of the survey relative to white non-Hispanic participants (hazard ratio [HR] = 1.6, 95% CI 1.4 - 1.8 and HR = 1.3, 95% CI 1.1 - 1.4, respectively). Men with a college-level of education were more likely to complete the survey than those with a high-school level of education (HR = 0.8, 95% CI 0.7 - 0.9), while men who self-identified as heterosexual were more likely to drop out of the survey compared with men who self-identified as gay (HR = 2.1, 95% CI 1.1 - 3.7). This analysis identified several factors associated with recruitment and retention of MSM in an online survey. Differential click-through rates and increased survey dropout by MSM of color indicate that methods to recruit and retain black and Hispanic MSM in Internet-based research studies are paramount. Although targeting banner advertisements to MSM of color by changing the racial/ethnic composition of the advertisements may increase click-through, decreasing attrition of these study participants once they are engaged in the survey remains a challenge.

CuAAC-Based Click Chemistry in Self-Healing Polymers.

PubMed

Döhler, Diana; Michael, Philipp; Binder, Wolfgang H

2017-10-17

Click chemistry has emerged as a significant tool for materials science, organic chemistry, and bioscience. Based on the initial concept of Barry Sharpless in 2001, the copper(I)-catalyzed azide/alkyne cycloaddition (CuAAC) reaction has triggered a plethora of chemical concepts for linking molecules and building blocks under ambient conditions, forming the basis for applications in autonomous cross-linking materials. Self-healing systems on the other hand are often based on mild cross-linking chemistries that are able to react either autonomously or upon an external trigger. In the ideal case, self-healing takes place efficiently at low temperatures, independent of the substrate(s) used, by forming strong and stable networks, binding to the newly generated (cracked) interfaces to restore the original material properties. The use of the CuAAC in self-healing systems, most of all the careful design of copper-based catalysts linked to additives as well as the chemical diversity of substrates, has led to an enormous potential of applications of this singular reaction. The implementation of click-based strategies in self-healing systems therefore is highly attractive, as here chemical (and physical) concepts of molecular reactivity, molecular design, and even metal catalysis are connected to aspects of materials science. In this Account, we will show how CuAAC reactions of multivalent components can be used as a tool for self-healing materials, achieving cross-linking at low temperatures (exploiting concepts of autocatalysis or internal chelation within the bulk CuAAC and systematic optimization of the efficiency of the used Cu(I) catalysts). Encapsulation strategies to separate the click components by micro- and nanoencapsulation are required in this context. Consequently, the examples reported here describe chemical concepts to realize more efficient and faster click reactions in self-healing polymeric materials. Thus, enhanced chain diffusion in (hyper)branched polymers, autocatalysis, or internal chelation concepts enable efficient click cross-linking already at 5 °C with a simultaneously reduced amount of Cu(I) catalyst and increased reaction rates, culminating in the first reported self-healing system based on click cycloaddition reactions. Via tailor-made nanocarbon/Cu(I) catalysts we can further improve the click cross-linking reaction in view of efficiency and kinetics, leading to the generation of self-healing graphene-based epoxy nanocomposites. Additionally, we have designed special CuAAC click methods for chemical reporting and visualization systems based on the detection of ruptured capsules via a fluorogenic click reaction, which can be combined with CuAAC cross-linking reactions to obtain simultaneous stress detection and self-healing within polymeric materials. In a similar concept, we have prepared polymeric Cu(I)-biscarbene complexes to detect (mechanical) stress within self-healing polymeric materials via a triggered fluorogenic reaction, thus using a destructive force for a constructive chemical response.

Odontocete Studies on the Pacific Missile Range Facility in July/August 2013: Satellite-Tagging, Photo-Identification, and Passive Acoustic Monitoring

DTIC Science & Technology

2014-05-02

descriptive categories: ə.5 kHz click, 1.5–18 kHz clicks (representative of sperm whales [Physeter macrocephalus]), 12–48 kHz click (representative...beaked whale, sperm whale, small odontocete). In the absence of automatically generated positions, a MMAMMAL tool for manually calculating...was located, there could be days where, as an example, sperm whales were present on BSURE but were not noted. Recordings on the M3R system to improve

Web Syndication in a Multilevel Security Environment

DTIC Science & Technology

2012-03-01

law/blog/03/08/131502.html</link> ... <securitylabel><label>SECRET</label></securitylabel> </item> <item> <title>Excellent Banana Bread Recipe</title...only feed available). 7. Click ‘Unsubscribe from Selected Feeds’ to delete the feed. 50 8. The page should refresh , showing your list of feeds is empty...box. 7. Click ‘Subscribe to Feed,’ to add the feed. 8. The page should refresh , showing an error message from the MLS NEWS READER. 9. Click ‘View

Improving the discoverability, accessibility, and citability of omics datasets: a case report.

PubMed

Darlington, Yolanda F; Naumov, Alexey; McOwiti, Apollo; Kankanamge, Wasula H; Becnel, Lauren B; McKenna, Neil J

2017-03-01

Although omics datasets represent valuable assets for hypothesis generation, model testing, and data validation, the infrastructure supporting their reuse lacks organization and consistency. Using nuclear receptor signaling transcriptomic datasets as proof of principle, we developed a model to improve the discoverability, accessibility, and citability of published omics datasets. Primary datasets were retrieved from archives, processed to extract data points, then subjected to metadata enrichment and gap filling. The resulting secondary datasets were exposed on responsive web pages to support mining of gene lists, discovery of related datasets, and single-click citation integration with popular reference managers. Automated processes were established to embed digital object identifier-driven links to the secondary datasets in associated journal articles, small molecule and gene-centric databases, and a dataset search engine. Our model creates multiple points of access to reprocessed and reannotated derivative datasets across the digital biomedical research ecosystem, promoting their visibility and usability across disparate research communities. © The Author 2016. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

One-Click Data Analysis Software for Science Operations

NASA Astrophysics Data System (ADS)

Navarro, Vicente

2015-12-01

One of the important activities of ESA Science Operations Centre is to provide Data Analysis Software (DAS) to enable users and scientists to process data further to higher levels. During operations and post-operations, Data Analysis Software (DAS) is fully maintained and updated for new OS and library releases. Nonetheless, once a Mission goes into the "legacy" phase, there are very limited funds and long-term preservation becomes more and more difficult. Building on Virtual Machine (VM), Cloud computing and Software as a Service (SaaS) technologies, this project has aimed at providing long-term preservation of Data Analysis Software for the following missions: - PIA for ISO (1995) - SAS for XMM-Newton (1999) - Hipe for Herschel (2009) - EXIA for EXOSAT (1983) Following goals have guided the architecture: - Support for all operations, post-operations and archive/legacy phases. - Support for local (user's computer) and cloud environments (ESAC-Cloud, Amazon - AWS). - Support for expert users, requiring full capabilities. - Provision of a simple web-based interface. This talk describes the architecture, challenges, results and lessons learnt gathered in this project.

National Centers for Environmental Prediction

Science.gov Websites

; at the NOAA/ESRL Rapid Refresh Page [<--click here] See "Current and Forecast Graphics" ; at the NOAA/ESRL High-Resolution Rapid Refresh Page [<--click here] NOAA / National Weather

Low-frequency bias tone suppression of auditory-nerve responses to low-level clicks and tones.

PubMed

Nam, Hui; Guinan, John J

2016-11-01

We used low-frequency "bias" tones (BT's) to explore whether click and tone responses are affected in the same way by cochlear active processes. In nonlinear systems the responses to clicks are not always simply related to the responses to tones. Cochlear amplifier gain depends on the incremental slope of the outer-hair-cell (OHC) stereocilia mechano-electric transduction (MET) function. BTs transiently change the operating-point of OHC MET channels and can suppress cochlear-amplifier gain by pushing OHC METs into low-slope saturation regions. BT effects on single auditory-nerve (AN) fibers have been studied on tone responses but not on click responses. We recorded from AN fibers in anesthetized cats and compared tone and click responses using 50 Hz BTs at 70-120 dB SPL to manipulate OHC stereocilia position. BTs can also excite and thereby obscure the BT suppression. We measured AN-fiber response synchrony to BTs alone so that we could exclude suppression measurements when the BT synchrony might obscure the suppression. BT suppression of low-level tone and click responses followed the traditional pattern of twice-a-BT-cycle suppression with more suppression at one phase than the other. The major suppression phases of most fibers were tightly grouped with little difference between click and tone suppressions, which is consistent with low-level click and tone responses being amplified in the same way. The data are also consistent with the operating point of the OHC MET function varying smoothly from symmetric in the base to offset in the apex, and, in contrast, with the IHC MET function being offset throughout the cochlea. As previously reported, bias-tones presented alone excited AN fibers at one or more phases, a phenomena termed "peak splitting" with most BT excitation phases ∼¼ cycle before or after the major suppression phase. We explain peak splitting as being due to distortion in multiple fluid drives to inner-hair-cell stereocilia. Copyright © 2016 Elsevier B.V. All rights reserved.

Back to the Future: Long-Term Seismic Archives Revisited

NASA Astrophysics Data System (ADS)

Waldhauser, F.; Schaff, D. P.

2007-12-01

Archives of digital seismic data recorded by seismometer networks around the world have grown tremendously over the last several decades helped by the deployment of seismic stations and their continued operation within the framework of monitoring seismic activity. These archives typically consist of waveforms of seismic events and associated parametric data such as phase arrival time picks and the location of hypocenters. Catalogs of earthquake locations are fundamental data in seismology, and even in the Earth sciences in general. Yet, these locations have notoriously low spatial resolution because of errors in both the picks and the models commonly used to locate events one at a time. This limits their potential to address fundamental questions concerning the physics of earthquakes, the structure and composition of the Earth's interior, and the seismic hazards associated with active faults. We report on the comprehensive use of modern waveform cross-correlation based methodologies for high- resolution earthquake location - as applied to regional and global long-term seismic databases. By simultaneous re-analysis of two decades of the digital seismic archive of Northern California, reducing pick errors via cross-correlation and model errors via double-differencing, we achieve up to three orders of magnitude resolution improvement over existing hypocenter locations. The relocated events image networks of discrete faults at seismogenic depths across various tectonic settings that until now have been hidden in location uncertainties. Similar location improvements are obtained for earthquakes recorded at global networks by re- processing 40 years of parametric data from the ISC and corresponding waveforms archived at IRIS. Since our methods are scaleable and run on inexpensive Beowulf clusters, periodic re-analysis of entire archives may thus become a routine procedure to continuously improve resolution in existing catalogs. We demonstrate the role of seismic archives in obtaining the precise location of new events in real-time. Such information has considerable social and economic impact in the evaluation and mitigation of seismic hazards, for example, and highlights the need for consistent long-term seismic monitoring and archiving of records.

Click It or Ticket Evaluation, 2011

DOT National Transportation Integrated Search

2013-05-01

The 2011 Click It or Ticket (CIOT) mobilization followed a typical selective traffic enforcement program (STEP) sequence, involving paid media, earned media, and enforcement. A nationally representative telephone survey indicated that the mobilizatio...

Children's Craniofacial Association

MedlinePlus

... Today Click Here Meet our Kids Click Here Sign up for our Newsletter Do Not Fill This Out Donate Qualified Centers Resources Syndromes Contact Us 13140 Coit Road, Suite 517 , Dallas, TX 75240 800.535.3643 | ...

Cocaine

MedlinePlus

... confidencial Press Room » Multi-Media Library » Image Gallery » Cocaine COCAINE To Save Images: First click on the thumbnail ... your Save in directory and then click Save. Cocaine Crack Cocaine RESOURCE CENTER Controlled Substances Act DEA ...

Types of Glaucoma

MedlinePlus

... Support Groups For more information about area support groups, please click here . NEWSWORTHY Advancing the Understanding for Exfoliation click for info © Copyright 2018 The Glaucoma Foundation. All Rights Reserved. | Site Map Web design by Integra Strategic Technologies

Click It or Ticket Evaluation, 2010

DOT National Transportation Integrated Search

2013-05-01

The 201 Click It or Ticket (CIOT) mobilization followed a typical elective traffic enforcement program TEP) sequence, involving paid media, earned media, and enforcement. A nationally representative telephone survey indicated that the mobilization wa...

Ocean Prediction Center

Science.gov Websites

; Forecasts (Click image for more) Ocean Model Currents Observational Data (Click image for more) Observations University Research Court College Park, Maryland 20740-3818 Phone: 301-683-1520 Fax: 301-683-1501 (SDM), 301

National Centers for Environmental Prediction

Science.gov Websites

Contacts Change Log Events Calendar Numerical Forecast Systems Link to NOAA/ESRL Rapid Refresh page [< ;--click here] Link to NOAA/ESRL High-Resolution Rapid Refresh page [<--click here] NOAA / National

Extraction of pulse repetition intervals from sperm whale click trains for ocean acoustic data mining.

PubMed

Zaugg, Serge; van der Schaar, Mike; Houégnigan, Ludwig; André, Michel

2013-02-01

The analysis of acoustic data from the ocean is a valuable tool to study free ranging cetaceans and anthropogenic noise. Due to the typically large volume of acquired data, there is a demand for automated analysis techniques. Many cetaceans produce acoustic pulses (echolocation clicks) with a pulse repetition interval (PRI) remaining nearly constant over several pulses. Analyzing these pulse trains is challenging because they are often interleaved. This article presents an algorithm that estimates a pulse's PRI with respect to neighboring pulses. It includes a deinterleaving step that operates via a spectral dissimilarity metric. The sperm whale (SW) produces trains with PRIs between 0.5 and 2 s. As a validation, the algorithm was used for the PRI-based identification of SW click trains with data from the NEMO-ONDE observatory that contained other pulsed sounds, mainly from ship propellers. Separation of files containing SW clicks with a medium and high signal to noise ratio from files containing other pulsed sounds gave an area under the receiver operating characteristic curve value of 0.96. This study demonstrates that PRI can be used for the automated identification of SW clicks and that deinterleaving via spectral dissimilarity contributes to algorithm performance.

Classification of echolocation clicks from odontocetes in the Southern California Bight.

PubMed

Roch, Marie A; Klinck, Holger; Baumann-Pickering, Simone; Mellinger, David K; Qui, Simon; Soldevilla, Melissa S; Hildebrand, John A

2011-01-01

This study presents a system for classifying echolocation clicks of six species of odontocetes in the Southern California Bight: Visually confirmed bottlenose dolphins, short- and long-beaked common dolphins, Pacific white-sided dolphins, Risso's dolphins, and presumed Cuvier's beaked whales. Echolocation clicks are represented by cepstral feature vectors that are classified by Gaussian mixture models. A randomized cross-validation experiment is designed to provide conditions similar to those found in a field-deployed system. To prevent matched conditions from inappropriately lowering the error rate, echolocation clicks associated with a single sighting are never split across the training and test data. Sightings are randomly permuted before assignment to folds in the experiment. This allows different combinations of the training and test data to be used while keeping data from each sighting entirely in the training or test set. The system achieves a mean error rate of 22% across 100 randomized three-fold cross-validation experiments. Four of the six species had mean error rates lower than the overall mean, with the presumed Cuvier's beaked whale clicks showing the best performance (<2% error rate). Long-beaked common and bottlenose dolphins proved the most difficult to classify, with mean error rates of 53% and 68%, respectively.

Discovery of novel anti-HIV agents via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry-based approach.

PubMed

Gao, Ping; Sun, Lin; Zhou, Junsu; Li, Xiao; Zhan, Peng; Liu, Xinyong

2016-09-01

In recent years, a variety of new synthetic methodologies and concepts have been proposed in the search for new pharmaceutical lead structures and optimization. Notably, the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry approach has drawn great attention and has become a powerful tool for the generation of privileged medicinal skeletons in the discovery of anti-HIV agents. This is due to the high degree of reliability, complete specificity (chemoselectivity and regioselectivity), mild conditions, and the biocompatibility of the reactants. Herein, the authors describe the progress thus far on the discovery of novel anti-HIV agents via the CuAAC click chemistry-based approach. CuAAC click chemistry is a proven protocol for synthesizing triazole products which could serve as basic pharmacophores, act as replacements of traditional scaffold or substituent modification, be a linker of dual-target or dual-site inhibitors and more for the discovery of novel anti-HIV agents. What's more, it also provides convenience and feasibility for dynamic combinatorial chemistry and in situ screening. It is envisioned that click chemistry will draw more attention and make more contributions in anti-HIV drug discovery in the future.

Testing the odontocete acoustic prey debilitation hypothesis: no stunning results.

PubMed

Benoit-Bird, Kelly J; Au, Whitlow W L; Kastelein, Ronald

2006-08-01

The hypothesis that sounds produced by odontocetes can debilitate fish was examined. The effects of simulated odontocete pulsed signals on three species of fish commonly preyed on by odontocetes were examined, exposing three individuals of each species as well as groups of four fish to a high-frequency click of a bottlenose dolphin [peak frequency (PF) 120 kHz, 213-dB peak-to-peak exposure level (EL)], a midfrequency click modeled after a killer whale's signal (PF 55 kHz, 208-dB EL), and a low-frequency click (PF 18 kHz, 193-dB EL). Fish were held in a 50-cm diameter net enclosure immediately in front of a transducer where their swimming behavior, orientation, and balance were observed with two video cameras. Clicks were presented at constant rates and in graded sweeps simulating a foraging dolphin's "terminal buzz." No measurable change in behavior was observed in any of the fish for any signal type or pulse modulation rate, despite the fact that clicks were at or near the maximum source levels recorded for odontocetes. Based on the results, the hypothesis that acoustic signals of odontocetes alone can disorient or "stun" prey cannot be supported.

Click It or Ticket evaluation : 2008-2009.

DOT National Transportation Integrated Search

2013-06-01

Click It or Ticket (CIOT) mobilizations are intense, short-duration, widely publicized enforcement efforts used to improve seat belt use. Annual national mobilizations have been conducted in May every year since 2003. National and State expenditures ...

76 FR 60511 - Amendment of Marine Safety Manual, Volume III

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-29

...'' in the ``Keyword'' box. Click ``Search,'' and then click on the balloon shape in the ``Actions... Comments'' box, which will then become highlighted in blue. In the ``Keyword'' box, insert ``USCG- 2011...

May 2006 Click It or Ticket seat belt mobilization evaluation

DOT National Transportation Integrated Search

2008-06-01

Click It or Ticket (CIOT) mobilizations are intense, short-duration, seat belt publicity and enforcement programs. The 2006 national mobilization involved approximately $27 million of purchased media. Law enforcement agencies across the nation report...

National Institutes of Health, Office of Research on Women's Health

MedlinePlus

... highlights the importance of factoring sex as a biological variable into every stage of research. Right click ... highlights the importance of factoring sex as a biological variable into every stage of research. Right click ...

Climate Prediction Center: ENSO Diagnostic Discussion

Science.gov Websites

: English Version Spanish Version Adobe PDF Reader (Click icon for Adobe PDF Reader) Word: English Version Spanish Version MS Word Viewer (Click icon for MS Word Viewer) HTML: English Version Spanish Version NOAA

DEA Multimedia Drug Library: Marijuana

MedlinePlus

... confidencial Press Room » Multi-Media Library » Image Gallery » Marijuana MARIJUANA To Save Images: First click on the thumbnail ... Save in directory and then click Save. Indoor Marijuana Grow Indoor Marijuana Grow Loose Marijuana Marinol 10mg ...

Second-Generation Difluorinated Cyclooctynes for Copper-Free Click Chemistry

PubMed Central

2008-01-01

The 1,3-dipolar cycloaddition of azides and activated alkynes has been used for site-selective labeling of biomolecules in vitro and in vivo. While copper catalysis has been widely employed to activate terminal alkynes for [3 + 2] cycloaddition, this method, often termed “click chemistry”, is currently incompatible with living systems because of the toxicity of the metal. We recently reported a difluorinated cyclooctyne (DIFO) reagent that rapidly reacts with azides in living cells without the need for copper catalysis. Here we report a novel class of DIFO reagents for copper-free click chemistry that are considerably more synthetically tractable. The new analogues maintained the same elevated rates of [3 + 2] cycloaddition as the parent compound and were used for imaging glycans on live cells. These second-generation DIFO reagents should expand the use of copper-free click chemistry in the hands of biologists. PMID:18680289

Second-generation difluorinated cyclooctynes for copper-free click chemistry.

PubMed

Codelli, Julian A; Baskin, Jeremy M; Agard, Nicholas J; Bertozzi, Carolyn R

2008-08-27

The 1,3-dipolar cycloaddition of azides and activated alkynes has been used for site-selective labeling of biomolecules in vitro and in vivo. While copper catalysis has been widely employed to activate terminal alkynes for [3 + 2] cycloaddition, this method, often termed "click chemistry", is currently incompatible with living systems because of the toxicity of the metal. We recently reported a difluorinated cyclooctyne (DIFO) reagent that rapidly reacts with azides in living cells without the need for copper catalysis. Here we report a novel class of DIFO reagents for copper-free click chemistry that are considerably more synthetically tractable. The new analogues maintained the same elevated rates of [3 + 2] cycloaddition as the parent compound and were used for imaging glycans on live cells. These second-generation DIFO reagents should expand the use of copper-free click chemistry in the hands of biologists.

Surface grafting of reduced graphene oxide using nanocrystalline cellulose via click reaction

NASA Astrophysics Data System (ADS)

Kabiri, Roya; Namazi, Hassan

2014-07-01

Reduced graphene oxide (RGO) sheet was functionalized with nanocrystalline cellulose (NCC) via click coupling between azide-functionalized graphene oxide (GO-N3) and terminal propargyl-functionalized nanocrystalline cellulose (PG-NCC). First, the reactive azide groups were introduced on the surface of GO with azidation of 2-chloroethyl isocyanate-treated graphene oxide (GO-Cl). Then, the resulted compounds were reacted with PG-NCC utilizing copper-catalyzed azide-alkyne cycloaddition. During the click reaction, GO was simultaneously reduced to graphene. The coupling was confirmed by Fourier transform infrared, Raman, DEPT135, and 13C NMR spectroscopy, and the complete exfoliation of graphene in the NCC matrix was confirmed with X-ray diffraction measurement. The degree of functionalization from the gradual mass loss of RGO-NCC suggests that around 23 mass % has been functionalized covalently. The size of both NCC and GO was found to be in nanometric range, which decreased after click reaction.

Perception time and movement time in dolphin pulsing and whistling

NASA Astrophysics Data System (ADS)

Ridgway, Sam; Carder, Donald

2002-05-01

Auditory/vocal response time was separated into perception time (PT) and movement time (MT) in trials with bottlenose dolphins (Tursiops truncatus)-two males and one female. Pressure catheters accepted into the nasal cavity by each dolphin recorded the pressure increase that preceded sound production. Time from acoustic stimulus onset to onset of pressure rise was recorded as PT (range 57 to 314 ms) and pressure rise onset to dolphin sound onset was recorded as MT (range 63 to 363 ms). Blindfolded dolphins trained to report a target by whistling often responded before completion of their 200- to 800-ms echolocation click trains. Detection of the target, indicated by whistling, before termination of the animal's own click train, suggests that dolphins do not voluntarily respond to each successive click but rather set a rhythm such that each click is emitted about 20 ms after the target echo arrives.

Transformation of temporal sequences in the zebra finch auditory system

PubMed Central

Lim, Yoonseob; Lagoy, Ryan; Shinn-Cunningham, Barbara G; Gardner, Timothy J

2016-01-01

This study examines how temporally patterned stimuli are transformed as they propagate from primary to secondary zones in the thalamorecipient auditory pallium in zebra finches. Using a new class of synthetic click stimuli, we find a robust mapping from temporal sequences in the primary zone to distinct population vectors in secondary auditory areas. We tested whether songbirds could discriminate synthetic click sequences in an operant setup and found that a robust behavioral discrimination is present for click sequences composed of intervals ranging from 11 ms to 40 ms, but breaks down for stimuli composed of longer inter-click intervals. This work suggests that the analog of the songbird auditory cortex transforms temporal patterns to sequence-selective population responses or ‘spatial codes', and that these distinct population responses contribute to behavioral discrimination of temporally complex sounds. DOI: http://dx.doi.org/10.7554/eLife.18205.001 PMID:27897971

Dual-Color Click Beetle Luciferase Heteroprotein Fragment Complementation Assays

PubMed Central

Villalobos, Victor; Naik, Snehal; Bruinsma, Monique; Dothager, Robin S.; Pan, Mei-Hsiu; Samrakandi, Mustapha; Moss, Britney; Elhammali, Adnan; Piwnica-Worms, David

2010-01-01

Summary Understanding the functional complexity of protein interactions requires mapping biomolecular complexes within the cellular environment over biologically-relevant time scales. Herein we describe a novel set of reversible, multicolored heteroprotein complementation fragments based on various firefly and click beetle luciferases that utilize the same substrate, D-luciferin. Luciferase heteroprotein fragment complementation systems enabled dual-color quantification of two discreet pairs of interacting proteins simultaneously or two distinct proteins interacting with a third shared protein in live cells. Using real-time analysis of click beetle green and click beetle red luciferase heteroprotein fragment complementation applied to β-TrCP, an E3-ligase common to the regulation of both β-catenin and IκBα, GSK3β was identified as a novel candidate kinase regulating IκBα processing. These dual-color protein interaction switches may enable directed dynamic analysis of a variety of protein interactions in living cells. PMID:20851351

Fluorescence imaging of chromosomal DNA using click chemistry

NASA Astrophysics Data System (ADS)

Ishizuka, Takumi; Liu, Hong Shan; Ito, Kenichiro; Xu, Yan

2016-09-01

Chromosome visualization is essential for chromosome analysis and genetic diagnostics. Here, we developed a click chemistry approach for multicolor imaging of chromosomal DNA instead of the traditional dye method. We first demonstrated that the commercially available reagents allow for the multicolor staining of chromosomes. We then prepared two pro-fluorophore moieties that served as light-up reporters to stain chromosomal DNA based on click reaction and visualized the clear chromosomes in multicolor. We applied this strategy in fluorescence in situ hybridization (FISH) and identified, with high sensitivity and specificity, telomere DNA at the end of the chromosome. We further extended this approach to observe several basic stages of cell division. We found that the click reaction enables direct visualization of the chromosome behavior in cell division. These results suggest that the technique can be broadly used for imaging chromosomes and may serve as a new approach for chromosome analysis and genetic diagnostics.

Cursor Control Device Test Battery

NASA Technical Reports Server (NTRS)

Holden, Kritina; Sandor, Aniko; Pace, John; Thompson, Shelby

2013-01-01

The test battery was developed to provide a standard procedure for cursor control device evaluation. The software was built in Visual Basic and consists of nine tasks and a main menu that integrates the set-up of the tasks. The tasks can be used individually, or in a series defined in the main menu. Task 1, the Unidirectional Pointing Task, tests the speed and accuracy of clicking on targets. Two rectangles with an adjustable width and adjustable center- to-center distance are presented. The task is to click back and forth between the two rectangles. Clicks outside of the rectangles are recorded as errors. Task 2, Multidirectional Pointing Task, measures speed and accuracy of clicking on targets approached from different angles. Twenty-five numbered squares of adjustable width are arranged around an adjustable diameter circle. The task is to point and click on the numbered squares (placed on opposite sides of the circle) in consecutive order. Clicks outside of the squares are recorded as errors. Task 3, Unidirectional (horizontal) Dragging Task, is similar to dragging a file into a folder on a computer desktop. Task 3 requires dragging a square of adjustable width from one rectangle and dropping it into another. The width of each rectangle is adjustable, as well as the distance between the two rectangles. Dropping the square outside of the rectangles is recorded as an error. Task 4, Unidirectional Path Following, is similar to Task 3. The task is to drag a square through a tunnel consisting of two lines. The size of the square and the width of the tunnel are adjustable. If the square touches any of the lines, it is counted as an error and the task is restarted. Task 5, Text Selection, involves clicking on a Start button, and then moving directly to the underlined portion of the displayed text and highlighting it. The pointing distance to the text is adjustable, as well as the to-be-selected font size and the underlined character length. If the selection does not include all of the underlined characters, or includes non-underlined characters, it is recorded as an error. Task 6, Multi-size and Multi-distance Pointing, presents the participant with 24 consecutively numbered buttons of different sizes (63 to 163 pixels), and at different distances (60 to 80 pixels) from the Start button. The task is to click on the Start button, and then move directly to, and click on, each numbered target button in consecutive order. Clicks outside of the target area are errors. Task 7, Standard Interface Elements Task, involves interacting with standard interface elements as instructed in written procedures, including: drop-down menus, sliders, text boxes, radio buttons, and check boxes. Task completion time is recorded. In Task 8, a circular track is presented with a disc in it at the top. Track width and disc size are adjustable. The task is to move the disc with circular motion within the path without touching the boundaries of the track. Time and errors are recorded. Task 9 is a discrete task that allows evaluation of discrete cursor control devices that tab from target to target, such as a castle switch. The task is to follow a predefined path and to click on the yellow targets along the path.

[Computer graphic display of retinal examination results. Software improving the quality of documenting fundus changes].

PubMed

Jürgens, Clemens; Grossjohann, Rico; Czepita, Damian; Tost, Frank

2009-01-01

Graphic documentation of retinal examination results in clinical ophthalmological practice is often depicted using pictures or in handwritten form. Popular software products used to describe changes in the fundus do not vary much from simple graphic programs that enable to insert, scale and edit basic graphic elements such as: a circle, rectangle, arrow or text. Displaying the results of retinal examinations in a unified way is difficult to achieve. Therefore, we devised and implemented modern software tools for this purpose. A computer program enabling to quickly and intuitively form graphs of the fundus, that can be digitally archived or printed was created. Especially for the needs of ophthalmological clinics, a set of standard digital symbols used to document the results of retinal examinations was developed and installed in a library of graphic symbols. These symbols are divided into the following categories: preoperative, postoperative, neovascularization, retinopathy of prematurity. The appropriate symbol can be selected with a click of the mouse and dragged-and-dropped on the canvas of the fundus. Current forms of documenting results of retinal examinations are unsatisfactory, due to the fact that they are time consuming and imprecise. Unequivocal interpretation is difficult or in some cases impossible. Using the developed computer program a sketch of the fundus can be created much more quickly than by hand drawing. Additionally the quality of the medica documentation using a system of well described and standardized symbols will be enhanced. (1) Graphic symbols used to document the results of retinal examinations are a part of everyday clinical practice. (2) The designed computer program will allow quick and intuitive graphical creation of fundus sketches that can be either digitally archived or printed.

Brain stem auditory potentials evoked by clicks in the presence of high-pass filtered noise in dogs.

PubMed

Poncelet, L; Deltenre, P; Coppens, A; Michaux, C; Coussart, E

2006-04-01

This study evaluates the effects of a high-frequency hearing loss simulated by the high-pass-noise masking method, on the click-evoked brain stem-evoked potentials (BAEP) characteristics in dogs. BAEP were obtained in response to rarefaction and condensation click stimuli from 60 dB normal hearing level (NHL, corresponding to 89 dB sound pressure level) to wave V threshold, using steps of 5 dB in eleven 58 to 80-day-old Beagle puppies. Responses were added, providing an equivalent to alternate polarity clicks, and subtracted, providing the rarefaction-condensation potential (RCDP). The procedure was repeated while constant level, high-pass filtered (HPF) noise was superposed to the click. Cut-off frequencies of the successively used filters were 8, 4, 2 and 1 kHz. For each condition, wave V and RCDP thresholds, and slope of the wave V latency-intensity curve (LIC) were collected. The intensity range at which RCDP could not be recorded (pre-RCDP range) was calculated. Compared with the no noise condition, the pre-RCDP range significantly diminished and the wave V threshold significantly increased when the superposed HPF noise reached the 4 kHz area. Wave V LIC slope became significantly steeper with the 2 kHz HPF noise. In this non-invasive model of high-frequency hearing loss, impaired hearing of frequencies from 8 kHz and above escaped detection through click BAEP study in dogs. Frequencies above 13 kHz were however not specifically addressed in this study.

