KSC-2009-6402

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - A post-launch news conference is held in the NASA Press Site auditorium at NASA's Kennedy Space Center in Florida after the successful launch of space shuttle Atlantis. From left are Public Affairs moderator Mike Curie; Bill Gerstenmaier, associate administrator for Space Operations; Mike Moses, chair, Mission Management Team; and Mike Leinbach, space shuttle launch director. Liftoff of Atlantis on its STS-129 mission came at 2:28 p.m. EST Nov. 16 from Launch Pad 39A. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

A study with ESI PAM-STAMP® on the influence of tool deformation on final part quality during a forming process

NASA Astrophysics Data System (ADS)

Vrolijk, Mark; Ogawa, Takayuki; Camanho, Arthur; Biasutti, Manfredi; Lorenz, David

2018-05-01

As a result from the ever increasing demand to produce lighter vehicles, more and more advanced high-strength materials are used in automotive industry. Focusing on sheet metal cold forming processes, these materials require high pressing forces and exhibit large springback after forming. Due to the high pressing forces deformations occur in the tooling geometry, introducing dimensional inaccuracies in the blank and potentially impact the final springback behavior. As a result the tool deformations can have an impact on the final assembly or introduce cosmetic defects. Often several iterations are required in try-out to obtain the required tolerances, with costs going up to as much as 30% of the entire product development cost. To investigate the sheet metal part feasibility and quality, in automotive industry CAE tools are widely used. However, in current practice the influence of the tool deformations on the final part quality is generally neglected and simulations are carried out with rigid tools to avoid drastically increased calculation times. If the tool deformation is analyzed through simulation it is normally done at the end of the drawing prosses, when contact conditions are mapped on the die structure and a static analysis is performed to check the deflections of the tool. But this method does not predict the influence of these deflections on the final quality of the part. In order to take tool deformations into account during drawing simulations, ESI has developed the ability to couple solvers efficiently in a way the tool deformations can be real-time included in the drawing simulation without high increase in simulation time compared to simulations with rigid tools. In this paper a study will be presented which demonstrates the effect of tool deformations on the final part quality.

5 CFR 532.279 - Special wage schedules for printing positions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Opaquer 4 Offset Press Helper 5 Bindery Machine Operator (Helper) 5 Film Assembler-Stripper (Single Flat-Single Color) 5 Platemaker (Single Color) 5 Film Assembler-Stripper (Partial and Composite Flats) 7... Cutter) 8 Bindery Machine Operator (Power Folder) 8 Film Assembler-Stripper (Multiple Flat-Multiple Color...

5 CFR 532.279 - Special wage schedules for printing positions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Opaquer 4 Offset Press Helper 5 Bindery Machine Operator (Helper) 5 Film Assembler-Stripper (Single Flat-Single Color) 5 Platemaker (Single Color) 5 Film Assembler-Stripper (Partial and Composite Flats) 7... Cutter) 8 Bindery Machine Operator (Power Folder) 8 Film Assembler-Stripper (Multiple Flat-Multiple Color...

KSC-2009-6403

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Media representatives and Twitter followers participate in a post-launch news conference in the NASA Press Site auditorium at NASA's Kennedy Space Center in Florida after the successful launch of space shuttle Atlantis. On the dais, from left, are Public Affairs moderator Mike Curie; Bill Gerstenmaier, associate administrator for Space Operations; Mike Moses, chair, Mission Management Team; and Mike Leinbach, space shuttle launch director. Liftoff of Atlantis on its STS-129 mission came at 2:28 p.m. EST Nov. 16 from Launch Pad 39A. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kim Shiflett

Main roll for an air press of a papermaking machine

DOEpatents

Beck, David A.

2004-03-09

A roll for use in an air press assembly of a papermaking machine has a pair of ends associated therewith. The roll includes a pair of edge portions with each edge portion extending to one of the pair of ends. Each edge portion has an edge surface portion composed of a first material, the first material having a first hardness. The roll further includes a middle portion located between the pair of edge portions, the middle portion having a middle surface portion composed of a second material. The second material has a second hardness, the second material being harder than the first material. The first material is preferably a soft, seal material which promotes reduced air leakage from the air press assembly.

Whole genome sequencing of Chinese clearhead icefish, Protosalanx hyalocranius.

PubMed

Liu, Kai; Xu, Dongpo; Li, Jia; Bian, Chao; Duan, Jinrong; Zhou, Yanfeng; Zhang, Minying; You, Xinxin; You, Yang; Chen, Jieming; Yu, Hui; Xu, Gangchun; Fang, Di-An; Qiang, Jun; Jiang, Shulun; He, Jie; Xu, Junmin; Shi, Qiong; Zhang, Zhiyong; Xu, Pao

2017-04-01

Chinese clearhead icefish, Protosalanx hyalocranius , is a representative icefish species with economic importance and special appearance. Due to its great economic value in China, the fish was introduced into Lake Dianchi and several other lakes from the Lake Taihu half a century ago. Similar to the Sinocyclocheilus cavefish, the clearhead icefish has certain cavefish-like traits, such as transparent body and nearly scaleless skin. Here, we provide the whole genome sequence of this surface-dwelling fish and generated a draft genome assembly, aiming at exploring molecular mechanisms for the biological interests. A total of 252.1 Gb of raw reads were sequenced. Subsequently, a novel draft genome assembly was generated, with the scaffold N50 reaching 1.163 Mb. The genome completeness was estimated to be 98.39 % by using the CEGMA evaluation. Finally, we annotated 19 884 protein-coding genes and observed that repeat sequences account for 24.43 % of the genome assembly. We report the first draft genome of the Chinese clearhead icefish. The genome assembly will provide a solid foundation for further molecular breeding and germplasm resource protection in Chinese clearhead icefish, as well as other icefishes. It is also a valuable genetic resource for revealing the molecular mechanisms for the cavefish-like characters. © The Authors 2017. Published by Oxford University Press.

SvABA: genome-wide detection of structural variants and indels by local assembly.

PubMed

Wala, Jeremiah A; Bandopadhayay, Pratiti; Greenwald, Noah F; O'Rourke, Ryan; Sharpe, Ted; Stewart, Chip; Schumacher, Steve; Li, Yilong; Weischenfeldt, Joachim; Yao, Xiaotong; Nusbaum, Chad; Campbell, Peter; Getz, Gad; Meyerson, Matthew; Zhang, Cheng-Zhong; Imielinski, Marcin; Beroukhim, Rameen

2018-04-01

Structural variants (SVs), including small insertion and deletion variants (indels), are challenging to detect through standard alignment-based variant calling methods. Sequence assembly offers a powerful approach to identifying SVs, but is difficult to apply at scale genome-wide for SV detection due to its computational complexity and the difficulty of extracting SVs from assembly contigs. We describe SvABA, an efficient and accurate method for detecting SVs from short-read sequencing data using genome-wide local assembly with low memory and computing requirements. We evaluated SvABA's performance on the NA12878 human genome and in simulated and real cancer genomes. SvABA demonstrates superior sensitivity and specificity across a large spectrum of SVs and substantially improves detection performance for variants in the 20-300 bp range, compared with existing methods. SvABA also identifies complex somatic rearrangements with chains of short (<1000 bp) templated-sequence insertions copied from distant genomic regions. We applied SvABA to 344 cancer genomes from 11 cancer types and found that short templated-sequence insertions occur in ∼4% of all somatic rearrangements. Finally, we demonstrate that SvABA can identify sites of viral integration and cancer driver alterations containing medium-sized (50-300 bp) SVs. © 2018 Wala et al.; Published by Cold Spring Harbor Laboratory Press.

Crack-Free, Nondistorting Can For Hot Isostatic Pressing

NASA Technical Reports Server (NTRS)

Juhas, John J.

1991-01-01

New method of canning specimens made of composites of arc-sprayed and plasma-sprayed tape reduces outgassing and warping during hot isostatic pressing. Produces can having reliable, crack-free seal and thereby helps to ensure pressed product of high quality. Specimen placed in ring of refractory metal between two face sheets, also of refractory metal. Assembly placed in die in vacuum hot press, where simultaneously heated and pressed until plates become diffusion-welded to ring, forming sealed can around specimen. Specimen becomes partially densified, and fits snugly within can. Ready for further densification by hot isostatic pressing.

Hong Kong: Ten Years After the Handover

DTIC Science & Technology

2007-06-29

Liberties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Freedom of Speech and Assembly...surrounding the proposed anti-sedition legislation in 2003. The freedoms of speech and assembly appear to have been largely respected by the Chinese and...undermined the civil liberties of Hong Kong residents. However, there have been perceived threats of the freedom of speech and assembly, and erosion of press

49. Neg. No. 7268C, ca. 1930, PhotographerUnknown, OVERALL VIEW OF ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

49. Neg. No. 7268C, ca. 1930, Photographer-Unknown, OVERALL VIEW OF THE FORD MOTOR COMPANY ASSEMBLY PLANT FROM THE HENRY FORD BRIDGE, PRIOR TO CONSTRUCTION OF THE PRESSED STEEL BUILDING - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Uzbekistan: Recent Developments and U.S. Interests

DTIC Science & Technology

2010-08-12

problems included arbitrary arrest and detention; denial of due process and fair trial; restrictions on freedom of speech , press, assembly, and...prevent their activities and dissuade them from meeting with foreigners. The government generally did not respect freedom of speech and the press. Slander

74. Neg. No. F116A, May 22, 1931, INTERIORPRESSED STEEL BUILDING, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

74. Neg. No. F-116A, May 22, 1931, INTERIOR-PRESSED STEEL BUILDING, SHOWING FENDER CRATE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Amorphous Metals and Composites as Mirrors and Mirror Assemblies

NASA Technical Reports Server (NTRS)

Hofmann, Douglas C. (Inventor); Davis, Gregory L. (Inventor); Agnes, Gregory S. (Inventor); Shapiro, Andrew A. (Inventor)

2016-01-01

A mirror or mirror assembly fabricated by molding, pressing, assembling, or depositing one or more bulk metal glass (BMG), bulk metal glass composite (BMGMC), or amorphous metal (AM) parts and where the optical surface and backing of the mirror can be fabricated without machining or polishing by utilizing the unique molding capabilities of this class of materials.

The structure and intermolecular forces of DNA condensates.

PubMed

Yoo, Jejoong; Aksimentiev, Aleksei

2016-03-18

Spontaneous assembly of DNA molecules into compact structures is ubiquitous in biological systems. Experiment has shown that polycations can turn electrostatic self-repulsion of DNA into attraction, yet the physical mechanism of DNA condensation has remained elusive. Here, we report the results of atomistic molecular dynamics simulations that elucidated the microscopic structure of dense DNA assemblies and the physics of interactions that makes such assemblies possible. Reproducing the setup of the DNA condensation experiments, we measured the internal pressure of DNA arrays as a function of the DNA-DNA distance, showing a quantitative agreement between the results of our simulations and the experimental data. Analysis of the MD trajectories determined the DNA-DNA force in a DNA condensate to be pairwise, the DNA condensation to be driven by electrostatics of polycations and not hydration, and the concentration of bridging cations, not adsorbed cations, to determine the magnitude and the sign of the DNA-DNA force. Finally, our simulations quantitatively characterized the orientational correlations of DNA in DNA arrays as well as diffusive motion of DNA and cations. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

KSC-2011-5247

NASA Image and Video Library

2011-07-08

CAPE CANAVERAL, Fla. -- Media from around the globe gather on the grounds of the Press Site at NASA's Kennedy Space Center in Florida to photograph and cover the prelaunch activities and lift off of space shuttle Atlantis on its STS-135 mission to the International Space Station. Seen towering above is the massive Vehicle Assembly Building. Satellite news trucks, trailers and automobiles can be seen in the parking lot with the massive Vehicle Assembly Building towering above. Atlantis began its final flight, with Commander Chris Ferguson, Pilot Doug Hurley and Mission Specialists Sandy Magnus and Rex Walheim on board, at 11:29 a.m. EDT July 8 to deliver the Raffaello multi-purpose logistics module packed with supplies and spare parts to the station. Also in Atlantis' payload bay is the Robotic Refueling Mission experiment that will investigate the potential for robotically refueling existing satellites in orbit. In addition, Atlantis will return with a failed ammonia pump module to help NASA better understand the failure mechanism and improve pump designs for future systems. STS-135 is the 33rd flight of Atlantis, the 37th shuttle mission to the space station, and the 135th and final mission of NASA's Space Shuttle Program. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Jim Grossmann

Genome puzzle master (GPM): an integrated pipeline for building and editing pseudomolecules from fragmented sequences.

PubMed

Zhang, Jianwei; Kudrna, Dave; Mu, Ting; Li, Weiming; Copetti, Dario; Yu, Yeisoo; Goicoechea, Jose Luis; Lei, Yang; Wing, Rod A

2016-10-15

Next generation sequencing technologies have revolutionized our ability to rapidly and affordably generate vast quantities of sequence data. Once generated, raw sequences are assembled into contigs or scaffolds. However, these assemblies are mostly fragmented and inaccurate at the whole genome scale, largely due to the inability to integrate additional informative datasets (e.g. physical, optical and genetic maps). To address this problem, we developed a semi-automated software tool-Genome Puzzle Master (GPM)-that enables the integration of additional genomic signposts to edit and build 'new-gen-assemblies' that result in high-quality 'annotation-ready' pseudomolecules. With GPM, loaded datasets can be connected to each other via their logical relationships which accomplishes tasks to 'group,' 'merge,' 'order and orient' sequences in a draft assembly. Manual editing can also be performed with a user-friendly graphical interface. Final pseudomolecules reflect a user's total data package and are available for long-term project management. GPM is a web-based pipeline and an important part of a Laboratory Information Management System (LIMS) which can be easily deployed on local servers for any genome research laboratory. The GPM (with LIMS) package is available at https://github.com/Jianwei-Zhang/LIMS CONTACTS: jzhang@mail.hzau.edu.cn or rwing@mail.arizona.eduSupplementary information: Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press.

Solid explosive plane-wave lenses pressed-to-shape with dies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Olinger, B.

2007-11-01

Solid-explosive plane-wave lenses 1", 2" and 4¼" in diameter have been mass-produced from components pressed-to-shape with aluminum dies. The method used to calculate the contour between the solid plane-wave lens components pressed-to-shape with the dies is explained. The steps taken to press, machine, and assemble the lenses are described. The method of testing the lenses, the results of those tests, and the corrections to the dies are reviewed. The work on the ½", 8", and 12" diameter lenses is also discussed.

40 CFR 1054.610 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2013 CFR

2013-07-01

... final assembly? 1054.610 Section 1054.610 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... EQUIPMENT Special Compliance Provisions § 1054.610 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly do not apply for handheld engines certified...

40 CFR 1054.610 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2012 CFR

2012-07-01

... final assembly? 1054.610 Section 1054.610 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... EQUIPMENT Special Compliance Provisions § 1054.610 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly do not apply for handheld engines certified...

40 CFR 1054.610 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2010 CFR

2010-07-01

... final assembly? 1054.610 Section 1054.610 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... EQUIPMENT Special Compliance Provisions § 1054.610 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly do not apply for handheld engines certified...

40 CFR 1054.610 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2011 CFR

2011-07-01

... final assembly? 1054.610 Section 1054.610 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... EQUIPMENT Special Compliance Provisions § 1054.610 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly do not apply for handheld engines certified...

40 CFR 1054.610 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2014 CFR

2014-07-01

... final assembly? 1054.610 Section 1054.610 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... EQUIPMENT Special Compliance Provisions § 1054.610 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly do not apply for handheld engines certified...

Translations on Eastern Europe, Political, Sociological, and Military Affairs, Number 1432

DTIC Science & Technology

1977-08-16

of thought by the individual, and the respective guarantees. Article 53 of the constitution states: "Citizens enjoy freedom of speech , the press...free expression of thought by the workers in our country through freedom of speech , press, assembly and public demonstration and organization is fully...unless the thinking of the masses is reflected. The thinking 6f the masses and its full expression through freedom of speech , the press and

Hot compression process for making edge seals for fuel cells

DOEpatents

Dunyak, Thomas J.; Granata, Jr., Samuel J.

1994-01-01

A hot compression process for forming integral edge seals in anode and cade assemblies wherein the assemblies are made to a nominal size larger than a finished size, beads of AFLAS are applied to a band adjacent the peripheral margins on both sides of the assemblies, the assemblies are placed in a hot press and compressed for about five minutes with a force sufficient to permeate the peripheral margins with the AFLAS, cooled and cut to finished size.

40 CFR 1048.612 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2012 CFR

2012-07-01

... final assembly? 1048.612 Section 1048.612 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Compliance Provisions § 1048.612 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly apply for engines certified under this part 1048, with the...

40 CFR 1048.612 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2010 CFR

2010-07-01

... final assembly? 1048.612 Section 1048.612 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Compliance Provisions § 1048.612 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly apply for engines certified under this part 1048, with the...

40 CFR 1048.612 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2013 CFR

2013-07-01

... final assembly? 1048.612 Section 1048.612 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Compliance Provisions § 1048.612 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly apply for engines certified under this part 1048, with the...

40 CFR 1048.612 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2011 CFR

2011-07-01

... final assembly? 1048.612 Section 1048.612 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Compliance Provisions § 1048.612 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly apply for engines certified under this part 1048, with the...

40 CFR 1048.612 - What is the exemption for delegated final assembly?

Code of Federal Regulations, 2014 CFR

2014-07-01

... final assembly? 1048.612 Section 1048.612 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Compliance Provisions § 1048.612 What is the exemption for delegated final assembly? The provisions of 40 CFR 1068.261 related to delegated final assembly apply for engines certified under this part 1048, with the...

57. Neg. No. F161D, Mar 18, 1933, INTERIORPRESSED STEEL AND ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

57. Neg. No. F-161D, Mar 18, 1933, INTERIOR-PRESSED STEEL AND WAREHOUSE BUILDINGS, 1ST FLOOR, SHOWING DAMAGE TO FREIGHT ELEVATOR SHAFT FROM EARTHQUAKE - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

48. Neg. No. P3120, ca. 1930, Photographer A. C. Gates, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

48. Neg. No. P-3120, ca. 1930, Photographer- A. C. Gates, Los Angeles, AERIAL VIEW OF THE FORD MOTOR COMPANY ASSEMBLY PLANT, PRIOR TO CONSTRUCTION OF THE PRESSED STEEL BUILDING, NOTE THE CLIPPER SHIPS IN THE BACKGROUND, AND THE OIL WELLS IN THE UPPER LEFT CORNER - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Draft genome of the lined seahorse, Hippocampus erectus.

PubMed

Lin, Qiang; Qiu, Ying; Gu, Ruobo; Xu, Meng; Li, Jia; Bian, Chao; Zhang, Huixian; Qin, Geng; Zhang, Yanhong; Luo, Wei; Chen, Jieming; You, Xinxin; Fan, Mingjun; Sun, Min; Xu, Pao; Venkatesh, Byrappa; Xu, Junming; Fu, Hongtuo; Shi, Qiong

2017-06-01

The lined seahorse, Hippocampus erectus , is an Atlantic species and mainly inhabits shallow sea beds or coral reefs. It has become very popular in China for its wide use in traditional Chinese medicine. In order to improve the aquaculture yield of this valuable fish species, we are trying to develop genomic resources for assistant selection in genetic breeding. Here, we provide whole genome sequencing, assembly, and gene annotation of the lined seahorse, which can enrich genome resource and further application for its molecular breeding. A total of 174.6 Gb (Gigabase) raw DNA sequences were generated by the Illumina Hiseq2500 platform. The final assembly of the lined seahorse genome is around 458 Mb, representing 94% of the estimated genome size (489 Mb by k-mer analysis). The contig N50 and scaffold N50 reached 14.57 kb and 1.97 Mb, respectively. Quality of the assembled genome was assessed by BUSCO with prediction of 85% of the known vertebrate genes and evaluated using the de novo assembled RNA-seq transcripts to prove a high mapping ratio (more than 99% transcripts could be mapped to the assembly). Using homology-based, de novo and transcriptome-based prediction methods, we predicted 20 788 protein-coding genes in the generated assembly, which is less than our previously reported gene number (23 458) of the tiger tail seahorse ( H. comes ). We report a draft genome of the lined seahorse. These generated genomic data are going to enrich genome resource of this economically important fish, and also provide insights into the genetic mechanisms of its iconic morphology and male pregnancy behavior. © The Authors 2017. Published by Oxford University Press.

Performance of Transuranic-Loaded Fully Ceramic Micro-Encapsulated Fuel in LWRs Final Report, Including Void Reactivity Evaluation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Michael A. Pope; R. Sonat Sen; Brian Boer

2011-09-01

The current focus of the Deep Burn Project is on once-through burning of transuranics (TRU) in light-water reactors (LWRs). The fuel form is called Fully-Ceramic Micro-encapsulated (FCM) fuel, a concept that borrows the tri-isotropic (TRISO) fuel particle design from high-temperature reactor technology. In the Deep Burn LWR (DB-LWR) concept, these fuel particles are pressed into compacts using SiC matrix material and loaded into fuel pins for use in conventional LWRs. The TRU loading comes from the spent fuel of a conventional LWR after 5 years of cooling. Unit cell and assembly calculations have been performed using the DRAGON-4 code tomore » assess the physics attributes of TRU-only FCM fuel in an LWR lattice. Depletion calculations assuming an infinite lattice condition were performed with calculations of various reactivity coefficients performed at each step. Unit cells and assemblies containing typical UO2 and mixed oxide (MOX) fuel were analyzed in the same way to provide a baseline against which to compare the TRU-only FCM fuel. Then, assembly calculations were performed evaluating the performance of heterogeneous arrangements of TRU-only FCM fuel pins along with UO2 pins.« less

A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana.

PubMed

Nowell, Reuben W; Elsworth, Ben; Oostra, Vicencio; Zwaan, Bas J; Wheat, Christopher W; Saastamoinen, Marjo; Saccheri, Ilik J; Van't Hof, Arjen E; Wasik, Bethany R; Connahs, Heidi; Aslam, Muhammad L; Kumar, Sujai; Challis, Richard J; Monteiro, Antónia; Brakefield, Paul M; Blaxter, Mark

2017-07-01

The mycalesine butterfly Bicyclus anynana, the "Squinting bush brown," is a model organism in the study of lepidopteran ecology, development, and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology; 128 Gb of raw Illumina data was filtered to 124 Gb and assembled to a final size of 475 Mb (∼×260 assembly coverage). Contigs were scaffolded using mate-pair, transcriptome, and PacBio data into 10 800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements and encodes a total of 22 642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes. We report a high-quality draft genome sequence for Bicyclus anynana. The genome assembly and annotated gene models are available at LepBase (http://ensembl.lepbase.org/index.html). © The Authors 2017. Published by Oxford University Press.

Method of making tantalum capacitors

DOEpatents

McMillan, April D.; Clausing, Robert E.; Vierow, William F.

1998-01-01

A method for manufacturing tantalum capacitors includes preparing a tantalum compact by cold pressing tantalum powder, placing the compact, along with loose refractory metal powder, in a microwave-transparent casket to form an assembly, and heating the assembly for a time sufficient to effect at least partial sintering of the compact and the product made by the method.

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site watch as space shuttle Atlantis springs into action from Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

The dual-function chaperone HycH improves assembly of the formate hydrogenlyase complex.

PubMed

Lindenstrauß, Ute; Skorupa, Philipp; McDowall, Jennifer S; Sargent, Frank; Pinske, Constanze

2017-08-11

The assembly of multi-protein complexes requires the concerted synthesis and maturation of its components and subsequently their co-ordinated interaction. The membrane-bound formate hydrogenlyase (FHL) complex is the primary hydrogen-producing enzyme in Escherichia coli and is composed of seven subunits mostly encoded within the hycA-I operon for [NiFe]-hydrogenase-3 (Hyd-3). The HycH protein is predicted to have an accessory function and is not part of the final structural FHL complex. In this work, a mutant strain devoid of HycH was characterised and found to have significantly reduced FHL activity due to the instability of the electron transfer subunits. HycH was shown to interact specifically with the unprocessed species of HycE, the catalytic hydrogenase subunit of the FHL complex, at different stages during the maturation and assembly of the complex. Variants of HycH were generated with the aim of identifying interacting residues and those that influence activity. The R70/71/K72, the Y79, the E81 and the Y128 variant exchanges interrupt the interaction with HycE without influencing the FHL activity. In contrast, FHL activity, but not the interaction with HycE, was negatively influenced by H37 exchanges with polar residues. Finally, a HycH Y30 variant was unstable. Surprisingly, an overlapping function between HycH with its homologous counterpart HyfJ from the operon encoding [NiFe]-hydrogenase-4 (Hyd-4) was identified and this is the first example of sharing maturation machinery components between Hyd-3 and Hyd-4 complexes. The data presented here show that HycH has a novel dual role as an assembly chaperone for a cytoplasmic [NiFe]-hydrogenase. © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

High pressure generation using scaled-up Kawai-cell

NASA Astrophysics Data System (ADS)

Shatskiy, A.; Katsura, T.; Litasov, K. D.; Shcherbakova, A. V.; Borzdov, Y. M.; Yamazaki, D.; Yoneda, A.; Ohtani, E.; Ito, E.

2011-11-01

A scaled-up version of a 6-8 Kawai-type multianvil apparatus equipped with 47-mm WC anvils has been developed at the Institute for the Study of the Earth's Interior for operation over pressure ranging up to 19 and 24 GPa using the conventional system with larger compressional volumes between 1.2 and 0.4 cm 3, respectively. This system is used under uniaxial compression along cube diagonal of the Kawai-cell up to the press load of 19 MN. Experiments are performed using octahedral pressure media (PM) made of MgO- and ZrO 2-based semi-sintered ceramics and unfired pyrophyllite gaskets. In this study we used "Toshiba-F" grade WC anvils allowing pressure generation up to 24 GPa. We perform pressure calibrations at room and high temperatures, with octahedron/anvil truncation edge-length ratios ( a0/ b, mm) of 12.2/6, 14/6, 14/7, 16/7, 18/7, 18/9, and 18/10. Different configurations show that an increase in edge-length ratio of a0/b permits the achievement of higher pressure, which agrees with the results of Frost at al. (Frost, D.J., Poe, B.T., Tronnes, R.G., Liebske, C., Duba, A., Rubie, D.C., 2004. A new large-volume multianvil system. Phys. Earth Planet. Inter. 143, 507). However, it also shifts the pressure maximum to higher press loads, in some cases exceeding the capacity of a press. Our and Frost et al. (2004) data reveal that the 14/6, 18/8, and 18/10 assemblies are the most suitable in generating pressures of up to 19-24 GPa at 19 MN press load limits. The assemblies with a low a0/ b ratio have a lower upper pressure limit; however, they exhibit a systematically higher efficiency in pressure generation at low press loads. Consequently, assemblages with high and low a0/ b ratios should be used in high and low pressure experiments, respectively. For example, the 18/12 assembly is suitable for 5-11 GPa pressure range (Stoyanov, E., Haussermann, U., Leinenweber, K., 2010. Large-volume multianvil cells designed for chemical synthesis at high pressures. High Pressure Res., 30, 175), whereas the 14/6, 18/8 ( Frost et al., 2004), and 18/10 assemblies are suitable for 22-24, 19-23, and 11-19 GPa pressure ranges, respectively. The maximum pressure generation achieved in the present study is 24 GPa, using the 14/6 assembly. This appears to be the maximum pressure level attainable by using WC anvils.

MAS NMR of HIV-1 protein assemblies

NASA Astrophysics Data System (ADS)

Suiter, Christopher L.; Quinn, Caitlin M.; Lu, Manman; Hou, Guangjin; Zhang, Huilan; Polenova, Tatyana

2015-04-01

The negative global impact of the AIDS pandemic is well known. In this perspective article, the utility of magic angle spinning (MAS) NMR spectroscopy to answer pressing questions related to the structure and dynamics of HIV-1 protein assemblies is examined. In recent years, MAS NMR has undergone major technological developments enabling studies of large viral assemblies. We discuss some of these evolving methods and technologies and provide a perspective on the current state of MAS NMR as applied to the investigations into structure and dynamics of HIV-1 assemblies of CA capsid protein and of Gag maturation intermediates.

Redundant Bearing Assembly

NASA Technical Reports Server (NTRS)

Wright, Jay M.

1995-01-01

Proposed redundant bearing assembly consists of two modified ball or roller bearings, one held by other. Outer race of inner bearing press-fit into inner race of outer bearing. Within each bearing, side walls of inner and outer races extended radially toward each other leaving only small gap. In assembly, one bearing continues to allow free rotation when other fails. Bearing wear monitored by examination of gaps between races. In alternative design, inner race of outer bearing and outer race of inner bearing manufactured as single piece.

Draft genome of the Northern snakehead, Channa argus.

PubMed

Xu, Jian; Bian, Chao; Chen, Kunci; Liu, Guiming; Jiang, Yanliang; Luo, Qing; You, Xinxin; Peng, Wenzhu; Li, Jia; Huang, Yu; Yi, Yunhai; Dong, Chuanju; Deng, Hua; Zhang, Songhao; Zhang, Hanyuan; Shi, Qiong; Xu, Peng

2017-04-01

The Northern snakehead (Channa argus), a member of the Channidae family of the Perciformes, is an economically important freshwater fish native to East Asia. In North America, it has become notorious as an intentionally released invasive species. Its ability to breathe air with gills and migrate short distances over land makes it a good model for bimodal breath research. Therefore, recent research has focused on the identification of relevant candidate genes. Here, we performed whole genome sequencing of C. argus to construct its draft genome, aiming to offer useful information for further functional studies and identification of target genes related to its unusual facultative air breathing. Findings: We assembled the C. argus genome with a total of 140.3 Gb of raw reads, which were sequenced using the Illumina HiSeq2000 platform. The final draft genome assembly was approximately 615.3 Mb, with a contig N50 of 81.4 kb and scaffold N50 of 4.5 Mb. The identified repeat sequences account for 18.9% of the whole genome. The 19 877 protein-coding genes were predicted from the genome assembly, with an average of 10.5 exons per gene. Conclusion: We generated a high-quality draft genome of C. argus, which will provide a valuable genetic resource for further biomedical investigations of this economically important teleost fish. © The Author 2017. Published by Oxford University Press.

rnaQUAST: a quality assessment tool for de novo transcriptome assemblies.

PubMed

Bushmanova, Elena; Antipov, Dmitry; Lapidus, Alla; Suvorov, Vladimir; Prjibelski, Andrey D

2016-07-15

Ability to generate large RNA-Seq datasets created a demand for both de novo and reference-based transcriptome assemblers. However, while many transcriptome assemblers are now available, there is still no unified quality assessment tool for RNA-Seq assemblies. We present rnaQUAST-a tool for evaluating RNA-Seq assembly quality and benchmarking transcriptome assemblers using reference genome and gene database. rnaQUAST calculates various metrics that demonstrate completeness and correctness levels of the assembled transcripts, and outputs them in a user-friendly report. rnaQUAST is implemented in Python and is freely available at http://bioinf.spbau.ru/en/rnaquast ap@bioinf.spbau.ru Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - With nearly 7 million pounds of thrust generated by twin solid rocket boosters and three main engines, space shuttle Atlantis zooms into the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kenny Allen

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site witness space shuttle Atlantis cut its way through the blue skies over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - With nearly 7 million pounds of thrust generated by twin solid rocket boosters and three main engines, space shuttle Atlantis races to orbit over Launch Pad 39A at NASA's Kennedy Space Center in Florida. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two ExPRESS Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Kenny Allen

Launch of Space Shuttle Atlantis / STS-129 Mission

NASA Image and Video Library

2009-11-16

CAPE CANAVERAL, Fla. - Twitter followers and media representatives at the NASA Press Site have front-row seats as space shuttle Atlantis launches through the clouds from Launch Pad 39A on a balmy Florida afternoon at NASA's Kennedy Space Center. Liftoff on its STS-129 mission came at 2:28 p.m. EST Nov. 16. Aboard are crew members Commander Charles O. Hobaugh; Pilot Barry E. Wilmore; and Mission Specialists Leland Melvin, Randy Bresnik, Mike Foreman and Robert L. Satcher Jr. On STS-129, the crew will deliver two Express Logistics Carriers to the International Space Station, the largest of the shuttle's cargo carriers, containing 15 spare pieces of equipment including two gyroscopes, two nitrogen tank assemblies, two pump modules, an ammonia tank assembly and a spare latching end effector for the station's robotic arm. Atlantis will return to Earth a station crew member, Nicole Stott, who has spent more than two months aboard the orbiting laboratory. STS-129 is slated to be the final space shuttle Expedition crew rotation flight. For information on the STS-129 mission and crew, visit http://www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts129/index.html. Photo credit: NASA/Gianni Woods

Feasibility of an advanced thrust termination assembly for a solid propellant rocket motor

NASA Technical Reports Server (NTRS)

1975-01-01

A total of 68 quench tests were conducted in a vented bomb assembly (VBA). Designed to simulate full-scale motor operating conditions, this laboratory apparatus uses a 2-inch-diameter, end-burning propellant charge and an insulated disc of consolidated hydrated aluminum sulfate along with the explosive charge necessary to disperse the salt and inject it onto the burning surface. The VBA was constructed to permit variation of motor design parameters of interest; i.e., weight of salt per unit burning surface area, weight of explosive per unit weight of salt, distance from salt surface to burning surface, incidence angle of salt injection, chamber pressure, and burn time. Completely satisfactory salt quenching, without re-ignition, occurred in only two VBA tests. These were accomplished with a quench charge ratio (QCR) of 0.023 lb salt per square inch of burning surface at dispersing charge ratios (DCR) of 13 and 28 lb of salt per lb of explosive. Candidate materials for insulating salt charges from the rocket combustion environment were evaluated in firings of 5-inch-diameter, uncured end-burner motors. A pressed, alumina ceramic fiber material was selected for further evaluation and use in the final demonstration motor.

