DArT Markers Effectively Target Gene Space in the Rye Genome.

PubMed

Gawroński, Piotr; Pawełkowicz, Magdalena; Tofil, Katarzyna; Uszyński, Grzegorz; Sharifova, Saida; Ahluwalia, Shivaksh; Tyrka, Mirosław; Wędzony, Maria; Kilian, Andrzej; Bolibok-Brągoszewska, Hanna

2016-01-01

Large genome size and complexity hamper considerably the genomics research in relevant species. Rye ( Secale cereale L.) has one of the largest genomes among cereal crops and repetitive sequences account for over 90% of its length. Diversity Arrays Technology is a high-throughput genotyping method, in which a preferential sampling of gene-rich regions is achieved through the use of methylation sensitive restriction enzymes. We obtained sequences of 6,177 rye DArT markers and following a redundancy analysis assembled them into 3,737 non-redundant sequences, which were then used in homology searches against five Pooideae sequence sets. In total 515 DArT sequences could be incorporated into publicly available rye genome zippers providing a starting point for the integration of DArT- and transcript-based genomics resources in rye. Using Blast2Go pipeline we attributed putative gene functions to 1101 (29.4%) of the non-redundant DArT marker sequences, including 132 sequences with putative disease resistance-related functions, which were found to be preferentially located in the 4RL and 6RL chromosomes. Comparative analysis based on the DArT sequences revealed obvious inconsistencies between two recently published high density consensus maps of rye. Furthermore we demonstrated that DArT marker sequences can be a source of SSR polymorphisms. Obtained data demonstrate that DArT markers effectively target gene space in the large, complex, and repetitive rye genome. Through the annotation of putative gene functions and the alignment of DArT sequences relative to reference genomes we obtained information, that will complement the results of the studies, where DArT genotyping was deployed, by simplifying the gene ontology and microcolinearity based identification of candidate genes.

Diversity Arrays Technology (DArT) Marker Platforms for Diversity Analysis and Linkage Mapping in a Complex Crop, the Octoploid Cultivated Strawberry (Fragaria × ananassa)

PubMed Central

Sánchez-Sevilla, José F.; Horvath, Aniko; Botella, Miguel A.; Gaston, Amèlia; Folta, Kevin; Kilian, Andrzej; Denoyes, Beatrice; Amaya, Iraida

2015-01-01

Cultivated strawberry (Fragaria × ananassa) is a genetically complex allo-octoploid crop with 28 pairs of chromosomes (2n = 8x = 56) for which a genome sequence is not yet available. The diploid Fragaria vesca is considered the donor species of one of the octoploid sub-genomes and its available genome sequence can be used as a reference for genomic studies. A wide number of strawberry cultivars are stored in ex situ germplasm collections world-wide but a number of previous studies have addressed the genetic diversity present within a limited number of these collections. Here, we report the development and application of two platforms based on the implementation of Diversity Array Technology (DArT) markers for high-throughput genotyping in strawberry. The first DArT microarray was used to evaluate the genetic diversity of 62 strawberry cultivars that represent a wide range of variation based on phenotype, geographical and temporal origin and pedigrees. A total of 603 DArT markers were used to evaluate the diversity and structure of the population and their cluster analyses revealed that these markers were highly efficient in classifying the accessions in groups based on historical, geographical and pedigree-based cues. The second DArTseq platform took benefit of the complexity reduction method optimized for strawberry and the development of next generation sequencing technologies. The strawberry DArTseq was used to generate a total of 9,386 SNP markers in the previously developed ‘232’ × ‘1392’ mapping population, of which, 4,242 high quality markers were further selected to saturate this map after several filtering steps. The high-throughput platforms here developed for genotyping strawberry will facilitate genome-wide characterizations of large accessions sets and complement other available options. PMID:26675207

Accuracy and training population design for genomic selection in elite north american oats

USDA-ARS?s Scientific Manuscript database

Genomic selection (GS) is a method to estimate the breeding values of individuals by using markers throughout the genome. We evaluated the accuracies of GS using data from five traits on 446 oat lines genotyped with 1005 Diversity Array Technology (DArT) markers and two GS methods (RR-BLUP and Bayes...

Development of DArT markers and assessment of diversity in Fusarium oxysporum f. sp. ciceris, wilt pathogen of chickpea (Cicer arietinum L.).

PubMed

Sharma, Mamta; Nagavardhini, Avuthu; Thudi, Mahendar; Ghosh, Raju; Pande, Suresh; Varshney, Rajeev K

2014-06-10

Fusarium oxysporum f. sp. ciceris (Foc), the causal agent of Fusarium wilt of chickpea is highly variable and frequent recurrence of virulent forms have affected chickpea production and exhausted valuable genetic resources. The severity and yield losses of Fusarium wilt differ from place to place owing to existence of physiological races among isolates. Diversity study of fungal population associated with a disease plays a major role in understanding and devising better disease control strategies. The advantages of using molecular markers to understand the distribution of genetic diversity in Foc populations is well understood. The recent development of Diversity Arrays Technology (DArT) offers new possibilities to study the diversity in pathogen population. In this study, we developed DArT markers for Foc population, analysed the genetic diversity existing within and among Foc isolates, compared the genotypic and phenotypic diversity and infer the race scenario of Foc in India. We report the successful development of DArT markers for Foc and their utility in genotyping of Foc collections representing five chickpea growing agro-ecological zones of India. The DArT arrays revealed a total 1,813 polymorphic markers with an average genotyping call rate of 91.16% and a scoring reproducibility of 100%. Cluster analysis, principal coordinate analysis and population structure indicated that the different isolates of Foc were partially classified based on geographical source. Diversity in Foc population was compared with the phenotypic variability and it was found that DArT markers were able to group the isolates consistent with its virulence group. A number of race-specific unique and rare alleles were also detected. The present study generated significant information in terms of pathogenic and genetic diversity of Foc which could be used further for development and deployment of region-specific resistant cultivars of chickpea. The DArT markers were proved to be a powerful diagnostic tool to study the genotypic diversity in Foc. The high number of DArT markers allowed a greater resolution of genetic differences among isolates and enabled us to examine the extent of diversity in the Foc population present in India, as well as provided support to know the changing race scenario in Foc population.

Decision Analysis for Remediation Technologies (DART) user`s manual

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sebo, D.

1997-09-01

This user`s manual is an introduction to the use of the Decision Analysis for Remediation Technology (DART) Report Generator. DART provides a user interface to a database containing site data (e.g., contaminants, waste depth, area) for sites within the Subsurface Contaminant Focus Area (SCFA). The database also contains SCFA requirements, needs, and technology information. The manual is arranged in two major sections. The first section describes loading DART onto a user system. The second section describes DART operation. DART operation is organized into sections by the user interface forms. For each form, user input, both optional and required, DART capabilities,more » and the result of user selections will be covered in sufficient detail to enable the user to understand DART, capabilities and determine how to use DART to meet specific needs.« less

Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

PubMed Central

Vipin, Cina Ann; Luckett, David J.; Harper, John D.I.; Ash, Gavin J.; Kilian, Andrzej; Ellwood, Simon R.; Phan, Huyen T.T.; Raman, Harsh

2013-01-01

We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies. PMID:24273424

A consensus genetic map of sorghum that integrates multiple component maps and high-throughput diversity array technology (DArT) markers

USDA-ARS?s Scientific Manuscript database

This final consensus map has allowed us to map a larger number of markers than possible in any individual map of sorghum, to obtain a more complete coverage of the sorghum genome and to fill a number of gaps on individual maps. In addition to overall general consistency of marker order across indiv...

System description for DART (Decision Analysis for Remediation Technologies)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nonte, J.; Bolander, T.; Nickelson, D.

1997-09-01

DART is a computer aided system populated with influence models to determine quantitative benefits derived by matching requirements and technologies. The DART database is populated with data from over 900 DOE sites from 10 Field Offices. These sites are either source terms, such as buried waste pits, or soil or groundwater contaminated plumes. The data, traceable to published documents, consists of site-specific data (contaminants, area, volume, depth, size, remedial action dates, site preferred remedial option), problems (e.g., offsite contaminant plume), and Site Technology Coordinating Group (STCG) need statements (also contained in the Ten-Year Plan). DART uses this data to calculatemore » and derive site priorities, risk rankings, and site specific technology requirements. DART is also populated with over 900 industry and DOE SCFA technologies. Technology capabilities can be used to match technologies to waste sites based on the technology`s capability to meet site requirements and constraints. Queries may be used to access, sort, roll-up, and rank site data. Data roll-ups may be graphically displayed.« less

New Diversity Arrays Technology (DArT) markers for tetraploid oat (Avena magna Murphy et Terrell) provide the first complete oat linkage map and markers linked to domestication genes from hexaploid A. sativa L.

USDA-ARS?s Scientific Manuscript database

Nutritional benefits of cultivated oat (Avena sativa L., 2n = 6x = 42, AACCDD genomes) are well recognized; however, seed protein levels are modest and genetic resources for protein improvement are scarce. The wild tetraploid A. magna Ladiz. contains approximately 31% seed protein and has been hybr...

KSC-04pd1595

NASA Image and Video Library

2004-07-14

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, an Orbital Sciences technician works with wiring on the DART (Demonstration for Autonomous Rendezvous Technology) flight demonstrator, a spacecraft developed to prove technologies for locating and maneuvering near an orbiting satellite. Future applications of technologies developed by the DART project will benefit the nation in future space-vehicle systems development requiring in-space assembly, services or other autonomous rendezvous operations. Designed and developed for NASA by Orbital Sciences Corporation in Dulles, Va., the DART spacecraft will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1592

NASA Image and Video Library

2004-07-14

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, Orbital Sciences workers remove the canister from the DART (Demonstration for Autonomous Rendezvous Technology) flight demonstrator, a spacecraft developed to prove technologies for locating and maneuvering near an orbiting satellite. Future applications of technologies developed by the DART project will benefit the nation in future space-vehicle systems development requiring in-space assembly, services or other autonomous rendezvous operations. Designed and developed for NASA by Orbital Sciences Corporation in Dulles, Va., the DART spacecraft will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1599

NASA Image and Video Library

2004-07-14

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, Orbital Sciences technicians watch closely as the DART (Demonstration for Autonomous Rendezvous Technology) flight demonstrator is lowered onto a stand. The spacecraft was developed to prove technologies for locating and maneuvering near an orbiting satellite. Future applications of technologies developed by the DART project will benefit the nation in future space-vehicle systems development requiring in-space assembly, services or other autonomous rendezvous operations. Designed and developed for NASA by Orbital Sciences Corporation in Dulles, Va., the DART spacecraft will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1594

NASA Image and Video Library

2004-07-14

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the DART (Demonstration for Autonomous Rendezvous Technology) flight demonstrator is revealed after its protective cover has been removed. The spacecraft was developed to prove technologies for locating and maneuvering near an orbiting satellite. Future applications of technologies developed by the DART project will benefit the nation in future space-vehicle systems development requiring in-space assembly, services or other autonomous rendezvous operations. Designed and developed for NASA by Orbital Sciences Corporation in Dulles, Va., the DART spacecraft will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1593

NASA Image and Video Library

2004-07-14

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the DART (Demonstration for Autonomous Rendezvous Technology) flight demonstrator is revealed after its protective cover has been removed. The spacecraft was developed to prove technologies for locating and maneuvering near an orbiting satellite. Future applications of technologies developed by the DART project will benefit the nation in future space-vehicle systems development requiring in-space assembly, services or other autonomous rendezvous operations. Designed and developed for NASA by Orbital Sciences Corporation in Dulles, Va., the DART spacecraft will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04PD-1593

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Vandenberg Air Force Base in California, the DART (Demonstration for Autonomous Rendezvous Technology) flight demonstrator is revealed after its protective cover has been removed. The spacecraft was developed to prove technologies for locating and maneuvering near an orbiting satellite. Future applications of technologies developed by the DART project will benefit the nation in future space-vehicle systems development requiring in-space assembly, services or other autonomous rendezvous operations. Designed and developed for NASA by Orbital Sciences Corporation in Dulles, Va., the DART spacecraft will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbitals Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1817

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, workers prepare the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft for launch. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Orbital Sciences Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1597

NASA Image and Video Library

2004-07-14

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, Orbital Sciences technicians check the bottom of the DART (Demonstration for Autonomous Rendezvous Technology) flight demonstrator as it is raised of its platform. The spacecraft was developed to prove technologies for locating and maneuvering near an orbiting satellite. Future applications of technologies developed by the DART project will benefit the nation in future space-vehicle systems development requiring in-space assembly, services or other autonomous rendezvous operations. Designed and developed for NASA by Orbital Sciences Corporation in Dulles, Va., the DART spacecraft will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1596

NASA Image and Video Library

2004-07-14

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, Orbital Sciences technicians check the bottom of the DART (Demonstration for Autonomous Rendezvous Technology) flight demonstrator as it is raised off its platform. The spacecraft was developed to prove technologies for locating and maneuvering near an orbiting satellite. Future applications of technologies developed by the DART project will benefit the nation in future space-vehicle systems development requiring in-space assembly, services or other autonomous rendezvous operations. Designed and developed for NASA by Orbital Sciences Corporation in Dulles, Va., the DART spacecraft will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1598

NASA Image and Video Library

2004-07-14

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, Orbital Sciences technicians observe closely the movement of the DART (Demonstration for Autonomous Rendezvous Technology) flight demonstrator as it is lowered onto a stand. The spacecraft was developed to prove technologies for locating and maneuvering near an orbiting satellite. Future applications of technologies developed by the DART project will benefit the nation in future space-vehicle systems development requiring in-space assembly, services or other autonomous rendezvous operations. Designed and developed for NASA by Orbital Sciences Corporation in Dulles, Va., the DART spacecraft will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1819

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, workers help guide the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft onto the mobile stand below. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Orbital Sciences Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

Video-Guidance Design for the DART Rendezvous Mission

NASA Technical Reports Server (NTRS)

Ruth, Michael; Tracy, Chisholm

2004-01-01

NASA's Demonstration of Autonomous Rendezvous Technology (DART) mission will validate a number of different guidance technologies, including state-differenced GPS transfers and close-approach video guidance. The video guidance for DART will employ NASA/Marshall s Advanced Video Guidance Sensor (AVGS). This paper focuses on the terminal phase of the DART mission that includes close-approach maneuvers under AVGS guidance. The closed-loop video guidance design for DART is driven by a number of competing requirements, including a need for maximizing tracking bandwidths while coping with measurement noise and the need to minimize RCS firings. A range of different strategies for attitude control and docking guidance have been considered for the DART mission, and design decisions are driven by a goal of minimizing both the design complexity and the effects of video guidance lags. The DART design employs an indirect docking approach, in which the guidance position targets are defined using relative attitude information. Flight simulation results have proven the effectiveness of the video guidance design.

KSC-04pd1826

NASA Image and Video Library

2004-09-02

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft (right) is ready for mating with the upper stage (foreground) in preparation for launch on the Orbital Sciences Pegasus XL. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1830

NASA Image and Video Library

2004-09-03

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, workers maneuver the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft and mated upper stage toward the second stage at right in preparation or launch aboard the Orbital Sciences Pegasus XL launch vehicle. Pegasus will launch DART into a circular polar orbit of approximately 475 miles. Built for NASA by Orbital Sciences Corporation, DART was designed as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1827

NASA Image and Video Library

2004-09-02

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, workers maneuver the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft, suspended by a crane, over the upper stage in preparation for launch on the Orbital Sciences Pegasus XL. The Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. Built for NASA by Orbital Sciences Corporation, DART was designed as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1820

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft (in background) has been rotated from vertical to horizontal and is ready for mating with the upper stage (foreground). DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Orbital Sciences Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1823

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, workers begin closing the gap between the second and third stages of the Orbital Sciences Pegasus XL launch vehicle that will launch the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA's Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1828

NASA Image and Video Library

2004-09-03

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, workers maneuver the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft and mated upper stage toward the second stage behind them in preparation or launch aboard the Orbital Sciences Pegasus XL launch vehicle. Pegasus will launch DART into a circular polar orbit of approximately 475 miles. Built for NASA by Orbital Sciences Corporation, DART was designed as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1816

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, a worker prepares the second and third stages of the Orbital Sciences Pegasus XL launch vehicle for mating. The Pegasus XL will launch the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1825

NASA Image and Video Library

2004-09-02

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft (right) is ready for mating with the upper stage (behind it) in preparation for launch on the Orbital Sciences Pegasus XL. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1818

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, workers stand by while an overhead crane moves the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft onto the mobile stand at right. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Orbital Sciences Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1821

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft is ready for mating with the upper stage of the Orbital Sciences Pegasus XL behind it (right). DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1822

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, workers begin mating the second and third stages of the Orbital Sciences Pegasus XL launch vehicle that will launch the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA's Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1829

NASA Image and Video Library

2004-09-03

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft (foreground) is ready to be mated to second and third stages in preparation for the launch aboard the Orbital Sciences Pegasus XL launch vehicle. Pegasus will launch DART into a circular polar orbit of approximately 475 miles. Built for NASA by Orbital Sciences Corporation, DART was designed as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA’s Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1815

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, workers prepare to mate the second and third stages of the Orbital Sciences Pegasus XL launch vehicle that will launch the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA's Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1639

NASA Image and Video Library

2004-07-27

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft is on a work stand waiting for processing activities. The spacecraft was developed for NASA by Orbital Sciences Corporation in Dulles, Va., to prove technologies for locating and maneuvering near an orbiting satellite. DART will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1638

NASA Image and Video Library

2004-07-27

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft is placed on a work stand for processing activities. The spacecraft was developed for NASA by Orbital Sciences Corporation in Dulles, Va., to prove technologies for locating and maneuvering near an orbiting satellite. DART will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04PD-1818

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Vandenberg Air Force Base in California, workers stand by while an overhead crane moves the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft onto the mobile stand at right. DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. The Orbital Sciences Pegasus XL will launch DART into a circular polar orbit of approximately 475 miles. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASAs Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04PD-1830

NASA Technical Reports Server (NTRS)

2004-01-01

KENNEDY SPACE CENTER, FLA. At Vandenberg Air Force Base in California, workers maneuver the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft and mated upper stage toward the second stage at right in preparation or launch aboard the Orbital Sciences Pegasus XL launch vehicle. Pegasus will launch DART into a circular polar orbit of approximately 475 miles. Built for NASA by Orbital Sciences Corporation, DART was designed as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. DART weighs about 800 pounds and is nearly 6 feet long and 3 feet in diameter. DART is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASAs Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station.

KSC-04pd1636

NASA Image and Video Library

2004-07-27

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft is raised to a vertical position. It will be lifted onto a test stand for launch processing activities. The spacecraft was developed for NASA by Orbital Sciences Corporation in Dulles, Va., to prove technologies for locating and maneuvering near an orbiting satellite. DART will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

KSC-04pd1637

NASA Image and Video Library

2004-07-27

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft is raised to a vertical position. It will be lifted onto a test stand for launch processing activities. The spacecraft was developed for NASA by Orbital Sciences Corporation in Dulles, Va., to prove technologies for locating and maneuvering near an orbiting satellite. DART will be launched on a Pegasus launch vehicle. At about 40,000 feet over the Pacific Ocean, the Pegasus will be released from Orbital’s Stargazer L-1011 aircraft, fire its rocket motors and boost DART into a polar orbit approximately 472 miles by 479 miles. Once in orbit, DART will rendezvous with a target satellite, the Multiple Paths, Beyond-Line-of-Site Communications satellite, also built by Orbital Sciences. DART will then perform several close proximity operations, such as moving toward and away from the satellite using navigation data provided by onboard sensors. DART is scheduled for launch no earlier than Oct. 18.

A reference linkage map for Eucalyptus

PubMed Central

2012-01-01

Background Genetic linkage maps are invaluable resources in plant research. They provide a key tool for many genetic applications including: mapping quantitative trait loci (QTL); comparative mapping; identifying unlinked (i.e. independent) DNA markers for fingerprinting, population genetics and phylogenetics; assisting genome sequence assembly; relating physical and recombination distances along the genome and map-based cloning of genes. Eucalypts are the dominant tree species in most Australian ecosystems and of economic importance globally as plantation trees. The genome sequence of E. grandis has recently been released providing unprecedented opportunities for genetic and genomic research in the genus. A robust reference linkage map containing sequence-based molecular markers is needed to capitalise on this resource. Several high density linkage maps have recently been constructed for the main commercial forestry species in the genus (E. grandis, E. urophylla and E. globulus) using sequenced Diversity Arrays Technology (DArT) and microsatellite markers. To provide a single reference linkage map for eucalypts a composite map was produced through the integration of data from seven independent mapping experiments (1950 individuals) using a marker-merging method. Results The composite map totalled 1107 cM and contained 4101 markers; comprising 3880 DArT, 213 microsatellite and eight candidate genes. Eighty-one DArT markers were mapped to two or more linkage groups, resulting in the 4101 markers being mapped to 4191 map positions. Approximately 13% of DArT markers mapped to identical map positions, thus the composite map contained 3634 unique loci at an average interval of 0.31 cM. Conclusion The composite map represents the most saturated linkage map yet produced in Eucalyptus. As the majority of DArT markers contained on the map have been sequenced, the map provides a direct link to the E. grandis genome sequence and will serve as an important reference for progressing eucalypt research. PMID:22702473

DART Employees at Work

NASA Image and Video Library

2014-10-31

The Dust Atmospheric Recovery Technology, or DART, spacecraft is being assembled in a laboratory inside the Space Life Sciences Lab at NASA’s Kennedy Space Center in Florida. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces.

DART Employees at Work

NASA Image and Video Library

2014-10-31

A researcher at NASA’s Kennedy Space Center in Florida checks a reading on the Dust Atmospheric Recovery Technology, or DART, spacecraft inside a laboratory at the Space Life Sciences Lab. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces.

DART Employees at Work

NASA Image and Video Library

2014-10-31

Researchers at NASA’s Kennedy Space Center in Florida check readings on the Dust Atmospheric Recovery Technology, or DART, spacecraft inside a laboratory at the Space Life Sciences Lab. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces.

A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis.

PubMed

King, Julie; Thomas, Ann; James, Caron; King, Ian; Armstead, Ian

2013-07-03

Ryegrasses and fescues (genera, Lolium and Festuca) are species of forage and turf grasses which are used widely in agricultural and amenity situations. They are classified within the sub-family Pooideae and so are closely related to Brachypodium distachyon, wheat, barley, rye and oats. Recently, a DArT array has been developed which can be used in generating marker and mapping information for ryegrasses and fescues. This represents a potential common marker set for ryegrass and fescue researchers which can be linked through to comparative genomic information for the grasses. A F2 perennial ryegrass genetic map was developed consisting of 7 linkage groups defined by 1316 markers and deriving a total map length of 683 cM. The marker set included 866 DArT and 315 gene sequence-based markers. Comparison with previous DArT mapping studies in perennial and Italian ryegrass (L. multiflorum) identified 87 and 105 DArT markers in common, respectively, of which 94% and 87% mapped to homoeologous linkage groups. A similar comparison with meadow fescue (F. pratensis) identified only 28 DArT markers in common, of which c. 50% mapped to non-homoelogous linkage groups. In L. perenne, the genetic distance spanned by the DArT markers encompassed the majority of the regions that could be described in terms of comparative genomic relationships with rice, Brachypodium distachyon, and Sorghum bicolor. DArT markers are likely to be a useful common marker resource for ryegrasses and fescues, though the success in aligning different populations through the mapping of common markers will be influenced by degrees of population interrelatedness. The detailed mapping of DArT and gene-based markers in this study potentially allows comparative relationships to be derived in future mapping populations characterised using solely DArT markers.

KSC-04pd1824

NASA Image and Video Library

2004-09-01

KENNEDY SPACE CENTER, FLA. - At Vandenberg Air Force Base in California, Corky Philyaw (left) and Edgar Suarez (right) prepare the flight battery for installation on the Demonstration of Autonomous Rendezvous Technology (DART) spacecraft (far left). DART was designed and built for NASA by Orbital Sciences Corporation as an advanced flight demonstrator to locate and maneuver near an orbiting satellite. It is designed to demonstrate technologies required for a spacecraft to locate and rendezvous, or maneuver close to, other craft in space. Results from the DART mission will aid in the development of NASA's Crew Exploration Vehicle and will also assist in vehicle development for crew transfer and crew rescue capability to and from the International Space Station. DART will be launched from an Orbital Sciences Pegasus XL rocket no earlier than Oct. 26.

A High Density Consensus Map of Rye (Secale cereale L.) Based on DArT Markers

PubMed Central

Myśków, Beata; Stojałowski, Stefan; Heller-Uszyńska, Katarzyna; Góralska, Magdalena; Brągoszewski, Piotr; Uszyński, Grzegorz; Kilian, Andrzej; Rakoczy-Trojanowska, Monika

2011-01-01

Background Rye (Secale cereale L.) is an economically important crop, exhibiting unique features such as outstanding resistance to biotic and abiotic stresses and high nutrient use efficiency. This species presents a challenge to geneticists and breeders due to its large genome containing a high proportion of repetitive sequences, self incompatibility, severe inbreeding depression and tissue culture recalcitrance. The genomic resources currently available for rye are underdeveloped in comparison with other crops of similar economic importance. The aim of this study was to create a highly saturated, multilocus linkage map of rye via consensus mapping, based on Diversity Arrays Technology (DArT) markers. Methodology/Principal Findings Recombinant inbred lines (RILs) from 5 populations (564 in total) were genotyped using DArT markers and subjected to linkage analysis using Join Map 4.0 and Multipoint Consensus 2.2 software. A consensus map was constructed using a total of 9703 segregating markers. The average chromosome map length ranged from 199.9 cM (2R) to 251.4 cM (4R) and the average map density was 1.1 cM. The integrated map comprised 4048 loci with the number of markers per chromosome ranging from 454 for 7R to 805 for 4R. In comparison with previously published studies on rye, this represents an eight-fold increase in the number of loci placed on a consensus map and a more than two-fold increase in the number of genetically mapped DArT markers. Conclusions/Significance Through the careful choice of marker type, mapping populations and the use of software packages implementing powerful algorithms for map order optimization, we produced a valuable resource for rye and triticale genomics and breeding, which provides an excellent starting point for more in-depth studies on rye genome organization. PMID:22163026

Genome Microscale Heterogeneity among Wild Potatoes Revealed by Diversity Arrays Technology Marker Sequences.

PubMed

Traini, Alessandra; Iorizzo, Massimo; Mann, Harpartap; Bradeen, James M; Carputo, Domenico; Frusciante, Luigi; Chiusano, Maria Luisa

2013-01-01

Tuber-bearing potato species possess several genes that can be exploited to improve the genetic background of the cultivated potato Solanum tuberosum. Among them, S. bulbocastanum and S. commersonii are well known for their strong resistance to environmental stresses. However, scant information is available for these species in terms of genome organization, gene function, and regulatory networks. Consequently, genomic tools to assist breeding are meager, and efficient exploitation of these species has been limited so far. In this paper, we employed the reference genome sequences from cultivated potato and tomato and a collection of sequences of 1,423 potato Diversity Arrays Technology (DArT) markers that show polymorphic representation across the genomes of S. bulbocastanum and/or S. commersonii genotypes. Our results highlighted microscale genome sequence heterogeneity that may play a significant role in functional and structural divergence between related species. Our analytical approach provides knowledge of genome structural and sequence variability that could not be detected by transcriptome and proteome approaches.

DART Employees at Work

NASA Image and Video Library

2014-10-31

A researcher from the University of Florida in Gainesville, checks the Dust Atmospheric Recovery Technology, or DART, spacecraft in a laboratory inside the Space Life Sciences Lab at NASA’s Kennedy Space Center in Florida. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces.

DART AVGS Performance

NASA Technical Reports Server (NTRS)

Howard, Richard T.; Bryan, Thomas C.

2007-01-01

The Advanced Video Guidance Sensor (AVGS) was designed to be the proximity operations sensor for the Demonstration of Autonomous Rendezvous Technologies (DART). The DART mission flew in April of2005 and was a partial success. The AVGS did not get the opportunity to operate in every mode in orbit, but those modes in which it did operate were completely successful. This paper will detail the development, testing, and on-orbit performance of the AVGS.

DART: Instrument Package Developed for Investigating Atmospheric Dust on Mars

NASA Technical Reports Server (NTRS)

Landis, Geoffrey A.

2001-01-01

Mars' dust-filled atmosphere could be a significant problem for photovoltaic array operation during long missions on the surface of Mars. Measurements made by Pathfinder showed a 0.3-percent loss of solar array performance per day due to dust obscuration. Thus, dust deposition is the limiting factor in the lifetime of solar arrays for Martian power systems, and developing design tools to mitigate this deposition is important for long missions.

Dynamic autonomous routing technology for IP-based satellite ad hoc networks

NASA Astrophysics Data System (ADS)

Wang, Xiaofei; Deng, Jing; Kostas, Theresa; Rajappan, Gowri

2014-06-01

IP-based routing for military LEO/MEO satellite ad hoc networks is very challenging due to network and traffic heterogeneity, network topology and traffic dynamics. In this paper, we describe a traffic priority-aware routing scheme for such networks, namely Dynamic Autonomous Routing Technology (DART) for satellite ad hoc networks. DART has a cross-layer design, and conducts routing and resource reservation concurrently for optimal performance in the fluid but predictable satellite ad hoc networks. DART ensures end-to-end data delivery with QoS assurances by only choosing routing paths that have sufficient resources, supporting different packet priority levels. In order to do so, DART incorporates several resource management and innovative routing mechanisms, which dynamically adapt to best fit the prevailing conditions. In particular, DART integrates a resource reservation mechanism to reserve network bandwidth resources; a proactive routing mechanism to set up non-overlapping spanning trees to segregate high priority traffic flows from lower priority flows so that the high priority flows do not face contention from low priority flows; a reactive routing mechanism to arbitrate resources between various traffic priorities when needed; a predictive routing mechanism to set up routes for scheduled missions and for anticipated topology changes for QoS assurance. We present simulation results showing the performance of DART. We have conducted these simulations using the Iridium constellation and trajectories as well as realistic military communications scenarios. The simulation results demonstrate DART's ability to discriminate between high-priority and low-priority traffic flows and ensure disparate QoS requirements of these traffic flows.

Development of DArT-based PCR markers for selecting drought-tolerant spring barley.

PubMed

Fiust, Anna; Rapacz, Marcin; Wójcik-Jagła, Magdalena; Tyrka, Mirosław

2015-08-01

The tolerance of spring barley (Hordeum vulgare L.) cultivars to spring drought is an important agronomic trait affecting crop yield and quality in Poland. Therefore, breeders require new molecular markers to select plants with lower spring drought susceptibility. With the advent of genomic selection technology, simple molecular tools may still be applicable to screen material for markers of the most important traits and in-depth genome scanning. In previous studies, diversity arrays technology (DArT)-based genetic maps were constructed for F2 populations of Polish fodder and malt barley elite breeding lines, and 15 and 18 quantitative trait loci (QTLs) related to spring drought tolerance were identified, respectively. In this paper, we show the results of a conversion of 30 DArT markers corresponding to 11 QTLs into simple sequence repeat (SSR) and sequence tagged site (STS) markers. Twenty-two polymorphic markers were obtained, including 13 DArT-based SSRs. Additionally, 31 SSR markers, located in close proximity to the DArT markers, were selected from the GrainGenes database and tested. Further analyses of 24 advanced breeding lines with different drought tolerances confirmed that five out of the 30 converted markers, as well as three out of the 31 additional SSR markers, were effective in marker-assisted selection for drought tolerance. The possible function of clones related to these markers in drought tolerance is discussed.

DART Core/Combustor-Noise Initial Test Results

NASA Technical Reports Server (NTRS)

Boyle, Devin K.; Henderson, Brenda S.; Hultgren, Lennart S.

2017-01-01

Contributions from the combustor to the overall propulsion noise of civilian transport aircraft are starting to become important due to turbofan design trends and advances in mitigation of other noise sources. Future propulsion systems for ultra-efficient commercial air vehicles are projected to be of increasingly higher bypass ratio from larger fans combined with much smaller cores, with ultra-clean burning fuel-flexible combustors. Unless effective noise-reduction strategies are developed, combustor noise is likely to become a prominent contributor to overall airport community noise in the future. The new NASA DGEN Aero0propulsion Research Turbofan (DART) is a cost-efficient testbed for the study of core-noise physics and mitigation. This presentation gives a brief description of the recently completed DART core combustor-noise baseline test in the NASA GRC Aero-Acoustic Propulsion Laboratory (AAPL). Acoustic data was simultaneously acquired using the AAPL overhead microphone array in the engine aft quadrant far field, a single midfield microphone, and two semi-infinite-tube unsteady pressure sensors at the core-nozzle exit. An initial assessment shows that the data is of high quality and compares well with results from a quick 2014 feasibility test. Combustor noise components of measured total-noise signatures were educed using a two-signal source-separation method an dare found to occur in the expected frequency range. The research described herein is aligned with the NASA Ultra-Efficient Commercial Transport strategic thrust and is supported by the NASA Advanced Air Vehicle Program, Advanced Air Transport Technology Project, under the Aircraft Noise Reduction Subproject.

KSC-2014-4900

NASA Image and Video Library

2014-10-31

CAPE CANAVERAL, Fla. – A researcher at NASA’s Kennedy Space Center in Florida checks a reading on the Dust Atmospheric Recovery Technology, or DART, spacecraft inside a laboratory at the Space Life Sciences Lab. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces. Photo credit: NASA/Dimitri Gerondidakis

KSC-2014-4901

NASA Image and Video Library

2014-10-31

CAPE CANAVERAL, Fla. – A researcher at NASA’s Kennedy Space Center in Florida checks a reading on the Dust Atmospheric Recovery Technology, or DART, spacecraft inside a laboratory at the Space Life Sciences Lab. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces. Photo credit: NASA/Dimitri Gerondidakis

KSC-2014-4903

NASA Image and Video Library

2014-10-31

CAPE CANAVERAL, Fla. – Researchers at NASA’s Kennedy Space Center in Florida check readings on the Dust Atmospheric Recovery Technology, or DART, spacecraft inside a laboratory at the Space Life Sciences Lab. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces. Photo credit: NASA/Dimitri Gerondidakis

KSC-2014-4902

NASA Image and Video Library

2014-10-31

CAPE CANAVERAL, Fla. – Researchers at NASA’s Kennedy Space Center in Florida check readings on the Dust Atmospheric Recovery Technology, or DART, spacecraft inside a laboratory at the Space Life Sciences Lab. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces. Photo credit: NASA/Dimitri Gerondidakis

KSC-2014-4899

NASA Image and Video Library

2014-10-31

CAPE CANAVERAL, Fla. – The Dust Atmospheric Recovery Technology, or DART, spacecraft is being assembled in a laboratory inside the Space Life Sciences Lab at NASA’s Kennedy Space Center in Florida. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces. Photo credit: NASA/Dimitri Gerondidakis

KSC-2014-4898

NASA Image and Video Library

2014-10-31

CAPE CANAVERAL, Fla. – The Dust Atmospheric Recovery Technology, or DART, spacecraft is being assembled in a laboratory inside the Space Life Sciences Lab at NASA’s Kennedy Space Center in Florida. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces. Photo credit: NASA/Dimitri Gerondidakis

Status of the Dust Accumulation and Removal Technology Experiment for the Mars 2001 Surveyor Lander

NASA Technical Reports Server (NTRS)

Jenkins, P. P.; Landis, G. L.; Krasowski, M. J.; Greer, L. C. , III; Lekki, J.; Baraona, C. R.; Scheiman, D. A.; Wilt, D. M.

1999-01-01

The Dust Accumulation and Removal Technology (DART) experiment is designed to quantify the nature of dust settling out of the Martian atmosphere. DART is part of the Mars in-situ propellant precursor (MIP) experiment which is a payload on the Mars 2001 Surveyor Lander. At the time of this writing, high fidelity development hardware has been integrated in to the MIP experiment and completed Mars environment testing. Additional information is contained in the original extended abstract.

Genome-wide DArT and SNP scan for QTL associated with resistance to stripe rust (Puccinia striiformis f. sp. tritici) in elite ICARDA wheat (Triticum aestivum L.) germplasm.

PubMed

Jighly, Abdulqader; Oyiga, Benedict C; Makdis, Farid; Nazari, Kumarse; Youssef, Omran; Tadesse, Wuletaw; Abdalla, Osman; Ogbonnaya, Francis C

2015-07-01

Identified DArT and SNP markers including a first reported QTL on 3AS, validated large effect APR on 3BS. The different genes can be used to incorporate stripe resistance in cultivated varieties. Stripe rust [yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst)] is a serious disease in wheat (Triticum aestivum). This study employed genome-wide association mapping (GWAM) to identify markers linked to stripe rust resistance genes using Diversity Arrays Technology (DArT(®)) and single-nucleotide polymorphism (SNP) Infinium 9K assays in 200 ICARDA wheat genotypes, phenotyped for seedling and adult plant resistance in two sites over two growing seasons in Syria. Only 25.8 % of the genotypes showed resistance at seedling stage while about 33 and 44 % showed moderate resistance and resistance response, respectively. Mixed-linear model adjusted for false discovery rate at p < 0.05 identified 12 DArT and 29 SNP markers on chromosome arms 3AS, 3AL, 1AL, 2AL, 2BS, 2BL, 3BS, 3BL, 5BL, 6AL, and 7DS significantly linked to Pst resistance genes. Of these, the locus on 3AS has not been previously reported to confer resistance to stripe rust in wheat. The QTL on 3AS, 3AL, 1AL, 2AL, and 2BS were effective at seedling and adult plant growth stages while those on 3BS, 3BL, 5BL, 6AL and 7DS were effective at adult plant stage. The 3BS QTL was validated in Cham-6 × Cham-8 recombinant inbred line population; composite interval analysis identified a stripe resistance QTL flanked by the DArT marker, wPt-798970, contributed by Cham-6 parent which accounted for 31.2 % of the phenotypic variation. The DArT marker "wPt-798970" lies 1.6 cM away from the 3BS QTL detected within GWAM. Epistatic interactions were also investigated; only the QTL on 1AL, 3AS and 6AL exhibited interactions with other loci. These results suggest that GWAM can be an effective approach for identifying and improving resistance to stripe rust in wheat.

The Double Asteroid Redirection Test (DART)

NASA Astrophysics Data System (ADS)

Rivkin, A.; Cheng, A. F.; Stickle, A. M.; Richardson, D. C.; Barnouin, O. S.; Thomas, C.; Fahnestock, E.

2017-12-01

The Double Asteroid Redirection Test (DART) will be the first space experiment to demonstrate asteroid impact hazard mitigation by using a kinetic impactor. DART is currently in Preliminary Design Phase ("Phase B"), and is part of the Asteroid Impact and Deflection Assessment (AIDA), a joint ESA-NASA cooperative project. The AIDA target is the near-Earth binary asteroid 65803 Didymos, an S-class system that will make a close approach to Earth in fall 2022. The DART spacecraft is designed to impact the Didymos secondary at 6 km/s and demonstrate the ability to modify its trajectory through momentum transfer. The primary goals of AIDA are (1) perform a full-scale demonstration of the spacecraft kinetic impact technique for deflection of an asteroid; (2) measure the resulting asteroid deflection, by targeting the secondary member of a binary NEO and measuring the resulting changes of the binary orbit; and (3) study hyper-velocity collision effects on an asteroid, validating models for momentum transfer in asteroid impacts. The DART impact on the Didymos secondary will change the orbital period of the binary by several minutes, which can be measured by Earth-based optical and radar observations. The baseline DART mission launches in late 2020 to impact the Didymos secondary in 2022 near the time of its close pass of Earth, which enables an array of ground- and space-based observatories to participate in gathering data. The AIDA project will provide the first measurements of momentum transfer efficiency from hyper-velocity kinetic impact at full scale on an asteroid, where the impact conditions of the projectile are known, and physical properties and internal structures of the target asteroid are characterized or constrained. The DART kinetic impact is predicted to make a crater of 6 to 17 meters diameter, depending on target physical properties, but will also release a large volume of particulate ejecta that may be directly observable from Earth or even resolvable as a coma or an ejecta tail by ground-based telescopes.

KSC-2014-4904

NASA Image and Video Library

2014-10-31

CAPE CANAVERAL, Fla. – A researcher from the University of Florida in Gainesville, checks the Dust Atmospheric Recovery Technology, or DART, spacecraft in a laboratory inside the Space Life Sciences Lab at NASA’s Kennedy Space Center in Florida. DART will characterize the dust loading and microbial diversity in the atmosphere over Florida during summer months with a special emphasis on their interactions during an African dust storm. DART will be used to collect atmospheric aerosols and suspended microbial cells over Florida and Kennedy. Results will help predict the risks of excessive microbial contamination adhering to spacecraft surfaces. Photo credit: NASA/Dimitri Gerondidakis

Root trait diversity, molecular marker diversity, and trait-marker associations in a core collection of Lupinus angustifolius.

PubMed

Chen, Yinglong; Shan, Fucheng; Nelson, Matthew N; Siddique, Kadambot Hm; Rengel, Zed

2016-06-01

Narrow-leafed lupin (Lupinus angustifolius L.) is the predominant grain legume crop in southern Australia, contributing half of the total grain legume production of Australia. Its yield in Australia is hampered by a range of subsoil constraints. The adaptation of lupin genotypes to subsoil constraints may be improved by selecting for optimal root traits from new and exotic germplasm sources. We assessed root trait diversity and genetic diversity of a core collection of narrow-leafed lupin (111 accessions) using 191 Diversity Arrays Technology (DArT) markers. The genetic relationship among accessions was determined using the admixture model in STRUCTURE. Thirty-eight root-associated traits were characterized, with 21 having coefficient of variation values >0.5. Principal coordinate analysis and cluster analysis of the DArT markers revealed broad diversity among the accessions. An ad hoc statistics calculation resulted in 10 distinct populations with significant differences among and within them (P < 0.001). The mixed linear model test in TASSEL showed a significant association between all root traits and some DArT markers, with the numbers of markers associated with an individual trait ranging from 2 to 13. The percentage of phenotypic variation explained by any one marker ranged from 6.4 to 21.8%, with 15 associations explaining >10% of phenotypic variation. The genetic variation values ranged from 0 to 7994, with 23 associations having values >240. Root traits such as deeper roots and lateral root proliferation at depth would be useful for this species for improved adaptation to drier soil conditions. This study offers opportunities for discovering useful root traits that can be used to increase the yield of Australian cultivars across variable environmental conditions. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Root trait diversity, molecular marker diversity, and trait-marker associations in a core collection of Lupinus angustifolius

PubMed Central

Chen, Yinglong; Shan, Fucheng; Nelson, Matthew N; Siddique, Kadambot HM; Rengel, Zed

2016-01-01

Narrow-leafed lupin (Lupinus angustifolius L.) is the predominant grain legume crop in southern Australia, contributing half of the total grain legume production of Australia. Its yield in Australia is hampered by a range of subsoil constraints. The adaptation of lupin genotypes to subsoil constraints may be improved by selecting for optimal root traits from new and exotic germplasm sources. We assessed root trait diversity and genetic diversity of a core collection of narrow-leafed lupin (111 accessions) using 191 Diversity Arrays Technology (DArT) markers. The genetic relationship among accessions was determined using the admixture model in STRUCTURE. Thirty-eight root-associated traits were characterized, with 21 having coefficient of variation values >0.5. Principal coordinate analysis and cluster analysis of the DArT markers revealed broad diversity among the accessions. An ad hoc statistics calculation resulted in 10 distinct populations with significant differences among and within them (P < 0.001). The mixed linear model test in TASSEL showed a significant association between all root traits and some DArT markers, with the numbers of markers associated with an individual trait ranging from 2 to 13. The percentage of phenotypic variation explained by any one marker ranged from 6.4 to 21.8%, with 15 associations explaining >10% of phenotypic variation. The genetic variation values ranged from 0 to 7994, with 23 associations having values >240. Root traits such as deeper roots and lateral root proliferation at depth would be useful for this species for improved adaptation to drier soil conditions. This study offers opportunities for discovering useful root traits that can be used to increase the yield of Australian cultivars across variable environmental conditions. PMID:27049020

Demonstration of Autonomous Rendezvous Technology (DART) Project Summary

NASA Technical Reports Server (NTRS)

Rumford, TImothy E.

2003-01-01

Since the 1960's, NASA has performed numerous rendezvous and docking missions. The common element of all US rendezvous and docking is that the spacecraft has always been piloted by astronauts. Only the Russian Space Program has developed and demonstrated an autonomous capability. The Demonstration of Autonomous Rendezvous Technology (DART) project currently funded under NASA's Space Launch Initiative (SLI) Cycle I, provides a key step in establishing an autonomous rendezvous capability for the United States. DART's objective is to demonstrate, in space, the hardware and software necessary for autonomous rendezvous. Orbital Sciences Corporation intends to integrate an Advanced Video Guidance Sensor and Autonomous Rendezvous and Proximity Operations algorithms into a Pegasus upper stage in order to demonstrate the capability to autonomously rendezvous with a target currently in orbit. The DART mission will occur in April 2004. The launch site will be Vandenburg AFB and the launch vehicle will be a Pegasus XL equipped with a Hydrazine Auxiliary Propulsion System 4th stage. All mission objectives will be completed within a 24 hour period. The paper provides a summary of mission objectives, mission overview and a discussion on the design features of the chase and target vehicles.

Genome-wide association mapping of quantitative traits in a breeding population of sugarcane.

PubMed

Racedo, Josefina; Gutiérrez, Lucía; Perera, María Francisca; Ostengo, Santiago; Pardo, Esteban Mariano; Cuenya, María Inés; Welin, Bjorn; Castagnaro, Atilio Pedro

2016-06-24

Molecular markers associated with relevant agronomic traits could significantly reduce the time and cost involved in developing new sugarcane varieties. Previous sugarcane genome-wide association analyses (GWAS) have found few molecular markers associated with relevant traits at plant-cane stage. The aim of this study was to establish an appropriate GWAS to find molecular markers associated with yield related traits consistent across harvesting seasons in a breeding population. Sugarcane clones were genotyped with DArT (Diversity Array Technology) and TRAP (Target Region Amplified Polymorphism) markers, and evaluated for cane yield (CY) and sugar content (SC) at two locations during three successive crop cycles. GWAS mapping was applied within a novel mixed-model framework accounting for population structure with Principal Component Analysis scores as random component. A total of 43 markers significantly associated with CY in plant-cane, 42 in first ratoon, and 41 in second ratoon were detected. Out of these markers, 20 were associated with CY in 2 years. Additionally, 38 significant associations for SC were detected in plant-cane, 34 in first ratoon, and 47 in second ratoon. For SC, one marker-trait association was found significant for the 3 years of the study, while twelve markers presented association for 2 years. In the multi-QTL model several markers with large allelic substitution effect were found. Sequences of four DArT markers showed high similitude and e-value with coding sequences of Sorghum bicolor, confirming the high gene microlinearity between sorghum and sugarcane. In contrast with other sugarcane GWAS studies reported earlier, the novel methodology to analyze multi-QTLs through successive crop cycles used in the present study allowed us to find several markers associated with relevant traits. Combining existing phenotypic trial data and genotypic DArT and TRAP marker characterizations within a GWAS approach including population structure as random covariates may prove to be highly successful. Moreover, sequences of DArT marker associated with the traits of interest were aligned in chromosomal regions where sorghum QTLs has previously been reported. This approach could be a valuable tool to assist the improvement of sugarcane and better supply sugarcane demand that has been projected for the upcoming decades.

A Multi-layer, Data-driven Advanced Reasoning Tool for Intelligent Data Mining and Analysis for Smart Grids

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lu, Ning; Du, Pengwei; Greitzer, Frank L.

2012-12-31

This paper presents the multi-layer, data-driven advanced reasoning tool (M-DART), a proof-of-principle decision support tool for improved power system operation. M-DART will cross-correlate and examine different data sources to assess anomalies, infer root causes, and anneal data into actionable information. By performing higher-level reasoning “triage” of diverse data sources, M-DART focuses on early detection of emerging power system events and identifies highest priority actions for the human decision maker. M-DART represents a significant advancement over today’s grid monitoring technologies that apply offline analyses to derive model-based guidelines for online real-time operations and use isolated data processing mechanisms focusing on individualmore » data domains. The development of the M-DART will bridge these gaps by reasoning about results obtained from multiple data sources that are enabled by the smart grid infrastructure. This hybrid approach integrates a knowledge base that is trained offline but tuned online to capture model-based relationships while revealing complex causal relationships among data from different domains.« less

Easier Said Than Done: Keys to Successful Implementation of the Distress Assessment and Response Tool (DART) Program.

PubMed

Li, Madeline; Macedo, Alyssa; Crawford, Sean; Bagha, Sabira; Leung, Yvonne W; Zimmermann, Camilla; Fitzgerald, Barbara; Wyatt, Martha; Stuart-McEwan, Terri; Rodin, Gary

2016-05-01

Systematic screening for distress in oncology clinics has gained increasing acceptance as a means to improve cancer care, but its implementation poses enormous challenges. We describe the development and implementation of the Distress Assessment and Response Tool (DART) program in a large urban comprehensive cancer center. DART is an electronic screening tool used to detect physical and emotional distress and practical concerns and is linked to triaged interprofessional collaborative care pathways. The implementation of DART depended on clinician education, technological innovation, transparent communication, and an evaluation framework based on principles of change management and quality improvement. There have been 364,378 DART surveys completed since 2010, with a sustained screening rate of > 70% for the past 3 years. High staff satisfaction, increased perception of teamwork, greater clinical attention to the psychosocial needs of patients, patient-clinician communication, and patient satisfaction with care were demonstrated without a resultant increase in referrals to specialized psychosocial services. DART is now a standard of care for all patients attending the cancer center and a quality performance indicator for the organization. Key factors in the success of DART implementation were the adoption of a programmatic approach, strong institutional commitment, and a primary focus on clinic-based response. We have demonstrated that large-scale routine screening for distress in a cancer center is achievable and has the potential to enhance the cancer care experience for both patients and staff. Copyright © 2016 by American Society of Clinical Oncology.

Genome-wide associations for water-soluble carbohydrate concentration and relative maturity in wheat using SNP and DArT marker arrays

USDA-ARS?s Scientific Manuscript database

Improving water-use efficiency by incorporating drought avoidance traits into new wheat varieties is an important objective for wheat breeding in water-limited environments. This study uses genome wide association studies (GWAS) to identify candidate loci for water-soluble carbohydrate accumulation,...

Real-Time and High-Fidelity Simulation Environment for Autonomous Ground Vehicle Dynamics

DTIC Science & Technology

2013-08-01

ENGINEERING AND TECHNOLOGY SYMPOSIUM (GVSETS), SET FOR AUG. 21-22, 2013 14. ABSTRACT briefing charts 15. SUBJECT TERMS 16. SECURITY CLASSIFICATION OF: 17...EDL & Aero-Flight DSENDS Airships Planetary & Terrain models SimScape Simulation framework Dshell Flex & Multibody dynamics DARTS 3D...7 DARTS Rigid/Flexible Real-Time Multibody Dynamics Engine Recipient of the NASA Software of the Year Award. Abhinandan Jain, "Robot and

Ultrafast Screening and Quantitation of Pesticides in Food and Environmental Matrices by Solid-Phase Microextraction-Transmission Mode (SPME-TM) and Direct Analysis in Real Time (DART).

PubMed

Gómez-Ríos, Germán Augusto; Gionfriddo, Emanuela; Poole, Justen; Pawliszyn, Janusz

2017-07-05

The direct interface of microextraction technologies to mass spectrometry (MS) has unquestionably revolutionized the speed and efficacy at which complex matrices are analyzed. Solid Phase Micro Extraction-Transmission Mode (SPME-TM) is a technology conceived as an effective synergy between sample preparation and ambient ionization. Succinctly, the device consists of a mesh coated with polymeric particles that extracts analytes of interest present in a given sample matrix. This coated mesh acts as a transmission-mode substrate for Direct Analysis in Real Time (DART), allowing for rapid and efficient thermal desorption/ionization of analytes previously concentrated on the coating, and dramatically lowering the limits of detection attained by sole DART analysis. In this study, we present SPME-TM as a novel tool for the ultrafast enrichment of pesticides present in food and environmental matrices and their quantitative determination by MS via DART ionization. Limits of quantitation in the subnanogram per milliliter range can be attained, while total analysis time does not exceed 2 min per sample. In addition to target information obtained via tandem MS, retrospective studies of the same sample via high-resolution mass spectrometry (HRMS) were accomplished by thermally desorbing a different segment of the microextraction device.

Evaluation of Remote Delivery of Passive Integrated Transponder (PIT) Technology to Mark Large Mammals

PubMed Central

Walter, W. David; Anderson, Charles W.; VerCauteren, Kurt C.

2012-01-01

Methods to individually mark and identify free-ranging wildlife without trapping and handling would be useful for a variety of research and management purposes. The use of Passive Integrated Transponder technology could be an efficient method for collecting data for mark-recapture analysis and other strategies for assessing characteristics about populations of various wildlife species. Passive Integrated Transponder tags (PIT) have unique numbered frequencies and have been used to successfully mark and identify mammals. We tested for successful injection of PIT and subsequent functioning of PIT into gelatin blocks using 4 variations of a prototype dart. We then selected the prototype dart that resulted in the least depth of penetration in the gelatin block to assess the ability of PIT to be successfully implanted into muscle tissue of white-tailed deer (Odocoileus virginianus) post-mortem and long-term in live, captive Rocky Mountain elk (Cervus elaphus). The prototype dart with a 12.7 mm (0.5 inch) needle length and no powder charge resulted in the shallowest mean (± SD) penetration depth into gelatin blocks of 27.0 mm (±5.6 mm) with 2.0 psi setting on the Dan-Inject CO2-pressured rifle. Eighty percent of PIT were successfully injected in the muscle mass of white-tailed deer post-mortem with a mean (± SD) penetration depth of 22.2 mm (±3.8 mm; n = 6). We injected PIT successfully into 13 live, captive elk by remote delivery at about 20 m that remained functional for 7 months. We successfully demonstrated that PIT could be remotely delivered in darts into muscle mass of large mammals and remain functional for >6 months. Although further research is warranted to fully develop the technique, remote delivery of PIT technology to large mammals is possible using prototype implant darts. PMID:22984572

In situ DART-MS as a Versatile and Rapid Dereplication Tool in Lichenology: Chemical Fingerprinting of Ophioparma ventosa.

PubMed

Le Pogam, Pierre; Le Lamer, Anne-Cécile; Legouin, Béatrice; Boustie, Joël; Rondeau, David

2016-11-01

Lichens widely occur all over the world and are known to produce unique secondary metabolites with various biological activities. To develop high-throughput screening approaches requiring little to no sample preparation to alleviate the dereplication holdup and accelerate the discovery workflow of new structures from lichens. The extracellular distribution of lichen metabolites is incentive for in situ chemical profiling of lichens using the ambient mass spectrometry DART-MS. For this purpose, the chlorolichen Ophioparma ventosa, producing an array of lichen polyphenolics that encompass the main structural classes associated to lichen chemodiversity, represented a relevant model to assess the versatility of this platform. The feasibility of this approach was first established by analysing the pure compounds known from this species prior to being extended to different solid organs of the lichen. All tested compounds could be detected in positive and negative ion modes, most often with prevalent protonated or deprotonated molecules. Only depsides underwent a significant in-source fragmentation in both ionisation modes, which should be regarded as an added value for their structural elucidation. In situ DART-MS analyses of Ophioparma ventosa provided an extensive chemical profile and noteworthy pinpointed miriquidic acid, an unusual lichen depside so far unknown within this species. At last, in situ DART-MS granted a first insight into the distribution of the metabolites within the lichen. DART-MS represents a versatile tool to the wide field of lichenology, facilitating accelerated and sharp analyses of lichens and bypassing costly and tedious procedures of solvent extraction. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

SBIR Phase II Final Report: Low cost Autonomous NMR and Multi-sensor Soil Monitoring Instrument

DOE Office of Scientific and Technical Information (OSTI.GOV)

Walsh, David O.

In this 32-month SBIR Phase 2 program, Vista Clara designed, assembled and successfully tested four new NMR instruments for soil moisture measurement and monitoring: An enhanced performance man-portable Dart NMR logging probe and control unit for rapid, mobile measurement in core holes and 2” PVC access wells; A prototype 4-level Dart NMR monitoring probe and prototype multi-sensor soil monitoring control unit for long-term unattended monitoring of soil moisture and other measurements in-situ; A non-invasive 1m x 1m Discus NMR soil moisture sensor with surface based magnet/coil array for rapid measurement of soil moisture in the top 50 cm of themore » subsurface; A non-invasive, ultra-lightweight Earth’s field surface NMR instrument for non-invasive measurement and mapping of soil moisture in the top 3 meters of the subsurface. The Phase 2 research and development achieved most, but not all of our technical objectives. The single-coil Dart in-situ sensor and control unit were fully developed, demonstrated and successfully commercialized within the Phase 2 period of performance. The multi-level version of the Dart probe was designed, assembled and demonstrated in Phase 2, but its final assembly and testing were delayed until close to the end of the Phase 2 performance period, which limited our opportunities for demonstration in field settings. Likewise, the multi-sensor version of the Dart control unit was designed and assembled, but not in time for it to be deployed for any long-term monitoring demonstrations. The prototype ultra-lightweight surface NMR instrument was developed and demonstrated, and this result will be carried forward into the development of a new flexible surface NMR instrument and commercial product in 2018.« less

A Taste of Honey.

ERIC Educational Resources Information Center

Howie, Jim; Jordan, Jeff; Millson, David

1999-01-01

Describes a dart-making project that is used as a comprehensive introduction to machining in beginning metals and technology classes. It helps the students bridge the gap between theory and practice by integrating science and technology. (JOW)

Documentation of the DART/FITT system

NASA Image and Video Library

1995-05-01

Photographic documentation of the Dextrous Anthropomorphic Robot Testbed (DART)/Full-Immersion Telepresence Testbed (FITT) system. Photos were taken in the Dexterous Robotics Lab, bldg 9N, room 113, and include: DART with upper arm cover removed (08920-1); DART head fully assembled (08922); DART head (08923,08939); DART only official view (08924-5); DART head with shell removed (08926-7); DART/FITT together with operator (08928-9); DART with tools (08930-1,08934); DART with tether hook (08932); DART with rope (08933); DART with Portable Foot Restraint (PFR) (08935); DART pulling out ORU (08936); DART with electronics panel (08937); and DART with automated ball grasp (08938).

A comparison between DART-MS and DSA-MS in the forensic analysis of writing inks.

PubMed

Drury, Nicholas; Ramotowski, Robert; Moini, Mehdi

2018-05-23

Ambient ionization mass spectrometry is gaining momentum in forensic science laboratories because of its high speed of analysis, minimal sample preparation, and information-rich results. One such application of ambient ionization methodology includes the analysis of writing inks from questioned documents where colorants of interest may not be soluble in common solvents, rendering thin layer chromatography (TLC) and separation-mass spectrometry methods such as LC/MS (-MS) impractical. Ambient ionization mass spectrometry uses a variety of ionization techniques such as penning ionization in Direct Analysis in Real Time (DART), and atmospheric pressure chemical ionization in Direct Sample Analysis (DSA), and electrospray ionization in Desorption Electrospray Ionization (DESI). In this manuscript, two of the commonly used ambient ionization techniques are compared: Perkin Elmer DSA-MS and IonSense DART in conjunction with a JEOL AccuTOF MS. Both technologies were equally successful in analyzing writing inks and produced similar spectra. DSA-MS produced less background signal likely because of its closed source configuration; however, the open source configuration of DART-MS provided more flexibility for sample positioning for optimum sensitivity and thereby allowing smaller piece of paper containing writing ink to be analyzed. Under these conditions, the minimum sample required for DART-MS was 1mm strokes of ink on paper, whereas DSA-MS required a minimum of 3mm. Moreover, both techniques showed comparable repeatability. Evaluation of the analytical figures of merit, including sensitivity, linear dynamic range, and repeatability, for DSA-MS and DART-MS analysis is provided. To the forensic context of the technique, DART-MS was applied to the analysis of United States Secret Service ink samples directly on a sampling mesh, and the results were compared with DSA-MS of the same inks on paper. Unlike analysis using separation mass spectrometry, which requires sample preparation, both DART-MS and DSA-MS successfully analyzed writing inks with minimal sample preparation. Copyright © 2018 Elsevier B.V. All rights reserved.

Why does negative CG lightning have subsequent return strokes?

NASA Astrophysics Data System (ADS)

Wilkes, R. A.; Kotovsky, D. A.; Uman, M. A.; Carvalho, F. L.; Jordan, D.

2017-12-01

It is not understood why cloud-to-ground (CG) lightning flashes lowering negative charge often produce discrete dart-leader/return-stroke sequences rather than having the first stroke drain the available cloud charge, as is almost always the case for CG lightning lowering positive charge. Triggered lightning data obtained at the International Center for Lightning Research and Testing (ICLRT) in north-central Florida have been analyzed to clarify the subsequent return-stroke process. In summers 2013 through 2016 at the ICLRT, 53% of the rocket launches did not initiate any part of a lightning flash, 13% of the rocket launches created an initial stage only (ISO) and failed to produce a following dart-leader/return-stroke sequences, and 34% of rocket launches produced an initial stage (IS) followed by return strokes. The IS of the triggered lightning consists of the upward positive leader and a following initial continuing current, both being responsible for transporting negative charge from the cloud to ground. Our ISO events may well have some commonality with the roughly 20 percent of natural CG flashes that fail to produce a dart-leader/return-stroke. We have analyzed the IS of 41 triggered lightning flashes with (19 cases) and without (22 cases) following return strokes and compared areas and heights of the flash using data collected by a Lightning Mapping Array (LMA). In our preliminary analysis, we can find no geometrical feature of the lightning channel during the IS that will predict the occurrence or lack of occurrence of following return strokes. We also have compared the triggered-lightning electrical current and charge transfer observed at the ground. We found that the average current, duration, and charge transfer during the IS for ISO events is each about half that of ISs analyzed which are followed by dart-leader/return-stroke sequences, contrary to the results presented from the GCOELD in China. Summarizing, there appear to be no differences in the channel geometry between initial stages that do or do not yield dart-leader/return-stroke sequences. In contrast, we find that particular electrical characteristics of the initial stage may indicate whether or not a dart-leader/return-stroke sequence may follow, potentially shedding light on the physical processes necessary for dart-leader initiation.

Direct analysis in real time-Mass spectrometry (DART-MS) in forensic and security applications.

PubMed

Pavlovich, Matthew J; Musselman, Brian; Hall, Adam B

2018-03-01

Over the last decade, direct analysis in real time (DART) has emerged as a viable method for fast, easy, and reliable "ambient ionization" for forensic analysis. The ability of DART to generate ions from chemicals that might be present at the scene of a criminal activity, whether they are in the gas, liquid, or solid phase, with limited sample preparation has made the technology a useful analytical tool in numerous forensic applications. This review paper summarizes many of those applications, ranging from the analysis of trace evidence to security applications, with a focus on providing the forensic scientist with a resource for developing their own applications. The most common uses for DART in forensics are in studying seized drugs, drugs of abuse and their metabolites, bulk and detonated explosives, toxic chemicals, chemical warfare agents, inks and dyes, and commercial plant and animal products that have been adulterated for economic gain. This review is meant to complement recent reviews that have described the fundamentals of the ionization mechanism and the general use of DART. We describe a wide range of forensic applications beyond the field of analyzing drugs of abuse, which dominates the literature, including common experimental and data analysis methods. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 37:171-187, 2018. © 2016 Wiley Periodicals, Inc.

Rapid process development of chromatographic process using direct analysis in real time mass spectrometry as a process analytical technology tool.

PubMed

Yan, Binjun; Chen, Teng; Xu, Zhilin; Qu, Haibin

2014-06-01

The concept of quality by design (QbD) is widely applied in the process development of pharmaceuticals. However, the additional cost and time have caused some resistance about QbD implementation. To show a possible solution, this work proposed a rapid process development method, which used direct analysis in real time mass spectrometry (DART-MS) as a process analytical technology (PAT) tool for studying the chromatographic process of Ginkgo biloba L., as an example. The breakthrough curves were fast determined by DART-MS at-line. A high correlation coefficient of 0.9520 was found between the concentrations of ginkgolide A determined by DART-MS and HPLC. Based on the PAT tool, the impacts of process parameters on the adsorption capacity were discovered rapidly, which showed a decreased adsorption capacity with the increase of the flow rate. This work has shown the feasibility and advantages of integrating PAT into QbD implementation for rapid process development. Copyright © 2014 Elsevier B.V. All rights reserved.