Biosonar adjustments to target range of echolocating bottlenose dolphins (Tursiops sp.) in the wild.

PubMed

Jensen, F H; Bejder, L; Wahlberg, M; Madsen, P T

2009-04-01

Toothed whales use echolocation to locate and track prey. Most knowledge of toothed whale echolocation stems from studies on trained animals, and little is known about how toothed whales regulate and use their biosonar systems in the wild. Recent research suggests that an automatic gain control mechanism in delphinid biosonars adjusts the biosonar output to the one-way transmission loss to the target, possibly a consequence of pneumatic restrictions in how fast the sound generator can be actuated and still maintain high outputs. This study examines the relationships between target range (R), click intervals, and source levels of wild bottlenose dolphins (Tursiops sp.) by recording regular (non-buzz) echolocation clicks with a linear hydrophone array. Dolphins clicked faster with decreasing distance to the array, reflecting a decreasing delay between the outgoing echolocation click and the returning array echo. However, for interclick intervals longer than 30-40 ms, source levels were not limited by the repetition rate. Thus, pneumatic constraints in the sound-production apparatus cannot account for source level adjustments to range as a possible automatic gain control mechanism for target ranges longer than a few body lengths of the dolphin. Source level estimates drop with reducing range between the echolocating dolphins and the target as a function of 17 log(R). This may indicate either (1) an active form of time-varying gain in the biosonar independent of click intervals or (2) a bias in array recordings towards a 20 log(R) relationship for apparent source levels introduced by a threshold on received click levels included in the analysis.

Now That I Have It, What Can I Do with It?

NASA Astrophysics Data System (ADS)

Holmes, Jon L.

1999-11-01

All JCE subscribers now have access to all areas of JCE Online. As a reader of the print Journal you may be wondering what benefits JCE Online offers you and how you can reap those benefits. Point your WWW browser at jchemed.chem.wisc.edu, login, and follow along. Keep in mind that the three benefits outlined below are those that directly benefit you as a Journal reader. JCE Online contains many other resources that will benefit you as a chemistry teacher. Find an Article, Any Article The JCE Online feature that I perceive to be most beneficial to Journal readers is the ease and speed of finding articles. Finding a particular Journal article or several related articles is quickly and conveniently accomplished by using JCE Index online. Clicking the JCE Index item in the left-hand navigation bar leads to the JCE Index search page. A vanity search for articles that I authored or co-authored (type "holmes j" into the search text field and press Enter) produces a list of the ten most recent articles. A click on one of the articles and another click on the Full Text (.pdf) button (in the page menu bar near the top of the page below the global menu bar) and I am looking at one of my articles just as it appeared in the Journal. Four clicks, nine keystrokes, and 25 seconds (your time may vary)... not bad! Searching the Journal has never been easier than using the online JCE Index. If you remember which issue of the Journal contains the article you are looking for, then that article is never more than six mouse clicks away from the JCE Online Home Page. Of course, this only applies if we have the article online; full text articles begin with the September 1996 issue. The first click is on the Past Issues item in the left-hand navigation bar. If the article is not in the current volume of the Journal (your memory is much better than mine if you remember farther back) then the next click (click two) is on the pop-up list of Journal volumes from which you select the year the article appeared. After the correct volume is selected, use your next click to select the issue by clicking (click three) on one of the issue cover thumbnails. This brings you to the issue Table of Contents where you will probably have to scroll down to find the article (click four). Click the title of the article (click five) to go to the abstract of the article. Click six on the Full Text (.pdf) button in the page menu bar finishes the job. On my computer the six clicks from the JCE Online home page to the full text of an article by Jones et al., "Preparing Preservice Chemistry Teachers for Constructivist Classrooms through Use of Authentic Activities", in the July 1997 issue took 35 seconds including the time required to start up Acrobat Reader; a lot quicker than a trip to the chemistry library and easier even than a trip to the bookshelf across the room! A pop-up list is used to select a volume of the Journal from the Past Issues page. As I mentioned above, only issues since September 1996 have full-text articles available online. Abstracts of articles online go back to July 1995. JCE Index does contain citations to all articles published in the Journal back to Volume 1, Number 1more than 25,700 citations to date. When an online search produces an article that is not available online, you will have to retrieve it the old-fashioned way with a trip to the library or bookshelf. But at least you will know exactly where to look. Supplement Your Print Version You may have noticed a W near the title of some articles, especially laboratory experiments, in the Journal Table of Contents and within the Journal. This W denotes articles that contain online supplementary materials. Such materials are provided by the authors of those articles and may include handouts, assignments, worksheets, procedures, digital video, color illustrations, softwarematerials that you will find beneficial in implementing the idea or laboratory experiment. At JCE Online such articles contain a Supplement button in the page menu bar. For articles with supplementary materials, the Supplement button takes JCE subscribers to the supplement download page. Clicking the Supplement button produces the supplement download page. We attempt to provide supplementary materials as PDF files that are readily downloaded, viewed, and printed using Acrobat Reader. We also take the files in the format provided by the author, which you may find easier to edit for your purposes, and combine them into a single compressed file. This file is available in two forms, one for Windows users and one for Macintosh users. Click the Supplements item in the left-hand navigation bar to find out more about downloading and viewing supplemental materials and for a link to a list of all such materials available at JCE Online. Send Us Your Comments and Suggestions At the bottom of every page at JCE Online is a link to our email address. Do not hesitate to use it to tell us what you think about the Journal and JCE Online. We read all such messages and try to reply to every one. I hope you agree that JCE Online has something to offer you and look forward to hearing from you.

The Click It or Ticket Evaluation, 2013

DOT National Transportation Integrated Search

2016-01-01

The Click It or Ticket (CIOT) high-visibility enforcement (HVE) program has been a primary component of national, State, and local efforts to increase seat belt use, being conducted at least annually since 2003. The 2013 CIOT mobilization included 48...

The functions of sound production in the lined seahorse, Hippocampus erectus, and effects of loud ambient noise on its behavior and physiology in captive environments

NASA Astrophysics Data System (ADS)

Anderson, Paul August

Loud noise in aquaria represents a cacophonous environment for captive fishes. I tested the effects of loud noise on acoustic communication, feeding behavior, courtship behavior, and the stress response of the lined seahorse, Hippocampus erectus. Total Root Mean Square (RMS) power of ambient noise to which seahorses are exposed in captivity varies widely but averages 126.1 +/- 0.8 deciBels with reference to one micropascal (dB re: 1 muPa) at the middle of the water column and 133.7 +/- 1.1 dB at the tank bottom, whereas ambient noise in the wild averages 119.6 +/- 3.5 dB. Hearing sensitivity of H. erectus, measured from auditory evoked potentials, demonstrated maximum spectrum-level sensitivities of 105.0 +/- 1.5 dB and 3.5 x 10-3 + 7.6 x 10-4 m/s2 at 200 Hz; which is characteristic of hearing generalists. H. erectus produces acoustic clicks with mean peak spectrum-level amplitudes of 94.3 +/- 0.9 dB at 232 +/- 16 Hz and 1.5 x 10 -3 +/- 1.9 x 10-4 m/s2 at 265 +/- 22 Hz. Frequency matching of clicks to best hearing sensitivity, and estimates of audition of broadband signals suggest that seahorses may hear conspecific clicks, especially in terms of particle motion. Behavioral investigations revealed that clicking did not improve prey capture proficiency. However, animals clicked more often as time progressed in a courtship sequence, and mates performed more courtship behaviors with control animals than with muted animals, lending additional evidence to the role of clicking as an acoustic signal during courtship. Despite loud noise and the role of clicking in communication, masking of the acoustic signal was not demonstrated. Seahorses exposed to loud noise in aquaria for one month demonstrated physiological, chronic stress responses: reduced weight and body condition, and increased heterophil to lymphocyte ratio. Behavioral alterations were characterized by greater mean and variance of activity among animals housed in loud tanks in the first week, followed by habituation. By week four, animals in loud tanks demonstrated variable performance of clicking and piping, putative distress behaviors. Despite the physiological stress response, animals in loud tanks did not reduce feeding response or courtship behavior, suggesting allostasis.

Functionalization of Mechanochemically Passivated Germanium Nanoparticles via "Click" Chemistry

NASA Astrophysics Data System (ADS)

Purkait, Tapas Kumar

Germanium nanoparticles (Ge NPs) may be fascinating for their electronic and optoelectronic properties, as the band gap of Ge NPs can be tuned from the infrared into the visible range of solar spectru. Further functionalization of those nanoparticles may potentially lead to numerous applications ranging from surface attachment, bioimaging, drug delivery and nanoparticles based devices. Blue luminescent germanium nanoparticles were synthesized from a novel top-down mechanochemical process using high energy ball milling (HEBM) of bulk germanium. Various reactive organic molecules (such as, alkynes, nitriles, azides) were used in this process to react with fresh surface and passivate the surface through Ge-C or Ge-N bond. Various purification process, such as gel permeation chromatography (GPC), Soxhlet dailysis etc. were introduced to purify nanoparticles from molecular impurities. A size separation technique was developed using GPC. The size separated Ge NPs were characterize by TEM, small angle X-ray scattering (SAXS), UV-vis absorption and photoluminescence (PL) emission spectroscopy to investigate their size selective properties. Germanium nanoparticles with alkyne termini group were prepared by HEBM of germanium with a mixture of n-alkynes and alpha, o-diynes. Additional functionalization of those nanoparticles was achieved by copper(I) catalyzed azide-alkyne "click" reaction. A variety of organic and organometallic azides including biologically important glucals have been reacted in this manner resulting in nanopartilces adorned with ferrocenyl, trimethylsilyl, and glucal groups. Additional functionalization of those nanoparticles was achieved by reactions with various azides via a Cu(I) catalyzed azide-alkyne "click" reaction. Various azides, including PEG derivatives and cylcodextrin moiety, were grafted to the initially formed surface. Globular nanoparticle arrays were formed through interparticle linking via "click" chemistry or "host-guest" chemistry. Copper(I) catalyzed "click" chemistry also can be explored with azido-terminated Ge NPs which were synthesized by azidation of chloro-terminated Ge NPs. Water soluble PEGylated Ge NPs were synthesized by "click" reaction for biological application. PEGylated Ge NP clusters were prepared using alpha, o-bis alkyno or bis-azido polyethylene glycol (PEG) derivatives by copper catalyzed "click" reaction via inter-particle linking. These nanoparticles were further functionalized by azido beta-cyclodextrin (beta-CD) and azido adamantane via alkyne-azide "click" reactions. Nanoparticle clusters were made from the functionalized Ge NPs by "host-guest" chemistry of beta-CD functionalized Ge NPs either with adamantane functionalized Ge NPs or fullerene, C60.

May 2004 Click It or Ticket seat belt mobilization evaluation : final report

DOT National Transportation Integrated Search

2007-05-01

"Click It or Ticket" (CIOT) is an intense, short duration, seat belt high visibility publicity and enforcement program. The CIOT May 2004 Mobilization involved approximately $32 million dollars of purchased media. Law enforcement agencies in 50 State...

Evaluation of the May 2007 : click it or ticket mobilization.

DOT National Transportation Integrated Search

2009-12-01

Click It or Ticket (CIOT) programs are short-duration, high-visibility enforcement of seat belt laws. The first : nationwide program was conducted in May 2003 followed by similar programs during May of each succeeding : year. This report examines the...

NHTSA Region IV Click It or Ticket campaign, May 2001

DOT National Transportation Integrated Search

2002-01-01

During May 2001, all eight southeastern states in National Highway Traffic Safety Administration Region IV conducted "Click It or Ticket", an intensive belt use enforcement program. The objective of the present study was to evaluate the results of th...

May 2003 Click It or Ticket safety belt mobilization evaluation

DOT National Transportation Integrated Search

2004-03-01

Click It or Ticket (CIOT) is an intense, short duration, safety belt paid advertising and high visibility enforcement program. The CIOT May 2003 Mobilization involved nearly 25 million dollars of purchased media. Law enforcement agencies in 44 of 45 ...

Evaluation of Click It or Ticket model programs

DOT National Transportation Integrated Search

2002-09-01

Click It or Ticket (CIOT) is an intense, short duration, seat belt enforcement program which relies heavily on paid media to reach all motorists. Ten states that implemented CIOT during May 2002 were compared with four states that conducted belt use ...

Navigation To and From a Page: Which Links Get Clicked From Where

EPA Pesticide Factsheets

Use Google Analytics navigation summary data to find out what page users most frequently click your Contact Us link from (Previous Page Path), or which links on your homepage are popular or unpopular (Next Page Path).

76 FR 73998 - Drawbridge Operation Regulations; Gulf Intracoastal Waterway (Algiers Alternate Route), Belle...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-30

...,'' click ``Search,'' and then click on the balloon shape in the ``Actions'' column. If you submit your... accordingly as there is an alternate route. The vertical clearance of the bridge is 40 feet above mean high...

The 2007 click it or ticket high-visibility seat belt mobilization : traffic tech.

DOT National Transportation Integrated Search

2010-09-01

In May 2007 the National Highway Traffic Safety Administration : sponsored the fifth national Click It or Ticket (CIOT) : high-visibility seat belt enforcement mobilization, which followed : the CIOT program model of earned and paid media : publicizi...

Evaluation of Kentucky's "Click It or Ticket" 2008 campaign.

DOT National Transportation Integrated Search

2008-07-01

The objective of this report was to document the results of the "Click It or Ticket" 2008 campaign in Kentucky. The campaign involved a combination of earned media, paid media, and enforcement. : The evaluation of the campaign included documenting th...

Evaluation of Kentucky's Click it or Ticket campaign.

DOT National Transportation Integrated Search

2001-08-01

The objective of this report was to document the results of the Click It or Ticket campaign in Kentucky. The campaign involved a combination of earned media, paid media, and enforcement. : The evaluation of the campaign included documenting the activ...

VeriClick: an efficient tool for table format verification

NASA Astrophysics Data System (ADS)

Nagy, George; Tamhankar, Mangesh

2012-01-01

The essential layout attributes of a visual table can be defined by the location of four critical grid cells. Although these critical cells can often be located by automated analysis, some means of human interaction is necessary for correcting residual errors. VeriClick is a macro-enabled spreadsheet interface that provides ground-truthing, confirmation, correction, and verification functions for CSV tables. All user actions are logged. Experimental results of seven subjects on one hundred tables suggest that VeriClick can provide a ten- to twenty-fold speedup over performing the same functions with standard spreadsheet editing commands.

Compensating for the effects of site and equipment variation on delphinid species identification from their echolocation clicks.

PubMed

Roch, Marie A; Stinner-Sloan, Johanna; Baumann-Pickering, Simone; Wiggins, Sean M

2015-01-01

A concern for applications of machine learning techniques to bioacoustics is whether or not classifiers learn the categories for which they were trained. Unfortunately, information such as characteristics of specific recording equipment or noise environments can also be learned. This question is examined in the context of identifying delphinid species by their echolocation clicks. To reduce the ambiguity between species classification performance and other confounding factors, species whose clicks can be readily distinguished were used in this study: Pacific white-sided and Risso's dolphins. A subset of data from autonomous acoustic recorders located at seven sites in the Southern California Bight collected between 2006 and 2012 was selected. Cepstral-based features were extracted for each echolocation click and Gaussian mixture models were used to classify groups of 100 clicks. One hundred Monte-Carlo three-fold experiments were conducted to examine classification performance where fold composition was determined by acoustic encounter, recorder characteristics, or recording site. The error rate increased from 6.1% when grouped by acoustic encounter to 18.1%, 46.2%, and 33.2% for grouping by equipment, equipment category, and site, respectively. A noise compensation technique reduced error for these grouping schemes to 2.7%, 4.4%, 6.7%, and 11.4%, respectively, a reduction in error rate of 56%-86%.

Hearing sensitivity during target presence and absence while a whale echolocates.

PubMed

Supin, Alexander Ya; Nachtigall, Paul E; Breese, Marlee

2008-01-01

Hearing sensitivity was measured in a false killer whale during echolocation. Sensitivity was measured using probe stimuli as sinusoidally amplitude modulated signals with a 22.5-kHz carrier frequency and recording auditory evoked potentials as envelope-following responses. The probes were presented and responses were recorded during short 2-s periods when the animal echolocated to detect the presence or absence of a target in a go/no-go paradigm. In the target-absent trials, a hearing threshold of 90.4 dB re 1 muPa was found; in the target-present trials, the threshold was 109.8 dB. Thus, a 19.4-dB difference was found between thresholds in the target-present and target-absent trials. To check the possibility that this difference was the result of different masking degree of the probe by the emitted sonar clicks, click statistics were investigated in similar trials. No indication was found that the energy of the emitted clicks was higher in the target-present than in target-absent trials; on the contrary, mean click level, mean number of clicks per train, and overall train energy was slightly higher in the target-absent trials. Thus the data indicate that the hearing sensitivity of the whale varied depending on target presence or absence.

The Starchive: An open access, open source archive of nearby and young stars and their planets

NASA Astrophysics Data System (ADS)

Tanner, Angelle; Gelino, Chris; Elfeki, Mario

2015-12-01

Historically, astronomers have utilized a piecemeal set of archives such as SIMBAD, the Washington Double Star Catalog, various exoplanet encyclopedias and electronic tables from the literature to cobble together stellar and exo-planetary parameters in the absence of corresponding images and spectra. As the search for planets around young stars through direct imaging, transits and infrared/optical radial velocity surveys blossoms, there is a void in the available set of to create comprehensive lists of the stellar parameters of nearby stars especially for important parameters such as metallicity and stellar activity indicators. For direct imaging surveys, we need better resources for downloading existing high contrast images to help confirm new discoveries and find ideal target stars. Once we have discovered new planets, we need a uniform database of stellar and planetary parameters from which to look for correlations to better understand the formation and evolution of these systems. As a solution to these issues, we are developing the Starchive - an open access stellar archive in the spirit of the open exoplanet catalog, the Kepler Community Follow-up Program and many others. The archive will allow users to download various datasets, upload new images, spectra and metadata and will contain multiple plotting tools to use in presentations and data interpretations. While we will highly regulate and constantly validate the data being placed into our archive the open nature of its design is intended to allow the database to be expanded efficiently and have a level of versatility which is necessary in today's fast moving, big data community. Finally, the front-end scripts will be placed on github and users will be encouraged to contribute new plotting tools. Here, I will introduce the community to the content and expected capabilities of the archive and query the audience for community feedback.

High-Yield Spin Labeling of Long RNAs for Electron Paramagnetic Resonance Spectroscopy.

PubMed

Kerzhner, Mark; Matsuoka, Hideto; Wuebben, Christine; Famulok, Michael; Schiemann, Olav

2018-05-10

Site-directed spin labeling is a powerful tool for investigating the conformation and dynamics of biomacromolecules such as RNA. Here we introduce a spin labeling strategy based on click chemistry in solution that, in combination with enzymatic ligation, allows highly efficient labeling of complex and long RNAs with short reaction times and suppressed RNA degradation. With this approach, a 34-nucleotide aptamer domain of the preQ1 riboswitch and an 81-nucleotide TPP riboswitch aptamer could be labeled with two labels in several positions. We then show that conformations of the preQ1 aptamer and its dynamics can be monitored in the absence and presence of Mg 2+ and a preQ1 ligand by continuous wave electron paramagnetic resonance spectroscopy at room temperature and pulsed electron-electron double resonance spectroscopy (PELDOR or DEER) in the frozen state.

Bistetrazine-cyanines as double-clicking fluorogenic two-point binder or crosslinker probes.

PubMed

Kormos, Attila; Koehler, Christine; Fodor, Eszter; Rutkai, Zsófia; Martin, Maddison; Mező, Gábor; Lemke, Edward; Kele, Péter

2018-04-20

Fluorogenic probes are capable of minimizing background fluorescence of unreacted and non-specifically adsorbed reagents. The preceding years have brought substantial developments in the design and synthesis of bioorthogonally applicable fluorogenic systems mainly based on the quenching effects of azide and tetrazine moieties. The modulation power exerted by these bioorthogonal motifs typically becomes less efficient on more conjugated systems, i.e. on probes with red-shifted emission wavelength. In order to reach efficient quenching, i.e. fluorogenicity even in the red range of the spectrum, We present the synthesis, fluorogenic and conjugation characterization of bistetrazine-cyanine probes with emission maxima between 600-620 nm. The probes can bind to genetically altered proteins harboring an 11-amino acid peptide tag with two appending cyclooctyne motifs. Moreover, we also demonstrate the use of these bistetrazines as fluorogenic, covalent cross-linkers between monocyclooctynylated proteins. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Modified glycogen as construction material for functional biomimetic microfibers.

PubMed

Rabyk, Mariia; Hruby, Martin; Vetrik, Miroslav; Kucka, Jan; Proks, Vladimir; Parizek, Martin; Konefal, Rafal; Krist, Pavel; Chvatil, David; Bacakova, Lucie; Slouf, Miroslav; Stepanek, Petr

2016-11-05

We describe a conceptually new, microfibrous, biodegradable functional material prepared from a modified storage polysaccharide also present in humans (glycogen) showing strong potential as direct-contact dressing/interface material for wound healing. Double bonds were introduced into glycogen via allylation and were further exploited for crosslinking of the microfibers. Triple bonds were introduced by propargylation and served for further click functionalization of the microfibers with bioactive peptide. A simple solvent-free method allowing the preparation of thick layers was used to produce microfibers (diameter ca 2μm) from allylated and/or propargylated glycogen. Crosslinking of the samples was performed by microtron beta-irradiation, and the irradiation dose was optimized to 2kGy. The results from biological testing showed that these highly porous, hydrophilic, readily functionalizable materials were completely nontoxic to cells growing in their presence. The fibers were gradually degraded in the presence of cells. Copyright © 2016 Elsevier Ltd. All rights reserved.

Surface immobilization of heparin on functional polyisobutylene-based thermoplastic elastomer as a potential artificial vascular graft

NASA Astrophysics Data System (ADS)

Wu, Yi-Bo; Li, Kang; Xiang, Dong; Zhang, Min; Yang, Dan; Zhang, Jin-Han; Mao, Jing; Wang, Hao; Guo, Wen-Li

2018-07-01

Polyisobutylene-based thermoplastic elastomer (TPE) is a new soft biomaterial. Hydroxyl functional dendritic polyisobutylene-based TPEs (arb-SIBS-OH), which satisfy the design requirements for small-diameter vascular substitutes, were synthesized by controlled carbocationic polymerization. Creep property, which is the destructive weakness of polyisobutylene-based TPEs, was significantly improved with the formation of a "double network" promoted by branched structure and microphase separation. Compatibility of arb-SIBS-OH with rabbit blood was markedly enhanced by modifying heparin grafted from these hydroxyl functional groups. Application of "click chemistry" to immobilize heparin on arb-SIBS-OH surface was apparently effective in enhancing the bioactivity of heparin. Immobilized heparin, which directly bonded by ester bonds, was more likely to form multi-point binding on arb-SIBS-OH surface. This process hindered the accessibility of the heparin active sequence to antithrombin.

LOFAR discovery of a double radio halo system in Abell 1758 and radio/X-ray study of the cluster pair

NASA Astrophysics Data System (ADS)

Botteon, A.; Shimwell, T. W.; Bonafede, A.; Dallacasa, D.; Brunetti, G.; Mandal, S.; van Weeren, R. J.; Brüggen, M.; Cassano, R.; de Gasperin, F.; Hoang, D. N.; Hoeft, M.; Röttgering, H. J. A.; Savini, F.; White, G. J.; Wilber, A.; Venturi, T.

2018-05-01

Radio halos and radio relics are diffuse synchrotron sources that extend over Mpc-scales and are found in a number of merger galaxy clusters. They are believed to form as a consequence of the energy that is dissipated by turbulence and shocks in the intra-cluster medium (ICM). However, the precise physical processes that generate these steep synchrotron spectrum sources are still poorly constrained. We present a new LOFAR observation of the double galaxy cluster Abell 1758. This system is composed of A1758N, a massive cluster hosting a known giant radio halo, and A1758S, which is a less massive cluster whose diffuse radio emission is confirmed here for the first time. Our observations have revealed a radio halo and a candidate radio relic in A1758S, and a suggestion of emission along the bridge connecting the two systems which deserves confirmation. We combined the LOFAR data with archival VLA and GMRT observations to constrain the spectral properties of the diffuse emission. We also analyzed a deep archival Chandra observation and used this to provide evidence that A1758N and A1758S are in a pre-merger phase. The ICM temperature across the bridge that connects the two systems shows a jump which might indicate the presence of a transversal shock generated in the initial stage of the merger.

Test Report for Cesium and Solids Removal from an 11.5L Composite of Archived Hanford Double Shell Tank Supernate for Off-Site Disposal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Doll, Stephanie R.; Cooke, Gary A.

The 222-S Laboratory blended supernate waste from Hanford Tanks 241-AN-101, 241-AN- 106, 241-AP-105, 241-AP-106, 241-AP-107, and 241-AY-101 from the hot cell archive to create a bulk composite. The composite was blended with 600 mL 19.4 M NaOH, which brought the total volume to approximately 11.5 L (3 gal). The composite was filtered to remove solids and passed through spherical resorcinol-formaldehyde ion-exchange resin columns to remove cesium. The composite masses were tracked as a treatability study. Samples collected before, during, and after the ion-exchange process were characterized for a full suite of analytes (inorganic, organic, and radionuclides) to aid in themore » classification of the waste for shipping, receiving, treatment, and disposal determinations.« less

Test Report for Cesium and Solids Removal from an 11.5L Composite of Archived Hanford Double Shell Tank Supernate for Off-Site Disposal.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Doll, S. R.; Cooke, G. A.

The 222-S Laboratory blended supernate waste from Hanford Tanks 241-AN-101, 241-AN- 106, 241-AP-105, 241-AP-106, 241-AP-107, and 241-AY-101 from the hot cell archive to create a bulk composite. The composite was blended with 600 mL 19.4 M NaOH, which brought the total volume to approximately 11.5 L (3 gal). The composite was filtered to remove solids and passed through spherical resorcinol-formaldehyde ion-exchange resin columns to remove cesium. The composite masses were tracked as a treatability study. Samples collected before, during, and after the ion exchange process were characterized for a full suite of analytes (inorganic, organic, and radionuclides) to aid inmore » the classification of the waste for shipping, receiving, treatment, and disposal determinations.« less

Architecture with GIDEON, A Program for Design in Structural DNA Nanotechnology

PubMed Central

Birac, Jeffrey J.; Sherman, William B.; Kopatsch, Jens; Constantinou, Pamela E.; Seeman, Nadrian C.

2012-01-01

We present geometry based design strategies for DNA nanostructures. The strategies have been implemented with GIDEON – a Graphical Integrated Development Environment for OligoNucleotides. GIDEON has a highly flexible graphical user interface that facilitates the development of simple yet precise models, and the evaluation of strains therein. Models are built on a simple model of undistorted B-DNA double-helical domains. Simple point and click manipulations of the model allow the minimization of strain in the phosphate-backbone linkages between these domains and the identification of any steric clashes that might occur as a result. Detailed analysis of 3D triangles yields clear predictions of the strains associated with triangles of different sizes. We have carried out experiments that confirm that 3D triangles form well only when their geometrical strain is less than 4% deviation from the estimated relaxed structure. Thus geometry-based techniques alone, without energetic considerations, can be used to explain general trends in DNA structure formation. We have used GIDEON to build detailed models of double crossover and triple crossover molecules, evaluating the non-planarity associated with base tilt and junction mis-alignments. Computer modeling using a graphical user interface overcomes the limited precision of physical models for larger systems, and the limited interaction rate associated with earlier, command-line driven software. PMID:16630733

Online Advertising to Reach and Recruit Latino Smokers to an Internet Cessation Program: Impact and Costs

PubMed Central

Fang, Ye; Moreno, Jose L; Streiff, Shawn L; Villegas, Jorge; Muñoz, Ricardo F; Tercyak, Kenneth P; Mandelblatt, Jeanne S; Vallone, Donna M

2012-01-01

Background Tobacco cessation among Latinos is a public health priority in the United States, particularly given the relatively high growth of this population segment. Although a substantial percentage of American Latinos use the Internet, they have not engaged in Web-based cessation programs as readily as other racial/ethnic subgroups. A lack of culturally specific advertising efforts may partly explain this disparity. Objective Phase I of this study focused on the development of four Spanish-language online banner advertisements to promote a free Spanish-language smoking cessation website (es.BecomeAnEX.org). Phase II examined the relative effectiveness of the four banner ads in reaching and recruiting Latino smokers to the cessation website. Methods In Phase I, 200 Spanish-speaking Latino smokers completed an online survey to indicate their preference for Spanish-language banner ads that incorporated either the cultural value of family (familismo) or fatalism (fatalismo). Ads included variations on message framing (gain vs loss) and depth of cultural targeting (surface vs deep). In Phase II, a Latin square design evaluated the effectiveness of the four preferred ads from Phase I. Ads were systematically rotated across four popular Latino websites (MySpace Latino, MSN Latino, MiGente, and Yahoo! en Español) over four months from August to November 2009. Tracking software recorded ad clicks and registrants on the cessation website. Negative binomial regression and general linear modeling examined the main and interacting effects of message framing and depth of cultural targeting for four outcomes: number of clicks, click-through rate, number of registrants, and cost per registrant. Results In Phase I, smokers preferred the four ads featuring familismo. In Phase II, 24,829,007 impressions were placed, yielding 24,822 clicks, an overall click-through rate of 0.10%, and 500 registrants (2.77% conversion rate). Advertising costs totaled US $104,669.49, resulting in an overall cost per click of US $4.22 and cost per registrant of US $209.34. Website placement predicted all four outcomes (all P values < .01). Yahoo! en Español yielded the highest click-through rate (0.167%) and number of registrants (n = 267). The message framing and cultural targeting interaction was not significant. Contrary to hypotheses, loss-framed ads yielded a higher click-through rate than gain-framed ads (point estimate = 1.08, 95% CI 1.03 1.14, P = 0.004), and surface-targeted ads outperformed deep-targeted ads for clicks (point estimate = 1.20, 95% CI 1.13 1.28, P < .001), click-through rate (point estimate = 1.22, 95% CI 1.16 1.29, P < .001), and number of registrants (point estimate = 2.73, 95% CI 2.14 3.48, P < .001). Conclusions Online advertising can be an effective and cost-efficient strategy to reach and engage Spanish-speaking Latino smokers in an evidence-based Internet cessation program. Cultural targeting and smoking-relevant images may be important factors for banner ad design. Online advertising holds potential for Web-based cessation program implementation and research. PMID:22954502

Online advertising to reach and recruit Latino smokers to an internet cessation program: impact and costs.

PubMed

Graham, Amanda L; Fang, Ye; Moreno, Jose L; Streiff, Shawn L; Villegas, Jorge; Muñoz, Ricardo F; Tercyak, Kenneth P; Mandelblatt, Jeanne S; Vallone, Donna M

2012-08-27

Tobacco cessation among Latinos is a public health priority in the United States, particularly given the relatively high growth of this population segment. Although a substantial percentage of American Latinos use the Internet, they have not engaged in Web-based cessation programs as readily as other racial/ethnic subgroups. A lack of culturally specific advertising efforts may partly explain this disparity. Phase I of this study focused on the development of four Spanish-language online banner advertisements to promote a free Spanish-language smoking cessation website (es.BecomeAnEX.org). Phase II examined the relative effectiveness of the four banner ads in reaching and recruiting Latino smokers to the cessation website. In Phase I, 200 Spanish-speaking Latino smokers completed an online survey to indicate their preference for Spanish-language banner ads that incorporated either the cultural value of family (familismo) or fatalism (fatalismo). Ads included variations on message framing (gain vs loss) and depth of cultural targeting (surface vs deep). In Phase II, a Latin square design evaluated the effectiveness of the four preferred ads from Phase I. Ads were systematically rotated across four popular Latino websites (MySpace Latino, MSN Latino, MiGente, and Yahoo! en Español) over four months from August to November 2009. Tracking software recorded ad clicks and registrants on the cessation website. Negative binomial regression and general linear modeling examined the main and interacting effects of message framing and depth of cultural targeting for four outcomes: number of clicks, click-through rate, number of registrants, and cost per registrant. In Phase I, smokers preferred the four ads featuring familismo. In Phase II, 24,829,007 impressions were placed, yielding 24,822 clicks, an overall click-through rate of 0.10%, and 500 registrants (2.77% conversion rate). Advertising costs totaled US $104,669.49, resulting in an overall cost per click of US $4.22 and cost per registrant of US $209.34. Website placement predicted all four outcomes (all P values < .01). Yahoo! en Español yielded the highest click-through rate (0.167%) and number of registrants (n = 267). The message framing and cultural targeting interaction was not significant. Contrary to hypotheses, loss-framed ads yielded a higher click-through rate than gain-framed ads (point estimate = 1.08, 95% CI 1.03 1.14, P = 0.004), and surface-targeted ads outperformed deep-targeted ads for clicks (point estimate = 1.20, 95% CI 1.13 1.28, P < .001), click-through rate (point estimate = 1.22, 95% CI 1.16 1.29, P < .001), and number of registrants (point estimate = 2.73, 95% CI 2.14 3.48, P < .001). Online advertising can be an effective and cost-efficient strategy to reach and engage Spanish-speaking Latino smokers in an evidence-based Internet cessation program. Cultural targeting and smoking-relevant images may be important factors for banner ad design. Online advertising holds potential for Web-based cessation program implementation and research.

