Final Environmental Assessment: Base-Wide Building Demolition Arnold Air Force Base, Tennessee

DTIC Science & Technology

2006-02-01

Building • Engine Test Facility ( ETF )-B Exhauster • ETF -A Airside • ETF -A Exhauster • ETF -A Reefer • CE Facility • Rocket Storage • Von Karman Gas...Executive Order ESA Endangered Species Act ETF Engine Test Facility FamCamp Family Camping Area P:\\ARNOLDAFB\\333402DO42COMPLIANCE\\DEMOLITION...Fabrication Shop • Natural Resources Building • Salt Storage Building • Administration Building • Engine Test Facility ( ETF )-B Exhauster • ETF -A

Radionuclide Retention Mechanisms in Secondary Waste-Form Testing: Phase II

DOE Office of Scientific and Technical Information (OSTI.GOV)

Um, Wooyong; Valenta, Michelle M.; Chung, Chul-Woo

2011-09-26

This report describes the results from laboratory tests performed at Pacific Northwest National Laboratory (PNNL) for Washington River Protection Solutions (WRPS) to evaluate candidate stabilization technologies that have the potential to successfully treat liquid secondary waste stream effluents produced by the Hanford Tank Waste Treatment and Immobilization Plant (WTP). WRPS is considering the design and construction of a Solidification Treatment Unit (STU) for the Effluent Treatment Facility (ETF) at Hanford. The ETF, a multi-waste, treatment-and-storage unit that has been permitted under the Resource Conservation and Recovery Act (RCRA), can accept dangerous, low-level, and mixed wastewaters for treatment. The STU needsmore » to be operational by 2018 to receive secondary liquid waste generated during operation of the WTP. The STU will provide the additional capacity needed for ETF to process the increased volume of secondary waste expected to be produced by WTP. This report on radionuclide retention mechanisms describes the testing and characterization results that improve understanding of radionuclide retention mechanisms, especially for pertechnetate, {sup 99}TcO{sub 4}{sup -} in four different waste forms: Cast Stone, DuraLith alkali aluminosilicate geopolymer, encapsulated fluidized bed steam reforming (FBSR) product, and Ceramicrete phosphate bonded ceramic. These data and results will be used to fill existing data gaps on the candidate technologies to support a decision-making process that will identify a subset of the candidate waste forms that are most promising and should undergo further performance testing.« less

Report on Analyses of WAC Samples of Evaporator Overheads - 2004

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oji, L

2005-03-18

In November and December of 2004, the Tank Farm submitted annual samples from 2F, 2H and 3H Evaporator Overhead streams for characterization to verify compliance with the new Effluent Treatment Facility (ETF) Waste Acceptance Criteria (WAC) and to look for organic species. With the exception of slightly high ammonia in the 2F evaporator overheads and high radiation control guide number for the 3H and 2F evaporator overhead samples, all the overheads samples were found to be in compliance with the Effluent Treatment Facility WAC. The ammonium concentration in the 2F-evaporator overhead, at 33 mg/L, was above the ETF waste watermore » collection tank (WWCT) limits of 28 mg/L. The RCG Number for the 3H and 2F evaporator samples at, respectively, 1.38E-02 and 8.24E-03 were higher than the WWCT limit of 7.69E-03. The analytical detection limits for americium-241 and radium-226 in the evaporator samples were not consistently met because of low WWCT detection limits and insufficient evaporator samples.« less

Secondary Waste Cementitious Waste Form Data Package for the Integrated Disposal Facility Performance Assessment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cantrell, Kirk J.; Westsik, Joseph H.; Serne, R Jeffrey

A review of the most up-to-date and relevant data currently available was conducted to develop a set of recommended values for use in the Integrated Disposal Facility (IDF) performance assessment (PA) to model contaminant release from a cementitious waste form for aqueous wastes treated at the Hanford Effluent Treatment Facility (ETF). This data package relies primarily upon recent data collected on Cast Stone formulations fabricated with simulants of low-activity waste (LAW) and liquid secondary wastes expected to be produced at Hanford. These data were supplemented, when necessary, with data developed for saltstone (a similar grout waste form used at themore » Savannah River Site). Work is currently underway to collect data on cementitious waste forms that are similar to Cast Stone and saltstone but are tailored to the characteristics of ETF-treated liquid secondary wastes. Recommended values for key parameters to conduct PA modeling of contaminant release from ETF-treated liquid waste are provided.« less

LITERATURE REVIEW ON IMPACT OF GLYCOLATE ON THE 2H EVAPORATOR AND THE EFFLUENT TREATMENT FACILITY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adu-Wusu, K.

2012-05-10

Glycolic acid (GA) is being studied as an alternate reductant in the Defense Waste Processing Facility (DWPF) feed preparation process. It will either be a total or partial replacement for the formic acid that is currently used. A literature review has been conducted on the impact of glycolate on two post-DWPF downstream systems - the 2H Evaporator system and the Effluent Treatment Facility (ETF). The DWPF recycle stream serves as a portion of the feed to the 2H Evaporator. Glycolate enters the evaporator system from the glycolate in the recycle stream. The overhead (i.e., condensed phase) from the 2H Evaporatormore » serves as a portion of the feed to the ETF. The literature search revealed that virtually no impact is anticipated for the 2H Evaporator. Glycolate may help reduce scale formation in the evaporator due to its high complexing ability. The drawback of the solubilizing ability is the potential impact on the criticality analysis of the 2H Evaporator system. It is recommended that at least a theoretical evaluation to confirm the finding that no self-propagating violent reactions with nitrate/nitrites will occur should be performed. Similarly, identification of sources of ignition relevant to glycolate and/or update of the composite flammability analysis to reflect the effects from the glycolate additions for the 2H Evaporator system are in order. An evaluation of the 2H Evaporator criticality analysis is also needed. A determination of the amount or fraction of the glycolate in the evaporator overhead is critical to more accurately assess its impact on the ETF. Hence, use of predictive models like OLI Environmental Simulation Package Software (OLI/ESP) and/or testing are recommended for the determination of the glycolate concentration in the overhead. The impact on the ETF depends on the concentration of glycolate in the ETF feed. The impact is classified as minor for feed glycolate concentrations {le} 33 mg/L or 0.44 mM. The ETF unit operations that will have minor/major impacts are chlorination, pH adjustment, 1st mercury removal, organics removal, 2nd mercury removal, and ion exchange. For minor impacts, the general approach is to use historical process operations data/modeling software like OLI/ESP and/or monitoring/compiled process operations data to resolve any uncertainties with testing as a last resort. For major impacts (i.e., glycolate concentrations > 33 mg/L or 0.44 mM), testing is recommended. No impact is envisaged for the following ETF unit operations regardless of the glycolate concentration - filtration, reverse osmosis, ion exchange resin regeneration, and evaporation.« less

High-level waste tank farm set point document

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anthony, J.A. III

1995-01-15

Setpoints for nuclear safety-related instrumentation are required for actions determined by the design authorization basis. Minimum requirements need to be established for assuring that setpoints are established and held within specified limits. This document establishes the controlling methodology for changing setpoints of all classifications. The instrumentation under consideration involve the transfer, storage, and volume reduction of radioactive liquid waste in the F- and H-Area High-Level Radioactive Waste Tank Farms. The setpoint document will encompass the PROCESS AREA listed in the Safety Analysis Report (SAR) (DPSTSA-200-10 Sup 18) which includes the diversion box HDB-8 facility. In addition to the PROCESS AREASmore » listed in the SAR, Building 299-H and the Effluent Transfer Facility (ETF) are also included in the scope.« less

Liquid secondary waste: Waste form formulation and qualification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cozzi, A. D.; Dixon, K. L.; Hill, K. A.

The Hanford Site Effluent Treatment Facility (ETF) currently treats aqueous waste streams generated during site cleanup activities. When the Hanford Tank Waste Treatment and Immobilization Plant (WTP) begins operations, including Direct Feed Low Activity Waste (DFLAW) vitrification, a liquid secondary waste (LSW) stream from the WTP will need to be treated. The volume of effluent for treatment at the ETF will increase significantly. The powdered salt waste form produced by the ETF will be replaced by a stabilized solidified waste form for disposal in Hanford’s Integrated Disposal Facility (IDF). Washington River Protection Solutions is implementing a Secondary Liquid Waste Immobilizationmore » Technology Development Plan to address the technology needs for a waste form and solidification process to treat the increased volume of waste planned for disposal at the IDF. Waste form testing to support this plan is composed of work in the near term to provide data as input to a performance assessment (PA) for Hanford’s IDF. In 2015, three Hanford Liquid Secondary Waste simulants were developed based on existing and projected waste streams. Using these waste simulants, fourteen mixes of Hanford Liquid Secondary Waste were prepared and tested varying the waste simulant, the water-to-dry materials ratio, and the dry materials blend composition.1 In FY16, testing was performed using a simulant of the EMF process condensate blended with the caustic scrubber—from the Low Activity Waste (LAW) melter—, processed through the ETF. The initial EMF-16 simulant will be based on modeling efforts performed to determine the mass balance of the ETF for the DFLAW.2 The compressive strength of all of the mixes exceeded the target of 3.4 MPa (500 psi) to meet the requirements identified as potential IDF Waste Acceptance Criteria in Table 1 of the Secondary Liquid Waste Immobilization Technology Development Plan.3 The hydraulic properties of the waste forms tested (hydraulic conductivity and water characteristic curves) were comparable to the properties measured on the Savannah River Site (SRS) Saltstone waste form. Future testing should include efforts to first; 1) determine the rate and amount of ammonia released during each unit operation of the treatment process to determine if additional ammonia management is required, then; 2) reduce the ammonia content of the ETF concentrated brine prior to solidification, making the waste more amenable to grouting, or 3) manage the release of ammonia during production and ongoing release during storage of the waste form, or 4) develop a lower pH process/waste form thereby precluding ammonia release.« less

SAR Processing Based On Two-Dimensional Transfer Function

NASA Technical Reports Server (NTRS)

Chang, Chi-Yung; Jin, Michael Y.; Curlander, John C.

1994-01-01

Exact transfer function, ETF, is two-dimensional transfer function that constitutes basis of improved frequency-domain-convolution algorithm for processing synthetic-aperture-radar, SAR data. ETF incorporates terms that account for Doppler effect of motion of radar relative to scanned ground area and for antenna squint angle. Algorithm based on ETF outperforms others.

Secondary Waste Simulant Development for Cast Stone Formulation Testing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Russell, Renee L.; Westsik, Joseph H.; Rinehart, Donald E.

Washington River Protection Solutions, LLC (WRPS) funded Pacific Northwest National Laboratory (PNNL) to conduct a waste form testing program to implement aspects of the Secondary Liquid Waste Treatment Cast Stone Technology Development Plan (Ashley 2012) and the Hanford Site Secondary Waste Roadmap (PNNL 2009) related to the development and qualification of Cast Stone as a potential waste form for the solidification of aqueous wastes from the Hanford Site after the aqueous wastes are treated at the Effluent Treatment Facility (ETF). The current baseline is that the resultant Cast Stone (or grout) solid waste forms would be disposed at the Integratedmore » Disposal Facility (IDF). Data and results of this testing program will be used in the upcoming performance assessment of the IDF and in the design and operation of a solidification treatment unit planned to be added to the ETF. The purpose of the work described in this report is to 1) develop simulants for the waste streams that are currently being fed and future WTP secondary waste streams also to be fed into the ETF and 2) prepare simulants to use for preparation of grout or Cast Stone solid waste forms for testing.« less

ETF Mission Statement document. ETF Design Center team

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1980-04-01

The Mission Statement document describes the results, activities, and processes used in preparing the Mission Statement, facility characteristics, and operating goals for the Engineering Test Facility (ETF). Approximately 100 engineers and scientists from throughout the US fusion program spent three days at the Knoxville Mission Workshop defining the requirements that should be met by the ETF during its operating life. Seven groups were selected to consider one major category each of design and operation concerns. Each group prepared the findings of the assigned area as described in the major sections of this document. The results of the operations discussed mustmore » provide the data, knowledge, experience, and confidence to continue to the next steps beyond the ETF in making fusion power a viable energy option. The results from the ETF mission (operations are assumed to start early in the 1990's) are to bridge the gap between the base of magnetic fusion knowledge at the start of operations and that required to design the EPR/DEMO devices.« less

Liver tumor promoting effect of etofenprox in rats and its possible mechanism of action.

PubMed

Hojo, Yuri; Shiraki, Ayako; Tsuchiya, Takuma; Shimamoto, Keisuke; Ishii, Yuji; Suzuki, Kazuhiko; Shibutani, Makoto; Mitsumori, Kunitoshi

2012-01-01

To investigate the liver tumor-promoting effects of etofenprox (ETF), a pyrethroid-like insecticide, 6 week-old male F344 rats were given an intraperitoneal injection of N-diethylnitrosamine (DEN). After 2 weeks from the DEN treatment, 12 rats per group received a powdered diet containing 0, 0.25, 0.50, or 1.0% ETF for 8 weeks. At the time of 2nd week of ETF administration, all animals were subjected to two-thirds partial hepatectomy (PH). One rat per group except for the 0.25% ETF group died due to surgical operation of PH. The number and area of glutathione S-transferase placental form (GST-P) positive foci significantly increased in the livers of DEN-initiated rats given 0.50% and 1.0% ETF compared with the DEN-alone group. Quantitative real-time RT-PCR analysis revealed that the mRNA expression of phase I enzymes Cyp2b1/2, phase II enzymes such as Akr7a3, Gsta5, Ugt1a6, Nqo1 significantly increased in the DEN+ETF groups. The immunohistochemistry showed the translocation of CAR from the cytoplasm to the nuclei of hepatocytes in the ETF-treated groups. Reactive oxygen species (ROS) production increased in microsomes isolated from the livers of ETF-treated rats, and thiobarbituric acid-reactive substances (TBARS) levels and 8- hydroxy-2-deoxyguanosine (8-OHdG) content significantly increased in all of the ETF-treated groups and DEN+1.0% ETF group, respectively. The results of the present study indicate that ETF has a liver tumor-promoting activity in rats, and suggest that ETF activates the constitutive active/androstane receptor (CAR) and enhances microsomal ROS production, resulting in the upregulation of Nrf2 gene batteries; such an oxidative stress subsequently induces liver tumor-promoting effects by increased cellular proliferation.

Radiation effects on ETFE polymer exposed to glow discharge

NASA Astrophysics Data System (ADS)

Minamisawa, Renato Amaral; Abidzina, Volha; de Almeida, Adelaide; Budak, Satilmis; Tereshko, I.; Elkin, I.; Ila, Daryush

2007-08-01

The polymer ethylenetetrafluoroethylene (ETFE) is composed of alternating ethylene and tetrafluoroethylene segments. Because it has applications in areas such as medical physics and industrial coatings, there is a great interest in surface modification studies of ETFE polymer. When this material is exposed to ionizing radiation it suffers damage that depends on the type, energy and intensity of the irradiation. In order to determine the radiation damage mechanism from exposure to low voltage plasma, ETFE films were exposed to residual gas plasma in glow discharge regime to a fluence of 2 × 1017 ions/cm2. Irradiated films were analyzed with optical absorption photospectrometry, Fourier transform infrared (FTIR) and Raman spectroscopy to determine the chemical nature of the structural changes caused by low energy glow discharge.

Highlights: The ETF in 2002.

ERIC Educational Resources Information Center

European Training Foundation, Turin (Italy).

As part of its mandate to help the European Commission (EC) define, provide, monitor, and evaluate support to vocational education and training (VET) in regions outside the European Union (EU), the European Training Foundation (ETF) provided assistance to the following areas in 2002: Central and Eastern Europe; the New Independent States and…

CORROSION STUDY FOR THE EFFLUENT TREATMENT FACILITY (ETF) CHROME (VI) REDUCTANT SOLUTION USING 304 & 316L STAINLESS STEEL

DOE Office of Scientific and Technical Information (OSTI.GOV)

DUNCAN, J.B.

2007-06-27

The Effluent Treatment Facility has developed a method to regenerate spent resin from the groundwater pump and treat intercepting chrome(VI) plumes (RPP-RPT-32207, Laboratory Study on Regeneration of Spent DOWEX 21K 16-20 Mesh Ion Exchange Resin). Subsequent laboratory studies have shown that the chrome(VI) may be reduced to chrome(III) by titrating with sodium metabisulfite to an oxidation reduction potential (ORP) of +280 mV at a pH of 2. This test plan describes the use of cyclic potentiodynamic polarization and linear polarization techniques to ascertain the electrochemical corrosion and pitting propensity of the 304 and 316L stainless steel in the acidified reducingmore » the solution that will be contained in either the secondary waste receiver tank or concentrate tank.« less

The Fabrication of Nanoimprinted P3HT Nanograting by Patterned ETFE Mold at Room Temperature and Its Application for Solar Cell

NASA Astrophysics Data System (ADS)

Ding, Guangzhu; Wang, Kaixuan; Li, Xiaohui; Chen, Qing; Hu, Zhijun; Liu, Jieping

2016-05-01

Nanoimprinting lithography (NIL) is investigated as a promising method to define nanostructure; however, finding a practical method to achieve large area patterning of conjugated polymer remains a challenge. We demonstrate here that a simple and cost-effective technique is proposed to fabricate the nanoimprinted P3HT nanograting by solvent-assisted room temperature NIL (SART-NIL) method with patterned ETFE film as mold. The patterned ETFE template is produced by embossing ETFE film into a patterned silicon master and is used as template to transfer nanogratings during the SART-NIL process. It indicates that highly reproducible and well-controlled P3HT nanograting film is obtained successfully with feature size of nanogratings ranging from 130 to 700 nm, due to the flexibility, stiffness, and low surface energy of ETFE mold. Moreover, the SART-NIL method using ETFE mold is able to fabricate nanogratings but not to induce the change of molecular orientation within conjugated polymer. The conducting ability of P3HT nanograting in the vertical direction is also not damaged after patterning. Finally, we further apply P3HT nanograting for the fabrication of active layer of OBHJ solar cell device, to investigate the morphology role presented by ETFE mold in device performance. The device performance of OBHJ solar cell is preferential to that of PBHJ device obviously.

The Fabrication of Nanoimprinted P3HT Nanograting by Patterned ETFE Mold at Room Temperature and Its Application for Solar Cell.

PubMed

Ding, Guangzhu; Wang, Kaixuan; Li, Xiaohui; Chen, Qing; Hu, Zhijun; Liu, Jieping

2016-12-01

Nanoimprinting lithography (NIL) is investigated as a promising method to define nanostructure; however, finding a practical method to achieve large area patterning of conjugated polymer remains a challenge. We demonstrate here that a simple and cost-effective technique is proposed to fabricate the nanoimprinted P3HT nanograting by solvent-assisted room temperature NIL (SART-NIL) method with patterned ETFE film as mold. The patterned ETFE template is produced by embossing ETFE film into a patterned silicon master and is used as template to transfer nanogratings during the SART-NIL process. It indicates that highly reproducible and well-controlled P3HT nanograting film is obtained successfully with feature size of nanogratings ranging from 130 to 700 nm, due to the flexibility, stiffness, and low surface energy of ETFE mold. Moreover, the SART-NIL method using ETFE mold is able to fabricate nanogratings but not to induce the change of molecular orientation within conjugated polymer. The conducting ability of P3HT nanograting in the vertical direction is also not damaged after patterning. Finally, we further apply P3HT nanograting for the fabrication of active layer of OBHJ solar cell device, to investigate the morphology role presented by ETFE mold in device performance. The device performance of OBHJ solar cell is preferential to that of PBHJ device obviously.

Review of Potential Candidate Stabilization Technologies for Liquid and Solid Secondary Waste Streams

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pierce, Eric M.; Mattigod, Shas V.; Westsik, Joseph H.

2010-01-30

Pacific Northwest National Laboratory has initiated a waste form testing program to support the long-term durability evaluation of a waste form for secondary wastes generated from the treatment and immobilization of Hanford radioactive tank wastes. The purpose of the work discussed in this report is to identify candidate stabilization technologies and getters that have the potential to successfully treat the secondary waste stream liquid effluent, mainly from off-gas scrubbers and spent solids, produced by the Hanford Tank Waste Treatment and Immobilization Plant (WTP). Down-selection to the most promising stabilization processes/waste forms is needed to support the design of a solidificationmore » treatment unit (STU) to be added to the Effluent Treatment Facility (ETF). To support key decision processes, an initial screening of the secondary liquid waste forms must be completed by February 2010.« less

76 FR 76464 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing of Proposed Rule Change Relating...

Federal Register 2010, 2011, 2012, 2013, 2014

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... Global Allocation ETF; SPDR SSgA Global Allocation ETF; and SPDR SSgA Aggressive Global Allocation ETF... Global Allocation ETF; SPDR SSgA Global Allocation ETF; and SPDR SSgA Aggressive Global Allocation ETF... Allocation ETF; SPDR SSgA Global Allocation ETF; and SPDR SSgA Aggressive Global Allocation ETF (each, a...

The Light Ion Pulsed Power Induction Accelerator for ETF

DTIC Science & Technology

1995-07-01

the technical development necessary to demonstrate scientific and engineering feasibility for fusion energy production with a reprated driver. In...order for ETF to be cost effective, the accelerator system must be able to drive several target chambers which will test various Inertial Fusion ... Energy (IFE) reactor technologies. We envision an elevator system positioning and removing multiple target chambers from the center area of the ion beam

Cross-linking of the electron-transfer flavoprotein to electron-transfer flavoprotein-ubiquinone oxidoreductase with heterobifunctional reagents.

PubMed Central

Steenkamp, D J

1988-01-01

The mitochondrial electron-transfer flavoprotein (ETF) is a heterodimer containing only one FAD. In previous work on the structure-function relationships of ETF, its interaction with the general acyl-CoA dehydrogenase (GAD) was studied by chemical cross-linking with heterobifunctional reagents [D. J. Steenkamp (1987) Biochem. J. 243, 519-524]. GAD whose lysine residues were substituted with 3-(2-pyridyldithio)propionyl groups was preferentially cross-linked to the small subunit of ETF, the lysine residues of which had been substituted with 4-mercaptobutyramidine (MBA) groups. This work was extended to the interaction of ETF with ETF-ubiquinone oxidoreductase (ETF-Q ox). ETF-Q ox was partially inactivated by modification with N-succinimidyl 3-(2-pyridyldithio)propionate to introduce pyridyl disulphide structures. A similar modification of ETF caused a large increase in the apparent Michaelis constant of ETF-Q ox for modified ETF owing to the loss of positive charge on some critical lysines of ETF. When ETF-Q ox was modified with 2-iminothiolane to introduce 4-mercaptobutyramidine groups, only a minor effect on the activity of the enzyme was observed. To retain the positive charges on the lysine residues of ETF, pyridyl disulphide structures were introduced by treating ETF with 2-iminothiolane in the presence of 2,2'-dithiodipyridyl. The electron-transfer activity of the resultant ETF preparation containing 4-(2-pyridyldithio)butyramidine (PDBA) groups was only slightly affected. When ETF-Q ox substituted with MBA groups was mixed with ETF bearing PDBA groups, at least 70% of the cross-links formed between the two proteins were between the small subunit of ETF and ETF-Q ox. ETF-Q ox, therefore, interacts predominantly with the same subunit of ETF as GAD. Variables which affect the selectivity of ETF-Q ox cross-linking to the subunits of ETF are considered. Images Fig. 4. Fig. 5. Fig. 6. PMID:3145738

Site correction of a high-frequency strong-ground-motion simulation based on an empirical transfer function

NASA Astrophysics Data System (ADS)

Huang, Jyun-Yan; Wen, Kuo-Liang; Lin, Che-Min; Kuo, Chun-Hsiang; Chen, Chun-Te; Chang, Shuen-Chiang

2017-05-01

In this study, an empirical transfer function (ETF), which is the spectrum difference in Fourier amplitude spectra between observed strong ground motion and synthetic motion obtained by a stochastic point-source simulation technique, is constructed for the Taipei Basin, Taiwan. The basis stochastic point-source simulations can be treated as reference rock site conditions in order to consider site effects. The parameters of the stochastic point-source approach related to source and path effects are collected from previous well-verified studies. A database of shallow, small-magnitude earthquakes is selected to construct the ETFs so that the point-source approach for synthetic motions might be more widely applicable. The high-frequency synthetic motion obtained from the ETF procedure is site-corrected in the strong site-response area of the Taipei Basin. The site-response characteristics of the ETF show similar responses as in previous studies, which indicates that the base synthetic model is suitable for the reference rock conditions in the Taipei Basin. The dominant frequency contour corresponds to the shape of the bottom of the geological basement (the top of the Tertiary period), which is the Sungshan formation. Two clear high-amplification areas are identified in the deepest region of the Sungshan formation, as shown by an amplification contour of 0.5 Hz. Meanwhile, a high-amplification area was shifted to the basin's edge, as shown by an amplification contour of 2.0 Hz. Three target earthquakes with different kinds of source conditions, including shallow small-magnitude events, shallow and relatively large-magnitude events, and deep small-magnitude events relative to the ETF database, are tested to verify site correction. The results indicate that ETF-based site correction is effective for shallow earthquakes, even those with higher magnitudes, but is not suitable for deep earthquakes. Finally, one of the most significant shallow large-magnitude earthquakes (the 1999 Chi-Chi earthquake in Taiwan) is verified in this study. A finite fault stochastic simulation technique is applied, owing to the complexity of the fault rupture process for the Chi-Chi earthquake, and the ETF-based site-correction function is multiplied to obtain a precise simulation of high-frequency (up to 10 Hz) strong motions. The high-frequency prediction has good agreement in both time and frequency domain in this study, and the prediction level is the same as that predicted by the site-corrected ground motion prediction equation.

77 FR 9275 - Self-Regulatory Organizations; Chicago Board Options Exchange, Incorporated; Notice of Filing and...

Federal Register 2010, 2011, 2012, 2013, 2014

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... ETF Volatility Index, the CBOE Brazil ETF Volatility Index and CBOE Oil ETF Volatility Index February... Schedule to establish fees for transactions in options on the CBOE Emerging Market ETF Volatility Index (``VXEEM''), the CBOE Brazil ETF Volatility Index (``VXEWZ'') and the CBOE Crude Oil ETF Volatility Index...

242-A Evaporator quality assurance plan. Revision 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Basra, T.S.

1995-05-04

The purpose of this quality assurance project plan (Plan) is to provide requirements for activities pertaining to sampling, shipping, and analyses associated with candidate feed tank samples for the 242-A Evaporator project. The purpose of the 242-A Evaporator project is to reduce the volume of aqueous waste in the Double Shell Tank (DST) System and will result in considerable savings to the disposal of mixed waste. The 242-A Evaporator feed stream originates from DSTs identified as candidate feed tanks. The 242-A Evaporator reduces the volume of aqueous waste contained in DSTs by boiling off water and sending the condensate (calledmore » process condensate) to the Liquid Effluent Retention Facility (LEPF) storage basin where it is stored prior to treatment in the Effluent Treatment Facility (ETF). The objective of this quality assurance project plan is to provide the planning, implementation, and assessment of sample collection and analysis, data issuance, and validation activities for the candidate feed tanks.« less

77 FR 25218 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing of Proposed Rule Change To List...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-27

... Huntington US Equity Rotation Strategy ETF and Huntington EcoLogical Strategy ETF Under NYSE Arca Equities...''): Huntington US Equity Rotation Strategy ETF and Huntington EcoLogical Strategy ETF. The text of the proposed... Managed Fund Shares: \\3\\ Huntington US Equity Rotation Strategy ETF and Huntington EcoLogical Strategy ETF...

Nuclear factor ETF specifically stimulates transcription from promoters without a TATA box.

PubMed

Kageyama, R; Merlino, G T; Pastan, I

1989-09-15

Transcription factor ETF stimulates the expression of the epidermal growth factor receptor (EGFR) gene which does not have a TATA box in the promoter region. Here, we show that ETF recognizes various GC-rich sequences including stretches of deoxycytidine or deoxyguanosine residues and GC boxes with similar affinities. ETF also binds to TATA boxes but with a lower affinity. ETF stimulated in vitro transcription from several promoters without TATA boxes but had little or no effect on TATA box-containing promoters even though they had strong ETF-binding sites. These inactive ETF-binding sites became functional when placed upstream of the EGFR promoter whose own ETF-binding sites were removed. Furthermore, when a TATA box was introduced into the EGFR promoter, the responsiveness to ETF was abolished. These results indicate that ETF is a specific transcription factor for promoters which do not contain TATA elements.

Probing the dynamic interface between trimethylamine dehydrogenase (TMADH) and electron transferring flavoprotein (ETF) in the TMADH-2ETF complex: role of the Arg-alpha237 (ETF) and Tyr-442 (TMADH) residue pair.

PubMed

Burgess, Selena G; Messiha, Hanan Latif; Katona, Gergely; Rigby, Stephen E J; Leys, David; Scrutton, Nigel S

2008-05-06

We have used multiple solution state techniques and crystallographic analysis to investigate the importance of a putative transient interaction formed between Arg-alpha237 in electron transferring flavoprotein (ETF) and Tyr-442 in trimethylamine dehydrogenase (TMADH) in complex assembly, electron transfer, and structural imprinting of ETF by TMADH. We have isolated four mutant forms of ETF altered in the identity of the residue at position 237 (alphaR237A, alphaR237K, alphaR237C, and alphaR237E) and with each form studied electron transfer from TMADH to ETF, investigated the reduction potentials of the bound ETF cofactor, and analyzed complex formation. We show that mutation of Arg-alpha237 substantially destabilizes the semiquinone couple of the bound FAD and impedes electron transfer from TMADH to ETF. Crystallographic structures of the mutant ETF proteins indicate that mutation does not perturb the overall structure of ETF, but leads to disruption of an electrostatic network at an ETF domain boundary that likely affects the dynamic properties of ETF in the crystal and in solution. We show that Arg-alpha237 is required for TMADH to structurally imprint the as-purified semiquinone form of wild-type ETF and that the ability of TMADH to facilitate this structural reorganization is lost following (i) redox cycling of ETF, or simple conversion to the oxidized form, and (ii) mutagenesis of Arg-alpha237. We discuss this result in light of recent apparent conflict in the literature relating to the structural imprinting of wild-type ETF. Our studies support a mechanism of electron transfer by conformational sampling as advanced from our previous analysis of the crystal structure of the TMADH-2ETF complex [Leys, D. , Basran, J. , Sutcliffe, M. J., and Scrutton, N. S. (2003) Nature Struct. Biol. 10, 219-225] and point to a key role for the Tyr-442 (TMADH) and Arg-alpha237 (ETF) residue pair in transiently stabilizing productive electron transfer configurations. Our work also points to the importance of Arg-alpha237 in controlling the thermodynamics of electron transfer, the dynamics of ETF, and the protection of reducing equivalents following disassembly of the TMADH-2ETF complex.

A study on the morphology of polystyrene-grafted poly(ethylene-alt-tetrafluoroethylene) (ETFE) films prepared using a simultaneous radiation grafting method

NASA Astrophysics Data System (ADS)

Song, Ju-Myung; Ko, Beom-Seok; Sohn, Joon-Yong; Nho, Young Chang; Shin, Junhwa

2014-04-01

The morphology of polystyrene-grafted poly(ethylene-alt-tetrafluoroethylene) (ETFE) films prepared using a simultaneous radiation grafting method was investigated using DMA, DSC, XRD, and SAXS instruments. The DMA study indicates that the ETFE amorphous phase and PS amorphous phase are mixed well in the PS-grafted ETFE films while the ETFE crystalline phase and the PS amorphous phase are separated, suggesting that the PS chains are grafted mainly on the ETFE amorphous regions. The DSC and XRD data showed that the natural crystalline structures of ETFE in the grafted ETFE films are not affected by the degree of grafting. The SAXS profiles displayed that the inter-crystalline distance of the ETFE films increases with an increasing degree of grafting, which further implies that the PS graft chains formed by the simultaneous irradiation has a significant impact on the amorphous morphology of the resulting grafted ETFE film. Thus, these results indicate that the styrene monomers are mainly grafted on the ETFE amorphous regions during the simultaneous radiation grafting process.

76 FR 28493 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing of Proposed Rule Change To List...

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... of ProShares Short VIX Short-Term Futures ETF, ProShares Short VIX Mid-Term Futures ETF, ProShares Ultra VIX Short-Term Futures ETF, ProShares Ultra VIX Mid- Term Futures ETF, ProShares UltraShort VIX Short-Term Futures ETF, and ProShares UltraShort VIX Mid-Term Futures ETF Under NYSE Arca Equities Rule...

Formation of W(3)A(1) electron-transferring flavoprotein (ETF) hydroquinone in the trimethylamine dehydrogenase x ETF protein complex.

PubMed

Jang, M H; Scrutton, N S; Hille, R

2000-04-28

The electron-transferring flavoprotein (ETF) from Methylophilus methylotrophus (sp. W(3)A(1)) exhibits unusual oxidation-reduction properties and can only be reduced to the level of the semiquinone under most circumstances (including turnover with its physiological reductant, trimethylamine dehydrogenase (TMADH), or reaction with strong reducing reagents such as sodium dithionite). In the present study, we demonstrate that ETF can be reduced fully to its hydroquinone form both enzymatically and chemically when it is in complex with TMADH. Quantitative titration of the TMADH x ETF protein complex with sodium dithionite shows that a total of five electrons are taken up by the system, indicating that full reduction of ETF occurs within the complex. The results indicate that the oxidation-reduction properties of ETF are perturbed upon binding to TMADH, a conclusion further supported by the observation of a spectral change upon formation of the TMADH x ETF complex that is due to a change in the environment of the FAD of ETF. The results are discussed in the context of ETF undergoing a conformational change during formation of the TMADH x ETF electron transfer complex, which modulates the spectral and oxidation-reduction properties of ETF such that full reduction of the protein can take place.

Binding of the human "electron transferring flavoprotein" (ETF) to the medium chain acyl-CoA dehydrogenase (MCAD) involves an arginine and histidine residue.

PubMed

Parker, Antony R

2003-10-01

The interaction between the "electron transferring flavoprotein" (ETF) and medium chain acyl-CoA dehydrogenase (MCAD) enables successful flavin to flavin electron transfer, crucial for the beta-oxidation of fatty acids. The exact biochemical determinants for ETF binding to MCAD are unknown. Here we show that binding of human ETF, to MCAD, was inhibited by 2,3-butanedione and diethylpyrocarbonate (DEPC) and reversed by incubation with free arginine and hydroxylamine respectively. Spectral analyses of native ETF vs modified ETF suggested that flavin binding was not affected and that the loss of ETF activity with MCAD involved modification of one ETF arginine residue and one ETF histidine residue respectively. MCAD and octanoyl-CoA protected ETF against inactivation by both 2,3-butanedione and DEPC indicating that the arginine and histidine residues are present in or around the MCAD binding site. Comparison of exposed arginine and histidine residues among different ETF species, however, indicates that arginine residues are highly conserved but that histidine residues are not. These results lead us to conclude that this single arginine residue is essential for the binding of ETF to MCAD, but that the single histidine residue, although involved, is not.

Defining redox centers in human electron transfer flavoprotein: ubiquinone oxidoreductase (ETF:QO) by expression in Saccharomyces cerevisiae

DOE Office of Scientific and Technical Information (OSTI.GOV)

Frerman, F.E.; Beard, S.; Goodman, S.I.

Mutations in ETF or ETC:QO cause glutaric acidemia type II (GA2). ETF:QO is an iron-sulfur flavoprotein in the inner mitochondrial membrane which transfers electrons from ETF in the mitochondrial matrix to ubiquinone (Q). The human ETF:QO gene is on chromosome 4q32{r_arrow}qter, and encodes a 617 amino acid precursor which is processed to the 64 kDa mature form in the mitochondrion. One ETF:QO mutation in GA2 is a G{r_arrow}T transversion in a donor splice site, deleting the 222 bp upstream exon from the transcript. The deleted 74 amino acids are near the carboxyl terminus just beyond a predicted membrane helix, andmore » include C561, one of four cysteine residues predicted to ligate the 4Fe4S cluster. The mutant protein is not stable in patient fibroblasts. We have expressed cDNAs encoding wild type (wt) ETF:QO, ETF:QO with the 74 amino acid deletion, and ETFF:QO with only a C561A mutation, in S cerevisiae. In all instances, precursor and mature ETF:QOs were stably inserted into the mitochondrial membrane. ETF:QO (C561A) is extracted from the membrane under the same conditions as wt ETF:QO, but ETF:QO with the deletion is much more difficult to extract. Wt ETF:QO accepts electrons from ETF and reduces Q but, while both mutant proteins accept electrons from ETF, neither of them reduces Q. This work demonstrates that C561 in human ETF:QO is essential for Q reduction (probably because it ligands the 4Fe4S cluster), that mutant proteins that are unstable in man may be stable in other systems, that cleavage of signal peptide from precursor proteins can occur within the inner mitochondrial membrane, and the general usefulness of expressing human mitochondrial proteins in yeast.« less

78 FR 39793 - ETF Issuer Solutions Inc., et al.; Notice of Application

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... SECURITIES AND EXCHANGE COMMISSION [Investment Company Act Release No. 30568; 812-14080] ETF... sections 12(d)(1)(A) and (B) of the Act. Applicants: ETF Issuer Solutions Inc. (``ETFis''), ETF Actively Managed Trust (``Trust) and ETF Distributors LLC (``Distributor''). Summary of Application: Applicants...

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... measure the relative total returns of a stock or exchange-traded fund (``ETF'') against another stock or ETF, including where one of the reference ETFs measured by the index is a gold- or silver-based ETF.\\4... reference securities of an underlying relative performance index is an ETF designed to measure the return of...

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... individual underlying stocks) that comprise a particular ETF, HOLDR or index. Any such proposal would be the... Exchange would identify the particular underlying security--or with respect to ETF(s), HOLDRS(s), and index... include related ETF(s), HOLDRS(s), and/or index value(s),\\9\\ and/or related futures product(s),\\10\\ and...

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76 FR 62891 - Self-Regulatory Organizations; Options Clearing Corporation; Notice of Filing of Proposed Rule...

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2011-10-11

... relative total returns of a stock or exchange-traded fund (``ETF'') against another stock or ETF, including where one of the reference ETFs measured by the index is a gold- or silver-based ETF.\\3\\ Generally, a... of an underlying relative performance index is an ETF designed to measure the return of gold or...

The effect of enteral tube feeding in cystic fibrosis: A registry based study.

PubMed

Libeert, Denis; Declercq, Dimitri; Wanyama, Simeon; Thomas, Muriel; Van Daele, Sabine; De Baets, Frans; Van Biervliet, Stephanie

2018-03-01

Long-term effect of enteral tube feeding (ETF) in cystic fibrosis (CF) remains equivocal. A Belgian CF registry based, retrospective, longitudinal study, evaluated the pre- and post- ETF (n = 113) clinical evolution and compared each patient with 2 age, gender, pancreatic status and genotype class-matched controls. At baseline ETF had a worse BMI z-score (p < 0.0001) and FEV1% (p < 0.0001) compared to controls. Patients eventually receiving ETF, had already a significant worse nutritional status and pulmonary function at first entry in the registry. Both parameters displayed a significant decline before ETF-introduction. ETF had more hospitalization and intravenous antibiotic (IVAB) treatment days (p < 0.0001). After ETF introduction hospitalizations and IVAB decreased significantly. After ETF-introduction BMI z-score recuperated towards the original curve before the decline, but remained below the controls. Starting ETF had no effect on rate of height gain in children. The pre-index FEV1 decline (-1.52%/year (p = 0.002)) stabilized to +0.39%/year afterwards. Controls displayed decline of -0.48%/year (p < 0.0001). ETF introduction improved BMI z-score and stabilized FEV1, associated with less hospitalizations and IVAB treatments. Higher mortality and transplantation in the ETF cases, leading to drop-outs, made determination of the effect size difficult. Copyright © 2018 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

Electron transfer flavoprotein deficiency: functional and molecular aspects.

PubMed

Schiff, Manuel; Froissart, Roseline; Olsen, Rikke K J; Acquaviva, Cécile; Vianey-Saban, Christine

2006-06-01

Multiple acyl-CoA dehydrogenase deficiency (MADD) is a recessively inherited metabolic disorder that can be due to a deficiency of electron transfer flavoprotein (ETF) or its dehydrogenase (ETF-ubiquinone oxidoreductase). ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. In the present study, we have analysed tissue samples from 16 unrelated patients with ETF deficiency, and we report the results of ETF activity, Western blot analysis and mutation analysis. The ETF assay provides a reliable diagnostic tool to confirm ETF deficiency in patients suspected to suffer from MADD. Activity ranged from less than 1 to 16% of controls with the most severely affected patients disclosing the lowest activity values. The majority of patients had mutations in the ETFA gene while only two of them harboured mutations in the ETFB gene. Nine novel disease-causing ETF mutations are reported.

Phylogenetic characterization of the ubiquitous electron transfer flavoprotein families ETF-alpha and ETF-beta.

PubMed

Tsai, M H; Saier, M H

1995-06-01

Electron transfer flavoproteins (ETF) are alpha beta-heterodimers found in eukaryotic mitochondria and bacteria. We have identified currently sequenced protein members of the ETF-alpha and ETF-beta families. Members of these two families include (a) the ETF subunits of mammals and bacteria, (b) homologous pairs of proteins (FixB/FixA) that are essential for nitrogen fixation in some bacteria, and (c) a pair of carnitine-inducible proteins encoded by two open reading frames in Escherichia coli (YaaQ and YaaR). These three groups of proteins comprise three clusters on both the ETF-alpha and ETF-beta phylogenetic trees, separated from each other by comparable phylogenetic distances. This fact suggests that these two protein families evolved with similar overall rates of evolutionary divergence. Relative regions of sequence conservation are evaluated, and signature sequences for both families are derived.

RESULTS OF TRITIUM TRACKING AND GROUNDWATER MONITORING AT THE HANFORD SITE 200 AREA STATE APPROVED LAND DISPOSAL SITE FY2008

DOE Office of Scientific and Technical Information (OSTI.GOV)

ERB DB

2008-11-19

The Hanford Site's 200 Area Effluent Treatment Facility (ETF) processes contaminated aqueous wastes derived from Hanford Site facilities. The treated wastewater occasionally contains tritium, which cannot be removed by the ETF prior to the wastewater being discharged to the 200 Area State-Approved Land Disposal Site (SALDS). During the first 11 months of fiscal year 2008 (FY08) (September 1, 2007, to July 31, 2008), approximately 75.15 million L (19.85 million gal) of water were discharged to the SALDS. Groundwater monitoring for tritium and other constituents, as well as water-level measurements, is required for the SALDS by State Waste Discharge Permit Numbermore » ST-4500 (Ecology 2000). The current monitoring network consists of three proximal (compliance) monitoring wells and nine tritium-tracking wells. Quarterly sampling of the proximal wells occurred in October 2007 and in January/February 2008, April 2008, and August 2008. The nine tritium-tracking wells, including groundwater monitoring wells located upgradient and downgradient of the SALDS, were sampled in January through April 2008. Water-level measurements taken in the three proximal SALDS wells indicate that a small groundwater mound is present beneath the facility, which is a result of operational discharges. The mound increased in FY08 due to increased ETF discharges from treating groundwater from extraction wells at the 200-UP-l Operable Unit and the 241-T Tank Farm. Maximum tritium activities increased by an order of magnitude at well 699-48-77A (to 820,000 pCi/L in April 2008) but remained unchanged in the other two proximal wells. The increase was due to higher quantities of tritium in wastewaters that were treated and discharged in FY07 beginning to appear at the proximal wells. The FY08 tritium activities for the other two proximal wells were 68,000 pCi/L at well 699-48-77C (October 2007) and 120,000 pCi/L at well 699-48-77D (October 2007). To date, no indications of a tritium incursion from the SALDS have been detected in the tritium-tracking wells. Concentrations of all chemical constituents were within Permit limits or were below method detection limits when sampled during FY08. A summary of the chemical constituent concentrations or method detection limits is provided in Table 3-2 in the main text discussion. This report presents the results of groundwater monitoring and tritium-tracking samples from the SALDS facility during FY08. Due to the 30-day laboratory turnaround for analysis of proximal well groundwater samples, this report addresses available date extending from August 1, 2007, through September 30, 2008 (August 2007 data were not included in the FY07 report). Updated background information, which is necessary to understand the results of the groundwater analyses, is also provided on facility operations. Interpretive discussions and recommendations for future monitoring are also provided, where possible.« less

Stabilization of non-productive conformations underpins rapid electron transfer to electron-transferring flavoprotein.

PubMed

Toogood, Helen S; van Thiel, Adam; Scrutton, Nigel S; Leys, David

2005-08-26

Crystal structures of protein complexes with electron-transferring flavoprotein (ETF) have revealed a dual protein-protein interface with one region serving as anchor while the ETF FAD domain samples available space within the complex. We show that mutation of the conserved Glu-165beta in human ETF leads to drastically modulated rates of interprotein electron transfer with both medium chain acyl-CoA dehydrogenase and dimethylglycine dehydrogenase. The crystal structure of free E165betaA ETF is essentially identical to that of wild-type ETF, but the crystal structure of the E165betaA ETF.medium chain acyl-CoA dehydrogenase complex reveals clear electron density for the FAD domain in a position optimal for fast interprotein electron transfer. Based on our observations, we present a dynamic multistate model for conformational sampling that for the wild-type ETF. medium chain acyl-CoA dehydrogenase complex involves random motion between three distinct positions for the ETF FAD domain. ETF Glu-165beta plays a key role in stabilizing positions incompatible with fast interprotein electron transfer, thus ensuring high rates of complex dissociation.

Glutaric acidemia type II: gene structure and mutations of the electron transfer flavoprotein:ubiquinone oxidoreductase (ETF:QO) gene.

PubMed

Goodman, Stephen I; Binard, Robert J; Woontner, Michael R; Frerman, Frank E

2002-01-01

Glutaric acidemia type II is a human inborn error of metabolism which can be due to defects in either subunit of electron transfer flavoprotein (ETF) or in ETF:ubiquinone oxidoreductase (ETF:QO), but few disease-causing mutations have been described. The ETF:QO gene is located on 4q33, and contains 13 exons. Primers to amplify these exons are presented, together with mutations identified by molecular analysis of 20 ETF:QO-deficient patients. Twenty-one different disease-causing mutations were identified on 36 of the 40 chromosomes.

Evaluation of the electron transfer flavoprotein as an antibacterial target in Burkholderia cenocepacia.

PubMed

Stietz, Maria S; Lopez, Christina; Osifo, Osasumwen; Tolmasky, Marcelo E; Cardona, Silvia T

2017-10-01

There are hundreds of essential genes in multidrug-resistant bacterial genomes, but only a few of their products are exploited as antibacterial targets. An example is the electron transfer flavoprotein (ETF), which is required for growth and viability in Burkholderia cenocepacia. Here, we evaluated ETF as an antibiotic target for Burkholderia cepacia complex (Bcc). Depletion of the bacterial ETF during infection of Caenorhabditis elegans significantly extended survival of the nematodes, proving that ETF is essential for survival of B. cenocepacia in this host model. In spite of the arrest in respiration in ETF mutants, the inhibition of etf expression did not increase the formation of persister cells, when treated with high doses of ciprofloxacin or meropenem. To test if etf translation could be inhibited by RNA interference, antisense oligonucleotides that target the etfBA operon were synthesized. One antisense oligonucleotide was effective in inhibiting etfB translation in vitro but not in vivo, highlighting the challenge of reduced membrane permeability for the design of drugs against B. cenocepacia. This work contributes to the validation of ETF of B. cenocepacia as a target for antibacterial therapy and demonstrates the utility of a C. elegans liquid killing assay to validate gene essentiality in an in vivo infection model.

alphaT244M mutation affects the redox, kinetic, and in vitro folding properties of Paracoccus denitrificans electron transfer flavoprotein.

PubMed

Griffin, K J; Dwyer, T M; Manning, M C; Meyer, J D; Carpenter, J F; Frerman, F E

1997-04-08

Threonine 244 in the alpha subunit of Paracoccus denitrificans transfer flavoprotein (ETF) lies seven residues to the amino terminus of a proposed dinucleotide binding motif for the ADP moiety of the FAD prosthetic group. This residue is highly conserved in the alpha subunits of all known ETFs, and the most frequent pathogenic mutation in human ETF encodes a methionine substitution at the corresponding position, alphaT266. The X-ray crystal structures of human and P. denitrificans ETFs are very similar. The hydroxyl hydrogen and a backbone amide hydrogen of alphaT266 are hydrogen bonded to N(5) and C(4)O of the flavin, respectively, and the corresponding alphaT244 has the same structural role in P. denitrificans ETF. We substituted a methionine for T244 in the alpha subunit of P. denitrificans ETF and expressed the mutant ETF in Escherichia coli. The mutant protein was purified, characterized, and compared with wild type P. denitrificans ETF. The mutation has no significant effect on the global structure of the protein as inferred from visible and near-ultraviolet absorption and circular dichroism spectra, far-ultraviolet circular dichroism spectra, and infrared spectra in 1H2O and 2H2O. Intrinsic fluorescence due to tryptophan of the mutant protein is 60% greater than that of the wild type ETF. This increased tryptophan fluorescence is probably due to a change in the environment of the nearby W239. Tyrosine fluorescence is unchanged in the mutant protein, although two tyrosine residues are close to the site of the mutation. These results indicate that a change in structure is minor and localized. Kinetic constants of the reductive half-reaction of ETF with porcine medium chain acyl-CoA dehydrogenase are unaltered when alphaT244M ETF serves as the substrate; however, the mutant ETF fails to exhibit saturation kinetics when the semiquinone form of the protein is used as the substrate in the disproportionation reaction catalyzed by P. denitrificans electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO). The redox behavior of the mutant ETF was also altered as determined from the equilibrium constant of the disproportionation reaction. The separation of flavin redox potentials between the oxidized/semiquinone couple and semiquinone/hydroquinone couple are -6 mV in the wild type ETF and -27 mV in the mutant ETF. The mutation does not alter the AMP content of the protein, although the extent and fidelity of AMP-dependent, in vitro renaturation of the mutant AMP-free apoETF is reduced by 57% compared to renaturation of wild type apoETF, likely due to the absence of the potential hydrogen bond donor T244.

Molecular mechanisms of riboflavin responsiveness in patients with ETF-QO variations and multiple acyl-CoA dehydrogenation deficiency.

PubMed

Cornelius, Nanna; Frerman, Frank E; Corydon, Thomas J; Palmfeldt, Johan; Bross, Peter; Gregersen, Niels; Olsen, Rikke K J

2012-08-01

Riboflavin-responsive forms of multiple acyl-CoA dehydrogenation deficiency (RR-MADD) have been known for years, but with presumed defects in the formation of the flavin adenine dinucleotide (FAD) co-factor rather than genetic defects of electron transfer flavoprotein (ETF) or electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO). It was only recently established that a number of RR-MADD patients carry genetic defects in ETF-QO and that the well-documented clinical efficacy of riboflavin treatment may be based on a chaperone effect that can compensate for inherited folding defects of ETF-QO. In the present study, we investigate the molecular mechanisms and the genotype-phenotype relationships for the riboflavin responsiveness in MADD, using a human HEK-293 cell expression system. We studied the influence of riboflavin and temperature on the steady-state level and the activity of variant ETF-QO proteins identified in patients with RR-MADD, or non- and partially responsive MADD. Our results showed that variant ETF-QO proteins associated with non- and partially responsive MADD caused severe misfolding of ETF-QO variant proteins when cultured in media with supplemented concentrations of riboflavin. In contrast, variant ETF-QO proteins associated with RR-MADD caused milder folding defects when cultured at the same conditions. Decreased thermal stability of the variants showed that FAD does not completely correct the structural defects induced by the variation. This may cause leakage of electrons and increased reactive oxygen species, as reflected by increased amounts of cellular peroxide production in HEK-293 cells expressing the variant ETF-QO proteins. Finally, we found indications of prolonged association of variant ETF-QO protein with the Hsp60 chaperonin in the mitochondrial matrix, supporting indications of folding defects in the variant ETF-QO proteins.

Embryotrophic factor-3 from human oviductal cells affects the messenger RNA expression of mouse blastocyst.

PubMed

Lee, Y L; Lee, K F; Xu, J S; Kwok, K L; Luk, J M; Lee, W M; Yeung, W S B

2003-02-01

Our previous results showed that embryotrophic factor-3 (ETF-3) from human oviductal cells increased the size and hatching rate of mouse blastocysts in vitro. The present study investigated the production of ETF-3 by an immortalized human oviductal cell line (OE-E6/E7) and the effects of ETF-3 on the mRNA expression of mouse embryos. The ETF-3 was purified from primary oviductal cell conditioned media using sequential liquid chromatographic systems, and antiserum against ETF-3 was raised. The ETF-3-supplemented Chatot-Ziomek-Bavister medium was used to culture Day 1 MF1 x BALB/c mouse embryos for 4 days. The ETF-3 treatment significantly enhanced the mouse embryo blastulation and hatching rate. The antiserum, at concentrations of 0.03-3%, abolished the embryotrophic effect of ETF-3. Positive ETF-3 immunoreactivity was detected in the primary oviductal cells, OE-E6/E7, and blastocysts derived from ETF-3 treatment. Vero cells (African Green Monkey kidney cell line), fibroblasts, and embryos cultured in control medium did not possess ETF-3 immunoreactivity. The mRNA expression patterns of the treated embryos were studied at the blastocyst stage by mRNA differential display reverse transcription-polymerase chain reaction (DDRT-PCR). The DDRT-PCR showed that some of the mRNAs were differentially expressed after ETF-3 treatment. Twelve of the differentially expressed mRNAs that had high homology with cDNA sequences in the GenBank were selected for further characterization. The differential expression of seven of these mRNAs (ezrin, heat shock 70-kDa protein, cytochrome c oxidase subunit VIIa-L precursor, proteinase-activated receptor 2, eukaryotic translation initiation factor 2beta, cullin 1, and proliferating cell nuclear antigen) was confirmed by semiquantitative RT-PCR. In conclusion, immortalized oviductal cells produce ETF-3, which influences mRNA expression of mouse blastocyst.

A multifractal detrended fluctuation analysis of financial market efficiency: Comparison using Dow Jones sector ETF indices

NASA Astrophysics Data System (ADS)

Tiwari, Aviral Kumar; Albulescu, Claudiu Tiberiu; Yoon, Seong-Min

2017-10-01

This study challenges the efficient market hypothesis, relying on the Dow Jones sector Exchange-Traded Fund (ETF) indices. For this purpose, we use the generalized Hurst exponent and multifractal detrended fluctuation analysis (MF-DFA) methods, using daily data over the timespan from 2000 to 2015. We compare the sector ETF indices in terms of market efficiency between short- and long-run horizons, small and large fluctuations, and before and after the global financial crisis (GFC). Our findings can be summarized as follows. First, there is clear evidence that the sector ETF markets are multifractal in nature. We also find a crossover in the multifractality of sector ETF market dynamics. Second, the utilities and consumer goods sector ETF markets are more efficient compared with the financial and telecommunications sector ETF markets, in terms of price prediction. Third, there are noteworthy discrepancies in terms of market efficiency, between the short- and long-term horizons. Fourth, the ETF market efficiency is considerably diminished after the global financial crisis.

Preferential cross-linking of the small subunit of the electron-transfer flavoprotein to general acyl-CoA dehydrogenase.

PubMed Central

Steenkamp, D J

1987-01-01

The interaction between pig liver mitochondrial electron-transfer flavoprotein (ETF) and general acyl-CoA dehydrogenase (GAD) was investigated by means of the heterobifunctional reagent N-succinimidyl 3-(2-pyridyldithio)propionate. Neither ETF or GAD contained reactive thiol groups. The substitution of 9.4 lysine residues/FAD group in GAD with pyridyl disulphide structures did not affect the catalytic activity of the enzyme. Thiol groups were introduced into ETF by thiolation with methyl 4-mercaptobutyrimidate. ETF containing 10.5 reactive thiol groups/FAD group showed undiminished electron-acceptor activity with respect to GAD. The reaction of thiolated ETF and GAD containing pyridyl disulphide structures resulted in a decreased staining intensity of the small subunit of ETF on SDS/polyacrylamide-gel electrophoresis. Preferential cross-linking of the smaller subunit of ETF to GAD did not take place when ETF was first treated with SDS, but was unaffected by reduction of GAD by octanoyl-CoA. Images Fig. 2. Fig. 3. Fig. 5. PMID:3115254

Defining Electron Bifurcation in the Electron-Transferring Flavoprotein Family.

PubMed

Garcia Costas, Amaya M; Poudel, Saroj; Miller, Anne-Frances; Schut, Gerrit J; Ledbetter, Rhesa N; Fixen, Kathryn R; Seefeldt, Lance C; Adams, Michael W W; Harwood, Caroline S; Boyd, Eric S; Peters, John W

2017-11-01

Electron bifurcation is the coupling of exergonic and endergonic redox reactions to simultaneously generate (or utilize) low- and high-potential electrons. It is the third recognized form of energy conservation in biology and was recently described for select electron-transferring flavoproteins (Etfs). Etfs are flavin-containing heterodimers best known for donating electrons derived from fatty acid and amino acid oxidation to an electron transfer respiratory chain via Etf-quinone oxidoreductase. Canonical examples contain a flavin adenine dinucleotide (FAD) that is involved in electron transfer, as well as a non-redox-active AMP. However, Etfs demonstrated to bifurcate electrons contain a second FAD in place of the AMP. To expand our understanding of the functional variety and metabolic significance of Etfs and to identify amino acid sequence motifs that potentially enable electron bifurcation, we compiled 1,314 Etf protein sequences from genome sequence databases and subjected them to informatic and structural analyses. Etfs were identified in diverse archaea and bacteria, and they clustered into five distinct well-supported groups, based on their amino acid sequences. Gene neighborhood analyses indicated that these Etf group designations largely correspond to putative differences in functionality. Etfs with the demonstrated ability to bifurcate were found to form one group, suggesting that distinct conserved amino acid sequence motifs enable this capability. Indeed, structural modeling and sequence alignments revealed that identifying residues occur in the NADH- and FAD-binding regions of bifurcating Etfs. Collectively, a new classification scheme for Etf proteins that delineates putative bifurcating versus nonbifurcating members is presented and suggests that Etf-mediated bifurcation is associated with surprisingly diverse enzymes. IMPORTANCE Electron bifurcation has recently been recognized as an electron transfer mechanism used by microorganisms to maximize energy conservation. Bifurcating enzymes couple thermodynamically unfavorable reactions with thermodynamically favorable reactions in an overall spontaneous process. Here we show that the electron-transferring flavoprotein (Etf) enzyme family exhibits far greater diversity than previously recognized, and we provide a phylogenetic analysis that clearly delineates bifurcating versus nonbifurcating members of this family. Structural modeling of proteins within these groups reveals key differences between the bifurcating and nonbifurcating Etfs. Copyright © 2017 American Society for Microbiology.

Defining Electron Bifurcation in the Electron-Transferring Flavoprotein Family

PubMed Central

Garcia Costas, Amaya M.; Poudel, Saroj; Miller, Anne-Frances; Schut, Gerrit J.; Ledbetter, Rhesa N.; Seefeldt, Lance C.; Adams, Michael W. W.

2017-01-01

ABSTRACT Electron bifurcation is the coupling of exergonic and endergonic redox reactions to simultaneously generate (or utilize) low- and high-potential electrons. It is the third recognized form of energy conservation in biology and was recently described for select electron-transferring flavoproteins (Etfs). Etfs are flavin-containing heterodimers best known for donating electrons derived from fatty acid and amino acid oxidation to an electron transfer respiratory chain via Etf-quinone oxidoreductase. Canonical examples contain a flavin adenine dinucleotide (FAD) that is involved in electron transfer, as well as a non-redox-active AMP. However, Etfs demonstrated to bifurcate electrons contain a second FAD in place of the AMP. To expand our understanding of the functional variety and metabolic significance of Etfs and to identify amino acid sequence motifs that potentially enable electron bifurcation, we compiled 1,314 Etf protein sequences from genome sequence databases and subjected them to informatic and structural analyses. Etfs were identified in diverse archaea and bacteria, and they clustered into five distinct well-supported groups, based on their amino acid sequences. Gene neighborhood analyses indicated that these Etf group designations largely correspond to putative differences in functionality. Etfs with the demonstrated ability to bifurcate were found to form one group, suggesting that distinct conserved amino acid sequence motifs enable this capability. Indeed, structural modeling and sequence alignments revealed that identifying residues occur in the NADH- and FAD-binding regions of bifurcating Etfs. Collectively, a new classification scheme for Etf proteins that delineates putative bifurcating versus nonbifurcating members is presented and suggests that Etf-mediated bifurcation is associated with surprisingly diverse enzymes. IMPORTANCE Electron bifurcation has recently been recognized as an electron transfer mechanism used by microorganisms to maximize energy conservation. Bifurcating enzymes couple thermodynamically unfavorable reactions with thermodynamically favorable reactions in an overall spontaneous process. Here we show that the electron-transferring flavoprotein (Etf) enzyme family exhibits far greater diversity than previously recognized, and we provide a phylogenetic analysis that clearly delineates bifurcating versus nonbifurcating members of this family. Structural modeling of proteins within these groups reveals key differences between the bifurcating and nonbifurcating Etfs. PMID:28808132

Development of a CFRP Engine Thrust Frame for the Next Generation Launchers

NASA Astrophysics Data System (ADS)

Fatemi, Javad; van der Bas, Finn; Cruijssen, Henk

2012-07-01

This paper addresses the activities related to the development of technologies for a composite Engine Thrust Frame (ETF) for the next generation launchers. In particular, the design and analyses of a full Carbon Fibre Reinforced Plastic (CFRP) engine thrust frame are presented in more detail. The ETF concept is composed of three main parts, i.e. an aluminium top-ring which connects the ETF to the upper-stage tank, a CFRP cone, and a CFRP cone-cap which connects the Vinci engine to the ETF. The main challenging requirements for development of a CFRP ETF are recalled. The ETF concept and its mechanical performances are assessed.

77 FR 36022 - Notice of Application; Precidian ETFs Trust, et al.

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-15

... Application; Precidian ETFs Trust, et al. June 8, 2012. AGENCY: Securities and Exchange Commission...). \\1\\ Precidian ETFs Trust, Investment Company Act Release Nos. 29692 (June 9, 2011) (notice) and 29712 (July 1, 2011) (order). Applicants: Precidian ETFs Trust (``Trust''), Precidian Funds LLC (``Adviser...

Dynamics driving function: new insights from electron transferring flavoproteins and partner complexes.

PubMed

Toogood, Helen S; Leys, David; Scrutton, Nigel S

2007-11-01

Electron transferring flavoproteins (ETFs) are soluble heterodimeric FAD-containing proteins that function primarily as soluble electron carriers between various flavoprotein dehydrogenases. ETF is positioned at a key metabolic branch point, responsible for transferring electrons from up to 10 primary dehydrogenases to the membrane-bound respiratory chain. Clinical mutations of ETF result in the often fatal disease glutaric aciduria type II. Structural and biophysical studies of ETF in complex with partner proteins have shown that ETF partitions the functions of partner binding and electron transfer between (a) a 'recognition loop', which acts as a static anchor at the ETF-partner interface, and (b) a highly mobile redox-active FAD domain. Together, this enables the FAD domain of ETF to sample a range of conformations, some compatible with fast interprotein electron transfer. This 'conformational sampling' enables ETF to recognize structurally distinct partners, whilst also maintaining a degree of specificity. Complex formation triggers mobility of the FAD domain, an 'induced disorder' mechanism contrasting with the more generally accepted models of protein-protein interaction by induced fit mechanisms. We discuss the implications of the highly dynamic nature of ETFs in biological interprotein electron transfer. ETF complexes point to mechanisms of electron transfer in which 'dynamics drive function', a feature that is probably widespread in biology given the modular assembly and flexible nature of biological electron transfer systems.

Decomposition of the fluorescence spectra of two FAD molecules in electron-transferring flavoprotein from Megasphaera elsdenii.

PubMed

Sato, Kyosuke; Nishina, Yasuzo; Shiga, Kiyoshi

2013-07-01

Electron-transferring flavoprotein (ETF) from Megasphaera elsdenii contains two FAD molecules, FAD-1 and FAD-2. FAD-2 shows an unusual absorption spectrum with a 400-nm peak. In contrast, ETFs from other sources such as pig contain one FAD and one AMP with the FAD showing a typical flavin absorption spectrum with 380- and 440-nm peaks. It is presumed that FAD-2 is the counterpart of the FAD in other ETFs. In this study, the FAD-1 and FAD-2 fluorescence spectra were determined by titration of FAD-1-bound ETF with FAD using excitation-emission matrix (EEM) fluorescence spectroscopy. The EEM data were globally analysed, and the FAD fluorescence spectra were calculated from the principal components using their respective absorption spectra. The FAD-2 fluorescence spectrum was different from that of pig ETF, which is more intense and blue-shifted. AMP-free pig ETF in acidic solution, which has a comparable absorption spectrum to FAD-2, also had a similar fluorescence spectrum. This result suggests that FAD-2 in M. elsdenii ETF and the FAD in acidic AMP-free pig ETF share a common microenvironment. A review of published ETF fluorescence spectra led to the speculation that the majority of ETF molecules in solution are in the conformation depicted by the crystal structure.