DART: Recent Advances in Remote Sensing Data Modeling With Atmosphere, Polarization, and Chlorophyll Fluorescence

NASA Technical Reports Server (NTRS)

Gastellu-Etchegorry, Jean-Phil; Lauret, Nicolas; Yin, Tiangang; Landier, Lucas; Kallel, Abdelaziz; Malenovsky, Zbynek; Bitar, Ahmad Al; Aval, Josselin; Benhmida, Sahar; Qi, Jianbo;

Genetic Diversity and Population Structure Analysis of European Hexaploid Bread Wheat (Triticum aestivum L.) Varieties

PubMed Central

Nielsen, Nanna Hellum; Backes, Gunter; Stougaard, Jens; Andersen, Stig Uggerhøj; Jahoor, Ahmed

2014-01-01

Progress in plant breeding is facilitated by accurate information about genetic structure and diversity. Here, Diversity Array Technology (DArT) was used to characterize a population of 94 bread wheat (Triticum aestivum L.) varieties of mainly European origin. In total, 1,849 of 7,000 tested markers were polymorphic and could be used for population structure analysis. Two major subgroups of wheat varieties, GrI and GrII, were identified using the program STRUCTURE, and confirmed by principal component analysis (PCA). These subgroups were largely separated according to origin; GrI comprised varieties from Southern and Eastern Europe, whereas GrII contained mostly modern varieties from Western and Northern Europe. A large proportion of the markers contributing most to the genetic separation of the subgroups were located on chromosome 2D near the Reduced height 8 (Rht8) locus, and PCR-based genotyping suggested that breeding for the Rht8 allele had a major impact on subgroup separation. Consistently, analysis of linkage disequilibrium (LD) suggested that different selective pressures had acted on chromosome 2D in the two subgroups. Our data provides an overview of the allele composition of bread wheat varieties anchored to DArT markers, which will facilitate targeted combination of alleles following DArT-based QTL studies. In addition, the genetic diversity and distance data combined with specific Rht8 genotypes can now be used by breeders to guide selection of crossing parents. PMID:24718292

Genetic diversity and population structure analysis of European hexaploid bread wheat (Triticum aestivum L.) varieties.

PubMed

Nielsen, Nanna Hellum; Backes, Gunter; Stougaard, Jens; Andersen, Stig Uggerhøj; Jahoor, Ahmed

2014-01-01

Progress in plant breeding is facilitated by accurate information about genetic structure and diversity. Here, Diversity Array Technology (DArT) was used to characterize a population of 94 bread wheat (Triticum aestivum L.) varieties of mainly European origin. In total, 1,849 of 7,000 tested markers were polymorphic and could be used for population structure analysis. Two major subgroups of wheat varieties, GrI and GrII, were identified using the program STRUCTURE, and confirmed by principal component analysis (PCA). These subgroups were largely separated according to origin; GrI comprised varieties from Southern and Eastern Europe, whereas GrII contained mostly modern varieties from Western and Northern Europe. A large proportion of the markers contributing most to the genetic separation of the subgroups were located on chromosome 2D near the Reduced height 8 (Rht8) locus, and PCR-based genotyping suggested that breeding for the Rht8 allele had a major impact on subgroup separation. Consistently, analysis of linkage disequilibrium (LD) suggested that different selective pressures had acted on chromosome 2D in the two subgroups. Our data provides an overview of the allele composition of bread wheat varieties anchored to DArT markers, which will facilitate targeted combination of alleles following DArT-based QTL studies. In addition, the genetic diversity and distance data combined with specific Rht8 genotypes can now be used by breeders to guide selection of crossing parents.

Genome-Wide Association Study Reveals a New QTL for Salinity Tolerance in Barley (Hordeum vulgare L.)

PubMed Central

Fan, Yun; Zhou, Gaofeng; Shabala, Sergey; Chen, Zhong-Hua; Cai, Shengguan; Li, Chengdao; Zhou, Meixue

2016-01-01

Salinity stress is one of the most severe abiotic stresses that affect agricultural production. Genome wide association study (GWAS) has been widely used to detect genetic variations in extensive natural accessions with more recombination and higher resolution. In this study, 206 barley accessions collected worldwide were genotyped with 408 Diversity Arrays Technology (DArT) markers and evaluated for salinity stress tolerance using salinity tolerance score – a reliable trait developed in our previous work. GWAS for salinity tolerance had been conducted through a general linkage model and a mixed linkage model based on population structure and kinship. A total of 24 significant marker-trait associations were identified. A QTL on 4H with the nearest marker of bPb-9668 was consistently detected in all different methods. This QTL has not been reported before and is worth to be further confirmed with bi-parental populations. PMID:27446173

Trajectory options for the DART mission

NASA Astrophysics Data System (ADS)

Atchison, Justin A.; Ozimek, Martin T.; Kantsiper, Brian L.; Cheng, Andrew F.

2016-06-01

This study presents interplanetary trajectory options for the Double Asteroid Redirection Test (DART) spacecraft to reach the near Earth object, Didymos binary system, during its 2022 Earth conjunction. DART represents a component of a joint NASA-ESA mission to study near Earth object kinetic impact deflection. The DART trajectory must satisfy mission objectives for arrival timing, geometry, and lighting while minimizing launch vehicle and spacecraft propellant requirements. Chemical propulsion trajectories are feasible from two candidate launch windows in late 2020 and 2021. The 2020 trajectories are highly perturbed by Earth's orbit, requiring post-launch deep space maneuvers to retarget the Didymos system. Within these windows, opportunities exist for flybys of additional near Earth objects: Orpheus in 2021 or 2007 YJ in 2022. A second impact attempt, in the event that the first impact is unsuccessful, can be added at the expense of a shorter launch window and increased (∼3x) spacecraft ΔV . However, the second impact arrival geometry has poor lighting, high Earth ranges, and would require additional degrees of freedom for solar panel and/or antenna gimbals. A low-thrust spacecraft configuration increases the trajectory flexibility. A solar electric propulsion spacecraft could be affordably launched as a secondary spacecraft in an Earth orbit and spiral out to target the requisite interplanetary departure condition. A sample solar electric trajectory was constructed from an Earth geostationary transfer using a representative 1.5 kW thruster. The trajectory requires 9 months to depart Earth's sphere of influence, after which its interplanetary trajectory includes a flyby of Orpheus and a second Didymos impact attempt. The solar electric spacecraft implementation would impose additional bus design constraints, including large solar arrays that could pose challenges for terminal guidance. On the basis of this study, there are many feasible options for DART to meet its mission design objectives and enable this unique kinetic impact experiment.

Characterization of the Drosophila protein arginine methyltransferases DART1 and DART4.

PubMed

Boulanger, Marie-Chloé; Miranda, Tina Branscombe; Clarke, Steven; Di Fruscio, Marco; Suter, Beat; Lasko, Paul; Richard, Stéphane

2004-04-15

The role of arginine methylation in Drosophila melanogaster is unknown. We identified a family of nine PRMTs (protein arginine methyltransferases) by sequence homology with mammalian arginine methyltransferases, which we have named DART1 to DART9 ( Drosophila arginine methyltransferases 1-9). In keeping with the mammalian PRMT nomenclature, DART1, DART4, DART5 and DART7 are the putative homologues of PRMT1, PRMT4, PRMT5 and PRMT7. Other DART family members have a closer resemblance to PRMT1, but do not have identifiable homologues. All nine genes are expressed in Drosophila at various developmental stages. DART1 and DART4 have arginine methyltransferase activity towards substrates, including histones and RNA-binding proteins. Amino acid analysis of the methylated arginine residues confirmed that both DART1 and DART4 catalyse the formation of asymmetrical dimethylated arginine residues and they are type I arginine methyltransferases. The presence of PRMTs in D. melanogaster suggest that flies are a suitable genetic system to study arginine methylation.

Characterization of the Drosophila protein arginine methyltransferases DART1 and DART4.

PubMed Central

Boulanger, Marie-Chloé; Miranda, Tina Branscombe; Clarke, Steven; Di Fruscio, Marco; Suter, Beat; Lasko, Paul; Richard, Stéphane

2004-01-01

The role of arginine methylation in Drosophila melanogaster is unknown. We identified a family of nine PRMTs (protein arginine methyltransferases) by sequence homology with mammalian arginine methyltransferases, which we have named DART1 to DART9 ( Drosophila arginine methyltransferases 1-9). In keeping with the mammalian PRMT nomenclature, DART1, DART4, DART5 and DART7 are the putative homologues of PRMT1, PRMT4, PRMT5 and PRMT7. Other DART family members have a closer resemblance to PRMT1, but do not have identifiable homologues. All nine genes are expressed in Drosophila at various developmental stages. DART1 and DART4 have arginine methyltransferase activity towards substrates, including histones and RNA-binding proteins. Amino acid analysis of the methylated arginine residues confirmed that both DART1 and DART4 catalyse the formation of asymmetrical dimethylated arginine residues and they are type I arginine methyltransferases. The presence of PRMTs in D. melanogaster suggest that flies are a suitable genetic system to study arginine methylation. PMID:14705965

SpecPad: device-independent NMR data visualization and processing based on the novel DART programming language and Html5 Web technology.

PubMed

Guigas, Bruno

2017-09-01

SpecPad is a new device-independent software program for the visualization and processing of one-dimensional and two-dimensional nuclear magnetic resonance (NMR) time domain (FID) and frequency domain (spectrum) data. It is the result of a project to investigate whether the novel programming language DART, in combination with Html5 Web technology, forms a suitable base to write an NMR data evaluation software which runs on modern computing devices such as Android, iOS, and Windows tablets as well as on Windows, Linux, and Mac OS X desktop PCs and notebooks. Another topic of interest is whether this technique also effectively supports the required sophisticated graphical and computational algorithms. SpecPad is device-independent because DART's compiled executable code is JavaScript and can, therefore, be run by the browsers of PCs and tablets. Because of Html5 browser cache technology, SpecPad may be operated off-line. Network access is only required during data import or export, e.g. via a Cloud service, or for software updates. A professional and easy to use graphical user interface consistent across all hardware platforms supports touch screen features on mobile devices for zooming and panning and for NMR-related interactive operations such as phasing, integration, peak picking, or atom assignment. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

Development and Application of a Low Frequency Near-Field Interferometric-TOA 3D Lightning Mapping Array

NASA Astrophysics Data System (ADS)

Lyu, F.; Cummer, S. A.; Weinert, J. L.; McTague, L. E.; Solanki, R.; Barrett, J.

2014-12-01

Lightning processes radiated extremely wideband electromagnetic signals. Lightning images mapped by VHF interferometry and VHF time of arrival lightning mapping arrays enable us to understand the lightning in-cloud detail development during the extent of flash that can not always be captured by cameras because of the shield of cloud. Lightning processes radiate electromagnetically over an extremely wide bandwidth, offering the possibility of multispectral lightning radio imaging. Low frequency signals are often used for lightning detection, but usually only for ground point location or thunderstorm tracking. Some recent results have demonstrated lightning LF 3D mapping of discrete lightning pulses, but imaging of continuous LF emissions have not been shown. In this work, we report a GPS-synchronized LF near field interferometric-TOA 3D lightning mapping array applied to image the development of lightning flashes on second time scale. Cross-correlation, as used in broadband interferometry, is applied in our system to find windowed arrival time differences with sub-microsecond time resolution. However, because the sources are in the near field of the array, time of arrival processing is used to find the source locations with a typical precision of 100 meters. We show that this system images the complete lightning flash structure with thousands of LF sources for extensive flashes. Importantly, this system is able to map both continuous emissions like dart leaders, and bursty or discrete emissions. Lightning stepped leader and dart leader propagation speeds are estimated to 0.56-2.5x105 m/s and 0.8-2.0x106 m/s respectively, which are consistent with previous reports. In many aspects our LF images are remarkably similar to VHF lightning mapping array images, despite the 1000 times difference in frequency, which may suggest some special links between the LF and VHF emission during lightning processes.

Transposition behavior of nonautonomous a hAT superfamily transposon nDart in rice (Oryza sativa L.).

PubMed

Fujino, Kenji; Sekiguchi, Hiroshi

2011-08-01

Transposable elements (TEs) have a significant impact on the evolution of gene function and genome structures. An endogenous nonautonomous transposable element nDart was discovered in an albino mutant that had an insertion in the Mg-protoporphyrin IX methyltransferase gene in rice. In this study, we elucidated the transposition behavior of nDart, the frequency of nDart transposition and characterized the footprint of nDart. Novel independent nDart insertions in backcrossed progenies were detected by DNA blotting analysis. In addition, germinal excision of nDart occurred at very low frequency compared with that of somatic excision, 0-13.3%, in the nDart1-4(3-2) and nDart1-A loci by a locus-specific PCR strategy. A total of 253 clones from somatic excision at five nDart loci in 10 varieties were determined. nDart rarely caused deletions beyond target site duplication (TSD). The footprint of nDart contained few transversions of nucleotides flanking to both sides of the TSD. The predominant footprint of nDart was an 8-bp addition. Precise excision of nDart was detected at a rate of only 2.2%, which occurred at two loci among the five loci examined. Furthermore, the results in this study revealed that a highly conserved mechanism of transposition is involved between maize Ac/Ds and rice Dart/nDart, which are two-component transposon systems of the hAT superfamily transposons in plant species.

The Dust at Altitude Recovery Technology (DART) System was Developed to Recover Plant, Human, and Animal Pathogens in Asian and African Dust Storms over North America

NASA Astrophysics Data System (ADS)

Schuerger, A. C.; Tench, B.; Nehr, A.; Emmons, T.; Valbuena, F.; Palaia, J.; Sugars, C.

2014-12-01

Dust emanates year-round from Africa and Asia and impacts air quality in North America. Asian dust plumes deliver up to 64 million tonnes of dust over the NW of the USA, and African dust storms deliver over 50 million tonnes of dust over Florida each year. Several recent studies have demonstrated that human and plant pathogens from Asian [1] African [2] aerosols can be transported to N. America in naturally occurring dust storms. What is unknown is whether these 'presumptive pathogens' impact human, plant, or animal health in the USA. In order to initiate a long-term monitoring program of pathogens in Asian and African dust plumes, we have developed a dust collection system called DART (Dust at Altitude Recovery Technology) (figure). The DART dust sampler can be mounted on a F104 Starfighter jet (figure) and a T6 Texan propeller driven airplane (not shown), and was test flown over FL in Dec. 2013 on the F104 and on the T6 in the summer of 2014. The DART system utilizes a high-volume pump to pass air through 6 separate filtration units where both aerosols and microbial cells are captured. The filtration systems exhibit flow rates from 25-142 L/min depending on the pore size and brand of filters used. Flow rates are directly correlated to increased air speed, and are inversely correlated to increased altitude. Filtration units can be turned on and off individually as required for specific science flight objectives. The DART dust sampler has performed nominally up to 7600 m, 0.92 Mach, and 3.5 +G's. During initial test flights in Dec. 2013, 5 of 8 genera of fungi recovered from the lower atmosphere over FL contained plant pathogens including species in the genera: Acremonium, Aspergillus, Cladosporium, Curvularia, and Fusarium. Numbers of recovered fungi, but not bacteria, increased significantly when 5 or 10 µm filters were used in the DART system compared to filter pore sizes ≤ 1.2 µm. Future sampling programs for both Asian and African dust events will be discussed. References: [1] Smith, D. J., et al., 2012. Microbial Ecology 64,973-985. [2] Griffin, D. W. 2007, Clinical Microbiology Reviews 20, 459-477.

Genome-wide association study for crown rust (Puccinia coronata f. sp. avenae) and powdery mildew (Blumeria graminis f. sp. avenae) resistance in an oat (Avena sativa) collection of commercial varieties and landraces.

PubMed

Montilla-Bascón, Gracia; Rispail, Nicolas; Sánchez-Martín, Javier; Rubiales, Diego; Mur, Luis A J; Langdon, Tim; Howarth, Catherine J; Prats, Elena

2015-01-01

Diseases caused by crown rust (Puccinia coronata f. sp. avenae) and powdery mildew (Blumeria graminis f. sp. avenae) are among the most important constraints for the oat crop. Breeding for resistance is one of the most effective, economical, and environmentally friendly means to control these diseases. The purpose of this work was to identify elite alleles for rust and powdery mildew resistance in oat by association mapping to aid selection of resistant plants. To this aim, 177 oat accessions including white and red oat cultivars and landraces were evaluated for disease resistance and further genotyped with 31 simple sequence repeat and 15,000 Diversity Arrays Technology (DArT) markers to reveal association with disease resistance traits. After data curation, 1712 polymorphic markers were considered for association analysis. Principal component analysis and a Bayesian clustering approach were applied to infer population structure. Five different general and mixed linear models accounting for population structure and/or kinship corrections and two different statistical tests were carried out to reduce false positive. Five markers, two of them highly significant in all models tested were associated with rust resistance. No strong association between any marker and powdery mildew resistance at the seedling stage was identified. However, one DArT sequence, oPt-5014, was strongly associated with powdery mildew resistance in adult plants. Overall, the markers showing the strongest association in this study provide ideal candidates for further studies and future inclusion in strategies of marker-assisted selection.

Direct analysis in real time mass spectrometry, a process analytical technology tool for real-time process monitoring in botanical drug manufacturing.

PubMed

Wang, Lu; Zeng, Shanshan; Chen, Teng; Qu, Haibin

2014-03-01

A promising process analytical technology (PAT) tool has been introduced for batch processes monitoring. Direct analysis in real time mass spectrometry (DART-MS), a means of rapid fingerprint analysis, was applied to a percolation process with multi-constituent substances for an anti-cancer botanical preparation. Fifteen batches were carried out, including ten normal operations and five abnormal batches with artificial variations. The obtained multivariate data were analyzed by a multi-way partial least squares (MPLS) model. Control trajectories were derived from eight normal batches, and the qualification was tested by R(2) and Q(2). Accuracy and diagnosis capability of the batch model were then validated by the remaining batches. Assisted with high performance liquid chromatography (HPLC) determination, process faults were explained by corresponding variable contributions. Furthermore, a batch level model was developed to compare and assess the model performance. The present study has demonstrated that DART-MS is very promising in process monitoring in botanical manufacturing. Compared with general PAT tools, DART-MS offers a particular account on effective compositions and can be potentially used to improve batch quality and process consistency of samples in complex matrices. Copyright © 2014 Elsevier B.V. All rights reserved.

Direct Analysis in Real Time Mass Spectrometry for Characterization of Large Saccharides.

PubMed

Ma, Huiying; Jiang, Qing; Dai, Diya; Li, Hongli; Bi, Wentao; Da Yong Chen, David

2018-03-06

Polysaccharide characterization posts the most difficult challenge to available analytical technologies compared to other types of biomolecules. Plant polysaccharides are reported to have numerous medicinal values, but their effect can be different based on the types of plants, and even regions of productions and conditions of cultivation. However, the molecular basis of the differences of these polysaccharides is largely unknown. In this study, direct analysis in real time mass spectrometry (DART-MS) was used to generate polysaccharide fingerprints. Large saccharides can break down into characteristic small fragments in the DART source via pyrolysis, and the products are then detected by high resolution MS. Temperature was shown to be a crucial parameter for the decomposition of large polysaccharide. The general behavior of carbohydrates in DART-MS was also studied through the investigation of a number of mono- and oligosaccharide standards. The chemical formula and putative ionic forms of the fragments were proposed based on accurate mass with less than 10 ppm mass errors. Multivariate data analysis shows the clear differentiation of different plant species. Intensities of marker ions compared among samples also showed obvious differences. The combination of DART-MS analysis and mechanochemical extraction method used in this work demonstrates a simple, fast, and high throughput analytical protocol for the efficient evaluation of molecular features in plant polysaccharides.

Lightning Mapping With an Array of Fast Antennas

NASA Astrophysics Data System (ADS)

Wu, Ting; Wang, Daohong; Takagi, Nobuyuki

2018-04-01

Fast Antenna Lightning Mapping Array (FALMA), a low-frequency lightning mapping system comprising an array of fast antennas, was developed and established in Gifu, Japan, during the summer of 2017. Location results of two hybrid flashes and a cloud-to-ground flash comprising 11 return strokes (RSs) are described in detail in this paper. Results show that concurrent branches of stepped leaders can be readily resolved, and K changes and dart leaders with speeds up to 2.4 × 107 m/s are also well imaged. These results demonstrate that FALMA can reconstruct three-dimensional structures of lightning flashes with great details. Location accuracy of FALMA is estimated by comparing the located striking points of successive RSs in cloud-to-ground flashes. Results show that distances between successive RSs are mainly below 25 m, indicating exceptionally high location accuracy of FALMA.

Darters: Who Needs Vision?

ERIC Educational Resources Information Center

Mastro, James V.; Johnston, Patrick K.; Coggins, Porter E., III

2017-01-01

This article presents a very brief history of recreational and competitive darts, presents relevant neurological systems pertinent to throwing darts, discusses dart equipment, and talks about techniques of throwing darts as a recreational and competitive sport. In particular, the performance aspect of darts for individuals with visual impairment…

Classroom sound can be used to classify teaching practices in college science courses.

PubMed

Owens, Melinda T; Seidel, Shannon B; Wong, Mike; Bejines, Travis E; Lietz, Susanne; Perez, Joseph R; Sit, Shangheng; Subedar, Zahur-Saleh; Acker, Gigi N; Akana, Susan F; Balukjian, Brad; Benton, Hilary P; Blair, J R; Boaz, Segal M; Boyer, Katharyn E; Bram, Jason B; Burrus, Laura W; Byrd, Dana T; Caporale, Natalia; Carpenter, Edward J; Chan, Yee-Hung Mark; Chen, Lily; Chovnick, Amy; Chu, Diana S; Clarkson, Bryan K; Cooper, Sara E; Creech, Catherine; Crow, Karen D; de la Torre, José R; Denetclaw, Wilfred F; Duncan, Kathleen E; Edwards, Amy S; Erickson, Karen L; Fuse, Megumi; Gorga, Joseph J; Govindan, Brinda; Green, L Jeanette; Hankamp, Paul Z; Harris, Holly E; He, Zheng-Hui; Ingalls, Stephen; Ingmire, Peter D; Jacobs, J Rebecca; Kamakea, Mark; Kimpo, Rhea R; Knight, Jonathan D; Krause, Sara K; Krueger, Lori E; Light, Terrye L; Lund, Lance; Márquez-Magaña, Leticia M; McCarthy, Briana K; McPheron, Linda J; Miller-Sims, Vanessa C; Moffatt, Christopher A; Muick, Pamela C; Nagami, Paul H; Nusse, Gloria L; Okimura, Kristine M; Pasion, Sally G; Patterson, Robert; Pennings, Pleuni S; Riggs, Blake; Romeo, Joseph; Roy, Scott W; Russo-Tait, Tatiane; Schultheis, Lisa M; Sengupta, Lakshmikanta; Small, Rachel; Spicer, Greg S; Stillman, Jonathon H; Swei, Andrea; Wade, Jennifer M; Waters, Steven B; Weinstein, Steven L; Willsie, Julia K; Wright, Diana W; Harrison, Colin D; Kelley, Loretta A; Trujillo, Gloriana; Domingo, Carmen R; Schinske, Jeffrey N; Tanner, Kimberly D

2017-03-21

Active-learning pedagogies have been repeatedly demonstrated to produce superior learning gains with large effect sizes compared with lecture-based pedagogies. Shifting large numbers of college science, technology, engineering, and mathematics (STEM) faculty to include any active learning in their teaching may retain and more effectively educate far more students than having a few faculty completely transform their teaching, but the extent to which STEM faculty are changing their teaching methods is unclear. Here, we describe the development and application of the machine-learning-derived algorithm Decibel Analysis for Research in Teaching (DART), which can analyze thousands of hours of STEM course audio recordings quickly, with minimal costs, and without need for human observers. DART analyzes the volume and variance of classroom recordings to predict the quantity of time spent on single voice (e.g., lecture), multiple voice (e.g., pair discussion), and no voice (e.g., clicker question thinking) activities. Applying DART to 1,486 recordings of class sessions from 67 courses, a total of 1,720 h of audio, revealed varied patterns of lecture (single voice) and nonlecture activity (multiple and no voice) use. We also found that there was significantly more use of multiple and no voice strategies in courses for STEM majors compared with courses for non-STEM majors, indicating that DART can be used to compare teaching strategies in different types of courses. Therefore, DART has the potential to systematically inventory the presence of active learning with ∼90% accuracy across thousands of courses in diverse settings with minimal effort.

Rapid screening of abused drugs by direct analysis in real time (DART) coupled to time-of-flight mass spectrometry (TOF-MS) combined with ion mobility spectrometry (IMS).

PubMed

Lian, Ru; Wu, Zhongping; Lv, Xiaobao; Rao, Yulan; Li, Haiyang; Li, Jinghua; Wang, Rong; Ni, Chunfang; Zhang, Yurong

2017-10-01

Increasing in cases involving drugs of abuse leads to heavy burden for law enforcement agencies, exacerbating demand for rapid screening technique. In this study, atmospheric pressure ionization technologies including direct analysis in real time (DART) ion source coupled to a time-of-flight mass spectrometer (DART-TOF-MS)as well asdopant-assisted positive photoionization ion mobility spectrometry (DAPP-IMS) without radioactivity were utilized together as the powerful analytical tool for the rapid screening and identification of 53 abused drugs.The limits of detection (LOD) were 0.05-2μg/mL when using DART-TOF-MS and 0.02-2μg when using DAPP-IMS which could satisfy the actual requirement in forensic science laboratory. The advantages of this method included fast response, high-throughput potential, high specificity, and minimal sample preparation. A screening library of reduced mobility (K 0 ), accurate mass of informative precursor ion ([M+H] + ) and fragment ions was established respectively by employing a bench-top DAPP-IMS and TOF-MS in-source collision induced dissociation (CID) mode. Then the standardized screening procedure was developed with criteria for the confirmation of positive result. A total of 50 seized drug samples provided by local forensic laboratory we reanalyzed to testify the utility of the method. This study suggests that a method combing DART-TOF-MS and DAPP-IMS is promising for the rapid screening and identification of abused drugs with minimal sample preparation and absence of chromatography. Copyright © 2017 Elsevier B.V. All rights reserved.

Classroom sound can be used to classify teaching practices in college science courses

PubMed Central

Seidel, Shannon B.; Wong, Mike; Bejines, Travis E.; Lietz, Susanne; Perez, Joseph R.; Sit, Shangheng; Subedar, Zahur-Saleh; Acker, Gigi N.; Akana, Susan F.; Balukjian, Brad; Benton, Hilary P.; Blair, J. R.; Boaz, Segal M.; Boyer, Katharyn E.; Bram, Jason B.; Burrus, Laura W.; Byrd, Dana T.; Caporale, Natalia; Carpenter, Edward J.; Chan, Yee-Hung Mark; Chen, Lily; Chovnick, Amy; Chu, Diana S.; Clarkson, Bryan K.; Cooper, Sara E.; Creech, Catherine; Crow, Karen D.; de la Torre, José R.; Denetclaw, Wilfred F.; Duncan, Kathleen E.; Edwards, Amy S.; Erickson, Karen L.; Fuse, Megumi; Gorga, Joseph J.; Govindan, Brinda; Green, L. Jeanette; Hankamp, Paul Z.; Harris, Holly E.; He, Zheng-Hui; Ingalls, Stephen; Ingmire, Peter D.; Jacobs, J. Rebecca; Kamakea, Mark; Kimpo, Rhea R.; Knight, Jonathan D.; Krause, Sara K.; Krueger, Lori E.; Light, Terrye L.; Lund, Lance; Márquez-Magaña, Leticia M.; McCarthy, Briana K.; McPheron, Linda J.; Miller-Sims, Vanessa C.; Moffatt, Christopher A.; Muick, Pamela C.; Nagami, Paul H.; Nusse, Gloria L.; Okimura, Kristine M.; Pasion, Sally G.; Patterson, Robert; Riggs, Blake; Romeo, Joseph; Roy, Scott W.; Russo-Tait, Tatiane; Schultheis, Lisa M.; Sengupta, Lakshmikanta; Small, Rachel; Spicer, Greg S.; Stillman, Jonathon H.; Swei, Andrea; Wade, Jennifer M.; Waters, Steven B.; Weinstein, Steven L.; Willsie, Julia K.; Wright, Diana W.; Harrison, Colin D.; Kelley, Loretta A.; Trujillo, Gloriana; Domingo, Carmen R.; Schinske, Jeffrey N.; Tanner, Kimberly D.

2017-01-01

Active-learning pedagogies have been repeatedly demonstrated to produce superior learning gains with large effect sizes compared with lecture-based pedagogies. Shifting large numbers of college science, technology, engineering, and mathematics (STEM) faculty to include any active learning in their teaching may retain and more effectively educate far more students than having a few faculty completely transform their teaching, but the extent to which STEM faculty are changing their teaching methods is unclear. Here, we describe the development and application of the machine-learning–derived algorithm Decibel Analysis for Research in Teaching (DART), which can analyze thousands of hours of STEM course audio recordings quickly, with minimal costs, and without need for human observers. DART analyzes the volume and variance of classroom recordings to predict the quantity of time spent on single voice (e.g., lecture), multiple voice (e.g., pair discussion), and no voice (e.g., clicker question thinking) activities. Applying DART to 1,486 recordings of class sessions from 67 courses, a total of 1,720 h of audio, revealed varied patterns of lecture (single voice) and nonlecture activity (multiple and no voice) use. We also found that there was significantly more use of multiple and no voice strategies in courses for STEM majors compared with courses for non-STEM majors, indicating that DART can be used to compare teaching strategies in different types of courses. Therefore, DART has the potential to systematically inventory the presence of active learning with ∼90% accuracy across thousands of courses in diverse settings with minimal effort. PMID:28265087

DART: New Research Using Ensemble Data Assimilation in Geophysical Models

NASA Astrophysics Data System (ADS)

Hoar, T. J.; Raeder, K.

2015-12-01

The Data Assimilation Research Testbed (DART) is a community facilityfor ensemble data assimilation developed and supported by the NationalCenter for Atmospheric Research. DART provides a comprehensive suite of software, documentation, and tutorials that can be used for ensemble data assimilation research, operations, and education. Scientists and software engineers at NCAR are available to support DART users who want to use existing DART products or develop their own applications. Current DART users range from university professors teaching data assimilation, to individual graduate students working with simple models, through national laboratories doing operational prediction with large state-of-the-art models. DART runs efficiently on many computational platforms ranging from laptops through thousands of cores on the newest supercomputers.This poster focuses on several recent research activities using DART with geophysical models.Using CAM/DART to understand whether OCO-2 Total Precipitable Water observations can be useful in numerical weather prediction.Impacts of the synergistic use of Infra-red CO retrievals (MOPITT, IASI) in CAM-CHEM/DART assimilations.Assimilation and Analysis of Observations of Amazonian Biomass Burning Emissions by MOPITT (aerosol optical depth), MODIS (carbon monoxide) and MISR (plume height).Long term evaluation of the chemical response of MOPITT-CO assimilation in CAM-CHEM/DART OSSEs for satellite planning and emission inversion capabilities.Improved forward observation operators for land models that have multiple land use/land cover segments in a single grid cell,Simulating mesoscale convective systems (MCSs) using a variable resolution, unstructured grid in the Model for Prediction Across Scales (MPAS) and DART.The mesoscale WRF+DART system generated an ensemble of year-long, real-time initializations of a convection allowing model over the United States.Constraining WACCM with observations in the tropical band (30S-30N) using DART also constrains the polar stratosphere during the same winter. Assimilation of MOPITT carbon monoxide Compact Phase Space Retrievals (CPSR) in WRF-Chem/DART.Future work:DART interface to the CICE (CESM) sea ice model.Fully coupled assimilations in CESM.

The function of dart behavior in the paper wasp, Polistes fuscatus.

PubMed

Sumana, A; Starks, Philip T

2004-05-01

Dominance behavior in Polistes wasps is a composite trait consisting of various discrete behaviors such as darts, lunges, bites, and mounts. The majority of these behaviors are considered "aggressive", and these aggressive behaviors are considered to form a continuum from mild (e.g., darts) to severe (e.g., falling fights). In this paper we focus on darts, the most common of the dominance behaviors, and investigate their function in un-manipulated post-emergent colonies of the primitively eusocial wasp P. fuscatus. Here we show that darts are correlated with the more severe dominance behaviors, and that dominance ranks do not change with the addition or exclusion of darts. We find no correlation, however, between receiving darts and receiving more severe dominance behaviors. This result suggests that darts are not indicative of aggressive reinforcement of dominance, but rather may serve a different function. Our data suggest that the function of darts is to regulate activity on nests. Both foundresses and workers dart inactive workers significantly more often than by chance, and workers respond to a foundress's (but not a worker's) dart by becoming less inactive. We also found that active workers who receive a dart from either a foundress or worker respond mostly by switching from one activity to another. Thus, our data suggest that darts are not aggressive behaviors, that foundresses use this signal to initiate activity, and that foundresses and workers both use the signal to regulate worker activity.

Identification of Direct Protein Targets of Small Molecules

PubMed Central

2010-01-01

Small-molecule target identification is a vital and daunting task for the chemical biology community as well as for researchers interested in applying the power of chemical genetics to impact biology and medicine. To overcome this “target ID” bottleneck, new technologies are being developed that analyze protein–drug interactions, such as drug affinity responsive target stability (DARTS), which aims to discover the direct binding targets (and off targets) of small molecules on a proteome scale without requiring chemical modification of the compound. Here, we review the DARTS method, discuss why it works, and provide new perspectives for future development in this area. PMID:21077692

Comparison Between Linear and Non-parametric Regression Models for Genome-Enabled Prediction in Wheat

PubMed Central

Pérez-Rodríguez, Paulino; Gianola, Daniel; González-Camacho, Juan Manuel; Crossa, José; Manès, Yann; Dreisigacker, Susanne

2012-01-01

In genome-enabled prediction, parametric, semi-parametric, and non-parametric regression models have been used. This study assessed the predictive ability of linear and non-linear models using dense molecular markers. The linear models were linear on marker effects and included the Bayesian LASSO, Bayesian ridge regression, Bayes A, and Bayes B. The non-linear models (this refers to non-linearity on markers) were reproducing kernel Hilbert space (RKHS) regression, Bayesian regularized neural networks (BRNN), and radial basis function neural networks (RBFNN). These statistical models were compared using 306 elite wheat lines from CIMMYT genotyped with 1717 diversity array technology (DArT) markers and two traits, days to heading (DTH) and grain yield (GY), measured in each of 12 environments. It was found that the three non-linear models had better overall prediction accuracy than the linear regression specification. Results showed a consistent superiority of RKHS and RBFNN over the Bayesian LASSO, Bayesian ridge regression, Bayes A, and Bayes B models. PMID:23275882

Comparison between linear and non-parametric regression models for genome-enabled prediction in wheat.

PubMed

Pérez-Rodríguez, Paulino; Gianola, Daniel; González-Camacho, Juan Manuel; Crossa, José; Manès, Yann; Dreisigacker, Susanne

2012-12-01

In genome-enabled prediction, parametric, semi-parametric, and non-parametric regression models have been used. This study assessed the predictive ability of linear and non-linear models using dense molecular markers. The linear models were linear on marker effects and included the Bayesian LASSO, Bayesian ridge regression, Bayes A, and Bayes B. The non-linear models (this refers to non-linearity on markers) were reproducing kernel Hilbert space (RKHS) regression, Bayesian regularized neural networks (BRNN), and radial basis function neural networks (RBFNN). These statistical models were compared using 306 elite wheat lines from CIMMYT genotyped with 1717 diversity array technology (DArT) markers and two traits, days to heading (DTH) and grain yield (GY), measured in each of 12 environments. It was found that the three non-linear models had better overall prediction accuracy than the linear regression specification. Results showed a consistent superiority of RKHS and RBFNN over the Bayesian LASSO, Bayesian ridge regression, Bayes A, and Bayes B models.

Dopant-assisted direct analysis in real time mass spectrometry with argon gas.

PubMed

Cody, Robert B; Dane, A John

2016-05-30

Dopants used with Atmospheric Pressure Photoionization (APPI) were examined with the Direct Analysis in Real Time (DART ® ) ion source operated with argon gas. Charge-exchange and proton transfer reactions were observed by adding toluene, anisole, chlorobenzene and acetone to the DART gas stream, complementing the information obtained by helium DART. Mass spectra were acquired with a time-of-flight mass spectrometer equipped with a DART ion source operated with argon gas. A syringe pump was used to introduce dopants directly into the DART gas stream through deactivated fused-silica capillary tubing. Samples including polycyclic aromatic hydrocarbons (PAHs), diesel fuel, trinitrotoluene and cannabinoids were deposited onto the sealed end of melting tube, allowed to dry, and the tube was then suspended in the dopant-enhanced DART gas stream. PAHs could be detected as molecular ions at concentrations in the low parts-per-billion range by using a solution of 0.5% anisole in toluene as a dopant. Argon DART analysis of a diesel fuel sample with the same dopant mixture showed a simpler mass spectrum than obtained by using helium DART. The argon DART mass spectrum was dominated by molecular ions for aromatic compounds, whereas the helium DART mass spectrum showed both molecular ions and protonated molecules. In contrast O 2 - attachment DART showed saturated hydrocarbons and oxygen-containing species. Mass spectra for trinitrotoluene with argon DART in negative-ion mode showed a prominent [M - H] - peak, whereas conventional helium DART showed both M - and [M - H] - . Lastly, in analogy to a report in the literature using APPI, positive ions produced by argon DART ionization for delta-9-tetrahydrocannabinol (THC) and cannabidiol showed distinctive product-ion mass spectra. Dopant-assisted argon DART operates by a mechanism that is analogous to those proposed for dopant-assisted atmospheric-pressure photoionization. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

DaRT: A CALL System to Help Students Practice and Develop Reasoning Skills in Choosing English Articles.

ERIC Educational Resources Information Center

Yoshii, Rika; Milne, Alastair

1998-01-01

Describes DaRT, a computer assisted language-learning system for helping English-as-a-Second-Language students master English articles. DaRT uses a diagrammatic reasoning tool to present communicative contexts for exercises in choosing appropriate articles. This paper describes the development of DaRT and DaRT's system components and concludes…

A low-frequency near-field interferometric-TOA 3-D Lightning Mapping Array

NASA Astrophysics Data System (ADS)

Lyu, Fanchao; Cummer, Steven A.; Solanki, Rahulkumar; Weinert, Joel; McTague, Lindsay; Katko, Alex; Barrett, John; Zigoneanu, Lucian; Xie, Yangbo; Wang, Wenqi

2014-11-01

We report on the development of an easily deployable LF near-field interferometric-time of arrival (TOA) 3-D Lightning Mapping Array applied to imaging of entire lightning flashes. An interferometric cross-correlation technique is applied in our system to compute windowed two-sensor time differences with submicrosecond time resolution before TOA is used for source location. Compared to previously reported LF lightning location systems, our system captures many more LF sources. This is due mainly to the improved mapping of continuous lightning processes by using this type of hybrid interferometry/TOA processing method. We show with five station measurements that the array detects and maps different lightning processes, such as stepped and dart leaders, during both in-cloud and cloud-to-ground flashes. Lightning images mapped by our LF system are remarkably similar to those created by VHF mapping systems, which may suggest some special links between LF and VHF emission during lightning processes.

Common effect of the mucus transferred during mating in two dart-shooting snail species from different families.

PubMed

Kimura, Kazuki; Chiba, Satoshi; Koene, Joris M

2014-04-01

Several taxa of pulmonate land snails exhibit a conspicuous mating behaviour, the shooting of so-called love darts. During mating, such land snail species stab a mating partner with a mucus-coated dart. It has previously been shown that the sperm donor physiologically influences the sperm recipient via the mucus covering the dart and thereby decreases the number of sperm digested by the recipient. However, the generality of this effect of the dart's mucus is unclear, because almost all the previous studies on the effect of the mucus used the brown garden snail Cornu aspersum from the family Helicidae. Therefore, the relationship between the acquisition of the mucus effect on the recipient and the evolution of the dart itself, and its mucus, is still open to debate. To test the commonality of the physiological effect of the dart mucus, we examined this in Euhadra peliomphala, a species from the Bradybaenidae family, and compared our findings with the results of previous work using C. aspersum. Our experiments showed that in E. peliomphala, the dart mucus had a physiological effect and lowered the accessibility of the gametolytic organ, as found in C. aspersum. This indicates that in various dart-bearing species the mucus from the dart glands targets the same organ and that the inhibition of sperm digestion has played a crucial role in the evolution of the dart and its mucus.

Integrated corridor management transit vehicle real-time data demonstration: Dallas case study

DOT National Transportation Integrated Search

2014-12-01

As part of the U.S. Department of Transportations Integrated Corridor Management (ICM) Initiative, Dallas Area Rapid Transit (DART) purchased new automatic passenger counter (APC) technology for its Red and Orange line light rail vehicles to provi...

Integrated e-Health approach based on vascular ultrasound and pulse wave analysis for asymptomatic atherosclerosis detection and cardiovascular risk stratification in the community.

PubMed

Santana, Daniel Bia; Zócalo, Yanina A; Armentano, Ricardo L

2012-03-01

New strategies are urgently needed to identify subjects at increased risk of atherosclerotic cardiovascular disease (ACVD) development or complications. A National Public University Center (CUiiDARTE) was created in Uruguay, based on six main pillars: 1) integration of experts in different disciplines and creation of multidisciplinary teams, 2) incidence in public and professional education programs to give training in the use of new technologies and to shift the focus from ACVD treatment to disease prevention, 3) implementation of free vascular studies in the community (distributed rather than centralized healthcare), 4) innovation and application of e-Health and noninvasive technology and approaches, 5) design and development of a biomedical approach to determine the target population and patient workflow, and 6) improvement in individual risk estimation and differentiation between aging and ACVD-related arterial changes using population-based epidemiological and statistical patient-specific models. This work describes main features of CUiiDARTE project implementation, the scientific and technological steps and innovations done for individual risk stratification, and sub-clinical ACVD diagnosis. © 2012 IEEE

Characterization of Printing Inks Using DART-Q-TOF-MS and Attenuated Total Reflectance (ATR) FTIR.

PubMed

Williamson, Rhett; Raeva, Anna; Almirall, Jose R

2016-05-01

The rise in improved and widely accessible printing technology has resulted in an interest to develop rapid and minimally destructive chemical analytical techniques that can characterize printing inks for forensic document analysis. Chemical characterization of printing inks allows for both discrimination of inks originating from different sources and the association of inks originating from the same source. Direct analysis in real-time mass spectrometry (DART-MS) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) were used in tandem to analyze four different classes of printing inks: inkjets, toners, offset, and intaglio. A total of 319 samples or ~ 80 samples from each class were analyzed directly on a paper substrate using the two methods. DART-MS was found to characterize the semi-volatile polymeric vehicle components, while ATR-FTIR provided chemical information associated with the bulk components of these inks. Complimentary data results in improved discrimination when both techniques are used in succession resulting in >96% discrimination for all toners, 95% for all inkjets, >92% for all offset, and >54% for all intaglio inks. © 2016 American Academy of Forensic Sciences.

DART: Tools and Support for Ensemble Data Assimilation Research, Operations, and Education

NASA Astrophysics Data System (ADS)

Hoar, T. J.; Anderson, J. L.; Collins, N.; Raeder, K.; Kershaw, H.; Romine, G. S.; Mizzi, A. P.; Chatterjee, A.; Karspeck, A. R.; Zarzycki, C. M.; Ha, S. Y.; Barre, J.; Gaubert, B.

2014-12-01

The Data Assimilation Research Testbed (DART) is a community facility for ensemble data assimilation developed and supported by the National Center for Atmospheric Research. DART provides a comprehensive suite of software, documentation, examples and tutorials that can be used for ensemble data assimilation research, operations, and education. Scientists and software engineers from the Data Assimilation Research Section at NCAR are available to actively support DART users who want to use existing DART products or develop their own new applications. Current DART users range from university professors teaching data assimilation, to individual graduate students working with simple models, through national laboratories doing operational prediction with large state-of-the-art models. DART runs efficiently on many computational platforms ranging from laptops through thousands of cores on the newest supercomputers. This poster focuses on several recent research activities using DART with geophysical models. First, DART is being used with the Community Atmosphere Model Spectral Element (CAM-SE) and Model for Prediction Across Scales (MPAS) global atmospheric models that support locally enhanced grid resolution. Initial results from ensemble assimilation with both models are presented. DART is also being used to produce ensemble analyses of atmospheric tracers, in particular CO, in both the global CAM-Chem model and the regional Weather Research and Forecast with chemistry (WRF-Chem) model by assimilating observations from the Measurements of Pollution in the Troposphere (MOPITT) and Infrared Atmospheric Sounding Interferometer (IASI) instruments. Results from ensemble analyses in both models are presented. An interface between DART and the Community Atmosphere Biosphere Land Exchange (CABLE) model has been completed and ensemble land surface analyses with DART/CABLE will be discussed. Finally, an update on ensemble analyses in the fully-coupled Community Earth System (CESM) is presented. The poster includes instructions on how to get started using DART for research or educational applications.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prentice, John K.; Gardner, David Randall

A methodology was developed for computing the probability that the sensor dart for the 'Near Real-Time Site Characterization for Assured HDBT Defeat' Grand-Challenge LDRD project will survive deployment over a forested region. The probability can be decomposed into three approximately independent probabilities that account for forest coverage, branch density and the physics of an impact between the dart and a tree branch. The probability that a dart survives an impact with a tree branch was determined from the deflection induced by the impact. If a dart that was deflected so that it impacted the ground at an angle of attackmore » exceeding a user-specified, threshold value, the dart was assumed to not survive the impact with the branch; otherwise it was assumed to have survived. A computer code was developed for calculating dart angle of attack at impact with the ground and a Monte Carlo scheme was used to calculate the probability distribution of a sensor dart surviving an impact with a branch as a function of branch radius, length, and height from the ground. Both an early prototype design and the current dart design were used in these studies. As a general rule of thumb, it we observed that for reasonably generic trees and for a threshold angle of attack of 5{sup o} (which is conservative for dart survival), the probability of reaching the ground with an angle of attack less than the threshold is on the order of 30% for the prototype dart design and 60% for the current dart design, though these numbers should be treated with some caution.« less

Improvements in NOAA's Operational Tsunameter Network since December 2004

NASA Astrophysics Data System (ADS)

Bouchard, R.; Kohler, C.; McArthur, S.; Burnett, W. H.; Wells, W. I.; Luke, R.

2009-12-01

In December 2004 during the devastating Sumatran Tsunami, the National Oceanic and Atmospheric Administration (NOAA) had five tsunameter stations established in the North Pacific Ocean and one in the South Pacific Ocean operated and maintained by NOAA’s National Data Buoy Center (NDBC). The original six tsunameters employed the technology of the first generation Deep-ocean Assessment and Reporting of Tsunamis (DART I) developed by NOAA’s Pacific Marine Environmental Laboratory (PMEL) and successfully transitioned to NDBC in 2003. The technology consists of a Bottom Pressure Recorder (BPR) that makes pressure measurements near the sea-floor and a surface buoy. It takes less than three minutes for data to get from the BPR, which can reside to depths of 6000 m, to users. The BPR contains a tsunami detection algorithm that will place the BPR in rapid reporting mode(also know as Event Mode). The two most profound improvements to the network were its expansion to 39 stations and the transition and upgrade to the second generation DART II systems. In the aftermath of the Sumatran Tsunami, NOAA expanded the network to 39 stations to bolster the US tsunami warning system by providing coastal communities in the Pacific, Atlantic, Caribbean and the Gulf of Mexico with faster and more accurate tsunami warnings. Cooperating NOAA offices selected the sites in consultation with the US Geological Survey and other interested parties. Since their initial establishment, NDBC has relocated some stations to improve data availability by reducing the risks of vessel collision, extreme winds, seas, and currents. NDBC completed the network in March 2008. During the expansion of the NOAA network, NDBC assisted several countries in the deploying and distributing data from their own DART II tsunameters. NDBC completed the upgraded of all stations to the DART II systems by the end of 2007. The significant capability fielded by the DART II technology was the bi-directional communications between the BPR and the TWCs via the surface buoy. This capability allows the TWCs to set the tsunameters in Event Mode (known as a Manual Trigger) in anticipation of the passage of a tsunami. The TWCs can also retrieve the full-resolution data in one-hour increments directly from the BPR and set the tsunami detection threshold. A further upgrade being fielded in 2009 and 2010 allows the TWCs to interrupt an ongoing Event Mode and restart the Event Mode to extend the rapid reporting period and to identify Manual Triggers from the message identifier rather than from an artificial 100 mm addition to water-column heights. Improvements occurred in data distribution. In 2008, in response to greater participation by the international community in the application of the tsunameter data, NDBC moved towards standardizing its tsunameter data messages and bulletins. Further improvements include evaluation of the use of the Easy-To-Deploy DART systems and autonomous vehicles for rapid mitigation response for failed stations and the development of international standardization of data message formats. Emphasis has been placed on mooring failure analysis and investigations into improving mooring reliability and subsequent data availability.

Detection of nicotine as an indicator of tobacco smoke by direct analysis in real time (DART) tandem mass spectrometry

NASA Astrophysics Data System (ADS)

Kuki, Ákos; Nagy, Lajos; Nagy, Tibor; Zsuga, Miklós; Kéki, Sándor

2015-01-01

The residual tobacco smoke contamination (thirdhand smoke, THS) on the clothes of a smoker was examined by direct analysis in real time (DART) mass spectrometry. DART-MS enabled sensitive and selective analysis of nicotine as the indicator of tobacco smoke pollution. Tandem mass spectrometric (MS/MS) experiments were also performed to confirm the identification of nicotine. Transferred thirdhand smoke originated from the fingers of a smoker onto other objects was also detected by DART mass spectrometry. DART-MS/MS was utilized for monitoring the secondhand tobacco smoke (SHS) in the air of the laboratory using nicotine as an indicator. To the best of our knowledge, this is the first report on the application of DART-MS and DART-MS/MS to the detection of thirdhand smoke and to the monitoring of secondhand smoke.

Characterization of mustard seeds and paste by DART ionization with time-of-flight mass spectrometry.

PubMed

Prchalová, Jana; Kovařík, František; Ševčík, Rudolf; Čížková, Helena; Rajchl, Aleš

2014-09-01

Direct analysis in real time (DART) is a novel technique with great potential for rapid screening analysis. The DART ionization method coupled with high-resolution time-of-flight mass spectrometry (TOF-MS) has been used for characterization of mustard seeds and table mustard. The possibility to use DART to analyse glucosinolates was confirmed on determination of sinalbin (4-hydroxybenzyl glucosinolate). The DART-TOF-MS method was optimized and validated. A set of samples of mustard seeds and mustard products was analyzed. High-performance liquid chromatography and DART-TOF-MS were used to determine glucosinolates in mustard seeds and compared. The correlation equation between these methods was DART = 0.797*HPLC + 6.987, R(2)  = 0.972. The DART technique seems to be a suitable method for evaluation of the quality of mustard seeds and mustard products. Copyright © 2014 John Wiley & Sons, Ltd.

Open source software integrated into data services of Japanese planetary explorations

NASA Astrophysics Data System (ADS)

Yamamoto, Y.; Ishihara, Y.; Otake, H.; Imai, K.; Masuda, K.

2015-12-01

Scientific data obtained by Japanese scientific satellites and lunar and planetary explorations are archived in DARTS (Data ARchives and Transmission System). DARTS provides the data with a simple method such as HTTP directory listing for long-term preservation while DARTS tries to provide rich web applications for ease of access with modern web technologies based on open source software. This presentation showcases availability of open source software through our services. KADIAS is a web-based application to search, analyze, and obtain scientific data measured by SELENE(Kaguya), a Japanese lunar orbiter. KADIAS uses OpenLayers to display maps distributed from Web Map Service (WMS). As a WMS server, open source software MapServer is adopted. KAGUYA 3D GIS (KAGUYA 3D Moon NAVI) provides a virtual globe for the SELENE's data. The main purpose of this application is public outreach. NASA World Wind Java SDK is used to develop. C3 (Cross-Cutting Comparisons) is a tool to compare data from various observations and simulations. It uses Highcharts to draw graphs on web browsers. Flow is a tool to simulate a Field-Of-View of an instrument onboard a spacecraft. This tool itself is open source software developed by JAXA/ISAS, and the license is BSD 3-Caluse License. SPICE Toolkit is essential to compile FLOW. SPICE Toolkit is also open source software developed by NASA/JPL, and the website distributes many spacecrafts' data. Nowadays, open source software is an indispensable tool to integrate DARTS services.

Performance Evaluation of a UWB-RFID System for Potential Space Applications

NASA Technical Reports Server (NTRS)

Phan, Chan T.; Arndt, D.; Ngo, P.; Gross, J.; Ni, Jianjun; Rafford, Melinda

2006-01-01

This talk presents a brief overview of the ultra-wideband (UWB) RFID system with emphasis on the performance evaluation of a commercially available UWB-RFID system. There are many RFID systems available today, but many provide just basic identification for auditing and inventory tracking. For applications that require high precision real time tracking, UWB technology has been shown to be a viable solution. The use of extremely short bursts of RF pulses offers high immunity to interference from other RF systems, precise tracking due to sub-nanosecond time resolution, and robust performance in multipath environments. The UWB-RFID system Sapphire DART (Digital Active RFID & Tracking) will be introduced in this talk. Laboratory testing using Sapphire DART is performed to evaluate its capability such as coverage area, accuracy, ease of operation, and robustness. Performance evaluation of this system in an operational environment (a receiving warehouse) for inventory tracking is also conducted. Concepts of using the UWB-RFID technology to track astronauts and assets are being proposed for space exploration.

On the mechanism of X-ray production by dart leaders of lightning flashes

NASA Astrophysics Data System (ADS)

Cooray, Vernon; Dwyer, Joseph; Rakov, V.; Rahman, Mahbubur

2010-07-01

Radiation with energies up to about 250 keV associated with the dart leader phase of rocket-triggered lightning were reported by Dwyer et al. (2004). The mechanism of X-ray generation by dart leaders, however, is unknown at present. Recently, Cooray et al. (2009a) developed physical concepts and mathematical techniques necessary to calculate the electric field associated with the tip of dart leaders. We have utilized the results of these calculations together with the energy dependent frictional force on electrons, as presented by Moss et al. (2006), to evaluate the maximum energy an electron will receive in accelerating in the dart-leader-tip electric field. The main assumptions made in performing the calculations are: (a) the dart leader channel is straight and vertical; (b) the path of the electrons are straight inside the channel; and (c) the decay of the channel temperature is uniform along the length of the dart leader. In the calculation, we have taken into account the fact that the electric field is changing both in space and time and that the gas in the defunct return stroke channel is at atmospheric pressure and at elevated temperature (i.e. reduced gas density). The results of the calculation show that for a given dart leader current there is a critical defunct-return-stroke-channel temperature above which the cold electron runaway becomes feasible. For a typical dart leader, this temperature is around 2500 K. This critical temperature decreases with increase in dart leader current. Since the temperature of the defunct return stroke channel may lie in the range of 2000-4000 K, the results show that the electric field at the tip of dart leaders is capable of accelerating electrons to MeV energy levels.

High level of sperm competition may increase transfer of accessory gland products carried by the love dart of land snails.

PubMed

Lodi, Monica; Staikou, Alexandra; Janssen, Ruben; Koene, Joris M

2017-12-01

Postcopulatory adaptations that increase reproductive success compared to rivals, like the transfer of accessory gland products that promote paternity, are common when sperm competition occurs among males. In land snails, the dart shooting behavior and its adaptive significance, in promoting individual fitness through enhanced paternity of the successful dart shooter, have been considered such an adaptation. The fitness result gained is mediated by the transfer of mucus components on the love dart capable of altering the physiology of the receiver's reproductive tract. In this context, dart shooting and mucus transfer could be considered as processes targeted by sexual selection. While the effect of dart mucus is beneficial for the dart user, so far it has remained unknown whether its transport is greater when snails experience a higher level of sperm competition. Here, we report results of a study on inter- and intraspecific variations of dart and mucus gland morphometry, considered to be traits reflecting the ability of snails to adjust the production and transfer of mucus under varying sperm competition scenarios. We investigated four populations with different densities from four dart-bearing species, Arianta arbustorum , Cepaea nemoralis , Cornu aspersum, and Helix lucorum . The results indicate that different adaptations of these traits occur among the studied species that all seem to achieve the same goal of transferring more mucus when sperm competition is higher. For example, the presence of longer and more branched mucous glands or an increase in dart surface most likely reflect increased mucus production and enhanced ability of mucus transport, respectively. Interestingly, the species for which the use of the dart is reported to be facultative, A. arbustorum , did not show any variation among the examined traits. To conclude, sexual selection in the form of sperm competition intensity seems to be an important selective force for these simultaneously hermaphroditic dart-bearing snails, driving differences in sexual traits.

Reflective array modeling for reflective and directional SAW transducers.

PubMed

Morgan, D P

1998-01-01

This paper presents a new approximate method for analyzing reflective SAW transducers, with much of the convenience of the coupled-mode (COM) method but with better accuracy. Transduction accuracy is obtained by incorporating the accurate electrostatic solution, giving for example correct harmonics, and allowance for electrode width variation, in a simple manner. Results are shown for a single-electrode transducer, Natural SPUDT and DART SPUDT, each using theoretically derived parameters. In contrast to the COM, the RAM can give accurate results for short or withdrawal-weighted transducers and for wide analysis bandwidth.

Argon direct analysis in real time mass spectrometry in conjunction with makeup solvents: a method for analysis of labile compounds.

PubMed

Yang, Hongmei; Wan, Debin; Song, Fengrui; Liu, Zhiqiang; Liu, Shuying

2013-02-05

Helium direct analysis in real time (He-DART) mass spectrometry (MS) analysis of labile compounds usually tends to be challenging because of the occurrence of prominent fragmentation, which obscures the assigning of an ion to an independent species or merely a fragment in a mixture. In the present work, argon DART (Ar-DART) MS in conjunction with makeup solvents has been demonstrated to analyze a variety of labile compounds including nucleosides, alkaloids, glucose, and other small molecules. The results presented here confirm that Ar-DART can generate significantly less energetic ions than conventional He-DART and is able to produce the intact molecular ions with little or no fragmentation in both positive and negative ion modes. Adding a makeup solvent (absolute ethyl alcohol, methanol, fluorobenzene, or acetone) to the argon gas stream at the exit of the DART ion source can result in 1-2 orders of magnitude increase in detection signals. The sensitivity attainable by Ar-DART was found to be comparable to that by He-DART. The investigation of influence of solvents improves our understanding of the fundamental desorption and ionization processes in DART. The practical application of this rapid and high throughput method is demonstrated by the successful analysis of a natural product (Crude Kusnezoff Monkshood) extract, demonstrating the great potential in mixture research.

The Effects of Self-Esteem and Task Perception on Goal Setting, Efficacy, and Task Performance.

ERIC Educational Resources Information Center

Tang, Thomas Li-Ping; Reynolds, David Bryan

This study examined the effects of self-esteem and task perception on goal setting, efficacy, and task performance in 52 recreational dart throwers who were members of two dart organizations. Task perception was manipulated by asking each dart thrower to compete against self, a difficult competitor, and an easy competitor on the same dart game.…

Motion-plane dependency of the range of dart throw motion and the effects of tendon action due to finger extrinsic muscles during the motion.

PubMed

Mitsukane, Masahiro; Sekiya, Noboru; Kamono, Arinori; Nakabo, Tohru

2018-03-01

[Purpose] To clarify the motion-plane dependency of the range of dart throw motion and the effects of tendon action due to long finger flexors and extensors during the motion. [Subjects and Methods] Forty healthy subjects attended the experiment, and the active range of wrist motion in seven motion planes was measured with an originally designed apparatus. [Results] The reliability of the measurement was acceptable. The range of dart throw motion depended on the motion planes, with a maximum at around the motion plane of 45° from the sagittal plane (45° of pronation). The tendon action of long finger muscles was shown in dart throw motion except in 45° of pronation. [Conclusion] Motion-plane dependency of the range of dart throw motion exists in healthy subjects. The absence of tendon action due to finger extrinsic muscles in dart throw motion at 45° might be one of the causes of the advantage of dart throw motion.

Source identification of western Oregon Douglas-fir wood cores using mass spectrometry and random forest classification.

PubMed

Finch, Kristen; Espinoza, Edgard; Jones, F Andrew; Cronn, Richard

2017-05-01

We investigated whether wood metabolite profiles from direct analysis in real time (time-of-flight) mass spectrometry (DART-TOFMS) could be used to determine the geographic origin of Douglas-fir wood cores originating from two regions in western Oregon, USA. Three annual ring mass spectra were obtained from 188 adult Douglas-fir trees, and these were analyzed using random forest models to determine whether samples could be classified to geographic origin, growth year, or growth year and geographic origin. Specific wood molecules that contributed to geographic discrimination were identified. Douglas-fir mass spectra could be differentiated into two geographic classes with an accuracy between 70% and 76%. Classification models could not accurately classify sample mass spectra based on growth year. Thirty-two molecules were identified as key for classifying western Oregon Douglas-fir wood cores to geographic origin. DART-TOFMS is capable of detecting minute but regionally informative differences in wood molecules over a small geographic scale, and these differences made it possible to predict the geographic origin of Douglas-fir wood with moderate accuracy. Studies involving DART-TOFMS, alone and in combination with other technologies, will be relevant for identifying the geographic origin of illegally harvested wood.

Achievements and prospects of genomics-assisted breeding in three legume crops of the semi-arid tropics

USDA-ARS?s Scientific Manuscript database

Advances in sequencing and genotyping technologies have enabled generation of several thousand markers including SSRs, SNPs, DArTs, hundreds of thousands transcript reads and BAC-end sequences in chickpea, pigeonpea and groundnut, three major legume crops of the semi-arid tropics. Comprehensive tran...

Using Minimum Energy in Ireland's Schools

ERIC Educational Resources Information Center

Dolan, John

2007-01-01

Since 1998, the Department of Education and Science has been using a process of design, awareness, research and technology known as DART to develop energy efficiency in educational buildings. Currently all primary schools built in accordance with the Department's primary schools technical guidance documents are capable of being up to 2.3 times…

Direct analysis in real time--a critical review on DART-MS.

PubMed

Gross, Jürgen H

2014-01-01

Direct analysis in real time mass spectrometry (DART-MS) has become an established technique for rapid mass spectral analysis of a large variety of samples. DART-MS is capable of analyzing the sample at atmospheric pressure, essentially in the open laboratory environment. DART-MS can be applied to compounds that have been deposited or adsorbed on to surfaces or that are being desorbed therefrom into the atmosphere. This makes DART-MS suitable and well-known for analysis of ingredients of plant materials, pesticide monitoring on vegetables, forensic and safety applications such as screening for traces of explosives, warfare agents, or illicit drugs on luggage, clothes, or bank notes, etc. DART can also be used for analysis of either solid or liquid bulk materials, as may be required in quality control, or to quickly investigate the identity of a compound from chemical synthesis. Even living organisms can be subjected to DART-MS. Driven by different needs in analytical practice, the combination of the DART ionization source and interface can be configured in multiple geometries and with various accessories to adapt the setup as required. Analysis by DART-MS relies on some sort of gas-phase ionization mechanism. In DART, initial generation of the ionizing species is by use of a corona discharge in a pure helium atmosphere which delivers excited helium atoms that, upon their release into the atmosphere, will initiate a cascade of gas-phase reactions. In the end, this results in reagent ions created from atmospheric water or (solvent) vapor in the vicinity of the surface subject to analysis where they effect a chemical ionization process. DART ionization processes may generate positive or negative ions, predominantly even-electron species, but odd-electron species do also occur. The prevailing process of analyte ion formation from a given sample is highly dependent on analyte properties.

Some new features of Direct Analysis in Real Time mass spectrometry utilizing the desorption at an angle option.

PubMed

Chernetsova, Elena S; Revelsky, Alexander I; Morlock, Gertrud E

2011-08-30

The present study is a first step towards the unexplored capabilities of Direct Analysis in Real Time (DART) mass spectrometry (MS) arising from the possibility of the desorption at an angle: scanning analysis of surfaces, including the coupling of thin-layer chromatography (TLC) with DART-MS, and a more sensitive analysis due to the preliminary concentration of analytes dissolved in large volumes of liquids on glass surfaces. In order to select the most favorable conditions for DART-MS analysis, proper positioning of samples is important. Therefore, a simple and cheap technique for the visualization of the impact region of the DART gas stream onto a substrate was developed. A filter paper or TLC plate, previously loaded with the analyte, was immersed in a derivatization solution. On this substrate, owing to the impact of the hot DART gas, reaction of the analyte to a colored product occurred. An improved capability of detection of DART-MS for the analysis of liquids was demonstrated by applying large volumes of model solutions of coumaphos into small glass vessels and drying these solutions prior to DART-MS analysis under ambient conditions. This allowed the introduction of, by up to more than two orders of magnitude, increased quantities of analyte compared with the conventional DART-MS analysis of liquids. Through this improved detectability, the capabilities of DART-MS in trace analysis could be strengthened. Copyright © 2011 John Wiley & Sons, Ltd.

Improvement in ionization efficiency of direct analysis in real time-mass spectrometry (DART-MS) by corona discharge.