Flipping & Clicking Your Way to Higher-Order Learning

ERIC Educational Resources Information Center

Garver, Michael S.; Roberts, Brian A.

2013-01-01

This innovative system of teaching and learning includes the implementation of two effective learning technologies: podcasting ("flipping") and classroom response systems ("clicking"). Students watch lectures in podcast format before coming to class, which allows the "entire" class period to be devoted to active…

The Click It or Ticket evaluation, 2012 : traffic safety facts : research note.

DOT National Transportation Integrated Search

2014-03-01

Click It or Ticket (CIOT) mobilizations are national, State, and local high-visibility enforcement (HVE) efforts designed to encourage motorists to buckle up. They have been conducted at the national level each year around Memorial Day since 2003. : ...

Signals from the deep: Spatial and temporal acoustic occurrence of beaked whales off western Ireland.

PubMed

Kowarski, Katie; Delarue, Julien; Martin, Bruce; O'Brien, Joanne; Meade, Rossa; Ó Cadhla, Oliver; Berrow, Simon

2018-01-01

Little is known of the spatio-temporal occurrence of beaked whales off western Ireland, limiting the ability of Regulators to implement appropriate management and conservation measures. To address this knowledge gap, static acoustic monitoring was carried out using eight fixed bottom-mounted autonomous acoustic recorders: four from May to December 2015 on Ireland's northern slope and four from March to November 2016 on the western and southern slopes. Recorders ran for 205 to 230 days, resulting in 4.09 TB of data sampled at 250 kHz which could capture beaked whale acoustic signals. Zero-crossing-based automated detectors identified beaked whale clicks. A sample of detections was manually validated to evaluate and optimize detector performance. Analysis confirmed the occurrence of Sowerby's and Cuvier's beaked whales and Northern bottlenose whales. Northern bottlenose whale clicks occurred in late summer and autumn, but were too few to allow further analysis. Cuvier's and Sowerby's clicks occurred at all stations throughout the monitoring period. There was a significant effect of month and station (latitude) on the mean daily number of click detections for both species. Cuvier's clicks were more abundant at lower latitudes while Sowerby's were greater at higher latitudes, particularly in the spring, suggesting a spatial segregation between species, possibly driven by prey preference. Cuvier's occurrence increased in late autumn 2015 off northwest Porcupine Bank, a region of higher relative occurrence for each species. Seismic airgun shots, with daily sound exposure levels as high as 175 dB re 1 μPa2·s, did not appear to impact the mean daily number of Cuvier's or Sowerby's beaked whale click detections. This work provides insight into the significance of Irish waters for beaked whales and highlights the importance of using acoustics for beaked whale monitoring.

Deep Multimodal Distance Metric Learning Using Click Constraints for Image Ranking.

PubMed

Yu, Jun; Yang, Xiaokang; Gao, Fei; Tao, Dacheng

2017-12-01

How do we retrieve images accurately? Also, how do we rank a group of images precisely and efficiently for specific queries? These problems are critical for researchers and engineers to generate a novel image searching engine. First, it is important to obtain an appropriate description that effectively represent the images. In this paper, multimodal features are considered for describing images. The images unique properties are reflected by visual features, which are correlated to each other. However, semantic gaps always exist between images visual features and semantics. Therefore, we utilize click feature to reduce the semantic gap. The second key issue is learning an appropriate distance metric to combine these multimodal features. This paper develops a novel deep multimodal distance metric learning (Deep-MDML) method. A structured ranking model is adopted to utilize both visual and click features in distance metric learning (DML). Specifically, images and their related ranking results are first collected to form the training set. Multimodal features, including click and visual features, are collected with these images. Next, a group of autoencoders is applied to obtain initially a distance metric in different visual spaces, and an MDML method is used to assign optimal weights for different modalities. Next, we conduct alternating optimization to train the ranking model, which is used for the ranking of new queries with click features. Compared with existing image ranking methods, the proposed method adopts a new ranking model to use multimodal features, including click features and visual features in DML. We operated experiments to analyze the proposed Deep-MDML in two benchmark data sets, and the results validate the effects of the method.

Auditory-Nerve Responses to Clicks at Low Levels, and the Initial Peak at High Levels, are Suppressed at Opposite Bias-Tone Phases

NASA Astrophysics Data System (ADS)

Nam, Hui; Guinan, John J.

2011-11-01

Apical auditory nerve (AN) fibers show two click-response regions that are both strongly inhibited by medial olivocochlear (MOC) efferents: (1) ringing responses from low- level (LL) clicks that are thought to be enhanced by a "cochlear amplifier," and (2) AN initial peak (ANIPr) responses from moderate-to-high level (˜70-100 dB pSPL) rarefaction clicks. Since MOC fibers synapse and act on outer hair cells (OHCs), the MOC inhibition of these responses indicates that OHC processes are heavily involved in the production of both LL and ANIPr responses. Using AN recordings in anesthetized cats, we explored the role of OHC stereocilia position in the production of these click-response regions by presenting rarefaction clicks at different phases of 50 Hz, 70-110 dB SPL bias tones. Bias effects on LL responses followed the traditional biasing pattern of twice-a-bias-tone-cycle suppression with more suppression at one phase than the other. This suppression is attributable to the bias tone moving the OHC stereocilia toward low-slope, saturation regions of the mechano-electric transduction function with the rest position being closer to one saturation region. A somewhat similar pattern was found for ANIPr responses except that the bias phases of the largest suppressions were different in ANIPr versus LL responses, usually by ˜180 degrees. The data are consistent with the LL and ANIPr responses both being due to active processes in OHCs that are controlled by OHC stereocilia position. The different phases of the LL and ANIPr suppressions indicate that different mechanisms, and perhaps different vibration patterns in the organ of Corti, are involved in the production of LL and ANIPr responses.

Evaluation of the May 2005 Click It or Ticket mobilization to increase seat belt use

DOT National Transportation Integrated Search

2007-05-01

Click It or Ticket (CIOT) is an intense, short-duration, seat belt publicity and enforcement program. The CIOT May 2005 Mobilization involved approximately $33 million of purchased media. Law enforcement agencies across the Nation reported issuing mo...

Sensory biology: echolocation from click to call, mouth to wing.

PubMed

Fenton, M Brock; Ratcliffe, John M

2014-12-15

Echolocators use echoes of sounds they produce, clicks or calls, to detect objects. Usually, these signals originate from the head. New work reveals that three species of bats use their wings to generate echolocation signals. Copyright © 2014 Elsevier Ltd. All rights reserved.

78 FR 52607 - Unified Registration System

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-23

...'' and click ``Search.'' Next, click ``Open Docket Folder'' in the ``Actions'' column. Finally, in the... comments received are posted without change to http://www.regulations.gov . Anyone is able to search the... License CFR Code of Federal Regulations CMV Commercial Motor Vehicle CR Compliance Review CSA Compliance...

78 FR 24069 - Safety Zone; 12th Annual Saltwater Classic; Port Canaveral Harbor; Port Canaveral, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-24

... ``SEARCH'' box and click ``SEARCH.'' Click on Open Docket Folder on the line associated with this..., 2013, the Cox Events Group and K92.3-FM will host a fishing tournament offshore of Port Canaveral, FL...

Facebook Advertisements for Inexpensive Participant Recruitment Among Women in Early Pregnancy

PubMed Central

Arcia, Adriana

2014-01-01

Facebook advertisements were utilized to recruit nulliparous women in the first 20 weeks of pregnancy for an online survey about their childbirth preferences. A campaign of ads was targeted to women, aged 18-44, residing in the United States. The ads were viewed 10,577,381 times by 7,248,985 unique Facebook users over 18 weeks in 2011. The ad campaign yielded 6,094 clicks by 5,963 unique users at a mean cost of $0.63 per click and a unique click-through rate of 0.08%. Of those who clicked through to the study site, eighteen percent (18%, n = 1,075) consented to participate. The participant pool was reduced to 344 women after application of strict eligibility criteria. Participants represented 43 states and the District of Columbia, their mean age was 20.9 years (Mdn 19.0, SD 4.0), and their mean weeks’ gestation was 11.5 (SD 5.8). The campaign cost was $3,821.81 or $11.11 per eligible participant. PMID:24082026

The effects of click and tone-burst stimulus parameters on the vestibular evoked myogenic potential (VEMP).

PubMed

Akin, Faith Wurm; Murnane, Owen D; Proffitt, Tina M

2003-11-01

Vestibular evoked myogenic potentials (VEMP) are short latency electromyograms (EMG) evoked by high-level acoustic stimuli and recorded from surface electrodes over the tonically contracted sternocleidomastoid (SCM) muscle and are presumed to originate in the saccule. The present experiments examined the effects of click and tone-burst level and stimulus frequency on the latency, amplitude, and threshold of the VEMP in subjects with normal hearing sensitivity and no history of vestibular disease. VEMPs were recorded in all subjects using 100 dB nHL click stimuli. Most subjects had VEMPs present at 500, 750, and 1000 Hz, and few subjects had VEMPs present at 2000 Hz. The response amplitude of the VEMP increased with click and tone-burst level, whereas VEMP latency was not influenced by the stimulus level. The largest tone-burst-evoked VEMPs and lowest thresholds were obtained at 500 and 750 Hz. VEMP latency was independent of stimulus frequency when tone-burst duration was held constant.

Click-electron microscopy for imaging metabolically tagged non-protein biomolecules

PubMed Central

Ngo, John T.; Adams, Stephen R.; Deerinck, Thomas J.; Boassa, Daniela; Rodriguez-Rivera, Frances; Palida, Sakina F.; Bertozzi, Carolyn R.; Ellisman, Mark H.; Tsien, Roger Y.

2016-01-01

Electron microscopy (EM) has long been the main technique to image cell structures with nanometer resolution, but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. Here we introduce “Click-EM,” a labeling technique for correlative light microscopy and EM imaging of non-protein biomolecules. In this approach, metabolic labeling substrates containing bioorthogonal functional groups are provided to cells for incorporation into biopolymers by endogenous biosynthetic machinery. The unique chemical functionality of these analogs is exploited for selective attachment of singlet oxygen-generating fluorescent dyes via bioorthogonal “click chemistry” ligations. Illumination of dye-labeled structures generates singlet oxygen to locally catalyze the polymerization of diaminobenzidine into an osmiophilic reaction product that is readily imaged by EM. We describe the application of Click-EM in imaging metabolically tagged DNA, RNA, and lipids in cultured cells and neurons, and highlight its use in tracking peptidoglycan synthesis in the Gram-positive bacterium Listeria monocytogenes. PMID:27110681

Male sperm whale acoustic behavior observed from multipaths at a single hydrophone

NASA Astrophysics Data System (ADS)

Laplanche, Christophe; Adam, Olivier; Lopatka, Maciej; Motsch, Jean-François

2005-10-01

Sperm whales generate transient sounds (clicks) when foraging. These clicks have been described as echolocation sounds, a result of having measured the source level and the directionality of these signals and having extrapolated results from biosonar tests made on some small odontocetes. The authors propose a passive acoustic technique requiring only one hydrophone to investigate the acoustic behavior of free-ranging sperm whales. They estimate whale pitch angles from the multipath distribution of click energy. They emphasize the close bond between the sperm whale's physical and acoustic activity, leading to the hypothesis that sperm whales might, like some small odontocetes, control click level and rhythm. An echolocation model estimating the range of the sperm whale's targets from the interclick interval is computed and tested during different stages of the whale's dive. Such a hypothesis on the echolocation process would indicate that sperm whales echolocate their prey layer when initiating their dives and follow a methodic technique when foraging.

Visual Influences on Perception of Speech and Nonspeech Vocal-Tract Events

PubMed Central

Brancazio, Lawrence; Best, Catherine T.; Fowler, Carol A.

2009-01-01

We report four experiments designed to determine whether visual information affects judgments of acoustically-specified nonspeech events as well as speech events (the “McGurk effect”). Previous findings have shown only weak McGurk effects for nonspeech stimuli, whereas strong effects are found for consonants. We used click sounds that serve as consonants in some African languages, but that are perceived as nonspeech by American English listeners. We found a significant McGurk effect for clicks presented in isolation that was much smaller than that found for stop-consonant-vowel syllables. In subsequent experiments, we found strong McGurk effects, comparable to those found for English syllables, for click-vowel syllables, and weak effects, comparable to those found for isolated clicks, for excised release bursts of stop consonants presented in isolation. We interpret these findings as evidence that the potential contributions of speech-specific processes on the McGurk effect are limited, and discuss the results in relation to current explanations for the McGurk effect. PMID:16922061

Preparation of Lanthanide-Polymer Composite Material via Click Chemistry.

PubMed

Chen, Bin; Wen, Guian; Wu, Jiajie; Feng, Jiachun

2015-10-01

Covalently attaching lanthanide complexes to the polymer backbone can effectively reduce the clustering of lanthanides and thus become an important strategy to fully unleash their potential. In this Communication, a metal-free click reaction is used for the first time to link a lanthanide complex to the polymer matrix. A diene-bearing copolymer with anthracenylmethyl methacrylate as a monomer and a dienophile-bearing lanthanide complex with 5-maleimido-1,10-phenanthroline as the second ligand are synthesized and coupled together through a Diels-Alder cycloaddition (DA). A comparative investigation demonstrates that the composite material prepared by DA click reaction shows the highest quantum yields in the same lanthanide concentration as compared to materials prepared by widely used "directly doping" and "in situ coordinating lanthanide ions with macromolecular ligand" approaches. This work suggests that the "metal-free" DA click reaction can be a promising tool in the synthesis of high efficient lanthanide functionalized polymeric materials. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

pH-responsive supramolecular self-assembly of well-defined zwitterionic ABC miktoarm star terpolymers.

PubMed

Liu, Hao; Li, Changhua; Liu, Hewen; Liu, Shiyong

2009-04-21

We report the first example of the synthesis and pH-responsive supramolecular self-assembly of double hydrophilic ABC miktoarm star terpolymers. Well-defined ABC miktoarm star terpolymers consisting of poly(ethylene glycol), poly(tert-butyl methacrylate), and poly(2-(diethylamino)ethyl methacrylate) arms [PEG(-b-PtBMA)-b-PDEA] were synthesized via the combination of consecutive click reactions and atom transfer radical polymerization (ATRP), starting from a trifunctional core molecule, 1-azido-3-chloro-2-propanol (ACP). The click reaction of monoalkynyl-terminated PEG with an excess of ACP afforded difunctional PEG bearing a chlorine and a secondary hydroxyl moiety at the chain end, PEG113(-Cl)-OH (1). After azidation with NaN3, PEG-based macroinitiator PEG113(-N3)-Br (3) was prepared by the esterification of PEG113(-N3)-OH (2) with 2-bromoisobutyryl bromide and then employed in the ATRP of tert-butyl methacrylate (tBMA). The obtained PEG(-N3)-b-PtBMA copolymers (4) possessed an azido moiety at the diblock junction point. The preparation of PEG(-b-PtBMA)-b-PDEA miktoarm star terpolymers was then achieved via the click reaction of 4 with an excess of monoalkynyl-terminated PDEA. The obtained miktoarm star terpolymers were successfully converted into PEG(-b-PMAA)-b-PDEA, where PMAA is poly(methacrylic acid). In aqueous solution, PEG(-b-PMAA)-b-PDEA zwitterionic ABC miktoarm star terpolymers can self-assemble into three types of micellar aggregates by simply adjusting solution pH at room temperature. Above pH 8, PDEA-core micelles stabilized by PEG/ionized PMAA hybrid coronas were formed due to the insolubility of PDEA block. In the range of pH 5-7, micelles possessing polyion complex cores formed as a result of charge compensation between partially ionized PMAA and partially protonated PDEA sequences. At pH<4, hydrogen bonding interactions between fully protonated PMAA and PEG led to the formation of another type of micellar aggregates possessing hydrogen-bonded complex cores stabilized by protonated PDEA coronas. The fully reversible pH-responsive formation of three types of aggregates were characterized by 1H NMR, dynamic and static laser light scattering (LLS), and transmission electron microscopy (TEM).

Low-Cost Avoidance Behaviors are Resistant to Fear Extinction in Humans

PubMed Central

Vervliet, Bram; Indekeu, Ellen

2015-01-01

Elevated levels of fear and avoidance are core symptoms across the anxiety disorders. It has long been known that fear serves to motivate avoidance. Consequently, fear extinction has been the primary focus in pre-clinical anxiety research for decades, under the implicit assumption that removing the motivator of avoidance (fear) would automatically mitigate the avoidance behaviors as well. Although this assumption has intuitive appeal, it has received little scientific scrutiny. The scarce evidence from animal studies is mixed, while the assumption remains untested in humans. The current study applied an avoidance conditioning protocol in humans to investigate the effects of fear extinction on the persistence of low-cost avoidance. Online danger-safety ratings and skin conductance responses documented the dynamics of conditioned fear across avoidance and extinction phases. Anxiety- and avoidance-related questionnaires explored individual differences in rates of avoidance. Participants first learned to click a button during a predictive danger signal, in order to cancel an upcoming aversive electrical shock (avoidance conditioning). Next, fear extinction was induced by presenting the signal in the absence of shocks while button-clicks were prevented (by removing the button in Experiment 1, or by instructing not to click the button in Experiment 2). Most importantly, post-extinction availability of the button caused a significant return of avoidant button-clicks. In addition, trait-anxiety levels correlated positively with rates of avoidance during a predictive safety signal, and with the rate of pre- to post-extinction decrease during this signal. Fear measures gradually decreased during avoidance conditioning, as participants learned that button-clicks effectively canceled the shock. Preventing button-clicks elicited a sharp increase in fear, which subsequently extinguished. Fear remained low during avoidance testing, but danger-safety ratings increased again when button-clicks were subsequently prevented. Together, these results show that low-cost avoidance behaviors can persist following fear extinction and induce increased threat appraisal. On the other hand, fear extinction did reduce augmented rates of unnecessary avoidance during safety in trait-anxious individuals, and instruction-based response prevention was more effective than removal of response cues. More research is needed to characterize the conditions under which fear extinction might mitigate avoidance. PMID:26733837

Chemical Modification of Cyclodextrin and Amylose by Click Reaction and Its Application to the Synthesis of Poly-alkylamine-Modified Antibacterial Sugars.

PubMed

Yamamura, Hatsuo

2017-01-01

Cyclodextrin (CD) can be chemically modified into desired and sophisticated functional molecules. However, poly-modification often produces complicated mixtures, resulting in a low yield of the desired product. As the most promising procedure to solve such problems and to achieve poly-modification of the CD molecule, we present here the Huisgen 1,3-dipolar cycloaddition, known as a click reaction. This review will describe the results of our microwave-assisted click reaction for the poly-modification of CD and amylose molecules, and its application to the study of synthetic membrane active antibacterial derivatives.

Functionalization of chitosan by click chemistry

NASA Astrophysics Data System (ADS)

Cheaburu-Yilmaz, Catalina Natalia; Karavana, Sinem Yaprak; Yilmaz, Onur

2017-12-01

Chitosan modification represents a challenge nowadays. The variety of compounds which can be obtained with various architectures and different functionalities made it attractive to be used in fields like pharmacy and material science. Presents study deals with the chemical modification of chitosan by using click chemistry technique. The study adopted the approach of clicking azidated chitosan with a synthesized alkyne terminated polymer i.e. poly N isopropylacrylamide with thermoresponsive properties. Structures were confirmed by the FT-IR and HNMR spectra. Thermal characterization was performed showing different thermal behaviour with the chemical modification. The final synthesized graft copolymer can play important role within pharmaceutical formulations carrying drugs for topical or oral treatments.

Products and Services Available from the Southern California Earthquake Data Center (SCEDC) and the Southern California Seismic Network (SCSN)

NASA Astrophysics Data System (ADS)

Chen, S. E.; Yu, E.; Bhaskaran, A.; Chowdhury, F. R.; Meisenhelter, S.; Hutton, K.; Given, D.; Hauksson, E.; Clayton, R. W.

2011-12-01

Currently, the SCEDC archives continuous and triggered data from nearly 8400 data channels from 425 SCSN recorded stations, processing and archiving an average of 6.4 TB of continuous waveforms and 12,000 earthquakes each year. The SCEDC provides public access to these earthquake parametric and waveform data through its website www.data.scec.org and through client applications such as STP and DHI. This poster will describe the most significant developments at the SCEDC during 2011. New website design: ? The SCEDC has revamped its website. The changes make it easier for users to search the archive, discover updates and new content. These changes also improve our ability to manage and update the site. New data holdings: ? Post processing on El Mayor Cucapah 7.2 sequence continues. To date there have been 11847 events reviewed. Updates are available in the earthquake catalog immediately. ? A double difference catalog (Hauksson et. al 2011) spanning 1981 to 6/30/11 will be available for download at www.data.scec.org and available via STP. ? A focal mechanism catalog determined by Yang et al. 2011 is available for distribution at www.data.scec.org. ? Waveforms from Southern California NetQuake stations are now being stored in the SCEDC archive and available via STP as event associated waveforms. Amplitudes from these stations are also being stored in the archive and used by ShakeMap. ? As part of a NASA/AIST project in collaboration with JPL and SIO, the SCEDC will receive real time 1 sps streams of GPS displacement solutions from the California Real Time Network (http://sopac.ucsd.edu/projects/realtime; Genrich and Bock, 2006, J. Geophys. Res.). These channels will be archived at the SCEDC as miniSEED waveforms, which then can be distributed to the user community via applications such as STP. Improvements in the user tool STP: ? STP sac output now includes picks from the SCSN. New archival methods: ? The SCEDC is exploring the feasibility of archiving and distributing waveform data using cloud computing such as Google Apps. A month of continuous data from the SCEDC archive will be stored in Google Apps and a client developed to access it in a manner similar to STP. The data is stored in miniseed format with gzip compression. Time gaps between time series were padded with null values, which substantially increases search efficiency by make the records uniform in length.

Analyzing the first years of the click it or ticket mobilizations.

DOT National Transportation Integrated Search

2010-01-01

The Click It or Ticket seat belt enforcement programs conducted between 2000 and 2006 were an important factor in increasing seat belt use nationwide and in virtually all States. This was the case for observed belt use, belt use in fatalities, and se...

Understanding Mircrobial Sensing in Inflammatory Bowel Disease Using Click Chemistry

DTIC Science & Technology

2016-10-01

limitation, we have developed an expanded metabolic labeling approach that chemically tags lipopolysaccharide, capsular polysaccharide , and peptidoglycan...click-chemistry, bacterial cell wall, bacterial outer membrane, peptidoglycan, lipopolysaccharide, endotoxin, capsular polysaccharide , inflammatory...bacterial outer membrane, peptidoglycan, lipopolysaccharide, endotoxin, capsular polysaccharide , inflammatory bowel disease, microbiome, microbiota

Thermodynamics and kinetics of DNA nanotube polymerization from single-filament measurements† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c3sc53331j Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file.

PubMed Central

Hariadi, Rizal F.; Yurke, Bernard

2015-01-01

DNA nanotubes provide a programmable architecture for molecular self-assembly and can serve as model systems for one-dimensional biomolecular assemblies. While a variety of DNA nanotubes have been synthesized and employed as models for natural biopolymers, an extensive investigation of DNA nanotube kinetics and thermodynamics has been lacking. Using total internal reflection microscopy, DNA nanotube polymerization was monitored in real time at the single filament level over a wide range of free monomer concentrations and temperatures. The measured polymerization rates were subjected to a global nonlinear fit based on polymerization theory in order to simultaneously extract kinetic and thermodynamic parameters. For the DNA nanotubes used in this study, the association rate constant is (5.99 ± 0.15) × 105 M–1 s–1, the enthalpy is 87.9 ± 2.0 kcal mol–1, and the entropy is 0.252 ± 0.006 kcal mol–1 K–1. The qualitative and quantitative similarities between the kinetics of DNA nanotubes, actin filaments, and microtubules polymerization highlight the prospect of building complex dynamic systems from DNA molecules inspired by biological architecture. PMID:29308139

Neural coding of repetitive clicks in the medial geniculate body of cat.

PubMed

Rouiller, E; de Ribaupierre, Y; Toros-Morel, A; de Ribaupierre, F

1981-09-01

The activity of 418 medial geniculate body (MGB) units was studied in response to repetitive acoustic pulses in 35 nitrous oxide anaesthetized cats. The proportion of MGB neurons insensitive to repetitive clicks was close to 30%. On the basis of their pattern of discharge, the responsive units were divided into three categories. The majority of them (71%), classified as "lockers', showed discharges precisely time-locked to the individual clicks of the train. A few units (8%), called "groupers', had discharges loosely synchronized to low-rate repetitive clicks. When the spikes were not synchronized, the cell had transient or sustained responses for a limited frequency range and was classified as a "special responder' (21%). Responses of "lockers' were time-locked up to a limiting rate, which varied between 10 and 800 Hz; half of the "lockers' had a limiting rate of locking equal to or higher than 100 Hz. The degree of entrainment, defined as the probability that each click evokes at least one spike, regularly decreases for increasing rates; on the other hand, the precision of locking increasing increases with frequency. The time jitter observed at 100 Hz might be as small as 0.2 ms and was 1.2 ms on average. The population of "lockers' can mark with precision the transients of complex sounds and has response properties still compatible with a temporal coding of the fundamental frequency of most animal vocalizations.

Injectable dextran hydrogels fabricated by metal-free click chemistry for cartilage tissue engineering.

PubMed

Wang, Xiaoyu; Li, Zihan; Shi, Ting; Zhao, Peng; An, Kangkang; Lin, Chao; Liu, Hongwei

2017-04-01

Injectable dextran-based hydrogels were prepared for the first time by bioorthogonal click chemistry for cartilage tissue engineering. Click-crosslinked injectable hydrogels based on cyto-compatible dextran (Mw=10kDa) were successfully fabricated under physiological conditions by metal-free alkyne-azide cycloaddition (click) reaction between azadibenzocyclooctyne-modified dextran (Dex-ADIBO) and azide-modified dextran (Dex-N 3 ). Gelation time of these dextran hydrogels could be regulated in the range of approximately 1.1 to 10.2min, depending on the polymer concentrations (5% or 10%) and ADIBO substitution degree (DS, 5 or 10) of Dex-ADIBO. Rheological analysis indicated that the dextran hydrogels were elastic and had storage moduli from 2.1 to 6.0kPa with increasing DS of ADIBO from 5 to 10. The in vitro tests revealed that the dextran hydrogel crosslinked from Dex-ADIBO DS 10 and Dex-N 3 DS 10 at a polymer concentration of 10% could support high viability of individual rabbit chondrocytes and the chondrocyte spheroids encapsulated in the hydrogel over 21days. Individual chondrocytes and chondrocyte spheroids in the hydrogel could produce cartilage matrices such as collagen and glycosaminoglycans. However, the chondrocyte spheroids produced a higher content of matrices than individual chondrocytes. This study indicates that metal-free click chemistry is effective to produce injectable dextran hydrogels for cartilage tissue engineering. Copyright © 2016 Elsevier B.V. All rights reserved.

One-pot reaction for the preparation of biofunctionalized self-assembled monolayers on gold surfaces

NASA Astrophysics Data System (ADS)

Raigoza, Annette F.; Fies, Whitney; Lim, Amber; Onyirioha, Kristeen; Webb, Lauren J.

2017-02-01

The Huisgen cycloaddition reaction (;click; chemistry) has been used extensively to functionalize surfaces with macromolecules in a straightforward manner. We have previously developed a procedure using the copper(I)-catalyzed click reaction to tether synthetic α-helical peptides carrying two alkyne groups to a well-ordered azide-terminated alkanethiol self-assembled monolayer (SAM) on a Au(111) surface. While convenient, click-based strategies potentially pose significant problems from reagents, solvents, and reaction temperatures that may irreversibly damage some molecules or substrates. Tuning click chemistry conditions would allow individual optimization of reaction conditions for a wide variety of biomolecules and substrate materials. Here, we explore the utility of simultaneous SAM formation and peptide-attachment chemistry in a one-pot reaction. We demonstrate that a formerly multistep reaction can be successfully carried out concurrently by mixing azide-terminated alkanethiols, CuCl, and a propargylglycine-containing peptide over a bare gold surface in ethanol and reacting at 70 °C. X-ray photoelectron spectroscopy (XPS), surface infrared spectroscopy, surface circular dichroic (CD) spectroscopy, and scanning tunneling microscopy (STM) were used to determine that this one-pot reaction strategy resulted in a high density of surface-bound α-helices without aggregation. This work demonstrates the simplicity and versatility of a SAM-plus-click chemistry strategy for functionalizing Au surfaces with structured biomolecules.

Echolocation clicks of free-ranging Cuvier's beaked whales (Ziphius cavirostris)

NASA Astrophysics Data System (ADS)

Zimmer, Walter M. X.; Johnson, Mark P.; Madsen, Peter T.; Tyack, Peter L.

2005-06-01

Strandings of beaked whales of the genera Ziphius and Mesoplodon have been reported to occur in conjunction with naval sonar use. Detection of the sounds from these elusive whales could reduce the risk of exposure, but descriptions of their vocalizations are at best incomplete. This paper reports quantitative characteristics of clicks from deep-diving Cuvier's beaked whales (Ziphius cavirostris) using a unique data set. Two whales in the Ligurian Sea were simultaneously tagged with sound and orientation recording tags, and the dive tracks were reconstructed allowing for derivation of the range and relative aspect between the clicking whales. At depth, the whales produced trains of regular echolocation clicks with mean interclick intervals of 0.43 s (+/-0.09) and 0.40 s (+/-0.07). The clicks are frequency modulated pulses with durations of ~200 μs and center frequencies around 42 kHz, -10 dB bandwidths of 22 kHz, and Q3 dB of 4. The sound beam is narrow with an estimated directionality index of more than 25 dB, source levels up to 214 dBpp re: 1 μPa at 1 m, and energy flux density of 164 dB re: 1 μPa2 s. As the spectral and temporal properties are different from those of nonziphiid odontocetes the potential for passive detection is enhanced. .