Discrete hierarchy of sizes and performances in the exchange-traded fund universe

NASA Astrophysics Data System (ADS)

Vandermarliere, B.; Ryckebusch, J.; Schoors, K.; Cauwels, P.; Sornette, D.

2017-03-01

Using detailed statistical analyses of the size distribution of a universe of equity exchange-traded funds (ETFs), we discover a discrete hierarchy of sizes, which imprints a log-periodic structure on the probability distribution of ETF sizes that dominates the details of the asymptotic tail. This allows us to propose a classification of the studied universe of ETFs into seven size layers approximately organized according to a multiplicative ratio of 3.5 in their total market capitalization. Introducing a similarity metric generalizing the Herfindhal index, we find that the largest ETFs exhibit a significantly stronger intra-layer and inter-layer similarity compared with the smaller ETFs. Comparing the performance across the seven discerned ETF size layers, we find an inverse size effect, namely large ETFs perform significantly better than the small ones both in 2014 and 2015.

A single arginine residue is required for the interaction of the electron transferring flavoprotein (ETF) with three of its dehydrogenase partners.

PubMed

Parker, Antony R

2003-12-01

The interaction of several dehydrogenases with the electron transferring flavoprotein (ETF) is a crucial step required for the successful transfer of electrons into the electron transport chain. The exact determinants regarding the interaction of ETF with its dehydrogenase partners are still unknown. Chemical modification of ETF with arginine-specific reagents resulted in the loss, to varying degrees, of activity with medium chain acyl-coenzyme A dehydrogenase (MCAD). The kinetic profiles showed the inactivations followed pseudo-first-order kinetics for all reagents used. For activity with MCAD, maximum inactivation of ETF was accomplished by 2,3-butanedione (4% residual activity after 120 min) and it was shown that modification of one arginine residue was responsible for the inactivation. Almost 100% restoration of this ETF activity was achieved upon incubation with free arginine. However, the same 2,3-butanedione modified ETF only possessed decreased activity with dimethylglycine-(DMGDH, 44%) and sarcosine- (SDH, 27%) dehydrogenases unlike the abolition with MCAD. Full protection of ETF from arginine modification by 2,3-butanedione was achieved using substrate-protected DMGDH, MCAD and SDH respectively. Cross-protection studies of ETF with the three dehydrogenases implied use of the same single arginine residue in the binding of all three dehydrogenases. These results lead us to conclude that this single arginine residue is essential in the binding of the ETF to MCAD, but only contributes partially to the binding of ETF to SDH and DMGDH and thus, the determinants of the dehydrogenase binding sites overlap but are not identical.

78 FR 70976 - Horizons ETFs Management (USA) LLC and Horizons ETF Trust; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-27

... SECURITIES AND EXCHANGE COMMISSION [Investment Company Act Release No. 30803; 812-14179] Horizons ETFs Management (USA) LLC and Horizons ETF Trust; Notice of Application November 21, 2013. AGENCY: Securities and Exchange Commission (``Commission''). ACTION: Notice of an application for an order under...

Menstrual cycle worsening of epileptic seizures in women with symptomatic focal epilepsy.

PubMed

Bazán, Ana Carolina Belini; Montenegro, Maria Augusta; Cendes, Fernando; Min, Li Li; Guerreiro, Carlos A M

2005-09-01

Hormonal fluctuation is responsible for worsening of epileptic seizures during the menstrual cycle. To identify irregularities in the menstrual cycles of women with mesial temporal lobe epilepsy (MTLE) and extratemporal focal epilepsy (ETFE) and correlate the frequency of seizures during the menstrual cycles. We evaluated prospectively women in the menacme with MTLE and ETFE. Calendars were provided for these patients, and they were asked to mark their seizure frequency according to the menses. Calendars were reviewed in each routine medical appointment. Thirty-nine patients with MTLE and 14 with ETFE were evaluated. We registered 211 cycles in the patients with MTLE and 49 in those with ETFE. Irregular menstrual cycles were found in 28 (28/39, 71.7%) patients with MTLE and 6 (6/14, 42.8%) with ETFE (p=0.052). Premenstrual seizure worsening was observed in 46 (21.8%) patients with MTLE and 9 (18.3%) with ETFE (p=0.596). Menstrual worsening was observed in 47 (22.2%) patients with MTLE and 15 (30.6%) with ETFE (p=0.217). Ovulatory worsening was observed in 36 (17%) patients with MTLE and 13 (26.5%) with ETFE (p=0,126). Catamenial worsening was observed in 58 (27.4%) of the patients with MTLE and in 17 (34.7%) of the patients with ETFE (p=0.315). There was no difference between the group of patients with MTLE and ETFE regarding the frequency of irregular cycles and seizure worsening during the premenstrual, menstrual, catamenial or ovulatory periods.

Molecular study of electron transfer flavoprotein alpha-subunit deficiency in two Japanese children with different phenotypes of glutaric acidemia type II.

PubMed

Purevjav, E; Kimura, M; Takusa, Y; Ohura, T; Tsuchiya, M; Hara, N; Fukao, T; Yamaguchi, S

2002-09-01

Electron transfer flavoprotein is a mitochondrial matrix protein composed of alpha- and beta-subunits (ETF alpha and ETF beta, respectively). This protein transfers electrons between several mitochondrial dehydrogenases and the main respiratory chain via ETF dehydrogenase (ETF-DH). Defects in ETF or ETF-DH cause glutaric acidemias type II (GAII). We investigated the molecular basis of ETF alpha deficiency in two Japanese children with different clinical phenotypes using expression study. Patient 1 had the severe form of GAII, a compound heterozygote of two mutations: 799G to A (alpha G267R) and nonsense 7C to T (alpha R3X). Patient 2 had the mild form and carried two heterozygous mutations: 764G to T (alpha G255V) and 478delG (frameshift). Both patients had one each of missense mutations in one allele; the others were either nonsense or truncated. Restriction enzyme digestion assay using genomic DNAs from 100 healthy Japanese revealed that these mutations were all novel. No signal for ETF alpha was detected by immunoblotting in cases of missense mutants, while wild-type cDNA resulted in expression of ETF alpha protein. Transfection with wild-type ETF alpha cDNA into cultured cells from both patients elevated incorporation of radioisotope-labelled fatty acids. These four mutations were pathogenic for GAII and missense mutations, alpha G255V and alpha G267R were considered anecdotal for mild and severe forms, respectively.

Reactions of electron-transfer flavoprotein and electron-transfer flavoprotein: ubiquinone oxidoreductase.

PubMed Central

Ramsay, R R; Steenkamp, D J; Husain, M

1987-01-01

Electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF-Q oxidoreductase) catalyses the re-oxidation of reduced electron-transfer flavoprotein (ETF) with ubiquinone-1 (Q-1) as the electron acceptor. A kinetic assay for the enzyme was devised in which glutaryl-CoA in the presence of glutaryl-CoA dehydrogenase was used to reduce ETFox. and the reduction of Q-1 was monitored at 275 nm. The partial reactions involved in the overall assay system were examined. Glutaryl-CoA dehydrogenase catalyses the rapid reduction of ETFox. to the anionic semiquinone (ETF.-), but reduces ETF.- to the fully reduced form (ETFhq) at a rate that is about 6-fold lower. ETF.-, but not ETFhq, is directly re-oxidized by Q-1 at a rate that, depending on the steady-state concentration of ETF.-, may contribute significantly to the overall reaction. ETF-Q oxidoreductase catalyses rapid disproportionation of ETF.- with an equilibrium constant of about 1.0 at pH 7.8. In the presence of Q-1 it also catalyses the re-oxidation of ETFhq at a rate that is faster than that of the overall reaction. Rapid-scan experiments indicated the formation of ETF.-, but its fractional concentration in the early stages of the re-oxidation of ETFhq is low. The data indicate that the re-oxidation of ETFhq proceeds at a rate that is adequate to account for the overall rate of electron transfer from glutaryl-CoA to Q-1. An unusual property of ETF-Q oxidoreductase seems to be that it not only catalyses the re-oxidation of the reduced forms of ETF but also facilitates the complete reduction of ETFox. to ETFhq by disproportionation of the radical. PMID:3593226

Enteral tube feeding of patients with acute stroke: when does the risk of diarrhoea increase?

PubMed

Arevalo-Manso, J J; Martinez-Sanchez, P; Juarez-Martin, B; Fuentes, B; Ruiz-Ares, G; Sanz-Cuesta, B E; Parrilla-Novo, P; Diez-Tejedor, E

2014-12-01

We aimed to evaluate the relationship between the length of time acute stroke patients underwent enteral tube feeding (ETF) and episodes of diarrhoea, and to investigate the temporal cut-off point at which diarrhoea risk increases. An observational, retrospective study was conducted on patients with acute stroke admitted to a Stroke Centre. Patients undergoing ETF (ETF group) and those not undergoing ETF (control group) were analysed and matched by age and stroke severity. Data regarding demographic and clinical variables were recorded. The analysis was conducted using a receiver operating characteristic (ROC) curve and multivariate analyses. A total of 130 inpatients was included (age 75.08 ± 11.53 years, 56.2% men). The ETF group had higher diarrhoea frequency (27.7% vs 6.2%, P = 0.001). The length of time on ETF was associated with diarrhoea development (odds ratio (OR), 1.12 increment per day; 95% confidence interval (CI) 1.05-1.18; P < 0.001), after adjusting for confounders. The ROC curve showed 7 days on ETF as a cut-off point for diarrhoea risk. Seven days or more on ETF was independently associated with diarrhoea (OR, 6.26; 95% CI 1.66-23.62; P = 0.007), whereas less than 7 days was not when compared with the control group (OR, 0.38; 95% CI 0.04-3.91; P = 0.413). The length of time on ETF is associated with diarrhoea development in patients with acute stroke, demonstrating a temporal cut-off point. Seven days or longer on ETF is related to the occurrence of diarrhoea, whereas less than 7 days on ETF does not show this effect. © 2014 The Authors; Internal Medicine Journal © 2014 Royal Australasian College of Physicians.

Expression of human electron transfer flavoprotein-ubiquinone oxidoreductase from a baculovirus vector: kinetic and spectral characterization of the human protein.

PubMed

Simkovic, Martin; Degala, Gregory D; Eaton, Sandra S; Frerman, Frank E

2002-06-15

Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) is an iron-sulphur flavoprotein and a component of an electron-transfer system that links 10 different mitochondrial flavoprotein dehydrogenases to the mitochondrial bc1 complex via electron transfer flavoprotein (ETF) and ubiquinone. ETF-QO is an integral membrane protein, and the primary sequences of human and porcine ETF-QO were deduced from the sequences of the cloned cDNAs. We have expressed human ETF-QO in Sf9 insect cells using a baculovirus vector. The cDNA encoding the entire protein, including the mitochondrial targeting sequence, was present in the vector. We isolated a membrane-bound form of the enzyme that has a molecular mass identical with that of the mature porcine protein as determined by SDS/PAGE and has an N-terminal sequence that is identical with that predicted for the mature holoenzyme. These data suggest that the heterologously expressed ETF-QO is targeted to mitochondria and processed to the mature, catalytically active form. The detergent-solubilized protein was purified by ion-exchange and hydroxyapatite chromatography. Absorption and EPR spectroscopy and redox titrations are consistent with the presence of flavin and iron-sulphur centres that are very similar to those in the equivalent porcine and bovine proteins. Additionally, the redox potentials of the two prosthetic groups appear similar to those of the other eukaryotic ETF-QO proteins. The steady-state kinetic constants of human ETF-QO were determined with ubiquinone homologues, a ubiquinone analogue, and with human wild-type ETF and a Paracoccus-human chimaeric ETF as varied substrates. The results demonstrate that this expression system provides sufficient amounts of human ETF-QO to enable crystallization and mechanistic investigations of the iron-sulphur flavoprotein.

75 FR 1093 - Self-Regulatory Organizations; The Options Clearing Corporation; Notice of Filing of Proposed...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-08

... any option or any futures contracts on ETFS Physical Swiss Gold Shares and ETFS Physical Silver Shares... jurisdictional status of options or security futures on ETFS Physical Swiss Gold Shares or ETFS Physical Silver... approving a proposed rule change clarifying that options and securities futures on SPDR Gold Shares are...

77 FR 36599 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-19

... Change Relating to the Accuvest Global Long Short ETF (Formerly the Mars Hill Global Relative Value ETF...) applicable to, the Accuvest Global Long Short ETF (``Fund'') (formerly known as the Mars Hill Global Relative... the Exchange of shares (``Shares'') of the Mars Hill Global Relative Value ETF, a series of Advisor...

78 FR 54298 - Horizons ETFs Management (USA) LLC and Horizons ETF Trust; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-03

... SECURITIES AND EXCHANGE COMMISSION [Investment Company Act Release No. 30680; 812-14178] Horizons ETFs Management (USA) LLC and Horizons ETF Trust; Notice of Application August 27, 2013. AGENCY: Securities and Exchange Commission (``Commission''). ACTION: Notice of an application for an order under section 6(c) of the Investment Company Act of...

75 FR 39070 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-07

... index? If there are trading pauses in an ETF but not in the stocks that underlie that ETF, what... price discovery for the ETF, the underlying stocks and other products? Are there other market-based... Exchange Traded Funds (``ETF''),\\3\\ Exchange Traded Vehicles (``ETV''),\\4\\ and Exchange Traded Notes (``ETN...

75 FR 39084 - Self-Regulatory Organizations; EDGX Exchange, Inc.; Notice of Filing of Proposed Rule Change To...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-07

... there are trading pauses in an ETF but not in the stocks that underlie that ETF, what consequences might... discovery for the ETF, the underlying stocks and other products? Are there other market-based... filing, ETPs include Exchange Traded Funds (``ETF''),\\3\\ Exchange Traded Vehicles (``ETV''),\\4\\ and...

Surface modification of ethylene-co-tetrafluoroethylene copolymer (ETFE) by plasma

NASA Astrophysics Data System (ADS)

Inagaki, N.

2003-08-01

Surface modification of ETFE surfaces by remote H 2, O 2 and Ar plasmas were investigated from the viewpoint of selective modification of CH 2-CH 2 or CF 2-CF 2 component. The remote H 2 and Ar plasmas modified effectively ETFE surfaces into hydrophilic, but the remote O 2 plasma did not. The remote H 2 plasma interacted with CF 2 component rather than CH 2 component in ETFE. The remote O 2 plasma interacted with CH 2 component as well as CF 2 component in ETFE chains.

Contribution of ethylenetetrafluoroethylene (ETFE) insulation to the electrical performance of Riata® silicone leads having externalized conductors.

PubMed

Fischer, Avi; Klehn, Russell

2013-08-01

The insulation of St. Jude Medical Riata® leads contains a polytetrafluoroethylene (PTFE) liner, silicone tubing, and ethylenetetrafluoroethylene (ETFE) coating on individual cable conductors. ETFE has sufficient dielectric strength to assure electrical function. This investigation intended to analyze performance of leads with and without externalized conductors and with intact and breached ETFE. Testing was performed on ETFE-coated conductors to determine their ability to deliver high-voltage therapy. Tests were performed on samples under different conditions and current leakage was measured. A high-voltage test and a cyclic pulse test were performed, and the effect of lead modifications on the potential gradient from a high-voltage shock was used to determine functionality. Measurements from modified Riata® leads were compared with a control lead with all insulation and conducting elements intact. Current leakage for all conditions tested, was within the acceptance criteria for the high-voltage test and the cyclic pulse test. In conductors that underwent cyclic testing, the highest value of current leakage was within the limit of acceptability for both phases of the test. Testing of leads with externalized conductors and breached ETFE showed similar potential gradients compared with a control lead. Testing of ETFE-coated conductors following multiple preconditioning steps showed that ETFE serves as a redundant layer of insulation. In the event that the ETFE coating is breached, the potential gradient seen resulting from a high-voltage defibrillation shock was similar to a lead with no breach to the ETFE, even after 100 shocks.

Hydrogen-bonding dynamics of free flavins in benzene and FAD in electron-transferring flavoprotein upon excitation.

PubMed

Sato, Kyousuke; Nishina, Yasuzou; Shiga, Kiyoshi; Tanaka, Fumio

2003-01-01

The dynamic natures of two hydrogen-bonding model systems, riboflavin tetrabutylate (RFTB)-trichloroacetic acid (TCA) and RFTB-phenol in benzene, and of electron-transferring flavoprotein (ETF) from pig kidney upon excitation of flavins was investigated by means of steady state and time-resolved fluorescence spectroscopy. In both model systems fluorescence intensities of RFTB decreased as TCA or phenol was added. The spectral characteristics of ETF under steady state excitation were quite similar to those of the RFTB-TCA system, but not to those of the RFTB-phenol system. The observed fluorescence decay curves of ETF fit well with the calculated decay curves with two lifetime components, as in the model systems. Averaged lifetime was 0.9 ns. The time-resolved fluorescence spectrum of ETF shifted toward longer wavelength with time after pulsed excitation, which was also observed in the RFTB-TCA system. In the RFTB-phenol system the emission spectrum did not shift at all with time. These results reveal that the dynamic nature of ETF can be ascribed to aliphatic hydrogen-bonding(s) of the isoalloxazine ring with surrounding amino acid(s). From the fluorescence characteristics of ETF in comparison with the model systems, human ETF and other flavoproteins, it was suggested that ETF from pig kidney does not contain Tyr-16 in the beta subunit, unlike human ETF.

Parent, patient and health professional perspectives regarding enteral nutrition in paediatric oncology.

PubMed

Cohen, Jennifer; Wakefield, Claire E; Tapsell, Linda C; Walton, Karen; Cohn, Richard J

2017-11-01

Enteral tube feeding (ETF) is an important part of treatment for paediatric cancer patients. Without nutritional therapy, the prevalence of under-nutrition during treatment for childhood cancer may be as high as 50%. To ensure that the appropriate initiation of ETF is optimised, information on the views of key stakeholders regarding ETF is needed. In total, 48 interviews were conducted with parents of paediatric cancer patients (n = 20), patients (n = 10) and members of the paediatric oncology health-care team (n = 18). Semistructured interviews were used to elicit information from participants, and the data were analysed using a content analysis approach. The interviews focused on views regarding: (i) attitude toward, and impact of, ETF; (ii) information and support regarding ETF; and (iii) clinical management of ETF. There was agreement between stakeholders on the impact of ETF on patients, both positive (good nutrition, weight gain and decreased anxiety) and negative (physical appearance, invasive insertion procedure and comfort). There were discordant perceptions regarding the timing and type of information provided on the use of ETF, as well as the decision-making process used. By standardising the information given to parents and enhancing understanding of parent, patient and health-care worker perceptions about ETF, the initiation of tube feeding may be optimised. This may positively impact patient outcomes in the future. © 2017 Dietitians Association of Australia.

Extensive domain motion and electron transfer in the human electron transferring flavoprotein.medium chain Acyl-CoA dehydrogenase complex.

PubMed

Toogood, Helen S; van Thiel, Adam; Basran, Jaswir; Sutcliffe, Mike J; Scrutton, Nigel S; Leys, David

2004-07-30

The crystal structure of the human electron transferring flavoprotein (ETF).medium chain acyl-CoA dehydrogenase (MCAD) complex reveals a dual mode of protein-protein interaction, imparting both specificity and promiscuity in the interaction of ETF with a range of structurally distinct primary dehydrogenases. ETF partitions the functions of partner binding and electron transfer between (i) the recognition loop, which acts as a static anchor at the ETF.MCAD interface, and (ii) the highly mobile redox active FAD domain. Together, these enable the FAD domain of ETF to sample a range of conformations, some compatible with fast interprotein electron transfer. Disorders in amino acid or fatty acid catabolism can be attributed to mutations at the protein-protein interface. Crucially, complex formation triggers mobility of the FAD domain, an induced disorder that contrasts with general models of protein-protein interaction by induced fit mechanisms. The subsequent interfacial motion in the MCAD.ETF complex is the basis for the interaction of ETF with structurally diverse protein partners. Solution studies using ETF and MCAD with mutations at the protein-protein interface support this dynamic model and indicate ionic interactions between MCAD Glu(212) and ETF Arg alpha(249) are likely to transiently stabilize productive conformations of the FAD domain leading to enhanced electron transfer rates between both partners.

75 FR 39076 - Self-Regulatory Organizations; Notice of Filing of Proposed Rule Change by NYSE Arca, Inc...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-07

... index? If there are trading pauses in an ETF but not in the stocks that underlie that ETF, what... price discovery for the ETF, the underlying stocks and other products? Are there other market-based...'') to the pilot rule. For purposes of this filing, ETPs include Exchange Traded Funds (``ETF''),\\4...

Purification of electron-transferring flavoprotein from Megasphaera elsdenii and binding of additional FAD with an unusual absorption spectrum.

PubMed

Sato, Kyosuke; Nishina, Yasuzo; Shiga, Kiyoshi

2003-11-01

Electron-transferring flavoprotein (ETF), its redox partner flavoproteins, i.e., D-lactate dehydrogenase and butyryl-CoA dehydrogenase, and another well-known flavoprotein, flavodoxin, were purified from the same starting cell paste of an anaerobic bacterium, Megasphaera elsdenii. The purified ETF contained one mol FAD/mol ETF as the sole non-protein component and bound almost one mol of additional FAD. This preparation is a better subject for investigations of M. elsdenii ETF than the previously isolated ETF, which contains varying amounts of FAD and varying percentages of modified flavins such as 6-OH-FAD and 8-OH-FAD. The additionally bound FAD shows an anomalous absorption spectrum with strong absorption around 400 nm. This spectral change is not due to a chemical modification of the flavin ring because the flavin released by KBr or guanidine hydrochloride is normal FAD. It is also not due to unknown small molecules because the same spectrum appears when ETF is reconstituted from its guanidine-denatured subunits and FAD. A similar anomalous spectrum was observed for AMP-free pig ETF under acidic conditions, suggesting a common flavin environment between pig and M. elsdenii ETFs.

A construction of unimodular equiangular tight frames from resolvable Steiner systems

NASA Astrophysics Data System (ADS)

Jasper, John

2013-09-01

An equiangular tight frame (ETF) is an M x N matrix which has orthogonal equal norm rows, equal norm columns, and the inner products of all pairs of columns have the same modulus. In this paper we study ETFs in which all of the entries are unimodular, and in particular pth roots of unity. A new construction of unimodular ETFs based on resolvable Steiner systems is presented. This construction gives many new examples of unimodular ETFs. In particular, an new infinite class of ETFs with entries in f1;-1g is presented.

78 FR 62834 - Self-Regulatory Organizations; The NASDAQ Stock Market LLC; Order Granting Approval of Proposed...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-22

... in inverse ETFs, the Fund will not invest in leveraged or inverse leveraged (e.g., 2X or -3X) ETFs..., according to the Exchange, the Fund's ownership and control of the First Trust Subsidiary will prevent the.... The Fund may invest in inverse ETFs, but it will not invest in leveraged or inverse leveraged ETFs...

ETF Yearbook 2012: Evaluation and Monitoring of Vocational Education and Training Systems and the Role of Evidence-Based Policy in Their Reforms

ERIC Educational Resources Information Center

European Training Foundation, 2012

2012-01-01

The "ETF Yearbook 2012" continues the tradition of highlighting a thematic field of particular importance to the work of the European Training Foundation (ETF). The theme of this yearbook is evaluation and monitoring of vocational education and training (VET) systems and the role of evidence-based policy in VET reforms in ETF partner…

77 FR 73500 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing of Proposed Rule Change Relating...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-10

... Trading of Shares of the Horizons S&P 500 Covered Call ETF, Horizons S&P Financial Select Sector Covered Call ETF, and Horizons S&P Energy Select Sector Covered Call ETF Under NYSE Arca Equities Rule 5.2(j)(3... and trade shares (``Shares'') of the Horizons S&P 500 Covered Call ETF, Horizons S&P Financial Select...

Electron transfer and conformational change in complexes of trimethylamine dehydrogenase and electron transferring flavoprotein.

PubMed

Jones, Matthew; Talfournier, Francois; Bobrov, Anton; Grossmann, J Günter; Vekshin, Nikolai; Sutcliffe, Michael J; Scrutton, Nigel S

2002-03-08

The trimethylamine dehydrogenase-electron transferring flavoprotein (TMADH.ETF) electron transfer complex has been studied by fluorescence and absorption spectroscopies. These studies indicate that a series of conformational changes occur during the assembly of the TMADH.ETF electron transfer complex and that the kinetics of assembly observed with mutant TMADH (Y442F/L/G) or ETF (alpha R237A) complexes are much slower than are the corresponding rates of electron transfer in these complexes. This suggests that electron transfer does not occur in the thermodynamically most favorable state (which takes too long to form), but that one or more metastable states (which are formed more rapidly) are competent in transferring electrons from TMADH to ETF. Additionally, fluorescence spectroscopy studies of the TMADH.ETF complex indicate that ETF undergoes a stable conformational change (termed structural imprinting) when it interacts transiently with TMADH to form a second, distinct, structural form. The mutant complexes compromise imprinting of ETF, indicating a dependence on the native interactions present in the wild-type complex. The imprinted form of semiquinone ETF exhibits an enhanced rate of electron transfer to the artificial electron acceptor, ferricenium. Overall molecular conformations as probed by small-angle x-ray scattering studies are indistinguishable for imprinted and non-imprinted ETF, suggesting that changes in structure likely involve confined reorganizations within the vicinity of the FAD. Our results indicate a series of conformational events occur during the assembly of the TMADH.ETF electron transfer complex, and that the properties of electron transfer proteins can be affected lastingly by transient interaction with their physiological redox partners. This may have significant implications for our understanding of biological electron transfer reactions in vivo, because ETF encounters TMADH at all times in the cell. Our studies suggest that caution needs to be exercised in extrapolating the properties of in vitro interprotein electron transfer reactions to those occurring in vivo.

Sensitivity and specificity of eustachian tube function tests in adults.

PubMed

Doyle, William J; Swarts, J Douglas; Banks, Julianne; Casselbrant, Margaretha L; Mandel, Ellen M; Alper, Cuneyt M

2013-07-01

The study demonstrates the utility of eustachian tube (ET) function (ETF) test results for accurately assigning ears to disease state. To determine if ETF tests can identify ears with physician-diagnosed ET dysfunction (ETD) in a mixed population at high sensitivity and specificity and to define the interrelatedness of ETF test parameters. Through use of the forced-response, inflation-deflation, Valsalva, and sniffing tests, ETF was evaluated in 15 control ears of adult subjects after unilateral myringotomy (group 1) and in 23 ears of 19 adult subjects with ventilation tubes inserted for ETD (group 2). Data were analyzed using logistic regression including each parameter independently and then a step-down discriminant analysis including all ETF test parameters to predict group assignment. Factor analysis operating over all parameters was used to explore relatedness. ETF testing. ETF parameters for the forced response, inflation-deflation, Valsalva, and sniffing tests measured in 15 control ears of adult subjects after unilateral myringotomy (group 1) and in 23 ears of 19 adult subjects with ventilation tubes inserted for ETD (group 2). The discriminant analysis identified 4 ETF test parameters (Valsalva, ET opening pressure, dilatory efficiency, and percentage of positive pressure equilibrated) that together correctly assigned ears to group 2 at a sensitivity of 95% and a specificity of 83%. Individual parameters representing the efficiency of ET opening during swallowing showed moderately accurate assignments of ears to their respective groups. Three factors captured approximately 98% of the variance among parameters: the first had negative loadings of the ETF structural parameters; the second had positive loadings of the muscle-assisted ET opening parameters; and the third had negative loadings of the muscle-assisted ET opening parameters and positive loadings of the structural parameters. These results show that ETF tests can correctly assign individual ears to physician-diagnosed ETD with high sensitivity and specificity and that ETF test parameters can be grouped into structural-functional categories.

Dynamical theory of responsivity and response time of a high temperature superconductor photo-thermoelectrical bolometer

NASA Astrophysics Data System (ADS)

Kaila, M. M.

2002-11-01

Dynamical theory of responsivity and response time for an high temperature superconductor (HTSC) photo-thermoelectrical bolometer is analysed in this paper. There is a thermoelectric feedback (TEF) due to the heat transfer from the sensitive area (HTSC-BiSb thermojunction) towards the cold junction of the thermocouple. This is in addition to the normal electrothermal feedback (ETF) between the detector and the substrate, in a photoelectrical bolometer. The two legs of the thermocouple are connected in a parallel geometry configuration. It is seen that TEF can be used in combination with the ETF to enhance responsivity and response time of the detector.

Weld Repair of a Stamped Pressure Vessel in a Radiologically Controlled Zone

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cannell, Gary L.; Huth, Ralph J.; Hallum, Randall T.

2013-08-26

In September 2012 an ASME B&PVC Section VIII stamped pressure vessel located at the DOE Hanford Site Effluent Treatment Facility (ETF) developed a through-wall leak. The vessel, a steam/brine heat exchanger, operated in a radiologically controlled zone (by the CH2MHill PRC or CHPRC), had been in service for approximately 17 years. The heat exchanger is part of a single train evaporator process and its failure caused the entire system to be shut down, significantly impacting facility operations. This paper describes the activities associated with failure characterization, technical decision making/planning for repair by welding, logistical challenges associated with performing work inmore » a radiologically controlled zone, performing the repair, and administrative considerations related to ASME code requirements.« less

Radiolytic preparation of ETFE and PFA based anion exchange membranes for alkaline fuel cell

NASA Astrophysics Data System (ADS)

Ko, Beom-Seok; Sohn, Joon-Yong; Nho, Young-Chang; Shin, Junhwa

2011-11-01

In this study, a versatile monomer, vinylbenzyl chloride (VBC) was radiolytically grafted onto a partially fluorinated ETFE and perfluorinated polymer PFA films. The VBC grafted films were treated with trimethylamine to prepare the alkaline anion exchange membranes (AAEMs). No significant differences in the ion exchange capacities and water uptakes were observed between the ETFE and PFA based AAEMs with similar degree of grafting (DOG). However, the distribution patterns of the graft chains over the cross-section of the ETFE and PFA based AAEMs were found to be quite different; the even distribution was observed from the ETFE based AAEMs while the uneven distribution was observed from the PFA based AAEMs. It was also found that the PFA based AAEMs have the higher ionic conductivity and chemical stability, compared to the ETFE based AAEMs.

Centralized PI control for high dimensional multivariable systems based on equivalent transfer function.

PubMed

Luan, Xiaoli; Chen, Qiang; Liu, Fei

2014-09-01

This article presents a new scheme to design full matrix controller for high dimensional multivariable processes based on equivalent transfer function (ETF). Differing from existing ETF method, the proposed ETF is derived directly by exploiting the relationship between the equivalent closed-loop transfer function and the inverse of open-loop transfer function. Based on the obtained ETF, the full matrix controller is designed utilizing the existing PI tuning rules. The new proposed ETF model can more accurately represent the original processes. Furthermore, the full matrix centralized controller design method proposed in this paper is applicable to high dimensional multivariable systems with satisfactory performance. Comparison with other multivariable controllers shows that the designed ETF based controller is superior with respect to design-complexity and obtained performance. Copyright © 2014 ISA. Published by Elsevier Ltd. All rights reserved.

Embryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the beta1 subunit of sodium-potassium ATPase in mouse embryos.

PubMed

Xu, J S; Lee, Y L; Lee, K F; Kwok, K L; Lee, W M; Luk, J M; Yeung, W S B

2004-12-01

Embrytrophic factor-3 (ETF-3) from human oviductal cells enhanced the development of mouse preimplantation embryos. This report studied the embryotrophic mechanisms of the molecule. Mouse embryos were incubated with ETF-3 for 24 h at different stages of development. ETF-3 treatment between 96 and 120 h post-HCG increased the cell count of blastocysts, whilst treatment between 72 and 96 h post-HCG enhanced the expansion and hatching of the blastocysts. ETF-3 increased the cell number of the embryos by suppressing apoptosis and increasing proliferation as determined by TUNEL and bromodeoxyuridine uptake assays, respectively. Real-time quantitative PCR showed that the in vivo developed and ETF-3-treated blastocysts had a significantly higher mRNA copy number of Na/K-ATPase-beta1, but not of hepsin, than that of blastocysts cultured in medium alone. The former gene was associated with cavitation of blastocysts while the latter was related to hatching of blastocyst. The beneficial effect of ETF-3 on blastocyst hatching was also seen when ETF-3-supplemented commercially available sequential culture medium for human embryo culture was used to culture mouse embryos. ETF-3 improves embryo development by enhancing proliferation, suppressing apoptosis and stimulating expression of genes related to blastocyst cavitation. Supplementating human embryo culture medium with ETF-3 may improve the success rate in clinical assisted reproduction.

Proactive enteral tube feeding in pediatric patients undergoing chemotherapy.

PubMed

Sacks, Nancy; Hwang, Wei-Ting; Lange, Beverly J; Tan, Kay-See; Sandler, Eric S; Rogers, Paul C; Womer, Richard B; Pietsch, John B; Rheingold, Susan R

2014-02-01

To determine feasibility and safety of proactive enteral tube feeding (ETF) in pediatric oncology patients. Pediatric patients with newly diagnosed brain tumors, myeloid leukemia or high-risk solid tumors were eligible. Subjects agreeing to start ETF before cycle 2 chemotherapy were considered proactive participants (PPs). Those who declined could enroll as chart collection receiving nutritional standard of care. Nutritional status was assessed using standard anthropometric measurements. Episodes of infection and toxicity related to ETF were documented from diagnosis to end of therapy. A descriptive comparison between PPs and controls was conducted. One hundred four eligible patients were identified; 69 enrolled (20 PPs and 49 controls). At diagnosis, 17% of all subjects were underweight and 26% overweight. Barriers to enrollment included physician, subject and/or family refusal, and inability to initiate ETF prior to cycle 2 of chemotherapy. Toxicity of ETF was minimal, but higher percentage of subjects in the proactive group had episodes of infection than controls. Thirty-nine percent of controls eventually started ETF and were twice as likely to receive parenteral nutrition. PPs experienced less weight loss at ETF initiation than controls receiving ETF and were the only group to demonstrate improved nutritional status at end of study. Proactive ETF is feasible in children with cancer and results in improved nutritional status at end of therapy. Episodes of infection in this study are concerning; therefore, a larger randomized trial is required to further delineate infectious risks and toxicities that may be mitigated by improved nutritional status. © 2013 Wiley Periodicals, Inc.

The interaction of trimethylamine dehydrogenase and electron-transferring flavoprotein.

PubMed

Shi, Weiwei; Mersfelder, John; Hille, Russ

2005-05-27

The interaction between the physiological electron transfer partners trimethylamine dehydrogenase (TMADH) and electron-transferring flavoprotein (ETF) from Methylophilus methylotrophus has been examined with particular regard to the proposal that the former protein "imprints" a conformational change on the latter. The results indicate that the absorbance change previously attributed to changes in the environment of the FAD of ETF upon binding to TMADH is instead caused by electron transfer from partially reduced, as-isolated TMADH to ETF. Prior treatment of the as-isolated enzyme with the oxidant ferricenium essentially abolishes the observed spectral change. Further, when the semiquinone form of ETF is used instead of the oxidized form, the mirror image of the spectral change seen with as-isolated TMADH and oxidized ETF is observed. This is attributable to a small amount of electron transfer in the reverse of the physiological direction. Kinetic determination of the dissociation constant and limiting rate constant for electron transfer within the complex of (reduced) TMADH with (oxidized) ETF is reconfirmed and discussed in the context of a recently proposed model for the interaction between the two proteins that involves "structural imprinting" of ETF.

78 FR 19542 - Neuberger Berman ETF Trust and Neuberger Berman Management LLC; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-01

...] Neuberger Berman ETF Trust and Neuberger Berman Management LLC; Notice of Application March 25, 2013. AGENCY... ETF Trust (the ``Trust'') and Neuberger Berman Management LLC (``NBM'' or [[Page 19543

Assignment of electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) to human chromosome 4q33 by fluorescence in situ hybridization and somatic cell hybridization.

PubMed

Spector, E B; Seltzer, W K; Goodman, S I

1999-08-01

Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) is a nuclear-encoded protein located in the inner mitochondrial membrane. Inherited defects of ETF-QO cause glutaric acidemia type II. We here describe the localization of the ETF-QO gene to human chromosome 4q33 by somatic cell hybridization and fluorescence in situ hybridization. Copyright 1999 Academic Press.

[Effect of human oviductal embryotrophic factors on gene expression of mouse preimplantation embryos].

PubMed

Yao, Yuan-Qing; Lee, Kai-Fai; Xu, Jia-Seng; Ho, Pak-Chung; Yeung, Shu-Biu

2007-09-01

To investigate the effect of embryotrophic factors (ETF) from human oviductal cells on gene expression of mouse early developmental embryos and discuss the role of fallopian tube in early development of embryos. ETF was isolated from conditioned medium of human oviductal cell line by sequential liquid chromatographic systems. Mouse embryos were treated by ETF in vitro. Using differential display RT-PCR, the gene expression of embryos treated by ETF was compared with embryos without ETF treatment. The differentially expressed genes were separated, re-amplified, cloned and sequenced. Gene expression profiles of embryos with ETF treatment was different from embryos without this treatment. Eight differentially expressed genes were cloned and sequenced. These genes functioned in RNA degradation, synthesis, splicing, protein trafficking, cellular differentiation and embryo development. Embryotrophic factors from human oviductal cells affect gene expression of early developmental embryos. The human oviductal cells play wide roles in early developmental stages of embryos.

Isomers in the excited state of electron-transferring flavoprotein from Megasphaera elsdenii: spectral resolution from the time-resolved fluorescence spectra.

PubMed

Sato, Kyosuke; Nishina, Yasuzo; Shiga, Kiyoshi; Tanaka, Fumio

2008-02-27

Electron-transferring flavoprotein (Holo-ETF) from Megasphaera elsdenii contains two FAD's, one of which easily dissociates to form Iso-ETF (contains one FAD). Time-resolved fluorescence of FAD in Iso-ETF, and Holo-ETF were measured at 5 degrees C and 25 degrees C. Wavelength-dependent fluorescence decays of the both ETF at 5 degrees C and 25 degrees C were analyzed to resolve them into two independent spectra. It was found that Iso-ETF displayed two spectra with lifetime of 0.605 ns (emission peak, 508 nm) and with lifetime of 1.70 ns (emission peak, 540 nm) at 5 degrees C, and with lifetime of 0.693 ns (emission peak, 508 nm) and with lifetime of 2.75 ns (emission peak, 540 nm) at 25 degrees C. Holo-ETF displayed two spectra with lifetime of 0.739 ns (emission peak, 508 nm) and with lifetime of 2.06 ns (emission peak, 545 nm) at 5 degrees C, and with lifetime of 0.711 ns (emission peak, 527 nm) and with lifetime of 3.08 ns (emission peak, 540 nm) at 25 degrees C. Thus fluorescence lifetimes of every spectrum increased upon elevating temperature. Emission peaks Iso-ETF did not change much upon elevating temperature. Activation enthalpy changes, activation entropy changes and activation Gibbs energy changes of quenching rates all displayed negative. Two emission species in the both ETF may be hydrogen-bonding isomers, because isoalloxazine ring of FAD contains four hydrogen acceptors and one donor.

Factors associated with nosocomial diarrhea in patients with enteral tube feeding.

PubMed

Trabal, J; Leyes, P; Hervás, S; Herrera, M; de Talló Forga, Ma

2008-01-01

Diarrhea is a frequent complication associated to enteral tube feeding (ETF) and it is a frequent cause of reduction or suspension of this type of nutritional support. Our objective was to evaluate the factors associated with nosocomial diarrhea in patients receiving ETF. The only significant factor associated with the appearance of diarrhea was antibiotic consumption, specially those patients receiving the combination of two or more antibiotics. We did not find any association between factors related to ETF, analytical parameters, nor other medications and diarrhea. Our observational data supports the idea that ETF should not be seen as a primary cause of diarrhea, other possible causes should be considered before reducing or discontinuing ETF administration.

Quasi-symmetric designs and equiangular tight frames

NASA Astrophysics Data System (ADS)

Fickus, Matthew; Jasper, John; Mixon, Dustin; Peterson, Jesse

2015-08-01

An equiangular tight frame (ETF) is an M×N matrix which has orthogonal equal norm rows, equal norm columns, and the inner products of all pairs of columns have the same modulus. ETFs arise in numerous applications, including compressed sensing. They also seem to be rare: despite over a decade of active research by the community, only a few construction methods have been discovered. In this article we introduce a new construction of ETFs which uses a particular set of combinatorial designs called quasi-symmetric designs. For ETFs whose entries are contained in {+1;-1}, called real constant amplitude ETFs (RCAETFs), we see that this construction is reversible, giving new quasi-symmetric designs from the known constructions RCAETFs.

Interaction between NADH and electron-transferring flavoprotein from Megasphaera elsdenii.

PubMed

Sato, Kyosuke; Nishina, Yasuzo; Shiga, Kiyoshi

2013-06-01

Electron-transferring flavoprotein (ETF) from the anaerobic bacterium Megasphaera elsdenii is a heterodimer containing two FAD cofactors. Isolated ETF contains only one FAD molecule, FAD-1, because the other, FAD-2, is lost during purification. FAD-2 is recovered by adding FAD to the isolated ETF. The two FAD molecules in holoETF were characterized using NADH. Spectrophotometric titration of isolated ETF with NADH showed a two-electron reduction of FAD-1 according to a monophasic profile indicating that FAD-1 receives electrons from NADH without involvement of FAD-2. When holoETF was titrated with NADH, FAD-2 was reduced to an anionic semiquinone and then was fully reduced before the reduction of FAD-1. The midpoint potential values at pH 7 were +81, -136 and -279 mV for the reduction of oxidized FAD-2 to semiquinone, semiquinone to the fully reduced FAD-2 and the two-electron reduction of FAD-1, respectively. Both FAD-1 and FAD-2 in holoETF were reduced by excess NADH very rapidly. The reduction of FAD-2 was slowed by replacement of FAD-1 with 8-cyano-FAD indicating that FAD-2 receives electrons from FAD-1 but not from NADH directly. The present results suggest that FAD-2 is the counterpart of the FAD in human ETF, which contains one FAD and one AMP.

Synthesis efficiency of heavy carbon clusters from ETFE ablated by different numbers of laser pulse in vacuum

NASA Astrophysics Data System (ADS)

Shibagaki, K.; Takada, N.; Sasaki, K.; Kadota, K.

2002-09-01

We have carried out mass spectral analysis of positive ions produced by laser ablation of a copolymer of ethylene and tetrafluoroethylene (ETFE: [CH 2CH 2CF 2CF 2] n) in vacuum using time-of-flight mass spectrometry (TOF-MS). The surfaces of the ETFE targets irradiated by different numbers of laser pulse were analyzed by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Heavy carbon cluster ions C n+ with n≥30 were observed in the mass spectra. The fractional abundance of heavy clusters in the mass spectrum decreased with the number of laser pulse. On the other hand, carbon became rich in the atomic composition of the laser-irradiated surface, and the eroded area on the surface increased with the number of laser pulse. From these results, it is suggested that the carbon-rich material surface results in the less efficient production of heavy carbon clusters. In addition, it is also suggested that clustering reactions in eroded craters do not contribute to the synthesis of heavy clusters.

Structure-property correlations of ion-containing polymers for fuel cell applications

NASA Astrophysics Data System (ADS)

Sproll, Véronique; Nagy, Gergely; Gasser, Urs; Balog, Sandor; Gustavsson, Sanna; Schmidt, Thomas J.; Gubler, Lorenz

2016-01-01

In order to investigate the structure-property correlations of grafted proton conducting membranes, the model system consisting of an ETFE base film grafted with polystyrene and subsequent sulfonation (ETFE-g-PSSA) along with crosslinked derivatives ETFE-g-P(SSA-co-DiPB) were synthesized. The characteristics of the final membranes were characterized by PFG-NMR diffusometry, in-plane conductivity and by investigations of the dimensional changes of the grafted membranes. The collected data were correlated with the inherent anisotropy of the ETFE base film.

Alternative quinone substrates and inhibitors of human electron-transfer flavoprotein-ubiquinone oxidoreductase.

PubMed Central

Simkovic, Martin; Frerman, Frank E

2004-01-01

Electron-transfer flavoprotein (ETF)-ubiquinone (2,3-dimethoxy-5-methyl-1,4-benzoquinone) oxidoreductase (ETF-QO) is a membrane-bound iron-sulphur flavoprotein that participates in an electron-transport pathway between eleven mitochondrial flavoprotein dehydrogenases and the ubiquinone pool. ETF is the intermediate electron carrier between the dehydrogenases and ETF-QO. The steady-state kinetic constants of human ETF-QO were determined with ubiquinone homologues and analogues that contained saturated n-alkyl substituents at the 6 position. These experiments show that optimal substrates contain a ten-carbon-atom side chain, consistent with a preliminary crystal structure that shows that only the first two of ten isoprene units of co-enzyme Q10 (CoQ10) interact with the protein. Derivatives with saturated alkyl side chains are very good substrates, indicating that, unlike other ubiquinone oxidoreductases, there is little preference for the methyl branches or rigidity of the CoQ side chain. Few of the compounds that inhibit ubiquinone oxidoreductases inhibit ETF-QO. Compounds found to act as inhibitors of ETF-QO include 2-n-heptyl-4-hydroxyquinoline N-oxide, a naphthoquinone analogue, 2-(3-methylpentyl)-4,6-dinitrophenol and pentachlorophenol. 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), which inhibits the mitochondrial bc1 complex and the chloroplast b6 f complex in redox-dependent fashion, can serve as an electron acceptor for human ETF-QO. The observation of simple Michaelis-Menten kinetic patterns and a single type of quinone-binding site, determined by fluorescence titrations of the protein with DBMIB and 6-(10-bromodecyl)ubiquinone, are consistent with one ubiquinone-binding site per ETF-QO monomer. PMID:14640977

alpha Arg-237 in Methylophilus methylotrophus (sp. W3A1) electron-transferring flavoprotein affords approximately 200-millivolt stabilization of the FAD anionic semiquinone and a kinetic block on full reduction to the dihydroquinone.

PubMed

Talfournier, F; Munro, A W; Basran, J; Sutcliffe, M J; Daff, S; Chapman, S K; Scrutton, N S

2001-06-08

The midpoint reduction potentials of the FAD cofactor in wild-type Methylophilus methylotrophus (sp. W3A1) electron-transferring flavoprotein (ETF) and the alphaR237A mutant were determined by anaerobic redox titration. The FAD reduction potential of the oxidized-semiquinone couple in wild-type ETF (E'(1)) is +153 +/- 2 mV, indicating exceptional stabilization of the flavin anionic semiquinone species. Conversion to the dihydroquinone is incomplete (E'(2) < -250 mV), because of the presence of both kinetic and thermodynamic blocks on full reduction of the FAD. A structural model of ETF (Chohan, K. K., Scrutton, N. S., and Sutcliffe, M. J. (1998) Protein Pept. Lett. 5, 231-236) suggests that the guanidinium group of Arg-237, which is located over the si face of the flavin isoalloxazine ring, plays a key role in the exceptional stabilization of the anionic semiquinone in wild-type ETF. The major effect of exchanging alphaArg-237 for Ala in M. methylotrophus ETF is to engineer a remarkable approximately 200-mV destabilization of the flavin anionic semiquinone (E'(2) = -31 +/- 2 mV, and E'(1) = -43 +/- 2 mV). In addition, reduction to the FAD dihydroquinone in alphaR237A ETF is relatively facile, indicating that the kinetic block seen in wild-type ETF is substantially removed in the alphaR237A ETF. Thus, kinetic (as well as thermodynamic) considerations are important in populating the redox forms of the protein-bound flavin. Additionally, we show that electron transfer from trimethylamine dehydrogenase to alphaR237A ETF is severely compromised, because of impaired assembly of the electron transfer complex.

Alternative quinone substrates and inhibitors of human electron-transfer flavoprotein-ubiquinone oxidoreductase.

PubMed

Simkovic, Martin; Frerman, Frank E

2004-03-01

Electron-transfer flavoprotein (ETF)-ubiquinone (2,3-dimethoxy-5-methyl-1,4-benzoquinone) oxidoreductase (ETF-QO) is a membrane-bound iron-sulphur flavoprotein that participates in an electron-transport pathway between eleven mitochondrial flavoprotein dehydrogenases and the ubiquinone pool. ETF is the intermediate electron carrier between the dehydrogenases and ETF-QO. The steady-state kinetic constants of human ETF-QO were determined with ubiquinone homologues and analogues that contained saturated n-alkyl substituents at the 6 position. These experiments show that optimal substrates contain a ten-carbon-atom side chain, consistent with a preliminary crystal structure that shows that only the first two of ten isoprene units of co-enzyme Q10 (CoQ10) interact with the protein. Derivatives with saturated alkyl side chains are very good substrates, indicating that, unlike other ubiquinone oxidoreductases, there is little preference for the methyl branches or rigidity of the CoQ side chain. Few of the compounds that inhibit ubiquinone oxidoreductases inhibit ETF-QO. Compounds found to act as inhibitors of ETF-QO include 2-n-heptyl-4-hydroxyquinoline N-oxide, a naphthoquinone analogue, 2-(3-methylpentyl)-4,6-dinitrophenol and pentachlorophenol. 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), which inhibits the mitochondrial bc1 complex and the chloroplast b6 f complex in redox-dependent fashion, can serve as an electron acceptor for human ETF-QO. The observation of simple Michaelis-Menten kinetic patterns and a single type of quinone-binding site, determined by fluorescence titrations of the protein with DBMIB and 6-(10-bromodecyl)ubiquinone, are consistent with one ubiquinone-binding site per ETF-QO monomer.

Structure of electron transfer flavoprotein-ubiquinone oxidoreductase and electron transfer to the mitochondrial ubiquinone pool

PubMed Central

Zhang, Jian; Frerman, Frank E.; Kim, Jung-Ja P.

2006-01-01

Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) is a 4Fe4S flavoprotein located in the inner mitochondrial membrane. It catalyzes ubiquinone (UQ) reduction by ETF, linking oxidation of fatty acids and some amino acids to the mitochondrial respiratory chain. Deficiencies in ETF or ETF-QO result in multiple acyl-CoA dehydrogenase deficiency, a human metabolic disease. Crystal structures of ETF-QO with and without bound UQ were determined, and they are essentially identical. The molecule forms a single structural domain. Three functional regions bind FAD, the 4Fe4S cluster, and UQ and are closely packed and share structural elements, resulting in no discrete structural domains. The UQ-binding pocket consists mainly of hydrophobic residues, and UQ binding differs from that of other UQ-binding proteins. ETF-QO is a monotopic integral membrane protein. The putative membrane-binding surface contains an α-helix and a β-hairpin, forming a hydrophobic plateau. The UQ—flavin distance (8.5 Å) is shorter than the UQ—cluster distance (18.8 Å), and the very similar redox potentials of FAD and the cluster strongly suggest that the flavin, not the cluster, transfers electrons to UQ. Two possible electron transfer paths can be envisioned. First, electrons from the ETF flavin semiquinone may enter the ETF-QO flavin one by one, followed by rapid equilibration with the cluster. Alternatively, electrons may enter via the cluster, followed by equilibration between centers. In both cases, when ETF-QO is reduced to a two-electron reduced state (one electron at each redox center), the enzyme is primed to reduce UQ to ubiquinol via FAD. PMID:17050691

Structure of electron transfer flavoprotein-ubiquinone oxidoreductase and electron transfer to the mitochondrial ubiquinone pool.

PubMed

Zhang, Jian; Frerman, Frank E; Kim, Jung-Ja P

2006-10-31

Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) is a 4Fe4S flavoprotein located in the inner mitochondrial membrane. It catalyzes ubiquinone (UQ) reduction by ETF, linking oxidation of fatty acids and some amino acids to the mitochondrial respiratory chain. Deficiencies in ETF or ETF-QO result in multiple acyl-CoA dehydrogenase deficiency, a human metabolic disease. Crystal structures of ETF-QO with and without bound UQ were determined, and they are essentially identical. The molecule forms a single structural domain. Three functional regions bind FAD, the 4Fe4S cluster, and UQ and are closely packed and share structural elements, resulting in no discrete structural domains. The UQ-binding pocket consists mainly of hydrophobic residues, and UQ binding differs from that of other UQ-binding proteins. ETF-QO is a monotopic integral membrane protein. The putative membrane-binding surface contains an alpha-helix and a beta-hairpin, forming a hydrophobic plateau. The UQ-flavin distance (8.5 A) is shorter than the UQ-cluster distance (18.8 A), and the very similar redox potentials of FAD and the cluster strongly suggest that the flavin, not the cluster, transfers electrons to UQ. Two possible electron transfer paths can be envisioned. First, electrons from the ETF flavin semiquinone may enter the ETF-QO flavin one by one, followed by rapid equilibration with the cluster. Alternatively, electrons may enter via the cluster, followed by equilibration between centers. In both cases, when ETF-QO is reduced to a two-electron reduced state (one electron at each redox center), the enzyme is primed to reduce UQ to ubiquinol via FAD.

Electron transfer flavoprotein domain II orientation monitored using double electron-electron resonance between an enzymatically reduced, native FAD cofactor, and spin labels

PubMed Central

Swanson, Michael A; Kathirvelu, Velavan; Majtan, Tomas; Frerman, Frank E; Eaton, Gareth R; Eaton, Sandra S

2011-01-01

Human electron transfer flavoprotein (ETF) is a soluble mitochondrial heterodimeric flavoprotein that links fatty acid β-oxidation to the main respiratory chain. The crystal structure of human ETF bound to medium chain acyl-CoA dehydrogenase indicates that the flavin adenine dinucleotide (FAD) domain (αII) is mobile, which permits more rapid electron transfer with donors and acceptors by providing closer access to the flavin and allows ETF to accept electrons from at least 10 different flavoprotein dehydrogenases. Sequence homology is high and low-angle X-ray scattering is identical for Paracoccus denitrificans (P. denitrificans) and human ETF. To characterize the orientations of the αII domain of P. denitrificans ETF, distances between enzymatically reduced FAD and spin labels in the three structural domains were measured by double electron-electron resonance (DEER) at X- and Q-bands. An FAD to spin label distance of 2.8 ± 0.15 nm for the label in the FAD-containing αII domain (A210C) agreed with estimates from the crystal structure (3.0 nm), molecular dynamics simulations (2.7 nm), and rotamer library analysis (2.8 nm). Distances between the reduced FAD and labels in αI (A43C) were between 4.0 and 4.5 ± 0.35 nm and for βIII (A111C) the distance was 4.3 ± 0.15 nm. These values were intermediate between estimates from the crystal structure of P. denitrificans ETF and a homology model based on substrate-bound human ETF. These distances suggest that the αII domain adopts orientations in solution that are intermediate between those which are observed in the crystal structures of free ETF (closed) and ETF bound to a dehydrogenase (open). PMID:21308847

Quantitative imaging of electron transfer flavoprotein autofluorescence reveals the dynamics of lipid partitioning in living pancreatic islets.

PubMed

Lam, Alan K; Silva, Pamuditha N; Altamentova, Svetlana M; Rocheleau, Jonathan V

2012-08-01

Pancreatic islet β-cells metabolically sense nutrients to maintain blood glucose homeostasis through the regulated secretion of insulin. Long-term exposure to a mixed supply of excess glucose and fatty acids induces β-cell dysfunction and type II diabetes in a process termed glucolipotoxicity. Despite a number of documented mechanisms for glucolipotoxicity, the interplay between glucose and fatty acid oxidation in islets remains debated. Here, we develop confocal imaging of electron transfer flavoprotein (ETF) autofluorescence to reveal the dynamics of fatty acid oxidation in living pancreatic islets. This method further integrates microfluidic devices to hold the islets stationary in flow, and thus achieve ETF imaging in the β-cells with high spatial and temporal resolution. Our data first confirm that ETF autofluorescence reflects electron transport chain (ETC) activity downstream of Complex I, consistent with a response directly related to fatty acid metabolism. Together with two-photon imaging of NAD(P)H and confocal imaging of lipoamide dehydrogenase (LipDH) autofluorescence, we show that the ETC predominantly draws electrons from LipDH/NADH-dependent Complex I rather than from ETF/FADH(2)-dependent ETF:CoQ oxidoreductase (ETF-QO). Islets stimulated with palmitate also show increased ETF redox state that is dose-dependently diminished by glucose (>10 mM). Furthermore, stimulation with a glucose bolus causes a two-tier drop in the ETF redox state at ∼5 and ∼20 min, suggesting glucose metabolism immediately increases ETC activity and later decreases fatty acid oxidation. Our results demonstrate the utility of ETF imaging in characterizing fatty acid-induced redox responses with high subcellular and temporal resolution. Our results further demonstrate a dominant role of glucose metabolism over fatty acid oxidation in β-cells even when presented with a mixed nutrient condition associated with glucolipotoxicity.

Mechanism of superoxide and hydrogen peroxide generation by human electron-transfer flavoprotein and pathological variants.

PubMed

Rodrigues, João V; Gomes, Cláudio M

2012-07-01

Reactive oxygen species production by mitochondrial enzymes plays a fundamental role both in cellular signaling and in the progression of dysfunctional states. However, sources of reactive oxygen species and the mechanisms by which enzymes produce these reactive species still remain elusive. We characterized the generation of reactive oxygen species by purified human electron-transfer flavoprotein (ETF), a mitochondrial enzyme that has a central role in the metabolism of lipids, amino acids, and choline. The results showed that ETF produces significant amounts of both superoxide and hydrogen peroxide in the presence of its partner enzyme medium-chain acyl-CoA dehydrogenase (MCAD). ETF-mediated production of reactive oxygen species is partially inhibited at high MCAD/ETF ratios, whereas it is enhanced at high ionic strength. Determination of the reduction potentials of ETF showed that thermodynamic properties of the FAD cofactor are changed upon formation of a complex between ETF and MCAD, supporting the notion that protein:protein interactions modulate the reactivity of the protein with dioxygen. Two pathogenic ETF variants were also studied to determine which factors modulate the reactivity toward molecular oxygen and promote reactive oxygen species production. The results obtained show that destabilized conformations and defective protein:protein interactions increase the ability of ETF to generate reactive oxygen species. A possible role for these processes in mitochondrial dysfunction in metabolic disorders of fatty acid β-oxidation is discussed. Copyright © 2012 Elsevier Inc. All rights reserved.

ETFDH mutations as a major cause of riboflavin-responsive multiple acyl-CoA dehydrogenation deficiency.

PubMed

Olsen, Rikke K J; Olpin, Simon E; Andresen, Brage S; Miedzybrodzka, Zofia H; Pourfarzam, Morteza; Merinero, Begoña; Frerman, Frank E; Beresford, Michael W; Dean, John C S; Cornelius, Nanna; Andersen, Oluf; Oldfors, Anders; Holme, Elisabeth; Gregersen, Niels; Turnbull, Douglass M; Morris, Andrew A M

2007-08-01

Multiple acyl-CoA dehydrogenation deficiency (MADD) is a disorder of fatty acid, amino acid and choline metabolism that can result from defects in two flavoproteins, electron transfer flavoprotein (ETF) or ETF: ubiquinone oxidoreductase (ETF:QO). Some patients respond to pharmacological doses of riboflavin. It is unknown whether these patients have defects in the flavoproteins themselves or defects in the formation of the cofactor, FAD, from riboflavin. We report 15 patients from 11 pedigrees. All the index cases presented with encephalopathy or muscle weakness or a combination of these symptoms; several had previously suffered cyclical vomiting. Urine organic acid and plasma acyl-carnitine profiles indicated MADD. Clinical and biochemical parameters were either totally or partly corrected after riboflavin treatment. All patients had mutations in the gene for ETF:QO. In one patient, we show that the ETF:QO mutations are associated with a riboflavin-sensitive impairment of ETF:QO activity. This patient also had partial deficiencies of flavin-dependent acyl-CoA dehydrogenases and respiratory chain complexes, most of which were restored to control levels after riboflavin treatment. Low activities of mitochondrial flavoproteins or respiratory chain complexes have been reported previously in two of our patients with ETF:QO mutations. We postulate that riboflavin-responsive MADD may result from defects of ETF:QO combined with general mitochondrial dysfunction. This is the largest collection of riboflavin-responsive MADD patients ever reported, and the first demonstration of the molecular genetic basis for the disorder.

77 FR 75200 - AllianceBernstein Active ETFs, Inc., et al.; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-19

... SECURITIES AND EXCHANGE COMMISSION [Investment Company Act Release No. 30305; 812-13797] AllianceBernstein Active ETFs, Inc., et al.; Notice of Application December 13, 2012. AGENCY: Securities and...Bernstein Active ETFs, Inc. (``Corporation''), AllianceBernstein L.P. (``Adviser''), and ALPS Distributors...

78 FR 65720 - Cambria ETF Trust, et al.; Notice of Application

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2013-11-01

...] Cambria ETF Trust, et al.; Notice of Application October 28, 2013. AGENCY: Securities and Exchange... (e) certain registered management investment companies and unit investment trusts outside of the same group of investment companies as the series to acquire Shares. Applicants: Cambria ETF Trust (``Trust...

78 FR 47440 - ERNY Financial ETF Trust, et al.; Notice of Application

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78 FR 14367 - Market Vectors ETF Trust, et al.; Notice of Application

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2013-03-05

...] Market Vectors ETF Trust, et al.; Notice of Application February 27, 2013. AGENCY: Securities and... companies and unit investment trusts outside of the same group of investment companies as the series to...-feeder structure. Applicants: Market Vectors ETF Trust (the ``Trust''), Van Eck Associates Corporation...

Human Factors and Safety Evaluation of the Automatic Test and Repair System (AN/MSM-105(V)1)

DTIC Science & Technology

1984-07-01

box and the main breaker box In both the ETF and ERF did not conform to military standards In that they consisted of black letters on a gold ...transportable test and repair system for electronic equipment that consists of an electronic test facility ( ETF ) and an electronic repair facility (ERF...personal gear in both the ETF and the ERF, and in the ETF there was not nearly enough room for the storage of the interconnect devices, tapes and manuals

Surface damage studies of ETFE polymer bombarded with low energy Si ions (⩽100 keV)

NASA Astrophysics Data System (ADS)

Minamisawa, Renato Amaral; Almeida, Adelaide De; Budak, Satilmis; Abidzina, Volha; Ila, Daryush

2007-08-01

Surface studies of ethylenetetrafluoroethylene (ETFE), bombarded with Si in a high-energy tandem Pelletron accelerator, have recently been reported. Si ion bombardment with a few MeV to a few hundred keV energies was shown to be sufficient to produce damage on ETFE film. We report here the use of a low energy implanter with Si ion energies lower than 100 keV, to induce changes on ETFE films. In order to determine the radiation damage, ETFE bombarded films were simulated with SRIM software and analyzed with optical absorption photometry (OAP), Raman and Fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy to show quantitatively the physical and chemical property changes. Carbonization occurs following higher dose implantation, and hydroperoxides were formed following dehydroflorination of the polymer.

Equiangular tight frames and unistochastic matrices

NASA Astrophysics Data System (ADS)

Goyeneche, Dardo; Turek, Ondřej

2017-06-01

We demonstrate that a complex equiangular tight frame composed of N vectors in dimension d, denoted ETF (d, N), exists if and only if a certain bistochastic matrix, univocally determined by N and d, belongs to a special class of unistochastic matrices. This connection allows us to find new complex ETFs in infinitely many dimensions and to derive a method to introduce non-trivial free parameters in ETFs. We present an explicit six-parametric family of complex ETF(6,16), which defines a family of symmetric POVMs. Minimal and maximal possible average entanglement of the vectors within this qubit-qutrit family are described. Furthermore, we propose an efficient numerical procedure to compute the unitary matrix underlying a unistochastic matrix, which we apply to find all existing classes of complex ETFs containing up to 20 vectors.

The mitochondrial electron transfer flavoprotein complex is essential for survival of Arabidopsis in extended darkness.

PubMed

Ishizaki, Kimitsune; Schauer, Nicolas; Larson, Tony R; Graham, Ian A; Fernie, Alisdair R; Leaver, Christopher J

2006-09-01

In mammals, the electron transfer flavoprotein (ETF) is a heterodimeric protein composed of two subunits, alpha and beta, that is responsible for the oxidation of at least nine mitochondrial matrix flavoprotein dehydrogenases. Electrons accepted by ETF are further transferred to the main respiratory chain via the ETF ubiquinone oxide reductase (ETFQO). Sequence analysis of the unique Arabidopsis homologues of two subunits of ETF revealed their high similarity to both subunits of the mammalian ETF. Yeast two-hybrid experiments showed that the Arabidopsis ETFalpha and ETFbeta can form a heteromeric protein. Isolation and characterization of two independent T-DNA insertional Arabidopsis mutants of the ETFbeta gene revealed accelerated senescence and early death compared to wild-type during extended darkness. Furthermore in contrast to wild-type, the etfb mutants demonstrated a significant accumulation of several amino acids, isovaleryl CoA and phytanoyl CoA during dark-induced carbohydrate deprivation. These phenotypic characteristics of etfb mutants are broadly similar to those that we observed previously in Arabidopsis etfqo mutants, suggesting functional association between ETF and ETFQO in Arabidopsis, and confirming the essential roles of the ETF/ETFQO electron transfer complex in the catabolism of leucine and involvement in the chlorophyll degradation pathway activated during dark-induced carbohydrate deprivation.

Sensitivity and Specificity of Eustachian Tube Function Tests in Adults

PubMed Central

Doyle, William J.; Swarts, J. Douglas; Banks, Julianne; Casselbrant, Margaretha L; Mandel, Ellen M; Alper, Cuneyt M.