KSC-2011-3585

NASA Image and Video Library

2011-05-16

CAPE CANAVERAL, Fla. -- Media capture the launch of space shuttle Endeavour on the STS-134 mission to the International Space Station from the Press Site at NASA's Kennedy Space Center in Florida. The shuttle and its six-member crew lifted off on time at 8:56 a.m. EDT on May 16. STS-134 will deliver the Alpha Magnetic Spectrometer-2 (AMS), Express Logistics Carrier-3, a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the International Space Station. Endeavour's first launch attempt on April 29 was scrubbed because of an issue associated with a faulty power distribution box called the aft load control assembly-2 (ALCA-2). STS-134 will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Troy Cryder

32 CFR 310.10 - General.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) First Amendment rights include, but are not limited to, freedom of religion, freedom of political beliefs, freedom of speech, freedom of the press, the right to assemble, and the right to petition. (f...

Integral gas seal for fuel cell gas distribution assemblies and method of fabrication

DOEpatents

Dettling, Charles J.; Terry, Peter L.

1985-03-19

A porous gas distribution plate assembly for a fuel cell, such as a bipolar assembly, includes an inner impervious region wherein the bipolar assembly has good surface porosity but no through-plane porosity and wherein electrical conductivity through the impervious region is maintained. A hot-pressing process for forming the bipolar assembly includes placing a layer of thermoplastic sealant material between a pair of porous, electrically conductive plates, applying pressure to the assembly at elevated temperature, and allowing the assembly to cool before removing the pressure whereby the layer of sealant material is melted and diffused into the porous plates to form an impervious bond along a common interface between the plates holding the porous plates together. The distribution of sealant within the pores along the surface of the plates provides an effective barrier at their common interface against through-plane transmission of gas.

Method of fabricating an integral gas seal for fuel cell gas distribution assemblies

DOEpatents

Dettling, Charles J.; Terry, Peter L.

1988-03-22

A porous gas distribution plate assembly for a fuel cell, such as a bipolar assembly, includes an inner impervious region wherein the bipolar assembly has good surface porosity but no through-plane porosity and wherein electrical conductivity through the impervious region is maintained. A hot-pressing process for forming the bipolar assembly includes placing a layer of thermoplastic sealant material between a pair of porous, electrically conductive plates, applying pressure to the assembly at elevated temperature, and allowing the assembly to cool before removing the pressure whereby the layer of sealant material is melted and diffused into the porous plates to form an impervious bond along a common interface between the plates holding the porous plates together. The distribution of sealant within the pores along the surface of the plates provides an effective barrier at their common interface against through-plane transmission of gas.

Phylogenomics from Whole Genome Sequences Using aTRAM.

PubMed

Allen, Julie M; Boyd, Bret; Nguyen, Nam-Phuong; Vachaspati, Pranjal; Warnow, Tandy; Huang, Daisie I; Grady, Patrick G S; Bell, Kayce C; Cronk, Quentin C B; Mugisha, Lawrence; Pittendrigh, Barry R; Leonardi, M Soledad; Reed, David L; Johnson, Kevin P

2017-09-01

Novel sequencing technologies are rapidly expanding the size of data sets that can be applied to phylogenetic studies. Currently the most commonly used phylogenomic approaches involve some form of genome reduction. While these approaches make assembling phylogenomic data sets more economical for organisms with large genomes, they reduce the genomic coverage and thereby the long-term utility of the data. Currently, for organisms with moderate to small genomes ($<$1000 Mbp) it is feasible to sequence the entire genome at modest coverage ($10-30\\times$). Computational challenges for handling these large data sets can be alleviated by assembling targeted reads, rather than assembling the entire genome, to produce a phylogenomic data matrix. Here we demonstrate the use of automated Target Restricted Assembly Method (aTRAM) to assemble 1107 single-copy ortholog genes from whole genome sequencing of sucking lice (Anoplura) and out-groups. We developed a pipeline to extract exon sequences from the aTRAM assemblies by annotating them with respect to the original target protein. We aligned these protein sequences with the inferred amino acids and then performed phylogenetic analyses on both the concatenated matrix of genes and on each gene separately in a coalescent analysis. Finally, we tested the limits of successful assembly in aTRAM by assembling 100 genes from close- to distantly related taxa at high to low levels of coverage.Both the concatenated analysis and the coalescent-based analysis produced the same tree topology, which was consistent with previously published results and resolved weakly supported nodes. These results demonstrate that this approach is successful at developing phylogenomic data sets from raw genome sequencing reads. Further, we found that with coverages above $5-10\\times$, aTRAM was successful at assembling 80-90% of the contigs for both close and distantly related taxa. As sequencing costs continue to decline, we expect full genome sequencing will become more feasible for a wider array of organisms, and aTRAM will enable mining of these genomic data sets for an extensive variety of applications, including phylogenomics. [aTRAM; gene assembly; genome sequencing; phylogenomics.]. © The Author(s) 2017. Published by Oxford University Press, on behalf of the Society of Systematic Biologists. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

WE-E-218-01: Writing and Reviewing Papers in Medical Physics.

PubMed

Hendee, W; Slattery, P; Rogers, D; Karellas, A

2012-06-01

There is an art to writing a scientific paper so that it communicates accurately, succinctly, and comprehensively. Developing this art comes with experience, and sharing that experience with younger physicists is an obligation of senior scientists, especially those with editorial responsibilities for the journal. In this workshop, the preparation of a scientific manuscript will be dissected so participants can appreciate how each part is developed and then assembled into a complete paper. Then the review process for the paper will be discussed, including how to examine a paper and write an insightful and constructive review. Finally, we will consider the challenge of accommodating the concerns and recommendations of a reviewer in preparing a revision of the paper. A second feature of the workshop will be a discussion of the process of electronic submission of a paper for consideration by Medical Physics. The web-based PeerX-Press engine for manuscript submission and management will be examined, with attention to special features such as epaps and line-referencing. Finally, new features of Medical Physics will be explained, such as Vision 20/20 manuscripts, Physics Letters and the standardized formatting of book reviews. 1. Improve the participants' abilities to write a scientific manuscript. 2. Understand the review process for Medical Physics manuscripts and how to participate in and benefit from it. 3. Appreciate the many features of the PeerX-Press electronic management process for Medical Physics manuscripts. 4. Develop a knowledge of new features of Medical Physics. © 2012 American Association of Physicists in Medicine.

An improved assembly of the loblolly pine mega-genome using long-read single-molecule sequencing.

PubMed

Zimin, Aleksey V; Stevens, Kristian A; Crepeau, Marc W; Puiu, Daniela; Wegrzyn, Jill L; Yorke, James A; Langley, Charles H; Neale, David B; Salzberg, Steven L

2017-01-01

The 22-gigabase genome of loblolly pine (Pinus taeda) is one of the largest ever sequenced. The draft assembly published in 2014 was built entirely from short Illumina reads, with lengths ranging from 100 to 250 base pairs (bp). The assembly was quite fragmented, containing over 11 million contigs whose weighted average (N50) size was 8206 bp. To improve this result, we generated approximately 12-fold coverage in long reads using the Single Molecule Real Time sequencing technology developed at Pacific Biosciences. We assembled the long and short reads together using the MaSuRCA mega-reads assembly algorithm, which produced a substantially better assembly, P. taeda version 2.0. The new assembly has an N50 contig size of 25 361, more than three times as large as achieved in the original assembly, and an N50 scaffold size of 107 821, 61% larger than the previous assembly. © The Author 2017. Published by Oxford University Press.

Comprehensive evaluation of non-hybrid genome assembly tools for third-generation PacBio long-read sequence data.

PubMed

Jayakumar, Vasanthan; Sakakibara, Yasubumi

2017-11-03

Long reads obtained from third-generation sequencing platforms can help overcome the long-standing challenge of the de novo assembly of sequences for the genomic analysis of non-model eukaryotic organisms. Numerous long-read-aided de novo assemblies have been published recently, which exhibited superior quality of the assembled genomes in comparison with those achieved using earlier second-generation sequencing technologies. Evaluating assemblies is important in guiding the appropriate choice for specific research needs. In this study, we evaluated 10 long-read assemblers using a variety of metrics on Pacific Biosciences (PacBio) data sets from different taxonomic categories with considerable differences in genome size. The results allowed us to narrow down the list to a few assemblers that can be effectively applied to eukaryotic assembly projects. Moreover, we highlight how best to use limited genomic resources for effectively evaluating the genome assemblies of non-model organisms. © The Author 2017. Published by Oxford University Press.

Erratum to: An improved assembly of the loblolly pine mega-genome using long-read single-molecule sequencing.

PubMed

Zimin, Aleksey V; Stevens, Kristian A; Crepeau, Marc W; Puiu, Daniela; Wegrzyn, Jill L; Yorke, James A; Langley, Charles H; Neale, David B; Salzberg, Steven L

2017-10-01

The 22-gigabase genome of loblolly pine (Pinus taeda) is one of the largest ever sequenced. The draft assembly published in 2014 was built entirely from short Illumina reads, with lengths ranging from 100 to 250 base pairs (bp). The assembly was quite fragmented, containing over 11 million contigs whose weighted average (N50) size was 8206 bp. To improve this result, we generated approximately 12-fold coverage in long reads using the Single Molecule Real Time sequencing technology developed at Pacific Biosciences. We assembled the long and short reads together using the MaSuRCA mega-reads assembly algorithm, which produced a substantially better assembly, P. taeda version 2.0. The new assembly has an N50 contig size of 25 361, more than three times as large as achieved in the original assembly, and an N50 scaffold size of 107 821, 61% larger than the previous assembly. © The Authors 2017. Published by Oxford University Press.

4 CFR 83.10 - First Amendment rights.

Code of Federal Regulations, 2014 CFR

2014-01-01

... enforcement activity. These rights include, but are not limited to, free exercise of religious and political beliefs, freedom of speech and the press, and freedom to assemble and to petition the Government. ...

Work-related musculoskeletal disorders (WMDs) risk assessment at core assembly production of electronic components manufacturing company

NASA Astrophysics Data System (ADS)

Yahya, N. M.; Zahid, M. N. O.

2018-03-01

This study conducted to assess the work-related musculoskeletal disorders (WMDs) among the workers at core assembly production in an electronic components manufacturing company located in Pekan, Pahang, Malaysia. The study is to identify the WMDs risk factor and risk level. A set of questionnaires survey based on modified Nordic Musculoskeletal Disorder Questionnaires have been distributed to respective workers to acquire the WMDs risk factor identification. Then, postural analysis was conducted in order to measure the respective WMDs risk level. The analysis were based on two ergonomics assessment tools; Rapid Upper Limb Assessment (RULA) and Rapid Entire Body Assessment (REBA). The study found that 30 respondents out of 36 respondents suffered from WMDs especially at shoulder, wrists and lower back. The WMDs risk have been identified from unloading process, pressing process and winding process. In term of the WMDs risk level, REBA and RULA assessment tools have indicated high risk level to unloading and pressing process. Thus, this study had established the WMDs risk factor and risk level of core assembly production in an electronic components manufacturing company at Malaysia environment.

Human Rights and U.S. Foreign Policy in North Korea

DTIC Science & Technology

1999-12-01

that are occurring in North Korea today. One freedom that is infringed upon regularly by the regime is the freedom of speech . The State Department’s...example, article 67 guarantees the freedom of speech , press, assembly, demonstration and association. Article 68 guarantees the freedom of religion...NSL, 42 movement toward reform has been very slow. As with the D.P.R.K., the R.O.K. constitution also provides the freedoms of speech , press

Recent advances in sequence assembly: principles and applications.

PubMed

Chen, Qingfeng; Lan, Chaowang; Zhao, Liang; Wang, Jianxin; Chen, Baoshan; Chen, Yi-Ping Phoebe

2017-11-01

The application of advanced sequencing technologies and the rapid growth of various sequence data have led to increasing interest in DNA sequence assembly. However, repeats and polymorphism occur frequently in genomes, and each of these has different impacts on assembly. Further, many new applications for sequencing, such as metagenomics regarding multiple species, have emerged in recent years. These not only give rise to higher complexity but also prevent short-read assembly in an efficient way. This article reviews the theoretical foundations that underlie current mapping-based assembly and de novo-based assembly, and highlights the key issues and feasible solutions that need to be considered. It focuses on how individual processes, such as optimal k-mer determination and error correction in assembly, rely on intelligent strategies or high-performance computation. We also survey primary algorithms/software and offer a discussion on the emerging challenges in assembly. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.

75 FR 59212 - Certain Coated Paper Suitable for High-Quality Print Graphics Using Sheet-Fed Presses From the...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-27

... Suitable for High-Quality Print Graphics Using Sheet-Fed Presses From the People's Republic of China: Final... suitable for high-quality print graphics using sheet-fed presses from the People's Republic of China (``PRC...-Fed Presses from the People's Republic of China: Preliminary Affirmative Countervailing Duty...

Draft genome of the sea cucumber Apostichopus japonicus and genetic polymorphism among color variants.

PubMed

Jo, Jihoon; Oh, Jooseong; Lee, Hyun-Gwan; Hong, Hyun-Hee; Lee, Sung-Gwon; Cheon, Seongmin; Kern, Elizabeth M A; Jin, Soyeong; Cho, Sung-Jin; Park, Joong-Ki; Park, Chungoo

2017-01-01

The Japanese sea cucumber (Apostichopus japonicus Selenka 1867) is an economically important species as a source of seafood and ingredient in traditional medicine. It is mainly found off the coasts of northeast Asia. Recently, substantial exploitation and widespread biotic diseases in A. japonicus have generated increasing conservation concern. However, the genomic knowledge base and resources available for researchers to use in managing this natural resource and to establish genetically based breeding systems for sea cucumber aquaculture are still in a nascent stage. A total of 312 Gb of raw sequences were generated using the Illumina HiSeq 2000 platform and assembled to a final size of 0.66 Gb, which is about 80.5% of the estimated genome size (0.82 Gb). We observed nucleotide-level heterozygosity within the assembled genome to be 0.986%. The resulting draft genome assembly comprising 132 607 scaffolds with an N50 value of 10.5 kb contains a total of 21 771 predicted protein-coding genes. We identified 6.6-14.5 million heterozygous single nucleotide polymorphisms in the assembled genome of the three natural color variants (green, red, and black), resulting in an estimated nucleotide diversity of 0.00146. We report the first draft genome of A. japonicus and provide a general overview of the genetic variation in the three major color variants of A. japonicus. These data will help provide a comprehensive view of the genetic, physiological, and evolutionary relationships among color variants in A. japonicus, and will be invaluable resources for sea cucumber genomic research. © The Author 2017. Published by Oxford University Press.

Sub-Saharan Africa Report.

DTIC Science & Technology

1985-12-27

8217 — told the assembled Press that* *the proposed legislation did not’. :■ cover agricultural Workers or’. i, domestic employee^ two of the I • largest...8217, isting legislation only, covers a^ j small minority öf workers,., primarily in the building,„ ,-manüfacturing and mining ’ sectors...presented to the National Assembly for debate during the current legislative , session, which began last week. However, there is no sign as yet of the

In vitro reconstitution of chaperone-mediated human RISC assembly.

PubMed

Naruse, Ken; Matsuura-Suzuki, Eriko; Watanabe, Mariko; Iwasaki, Shintaro; Tomari, Yukihide

2018-01-01

To silence target mRNAs, small RNAs and Argonaute (Ago) proteins need to be assembled into RNA-induced silencing complexes (RISCs). Although the assembly of Drosophila melanogaster RISC was recently reconstituted by Ago2, the Dicer-2/R2D2 heterodimer, and five chaperone proteins, the absence of a reconstitution system for mammalian RISC assembly has posed analytical challenges. Here we describe reconstitution of human RISC assembly using Ago2 and five recombinant chaperone proteins: Hsp90β, Hsc70, Hop, Dnaja2, and p23. Our data show that ATP hydrolysis by both Hsp90β and Hsc70 is required for RISC assembly of small RNA duplexes but not for that of single-stranded RNAs. The reconstitution system lays the groundwork for further studies of small RNA-mediated gene silencing in mammals. © 2018 Naruse et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

Optical Assembly and Characterization System for Nano-Photonics Research

DTIC Science & Technology

2016-03-01

Unlimited Final Report: Optical Assembly and Characterization System for Nano -Photonics Research The views, opinions and/or findings contained in this...reviewed journals: Final Report: Optical Assembly and Characterization System for Nano -Photonics Research Report Title With this equipment funding support...Assembly and Characterization System for Nano -Photonics Research PI: Prof. Weidong Zhou, University of Texas at Arlington (UTA) 500 S. Cooper St

Commercial landscape of noninvasive prenatal testing in the United States.

PubMed

Agarwal, Ashwin; Sayres, Lauren C; Cho, Mildred K; Cook-Deegan, Robert; Chandrasekharan, Subhashini

2013-06-01

Cell-free fetal DNA-based noninvasive prenatal testing (NIPT) could significantly change the paradigm of prenatal testing and screening. Intellectual property (IP) and commercialization promise to be important components of the emerging debate about clinical implementation of these technologies. We have assembled information about types of testing, prices, turnaround times, and reimbursement of recently launched commercial tests in the United States from the trade press, news articles, and scientific, legal, and business publications. We also describe the patenting and licensing landscape of technologies underlying these tests and ongoing patent litigation in the United States. Finally, we discuss how IP issues may affect clinical translation of NIPT and their potential implications for stakeholders. Fetal medicine professionals (clinicians and researchers), genetic counselors, insurers, regulators, test developers, and patients may be able to use this information to make informed decisions about clinical implementation of current and emerging noninvasive prenatal tests. © 2013 John Wiley & Sons, Ltd.

Commercial Landscape of noninvasive prenatal testing in the United States

PubMed Central

Agarwal, Ashwin; Sayres, Lauren C.; Cho, Mildred K.; Cook-Deegan, Robert; Chandrasekharan, Subhashini

2014-01-01

Cell-free fetal DNA-based noninvasive prenatal testing (NIPT) could significantly change the paradigm of prenatal testing and screening. Intellectual property (IP) and commercialization promise to be important components of the emerging debate about clinical implementation of these technologies. We have assembled information about types of testing, prices, turnaround times and reimbursement of recently launched commercial tests in the United States from the trade press, news articles, and scientific, legal, and business publications. We also describe the patenting and licensing landscape of technologies underlying these tests and ongoing patent litigation in the United States. Finally, we discuss how IP issues may affect clinical translation of NIPT and their potential implications for stakeholders. Fetal medicine professionals (clinicians and researchers), genetic counselors, insurers, regulators, test developers and patients may be able to use this information to make informed decisions about clinical implementation of current and emerging noninvasive prenatal tests. PMID:23686656

Research to Assembly Scheme for Satellite Deck Based on Robot Flexibility Control Principle

NASA Astrophysics Data System (ADS)

Guo, Tao; Hu, Ruiqin; Xiao, Zhengyi; Zhao, Jingjing; Fang, Zhikai

2018-03-01

Deck assembly is critical quality control point in final satellite assembly process, and cable extrusion and structure collision problems in assembly process will affect development quality and progress of satellite directly. Aimed at problems existing in deck assembly process, assembly project scheme for satellite deck based on robot flexibility control principle is proposed in this paper. Scheme is introduced firstly; secondly, key technologies on end force perception and flexible docking control in the scheme are studied; then, implementation process of assembly scheme for satellite deck is described in detail; finally, actual application case of assembly scheme is given. Result shows that compared with traditional assembly scheme, assembly scheme for satellite deck based on robot flexibility control principle has obvious advantages in work efficiency, reliability and universality aspects etc.

Performance of polymer nano composite membrane electrode assembly using Alginate as a dopant in polymer electrolyte membrane fuel cell

NASA Astrophysics Data System (ADS)

Mulijani, S.

2017-01-01

Polymer membrane and composite polymer for membrane electrode assembly (MEAs) are synthesized and studied for usage in direct methanol fuel cell (DMFC). In this study, we prepared 3 type of MEAs, polystyrene (PS), sulfonated polystyrene (SPS) and composite polymer SPS-alginat membrane via catalyst hot pressed method. The performance and properties of prepared MEAs were evaluated and analyzed by impedance spectrometry and scanning electron microscopy (SEM). The result showed that, water up take of MEA composite polymer SPS-alginate was obtained higher than that in SPS and PS. The proton conductivity of MEA-SPS-alginate was also higher than that PS and PSS. SEM characterization revealed that the intimate contact between the carbon catalyst layers (CL) and the membranes, and the uniformly porous structure correlate positively with the MEAs prepared by hot pressed method, exhibiting high performances for DMFC.

When less is more: 'slicing' sequencing data improves read decoding accuracy and de novo assembly quality.

PubMed

Lonardi, Stefano; Mirebrahim, Hamid; Wanamaker, Steve; Alpert, Matthew; Ciardo, Gianfranco; Duma, Denisa; Close, Timothy J

2015-09-15

As the invention of DNA sequencing in the 70s, computational biologists have had to deal with the problem of de novo genome assembly with limited (or insufficient) depth of sequencing. In this work, we investigate the opposite problem, that is, the challenge of dealing with excessive depth of sequencing. We explore the effect of ultra-deep sequencing data in two domains: (i) the problem of decoding reads to bacterial artificial chromosome (BAC) clones (in the context of the combinatorial pooling design we have recently proposed), and (ii) the problem of de novo assembly of BAC clones. Using real ultra-deep sequencing data, we show that when the depth of sequencing increases over a certain threshold, sequencing errors make these two problems harder and harder (instead of easier, as one would expect with error-free data), and as a consequence the quality of the solution degrades with more and more data. For the first problem, we propose an effective solution based on 'divide and conquer': we 'slice' a large dataset into smaller samples of optimal size, decode each slice independently, and then merge the results. Experimental results on over 15 000 barley BACs and over 4000 cowpea BACs demonstrate a significant improvement in the quality of the decoding and the final assembly. For the second problem, we show for the first time that modern de novo assemblers cannot take advantage of ultra-deep sequencing data. Python scripts to process slices and resolve decoding conflicts are available from http://goo.gl/YXgdHT; software Hashfilter can be downloaded from http://goo.gl/MIyZHs stelo@cs.ucr.edu or timothy.close@ucr.edu Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

The Hot-Pressing of Hafnium Carbide (Melting Point, 7030 F)

NASA Technical Reports Server (NTRS)

Sanders, William A.; Grisaffe, Salvatore J.

1960-01-01

An investigation was undertaken to determine the effects of the hot-pressing variables (temperature, pressure, and time) on the density and grain size of hafnium carbide disks. The purpose was to provide information necessary for the production of high-density test shapes for the determination of physical and mechanical properties. Hot-pressing of -325 mesh hafnium carbide powder was accomplished with a hydraulic press and an inductively heated graphite die assembly. The ranges investigated for each variable were as follows: temperature, 3500 to 4870 F; pressure, 1000 to 6030 pounds per square inch; and time, 5 to 60 minutes. Hafnium carbide bodies of approximately 98 percent theoretical density can be produced under the following minimal conditions: 4230 F, 3500 pounds per square inch, and 15 minutes. Further increases in temperature and time resulted only in greater grain size.

KAT: a K-mer analysis toolkit to quality control NGS datasets and genome assemblies.

PubMed

Mapleson, Daniel; Garcia Accinelli, Gonzalo; Kettleborough, George; Wright, Jonathan; Clavijo, Bernardo J

2017-02-15

De novo assembly of whole genome shotgun (WGS) next-generation sequencing (NGS) data benefits from high-quality input with high coverage. However, in practice, determining the quality and quantity of useful reads quickly and in a reference-free manner is not trivial. Gaining a better understanding of the WGS data, and how that data is utilized by assemblers, provides useful insights that can inform the assembly process and result in better assemblies. We present the K-mer Analysis Toolkit (KAT): a multi-purpose software toolkit for reference-free quality control (QC) of WGS reads and de novo genome assemblies, primarily via their k-mer frequencies and GC composition. KAT enables users to assess levels of errors, bias and contamination at various stages of the assembly process. In this paper we highlight KAT's ability to provide valuable insights into assembly composition and quality of genome assemblies through pairwise comparison of k-mers present in both input reads and the assemblies. KAT is available under the GPLv3 license at: https://github.com/TGAC/KAT . bernardo.clavijo@earlham.ac.uk. Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press.

Expedite the Processing of Unpressurized Payloads to the International Space Station Using the ExPRESS Pallet

NASA Technical Reports Server (NTRS)

Bacskay, Allen S.; Gilbert, Paul A. (Technical Monitor)

2002-01-01

The Expedite the PRocessing of Experiments to Space Station (ExPRESS) Pallet will be used as an experiment platform for external/unpressurized payloads to be flown aboard the International Space Station (ISS). The purpose of the ExPRESS Pallet is to provide an easy access to the ISS for Scientific Investigators that require an external platform for their experiment hardware. As the name of the ExPRESS Pallet implies, the objective of the ExPRESS program is to provide a simplified integration process in a short time period (24 months) for payloads to be flown on the ISS. The ExPRESS Pallet provides unique opportunities for research across many science disciplines, including earth observation, communications, solar and deep space viewing, and long-term exposure. The ExPRESS Pallet provides access to Ram, Wake, Nadir, Zenith and Earth Limb for viewing and exposure to the space environment. The ExPRESS Pallet will provide standard physical payload interfaces, and a standard integration template. The ExPS consists of the Pallet structure, payload Adapters, a subsystem assembly that includes data controller, power distribution and conversion, and Extra Vehicular Robotics compatibility. The ExPRESS Pallet provides the capability to changeout payloads on-orbit via the ExPRESS Pallet Adapter (ExPA). The following paragraphs will describe the Services and Accommodations available to the Payload developers by the ExPRESS Pallet and a brief description of the Integration process. More detailed information on the ExPRESS Pallet can be found in the ExPRESS Pallet Payload Accommodations Handbook, SSP 52000-PAH-EPP.

Method of joining ceramics

DOEpatents

Henager, Jr., Charles H.; Brimhall, John L.

2000-01-01

According to the method of the present invention, joining a first bi-element carbide to a second bi-element carbide, has the steps of: (a) forming a bond agent containing a metal carbide and silicon; (b) placing the bond agent between the first and second bi-element carbides to form a pre-assembly; and (c) pressing and heating the pre-assembly in a non-oxidizing atmosphere to a temperature effective to induce a displacement reaction creating a metal silicon phase bonding the first and second bi-element carbides.

The mechanisms for nanoparticle surface diffusion and chain self-assembly determined from real-time nanoscale kinetics in liquid

DOE PAGES

Woehl, Taylor J.; Prozorov, Tanya

2015-08-20

The mechanisms for nanoparticle self-assembly are often inferred from the morphology of the final nanostructures in terms of attractive and repulsive interparticle interactions. Understanding how nanoparticle building blocks are pieced together during self-assembly is a key missing component needed to unlock new strategies and mechanistic understanding of this process. Here we use real-time nanoscale kinetics derived from liquid cell transmission electron microscopy investigation of nanoparticle self-assembly to show that nanoparticle mobility dictates the pathway for self-assembly and final nanostructure morphology. We describe a new method for modulating nanoparticle diffusion in a liquid cell, which we employ to systematically investigate themore » effect of mobility on self-assembly of nanoparticles. We interpret the observed diffusion in terms of electrostatically induced surface diffusion resulting from nanoparticle hopping on the liquid cell window surface. Slow-moving nanoparticles self-assemble predominantly into linear 1D chains by sequential attachment of nanoparticles to existing chains, while highly mobile nanoparticles self-assemble into chains and branched structures by chain–chain attachments. Self-assembly kinetics are consistent with a diffusion-driven mechanism; we attribute the change in self-assembly pathway to the increased self-assembly rate of highly mobile nanoparticles. Furthermore, these results indicate that nanoparticle mobility can dictate the self-assembly mechanism and final nanostructure morphology in a manner similar to interparticle interactions.« less

Uzbekistan: Recent Developments and U.S. Interests

DTIC Science & Technology

2005-05-02

the December 26, 2004, Legislative Chamber elections, the government restricted freedom of speech and assembly. In February 2004, the government...5,500. The government severely restricted freedom of speech and the press and continued to prohibit unauthorized public meetings and demonstrations

Cryogenically cooled detector pin mount

DOEpatents

Hunt, Jr., William E; Chrisp, Michael P

2014-06-03

A focal plane assembly facilitates a molybdenum base plate being mounted to another plate made from aluminum. The molybdenum pin is an interference fit (press fit) in the aluminum base plate. An annular cut out area in the base plate forms two annular flexures.

Senegal: Background and U.S. Relations

DTIC Science & Technology

2012-02-20

press, and assembly; corruption and impunity; rape, domestic violence, sexual harassment of and discrimination against women; female genital mutilation ...Senegal’s Budget Process is Transparent [UNCLASSIFIED],” May 4, 2010. 23 The 1999 Penal Law outlawed domestic violence and female genital mutilation , and

18 CFR 3b.201 - Content of records.

Code of Federal Regulations, 2010 CFR

2010-04-01

..., freedom of speech and of the press, and freedom of assembly and petition. In determining whether or not a... member of the public under the Freedom of Information Act, 5 U.S.C. 552, as amended, or to another agency...

MetaQUAST: evaluation of metagenome assemblies.

PubMed

Mikheenko, Alla; Saveliev, Vladislav; Gurevich, Alexey

2016-04-01

During the past years we have witnessed the rapid development of new metagenome assembly methods. Although there are many benchmark utilities designed for single-genome assemblies, there is no well-recognized evaluation and comparison tool for metagenomic-specific analogues. In this article, we present MetaQUAST, a modification of QUAST, the state-of-the-art tool for genome assembly evaluation based on alignment of contigs to a reference. MetaQUAST addresses such metagenome datasets features as (i) unknown species content by detecting and downloading reference sequences, (ii) huge diversity by giving comprehensive reports for multiple genomes and (iii) presence of highly relative species by detecting chimeric contigs. We demonstrate MetaQUAST performance by comparing several leading assemblers on one simulated and two real datasets. http://bioinf.spbau.ru/metaquast aleksey.gurevich@spbu.ru Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Modeling biological problems in computer science: a case study in genome assembly.

PubMed

Medvedev, Paul

2018-01-30

As computer scientists working in bioinformatics/computational biology, we often face the challenge of coming up with an algorithm to answer a biological question. This occurs in many areas, such as variant calling, alignment and assembly. In this tutorial, we use the example of the genome assembly problem to demonstrate how to go from a question in the biological realm to a solution in the computer science realm. We show the modeling process step-by-step, including all the intermediate failed attempts. Please note this is not an introduction to how genome assembly algorithms work and, if treated as such, would be incomplete and unnecessarily long-winded. © The Author(s) 2018. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

75 FR 59223 - Certain Coated Paper Suitable for High-Quality Print Graphics Using Sheet-Fed Presses From...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-27

... Suitable for High-Quality Print Graphics Using Sheet-Fed Presses From Indonesia: Final Determination of... high-quality print graphics using sheet-fed presses (certain coated paper) from Indonesia is being, or... certain coated paper from Indonesia. See Certain Coated Paper Suitable for High-Quality Print Graphics...

Assembling short reads from jumping libraries with large insert sizes.

PubMed

Vasilinetc, Irina; Prjibelski, Andrey D; Gurevich, Alexey; Korobeynikov, Anton; Pevzner, Pavel A

2015-10-15

Advances in Next-Generation Sequencing technologies and sample preparation recently enabled generation of high-quality jumping libraries that have a potential to significantly improve short read assemblies. However, assembly algorithms have to catch up with experimental innovations to benefit from them and to produce high-quality assemblies. We present a new algorithm that extends recently described exSPAnder universal repeat resolution approach to enable its applications to several challenging data types, including jumping libraries generated by the recently developed Illumina Nextera Mate Pair protocol. We demonstrate that, with these improvements, bacterial genomes often can be assembled in a few contigs using only a single Nextera Mate Pair library of short reads. Described algorithms are implemented in C++ as a part of SPAdes genome assembler, which is freely available at bioinf.spbau.ru/en/spades. ap@bioinf.spbau.ru Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Press conference with the crew of the 41-G mission

NASA Image and Video Library

1984-09-12

S84-41580 (3 Sept 1984) --- Assembled together publicly for the first time, the seven crewmembers for NASA's 41-G Space Shuttle mission field questions from the press corps at the Johnson Space Center. Pictured (foreground right to left) are Robert L. Crippen, crew commander ;Jon A. McBride, pilot; Kathryn D. Sullivan, Sally K. Ride and David C. Leestma--all mission specialists; Marc Garneau, representing the Canadian National Research Council, and Paul D. Scully-Power, U.S. Navy oceanographer, both payload specialists. Their flight is scheduled for early October.

Thermoelectric-figure-of-merit enhancement of silicon-germanium through nanocomposite concept

NASA Astrophysics Data System (ADS)

Wang, Dezhi

SiGe alloy has been the thermoelectric material element of RTGs (Radioisotope thermoelectric power generators) for more than 20 years because of its good performance at high temperature. It also has a very high potential application in converting exhaust heat into useful electricity, which currently attracts a lot of research interest in the automotive industry where 40% of the energy was rejected as exhaust heat. However, its low conversion efficiency (8%) is a major concern although it is the best in practice. A new concept, namely Si-Ge nanocomposite, was proposed to enhance thermoelectric figure-of-merit. Fast heating pressure sintering was found to be an appropriate synthesizing method and a lab-made direct current-induced hot press system was established. It can reach l200°C within several minutes and many parameters can be controlled. The uniquely designed graphite die assembly can stand l60MPa pressure which is better than the best commercial products (127MPa). Numerous Si-Ge nanocomposite samples were pressed using our DC hot press. Fully dense n-type Si-Ge nanocomposite samples of nanoSi80nanoGe20P were finally obtained. The nanocomposite structure was characterized via XRD, SEM, EDS, and TEM. The proposed nanocomposite structure, dots in a matrix, was observed. Most importantly, the thermoelectric property measurements showed that the Si-Ge nanocomposite of n-type nanoSi80nanoGe20 possessed higher electrical conductivity but lower thermal conductivity, thus a higher ZT than that of n-type nanoSi80microGe20. This result proved that thermoelectric-figure-of-merit enhancement through the nanocomposite concept was the right direction.

Automatic assembly of micro-optical components

NASA Astrophysics Data System (ADS)

Gengenbach, Ulrich K.