One-step fabrication of PEGylated fluorescent nanodiamonds through the thiol-ene click reaction and their potential for biological imaging

NASA Astrophysics Data System (ADS)

Huang, Hongye; Liu, Meiying; Tuo, Xun; Chen, Junyu; Mao, Liucheng; Wen, Yuanqing; Tian, Jianwen; Zhou, Naigen; Zhang, Xiaoyong; Wei, Yen

2018-05-01

Over the past years, fluorescent carbon nanoparticles have got growing interest for biological imaging. Fluorescent nanodiamonds (FNDs) are novel fluorescent carbon nanoparticles with multitudinous useful properties, including remarkable fluorescence properties, extremely low toxicity and high refractive index. However, facile preparation of FNDs with designable properties and functions from non-fluorescent detonation nanodiamonds (DNDs) has demonstrated to be challengeable. In this work, we reported for the first time that preparation of Polyethylene glycol (PEG) functionalized FNDs through a one-step thiol-ene click reaction using thiol containing PEG (PEG-SH) as the coating agent. Based on the characterization results, we demonstrated that PEG-SH could be efficiently introduced on DNDs to obtain FNDs through the thiol-ene click chemistry. The resultant FND-PEG composites showed high water dispersibility, strong fluorescence and low cytotoxicity. Moreover, FND-PEG composites could be internalized by cells and displayed good cell dyeing performance. All of these features implied that FND-PEG composites are of great potential for biological imaging. Taken together, a facile one-step strategy based on the one-step thiol-ene click reaction has been developed for efficient preparation of FND-PEG composites from non-fluorescent DNDs. The strategy should be also useful for fabrication of many other functional FNDs via using different thiol containing compounds for the universality of thiol-ene click reaction.

Comparison Study of Two Differently Clicked 18F-Folates—Lipophilicity Plays a Key Role

PubMed Central

Kettenbach, Kathrin; Reffert, Laura M.; Schieferstein, Hanno; Pektor, Stefanie; Eckert, Raphael; Miederer, Matthias; Rösch, Frank

2018-01-01

Within the last decade, several folate-based radiopharmaceuticals for Single Photon Emission Computed Tomography (SPECT) and Positron Emission Tomography (PET) have been evaluated; however, there is still a lack of suitable 18F-folates for clinical PET imaging. Herein, we report the synthesis and evaluation of two novel 18F-folates employing strain-promoted and copper-catalyzed click chemistry. Furthermore, the influence of both click-methods on lipophilicity and pharmacokinetics of the 18F-folates was investigated. 18F-Ala-folate and 18F-DBCO-folate were both stable in human serum albumin. In vitro studies proved their high affinity to the folate receptor (FR). The lipophilic character of the strain-promoted clicked 18F-DBCO-folate (logD = 0.6) contributed to a higher non-specific binding in cell internalization studies. In the following in vivo PET imaging studies, FR-positive tumors could not be visualized in a maximum intensity projection images. Compared with 18F-DBCO-folate, 18F-Ala-folate (logD = −1.4), synthesized by the copper-catalyzed click reaction, exhibited reduced lipophilicity, and as a result an improved in vivo performance and a clear-cut visualization of FR-positive tumors. In view of high radiochemical yield, radiochemical purity and favorable pharmacokinetics, 18F-Ala-folate is expected to be a promising candidate for FR-PET imaging. PMID:29562610

Design of a thermally controlled sequence of triazolinedione-based click and transclick reactions† †Electronic supplementary information (ESI) available: Additional figures, experimental details, synthesis and analysis of all the model compounds and polymers, computational methods and relevant theoretical data. See DOI: 10.1039/c7sc00119c Click here for additional data file.

PubMed Central

Houck, Hannes A.; De Bruycker, Kevin; Billiet, Stijn; Dhanis, Bastiaan; Goossens, Hannelore; Catak, Saron; Van Speybroeck, Veronique

2017-01-01

The reaction of triazolinediones (TADs) and indoles is of particular interest for polymer chemistry applications, as it is a very fast and irreversible additive-free process at room temperature, but can be turned into a dynamic covalent bond forming process at elevated temperatures, giving a reliable bond exchange or ‘transclick’ reaction. In this paper, we report an in-depth study aimed at controlling the TAD–indole reversible click reactions through rational design of modified indole reaction partners. This has resulted in the identification of a novel class of easily accessible indole derivatives that give dynamic TAD-adduct formation at significantly lower temperatures. We further demonstrate that these new substrates can be used to design a directed cascade of click reactions of a functionalized TAD moiety from an initial indole reaction partner to a second indole, and finally to an irreversible reaction partner. This controlled sequence of click and transclick reactions of a single TAD reagent between three different substrates has been demonstrated both on small molecule and macromolecular level, and the factors that control the reversibility profiles have been rationalized and guided by mechanistic considerations supported by theoretical calculations. PMID:28507685

The CUORE slow monitoring systems

NASA Astrophysics Data System (ADS)

Gladstone, L.; Biare, D.; Cappelli, L.; Cushman, J. S.; Del Corso, F.; Fujikawa, B. K.; Hickerson, K. P.; Moggi, N.; Pagliarone, C. E.; Schmidt, B.; Wagaarachchi, S. L.; Welliver, B.; Winslow, L. A.

2017-09-01

CUORE is a cryogenic experiment searching primarily for neutrinoless double decay in 130Te. It will begin data-taking operations in 2016. To monitor the cryostat and detector during commissioning and data taking, we have designed and developed Slow Monitoring systems. In addition to real-time systems using LabVIEW, we have an alarm, analysis, and archiving website that uses MongoDB, AngularJS, and Bootstrap software. These modern, state of the art software packages make the monitoring system transparent, easily maintainable, and accessible on many platforms including mobile devices.

The cataclysmic variable AE Aquarii: orbital variability in V band

NASA Astrophysics Data System (ADS)

Zamanov, R.; Latev, G.

2017-07-01

We present 62.7 hours observations of the cataclysmic variable AE Aqr in Johnson V band. These are non-published archive electro-photometric data obtained during the time period 1993 to 1999. We construct the orbital variability in V band and obtain a Fourier fit to the double-wave quiescent light curve. The strongest flares in our data set are in phase interval 0.6 - 0.8. The data can be downloaded from http://www.astro.bas.bg/~rz/DATA/AEAqr.elphot.dat.

78 FR 26575 - Gross Combination Weight Rating; Definition

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-07

... ``Rules,'' insert ``FMCSA-2012-0156'' in the ``Keyword'' box, and click ``Search.'' When the new screen... side of the screen. Then, in the ``Keyword'' box insert ``FMCSA-2012-0156'' and click ``Search.'' Next... you provide. Anyone is able to search the electronic form of all comments received into any of our...

GEONETCast Americas - Architecture

Science.gov Websites

FengYunCast covers Asia. See the following picture for a global view of the planned GEONETCast satellite participants. (Click drawing to enlarge) Map of world showing satellite size required to receive GEONETCast Americas according to location Satellite dish size required, according to location. (Click drawing to

"Just Clicks": An Interpretive Phenomenological Analysis of Professional Dancers' Experience of Flow

ERIC Educational Resources Information Center

Hefferon, Kate M.; Ollis, Stewart

2006-01-01

The subjective experience of flow in professional dancers was analyzed using interpretive phenomenological analysis (IPA). Flow is believed to be a psychological state in which the mind and body "just click", creating optimal performance. Unfortunately, sport and performance research have severely neglected reviewing the flow experience in…

Improvements to Passive Acoustic Tracking Methods for Marine Mammal Monitoring

DTIC Science & Technology

2015-09-30

applicable for highly directional sources). The MTOA+RL method was applied to a dolphin click sequence from a single hydrophone dataset. Using arrival...arrival and 3 multipath arrivals for a dolphin click recorded on a single seafloor-mounted hydrophone. The hydrophone [described in Fedenczuk et al

Spatially Resolved Imaging and Spectroscopy of Candidate Dual Active Galactic Nuclei

NASA Astrophysics Data System (ADS)

McGurk, R. C.; Max, C. E.; Medling, A. M.; Shields, G. A.; Comerford, J. M.

2015-09-01

When galaxies merge, both central supermassive black holes are immersed in a dense and chaotic environment. If there is sufficient gas in the nuclear regions, one expects to see close pairs of active galactic nuclei (AGNs), or dual AGNs, in a fraction of galaxy mergers. However, finding them remains a challenge. The presence of double-peaked [O iii] emission lines has been proposed as a technique to select dual AGNs efficiently. We studied a sample of double-peaked narrow [O iii] emitting AGNs from Sloan Digital Sky Survey (SDSS) DR7. By obtaining new and archival high spatial resolution images taken with the Keck II Laser Guide Star Adaptive Optics system and the near-infrared camera NIRC2, we show that 30% of 140 double-peaked [O iii] emission line SDSS AGNs have two spatial components within a 3″ radius. However, spatially resolved spectroscopy or X-ray observations are needed to confirm these galaxy pairs as systems containing two AGNs. We followed up three spatially double candidate dual AGNs with integral field spectroscopy from Keck OSIRIS and 10 candidates with long-slit spectroscopy from the Shane Kast Double Spectrograph at Lick Observatory. We find that the double-peaked emission lines in our sample of 12 candidates are caused by: one dual AGN (SDSS J114642.47+511029.6), one confirmed outflow and four likely outflows, two pairs of star-forming galaxies, one candidate indeterminate due to sky line interference, and three AGNs with spatially coincident double [O iii] peaks, likely due to unresolved complex narrow line kinematics, outflows, binary AGN, or small-scale jets.

Virtual microscopy in a veterinary curriculum.

PubMed

Sims, Michael H; Mendis-Handagama, Chamindrani; Moore, Robert N

2007-01-01

Teaching faculty in the University of Tennessee College of Veterinary Medicine assist students in their professional education by providing a new way of viewing microscopic slides digitally. Faculty who teach classes in which glass slides are used participate in a program called Virtual Microscopy. Glass slides are digitized using a state-of-the-art integrated system, and a personal computer functions as the "microscope." Additionally, distribution of the interactive images is enhanced because they are available to students online. The digital slide offers equivalent quality and resolution to the original glass slide viewed on a microscope and has several additional advantages over microscopes. Students can choose to examine the entire slide at any of several objectives; they are able to access the slides (called WebSlides) from the college's server, using either Internet Explorer or a special browser developed by Bacus Laboratories, Inc.,(a) called the WebSlide browser, which lets the student simultaneously view a low-objective image and one or two high-objective images of the same slide. The student can "move the slide" by clicking and dragging the image to a new location. Easy archiving, annotation of images, and Web conferencing are additional features of the system.

Ultralow protein adsorbing coatings from clickable PEG nanogel solutions: Benefits of attachment under salt-induced phase separation conditions and comparison with PEG/albumin nanogel coatings

PubMed Central

Donahoe, Casey D.; Cohen, Thomas L.; Li, Wenlu; Nguyen, Peter K.; Fortner, John D.; Mitra, Robi D.; Elbert, Donald L.

2013-01-01

Clickable nanogel solutions were synthesized by using the copper catalyzed azide/alkyne cycloaddition (CuAAC) to partially polymerize solutions of azide and alkyne functionalized poly(ethylene glycol) (PEG) monomers. Coatings were fabricated using a second click reaction: a UV thiol-yne attachment of the nanogel solutions to mercaptosilanated glass. Because the CuAAC reaction was effectively halted by the addition of a copper-chelator, we were able to prevent bulk gelation and limit the coating thickness to a single monolayer of nanogels in the absence of the solution reaction. This enabled the inclusion of kosmotropic salts, which caused the PEG to phase-separate and nearly double the nanogel packing density, as confirmed by Quartz Crystal Microbalance with Dissipation (QCM-D). Protein adsorption was analyzed by single molecule counting with total internal reflection fluorescence (TIRF) microscopy and cell adhesion assays. Coatings formed from the phase-separated clickable nanogel solutions attached with salt adsorbed significantly less fibrinogen than other 100% PEG coatings tested, as well as poly-L-lysine-g-PEG (PLL-g-PEG) coatings. However, PEG/albumin nanogel coatings still outperformed the best 100% PEG clickable nanogel coatings. Additional surface crosslinking of the clickable nanogel coating in the presence of copper further reduced levels of fibrinogen adsorption closer to those of PEG/albumin nanogel coatings. However, this step negatively impacted long-term resistance to cell adhesion and dramatically altered the morphology of the coating by atomic force microscopy (AFM). The main benefit of the click strategy is that the partially polymerized solutions are stable almost indefinitely, allowing attachment in the phase-separated state without danger of bulk gelation, and thus, producing the best performing 100% PEG coating that we have studied to date. PMID:23441808

Consistent interactive segmentation of pulmonary ground glass nodules identified in CT studies

NASA Astrophysics Data System (ADS)

Zhang, Li; Fang, Ming; Naidich, David P.; Novak, Carol L.

2004-05-01

Ground glass nodules (GGNs) have proved especially problematic in lung cancer diagnosis, as despite frequently being malignant they characteristically have extremely slow rates of growth. This problem is further magnified by the small size of many of these lesions now being routinely detected following the introduction of multislice CT scanners capable of acquiring contiguous high resolution 1 to 1.25 mm sections throughout the thorax in a single breathhold period. Although segmentation of solid nodules can be used clinically to determine volume doubling times quantitatively, reliable methods for segmentation of pure ground glass nodules have yet to be introduced. Our purpose is to evaluate a newly developed computer-based segmentation method for rapid and reproducible measurements of pure ground glass nodules. 23 pure or mixed ground glass nodules were identified in a total of 8 patients by a radiologist and subsequently segmented by our computer-based method using Markov random field and shape analysis. The computer-based segmentation was initialized by a click point. Methodological consistency was assessed using the overlap ratio between 3 segmentations initialized by 3 different click points for each nodule. The 95% confidence interval on the mean of the overlap ratios proved to be [0.984, 0.998]. The computer-based method failed on two nodules that were difficult to segment even manually either due to especially low contrast or markedly irregular margins. While achieving consistent manual segmentation of ground glass nodules has proven problematic most often due to indistinct boundaries and interobserver variability, our proposed method introduces a powerful new tool for obtaining reproducible quantitative measurements of these lesions. It is our intention to further document the value of this approach with a still larger set of ground glass nodules.

Nanoplasmonics tuned "click chemistry".

PubMed

Tijunelyte, I; Guenin, E; Lidgi-Guigui, N; Colas, F; Ibrahim, J; Toury, T; Lamy de la Chapelle, M

2016-04-07

Nanoplasmonics is a growing field of optical condensed matter science dedicated to optical phenomena at the nanoscale level in metal systems. Extensive research on noble metallic nanoparticles (NPs) has emerged within the last two decades due to their ability to keep the optical energy concentrated in the vicinity of NPs, in particular, the ability to create optical near-field enhancement followed by heat generation. We have exploited these properties in order to induce a localised "click" reaction in the vicinity of gold nanostructures under unfavourable experimental conditions. We demonstrate that this reaction can be controlled by the plasmonic properties of the nanostructures and we propose two physical mechanisms to interpret the observed plasmonic tuning of the "click" chemistry.

NCEP Operational HWRF Forecasting System

Science.gov Websites

NOAA logo - Click to go to the NOAA homepage EMC Hurricane Team NWS logo - Click to go to the NWS © 2018 | NOAA * NWS * NCEP * EMC * Hurricane Project Team DISCLAIMER: THIS INFORMATION IS PROVIDED AS GUIDANCE. IT REQUIRES INTERPRETATION BY HURRICANE SPECIALISTS AND SHOULD NOT BE CONSIDERED AS A FINAL

78 FR 2650 - Safety Zone, Atlantic Intracoastal Waterway; Wrightsville Beach, NC

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-14

... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket Number USCG-2012-1082] RIN... the docket number (USCG-2012-1082) in the ``SEARCH'' box and click ``SEARCH.'' Click on ``Submit a..., go to http://www.regulations.gov , type the docket number (USCG-2012-1082) in the ``SEARCH'' box and...

NCEP Quarterly Newsletter

Science.gov Websites

second (40-level, different physics/PBL) ARW member is added for all domains (except Guam). The two ARW Skip Navigation Links weather.gov NOAA logo - Click to go to the NOAA homepage National Weather Service NWS logo - Click to go to the NWS homepage National Centers for Environmental Prediction

77 FR 28860 - Notice of Proposed Information Collection Requests; Federal Student Aid; Federal Perkins Loan...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-16

... comments on or before July 16, 2012. ADDRESSES: Written comments regarding burden and/or the collection... Collections'' link and by clicking on link number 04850. When you access the information collection, click on... that Federal agencies provide interested parties an early opportunity to comment on information...

To Click or Not to Click: That's the Question

ERIC Educational Resources Information Center

El-Rady, Johnny

2006-01-01

Classes of 100 students or more are not uncommon in higher education. With such large enrollments, it can be difficult for instructors to integrate active learning components to the traditional lecture format typically adopted in such large classes. To help address this problem, Johnny El-Rady discusses his use of an electronic classroom voting…

78 FR 76704 - Qualification of Drivers; Exemption Applications; Vision

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-18

... year (a) by an ophthalmologist or optometrist who attests that the vision in the better eye continues... submitted evidence showing that the vision in the better eye continues to meet the requirement specified at...; FMCSA- 2007-0017 and click the search button. When the new screen appears, click on the blue ``Comment...

Using "click-e-bricks" to make 3D elastomeric structures.

PubMed

Morin, Stephen A; Shevchenko, Yanina; Lessing, Joshua; Kwok, Sen Wai; Shepherd, Robert F; Stokes, Adam A; Whitesides, George M

2014-09-10

Soft, 3D elastomeric structures and composite structures are easy to fabricate using click-e-bricks, and the internal architecture of these structures together with the capabilities built into the bricks themselves provide mechanical, optical, electrical, and fluidic functions. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Brick & Click Libraries: An Academic Library Conference (14th, Maryville, Missouri, November 7, 2014)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Johnson, Carolyn, Ed.

2014-01-01

Eighteen scholarly papers and twelve abstracts comprise the content of the fourteenth annual Brick and Click Libraries Symposium, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship.…

Brick & Click Libraries: An Academic Library Conference Proceedings (16th, Maryville, Missouri, November 4, 2016)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Johnson, Carolyn, Ed.

2016-01-01

Twenty scholarly papers and fifteen abstracts comprise the content of the sixteenth annual Brick and Click Libraries Conference, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship. The…

MMAB Developmental Products

Science.gov Websites

Skip Navigation Links www.nws.noaa.gov NOAA logo - Click to go to the NOAA homepage National Weather Service NWS logo - Click to go to the NWS homepage Environmental Modeling Center Home News Organization Search Search Search by city or zip code. Press enter or select the go button to submit request

Brick and Click Libraries: Proceedings of an Academic Libraries Symposium (7th, Maryville, Missouri, November 2, 2007)

ERIC Educational Resources Information Center

Ury, Connie Jo, Ed.; Baudino, Frank, Ed.; Park, Sarah G., Ed.

2007-01-01

Twenty-three scholarly papers and eleven abstracts reflect the content of the seventh "Brick and Click Libraries Symposium," held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of…

76 FR 30890 - Special Local Regulations; Sabine River, Orange, TX

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-27

.... Department of Transportation, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE...-0194'' in the ``Keyword'' box. Click ``Search'' then click on the balloon shape in the ``Actions...'' column. You may also visit the Docket Management Facility in Room W12-140 on the ground floor of the...

77 FR 47789 - Drawbridge Operation Regulation; Sacramento River, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-10

..., West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590-0001. (4...'' in the ``Keyword'' box. Click ``Search'' then click on the balloon shape in the ``Actions'' column... also visit the Docket Management Facility in Room W12-140 on the ground floor of the Department of...

75 FR 69371 - Safety Zone; Beaufort River/Atlantic Intracoastal Waterway, Beaufort, SC

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-12

... is necessary to protect life and property on the navigable waters of the Beaufort River during the... Management Facility (M-30), U.S. Department of Transportation, West Building Ground Floor, Room W12-140, 1200...-0995'' in the ``Keyword'' box. Click ``Search'' then click on the balloon shape in the ``Actions...

Recognizing Student Emotions Using Brainwaves and Mouse Behavior Data

ERIC Educational Resources Information Center

Azcarraga, Judith; Suarez, Merlin Teodosia

2013-01-01

Brainwaves (EEG signals) and mouse behavior information are shown to be useful in predicting academic emotions, such as confidence, excitement, frustration and interest. Twenty five college students were asked to use the Aplusix math learning software while their brainwaves signals and mouse behavior (number of clicks, duration of each click,…

Ephemeral Relevance and User Activities in a Search Session

ERIC Educational Resources Information Center

Jiang, Jiepu

2016-01-01

We study relevance judgment and user activities in a search session. We focus on ephemeral relevance--a contextual measurement regarding the amount of useful information a searcher acquired from a clicked result at a particular time--and two primary types of search activities--query reformulation and click. The purpose of the study is both…

77 FR 59597 - Notice of Proposed Information Collection Requests; Office of Elementary and Secondary Education...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-28

... DEPARTMENT OF EDUCATION Notice of Proposed Information Collection Requests; Office of Elementary... information collection request may be accessed from http://edicsweb.ed.gov , by selecting the ``Browse Pending Collections'' link and by clicking on link number 04940. When you access the information collection, click on...

Brick & Click Libraries: An Academic Library Symposium (13th, Maryville, Missouri, November 1, 2013)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Johnson, Carolyn, Ed.; Park, Sarag G., Ed.

2013-01-01

Twenty-six scholarly papers and ten abstracts comprise the content of the thirteenth annual Brick and Click Libraries Symposium, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship. The…

Brick & Click Libraries: An Academic Library Conference (15th, Maryville, Missouri, November 6, 2015)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Johnson, Carolyn, Ed.

2015-01-01

Nineteen scholarly papers and seventeen abstracts comprise the content of the fifteenth annual Brick and Click Libraries Conference, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship.…

Click, Clack, Move: Facilitation of the Arts as Transformative Pedagogy

ERIC Educational Resources Information Center

Vettraino, Elinor; Linds, Warren; Goulet, Linda

2013-01-01

This article explores the arts' potential to transform the relationships between students and teachers, so that education becomes an "as if" world, where education is an act of social justice. Interweaving themes from the children's book "Click Clack Moo, Cows that Type" with theories of transformative pedagogy and their own…

2017 Brick & Click: An Academic Conference (17th, Maryville, Missouri, November 3, 2017)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Hart, Kathy, Ed.; Johnson, Carolyn, Ed.

2017-01-01

Eighteen scholarly papers and fifteen abstracts comprise the content of the seventeenth annual Brick and Click Libraries Conference, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship.…

Fluctuations of Broadband Acoustic Signals in Shallow Water

DTIC Science & Technology

2015-09-30

ships, strumming noise at 5 and 20 Hz, etc. In the Fig. 11, chirp signals from R/V Sharp were recorded. Whistles and clicks by dolphin are also observed...Fig 10. Low frequency ambient noise measurements. click chirp unknown Fig 11. Ambient noise measurements up to 9 kHz. Chirp signals and dolphin

Study of the Effect of Grafting Method on Surface Polarity of Tempo-Oxidized Nanocellulose Using Polycaprolactone as the Modifying Compound: Esterification versus Click-Chemistry

PubMed Central

Benkaddour, Abdelhaq; Jradi, Khalil; Robert, Sylvain; Daneault, Claude

2013-01-01

Esterification and click-chemistry were evaluated as surface modification treatments for TEMPO-oxidized nanocelluloses (TONC) using Polycaprolactone-diol (PCL) as modifying compound in order to improve the dispersion of nanofibers in organic media. These two grafting strategies were analyzed and compared. The first consists of grafting directly the PCL onto TONC, and was carried out by esterification between hydroxyl groups of PCL and carboxyl groups of TONC. The second strategy known as click-chemistry is based on the 1,3-dipolar cycloaddition reaction between azides and alkyne terminated moieties to form the triazole ring between PCL and TONC. The grafted samples were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and Thermogravimetry analysis (TGA). Further, the effects of the two treatments on the surface hydrophobization of TONC were investigated by contact angle measurements. The results show that both methods confirm the success of such a modification and the click reaction was significantly more effective than esterification. PMID:28348357

Facebook Advertisements for Inexpensive Participant Recruitment Among Women in Early Pregnancy.

PubMed

Arcia, Adriana

2014-06-01

Facebook advertisements were used to recruit nulliparous women in the first 20 weeks of pregnancy for an online survey about their childbirth preferences. A campaign of ads was targeted to women, aged 18 to 44 years, residing in the United States. The ads were viewed 10,577,381 times by 7,248,985 unique Facebook users over 18 weeks in 2011. The ad campaign yielded 6,094 clicks by 5,963 unique users at a mean cost of $0.63 per click and a unique click-through rate of 0.08%. Of those who clicked through to the study site, 18% (n = 1,075) consented to participate. The participant pool was reduced to 344 women after application of strict eligibility criteria. Participants represented 43 states and the District of Columbia, their mean age was 20.9 years (Mdn = 19.0, SD = 4.0), and their mean weeks' gestation was 11.5 (SD = 5.8). The campaign cost was $3,821.81 or $11.11 per eligible participant. © 2013 Society for Public Health Education.

Acoustic characteristics of biosonar sounds of free-ranging botos (Inia geoffrensis) and tucuxis (Sotalia fluviatilis) in the Negro River, Amazon, Brazil.

PubMed

Yamamoto, Yukiko; Akamatsu, Tomonari; da Silva, Vera M F; Yoshida, Yayoi; Kohshima, Shiro

2015-08-01

Odontoceti emit broadband high-frequency clicks on echolocation for orientation or prey detection. In the Amazon Basin, two odontoceti species, boto (Amazon River dolphin, Inia geoffrensis) and tucuxi (Sotalia fluviatilis), live sympatrically. The acoustic characteristics of the echolocation clicks of free-ranging botos and tucuxis were measured with a hydrophone array consisting of a full-band and an acoustic event recorder (A-tag). The clicks of the two species were short-duration broadband signals. The apparent source level was 201 dB 1 μPa peak-to-peak at 1 m in the botos and 181 dB 1 μPa peak-to-peak at 1 m in the tucuxis, and the centroid frequency was 82.3 kHz in the botos and 93.1 kHz in the tucuxis. The high apparent source level and low centroid frequency are possibly due to the difference in body size or sound production organs, especially the nasal structure, the sound source of clicks in odontoceti.

Grafting polycaprolactone diol onto cellulose nanocrystals via click chemistry: Enhancing thermal stability and hydrophobic property.

PubMed

Zhou, Ling; He, Hui; Li, Mei-Chun; Huang, Siwei; Mei, Changtong; Wu, Qinglin

2018-06-01

Hydrophobic and thermally-stable cellulose nanocrystals (CNCs) were synthesized by polycarpolactone diol (PCL diol) grafting via click chemistry strategy. The synthesis was designed as a three-step procedure containing azide-modification of CNCs, alkyne-modification of PCL diol and sequent copper(I)-catalyzed azide-alkyne cycloaddition reaction. The structure of azide-modified CNCs and alkyne-modified PCL diol, the structure, hydrophobic ability and thermal stability of click products CNC-PCL were characterized. FTIR, XPS and H 1 NMR results indicated a successful grafting of the N 3 groups onto the CNCs, synthesis of PCL diol-CCH, and formation of the CNC-PCL material. CNC-PCL had enhanced dispersion in the non-polar solvent chloroform owing to the well-maintained microscale size and PCL-induced hydrophobic surface. The thermal stability of CNC-PCL was largely increased due to the grafting of thermally-stable PCL. This work demonstrates that click chemistry is an attractive modification strategy to graft CNCs with polyester chains for further potential application in polymer composites. Copyright © 2018 Elsevier Ltd. All rights reserved.

In Situ Forming, Cytocompatible, and Self-Recoverable Tough Hydrogels Based on Dual Ionic and Click Cross-Linked Alginate.

PubMed

Ghanian, Mohammad Hossein; Mirzadeh, Hamid; Baharvand, Hossein

2018-05-14

A dual cross-linking strategy was developed to answer the urgent need for fatigue-resistant, cytocompatible, and in situ forming tough hydrogels. Clickable, yet calcium-binding derivatives of alginate were synthesized by partial substitution of its carboxyl functionalities with furan, which could come into Diels-Alder click reaction with maleimide end groups of a four arm poly(ethylene glycol) cross-linker. Tuning the cooperative viscoelastic action of transient ionic and permanent click cross-links within the single network of alginate provided a soft tough hydrogel with a set of interesting features: (i) immediate self-recovery under cyclic loading, (ii) highly efficient and autonomous self-healing upon fracture, (iii) in situ forming ability for molding and minimally invasive injection, (iv) capability for viable cell encapsulation, and (v) reactivity for on-demand biomolecule conjugation. The facile strategy is applicable to a wide range of natural and synthetic polymers by introducing the calcium binding and click reacting functional groups and can broaden the use of tough hydrogels in load-bearing, cell-laden applications such as soft tissue engineering and bioactuators.

Highly sensitive fluorescence and SERS detection of azide through a simple click reaction of 8-chloroquinoline and phenylacetylene.

PubMed

Zeng, Qing; Ye, Lingling; Ma, Lu; Yin, Wenqing; Li, Tingsheng; Liang, Aihui; Jiang, Zhiliang

2015-05-01

In 0.19 mol/L acetic acid (HAc), a click reaction of 8-chloroquinoline/azide/phenylacetylene take places in aqueous solution without Cu(I) as a catalyst. 8-Chloroquinoline (CQN) exhibited a strong fluorescence peak at 430 nm that was quenched linearly as the concentration of azide increased from 20 to 1000 ng/mL. This quenching was due to consumption of CQN in the click reaction and a decrease in the number of efficiently excited photons due to the presence of triazole-quinoline ramification molecules with strong hydrophobicity. Using blue nanosilver sol as the substrate, CQN absorbed onto the surface of nanosilver particles, showing a strong surface-enhanced Raman scattering (SERS) peak at 1585 cm(-1) that decreased linearly as the azide concentration increased from 8 to 500 ng/mL; the detection limit was 4 ng/mL. Thus, two new, simple and sensitive fluorescence and SERS methods have been developed for the determination of azide via the click reaction. Copyright © 2014 John Wiley & Sons, Ltd.

Marine mammal tracks from two-hydrophone acoustic recordings made with a glider

NASA Astrophysics Data System (ADS)

Küsel, Elizabeth T.; Munoz, Tessa; Siderius, Martin; Mellinger, David K.; Heimlich, Sara

2017-04-01

A multinational oceanographic and acoustic sea experiment was carried out in the summer of 2014 off the western coast of the island of Sardinia, Mediterranean Sea. During this experiment, an underwater glider fitted with two hydrophones was evaluated as a potential tool for marine mammal population density estimation studies. An acoustic recording system was also tested, comprising an inexpensive, off-the-shelf digital recorder installed inside the glider. Detection and classification of sounds produced by whales and dolphins, and sometimes tracking and localization, are inherent components of population density estimation from passive acoustics recordings. In this work we discuss the equipment used as well as analysis of the data obtained, including detection and estimation of bearing angles. A human analyst identified the presence of sperm whale (Physeter macrocephalus) regular clicks as well as dolphin clicks and whistles. Cross-correlating clicks recorded on both data channels allowed for the estimation of the direction (bearing) of clicks, and realization of animal tracks. Insights from this bearing tracking analysis can aid in population density estimation studies by providing further information (bearings), which can improve estimates.

Click Chemistry for Analysis of Cell Proliferation in Flow Cytometry.

PubMed

Clarke, Scott T; Calderon, Veronica; Bradford, Jolene A

2017-10-02

The measurement of cellular proliferation is fundamental to the assessment of cellular health, genotoxicity, and the evaluation of drug efficacy. Labeling, detection, and quantification of cells in the synthesis phase of cell cycle progression are not only important for characterizing basic biology, but also in defining cellular responses to drug treatments. Changes in DNA replication during S-phase can provide valuable insights into mechanisms of cell growth, cell cycle kinetics, and cytotoxicity. A common method for detection of cell proliferation is the incorporation of a thymidine analog during DNA synthesis. This chapter presents a pulse labeling method using the thymidine analog, 5-ethynyl-2'-deoxyuridine (EdU), with subsequent detection by click chemistry. EdU detection using click chemistry is bio-orthogonal to most living systems and does not non-specifically label other biomolecules. Live cells are first pulsed with EdU. After antibody labeling cell surface markers, fixation, and permeabilization, the incorporated EdU is covalently labeled using click chemistry thereby identifying proliferating cells. Improvements in click chemistry allow for labeling in the presence of fluorescent proteins and phycobiliproteins without quenching due to copper. Measuring DNA replication during cell cycle progression has cell health applications in flow cytometry, fluorescence microscopy, and high content imaging. This protocol has been developed and optimized for research use only and is not suitable for use in diagnostic procedures. © 2017 by John Wiley & Sons, Inc. Copyright © 2017 John Wiley & Sons, Inc.