PubMed

Sekimoto, Kanako; Sakakura, Motoshi; Kawamukai, Takatomo; Hike, Hiroshi; Shiota, Teruhisa; Usui, Fumihiko; Bando, Yasuhiko; Takayama, Mitsuo

2016-08-02

Herein it is shown that a combination of direct analysis in real time (DART) with a corona discharge system consisting of only a needle electrode easily improves DART ionization efficiency. Positive and negative DC corona discharges led to a formation of abundant excited helium atoms as well as the reactant ions H3O(+)(H2O)n and O2˙(-) in the DART analyte ionization area. These phenomena resulted in an increase in the absolute intensities of (de)protonated analytes by a factor of 2-20 over conventional DART. The other analyte ions detected in this corona-DART system (i.e., molecular ions, fragment ions, oxygenated (de)protonated analytes, dehydrogenated deprotonated analytes, and negative ion adducts) were quite similar to those obtained from DART alone. This indicates a lack of side reactions due to the corona discharge. The change in the relative intensities of individual analyte-related ions due to the combination of a corona discharge system with DART suggests that there is no effect of the abundant excited helium in the analyte ionization area on the fragmentation processes or enhancement of oxidation due to hydroxyl radicals HO˙. Furthermore, it was found that the corona-DART combination can be applied to the highly sensitive analysis of n-alkanes, in which the alkanes are ionized as positive ions via hydride abstraction and oxidation, independent of the type of alkane or the mass spectrometer used.

The direct cost of traumatic secretion transfer in hermaphroditic land snails: individuals stabbed with a love dart decrease lifetime fecundity

PubMed Central

Kimura, Kazuki; Chiba, Satoshi

2015-01-01

Several taxa of simultaneously hermaphroditic land snails exhibit a conspicuous mating behaviour, the so-called shooting of love darts. During mating, such land snail species transfer a specific secretion by stabbing a mating partner's body with the love dart. It has been shown that sperm donors benefit from this traumatic secretion transfer, because the secretions manipulate the physiology of a sperm recipient and increase the donors' fertilization success. However, it is unclear whether reception of dart shooting is costly to the recipients. Therefore, the effect of sexual conflict and antagonistic arms races on the evolution of traumatic secretion transfer in land snails is still controversial. To examine this effect, we compared lifetime fecundity and longevity between the individuals that received and did not receive dart shooting from mating partners in Bradybaena pellucida. Our experiments showed that the dart-receiving snails suffered reduction in lifetime fecundity and longevity. These results suggest that the costly mating behaviour, dart shooting, generates conflict between sperm donors and recipients and that sexually antagonistic arms races have contributed to the diversification of the morphological and behavioural traits relevant to dart shooting. Our findings also support theories suggesting a violent escalation of sexual conflict in hermaphroditic animals. PMID:25761713

Raymond Dart as a pioneering primatologist.

PubMed

Strkalj, G; Tobias, P V

2008-01-01

Raymond Dart is best known today for his groundbreaking research in palaeoanthropology. It is often forgotten, however, that Dart was a scientist of many interests, who made significant contributions to various disciplines. One of these is the study of living non-human primates. Dart became aware of the importance of primate studies and their relevance for research in other disciplines early in his career. In the late 1920s Dart established a colony of captive baboons in the Anatomy Department, University of the Witwatersrand, Johannesburg. On these animals, members of his Department, most notably Joseph Gillman and Christine Gilbert, carried out a number of significant behavioural and endocrinological researches. In 1930, as a member of an Italian Scientific Expedition, Dart was involved in hunting a mountain gorilla (for research purposes). He was also active in primate field studies. In 1957 he and Phillip Tobias founded a Witwatersrand University Uganda Gorilla Research Unit for the study of the Virunga mountain gorillas. The unit produced pioneering studies, conducted by Jill Donisthorpe, on the behaviour of these primates in their natural habitat. At the same time Dart was actively engaged in conservation of the mountain gorillas. He also studied South African chacma baboons in the wild. In the field of primate studies Raymond Dart figures prominently as a pioneering catalyst as well as researcher and conservationist.

16 CFR 1306.1 - Scope and application.

Code of Federal Regulations, 2011 CFR

2011-01-01

... HAZARDOUS LAWN DARTS § 1306.1 Scope and application. (a) In this part 1306, the Commission declares lawn darts, described in § 1306.3, to be banned hazardous products. (b) Lawn darts and similar products that...

16 CFR 1306.1 - Scope and application.

Code of Federal Regulations, 2010 CFR

2010-01-01

... HAZARDOUS LAWN DARTS § 1306.1 Scope and application. (a) In this part 1306, the Commission declares lawn darts, described in § 1306.3, to be banned hazardous products. (b) Lawn darts and similar products that...

16 CFR 1306.1 - Scope and application.

Code of Federal Regulations, 2014 CFR

2014-01-01

... HAZARDOUS LAWN DARTS § 1306.1 Scope and application. (a) In this part 1306, the Commission declares lawn darts, described in § 1306.3, to be banned hazardous products. (b) Lawn darts and similar products that...

16 CFR 1306.1 - Scope and application.

Code of Federal Regulations, 2012 CFR

2012-01-01

... HAZARDOUS LAWN DARTS § 1306.1 Scope and application. (a) In this part 1306, the Commission declares lawn darts, described in § 1306.3, to be banned hazardous products. (b) Lawn darts and similar products that...

Application of the DART Code for the Assessment of Advanced Fuel Behavior

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rest, J.; Totev, T.

2007-07-01

The Dispersion Analysis Research Tool (DART) code is a dispersion fuel analysis code that contains mechanistically-based fuel and reaction-product swelling models, a one dimensional heat transfer analysis, and mechanical deformation models. DART has been used to simulate the irradiation behavior of uranium oxide, uranium silicide, and uranium molybdenum aluminum dispersion fuels, as well as their monolithic counterparts. The thermal-mechanical DART code has been validated against RERTR tests performed in the ATR for irradiation data on interaction thickness, fuel, matrix, and reaction product volume fractions, and plate thickness changes. The DART fission gas behavior model has been validated against UO{sub 2}more » fission gas release data as well as measured fission gas-bubble size distributions. Here DART is utilized to analyze various aspects of the observed bubble growth in U-Mo/Al interaction product. (authors)« less

Difficult airway response team: a novel quality improvement program for managing hospital-wide airway emergencies.

PubMed

Mark, Lynette J; Herzer, Kurt R; Cover, Renee; Pandian, Vinciya; Bhatti, Nasir I; Berkow, Lauren C; Haut, Elliott R; Hillel, Alexander T; Miller, Christina R; Feller-Kopman, David J; Schiavi, Adam J; Xie, Yanjun J; Lim, Christine; Holzmueller, Christine; Ahmad, Mueen; Thomas, Pradeep; Flint, Paul W; Mirski, Marek A

2015-07-01

Difficult airway cases can quickly become emergencies, increasing the risk of life-threatening complications or death. Emergency airway management outside the operating room is particularly challenging. We developed a quality improvement program-the Difficult Airway Response Team (DART)-to improve emergency airway management outside the operating room. DART was implemented by a team of anesthesiologists, otolaryngologists, trauma surgeons, emergency medicine physicians, and risk managers in 2005 at The Johns Hopkins Hospital in Baltimore, Maryland. The DART program had 3 core components: operations, safety, and education. The operations component focused on developing a multidisciplinary difficult airway response team, standardizing the emergency response process, and deploying difficult airway equipment carts throughout the hospital. The safety component focused on real-time monitoring of DART activations and learning from past DART events to continuously improve system-level performance. This objective entailed monitoring the paging system, reporting difficult airway events and DART activations to a Web-based registry, and using in situ simulations to identify and mitigate defects in the emergency airway management process. The educational component included development of a multispecialty difficult airway curriculum encompassing case-based lectures, simulation, and team building/communication to ensure consistency of care. Educational materials were also developed for non-DART staff and patients to inform them about the needs of patients with difficult airways and ensure continuity of care with other providers after discharge. Between July 2008 and June 2013, DART managed 360 adult difficult airway events comprising 8% of all code activations. Predisposing patient factors included body mass index >40, history of head and neck tumor, prior difficult intubation, cervical spine injury, airway edema, airway bleeding, and previous or current tracheostomy. Twenty-three patients (6%) required emergent surgical airways. Sixty-two patients (17%) were stabilized and transported to the operating room for definitive airway management. There were no airway management-related deaths, sentinel events, or malpractice claims in adult patients managed by DART. Five in situ simulations conducted in the first program year improved DART's teamwork, communication, and response times and increased the functionality of the difficult airway carts. Over the 5-year period, we conducted 18 airway courses, through which >200 providers were trained. DART is a comprehensive program for improving difficult airway management. Future studies will examine the comparative effectiveness of the DART program and evaluate how DART has impacted patient outcomes, operational efficiency, and costs of care.

Difficult Airway Response Team: A Novel Quality Improvement Program for Managing Hospital-Wide Airway Emergencies

PubMed Central

Mark, Lynette J.; Herzer, Kurt R.; Cover, Renee; Pandian, Vinciya; Bhatti, Nasir I.; Berkow, Lauren C.; Haut, Elliott R.; Hillel, Alexander T.; Miller, Christina R.; Feller-Kopman, David J.; Schiavi, Adam J.; Xie, Yanjun J.; Lim, Christine; Holzmueller, Christine; Ahmad, Mueen; Thomas, Pradeep; Flint, Paul W.; Mirski, Marek A.

2015-01-01

Background Difficult airway cases can quickly become emergencies, increasing the risk of life-threatening complications or death. Emergency airway management outside the operating room is particularly challenging. Methods We developed a quality improvement program—the Difficult Airway Response Team (DART)—to improve emergency airway management outside the operating room. DART was implemented by a team of anesthesiologists, otolaryngologists, trauma surgeons, emergency medicine physicians, and risk managers in 2005 at The Johns Hopkins Hospital in Baltimore, Maryland. The DART program had three core components: operations, safety, and education. The operations component focused on developing a multidisciplinary difficult airway response team, standardizing the emergency response process, and deploying difficult airway equipment carts throughout the hospital. The safety component focused on real-time monitoring of DART activations and learning from past DART events to continuously improve system-level performance. This objective entailed monitoring the paging system, reporting difficult airway events and DART activations to a web-based registry, and using in situ simulations to identify and mitigate defects in the emergency airway management process. The educational component included development of a multispecialty difficult airway curriculum encompassing case-based lectures, simulation, and team building/communication to ensure consistency of care. Educational materials were also developed for non-DART staff and patients to inform them about the needs of patients with difficult airways and ensure continuity of care with other providers after discharge. Results Between July 2008 and June 2013, DART managed 360 adult difficult airway events comprising 8% of all code activations. Predisposing patient factors included body mass index > 40, history of head and neck tumor, prior difficult intubation, cervical spine injury, airway edema, airway bleeding, and previous or current tracheostomy. Twenty-three patients (6%) required emergent surgical airways. Sixty-two patients (17%) were stabilized and transported to the operating room for definitive airway management. There were no airway management-related deaths, sentinel events, or malpractice claims in adult patients managed by DART. Five in situ simulations conducted in the first program year improved DART's teamwork, communication, and response times and increased the functionality of the difficult airway carts. Over the 5-year period, we conducted 18 airway courses, through which more than 200 providers were trained. Conclusions DART is a comprehensive program for improving difficult airway management. Future studies will examine the comparative effectiveness of the DART program and evaluate how DART has impacted patient outcomes, operational efficiency, and costs of care. PMID:26086513

16 CFR § 1306.1 - Scope and application.

Code of Federal Regulations, 2013 CFR

2013-01-01

... BAN OF HAZARDOUS LAWN DARTS § 1306.1 Scope and application. (a) In this part 1306, the Commission declares lawn darts, described in § 1306.3, to be banned hazardous products. (b) Lawn darts and similar...

The direct cost of traumatic secretion transfer in hermaphroditic land snails: individuals stabbed with a love dart decrease lifetime fecundity.

PubMed

Kimura, Kazuki; Chiba, Satoshi

2015-04-07

Several taxa of simultaneously hermaphroditic land snails exhibit a conspicuous mating behaviour, the so-called shooting of love darts. During mating, such land snail species transfer a specific secretion by stabbing a mating partner's body with the love dart. It has been shown that sperm donors benefit from this traumatic secretion transfer, because the secretions manipulate the physiology of a sperm recipient and increase the donors' fertilization success. However, it is unclear whether reception of dart shooting is costly to the recipients. Therefore, the effect of sexual conflict and antagonistic arms races on the evolution of traumatic secretion transfer in land snails is still controversial. To examine this effect, we compared lifetime fecundity and longevity between the individuals that received and did not receive dart shooting from mating partners in Bradybaena pellucida. Our experiments showed that the dart-receiving snails suffered reduction in lifetime fecundity and longevity. These results suggest that the costly mating behaviour, dart shooting, generates conflict between sperm donors and recipients and that sexually antagonistic arms races have contributed to the diversification of the morphological and behavioural traits relevant to dart shooting. Our findings also support theories suggesting a violent escalation of sexual conflict in hermaphroditic animals. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

Maximizing photovoltaic power generation of a space-dart configured satellite

NASA Astrophysics Data System (ADS)

Lee, Dae Young; Cutler, James W.; Mancewicz, Joe; Ridley, Aaron J.

2015-06-01

Many small satellites are power constrained due to their minimal solar panel area and the eclipse environment of low-Earth orbit. As with larger satellites, these small satellites, including CubeSats, use deployable power arrays to increase power production. This presents a design opportunity to develop various objective functions related to energy management and methods for optimizing these functions over a satellite design. A novel power generation model was created, and a simulation system was developed to evaluate various objective functions describing energy management for complex satellite designs. The model uses a spacecraft-body-fixed spherical coordinate system to analyze the complex geometry of a satellite's self-induced shadowing with computation provided by the Open Graphics Library. As an example design problem, a CubeSat configured as a space-dart with four deployable panels is optimized. Due to the fast computation speed of the solution, an exhaustive search over the design space is used to find the solar panel deployment angles which maximize total power generation. Simulation results are presented for a variety of orbit scenarios. The method is extendable to a variety of complex satellite geometries and power generation systems.

Pneumatic dart delivery of tulathromycin in calves results in lower antimicrobial concentrations and increased biomarkers of stress and injection site inflammation compared to subcutaneous injection.

PubMed

Coetzee, Johann F; Kleinhenz, Michael D; Magstadt, Drew R; Cooper, Vickie L; Wulf, Larry W; Van Engen, Nicholas K; Smith, Joseph S; Rand, Nathan; KuKanich, Butch; Gorden, Patrick J

2018-06-05

Remote drug delivery (RDD) using pneumatic darts has become more prevalent in situations where cattle handling facilities are not available. The objective of this study was to compare the effect of pneumatic dart delivery and subcutaneous injection of tulathromycin on plasma pharmacokinetics and biomarkers of inflammation, stress, and muscle injury in calves. Twenty-three castrated-male Holstein calves, approximately 10 months of age with an average weight of 378 ± 6.49 kg, were randomly assigned to one of two groups. Calves in the RDD group (n=15) received 10 mL of tulathromycin (2.42 to 2.93 mg/kg) delivered into the left neck using a Type U 10.0 mL 1.9 cm 14 G Needle pneumatic dart administered with a breech loading projector. With the exception of one light weight calf that received 7 mL (2.53 mg/kg), calves in the injection group (INJ) (n=8) also received 10 mL of tulathromycin (2.34 to 2.68 mg/kg) administered as a single subcutaneous injection in the left neck using an 14 G, 1.9 cm needle and a 12 mL syringe. Serum tulathromycin, cortisol, creatine kinase (CK) and aspartate aminotransferase (AST) concentrations were determined in combination with other biomarkers of inflammation including mechanical nociceptive threshold (MNT), infrared thermography (IRT) and swelling at the injection site over 432 h after administration. Pneumatic darts failed to deliver the required dose of tulathromycin in 4 of 15 calves evidenced by heavier dart weights post-administration (24 g vs. 13.5 g). When these 4 calves were removed from the analysis, calves in the RDD group were found to have a smaller area under the tulathromycin concentration curve (AUC) (P=0.005) and faster clearance (P=0.025) compared to the INJ group. Furthermore, the RDD group recorded a greater difference in MNT between the treated and contralateral neck compared to the INJ group at 12 h (P= 0.016), 216 h (P= 0.024) and 288 h (P= 0.0494) after administration. Serum CK was elevated at 24 h (P= 0.03) and AST was greater at 24 h (P=0.024) and 48 h (P=0.037) after RDD. Serum cortisol concentrations were also greater at 0.5 h (P= 0.02) after RDD. These findings suggest that RDD is associated with reduced total body exposure to tulathromycin and increased acute stress, muscle damage and pain at the injection site. Furthermore, the failure of darts to consistently deliver antimicrobial therapy has a negative impact on the welfare of sick animals treated with RDD technologies.

Origin of year-long bean (Phaseolus dumosus Macfady, Fabaceae) from reticulated hybridization events between multiple Phaseolus species.

PubMed

Mina-Vargas, Angela M; McKeown, Peter C; Flanagan, Nicola S; Debouck, Daniel G; Kilian, Andrzej; Hodkinson, Trevor R; Spillane, Charles

2016-08-06

Improved understanding of the secondary gene pools of crops is essential for advancing genetic gain in breeding programmes. Common bean, Phaseolus vulgaris, is a staple crop with several wild relatives in its secondary gene pool. The year-long bean, P. dumosus, an important crop in Guatemala, is considered particularly closely related to P. vulgaris and a potential source of novel variation. However, the genetic diversity and relationship to other Phaseolus species of P. dumosus remain unclear. We conducted the first comprehensive investigation of P. dumosus genetic diversity using both nuclear and chloroplast genome markers. Our nuclear marker set included over 700 markers present within the Phaseolus DArT (Diversity Arrays Technology) array, which we applied to P. dumosus and other relatives of P. vulgaris (including every secondary gene pool species: P. acutifolius, P. albescens, P. coccineus and P. costaricensis). Phaseolus dumosus arose from hybridization of P. vulgaris and P. coccineus, followed by at least two later hybridizations with sympatric congener populations. Existing P. dumosus collections have low genetic diversity. The under-utilized crop P. dumosus has a complex hybrid origin. Further sampling in the region in which it arose may uncover additional germplasm for introgressing favourable traits into crops within the P. vulgaris gene pool. © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Direct analysis in real time--high resolution mass spectrometry as a valuable tool for the pharmaceutical drug development.

PubMed

Srbek, Jan; Klejdus, Bořivoj; Douša, Michal; Břicháč, Jiří; Stasiak, Pawel; Reitmajer, Josef; Nováková, Lucie

2014-12-01

In this study, direct analysis in real time-mass spectrometry (DART-MS) was assessed for the analysis of various pharmaceutical formulations with intention to summarize possible applications for the routine pharmaceutical development. As DART is an ambient ionization technique, it allows direct analysis of pharmaceutical samples in solid or liquid form without complex sample preparation, which is often the most time-consuming part of the analytical method. This makes the technique suitable for many application fields, including pharmaceutical drug development. DART mass spectra of more than twenty selected tablets and other common pharmaceutical formulations, i.e. injection solutions, ointments and suppositories developed in the pharmaceutical industry during several recent years are presented. Moreover, as thin-layer chromatography (TLC) is still very popular for the monitoring of the reactions in the synthetic chemistry, several substances were analyzed directly from the TLC plates to demonstrate the simplicity of the technique. Pure substance solutions were spotted onto a TLC plate and then analyzed with DART without separation. This was the first DART-MS study of pharmaceutical dosage forms using DART-Orbitrap combination. The duration of sample analysis by the DART-MS technique lasted several seconds, allowing enough time to collect sufficient number of data points for compound identification. The experimental setup provided excellent mass accuracy and high resolution of the mass spectra which allowed unambiguous identification of the compounds of interest. Finally, DART mass spectrometry was also used for the monitoring of the selected impurity distribution in the atorvastatin tablets. These measurements demonstrated DART to be robust ionization technique, which provided easy-to-interpret mass spectra for the broad range of compounds. DART has high-throughput potential for various types of pharmaceutical analyses and therefore eliminates the time for sample cleanup and chromatographic separation. Copyright © 2014 Elsevier B.V. All rights reserved.

Assessing direct analysis in real-time-mass spectrometry (DART-MS) for the rapid identification of additives in food packaging.

PubMed

Ackerman, L K; Noonan, G O; Begley, T H

2009-12-01

The ambient ionization technique direct analysis in real time (DART) was characterized and evaluated for the screening of food packaging for the presence of packaging additives using a benchtop mass spectrometer (MS). Approximate optimum conditions were determined for 13 common food-packaging additives, including plasticizers, anti-oxidants, colorants, grease-proofers, and ultraviolet light stabilizers. Method sensitivity and linearity were evaluated using solutions and characterized polymer samples. Additionally, the response of a model additive (di-ethyl-hexyl-phthalate) was examined across a range of sample positions, DART, and MS conditions (temperature, voltage and helium flow). Under optimal conditions, molecular ion (M+H+) was the major ion for most additives. Additive responses were highly sensitive to sample and DART source orientation, as well as to DART flow rates, temperatures, and MS inlet voltages, respectively. DART-MS response was neither consistently linear nor quantitative in this setting, and sensitivity varied by additive. All additives studied were rapidly identified in multiple food-packaging materials by DART-MS/MS, suggesting this technique can be used to screen food packaging rapidly. However, method sensitivity and quantitation requires further study and improvement.

A Belated Appreciation of Justin Dart (1930-2002).

ERIC Educational Resources Information Center

McCarthy, Henry

2003-01-01

This article highlights the life experiences of civil rights leader Justin Dart, Jr. and his transformation into a tireless advocate for disability rights. The author also recounts how his own prejudice and stereotyping almost prevented him from getting to know Dart. (GCP)

Fermilab DART run control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oleynik, G.; Engelfried, J.; Mengel, L.

1996-02-01

DART is the high speed, Unix based data acquisition system being developed by Fermilab in collaboration with seven High Energy Physics Experiments. This paper describes DART run control, which has been developed over the past year and is a flexible, distributed, extensible system for the control and monitoring of the data acquisition systems. The authors discuss the unique and interesting concepts of the run control and some of the experiences in developing it. They also give a brief update and status of the whole DART system.

Fermilab DART run control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oleynik, G.; Engelfried, J.; Mengel, L.

1995-05-01

DART is the high speed, Unix based data acquisition system being developed by Fermilab in collaboration with seven High Energy Physics Experiments. This paper describes DART run control, which has been developed over the past year and is a flexible, distributed, extensible system for the, control and monitoring of the data acquisition systems. We discuss the unique and interesting concepts of the run control and some of our experiences in developing it. We also give a brief update and status of the whole DART system.

Targeting HIV Reservoir in Infected CD4 T Cells by Dual-Affinity Re-targeting Molecules (DARTs) that Bind HIV Envelope and Recruit Cytotoxic T Cells

PubMed Central

Sloan, Derek D.; Lam, Chia-Ying Kao; Irrinki, Alivelu; Liu, Liqin; Tsai, Angela; Pace, Craig S.; Kaur, Jasmine; Murry, Jeffrey P.; Balakrishnan, Mini; Moore, Paul A.; Johnson, Syd; Nordstrom, Jeffrey L.; Cihlar, Tomas; Koenig, Scott

2015-01-01

HIV reservoirs and production of viral antigens are not eliminated in chronically infected participants treated with combination antiretroviral therapy (cART). Novel therapeutic strategies aiming at viral reservoir elimination are needed to address chronic immune dysfunction and non-AIDS morbidities that exist despite effective cART. The HIV envelope protein (Env) is emerging as a highly specific viral target for therapeutic elimination of the persistent HIV-infected reservoirs via antibody-mediated cell killing. Dual-Affinity Re-Targeting (DART) molecules exhibit a distinct mechanism of action via binding the cell surface target antigen and simultaneously engaging CD3 on cytotoxic T lymphocytes (CTLs). We designed and evaluated Env-specific DARTs (HIVxCD3 DARTs) derived from known antibodies recognizing diverse Env epitopes with or without broadly neutralizing activity. HIVxCD3 DARTs derived from PGT121, PGT145, A32, and 7B2, but not VRC01 or 10E8 antibodies, mediated potent CTL-dependent killing of quiescent primary CD4 T cells infected with diverse HIV isolates. Similar killing activity was also observed with DARTs structurally modified for in vivo half-life extension. In an ex vivo model using cells isolated from HIV-infected participants on cART, combinations of the most potent HIVxCD3 DARTs reduced HIV expression both in quiescent and activated peripheral blood mononuclear cell cultures isolated from HIV-infected participants on suppressive cART. Importantly, HIVxCD3 DARTs did not induce cell-to-cell virus spread in resting or activated CD4 T cell cultures. Collectively, these results provide support for further development of HIVxCD3 DARTs as a promising therapeutic strategy for targeting HIV reservoirs. PMID:26539983

Toward the Real-Time Tsunami Parameters Prediction

NASA Astrophysics Data System (ADS)

Lavrentyev, Mikhail; Romanenko, Alexey; Marchuk, Andrey

2013-04-01

Today, a wide well-developed system of deep ocean tsunami detectors operates over the Pacific. Direct measurements of tsunami-wave time series are available. However, tsunami-warning systems fail to predict basic parameters of tsunami waves on time. Dozens examples could be provided. In our view, the lack of computational power is the main reason of these failures. At the same time, modern computer technologies such as, GPU (graphic processing unit) and FPGA (field programmable gates array), can dramatically improve data processing performance, which may enhance timely tsunami-warning prediction. Thus, it is possible to address the challenge of real-time tsunami forecasting for selected geo regions. We propose to use three new techniques in the existing tsunami warning systems to achieve real-time calculation of tsunami wave parameters. First of all, measurement system (DART buoys location, e.g.) should be optimized (both in terms of wave arriving time and amplitude parameter). The corresponding software application exists today and is ready for use [1]. We consider the example of the coastal line of Japan. Numerical tests show that optimal installation of only 4 DART buoys (accounting the existing sea bed cable) will reduce the tsunami wave detection time to only 10 min after an underwater earthquake. Secondly, as was shown by this paper authors, the use of GPU/FPGA technologies accelerates the execution of the MOST (method of splitting tsunami) code by 100 times [2]. Therefore, tsunami wave propagation over the ocean area 2000*2000 km (wave propagation simulation: time step 10 sec, recording each 4th spatial point and 4th time step) could be calculated at: 3 sec with 4' mesh 50 sec with 1' mesh 5 min with 0.5' mesh The algorithm to switch from coarse mesh to the fine grain one is also available. Finally, we propose the new algorithm for tsunami source parameters determination by real-time processing the time series, obtained at DART. It is possible to approximate the measured time series by a linear combination of synthetic marigrams. Coefficients of such linear combination are calculated with the help of orthogonal decomposition. The algorithm is very fast and demonstrates good accuracy. Summing up, using the example of the coastal line of Japan, wave height evaluation will be available in 12-14 minutes after the earthquake even before the wave approaches the nearest shore point (usually, it takes places in about 20 minutes). The determination of the optimal sensors' location using genetic algorithm / A.S.Astrakova, D.V.Bannikov, S.G.Cherny, M.M.Lavrentiev // 3rd Nordic EMW Summer School, Turku, Finland, June, 2009: proceedings - Finland: TUSC General Publications, 2009. - N 53. - P.5-22. M.Lavrentiev Jr., A.Romanenko, "Modern Hardware Solutions to Speed Up Tsunami Simulation Codes", Geophysical research abstracts, Vol. 12, EGU2010-3835, 2010

Overview of the DART project

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berry, K.R.; Hansen, F.R.; Napolitano, L.M.

1992-01-01

DART (DSP Arrary for Reconfigurable Tasks) is a parallel architecture of two high-performance SDP (digital signal processing) chips with the flexibility to handle a wide range of real-time applications. Each of the 32-bit floating-point DSP processes in DART is programmable in a high-level languate ( C'' or Ada). We have added extensions to the real-time operating system used by DART in order to support parallel processor. The combination of high-level language programmability, a real-time operating system, and parallel processing support significantly reduces the development cost of application software for signal processing and control applications. We have demonstrated this capability bymore » using DART to reconstruct images in the prototype VIP (Video Imaging Projectile) groundstation.« less

Overview of the DART project

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berry, K.R.; Hansen, F.R.; Napolitano, L.M.

1992-01-01

DART (DSP Arrary for Reconfigurable Tasks) is a parallel architecture of two high-performance SDP (digital signal processing) chips with the flexibility to handle a wide range of real-time applications. Each of the 32-bit floating-point DSP processes in DART is programmable in a high-level languate (``C`` or Ada). We have added extensions to the real-time operating system used by DART in order to support parallel processor. The combination of high-level language programmability, a real-time operating system, and parallel processing support significantly reduces the development cost of application software for signal processing and control applications. We have demonstrated this capability by usingmore » DART to reconstruct images in the prototype VIP (Video Imaging Projectile) groundstation.« less

Quantitation of Mycotoxins Using Direct Analysis in Real Time Mass Spectrometry (DART-MS).

PubMed

Busman, Mark

2018-05-01

Ambient ionization represents a new generation of MS ion sources and is used for the rapid ionization of small molecules under ambient conditions. The combination of ambient ionization and MS allows the analysis of multiple food samples with simple or no sample treatment or in conjunction with prevailing sample preparation methods. Two ambient ionization methods, desorptive electrospray ionization (DESI) and direct analysis in real time (DART) have been adapted for food safety application. Both ionization techniques provide unique advantages and capabilities. DART has been used for a variety of qualitative and quantitative applications. In particular, mycotoxin contamination of food and feed materials has been addressed by DART-MS. Applications to mycotoxin analysis by ambient ionization MS and particularly DART-MS are summarized.

Genome-wide characterization of genetic diversity and population structure in Secale

PubMed Central

2014-01-01

Background Numerous rye accessions are stored in ex situ genebanks worldwide. Little is known about the extent of genetic diversity contained in any of them and its relation to contemporary varieties, since to date rye genetic diversity studies had a very limited scope, analyzing few loci and/ or few accessions. Development of high throughput genotyping methods for rye opened the possibility for genome wide characterizations of large accessions sets. In this study we used 1054 Diversity Array Technology (DArT) markers with defined chromosomal location to characterize genetic diversity and population structure in a collection of 379 rye accessions including wild species, landraces, cultivated materials, historical and contemporary rye varieties. Results Average genetic similarity (GS) coefficients and average polymorphic information content (PIC) values varied among chromosomes. Comparison of chromosome specific average GS within and between germplasm sub-groups indicated regions of chromosomes 1R and 4R as being targeted by selection in current breeding programs. Bayesian clustering, principal coordinate analysis and Neighbor Joining clustering demonstrated that source and improvement status contributed significantly to the structure observed in the analyzed set of Secale germplasm. We revealed a relatively limited diversity in improved rye accessions, both historical and contemporary, as well as lack of correlation between clustering of improved accessions and geographic origin, suggesting common genetic background of rye accessions from diverse geographic regions and extensive germplasm exchange. Moreover, contemporary varieties were distinct from the remaining accessions. Conclusions Our results point to an influence of reproduction methods on the observed diversity patterns and indicate potential of ex situ collections for broadening the genetic diversity in rye breeding programs. Obtained data show that DArT markers provide a realistic picture of the genetic diversity and population structure present in the collection of 379 rye accessions and are an effective platform for rye germplasm characterization and association mapping studies. PMID:25085433

Stepped-to-dart Leaders in Cloud-to-ground Lightning

NASA Astrophysics Data System (ADS)

Stolzenburg, M.; Marshall, T. C.; Karunarathne, S.; Karunarathna, N.; Warner, T.; Orville, R. E.

2013-12-01

Using time-correlated high-speed video (50,000 frames per second) and fast electric field change (5 MegaSamples per second) data for lightning flashes in East-central Florida, we describe an apparently rare type of subsequent leader: a stepped leader that finds and follows a previously used channel. The observed 'stepped-to-dart leaders' occur in three natural negative ground flashes. Stepped-to-dart leader connection altitudes are 3.3, 1.6 and 0.7 km above ground in the three cases. Prior to the stepped-to-dart connection, the advancing leaders have properties typical of stepped leaders. After the connection, the behavior changes almost immediately (within 40-60 us) to dart or dart-stepped leader, with larger amplitude E-change pulses and faster average propagation speeds. In this presentation, we will also describe the upward luminosity after the connection in the prior return stroke channel and in the stepped leader path, along with properties of the return strokes and other leaders in the three flashes.

DART: Delta Advanced Reusable Transport. An alternate manned space system proposal

NASA Technical Reports Server (NTRS)

1991-01-01

The Delta Advanced Reusable Transport (DART) craft is being developed to add, multiple, rapid, and cost effective space access to the U.S. capability and to further the efforts towards a permanent space presence. The DART craft provides an augmentative and an alternative system to the Shuttle. As a supplement launch vehicle, the DART adds low cost and easily accessible transport of crew and cargo to specific space destinations to the U.S. program. This adds significant opportunities for manned rated missions that do not require Shuttle capabilities. In its alternative role, the DART can provide emergency space access and satellite repair, the continuation of scientific research, and the furthering of U.S. manned efforts in the event of Shuttle incapabilities. In addition, the DART is being designed for Space Station Freedom compatibility, including its use as a 'lifeboat' emergency reentry craft for Freedom astronauts, as well as the transport of crew and cargo for station resupply.

In-vivo confirmation of the use of the dart thrower's motion during activities of daily living.

PubMed

Brigstocke, G H O; Hearnden, A; Holt, C; Whatling, G

2014-05-01

The dart thrower's motion is a wrist rotation along an oblique plane from radial extension to ulnar flexion. We report an in-vivo study to confirm the use of the dart thrower's motion during activities of daily living. Global wrist motion in ten volunteers was recorded using a three-dimensional optoelectronic motion capture system, in which digital infra-red cameras track the movement of retro-reflective marker clusters. Global wrist motion has been approximated to the dart thrower's motion when hammering a nail, throwing a ball, drinking from a glass, pouring from a jug and twisting the lid of a jar, but not when combing hair or manipulating buttons. The dart thrower's motion is the plane of global wrist motion used during most activities of daily living. Arthrodesis of the radiocarpal joint instead of the midcarpal joint will allow better wrist function during most activities of daily living by preserving the dart thrower's motion.

Clinical measurement of the dart throwing motion of the wrist: variability, accuracy and correction.

PubMed

Vardakastani, Vasiliki; Bell, Hannah; Mee, Sarah; Brigstocke, Gavin; Kedgley, Angela E

2018-01-01

Despite being functionally important, the dart throwing motion is difficult to assess accurately through goniometry. The objectives of this study were to describe a method for reliably quantifying the dart throwing motion using goniometric measurements within a healthy population. Wrist kinematics of 24 healthy participants were assessed using goniometry and optical motion tracking. Three wrist angles were measured at the starting and ending points of the motion: flexion-extension, radial-ulnar deviation and dart throwing motion angle. The orientation of the dart throwing motion plane relative to the flexion-extension axis ranged between 28° and 57° among the tested population. Plane orientations derived from optical motion capture differed from those calculated through goniometry by 25°. An equation to correct the estimation of the plane from goniometry measurements was derived. This was applied and differences in the orientation of the plane were reduced to non-significant levels, enabling the dart throwing motion to be measured using goniometry alone.

Source identification of western Oregon Douglas-fir wood cores using mass spectrometry and random forest classification1

PubMed Central

Finch, Kristen; Espinoza, Edgard; Jones, F. Andrew; Cronn, Richard

2017-01-01

Premise of the study: We investigated whether wood metabolite profiles from direct analysis in real time (time-of-flight) mass spectrometry (DART-TOFMS) could be used to determine the geographic origin of Douglas-fir wood cores originating from two regions in western Oregon, USA. Methods: Three annual ring mass spectra were obtained from 188 adult Douglas-fir trees, and these were analyzed using random forest models to determine whether samples could be classified to geographic origin, growth year, or growth year and geographic origin. Specific wood molecules that contributed to geographic discrimination were identified. Results: Douglas-fir mass spectra could be differentiated into two geographic classes with an accuracy between 70% and 76%. Classification models could not accurately classify sample mass spectra based on growth year. Thirty-two molecules were identified as key for classifying western Oregon Douglas-fir wood cores to geographic origin. Discussion: DART-TOFMS is capable of detecting minute but regionally informative differences in wood molecules over a small geographic scale, and these differences made it possible to predict the geographic origin of Douglas-fir wood with moderate accuracy. Studies involving DART-TOFMS, alone and in combination with other technologies, will be relevant for identifying the geographic origin of illegally harvested wood. PMID:28529831

Tailoring CD19xCD3-DART exposure enhances T-cells to eradication of B-cell neoplasms.

PubMed

Circosta, Paola; Elia, Angela Rita; Landra, Indira; Machiorlatti, Rodolfo; Todaro, Maria; Aliberti, Sabrina; Brusa, Davide; Deaglio, Silvia; Chiaretti, Sabina; Bruna, Riccardo; Gottardi, Daniela; Massaia, Massimo; Giacomo, Filomena Di; Guarini, Anna Rita; Foà, Robin; Kyriakides, Peter W; Bareja, Rohan; Elemento, Olivier; Chichili, Gurunadh R; Monteleone, Emanuele; Moore, Paul A; Johnson, Syd; Bonvini, Ezio; Cignetti, Alessandro; Inghirami, Giorgio

2018-01-01

Many patients with B-cell malignancies can be successfully treated, although tumor eradication is rarely achieved. T-cell-directed killing of tumor cells using engineered T-cells or bispecific antibodies is a promising approach for the treatment of hematologic malignancies. We investigated the efficacy of CD19xCD3 DART bispecific antibody in a broad panel of human primary B-cell malignancies. The CD19xCD3 DART identified 2 distinct subsets of patients, in which the neoplastic lymphocytes were eliminated with rapid or slow kinetics. Delayed responses were always overcome by a prolonged or repeated DART exposure. Both CD4 and CD8 effector cytotoxic cells were generated, and DART-mediated killing of CD4 + cells into cytotoxic effectors required the presence of CD8 + cells. Serial exposures to DART led to the exponential expansion of CD4 + and CD8 + cells and to the sequential ablation of neoplastic cells in absence of a PD-L1-mediated exhaustion. Lastly, patient-derived neoplastic B-cells (B-Acute Lymphoblast Leukemia and Diffuse Large B Cell Lymphoma) could be proficiently eradicated in a xenograft mouse model by DART-armed cytokine induced killer (CIK) cells. Collectively, patient tailored DART exposures can result in the effective elimination of CD19 positive leukemia and B-cell lymphoma and the association of bispecific antibodies with unmatched CIK cells represents an effective modality for the treatment of CD19 positive leukemia/lymphoma.

DART model for thermal conductivity of U{sub 3}Si{sub 2} aluminum dispersion fuel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rest, J.; Snelgrove, J.L.; Hofman, G.L.

1995-09-01

This paper describes the primary physical models that form the basis of the DART model for calculating irradiation-induced changes in the thermal conductivity of aluminium dispersion fuel. DART calculations of fuel swelling, pore closure, and thermal conductivity are compared with measured values.

Extending DART to meet the data acquisition needs of future experiments at Fermilab

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oleynik, G.; Pordes, R.; Barsotti, E.

1995-10-01

The DART project at Fermilab is a major collaboration to develop a data acquisition system for multiple experiments. The initial implementation of DART has concentrated on providing working data acquisition systems for the (now eight) collaborating experiments in the next Fixed Target Run. In this paper we discuss aspects of the architecture of DART and how these will allow it to be extended to meet the expected needs of future experiments at Fermilab. We also discuss some ongoing developments within the Fermilab Computing Division towards these new implementations.

FastDart : a fast, accurate and friendly version of DART code.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rest, J.; Taboada, H.

2000-11-08

A new enhanced, visual version of DART code is presented. DART is a mechanistic model based code, developed for the performance calculation and assessment of aluminum dispersion fuel. Major issues of this new version are the development of a new, time saving calculation routine, able to be run on PC, a friendly visual input interface and a plotting facility. This version, available for silicide and U-Mo fuels,adds to the classical accuracy of DART models for fuel performance prediction, a faster execution and visual interfaces. It is part of a collaboration agreement between ANL and CNEA in the area of Lowmore » Enriched Uranium Advanced Fuels, held by the Implementation Arrangement for Technical Exchange and Cooperation in the Area of Peaceful Uses of Nuclear Energy.« less

Detection of caffeine in tea, instant coffee, green tea beverage, and soft drink by direct analysis in real time (DART) source coupled to single-quadrupole mass spectrometry.

PubMed

Wang, Lei; Zhao, Pengyue; Zhang, Fengzu; Bai, Aijuan; Pan, Canping

2013-01-01

Ambient ionization direct analysis in real time (DART) coupled to single-quadrupole MS (DART-MS) was evaluated for rapid detection of caffeine in commercial samples without chromatographic separation or sample preparation. Four commercial samples were examined: tea, instant coffee, green tea beverage, and soft drink. The response-related parameters were optimized for the DART temperature and MS fragmentor. Under optimal conditions, the molecular ion (M+H)+ was the major ion for identification of caffeine. The results showed that DART-MS is a promising tool for the quick analysis of important marker molecules in commercial samples. Furthermore, this system has demonstrated significant potential for high sample throughput and real-time analysis.

3-D discrete analytical ridgelet transform.

PubMed

Helbert, David; Carré, Philippe; Andres, Eric

2006-12-01

In this paper, we propose an implementation of the 3-D Ridgelet transform: the 3-D discrete analytical Ridgelet transform (3-D DART). This transform uses the Fourier strategy for the computation of the associated 3-D discrete Radon transform. The innovative step is the definition of a discrete 3-D transform with the discrete analytical geometry theory by the construction of 3-D discrete analytical lines in the Fourier domain. We propose two types of 3-D discrete lines: 3-D discrete radial lines going through the origin defined from their orthogonal projections and 3-D planes covered with 2-D discrete line segments. These discrete analytical lines have a parameter called arithmetical thickness, allowing us to define a 3-D DART adapted to a specific application. Indeed, the 3-D DART representation is not orthogonal, It is associated with a flexible redundancy factor. The 3-D DART has a very simple forward/inverse algorithm that provides an exact reconstruction without any iterative method. In order to illustrate the potentiality of this new discrete transform, we apply the 3-D DART and its extension to the Local-DART (with smooth windowing) to the denoising of 3-D image and color video. These experimental results show that the simple thresholding of the 3-D DART coefficients is efficient.

TVR-DART: A More Robust Algorithm for Discrete Tomography From Limited Projection Data With Automated Gray Value Estimation.

PubMed

Xiaodong Zhuge; Palenstijn, Willem Jan; Batenburg, Kees Joost

2016-01-01

In this paper, we present a novel iterative reconstruction algorithm for discrete tomography (DT) named total variation regularized discrete algebraic reconstruction technique (TVR-DART) with automated gray value estimation. This algorithm is more robust and automated than the original DART algorithm, and is aimed at imaging of objects consisting of only a few different material compositions, each corresponding to a different gray value in the reconstruction. By exploiting two types of prior knowledge of the scanned object simultaneously, TVR-DART solves the discrete reconstruction problem within an optimization framework inspired by compressive sensing to steer the current reconstruction toward a solution with the specified number of discrete gray values. The gray values and the thresholds are estimated as the reconstruction improves through iterations. Extensive experiments from simulated data, experimental μCT, and electron tomography data sets show that TVR-DART is capable of providing more accurate reconstruction than existing algorithms under noisy conditions from a small number of projection images and/or from a small angular range. Furthermore, the new algorithm requires less effort on parameter tuning compared with the original DART algorithm. With TVR-DART, we aim to provide the tomography society with an easy-to-use and robust algorithm for DT.

Quantitative analysis of major dibenzocyclooctane lignans in Schisandrae fructus by online TLC-DART-MS.

PubMed

Kim, Hye Jin; Oh, Myung Sook; Hong, Jongki; Jang, Young Pyo

2011-01-01

Direct analysis in real time (DART) ion source is a powerful ionising technique for the quick and easy detection of various organic molecules without any sample preparation steps, but the lack of quantitation capacity limits its extensive use in the field of phytochemical analysis. To improvise a new system which utilize DART-MS as a hyphenated detector for quantitation. A total extract of Schisandra chinensis fruit was analyzed on a TLC plate and three major lignan compounds were quantitated by three different methods of UV densitometry, TLC-DART-MS and HPLC-UV to compare the efficiency of each method. To introduce the TLC plate into the DART ion source at a constant velocity, a syringe pump was employed. The DART-MS total ion current chromatogram was recorded for the entire TLC plate. The concentration of each lignan compound was calculated from the calibration curve established with standard compound. Gomisin A, gomisin N and schisandrin were well separated on a silica-coated TLC plate and the specific ion current chromatograms were successfully acquired from the TLC-DART-MS system. The TLC-DART-MS system for the quantitation of natural products showed better linearity and specificity than TLC densitometry, and consumed less time and solvent than conventional HPLC method. A hyphenated system for the quantitation of phytochemicals from crude herbal drugs was successfully established. This system was shown to have a powerful analytical capacity for the prompt and efficient quantitation of natural products from crude drugs. Copyright © 2010 John Wiley & Sons, Ltd.

DART: A Community Facility Providing State-of-the-Art, Efficient Ensemble Data Assimilation for Large (Coupled) Geophysical Models

NASA Astrophysics Data System (ADS)

Hoar, T. J.; Anderson, J. L.; Collins, N.; Kershaw, H.; Hendricks, J.; Raeder, K.; Mizzi, A. P.; Barré, J.; Gaubert, B.; Madaus, L. E.; Aydogdu, A.; Raeder, J.; Arango, H.; Moore, A. M.; Edwards, C. A.; Curchitser, E. N.; Escudier, R.; Dussin, R.; Bitz, C. M.; Zhang, Y. F.; Shrestha, P.; Rosolem, R.; Rahman, M.

2016-12-01

Strongly-coupled ensemble data assimilation with multiple high-resolution model components requires massive state vectors which need to be efficiently stored and accessed throughout the assimilation process. Supercomputer architectures are tending towards increasing the number of cores per node but have the same or less memory per node. Recent advances in the Data Assimilation Research Testbed (DART), a freely-available community ensemble data assimilation facility that works with dozens of large geophysical models, have addressed the need to run with a smaller memory footprint on a higher node count by utilizing MPI-2 one-sided communication to do non-blocking asynchronous access of distributed data. DART runs efficiently on many computational platforms ranging from laptops through thousands of cores on the newest supercomputers. Benefits of the new DART implementation will be shown. In addition, overviews of the most recently supported models will be presented: CAM-CHEM, WRF-CHEM, CM1, OpenGGCM, FESOM, ROMS, CICE5, TerrSysMP (COSMO, CLM, ParFlow), JULES, and CABLE. DART provides a comprehensive suite of software, documentation, and tutorials that can be used for ensemble data assimilation research, operations, and education. Scientists and software engineers at NCAR are available to support DART users who want to use existing DART products or develop their own applications. Current DART users range from university professors teaching data assimilation, to individual graduate students working with simple models, through national laboratories and state agencies doing operational prediction with large state-of-the-art models.

DART: A Microcomputer Program for Response Latency Analysis.

ERIC Educational Resources Information Center

Greene, John O.; Greene, Barry F.

1987-01-01

Discusses how chronometric measures such as the DART (Display And Response Timing) computer program, have become virtually indispensable in testing cognitive theories of human social behavior. Describes how the DART (1) provides a way to collect response latency data; and (2) allows measurement of response latencies to a set of user-specified,…

Reuse, Repurposing and Learning Design--Lessons from the DART Project

ERIC Educational Resources Information Center

Bond, Stephen T.; Ingram, Caroline; Ryan, Steve

2008-01-01

Digital Anthropological Resources for Teaching (DART) is a major project examining ways in which the use of online learning activities and repositories can enhance the teaching of anthropology and, by extension, other disciplines. This paper reports on one strand of DART activity, the development of customisable learning activities that can be…

75 FR 31820 - Notice of Applications for Deregistration Under Section 8(f) of the Investment Company Act of 1940

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-04

.... Each applicant also distributed preferred shares of Dutch Auction Rate Transferable Securities (``DARTS'') of the acquiring fund to holders of applicants' Auction Rate Preferred Shares, DARTS, or Auction... distributed Dutch Auction Rate Transferable Securities (``DARTS'') of the acquiring fund to the holders of...

KSC-04pd1684

NASA Image and Video Library

2004-07-16

KENNEDY SPACE CENTER, FLA. - An artist’s conception of the autonomous Demonstration for Autonomous Rendezvous (DART) spacecraft as it approaches the Multiple Paths, Beyond-Line-of-Site Communications (MUBLCOM) satellite. NASA is testing the DART as a docking system for next generation vehicles to guide spacecraft carrying cargo or equipment to the International Space Station, or retrieving or servicing satellites in orbit. Before the new system can be implemented on piloted spacecraft, it has to be tested in space. The computer-guided DART is equipped with an Advanced Video Guidance Sensor and a Global Positioning System that can receive signals from other spacecraft to allow DART to move within 330 feet of the target. DART is scheduled to launch from Vandenberg Air Force Base in California no earlier than Oct. 18. It will be released from a Pegasus XL launch vehicle carried aloft by an Orbital Sciences Corporation aircraft. The fourth stage of the Pegasus rocket will remain attached as an integral part of the spacecraft, allowing it to maneuver in space. Once in orbit, DART will race toward the target, the MUBLCOM satellite, for a rendezvous.

KSC-04pd1686

NASA Image and Video Library

2004-07-16

KENNEDY SPACE CENTER, FLA. - An artist’s conception of the autonomous Demonstration for Autonomous Rendezvous (DART) spacecraft as it approaches the Multiple Paths, Beyond-Line-of-Site Communications (MUBLCOM) satellite. NASA is testing the DART as a docking system for next generation vehicles to guide spacecraft carrying cargo or equipment to the International Space Station, or retrieving or servicing satellites in orbit. Before the new system can be implemented on piloted spacecraft, it has to be tested in space. The computer-guided DART is equipped with an Advanced Video Guidance Sensor and a Global Positioning System that can receive signals from other spacecraft to allow DART to move within 330 feet of the target. DART is scheduled to launch from Vandenberg Air Force Base in California no earlier than Oct. 18. It will be released from a Pegasus XL launch vehicle carried aloft by an Orbital Sciences Corporation aircraft. The fourth stage of the Pegasus rocket will remain attached as an integral part of the spacecraft, allowing it to maneuver in space. Once in orbit, DART will race toward the target, the MUBLCOM satellite, for a rendezvous.

KSC-04pd1685

NASA Image and Video Library

2004-07-16

KENNEDY SPACE CENTER, FLA. - An artist’s conception of the autonomous Demonstration for Autonomous Rendezvous (DART) spacecraft as it approaches the Multiple Paths, Beyond-Line-of-Site Communications (MUBLCOM) satellite. NASA is testing the DART as a docking system for next generation vehicles to guide spacecraft carrying cargo or equipment to the International Space Station, or retrieving or servicing satellites in orbit. Before the new system can be implemented on piloted spacecraft, it has to be tested in space. The computer-guided DART is equipped with an Advanced Video Guidance Sensor and a Global Positioning System that can receive signals from other spacecraft to allow DART to move within 330 feet of the target. DART is scheduled to launch from Vandenberg Air Force Base in California no earlier than Oct. 18. It will be released from a Pegasus XL launch vehicle carried aloft by an Orbital Sciences Corporation aircraft. The fourth stage of the Pegasus rocket will remain attached as an integral part of the spacecraft, allowing it to maneuver in space. Once in orbit, DART will race toward the target, the MUBLCOM satellite, for a rendezvous.

Smart darting diffusion Monte Carlo: Applications to lithium ion-Stockmayer clusters

NASA Astrophysics Data System (ADS)

Christensen, H. M.; Jake, L. C.; Curotto, E.

2016-05-01

In a recent investigation [K. Roberts et al., J. Chem. Phys. 136, 074104 (2012)], we have shown that, for a sufficiently complex potential, the Diffusion Monte Carlo (DMC) random walk can become quasiergodic, and we have introduced smart darting-like moves to improve the sampling. In this article, we systematically characterize the bias that smart darting moves introduce in the estimate of the ground state energy of a bosonic system. We then test a simple approach to eliminate completely such bias from the results. The approach is applied for the determination of the ground state of lithium ion-n-dipoles clusters in the n = 8-20 range. For these, the smart darting diffusion Monte Carlo simulations find the same ground state energy and mixed-distribution as the traditional approach for n < 14. In larger systems we find that while the ground state energies agree quantitatively with or without smart darting moves, the mixed-distributions can be significantly different. Some evidence is offered to conclude that introducing smart darting-like moves in traditional DMC simulations may produce a more reliable ground state mixed-distribution.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Enders, Alexander L.; Lousteau, Angela L.

The Desktop Analysis Reporting Tool (DART) is a software package that allows users to easily view and analyze daily files that span long periods. DART gives users the capability to quickly determine the state of health of a radiation portal monitor (RPM), troubleshoot and diagnose problems, and view data in various time frames to perform trend analysis. In short, it converts the data strings written in the daily files into meaningful tables and plots. The standalone version of DART (“soloDART”) utilizes a database engine that is included with the application; no additional installations are necessary. There is also a networkedmore » version of DART (“polyDART”) that is designed to maximize the benefit of a centralized data repository while distributing the workload to individual desktop machines. This networked approach requires a more complex database manager Structured Query Language (SQL) Server; however, SQL Server is not currently provided with DART. Regardless of which version is used, DART will import daily files from RPMs, store the relevant data in its database, and it can produce reports for status, trend analysis, and reporting purposes.« less

Applications of DART-MS for food quality and safety assurance in food supply chain.

PubMed

Guo, Tianyang; Yong, Wei; Jin, Yong; Zhang, Liya; Liu, Jiahui; Wang, Sai; Chen, Qilong; Dong, Yiyang; Su, Haijia; Tan, Tianwei

2017-03-01

Direct analysis in real time (DART) represents a new generation of ion source which is used for rapid ionization of small molecules under ambient conditions. The combination of DART and various mass spectrometers allows analyzing multiple food samples with simple or no sample treatment, or in conjunction with prevailing protocolized sample preparation methods. Abundant applications by DART-MS have been reviewed in this paper. The DART-MS strategy applied to food supply chain (FSC), including production, processing, and storage and transportation, provides a comprehensive solution to various food components, contaminants, authenticity, and traceability. Additionally, typical applications available in food analysis by other ambient ionization mass spectrometers were summarized, and fundamentals mainly including mechanisms, devices, and parameters were discussed as well. © 2015 Wiley Periodicals, Inc. Mass Spec Rev. 36:161-187, 2017. © 2015 Wiley Periodicals, Inc.

Hyperthyroidism with dome-and-dart T wave: A case report: A care-compliant article.

PubMed

Lai, Ping; Yuan, Jing-Ling; Xue, Jin-Hua; Qiu, Yue-Qun

2017-02-01

Dome-and-dart T waves (or bifid T waves) are a rare phenomenon in the surface electrocardiogram. These wave forms are mainly observed in patients with congenital heart disease such as atrial septal defect and ventricular septal defect. And hyperthyroidism who presented with an electrocardiogram that had dome-and-dart T waves in a precordial lead is never been reported. The patient presented with continuous tachycardia, palpitations, chest tightness, and headache for 4 days, and aggravated for 1 day. Hyperthyroidism. Methimazole. All symptoms were alleviated. Dome-and-dart or bifid T waves have been reported in the conventional 12-lead electrocardiograms in some patients with congenital heart disease. The case illustrated here, to the best of our knowledge, dome-and-dart or bifid T waves may associate with hyperthyroidism patients.

Hyperthyroidism with dome-and-dart T wave: A case report

PubMed Central

Lai, Ping; Yuan, Jing-ling; Xue, Jin-hua; Qiu, Yue-qun

2017-01-01

Abstract Rationale: Dome-and-dart T waves (or bifid T waves) are a rare phenomenon in the surface electrocardiogram. These wave forms are mainly observed in patients with congenital heart disease such as atrial septal defect and ventricular septal defect. And hyperthyroidism who presented with an electrocardiogram that had dome-and-dart T waves in a precordial lead is never been reported. Patient concerns: The patient presented with continuous tachycardia, palpitations, chest tightness, and headache for 4 days, and aggravated for 1 day. Diagnoses: Hyperthyroidism. Interventions: Methimazole. Outcomes: All symptoms were alleviated. Lessons: Dome-and-dart or bifid T waves have been reported in the conventional 12-lead electrocardiograms in some patients with congenital heart disease. The case illustrated here, to the best of our knowledge, dome-and-dart or bifid T waves may associate with hyperthyroidism patients. PMID:28178156

B chromosomes are associated with redistribution of genetic recombination towards lower recombination chromosomal regions in perennial ryegrass.

PubMed

Harper, John; Phillips, Dylan; Thomas, Ann; Gasior, Dagmara; Evans, Caron; Powell, Wayne; King, Julie; King, Ian; Jenkins, Glyn; Armstead, Ian

2018-04-09

Supernumerary 'B' chromosomes are non-essential components of the genome present in a range of plant and animal species-including many grasses. Within diploid and polyploid ryegrass and fescue species, including the forage grass perennial ryegrass (Lolium perenne L.), the presence of B chromosomes has been reported as influencing both chromosome pairing and chiasma frequencies. In this study, the effects of the presence/absence of B chromosomes on genetic recombination has been investigated through generating DArT (Diversity Arrays Technology) marker genetic maps for six perennial ryegrass diploid populations, the pollen parents of which contained either two B or zero B chromosomes. Through genetic and cytological analyses of these progeny and their parents, we have identified that, while overall cytological estimates of chiasma frequencies were significantly lower in pollen mother cells with two B chromosomes as compared with zero B chromosomes, the recombination frequencies within some marker intervals were actually increased, particularly for marker intervals in lower recombination regions of chromosomes, namely pericentromeric regions. Thus, in perennial ryegrass, the presence of two B chromosomes redistributed patterns of meiotic recombination in pollen mother cells in ways which could increase the range of allelic variation available to plant breeders.

Integrating mental health into adolescent annual visits: impact of previsit comprehensive screening on within-visit processes.

PubMed

Gadomski, Anne M; Fothergill, Kate E; Larson, Susan; Wissow, Lawrence S; Winegrad, Heather; Nagykaldi, Zsolt J; Olson, Ardis L; Roter, Debra L

2015-03-01

To evaluate how a comprehensive, computerized, self-administered adolescent screener, the DartScreen, affects within-visit patient-doctor interactions such as data gathering, advice giving, counseling, and discussion of mental health issues. Patient-doctor interaction was compared between visits without screening and those with the DartScreen completed before the visit. Teens, aged 15-19 years scheduled for an annual visit, were recruited at one urban and one rural pediatric primary care clinic. The doctor acted as his/her own control, first using his/her usual routine for five to six adolescent annual visits. Then, the DartScreen was introduced for five visits where at the beginning of the visit, the doctor received a summary report of the screening results. All visits were audio recorded and analyzed using the Roter interaction analysis system. Doctor and teen dialogue and topics discussed were compared between the two groups. Seven midcareer doctors and 72 adolescents participated; 37 visits without DartScreen and 35 with DartScreen were audio recorded. The Roter interaction analysis system defined medically related data gathering (mean, 36.8 vs. 32.7 statements; p = .03) and counseling (mean, 36.8 vs. 32.7 statements; p = .01) decreased with DartScreen; however, doctor responsiveness and engagement improved with DartScreen (mean, 4.8 vs. 5.1 statements; p = .00). Teens completing the DartScreen offered more psychosocial information (mean, 18.5 vs. 10.6 statements; p = .01), and mental health was discussed more after the DartScreen (mean, 93.7 vs. 43.5 statements; p = .03). Discussion of somatic and substance abuse topics did not change. Doctors reported that screening improved visit organization and efficiency. Use of the screener increased discussion of mental health but not at the expense of other adolescent health topics. Copyright © 2015 Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.

Comparison of strain rates of dart impacted plaques and pendulum impacted bumpers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Scammell, K.L.

1987-01-01

The difference in strain rates prevailing during pendulum impact of bumpers versus high speed dart impact of plaques was investigated. Uni-axial strain gages were applied to the tension side of the plaques and bumpers directly opposite the point of impact. The plaques were impacted with an instrumented high rate dart impact tester and the bumpers impacted with a full scale bumper pendulum impact tester. Theoretical calculations and actual strain rate data support the conclusion that the strain rate of a plaque during dart impact significantly exceeds that of bumper strain rate during pendulum impact.

AIDA DART asteroid deflection test: Planetary defense and science objectives

NASA Astrophysics Data System (ADS)

Cheng, Andrew F.; Rivkin, Andrew S.; Michel, Patrick; Atchison, Justin; Barnouin, Olivier; Benner, Lance; Chabot, Nancy L.; Ernst, Carolyn; Fahnestock, Eugene G.; Kueppers, Michael; Pravec, Petr; Rainey, Emma; Richardson, Derek C.; Stickle, Angela M.; Thomas, Cristina

2018-08-01

The Asteroid Impact & Deflection Assessment (AIDA) mission is an international cooperation between NASA and ESA. NASA plans to provide the Double Asteroid Redirection Test (DART) mission which will perform a kinetic impactor experiment to demonstrate asteroid impact hazard mitigation. ESA proposes to provide the Hera mission which will rendezvous with the target to monitor the deflection, perform detailed characterizations, and measure the DART impact outcomes and momentum transfer efficiency. The primary goals of AIDA are (i) to demonstrate the kinetic impact technique on a potentially hazardous near-Earth asteroid and (ii) to measure and characterize the deflection caused by the impact. The AIDA target will be the binary asteroid (65803) Didymos, which is of spectral type Sq, with the deflection experiment to occur in October, 2022. The DART impact on the secondary member of the binary at ∼6 km/s changes the orbital speed and the binary orbit period, which can be measured by Earth-based observatories with telescope apertures as small as 1 m. The DART impact will in addition alter the orbital and rotational states of the Didymos binary, leading to excitation of eccentricity and libration that, if measured by Hera, can constrain internal structure of the target asteroid. Measurements of the DART crater diameter and morphology can constrain target properties like cohesion and porosity based on numerical simulations of the DART impact.

Efficacy of immobilizing free-ranging elk with Telazol® and xylazine hydrochloride using transmitter-equipped darts

USGS Publications Warehouse

Walter, W. David; Leslie, David M.; Herner-Thogmartin, Jennifer H.; Smith, Kimberly G.; Cartwright, Michael E.

2005-01-01

From January 1999 to April 2002, 14 free-ranging elk were darted with a mixture of Telazol® reconstituted with xylazine hydrochloride (HCl) in a forested habitat in southwestern Oklahoma and north-central Arkansas. Elk were darted from ground blinds, tree stands, or a vehicle at distances of 14–46 m and were recovered 37–274 m from the dart site. Elk were located using radiotelemetry with 3-cc disposable Pneu-dart® transmitter darts. Mean±SD dose of Telazol®and xylazine HCl was 590±192 mg/ml and 276±153 mg/ml, respectively, and mean time to standing after injection of reversal agent was 27 min (range: 1–65 min). The combination of Telazol® and xylazine HCl successfully immobilized free-ranging elk, and transmitter-equipped darts permitted successful location of sedated elk by two people in areas of dense forest cover. The dose required to sedate elk appeared to vary depending on physiology and behavior, but no drug-induced mortality occurred despite the wide variance in the doses administered. We recommend 500 mg Telazol® reconstituted with 300 mg xylazine HCl as an initial dose for a ≥200 kg elk. If needed to achieve full sedation, up to 3 additional ml of the mixture may be administered without adverse effects.

16 CFR 1306.4 - Findings.

Code of Federal Regulations, 2012 CFR

2012-01-01

... are intended to be used outdoors and that are designed so that when they are thrown into the air they will contact the ground tip first. Often, lawn darts are used in a game where the darts are thrown at a..., nor is it intended to apply to indoor dart games that use a vertically-placed target, such as “English...

16 CFR 1306.4 - Findings.

Code of Federal Regulations, 2014 CFR

2014-01-01

... are intended to be used outdoors and that are designed so that when they are thrown into the air they will contact the ground tip first. Often, lawn darts are used in a game where the darts are thrown at a..., nor is it intended to apply to indoor dart games that use a vertically-placed target, such as “English...

16 CFR 1306.4 - Findings.

Code of Federal Regulations, 2011 CFR

2011-01-01

... are intended to be used outdoors and that are designed so that when they are thrown into the air they will contact the ground tip first. Often, lawn darts are used in a game where the darts are thrown at a..., nor is it intended to apply to indoor dart games that use a vertically-placed target, such as “English...

16 CFR § 1306.4 - Findings.

Code of Federal Regulations, 2013 CFR

2013-01-01

... elongated tips that are intended to be used outdoors and that are designed so that when they are thrown into the air they will contact the ground tip first. Often, lawn darts are used in a game where the darts... arrows or horseshoes, nor is it intended to apply to indoor dart games that use a vertically-placed...

75 FR 39303 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-08

.... DART. DART is a routing option currently offered by the Exchange in which the entering firm instructs the System to first route to alternative trading systems included in the System routing table. DART... . Please include File No. SR-BATS-2010-017 on the subject line. Paper Comments Send paper comments in...

The DART System for Far-IR/Submillimeter Space Missions

NASA Technical Reports Server (NTRS)

Dragovan, Mark

2004-01-01

The DART is a system of two cylindrical-parabolic reflectors. One reflector will produce a line focus; two reflectors properly oriented will produce a point focus. For far-infrared/submillimeter missions, the DART presents a compelling new telescope architecture that is scalable to alrge apertures, and with it's large membrane area is well suited to passive cooling.