1996-12-01

Automatic assembly becomes an important issue as hybrid micro systems enter industrial fabrication. Moving from a laboratory scale production with manual assembly and bonding processes to automatic assembly requires a thorough re- evaluation of the design, the characteristics of the individual components and of the processes involved. Parts supply for automatic operation, sensitive and intelligent grippers adapted to size, surface and material properties of the microcomponents gain importance when the superior sensory and handling skills of a human are to be replaced by a machine. This holds in particular for the automatic assembly of micro-optical components. The paper outlines these issues exemplified at the automatic assembly of a micro-optical duplexer consisting of a micro-optical bench fabricated by the LIGA technique, two spherical lenses, a wavelength filter and an optical fiber. Spherical lenses, wavelength filter and optical fiber are supplied by third party vendors, which raises the question of parts supply for automatic assembly. The bonding processes for these components include press fit and adhesive bonding. The prototype assembly system with all relevant components e.g. handling system, parts supply, grippers and control is described. Results of first automatic assembly tests are presented.

STS-49 Endeavour/Intelsat Briefing

NASA Technical Reports Server (NTRS)

1992-01-01

Lak Virdee of Intelsat, summarizes Intelsat's role in the STS-49 Endeavour mission. He discusses the reboost hardware, giving details on the capture arm and docker adapter assembly. He describes the rendezvous between Intelsat and the Endeavour Orbiter. Mr. Virdee then answers questions from the press.

Shuttle S-band high gain switched beam breadboard antennas

NASA Technical Reports Server (NTRS)

Mullaney, J. J.

1985-01-01

The final fabrication and assembly of the S-band five-element, eight-beam breadboard antennas developed for the Space Shuttle program are described. Data summary sheets from component and final assembly testing are presented.

Study of the Antitoxic Effect of Unithiol on Cystamine in Dogs

DTIC Science & Technology

2008-08-01

Ionizing Radiation with the Aid of Unithiol. Radiazionnaya biologia . Radioecologia, 34(3): 424-429, 1994. 15a. Grachev S.A., Sverdlov A.G., Nikianorova N.G...and Timoshenko S.I., Influence of Unithiol on Toxic Effects of Cvstamine in Dogs. Radiazionnaya biologia . Radioecologia, 1998. (in press when final...Protection Against Fission Neutrons Using Unithiol. Radiazionnaya biologia . Radioecologia, 1998. (in press when final document submitted to DSWA). 16

KSC-2011-5246

NASA Image and Video Library

2011-07-08

CAPE CANAVERAL, Fla. -- Media from around the globe gather on the grounds of the Press Site at NASA's Kennedy Space Center in Florida to photograph and cover the prelaunch activities and lift off of space shuttle Atlantis on its STS-135 mission to the International Space Station. Seen towering above is the massive Vehicle Assembly Building. Dozens of satellite news vehicles and trailers can be seen in the parking lot. In the background is the Turn Basin where NASA's Pegasus barge delivered the final external tank for the mission. Atlantis began its final flight, with Commander Chris Ferguson, Pilot Doug Hurley and Mission Specialists Sandy Magnus and Rex Walheim on board, at 11:29 a.m. EDT July 8 to deliver the Raffaello multi-purpose logistics module packed with supplies and spare parts to the station. Also in Atlantis' payload bay is the Robotic Refueling Mission experiment that will investigate the potential for robotically refueling existing satellites in orbit. In addition, Atlantis will return with a failed ammonia pump module to help NASA better understand the failure mechanism and improve pump designs for future systems. STS-135 is the 33rd flight of Atlantis, the 37th shuttle mission to the space station, and the 135th and final mission of NASA's Space Shuttle Program. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-5245

NASA Image and Video Library

2011-07-08

CAPE CANAVERAL, Fla. -- Media from around the globe gather on the grounds of the Press Site at NASA's Kennedy Space Center in Florida to photograph and cover the prelaunch activities and lift off of space shuttle Atlantis on its STS-135 mission to the International Space Station. Seen towering above is the massive Vehicle Assembly Building. Dozens of satellite news vehicles and trailers can be seen in the parking lot. In the background is the Turn Basin where NASA's Pegasus barge delivered the final external tank for the mission. Atlantis began its final flight, with Commander Chris Ferguson, Pilot Doug Hurley and Mission Specialists Sandy Magnus and Rex Walheim on board, at 11:29 a.m. EDT July 8 to deliver the Raffaello multi-purpose logistics module packed with supplies and spare parts to the station. Also in Atlantis' payload bay is the Robotic Refueling Mission experiment that will investigate the potential for robotically refueling existing satellites in orbit. In addition, Atlantis will return with a failed ammonia pump module to help NASA better understand the failure mechanism and improve pump designs for future systems. STS-135 is the 33rd flight of Atlantis, the 37th shuttle mission to the space station, and the 135th and final mission of NASA's Space Shuttle Program. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Jim Grossmann

A New Vision for the First Amendment in Schools.

ERIC Educational Resources Information Center

Chaltain, Sam

2002-01-01

Describes the First Amendment Schools project aimed at teaching K-12 public and independent school students their constitutionally protected religious, speech, press, assembly, and petition rights and responsibilities. Includes examples describing the project in several schools. Includes annotated list of resources for educators. (PKP)

Printing/Graphic Arts Technology Program Guide.

ERIC Educational Resources Information Center

Georgia Univ., Athens. Dept. of Vocational Education.

This guide presents the standard curriculum for technical institutes in Georgia. The curriculum addresses the minimum competencies for a printing/graphics technology program. It includes specializations in art and copy preparation, prepress/image assembly, and lithographic press operations. The guide contains four major sections. The General…

The present and future of de novo whole-genome assembly.

PubMed

Sohn, Jang-Il; Nam, Jin-Wu

2018-01-01

As the advent of next-generation sequencing (NGS) technology, various de novo assembly algorithms based on the de Bruijn graph have been developed to construct chromosome-level sequences. However, numerous technical or computational challenges in de novo assembly still remain, although many bright ideas and heuristics have been suggested to tackle the challenges in both experimental and computational settings. In this review, we categorize de novo assemblers on the basis of the type of de Bruijn graphs (Hamiltonian and Eulerian) and discuss the challenges of de novo assembly for short NGS reads regarding computational complexity and assembly ambiguity. Then, we discuss how the limitations of the short reads can be overcome by using a single-molecule sequencing platform that generates long reads of up to several kilobases. In fact, the long read assembly has caused a paradigm shift in whole-genome assembly in terms of algorithms and supporting steps. We also summarize (i) hybrid assemblies using both short and long reads and (ii) overlap-based assemblies for long reads and discuss their challenges and future prospects. This review provides guidelines to determine the optimal approach for a given input data type, computational budget or genome. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Metagenomic assembly through the lens of validation: recent advances in assessing and improving the quality of genomes assembled from metagenomes.

PubMed

Olson, Nathan D; Treangen, Todd J; Hill, Christopher M; Cepeda-Espinoza, Victoria; Ghurye, Jay; Koren, Sergey; Pop, Mihai

2017-08-07

Metagenomic samples are snapshots of complex ecosystems at work. They comprise hundreds of known and unknown species, contain multiple strain variants and vary greatly within and across environments. Many microbes found in microbial communities are not easily grown in culture making their DNA sequence our only clue into their evolutionary history and biological function. Metagenomic assembly is a computational process aimed at reconstructing genes and genomes from metagenomic mixtures. Current methods have made significant strides in reconstructing DNA segments comprising operons, tandem gene arrays and syntenic blocks. Shorter, higher-throughput sequencing technologies have become the de facto standard in the field. Sequencers are now able to generate billions of short reads in only a few days. Multiple metagenomic assembly strategies, pipelines and assemblers have appeared in recent years. Owing to the inherent complexity of metagenome assembly, regardless of the assembly algorithm and sequencing method, metagenome assemblies contain errors. Recent developments in assembly validation tools have played a pivotal role in improving metagenomics assemblers. Here, we survey recent progress in the field of metagenomic assembly, provide an overview of key approaches for genomic and metagenomic assembly validation and demonstrate the insights that can be derived from assemblies through the use of assembly validation strategies. We also discuss the potential for impact of long-read technologies in metagenomics. We conclude with a discussion of future challenges and opportunities in the field of metagenomic assembly and validation. © The Author 2017. Published by Oxford University Press.

Batch Scheduling for Hybrid Assembly Differentiation Flow Shop to Minimize Total Actual Flow Time

NASA Astrophysics Data System (ADS)

Maulidya, R.; Suprayogi; Wangsaputra, R.; Halim, A. H.

2018-03-01

A hybrid assembly differentiation flow shop is a three-stage flow shop consisting of Machining, Assembly and Differentiation Stages and producing different types of products. In the machining stage, parts are processed in batches on different (unrelated) machines. In the assembly stage, each part of the different parts is assembled into an assembly product. Finally, the assembled products will further be processed into different types of final products in the differentiation stage. In this paper, we develop a batch scheduling model for a hybrid assembly differentiation flow shop to minimize the total actual flow time defined as the total times part spent in the shop floor from the arrival times until its due date. We also proposed a heuristic algorithm for solving the problems. The proposed algorithm is tested using a set of hypothetic data. The solution shows that the algorithm can solve the problems effectively.

Thermal Interference Fit Anode Assembly for Cathodic Protection

DTIC Science & Technology

2018-02-22

than an inside diameter of the cylindrical anode, is subject to the low temperature for a minimum of six hours and preferably twenty- four hours to...degrees Fahrenheit after a minimum of six hours and preferably twenty-four hours. The anode 10 is immediately fused to the mounting bolt 20 by hand...degrees Fahrenheit after a minimum of six hours and preferably twenty-four hours. The anode is then hand pressed onto the mounting bolt to bottom out on the non-anodized face in order to form a single fused assembly.

Thermoelectric generator with hinged assembly for fins

DOEpatents

Purdy, David L.; Shapiro, Zalman M.; Hursen, Thomas F.; Maurer, Gerould W.

1976-11-02

A cylindrical casing has a central shielded capsule of radioisotope fuel. A plurality of thermonuclear modules are axially arranged with their hot junctions resiliently pressed toward the shield and with their cold junctions adjacent a transition member having fins radiating heat to the environment. For each module, the assembly of transition member and fins is hinged to the casing for swinging to permit access to and removal of such module. A ceramic plate having gold layers on opposite faces prevents diffusion bonding of the hot junction to the shield.

Hardwood press-lam crossties : processing and performance

Treesearch

J.L. Tschernitz; E.L. Schaffer; R.C. Moody; R.W. Jokerst; D.S. Gromala; C.C. Peters; W.T. Henry

1979-01-01

Crossties were made by the Press-Lam process, in which logs are peeled into veneers, dried, and glued into billets in a continuous procedure. Billets were made from 8.5-foot-long veneers and from 4-foot-long veneers, cut into final product dimensions, and treated with preservative. In laboratory tests, bending strength and stiffness of the Press-Lam ties were found to...

Cortical bone viscoelasticity and fixation strength of press-fit femoral stems: an in-vitro model.

PubMed

Norman, T L; Ackerman, E S; Smith, T S; Gruen, T A; Yates, A J; Blaha, J D; Kish, V L

2006-02-01

Cementless total hip femoral components rely on press-fit for initial stability and bone healing and remodeling for secondary fixation. However, the determinants of satisfactory press-fit are not well understood. In previous studies, human cortical bone loaded circumferentially to simulate press-fit exhibited viscoelastic, or time dependent, behavior. The effect of bone viscoelastic behavior on the initial stability of press-fit stems is not known. Therefore, in the current study, push-out loads of cylindrical stems press-fit into reamed cadaver diaphyseal femoral specimens were measured immediately after assembly and 24 h with stem-bone diametral interference and stem surface treatment as independent variables. It was hypothesized that stem-bone interference would result in a viscoelastic response of bone that would decrease push-out load thereby impairing initial press-fit stability. Results showed that push-out load significantly decreased over a 24 h period due to bone viscoelasticity. It was also found that high and low push-out loads occurred at relatively small amounts of stem-bone interference, but a relationship between stem-bone interference and push-out load could not be determined due to variability among specimens. On the basis of this model, it was concluded that press-fit fixation can occur at relatively low levels of diametral interference and that stem-bone interference elicits viscoelastic response that reduces stem stability over time. From a clinical perspective, these results suggest that there could be large variations in initial press-fit fixation among patients.

Using Layer-by-Layer Coating and Nanocomposite Technologies to Improve the Barrier Properties of Polymeric Materials

NASA Astrophysics Data System (ADS)

Soltani, Iman

Means for improving barrier properties of polymers against gases, particularly for promoting their applications as packaging materials, are divided into surface coating and embedding nanoparticles in the bulk of the polymeric membranes. In this research, we mainly investigated improvement in barrier properties of polymers against oxygen and carbon dioxide, through layer-by-layer (LBL) coating and bulk nanocomposite methods. Initially, we studied the morphology of layer-by-layer assemblies comprising alternating layers of polyelectrolyte (PE) and natural montmorillonite (MMT) platelets, where polyethyleneterephthalate ionomer was used as our proposed alternative PE, to be compared with already examined polyethyleneimine. For both investigated PEs, while microscopic images showed the formation of tortuous networks of galleries between subsequent layers of oriented clay platelets parallel to the substrate surface, x-ray diffractometry (XRD) traces pointed to the intercalation of PE layers between clay platelets. As a confirmation of forming tortuous networks between oriented and high aspect ratio clay platelets to increase the path length of diffusing gas species dramatically, LBL-coated polystyrene-based membranes demonstrated pronounced decreases in permeability of oxygen and carbon dioxide (e.g. about the scale of 500 times decrease in permeability, with only five cycles of bilayer deposition). Before LBL deposition, the surface of the hydrophobic polymeric substrate was pretreated with oxygen plasma to improve its interaction with the coating. In the next study, previously LBL-coated samples were melt pressed in a cyclic manner to embed and to crush the coating inside the polystyrene-based matrix, aiming the exfoliated polymer-clay nanocomposites. The morphological investigations by transmission electron microscopy (TEM) revealed the tortuous internal structure of crushed LBL assemblies' portions, mainly comprising swollen intercalated stacks of clay, as well as flocculated exfoliated tactoids of a few clay platelets, down to about 2nm thickness. Moreover, XRD traces confirmed this increase in intercalation and exfoliation of clay platelets. Following ahead, dynamic mechanical thermal analysis (DMA) revealed significant increases in the storage and loss moduli values for our PNCs over those of pristine polystyrenebased matrix, hypothesizing the occurrence of substantial interactions between clay and the polymeric matrix, induced by intervening effect of PE interlayers. Also, permeation experiments showed noticeable improvement in gas barrier properties of processed PNCs. Considering the low content of clay particles and their limited level of global dispersions throughout the matrix, it may theorize the significant efficiency of high aspect ratio and tortuous LBL assemblies portions, oriented (induced by cycling pressing into thin films) perpendicular to the permeants' path routes. Thus, it might act almost as scavenging hubs against transport of diffusing gases. Finally, using PVAc, as the matrix, with this novel two-step approach of preparing PNCs, showed relatively higher clay content, when prepared with similar coating conditions. Also, DMA and permeation experiments pointed to significant improvements in mechanical and gas barrier properties of the PNCs, prepared by only 25 times melt pressing steps. Additionally, XRD traces postulated occurrence of noticeable irregularities in the interdistance of clay platelets. So, it is conjectured that semi-hydrophilic PVAc matrix promotes stronger interactions with clay particles, compared with those of polystyrene-based PNCs. However, moderate global dispersion of clay throughout the matrix points to the insufficient efficiency of repetitive melt pressing procedure to apply intensive enough stresses on samples, in order to overcome internal cohesion in LBL assemblies, which established initial intercalation and exfoliation in the otherwise aggregately clustered natural clay platelets. In addition, it is postulated that possibly occurring slight thermal degradations induce adverse results on the dispersion level and aforementioned properties of PNCs, processed over extended times.

The Quest for Community in a National Republic: A Bicentennial Reappraisal. Final Report and Keynote Address, Virginia Assembly (Richmond, Virginia, April 8-10, 1988).

ERIC Educational Resources Information Center

Virginia Univ., Charlottesville. Center for Public Service.

This Virginia Assembly document focuses on the concept of community as defined by the Founding Fathers of the U.S. Constitution. The final report identifies and discusses problems related to the modern quest for community in a national republic. Recommendations by the Assembly are offered in eight areas: (1) civic responsibility; (2) corporate…

Miniature multicontact connectors

NASA Technical Reports Server (NTRS)

Holden, D. G.; Robelen, D. B.

1970-01-01

Connectors, which have 4 to 9 contacts, are engaged through a hole with a minimum diameter of 0.030 inch. They are constructed of concentric brass and nylon components assembled with a light, firm press-fit. They withstand several thousand mating cycles and each outer contact has a one ampere capacity.

Let Freedom Run.

ERIC Educational Resources Information Center

Knopes, Carol; Hines, Judy

2002-01-01

Considers how the post 9/11 balancing act between stopping terrorism and protecting civil liberties shows how closely the rights of religion, speech, press, assembly and petition are intertwined. Examines the First Amendment in America's schools at the turn of the century. Presents a holistic approach to the First Amendment. (SG)

FILMING OF 'CONTACT' AT LC39 PRESS SITE SEEN FROM VAB ROOF

NASA Technical Reports Server (NTRS)

1997-01-01

The filming of scenes for the movie 'Contact' by Warner Bros.' cast and crew at Kennedy Space Center's Launch Complex 39 Press Site on January 29 is captured by cameras on the roof of the Vehicle Assembly Building. The screenplay for 'Contact' is based on the best-selling novel by the late astronomer Carl Sagan. The cast includes Jodie Foster, Matthew McConaughey, John Hurt, James Woods, Tom Skerritt, David Morse, William Fichtner, Rob Lowe and Angela Bassett. Described by Warner Bros. as a science fiction drama, 'Contact' will depict humankind's first encounter with evidence of extraterrestrial life.

FILMING OF 'CONTACT' AT LC39 PRESS SITE WITH VAB IN BACKGROUND

NASA Technical Reports Server (NTRS)

1997-01-01

With the Vehicle Assembly Building looming in the background, Warner Bros.' cast and crew are filming scenes for the movie 'Contact' at Kennedy Space Center's Launch Complex 39 Press Site on January 29. The screenplay for 'Contact' is based on the best- selling novel by the late astronomer Carl Sagan. The cast includes Jodie Foster, Matthew McConaughey, John Hurt, James Woods, Tom Skerritt, David Morse, William Fichtner, Rob Lowe and Angela Bassett. Described by Warner Bros. as a science fiction drama, 'Contact' will depict humankind's first encounter with evidence of extraterrestrial life.

FILMING OF 'CONTACT' AT LC39 PRESS SITE WITH VAB IN BACKGROUND

NASA Technical Reports Server (NTRS)

1997-01-01

With the Vehicle Assembly Building looming in the background, Warner Bros.' cast and crew are filming scenes for the movie 'Contact' at Kennedy Space Center's Launch Complex 39 Press Site on January 30. The screenplay for 'Contact' is based on the best- selling novel by the late astronomer Carl Sagan. The cast includes Jodie Foster, Matthew McConaughey, John Hurt, James Woods, Tom Skerritt, David Morse, William Fichtner, Rob Lowe and Angela Bassett. Described by Warner Bros. as a science fiction drama, 'Contact' will depict humankind's first encounter with evidence of extraterrestrial life.

Approaches to self-assembly of colloidal monolayers: A guide for nanotechnologists.

PubMed

Lotito, Valeria; Zambelli, Tomaso

2017-08-01

Self-assembly of quasi-spherical colloidal particles in two-dimensional (2D) arrangements is essential for a wide range of applications from optoelectronics to surface engineering, from chemical and biological sensing to light harvesting and environmental remediation. Several self-assembly approaches have flourished throughout the years, with specific features in terms of complexity of the implementation, sensitivity to process parameters, characteristics of the final colloidal assembly. Selecting the proper method for a given application amidst the vast literature in this field can be a challenging task. In this review, we present an extensive classification and comparison of the different techniques adopted for 2D self-assembly in order to provide useful guidelines for scientists approaching this field. After an overview of the main applications of 2D colloidal assemblies, we describe the main mechanisms underlying their formation and introduce the mathematical tools commonly used to analyse their final morphology. Subsequently, we examine in detail each class of self-assembly techniques, with an explanation of the physical processes intervening in crystallization and a thorough investigation of the technical peculiarities of the different practical implementations. We point out the specific characteristics of the set-ups and apparatuses developed for self-assembly in terms of complexity, requirements, reproducibility, robustness, sensitivity to process parameters and morphology of the final colloidal pattern. Such an analysis will help the reader to individuate more easily the approach more suitable for a given application and will draw the attention towards the importance of the details of each implementation for the final results. Copyright © 2017 Elsevier B.V. All rights reserved.

Registry in a tube: multiplexed pools of retrievable parts for genetic design space exploration.

PubMed

Woodruff, Lauren B A; Gorochowski, Thomas E; Roehner, Nicholas; Mikkelsen, Tarjei S; Densmore, Douglas; Gordon, D Benjamin; Nicol, Robert; Voigt, Christopher A

2017-02-17

Genetic designs can consist of dozens of genes and hundreds of genetic parts. After evaluating a design, it is desirable to implement changes without the cost and burden of starting the construction process from scratch. Here, we report a two-step process where a large design space is divided into deep pools of composite parts, from which individuals are retrieved and assembled to build a final construct. The pools are built via multiplexed assembly and sequenced using next-generation sequencing. Each pool consists of ∼20 Mb of up to 5000 unique and sequence-verified composite parts that are barcoded for retrieval by PCR. This approach is applied to a 16-gene nitrogen fixation pathway, which is broken into pools containing a total of 55 848 composite parts (71.0 Mb). The pools encompass an enormous design space (1043 possible 23 kb constructs), from which an algorithm-guided 192-member 4.5 Mb library is built. Next, all 1030 possible genetic circuits based on 10 repressors (NOR/NOT gates) are encoded in pools where each repressor is fused to all permutations of input promoters. These demonstrate that multiplexing can be applied to encompass entire design spaces from which individuals can be accessed and evaluated. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

BioPartsBuilder: a synthetic biology tool for combinatorial assembly of biological parts.

PubMed

Yang, Kun; Stracquadanio, Giovanni; Luo, Jingchuan; Boeke, Jef D; Bader, Joel S

2016-03-15

Combinatorial assembly of DNA elements is an efficient method for building large-scale synthetic pathways from standardized, reusable components. These methods are particularly useful because they enable assembly of multiple DNA fragments in one reaction, at the cost of requiring that each fragment satisfies design constraints. We developed BioPartsBuilder as a biologist-friendly web tool to design biological parts that are compatible with DNA combinatorial assembly methods, such as Golden Gate and related methods. It retrieves biological sequences, enforces compliance with assembly design standards and provides a fabrication plan for each fragment. BioPartsBuilder is accessible at http://public.biopartsbuilder.org and an Amazon Web Services image is available from the AWS Market Place (AMI ID: ami-508acf38). Source code is released under the MIT license, and available for download at https://github.com/baderzone/biopartsbuilder joel.bader@jhu.edu Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press.

KSC-2011-3419

NASA Image and Video Library

2011-05-09

CAPE CANAVERAL, Fla. -- In the Press Site auditorium at NASA's Kennedy Space Center in Florida, Space Shuttle Program Launch Integration Manager Mike Moses briefs media about the launch status of space shuttle Endeavour's STS-134 mission and announces a new launch date. Technicians replaced and tested the aft load control assembly-2 (ALCA-2) and wiring located in Endeavour's aft avionics bay 5. ALCA-2 distributes power to nine shuttle systems and is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt. Launch now is scheduled for May 16 at 8:56 a.m. EDT. Endeavour and its crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the station. This will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

KSC-2011-3421

NASA Image and Video Library

2011-05-09

CAPE CANAVERAL, Fla. -- In the Press Site auditorium at NASA's Kennedy Space Center in Florida, Shuttle Launch Director Mike Leinbach briefs media about the launch status of space shuttle Endeavour's STS-134 mission and announces a new launch date. Technicians replaced and tested the aft load control assembly-2 (ALCA-2) and wiring located in Endeavour's aft avionics bay 5. ALCA-2 distributes power to nine shuttle systems and is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt. Launch now is scheduled for May 16 at 8:56 a.m. EDT. Endeavour and its crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the station. This will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

MetaPathways v2.5: quantitative functional, taxonomic and usability improvements.

PubMed

Konwar, Kishori M; Hanson, Niels W; Bhatia, Maya P; Kim, Dongjae; Wu, Shang-Ju; Hahn, Aria S; Morgan-Lang, Connor; Cheung, Hiu Kan; Hallam, Steven J

2015-10-15

Next-generation sequencing is producing vast amounts of sequence information from natural and engineered ecosystems. Although this data deluge has an enormous potential to transform our lives, knowledge creation and translation need software applications that scale with increasing data processing and analysis requirements. Here, we present improvements to MetaPathways, an annotation and analysis pipeline for environmental sequence information that expedites this transformation. We specifically address pathway prediction hazards through integration of a weighted taxonomic distance and enable quantitative comparison of assembled annotations through a normalized read-mapping measure. Additionally, we improve LAST homology searches through BLAST-equivalent E-values and output formats that are natively compatible with prevailing software applications. Finally, an updated graphical user interface allows for keyword annotation query and projection onto user-defined functional gene hierarchies, including the Carbohydrate-Active Enzyme database. MetaPathways v2.5 is available on GitHub: http://github.com/hallamlab/metapathways2. shallam@mail.ubc.ca Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press.

Parameters, U.S. Army War College Quarterly. Volume 23, Number 2, Summer 1993

DTIC Science & Technology

1993-01-01

conventional combat. Drug Trafficking In many cases, Sendero has allied itself with the narcoiraffickers to gain needed financial support. Peruvian...and sometimes identical) heterosexual shenanigans could be assembled from the popular. soft-porn press. The other problem is that just as there is

4 CFR 83.10 - First Amendment rights.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 4 Accounts 1 2011-01-01 2011-01-01 false First Amendment rights. 83.10 Section 83.10 Accounts GOVERNMENT ACCOUNTABILITY OFFICE RECORDS PRIVACY PROCEDURES FOR PERSONNEL RECORDS § 83.10 First Amendment... beliefs, freedom of speech and the press, and freedom to assemble and to petition the Government. ...

4 CFR 83.10 - First Amendment rights.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 4 Accounts 1 2012-01-01 2012-01-01 false First Amendment rights. 83.10 Section 83.10 Accounts GOVERNMENT ACCOUNTABILITY OFFICE RECORDS PRIVACY PROCEDURES FOR PERSONNEL RECORDS § 83.10 First Amendment... beliefs, freedom of speech and the press, and freedom to assemble and to petition the Government. ...

4 CFR 83.10 - First Amendment rights.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 4 Accounts 1 2013-01-01 2013-01-01 false First Amendment rights. 83.10 Section 83.10 Accounts GOVERNMENT ACCOUNTABILITY OFFICE RECORDS PRIVACY PROCEDURES FOR PERSONNEL RECORDS § 83.10 First Amendment... beliefs, freedom of speech and the press, and freedom to assemble and to petition the Government. ...

4 CFR 83.10 - First Amendment rights.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 4 Accounts 1 2010-01-01 2010-01-01 false First Amendment rights. 83.10 Section 83.10 Accounts GOVERNMENT ACCOUNTABILITY OFFICE RECORDS PRIVACY PROCEDURES FOR PERSONNEL RECORDS § 83.10 First Amendment... beliefs, freedom of speech and the press, and freedom to assemble and to petition the Government. ...

Statistical Aspects of Reliability, Maintainability, and Availability.

DTIC Science & Technology

1987-10-01

A total of 33 research reports were issued, and 35 papers were published in scientific journals or are in press. Research topics included optimal assembly of systems, multistate system theory , testing whether new is better than used nonparameter survival function estimation measuring information in censored models, generalizations of total positively and

What Really Works with Exceptional Learners

ERIC Educational Resources Information Center

Murawski, Wendy W., Ed.; Scott, Kathy Lynn, Ed.

2017-01-01

Today's teachers, especially the many who work with students with special needs, are pressed to know a little something about everything. Expertise is needed in different content and pedagogical areas, but knowledge of different types of disabilities is required as well. Wendy Murawski and Kathy Lynn Scott have assembled another reader-friendly…

High-temperature, high-pressure bonding of nested tubular metallic components

DOEpatents

Quinby, T.C.

A tool is described for effecting high-temperature, high-compression bonding between the confronting faces of nested, tubular, metallic components. In a typical application, the tool is used to produce tubular target assemblies for irradiation in nuclear reactors or particle accelerators. The target assembly comprising a uranum foil and an aluninum-alloy substrate. The tool is composed of graphite. It comprises a tubular restraining member in which a mechanically expandable tubular core is mounted to form an annulus. The components to be bonded are mounted in nested relation in the annulus. The expandable core is formed of individually movable, axially elongated segments whose outer faces cooperatively define a cylindrical pressing surface and whose inner faces cooperatively define two opposed, inwardly tapered, axial bores. Tapered rams extend into the bores. The loaded tool is mounted in a conventional hot-press provided with evacuation means, heaters for maintaining its interior at bonding temperature, and hydraulic cylinders for maintaining a selected inwardly directed pressure on the tapered rams. With the hot-press evacuated and the loaded tool at the desired temperature, the cylinders are actuated to apply the selected pressure to the rams. The rams in turn expand the segmented core to maintain the nested components in compression against the restraining member. These conditions are maintained until the confronting faces of the nested components are joined in a continuous, uniform bond characterized by high thermal conductivity.

Drug Target Validation Methods in Malaria - Protein Interference Assay (PIA) as a Tool for Highly Specific Drug Target Validation.

PubMed

Meissner, Kamila A; Lunev, Sergey; Wang, Yuan-Ze; Linzke, Marleen; de Assis Batista, Fernando; Wrenger, Carsten; Groves, Matthew R

2017-01-01

The validation of drug targets in malaria and other human diseases remains a highly difficult and laborious process. In the vast majority of cases, highly specific small molecule tools to inhibit a proteins function in vivo are simply not available. Additionally, the use of genetic tools in the analysis of malarial pathways is challenging. These issues result in difficulties in specifically modulating a hypothetical drug target's function in vivo. The current "toolbox" of various methods and techniques to identify a protein's function in vivo remains very limited and there is a pressing need for expansion. New approaches are urgently required to support target validation in the drug discovery process. Oligomerisation is the natural assembly of multiple copies of a single protein into one object and this self-assembly is present in more than half of all protein structures. Thus, oligomerisation plays a central role in the generation of functional biomolecules. A key feature of oligomerisation is that the oligomeric interfaces between the individual parts of the final assembly are highly specific. However, these interfaces have not yet been systematically explored or exploited to dissect biochemical pathways in vivo. This mini review will describe the current state of the antimalarial toolset as well as the potentially druggable malarial pathways. A specific focus is drawn to the initial efforts to exploit oligomerisation surfaces in drug target validation. As alternative to the conventional methods, Protein Interference Assay (PIA) can be used for specific distortion of the target protein function and pathway assessment in vivo. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

32 CFR 644.71 - Final title assembly.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 4 2013-07-01 2013-07-01 false Final title assembly. 644.71 Section 644.71 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL... deeds and related papers in acquisitions for the Strategic Petroleum Reserve Program of the Department...

32 CFR 644.71 - Final title assembly.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 4 2011-07-01 2011-07-01 false Final title assembly. 644.71 Section 644.71 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL... deeds and related papers in acquisitions for the Strategic Petroleum Reserve Program of the Department...

32 CFR 644.71 - Final Title Assembly.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 4 2010-07-01 2010-07-01 true Final Title Assembly. 644.71 Section 644.71 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL... deeds and related papers in acquisitions for the Strategic Petroleum Reserve Program of the Department...

32 CFR 644.71 - Final title assembly.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 4 2012-07-01 2011-07-01 true Final title assembly. 644.71 Section 644.71 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL... deeds and related papers in acquisitions for the Strategic Petroleum Reserve Program of the Department...

32 CFR 644.71 - Final title assembly.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 4 2014-07-01 2013-07-01 true Final title assembly. 644.71 Section 644.71 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY (CONTINUED) REAL PROPERTY REAL... deeds and related papers in acquisitions for the Strategic Petroleum Reserve Program of the Department...