2013-01-01

Objective Determine if Eustachian Tube (ET) function (ETF) tests can identify ears with physician-diagnosed ET dysfunction (ETD) in a mixed population at high sensitivity and specificity and define the inter-relatedness of ETF test parameters. Methods ETF was evaluated using the Forced-Response, Inflation-Deflation, Valsalva and Sniffing tests in 15 control ears of adult subjects after unilateral myringotomy (Group I) and in 23 ears of 19 adult subjects with ventilation tubes inserted for ETD (Group II). Data were analyzed using logistic regression including each parameter independently and then a step-down Discriminant Analysis including all ETF test parameters to predict group assignment. Factor Analysis operating over all parameters was used to explore relatedness. Results The Discriminant Analysis identified 4 ETF test parameters (Valsalva, ET opening pressure, dilatory efficiency and % positive pressure equilibrated) that together correctly assigned ears to Group II at a sensitivity of 95% and a specificity of 83%. Individual parameters representing the efficiency of ET opening during swallowing showed moderately accurate assignments of ears to their respective groups. Three factors captured approximately 98% of the variance among parameters, the first had negative loadings of the ETF structural parameters, the second had positive loadings of the muscle-assisted ET opening parameters and the third had negative loadings of the muscle-assisted ET opening parameters and positive loadings of the structural parameters. Discussion These results show that ETF tests can correctly assign individual ears to physician-diagnosed ETD with high sensitivity and specificity and that ETF test parameters can be grouped into structural-functional categories. PMID:23868429

Peptide Nucleic Acid Knockdown and Intra-host Cell Complementation of Ehrlichia Type IV Secretion System Effector.

PubMed

Sharma, Pratibha; Teymournejad, Omid; Rikihisa, Yasuko

2017-01-01

Survival of Ehrlichia chaffeensis depends on obligatory intracellular infection. One of the barriers to E. chaffeensis research progress has been the inability, using conventional techniques, to generate knock-out mutants for genes essential for intracellular infection. This study examined the use of Peptide Nucleic Acids (PNAs) technology to interrupt type IV secretion system (T4SS) effector protein expression in E. chaffeensis followed by intracellular complementation of the effector to determine its requirement for infection. Successful E. chaffeensis infection depends on the E. chaffeensis -specific T4SS protein effector, ehrlichial translocated factor-1 (Etf-1), which induces Rab5-regulated autophagy to provide host cytosolic nutrients required for E. chaffeensis proliferation. Etf-1 is also imported by host cell mitochondria where it inhibits host cell apoptosis to prolong its infection. We designed a PNA specific to Etf-1 and showed that the PNA bound to the target region of single-stranded Etf-1 RNA using a competitive binding assay. Electroporation of E. chaffeensis with this PNA significantly reduced Etf-1 mRNA and protein, and the bacteria's ability to induce host cell autophagy and infect host cells. Etf-1 PNA-mediated inhibition of ehrlichial Etf-1 expression and E. chaffeensis infection could be intracellularly trans-complemented by ectopic expression of Etf-1-GFP in host cells. These data affirmed the critical role of bacterial T4SS effector in host cell autophagy and E. chaffeensis infection, and demonstrated the use of PNA to analyze the gene functions of obligate intracellular bacteria.

Peptide Nucleic Acid Knockdown and Intra-host Cell Complementation of Ehrlichia Type IV Secretion System Effector

PubMed Central

Sharma, Pratibha; Teymournejad, Omid; Rikihisa, Yasuko

2017-01-01

Survival of Ehrlichia chaffeensis depends on obligatory intracellular infection. One of the barriers to E. chaffeensis research progress has been the inability, using conventional techniques, to generate knock-out mutants for genes essential for intracellular infection. This study examined the use of Peptide Nucleic Acids (PNAs) technology to interrupt type IV secretion system (T4SS) effector protein expression in E. chaffeensis followed by intracellular complementation of the effector to determine its requirement for infection. Successful E. chaffeensis infection depends on the E. chaffeensis-specific T4SS protein effector, ehrlichial translocated factor-1 (Etf-1), which induces Rab5-regulated autophagy to provide host cytosolic nutrients required for E. chaffeensis proliferation. Etf-1 is also imported by host cell mitochondria where it inhibits host cell apoptosis to prolong its infection. We designed a PNA specific to Etf-1 and showed that the PNA bound to the target region of single-stranded Etf-1 RNA using a competitive binding assay. Electroporation of E. chaffeensis with this PNA significantly reduced Etf-1 mRNA and protein, and the bacteria's ability to induce host cell autophagy and infect host cells. Etf-1 PNA-mediated inhibition of ehrlichial Etf-1 expression and E. chaffeensis infection could be intracellularly trans-complemented by ectopic expression of Etf-1-GFP in host cells. These data affirmed the critical role of bacterial T4SS effector in host cell autophagy and E. chaffeensis infection, and demonstrated the use of PNA to analyze the gene functions of obligate intracellular bacteria. PMID:28638803

76 FR 72483 - Self-Regulatory Organizations; Options Clearing Corporation; Order Approving Proposed Rule Change...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-23

... existing interpretation, which relates to the treatment and clearing of options on the CBOE Gold ETF... jurisdictional status CBOE Gold ETF Volatility Index and clarifies that OCC will clear and treat as securities... relative performance index is an ETF designed to measure the return of gold [[Page 72484

Molecular characterization of variant alpha-subunit of electron transfer flavoprotein in three patients with glutaric acidemia type II--and identification of glycine substitution for valine-157 in the sequence of the precursor, producing an unstable mature protein in a patient.

PubMed Central

Indo, Y; Glassberg, R; Yokota, I; Tanaka, K

1991-01-01

In our previous study of eight glutaric acidemia type II (GAII) fibroblast lines by using [35S]methionine labeling and immunoprecipitation, three of them had a defect in the synthesis of the alpha-subunit of electron transfer flavoprotein (alpha-ETF) (Ikeda et al. 1986). In one of them (YH1313) the labeling of the mature alpha-ETF was barely detectable, while that of the precursor (p) was stronger. In another (YH605) no synthesis of immunoreactive p alpha-ETF was detectable. In the third cell line (YH1391) the rate of variant p alpha-ETF synthesis was comparable to normal, but its electrophoretic mobility was slightly faster than normal. In the present study, the northern blot analysis revealed that all three mutant cell lines contained p alpha-ETF mRNA and that their size and amount were comparable to normal. In immunoblot analysis, both alpha- and beta-ETF bands were barely detectable in YH1313 and YH605 but were detectable in YH1391 in amounts comparable to normal. Sequencing of YH1313 p alpha-ETF cDNA via PCR identified a transversion of T-470 to G. We then devised a simple PCR method for the 119-bp section (T-443/G-561) for detecting this mutation. In the upstream primer, A-466 was artificially replaced with C, to introduce a BstNI site into the amplified copies in the presence of G-470 from the variant sequence. The genomic DNA analysis using this method demonstrated that YH1313 was homozygous for T----G-470 transversion. It was not detected either in two other alpha-ETF-deficient GAII or in seven control cell lines. The alpha-ETF cDNA sequence in YH605 was identical to normal. Images Figure 1 Figure 2 Figure 3 Figure 5 PMID:1882842

Electron transfer flavoprotein domain II orientation monitored using double electron-electron resonance between an enzymatically reduced, native FAD cofactor, and spin labels.

PubMed

Swanson, Michael A; Kathirvelu, Velavan; Majtan, Tomas; Frerman, Frank E; Eaton, Gareth R; Eaton, Sandra S

2011-03-01

Human electron transfer flavoprotein (ETF) is a soluble mitochondrial heterodimeric flavoprotein that links fatty acid β-oxidation to the main respiratory chain. The crystal structure of human ETF bound to medium chain acyl-CoA dehydrogenase indicates that the flavin adenine dinucleotide (FAD) domain (αII) is mobile, which permits more rapid electron transfer with donors and acceptors by providing closer access to the flavin and allows ETF to accept electrons from at least 10 different flavoprotein dehydrogenases. Sequence homology is high and low-angle X-ray scattering is identical for Paracoccus denitrificans (P. denitrificans) and human ETF. To characterize the orientations of the αII domain of P. denitrificans ETF, distances between enzymatically reduced FAD and spin labels in the three structural domains were measured by double electron-electron resonance (DEER) at X- and Q-bands. An FAD to spin label distance of 2.8 ± 0.15 nm for the label in the FAD-containing αII domain (A210C) agreed with estimates from the crystal structure (3.0 nm), molecular dynamics simulations (2.7 nm), and rotamer library analysis (2.8 nm). Distances between the reduced FAD and labels in αI (A43C) were between 4.0 and 4.5 ± 0.35 nm and for βIII (A111C) the distance was 4.3 ± 0.15 nm. These values were intermediate between estimates from the crystal structure of P. denitrificans ETF and a homology model based on substrate-bound human ETF. These distances suggest that the αII domain adopts orientations in solution that are intermediate between those which are observed in the crystal structures of free ETF (closed) and ETF bound to a dehydrogenase (open). Copyright © 2011 The Protein Society.

Predictors of Enteral Tube Feeding in Hospitalized Older Adults.

PubMed

Crenitte, Milton Roberto Furst; Avelino-Silva, Thiago Junqueira; Apolinario, Daniel; Curiati, Jose Antonio Esper; Campora, Flavia; Jacob-Filho, Wilson

2017-11-01

Despite general recognition that enteral tube feeding (ETF) is frequently employed in long-term care facilities and patients with dementia, remarkably little research has determined which factors are associated with its use in acutely ill older adults. In this study, we aimed to investigate determinants of ETF introduction in hospitalized older adults. We examined a retrospective cohort of acutely ill patients, aged 60 years and older, admitted to a university hospital's geriatric ward from 2014-2015, in São Paulo, Brazil. The main outcome was the introduction of ETF during hospitalization. Predictors of interest included age, sex, referring unit, comorbidity burden, functional status, malnutrition, depression, dementia severity, and delirium. Multivariate analysis was performed using backward stepwise logistic regression. A total of 214 cases were included. Mean age was 81 years, and 63% were women. Malnutrition was detected in 47% of the cases, dementia in 46%, and delirium in 36%. ETF was initiated in 44 (21%) admissions. Independent predictors of ETF were delirium (odds ratio [OR], 4.83; 95% CI, 2.12-11.01; P < .001) and total functional dependency (OR, 8.95; 95% CI, 2.87-27.88; P < .001). Malnutrition was not independently associated with ETF. One in five acutely ill older adults used ETF while hospitalized. Delirium and functional dependency were independent predictors of its introduction. Risks and benefits of enteral nutrition in this particular context need to be further explored.

2009 EVALUATION OF TRITIUM REMOVAL AND MITIGATION TECHNOLOGIES FOR WASTEWATER TREATMENT

DOE Office of Scientific and Technical Information (OSTI.GOV)

LUECK KJ; GENESSE DJ; STEGEN GE

2009-02-26

Since 1995, a state-approved land disposal site (SALDS) has received tritium contaminated effluents from the Hanford Site Effluent Treatment Facility (ETF). Tritium in this effluent is mitigated by storage in slow moving groundwater to allow extended time for decay before the water reaches the site boundary. By this method, tritium in the SALDS is isolated from the general environment and human contact until it has decayed to acceptable levels. This report contains the 2009 update evaluation of alternative tritium mitigation techniques to control tritium in liquid effluents and groundwater at the Hanford site. A thorough literature review was completed andmore » updated information is provided on state-of-the-art technologies for control of tritium in wastewaters. This report was prepared to satisfy the Hanford Federal Facility Agreement and Consent Order (Tri-Party Agreement) Milestone M-026-07B (Ecology, EPA, and DOE 2007). Tritium separation and isolation technologies are evaluated periodically to determine their feasibility for implementation to control Hanford site liquid effluents and groundwaters to meet the Us. Code of Federal Regulations (CFR), Title 40 CFR 141.16, drinking water maximum contaminant level (MCL) for tritium of 20,000 pOll and/or DOE Order 5400.5 as low as reasonably achievable (ALARA) policy. Since the 2004 evaluation, there have been a number of developments related to tritium separation and control with potential application in mitigating tritium contaminated wastewater. These are primarily focused in the areas of: (1) tritium recycling at a commercial facility in Cardiff, UK using integrated tritium separation technologies (water distillation, palladium membrane reactor, liquid phase catalytic exchange, thermal diffusion), (2) development and demonstration of Combined Electrolysis Catalytic Exchange (CECE) using hydrogen/water exchange to separate tritium from water, (3) evaporation of tritium contaminated water for dispersion in the atmosphere, and (4) use of barriers to minimize the transport of tritium in groundwater. Continuing development efforts for tritium separations processes are primarily to support the International Thermonuclear Experimental Reactor (ITER) program, the nuclear power industry, and the production of radiochemicals. While these applications are significantly different than the Hanford application, the technology could potentially be adapted for Hanford wastewater treatment. Separations based processes to reduce tritium levels below the drinking water MCL have not been demonstrated for the scale and conditions required for treating Hanford wastewater. In addition, available cost information indicates treatment costs for such processes will be substantially higher than for discharge to SALDS or other typical pump and treat projects at Hanford. Actual mitigation projects for groundwater with very low tritium contamination similar to that found at Hanford have focused mainly on controlling migration and on evaporation for dispersion in the atmosphere.« less

76 FR 6648 - Self-Regulatory Organizations; The Options Clearing Corporation; Notice of Filing of Proposed...

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... accommodate the clearance of options on certain indexes measuring the relative performance of one reference... performance of one reference security or reference index relative to a second reference security or reference..., an Alpha Index may measure the relative total return of two non-ETF securities, two ETFs, or one ETF...

75 FR 39074 - Self-Regulatory Organizations; EDGA Exchange, Inc.; Notice of Filing of Proposed Rule Change To...

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2010-07-07

... but not in the stocks that underlie that ETF, what consequences might that have for the underlying stocks or other products? What are the potential effects on price discovery for the ETF, the underlying..., ETPs include Exchange Traded Funds (``ETF''),\\3\\ Exchange Traded Vehicles (``ETV''),\\4\\ and Exchange...

75 FR 39078 - Self-Regulatory Organizations; Chicago Board Options Exchange, Incorporated; Notice of Filing of...

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2010-07-07

... index? If there are trading pauses in an ETF but not in the stocks that underlie that ETF, what... price discovery for the ETF, the underlying stocks and other products? Are there other market-based... Change Related to Individual Stock Trading Pauses Due to Extraordinary Market Volatility June 30, 2010...

76 FR 2931 - Self-Regulatory Organizations; Notice of Filing and Immediate Effectiveness of Proposed Rule...

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2011-01-18

... open additional series of stock options or ETF options under certain circumstances. The proposed change... series for each class of stock options or ETF options open for trading on the Exchange; and that the Exchange may open additional series of stock options or ETF options under certain circumstances. The...

78 FR 21443 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-10

... (``ETF'') option (as discussed below, excluding mini options) ADV, as reported by the Options Clearing... Exchange notes that Equity and ETF Customer volume is a widely followed benchmark of industry volume and is... product categories: equity and ETF volume and index volume, and the information can be filtered to show...

Average Nuclear Potentials from Selfconsistent Semiclassical Calculations

NASA Astrophysics Data System (ADS)

Bartel, J.

1999-03-01

Using the selfconsistent semiclassical Extended Thomas-Fermi (ETF) method up to 4th order in connection with Skyrme forces it is demonstrated that the neutron and proton average potentials obtained using the semiclassical functionals τ (ETF)[ρ] and vec {J}(ETF)[ρ] reproduce the corresponding Hartree-Fock fields extremely well, except for shell oscillations in the nuclear center.

77 FR 24233 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

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2012-04-23

... Change Relating to the Peritus High Yield ETF April 17, 2012. Pursuant to Section 19(b)(1) of the... Change The Exchange proposes to reflect a change to the holdings of the Peritus High Yield ETF to achieve... Exchange shares (``Shares'') of the Peritus High Yield ETF (``Fund'') under [[Page 24234

78 FR 54715 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-05

... Change Relating to Investments in Leveraged Loans by the Peritus High Yield ETF August 29, 2013. Pursuant... the holdings of the Peritus High Yield ETF to achieve its investment objective to include leveraged loans. Peritus High Yield ETF is currently listed and traded on the Exchange under NYSE Arca Equities...

Enteral tube feeding in adults with cystic fibrosis; patient choice and impact on long term outcomes.

PubMed

White, H; Morton, A M; Conway, S P; Peckham, D G

2013-12-01

Enteral tube feeding (ETF) has been evaluated in paediatric and mixed child and adult populations with cystic fibrosis, demonstrating positive outcomes from 6 months to 2 years post insertion. No studies have examined the longer term nutritional and clinical outcomes in an exclusively adult population with cystic fibrosis or compared the outcomes for those who meet standard criteria and opt to undertake or decline ETF. Twenty three out of 380 patients attending the Leeds Regional Adult CF unit fulfilled the standard criteria for commencing ETF (CF Trust, 2002) between 2004 and 2008. Weight, BMI, FEV1, FVC, CFRD, and number of intravenous antibiotic treatment days were collected at 1 year pre baseline, at baseline, and at 1, 2, and 3 years post baseline for all these patients whether they accepted or declined ETF. Seventeen of the 23 patients agreed to accept a programme of ETF, two of whom died within the first year of ETF. In the remaining patients (n=15), weight increased by 19.5% from baseline (p<0.001), BMI increased to within the normal range and lung function stabilised. There was no reduction in the requirement for intravenous antibiotic treatment. The six patients who declined ETF had a decline in lung function and no weight gain. Supplemental enteral tube feeding improves clinical outcomes when administered over 3 years, resulting in significant weight gain, a normal BMI and stabilisation of lung function. It does not reduce intravenous antibiotic treatment days. In contrast those patients eligible for, but who declined ETF, showed a deterioration in lung function and a failure to gain weight and to achieve normal BMI status. Copyright © 2013 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

cDNA cloning, functional expression and cellular localization of rat liver mitochondrial electron-transfer flavoprotein-ubiquinone oxidoreductase protein.

PubMed

Huang, Shengbing; Song, Wei; Lin, Qishui

2005-08-01

A membrane-bound protein was purified from rat liver mitochondria. After being digested with V8 protease, two peptides containing identical 14 amino acid residue sequences were obtained. Using the 14 amino acid peptide derived DNA sequence as gene specific primer, the cDNA of correspondent gene 5'-terminal and 3'-terminal were obtained by RACE technique. The full-length cDNA that encoded a protein of 616 amino acids was thus cloned, which included the above mentioned peptide sequence. The full length cDNA was highly homologous to that of human ETF-QO, indicating that it may be the cDNA of rat ETF-QO. ETF-QO is an iron sulfur protein located in mitochondria inner membrane containing two kinds of redox center: FAD and [4Fe-4S] center. After comparing the sequence from the cDNA of the 616 amino acids protein with that of the mature protein of rat liver mitochondria, it was found that the N terminal 32 amino acid residues did not exist in the mature protein, indicating that the cDNA was that of ETF-QOp. When the cDNA was expressed in Saccharomyces cerevisiae with inducible vectors, the protein product was enriched in mitochondrial fraction and exhibited electron transfer activity (NBT reductase activity) of ETF-QO. Results demonstrated that the 32 amino acid peptide was a mitochondrial targeting peptide, and both FAD and iron-sulfur cluster were inserted properly into the expressed ETF-QO. ETF-QO had a high level expression in rat heart, liver and kidney. The fusion protein of GFP-ETF-QO co-localized with mitochondria in COS-7 cells.

Magnetohydrodynamics (MHD) Engineering Test Facility (ETF) 200 MWe power plant. Conceptual Design Engineering Report (CDER) supplement. Magnet system special investigations

NASA Technical Reports Server (NTRS)

1981-01-01

The results of magnet system special investigations listed below are summarized: 4 Tesla Magnet Alternate Design Study; 6 Tesla Magnet Manufacturability Study. The conceptual design for a 4 Tesla superconducting magnet system for use with an alternate (supersonic) ETF power train is described, and estimated schedule and cost are identified. The magnet design is scaled from the ETF 6 T Tesla design. Results of a manufacturability study and a revised schedule and cost estimate for the ETF 6 T magnet are reported. Both investigations are extensions of the conceptual design of a 6 T magnet system performed earlier as a part of the overall MED-ETF conceptual design described in Conceptual Design Engineering Report (CDER) Vol. V, System Design Description (SDD) 503 dated September, 1981, DOE/NASA/0224-1; NASA CR-165/52.

Approximate equiangular tight frames for compressed sensing and CDMA applications

NASA Astrophysics Data System (ADS)

Tsiligianni, Evaggelia; Kondi, Lisimachos P.; Katsaggelos, Aggelos K.

2017-12-01

Performance guarantees for recovery algorithms employed in sparse representations, and compressed sensing highlights the importance of incoherence. Optimal bounds of incoherence are attained by equiangular unit norm tight frames (ETFs). Although ETFs are important in many applications, they do not exist for all dimensions, while their construction has been proven extremely difficult. In this paper, we construct frames that are close to ETFs. According to results from frame and graph theory, the existence of an ETF depends on the existence of its signature matrix, that is, a symmetric matrix with certain structure and spectrum consisting of two distinct eigenvalues. We view the construction of a signature matrix as an inverse eigenvalue problem and propose a method that produces frames of any dimensions that are close to ETFs. Due to the achieved equiangularity property, the so obtained frames can be employed as spreading sequences in synchronous code-division multiple access (s-CDMA) systems, besides compressed sensing.

75 FR 11221 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing and Immediate Effectiveness of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-10

... traded either on the OTC Bulletin Board or the Pink OTC Markets. The Exchange represents that: (1) Except... Change Relating to Listing of Wilshire 5000 Total Market ETF and the Wilshire 4500 Completion ETF March 3... proposes to list and trade the shares of the Wilshire 5000 Total Market ETF and the Wilshire 4500...

75 FR 39086 - Self-Regulatory Organizations; Notice of Filing of a Proposed Rule Change by NASDAQ OMX BX, Inc...

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2010-07-07

... but not in the stocks that underlie that ETF, what consequences might that have for the underlying stocks or other products? What are the potential effects on price discovery for the ETF, the underlying... Vehicles (``ETVs''),\\4\\ and Exchange Traded Notes (``ETNs'').\\5\\ \\3\\ An ETF is an open-ended registered...

76 FR 5848 - Self-Regulatory Organizations; NASDAQ OMX PHLX LLC; Notice of Filing and Immediate Effectiveness...

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2011-02-02

... component is the underlying stock or ETF to the functionality on its electronic trading platform for options... or sell a stated number of units of an underlying stock or ETF coupled with the purchase or sale of... underlying stock or Exchange Traded Fund Share (``ETF'') is also referred to as a stock-option order. 2...

75 FR 39065 - Self-Regulatory Organizations; Chicago Stock Exchange, Inc.; Notice of Filing of Proposed Rule...

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2010-07-07

... ETF but not in the stocks that underlie that ETF, what consequences might that have for the underlying stocks or other products? What are the potential effects on price discovery for the ETF, the underlying... Organizations; Chicago Stock Exchange, Inc.; Notice of Filing of Proposed Rule Change To Amend the List of...

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Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-07

... products based on that index? If there are trading pauses in an ETF but not in the stocks that underlie that ETF, what consequences might that have for the underlying stocks or other products? What are the potential effects on price discovery for the ETF, the underlying stocks and other products? Are there other...

Vocational Education for Sustainable Development: An Obligation for the European Training Foundation

ERIC Educational Resources Information Center

Viertel, Evelyn

2010-01-01

This article presents an argued case for making sustainable development a policy obligation for the European Training Foundation (ETF) and for vocational education and training (VET) reform in the partner countries that are served by ETF. In relation to ETF it points out that no major emphasis has been put to date on combining economic, social and…

77 FR 65920 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing of Proposed Rule Change Relating...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-31

... Trading of Shares of the Pring Turner Business Cycle ETF Under NYSE Arca Equities Rule 8.600 October 25... Turner Business Cycle ETF. The text of the proposed rule change is available on the Exchange's Web site... and trade shares (``Shares'') of the Pring Turner Business Cycle ETF (``Fund'') under NYSE Arca...

Wavelength Dependence of Excimer Laser Irradiation Effects on Ethylene-Tetrafluoroethylene Copolymer

NASA Astrophysics Data System (ADS)

Hamada, Yuji; Kawanishi, Shunichi; Nishii, Masanobu; Sugimoto, Shun'ichi; Yamamoto, Tadashi

1994-08-01

Irradiation with an ArF laser at wavelength of 193 nm formed diene in a whole ethylene-tetrafluoroethylene copolymer (ETFE) film and irradiation with a KrF and a XeCl laser at 248 and 308 nm induced the carbonization of ETFE. ArF-laser radiation at 193 nm formed diene in the bulk of ETFE via the process of single-photon absorption, and in case of KrF and XeCl-laser irradiation multiphoton absorption brought about the carbonization of ETFE. The surface analysis by X-ray photoelectron spectroscopy showed that excimer laser-induced elimination of fluorine atoms depended on the laser wavelength.

Complex equiangular tight frames

NASA Astrophysics Data System (ADS)

Tropp, Joel A.

2005-08-01

A complex equiangular tight frame (ETF) is a tight frame consisting of N unit vectors in Cd whose absolute inner products are identical. One may view complex ETFs as a natural geometric generalization of an orthonormal basis. Numerical evidence suggests that these objects do not arise for most pairs (d, N). The goal of this paper is to develop conditions on (d, N) under which complex ETFs can exist. In particular, this work concentrates on the class of harmonic ETFs, in which the components of the frame vectors are roots of unity. In this case, it is possible to leverage field theory to obtain stringent restrictions on the possible values for (d, N).

Tympanometric changes and eustachian tube function in patients with hypothyroidism.

PubMed

Orhan, Israfil; Palit, Fatih; Aydin, Salih; Soylu, Erkan; Sakallioglu, Oner

2014-05-01

The aim of this study was to investigate the tympanometric changes and eustachian tube function (ETF) in patients with hypothyroidism. Automatic ETF tests were performed and tympanometric measurements were evaluated to assess ETF in 40 patients diagnosed with hypothyroidism and a 40-patient euthyroid control group. Levothyroxine sodium tablet treatment was started in patients with hypothyroidism. After achieving a euthyroid state in these patients, the tympanometric measurements and automatic ETF tests were repeated. When the patient groups (hypothyroid and control) were compared in terms of ETF, a statistically significant ET dysfunction was observed in the hypothyroid patient group (P < 0.01).When hypothyroid patients were evaluated in terms of ETF before and after treatment, whereas 61.3% of cases had ET function before treatment, this ratio increased to 78.8% after treatment. Furthermore, according to pressure and compliance measurements, statistically significant increases were found in the after treatment measurements (P < 0.05). As a result of this study, we have come to the opinion that hypothyroidism can change tympanometric measurements and also cause ET dysfunction. However, more comprehensive and detailed studies researching the effects of hypothyroidism on tympanometric measurements are needed.

DEER distance measurement between a spin label and a native FAD semiquinone in electron transfer flavoprotein.

PubMed

Swanson, Michael A; Kathirvelu, Velavan; Majtan, Tomas; Frerman, Frank E; Eaton, Gareth R; Eaton, Sandra S

2009-11-11

The human mitochondrial electron transfer flavoprotein (ETF) accepts electrons from at least 10 different flavoprotein dehydrogenases and transfers electrons to a single electron acceptor in the inner membrane. Paracoccus denitrificans ETF has the identical function, shares the same three-dimensional structure and functional domains, and exhibits the same conformational mobility. It has been proposed that the mobility of the alphaII domain permits the promiscuous behavior of ETF with respect to a variety of redox partners. Double electron-electron resonance (DEER) measurements between a spin label and an enzymatically reduced flavin adenine dinucleotide (FAD) cofactor in P. denitrificans ETF gave two distributions of distances: a major component centered at 4.2 +/- 0.1 nm and a minor component centered at 5.1 +/- 0.2 nm. Both components had widths of approximately 0.3 nm. A distance of 4.1 nm was calculated using the crystal structure of P. denitrificans ETF, which agrees with the major component obtained from the DEER measurement. The observation of a second distribution suggests that ETF, in the absence of substrate, adopts some conformations that are intermediate between the predominant free and substrate-bound states.

DEER Distance Measurement Between a Spin Label and a Native FAD Semiquinone in Electron Transfer Flavoprotein

PubMed Central

Swanson, Michael A.; Kathirvelu, Velavan; Majtan, Tomas; Frerman, Frank E.; Eaton, Gareth R.; Eaton, Sandra S.

2009-01-01

The human mitochondrial electron transfer flavoprotein (ETF) accepts electrons from at least 10 different flavoprotein dehydrogenases and transfers electrons to a single electron acceptor in the inner membrane. Paracoccus denitrificans ETF has the identical function, shares the same three dimensional structure and functional domains, and exhibits the same conformational mobility. It has been proposed that the mobility of the αII domain permits the promiscuous behavior of ETF with respect to a variety of redox partners. Double electron-electron resonance (DEER) measurements between a spin label and an enzymatically reduced flavin adenine dinucleotide (FAD) cofactor in P. denitrificans ETF gave two distributions of distances: a major component centered at 4.2 ± 0.1 nm and a minor component centered at 5.1 ± 0.2 nm. Both components had widths of approximately 0.3 nm. A distance of 4.1 nm was calculated using the crystal structure of P. denitrificans ETF, which agrees with the major component obtained from the DEER measurement. The observation of a second distribution suggests that ETF, in the absence of substrate, adopts some conformations that are intermediate between the predominant free and substrate-bound states. PMID:19886689

Eustachian tube function in adults without middle ear disease.

PubMed

Swarts, J Douglas; Alper, Cuneyt M; Mandel, Ellen M; Villardo, Richard; Doyle, William J

2011-04-01

We sought to develop normative values for 5 eustachian tube function (ETF) test protocols in adults without otitis media (OM). Twenty adults (19 to 48 years of age) without a recent history of OM (5 had OM in childhood) underwent unilateral myringotomy and were evaluated for ETF by use of the forced response, inflation, deflation, forcible "sniff", and Valsalva test protocols. When possible, these tests were repeated on a second day. Normative values for the parameters of these protocols in adult subjects without a recent history of OM were developed. Between-day data for the forced response test were highly correlated. A percentage of these tests showed eustachian tube "constriction" during swallowing--an abnormal condition. The percent reduction in applied pressures for the inflation and deflation tests was high, indicative of good ETF. Few subjects had a positive "sniff" test, whereas most had a positive Valsalva test, and the results for both tests were effort-dependent. Results of ETF tests in adults with and without recent OM have not been published. Normative data are now available for comparison with ETF test results in adults with OM. These protocols will be used to evaluate the efficacy of surgical procedures designed to improve ETF.

Body Weight and Body Mass Index in Patients with End-Stage Cystic Fibrosis Stabilize After the Start of Enteral Tube Feeding.

PubMed

Hollander, Francis M; de Roos, Nicole M; Belle van Meerkerk, Gerdien; Teding van Berkhout, Ferdinand; Heijerman, Harry G M; van de Graaf, Ed A

2017-11-01

Enteral tube feeding (ETF) is widely used in patients with cystic fibrosis (CF) and end-stage lung disease, but previous studies have been limited to investigating whether ETF improves outcomes in patients with moderately or mildly impaired pulmonary function. This study investigated body weight, body mass index (BMI; calculated as kg/m 2 ), pulmonary function, and the presence of CF-related diabetes before and after the start of ETF. This was a retrospective observational study. Data from 26 adult patients in an outpatient setting who had end-stage CF (19 women) and had been using ETF for at least 6 months between 2000 and 2014 were analyzed. Body weight, BMI, pulmonary function (forced expiratory volume in 1 second as percent of predicted) and incidence of CF-related diabetes from 6 months before to 6 months after starting ETF. Time effects were tested with one-way analysis of variance for data that were normally distributed and the Friedman test for non-parametric data. Correlations were tested with Pearson's r or Spearman's ρ, depending on the distribution of the data. Mean body weight increased by 3.5 kg (95% CI 2.2 to 4.8 kg) after patients started ETF. In women, mean BMI decreased by 0.7 in the 6 months before the start of ETF (P<0.05) and increased by 1.4 in the 6 months thereafter (P<0.05). In men, BMI changes were similar (-0.8 and +1.1), but not statistically significant. Forced expiratory volume in 1 second as percent of predicted significantly decreased in time from a median of 28% to 26% at the start of ETF to 25% after 6 months (P=0.0013), with similar trends in women and men. There was no correlation between changes in weight and lung function. CF-related diabetes was already present in 12 patients and developed in 1 more patient after the start of ETF. ETF improved body weight and BMI but not pulmonary function in 26 patients with end-stage CF. Clinical outcomes were similar in women and men, but the sample size of men was too small to determine statistical significance. Copyright © 2017 Academy of Nutrition and Dietetics. Published by Elsevier Inc. All rights reserved.

[The release of flavin adenine dinucleotide upon local conformational transition in electron-transferring flavoprotein induced by trimethylamine dehydrogenase].

PubMed

Lomtev, A S; Bobrov, A G; Vekshin, N L

2004-01-01

The electron-transferring proteins, trimethylamine dehydrogenase (TMAD) and electron-transferring flavoprotein (ETF) from the bacterium Methylophilius methylotrophus, were studied in vitro by fluorescence spectroscopy. Flavin adenine dinucleotide (FAD) was found to be capable of a slow and spontaneous release from ETF, which is accompanied by an increase in flavin fluorescence. At a rather high ionic strength (0.1 M NaCl or 50 mM phosphate), the FAD release is sharply activated by TMAD preparations that induce a local conformational transition in ETF. The values of tryptophan fluorescence polarization and lifetime and the use of the Levshin-Perrin equation helped show that the size of protein particles remain unchanged upon the TMAD and ETF mixing; i.e., these proteins themselves do not form a stable complex with each other. The protein mixture did not release flavin from ETF in the presence of trimethylamine and formaldehyde. In this case, a stable complex between the proteins appeared to be formed under the action of formaldehyde. Upon a short-term incubation of ETF with ferricyanide, FAD was hydrolyzed to flavin mononucleotide (FMN) and AMP. This fact explains the previous detection of AMP in ETF preparations by some researches. A fluorescence method was proposed for distinguishing FAD from FMN in solution using ethylene glycol. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 3; see also http://www.maik.ru.

A cost-utility analysis in patients receiving enteral tube feeding at home and in nursing homes.

PubMed

Elia, Marinos; Stratton, Rebecca J

2008-06-01

A cost-utility analysis was undertaken of enteral tube feeding (ETF) in patients with cerebrovascular accident (CVA). Mortality during ETF was established in nursing homes (n7007) or at home (n2888). Quality of life was measured (EuroQol) on a sub-sample (n25). Quality adjusted life years (QALYs) over 3 years and the cost/QALY were calculated. The cost/QALY (1 pound=1.482 euros; 30/06/05) for patients receiving ETF at home was 12,817 pounds (10,351 pounds-16,826 pounds using 95% CI for quality of life) and insensitive to the frequency of home visits (50-150% of the average frequency; 11,851 pounds-13,782 pounds), outcome of patients reverting to full oral feeding (0-100% survival; 11,023 pounds-14,440 pounds), and computed outcome of a 'control' group not given ETF (0.125-0.25 year survival; 12,991 pound-14,006 pounds). The cost/QALY in nursing homes (10,304 pounds-68,064 pounds) varied depending on the state contribution to non-medical costs and is above and below the threshold (30,000 pounds/QALY). The data suggest the cost-effectiveness of ETF in patients with CVA receiving ETF at home or in nursing homes, where the non-medical costs are paid privately, compares favourably with other interventions. The cost-effectiveness of ETF in nursing homes when the state pays all non-medical costs compares unfavourably with other treatments, raising economic, clinical, and ethical concerns.

Expression of sarco/endoplasmic reticulum Ca(2+) ATPase (SERCA) 3 proteins in two major conformational states in native human cell membranes.

PubMed

Corvazier, Elisabeth; Bredoux, Raymonde; Kovács, Tünde; Enouf, Jocelyne

2009-03-01

The SERCA family includes 3 genes (SERCA1-3), each of which giving rise to various isoforms. To date, detailed structural data is only available for the SERCA1a isoform. Here, limited trypsinolysis of either human platelet membranes or recombinant SERCA3a in HEK-293 cells followed by Western blotting using antibodies covering different regions of the SERCA3(a) protein revealed two, kinetically distinct, Early (ETF) and Late (LTF) Tryptic Fragmentations. The ETF uses many tryptic sites while the LTF uses a unique tryptic site. Using site-directed mutagenesis: i) Arg(334), Arg(396) and Arg(638) were directly assigned to the ETF and ii) Arg(198) was assigned as the only tryptic site to the LTF. Arg(671), Lys(712)/Lys(713) and Lys(728) were also found to modulate the ETF. SERCA inhibitors Tg and tBHQ induced modest inhibition of the ETF. In contrast, the addition of CaCl(2), EGTA or AlF(4)(-) strikingly modified the ETF without any effect on the LTF. Trypsinolysis of the other recombinant SERCA3b-3f isoforms revealed: i) same ETF and LTF as SERCA3a, with variations of the length of the C-terminal fragments; ii) Arg(1002) as an additional tryptic site in SERCA3b-3e isoforms. Taken together, the two distinct SERCA3 fragmentation profiles sign the co-expression of SERCA3 proteins in two conformational states in cell membranes.

The Iron-Sulfur Cluster of Electron Transfer Flavoprotein-ubiquinone Oxidoreductase (ETF-QO) is the Electron Acceptor for Electron Transfer Flavoprotein†

PubMed Central

Swanson, Michael A.; Usselman, Robert J.; Frerman, Frank E.; Eaton, Gareth R.; Eaton, Sandra S.

2011-01-01

Electron-transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) accepts electrons from electron-transfer flavoprotein (ETF) and reduces ubiquinone from the ubiquinone-pool. It contains one [4Fe-4S]2+,1+ and one FAD, which are diamagnetic in the isolated oxidized enzyme and can be reduced to paramagnetic forms by enzymatic donors or dithionite. In the porcine protein, threonine 367 is hydrogen bonded to N1 and O2 of the flavin ring of the FAD. The analogous site in Rhodobacter sphaeroides ETF-QO is asparagine 338. Mutations N338T and N338A were introduced into the R. sphaeroides protein by site-directed mutagenesis to determine the impact of hydrogen bonding at this site on redox potentials and activity. The mutations did not alter the optical spectra, EPR g-values, spin-lattice relaxation rates, or the [4Fe-4S]2+,1+ to FAD point-dipole interspin distances. The mutations had no impact on the reduction potential for the iron-sulfur cluster, which was monitored by changes in the continuous wave EPR signals of the [4Fe-4S]+ at 15 K. For the FAD semiquinone, significantly different potentials were obtained by monitoring the titration at 100 or 293 K. Based on spectra at 293 K the N338T mutation shifted the first and second midpoint potentials for the FAD from +47 mV and −30 mV for wild type to −11 mV and −19 mV, respectively. The N338A mutation decreased the potentials to −37 mV and −49 mV. Lowering the midpoint potentials resulted in a decrease in the quinone reductase activity and negligible impact on disproportionation of ETF1e− catalyzed by ETF-QO. These observations indicate that the FAD is involved in electron transfer to ubiquinone, but not in electron transfer from ETF to ETF-QO. Therefore the iron-sulfur cluster is the immediate acceptor from ETF. PMID:18672901

Surface Plasmon Resonance Sensor Based on Ethylene Tetra-Fluoro-Ethylene Hollow Fiber.

PubMed

Chen, Pan; He, Yu-Jing; Zhu, Xiao-Song; Shi, Yi-Wei

2015-11-03

A new kind of hollow fiber surface plasmon resonance sensor (HF-SPRS) based on the silver-coated ethylene tetra-fluoro-ethylene (ETFE) hollow fiber (HF) is presented. The ETFE HF-SPRS is fabricated, and its performance is investigated experimentally by measuring the transmission spectra of the sensor when filled by liquid sensed media with different refractive indices (RIs). Theoretical analysis based on the ray transmission model is also taken to evaluate the sensor. Because the RI of ETFE is much lower than that of fused silica (FSG), the ETFE HF-SPRS can extend the lower limit of the detection range of the early reported FSG HF-SPRS from 1.5 to 1.42 approximately. This could greatly enhance the application potential of HF-SPRS. Moreover, the joint use of both ETFE and FSG HF-SPRSs can cover a wide detection range from 1.42 to 1.69 approximately with high sensitivities larger than 1000 nm/RIU.

Temperature effect on radiation induced reactions in ethylene and tetrafluoroethylene copolymer (ETFE)

NASA Astrophysics Data System (ADS)

Oshima, Akihiro; Ikeda, Shigetoshi; Seguchi, Tadao; Tabata, Yoneho

1997-11-01

Ethylene and tetrafluoroethylene copolymer (ETFE) was irradiated by γ-rays or electron beam (EB) under oxygen-free atmosphere at various temperatures ranging from 77 to 573 K. Mechanical and thermal properties, and absorption spectra of the irradiated ETFEs were measured. The mechanical properties of the film have been observed to change by irradiation. The modulus and yield strength increase with increasing dose, and these phenomena are clearly distinguished above the melting temperature of ETFE (533 K). Heat of crystallization changes drastically as a function of irradiation dose around the melting temperature, compared with other temperatures. The absorption band around 250 nm of irradiated ETFE shifts to a longer wavelength region with increase of irradiation temperature. Therefore, it was concluded from those experimental results mentioned above that crosslinking takes place and conjugated double bonds formation proceeds in a wide range of irradiation temperatures. Those reactions are enhanced by increasing temperature. The homogeneous crosslinking takes place in the molten state, while the heterogeneous crosslinking does in the crystalline solid state.

Surface Plasmon Resonance Sensor Based on Ethylene Tetra-Fluoro-Ethylene Hollow Fiber

PubMed Central

Chen, Pan; He, Yu-Jing; Zhu, Xiao-Song; Shi, Yi-Wei

2015-01-01

A new kind of hollow fiber surface plasmon resonance sensor (HF-SPRS) based on the silver-coated ethylene tetra-fluoro-ethylene (ETFE) hollow fiber (HF) is presented. The ETFE HF-SPRS is fabricated, and its performance is investigated experimentally by measuring the transmission spectra of the sensor when filled by liquid sensed media with different refractive indices (RIs). Theoretical analysis based on the ray transmission model is also taken to evaluate the sensor. Because the RI of ETFE is much lower than that of fused silica (FSG), the ETFE HF-SPRS can extend the lower limit of the detection range of the early reported FSG HF-SPRS from 1.5 to 1.42 approximately. This could greatly enhance the application potential of HF-SPRS. Moreover, the joint use of both ETFE and FSG HF-SPRSs can cover a wide detection range from 1.42 to 1.69 approximately with high sensitivities larger than 1000 nm/RIU. PMID:26540062

Anisotropic proton-conducting membranes prepared from swift heavy ion-beam irradiated ETFE films

NASA Astrophysics Data System (ADS)

Kimura, Yosuke; Chen, Jinhua; Asano, Masaharu; Maekawa, Yasunari; Katakai, Ryoichi; Yoshida, Masaru

2007-10-01

Poly(ethylene-co-tetrafluoroethylene) (ETFE) films were irradiated by swift heavy ion-beams of 129Xe 23+ with fluences of 0, 3 × 10 6, 3 × 10 7, 3 × 10 8 and 3 × 10 9 ions/cm 2, followed by γ-ray pre-irradiation for radiation grafting of styrene onto the ETFE films and sulfonation of the grafted ETFE films to prepare highly anisotropic proton-conducting membranes. The fluence of Xe ions and the addition of water in the grafting solvent were examined to determine their effect on the proton conductivity of the resultant membranes. It was found that the polymer electrolyte membrane prepared by grafting the styrene monomer in a mixture of 67% isopropanol and 33% water to the ETFE film with an ion-beam irradiation fluence of 3.0 × 10 6 ions/cm 2 was a highly anisotropic proton-conducting material, as the proton conductivity was three or more times higher in the thickness direction than in the surface direction of the membrane.

Identification of the electron transfer flavoprotein as an upregulated enzyme in the benzoate utilization of Desulfotignum balticum.

PubMed

Habe, Hiroshi; Kobuna, Akinori; Hosoda, Akifumi; Kosaka, Tomoyuki; Endoh, Takayuki; Tamura, Hiroto; Yamane, Hisakazu; Nojiri, Hideaki; Omori, Toshio; Watanabe, Kazuya

2009-07-01

Desulfotignum balticum utilizes benzoate coupled to sulfate reduction. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis was conducted to detect proteins that increased more after growth on benzoate than on butyrate. A comparison of proteins on 2D gels showed that at least six proteins were expressed. The N-terminal sequences of three proteins exhibited significant identities with the alpha and beta subunits of electron transfer flavoprotein (ETF) from anaerobic aromatic-degraders. By sequence analysis of the fosmid clone insert (37,590 bp) containing the genes encoding the ETF subunits, we identified three genes, whose deduced amino acid sequences showed 58%, 74%, and 62% identity with those of Gmet_2267 (Fe-S oxidoreductase), Gmet_2266 (ETF beta subunit), and Gmet_2265 (ETF alpha subunit) respectively, which exist within the 300-kb genomic island of aromatic-degradation genes from Geobacter metallireducens GS-15. The genes encoding ETF subunits found in this study were upregulated in benzoate utilization.

The South Eastern Europe Brigade: An Option for NATO-Led Peace Support Operations?

DTIC Science & Technology

2011-06-10

Exercise CSSB Combat Service Support Battalion DOS Day of Supply DRO Disaster Relief Operations EOD Explosive Ordnance Disposal ETF Engineer Task...the adoption of the second Additional Protocol, the SEEBRIG member nations established an Engineer Task Force ( ETF ). The concept embeds purpose...capabilities, like the ETF structure, could be used as niche military capabilities by outside organizations that may need them. SEEBRIG engineer capabilities

Uniaxial creep property and viscoelastic-plastic modelling of ethylene tetrafluoroethylene (ETFE) foil

NASA Astrophysics Data System (ADS)

Li, Yintang; Wu, Minger

2015-02-01

Ethylene tetrafluoroethylene (ETFE) foil has been widely used in spatial structures for its light weight and high transparency. This paper studies short- and long-term creep properties of ETFE foil. Two series of short-term creep and recovery tests were performed, in which residual strain was observed. A long-term creep test of ETFE foil was also conducted and lasted about 400 days. A viscoelastic-plastic model was then established to describe short-term creep and recovery behaviour of ETFE foil. This model contains a traditional generalised Kelvin part and an added steady-flow component to represent viscoelastic and viscoplastic behaviour, respectively. The model can fit tests' data well at three stresses and six temperatures. Additionally, time-temperature superposition was adopted to simulate long-term creep behaviour of ETFE foil. Horizontal shifting factors were determined by W.L.F. equation in which transition temperature was simulated by shifting factors. Using this equation, long-term creep behaviours at three temperatures were predicted. The results of the long-term creep test showed that a short-term creep test at identical temperatures was insufficient to predict additional creep behaviour, and the long-term creep test verified horizontal shifting factors which were derived from the time-temperature superposition.

Post-irradiation time effects on the graft of poly(ethylene-alt-tetrafluoroethylene) (ETFE) films for ion exchange membrane application

NASA Astrophysics Data System (ADS)

Geraldes, Adriana N.; Zen, Heloísa A.; Ribeiro, Geise; Ferreira, Henrique P.; Souza, Camila P.; Parra, Duclerc F.; Santiago, Elisabete I.; Lugão, Ademar B.

2010-03-01

Grafting of styrene followed by sulfonation onto poly(ethylene-alt-tetrafluoroethylene) (ETFE) was studied for synthesis of ion exchange membranes. Radiation-induced grafting of styrene onto ETFE films was investigated after simultaneous irradiation (in post-irradiation condition) using a 60Co source. The ETFE films were irradiated at 20 kGy dose at room temperature and chemical changes were monitored after contact with styrene for grafting. The post-irradiation time was established at 14 days when the films were remained in styrene/toluene 1:1 v/v. After this period the grafting degree was evaluated in the samples. The grafted films were sulfonated using chlorosulfonic acid and 1, 2-dichloroethane 20:80 (v/v) at room temperature for 5 h. The membranes were analyzed by infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), thermogravimetric measurements (TG) and degree of grafting (DOG). The ion exchange capacity (IEC) of membranes was determined by acid-base titration and the values for ETFE membranes were achieved higher than Nafion ® films. Preliminary single cell performance was made using pure H 2 and O 2 as reactants at a cell temperature of 80 °C and atmospheric gas pressure. The fuel cell performance of ETFE films was satisfactory when compared to state-of-art Nafion ® membranes.

cDNA cloning and characterization of mouse DTEF-1 and ETF, members of the TEA/ATTS family of transcription factors.

PubMed

Yockey, C E; Shimizu, N

1998-02-01

Members of the TEA/ATTS family of transcription factors have been found in most representative eukaryotic organisms. In vertebrates, the TEA family contains at least four members, which share overlapping DNA-binding specificity and have similar transcriptional activation properties. In this article, we describe the cDNA cloning and characterization of the murine TEA proteins DTEF-1 (mDTEF-1) and ETF. Using in situ hybridization analysis of mouse embryos, we found that mDTEF-1 and ETF transcript distributions substantially overlap. ETF is expressed throughout the embryo except in the myocardium early in development, whereas late in development, it is enriched in lung and neuroectoderm. Mouse DTEF-1 is expressed at a much lower level throughout development and is substantially enriched in ectoderm and skin, as well as in the developing pituitary at midgestation. Northern blot analysis of adult mouse tissue total RNA showed that both ETF and mDTEF-1 are abundant in uterus and lung relative to other tissues. Using gel mobility shift assays and GAL4-fusion protein analysis, we demonstrated that the full coding sequences of ETF and mDTEF-1 encode M-CAT/GT-IIC-binding proteins containing activation domains.

Functional characterization of electron-transferring flavoprotein and its dehydrogenase required for fungal development and plant infection by the rice blast fungus

PubMed Central

Li, Ya; Zhu, Jindong; Hu, Jiexiong; Meng, Xiuli; Zhang, Qi; Zhu, Kunpeng; Chen, Xiaomin; Chen, Xuehang; Li, Guangpu; Wang, Zonghua; Lu, Guodong

2016-01-01

Electron-transferring flavoprotein (ETF) and its dehydrogenase (ETFDH) are highly conserved electron carriers which mainly function in mitochondrial fatty acid β oxidation. Here, we report the identification and characterization of ETF α and β subunit encoding genes (ETFA and ETFB) and ETFDH encoding gene (ETFDH) in the rice blast fungus Magnaporthe oryzae. It was demonstrated that, by impacting fatty acid metabolism, ETF and ETFDH mutations led to severe growth and conidiation defects, which could be largely rescued by exogenous acetate or carbonate. Furthermore, although conidium germination and appressorium formation appeared to be normal in ETF and ETFDH mutants, most appressoria failed to penetrate the host epidermis due to low turgor pressure. The few appressoria that succeeded in penetration were severely restricted in invasive growth and consequently failed to cause disease. Moreover, ETF mutant etfb− induced ROS accumulation in infected host cells and exogenous antioxidant GSH accelerated mutant invading growth without increasing the penetration rate. In addition, mutant etfb− displayed elevated lipid body accumulation and reduced ATP synthesis. Taken together, ETF and ETFDH play an important role in fungal development and plant infection in M. oryzae by regulation of fatty acid metabolism, turgor establishment and induction of host ROS accumulation. PMID:27113712

Functional characterization of electron-transferring flavoprotein and its dehydrogenase required for fungal development and plant infection by the rice blast fungus.

PubMed

Li, Ya; Zhu, Jindong; Hu, Jiexiong; Meng, Xiuli; Zhang, Qi; Zhu, Kunpeng; Chen, Xiaomin; Chen, Xuehang; Li, Guangpu; Wang, Zonghua; Lu, Guodong

2016-04-26

Electron-transferring flavoprotein (ETF) and its dehydrogenase (ETFDH) are highly conserved electron carriers which mainly function in mitochondrial fatty acid β oxidation. Here, we report the identification and characterization of ETF α and β subunit encoding genes (ETFA and ETFB) and ETFDH encoding gene (ETFDH) in the rice blast fungus Magnaporthe oryzae. It was demonstrated that, by impacting fatty acid metabolism, ETF and ETFDH mutations led to severe growth and conidiation defects, which could be largely rescued by exogenous acetate or carbonate. Furthermore, although conidium germination and appressorium formation appeared to be normal in ETF and ETFDH mutants, most appressoria failed to penetrate the host epidermis due to low turgor pressure. The few appressoria that succeeded in penetration were severely restricted in invasive growth and consequently failed to cause disease. Moreover, ETF mutant etfb(-) induced ROS accumulation in infected host cells and exogenous antioxidant GSH accelerated mutant invading growth without increasing the penetration rate. In addition, mutant etfb(-) displayed elevated lipid body accumulation and reduced ATP synthesis. Taken together, ETF and ETFDH play an important role in fungal development and plant infection in M. oryzae by regulation of fatty acid metabolism, turgor establishment and induction of host ROS accumulation.

The electron transfer flavoprotein: ubiquinone oxidoreductases.

PubMed

Watmough, Nicholas J; Frerman, Frank E

2010-12-01

Electron transfer flavoprotein: ubiqionone oxidoreductase (ETF-QO) is a component of the mitochondrial respiratory chain that together with electron transfer flavoprotein (ETF) forms a short pathway that transfers electrons from 11 different mitochondrial flavoprotein dehydrogenases to the ubiquinone pool. The X-ray structure of the pig liver enzyme has been solved in the presence and absence of a bound ubiquinone. This structure reveals ETF-QO to be a monotopic membrane protein with the cofactors, FAD and a [4Fe-4S](+1+2) cluster, organised to suggests that it is the flavin that serves as the immediate reductant of ubiquinone. ETF-QO is very highly conserved in evolution and the recombinant enzyme from the bacterium Rhodobacter sphaeroides has allowed the mutational analysis of a number of residues that the structure suggested are involved in modulating the reduction potential of the cofactors. These experiments, together with the spectroscopic measurement of the distances between the cofactors in solution have confirmed the intramolecular pathway of electron transfer from ETF to ubiquinone. This approach can be extended as the R. sphaeroides ETF-QO provides a template for investigating the mechanistic consequences of single amino acid substitutions of conserved residues that are associated with a mild and late onset variant of the metabolic disease multiple acyl-CoA dehydrogenase deficiency (MADD). Copyright © 2010 Elsevier B.V. All rights reserved.

Assessment of eustachian tube function in tympanoplasty.

PubMed

Prasad, Kishore Chandra; Hegde, Mahesh Chandra; Prasad, Sampath Chandra; Meyappan, Hari

2009-06-01

A clinically useful test for eustachian tube function (ETF) is still lacking. Here we plan to evaluate the mucociliary function of the ET by saccharin and methylene blue test, and compare the outcome of surgery with normal and abnormal ET functions. Case series with planned data collection. Department of Otolaryngology-Head and Neck Surgery, Kasturba Medical College, Mangalore (Manipal University), a tertiary care center in South India. This study comprised 86 patients diagnosed with mucosal chronic otitis media in quiescent/inactive stage. All were subjected to a detailed clinical examination and investigations. Preoperative evaluation of ETF was compared with postoperative outcome of surgery, and the results were analyzed. The saccharin test and methylene blue dye test had a good correlation in evaluating ETF. The mean value for saccharin perception time and the clearance time for methylene blue were 17.5 and 8.1 minutes, respectively. ETF was best in anterior, worst among posterior, and intermediate in subtotal perforations. Type 1 tympanoplasty was successful in 94 percent with normal ETF and in 68 percent with partial dysfunction. The saccharin test is a simple, cost-effective, and valuable diagnostic tool to assess the mucociliary function of the ET. The outcome of middle ear surgery would be a success in normal ETF, whereas in partial dysfunction the outcome need not necessarily be a failure.

Autologous Transplantation in Follicular Lymphoma with Early Therapy Failure: A National LymphoCare Study and Center for International Blood and Marrow Transplant Research Analysis.

PubMed

Casulo, Carla; Friedberg, Jonathan W; Ahn, Kwang W; Flowers, Christopher; DiGilio, Alyssa; Smith, Sonali M; Ahmed, Sairah; Inwards, David; Aljurf, Mahmoud; Chen, Andy I; Choe, Hannah; Cohen, Jonathon; Copelan, Edward; Farooq, Umar; Fenske, Timothy S; Freytes, Cesar; Gaballa, Sameh; Ganguly, Siddhartha; Jethava, Yogesh; Kamble, Rammurti T; Kenkre, Vaishalee P; Lazarus, Hillard; Lazaryan, Aleksandr; Olsson, Richard F; Rezvani, Andrew R; Rizzieri, David; Seo, Sachiko; Shah, Gunjan L; Shah, Nina; Solh, Melham; Sureda, Anna; William, Basem; Cumpston, Aaron; Zelenetz, Andrew D; Link, Brian K; Hamadani, Mehdi

2018-06-01

Patients with follicular lymphoma (FL) experiencing early therapy failure (ETF) within 2 years of frontline chemoimmunotherapy have poor overall survival (OS). We analyzed data from the Center for International Blood and Marrow Transplant Research (CIBMTR) and the National LymphoCare Study (NLCS) to determine whether autologous hematopoietic cell transplant (autoHCT) can improve outcomes in this high-risk FL subgroup. ETF was defined as failure to achieve at least partial response after frontline chemoimmunotherapy or lymphoma progression within 2 years of frontline chemoimmunotherapy. We identified 2 groups: the non-autoHCT cohort (patients from the NLCS with ETF not undergoing autoHCT) and the autoHCT cohort (CIBMTR patients with ETF undergoing autoHCT). All patients received rituximab-based chemotherapy as frontline treatment; 174 non-autoHCT patients and 175 autoHCT patients were identified and analyzed. There was no difference in 5-year OS between the 2 groups (60% versus 67%, respectively; P = .16). A planned subgroup analysis showed that patients with ETF receiving autoHCT soon after treatment failure (≤1 year of ETF; n = 123) had higher 5-year OS than those without autoHCT (73% versus 60%, P = .05). On multivariate analysis, early use of autoHCT was associated with significantly reduced mortality (hazard ratio, .63; 95% confidence interval, .42 to .94; P = .02). Patients with FL experiencing ETF after frontline chemoimmunotherapy lack optimal therapy. We demonstrate improved OS when receiving autoHCT within 1 year of treatment failure. Results from this unique collaboration between the NLCS and CIBMTR support consideration of early consolidation with autoHCT in select FL patients experiencing ETF. Copyright © 2017 The American Society for Blood and Marrow Transplantation. Published by Elsevier Inc. All rights reserved.

The iron-sulfur cluster of electron transfer flavoprotein-ubiquinone oxidoreductase is the electron acceptor for electron transfer flavoprotein.

PubMed

Swanson, Michael A; Usselman, Robert J; Frerman, Frank E; Eaton, Gareth R; Eaton, Sandra S

2008-08-26

Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) accepts electrons from electron transfer flavoprotein (ETF) and reduces ubiquinone from the ubiquinone pool. It contains one [4Fe-4S] (2+,1+) and one FAD, which are diamagnetic in the isolated oxidized enzyme and can be reduced to paramagnetic forms by enzymatic donors or dithionite. In the porcine protein, threonine 367 is hydrogen bonded to N1 and O2 of the flavin ring of the FAD. The analogous site in Rhodobacter sphaeroides ETF-QO is asparagine 338. Mutations N338T and N338A were introduced into the R. sphaeroides protein by site-directed mutagenesis to determine the impact of hydrogen bonding at this site on redox potentials and activity. The mutations did not alter the optical spectra, EPR g-values, spin-lattice relaxation rates, or the [4Fe-4S] (2+,1+) to FAD point-dipole interspin distances. The mutations had no impact on the reduction potential for the iron-sulfur cluster, which was monitored by changes in the continuous wave EPR signals of the [4Fe-4S] (+) at 15 K. For the FAD semiquinone, significantly different potentials were obtained by monitoring the titration at 100 or 293 K. Based on spectra at 293 K the N338T mutation shifted the first and second midpoint potentials for the FAD from +47 and -30 mV for wild type to -11 and -19 mV, respectively. The N338A mutation decreased the potentials to -37 and -49 mV. Lowering the midpoint potentials resulted in a decrease in the quinone reductase activity and negligible impact on disproportionation of ETF 1e (-) catalyzed by ETF-QO. These observations indicate that the FAD is involved in electron transfer to ubiquinone but not in electron transfer from ETF to ETF-QO. Therefore, the iron-sulfur cluster is the immediate acceptor from ETF.

Kinetic and spectral properties of isovaleryl-CoA dehydrogenase and interaction with ligands.

PubMed

Mohsen, Al-Walid A; Vockley, Jerry

2015-01-01

Isovaleryl-CoA dehydrogenase (IVD) catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA and the transfer of electrons to the electron transfer flavoprotein (ETF). Recombinant human IVD purifies with bound CoA-persulfide. A modified purification protocol was developed to isolate IVD without bound CoA-persulfide and to protect the protein thiols from oxidation. The CoA-persulfide-free IVD specific activity was 112.5 μmol porcine ETF min(-)(1) mg(-)(1), which was ∼20-fold higher than that of its CoA-persulfide bound form. The Km and catalytic efficiency (kcat/Km) for isovaleryl-CoA were 1.0 μM and 4.3 × 10(6) M(-1) s(-1) per monomer, respectively, and its Km for ETF was 2.0 μM. Anaerobic titration of isovaleryl-CoA into an IVD solution resulted in a stable blue complex with increased absorbance at 310 nm, decreased absorbance at 373 and 447 nm, and the appearance of the charge transfer complex band at 584 nm. The apparent dissociation constant (KDapp) determined spectrally for isovaleryl-CoA was 0.54 μM. Isovaleryl-CoA, acetoacetyl-CoA, methylenecyclopropyl-acetyl-CoA, and ETF induced CD spectral changes at the 250-500 nm region while isobutyryl-CoA did not, suggesting conformational changes occur at the flavin ring that are ligand specific. Replacement of the IVD Trp166 with a Phe did not block IVD interaction with ETF, indicating that its indole ring is not essential for electron transfer to ETF. A twelve amino acid synthetic peptide that matches the sequence of the ETF docking peptide competitively inhibited the enzyme reaction when ETF was used as the electron acceptor with a Ki of 1.5 mM. Copyright © 2014 Elsevier B.V. and Société française de biochimie et biologie Moléculaire (SFBBM). All rights reserved.

The Iron-Sulfur Cluster of Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase Is the Electron Acceptor for Electron Transfer Flavoprotein†

PubMed Central

Swanson, Michael A.; Usselman, Robert J.; Frerman, Frank E.; Eaton, Gareth R.; Eaton, Sandra S.

2009-01-01

Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) accepts electrons from electron transfer flavoprotein (ETF) and reduces ubiquinone from the ubiquinone pool. It contains one [4Fe-4S]2+,1+ and one FAD, which are diamagnetic in the isolated oxidized enzyme and can be reduced to paramagnetic forms by enzymatic donors or dithionite. In the porcine protein, threonine 367 is hydrogen bonded to N1 and O2 of the flavin ring of the FAD. The analogous site in Rhodobacter sphaeroides ETF-QO is asparagine 338. Mutations N338T and N338A were introduced into the R. sphaeroides protein by site-directed mutagenesis to determine the impact of hydrogen bonding at this site on redox potentials and activity. The mutations did not alter the optical spectra, EPR g-values, spin-lattice relaxation rates, or the [4Fe-4S]2+,1+ to FAD point-dipole interspin distances. The mutations had no impact on the reduction potential for the iron-sulfur cluster, which was monitored by changes in the continuous wave EPR signals of the [4Fe-4S]+ at 15 K. For the FAD semiquinone, significantly different potentials were obtained by monitoring the titration at 100 or 293 K. Based on spectra at 293 K the N338T mutation shifted the first and second midpoint potentials for the FAD from +47 and -30 mV for wild type to -11 and -19 mV, respectively. The N338A mutation decreased the potentials to -37 and -49 mV. Lowering the midpoint potentials resulted in a decrease in the quinone reductase activity and negligible impact on disproportionation of ETF1e- catalyzed by ETF-QO. These observations indicate that the FAD is involved in electron transfer to ubiquinone but not in electron transfer from ETF to ETF-QO. Therefore, the iron-sulfur cluster is the immediate acceptor from ETF. PMID:9585549

Magnetohydrodynamics (MHD) Engineering Test Facility (ETF) 200 MWe power plant. Conceptual Design Engineering Report (CDER). Volume 2: Engineering. Volume 3: Costs and schedules

NASA Astrophysics Data System (ADS)

1981-09-01

Engineering design details for the principal systems, system operating modes, site facilities, and structures of an engineering test facility (ETF) of a 200 MWE power plant are presented. The ETF resembles a coal-fired steam power plant in many ways. It is analogous to a conventional plant which has had the coal combustor replaced with the MHD power train. Most of the ETF components are conventional. They can, however, be sized or configured differently or perform additional functions from those in a conventional coal power plant. The boiler not only generates steam, but also performs the functions of heating the MHD oxidant, recovering seed, and controlling emissions.