The Dart Mentor Teacher Model: Training Early Childhood Supervisors To Assist Beginning Teachers.

ERIC Educational Resources Information Center

Restaino-Kelly, Arlene; Handler, June Moss

DART is a New Jersey statewide center for Dissemination, Advocacy, Research, and Training related to infants and toddlers, their families, and caregivers. This article describes the Trainer of Trainers mentoring model adopted by DART to increase the competency of child care supervisors and therefore the quality of care for infants and toddlers. It…

Efficacy of dart or booster vaccination with strain RB51 in protecting bison against experimental Brucella abortus challenge

USDA-ARS?s Scientific Manuscript database

Vaccination is an effective tool for reducing the prevalence of brucellosis in natural hosts. In this study, we characterized the efficacy of the Brucella abortus strain RB51 (RB51) vaccine in bison when delivered by single intramuscular vaccination (Hand RB51), single pneumatic dart delivery (Dart ...

Hitting the Bull's-Eye: A Dart Game Simulation Using Graphing Calculator Technology

ERIC Educational Resources Information Center

Mittag, Kathleen Cage; Taylor, Sharon E.

2006-01-01

One problem that students have with mathematics is that they often view the topic as a series of unrelated ideas. Sometimes they are aware that they have to know one concept to move to the next, but what is done in geometry is not necessarily related to anything in algebra. This failure to recognize mathematical connections limits students'…

The Catalog of Event Data of the Operational Deep-ocean Assessment and Reporting of Tsunamis (DART) Stations at the National Data Buoy Center

NASA Astrophysics Data System (ADS)

Bouchard, R.; Locke, L.; Hansen, W.; Collins, S.; McArthur, S.

2007-12-01

DART systems are a critical component of the tsunami warning system as they provide the only real-time, in situ, tsunami detection before landfall. DART systems consist of a surface buoy that serves as a position locater and communications transceiver and a Bottom Pressure Recorder (BPR) on the seafloor. The BPR records temperature and pressure at 15-second intervals to a memory card for later retrieval for analysis and use by tsunami researchers, but the BPRs are normally recovered only once every two years. The DART systems also transmit subsets of the data, converted to an estimation of the sea surface height, in near real-time for use by the tsunami warning community. These data are available on NDBC's webpages, http://www.ndbc.noaa.gov/dart.shtml. Although not of the resolution of the data recorded to the BPR memory card, the near real-time data have proven to be of value in research applications [1]. Of particular interest are the DART data associated with geophysical events. The DART BPR continuously compares the measured sea height with a predicted sea-height and when the difference exceeds a threshold value, the BPR goes into Event Mode. Event Mode provides an extended, more frequent near real-time reporting of the sea surface heights for tsunami detection. The BPR can go into Event Mode because of geophysical triggers, such as tsunamis or seismic activity, which may or may not be tsunamigenic. The BPR can also go into Event Mode during recovery of the BPR as it leaves the seafloor, or when manually triggered by the Tsunami Warning Centers in advance of an expected tsunami. On occasion, the BPR will go into Event Mode without any associated tsunami or seismic activity or human intervention and these are considered "False'' Events. Approximately one- third of all Events can be classified as "False". NDBC is responsible for the operations, maintenance, and data management of the DART stations. Each DART station has a webpage with a drop-down list of all Events. NDBC maintains the non-geophysical Events in order to maintain the continuity of the time series records. In 2007, NDBC compiled all DART Events that occurred while under NDBC's operational control and made an assessment on their validity. The NDBC analysts performed the assessment using the characteristics of the data time series, triggering criteria, and associated seismic events. The compilation and assessments are catalogued in a NDBC technical document. The Catalog also includes a listing of the one-hour, high-resolution data, retrieved remotely from the BPRs that are not available on the web pages. The Events are classified by their triggering mechanism and listed by station location and, for those Events associated with geophysical triggers, they are listed by their associated seismic events. The Catalog provides researchers with a valuable tool in locating, assessing, and applying near real-time DART data to tsunami research and will be updated following DART Events. A link to the published Catalog can be found on the NDBC DART website, http://www.ndbc.noaa.gov/dart.shtml. Reference: [1] Gower, J. and F. González (2006), U.S. Warning System Detected the Sumatra Tsunami, Eos Trans. AGU, 87(10), 105-112.

The Double Asteroid Redirection Test in the AIDA Project

NASA Astrophysics Data System (ADS)

Cheng, Andrew; Rivkin, Andrew; Michel, Patrick

2016-04-01

The Asteroid Impact & Deflection Assessment (AIDA) mission will be the first space experiment to demonstrate asteroid impact hazard mitigation by using a kinetic impactor. AIDA is a joint ESA-NASA cooperative project, that includes the ESA Asteroid Impact Mission (AIM) rendezvous mission and the NASA Double Asteroid Redirection Test (DART) mission. The AIDA target is the near-Earth binary asteroid 65803 Didymos, which will make an unusually close approach to Earth in October, 2022. The ~300-kg DART spacecraft is designed to impact the Didymos secondary at 7 km/s and demonstrate the ability to modify its trajectory through momentum transfer. DART and AIM are currently Phase A studies supported by NASA and ESA respectively. The primary goals of AIDA are (1) perform a full-scale demonstration of the spacecraft kinetic impact technique for deflection of an asteroid, by targeting an object larger than ~100 m and large enough to qualify as a Potentially Hazardous Asteroid; (2) measure the resulting asteroid deflection, by targeting the secondary member of a binary NEO and measuring the period change of the binary orbit; (3) understand the hyper-velocity collision effects on an asteroid, including the long-term dynamics of impact ejecta; and validate models for momentum transfer in asteroid impacts, based on measured physical properties of the asteroid surface and sub-surface. The primary DART objectives are to demonstrate a hyper-velocity impact on the Didymos moon and to determine the resulting deflection from ground-based observatories. The DART impact on the Didymos secondary will cause a measurable change in the orbital period of the binary. Supporting Earth-based optical and radar observations and numerical simulation studies are an integral part of the DART mission. The baseline DART mission launches in December, 2020 to impact the Didymos secondary in September, 2022. There are multiple launch opportunities for DART leading to impact around the 2022 Didymos close approach to Earth. The AIM spacecraft will be launched in Dec. 2020 and arrive at Didymos in spring, 2022, several months before the DART impact. AIM will characterize the Didymos binary system by means of remote sensing and in-situ instruments both before and after the DART impact. The asteroid deflection will be measured to higher accuracy, and additional results of the DART impact, like the impact crater, will be studied in great detail by the AIM mission. The combined DART and AIM missions will provide the first measurements of momentum transfer efficiency β from hyper-velocity kinetic impact at full scale on an asteroid, where the impact conditions of the projectile are known, and physical properties and internal structures of the target asteroid are also characterized. The DART impact on the Didymos secondary is predicted to cause a ~4.4 minute change in the binary orbit period, assuming β=1, and is expected to be observable within a few days. The predicted β would be in the range 1.1 to 1.3 for a porous target material based on a variety of numerical and analytical methods, but may be much larger if the target is non-porous. The DART kinetic impact is predicted to make a crater of ~6 to ~17 meters diameter, depending on target physical properties, but will also release a large volume of particulate ejecta that may be directly observable from Earth or even resolvable as a coma or an ejecta tail by ground-based telescopes.

Desorption atmospheric pressure photoionization and direct analysis in real time coupled with travelling wave ion mobility mass spectrometry.

PubMed

Räsänen, Riikka-Marjaana; Dwivedi, Prabha; Fernández, Facundo M; Kauppila, Tiina J

2014-11-15

Ambient mass spectrometry (MS) is a tool for screening analytes directly from sample surfaces. However, background impurities may complicate the spectra and therefore fast separation techniques are needed. Here, we demonstrate the use of travelling wave ion mobility spectrometry in a comparative study of two ambient MS techniques. Desorption atmospheric pressure photoionization (DAPPI) and direct analysis in real time (DART) were coupled with travelling wave ion mobility mass spectrometry (TWIM-MS) for highly selective surface analysis. The ionization efficiencies of DAPPI and DART were compared. Test compounds were: bisphenol A, benzo[a]pyrene, ranitidine, cortisol and α-tocopherol. DAPPI-MS and DART-TWIM-MS were also applied to the analysis of chloroquine from dried blood spots, and α-tocopherol from almond surface, and DAPPI-TWIM-MS was applied to analysis of pharmaceuticals and multivitamin tablets. DAPPI was approximately 100 times more sensitive than DART for bisphenol A and 10-20 times more sensitive for the other compounds. The limits of detection were between 30-290 and 330-8200 fmol for DAPPI and DART, respectively. Also, from the authentic samples, DAPPI ionized chloroquine and α-tocopherol more efficiently than DART. The mobility separation enabled the detection of species with low signal intensities, e.g. thiamine and cholecalciferol, in the DAPPI-TWIM-MS analysis of multivitamin tablets. DAPPI ionized the studied compounds of interest more efficiently than DART. For both DAPPI and DART, the mobility separation prior to MS analysis reduced the amount of chemical noise in the mass spectrum and significantly increased the signal-to-noise ratio for the analytes. Copyright © 2014 John Wiley & Sons, Ltd.

Sm protein methylation is dispensable for snRNP assembly in Drosophila melanogaster.

PubMed

Gonsalvez, Graydon B; Praveen, Kavita; Hicks, Amanda J; Tian, Liping; Matera, A Gregory

2008-05-01

Sm proteins form stable ribonucleoprotein (RNP) complexes with small nuclear (sn)RNAs and are core components of the eukaryotic spliceosome. In vivo, the assembly of Sm proteins onto snRNAs requires the survival motor neurons (SMN) complex. Several reports have shown that SMN protein binds with high affinity to symmetric dimethylarginine (sDMA) residues present on the C-terminal tails of SmB, SmD1, and SmD3. This post-translational modification is thought to play a crucial role in snRNP assembly. In human cells, two distinct protein arginine methyltransferases (PRMT5 and PRMT7) are required for snRNP biogenesis. However, in Drosophila, loss of Dart5 (the fruit fly PRMT5 ortholog) has little effect on snRNP assembly, and homozygous mutants are completely viable. To resolve these apparent differences, we examined this topic in detail and found that Drosophila Sm proteins are also methylated by two methyltransferases, Dart5/PRMT5 and Dart7/PRMT7. Unlike dart5, we found that dart7 is an essential gene. However, the lethality associated with loss of Dart7 protein is apparently unrelated to defects in snRNP assembly. To conclusively test the requirement for sDMA modification of Sm proteins in Drosophila snRNP assembly, we constructed a fly strain that exclusively expresses an isoform of SmD1 that cannot be sDMA modified. Interestingly, these flies were viable, and snRNP assays revealed no defects in comparison to wild type. In contrast, dart5 mutants displayed a strong synthetic lethal phenotype in the presence of a hypomorphic Smn mutation. We therefore conclude that dart5 is required for viability when SMN is limiting.

Drosophila arginine methyltransferase 1 (DART1) is an ecdysone receptor co-repressor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kimura, Shuhei; Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574; Sawatsubashi, Shun

2008-07-11

Histone arginine methylation is an epigenetic marker that regulates gene expression by defining the chromatin state. Arginine methyltransferases, therefore, serve as transcriptional co-regulators. However, unlike other transcriptional co-regulators, the physiological roles of arginine methyltransferases are poorly understood. Drosophila arginine methyltransferase 1 (DART1), the mammalian PRMT1 homologue, methylates the arginine residue of histone H4 (H4R3me2). Disruption of DART1 in Drosophila by imprecise P-element excision resulted in low viability during metamorphosis in the pupal stages. In the pupal stage, an ecdysone hormone signal is critical for developmental progression. DART1 interacted with the nuclear ecdysone receptor (EcR) in a ligand-dependent manner, and co-repressedmore » EcR in intact flies. These findings suggest that DART1, a histone arginine methyltransferase, is a co-repressor of EcR that is indispensable for normal pupal development in the intact fly.« less

DART: a practical reconstruction algorithm for discrete tomography.

PubMed

Batenburg, Kees Joost; Sijbers, Jan

2011-09-01

In this paper, we present an iterative reconstruction algorithm for discrete tomography, called discrete algebraic reconstruction technique (DART). DART can be applied if the scanned object is known to consist of only a few different compositions, each corresponding to a constant gray value in the reconstruction. Prior knowledge of the gray values for each of the compositions is exploited to steer the current reconstruction towards a reconstruction that contains only these gray values. Based on experiments with both simulated CT data and experimental μCT data, it is shown that DART is capable of computing more accurate reconstructions from a small number of projection images, or from a small angular range, than alternative methods. It is also shown that DART can deal effectively with noisy projection data and that the algorithm is robust with respect to errors in the estimation of the gray values.

Solving Equations of Multibody Dynamics

NASA Technical Reports Server (NTRS)

Jain, Abhinandan; Lim, Christopher

2007-01-01

Darts++ is a computer program for solving the equations of motion of a multibody system or of a multibody model of a dynamic system. It is intended especially for use in dynamical simulations performed in designing and analyzing, and developing software for the control of, complex mechanical systems. Darts++ is based on the Spatial-Operator- Algebra formulation for multibody dynamics. This software reads a description of a multibody system from a model data file, then constructs and implements an efficient algorithm that solves the dynamical equations of the system. The efficiency and, hence, the computational speed is sufficient to make Darts++ suitable for use in realtime closed-loop simulations. Darts++ features an object-oriented software architecture that enables reconfiguration of system topology at run time; in contrast, in related prior software, system topology is fixed during initialization. Darts++ provides an interface to scripting languages, including Tcl and Python, that enable the user to configure and interact with simulation objects at run time.

The Double Asteroid Redirection Test in the AIDA Mission

NASA Astrophysics Data System (ADS)

Cheng, Andrew; Reed, Cheryl; Rivkin, Andrew

2016-07-01

The Asteroid Impact & Deflection Assessment (AIDA) mission will be the first space experiment to demonstrate asteroid impact hazard mitigation by using a kinetic impactor. AIDA is a joint ESA-NASA cooperative project, consisting of the ESA Asteroid Impact Mission (AIM) rendezvous mission and the NASA Double Asteroid Redirection Test (DART) mission. The AIDA target is the near-Earth binary asteroid 65803 Didymos, which will make an unusually close approach to Earth in October, 2022. The DART spacecraft is designed to impact the Didymos secondary at 7 km/s and demonstrate the ability to modify its trajectory through momentum transfer. DART and AIM are currently Phase A studies supported by NASA and ESA respectively. The primary goals of AIDA are (1) perform a full-scale demonstration of the spacecraft kinetic impact technique for deflection of an asteroid; (2) measure the resulting asteroid deflection, by targeting the secondary member of a binary NEO and measuring the resulting changes of the binary orbit; and (3) study hyper-velocity collision effects on an asteroid, validating models for momentum transfer in asteroid impacts based on measured physical properties of the asteroid surface and sub-surface, and including long-term dynamics of impact ejecta. The primary DART objectives are to demonstrate a hyper-velocity impact on the Didymos moon and to determine the resulting deflection from ground-based observations. The DART impact on the Didymos secondary will change the orbital period of the binary which can be measured by supporting Earth-based optical and radar observations. The baseline DART mission launches in December, 2020 to impact the Didymos secondary in September,2022. There are multiple launch opportunities for DART leading to impact around the 2022 Didymos close approach to Earth. The AIM spacecraft will be launched in Dec. 2020 and arrive at Didymos in spring, 2022, several months before the DART impact. AIM will characterize the Didymos binary system by means of remote sensing and in-situ instruments both before and after the DART impact. The asteroid deflection will be measured to higher accuracy, and additional results of the DART impact, like the impact crater, will be studied in detail by the AIM mission. The combined DART and AIM missions will provide the first measurements of momentum transfer efficiency from hyper-velocity kinetic impact at full scale on an asteroid, where the impact conditions of the projectile are known, and physical properties and internal structures of the target asteroid are also characterized. The DART impact on the Didymos secondary is predicted to cause a 4.4 minute change in the binary orbit period, assuming unit momentum transfer efficiency. The predicted transfer efficiency would be in the range 1.1 to 1.3 for a porous target material based on a variety of numerical and analytical methods, but may be much larger if the target is non-porous. The DART kinetic impact is predicted to make a crater of 6 to 17 meters diameter, depending on target physical properties, but will also release a large volume of particulate ejecta that may be directly observable from Earth or even resolvable as a coma or an ejecta tail by ground-based telescopes.

Ensemble data assimilation in the Red Sea: sensitivity to ensemble selection and atmospheric forcing

NASA Astrophysics Data System (ADS)

Toye, Habib; Zhan, Peng; Gopalakrishnan, Ganesh; Kartadikaria, Aditya R.; Huang, Huang; Knio, Omar; Hoteit, Ibrahim

2017-07-01

We present our efforts to build an ensemble data assimilation and forecasting system for the Red Sea. The system consists of the high-resolution Massachusetts Institute of Technology general circulation model (MITgcm) to simulate ocean circulation and of the Data Research Testbed (DART) for ensemble data assimilation. DART has been configured to integrate all members of an ensemble adjustment Kalman filter (EAKF) in parallel, based on which we adapted the ensemble operations in DART to use an invariant ensemble, i.e., an ensemble Optimal Interpolation (EnOI) algorithm. This approach requires only single forward model integration in the forecast step and therefore saves substantial computational cost. To deal with the strong seasonal variability of the Red Sea, the EnOI ensemble is then seasonally selected from a climatology of long-term model outputs. Observations of remote sensing sea surface height (SSH) and sea surface temperature (SST) are assimilated every 3 days. Real-time atmospheric fields from the National Center for Environmental Prediction (NCEP) and the European Center for Medium-Range Weather Forecasts (ECMWF) are used as forcing in different assimilation experiments. We investigate the behaviors of the EAKF and (seasonal-) EnOI and compare their performances for assimilating and forecasting the circulation of the Red Sea. We further assess the sensitivity of the assimilation system to various filtering parameters (ensemble size, inflation) and atmospheric forcing.

78 FR 14467 - Airworthiness Directives; Slingsby Sailplanes Ltd. Sailplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-06

... (AD) for all Slingsby Sailplanes Ltd. Models Dart T.51, Dart T.51/17, and Dart T.51/ 17R sailplanes... condition as an incident of glue joint failure on a starboard wing caused by water entering the area of the airbrake box that resulted in delamination and corrosion in the area of the aluminum alloy spar booms and...

78 FR 28723 - Airworthiness Directives; Slingsby Sailplanes Ltd. Sailplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-16

... directive (AD) for all Slingsby Sailplanes Ltd. Models Dart T.51, Dart T.51/17, and Dart T.51/17R sailplanes... failure on a starboard wing caused by water entering the area of the airbrake box that resulted in delamination and corrosion in the area of the aluminum alloy spar booms and the wing attach fittings. We are...

Evaluation of ice-tea quality by DART-TOF/MS.

PubMed

Rajchl, Aleš; Prchalová, Jana; Kružík, Vojtěch; Ševčík, Rudolf; Čížková, Helena

2015-11-01

DART (Direct Analysis in Real Time) coupled with Time-of-Flight Mass Spectrometry (TOF/MS) has been used for analyses of ice-teas. The article focuses on quality and authenticity of ice-teas as one of the most important tea-based products on the market. Twenty-one samples of ice-teas (black and green) were analysed. Selected compounds of ice-teas were determined: theobromine, caffeine, total phenolic compounds, total soluble solids, total amino acid concentration, preservatives and saccharides were determined. Fingerprints of DART-TOF/MS spectra were used for comprehensive assessment of the ice-tea samples. The DART-TOF/MS method was used for monitoring the following compounds: citric acid, caffeine, saccharides, artificial sweeteners (saccharin, acesulphame K), and preservatives (sorbic and benzoic acid), phosphoric acid and phenolic compounds. The measured data were subjected to a principal components analysis. The HPLC and DART-TOF/MS methods were compared in terms of determination of selected compounds (caffeine, benzoic acid, sorbic acid and saccharides) in the ice-teas. The DART-TOF/MS technique seems to be a suitable method for fast screening, testing quality and authenticity of tea-based products. Copyright © 2015 John Wiley & Sons, Ltd.

Smart darting diffusion Monte Carlo: Applications to lithium ion-Stockmayer clusters

DOE Office of Scientific and Technical Information (OSTI.GOV)

Christensen, H. M.; Jake, L. C.; Curotto, E., E-mail: curotto@arcadia.edu

2016-05-07

In a recent investigation [K. Roberts et al., J. Chem. Phys. 136, 074104 (2012)], we have shown that, for a sufficiently complex potential, the Diffusion Monte Carlo (DMC) random walk can become quasiergodic, and we have introduced smart darting-like moves to improve the sampling. In this article, we systematically characterize the bias that smart darting moves introduce in the estimate of the ground state energy of a bosonic system. We then test a simple approach to eliminate completely such bias from the results. The approach is applied for the determination of the ground state of lithium ion-n–dipoles clusters in themore » n = 8–20 range. For these, the smart darting diffusion Monte Carlo simulations find the same ground state energy and mixed-distribution as the traditional approach for n < 14. In larger systems we find that while the ground state energies agree quantitatively with or without smart darting moves, the mixed-distributions can be significantly different. Some evidence is offered to conclude that introducing smart darting-like moves in traditional DMC simulations may produce a more reliable ground state mixed-distribution.« less

Analysis and Hindcast Experiments of the 2009 Sudden Stratospheric Warming in WACCMX+DART

NASA Astrophysics Data System (ADS)

Pedatella, N. M.; Liu, H.-L.; Marsh, D. R.; Raeder, K.; Anderson, J. L.; Chau, J. L.; Goncharenko, L. P.; Siddiqui, T. A.

2018-04-01

The ability to perform data assimilation in the Whole Atmosphere Community Climate Model eXtended version (WACCMX) is implemented using the Data Assimilation Research Testbed (DART) ensemble adjustment Kalman filter. Results are presented demonstrating that WACCMX+DART analysis fields reproduce the middle and upper atmosphere variability during the 2009 major sudden stratospheric warming (SSW) event. Compared to specified dynamics WACCMX, which constrains the meteorology by nudging toward an external reanalysis, the large-scale dynamical variability of the stratosphere, mesosphere, and lower thermosphere is improved in WACCMX+DART. This leads to WACCMX+DART better representing the downward transport of chemical species from the mesosphere into the stratosphere following the SSW. WACCMX+DART also reproduces most aspects of the observed variability in ionosphere total electron content and equatorial vertical plasma drift during the SSW. Hindcast experiments initialized on 5, 10, 15, 20, and 25 January are used to assess the middle and upper atmosphere predictability in WACCMX+DART. A SSW, along with the associated middle and upper atmosphere variability, is initially predicted in the hindcast initialized on 15 January, which is ˜10 days prior to the warming. However, it is not until the hindcast initialized on 20 January that a major SSW is forecast to occur. The hindcast experiments reveal that dominant features of the total electron content can be forecasted ˜10-20 days in advance. This demonstrates that whole atmosphere models that properly account for variability in lower atmosphere forcing can potentially extend the ionosphere-thermosphere forecast range.

Fundamentals of ambient metastable-induced chemical ionization mass spectrometry and atmospheric pressure ion mobility spectrometry

NASA Astrophysics Data System (ADS)

Harris, Glenn A.

Molecular ionization is owed much of its development from the early implementation of electron ionization (EI). Although dramatically increasing the library of compounds discovered, an inherent problem with EI was the low abundance of molecular ions detected due to high fragmentation leading to the difficult task of the correct chemical identification after mass spectrometry (MS). These problems stimulated the research into new ionization methods which sought to "soften" the ionization process. In the late 1980s the advancements of ionization techniques was thought to have reached its pinnacle with both electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Both ionization techniques allowed for "soft" ionization of large molecular weight and/or labile compounds for intact characterization by MS. Albeit pervasive, neither ESI nor MALDI can be viewed as "magic bullet" ionization techniques. Both techniques require sample preparation which often included native sample destruction, and operation of these techniques took place in sealed enclosures and often, reduced pressure conditions. New open-air ionization techniques termed "ambient MS" enable direct analysis of samples of various physical states, sizes and shapes. One particular technique named Direct Analysis In Real Time (DART) has been steadily growing as one of the ambient tools of choice to ionize small molecular weight (< 1000 Da) molecules with a wide range of polarities. Although there is a large list of reported applications using DART as an ionization source, there have not been many studies investigating the fundamental properties of DART desorption and ionization mechanisms. The work presented in this thesis is aimed to provide in depth findings on the physicochemical phenomena during open-air DART desorption and ionization MS and current application developments. A review of recent ambient plasma-based desorption/ionization techniques for analytical MS is presented in Chapter 1. Chapter 2 presents the first investigations into the atmospheric pressure ion transport phenomena during DART analysis. Chapter 3 provides a comparison on the internal energy deposition processes during DART and pneumatically assisted-ESI. Chapter 4 investigates the complex spatially-dependent sampling sensitivity, dynamic range and ion suppression effects present in most DART experiments. New implementations and applications with DART are shown in Chapters 5 and 6. In Chapter 5, DART is coupled to multiplexed drift tube ion mobility spectrometry as a potential fieldable platform for the detection of toxic industrial chemicals and chemical warfare agents simulants. In Chapter 6, transmission-mode DART is shown to be an effective method for reproducible sampling from materials which allow for gas to flow through it. Also, Chapter 6 provides a description of a MS imaging platform coupling infrared laser ablation and DART-like phenomena. Finally, in Chapter 7 I will provide perspective on the work completed with DART and the tasks and goals that future studies should focus on.

Analysis of carbohydrates in Fusarium verticillioides using size-exclusion HPLC – DRI and direct analysis in real time ionization – time-of-flight – mass spectrometry (DART-MS)

USDA-ARS?s Scientific Manuscript database

Direct analysis in real time ionization – time-of-flight – mass spectrometry (DART-MS) and size-exclusion HPLC – DRI are used, respectively, to qualitatively and quantitatively determine the carbohydrates extracted from the corn rot fungus Fusarium verticillioides. In situ permethylation in the DART...

Diamond anvils with a round table designed for high pressure experiments in DAC

NASA Astrophysics Data System (ADS)

Dubrovinsky, Leonid; Koemets, Egor; Bykov, Maxim; Bykova, Elena; Aprilis, Georgios; Pakhomova, Anna; Glazyrin, Konstantin; Laskin, Alexander; Prakapenka, Vitali B.; Greenberg, Eran; Dubrovinskaia, Natalia

2017-10-01

Here, we present new Diamond Anvils with a Round Table (DART-anvils) designed for applications in the diamond anvil cell (DAC) technique. The main features of the new DART-anvil design are a spherical shape of both the crown and the table of a diamond and the position of the centre of the culet exactly in the centre of the sphere. The performance of DART-anvils was tested in a number of high pressure high-temperature experiments at different synchrotron beamlines. These experiments demonstrated a number of advantages, which are unavailable with any of the hitherto known anvil designs. Use of DART-anvils enables to realise in situ single-crystal X-ray diffraction experiments with laser heating using stationary laser-heating setups; eliminating flat-plate design of conventional anvils, DART-anvils make the cell alignment easier; working as solid immersion lenses, they provide additional magnification of the sample in a DAC and improve the image resolution.

An alternative fabrication method of the dart thrower's motion orthosis (also known as the dart orthosis).

PubMed

Schwartz, Deborah A

2016-01-01

To allow safe early wrist motion after wrist injury, this author has modified an earlier version of a dart thrower's motion orthotic device using material that is currently available on the market and an inexpensive paper fastener as the rivet. - KristinValdes, OTD, OT, CHT, Practice Forum Editor. Copyright © 2016 Hanley & Belfus. Published by Elsevier Inc. All rights reserved.

Design of the advanced regional aircraft, the DART-75

NASA Technical Reports Server (NTRS)

Elliott, Steve; Gislason, Jason; Huffstetler, Mark; Mann, Jon; Withers, Ashley; Zimmerman, Mark

1992-01-01

This design analysis is intended to show the capabilities of the DART-75, a 75 passenger medium-range regional transport. Included are the detailed descriptions of the structures, performance, stability and control, weight and balance, and engine design. The design should allow for the DART to become the premier regional aircraft of the future due to some advanced features like the canard, semi-composite construction, and advanced engines.

Versatile new ion source for the analysis of materials in open air under ambient conditions.

PubMed

Cody, Robert B; Laramée, James A; Durst, H Dupont

2005-04-15

A new ion source has been developed for rapid, noncontact analysis of materials at ambient pressure and at ground potential. The new source, termed DART (for "Direct Analysis in Real Time"), is based on the reactions of electronic or vibronic excited-state species with reagent molecules and polar or nonpolar analytes. DART has been installed on a high-resolution time-of-flight mass spectrometer (TOFMS) that provides improved selectivity and accurate elemental composition assignment through exact mass measurements. Although DART has been applied to the analysis of gases, liquids, and solids, a unique application is the direct detection of chemicals on surfaces without requiring sample preparation, such as wiping or solvent extraction. DART has demonstrated success in sampling hundreds of chemicals, including chemical agents and their signatures, pharmaceutics, metabolites, peptides and oligosaccharides, synthetic organics, organometallics, drugs of abuse, explosives, and toxic industrial chemicals. These species were detected on various surfaces, such as concrete, asphalt, human skin, currency, airline boarding passes, business cards, fruits, vegetables, spices, beverages, body fluids, horticultural leaves, cocktail glasses, and clothing. DART employs no radioactive components and is more versatile than devices using radioisotope-based ionization. Because its response is instantaneous, DART provides real-time information, a critical requirement for screening or high throughput.

Characteristics of negative lightning leaders to ground observed by TVLS

NASA Astrophysics Data System (ADS)

Qiu, Shi; Jiang, Zhidong; Shi, Lihua; Niu, Zhencong; Zhang, Peng

2015-12-01

The Thunder and VHF lightning Locating System (termed TVLS) is established and utilized to observe leader behaviors of negative cloud to ground (CG) flashes. This system takes advantages of VHF broadband interferometer and thunder imaging technique, which could provide the temporal and quasi-3D spatial evolution of lightning discharges. In conjunction with synchronized electric field changes (E-changes) and electric field derivatives (dE/dt) records, 10 leaders from two CG flashes are presented and analyzed. Based on the characteristic evolution of leader velocities, E-changes, dE/dt waveforms and VHF intervals, three stepped leaders, five dart leaders and two dart-stepped leaders are identified. The stepped leaders behave impulsive while approaching ground, with average speed (1.3∼3.9)×105 m/s. All normal dart leaders presented here exhibit irregular (or termed "chaotic") fluctuations in E-change and dE/dt waveforms, with the similar speeds ((1.0∼2.9)×107 m/s) and durations ((300∼700) μs) of the "chaotic" leaders observed by other investigators. The irregular fluctuations would be weak if the channels keep conductive until the leader enters the less conductive branches, coinciding with VHF radiations in time sequence. The dart-stepped leader could be divided into the dart stage and the stepped stage by a transition region, which usually lies around the branch junctions of previous active channel. The dart stage resembles the normal dart leader, and the stepped stage usually associates with regular pulse trains in E-change and dE/dt waveforms.

Dual-Affinity Re-Targeting proteins direct T cell–mediated cytolysis of latently HIV-infected cells

PubMed Central

Sung, Julia A.M.; Pickeral, Joy; Liu, Liqin; Stanfield-Oakley, Sherry A.; Lam, Chia-Ying Kao; Garrido, Carolina; Pollara, Justin; LaBranche, Celia; Bonsignori, Mattia; Moody, M. Anthony; Yang, Yinhua; Parks, Robert; Archin, Nancie; Allard, Brigitte; Kirchherr, Jennifer; Kuruc, JoAnn D.; Gay, Cynthia L.; Cohen, Myron S.; Ochsenbauer, Christina; Soderberg, Kelly; Liao, Hua-Xin; Montefiori, David; Moore, Paul; Johnson, Syd; Koenig, Scott; Haynes, Barton F.; Nordstrom, Jeffrey L.; Margolis, David M.; Ferrari, Guido

2015-01-01

Enhancement of HIV-specific immunity is likely required to eliminate latent HIV infection. Here, we have developed an immunotherapeutic modality aimed to improve T cell–mediated clearance of HIV-1–infected cells. Specifically, we employed Dual-Affinity Re-Targeting (DART) proteins, which are bispecific, antibody-based molecules that can bind 2 distinct cell-surface molecules simultaneously. We designed DARTs with a monovalent HIV-1 envelope-binding (Env-binding) arm that was derived from broadly binding, antibody-dependent cellular cytotoxicity–mediating antibodies known to bind to HIV-infected target cells coupled to a monovalent CD3 binding arm designed to engage cytolytic effector T cells (referred to as HIVxCD3 DARTs). Thus, these DARTs redirected polyclonal T cells to specifically engage with and kill Env-expressing cells, including CD4+ T cells infected with different HIV-1 subtypes, thereby obviating the requirement for HIV-specific immunity. Using lymphocytes from patients on suppressive antiretroviral therapy (ART), we demonstrated that DARTs mediate CD8+ T cell clearance of CD4+ T cells that are superinfected with the HIV-1 strain JR-CSF or infected with autologous reservoir viruses isolated from HIV-infected–patient resting CD4+ T cells. Moreover, DARTs mediated CD8+ T cell clearance of HIV from resting CD4+ T cell cultures following induction of latent virus expression. Combined with HIV latency reversing agents, HIVxCD3 DARTs have the potential to be effective immunotherapeutic agents to clear latent HIV-1 reservoirs in HIV-infected individuals. PMID:26413868

Acoustic vs Interferometric Measurements of Lightning

NASA Astrophysics Data System (ADS)

Arechiga, R. O.; Erives, H.; Sonnenfeld, R. G.; Stanley, M. A.; Rison, W.; Thomas, R. J.; Edens, H. E.; Lapierre, J. L.; Stock, M.; Jensen, D.; Morris, K.

2015-12-01

During the summer of 2015 we acquired acoustic and RF data on severalflashes from thunderstorms over Fort Morgan CO. and Langmuir Laboratoryin the Magdalena mountains of central New Mexico. The acoustic arrayswere located at a distance of roughly 150 m from the interferometers.Lightning mapping array and slow antenna data were also obtained. Theacoustic arrays consist of arrays of five audio-range and six infrasoundmicrophones operating at 50 KHz and 1 KHz respectively. The lightninginterferometer at Fort Morgan CO. consists of three flat-plate, 13" diameterantennas at the vertices of an equilateral 50 m per side triangle. Theinterferometer at Langmuir Laboratory consists of three 13" dishes separatedby about 15 m. Both interferometers, operating at 180 Megasamples persecond, use the analysis software and digitizer hardware pioneered byStanley, Stock et al. The high data rate allows for excellent spatialresolution of high speed (and typically high current) processes such asK-changes, return strokes and dart-leaders. In previous studies, we haveshown the usefulness of acoustic recordings to locate thunder sources aswell as infrasound pulses from lightning. This work will present acomparison of Acoustic and Interferometric measurements from lightning,using some interesting flashes, including a positive cloud to ground,that occurred in these campaigns.

Behavioral and Physiological Response of Baleen Whales to Ships and Ship Noise

DTIC Science & Technology

2014-09-30

student Angela Szesciorka) on tag designs to provide longer term deployments of archival tags especially for work with humpback whales off northern...California. 2. Conduct test deployments of dart-attached archival tags in 2014 on humpback whales in Monterey Bay and the Gulf of the Farallones on... humpback whales to tests different dart attachment configurations and develop a new longer term dart-attached archival tag system. 3. Successfully

Variation of the channel temperature in the transmission of lightning leader

NASA Astrophysics Data System (ADS)

Chang, Xuan; Yuan, Ping; Cen, Jianyong; Wang, Xuejuan

2017-06-01

According to the time-resolved spectra of the lightning stepped leader and dart leader processes, the channel temperature, its evolution characteristics with time and the variation along the channel height in the transmission process were analyzed. The results show that the stepped leader tip has a slightly higher temperature than the trailing end, which should be caused by a large amount of electric charges on the leader tip. In addition, both temperature and brightness are enhanced at the position of the channel node. The dart leader has a higher channel temperature than the stepped leader but a lower temperature than the return stroke. Meanwhile, the channel temperature of the dart leader obviously increases when the dart leader propagates to the ground.

Boeing's Dart and Starliner Parachute System Test

NASA Image and Video Library

2018-02-22

Boeing conducted the first in a series of reliability tests of its CST-100 Starliner flight drogue and main parachute system by releasing a long, dart-shaped test vehicle from a C-17 aircraft over Yuma, Arizona. Two more tests are planned using the dart module, as well as three similar reliability tests using a high fidelity capsule simulator designed to simulate the CST-100 Starliner capsule’s exact shape and mass. In both the dart and capsule simulator tests, the test spacecraft are released at various altitudes to test the parachute system at different deployment speeds, aerodynamic loads, and or weight demands. Data collected from each test is fed into computer models to more accurately predict parachute performance and to verify consistency from test to test.

Rapid detection of hazardous chemicals in textiles by direct analysis in real-time mass spectrometry (DART-MS).

PubMed

Antal, Borbála; Kuki, Ákos; Nagy, Lajos; Nagy, Tibor; Zsuga, Miklós; Kéki, Sándor

2016-07-01

Residues of chemicals on clothing products were examined by direct analysis in real-time (DART) mass spectrometry. Our experiments have revealed the presence of more than 40 chemicals in 15 different clothing items. The identification was confirmed by DART tandem mass spectrometry (MS/MS) experiments for 14 compounds. The most commonly detected hazardous substances were nonylphenol ethoxylates (NPEs), phthalic acid esters (phthalates), amines released by azo dyes, and quinoline derivates. DART-MS was able to detect NPEs on the skin of the person wearing the clothing item contaminated by NPE residuals. Automated data acquisition and processing method was developed and tested for the recognition of NPE residues thereby reducing the analysis time.

Revision of the ICH guideline on detection of toxicity to reproduction for medicinal products: SWOT analysis.

PubMed

Barrow, Paul

2016-09-01

SWOT analysis was used to gain insights and perspectives into the revision of the ICH S5(R2) guideline on detection of toxicity to reproduction for medicinal products. The current ICH guideline was rapidly adopted worldwide and has an excellent safety record for more than 20 years. The revised guideline should aim to further improve reproductive and developmental (DART) safety testing for new drugs. Alternative methods to animal experiments should be used whenever possible. Modern technology should be used to obtain high quality data from fewer animals. Additions to the guideline should include considerations on the following: limit dose setting, maternal toxicity, biopharmaceuticals, vaccines, testing strategies by indication, developmental immunotoxicity, and male-mediated developmental toxicity. Emerging issues, such as epigenetics and the microbiome, will most likely pose challenges to DART testing in the future. It is hoped that the new guideline will be adopted even outside the ICH regions. Copyright © 2016 Elsevier Inc. All rights reserved.

"Soft"or "hard" ionisation? Investigation of metastable gas temperature effect on direct analysis in real-time analysis of Voriconazole.

PubMed

Lapthorn, Cris; Pullen, Frank

2009-01-01

The performance of the direct analysis in real-time (DART) technique was evaluated across a range of metastable gas temperatures for a pharmaceutical compound, Voriconazole, in order to investigate the effect of metastable gas temperature on molecular ion intensity and fragmentation. The DART source has been used to analyse a range of analytes and from a range of matrices including drugs in solid tablet form and preparations, active ingredients in ointment, naturally occurring plant alkaloids, flavours and fragrances, from thin layer chromatography (TLC) plates, melting point tubes and biological matrices including hair, urine and blood. The advantages of this technique include rapid analysis time (as little as 5 s), a reduction in sample preparation requirements, elimination of mobile phase requirement and analysis of samples not typically amenable to atmospheric pressure ionisation (API) techniques. This technology has therefore been proposed as an everyday tool for identification of components in crude organic reaction mixtures.

Paper Darts

ERIC Educational Resources Information Center

Tapson, Frank

1974-01-01

Motivation for practicing basic arithmetic skills is provided by activities based on dart board games. There activities also require participants to devise winning strategies, adding enrichment to the game-type drills. (JP)

Ionization Mechanism of Positive-Ion Nitrogen Direct Analysis in Real Time.

PubMed

Song, Liguo; Chuah, Wei Chean; Lu, Xinyi; Remsen, Edward; Bartmess, John E

2018-04-01

Nitrogen can be an inexpensive alternative to helium used by direct analysis in real time (DART), especially in consideration of the looming helium shortage. Therefore, the ionization mechanism of positive-ion N 2 DART has been systematically investigated. Our experiments suggest that a range of metastable nitrogen species with a variety of internal energies existed and all of them were less energetic than metastable helium atoms. However, compounds with ionization energies (IE) equal to or lower than 10.2 eV (all organic compounds except the extremely small ones) can be efficiently ionized. Because N 2 DART was unable to efficiently ionize ambient moisture and common organic solvents such as methanol and acetonitrile, the most important ionization mechanism was direct Penning ionization followed by self-protonation of polar compounds generating [M+H] + ions. On the other hand, N 2 DART was able to efficiently ionize ammonia, which was beneficial in the ionization of hydrogen-bonding compounds with proton affinities (PA) weaker than ammonia generating [M+NH 4 ] + ions and large PAHs generating [M+H] + ions through proton transfer. N 2 DART was also able to efficiently ionize NO, which led to the ionization of nonpolar compounds such as alkanes and small aromatics generating [M-(2m+1)H] + (m=0,1…) ions. Lastly, metastable nitrogen species was also able to produce oxygen atoms, which resulted in increased oxygen adducts as the polarity of organic compounds decreased. In comparison with He DART, N 2 DART was approximately one order of magnitude less sensitive in generating [M+H] + ions, but could be more sensitive in generating [M+NH 4 ] + ions. Graphical Abstract ᅟ.

Ionization Mechanism of Positive-Ion Nitrogen Direct Analysis in Real Time

NASA Astrophysics Data System (ADS)

Song, Liguo; Chuah, Wei Chean; Lu, Xinyi; Remsen, Edward; Bartmess, John E.

2018-02-01

Nitrogen can be an inexpensive alternative to helium used by direct analysis in real time (DART), especially in consideration of the looming helium shortage. Therefore, the ionization mechanism of positive-ion N2 DART has been systematically investigated. Our experiments suggest that a range of metastable nitrogen species with a variety of internal energies existed and all of them were less energetic than metastable helium atoms. However, compounds with ionization energies (IE) equal to or lower than 10.2 eV (all organic compounds except the extremely small ones) can be efficiently ionized. Because N2 DART was unable to efficiently ionize ambient moisture and common organic solvents such as methanol and acetonitrile, the most important ionization mechanism was direct Penning ionization followed by self-protonation of polar compounds generating [M+H]+ ions. On the other hand, N2 DART was able to efficiently ionize ammonia, which was beneficial in the ionization of hydrogen-bonding compounds with proton affinities (PA) weaker than ammonia generating [M+NH4]+ ions and large PAHs generating [M+H]+ ions through proton transfer. N2 DART was also able to efficiently ionize NO, which led to the ionization of nonpolar compounds such as alkanes and small aromatics generating [M-(2m+1)H]+ (m=0,1…) ions. Lastly, metastable nitrogen species was also able to produce oxygen atoms, which resulted in increased oxygen adducts as the polarity of organic compounds decreased. In comparison with He DART, N2 DART was approximately one order of magnitude less sensitive in generating [M+H]+ ions, but could be more sensitive in generating [M+NH4]+ ions. [Figure not available: see fulltext.

Remote chemical immobilisation method for free-ranging Australian cattle.

PubMed

Hampton, J O; Skroblin, A; Perry, A L; De Ridder, T R

2016-12-01

Many situations are encountered in Australia where the capture and restraint of free-ranging cattle (Bos taurus/Bos indicus) is required. Chemical immobilisation via darting is a potentially useful tool for managing and researching large wild herbivores; however, there is no reliable method for its application to Australian cattle. The aim of this study was to develop an efficacious, humane, cost-effective ground darting method for free-ranging cattle. The 30 female cattle were darted and captured on a pastoral station in north-west Australia from a vehicle. Xylazine (0.59 mg/kg) and ketamine (3.59 mg/kg) were used to capture animals and yohimbine (0.10 mg/kg) was used as an antagonist to xylazine to reduce recumbent time. Cattle became recumbent at a mean time of 8 min and a mean distance of 260 m from darting. The mortality rate was zero on the day of capture and 7% at 14 days post-capture. The majority of darted cattle were successfully immobilised with one dart and recovered within 30 min, with consumables costing approximately A$30 per captured animal. The technique developed represents a rapid and humane method for capturing free-ranging cattle and, with consideration for legislation surrounding use of veterinary chemicals, could be applied in many contexts across Australia. © 2016 Australian Veterinary Association.

Analysis of the cyanolichen Lichina pygmaea metabolites using in situ DART-MS: from detection to thermochemistry of mycosporine serinol.

PubMed

Le Pogam, Pierre; Legouin, Béatrice; Le Lamer, Anne-Cécile; Boustie, Joël; Rondeau, David

2015-03-01

Direct Analysis in Real Time DART-HRMS is here first applied to the detection of molecules from a lichen, Lichina pygmaea. The aim was to propose an innovative method of in situ detection of lichen secondary metabolites using the possibilities of elemental composition determination available when a DART source is interfaced with a TOF analyzer. Three kinds of samples have been submitted to DART ionization, i.e. an intact thallus, a powder obtained from the crushed lichen and an aqueous extract. In situ analysis of crushed lichen, yields an extensive chemical profile, comparable to what is obtained from the aqueous extract, comprising both major polar metabolites described in literature along with some other signals that could correspond to potentially unknown metabolites. One of the detected secondary metabolites, mycosporine serinol, underwent a dehydration reaction prior to its transfer in the gas-phase by DART ionization. The consideration of the thermal transfers involved in the DART ionization process and the possibility to record time-dependent mass spectra through the use of the TOF analyzer allowed establishing Arrhenius plots of this water molecule loss to obtain associated thermodynamic quantities. The low values of corresponding activation enthalpy (Δr‡Hm° of the order of 25 kJ mol(-1)) enabled formulating some assumption regarding a possible role of such metabolites in the lichen. Copyright © 2015 John Wiley & Sons, Ltd.

Determination of the aflatoxin AFB1 from corn by direct analysis in real time-mass spectrometry (DART-MS).

PubMed

Busman, Mark; Liu, Jihong; Zhong, Hongjian; Bobell, John R; Maragos, Chris M

2014-01-01

Direct analysis in real time (DART) ionisation coupled to a high-resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of a common form of aflatoxin, AFB1, extracted from corn. Sample preparation procedure and instrument parameter settings were optimised to obtain sensitive and accurate determination of aflatoxin AFB1. 84:16 acetonitrile water extracts of corn were analysed by DART-MS. The lowest calibration level (LCL) for aflatoxin AFB1 was 4 μg kg⁻¹. Quantitative analysis was performed with the use of matrix-matched standards employing the ¹³C-labelled internal standard for AFB1. DART-MS of spiked corn extracts gave linear response in the range 4-1000 μg kg⁻¹. Good recoveries (94-110%) and repeatabilities (RSD = 0.7-6.9%) were obtained at spiking levels of 20 and 100 μg kg⁻¹ with the use of an isotope dilution technique. Trueness of data obtained for AFB1 in maize by DART-MS was demonstrated by analysis of corn certified reference materials.

Homicide by Sch from a syringe-like dart ejected by a compound crossbow.

PubMed

Guo, Wei; Luo, Guochang; Wang, Hao; Meng, Xiangzhi

2015-02-01

The compound crossbow can be used to eject syringe-like dart loaded with poisonous solution. Succinylcholine (Sch) is a short-acting neuromuscular blocker medically used to achieve complete relaxation of muscle for a good intubation condition. Without the help of an artificial respirator, intramuscular injection of a large dose of Sch can paralyze the respiratory muscle and result in the receiver's death. In this paper, we present the homicide case of a young male killed by Sch from a syringe-like dart ejected by a compound crossbow. The subcutaneous and muscular hemorrhages observed around the entry were more severe than that caused by a medical injection. Additionally, other autopsy results showed the external appearance of a pinhole, general asphyxia signs and pathological findings which were not characteristic. The discovery of a syringe-like dart at the scene is the critical clue and reason for analyzing for Sch, which is commonly used to load syringe-like dart to paralyze and steal dog in the countryside of China. Copyright © 2014 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

Scaphoid tuberosity excursion is minimized during a dart-throwing motion: A biomechanical study.

PubMed

Werner, Frederick W; Sutton, Levi G; Basu, Niladri; Short, Walter H; Moritomo, Hisao; St-Amand, Hugo

2016-01-01

The purpose of this study was to determine whether the excursion of the scaphoid tuberosity and therefore scaphoid motion is minimized during a dart-throwing motion. Scaphoid tuberosity excursion was studied as an indicator of scaphoid motion in 29 cadaver wrists as they were moved through wrist flexion-extension, radioulnar deviation, and a dart-throwing motion. Study results demonstrate that excursion was significantly less during the dart-throwing motion than during either wrist flexion-extension or radioulnar deviation. If the goal of early wrist motion after carpal ligament or distal radius injury and reconstruction is to minimize loading of the healing structures, a wrist motion in which scaphoid motion is minimal should reduce length changes in associated ligamentous structures. Therefore, during rehabilitation, if a patient uses a dart-throwing motion that minimizes his or her scaphoid tuberosity excursion, there should be minimal changes in ligament loading while still allowing wrist motion. Bench research, biomechanics, and cross-sectional. Not applicable. The study was laboratory based. Copyright © 2016 Hanley & Belfus. Published by Elsevier Inc. All rights reserved.

Rapid Analysis of Trace Drugs and Metabolites Using a Thermal Desorption DART-MS Configuration.

PubMed

Sisco, Edward; Forbes, Thomas P; Staymates, Matthew E; Gillen, Greg

2016-01-01

The need to analyze trace narcotic samples rapidly for screening or confirmatory purposes is of increasing interest to the forensic, homeland security, and criminal justice sectors. This work presents a novel method for the detection and quantification of trace drugs and metabolites off of a swipe material using a thermal desorption direct analysis in real time mass spectrometry (TD-DART-MS) configuration. A variation on traditional DART, this configuration allows for desorption of the sample into a confined tube, completely independent of the DART source, allowing for more efficient and thermally precise analysis of material present on a swipe. Over thirty trace samples of narcotics, metabolites, and cutting agents deposited onto swipes were rapidly differentiated using this methodology. The non-optimized method led to sensitivities ranging from single nanograms to hundreds of picograms. Direct comparison to traditional DART with a subset of the samples highlighted an improvement in sensitivity by a factor of twenty to thirty and an increase in reproducibility sample to sample from approximately 45 % RSD to less than 15 % RSD. Rapid extraction-less quantification was also possible.

Impacts of Population Structure and Analytical Models in Genome-Wide Association Studies of Complex Traits in Forest Trees: A Case Study in Eucalyptus globulus

PubMed Central

Garcia, Martín N.; Acuña, Cintia; Borralho, Nuno M. G.; Grattapaglia, Dario; Marcucci Poltri, Susana N.

2013-01-01

The promise of association genetics to identify genes or genomic regions controlling complex traits has generated a flurry of interest. Such phenotype-genotype associations could be useful to accelerate tree breeding cycles, increase precision and selection intensity for late expressing, low heritability traits. However, the prospects of association genetics in highly heterozygous undomesticated forest trees can be severely impacted by the presence of cryptic population and pedigree structure. To investigate how to better account for this, we compared the GLM and five combinations of the Unified Mixed Model (UMM) on data of a low-density genome-wide association study for growth and wood property traits carried out in a Eucalyptus globulus population (n = 303) with 7,680 Diversity Array Technology (DArT) markers. Model comparisons were based on the degree of deviation from the uniform distribution and estimates of the mean square differences between the observed and expected p-values of all significant marker-trait associations detected. Our analysis revealed the presence of population and family structure. There was not a single best model for all traits. Striking differences in detection power and accuracy were observed among the different models especially when population structure was not accounted for. The UMM method was the best and produced superior results when compared to GLM for all traits. Following stringent correction for false discoveries, 18 marker-trait associations were detected, 16 for tree diameter growth and two for lignin monomer composition (S∶G ratio), a key wood property trait. The two DArT markers associated with S∶G ratio on chromosome 10, physically map within 1 Mbp of the ferulate 5-hydroxylase (F5H) gene, providing a putative independent validation of this marker-trait association. This study details the merit of collectively integrate population structure and relatedness in association analyses in undomesticated, highly heterozygous forest trees, and provides additional insights into the nature of complex quantitative traits in Eucalyptus. PMID:24282578

Quantification of patulin in fruit leathers by ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA).

PubMed

Maragos, Chris M; Busman, Mark; Ma, Liang; Bobell, John

2015-01-01

Patulin is a mycotoxin commonly found in certain fruit and fruit products. For this reason many countries have established regulatory limits pertaining to, in particular, apple juice and apple products. Fruit leathers are produced by dehydrating fruit puree, leaving a sweet product that has a leathery texture. A recent report in the literature described the detection of patulin at substantial levels in fruit leathers. To investigate this further, an ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA) method was developed for the sensitive detection of patulin in fruit leathers. Investigations were also made of the suitability of direct analysis in real time-mass spectrometry (DART-MS) for detection of patulin from the surface of fruit leathers. Results indicated DART-MS was insufficiently sensitive for quantification from the surface of home-style apple leathers, although patulin spiked onto the surface of leather or peel could be detected. The UPLC-PDA method was used to determine the fate of patulin during the preparation of home-made fruit leathers. Interestingly, when a home-style process was used, the patulin was not destroyed, but rather increased in concentration as the puree was dehydrated. The UPLC-PDA method was also used to screen for patulin in commercial fruit leathers. Of the 36 products tested, 14 were above the limit of detection (3.5 μg kg(-1)) and nine were above the limit of quantification (12 μg kg(-1)). Positive samples were confirmed by UPLC-MS/MS. Only one sample was found above the US regulatory limit for single-strength apple juice products (50 μg kg(-1)). These results suggest patulin can be concentrated during preparation and can be found in fruit leathers. The limited survey suggests that patulin is fairly prevalent in such commercial products, but that the levels are usually low.

An electrodynamic description of lightning return strokes and dart leaders: Guided wave propagation along conducting cylindrical channels

DOE Office of Scientific and Technical Information (OSTI.GOV)

Borovsky, J.E.

1995-02-20

The return-stroke breakdown pulse and the dart leader are treated as electric waves guided by conducting lightning channels; such waves are launched when current is injected into a conducting channel (producing the dart leader) or when charge on a channel begins to drain to Earth (producing the return stroke). The guided waves are self-consistent solutions to the full set of Maxwell`s equations, obeying the physical boundary conditions for cylindrical channels. These waves are shown (1) to move with velocities substantially slower than c along the channel, (2) to push current inside the lightning channel, (3) to move charge and voltagemore » along the channel, and (4) to transport energy along and into the channel via Poynting flux. The velocity of a guided wave is a function of only three parameters: the channel radius r{sub ch}, the channel temperature T, and the risetime {triangle}t of the wave front. These velocities are found to fall in the range of velocities of return strokes and of dart leaders. The dart leader and the return stroke are caused by the same type of guided electromagnetic waves: the difference in velocity is owed mostly to the difference in channel temperature. In the case of the dart leader the waves deliver Poynting flux along the outside of the channel down from a thundercloud generator to the downward-propagating wave front. At the wave front of the dart leader the delivered energy goes into heating the channel and into storage in the form of E{sup 2}/8{pi} around the newly charged channel. In the case of the return stroke the Poynting flux is localized to the vicinity of the wave front where stored energy E{sup 2}/8{pi} is delivered radially inward onto the channel to heat the channel in the propagating front. The net result of a dart leader and return stroke is that charge is moved from the cloud to the ground and that energy is moved from the cloud onto the channel. 123 refs., 11 figs., 5 tabs.« less

76 FR 542 - Marine Mammals; File No. 15616

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-05

... recording, biopsy sampling, tagging with barbed darts and suction cups, and collecting samples of marine... acoustically record (PI), biopsy sample (BS), attach barbed dart satellite tags (DT), and suction cup tags (ST...

50 CFR 230.7 - Salvage of stinkers.

Code of Federal Regulations, 2014 CFR

2014-10-01

... the Assistant Administrator or his/her representative each harpoon, lance, or explosive dart found in... has been struck by the captain whose mark appears on the harpoon, lance, or explosive dart found in or...

50 CFR 230.7 - Salvage of stinkers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... the Assistant Administrator or his/her representative each harpoon, lance, or explosive dart found in... has been struck by the captain whose mark appears on the harpoon, lance, or explosive dart found in or...

50 CFR 230.7 - Salvage of stinkers.

Code of Federal Regulations, 2013 CFR

2013-10-01

... the Assistant Administrator or his/her representative each harpoon, lance, or explosive dart found in... has been struck by the captain whose mark appears on the harpoon, lance, or explosive dart found in or...

50 CFR 635.32 - Specifically authorized activities.

Code of Federal Regulations, 2014 CFR

2014-10-01

... public display are required to have either a conventional dart tag or a microchip Passive Integrated... supplied by NMFS. Conventional dart tags will be issued unless PIT tags are specifically requested in the...

50 CFR 635.32 - Specifically authorized activities.

Code of Federal Regulations, 2012 CFR

2012-10-01

... public display are required to have either a conventional dart tag or a microchip Passive Integrated... supplied by NMFS. Conventional dart tags will be issued unless PIT tags are specifically requested in the...

50 CFR 230.7 - Salvage of stinkers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... the Assistant Administrator or his/her representative each harpoon, lance, or explosive dart found in... has been struck by the captain whose mark appears on the harpoon, lance, or explosive dart found in or...

50 CFR 230.7 - Salvage of stinkers.

Code of Federal Regulations, 2012 CFR

2012-10-01

... the Assistant Administrator or his/her representative each harpoon, lance, or explosive dart found in... has been struck by the captain whose mark appears on the harpoon, lance, or explosive dart found in or...

Optimization of a direct analysis in real time/time-of-flight mass spectrometry method for rapid serum metabolomic fingerprinting.

PubMed

Zhou, Manshui; McDonald, John F; Fernández, Facundo M

2010-01-01

Metabolomic fingerprinting of bodily fluids can reveal the underlying causes of metabolic disorders associated with many diseases, and has thus been recognized as a potential tool for disease diagnosis and prognosis following therapy. Here we report a rapid approach in which direct analysis in real time (DART) coupled with time-of-flight (TOF) mass spectrometry (MS) and hybrid quadrupole TOF (Q-TOF) MS is used as a means for metabolomic fingerprinting of human serum. In this approach, serum samples are first treated to precipitate proteins, and the volatility of the remaining metabolites increased by derivatization, followed by DART MS analysis. Maximum DART MS performance was obtained by optimizing instrumental parameters such as ionizing gas temperature and flow rate for the analysis of identical aliquots of a healthy human serum samples. These variables were observed to have a significant effect on the overall mass range of the metabolites detected as well as the signal-to-noise ratios in DART mass spectra. Each DART run requires only 1.2 min, during which more than 1500 different spectral features are observed in a time-dependent fashion. A repeatability of 4.1% to 4.5% was obtained for the total ion signal using a manual sampling arm. With the appealing features of high-throughput, lack of memory effects, and simplicity, DART MS has shown potential to become an invaluable tool for metabolomic fingerprinting. 2010 American Society for Mass Spectrometry. Published by Elsevier Inc. All rights reserved.

Simulation studies using multibody dynamics code DART

NASA Technical Reports Server (NTRS)

Keat, James E.

1989-01-01

DART is a multibody dynamics code developed by Photon Research Associates for the Air Force Astronautics Laboratory (AFAL). The code is intended primarily to simulate the dynamics of large space structures, particularly during the deployment phase of their missions. DART integrates nonlinear equations of motion numerically. The number of bodies in the system being simulated is arbitrary. The bodies' interconnection joints can have an arbitrary number of degrees of freedom between 0 and 6. Motions across the joints can be large. Provision for simulating on-board control systems is provided. Conservation of energy and momentum, when applicable, are used to evaluate DART's performance. After a brief description of DART, studies made to test the program prior to its delivery to AFAL are described. The first is a large angle reorientating of a flexible spacecraft consisting of a rigid central hub and four flexible booms. Reorientation was accomplished by a single-cycle sine wave shape torque input. In the second study, an appendage, mounted on a spacecraft, was slewed through a large angle. Four closed-loop control systems provided control of this appendage and of the spacecraft's attitude. The third study simulated the deployment of the rim of a bicycle wheel configuration large space structure. This system contained 18 bodies. An interesting and unexpected feature of the dynamics was a pulsing phenomena experienced by the stays whole playout was used to control the deployment. A short description of the current status of DART is given.

Disturbance automated reference toolset (DART): Assessing patterns in ecological recovery from energy development on the Colorado Plateau

USGS Publications Warehouse

Nauman, Travis; Duniway, Michael C.; Villarreal, Miguel; Poitras, Travis

2017-01-01

A new disturbance automated reference toolset (DART) was developed to monitor human land surface impacts using soil-type and ecological context. DART identifies reference areas with similar soils, topography, and geology; and compares the disturbance condition to the reference area condition using a quantile-based approach based on a satellite vegetation index. DART was able to represent 26–55% of variation of relative differences in bare ground and 26–41% of variation in total foliar cover when comparing sites with nearby ecological reference areas using the Soil Adjusted Total Vegetation Index (SATVI). Assessment of ecological recovery at oil and gas pads on the Colorado Plateau with DART revealed that more than half of well-pads were below the 25th percentile of reference areas. Machine learning trend analysis of poorly recovering well-pads (quantile < 0.23) had out-of-bag error rates between 37 and 40% indicating moderate association with environmental and management variables hypothesized to influence recovery. Well-pads in grasslands (median quantile [MQ] = 13%), blackbrush (Coleogyne ramosissima) shrublands (MQ = 18%), arid canyon complexes (MQ = 18%), warmer areas with more summer-dominated precipitation, and state administered areas (MQ = 12%) had low recovery rates. Results showcase the usefulness of DART for assessing discrete surface land disturbances, and highlight the need for more targeted rehabilitation efforts at oil and gas well-pads in the arid southwest US.

Disturbance automated reference toolset (DART): Assessing patterns in ecological recovery from energy development on the Colorado Plateau.

PubMed

Nauman, Travis W; Duniway, Michael C; Villarreal, Miguel L; Poitras, Travis B

2017-04-15

A new disturbance automated reference toolset (DART) was developed to monitor human land surface impacts using soil-type and ecological context. DART identifies reference areas with similar soils, topography, and geology; and compares the disturbance condition to the reference area condition using a quantile-based approach based on a satellite vegetation index. DART was able to represent 26-55% of variation of relative differences in bare ground and 26-41% of variation in total foliar cover when comparing sites with nearby ecological reference areas using the Soil Adjusted Total Vegetation Index (SATVI). Assessment of ecological recovery at oil and gas pads on the Colorado Plateau with DART revealed that more than half of well-pads were below the 25th percentile of reference areas. Machine learning trend analysis of poorly recovering well-pads (quantile<0.23) had out-of-bag error rates between 37 and 40% indicating moderate association with environmental and management variables hypothesized to influence recovery. Well-pads in grasslands (median quantile [MQ]=13%), blackbrush (Coleogyne ramosissima) shrublands (MQ=18%), arid canyon complexes (MQ=18%), warmer areas with more summer-dominated precipitation, and state administered areas (MQ=12%) had low recovery rates. Results showcase the usefulness of DART for assessing discrete surface land disturbances, and highlight the need for more targeted rehabilitation efforts at oil and gas well-pads in the arid southwest US. Published by Elsevier B.V.

Pharmaceutical identifier confirmation via DART-TOF.

PubMed

Easter, Jacob L; Steiner, Robert R

2014-07-01

Pharmaceutical analysis comprises a large amount of the casework in forensic controlled substances laboratories. In order to reduce the time of analysis for pharmaceuticals, a Direct Analysis in Real Time ion source coupled with an accurate mass time-of-flight (DART-TOF) mass spectrometer was used to confirm identity. DART-TOF spectral data for pharmaceutical samples were analyzed and evaluated by comparison to standard spectra. Identical mass pharmaceuticals were differentiated using collision induced dissociation fragmentation, present/absent ions, and abundance comparison box plots; principal component analysis (PCA) and linear discriminant analysis (LDA) were used for differentiation of identical mass mixed drug spectra. Mass assignment reproducibility and robustness tests were performed on the DART-TOF spectra. Impacts on the forensic science community include a decrease in analysis time over the traditional gas chromatograph/mass spectrometry (GC/MS) confirmations, better laboratory efficiency, and simpler sample preparation. Using physical identifiers and the DART-TOF to confirm pharmaceutical identity will eliminate the use of GC/MS and effectively reduce analysis time while still complying with accepted analysis protocols. This will prove helpful in laboratories with large backlogs and will simplify the confirmation process. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Electro-thermal vaporization direct analysis in real time-mass spectrometry for water contaminant analysis during space missions.