KSC-2011-5256

NASA Image and Video Library

2011-07-08

CAPE CANAVERAL, Fla. -- Media from around the globe gather at the NASA News Center at NASA Kennedy Space Center's Press Site in Florida to photograph and cover the prelaunch activities and lift off of space shuttle Atlantis on its STS-135 mission to the International Space Station. Satellite news trucks, trailers and automobiles can be seen in the parking lot with the massive Vehicle Assembly Building towering above. Atlantis began its final flight, with Commander Chris Ferguson, Pilot Doug Hurley and Mission Specialists Sandy Magnus and Rex Walheim on board, at 11:29 a.m. EDT July 8 to deliver the Raffaello multi-purpose logistics module packed with supplies and spare parts to the station. Also in Atlantis' payload bay is the Robotic Refueling Mission experiment that will investigate the potential for robotically refueling existing satellites in orbit. In addition, Atlantis will return with a failed ammonia pump module to help NASA better understand the failure mechanism and improve pump designs for future systems. STS-135 is the 33rd flight of Atlantis, the 37th shuttle mission to the space station, and the 135th and final mission of NASA's Space Shuttle Program. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-5234

NASA Image and Video Library

2011-07-08

CAPE CANAVERAL, Fla. -- Media from around the globe gather on the grounds of the Press Site at NASA's Kennedy Space Center in Florida to photograph and cover the prelaunch activities and lift off of space shuttle Atlantis on its STS-135 mission to the International Space Station. Dozens of satellite news vehicles can be seen in the parking lot while the massive Vehicle Assembly Building towers above in the background. Atlantis began its final flight, with Commander Chris Ferguson, Pilot Doug Hurley and Mission Specialists Sandy Magnus and Rex Walheim on board, at 11:29 a.m. EDT July 8 to deliver the Raffaello multi-purpose logistics module packed with supplies and spare parts to the station. Also in Atlantis' payload bay is the Robotic Refueling Mission experiment that will investigate the potential for robotically refueling existing satellites in orbit. In addition, Atlantis will return with a failed ammonia pump module to help NASA better understand the failure mechanism and improve pump designs for future systems. STS-135 is the 33rd flight of Atlantis, the 37th shuttle mission to the space station, and the 135th and final mission of NASA's Space Shuttle Program. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-5237

NASA Image and Video Library

2011-07-08

CAPE CANAVERAL, Fla. -- Media from around the globe gather on the grounds of the Press Site at NASA's Kennedy Space Center in Florida to photograph and cover the prelaunch activities and lift off of space shuttle Atlantis on its STS-135 mission to the International Space Station. Dozens of satellite news vehicles can be seen in the parking lot with the massive Vehicle Assembly Building towering above. Atlantis began its final flight, with Commander Chris Ferguson, Pilot Doug Hurley and Mission Specialists Sandy Magnus and Rex Walheim on board, at 11:29 a.m. EDT July 8 to deliver the Raffaello multi-purpose logistics module packed with supplies and spare parts to the station. Also in Atlantis' payload bay is the Robotic Refueling Mission experiment that will investigate the potential for robotically refueling existing satellites in orbit. In addition, Atlantis will return with a failed ammonia pump module to help NASA better understand the failure mechanism and improve pump designs for future systems. STS-135 is the 33rd flight of Atlantis, the 37th shuttle mission to the space station, and the 135th and final mission of NASA's Space Shuttle Program. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Jim Grossmann

KSC-2011-5235

NASA Image and Video Library

2011-07-08

CAPE CANAVERAL, Fla. -- Media from around the globe gather on the grounds of the Press Site at NASA's Kennedy Space Center in Florida to photograph and cover the prelaunch activities and lift off of space shuttle Atlantis on its STS-135 mission to the International Space Station. Dozens of satellite news vehicles can be seen in the parking lot while the massive Vehicle Assembly Building towers above in the background. Atlantis began its final flight, with Commander Chris Ferguson, Pilot Doug Hurley and Mission Specialists Sandy Magnus and Rex Walheim on board, at 11:29 a.m. EDT July 8 to deliver the Raffaello multi-purpose logistics module packed with supplies and spare parts to the station. Also in Atlantis' payload bay is the Robotic Refueling Mission experiment that will investigate the potential for robotically refueling existing satellites in orbit. In addition, Atlantis will return with a failed ammonia pump module to help NASA better understand the failure mechanism and improve pump designs for future systems. STS-135 is the 33rd flight of Atlantis, the 37th shuttle mission to the space station, and the 135th and final mission of NASA's Space Shuttle Program. For more information, visit www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts135/index.html. Photo credit: NASA/Jim Grossmann

KSC-82PC-0331

NASA Image and Video Library

1982-03-22

The Complex 39 press site buzzes with activity during the final countdown to the launch of the third space shuttle flight. More than 2,000 news media representatives from around the world crowded onto the press site this morning to watch the successful 11 a.m. launch.

The SSU processome interactome in Saccharomyces cerevisiae reveals novel protein subcomplexes.

PubMed

Vincent, Nicholas G; Charette, J Michael; Baserga, Susan J

2018-01-01

Ribosome assembly is an evolutionarily conserved and energy intensive process required for cellular growth, proliferation, and maintenance. In yeast, assembly of the small ribosomal subunit (SSU) requires approximately 75 assembly factors that act in coordination to form the SSU processome, a 6 MDa ribonucleoprotein complex. The SSU processome is required for processing, modifying, and folding the preribosomal RNA (rRNA) to prepare it for incorporation into the mature SSU. Although the protein composition of the SSU processome has been known for some time, the interaction network of the proteins required for its assembly has remained poorly defined. Here, we have used a semi-high-throughput yeast two-hybrid (Y2H) assay and coimmunoprecipitation validation method to produce a high-confidence interactome of SSU processome assembly factors (SPAFs), providing essential insight into SSU assembly and ribosome biogenesis. Further, we used glycerol density-gradient sedimentation to reveal the presence of protein subcomplexes that have not previously been observed. Our work not only provides essential insight into SSU assembly and ribosome biogenesis, but also serves as an important resource for future investigations into how defects in biogenesis and assembly cause congenital disorders of ribosomes known as ribosomopathies. © 2018 Vincent et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

Final Approval of California Air Plan Revision; Antelope Valley Air Quality Management District; VOCs From Motor Vehicle Assembly Coating Operations

EPA Pesticide Factsheets

EPA is taking final action to approve a revision to the Antelope Valley Air Quality Management District (AVAQMD) portion of the California SIP concerning the emissions of volatile organic compounds (VOCs) from motor vehicle assembly coating operations.

F-35 Engine Quality Assurance Inspection

DTIC Science & Technology

2015-04-27

area nor protected from common FOD items. The F135 engine final assembly area had FOD signage at the two entry lobbies of the building; however...there were no FOD signage within the engine final assembly areas. Pratt & Whitney FOD procedures also did not prevent common FOD items from entering

Validation of an optical encoder during free weight resistance movements and analysis of bench press sticking point power during fatigue.

PubMed

Drinkwater, Eric J; Galna, Brook; McKenna, Michael J; Hunt, Patrick H; Pyne, David B

2007-05-01

During the concentric movement of the bench press, there is an initial high-power push after chest contact, immediately followed by a characteristic area of low power, the so-called "sticking region." During high-intensity lifting, a decline in power can result in a failed lift attempt. The purpose of this study was to determine the validity of an optical encoder to measure power and then employ this device to determine power changes during the initial acceleration and sticking region during fatiguing repeated bench press training. Twelve subjects performed a free weight bench press, a Smith Machine back squat, and a Smith Machine 40-kg bench press throw for power validation measures. All barbell movements were simultaneously monitored using cinematography and an optical encoder. Eccentric and concentric mean and peak power were calculated using time and position data derived from each method. Validity of power measures between the video (criterion) and optical encoder scores were evaluated by standard error of the estimate (SEE) and coefficient of variation (CV). Seven subjects then performed 4 sets of 6 free weight bench press repetitions progressively increasing from 85 to 95% of their 6 repetition maximum, with each repetition continually monitored by an optical encoder. The SEE for power ranged from 3.6 to 14.4 W (CV, 1.0-3.0%; correlation, 0.97-1.00). During the free weight bench press training, peak power declined by approximately 55% (p < 0.01) during the initial acceleration phase of the final 2 repetitions of the final set. Although decreases in power of the sticking point were significant (p < 0.01), as early as repetition 5 (-40%) they reached critically low levels in the final 2 repetitions (>-95%). In conclusion, the optical encoder provided valid measures of kinetics during free weight resistance training movements. The decline in power during the initial acceleration phase appears a factor in a failed lift attempt at the sticking point.

41 CFR 51-9.101-3 - Content of systems of records.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 41 Public Contracts and Property Management 1 2014-07-01 2014-07-01 false Content of systems of records. 51-9.101-3 Section 51-9.101-3 Public Contracts and Property Management Other Provisions Relating..., religious and political beliefs, freedom of speech, the press, assembly, and freedom to petition. ...

41 CFR 51-9.101-3 - Content of systems of records.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 41 Public Contracts and Property Management 1 2013-07-01 2013-07-01 false Content of systems of records. 51-9.101-3 Section 51-9.101-3 Public Contracts and Property Management Other Provisions Relating..., religious and political beliefs, freedom of speech, the press, assembly, and freedom to petition. ...

NRL Review - 2009

DTIC Science & Technology

2009-01-01

for a fundamental physical understanding of electronic properties . The Materials Processing Facility includes appa- ratuses for powder production by...situ. Facilities to process powder into bulk specimens by hot and cold isostatic pressing permit a variety of consolidation possibilities. The iso...Synthesis/ Property Measurement Facility has special emphasis on polymers, surface-film processing , and directed self-assembly. The Chemical Vapor

OAS :: Press Releases

Science.gov Websites

General Assembly Governance H Human Development Human Rights I Indigenous Peoples Integral Development Scholarships School of Governance Science and Technology Social Development Summits of the Americas Sustainable : 1 2 Next » S-022/18 May 25, 2018 FACT SHEET: OAS School of Governance S-021/18 May 24, 2018 FACT

Titanium dental copings prepared by a powder metallurgy method: a preliminary report.

PubMed

Eriksson, Mikael; Andersson, Matts; Carlström, Elis

2004-01-01

The purpose of this study was to determine if the Procera pressed-powder method can be used to fabricate titanium copings. Commercially pure titanium powder was used to prepare the copings. The powder was pressed onto an enlarged tooth preparation die of aluminum using cold isostatic pressing. The outer shape of the coping was formed using a Procera milling machine, and the copings were vacuum sintered. Titanium copings could be prepared using this method. The density of the sintered copings reached 97% to 99%+ of theoretic density, and the copings showed ductile behavior after sintering. Enlarging the tooth preparation die to compensate for the sintering shrinkage could optimize the final size of the copings. Ductile and dense titanium dental copings can be produced with powder-metal processing using cold isostatic pressing, followed by milling and sintering to final shape. The forming technique has, if properly optimized, a potential of becoming a more cost-efficient production method than spark erosion.

SynTrack: DNA Assembly Workflow Management (SynTrack) v2.0.1

DOE Office of Scientific and Technical Information (OSTI.GOV)

MENG, XIANWEI; SIMIRENKO, LISA

2016-12-01

SynTrack is a dynamic, workflow-driven data management system that tracks the DNA build process: Management of the hierarchical relationships of the DNA fragments; Monitoring of process tasks for the assembly of multiple DNA fragments into final constructs; Creations of vendor order forms with selectable building blocks. Organizing plate layouts barcodes for vendor/pcr/fusion/chewback/bioassay/glycerol/master plate maps (default/condensed); Creating or updating Pre-Assembly/Assembly process workflows with selected building blocks; Generating Echo pooling instructions based on plate maps; Tracking of building block orders, received and final assembled for delivering; Bulk updating of colony or PCR amplification information, fusion PCR and chewback results; Updating with QA/QCmore » outcome with .csv & .xlsx template files; Re-work assembly workflow enabled before and after sequencing validation; and Tracking of plate/well data changes and status updates and reporting of master plate status with QC outcomes.« less

Process development for automated solar cell and module production. Task 4: Automated array assembly

NASA Technical Reports Server (NTRS)

Hagerty, J. J.

1981-01-01

The Unimate robot was programmed for the final 35 cell pattern to be used in the fabrication of the deliverable modules. Mechanical construction of the Automated Lamination Station and Final Assembly Station were completed on schedule. All final wiring and interconnect cables were also completed and the first operational testing began. The final controlling program was written. A local fabricator was contracted to produce the glass reinforced concrete panels to be used for testing and deliverables. A video tape showing all three stations in operation was produced.

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 Soyuz commander Aleksandr Skvortsov is seen in quarantine, behind glass, during the final press conference be Monday, March 24, 2014, at the Cosmonaut Hotel in Baikonur, Kazakhstan. The mission to the International Space Station is set to launch March 26 from the Baikonur Cosmodrome. Photo Credit: (NASA/Joel Kowsky)

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 flight engineer Oleg Artemyev is seen in quarantine, behind glass, during the final press conference be Monday, March 24, 2014, at the Cosmonaut Hotel in Baikonur, Kazakhstan. The mission to the International Space Station is set to launch March 26 from the Baikonur Cosmodrome. Photo Credit: (NASA/Joel Kowsky)

LightAssembler: fast and memory-efficient assembly algorithm for high-throughput sequencing reads.

PubMed

El-Metwally, Sara; Zakaria, Magdi; Hamza, Taher

2016-11-01

The deluge of current sequenced data has exceeded Moore's Law, more than doubling every 2 years since the next-generation sequencing (NGS) technologies were invented. Accordingly, we will able to generate more and more data with high speed at fixed cost, but lack the computational resources to store, process and analyze it. With error prone high throughput NGS reads and genomic repeats, the assembly graph contains massive amount of redundant nodes and branching edges. Most assembly pipelines require this large graph to reside in memory to start their workflows, which is intractable for mammalian genomes. Resource-efficient genome assemblers combine both the power of advanced computing techniques and innovative data structures to encode the assembly graph efficiently in a computer memory. LightAssembler is a lightweight assembly algorithm designed to be executed on a desktop machine. It uses a pair of cache oblivious Bloom filters, one holding a uniform sample of [Formula: see text]-spaced sequenced [Formula: see text]-mers and the other holding [Formula: see text]-mers classified as likely correct, using a simple statistical test. LightAssembler contains a light implementation of the graph traversal and simplification modules that achieves comparable assembly accuracy and contiguity to other competing tools. Our method reduces the memory usage by [Formula: see text] compared to the resource-efficient assemblers using benchmark datasets from GAGE and Assemblathon projects. While LightAssembler can be considered as a gap-based sequence assembler, different gap sizes result in an almost constant assembly size and genome coverage. https://github.com/SaraEl-Metwally/LightAssembler CONTACT: sarah_almetwally4@mans.edu.egSupplementary information: Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Colloidosome like structures: self-assembly of silica microrods

DOE PAGES

Datskos, P.; Polizos, G.; Bhandari, M.; ...

2016-03-07

Self-assembly of one-dimensional structures is attracting a great deal of interest because assembled structures can provide better properties compared to individual building blocks. We demonstrate silica microrod self-assembly by exploiting Pickering emulsion based strategy. Micron-sized silica rods were synthesized employing previously reported methods based on polyvinylpyrrolidone/ pentanol emulsion droplets. Moreover, rods self-assembled to make structures in the range of z10 40 mm. Smooth rods assembled better than segmented rods. Finally, the assembled structures were bonded by weak van der Waals forces.

Improvements to highway guardrail assemblies : final report 14-1TIRE.

DOT National Transportation Integrated Search

2016-05-01

Highway guardrail assemblies play an important role in enhancing the safety of motorists. Guardrail assemblies : contain three main components: (1) galvanized steel guardrail, (2) posts, and (3) blockouts. The purpose of the : blockout is to increase...

Single haplotype assembly of the human genome from a hydatidiform mole.

PubMed

Steinberg, Karyn Meltz; Schneider, Valerie A; Graves-Lindsay, Tina A; Fulton, Robert S; Agarwala, Richa; Huddleston, John; Shiryev, Sergey A; Morgulis, Aleksandr; Surti, Urvashi; Warren, Wesley C; Church, Deanna M; Eichler, Evan E; Wilson, Richard K

2014-12-01

A complete reference assembly is essential for accurately interpreting individual genomes and associating variation with phenotypes. While the current human reference genome sequence is of very high quality, gaps and misassemblies remain due to biological and technical complexities. Large repetitive sequences and complex allelic diversity are the two main drivers of assembly error. Although increasing the length of sequence reads and library fragments can improve assembly, even the longest available reads do not resolve all regions. In order to overcome the issue of allelic diversity, we used genomic DNA from an essentially haploid hydatidiform mole, CHM1. We utilized several resources from this DNA including a set of end-sequenced and indexed BAC clones and 100× Illumina whole-genome shotgun (WGS) sequence coverage. We used the WGS sequence and the GRCh37 reference assembly to create an assembly of the CHM1 genome. We subsequently incorporated 382 finished BAC clone sequences to generate a draft assembly, CHM1_1.1 (NCBI AssemblyDB GCA_000306695.2). Analysis of gene, repetitive element, and segmental duplication content show this assembly to be of excellent quality and contiguity. However, comparison to assembly-independent resources, such as BAC clone end sequences and PacBio long reads, indicate misassembled regions. Most of these regions are enriched for structural variation and segmental duplication, and can be resolved in the future. This publicly available assembly will be integrated into the Genome Reference Consortium curation framework for further improvement, with the ultimate goal being a completely finished gap-free assembly. © 2014 Steinberg et al.; Published by Cold Spring Harbor Laboratory Press.

Development of Kawai-type multianvil technology using nano-polycrystalline diamond anvils

NASA Astrophysics Data System (ADS)

Irifune, T.; Kunimoto, T.

2016-12-01

Nano-polycrystalline diamond (NPD) developed at GRC, Ehime Univ., is known to be much harder than conventional sintered polycrystalline diamond (SD), and is potentially important as material for anvils for Kawai-type (6-8 type) multianvil apparatus (KMA), as well as for diamond anvil cell. We synthesized NPD rods with about 8 mm in both diameter and length using a 6000-ton press KMA (BOTCHAN-6000), which are cut by pulsed-laser to form cubes with 6 mm edge length and tested them as anvils for KMA. In situ X-ray observations were made to evaluate the produced pressures and sample images using the "6-6-8 assembly". A combination of semi-fired pyrophyllite gaskets and alumina ceramics pressure medium optimized for the NPD anvils with a truncation (TEL) of 1.0 mm yielded pressures up to 88 GPa at a press load of only 3.4 MN, which is nearly 60% higher than the pressure (56 GPa) reached using SD anvils with the identical cell assembly at the same press load. Moreover, the high X-ray transparency of NPD has enabled us to clearly see the sample image via the anvils, allowing diffraction measurements and observations of the sample shape even if the anvil gap becomes very small under such very high pressures. The use of NPD anvils should lead to new technology for mineral physics studies under the conditions of the Earth's lowermost mantle and possibly those of the core without scarifying the advantages of KMA over DAC.

High-temperature, high-pressure bonding of nested tubular metallic components

DOEpatents

Quinby, Thomas C.

1980-01-01

This invention is a tool for effecting high-temperature, high-compression bonding between the confronting faces of nested, tubular, metallic components. In a typical application, the tool is used to produce tubular target assemblies for irradiation in nuclear reactors or particle accelerators, the target assembly comprising a uranium foil and an aluminum-alloy substrate. The tool preferably is composed throughout of graphite. It comprises a tubular restraining member in which a mechanically expandable tubular core is mounted to form an annulus with the member. The components to be bonded are mounted in nested relation in the annulus. The expandable core is formed of individually movable, axially elongated segments whose outer faces cooperatively define a cylindrical pressing surface and whose inner faces cooperatively define two opposed, inwardly tapered, axial bores. Tapered rams extend respectively into the bores. The loaded tool is mounted in a conventional hot-press provided with evacuation means, heaters for maintaining its interior at bonding temperature, and hydraulic cylinders for maintaining a selected inwardly directed pressure on the tapered rams. With the hot-press evacuated and the loaded tool at the desired temperature, the cylinders are actuated to apply the selected pressure to the rams. The rams in turn expand the segmented core to maintain the nested components in compression against the restraining member. These conditions are maintained until the confronting faces of the nested components are joined in a continuous, uniform bond characterized by high thermal conductivity.

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 flight engineer Steve Swanson of NASA is seen in quarantine, behind glass, during the final press conference be Monday, March 24, 2014, at the Cosmonaut Hotel in Baikonur, Kazakhstan. The mission to the International Space Station is set to launch March 26 from the Baikonur Cosmodrome. Photo Credit: (NASA/Bill Ingalls)

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 flight engineer Steve Swanson of NASA is seen in quarantine, behind glass, during the final press conference be Monday, March 24, 2014, at the Cosmonaut Hotel in Baikonur, Kazakhstan. The mission to the International Space Station is set to launch March 26 from the Baikonur Cosmodrome. Photo Credit: (NASA/Joel Kowsky)

Flexural resistance of heat-pressed and CAD-CAM lithium disilicate with different translucencies.

PubMed

Fabian Fonzar, Riccardo; Carrabba, Michele; Sedda, Maurizio; Ferrari, Marco; Goracci, Cecilia; Vichi, Alessandro

2017-01-01

To compare flexural strength of CAD-CAM and heat-pressed lithium disilicate. For Pressed specimens (Group A), acrylate polymer blocks were cut with a saw in bars shape. Sprueing, investing and preheating procedures were carried out following manufacturer's instructions. IPS e.max Press ingots (Ivoclar-Vivadent) were divided into subgroups (n=15) according to translucency: A.1=HT-A3; A.2=MT-A3; A.3=LT-A3; A.4=MO2. Ingots were then pressed following manufacturer's instructions. For CAD-CAM specimens (Group B) blocks of IPS e.max CAD (Ivoclar-Vivadent) were divided into subgroups: B.1=HT-A3; B.2=MT-A3; B.3=LT-A3; B.4=MO2. Specimens (n=15) were obtained by cutting the blocks with a saw. Final crystallization was performed following manufacturer's instructions. Both Press and CAD specimens were polished and finished with silica carbide papers of increasing grit. Final dimensions of the specimens were 4.0±0.2mm, 1.2±0.2mm, and 16.0±0.2mm. Specimens were tested using a three-point bending test. Flexural strength, Weibull modulus, and Weibull characteristic strength were calculated. Flexural strength data were statistically analyzed. The overall means of Press and CAD specimens did not differ significantly. Within the Press group different translucencies were found to have similar flexural strength. Within the CAD group, statistically significant differences emerged among the tested translucencies (p<0.001). Specifically, MT had significantly higher flexural strength than HT and MO. Also, LT exhibited significantly higher flexural strength than MO. The choice between IPS e.max Press and IPS e.max CAD formulations can be based on different criteria than flexural resistance. Within each formulation, for IPS e.max Press translucency does not affect the flexural strength while for IPS e.max CAD it is an influential factor. Copyright © 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Enhancement in transport critical current density of ex situ PIT Ag/(Ba, K)Fe2As2 tapes achieved by applying a combined process of flat rolling and uniaxial pressing

NASA Astrophysics Data System (ADS)

Togano, Kazumasa; Gao, Zhaoshun; Matsumoto, Akiyoshi; Kumakura, Hiroaki

2013-11-01

We report that the transport critical current density Jc of ex situ powder-in-tube (PIT) processed (Ba, K)Fe2As2 (Ba-122) tapes can be significantly enhanced by applying uniaxial cold pressing at the final stage of deformation. The tapes were prepared by packing high quality precursor powder into a Ag tube, cycles of rolling and intermediate annealing, and pressing followed by the final heat treatment for sintering. The Jc values in applied magnetic fields were increased by almost one order of magnitude compared to the tapes processed without pressing, exceeding 104 A cm-2 at 4.2 K. We achieved the highest Jc (at 4.2 K and 10 T) of 2.1×104 A cm-2 among PIT-processed Fe-based wires and tapes reported so far. The Jc-H curves measured at higher temperatures maintain small field dependence up to around 20 K, suggesting that these tapes are promising for applications at higher temperatures as well as at liquid helium temperature. The microstructure investigations reveal that there are two possible causes of the large Jc enhancement by pressing: one is densification and the other is the change of crack structure. Optimization of processing parameters such as the reduction ratio of rolling and pressing is expected to yield further Jc enhancement.

Physical properties of a resin system for filling the inter-space in the ITER TF coil casing

NASA Astrophysics Data System (ADS)

Evans, D.; Baynahm, E.; Canfer, S.; Foussat, A.

2014-01-01

Each of the eighteen ITER Toroidal Field (TF) coils will consist of seven double pancakes. Each double pancake will have been individually vacuum impregnated and then the seven units assembled together, over-wrapped with glass fabric based insulation and finally vacuum impregnated again to form the TF coil winding pack [1]. The winding pack (WP) will be finally assembled into the coil casing (CC) and to allow for manufacturing tolerances and final geometric definition, a nominal 10 mm gap will exist between the winding pack and the coil case but in practice, this gap may vary between 3 and 15 mm. After assembly, the final step will be to fill the gap with a material that will maintain the final position of the WP and to uniformly transfer load from WP to CC. This paper deals with the selection of materials and techniques to fill the gap and details some of the properties of the chosen material.

Comparative genomic data of the Avian Phylogenomics Project.

PubMed

Zhang, Guojie; Li, Bo; Li, Cai; Gilbert, M Thomas P; Jarvis, Erich D; Wang, Jun

2014-01-01

The evolutionary relationships of modern birds are among the most challenging to understand in systematic biology and have been debated for centuries. To address this challenge, we assembled or collected the genomes of 48 avian species spanning most orders of birds, including all Neognathae and two of the five Palaeognathae orders, and used the genomes to construct a genome-scale avian phylogenetic tree and perform comparative genomics analyses (Jarvis et al. in press; Zhang et al. in press). Here we release assemblies and datasets associated with the comparative genome analyses, which include 38 newly sequenced avian genomes plus previously released or simultaneously released genomes of Chicken, Zebra finch, Turkey, Pigeon, Peregrine falcon, Duck, Budgerigar, Adelie penguin, Emperor penguin and the Medium Ground Finch. We hope that this resource will serve future efforts in phylogenomics and comparative genomics. The 38 bird genomes were sequenced using the Illumina HiSeq 2000 platform and assembled using a whole genome shotgun strategy. The 48 genomes were categorized into two groups according to the N50 scaffold size of the assemblies: a high depth group comprising 23 species sequenced at high coverage (>50X) with multiple insert size libraries resulting in N50 scaffold sizes greater than 1 Mb (except the White-throated Tinamou and Bald Eagle); and a low depth group comprising 25 species sequenced at a low coverage (~30X) with two insert size libraries resulting in an average N50 scaffold size of about 50 kb. Repetitive elements comprised 4%-22% of the bird genomes. The assembled scaffolds allowed the homology-based annotation of 13,000 ~ 17000 protein coding genes in each avian genome relative to chicken, zebra finch and human, as well as comparative and sequence conservation analyses. Here we release full genome assemblies of 38 newly sequenced avian species, link genome assembly downloads for the 7 of the remaining 10 species, and provide a guideline of genomic data that has been generated and used in our Avian Phylogenomics Project. To the best of our knowledge, the Avian Phylogenomics Project is the biggest vertebrate comparative genomics project to date. The genomic data presented here is expected to accelerate further analyses in many fields, including phylogenetics, comparative genomics, evolution, neurobiology, development biology, and other related areas.

A Global Perspective on Human Rights Education. ERIC Digest.

ERIC Educational Resources Information Center

Patrick, John J.

This ERIC Digest outlines what is meant by the phrase human rights and the origin of the concept. It also traces the delineation of the concept of human rights from the 17th century antecedent of "natural rights" to its eventual incarnation as inherent political or personal rights, such as freedom of speech, press, assembly, and…

Freedom of Expression in the American Military: A Communication Modeling Analysis.

ERIC Educational Resources Information Center

Packer, Cathy

A study used a communication perspective to examine the extent to which the civilian/military First Amendment dichotomy is warranted and to add to First Amendment theory the subject of the First Amendment rights of servicemembers. The study was limited to the freedoms of speech, press, assembly, petition, and association. It did not include…

Blueprints for green biotech: development and application of standards for plant synthetic biology.

PubMed

Patron, Nicola J

2016-06-15

Synthetic biology aims to apply engineering principles to the design and modification of biological systems and to the construction of biological parts and devices. The ability to programme cells by providing new instructions written in DNA is a foundational technology of the field. Large-scale de novo DNA synthesis has accelerated synthetic biology by offering custom-made molecules at ever decreasing costs. However, for large fragments and for experiments in which libraries of DNA sequences are assembled in different combinations, assembly in the laboratory is still desirable. Biological assembly standards allow DNA parts, even those from multiple laboratories and experiments, to be assembled together using the same reagents and protocols. The adoption of such standards for plant synthetic biology has been cohesive for the plant science community, facilitating the application of genome editing technologies to plant systems and streamlining progress in large-scale, multi-laboratory bioengineering projects. © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society.

Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture.

PubMed

Eychenne, Thomas; Novikova, Elizaveta; Barrault, Marie-Bénédicte; Alibert, Olivier; Boschiero, Claire; Peixeiro, Nuno; Cornu, David; Redeker, Virginie; Kuras, Laurent; Nicolas, Pierre; Werner, Michel; Soutourina, Julie

2016-09-15

Mediator is a large coregulator complex conserved from yeast to humans and involved in many human diseases, including cancers. Together with general transcription factors, it stimulates preinitiation complex (PIC) formation and activates RNA polymerase II (Pol II) transcription. In this study, we analyzed how Mediator acts in PIC assembly using in vivo, in vitro, and in silico approaches. We revealed an essential function of the Mediator middle module exerted through its Med10 subunit, implicating a key interaction between Mediator and TFIIB. We showed that this Mediator-TFIIB link has a global role on PIC assembly genome-wide. Moreover, the amplitude of Mediator's effect on PIC formation is gene-dependent and is related to the promoter architecture in terms of TATA elements, nucleosome occupancy, and dynamics. This study thus provides mechanistic insights into the coordinated function of Mediator and TFIIB in PIC assembly in different chromatin contexts. © 2016 Eychenne et al.; Published by Cold Spring Harbor Laboratory Press.

Multi-component lightweight gearwheels with deep-drawn wheel body for automotive applications

NASA Astrophysics Data System (ADS)

Benkert, Tim; Hiller, Maria; Volk, Wolfram

2017-09-01

Multi-component gearwheels offer great lightweight opportunities for automotive applications. An assembly of a gear ring and a wheel body joined by press fit replaces the monolithic gearwheel. To save weight, the wheel body uses lightweight design. This lightweight design influences the assembled gearwheel’s mechanical properties like stiffness, weight and torque capacity. Further, the wheel body material influences the mentioned properties as well. In this paper, the effects of the lightweight wheel body manufactured by deep-drawing on the mechanical properties of the assembled gearwheel are investigated. Three different wheel body designs are examined regarding their stiffness and weight compared to a reference gearwheel. Using the best design, the influence of five materials with increasing yield strength on the maximum torque the gearwheel can transmit is studied. All research is done virtually using Abaqus 6.12-3.

Elevated temperature forming method and preheater apparatus

DOEpatents

Krajewski, Paul E; Hammar, Richard Harry; Singh, Jugraj; Cedar, Dennis; Friedman, Peter A; Luo, Yingbing

2013-06-11

An elevated temperature forming system in which a sheet metal workpiece is provided in a first stage position of a multi-stage pre-heater, is heated to a first stage temperature lower than a desired pre-heat temperature, is moved to a final stage position where it is heated to a desired final stage temperature, is transferred to a forming press, and is formed by the forming press. The preheater includes upper and lower platens that transfer heat into workpieces disposed between the platens. A shim spaces the upper platen from the lower platen by a distance greater than a thickness of the workpieces to be heated by the platens and less than a distance at which the upper platen would require an undesirably high input of energy to effectively heat the workpiece without being pressed into contact with the workpiece.

32 CFR 644.104 - Procurement of deed and title assembly.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 4 2013-07-01 2013-07-01 false Procurement of deed and title assembly. 644.104... Procurement of deed and title assembly. In any case in which the Court determines that the United States has... Division, Department of Justice. The title assembly and final title opinion should be forwarded to HQDA...

32 CFR 644.104 - Procurement of deed and title assembly.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 4 2014-07-01 2013-07-01 true Procurement of deed and title assembly. 644.104... Procurement of deed and title assembly. In any case in which the Court determines that the United States has... Division, Department of Justice. The title assembly and final title opinion should be forwarded to HQDA...

32 CFR 644.104 - Procurement of deed and title assembly.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 4 2012-07-01 2011-07-01 true Procurement of deed and title assembly. 644.104... Procurement of deed and title assembly. In any case in which the Court determines that the United States has... Division, Department of Justice. The title assembly and final title opinion should be forwarded to HQDA...

32 CFR 644.104 - Procurement of deed and title assembly.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 4 2011-07-01 2011-07-01 false Procurement of deed and title assembly. 644.104... Procurement of deed and title assembly. In any case in which the Court determines that the United States has... Division, Department of Justice. The title assembly and final title opinion should be forwarded to HQDA...

32 CFR 644.104 - Procurement of deed and title assembly.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 4 2010-07-01 2010-07-01 true Procurement of deed and title assembly. 644.104... Procurement of deed and title assembly. In any case in which the Court determines that the United States has... Division, Department of Justice. The title assembly and final title opinion should be forwarded to HQDA...

Type synthesis for 4-DOF parallel press mechanism using GF set theory

NASA Astrophysics Data System (ADS)

He, Jun; Gao, Feng; Meng, Xiangdun; Guo, Weizhong

2015-07-01

Parallel mechanisms is used in the large capacity servo press to avoid the over-constraint of the traditional redundant actuation. Currently, the researches mainly focus on the performance analysis for some specific parallel press mechanisms. However, the type synthesis and evaluation of parallel press mechanisms is seldom studied, especially for the four degrees of freedom(DOF) press mechanisms. The type synthesis of 4-DOF parallel press mechanisms is carried out based on the generalized function(GF) set theory. Five design criteria of 4-DOF parallel press mechanisms are firstly proposed. The general procedure of type synthesis of parallel press mechanisms is obtained, which includes number synthesis, symmetrical synthesis of constraint GF sets, decomposition of motion GF sets and design of limbs. Nine combinations of constraint GF sets of 4-DOF parallel press mechanisms, ten combinations of GF sets of active limbs, and eleven combinations of GF sets of passive limbs are synthesized. Thirty-eight kinds of press mechanisms are presented and then different structures of kinematic limbs are designed. Finally, the geometrical constraint complexity( GCC), kinematic pair complexity( KPC), and type complexity( TC) are proposed to evaluate the press types and the optimal press type is achieved. The general methodologies of type synthesis and evaluation for parallel press mechanism are suggested.

Steel pin and hanger assembly replacement options : final report.

DOT National Transportation Integrated Search

2017-01-01

A number of steel beam bridges exist in the United States that contain pin and hanger assemblies. Pin and hanger assemblies are fracture critical members whose failure would result in collapse of the bridge or render it unable to perform its expected...

Process development for automated solar cell and module production. Task 4: Automated array assembly

NASA Technical Reports Server (NTRS)

Hagerty, J. J.

1981-01-01

Progress in the development of automated solar cell and module production is reported. The unimate robot is programmed for the final 35 cell pattern to be used in the fabrication of the deliverable modules. The mechanical construction of the automated lamination station and final assembly station phases are completed and the first operational testing is underway. The final controlling program is written and optimized. The glass reinforced concrete (GRC) panels to be used for testing and deliverables are in production. Test routines are grouped together and defined to produce the final control program.