Magnetohydrodynamics (MHD) Engineering Test Facility (ETF) 200 MWe power plant. Conceptual Design Engineering Report (CDER). Volume 2: Engineering. Volume 3: Costs and schedules

NASA Technical Reports Server (NTRS)

1981-01-01

Engineering design details for the principal systems, system operating modes, site facilities, and structures of an engineering test facility (ETF) of a 200 MWE power plant are presented. The ETF resembles a coal-fired steam power plant in many ways. It is analogous to a conventional plant which has had the coal combustor replaced with the MHD power train. Most of the ETF components are conventional. They can, however, be sized or configured differently or perform additional functions from those in a conventional coal power plant. The boiler not only generates steam, but also performs the functions of heating the MHD oxidant, recovering seed, and controlling emissions.

Physics Goals for the Planned Next Linear Collider Engineering Test Facility

NASA Astrophysics Data System (ADS)

Raubenheimer, T. O.

2001-10-01

The Next Linear Collider (NLC) Collaboration is planning to construct an Engineering Test Facility (ETF) at Fermilab. As presently envisioned, the ETF would comprise a fundamental unit of the NLC main linac to include X-band klystrons and modulators, a delay-line power-distribution system (DLDS), and NLC accelerating structures that serve as loads. The principal purpose of the ETF is to validate stable operation of the power-distribution system, first without beam, then with a beam having the NLC pulse structure. This paper concerns the possibility of configuring and using the ETF to accelerate beam with an NLC pulse structure, as well as of doing experiments to measure beam-induced wakefields in the rf structures and their influence back on the beam.

Design and construction of a time-of-flight wall detector at External Target Facility of HIRFL-CSR

NASA Astrophysics Data System (ADS)

Sun, Y.; Sun, Z. Y.; Yu, Y. H.; Yan, D.; Tang, S. W.; Sun, Y. Z.; Wang, S. T.; Zhang, X. H.; Yue, K.; Fang, F.; Chen, J. L.; Zhang, Y. J.; Hu, B. T.

2018-06-01

A Time-Of-Flight Wall (TOFW) detector has been designed and constructed at the External Target Facility (ETF) of HIRFL-CSR. The detector covers a sensitive area of 1.2 × 1.2 m2 and consists of 30 modules. Each module is composed of a long plastic scintillator bar with two photo-multiplier tubes coupled at both ends for readout. The design and manufacture details are described and the test results are reported. The performance of the TOFW detector has been tested and measured with cosmic rays and a 310 MeV/u 40Ar beam. The results show that the time resolutions of all the TOFW modules are better than 128 ps, satisfying the requirements of the experiments which will be carried out at the ETF.

Kinetic and Spectral Properties of Isovaleryl-CoA Dehydrogenase and Interaction with Ligands

PubMed Central

Mohsen, Al-Walid A.; Vockley, Jerry

2014-01-01

Isovaleryl-CoA dehydrogenase (IVD) catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA and the transfer of electrons to the electron transfer flavoprotein (ETF). Recombinant human IVD purifies with bound CoA-persulfide. A modified purification protocol was developed to isolate IVD without bound CoA-persulfide and to protect the protein thiols from oxidation. The CoA-persulfide-free IVD specific activity was 112.5 µmol porcine ETF•min−1•mg−1, which was ~20-fold higher than that of its CoA-persulfide bound form. The Km and catalytic efficiency (kcat/Km) for isovaleryl-CoA were 1.0 µM and 4.3 × 106•M−1•sec−1 per monomer, respectively, and its Km for ETF was 2.0 µM. Anaerobic titration of isovaleryl-CoA into an IVD solution resulted in a stable blue complex with increased absorbance at 310 nm, decreased absorbance at 373 and 447 nm, and the appearance of the charge transfer complex band at 584 nm. The apparent dissociation constant (KD app) determined spectrally for isovaleryl-CoA was 0.54 µM. Isovaleryl-CoA, acetoacetyl-CoA, methylenecyclopropylacetyl-CoA, and ETF induced CD spectral changes at the 250–500 nm region while isobutyryl-CoA did not, suggesting conformational changes occur at the flavin ring that are ligand specific. Replacement of the IVD Trp166 with a Phe did not block IVD interaction with ETF, indicating that its indole ring is not essential for electron transfer to ETF. A twelve amino acid synthetic peptide that matches the sequence of the ETF docking peptide competitively inhibited the enzyme reaction when ETF was used as the electron acceptor with a Ki of 1.5 mM. PMID:25450250

Reduction of ferredoxin or oxygen by flavin-based electron bifurcation in Megasphaera elsdenii.

PubMed

Chowdhury, Nilanjan P; Kahnt, Jörg; Buckel, Wolfgang

2015-08-01

Over 50 years ago, it was reported that, in the anaerobic rumen bacterium Megasphaera elsdenii, the reduction of crotonyl-CoA to butyryl-CoA by NADH involved an electron transferring flavoprotein (Etf) as mediator [Baldwin RL, Milligan LP (1964) Biochim Biophys Acta 92, 421-432]. Purification and spectroscopic characterization revealed that this Etf contained 2 FAD, whereas, in the Etfs from aerobic and facultative bacteria, one FAD is replaced by AMP. Recently we detected a similar system in the related anaerobe Acidaminococcus fermentans that differed in the requirement of additional ferredoxin as electron acceptor. The whole process was established as flavin-based electron bifurcation in which the exergonic reduction of crotonyl-CoA by NADH mediated by Etf + butyryl-CoA dehydrogenase (Bcd) was coupled to the endergonic reduction of ferredoxin also by NADH. In the present study, we demonstrate that, under anaerobic conditions, Etf + Bcd from M. elsdenii bifurcate as efficiently as Etf + Bcd from A. fermentans. Under the aerobic conditions used in the study by Baldwin and Milligan and in the presence of catalytic amounts of crotonyl-CoA or butyryl-CoA, however, Etf + Bcd act as NADH oxidase producing superoxide and H2 O2 , whereas ferredoxin is not required. We hypothesize that, during bifurcation, oxygen replaces ferredoxin to yield superoxide. In addition, the formed butyryl-CoA is re-oxidized by a second oxygen molecule to crotonyl-CoA, resulting in a stoichiometry of 2 NADH consumed and 2 H2 O2 formed. As a result of the production of reactive oxygen species, electron bifurcation can be regarded as an Achilles' heel of anaerobes when exposed to air. © 2015 FEBS.

Characterization of mitochondrial proteome in a severe case of ETF-QO deficiency.

PubMed

Rocha, H; Ferreira, R; Carvalho, J; Vitorino, R; Santa, C; Lopes, L; Gregersen, N; Vilarinho, L; Amado, F

2011-12-10

Multiple acyl-CoA dehydrogenase deficiency (MADD) is a mitochondrial fatty acid oxidation disorder caused by mutations that affect electron transfer flavoprotein (ETF) or ETF:ubiquinone oxidoreductase (ETF-QO) or even due to unidentified disturbances of riboflavin metabolism. Besides all the available data on the molecular basis of FAO disorders, including MADD, the pathophysiological mechanisms underlying clinical phenotype development, namely at the mitochondrial level, are poorly understood. In order to contribute to the elucidation of these mechanisms, we isolated mitochondria from cultured fibroblasts, from a patient with a severe MADD presentation due to ETF-QO deficiency, characterize its mitochondrial proteome and compare it with normal controls. The used approach (2-DE-MS/MS) allowed the positive identification of 287 proteins in both patient and controls, presenting 35 of the significant differences in their relative abundance. Among the differentially expressed are proteins associated to binding/folding functions, mitochondrial antioxidant enzymes as well as proteins associated to apoptotic events. The overexpression of chaperones like Hsp60 or mitochondrial Grp75, antioxidant enzymes and apoptotic proteins reflects the mitochondrial response to a complete absence of ETF-QO. Our study provides a global perspective of the mitochondrial proteome plasticity in a severe case of MADD and highlights the main molecular pathways involved in its pathogenesis. Copyright © 2011 Elsevier B.V. All rights reserved.

Diarrhoea risk factors in enterally tube fed critically ill patients: a retrospective audit.

PubMed

Jack, Leanne; Coyer, Fiona; Courtney, Mary; Venkatesh, Bala

2010-12-01

Diarrhoea in the enterally tube fed (ETF) intensive care unit (ICU) patient is a multi-factorial problem. Diarrhoeal aetiologies in this patient cohort remain debatable; however, the consequences of diarrhoea have been well established and include electrolyte imbalance, dehydration, bacterial translocation, peri anal wound contamination and sleep deprivation. This study examined the incidence of diarrhoea and explored factors contributing to the development of diarrhoea in the ETF, critically ill, adult patient. After institutional ethical review and approval, a single centre medical chart audit was undertaken to examine the incidence of diarrhoea in ETF, critically ill patients. Retrospective, non-probability sequential sampling was used of all emergency admission adult ICU patients who met the inclusion/exclusion criteria. Fifty patients were audited. Faecal frequency, consistency and quantity were considered important criteria in defining ETF diarrhoea. The incidence of diarrhoea was 78%. Total patient diarrhoea days (r=0.422; p=0.02) and total diarrhoea frequency (r=0.313; p=0.027) increased when the patient was ETF for longer periods of time. Increased severity of illness, peripheral oxygen saturation (Sp02), glucose control, albumin and white cell count were found to be statistically significant factors for the development of diarrhoea. Diarrhoea in ETF critically ill patients is multi-factorial. The early identification of diarrhoea risk factors and the development of a diarrhoea risk management algorithm is recommended. Copyright © 2010. Published by Elsevier Ltd.

Solvent effect on post-irradiation grafting of styrene onto poly(ethylene-alt-tetrafluoroethylene) (ETFE) films

NASA Astrophysics Data System (ADS)

Napoleão Geraldes, Adriana; Augusto Zen, Heloísa; Ribeiro, Geise; Fernandes Parra, Duclerc; Benévolo Lugão, Ademar

2013-03-01

Radiation-induced grafting of styrene onto ETFE films in different solvent was investigated after simultaneous irradiation (in post-irradiation condition) using a 60Co source. Grafting of styrene followed by sulfonation onto poly(ethylene-alt-tetrafluoroethylene) (ETFE) are currently studied for synthesis of ion exchange membranes. The ETFE films were immersed in styrene/toluene, styrene/methanol and styrene/isopropyl alcohol and irradiated at 20 and 100 kGy doses at room temperature. The post-irradiation time was established at 14 day and the grafting degree was evaluated. The grafted films were sulfonated using chlorosulfonic acid and 1,2-dichloroethane 20:80 (v/v) at room temperature for 5 h. The degree of grafting (DOG) was determined gravimetrically and physical or chemical changes were evaluated by differential scanning calorimeter analysis (DSC), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). The ion exchange capacity (IEC) values showed the best performance of sulfonation for ETFE membranes grafted in toluene solvent. Surface images of the grafted films by SEM technique have presented a strong effect of the solvents on the films morphology.

Effort test failure: toward a predictive model.

PubMed

Webb, James W; Batchelor, Jennifer; Meares, Susanne; Taylor, Alan; Marsh, Nigel V

2012-01-01

Predictors of effort test failure were examined in an archival sample of 555 traumatically brain-injured (TBI) adults. Logistic regression models were used to examine whether compensation-seeking, injury-related, psychological, demographic, and cultural factors predicted effort test failure (ETF). ETF was significantly associated with compensation-seeking (OR = 3.51, 95% CI [1.25, 9.79]), low education (OR:. 83 [.74, . 94]), self-reported mood disorder (OR: 5.53 [3.10, 9.85]), exaggerated displays of behavior (OR: 5.84 [2.15, 15.84]), psychotic illness (OR: 12.86 [3.21, 51.44]), being foreign-born (OR: 5.10 [2.35, 11.06]), having sustained a workplace accident (OR: 4.60 [2.40, 8.81]), and mild traumatic brain injury severity compared with very severe traumatic brain injury severity (OR: 0.37 [0.13, 0.995]). ETF was associated with a broader range of statistical predictors than has previously been identified and the relative importance of psychological and behavioral predictors of ETF was evident in the logistic regression model. Variables that might potentially extend the model of ETF are identified for future research efforts.

Physics Goals for the Planned Next Linear Collider Engineering Test Facility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Raubenheimer, Tor O

2001-10-02

The Next Linear Collider (NLC) Collaboration is planning to construct an Engineering Test Facility (ETF) at Fermilab. As presently envisioned, the ETF would comprise a fundamental unit of the NLC main linac to include X-band klystrons and modulators, a delay-line power-distribution system (DLDS), and NLC accelerating structures that serve as loads. The principal purpose of the ETF is to validate stable operation of the power-distribution system, first without beam, then with a beam having the NLC pulse structure. This paper concerns the possibility of configuring and using the ETF to accelerate beam with an NLC pulse structure, as well asmore » of doing experiments to measure beam-induced wakefields in the rf structures and their influence back on the beam.« less

Physics goals for the planned next linear collider engineering test facility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Courtlandt L Bohn et al.

2001-06-26

The Next Linear Collider (NLC) Collaboration is planning to construct an Engineering Test Facility (ETF) at Fermilab. As presently envisioned, the ETF would comprise a fundamental unit of the NLC main linac to include X-band klystrons and modulators, a delay-line power-distribution system (DLDS), and NLC accelerating structures that serve as loads. The principal purpose of the ETF is to validate stable operation of the power-distribution system, first without beam, then with a beam having the NLC pulse structure. This paper concerns the possibility of configuring and using the ETF to accelerate beam with an NLC pulse structure, as well asmore » of doing experiments to measure beam-induced wakefields in the rf structures and their influence back on the beam.« less

Physics goals for the planned next linear collider engineering test facility.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bohn, C.; Michelotti, L.; Ostiguy, J.-F.

2001-07-17

The Next Linear Collider (NLC) Collaboration is planning to construct an Engineering Test Facility (ETF) at Fermilab. As presently envisioned, the ETF would comprise a fundamental unit of the NLC main linac to include X-band klystrons and modulators, a delay-line power-distribution system (DLDS), and NLC accelerating structures that serve as loads. The principal purpose of the ETF is to validate stable operation of the power-distribution system, first without beam, then with a beam having the NLC pulse structure. This paper concerns the possibility of configuring and using the ETF to accelerate beam with an NLC pulse structure, as well asmore » of doing experiments to measure beam-induced wakefields in the rf structures and their influence back on the beam.« less

SECONDARY WASTE MANAGEMENT FOR HANFORD EARLY LOW ACTIVITY WASTE VITRIFICATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

UNTERREINER BJ

2008-07-18

More than 200 million liters (53 million gallons) of highly radioactive and hazardous waste is stored at the U.S. Department of Energy's Hanford Site in southeastern Washington State. The DOE's Hanford Site River Protection Project (RPP) mission includes tank waste retrieval, waste treatment, waste disposal, and tank farms closure activities. This mission will largely be accomplished by the construction and operation of three large treatment facilities at the Waste Treatment and Immobilization Plant (WTP): (1) a Pretreatment (PT) facility intended to separate the tank waste into High Level Waste (HLW) and Low Activity Waste (LAW); (2) a HLW vitrification facilitymore » intended to immobilize the HLW for disposal at a geologic repository in Yucca Mountain; and (3) a LAW vitrification facility intended to immobilize the LAW for shallow land burial at Hanford's Integrated Disposal Facility (IDF). The LAW facility is on target to be completed in 2014, five years prior to the completion of the rest of the WTP. In order to gain experience in the operation of the LAW vitrification facility, accelerate retrieval from single-shell tank (SST) farms, and hasten the completion of the LAW immobilization, it has been proposed to begin treatment of the low-activity waste five years before the conclusion of the WTP's construction. A challenge with this strategy is that the stream containing the LAW vitrification facility off-gas treatment condensates will not have the option of recycling back to pretreatment, and will instead be treated by the Hanford Effluent Treatment Facility (ETF). Here the off-gas condensates will be immobilized into a secondary waste form; ETF solid waste.« less

Radioactive demonstration of final mineralized waste forms for Hanford waste treatment plant secondary waste (WTP-SW) by fluidized bed steam reforming (FBSR) using the bench scale reformer platform

DOE Office of Scientific and Technical Information (OSTI.GOV)

Crawford, C.; Burket, P.; Cozzi, A.

2014-08-01

The U.S. Department of Energy’s Office of River Protection (ORP) is responsible for the retrieval, treatment, immobilization, and disposal of Hanford’s tank waste. Currently there are approximately 56 million gallons of highly radioactive mixed wastes awaiting treatment. A key aspect of the River Protection Project (RPP) cleanup mission is to construct and operate the Waste Treatment and Immobilization Plant (WTP). The WTP will separate the tank waste into high-level and low-activity waste (LAW) fractions, both of which will subsequently be vitrified. The projected throughput capacity of the WTP LAW Vitrification Facility is insufficient to complete the RPP mission in themore » time frame required by the Hanford Federal Facility Agreement and Consent Order, also known as the Tri-Party Agreement (TPA), i.e. December 31, 2047. Therefore, Supplemental Treatment is required both to meet the TPA treatment requirements as well as to more cost effectively complete the tank waste treatment mission. In addition, the WTP LAW vitrification facility off-gas condensate known as WTP Secondary Waste (WTP-SW) will be generated and enriched in volatile components such as 137Cs, 129I, 99Tc, Cl, F, and SO4 that volatilize at the vitrification temperature of 1150°C in the absence of a continuous cold cap (that could minimize volatilization). The current waste disposal path for the WTP-SW is to process it through the Effluent Treatment Facility (ETF). Fluidized Bed Steam Reforming (FBSR) is being considered for immobilization of the ETF concentrate that would be generated by processing the WTP-SW. The focus of this current report is the WTP-SW.« less

Automatic Optical Crack Tracking for Double Cantilever Beam Specimens

DTIC Science & Technology

2015-01-01

Developments Corp., Brookeville, OH, USA) are stacked in a [90/0]8 layup sequence. An Ethylene tetrafluoroethylene ( ETFE ) film (25μm thick) is placed...DCB samples are cut using a diamond-blade wet saw from the 4-mm-thick panel to approximately 25 mm wide and 150 mm long (60 mm ETFE , 90 mm neat...to the interior ETFE film termination interface is approximately 47 mm. Crosshead speed is (+) 5 mm/min during loading and (−) 25 mm/min for

Morphological Study of Poly(vinylbenzyl chloride)-Grafted Poly(ethylene-co-tetrafluoroethylene) [ETFE-g-PVBC] Films Using Small-Angle Neutron Scattering Analysis.

PubMed

Song, J M; Ko, B S; Sohn, J Y; Shin, J

2016-06-01

In this study, the effect of degree of the grafting and crosslinking on the morphology of the crystalline domain in poly(vinylbenzyl chloride)-grafted poly(ethylene-co-tetrafluoroethylene) [ETFE-g-PVBC] films was investigated using a SANS (small-angle neutron scattering) analysis. The grafted films can be used as a precursor for ion-exchange membrane. ETFE-g-PVBC films with various degrees of cross-linking were prepared by a simultaneous irradiation grafting of vinylbenzyl chloride (VBC) and divinylbenzene (DVB) onto an ETFE film. The SEM-EDX (scanning electron microscopy-energy dispersive X-ray spectroscopy) results of a cross-sectional distribution of ETFE-g-PVBC films showed that the chlorine atoms were well-distributed throughout the films. SANS profiles of the PVBC-grafted films in the absence of a DVB crosslinker showed that the crystalline domain peaks were observed and the peak maximum position shifted significantly from 0.032 Å-(-1) to 0.02 Å(-1) with an increase in the degree of grafting. However, peak maximum positions of the PVBC-grafted films in the presence of a DVB crosslinker shifted slightly from 0.02 Å(-1) to 0.024 Å(-1) with an increase in the amount of DVB monomer at same degree of grafting. These results indicate that the degree of grafting and crosslinking affect the morphology of the crystalline domain in the ETFE-g-PVBC films.

Effects of low and high energy ion bombardment on ETFE polymer

NASA Astrophysics Data System (ADS)

Minamisawa, R. A.; De Almeida, A.; Abidzina, V.; Parada, M. A.; Muntele, I.; Ila, D.

2007-04-01

The polymer ethylenetetrafluoroethylene (ETFE) is used as anti-adherent coatings for food packages and radiation dosimeters. In this work, we compare the damage induced in ETFE bombarded with 100 keV Si ions with that induced by 1 MeV proton bombardment. The damage depends on the type, energy and intensity of the irradiation. Irradiated films were analyzed with optical absorption photospectrometry, Raman spectroscopy and Fourier transform infrared (FTIR) spectroscopy to determine the chemical nature of the structural changes caused by ion irradiation. Computer simulations were performed to evaluate the radiation damage.

Development and Validation of a Stability-Indicating Assay of Etofenamate by RP-HPLC and Characterization of Degradation Products

PubMed Central

Peraman, Ramalingam; Nayakanti, Devanna; Dugga, Hari Hara Theja; Kodikonda, Sudhakara

2013-01-01

A validated stability-indicating RP-HPLC method for etofenamate (ETF) was developed by separating its degradation products on a C18 (250 mm × 4.6 mm 5 μm) Qualisil BDS column using a phosphate buffer (pH-adjusted to 6.0 with orthophosphoric acid) and methanol in the ratio of 20:80 % v/v as the mobile phase at a flow rate of 1.0 mL/min. The column effluents were monitored by a photodiode array detector set at 286 nm. The method was validated in terms of specificity, linearity, accuracy, precision, detection limit, quantification limit, and robustness. Forced degradation of etofenamate was carried out under acidic, basic, thermal, photo, and peroxide conditions and the major degradation products of acidic and basic degradation were isolated and characterized by 1H-NMR, 13C-NMR, and mass spectral studies. The mass balance of the method varied between 92–99%. PMID:24482770

Impact of Mutations on the Midpoint Potential of the [4Fe-4S]+1,+2 Cluster and on Catalytic Activity in Electron Transfer Flavoprotein-ubiquinone Oxidoreductase (ETF-QO)†

PubMed Central

Usselman, Robert J.; Fielding, Alistair J.; Frerman, Frank E.; Watmough, Nicholas J.; Eaton, Gareth R.; Eaton, Sandra S.

2011-01-01

Electron transfer flavoprotein - ubiquinone oxidoreductase (ETF-QO) is an iron-sulfur flavoprotein that accepts electrons from electron-transfer flavoprotein (ETF) and reduces ubiquinone from the Q-pool. ETF-QO contains a single [4Fe-4S]2+,1+ cluster and one equivalent of FAD, which are diamagnetic in the isolated oxidized enzyme and can be reduced to paramagnetic forms by enzymatic donors or dithionite. Mutations were introduced by site-directed mutagenesis of amino acids in the vicinity of the iron-sulfur cluster of Rhodobacter sphaeroides ETF-QO. Y501 and T525 are equivalent to Y533 and T558 in the porcine ETF-QO. In the porcine protein, these residues are within hydrogen bonding distance of the Sγ of the cysteine ligands to the iron-sulfur cluster. Y501F, T525A, and Y501F/T525A substitutions were made to determine the effects on midpoint potential, activity, and EPR spectral properties of the cluster. The integrity of the mutated proteins was confirmed by optical spectra, EPR g-values, and spin-lattice relaxation rates, and the cluster to flavin point-dipole distance was determined by relaxation enhancement. Potentiometric titrations were monitored by changes in the CW EPR signals of the cluster and semiquinone. Single mutations decreased the mid-point potentials of the iron-sulfur cluster from +37 mV for wild type to −60 mV for Y501F and T525A and to −128 mV for Y501F/T525A. Lowering the midpoint potential resulted in a decrease in steady-state ubiquinone reductase activity and in ETF semiquinone disproportionation. The decrease in activity demonstrates that reduction of the iron-sulfur cluster is required for activity. There was no detectable effect of the mutations on the flavin midpoint potentials. PMID:18069858

Impact of mutations on the midpoint potential of the [4Fe-4S]+1,+2 cluster and on catalytic activity in electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO).

PubMed

Usselman, Robert J; Fielding, Alistair J; Frerman, Frank E; Watmough, Nicholas J; Eaton, Gareth R; Eaton, Sandra S

2008-01-08

Electron-transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) is an iron-sulfur flavoprotein that accepts electrons from electron-transfer flavoprotein (ETF) and reduces ubiquinone from the Q-pool. ETF-QO contains a single [4Fe-4S]2+,1+ cluster and one equivalent of FAD, which are diamagnetic in the isolated oxidized enzyme and can be reduced to paramagnetic forms by enzymatic donors or dithionite. Mutations were introduced by site-directed mutagenesis of amino acids in the vicinity of the iron-sulfur cluster of Rhodobacter sphaeroides ETF-QO. Y501 and T525 are equivalent to Y533 and T558 in the porcine ETF-QO. In the porcine protein, these residues are within hydrogen-bonding distance of the Sgamma of the cysteine ligands to the iron-sulfur cluster. Y501F, T525A, and Y501F/T525A substitutions were made to determine the effects on midpoint potential, activity, and EPR spectral properties of the cluster. The integrity of the mutated proteins was confirmed by optical spectra, EPR g-values, and spin-lattice relaxation rates, and the cluster to flavin point-dipole distance was determined by relaxation enhancement. Potentiometric titrations were monitored by changes in the CW EPR signals of the cluster and semiquinone. Single mutations decreased the midpoint potentials of the iron-sulfur cluster from +37 mV for wild type to -60 mV for Y501F and T525A and to -128 mV for Y501F/T525A. Lowering the midpoint potential resulted in a decrease in steady-state ubiquinone reductase activity and in ETF semiquinone disproportionation. The decrease in activity demonstrates that reduction of the iron-sulfur cluster is required for activity. There was no detectable effect of the mutations on the flavin midpoint potentials.

Collision Scenarios for Assessing Crashworthiness of Passenger Rail Equipment

DOT National Transportation Integrated Search

2010-10-12

In June 2009, at the request of the Federal Railroad Administration (FRA), the Railroad Safety Advisory Committee established the Engineering Task Force (ETF). The ETF is comprised of government, railroads, suppliers, and labor organizations and thei...

Biomimetic membrane arrays on cast hydrogel supports.

PubMed

Roerdink Lander, Monique; Ibragimova, Sania; Rein, Christian; Vogel, Jörg; Stibius, Karin; Geschke, Oliver; Perry, Mark; Hélix-Nielsen, Claus

2011-06-07

Lipid bilayers are intrinsically fragile and require mechanical support in technical applications based on biomimetic membranes. Tethering the lipid bilayer membranes to solid substrates, either directly through covalent or ionic substrate-lipid links or indirectly on substrate-supported cushions, provides mechanical support but at the cost of small molecule transport through the membrane-support sandwich. To stabilize biomimetic membranes while allowing transport through a membrane-support sandwich, we have investigated the feasibility of using an ethylene tetrafluoroethylene (ETFE)/hydrogel sandwich as the support. The sandwich is realized as a perforated surface-treated ETFE film onto which a hydrogel composite support structure is cast. We report a simple method to prepare arrays of lipid bilayer membranes with low intrinsic electrical conductance on the highly permeable, self-supporting ETFE/hydrogel sandwiches. We demonstrate how the ETFE/hydrogel sandwich support promotes rapid self-thinning of lipid bilayers suitable for hosting membrane-spanning proteins.

Validating simple dynamical simulations of the unitary Fermi gas

NASA Astrophysics Data System (ADS)

Forbes, Michael McNeil; Sharma, Rishi

2014-10-01

We present a comparison between simulated dynamics of the unitary fermion gas using the superfluid local density approximation (SLDA) and a simplified bosonic model, the extended Thomas-Fermi (ETF) with a unitary equation of state. Small-amplitude fluctuations have similar dynamics in both theories for frequencies far below the pair-breaking threshold and wave vectors much smaller than the Fermi momentum. The low-frequency linear responses in both match well for surprisingly large wave vectors, even up to the Fermi momentum. For nonlinear dynamics such as vortex generation, the ETF provides a semiquantitative description of SLDA dynamics as long as the fluctuations do not have significant power near the pair-breaking threshold; otherwise the dynamics of the ETF cannot be trusted. Nonlinearities in the ETF tend to generate high-frequency fluctuations, and with no normal component to remove this energy from the superfluid, features such as vortex lattices cannot relax and crystallize as they do in the SLDA.

An initial evaluation of a proof-of-concept 128-Hz electronic tuning fork in the detection of peripheral neuropathy.

PubMed

O'Brien, Todd; Karem, Joseph

2014-03-01

Diabetic peripheral neuropathy (DPN) is an essential precursor leading to diabetic limb loss. Neurologic screening tests, including the 128-Hz tuning fork (TF), have long been used to identify and track the progression of DPN, thereby guiding the implementation of preventive strategies. Although a sensitive indicator of neuropathy, shortcomings of TF testing include the lack of standardization and quantification of clinical findings. In an attempt to overcome these limitations, a novel 128-Hz electronic TF (ETF) prototype has been developed that is capable of performing accurate timed vibration tests (TVTs). This study was designed to assess the ability of the ETF to detect sensory impairment compared with three established neurologic screening methods: the Semmes-Weinstein monofilament test, the biothesiometer, and the sharp/dull discrimination test. Fifty-five test patients were recruited from the primary author's practice and enrolled according to an approved protocol. The 10-g Semmes-Weinstein monofilament test and the sharp/dull discrimination test were administered in standard fashion to the plantar aspects of digits 1 and 5 bilaterally. The ETF and the biothesiometer (25-V setting) were applied to the dorsal aspects of the distal phalanx of the hallux and fifth metatarsal head bilaterally. The sensitivity and specificity of neuropathy detection for the ETF were 0.953 and 0.761, respectively, using conventional tests as reference standards. Performance of TVTs with the ETF detected sensory impairment compared with three conventional neurologic screening methods. Given these findings, the ETF could facilitate the use of standardized TVTs as an indicator of DPN progression.

Probiotics and diarrhoea management in enterally tube fed critically ill patients--what is the evidence?

PubMed

Jack, Leanne; Coyer, Fiona; Courtney, Mary; Venkatesh, Bala

2010-12-01

The aim of this literature review is to identify the role of probiotics in the management of enteral tube feeding (ETF) diarrhoea in critically ill patients. Diarrhoea is a common gastrointestinal problem seen in ETF patients. The incidence of diarrhoea in tube fed patients varies from 2% to 68% across all patients. Despite extensive investigation, the pathogenesis surrounding ETF diarrhoea remains unclear. Evidence to support probiotics to manage ETF diarrhoea in critically ill patients remains sparse. Literature on ETF diarrhoea and probiotics in critically ill, adult patients was reviewed from 1980 to 2010. The Cochrane Library, Pubmed, Science Direct, Medline and the Cumulative Index of Nursing and Allied Health Literature (CINAHL) electronic databases were searched using specific inclusion/exclusion criteria. Key search terms used were: enteral nutrition, diarrhoea, critical illness, probiotics, probiotic species and randomised clinical control trial (RCT). Four RCT papers were identified with two reporting full studies, one reporting a pilot RCT and one conference abstract reporting an RCT pilot study. A trend towards a reduction in diarrhoea incidence was observed in the probiotic groups. However, mortality associated with probiotic use in some severely and critically ill patients must caution the clinician against its use. Evidence to support probiotic use in the management of ETF diarrhoea in critically ill patients remains unclear. This paper argues that probiotics should not be administered to critically ill patients until further research has been conducted to examine the causal relationship between probiotics and mortality, irrespective of the patient's disease state or projected prophylactic benefit of probiotic administration. Copyright © 2010 Elsevier Ltd. All rights reserved.

Thermal oxidative degradation of ethylene tetrafluoroethylene copolymer systems

NASA Astrophysics Data System (ADS)

Elders, Jonathan Patrick

Thermo-oxidative degradation of ethylene tetrafluoroethylene (ETFE) was investigated to determine how modifications for use in an electrical wire system affected its thermal stability. Modifications included electron irradiation and subsequent cross-linking during manufacture and contact with a metal surface. Samples with irradiation histories between 0 and 48 MRads were investigated. Degradation of ETFE was enhanced by contact with a metal "conductor" surface: silver - coated copper. Polymer degradation was analyzed by weight loss kinetics (thermogravimetric analysis (TGA)), changes in polymer morphology (differential scanning calorimetry (DSC)), optical microscopy, attenuated total reflectance (ATR) infrared spectroscopy, and gas chromatography - mass spectroscopy (GC/MS). Conductor aging (copper permeation through silver with subsequent oxidation) was investigated using scanning Auger Electron Spectroscopy (AES). Conductor aging is enhanced in the presence of the polymer surface. Interactions between conductor and polymer were analyzed by optical microscopy, scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). The rate of polymer degradation from 220°C to 280°C was independent of time and extent of degradation, and rate was proportional to irradiation dose. The activation energy for degradation of unirradiated ETFE was 227 kJ/mol and decreased from 150 to 138 kJ/mol for ETFE irradiated to doses between 6 and 48 MRads. Rates of degradation at 300°C to 320°C were dependent on the extent of degradation. Rates of degradation at temperatures between 230°C and 310°C were an order of magnitude larger in the presence of a conductor than in its absence, and activation energies for degradation in the presence of conductor were reduced to 120 kJ/mol. Degradation was modeled as the combination of bulk polymer degradation and catalytic degradation at the polymer-metal interface. ETFE aged at 250°C in the presence or absence of a conductor exhibited a double melting endotherm. ATR spectra of aged ETFE indicated polymer oxidation. Based on AES experiments, copper permeation during aging in the presence or absence of ETFE was consistent with Fickian diffusion. The coefficient for copper diffusion through silver was approximately 10 -15 cm2/second, and catalytic ETFE degradation was proportional to conductor aging. The copper oxidized on the surface to yield a material with a stoichiometric composition of Cu3O 2.

Electron beam irradiation effects on ethylene-tetrafluoroethylene copolymer films

NASA Astrophysics Data System (ADS)

Nasef, Mohamed Mahmoud; Saidi, Hamdani; Dahlan, Khairul Zaman M.

2003-12-01

The effects of electron beam irradiation on ethylene-tetrafluoroethylene copolymer (ETFE) films were studied. Samples were irradiated in air at room temperature by a universal electron beam accelerator for doses ranging from 100 to 1200 kGy. Irradiated samples were investigated with respect to their chemical structure, thermal characteristics, crystallinity and mechanical properties using FTIR, differential scanning calorimeter (DSC) and universal mechanical tester. The interaction of electron irradiation with ETFE films was found to induce dose-dependent changes in all the investigated properties. A mechanism for electron-induced reactions is proposed to explain the structure-property behaviour of irradiated ETFE films.

Preliminary summary of the ETF conceptual studies

NASA Technical Reports Server (NTRS)

Seikel, G. R.; Bercaw, R. W.; Pearson, C. V.; Owens, W. R.

1978-01-01

Power plant studies have shown the attractiveness of MHD topped steam power plants for baseload utility applications. To realize these advantages, a three-phase development program was initiated. In the first phase, the engineering data and experience were developed for the design and construction of a pilot plant, the Engineering Test Facility (ETF). Results of the ETF studies are reviewed. These three parallel independent studies were conducted by industrial teams led by the AVCO Everett Research Laboratory, the General Electric Corporation, and the Westinghouse Corporation. A preliminary analysis and the status of the critical evaluation of these results are presented.

Comparison of sodium naphthenate and air-ionization corona discharge as surface treatments for the ethylene-tetrafluoroethylene polymer (ETFE) to improve adhesion between ETFE and acrylonitrile-butadiene-styrene polymer (ABS) in the presence of a cyanoacrylate adhesive (CAA)

NASA Astrophysics Data System (ADS)

Lucía Johanning-Solís, Ana; Stradi-Granados, Benito A.

2014-09-01

This study compares two ethylene-tetrafluoroethylene (ETFE) surface activation treatments, namely chemical attack with a solution of sodium naphthenate and plasma erosion via air-ionization corona discharge in order to improve the adhesive properties of the ETFE. An experimental design was prepared for both treatments in order to assess the effect of the treatment characteristics on the tensile load needed to break the bond between the ETFE and the acrylonitrile-butadiene-styrene polymer (ABS) formed with a cyanoacrylate adhesive (CAA) applied between them. The reason for the selection of this problem is that both polymers are frequently used in the biomedical industry for their properties, and they need to be joined firmly in biomedical devices, and the cyanoacrylate adhesive is the adhesive traditionally used for fluoropolymers, in this case the ETFE, and the same CAA has also shown good adhesion with ABS. However, the strength of the bond for the triplet ETFE-CAA-ABS has not been reported and the improvement of the strength of the bond with surface treatments is not found in scholarly journals for modern medical devices such as stents and snares. Both treatments were compared based on the aforementioned design of experiments. The case where ETFE receives no surface treatment serves as the reference. The results indicated that the three factors evaluated (initial drying of the material, temperature of the chemical bath, and immersion time), and their interactions have no significant effect over the tensile load at failure (tensile strength) of the adhesive bond being evaluated. For the air-ionization corona discharge treatment, two factors were evaluated: discharge exposition time and air pressure. The results obtained from this experimental design indicate that there is no significant difference between the levels of the factors evaluated. These results were unexpected as the ranges used were representative of the maximum ranges permissible in manufacturing operations. As for the comparison of the treatments, it was determined that the treatments have statistically significant differences. It was also determined that there is a significant statistical difference between the processes where a surface treatment is performed and the process where no surface treatment is applied to the ETFE. The chemical treatment results in a higher tensile load at failure (tensile strength) of 276.6 N on average, the air ionization treatment has an average of 248.4 N, and the process with no treatment has the lower ultimate tensile strength average of 53 N. This comparison has demonstrated that the best treatment is the chemical treatment with sodium naphthenate under the conditions tested.

Study of the Impact of the Global Positioning System on Army Survey

DTIC Science & Technology

1976-09-01

REPRO) Sub~y~tem - To reproduce the outputs in the volume required by the user. It will also reprint stocked products which become depleted, The...line of sight. Ab;. etf Tcpot( Ch a,:c ZC - More specialized hand level in that it provides a meais of dire:tly reading the precent of slope in an area

76 FR 61956 - Electronic Tariff Filing System (ETFS)

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-06

...] Electronic Tariff Filing System (ETFS) AGENCY: Federal Communications Commission. ACTION: Final rule; announcement of effective date. SUMMARY: In this document, the Commission announces that the Office of Management and Budget (OMB) has approved, for a period of three years, the information collection associated...

75 FR 22169 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Withdrawal of Proposed Rule Change...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-27

... of AdvisorShares WCM/BNY Mellon Focused Growth ADR ETF April 21, 2010. On February 23, 2010, NYSE... Focused Growth ADR ETF (the ``Fund'') under NYSE Arca Equities Rule 8.600 (Managed Fund Shares). The...

Hybrid Evidence Theory-based Finite Element/Statistical Energy Analysis method for mid-frequency analysis of built-up systems with epistemic uncertainties

NASA Astrophysics Data System (ADS)

Yin, Shengwen; Yu, Dejie; Yin, Hui; Lü, Hui; Xia, Baizhan

2017-09-01

Considering the epistemic uncertainties within the hybrid Finite Element/Statistical Energy Analysis (FE/SEA) model when it is used for the response analysis of built-up systems in the mid-frequency range, the hybrid Evidence Theory-based Finite Element/Statistical Energy Analysis (ETFE/SEA) model is established by introducing the evidence theory. Based on the hybrid ETFE/SEA model and the sub-interval perturbation technique, the hybrid Sub-interval Perturbation and Evidence Theory-based Finite Element/Statistical Energy Analysis (SIP-ETFE/SEA) approach is proposed. In the hybrid ETFE/SEA model, the uncertainty in the SEA subsystem is modeled by a non-parametric ensemble, while the uncertainty in the FE subsystem is described by the focal element and basic probability assignment (BPA), and dealt with evidence theory. Within the hybrid SIP-ETFE/SEA approach, the mid-frequency response of interest, such as the ensemble average of the energy response and the cross-spectrum response, is calculated analytically by using the conventional hybrid FE/SEA method. Inspired by the probability theory, the intervals of the mean value, variance and cumulative distribution are used to describe the distribution characteristics of mid-frequency responses of built-up systems with epistemic uncertainties. In order to alleviate the computational burdens for the extreme value analysis, the sub-interval perturbation technique based on the first-order Taylor series expansion is used in ETFE/SEA model to acquire the lower and upper bounds of the mid-frequency responses over each focal element. Three numerical examples are given to illustrate the feasibility and effectiveness of the proposed method.

Human METTL20 Methylates Lysine Residues Adjacent to the Recognition Loop of the Electron Transfer Flavoprotein in Mitochondria*

PubMed Central

Rhein, Virginie F.; Carroll, Joe; He, Jiuya; Ding, Shujing; Fearnley, Ian M.; Walker, John E.

2014-01-01

In mammalian mitochondria, protein methylation is a relatively uncommon post-transcriptional modification, and the extent of the mitochondrial protein methylome, the modifying methyltransferases, and their substrates have been little studied. As shown here, the β-subunit of the electron transfer flavoprotein (ETF) is one such methylated protein. The ETF is a heterodimer of α- and β-subunits. Lysine residues 199 and 202 of mature ETFβ are almost completely trimethylated in bovine heart mitochondria, whereas ETFα is not methylated. The enzyme responsible for the modifications was identified as methyltransferase-like protein 20 (METTL20). In human 143B cells, the methylation of ETFβ is less extensive and is diminished further by suppression of METTL20. Tagged METTL20 expressed in HEK293T cells specifically associates with the ETF and promotes the trimethylation of ETFβ lysine residues 199 and 202. ETF serves as a mobile electron carrier linking dehydrogenases involved in fatty acid oxidation and one-carbon metabolism to the membrane-associated ubiquinone pool. The methylated residues in ETFβ are immediately adjacent to a protein loop that recognizes and binds to the dehydrogenases. Suppression of trimethylation of ETFβ in mouse C2C12 cells oxidizing palmitate as an energy source reduced the consumption of oxygen by the cells. These experiments suggest that the oxidation of fatty acids in mitochondria and the passage of electrons via the ETF may be controlled by modulating the protein-protein interactions between the reduced dehydrogenases and the β-subunit of the ETF by trimethylation of lysine residues. METTL20 is the first lysine methyltransferase to be found to be associated with mitochondria. PMID:25023281

Secondary coenzyme Q10 deficiency and oxidative stress in cultured fibroblasts from patients with riboflavin responsive multiple Acyl-CoA dehydrogenation deficiency.

PubMed

Cornelius, Nanna; Byron, Colleen; Hargreaves, Iain; Guerra, Paula Fernandez; Furdek, Andrea K; Land, John; Radford, Weston W; Frerman, Frank; Corydon, Thomas J; Gregersen, Niels; Olsen, Rikke K J

2013-10-01

Coenzyme Q10 (CoQ10) is essential for the energy production of the cells and as an electron transporter in the mitochondrial respiratory chain. CoQ10 links the mitochondrial fatty acid β-oxidation to the respiratory chain by accepting electrons from electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO). Recently, it was shown that a group of patients with the riboflavin responsive form of multiple acyl-CoA dehydrogenation deficiency (RR-MADD) carrying inherited amino acid variations in ETF-QO also had secondary CoQ10 deficiency with beneficial effects of CoQ10 treatment, thus adding RR-MADD to an increasing number of diseases involving secondary CoQ10 deficiency. In this study, we show that moderately decreased CoQ10 levels in fibroblasts from six unrelated RR-MADD patients were associated with increased levels of mitochondrial reactive oxygen species (ROS). Treatment with CoQ10, but not with riboflavin, could normalize the CoQ10 level and decrease the level of ROS in the patient cells. Additionally, riboflavin-depleted control fibroblasts showed moderate CoQ10 deficiency, but not increased mitochondrial ROS, indicating that variant ETF-QO proteins and not CoQ10 deficiency are the causes of mitochondrial ROS production in the patient cells. Accordingly, the corresponding variant Rhodobacter sphaeroides ETF-QO proteins, when overexpressed in vitro, bind a CoQ10 pseudosubstrate, Q10Br, less tightly than the wild-type ETF-QO protein, suggesting that molecular oxygen can get access to the electrons in the misfolded ETF-QO protein, thereby generating superoxide and oxidative stress, which can be reversed by CoQ10 treatment.

Human METTL20 methylates lysine residues adjacent to the recognition loop of the electron transfer flavoprotein in mitochondria.

PubMed

Rhein, Virginie F; Carroll, Joe; He, Jiuya; Ding, Shujing; Fearnley, Ian M; Walker, John E

2014-08-29

In mammalian mitochondria, protein methylation is a relatively uncommon post-transcriptional modification, and the extent of the mitochondrial protein methylome, the modifying methyltransferases, and their substrates have been little studied. As shown here, the β-subunit of the electron transfer flavoprotein (ETF) is one such methylated protein. The ETF is a heterodimer of α- and β-subunits. Lysine residues 199 and 202 of mature ETFβ are almost completely trimethylated in bovine heart mitochondria, whereas ETFα is not methylated. The enzyme responsible for the modifications was identified as methyltransferase-like protein 20 (METTL20). In human 143B cells, the methylation of ETFβ is less extensive and is diminished further by suppression of METTL20. Tagged METTL20 expressed in HEK293T cells specifically associates with the ETF and promotes the trimethylation of ETFβ lysine residues 199 and 202. ETF serves as a mobile electron carrier linking dehydrogenases involved in fatty acid oxidation and one-carbon metabolism to the membrane-associated ubiquinone pool. The methylated residues in ETFβ are immediately adjacent to a protein loop that recognizes and binds to the dehydrogenases. Suppression of trimethylation of ETFβ in mouse C2C12 cells oxidizing palmitate as an energy source reduced the consumption of oxygen by the cells. These experiments suggest that the oxidation of fatty acids in mitochondria and the passage of electrons via the ETF may be controlled by modulating the protein-protein interactions between the reduced dehydrogenases and the β-subunit of the ETF by trimethylation of lysine residues. METTL20 is the first lysine methyltransferase to be found to be associated with mitochondria. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

76 FR 68521 - Self-Regulatory Organizations; NYSE Arca, Inc.; Order Granting Approval of Proposed Rule Change...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-04

... Managed Fund Shares of TrimTabs Float Shrink ETF under NYSE Arca Equities Rule 8.600; Correction November... Rule Change to List and Trade Managed Fund Shares of TrimTabs Float Shrink ETF under NYSE Arca Equities...

5,5'-Dithiobis-(2-nitrobenzoic acid) as a probe for a non-essential cysteine residue at the medium chain acyl-coenzyme A dehydrogenase binding site of the human 'electron transferring flavoprotein' (ETF).

PubMed

Parker, A; Engel, P C

1999-01-01

Human 'electron transferring flavoprotein' (ETF) was inactivated by the thiol-specific reagent 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB). The kinetic profile showed the reaction followed pseudo-first-order kinetics during the initial phase of inactivation. Monitoring the release of 5-thio-2-nitrobenzoate (TNB) showed that modification of 1 cysteine residue was responsible for the loss of activity. The inactivation of ETF by DTNB could be reversed upon incubation with thiol-containing reagents. The loss of activity was prevented by the inclusion of medium chain acyl-CoA dehydrogenase (MCAD) and octanoyl-CoA. Cyanolysis of the DTNB modified-ETF with KCN led to the release of TNB accompanied presumably by the formation of the thio-cyano enzyme and with almost full recovery of activity. Conservation studies and the lack of 100% inactivation, however, suggested that this cysteine residue is not essential for the interaction with MCAD.

Dynamic environmental control mechanisms for pneumatic foil constructions

NASA Astrophysics Data System (ADS)

Flor, Jan-Frederik; Wu, Yupeng; Beccarelli, Paolo; Chilton, John

2017-11-01

Membrane and foil structures have become over the last decades an attractive alternative to conventional materials and building systems with increasing implementation in different typologies and scale. The development of transparent, light, flexible and resistant materials like Ethylene Tetrafluoroethylene (ETFE) has triggered a rethinking of the building envelope in the building industry towards lightweight systems. ETFE foil cushions have proven to fulfil the design requirements in terms of structural efficiency and aesthetic values. But the strategies to satisfy increasing demands of energy efficiency and comfort conditions are still under development. The prediction and manipulation of the thermo-optical behaviour of ETFE foil cushion structures currently remain as one of the main challenges for designers and manufacturers. This paper reviews ongoing research regarding the control of the thermo-optical performance of ETFE cushion structures and highlights challenges and possible improvements. An overview of different dynamic and responsive environmental control mechanisms for multilayer foil constructions is provided and the state of the art in building application outlined by the discussion of case studies.

Fermionic ground state at unitarity and Haldane exclusion statistics

NASA Astrophysics Data System (ADS)

Bhaduri, R. K.; Murthy, M. V. N.; Brack, M.

2008-06-01

We consider a few-particle system of trapped neutral fermionic atoms at ultra-low temperatures, with the attractive interaction tuned to Feshbach resonance. We calculate the energies and the spatial densities of the few-body systems using a generalization of the extended Thomas-Fermi (ETF) method, and assuming the particles obey the Haldane-Wu fractional exclusion statistics (FES) at unitarity. This method is different from the scaled ETF version given by Chang and Bertsch (2007 Phys. Rev. A 76 021603). Our semiclassical FES results are consistent with the Monte Carlo calculations of the above authors, but can hardly be distinguished from their overall scaling of the ETF result at unitarity.

Eigengaps for Hub-Dominant Matrices

DTIC Science & Technology

2011-09-01

frame ( ETF ) if it satisfies two conditions: (i) For some nonnegative , we have jhvk, vjij¼ when 1 k5jN and (ii) PN k¼1 vkv t k ¼ Ns Id, when Id...and ETF lead to the following results. 1238 L. Shen and B.W. Suter D ow nl oa de d by [ L ix in S he n] a t 0 4: 58 0 8 Ju ly 2 01 1 13...1 of A forms an ETF , then (i) kamk 1; (ii) ¼ (m 2) þ 1, where is the constant in (23) and ¼ jhai, ajij for all 1 i5jm 1 and (iii) 1m2

Static Dissipative Cable Ties, Such as for Radiation Belt Storm Probes

NASA Technical Reports Server (NTRS)

Langley, Patrick T. (Inventor); Siddique, Fazle E. (Inventor)

2013-01-01

Methods of cyclically heating and cooling an article formed of a static dissipative ETFE resin, such as to reduce an electrical resistivity and/or to increase a tensile strength of the article, and methods of irradiating an article formed of a static dissipative ETFE resin, such as to increase a tensile strength of the article. Also disclosed herein are articles formed of a static dissipative ETFE resin, and processed in accordance with methods disclosed herein. Such an article may include, for example and without limitation, a cable strap to wrap, support, and/or secure one or more wires or cables, such as a cable tie.

ETF Work Programme, 2003.

ERIC Educational Resources Information Center

European Training Foundation, Turin (Italy).

This document describes the proposed 2003 activities of the European Training Foundation (ETF) to support vocational education and training reform in non-European Union (EU) countries in the context of the EU's external relations program. The activities listed cover these four main groups of countries across three continents: the Mediterranean…

Work Programme, 2001-2003.

ERIC Educational Resources Information Center

European Training Foundation, Turin (Italy).

This document provides a general overview, in broad terms, of the activities of the European Training Foundation (ETF) planned for 2001 and beyond. Section 1 presents ETF activities under four main headings: obligatory activities; support to the European Commission; information provision and analysis; and development activities. It also provides a…

Electron irradiation effects on partially fluorinated polymer films: Structure-property relationships

NASA Astrophysics Data System (ADS)

Nasef, Mohamed Mahmoud; Dahlan, Khairul Zaman M.

2003-04-01

The effects of electron beam irradiation on two partially fluorinated polymer films i.e. poly(vinylidene fluoride) (PVDF) and poly(ethylene-tetrafluoroethylene) copolymer (ETFE) are studied at doses ranging from 100 to 1200 kGy in air at room temperature. Chemical structure, thermal and mechanical properties of irradiated films are investigated. FTIR show that both PVDF and ETFE films undergo similar changes in their chemical structures including the formation of carbonyl groups and double bonding. The changes in melting and crystallisation temperatures ( Tm and Tc) in both irradiated films are functions of irradiation dose and reflect the disorder in the chemical structure caused by the competition between crosslinking and chain scission. The heat of melting (Δ Hm) and the degree of crystallinity ( Xc) of PVDF films show no significant changes with the dose increase, whereas those of ETFE films are reduced rapidly after the first 100 kGy. The tensile strength of PVDF films is improved by irradiation compared to its rapid deterioration in ETFE films, which stemmed from the degradation prompted by the presence of radiation sensitive tetrafluoroethylene (TFE) comonomer units. The elongation at break of both films drops gradually with the dose increase indicating the formation of predominant crosslinked structures at high doses. However, the response of each polymer to crosslinking and main chain scission at various irradiation doses varies from PVDF to ETFE films.

U.S. Navy Marine Climatic Atlas of the World. Volume 6. Arctic Ocean

DTIC Science & Technology

1963-02-01

commonly used trade ch or closely parallel coastlines. difTerences will be noted between isopleth values graphs in the same area. Most discrepancies...95% H»«fil range e 1,500 Uf. otd ihe M% 84% range e 1,000 ( etf ) Mean and langei a’ height are omrtled «hen data loi o level OfB mining at

78 FR 20149 - Sage Quant Management LLC, et al.; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-03

... Quant Management LLC, et al.; Notice of Application March 28, 2013. AGENCY: Securities and Exchange...)(1)(A) and 12(d)(1)(B) of the Act. Applicants: Sage Quant Management LLC (``Adviser''), and Sage Quant ETF Trust (``Trust'') and ETF Distributors LLC. SUMMARY: Summary of Application: Applicants...

77 FR 34095 - Federated Investment Management Company and Federated ETF Trust; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-08

... Investment Management Company and Federated ETF Trust; Notice of Application June 1, 2012. AGENCY: Securities... (e) certain registered management investment companies and unit investment trusts outside of the same... Daniele Marchesani, Branch Chief, at (202) 551-6821 (Division of Investment Management, Office of...

The New Geography: The Map, The Satellite, and The Computer

DTIC Science & Technology

2011-03-23

GDP Ratio To Evaluate Country ETFs ,” September 24, 2007, linked from Seeking Alpha Website, http://seekingalpha.com/article/47992-using-trade-to- gdp...ratio-to-evaluate-country- etfs , (accessed February 18, 2011). 51For additional information and charts on oil and energy refer to the U.S. Energy

78 FR 63522 - Syntax Analytics, LLC and Syntax ETF Trust; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-24

... Analytics, LLC and Syntax ETF Trust; Notice of Application October 18, 2013. AGENCY: Securities and Exchange... Trust (``Trust''). Summary of Application: Applicants request an order that permits: (a) Actively... unit investment trusts outside of the same group of investment companies as the series to acquire...

The attenuation of microorganisms in on-site wastewater effluent discharged into highly permeable subsoils

NASA Astrophysics Data System (ADS)

O'Luanaigh, N. D.; Gill, L. W.; Misstear, B. D. R.; Johnston, P. M.

2012-11-01

An extensive field study on percolation areas receiving both septic tank and secondary treated on-site effluents from single houses in Ireland was carried out to investigate the attenuation capacity of highly permeable subsoils with respect to E. coli bacteria and spiked bacteriophages (MS2, ΦX174 and PR772). The development of biomats across the percolation areas receiving the secondary effluent was restricted compared to the percolation area receiving septic tank effluent, promoting a much higher areal hydraulic loading which created significant differences in the potential microbiological loading to groundwater. Greatest E. coli removal in the subsoil occurred within the first 0.35 m of unsaturated subsoil for all effluent types. Analysis showed, however, that more evidence of faecal contamination occurred at depth in the subsoils receiving secondary treated effluents than that receiving septic tank effluent, despite the lower bacterial influent load. All three bacteriophages were reduced to their minimum detection limit (< 10 PFU/mL) at a depth of 0.95 m below the percolation trenches receiving septic tank effluent, although isolated incidences of ΦX174 and PR772 were measured below one trench. However again, slightly higher breakthroughs of MS2 and PR772 contamination were detected at the same depth under the trenches receiving secondary treated effluent.

Nutrient loading on subsoils from on-site wastewater effluent, comparing septic tank and secondary treatment systems.

PubMed

Gill, L W; O'Luanaigh, N; Johnston, P M; Misstear, B D R; O'Suilleabhain, C

2009-06-01

The performance of six separate percolation areas was intensively monitored to ascertain the attenuation effects of unsaturated subsoils with respect to on-site wastewater effluent: three sites receiving septic tank effluent, the other three sites receiving secondary treated effluent. The development of a biomat across the percolation areas receiving secondary treated effluent was restricted on these sites compared to those sites receiving septic tank effluent and this created significant differences in terms of the potential nitrogen loading to groundwater. The average nitrogen loading per capita at 1.0m depth of unsaturated subsoil equated to 3.9 g total-N/d for the sites receiving secondary treated effluent, compared to 2.1 g total-N/d for the sites receiving septic tank effluent. Relatively high nitrogen loading was, however, found on the septic tank sites discharging effluent into highly permeable subsoil that counteracted any significant denitrification. Phosphorus removal was generally very good on all of the sites although a clear relationship to the soil mineralogy was determined.

Analytical mass formula and nuclear surface properties in the ETF approximation. Part I: symmetric nuclei

NASA Astrophysics Data System (ADS)

Aymard, François; Gulminelli, Francesca; Margueron, Jérôme

2016-08-01

The problem of determination of nuclear surface energy is addressed within the framework of the extended Thomas Fermi (ETF) approximation using Skyrme functionals. We propose an analytical model for the density profiles with variationally determined diffuseness parameters. In this first paper, we consider the case of symmetric nuclei. In this situation, the ETF functional can be exactly integrated, leading to an analytical formula expressing the surface energy as a function of the couplings of the energy functional. The importance of non-local terms is stressed and it is shown that they cannot be deduced simply from the local part of the functional, as it was suggested in previous works.

77 FR 74884 - Cambria Investment Management, L.P. and Cambria ETF Trust; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-18

... Investment Management, L.P. and Cambria ETF Trust; Notice of Application December 12, 2012. AGENCY... request an order that permits: (a) Actively-managed series of certain open-end management investment... registered management investment companies and unit investment trusts outside of the same group of investment...

78 FR 79519 - IndexIQ ETF Trust, et al.; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-30

... SECURITIES AND EXCHANGE COMMISSION [Investment Company Act Release No. 30844; 812-14153] IndexIQ ETF Trust, et al.; Notice of Application December 23, 2013. AGENCY: Securities and Exchange Commission... Company Act of 1940 (the ``1940 Act'') for exemptions from sections 12(d)(1)(A), (B), and (C) of the 1940...

75 FR 14478 - Self-Regulatory Organizations; The Options Clearing Corporation; Notice of Filing of Proposed...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-25

... SECURITIES AND EXCHANGE COMMISSION [Release No. 34-61692; File No. SR-OCC-2010-03] Self[dash]Regulatory Organizations; The Options Clearing Corporation; Notice of Filing of Proposed Rule Change Relating to ETFS Palladium Shares and ETFS Platinum Shares Correction In notice document 2010-5914 beginning...

75 FR 55619 - Self-Regulatory Organizations; The Options Clearing Corporation; Notice of Filing of Proposed...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-13

... Numerical Simulations Risk Management Methodology September 7, 2010. Pursuant to Section 19(b)(1) of the... for incorporation in the System for Theoretical Analysis and Numerical Simulations (``STANS'') risk... ETFs \\3\\ in the STANS margin calculation process.\\4\\ When OCC began including common stock and ETFs in...

Task-Technology Fit Assessment of an Expertise Transfer System

DTIC Science & Technology

2009-03-01

Air Education and Training Command In Partial Fulfillment of the Requirements for the Degree of Master of Science in Information Resource Management...Transfer Forum (ETF) developed by the Oklahoma State University for the Defense Ammunition Center’s quality assurance personnel. The preliminary findings...Technology-to-Performance Chain (TPC) ....................................................................13 Expertise Transfer Forum (ETF

78 FR 46381 - Calamos Advisors LLC and Calamos ETF Trust; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-31

... Advisors LLC and Calamos ETF Trust; Notice of Application July 24, 2013. AGENCY: Securities and Exchange... Trust (`Trust). Summary of Application: Applicants request an order that permits: (a) Actively-managed... investment trusts outside of the same group of investment companies as the series to acquire Shares. Filing...

ETFE polymer bombarded with 1 MeV proton

NASA Astrophysics Data System (ADS)

Parada, M. A.; de Almeida, A.; Muntele, I.; Muntele, C.; Delalez, N.; Ila, D.

2005-12-01

The ethylenetetrafluoroethylene (ETFE) is a polymer formed by alternating ethylene and tetrafluoroethylene segments. It has high impact resistance and useful mechanical properties. ETFE can be used as components of pumps, valves, tie wraps, and electrical components. It can also be applied in the field of medical physics as intra venous catheters and as radiation dosimeter. When a material is exposed to the ionizing radiation, it suffers damage that depends on the type, energy and intensity of the radiation. In order to determine the radiation damage mechanism, ETFE films were bombarded with 1 MeV protons to the fluence between 1 × 1011 and 1 × 1016 protons/cm2 and the chemical species emitted during the bombardment were measured with residual gas analysis (RGA) and show that HF gas is the entity preferentially emitted. Optical absorption photospectrometry (OAP) and attenuated total reflectometry fourier transform infrared (ATR-FTIR) shows quantitative chemical evidence of the damage. Our results show that damage is detectable at low proton fluence, but damage that can compromise the application in dosimetry occurs only for fluence greater than 1014 protons/cm2.

The light ion pulsed power induction accelerator for ETF

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mazarakis, M.G.; Olson, R.E.; Olson, C.L.

1994-12-31

Our Engineering Test Facility (ETF) driver concept is based on HERMES III and RHEPP technologies. Actually, it is a scaled-down version of the LMF design incorporating repetition rate capabilities of up to 10 Hz CW. The preconceptual design presented here provides 200-TW peak power to the ETF target during 10 ns, equal to 2-MJ total ion beam energy. Linear inductive voltage addition driving a self-magnetically insulated transmission line (MITL) is utilized to generate the 36-MV peak voltage needed for lithium ion beams. The {approximately} 3-MA ion current is achieved by utilizing many accelerating modules in parallel. Since the current permore » module is relatively modest ({approximately}300 kA), two-stage or one-stage extraction diodes can be utilized for the generation of singly charged lithium ions. The accelerating modules are arranged symmetrically around the fusion chamber in order to provide uniform irradiation onto the ETF target. In addition, the modules are fired in a programmed sequence in order to generate the optimum power pulse shape onto the target. This design utilizes RHEPP accelerator modules as the principal power source.« less

The development of a code of practice for single house on-site wastewater treatment in Ireland.

PubMed

Gill, L W

2011-01-01

The performance of six separate percolation areas was intensively monitored to ascertain the attenuation effects of unsaturated subsoils with respect to on-site wastewater effluent: three sites receiving septic tank effluent, the other three sites receiving secondary treated effluent. The development of a biomat across the percolation areas receiving secondary treated effluent was restricted on these sites compared to those sites receiving septic tank effluent. This created significant differences in terms of the hydraulic loading on the percolation areas with implications for the transport and attenuation of indicator microorganisms and nitrogen down through the subsoils and into the groundwater. The results of this work have formed a large input into the production of a new Code of Practice Wastewater Treatment and Disposal Systems Serving Single Houses. This has led to changes in the design of on-site hydraulic loading from 180 L per capita per day (L/c.d) down to 150 L/c.d. The range of acceptable subsoils receiving septic tank effluent has narrowed for more highly permeable subsoils following a series of tracer studies using bacteriophages. However, the range has been extended for lower permeability subsoils (range 0.08 down to 0.06 m/d) receiving secondary treated effluent in order to encourage the effluent to spread further along the trenches. The maximum individual length of percolation trenches receiving secondary effluent has also been reduced to 10 m to encourage dispersion on a wider area. This paper thus highlights how research can directly feed into a Code of Practice.

Secondary Waste Form Down-Selection Data Package—Fluidized Bed Steam Reforming Waste Form

DOE Office of Scientific and Technical Information (OSTI.GOV)

Qafoku, Nikolla; Westsik, Joseph H.; Strachan, Denis M.

2011-09-12

The Hanford Site in southeast Washington State has 56 million gallons of radioactive and chemically hazardous wastes stored in 177 underground tanks (ORP 2010). The U.S. Department of Energy (DOE), Office of River Protection (ORP), through its contractors, is constructing the Hanford Tank Waste Treatment and Immobilization Plant (WTP) to convert the radioactive and hazardous wastes into stable glass waste forms for disposal. Within the WTP, the pretreatment facility will receive the retrieved waste from the tank farms and separate it into two treated process streams. These waste streams will be vitrified, and the resulting waste canisters will be sentmore » to offsite (high-level waste [HLW]) and onsite (immobilized low-activity waste [ILAW]) repositories. As part of the pretreatment and ILAW processing, liquid secondary wastes will be generated that will be transferred to the Effluent Treatment Facility (ETF) on the Hanford Site for further treatment. These liquid secondary wastes will be converted to stable solid waste forms that will be disposed of in the Integrated Disposal Facility (IDF). To support the selection of a waste form for the liquid secondary wastes from WTP, Washington River Protection Solutions (WRPS) has initiated secondary waste form testing work at Pacific Northwest National Laboratory (PNNL). In anticipation of a down-selection process for a waste form for the Solidification Treatment Unit to be added to the ETF, PNNL is developing data packages to support that down-selection. The objective of the data packages is to identify, evaluate, and summarize the existing information on the four waste forms being considered for stabilizing and solidifying the liquid secondary wastes. At the Hanford Site, the FBSR process is being evaluated as a supplemental technology for treating and immobilizing Hanford LAW radioactive tank waste and for treating secondary wastes from the WTP pretreatment and LAW vitrification processes.« less

Clear relationship between ETF/ETFDH genotype and phenotype in patients with multiple acyl-CoA dehydrogenation deficiency.