PubMed

Dwivedi, Prabha; Gazda, Daniel B; Keelor, Joel D; Limero, Thomas F; Wallace, William T; Macatangay, Ariel V; Fernández, Facundo M

2013-10-15

The development of a direct analysis in real time-mass spectrometry (DART-MS) method and first prototype vaporizer for the detection of low molecular weight (∼30-100 Da) contaminants representative of those detected in water samples from the International Space Station is reported. A temperature-programmable, electro-thermal vaporizer (ETV) was designed, constructed, and evaluated as a sampling interface for DART-MS. The ETV facilitates analysis of water samples with minimum user intervention while maximizing analytical sensitivity and sample throughput. The integrated DART-ETV-MS methodology was evaluated in both positive and negative ion modes to (1) determine experimental conditions suitable for coupling DART with ETV as a sample inlet and ionization platform for time-of-flight MS, (2) to identify analyte response ions, (3) to determine the detection limit and dynamic range for target analyte measurement, and (4) to determine the reproducibility of measurements made with the method when using manual sample introduction into the vaporizer. Nitrogen was used as the DART working gas, and the target analytes chosen for the study were ethyl acetate, acetone, acetaldehyde, ethanol, ethylene glycol, dimethylsilanediol, formaldehyde, isopropanol, methanol, methylethyl ketone, methylsulfone, propylene glycol, and trimethylsilanol.

Remote biopsy darting and marking of polar bears

USGS Publications Warehouse

Pagano, Anthony M.; Peacock, Elizabeth; McKinney, Melissa A.

2014-01-01

Remote biopsy darting of polar bears (Ursus maritimus) is less invasive and time intensive than physical capture and is therefore useful when capture is challenging or unsafe. We worked with two manufacturers to develop a combination biopsy and marking dart for use on polar bears. We had an 80% success rate of collecting a tissue sample with a single biopsy dart and collected tissue samples from 143 polar bears on land, in water, and on sea ice. Dye marks ensured that 96% of the bears were not resampled during the same sampling period, and we recovered 96% of the darts fired. Biopsy heads with 5 mm diameters collected an average of 0.12 g of fur, tissue, and subcutaneous adipose tissue, while biopsy heads with 7 mm diameters collected an average of 0.32 g. Tissue samples were 99.3% successful (142 of 143 samples) in providing a genetic and sex identification of individuals. We had a 64% success rate collecting adipose tissue and we successfully examined fatty acid signatures in all adipose samples. Adipose lipid content values were lower compared to values from immobilized or harvested polar bears, indicating that our method was not suitable for quantifying adipose lipid content.

Petroleomics by Direct Analysis in Real Time-Mass Spectrometry.

PubMed

Romão, Wanderson; Tose, Lilian V; Vaz, Boniek G; Sama, Sara G; Lobinski, Ryszard; Giusti, Pierre; Carrier, Hervé; Bouyssiere, Brice

2016-01-01

The analysis of crude oil and its fractions by applying ambient ionization techniques remains underexplored in mass spectrometry (MS). Direct analysis in real time (DART) in the positive-ion mode was coupled to a linear quadrupole ion trap Orbitrap mass spectrometer (LTQ Orbitrap) to analyze crude oil, paraffin samples, and porphyrin standard compounds. The ionization parameters of DART-MS were optimized for crude oil analysis. DART-MS rendered the optimum conditions of the operation using paper as the substrate, T = 400°C, helium as the carrier gas, and a sample concentration ≥6 mg mL(-1). In the crude oils analysis, the DART(+)-Orbitrap mass spectra detected the typical N, NO, and O-containing compounds. In the paraffin samples, oxidized hydrocarbon species (Ox classes, where x = 1-4) with double-bond equivalent of 1-4 were detected, and their structures and connectivity were confirmed by collision-induced dissociation (CID) experiments. DART(+)-MS has identified the porphyrin standard compounds as [M + H](+) ions of m/z 615.2502 and 680.1763, where M = C44H30N4 and C44H28N4OV, respectively, based on the formula assignment and by phenyl losses observed on CID experiments.

CD19xCD3 DART protein mediates human B-cell depletion in vivo in humanized BLT mice

PubMed Central

Tsai, Perry; Thayer, William O; Liu, Liqin; Silvestri, Guido; Nordstrom, Jeffrey L; Garcia, J Victor

2016-01-01

Novel therapeutic strategies are needed for the treatment of hematologic malignancies; and bispecific antibody-derived molecules, such as dual-affinity re-targeting (DART) proteins, are being developed to redirect T cells to kill target cells expressing tumor or viral antigens. Here we present our findings of specific and systemic human B-cell depletion by a CD19xCD3 DART protein in humanized BLT mice. Administration of the CD19xCD3 DART protein resulted in a dramatic sustained depletion of human CD19+ B cells from the peripheral blood, as well as a dramatic systemic reduction of human CD19+ B-cell levels in all tissues (bone marrow, spleen, liver, lung) analyzed. When human CD8+ T cells were depleted from the mice, no significant B-cell depletion was observed in response to CD19xCD3 DART protein treatment, confirming that human CD8+ T cells are the primary effector cells in this in vivo model. These studies validate the use of BLT humanized mice for the in vivo evaluation and preclinical development of bispecific molecules that redirect human T cells to selectively deplete target cells. PMID:27119115

The Asteroid Impact Mission

NASA Astrophysics Data System (ADS)

Carnelli, Ian; Galvez, Andres; Mellab, Karim

2016-04-01

The Asteroid Impact Mission (AIM) is a small and innovative mission of opportunity, currently under study at ESA, intending to demonstrate new technologies for future deep-space missions while addressing planetary defense objectives and performing for the first time detailed investigations of a binary asteroid system. It leverages on a unique opportunity provided by asteroid 65803 Didymos, set for an Earth close-encounter in October 2022, to achieve a fast mission return in only two years after launch in October/November 2020. AIM is also ESA's contribution to an international cooperation between ESA and NASA called Asteroid Impact Deflection Assessment (AIDA), consisting of two mission elements: the NASA Double Asteroid Redirection Test (DART) mission and the AIM rendezvous spacecraft. The primary goals of AIDA are to test our ability to perform a spacecraft impact on a near-Earth asteroid and to measure and characterize the deflection caused by the impact. The two mission components of AIDA, DART and AIM, are each independently valuable but when combined they provide a greatly increased scientific return. The DART hypervelocity impact on the secondary asteroid will alter the binary orbit period, which will also be measured by means of lightcurves observations from Earth-based telescopes. AIM instead will perform before and after detailed characterization shedding light on the dependence of the momentum transfer on the asteroid's bulk density, porosity, surface and internal properties. AIM will gather data describing the fragmentation and restructuring processes as well as the ejection of material, and relate them to parameters that can only be available from ground-based observations. Collisional events are of great importance in the formation and evolution of planetary systems, own Solar System and planetary rings. The AIDA scenario will provide a unique opportunity to observe a collision event directly in space, and simultaneously from ground-based optical and radar facilities. For the first time, an impact experiment at asteroid scale will be performed with accurate knowledge of the precise impact conditions and also the impact outcome, together with information on the physical properties of the target, ultimately validating at appropriate scales our knowledge of the process and impact simulations. AIM's important technology demonstration component includes a deep-space optical communication terminal and inter-satellite network with two CubeSats deployed in the vicinity of the Didymos system and a lander on the surface of the secondary. To achieve a low-cost objective AIM's technology and scientific payload are being combined to support both close-proximity navigation and scientific investigations. AIM will demonstrate the capability to achieve a small spacecraft design with a very large technological and scientific mission return.

A method for rapid sampling and characterization of smokeless powder using sorbent-coated wire mesh and direct analysis in real time - mass spectrometry (DART-MS).

PubMed

Li, Frederick; Tice, Joseph; Musselman, Brian D; Hall, Adam B

2016-09-01

Improvised explosive devices (IEDs) are often used by terrorists and criminals to create public panic and destruction, necessitating rapid investigative information. However, backlogs in many forensic laboratories resulting in part from time-consuming GC-MS and LC-MS techniques prevent prompt analytical information. Direct analysis in real time - mass spectrometry (DART-MS) is a promising analytical technique that can address this challenge in the forensic science community by permitting rapid trace analysis of energetic materials. Therefore, we have designed a qualitative analytical approach that utilizes novel sorbent-coated wire mesh and dynamic headspace concentration to permit the generation of information rich chemical attribute signatures (CAS) for trace energetic materials in smokeless powder with DART-MS. Sorbent-coated wire mesh improves the overall efficiency of capturing trace energetic materials in comparison to swabbing or vacuuming. Hodgdon Lil' Gun smokeless powder was used to optimize the dynamic headspace parameters. This method was compared to traditional GC-MS methods and validated using the NIST RM 8107 smokeless powder reference standard. Additives and energetic materials, notably nitroglycerin, were rapidly and efficiently captured by the Carbopack X wire mesh, followed by detection and identification using DART-MS. This approach has demonstrated the capability of generating comparable results with significantly reduced analysis time in comparison to GC-MS. All targeted components that can be detected by GC-MS were detected by DART-MS in less than a minute. Furthermore, DART-MS offers the advantage of detecting targeted analytes that are not amenable to GC-MS. The speed and efficiency associated with both the sample collection technique and DART-MS demonstrate an attractive and viable potential alternative to conventional techniques. Copyright © 2016 The Chartered Society of Forensic Sciences. Published by Elsevier Ireland Ltd. All rights reserved.

Correlated observations of three triggered lightning flashes

NASA Technical Reports Server (NTRS)

Idone, V. P.; Orville, R. E.; Hubert, P.; Barret, L.; Eybert-Berard, A.

1984-01-01

Three triggered lightning flashes, initiated during the Thunderstorm Research International Program (1981) at Langmuir Laboratory, New Mexico, are examined on the basis of three-dimensional return stroke propagation speeds and peak currents. Nonlinear relationships result between return stroke propagation speed and stroke peak current for 56 strokes, and between return stroke propagation speed and dart leader propagation speed for 32 strokes. Calculated linear correlation coefficients include dart leader propagation speed and ensuing return stroke peak current (32 strokes; r = 0.84); and stroke peak current and interstroke interval (69 strokes; r = 0.57). Earlier natural lightning data do not concur with the weak positive correlation between dart leader propagation speed and interstroke interval. Therefore, application of triggered lightning results to natural lightning phenomena must be made with certain caveats. Mean values are included for the three-dimensional return stroke propagation speed and for the three-dimensional dart leader propagation speed.

Estimating the probability that the Taser directly causes human ventricular fibrillation.

PubMed

Sun, H; Haemmerich, D; Rahko, P S; Webster, J G

2010-04-01

This paper describes the first methodology and results for estimating the order of probability for Tasers directly causing human ventricular fibrillation (VF). The probability of an X26 Taser causing human VF was estimated using: (1) current density near the human heart estimated by using 3D finite-element (FE) models; (2) prior data of the maximum dart-to-heart distances that caused VF in pigs; (3) minimum skin-to-heart distances measured in erect humans by echocardiography; and (4) dart landing distribution estimated from police reports. The estimated mean probability of human VF was 0.001 for data from a pig having a chest wall resected to the ribs and 0.000006 for data from a pig with no resection when inserting a blunt probe. The VF probability for a given dart location decreased with the dart-to-heart horizontal distance (radius) on the skin surface.

ADART: an adaptive algebraic reconstruction algorithm for discrete tomography.

PubMed

Maestre-Deusto, F Javier; Scavello, Giovanni; Pizarro, Joaquín; Galindo, Pedro L

2011-08-01

In this paper we suggest an algorithm based on the Discrete Algebraic Reconstruction Technique (DART) which is capable of computing high quality reconstructions from substantially fewer projections than required for conventional continuous tomography. Adaptive DART (ADART) goes a step further than DART on the reduction of the number of unknowns of the associated linear system achieving a significant reduction in the pixel error rate of reconstructed objects. The proposed methodology automatically adapts the border definition criterion at each iteration, resulting in a reduction of the number of pixels belonging to the border, and consequently of the number of unknowns in the general algebraic reconstruction linear system to be solved, being this reduction specially important at the final stage of the iterative process. Experimental results show that reconstruction errors are considerably reduced using ADART when compared to original DART, both in clean and noisy environments.

Direct Analysis in Real Time-Mass Spectrometry for the Rapid Detection of Metabolites of Aconite Alkaloids in Intestinal Bacteria

NASA Astrophysics Data System (ADS)

Li, Xue; Hou, Guangyue; Xing, Junpeng; Song, Fengrui; Liu, Zhiqiang; Liu, Shuying

2014-12-01

In the present work, direct analysis of real time ionization combined with multi-stage tandem mass spectrometry (DART-MSn) was used to investigate the metabolic profile of aconite alkaloids in rat intestinal bacteria. A total of 36 metabolites from three aconite alkaloids were identified by using DART-MSn, and the feasibility of quantitative analysis of these analytes was examined. Key parameters of the DART ion source, such as helium gas temperature and pressure, the source-to-MS distance, and the speed of the autosampler, were optimized to achieve high sensitivity, enhance reproducibility, and reduce the occurrence of fragmentation. The instrument analysis time for one sample can be less than 10 s for this method. Compared with ESI-MS and UPLC-MS, the DART-MS is more efficient for directly detecting metabolic samples, and has the advantage of being a simple, high-speed, high-throughput method.

Direct analysis in real time-mass spectrometry for the rapid detection of metabolites of aconite alkaloids in intestinal bacteria.

PubMed

Li, Xue; Hou, Guangyue; Xing, Junpeng; Song, Fengrui; Liu, Zhiqiang; Liu, Shuying

2014-12-01

In the present work, direct analysis of real time ionization combined with multi-stage tandem mass spectrometry (DART-MS(n)) was used to investigate the metabolic profile of aconite alkaloids in rat intestinal bacteria. A total of 36 metabolites from three aconite alkaloids were identified by using DART-MS(n), and the feasibility of quantitative analysis of these analytes was examined. Key parameters of the DART ion source, such as helium gas temperature and pressure, the source-to-MS distance, and the speed of the autosampler, were optimized to achieve high sensitivity, enhance reproducibility, and reduce the occurrence of fragmentation. The instrument analysis time for one sample can be less than 10 s for this method. Compared with ESI-MS and UPLC-MS, the DART-MS is more efficient for directly detecting metabolic samples, and has the advantage of being a simple, high-speed, high-throughput method.

The DART dispersion analysis research tool: A mechanistic model for predicting fission-product-induced swelling of aluminum dispersion fuels. User`s guide for mainframe, workstation, and personal computer applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rest, J.

1995-08-01

This report describes the primary physical models that form the basis of the DART mechanistic computer model for calculating fission-product-induced swelling of aluminum dispersion fuels; the calculated results are compared with test data. In addition, DART calculates irradiation-induced changes in the thermal conductivity of the dispersion fuel, as well as fuel restructuring due to aluminum fuel reaction, amorphization, and recrystallization. Input instructions for execution on mainframe, workstation, and personal computers are provided, as is a description of DART output. The theory of fission gas behavior and its effect on fuel swelling is discussed. The behavior of these fission products inmore » both crystalline and amorphous fuel and in the presence of irradiation-induced recrystallization and crystalline-to-amorphous-phase change phenomena is presented, as are models for these irradiation-induced processes.« less

Direct Analysis in Real Time (DART) of an Organothiophosphate at Ultrahigh Resolution by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Tandem Mass Spectrometry

PubMed Central

Prokai, Laszlo; Stevens, Stanley M.

2016-01-01

Direct analysis in real time (DART) is a recently developed ambient ionization technique for mass spectrometry to enable rapid and sensitive analyses with little or no sample preparation. After swab-based field sampling, the organothiophosphate malathion was analyzed using DART-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Mass resolution was documented to be over 800,000 in full-scan MS mode and over 1,000,000 for an MS/MS product ion produced by collision-induced dissociation of the protonated analyte. Mass measurement accuracy below 1 ppm was obtained for all DART-generated ions that belonged to the test compound in the mass spectra acquired using only external mass calibration. This high mass measurement accuracy, achievable at present only through FTMS, was required for unequivocal identification of the corresponding molecular formulae. PMID:26784186

Direct Analysis in Real Time (DART) of an Organothiophosphate at Ultrahigh Resolution by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Tandem Mass Spectrometry.

PubMed

Prokai, Laszlo; Stevens, Stanley M

2016-01-16

Direct analysis in real time (DART) is a recently developed ambient ionization technique for mass spectrometry to enable rapid and sensitive analyses with little or no sample preparation. After swab-based field sampling, the organothiophosphate malathion was analyzed using DART-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Mass resolution was documented to be over 800,000 in full-scan MS mode and over 1,000,000 for an MS/MS product ion produced by collision-induced dissociation of the protonated analyte. Mass measurement accuracy below 1 ppm was obtained for all DART-generated ions that belonged to the test compound in the mass spectra acquired using only external mass calibration. This high mass measurement accuracy, achievable at present only through FTMS, was required for unequivocal identification of the corresponding molecular formulae.

Causal Cognition, Force Dynamics and Early Hunting Technologies

PubMed Central

Gärdenfors, Peter; Lombard, Marlize

2018-01-01

With this contribution we analyze ancient hunting technologies as one way to explore the development of causal cognition in the hominin lineage. Building on earlier work, we separate seven grades of causal thinking. By looking at variations in force dynamics as a central element in causal cognition, we analyze the thinking required for different hunting technologies such as stabbing spears, throwing spears, launching atlatl darts, shooting arrows with a bow, and the use of poisoned arrows. Our interpretation demonstrates that there is an interplay between the extension of human body through technology and expanding our cognitive abilities to reason about causes. It adds content and dimension to the trend of including embodied cognition in evolutionary studies and in the interpretation of the archeological record. Our method could explain variation in technology sets between archaic and modern human groups. PMID:29483885

Constraints on the perturbed mutual motion in Didymos due to impact-induced deformation of its primary after the DART impact

NASA Astrophysics Data System (ADS)

Hirabayashi, Masatoshi; Schwartz, Stephen R.; Yu, Yang; Davis, Alex B.; Chesley, Steven R.; Fahnestock, Eugene G.; Michel, Patrick; Richardson, Derek C.; Naidu, Shantanu P.; Scheeres, Daniel J.; Cheng, Andrew F.; Rivkin, Andrew S.; Benner, Lance A. M.

2017-12-01

Binary near-Earth asteroid (65803) Didymos is the target of the proposed NASA Double Asteroid Redirection Test (DART), part of the Asteroid Impact & Deflection Assessment (AIDA) mission concept. In this mission, the DART spacecraft is planned to impact the secondary body of Didymos, perturbing mutual dynamics of the system. The primary body is currently rotating at a spin period close to the spin barrier of asteroids, and materials ejected from the secondary due to the DART impact are likely to reach the primary. These conditions may cause the primary to reshape, due to landslides or internal deformation, changing the permanent gravity field. Here, we propose that if shape deformation of the primary occurs, the mutual orbit of the system would be perturbed due to a change in the gravity field. We use a numerical simulation technique based on the full two-body problem to investigate the shape effect on the mutual dynamics in Didymos after the DART impact. The results show that under constant volume, shape deformation induces strong perturbation in the mutual motion. We find that the deformation process always causes the orbital period of the system to become shorter. If surface layers with a thickness greater than ∼0.4 m on the poles of the primary move down to the equatorial region due to the DART impact, a change in the orbital period of the system and in the spin period of the primary will be detected by ground-based measurement.

DART-MS: A New Analytical Technique for Forensic Paint Analysis.

PubMed

Marić, Mark; Marano, James; Cody, Robert B; Bridge, Candice

2018-06-05

Automotive paint evidence is one of the most significant forms of evidence obtained in automotive-related incidents. Therefore, the analysis of automotive paint evidence is imperative in forensic casework. Most analytical schemes for automotive paint characterization involve optical microscopy, followed by infrared spectroscopy and pyrolysis-gas chromatography mass spectrometry ( py-GCMS) if required. The main drawback with py-GCMS, aside from its destructive nature, is that this technique is relatively time intensive in comparison to other techniques. Direct analysis in real-time-time-of-flight mass spectrometry (DART-TOFMS) may provide an alternative to py-GCMS, as the rapidity of analysis and minimal sample preparation affords a significant advantage. In this study, automotive clear coats from four vehicles were characterized by DART-TOFMS and a standard py-GCMS protocol. Principal component analysis was utilized to interpret the resultant data and suggested the two techniques provided analogous sample discrimination. Moreover, in some instances DART-TOFMS was able to identify components not observed by py-GCMS and vice versa, which indicates that the two techniques may provide complementary information. Additionally, a thermal desorption/pyrolysis DART-TOFMS methodology was also evaluated to characterize the intact paint chips from the vehicles to ascertain if the linear temperature gradient provided additional discriminatory information. All the paint samples were able to be discriminated based on the distinctive thermal desorption plots afforded from this technique, which may also be utilized for sample discrimination. On the basis of the results, DART-TOFMS may provide an additional tool to the forensic paint examiner.

Simulation of the dusty plasma environment of 65803 Didymos for the Asteroid Impact Mission (AIM)

NASA Astrophysics Data System (ADS)

Cipriani, Fabrice; Rodgers, David; Hilgers, Alain; Hess, Sebastien; Carnelli, Ian

2016-10-01

The Asteroid Impact and Deflection Assessment mission (AIDA) is a joint European-US technology demonstrator mission including the DART asteroid impactor (NASA/JHU/APL) and the AIM asteroid rendezvous platform (ESA/DLR/OCA) set to reach Near Earth binary Object 65803 Didymos in October 2022. Besides technology demonstration in the deep space communications domain and the realization of a kinetic impact on the moonlet to study deflection parameters, this asteroid rendezvous mission is an opportunity to carry out in-situ observations of the close environment of a binary system, addressing some fundamental science questions. The MASCOT-2 lander will be released from the AIM platform and operate at the surface of the moonlet of 65803 Didymos, complemented by the ability of the Cubesat Opportunity Payloads (COPINS) to sample the close environment of the binary.In this context, we have developed an model describing the plasma and charged dust components of the near surface environment of the moonlet (170m in diameter), targeted by the MASCOT-2 lander and of the DART impactor. We performed numerical simulations in order to estimate the electrostatic surface potentials at various locations of the surface, resulting from its interaction with the solar wind plasma and solar photons. In addition, we describe charging levels, density profiles, and velocity distribution of regolith grains lifted out from the surface up to about 70m above the surface.

NASA DART

NASA Technical Reports Server (NTRS)

Hale, L. C.

1977-01-01

A low cost conductivity probe system for use with the Super Loki Dart meteorological sounding rocket is described. Miniaturization of the Super Arcas blunt probe, operation of the blunt probe experiment, flight tests, and development of a Lyman-alpha probe are among the topics discussed.

Design and test of a 2m x 4m DART system

NASA Technical Reports Server (NTRS)

Tolomeo, J.; Loane, J.; Cross, G.; Tenerelli, D.; Sable, B.; Decker, T.; Nelson, T.; Joseph, B.; Windes, C.; Kokawa, D.;

New Developments in the Data Assimilation Research Testbed

NASA Astrophysics Data System (ADS)

Hoar, T. J.; Anderson, J. L.; Raeder, K.; Karspeck, A. R.; Romine, G.; Liu, H.; Collins, N.

2011-12-01

NCAR's Data Assimilation Research Testbed (DART) is a community facility that provides ensemble data assimilation tools for geophysical applications. DART works with an expanding set of models and a wide range of conventional and novel observations, and provides a variety of assimilation algorithms and diagnostic tools. The Kodiak release of DART became available in July 2011 and includes more than 20 major feature enhancements, support for 24 models, support for (at least) 14 observation formats, expanded documentation and diagnostic tools, and 12 new utilities. A few examples of research projects that demonstrate the effectiveness and flexibility of the DART are described. The Community Atmosphere Model (CAM) and DART assimilated all the observations that were used in the NCEP/NCAR Reanalysis to produce a global, 6-hourly, 80-member ensemble reanalysis for 1998 through the present. The dataset is ideal for research applications that would benefit from an ensemble of equally-likely atmospheric states that are consistent with observations. Individual ensemble members may be used as a "data atmosphere" in any Community Earth System Model (CESM) experiment. The CESM interfaces for the Parallel Ocean Program (POP) and the Community Land Model (CLM) also support multiple instances, allowing data assimilation experiments exploiting unique atmospheric forcing for each POP or CLM model instance. A multi-year DART ocean assimilation has been completed and provides valuable insight into the successes and challenges of oceanic data assimilation. The DART/CLM research focuses on snow cover fraction and snow depth. The Weather Research and Forecasting (WRF) model was used with DART to perform a real-time CONUS domain mesoscale ensemble analysis with continuous cycling for 47 days. A member was selected once daily for high-resolution convective forecasts supporting a test phase of the Deep Convective Clouds and Chemistry experiment and the Storm Prediction Center spring experiment. The impacts of Moderate Resolution Imaging Spectroradiometer (MODIS) infrared and Advanced Microwave Scanning Radiometer (AMSR) microwave total precipitable water (TPW) observations on analyses and forecasts of tropical cyclone Sinlaku (2008) are investigated by performing assimilations with a 45km resolution WRF model over the Western Pacific domain for 8-14 Septmber, 2008. Particular emphasis is on the performance of the assimilation algorithms in the hurricane core and the impact of novel observations in the hurricane core.

Wavefront Sensing and Control Technology for Submillimeter and Far-Infrared Space Telescopes

NASA Technical Reports Server (NTRS)

Redding, Dave

2004-01-01

The NGST wavefront sensing and control system will be developed to TRL6 over the next few years, including testing in a cryogenic vacuum environment with traceable hardware. Doing this in the far-infrared and submillimeter is probably easier, as some aspects of the problem scale with wavelength, and the telescope is likely to have a more stable environment; however, detectors may present small complications. Since this is a new system approach, it warrants a new look. For instance, a large space telescope based on the DART membrane mirror design requires a new actuation approach. Other mirror and actuation technologies may prove useful as well.

Deep Aquifer Remediation Tools (DARTs): A new technology for ground-water remediation

USGS Publications Warehouse

Naftz, David L.; Davis, James A.

1999-01-01

Potable ground-water supplies throughout the world are contaminated or threatened by advancing plumes containing radionuclides, metals, and organic compounds. Currently (1999), the most widely used method of ground-water remediation is a combination of extraction, ex-situ treatment, and discharge of the treated water, commonly known as pump and treat. Pump-and-treat methods are costly and often ineffective in meeting long-term protection standards (Travis and Doty, 1990; Gillham and Burris, 1992; National Research Council, 1994). This fact sheet describes a new and potentially cost-effective technology for removal of organic and inorganic contaminants from ground water. The U.S. Geological Survey (USGS) is currently exploring the possibilities of obtaining a U.S. Patent for this technology.

Morphological comparison of five species of poison dart frogs of the genus Ranitomeya (Anura: Dendrobatidae) including the skeleton, the muscle system and inner organs.

PubMed

Krings, Markus; Klein, Benjamin; Heneka, Markus J; Rödder, Dennis

2017-01-01

The morphology of larvae stages of most amphibians are often completely different than in adults. Tadpole descriptions have historically been based on external characters like morphometrics, color pattern and oral disc structure. Other papers described anatomical details by the use of dissections. The increase in micro-CT scanning technology provides an opportunity to quantify and describe in detail internal characters like skeleton, musculature and organs. To date, no such tadpole descriptions exist for the well-studied Neotropical poison dart frog genus Ranitomeya (Anura: Dendrobatidae). Here we provide descriptions of the internal skeletal, musculature and organ structures of five Ranitomeya species and then provide morphological comparisons. Contrary to previous observations, closely related species display several morphological differences. For example, we observed considerable variation in chondrocranial characters, the extent of cranial ossifications, the appearance of some cranial muscles and the arrangement of inner organs. Further studies on the tadpole morphology of more species of Ranitomeya and other dendrobatid genera are needed to enable us to understand the complete morphological variation in this group.

Morphological comparison of five species of poison dart frogs of the genus Ranitomeya (Anura: Dendrobatidae) including the skeleton, the muscle system and inner organs

PubMed Central

Krings, Markus; Klein, Benjamin; Heneka, Markus J.

2017-01-01

The morphology of larvae stages of most amphibians are often completely different than in adults. Tadpole descriptions have historically been based on external characters like morphometrics, color pattern and oral disc structure. Other papers described anatomical details by the use of dissections. The increase in micro-CT scanning technology provides an opportunity to quantify and describe in detail internal characters like skeleton, musculature and organs. To date, no such tadpole descriptions exist for the well-studied Neotropical poison dart frog genus Ranitomeya (Anura: Dendrobatidae). Here we provide descriptions of the internal skeletal, musculature and organ structures of five Ranitomeya species and then provide morphological comparisons. Contrary to previous observations, closely related species display several morphological differences. For example, we observed considerable variation in chondrocranial characters, the extent of cranial ossifications, the appearance of some cranial muscles and the arrangement of inner organs. Further studies on the tadpole morphology of more species of Ranitomeya and other dendrobatid genera are needed to enable us to understand the complete morphological variation in this group. PMID:28235032

Shaping of parabolic cylindrical membrane reflectors for the DART precision test bed

NASA Technical Reports Server (NTRS)

White, C.; Salama, M.; Dragovan, M.; Schroeder, J.; Barber, D.; Dooley, J.

2003-01-01

The DART is a new telescope architecture consisting of two cylindrical parabolic reflectors. The system is ideally suited to using tensioned membranes for the reflective surfaces, owing to the zero Gaussian curvature of a cylindrical parabola.

The feasibility of implementing the data analysis and reporting techniques (DART) package in Virginia.

DOT National Transportation Integrated Search

1980-01-01

This project was undertaken for the Virginia Department of Transportation Safety to assess the feasibility of implementing the Data Analysis and Reporting Techniques (DART) computer software system in Virginia. Following a review of available literat...

DART Fixed-Route Service Review Study: Executive Summary

DOT National Transportation Integrated Search

1995-02-01

This study analyzes the probable impact of new light rail transit and commuter rail service into the Dallas Central Business District on existing Dallas Area Rapid Transit (DART) bus service. It looks at possible route and schedule modifications to e...

Program Processes Thermocouple Readings

NASA Technical Reports Server (NTRS)

Quave, Christine A.; Nail, William, III

1995-01-01

Digital Signal Processor for Thermocouples (DART) computer program implements precise and fast method of converting voltage to temperature for large-temperature-range thermocouple applications. Written using LabVIEW software. DART available only as object code for use on Macintosh II FX or higher-series computers running System 7.0 or later and IBM PC-series and compatible computers running Microsoft Windows 3.1. Macintosh version of DART (SSC-00032) requires LabVIEW 2.2.1 or 3.0 for execution. IBM PC version (SSC-00031) requires LabVIEW 3.0 for Windows 3.1. LabVIEW software product of National Instruments and not included with program.

Post Deflection Impact Risk Analysis of the Double Asteroid Redirection Test (DART)

NASA Astrophysics Data System (ADS)

Eggl, S.; Hestroffer, D.

2017-09-01

Collisions between potentially hazardous near-Earth objects and our planet are among the few natural disasters that can be avoided by human intervention. The complexity of such an endeavor necessitates an asteroid orbit deflection test mission, however, ensuring all relevant knowledge is present when an asteroid on a collision course with the Earth is indeed discovered. The double asteroid redirection test (DART) mission concept currently investigated by NASA would serve such a purpose. The aim of our research is to make certain that DART does not turn a previously harmless asteroid into a potentially dangerous one.

DART model for irradiation-induced swelling of dispersion fuel elements including aluminum-fuel interaction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rest, J.; Hofman, G.L.

1997-12-01

The Dispersion Analysis Research Tool (DART) contains models for fission-gas-induced fuel swelling, interaction of fuel with the matrix aluminum, for the resultant reaction-product swelling, and for the calculation of the stress gradient within the fuel particle. The effects of an aluminide shell on fuel particle swelling are evaluated. Validation of the model is demonstrated by a comparison of DART calculations of fuel swelling of U{sub 3}SiAl-Al and U{sub 3}Si{sub 2}-Al for various dispersion fuel element designs with the data.

DART model for irradiation-induced swelling of uranium silicide dispersion fuel elements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rest, J.; Hofman, G.L.

1999-04-01

Models for the interaction of uranium silicide dispersion fuels with an aluminum matrix, for the resultant reaction product swelling, and for the calculation of the stress gradient within the fuel particles are described within the context of DART fission-gas-induced swelling models. The effects of an aluminide shell on fuel particle swelling are evaluated. Validation of the model is demonstrated by comparing DART calculations with irradiation data for the swelling of U{sub 3}SiAl-Al and U{sub 3}Si{sub 2}-Al in variously designed dispersion fuel elements.

Dallas area rapid transit LRT starter line assessment study design. Final research report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shunk, G.A.; Turnbull, K.F.; Lindquist, N.F.

1995-03-01

Light rail transit (LRT) systems have recently been implemented in a number of urban areas throughout the United States and additional projects are in various stages of planning and development. Questions have been raised concerning the impact of these systems on ridership levels, transit operating costs, regional mobility, land use, economic development, energy, air quality, congestion levels, and other factors. The implementation of the Dallas Area Rapid Transit (DART) LRT starter line provides the opportunity to assess the impact of an LRT system in a Southwestern city in the United States. This research project was undertaken to assist with themore » development of a comprehensive study design for assessing the effects of the DART LRT starter line. To accomplish this objective, a review was conducted of before-and-after studies of recent LRT, heavy rail, and high-occupancy vehicle (HOV) projects. The goals and objectives of the DART system were also reviewed and existing transportation-related data collection activities in the Dallas area were examined. This information was used to develop a preliminary study design for assessing the effects of the DART LRT starter line. This report documents the review of recent before-and-after studies and presents the preliminary study design for assessing the effects of the DART LRT starter line.« less

Rapid qualitative analysis of 2 flavonoids, rutin and silybin, in medical pills by direct analysis in real-time mass spectrometry (DART-MS) combined with in situ derivatization.

PubMed

Nagy, Tibor; Kuki, Ákos; Nagy, Lajos; Zsuga, Miklós; Kéki, Sándor

2018-03-01

Direct analysis in real-time mass spectrometry (DART-MS) with in situ silylation was used for the rapid analysis of the flavonoids silybin ((2R,3R)-3,5,7-trihydroxy-2-[3-(4-hydroxy-3-methoxyphenyl)-2-hydroxymethyl-2,3-dihydrobenzo[1,4]dioxin-6-yl]chroman-4-one) and rutin (quercetin-3-O-rutinoside). Three different derivatization reagents, hexamethyldisilazane/trimethylchlorosilane/pyridine (HMDS/TMCS/pyridine), N,O-bis(trimethylsilyl)acetamide/trimethylchlorosilane/N-trimethylsilyimidazole (BSA/TMCS/TMSI), and N,O-bis(trimethylsilyl)trifluoroacetamide/trimethylchlorosilane (BSTFA/TMCS), were applied. Silybin and rutin were detected with various degrees of silylation, and the formation of dimers with pyridine and imidazole was also observed. HMDS/TMCS/pyridine was the best choice for the DART-MS analysis of silybin, and BSA/TMCS/TMSI was the most effective for the detection of rutin. The effects of the DART source temperature on desorption, ionization, in-source fragmentation, dimer formation, and hydrolysis of the trimethylsilyl groups were also studied. In addition, the collision-induced dissociation properties of the derivatized silybin and rutin were explored. With our in situ silylation method, the derivatized bioactive compounds in intact medical pills could also be detected by DART-MS. Copyright © 2017 John Wiley & Sons, Ltd.

Targeting CD123 in acute myeloid leukemia using a T-cell–directed dual-affinity retargeting platform

PubMed Central

Al-Hussaini, Muneera; Rettig, Michael P.; Ritchey, Julie K.; Karpova, Darja; Uy, Geoffrey L.; Eissenberg, Linda G.; Gao, Feng; Eades, William C.; Bonvini, Ezio; Chichili, Gurunadh R.; Moore, Paul A.; Johnson, Syd; Collins, Lynne

2016-01-01

T-cell–directed killing of tumor cells using bispecific antibodies is a promising approach for the treatment of hematologic malignancies. Here we describe our preclinical work with a dual-affinity retargeting (DART) molecule generated from antibodies to CD3 and CD123, designed to redirect T cells against acute myeloid leukemia blasts. The CD3×CD123 DART (also referred to as MGD006/S80880) consists of 2 independent polypeptides, each composed of the VH of 1 antibody in tandem with the VL of the other antibody. The target antigen CD123 (interleukin 3RA) is highly and differentially expressed in acute myeloid leukemia (AML) blasts compared with normal hematopoietic stem and progenitor cells. In this study we demonstrate that the CD3×CD123 DART binds to both human CD3 and CD123 to mediate target-effector cell association, T-cell activation, proliferation, and receptor diversification. The CD3×CD123 DART also induces a dose-dependent killing of AML cell lines and primary AML blasts in vitro and in vivo. These results provide the basis for testing the CD3×CD123 DART in the treatment of patients with CD123+ AML. PMID:26531164

Incomplete projection reconstruction of computed tomography based on the modified discrete algebraic reconstruction technique

NASA Astrophysics Data System (ADS)

Yang, Fuqiang; Zhang, Dinghua; Huang, Kuidong; Gao, Zongzhao; Yang, YaFei

2018-02-01

Based on the discrete algebraic reconstruction technique (DART), this study aims to address and test a new improved algorithm applied to incomplete projection data to generate a high quality reconstruction image by reducing the artifacts and noise in computed tomography. For the incomplete projections, an augmented Lagrangian based on compressed sensing is first used in the initial reconstruction for segmentation of the DART to get higher contrast graphics for boundary and non-boundary pixels. Then, the block matching 3D filtering operator was used to suppress the noise and to improve the gray distribution of the reconstructed image. Finally, simulation studies on the polychromatic spectrum were performed to test the performance of the new algorithm. Study results show a significant improvement in the signal-to-noise ratios (SNRs) and average gradients (AGs) of the images reconstructed from incomplete data. The SNRs and AGs of the new images reconstructed by DART-ALBM were on average 30%-40% and 10% higher than the images reconstructed by DART algorithms. Since the improved DART-ALBM algorithm has a better robustness to limited-view reconstruction, which not only makes the edge of the image clear but also makes the gray distribution of non-boundary pixels better, it has the potential to improve image quality from incomplete projections or sparse projections.

Rapid Analysis of Ingredients in Cream Using Ultrasonic Mist-Direct Analysis in Real-Time Time-of-Flight Mass Spectrometry

NASA Astrophysics Data System (ADS)

Shimada, Haruo; Maeno, Katsuyuki; Kinoshita, Kazumasa; Shida, Yasuo

2017-07-01

A novel method for the simultaneous detection of ingredients in pharmaceutical applications such as creams and lotions was developed. An ultrasonic atomizer has been used to produce a mist containing ingredients. The analyte molecules in the mist can be ionized by using direct analysis in real time (DART) at lower temperature than traditionally used, and we thus solved the problem of normal DART-MS measurement using a high-temperature gas. Thereby, molecular-related ions of heat-unstable components and nonvolatile components became detectable. The deprotonated molecular ion of glycyrrhizic acid (m/z 821), which is unstable at high temperatures, was detected without pyrolysis by ultrasonic mist-DART-MS using unheated helium gas, although it was not detected by normal DART-MS using heated helium gas. The cationized molecular ions of derivatives of polyethylene glycol fatty acid monoesters, which are nonvolatile compounds, were also detected as m/z peaks observed from 800 to 2300. Although the protonated molecular ion of tocopherol acetate was not detected in ionization by ultrasonic mist, it was detected by ultrasonic mist-DART-MS even in the emulsion. It was not necessary to dissolve a sample completely to detect its ions. This method enabled us to obtain the composition of pharmaceutical applications simply and rapidly.

The contribution of coping related variables and cardiac vagal activity on the performance of a dart throwing task under pressure.

PubMed

Mosley, Emma; Laborde, Sylvain; Kavanagh, Emma

2017-10-01

The aims of this study were 1) to assess the predictive role of coping related variables (CRV) on cardiac vagal activity (derived from heart rate variability), and 2) to investigate the influence of CRV (including cardiac vagal activity) on a dart throwing task under low pressure (LP) and high pressure (HP) conditions. Participants (n=51) completed trait CRV questionnaires: Decision Specific Reinvestment Scale, Movement Specific Reinvestment Scale and Trait Emotional Intelligence Questionnaire. They competed in a dart throwing task under LP and HP conditions. Cardiac vagal activity measurements were taken at resting, task and during recovery for 5min. Self-reported ratings of stress were recorded at three time points via a visual analogue scale. Upon completion of the task, self-report measures of motivation, stress appraisal, attention, perceived pressure and dart throwing experience were completed. Results indicated that resting cardiac vagal activity had no predictors. Task cardiac vagal activity was predicted by resting cardiac vagal activity in both pressure conditions with the addition of a trait CRV in HP. Post task cardiac vagal activity was predicted by resting cardiac vagal activity in both conditions with the addition of a trait CRV in HP. Cardiac vagal reactivity (difference from resting to task) was predicted by a trait CRV in HP conditions. Cardiac vagal recovery (difference from task to post task) was predicted by a state CRV only in LP. Dart throwing task performance was predicted by a combination of both CRV and cardiac vagal activity. The current research suggests that coping related variables and cardiac vagal activity influence dart throwing task performance differently dependent on pressure condition. Copyright © 2017 Elsevier Inc. All rights reserved.

The NASA Lightning Nitrogen Oxides Model (LNOM): Recent Updates and Applications

NASA Technical Reports Server (NTRS)

Koshak, William; Peterson, Harold; Biazar, Arastoo; Khan, Maudood; Wang, Lihua; Park, Yee-Hun

2011-01-01

Improvements to the NASA Marshall Space Flight Center Lightning Nitrogen Oxides Model (LNOM) and its application to the Community Multiscale Air Quality (CMAQ) modeling system are presented. The LNOM analyzes Lightning Mapping Array (LMA) and National Lightning Detection Network(tm) (NLDN) data to estimate the raw (i.e., unmixed and otherwise environmentally unmodified) vertical profile of lightning NOx (= NO + NO2). Lightning channel length distributions and lightning 10-m segment altitude distributions are also provided. In addition to NOx production from lightning return strokes, the LNOM now includes non-return stroke lightning NOx production due to: hot core stepped and dart leaders, stepped leader corona sheath, K-changes, continuing currents, and M-components. The impact of including LNOM-estimates of lightning NOx for an August 2006 run of CMAQ is discussed.

Dynamic Shape Capture of Free-Swimming Aquatic Life using Multi-view Stereo

NASA Astrophysics Data System (ADS)

Daily, David

2017-11-01

The reconstruction and tracking of swimming fish in the past has either been restricted to flumes, small volumes, or sparse point tracking in large tanks. The purpose of this research is to use an array of cameras to automatically track 50-100 points on the surface of a fish using the multi-view stereo computer vision technique. The method is non-invasive thus allowing the fish to swim freely in a large volume and to perform more advanced maneuvers such as rolling, darting, stopping, and reversing which have not been studied. The techniques for obtaining and processing the 3D kinematics and maneuvers of tuna, sharks, stingrays, and other species will be presented and compared. The National Aquarium and the Naval Undersea Warfare Center and.

Analysis of writing inks on paper using direct analysis in real time mass spectrometry.

PubMed

Jones, Roger W; McClelland, John F

2013-09-10

Ink analysis is central to questioned document examination. We applied direct analysis in real time mass spectrometry (DART MS) to ballpoint, gel, and fluid writing ink analysis. DART MS acquires the mass spectrum of an ink while it is still on a document without altering the appearance of the document. Spectra were acquired from ink on a variety of papers, and the spectrum of the blank paper could be subtracted out to produce a cleanly isolated ink spectrum in most cases. Only certain heavy or heavily processed papers interfered. The time since an ink is written on paper has a large effect on its spectrum. DART spectra change radically during the first few months after an ink is written as the more volatile components evaporate, but the spectra stabilize after that. A library-search study involving 166 well-aged inks assessed the ability to identify inks from their DART spectra. The aggregate success rate was 92%. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

DART -- Data acquisition for the next generation of Fermilab fixed target experiments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oleynik, G.; Anderson, J.; Appleton, L.

1994-02-01

DART is the name of the data acquisition effort for Fermilab experiments taking data in the '94--'95 time frame and beyond. Its charge is to provide a common system of hardware and software, which can be easily configured and extended to meet the wide range of data acquisition requirements of the experiments. Its strategy is to provide incrementally functional data acquisition systems to the experiments at frequent intervals to support the ongoing DA activities of the experiments. DART is a collaborative development effort between the experimenters and the Fermilab Computing Division. Experiments collaborating in DART cover a range of requirementsmore » from 400 Kbytes/sec event readout using a single DA processor, to 200 Mbytes/sec event readout involving 10 parallel readout streams, 10 VME event building planes and greater than 1,000 MIPs of event filter processing. The authors describe the requirements, architecture, and plans for the project and report on its current status.« less

The era of 3Rs implementation in developmental and reproductive toxicity (DART) testing: Current overview and future perspectives.

PubMed

Beekhuijzen, Manon

2017-09-01

Since adoption of the first globally implemented guidelines for developmental and reproductive toxicity (DART) testing for pharmaceuticals, industrial chemicals and agrochemicals, many years passed without major updates. However in recent years, significant changes in these guidelines have been made or are being implemented. These changes have been guided by the ethical drive to reduce, refine and replace (3R) animal testing, as well as the addition of endocrine disruptor relevant endpoints. Recent applied improvements have focused on reduction and refinement. Ongoing scientific and technical innovations will provide the means for replacement of animal testing in the future and will improve predictivity in humans. The aim of this review is to provide an overview of ongoing global DART endeavors in respect to the 3Rs, with an outlook towards future advances in DART testing aspiring to reduce animal testing to a minimum and the supreme ambition towards animal-free hazard and risk assessment. Copyright © 2017 Elsevier Inc. All rights reserved.

The Double Asteroid Redirection Test (DART) for the AIDA Mission

NASA Astrophysics Data System (ADS)

Stickle, Angela; Cheng, Andy F.; Michel, Patrick; Barnouin, Olivier S.; Campo Bagatin, Adriano; Miller, Paul L.; Pravec, Petr; Richardson, Derek C.; Schwartz, Stephen R.; Tsiganis, Kleomenis; Ulamec, Stephan; AIDA Impact Modeling and Simulation Working Group

2016-10-01

The Asteroid Impact Deflection Assessment (AIDA) mission will be the first space experiment to demonstrate asteroid impact hazard mitigation using a kinetic impactor. AIDA is a joint ESA-NASA cooperative project, consisting of the NASA Double Asteroid Redirection Test (DART) mission, which provides the kinetic impactor, and the ESA Asteroid Impact Mission (AIM) rendezvous spacecraft. DART is a Phase A study supported by NASA, and AIM is a Phase B1 study supported by ESA. The AIDA target is the near-Earth binary asteroid 65803 Didymos, which will make a close approach to Earth in October, 2022. The DART spacecraft is designed to impact the Didymos secondary at ~6 km/s and deflect its trajectory, changing the orbital period of the binary. This change can be measured by Earth-based optical and radar observations. The primary goals of AIDA are to (1) perform a full-scale demonstration of asteroid deflection by kinetic impact; (2) measure the resulting deflection; and (3) validate and improve models for momentum transfer in high-speed impacts on an asteroid. The combined DART and AIM missions will provide the first measurements of momentum transfer efficiency from a kinetic impact at full scale on an asteroid, where the impact conditions of the projectile are known, and physical properties and internal structures of the target asteroid are also characterized. In addition to a predicted 4.4 minute change in the binary orbit period, assuming unit momentum transfer efficiency, the DART kinetic impact is predicted to induce forced librations of the Didymos secondary of possibly several degrees amplitude. Models predict the impact will create a 6-17 meter diameter crater, depending on target physical properties, and it will release a volume of particulate ejecta that may be directly observable from Earth or even resolvable as a coma or an ejecta tail by ground-based telescopes. Current simulations of the DART impact provide predictions for momentum transfer, crater size, and ejecta mass following impact. Additional work benchmarking impact hydrocodes with one another provides a way to bound the uncertainty in these critical simulations, allowing better predictions for the momentum transfer to the moon of Didymos.

On the effect specificity of accessory gland products transferred by the love-dart of land snails.

PubMed

Lodi, Monica; Koene, Joris M

2016-05-13

Sexual selection favours the evolution of male bioactive substances transferred during mating to enhance male reproductive success by affecting female physiology. These effects are mainly well documented for separate-sexed species. In simultaneous hermaphrodites, one of the most peculiar examples of transfer of such substances is via stabbing a so-called love-dart in land snails. This calcareous stylet delivers mucous products produced by accessory glands into the mate's haemolymph. In Cornu aspersum, this mucus temporarily causes two changes in the recipient. First, the spermatophore uptake into the spermatophore-receiving organ, called diverticulum, is probably favoured by contractions of this organ. Second, the amount of stored sperm increases by contractions of the copulatory canal, which close off the tract leading to the sperm digesting organ. However, it has yet to be determined whether these effects are similar across species, which would imply a common strategy of the dart in increasing male reproductive success. We performed a cross-reactivity test to compare the in vitro response of the diverticulum and copulatory canal of C. aspersum (Helicidae) to its own and other species' mucus (seven helicids and one bradybaenid). We found that the contractions in the diverticulum were only induced by dart mucus of certain species, while the copulatory canal responded equally to all but one species' mucus tested. In addition, we report a newly-discovered effect causing the shortening of the diverticulum, which is also only caused by dart mucus of certain species. The advantage seems to be a distance reduction to the sperm storage organ. All these findings are the first to shed light on the evolution of the different functions of accessory gland products in dart-bearing species. These functions may be achieved via common physiological changes caused by the substances contained in the dart mucus, since the responses evoked were similar across species' mucus. Moreover, while these substances can act similarly in separate-sexed species as in simultaneous hermaphrodites, differences may occur in their evolution between the two sexual systems.

Fermilab`s DART DA system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pordes, R.; Anderson, J.; Berg, D.

1994-04-01

DART is the new data acquisition system designed and implemented for six Fermilab experiments by the Fermilab Computing Division and the experiments themselves. The complexity of the experiments varies greatly. Their data taking throughput and event filtering requirements range from a few (2-5) to tens (80) of CAMAC, FASTBUS and home built front end crates; from a few 100 KByte/sec to 160 MByte/sec front end data collection rates; and from 0-3000 Mips of level 3 processing. The authors report on the architecture and implementation of DART to this date, and the hardware and software components that are being developed andmore » supported.« less

Fermilab`s DART DA system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pordes, R.; Anderson, J.; Berg, D.

1994-12-31

DART is the new data acquisition system designed and implemented for six Fermilab experiments by the Fermilab Computing Division and the experiments themselves. The complexity of the experiments varies greatly. Their data taking throughput and event filtering requirements range from a few (2-5) to tens (80) of CAMAC, FASTBUS and home built front end crates; from a few 100 KByte/sec to 160 MByte/sec front end data collection rates; and from 0-3000 Mips of level 3 processing. The authors report on the architecture and implementation of DART to this data, and the hardware and software components that are being developed andmore » supported.« less

Comparative mapping of quantitative trait loci associated with waterlogging tolerance in barley (Hordeum vulgare L.).

PubMed

Li, Haobing; Vaillancourt, René; Mendham, Neville; Zhou, Meixue

2008-08-27

Resistance to soil waterlogging stress is an important plant breeding objective in high rainfall or poorly drained areas across many countries in the world. The present study was conducted to identify quantitative trait loci (QTLs) associated with waterlogging tolerance (e.g. leaf chlorosis, plant survival and biomass reduction) in barley and compare the QTLs identified across two seasons and in two different populations using a composite map constructed with SSRs, RFLP and Diversity Array Technology (DArT) markers. Twenty QTLs for waterlogging tolerance related traits were found in the two barley double haploid (DH) populations. Several of these QTLs were validated through replication of experiments across seasons or by co-location across populations. Some of these QTLs affected multiple waterlogging tolerance related traits, for example, QTL Qwt4-1 contributed not only to reducing barley leaf chlorosis, but also increasing plant biomass under waterlogging stress, whereas other QTLs controlled both leaf chlorosis and plant survival. Improving waterlogging tolerance in barley is still at an early stage compared with other traits. QTLs identified in this study have made it possible to use marker assisted selection (MAS) in combination with traditional field selection to significantly enhance barley breeding for waterlogging tolerance. There may be some degree of homoeologous relationship between QTLs controlling barley waterlogging tolerance and that in other crops as discussed in this study.

16 CFR 1306.3 - Banned hazardous products.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Banned hazardous products. 1306.3 Section 1306.3 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.3 Banned hazardous products. Any lawn dart is a banned hazardous...

16 CFR 1306.3 - Banned hazardous products.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Banned hazardous products. 1306.3 Section 1306.3 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.3 Banned hazardous products. Any lawn dart is a banned hazardous...

16 CFR 1306.3 - Banned hazardous products.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Banned hazardous products. 1306.3 Section 1306.3 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.3 Banned hazardous products. Any lawn dart is a banned hazardous...

16 CFR 1306.3 - Banned hazardous products.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Banned hazardous products. 1306.3 Section 1306.3 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.3 Banned hazardous products. Any lawn dart is a banned hazardous...

18. View of north side of East Ward Street at ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

18. View of north side of East Ward Street at the intersection with North Dart Street, facing northwest. - Gaskin Avenue Neighborhood, Bounded by Dart Street to east, CSX Railroad to south, Pearl & Madison Avenues to west, & Wilson & Gordon Streets to north, Douglas, Coffee County, GA

Quantitative analysis of phosphoric acid esters in aqueous samples by isotope dilution stir-bar sorptive extraction combined with direct analysis in real time (DART)-Orbitrap mass spectrometry.

PubMed

Bridoux, Maxime C; Malandain, Hélène; Leprince, Françoise; Progent, Frédéric; Machuron-Mandard, Xavier

2015-04-15

A novel hyphenated technique, namely the combination of stir bar sorptive extraction (SBSE) with isotope dilution direct analysis in real time (DART) Orbitrap™ mass spectrometry (OT-MS) is presented for the extraction of phosphoric acid alkyl esters (tri- (TnBP), di- (HDBP), and mono-butyl phosphate (H2MBP)) from aqueous samples. First, SBSE of phosphate esters was performed using a Twister™ coated with 24 μL of polydimethylsiloxane (PDMS) as the extracting phase. SBSE was optimized for extraction pH, phase ratio (PDMS volume/aqueous phase volume), stirring speed, extraction time and temperature. Then, coupling of SBSE to DART/Orbitrap-MS was achieved by placing the Twister™ in the middle of an open-ended glass tube between the DART and the Orbitrap™. The DART mass spectrometric response of phosphate esters was probed using commercially available and synthesized alkyl phosphate ester standards. The positive ion full scan spectra of alkyl phosphate triesters (TnBP) was characterized by the product of self-protonation [M+H](+) and, during collision-induced dissociation (CID), the major fragmentation ions corresponded to consecutive loss of alkyl chains. Negative ionization gave abundant [M-H](-) ions for both HDnBP and H2MnBP. Twisters™ coated with PDMS successfully extracted phosphate acid esters (tri-, di- and mono-esters) granted that the analytes are present in the aqueous solution in the neutral form. SBSE/DART/Orbitrap-MS results show a good linearity between the concentrations and relative peak areas for the analytes in the concentration range studied (0.1-750 ng mL(-1)). Reproducibility of this SBSE/DART/Orbitrap-MS method was evaluated in terms of %RSD by extracting a sample of water fortified with the analytes. The %RSDs for TnBP, HDnBP and H2MnBP were 4, 3 and 3% (n=5) using the respective perdeuterated internal standards. Matrix effects were investigated by matrix matched calibration standards using underground water samples (UWS) and river water samples (RWS). Matrix effects were effectively compensated by the addition of the perdeuterated internal standards. The application of this new SBSE/DART/Orbitrap-MS method should be very valuable for on-site sampling/monitoring, limiting the transport of large volumes of water samples from the sampling site to the laboratory. Copyright © 2015 Elsevier B.V. All rights reserved.

A feasibility study of dynamic adaptive radiotherapy for nonsmall cell lung cancer

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Minsun, E-mail: mk688@uw.edu; Phillips, Mark H.

2016-05-15

Purpose: The final state of the tumor at the end of a radiotherapy course is dependent on the doses given in each fraction during the treatment course. This study investigates the feasibility of using dynamic adaptive radiotherapy (DART) in treating lung cancers assuming CBCT is available to observe midtreatment tumor states. DART adapts treatment plans using a dynamic programming technique to consider the expected changes of the tumor in the optimization process. Methods: DART is constructed using a stochastic control formalism framework. It minimizes the total expected number of tumor cells at the end of a treatment course, which ismore » equivalent to maximizing tumor control probability, subject to the uncertainty inherent in the tumor response. This formulation allows for nonstationary dose distributions as well as nonstationary fractional doses as needed to achieve a series of optimal plans that are conformal to the tumor over time, i.e., spatiotemporally optimal plans. Sixteen phantom cases with various sizes and locations of tumors and organs-at-risk (OAR) were generated using in-house software. Each case was planned with DART and conventional IMRT prescribing 60 Gy in 30 fractions. The observations of the change in the tumor volume over a treatment course were simulated using a two-level cell population model. Monte Carlo simulations of the treatment course for each case were run to account for uncertainty in the tumor response. The same OAR dose constraints were applied for both methods. The frequency of replanning was varied between 1, 2, 5 (weekly), and 29 times (daily). The final average tumor dose and OAR doses have been compared to quantify the potential dosimetric benefits of DART. Results: The average tumor max, min, mean, and D95 doses using DART relative to these using conventional IMRT were 124.0%–125.2%, 102.1%–114.7%, 113.7%–123.4%, and 102.0%–115.9% (range dependent on the frequency of replanning). The average relative maximum doses for the cord and esophagus, mean doses for the heart and lungs, and D05 for the unspecified tissue resulting 84%–102.4%, 99.8%–106.9%, 66.9%–85.6%, 58.2%–78.8%, and 85.2%–94.0%, respectively. Conclusions: It is feasible to apply DART to the treatment of NSCLC using CBCT to observe the midtreatment tumor state. Potential increases in the tumor dose and reductions in the OAR dose, particularly for parallel OARs with mean or dose–volume constraints, could be achieved using DART compared to nonadaptive IMRT.« less

New insights into atmospherically relevant reaction systems using direct analysis in real-time mass spectrometry (DART-MS)

NASA Astrophysics Data System (ADS)

Zhao, Yue; Fairhurst, Michelle C.; Wingen, Lisa M.; Perraud, Véronique; Ezell, Michael J.; Finlayson-Pitts, Barbara J.

2017-04-01

The application of direct analysis in real-time mass spectrometry (DART-MS), which is finding increasing use in atmospheric chemistry, to two different laboratory model systems for airborne particles is investigated: (1) submicron C3-C7 dicarboxylic acid (diacid) particles reacted with gas-phase trimethylamine (TMA) or butylamine (BA) and (2) secondary organic aerosol (SOA) particles from the ozonolysis of α-cedrene. The diacid particles exhibit a clear odd-even pattern in their chemical reactivity toward TMA and BA, with the odd-carbon diacid particles being substantially more reactive than even ones. The ratio of base to diacid in reacted particles, determined using known diacid-base mixtures, was compared to that measured by high-resolution time-of-flight aerosol mass spectrometry (HR-ToF-AMS), which vaporizes the whole particle. Results show that DART-MS probes ˜ 30 nm of the surface layer, consistent with other studies on different systems. For α-cedrene SOA particles, it is shown that varying the temperature of the particle stream as it enters the DART-MS ionization region can distinguish between specific components with the same molecular mass but different vapor pressures. These results demonstrate the utility of DART-MS for (1) examining reactivity of heterogeneous model systems for atmospheric particles and (2) probing components of SOA particles based on volatility.

DART-MS analysis of inorganic explosives using high temperature thermal desorption†‡

PubMed Central

Sisco, Edward; Staymates, Matthew; Gillen, Greg

2018-01-01

An ambient mass spectrometry (MS) platform coupling resistive Joule heating thermal desorption (JHTD) and direct analysis in real time (DART) was implemented for the analysis of inorganic nitrite, nitrate, chlorate, and perchlorate salts. The resistive heating component generated discrete and rapid heating ramps and elevated temperatures, up to approximately 400 °C s−1 and 750 °C, by passing a few amperes of DC current through a nichrome wire. JHTD enhanced the utility and capabilities of traditional DART-MS for the trace detection of previously difficult to detect inorganic compounds. A partial factorial design of experiments (DOE) was implemented for the systematic evaluation of five system parameters. A base set of conditions for JHTD-DART-MS was derived from this evaluation, demonstrating sensitive detection of a range of inorganic oxidizer salts, down to single nanogram levels. DOE also identified JHTD filament current and in-source collision induced dissociation (CID) energy as inducing the greatest effect on system response. Tuning of JHTD current provided a method for controlling the relative degrees of thermal desorption and thermal decomposition. Furthermore, in-source CID provided manipulation of adduct and cluster fragmentation, optimizing the detection of molecular anion species. Finally, the differential thermal desorption nature of the JHTD-DART platform demonstrated efficient desorption and detection of organic and inorganic explosive mixtures, with each desorbing at its respective optimal temperature. PMID:29651308

Attachment process in rocket-triggered lightning strokes

NASA Astrophysics Data System (ADS)

Wang, D.; Rakov, V. A.; Uman, M. A.; Takagi, N.; Watanabe, T.; Crawford, D. E.; Rambo, K. J.; Schnetzer, G. H.; Fisher, R. J.; Kawasaki, Z.-I.

1999-01-01

In order to study the lightning attachment process, we have obtained highly resolved (about 100 ns time resolution and about 3.6 m spatial resolution) optical images, electric field measurements, and channel-base current recordings for two dart leader/return-stroke sequences in two lightning flashes triggered using the rocket-and-wire technique at Camp Blanding, Florida. One of these two sequences exhibited an optically discernible upward-propagating discharge that occurred in response to the approaching downward-moving dart leader and connected to this descending leader. This observation provides the first direct evidence of the occurrence of upward connecting discharges in triggered lightning strokes, these strokes being similar to subsequent strokes in natural lightning. The observed upward connecting discharge had a light intensity one order of magnitude lower than its associated downward dart leader, a length of 7-11 m, and a duration of several hundred nanoseconds. The speed of the upward connecting discharge was estimated to be about 2 × 107 m/s, which is comparable to that of the downward dart leader. In both dart leader/return-stroke sequences studied, the return stroke was inferred to start at the point of junction between the downward dart leader and the upward connecting discharge and to propagate in both upward and downward directions. This latter inference provides indirect evidence of the occurrence of upward connecting discharges in both dart leader/return-stroke sequences even though one of these sequences did not have a discernible optical image of such a discharge. The length of the upward connecting discharges (observed in one case and inferred from the height of the return-stroke starting point in the other case) is greater for the event that is characterized by the larger leader electric field change and the higher return-stroke peak current. For the two dart leader/return-stroke sequences studied, the upward connecting discharge lengths are estimated to be 7-11 m and 4-7 m, with the corresponding return-stroke peak currents being 21 kA and 12 kA, and the corresponding leader electric field changes 30 m from the rocket launcher being 56 kV/m and 43 kV/m. Additionally, we note that the downward dart leader light pulse generally exhibits little variation in its 10-90% risetime and peak value over some tens of meters above the return-stroke starting point, while the following return-stroke light pulse shows an appreciable increase in risetime and a decrease in peak value while traversing the same section of the lightning channel. Our findings regarding (1) the initially bidirectional development of return-stroke process and (2) the relatively strong attenuation of the upward moving return-stroke light (and by inference current) pulse over the first some tens of meters of the channel may have important implications for return-stroke modeling.

Experimental field test of proposed anti-dart-out training programs. Volume 1, Conduct and results

DOT National Transportation Integrated Search

1981-12-01

This report describes the conduct and results of an evaluation of a child pedestrian anti-dart-out training program. Two versions were tested: A film program and a film/simulator program. Before/after accident and street crossing behavior data were c...

The Coriolis Effect: A Model for Student Involvement

ERIC Educational Resources Information Center

Exline, Joseph D.

1977-01-01

Lists materials and procedures for constructing a model that demonstrates certain aspects of the Coriolis effect. Materials include an electric drill motor, voltage control, toy dart gun and darts, wood blocks of varying dimensions. Includes description of an experiment illustrating relationship between speed of rotation and amount of apparent…

Computational Modeling and Simulation of Developmental Toxicity: what can we learn from a virtual embryo? (RIVM, Brussels)

EPA Science Inventory

Developmental and Reproductive Toxicity (DART) testing is important for assessing the potential consequences of drug and chemical exposure on human health and well-being. Complexity of pregnancy and the reproductive cycle makes DART testing challenging and costly for traditional ...

Determination of T-2 and HT-2 toxins from maize by direct analysis in real time - mass spectrometry (DART-MS)

USDA-ARS?s Scientific Manuscript database

Ambient desorption ionization techniques, such as laser desorption with electrospray ionization assistance (ELDI), direct analysis in real time (DART) and desorption electrospray ionization (DESI) have been developed as alternatives to traditional mass spectrometric-based methods. Such techniques al...

A Rather Intelligent Language Teacher.