KSC-2011-4047

NASA Image and Video Library

2011-05-25

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, an adult osprey guards its young in a nest built on a platform in the Press Site parking lot. In the background is the 12,300-square-foot NASA logo painted on the side of the Vehicle Assembly Building (VAB). The VAB and Press Site are located at the Turn Basin in Launch Complex 39, making it an ideal osprey nesting place. The Merritt Island National Wildlife Refuge overlaps with Kennedy Space Center property and provides a habitat for many types of wildlife, including the osprey, and 330 species of birds. For information on the refuge, visit http://www.fws.gov/merrittisland/Index.html. Photo credit: NASA/Jack Pfaller

Quality data collection and management technology of aerospace complex product assembly process

NASA Astrophysics Data System (ADS)

Weng, Gang; Liu, Jianhua; He, Yongxi; Zhuang, Cunbo

2017-04-01

Aiming at solving problems of difficult management and poor traceability for discrete assembly process quality data, a data collection and management method is proposed which take the assembly process and BOM as the core. Data collection method base on workflow technology, data model base on BOM and quality traceability of assembly process is included in the method. Finally, assembly process quality data management system is developed and effective control and management of quality information for complex product assembly process is realized.

75 FR 61772 - Certain Coated Paper Suitable for High-Quality Print Graphics Using Sheet-Fed Presses From China...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-06

... INTERNATIONAL TRADE COMMISSION [Investigation Nos. 701-TA-470-471 and 731-TA-1169-1170 (Final)] Certain Coated Paper Suitable for High-Quality Print Graphics Using Sheet-Fed Presses From China and Indonesia AGENCY: United States International Trade Commission. ACTION: Revised schedule for the subject...

75 FR 54650 - Certain Coated Paper Suitable for High-Quality Print Graphics Using Sheet-Fed Presses From China...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-08

... INTERNATIONAL TRADE COMMISSION [Investigation Nos. 701-TA-470-471 and 731-TA-1169-1170 (Final)] Certain Coated Paper Suitable for High-Quality Print Graphics Using Sheet-Fed Presses From China and Indonesia AGENCY: United States International Trade Commission. ACTION: Revised schedule for the subject...

Zirconium Carbide Produced by Spark Plasma Sintering and Hot Pressing: Densification Kinetics, Grain Growth, and Thermal Properties

DOE PAGES

Wei, Xialu; Back, Christina; Izhvanov, Oleg; ...

2016-07-14

Spark plasma sintering (SPS) has been employed to consolidate a micron-sized zirconium carbide (ZrC) powder. ZrC pellets with a variety of relative densities are obtained under different processing parameters. The densification kinetics of ZrC powders subjected to conventional hot pressing and SPS are comparatively studied by applying similar heating and loading profiles. Due to the lack of electric current assistance, the conventional hot pressing appears to impose lower strain rate sensitivity and higher activation energy values than those which correspond to the SPS processing. A finite element simulation is used to analyze the temperature evolution within the volume of ZrCmore » specimens subjected to SPS. The control mechanism for grain growth during the final SPS stage is studied via a recently modified model, in which the grain growth rate dependence on porosity is incorporated. Finally, the constant pressure specific heat and thermal conductivity of the SPS-processed ZrC are determined to be higher than those reported for the hot-pressed ZrC and the benefits of applying SPS are indicated accordingly.« less

Method of preforming and assembling superconducting circuit elements

NASA Astrophysics Data System (ADS)

Haertling, Gene H.; Buckley, John D.

1991-03-01

The invention is a method of preforming and pretesting rigid and discrete superconductor circuit elements to optimize the superconductivity development of the preformed circuit element prior to its assembly, and encapsulation on a substrate and final environmental testing of the assembled ceramic superconductive elements.

Understanding the complex needs of automotive training at final assembly lines.

PubMed

Hermawati, Setia; Lawson, Glyn; D'Cruz, Mirabelle; Arlt, Frank; Apold, Judith; Andersson, Lina; Lövgren, Maria Gink; Malmsköld, Lennart

2015-01-01

Automobile final assembly operators must be highly skilled to succeed in a low automation environment where multiple variants must be assembled in quick succession. This paper presents formal user studies conducted at OPEL and VOLVO Group to identify assembly training needs and a subset of requirements; and to explore potential features of a hypothetical game-based virtual training system. Stakeholder analysis, timeline analysis, link analysis, Hierarchical Task Analysis and thematic content analysis were used to analyse the results of interviews with various stakeholders (17 and 28 participants at OPEL and VOLVO, respectively). The results show that there is a strong case for the implementation of virtual training for assembly tasks. However, it was also revealed that stakeholders would prefer to use a virtual training to complement, rather than replace, training on pre-series vehicles. Copyright © 2014 Elsevier Ltd and The Ergonomics Society. All rights reserved.

Guided and magnetic self-assembly of tunable magnetoceptive gels

NASA Astrophysics Data System (ADS)

Tasoglu, S.; Yu, C. H.; Gungordu, H. I.; Guven, S.; Vural, T.; Demirci, U.

2014-09-01

Self-assembly of components into complex functional patterns at microscale is common in nature, and used increasingly in numerous disciplines such as optoelectronics, microfabrication, sensors, tissue engineering and computation. Here, we describe the use of stable radicals to guide the self-assembly of magnetically tunable gels, which we call ‘magnetoceptive’ materials at the scale of hundreds of microns to a millimeter, each can be programmed by shape and composition, into heterogeneous complex structures. Using paramagnetism of free radicals as a driving mechanism, complex heterogeneous structures are built in the magnetic field generated by permanent magnets. The overall magnetic signature of final structure is erased via an antioxidant vitamin E, subsequent to guided self-assembly. We demonstrate unique capabilities of radicals and antioxidants in fabrication of soft systems with heterogeneity in material properties, such as porosity, elastic modulus and mass density; then in bottom-up tissue engineering and finally, levitational and selective assembly of microcomponents.

Guided and magnetic self-assembly of tunable magnetoceptive gels

PubMed Central

Tasoglu, S.; Yu, C.H.; Gungordu, H.I.; Guven, S.; Vural, T.; Demirci, U.

2014-01-01

Self-assembly of components into complex functional patterns at microscale is common in nature, and used increasingly in numerous disciplines such as optoelectronics, microfabrication, sensors, tissue engineering and computation. Here, we describe the use of stable radicals to guide the self-assembly of magnetically tunable gels, which we call ‘magnetoceptive’ materials at the scale of hundreds of microns to a millimeter, each can be programmed by shape and composition, into heterogeneous complex structures. Using paramagnetism of free radicals as a driving mechanism, complex heterogeneous structures are built in the magnetic field generated by permanent magnets. The overall magnetic signature of final structure is erased via an antioxidant vitamin E, subsequent to guided self-assembly. We demonstrate unique capabilities of radicals and antioxidants in fabrication of soft systems with heterogeneity in material properties, such as porosity, elastic modulus and mass density; then in bottom-up tissue engineering and finally, levitational and selective assembly of microcomponents. PMID:25175148

Pressing movements and perceived force and displacement are influenced by object stiffness.

PubMed

Endo, Hiroshi

2016-09-01

Despite many previous studies on stiffness perception, few have investigated the exploratory procedures involved. This study evaluated whether stiffness range influences pressing movements and perception of force and displacement during stiffness discrimination tasks. Force and displacement data were obtained from 30 participants. Peak values of force and displacement, pressing duration and number of presses were analyzed. Two kinds of subjective evaluations were also recorded: perceived difference in force/displacement used to discriminate between specimens, and perceived effort. Although the number of presses and pressing duration were constant across a wide stiffness range, pressing strength was adjusted for the stiffness of objects, with harder specimens pressed more strongly. Further, even if the stiffnesses of two compared specimens were different, the pressing forces applied to the specimens approached the same magnitude at a higher stiffness range. Differences in force were most easily perceived at lower stiffness ranges, while displacement differences were perceived more readily at higher stiffness ranges. These results were consistent with those of previous studies. Finally, the reasons why stiffness range influenced pressing movements and perceived differences in force/displacement are discussed. Copyright © 2016 Elsevier Inc. All rights reserved.

Understanding Nanoemulsion Formation and Developing a Procedure for Porous Material Growth using Assembled Nanoemulsions

NASA Astrophysics Data System (ADS)

Yeranossian, Vahagn Frounzig

Nanoemulsions as an emerging technology have found many applications in consumer products, drug delivery, and even particle formation. However, knowledge gaps exist in how some of these emulsions are formed, specifically what pathways are traversed to reach the final state. Moreover, how these pathways affect the final properties of the nanoemulsions would affect the applications that these droplets possess. Some nanoemulsions possess unique properties, including the assembly of droplets. While the assembly of droplets is being studied in the Helgeson lab, work must be done to understand how the assembly itself could be used to control the growth of porous materials, such a hydrogels. Thus, this thesis aims to address two factors of nanoemulsions: the formation of water-in-oil nanoemulsions and the use of assemblying droplets in oil-in-water nanoemulsions to form macroporous hydrogels. To elucidate the formation mechanism of water-in-oil nanoemulsions, a combination of dynamic light scattering and small angle neutron scattering were used to study the intermediate and final states of the nanoemulsion during its formation. These nanoemulsions were prepared by slowly adding water to an oil and surfactant mixture and were diluted to effectively measure using scattering techniques without multiple scattering events. To develop a procedure to use assembled nanoemulsions for the growth of porous materials, a combination of optical microscopy and diffusional studies were employed. Optical microscopy images taken at various stages of the procedure help elucidate how the pore sizes of the final porous material is related to the droplet-rich domains of the assembled nanoemulsion. Meanwhile, diffusional measurements help confirm the size and interconnectedness of the macropores. From the work done in the completion of my thesis, the formation mechanism of the water-in-oil nanoemulsion studied has been elucidated. The neutron scattering measurements show that during the formation of the nanoemulsion, a combination of droplets and vesicles form. The presence of vesicles provides insight into how chemical additives in the water would affect the final droplet properties. This insight can be used to design water-in-oil nanoemulsions to be used for the controlled synthesis of solid nanoparticles. Additionally, this work demonstrates a potential procedure for developing macroporous hydrogels using nanoemulsions that are assembled into droplet-rich and droplet-poor domains. Through mild UV cross-linking conditions and mild solvent extraction techniques, the pore sizes could be equivalent to the droplet-rich domain sizes. The final hydrogels can control diffusivity of molecules, giving them potential applications in drug delivery.

KSC-2011-3420

NASA Image and Video Library

2011-05-09

CAPE CANAVERAL, Fla. -- In the Press Site auditorium at NASA's Kennedy Space Center in Florida, NASA managers brief media about the launch status of space shuttle Endeavour's STS-134 mission and announce a new launch date. From left are NASA News Chief Allard Beutel, Space Shuttle Program Launch Integration Manager, Mike Moses and Shuttle Launch Director Mike Leinbach. Technicians replaced and tested the aft load control assembly-2 (ALCA-2) and wiring located in Endeavour's aft avionics bay 5. ALCA-2 distributes power to nine shuttle systems and is believed to have caused fuel line heaters for Endeavour's auxiliary power unit-1 (APU-1) to fail April 29 during the first launch attempt. Launch now is scheduled for May 16 at 8:56 a.m. EDT. Endeavour and its crew will deliver the Express Logistics Carrier-3, Alpha Magnetic Spectrometer-2 (AMS), a high-pressure gas tank and additional spare parts for the Dextre robotic helper to the station. This will be the final spaceflight for Endeavour. For more information visit, www.nasa.gov/mission_pages/shuttle/shuttlemissions/sts134/index.html. Photo credit: NASA/Jack Pfaller

ITER-FEAT vacuum vessel and blanket design features and implications for the R&D programme

NASA Astrophysics Data System (ADS)

Ioki, K.; Dänner, W.; Koizumi, K.; Krylov, V. A.; Cardella, A.; Elio, F.; Onozuka, M.; ITER Joint Central Team; ITER Home Teams

2001-03-01

A configuration in which the vacuum vessel (VV) fits tightly to the plasma aids the passive plasma vertical stability, and ferromagnetic material in the VV reduces the toroidal field ripple. The blanket modules are supported directly by the VV. A full scale VV sector model has provided critical information related to fabrication technology and for testing the magnitude of welding distortions and achievable tolerances. This R&D validated the fundamental feasibility of the double wall VV design. The blanket module configuration consists of a shield body to which a separate first wall is mounted. The separate first wall has a facet geometry consisting of multiple flat panels, where 3-D machining will not be required. A configuration with deep slits minimizes the induced eddy currents and loads. The feasibility and robustness of solid hot isostatic pressing joining were demonstrated in the R&D by manufacturing and testing several small and medium scale mock-ups and finally two prototypes. Remote handling tests and assembly tests of a blanket module have demonstrated the basic feasibility of its installation and removal.

High-Pressure Turbulent Flame Speeds and Chemical Kinetics of Syngas Blends with and without Impurities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peterson, Eric; Mathieu, Olivier; Morones, Anibal

2014-12-01

This Topical Report documents the first year of the project, from October 1, 2013 through September 30, 2014. Efforts for this project included experiments to characterize the atmospheric-pressure turbulent flame speed vessel over a range of operating conditions (fan speeds and turbulent length scales). To this end, a new LDV system was acquired and set up for the detailed characterization of the turbulence field. Much progress was made in the area of impurity kinetics, which included a numerical study of the effect of impurities such as NO2, NO, H2S, and NH3 on ignition delay times and laminar flame speeds ofmore » syngas blends at engine conditions. Experiments included a series of laminar flame speed measurements for syngas (CO/H2) blends with various levels of CH4 and C2H6 addition, and the results were compared to the chemical kinetics model of NUI Galway. Also, a final NOx kinetics mechanism including ammonia was assembled, and a journal paper was written and is now in press. Overall, three journal papers and six conference papers related to this project were published this year. Finally, much progress was made on the design of the new high-pressure turbulent flame speed facility. An overall design that includes a venting system was decided upon, and the detailed design is in progress.« less

TOPICAL REVIEW: Self-assembly from milli- to nanoscales: methods and applications

NASA Astrophysics Data System (ADS)

Mastrangeli, M.; Abbasi, S.; Varel, C.; Van Hoof, C.; Celis, J.-P.; Böhringer, K. F.

2009-08-01

The design and fabrication techniques for microelectromechanical systems (MEMS) and nanodevices are progressing rapidly. However, due to material and process flow incompatibilities in the fabrication of sensors, actuators and electronic circuitry, a final packaging step is often necessary to integrate all components of a heterogeneous microsystem on a common substrate. Robotic pick-and-place, although accurate and reliable at larger scales, is a serial process that downscales unfavorably due to stiction problems, fragility and sheer number of components. Self-assembly, on the other hand, is parallel and can be used for device sizes ranging from millimeters to nanometers. In this review, the state-of-the-art in methods and applications for self-assembly is reviewed. Methods for assembling three-dimensional (3D) MEMS structures out of two-dimensional (2D) ones are described. The use of capillary forces for folding 2D plates into 3D structures, as well as assembling parts onto a common substrate or aggregating parts to each other into 2D or 3D structures, is discussed. Shape matching and guided assembly by magnetic forces and electric fields are also reviewed. Finally, colloidal self-assembly and DNA-based self-assembly, mainly used at the nanoscale, are surveyed, and aspects of theoretical modeling of stochastic assembly processes are discussed.

Self-assembly from milli- to nanoscales: methods and applications

PubMed Central

Mastrangeli, M; Abbasi, S; Varel, C; Van Hoof, C; Celis, J-P; Böhringer, K F

2009-01-01

The design and fabrication techniques for microelectromechanical systems (MEMS) and nanodevices are progressing rapidly. However, due to material and process flow incompatibilities in the fabrication of sensors, actuators and electronic circuitry, a final packaging step is often necessary to integrate all components of a heterogeneous microsystem on a common substrate. Robotic pick-and-place, although accurate and reliable at larger scales, is a serial process that downscales unfavorably due to stiction problems, fragility and sheer number of components. Self-assembly, on the other hand, is parallel and can be used for device sizes ranging from millimeters to nanometers. In this review, the state-of-the-art in methods and applications for self-assembly is reviewed. Methods for assembling three-dimensional (3D) MEMS structures out of two-dimensional (2D) ones are described. The use of capillary forces for folding 2D plates into 3D structures, as well as assembling parts onto a common substrate or aggregating parts to each other into 2D or 3D structures, is discussed. Shape matching and guided assembly by magnetic forces and electric fields are also reviewed. Finally, colloidal self-assembly and DNA-based self-assembly, mainly used at the nanoscale, are surveyed, and aspects of theoretical modeling of stochastic assembly processes are discussed. PMID:20209016

Certain Unalienable Rights. Materials for Using American Issues Forum in the American History Classroom, Topic III.

ERIC Educational Resources Information Center

New York State Education Dept., Albany. Div. of General Education.

This booklet presents a set of secondary level classroom strategies for examining American history in light of issues identified by the American Issues Forum. Emphasis is on "certain inalienable rights" of citizens. This topic is covered in four sections: freedom of speech, assembly, and religion; freedom of the press; freedom of search…

Multiciliated Cells in Animals.

PubMed

Meunier, Alice; Azimzadeh, Juliette

2016-12-01

Many animal cells assemble single cilia involved in motile and/or sensory functions. In contrast, multiciliated cells (MCCs) assemble up to 300 motile cilia that beat in a coordinate fashion to generate a directional fluid flow. In the human airways, the brain, and the oviduct, MCCs allow mucus clearance, cerebrospinal fluid circulation, and egg transportation, respectively. Impairment of MCC function leads to chronic respiratory infections and increased risks of hydrocephalus and female infertility. MCC differentiation during development or repair involves the activation of a regulatory cascade triggered by the inhibition of Notch activity in MCC progenitors. The downstream events include the simultaneous assembly of a large number of basal bodies (BBs)-from which cilia are nucleated-in the cytoplasm of the differentiating MCCs, their migration and docking at the plasma membrane associated to an important remodeling of the actin cytoskeleton, and the assembly and polarization of motile cilia. The direction of ciliary beating is coordinated both within cells and at the tissue level by a combination of planar polarity cues affecting BB position and hydrodynamic forces that are both generated and sensed by the cilia. Herein, we review the mechanisms controlling the specification and differentiation of MCCs and BB assembly and organization at the apical surface, as well as ciliary assembly and coordination in MCCs. Copyright © 2016 Cold Spring Harbor Laboratory Press; all rights reserved.

Assembly constraints drive co-evolution among ribosomal constituents.

PubMed

Mallik, Saurav; Akashi, Hiroshi; Kundu, Sudip

2015-06-23

Ribosome biogenesis, a central and essential cellular process, occurs through sequential association and mutual co-folding of protein-RNA constituents in a well-defined assembly pathway. Here, we construct a network of co-evolving nucleotide/amino acid residues within the ribosome and demonstrate that assembly constraints are strong predictors of co-evolutionary patterns. Predictors of co-evolution include a wide spectrum of structural reconstitution events, such as cooperativity phenomenon, protein-induced rRNA reconstitutions, molecular packing of different rRNA domains, protein-rRNA recognition, etc. A correlation between folding rate of small globular proteins and their topological features is known. We have introduced an analogous topological characteristic for co-evolutionary network of ribosome, which allows us to differentiate between rRNA regions subjected to rapid reconstitutions from those hindered by kinetic traps. Furthermore, co-evolutionary patterns provide a biological basis for deleterious mutation sites and further allow prediction of potential antibiotic targeting sites. Understanding assembly pathways of multicomponent macromolecules remains a key challenge in biophysics. Our study provides a 'proof of concept' that directly relates co-evolution to biophysical interactions during multicomponent assembly and suggests predictive power to identify candidates for critical functional interactions as well as for assembly-blocking antibiotic target sites. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

Multiple hybrid de novo genome assembly of finger millet, an orphan allotetraploid crop.

PubMed

Hatakeyama, Masaomi; Aluri, Sirisha; Balachadran, Mathi Thumilan; Sivarajan, Sajeevan Radha; Patrignani, Andrea; Grüter, Simon; Poveda, Lucy; Shimizu-Inatsugi, Rie; Baeten, John; Francoijs, Kees-Jan; Nataraja, Karaba N; Reddy, Yellodu A Nanja; Phadnis, Shamprasad; Ravikumar, Ramapura L; Schlapbach, Ralph; Sreeman, Sheshshayee M; Shimizu, Kentaro K

2017-09-05

Finger millet (Eleusine coracana (L.) Gaertn) is an important crop for food security because of its tolerance to drought, which is expected to be exacerbated by global climate changes. Nevertheless, it is often classified as an orphan/underutilized crop because of the paucity of scientific attention. Among several small millets, finger millet is considered as an excellent source of essential nutrient elements, such as iron and zinc; hence, it has potential as an alternate coarse cereal. However, high-quality genome sequence data of finger millet are currently not available. One of the major problems encountered in the genome assembly of this species was its polyploidy, which hampers genome assembly compared with a diploid genome. To overcome this problem, we sequenced its genome using diverse technologies with sufficient coverage and assembled it via a novel multiple hybrid assembly workflow that combines next-generation with single-molecule sequencing, followed by whole-genome optical mapping using the Bionano Irys® system. The total number of scaffolds was 1,897 with an N50 length >2.6 Mb and detection of 96% of the universal single-copy orthologs. The majority of the homeologs were assembled separately. This indicates that the proposed workflow is applicable to the assembly of other allotetraploid genomes. © The Author 2017. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

Evaluation of the performance of a press-lam timber bridge : bridge performance and load test after five years, final report.

DOT National Transportation Integrated Search

1982-01-01

The report describes the results of the load tests and summarizes the collection of data on the dimensional changes and moisture content of the press-lain members and the number and types of vehicles using the bridge. The results of the load tests su...

Icarus: visualizer for de novo assembly evaluation.

PubMed

Mikheenko, Alla; Valin, Gleb; Prjibelski, Andrey; Saveliev, Vladislav; Gurevich, Alexey

2016-11-01

: Data visualization plays an increasingly important role in NGS data analysis. With advances in both sequencing and computational technologies, it has become a new bottleneck in genomics studies. Indeed, evaluation of de novo genome assemblies is one of the areas that can benefit from the visualization. However, even though multiple quality assessment methods are now available, existing visualization tools are hardly suitable for this purpose. Here, we present Icarus-a novel genome visualizer for accurate assessment and analysis of genomic draft assemblies, which is based on the tool QUAST. Icarus can be used in studies where a related reference genome is available, as well as for non-model organisms. The tool is available online and as a standalone application. http://cab.spbu.ru/software/icarus CONTACT: aleksey.gurevich@spbu.ruSupplementary information: Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

SeqLib: a C ++ API for rapid BAM manipulation, sequence alignment and sequence assembly.

PubMed

Wala, Jeremiah; Beroukhim, Rameen

2017-03-01

We present SeqLib, a C ++ API and command line tool that provides a rapid and user-friendly interface to BAM/SAM/CRAM files, global sequence alignment operations and sequence assembly. Four C libraries perform core operations in SeqLib: HTSlib for BAM access, BWA-MEM and BLAT for sequence alignment and Fermi for error correction and sequence assembly. Benchmarking indicates that SeqLib has lower CPU and memory requirements than leading C ++ sequence analysis APIs. We demonstrate an example of how minimal SeqLib code can extract, error-correct and assemble reads from a CRAM file and then align with BWA-MEM. SeqLib also provides additional capabilities, including chromosome-aware interval queries and read plotting. Command line tools are available for performing integrated error correction, micro-assemblies and alignment. SeqLib is available on Linux and OSX for the C ++98 standard and later at github.com/walaj/SeqLib. SeqLib is released under the Apache2 license. Additional capabilities for BLAT alignment are available under the BLAT license. jwala@broadinstitue.org ; rameen@broadinstitute.org. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com

High power s-band vacuum load

DOE Office of Scientific and Technical Information (OSTI.GOV)

Neubauer, Michael; Dudas, Alan; Krasnykh, Anatoly

Through a combination of experimentation and calculation the components of a novel room temperature dry load were successfully fabricated. These components included lossy ceramic cylinders of various lengths, thicknesses, and percent of silicon carbide (SiC). The cylinders were then assembled into stainless steel compression rings by differential heating of the parts and a special fixture. Post machining of this assembly provided a means for a final weld. The ring assemblies were then measured for S-parameters, individually and in pairs using a low-cost TE10 rectangular to TE01 circular waveguide adapter specially designed to be part of the final load assembly. Matchedmore » pairs of rings were measured for assembly into the final load, and a sliding short designed and fabricated to assist in determining the desired short location in the final assembly. The plan for the project was for Muons, Inc. to produce prototype loads for long-term testing at SLAC. The STTR funds for SLAC were to upgrade and operate their test station to ensure that the loads would satisfy their requirements. Phase III was to be the sale to SLAC of loads that Muons, Inc. would manufacture. However, an alternate solution that involved a rebuild of the old loads, reduced SLAC budget projections, and a relaxed time for the replacement of all loads meant that in-house labor will be used to do the upgrade without the need for the loads developed in this project. Consequently, the project was terminated before the long term testing was initiated. However, SLAC can use the upgraded test stand to compare the long-term performance of the ones produced in this project with their rebuilt loads when they are available.« less

Cell-assembly coding in several memory processes.

PubMed

Sakurai, Y

1998-01-01

The present paper discusses why the cell assembly, i.e., an ensemble population of neurons with flexible functional connections, is a tenable view of the basic code for information processes in the brain. The main properties indicating the reality of cell-assembly coding are neurons overlaps among different assemblies and connection dynamics within and among the assemblies. The former can be detected as multiple functions of individual neurons in processing different kinds of information. Individual neurons appear to be involved in multiple information processes. The latter can be detected as changes of functional synaptic connections in processing different kinds of information. Correlations of activity among some of the recorded neurons appear to change in multiple information processes. Recent experiments have compared several different memory processes (tasks) and detected these two main properties, indicating cell-assembly coding of memory in the working brain. The first experiment compared different types of processing of identical stimuli, i.e., working memory and reference memory of auditory stimuli. The second experiment compared identical processes of different types of stimuli, i.e., discriminations of simple auditory, simple visual, and configural auditory-visual stimuli. The third experiment compared identical processes of different types of stimuli with or without temporal processing of stimuli, i.e., discriminations of elemental auditory, configural auditory-visual, and sequential auditory-visual stimuli. Some possible features of the cell-assembly coding, especially "dual coding" by individual neurons and cell assemblies, are discussed for future experimental approaches. Copyright 1998 Academic Press.

Fracture toughness of CIP-HIP (cold isostatic pressed - hot isostatic pressed) beryllium at elevated temperatures. Final report, 13 May 1980-13 February 1981

DOE Office of Scientific and Technical Information (OSTI.GOV)

Barker, L.M.; Jones, A.H.

1986-04-01

The fracture toughness of CIP-HIP (cold isostatic pressed-hot isostatic pressed) beryllium was determined using the short-bar fracture-toughness (K/sub IcSB/) method. The K/sub IcSB/ value measured was 10.96 MPa x the square root of m at room temperature. This falls well within the expected range of 9 to 12 MPa x the square root of m as observed from previous fracture toughness measurements of beryllium. Toughness increased rapidly between 400 F and 500 F reaching a value of 16.7 MPa x the square root of m at 500 F.

A Synthetic Circuit for Mercury Bioremediation Using Self-Assembling Functional Amyloids.

PubMed

Tay, Pei Kun R; Nguyen, Peter Q; Joshi, Neel S

2017-10-20

Synthetic biology approaches to bioremediation are a key sustainable strategy to leverage the self-replicating and programmable aspects of biology for environmental stewardship. The increasing spread of anthropogenic mercury pollution into our habitats and food chains is a pressing concern. Here, we explore the use of programmed bacterial biofilms to aid in the sequestration of mercury. We demonstrate that by integrating a mercury-responsive promoter and an operon encoding a mercury-absorbing self-assembling extracellular protein nanofiber, we can engineer bacteria that can detect and sequester toxic Hg 2+ ions from the environment. This work paves the way for the development of on-demand biofilm living materials that can operate autonomously as heavy-metal absorbents.

Transition joints between Zircaloy-2 and stainless steel by diffusion bonding

NASA Astrophysics Data System (ADS)

Bhanumurthy, K.; Krishnan, J.; Kale, G. B.; Banerjee, S.

1994-11-01

The diffusion bonding between Zircaloy-2 and stainless steel (AISI 304L) using niobium, nickel and copper as intermediate layers has been investigated in the temperature range of 750 to 900°C. Bonding was carried out in a vacuum hot press, under compressive loading. Electron probe microanalysis and metallographic analysis showed a good metallurgical compatibility and also indicated the absence of discontunities, micropores and intermetallic compounds at various interfaces. The bond strength of the diffusion bonded assembly was found to be about 400 MPa for the couples bonded at 870°C for 2 h. The dimple structure on the fractured surface is indicative of the ductile mode of failure of the bonded assembly.

Hot embossing and thermal bonding of poly(methyl methacrylate) microfluidic chips using positive temperature coefficient ceramic heater.

PubMed

Wang, Xia; Zhang, Luyan; Chen, Gang

2011-11-01

As a self-regulating heating device, positive temperature coefficient ceramic heater was employed for hot embossing and thermal bonding of poly(methyl methacrylate) microfluidic chip because it supplied constant-temperature heating without electrical control circuits. To emboss a channel plate, a piece of poly(methyl methacrylate) plate was sandwiched between a template and a microscopic glass slide on a positive temperature coefficient ceramic heater. All the assembled components were pressed between two elastic press heads of a spring-driven press while a voltage was applied to the heater for 10 min. Subsequently, the embossed poly(methyl methacrylate) plate bearing negative relief of channel networks was bonded with a piece of poly(methyl methacrylate) cover sheet to obtain a complete microchip using a positive temperature coefficient ceramic heater and a spring-driven press. High quality microfluidic chips fabricated by using the novel embossing/bonding device were successfully applied in the electrophoretic separation of three cations. Positive temperature coefficient ceramic heater indicates great promise for the low-cost production of poly(methyl methacrylate) microchips and should find wide applications in the fabrication of other thermoplastic polymer microfluidic devices.

Evaluation of GRCh38 and de novo haploid genome assemblies demonstrates the enduring quality of the reference assembly.

PubMed

Schneider, Valerie A; Graves-Lindsay, Tina; Howe, Kerstin; Bouk, Nathan; Chen, Hsiu-Chuan; Kitts, Paul A; Murphy, Terence D; Pruitt, Kim D; Thibaud-Nissen, Françoise; Albracht, Derek; Fulton, Robert S; Kremitzki, Milinn; Magrini, Vincent; Markovic, Chris; McGrath, Sean; Steinberg, Karyn Meltz; Auger, Kate; Chow, William; Collins, Joanna; Harden, Glenn; Hubbard, Timothy; Pelan, Sarah; Simpson, Jared T; Threadgold, Glen; Torrance, James; Wood, Jonathan M; Clarke, Laura; Koren, Sergey; Boitano, Matthew; Peluso, Paul; Li, Heng; Chin, Chen-Shan; Phillippy, Adam M; Durbin, Richard; Wilson, Richard K; Flicek, Paul; Eichler, Evan E; Church, Deanna M

2017-05-01

The human reference genome assembly plays a central role in nearly all aspects of today's basic and clinical research. GRCh38 is the first coordinate-changing assembly update since 2009; it reflects the resolution of roughly 1000 issues and encompasses modifications ranging from thousands of single base changes to megabase-scale path reorganizations, gap closures, and localization of previously orphaned sequences. We developed a new approach to sequence generation for targeted base updates and used data from new genome mapping technologies and single haplotype resources to identify and resolve larger assembly issues. For the first time, the reference assembly contains sequence-based representations for the centromeres. We also expanded the number of alternate loci to create a reference that provides a more robust representation of human population variation. We demonstrate that the updates render the reference an improved annotation substrate, alter read alignments in unchanged regions, and impact variant interpretation at clinically relevant loci. We additionally evaluated a collection of new de novo long-read haploid assemblies and conclude that although the new assemblies compare favorably to the reference with respect to continuity, error rate, and gene completeness, the reference still provides the best representation for complex genomic regions and coding sequences. We assert that the collected updates in GRCh38 make the newer assembly a more robust substrate for comprehensive analyses that will promote our understanding of human biology and advance our efforts to improve health. © 2017 Schneider et al.; Published by Cold Spring Harbor Laboratory Press.

Pleated metal bipolar assembly

DOEpatents

Wilson, Mahlon S.; Zawodzinski, Christine

2001-01-01

A thin low-cost bipolar plate for an electrochemical cell is formed from a polymer support plate with first flow channels on a first side of the support plate and second flow channels on a second side of the support plate, where the first flow channels and second flow channels have intersecting locations and have a depth effective to form openings through the support plate at the intersecting locations. A first foil of electrically conductive material is pressed into the first flow channels. A second foil of electrically conductive material pressed into the second flow channels so that electrical contact is made between the first and second foils at the openings through the support plate. A particular application of the bipolar plate is in polymer electrolyte fuel cells.

Process Development for Automated Solar Cell and Module Production. Task 4: Automated Array Assembly

NASA Technical Reports Server (NTRS)

Hagerty, J. J.

1981-01-01

The Automated Lamination Station is mechanically complete and is currently undergoing final wiring. The high current driver and isolator boards have been completed and installed, and the main interface board is under construction. The automated vacuum chamber has had a minor redesign to increase stiffness and improve the cover open/close mechanism. Design of the Final Assembly Station has been completed and construction is underway.

Microfluidic device for the assembly and transport of microparticles

DOEpatents

James, Conrad D [Albuquerque, NM; Kumar, Anil [Framingham, MA; Khusid, Boris [New Providence, NJ; Acrivos, Andreas [Stanford, CA

2010-06-29

A microfluidic device comprising independently addressable arrays of interdigitated electrodes can be used to assembly and transport large-scale microparticle structures. The device and method uses collective phenomena in a negatively polarized suspension exposed to a high-gradient strong ac electric field to assemble the particles into predetermined locations and then transport them collectively to a work area for final assembly by sequentially energizing the electrode arrays.

Mechanical Properties of Copper Processed by Equal Channel Angular Pressing

NASA Astrophysics Data System (ADS)

Sülleiová, K.; Ballóková, B.; Besterci, M.; Kvačkaj, T.