Improved Tumor Targeting and Longer Retention Time of NIR Fluorescent Probes Using Bioorthogonal Chemistry.

PubMed

Zhang, Xianghan; Wang, Bo; Zhao, Na; Tian, Zuhong; Dai, Yunpeng; Nie, Yongzhan; Tian, Jie; Wang, Zhongliang; Chen, Xiaoyuan

2017-01-01

The traditional labeling method for targeted NIR fluorescence probes requires directly covalent-bonded conjugation of targeting domains and fluorophores in vitro . Although this strategy works well, it is not sufficient for detecting or treating cancers in vivo , due to steric hindrance effects that relatively large fluorophore molecules exert on the configurations and physiological functions of specific targeting domains. The copper-free, "click-chemistry"-assisted assembly of small molecules in living systems may enhance tumor accumulation of fluorescence probes by improving the binding affinities of the targeting factors. Here, we employed a vascular homing peptide, GEBP11, as a targeting factor for gastric tumors, and we demonstrate its effectiveness for in vivo imaging via click-chemistry-mediated conjugation with fluorescence molecules in tumor xenograft mouse models. This strategy showed higher binding affinities than those of the traditional conjugation method, and our results showed that the tumor accumulation of click-chemistry-mediated probes are 11-fold higher than that of directly labeled probes. The tracking life was prolonged by 12-fold, and uptake of the probes into the kidney was reduced by 6.5-fold. For lesion tumors of different sizes, click-chemistry-mediated probes can achieve sufficient signal-to-background ratios (3.5-5) for in vivo detection, and with diagnostic sensitivity approximately 3.5 times that of traditional labeling probes. The click-chemistry-assisted detection strategy utilizes the advantages of "small molecule" probes while not perturbing their physiological functions; this enables tumor detection with high sensitivity and specific selectivity.

Studying Behaviors Among Neurosurgery Residents Using Web 2.0 Analytic Tools.

PubMed

Davidson, Benjamin; Alotaibi, Naif M; Guha, Daipayan; Amaral, Sandi; Kulkarni, Abhaya V; Lozano, Andres M

Web 2.0 technologies (e.g., blogs, social networks, and wikis) are increasingly being used by medical schools and postgraduate training programs as tools for information dissemination. These technologies offer the unique opportunity to track metrics of user engagement and interaction. Here, we employ Web 2.0 tools to assess academic behaviors among neurosurgery residents. We performed a retrospective review of all educational lectures, part of the core Neurosurgery Residency curriculum at the University of Toronto, posted on our teaching website (www.TheBrainSchool.net). Our website was developed using publicly available Web 2.0 platforms. Lecture usage was assessed by the number of clicks, and associations were explored with lecturer academic position, timing of examinations, and lecture/subspecialty topic. The overall number of clicks on 77 lectures was 1079. Most of these clicks were occurring during the in-training examination month (43%). Click numbers were significantly higher on lectures presented by faculty (mean = 18.6, standard deviation ± 4.1) compared to those delivered by residents (mean = 8.4, standard deviation ± 2.1) (p = 0.031). Lectures covering topics in functional neurosurgery received the most clicks (47%), followed by pediatric neurosurgery (22%). This study demonstrates the value of Web 2.0 analytic tools in examining resident study behavior. Residents tend to "cram" by downloading lectures in the same month of training examinations and display a preference for faculty-delivered lectures. Copyright © 2017 Association of Program Directors in Surgery. Published by Elsevier Inc. All rights reserved.

Dolphin hearing during echolocation: evoked potential responses in an Atlantic bottlenose dolphin (Tursiops truncatus).

PubMed

Li, Songhai; Nachtigall, Paul E; Breese, Marlee

2011-06-15

Auditory evoked potential (AEP) responses were recorded during echolocation in an Atlantic bottlenose dolphin (Tursiops truncatus) trained to accept suction-cup EEG electrodes and detect targets by echolocation. AEP recording was triggered by the echolocation clicks of the animal. Three targets with target strengths of -34, -28 and -22 dB were used at a target distance of 2 to 6.5 m for each target. The results demonstrated that the AEP appeared to both outgoing echolocation clicks and echoes during echolocation, with AEP complexes consisting of alternative positive and negative waves. The echo-related AEP amplitudes were obviously lower than the outgoing click-related AEP amplitudes for all the targets at the investigated target distances. However, for targets with target strengths of -22 and -28 dB, the peak-to-peak amplitudes of the echo-related AEPs were dependent on the target distances. The echo-related AEP response amplitudes increased at further target distances, demonstrating an overcompensation of echo attenuation with target distance in the echo-perception system of the dolphin biosonar. Measurement and analysis of outgoing click intensities showed that the click levels increased with target distance (R) by a factor of approximately 10 to 17.5 logR depending on target strength. The results demonstrated that a dual-component biosonar control system formed by intensity compensation behavior in both the transmission and receiving phases of a biosonar cycle exists synchronously in the dolphin biosonar system.

Fast and flexible selection with a single switch.

PubMed

Broderick, Tamara; MacKay, David J C

2009-10-22

Selection methods that require only a single-switch input, such as a button click or blink, are potentially useful for individuals with motor impairments, mobile technology users, and individuals wishing to transmit information securely. We present a single-switch selection method, "Nomon," that is general and efficient. Existing single-switch selection methods require selectable options to be arranged in ways that limit potential applications. By contrast, traditional operating systems, web browsers, and free-form applications (such as drawing) place options at arbitrary points on the screen. Nomon, however, has the flexibility to select any point on a screen. Nomon adapts automatically to an individual's clicking ability; it allows a person who clicks precisely to make a selection quickly and allows a person who clicks imprecisely more time to make a selection without error. Nomon reaps gains in information rate by allowing the specification of beliefs (priors) about option selection probabilities and by avoiding tree-based selection schemes in favor of direct (posterior) inference. We have developed both a Nomon-based writing application and a drawing application. To evaluate Nomon's performance, we compared the writing application with a popular existing method for single-switch writing (row-column scanning). Novice users wrote 35% faster with the Nomon interface than with the scanning interface. An experienced user (author TB, with 10 hours practice) wrote at speeds of 9.3 words per minute with Nomon, using 1.2 clicks per character and making no errors in the final text.

1,2,3-Triazole-Heme Interactions in Cytochrome P450: Functionally Competent Triazole-Water- Heme Complexes

PubMed Central

Conner, Kip P.; Vennam, Preethi; Woods, Caleb M.; Krzyaniak, Matthew D.; Bowman, Michael K.; Atkins, William M.

2012-01-01

In comparison to imidazole (IMZ) and 1,2,4-triazole (1,2,4-TRZ) the isosteric 1,2,3-triazole (1,2,3-TRZ) is unrepresented among CYP inhibitors. This is surprising because 1,2,3-TRZs are easily obtained via ‘click’ chemistry. To understand this underrepresentation of 1,2,3-TRZs among CYP inhibitors, thermodynamic and DFT computational studies were performed with unsusbstituted IMZ, 1,2,4-TRZ, and 1,2,3-TRZ. The results indicate that the lower affinity of 1,2,3-TRZ for the heme iron includes a large unfavorable entropy term likely originating in solvent – 1,2,3-TRZ interactions; the difference is not solely due to differences in the enthalpy of heme – ligand interactions. In addition, the 1,2,3-TRZ fragment was incorporated into a well-established CYP3A4 substrate and mechanism based inactivator, 17-α-ethynylestradiol (17EE), via click chemistry. This derivative, 17-click, yielded optical spectra consistent with low spin ferric heme iron (type II) in contrast to 17EE, which yields a high spin complex (type I). Furthermore, the rate of CYP3A4-mediated metabolism of 17-click was comparable to 17EE, and with different regioselectivity. Surprisingly, CW EPR and HYSCORE EPR spectroscopy indicate that the 17-click does not displace water from the 6th axial ligand position of CYP3A4 as expected for a type II ligand. We propose a binding model where 17-click pendant 1,2,3-TRZ hydrogen bonds with the 6th axial water ligand. The results demonstrate the potential for 1,2,3-TRZ to form metabolically labile water-bridged low spin heme complexes, consistent with recent evidence that nitrogenous type II ligands of CYPs can be efficiently metabolized. The specific case of [CYP3A4•17-click] highlights the risk of interpreting CYP-ligand complex structure on the basis of optical spectra. PMID:22809252

A new microfluidic approach for the one-step capture, amplification and label-free quantification of bacteria from raw samples† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc03880h Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file.

PubMed Central

Pereiro, Iago; Bendali, Amel; Tabnaoui, Sanae; Alexandre, Lucile; Srbova, Jana; Bilkova, Zuzana; Deegan, Shane; Joshi, Lokesh; Viovy, Jean-Louis; Malaquin, Laurent

2017-01-01

A microfluidic method to specifically capture and detect infectious bacteria based on immunorecognition and proliferative power is presented. It involves a microscale fluidized bed in which magnetic and drag forces are balanced to retain antibody-functionalized superparamagnetic beads in a chamber during sample perfusion. Captured cells are then cultivated in situ by infusing nutritionally-rich medium. The system was validated by the direct one-step detection of Salmonella Typhimurium in undiluted unskimmed milk, without pre-treatment. The growth of bacteria induces an expansion of the fluidized bed, mainly due to the volume occupied by the newly formed bacteria. This expansion can be observed with the naked eye, providing simple low-cost detection of only a few bacteria and in a few hours. The time to expansion can also be measured with a low-cost camera, allowing quantitative detection down to 4 cfu (colony forming unit), with a dynamic range of 100 to 107 cfu ml–1 in 2 to 8 hours, depending on the initial concentration. This mode of operation is an equivalent of quantitative PCR, with which it shares a high dynamic range and outstanding sensitivity and specificity, operating at the live cell rather than DNA level. Specificity was demonstrated by controls performed in the presence of a 500× excess of non-pathogenic Lactococcus lactis. The system's versatility was demonstrated by its successful application to the detection and quantitation of Escherichia coli O157:H15 and Enterobacter cloacae. This new technology allows fast, low-cost, portable and automated bacteria detection for various applications in food, environment, security and clinics. PMID:28626552

Generation of human auditory steady-state responses (SSRs). II: Addition of responses to individual stimuli.

PubMed

Santarelli, R; Maurizi, M; Conti, G; Ottaviani, F; Paludetti, G; Pettorossi, V E

1995-03-01

In order to investigate the generation of the 40 Hz steady-state response (SSR), auditory potentials evoked by clicks were recorded in 16 healthy subjects in two stimulating conditions. Firstly, repetition rates of 7.9 and 40 Hz were used to obtain individual middle latency responses (MLRs) and 40 Hz-SSRs, respectively. In the second condition, eight click trains were presented at a 40 Hz repetition rate and an inter-train interval of 126 ms. We extracted from the whole train response: (1) the response-segment taking place after the last click of the train (last click response, LCR), (2) a modified LCR (mLCR) obtained by clearing the LCR from the amplitude enhancement due to the overlapping of the responses to the clicks preceding the last within the stimulus train. In comparison to MLRs, the most relevant feature of the evoked activity following the last click of the train (LCRs, mLCRs) was the appearance in the 50-110 ms latency range of one (in 11 subjects) or two (in 2 subjects) additional positive-negative deflections having the same periodicity as that of MLR waves. The grand average (GA) of the 40 Hz-SSRs was compared with three predictions synthesized by superimposing: (1) the GA of MLRs, (2) the GA of LCRs, (3) the GA of mLCRs. Both the MLR and mLCR predictions reproduced the recorded signal in amplitude while the LCR prediction amplitude resulted almost twice that of the 40 Hz-SSR. With regard to the phase, the MLR, LCR and mLCR closely predicted the recorded signal. Our findings confirm the effectiveness of the linear addition mechanism in the generation of the 40 Hz-SSR. However the responses to individual stimuli within the 40 Hz-SSR differ from MLRs because of additional periodic activity. These results suggest that phenomena related to the resonant frequency of the activated system may play a role in the mechanisms which interact to generate the 40 Hz-SSR.

Impact of a social media campaign targeting men who have sex with men during an outbreak of syphilis in Winnipeg, Canada.

PubMed

Ross, C; Shaw, S; Marshall, S; Stephen, S; Bailey, K; Cole, R; Wylie, J; Bullard, J; Van Caeseele, P; Reimer, J; Plourde, P

2016-02-04

The city of Winnipeg has experienced a surge of infectious syphilis cases since the fall of 2012, concentrated among men who have sex with men (MSM) and who use social media technologies-including phone applications-to meet sexual contacts. To evaluate the acceptability, cost and effectiveness of a campaign promoting syphilis testing on popular websites and applications used by MSM in the Winnipeg Health Region (WHR). The Winnipeg Regional Health Authority developed a campaign in March 2014 highlighting the syphilis outbreak and the importance of seeking testing. Over one month, advertisements appeared on four web-platforms: Grindr, Facebook, Squirt and the Gay Ad Network. When clicked, ads would direct the user to an information website. Acceptability was assessed using the number of 'clicks' elicited by advertisements on each platform. The cost of each platform's run of advertisements was compared to the number clicks elicited to produce a cost-per-click ratio for each platform. Effectiveness was assessed by comparing the number of syphilis tests ordered for male residents of the Winnipeg Health Region in the seven-week period before and after the campaign, as well as to the same time periods in 2012 and 2013. Out of 800,000 appearances purchased, the advertisements elicited 2,166 clicks, suggesting good acceptability. Grindr and Squirt advertisements had a better cost-per-click ratio than Facebook or the Gay Ad Network. There was no significant difference in testing before (2,049 tests) versus after (2,025 tests) the campaign and these findings were similar to testing trends in 2012 and 2013. Although this web-based campaign showed good acceptability and low cost, it did not appear to increase syphilis testing. This may be due to a poor campaign design; it also suggests that an education campaign alone may be insufficient to change behaviour.

The New Planetary Science Archive (PSA): Exploration and Discovery of Scientific Datasets from ESA's Planetary Missions

NASA Astrophysics Data System (ADS)

Heather, David; Besse, Sebastien; Vallat, Claire; Barbarisi, Isa; Arviset, Christophe; De Marchi, Guido; Barthelemy, Maud; Coia, Daniela; Costa, Marc; Docasal, Ruben; Fraga, Diego; Grotheer, Emmanuel; Lim, Tanya; MacFarlane, Alan; Martinez, Santa; Rios, Carlos; Vallejo, Fran; Saiz, Jaime

2017-04-01

The Planetary Science Archive (PSA) is the European Space Agency's (ESA) repository of science data from all planetary science and exploration missions. The PSA provides access to scientific datasets through various interfaces at http://psa.esa.int. All datasets are scientifically peer-reviewed by independent scientists, and are compliant with the Planetary Data System (PDS) standards. The PSA is currently implementing a number of significant improvements, mostly driven by the evolution of the PDS standard, and the growing need for better interfaces and advanced applications to support science exploitation. As of the end of 2016, the PSA is hosting data from all of ESA's planetary missions. This includes ESA's first planetary mission Giotto that encountered comet 1P/Halley in 1986 with a flyby at 800km. Science data from Venus Express, Mars Express, Huygens and the SMART-1 mission are also all available at the PSA. The PSA also contains all science data from Rosetta, which explored comet 67P/Churyumov-Gerasimenko and asteroids Steins and Lutetia. The year 2016 has seen the arrival of the ExoMars 2016 data in the archive. In the upcoming years, at least three new projects are foreseen to be fully archived at the PSA. The BepiColombo mission is scheduled for launch in 2018. Following that, the ExoMars Rover Surface Platform (RSP) in 2020, and then the JUpiter ICy moon Explorer (JUICE). All of these will archive their data in the PSA. In addition, a few ground-based support programmes are also available, especially for the Venus Express and Rosetta missions. The newly designed PSA will enhance the user experience and will significantly reduce the complexity for users to find their data promoting one-click access to the scientific datasets with more customized views when needed. This includes a better integration with Planetary GIS analysis tools and Planetary interoperability services (search and retrieve data, supporting e.g. PDAP, EPN-TAP). It will also be up-to-date with versions 3 and 4 of the PDS standards, as PDS4 will be used for ESA's ExoMars and upcoming BepiColombo missions. Users will have direct access to documentation, information and tools that are relevant to the scientific use of the dataset, including ancillary datasets, Software Interface Specification (SIS) documents, and any tools/help that the PSA team can provide. The new PSA interface was released in January 2017. The home page provides a direct and simple access to the scientific data, aiming to help scientists to discover and explore its content. The archive can be explored through a set of parameters that allow the selection of products through space and time. Quick views provide information needed for the selection of appropriate scientific products. During 2017, the PSA team will focus their efforts on developing a map search interface using GIS technologies to display ESA planetary datasets, an image gallery providing navigation through images to explore the datasets, and interoperability with international partners. This will be done in parallel with additional metadata searchable through the interface (i.e., geometry), and with a dedication to improve the content of 20 years of space exploration.

Effect of High-Pass Filtering on the Neonatal Auditory Brainstem Response to Air- and Bone-Conducted Clicks.

ERIC Educational Resources Information Center

Stuart, Andrew; Yang, Edward Y.

1994-01-01

Simultaneous 3- channel recorded auditory brainstem responses (ABR) were obtained from 20 neonates with various high-pass filter settings and low intensity levels. Results support the advocacy of less restrictive high-pass filtering for neonatal and infant ABR screening to air-conducted and bone-conducted clicks. (Author/JDD)

Effectiveness of the May 2005 rural demonstration program and the Click It or Ticket mobilization in the Great Lakes region : first year results

DOT National Transportation Integrated Search

2007-04-01

NHTSAs Great Lakes Region (GLR) implemented a Rural Demonstration Program (RDP) prior to the May 2005 Click It or Ticket (CIOT) mobilization with the goal of increasing seat belt usage in rural areas. Paid media was used to notify rural residents ...

Brick and Click Libraries: Proceedings of an Academic Library Symposium (11th, Maryville, Missouri, November 4, 2011)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.

2011-01-01

Twenty-three scholarly papers and twelve abstracts comprise the content of the eleventh annual Brick and Click Libraries Symposium, held at Northwest Missouri State University in Maryville, Missouri. The peer-reviewed proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of…

78 FR 64905 - Carriage of Conditionally Permitted Shale Gas Extraction Waste Water in Bulk

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78 FR 63290 - Qualification of Drivers; Exemption Applications; Vision

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Brick and Click Libraries: Proceedings of an Academic Library Symposium (10th, Maryville, Missouri, November 5, 2010)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Ury, Connie Jo, Ed.; Park, Sarah G., Ed.

2010-01-01

Twenty-one scholarly papers and fifteen abstracts comprise the content of the tenth annual Brick and Click Libraries Symposium, held annually at Northwest Missouri State University in Maryville, Missouri. The peer-reviewed proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of…

Understanding Microbial Sensing in Inflammatory Bowel Disease Using Click Chemistry

DTIC Science & Technology

2017-10-01

pathogens and commensals. However, the technology available to track these molecules in host cells and tissues remains primitive. To address this...live, luminal bacteria into specific host intestinal immune cells and their subsequent degradation in host phagocytes. Notably, this approach also...click-chemistry, bacterial cell wall, bacterial outer membrane, peptidoglycan, lipopolysaccharide, endotoxin, capsular polysaccharide, inflammatory

Understanding Microbial Sensing in Inflammatory Bowel Disease Using Click Chemistry

DTIC Science & Technology

2017-10-01

both pathogens and commensals. However, the technology available to track these molecules in host cells and tissues remains primitive. To address this...from live, luminal bacteria into specific host intestinal immune cells and their subsequent degradation in host phagocytes. Notably, this approach...Bioorthogonal click-chemistry, bacterial cell wall, bacterial outer membrane, peptidoglycan, lipopolysaccharide, endotoxin, capsular polysaccharide

Climate Forecast System

Science.gov Websites

Skip Navigation Links www.nws.noaa.gov NOAA logo - Click to go to the NOAA home page National Weather Service NWS logo - Click to go to the NWS home page Climate Forecast System Home News Organization Search : Go Search Go CFS Home CFS version 2 News Documentation Downloads Reanalysis CFSv2 at CPC CFS

78 FR 2225 - Special Local Regulation; 2013 Lauderdale Air Show, Atlantic Ocean; Fort Lauderdale, FL

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2013-01-10

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Brick and Click Libraries: Proceedings of an Academic Libraries Symposium (8th, Maryville, Missouri, November 7, 2008)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Ury, Connie Jo, Ed.; Park, Sarah G., Ed.

2008-01-01

Eighteen scholarly papers and eighteen abstracts comprise the content of the 8th "Brick and Click Libraries Symposium," held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship. Many of the…

76 FR 30887 - Special Local Regulations for Marine Events, Bogue Sound; Morehead City, NC

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77 FR 6039 - Special Local Regulations; Savannah Tall Ships Challenge, Savannah River, Savannah, GA

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Joint Chiefs of Staff > About > Joint Staff Inspector General

Science.gov Websites

DD Form 2949, Joint Inspector General Action Request (available at the link below). However, if you action if you intentionally make false statements. When you sign a DD Form 2949, you are signing a . DD Form 2949, Joint Inspector General Action Request Click here to expand content Click here to

Effects of Computer Skill on Mouse Move and Click Performance

ERIC Educational Resources Information Center

Panagiotakopoulos, Chris; Sarris, Menelaos

2008-01-01

This study focuses on the use of computers in the field of education. It reports a series of experimental mouse move and click tasks on constant and moving stimuli. These experiments attempt to explore the efficiency with which individuals of different skill level and age group perform using a mouse. Differences in performance between high-skill…

Brick and Click Libraries: Proceedings of an Academic Library Symposium (9th, Maryville, Missouri, November 6, 2009)

ERIC Educational Resources Information Center

Ury, Connie Jo, Ed.; Baudino, Frank, Ed.; Park, Sarah G., Ed.

2009-01-01

Twenty-one scholarly papers and fourteen abstracts comprise the content of the ninth annual "Brick and Click Libraries Symposium," held annually at Northwest Missouri State University in Maryville, Missouri. The peer-reviewed proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future…

Brick and Click Libraries: An Academic Library Symposium (12th, Maryville, Missouri, October 26, 2012)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Johnson, Carolyn, Ed.

2012-01-01

Twenty scholarly papers and fifteen abstracts comprise the content of the twelfth annual Brick and Click Libraries Symposium, held at Northwest Missouri State University in Maryville, Missouri. The peer-reviewed proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship.…

CLICK: Arts Education and Critical Social Dialogue within Global Youth Work Practice

ERIC Educational Resources Information Center

Aubrey, Meg

2015-01-01

This article discusses CLICK, a collaborative theatre project between the Mess Up The Mess Theatre Company in Wales, the Hong Kong Academy for Performing Arts, the Australian Theatre for Young People, and Inspired Productions in New Zealand. This case study demonstrates the value of using arts education to bring together young people from multiple…

Solvent-resistant nanofiltration for product purification and catalyst recovery in click chemistry reactions.

PubMed

Cano-Odena, Angels; Vandezande, Pieter; Fournier, David; Van Camp, Wim; Du Prez, Filip E; Vankelecom, Ivo F J

2010-01-18

The quickly developing field of "click" chemistry would undoubtedly benefit from the availability of an easy and efficient technology for product purification to reduce the potential health risks associated with the presence of copper in the final product. Therefore, solvent-resistant nanofiltration (SRNF) membranes have been developed to selectively separate "clicked" polymers from the copper catalyst and solvent. By using these solvent-stable cross-linked polyimide membranes in diafiltration, up to 98 % of the initially present copper could be removed through the membrane together with the DMF solvent, the polymer product being almost completely retained. This paper also presents the first SRNF application in which the catalyst permeates through the membrane and the reaction product is retained.

Click polymerization for the synthesis of reduction-responsive polymeric prodrug

NASA Astrophysics Data System (ADS)

Zhang, Xiaojin; Wang, Hongquan; Dai, Yu

2018-05-01

Click polymerization is a powerful polymerization technique for the construction of new macromolecules with well-defined structures and multifaceted functionalities. Here, we synthesize reduction-responsive polymeric prodrug PEG- b-(PSS- g-MTX)- b-PEG containing disulfide bonds and pendant methotrexate (MTX) via two-step click polymerization followed by conjugating MTX to pendant hydroxyl. MTX content in polymeric prodrug is 13.5%. Polymeric prodrug is able to form polymeric micelles by self-assembly in aqueous solution. Polymeric micelles are spherical nanoparticles with tens of nanometers in size. Of note, polymeric micelles are reduction-responsive due to disulfide bonds in the backbone of PEG- b-(PSS- g-MTX)- b-PEG and could release pendant drugs in the presence of the reducing agents such as dl-dithiothreitol (DTT).

The beluga whale produces two pulses to form its sonar signal

PubMed Central

Lammers, Marc O.; Castellote, Manuel

2009-01-01

Odontocete cetaceans use biosonar clicks to acoustically probe their aquatic environment with an aptitude unmatched by man-made sonar. A cornerstone of this ability is their use of short, broadband pulses produced in the region of the upper nasal passages. Here we provide empirical evidence that a beluga whale (Delphinapterus leucas) uses two signal generators simultaneously when echolocating. We show that the pulses of the two generators are combined as they are transmitted through the melon to produce a single echolocation click emitted from the front of the animal. Generating two pulses probably offers the beluga the ability to control the energy and frequency distribution of the emitted click and may allow it to acoustically steer its echolocation beam. PMID:19324643

Fast dye salts provide fast access to azidoarene synthons in multi-step one-pot tandem click transformations

PubMed Central

Fletcher, James T.; Reilly, Jacquelline E.

2012-01-01

This study examined whether commercially available diazonium salts could be used as efficient aromatic azide precursors in one-pot multi-step click transformations. Seven different diazonium salts, including Fast Red RC, Fast Blue B, Fast Corinth V and Variamine Blue B were surveyed under aqueous click reaction conditions of CuSO4/Na ascorbate catalyst with 1:1 t-BuOH:H2O solvent. Two-step tandem reactions with terminal alkyne and diyne co-reactants led to 1,2,3-triazole products in 66%-88% yields, while three-step tandem reactions with trimethylsilyl-protected alkyne and diyne co-reactants led to 1,2,3-triazole products in 61%-78% yields. PMID:22368306

The effect of channel deepening on tides and storm surge: A case study of Wilmington, NC

NASA Astrophysics Data System (ADS)

Familkhalili, R.; Talke, S. A.

2016-09-01

In this study we investigate the hypothesis that increasing channel depth in estuaries can amplify both tides and storm surge by developing an idealized numerical model representing the 1888, 1975, and 2015 bathymetric conditions of the Cape Fear River Estuary, NC. Archival tide gauge data recovered from the U.S. National Archives indicates that mean tidal range in Wilmington has doubled to 1.55 m since the 1880s, with a much smaller increase of 0.07 m observed near the ocean boundary. These tidal changes are reproduced by simulating channel depths of 7 m (1888 condition) and 15.5 m (modern condition). Similarly, model sensitivity studies using idealized, parametric tropical cyclones suggest that the storm surge in the worst-case, CAT-5 event may have increased from 3.8 ± 0.25 m to 5.6 ± 0.6 m since the nineteenth century. The amplification in both tides and storm surge is influenced by reduced hydraulic drag caused by greater mean depths.

Unravelling the mechanisms of vibrational relaxation in solution† †All experimental data are archived in the University of Bristol's Research Data Storage Facility (DOI: 10.5523/bris.2vk036f35m5aq2dnlb79c0wcsh). ‡ ‡Electronic supplementary information (ESI) available: Further discussion of spectral lineshapes, concentration dependence of transient absorption data, theoretical calculations, IR-pump IR-probe spectra, transient absorption spectra including animation of spectra. See DOI: 10.1039/c6sc05234g Click here for additional data file. Click here for additional data file.

PubMed Central

Grubb, Michael P.; Coulter, Philip M.; Marroux, Hugo J. B.

2017-01-01

We present a systematic study of the mode-specific vibrational relaxation of NO2 in six weakly-interacting solvents (perfluorohexane, perfluoromethylcyclohexane, perfluorodecalin, carbon tetrachloride, chloroform, and d-chloroform), chosen to elucidate the dominant energy transfer mechanisms in the solution phase. Broadband transient vibrational absorption spectroscopy has allowed us to extract quantum state-resolved relaxation dynamics of the two distinct NO2 fragments produced from the 340 nm photolysis of N2O4 → NO2(X) + NO2(A) and their separate paths to thermal equilibrium. Distinct relaxation pathways are observed for the NO2 bending and stretching modes, even at energies as high as 7000 cm–1 above the potential minimum. Vibrational energy transfer is governed by different interaction mechanisms in the various solvent environments, and proceeds with timescales ranging from 20–1100 ps. NO2 relaxation rates in the perfluorocarbon solvents are identical despite differences in acceptor mode state densities, infrared absorption cross sections, and local solvent structure. Vibrational energy is shown to be transferred to non-vibrational solvent degrees of freedom (V-T) through impulsive collisions with the perfluorocarbon molecules. Conversely, NO2 relaxation in chlorinated solvents is reliant on vibrational resonances (V-V) while V-T energy transfer is inefficient and thermal excitation of the surrounding solvent molecules inhibits faster vibrational relaxation through direct complexation. Intramolecular vibrational redistribution allows the symmetric stretch of NO2 to act as a gateway for antisymmetric stretch energy to exit the molecule. This study establishes an unprecedented level of detail for the cooling dynamics of a solvated small molecule, and provides a benchmark system for future theoretical studies of vibrational relaxation processes in solution. PMID:28451375

Trends of brominated diphenyl ethers in fresh and archived Great Lakes fish (1979-2005)

USGS Publications Warehouse

Batterman, Stuart; Chernyak, Sergei; Gwynn, Erica; Cantonwine, David; Jia, Chunrong; Begnoche, Linda J.; Hickey, James P.

2007-01-01

While few environmental measurements of brominated diphenyl ethers (BDEs) were completed prior to the mid-1990s, analysis of appropriately archived samples might enable the determination of contaminant trends back to the introduction of these chemicals. In this paper, we first investigate the stability of BDEs in archived frozen and extracted fish samples, and then characterize trends of these chemicals in rainbow smelt (Osmerus mordax) and lake trout (Salvelinus namaycush) in each of the Great Lakes between 1979 and 2005. We focus on the four most common congeners (BDE-47, 100, 99 and 153) and use a change-point analysis to detect shifts in trends. Analyses of archived fish samples yielded precise BDE concentration measurements with only small losses (0.8% per year in frozen fish tissues, 2.2% per year in refrigerated extracts). Trends in fish from all Great Lakes showed large increases in BDE concentrations that started in the early to mid-1980s with fairly consistent doubling times (generally 2–4 years except in Lake Erie smelt where levels increased very slowly), though concentrations and trends show differences by congener, fish species and lake. The most recent data show that accumulation rates are slowing, and concentrations of penta- and hexa-congeners in trout from Lakes Ontario and Michigan and smelt from Lake Ontario started to decrease in the mid-1990s. Trends in smelt and trout are evolving somewhat differently, and trout concentrations in the five lakes are now ranked as Michigan > Superior = Ontario > Huron = Erie, and smelt concentrations as Michigan > Ontario > Huron > Superior > Erie. The analysis of properly archived samples permits the reconstruction of historical trends, congener distributions, biomagnification and other information that can aid the understanding and management of these contaminants.

The global Landsat archive: Status, consolidation, and direction

USGS Publications Warehouse

Wulder, Michael A.; White, Joanne C.; Loveland, Thomas; Woodcock, Curtis; Belward, Alan; Cohen, Warren B.; Fosnight, Eugene A.; Shaw, Jerad; Masek, Jeffery G.; Roy, David P.