PubMed

Olsen, Rikke K J; Andresen, Brage S; Christensen, Ernst; Bross, Peter; Skovby, Flemming; Gregersen, Niels

2003-07-01

Mutations in electron transfer flavoprotein (ETF) and its dehydrogenase (ETFDH) are the molecular basis of multiple acyl-CoA dehydrogenation deficiency (MADD), an autosomal recessively inherited and clinically heterogeneous disease that has been divided into three clinical forms: a neonatal-onset form with congenital anomalies (type I), a neonatal-onset form without congenital anomalies (type II), and a late-onset form (type III). To examine whether these different clinical forms could be explained by different ETF/ETFDH mutations that result in different levels of residual ETF/ETFDH enzyme activity, we have investigated the molecular genetic basis for disease development in nine patients representing the phenotypic spectrum of MADD. We report the genomic structures of the ETFA, ETFB, and ETFDH genes and the identification and characterization of seven novel and three previously reported disease-causing mutations. Our molecular genetic investigations of these nine patients are consistent with three clinical forms of MADD showing a clear relationship between the nature of the mutations and the severity of disease. Interestingly, our data suggest that homozygosity for two null mutations causes fetal development of congenital anomalies resulting in a type I disease phenotype. Even minute amounts of residual ETF/ETFDH activity seem to be sufficient to prevent embryonic development of congenital anomalies giving rise to type II disease. Overexpression studies of an ETFB-D128N missense mutation identified in a patient with type III disease showed that the residual activity of the mutant enzyme could be rescued up to 59% of that of wild-type activity when ETFB-D128N-transformed E. coli cells were grown at low temperature. This indicates that the effect of the ETF/ETFDH genotype in patients with milder forms of MADD, in whom residual enzyme activity allows modulation of the enzymatic phenotype, may be influenced by environmental factors like cellular temperature. Copyright 2003 Wiley-Liss, Inc.

Comparison of effects of dry versus wet swallowing on Eustachian tube function via a nine-step inflation/deflation test.

PubMed

Adali, M Kemal; Uzun, Cem

2005-09-01

The aim of the present study is to evaluate the effect of swallowing type (dry versus wet) on the outcome of a nine-step inflation/deflation tympanometric Eustachian tube function (ETF) test in healthy adults. Fourteen normal healthy volunteers, between 19 and 28 years of age, were included in the study. The nine-step test was performed in two different test procedures: (1) test with dry swallows (dry test procedure) and (2) test with liquid swallows (wet test procedure). If the equilibration of middle-ear (ME) pressure was successful in all the steps of the nine-step test, ETF was considered 'Good'. Otherwise, the test was considered 'Poor', and the test was repeated at a second session. In the dry test procedure, ETF was 'Good' in 21 ears at the first session and in 24 ears after the second session (p > 0.05). However, in the wet test procedure, ETF was 'Good' in 13 ears at the first session and in 21 ears after the second session (p < 0.05). At the first session, ETF was 'Good' in 21 and 13 ears in the dry and wet test procedures, respectively. The difference was statistically significant (p < 0.05). However, after the second session, the overall number of ears with 'Good' tubal function was almost the same in both test procedures (24 ears at dry test procedures versus 21 ears at wet test procedures;p > 0.05). Dry swallowing seems to be more effective for the equilibration of ME pressure. Thus, a single-session dependent evaluation of ETF may be efficient for the dry test procedure of the nine-step test. Swallowing with water may be easier for subjects, but a repetition of the test at a second session may be necessary when the test result is 'Poor'.

Mutations at the flavin binding site of ETF:QO yield a MADD-like severe phenotype in Drosophila.

PubMed

Alves, Ema; Henriques, Bárbara J; Rodrigues, João V; Prudêncio, Pedro; Rocha, Hugo; Vilarinho, Laura; Martinho, Rui G; Gomes, Cláudio M

2012-08-01

Following a screening on EMS-induced Drosophila mutants defective for formation and morphogenesis of epithelial cells, we have identified three lethal mutants defective for the production of embryonic cuticle. The mutants are allelic to the CG12140 gene, the fly homologue of electron transfer flavoprotein:ubiquinone oxidoreductase (ETF:QO). In humans, inherited defects in this inner membrane protein account for multiple acyl-CoA dehydrogenase deficiency (MADD), a metabolic disease of β-oxidation, with a broad range of clinical phenotypes, varying from embryonic lethal to mild forms. The three mutant alleles carried distinct missense mutations in ETF:QO (G65E, A68V and S104F) and maternal mutant embryos for ETF:QO showed lethal morphogenetic defects and a significant induction of apoptosis following germ-band elongation. This phenotype is accompanied by an embryonic accumulation of short- and medium-chain acylcarnitines (C4, C8 and C12) as well as long-chain acylcarnitines (C14 and C16:1), whose elevation is also found in severe MADD forms in humans under intense metabolic decompensation. In agreement the ETF:QO activity in the mutant embryos is markedly decreased in relation to wild type activity. Amino acid sequence analysis and structural mapping into a molecular model of ETF:QO show that all mutations map at FAD interacting residues, two of which at the nucleotide-binding Rossmann fold. This structural domain is composed by a β-strand connected by a short loop to an α-helix, and its perturbation results in impaired cofactor association via structural destabilisation and consequently enzymatic inactivation. This work thus pinpoints the molecular origins of a severe MADD-like phenotype in the fruit fly and establishes the proof of concept concerning the suitability of this organism as a potential model organism for MADD. © 2012 Elsevier B.V. All rights reserved.

Updated Liquid Secondary Waste Grout Formulation and Preliminary Waste Form Qualification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Saslow, Sarah A.; Um, Wooyong; Russell, Renee L.

This report describes the results from liquid secondary waste grout (LSWG) formulation and cementitious waste form qualification tests performed by Pacific Northwest National Laboratory (PNNL) for Washington River Protection Solutions, LLC (WRPS). New formulations for preparing a cementitious waste form from a high-sulfate liquid secondary waste stream simulant, developed for Effluent Management Facility (EMF) process condensates merged with low activity waste (LAW) caustic scrubber, and the release of key constituents (e.g. 99Tc and 129I) from these monoliths were evaluated. This work supports a technology development program to address the technology needs for Hanford Site Effluent Treatment Facility (ETF) liquid secondarymore » waste (LSW) solidification and supports future Direct Feed Low-Activity Waste (DFLAW) operations. High-priority activities included simulant development, LSWG formulation, and waste form qualification. The work contained within this report relates to waste form development and testing and does not directly support the 2017 integrated disposal facility (IDF) performance assessment (PA). However, this work contains valuable information for use in PA maintenance past FY17, and for future waste form development efforts. The provided data should be used by (i) cementitious waste form scientists to further understanding of cementitious dissolution behavior, (ii) IDF PA modelers who use quantified constituent leachability, effective diffusivity, and partitioning coefficients to advance PA modeling efforts, and (iii) the U.S. Department of Energy (DOE) contractors and decision makers as they assess the IDF PA program. The results obtained help fill existing data gaps, support final selection of a LSWG waste form, and improve the technical defensibility of long-term waste form performance estimates.« less

Removal of heavy metals from tannery effluents of Ambur industrial area, Tamilnadu by Arthrospira (Spirulina) platensis.

PubMed

Balaji, S; Kalaivani, T; Rajasekaran, C; Shalini, M; Vinodhini, S; Priyadharshini, S Sunitha; Vidya, A G

2015-06-01

The present study was carried out with the tannery effluent contaminated with heavy metals collected from Ambur industrial area to determine the phycoremediation potential of Arthrospira (Spirulina) platensis. Two different concentrations (50 and 100 %) of heavy metals containing tannery effluent treated with A. platensis were analysed for growth, absorption spectra, biochemical properties and antioxidant enzyme activity levels. The effluent treatments revealed dose-dependent decrease in the levels of A. platensis growth (65.37 % for 50 % effluent and 49.32 % for 100 % effluent), chlorophyll content (97.43 % for 50 % effluent and 71.05 % for 100 % effluent) and total protein content (82.63 % for 50 % effluent and 62.10 % for 100 % effluent) that leads to the reduction of total solids, total dissolved solids and total suspended solids. A. platensis with lower effluent concentration was effective than at higher concentration. Treatment with the effluent also resulted in increased activity levels of antioxidant enzymes, such as superoxide dismutase (14.58 units/g fresh weight for 50 % and 24.57 units/g fresh weight for 100 %) and catalase (0.963 units/g fresh weight for 50 % and 1.263 units/g fresh weight for 100 %). Furthermore, heavy metal content was determined using atomic absorption spectrometry. These results indicated that A. platensis has the ability to combat heavy metal stress by the induction of antioxidant enzymes demonstrating its potential usefulness in phycoremediation of tannery effluent.

75 FR 81977 - Order Exempting the Trading and Clearing of Certain Products Related to the CBOE Gold ETF...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-29

... derivatives transaction execution facility for transactions for future delivery in any commodity under section... categorical Section 4(c) exemption to permit options and futures on shares of all or some precious metal commodity-based ETFs to be traded and cleared as options on securities and security futures, respectively...

75 FR 9981 - Self-Regulatory Organizations; The Options Clearing Corporation; Order Approving Proposed Rule...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-04

... securities options or the clearing of such futures as security futures constitutes a violation of the CEA. \\3... same as the options and security futures on SPDR Gold Shares, iShares COMEX Gold Shares, and iShares... to help clarify that options and security futures on ETFS Physical Swiss Gold Shares and ETFS...

75 FR 14646 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-26

... Organizations; International Securities Exchange, LLC; Notice of Filing of Proposed Rule Change To List and... ``Commission'') authorized ISE to list and trade options on the SPDR Gold Trust,\\3\\ the iShares COMEX Gold... Exchange proposes to list and trade options on the ETFS Palladium Trust and the ETFS Platinum Trust. \\3...

76 FR 20779 - Self-Regulatory Organizations; The Options Clearing Corporation; Notice of Filing and Immediate...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-13

... Change To Provide Legal Certainty for the Trading of Futures on the CBOE Gold ETF Volatility Index April... CBOE Gold ETF Volatility Index (``GVZ Index''). II. Self-Regulatory Organization's Statement of the..., LLC (``CFE'') as an up-to-the-minute market estimate of the expected volatility of SPDR Gold Shares...

76 FR 23632 - Self-Regulatory Organizations; NYSE Amex LLC; Notice of Filing and Immediate Effectiveness of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-27

..., CBOE calculates the CBOE Gold ETF Volatility Index (``GVZ''), which is based on the VIX methodology applied to options on the SPDR Gold Trust (``GLD''). The current filing would permit $0.50 strike price... other exchange-traded fund (``ETF'') options. See Rule 903, Commentary .05 Volatility indexes are...

78 FR 79511 - IndexIQ ETF Trust, et al.; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-30

... SECURITIES AND EXCHANGE COMMISSION [Investment Company Act Release No. 30843; File No. 812-14053] IndexIQ ETF Trust, et al.; Notice of Application December 23, 2013. AGENCY: Securities and Exchange... Investment Company Act of 1940 (the ``Act'') for an exemption from sections 2(a)(32), 5(a)(1), 22(d), and 22...

78 FR 29399 - ERNY Financial ETF Trust and ERNY Financial Advisors, LLC; Notice of Application

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-20

... SECURITIES AND EXCHANGE COMMISSION [Investment Company Act Release No. 30514; 812-14146] ERNY Financial ETF Trust and ERNY Financial Advisors, LLC; Notice of Application May 13, 2013. AGENCY: Securities and Exchange Commission (``Commission''). ACTION: Notice of an application for an order under section 6(c) of the Investment Company Act of 1940...

77 FR 76099 - Yorkville ETF Trust and Yorkville ETF Advisors, LLC; Notice of Application

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2012-12-26

... given trading day, or from day to day, such variances occur as a result of third party market forces... that the proposed distribution system will be orderly because competitive forces will ensure that the... include concerns about undue influence by a fund of funds over underlying funds, excessive layering of...

77 FR 74707 - Self-Regulatory Organizations; NYSE Arca, Inc.; Order Approving a Proposed Rule Change To List...

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2012-12-17

... the Pring Turner Business Cycle ETF Under NYSE Arca Equities Rule 8.600 December 11, 2012. On October... Pring Turner Business Cycle ETF (``Fund''). The proposed rule change was published for comment in the... proprietary business cycle research, the Sub-Adviser proactively will change the Fund's asset allocation and...

Mean electromotive force generated by asymmetric fluid flow near the surface of earth's outer core

NASA Astrophysics Data System (ADS)

Bhattacharyya, Archana

1992-10-01

The phi component of the mean electromotive force, (ETF) generated by asymmetric flow of fluid just beneath the core-mantle boundary (CMB), is obtained using a geomagnetic field model. This analysis is based on the supposition that the axisymmetric part of fluid flow beneath the CMB is tangentially geostrophic and toroidal. For all the epochs studied, the computed phi component is stronger in the Southern Hemisphere than that in the Northern Hemisphere. Assuming a linear relationship between (ETF) and the azimuthally averaged magnetic field (AAMF), the only nonzero off-diagonal components of the pseudotensor relating ETF to AAMF, are estimated as functions of colatitude, and the physical implications of the results are discussed.

Optical Characteristic Change of Transparent Film Due to Combined Exposure to Atomic Oxygen and Ultraviolet Ray

NASA Astrophysics Data System (ADS)

Okumura, Teppei; Hosoda, Satoshi; Kim, Jeongho; Iwata, Minoru; Cho, Mengu

ETFE film covered solar array was developed for arc mitigation in LEO environment. We used ETFE film because its transmittance is excellent at the wavelengths from 300 to 900nm. In LEO, however, the film will encounter serious space environment, such as AO and UV. Electrical power output may decrease due to degradation of transmittance. To study the effects of AO and UV radiation on ETFE film, we used a combined space effect test facility at JAXA. Although the film color turned white after the test, there was no change of film transmittance. Because of AO-induced erosion, texture structure was formed on the film surface, that trapped incident light and made it pass the film after scattering.

Computational analysis of a novel mutation in ETFDH gene highlights its long-range effects on the FAD-binding motif.

PubMed

Er, Tze-Kiong; Chen, Chih-Chieh; Liu, Yen-Yi; Chang, Hui-Chiu; Chien, Yin-Hsiu; Chang, Jan-Gowth; Hwang, Jenn-Kang; Jong, Yuh-Jyh

2011-10-21

Multiple acyl-coenzyme A dehydrogenase deficiency (MADD) is an autosomal recessive disease caused by the defects in the mitochondrial electron transfer system and the metabolism of fatty acids. Recently, mutations in electron transfer flavoprotein dehydrogenase (ETFDH) gene, encoding electron transfer flavoprotein:ubiquinone oxidoreductase (ETF:QO) have been reported to be the major causes of riboflavin-responsive MADD. To date, no studies have been performed to explore the functional impact of these mutations or their mechanism of disrupting enzyme activity. High resolution melting (HRM) analysis and sequencing of the entire ETFDH gene revealed a novel mutation (p.Phe128Ser) and the hotspot mutation (p.Ala84Thr) from a patient with MADD. According to the predicted 3D structure of ETF:QO, the two mutations are located within the flavin adenine dinucleotide (FAD) binding domain; however, the two residues do not have direct interactions with the FAD ligand. Using molecular dynamics (MD) simulations and normal mode analysis (NMA), we found that the p.Ala84Thr and p.Phe128Ser mutations are most likely to alter the protein structure near the FAD binding site as well as disrupt the stability of the FAD binding required for the activation of ETF:QO. Intriguingly, NMA revealed that several reported disease-causing mutations in the ETF:QO protein show highly correlated motions with the FAD-binding site. Based on the present findings, we conclude that the changes made to the amino acids in ETF:QO are likely to influence the FAD-binding stability.

Computational analysis of a novel mutation in ETFDH gene highlights its long-range effects on the FAD-binding motif

PubMed Central

2011-01-01

Background Multiple acyl-coenzyme A dehydrogenase deficiency (MADD) is an autosomal recessive disease caused by the defects in the mitochondrial electron transfer system and the metabolism of fatty acids. Recently, mutations in electron transfer flavoprotein dehydrogenase (ETFDH) gene, encoding electron transfer flavoprotein:ubiquinone oxidoreductase (ETF:QO) have been reported to be the major causes of riboflavin-responsive MADD. To date, no studies have been performed to explore the functional impact of these mutations or their mechanism of disrupting enzyme activity. Results High resolution melting (HRM) analysis and sequencing of the entire ETFDH gene revealed a novel mutation (p.Phe128Ser) and the hotspot mutation (p.Ala84Thr) from a patient with MADD. According to the predicted 3D structure of ETF:QO, the two mutations are located within the flavin adenine dinucleotide (FAD) binding domain; however, the two residues do not have direct interactions with the FAD ligand. Using molecular dynamics (MD) simulations and normal mode analysis (NMA), we found that the p.Ala84Thr and p.Phe128Ser mutations are most likely to alter the protein structure near the FAD binding site as well as disrupt the stability of the FAD binding required for the activation of ETF:QO. Intriguingly, NMA revealed that several reported disease-causing mutations in the ETF:QO protein show highly correlated motions with the FAD-binding site. Conclusions Based on the present findings, we conclude that the changes made to the amino acids in ETF:QO are likely to influence the FAD-binding stability. PMID:22013910

Hanford Facility dangerous waste permit application, liquid effluent retention facility and 200 area effluent treatment facility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Coenenberg, J.G.

1997-08-15

The Hanford Facility Dangerous Waste Permit Application is considered to 10 be a single application organized into a General Information Portion (document 11 number DOE/RL-91-28) and a Unit-Specific Portion. The scope of the 12 Unit-Specific Portion is limited to Part B permit application documentation 13 submitted for individual, `operating` treatment, storage, and/or disposal 14 units, such as the Liquid Effluent Retention Facility and 200 Area Effluent 15 Treatment Facility (this document, DOE/RL-97-03). 16 17 Both the General Information and Unit-Specific portions of the Hanford 18 Facility Dangerous Waste Permit Application address the content of the Part B 19 permit applicationmore » guidance prepared by the Washington State Department of 20 Ecology (Ecology 1987 and 1996) and the U.S. Environmental Protection Agency 21 (40 Code of Federal Regulations 270), with additional information needs 22 defined by the Hazardous and Solid Waste Amendments and revisions of 23 Washington Administrative Code 173-303. For ease of reference, the Washington 24 State Department of Ecology alpha-numeric section identifiers from the permit 25 application guidance documentation (Ecology 1996) follow, in brackets, the 26 chapter headings and subheadings. A checklist indicating where information is 27 contained in the Liquid Effluent Retention Facility and 200 Area Effluent 28 Treatment Facility permit application documentation, in relation to the 29 Washington State Department of Ecology guidance, is located in the Contents 30 Section. 31 32 Documentation contained in the General Information Portion is broader in 33 nature and could be used by multiple treatment, storage, and/or disposal units 34 (e.g., the glossary provided in the General Information Portion). Wherever 35 appropriate, the Liquid Effluent Retention Facility and 200 Area Effluent 36 Treatment Facility permit application documentation makes cross-reference to 37 the General Information Portion, rather than duplicating text. 38 39 Information provided in this Liquid Effluent Retention Facility and 40 200 Area Effluent Treatment Facility permit application documentation is 41 current as of June 1, 1997.« less

75 FR 69058 - Request for Comment on a Proposal to Exempt, Pursuant to the Authority in Section 4(c) of the...

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2010-11-10

... categorical Section 4(c) exemption to permit options and futures on shares of all or some precious metal commodity-based ETFs to be traded and cleared as options on securities and security futures, respectively... options and futures on shares of precious metal commodity- based ETFs. The Commission believes that...

78 FR 73911 - Self-Regulatory Organizations; BOX Options Exchange LLC; Notice of Filing of Proposed Rule Change...

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2013-12-09

... 3120 To Extend the Pilot Program That Eliminated the Position Limits for Options on SPDR S&P 500 ETF... extend the pilot program that eliminated the position limits for options on SPDR S&P 500 ETF (``SPY...- regulatory organizations (``SROs'') have adopted similar rules eliminating position limits on SPY and market...

75 FR 24769 - Self-Regulatory Organizations; NYSE Amex LLC; Notice of Filing of Proposed Rule Change Amending...

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2010-05-05

... Commission (``SEC'' or ``Commission'') authorized the Exchange to list and trade options on the SPDR Gold... ETFS Silver Trust (``SIVR'') and the ETFS Gold Trust (``SGOL'').\\6\\ Now, the Exchange proposes to list... trust or similar entity that holds a specified non-U.S. currency deposited with the trust or similar...

78 FR 70370 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing of Proposed Rule Change Relating...

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2013-11-25

... open-end investment company that issues Investment Company Units, listed and traded on the Exchange...-based, fixed income exchange-traded funds (``ETFs'') contained in NYSE Arca Equities Rule 5.2(j)(3... and traded in the U.S. on registered exchanges. While the Fund may invest in inverse ETFs, the Fund...

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... calculates the CBOE Gold ETF Volatility Index (``GVZ''), which is based on the VIX methodology applied to options on the SPDR Gold Trust (``GLD''). The current filing would permit $0.50 strike price intervals for... exchange-traded fund (``ETF'') options. See Rule 1012, Commentary .05(a)(iv). To the extent that the CBOE...

Young People Not in Employment, Education or Training (NEET): An Overview in ETF Partner Countries

ERIC Educational Resources Information Center

Bardak, Ummuhan; Maseda, Martiño Rubal; Rosso, Francesca

2015-01-01

This report provides the first analysis of young people who are not in employment, education or training (NEET) in the partner countries of the European Training Foundation (ETF), on the basis of available data, and includes a discussion on the advantages and disadvantages of using this analysis for policy interventions. Bearing in mind the…

76 FR 45899 - Self-Regulatory Organizations; NYSE Amex LLC; Notice of Filing and Immediate Effectiveness of...

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2011-08-01

...-traded fund (``SPY ETF''),\\4\\ which list and trade under the option symbol SPY, and to update the names... 500 ETF represents ownership in the SPDR S&P 500 Trust, a unit investment trust that generally... Stock\\SM\\ (former option symbol QQQQ\\SM\\). NASDAQ, Nasdaq- 100 Index, Nasdaq-100 Index Tracking Stock...

Quality and Quality Assurance in Vocational Education and Training in the Mediterranean Countries: Lessons from the European Approach

ERIC Educational Resources Information Center

Masson, Jean-Raymond; Baati, Mounir; Seyfried, Erwin

2010-01-01

This article reflects on the development of the European approach towards quality and quality assurance in vocational education and training (VET) and its relevance for VET reforms in the European Training Foundation (ETF) partner countries. The analysis is based on an ETF project conducted in 2007-2008 in the Mediterranean partner countries to…

75 FR 22889 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

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2010-04-30

... transactions. Currently, the First Trust ISE Water ETF (``FIW''), the Claymore China Technology ETF (``CQQQ''), the ProShares UltraPro Short Dow30 (``SDOW''), the ProShares UltraPro Dow30 (``UDOW''), the ProShares UltraPro Short MidCap400 (``SMDD''), the ProShares UltraPro MidCap400 (``UMDD''), the ProShares UltraPro...

75 FR 32531 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

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2010-06-08

.... Currently, the First Trust ISE Water ETF (``FIW''), the Claymore China Technology ETF (``CQQQ''), the ProShares UltraPro Short Dow30 (``SDOW''), the ProShares UltraPro Dow30 (``UDOW''), the ProShares UltraPro Short MidCap400 (``SMDD''), the ProShares UltraPro MidCap400 (``UMDD''), the ProShares UltraPro Short...

75 FR 14236 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

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... transactions. Currently, the First Trust ISE Water ETF (``FIW'') and the Claymore China Technology ETF (``CQQQ... options on the ProShares UltraPro Short Dow30 (``SDOW''), the ProShares UltraPro Dow30 (``UDOW''), the ProShares UltraPro Short MidCap400 (``SMDD''), the ProShares UltraPro MidCap400 (``UMDD''), the ProShares...

Scoping the parameter space for demo and the engineering test facility (ETF)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meier, Wayne R.

1999-01-19

In our IFE development plan, we have set a goal of building an Engineering Test Facility (ETF) for a total cost of $2B and a Demo for $3B. In Mike Campbell' s presentation at Madison, we included a viewgraph with an example Demo that had 80 to 250 MWe of net power and showed a plausible argument that it could cost less than $3B. In this memo, I examine the design space for the Demo and then briefly for the ETF. Instead of attempting to estimate the costs of the drivers, I pose the question in a way to definemore » R&D goals: As a function of key design and performance parameters, how much can the driver cost if the total facility cost is limited to the specified goal? The design parameters examined for the Demo included target gain, driver energy, driver efficiency, and net power output. For the ETF; the design parameters are target gain, driver energy, and target yield. The resulting graphs of allowable driver cost determine the goals that the driver R&D programs must seek to meet.« less

Differential impact of amino acids on OXPHOS system activity following carbohydrate starvation in Arabidopsis cell suspensions.

PubMed

Cavalcanti, João Henrique F; Quinhones, Carla G S; Schertl, Peter; Brito, Danielle S; Eubel, Holger; Hildebrandt, Tatjana; Nunes-Nesi, Adriano; Braun, Hans-Peter; Araújo, Wagner L

2017-12-01

Plant respiration mostly depends on the activity of glycolysis and the oxidation of organic acids in the tricarboxylic acid cycle to synthesize ATP. However, during stress situations plant cells also use amino acids as alternative substrates to donate electrons through the electron-transfer flavoprotein (ETF)/ETF:ubiquinone oxidoreductase (ETF/ETFQO) complex to the mitochondrial electron transport chain (mETC). Given this, we investigated changes of the oxidative phosphorylation (OXPHOS) system in Arabidopsis thaliana cell culture under carbohydrate starvation supplied with a range of amino acids. Induction of isovaleryl-CoA dehydrogenase (IVDH) activity was observed under carbohydrate starvation which was associated with increased amounts of IVDH protein detected by immunoblotting. Furthermore, activities of the protein complexes of the mETC were reduced under carbohydrate starvation. We also observed that OXPHOS system activity behavior is differently affected by different amino acids and that proteins associated with amino acids catabolism are upregulated in cells following carbohydrate starvation. Collectively, our results support the contention that ETF/ETFQO is an essential pathway to donate electrons to the mETC and that amino acids are alternative substrates to maintain respiration under carbohydrate starvation. © 2017 Scandinavian Plant Physiology Society.

[Effect of different nutritional support on pancreatic secretion in acute pancreatitis].

PubMed

Achkasov, E E; Pugaev, A V; Nabiyeva, Zh G; Kalachev, S V

To develop and justify optimal nutritional support in early phase of acute pancreatitis (AP). 140 AP patients were enrolled. They were divided into groups depending on nutritional support: group I (n=70) - early enteral tube feeding (ETF) with balanced mixtures, group II (n=30) - early ETF with oligopeptide mixture, group III (n=40) - total parenteral nutrition (TPN). The subgroups were also isolated depending on medication: A - Octreotide, B - Quamatel, C - Octreotide + Quamatel. Pancreatic secretion was evaluated by using of course of disease, instrumental methods, APUD-system hormone levels (secretin, cholecystokinin, somatostatin, vasointestinal peptide). ETF was followed by pancreas enlargement despite ongoing therapy, while TPN led to gradual reduction of pancreatic size up to normal values. α-amylase level progressively decreased in all groups, however in patients who underwent ETF (I and II) mean values of the enzyme were significantly higher compared with TPN (group III). Secretin, cholecystokinin and vasointestinal peptide were increasing in most cases, while the level of somatostatin was below normal in all groups. Enteral tube feeding (balanced and oligopeptide mixtures) contributes to pancreatic secretion compared with TPN, but this negative impact is eliminated by antisecretory therapy. Dual medication (Octreotide + Quamatel) is more preferable than monotherapy (Octreotide or Quamatel).

Analysis of semiclassical approximations in the framework of quantumhadrodynamics

NASA Astrophysics Data System (ADS)

Speicher, C.; Engel, E.; Dreizler, R. M.

1993-10-01

We examine the importance of gradient corrections to the Thomas-Fermi (TF) approximation for the description of semi-infinite nuclear matter and nuclei within the framework of quantumhadrodynamics. The discussion of semi-infinite nuclear matter exhibits most clearly how surface properties depend on the parametrisation of the effective interaction. In agreement with earlier studies it is found that for parametrisations with low effective mass the original extended TF (ETF) equations cannot be solved. We present a scheme to overcome this problem which is very much in the spirit of the semiclassical approach and allows for a solution in any situation. For the case of nuclei the results of the TF and ETF approximations are compared to those of Hartree calculations which serve as a standard in this context. In accordance with the sensitivity of semi-infinite nuclear matter results to the specific parametrisation, ETF does not yield a consistent improvement over TF for all parametrisations. However, by examining a larger number of parametrisations it is shown that those cases where TF results are closer to Hartree data have to be regarded as fortuitous and that on average the agreement between ETF and Hartree results is clearly better.

Assignment of Etfdh, Etfb, and Etfa to chromosomes 3, 7, and 13: The mouse homologs of genes respondible for glutaric acidemia type II in human

DOE Office of Scientific and Technical Information (OSTI.GOV)

White, R.A.; Dowler, L.L.; Angeloni, S.V.

Electron transfer flavoprotein (composed of {alpha} and {beta} subunits) is an obligatory electron acceptor for several dehydrogenases and is located in the mitochondrial matrix. Electrons accepted by electron transfer flavo-protein (ETF) are transferred to the main mitochondrial respiratory chain by the way of ETF dehydrogenase (ETFDH). In humans, deficiency of ETF or ETFDH leads to glutaric acidemia type II, an inherited metabolic disorder that can be fatal in its neonatal form and is characterized by severe hypoketotic hypoglycemia and acidosis. We used cDNA probes for the Etfdh, Etfb, and Etfa genes to determine localization of these mouse genes to chromosomesmore » 3, 7, and 13. 18 refs., 3 figs.« less

Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting

NASA Astrophysics Data System (ADS)

Yamahara, Yumi; Nagasawa, Naotsugu; Taguchi, Mitsumasa; Oshima, Akihiro; Washio, Masakazu

2018-01-01

Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (-CF2-CF(OO･)-) on tetrafluoroethylene unit in ETFE.

Low energy ion beam induced changes in ETFE polymer

NASA Astrophysics Data System (ADS)

Parada, M. A.; Delalez, N.; de Almeida, A.; Muntele, C.; Muntele, I.; Ila, D.

2006-01-01

Low energy ion beam bombardment of ethylenetetrafluoroethylene (ETFE) modifies the physical and chemical properties of the polymer surface in ways that enhance or compromise applications in the technological and medical physics fields. When a material is exposed to ionizing radiation, its changes depends on the type, energy and intensity of the applied radiation. In order to determine the nature of the induced radiation changes, ETFE films were bombarded with fluences from 1012 up to 1015 ions/cm2 of keV N and protons. The emission of gaseous species during the bombardments was monitored with a residual gas analyser (RGA). The bombarded films were analysed with optical absorption photospectrometry (OAP), Fourier transform infrared (FTIR) and micro-Raman spectrometries that determine the chemical nature of the structural changes caused by ions bombardment.

Study on depth profile of heavy ion irradiation effects in poly(tetrafluoroethylene-co-ethylene)

NASA Astrophysics Data System (ADS)

Gowa, Tomoko; Shiotsu, Tomoyuki; Urakawa, Tatsuya; Oka, Toshitaka; Murakami, Takeshi; Oshima, Akihiro; Hama, Yoshimasa; Washio, Masakazu

2011-02-01

High linear energy transfer (LET) heavy ion beams were used to irradiate poly(tetrafluoroethylene-co-ethylene) (ETFE) under vacuum and in air. The irradiation effects in ETFE as a function of the depth were precisely evaluated by analyzing each of the films of the irradiated samples, which were made of stacked ETFE films. It was indicated that conjugated double bonds were generated by heavy ion beam irradiation, and their amounts showed the Bragg-curve-like distributions. Also, it was suggested that higher LET beams would induce radical formation in high density and longer conjugated C=C double bonds could be generated by the second-order reactions. Moreover, for samples irradiated in air, C=O was produced correlating to the yield of oxygen molecules diffusing from the sample surface.

Reliable Control Using Disturbance Observer and Equivalent Transfer Function for Position Servo System in Current Feedback Loop Failure

NASA Astrophysics Data System (ADS)

Ishikawa, Kaoru; Nakamura, Taro; Osumi, Hisashi

A reliable control method is proposed for multiple loop control system. After a feedback loop failure, such as case of the sensor break down, the control system becomes unstable and has a big fluctuation even if it has a disturbance observer. To cope with this problem, the proposed method uses an equivalent transfer function (ETF) as active redundancy compensation after the loop failure. The ETF is designed so that it does not change the transfer function of the whole system before and after the loop failure. In this paper, the characteristic of reliable control system that uses an ETF and a disturbance observer is examined by the experiment that uses the DC servo motor for the current feedback loop failure in the position servo system.

Relocation of the Cryo-Test Facility to NASA-MSFC

NASA Technical Reports Server (NTRS)

Sisco, Jimmy D.; McConnaughey, Paul K. (Technical Monitor)

2002-01-01

The Environmental Test Facility (ETF), located at NASA-Marshall Space Flight Center, Huntsville, Alabama, has provided thermal vacuum testing for several major programs since the 1960's. The ETF consists of over 13 thermal vacuum chambers sized and configured to handle the majority of test payloads. Testing is performed around the clock with multiple tests being conducted simultaneously. Chamber selection to achieve the best match with test articles and juggling program schedules, at times, can be a challenge. The ETF's Sunspot chamber has had tests scheduled and operated back-to-back for several years and provides the majority of schedule conflicts. Future test programs have been identified which surpass the current Sunspot availability. This paper describes a very low cost alternate to reduce schedule conflicts by utilizing government excess equipment

Effluent characterization and different modes of reuse in agriculture-a model case study.

PubMed

Das, Madhumita; Kumar, Ashwani

2009-06-01

High-quality waters are steadily retreating worldwide. Discharge of industrial effluent in the environment again declines soil/water quality to a great extent. On the other hand, effluent reuse in agriculture could be a means to conserve natural resources by providing assured water supply for growing crops. But industrial effluents are highly variable in nature, containing a variety of substances, and all are not favorable for farming. Appraisal and developing modes of effluent reuse is therefore a prerequisite to enable its proper use in agriculture. Effluents of various industries were assessed and approaches for their use in farming were developed for a particular region in this study. As per availability of effluents, the same could be implemented in other water-scarce areas. Effluents of 20 different industrial units were characterized by 24 attributes. Comparing these with corresponding irrigation water quality standards, the probability of their reuse was interpreted in the first approach. On the basis of relevant properties of major soil types dominated in a particular region, the soil-based usability of effluent was worked out in the second approach. By emphasizing the limitation of groundwater development where it went beyond 50% exploitation level, the land form and major soil type were then identified by applying a soil-based effluent reuse approach; the area-specific suitability of its use was perceived in the third approach. On the basis of irrigation water quality standards, the irrigation potentials of paper mill, fermentation (breweries and distilleries), and sugar factory effluents were recognized. In a soil-based approach, the compatibility of effluent with soil type was marked with A (preferred) and B (moderately preferred) classes and, compiling their recurring presence, the unanimous preference for paper mill effluent followed by rubber goods manufacturing industries/marine shrimp processing units, fermentation, and sugar mills was noted. Usability of these was also evident from a groundwater exploitation status-based approach. The approaches of assessing industrial effluents differing in compositions systematically reflected the ability and applicability of certain effluents in agriculture. The context-specific assessment of effluent offers options to compare effluent from a range of viewpoints and enhances its reasonability of use for growing crops. Chemical characterization of various industrial effluents first disclosed their potential of reuse. The soil-properties-based compatibility of effluent focused their prospects of use and groundwater-exploitation-status-based portrayed its area of use in a specific region. Assessment of effluent through these enhances reliability and appropriateness of its reuse in agriculture. Options of industrial effluent (prospective) reuse in agriculture provide ways to combat freshwater crisis without degrading environmental quality. It may be applied for assessing effluent before its reuse in several water-starved countries.

Enteral (oral or tube administration) nutritional support and eicosapentaenoic acid in patients with cancer: a systematic review.

PubMed

Elia, M; Van Bokhorst-de van der Schueren, M A E; Garvey, J; Goedhart, A; Lundholm, K; Nitenberg, G; Stratton, R J

2006-01-01

The aim of this systematic review was to determine the efficacy and potential benefits of enteral nutritional support [oral nutritional supplements (ONS) or enteral tube feeding (ETF)], and eicosapentaenoic acid (EPA, free acid, ethyl esters or fish oil; provided as capsules or enriched ONS or ETF) in patients with cancer. Clinical studies were identified using electronic databases, and studies were selected according to predetermined criteria. For each treatment modality (chemo/radiotherapy, surgery, and palliative care), the comparisons of interest were nutritional support vs. routine care (no nutritional support), EPA supplement (capsule or enriched ONS or ETF) vs. routine care (no supplement or standard supplement), ETF vs. parenteral nutrition (PN). The reviewed outcomes were dietary intake, anthropometry, clinical (mortality, length of hospital stay, complications, and quality of life) and haematological/biochemical (white blood cell count, serum transferrin and albumin, CD3-positive lymphocytes, and inflammatory markers). Meta-analyses were performed where possible. In patients undergoing radiotherapy, meta-analysis showed that ONS significantly increase dietary intake (381 kcal/day, 95% CI 193 to 569 in 3 RCTs) compared to routine care. In patients undergoing surgery, meta-analyses showed that ETF results in a significantly shorter length of hospital stay (1.72 fewer days, 95% CI 0.90 to 2.54 in 8 RCTs), lower incidence of any complications (OR 0.62, 95% CI 0.50 to 0.77 in 4 RCTs) and infectious complications (OR 0.67, 95% CI 0.55 to 0.82 in 11 RCTs) and lower sepsis scores (2.21 points, 95% CI 1.49 to 2.92 in 2 RCTs), but no difference in mortality (OR 0.72, 95% CI 0.40 to 1.29 in 7 RCTs) compared to PN. There was also no difference in mortality between ONS or ETF vs. routine care in patients undergoing chemotherapy/radiotherapy (OR 1.00, 95% CI 0.62-1.61 in 4 RCTs) or surgery (OR 2.44, 95% CI 0.75 to 7.95 in 4 RCTs). Individual studies of EPA supplementation as capsules showed improvements in survival, complications and inflammatory markers in patients undergoing bone marrow transplant (BMT). In palliative care patients receiving EPA-enriched ONS or capsules, there were inconsistent positive effects on survival and quality of life. In those undergoing surgery, EPA-enriched ETF had no effect. Further research is required to elucidate the clinical efficacy of enteral nutrition support, including the potential benefits of EPA supplementation, in patients with cancer.

75 FR 48400 - Self-Regulatory Organizations; NASDAQ OMX BX, Inc.; Notice of Filing and Immediate Effectiveness...

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...\\ Id. \\11\\ Id. Under Chapter IV, Section 3(i) of the BOX Rules, Exchange-Traded Fund Shares (``ETFs... traded on BOX: The ETFs must (i) be traded on a national securities exchange; (ii) be defined as an ``NMS... specified non-U.S. currency or currencies deposited with the trust or similar entity when aggregated in some...

76 FR 44969 - Self-Regulatory Organizations; NYSE Arca Inc.; Notice of Filing and Immediate Effectiveness of...

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...[supreg] exchange-traded fund (``SPY ETF''),\\4\\ which list and trade under the option symbol SPY, and to.... The SPDR S&P 500 ETF represents ownership in the SPDR S&P 500 Trust, a unit investment trust that... volume of SPY options). \\7\\ QQQ options were formerly known as options on the Nasdaq-100 Tracking Stock...

75 FR 73145 - Self-Regulatory Organizations; NYSE Arca, Inc.; Notice of Filing of Proposed Rule Change Relating...

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2010-11-29

... roll for each VIX Futures Contract occurs on each index business day according to a pre-determined... and Trading of the Jefferies S&P 500[supreg] VIX Short-Term Futures ETF November 19, 2010. Pursuant to... Short-Term Futures ETF under NYSE Arca Equities Rule 8.200. The text of the proposed rule change is...

Extended characterization of M-Area settling basin and vicinity. Technical data summary. Revision

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pickett, J B

1985-10-01

The Savannah River Plant M-Area settling basin, an unlined surface impoundment, has received process effluents from the M-Area fuel and target fabrication facilities since 1958. The waste effluents have contained metal degreasing agents (chlorinated hydrocarbons), acids, caustics, and heavy metals. Data analyses are provided.

The treatment performance of different subsoils in Ireland receiving on-site wastewater effluent.

PubMed

Gill, L W; O'Súlleabháin, C; Misstear, B D R; Johnston, P J

2007-01-01

Current Irish guidelines require a comprehensive site assessment of a percolation area for wastewater disposal before planning permission is granted for dwellings in rural areas. For a site to be deemed suitable, the subsoil must have a percolation value equivalent to a field saturated hydraulic conductivity in the range 0.08 to 4.2 m d(-1) using a falling head percolation test. A minimum of 1.2 m of unsaturated subsoil must also exist below the invert of the percolation area receiving effluent from a septic tank (or 0.6 m for secondary treated effluent). During a 2-yr period, the three-dimensional performance of four percolation areas treating domestic wastewater was monitored. At each site samples were taken at 0, 10, and 20 m along each of the four percolation trenches at depths of 0.3, 0.6, and 1.0 m below each trench to ascertain the attenuation effects of the unsaturated subsoil. The two sites with septic tanks installed performed at least as well as the other two sites with secondary treatment systems installed and appeared to discharge a better quality effluent in terms of nutrient load. An average of 2.1 and 6.8 g total N d(-1) remained after passing through 1-m depth of subsoil beneath the trenches receiving septic tank effluent compared with 12.7 and 16.7 g total N d(-1) on the sites receiving secondary effluent. The research also indicates that the septic tank effluent was of an equivalent quality to the secondary treated effluent in terms of indicator bacteria (E. coli) after percolating through 0.6-m depth of unsaturated subsoil.

Autologous transplantation versus allogeneic transplantation in patients with follicular lymphoma experiencing early treatment failure.

PubMed

Smith, Sonali M; Godfrey, James; Ahn, Kwang Woo; DiGilio, Alyssa; Ahmed, Sairah; Agrawal, Vaibhav; Bachanova, Veronika; Bacher, Ulrike; Bashey, Asad; Bolaños-Meade, Javier; Cairo, Mitchell; Chen, Andy; Chhabra, Saurabh; Copelan, Edward; Dahi, Parastoo B; Aljurf, Mahmoud; Farooq, Umar; Ganguly, Siddhartha; Hertzberg, Mark; Holmberg, Leona; Inwards, David; Kanate, Abraham S; Karmali, Reem; Kenkre, Vaishalee P; Kharfan-Dabaja, Mohamed A; Klein, Andreas; Lazarus, Hillard M; Mei, Matthew; Mussetti, Alberto; Nishihori, Taiga; Ramakrishnan Geethakumari, Praveen; Saad, Ayman; Savani, Bipin N; Schouten, Harry C; Shah, Nirav; Urbano-Ispizua, Alvaro; Vij, Ravi; Vose, Julie; Sureda, Anna; Hamadani, Mehdi

2018-06-15

Early treatment failure (ETF) in follicular lymphoma (FL), defined as relapse or progression within 2 years of frontline chemoimmunotherapy, is a newly recognized marker of poor survival and identifies a high-risk group of patients with an expected 5-year overall survival (OS) rate of approximately 50%. Transplantation is an established option for relapsed FL, but its efficacy in this specific ETF FL population has not been previously evaluated. This study compared autologous hematopoietic stem cell transplantation (auto-HCT) with either matched sibling donor (MSD) or matched unrelated donor (MUD) allogeneic hematopoietic cell transplantation (allo-HCT) as the first transplantation approach for patients with ETF FL (age ≥ 18 years) undergoing auto-HCT or allo-HCT between 2002 and 2014. The primary endpoint was OS. The secondary endpoints were progression-free survival, relapse, and nonrelapse mortality (NRM). Four hundred forty FL patients had ETF (auto-HCT, 240; MSD hematopoietic stem cell transplantation [HCT], 105; and MUD HCT, 95). With a median follow-up of 69 to 73 months, the adjusted probability of 5-year OS was significantly higher after auto-HCT (70%) or MSD HCT (73%) versus MUD HCT (49%; P = .0008). The 5-year adjusted probability of NRM was significantly lower for auto-HCT (5%) versus MSD (17%) or MUD HCT (33%; P < .0001). The 5-year adjusted probability of disease relapse was lower with MSD (31%) or MUD HCT (23%) versus auto-HCT (58%; P < .0001). Patients with high-risk FL, as defined by ETF, undergoing auto-HCT for FL have low NRM and a promising 5-year OS rate (70%). MSD HCT has lower relapse rates than auto-HCT but similar OS. Cancer 2018;124:2541-51. © 2018 American Cancer Society. © 2018 American Cancer Society.

Oxidation of the FAD cofactor to the 8-formyl-derivative in human electron-transferring flavoprotein

PubMed Central

Augustin, Peter; Toplak, Marina; Fuchs, Katharina; Gerstmann, Eva Christine; Prassl, Ruth; Winkler, Andreas; Macheroux, Peter

2018-01-01

The heterodimeric human (h) electron-transferring flavoprotein (ETF) transfers electrons from at least 13 different flavin dehydrogenases to the mitochondrial respiratory chain through a non-covalently bound FAD cofactor. Here, we describe the discovery of an irreversible and pH-dependent oxidation of the 8α-methyl group to 8-formyl-FAD (8f-FAD), which represents a unique chemical modification of a flavin cofactor in the human flavoproteome. Furthermore, a set of hETF variants revealed that several conserved amino acid residues in the FAD-binding pocket of electron-transferring flavoproteins are required for the conversion to the formyl group. Two of the variants generated in our study, namely αR249C and αT266M, cause glutaric aciduria type II, a severe inherited disease. Both of the variants showed impaired formation of 8f-FAD shedding new light on the potential molecular cause of disease development. Interestingly, the conversion of FAD to 8f-FAD yields a very stable flavin semiquinone that exhibited slightly lower rates of electron transfer in an artificial assay system than hETF containing FAD. In contrast, the formation of 8f-FAD enhanced the affinity to human dimethylglycine dehydrogenase 5-fold, indicating that formation of 8f-FAD modulates the interaction of hETF with client enzymes in the mitochondrial matrix. Thus, we hypothesize that the FAD cofactor bound to hETF is subject to oxidation in the alkaline (pH 8) environment of the mitochondrial matrix, which may modulate electron transport between client dehydrogenases and the respiratory chain. This discovery challenges the current concepts of electron transfer processes in mitochondria. PMID:29301933

Microstructural characterization of ultra thin copper interconnects

NASA Astrophysics Data System (ADS)

Yang, Hee-Dong

The present study investigates the defects related to reliability issues, such as physical failures developed during processing and end use. In the first part of this study, kinetic analysis using the Johnson-Mehl-Avrami (JMA) model demonstrates that a self-annealing mechanism in electroplated Cu films depends on the film properties, such as thickness and the amount of crystal defects in an as-deposited state. In order to obtain the evidence of such defects, the microstructural characterization of defects in ultra thin copper interconnects using transmission electron microscopy (TEM) is presented. Examination of the defects using TEM reveals that voids filled with gas form as a lens shape along the {110} habit planes of the copper matrix. In the second part of this study, methodology and results of an electro-thermal-fatigue (ETF) testing, designed for early detection of process defects, are presented. Such ETF testing combines high-density current electrical stressing and thermal cycling to accelerate the evolution of defects in Cu interconnects. In ETF testing, the evolution of defects provides the nucleation sites for voids which open or close during thermal cycling. Then, the accumulation of voids creates the change in resistance when they reach a critical size. As a result of voids evolution, the high current density and high joule heating create a transient resistance increase. ETF testing reveals two failure modes, and the mode-I failure has the importance in detecting defects. The number of cycles to failure in ETF testing decreases with higher current density, but the rate of thermal cycling has no effect. Results from this investigation suggest that impurities in the copper electrodeposition process must be carefully controlled to achieve reliable ultra thin copper interconnects.

Dose versus diagnosis: iatrogenic radiation exposure by multidetector computerised tomography in an academic emergency department with measurement of clinically actionable results and emergently treatable findings.

PubMed

Baskerville, J R; Chang, J H; Viator, M; Rutledge, W; Miryala, R; Duval, K E; Nishino, T K

2009-01-01

To determine the iatrogenic absorbed dosage of radiation of the patient in milligray (mGy) computerised tomography dose index volume (CTDIvol) when tested with multidetector computerised tomography (MDCT) in the emergency department (ED) setting and calculate the absorbed dosage of radiation per clinically actionable result and emergently treatable finding (ETF). The University of Texas Medical Branch (UTMB) ED located in Galveston, Texas, USA, is a level 1 trauma and tertiary referral centre treating 70,000 patients per annum. A retrospective cross-sectional data analysis of 770 emergency patients investigated by MDCT in July 2007. The presence of actionable results and ETF were determined by chart review. A total of 5320 emergency patients was treated in the UTMB ED in July 2007. This included 4508 medical and 812 trauma patients. A total of 1094 MDCT studies was performed, of which complete data were available on 1046. A total of 770 patients was investigated by MDCT, representing 14.47% of all emergency patients. This included 33.99% of trauma patients and 10.96% of medical patients. Actionable results were found in 341 studies and ETF in 105 studies. The mean radiation was 163.27 and 530.23 mGy CTDIvol for actionable results and ETF, respectively, for all studies. The mean radiation was 53.27 and 106.36 mGy CTDIvol for medical and trauma patients, respectively. The absorbed dosage of radiation of patients investigated by MDCT is clinically significant. The actionable results and ETF in our study demonstrate considerable opportunity for improvement in the utilisation of this technology by physicians.

Three-dimensional structure of human electron transfer flavoprotein to 2.1-Å resolution

PubMed Central

Roberts, David L.; Frerman, Frank E.; Kim, Jung-Ja P.

1996-01-01

Mammalian electron transfer flavoproteins (ETF) are heterodimers containing a single equivalent of flavin adenine dinucleotide (FAD). They function as electron shuttles between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The structure of human ETF solved to 2.1-Å resolution reveals that the ETF molecule is comprised of three distinct domains: two domains are contributed by the α subunit and the third domain is made up entirely by the β subunit. The N-terminal portion of the α subunit and the majority of the β subunit have identical polypeptide folds, in the absence of any sequence homology. FAD lies in a cleft between the two subunits, with most of the FAD molecule residing in the C-terminal portion of the α subunit. Alignment of all the known sequences for the ETF α subunits together with the putative FixB gene product shows that the residues directly involved in FAD binding are conserved. A hydrogen bond is formed between the N5 of the FAD isoalloxazine ring and the hydroxyl side chain of αT266, suggesting why the pathogenic mutation, αT266M, affects ETF activity in patients with glutaric acidemia type II. Hydrogen bonds between the 4′-hydroxyl of the ribityl chain of FAD and N1 of the isoalloxazine ring, and between αH286 and the C2-carbonyl oxygen of the isoalloxazine ring, may play a role in the stabilization of the anionic semiquinone. With the known structure of medium chain acyl-CoA dehydrogenase, we hypothesize a possible structure for docking the two proteins. PMID:8962055

Post-treatment of UASB reactor effluent in waste stabilization ponds and in horizontal flow constructed wetlands: a comparative study in pilot scale in Southeast Brazil.

PubMed

Bastos, R K X; Calijuri, M L; Bevilacqua, P D; Rios, E N; Dias, E H O; Capelete, B C; Magalhães, T B

2010-01-01

The results of a 20-month period study in Brazil were analyzed to compare horizontal-flow constructed wetlands (CW) and waste stabilization pond (WSP) systems in terms of land area requirements and performance to produce effluent qualities for surface water discharge, and for wastewater use in agriculture and/or aquaculture. Nitrogen, E. coli and helminth eggs were more effectively removed in WSP than in CW. It is indicated that CW and WSP require similar land areas to achieve a bacteriological effluent quality suitable for unrestricted irrigation (10(3) E. coli per 100 mL), but CW would require 2.6 times more land area than ponds to achieve quite relaxed ammonia effluent discharge standards (20 mg NH(3) L(-1)), and, by far, more land than WSP to produce an effluent complying with the WHO helminth guideline for agricultural use (< or =1 egg per litre).

Impact of uniform electrode current distribution on ETF. [Engineering Test Facility MHD generator

NASA Technical Reports Server (NTRS)

Bents, D. J.

1982-01-01

A basic reason for the complexity and sheer volume of electrode consolidation hardware in the MHD ETF Powertrain system is the channel electrode current distribution, which is non-uniform. If the channel design is altered to provide uniform electrode current distribution, the amount of hardware required decreases considerably, but at the possible expense of degraded channel performance. This paper explains the design impacts on the ETF electrode consolidation network associated with uniform channel electrode current distribution, and presents the alternate consolidation designs which occur. They are compared to the baseline (non-uniform current) design with respect to performance, and hardware requirements. A rational basis is presented for comparing the requirements for the different designs and the savings that result from uniform current distribution. Performance and cost impacts upon the combined cycle plant are discussed.

The adenovirus oncoprotein E1a stimulates binding of transcription factor ETF to transcriptionally activate the p53 gene.

PubMed

Hale, T K; Braithwaite, A W

1999-08-20

Expression of the tumor suppressor protein p53 plays an important role in regulating the cellular response to DNA damage. During adenovirus infection, levels of p53 protein also increase. It has been shown that this increase is due not only to increased stability of the p53 protein but to the transcriptional activation of the p53 gene during infection. We demonstrate here that the E1a proteins of adenovirus are responsible for activating the mouse p53 gene and that both major E1a proteins, 243R and 289R, are required for complete activation. E1a brings about the binding of two cellular transcription factors to the mouse p53 promoter. One of these, ETF, binds to three upstream sites in the p53 promoter and one downstream site, whereas E2F binds to one upstream site in the presence of E1a. Our studies indicate that E2F binding is not essential for activation of the p53 promoter but that ETF is. Our data indicate the ETF site located downstream of the start site of transcription is the key site in conferring E1a responsiveness on the p53 promoter.

Impediments to predicting site response: Seismic property estimation and modeling simplifications

USGS Publications Warehouse

Thompson, E.M.; Baise, L.G.; Kayen, R.E.; Guzina, B.B.

2009-01-01

We compare estimates of the empirical transfer function (ETF) to the plane SH-wave theoretical transfer function (TTF) within a laterally constant medium for invasive and noninvasive estimates of the seismic shear-wave slownesses at 13 Kiban-Kyoshin network stations throughout Japan. The difference between the ETF and either of the TTFs is substantially larger than the difference between the two TTFs computed from different estimates of the seismic properties. We show that the plane SH-wave TTF through a laterally homogeneous medium at vertical incidence inadequately models observed amplifications at most sites for both slowness estimates, obtained via downhole measurements and the spectral analysis of surface waves. Strategies to improve the predictions can be separated into two broad categories: improving the measurement of soil properties and improving the theory that maps the 1D soil profile onto spectral amplification. Using an example site where the 1D plane SH-wave formulation poorly predicts the ETF, we find a more satisfactory fit to the ETF by modeling the full wavefield and incorporating spatially correlated variability of the seismic properties. We conclude that our ability to model the observed site response transfer function is limited largely by the assumptions of the theoretical formulation rather than the uncertainty of the soil property estimates.

Transforming to a Cost-Management Culture

DTIC Science & Technology

2009-02-02

Force ( ETF ). Their challenge “is to adapt our institutions to 13 cement the transformation of the Army of the 21st Century.”34 LTG Durbin is the...responsible for the oversight of the ETF .35 These types of leadership involvement and directives, along with supporting tools, are necessary to...document shared with AAMC CGEA Faculty Development SIG, March, 2001. Contact lroth@med.wayne.edu. 16 Geiger, Winning the Cost War, 1. 17 David Gold

The Reform of Vocational Curricula: Outcomes of the 2005 ETF Peer Reviews in Bulgaria, Bosnia and Herzegovina, the Former Yugoslav Republic of Macedonia, and Serbia

ERIC Educational Resources Information Center

Parkes, David; Nielsen, Soren

2006-01-01

The European Training Foundation EU European (ETF) Peer Review is an initiative that was launched in the South Eastern European countries in 2002. It combines policy advice to individual countries with a regional dimension. The key objectives for the peer reviews are to: (1) provide an external assessment of VET (vocational education and training)…

Development of a Plastic Rotating Band for High Performance Projectiles

DTIC Science & Technology

1974-07-01

changes in the program: a. It became clearly unfeasible to include a significant mechanical anchorage in that restricted depth. b. The stock of TP...Wilmington, Delaware responded. Tefzel is a ETFE material, or a copolymer of TFE (Teflon) with ethylene. The properties and merits of Tefzel for this...polycarbonate was discontinued. Valox 420 with 30 percent glass failed at -650F. Tefzel ETFE fluoropolymer unfilled and with 25 percent glass was tried; the

DOE Office of Scientific and Technical Information (OSTI.GOV)

Antonacci, R.; Colombo, I.; Volta, M.

The electron-transfer flavoprotein (ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain electrons by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. ETF is composed of a 35-kDa [alpha]-subunit that is cleaved to a 32-kDa protein during mitochondrial import (ETFA) and a [beta]-subunit that reaches the mitochondrion unmodified (ETFB). The cDNA encoding both these subunits has been cloned and sequenced. 14 refs., 1 fig.

Impact of uniform electrode current distribution on ETF

NASA Technical Reports Server (NTRS)

Bents, D. J.

1982-01-01

The design impacts on the ETF electrode consolidation network associated with uniform channel electrode current distribution are examined and the alternate consolidation design which occur are presented compared to the baseline (non-uniform current) design with respect to performance, and hardware requirements. A rational basis is given for comparing the requirements for the different designs and the savings that result from uniform current distribution. Performance and cost impacts upon the combined cycle plant are discussed.

Effluent quality from 200 on-site sewage systems: design values for guidelines.

PubMed

Charles, K J; Ashbolt, N J; Roser, D J; McGuinness, R; Deere, D A

2005-01-01

The quality of effluent from an on-site sewage treatment system is a critical factor in designing the disposal area and, hence, ensuring the sustained performance of the system. Contaminant concentrations in effluent are typically specified in regulatory guidelines or standards; however, the accuracy of these guideline values are brought into question due to the poor performance of septic tanks and the high failure rates of disposal systems reported here and elsewhere. Results from studies of septic tank effluent quality indicated that the effluent is of poorer quality than currently suggested by guidelines. Aerated wastewater treatment systems were found to perform to accreditation guidelines; however, insufficient nutrient data is presently available to assess nutrient loads. It is proposed that the 80th percentile of system performance be adopted as the design value for sizing effluent disposal areas to minimise failure associated with overloading. For septic tanks this equates to 660 mg L(-1) SS, 330 mg L(-1) BOD, 250 mg L(-1) TN and 36 mg L(-1) TP.

ETF system code: composition and applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reid, R.L.; Wu, K.F.

1980-01-01

A computer code has been developed for application to ETF tokamak system and conceptual design studies. The code determines cost, performance, configuration, and technology requirements as a function of tokamak parameters. The ETF code is structured in a modular fashion in order to allow independent modeling of each major tokamak component. The primary benefit of modularization is that it allows updating of a component module, such as the TF coil module, without disturbing the remainder of the system code as long as the input/output to the modules remains unchanged. The modules may be run independently to perform specific design studies,more » such as determining the effect of allowable strain on TF coil structural requirements, or the modules may be executed together as a system to determine global effects, such as defining the impact of aspect ratio on the entire tokamak system.« less

An empirical study into the effect of long term storage (-36±2 °C) of electron-beamed ETFE on the properties of radiation-grafted alkaline anion-exchange membranes

NASA Astrophysics Data System (ADS)

Kizewski, Jamie Peter; Mudri, Nurul H.; Varcoe, John R.

2013-08-01

The application of alkaline anion-exchange membranes (AAEM) in solid alkaline fuel cells is growing in prominence mainly due to enhanced tolerance to carbon dioxide, compared to alkaline fuel cells containing aqueous electrolytes, and the potential for using non precious metal catalysts. Radiation grafting is a common methodology for the production of functional polymers and membranes. This statistical study examines the synthesis of radiation grafted AAEMs that are formed from electron beam irradiated poly(ethylene-co-tetrafluoroethylene), EB-ETFE. It is shown that EB-ETFE can be cold stored for at least 16 months and still be used to produce ionically conductive AAEMs. The limitations of routine measurements of properties, such as dimensional increases, ion-exchange capacity, water uptakes and ionic conductivities, are also highlighted.

Formation of high mass carbon cluster ions from laser ablation of polymers and thin carbon films

NASA Astrophysics Data System (ADS)

Creasy, William R.; Brenna, J. T.

1990-02-01

Three materials were studied by laser ablation/Fourier transform mass spectrometry, using 266 nm laser radiation: a copolymer of ethylene and tetrafluoroethylene (ETFE), polyphenylene sulfide (PPS), and a diamond-like carbon film (DLC). In each case, positive ion mass spectra exhibit primarily even-numbered, high mass carbon clusters (``fullerenes'') of the type previously reported for graphite ablation. In the case of ETFE, a large C+60 peak (``buckminsterfullerene'') was observed. The polymer spectra showed a strong dependence on the number of laser pulses on one spot and the laser power density. For ETFE, the fullerene ion relative intensity first increases and then decreases as a function of the number of laser pulses. For the DLC film, fullerenes are observed with a single laser pulse on a fresh spot of the sample. The results are interpreted in terms of a gas phase growth model for the fullerene ion formation.

Study of the surface activation of ETFE by low energy (keV) Si and N bombardment

NASA Astrophysics Data System (ADS)

Parada, M. A.; de Almeida, A.; Muntele, C.; Muntele, I.; Delalez, N.; Ila, D.

2005-12-01

The ethylenetetrafluoroethylene (ETFE) is a polymer formed by alternating ethylene and tetrafluoroethylene segments. It can be applied in the field of medical physics as intra venous catheters and as radiation dosimeters. The increasing application of polymeric materials in technological and scientific fields has motivated the use of surface treatments to modify the physical and chemical properties of polymer surfaces. When a material is exposed to ionizing radiation, it suffers damage leading to surface activation depending on the type, energy and intensity of the applied radiation. In order to determine the radiation damage and the surface activation mechanism ETFE films were bombarded with keV Si and N at various fluences. The bombarded film was also analyzed with optical absorption photospectrometry (OAP), Raman and Fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy showing quantitatively the chemical nature at the damage caused by the Si and N bombardment.

Radiation-grafted proton exchange membranes based on co-grafting from binary monomer mixtures into poly(ethylene-co-tetrafluoroethylene) (ETFE) film

NASA Astrophysics Data System (ADS)

Sohn, Joon-Yong; Sung, Hae-Jun; Song, Joo-Myung; Shin, Junhwa; Nho, Young-Chang

2012-08-01

In this study, proton exchange membranes (PEMs) based on a poly(ethylene-co-tetrafluoroethylene) (ETFE) film were synthesized through the graft copolymerization of styrene and VTMS (vinyltrimethoxysilane), or styrene and TMSPM (3-(trimethoxysilyl) propyl methacrylate) binary monomer systems using a simultaneous irradiation method. The prepared membranes with the similar degrees of grafting were investigated by measuring ion exchange capacity, proton conductivity, water uptake, chemical stability, and dimensional stability. The results indicate that the silane-crosslinked proton exchange membrane (PEM) has not only lower water uptake and dimensional change but also high proton conductivity at low humidity condition compared to non-crosslinked poly(ethylene-co-tetrafluoroethylene)-g-poly(styrene sulfonic acid) (ETFE-g-PSSA). Also, the chemical stability of silane-crosslinked fuel cell membranes was more improved than that of non-crosslinked fuel cell membrane.

Analytical mass formula and nuclear surface properties in the ETF approximation. Part II: asymmetric nuclei

NASA Astrophysics Data System (ADS)

Aymard, François; Gulminelli, Francesca; Margueron, Jérôme

2016-08-01

We have recently addressed the problem of the determination of the nuclear surface energy for symmetric nuclei in the framework of the extended Thomas-Fermi (ETF) approximation using Skyrme functionals. We presently extend this formalism to the case of asymmetric nuclei and the question of the surface symmetry energy. We propose an approximate expression for the diffuseness and the surface energy. These quantities are analytically related to the parameters of the energy functional. In particular, the influence of the different equation of state parameters can be explicitly quantified. Detailed analyses of the different energy components (local/non-local, isoscalar/isovector, surface/curvature and higher order) are also performed. Our analytical solution of the ETF integral improves previous models and leads to a precision of better than 200 keV per nucleon in the determination of the nuclear binding energy for dripline nuclei.

Bacterial challenge of NISSHO ultrafilter ETF 609: results of in vitro testing.