2017-12-01

The development of the nanostructure in commercial pure copper and the strength and ductility after severe plastic deformation (SPD) with the technology of equal channel angular pressing (ECAP) are analysed. Experimental results and analyses showed that both strength and ductility can be increased simultaneously by SPD. The final grain size decreased from the initial 50μm by SPD to 100-300 nm after 10 passes. An increase of the ductility together with an increase of strength caused by SPD are explained by a strong grain refinement and by a dynamic equilibrium of weakening and strengthening, and it is visible on the final static tensile test stress-strain charts.

75 FR 65023 - Notice of Issuance of Final Determination Concerning Certain Heating Boilers

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-21

... of the heat exchanger, the gas train, electronics and controls, and the combustion fan. Assembly of... the heat exchanger are of U.S. origin. The gas train assembly requires fitting the components together... stage. The sub-assembly stage has three processes: the gas train, electronics and controls, and the...

77 FR 63788 - Crystalline Silicon Photovoltaic Cells, Whether or Not Assembled Into Modules, From the People's...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-17

... Photovoltaic Cells, Whether or Not Assembled Into Modules, From the People's Republic of China: Final... (solar cells) from the People's Republic of China (PRC). The Department further determines that there... Cells, Whether or Not Assembled Into Modules, from the People's Republic of China (Decision Memorandum...

Genome sequence of pacific abalone (Haliotis discus hannai): the first draft genome in family Haliotidae.

PubMed

Nam, Bo-Hye; Kwak, Woori; Kim, Young-Ok; Kim, Dong-Gyun; Kong, Hee Jeong; Kim, Woo-Jin; Kang, Jeong-Ha; Park, Jung Youn; An, Cheul Min; Moon, Ji-Young; Park, Choul Ji; Yu, Jae Woong; Yoon, Joon; Seo, Minseok; Kim, Kwondo; Kim, Duk Kyung; Lee, SaetByeol; Sung, Samsun; Lee, Chul; Shin, Younhee; Jung, Myunghee; Kang, Byeong-Chul; Shin, Ga-Hee; Ka, Sojeong; Caetano-Anolles, Kelsey; Cho, Seoae; Kim, Heebal

2017-05-01

Abalones are large marine snails in the family Haliotidae and the genus Haliotis belonging to the class Gastropoda of the phylum Mollusca. The family Haliotidae contains only one genus, Haliotis, and this single genus is known to contain several species of abalone. With 18 additional subspecies, the most comprehensive treatment of Haliotidae considers 56 species valid [ 1 ]. Abalone is an economically important fishery and aquaculture animal that is considered a highly prized seafood delicacy. The total global supply of abalone has increased 5-fold since the 1970s and farm production increased explosively from 50 mt to 103 464 mt in the past 40 years. Additionally, researchers have recently focused on abalone given their reported tumor suppression effect. However, despite the valuable features of this marine animal, no genomic information is available for the Haliotidae family and related research is still limited. To construct the H . discus hannai genome, a total of 580-G base pairs using Illumina and Pacbio platforms were generated with 322-fold coverage based on the 1.8-Gb estimated genome size of H . discus hannai using flow cytometry. The final genome assembly consisted of 1.86 Gb with 35 450 scaffolds (>2 kb). GC content level was 40.51%, and the N50 length of assembled scaffolds was 211 kb. We identified 29 449 genes using Evidence Modeler based on the gene information from ab initio prediction, protein homology with known genes, and transcriptome evidence of RNA-seq. Here we present the first Haliotidae genome, H . discus hannai , with sequencing data, assembly, and gene annotation information. This will be helpful for resolving the lack of genomic information in the Haliotidae family as well as providing more opportunities for understanding gastropod evolution. © The Authors 2017. Published by Oxford University Press.

Automation or De-automation

NASA Astrophysics Data System (ADS)

Gorlach, Igor; Wessel, Oliver

2008-09-01

In the global automotive industry, for decades, vehicle manufacturers have continually increased the level of automation of production systems in order to be competitive. However, there is a new trend to decrease the level of automation, especially in final car assembly, for reasons of economy and flexibility. In this research, the final car assembly lines at three production sites of Volkswagen are analysed in order to determine the best level of automation for each, in terms of manufacturing costs, productivity, quality and flexibility. The case study is based on the methodology proposed by the Fraunhofer Institute. The results of the analysis indicate that fully automated assembly systems are not necessarily the best option in terms of cost, productivity and quality combined, which is attributed to high complexity of final car assembly systems; some de-automation is therefore recommended. On the other hand, the analysis shows that low automation can result in poor product quality due to reasons related to plant location, such as inadequate workers' skills, motivation, etc. Hence, the automation strategy should be formulated on the basis of analysis of all relevant aspects of the manufacturing process, such as costs, quality, productivity and flexibility in relation to the local context. A more balanced combination of automated and manual assembly operations provides better utilisation of equipment, reduces production costs and improves throughput.

Democratic Reforms in Taiwan: Issues for Congress

DTIC Science & Technology

2010-05-26

stationed in Taiwan were happy under martial law. For local citizens, the Taiwan Garrison Command tried defendants for alleged civilian crimes ...of dissidents; sentencing of an average of 10 years for several hundred political prisoners; use of martial law to try a variety of crimes in...military courts; limited freedom of speech , political assembly, freedom of the press, and labor strikes; lengthy detentions incommunicado and without

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pintauro, Peter N.; Ballengee, Jason; Brodt, Matthew

In one aspect of the present invention, a method of fabricating a fuel cell membrane-electrode-assembly (MEA) having an anode electrode, a cathode electrode, and a membrane disposed between the anode electrode and the cathode electrode, includes fabricating each of the anode electrode, the cathode electrode, and the membrane separately by electrospinning; and placing the membrane between the anode electrode and the cathode electrode, and pressing then together to form the fuel cell MEA.

Reversible Self-Assembly of 3D Architectures Actuated by Responsive Polymers.

PubMed

Zhang, Cheng; Su, Jheng-Wun; Deng, Heng; Xie, Yunchao; Yan, Zheng; Lin, Jian

2017-11-29

An assembly of three-dimensional (3D) architectures with defined configurations has important applications in broad areas. Among various approaches of constructing 3D structures, a stress-driven assembly provides the capabilities of creating 3D architectures in a broad range of functional materials with unique merits. However, 3D architectures built via previous methods are simple, irreversible, or not free-standing. Furthermore, the substrates employed for the assembly remain flat, thus not involved as parts of the final 3D architectures. Herein, we report a reversible self-assembly of various free-standing 3D architectures actuated by the self-folding of smart polymer substrates with programmed geometries. The strategically designed polymer substrates can respond to external stimuli, such as organic solvents, to initiate the 3D assembly process and subsequently become the parts of the final 3D architectures. The self-assembly process is highly controllable via origami and kirigami designs patterned by direct laser writing. Self-assembled geometries include 3D architectures such as "flower", "rainbow", "sunglasses", "box", "pyramid", "grating", and "armchair". The reported self-assembly also shows wide applicability to various materials including epoxy, polyimide, laser-induced graphene, and metal films. The device examples include 3D architectures integrated with a micro light-emitting diode and a flex sensor, indicting the potential applications in soft robotics, bioelectronics, microelectromechanical systems, and others.

Method of making membrane-electrode assemblies for electrochemical cells and assemblies made thereby

DOEpatents

Swathirajan, Sundararajan; Mikhail, Youssef M.

1994-01-01

A method of making a combination, unitary, membrane and electrode assembly having a solid polymer electrolyte membrane, and first and second electrodes at least partially embedded in opposed surfaces of the membrane. The electrodes each comprise a respective group of finely divided carbon particles, very finely divided catalytic particles supported on internal and external surfaces of the carbon particles and a proton conductive material intermingled with the catalytic and carbon particles. A first group of finely divided carbon particles forming the first electrode has greater water attraction and retention properties, and is more hydrophilic than a second group of carbon particles forming the second electrode. In a preferred method, the membrane electrode assembly of the invention is prepared by forming a slurry of proton conductive material and at least one group of the carbon and catalyst particles. The slurry is applied to the opposed surfaces of the membrane and heated while being pressed to the membrane for a time and at a temperature and compressive load sufficient to embed at least a portion of the particles into the membrane.

Programmable formation of catalytic RNA triangles and squares by assembling modular RNA enzymes.

PubMed

Oi, Hiroki; Fujita, Daisuke; Suzuki, Yuki; Sugiyama, Hiroshi; Endo, Masayuki; Matsumura, Shigeyoshi; Ikawa, Yoshiya

2017-05-01

RNA is a biopolymer that is attractive for constructing nano-scale objects with complex structures. Three-dimensional (3D) structures of naturally occurring RNAs often have modular architectures. The 3D structure of a group I (GI) ribozyme from Tetrahymena has a typical modular architecture, which can be separated into two structural modules (ΔP5 and P5abc). The fully active ribozyme can be reconstructed by assembling the two separately prepared modules through highly specific and strong assembly between ΔP5 ribozyme and P5abc RNA. Such non-covalent assembly of the two modules allows the design of polygonal RNA nano-structures. Through rational redesign of the parent GI ribozyme, we constructed variant GI ribozymes as unit RNAs for polygonal-shaped (closed) oligomers with catalytic activity. Programmed trimerization and tetramerization of the unit RNAs afforded catalytically active nano-sized RNA triangles and squares, the structures of which were directly observed by atomic force microscopy (AFM). © The Authors 2017. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Optimizing DNA assembly based on statistical language modelling.

PubMed

Fang, Gang; Zhang, Shemin; Dong, Yafei

2017-12-15

By successively assembling genetic parts such as BioBrick according to grammatical models, complex genetic constructs composed of dozens of functional blocks can be built. However, usually every category of genetic parts includes a few or many parts. With increasing quantity of genetic parts, the process of assembling more than a few sets of these parts can be expensive, time consuming and error prone. At the last step of assembling it is somewhat difficult to decide which part should be selected. Based on statistical language model, which is a probability distribution P(s) over strings S that attempts to reflect how frequently a string S occurs as a sentence, the most commonly used parts will be selected. Then, a dynamic programming algorithm was designed to figure out the solution of maximum probability. The algorithm optimizes the results of a genetic design based on a grammatical model and finds an optimal solution. In this way, redundant operations can be reduced and the time and cost required for conducting biological experiments can be minimized. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Method of making membrane-electrode assemblies for electrochemical cells and assemblies made thereby

DOEpatents

Swathirajan, S.; Mikhail, Y.M.

1994-05-31

A method is described for making a combination, unitary, membrane and electrode assembly having a solid polymer electrolyte membrane, and first and second electrodes at least partially embedded in opposed surfaces of the membrane. The electrodes each comprise a respective group of finely divided carbon particles, very finely divided catalytic particles supported on internal and external surfaces of the carbon particles and a proton conductive material intermingled with the catalytic and carbon particles. A first group of finely divided carbon particles forming the first electrode has greater water attraction and retention properties, and is more hydrophilic than a second group of carbon particles forming the second electrode. In a preferred method, the membrane electrode assembly of the invention is prepared by forming a slurry of proton conductive material and at least one group of the carbon and catalyst particles. The slurry is applied to the opposed surfaces of the membrane and heated while being pressed to the membrane for a time and at a temperature and compressive load sufficient to embed at least a portion of the particles into the membrane. 10 figs.

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 flight engineer Steve Swanson of NASA and Soyuz commander Aleksander Skvortsov of the Russian Federal Space Agency, Roscosmos are seen in quarantine, behind glass, during the final press conference be Monday, March 24, 2014, at the Cosmonaut Hotel in Baikonur, Kazakhstan. The mission to the International Space Station is set to launch March 26 from the Baikonur Cosmodrome. Photo Credit: (NASA/Joel Kowsky)

Expedition 38 Press Conference

NASA Image and Video Library

2013-11-06

Expedition 38 Soyuz Commander Mikhail Tyurin of Roscosmos, center, talks as Flight Engineer Koichi Wakata of the Japan Aerospace Exploration Agency, left, and, Flight Engineer Rick Mastracchio of NASA listen, while quarantine behind glass, during the final press conference held a day ahead of their launch to the International Space Station, Wednesday, Nov. 6, 2013 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Bill Ingalls)

STS_135_ MEDIA

NASA Image and Video Library

2011-06-30

JSC2011-E-060796 (30 June 2011) --- Reporter Mark Carreau of Aviation Week takes notes during the STS-135 crew media briefing at NASA?s Johnson Space Center on June 30, 2011. The press conference provided the last scheduled opportunity for a large group of press to speak with the crew before the final launch on July 8. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

Using Maximal Isometric Force to Determine the Optimal Load for Measuring Dynamic Muscle Power

NASA Technical Reports Server (NTRS)

Spiering, Barry A.; Lee, Stuart M. C.; Mulavara, Ajitkumar P.; Bentley, Jason R.; Nash, Roxanne E.; Sinka, Joseph; Bloomberg, Jacob J.

2009-01-01

Maximal power output occurs when subjects perform ballistic exercises using loads of 30-50% of one-repetition maximum (1-RM). However, performing 1-RM testing prior to power measurement requires considerable time, especially when testing involves multiple exercises. Maximal isometric force (MIF), which requires substantially less time to measure than 1-RM, might be an acceptable alternative for determining the optimal load for power testing. PURPOSE: To determine the optimal load based on MIF for maximizing dynamic power output during leg press and bench press exercises. METHODS: Twenty healthy volunteers (12 men and 8 women; mean +/- SD age: 31+/-6 y; body mass: 72 +/- 15 kg) performed isometric leg press and bench press movements, during which MIF was measured using force plates. Subsequently, subjects performed ballistic leg press and bench press exercises using loads corresponding to 20%, 30%, 40%, 50%, and 60% of MIF presented in randomized order. Maximal instantaneous power was calculated during the ballistic exercise tests using force plates and position transducers. Repeated-measures ANOVA and Fisher LSD post hoc tests were used to determine the load(s) that elicited maximal power output. RESULTS: For the leg press power test, six subjects were unable to be tested at 20% and 30% MIF because these loads were less than the lightest possible load (i.e., the weight of the unloaded leg press sled assembly [31.4 kg]). For the bench press power test, five subjects were unable to be tested at 20% MIF because these loads were less than the weight of the unloaded aluminum bar (i.e., 11.4 kg). Therefore, these loads were excluded from analysis. A trend (p = 0.07) for a main effect of load existed for the leg press exercise, indicating that the 40% MIF load tended to elicit greater power output than the 60% MIF load (effect size = 0.38). A significant (p . 0.05) main effect of load existed for the bench press exercise; post hoc analysis indicated that the effect of load on power output was: 30% > 40% > 50% = 60%. CONCLUSION: Loads of 40% and 30% of MIF elicit maximal power output during dynamic leg presses and bench presses, respectively. These findings are similar to those obtained when loading is based on 1-RM.

President's Assembly--State Policy Research at the University of Illinois. Final Report.

ERIC Educational Resources Information Center

Gove, Samuel K., Ed.; Zollinger, Richard A., Ed.

The activities at the President's Assembly on State Policy Research at the University of Illinois, which was convened to explore a series of questions related to the interaction between universities and the agencies that create public policy, are reported. The report of the assembly, which is a statement representing general agreement among the…

75 FR 39729 - Departmental Offices; Debt Management Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-12

... final decisions on major financing operations. Historically, this advice has been offered by debt.... Although the Treasury's final announcement of financing plans may not reflect the recommendations provided... economic conditions and financing estimates. This briefing will give the press an opportunity to ask...

Enery Efficient Press and Sinter of Titanium Powder for Low-Cost Components in Vehicle Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thomas Zwitter; Phillip Nash; Xiaoyan Xu

2011-03-31

This is the final technical report for the Department of Energy NETL project NT01931 Energy Efficient Press and Sinter of Titanium Powder for Low-Cost Components in Vehicle Applications. Titanium has been identified as one of the key materials with the required strength that can reduce the weight of automotive components and thereby reduce fuel consumption. Working with newly developed sources of titanium powder, Webster-Hoff will develop the processing technology to manufacture low cost vehicle components using the single press/single sinter techniques developed for iron based powder metallurgy today. Working with an automotive or truck manufacturer, Webster-Hoff will demonstrate the feasibilitymore » of manufacturing a press and sinter titanium component for a vehicle application. The project objective is two-fold, to develop the technology for manufacturing press and sinter titanium components, and to demonstrate the feasibility of producing a titanium component for a vehicle application. The lowest cost method for converting metal powder into a net shape part is the Powder Metallurgy Press and Sinter Process. The method involves compaction of the metal powder in a tool (usually a die and punches, upper and lower) at a high pressure (up to 60 TSI or 827 MPa) to form a green compact with the net shape of the final component. The powder in the green compact is held together by the compression bonds between the powder particles. The sinter process then converts the green compact to a metallurgically bonded net shape part through the process of solid state diffusion. The goal of this project is to expand the understanding and application of press and sinter technology to Titanium Powder applications, developing techniques to manufacture net shape Titanium components via the press and sinter process. In addition, working with a vehicle manufacturer, demonstrate the feasibility of producing a titanium component for a vehicle. This is not a research program, but rather a project to develop a process for press and sinter of net shape Titanium components. All of these project objectives have been successfully completed.« less

Proteogenomics produces comprehensive and highly accurate protein-coding gene annotation in a complete genome assembly of Malassezia sympodialis.

PubMed

Zhu, Yafeng; Engström, Pär G; Tellgren-Roth, Christian; Baudo, Charles D; Kennell, John C; Sun, Sheng; Billmyre, R Blake; Schröder, Markus S; Andersson, Anna; Holm, Tina; Sigurgeirsson, Benjamin; Wu, Guangxi; Sankaranarayanan, Sundar Ram; Siddharthan, Rahul; Sanyal, Kaustuv; Lundeberg, Joakim; Nystedt, Björn; Boekhout, Teun; Dawson, Thomas L; Heitman, Joseph; Scheynius, Annika; Lehtiö, Janne

2017-03-17

Complete and accurate genome assembly and annotation is a crucial foundation for comparative and functional genomics. Despite this, few complete eukaryotic genomes are available, and genome annotation remains a major challenge. Here, we present a complete genome assembly of the skin commensal yeast Malassezia sympodialis and demonstrate how proteogenomics can substantially improve gene annotation. Through long-read DNA sequencing, we obtained a gap-free genome assembly for M. sympodialis (ATCC 42132), comprising eight nuclear and one mitochondrial chromosome. We also sequenced and assembled four M. sympodialis clinical isolates, and showed their value for understanding Malassezia reproduction by confirming four alternative allele combinations at the two mating-type loci. Importantly, we demonstrated how proteomics data could be readily integrated with transcriptomics data in standard annotation tools. This increased the number of annotated protein-coding genes by 14% (from 3612 to 4113), compared to using transcriptomics evidence alone. Manual curation further increased the number of protein-coding genes by 9% (to 4493). All of these genes have RNA-seq evidence and 87% were confirmed by proteomics. The M. sympodialis genome assembly and annotation presented here is at a quality yet achieved only for a few eukaryotic organisms, and constitutes an important reference for future host-microbe interaction studies. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Expedition 38 Press Conference

NASA Image and Video Library

2013-11-06

Expedition 38 Flight Engineer Rick Mastracchio of NASA talks, while in quarantine behind glass, during the final press conference held a day ahead of his launch with fellow crew mates, Soyuz Commander Mikhail Tyurin of Roscosmos, and, Flight Engineer Koichi Wakata of the Japan Aerospace Exploration Agency, to the International Space Station, Wednesday, Nov. 6, 2013 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Bill Ingalls)

Expedition 38 Press Conference

NASA Image and Video Library

2013-11-06

Expedition 38 Flight Engineer Koichi Wakata of the Japan Aerospace Exploration Agency is seen in quarantine, behind glass, during the final press conference held a day ahead of his launch with fellow crew mates, Expedition 38 Soyuz Commander Mikhail Tyurin of Roscosmos, and, Flight Engineer Rick Mastracchio of NASA, to the International Space Station, Wednesday, Nov. 6, 2013 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Bill Ingalls)

Expedition 38 Press Conference

NASA Image and Video Library

2013-11-06

Expedition 38 Soyuz Commander Mikhail Tyurin of Roscosmos is seen in quarantine behind glass during the final press conference held a day ahead of his launch with fellow crew mates, Flight Engineer Koichi Wakata of the Japan Aerospace Exploration Agency, and, Flight Engineer Rick Mastracchio of NASA, to the International Space Station, Wednesday, Nov. 6, 2013 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Bill Ingalls)

Expedition 38 Press Conference

NASA Image and Video Library

2013-11-06

Expedition 38 Soyuz Commander Mikhail Tyurin of Roscosmos, right, talks as Flight Engineer Koichi Wakata of the Japan Aerospace Exploration Agency, listens, from quarantine behind glass, during the final press conference held a day ahead of their launch with fellow crew mate, Flight Engineer Rick Mastracchio of NASA, to the International Space Station, Wednesday, Nov. 6, 2013 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Bill Ingalls)

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 flight engineer Steve Swanson of NASA, Soyuz commander Aleksander Skvortsov of the Russian Federal Space Agency, Roscosmos, and flight engineer Oleg Artemyev of Roscosmos are seen in quarantine, behind glass, during the final press conference be Monday, March 24, 2014, at the Cosmonaut Hotel in Baikonur, Kazakhstan. The mission to the International Space Station is set to launch March 26 from the Baikonur Cosmodrome. Photo Credit: (NASA/Joel Kowsky)

Characterizing the Elastic Behaviour of a Press Table through Topology Optimization

NASA Astrophysics Data System (ADS)

Pilthammar, J.; Sigvant, M.; Hansson, M.; Pálsson, E.; Rutgersson, W.

2017-09-01

Sheet metal forming in the car industry is a highly competitive area. The use of digital techniques and numerical methods are therefore of high interest for reduced costs and lead times. One method for reducing the try-out phase is virtual rework of die surfaces. The virtual rework is based on Finite Element (FE) simulations and can reduce and support manual rework. The elastic behaviour of dies and presses must be represented in a reliable way in FE-models to be able to perform virtual rework. CAD-models exists for nearly all dies today, but not for press lines. A full geometrical representation of presses will also yield very large FE- models. This paper will discuss and demonstrate a strategy for measuring and characterizing a press table for inclusion in FE-models. The measurements of the elastic press deformations is carried out with force transducers and an ARAMIS 3D optical measurement system. The press table is then inverse modelled by topology optimization using the recorded results as boundary conditions. Finally, the press table is coupled with a FE-model of a die to demonstrate its influence on the deformations. This indicates the importance of having a reliable representation of the press deformations during virtual rework.

Finalizing the DTAG: Implementation and Testing of Design Improvements for Reliability and Availability

DTIC Science & Technology

2015-09-30

acoustics and fine scale motion. The success of the Dtag has resulted in an increased demand for the instrument from researchers both within the...sensor blocks sound when the animal is close to the surface. The polyethylene shell was eliminated in the Dtag-3 design to improve acoustic ...into 3 main sub-assemblies (Figure 5): 1) foam sub-assembly, 2) sensor sub-assembly, and 3) Electronics sub-assembly. This separation enables rapid

Directing folding pathways for multi-component DNA origami nanostructures with complex topology

NASA Astrophysics Data System (ADS)

Marras, A. E.; Zhou, L.; Kolliopoulos, V.; Su, H.-J.; Castro, C. E.

2016-05-01

Molecular self-assembly has become a well-established technique to design complex nanostructures and hierarchical mesoscale assemblies. The typical approach is to design binding complementarity into nucleotide or amino acid sequences to achieve the desired final geometry. However, with an increasing interest in dynamic nanodevices, the need to design structures with motion has necessitated the development of multi-component structures. While this has been achieved through hierarchical assembly of similar structural units, here we focus on the assembly of topologically complex structures, specifically with concentric components, where post-folding assembly is not feasible. We exploit the ability to direct folding pathways to program the sequence of assembly and present a novel approach of designing the strand topology of intermediate folding states to program the topology of the final structure, in this case a DNA origami slider structure that functions much like a piston-cylinder assembly in an engine. The ability to program the sequence and control orientation and topology of multi-component DNA origami nanostructures provides a foundation for a new class of structures with internal and external moving parts and complex scaffold topology. Furthermore, this work provides critical insight to guide the design of intermediate states along a DNA origami folding pathway and to further understand the details of DNA origami self-assembly to more broadly control folding states and landscapes.

A resolution condemning the Government of Iran for restricting and suppressing freedom of the press, freedom of speech, freedom of expression, and freedom of assembly, and for its human rights abuses, and for other purposes.

THOMAS, 111th Congress

Sen. Kaufman, Edward E. [D-DE

2009-12-22

Senate - 12/22/2009 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (All Actions) Tracker: This bill has the status Agreed to in SenateHere are the steps for Status of Legislation:

KSC-2013-2761

NASA Image and Video Library

2013-06-14

CAPE CANAVERAL, Fla. – At the Kennedy Space Center in Florida, a fitness trail is under construction in the Launch Complex 39 area across from the Vehicle Assembly Building and just west of the Press Site. This panoramic image shows that that trail is being designed in the shape of a space shuttle. The trail will provide an opportunity for employees at the spaceport to stay physically fit. Photo credit: NASA/Jim Grossmann

US-Korea Nanotechnology, Biotechnology, and Information Technology (NBIT) Symbiosys Program - Phase 1

DTIC Science & Technology

2010-10-01

diameter, length, and spacing. Fabrication Technology: Synthesis of self-organized AAO ( Anodic aluminum oxide ) templates with controlled diameter...nanowires arrayed in anodized aluminum oxide ( AAO ) templates and the diameter is precisely controlled by using atomic layer deposition (ALD) process...Jin, “Highly Self-assembled Nanotubular Aluminum Oxide by Hard Anodization ”, J. Mater. Res. (in press, December 2010). 3. J.Y. Kim, K. Noh, C. Choi

Industrial robots: Handbook

NASA Astrophysics Data System (ADS)

Kozyrev, Iu. G.

Topics covered include terms, definitions, and classification; operator-directed manipulators; autooperators as used in automated pressure casting; construction and application of industrial robots; and the operating bases of automated systems. Attention is given to adaptive and interactive robots; gripping mechanisms; applications to foundary production, press-forging plants, heat treatment, welding, and assembly operations. A review of design recommendations includes a determination of fundamental structural and technological indicators for industrial robots and a consideration of drive mechanisms.

Method of preparing an electrochemical cell in uncharged state

DOEpatents

Shimotake, Hiroshi; Bartholme, Louis G.; Arntzen, John D.

1977-02-01

A secondary electrochemical cell is assembled in an uncharged state for the preparation of a lithium alloy-transition metal sulfide cell. The negative electrode includes a material such as aluminum or silicon for alloying with lithium as the cell is charged. The positive electrode is prepared by blending particulate lithium sulfide, transition metal powder and electrolytic salt in solid phase. The mixture is simultaneously heated to a temperature in excess of the melting point of the electrolyte and pressed onto an electrically conductive substrate to form a plaque. The plaque is assembled as a positive electrode within the cell. During the first charge cycle lithium alloy is formed within the negative electrode and transition metal sulfide such as iron sulfide is produced within the positive electrode.

Fabrication of circular assemblies with DNA tetrahedrons: from static structures to a dynamic rotary motor.

PubMed

Wang, Liying; Meng, Zhenyu; Martina, Felicia; Shao, Huilin; Shao, Fangwei

2017-12-01

DNA tetrahedron as the simplest 3D DNA nanostructure has been applied widely in biomedicine and biosensing. Herein, we design and fabricate a series of circular assemblies of DNA tetrahedron with high purity and decent yields. These circular nanostructures are confirmed by endonuclease digestion, gel electrophoresis and atomic force microscopy. Inspired by rotary protein motor, we demonstrate these circular architectures can serve as a stator for a rotary DNA motor to achieve the circular rotation. The DNA motor can rotate on the stators for several cycles, and the locomotion of the motor is monitored by the real-time fluorescent measurements. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Long-term nitrogen addition affects the phylogenetic turnover of soil microbial community responding to moisture pulse

DOE PAGES

Liu, Chi; Yao, Minjie; Stegen, James C.; ...

2017-12-13

How press disturbance (long-term) influences the phylogenetic turnover of soil microbial communities responding to pulse disturbances (short-term) is not fully known. Understanding the complex connections between the history of environmental conditions, assembly processes and microbial community dynamics is necessary to predict microbial response to perturbation. Here, we started by investigating phylogenetic spatial turnover (based on DNA) of soil prokaryotic communities after long-term nitrogen (N) deposition and temporal turnover (based on RNA) of communities responding to pulse by conducting short-term rewetting experiments. The results showed that moderate N addition increased ecological stochasticity and phylogenetic diversity. In contrast, high N addition slightlymore » increased homogeneous selection and decreased phylogenetic diversity. Examining the system with higher phylogenetic resolution revealed a moderate contribution of variable selection across the whole N gradient. The moisture pulse experiment showed that high N soils had higher rates of phylogenetic turnover across short phylogenetic distances and significant changes in community compositions through time. Long-term N input history influenced spatial turnover of microbial communities, but the dominant community assembly mechanisms differed across different N deposition gradients. We further revealed an interaction between press and pulse disturbances whereby deterministic processes were particularly important following pulse disturbances in high N soils.« less

Long-term nitrogen addition affects the phylogenetic turnover of soil microbial community responding to moisture pulse

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Chi; Yao, Minjie; Stegen, James C.

How press disturbance (long-term) influences the phylogenetic turnover of soil microbial communities responding to pulse disturbances (short-term) is not fully known. Understanding the complex connections between the history of environmental conditions, assembly processes and microbial community dynamics is necessary to predict microbial response to perturbation. Here, we started by investigating phylogenetic spatial turnover (based on DNA) of soil prokaryotic communities after long-term nitrogen (N) deposition and temporal turnover (based on RNA) of communities responding to pulse by conducting short-term rewetting experiments. The results showed that moderate N addition increased ecological stochasticity and phylogenetic diversity. In contrast, high N addition slightlymore » increased homogeneous selection and decreased phylogenetic diversity. Examining the system with higher phylogenetic resolution revealed a moderate contribution of variable selection across the whole N gradient. The moisture pulse experiment showed that high N soils had higher rates of phylogenetic turnover across short phylogenetic distances and significant changes in community compositions through time. Long-term N input history influenced spatial turnover of microbial communities, but the dominant community assembly mechanisms differed across different N deposition gradients. We further revealed an interaction between press and pulse disturbances whereby deterministic processes were particularly important following pulse disturbances in high N soils.« less

Long-term nitrogen addition affects the phylogenetic turnover of soil microbial community responding to moisture pulse.

PubMed

Liu, Chi; Yao, Minjie; Stegen, James C; Rui, Junpeng; Li, Jiabao; Li, Xiangzhen

2017-12-13

How press disturbance (long-term) influences the phylogenetic turnover of soil microbial communities responding to pulse disturbances (short-term) is not fully known. Understanding the complex connections between the history of environmental conditions, assembly processes and microbial community dynamics is necessary to predict microbial response to perturbation. We started by investigating phylogenetic spatial turnover (based on DNA) of soil prokaryotic communities after long-term nitrogen (N) deposition and temporal turnover (based on RNA) of communities responding to pulse by conducting short-term rewetting experiments. The results showed that moderate N addition increased ecological stochasticity and phylogenetic diversity. In contrast, high N addition slightly increased homogeneous selection and decreased phylogenetic diversity. Examining the system with higher phylogenetic resolution revealed a moderate contribution of variable selection across the whole N gradient. The moisture pulse experiment showed that high N soils had higher rates of phylogenetic turnover across short phylogenetic distances and significant changes in community compositions through time. Long-term N input history influenced spatial turnover of microbial communities, but the dominant community assembly mechanisms differed across different N deposition gradients. We further revealed an interaction between press and pulse disturbances whereby deterministic processes were particularly important following pulse disturbances in high N soils.

Phosphoric and electric utility fuel technology development

NASA Astrophysics Data System (ADS)

Breault, R. D.; Briggs, T. A.; Congdon, J. V.; Gelting, R. L.; Goller, G. J.; Luoma, W. L.; McCloskey, M. W.; Mientek, A. P.; Obrien, J. J.; Randall, S. A.

1985-05-01

Seventeen hundred hours and 11 thermal cycles were accumulated on the second 10 sq ft short stack at 120 psia and 405 F. A subscale cell out from 10 sq ft electrodes in the same batch used for the second 10 sq ft short stack accumulated over 4100 hours with performance conforming close to the E-line goal at 120 psia and 400 F. Over 14,870 hours and 42 thermal cycles were accumulated on the 3.7 sq ft, 30-cell short stack at 120 psia and 405 F. A subscale cell with GSB-18 catalyst completed over 10,000 hours of operation at 120 psia, 400 F. The full-size, 10 sq ft stack containment vessel and reactant gas manifolds were observed. The improved edge seal decreased leakage by more than 50% from the conventional edge seal. Cross-pressure tolerance also improved. Continuous automatic operation of the substrate forming machine was demonstrated by producing substrates at a 50% faster rate with high yields and low material loss. The cooler bonding cycle was significantly reduced by using a cold press in conjunction with the hot press. A lower cost stainless steel tubing is identified that could reduce cooler array cost by up to 50%. Assembly of the automated cell fill and assembly machine is initiated.

KENNEDY SPACE CENTER, FLA. -- A breeding osprey occupies a nest constructed on a speaker pole in the lower parking lot of the KSC Press Site. Eggs have been sighted in the nest. The NASA logo in the background is painted on an outer wall of the 525-foot-tall Vehicle Assembly Building nearby. Known as a fish hawk, the osprey selects sites of opportunity in which to nest -- from trees and telephone poles to rocks or even flat ground. In North America, it is found from Alaska and Newfoundland to Florida and the Gulf Coast. Osprey nests are found throughout the Kennedy Space Center and surrounding Merritt Island National Wildlife Refuge.

NASA Image and Video Library

2004-01-14

KENNEDY SPACE CENTER, FLA. -- A breeding osprey occupies a nest constructed on a speaker pole in the lower parking lot of the KSC Press Site. Eggs have been sighted in the nest. The NASA logo in the background is painted on an outer wall of the 525-foot-tall Vehicle Assembly Building nearby. Known as a fish hawk, the osprey selects sites of opportunity in which to nest -- from trees and telephone poles to rocks or even flat ground. In North America, it is found from Alaska and Newfoundland to Florida and the Gulf Coast. Osprey nests are found throughout the Kennedy Space Center and surrounding Merritt Island National Wildlife Refuge.