2016-01-01

New and previously unimaginable Landsat applications have been fostered by a policy change in 2008 that made analysis-ready Landsat data free and open access. Since 1972, Landsat has been collecting images of the Earth, with the early years of the program constrained by onboard satellite and ground systems, as well as limitations across the range of required computing, networking, and storage capabilities. Rather than robust on-satellite storage for transmission via high bandwidth downlink to a centralized storage and distribution facility as with Landsat-8, a network of receiving stations, one operated by the U.S. government, the other operated by a community of International Cooperators (ICs), were utilized. ICs paid a fee for the right to receive and distribute Landsat data and over time, more Landsat data was held outside the archive of the United State Geological Survey (USGS) than was held inside, much of it unique. Recognizing the critical value of these data, the USGS began a Landsat Global Archive Consolidation (LGAC) initiative in 2010 to bring these data into a single, universally accessible, centralized global archive, housed at the Earth Resources Observation and Science (EROS) Center in Sioux Falls, South Dakota. The primary LGAC goals are to inventory the data held by ICs, acquire the data, and ingest and apply standard ground station processing to generate an L1T analysis-ready product. As of January 1, 2015 there were 5,532,454 images in the USGS archive. LGAC has contributed approximately 3.2 million of those images, more than doubling the original USGS archive holdings. Moreover, an additional 2.3 million images have been identified to date through the LGAC initiative and are in the process of being added to the archive. The impact of LGAC is significant and, in terms of images in the collection, analogous to that of having had twoadditional Landsat-5 missions. As a result of LGAC, there are regions of the globe that now have markedly improved Landsat data coverage, resulting in an enhanced capacity for mapping, monitoring change, and capturing historic conditions. Although future missions can be planned and implemented, the past cannot be revisited, underscoring the value and enhanced significance of historical Landsat data and the LGAC initiative. The aim of this paper is to report the current status of the global USGS Landsat archive, document the existing and anticipated contributions of LGAC to the archive, and characterize the current acquisitions of Landsat-7 and Landsat-8. Landsat-8 is adding data to the archive at an unprecedented rate as nearly all terrestrial images are now collected. We also offer key lessons learned so far from the LGAC initiative, plus insights regarding other critical elements of the Landsat program looking forward, such as acquisition, continuity, temporal revisit, and the importance of continuing to operationalize the Landsat program.

The CUORE slow monitoring systems

DOE PAGES

Gladstone, L.; Biare, D.; Cappelli, L.; ...

2017-09-20

CUORE is a cryogenic experiment searching primarily for neutrinoless double decay inmore » $$^{130}$$Te. It will begin data-taking operations in 2016. To monitor the cryostat and detector during commissioning and data taking, we have designed and developed Slow Monitoring systems. In addition to real-time systems using LabVIEW, we have an alarm, analysis, and archiving website that uses MongoDB, AngularJS, and Bootstrap software. These modern, state of the art software packages make the monitoring system transparent, easily maintainable, and accessible on many platforms including mobile devices.« less

Photographic copy Photograph of 1892 drawing by artist H.W. Locket. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Photographic copy Photograph of 1892 drawing by artist H.W. Locket. Original Photographer Unknown. Loose in oversized box located at the National Museum of American History, Smithsonian Institution, Archives Center, Work and Industry Division, Washington, D.C. EARLY DESIGN OF “DOUBLE TRACK RAILROAD BRIDGE OVER MISSISSIPPI RIVER ABOVE THE CITY OF NEW ORLEANS, LA, U.S.A. TO BE BUILT BY SOUTHERN BRIDGE AND RAILWAY COMPANY”. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

The CUORE slow monitoring systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gladstone, L.; Biare, D.; Cappelli, L.

CUORE is a cryogenic experiment searching primarily for neutrinoless double decay inmore » $$^{130}$$Te. It will begin data-taking operations in 2016. To monitor the cryostat and detector during commissioning and data taking, we have designed and developed Slow Monitoring systems. In addition to real-time systems using LabVIEW, we have an alarm, analysis, and archiving website that uses MongoDB, AngularJS, and Bootstrap software. These modern, state of the art software packages make the monitoring system transparent, easily maintainable, and accessible on many platforms including mobile devices.« less

A Speckle survey of Southern Hipparcos Visual Doubles and Geneva-Copenhagen Spectroscopic Binaries

NASA Astrophysics Data System (ADS)

Mendez, R. A.; Tokovinin, A.; Horch, E.

2018-01-01

We present a speckle survey of Hipparcos visual doubles and spectroscopic binary stars identified by the Geneva-Copenhagen spectroscopic survey with the SOAR 4m telescope + HRCam. These systems represent our best chance to take advantage of Gaia parallaxes for the purpose of stellar mass determinations. Many of these systems already have mass fractions (although generally no spectroscopic orbit - an astrometric orbit will determine individual masses), metallicity information, and Hipparcos distances. They will be used to improve our knowledge of the mass-luminosity relation, particularly for lower-metallicity stars. Our survey will create the first all-sky, volume-limited, speckle archive for the two primary samples, complementing a similar effort that has been recently been completed at the WIYN 3.5-m telescope in the Northern Hemisphere. This extension to the Southern Hemisphere will fill out the picture for a wider metallicity range.

Clicking in shallow rivers: short-range echolocation of Irrawaddy and Ganges River dolphins in a shallow, acoustically complex habitat.

PubMed

Jensen, Frants H; Rocco, Alice; Mansur, Rubaiyat M; Smith, Brian D; Janik, Vincent M; Madsen, Peter T

2013-01-01

Toothed whales (Cetacea, odontoceti) use biosonar to navigate their environment and to find and catch prey. All studied toothed whale species have evolved highly directional, high-amplitude ultrasonic clicks suited for long-range echolocation of prey in open water. Little is known about the biosonar signals of toothed whale species inhabiting freshwater habitats such as endangered river dolphins. To address the evolutionary pressures shaping the echolocation signal parameters of non-marine toothed whales, we investigated the biosonar source parameters of Ganges river dolphins (Platanista gangetica gangetica) and Irrawaddy dolphins (Orcaella brevirostris) within the river systems of the Sundarban mangrove forest. Both Ganges and Irrawaddy dolphins produced echolocation clicks with a high repetition rate and low source level compared to marine species. Irrawaddy dolphins, inhabiting coastal and riverine habitats, produced a mean source level of 195 dB (max 203 dB) re 1 µPapp whereas Ganges river dolphins, living exclusively upriver, produced a mean source level of 184 dB (max 191) re 1 µPapp. These source levels are 1-2 orders of magnitude lower than those of similar sized marine delphinids and may reflect an adaptation to a shallow, acoustically complex freshwater habitat with high reverberation and acoustic clutter. The centroid frequency of Ganges river dolphin clicks are an octave lower than predicted from scaling, but with an estimated beamwidth comparable to that of porpoises. The unique bony maxillary crests found in the Platanista forehead may help achieve a higher directionality than expected using clicks nearly an octave lower than similar sized odontocetes.

Clicking in Shallow Rivers: Short-Range Echolocation of Irrawaddy and Ganges River Dolphins in a Shallow, Acoustically Complex Habitat

PubMed Central

Jensen, Frants H.; Rocco, Alice; Mansur, Rubaiyat M.; Smith, Brian D.; Janik, Vincent M.; Madsen, Peter T.

2013-01-01

Toothed whales (Cetacea, odontoceti) use biosonar to navigate their environment and to find and catch prey. All studied toothed whale species have evolved highly directional, high-amplitude ultrasonic clicks suited for long-range echolocation of prey in open water. Little is known about the biosonar signals of toothed whale species inhabiting freshwater habitats such as endangered river dolphins. To address the evolutionary pressures shaping the echolocation signal parameters of non-marine toothed whales, we investigated the biosonar source parameters of Ganges river dolphins (Platanista gangetica gangetica) and Irrawaddy dolphins (Orcaella brevirostris) within the river systems of the Sundarban mangrove forest. Both Ganges and Irrawaddy dolphins produced echolocation clicks with a high repetition rate and low source level compared to marine species. Irrawaddy dolphins, inhabiting coastal and riverine habitats, produced a mean source level of 195 dB (max 203 dB) re 1 µPapp whereas Ganges river dolphins, living exclusively upriver, produced a mean source level of 184 dB (max 191) re 1 µPapp. These source levels are 1–2 orders of magnitude lower than those of similar sized marine delphinids and may reflect an adaptation to a shallow, acoustically complex freshwater habitat with high reverberation and acoustic clutter. The centroid frequency of Ganges river dolphin clicks are an octave lower than predicted from scaling, but with an estimated beamwidth comparable to that of porpoises. The unique bony maxillary crests found in the Platanista forehead may help achieve a higher directionality than expected using clicks nearly an octave lower than similar sized odontocetes. PMID:23573197

Aural Acoustic Stapedius-Muscle Reflex Threshold Procedures to Test Human Infants and Adults.

PubMed

Keefe, Douglas H; Feeney, M Patrick; Hunter, Lisa L; Fitzpatrick, Denis F

2017-02-01

Power-based procedures are described to measure acoustic stapedius-muscle reflex threshold and supra-threshold responses in human adult and infant ears at frequencies from 0.2 to 8 kHz. The stimulus set included five clicks in which four pulsed activators were placed between each pair of clicks, with each stimulus set separated from the next by 0.79 s to allow for reflex decay. Each click response was used to detect the presence of reflex effects across frequency that were elicited by a pulsed broadband-noise or tonal activator in the ipsilateral or contralateral test ear. Acoustic reflex shifts were quantified in terms of the difference in absorbed sound power between the initial baseline click and the later four clicks in each set. Acoustic reflex shifts were measured over a 40-dB range of pulsed activators, and the acoustic reflex threshold was objectively calculated using a maximum 10 likelihood procedure. To illustrate the principles underlying these new reflex tests, reflex shifts in absorbed sound power and absorbance are presented for data acquired in an adult ear with normal hearing and in two infant ears in the initial and follow-up newborn hearing screening exams, one with normal hearing and the other with a conductive hearing loss. The use of absorbed sound power was helpful in classifying an acoustic reflex shift as present or absent. The resulting reflex tests are in use in a large study of wideband clinical diagnosis and monitoring of middle-ear and cochlear function in infant and adult ears.

Post-Nor'Ida coastal oblique aerial photographs collected from Ocean City, Maryland, to Hatteras, North Carolina, December 4, 2009

USGS Publications Warehouse

Morgan, Karen L. M.; Krohn, M. Dennis; Guy, Kristy K.

2015-01-01

In addition to the photographs, a Google Earth Keyhole Markup Language (KML) file is provided and can be used to view the images by clicking on the marker and then clicking on either the thumbnail or the link above the thumbnail. The KML files were created using the photographic navigation files.

Baseline Coastal Oblique Aerial Photographs Collected from Navarre Beach, Florida, to Breton Island, Louisiana, September 1, 2014

USGS Publications Warehouse

Morgan, Karen L. M.

2015-08-31

In addition to the photographs, a Google Earth Keyhole Markup Language (KML) file is provided and can be used to view the images by clicking on the marker and then clicking on either the thumbnail or the link above the thumbnail. The KML files were created using the photographic navigation files.

The Google Online Marketing Challenge: Real Clients, Real Money, Real Ads and Authentic Learning

ERIC Educational Resources Information Center

Miko, John S.

2014-01-01

Search marketing is the process of utilizing search engines to drive traffic to a Web site through both paid and unpaid efforts. One potential paid component of a search marketing strategy is the use of a pay-per-click (PPC) advertising campaign in which advertisers pay search engine hosts only when their advertisement is clicked. This paper…

If You Say Thee Uh You Are Describing Something Hard: The On-Line Attribution of Disfluency during Reference Comprehension

ERIC Educational Resources Information Center

Arnold, Jennifer E.; Kam, Carla L. Hudson; Tanenhaus, Michael K.

2007-01-01

Eye-tracking and gating experiments examined reference comprehension with fluent (Click on the red. . .) and disfluent (Click on [pause] thee uh red . . .) instructions while listeners viewed displays with 2 familiar (e.g., ice cream cones) and 2 unfamiliar objects (e.g., squiggly shapes). Disfluent instructions made unfamiliar objects more…

Attention: Page has moved

Science.gov Websites

Skip Navigation Links www.nws.noaa.gov NOAA logo - Click to go to the NOAA home page National Weather Service NWS logo - Click to go to the NWS home page Climate Prediction Center Site Map News Organization Search All NWS Search Go CPC Search CPC search Go About Us Our Mission Who We Are Contact Us CPC

The MOC Reflex during Active Listening to Speech

ERIC Educational Resources Information Center

Garinis, Angela C.; Glattke, Theodore; Cone, Barbara K.

2011-01-01

Purpose: The purpose of this study was to test the hypothesis that active listening to speech would increase medial olivocochlear (MOC) efferent activity for the right vs. the left ear. Method: Click-evoked otoacoustic emissions (CEOAEs) were evoked by 60-dB p.e. SPL clicks in 13 normally hearing adults in 4 test conditions for each ear: (a) in…

77 FR 33130 - Special Local Regulation; Kelley's Island Swim, Lake Erie; Kelley's Island, Lakeside, OH

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-05

...), U.S. Department of Transportation, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue... ``USCG-2012-0386'' in the ``Keyword'' box. Click ``Search'' then click on the balloon shape in the... ``Actions'' column. You may also visit the Docket Management Facility in Room W12-140 on the ground floor of...

75 FR 13707 - Safety Zones; Annual Events Requiring Safety Zones in the Captain of the Port Lake Michigan Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-23

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77 FR 16198 - Safety & Security Zones; OPSAIL 2012 Connecticut, Thames River, New London, CT

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-20

... Building Ground Floor, Room W12-140, 1200 New Jersey Avenue SE., Washington, DC 20590-0001. (4) Hand...'' box. Click ``Search'' then click on the balloon shape in the ``Actions'' column. If you submit your... Facility in Room W12-140 on the ground floor of the Department of Transportation West Building, 1200 New...

77 FR 15320 - Special Local Regulations for Marine Events; Temporary Change of Dates for Recurring Marine...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-15

... (M-30), U.S. Department of Transportation, West Building Ground Floor, Room W12-140, 1200 New Jersey...-0109'' in the ``Keyword'' box. Click ``Search'' then click on the balloon shape in the ``Actions...'' column. You may also visit the Docket Management Facility in Room W12-140 on the ground floor of the...

Brick and Click Libraries: Proceedings of an Academic Library Symposium (Northwest Missouri State University, Maryville, Missouri, October 14, 2005)

ERIC Educational Resources Information Center

Ury, Connie Jo., Ed.; Baudino, Frank, Ed.

2005-01-01

These proceedings document the fifth year of the "Brick and Click Libraries Symposium", held annually at Northwest Missouri State University in Maryville, Missouri. Thirty five peer-reviewed papers and abstracts, written by academic librarians, and presented at the symposium are included in this volume. Many of the entries have…

Brick and Click Libraries: Proceedings of an Academic Library Symposium (Maryville, Missouri, October 22, 2004)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Mardis, Lori, Ed.; Park, Sarah G., Ed.; Ury, Connie J., Ed.

2004-01-01

The Brick & Click Libraries Symposium is a one-day conference that focuses on providing library resources and services for students who are either on-campus learners or off-campus learners. It is sponsored by Northwest Missouri State University in order to offer academic librarians a forum for sharing practical information. The subjects and…

De la Perception et de la reproduction des structures rythmiques (On the Perception and Reproduction of Rhythmic Structures)

ERIC Educational Resources Information Center

Minne, Brigitte

1977-01-01

An analysis of rhythm in French. The research project concentrated on two points: the perception of a "click" superimposed on an utterance and the reproduction of the click by a knock simultaneous with the stimulus. Conclusions based on linguistic and non-linguistic aspects are drawn. (Text is in French.) (AMH)

Integrating the Visual Arts Back into the Classroom with Mobile Applications: Teaching beyond the "Click and View" Approach

ERIC Educational Resources Information Center

Katz-Buonincontro, Jen; Foster, Aroutis

2013-01-01

Teachers can use mobile applications to integrate the visual arts back into the classroom, but how? This article generates recommendations for selecting and using well-designed mobile applications in the visual arts beyond a "click and view " approach. Using quantitative content analysis, the results show the extent to which a sample of…

Increasing Seat Belt Use on a College Campus: An Evaluation of Two Prompting Procedures

ERIC Educational Resources Information Center

Clayton, Michael C.; Helms, Bridgett P.

2009-01-01

Seat belt use is an important factor in the prevention of automobile accidents involving injuries and fatalities. The current study used a multielement design to compare the "Click It or Ticket" and "Please Buckle Up--I Care" procedures. Results indicate that the Click It or Ticket prompt resulted in a 20-percentage-point increase in seat belt…

Carpet or Carcel: The Effect of Age of Acquisition and Language Mode on Bilingual Lexical Access

ERIC Educational Resources Information Center

Canseco-Gonzalez, Enriqueta; Brehm, Laurel; Brick, Cameron A.; Brown-Schmidt, Sarah; Fischer, Kara; Wagner, Katie

2010-01-01

Lexical access was examined in English-Spanish bilinguals by monitoring eye fixations on target and lexical competitors as participants followed spoken instructions in English to click on one of the objects presented on a computer (e.g., "Click on the beans"). Within-language lexical competitors had a phoneme onset in English that was shared with…

Search Fermilab Plant Database

Science.gov Websites

Select the characteristics of the plant you want to find below and click the Search button. To see Plants to see all the prairie plants in the database. Click Search All Plants at Fermilab to search for reflects observations at Fermilab. If you need a more sophisticated search, try the Advanced Search. Search

National Centers for Environmental Prediction

Science.gov Websites

Skip Navigation Links www.nws.noaa.gov NOAA logo - Click to go to the NOAA homepage National Weather Service NWS logo - Click to go to the NWS homepage EMC Logo Navigation Bar Left Cap Home News Publications EMC Facebook Contact EMC Webmaster logo image for USA dot gov website. USA dot gov is the U.S

NOAA Photo Library - Sanctuaries

Science.gov Websites

whale tail The word sanctuary evokes images of a sacred place, a refuge from the dangers of the world images contained in the collection. Click on thumbnails to view larger images. ALBUMS Images are arranged by themes. Click on thumbnails to view larger images. Note that not all images are contained in the

NOAA Photo Library - NOAA's Ark/Animals Album

Science.gov Websites

options banner CATALOG View ALL images contained in the collection. Click on thumbnails to view larger images. ALBUMS Images are arranged by themes. Click on thumbnails to view larger images. Note that not all images are contained in the albums - select the above option to view ALL current images. NOAA's

Brick and Click Libraries: Proceedings of an Academic Library Symposium (6th, Maryville, Missouri, November 3, 2006)

ERIC Educational Resources Information Center

Baudino, Frank, Ed.; Ury, Connie Jo, Ed.; Park, Sarah G., Ed.

2006-01-01

These proceedings document the sixth year of the "Brick and Click Libraries Symposium," held annually at Northwest Missouri State University in Maryville, Missouri, on November 3, 2006. Thirty-four peer-reviewed papers and abstracts, written by academic librarians, and presented at the symposium are included in this volume. Many of the…

Evoked potential application to study of echolocation in cetaceans

NASA Astrophysics Data System (ADS)

Supin, Alexander Ya.; Nactigall, Paul E.; Pawloski, Jeffrey; Au, Whitlow W. L.

2002-05-01

The evoked-potential (EP) method is effective in studies of hearing capabilities of cetaceans. However, until now EP studies in cetaceans were performed only in conditions of passive hearing by recording EP to external stimuli. Can this method be applied to study active echolocation in odontocetes? To answer this question, auditory brainstem evoked responses (ABR) were recorded in a false killer whale while the animal echolocated a target within an experiment in which the animal reported the target present or absent. The ABR collection was triggered by echolocation clicks. In these conditions, the recorded ABR pattern contained a duplicate set of waves. A comparison of ABR wave delays recorded during echolocation with those recorded during regular external stimulation has shown that the first set of waves is a response to the emitted click whereas the second one is a response to the echo. Both responses, to the emitted click and to the echo, were of comparable amplitude in spite of the intensity difference of these two sounds of more than 40 dB near the animal's head. This finding indicates some mechanisms releasing responses to echoes from masking by loud emitted clicks. The evoked-potential method may be productive to investigate these mechanisms.

The frequency range of TMJ sounds.

PubMed

Widmalm, S E; Williams, W J; Djurdjanovic, D; McKay, D C

2003-04-01

There are conflicting opinions about the frequency range of temporomandibular joint (TMJ) sounds. Some authors claim that the upper limit is about 650 Hz. The aim was to test the hypothesis that TMJ sounds may contain frequencies well above 650 Hz but that significant amounts of their energy are lost if the vibrations are recorded using contact sensors and/or travel far through the head tissues. Time-frequency distributions of 172 TMJ clickings (three subjects) were compared between recordings with one microphone in the ear canal and a skin contact transducer above the clicking joint and between recordings from two microphones, one in each ear canal. The energy peaks of the clickings recorded with a microphone in the ear canal on the clicking side were often well above 650 Hz and always in a significantly higher area (range 117-1922 Hz, P < 0.05 or lower) than in recordings obtained with contact sensors (range 47-375 Hz) or in microphone recordings from the opposite ear canal (range 141-703 Hz). Future studies are required to establish normative frequency range values of TMJ sounds but need methods also capable of recording the high frequency vibrations.

In situ click chemistry: from small molecule discovery to synthetic antibodies

PubMed Central

Agnew, Heather D.; Lai, Bert; Lee, Su Seong; Lim, Jaehong; Nag, Arundhati; Pitram, Suresh; Rohde, Rosemary; Heath, James R.

2013-01-01

Advances in the fields of proteomics, molecular imaging, and therapeutics are closely linked to the availability of affinity reagents that selectively recognize their biological targets. Here we present a review of Iterative Peptide In Situ Click Chemistry (IPISC), a novel screening technology for designing peptide multiligands with high affinity and specificity. This technology builds upon in situ click chemistry, a kinetic target-guided synthesis approach where the protein target catalyzes the conjugation of two small molecules, typically through the azide–alkyne Huisgen cycloaddition. Integrating this methodology with solid phase peptide libraries enables the assembly of linear and branched peptide multiligands we refer to as Protein Catalyzed Capture Agents (PCC Agents). The resulting structures can be thought of as analogous to the antigen recognition site of antibodies and serve as antibody replacements in biochemical and cell-based applications. In this review, we discuss the recent progress in ligand design through IPISC and related approaches, focusing on the improvements in affinity and specificity as multiligands are assembled by target-catalyzed peptide conjugation. We compare the IPISC process to small molecule in situ click chemistry with particular emphasis on the advantages and technical challenges of constructing antibody-like PCC Agents. PMID:22836343

Clicks, whistles and pulses: Passive and active signal use in dolphin communication

NASA Astrophysics Data System (ADS)

Herzing, Denise L.

2014-12-01

The search for signals out of noise is a problem not only with radio signals from the sky but in the study of animal communication. Dolphins use multiple modalities to communicate including body postures, touch, vision, and most elaborately sound. Like SETI radio signal searches, dolphin sound analysis includes the detection, recognition, analysis, and interpretation of signals. Dolphins use both passive listening and active production to communicate. Dolphins use three main types of acoustic signals: frequency modulated whistles (narrowband with harmonics), echolocation (broadband clicks) and burst pulsed sounds (packets of closely spaced broadband clicks). Dolphin sound analysis has focused on frequency-modulated whistles, yet the most commonly used signals are burst-pulsed sounds which, due to their graded and overlapping nature and bimodal inter-click interval (ICI) rates are hard to categorize. We will look at: 1) the mechanism of sound production and categories of sound types, 2) sound analysis techniques and information content, and 3) examples of lessons learned in the study of dolphin acoustics. The goal of this paper is to provide perspective on how animal communication studies might provide insight to both passive and active SETI in the larger context of searching for life signatures.

All in the family: matrimonial mitral valve clicks.

PubMed

Desser, K B; Bokhari, S I; Benchimol, A; Romney, D

1981-05-01

Mitral valve clicks with or without late systolic murmurs were detected in genetically unrelated marital partners of 5 families. The first family represented 2 successive nonconsanguineous marital unions with 3 generations of mitral valve clicks. The second family included 1 natural and 2 adopted children with clinical and echographic evidence of mitral valve prolapse. The third family was comprised of asymptomatic parents, both with nonejection clicks and mitral valve prolapse, whose daughter presented 3 years previously with syncope, palpitations, and combined mitral and tricuspid valve prolapse. The fourth family had 3 members with auscultatory and ultrasonic manifestations of billowing mitral valve, whereas the fourth member had "silent mitral valve prolapse." The fifth family represented a mother with auscultatory and echographic evidence of mitral valve prolapse; her 14-year-old daughter had both mitral and tricuspid valve prolapse, whereas the son had a bicuspid aortic valve. Both children were products of a prior marriage, and her husband has symptomatic mitral valve prolapse. We conclude that matrimonial mitral valve prolapse probably reflects the purported (6--10%) prevalence of this disorder in the general population. The consequences of such marital union on progeny is currently unclear and warrants future investigation.

Biosonar performance of foraging Blainvilles beaked whales (Mesoplodon densirostris)

NASA Astrophysics Data System (ADS)

Madsen, Peter T.; Johnson, Mark; Tyack, Peter L.; Aguilar de Soto, Natacha; Zimmer, Walter M. X.

2004-05-01

Echolocating animals like bats and toothed whales navigate and locate food by means of echoes from sounds transmitted by the animals themselves. Toothed whale echolocation has been studied intensively in captivity, but little information exists on how echolocation is used by wild animals for orientation and prey location. To expand on this issue, a noninvasive, acoustic Dtag (96-kHz sampling, 16-bit resolution) was deployed on two Blainvilles beaked whales. The tagged whales only clicked at depths below 200 m during deep foraging dives. The echolocation clicks are directional, 250-ms transients with peak energy in the 30-40-kHz band. Echoes from the seafloor and from prey items were recorded. The regular click rate is not adjusted to the decreasing echo delay from incoming prey until the target is within an approximate body length of the whale after which the click rate is increased rapidly akin to the buzz phase of echolocating bats. This suggests that the whales use different sonar strategies for operating in near versus far field modes. Changes in received echo intensities from prey targets during approaches are compared to the active gain control in the receiving system of bats and in the transmitting system of dolphins.

Organogold oligomers: Exploiting iClick and aurophilic cluster formation to prepare solution stable Au 4 repeating units

DOE PAGES

Yang, Xi; Wang, Shanshan; Ghiviriga, Ion; ...

2015-05-19

A novel synthetic method to create gold based metallo–oligomers/polymers via the combination of inorganic click (iClick) with intermolecular aurophilic interactions is demonstrated. Complexes [PEt 3Au] 4(μ-N 3C 2C 6H 5) (1) and [PPhMe 2Au] 43C 2C 6H 5) (2) and {[PEt 3Au] 4[(μ-N 3C 2) 2-9,9-dihexyl-9H-fluorene]} n (8) have been synthesized via iClick. The tetranuclear structures of 1 and 2, induced by aurophilic bonding, are confirmed in the solid state through single crystal X-ray diffraction experiments and in solution via variable temperature NMR spectroscopy. The extended 1D structure of 8 is constructed by aurophilic induced self-assembly. 1H DOSY NMR analysismore » reveals that the aurophilic bonds in 1, 2, and 8 are retained in the solution phase. The degree of polymerization within complex 8 is temperature and concentration dependent, as determined by 1H DOSY NMR. The complex 8 is a rare example of a solution stable higher ordered structure linked by aurophilic interactions.« less

Click-crosslinkable and photodegradable gelatin hydrogels for cytocompatible optical cell manipulation in natural environment

PubMed Central

Tamura, Masato; Yanagawa, Fumiki; Sugiura, Shinji; Takagi, Toshiyuki; Sumaru, Kimio; Kanamori, Toshiyuki

2015-01-01

This paper describes the generation of “click-crosslinkable“ and “photodegaradable“ gelatin hydrogels from the reaction between dibenzocycloctyl-terminated photoclevable tetra-arm polyethylene glycol and azide-modified gelatin. The hydrogels were formed in 30 min through the click-crosslinking reaction. The micropatterned features in the hydrogels were created by micropatterned light irradiation; the minimum resolution of micropatterning was 10-μm widths for line patterns and 20-μm diameters for circle patterns. Cells were successfully encapsulated in the hydrogels without any loss of viability across a wide concentration range of crosslinker. In contrast, an activated-ester-type photocleavable crosslinker, which we previously used to prepare photodegradable gelatin hydrogels, induced a decrease in cell viability at crosslinker concentrations greater than 1.8 mM. We also observed morphology alteration and better growth of cancer cells in the click-crosslinked photodegradable gelatin hydrogels that included matrigel than in the absence of matrigel. We also demonstrated micropatterning of the hydrogels encapsulating cells and optical cell separation. Both of the cells that remained in the non-irradiated area and the cells collected from the irradiated area maintained their viability. PMID:26450015

Beaked whales echolocate on prey.

PubMed Central

Johnson, Mark; Madsen, Peter T; Zimmer, Walter M X; de Soto, Natacha Aguilar; Tyack, Peter L

2004-01-01

Beaked whales (Cetacea: Ziphiidea) of the genera Ziphius and Mesoplodon are so difficult to study that they are mostly known from strandings. How these elusive toothed whales use and react to sound is of concern because they mass strand during naval sonar exercises. A new non-invasive acoustic ording tag was attached to four beaked whales(two Mesoplodon densirostris and two Ziphius cavirostris) and recorded high-frequency clicks during deep dives. The tagged whales only clicked at depths below 200 m, down to a maximum depth of 1267 m. Both species produced a large number of short, directional, ultrasonic clicks with significant energy below 20 kHz. The tags recorded echoes from prey items; to our knowledge, a first for any animal echolocating in the wild. As far as we are aware, these echoes provide the first direct evidence on how free-ranging toothed whales use echolocation in foraging. The strength of these echoes suggests that the source level of Mesoplodon clicks is in the range of 200-220 dB re 1 microPa at 1 m.This paper presents conclusive data on the normal vocalizations of these beaked whale species, which may enable acoustic monitoring to mitigate exposure to sounds intense enough to harm them. PMID:15801582

The Complex Dynamics of Sponsored Search Markets

NASA Astrophysics Data System (ADS)

Robu, Valentin; La Poutré, Han; Bohte, Sander

This paper provides a comprehensive study of the structure and dynamics of online advertising markets, mostly based on techniques from the emergent discipline of complex systems analysis. First, we look at how the display rank of a URL link influences its click frequency, for both sponsored search and organic search. Second, we study the market structure that emerges from these queries, especially the market share distribution of different advertisers. We show that the sponsored search market is highly concentrated, with less than 5% of all advertisers receiving over 2/3 of the clicks in the market. Furthermore, we show that both the number of ad impressions and the number of clicks follow power law distributions of approximately the same coefficient. However, we find this result does not hold when studying the same distribution of clicks per rank position, which shows considerable variance, most likely due to the way advertisers divide their budget on different keywords. Finally, we turn our attention to how such sponsored search data could be used to provide decision support tools for bidding for combinations of keywords. We provide a method to visualize keywords of interest in graphical form, as well as a method to partition these graphs to obtain desirable subsets of search terms.

Negative Prospective Memory in Alzheimer's Disease: "Do Not Perform That Action".

PubMed

El Haj, Mohamad; Coello, Yann; Kapogiannis, Dimitrios; Gallouj, Karim; Antoine, Pascal

2018-01-01

Relatively to "standard" prospective memory, i.e., remembering to perform a future action, little is known about negative prospective memory, i.e., remembering not to perform a future action. This study investigated the latter ability in Alzheimer's disease (AD). AD participants and healthy older adults were asked to click on the keyboard or not to click on it when a cue word was encountered. Results showed more omissions (i.e., forgetting to click the keyboard when the instruction was to do so) in AD participants than in healthy older adults, suggesting a prospective memory deficit. Interestingly, more commissions (i.e., clicking the keyboard when the instruction was not to do so) were also observed in AD participants than in healthy older adults. Similar levels of commissions and omissions were observed in AD participants and in healthy older adults. Also, commissions and omissions were correlated with performance on an inhibition assessment task. Our findings reveal that AD is characterized by not only difficulty in the retrieval of recent information, but also difficulty to inhibit no-longer appropriate stimulus-response associations previously learned, suggesting a specific deficit of negative prospective memory in AD.