ERIC Educational Resources Information Center

Cerri, Stefano; Breuker, Joost

1981-01-01

Characteristics of DART (Didactic Augmented Recursive Transition), an ATN-based system for writing intelligent computer assisted instruction (ICAI) programs that is available on the PLATO system are described. DART allows writing programs in an ATN dialect, compiling them in machine code for the PLATO system, and executing them as if the original…

16 CFR 1306.2 - Purpose.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Purpose. 1306.2 Section 1306.2 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.2 Purpose. The purpose of this rule is to prohibit the sale of lawn darts, which have been...

The Single Needle Lockstitch Machine. [Constructing Darts.] Module 3.

ERIC Educational Resources Information Center

South Carolina State Dept. of Education, Columbia. Office of Vocational Education.

This module on constructing darts, one in a series on the single needle lockstitch sewing machine for student self-study, contains two sections. Each section includes the following parts: an introduction, directions, an objective, learning activities, student information, student self-check, check-out activities, and an instructor's final…

16 CFR 1306.2 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Purpose. 1306.2 Section 1306.2 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.2 Purpose. The purpose of this rule is to prohibit the sale of lawn darts, which have been...

16 CFR 1306.2 - Purpose.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Purpose. 1306.2 Section 1306.2 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.2 Purpose. The purpose of this rule is to prohibit the sale of lawn darts, which have been...

16 CFR 1306.2 - Purpose.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Purpose. 1306.2 Section 1306.2 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.2 Purpose. The purpose of this rule is to prohibit the sale of lawn darts, which have been...

Geometrical dart infrared polarization signatures

NASA Astrophysics Data System (ADS)

Lewis, Gareth D.; Jordan, David L.

1996-06-01

The 8 - 12 micrometer polarization signatures of diffuse and specular aluminum geometrical darts were analyzed outdoors using a polarization sensitive thermal imager. Results of the degree and plane of polarization are presented for different thermal imager gain bands and weather conditions during a two week period. The 0 degree, 45 degree, 90 degree and 135 degree polarizer orientations were thermally calibrated and the S1 and S2 Stokes parameters shown as radiometric temperature differences. The effect on the polarization signatures of range is considered for these targets at 100 m and 370 m. A comparison of the degree of polarization to changes in the emission/reflection balance and to variations in the dart's complex refractive index is made.

Time's Arrows? Using a Dartboard Scaffold to Understand Historical Action

ERIC Educational Resources Information Center

Chapman, Arthur

2011-01-01

Arthur Chapman presents a task-specific scaffold--a "dart" board--designed to teach students how to interrogate sources of information so that these become sources of evidence for particular claims about past actions, beliefs and aims. Chapman also uses his "dart" board to foster students' reflection on the degrees of certainty…

16 CFR § 1306.3 - Banned hazardous products.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Banned hazardous products. § 1306.3 Section § 1306.3 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.3 Banned hazardous products. Any lawn dart is a banned...

16 CFR § 1306.2 - Purpose.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Purpose. § 1306.2 Section § 1306.2 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION CONSUMER PRODUCT SAFETY ACT REGULATIONS BAN OF HAZARDOUS LAWN DARTS § 1306.2 Purpose. The purpose of this rule is to prohibit the sale of lawn darts, which...

Mastering the Art of Throwing Darts.

ERIC Educational Resources Information Center

Millward, Robert E.

1998-01-01

Effective leadership programs have a clearly defined belief structure and a teaching team that provides direction for both the students and the program. Leadership can be learned, but it is a process, not a position. Like dart throwing, leadership skills can be taught if individuals are coached, have a chance to practice various skills, get…

Analysis of the aflatoxin AFB1 from corn by direct analysis in real time - mass spectrometry (DART-MS)

USDA-ARS?s Scientific Manuscript database

Direct analysis in real time (DART) ionization coupled to a high resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of aflatoxins extracted from corn. Sample preparation procedure and instrument parameter settings wer...

Impact of Near-Field, Deep-Ocean Tsunami Observations on Forecasting the 7 December 2012 Japanese Tsunami

NASA Astrophysics Data System (ADS)

Bernard, Eddie; Wei, Yong; Tang, Liujuan; Titov, Vasily

2014-12-01

Following the devastating 11 March 2011 tsunami, two deep-ocean assessment and reporting of tsunamis (DART®)(DART® and the DART® logo are registered trademarks of the National Oceanic and Atmospheric Administration, used with permission) stations were deployed in Japanese waters by the Japanese Meteorological Agency. Two weeks after deployment, on 7 December 2012, a M w 7.3 earthquake off Japan's Pacific coastline generated a tsunami. The tsunami was recorded at the two Japanese DARTs as early as 11 min after the earthquake origin time, which set a record as the fastest tsunami detecting time at a DART station. These data, along with those recorded at other DARTs, were used to derive a tsunami source using the National Oceanic and Atmospheric Administration tsunami forecast system. The results of our analysis show that data provided by the two near-field Japanese DARTs can not only improve the forecast speed but also the forecast accuracy at the Japanese tide gauge stations. This study provides important guidelines for early detection and forecasting of local tsunamis.

Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes

PubMed Central

Singh, Vineeta; Gupta, Atul Kumar; Singh, S. P.; Kumar, Anil

2012-01-01

Cinnamomum tamala Nees & Eberm. is an important traditional medicinal plant, mentioned in various ancient literatures such as Ayurveda. Several of its medicinal properties have recently been proved. To characterize diversity in terms of metabolite profiles of Cinnamomum tamala Nees and Eberm genotypes, a newly emerging mass spectral ionization technique direct time in real time (DART) is very helpful. The DART ion source has been used to analyze an extremely wide range of phytochemicals present in leaves of Cinnamomum tamala. Ten genotypes were assessed for the presence of different phytochemicals. Phytochemical analysis showed the presence of mainly terpenes and phenols. These constituents vary in the different genotypes of Cinnamomum tamala. Principal component analysis has also been employed to analyze the DART data of these Cinnamomum genotypes. The result shows that the genotype of Cinnamomum tamala could be differentiated using DART MS data. The active components present in Cinnamomum tamala may be contributing significantly to high amount of antioxidant property of leaves and, in turn, conditional effects for diabetic patients. PMID:22701361

Three unusual strokes in a triggered lightning flash

NASA Technical Reports Server (NTRS)

Idone, V. P.; Orville, R. E.

1984-01-01

Time-resolved photographic records of three strokes of a triggered lightning flash are examined. These strokes exhibit several examples of novel behavior that include (1) the abrupt transformation of dart leaders to stepped leaders and (2) the partial 'reflection' of dart leader luminosity back up the channel. Both phenomena are associated with an apparent discontinuity in channel characteristics between the natural and artificial (wire) sections of the channel. We estimate the ratio of characteristic impedances for these sections to have an upper bound of about a factor of 3. Analysis of one of the dart leader 'reflections' yields a propagation speed of 9.6 x 10 to the 7th m/s whereas the dart leader and return stroke speeds (two-dimensional) over the same channel section are 1.7 x 10 to the 7th and 13 x 10 to the seventh m/s, respectively. Also, one of the return strokes reveals the occurrence of two distinct waves of luminosity that are separated by only 5 microns and that travel up nearly identical channels that differ only in the channel terminus.

Trace analysis of energetic materials via direct analyte-probed nanoextraction coupled to direct analysis in real time mass spectrometry.

PubMed

Clemons, Kristina; Dake, Jeffrey; Sisco, Edward; Verbeck, Guido F

2013-09-10

Direct analysis in real time mass spectrometry (DART-MS) has proven to be a useful forensic tool for the trace analysis of energetic materials. While other techniques for detecting trace amounts of explosives involve extraction, derivatization, solvent exchange, or sample clean-up, DART-MS requires none of these. Typical DART-MS analyses directly from a solid sample or from a swab have been quite successful; however, these methods may not always be an optimal sampling technique in a forensic setting. For example, if the sample were only located in an area which included a latent fingerprint of interest, direct DART-MS analysis or the use of a swab would almost certainly destroy the print. To avoid ruining such potentially invaluable evidence, another method has been developed which will leave the fingerprint virtually untouched. Direct analyte-probed nanoextraction coupled to nanospray ionization-mass spectrometry (DAPNe-NSI-MS) has demonstrated excellent sensitivity and repeatability in forensic analyses of trace amounts of illicit drugs from various types of surfaces. This technique employs a nanomanipulator in conjunction with bright-field microscopy to extract single particles from a surface of interest and has provided a limit of detection of 300 attograms for caffeine. Combining DAPNe with DART-MS provides another level of flexibility in forensic analysis, and has proven to be a sufficient detection method for trinitrotoluene (TNT), RDX, and 1-methylaminoanthraquinone (MAAQ). Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

3D modeling of satellite spectral images, radiation budget and energy budget of urban landscapes

NASA Astrophysics Data System (ADS)

Gastellu-Etchegorry, J. P.

2008-12-01

DART EB is a model that is being developed for simulating the 3D (3 dimensional) energy budget of urban and natural scenes, possibly with topography and atmosphere. It simulates all non radiative energy mechanisms (heat conduction, turbulent momentum and heat fluxes, water reservoir evolution, etc.). It uses DART model (Discrete Anisotropic Radiative Transfer) for simulating radiative mechanisms: 3D radiative budget of 3D scenes and their remote sensing images expressed in terms of reflectance or brightness temperature values, for any atmosphere, wavelength, sun/view direction, altitude and spatial resolution. It uses an innovative multispectral approach (ray tracing, exact kernel, discrete ordinate techniques) over the whole optical domain. This paper presents two major and recent improvements of DART for adapting it to urban canopies. (1) Simulation of the geometry and optical characteristics of urban elements (houses, etc.). (2) Modeling of thermal infrared emission by vegetation and urban elements. The new DART version was used in the context of the CAPITOUL project. For that, districts of the Toulouse urban data base (Autocad format) were translated into DART scenes. This allowed us to simulate visible, near infrared and thermal infrared satellite images of Toulouse districts. Moreover, the 3D radiation budget was used by DARTEB for simulating the time evolution of a number of geophysical quantities of various surface elements (roads, walls, roofs). Results were successfully compared with ground measurements of the CAPITOUL project.

Rapid determination of 1-deoxynojirimycin in Morus alba L. leaves by direct analysis in real time (DART) mass spectrometry.

PubMed

Xu, Bin; Zhang, Dong-Yang; Liu, Ze-Yu; Zhang, Ying; Liu, Li; Li, Long; Liu, Charles C; Wu, Guo-Hua

2015-10-10

A new method based on a Direct Analysis in Real Time (DART) ionization source coupled with triple quadrupole tandem mass spectrometry has been developed for rapid qualitative and quantitative analyses of 1-deoxynojirimycin (DNJ) in mulberry leaves. Two ions produced from DNJ, [M+H](+) (m/z 164) and [M-2H+H](+) (m/z 162), are observed using DART-MS in the positive ion mode. The peak areas of the two selected ions monitoring (SIM) signals of ([M+H](+) (m/z 164) and [M-2H+H](+) (m/z 162)) are integrated to determine the peak area for quantitative analyses. A reasonable linear regression equation is obtained in the range of 1.01 to 40.50 μg/mL, with a linear coefficient (R(2)) of 0.996. The limits of detection (LOD) and quantification (LOQ) of the method are 0.25 and 0.80 μg/mL, respectively. The range of recovery is shown to be 87.73-95.61%. The results derived from the developed DART-MS method are in good agreement with those from the conventional HPLC-FLD method. By contrast, DART-MS in SIM mode is a simple, rapid and high-throughput approach for the determination of the DNJ content in mulberry leaves. The present method is advantageous for the rapid screening of mulberry leaves containing high DNJ contents. Copyright © 2015 Elsevier B.V. All rights reserved.

Forensic applications of direct analysis in real time (DART) coupled to Q-orbitrap tandem mass spectrometry for the in situ analysis of pigments from paint evidence.

PubMed

Chen, Tai-Hung; Wu, Shu-Pao

2017-08-01

The accurate examination of paint fragments obtained from an accident, such as those obtained from vehicles involved in a hit-and-run case, is often critical in forensic investigations. However, organic pigments are typically minor components of automotive coatings, which makes discrimination difficult. In this study, direct analysis in real time coupled to Q-orbitrap tandem mass spectrometry (DART-MS) was employed to detect a wide range of common organic pigments in vehicle paints. Twelve common organic pigments used in vehicle paints, such as red, yellow, orange, and purple, were tested, and a database was constructed for future examinations of vehicle paint. Two hit-and-run vehicle accident cases, which occurred in New Taipei City, were investigated by Fourier transform infrared (FTIR) spectroscopy and DART-MS. First, FTIR spectroscopy was employed to study the paint samples as a preliminary screening step. Most of the observed IR peaks were attributed to binder and extenders present in paints. The IR peaks corresponding to the organic pigments were found to be weak and overlapped with those corresponding to resins. On the other hand, DART-MS successfully characterized the organic pigments. DART-MS was found to be excellent for rapidly determining the presence of organic pigments in paint samples without the need for a complicated pre-treatment process or lengthy analysis time. Copyright © 2017 Elsevier B.V. All rights reserved.

The Asteroid Impact Mission (AIM): Studying the geophysics of small binaries, measuring asteroid deflection and studying impact physics

NASA Astrophysics Data System (ADS)

Kueppers, Michael; Michel, Patrick; AIM Team

2016-10-01

Binary asteroids and their formation mechanisms are of particular interest for understanding the evolution of the small bodies in the solar system. Also, hazards to Earth from impact of near-Earth asteroids and their mitigation have drawn considerable interest over the last decades.Those subjects are both addressed by ESA's Asteroid Impact mission, which is part of the Asteroid Impact & Deflection Assessment (AIDA) currently under study in collaboration between NASA and ESA. NASA's DART mission will impact a projectile into the minor component of the binary near-Earth asteroid (65803) Didymos in 2022. The basic idea is to demonstrate the effect of the impact on the orbital period of the secondary around the primary. ESA's AIM will monitor the Didymos system for several months around the DART impact time.AIM will be launched in aurumn 2020. It is foreseen to arrive at Didymos in April 2022. The mission takes advantage of a close approach of Didymos to Earth. The next opportunity would arise in 2040 only.AIM will stay near Didymos for approximately 6 months. Most of the time it will be placed on the illuminated side of the system, at distances of approximately 35 km and 10 km. AIM is expected to move away from Didymos for some time around the DART impact.The reference payload for AIM includes two visual imagers, a hyperspectral camera, a lidar, a thermal infrared imager, a monostatic high frequency radar, and a bistatic low frequency radar. In addition, AIM will deploy a small lander on the secondary asteroid, and two cubesats that will be used for additional, more risky investigations close to or on the surface of the asteroid.Major contributions from AIM are expected in the study of the geophysics of small asteroids (including for the first time, radar measurements of an interior structure), the formation of binary asteroids, the momentum enhancement factor from the DART impact (through measuring the mass and the change of orbit of the seondary), and impact physics through observing the outcome of an impact with well known impact conditions. In addition, AIM will test new technologies (Cubesats in interplanetary space, Intersatellite links, optical telecommunication in deep space, infrared navigation).

Edinburgh, the Scottish pioneers of anatomy and their lasting influence in South Africa.

PubMed

Correia, J C; Wessels, Q; Vorster, W

2013-11-01

The history of the origin of anatomy education in South Africa is the history of an arduous journey through time. The lasting influence of Edinburgh came in the form of Robert Black Thomson. He was a student and assistant of Sir William Turner who gave rise to the first chair of anatomy and the establishment of a department at the South African College, known today as University of Cape Town. Thomson was later succeeded by Matthew Drennan, a keen anthropologist, who was revered by his students. This Scottish link prevailed over time with the appointment of Edward Philip Stibbe as the chair of anatomy at the South African School of Mines and Technology, which later became the University of the Witwatersrand. Stibbe's successor, Raymond Arthur Dart, a graduate of the University of Sydney, was trained in an anatomy department sculpted on that of Edinburgh by Professor James Thomas Wilson. Wilson's influence at the University of Sydney can be traced back to Edinburgh and William Turner through Thomas Anderson Stuart. Both Dart and Robert Broom, another Scot, were considered as Africa's wild men by the late Professor Tobias. Here, the authors explore the Scottish link and origins of anatomy pedagogy in South Africa.

Global Soil Moisture Estimation through a Coupled CLM4-RTM-DART Land Data Assimilation System

NASA Astrophysics Data System (ADS)

Zhao, L.; Yang, Z. L.; Hoar, T. J.

2016-12-01

Very few frameworks exist that estimate global-scale soil moisture through microwave land data assimilation (DA). Toward this goal, we have developed such a framework by linking the Community Land Model version 4 (CLM4) and a microwave radiative transfer model (RTM) with the Data Assimilation Research Testbed (DART). The deterministic Ensemble Adjustment Kalman Filter (EAKF) within the DART is utilized to estimate global multi-layer soil moisture by assimilating brightness temperature observations from the Advanced Microwave Scanning Radiometer for Earth Observing System (AMSR-E). A 40-member of Community Atmosphere Model version 4 (CAM4) reanalysis is adopted to drive CLM4 simulations. Spatial-specific time-invariant microwave parameters are pre-calibrated to minimize uncertainties in RTM. Besides, various methods are designed in consideration of computational efficiency. A series of experiments are conducted to quantify the DA sensitivity to microwave parameters, choice of assimilated observations, and different CLM4 updating schemes. Evaluation results indicate that the newly established CLM4-RTM-DART framework improves the open-loop CLM4 simulated soil moisture. Pre-calibrated microwave parameters, rather than their default values, can ensure a more robust global-scale performance. In addition, updating near-surface soil moisture is capable of improving soil moisture in deeper layers, while simultaneously updating multi-layer soil moisture fails to obtain intended improvements. We will show in this presentation the architecture of the CLM4-RTM-DART system and the evaluations on AMSR-E DA. Preliminary results on multi-sensor DA that integrates various satellite observations including GRACE, MODIS, and AMSR-E will also be presented. ReferenceZhao, L., Z.-L. Yang, and T. J. Hoar, 2016. Global Soil Moisture Estimation by Assimilating AMSR-E Brightness Temperatures in a Coupled CLM4-RTM-DART System. Journal of Hydrometeorology, DOI: 10.1175/JHM-D-15-0218.1.

Dart model for irradiation-induced swelling of dispersion fuel elements including aluminum-fuel interaction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rest, J.; Hofman, G.L.

1997-06-01

The Dispersion Analysis Research Tool (DART) contains models for fission-gas induced fuel swelling, interaction of fuel with the matrix aluminum, resultant reaction-product swelling, and calculation of the stress gradient within the fuel particle. The effects of an aluminide shell on fuel particle swelling are evaluated. Validation of the model is demonstrated by a comparison of DART calculations of fuel swelling of U{sub 3}SiAl-Al and U{sub 3}Si{sub 2}-Al for various dispersion fuel element designs with the data. DART results are compared with data for fuel swelling Of U{sub 3}SiAl-Al in plate, tube, and rod configurations as a function of fission density.more » Plate and tube calculations were performed at a constant fuel temperature of 373 K and 518 K, respectively. An irradiation temperature of 518 K results in a calculated aluminide layer thickness for the Russian tube that is in the center of the measured range (16 {mu}m). Rod calculations were performed with a temperature gradient across the rod characterized by surface and central temperatures of 373 K and 423 K, respectively. The effective yield stress of irradiated Al matrix material and the aluminide was determined by comparing the results of DART calculations with postirradiation immersion volume measurement of U{sub 3}SiAl plates. The values for the effective yield stress were used in all subsequent simulations. The lower calculated fuel swelling in the rod-type element is due to an assumed biaxial stress state. Fuel swelling in plates results in plate thickness increase only. Likewise, in tubes, only the wall thickness increases. Irradiation experiments have shown that plate-type dispersion fuel elements can develop blisters or pillows at high U-235 burnup when fuel compounds exhibiting breakaway swelling are used at moderate to high fuel volume fractions. DART-calculated interaction layer thickness and fuel swelling follows the trends of the observations. 3 refs., 2 figs.« less

Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia.

PubMed

Wang, Ningning; Wang, Hongyan; Wang, Hui; Zhang, Di; Wu, Ying; Ou, Xiufang; Liu, Shuang; Dong, Zhenying; Liu, Bao

2010-08-26

It is widely recognized that interspecific hybridization may induce "genome shock", and lead to genetic and epigenetic instabilities in the resultant hybrids and/or backcrossed introgressants. A prominent component involved in the genome shock is reactivation of cryptic transposable elements (TEs) in the hybrid genome, which is often associated with alteration in the elements' epigenetic modifications like cytosine DNA methylation. We have previously reported that introgressants derived from hybridization between Oryza sativa (rice) and Zizania latifolia manifested substantial methylation re-patterning and rampant mobilization of two TEs, a copia retrotransposon Tos17 and a MITE mPing. It was not known however whether other types of TEs had also been transpositionally reactivated in these introgressants, their relevance to alteration in cytosine methylation, and their impact on expression of adjacent cellular genes. We document in this study that the Dart TE family was transpositionally reactivated followed by stabilization in all three studied introgressants (RZ1, RZ2 and RZ35) derived from introgressive hybridization between rice (cv. Matsumae) and Z. latifolia, while the TEs remained quiescent in the recipient rice genome. Transposon-display (TD) and sequencing verified the element's mobility and mapped the excisions and re-insertions to the rice chromosomes. Methylation-sensitive Southern blotting showed that the Dart TEs were heavily methylated along their entire length, and moderate alteration in cytosine methylation patterns occurred in the introgressants relative to their rice parental line. Real-time qRT-PCR quantification on the relative transcript abundance of six single-copy genes flanking the newly excised or inserted Dart-related TE copies indicated that whereas marked difference in the expression of all four genes in both tissues (leaf and root) were detected between the introgressants and their rice parental line under both normal and various stress conditions, the difference showed little association with the presence or absence of the newly mobilized Dart-related TEs. Introgressive hybridization has induced transpositional reactivation of the otherwise immobile Dart-related TEs in the parental rice line (cv. Matsumae), which was accompanied with a moderate alteration in the element's cytosine methylation. Significant difference in expression of the Dart-adjacent genes occurred between the introgressants and their rice parental line under both normal and various abiotic stress conditions, but the alteration in gene expression was not coupled with the TEs.

Synchronized observations of cloud-to-ground lightning using VHF broadband interferometer and acoustic arrays

NASA Astrophysics Data System (ADS)

Qiu, Shi; Zhou, Bi-Hua; Shi, Li-Hua

2012-10-01

A single-station-based lightning discharge channel reconstruction system by combining a two-dimensional (2D) VHF broadband interferometer and a three-dimensional (3D) acoustic lighting mapping system has been developed and used for lightning observations. Two cloud-to-ground (CG) flashes with highly branched leaders recorded by the system are analyzed and presented in this paper. VHF radiation could well delineate the development of simultaneous leader branches, while acoustic emissions mainly located on the main channel which was traversed by return stroke (RS) process. Localizations by VHF and acoustic emissions agree well with each other. The mapping results confirm that audible acoustic emission of lightning discharge is mainly associated with high current process like RS. Leaders could generate detectable acoustic signals, with amplitude at least an order weaker than ensuing RS, but they are hard to identify except in closer ranges than the main channel. As a significant phenomenon, this paper provides the first 3D locations associated with sources of tearing sounds, which are inferred to be generated by downward negative leaders when they approach ground. The synchronized observation enable VHF interferometer locate lightning development in spatially quasi 3D, and three stepped leaders, five dart leaders and two dart-stepped leaders are identified, with the 3D velocity (1.3-3.9) × 105 m/s, (1.0-2.9) × 107 m/s and from (1.0-1.3) × 107 m/s to (2.4-2.6) × 106 m/s, respectively. In addition, the application of this approach in improving the accuracy of thunder ranging is discussed.

Design Aids for Real-Time Systems (DARTS)

NASA Technical Reports Server (NTRS)

Szulewski, P. A.

1982-01-01

Design-Aids for Real-Time Systems (DARTS) is a tool that assists in defining embedded computer systems through tree structured graphics, military standard documentation support, and various analyses including automated Software Science parameter counting and metrics calculation. These analyses provide both static and dynamic design quality feedback which can potentially aid in producing efficient, high quality software systems.

Determination of the aflatoxin AFB1 from corn by direct analysis in real time-mass spectrometry (DART-MS)

USDA-ARS?s Scientific Manuscript database

Direct analysis in real time (DART) ionization coupled to a high resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of a common form of aflatoxin, AFB1, extracted from corn. Sample preparation procedure and instrument...

Early Twentieth Century Arrow, Javelin, and Dart Games of the Western Native American.

ERIC Educational Resources Information Center

Pesavento, Wilma J.

The general purpose of this study was to determine whether the traditional native American ball games continued to be positive culture traits of the American Indian in the early twentieth century. The investigation was centered about (1) determining the current arrow, javelin, and dart games of western native Americans, (2) determining the…

Around Marshall

NASA Image and Video Library

1996-06-10

The dart and associated launching system was developed by engineers at MSFC to collect a sample of the aluminum oxide particles during the static fire testing of the Shuttle's solid rocket motor. The dart is launched through the exhaust and recovered post test. The particles are collected on sticky copper tapes affixed to a cylindrical shaft in the dart. A protective sleeve draws over the tape after the sample is collected to prevent contamination. The sample is analyzed under a scarning electron microscope under high magnification and a particle size distribution is determined. This size distribution is input into the analytical model to predict the radiative heating rates from the motor exhaust. Good prediction models are essential to optimizing the development of the thermal protection system for the Shuttle.

Kinesthetic motor imagery training modulates frontal midline theta during imagination of a dart throw.

PubMed

Weber, E; Doppelmayr, M

2016-12-01

Motor imagery (MI) is a frequently used and effective method for motor learning in sports as well as in other domains. Electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) studies indicated that experts within a certain sport exhibit a more pronounced brain activity during MI as compared to novices. Similar to the execution, during MI the motor sequence has to be planned. Thus, the frontal attentional system, in part represented by the frontal midline theta (4-7Hz), is closely related to these processes and presumably plays a major role in MI as well. In this study, a MI dart training and its impact on frontal midline theta activity (fmt) during MI are examined. 53 healthy subjects with no prior dart experience were randomly allocated to a kinesthetic training group (KinVis) or to a control group (Control). Both groups performed 15 training sessions. While in the KinVis group dart throwing was accompanied by MI, the Control group trained without MI. Dart performance and fmt activity during MI within the first and the 15th session were compared. As expected, the performance increase was more pronounced in the KinVis group. Furthermore, frontal theta amplitude was significantly increased in the KinVis group during MI in the 15th training session as compared to the baseline. These results confirm the effectivity of MI. The enhanced fmt activity in the KinVis group can be interpreted as a better allocation of the requested resources in the frontal attentional network after MI. Copyright © 2016 Elsevier B.V. All rights reserved.

Identification of Drugs in Parenteral Pharmaceutical Preparations from a Quality Assurance and a Diversion Program by Direct Analysis in Real-Time AccuTOFTM-Mass Spectrometry (DART-MS).

PubMed

Poklis, Justin L; Mohs, Amanda J; Wolf, Carl E; Poklis, Alphonse; Peace, Michelle R

2016-10-01

In healthcare settings drug diversion and impairment of physicians are major concerns requiring a rapid and efficient method for surveillance and detection. A Direct Analysis in Real Time ion source coupled to a JEOL AccuTOF TM time-of-flight mass spectrometer (DART-MS) method was developed to screen parenteral pharmaceutical formulations for potential drug diversion. Parenteral pharmaceutical formulations are also known as injectable formulations and are used with intravenous, subcutaneous, intramuscular and intra-articular administration. A library was created using the mass spectra data collected by a DART-MS operated in switching mode at 20, 60 and 90 V settings. This library contained 17 commonly encountered drugs in parenteral pharmaceutical formulations that included the surgical analgesic: fentanyl, hydromorphone and morphine; anesthetic: baclofen, bupivacaine, ketamine, midazolam, ropivacaine and succinylcholine; and a mixture of other drug classes: caffeine, clonidine, dexamethasone, ephedrine, heparin, methadone, oxytocin and phenylephrine. Randomly selected 200 de-identified parenteral pharmaceutical formulations containing one or more drugs were submitted for analysis to the FIRM Toxicology Laboratory at Virginia Commonwealth University Health and were screened using the DART-MS. The drug contents of the de-identified formulations were previously confirmed by a published high performance liquid chromatography (HPLC) method. The drugs in the formulations were rapidly and successfully identified using the generated library. The DART-MS and HPLC results were in complete agreement for all 200 parenteral pharmaceutical formulations. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Shared Mechanisms in the Estimation of Self-Generated Actions and the Prediction of Other's Actions by Humans.

PubMed

Ikegami, Tsuyoshi; Ganesh, Gowrishankar

2017-01-01

The question of how humans predict outcomes of observed motor actions by others is a fundamental problem in cognitive and social neuroscience. Previous theoretical studies have suggested that the brain uses parts of the forward model (used to estimate sensory outcomes of self-generated actions) to predict outcomes of observed actions. However, this hypothesis has remained controversial due to the lack of direct experimental evidence. To address this issue, we analyzed the behavior of darts experts in an understanding learning paradigm and utilized computational modeling to examine how outcome prediction of observed actions affected the participants' ability to estimate their own actions. We recruited darts experts because sports experts are known to have an accurate outcome estimation of their own actions as well as prediction of actions observed in others. We first show that learning to predict the outcomes of observed dart throws deteriorates an expert's abilities to both produce his own darts actions and estimate the outcome of his own throws (or self-estimation). Next, we introduce a state-space model to explain the trial-by-trial changes in the darts performance and self-estimation through our experiment. The model-based analysis reveals that the change in an expert's self-estimation is explained only by considering a change in the individual's forward model, showing that an improvement in an expert's ability to predict outcomes of observed actions affects the individual's forward model. These results suggest that parts of the same forward model are utilized in humans to both estimate outcomes of self-generated actions and predict outcomes of observed actions.

75 FR 53271 - Marine Mammals; File No. 15271

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-31

... suction-cup tagged, 10 would be dart-tagged, and 10 would be tagged with small implantable tags; 30 fin whales would be approached for photo-ID and biopsy, of which 10 would be suction-cup tagged, 5 would be... approached for photo-ID and biopsy, of which 30 would be suction-cup tagged, 10 would be dart-tagged, and 10...

Reflecting on the Postgraduate Experience: Teaching Research Methods and Statistics: Review of the DART-P Sponsored Workshop at PsyPAG 2013

ERIC Educational Resources Information Center

Jackson, Emma J.; Davies, Emma. L.

2014-01-01

Following the success of last year's teaching and career development workshop, this year's DART-P sponsored workshop at the Psychology Postgraduate Affairs Group (PsyPAG) Annual Conference held at Lancaster University focused on postgraduate's experiences of teaching research methods. This article provides a review of the invited speakers…

Ability Beliefs, Task Value, and Performance as a Function of Race in a Dart-Throwing Task

ERIC Educational Resources Information Center

Gao, Zan; Kosma, Maria; Harrison, Louis, Jr.

2009-01-01

This study examines differences in self-efficacy, expectancy-related beliefs, task value, and performance in a dart-throwing task as a function of race among diverse college students using the expectancy-value model and self-efficacy theory. It also examines the predictive contributions of these beliefs on task performance within each racial…

Determination of the aflatoxin M1 (AFM1) from milk by direct analysis in real time - mass spectrometry (DART-MS)

USDA-ARS?s Scientific Manuscript database

Certain fungi that grow on crops can produce aflatoxins, which are highly carcinogenic. One of these, aflatoxin B1 can be metabolized by mammals to aflatoxin M1, a form that retains potent carcinogenicity and which can be excreted into milk. Direct analysis in real time (DART) ionization coupled to ...

75 FR 54664 - Self-Regulatory Organizations; BATS Exchange, Inc.; Notice of Filing and Immediate Effectiveness...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-08

... Rule Change To Amend the Name of a BATS Exchange Routing Strategy September 1, 2010. Pursuant to... BATS Rule 11.13(a)(3)(E) to rename the routing strategy identified as ``DART'' to ``DRT''. The text of... 11.13(a)(3)(E) from ``DART'' to ``DRT,'' consistent with the Exchange's re-branding of this routing...

Javelin, Arrow, Dart and Pin Games of Native American Women of the Plains.

ERIC Educational Resources Information Center

Pesavento, Wilma J.; Pesavento, Lisa C.

This study was designed to determine (1) the arrow, dart, javelin, and pin games of Native American girls and women of the Great Plains, (2) the geographical spread of the games within the culture area, and (3) the characteristics of the various games. Data for this investigation were researched from "Annual Reports of the Bureau of American…

Phylogenetic Relationships between Four Salix L. Species Based on DArT Markers

PubMed Central

Przyborowski, Jerzy A.; Sulima, Paweł; Kuszewska, Anna; Załuski, Dariusz; Kilian, Andrzej

2013-01-01

The objectives of this study were to evaluate the usefulness of DArT markers in genotypic identification of willow species and describe genetic relationships between four willow species: Salix viminalis, S. purpurea, S. alba and S. triandra. The experimental plant material comprised 53 willow genotypes of these four species, which are popularly grown in Poland. DArT markers seem to identify Salix species with a high degree of accuracy. As a result, the examined species were divided into four distinct groups which corresponded to the four analyzed species. In our study, we observed that S. triandra was very different genetically from the other species, including S. alba which is generally classified into the same subgenus of Salix. The above corroborates the findings of other authors who relied on molecular methods to reveal that the classification of S. triandra to the subgenus Salix was erroneous. The Principal Coordinate Analysis (PCoA) and the neighbor-joining dendrogram also confirmed the clear division of the studied willow genotypes into four clusters corresponding to individual species. This confirmed the usefulness of DArT markers in taxonomic analyses and identification of willow species. PMID:24336112

Phylogenetic relationships between four Salix L. species based on DArT markers.

PubMed

Przyborowski, Jerzy A; Sulima, Paweł; Kuszewska, Anna; Załuski, Dariusz; Kilian, Andrzej

2013-12-11

The objectives of this study were to evaluate the usefulness of DArT markers in genotypic identification of willow species and describe genetic relationships between four willow species: Salix viminalis, S. purpurea, S. alba and S. triandra. The experimental plant material comprised 53 willow genotypes of these four species, which are popularly grown in Poland. DArT markers seem to identify Salix species with a high degree of accuracy. As a result, the examined species were divided into four distinct groups which corresponded to the four analyzed species. In our study, we observed that S. triandra was very different genetically from the other species, including S. alba which is generally classified into the same subgenus of Salix. The above corroborates the findings of other authors who relied on molecular methods to reveal that the classification of S. triandra to the subgenus Salix was erroneous. The Principal Coordinate Analysis (PCoA) and the neighbor-joining dendrogram also confirmed the clear division of the studied willow genotypes into four clusters corresponding to individual species. This confirmed the usefulness of DArT markers in taxonomic analyses and identification of willow species.

Detection of nitro-organic and peroxide explosives in latent fingermarks by DART- and SALDI-TOF-mass spectrometry.

PubMed

Rowell, Frederick; Seviour, John; Lim, Angelina Yimei; Elumbaring-Salazar, Cheryl Grace; Loke, Jason; Ma, Jan

2012-09-10

The ability of two mass spectrometric methods, surface-assisted laser desorption/ionization-time of flight-mass spectrometry (SALDI-TOF-MS) and direct analysis in real time (DART-MS), to detect the presence of seven common explosives (six nitro-organic- and one peroxide-type) in spiked latent fingermarks has been examined. It was found that each explosive could be detected with nanogram sensitivity for marks resulting from direct finger contact with a glass probe by DART-MS or onto stainless steel target plates using SALDI-TOF-MS for marks pre-dusted with one type of commercial black magnetic powder. These explosives also could be detected in latent marks lifted from six common surfaces (paper, plastic bag, metal drinks can, wood laminate, adhesive tape and white ceramic tile) whereas no explosive could be detected in equivalent pre-dusted marks on the surface of a commercial lifting tape by the DART-MS method due to high background interference from the tape material. The presence of TNT and Tetryl could be detected in pre-dusted latent fingermarks on a commercial lifting tape for up to 29 days sealed and stored under ambient conditions. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Authentication of animal fats using direct analysis in real time (DART) ionization-mass spectrometry and chemometric tools.

PubMed

Vaclavik, Lukas; Hrbek, Vojtech; Cajka, Tomas; Rohlik, Bo-Anne; Pipek, Petr; Hajslova, Jana

2011-06-08

A combination of direct analysis in real time (DART) ionization coupled to time-of-flight mass spectrometry (TOFMS) and chemometrics was used for animal fat (lard and beef tallow) authentication. This novel instrumentation was employed for rapid profiling of triacylglycerols (TAGs) and polar compounds present in fat samples and their mixtures. Additionally, fat isolated from pork, beef, and pork/beef admixtures was analyzed. Mass spectral records were processed by principal component analysis (PCA) and stepwise linear discriminant analysis (LDA). DART-TOFMS profiles of TAGs were found to be more suitable for the purpose of discrimination among the examined fat types as compared to profiles of polar compounds. The LDA model developed using TAG data enabled not only reliable classification of samples representing neat fats but also detection of admixed lard and tallow at adulteration levels of 5 and 10% (w/w), respectively. The presented approach was also successfully applied to minced meat prepared from pork and beef with comparable fat content. Using the DART-TOFMS TAG profiles of fat isolated from meat mixtures, detection of 10% pork added to beef and vice versa was possible.

Three homicides with darts tainted with succinylcholine: autopsy and toxicology.

PubMed

Xing, Jingjun; Li, Wenhe; Tong, Fang; Liang, Yue; He, Guanglong; Zhou, Yiwu

2016-11-01

In emergency departments, intoxication with the muscle relaxant succinylcholine (SUX) often leads to a potentially lethal respiratory paralysis or other deleterious side effects. However, homicide cases with SUX poisoning are very rare because the toxic or lethal concentration ranges of SUX have not yet been determined. We described three uncommon homicide cases due to acute poisoning by darts contaminated with SUX. All the victims died quickly (less than 30 min) after being shot by an especially designed dart gun. Succinylmonocholine (SMC), a metabolite of SUX, was used as a marker to detect the latter. HPLC-MS/MS analysis demonstrated the presence of SUX in the droplet residues of the darts and SMC in the blood and urine in all cases. SMC concentrations of 0.45, 14.0, and 17.9 ng/ml were detected in the victims' blood and 259.0 ng/ml in the urine from the third case. The main pathological changes consisted of hemorrhage of the injured soft tissues, visceral congestion, severe pulmonary edema, and multifocal petechial hemorrhage of the heart and lungs. Taken together, the findings supported a diagnosis of fatal SUX poisoning. Futhermore, our study provided a reference for the lethal concentrations of SUX poisoning.

Tsunami waves generated by dynamically triggered aftershocks of the 2010 Haiti earthquake

NASA Astrophysics Data System (ADS)

Ten Brink, U. S.; Wei, Y.; Fan, W.; Miller, N. C.; Granja, J. L.

2017-12-01

Dynamically-triggered aftershocks, thought to be set off by the passage of surface waves, are currently not considered in tsunami warnings, yet may produce enough seafloor deformation to generate tsunamis on their own, as judged from new findings about the January 12, 2010 Haiti earthquake tsunami in the Caribbean Sea. This tsunami followed the Mw7.0 Haiti mainshock, which resulted from a complex rupture along the north shore of Tiburon Peninsula, not beneath the Caribbean Sea. The mainshock, moreover, had a mixed strike-slip and thrust focal mechanism. There were no recorded aftershocks in the Caribbean Sea, only small coastal landslides and rock falls on the south shore of Tiburon Peninsula. Nevertheless, a tsunami was recorded on deep-sea DART buoy 42407 south of the Dominican Republic and on the Santo Domingo tide gauge, and run-ups of ≤3 m were observed along a 90-km-long stretch of the SE Haiti coast. Three dynamically-triggered aftershocks south of Haiti have been recently identified within the coda of the mainshock (<200 s) by analyzing P wave arrivals recorded by dense seismic arrays, parsing the arrivals into 20-s-long stacks, and back-projecting the arrivals to the vicinity of the main shock (50-300 km). Two of the aftershocks, coming 20-40 s and 40-60 s after the mainshock, plot along NW-SE-trending submarine ridges in the Caribbean Sea south of Haiti. The third event, 120-140 s was located along the steep eastern slope of Bahoruco Peninsula, which is delineated by a normal fault. Forward tsunami models show that the arrival times of the DART buoy and tide gauge times are best fit by the earliest of the three aftershocks, with a Caribbean source 60 km SW of the mainshock rupture zone. Preliminary inversion of the DART buoy time series for fault locations and orientations confirms the location of the first source, but requires an additional unidentified source closer to shore 40 km SW of the mainshock rupture zone. This overall agreement between earthquake and tsunami analyses suggests that land-based earthquake ruptures and/or non-thrust main shocks can generate tsunamis by means of dynamically-triggered aftershocks. It also provides an independent verification to the back-projection seismic method, and it indicates that the active NE-SW shortening of Hispaniola extends southward into the Caribbean Sea.

Profiling of Piper betle Linn. cultivars by direct analysis in real time mass spectrometric technique.

PubMed

Bajpai, Vikas; Sharma, Deepty; Kumar, Brijesh; Madhusudanan, K P

2010-12-01

Piper betle Linn. is a traditional plant associated with the Asian and southeast Asian cultures. Its use is also recorded in folk medicines in these regions. Several of its medicinal properties have recently been proven. Phytochemical analysis showed the presence of mainly terpenes and phenols in betel leaves. These constituents vary in the different cultivars of Piper betle. In this paper we have attempted to profile eight locally available betel cultivars using the recently developed mass spectral ionization technique of direct analysis in real time (DART). Principal component analysis has also been employed to analyze the DART MS data of these betel cultivars. The results show that the cultivars of Piper betle could be differentiated using DART MS data. Copyright © 2010 John Wiley & Sons, Ltd.

Run control techniques for the Fermilab DART data acquisition system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oleynik, G.; Engelfried, J.; Mengel, L.

1995-10-01

DART is the high speed, Unix based data acquisition system being developed by the Fermilab Computing Division in collaboration with eight High Energy Physics Experiments. This paper describes DART run-control which implements flexible, distributed, extensible and portable paradigms for the control and monitoring of data acquisition systems. We discuss the unique and interesting aspects of the run-control - why we chose the concepts we did, the benefits we have seen from the choices we made, as well as our experiences in deploying and supporting it for experiments during their commissioning and sub-system testing phases. We emphasize the software and techniquesmore » we believe are extensible to future use, and potential future modifications and extensions for those we feel are not.« less

Real-time dynamics simulation of the Cassini spacecraft using DARTS. Part 1: Functional capabilities and the spatial algebra algorithm

NASA Technical Reports Server (NTRS)

Jain, A.; Man, G. K.

1993-01-01

This paper describes the Dynamics Algorithms for Real-Time Simulation (DARTS) real-time hardware-in-the-loop dynamics simulator for the National Aeronautics and Space Administration's Cassini spacecraft. The spacecraft model consists of a central flexible body with a number of articulated rigid-body appendages. The demanding performance requirements from the spacecraft control system require the use of a high fidelity simulator for control system design and testing. The DARTS algorithm provides a new algorithmic and hardware approach to the solution of this hardware-in-the-loop simulation problem. It is based upon the efficient spatial algebra dynamics for flexible multibody systems. A parallel and vectorized version of this algorithm is implemented on a low-cost, multiprocessor computer to meet the simulation timing requirements.

Spatial localisation of curcumin and rapid screening of the chemical compositions of turmeric rhizomes (Curcuma longa Linn.) using Direct Analysis in Real Time-Mass Spectrometry (DART-MS).

PubMed

Rahman, A F M Motiur; Angawi, Rihab F; Kadi, Adnan A

2015-04-15

Curcumin is a potent antioxidant agent having versatile biological activities is present in turmeric rhizomes (Curcuma longa Linn.). Powder of turmeric rhizomes is consumes as curry spicy worldwide, especially in Asia. In this study, we demonstrate that, bioactive curcumin and its analog demethoxycurcumin are chiefly concentrated in the pith rather than the other parts of the turmeric rhizomes and it was discovered using modern atmospheric ionisation source 'Direct Analysis in Real Time' (DART) connected with an Ion Trap Mass Spectrometry. In addition, all the major components present in turmeric rhizomes were detected in positive and/or in negative ion mode using DART. Copyright © 2014 Elsevier Ltd. All rights reserved.

Mapping and validation of major quantitative trait loci for kernel length in wild barley (Hordeum vulgare ssp. spontaneum).

PubMed

Zhou, Hong; Liu, Shihang; Liu, Yujiao; Liu, Yaxi; You, Jing; Deng, Mei; Ma, Jian; Chen, Guangdeng; Wei, Yuming; Liu, Chunji; Zheng, Youliang

2016-09-13

Kernel length is an important target trait in barley (Hordeum vulgare L.) breeding programs. However, the number of known quantitative trait loci (QTLs) controlling kernel length is limited. In the present study, we aimed to identify major QTLs for kernel length, as well as putative candidate genes that might influence kernel length in wild barley. A recombinant inbred line (RIL) population derived from the barley cultivar Baudin (H. vulgare ssp. vulgare) and the long-kernel wild barley genotype Awcs276 (H.vulgare ssp. spontaneum) was evaluated at one location over three years. A high-density genetic linkage map was constructed using 1,832 genome-wide diversity array technology (DArT) markers, spanning a total of 927.07 cM with an average interval of approximately 0.49 cM. Two major QTLs for kernel length, LEN-3H and LEN-4H, were detected across environments and further validated in a second RIL population derived from Fleet (H. vulgare ssp. vulgare) and Awcs276. In addition, a systematic search of public databases identified four candidate genes and four categories of proteins related to LEN-3H and LEN-4H. This study establishes a fundamental research platform for genomic studies and marker-assisted selection, since LEN-3H and LEN-4H could be used for accelerating progress in barley breeding programs that aim to improve kernel length.

Injection-Site Reactions in Wild Horses (Equus caballus) Receiving an Immunocontraceptive Vaccine

USGS Publications Warehouse

Roelle, James E.; Ransom, Jason I.

2009-01-01

The U.S. Geological Survey and the Bureau of Land Management are conducting research on the efficacy of the immunocontraceptive agent porcine zona pellucida (PZP) in reducing fertility of wild horses (Equus caballus). As an antigen, PZP stimulates antibody production when injected into many mammalian species. These antibodies bind to the external surface of the ovum, preventing fertilization. By itself, PZP is only weakly immunogenic and is therefore delivered with an adjuvant, most commonly one of the Freund adjuvants, designed to further stimulate antibody production. Freund's complete adjuvant (FCA) in particular is known to be very effective, but may also be associated with undesirable side effects such as formation of abscesses at injection sites. Such reactions may be exacerbated when accompanied by the additional trauma of a remotely delivered dart. Because horses in our three study herds were individually identifiable by color markings and harem association, we were able to monitor mares for injection-site reactions (abscesses, nodules, swelling, and stiffness) following inoculation with PZP. In 100 injections delivered by hand we observed a single nodule, two instances of swelling, and no other reactions. In two herds that received remotely delivered (dart) injections, the frequency of reactions was about 1 and 6 percent for abscesses, 25 percent for nodules (both herds), 11 and 33 percent for swelling, and 1 and 12 percent for stiffness. Abscesses were too infrequent to allow meaningful analysis of the relation to covariates, but for the other types of reactions we used logistic regression to examine the relation of occurrence to the delivery method (rifle or CO2-powered blowgun), adjuvant (FCA, Freund's modified adjuvant, and Freund's incomplete adjuvant), dart trauma (normal or abnormal), and age of mare. Abnormal dart trauma included cases where the dart hit bone or the needle broke off. We found strong evidence (odds ratio = 5.023, P = 0.001) for a higher probability of occurrence of swelling when darts were delivered by blowgun. We found some evidence (odds ratio = 8.729, P = 0.07) that abnormal dart trauma led to a higher frequency of nodule formation. Nodules were the most common reactions observed and often persisted for a year or more, but in our observations they did not appear to change any animal's range of movement or locomotor patterns and in most cases did not appear to differ in magnitude from naturally occurring injuries or scars. We were unable to perform histological examinations of these nodules, but they may be similar to granulomas reported by other investigators following administration of Freund's adjuvant.

Anomalous light output from lightning dart leaders

NASA Technical Reports Server (NTRS)

Guo, C.; Krider, E. P.

1985-01-01

About 5 percent of the multiple-stroke cloud-to-ground lightning discharges recorded at the NASA Kennedy Space Center during the summer of 1981 contained dart leaders that produced an unusually large light output. An analysis of these cases indicates that the average peak light output per unit length in the leader may be comparable to or even exceed that of the return stroke that follows.

Constraints on the perturbed mutual motion in Didymos due to impact-induced deformation of its primary after the DART impact

NASA Astrophysics Data System (ADS)

Hirabayashi, M.; Schwartz, S. R.; Yu, Y.; Davis, A. B.; Chesley, S. R.; Fahnestock, E.; Michel, P.; Richardson, D. C.; Naidu, S.; Scheeres, D. J.; Cheng, A. F.; Rivkin, A.; Benner, L.

2017-12-01

(65803) Didymos is a binary near-Earth asteroid that consists of a top-shaped primary body rotating at a spin period of 2.26 hr and a secondary body orbiting around it at an orbital period of 11.92 hr. This asteroid is the target of the proposed NASA Double Asteroid Redirection Test (DART), which is part of the Asteroid Impact & Deflection Assessment (AIDA) mission concept. The goal of DART is to impact the secondary with the spacecraft and measure the momentum transfer by observing the perturbation of the orbital period of the system after the impact. Achieving this goal requires careful accounting for physical uncertainties that prevent accurate measurement of the momentum transfer. Here, we examine a scenario that might affect the momentum transfer measurement and a possible solution to avoiding issues due to this scenario. The primary's spin period is close to the spin barrier of rubble-pile asteroids, i.e., 2.3 hr. Also, some particles ejected from the secondary due to the DART impact may reach the primary and induce landslides or internal deformation of the primary, changing the gravity field. We have developed a numerical simulation technique for investigating how the mutual orbit of the system varies due to symmetric shape deformation of the primary along its spin axis after the DART impact. We find that if the deformation process occurs, the orbital period can change significantly, depending on the magnitude of the shape deformation. The mission currently plans a nearly head-on collision of the DART impactor with the secondary, making the orbital period of the system shorter. Our simulations show that since the deformation process always causes the primary to become more oblate, it shortens the orbital period as well. We also propose precise measurement of the primary's spin state to determine the deformation of the primary. This relies on the fact that any deformation process changes the spin state of the primary consistent with angular momentum conservation. Further investigations on this problem may improve the accuracy of the momentum transfer measurement for the AIDA mission.

User surveys support designing a prosthetic wrist that incorporates the Dart Thrower's Motion.

PubMed

Davidson, Matthew; Bodine, Cathy; Weir, Richard F Ff

2018-03-07

Prosthetic devices are not meeting the needs of people with upper limb amputations. Due to controlsidelimitations, prosthetic wrists cannot yet be fully articulated. This study sought to determine which wrist motions users felt were most important for completing activities of daily living. We specifically invstigated whether adding a combinationof flexion and deviation known as the Dart Thrower's Motion to a prosthetic wrist would help improve functionality. Fifteen participants with a trans-radial amputation, aged 25-64 years, who use a prosthesis completed an online survey and answered interview questions to determine which types of tasks pose particular challenges. Participants were asked what kinds of improvements they would like to see in a new prosthesis. A subset of five participants were interviewed in-depth to provide further information about difficulties they face using their device. The survey showed that participants had difficulty performing activities of daily living that involve a combination of wrist flexion and deviation known as the "Dart Throwers Motion". Interview responses confirmed that users have difficulty performing these tasks, especially those that require tools. Additionally, users said that they were more interested in having flexion and deviation than rotation in a prosthetic wrist. This research indicates that including the Dart Thrower's Motion in future designs of prosthetic wrists would improve these devices and people with upper limb amputations would be excited to see this improvement in their devices. Implications for Rehabilitation • Over one third of people with upper limb amputations do not use a prosthesis because prosthetic devices do not meet their needs.• The number of motions possible in state of the art prosthetic devices is limited by the small number of control sites available.• The Dart Thrower?s Motion is a wrist motion used for many activities of daily living but unavailable in commercial prosthetics leading many prosthetics users to have difficulty with these tasks.• Prosthetic use, and therefore quality of life, could be improved by including the Dart Thrower's Motion in a prosthesis.

Effect of Micro Porous Shape on Mechanical Properties in Polypropylene Syntactic Foams

NASA Astrophysics Data System (ADS)

Mae, Hiroyuki; Omiya, Masaki; Kishimoto, Kikuo

The objective is to characterize the effect of the microstructure of the micro pores inside the matrix on the mechanical properties of the thermoplastic syntactic polypropylene (PP) foams at the intermediate and high strain rates. Tensile tests are conducted at the nominal strain rates from 3 x 10-1 to 102 s-1. In addition, the dart impact tests are conducted at the impact velocities of 0.1, 1 and 10 m/s. Then, the constitutive law with craze evolution is modified by introducing the relative density, the stress concentration coefficient and the volume fraction of cell edge, and then applied to the dart impact test mode for simulating the macroscopic load displacement history of the dart impact process. Moreover, the microstructural finite element analysis is conducted to characterize the local stress states in the microstructure. In the tensile loading, the elastic modulus is not influenced by the shape of the micro pores in the PP matrix while the yield stress and the strain energy up to failure are relatively influenced by the shape of micro pores. The microstructural finite element analysis shows that the magnitudes of the localized stresses at the edges and the ligaments of the elliptical-shape micro pores are larger than those at the spherical micro pores, leading to the early yielding and the small material ductility. In the case of the dart impact loading, the microstructure of pores has strong effect on the absorbed energy. This is because the elliptical-shape micro pores are very sensitive to the shear deformation, which is revealed by the microstructural finite element analysis. The modified constitutive law with the stress concentration coefficient and the volume fraction of the cell edges successfully predicts the load-displacement curve of the dart impact loading in the spherical micro-porous PP foam. It is concluded that the micro porous shape has strong effect on the material ductility especially in the dart impact test, leading to the possibility to control the material ductility by the shape of the micro pores in the polymeric foams.

The NASA Lightning Nitrogen Oxides Model (LNOM): Application to Air Quality Modeling

NASA Technical Reports Server (NTRS)

Koshak, William; Peterson, Harold; Khan, Maudood; Biazar, Arastoo; Wang, Lihua

2011-01-01

Recent improvements to the NASA Marshall Space Flight Center Lightning Nitrogen Oxides Model (LNOM) and its application to the Community Multiscale Air Quality (CMAQ) modeling system are discussed. The LNOM analyzes Lightning Mapping Array (LMA) and National Lightning Detection Network(TradeMark)(NLDN) data to estimate the raw (i.e., unmixed and otherwise environmentally unmodified) vertical profile of lightning NO(x) (= NO + NO2). The latest LNOM estimates of lightning channel length distributions, lightning 1-m segment altitude distributions, and the vertical profile of lightning NO(x) are presented. The primary improvement to the LNOM is the inclusion of non-return stroke lightning NOx production due to: (1) hot core stepped and dart leaders, (2) stepped leader corona sheath, K-changes, continuing currents, and M-components. The impact of including LNOM-estimates of lightning NO(x) for an August 2006 run of CMAQ is discussed.

Solar array technology evaluation program for SEPS (Solar Electrical Propulsion Stage)

NASA Technical Reports Server (NTRS)

1974-01-01

An evaluation of the technology and the development of a preliminary design for a 25 kilowatt solar array system for solar electric propulsion are discussed. The solar array has a power to weight ratio of 65 watts per kilogram. The solar array system is composed of two wings. Each wing consists of a solar array blanket, a blanket launch storage container, an extension/retraction mast assembly, a blanket tensioning system, an array electrical harness, and hardware for supporting the system for launch and in the operating position. The technology evaluation was performed to assess the applicable solar array state-of-the-art and to define supporting research necessary to achieve technology readiness for meeting the solar electric propulsion system solar array design requirements.

Use of hyaluronidase to improve chemical immobilization of free-ranging polar bears (Ursus maritimus).

PubMed

Cattet, Marc R L; Obbard, Martyn E

2010-01-01

We assessed the efficacy and safety of hyaluronidase to improve chemical immobilization of free-ranging polar bears (Ursus maritimus) captured from helicopter by remote drug delivery along the Ontario coast line of northwestern James Bay and southern Hudson Bay during September 2005 and October 2007. We used a single blind study design in which one person prepared and loaded all darts without the shooter knowing whether hyaluronidase (150 IU per dart) or sterile water was added to the immobilizing drug mixture of xylazine and zolazepam-tiletamine (XZT). We found that we often required more than one dart to immobilize bears in the control group (XZT+sterile water; >1 dart for 15 of 28 captures) versus the treatment group (XZT+hyaluronidase; >1 dart for seven of 26 captures). As a consequence, treatment bears were generally immobilized with smaller XZT dosages (7.9 vs. 9.4 mg/kg; P = 0.08) and shorter induction (10 vs. 15 min; P = 0.004) than control bears. We found no differences in vital rates and serum biochemistry results between control and treatment bears. We did find, however, that induction times correlated directly with rectal temperature at

Acute effect of stretching modalities and time-pressure on accuracy and consistency of throwing darts among 12- and 13-year-old schoolboys.

PubMed

Frikha, Mohamed; Chaâri, Nesrine; Derbel, Mohammad S; Elghoul, Yousri; Zinkovsky, Anatoly V; Chamari, Karim

2017-09-01

The present study addressed the lack of data on the effect of different types of stretching on selected measures of throwing accuracy. We hypothesized that the stretching procedures, within pre-exercise warm-up, could affect the accuracy and the consistency in throwing darts performances under different stress conditions. Eighteen right-handed schoolboys (13.1±0.4 years, 166±0.1 cm and 54.5±9 kg; mean±SD) completed the Darts Throwing Accuracy Test in free (FDT) and in time-pressure (TPDT) conditions, either after static (SS), dynamic (DS), ballistic (BS) or no-stretching (NS) protocols, on nonconsecutive days and in a counter-balanced randomized order. After performing 5 minutes of light standardized jogging and one of the three stretching protocols for 10 minutes, each participant completed the FDT and TPDT tests. Mean scores, missed darts and variability of scores, were recorded and analyzed using a two-way ANOVA with repeated measures. Heart rate (HR), ratings of perceived exertion (RPE) and the task difficulty perception (DP), were recorded through each experimental session. There was no effect of the stretching procedures on accuracy in FDT. However, in the TPDT condition, better performances were recorded after NS and SS compared to DS and BS. The accuracy performances decreased in TPDT by 9.6% after NS (P<0.01); 15.3% after DS (P<0.001) and 11.8% after BS (P<0.001); but not after SS (P<0.05). Static stretching helped reducing the adverse effects of time-pressure on darts throwing performance. Consequently, static exercises are recommended before practicing activities requiring both upper limbs speed and accuracy.

Investigating Marine Boundary Layer Parameterizations by Combining Observations with Models via State Estimation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Delle Monahce, Luca; Clifton, Andrew; Hacker, Joshua

In this project we have improved numerical weather prediction analyses and forecasts of low level winds in the marine boundary layer. This has been accomplished with the following tools; The National Center for Atmospheric Research (NCAR) Weather and Research Forecasting model, WRF, both in his single column (SCM) and three-dimensional (3D) versions; The National Oceanic and Atmospheric Administration (NOAA) Wave Watch III (WWIII); SE algorithms from the Data Assimilation Research Testbed (DART, Anderson et al. 2009); and Observations of key quantities of the lower MBL, including temperature and winds at multiple levels above the sea surface. The experiments with themore » WRF SCM / DART system have lead to large improvements with respect to a standard WRF configuration, which is currently commonly used by the wind energy industry. The single column model appears to be a tool particularly suitable for off-shore wind energy applications given its accuracy, the ability to quantify uncertainty, and the minimal computational resource requirements. In situations where the impact of an upwind wind park may be of interest in a downwind location, a 3D approach may be more suitable. We have demonstrated that with the WRF 3D / DART system the accuracy of wind predictions (and other meteorological parameters) can be improved over a 3D computational domain, and not only at specific locations. All the scripting systems developed in this project (i.e., to run WRF SCM / DART, WRF 3D / DART, and the coupling between WRF and WWIII) and the several modifications and upgrades made to the WRF SCM model will be shared with the broader community.« less

Genetic Structure, Linkage Disequilibrium and Signature of Selection in Sorghum: Lessons from Physically Anchored DArT Markers

PubMed Central

Bouchet, Sophie; Pot, David; Deu, Monique; Rami, Jean-François; Billot, Claire; Perrier, Xavier; Rivallan, Ronan; Gardes, Laëtitia; Xia, Ling; Wenzl, Peter; Kilian, Andrzej; Glaszmann, Jean-Christophe

2012-01-01

Population structure, extent of linkage disequilibrium (LD) as well as signatures of selection were investigated in sorghum using a core sample representative of worldwide diversity. A total of 177 accessions were genotyped with 1122 informative physically anchored DArT markers. The properties of DArTs to describe sorghum genetic structure were compared to those of SSRs and of previously published RFLP markers. Model-based (STRUCTURE software) and Neighbor-Joining diversity analyses led to the identification of 6 groups and confirmed previous evolutionary hypotheses. Results were globally consistent between the different marker systems. However, DArTs appeared more robust in terms of data resolution and bayesian group assignment. Whole genome linkage disequilibrium as measured by mean r2 decreased from 0.18 (between 0 to 10 kb) to 0.03 (between 100 kb to 1 Mb), stabilizing at 0.03 after 1 Mb. Effects on LD estimations of sample size and genetic structure were tested using i. random sampling, ii. the Maximum Length SubTree algorithm (MLST), and iii. structure groups. Optimizing population composition by the MLST reduced the biases in small samples and seemed to be an efficient way of selecting samples to make the best use of LD as a genome mapping approach in structured populations. These results also suggested that more than 100,000 markers may be required to perform genome-wide association studies in collections covering worldwide sorghum diversity. Analysis of DArT markers differentiation between the identified genetic groups pointed out outlier loci potentially linked to genes controlling traits of interest, including disease resistance genes for which evidence of selection had already been reported. In addition, evidence of selection near a homologous locus of FAR1 concurred with sorghum phenotypic diversity for sensitivity to photoperiod. PMID:22428056

Chemometric classification of morphologically similar Umbelliferae medicinal herbs by DART-TOF-MS fingerprint.

PubMed

Lee, Sang Min; Kim, Hye-Jin; Jang, Young Pyo

2012-01-01

It needs many years of special training to gain expertise on the organoleptic classification of botanical raw materials and, even for those experts, discrimination among Umbelliferae medicinal herbs remains an intricate challenge due to their morphological similarity. To develop a new chemometric classification method using a direct analysis in real time-time of flight-mass spectrometry (DART-TOF-MS) fingerprinting for Umbelliferae medicinal herbs and to provide a platform for its application to the discrimination of other herbal medicines. Angelica tenuissima, Angelica gigas, Angelica dahurica and Cnidium officinale were chosen for this study and ten samples of each species were purchased from various Korean markets. DART-TOF-MS was employed on powdered raw materials to obtain a chemical fingerprint of each sample and the orthogonal partial-least squares method in discriminant analysis (OPLS-DA) was used for multivariate analysis. All samples of collected species were successfully discriminated from each other according to their characteristic DART-TOF-MS fingerprint. Decursin (or decursinol angelate) and byakangelicol were identified as marker molecules for Angelica gigas and A. dahurica, respectively. Using the OPLS method for discriminant analysis, Angelica tenuissima and Cnidium officinale were clearly separated into two groups. Angelica tenuissima was characterised by the presence of ligustilide and unidentified molecular ions of m/z 239 and 283, while senkyunolide A together with signals with m/z 387 and 389 were the marker compounds for Cnidium officinale. Elaborating with chemoinformatics, DART-TOF-MS fingerprinting with chemoinformatic tools results in a powerful method for the classification of morphologically similar Umbelliferae medicinal herbs and quality control of medicinal herbal products, including the extracts of these crude drugs. Copyright © 2012 John Wiley & Sons, Ltd.

Shared Mechanisms in the Estimation of Self-Generated Actions and the Prediction of Other’s Actions by Humans

PubMed Central

Ganesh, Gowrishankar

2017-01-01

Abstract The question of how humans predict outcomes of observed motor actions by others is a fundamental problem in cognitive and social neuroscience. Previous theoretical studies have suggested that the brain uses parts of the forward model (used to estimate sensory outcomes of self-generated actions) to predict outcomes of observed actions. However, this hypothesis has remained controversial due to the lack of direct experimental evidence. To address this issue, we analyzed the behavior of darts experts in an understanding learning paradigm and utilized computational modeling to examine how outcome prediction of observed actions affected the participants’ ability to estimate their own actions. We recruited darts experts because sports experts are known to have an accurate outcome estimation of their own actions as well as prediction of actions observed in others. We first show that learning to predict the outcomes of observed dart throws deteriorates an expert’s abilities to both produce his own darts actions and estimate the outcome of his own throws (or self-estimation). Next, we introduce a state-space model to explain the trial-by-trial changes in the darts performance and self-estimation through our experiment. The model-based analysis reveals that the change in an expert’s self-estimation is explained only by considering a change in the individual’s forward model, showing that an improvement in an expert’s ability to predict outcomes of observed actions affects the individual’s forward model. These results suggest that parts of the same forward model are utilized in humans to both estimate outcomes of self-generated actions and predict outcomes of observed actions. PMID:29340300

Sharper and faster "nano darts" kill more bacteria: a study of antibacterial activity of individually dispersed pristine single-walled carbon nanotube.