KENNEDY SPACE CENTER, FLA. -- A pair of breeding ospreys share a nest constructed on a speaker pole in the lower parking lot of the KSC Press Site. Eggs have been sighted in the nest. The NASA logo in the background is painted on an outer wall of the 525-foot-tall Vehicle Assembly Building nearby. Known as a fish hawk, the osprey selects sites of opportunity in which to nest -- from trees and telephone poles to rocks or even flat ground. In North America, it is found from Alaska and Newfoundland to Florida and the Gulf Coast. Osprey nests are found throughout the Kennedy Space Center and surrounding Merritt Island National Wildlife Refuge.

NASA Image and Video Library

2004-01-14

KENNEDY SPACE CENTER, FLA. -- A pair of breeding ospreys share a nest constructed on a speaker pole in the lower parking lot of the KSC Press Site. Eggs have been sighted in the nest. The NASA logo in the background is painted on an outer wall of the 525-foot-tall Vehicle Assembly Building nearby. Known as a fish hawk, the osprey selects sites of opportunity in which to nest -- from trees and telephone poles to rocks or even flat ground. In North America, it is found from Alaska and Newfoundland to Florida and the Gulf Coast. Osprey nests are found throughout the Kennedy Space Center and surrounding Merritt Island National Wildlife Refuge.

76 FR 20819 - Departmental Offices; Debt Management Advisory Committee Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-13

... prior to making its final decisions on major financing operations. Historically, this advice has been.... 2, Sec. 3. Although the Treasury's final announcement of financing plans may not reflect the... statement of economic conditions and financing estimates. This briefing will give the press an opportunity...

de novo assembly and population genomic survey of natural yeast isolates with the Oxford Nanopore MinION sequencer.

PubMed

Istace, Benjamin; Friedrich, Anne; d'Agata, Léo; Faye, Sébastien; Payen, Emilie; Beluche, Odette; Caradec, Claudia; Davidas, Sabrina; Cruaud, Corinne; Liti, Gianni; Lemainque, Arnaud; Engelen, Stefan; Wincker, Patrick; Schacherer, Joseph; Aury, Jean-Marc

2017-02-01

Oxford Nanopore Technologies Ltd (Oxford, UK) have recently commercialized MinION, a small single-molecule nanopore sequencer, that offers the possibility of sequencing long DNA fragments from small genomes in a matter of seconds. The Oxford Nanopore technology is truly disruptive; it has the potential to revolutionize genomic applications due to its portability, low cost, and ease of use compared with existing long reads sequencing technologies. The MinION sequencer enables the rapid sequencing of small eukaryotic genomes, such as the yeast genome. Combined with existing assembler algorithms, near complete genome assemblies can be generated and comprehensive population genomic analyses can be performed. Here, we resequenced the genome of the Saccharomyces cerevisiae S288C strain to evaluate the performance of nanopore-only assemblers. Then we de novo sequenced and assembled the genomes of 21 isolates representative of the S. cerevisiae genetic diversity using the MinION platform. The contiguity of our assemblies was 14 times higher than the Illumina-only assemblies and we obtained one or two long contigs for 65 % of the chromosomes. This high contiguity allowed us to accurately detect large structural variations across the 21 studied genomes. Because of the high completeness of the nanopore assemblies, we were able to produce a complete cartography of transposable elements insertions and inspect structural variants that are generally missed using a short-read sequencing strategy. Our analyses show that the Oxford Nanopore technology is already usable for de novo sequencing and assembly; however, non-random errors in homopolymers require polishing the consensus using an alternate sequencing technology. © The Author 2017. Published by Oxford University Press.

Temporal Progression of Visual Injury from Blast Exposure

DTIC Science & Technology

2013-09-01

included the design and construction of a silencer and dump tank. The final design is shown in Figure 8. A steel barrel lined with 2” of acoustic foam...was selected as the dump tank. It surrounds a rubber barrel lined with foam composite. The steel barrel is allowed to recoil on a cart, absorbing...test. Figure 8. (Left) Inner silencer assembly completed during Q4 of Year 1. (Right) Final silencer assembly with the outer steel drum

Qualification of the gritblast assembly and process for the inside diameter of the RSRM forward and aft domes

NASA Technical Reports Server (NTRS)

Nolan, Michael J.

1992-01-01

This gritblast assembly shall be used when refurbishing the Inside Diameter (ID) of RSRM forward and aft domes. Initial blasting is used to remove corrosion and adhesive not removed during the insulation washout. Final blasting is conducted just prior to part finalization in order to remove residual contamination and prepare the ID surface for bonding. The media used in this gritblaster is DuPont Zirclean Blasting Abrasive. It is possible to use other media in this gritblast assembly, however the only facility that has the control capability for this assembly is the Zirclean blast booth. This blast booth can not use other media without the occurrence of contamination. This automated process shall replace the manually controlled gritblasting that is currently in operation. Manual gritblasting does not provide the consistency, control, and safety that an automated process is capable of delivering.

Genome assembly and transcriptome resource for river buffalo, Bubalus bubalis (2n = 50).

PubMed

Williams, John L; Iamartino, Daniela; Pruitt, Kim D; Sonstegard, Tad; Smith, Timothy P L; Low, Wai Yee; Biagini, Tommaso; Bomba, Lorenzo; Capomaccio, Stefano; Castiglioni, Bianca; Coletta, Angelo; Corrado, Federica; Ferré, Fabrizio; Iannuzzi, Leopoldo; Lawley, Cynthia; Macciotta, Nicolò; McClure, Matthew; Mancini, Giordano; Matassino, Donato; Mazza, Raffaele; Milanesi, Marco; Moioli, Bianca; Morandi, Nicola; Ramunno, Luigi; Peretti, Vincenzo; Pilla, Fabio; Ramelli, Paola; Schroeder, Steven; Strozzi, Francesco; Thibaud-Nissen, Francoise; Zicarelli, Luigi; Ajmone-Marsan, Paolo; Valentini, Alessio; Chillemi, Giovanni; Zimin, Aleksey

2017-10-01

Water buffalo is a globally important species for agriculture and local economies. A de novo assembled, well-annotated reference sequence for the water buffalo is an important prerequisite for studying the biology of this species, and is necessary to manage genetic diversity and to use modern breeding and genomic selection techniques. However, no such genome assembly has been previously reported. There are 2 species of domestic water buffalo, the river (2 n = 50) and the swamp (2 n = 48) buffalo. Here we describe a draft quality reference sequence for the river buffalo created from Illumina GA and Roche 454 short read sequences using the MaSuRCA assembler. The assembled sequence is 2.83 Gb, consisting of 366 983 scaffolds with a scaffold N50 of 1.41 Mb and contig N50 of 21 398 bp. Annotation of the genome was supported by transcriptome data from 30 tissues and identified 21 711 predicted protein coding genes. Searches for complete mammalian BUSCO gene groups found 98.6% of curated single copy orthologs present among predicted genes, which suggests a high level of completeness of the genome. The annotated sequence is available from NCBI at accession GCA_000471725.1. © The Author 2017. Published by Oxford University Press.

Enhanced Thermal Transport of Surfaces with Superhydrophobic Coatings

DTIC Science & Technology

2015-07-01

transport, superhydrophobic, jumping droplet, cooling, nanostructure, self - assembled monolayer 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF...modified from a hydrophilic chemistry (oxide) to a hydrophobic surface using a fluorinated (or protonated) self - assembled monolayer (SAM). Chemical...seconds and dried with filtered nitrogen. 2.3 SAM Deposition The final step involved the deposition of a self - assembled monolayer onto the silvered

KSC-2010-4888

NASA Image and Video Library

2010-09-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, Canadian Space Agency astronaut Chris Hadfield address the attendees at a ceremony being held to commemorate the move from Kennedy's Assembly Refurbishment Facility (ARF) to the Vehicle Assembly Building (VAB) of the Space Shuttle Program's final solid rocket booster structural assembly -- the right-hand forward. The move was postponed because of inclement weather. Photo credit: NASA/Kim Shiflett

KSC-2010-4886

NASA Image and Video Library

2010-09-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, Roger Elliot with United Space Alliance addresses the attendees at a ceremony being held to commemorate the move from Kennedy's Assembly Refurbishment Facility (ARF) to the Vehicle Assembly Building (VAB) of the Space Shuttle Program's final solid rocket booster structural assembly -- the right-hand forward. The move was postponed because of inclement weather. Photo credit: NASA/Kim Shiflett

KSC-2010-4887

NASA Image and Video Library

2010-09-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, Center Director Bob Cabana speaks to the attendees at a ceremony being held to commemorate the move from Kennedy's Assembly Refurbishment Facility (ARF) to the Vehicle Assembly Building (VAB) of the Space Shuttle Program's final solid rocket booster structural assembly -- the right-hand forward. The move was postponed because of inclement weather. Photo credit: NASA/Kim Shiflett

Design and Fabrication of Cryostat Interface and Electronics for High Performance Antimatter Trap (HI-PAT)

NASA Technical Reports Server (NTRS)

Smith, Gerald A.

1999-01-01

Included in Appendix I to this report is a complete set of design and assembly schematics for the high vacuum inner trap assembly, cryostat interfaces and electronic components for the MSFC HI-PAT. Also included in the final report are summaries of vacuum tests, and electronic tests performed upon completion of the assembly.

Damper mechanism for nuclear reactor control elements

DOEpatents

Taft, William Elwood

1976-01-01

A damper mechanism which provides a nuclear reactor control element decelerating function at the end of the scram stroke. The total damping function is produced by the combination of two assemblies, which operate in sequence. First, a tapered dashram assembly decelerates the control element to a lower velocity, after which a spring hydraulic damper assembly takes over to complete the final damping.

Rotating mandrel speeds assembly of plastic inflatables

NASA Technical Reports Server (NTRS)

Mac Fadden, J. A.; Stenlund, S. J.; Wendt, A. J.

1966-01-01

Rotating mandrel permits the accurate cutting, forming, and sealing of plastic gores for assembly of an inflatable surface of revolution. The gores remain on the mandrel until the final seam is reached. Tolerances are tightly controlled by the mandrel configuration.

Aerobrake assembly with minimum Space Station accommodation

NASA Technical Reports Server (NTRS)

Katzberg, Steven J.; Butler, David H.; Doggett, William R.; Russell, James W.; Hurban, Theresa

1991-01-01

The minimum Space Station Freedom accommodations required for initial assembly, repair, and refurbishment of the Lunar aerobrake were investigated. Baseline Space Station Freedom support services were assumed, as well as reasonable earth-to-orbit possibilities. A set of three aerobrake configurations representative of the major themes in aerobraking were developed. Structural assembly concepts, along with on-orbit assembly and refurbishment scenarios were created. The scenarios were exercised to identify required Space Station Freedom accommodations. Finally, important areas for follow-on study were also identified.

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 primary crew flight engineer Steve Swanson of NASA, Soyuz commander Aleksander Skvortsov of the Russian Federal Space Agency, Roscosmos, flight engineer Oleg Artemyev of Roscosmos are seen in quarantine, behind glass, during the final press conference be Monday, March 24, 2014, at the Cosmonaut Hotel in Baikonur, Kazakhstan. The mission to the International Space Station is set to launch March 26 from the Baikonur Cosmodrome. Photo Credit: (NASA/Joel Kowsky)

expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 primary crew flight engineer Steve Swanson of NASA, Soyuz commander Aleksander Skvortsov of the Russian Federal Space Agency, Roscosmos, flight engineer Oleg Artemyev of Roscosmos are seen in quarantine, behind glass, during the final press conference be Monday, March 24, 2014, at the Cosmonaut Hotel in Baikonur, Kazakhstan. The mission to the International Space Station is set to launch March 26 from the Baikonur Cosmodrome. Photo Credit: (NASA/Joel Kowsky)

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 backup crew member Barry Wilmore of NASA is seen in quarantine, behind glass, during the final press conference held ahead of the launch of Expedition 39 prime crew members; Soyuz Commander Alexander Skvortsov of the Russian Federal Space Agency, Flight Engineer Steve Swanson of NASA, and Flight Engineer Oleg Artemyev of Roscosmos, to the International Space Station, Monday, March 24, 2014 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Joel Kowsky)

STS_135_ MEDIA

NASA Image and Video Library

2011-06-30

JSC2011-E-060797 (30 June 2011) --- Commander Chris Ferguson, pilot Doug Hurley, mission specialist Sandy Magnus and mission specialist Rex Walheim participate in the STS-135 crew media briefing at NASA?s Johnson Space Center June 30, 2011. The press conference provided the last scheduled opportunity for a large group of press to speak with the crew before the final launch on July 8. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

JPL-20170915-CASSINf-0002-Cassini End of Mission Post Event Press ConferenceAVAIL

NASA Image and Video Library

2017-09-15

This press briefing summarizes the end of NASA-ESA's Cassini-Huygens mission to Saturn and presents the final images made by the spacecraft before its planned disintegration in Saturn's atmosphere on September 15, 2017. Featured: Earl Maize, Cassini Program Manager, JPL; Linda Spilker, Cassini Project Scientist, JPL; Julie Webster, Cassini Spacecraft operations Manager, JPL; and Thomas Zurbuchen, Associate Administrator, Science Mission Directorate, NASA HQ.

Membrane-electrode assemblies for electrochemical cells

DOEpatents

Swathirajan, Sundararajan; Mikhail, Youssef M.

1993-01-01

A combination, unitary, membrane and electrode assembly with a solid polymer electrolyte membrane, and first and second electrodes at least partially embedded in opposed surfaces of the membrane. The electrodes each comprise a respective group of finely divided carbon particles, very finely divided catalytic particles supported on internal and external surfaces of the carbon particles and a proton conductive material intermingled with the catalytic and carbon particles. A first group of finely divided carbon particles forming the first electrode has greater water attraction and retention properties, and is more hydrophilic than a second group of carbon particles forming the second electrode. In a preferred method, the membrane electrode assembly of the invention is prepared by forming a slurry of proton conductive material and at least one group of the carbon and catalyst particles. The slurry is applied to the opposed surfaces of the membrane and heated while being pressed to the membrane for a time and at a temperature and compressive load sufficient to embed at least a portion of the particles into the membrane.

Molecular Structure of a Helical ribbon in a Peptide Self-Assembly

NASA Astrophysics Data System (ADS)

Hwang, Wonmuk; Marini, Davide; Kamm, Roger D.; Zhang, Shuguang

2002-03-01

We have studied the molecular structure of nanometer scale helical ribbons observed during self-assembly of the peptide KFE8 (amino acid sequence: FKFEFKFE) (NanoLetters (2002, in press)). By analyzing the hydrogen bonding patterns between neighboring peptide backbones, we constructed a number of possible β-sheets. Using all possible combinations of these, we built helical ribbons with dimensions close to those found experimentally and performed molecular dynamics simulations to identify the most stable structure. Solvation effects were implemented by the analytic continuum electrostatics (ACE) model developed by Schaefer and Karplus (J. Phys. Chem. 100, 1578 (1996)). By applying electrostatic double layer theory, we incorporated the effect of pH by scaling the amount of charge on the sidechains. Our results suggest that the helical ribbon is comprised of a double β-sheet where the inner and the outer helices have distinct hydrogen bonding patterns. Our approach has general applicability to the study of helices formed by the self-assembly of β-sheet forming peptides with various amino acid sequences.

Fully packed capillary electrochromatographic microchip with self-assembly colloidal silica beads.

PubMed

Park, Jongman; Lee, Dami; Kim, Won; Horiike, Shigeyoshi; Nishimoto, Takahiro; Lee, Se Hwan; Ahn, Chong H

2007-04-15

A fully packed capillary electrochromatographic (CEC) microchip showing improved solution and chip handling was developed. Microchannels for the CEC microchip were patterned on a cyclic olefin copolymer substrate by injection molding and packed fully with 0.8-microm monodisperse colloidal silica beads utilizing a self-assembly packing technique. The silica packed chip substrate was covered and thermally press-bonded. After fabrication, the chip was filled with buffer solution by self-priming capillary action. The self-assembly packing at each channel served as a built-in nanofilter allowing quick loading of samples and running buffer solution without filtration. Because of a large surface area-to-volume ratio of the silica packing, reproducible control of electroosmotic flow was possible without leveling of the solutions in the reservoirs resulting 1.3% rsd in migration rate. The capillary electrophoretic separation characteristics of the chip were studied using fluorescein isothiocyanate (FITC)-derivatized amino acids as probe molecules. A mixture of FITC and four FITC-derivatized amino acids was successfully separated with 2-mm separation channel length.

Real-Time Probing of Nanowire Assembly Kinetics at the Air-Water Interface by In Situ Synchrotron X-Ray Scattering.

PubMed

He, Zhen; Jiang, Hui-Jun; Wu, Long-Long; Liu, Jian-Wei; Wang, Geng; Wang, Xiao; Wang, Jin-Long; Hou, Zhong-Huai; Chen, Gang; Yu, Shu-Hong

2018-07-02

Although many assembly strategies have been used to successfully construct well-aligned nanowire (NW) assemblies, the understanding of their assembly kinetics has remained elusive, which restricts the development of NW-based device and circuit fabrication. Now a versatile strategy that combines interfacial assembly and synchrotron-based grazing-incidence small-angle X-ray scattering (GISAXS) is presented to track the assembly evolution of the NWs in real time. During the interface assembly process, the randomly dispersed NWs gradually aggregate to form small ordered NW-blocks and finally are constructed into well-defined NW monolayer driven by the conformation entropy. The NW assembly mechanism can be well revealed by the thermodynamic analysis and large-scale molecular dynamics theoretical evaluation. These findings point to new opportunities for understanding NW assembly kinetics and manipulating NW assembled structures by bottom-up strategy. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Kennedy Space Center Press Site (SWMU 074) Interim Measure Report

NASA Technical Reports Server (NTRS)

Applegate, Joseph L.

2015-01-01

This report summarizes the Interim Measure (IM) activities conducted at the Kennedy Space Center (KSC) Press Site ("the Press Site"). This facility has been designated as Solid Waste Management Unit 074 under KSC's Resource Conservation and Recovery Act Corrective Action program. The activities were completed as part of the Vehicle Assembly Building (VAB) Area Land Use Controls Implementation Plan (LUCIP) Elimination Project. The purpose of the VAB Area LUCIP Elimination Project was to delineate and remove soil affected with constituents of concern (COCs) that historically resulted in Land Use Controls (LUCs). The goal of the project was to eliminate the LUCs on soil. LUCs for groundwater were not addressed as part of the project and are not discussed in this report. This report is intended to meet the Florida Department of Environmental Protection (FDEP) Corrective Action Management Plan requirement as part of the KSC Hazardous and Solid Waste Amendments permit and the U.S. Environmental Protection Agency's (USEPA's) Toxic Substance Control Act (TSCA) self-implementing polychlorinated biphenyl (PCB) cleanup requirements of 40 Code of Federal Regulations (CFR) 761.61(a).

Fabrication of micro metallic valve and pump

NASA Astrophysics Data System (ADS)

Yang, Ming; Kabasawa, Yasunari; Ito, Kuniyoshi

2010-03-01

Fabrication of micro devices by using micro metal forming was proposed by the authors. We developed a desktop servo-press machine with precise tooling system. Precise press forming processes including micro forging and micro joining has been carried out in a progressive die. In this study, micro metallic valve and pump were fabricated by using the precise press forming. The components are made of sheet metals, and assembled in to a unit in the progressive die. A micro check-valve with a diameter of 3mm and a length of 3.2mm was fabricated, and the property of flow resistance was evaluated. The results show that the check valve has high property of leakage proof. Since the valve is a unit parts with dimensions of several millimeters, it has advantage to be adapted to various pump design. Here, two kinds of micro pumps with the check-valves were fabricated. One is diaphragm pump actuated by vibration of the diaphragm, and another is tube-shaped pump actuated by resonation. The flow quantities of the pumps were evaluated and the results show that both of the pumps have high pumping performance.

STS-93 / Columbia Flight Crew Photo Op & QA at Pad for TCDT

NASA Technical Reports Server (NTRS)

1999-01-01

The primary objective of the STS-93 mission was to deploy the Advanced X-ray Astrophysical Facility, which had been renamed the Chandra X-ray Observatory in honor of the late Indian-American Nobel Laureate Subrahmanyan Chandrasekhar. The mission was launched at 12:31 on July 23, 1999 onboard the space shuttle Columbia. The mission was led by Commander Eileen Collins. The crew was Pilot Jeff Ashby and Mission Specialists Cady Coleman, Steve Hawley and Michel Tognini from the Centre National d'Etudes Spatiales (CNES). This videotape shows a pre-flight press conference. Prior to the astronauts' arrival at the bunker area in front of the launch pad, the narrator discusses some of the training that the astronauts are scheduled to have prior to the launch, particularly the emergency egress procedures. Commander Collins introduces the crew and fields questions from the assembled press. Many questions are asked about the experiences of Commander Collins, and Mission Specialist Coleman as women in NASA. The press conference takes place outside in front of the Shuttle Columbia on the launch pad.

Similar Ratios of Introns to Intergenic Sequence across Animal Genomes.

PubMed

Francis, Warren R; Wörheide, Gert

2017-06-01

One central goal of genome biology is to understand how the usage of the genome differs between organisms. Our knowledge of genome composition, needed for downstream inferences, is critically dependent on gene annotations, yet problems associated with gene annotation and assembly errors are usually ignored in comparative genomics. Here, we analyze the genomes of 68 species across 12 animal phyla and some single-cell eukaryotes for general trends in genome composition and transcription, taking into account problems of gene annotation. We show that, regardless of genome size, the ratio of introns to intergenic sequence is comparable across essentially all animals, with nearly all deviations dominated by increased intergenic sequence. Genomes of model organisms have ratios much closer to 1:1, suggesting that the majority of published genomes of nonmodel organisms are underannotated and consequently omit substantial numbers of genes, with likely negative impact on evolutionary interpretations. Finally, our results also indicate that most animals transcribe half or more of their genomes arguing against differences in genome usage between animal groups, and also suggesting that the transcribed portion is more dependent on genome size than previously thought. © The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

Containerless processing of glass forming melts: D-1, MEA/A-2 experiment 81F01 conducted on STS-61A flight, October 1985

NASA Technical Reports Server (NTRS)

Day, D. E.; Ray, C. S.

1986-01-01

Results of experiment 81F01, which was conducted in the Material Experiment Assembly MEA/A-2 on the D-1 Spacelab Mission (STS-61A), are presented. The general plan of the experiment was to heat, melt, and quench six spherical samples of different glass forming compositions while they were levitated in a single axis acoustic levitator furnace (SAAL). In addition, two non-melting sintered alumina samples were used to check the operational characteristics of the SAAL under reduced gravity conditions. Three of the eight samples were levitated between 1250 and 1500 C before the lack of coolant created an over-temperature condition that caused the SAAL to shut down prematurely. Two of the three samples processed were calcia-gallia-silica and soda-lime-silica glass forming compositions. Evidence of a two to three times increase in the tendency for glass formation was obtained for the calcia-gallia-silica. The final glass appeared reasonably homogeneous even though it was made from hot pressed powders containing deliberate heterogeneities. A photographic record was obtained of the microgravity sample processing sequences.

A Piezoelectric Micromotor with a Stator of φ=1.6 mm and l=4 mm Using Bulk PZT

NASA Astrophysics Data System (ADS)

Cagatay, Serra; Koc, Burhanettin; Moses, Paul; Uchino, Kenji

2004-04-01

The smallest discrete piezoelectric ultrasonic motor using bulk ceramics was developed. We are proposing basically a two-part motor: stator and rotor. The stator of the present motor consists of a hollow metal brass tube with outer diameter of 1.6 mm, inner diameter of 0.8 mm and length of only 4 mm with 2 PZT plates bonded onto it. Owing to the asymmetrical stator surface, two degenerated orthogonal bending modes were slightly split, resulting in a wobbling motion. Thus, the motor can be driven by a single driving source. The rotor is a spring, which is basically different from previous designs, pressed at both ends to the stator by a pair of ferrules. Consequently, the length of the whole motor assembly was reduced significantly; a final motor length of only 5 mm was obtained. The working frequency under zero load was approximately 227-233 kHz. Although the size is small, relatively high power was obtained under an optimized load condition: torque of 0.06 mNm, maximum power of 3.2 mW with a speed of 118 rad/s, and maximum efficiency of 11% under 48 Vrms at 221 kHz.

The genome of the Antarctic-endemic copepod, Tigriopus kingsejongensis.

PubMed

Kang, Seunghyun; Ahn, Do-Hwan; Lee, Jun Hyuck; Lee, Sung Gu; Shin, Seung Chul; Lee, Jungeun; Min, Gi-Sik; Lee, Hyoungseok; Kim, Hyun-Woo; Kim, Sanghee; Park, Hyun

2017-01-01

The Antarctic intertidal zone is continuously subjected to extremely fluctuating biotic and abiotic stressors. The West Antarctic Peninsula is the most rapidly warming region on Earth. Organisms living in Antarctic intertidal pools are therefore interesting for research into evolutionary adaptation to extreme environments and the effects of climate change. We report the whole genome sequence of the Antarctic-endemic harpacticoid copepod Tigriopus kingsejongensi . The 37 Gb raw DNA sequence was generated using the Illumina Miseq platform. Libraries were prepared with 65-fold coverage and a total length of 295 Mb. The final assembly consists of 48 368 contigs with an N50 contig length of 17.5 kb, and 27 823 scaffolds with an N50 contig length of 159.2 kb. A total of 12 772 coding genes were inferred using the MAKER annotation pipeline. Comparative genome analysis revealed that T. kingsejongensis -specific genes are enriched in transport and metabolism processes. Furthermore, rapidly evolving genes related to energy metabolism showed positive selection signatures. The T. kingsejongensis genome provides an interesting example of an evolutionary strategy for Antarctic cold adaptation, and offers new genetic insights into Antarctic intertidal biota. © The Author 2017. Published by Oxford University Press.

KSC-2011-4892

NASA Image and Video Library

2011-07-20

CAPE CANAVERAL, Fla. -- Employees at NASA's Kennedy Space Center in Florida gathered in the Press Site parking lot, with the towering Vehicle Assembly Building in the background on June 27, 2011, to record a wake-up call to Atlantis' STS-135 crew members on NASA’s last voyage aboard a space shuttle. Use of music to awaken astronauts on space missions dates back at least to the Apollo Program, when astronauts returning from the moon were serenaded by their colleagues in mission control with lyrics from popular songs that seemed appropriate for the occasion. Usually picked by flight controllers or by crew members’ friends and family members, most wake-up calls are musical, but sometimes include dialog from movies or TV shows. The recording is usually followed by a call from the CAPCOM in Mission Control, wishing the crew a good morning but because this is the final mission for the Space Shuttle Program many of the NASA centers were given the opportunity to choose a phrase that most represents their best wishes and gratitude for a successful mission. The common element of all these selections is that they promote a sense of camaraderie and esprit de corps among the astronauts and ground support personnel. Photo credit: Cory Huston

KSC-2010-4884

NASA Image and Video Library

2010-09-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, Canadian Space Agency astronaut Chris Hadfield (left) and NASA astronaut Gregory C. Johnson attend a ceremony being held to commemorate the move from Kennedy's Assembly Refurbishment Facility (ARF) to the Vehicle Assembly Building (VAB) of the Space Shuttle Program's final solid rocket booster structural assembly -- the right-hand forward. The move was postponed because of inclement weather. Photo credit: NASA/Kim Shiflett

KSC-2010-4889

NASA Image and Video Library

2010-09-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, United Space Alliance employees gather and hold up a banner at a ceremony being held to commemorate the move from Kennedy's Assembly Refurbishment Facility (ARF) to the Vehicle Assembly Building (VAB) of the Space Shuttle Program's final solid rocket booster structural assembly -- the right-hand forward. The move was postponed because of inclement weather. Photo credit: NASA/Kim Shiflett

Berthing mechanism final test report and program assessment

NASA Technical Reports Server (NTRS)

1988-01-01

The purpose is to document the testing performed on both hardware and software developed under the Space Station Berthing Mechanisms Program. Testing of the mechanism occurred at three locations. Several system components, e.g., actuators and computer systems, were functionally tested before assembly. A series of post assembly tests were performed. The post assembly tests, as well as the dynamic testing of the mechanism, are presented.

Superelastic Ball Bearings: Materials and Design to Avoid Mounting and Dismounting Brinell Damage in an Inaccessible Press-Fit Application-. I ; Design Approach

NASA Technical Reports Server (NTRS)

Dellacorte, Christopher; Howard, S. Adam

2015-01-01

Ball bearings require proper fit and installation into machinery structures (onto shafts and into bearing housings) to ensure optimal performance. For some applications, both the inner and outer race must be mounted with an interference fit and care must be taken during assembly and disassembly to avoid placing heavy static loads between the balls and races otherwise Brinell dent type damage can occur. In this paper, a highly dent resistant superelastic alloy, 60NiTi, is considered for rolling element bearing applications that encounter excessive static axial loading during assembly or disassembly. A small (R8) ball bearing is designed for an application in which access to the bearing races to apply disassembly tools is precluded. First Principles analyses show that by careful selection of materials, raceway curvature and land geometry, a bearing can be designed that allows blind assembly and disassembly without incurring raceway damage due to ball denting. Though such blind assembly applications are uncommon, the availability of bearings with unusually high static load capability may enable more such applications with additional benefits, especially for miniature bearings.

El Nino Election. Grade 12 Lesson. Schools of California Online Resources for Education (SCORE): Connecting California's Classrooms to the World.

ERIC Educational Resources Information Center

Mac Donald, David R.; Karayan, Michael S.

In this lesson plan, the city of Malibu (CA) is at the mercy of the weather phenomenon El Nino. The press secretary for the Mayor of Malibu recognizes an opportunity to turn this potential natural disaster into a re-election campaign. A task force will be assembled to protect the lives and property of the community. Students role play members of…

Higher-order assemblies of BAR domain proteins for shaping membranes.

PubMed

Suetsugu, Shiro

2016-06-01

Most cellular organelles contain lipid bilayer membranes. The earliest characterization of cellular organelles was performed by electron microscopy observation of such membranes. However, the precise mechanisms for shaping the membrane in particular subcellular organelles is poorly understood. Classically, the overall cellular shape, i.e. the shape of the plasma membrane, was thought to be governed by the reorganization of cytoskeletal components such as actin and microtubules. The plasma membrane contains various submicron structures such as clathrin-coated pits, caveolae, filopodia and lamellipodia. These subcellular structures are either invaginations or protrusions and are associated with the cytoskeleton. Therefore, it could be hypothesized that there are membrane-binding proteins that cooperates with cytoskeleton in shaping of plasma membrane organelles. Proteins with the Bin-Amphiphysin-Rvs (BAR) domain connect a variety of membrane shapes to actin filaments. The BAR domains themselves bend the membranes by their rigidity and then mold the membranes into tubules through their assembly as spiral polymers, which are thought to be involved in the various submicron structures. Membrane tubulation by polymeric assembly of the BAR domains is supposed to be regulated by binding proteins, binding lipids and the mechanical properties of the membrane. This review gives an overview of BAR protein assembly, describes the significance of the assembly and discusses how to study the assembly in the context of membrane and cellular morphology. The technical problems encountered in microscopic observation of BAR domain assembly are also discussed. © The Author 2016. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Method of bonding

DOEpatents

Saller, deceased, Henry A.; Hodge, Edwin S.; Paprocki, Stanley J.; Dayton, Russell W.

1987-12-01

1. A method of making a fuel-containing structure for nuclear reactors, comprising providing an assembly comprising a plurality of fuel units; each fuel unit consisting of a core plate containing thermal-neutron-fissionable material, sheets of cladding metal on its bottom and top surfaces, said cladding sheets being of greater width and length than said core plates whereby recesses are formed at the ends and sides of said core plate, and end pieces and first side pieces of cladding metal of the same thickness as the core plate positioned in said recesses, the assembly further comprising a plurality of second side pieces of cladding metal engaging the cladding sheets so as to space the fuel units from one another, and a plurality of filler plates of an acid-dissolvable nonresilient material whose melting point is above 2000.degree. F., each filler plate being arranged between a pair of said second side pieces and the cladding plates of two adjacent fuel units, the filler plates having the same thickness as the second side pieces; the method further comprising enclosing the entire assembly in an envelope; evacuating the interior of the entire assembly through said envelope; applying inert gas under a pressure of about 10,000 psi to the outside of said envelope while at the same time heating the assembly to a temperature above the flow point of the cladding metal but below the melting point of any material of the assembly, whereby the envelope is pressed against the assembly and integral bonds are formed between plates, sheets, first side pieces, and end pieces and between the sheets and the second side pieces; slowly cooling the assembly to room temperature; removing the envelope; and dissolving the filler plates without attacking the cladding metal.

Expedition 38 Press Conference

NASA Image and Video Library

2013-11-06

Expedition 38 backup crew member Reid Wiseman of NASA is seen in quarantine, behind glass, during the final press conference held a day ahead of the launch of Expedition 38 prime crew members; Flight Engineer Koichi Wakata of the Japan Aerospace Exploration Agency, Soyuz Commander Mikhail Tyurin of Roscosmos, and, Flight Engineer Rick Mastracchio of NASA, to the International Space Station, Wednesday, Nov. 6, 2013 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Bill Ingalls)

Engine with exhaust gas recirculation system and variable geometry turbocharger

DOEpatents

Keating, Edward J.

2015-11-03

An engine assembly includes an intake assembly, an internal combustion engine defining a plurality of cylinders and configured to combust a fuel and produce exhaust gas, and an exhaust assembly in fluid communication with a first subset of the plurality of cylinders. Each of the plurality of cylinders are provided in fluid communication with the intake assembly. The exhaust assembly is provided in fluid communication with a first subset of the plurality of cylinders, and a dedicated exhaust gas recirculation system in fluid communication with both a second subset of the plurality of cylinders and with the intake assembly. The dedicated exhaust gas recirculation system is configured to route all of the exhaust gas from the second subset of the plurality of cylinders to the intake assembly. Finally, the engine assembly includes a turbocharger having a variable geometry turbine in fluid communication with the exhaust assembly.