PubMed

Krautzig, S; Lonnemann, G; Shaldon, S; Koch, K M

1996-07-01

In hemodialysis, a certain degree of bacterial contamination on the dialysate side is a regular finding. Concern has been growing that this contamination may lead to a chronic inflammatory response in the patient. Ultrafiltration of dialysate can be used to reduce bacterial content and levels of cytokine-inducing substances upstream of the patient's dialyzer. The aim of this study was to test in vitro the rejection capacity of a polysulfone hollow-fiber ultrafilter (ETF 609, NISSHO Co., Osaka, Japan) challenged with bacterial filtrates derived from Pseudomonas aeruginosa PA103. Results showed a reduction of interleukin-1 beta-inducing activity (measured on peripheral blood mononuclear cells) from 5,035 +/- 394 pg/ml prefilter to nondetectable levels postfilter and endotoxin levels (limulus amebocyte lysate assay) of 4,167 +/- 1,079 versus 12 +/- 2 pg/ml, respectively. In conclusion, ultrafiltration of dialysate with the polysulfone ultrafilter ETF 609 leads to a potent reduction of cytokine-inducing activity.

Investigations in site response from ground motion observations in vertical arrays

NASA Astrophysics Data System (ADS)

Baise, Laurie Gaskins

The aim of the research is to improve the understanding of earthquake site response and to improve the techniques available to investigate issues in this field. Vertical array ground motion data paired with the empirical transfer function (ETF) methodology is shown to accurately characterize site response. This manuscript draws on methods developed in the field of signal processing and statistical time series analysis to parameterize the ETF as an autoregressive moving-average (ARMA) system which is justified theoretically, historically, and by example. Site response is evaluated at six sites in California, Japan, and Taiwan using ETF estimates, correlation analysis, and full waveform modeling. Correlation analysis is proposed as a required data quality evaluation imperative to any subsequent site response analysis. ETF estimates and waveform modeling are used to decipher the site response at sites with simple and complex geologic structure, which provide simple time-invariant and time-variant methods for evaluating both linear site transfer functions and nonlinear site response for sites experiencing liquefaction of the soils. The Treasure and Yerba Buena Island sites, however, require 2-D waveform modeling to accurately evaluate the effects of the shallow sedimentary basin. ETFs are used to characterize the Port Island site and corresponding shake table tests before, during, and after liquefaction. ETFs derived from the shake table tests were demonstrated to consistently predict the linear field ground response below 16 m depth and the liquefied behavior above 15 m depth. The liquefied interval response was demonstrated to gradually return to pre-liquefied conditions within several weeks of the 1995 Hyogo-ken Nanbu earthquake. Both the site's and the shake table test's response were shown to be effectively linear up to 0.5 g in the native materials below 16 m depth. The effective linearity of the site response at GVDA, Chiba, and Lotting up to 0.1 g, 0.33 g, and 0.49 g, respectively, further confirms that site response in the field may be more linear than expected from laboratory tests. Strong motions were predicted at these sites with normalized mean square error less than 0.10 using ETFs generated from weak motions. The Treasure Island site response was shown to be dominated by surface waves propagating in the shallow sediments of the San Francisco Bay. Low correlation of the ground motions recorded on rock at Yerba Buena Island and in rock beneath the Treasure Island site intimates that the Yerba Buena site is an inappropriate reference site for Treasure Island site response studies. Accurate simulation of the Treasure Island site response was achieved using a 2-D velocity structure comprised of a 100 m uniform soil basin (Vs = 400 m/s) over a weathered rock veneer (Vs = 1.5 km/s) to 200 m depth.

300 Area treated effluent disposal facility sampling schedule

DOE Office of Scientific and Technical Information (OSTI.GOV)

Loll, C.M.

1994-10-11

This document is the interface between the 300 Area Liquid Effluent Process Engineering (LEPE) group and the Waste Sampling and Characterization Facility (WSCF), concerning process control samples. It contains a schedule for process control samples at the 300 Area TEDF which describes the parameters to be measured, the frequency of sampling and analysis, the sampling point, and the purpose for each parameter.

Radiation-induced phenomena in ethylene-co-tetrafluoroethylene polymer. Temperature and LET effects

NASA Astrophysics Data System (ADS)

Oshima, Akihiro; Washio, Masakazu

2003-08-01

Irradiation temperature and linear energy transfer (LET) dependency on radiation-induced reactions of ethylene-co-tetrafluoroethylene polymer (ETFE) were investigated precisely by using low and high LET beams, and in a wide range of irradiation temperatures from 77 to 573 K including its melting temperature, respectively. At various temperatures irradiation by low LET beam such as γ-rays or electron beams, significant changes were observed in the photo-absorption spectra in the wavelength region between 200 and 500 nm. The general tendency is that the absorption band shifts to longer wavelengths with higher irradiation temperatures. The enhancement of the photo-absorption at 200-500 nm is due to the formation of conjugated double bonds in ETFE by irradiation. By high LET beam irradiation at room temperature such as ion beams, the photo-absorption spectra was different from those of low LET beams, i.e. the new absorption bands around 250-450 nm was appeared. It could be suggested that the high LET beams induced the production of intermediate species in a localized area such as track structure. As a result, reaction kinetics are different from low LET beams.

Effects of spray-irrigated treated effluent on water quantity and quality, and the fate and transport of nitrogen in a small watershed, New Garden Township, Chester County, Pennsylvania

USGS Publications Warehouse

Schreffler, Curtis L.; Galeone, Daniel G.; Veneziale, John M.; Olson, Leif E.; O'Brien, David L.

2005-01-01

An increasing number of communities in Pennsylvania are implementing land-treatment systems to dispose of treated sewage effluent. Disposal of treated effluent by spraying onto the land surface, instead of discharging to streams, may recharge the ground-water system and reduce degradation of stream-water quality. The U.S. Geological Survey (USGS), in cooperation with the Pennsylvania Department of Environmental Protection (PaDEP) and the Chester County Water Resources Authority (CCWRA) and with assistance from the New Garden Township Sewer Authority, conducted a study from October 1997 through December 2001 to assess the effects of spray irrigation of secondary treated sewage effluent on the water quantity and quality and the fate and transport of nitrogen in a 38-acre watershed in New Garden Township, Chester County, Pa. On an annual basis, the spray irrigation increased the recharge to the watershed. Compared to the annual recharge determined for the Red Clay Creek watershed above the USGS streamflow-gaging station (01479820) near Kennett Square, Pa., the spray irrigation increased annual recharge in the study watershed by approximately 8.8 in. (inches) in 2000 and 4.3 in. in 2001. For 2000 and 2001, the spray irrigation increased recharge 65-70 percent more than the recharge estimates determined for the Red Clay Creek watershed. The increased recharge was equal to 30-39 percent of the applied effluent. The spray-irrigated effluent increased base flow in the watershed. The magnitude of the increase appeared to be related to the time of year when the application rates increased. During the late fall through winter and into the early spring period, when application rates were low, base flow increased by approximately 50 percent over the period prior to effluent application. During the early spring through summer to the late fall period, when application rates were high, base flow increased by approximately 200 percent over the period prior to effluent application. The spray-irrigated effluent affected the ground-water quality of the shallow aquifer differently on the hilltop and hillside topographic settings of the watershed where spray irrigation was being applied (application area). Concentrations of nitrate-nitrogen (nitrate N) and chloride (Cl) in the effluent were higher than concentrations of these constituents in shallow ground water from wells on the hilltop and hillside prior to start of spray irrigation. In water from wells on the hilltop, concentrations of nitrate N and Cl increased in samples collected during effluent application compared to samples collected prior to effluent application. Also, increasing trends in concentration of these two constituents were evident through the study period. In water from wells on the hillside, which were on the eastern part of the application area, nitrate N and Cl concentrations increased in samples collected during effluent application compared to samples collected prior to effluent application. Also, increasing trends in concentration of these two constituents were evident through the study period. However, on the hillside of the western application area, the ground-water quality was not affected by the spray-irrigated effluent because of the greater thickness of unconsolidated material and higher amounts of clay present in those unconsolidated sands. Although nitrate N concentrations increased in water from hilltop and hillside wells in the application area, the nitrate N concentrations were below the effluent concentration. A combination of plant uptake, biological activity, and denitrification may be the processes accounting for the lower nitrate N concentrations in shallow ground water compared to the spray-irrigated effluent. Cl concentrations in water from hilltop western application area well Ch-5173 increased during the study period but were an order of magnitude less than the input effluent concentration. Cl concentrations in shallow ground water in the e

40 CFR 434.53 - Effluent limitations guidelines representing the degree of effluent reduction attainable by...

Code of Federal Regulations, 2013 CFR

2013-07-01

... economically achievable (BAT). 434.53 Section 434.53 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS (CONTINUED) COAL MINING POINT SOURCE CATEGORY BPT, BAT, BCT... economically achievable (BAT). (a) Reclamation areas. The limitations of this subsection apply to discharges...

Detailing the equivalence between real equiangular tight frames and certain strongly regular graphs

NASA Astrophysics Data System (ADS)

Fickus, Matthew; Watson, Cody E.

2015-08-01

An equiangular tight frame (ETF) is a set of unit vectors whose coherence achieves the Welch bound, and so is as incoherent as possible. They arise in numerous applications. It is well known that real ETFs are equivalent to a certain subclass of strongly regular graphs. In this note, we give some alternative techniques for understanding this equivalence. In a later document, we will use these techniques to further generalize this theory.

Highly conductive and transparent thin ZnO films prepared in situ in a low pressure system

NASA Astrophysics Data System (ADS)

Ataev, B. M.; Bagamadova, A. M.; Mamedov, V. V.; Omaev, A. K.; Rabadanov, M. R.

1999-03-01

Sucessful preparation of ZnO : M epitaxial thin films (ETF) in situ doped with donor impurity M=Ga, Sn by chemical vapor despsition in a low-pressure system is reported. Highly conductive (up to 10 -4 Ω cm) and transparent ( T>85%) ZnO : M ETF have been successfully produced on single crystal (1012) sapphire substrates. Electrical properties of the films as well as their excition luminescence were studied.

Trigger design for a gamma ray detector of HIRFL-ETF

NASA Astrophysics Data System (ADS)

Du, Zhong-Wei; Su, Hong; Qian, Yi; Kong, Jie

2013-10-01

The Gamma Ray Array Detector (GRAD) is one subsystem of HIRFL-ETF (the External Target Facility (ETF) of the Heavy Ion Research Facility in Lanzhou (HIRFL)). It is capable of measuring the energy of gamma-rays with 1024 CsI scintillators in in-beam nuclear experiments. The GRAD trigger should select the valid events and reject the data from the scintillators which are not hit by the gamma-ray. The GRAD trigger has been developed based on the Field Programmable Gate Array (FPGAs) and PXI interface. It makes prompt trigger decisions to select valid events by processing the hit signals from the 1024 CsI scintillators. According to the physical requirements, the GRAD trigger module supplies 12-bit trigger information for the global trigger system of ETF and supplies a trigger signal for data acquisition (DAQ) system of GRAD. In addition, the GRAD trigger generates trigger data that are packed and transmitted to the host computer via PXI bus to be saved for off-line analysis. The trigger processing is implemented in the front-end electronics of GRAD and one FPGA of the GRAD trigger module. The logic of PXI transmission and reconfiguration is implemented in another FPGA of the GRAD trigger module. During the gamma-ray experiments, the GRAD trigger performs reliably and efficiently. The function of GRAD trigger is capable of satisfying the physical requirements.

Role of METTL20 in regulating β-oxidation and heat production in mice under fasting or ketogenic conditions.

PubMed

Shimazu, Tadahiro; Furuse, Tamio; Balan, Shabeesh; Yamada, Ikuko; Okuno, Shuzo; Iwanari, Hiroko; Suzuki, Takehiro; Hamakubo, Takao; Dohmae, Naoshi; Yoshikawa, Takeo; Wakana, Shigeharu; Shinkai, Yoichi

2018-01-19

METTL20 is a seven-β-strand methyltransferase that is localised to the mitochondria and tri-methylates the electron transfer flavoprotein (ETF) β subunit (ETFB) at lysines 200 and 203. It has been shown that METTL20 decreases the ability of ETF to extract electrons from medium-chain acyl-coenzyme A (CoA) dehydrogenase (MCAD) and glutaryl-CoA dehydrogenase in vitro. METTL20-mediated methylation of ETFB influences the oxygen consumption rate in permeabilised mitochondria, suggesting that METTL20-mediated ETFB methylation may also play a regulatory role in mitochondrial metabolism. In this study, we generated Mettl20 knockout (KO) mice to uncover the in vivo functions of METTL20. The KO mice were viable, and a loss of ETFB methylation was confirmed. In vitro enzymatic assays revealed that mitochondrial ETF activity was higher in the KO mice than in wild-type mice, suggesting that the KO mice had higher β-oxidation capacity. Calorimetric analysis showed that the KO mice fed a ketogenic diet had higher oxygen consumption and heat production. A subsequent cold tolerance test conducted after 24 h of fasting indicated that the KO mice had a better ability to maintain their body temperature in cold environments. Thus, METTL20 regulates ETF activity and heat production through lysine methylation when β-oxidation is highly activated.

Prevalence, Outcomes, and Management of Enteral Tube Feeding Intolerance: A Retrospective Cohort Study in a Tertiary Center.

PubMed

Wang, Kailun; McIlroy, Kerry; Plank, Lindsay D; Petrov, Max S; Windsor, John A

2017-08-01

Enteral tube feeding (ETF) is the most common form of artificial feeding in hospitalized patients, and the development of intolerance (ETFI) is the most common complication. This study aimed to determine the prevalence of ETFI, the clinical consequences, and the current management approach to ETFI in hospitalized adult patients. Adult patients receiving ETF were identified from a prospective database in the Nutrition Services at Auckland City Hospital. Further information was obtained by the review of clinical records for a 12-month period, up to December 2014. The prevalence of ETFI was 33% among 754 patients. ETFI more frequently occurred in the intensive care unit ( P < .05). Patients with ETFI were less likely to reach their feeding goal rate ( P < .01). Multivariate analysis showed that younger age, certain specialties, and acute mesenteric ischemia were independent predictors of ETFI ( P < .05). The management of ETFI was highly variable. Medication was the most common treatment, while changes in the feeding protocol such as reducing infusion rate and stopping and changing the route of ETF were also frequently attempted. ETFI is a frequent problem in adult hospitalized patients receiving ETF, and it is associated with poor clinical outcomes such as inadequate nutrition and complications of feeding. While the pathophysiology is poorly understood, there also appears to be no standard evidence-based treatment. Studies investigating the mechanisms and optimized management are therefore indicated.

300 area TEDF NPDES Permit Compliance Monitoring Plan

DOE Office of Scientific and Technical Information (OSTI.GOV)

Loll, C.M.

1995-09-05

This document presents the 300 Area Treated Effluent Disposal Facility (TEDF) National Pollutant Discharge Elimination System (NPDES) Permit Compliance Monitoring Plan (MP). The MP describes how ongoing monitoring of the TEDF effluent stream for compliance with the NPDES permit will occur. The MP also includes Quality Assurance protocols to be followed.

40 CFR 440.42 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2010 CFR

2010-07-01

... annual precipitation falling on the treatment facility and the drainage area contributing surface runoff... difference between annual precipitation falling on the treatment facility and the drainage area contributing surface runoff to the treatment facility and annual evaporation may be discharged subject to the...

300 Area treated effluent disposal facility sampling schedule. Revision 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Loll, C.M.

1995-03-28

This document is the interface between the 300 Area liquid effluent process engineering (LEPE) group and the waste sampling and characterization facility (WSCF), concerning process control samples. It contains a schedule for process control samples at the 300 Area TEDF which describes the parameters to be measured, the frequency of sampling and analysis, the sampling point, and the purpose for each parameter.

Fate of individual sewage disposal system wastewater within regolith in mountainous terrain

NASA Astrophysics Data System (ADS)

Dano, Kathleen; Poeter, Eileen; Thyne, Geoff

2008-06-01

In order to improve understanding of the fate of septic tank or individual sewage disposal system (ISDS) effluent in regolith overlying fractured-rock aquifers, effluent from an ISDS in such a setting was tracked via geophysical, hydrological, and geochemical methods. Under typical precipitation conditions, the effluent entered the fractured bedrock within 5 m of the boundary of the constructed infiltration area. During a period of unusually high spring recharge, the plume migrated between 50 and 100 m within the regolith before infiltrating the fractured bedrock. The chemical signature of the effluent is similar to that required to account for the decline in water quality, suggesting a causative relationship (as estimated from mass-balance models of the surface-water chemistry near the mouth of the basin). The elevated salt content of the effluent during periods of high natural recharge to the infiltration area correlates with elevated salt concentrations in surface and groundwater at the basin scale, suggesting that some of the effluent salt load may be stored in the unsaturated zone during dry periods and flushed during periods of elevated natural recharge.

Magnetohydrodynamics (MHD) Engineering Test Facility (ETF) 200 MWe power plant Conceptual Design Engineering Report (CDER)

NASA Technical Reports Server (NTRS)

1981-01-01

The reference conceptual design of the magnetohydrodynamic (MHD) Engineering Test Facility (ETF), a prototype 200 MWe coal-fired electric generating plant designed to demonstrate the commercial feasibility of open cycle MHD, is summarized. Main elements of the design, systems, and plant facilities are illustrated. System design descriptions are included for closed cycle cooling water, industrial gas systems, fuel oil, boiler flue gas, coal management, seed management, slag management, plant industrial waste, fire service water, oxidant supply, MHD power ventilating

Magnetohydrodynamics (MHD) Engineering Test Facility (ETF) 200 MWe power plant Conceptual Design Engineering Report (CDER)

NASA Astrophysics Data System (ADS)

1981-09-01

The reference conceptual design of the magnetohydrodynamic (MHD) Engineering Test Facility (ETF), a prototype 200 MWe coal-fired electric generating plant designed to demonstrate the commercial feasibility of open cycle MHD, is summarized. Main elements of the design, systems, and plant facilities are illustrated. System design descriptions are included for closed cycle cooling water, industrial gas systems, fuel oil, boiler flue gas, coal management, seed management, slag management, plant industrial waste, fire service water, oxidant supply, MHD power ventilating

In vivo results of hydrogel composite pericardial substitutes.

PubMed

Blue, M A; Guilbeau, E J; Brandon, T A; Walker, A S; Bjotvedt, G; Fisk, R L

1991-01-01

In this study, two improved pericardial patches were developed and evaluated for their efficacy as pericardial substitutes. The patches are composites consisting of a hydrogel (PHEMA) that coats an underlying mesh (either ETFE or PET). Studies were conducted using subcutaneous implants in rats and pericardial patch implants in greyhound dogs. Adhesions between the substitute and pericardium and the epicardium were minimal. The ETFE composite patch caused an unacceptable epicardial reaction. The PET patch results were encouraging; the epicardium was largely unaffected by the patch.

Rapid Ascent Simulation at NASA-MSFC

NASA Technical Reports Server (NTRS)

Sisco, Jimmy D.

2004-01-01

The Environmental Test Facility (ETF), located at NASA-Marshall Space Flight Center, Huntsville, Alabama, has provided thermal vacuum testing for several major programs since the 1960's. The ETF consists of over 13 thermal vacuum chambers sized and configured to handle the majority of test payloads. The majority of tests require a hard vacuum with heating and cryogenics. NASA's Return-to-Flight program requested testing to simulate a launch from the ground to flight using vacuum, heating and cryogenics. This paper describes an effective method for simulating a launch.

Magnetohydrodynamics (MHD) Engineering Test Facility (ETF) 200 MWe power plant. Design Requirements Document (DRD)

NASA Technical Reports Server (NTRS)

Rigo, H. S.; Bercaw, R. W.; Burkhart, J. A.; Mroz, T. S.; Bents, D. J.; Hatch, A. M.

1981-01-01

A description and the design requirements for the 200 MWe (nominal) net output MHD Engineering Test Facility (ETF) Conceptual Design, are presented. Performance requirements for the plant are identified and process conditions are indicated at interface stations between the major systems comprising the plant. Also included are the description, functions, interfaces and requirements for each of these major systems. The lastest information (1980-1981) from the MHD technology program are integrated with elements of a conventional steam electric power generating plant.

Electrets used to measure exhaust cloud effluents from Solid Rocket Motor (SRM) during demonstration model (DM-2) static test firing

NASA Technical Reports Server (NTRS)

Susko, M.

1978-01-01

Electrets were compared with fixed flow samplers during static test firing. The measurement of the rocket exhaust effluents by samplers and electrets indicated that the Solid Rocket Motor had no significant effect on the air quality in the area sampled. The results show that the electrets (a passive device which needs no power) can be used effectively alongside existing measuring devices (which need power). By placing electrets in areas where no power is available, measurements may be obtained. Consequently, it is a valuable complementary instrument in measuring rocket exhaust effluents in areas where other measuring devices may not be able to assess the contaminants.

78 FR 4170 - License Amendment Request for Analytical Bio-Chemistry Laboratories, Inc., Columbia, MO

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-18

... clay layer. Since the mean concentration of the effluent discharge area of the sanitary lagoon is well... authorize the release of the licensee's sanitary lagoon and the surrounding effluent discharge area for... the west side of the site and comprised approximately 28 acres. The licensee's sanitary lagoon was...

An overview of changes in pressure values of the middle ear using impedance audiometry among diver candidates in a hyperbaric chamber before and after a pressure test

NASA Astrophysics Data System (ADS)

Anoraga, J. S.; Bramantyo, B.; Bardosono, S.; Simanungkalit, S. H.; Basiruddin, J.

2017-08-01

Impedance audiometry is not yet routinely used in pressure tests, especially in Indonesia. Direct exposure to pressure in a hyperbaric chamber is sometimes without any assessment of the middle ear or the Eustachian tube function (ETF) of ventilation. Impedance audiometry examinations are important to assess ETF ventilation. This study determined the middle ear pressure value changes associated with the ETF (ventilation) of prospective divers. This study included 29 prospective divers aged 20-40 years without conductive hearing loss. All subjects underwent a modified diving impedance audiometry examination both before and after the pressure test in a double-lock hyperbaric chamber. Using the Toynbee maneuver, the values obtained for changes of pressure in the middle ear were significant before and after the pressure test in the right and left ears: p < 0.001 and p = 0.018, respectively. The impedance audiometry examination is necessary for the selection of candidate divers undergoing pressure tests within a hyperbaric chamber.

Influence of grafting solvents on the properties of polymer electrolyte membranes prepared by γ-ray preirradiation method

NASA Astrophysics Data System (ADS)

Kimura, Yosuke; Asano, Masaharu; Chen, Jinhua; Maekawa, Yasunari; Katakai, Ryoichi; Yoshida, Masaru

2008-07-01

The effect of grafting solvents, such as isopropanol (iPrOH), tetrachloroethane (TCE), tetrahydrofuran (THF), and toluene, on the preparation of poly(ethylene- co-tetrafluoroethylene)-graft-poly(styrene sulfonic acid) (ETFE-g-PSSA) electrolyte membranes by the γ-ray preirradiation grafting method was investigated. It was found that the iPrOH can drastically accelerate the grafting, resulting in a higher degree of grafting. However, for an appropriate degree of grafting of about 50%, the sulfonic acid groups in the ETFE-g-PSSA membrane prepared with the iPrOH were mainly distributed near the membrane surface, as shown by low proton conductivity in the membrane thickness direction. In contrast to this result, the ETFE-g-PSSA membranes prepared with the THF, toluene and TCE exhibited uniform distribution of the sulfonic acid groups in the membrane. Especially, in the case of the TCE grafting solvent, the chemical stability of the resultant electrolyte membrane was clearly higher than those prepared with the other grafting solvents.

Effect of treated effluent diversion on Yahara River flow, Wisconsin

USGS Publications Warehouse

Young, K.B.

1966-01-01

Before December 1958 the treated sewage effluent from the Madison, Wisconsin, metropolitan area was discharged into the Yahara River at the north end of Lake Waubesa, which is upstream from the USGS gaging station on the Yahara River near McFarland, Wis. Since December 1958 the effluent has been diverted southward from the sewage treatment plant into Badfish Creek and enters the lower reach of Yahara River, thus byrpassing the gaging station. The purpose of this report is to demonstrate the effect that this diversion seems to have on the flow of the Yahara River near McFarland. Indirectly, it also demonstrates the effect on streamflow of withdrawing ground water for use in the Madison metropolitan area since the treated effluent is primarily the major portion of the used ground water.

Imidazolium-based anion exchange membranes for alkaline anion fuel cells: elucidation of the morphology and the interplay between the morphology and properties.

PubMed

Zhao, Yue; Yoshimura, Kimio; Shishitani, Hideyuki; Yamaguchi, Susumu; Tanaka, Hirohisa; Koizumi, Satoshi; Szekely, Noemi; Radulescu, Aurel; Richter, Dieter; Maekawa, Yasunari

2016-02-07

We investigated the morphology and swelling behavior of a new graft-type of anion exchange membrane (AEM) containing 2-methylimidazolium groups by using a contrast variation small angle neutron scattering (SANS) technique. These AEMs were prepared by radiation-induced grafting of 2-methyl-1-vinylimidazole and styrene into poly(ethylene-co-tetrafluoroethylene) (ETFE) films and subsequent N-alkylation with methyliodide, and possessed both high alkaline durability and high conductivity. Our results showed that the crystalline lamellar and crystallite structures originating from the pristine ETFE films were more or less conserved in these AEMs, but the lamellar d-spacing in both dry and wet membranes was enlarged, indicating an expansion of the amorphous lamellae due to the graft chains introduced in the grafting process and the water incorporated in the swelling process. For the first time, the swelling behavior of the AEMs was studied quantitatively in various water mixtures of water and deuterated water with different volume ratios (contrast variation method), and the morphology of these membranes was elucidated by three phases: phase (1) crystalline ETFE domains, which offer good mechanical properties; phase (2) hydrophobic amorphous domains, which are made up of amorphous ETFE chains and offer a matrix to create conducting regions; phase (3) interconnected hydrated domains, which are composed of the entire graft chains and water and play a key role in promoting the conductivity.

Identification of the 2-Hydroxyglutarate and Isovaleryl-CoA Dehydrogenases as Alternative Electron Donors Linking Lysine Catabolism to the Electron Transport Chain of Arabidopsis Mitochondria[W][OA

PubMed Central

Araújo, Wagner L.; Ishizaki, Kimitsune; Nunes-Nesi, Adriano; Larson, Tony R.; Tohge, Takayuki; Krahnert, Ina; Witt, Sandra; Obata, Toshihiro; Schauer, Nicolas; Graham, Ian A.; Leaver, Christopher J.; Fernie, Alisdair R.

2010-01-01

The process of dark-induced senescence in plants is relatively poorly understood, but a functional electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) complex, which supports respiration during carbon starvation, has recently been identified. Here, we studied the responses of Arabidopsis thaliana mutants deficient in the expression of isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase to extended darkness and other environmental stresses. Evaluations of the mutant phenotypes following carbon starvation induced by extended darkness identify similarities to those exhibited by mutants of the ETF/ETFQO complex. Metabolic profiling and isotope tracer experimentation revealed that isovaleryl-CoA dehydrogenase is involved in degradation of the branched-chain amino acids, phytol, and Lys, while 2-hydroxyglutarate dehydrogenase is involved exclusively in Lys degradation. These results suggest that isovaleryl-CoA dehydrogenase is the more critical for alternative respiration and that a series of enzymes, including 2-hydroxyglutarate dehydrogenase, plays a role in Lys degradation. Both physiological and metabolic phenotypes of the isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase mutants were not as severe as those observed for mutants of the ETF/ETFQO complex, indicating some functional redundancy of the enzymes within the process. Our results aid in the elucidation of the pathway of plant Lys catabolism and demonstrate that both isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase act as electron donors to the ubiquinol pool via an ETF/ETFQO-mediated route. PMID:20501910

Identification of the 2-hydroxyglutarate and isovaleryl-CoA dehydrogenases as alternative electron donors linking lysine catabolism to the electron transport chain of Arabidopsis mitochondria.

PubMed

Araújo, Wagner L; Ishizaki, Kimitsune; Nunes-Nesi, Adriano; Larson, Tony R; Tohge, Takayuki; Krahnert, Ina; Witt, Sandra; Obata, Toshihiro; Schauer, Nicolas; Graham, Ian A; Leaver, Christopher J; Fernie, Alisdair R

2010-05-01

The process of dark-induced senescence in plants is relatively poorly understood, but a functional electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) complex, which supports respiration during carbon starvation, has recently been identified. Here, we studied the responses of Arabidopsis thaliana mutants deficient in the expression of isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase to extended darkness and other environmental stresses. Evaluations of the mutant phenotypes following carbon starvation induced by extended darkness identify similarities to those exhibited by mutants of the ETF/ETFQO complex. Metabolic profiling and isotope tracer experimentation revealed that isovaleryl-CoA dehydrogenase is involved in degradation of the branched-chain amino acids, phytol, and Lys, while 2-hydroxyglutarate dehydrogenase is involved exclusively in Lys degradation. These results suggest that isovaleryl-CoA dehydrogenase is the more critical for alternative respiration and that a series of enzymes, including 2-hydroxyglutarate dehydrogenase, plays a role in Lys degradation. Both physiological and metabolic phenotypes of the isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase mutants were not as severe as those observed for mutants of the ETF/ETFQO complex, indicating some functional redundancy of the enzymes within the process. Our results aid in the elucidation of the pathway of plant Lys catabolism and demonstrate that both isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase act as electron donors to the ubiquinol pool via an ETF/ETFQO-mediated route.

Low-autofluorescence fluoropolymer membrane filters for cell filtration

NASA Astrophysics Data System (ADS)

Kihara, Naoto; Kuboyama, Daiki; Onoshima, Daisuke; Ishikawa, Kenji; Tanaka, Hiromasa; Ozawa, Naoya; Hase, Tetsunari; Koguchi, Ryohei; Yukawa, Hiroshi; Odaka, Hidefumi; Hasegawa, Yoshinori; Baba, Yoshinobu; Hori, Masaru

2018-06-01

A fluoropolymer membrane filter with through-holes was fabricated by photolithographic patterning and the dry etching method. 380,000 highly packed through-holes, each with a diameter of 7 µm were able to cover a whole area with a diameter of 13 mm. Ethylene tetrafluoroethylene (ETFE) was used as the membrane, which was suitable for the fluorescence detection of rare cells such as circulating tumor cells (CTCs) in human blood. The device fabrication for the size based capture of rare cells in blood such as CTCs is realized in this study.

Coupling in vitro and in vivo neurochemical-based assessments of wastewater effluents from the Maumee River Area of Concern (AOC)

EPA Science Inventory

Here we utilize in vivo and in vitro approaches to study whether real world effluents released in the Maumee River (Toledo, OH) Area of Concern (AOC) contain neuroactive substances that may impair fish reproduction and behavior. Our approaches help extend the concept of endocrine...

History of Allied Force Headquarters - Part Two. December 1942 - December 1943, Section 4

DTIC Science & Technology

1943-01-01

NATOUSA-signal organizations, endlto maintain adequate sta tistical date reflecting the status of Theater signal stocks . (3) To supervise the...Chaplain ISection NATOUSA from 4 February to 8 24 March 1943. He was promoted to the rank of lieuten- 1. AFHQ, memo to CG’s EAF, ETF , CTF, WTF, 12th AF...may be authorized for sale by 62. AFEQ, memo! to CG’s EAF, ETF , CTF, VVTF, 12th AF, and Hq Comdt, 3 Oct󈧮 (AG AFEQ file: 321-10). 63. NATOUSA GO No

Design studies on the 4π γ-ray calorimeter for the ETF experiment at HIRFL-CSR

NASA Astrophysics Data System (ADS)

Yue, Ke; Xu, Hu-Shan; Sun, Zhi-Yu; Su, Guang-Hui; Wang, Jian-Song; Zheng, Chuan; Li, Song-Lin; Hu, Zheng-Guo; Chen, Rou-Fu; Xiao, Zhi-Gang; Hu, Qiang; Zhang, Xue-Ying; Yu, Yu-Hong; Chen, Jun-Ling

2011-01-01

A high detection efficiency calorimeter which is used to detect γ-rays with energies from 1 MeV up to 10 MeV as well as light charged particles has been proposed. Design of the geometry, results of the crystal tests and Monte Carlo simulations are presented in this paper. The simulation results confirm that the calorimeter can obtain high detection efficiency and good energy resolution with the current designed geometry. And the calorimeter is competent for the future External Target Facility (ETF) experiments.

Approach to ignition of tokamak reactors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sigmar, D.J.

1981-02-01

Recent transport modeling results for JET, INTOR, and ETF are reviewed and analyzed with respect to existing uncertainties in the underlying physics, the self-consistency of the very large numerical codes, and the margin for ignition. The codes show ignition to occur in ETF/INTOR-sized machines if empirical scaling can be extrapolated to ion temperatures (and beta values) much higher than those presently achieved, if there is no significant impurity accumulation over the first 7 s, and if the known ideal and resistive MHD instabilities remain controllable for the evolving plasma profiles during ignition startup.

Application of the finite element groundwater model FEWA to the engineered test facility

DOE Office of Scientific and Technical Information (OSTI.GOV)

Craig, P.M.; Davis, E.C.

1985-09-01

A finite element model for water transport through porous media (FEWA) has been applied to the unconfined aquifer at the Oak Ridge National Laboratory Solid Waste Storage Area 6 Engineered Test Facility (ETF). The model was developed in 1983 as part of the Shallow Land Burial Technology - Humid Task (ONL-WL14) and was previously verified using several general hydrologic problems for which an analytic solution exists. Model application and calibration, as described in this report, consisted of modeling the ETF water table for three specialized cases: a one-dimensional steady-state simulation, a one-dimensional transient simulation, and a two-dimensional transient simulation. Inmore » the one-dimensional steady-state simulation, the FEWA output accurately predicted the water table during a long period in which there were no man-induced or natural perturbations to the system. The input parameters of most importance for this case were hydraulic conductivity and aquifer bottom elevation. In the two transient cases, the FEWA output has matched observed water table responses to a single rainfall event occurring in February 1983, yielding a calibrated finite element model that is useful for further study of additional precipitation events as well as contaminant transport at the experimental site.« less

River capture and sediment redistribution in northern Tunisia: The doom of Utica

NASA Astrophysics Data System (ADS)

Booth-Rea, Guillermo; Camafort, Miquel; Pérez-Peña, J. Vicente; Melki, Fetheddine; Ranero, César; Azañón, José Miguel; Gracia, Eulalia; Ouadday, Mohamed

2016-04-01

Utica was a flourishing port city in northern Tunisia since the Phoenician times, 12-9th century B.C., until the 4th century A.D.. However, at present it is located 10 km from the coastline after very fast late Holocene progradation of the Mejerda River delta into the bay of Utica. This fast delta progradation occurred after Mejerda River captured Tine River increasing 140 % the river catchment area. Charcoal fragments present in the youngest Tine river terrace at the wind gap give a conventional radiocarbon age of 3240 +/- 30yr BP, indicating that the capture occurred after this date. Quaternary fluvial terraces located in the Tine River paleovalley have been folded and uplifted above a fold related to the active El Alia Tebousouk reverse fault (ETF). Continued uplift of the Tine River valley above the ETF favoured headward erosion of the Medjerda river tributaries creating a transverse drainage that captured Tine River. This capture produced an important change in sediment discharge along the northern Tunisia coast driving sediments to the Gulf of Tunis instead of feeding the Tyrrhenian Sea through the Ichkeul and Bizerte lakes. Although anthropogenic derived degradation of northern Tunisia land for agricultural purposes probably influenced the increase in sediment into the Utica bay, the main cause of rapid progradation of the Medjerda River delta during the late Holocene is related to its increase in drainage area after capturing the Tine River. This process was mostly driven by local contractive tectonics linked to the seismogenic Alia Tebousouk reverse fault.

Characterization of fish hold effluent discharged from commercial fishing vessels into harbor waters.

PubMed

Albert, Ryan J; McLaughlin, Christine; Falatko, Debra

2014-10-15

Fish hold effluent and the effluent produced from the cleaning of fish holds may contain organic material resulting from the degradation of seafood and cleaning products (e.g., soaps and detergents). This effluent is often discharged by vessels into near shore waters and, therefore, could have the potential to contribute to water pollution in bays and estuaries. We characterized effluent from commercial fishing vessels with holds containing refrigerated seawater, ice slurry, or chipped ice. Concentrations of trace heavy metals, wet chemistry parameters, and nutrients in effluent were compared to screening benchmarks to determine if there is a reasonable potential for effluent discharge to contribute to nonattainment of water quality standards. Most analytes (67%) exceeded their benchmark concentration and, therefore, may have the potential to pose risk to human health or the environment if discharges are in significant quantities or there are many vessels discharging in the same areas. Published by Elsevier Ltd.

Ground-Water Flow, 2004-07, and Water Quality, 1992-2007, in McBaine Bottoms, Columbia, Missouri

USGS Publications Warehouse

Smith, Brenda Joyce; Richards, Joseph M.

2008-01-01

The U.S. Geological Survey, in cooperation with the city of Columbia, Missouri, and the Missouri Department of Conservation, collected ground-water quality data, surface-water quality data, and water-level data in McBaine Bottoms, southwest of Columbia. McBaine Bottoms, adjacent to the Missouri River, is the location of the municipal-supply well field for the city of Columbia, the city of Columbia wastewater-treatment wetlands, and the Missouri Department of Conservation Eagle Bluffs Conservation Area. This report describes the ground-water flow and water quality of McBaine Bottoms and provides information to better understand the interaction between treated effluent from the wetlands used on the Eagle Bluffs Conservation Area and the water in the alluvial aquifer that is pumped from the city of Columbia municipal-supply well field. Changes in major chemical constituent concentrations have been detected at several sampling sites between pre- and post-effluent application data. Analysis of post-effluent data indicates substantial changes in calcium, potassium, sodium, chloride, and sulfate concentrations in ground water. These changes became apparent shortly after the beginning of the operation of the wastewater-treatment wetland in 1994 and the formation of the Eagle Bluffs Conservation Area, which uses the treated effluent as a water source for the management of migratory water fowl. The changes have continued throughout the 15 years of sample collection. The concentrations of these major chemical constituents are on the mixing continuum between pre-effluent ground water as one end member and the treated wastewater effluent as the other end member. For monitoring wells that had changes in major chemical constituent concentrations, the relative percentage of treated effluent in the ground water, assuming chloride is conservative, ranged from 6 to 88 percent. Twenty-two monitoring wells throughout McBaine Bottoms have been affected by effluent based on chloride concentrations larger than 40 milligrams per liter. The chloride concentration of ground water in the alluvial aquifer reflects several sources, including precipitation, water from the Missouri River, water in the aquifer, and the treated effluent. Chloride concentrations from precipitation, the Missouri River, and water in the alluvial aquifer were less than 40 milligrams per liter. These monitoring wells affected by effluent are located in two general areas - adjacent to treatment wetland unit 1 and near the ground-water high on and north of the Eagle Bluffs Conservation Area. The probable source of the large chloride concentrations in well samples adjacent to treatment wetland unit 1 is leakage from the unit. The source for the large chloride concentrations in the other monitoring well samples is the effluent mixed with ground water and Missouri River water that is used to fill pools on the Eagle Bluffs Conservation Area. One monitoring well had a single sample with a chloride concentration larger than 40 milligrams per liter. That sample may have been affected by the use of road salt because of the presence of ice and snow immediately before the sample was collected. Lateral ground-water flow was dominated by the presence of a persistent ground-water high beneath the Eagle Bluffs Conservation Area and the presence of a cone of depression centered around the city of Columbia well field in the northern part of the study area. Ground-water flow was radially away from the apex of the ground-water high; west and south of the high, flow was toward the Missouri River, east of the high, flow was toward Perche Creek, and north of the high, flow was to the north toward the cone of depression around the city of Columbia well field. Another permanent feature on the water-level maps was a ground-water high beneath treatment wetland unit 1. Although the ground-water high was present throughout the study period, the subsurface expression of the high changed depending on hydrolo

Atmospheric Effects and Potential Climatic Impact of the 1980 Eruptions of Mount St. Helens

NASA Technical Reports Server (NTRS)

Deepak, A. (Editor)

1982-01-01

Measurements and studies of the 1980 Mount St. Helens volcanic eruptions and their atmospheric effects and climatic impact are addressed. Specific areas discussed include: (1) nature and impact of volcanic eruptions; (2) in situ measurements of effluents; (3) remote sensing measurements; (4) transport and dispersion of volcanic effluents; (5) chemistry of volcanic effluents; and (6) weather and potential climate impact.

The Critical Role of Arabidopsis Electron-Transfer Flavoprotein:Ubiquinone Oxidoreductase during Dark-Induced StarvationW⃞

PubMed Central

Ishizaki, Kimitsune; Larson, Tony R.; Schauer, Nicolas; Fernie, Alisdair R.; Graham, Ian A.; Leaver, Christopher J.

2005-01-01

In mammals, electron-transfer flavoprotein:ubiquinone oxidoreductase (ETFQO) and electron-transfer flavoprotein (ETF) are functionally associated, and ETF accepts electrons from at least nine mitochondrial matrix flavoprotein dehydrogenases and transfers them to ubiquinone in the inner mitochondrial membrane. In addition, the mammalian ETF/ETFQO system plays a key role in β-oxidation of fatty acids and catabolism of amino acids and choline. By contrast, nothing is known of the function of ETF and ETFQO in plants. Sequence analysis of the unique Arabidopsis thaliana homologue of ETFQO revealed high similarity to the mammalian ETFQO protein. Moreover, green fluorescent protein cellular localization experiments suggested a mitochondrial location for this protein. RNA gel blot analysis revealed that Arabidopsis ETFQO transcripts accumulated in long-term dark-treated leaves. Analysis of three independent insertional mutants of Arabidopsis ETFQO revealed a dramatic reduction in their ability to withstand extended darkness, resulting in senescence and death within 10 d after transfer, whereas wild-type plants remained viable for at least 15 d. Metabolite profiling of dark-treated leaves of the wild type and mutants revealed a dramatic decline in sugar levels. In contrast with the wild type, the mutants demonstrated a significant accumulation of several amino acids, an intermediate of Leu catabolism, and, strikingly, high-level accumulation of phytanoyl-CoA. These data demonstrate the involvement of a mitochondrial protein, ETFQO, in the catabolism of Leu and potentially of other amino acids in higher plants and also imply a novel role for this protein in the chlorophyll degradation pathway activated during dark-induced senescence and sugar starvation. PMID:16055629

Studies on the mechanism of electron bifurcation catalyzed by electron transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) of Acidaminococcus fermentans.

PubMed

Chowdhury, Nilanjan Pal; Mowafy, Amr M; Demmer, Julius K; Upadhyay, Vikrant; Koelzer, Sebastian; Jayamani, Elamparithi; Kahnt, Joerg; Hornung, Marco; Demmer, Ulrike; Ermler, Ulrich; Buckel, Wolfgang

2014-02-21

Electron bifurcation is a fundamental strategy of energy coupling originally discovered in the Q-cycle of many organisms. Recently a flavin-based electron bifurcation has been detected in anaerobes, first in clostridia and later in acetogens and methanogens. It enables anaerobic bacteria and archaea to reduce the low-potential [4Fe-4S] clusters of ferredoxin, which increases the efficiency of the substrate level and electron transport phosphorylations. Here we characterize the bifurcating electron transferring flavoprotein (EtfAf) and butyryl-CoA dehydrogenase (BcdAf) of Acidaminococcus fermentans, which couple the exergonic reduction of crotonyl-CoA to butyryl-CoA to the endergonic reduction of ferredoxin both with NADH. EtfAf contains one FAD (α-FAD) in subunit α and a second FAD (β-FAD) in subunit β. The distance between the two isoalloxazine rings is 18 Å. The EtfAf-NAD(+) complex structure revealed β-FAD as acceptor of the hydride of NADH. The formed β-FADH(-) is considered as the bifurcating electron donor. As a result of a domain movement, α-FAD is able to approach β-FADH(-) by about 4 Å and to take up one electron yielding a stable anionic semiquinone, α-FAD, which donates this electron further to Dh-FAD of BcdAf after a second domain movement. The remaining non-stabilized neutral semiquinone, β-FADH(•), immediately reduces ferredoxin. Repetition of this process affords a second reduced ferredoxin and Dh-FADH(-) that converts crotonyl-CoA to butyryl-CoA.

An Eulerian time filtering technique to study large-scale transient flow phenomena

NASA Astrophysics Data System (ADS)

Vanierschot, Maarten; Persoons, Tim; van den Bulck, Eric

2009-10-01

Unsteady fluctuating velocity fields can contain large-scale periodic motions with frequencies well separated from those of turbulence. Examples are the wake behind a cylinder or the processing vortex core in a swirling jet. These turbulent flow fields contain large-scale, low-frequency oscillations, which are obscured by turbulence, making it impossible to identify them. In this paper, we present an Eulerian time filtering (ETF) technique to extract the large-scale motions from unsteady statistical non-stationary velocity fields or flow fields with multiple phenomena that have sufficiently separated spectral content. The ETF method is based on non-causal time filtering of the velocity records in each point of the flow field. It is shown that the ETF technique gives good results, similar to the ones obtained by the phase-averaging method. In this paper, not only the influence of the temporal filter is checked, but also parameters such as the cut-off frequency and sampling frequency of the data are investigated. The technique is validated on a selected set of time-resolved stereoscopic particle image velocimetry measurements such as the initial region of an annular jet and the transition between flow patterns in an annular jet. The major advantage of the ETF method in the extraction of large scales is that it is computationally less expensive and it requires less measurement time compared to other extraction methods. Therefore, the technique is suitable in the startup phase of an experiment or in a measurement campaign where several experiments are needed such as parametric studies.

Studies on the Mechanism of Electron Bifurcation Catalyzed by Electron Transferring Flavoprotein (Etf) and Butyryl-CoA Dehydrogenase (Bcd) of Acidaminococcus fermentans*

PubMed Central

Chowdhury, Nilanjan Pal; Mowafy, Amr M.; Demmer, Julius K.; Upadhyay, Vikrant; Koelzer, Sebastian; Jayamani, Elamparithi; Kahnt, Joerg; Hornung, Marco; Demmer, Ulrike; Ermler, Ulrich; Buckel, Wolfgang

2014-01-01

Electron bifurcation is a fundamental strategy of energy coupling originally discovered in the Q-cycle of many organisms. Recently a flavin-based electron bifurcation has been detected in anaerobes, first in clostridia and later in acetogens and methanogens. It enables anaerobic bacteria and archaea to reduce the low-potential [4Fe-4S] clusters of ferredoxin, which increases the efficiency of the substrate level and electron transport phosphorylations. Here we characterize the bifurcating electron transferring flavoprotein (EtfAf) and butyryl-CoA dehydrogenase (BcdAf) of Acidaminococcus fermentans, which couple the exergonic reduction of crotonyl-CoA to butyryl-CoA to the endergonic reduction of ferredoxin both with NADH. EtfAf contains one FAD (α-FAD) in subunit α and a second FAD (β-FAD) in subunit β. The distance between the two isoalloxazine rings is 18 Å. The EtfAf-NAD+ complex structure revealed β-FAD as acceptor of the hydride of NADH. The formed β-FADH− is considered as the bifurcating electron donor. As a result of a domain movement, α-FAD is able to approach β-FADH− by about 4 Å and to take up one electron yielding a stable anionic semiquinone, α-FAD⨪, which donates this electron further to Dh-FAD of BcdAf after a second domain movement. The remaining non-stabilized neutral semiquinone, β-FADH•, immediately reduces ferredoxin. Repetition of this process affords a second reduced ferredoxin and Dh-FADH− that converts crotonyl-CoA to butyryl-CoA. PMID:24379410

Identification and characterization of cell-specific enhancer elements for the mouse ETF/Tead2 gene.

PubMed

Tanoue, Y; Yasunami, M; Suzuki, K; Ohkubo, H

2001-12-21

We have identified and characterized by transient transfection assays the cell-specific 117-bp enhancer sequence in the first intron of the mouse ETF (Embryonic TEA domain-containing factor)/Tead2 gene required for transcriptional activation in ETF/Tead2 gene-expressing cells, such as P19 cells. The 117-bp enhancer contains one GC-rich sequence (5'-GGGGCGGGG-3'), termed the GC box, and two tandemly repeated GA-rich sequences (5'-GGGGGAGGGG-3'), termed the proximal and distal GA elements. Further analyses, including transfection studies and electrophoretic mobility shift assays using a series of deletion and mutation constructs, indicated that Sp1, a putative activator, may be required to predominate over its competition with another unknown putative repressor, termed the GA element-binding factor, for binding to both the GC box, which overlapped with the proximal GA element, and the distal GA element in the 117-bp sequence in order to achieve a full enhancer activity. We also discuss a possible mechanism underlying the cell-specific enhancer activity of the 117-bp sequence.

Singapore National Medical Image Resource Centre (SN.MIRC): a world wide web resource for radiology education.

PubMed

Yang, Guo-Liang; Lim, C C Tchoyoson

2006-08-01

Radiology education is heavily dependent on visual images, and case-based teaching files comprising medical images can be an important tool for teaching diagnostic radiology. Currently, hardcopy film is being rapidly replaced by digital radiological images in teaching hospitals, and an electronic teaching file (ETF) library would be desirable. Furthermore, a repository of ETFs deployed on the World Wide Web has the potential for e-learning applications to benefit a larger community of learners. In this paper, we describe a Singapore National Medical Image Resource Centre (SN.MIRC) that can serve as a World Wide Web resource for teaching diagnostic radiology. On SN.MIRC, ETFs can be created using a variety of mechanisms including file upload and online form-filling, and users can search for cases using the Medical Image Resource Center (MIRC) query schema developed by the Radiological Society of North America (RSNA). The system can be improved with future enhancements, including multimedia interactive teaching files and distance learning for continuing professional development. However, significant challenges exist when exploring the potential of using the World Wide Web for radiology education.

Water reuse at highway rest stations.

DOT National Transportation Integrated Search

1974-01-01

A laboratory biological wastewater treatment system was operated to investigate the effects of wastewater effluent recycle on the treatment system and the effluent water quality. This concept is being investigated for use at highway rest areas in the...

Constructed wetlands and waste stabilization ponds for small rural communities in the United Kingdom: a comparison of land area requirements, performance and costs.

PubMed

Mara, D D

2006-07-01

Land area requirements for secondary subsurface horizontal-flow constructed wetlands (CW) and primary and secondary facultative ponds with either unaerated or aerated rock filters were determined for three levels of effluent quality: that specified in the Urban Waste Water Treatment Directive (UWWTD) (< or = 25 mg filtered BOD l(-1) and < or = 150 mg SS l(-1) for waste stabilization ponds (WSP) effluents, and < or = 25 mg unfiltered BOD l(-1) for CW effluents (mean values); and two common requirements of the Environment Agency: < or = 40 mg BOD l(-1) and < or = 60 mg SS l(-1), and < or = 10 mg BOD l(-1), < or = 15 mg SS l(-1) and < or = 5 mg ammonia-N l(-1) (95-percentile values). A secondary CW requires 60 percent more land than a secondary facultative pond to produce an UWWTD-quality effluent, 38 percent more land than a secondary facultative pond and an unaerated rock filter to produce a 40/60 effluent and, were it to be used to produce a 10/15/5 effluent, it would require approximately 480 percent more land than a secondary facultative pond and an aerated rock filter. Its estimated 2005 cost is pound 1100-2600 p.e.(-1), whereas that of a primary facultative pond and rock filter is approximately pound 400 p.e.(-1). On the basis of land area requirements, performance and cost, facultative ponds and unaerated or aerated rock filters are to be preferred to secondary subsurface horizontal-flow constructed wetlands.

A new technique of extracapsular restoration with a tie made of ethylene tetrafluoroethylene (ETFE) for rupture of the cranial cruciate ligament in dogs.

PubMed

Yamada, E; Imayama, Y; Katano, S; Nagashima, F; Shibata, T

1996-06-01

A new extracapsular technique for repair of canine cranial cruciate ligament rupture using an ethylene tetrafluoroethylene (ETFE) tie was presented. Eighteen dogs (body weight: 6.0-46 kg) with this problem were used for this study. The advantages of this method were 1) the operation was easily performed. 2) Joint could be stabilized by proper fixation with microadjustment during operation. 3) This method presented less surgical invasion than the intracapsular one, since wide incision was not conducted over peripheral tissue of the stifle joint.

Electrocoagulation for the treatment of textile industry effluent--a review.

PubMed

Khandegar, V; Saroha, Anil K

2013-10-15

Various techniques such as physical, chemical, biological, advanced oxidation and electrochemical are used for the treatment of industrial effluent. The commonly used conventional biological treatment processes are time consuming, need large operational area and are not effective for effluent containing toxic elements. Advanced oxidation techniques result in high treatment cost and are generally used to obtain high purity grade water. The chemical coagulation technique is slow and generates large amount of sludge. Electrocoagulation has recently attracted attention as a potential technique for treating industrial effluent due to its versatility and environmental compatibility. This technique uses direct current source between metal electrodes immersed in the effluent, which causes the dissolution of electrode plates into the effluent. The metal ions, at an appropriate pH, can form wide range of coagulated species and metal hydroxides that destabilize and aggregate particles or precipitate and adsorb the dissolved contaminants. Therefore, the objective of the present manuscript is to review the potential of electrocoagulation for the treatment of industrial effluents, mainly removal of dyes from textile effluent. © 2013 Elsevier Ltd. All rights reserved.

[Computed tomographic measurement of coronary artery calcification in the assessment of cardiovascular risk: a descriptive study].

PubMed

Morcillo, César; Valderas, José M; Roca, Joan M; Oliveró, Ruperto; Núñez, Cristina; Sánchez, Mónica; Bechich, Siraj

2007-03-01

Measurement of coronary artery calcification (CAC) is used in the evaluation of cardiovascular risk. We investigated its usefulness by comparing CAC assessment with that of various risk charts. We determined cardiovascular risk in patients without known atherosclerosis using the 1998 European Task Force (ETF), REGICOR (Registre Gironí del Corazón) and SCORE (Systematic Coronary Risk Evaluation) charts. CAC was assessed by computerized tomography and measurements were classified as low risk (i.e., score <1), intermediate risk (i.e., score 1-100), or high risk (i.e., score >100). The study included 331 patients (mean age 54 [8.5] years, 89% male). In 44.1%, CAC was detected (mean score 96 [278]). The degree of agreement between the cardiovascular risk derived from the CAC score and that derived from the SCORE and ETF charts was acceptable: kappa=.33 (P<.05) and kappa=.28 (P<.05), respectively, but agreement was poor with the REGICOR chart: kappa=.02 (P=.32). The SCORE and ETF charts, respectively, classified 45.0% and 38.3% of patients with a CAC score >100 as high risk, whereas the REGICOR chart did not classify any of these patients as high risk. Male sex, older age, smoking history, and a family history of coronary heart disease were all associated with the detection of CAC. Measurement of CAC demonstrated calcification in 44.1% of patients without known atherosclerosis. By regarding those with a CAC score > 100 as high-risk, 10.4% of patients evaluated using the SCORE chart would be reclassified as high risk, as would 11.6% of those evaluated using the ETF chart, and 18.9% of those evaluated using the REGICOR chart. Consequently, more patients would be eligible for preventative treatment.

Analysis of a Range of Catabolic Mutants Provides Evidence That Phytanoyl-Coenzyme A Does Not Act as a Substrate of the Electron-Transfer Flavoprotein/Electron-Transfer Flavoprotein:Ubiquinone Oxidoreductase Complex in Arabidopsis during Dark-Induced Senescence1[W][OA

PubMed Central

Araújo, Wagner L.; Ishizaki, Kimitsune; Nunes-Nesi, Adriano; Tohge, Takayuki; Larson, Tony R.; Krahnert, Ina; Balbo, Ilse; Witt, Sandra; Dörmann, Peter; Graham, Ian A.; Leaver, Christopher J.; Fernie, Alisdair R.

2011-01-01

The process of dark-induced senescence in plants is not fully understood, however, the functional involvement of an electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO), has been demonstrated. Recent studies have revealed that the enzymes isovaleryl-coenzyme A (CoA) dehydrogenase and 2-hydroxyglutarate dehydrogenase act as important electron donors to this complex. In addition both enzymes play a role in the breakdown of cellular carbon storage reserves with isovaleryl-CoA dehydrogenase being involved in degradation of the branched-chain amino acids, phytol, and lysine while 2-hydroxyglutarate dehydrogenase is exclusively involved in lysine degradation. Given that the chlorophyll breakdown intermediate phytanoyl-CoA accumulates dramatically both in knockout mutants of the ETF/ETFQO complex and of isovaleryl-CoA dehydrogenase following growth in extended dark periods we have investigated the direct importance of chlorophyll breakdown for the supply of carbon and electrons during this process. For this purpose we isolated three independent Arabidopsis (Arabidopsis thaliana) knockout mutants of phytanoyl-CoA 2-hydroxylase and grew them under the same extended darkness regime as previously used. Despite the fact that these mutants accumulated phytanoyl-CoA and also 2-hydroxyglutarate they exhibited no morphological changes in comparison to the other mutants previously characterized. These results are consistent with a single entry point of phytol breakdown into the ETF/ETFQO system and furthermore suggest that phytol is not primarily metabolized by this pathway. Furthermore analysis of isovaleryl-CoA dehydrogenase/2-hydroxyglutarate dehydrogenase double mutants generated here suggest that these two enzymes essentially account for the entire electron input via the ETF complex. PMID:21788362

Electron spin relaxation enhancement measurements of interspin distances in human, porcine, and Rhodobacter electron transfer flavoprotein ubiquinone oxidoreductase (ETF QO)

NASA Astrophysics Data System (ADS)

Fielding, Alistair J.; Usselman, Robert J.; Watmough, Nicholas; Simkovic, Martin; Frerman, Frank E.; Eaton, Gareth R.; Eaton, Sandra S.

2008-02-01

Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) is a membrane-bound electron transfer protein that links primary flavoprotein dehydrogenases with the main respiratory chain. Human, porcine, and Rhodobacter sphaeroides ETF-QO each contain a single [4Fe-4S] 2+,1+ cluster and one equivalent of FAD, which are diamagnetic in the isolated enzyme and become paramagnetic on reduction with the enzymatic electron donor or with dithionite. The anionic flavin semiquinone can be reduced further to diamagnetic hydroquinone. The redox potentials for the three redox couples are so similar that it is not possible to poise the proteins in a state where both the [4Fe-4S] + cluster and the flavoquinone are fully in the paramagnetic form. Inversion recovery was used to measure the electron spin-lattice relaxation rates for the [4Fe-4S] + between 8 and 18 K and for semiquinone between 25 and 65 K. At higher temperatures the spin-lattice relaxation rates for the [4Fe-4S] + were calculated from the temperature-dependent contributions to the continuous wave linewidths. Although mixtures of the redox states are present, it was possible to analyze the enhancement of the electron spin relaxation of the FAD semiquinone signal due to dipolar interaction with the more rapidly relaxing [4Fe-4S] + and obtain point-dipole interspin distances of 18.6 ± 1 Å for the three proteins. The point-dipole distances are within experimental uncertainty of the value calculated based on the crystal structure of porcine ETF-QO when spin delocalization is taken into account. The results demonstrate that electron spin relaxation enhancement can be used to measure distances in redox poised proteins even when several redox states are present.

Electron Spin Relaxation Enhancement Measurements of Interspin Distances in Human, Porcine, and Rhodobacter Electron Transfer Flavoprotein-ubiquinone Oxidoreductase (ETF-QO)

PubMed Central

Fielding, Alistair J.; Usselman, Robert J.; Watmough, Nicholas; Simkovic, Martin; Frerman, Frank E.; Eaton, Gareth R.; Eaton, Sandra S.

2008-01-01

Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) is a membrane-bound electron transfer protein that links primary flavoprotein dehydrogenases with the main respiratory chain. Human, porcine, and Rhodobacter sphaeroides ETF-QO each contain a single [4Fe-4S]2+,1+ cluster and one equivalent of FAD, which are diamagnetic in the isolated enzyme and become paramagnetic on reduction with the enzymatic electron donor or with dithionite. The anionic flavin semiquinone can be reduced further to diamagnetic hydroquinone. The redox potentials for the three redox couples are so similar that it is not possible to poise the proteins in a state where both the [4Fe-4S]+ cluster and the flavoquinone are fully in the paramagnetic form. Inversion recovery was used to measure the electron spin-lattice relaxation rates for the [4Fe-4S]+ between 8 and 18 K and for semiquinone between 25 and 65 K. At higher temperatures the spin-lattice relaxation rates for the [4Fe-4S]+ were calculated from the temperature-dependent contributions to the continuous wave linewidths. Although mixtures of the redox states are present, it was possible to analyze the enhancement of the electron spin relaxation of the FAD semiquinone signal due to dipolar interaction with the more rapidly relaxing [4Fe-4S]+ and obtain point dipole interspin distances of 18.6 ± 1 Å for the three proteins. The point-dipole distances are within experimental uncertainty of the value calculated based on the crystal structure of porcine ETF-QO when spin delocalization is taken into account. The results demonstrate that electron spin relaxation enhancement can be used to measure distances in redox poised proteins even when several redox states are present. PMID:18037314

Electron spin relaxation enhancement measurements of interspin distances in human, porcine, and Rhodobacter electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO).

PubMed

Fielding, Alistair J; Usselman, Robert J; Watmough, Nicholas; Simkovic, Martin; Frerman, Frank E; Eaton, Gareth R; Eaton, Sandra S

2008-02-01

Electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) is a membrane-bound electron transfer protein that links primary flavoprotein dehydrogenases with the main respiratory chain. Human, porcine, and Rhodobacter sphaeroides ETF-QO each contain a single [4Fe-4S](2+,1+) cluster and one equivalent of FAD, which are diamagnetic in the isolated enzyme and become paramagnetic on reduction with the enzymatic electron donor or with dithionite. The anionic flavin semiquinone can be reduced further to diamagnetic hydroquinone. The redox potentials for the three redox couples are so similar that it is not possible to poise the proteins in a state where both the [4Fe-4S](+) cluster and the flavoquinone are fully in the paramagnetic form. Inversion recovery was used to measure the electron spin-lattice relaxation rates for the [4Fe-4S](+) between 8 and 18K and for semiquinone between 25 and 65K. At higher temperatures the spin-lattice relaxation rates for the [4Fe-4S](+) were calculated from the temperature-dependent contributions to the continuous wave linewidths. Although mixtures of the redox states are present, it was possible to analyze the enhancement of the electron spin relaxation of the FAD semiquinone signal due to dipolar interaction with the more rapidly relaxing [4Fe-4S](+) and obtain point-dipole interspin distances of 18.6+/-1A for the three proteins. The point-dipole distances are within experimental uncertainty of the value calculated based on the crystal structure of porcine ETF-QO when spin delocalization is taken into account. The results demonstrate that electron spin relaxation enhancement can be used to measure distances in redox poised proteins even when several redox states are present.

Sites of superoxide and hydrogen peroxide production during fatty acid oxidation in rat skeletal muscle mitochondria

PubMed Central

Perevoshchikova, Irina V.; Quinlan, Casey L.; Orr, Adam L.; Gerencser, Akos A.; Brand, Martin D.

2013-01-01

H2O2 production by skeletal muscle mitochondria oxidizing palmitoylcarnitine was examined under two conditions: the absence of respiratory chain inhibitors and the presence of myxothiazol to inhibit complex III. Without inhibitors, respiration and H2O2 production were low unless carnitine or malate was added to limit acetyl-CoA accumulation. With palmitoylcarnitine alone, H2O2 production was dominated by complex II (44% from site IIF in the forward reaction); the remainder was mostly from complex I (34%, superoxide from site IF). With added carnitine, H2O2 production was about equally shared between complexes I, II, and III. With added malate, it was 75% from complex III (superoxide from site IIIQo) and 25% from site IF. Thus complex II (site IIF in the forward reaction) is a major source of H2O2 production during oxidation of palmitoylcarnitine ± carnitine. Under the second condition (myxothiazol present to keep ubiquinone reduced), the rates of H2O2 production were highest in the presence of palmitoylcarnitine ± carnitine and were dominated by complex II (site IIF in the reverse reaction). About half the rest was from site IF, but a significant portion, ~40 pmol H2O2 · min−1 · mg protein−1, was not from complex I, II, or III and was attributed to the proteins of β-oxidation (electron-transferring flavoprotein (ETF) and ETF-ubiquinone oxidoreductase). The maximum rate from the ETF system was ~200 pmol H2O2 · min−1 ~ mg protein−1 under conditions of compromised antioxidant defense and reduced ubiqui-none pool. Thus complex II and the ETF system both contribute to H2O2 production during fatty acid oxidation under appropriate conditions. PMID:23583329

The strategic significance of wastewater sources to pollutant phosphorus levels in English rivers and to environmental management for rural, agricultural and urban catchments.

PubMed

Neal, Colin; Jarvie, Helen P; Withers, Paul J A; Whitton, Brian A; Neal, Margaret

2010-03-01

The relationship between soluble and particulate phosphorus was examined for 9 major UK rivers including 26 major tributaries and 68 monitoring points, covering wide-ranging rural and agricultural/urban impacted systems with catchment areas varying from 1 to 6000km(2) scales. Phosphorus concentrations in Soluble Reactive (SRP), Total Dissolved (TDP), Total (TP), Dissolved Hydrolysable (DHP) and Particulate (PP) forms correlated with effluent markers (sodium and boron) and SRP was generally dominant signifying the importance of sewage sources. Low flows were particularly enriched in SRP, TDP and TP for average SRP>100microg/l indicating low effluent dilution. At particularly low average concentrations, SRP increased with flow but effluent sources were still implicated as the effluent markers (boron in particular) increased likewise. For rural areas, DHP had proportionately high concentrations and SRP+DHP concentrations could exceed environmental thresholds currently set for SRP. Given DHP has a high bioavailability the environmental implications need further consideration. PP concentrations were generally highest at high flows but PP in the suspended solids was generally at its lowest and in general PP correlated with particulate organic carbon and more so than the suspended sediment in total. Separation of pollutant inputs solely between effluent and diffuse (agriculture) components is misleading, as part of the "diffuse" term comprises effluents flushed from the catchments during high flow. Effluent sources of phosphorus supplied directly or indirectly to the river coupled with within-river interactions between water/sediment/biota largely determine pollutant levels. The study flags the fundamental need of placing direct and indirect effluent sources and contaminated storage with interchange to/from the river at the focus for remediation strategies for UK rivers in relation to eutrophication and the WFD.