Protein addressing on patterned microchip by coupling chitosan electrodeposition and 'electro-click' chemistry.

PubMed

Shi, Xiao-Wen; Qiu, Ling; Nie, Zhen; Xiao, Ling; Payne, Gregory F; Du, Yumin

2013-12-01

Many applications in proteomics and lab-on-chip analysis require methods that guide proteins to assemble at surfaces with high spatial and temporal control. Electrical inputs are particularly convenient to control, and there has been considerable effort to discover simple and generic mechanisms that allow electrical inputs to trigger protein assembly on-demand. Here, we report the electroaddressing of a protein to a patterned surface by coupling two generic electroaddressing mechanisms. First, we electrodeposit the stimuli-responsive film-forming aminopolysaccharide chitosan to form a hydrogel matrix at the electrode surface. After deposition, the matrix is chemically functionalized with alkyne groups. Second, we ''electro-click' an azide-tagged protein to the functionalized matrix using electrical signals to trigger conjugation by Huisgen 1,3-dipolar cycloadditions. Specifically, a cathodic potential is applied to the matrix-coated electrode to reduce Cu(II) to Cu(I) which is required for the click reaction. Using fluorescently-labeled bovine serum albumin as our model, we demonstrate that protein conjugation can be controlled spatially and temporally. We anticipate that the coupling of polysaccharide electrodeposition and electro-click chemistry will provide a simple and generic approach to electroaddress proteins within compatible hydrogel matrices.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gonzalez, Silvia A.; Paladino, Monica G.; Affranchino, Jose L., E-mail: jose.affranchino@comunidad.ub.edu.ar

The feline immunodeficiency virus (FIV) envelope glycoprotein (Env) possesses a short cytoplasmic domain of 53 amino acids containing four highly conserved cysteines at Env positions 804, 811, 815 and 848. Since palmitoylation of transmembrane proteins occurs at or near the membrane anchor, we investigated whether cysteines 804, 811 and 815 are acylated and analyzed the relevance of these residues for Env functions. Replacement of cysteines 804, 811 and 815 individually or in combination by serine residues resulted in Env glycoproteins that were efficiently expressed and processed. However, mutations C804S and C811S reduced Env fusogenicity by 93% and 84%, respectively, comparedmore » with wild-type Env. By contrast, mutant C815S exhibited a fusogenic capacity representing 50% of the wild-type value. Remarkably, the double mutation C804S/C811S abrogated both Env fusion activity and Env incorporation into virions. Finally, by means of Click chemistry assays we demonstrated that the four FIV Env cytoplasmic cysteines are palmitoylated.« less

Change in echolocation signals with hearing loss in a false killer whale (Pseudorca crassidens).

PubMed

Kloepper, Laura N; Nachtigall, Paul E; Breese, Marlee

2010-10-01

The echolocation signals of a false killer whale (Pseudorca crassidens) were collected during a wall thickness discrimination task and compared to clicks recorded during an identical experiment in 1992. During the sixteen year time period, the subject demonstrated a loss of high frequency hearing of about 70 kHz. Clicks between the two experiments were compared to investigate the effect of hearing loss on echolocation signals. There was a significant reduction in the peak frequency, center frequency and source level of clicks between the two time periods. Additionally, the subject currently produces more signals with low frequency peaks and fewer signals with high frequency peaks than she did in 1992. These results indicate the subject changed its echolocation signals to match its range of best hearing.

Carbohydrate CuAAC click chemistry for therapy and diagnosis.

PubMed

He, Xiao-Peng; Zeng, Ya-Li; Zang, Yi; Li, Jia; Field, Robert A; Chen, Guo-Rong

2016-06-24

Carbohydrates are important as signaling molecules and for cellular recognition events, therefore offering scope for the development of carbohydrate-mimetic diagnostics and drug candidates. As a consequence, the construction of carbohydrate-based bioactive compounds and sensors has become an active research area. While the advent of click chemistry has greatly accelerated the progress of medicinal chemistry and chemical biology, recent literature has seen an extensive use of such approaches to construct functionally diverse carbohydrate derivatives. Here we summarize some of the progress, covering the period 2010 to mid-2015, in Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition CuAAC "click chemistry" of carbohydrate derivatives, in the context of potential therapeutic and diagnostic tool development. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Flexible Piezoelectric Energy Harvesting from Mouse Click Motions

PubMed Central

Cha, Youngsu; Hong, Jin; Lee, Jaemin; Park, Jung-Min; Kim, Keehoon

2016-01-01

In this paper, we study energy harvesting from the mouse click motions of a robot finger and a human index finger using a piezoelectric material. The feasibility of energy harvesting from mouse click motions is experimentally and theoretically assessed. The fingers wear a glove with a pocket for including the piezoelectric material. We model the energy harvesting system through the inverse kinematic framework of parallel joints in a finger and the electromechanical coupling equations of the piezoelectric material. The model is validated through energy harvesting experiments in the robot and human fingers with the systematically varying load resistance. We find that energy harvesting is maximized at the matched load resistance to the impedance of the piezoelectric material, and the harvested energy level is tens of nJ. PMID:27399705

Modular "Click" Preparation of Bifunctional Polymeric Heterometallic Catalysts.

PubMed

Wang, Wenlong; Zhao, Liyuan; Lv, Hui; Zhang, Guodong; Xia, Chungu; Hahn, F Ekkehardt; Li, Fuwei

2016-06-27

Heterobimetallic molecular complexes or strictly alternating metallated polymers are obtained by a click reaction between mononuclear metal complexes (secondary building units, SBUs) bearing NHCs functionalized with either p-azidophenyl or p-ethynylphenyl wingtips. With a copper-NHC complex as SBU the formation of molecular or polymeric compounds did not require any additives as the copper complex catalyzes the click reaction. Transmetallation from heterobimetallic Cu/Ag derivatives to Cu/Pd derivatives was achieved. The linker between the SBUs (flexible or rigid) influences the catalytic activity of the heterobimetallic compounds. The polymer with alternating copper-NHC and silver-NHC units and a flexible methylene-triazole bridge between them shows the highest activity in the catalytic alkynylation of trifluoromethyl ketones to give fluorinated propargylic alcohols. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Facile purification and click labeling with 2-[ 18F]fluoroethyl azide using solid phase extraction cartridges

DOE PAGES

Zhou, Dong; Chu, Wenhua; Peng, Xin; ...

2014-11-04

In this paper, a facile method was developed to purify 2-[ 18F]fluoroethyl azide ([ 18F]FEA) using a C18 cartridge and an Oasis® HLB cartridge in series, in which [18F]FEA was exclusively trapped on the HLB cartridge. [ 18F]FEA can be eluted for reactions in solution; alternatively click labeling can be carried out on the HLB cartridge itself by loading an alkyne substrate and copper (I) catalyst dissolved in DMF onto the cartridge. Finally, this solid phase extraction methodology for purification and click labeling with [ 18F]FEA, either in solution or on the cartridge, is safe, simple, reproducible in high yield,more » and compatible with automated synthesis of 18F-labeled PET tracers.« less

Transformer-4 version 2.0.1, a free multi-platform software to quickly reformat genotype matrices of any marker type, and archive them in the Demiurge information system.

PubMed

Caujapé-Castells, Juli; Sabbagh, Izzat; Castellano, José J; Ramos, Rafael; Henríquez, Víctor; Quintana, Francisco M; Medina, Dailos A; Toledo, Javier; Ramírez, Fernando; Rodríguez, Juan F

2013-05-01

Transformer-4 version 2.0.1 (T4) is a multi-platform freeware programmed in java that can transform a genotype matrix in Excel or XML format into the input formats of one or several of the most commonly used population genetic software, for any possible combination of the populations that the matrix contains. T4 also allows the users to (i) draw allozyme gel interpretations for any number of diploid individuals, and then generate a genotype matrix ready to be used by T4; and (ii) produce basic reports about the data in the matrices. Furthermore, T4 is the only way to optionally submit 'genetic diversity digests' for publication in the Demiurge online information system (http://www.demiurge-project.org). Each such digest undergoes peer-review, and it consists of a geo-referenced data matrix in the tfm4 format plus any ancillary document or hyperlink that the digest authors see fit to include. The complementarity between T4 and Demiurge facilitates a free, safe, permanent, and standardized data archival and analysis system for researchers, and may also be a convenient resource for scientific journals, public administrations, or higher educators. T4 and its converters are freely available (at, respectively, http://www.demiurge-project.org/download_t4 and http://www.demiurge-project.org/converterstore) upon registration in the Demiurge information system (http://demiurge-project.org/register). Users have to click on the link provided on an account validation email, and accept Demiurge's terms of use (see http://www.demiurge-project.org/termsofuse). A thorough user's guide is available within T4. A 3-min promotional video about T4 and Demiurge can be seen at http://vimeo.com/29828406. © 2013 Blackwell Publishing Ltd.

An Expert System for the Evaluation of Cost Models

DTIC Science & Technology

1990-09-01

contrast to the condition of equal error variance, called homoscedasticity. (Reference: Applied Linear Regression Models by John Neter - page 423...normal. (Reference: Applied Linear Regression Models by John Neter - page 125) Click Here to continue -> Autocorrelation Click Here for the index - Index...over time. Error terms correlated over time are said to be autocorrelated or serially correlated. (REFERENCE: Applied Linear Regression Models by John

Baseline coastal oblique aerial photographs collected from the Virginia/North Carolina border to Montauk Point, New York, October 5-6, 2014

USGS Publications Warehouse

Morgan, Karen L. M.

2015-10-02

In addition to the photographs, a Google Earth Keyhole Markup Language (KML) file is provided and can be used to view the images by clicking on the marker and then clicking on either the thumbnail or the link above the thumbnail. The KML files were created using the photographic navigation files.

Climate Prediction Center - Global Tropical Hazards Assessment

Science.gov Websites

Skip Navigation Links www.nws.noaa.gov NOAA logo - Click to go to the NOAA home page National Weather Service NWS logo - Click to go to the NWS home page Climate Prediction Center Home Site Map News Organization Search Go Search the CPC Go Climate Outlooks Climate & Weather Link El Niño/La Niña MJO

Climate Prediction Center - Monitoring & Data: La Niña Seasonal Maps and

Science.gov Websites

Statistics Skip Navigation Links www.nws.noaa.gov NOAA logo - Click to go to the NOAA home page National Weather Service NWS logo - Click to go to the NWS home page Climate Prediction Center Home Site Map News Organization Search Go Search the CPC Go About Us Our Mission Who We Are Contact Us

Brick and Click Libraries: Changes and Challenges. Proceedings of a Regional Academic Library Symposium (Maryville, Missouri, October 18, 2002).

ERIC Educational Resources Information Center

Ury, Connie Jo, Ed.; Wainscott, Vicki, Ed.

This proceedings of the Brick and Click Libraries Symposium contains the following papers: (1) "Electronic Journals: Vendor Solutions to Access Issues" (Nona Barton); (2) "Tips on Funding" (Janice Borey); (3) "Why Are So Many Web Pages Still So Hard To Use?" (Jerry R. Brown); (4) "The Secrets of Full-Text Databases: The Overlap between a Same…

SSC San Diego Biennial Review 2003. Command and Control

DTIC Science & Technology

2003-01-01

systems. IMAT systems use scientific visualizations, three- dimensional graphics, and animations to illustrate com- plex physical interactions in mission...Again, interactive animations are used to explain underlying concepts. For exam- ple, for principles of beamforming using a phased array, a three...solve complex problems. Experts type natural language text, use mouse clicks to provide hints for explanation generation, and use mouse clicks to

76 FR 5809 - Notice of Public Information Collection(s) Being Submitted for Review and Approval to the Office...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-02

... Under Review'', (3) click on the downward-pointing arrow in the ``Select Agency'' box below the... presented in the ``Select Agency'' box, (5) click the ``Submit'' button to the right of the ``Select Agency'' box, and (6) when the list of FCC ICRs currently under review appears, look for the title of this ICR...

77 FR 42251 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Reef Fish Fishery of the Gulf of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-18

....gov , enter ``NOAA-NMFS-2011-0025'' in the search field and click on ``search''. After you locate the proposed rule, click the ``Submit a Comment'' link in that row. This will display the comment web form. You...), however, the current burden estimate (20 minutes per applicant) to complete the application form would not...

77 FR 277 - Sabine Pass Liquefaction, LLC and Sabine Pass LNG, L.P; Notice of Availability of the...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-04

... Web site ( www.ferc.gov ) using the eLibrary link. A limited number of copies of the EA are available..., or on the FERC Web site ( www.ferc.gov ) using the eLibrary link. Click on the eLibrary link, click... systems, induced draft air coolers, fire and gas detection and safety systems, control systems, and...

75 FR 20802 - Safety Zone; New York Air Show at Jones Beach State Park, Atlantic Ocean off of Jones Beach...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-21

... Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE., Washington, DC 20590-0001. Hand Delivery... the ``Keyword'' box. Click ``Search'' then click on the balloon shape in the ``Actions'' column. If... also visit the Docket Management Facility in Room W12-140 on the ground floor of the Department of...

How Users Take Advantage of Different Forms of Interactivity on Online News Sites: Clicking, E-Mailing, and Commenting

ERIC Educational Resources Information Center

Boczkowski, Pablo J.; Mitchelstein, Eugenia

2012-01-01

This study examines the uptake of multiple interactive features on news sites. It looks at the thematic composition of the most clicked, most e-mailed, and most commented stories during periods of heightened and routine political activity. Results show that (a) during the former period, the most commented stories were more likely to be focused on…

Teaching Skills to Use a Computer Mouse in Preschoolers with Developmental Disabilities: Shaping Moving a Mouse and Eye-Hand Coordination

ERIC Educational Resources Information Center

Shimizu, Hirofumi; Yoon, Soyoung; McDonough, Christopher S.

2010-01-01

We taught seven preschoolers with developmental disabilities to point-and-click with a computer mouse. The computer-based training program consisted of three parts, based on a task analysis of the behavioral prerequisites to point-and-click. Training 1 was designed to shape moving the mouse. Training 2 was designed to build eye-hand coordination…

Brick and Click Libraries: Proceedings of a Regional Academic Library Symposium (Missouri State University, Maryville, Missouri, October 10, 2003)

ERIC Educational Resources Information Center

Ury, Connie Jo, Ed.; Baudino, Frank, Ed.

2003-01-01

The 2003 Brick and Click is a one-day conference that focuses on providing library resources and services for students who are either on-campus learners or off-campus learners. The conference theme was "The Shape of Tomorrow". It is sponsored by the Northwest Missouri State University in order to offer academic librarians a forum for…

Functionalization of multilayer fullerenes (carbon nano-onions) using diazonium compounds and "click" chemistry.

PubMed

Flavin, Kevin; Chaur, Manuel N; Echegoyen, Luis; Giordani, Silvia

2010-02-19

A novel versatile approach for the functionalization of multilayer fullerenes (carbon nano-onions) has been developed, which involves the facile introduction of a variety of simple functionalities onto their surface by treatment with in situ generated diazonium compounds. This approach is complemented by use of "click" chemistry which was used for the covalent introduction of more complex porphyrin molecules.

Facile and Stabile Linkages through Tyrosine: Bioconjugation Strategies with the Tyrosine-Click Reaction

PubMed Central

Ban, Hitoshi; Nagano, Masanobu; Gavrilyuk, Julia; Hakamata, Wataru; Inokuma, Tsubasa; Barbas, Carlos F.

2013-01-01

The scope, chemoselectivity, and utility of the click-like tyrosine labeling reaction with 4-phenyl-3H-1,2,4-triazoline-3,5(4H)-diones (PTADs) is reported. To study the utility and chemoselectivity of PTAD derivatives in peptide and protein chemistry, we synthesized PTAD derivatives possessing azide, alkyne, and ketone groups and studied their reactions with amino acid derivatives and peptides of increasing complexity. With proteins we studied the compatibility of the tyrosine click reaction with cysteine and lysine-targeted labeling approaches and demonstrate that chemoselective tri-functionalization of proteins is readily achieved. In particular cases, we noted PTAD decomposition resulted in formation of a putative isocyanate by-product that was promiscuous in labeling. This side reaction product, however, was readily scavenged by the addition of a small amount of 2-amino-2-hydroxymethyl-propane-1,3-diol (Tris) to the reaction medium. To study the potential of the tyrosine click reaction to introduce poly(ethylene) glycol chains onto proteins (PEGylation), we demonstrate that this novel reagent provides for the selective PEGylation of chymotrypsinogen whereas traditional succinimide-based PEGylation targeting lysine residues provided a more diverse range of PEGylated products. Finally, we applied the tyrosine click reaction to create a novel antibody drug conjugate. For this purpose, we synthesized a PTAD derivative linked to the HIV entry inhibitor aplaviroc. Labeling of the antibody trastuzumab with this reagent provided a labeled antibody conjugate that demonstrated potent HIV-1 neutralization activity demonstrating the potential of this reaction in creating protein conjugates with small molecules. The tyrosine click linkage demonstrated stability to extremes of pH, temperature and exposure to human blood plasma indicating that this linkage is significantly more robust than maleimide-type linkages that are commonly employed in bioconjugations. These studies support the broad utility of this reaction in the chemoselective modification of small molecules, peptides, and proteins under mild aqueous conditions over a broad pH range using a wide variety of biologically acceptable buffers such as phosphate buffered saline (PBS) and 2-amino-2-hydroxymethyl-propane-1,3-diol (Tris) buffers as well as others and mixed buffered compositions. PMID:23534985

Are chirps better than clicks and tonebursts for evoking middle latency responses?

PubMed

Atcherson, Samuel R; Moore, Page C

2014-06-01

The middle latency response (MLR) is considered a valid clinical tool for assessing the integrity of cortical and subcortical structures. Several investigators have demonstrated that a rising frequency chirp stimulus is capable of eliciting not only larger wave V amplitudes but larger MLR components as well. However, the chirp has never been specifically examined in a hemispheric electrode montage setup that is typical for neurodiagnostic application and site-of-lesion testing. The purpose of this study was to examine the effect of chirp, click, and toneburst stimuli on MLR waveform peak latency and peak-to-peak amplitude in a hemispheric electrode montage setup. This study used a repeated-measures design. A total of 10 young adult participants (3 males, 7 females) with normal hearing were recruited and had negative histories of audiologic, otologic, and neurologic involvement, and no reported language or learning difficulties. MLR latencies (Na, Pa, Nb, and Pb) and peak-to-peak amplitudes (Na-Pa, Pa-Nb, and Nb-Pb) were measured for all conditions and were statistically evaluated for left hemisphere-right ear (C3-A2) and right hemisphere-left ear (C4-A1) recordings. Statistical analyses revealed no significant difference between C3-A2 and C4-A1 peak-to-peak amplitudes; therefore, data were collapsed. Stimulus comparisons revealed that Na evoked by tonebursts were statistically prolonged compared with both chirp and click, and that both Na-Pa and Pa-Nb peak-to-peak amplitudes were statistically larger for chirps compared with both clicks and tonebursts, and for clicks compared with tonebursts. The results of this study support the hypothesis that a chirp would offer a clinical advantage to the click and toneburst in overall peak-to-peak amplitude. As expected, normal-hearing participants did not exhibit hemispheric differences when comparing C3-A2 and C4-A1 peak-to-peak amplitudes demonstrating symmetric auditory brain function. However, chirp-evoked MLRs will require further study to determine its usefulness in clinical practice. American Academy of Audiology.

General Dialdehyde Click Chemistry for Amine Bioconjugation.

PubMed

Elahipanah, Sina; O'Brien, Paul J; Rogozhnikov, Dmitry; Yousaf, Muhammad N

2017-05-17

The development of methods for conjugating a range of molecules to primary amine functional groups has revolutionized the fields of chemistry, biology, and material science. The primary amine is a key functional group and one of the most important nucleophiles and bases used in all of synthetic chemistry. Therefore, tremendous interest in the synthesis of molecules containing primary amines and strategies to devise chemical reactions to react with primary amines has been at the core of chemical research. In particular, primary amines are a ubiquitous functional group found in biological systems as free amino acids, as key side chain lysines in proteins, and in signaling molecules and metabolites and are also present in many natural product classes. Due to its abundance, the primary amine is the most convenient functional group handle in molecules for ligation to other molecules for a broad range of applications that impact all scientific fields. Because of the primary amine's central importance in synthetic chemistry, acid-base chemistry, redox chemistry, and biology, many methods have been developed to efficiently react with primary amines, including activated carboxylic acids, isothiocyanates, Michael addition type systems, and reaction with ketones or aldehydes followed by in situ reductive amination. Herein, we introduce a new traceless, high-yield, fast click-chemistry method based on the rapid and efficient trapping of amine groups via a functionalized dialdehyde group. The click reaction occurs in mild conditions in organic solvents or aqueous media and proceeds in high yield, and the starting dialdehyde reagent and resulting dialdehyde click conjugates are stable. Moreover, no catalyst or dialdehyde-activating group is required, and the only byproduct is water. The initial dialdehyde and the resulting conjugate are both straightforward to characterize, and the reaction proceeds with high atom economy. To demonstrate the broad scope of this new click-conjugation strategy, we designed a straightforward scheme to synthesize a suite of dialdehyde reagents. The dialdehyde molecules were used for applications in cell-surface engineering and for tailoring surfaces for material science applications. We anticipate the broad utility of the general dialdehyde click chemistry to primary amines in all areas of chemical research, ranging from polymers and bioconjugation to material science and nanoscience.

Accuracy of Geographically Targeted Internet Advertisements on Google Adwords for Recruitment in a Randomized Trial

PubMed Central

Goldsmith, Lesley; Williams, Christopher J; Kamel Boulos, Maged N

2012-01-01

Background Google AdWords are increasingly used to recruit people into research studies and clinical services. They offer the potential to recruit from targeted control areas in cluster randomized controlled trials (RCTs), but little is known about the feasibility of accurately targeting ads by location and comparing with control areas. Objective To examine the accuracy and contamination of control areas by a location-targeted online intervention using Google AdWords in a pilot cluster RCT. Methods Based on previous use of online cognitive behavioral therapy for depression and population size, we purposively selected 16 of the 121 British postcode areas and randomized them to three intervention and one (do-nothing) control arms. Two intervention arms included use of location-targeted AdWords, and we compared these with the do-nothing control arm. We did not raise the visibility of our research website to normal Web searches. Users who clicked on the ad were directed to our project website, which collected the computer Internet protocol (IP) address, date, and time. Visitors were asked for their postcode area and to complete the Patient Health Questionnaire (depression). They were then offered links to several online depression resources. Google Analytics largely uses IP methods to estimate location, but AdWords uses additional information. We compared locations assessed by (1) Analytics, and (2) as self-identified by users. Results Ads were shown 300,523 times with 4207 click-throughs. There were few site visits except through AdWord click-throughs. Both methods of location assessment agreed there was little contamination of control areas. According to Analytics, 69.75% (2617/3752) of participants were in intervention areas, only 0% (8/3752) in control areas, but 30.04% (1127/3752) in other areas. However, according to user-stated postcodes, only 20.7% (463/2237) were in intervention areas, 1% (22/2236) in control areas, but 78.31% (1751/2236) in other areas. Both location assessments suggested most leakage from the intervention arms was to nearby postcode areas. Analytics data differed from postcodes reported by participants. Analysis of a subset of 200/2236 records over 10 days comparing IP-estimated location with stated postcode suggested that Google AdWords targeted correctly in just half the cases. Analytics agreed with our assessment that, overall, one-third were wrongly targeted by AdWords. There appeared little evidence that people who bothered to give their postcode did not answer truthfully. Conclusions Although there is likely to be substantial leakage from the targeted areas, if intervention and control areas are a sufficient distance apart, it is feasible to conduct a cluster RCT using online ads to target British postcode areas without significant contamination. Trial Registration Clinicaltrials.gov NCT01469689; http://clinicaltrials.gov/ct2/show/NCT01469689 (Archived by WebCite at http://www.webcitation.org/681iro5OU) PMID:22718043

Accuracy of geographically targeted internet advertisements on Google AdWords for recruitment in a randomized trial.

PubMed

Jones, Ray B; Goldsmith, Lesley; Williams, Christopher J; Kamel Boulos, Maged N

2012-06-20

Google AdWords are increasingly used to recruit people into research studies and clinical services. They offer the potential to recruit from targeted control areas in cluster randomized controlled trials (RCTs), but little is known about the feasibility of accurately targeting ads by location and comparing with control areas. To examine the accuracy and contamination of control areas by a location-targeted online intervention using Google AdWords in a pilot cluster RCT. Based on previous use of online cognitive behavioral therapy for depression and population size, we purposively selected 16 of the 121 British postcode areas and randomized them to three intervention and one (do-nothing) control arms. Two intervention arms included use of location-targeted AdWords, and we compared these with the do-nothing control arm. We did not raise the visibility of our research website to normal Web searches. Users who clicked on the ad were directed to our project website, which collected the computer Internet protocol (IP) address, date, and time. Visitors were asked for their postcode area and to complete the Patient Health Questionnaire (depression). They were then offered links to several online depression resources. Google Analytics largely uses IP methods to estimate location, but AdWords uses additional information. We compared locations assessed by (1) Analytics, and (2) as self-identified by users. Ads were shown 300,523 times with 4207 click-throughs. There were few site visits except through AdWord click-throughs. Both methods of location assessment agreed there was little contamination of control areas. According to Analytics, 69.75% (2617/3752) of participants were in intervention areas, only 0% (8/3752) in control areas, but 30.04% (1127/3752) in other areas. However, according to user-stated postcodes, only 20.7% (463/2237) were in intervention areas, 1% (22/2236) in control areas, but 78.31% (1751/2236) in other areas. Both location assessments suggested most leakage from the intervention arms was to nearby postcode areas. Analytics data differed from postcodes reported by participants. Analysis of a subset of 200/2236 records over 10 days comparing IP-estimated location with stated postcode suggested that Google AdWords targeted correctly in just half the cases. Analytics agreed with our assessment that, overall, one-third were wrongly targeted by AdWords. There appeared little evidence that people who bothered to give their postcode did not answer truthfully. Although there is likely to be substantial leakage from the targeted areas, if intervention and control areas are a sufficient distance apart, it is feasible to conduct a cluster RCT using online ads to target British postcode areas without significant contamination. Clinicaltrials.gov NCT01469689; http://clinicaltrials.gov/ct2/show/NCT01469689 (Archived by WebCite at http://www.webcitation.org/681iro5OU).

On doing two things at once: dolphin brain and nose coordinate sonar clicks, buzzes and emotional squeals with social sounds during fish capture.

PubMed

Ridgway, Sam; Samuelson Dibble, Dianna; Van Alstyne, Kaitlin; Price, DruAnn

2015-12-01

Dolphins fishing alone in open waters may whistle without interrupting their sonar clicks as they find and eat or reject fish. Our study is the first to match sound and video from the dolphin with sound and video from near the fish. During search and capture of fish, free-swimming dolphins carried cameras to record video and sound. A hydrophone in the far field near the fish also recorded sound. From these two perspectives, we studied the time course of dolphin sound production during fish capture. Our observations identify the instant of fish capture. There are three consistent acoustic phases: sonar clicks locate the fish; about 0.4 s before capture, the dolphin clicks become more rapid to form a second phase, the terminal buzz; at or just before capture, the buzz turns to an emotional squeal (the victory squeal), which may last 0.2 to 20 s after capture. The squeals are pulse bursts that vary in duration, peak frequency and amplitude. The victory squeal may be a reflection of emotion triggered by brain dopamine release. It may also affect prey to ease capture and/or it may be a way to communicate the presence of food to other dolphins. Dolphins also use whistles as communication or social sounds. Whistling during sonar clicking suggests that dolphins may be adept at doing two things at once. We know that dolphin brain hemispheres may sleep independently. Our results suggest that the two dolphin brain hemispheres may also act independently in communication. © 2015. Published by The Company of Biologists Ltd.

The Songbird as a Percussionist: Syntactic Rules for Non-Vocal Sound and Song Production in Java Sparrows

PubMed Central

Soma, Masayo; Mori, Chihiro

2015-01-01

Music and dance are two remarkable human characteristics that are closely related. Communication through integrated vocal and motional signals is also common in the courtship displays of birds. The contribution of songbird studies to our understanding of vocal learning has already shed some light on the cognitive underpinnings of musical ability. Moreover, recent pioneering research has begun to show how animals can synchronize their behaviors with external stimuli, like metronome beats. However, few studies have applied such perspectives to unraveling how animals can integrate multimodal communicative signals that have natural functions. Additionally, studies have rarely asked how well these behaviors are learned. With this in mind, here we cast a spotlight on an unusual animal behavior: non-vocal sound production associated with singing in the Java sparrow (Lonchura oryzivora), a songbird. We show that male Java sparrows coordinate their bill-click sounds with the syntax of their song-note sequences, similar to percussionists. Analysis showed that they produced clicks frequently toward the beginning of songs and before/after specific song notes. We also show that bill-clicking patterns are similar between social fathers and their sons, suggesting that these behaviors might be learned from models or linked to learning-based vocalizations. Individuals untutored by conspecifics also exhibited stereotypical bill-clicking patterns in relation to song-note sequence, indicating that while the production of bill clicking itself is intrinsic, its syncopation appears to develop with songs. This paints an intriguing picture in which non-vocal sounds are integrated with vocal courtship signals in a songbird, a model that we expect will contribute to the further understanding of multimodal communication. PMID:25992841

Blind people are more sensitive than sighted people to binaural sound-location cues, particularly inter-aural level differences.

PubMed

Nilsson, Mats E; Schenkman, Bo N

2016-02-01

Blind people use auditory information to locate sound sources and sound-reflecting objects (echolocation). Sound source localization benefits from the hearing system's ability to suppress distracting sound reflections, whereas echolocation would benefit from "unsuppressing" these reflections. To clarify how these potentially conflicting aspects of spatial hearing interact in blind versus sighted listeners, we measured discrimination thresholds for two binaural location cues: inter-aural level differences (ILDs) and inter-aural time differences (ITDs). The ILDs or ITDs were present in single clicks, in the leading component of click pairs, or in the lagging component of click pairs, exploiting processes related to both sound source localization and echolocation. We tested 23 blind (mean age = 54 y), 23 sighted-age-matched (mean age = 54 y), and 42 sighted-young (mean age = 26 y) listeners. The results suggested greater ILD sensitivity for blind than for sighted listeners. The blind group's superiority was particularly evident for ILD-lag-click discrimination, suggesting not only enhanced ILD sensitivity in general but also increased ability to unsuppress lagging clicks. This may be related to the blind person's experience of localizing reflected sounds, for which ILDs may be more efficient than ITDs. On the ITD-discrimination tasks, the blind listeners performed better than the sighted age-matched listeners, but not better than the sighted young listeners. ITD sensitivity declines with age, and the equal performance of the blind listeners compared to a group of substantially younger listeners is consistent with the notion that blind people's experience may offset age-related decline in ITD sensitivity. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

Sound localization in noise in hearing-impaired listeners.

PubMed

Lorenzi, C; Gatehouse, S; Lever, C

1999-06-01

The present study assesses the ability of four listeners with high-frequency, bilateral symmetrical sensorineural hearing loss to localize and detect a broadband click train in the frontal-horizontal plane, in quiet and in the presence of a white noise. The speaker array and stimuli are identical to those described by Lorenzi et al. (in press). The results show that: (1) localization performance is only slightly poorer in hearing-impaired listeners than in normal-hearing listeners when noise is at 0 deg azimuth, (2) localization performance begins to decrease at higher signal-to-noise ratios for hearing-impaired listeners than for normal-hearing listeners when noise is at +/- 90 deg azimuth, and (3) the performance of hearing-impaired listeners is less consistent when noise is at +/- 90 deg azimuth than at 0 deg azimuth. The effects of a high-frequency hearing loss were also studied by measuring the ability of normal-hearing listeners to localize the low-pass filtered version of the clicks. The data reproduce the effects of noise on three out of the four hearing-impaired listeners when noise is at 0 deg azimuth. They reproduce the effects of noise on only two out of the four hearing-impaired listeners when noise is at +/- 90 deg azimuth. The additional effects of a low-frequency hearing loss were investigated by attenuating the low-pass filtered clicks and the noise by 20 dB. The results show that attenuation does not strongly affect localization accuracy for normal-hearing listeners. Measurements of the clicks' detectability indicate that the hearing-impaired listeners who show the poorest localization accuracy also show the poorest ability to detect the clicks. The inaudibility of high frequencies, "distortions," and reduced detectability of the signal are assumed to have caused the poorer-than-normal localization accuracy for hearing-impaired listeners.