PubMed

Liu, Shaobin; Wei, Li; Hao, Lin; Fang, Ning; Chang, Matthew Wook; Xu, Rong; Yang, Yanhui; Chen, Yuan

2009-12-22

To further our understanding on the antibacterial activity of single-walled carbon nanotubes (SWCNTs), high purity SWCNTs with average diameter of 0.83 nm and (7,5) chirality as dominate (n,m) structure were dispersed in a biocompatible surfactant solution. Ultraviolet-visible-near-infrared radiation absorption spectroscopy was employed to monitor the aggregation of SWCNTs. The results demonstrated that individually dispersed SWCNTs were more toxic than SWCNT aggregates toward bacteria (gram-negative Escherichia coli, Pseudomonas aeruginosa, and gram-positive Staphylococcus aureus, Bacillus subtilis). Individually dispersed SWCNTs can be visualized as numerous moving "nano darts" in the solution, constantly attacking the bacteria; thereby, degrading the bacterial cell integrity and causing the cell death. Controlled experimental results suggested that inhibiting cell growth and oxidative stress were not the major causes responsible for the death of cells. Furthermore, the detrimental effects of Co metal residues (up to 1 mug/mL) on SWCNT samples can be ruled out. Atomic force microscope study conducted in suspension proved that the death rates of bacteria were strongly correlated with their mechanical properties; soft cells were more vulnerable to SWCNT piercing. The antibacterial activity of SWCNTs can be remarkably improved by enhancing the SWCNT physical puncture on bacteria in the following ways: (1) dispersing SWCNTs individually to sharpen the nano darts; (2) increasing SWCNT concentration to raise the population density of nano darts; and (3) elevating the shaking speed of incubation to speed up the nano darts. This study elucidated several factors controlling the antibacterial activity of pristine SWCNTs and it provided an insight in developing strategies that can maximize the SWCNT application potentials while minimizing the health and environment risks.

Genetic structure, linkage disequilibrium and signature of selection in Sorghum: lessons from physically anchored DArT markers.

PubMed

Bouchet, Sophie; Pot, David; Deu, Monique; Rami, Jean-François; Billot, Claire; Perrier, Xavier; Rivallan, Ronan; Gardes, Laëtitia; Xia, Ling; Wenzl, Peter; Kilian, Andrzej; Glaszmann, Jean-Christophe

2012-01-01

Population structure, extent of linkage disequilibrium (LD) as well as signatures of selection were investigated in sorghum using a core sample representative of worldwide diversity. A total of 177 accessions were genotyped with 1122 informative physically anchored DArT markers. The properties of DArTs to describe sorghum genetic structure were compared to those of SSRs and of previously published RFLP markers. Model-based (STRUCTURE software) and Neighbor-Joining diversity analyses led to the identification of 6 groups and confirmed previous evolutionary hypotheses. Results were globally consistent between the different marker systems. However, DArTs appeared more robust in terms of data resolution and bayesian group assignment. Whole genome linkage disequilibrium as measured by mean r(2) decreased from 0.18 (between 0 to 10 kb) to 0.03 (between 100 kb to 1 Mb), stabilizing at 0.03 after 1 Mb. Effects on LD estimations of sample size and genetic structure were tested using i. random sampling, ii. the Maximum Length SubTree algorithm (MLST), and iii. structure groups. Optimizing population composition by the MLST reduced the biases in small samples and seemed to be an efficient way of selecting samples to make the best use of LD as a genome mapping approach in structured populations. These results also suggested that more than 100,000 markers may be required to perform genome-wide association studies in collections covering worldwide sorghum diversity. Analysis of DArT markers differentiation between the identified genetic groups pointed out outlier loci potentially linked to genes controlling traits of interest, including disease resistance genes for which evidence of selection had already been reported. In addition, evidence of selection near a homologous locus of FAR1 concurred with sorghum phenotypic diversity for sensitivity to photoperiod.

Cities, traffic, and CO 2: A multidecadal assessment of trends, drivers, and scaling relationships

DOE PAGES

Gately, Conor K.; Hutyra, Lucy R.; Sue Wing, Ian

2015-04-06

Emissions of CO 2 from road vehicles were 1.57 billion metric tons in 2012, accounting for 28% of US fossil fuel CO 2 emissions, but the spatial distributions of these emissions are highly uncertain. We develop a new emissions inventory, the Database of Road Transportation Emissions (DARTE), which estimates CO 2 emitted by US road transport at a resolution of 1 km annually for 1980-2012. DARTE reveals that urban areas are responsible for 80% of on-road emissions growth since 1980 and for 63% of total 2012 emissions. We observe nonlinearities between CO 2 emissions and population density at broad spatial/temporalmore » scales, with total on-road CO 2 increasing nonlinearly with population density, rapidly up to 1,650 persons per square kilometer and slowly thereafter. Per capita emissions decline as density rises, but at markedly varying rates depending on existing densities. Here, we make use of DARTE's bottom-up construction to highlight the biases associated with the common practice of using population as a linear proxy for disaggregating national- or state-scale emissions. Comparing DARTE with existing downscaled inventories, we find biases of 100% or more in the spatial distribution of urban and rural emissions, largely driven by mismatches between inventory downscaling proxies and the actual spatial patterns of vehicle activity at urban scales. Here, given cities' dual importance as sources of CO 2 and an emerging nexus of climate mitigation initiatives, high-resolution estimates such as DARTE are critical both for accurately quantifying surface carbon fluxes and for verifying the effectiveness of emissions mitigation efforts at urban scales.« less

Direct Analysis in Real Time-Mass Spectrometry & Kohonen Artificial Neural Networks for the Rapid Species Identification of Larvae, Pupae and Adult Life Stages of Carrion Insects.

PubMed

Beyramysoltan, Samira; Giffen, Justine E; Rosati, Jennifer Y; Musah, Rabi Ann

2018-06-20

Species determination of the various life stages of flies (order: Diptera) is challenging, particularly for the immature forms, because analogous life stages of different species are difficult to differentiate morphologically. It is demonstrated here that DART high-resolution mass spectrometry (DART-HRMS) combined with supervised Kohonen Self-Organizing Maps (SOM) enables accomplishment of species-level identification of larvae, pupae and adult life stages of carrion flies. DART-HRMS data for each life stage were acquired from analysis of ethanol suspensions representing Calliphoridae, Phoridae and Sarcophagidae families, without additional sample preparation. After preprocessing, the data were subjected to a combination of minimum Redundancy Maximal Relevance (mRMR) and Sparse Discriminant Analysis (SDA) methods to select the most significant variables for creating accurate SOM models. The resulting data were divided into training and validation sets, and then analyzed by the SOM method to define the proper discrimination models. The 5-fold venetian blind cross-validation misclassification error was below 7% for all life stages, and the validation samples were correctly identified in all cases. The multiclass SOM model also revealed which chemical components were the most significant markers for each species, with several of these being amino acids. The results show that processing of DART-HRMS data using artificial neural networks (ANNs) based on the Kohonen SOM approach enables rapid discrimination and identification of fly species even for the immature life stages. The ANNs can be continuously expanded to include a larger number of species, and can be used to screen DART-HRMS data from unknowns to rapidly determine species identity.

NOAA Operational Tsunameter Support for Research

NASA Astrophysics Data System (ADS)

Bouchard, R.; Stroker, K.

2008-12-01

In March 2008, the National Oceanic and Atmospheric Administration's (NOAA) National Data Buoy Center (NDBC) completed the deployment of the last of the 39-station network of deep-sea tsunameters. As part of NOAA's effort to strengthen tsunami warning capabilities, NDBC expanded the network from 6 to 39 stations and upgraded all stations to the second generation Deep-ocean Assessment and Reporting of Tsunamis technology (DART II). Consisting of a bottom pressure recorder (BPR) and a surface buoy, the tsunameters deliver water-column heights, estimated from pressure measurements at the sea floor, to Tsunami Warning Centers in less than 3 minutes. This network provides coastal communities in the Pacific, Atlantic, Caribbean, and the Gulf of Mexico with faster and more accurate tsunami warnings. In addition, both the coarse resolution real-time data and the high resolution (15-second) recorded data provide invaluable contributions to research, such as the detection of the 2004 Sumatran tsunami in the Northeast Pacific (Gower and González, 2006) and the experimental tsunami forecast system (Bernard et al., 2007). NDBC normally recovers the BPRs every 24 months and sends the recovered high resolution data to NOAA's National Geophysical Data Center (NGDC) for archive and distribution. NGDC edits and processes this raw binary format to obtain research-quality data. NGDC provides access to retrospective BPR data from 1986 to the present. The DART database includes pressure and temperature data from the ocean floor, stored in a relational database, enabling data integration with the global tsunami and significant earthquake databases. All data are accessible via the Web as tables, reports, interactive maps, OGC Web Map Services (WMS), and Web Feature Services (WFS) to researchers around the world. References: Gower, J. and F. González, 2006. U.S. Warning System Detected the Sumatra Tsunami, Eos Trans. AGU, 87(10). Bernard, E. N., C. Meinig, and A. Hilton, 2007. Deep Ocean Tsunami Detection: Third Generation DART, Eos Trans. AGU, 88(52), Fall Meet. Suppl., Abstract S51C-03.

Simulation of Satellite, Airborne and Terrestrial LiDAR with DART (I):Waveform Simulation with Quasi-Monte Carlo Ray Tracing

NASA Technical Reports Server (NTRS)

Gastellu-Etchegorry, Jean-Philippe; Yin, Tiangang; Lauret, Nicolas; Grau, Eloi; Rubio, Jeremy; Cook, Bruce D.; Morton, Douglas C.; Sun, Guoqing

2016-01-01

Light Detection And Ranging (LiDAR) provides unique data on the 3-D structure of atmosphere constituents and the Earth's surface. Simulating LiDAR returns for different laser technologies and Earth scenes is fundamental for evaluating and interpreting signal and noise in LiDAR data. Different types of models are capable of simulating LiDAR waveforms of Earth surfaces. Semi-empirical and geometric models can be imprecise because they rely on simplified simulations of Earth surfaces and light interaction mechanisms. On the other hand, Monte Carlo ray tracing (MCRT) models are potentially accurate but require long computational time. Here, we present a new LiDAR waveform simulation tool that is based on the introduction of a quasi-Monte Carlo ray tracing approach in the Discrete Anisotropic Radiative Transfer (DART) model. Two new approaches, the so-called "box method" and "Ray Carlo method", are implemented to provide robust and accurate simulations of LiDAR waveforms for any landscape, atmosphere and LiDAR sensor configuration (view direction, footprint size, pulse characteristics, etc.). The box method accelerates the selection of the scattering direction of a photon in the presence of scatterers with non-invertible phase function. The Ray Carlo method brings traditional ray-tracking into MCRT simulation, which makes computational time independent of LiDAR field of view (FOV) and reception solid angle. Both methods are fast enough for simulating multi-pulse acquisition. Sensitivity studies with various landscapes and atmosphere constituents are presented, and the simulated LiDAR signals compare favorably with their associated reflectance images and Laser Vegetation Imaging Sensor (LVIS) waveforms. The LiDAR module is fully integrated into DART, enabling more detailed simulations of LiDAR sensitivity to specific scene elements (e.g., atmospheric aerosols, leaf area, branches, or topography) and sensor configuration for airborne or satellite LiDAR sensors.

Transfer of motor and perceptual skills from basketball to darts

PubMed Central

Rienhoff, Rebecca; Hopwood, Melissa J.; Fischer, Lennart; Strauss, Bernd; Baker, Joseph; Schorer, Jörg

2013-01-01

The quiet eye is a perceptual skill associated with expertise and superior performance; however, little is known about the transfer of quiet eye across domains. We attempted to replicate previous skill-based differences in quiet eye and investigated whether transfer of motor and perceptual skills occurs between similar tasks. Throwing accuracy and quiet eye duration for skilled and less-skilled basketball players were examined in basketball free throw shooting and the transfer task of dart throwing. Skilled basketball players showed significantly higher throwing accuracy and longer quiet eye duration in the basketball free throw task compared to their less-skilled counterparts. Further, skilled basketball players showed positive transfer from basketball to dart throwing in accuracy but not in quiet eye duration. Our results raise interesting questions regarding the measurement of transfer between skills. PMID:24062703

Detection of polydimethylsiloxanes transferred from silicone-coated parchment paper to baked goods using direct analysis in real time mass spectrometry.

PubMed

Jakob, Andreas; Crawford, Elizabeth A; Gross, Jürgen H

2016-04-01

The non-stick properties of parchment papers are achieved by polydimethylsiloxane (PDMS) coatings. During baking, PDMS can thus be extracted from the silicone-coated parchment into the baked goods. Positive-ion direct analysis in real time (DART) mass spectrometry (MS) is highly efficient for the analysis of PDMS. A DART-SVP source was coupled to a quadrupole-time-of-flight mass spectrometer to detect PDMS on the contact surface of baked goods after use of silicone-coated parchment papers. DART spectra from the bottom surface of baked cookies and pizzas exhibited signals because of PDMS ions of the general formula [(C2H6SiO)n  + NH4 ](+) in the m/z 800-1900 range. Copyright © 2016 John Wiley & Sons, Ltd.

The Implementation of Advanced Solar Array Technology in Future NASA Missions

NASA Technical Reports Server (NTRS)

Piszczor, Michael F.; Kerslake, Thomas W.; Hoffman, David J.; White, Steve; Douglas, Mark; Spence, Brian; Jones, P. Alan

2003-01-01

Advanced solar array technology is expected to be critical in achieving the mission goals on many future NASA space flight programs. Current PV cell development programs offer significant potential and performance improvements. However, in order to achieve the performance improvements promised by these devices, new solar array structures must be designed and developed to accommodate these new PV cell technologies. This paper will address the use of advanced solar array technology in future NASA space missions and specifically look at how newer solar cell technologies impact solar array designs and overall power system performance.

AVGS, AR and D for Satellites, ISS, the Moon, Mars and Beyond

NASA Technical Reports Server (NTRS)

Hintze, Geoffrey C.; Cornett, Keith G.; Rahmatipour, Michael H.; Heaton, Andrew F.; Newman, Larry E.; Fleischmann, Kevin D.; Hamby, Byron J.

2007-01-01

With the continuous need to rotate crew and re-supply the International Space Station (ISS) and the desire to return humans to the Moon and for the first time, place humans on Mars, NASA must develop a more robust and highly reliable capability to perform Autonomous Rendezvous and Capture (AR&C) because, unlike the Apollo missions, NASA plans to send the entire crew to the Lunar or Martian surface and must be able to dock with the Orion spacecraft upon return. In 1997, NASA developed the Video Guidance Sensor (VGS) which was flown and tested on STS-87 and STS-95. In 2001, NASA designed and built a more enhanced version of the VGS, called the Advanced Video Guidance Sensor (AVGS). The AVGS offered significant technology improvements to the precursor VGS design. This paper will describe the AVGS as it was in the DART mission of 2005 and the Orbital Express mission of 2007. The paper will describe the capabilities and design concepts of the AVGS as it was flown on the DART 2005 Mission and the DARPA Orbital Express Mission slated to fly in 2007. The paper will cover the Flight Software, problems encountered, testing for Orbital Express and where NASA is going in the future.

Advanced Video Guidance Sensor (AVGS) Development Testing

NASA Technical Reports Server (NTRS)

Howard, Richard T.; Johnston, Albert S.; Bryan, Thomas C.; Book, Michael L.

2004-01-01

NASA's Marshall Space Flight Center was the driving force behind the development of the Advanced Video Guidance Sensor, an active sensor system that provides near-range sensor data as part of an automatic rendezvous and docking system. The sensor determines the relative positions and attitudes between the active sensor and the passive target at ranges up to 300 meters. The AVGS uses laser diodes to illuminate retro-reflectors in the target, a solid-state camera to detect the return from the target, and image capture electronics and a digital signal processor to convert the video information into the relative positions and attitudes. The AVGS will fly as part of the Demonstration of Autonomous Rendezvous Technologies (DART) in October, 2004. This development effort has required a great deal of testing of various sorts at every phase of development. Some of the test efforts included optical characterization of performance with the intended target, thermal vacuum testing, performance tests in long range vacuum facilities, EMI/EMC tests, and performance testing in dynamic situations. The sensor has been shown to track a target at ranges of up to 300 meters, both in vacuum and ambient conditions, to survive and operate during the thermal vacuum cycling specific to the DART mission, to handle EM1 well, and to perform well in dynamic situations.

76 FR 63906 - Henry Gordy International, Inc., Provisional Acceptance of a Settlement Agreement and Order

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-14

...'' (``Target Set''). Each Target Set consisted of the following: A toy gun; soft, pliable, plastic toy darts... report of a death involving a Target Set on or about May 1, 2006, after an 8-year-old boy choked on a dart and died on March 9, 2006. 8. In response to the death reported on or about May 1, 2006, Henry...

The effect of administering long-acting oxytetracycline and tilmicosin either by dart gun or by hand on injection site lesions and drug residues in beef cattle.

PubMed Central

Van Donkersgoed, J; VanderKop, M; Salisbury, C; Sears, L; Holowath, J

1999-01-01

Forty yearling cattle were injected intramuscularly with long-acting oxytetracycline and subcutaneously with tilmicosin by dart gun or by hand in a chute 28 days prior to slaughter. The drugs caused injection site lesions and antibiotic residues in the neck and thigh that varied by technique, dose, and site. PMID:12001341

Data flow manager for DART

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berg, D.; Black, D.; Slimmer, D.

1994-04-01

The DART Data Flow Manager (dfm) integrates a buffer manager with a requester/provider model for scheduling work on buffers. Buffer lists, representing built events or other data, are queued by service requesters to service providers. Buffers may be either internal (reside on the local node), or external (located elsewhere, e.g., dual ported memory). Internal buffers are managed locally. Wherever possible, dfm moves only addresses of buffers rather than buffers themselves.

In Search of the Loci for Sex Differences in Throwing: The Effects of Physical Size and Differential Recruitment Rates on High Levels of Dart Performance

ERIC Educational Resources Information Center

Duffy, Linda J.; Ericsson, K. Anders; Baluch, Bahman

2007-01-01

Contemporary accounts of sex differences in perceptual-motor performance differ in their emphasis on nature and nurture. Study 1 examined the effect of extensive training on one of the largest sex differences, namely accuracy in dart throwing, and found that physical differences in height and reach could not explain sex differences in…

The DART Cylindrical, Infrared, 1 Meter Membrane Reflector

NASA Technical Reports Server (NTRS)

Morgan, Rhonda M.; Agnes, Greg S.; Barber, Dan; Dooley, Jennifer; Dragovan, Mark; Hatheway, Al E.; Marcin, Marty

2004-01-01

The Dual Anamorphic Reflector Telescopes (DART) is an architecture for large aperture space telescopes that enables the use of membranes. A membrane can be readily shaped in one direction of curvature using a combination of boundary control and tensioning, yielding a cylindrical reflector. Two cylindrical reflectors (orthogonal and confocal) comprise the 'primary mirror' of the telescope system. The aperture is completely unobstructed and ideal for infrared and high contrast observations.

Data flow manager for DART

DOE Office of Scientific and Technical Information (OSTI.GOV)

Berg, D.; Black, D.; Slimmer, D.

1994-12-31

The DART Data Flow Manager (dfm) integrates a buffer manager with a requester/provider model for scheduling work on buffers. Buffer lists, representing built events or other data, are queued by service requesters to service providers. Buffers may be either internal (reside on the local node), or external (located elsewhere, e.g., dual ported memory). Internal buffers are managed locally. Wherever possible, dfm moves only addresses of buffers rather than buffers themselves.

Asteroid Impact Mission: relevance to asteroid mining

NASA Astrophysics Data System (ADS)

Michel, P.; Kueppers, M.; Carnelli, I.

2017-09-01

The Asteroid Impact Mission (AIM) is the European (ESA) component of the AIDA mission in collaboration with NASA. The objectives of AIDA are: (1) to perform a test of asteroid deflection using a kinetic impactor with the USA (NASA) component DART, and (2) with AIM, to investigate the binary near-Earth asteroid Didymos, in particular its secondary and target of DART, with data of high value for mining purposes.

High Throughput Ambient Mass Spectrometric Approach to Species Identification and Classification from Chemical Fingerprint Signatures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Musah, Rabi A.; Espinoza, Edgard O.; Cody, Robert B.

A high throughput method for species identification and classification through chemometric processing of direct analysis in real time (DART) mass spectrometry-derived fingerprint signatures has been developed. The method entails introduction of samples to the open air space between the DART ion source and the mass spectrometer inlet, with the entire observed mass spectral fingerprint subjected to unsupervised hierarchical clustering processing. Moreover, a range of both polar and non-polar chemotypes are instantaneously detected. The result is identification and species level classification based on the entire DART-MS spectrum. In this paper, we illustrate how the method can be used to: (1) distinguishmore » between endangered woods regulated by the Convention for the International Trade of Endangered Flora and Fauna (CITES) treaty; (2) assess the origin and by extension the properties of biodiesel feedstocks; (3) determine insect species from analysis of puparial casings; (4) distinguish between psychoactive plants products; and (5) differentiate between Eucalyptus species. An advantage of the hierarchical clustering approach to processing of the DART-MS derived fingerprint is that it shows both similarities and differences between species based on their chemotypes. Furthermore, full knowledge of the identities of the constituents contained within the small molecule profile of analyzed samples is not required.« less

High Throughput Ambient Mass Spectrometric Approach to Species Identification and Classification from Chemical Fingerprint Signatures

DOE PAGES

Musah, Rabi A.; Espinoza, Edgard O.; Cody, Robert B.; ...

2015-07-09

A high throughput method for species identification and classification through chemometric processing of direct analysis in real time (DART) mass spectrometry-derived fingerprint signatures has been developed. The method entails introduction of samples to the open air space between the DART ion source and the mass spectrometer inlet, with the entire observed mass spectral fingerprint subjected to unsupervised hierarchical clustering processing. Moreover, a range of both polar and non-polar chemotypes are instantaneously detected. The result is identification and species level classification based on the entire DART-MS spectrum. In this paper, we illustrate how the method can be used to: (1) distinguishmore » between endangered woods regulated by the Convention for the International Trade of Endangered Flora and Fauna (CITES) treaty; (2) assess the origin and by extension the properties of biodiesel feedstocks; (3) determine insect species from analysis of puparial casings; (4) distinguish between psychoactive plants products; and (5) differentiate between Eucalyptus species. An advantage of the hierarchical clustering approach to processing of the DART-MS derived fingerprint is that it shows both similarities and differences between species based on their chemotypes. Furthermore, full knowledge of the identities of the constituents contained within the small molecule profile of analyzed samples is not required.« less

Evaluation of direct analysis in real time for the determination of highly polar pesticides in lettuce and celery using modified Quick Polar Pesticides Extraction method.

PubMed

Lara, Francisco J; Chan, Danny; Dickinson, Michael; Lloyd, Antony S; Adams, Stuart J

2017-05-05

Direct analysis in real time (DART) was evaluated for the determination of a number of highly polar pesticides using the Quick Polar Pesticides Extraction (QuPPe) method. DART was hyphenated to high resolution mass spectrometry (HRMS) in order to get the required selectivity that allows the determination of these compounds in complex samples such as lettuce and celery. Experimental parameters such as desorption temperature, scanning speed, and distances between the DART ion source and MS inlet were optimized. Two different mass analyzers (Orbitrap and QTOF) and two accessories for sample introduction (Dip-it ® tips and QuickStrip™ sample cards) were evaluated. An extra clean-up step using primary-secondary amine (PSA) was included in the QuPPe method to improve sensitivity. The main limitation found was in-source fragmentation, nevertheless QuPPe-DART-HRMS proved to be a fast and reliable tool with quantitative capabilities for at least seven compounds: amitrole, cyromazine, propamocarb, melamine, diethanolamine, triethanolamine and 1,2,4-triazole. The limits of detection ranged from 20 to 60μg/kg. Recoveries for fortified samples ranged from 71 to 115%, with relative standard deviations <18%. Copyright © 2017 Elsevier B.V. All rights reserved.

A High Throughput Ambient Mass Spectrometric Approach to Species Identification and Classification from Chemical Fingerprint Signatures

PubMed Central

Musah, Rabi A.; Espinoza, Edgard O.; Cody, Robert B.; Lesiak, Ashton D.; Christensen, Earl D.; Moore, Hannah E.; Maleknia, Simin; Drijfhout, Falko P.

2015-01-01

A high throughput method for species identification and classification through chemometric processing of direct analysis in real time (DART) mass spectrometry-derived fingerprint signatures has been developed. The method entails introduction of samples to the open air space between the DART ion source and the mass spectrometer inlet, with the entire observed mass spectral fingerprint subjected to unsupervised hierarchical clustering processing. A range of both polar and non-polar chemotypes are instantaneously detected. The result is identification and species level classification based on the entire DART-MS spectrum. Here, we illustrate how the method can be used to: (1) distinguish between endangered woods regulated by the Convention for the International Trade of Endangered Flora and Fauna (CITES) treaty; (2) assess the origin and by extension the properties of biodiesel feedstocks; (3) determine insect species from analysis of puparial casings; (4) distinguish between psychoactive plants products; and (5) differentiate between Eucalyptus species. An advantage of the hierarchical clustering approach to processing of the DART-MS derived fingerprint is that it shows both similarities and differences between species based on their chemotypes. Furthermore, full knowledge of the identities of the constituents contained within the small molecule profile of analyzed samples is not required. PMID:26156000

A critical evaluation of developmental and reproductive toxicology in nonhuman primates.

PubMed

Faqi, Ali S

2012-02-01

The nonhuman primates (NHPs) are used in many areas of biomedical research where their similarities to humans make them exclusively valuable animal models. The use of NHPs in pre-clinical testing is expected to increase due to the increase in the development of biological compounds for therapeutic uses. The regulatory agencies around the world including Food and Drug Administration (FDA) generally requires developmental and reproductive toxicity (DART) testing of all new drugs to be used by women of childbearing age or men of reproductive potential. NHPs are most frequently used for DART testing when commonly used rodents and/or rabbits are not pharmacologically relevant species. Animal studies are unique in that assessment of reproduction and development as DART studies are not performed in controlled clinical trials; therefore, pre-clinical safety assessment forms the basis for risk assessment for marketed drug products. This paper provides a critical evaluation of developmental and reproductive toxicity studies in NHPs. The manuscript will focus on species selection, limitation of International Conference for Harmonization stages (A-F) using NHPs as a test system, study designs, logistical/technical challenges, and strength, and limitations. It will also pinpoint confounding factors inherent to the test system that may complicate the interpretation of the NHP DART data.

Using non-targeted direct analysis in real time-mass spectrometry (DART-MS) to discriminate seeds based on endogenous or exogenous chemicals.

PubMed

Subbaraj, Arvind K; Barrett, Brent A; Wakelin, Steve A; Fraser, Karl

2015-10-01

Forage seeds are a highly traded agricultural commodity, and therefore, quality control and assurance is high priority. In this study, we have used direct analysis in real time-mass spectrometry (DART-MS) as a tool to discriminate forage seeds based on their non-targeted chemical profiles. In the first experiment, two lots of perennial ryegrass (Lolium perenne L.) seed were discriminated based on exogenous residues of N-(3, 4-dichlorophenyl)-N,N-dimethylurea (Diuron(TM)), a herbicide. In a separate experiment, washed and unwashed seeds of the forage legumes white clover (Trifolium repens L.) and alfalfa (Medicago sativa L.) were discriminated based on the presence or absence of oxylipins, a class of endogenous antimicrobial compounds. Unwashed seeds confer toxicity towards symbiotic, nitrogen-fixing rhizobia which are routinely coated on legume seeds before planting, resulting in reduced rhizobial count. This is the first report of automatic introduction of intact seeds in the DART ion source and detecting oxylipins using DART-MS. Apart from providing scope to investigate legume-rhizobia symbiosis further in the context of oxylipins, the results presented here will enable future studies aimed at classification of seeds based on chemicals bound to the seed coat, thereby offering an efficient screening device for industry.

A High Throughput Ambient Mass Spectrometric Approach to Species Identification and Classification from Chemical Fingerprint Signatures

NASA Astrophysics Data System (ADS)

Musah, Rabi A.; Espinoza, Edgard O.; Cody, Robert B.; Lesiak, Ashton D.; Christensen, Earl D.; Moore, Hannah E.; Maleknia, Simin; Drijfhout, Falko P.

2015-07-01

A high throughput method for species identification and classification through chemometric processing of direct analysis in real time (DART) mass spectrometry-derived fingerprint signatures has been developed. The method entails introduction of samples to the open air space between the DART ion source and the mass spectrometer inlet, with the entire observed mass spectral fingerprint subjected to unsupervised hierarchical clustering processing. A range of both polar and non-polar chemotypes are instantaneously detected. The result is identification and species level classification based on the entire DART-MS spectrum. Here, we illustrate how the method can be used to: (1) distinguish between endangered woods regulated by the Convention for the International Trade of Endangered Flora and Fauna (CITES) treaty; (2) assess the origin and by extension the properties of biodiesel feedstocks; (3) determine insect species from analysis of puparial casings; (4) distinguish between psychoactive plants products; and (5) differentiate between Eucalyptus species. An advantage of the hierarchical clustering approach to processing of the DART-MS derived fingerprint is that it shows both similarities and differences between species based on their chemotypes. Furthermore, full knowledge of the identities of the constituents contained within the small molecule profile of analyzed samples is not required.

CNEA/ANL collaboration program to develop an optimized version of DART validation and assessment by means of U{sub 3}Si{sub x} and U{sub 3}O{sub 8-}Al dispersed CNEA miniplate irradiation behavior.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Solis, D.

1998-10-16

The DART code is based upon a thermomechanical model that can predict swelling, recrystallization, fuel-meat interdiffusion and other issues related with MTR dispersed FE behavior under irradiation. As a part of a common effort to develop an optimized version of DART, a comparison between DART predictions and CNEA miniplates irradiation experimental data was made. The irradiation took place during 1981-82 for U3O8 miniplates and 1985-86 for U{sub 3}Si{sub x} at Oak Ridge Research Reactor (ORR). The microphotographs were studied by means of IMAWIN 3.0 Image Analysis Code and different fission gas bubbles distributions were obtained. Also it was possible tomore » find and identify different morphologic zones. In both kinds of fuels, different phases were recognized, like particle peripheral zones with evidence of Al-U reaction, internal recrystallized zones and bubbles. A very good agreement between code prediction and irradiation results was found. The few discrepancies are due to local, fabrication and irradiation uncertainties, as the presence of U{sub 3}Si phase in U{sub 3}Si{sub 2} particles and effective burnup.« less

The treatment of solid tumors by alpha emitters released from 224Ra-loaded sources—internal dosimetry analysis

NASA Astrophysics Data System (ADS)

Arazi, L.; Cooks, T.; Schmidt, M.; Keisari, Y.; Kelson, I.

2010-02-01

Diffusing alpha-emitters radiation therapy (DART) is a proposed new form of brachytherapy, allowing the treatment of solid tumors by alpha particles. DART utilizes implantable sources carrying small activities of radium-224, which continually release into the tumor radon-220, polonium-216 and lead-212 atoms, while radium-224 itself remains fixed to the source. The released atoms disperse inside the tumor by diffusive and convective processes, creating, through their alpha emissions, a high-dose region measuring several mm in diameter about each source. The efficacy of DART has been demonstrated in preclinical studies on mice-borne squamous cell carcinoma and lung tumors and the method is now being developed toward clinical trials. This work studies DART safety with respect to the dose delivered to distant organs as a result of lead-212 leakage from the tumor through the blood, relying on a biokinetic calculation coupled to internal dose assessments. It is found that the dose-limiting organs are the kidneys and red bone marrow. Assuming a typical source spacing of ~5 mm and a typical radium-224 activity density of 0.4-0.8 MBq g-1 of tumor tissue, it is predicted that tumors weighing up to several hundred grams may be treated without reaching the tolerance dose in any organ.

Trace-Level Volatile Quantitation by Direct Analysis in Real Time Mass Spectrometry following Headspace Extraction: Optimization and Validation in Grapes.

PubMed

Jastrzembski, Jillian A; Bee, Madeleine Y; Sacks, Gavin L

2017-10-25

Ambient ionization mass spectrometric (AI-MS) techniques like direct analysis in real time (DART) offer the potential for rapid quantitative analyses of trace volatiles in food matrices, but performance is generally limited by the lack of preconcentration and extraction steps. The sensitivity and selectivity of AI-MS approaches can be improved through solid-phase microextraction (SPME) with appropriate thin-film geometries, for example, solid-phase mesh-enhanced sorption from headspace (SPMESH). This work improves the SPMESH-DART-MS approach for use in food analyses and validates the approach for trace volatile analysis for two compounds in real samples (grape macerates). SPMESH units prepared with different sorbent coatings were evaluated for their ability to extract a range of odor-active volatiles, with poly(dimethylsiloxane)/divinylbenzene giving the most satisfactory results. In combination with high-resolution mass spectrometry (HRMS), detection limits for SPMESH-DART-MS under 4 ng/L in less than 30 s acquisition times could be achieved for some volatiles [3-isobutyl-2-methoxypyrazine (IBMP) and β-damascenone]. A comparison of SPMESH-DART-MS and SPME-GC-MS quantitation of linalool and IBMP demonstrates excellent agreement between the two methods for real grape samples (r 2 ≥ 0.90), although linalool measurements appeared to also include isobaric interference.

Rapid analysis of caffeine in various coffee samples employing direct analysis in real-time ionization-high-resolution mass spectrometry.

PubMed

Danhelova, Hana; Hradecky, Jaromir; Prinosilova, Sarka; Cajka, Tomas; Riddellova, Katerina; Vaclavik, Lukas; Hajslova, Jana

2012-07-01

The development and use of a fast method employing a direct analysis in real time (DART) ion source coupled to high-resolution time-of-flight mass spectrometry (TOFMS) for the quantitative analysis of caffeine in various coffee samples has been demonstrated in this study. A simple sample extraction procedure employing hot water was followed by direct, high-throughput (<1 min per run) examination of the extracts spread on a glass rod under optimized conditions of ambient mass spectrometry, without any prior chromatographic separation. For quantification of caffeine using DART-TOFMS, an external calibration was used. Isotopically labeled caffeine was used to compensate for the variations of the ion intensities of caffeine signal. Recoveries of the DART-TOFMS method were 97% for instant coffee at the spiking levels of 20 and 60 mg/g, respectively, while for roasted ground coffee, the obtained values were 106% and 107% at the spiking levels of 10 and 30 mg/g, respectively. The repeatability of the whole analytical procedure (expressed as relative standard deviation, RSD, %) was <5% for all tested spiking levels and matrices. Since the linearity range of the method was relatively narrow (two orders of magnitude), an optimization of sample dilution prior the DART-TOFMS measurement to avoid saturation of the detector was needed.

Alternative mass reference standards for direct analysis in real time mass spectrometry.

PubMed

Cody, Robert B; Dane, A John

2016-05-30

Mass spectra were acquired with the Direct Analysis in Real Time (DART®) ion source for an amine-terminated polyether used as positive-ion mass reference standards and for several fluorinated materials commonly used as negative-ion reference standards for mass spectrometry. A commercial time-of-flight mass spectrometer equipped with a DART ion source was used for all measurements. Mass reference standards deposited onto the sealed end of a glass melting point tube were suspended in the DART gas stream for analysis. A polyetheramine (Jeffamine® M-600) produced intense peaks corresponding to protonated molecules. Perfluorotributylamine (PFTBA), and perfluorotripentylamine, gave useful reference spectra for different m/z ranges. DART mass spectra of Ultramark 1621® resembled those previously reported for Fast Atom Bombardment (FAB) and Electrospray Ionization (ESI). Fomblin®Y, a fluorinated ether, was the most useful negative-ion reference standard of the materials tested. The material is commercially available, inexpensive, and provides reference peaks covering the m/z range 85 to >3000. Jeffamine-M600 was found to be a convenient alternative to polyethers such as polyethylene glycol (PEG) for DART positive-ion mass calibration. Fomblin Y was suitable for use as a negative-ion reference standard. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

Phased-array-fed antenna configuration study. Volume 1: Technology assessment

NASA Technical Reports Server (NTRS)

Sorbello, R. M.; Zaghloul, A. I.; Lee, B. S.; Siddiqi, S.; Geller, B. D.; Gerson, H. I.; Srinivas, D. N.

1983-01-01

The status of the technologies for phased-array-fed dual reflector systems is reviewed. The different aspects of these technologies, including optical performances, phased array systems, problems encountered in phased array design, beamforming networks, MMIC design and its incorporation into waveguide systems, reflector antenna structures, and reflector deployment mechanisms are addressed.

Access to CAMAC from VxWorks and UNIX in DART

DOE Office of Scientific and Technical Information (OSTI.GOV)

Streets, J.; Meadows, J.; Moore, C.

1995-05-01

As part of the DART Project the authors have developed a package of software for CAMAC access from UNIX and VxWorks platforms, with support for several hardware interfaces. They report on developments for the CES CBD8210 VME to parallel CAMAC, the Hytec VSD2992 VME to serial CAMAC and Jorway 411S SCSI to parallel and serial CAMAC branch drivers, and give a summary of the timings obtained.

Second-Tier Database for Ecosystem Focus, 2002-2003 Annual Report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Van Holmes, Chris; Muongchanh, Christine; Anderson, James J.

2003-11-01

The Second-Tier Database for Ecosystem Focus (Contract 00004124) provides direct and timely public access to Columbia Basin environmental, operational, fishery and riverine data resources for federal, state, public and private entities. The Second-Tier Database known as Data Access in Realtime (DART) integrates public data for effective access, consideration and application. DART also provides analysis tools and performance measures helpful in evaluating the condition of Columbia Basin salmonid stocks.

Infection Risk From Conducted Electrical Weapon Probes: What Do We Know?

PubMed

Kroll, Mark W; Ritter, Mollie B; Guilbault, Richard A; Panescu, Dorin

2016-11-01

Concern has been raised over the infection risk of the TASER electrical weapon since the probes penetrate the skin. The manufacturing process produces unsterilized probes with a 5% rate of Staphylococcus aureus contamination. Voluntary recipients (n = 208) of probe exposures were surveyed and there were no self-observations of infection. With over 3.3 million probe landings, there have been 10 case reports of penetrations of sensitive tissue with no reported infections. The electrical field was modeled and found that the electrical pulses generate a field of over 1200 V/mm on the dart portion. This is sufficient to sterilize the dart via electroporation. Electrical weapon probes appear to have a very low (possibly zero) rate of infection. The factors leading to this low infection rate appear to be a manufacturing process producing a low rate of bacterial contamination and the pulses sterilizing the dart via electroporation. © 2016 American Academy of Forensic Sciences.

Analysis of hairy root culture of Rauvolfia serpentina using direct analysis in real time mass spectrometric technique.

PubMed

Madhusudanan, K P; Banerjee, Suchitra; Khanuja, Suman P S; Chattopadhyay, Sunil K

2008-06-01

The applicability of a new mass spectrometric technique, DART (direct analysis in real time) has been studied in the analysis of the hairy root culture of Rauvolfia serpentina. The intact hairy roots were analyzed by holding them in the gap between the DART source and the mass spectrometer for measurements. Two nitrogen-containing compounds, vomilenine and reserpine, were characterized from the analysis of the hairy roots almost instantaneously. The confirmation of the structures of the identified compounds was made through their accurate molecular formula determinations. This is the first report of the application of DART technique for the characterization of compounds that are expressed in the hairy root cultures of Rauvolfia serpentina. Moreover, this also constitutes the first report of expression of reserpine in the hairy root culture of Rauvolfia serpentina. Copyright (c) 2008 John Wiley & Sons, Ltd.

A compressed sensing based approach on Discrete Algebraic Reconstruction Technique.

PubMed

Demircan-Tureyen, Ezgi; Kamasak, Mustafa E

2015-01-01

Discrete tomography (DT) techniques are capable of computing better results, even using less number of projections than the continuous tomography techniques. Discrete Algebraic Reconstruction Technique (DART) is an iterative reconstruction method proposed to achieve this goal by exploiting a prior knowledge on the gray levels and assuming that the scanned object is composed from a few different densities. In this paper, DART method is combined with an initial total variation minimization (TvMin) phase to ensure a better initial guess and extended with a segmentation procedure in which the threshold values are estimated from a finite set of candidates to minimize both the projection error and the total variation (TV) simultaneously. The accuracy and the robustness of the algorithm is compared with the original DART by the simulation experiments which are done under (1) limited number of projections, (2) limited view problem and (3) noisy projections conditions.

Detailed Modeling of the DART Spacecraft Impact into Didymoon

NASA Astrophysics Data System (ADS)

Weaver, R.; Gisler, G.

2017-12-01

In this presentation we will model the impact of the DART spacecraft into the target Didymoon. Most previous modeling of this impact has used full density aluminum spheres with a mass of 300 kg or more recently 500 kg. Many of the published scaling laws for crater size and diameter as well as ejecta modeling assume this type of impactor. The actual spacecraft for the DART impact is not solid and does not contain a solid dedicated kinetic impactor. The spacecraft is considered the impactor. Since the spacecraft is significantly larger ( 100 x 100 x 200 cm) in size than a full density aluminum sphere (radius 35 cm) the resulting impact dynamics will be quite different. Here we model both types of impact and compare the results of the simulation for crater size, crater depth and ejecta. This allows for a comparison of the momentum enhancement factor, beta. Suggestions for improvement of the spacecraft design will be given.

Recent developments in the field of arrow and dart poisons.

PubMed

Philippe, Geneviève; Angenot, Luc

2005-08-22

Arrow and dart poisons, considered as conventional natural sources for future drug discovery, have already provided numerous biologically active molecules used as drugs in therapeutic applications or in pharmacological research. Plants containing alkaloids or cardiotonic glycosides have generally been the main ingredients responsible for the efficacy of these poisons, although some animals, such as frogs, have also been employed. This paper, without being exhaustive, reports the greater strides made during the past 15 years in the understanding of the chemical nature and biological properties of arrow and dart poison constituents. Examples both of promising biological properties shown by these molecules and of crucial discoveries achieved by their use as pharmacological tools are given. Further studies of these toxic principles are likely to enable scientists to find new valuable lead compounds, useful in many fields of research, like oncology, inflammation and infectious diseases.

Real-time dynamic simulation of the Cassini spacecraft using DARTS. Part 2: Parallel/vectorized real-time implementation

NASA Technical Reports Server (NTRS)

Fijany, A.; Roberts, J. A.; Jain, A.; Man, G. K.

1993-01-01

Part 1 of this paper presented the requirements for the real-time simulation of Cassini spacecraft along with some discussion of the DARTS algorithm. Here, in Part 2 we discuss the development and implementation of parallel/vectorized DARTS algorithm and architecture for real-time simulation. Development of the fast algorithms and architecture for real-time hardware-in-the-loop simulation of spacecraft dynamics is motivated by the fact that it represents a hard real-time problem, in the sense that the correctness of the simulation depends on both the numerical accuracy and the exact timing of the computation. For a given model fidelity, the computation should be computed within a predefined time period. Further reduction in computation time allows increasing the fidelity of the model (i.e., inclusion of more flexible modes) and the integration routine.

Dynamic motion analysis of dart throwers motion visualized through computerized tomography and calculation of the axis of rotation.

PubMed

Edirisinghe, Y; Troupis, J M; Patel, M; Smith, J; Crossett, M

2014-05-01

We used a dynamic three-dimensional (3D) mapping method to model the wrist in dynamic unrestricted dart throwers motion in three men and four women. With the aid of precision landmark identification, a 3D coordinate system was applied to the distal radius and the movement of the carpus was described. Subsequently, with dynamic 3D reconstructions and freedom to position the camera viewpoint anywhere in space, we observed the motion pathways of all carpal bones in dart throwers motion and calculated its axis of rotation. This was calculated to lie in 27° of anteversion from the coronal plane and 44° of varus angulation relative to the transverse plane. This technique is a safe and a feasible carpal imaging method to gain key information for decision making in future hand surgical and rehabilitative practices.

Photovoltaic options for solar electric propulsion

NASA Technical Reports Server (NTRS)

Stella, Paul M.; Flood, Dennis J.

1990-01-01

During the past decade, a number of advances have occurred in solar cell and array technology. These advances have lead to performance improvement for both conventional space arrays and for advanced technology arrays. Performance enhancements have occurred in power density, specific power, and environmental capability. Both state-of-the-art and advanced development cells and array technology are discussed. Present technology will include rigid, rollout, and foldout flexible substrate designs, with silicon and GaAs solar cells. The use of concentrator array systems is also discussed based on both DOD and NASA efforts. The benefits of advanced lightweight array technology, for both near term and far term utilization, and of advanced high efficiency, thin, radiation resistant cells is examined. This includes gallium arsenide on germaniun substrates, indium phosphide, and thin film devices such as copper indium diselenide.

High-power, ultralow-mass solar arrays: FY-77 solar arrays technology readiness assessment report, volume 2

NASA Technical Reports Server (NTRS)

Costogue, E. N.; Young, L. E.; Brandhorst, H. W., Jr.

1978-01-01

Development efforts are reported in detail for: (1) a lightweight solar array system for solar electric propulsion; (2) a high efficiency thin silicon solar cell; (3) conceptual design of 200 W/kg solar arrays; (4) fluorocarbon encapsulation for silicon solar cell array; and (5) technology assessment of concentrator solar arrays.

Evaluation of space station solar array technology

NASA Technical Reports Server (NTRS)

1972-01-01

The research concerning lightweight solar array assemblies since 1970 is reported. A bibliography of abstracts of documents used for reference during this period is included along with an evaluation of available solar array technology. A list of recommended technology programs is presented.

Collection and analysis of wind data for the evaluation of Wildland-Urban Interface Fire Dynamics Simulator

NASA Astrophysics Data System (ADS)

Espina, Chad Edward Obedoza

The Wildland Urban-Interface Fire Dynamics Simulator (WFDS) is a computer code that is currently being developed by the National Institute of Standards and Technology (NIST). WFDS has the capability of simulating wildland fire behavior with prescribed elements such vegetative and structural fuel, topography, and weather conditions. In this initial stage of the research, support for the development of WFDS focuses on the evaluation of a wind flow simulation on a very complex, outdoor terrain. This effort is preceded by the fabrication, installation and testing of wind-sensing equipment. Foremost, wind data gathered from different sites using various instruments are compared and evaluated. The data gathered in the Trails community of Rancho Bernardo is then presented and compared to select WFDS simulations. Systems consisting of a wind vane and anemometer are currently installed in the Trails community of Rancho Bernardo. They were installed by Professor Fletcher J. Miller and me using a lift that is attached to a telescoping crane. These instruments will gather the wind data needed to show the behavioral patterns of winds influenced by the topography and obstructions such as trees and houses. They are currently installed on top of light posts. These light posts were picked based on the path of the fire influenced by the Santa Ana winds that ravaged the community in 2007. The data from these instruments were graphically represented using a Matlab code that was developed specifically for the data sets. The Matlab graphing utility plots wind speed and wind direction along with matching polar plots. Other main features also include the ability to set a time range and compare two sites in one plot. There are other wind instruments currently being tested and being analyzed to ensure correct data is being recorded. These instruments will also expand to a wider range the wind data-gathering capabilities vertically. A Sound Detecting and Ranging (SoDAR) unit gathers wind speed and direction from the sound waves, initially emitted by the SoDAR to the atmosphere, that are reflected by the air flow above the unit. Wind data has been compared to the SoDAR unit with data from instruments installed on a meteorological tower operated by the National Oceanic and Atmospheric Administration (NOAA) located in northern California. Two more SoDARs are currently in Texas where initially they were deployed 400 meters apart of each other at an airfield. Also in the same airfield, the wind instrument of an Unmanned Aerial Vehicle (UAV) SuperBat was tested and compared to the SoDARs. Lastly, a self-contained wind instrument (Wind Dart) on a UAV that was developed by the University of Colorado was tested. The instrument was used while attached to the UAV Spectra. A static test was also done in San Diego State University's low speed wind tunnel. The wind data comparison from the SoDAR and meteorological tower in Lodi, California showed close tracking to each other both in wind speed and direction. The comparison of the wind data gathered by the two SoDARs in Texas also showed close tracking to each other. As for the Wind Dart, the data gathered from the instrument and UAV Spectra are not conclusive enough to validate the abilities of the Wind Dart. The experimental procedure in testing the Wind Dart on a moving platform must be further developed. Before the aerial test of the Wind Dart, it was first tested at San Diego State University's low speed tunnel. The detected wind speed by the Wind Dart closely matches the prescribed wind speed of the wind tunnel. The data between the UAV SuperBat and SoDARs showed close tracking. Data collected by the Rancho Bernardo wind instruments shows cyclical wind patterns in the neighborhood. Initial evaluation of select WFDS simulations show data that mimics data gathered from the field.

DART Support for Hurricane Matthew

NASA Image and Video Library

2016-10-13

Members of the Disaster Assessment and Recovery Team (DART) work on flooring repairs to the Beach House at NASA’s Kennedy Space Center in Florida. The effort is part of the spaceport’s ongoing recovery from Hurricane Matthew, which passed to the east of Kennedy on Oct. 6 and 7, 2016. The center received some isolated roof damage, damaged support buildings, a few downed power lines, and limited water intrusion. Beach erosion also occurred, although the storm surge was less than expected.

Design Through Analysis (DTA) roadmap vision.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Blacker, Teddy Dean; Adams, Charles R.; Hoffman, Edward L.

2004-10-01

The Design through Analysis Realization Team (DART) will provide analysts with a complete toolset that reduces the time to create, generate, analyze, and manage the data generated in a computational analysis. The toolset will be both easy to learn and easy to use. The DART Roadmap Vision provides for progressive improvements that will reduce the Design through Analysis (DTA) cycle time by 90-percent over a three-year period while improving both the quality and accountability of the analyses.

Direct analysis in real time mass spectrometry (DART-MS) of highly non-polar low molecular weight polyisobutylenes.

PubMed

Nagy, Lajos; Nagy, Tibor; Deák, György; Kuki, Ákos; Antal, Borbála; Zsuga, Miklós; Kéki, Sándor

2015-09-01

Low molecular weight polyisobutylenes (PIB) with chlorine, olefin and succinic acid end-groups were studied using direct analysis in real time mass spectrometry (DART-MS). To facilitate the adduct ion formation under DART conditions, NH 4 Cl as an auxiliary reagent was deposited onto the PIB surface. It was found that chlorinated adduct ions of olefin and chlorine telechelic PIBs, i.e. [M + Cl] - up to m/z 1100, and the deprotonated polyisobutylene succinic acid [MH] - were formed as observed in the negative ion mode. In the positive ion mode formation of [M + NH 4 ] + , adduct ions were detected. In the tandem mass (MS/MS) spectra of [M + Cl] - , product ions were absent, suggesting a simple dissociation of the precursor [M + Cl] - into a Cl - ion and a neutral M without fragmentation of the PIB backbones. However, structurally important product ions were produced from the corresponding [M + NH 4 ] + ions, allowing us to obtain valuable information on the arm-length distributions of the PIBs containing aromatic initiator moiety. In addition, a model was developed to interpret the oligomer distributions and the number average molecular weights observed in DART-MS for PIBs and other polymers of low molecular weight. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

Contextual interference effects on the acquisition of skill and strength of the bench press.

PubMed

Naimo, Marshall A; Zourdos, Michael C; Wilson, Jacob M; Kim, Jeong-Su; Ward, Emery G; Eccles, David W; Panton, Lynn B

2013-06-01

The purpose of this study was to investigate contextual interference effects on skill acquisition and strength gains during the learning of the bench press movement. Twenty-four healthy, college-aged males and females were stratified to control, high contextual interference (HCI), and low contextual interference (LCI) groups. Treatment groups were provided with written and visual instruction on proper bench press form and practiced the bench press and dart throwing for four weeks. Within each session, LCI performed all bench press sets before undertaking dart-throws. HCI undertook dart-throws immediately following each set of bench press. Control only did testing. Measurements, including one repetition maximum (1RM), checklist scores based on video recordings of participants' 1RM's, and dart-throw test scores were taken at pre-test, 1 week, 2 week, post-test, and retention test. Results were consistent with the basic premise of the contextual interference effect. LCI had significant improvements in percent 1RM and checklist scores during training, but were mostly absent after training (post-test and retention test). HCI had significant improvements in percent 1RM and checklist scores both during and after training. Thus, HCI may augment strength and movement skill on the bench press since proper technique is an important component of resistance exercise movements. Copyright © 2013 Elsevier B.V. All rights reserved.

The properties of SIRT, TVM, and DART for 3D imaging of tubular domains in nanocomposite thin-films and sections.

PubMed

Chen, Delei; Goris, Bart; Bleichrodt, Folkert; Mezerji, Hamed Heidari; Bals, Sara; Batenburg, Kees Joost; de With, Gijsbertus; Friedrich, Heiner

2014-12-01

In electron tomography, the fidelity of the 3D reconstruction strongly depends on the employed reconstruction algorithm. In this paper, the properties of SIRT, TVM and DART reconstructions are studied with respect to having only a limited number of electrons available for imaging and applying different angular sampling schemes. A well-defined realistic model is generated, which consists of tubular domains within a matrix having slab-geometry. Subsequently, the electron tomography workflow is simulated from calculated tilt-series over experimental effects to reconstruction. In comparison with the model, the fidelity of each reconstruction method is evaluated qualitatively and quantitatively based on global and local edge profiles and resolvable distance between particles. Results show that the performance of all reconstruction methods declines with the total electron dose. Overall, SIRT algorithm is the most stable method and insensitive to changes in angular sampling. TVM algorithm yields significantly sharper edges in the reconstruction, but the edge positions are strongly influenced by the tilt scheme and the tubular objects become thinned. The DART algorithm markedly suppresses the elongation artifacts along the beam direction and moreover segments the reconstruction which can be considered a significant advantage for quantification. Finally, no advantage of TVM and DART to deal better with fewer projections was observed. Copyright © 2014 Elsevier B.V. All rights reserved.

A crossbow suicide.

PubMed

Panata, Laura; Lancia, Massimo; Persichini, Alessandra; Scalise Pantuso, Sergio; Bacci, Mauro

2017-12-01

The crossbow is an ancient ranged weapon originally conceived for war and hunting. Although nowadays its use in warfare has been surpassed by firearms, it continues to be used in hunting, sports and recreation. The authors present the case of a 40-year-old man who suffered from severe depression. When his condition further deteriorated, doctors ordered a forced hospitalization but, just a few hours before the measure became effective, the man committed suicide using a crossbow. The autopsy and police investigation showed possession of the crossbow darts which the man used to shoot himself in the head. The forensic pathologist found the dart stuck in the skull: the entry wound was in the suprahyoid region while the arrow tip emerged in the left parietal region meaning the arrow crossed the tongue, the middle fossa, the brain and the cranial wall. All the wounds presented a three-pointed star shape consistent with the three sharp blades of the dart. An extensive blood infiltration affected the subdural and subarachnoid space, particularly where the dart had passed. The severe brain injury, extensive subdural and subarachnoid bleeding and brain swelling following the trauma caused the death. Even though the use of the crossbow is only permitted in sporting/hunting contexts, the reported case highlights the sharp contrast between its potential for harm and the easy access to this kind of weapon, even for those affected by mental illness. Copyright © 2017 Elsevier B.V. All rights reserved.

Detection of Nonvolatile Inorganic Oxidizer-Based Explosives from Wipe Collections by Infrared Thermal Desorption-Direct Analysis in Real Time Mass Spectrometry.

PubMed

Forbes, Thomas P; Sisco, Edward; Staymates, Matthew

2018-05-07

Infrared thermal desorption (IRTD) was coupled with direct analysis in real time mass spectrometry (DART-MS) for the detection of both inorganic and organic explosives from wipe collected samples. This platform generated discrete and rapid heating rates that allowed volatile and semivolatile organic explosives to thermally desorb at relatively lower temperatures, while still achieving elevated temperatures required to desorb nonvolatile inorganic oxidizer-based explosives. IRTD-DART-MS demonstrated the thermal desorption and detection of refractory potassium chlorate and potassium perchlorate oxidizers, compounds difficult to desorb with traditional moderate-temperature resistance-based thermal desorbers. Nanogram to sub-nanogram sensitivities were established for analysis of a range of organic and inorganic oxidizer-based explosive compounds, with further enhancement limited by the thermal properties of the most common commercial wipe materials. Detailed investigations and high-speed visualization revealed conduction from the heated glass-mica base plate as the dominant process for heating of the wipe and analyte materials, resulting in thermal desorption through boiling, aerosolization, and vaporization of samples. The thermal desorption and ionization characteristics of the IRTD-DART technique resulted in optimal sensitivity for the formation of nitrate adducts with both organic and inorganic species. The IRTD-DART-MS coupling and IRTD in general offer promising explosive detection capabilities to the defense, security, and law enforcement arenas.

Development of an Ultraflex-Based Thin Film Solar Array for Space Applications

NASA Technical Reports Server (NTRS)

White, Steve; Douglas, Mark; Spence, Brian; Jones, P. Alan; Piszczor, Michael F.

2003-01-01

As flexible thin film photovoltaic (FTFPV) cell technology is developed for space applications, integration into a viable solar array structure that optimizes the attributes of this cell technology is critical. An advanced version of ABLE'sS UltraFlex solar array platform represents a near-term, low-risk approach to demonstrating outstanding array performance with the implementation of FTFPV technology. Recent studies indicate that an advanced UltraFlex solar array populated with 15% efficient thin film cells can achieve over 200 W/kg EOL. An overview on the status of hardware development and the future potential of this technology is presented.

Rapid analysis of Δ-9-tetrahydrocannabinol in hair using direct analysis in real time ambient ionization orbitrap mass spectrometry.

PubMed

Duvivier, Wilco F; van Beek, Teris A; Pennings, Ed J M; Nielen, Michel W F

2014-04-15

Forensic hair analysis methods are laborious, time-consuming and provide only a rough retrospective estimate of the time of drug intake. Recently, hair imaging methods using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) were reported, but these methods require the application of MALDI matrix and are performed under vacuum. Direct analysis of entire locks of hair without any sample pretreatment and with improved spatial resolution would thus address a need. Hair samples were attached to stainless steel mesh screens and scanned in the X-direction using direct analysis in real time (DART) ambient ionization orbitrap MS. The DART gas temperature and the accuracy of the probed hair zone were optimized using Δ-9-tetrahydrocannabinol (THC) as a model compound. Since external contamination is a major issue in forensic hair analysis, sub-samples were measured before and after dichloromethane decontamination. The relative intensity of the THC signal in spiked blank hair versus that of quinine as the internal standard showed good reproducibility (26% RSD) and linearity of the method (R(2)  = 0.991). With the DART hair scan THC could be detected in hair samples from different chronic cannabis users. The presence of THC was confirmed by quantitative liquid chromatography/tandem mass spectrometry. Zones with different THC content could be clearly distinguished, indicating that the method might be used for retrospective timeline assessments. Detection of THC in decontaminated drug user hair showed that the DART hair scan not only probes THC on the surface of hair, but penetrates deeply enough to measure incorporated THC. A new approach in forensic hair analysis has been developed by probing complete locks of hair using DART-MS. Longitudinal scanning enables detection of incorporated compounds and can be used as pre-screening for THC without sample preparation. The method could also be adjusted for the analysis of other drugs of abuse. Copyright © 2014 John Wiley & Sons, Ltd.

Simulated Radioscapholunate Fusion Alters Carpal Kinematics While Preserving Dart-Thrower's Motion

PubMed Central

Calfee, Ryan P.; Leventhal, Evan L.; Wilkerson, Jim; Moore, Douglas C.; Akelman, Edward; Crisco, Joseph J.

2014-01-01

Purpose Midcarpal degeneration is well documented after radioscapholunate fusion. This study tested the hypothesis that radioscapholunate fusion alters the kinematic behavior of the remaining lunotriquetral and midcarpal joints, with specific focus on the dart-thrower's motion. Methods Simulated radioscapholunate fusions were performed on 6 cadaveric wrists in an anatomically neutral posture. Two 0.060-in. carbon fiber pins were placed from proximal to distal across the radiolunate and radioscaphoid joints, respectively. The wrists were passively positioned in a custom jig toward a full range of motion along the orthogonal axes as well as oblique motions, with additional intermediate positions along the dart-thrower's path. Using a computed tomography– based markerless bone registration technique, each carpal bone's three-dimensional rotation was defined as a function of wrist flexion/extension from the pinned neutral position. Kinematic data was analyzed against data collected on the same wrist prior to fixation using hierarchical linear regression analysis and paired Student's t-tests. Results After simulated fusion, wrist motion was restricted to an average flexion-extension arc of 48°, reduced from 77°, and radial-ulnar deviation arc of 19°, reduced from 33°. The remaining motion was maximally preserved along the dart-thrower's path from radial-extension toward ulnar-flexion. The simulated fusion significantly increased rotation through the scaphotrapezial joint, scaphocapitate joint, triquetrohamate joint, and lunotriquetral joint. For example, in the pinned wrist, the rotation of the hamate relative to the triquetrum increased 85%. Therefore, during every 10° of total wrist motion, the hamate rotated an average of nearly 8° relative to the triquetrum after pinning versus 4° in the normal state. Conclusions Simulated radioscapholunate fusion altered midcarpal and lunotriquetral kinematics. The increased rotations across these remaining joints provide one potential explanation for midcarpal degeneration after radioscapholunate fusion. Additionally, this fusion model confirms the dart-thrower's hypothesis, as wrist motion after simulated radioscapholunate fusion was primarily preserved from radial-extension toward ulnar-flexion. PMID:18406953

Principal component directed partial least squares analysis for combining nuclear magnetic resonance and mass spectrometry data in metabolomics: application to the detection of breast cancer.

PubMed

Gu, Haiwei; Pan, Zhengzheng; Xi, Bowei; Asiago, Vincent; Musselman, Brian; Raftery, Daniel

2011-02-07

Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) are the two most commonly used analytical tools in metabolomics, and their complementary nature makes the combination particularly attractive. A combined analytical approach can improve the potential for providing reliable methods to detect metabolic profile alterations in biofluids or tissues caused by disease, toxicity, etc. In this paper, (1)H NMR spectroscopy and direct analysis in real time (DART)-MS were used for the metabolomics analysis of serum samples from breast cancer patients and healthy controls. Principal component analysis (PCA) of the NMR data showed that the first principal component (PC1) scores could be used to separate cancer from normal samples. However, no such obvious clustering could be observed in the PCA score plot of DART-MS data, even though DART-MS can provide a rich and informative metabolic profile. Using a modified multivariate statistical approach, the DART-MS data were then reevaluated by orthogonal signal correction (OSC) pretreated partial least squares (PLS), in which the Y matrix in the regression was set to the PC1 score values from the NMR data analysis. This approach, and a similar one using the first latent variable from PLS-DA of the NMR data resulted in a significant improvement of the separation between the disease samples and normals, and a metabolic profile related to breast cancer could be extracted from DART-MS. The new approach allows the disease classification to be expressed on a continuum as opposed to a binary scale and thus better represents the disease and healthy classifications. An improved metabolic profile obtained by combining MS and NMR by this approach may be useful to achieve more accurate disease detection and gain more insight regarding disease mechanisms and biology. Copyright © 2010 Elsevier B.V. All rights reserved.

Asteroid Impact & Deflection Assessment mission: Kinetic impactor

NASA Astrophysics Data System (ADS)

Cheng, A. F.; Michel, P.; Jutzi, M.; Rivkin, A. S.; Stickle, A.; Barnouin, O.; Ernst, C.; Atchison, J.; Pravec, P.; Richardson, D. C.; AIDA Team

2016-02-01

The Asteroid Impact & Deflection Assessment (AIDA) mission will be the first space experiment to demonstrate asteroid impact hazard mitigation by using a kinetic impactor to deflect an asteroid. AIDA is an international cooperation, consisting of two mission elements: the NASA Double Asteroid Redirection Test (DART) mission and the ESA Asteroid Impact Mission (AIM) rendezvous mission. The primary goals of AIDA are (i) to test our ability to perform a spacecraft impact on a potentially hazardous near-Earth asteroid and (ii) to measure and characterize the deflection caused by the impact. The AIDA target will be the binary near-Earth asteroid (65803) Didymos, with the deflection experiment to occur in late September, 2022. The DART impact on the secondary member of the binary at 7 km/s is expected to alter the binary orbit period by about 4 minutes, assuming a simple transfer of momentum to the target, and this period change will be measured by Earth-based observatories. The AIM spacecraft will characterize the asteroid target and monitor results of the impact in situ at Didymos. The DART mission is a full-scale kinetic impact to deflect a 150 m diameter asteroid, with known impactor conditions and with target physical properties characterized by the AIM mission. Predictions for the momentum transfer efficiency of kinetic impacts are given for several possible target types of different porosities, using Housen and Holsapple (2011) crater scaling model for impact ejecta mass and velocity distributions. Results are compared to numerical simulation results using the Smoothed Particle Hydrodynamics code of Jutzi and Michel (2014) with good agreement. The model also predicts that the ejecta from the DART impact may make Didymos into an active asteroid, forming an ejecta coma that may be observable from Earth-based telescopes. The measurements from AIDA of the momentum transfer from the DART impact, the crater size and morphology, and the evolution of an ejecta coma will substantially advance understanding of impact processes on asteroids.