Automated Power Assessment for Helicopter Turboshaft Engines

NASA Technical Reports Server (NTRS)

Simon, Donald L.; Litt, Jonathan S.

2008-01-01

An accurate indication of available power is required for helicopter mission planning purposes. Available power is currently estimated on U.S. Army Blackhawk helicopters by performing a Maximum Power Check (MPC), a manual procedure performed by maintenance pilots on a periodic basis. The MPC establishes Engine Torque Factor (ETF), an indication of available power. It is desirable to replace the current manual MPC procedure with an automated approach that will enable continuous real-time assessment of available power utilizing normal mission data. This report presents an automated power assessment approach which processes data currently collected within helicopter Health and Usage Monitoring System (HUMS) units. The overall approach consists of: 1) a steady-state data filter which identifies and extracts steady-state operating points within HUMS data sets; 2) engine performance curve trend monitoring and updating; and 3) automated ETF calculation. The algorithm is coded in MATLAB (The MathWorks, Inc.) and currently runs on a PC. Results from the application of this technique to HUMS mission data collected from UH-60L aircraft equipped with T700-GE-701C engines are presented and compared to manually calculated ETF values. Potential future enhancements are discussed.

Chemical and radiation crosslinked polymer electrolyte membranes prepared from radiation-grafted ETFE films for DMFC applications

NASA Astrophysics Data System (ADS)

Chen, Jinhua; Asano, Masaharu; Yamaki, Tetsuya; Yoshida, Masaru

To develop a highly chemically stable polymer electrolyte membrane for application in a direct methanol fuel cell (DMFC), doubly crosslinked membranes were prepared by chemical crosslinking using bifunctional monomers, such as divinylbenzene (DVB) and bis(p, p-vinyl phenyl) ethane (BVPE), and by radiation crosslinking. The membranes were prepared by grafting of m, p-methylstyrene (MeSt) and p-tert-butylstyrene (tBuSt) into poly(ethylene- co-tetrafluoroethylene) (ETFE) films and subsequent sulfonation. The effects of the DVB and BVPE crosslinkers on the grafting kinetics and the properties of the prepared membranes, such as water uptake, proton conductivity and chemical stability were investigated. Radiation crosslinking was introduced by irradiation of the ETFE base film, the grafted film or the sulfonated membrane. The membrane crosslinked by DVB and BVPE crosslinkers and post-crosslinked by γ-ray irradiation of the corresponding grafted film possessed the highest chemical stability among the prepared membranes, a significantly lower methanol permeability compared to Nafion ® membranes, and a better DMFC performance for high methanol feed concentration. Therefore, this doubly crosslinked membrane was promising for application in a DMFC where relatively high methanol concentration could be fed.

Structural organization and chromosomal assignment of the mouse embryonic TEA domain-containing factor (ETF) gene

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suzuki, Kazuo; Yasunami, Michio; Matsuda, Yoichi

1996-09-01

Embryonic TEA domain-containing factor (ETF) belongs to the family of proteins structurally related to transcriptional enhancer factor-1 (TEF-1) and is implicated in neural development. Isolation and characterization of the cosmid clones encoding the mouse ETF gene (Etdf) revealed that Etdf spans approximately 17.9 kb and consists of 12 exons. The exon-intron structure of Etdf closely resembles that of the Drosophila scalloped gene, indicating that these genes may have evolved from a common ancestor. Then multiple transcription initiation sites revealed by S1 protection and primer extension analyses are consistent with the absence of the canonical TATA and CAAT boxes in themore » 5{prime}-flanking region, which contains many potential regulatory sequences, such as the E-box, N-box, Sp1 element, GATA-1 element, TAATGARAT element, and B2 short interspersed element (SINE) as well as several direct and inverted repeat sequences. The Etdf locus was assigned to the proximal region of mouse chromosome 7 using fluorescence in situ hybridization and linkage mapping analyses. These results provide the molecular basis for studying the regulation, in vivo function, and evolution of Etdf. 29 refs., 5 figs., 1 tab.« less

Structural organization and chromosomal assignment of the mouse embryonic TEA domain-containing factor (ETF) gene.

PubMed

Suzuki, K; Yasunami, M; Matsuda, Y; Maeda, T; Kobayashi, H; Terasaki, H; Ohkubo, H

1996-09-01

Embryonic TEA domain-containing factor (ETF) belongs to the family of proteins structurally related to transcriptional enhancer factor-1 (TEF-1) and is implicated in neural development. Isolation and characterization of the cosmid clones encoding the mouse ETF gene (Etdf) revealed that Etdf spans approximately 17.9 kb and consists of 12 exons. The exon-intron structure of Etdf closely resembles that of the Drosophila scalloped gene, indicating that these genes may have evolved from a common ancestor. The multiple transcription initiation sites revealed by S1 protection and primer extension analyses are consistent with the absence of the canonical TATA and CAAT boxes in the 5'-flanking region, which contains many potential regulatory sequences, such as the E-box, N-box, Sp1 element, GATA-1 element, TAATGARAT element, and B2 short interspersed element (SINE) as well as several direct and inverted repeat sequences. The Etdf locus was assigned to the proximal region of mouse chromosome 7 using fluorescence in situ hybridization and linkage mapping analyses. These results provide the molecular basis for studying the regulation, in vivo function, and evolution of Etdf.

Does price efficiency increase with trading volume? Evidence of nonlinearity and power laws in ETFs

NASA Astrophysics Data System (ADS)

Caginalp, Gunduz; DeSantis, Mark

2017-02-01

Whether efficiency increases with increasing volume is an important issue that may illuminate trader strategies and distinguish between market theories. This relationship is tested using 124,236 daily observations comprising 68 large and liquid U.S. equity exchange traded funds (ETFs). ETFs have the advantage that efficiency can be measured in terms of the deviation between the trading price and the underlying net asset value that is reported each day. Our findings support the hypothesis that the relationship between volume and efficiency is nonlinear. Indeed, efficiency increases as volume increases from low to moderately high levels, but then decreases as volume increases further. The first part tends to support the idea that higher volume simply facilitates transactions and maintains efficiency, while the latter part, i.e., even higher volumes, supports the ansatz that increased volume is associated with increased speculation that ignores valuation and decreases efficiency. The results are consistent with the hypothesis that valuation is only part of the motivation for traders. Our methodology accounts for fund heterogeneity and contemporaneous correlations. Similar results are obtained when daily price volatility is introduced as an additional independent variable.

Relevance of emissions timing in biofuel greenhouse gases and climate impacts.

PubMed

Schwietzke, Stefan; Griffin, W Michael; Matthews, H Scott

2011-10-01

Employing life cycle greenhouse gas (GHG) emissions as a key performance metric in energy and environmental policy may underestimate actual climate change impacts. Emissions released early in the life cycle cause greater cumulative radiative forcing (CRF) over the next decades than later emissions. Some indicate that ignoring emissions timing in traditional biofuel GHG accounting overestimates the effectiveness of policies supporting corn ethanol by 10-90% due to early land use change (LUC) induced GHGs. We use an IPCC climate model to (1) estimate absolute CRF from U.S. corn ethanol and (2) quantify an emissions timing factor (ETF), which is masked in the traditional GHG accounting. In contrast to earlier analyses, ETF is only 2% (5%) over 100 (50) years of impacts. Emissions uncertainty itself (LUC, fuel production period) is 1-2 orders of magnitude higher, which dwarfs the timing effect. From a GHG accounting perspective, emissions timing adds little to our understanding of the climate impacts of biofuels. However, policy makers should recognize that ETF could significantly decrease corn ethanol's probability of meeting the 20% GHG reduction target in the 2007 Energy Independence and Security Act. The added uncertainty of potentially employing more complex emissions metrics is yet to be quantified.

Exercise in claudicants increase or decrease walking ability and the response relates to mitochondrial function.

PubMed

van Schaardenburgh, Michel; Wohlwend, Martin; Rognmo, Øivind; Mattsson, Erney J R

2017-06-07

Exercise of patients with intermittent claudication improves walking performance. Exercise does not usually increase blood flow, but seems to increase muscle mitochondrial enzyme activities. Although exercise is beneficial in most patients, it might be harmful in some. The mitochondrial response to exercise might therefore differ between patients. Our hypothesis was that changes in walking performance relate to changes in mitochondrial function after 8 weeks of exercise. At a subgroup level, negative responders decrease and positive responders increase mitochondrial capacity. Two types of exercise were studied, calf raising and walking (n = 28). We wanted to see whether there were negative and positive responders, independent of type of exercise. Measurements of walking performance, peripheral hemodynamics, mitochondrial respiration and content (citrate synthase activity) were obtained on each patient before and after the intervention period. Multiple linear regression was used to test whether changes in peak walking time relate to mitochondrial function. Subgroups of negative (n = 8) and positive responders (n = 8) were defined as those that either decreased or increased peak walking time following exercise. Paired t test and analysis of covariance was used to test changes within and between subgroups. Changes in peak walking time were related to changes in mitochondrial respiration supported by electron transferring flavoprotein (ETF + CI) P (p = 0.004), complex I (CI + ETF) P (p = 0.003), complex I + complex II (CI + CII + ETF) P (p = 0.037) and OXPHOS coupling efficiency (p = 0.046) in the whole group. Negative responders had more advanced peripheral arterial disease. Mitochondrial respiration supported by electron transferring flavoprotein (ETF + CI) P (p = 0.0013), complex I (CI + ETF) P (p = 0.0005), complex I + complex II (CI + CII + ETF) P (p = 0.011) and electron transfer system capacity (CI + CII + ETF) E (p = 0.021) and OXPHOS coupling efficiency decreased in negative responders (p = 0.0007) after exercise. Positive responders increased citrate synthase activity (p = 0.010). Changes in walking performance seem to relate to changes in mitochondrial function after exercise. Negative responders have more advanced peripheral arterial disease and decrease, while positive responders increase mitochondrial capacity. Trial registration ClinicalTrials.gov ID: NCT023110256.

A Novel Imaging Method for the Cartilaginous Eustachian Tube Lumen: Computerized Tomography During the Forced Response Test.

PubMed

Alper, Cuneyt M; Rath, Tanya J; Teixeira, Miriam S; Swarts, J Douglas

2018-01-01

In vivo imaging of the open cartilaginous Eustachian tube (ET) lumen by computed tomography (CT) scan during ET function (ETF) testing to establish new methodology. Five adults underwent unilateral ETF testing of an ear with a nonintact tympanic membrane using the forced response test (FRT) to measure the opening pressure (PO), steady state pressure (PS), and flow conductance (CS). Then at baseline and during the PS phase of the FRT, a temporal-bone CT scan with continuous 0.625 mm thickness was obtained. Multiplanar oblique reformats along the axis of the ET were created, and point value and region of interest (ROI) Hounsfield unit measurements were recorded from the location of the ET lumen. At the FRT flow rate of 11 ml/min, the average PO, PS, and CS were 370.5 daPa, 119.6 daPa, and 0.16 ml/min/daPa, respectively. For flow rates of 23 and 46 ml/min, these values were 236.2, 204.2, 0.12 and 385.5, 321.1, 0.18, respectively. Although areas with lower attenuation were suggestive of air density, a distinct air-filled cartilaginous ET lumen could not be confirmed. While the current imaging parameters failed to resolve the air-soft tissue interface throughout the open cartilaginous ET, further advances in imaging may obviate this limitation.

Geo-Accumulation Indices of Heavy Metals in Soil and Groundwater of Kanpur, India Under Long Term Irrigation of Tannery Effluent.

PubMed

Dotaniya, M L; Meena, V D; Rajendiran, S; Coumar, M Vassanda; Saha, J K; Kundu, S; Patra, A K

2017-05-01

Soil and groundwater from long-term (>50 years) tannery effluent irrigated areas of Kanpur were analyzed and significant buildup of heavy metals such as Cr, Ni, Cd, Pb, Zn, and As in the range of 252-972, 23-30, 2.3-14.1, 23.7-58.8, 138-338 and 6.8-11 mg kg -1 , respectively in soil was found. Few groundwater samples in the effluent irrigated areas also exhibited high Cr concentration above the permissible limit of United States Environmental Protection Agency. The tannery effluents contained 1.53-57.3 ppm Cr, 0-0.12 ppm Ni, 0-0.02 ppm Cd, 0-0.07 ppm Pb, 0-0.48 ppm Zn and 0-0.03 ppm As. The Geo-accumulation index (I geo ) revealed that soil samples were unpolluted to moderately polluted with Cu, Ni, Zn, Pb and As; moderately polluted in case of Cd; and heavily to extremely polluted by Cr.

Comparative analysis of effluent water quality from a municipal treatment plant and two on-site wastewater treatment systems.

PubMed

Garcia, Santos N; Clubbs, Rebekah L; Stanley, Jacob K; Scheffe, Brian; Yelderman, Joe C; Brooks, Bryan W

2013-06-01

Though decentralized on-site technologies are extensively employed for wastewater treatment around the globe, an understanding of effluent water quality impairments associated with these systems remain less understood than effluent discharges from centralized municipal wastewater treatment facilities. Using a unique experimental facility, a novel comparative analysis of effluent water quality was performed from model decentralized aerobic (ATS) and septic (STS) on-site wastewater treatment systems and a centralized municipal wastewater treatment plant (MTP). The ATS and STS units did not benefit from further soil treatment. Each system received common influent wastewater from the Waco, Texas, USA Metropolitan Area Regional Sewerage System. We tested the hypothesis that MTP effluent would exhibit higher water quality than on-site effluents, based on parameters selected for study. A tiered testing approach was employed to assess the three effluent discharges: select routine water quality parameters (Tier I), whole effluent toxicity (Tier II), and select endocrine-active compounds (Tier III). Contrary to our hypothesis, ATS effluent was not statistically different from MTP effluents, based on Tier I and III parameters, but reproductive responses of Daphnia magna were slightly more sensitive to ATS than MTP effluents. STS effluent water quality was identified as most degraded of the three wastewater treatment systems. Parameters used to assess centralized wastewater treatment plant effluent water quality such as whole effluent toxicity and endocrine active substances appear useful for water quality assessments of decentralized discharges. Aerobic on-site wastewater treatment systems may represent more robust options than traditional septic systems for on-site wastewater treatment in watersheds with appreciable groundwater - surface water exchange. Copyright © 2013 Elsevier Ltd. All rights reserved.

An evaluation of the application of treated sewage effluents in Las Tablas de Daimiel National Park, Central Spain

NASA Astrophysics Data System (ADS)

Navarro, Vicente; García, Beatriz; Sánchez, David; Asensio, Laura

2011-04-01

SummaryAt the present time there is not enough information available to develop a quantitative model on how inundation takes place in the 1490 ha area of Tablas de Daimiel National Park (Central Spain) located upstream of Morenillo Dam. Given that it is the most important area in the Park from an ecological standpoint, this is a major concern, as it has not been possible to assess the potential effectiveness of the interventions geared towards improving its current state. As a result, it is not feasible to simulate the hydrologic response to the application of treated sewage effluents, an initiative recently implemented by the Public Administration responsible for water management in the Guadiana River Basin, where the Park is located. To help solve this problem, a simplified model of the hydrologic behaviour of the system has been developed focusing on the characterisation of the main trends of the inundation process. Field data from 12 drying processes were used to identify the model parameters. Later, the evolution of the system was examined after the application of treated sewage effluents, assuming the hypothesis of a dry climate. The results show that the 10 Mm 3 of available effluents is sufficient to improve from 2 ha to 60 ha the inundation condition of the areas considered to be high-priority. This therefore demonstrates that, from a hydrologic point of view, it is highly advisable to use treated sewage effluents.

40 CFR 434.52 - Effluent limitations quidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2013 CFR

2013-07-01

... technology currently available (BPT). 434.52 Section 434.52 Protection of Environment ENVIRONMENTAL... practicable control technology currently available (BPT). (a) Reclamation areas. The limitations in this... this subsection after application of the best practicable control technology currently available: BPT...

40 CFR 434.52 - Effluent limitations quidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2014 CFR

2014-07-01

... technology currently available (BPT). 434.52 Section 434.52 Protection of Environment ENVIRONMENTAL... practicable control technology currently available (BPT). (a) Reclamation areas. The limitations in this... this subsection after application of the best practicable control technology currently available: BPT...

40 CFR 434.52 - Effluent limitations quidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

... technology currently available (BPT). 434.52 Section 434.52 Protection of Environment ENVIRONMENTAL... practicable control technology currently available (BPT). (a) Reclamation areas. The limitations in this... this subsection after application of the best practicable control technology currently available: BPT...

40 CFR 434.52 - Effluent limitations quidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2012 CFR

2012-07-01

... technology currently available (BPT). 434.52 Section 434.52 Protection of Environment ENVIRONMENTAL... practicable control technology currently available (BPT). (a) Reclamation areas. The limitations in this... this subsection after application of the best practicable control technology currently available: BPT...

40 CFR 434.52 - Effluent limitations quidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

... technology currently available (BPT). 434.52 Section 434.52 Protection of Environment ENVIRONMENTAL... practicable control technology currently available (BPT). (a) Reclamation areas. The limitations in this... this subsection after application of the best practicable control technology currently available: BPT...

OCCURRENCE OF ANTIBIOTIC-RESISTANT UROPATHOGENIC ESCHERICHIA COLI CLONAL GROUP A IN WASTEWATER EFFLUENTS

EPA Science Inventory

Isolates of Escherichia coli belonging to clonal group A (CGA), a recently described disseminated cause of drug-resistant urinary tract infections in humans, were present in four of seven sewage effluents collected from geographically dispersed areas of the United States. ...

40 CFR 412.46 - New source performance standards (NSPS).

Code of Federal Regulations, 2010 CFR

2010-07-01

... production areas. There must be no discharge of manure, litter, or process wastewater pollutants into waters... practice effluent limitations designed to ensure no discharge of manure, litter, or process wastewater... such effluent limitations, “no discharge of manure, litter, or process wastewater pollutants,” as used...

Determination of Phenols and Trimethylamine in Industrial Effluents

NASA Technical Reports Server (NTRS)

Levaggi, D. A.; Feldstein, M.

1971-01-01

For regulatory purposes to control certain odorous compounds the analysis of phenols and trimethylamines in industrial effluents is necessary. The Bay Area Air Pollution Control District laboratory has been determining these gases by gas chromatographic techniques. The procedures for sample collection, preparation for analysis and determination are described in detail. Typical data from various sources showing the effect of proposed regulations is shown. Extensive sampling and usage of these procedures has shown them to be accurate, reliable and suitable to all types of source effluents.

Groundwater pollution around an industrial area in the coastal stretch of Maharashtra State, India.

PubMed

Naik, Pradeep K; Dehury, Biranchi N; Tiwari, Arun N

2007-09-01

The main objective of this paper is to examine pollution threat, especially to the groundwater resources, around Tarapur industrial area (also called the Tarapur MIDC area) located on the Arabian Sea Coast in Thane District of Maharashtra State, India and suggest remedial measures that may also be relevant to other industrial areas on the Indian Sea Coast. One hundred and thirty one samples were collected from various sources, such as dugwells, borewells, dug-cum-borewells, effluent sumps, drainage channels (effluent channels), creeks and ocean, for chemical analyses. These analyses show that the area in general is characterized by hard water and high salinity hazard, possibly due to its proximity and hydraulic connection with the sea. Although the potability of groundwater is questionable in certain pockets, it is good enough for irrigation purposes at present. Low pH value and high heavy metal contents in the adjoining Muramba creek water is a matter of great concern and may be attributed to the indiscriminate disposal of industrial effluents to the drainage channels connecting the creek. Muramba Creek is well connected with the Arabian Sea, and there are evidences of seawater intrusion around this creek. Because of the fact that Muramba Creek is highly polluted, and is hydraulically connected with the dugwells and borewells surrounding the creek, it cannot be ruled out that the groundwater around this creek is susceptible to contamination. Unless measures are not taken immediately to stop the indiscriminate disposal of the solid wastes and liquid effluents in open ground and drainage channels, and measures are not taken to maintain the appropriate pH values at the effluent treatment facilities before their disposal, the problem would indeed be formidable one day, and it will be too late then for the authorities to take care of the resulting maladies. Few suggestions have been given for controlling and managing the industrial pollution around the Tarapur MIDC area. These suggestions are relevant to other industrial areas situated on the 7,000 km long Indian Sea Coast.

Wastewater Treatment Effluent Reduces the Abundance and Diversity of Benthic Bacterial Communities in Urban and Suburban Rivers

PubMed Central

Drury, Bradley; Rosi-Marshall, Emma

2013-01-01

In highly urbanized areas, wastewater treatment plant (WWTP) effluent can represent a significant component of freshwater ecosystems. As it is impossible for the composition of WWTP effluent to match the composition of the receiving system, the potential exists for effluent to significantly impact the chemical and biological characteristics of the receiving ecosystem. We assessed the impacts of WWTP effluent on the size, activity, and composition of benthic microbial communities by comparing two distinct field sites in the Chicago metropolitan region: a highly urbanized river receiving effluent from a large WWTP and a suburban river receiving effluent from a much smaller WWTP. At sites upstream of effluent input, the urban and suburban rivers differed significantly in chemical characteristics and in the composition of their sediment bacterial communities. Although effluent resulted in significant increases in inorganic nutrients in both rivers, surprisingly, it also resulted in significant decreases in the population size and diversity of sediment bacterial communities. Tag pyrosequencing of bacterial 16S rRNA genes revealed significant effects of effluent on sediment bacterial community composition in both rivers, including decreases in abundances of Deltaproteobacteria, Desulfococcus, Dechloromonas, and Chloroflexi sequences and increases in abundances of Nitrospirae and Sphingobacteriales sequences. The overall effect of the WWTP inputs was that the two rivers, which were distinct in chemical and biological properties upstream of the WWTPs, were almost indistinguishable downstream. These results suggest that WWTP effluent has the potential to reduce the natural variability that exists among river ecosystems and indicate that WWTP effluent may contribute to biotic homogenization. PMID:23315724

Technical potential of microalgal bacterial floc raceway ponds treating food-industry effluents while producing microalgal bacterial biomass: An outdoor pilot-scale study.

PubMed

Van Den Hende, Sofie; Beelen, Veerle; Julien, Lucie; Lefoulon, Alexandra; Vanhoucke, Thomas; Coolsaet, Carlos; Sonnenholzner, Stanislaus; Vervaeren, Han; Rousseau, Diederik P L

2016-10-01

To replace costly mechanical aeration by photosynthetical aeration, upflow anaerobic sludge blanket (UASB) effluent of food-industry was treated in an outdoor MaB-floc raceway pond. Photosynthetic aeration was sufficient for nitrification, but the raceway effluent quality was below current discharge limits, despite the high hydraulic retention time (HRT) of 35days. Hereafter, conventional activated sludge (CAS) effluent of food-industry was treated in this pond to recover phosphorus. The two-day HRT results in a more realistic pond area, but the phosphorus removal efficiency was low (20%). High biomass productivities were obtained, i.e. 31.3 and 24.9ton total suspended solids hapond(-1)year(-1) for UASB and CAS effluent, respectively. Bioflocculation enabled successful harvesting of CAS effluent-fed MaB-flocs by settling and filtering at 150-250μm to 22.7% total solids. To conclude, MaB-floc raceway ponds cannot be recommended as the sole treatment for these food-industry effluents, but huge potential lies in added-value biomass production. Copyright © 2016 Elsevier Ltd. All rights reserved.

Millimeter wave sensor for monitoring effluents

DOEpatents

Gopalsami, Nachappa; Bakhtiari, Sasan; Raptis, Apostolos C.; Dieckman, Stephen L.

1995-01-01

A millimeter-wave sensor for detecting and measuring effluents from processing plants either remotely or on-site includes a high frequency signal source for transmitting frequency-modulated continuous waves in the millimeter or submillimeter range with a wide sweep capability and a computer-controlled detector for detecting a plurality of species of effluents on a real time basis. A high resolution spectrum of an effluent, or effluents, is generated by a deconvolution of the measured spectra resulting in a narrowing of the line widths by 2 or 3 orders of magnitude as compared with the pressure broadened spectra detected at atmospheric pressure for improved spectral specificity and measurement sensitivity. The sensor is particularly adapted for remote monitoring such as where access is limited or sensor cost restricts multiple sensors as well as for large area monitoring under nearly all weather conditions.

77 FR 29167 - Effluent Limitations Guidelines and New Source Performance Standards for the Airport Deicing...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-16

... reductions in dissolved oxygen, fish kills, reduced organism abundance and species diversity, contamination... 13045: Protection of Children From Environmental Health and Safety Risks H. EO 13211: Energy Effects I... water in residential areas and parkland, and other effects. Today, EPA is promulgating effluent...

Epidemiological studies on Salmonella in a certain area ("Walcheren project"). I. The presence of Salmonella in man, pigs, insects, seagulls and in foods and effluents.

PubMed

Edel, W; van Schothorst, M; Kampelmacher, E H

1976-08-01

During a certain period various materials (pigs, foods, insects, seagull droppings, chopping-block scrapings from butcher's shops, effluents of sewage treatment plants and stools of patients) were examined for the presence of Salmonella at the same time in a relatively small area (Walcheren). Certain types of Salmonella (S. typhi murium type II 505, S. panama, S. infantis and S. brandenburg) were frequently isolated from almost all materials examined. This may indicate the existence of Salmonella contamination cycles: one may think of the cycle: slaughter animal (infected from the environment and/or by meal) - meat - consumer - patient or healthy carrier - effluent and surface water - insects, birds and rodents - slaughter animal or meat and possibly other foods - consumer.

Immunocompetence analysis of the aquatic snail Lymnaea stagnalis exposed to urban wastewaters.

PubMed

Boisseaux, Paul; Noury, Patrice; Delorme, Nicolas; Perrier, Lucile; Thomas-Guyon, Helene; Garric, Jeanne

2018-04-02

Wastewater treatment plant effluents from urban area are a well-known source of chronic multiple micropollution to the downstream living organisms. In this study, ecologically relevant laboratory-bred freshwater gastropods, Lymnaea stagnalis, were exposed for 29 days to raw effluents of a wastewater treatment plant in Lyon area (France). A time-course analysis of individual markers of immunocompetence (hemocyte density and viability, hemocyte NADPH activity, phenol oxidase activity, and capacity of phagocytosis) has shown slight trends of inflammatory-like responses induced by the 100% effluents. So far, no short-term hazard for L. stagnalis can be revealed. However, over the long term, such environmental stress-stimulating immune responses could provoke deleterious life history trade-offs because the immune system is known to be highly energy-consuming.

Wastewater movement near four treatment and disposal sites in Yellowstone National Park, Wyoming

USGS Publications Warehouse

Cox, E.R.

1986-01-01

The U.S. Geological Survey, in cooperation with the National Park Service, studied the effects on nearby streams and lakes of treated wastewater effluents that percolate from sewage lagoons at four sites in Yellowstone National Park. A network of observation wells has been established near the sites, and water level and water quality data were collected from 1974 through 1982. Groundwater mounds occur under the lagoons as percolation of effluents occurs. The percolating effluents mix with groundwater and form plumes of water that contain chemical constituents from the effluents. These plumes move down the hydraulic gradient toward groundwater discharge areas. The directions of movement of percolating effluents have been determined by analyzing water samples from wells near the lagoons for specific conductance, chloride concentration, and nitrite plus nitrate concentration. Other constituents and properties also were determined. The percolating effluents are diluted by groundwater and have no discernible effects on the quality of water in the nearby streams and lakes. (USGS)

Hydrogeology and Migration of Septic-Tank Effluent in the Surficial Aquifer System in the Northern Midlands Area, Palm Beach County, Florida

USGS Publications Warehouse

Miller, Wesley L.

1992-01-01

The northern Midlands area in Palm Beach County is an area of expected residential growth, but its flat topography, poor drainage, and near-surface marl layers retard rainfall infiltration and cause frequent flooding. Public water supplies and sewer services are not planned for the area, thus, residents must rely on domestic wells and septic tanks. The water table in the northern Midlands area is seldom more than 5 feet below land surface, and regional ground-water flows are east, southwest, and south from the north-central part of the area where ground-water levels are highest. Ground-water quality in the western part of the area and in the Loxahatchee Slough is greatly influenced by residual seawater emplaced during the Pleistocene Epoch. Chloride and dissolved-solids concentrations of ground water in the surficial aquifer system in these areas often exceed secondary drinking-water standards. Residual seawater has been more effectively flushed from the more permeable sediments elsewhere in the eastern and southwestern parts of the study area. Test at three septic-tank sites showed traces of effluent in ground water (38-92 feet from the septic tank outlets) and that near-surface marl layers greatly impede the downward migration of the effluent in the surficial aquifer system throughout the northern midlands.

Silage effluent management: a review.

PubMed

Gebrehanna, M M; Gordon, R J; Madani, A; VanderZaag, A C; Wood, J D

2014-10-01

Silage effluent is a potent wastewater that can be produced when ensiling crops that have a high moisture content (MC). Silage effluent can cause fish-kills and eutrophication due to its high biochemical oxygen demand (BOD) and nutrient content, respectively. It has a high acidity (pH ≈ 3.5-5) making it corrosive to steel and damaging to concrete, which makes handling, storage and disposal a challenge. Although being recognized as a concentrated wastewater, most research has focused on preventing its production. Despite noted imprecision in effluent production models-and therefore limited ability to predict when effluent will flow-there has been little research aimed at identifying effective reactive management options, such as containment and natural treatment systems. Increasing climate variability and intensifying livestock agriculture are issues that will place a greater importance on developing comprehensive, multi-layered management strategies that include both preventative and reactive measures. This paper reviews important factors governing the production of effluent, approaches to minimize effluent flows as well as treatment and disposal options. The challenges of managing silage effluent are reviewed in the context of its chemical constituents. A multi-faceted approach should be utilized to minimize environmental risks associated with silage effluent. This includes: (i) managing crop moisture content prior to ensiling to reduce effluent production, (ii) ensuring the integrity of silos and effluent storages, and (iii) establishing infrastructure for effluent treatment and disposal. A more thorough investigation of constructed wetlands and vegetated infiltration areas for treating dilute silage effluent is needed. In particular, there should be efforts to improve natural treatment system design criteria by identifying pre-treatment processes and appropriate effluent loading rates. There is also a need for research aimed at understanding the effects of repeated land application of effluent on soil quality and crop yields, as spreading is a common disposal practice. Copyright © 2014 Elsevier Ltd. All rights reserved.

Occurrence of betablockers in effluents of wastewater treatment plants from the Lyon area (France) and risk assessment for the downstream rivers.

PubMed

Miège, Cécile; Favier, Maxime; Brosse, Corinne; Canler, Jean-Pierre; Coquery, Marina

2006-11-15

Five betablockers (oxprenolol, metoprolol, propranolol, bisoprolol, betaxolol) were analysed in effluents collected over a 3-month period from wastewater treatment plants (WTP) from the Lyon area in France. The analytical protocol consisted of solid phase extraction of the dissolved aqueous phase on HLB cartridges and analysis by gas chromatography coupled with mass detection (GC-MS) after derivatization. Concentrations of metoprolol, propranolol and bisoprolol varied from 45 to 2838ng/L whereas oxprenolol and betaxolol were never detected in these effluent samples. A high variability of betablockers concentrations and fluxes was observed between WTP effluents and within each WTP over the time period studied. Considering a flux per person for a dry weather period, Fontaine plant was pointed out as the less efficient WTP, which might be explained by its type of treatment (biological aerated filters). But we need additional analysis of effluent and influent waters to confirm this hypothesis. A tentative approach of local environmental risk assessment of propranolol based on the calculation of PEC/PNEC (predicted environmental concentration/predicted non effect concentration) ratio approach lead us to conclude on a negligible risk for the downstream rivers (Rhône river at Ternay and Saône river at Couzon Mt d'Or).

Selective material ablation by the TEA CO2 laser

NASA Astrophysics Data System (ADS)

Sumiyoshi, Tetsumi; Shiratori, Akira; Ninomiya, Yutaka; Obara, Minoru

1995-03-01

This paper reports two topics in the material processing using TEA CO2 lasers. We demonstrated selective ablation of hydrogenated amorphous silicon (a-Si:H) thin layer on a quartz substrate by the second harmonic (SH) radiation of TEA CO2 laser generated by AgGaSe2 nonlinear crystal. Si-H bonds contained in a-Si:H strongly absorb the 5 micrometers SH radiation and resulted in the selective ablation of the a-Si:H layer. The successful ablation processing of ethylenetetrafluoroethylene (ETFE) copolymer by the 9.6 micrometers fundamental wavelength TEA CO2 laser is also reported. Only ETFE thin film adhered to an aluminum substrate can be ablated by the TEA CO2 laser.

Investigation of grafted ETFE-based polymer membranes as alternative electrolyte for direct methanol fuel cells

NASA Astrophysics Data System (ADS)

Aricò, A. S.; Baglio, V.; Cretı̀, P.; Di Blasi, A.; Antonucci, V.; Brunea, J.; Chapotot, A.; Bozzi, A.; Schoemans, J.

Low cost ethylene-tetrafluoroethylene (ETFE)-based grafted membranes have been prepared by a process based on electron beam irradiation, subsequent grafting, cross-linking and sulfonation procedure. Two different grafted membranes varying by their grafting and cross-linking levels have been investigated for applications in direct methanol fuel cells (DMFCs) operating between 90 and 130 °C. DMFC assemblies based on these membranes showed cell resistance and performance values comparable to Nafion 117. Stable electrochemical performance was recorded during 1 month of cycled operation. Tailoring of grafting and cross-linking properties allows a significant reduction of methanol cross-over while maintaining suitable conductivity and performance levels.

Surface effects in the unitary Fermi gas

NASA Astrophysics Data System (ADS)

Salasnich, L.; Ancilotto, F.; Toigo, F.

2010-01-01

We study the extended Thomas-Fermi (ETF) density functional of the superfluid unitary Fermi gas. This functional includes a gradient term which is essential to describe accurately the surface effects of the system, in particular with a small number of atoms, where the Thomas-Fermi (local density) approximation fails. We find that our ETF functional gives density profiles which are in good agreement with recent Monte Carlo results and also with a more sophisticated superfluid density functional based on Bogoliubov-de Gennes equations. In addition, by using extended hydrodynamics equations of superfluids, we calculate the frequencies of collective surface oscillations of the unitary Fermi gas, showing that quadrupole and octupole modes strongly depend on the number of trapped atoms.

Cross-correlation analysis between Chinese TF contracts and treasury ETF based on high-frequency data

NASA Astrophysics Data System (ADS)

Zhou, Yu; Chen, Shi

2016-02-01

In this paper, we investigate the high-frequency cross-correlation relationship between Chinese treasury futures contracts and treasury ETF. We analyze the logarithmic return of these two price series, from which we can conclude that both return series are not normally distributed and the futures markets have greater volatility. We find significant cross-correlation between these two series. We further confirm the relationship using the DCCA coefficient and the DMCA coefficient. We quantify the long-range cross-correlation with DCCA method, and we further show that the relationship is multifractal. An arbitrage algorithm based on DFA regression with stable return is proposed in the last part.

Engineering test facility design definition

NASA Technical Reports Server (NTRS)

Bercaw, R. W.; Seikel, G. R.

1980-01-01

The Engineering Test Facility (ETF) is the major focus of the Department of Energy (DOE) Magnetohydrodynamics (MHD) Program to facilitate commercialization and to demonstrate the commercial operability of MHD/steam electric power. The ETF will be a fully integrated commercial prototype MHD power plant with a nominal output of 200 MW sub e. Performance of this plant is expected to meet or surpass existing utility standards for fuel, maintenance, and operating costs; plant availability; load following; safety; and durability. It is expected to meet all applicable environmental regulations. The current design concept conforming to the general definition, the basis for its selection, and the process which will be followed in further defining and updating the conceptual design.

Artificial Sweeteners Reveal Septic System Effluent in Rural Groundwater.

PubMed

Spoelstra, John; Senger, Natalie D; Schiff, Sherry L

2017-11-01

It has been widely documented that municipal wastewater treatment plant effluents are a major source of artificial sweeteners to surface waters. However, in rural areas, the extent to which septic systems contribute these same compounds to groundwater aquifers is largely unknown. We examined the occurrence of four commonly used artificial sweeteners in an unconfined sand aquifer that serves as a water supply for rural residents, as a receptor of domestic wastewater from septic systems, and as a source of baseflow to the Nottawasaga River, ON, Canada. Groundwater from the Lake Algonquin Sand Aquifer in the southern Nottawasaga River Watershed was collected from private domestic wells and as groundwater seeps discharging along the banks of the Nottawasaga River. Approximately 30% of samples had detectable levels of one or more artificial sweeteners, indicating the presence of water derived from septic system effluent. Using acesulfame concentrations to estimate the fraction of septic effluent in groundwater samples, ∼3.4 to 13.6% of the domestic wells had 1% or more of their well water being derived from septic system effluent. Similarly, 2.0 to 4.7% of the groundwater seeps had a septic effluent contribution of 1% or more. No relationship was found between the concentration of acesulfame and the concentration of nitrate, ammonium, or soluble reactive phosphorus in the groundwater, indicating that septic effluent is not the dominant source of nutrients in the aquifer. It is expected that the occurrence of artificial sweeteners in shallow groundwater is widespread throughout rural areas in Canada. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

Paper plant effluent revisited-southern Lake Champlain, Vermont and New York

USGS Publications Warehouse

Haupt, R.S.; Folger, D.W.

1993-01-01

We used geologic and geochemical techniques to document the change with time of the distribution and concentration of contaminated bottom sediments in southern Lake Champlain near an International Paper Company plant. Our work, initiated in 1972, was expanded on behalf of Vermont citizens in a class-action suit against the International Paper Company. To update our 1972-1973 results, we collected nine cores in 1988 upstream and downstream from the paper plant effluent diffuser. Water content, volatile solids, organic carbon, and three ratios, Al/Si, Cl/Si, and S/Si, in addition to megascopic and microscopic observations, were evaluated to identify and trace the distribution of effluent and to measure the thickness of sediment affected by or containing components of effluent. Analyses were carried out on samples from the cores as well as from effluent collected directly from the plant's waste treatment facility. In 1973, two years after the plant opened, we cored near the diffuser; sediment contaminated with effluent was 4.5 cm thick. In 1988, in the same area, sediment contaminated with effluent was 17 cm thick. In 15 years, water content increased from 72 to 85 percent, volatile solids from 7 to 20 percent, and organic carbon from 2 to 12 percent. Cl/Si and S/Si were high only near the diffuser and were zero elsewhere. In the area of the diffuser, contaminated sediment appears to be accumulating at a rate of about 1 cm/yr. At a control location 22 km upstream (south) from the plant, the top, poorly consoli-dated layer was only 1 cm or less thick both in 1973 and in 1988. The class-action suit was settled in favor of the plaintiffs for $5 million. ?? 1993 Springer-Verlag.

Mercury in ground water, septage, leach-field effluent, and soils in residential areas, New Jersey coastal plain

USGS Publications Warehouse

Barringer, J.L.; Szabo, Z.; Schneider, D.; Atkinson, W.D.; Gallagher, R.A.

2006-01-01

Water samples were collected from domestic wells at an unsewered residential area in Gloucester County, New Jersey where mercury (Hg) concentrations in well water were known to exceed the USEPA maximum contaminant level (MCL) of 2000 ng/L. This residential area (the CSL site) is representative of more than 70 such areas in southern New Jersey where about 600 domestic wells, sampled previously by State and county agencies, yielded water containing Hg at concentrations that exceed the MCL. Recent studies indicate that background concentrations of Hg in water from this unconfined sand and gravel aquifer system are < 10 ng/L. Additional sampling was conducted at the CSL site in order to better understand sources of Hg and potential Hg transport mechanisms in the areas with Hg-contaminated ground water. At the CSL site, concentrations of Hg were substantially lower (although still exceeding the MCL in some cases) in filtered water samples than in the unfiltered water samples collected previously from the same wells. Surfactants and elevated concentrations of sodium, chloride, nitrate, ammonium, and phosphate in water from domestic and observation wells indicated septic-system effects on water quality; detections of sulfide indicated localized reducing conditions. Hg concentrations in septage and leach-field effluent sampled at several other households in the region were low relative to the contaminant-level Hg concentrations in water from domestic wells. Relations of Hg concentrations in leach-field effluent to iron concentrations indicate that reductive dissolution of iron hydroxides in soils may release Hg to the percolating effluent. ?? 2005 Elsevier B.V. All rights reserved.

Bio-filtration capacity, oxygen consumption and ammonium excretion of Dosinia ponderosa and Chione gnidia (Veneroida: Veneridae) from areas impacted and non-impacted by shrimp aquaculture effluents.

PubMed

Ramos-Corella, Karime; Martínez-Córdova, Luis Rafael; Enríquez-Ocaña, Luis Fernando; Miranda-Baeza, Anselmo; López-Elías, José Antonio

2014-09-01

Mollusks are some of the most important, abundant and diverse organisms inhabiting not only aquatic ecosystems, but also terrestrial environments. Recently, they have been used for bioremediation of aquaculture effluents; nevertheless, for that purpose it is necessary to analyze the capacity of a particular species. In this context, an experimental investigation was developed to evaluate the performance of two bivalves C. gnidia and D. ponderosa, collected from areas with or without shrimp aquaculture effluents. For this, the filtration capacity (as clearance rate) as well as the oxygen consumption and ammonia excretion rates were measured following standard methods. The clearance rate was significantly higher for D. ponderosa from impacted areas, when com- pared to C. gnidia, from both areas. Contrarily, the oxygen consumption was greater for C. gnidia from impacted areas compared to D. ponderosa from both areas. The same tendency was observed for the ammonia excretion with the highest rates observed for C. gnidia from impacted areas, whereas no differences were observed among D. ponderosa from both areas. The results suggest that both species developed different strategies to thrive and survive under the impacted conditions; D. ponderosa improved its filtration efficiency, while C. gnidia modified its oxygen consumption and ammonia excretion. We concluded that both species, and particularly D. ponderosa, can be used for bioremediation purposes.

Multiple Acyl-CoA Dehydrogenation Deficiency (Glutaric Aciduria Type II) with a Novel Mutation of Electron Transfer Flavoprotein-Dehydrogenase in a Cat.

PubMed

Wakitani, Shoichi; Torisu, Shidow; Yoshino, Taiki; Hattanda, Kazuhisa; Yamato, Osamu; Tasaki, Ryuji; Fujita, Haruo; Nishino, Koichiro

2014-01-01

Multiple acyl-CoA dehydrogenation deficiency (MADD; also known as glutaric aciduria type II) is a human autosomal recessive disease classified as one of the mitochondrial fatty-acid oxidation disorders. MADD is caused by a defect in the electron transfer flavoprotein (ETF) or ETF dehydrogenase (ETFDH) molecule, but as yet, inherited MADD has not been reported in animals. Here we present the first report of MADD in a cat. The affected animal presented with symptoms characteristic of MADD including hypoglycemia, hyperammonemia, vomiting, diagnostic organic aciduria, and accumulation of medium- and long-chain fatty acids in plasma. Treatment with riboflavin and L-carnitine ameliorated the symptoms. To detect the gene mutation responsible for MADD in this case, we determined the complete cDNA sequences of feline ETFα, ETFβ, and ETFDH. Finally, we identified the feline patient-specific mutation, c.692T>G (p.F231C) in ETFDH. The affected animal only carries mutant alleles of ETFDH. p.F231 in feline ETFDH is completely conserved in eukaryotes, and is located on the apical surface of ETFDH, receiving electrons from ETF. This study thus identified the mutation strongly suspected to have been the cause of MADD in this cat.

Development of an automation technique for the establishment of functional lipid bilayer arrays

NASA Astrophysics Data System (ADS)

Hansen, J. S.; Perry, M.; Vogel, J.; Vissing, T.; Hansen, C. R.; Geschke, O.; Emnéus, J.; Nielsen, C. H.

2009-02-01

In the present work, a technique for establishing multiple black lipid membranes (BLMs) in arrays of micro structured ethylene tetrafluoroethylene (ETFE) films, and supported by a micro porous material was developed. Rectangular 8 × 8 arrays with apertures having diameters of 301 ± 5 µm were fabricated in ETFE Teflon film by laser ablation using a carbon dioxide laser. Multiple lipid membranes could be formed across the micro structured 8 × 8 array ETFE partitions. Success rates for the establishment of cellulose-supported BLMs across the multiple aperture arrays were above 95%. However, the time course of the membrane thinning process was found to vary considerably between multiple aperture bilayer experiments. An airbrush partition pretreatment technique was developed to increase the reproducibility of the multiple lipid bilayers formation during the time course from the establishment of the lipid membranes to the formation of bilayers. The results showed that multiple lipid bilayers could be reproducible formed across the airbrush-pretreated 8 × 8 rectangular arrays. The ionophoric peptide valinomycin was incorporated into established membrane arrays, resulting in ionic currents that could be effectively blocked by tetraethylammonium. This shows that functional bimolecular lipid membranes were established, and furthermore outlines that the established lipid membrane arrays could host functional membrane-spanning molecules.

An Evaluation of the Performance and Economics of Membranes and Separators in Single Chamber Microbial Fuel Cells Treating Domestic Wastewater.

PubMed

Christgen, Beate; Scott, Keith; Dolfing, Jan; Head, Ian M; Curtis, Thomas P

2015-01-01

The cost of materials is one of the biggest barriers for wastewater driven microbial fuel cells (MFCs). Many studies use expensive materials with idealistic wastes. Realistically the choice of an ion selective membrane or nonspecific separators must be made in the context of the cost and performance of materials available. Fourteen membranes and separators were characterized for durability, oxygen diffusion and ionic resistance to enable informed membrane selection for reactor tests. Subsequently MFCs were operated in a cost efficient reactor design using Nafion, ethylene tetrafluoroethylene (ETFE) or polyvinylidene fluoride (PVDF) membranes, a nonspecific separator (Rhinohide), and a no-membrane design with a carbon-paper internal gas diffusion cathode. Peak power densities during polarisation, from MFCs using no-membrane, Nafion and ETFE, reached 67, 61 and 59 mWm(-2), and coulombic efficiencies of 68±11%, 71±12% and 92±6%, respectively. Under 1000 Ω, Nafion and ETFE achieved an average power density of 29 mWm(-2) compared to 24 mWm(-2) for the membrane-less reactors. Over a hypothetical lifetime of 10 years the generated energy (1 to 2.5 kWhm(-2)) would not be sufficient to offset the costs of any membrane and separator tested.

Development and evaluation of a home enteral nutrition team.

PubMed

Dinenage, Sarah; Gower, Morwenna; Van Wyk, Joanna; Blamey, Anne; Ashbolt, Karen; Sutcliffe, Michelle; Green, Sue M

2015-03-05

The organisation of services to support the increasing number of people receiving enteral tube feeding (ETF) at home varies across regions. There is evidence that multi-disciplinary primary care teams focussed on home enteral nutrition (HEN) can provide cost-effective care. This paper describes the development and evaluation of a HEN Team in one UK city. A HEN Team comprising dietetians, nurses and a speech and language therapist was developed with the aim of delivering a quality service for people with gastrostomy tubes living at home. Team objectives were set and an underpinning framework of organisation developed including a care pathway and a schedule of training. Impact on patient outcomes was assessed in a pre-post test evaluation design. Patients and carers reported improved support in managing their ETF. Cost savings were realised through: (1) prevention of hospital admission and related transport for ETF related issues; (2) effective management and reduction of waste of feed and thickener; (3) balloon gastrostomy tube replacement by the HEN Team in the patient's home, and optimisation of nutritional status. This service evaluation demonstrated that the establishment of a dedicated multi-professional HEN Team focussed on achievement of key objectives improved patient experience and, although calculation of cost savings were estimates, provided evidence of cost-effectiveness.

An Evaluation of the Performance and Economics of Membranes and Separators in Single Chamber Microbial Fuel Cells Treating Domestic Wastewater

PubMed Central

Christgen, Beate; Scott, Keith; Dolfing, Jan; Head, Ian M.; Curtis, Thomas P.

2015-01-01

The cost of materials is one of the biggest barriers for wastewater driven microbial fuel cells (MFCs). Many studies use expensive materials with idealistic wastes. Realistically the choice of an ion selective membrane or nonspecific separators must be made in the context of the cost and performance of materials available. Fourteen membranes and separators were characterized for durability, oxygen diffusion and ionic resistance to enable informed membrane selection for reactor tests. Subsequently MFCs were operated in a cost efficient reactor design using Nafion, ethylene tetrafluoroethylene (ETFE) or polyvinylidene fluoride (PVDF) membranes, a nonspecific separator (Rhinohide), and a no-membrane design with a carbon-paper internal gas diffusion cathode. Peak power densities during polarisation, from MFCs using no-membrane, Nafion and ETFE, reached 67, 61 and 59 mWm-2, and coulombic efficiencies of 68±11%, 71±12% and 92±6%, respectively. Under 1000Ω, Nafion and ETFE achieved an average power density of 29 mWm-2 compared to 24 mWm-2 for the membrane-less reactors. Over a hypothetical lifetime of 10 years the generated energy (1 to 2.5 kWhm-2) would not be sufficient to offset the costs of any membrane and separator tested. PMID:26305330

40 CFR 434.82 - Effluent limitations attainable by the application of the best practicable control technology...

Code of Federal Regulations, 2013 CFR

2013-07-01

... application of the best practicable control technology currently available (BPT). 434.82 Section 434.82... practicable control technology currently available (BPT). Except as provided in 40 CFR 125.30 through 125.32, the following effluent limitations apply to mine drainage from applicable areas of western coal mining...

40 CFR 434.82 - Effluent limitations attainable by the application of the best practicable control technology...

Code of Federal Regulations, 2010 CFR

2010-07-01

... application of the best practicable control technology currently available (BPT). 434.82 Section 434.82... control technology currently available (BPT). Except as provided in 40 CFR 125.30 through 125.32, the following effluent limitations apply to mine drainage from applicable areas of western coal mining...

40 CFR 434.82 - Effluent limitations attainable by the application of the best practicable control technology...

Code of Federal Regulations, 2014 CFR

2014-07-01

... application of the best practicable control technology currently available (BPT). 434.82 Section 434.82... practicable control technology currently available (BPT). Except as provided in 40 CFR 125.30 through 125.32, the following effluent limitations apply to mine drainage from applicable areas of western coal mining...

40 CFR 434.82 - Effluent limitations attainable by the application of the best practicable control technology...

Code of Federal Regulations, 2012 CFR

2012-07-01

... application of the best practicable control technology currently available (BPT). 434.82 Section 434.82... practicable control technology currently available (BPT). Except as provided in 40 CFR 125.30 through 125.32, the following effluent limitations apply to mine drainage from applicable areas of western coal mining...

40 CFR 434.82 - Effluent limitations attainable by the application of the best practicable control technology...

Code of Federal Regulations, 2011 CFR

2011-07-01

... application of the best practicable control technology currently available (BPT). 434.82 Section 434.82... control technology currently available (BPT). Except as provided in 40 CFR 125.30 through 125.32, the following effluent limitations apply to mine drainage from applicable areas of western coal mining...

DETERMINATION OF AMMONIA MASS EMISSION FLUX FROM HOG WASTE EFFLUENT SPRAYING OPERATION USING OPEN PATH TUNABLE DIODE LASER SPECTROSCOPY WITH VERTICAL RADIAL PLUME MAPPING ANALYSIS

EPA Science Inventory

Emission of ammonia from concentrated animal feeding operations represents an increasingly important environmental issue. Determination of total ammonia mass emission flux from extended area sources such as waste lagoons and waste effluent spraying operations can be evaluated usi...

40 CFR 434.72 - Effluent limitations attainable by the application of the best practicable control technology...

Code of Federal Regulations, 2010 CFR

2010-07-01

... currently available (BPT). (a) The operator must submit a site-specific Pollution Abatement Plan to the permitting authority for the pollution abatement area. The plan must be approved by the permitting authority and incorporated into the permit as an effluent limitation. The Pollution Abatement Plan must identify...

40 CFR 440.43 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2010 CFR

2010-07-01

... in the preamble published on December 3, 1982. (2) In the event that the annual precipitation falling on the treatment facility and the drainage area contributing surface runoff to the treatment facility exceeds the annual evaporation, a volume of water equal to the difference between annual precipitation...

40 CFR 440.13 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2010 CFR

2010-07-01

.... (2) In the event that the annual precipitation falling on the treatment facility and the drainage area contributing surface runoff to the treatment facility exceeds the annual evaporation, a volume of water equal to the difference between annual precipitation falling on the treatment facility and the...

40 CFR 440.12 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2010 CFR

2010-07-01

.... (2) In the event that the annual precipitation falling on the treatment facility and the drainage area contributing surface runoff to the treatment facility exceeds the annual evaporation, a volume of water equivalent to the difference between annual precipitation falling on the treatment facility and...

Glass fiber dissolution in simulated lung fluid and measures needed to improve consistency and correspondence to in vivo dissolution.

PubMed Central

Mattson, S M

1994-01-01

The dissolution of a range of glass fibers including commercial glass and mineral wools has been studied using a modification of Gamble's solution in a flow system at pH 7.4 and 37 degrees C. Dissolution has been followed by weight loss, effluent analysis, and morphology change of fibers and bulk glass. Flow per glass surface area can strongly affect both dissolution rate and morphology due to the effect of the dissolution process on the fluid. Effluent pH is shown to be a guide for choice of optimum flow/area conditions. These conditions provide measurable concentrations of dissolved glass in the effluent while maintaining their concentrations below the point at which they significantly affect the dissolution process. SiO2 and Al2O3 vary widely in the extent to which they are involved in the leaching process, which removes alkalis, alkaline earths, and B2O3. This makes analysis of a single component in the effluent unsuitable as a means of comparing the dissolution rates of a wide range of compositions. PMID:7882963

Dissection of the Caffeate Respiratory Chain in the Acetogen Acetobacterium woodii: Identification of an Rnf-Type NADH Dehydrogenase as a Potential Coupling Site▿

PubMed Central

Imkamp, Frank; Biegel, Eva; Jayamani, Elamparithi; Buckel, Wolfgang; Müller, Volker

2007-01-01

The anaerobic acetogenic bacterium Acetobacterium woodii couples caffeate reduction with electrons derived from hydrogen to the synthesis of ATP by a chemiosmotic mechanism with sodium ions as coupling ions, a process referred to as caffeate respiration. We addressed the nature of the hitherto unknown enzymatic activities involved in this process and their cellular localization. Cell extract of A. woodii catalyzes H2-dependent caffeate reduction. This reaction is strictly ATP dependent but can be activated also by acetyl coenzyme A (CoA), indicating that there is formation of caffeyl-CoA prior to reduction. Two-dimensional gel electrophoresis revealed proteins present only in caffeate-grown cells. Two proteins were identified by electrospray ionization-mass spectrometry/mass spectrometry, and the encoding genes were cloned. These proteins are very similar to subunits α (EtfA) and β (EtfB) of electron transfer flavoproteins present in various anaerobic bacteria. Western blot analysis demonstrated that they are induced by caffeate and localized in the cytoplasm. Etf proteins are known electron carriers that shuttle electrons from NADH to different acceptors. Indeed, NADH was used as an electron donor for cytosolic caffeate reduction. Since the hydrogenase was soluble and used ferredoxin as an electron acceptor, the missing link was a ferredoxin:NAD+ oxidoreductase. This activity could be determined and, interestingly, was membrane bound. A search for genes that could encode this activity revealed DNA fragments encoding subunits C and D of a membrane-bound Rnf-type NADH dehydrogenase that is a potential Na+ pump. These data suggest the following electron transport chain: H2 → ferredoxin → NAD+ → Etf → caffeyl-CoA reductase. They also imply that the sodium motive step in the chain is the ferredoxin-dependent NAD+ reduction catalyzed by Rnf. PMID:17873051

Sites of superoxide and hydrogen peroxide production during fatty acid oxidation in rat skeletal muscle mitochondria.

PubMed

Perevoshchikova, Irina V; Quinlan, Casey L; Orr, Adam L; Gerencser, Akos A; Brand, Martin D

2013-08-01

H2O2 production by skeletal muscle mitochondria oxidizing palmitoylcarnitine was examined under two conditions: the absence of respiratory chain inhibitors and the presence of myxothiazol to inhibit complex III. Without inhibitors, respiration and H2O2 production were low unless carnitine or malate was added to limit acetyl-CoA accumulation. With palmitoylcarnitine alone, H2O2 production was dominated by complex II (44% from site IIF in the forward reaction); the remainder was mostly from complex I (34%, superoxide from site IF). With added carnitine, H2O2 production was about equally shared between complexes I, II, and III. With added malate, it was 75% from complex III (superoxide from site IIIQo) and 25% from site IF. Thus complex II (site IIF in the forward reaction) is a major source of H2O2 production during oxidation of palmitoylcarnitine ± carnitine. Under the second condition (myxothiazol present to keep ubiquinone reduced), the rates of H2O2 production were highest in the presence of palmitoylcarnitine ± carnitine and were dominated by complex II (site IIF in the reverse reaction). About half the rest was from site IF, but a significant portion, ∼40pmol H2O2·min(-1)·mg protein(-1), was not from complex I, II, or III and was attributed to the proteins of β-oxidation (electron-transferring flavoprotein (ETF) and ETF-ubiquinone oxidoreductase). The maximum rate from the ETF system was ∼200pmol H2O2·min(-1)·mg protein(-1) under conditions of compromised antioxidant defense and reduced ubiquinone pool. Thus complex II and the ETF system both contribute to H2O2 productionduring fatty acid oxidation under appropriate conditions. Copyright © 2013 Elsevier Inc. All rights reserved.

Transcription factors ETF, E2F, and SP-1 are involved in cytokine-independent proliferation of murine hepatocytes.

PubMed

Zellmer, Sebastian; Schmidt-Heck, Wolfgang; Godoy, Patricio; Weng, Honglei; Meyer, Christoph; Lehmann, Thomas; Sparna, Titus; Schormann, Wiebke; Hammad, Seddik; Kreutz, Clemens; Timmer, Jens; von Weizsäcker, Fritz; Thürmann, Petra A; Merfort, Irmgard; Guthke, Reinhard; Dooley, Steven; Hengstler, Jan G; Gebhardt, Rolf

2010-12-01

The cellular basis of liver regeneration has been intensely investigated for many years. However, the mechanisms initiating hepatocyte "plasticity" and priming for proliferation are not yet fully clear. We investigated alterations in gene expression patterns during the first 72 hours of C57BL/6N mouse hepatocyte culture on collagen monolayers (CM), which display a high basal frequency of proliferation in the absence of cytokines. Although many metabolic genes were down-regulated, genes related to mitogen-activated protein kinase (MAPK) signaling and cell cycle were up-regulated. The latter genes showed an overrepresentation of transcription factor binding sites (TFBS) for ETF (TEA domain family member 2), E2F1 (E2F transcription factor 1), and SP-1 (Sp1 transcription factor) (P < 0.001), all depending on MAPK signaling. Time-dependent increase of ERK1/2 phosphorylation occurred during the first 48 hours (and beyond) in the absence of cytokines, accompanied by an enhanced bromodeoxyuridine labeling index of 20%. The MEK inhibitor PD98059 blunted these effects indicating MAPK signaling as major trigger for this cytokine-independent proliferative response. In line with these in vitro findings, liver tissue of mice challenged with CCl(4) displayed hepatocytes with intense p-ERK1/2 staining and nuclear SP-1 and E2F1 expression. Furthermore, differentially expressed genes in mice after partial hepatectomy contained overrepresented TFBS for ETF, E2F1, and SP-1 and displayed increased expression of E2F1. Cultivation of murine hepatocytes on CM primes cells for proliferation through cytokine-independent activation of MAPK signaling. The transcription factors ETF, E2F1, and SP-1 seem to play a pronounced role in mediating proliferation-dependent differential gene expression. Similar events, but on a shorter time-scale, occur very early after liver damage in vivo. Copyright © 2010 American Association for the Study of Liver Diseases.

Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase

PubMed Central

Lin, Mingqun; Liu, Hongyan; Xiong, Qingming; Niu, Hua; Cheng, Zhihui; Yamamoto, Akitsugu; Rikihisa, Yasuko

2016-01-01

Ehrlichia chaffeensis is an obligatory intracellular bacterium that causes a potentially fatal emerging zoonosis, human monocytic ehrlichiosis. E. chaffeensis has a limited capacity for biosynthesis and metabolism and thus depends mostly on host-synthesized nutrients for growth. Although the host cell cytoplasm is rich with these nutrients, as E. chaffeensis is confined within the early endosome-like membrane-bound compartment, only host nutrients that enter the compartment can be used by this bacterium. How this occurs is unknown. We found that ehrlichial replication depended on autophagy induction involving class III phosphatidylinositol 3-kinase (PtdIns3K) activity, BECN1 (Beclin 1), and ATG5 (autophagy-related 5). Ehrlichia acquired host cell preincorporated amino acids in a class III PtdIns3K-dependent manner and ehrlichial growth was enhanced by treatment with rapamycin, an autophagy inducer. Moreover, ATG5 and RAB5A/B/C were routed to ehrlichial inclusions. RAB5A/B/C siRNA knockdown, or overexpression of a RAB5-specific GTPase-activating protein or dominant-negative RAB5A inhibited ehrlichial infection, indicating the critical role of GTP-bound RAB5 during infection. Both native and ectopically expressed ehrlichial type IV secretion effector protein, Etf-1, bound RAB5 and the autophagy-initiating class III PtdIns3K complex, PIK3C3/VPS34, and BECN1, and homed to ehrlichial inclusions. Ectopically expressed Etf-1 activated class III PtdIns3K as in E. chaffeensis infection and induced autophagosome formation, cleared an aggregation-prone mutant huntingtin protein in a class III PtdIns3K-dependent manner, and enhanced ehrlichial proliferation. These data support the notion that E. chaffeensis secretes Etf-1 to induce autophagy to repurpose the host cytoplasm and capture nutrients for its growth through RAB5 and class III PtdIns3K, while avoiding autolysosomal killing. PMID:27541856

Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase.

PubMed

Lin, Mingqun; Liu, Hongyan; Xiong, Qingming; Niu, Hua; Cheng, Zhihui; Yamamoto, Akitsugu; Rikihisa, Yasuko

2016-11-01

Ehrlichia chaffeensis is an obligatory intracellular bacterium that causes a potentially fatal emerging zoonosis, human monocytic ehrlichiosis. E. chaffeensis has a limited capacity for biosynthesis and metabolism and thus depends mostly on host-synthesized nutrients for growth. Although the host cell cytoplasm is rich with these nutrients, as E. chaffeensis is confined within the early endosome-like membrane-bound compartment, only host nutrients that enter the compartment can be used by this bacterium. How this occurs is unknown. We found that ehrlichial replication depended on autophagy induction involving class III phosphatidylinositol 3-kinase (PtdIns3K) activity, BECN1 (Beclin 1), and ATG5 (autophagy-related 5). Ehrlichia acquired host cell preincorporated amino acids in a class III PtdIns3K-dependent manner and ehrlichial growth was enhanced by treatment with rapamycin, an autophagy inducer. Moreover, ATG5 and RAB5A/B/C were routed to ehrlichial inclusions. RAB5A/B/C siRNA knockdown, or overexpression of a RAB5-specific GTPase-activating protein or dominant-negative RAB5A inhibited ehrlichial infection, indicating the critical role of GTP-bound RAB5 during infection. Both native and ectopically expressed ehrlichial type IV secretion effector protein, Etf-1, bound RAB5 and the autophagy-initiating class III PtdIns3K complex, PIK3C3/VPS34, and BECN1, and homed to ehrlichial inclusions. Ectopically expressed Etf-1 activated class III PtdIns3K as in E. chaffeensis infection and induced autophagosome formation, cleared an aggregation-prone mutant huntingtin protein in a class III PtdIns3K-dependent manner, and enhanced ehrlichial proliferation. These data support the notion that E. chaffeensis secretes Etf-1 to induce autophagy to repurpose the host cytoplasm and capture nutrients for its growth through RAB5 and class III PtdIns3K, while avoiding autolysosomal killing.

Effluent migration from septic tank systems in two different lithologies, Broward County, Florida

USGS Publications Warehouse

Waller, B.G.; Howie, Barbara; Causaras, C.R.