A study of the radiosynthesis of fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ and its application in labeling 1,2,3-triazole analogs obtained by click chemistry.

PubMed

Wang, Cheng; Zhou, Wei; Yu, Junfeng; Zhang, Lan; Wang, Ni

2012-01-01

To optimize the conditions for the preparation of the organometallic precursor fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ and to synthesize the radiolabeling compounds of tricarbonyl rhenium. 1,2,3-Triazole analogs were synthesized by click chemistry and labeled with fac-[ReCO₃(H₂O)₃]Br and fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺. The aim was to improve the methods for the synthesis of ¹⁸⁸Re-labeled radiopharmaceuticals for therapy. With potassium boranocarbonate as the CO source and ammonia borane as the reducing agent, fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ was synthesized, and the click chemistry method was used to prepare the tricarbonyl rhenium complex. At the optimal reaction condition (the amounts of K₂[H₃BCO₂] and BH₃·NH₃ are 5 and 5 mg, respectively; reaction temperature is 75°C; and reaction time is 15 min), the radiochemical yields were 90%, and the labeling yield of bis(pyridin-2-ylmethyl) amine with fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ was more than 99% in 1 h at 75°C; the conjugation yields of triazole analog obtained by click chemistry with 'cold' and 'radio' tricarbonyl rhenium were more than 80%. The organometallic precursor fac-[¹⁸⁸ReCO₃(H₂O)₃]⁺ was prepared under optimal reaction conditions with a yield of 90%, and the triazole analogs synthesized by click chemistry were suitable ligands for tricarbonyl rhenium.

Click chemistry reactions in medicinal chemistry: applications of the 1,3-dipolar cycloaddition between azides and alkynes.

PubMed

Tron, Gian Cesare; Pirali, Tracey; Billington, Richard A; Canonico, Pier Luigi; Sorba, Giovanni; Genazzani, Armando A

2008-03-01

In recent years, there has been an ever-increasing need for rapid reactions that meet the three main criteria of an ideal synthesis: efficiency, versatility, and selectivity. Such reactions would allow medicinal chemistry to keep pace with the multitude of information derived from modern biological screening techniques. The present review describes one of these reactions, the 1,3-dipolar cycloaddition ("click-reaction") between azides and alkynes catalyzed by copper (I) salts. The simplicity of this reaction and the ease of purification of the resulting products have opened new opportunities in generating vast arrays of compounds with biological potential. The present review will outline the accomplishments of this strategy achieved so far and outline some of medicinal chemistry applications in which click-chemistry might be relevant in the future. (c) 2007 Wiley Periodicals, Inc.

Copper-free click chemistry for dynamic in vivo imaging

PubMed Central

Baskin, Jeremy M.; Prescher, Jennifer A.; Laughlin, Scott T.; Agard, Nicholas J.; Chang, Pamela V.; Miller, Isaac A.; Lo, Anderson; Codelli, Julian A.; Bertozzi, Carolyn R.

2007-01-01

Dynamic imaging of proteins in live cells is routinely performed by using genetically encoded reporters, an approach that cannot be extended to other classes of biomolecules such as glycans and lipids. Here, we report a Cu-free variant of click chemistry that can label these biomolecules rapidly and selectively in living systems, overcoming the intrinsic toxicity of the canonical Cu-catalyzed reaction. The critical reagent, a substituted cyclooctyne, possesses ring strain and electron-withdrawing fluorine substituents that together promote the [3 + 2] dipolar cycloaddition with azides installed metabolically into biomolecules. This Cu-free click reaction possesses comparable kinetics to the Cu-catalyzed reaction and proceeds within minutes on live cells with no apparent toxicity. With this technique, we studied the dynamics of glycan trafficking and identified a population of sialoglycoconjugates with unexpectedly rapid internalization kinetics. PMID:17942682

Monoglycoconjugated phthalocyanines: effect of sugar and linkage on photodynamic activity.

PubMed

Lafont, Dominique; Zorlu, Yunus; Savoie, Huguette; Albrieux, Florian; Ahsen, Vefa; Boyle, Ross W; Dumoulin, Fabienne

2013-09-01

Click chemistry can be advantageously used to graft carbohydrates on phthalocyanines which are potent photosensitisers, but the effect of the presence of triazole moieties on photodynamic efficiency was not investigated systematically to date. The nature and linkage of the sugar were investigated in order to define structure-activity relationships. Two sets of monoglycoconjugated water-soluble phthalocyanines have been designed and their photodynamic activity and uptake investigated in HT-29 human colon adenocarcinoma cells. Carbohydrates: galactose, mannose or lactose were grafted onto Zn(II) phthalocyanines either by glycosylation or by click reaction. The triazole linkage formed by click conjugation lowered the biological efficiency for mannose and galactose, compared to classical glycosylation grafting. The mannose conjugate formed by glycosylation was the most photodynamically active, without correlation with the photosensitiser cell uptake. Copyright © 2012 Elsevier B.V. All rights reserved.

Predation by killer whales (Orcinus orca) and the evolution of whistle loss and narrow-band high frequency clicks in odontocetes.

PubMed

Morisaka, T; Connor, R C

2007-07-01

A disparate selection of toothed whales (Odontoceti) share striking features of their acoustic repertoires including the absence of whistles and high frequency but weak (low peak-to-peak source level) clicks that have a relatively long duration and a narrow bandwidth. The non-whistling, high frequency click species include members of the family Phocoenidae, members of one genus of delphinids, Cephalorhynchus, the pygmy sperm whale, Kogia breviceps, and apparently the sole member of the family Pontoporiidae. Our review supports the 'acoustic crypsis' hypothesis that killer whale predation risk was the primary selective factor favouring an echolocation and communication system in cephalorhynchids, phocoenids and possibly Pontoporiidae and Kogiidae restricted to sounds that killer whales hear poorly or not at all (< 2 and > 100 kHz).

Use of the PIXEL method to investigate gas adsorption in metal–organic frameworks† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6ce00555a Click here for additional data file. Click here for additional data file. Click here for additional data file.

PubMed Central

Maloney, Andrew G. P.; Wood, Peter A.

2016-01-01

PIXEL has been used to perform calculations of adsorbate-adsorbent interaction energies between a range of metal–organic frameworks (MOFs) and simple guest molecules. Interactions have been calculated for adsorption between MOF-5 and Ar, H2, and N2; Zn2(BDC)2(TED) (BDC = 1,4-benzenedicarboxylic acid, TED = triethylenediamine) and H2; and HKUST-1 and CO2. The locations of the adsorption sites and the calculated energies, which show differences in the Coulombic or dispersion characteristic of the interaction, compare favourably to experimental data and literature energy values calculated using density functional theory. PMID:28496380

Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry

PubMed Central

Witte, Martin D.; Theile, Chris; Wu, Tongfei; Guimaraes, Carla P.; Blom, Annet E. M.; Ploegh, Hidde L.

2014-01-01

Chimeric proteins, including bi-specific antibodies, are biological tools with therapeutic applications. Genetic fusion and ligation methods allow the creation of N-to-C and C-toN fused recombinant proteins, but not the majority of non-template encoded fusions. The present protocol describes a simple procedure for the production of unnaturally linked N-to-N and C-to-C chimeric proteins. Equipping the N-terminus or C-terminus of the proteins of interest with a set of click handles using sortase A, followed by a click reaction, establishes unnatural N-to-N and C-to-C (hetero)dimer linked fusions. If the peptides, sortase A, and the proteins of interest are in hand, the unnaturally fused proteins can be obtained in 3–4 days. PMID:23989675

Double immunohistochemical staining with MUC4/p53 is useful in the distinction of pancreatic adenocarcinoma from chronic pancreatitis: a tissue microarray-based study.

PubMed

Bhardwaj, Atul; Marsh, William L; Nash, Jason W; Barbacioru, Catalin C; Jones, Susie; Frankel, Wendy L

2007-04-01

Immunohistochemical stains have been used for the distinction of pancreatic adenocarcinoma from chronic pancreatitis. To determine if a double stain for MUC/p53 improved specificity and sensitivity for distinction of pancreatic adenocarcinoma from chronic pancreatitis by comparing maspin, mucin 4 (MUC4), p53, Smad4, and the double stain MUC4/p53. Seventy-four pancreatic adenocarcinomas and 19 chronic pancreatitis cases were retrieved from archival files. Tissue cores were arrayed to create a tissue microarray of 2-mm cores. Sections were stained with antibodies against maspin, MUC4, p53, and Smad4. Additionally, a 2-color, double stain for MUC4 and p53 was developed and evaluated. Five percent or greater staining in either of the cores was considered positive. Intensity (0, 1, 2) and extent (%) of tumor cells staining was also determined. The sensitivity for distinction of pancreatic adenocarcinoma from chronic pancreatitis with maspin, MUC4, p53, and Smad4 was 90%, 77%, 60%, and 63%, respectively; the specificity was 67%, 78%, 88%, and 88%, respectively. When MUC4 and p53 were combined in a double stain, and positive staining for either considered a positive result, the sensitivity increased to 96% but specificity was 73%. When immunoreactivity for both antibodies was necessary for a positive result, sensitivity fell to 39% but specificity was 100%. No correlation was found between intensity or extent of staining with any of the individual stains and tumor differentiation. The double immunohistochemical stain for MUC4/p53 can be a useful diagnostic tool in conjunction with the hematoxylin-eosin-stained section for pancreatic adenocarcinoma, particularly when limited tumor is available for multiple stains.

The Effects of Behavioral Change in Response to Acoustic Disturbance on the Health of the Population of Blainville’s Beaked Whales (Mesoplodon densirostris) in the Tongue of the Ocean

DTIC Science & Technology

2014-09-30

acoustic data from detection of Md echolocation clicks and sonar, observational data from expert surface observers for group size, surface behavior...on-axis echolocation click source level is in excess of 200 dB), it is therefore highly likely that a GVP will be detected on more than one

"Click" saccharide/beta-lactam hybrids for lectin inhibition.

PubMed

Palomo, Claudio; Aizpurua, Jesus M; Balentová, Eva; Azcune, Itxaso; Santos, J Ignacio; Jiménez-Barbero, Jesús; Cañada, Javier; Miranda, José Ignacio

2008-06-05

Hybrid glycopeptide beta-lactam mimetics designed to bind lectins or carbohydrate recognition domains in selectins have been prepared according to a "shape-modulating linker" design. This approach was implemented using the azide-alkyne "click" cycloaddition reaction, and as shown by NMR/MD experiments, binding of the resulting mimetics to Ulex Europaeus Lectin-1 (UEL-1) occurred after a "bent-to-extended" conformational change around a partially rotatable triazolylmethylene moiety.

The Metabolic Cost of Click Production in Bottlenose Dolphins

DTIC Science & Technology

2014-09-30

America 64, 411-422. Holt, M.M., Noren, D.P., Veirs, V., Emmons, C.K., Veirs, S. 2009. Speaking up: Killer whales (Orcinus orca) increase their call...2007. Recording and quantification of ultrasonic echolocation clicks from free-ranging toothed whales . Deep-Sea Research I 54, 1421-1444. 15...Individual right whales call louder in increased environmental noise. Biology Letters published online 7 July 2010 doi: 10.1098/rsbl.2010.0451

Low-frequency components in harbor porpoise (Phocoena phocoena) clicks: communication signal, by-products, or artifacts?

PubMed

Hansen, M; Wahlberg, M; Madsen, P T

2008-12-01

Underwater sound signals for biosonar and communication normally have different source properties to serve the purposes of generating efficient acoustic backscatter from small objects or conveying information to conspecifics. Harbor porpoises (Phocoena phocoena) are nonwhistling toothed whales that produce directional, narrowband, high-frequency (HF) echolocation clicks. This study tests the hypothesis that their 130 kHz HF clicks also contain a low-frequency (LF) component more suited for communication. Clicks from three captive porpoises were analyzed to quantify the LF and HF source properties. The LF component is 59 (S.E.M=1.45 dB) dB lower than the HF component recorded on axis, and even at extreme off-axis angles of up to 135 degrees , the HF component is 9 dB higher than the LF component. Consequently, the active space of the HF component will always be larger than that of the LF component. It is concluded that the LF component is a by-product of the sound generator rather than a dedicated pulse produced to serve communication purposes. It is demonstrated that distortion and clipping in analog tape recorders can explain some of the prominent LF components reported in earlier studies, emphasizing the risk of erroneous classification of sound types based on recording artifacts.

Vinyl functionalized silica hybrid monolith-based trypsin microreactor for on line digestion and separation via thiol-ene "click" strategy.

PubMed

Chen, Yingzhuang; Wu, Minghuo; Wang, Keyi; Chen, Bo; Yao, Shouzhuo; Zou, Hanfa; Nie, Lihua

2011-11-04

A novel thiol-ene "click" strategy for the preparation of monolithic trypsin microreactor was proposed. The hybrid organic-inorganic monolithic capillary column with ene-functionality was fabricated by sol-gel process using tetramethoxysilane (TMOS) and γ-methacryloxypropyltrimethoxysilane (γ-MAPS) as precursors. The disulfide bonds of trypsin were reduced to form free thiol groups. Then the trypsin containing free thiol groups was attached on the γ-MAPS hybrid monolithic column with ene-functionality via thiol-ene click chemistry to form a trypsin microreactor. The activity of the trypsin microreactor was characterized by detecting the substrate (Nα-p-tosyl-L-arginine methyl ester hydrochloride, TAME) and the product (Nα-p-tosyl-L-arginine, TA) with on-line capillary zone electrophoresis. After investigating various synthesizing conditions, it was found that the microreactor with poly(N,N'-methylenebisacrylamide) as spacer can deliver the highest activity, yielding a rapid reaction rate. After repeatedly sampling and analyzing for 100 times, the monolithic trypsin microreactor still remained 87.5% of its initial activity. It was demonstrated that thiol-ene "click" strategy for the construction of enzyme microreactor is a promising method for the highly selective immobilization of proteins under mild conditions, especially enzymes with free thiol radicals. Copyright © 2011 Elsevier B.V. All rights reserved.

Multifractal detrended fluctuation analysis to characterize phase couplings in seahorse (Hippocampus kuda) feeding clicks.

PubMed

Haris, K; Chakraborty, Bishwajit; Menezes, A; Sreepada, R A; Fernandes, W A

2014-10-01

Nonlinear phenomena in animal vocalizations fundamentally includes known features, namely, frequency jump, subharmonics, biphonation, and deterministic chaos. In the present study, the multifractal detrended fluctuation analysis (MFDFA) has been employed to characterize the phase couplings revealed in the feeding clicks of Hippocampus kuda yellow seahorse. The fluctuation function Fq(s), generalized Hurst exponent h(q), multifractal scaling exponent τ(q), and the multifractal spectrum f(α) calculated in the procedure followed were analyzed to comprehend the underlying nonlinearities in the seahorse clicks. The analyses carried out reveal long-range power-law correlation properties in the data, substantiating the multifractal behavior. The resulting h(q) spectrum exhibits a distinct characteristic pattern in relation to the seahorse sex and size, and reveals a spectral blind spot in the data that was not possible to detect by conventional spectral analyses. The corresponding multifractal spectrum related width parameter Δh(q) is well clustered, defining the individual seahorse clicks. The highest degree of multifractality is evident in the 18 cm male seahorse, signifying greater heterogeneity. A further comparison between the seahorse body size and weight (wet) with respect to the width parameter Δh(q) and the second-order Hurst exponent h(q=2) underscores the versatility of MFDFA as a robust statistical tool to analyze bioacoustic observations.

P50 suppression in children with selective mutism: a preliminary report.

PubMed

Henkin, Yael; Feinholz, Maya; Arie, Miri; Bar-Haim, Yair

2010-01-01

Evidence suggests that children with selective mutism (SM) display significant aberrations in auditory efferent activity at the brainstem level that may underlie inefficient auditory processing during vocalization, and lead to speech avoidance. The objective of the present study was to explore auditory filtering processes at the cortical level in children with SM. The classic paired-click paradigm was utilized to assess suppression of the P50 event-related potential to the second, of two sequentially-presented clicks, in ten children with SM and 10 control children. A significant suppression of P50 to the second click was evident in the SM group, whereas no suppression effect was observed in controls. Suppression was evident in 90% of the SM group and in 40% of controls, whereas augmentation was found in 10% and 60%, respectively, yielding a significant association between group and suppression of P50. P50 to the first click was comparable in children with SM and controls. The adult-like, mature P50 suppression effect exhibited by children with SM may reflect a cortical mechanism of compensatory inhibition of irrelevant repetitive information that was not properly suppressed at lower levels of their auditory system. The current data extends our previous findings suggesting that differential auditory processing may be involved in speech selectivity in SM.

Variation in the production rate of biosonar signals in freshwater porpoises.

PubMed

Kimura, Satoko; Akamatsu, Tomonari; Wang, Ding; Li, Songhai; Wang, Kexiong; Yoda, Ken

2013-05-01

The biosonar (click train) production rate of ten Yangtze finless porpoises and their behavior were examined using animal-borne data loggers. The sound production rate varied from 0 to 290 click trains per 10-min time interval. Large individual differences were observed, regardless of body size. Taken together, however, sound production did not differ significantly between daytime and nighttime. Over the 172.5 h of analyzed recordings, an average of 99.0% of the click trains were produced within intervals of less than 60 s, indicating that during a 1-min interval, the number of click trains produced by each porpoise was typically greater than one. Most of the porpoises exhibited differences in average swimming speed and depth between day and night. Swimming speed reductions and usage of short-range sonar, which relates to prey-capture attempts, were observed more often during nighttime. However, biosonar appears to be affected not only by porpoise foraging, but also by their sensory environment, i.e., the turbid Yangtze River system. These features will be useful for passive acoustic detection of the porpoises. Calculations of porpoise density or abundance should be conducted carefully because large individual differences in the sound production rate will lead to large estimation error.

Hearing impairment in children with congenital cytomegalovirus (CMV) infection based on distortion product otoacoustic emissions (DPOAE) and brain evoked response audiometry stimulus click (BERA Click) examinations

NASA Astrophysics Data System (ADS)

Airlangga, T. J.; Mangunatmadja, I.; Prihartono, J.; Zizlavsky, S.

2017-08-01

Congenital cytomegalovirus (congenital CMV) infection is a leading factor of nongenetic sensorineural hearing loss in children. Hearing loss caused by CMV infection does not have a pathognomonic configuration hence further research is needed. The development of knowledge on hearing loss caused by congenital CMV infection is progressing in many countries. Due to a lack of research in the context of Indonesia, this study assesses hearing impairment in children with congenital CMV infection in Indonesia, more specifically in the Cipto Mangunkusumo Hospital. Our objective was to profile hearing impairment in children 0-5 years of age with congenital CMV infection using Distortion Product Otoacoustic Emissions (DPOAE) and Brain Evoked Response Audiometry Stimulus Click (BERA Click) examinations. This cross-sectional study was conducted in the Cipto Mangunkusum Hospital from November, 2015 to May 2016 with 27 children 0-5 years of age with congenital CMV infection. Of individual ears studied, 58.0% exhibited sensorineural hearing loss. There was a significant relationship between developmental delay and incidence of sensorineural hearing loss. Subjects with a developmental delay were 6.57 times more likely (CI 95%; 1.88-22.87) to experience sensorineural hearing loss. Congenital CMV infection has an important role in causing sensorineural hearing loss in children.

Spatio-temporal variation in click production rates of beaked whales: Implications for passive acoustic density estimation.

PubMed

Warren, Victoria E; Marques, Tiago A; Harris, Danielle; Thomas, Len; Tyack, Peter L; Aguilar de Soto, Natacha; Hickmott, Leigh S; Johnson, Mark P

2017-03-01

Passive acoustic monitoring has become an increasingly prevalent tool for estimating density of marine mammals, such as beaked whales, which vocalize often but are difficult to survey visually. Counts of acoustic cues (e.g., vocalizations), when corrected for detection probability, can be translated into animal density estimates by applying an individual cue production rate multiplier. It is essential to understand variation in these rates to avoid biased estimates. The most direct way to measure cue production rate is with animal-mounted acoustic recorders. This study utilized data from sound recording tags deployed on Blainville's (Mesoplodon densirostris, 19 deployments) and Cuvier's (Ziphius cavirostris, 16 deployments) beaked whales, in two locations per species, to explore spatial and temporal variation in click production rates. No spatial or temporal variation was detected within the average click production rate of Blainville's beaked whales when calculated over dive cycles (including silent periods between dives); however, spatial variation was detected when averaged only over vocal periods. Cuvier's beaked whales exhibited significant spatial and temporal variation in click production rates within vocal periods and when silent periods were included. This evidence of variation emphasizes the need to utilize appropriate cue production rates when estimating density from passive acoustic data.

Short-term Efficacy of Click City®: Tobacco: Changing Etiological Mechanisms Related to the Onset of Tobacco Use

PubMed Central

Andrews, Judy A.; Gordon, Judith S.; Hampson, Sarah H.; Christiansen, Steven M.; Gunn, Barbara; Slovic, Paul; Severson, Herbert H.

2011-01-01

This paper described the short-term results from an ongoing randomized controlled efficacy study of Click City®: Tobacco, a tobacco prevention program designed for 5th graders, with a booster in sixth grade. Click City®: Tobacco is an innovative school-based prevention program delivered via an intranet, a series of linked computers with a single server. The components of the program target theoretically based and empirically supported etiological mechanisms predictive of future willingness and intentions to use tobacco and initiation of tobacco use. Each component was designed to change one or more etiological mechanisms and was empirically evaluated in the laboratory prior to inclusion in the program. Short-term results from 47 elementary schools (24 schools who used Click City®: Tobacco, and 23 who continued with their usual curriculum) showed change in intentions and willingness to use tobacco from baseline to one-week following the completion of the 5th grade sessions. The results demonstrate the short-term efficacy of this program and suggest that experimentally evaluating components prior to including them in the program contributed to the efficacy of the program. The program was most efficacious for students who were most at risk. PMID:21286810

Enzyme-free and isothermal detection of microRNA based on click-chemical ligation-assisted hybridization coupled with hybridization chain reaction signal amplification.

PubMed

Oishi, Motoi

2015-05-01

An enzyme-free and isothermal microRNA (miRNA) detection method has been developed based on click-chemical ligation-assisted hybridization coupled with hybridization chain reaction (HCR) on magnetic beads (MBs). The click-chemical ligation between an azide-modified probe DNA and a dibenzocyclooctyne-modified probe DNA occurred through the hybridization of target miRNA (miR-141). HCR on MBs was performed by the addition of DNA hairpin monomers (H1 and H2). After magnetic separation and denaturation/rehybridization of HCR products ([H1/H2] n ), the resulting HCR products were analyzed by the fluorescence emitted from an intercalative dye, allowing amplification of the fluorescent signal. The proposed assay had a limit of detection of 0.55 fmol, which was 230-fold more sensitive than that of the HCR on the MBs coupled with a conventional sandwich hybridization assay (without click-chemical ligation) (limit of detection 127 fmol). Additionally, the proposed assay could discriminate between miR-141 and other miR-200 family members. In contrast to quantitative reverse transcription polymerase chain reaction techniques using enzymes and thermal cycling, this is an enzyme-free assay that can be conducted under isothermal conditions and can specifically detect miR-141 in fetal bovine serum.

Characterization of luciferases and its paralogue in the Panamanian luminous click beetle Pyrophorus angustus: a click beetle luciferase lacks the fatty acyl-CoA synthetic activity.

PubMed

Oba, Yuichi; Kumazaki, Mizuho; Inouye, Satoshi

2010-02-15

Two luciferase genes (dPaLuc and vPaLuc) and one paralogue of luciferase (PaLL) were isolated from the Panamanian luminous click beetle, Pyrophorus angustus (Elateridae, Pyrophorinae). The transcripts of dPaLuc and vPaLuc were predominantly detected in the body parts with dorsal photophore and ventral photophore, respectively, and the transcript of PaLL was detected in both parts. The gene products of dPaLuc and vPaLuc possessed luminescence activity with firefly luciferin (lambda(max)=536 and 566 nm, respectively) but did not show significant activity of fatty acyl-CoA synthesis. On the other hand, the gene product of PaLL had fatty acyl-CoA synthetic activity with very weak luminescence activity. The catalytic properties of click beetle luciferase are different from our previous results that firefly luciferase has both luminescence activity and fatty acyl-CoA synthetic activity. These results suggested that the ancestral fatty acyl-CoA synthetase in the Pyrophorinae lineage has undergone gene duplication event, followed by specialization of one copy in luciferase. Subsequently, the luciferase was duplicated again and the two copies diverged in their luminescent color and expression pattern. Copyright 2009 Elsevier B.V. All rights reserved.

Reverberation enhances onset dominance in sound localization.

PubMed

Stecker, G Christopher; Moore, Travis M

2018-02-01

Temporal variation in sensitivity to sound-localization cues was measured in anechoic conditions and in simulated reverberation using the temporal weighting function (TWF) paradigm [Stecker and Hafter (2002). J. Acoust. Soc. Am. 112, 1046-1057]. Listeners judged the locations of Gabor click trains (4 kHz center frequency, 5-ms interclick interval) presented from an array of loudspeakers spanning 360° azimuth. Targets ranged ±56.25° across trials. Individual clicks within each train varied by an additional ±11.25° to allow TWF calculation by multiple regression. In separate conditions, sounds were presented directly or in the presence of simulated reverberation: 13 orders of lateral reflection were computed for a 10 m × 10 m room ( RT 60 ≊300 ms) and mapped to the appropriate locations in the loudspeaker array. Results reveal a marked increase in perceptual weight applied to the initial click in reverberation, along with a reduction in the impact of late-arriving sound. In a second experiment, target stimuli were preceded by trains of "conditioner" sounds with or without reverberation. Effects were modest and limited to the first few clicks in a train, suggesting that impacts of reverberant pre-exposure on localization may be limited to the processing of information from early reflections.

[The influence of click polarity and stimulation rate on the auditory evoked response in relation to age].

PubMed

Tarantino, V; Stura, M; Raspino, M; Conrad, E; Porcu, A

1989-01-01

In order to study the changes which occur in phase of the click stimulus and its relation to the stimulus repetition rate on the auditory brainstem response (ABR) as a function of age, the Authors recorded the ABR from the scalp's surface of 10 newborns and 40 infants, 3 months, 6 months, 1 year and 3 years old as well as from 10 normal adults. The stimulus was a square wave of 0.1 msec duration and 90 dBHL level. The stimulus equipment was calibrated twice under visual inspection to ensure that the C and R clicks resulted in an initial membrane deflection toward and from the ear drum respectively. No significant differences could be found for the latencies and amplitude in the C-R comparison. However, the mean values of the complete group of test subjects showed most intraindividual stability for the conventional click stimulation. The latency of the ABR with excitation of the cochlea seemed to be mainly determined by the internal oscillation sequence in the cochlea and not by the stimulus polarity. The amplitudes and latencies of the ABR components tend to decrease when the stimulus rate increases and the age decreases. The importance of the stimulus characteristics is discussed and some suggestions for clinical use of ABR are made.

VizieR Online Data Catalog: Orbits based on speckle interferometry at SOAR (Tokovinin, 2016)

NASA Astrophysics Data System (ADS)

Tokovinin, A.

2017-03-01

This paper presents new or updated orbits for 55 binary systems or subsystems. It is based on speckle interferometric measurements made at the 4.1m Southern Astrophyisical Research (SOAR) telescope combined with archival data collected in the Washington Double Star Catalog (WDS; Mason et al. 2001-2014, Cat. B/wds). Table1 lists the orbital elements and their errors in common notation. Individual observations and residuals are listed in Table2. It contains still unpublished measures made at SOAR in 2016, while some published SOAR measures were reprocessed. Table3 provides additional information. (4 data files).

On the absence of radio haloes in clusters with double relics

NASA Astrophysics Data System (ADS)

Bonafede, A.; Cassano, R.; Brüggen, M.; Ogrean, G. A.; Riseley, C. J.; Cuciti, V.; de Gasperin, F.; Golovich, N.; Kale, R.; Venturi, T.; van Weeren, R. J.; Wik, D. R.; Wittman, D.

2017-09-01

Pairs of radio relics are believed to form during cluster mergers, and are best observed when the merger occurs in the plane of the sky. Mergers can also produce radio haloes, through complex processes likely linked to turbulent re-acceleration of cosmic ray electrons. However, only some clusters with double relics also show a radio halo. Here, we present a novel method to derive upper limits on the radio halo emission, and analyse archival X-ray Chandra data, as well as galaxy velocity dispersions and lensing data, in order to understand the key parameter that switches on radio halo emission. We place upper limits on the halo power below the P1.4 GHz-M500 correlation for some clusters, confirming that clusters with double relics have different radio properties. Computing X-ray morphological indicators, we find that clusters with double relics are associated with the most disturbed clusters. We also investigate the role of different mass-ratios and time-since-merger. Data do not indicate that the merger mass-ratio has an impact on the presence or absence of radio haloes (the null hypothesis that the clusters belong to the same group cannot be rejected). However, the data suggest that the absence of radio haloes could be associated with early and late mergers, but the sample is too small to perform a statistical test. Our study is limited by the small number of clusters with double relics. Future surveys with LOFAR, ASKAP, MeerKat and SKA will provide larger samples to better address this issue.

Baseline coastal oblique aerial photographs collected from Key Largo, Florida, to the Florida/Georgia border, September 5-6, 2014

USGS Publications Warehouse

Morgan, Karen L. M.

2015-09-14

In addition to the photographs, a Google Earth Keyhole Markup Language (KML) file is provided and can be used to view the images by clicking on the marker and then clicking on either the thumbnail or the link above the thumbnail. The KML files were created using the photographic navigation files. These KML files can be found in the kml folder.

High Resolution Frequency Swept Imaging.

DTIC Science & Technology

1983-09-30

Appendix XI). Preliminary ex- perimental results obtained using ultrasound are extremely encouraging. These -12- ’!" , ,a, -’ ’- : "--’ .,"U...of broad-band nature. They are either In the form of relatively long chirps ( whistles ), impulse like pings or clicks of less than lmsec duration and...Bottlenose Porpoise: A Study of Whistles and Clicks", Zoologica, Vol. 47, 1962, pp. 121-128. L 2 I ._ 4! i APPENDIX II " HBOLOCRAPHY, WAVE-ILE GTH DIVERSITY

ASW Reach-Back Cell Oceanography Analysis System (ARCOAS) Version 3 User’s Guide

DTIC Science & Technology

2012-02-24

65 Table of Figures Figure 2.1: Warning dialog box indicating ActiveX ...Click OK in response to the message box indicating that ActiveX controls are being used by the application (Figure 2.1). Figure 2.1: Warning...dialog box indicating ActiveX controls could be unsafe. 3. Open an existing map or create a new empty map. 4. Start ARCOAS by clicking the ARCOAS

Structure-Based Design of Inhibitors to the Cytotoxin Ricin

DTIC Science & Technology

2006-07-01

markers for the receptor may be predicted by these programs. To follow up on this experiment, we began to screen a large commercial data base, the...of the Huisgen thermal [3+2] cycloaddition reaction (Table 1). The advantages of this click chemistry approach include the potential for favorable...41, 2113-2116. Hartmuth C. Kolb, M. G. F. K. B. S. (2001). Click Chemistry: Diverse Chemical Function from a Few Good Reactions . Angewandte