Discrete tomography in an in vivo small animal bone study.

PubMed

Van de Casteele, Elke; Perilli, Egon; Van Aarle, Wim; Reynolds, Karen J; Sijbers, Jan

2018-01-01

This study aimed at assessing the feasibility of a discrete algebraic reconstruction technique (DART) to be used in in vivo small animal bone studies. The advantage of discrete tomography is the possibility to reduce the amount of X-ray projection images, which makes scans faster and implies also a significant reduction of radiation dose, without compromising the reconstruction results. Bone studies are ideal for being performed with discrete tomography, due to the relatively small number of attenuation coefficients contained in the image [namely three: background (air), soft tissue and bone]. In this paper, a validation is made by comparing trabecular bone morphometric parameters calculated from images obtained by using DART and the commonly used standard filtered back-projection (FBP). Female rats were divided into an ovariectomized (OVX) and a sham-operated group. In vivo micro-CT scanning of the tibia was done at baseline and at 2, 4, 8 and 12 weeks after surgery. The cross-section images were reconstructed using first the full set of projection images and afterwards reducing them in number to a quarter and one-sixth (248, 62, 42 projection images, respectively). For both reconstruction methods, similar changes in morphometric parameters were observed over time: bone loss for OVX and bone growth for sham-operated rats, although for DART the actual values were systematically higher (bone volume fraction) or lower (structure model index) compared to FBP, depending on the morphometric parameter. The DART algorithm was, however, more robust when using fewer projection images, where the standard FBP reconstruction was more prone to noise, showing a significantly bigger deviation from the morphometric parameters obtained using all projection images. This study supports the use of DART as a potential alternative method to FBP in X-ray micro-CT animal studies, in particular, when the number of projections has to be drastically minimized, which directly reduces scanning time and dose.

Evaluation of medetomidine-ketamine and atipamezole for reversible anesthesia of free-ranging gray wolves (Canis lupus).

PubMed

Arnemo, Jon M; Evans, Alina L; Ahlqvist, Per; Segerström, Peter; Liberg, Olof

2013-04-01

Twenty-eight anesthetic events were carried out on 24 free-ranging Scandinavian gray wolves (Canis lupus) by darting from a helicopter with 5 mg medetomidine and 250 mg ketamine during winter in 2002 and 2003. Mean±SD doses were 0.162±0.008 mg medetomidine/kg and 8.1±0.4 mg ketamine/kg in juveniles (7-10 mo old) and 0.110±0.014 mg medetomidine/kg and 5.7±0.5 mg ketamine/kg in adults (>19 mo old). Mean±SD induction time was shorter (P<0.01) in juveniles (2.3±0.8 min) than in adults (4.1±0.6 min). In 26 cases, the animals were completely immobilized after one dart. Muscle relaxation was good, palpebral reflexes were present, and there were no reactions to handling or minor painful stimuli. Mild to severe hyperthermia was detected in 14/28 anesthetic events. Atipamezole (5 mg per mg medetomidine) was injected intramuscularly for reversal 98±28 and 94±40 min after darting in juveniles and adults, respectively. Mean±SD time from administration of atipamezole to coordinated walking was 38±20 min in juveniles and 41±21 min in adults. Recovery was uneventful in 25 anesthetic events, although vomiting was observed in five animals. One adult that did not respond to atipamezole was given intravenous fluids and was fully recovered 8 hr after darting. Two animals died 7-9 hr after capture, despite intensive care. Both mortalities were attributed to shock and circulatory collapse following stress-induced hyperthermia. Although effective, this combination cannot be recommended for darting free-ranging wolves from helicopter at the doses presented here because of the severe hyperthermia seen in several wolves, two deaths, and prolonged recovery in one individual.

Generic DART-MS platform for monitoring the on-demand continuous-flow production of pharmaceuticals: Advancing the quantitative protocol for caffeates in microfluidic biocatalysis.

PubMed

Xu, Yan; Zhang, Dong-Yang; Meng, Xiang-Yun; Liu, Xi; Sheng, Sheng; Wu, Guo-Hua; Wang, Jun; Wu, Fu-An

2017-04-15

Today, continuous processing is regarded as an effective on-demand production technique of pharmaceuticals. Homemade microreactors packed with immobilized lipase under continuous-flow conditions were first applied to tailor the production of high-value caffeic acid phenethyl ester (CAPE) from methyl caffeate (MC) and 2-phenylethanol (PE) in cyclohexane via transesterification; however, this method is challenging due to the lack of a rapid platform for monitoring caffeates in microfluidic biocatalysis. The reactants were directly analyzed using Direct Analysis in Real Time Mass Spectrometry (DART-MS), and the corresponding ionization parameters were investigated. Special ions produced from MC (parent ion m/z 192.87 and product ion m/z 133.44) and CAPE (parent ion m/z 282.93 and product ion m/z 178.87) were determined using DART-MS 2 in the negative ion mode. The peak areas of the select reaction monitoring (SRM) signals were calculated to develop the standard curves for quantitative analyses of the concentration. Reasonable linear regression equations of MC and CAPE were obtained in the range of 3.125-50.000mg/L, with linear coefficients (R 2 ) of 0.9515 and 0.9973, limits of detection (LOD) of 0.005 and 0.003mg/L, limits of quantification (LOQ) of 0.02 and 0.01mg/L, and recovery ranges of 92.50-97.11% and 90.11-97.60%, respectively. The results using DART-MS 2 were in good agreement with those using conventional High-Performance Liquid Chromatography with a UV detector (HPLC-UV) and were successfully applied to monitor the kinetics constants and mass transfer coefficients in a continuous-flow packed bed microreactor. Thus, the DART-MS 2 method is an efficient tool for analyzing caffeates in microfluidic biocatalysis with limited sample preparation and short operating time. Copyright © 2017 Elsevier B.V. All rights reserved.

Genomic Regions Influencing Seminal Root Traits in Barley.

PubMed

Robinson, Hannah; Hickey, Lee; Richard, Cecile; Mace, Emma; Kelly, Alison; Borrell, Andrew; Franckowiak, Jerome; Fox, Glen

2016-03-01

Water availability is a major limiting factor for crop production, making drought adaptation and its many component traits a desirable attribute of plant cultivars. Previous studies in cereal crops indicate that root traits expressed at early plant developmental stages, such as seminal root angle and root number, are associated with water extraction at different depths. Here, we conducted the first study to map seminal root traits in barley ( L.). Using a recently developed high-throughput phenotyping method, a panel of 30 barley genotypes and a doubled-haploid (DH) population (ND24260 × 'Flagship') comprising 330 lines genotyped with diversity array technology (DArT) markers were evaluated for seminal root angle (deviation from vertical) and root number under controlled environmental conditions. A high degree of phenotypic variation was observed in the panel of 30 genotypes: 13.5 to 82.2 and 3.6 to 6.9° for root angle and root number, respectively. A similar range was observed in the DH population: 16.4 to 70.5 and 3.6 to 6.5° for root angle and number, respectively. Seven quantitative trait loci (QTL) for seminal root traits (root angle, two QTL; root number, five QTL) were detected in the DH population. A major QTL influencing both root angle and root number (/) was positioned on chromosome 5HL. Across-species analysis identified 10 common genes underlying root trait QTL in barley, wheat ( L.), and sorghum [ (L.) Moench]. Here, we provide insight into seminal root phenotypes and provide a first look at the genetics controlling these traits in barley. Copyright © 2016 Crop Science Society of America.

Comparative Genomics Analyses Reveal Extensive Chromosome Colinearity and Novel Quantitative Trait Loci in Eucalyptus.

PubMed

Li, Fagen; Zhou, Changpin; Weng, Qijie; Li, Mei; Yu, Xiaoli; Guo, Yong; Wang, Yu; Zhang, Xiaohong; Gan, Siming

2015-01-01

Dense genetic maps, along with quantitative trait loci (QTLs) detected on such maps, are powerful tools for genomics and molecular breeding studies. In the important woody genus Eucalyptus, the recent release of E. grandis genome sequence allows for sequence-based genomic comparison and searching for positional candidate genes within QTL regions. Here, dense genetic maps were constructed for E. urophylla and E. tereticornis using genomic simple sequence repeats (SSR), expressed sequence tag (EST) derived SSR, EST-derived cleaved amplified polymorphic sequence (EST-CAPS), and diversity arrays technology (DArT) markers. The E. urophylla and E. tereticornis maps comprised 700 and 585 markers across 11 linkage groups, totaling at 1,208.2 and 1,241.4 cM in length, respectively. Extensive synteny and colinearity were observed as compared to three earlier DArT-based eucalypt maps (two maps with E. grandis × E. urophylla and one map of E. globulus) and with the E. grandis genome sequence. Fifty-three QTLs for growth (10-56 months of age) and wood density (56 months) were identified in 22 discrete regions on both maps, in which only one colocalizaiton was found between growth and wood density. Novel QTLs were revealed as compared with those previously detected on DArT-based maps for similar ages in Eucalyptus. Eleven to 585 positional candidate genes were obained for a 56-month-old QTL through aligning QTL confidence interval with the E. grandis genome. These results will assist in comparative genomics studies, targeted gene characterization, and marker-assisted selection in Eucalyptus and the related taxa.

Comparative Genomics Analyses Reveal Extensive Chromosome Colinearity and Novel Quantitative Trait Loci in Eucalyptus

PubMed Central

Weng, Qijie; Li, Mei; Yu, Xiaoli; Guo, Yong; Wang, Yu; Zhang, Xiaohong; Gan, Siming

2015-01-01

Dense genetic maps, along with quantitative trait loci (QTLs) detected on such maps, are powerful tools for genomics and molecular breeding studies. In the important woody genus Eucalyptus, the recent release of E. grandis genome sequence allows for sequence-based genomic comparison and searching for positional candidate genes within QTL regions. Here, dense genetic maps were constructed for E. urophylla and E. tereticornis using genomic simple sequence repeats (SSR), expressed sequence tag (EST) derived SSR, EST-derived cleaved amplified polymorphic sequence (EST-CAPS), and diversity arrays technology (DArT) markers. The E. urophylla and E. tereticornis maps comprised 700 and 585 markers across 11 linkage groups, totaling at 1,208.2 and 1,241.4 cM in length, respectively. Extensive synteny and colinearity were observed as compared to three earlier DArT-based eucalypt maps (two maps with E. grandis × E. urophylla and one map of E. globulus) and with the E. grandis genome sequence. Fifty-three QTLs for growth (10–56 months of age) and wood density (56 months) were identified in 22 discrete regions on both maps, in which only one colocalizaiton was found between growth and wood density. Novel QTLs were revealed as compared with those previously detected on DArT-based maps for similar ages in Eucalyptus. Eleven to 585 positional candidate genes were obained for a 56-month-old QTL through aligning QTL confidence interval with the E. grandis genome. These results will assist in comparative genomics studies, targeted gene characterization, and marker-assisted selection in Eucalyptus and the related taxa. PMID:26695430

Determination of drugs and drug-like compounds in different samples with direct analysis in real time mass spectrometry.

PubMed

Chernetsova, Elena S; Morlock, Gertrud E

2011-01-01

Direct analysis in real time (DART), a relatively new ionization source for mass spectrometry, ionizes small-molecule components from different kinds of samples without any sample preparation and chromatographic separation. The current paper reviews the published data available on the determination of drugs and drug-like compounds in different matrices with DART-MS, including identification and quantitation issues. Parameters that affect ionization efficiency and mass spectra composition are also discussed. Copyright © 2011 Wiley Periodicals, Inc.

Access to CAMAC from VxWorks and UNIX in DART

NASA Astrophysics Data System (ADS)

Streets, J.; Meadows, J.; Moore, C.; Pordes, R.; Slimmer, D.; Vittone, M.; Stern, E.

1996-02-01

As part of the DART Project [Data acquisition for the next Generation Fermilab Fixed Target Experiments] we have developed a package of software for CAMAC access from UNIX and VxWorks platforms, with support for several hardware interfaces. We report on developments for the CES CBD8210 VME to parallel CAMAC, the Hytec VSD2992 VME to serial CAMAC and Jorway 411s SCSI to parallel and serial CAMAC branch drivers, and give a summary of the timings obtained.

DART Support for Hurricane Matthew

NASA Image and Video Library

2016-10-13

Members of the Disaster Assessment and Recovery Team (DART) repair a section of roof atop the Operations Support Building II at NASA’s Kennedy Space Center in Florida. The effort is part of the spaceport’s ongoing recovery from Hurricane Matthew, which passed to the east of Kennedy on Oct. 6 and 7, 2016. The center received some isolated roof damage, damaged support buildings, a few downed power lines, and limited water intrusion. Beach erosion also occurred, although the storm surge was less than expected.

Novel use of cutting balloon to treat subintimal hematomas during chronic total occlusion interventions.

PubMed

Vo, Minh N; Brilakis, Emmanouil S; Grantham, J Aaron

2018-01-01

Contemporary chronic total occlusion (CTO) percutaneous coronary interventional (PCI) techniques are increasingly dependent upon dissection and reentry techniques (DARTs) especially for long occluded lesions. DARTs can result in compressive hematomas during CTO interventions and traditional treatment with balloon angioplasty and/or coronary stenting are often suboptimal and may extend the hematoma distally. We describe the novel use of a cutting balloon to "express" these compressive hematomas and restore antegrade coronary blood flow. © 2017 Wiley Periodicals, Inc.

Radio-triggered anesthetic-dart collar for recapturing large mammals

USGS Publications Warehouse

Mech, L.D.; Chapman, R.C.; Cochran, W.W.; Simmons, L.; Seal, U.S.

1984-01-01

A re-usable, radio-triggered, anesthetic-dart collar costing about $1,200 has been developed for recapturing large, free-ranging mammals. The collar was tested successfully on captive gray wolves, white-tailed deer, a mountain lion, a black bear, and a tiger, and on wild wolves, a bear, and deer after periods of up to 32 days. The main advantage of the capture collar is that it allows frequent recapture of individuals at will and thus enhances opportunities for new physiological monitoring of wild animals.

DART Support for Hurricane Matthew

NASA Image and Video Library

2016-10-13

Tubing provides ventilation through boarded-up windows on the Operations Support Building II at NASA’s Kennedy Space Center in Florida. Members of the Disaster Assessment and Recovery Team (DART) are working on repairs to the facility following Hurricane Matthew, which passed to the east of Kennedy on Oct. 6 and 7, 2016. The center received some isolated roof damage, damaged support buildings, a few downed power lines, and limited water intrusion. Beach erosion also occurred, although the storm surge was less than expected.

DART Support for Hurricane Matthew

NASA Image and Video Library

2016-10-13

Members of the Disaster Assessment and Recovery Team (DART) work on repairs to the Operations Support Building II at NASA’s Kennedy Space Center in Florida. The effort is part of the spaceport’s ongoing recovery from Hurricane Matthew, which passed to the east of Kennedy on Oct. 6 and 7, 2016. The center received some isolated roof damage, damaged support buildings, a few downed power lines, and limited water intrusion. Beach erosion also occurred, although the storm surge was less than expected.

DART Support for Hurricane Matthew

NASA Image and Video Library

2016-10-13

Roofing materials, blown loose by Hurricane Matthew, are visible on the ground below the deck of the Beach House at NASA’s Kennedy Space Center in Florida. Members of the Disaster Assessment and Recovery Team (DART) are working on repairs to the facility following Hurricane Matthew, which passed to the east of Kennedy on Oct. 6 and 7, 2016. The center received some isolated roof damage, damaged support buildings, a few downed power lines, and limited water intrusion. Beach erosion also occurred, although the storm surge was less than expected.

DART Support for Hurricane Matthew

NASA Image and Video Library

2016-10-13

Roofing materials, blown loose by Hurricane Matthew, lie on the ground behind the Beach House at NASA’s Kennedy Space Center in Florida. Members of the Disaster Assessment and Recovery Team (DART) are working on repairs to the facility following Hurricane Matthew, which passed to the east of Kennedy on Oct. 6 and 7, 2016. The center received some isolated roof damage, damaged support buildings, a few downed power lines, and limited water intrusion. Beach erosion also occurred, although the storm surge was less than expected.

Mass properties survey of solar array technologies

NASA Technical Reports Server (NTRS)

Kraus, Robert

1991-01-01

An overview of the technologies, electrical performance, and mass characteristics of many of the presently available and the more advanced developmental space solar array technologies is presented. Qualitative trends and quantitative mass estimates as total array output power is increased from 1 kW to 5 kW at End of Life (EOL) from a single wing are shown. The array technologies are part of a database supporting an ongoing solar power subsystem model development for top level subsystem and technology analyses. The model is used to estimate the overall electrical and thermal performance of the complete subsystem, and then calculate the mass and volume of the array, batteries, power management, and thermal control elements as an initial sizing. The array types considered here include planar rigid panel designs, flexible and rigid fold-out planar arrays, and two concentrator designs, one with one critical axis and the other with two critical axes. Solar cell technologies of Si, GaAs, and InP were included in the analyses. Comparisons were made at the array level; hinges, booms, harnesses, support structures, power transfer, and launch retention mountings were included. It is important to note that the results presented are approximations, and in some cases revised or modified performance and mass estimates of specific designs.

Advanced Video Guidance Sensor and next-generation autonomous docking sensors

NASA Astrophysics Data System (ADS)

Granade, Stephen R.

2004-09-01

In recent decades, NASA's interest in spacecraft rendezvous and proximity operations has grown. Additional instrumentation is needed to improve manned docking operations' safety, as well as to enable telerobotic operation of spacecraft or completely autonomous rendezvous and docking. To address this need, Advanced Optical Systems, Inc., Orbital Sciences Corporation, and Marshall Space Flight Center have developed the Advanced Video Guidance Sensor (AVGS) under the auspices of the Demonstration of Autonomous Rendezvous Technology (DART) program. Given a cooperative target comprising several retro-reflectors, AVGS provides six-degree-of-freedom information at ranges of up to 300 meters for the DART target. It does so by imaging the target, then performing pattern recognition on the resulting image. Longer range operation is possible through different target geometries. Now that AVGS is being readied for its test flight in 2004, the question is: what next? Modifications can be made to AVGS, including different pattern recognition algorithms and changes to the retro-reflector targets, to make it more robust and accurate. AVGS could be coupled with other space-qualified sensors, such as a laser range-and-bearing finder, that would operate at longer ranges. Different target configurations, including the use of active targets, could result in significant miniaturization over the current AVGS package. We will discuss these and other possibilities for a next-generation docking sensor or sensor suite that involve AVGS.

Advanced Video Guidance Sensor and Next Generation Autonomous Docking Sensors

NASA Technical Reports Server (NTRS)

Granade, Stephen R.

2004-01-01

In recent decades, NASA's interest in spacecraft rendezvous and proximity operations has grown. Additional instrumentation is needed to improve manned docking operations' safety, as well as to enable telerobotic operation of spacecraft or completely autonomous rendezvous and docking. To address this need, Advanced Optical Systems, Inc., Orbital Sciences Corporation, and Marshall Space Flight Center have developed the Advanced Video Guidance Sensor (AVGS) under the auspices of the Demonstration of Autonomous Rendezvous Technology (DART) program. Given a cooperative target comprising several retro-reflectors, AVGS provides six-degree-of-freedom information at ranges of up to 300 meters for the DART target. It does so by imaging the target, then performing pattern recognition on the resulting image. Longer range operation is possible through different target geometries. Now that AVGS is being readied for its test flight in 2004, the question is: what next? Modifications can be made to AVGS, including different pattern recognition algorithms and changes to the retro-reflector targets, to make it more robust and accurate. AVGS could be coupled with other space-qualified sensors, such as a laser range-and-bearing finder, that would operate at longer ranges. Different target configurations, including the use of active targets, could result in significant miniaturization over the current AVGS package. We will discuss these and other possibilities for a next-generation docking sensor or sensor suite that involve AVGS.

Novel approaches to analysis of 3-chloropropane-1,2-diol esters in vegetable oils.

PubMed

Moravcova, Eliska; Vaclavik, Lukas; Lacina, Ondrej; Hrbek, Vojtech; Riddellova, Katerina; Hajslova, Jana

2012-03-01

A sensitive and accurate method utilizing ultrahigh performance liquid chromatography (U-HPLC) coupled to high resolution mass spectrometry based on orbitrap technology (orbitrapMS) for the analysis of nine 3-chloropropane-1,2-diol (3-MCPD) diesters in vegetable oils was developed. To remove the interfering triacylglycerols that induce strong matrix effects, a clean-up step on silica gel column was used. The quantitative analysis was performed with the use of deuterium-labeled internal standards. The lowest calibration levels estimated for the respective analytes ranged from 2 to 5 μg kg(-1). Good recovery values (89-120%) and repeatability (RSD 5-9%) was obtained at spiking levels of 2 and 10 mg kg(-1). As an alternative, a novel ambient desorption ionization technique, direct analysis in real time (DART), hyphenated with orbitrapMS, was employed for no separation, high-throughput, semi-quantitative screening of 3-MCPD diesters in samples obtained by chromatographic fractionation. Additionally, the levels of 3-MCPD diesters measured in reallife vegetable oil samples (palm oil, sunflower oil, rapeseed oil) using both methods are reported. Relatively good agreement of the data generated by U-HPLC-orbitrapMS and DART-orbitrapMS were observed. With regard to a low ionization yield achieved for 3-MCPD monoesters, the methods presented in this paper were not yet applicable for the analysis of these contaminants at the naturally occurring levels.

Recent improvements in earthquake and tsunami monitoring in the Caribbean

NASA Astrophysics Data System (ADS)

Gee, L.; Green, D.; McNamara, D.; Whitmore, P.; Weaver, J.; Huang, P.; Benz, H.

2007-12-01

Following the catastrophic loss of life from the December 26, 2004, Sumatra-Andaman Islands earthquake and tsunami, the U.S. Government appropriated funds to improve monitoring along a major portion of vulnerable coastal regions in the Caribbean Sea, the Gulf of Mexico, and the Atlantic Ocean. Partners in this project include the United States Geological Survey (USGS), the National Oceanic and Atmospheric Administration (NOAA), the Puerto Rico Seismic Network (PRSN), the Seismic Research Unit of the University of the West Indies, and other collaborating institutions in the Caribbean region. As part of this effort, the USGS is coordinating with Caribbean host nations to design and deploy nine new broadband and strong-motion seismic stations. The instrumentation consists of an STS-2 seismometer, an Episensor accelerometer, and a Q330 high resolution digitizer. Six stations are currently transmitting data to the USGS National Earthquake Information Center, where the data are redistributed to the NOAA's Tsunami Warning Centers, regional monitoring partners, and the IRIS Data Management Center. Operating stations include: Isla Barro Colorado, Panama; Gun Hill Barbados; Grenville, Grenada; Guantanamo Bay, Cuba; Sabaneta Dam, Dominican Republic; and Tegucigalpa, Honduras. Three additional stations in Barbuda, Grand Turks, and Jamaica will be completed during the fall of 2007. These nine stations are affiliates of the Global Seismographic Network (GSN) and complement existing GSN stations as well as regional stations. The new seismic stations improve azimuthal coverage, increase network density, and provide on-scale recording throughout the region. Complementary to this network, NOAA has placed Deep-ocean Assessment and Reporting of Tsunami (DART) stations at sites in regions with a history of generating destructive tsunamis. Recently, NOAA completed deployment of 7 DART stations off the coasts of Montauk Pt, NY; Charleston, SC; Miami, FL; San Juan, Puerto Rico; New Orleans, LA; and Bermuda as part of the U.S. tsunami warning system expansion. DART systems consist of an anchored seafloor pressure recorder (BPR) and a companion moored surface buoy for real-time communications. The new stations are a second-generation design (DART II) equipped with two- way satellite communications that allow NOAA's Tsunami Warning Centers to set stations in event mode in anticipation of possible tsunamis or retrieve the high-resolution (15-s intervals) data in one-hour blocks for detailed analysis. Combined with development of sophisticated wave propagation and site-specific inundation models, the DART data are being used to forecast wave heights for at-risk coastal communities. NOAA expects to deploy a total of 39 DART II buoy stations by 2008 (32 in the Pacific and 7 in the Atlantic, Caribbean and Gulf regions). The seismic and DART networks are two components in a comprehensive and fully-operational global observing system to detect and warn the public of earthquake and tsunami threats. NOAA and USGS are working together to make important strides in enhancing communication networks so residents and visitors can receive earthquake and tsunami watches and warnings around the clock.

NASA advanced space photovoltaic technology-status, potential and future mission applications

NASA Technical Reports Server (NTRS)

Flood, Dennis J.; Piszczor, Michael, Jr.; Stella, Paul M.; Bennett, Gary L.

1989-01-01

The NASA program in space photovoltaic research and development encompasses a wide range of emerging options for future space power systems, and includes both cell and array technology development. The long range goals are to develop technology capable of achieving 300 W/kg for planar arrays, and 300 W/sq m for concentrator arrays. InP and GaAs planar and concentrator cell technologies are under investigation for their potential high efficiency and good radiation resistance. The Advanced Photovoltaic Solar Array (APSA) program is a near term effort aimed at demonstrating 130 W/kg beginning of life specific power using thin (62 micrometer) silicon cells. It is intended to be technology transparent to future high efficiency cells and provides the baseline for development of the 300 W/kg array.

Quantification of Endogenous Cholesterol in Human Serum on Paper Using Direct Analysis in Real Time Mass Spectrometry.

PubMed

Hsieh, Hua-Yi; Li, Li-Hua; Hsu, Ren-Yu; Kao, Wei-Fong; Huang, Ying-Chen; Hsu, Cheng-Chih

2017-06-06

Blood testing for endogenous small metabolites to determine physiological and biochemical states is routine for laboratory analysis. Here we demonstrate that by combining the commercial direct analysis in real time (DART) ion source with an ion trap mass spectrometer, native cholesterol in its free alcohol form is readily detected from a few hundred nanoliters of human serum loaded onto chromatography paper. Deuterium-labeled cholesterol was used as the internal standard to obtain the absolute quantity of the endogenous cholesterol. The amount of the cholesterol measured by this paper-loaded DART mass spectrometry (pDART-MS) is statistically comparable with that obtained by using commercially available fluorometric-enzymatic assay and liquid chromatography/mass spectrometry. Furthermore, sera from 21 participants at three different time points in an ultramarathon were collected to obtain their cholesterol levels. The test requires only very minimal sample preparation, and the concentrations of cholesterol in each sample were acquired within a minute.

Rapid Identification of Synthetic Cannabinoids in Herbal Incenses with DART-MS and NMR.

PubMed

Marino, Michael A; Voyer, Brandy; Cody, Robert B; Dane, A John; Veltri, Mercurio; Huang, Ling

2016-01-01

The usage of herbal incenses containing synthetic cannabinoids has caused an increase in medical incidents and triggered legislations to ban these products throughout the world. Law enforcement agencies are experiencing sample backlogs due to the variety of the products and the addition of new and still-legal compounds. In our study, proton nuclear magnetic resonance (NMR) spectroscopy was employed to promptly screen the synthetic cannabinoids after their rapid, direct detection on the herbs and in the powders by direct analysis in real time mass spectrometry (DART-MS). A simple sample preparation protocol was employed on 50 mg of herbal sample matrices for quick NMR detection. Ten synthetic cannabinoids were discovered in fifteen herbal incenses. The combined DART-MS and NMR methods can be used to quickly screen synthetic cannabinoids in powder and herbal samples, serving as a complementary approach to conventional GC-MS or LC-MS methods. © 2015 American Academy of Forensic Sciences.

DART - LTQ ORBITRAP as an expedient tool for the identification of synthetic cannabinoids.

PubMed

Habala, Ladislav; Valentová, Jindra; Pechová, Iveta; Fuknová, Mária; Devínsky, Ferdinand

2016-05-01

Synthetic cannabinoids as designer drugs constitute a major problem due to their rapid increase in number and the difficulties connected with their identification in complex mixtures. DART (Direct Analysis in Real Time) has emerged as an advantageous tool for the direct and rapid analysis of complex samples by mass spectrometry. Here we report on the identification of six synthetic cannabinoids originating from seized material in various matrices, employing the combination of ambient pressure ion source DART and hybrid ion trap - LTQ ORBITRAP mass spectrometer. This report also describes the sampling techniques for the provided herbal material containing the cannabinoids, either directly as plant parts or as an extract in methanol and their influence on the outcome of the analysis. The high resolution mass spectra supplied by the LTQ ORBITRAP instrument allowed for an unambiguous assignment of target compounds. The utilized instrumental coupling proved to be a convenient way for the identification of synthetic cannabinoids in real-world samples. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Detection of Low Molecular Weight Adulterants in Beverages by Direct Analysis in Real Time Mass Spectrometry.

PubMed

Sisco, Edward; Dake, Jeffrey

2016-04-14

Direct Analysis in Real Time Mass Spectrometry (DART-MS) has been used to detect the presence of non-narcotic adulterants in beverages. The non-narcotic adulterants that were examined in this work incorporated a number low molecular weight alcohols, acetone, ammonium hydroxide, and sodium hypochlorite. Analysis of the adulterants was completed by pipetting 1 µL deposits onto glass microcapillaries along with an appropriate dopant species followed by introduction into the DART gas stream. It was found that detection of these compounds in the complex matrices of common beverages (soda, energy drinks, etc.) was simplified through the use of a dopant species to allow for adduct formation with the desired compound(s) of interest. Other parameters that were investigated included DART gas stream temperature, in source collision induced dissociation, ion polarity, and DART needle voltage. Sensitivities of the technique were found to range from 0.001 % volume fraction to 0.1 % volume fraction, comparable to traditional analyses completed using headspace gas chromatography mass spectrometry (HS-GC/MS). Once a method was established using aqueous solutions, , fifteen beverages were spiked with each of the nine adulterants, to simulate real world detection, and in nearly all cases the adulterant could be detected either in pure form, or complexed with the added dopant species. This technique provides a rapid way to directly analyze beverages believed to be contaminated with non-narcotic adulterants at sensitivities similar to or exceeding those of traditional confirmatory analyses.

AIDA: The Asteroid Impact & Deflection Assessment Mission

NASA Astrophysics Data System (ADS)

Galvez, A.; Carnelli, I.; Michel, P.; Cheng, A. F.; Reed, C.; Ulamec, S.; Biele, J.; Abell, P.; Landis, R.

2013-09-01

The Asteroid Impact and Deflection Assessment (AIDA) mission, a joint effort of ESA, JHU/APL, NASA, OCA, and DLR, is the first demonstration of asteroid deflection and assessment via kinetic impact. AIDA consists of two independent but mutually supporting mission elements, one of which is the asteroid kinetic impactor and the other is the characterization spacecraft. These two missions are, respectively, JHU/APL's Double Asteroid Redirection Test (DART) and the European Space Agency's Asteroid Investigation Mission (AIM) missions. As in the separate DART and AIM studies, the target of this mission is the binary asteroid [65803] Didymos in October, 2022. For a successful joint mission, one spacecraft, DART, would impact the secondary of the Didymos system while AIM would observe and measure any change in the relative orbit. AIM will be the first probe to characterise a binary asteroid, especially from the dynamical point of view, but also considering its interior and subsurface composition. The mission concept focuses on the monitoring aspects i.e., the capability to determine in-situ the key physical properties of a binary asteroid playing a role in the system's dynamic behavior. DART will be the first ever space mission to deflect the trajectory of an asteroid in a measurable way.- It is expected that the deflection can be measured as a change in the relative orbit period with a precision better than 10%. The joint AIDA mission will return vital data to determine the momentum transfer efficiency of the kinetic impact [1,2].

Carfentanil and xylazine for immobilization of moose (Alces alces) on Isle Royale.

PubMed

Seal, U S; Schmitt, S M; Peterson, R O

1985-01-01

Twenty-one moose were captured on Isle Royale between 28 May and 2 June 1984 at licks on the western end of the island. The animals were darted, at ranges of 10 to 35 m, with 3-cc dart syringes containing 3 or 4 mg of carfentanil and 100 or 175 mg of xylazine. Three animals were drugged with carfentanil alone. Immobilization time ranged from 2.5 to 6 min. There was no excitement phase evident in 18 of the animals and they rarely moved more than 30 m after darting. Reversal was begun at 30 to 90 min after darting using naloxone and diprenorphine given intramuscularly (i.m.) and intravenously (i.v.). Recovery time varied from 10 min to 3 hr with the longer times occurring at the higher doses of immobilizing drugs. Two animals died within 30 hr, one as the result of aspiration of rumen contents and the other was unable to get on its feet and was euthanized. Analysis of blood samples from the 18 moose immobilized with the drug combination yielded hemoglobin values of 13.1 +/- 0.3 g/dl, hematocrit: 37.6 +/- 0.7%, red blood cells: 5.46 +/- 0.1 million/microliter, leucocytes: 6.1 +/- 0.4 thousand/microliter, and serum urea nitrogen: 29.3 +/- 1.6 mg/dl. Our experience indicated that quiet, undisturbed moose can be immobilized with 3 mg carfentanil and 100 mg or less of xylazine.

Solar Cell and Array Technology Development for NASA Solar Electric Propulsion Missions

NASA Technical Reports Server (NTRS)

Piszczor, Michael; McNatt, Jeremiah; Mercer, Carolyn; Kerslake, Tom; Pappa, Richard

2012-01-01

NASA is currently developing advanced solar cell and solar array technologies to support future exploration activities. These advanced photovoltaic technology development efforts are needed to enable very large (multi-hundred kilowatt) power systems that must be compatible with solar electric propulsion (SEP) missions. The technology being developed must address a wide variety of requirements and cover the necessary advances in solar cell, blanket integration, and large solar array structures that are needed for this class of missions. Th is paper will summarize NASA's plans for high power SEP missions, initi al mission studies and power system requirements, plans for advanced photovoltaic technology development, and the status of specific cell and array technology development and testing that have already been conducted.

High-performance, flexible, deployable array development for space applications

NASA Technical Reports Server (NTRS)

Gehling, Russell N.; Armstrong, Joseph H.; Misra, Mohan S.

1994-01-01

Flexible, deployable arrays are an attractive alternative to conventional solar arrays for near-term and future space power applications, particularly due to their potential for high specific power and low storage volume. Combined with low-cost flexible thin-film photovoltaics, these arrays have the potential to become an enabling or an enhancing technology for many missions. In order to expedite the acceptance of thin-film photovoltaics for space applications, however, parallel development of flexible photovoltaics and the corresponding deployable structure is essential. Many innovative technologies must be incorporated in these arrays to ensure a significant performance increase over conventional technologies. For example, innovative mechanisms which employ shape memory alloys for storage latches, deployment mechanisms, and array positioning gimbals can be incorporated into flexible array design with significant improvement in the areas of cost, weight, and reliability. This paper discusses recent activities at Martin Marietta regarding the development of flexible, deployable solar array technology. Particular emphasis is placed on the novel use of shape memory alloys for lightweight deployment elements to improve the overall specific power of the array. Array performance projections with flexible thin-film copper-indium-diselenide (CIS) are presented, and government-sponsored solar array programs recently initiated at Martin Marietta through NASA and Air Force Phillips Laboratory are discussed.

Electricity from photovoltaic solar cells. Flat-Plate Solar Array Project of the US Department of Energy's National Photovoltaics Program: 10 years of progress

NASA Technical Reports Server (NTRS)

Christensen, Elmer

1985-01-01

The objectives were to develop the flat-plate photovoltaic (PV) array technologies required for large-scale terrestrial use late in the 1980s and in the 1990s; advance crystalline silicon PV technologies; develop the technologies required to convert thin-film PV research results into viable module and array technology; and to stimulate transfer of knowledge of advanced PV materials, solar cells, modules, and arrays to the PV community. Progress reached on attaining these goals, along with future recommendations are discussed.

Photovoltaic solar array technology required for three wide scale generating systems for terrestrial applications: rooftop, solar farm, and satellite

NASA Technical Reports Server (NTRS)

Berman, P. A.

1972-01-01

Three major options for wide-scale generation of photovoltaic energy for terrestrial use are considered: (1) rooftop array, (2) solar farm, and (3) satellite station. The rooftop array would use solar cell arrays on the roofs of residential or commercial buildings; the solar farm would consist of large ground-based arrays, probably in arid areas with high insolation; and the satellite station would consist of an orbiting solar array, many square kilometers in area. The technology advancement requirements necessary for each option are discussed, including cost reduction of solar cells and arrays, weight reduction, resistance to environmental factors, reliability, and fabrication capability, including the availability of raw materials. The majority of the technology advancement requirements are applicable to all three options, making possible a flexible basic approach regardless of the options that may eventually be chosen. No conclusions are drawn as to which option is most advantageous, since the feasibility of each option depends on the success achieved in the technology advancement requirements specified.

Shaping of parabolic cylindrical membrane reflectors for the Dart Precision Test Bed

NASA Technical Reports Server (NTRS)

Morgan, R.; Agnes, Gregory S.; Dragovan, M.; Barber, D.; Marcin, M.; White, C.; Dooley, J.; Hatheway, A.

2004-01-01

The DART is a new telescope architecture consisting of a single aperture formed from two cylindrical parabolic reflectors. The system is ideally suited to using tensioned membranes for the reflective surfaces, owing to the zero Gaussian curvature of a cylindrical parabola. In this paper, we present experimental measurements for shaping the membranes by using curved boundary elements to achieve coarse shaping, and a pair of precision rails shaped by moments and forces at the ends, and lightly pushed into the surface, to provide fine shape control.

The Engineering Development Array: A Low Frequency Radio Telescope Utilising SKA Precursor Technology

NASA Astrophysics Data System (ADS)

Wayth, Randall; Sokolowski, Marcin; Booler, Tom; Crosse, Brian; Emrich, David; Grootjans, Robert; Hall, Peter J.; Horsley, Luke; Juswardy, Budi; Kenney, David; Steele, Kim; Sutinjo, Adrian; Tingay, Steven J.; Ung, Daniel; Walker, Mia; Williams, Andrew; Beardsley, A.; Franzen, T. M. O.; Johnston-Hollitt, M.; Kaplan, D. L.; Morales, M. F.; Pallot, D.; Trott, C. M.; Wu, C.

2017-08-01

We describe the design and performance of the Engineering Development Array, which is a low-frequency radio telescope comprising 256 dual-polarisation dipole antennas working as a phased array. The Engineering Development Array was conceived of, developed, and deployed in just 18 months via re-use of Square Kilometre Array precursor technology and expertise, specifically from the Murchison Widefield Array radio telescope. Using drift scans and a model for the sky brightness temperature at low frequencies, we have derived the Engineering Development Array's receiver temperature as a function of frequency. The Engineering Development Array is shown to be sky-noise limited over most of the frequency range measured between 60 and 240 MHz. By using the Engineering Development Array in interferometric mode with the Murchison Widefield Array, we used calibrated visibilities to measure the absolute sensitivity of the array. The measured array sensitivity matches very well with a model based on the array layout and measured receiver temperature. The results demonstrate the practicality and feasibility of using Murchison Widefield Array-style precursor technology for Square Kilometre Array-scale stations. The modular architecture of the Engineering Development Array allows upgrades to the array to be rolled out in a staged approach. Future improvements to the Engineering Development Array include replacing the second stage beamformer with a fully digital system, and to transition to using RF-over-fibre for the signal output from first stage beamformers.

Recent results from advanced research on space solar cells at NASA

NASA Technical Reports Server (NTRS)

Flood, Dennis J.

1990-01-01

The NASA program in space photovoltaic research and development encompasses a wide range of emerging options for future space power systems, and includes both cell and array technology development. The long range goals are to develop technology capable of achieving 300 W/kg for planar arrays, and 300 W/sq m for concentrator arrays. InP and GaAs planar and concentrator cell technologies are under investigation for their potential high efficiency and good radiation resistance. The Advanced Photovoltaic Solar Array (APSA) program is a near term effort aimed at demonstrating 130 W/kg beginning of life specific power using thin (62 pm) silicon cells. It is intended to be technology transparent to future high efficiency cells and provides the baseline for development of the 300 W/kg array.

Structural Coloration of a Colloidal Amorphous Array is Intensified by Carbon Nanolayers.

PubMed

Takeoka, Yukikazu; Iwata, Masanori; Seki, Takahiro; Nueangnoraj, Khanin; Nishihara, Hirotomo; Yoshioka, Shinya

2018-04-10

In this study, we introduce the possibility of applying a colloidal amorphous array composed of fine silica particles as a structural-color material to invisible information technology. The appearance of a thick filmlike colloidal amorphous array formed from fine silica particles is considerably influenced by incoherent light scattering across the entire visible region. Therefore, regardless of the diameter of the fine silica particles, the thick colloidal amorphous array exhibits a white color to the naked eye. When carbon is uniformly deposited in the colloidal amorphous array by a pressure-pulsed chemical vapor deposition method, incoherent light scattering in the colloidal amorphous array is suppressed. As a result, coherent light scattering due to the short-range order in the colloidal amorphous array becomes conspicuous and the array exhibits a vivid structural color. As structures, such as letters and pictures, can be drawn using this technology, the colloidal amorphous array as a structural-colored material may also be applicable for invisible information technology.

Space Photovoltaic Research and Technology 1986. High Efficiency, Space Environment, and Array Technology

NASA Technical Reports Server (NTRS)

1987-01-01

The conference provided a forum to assess the progress made, the problems remaining, and the strategy for the future of photovoltaic research. Cell research and technology, space environmental effects, array technology and applications were discussed.

Effects of Relaxing and Arousing Music during Imagery Training on Dart-Throwing Performance, Physiological Arousal Indices, and Competitive State Anxiety

PubMed Central

Kuan, Garry; Morris, Tony; Kueh, Yee Cheng; Terry, Peter C.

2018-01-01

Music that is carefully selected to match the requirements of activities and the characteristics of individuals has been shown to produce significant impacts on performance enhancement (Priest et al., 2004). There is also evidence that music can enhance imagery (Grocke and Wigram, 2007), although few studies have investigated the effects of music on imagery in the context of sport skills. In the present study, the effects of relaxing and arousing music during imagery on dart-throwing performance, physiological arousal indices, and competitive state anxiety, were investigated among 63 novice dart throwers. Participants had moderate-to-high imagery ability and were randomly assigned to unfamiliar relaxing music (URM), unfamiliar arousing music (UAM), or no music (NM) groups. Performance was assessed by 40 dart throws at a concentric circles dartboard before and after 12 imagery sessions over 4 weeks. Measures of galvanic skin response (GSR), peripheral temperature (PT), and heart rate (HR) were taken during imagery sessions 1 and 12, and the Competitive State Anxiety Inventory-2 Revised (CSAI-2R) was administered prior to the pre- and post-intervention performance task. Dart-throwing gain scores were significantly higher for URM than for UAM and NM, with no significant difference between UAM and NM (URM = 37.24 ± 5.66, UAM = 17.57 ± 5.30, and NM = 13.19 ± 6.14, F2,62 = 5.03, p = 0.01, η2 = 0.14). GSR, PT, and HR reflected lower arousal for URM than for UAM or NM. Significant decreases in somatic anxiety were evident for URM and UAM but not NM. Significant decreases in cognitive anxiety were evident for URM and NM but not UAM. Significant increases in self-confidence were evident for URM but not UAM or NM. Performance improved in all three conditions but URM was associated with the largest performance gain, the lowest physiological indices of arousal, and the most positive CSAI-2R profiles. Listening to relaxing music during imagery may have benefits for performance in other fine motor skills. PMID:29459837

Drug-Associated Risk Tool: development and validation of a self-assessment questionnaire to screen for hospitalised patients at risk for drug-related problems.

PubMed

Kaufmann, Carole P; Stämpfli, Dominik; Mory, Nadine; Hersberger, Kurt E; Lampert, Markus L

2018-03-09

Identifying patients with a high risk for drug-related problems (DRPs) might optimise the allocation of targeted pharmaceutical care during the hospital stay and on discharge. To develop a self-assessment screening tool to identify patients at risk for DRPs and validate the tool regarding feasibility, acceptability and the reliability of the patients' answers. Prospective validation study. Two mid-sized hospitals (300-400 beds). 195 patients, exclusion criteria: under 18 years old, patients with a health status not allowing a meaningful communication (eg, delirium, acute psychosis, advanced dementia, aphasia, clouded consciousness state), palliative or terminally ill patients. Twenty-seven risk factors for the development of DRPs, identified in a previous study, provided the basis of the self-assessment questionnaire, the Drug-Associated Risk Tool (DART). Consenting patients filled in DART, and we compared their answers with objective patient data from medical records and laboratory data. One hundred and sixty-four patients filled in DART V.1.0 in an average time of 7 min. After a first validation, we identified statements with a low sensitivity and revised the wording of the questions related to heart insufficiency, renal impairment or liver impairment. The revised DART (V.2.0) was validated in 31 patients presenting heart insufficiency, renal impairment or liver impairment as comorbidity and reached an average specificity of 88% (range 27-100) and an average sensitivity of 67% (range 21-100). DART showed a satisfying feasibility and reliability. The specificity of the statements was mostly high. The sensitivity varied and was higher in statements concerning diseases that require regular disease control and attention to self-care and drug management. Asking patients about their conditions, medications and related problems can facilitate getting a first, broad picture of the risk for DRPs and possible pharmaceutical needs. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

The disposition of impact ejecta resulting from the AIDA-DART mission to binary asteroid 65803 Didymos: an independent investigation

NASA Astrophysics Data System (ADS)

Richardson, James E.; O'Brien, David P.

2016-10-01

If all goes as planned, in the year 2020 a joint ESA and NASA mission will be launched that will rendezvous with the near-Earth binary asteroid system 65803 Didymos in the fall of 2022. The European component, the Asteroid Impact & Deflection Assessment (AIDA) spacecraft will arrive first and characterize the system, which consists of a ~800 m diameter primary and a ~160 m diameter secondary, orbiting a common center of mass at a semi-major axis distance of ~1200 m with a orbital period of 11.9 hr. Following system characterization, the AIDA spacecraft will remove to a safe distance while the NASA component, the 300 kg Double Asteroid Redirection Test (DART) spacecraft collides with the trailing edge of the secondary body (with respect to the binary's retrograde mutual orbit). Meanwhile, the AIDA spacecraft will conduct observations of this impact and its aftermath, specifically looking for changes made to the primary, the secondary, and their mutual orbit as a result of the DART collision. Of particular interest is the ballistic flight and final disposition of the ejecta produced by the impact cratering process, not just from the standpoint of scientific study, but also from the standpoint of AIDA spacecraft safety.In this study, we investigate a series of hypothetical DART impacts utilizing a semi-empirical, numerical impact ejecta plume model originally developed for the Deep Impact mission and designed specifically with impacts on small bodies in mind. The resulting excavated mass is discretized into 7200 individual tracer particles, each representing a unique combination of speed, mass, and ejected direction. The trajectory of each tracer is computed numerically under the gravitational influence of both primary and secondary, along with the effects of solar radiation pressure. Each tracer is followed until it either impacts a body or escapes the system, whereupon tracking is continued in the heliocentric frame using an N-body integrator. Various impact scenarios will be explored, along with a number of ejecta particle sizes, with the aim of characterizing the most likely final ejecta dispositions resulting from the DART impact, and the safest vantages from which the AIDA spacecraft can observe this event.

Modeling momentum transfer by the DART spacecraft into the moon of Didymos

NASA Astrophysics Data System (ADS)

Stickle, Angela M.; Atchison, Justin A.; Barnouin, Olivier S.; Cheng, Andy F.; Ernst, Carolyn M.; Richardson, Derek C.; Rivkin, Andy S.

2015-11-01

The Asteroid Impact and Deflection Assessment (AIDA) mission is a joint concept between NASA and ESA designed to test the effectiveness of a kinetic impactor in deflecting an asteroid. The mission is composed of two independent, but mutually supportive, components: the NASA-led Double Asteroid Redirect Test (DART), and the ESA-led Asteroid Impact Monitoring (AIM) mission. The spacecraft will be sent to the near-Earth binary asteroid 65803 Didymos, which makes unusually close approaches to Earth in 2022 and 2024. These close approaches make it an ideal target for a kinetic impactor asteroid deflection demonstration, as it will be easily observable from Earth-based observatories. The ~2 m3, 300 kg DART spacecraft will impact the moon of the binary system at 6.25 km/s. The deflection of the moon will then be determined by the orbiting AIM spacecraft and from ground-based observations by measuring the change in the moon’s orbital period. A modeling study supporting this mission concept was performed to determine the expected momentum transfer to the moon following impact. The combination of CTH hydrocode models, analytical scaling predictions, and N-body pkdgrav simulations helps to constrain the expected results of the kinetic impactor experiment.To better understand the large parameter space (including material strength, porosity, impact location and angle), simulations of the DART impact were performed using the CTH hydrocode. The resultant crater size, velocity imparted to the moon, and momentum transfer were calculated for all cases. For “realistic” asteroid types, simulated DART impacts produce craters with diameters on the order of 10 m, an imparted Δv of 0.5-2 mm/s and a dimensionless momentum enhancement (“beta factor”) of 1.07-5 for targets ranging from a highly porous aggregate to a fully dense rock. These results generally agree with predictions from theoretical and analytical studies. Following impact, pkdgrav simulations of the system evolution track changes in the orbital period of the moon and examine the effects of the shapes of Didymos and its moon on the deflection. These simulations indicate that the shapes of the bodies can influence the subsequent dynamics of the moon.

Potential Use of Deep-ocean Bottom Temperatures Measured by NOAA's Operational DART Systems in Identifying Long-term Climate Trends

NASA Astrophysics Data System (ADS)

Eble, M. C.; Mungov, G.

2016-12-01

The National Oceanic and Atmospheric Administration holds more than 3200 months of ocean bottom temperature time series recorded by the network of Deep-ocean Assessment and Reporting of Tsunami (DART) developed for reporting bottom pressure in support of tsunami detection and warning. Measurements were made at more than 40 locations sited predominately within the Pacific Ocean, with a lesser number made at sites in the North Atlantic. Since 2003, time series data were collected at isolated locations and at those in close proximity to sites measuring other ocean and atmosphere parameters (e.g. TAO/TRITON), at depths ranging from approximately 1800 m to nearly 6000 m. A full 2300 months of temperature measurements were made by DART systems deployed at or below 4000 m. These time series offer the potential of narrowing a gap in the global ocean observing system (OceanObs09) by exposing long-term climate trends at more locations in the ocean deep then now available. The potential held by these data given specific limitations is the focus of this investigation. Limitations stem from the primary function of temperature being to correct pressure. The sole function of temperature has historically been to provide a correction to pressure so little attention has been paid to optimizing temperature measurements for long-term investigations. Temperature counts, like those of pressure, are converted to engineering units using calibration coefficients supplied by the manufacturer but temperatures are only coarsely calibrated. In addition, the response of each pressure transducer to ocean pressure varies by deployment and location. Time series of DART temperature frequency counts recorded during specific single deployments were processed and analyzed to identify consistent trends and explore methodologies that could be adopted in order to improve the utility (NOAA, 2014) of DART temperature measurements for climate studies. These data represent a vast amount of untapped Environmental Intelligence that have the potential to contribute to an understanding of heat storage within the oceans and the possible prediction of earth's response.

The Asteroid Impact and Deflection Assessment (AIDA) mission: Science Proximity Operations

NASA Astrophysics Data System (ADS)

Barnouin, Olivier; Bellerose, Julie; Carnelli, Ian; Carrol, Kieran; Ciarletti, Valérie; Cheng, Andrew F.; Galvez, Andres; Green, Simon F.; Grieger, Bjorn; Hirabayashi, Masatoshi; Herique, Alain; Kueppers, Michael; Minton, David A.; Mellab, Karim; Michel, Patrick; Rivkin, Andrew S.; Rosenblatt, Pascal; Tortora, Paolo; Ulamec, Stephan; Vincent, Jean-Baptiste; Zannoni, Marco

2016-10-01

The moon of the near-Earth binary asteroid 65803 Didymos is the target of the Asteroid Impact and Deflection Assessment (AIDA) mission. This mission is a joint effort between NASA and ESA to investigate the effectiveness of a kinetic impactor in deflecting an asteroid. The mission is composed of two components: the NASA-led Double Asteroid Redirect Test (DART) that will impact Didymos' moon (henceforth Didymos B), and the ESA-led Asteroid Impact Mission (AIM) that will survey the Didymos system. Both will undertake proximity operations to characterize the physical and dynamical properties of the Didymos system that are of maximum importance in the joint AIDA mission to understand the factors at play when assessing the mometum transfer that follows DART's impact into Didymos B. Using much of ESA's Rosetta experience, the AIM mission will undertake proximity operations both before and after DART's impact. AIM's chracterization includes measuring the precise orbital configuration, masses, internal properties, surface geology and regolith properties of the primary and secondary, using visible and thermal imaging, radar measurements and radio science data. AIM will also release the small MASCOT-2 lander, as well as a suite of a CubeSats to help achieve these objectives. DART proximity observations include two phases of imaging. The first makes use of a suite of long range images that will add light curve data to what will be collected from Earth. These data will refine the orbit period of Didymos B, and provide constraints for modeling the shape of both Didymos A and B. The second phase begins just under an hour before impact when resolved imaging of the Didymos system provides further shape model constraints for the visble parts of both Didymos A and B, some possible constraints on the mass of Didymos B and key geological information of both objects and the impact site. In this presentation, we will summarize the proximity operations undertaken by both DART and AIM needed to achieve the scientific objectives of the AIDA mission using a broad suite of scientific experiments.

Dynamic Acquisition and Retrieval Tool (DART) for Comet Sample Return : Session: 2.06.Robotic Mobility and Sample Acquisition Systems

NASA Technical Reports Server (NTRS)

Badescu, Mircea; Bonitz, Robert; Kulczycki, Erick; Aisen, Norman; Dandino, Charles M.; Cantrell, Brett S.; Gallagher, William; Shevin, Jesse; Ganino, Anthony; Haddad, Nicolas;

Development of A Tsunami Magnitude Scale Based on DART Buoy Data

NASA Astrophysics Data System (ADS)

Leiva, J.; Polet, J.

2016-12-01

The quantification of tsunami energy has evolved through time, with a number of magnitude and intensity scales employed in the past century. Most of these scales rely on coastal measurements, which may be affected by complexities due to near-shore bathymetric effects and coastal geometries. Moreover, these datasets are generated by tsunami inundation, and thus cannot serve as a means of assessing potential tsunami impact prior to coastal arrival. With the introduction of a network of ocean buoys provided through the Deep-ocean Assessment and Reporting of Tsunamis (DART) project, a dataset has become available that can be exploited to further our current understanding of tsunamis and the earthquakes that excite them. The DART network consists of 39 stations that have produced estimates of sea-surface height as a function of time since 2003, and are able to detect deep ocean tsunami waves. Data collected at these buoys for the past decade reveals that at least nine major tsunami events, such as the 2011 Tohoku and 2013 Solomon Islands events, produced substantial wave amplitudes across a large distance range that can be implemented in a DART data based tsunami magnitude scale. We present preliminary results from the development of a tsunami magnitude scale that follows the methods used in the development of the local magnitude scale by Charles Richter. Analogous to the use of seismic ground motion amplitudes in the calculation of local magnitude, maximum ocean height displacements due to the passage of tsunami waves will be related to distance from the source in a least-squares exponential regression analysis. The regression produces attenuation curves based on the DART data, a site correction term, attenuation parameters, and an amplification factor. Initially, single event based regressions are used to constrain the attenuation parameters. Additional iterations use the parameters of these event-based fits as a starting point to obtain a stable solution, and include the calculation of station corrections, in order to obtain a final amplification factor for each event, which is used to calculate its tsunami magnitude.

Effects of Relaxing and Arousing Music during Imagery Training on Dart-Throwing Performance, Physiological Arousal Indices, and Competitive State Anxiety.

PubMed

Kuan, Garry; Morris, Tony; Kueh, Yee Cheng; Terry, Peter C

2018-01-01

Music that is carefully selected to match the requirements of activities and the characteristics of individuals has been shown to produce significant impacts on performance enhancement (Priest et al., 2004). There is also evidence that music can enhance imagery (Grocke and Wigram, 2007), although few studies have investigated the effects of music on imagery in the context of sport skills. In the present study, the effects of relaxing and arousing music during imagery on dart-throwing performance, physiological arousal indices, and competitive state anxiety, were investigated among 63 novice dart throwers. Participants had moderate-to-high imagery ability and were randomly assigned to unfamiliar relaxing music (URM), unfamiliar arousing music (UAM), or no music (NM) groups. Performance was assessed by 40 dart throws at a concentric circles dartboard before and after 12 imagery sessions over 4 weeks. Measures of galvanic skin response (GSR), peripheral temperature (PT), and heart rate (HR) were taken during imagery sessions 1 and 12, and the Competitive State Anxiety Inventory-2 Revised (CSAI-2R) was administered prior to the pre- and post-intervention performance task. Dart-throwing gain scores were significantly higher for URM than for UAM and NM, with no significant difference between UAM and NM (URM = 37.24 ± 5.66, UAM = 17.57 ± 5.30, and NM = 13.19 ± 6.14, F 2,62 = 5.03, p = 0.01, η 2 = 0.14). GSR, PT, and HR reflected lower arousal for URM than for UAM or NM. Significant decreases in somatic anxiety were evident for URM and UAM but not NM. Significant decreases in cognitive anxiety were evident for URM and NM but not UAM. Significant increases in self-confidence were evident for URM but not UAM or NM. Performance improved in all three conditions but URM was associated with the largest performance gain, the lowest physiological indices of arousal, and the most positive CSAI-2R profiles. Listening to relaxing music during imagery may have benefits for performance in other fine motor skills.

NASA photovoltaic research and technology

NASA Technical Reports Server (NTRS)

Flood, Dennis J.

1988-01-01

NASA photovoltaic R and D efforts address future Agency space mission needs through a comprehensive, integrated program. Activities range from fundamental studies of materials and devices to technology demonstrations of prototype hardware. The program aims to develop and apply an improved understanding of photovoltaic energy conversion devices and systems that will increase the performance, reduce the mass, and extend the lifetime of photovoltaic arrays for use in space. To that end, there are efforts aimed at improving cell efficiency, reducing the effects of space particulate radiation damage (primarily electrons and protons), developing ultralightweight cells, and developing advanced ray component technology for high efficiency concentrator arrays and high performance, ultralightweight arrays. Current goals that have been quantified for the program are to develop cell and array technology capable of achieving 300 watts/kg for future missions for which mass is a critical factor, or 300 watts/sq m for future missions for which array size is a major driver (i.e., Space Station). A third important goal is to develop cell and array technology which will survive the GEO space radiation environment for at least 10 years.

Cities, traffic, and CO2: A multidecadal assessment of trends, drivers, and scaling relationships

PubMed Central

Gately, Conor K.; Hutyra, Lucy R.; Sue Wing, Ian

2015-01-01

Emissions of CO2 from road vehicles were 1.57 billion metric tons in 2012, accounting for 28% of US fossil fuel CO2 emissions, but the spatial distributions of these emissions are highly uncertain. We develop a new emissions inventory, the Database of Road Transportation Emissions (DARTE), which estimates CO2 emitted by US road transport at a resolution of 1 km annually for 1980–2012. DARTE reveals that urban areas are responsible for 80% of on-road emissions growth since 1980 and for 63% of total 2012 emissions. We observe nonlinearities between CO2 emissions and population density at broad spatial/temporal scales, with total on-road CO2 increasing nonlinearly with population density, rapidly up to 1,650 persons per square kilometer and slowly thereafter. Per capita emissions decline as density rises, but at markedly varying rates depending on existing densities. We make use of DARTE’s bottom-up construction to highlight the biases associated with the common practice of using population as a linear proxy for disaggregating national- or state-scale emissions. Comparing DARTE with existing downscaled inventories, we find biases of 100% or more in the spatial distribution of urban and rural emissions, largely driven by mismatches between inventory downscaling proxies and the actual spatial patterns of vehicle activity at urban scales. Given cities’ dual importance as sources of CO2 and an emerging nexus of climate mitigation initiatives, high-resolution estimates such as DARTE are critical both for accurately quantifying surface carbon fluxes and for verifying the effectiveness of emissions mitigation efforts at urban scales. PMID:25847992

The 2004 Sumatra tsunami in the Southeastern Pacific Ocean: New Global Insight from Observations and Modeling

NASA Astrophysics Data System (ADS)

Rabinovich, A. B.; Titov, V. V.; Moore, C. W.; Eblé, M. C.

2017-10-01

The 2004 Sumatra tsunami was an unprecedented global disaster measured throughout the world oceans. The present study focused on a region of the southeastern Pacific Ocean where the "westward" circumferentially propagating tsunami branch converged with the "eastward" branch, based on data from fortuitously placed Chilean DART 32401 and tide gauges along the coast of South America. By comparison of the tsunami and background spectra, we suppressed the influence of topography and reconstructed coastal "spectral ratios" that were in close agreement with a ratio at DART 32401 and spectral ratios in other oceans. Findings indicate that even remote tsunami records carry spectral source signatures ("birth-marks"). The 2004 tsunami waves were found to occupy the broad frequency band of 0.25-10 cph with the prominent ratio peak at period of 40 min related to the southern fast-slip source domain. This rupture "hot-spot" of ˜350 km was responsible for the global impact of the 2004 tsunami. Data from DART 32401 provided validation of model results: the simulated maximum tsunami wave height of 2.25 cm was a conservative approximation to the measured height of 2.05 cm; the computed tsunami travel time of 25 h 35 min to DART 32401, although 20 min earlier than the actual travel time, provided a favorable result in comparison with 24 h 25 min estimated from classical kinematic theory. The numerical simulations consistently reproduced the wave height changes observed along the coast of South America, including local amplification of tsunami waves at the northern stations of Arica (72 cm) and Callao (67 cm).

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ikonomou, M.G.; Crewe, N.F.; Fischer, M.

It has been demonstrated that marine mammals accumulate high concentrations of lipophilic organochlorine contaminants in blubber. As predators of the high trophic level they have also been used to evaluate contamination in the marine environment. Sampling of living marine mammals using a microsample (100 to 200 mg) biopsy dart technique offers a potentially invaluable chronicle in assessing levels and types of persistent environmental pollutants from a sample in which age, sex and other genetic information can additionally be ascertained. The authors have explored analytical methodology based on a high sensitivity detection system (HRGC/HRMS) which provides multi-residue determinations from biopsy dartmore » microsamples. Lipid content and the concentrations of PCDDs, PCDFs and non-ortho and mono-ortho substituted PCBs were measured in 100 mg biopsy dart replicates taken from a killer whale carcass and in three strata of the blubber of that carcass. Statistically acceptable results were obtained from the dart replicates which compared very well with those of the blubber strata. Analytical data from 100 mg extractions from an established in house blubber CRM also compared well against a series of 2.5 g extractions of that CRM. The extraction and cleanup procedures used also allow for the determination of other organohalogen contaminants such as DDT and other pesticides, all the remaining PCBs, polychlorinated diphenylethers and brominated residues. The strengths and limitations of the analytical methodology and the biopsy dart as a sampling tool and pollution predicator will be illustrated in terms of method accuracy and precision, glassware and procedural blanks associated with each extraction batch, and the incorporation of an in house micro reference standard.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Jared A.; Hacker, Joshua P.; Monache, Luca Delle

A current barrier to greater deployment of offshore wind turbines is the poor quality of numerical weather prediction model wind and turbulence forecasts over open ocean. The bulk of development for atmospheric boundary layer (ABL) parameterization schemes has focused on land, partly due to a scarcity of observations over ocean. The 100-m FINO1 tower in the North Sea is one of the few sources worldwide of atmospheric profile observations from the sea surface to turbine hub height. These observations are crucial to developing a better understanding and modeling of physical processes in the marine ABL. In this paper we usemore » the WRF single column model (SCM), coupled with an ensemble Kalman filter from the Data Assimilation Research Testbed (DART), to create 100-member ensembles at the FINO1 location. The goal of this study is to determine the extent to which model parameter estimation can improve offshore wind forecasts. Combining two datasets that provide lateral forcing for the SCM and two methods for determining z 0, the time-varying sea-surface roughness length, we conduct four WRF-SCM/DART experiments over the October-December 2006 period. The two methods for determining z 0 are the default Fairall-adjusted Charnock formulation in WRF, and using parameter estimation techniques to estimate z 0 in DART. Using DART to estimate z 0 is found to reduce 1-h forecast errors of wind speed over the Charnock-Fairall z 0 ensembles by 4%–22%. Finally, however, parameter estimation of z 0 does not simultaneously reduce turbulent flux forecast errors, indicating limitations of this approach and the need for new marine ABL parameterizations.« less