1987-01-01

Two septic tank test sites, one in sand and one in limestone, in Broward County, Florida, were analyzed for effluent migration. Groundwater from shallow wells, both in background areas and hydraulically down-gradient of the septic tank system, was sampled during a 16-month period from April 1983 through August 1984. Water quality indicators were used to determine the effluent affected zone near the septic tank systems. Specific conductance levels and concentrations of chloride, sulfate, ammonium, and nitrate indicated effluent movement primarily in a vertical direction with abrupt dilution as it moved down-gradient. Effluent was detected in the sand to a depth more than 20 ft below the septic tank outlet, but was diluted to near background conditions 50 ft down-gradient from the tank. Effluent in the limestone was detected in all three observation wells to depths exceeding 25 ft below the septic tank outlet and was diluted, but still detectable, 40 ft down-gradient. The primary controls on effluent movement from septic tank systems in Broward County are the lithology and layering of the geologic materials, hydraulic gradients, and the volume and type of use the system receives. (Author 's abstract)

Impacts and Policy Implications of Metals Effluent Discharge into Rivers within Industrial Zones: A Sub-Saharan Perspective from Ethiopia

NASA Astrophysics Data System (ADS)

Zinabu, E.; Kelderman, P.; van der Kwast, J.; Irvine, K.

2018-04-01

Kombolcha, a city in Ethiopia, exemplifies the challenges and problems of the sub-Saharan countries where industrialization is growing fast but monitoring resources are poor and information on pollution unknown. This study monitored metals Cr, Cu, Zn, and Pb concentrations in five factories' effluents, and in the effluent mixing zones of two rivers receiving discharges during the rainy seasons of 2013 and 2014. The results indicate that median concentrations of Cr in the tannery effluents and Zn in the steel processing effluents were as high as 26,600 and 155,750 µg/L, respectively, much exceeding both the USEPA and Ethiopian emission guidelines. Cu concentrations were low in all effluents. Pb concentrations were high in the tannery effluent, but did not exceed emission guidelines. As expected, no metal emission guidelines were exceeded for the brewery, textile and meat processing effluents. Median Cr and Zn concentrations in the Leyole river in the effluent mixing zones downstream of the tannery and steel processing plant increased by factors of 52 (2660 compared with 51 µg Cr/L) and 5 (520 compared with 110 µg Zn/L), respectively, compared with stations further upstream. This poses substantial ecological risks downstream. Comparison with emission guidelines indicates poor environmental management by industries and regulating institutions. Despite appropriate legislation, no clear measures have yet been taken to control industrial discharges, with apparent mismatch between environmental enforcement and investment policies. Effluent management, treatment technologies and operational capacity of environmental institutions were identified as key improvement areas to adopt progressive sustainable development.

Dielectrophoretic particle-particle interaction under AC electrohydrodynamic flow conditions.

PubMed

Lee, Doh-Hyoung; Yu, Chengjie; Papazoglou, Elisabeth; Farouk, Bakhtier; Noh, Hongseok M

2011-09-01

We used the Maxwell stress tensor method to understand dielectrophoretic particle-particle interactions and applied the results to the interpretation of particle behaviors under alternating current (AC) electrohydrodynamic conditions such as AC electroosmosis (ACEO) and electrothermal flow (ETF). Distinct particle behaviors were observed under ACEO and ETF. Diverse particle-particle interactions observed in experiments such as particle clustering, particles keeping a certain distance from each other, chain and disc formation and their rotation, are explained based on the numerical simulation data. The improved understanding of particle behaviors in AC electrohydrodynamic flows presented here will enable researchers to design better particle manipulation strategies for lab-on-a-chip applications. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Magnetohydrodynamics (MHD) Engineering Test Facility (ETF) 200 MWe power plant. Conceptual Design Engineering Report (CDER). Volume 4: Supplementary engineering data

NASA Astrophysics Data System (ADS)

1981-09-01

The reference conceptual design of the Magnetohydrodynamic Engineering Test Facility (ETF), a prototype 200 MWe coal-fired electric generating plant designed to demonstrate the commercial feasibility of open cycle MHD is summarized. Main elements of the design are identified and explained, and the rationale behind them is reviewed. Major systems and plant facilities are listed and discussed. Construction cost and schedule estimates, and identification of engineering issues that should be reexamined are also given. The latest (1980-1981) information from the MHD technology program are integrated with the elements of a conventional steam power electric generating plant. Supplementary Engineering Data (Issues, Background, Performance Assurance Plan, Design Details, System Design Descriptions and Related Drawings) is presented.

Magnetohydrodynamics (MHD) Engineering Test Facility (ETF) 200 MWe power plant. Conceptual Design Engineering Report (CDER). Volume 4: Supplementary engineering data

NASA Technical Reports Server (NTRS)

1981-01-01

The reference conceptual design of the Magnetohydrodynamic Engineering Test Facility (ETF), a prototype 200 MWe coal-fired electric generating plant designed to demonstrate the commercial feasibility of open cycle MHD is summarized. Main elements of the design are identified and explained, and the rationale behind them is reviewed. Major systems and plant facilities are listed and discussed. Construction cost and schedule estimates, and identification of engineering issues that should be reexamined are also given. The latest (1980-1981) information from the MHD technology program are integrated with the elements of a conventional steam power electric generating plant. Supplementary Engineering Data (Issues, Background, Performance Assurance Plan, Design Details, System Design Descriptions and Related Drawings) is presented.

Unified analytic representation of physical sputtering yield

NASA Astrophysics Data System (ADS)

Janev, R. K.; Ralchenko, Yu. V.; Kenmotsu, T.; Hosaka, K.

2001-03-01

Generalized energy parameter η= η( ɛ, δ) and normalized sputtering yield Ỹ(η) , where ɛ= E/ ETF and δ= Eth/ ETF, are introduced to achieve a unified representation of all available experimental and sputtering data at normal ion incidence. The sputtering data in the new Ỹ(η) representation retain their original uncertainties. The Ỹ(η) data can be fitted to a simple three-parameter analytic expression with an rms deviation of 32%, well within the uncertainties of original data. Both η and Ỹ(η) have correct physical behavior in the threshold and high-energy regions. The available theoretical data produced by the TRIM.SP code can also be represented by the same single analytic function Ỹ(η) with a similar accuracy.

Trace Analysis of Heavy Metals in Ground Waters of Vijayawada Industrial Area

ERIC Educational Resources Information Center

Tadiboyina, Ravisankar; Ptsrk, Prasada Rao

2016-01-01

In recent years, the new environmental problem are arising due to industrial hazard wastage, global climate change, ground water contamination and etc., gives an attention to protect environment.one of the major source of contamination of ground water is improper discharge of industrial effluents these effluents contains so many heavy metals which…

Duckweed based wastewater stabilization ponds for wastewater treatment (a low cost technology for small urban areas in Zimbabwe)

NASA Astrophysics Data System (ADS)

Dalu, J. M.; Ndamba, J.

A three-year investigation into the potential use of duckweed based wastewater stabilizations ponds for wastewater treatment was carried out at two small urban areas in Zimbabwe. The study hoped to contribute towards improved environmental management through improving the quality of effluent being discharged into natural waterways. This was to be achieved through the development and facilitation of the use of duckweed based wastewater stabilizations ponds. The study was carried out at Nemanwa and Gutu Growth Points both with a total population of 23 000. The two centers, like more than 70% of Zimbabwe’s small urban areas, relied on algae based ponds for domestic wastewater treatment. The final effluent is used to irrigate gum plantations before finding its way into the nearest streams. Baseline wastewater quality information was collected on a monthly basis for three months after which duckweed ( Lemna minor) was introduced into the maturation ponds to at least 50% pond surface cover. The influent and effluent was then monitored on a monthly basis for chemical, physical and bacteriological parameters as stipulated in the Zimbabwe Water (Waste and Effluent Disposal) regulations of 2000. After five months, the range of parameters tested for was narrowed to include only those that sometimes surpassed the limits. These included: phosphates, nitrates, pH, biological oxygen demand, iron, conductivity, chemical oxygen demand, turbidity, total dissolved solids and total suspended solids. Significant reductions to within permissible limits were obtained for most of the above-mentioned parameters except for phosphates, chemical and biological oxygen demand and turbidity. However, in these cases, more than 60% reductions were observed when the influent and effluent levels were compared. It is our belief that duckweed based waste stabilization ponds can now be used successfully for the treatment of domestic wastewater in small urban areas of Zimbabwe.

ETF magnet design alternatives for the national MHD program

NASA Astrophysics Data System (ADS)

Marston, P. G.; Thome, R. J.; Dawson, A. M.; Bobrov, E. S.; Hatch, A. M.

1981-01-01

Five superconducting magnet designs are evaluated for a 200 MWe test facility requiring a magnet with an on-axis field of 6 T, an inlet bore area of 4 sq m, storing 6 x 10 to the 9th J. The designs include a straightforward rectangular saddle coil set, a 'Cask' configuration based on staves and corner blocks as the main support structure, and an internally cooled, cabled superconductor to minimize the substructure and eliminate the helium vessel. Also, a modular design using six coils with individual helium vessels and an integrated structure produces a simplest configuration which utilizes a natural rectangular interface for packaging the MHD channel and its connections, and results in a lower capital cost.

DC-7B Aircraft Spray System for Large-Area Insect Control

DTIC Science & Technology

1975-11-01

the ground. The two inert aerosol samnplers used were a 2.5-inch-diameter can wrapped with Printflex-card stock and a 2-mm-diameter pipe cleaner. The...mph (i to 6 m/sec). (2) One inert aerosol sampler (can wrapped with Printflex- card stock ) was positioned 20, 40, 60, and 80 meters above terrain on...4.040x105 2.174x105 9.195x104 2.950x105 1. 035x10 5 ETf 0.501 0.927 1.00 0.501 0.501 L (m) 1.408x104 7.376x103 7.488x103 1.028x104 8.662x103 H (i) 15

40 CFR 421.303 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

...-gas wet air pollution control. BAT Limitations for the Primary and Secondary Titanium Subcategory... pounds) of TiCl4 produced Chromium (total) 0.346 0.140 Lead 0.262 0.122 Nickel 0.515 0.346 Titanium 0.496 0.215 (b) Chlorination area-vent wet air pollution control. BAT Limitations for the Primary and...

40 CFR 421.303 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

...-gas wet air pollution control. BAT Limitations for the Primary and Secondary Titanium Subcategory... pounds) of TiCl4 produced Chromium (total) 0.346 0.140 Lead 0.262 0.122 Nickel 0.515 0.346 Titanium 0.496 0.215 (b) Chlorination area-vent wet air pollution control. BAT Limitations for the Primary and...

Novel ETF dehydrogenase mutations in a patient with mild glutaric aciduria type II and complex II-III deficiency in liver and muscle

PubMed Central

Wolfe, Lynne A.; He, Miao; Vockley, Jerry; Payne, Nicole; Rhead, William; Hoppel, Charles; Spector, Elaine; Gernert, Kim; Gibson, K. Michael

2014-01-01

We describe a 22-year-old male who developed severe hypoglycemia and lethargy during an acute illness at 4 months of age and subsequently grew and developed normally. At age 4 years he developed recurrent vomiting with mild hyperammonemia and dehydration requiring frequent hospitalizations. Glutaric aciduria Type II was suspected based upon biochemical findings and managed with cornstarch, carnitine and riboflavin supplements. He did not experience metabolic crises between ages 4-12 years. He experienced recurrent vomiting, mild hyperammonemia, and generalized weakness associated with acute illnesses and growth spurts. At age 18 years, he developed exercise intolerance and proximal muscle weakness leading to the identification of multiple acyl-CoAdehydrogenase and complex II/III deficiencies in both skeletal muscle and liver. Subsequent molecular characterization of the ETFDH gene revealed novel heterozygous mutations, p.G274X:c.820 G>T (exon 7) and p.P534L: c.1601 C>T (exon 12), the latter within the iron sulfur-cluster and predicted to affect ubiquinone reductase activity of ETFDH and the docking of ETF to ETFDH. Our case supports the concept of a structural interaction between ETFDH and other enzyme partners, and suggests that the conformational change upon ETF binding to ETFDH may play a key role in linking ETFDH to II/III super-complex formation. PMID:21088898

Novel ETF dehydrogenase mutations in a patient with mild glutaric aciduria type II and complex II-III deficiency in liver and muscle.

PubMed

Wolfe, Lynne A; He, Miao; Vockley, Jerry; Payne, Nicole; Rhead, William; Hoppel, Charles; Spector, Elaine; Gernert, Kim; Gibson, K Michael

2010-12-01

We describe a 22-year-old male who developed severe hypoglycemia and lethargy during an acute illness at 4 months of age and subsequently grew and developed normally. At age 4 years he developed recurrent vomiting with mild hyperammonemia and dehydration requiring frequent hospitalizations. Glutaric aciduria Type II was suspected based upon biochemical findings and managed with cornstarch, carnitine and riboflavin supplements. He did not experience metabolic crises between ages 4-12 years. He experienced recurrent vomiting, mild hyperammonemia, and generalized weakness associated with acute illnesses and growth spurts. At age 18 years, he developed exercise intolerance and proximal muscle weakness leading to the identification of multiple acyl-CoA dehydrogenase and complex II/III deficiencies in both skeletal muscle and liver. Subsequent molecular characterization of the ETFDH gene revealed novel heterozygous mutations, p.G274X:c.820 G > T (exon 7) and p.P534L: c.1601 C > T (exon 12), the latter within the iron sulfur-cluster and predicted to affect ubiquinone reductase activity of ETFDH and the docking of ETF to ETFDH. Our case supports the concept of a structural interaction between ETFDH and other enzyme partners, and suggests that the conformational change upon ETF binding to ETFDH may play a key role in linking ETFDH to II/III super-complex formation.

The synthesis of branched TCP chromophores and the research on their electro-optical properties

NASA Astrophysics Data System (ADS)

Bo, Shuhui; Chen, Zhuo; Gao, Wu; Zhen, Zhen; Liu, Xinhou

2012-10-01

In order to minimize the intermolecular electrostatic interactions and effectively translate high value of chromophore into macroscopic electro-optical (EO) coeffcient (r33), the shape-modification of aniline-pyrroline (TCP) chromophore by combining three kinds of dendritic groups respectively to the N atom of pyrroline acceptor produced three kinds of dendritic chromophores. Their spherical structures can minimize intermolecular electrostatic interactions, and thus the poling efficience was higher than the chromophores without dendritic groups when chromophores as a guest in the host polymer APC. A large electro-optical (EO) coefficient was achieved as high as 75 pm/V at 1315 nm with 9% chromophores loading in APC film. On the basis of the above TCP chromophores, two kinds of novel molecular glasses based on self-assembly dendritic chromophores are also designed and synthesized as second-order nonlinear optical (NLO) materials, which named ETO and ETF. The NLO chromophore glasses ETO and ETF showed excellent filmforming ability by themselves. Their glass transition temperatures (Tg) were determined at 41° and 39°, respectively. The in-situ second harmonic generation (SHG) measurement revealed the resonant electro-optical (EO) coefficient (d33) values of 38 and 32 pm/V for the poled films of ETO and ETF, respectively. The results indicate molecular glasses provide a new possible way different from the conventional polymer approach to prepare second-order NLO materials.

Detoxification of kraft pulp ECF bleaching effluents by catalytic hydrotreatment.

PubMed

Calvo, L; Gilarranz, M A; Casas, J A; Mohedano, A F; Rodríguez, J J

2007-02-01

Two different effluents from the D(1) and E(1) stages of the ECF bleaching of Eucalyptus globulus kraft pulp were treated by catalytic hydrogenation in a trickle bed reactor using commercial and homemade Pd/AC catalysts. The reactor was fed with the bleaching effluent and a H(2)/N(2) gas stream. The variables studied were space-time (1.4-5g(cat)min/mL), gas to liquid flow ratio (286-1000vol.), gas feed concentration (H(2):N(2), 1:1-1:7.3vol.), temperature (25-100 degrees C) and pressure (1-11bar). Hydrotreatment performance was evaluated in terms of ecotoxicity, adsorbable organic halogen (AOX), chemical oxygen demand (COD), biological oxygen demand (BOD(5)) and colour removal. In all the runs, the ecotoxicity of the effluents decreased as a result of the treatment, achieving reductions that ranged from 70% to 98%. Simultaneously to the reduction of toxicity, the hydrotreatment led to a decrease of the colour of the effluents, being the decrease significantly higher in the case of E(1) effluent. The AOX content was reduced by 85% and 23% for E(1) and D(1) effluents, respectively. In the case of D(1) effluent the removal of ecotoxicity was significantly higher than that of AOX, which indicates that much of the toxicity of the effluent must be associated to non-chlorinated organics. In spite of the important reduction of ecotoxicity, the biodegradability of the effluents only increased slightly. The homemade catalysts, prepared from activated carbons with a high external or non-microporous surface area and mesopore volume and a convenient surface chemistry showed a higher efficiency than the commercial one.

Organogenesis of heart-vascular system derived from mouse 2 cell stage embryos and from early embryonic stem cells in vitro.

PubMed

Ishiwata, Isamu; Tamagawa, Tomoharu; Tokieda, Yuko; Iguchi, Megumi; Sato, Kahei; Ishikawa, Hiroshi

2003-03-01

Regenerative medical treatment with embryonic stem cells (an ES cell) is a goal for organ transplantation. Structures that are tubular in nature (i.e. blood capillaries) were induced from early embryonic stem (EES) cells in vitro using embryotrophic factor (ETFs). In addition, cardiac muscle cells could be identified as well. However, differentiation of EES cells into a complete cardiovascular system was difficult because 3 germ layer primordial organs are directed embryologically in various ways and it is not possible to guide only cardiovascular organs. Thus, we introduced ETFs after the formation of an embryoid body and were successful in cloning cell clusters that beat, thus deriving only cardiovascular organs. The application of this to the treatment of various cardiovascular diseases is promising.

Settling and survival profile of enteric pathogens in the swine effluent for water reuse purpose.

PubMed

Fongaro, G; Kunz, A; Magri, M E; Schissi, C D; Viancelli, A; Philippi, L S; Barardi, C R M

2016-11-01

The present study evaluated the pathogens persistence and settling profile in swine effluent. We determined the enteric pathogens settling characteristics, their survival and inactivation profile in swine effluent (for water reuse purpose) and in sludge (generated after aerobic treatment - during secondary settling process). The study was performed in laboratorial-scale and in full-scale (manure treatment plant). Enteric viruses and enteric bacteria were used as biomarkers. Results showed that these enteric pathogens were significantly reduced from swine effluent during secondary settling process, and enteric viruses removal was correlated with the suspended solids decantation. The design of secondary settlers can be adapted to improve pathogens removal, by diminishing the solids loading rate per area and time, ending in higher hydraulic retention times. Copyright © 2016 Elsevier GmbH. All rights reserved.

Cell Phone ETF Act

THOMAS, 111th Congress

Sen. Klobuchar, Amy [D-MN

2009-12-03

Senate - 12/03/2009 Read twice and referred to the Committee on Commerce, Science, and Transportation. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Environmental effects of dredging. Documentation of the efqual module for ADDAMS: Comparison of predicted effluent water quality with standards. Technical notes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Palermo, M.R.; Schroeder, P.R.

This technical note describes a technique for comparison of the predicted quality of effluent discharged from confined dredged material disposal areas with applicable water quality standards. This note also serves as documentation of a computer program called EFQUAL written for that purpose as part of the Automated Dredging and Disposal Alternatives Management System (ADDAMS).

Effect of sugar factory effluent on some physico-chemical properties of soils--a case study.

PubMed

Roy, Ratna P; Prasad, Jagdish; Joshi, A P

2007-10-01

The effect of irrigation by sugar factory effluent (spentwash) and the well water from adjoining area has been studied in Wardha district, Maharashtra. The effluent had high TDS (422-608 mgL(-1)), COD (1152-17680 mgL(-1)) and BOD(380-650 mgL(-1)) than well water (TDS 240 mgL(-1), COD 3.8 mgL(-1) and BOD 1.2 mgL(-1)). There found some nutrients, viz. N, P, K, Zn, Cu, Fe, Mn in surface layer of soil in different seasons. Heavy metals (Cd, Co, Cr, Ni, Pb) were found to be within the permissible limits.

Groundwater impact assessment report for the 216-S-26 Crib, 200 West Area

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lindberg, J.W.; Evelo, S.D.; Alexander, D.J.

1993-11-01

This report assesses the impact of wastewater discharged to the 216-S-26 Crib on groundwater quality. The 216-S-26 Crib, located in the southern 200 West Area, has been in use since 1984 to dispose of liquid effluents from the 222-S Laboratory Complex. The 222-S Laboratory Complex effluent stream includes wastewater from four sources: the 222-S Laboratory, the 219-S Waste Storage Facility, the 222-SA Chemical Standards Laboratory, and the 291-S Exhaust Fan Control House and Stack. Based on assessment of groundwater chemistry and flow data, contaminant transport predictions, and groundwater chemistry data, the 216-S-26 Crib has minimal influence on groundwater contamination inmore » the southern 200 West Area.« less

State waste discharge permit application: 200 Area Treated Effluent Disposal Facility (Project W-049H)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1994-08-01

As part of the original Hanford Federal Facility Agreement and Concent Order negotiations, US DOE, US EPA and the Washington State Department of Ecology agreed that liquid effluent discharges to the ground to the Hanford Site are subject to permitting in the State Waste Discharge Permit Program (SWDP). This document constitutes the SWDP Application for the 200 Area TEDF stream which includes the following streams discharged into the area: Plutonium Finishing Plant waste water; 222-S laboratory Complex waste water; T Plant waste water; 284-W Power Plant waste water; PUREX chemical Sewer; B Plant chemical sewer, process condensate, steam condensate; 242-A-81more » Water Services waste water.« less

a Time-Dependent Many-Electron Approach to Atomic and Molecular Interactions

NASA Astrophysics Data System (ADS)

Runge, Keith

A new methodology is developed for the description of electronic rearrangement in atomic and molecular collisions. Using the eikonal representation of the total wavefunction, time -dependent equations are derived for the electronic densities within the time-dependent Hartree-Fock approximation. An averaged effective potential which ensures time reversal invariance is used to describe the effect of the fast electronic transitions on the slower nuclear motions. Electron translation factors (ETF) are introduced to eliminate spurious asymptotic couplings, and a local ETF is incorporated into a basis of traveling atomic orbitals. A reference density is used to describe local electronic relaxation and to account for the time propagation of fast and slow motions, and is shown to lead to an efficient integration scheme. Expressions for time-dependent electronic populations and polarization parameters are given. Electronic integrals over Gaussians including ETFs are derived to extend electronic state calculations to dynamical phenomena. Results of the method are in good agreement with experimental data for charge transfer integral cross sections over a projectile energy range of three orders of magnitude in the proton-Hydrogen atom system. The more demanding calculations of integral alignment, state-to-state integral cross sections, and differential cross sections are found to agree well with experimental data provided care is taken to include ETFs in the calculation of electronic integrals and to choose the appropriate effective potential. The method is found to be in good agreement with experimental data for the calculation of charge transfer integral cross sections and state-to-state integral cross sections in the one-electron heteronuclear Helium(2+)-Hydrogen atom system and in the two-electron system, Hydrogen atom-Hydrogen atom. Time-dependent electronic populations are seen to oscillate rapidly in the midst of collision event. In particular, multiple exchanges of the electron are seen to occur in the proton-Hydrogen atom system at low collision energies. The concepts and results derived from the approach provide new insight into the dynamics of nuclear screening and electronic rearrangement in atomic collisions.

The influence of outdoor thermal environment on young Japanese females.

PubMed

Kurazumi, Yoshihito; Ishii, Jin; Kondo, Emi; Fukagawa, Kenta; Bolashikov, Zhecho Dimitrov; Sakoi, Tomonori; Tsuchikawa, Tadahiro; Matsubara, Naoki; Horikoshi, Tetsumi

2014-07-01

The influence of short wave solar radiation appears to be strong outdoors in summer, and the influence of airflow appears to be strong outdoors in winter. The purpose of this paper was to clarify the influence of the outdoor environment on young Japanese females. This research shows the relationship between the physiological and psychological responses of humans and the enhanced conduction-corrected modified effective temperature (ETFe). Subjective experiments were conducted in an outdoor environment. Subjects were exposed to the thermal environment in a standing posture. Air temperature, humidity, air velocity, short wave solar radiation, long wave radiation, ground surface temperature, sky factor, and the green solid angle were measured. The temperatures of skin exposed to the atmosphere and in contact with the ground were measured. Thermal sensation and thermal comfort were measured by means of rating the whole-body thermal sensation (cold-hot) and the whole body thermal comfort (comfortable-uncomfortable) on a linear scale. Linear rating scales are given for the hot (100) and cold (0), and comfortable (100) and uncomfortable (0) directions only. Arbitrary values of 0 and 100 were assigned to each endpoint, the reported values read in, and the entire length converted into a numerical value with an arbitrary scale of 100 to give a linear rating scale. The ETFe considered to report a neither hot nor cold, thermally neutral sensation of 50 was 35.9 °C, with 32.3 °C and 42.9 °C, respectively, corresponding to the low and high temperature ends of the ETFe considered to report a neither comfortable nor uncomfortable comfort value of 50. The mean skin temperature considered to report a neither hot nor cold, thermally neutral sensation of 50 was 33.3 °C, with 31.0 °C and 34.3 °C, respectively, corresponding to the low and high temperature ends of the mean skin temperature considered to report a neither comfortable nor uncomfortable comfort value of 50. The acceptability raised the mean skin temperature even for thermal environment conditions in which ETFe was high.

The influence of outdoor thermal environment on young Japanese females

NASA Astrophysics Data System (ADS)

Kurazumi, Yoshihito; Ishii, Jin; Kondo, Emi; Fukagawa, Kenta; Bolashikov, Zhecho Dimitrov; Sakoi, Tomonori; Tsuchikawa, Tadahiro; Matsubara, Naoki; Horikoshi, Tetsumi

2014-07-01

The influence of short wave solar radiation appears to be strong outdoors in summer, and the influence of airflow appears to be strong outdoors in winter. The purpose of this paper was to clarify the influence of the outdoor environment on young Japanese females. This research shows the relationship between the physiological and psychological responses of humans and the enhanced conduction-corrected modified effective temperature (ETFe). Subjective experiments were conducted in an outdoor environment. Subjects were exposed to the thermal environment in a standing posture. Air temperature, humidity, air velocity, short wave solar radiation, long wave radiation, ground surface temperature, sky factor, and the green solid angle were measured. The temperatures of skin exposed to the atmosphere and in contact with the ground were measured. Thermal sensation and thermal comfort were measured by means of rating the whole-body thermal sensation (cold-hot) and the whole body thermal comfort (comfortable-uncomfortable) on a linear scale. Linear rating scales are given for the hot (100) and cold (0), and comfortable (100) and uncomfortable (0) directions only. Arbitrary values of 0 and 100 were assigned to each endpoint, the reported values read in, and the entire length converted into a numerical value with an arbitrary scale of 100 to give a linear rating scale. The ETFe considered to report a neither hot nor cold, thermally neutral sensation of 50 was 35.9 °C, with 32.3 °C and 42.9 °C, respectively, corresponding to the low and high temperature ends of the ETFe considered to report a neither comfortable nor uncomfortable comfort value of 50. The mean skin temperature considered to report a neither hot nor cold, thermally neutral sensation of 50 was 33.3 °C, with 31.0 °C and 34.3 °C, respectively, corresponding to the low and high temperature ends of the mean skin temperature considered to report a neither comfortable nor uncomfortable comfort value of 50. The acceptability raised the mean skin temperature even for thermal environment conditions in which ETFe was high.

Soil plutonium and cesium in stream channels and banks of Los Alamos liquid effluent-receiving areas.

PubMed

Nyhan, J W; White, G C; Trujillo, G

1982-10-01

Stream channel sediments and adjacent bank soils found in three intermittent streams used for treated liquid effluent disposal at Los Alamos, New Mexico were sampled to determine the distribution of 238Pu, 239,240Pu and 137Cs. Radionuclide concentrations and inventories were determined as functions of distance downstream from the waste outfall and from the center of the stream channel, soil sampling depth, stream channel-bank physiography, and the waste use history of each disposal area. Radionuclide concentrations in channel sediments were inversely related to distances up to 10 km downstream from the outfalls. For sites receiving appreciable waste effluent additions, contaminant concentrations in bank soils decreased with perpendicular distances greater than 0.38 m from the stream channel, and with stream bank sampling depths greater than 20-40 cm. Concentrations and total inventories of radionuclides in stream bank soils generally decreased as stream bank height increased. Inventory estimates of radionuclides in channel sediments exhibited coefficients of variation that ranged 0.41-2.6, reflecting the large variation in radionuclide concentrations at each site. Several interesting temporal relationships of these radionuclides in intermittent streams were gleaned from the varying waste use histories of the three effluent-receiving areas. Eleven yr after liquid wastes were added to one canyon, the major radionuclide inventories were found in the stream bank soils, unlike most of the other currently-used receiving areas. A period of time greater than 6 yr seems to be required before the plutonium in liquid wastes currently added to the canyon is approximately equilibrated with the plutonium in the bank soils. These observations are discussed relative to waste management practices in these southwestern intermittent streams.

Forms of phosphorus transfer in runoff under no-tillage in a soil treated with successive swine effluents applications.

PubMed

Lourenzi, Cledimar Rogério; Ceretta, Carlos Alberto; Tiecher, Tadeu Luis; Lorensini, Felipe; Cancian, Adriana; Stefanello, Lincon; Girotto, Eduardo; Vieira, Renan Costa Beber; Ferreira, Paulo Ademar Avelar; Brunetto, Gustavo

2015-04-01

Successive swine effluent applications can substantially increase the transfer of phosphorus (P) forms in runoff. The aim of this study was to evaluate P accumulation in the soil and transfer of P forms in surface runoff from a Hapludalf soil under no-tillage subjected to successive swine effluent applications. This research was carried out in the Agricultural Engineering Department of the Federal University of Santa Maria, Brazil, from 2004 to 2007, on a Typic Hapludalf soil. Swine effluent rates of 0, 20, 40, and 80 m3 ha(-1) were broadcast over the soil surface prior to sowing of different species in a crop rotation. Soil samples were collected in stratified layers, and the levels of available P were determined. Samples of water runoff from the soil surface were collected throughout the period, and the available, soluble, particulate, and total P were measured. Successive swine effluent applications led to increases in P availability, especially in the soil surface, and P migration through the soil profile. Transfer of P forms was closely associated with runoff, which is directly related to rainfall volume. Swine effluent applications also reduced surface runoff. These results show that in areas with successive swine effluent applications, practices that promote higher water infiltration into the soil are required, e.g., crop rotation and no-tillage system.

Water reuse at highway rest areas : evaluation phase.

DOT National Transportation Integrated Search

1977-01-01

The limited availability of water and stringent wastewater effluent standards at rest areas led to the development of a water recycle-reuse system to treat flush water from water closets. Flush fluid for rest area water closets accounts for 95% to 97...

Organic absorption gas-fired residential heat pump

NASA Astrophysics Data System (ADS)

Murphy, K. P.

The development program of a system utilizing a new absorption pair, R133a (CF3CH2Cl) as the refigerant, and ETFE (ethyletra-hydrofurfury lether) as the absorber fluid, is described. A diagram of the basic configuration is shown. The cooling mode and the heating mode are discussed. Six units of an early hardware design were constructed and tested. Two of these units were placed in home heating service during the 1980-81 season. A market evaluation of the business potential of the absorption system was made, identifying location and size of the likely market for such a system. A performance simulation analysis was performed for seven cities in the US. From these, general characteristics of the areas having the greatest performance benefits were established.

40 CFR 421.302 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2011 CFR

2011-07-01

...) Chlorination off-gas wet air pollution control. BPT Limitations for the Primary and Secondary Titanium... per million pounds) of TiCl4 produced Chromium (total) 0.412 0.168 Lead 0.393 0.187 Nickel 1.797 1.189... Within the range of 7.5 to 10.0 at all times. (b) Chlorination area-vent wet air pollution control. BPT...

40 CFR 421.302 - Effluent limitations guidelines representing the degree of effluent reduction attainable by the...

Code of Federal Regulations, 2010 CFR

2010-07-01

...) Chlorination off-gas wet air pollution control. BPT Limitations for the Primary and Secondary Titanium... per million pounds) of TiCl4 produced Chromium (total) 0.412 0.168 Lead 0.393 0.187 Nickel 1.797 1.189... Within the range of 7.5 to 10.0 at all times. (b) Chlorination area-vent wet air pollution control. BPT...

40 CFR 466.31 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2014 CFR

2014-07-01

... Chromium 16.34 6.32 6.63 2.56 Lead 5.84 2.26 5.06 1.96 Nickel 54.85 21.21 38.90 15.04 Zinc 51.73 20.01 21... ft2 of area processed or coated Chromium 3.35 1.30 1.37 0.53 Lead 1.20 0.47 1.04 0.40 Nickel 11.24 4...

40 CFR 466.31 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2011 CFR

2011-07-01

... Chromium 16.34 6.32 6.63 2.56 Lead 5.84 2.26 5.06 1.96 Nickel 54.85 21.21 38.90 15.04 Zinc 51.73 20.01 21... ft2 of area processed or coated Chromium 3.35 1.30 1.37 0.53 Lead 1.20 0.47 1.04 0.40 Nickel 11.24 4...

40 CFR 466.31 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2012 CFR

2012-07-01

... Chromium 16.34 6.32 6.63 2.56 Lead 5.84 2.26 5.06 1.96 Nickel 54.85 21.21 38.90 15.04 Zinc 51.73 20.01 21... ft2 of area processed or coated Chromium 3.35 1.30 1.37 0.53 Lead 1.20 0.47 1.04 0.40 Nickel 11.24 4...

40 CFR 466.31 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2013 CFR

2013-07-01

... Chromium 16.34 6.32 6.63 2.56 Lead 5.84 2.26 5.06 1.96 Nickel 54.85 21.21 38.90 15.04 Zinc 51.73 20.01 21... ft2 of area processed or coated Chromium 3.35 1.30 1.37 0.53 Lead 1.20 0.47 1.04 0.40 Nickel 11.24 4...

40 CFR 466.31 - Effluent limitations representing the degree of effluent reduction attainable by the application...

Code of Federal Regulations, 2010 CFR

2010-07-01

... Chromium 16.34 6.32 6.63 2.56 Lead 5.84 2.26 5.06 1.96 Nickel 54.85 21.21 38.90 15.04 Zinc 51.73 20.01 21... ft2 of area processed or coated Chromium 3.35 1.30 1.37 0.53 Lead 1.20 0.47 1.04 0.40 Nickel 11.24 4...

Genetics Home Reference: glutaric acidemia type II

MedlinePlus

... E, Bross P, Skovby F, Gregersen N. Clear relationship between ETF/ETFDH genotype and phenotype in patients ... for Links Data Files & API Site Map Subscribe Customer Support USA.gov Copyright Privacy Accessibility FOIA Viewers & ...

Electromagnetically Tunable Fields

DTIC Science & Technology

2008-07-01

constitutive material properties (electrical permittivity, magnetic permeability, and electrical conductivity) of electromagnetically tunable fluids ( ETFs ... trade -offs and operational perspectives of a dielectric coated spherical inverted-F antenna," accepted for IEEE/URSI Int. Symp. Antennas and Propag

76 FR 43206 - Electronic Tariff Filing System (ETFS)

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-20

...) Working papers and statistical data. (1) Concurrently with the filing of any tariff change or tariff... explanation of how the working papers relate to that information. (2) All statistical studies must be...

Particulate contamination from siliconized rubber closures for freeze drying.

PubMed

Gebhardt, U; Grumbridge, N A; Knoch, A

1996-01-01

It can be shown that siliconized closures for freeze drying may cause the opalescence and turbidity observed in freeze-dried products after reconstitution. Closures of different rubber composition show different intensities of turbidity when treated identically with the same quantity and type of silicone oil. Clear solutions are obtained after reconstitution if ETFE-coated closures are used instead of siliconized closures. Samples stored at 4 degrees C for up to 6 months show no change in the intensity of turbidity, while the turbidity of samples manufactured with siliconized closures and stored at higher temperatures increase with time. Samples with ETFE-coated closures show clear solutions when stored at 25 degrees C and 37 degrees C for up to 6 months and at 45 degrees C for 3 months. After 6 months only a very weak opalescence could be observed in these samples.

Manufacturing Methods and Technology Project Execution Report

DTIC Science & Technology

1981-08-01

DISTRIBUTED. 77 3183 IMPROVED PROCESSES FOR INERTIAL GRADE Q-FLEX ACCELERDMETER SuNSTRAND IMPROVED ACCELEROMETER blAS STABILITY, BY THICKENING GOLD ...O h- ^O UJ —« X -H 1— —i 0 □ LU UO U. 1-0 < a. O 3 LU <£ LU O a LU LO C 0 0 LU O < etf <t X) 1/1 ^ O + ^JJ 3 <I to cL • r-J 3 ■L. LU a: a ^ z < 0...x 2 X O Etf a: >- • Q- aC Q_ ct Q- at :£ ^ Q -^ Ct a C^ £ UJ at J at ac UJ J a a kU a. m —" < X ci; _J ac LU Wl i— O ^- —) <o "O i/J to Ml LU if! O

Flexible Pipes-Permeation of Methane, Carbon Dioxide and Water Through Tefzel ETFE: Experiments 1996

NASA Technical Reports Server (NTRS)

Wang, Per Arne; Hydro, Norsk

1997-01-01

The permeation of a mixture of CH4 and CO2 (97% CH4 and 3% CO2) saturated with water vapour through Tefzel has been studied at 950 C and 25 and 50 bars. Tefzel is the Du Pont trademark of an ETFE (ethylenetetrafluorethylene) which is a copolymer of ethylene and tetrafluorethylene. This material might be used as inner plastic lining of flexible pipes. For methane and carbon dioxide, the permeability of Tefzel is higher than the deplasticized PVDF (Polyvinylidenefluoride), but lower than the plasticized PVDF. For water, the situation seems to be the other way round; Tefzel has a lower permeability than deplasticized PVDF. Whether the permeability tests on Tefzel at higher temperatures and pressures will be pursued or not, will be considered by the steering committee of the CAPP project in May.

Temperature dependences of internal friction and shear modulus in glass-textolites irradiated with electrons

NASA Astrophysics Data System (ADS)

Zaikin, Yu. A.; Kozhamkulov, B. A.; Koztaeva, U. P.

1997-07-01

A study is made of mechanical relaxation mechanisms and the correlation between parameters characterizing the temperature dependence of internal friction and shear modulus when the mechanical and electrical properties of glass-textolites of grades ST-11 and ST-ETF are altered by exposure to different doses of high-energy electrons. High-temperature α- and α'- transformation are observed, these transformations being due to the unfreezing of segmental mobility in the polymer matrix and the boundary layers at the surfaces of the glass fibers under the influence of the radiation. A discussion is presented of features of radiation-induced degradation processes in the polymer binder and at points where it contacts the filler. The data that is obtained shows that glass-texolites ST-ETF and ST-11 are highly resistant to radiation.

Tensile properties in collagen-rich tissues of Quarter Horses with hereditary equine regional dermal asthenia (HERDA).

PubMed

Bowser, J E; Elder, S H; Pasquali, M; Grady, J G; Rashmir-Raven, A M; Wills, R; Swiderski, C E

2014-03-01

Hereditary equine regional dermal asthenia (HERDA) is an autosomal recessive disorder of Quarter Horses characterised by skin fragility. Horses with HERDA have a missense mutation in peptidyl-prolyl cis-trans isomerase B (PPIB), which encodes cyclophilin B and alters folding and post translational modifications of fibrillar collagen. The study aimed to test the hypothesis that tendons, ligaments and great vessels, which, like skin, are rich in fibrillar collagen, will also have abnormal biomechanical properties in horses with HERDA. Ex vivo biomechanical study comparing horses with and without a diagnosis of HERDA. Forelimb suspensory ligament, superficial and deep digital flexor tendons; withers, forelimb and abdominal skin; the main pulmonary artery and the aortic arch were harvested from 6 horses with HERDA and 6 control horses without the HERDA allele. Tissues were distracted to failure. Tensile strength (TS), elastic modulus (EM) and energy to failure (ETF) were compared. Horses with HERDA had significantly lower TS and EM in tendinoligamentous tissues and great vessels, respectively. The TS, EM and ETF were significantly lower in skin from horses with HERDA. Differences in TS and ETF were more extreme at the withers than at the forelimb or abdomen. Tendinoligamentous tissue, great vessels and skin are significantly weaker in horses with HERDA than in horses lacking the PPIB mutation, substantiating that diverse tissues with high fibrillar collagen content are abnormal in HERDA and that the HERDA phenotype is not limited to the integument. © 2013 EVJ Ltd.

[Absence of chemical interaction between short catheters (ETFE) and three major antibiotics. Study model].

PubMed

Minosio, J P; Bourget, P; Clapeau, G; Rieutord, A; Hamon, M

1994-01-01

Recourse to sounding with vein catheters is more and more frequent in hospital environments. At the same time, a perceptible increase in incidents and accidents linked to this constantly growing practice is noted. The multiplication of new biomaterials used in the composition of catheters leads to taking into account the criteria of innocuousness and physiochemical inertia as discriminant elements in the choice of biomaterials. A study in vitro has been undertaken of the interaction between short catheters made of ethylenetetrafluoroethylen (ETFE) and antibiotic solutions widely used in hospital environments. The confrontation concerned solutions of vancomycine (Vancocin), ciprofloxacine (Ciflox) and the amoxicillin-clavulanic acid (Augmentin) association. A device has been fitted up and operative conditions have been set in order that the flushing out of the catheters by the solutions be in quality and quantity, as near as possible to actual conditions of use. The interaction marked chosen being a possible release of fluorides ions by the polymer, the determination of this anion has been made by liquid-gas chromatography paired with a flame ionization detection. We show the inertia of ETFE catheters with respect to the solutions examined. Taking into account the initially defined objectives, the conclusion of the work is important and constitutes a considerable factor of security for the catheters user whether he be buyer or practitioner. The model of study making up this approach could very be applied to other categories of materials and therapeutics.

Superamphiphobic and Electroactive Nanocomposite toward Self-Cleaning, Antiwear, and Anticorrosion Coatings.

PubMed

Yuan, Ruixia; Wu, Shiqi; Yu, Peng; Wang, Baohui; Mu, Liwen; Zhang, Xiguang; Zhu, Yixing; Wang, Bing; Wang, Huaiyuan; Zhu, Jiahua

2016-05-18

Multifunctional coatings are in urgent demand in emerging fields. In this work, nanocomposite coatings with extraordinary self-cleaning, antiwear, and anticorrosion properties were prepared on aluminum substrate by a facile spraying technique. Core-shell structured polyaniline/functionalized carbon nanotubes (PANI/fCNTs) composite and nanosized silica were synergistically integrated into ethylene tetrafluoroethylene (ETFE) matrix to construct lotus-leaf-like structures, and 1H,1H,2H,2H- perfluorooctyltriethoxysilane (POTS) was used to decrease the surface energy. The composite coating with 6 wt % PANI/fCNTs possesses superamphiphobic property, with contact angles of 167°, 163°, and 159° toward water, glycerol, and ethylene glycol, respectively. This coating demonstrates stable nonwetting performance over a wide temperature range (<400 °C), as well as outstanding self-cleaning ability to prevent contamination by sludge, concentrated H2SO4, and ethylene glycol. Superamphiphobic surface property could be maintained even after 45 000 times abrasion or bending test for 30 times. The coating displayed strong adhesive ability (grade 1 according to the GB/T9286) on the etched aluminum plate. The superamphiphobic surface could be retained after immersion in 1 mol/L HCl and 3.5 wt % NaCl solutions for 60 and 90 d, respectively. It should be noted that this coating reveals significantly improved anticorrosion performance as compared to the bare ETFE coating and ETFE composite coating without PANI/fCNTs. Such coatings with integrated functionalities offer promising self-cleaning and anticorrosion applications under erosive/abrasive environment.

Properties of r-process nuclei near N=82 shell closure

NASA Astrophysics Data System (ADS)

Farhan, A. R.; Sharma, M. M.

2004-10-01

We have studied properties of nuclei in r-process region near N=82 shell closure with the RMF calculations in a deformed basis using the force NL-SV1 that includes vector self-coupling of w meson. It is shown that nuclei above N=82 in several isotopic chains in the r-process region exhibit an onset of deformation beyond the drip line. Consequently, induced by the deformation these nuclei show an extra stability above the shell closure. This stability of nuclei is expected to contribute to the r-process nucleosynthesis of nuclei below the abundance peak at A ˜130. A comparison with the mass formulae shows that our microscopic calculations with NL-SV1 show a decrease of shell strength with increase in isospin. This is in contrast to the strong shell effects shown by FRDM and ETF-SI in going to the drip line. The stiffness of the shell structure with FRDM and ETF-SI is known to lead to a shortfall in the r-process abundances. This shortcoming of the above mass formulae has inspired an ad-hoc inclusion of shell quenching in the mass formula ETF-SI(Q) with a view to better reproduce the r-process abundances. In comparison, our model shows a decrease of the shell strength in going from the r-process path to the drip line. Therefore, this represents a natural behaviour as required by r-process abundances. It may, however, be confirmed in network chain calculations using inputs from our microscopic model.

Temporal and spatial changes of microbial community in an industrial effluent receiving area in Hangzhou Bay.

PubMed

Zhang, Yan; Chen, Lujun; Sun, Renhua; Dai, Tianjiao; Tian, Jinping; Zheng, Wei; Wen, Donghui

2016-06-01

Anthropogenic activities usually contaminate water environments, and have led to the eutrophication of many estuaries and shifts in microbial communities. In this study, the temporal and spatial changes of the microbial community in an industrial effluent receiving area in Hangzhou Bay were investigated by 454 pyrosequencing. The bacterial community showed higher richness and biodiversity than the archaeal community in all sediments. Proteobacteria dominated in the bacterial communities of all the samples; Marine_Group_I and Methanomicrobia were the two dominant archaeal classes in the effluent receiving area. PCoA and AMOVA revealed strong seasonal but minor spatial changes in both bacterial and archaeal communities in the sediments. The seasonal changes of the bacterial community were less significant than those of the archaeal community, which mainly consisted of fluctuations in abundance of a large proportion of longstanding species rather than the appearance and disappearance of major archaeal species. Temperature was found to positively correlate with the dominant bacteria, Betaproteobacteria, and negatively correlate with the dominant archaea, Marine_Group_I; and might be the primary driving force for the seasonal variation of the microbial community. Copyright © 2016. Published by Elsevier B.V.

Effect of mercury and arsenic from industrial effluents on the drinking water and comparison of the water quality of polluted and non-polluted areas: a case study of Peshawar and Lower Dir.

PubMed

Ishaq, M; Jan, F Akbar; Khan, Murad Ali; Ihsanullah, I; Ahmad, I; Shakirullah, M; Roohullah

2013-02-01

The purpose of the present study was to find out the sources of mercury and arsenic pollution of water in the industrial area of Peshawar, the capital of Khyber Pakhtunkhwa, Pakistan. Samples of effluents, mud, and water were collected from the target area (industrial area of Peshawar), the area of water supply source, and from the less polluted area, the Lower Dir district, as the control. Hg was determined by the cold vapor generation technique, while arsenic was determined using the electrothermal atomic absorption technique. Data of the water from the industrial area were compared with that of the source area, control area, as well as with the WHO and some international drinking water quality standards. The results show that some parameters, i.e., TDS, DO, pH, and hardness, were more than the permissible limits. Textile and glass industries were found to be the major sources of Hg and As pollution. Downstream dilution of these contaminants was also observed.

Fast microbial reduction of ferrihydrite colloids from a soil effluent

NASA Astrophysics Data System (ADS)

Fritzsche, Andreas; Bosch, Julian; Rennert, Thilo; Heister, Katja; Braunschweig, Juliane; Meckenstock, Rainer U.; Totsche, Kai U.

2012-01-01

Recent studies on the microbial reduction of synthetic iron oxide colloids showed their superior electron accepting property in comparison to bulk iron oxides. However, natural colloidal iron oxides differ in composition from their synthetic counterparts. Besides a potential effect of colloid size, microbial iron reduction may be accelerated by electron-shuttling dissolved organic matter (DOM) as well as slowed down by inhibitors such as arsenic. We examined the microbial reduction of OM- and arsenic-containing ferrihydrite colloids. Four effluent fractions were collected from a soil column experiment run under water-saturated conditions. Ferrihydrite colloids precipitated from the soil effluent and exhibited stable hydrodynamic diameters ranging from 281 (±146) nm in the effluent fraction that was collected first and 100 (±43) nm in a subsequently obtained effluent fraction. Aliquots of these oxic effluent fractions were added to anoxic low salt medium containing diluted suspensions of Geobacter sulfurreducens. Independent of the initial colloid size, the soil effluent ferrihydrite colloids were quickly and completely reduced. The rates of Fe2+ formation ranged between 1.9 and 3.3 fmol h-1 cell-1, and are in the range of or slightly exceeding previously reported rates of synthetic ferrihydrite colloids (1.3 fmol h-1 cell-1), but greatly exceeding previously known rates of macroaggregate-ferrihydrite reduction (0.07 fmol h-1 cell-1). The inhibition of microbial Fe(III) reduction by arsenic is unlikely or overridden by the concurrent enhancement induced by soil effluent DOM. These organic species may have increased the already high intrinsic reducibility of colloidal ferrihydrite owing to quinone-mediated electron shuttling. Additionally, OM, which is structurally associated with the soil effluent ferrihydrite colloids, may also contribute to the higher reactivity due to increasing solubility and specific surface area of ferrihydrite. In conclusion, ferrihydrite colloids from soil effluents can be considered as highly reactive electron acceptors in anoxic environments.

Integrating biological treatment of crop residue into a hydroponic sweetpotato culture

NASA Astrophysics Data System (ADS)

Trotman, A. A.; David, P. P.; Bonsi, C. K.; Hill, W. A.; Mortley, D. G.; Loretan, P. A.

1997-01-01

Residual biomass from hydroponic culture of sweetpotato [Ipomoea batatas (L.) Lam.] was degraded using natural bacterial soil isolates. Sweetpotato was grown for 120 days in hydroponic culture with a nutrient solution comprised of a ratio of 80% modified half Hoagland solution to 20% filtered effluent from an aerobic starch hydrolysis bioreactor. The phytotoxicity of the effluent was assayed with `Waldmann's Green' lettuce (Lactuca sativa L.) and the ratio selected after a 60-day bioassay using sweetpotato plants propagated vegetatively from cuttings. Controlled environment chamber experiments were conducted to investigate the impact of filtrate from biological treatment of crop residue on growth and storage root production with plants grown in a modified half Hoagland solution. Incorporation of bioreactor effluent, reduced storage root yield of `Georgia Jet' sweetpotato but the decrease was not statistically significant when compared with yield for plants cultured in a modified half Hoagland solution without filtrate. However, yield of `TU-82-155' sweetpotato was significantly reduced when grown in a modified half Hoagland solution into which filtered effluent had been incorporated. Total biomass was significantly reduced for both sweetpotato cultivars when grown in bioreactor effluent. The leaf area and dry matter accumulation were significantly (P < 0.05) reduced for both cultivars when grown in solution culture containing 20% filtered effluent.

CRADA Payment Options | NCI Technology Transfer Center | TTC

Cancer.gov

NCI TTC CRADA PAYMENT OPTIONS: Electronic Payments by Wire Transfer via Fedwire, Mail a check to the Institute or Center, or Automated Clearing House (ACH)/Electronic Funds Transfer (ETF) payments via Pay.gov (NCI ONLY).

Influence of diligent disintegration on anaerobic biomass and performance of microbial fuel cell.

PubMed

Divyalakshmi, Palanisamy; Murugan, Devaraj; Rai, Chockalingam Lajapathi

2017-12-01

To enhance the performance of microbial fuel cells (MFC) by increasing the surface area of cathode and diligent mechanical disintegration of anaerobic biomass. Tannery effluent and anaerobic biomass were used. The increase in surface area of the cathode resulted in 78% COD removal, with the potential, current density, power density and coulombic efficiency of 675 mV, 147 mA m -2 , 33 mW m -2 and 3.5%, respectively. The work coupled with increased surface area of the cathode with diligent mechanical disintegration of the biomass, led to a further increase in COD removal of 82% with the potential, current density, power density and coulombic efficiency of 748 mV, 229 mA m -2 , 78 mW m -2 and 6% respectively. Mechanical disintegration of the biomass along with increased surface area of cathode enhances power generation in vertical MFC reactors using tannery effluent as fuel.

Assessment of the impact of textile effluents on microbial diversity in Tirupur district, Tamil Nadu

NASA Astrophysics Data System (ADS)

Prabha, Shashi; Gogoi, Anindita; Mazumder, Payal; Ramanathan, AL.; Kumar, Manish

2017-09-01

The expedited advent of urbanization and industrialization for economic growth has adversely affected the biological diversity, which is one of the major concerns of the developing countries. Microbes play a crucial role in decontaminating polluted sites and degrades pollution load of textile effluent. The present study was based on identification of microbial diversity along the Noyaal river of Tirupur area. River water samples from industrial and non-industrial sites and effluent samples of before and after treatment were tested and it was found that microbial diversity was higher in the river water at the industrial site (Kasipalayam) as compared to the non-industrial site (Perur). Similarly, the microbial populations were found to be high in the untreated effluent as compared to the treated one by conventional treatment systems. Similar trends were observed for MBR treatment systems as well. Pseudomonas sp ., Achromobacter sp. (bacterial species) and Aspergillus fumigates (fungal species), found exclusively at the industrial site have been reported to possess decolorization potential of dye effluent, thus can be used for treatment of dye effluent. The comparison of different microbial communities from different dye wastewater sources and textile effluents was done, which showed that the microbes degrade dyestuffs, reduce toxicity of wastewaters, etc. From the study, it can be concluded that the microbial community helps to check on the pollutants and minimize their affect. Therefore, there is a need to understand the systematic variation in microbial diversity with the accumulation of pollution load through monitoring.

Growth of Dalbergia sissoo in desert regions of western India using municipal effluent and the subsequent changes in soil and plant chemistry.

PubMed

Singh, G; Bhati, M

2005-06-01

Increasing demand for fodder and fuelwood and the scarcity of a good quality water in arid areas has resulted in a search for an alternative source of water for biomass production. An experiment utilizing municipal effluent in growing Dalbergia sissoo was conducted. Five treatments included T1, municipal effluent at 1 PET (Potential evapo-transpiration) (without plant); T2, municipal effluent at 1/2 PET; T3, municipal effluent at 1PET; T4, municipal effluent at 2 PET; and T5, canal water at 1 PET. Observations included plant height, collar diameter at one-month intervals and plant mineral composition, mineral uptake and changes in soil properties at 24 months of plant age. Application of municipal effluent produced better growth in D. sissoo seedlings. Concentrations of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), iron (Fe), manganese (Mn) and zinc (Zn) were greater in seedlings irrigated with municipal effluent than those of the seedlings irrigated by the treatment T5, and positively related with the quantity of irrigation. The concentrations were greatest in foliage compared to the other parts of seedling, with the exception of Cu concentration. Application of municipal effluents resulted in a 2- to 3-fold increase in the concentrations of soil K, Cu, Fe, Mn and Zn, whereas NH4-N and PO4-P availability increased by 8.1- and 4.5-fold, respectively. The increase in soil organic carbon was only observed in treatments T3 and T4. The accumulations of soil NO3-N, Na, Cu, Fe, Mn and Zn were more in lower soil layers but the other soil parameters showed their greatest values in the upper soil layer. Irrigation using municipal effluent did not result in toxicity to the seedlings before the age of 24 months. The results suggest that municipal effluent could be utilized, as an important source of water and nutrients in growing D. sissoo to increase biomass production in the needs of suburban dwellers. However, a preliminary treatment to reduce excess NH4-N and PO4-P will be required before application to the plantation.

An assessment of the performance of municipal constructed wetlands in Ireland.

PubMed

Hickey, Anthony; Arnscheidt, Joerg; Joyce, Eadaoin; O'Toole, James; Galvin, Gerry; O' Callaghan, Mark; Conroy, Ken; Killian, Darran; Shryane, Tommy; Hughes, Francis; Walsh, Katherine; Kavanagh, Emily

2018-03-15

While performance assessments of constructed wetlands sites around the world have appraised their capacity for effective removal of organics, a large variance remains in these sites' reported ability to retain nutrients, which appears to depend on differences in design, operation and climate factors. Nutrient retention is a very important objective for constructed wetlands, to avoid eutrophication of aquatic environments receiving their effluents. This study assessed the performance of constructed wetlands in terms of nutrient retention and associated parameters under the humid conditions of Ireland's temperate maritime climate. A review of the performance of 52 constructed wetland sites from 17 local authorities aimed to identify the best performing types of constructed wetlands and the treatment factors determining successful compliance with environmental standards. Data analysis compared effluent results from constructed wetlands with secondary free surface flow or tertiary horizontal subsurface flow, hybrid systems and integrated constructed wetlands with those from small-scale mechanical wastewater treatment plants of the same size class. Nutrient concentrations in effluents of constructed wetlands were negatively correlated (p < .01) with specific area, i.e. the ratio of surface area and population equivalents. The latest generation of integrated constructed wetlands, which had applied design guidelines issued by the Department of the Environment, performed best. Storm management design features improved treatment performance of constructed wetlands significantly (p < .05) for total suspended solids concentrations and exceedance frequency of limit values for total nitrogen. Mechanical wastewater treatment plants, secondary free surface water and tertiary horizontal subsurface flow wetlands showed a very large variance in effluent concentrations for organic and nutrient parameters. E. coli numbers in effluents were lowest for integrated constructed wetlands with an arithmetic mean of 89 MPN/100 ml. Despite Ireland's humid climate, some constructed wetland sites achieved long or frequent periods of zero effluent discharge and thus did not transfer any waterborne pollution to their receptors during these periods. Copyright © 2018 Elsevier Ltd. All rights reserved.

Acoustic profiles and images of the Palos Verdes Margin: Implications concerning deposition from the White's Point outfall

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hampton, M A.; Karl, H; Murray, Christopher J.

2001-12-01

Subbottom profiles and sidescan-sonar images collected on and around the Palos Verdes shelf show a surficial deposit interpreted to contain effluent from the White's Point diffusers, as well as showing several geologic features that affect the deposit's distribution. The effluent-affected deposit is visible in high-resolution subbottom profiles on the shelf and the adjacent San Pedro basin slope to water depths of 170 m. It has a maximum thickness of 75 cm and was mapped acoustically over an area of 10.8 km{sup 2}, which encompasses a volume of about 3.2 million m{sup 3}. The deposit's basal reflector is acoustically distinct overmore » most of the mapped area, implying that the deposit has not been extensively mixed across its base, perhaps being relatively free of reworking since its initial deposition. Nearshore, the basal reflector is weak and fades away toward land, which could result from syndepositional intermixing of coarse native sediment (particularly from the Portuguese Bend landslide) with effluent in the high-energy nearshore zone, or postdepositionally by physical (wave) or biological mixing across the interface. The geometry of the deposit implies that effluent is dispersed primarily in a northwesterly and seaward direction from the diffusers. Dispersal across the shelf break is in some places strongly affected by topography, particularly by submarine canyons. The deposit overlies stratified and unstratified Quaternary sediment, up to 30 m thick, that in turn overlies the irregular erosional surface of deformed Miocene bedrock that crops out in places on the shelf and upper basin slope. The effluent-affected deposit rests on potentially unstable landslide deposits on the San Pedro basin slope. The acoustic profiles and side-scan images show evidence for active and inactive vents, probably of hot water and gas, some of which are within the boundary of the effluent-affected sediment deposit and could disrupt it if seepage occurs.« less

Correlation between DNAPL distribution area and dissolved concentration in surfactant enhanced aquifer remediation effluent: a two-dimensional flow cell study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu, Bin; Li, Huiying; Du, Xiaoming

2016-02-01

During the process of surfactant enhanced aquifer remediation (SEAR), free phase dense non-aqueous phase liquid (DNAPL) may be mobilized and spread. The understanding of the impact of DNAPL spreading on the SEAR remediation is not sufficient with its positive effect infrequently mentioned. To evaluate the correlation between DNAPL spreading and remediation efficiency, a two-dimensional sandbox apparatus was used to simulate the migration and dissolution process of 1,2-DCA (1,2-dichloroethane) DNAPL in SEAR. Distribution area of DNAPL in the sandbox was determined by digital image analysis and correlated with effluent DNAPL concentration. The results showed that the effluent DNAPL concentration has significantmore » positive linear correlation with the DNAPL distribution area, indicating the mobilization of DNAPL could improve remediation efficiency by enlarging total NAPL-water interfacial area for mass transfer. Meanwhile, the vertical migration of 1,2-DCA was limited within the boundary of aquifer in all experiments, implying that by manipulating injection parameters in SEAR, optimal remediation efficiency can be reached while the risk of DNAPL vertical migration is minimized. This study provides a convenient visible and quantitative method for the optimization of parameters for SEAR project, and an approach of rapid predicting the extent of DNAPL contaminant distribution based on the dissolved DNAPL concentration in the extraction well.« less

GREENPLEX -- A SUSTAINABLE URBAN FORM FOR THE 21ST CENTURY

EPA Science Inventory

Outputs include images of architecture, space usage, social design, elevators, skybridges, ETFE envelope, structures, construction process, HVAC system, and water system.  Outputs include performance metrics for the University Community Greenplex and traditional univer...

75 FR 50015 - Self-Regulatory Organizations; International Securities Exchange, LLC; Notice of Filing and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-16

... Solar, Inc. (``FSLR''), Market Vectors ETF Gold Miners (``GDX''), SPDR Gold Trust (``GLD''), iShares DJ... still lower than fees charged by other options exchanges. PHLX, For example, currently charges Broker...

Kinetics and characterization of radiation-induced grafting of styrene on fluoropolymers

NASA Astrophysics Data System (ADS)

Guilmeau, I.; Esnouf, S.; Betz, N.; Le Moël, A.

1997-08-01

Grafting of styrene solution onto poly(ethylene-co-tetrafluoroethylene) (ETFE) was carried out by the pre-irradiation method. ETFE films were irradiated by 1.5 MeV electron beams in air. The influence of grafting temperature (50 to 80°C) has been investigated. It was found that the saturation grafting yield and the initial rate follow an Arrhenius law. The volume grafting yields were measured by FTIR spectroscopy in transmission and by weighing and the 'surface' grafting yields by FTIR-ATR. The results showed that grafting reaction is not monomer diffusion controlled in 30 μm film, nevertheless heterogeneities are revealed. By in-situ ESR, the decay of peroxy radicals was recorded under various heating and grafting conditions. These experiments suggest that the peroxy radicals react rapidly with monomer, but do not initiate the grafting process. The propagating radicals were not detectable, which may indicate that polystyrene chains are very long.

Fusion of spectral models for dynamic modeling of sEMG and skeletal muscle force.

PubMed

Potluri, Chandrasekhar; Anugolu, Madhavi; Chiu, Steve; Urfer, Alex; Schoen, Marco P; Naidu, D Subbaram

2012-01-01

In this paper, we present a method of combining spectral models using a Kullback Information Criterion (KIC) data fusion algorithm. Surface Electromyographic (sEMG) signals and their corresponding skeletal muscle force signals are acquired from three sensors and pre-processed using a Half-Gaussian filter and a Chebyshev Type- II filter, respectively. Spectral models - Spectral Analysis (SPA), Empirical Transfer Function Estimate (ETFE), Spectral Analysis with Frequency Dependent Resolution (SPFRD) - are extracted from sEMG signals as input and skeletal muscle force as output signal. These signals are then employed in a System Identification (SI) routine to establish the dynamic models relating the input and output. After the individual models are extracted, the models are fused by a probability based KIC fusion algorithm. The results show that the SPFRD spectral models perform better than SPA and ETFE models in modeling the frequency content of the sEMG/skeletal muscle force data.

Optimized Projection Matrix for Compressive Sensing

NASA Astrophysics Data System (ADS)

Xu, Jianping; Pi, Yiming; Cao, Zongjie

2010-12-01

Compressive sensing (CS) is mainly concerned with low-coherence pairs, since the number of samples needed to recover the signal is proportional to the mutual coherence between projection matrix and sparsifying matrix. Until now, papers on CS always assume the projection matrix to be a random matrix. In this paper, aiming at minimizing the mutual coherence, a method is proposed to optimize the projection matrix. This method is based on equiangular tight frame (ETF) design because an ETF has minimum coherence. It is impossible to solve the problem exactly because of the complexity. Therefore, an alternating minimization type method is used to find a feasible solution. The optimally designed projection matrix can further reduce the necessary number of samples for recovery or improve the recovery accuracy. The proposed method demonstrates better performance than conventional optimization methods, which brings benefits to both basis pursuit and orthogonal matching pursuit.

Extended Thomas-Fermi density functional for the unitary Fermi gas

NASA Astrophysics Data System (ADS)

Salasnich, Luca; Toigo, Flavio

2008-11-01

We determine the energy density ξ(3/5)nɛF and the gradient correction λℏ2(∇n)2/(8mn) of the extended Thomas-Fermi (ETF) density functional, where n is the number density and ɛF is the Fermi energy, for a trapped two-component Fermi gas with infinite scattering length (unitary Fermi gas) on the basis of recent diffusion Monte Carlo (DMC) calculations [Phys. Rev. Lett. 99, 233201 (2007)]. In particular we find that ξ=0.455 and λ=0.13 give the best fit of the DMC data with an even number N of particles. We also study the odd-even splitting γN1/9ℏω of the ground-state energy for the unitary gas in a harmonic trap of frequency ω determining the constant γ . Finally we investigate the effect of the gradient term in the time-dependent ETF model by introducing generalized Galilei-invariant hydrodynamics equations.