An assembly process model based on object-oriented hierarchical time Petri Nets

NASA Astrophysics Data System (ADS)

Wang, Jiapeng; Liu, Shaoli; Liu, Jianhua; Du, Zenghui

2017-04-01

In order to improve the versatility, accuracy and integrity of the assembly process model of complex products, an assembly process model based on object-oriented hierarchical time Petri Nets is presented. A complete assembly process information model including assembly resources, assembly inspection, time, structure and flexible parts is established, and this model describes the static and dynamic data involved in the assembly process. Through the analysis of three-dimensional assembly process information, the assembly information is hierarchically divided from the whole, the local to the details and the subnet model of different levels of object-oriented Petri Nets is established. The communication problem between Petri subnets is solved by using message database, and it reduces the complexity of system modeling effectively. Finally, the modeling process is presented, and a five layer Petri Nets model is established based on the hoisting process of the engine compartment of a wheeled armored vehicle.

Cerium migration during PEM fuel cell assembly and operation

DOE PAGES

Baker, Andrew M.; Torraco, Dennis; Judge, Elizabeth J.; ...

2015-09-14

Cerium migration between PEM fuel cell components is influenced by potential-driven mobility, ionic diffusion, and gradients in water content. These factors were investigated in ex situ experiments and in operating fuel cells. Potential-induced migration was measured ex situ in hydrated window cells. Cerium-containing MEAs were also fabricated and tested under ASTs. MEA disassembly and subsequent XRF analysis were used to observe rapid cerium migration during cell assembly and operation. During MEA hot pressing, humidification, and low RH operation at OCV, ionic diffusion causes uniform migration from the membrane into the catalyst layers. During high RH operation at OCV, in-plane ceriummore » gradients arise due to variations in water content. These gradients may diminish the scavenging efficacy of cerium by reducing its proximity to generated radicals.« less

Elastic airtight container for the compaction of air-sensitive materials

NASA Astrophysics Data System (ADS)

Shoulders, W. Taylor; Locke, Richard; Gaume, Romain M.

2016-06-01

We report on the design and fabrication of a simple and versatile elastic canister for the compaction and hot-pressing of air-sensitive materials. This device consists of a heated double-ended floating die assembly, enclosed in a compressible stainless steel bellows that allows the action of an external hydraulic press in a uniaxial motion. The enclosure is fitted with vacuum, gas, and electrical feedthroughs to allow for atmosphere control, heating, and in situ process monitoring. The overall chamber is compact enough to be portable and transferrable into and out of a standard laboratory glovebox, thus eliminating the problem of exposing samples to ambient atmosphere during loading and unloading. Our design has been tested up to 600 °C and 7500 kg-force applied load, conditions within which transparent ceramics of anhydrous halides can be produced.

KSC-2010-4883

NASA Image and Video Library

2010-09-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, John Casper, Assistant Space Shuttle Program manager and Kennedy Center Director Bob Cabana talk with each other during a ceremony being held to commemorate the move from Kennedy's Assembly Refurbishment Facility (ARF) to the Vehicle Assembly Building (VAB) of the Space Shuttle Program's final solid rocket booster structural assembly -- the right-hand forward. The move was postponed because of inclement weather. Photo credit: NASA/Kim Shiflett

MEGAnnotator: a user-friendly pipeline for microbial genomes assembly and annotation.

PubMed

Lugli, Gabriele Andrea; Milani, Christian; Mancabelli, Leonardo; van Sinderen, Douwe; Ventura, Marco

2016-04-01

Genome annotation is one of the key actions that must be undertaken in order to decipher the genetic blueprint of organisms. Thus, a correct and reliable annotation is essential in rendering genomic data valuable. Here, we describe a bioinformatics pipeline based on freely available software programs coordinated by a multithreaded script named MEGAnnotator (Multithreaded Enhanced prokaryotic Genome Annotator). This pipeline allows the generation of multiple annotated formats fulfilling the NCBI guidelines for assembled microbial genome submission, based on DNA shotgun sequencing reads, and minimizes manual intervention, while also reducing waiting times between software program executions and improving final quality of both assembly and annotation outputs. MEGAnnotator provides an efficient way to pre-arrange the assembly and annotation work required to process NGS genome sequence data. The script improves the final quality of microbial genome annotation by reducing ambiguous annotations. Moreover, the MEGAnnotator platform allows the user to perform a partial annotation of pre-assembled genomes and includes an option to accomplish metagenomic data set assemblies. MEGAnnotator platform will be useful for microbiologists interested in genome analyses of bacteria as well as those investigating the complexity of microbial communities that do not possess the necessary skills to prepare their own bioinformatics pipeline. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Simulation of the Press Hardening Process and Prediction of the Final Mechanical Material Properties

NASA Astrophysics Data System (ADS)

Hochholdinger, Bernd; Hora, Pavel; Grass, Hannes; Lipp, Arnulf

2011-08-01

Press hardening is a well-established production process in the automotive industry today. The actual trend of this process technology points towards the manufacturing of parts with tailored properties. Since the knowledge of the mechanical properties of a structural part after forming and quenching is essential for the evaluation of for example the crash performance, an accurate as possible virtual assessment of the production process is more than ever necessary. In order to achieve this, the definition of reliable input parameters and boundary conditions for the thermo-mechanically coupled simulation of the process steps is required. One of the most important input parameters, especially regarding the final properties of the quenched material, is the contact heat transfer coefficient (IHTC). The CHTC depends on the effective pressure or the gap distance between part and tool. The CHTC at different contact pressures and gap distances is determined through inverse parameter identification. Furthermore a simulation strategy for the subsequent steps of the press hardening process as well as adequate modeling approaches for part and tools are discussed. For the prediction of the yield curves of the material after press hardening a phenomenological model is presented. This model requires the knowledge of the microstructure within the part. By post processing the nodal temperature history with a CCT diagram the quantitative distribution of the phase fractions martensite, bainite, ferrite and pearlite after press hardening is determined. The model itself is based on a Hockett-Sherby approach with the Hockett-Sherby parameters being defined in function of the phase fractions and a characteristic cooling rate.

Anaerobic digestion of pressed off leachate from the organic fraction of municipal solid waste

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nayono, Satoto E.; Institute of Biology for Engineers and Biotechnology of Wastewater, University of Karlsruhe, Am Fasanengarten, 76131 Karlsruhe; Winter, Josef, E-mail: josef.winter@iba.uka.d

2010-10-15

A highly polluted liquid ('press water') was obtained from the pressing facility for the organic fraction of municipal solid waste in a composting plant. Methane productivity of the squeezed-off leachate was investigated in batch assays. To assess the technical feasibility of 'press water' as a substrate for anaerobic digestion, a laboratory-scale glass column reactor was operated semi-continuously at 37 {sup o}C. A high methane productivity of 270 m{sup -3} CH{sub 4} ton{sup -1} COD{sub added} or 490 m{sup -3} CH{sub 4} ton{sup -1} VS{sub added} was achieved in the batch experiment. The semi-continuously run laboratory-scale reactor was initially operated atmore » an organic loading rate of 10.7 kg COD m{sup -3} d{sup -1}. The loading was increased to finally 27.7 kg COD m{sup -3} d{sup -1}, corresponding to a reduction of the hydraulic retention time from initially 20 to finally 7.7 days. During the digestion, a stable elimination of organic material (measured as COD elimination) of approximately 60% was achieved. Linearly with the increment of the OLR, the volumetric methane production of the reactor increased from 2.6 m{sup 3} m{sub reactor}{sup -3} d{sup -1} to 7.1 m{sup 3} m{sub reactor}{sup -3} d{sup -1}. The results indicated that 'press water' from the organic fraction of municipal solid waste was a suitable substrate for anaerobic digestion which gave a high biogas yield even at very high loading rates.« less

De novo transcriptome assembly of a fern, Lygodium japonicum, and a web resource database, Ljtrans DB.

PubMed

Aya, Koichiro; Kobayashi, Masaaki; Tanaka, Junmu; Ohyanagi, Hajime; Suzuki, Takayuki; Yano, Kenji; Takano, Tomoyuki; Yano, Kentaro; Matsuoka, Makoto

2015-01-01

During plant evolution, ferns originally evolved as a major vascular plant with a distinctive life cycle in which the haploid and diploid generations are completely separated. However, the low level of genetic resources has limited studies of their physiological events, as well as hindering research on the evolutionary history of land plants. In this study, to identify a comprehensive catalog of transcripts and characterize their expression traits in the fern Lygodium japonicum, nine different RNA samples isolated from prothalli, trophophylls, rhizomes and sporophylls were sequenced using Roche 454 GS-FLX and Illumina HiSeq sequencers. The hybrid assembly of the high-quality 454 GS-FLX and Illumina HiSeq reads generated a set of 37,830 isoforms with an average length of 1,444 bp. Using four open reading frame (ORF) predictors, 38,142 representative ORFs were identified from a total of 37,830 transcript isoforms and 95 contigs, which were annotated by searching against several public databases. Furthermore, an orthoMCL analysis using the protein sequences of L. japonicum and five model plants revealed various sets of lineage-specific genes, including those detected among land plant lineages and those detected in only L. japonicum. We have also examined the expression patterns of all contigs/isoforms, along with the life cycle of L. japonicum, and identified the tissue-specific transcripts using statistical expression analyses. Finally, we developed a public web resource, the L. japonicum transcriptome database at http://bioinf.mind.meiji.ac.jp/kanikusa/, which provides important opportunities to accelerate molecular research in ferns. © The Author 2014. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Deep Sequencing of the Fruit Transcriptome and Lipid Accumulation in a Non-Seed Tissue of Chinese Tallow, a Potential Biofuel Crop.

PubMed

Divi, Uday K; Zhou, Xue-Rong; Wang, Penghao; Butlin, Jamie; Zhang, Dong-Mei; Liu, Qing; Vanhercke, Thomas; Petrie, James R; Talbot, Mark; White, Rosemary G; Taylor, Jennifer M; Larkin, Philip; Singh, Surinder P

2016-01-01

Chinese tallow (Triadica sebifera) is a valuable oilseed-producing tree that can grow in a variety of conditions without competing for food production, and is a promising biofuel feedstock candidate. The fruits are unique in that they contain both saturated and unsaturated fat present in the tallow and seed layer, respectively. The tallow layer is poorly studied and is considered only as an external fatty deposition secreted from the seed. In this study we show that tallow is in fact a non-seed cellular tissue capable of triglyceride synthesis. Knowledge of lipid synthesis and storage mechanisms in tissues other than seed is limited but essential to generate oil-rich biomass crops. Here, we describe the annotated transcriptome assembly generated from the fruit coat, tallow and seed tissues of Chinese tallow. The final assembly was functionally annotated, allowing for the identification of candidate genes and reconstruction of lipid pathways. A tallow tissue-specific paralog for the transcription factor gene WRINKLED1 (WRI1) and lipid droplet-associated protein genes, distinct from those expressed in seed tissue, were found to be active in tallow, underpinning the mode of oil synthesis and packaging in this tissue. Our data have established an excellent knowledge base that can provide genetic and biochemical insights for engineering non-seed tissues to accumulate large amounts of oil. In addition to the large data set of annotated transcripts, the study also provides gene-based simple sequence repeat and single nucleotide polymorphism markers. © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Long-read sequencing of nascent RNA reveals coupling among RNA processing events.

PubMed

Herzel, Lydia; Straube, Korinna; Neugebauer, Karla M

2018-06-14

Pre-mRNA splicing is accomplished by the spliceosome, a megadalton complex that assembles de novo on each intron. Because spliceosome assembly and catalysis occur cotranscriptionally, we hypothesized that introns are removed in the order of their transcription in genomes dominated by constitutive splicing. Remarkably little is known about splicing order and the regulatory potential of nascent transcript remodeling by splicing, due to the limitations of existing methods that focus on analysis of mature splicing products (mRNAs) rather than substrates and intermediates. Here, we overcome this obstacle through long-read RNA sequencing of nascent, multi-intron transcripts in the fission yeast Schizosaccharomyces pombe Most multi-intron transcripts were fully spliced, consistent with rapid cotranscriptional splicing. However, an unexpectedly high proportion of transcripts were either fully spliced or fully unspliced, suggesting that splicing of any given intron is dependent on the splicing status of other introns in the transcript. Supporting this, mild inhibition of splicing by a temperature-sensitive mutation in prp2 , the homolog of vertebrate U2AF65, increased the frequency of fully unspliced transcripts. Importantly, fully unspliced transcripts displayed transcriptional read-through at the polyA site and were degraded cotranscriptionally by the nuclear exosome. Finally, we show that cellular mRNA levels were reduced in genes with a high number of unspliced nascent transcripts during caffeine treatment, showing regulatory significance of cotranscriptional splicing. Therefore, overall splicing of individual nascent transcripts, 3' end formation, and mRNA half-life depend on the splicing status of neighboring introns, suggesting crosstalk among spliceosomes and the polyA cleavage machinery during transcription elongation. © 2018 Herzel et al.; Published by Cold Spring Harbor Laboratory Press.

Prove Out of Automated Assembly Line for M564 Delay Arming Mechanisms by Acquisition of 40,000 Units Assembled by Automated Line.

DTIC Science & Technology

1981-01-22

Arsenal Contract DUAA25-69-C- 0301 dated 3-10-69 for "Services to design and develop an automated line for assembly of Safety Adapter, Fuze, 1564...determine the line will function at the design rate of 5,000 acceptable assemblies per day and collect production data for incorporation into final...0,000 M564 Delay Arming Mechanisms of the line: Determine that the line will function at the design rate of 5,000. acceotabie asswml I es/day. Collect

Development of cable drive systems for an automated assembly project

NASA Technical Reports Server (NTRS)

Monroe, Charles A., Jr.

1990-01-01

In a robotic assembly project, a method was needed to accurately position a robot and a structure which the robot was to assemble. The requirements for high precision and relatively long travel distances dictated the use of cable drive systems. The design of the mechanisms used in translating the robot and in rotating the assembly under construction is discussed. The design criteria are discussed, and the effect of particular requirements on the design is noted. Finally, the measured performance of the completed mechanism is compared with design requirements.

Payload spin assembly for the commercial Titan launch vehicle

NASA Technical Reports Server (NTRS)

Robinson, Wilf; Pech, Greg

1991-01-01

A contract was completed to design, build, and test a Payload Spin Assembly (PSA) for installation onto the Martin Marietta Titan 3 Commercial launch vehicle. This assembly provides launch support for satellite payloads up to 5783 kilograms (6.37 tons) and controls release, spin-up, and final separation of the satellite from the second stage. Once separated, the satellite's Perigee Kick Motor (PKM) boosts the satellite into its transfer orbit. The first successful flight occurred December 31, 1989. Requirements, design, test, and problems associated with this mechanical assembly are discussed.

The versatility of a truss mounted mobile transporter for in-space construction

NASA Technical Reports Server (NTRS)

Bush, Harold G.; Lake, Mark S.; Watson, Judith J.; Heard, Walter L., Jr.

1988-01-01

The Mobile Transporter (MT) evolution from early erectable structures assembly activities is detailed. The MT operational features which are required to support astronauts performing on-orbit structure construction or spacecraft assembly functions are presented and discussed. Use of the MT to perform a variety of assembly functions is presented. Estimated EVA assembly times for a precision segmented reflector approximately 20 m in diameter are presented. The EVA/MT technique under study for construction of the reflector (and the entire spacecraft) is illustrated. Finally, the current status of development activities and test results involving the MT and Space Station structural assembly are presented.

Spherical primary optical telescope (SPOT) segments

NASA Astrophysics Data System (ADS)

Hall, Christopher; Hagopian, John; DeMarco, Michael

2012-09-01

The spherical primary optical telescope (SPOT) project is an internal research and development program at NASA Goddard Space Flight Center. The goals of the program are to develop a robust and cost effective way to manufacture spherical mirror segments and demonstrate a new wavefront sensing approach for continuous phasing across the segmented primary. This paper focuses on the fabrication of the mirror segments. Significant cost savings were achieved through the design, since it allowed the mirror segments to be cast rather than machined from a glass blank. Casting was followed by conventional figuring at Goddard Space Flight Center. After polishing, the mirror segments were mounted to their composite assemblies. QED Technologies used magnetorheological finishing (MRF®) for the final figuring. The MRF process polished the mirrors while they were mounted to their composite assemblies. Each assembly included several magnetic invar plugs that extended to within an inch of the face of the mirror. As part of this project, the interaction between the MRF magnetic field and invar plugs was evaluated. By properly selecting the polishing conditions, MRF was able to significantly improve the figure of the mounted segments. The final MRF figuring demonstrates that mirrors, in the mounted configuration, can be polished and tested to specification. There are significant process capability advantes due to polishing and testing the optics in their final, end-use assembled state.

Characteristics of a Power Line Used as a VLF Antenna.

DTIC Science & Technology

1982-05-01

were glass melamine . Assemblies of 12 layers were fabricated at El Segundo, CA and shipped by air to Tromso, Norway for final assembly of the full 120...sciences, applied electronics, semiconductor crystal and device physics, radiometric Imaging; millimeter-wave and microwave technology. Information

Fabrication Control Plan for ORNL RH-LOCA ATF Test Specimens to be Irradiated in the ATR

DOE Office of Scientific and Technical Information (OSTI.GOV)

Field, Kevin G.; Howard, Richard; Teague, Michael

2014-06-01

The purpose of this fabrication plan is (1) to summarize the design of a set of rodlets that will be fabricated and then irradiated in the Advanced Test Reactor (ATR) and (2) provide requirements for fabrication and acceptance criteria for inspections of the Light Water Reactor (LWR) – Accident Tolerant Fuels (ATF) rodlet components. The functional and operational (F&OR) requirements for the ATF program are identified in the ATF Test Plan. The scope of this document only covers fabrication and inspections of rodlet components detailed in drawings 604496 and 604497. It does not cover the assembly of these items tomore » form a completed test irradiation assembly or the inspection of the final assembly, which will be included in a separate INL final test assembly specification/inspection document. The controls support the requirements that the test irradiations must be performed safely and that subsequent examinations must provide valid results.« less

Expanded Analysis of Hot Isostatic Pressed Iodine-Loaded Silver-Exchanged Mordenite

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jubin, R. T.; Bruffey, S. H.; Patton, K. K.

2014-09-30

Reduced silver-exchanged mordenite (Ag0Z) is being evaluated as a potential material to control the release of radioactive iodine that is released during the reprocessing of used nuclear fuel into the plant off-gas streams. The purpose of this study was to determine if hot pressing could directly convert this iodine loaded sorbent into a waste form suitable for long-term disposition. The minimal pretreatment required for production of pressed pellets makes hot pressing a technically and economically desirable process. Initial scoping studies utilized hot uniaxial pressing (HUPing) to prepare samples of non-iodine-loaded reduced silver exchanged mordenite (Ag0Z). The resulting samples were verymore » fragile due to the low pressure (~ 28 MPa) used. It was recommended that hot isostatic pressing (HIPing), performed at higher temperatures and pressures, be investigated. HIPing was carried out in two phases, with a third and final phase currently underway. Phase I evaluated the effects of pressure and temperature conditions on the manufacture of a pressed sample. The base material was an engineered form of silver zeolite. Six samples of Ag0Z and two samples of I-Ag0Z were pressed. It was found that HIPing produced a pressed pellet of high density. Analysis of each pressed pellet by scanning electron microscopy-energy dispersive spectrophotometry (SEM-EDS) and X-ray diffraction (XRD) demonstrated that under the conditions used for pressing, the majority of the material transforms into an amorphous structure. The only crystalline phase observed in the pressed Ag0Z material was SiO2. For the samples loaded with iodine (I-Ag0Z) iodine was present as AgI clusters at low temperatures, and transformed into AgIO4 at high temperatures. Surface mapping and EDS demonstrate segregation between silver iodide phases and silicon dioxide phases. Based on the results of the Phase I study, an expanded test matrix was developed to examine the effects of multiple source materials, compositional variations, and an expanded temperature range. Each sample was analyzed with the approach used in Phase I. In all cases, there is nothing in the SEM or XRD analyses that indicates creation of any AgI-containing silicon phase, with the samples being found to be largely amorphous. Phase III of this study has been initiated and is the final phase of scoping tests. It will expand upon the test matrix completed in Phase II and will examine the durability of the pressed pellets through product consistency testing (PCT) studies. Transformation of the component material into a well-characterized iodine-containing mineral phase would be desirable. This would limit the additional experimental testing and modeling required to determine the long-term stability of the pressed pellet, as much of that information has already been learned for several common iodine-containing minerals. However, this is not an absolute requirement, especially if pellets produced by hot isostatic pressing can be demonstrated through initial PCT studies to retain iodine well despite their amorphous composition.« less

Improving amphibian genomic resources: a multitissue reference transcriptome of an iconic invader.

PubMed

Richardson, Mark F; Sequeira, Fernando; Selechnik, Daniel; Carneiro, Miguel; Vallinoto, Marcelo; Reid, Jack G; West, Andrea J; Crossland, Michael R; Shine, Richard; Rollins, Lee A

2018-01-01

Cane toads (Rhinella marina) are an iconic invasive species introduced to 4 continents and well utilized for studies of rapid evolution in introduced environments. Despite the long introduction history of this species, its profound ecological impacts, and its utility for demonstrating evolutionary principles, genetic information is sparse. Here we produce a de novo transcriptome spanning multiple tissues and life stages to enable investigation of the genetic basis of previously identified rapid phenotypic change over the introduced range. Using approximately 1.9 billion reads from developing tadpoles and 6 adult tissue-specific cDNA libraries, as well as a transcriptome assembly pipeline encompassing 100 separate de novo assemblies, we constructed 62 202 transcripts, of which we functionally annotated ∼50%. Our transcriptome assembly exhibits 90% full-length completeness of the Benchmarking Universal Single-Copy Orthologs data set. Robust assembly metrics and comparisons with several available anuran transcriptomes and genomes indicate that our cane toad assembly is one of the most complete anuran genomic resources available. This comprehensive anuran transcriptome will provide a valuable resource for investigation of genes under selection during invasion in cane toads, but will also greatly expand our general knowledge of anuran genomes, which are underrepresented in the literature. The data set is publically available in NCBI and GigaDB to serve as a resource for other researchers. © The Authors 2017. Published by Oxford University Press.

Influence of hot isostatic pressing on ZrO2-CaO dental ceramics properties.

PubMed

Gionea, Alin; Andronescu, Ecaterina; Voicu, Georgeta; Bleotu, Coralia; Surdu, Vasile-Adrian

2016-08-30

Different hot isostatic pressing conditions were used to obtain zirconia ceramics, in order to assess the influence of HIP on phase transformation, compressive strength, Young's modulus and density. First, CaO stabilized zirconia powder was synthesized through sol-gel method, using zirconium propoxide, calcium isopropoxide and 2-metoxiethanol as precursors, then HIP treatment was applied to obtain final dense ceramics. Ceramics were morphologically and structurally characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Density measurements, compressive strength and Young's modulus tests were also performed in order to evaluate the effect of HIP treatment. The zirconia powders heat treated at 500°C for 2h showed a pure cubic phase with average particle dimension about 70nm. The samples that were hot isostatic pressed presented a mixture of monoclinic-tetragonal or monoclinic-cubic phases, while for pre-sintered samples, cubic zirconia was the single crystalline form. Final dense ceramics were obtained after HIP treatment, with relative density values higher than 94%. ZrO2-CaO ceramics presented high compressive strength, with values in the range of 500-708.9MPa and elastic behavior with Young's modulus between 1739MPa and 4372MPa. Finally zirconia ceramics were tested for biocompatibility allowing the normal development of MG63 cells in vitro. Copyright © 2015 Elsevier B.V. All rights reserved.

KSC-2014-3527

NASA Image and Video Library

2014-08-14

CAPE CANAVERAL, Fla. – A storm is brewing over Launch Complex 39 at NASA’s Kennedy Space Center in Florida. At left are the news networks' facilities on the NASA Press Site. At right is the behemoth Vehicle Assembly Building. Kennedy's Ground Support Development and Operations Program is hard at work transforming the center's facilities into a multi-user spaceport, when the weather permits. For more on Kennedy Space Center, visit http://www.nasa.gov/kennedy. Photo credit: NASA/Ben Smegelsky

Expressing the sense of Congress regarding the immediate and unconditional release of Aung San Suu Kyi, a meaningful tripartite political dialogue toward national reconciliation, and the full restoration of democracy, freedom of assembly, freedom of movement, freedom of speech, freedom of the press, and internationally recognized human rights for all Burmese citizens.

THOMAS, 111th Congress

Rep. King, Peter T. [R-NY-3

2009-11-06

House - 07/26/2010 Referred to the Subcommittee on Immigration, Citizenship, Refugees, Border Security, and International Law. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

Developing a Survey to Assess the Impact of Sociolinguistic Factors on the Morale of Airmen Stationed in Non-English Speaking Countries

DTIC Science & Technology

1989-07-11

evolve to mediate communication between the two groups (1976:136). Based on this hypothesis, Schumann charts the language learning situation of... categorize , collate and summarize the survey data. (6) Critique the questions; pretest and revise. (7) Assemble the questionnaire. (8) Administer the...The Dilemas of U.S. Forces in Germany. Boulder: Vestview Press. Oberg, Kalvero (1960). Cultural Shock: Adjustments to lew Cultural Environments

Macroscopic Crosslinked Neat Carbon Nanotube Materials and CNT/Carbon Fiber Hybrid Composites: Supermolecular Structure and New Failure Mode Study

DTIC Science & Technology

2015-10-01

Materials; CRC Press, 1997. (70) Zhang, Y.; Zheng, L.; Sun , G.; Zhan, Z.; Liao, K. Failure Mechanisms of Carbon Nanotube Fibers under Different...Buehler, M. J. Mesoscale Modeling of Mechanics of Carbon Nanotubes: Self-Assembly, Self-Folding, and Fracture . J. Mater. Res. 2006, 21 (11), 2855–2869...close surface contact between CNTs to substantially improve the load transfer and mechanical properties. We also revealed that extremely low

KENNEDY SPACE CENTER, FLA. -- A pair of breeding ospreys have taken up residence in a nest constructed on a speaker pole in the lower parking lot of the KSC Press Site. Eggs have been sighted in the nest. The NASA logo in the background is painted on an outer wall of the 525-foot-tall Vehicle Assembly Building nearby. Known as a fish hawk, the osprey selects sites of opportunity in which to nest -- from trees and telephone poles to rocks or even flat ground. In North America, it is found from Alaska and Newfoundland to Florida and the Gulf Coast. Osprey nests are found throughout the Kennedy Space Center and surrounding Merritt Island National Wildlife Refuge.

NASA Image and Video Library

2004-01-14

KENNEDY SPACE CENTER, FLA. -- A pair of breeding ospreys have taken up residence in a nest constructed on a speaker pole in the lower parking lot of the KSC Press Site. Eggs have been sighted in the nest. The NASA logo in the background is painted on an outer wall of the 525-foot-tall Vehicle Assembly Building nearby. Known as a fish hawk, the osprey selects sites of opportunity in which to nest -- from trees and telephone poles to rocks or even flat ground. In North America, it is found from Alaska and Newfoundland to Florida and the Gulf Coast. Osprey nests are found throughout the Kennedy Space Center and surrounding Merritt Island National Wildlife Refuge.

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 backup crew member Elena Serova of the Russian Federal Space Agency, Roscosmos, is seen in quarantine, behind glass, during the final press conference held ahead of the launch of Expedition 39 prime crew members; Soyuz Commander Alexander Skvortsov of the Russian Federal Space Agency, Flight Engineer Steve Swanson of NASA, and Flight Engineer Oleg Artemyev of Roscosmos, to the International Space Station, Monday, March 24, 2014 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Bill Ingalls)

Expedition 38 Press Conference

NASA Image and Video Library

2013-11-06

Expedition 38 backup crew member Alexander Gerst of the European Space Agency is seen in quarantine, behind glass, during the final press conference held a day ahead of the launch of Expedition 38 prime crew members; Flight Engineer Koichi Wakata of the Japan Aerospace Exploration Agency, Soyuz Commander Mikhail Tyurin of Roscosmos, and, Flight Engineer Rick Mastracchio of NASA, to the International Space Station, Wednesday, Nov. 6, 2013 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Bill Ingalls)

Expedition 39 Press Conference

NASA Image and Video Library

2014-03-24

Expedition 39 backup crew member Aleksandr Samokutyaev of the Russian Federal Space Agency, Roscosmos, is seen in quarantine, behind glass, during the final press conference held ahead of the launch of Expedition 39 prime crew members; Soyuz Commander Alexander Skvortsov of the Russian Federal Space Agency, Flight Engineer Steve Swanson of NASA, and Flight Engineer Oleg Artemyev of Roscosmos, to the International Space Station, Monday, March 24, 2014 at the Cosmonaut Hotel in Baikonur, Kazakhstan. Photo Credit: (NASA/Bill Ingalls)

The Search for a Cold War Grand Strategy: NSC 68 & 162

DTIC Science & Technology

2014-05-22

Robert Dallek, Harry S. Truman (New York: Times Books, 2008); Ernest R. May, American Cold War Strategy (New York: Bedford Books of St. Martin’s Press...Gave the Soviets the Atomic Bomb (New Haven: Yale University Press, 2009), 119. 32Robert C. Williams , Klaus Fuchs, Atom Spy (Cambridge, MA: Harvard...possibilities, including preemptive buying.”52 Dr. Ernest O. Lawrence was the final consultant engaged by the State-Defense Policy Review Group. The

Sesamin and sesamolin as unexpected contaminants in various cold-pressed plant oils: NP-HPLC/FLD/DAD and RP-UPLC-ESI/MS(n) study.

PubMed

Górnaś, Paweł; Siger, Aleksander; Pugajeva, Iveta; Segliņa, Dalija

2014-04-01

Thirteen cold-pressed oils (Japanese quince seed, black caraway, flaxseed, rapeseed, hemp, peanut, sunflower, pumpkin, hazelnut, poppy, walnut, almond and sesame oil) manufactured by the same company over a 2-year period (2011-12) were assessed for lipophilic compounds. The presence of sesamin and sesamolin, two characteristic lignans of sesame oil, were detected in all tested plant oils. Both lignans were identified by NP-HPLC/FLD/DAD and confirmed by a RP-UPLC-ESI/MS(n) method. The lowest amount of sesamin and sesamolin was found for Japanese quince seed oil (0.10 and 0.27 mg/100 g), and the highest, excluding sesame oil, for almond oil (36.21 and 105.42 mg/100 g, respectively). The highly significant correlation between sesamolin and sesamin concentrations was found in all samples tested (r = 0.9999; p < 0.00001). These results indicate contamination of cold-pressed oils from the same source. This investigation highlights the fact that increasing the range of products manufactured by the same company can contribute to a lesser regard for the quality of the final product. Moreover, less attention paid to the quality of final product can be related to the health risks of consumers especially sensitive to allergens. Therefore, proper cleaning of processing equipment is needed to prevent cross-contact of cold-pressed oils.

Expedition 19 Soyuz Assembly

NASA Image and Video Library

2009-06-09

The Soyuz TMA-14 spacecraft, escape tower, first, second and third stages are seen after final assembly Monday, March 23, 2009 at the Baikonur Cosmodrome in Kazakhstan. The Soyuz is scheduled to launch the crew of Expedition 19 and a spaceflight participant on March 26, 2009. Photo Credit: (NASA/Bill Ingalls)

Self-assembled single-crystal silicon circuits on plastic

PubMed Central

Stauth, Sean A.; Parviz, Babak A.

2006-01-01

We demonstrate the use of self-assembly for the integration of freestanding micrometer-scale components, including single-crystal, silicon field-effect transistors (FETs) and diffusion resistors, onto flexible plastic substrates. Preferential self-assembly of multiple microcomponent types onto a common platform is achieved through complementary shape recognition and aided by capillary, fluidic, and gravitational forces. We outline a microfabrication process that yields single-crystal, silicon FETs in a freestanding, powder-like collection for use with self-assembly. Demonstrations of self-assembled FETs on plastic include logic inverters and measured electron mobility of 592 cm2/V-s. Finally, we extend the self-assembly process to substrates each containing 10,000 binding sites and realize 97% self-assembly yield within 25 min for 100-μm-sized elements. High-yield self-assembly of micrometer-scale functional devices as outlined here provides a powerful approach for production of macroelectronic systems. PMID:16968780

Development of Duplex Stainless Steels by Field-Assisted Hot Pressing: Influence of the Particle Size and Morphology of the Powders on the Final Mechanical Properties

NASA Astrophysics Data System (ADS)

García-Junceda, A.; Rincón, M.; Torralba, J. M.

2018-01-01

The feasibility of processing duplex stainless steels with promising properties using a powder metallurgical route, including the consolidation by field-assisted hot pressing, is assessed in this investigation. The influence of the particle size and morphology of the raw austenitic and ferritic powders on the final microstructure and properties is also evaluated for an austenitic content of 60 wt pct. In addition, the properties of a new microconstituent generated between the initial constituents are analyzed. The maximum sintered density (98.9 pct) and the best mechanical behavior, in terms of elastic modulus, nanohardness, yield strength, ultimate tensile strength, and ductility, are reached by the duplex stainless steel processed with austenitic and ferritic gas atomized stainless steel powders.

Software-assisted stacking of gene modules using GoldenBraid 2.0 DNA-assembly framework.

PubMed

Vazquez-Vilar, Marta; Sarrion-Perdigones, Alejandro; Ziarsolo, Peio; Blanca, Jose; Granell, Antonio; Orzaez, Diego

2015-01-01

GoldenBraid (GB) is a modular DNA assembly technology for plant multigene engineering based on type IIS restriction enzymes. GB speeds up the assembly of transcriptional units from standard genetic parts and facilitates the stacking of several genes within the same T-DNA in few days. GBcloning is software-assisted with a set of online tools. The GBDomesticator tool assists in the adaptation of DNA parts to the GBstandard. The combination of GB-adapted parts to build new transcriptional units is assisted by the GB TU Assembler tool. Finally, the assembly of multigene modules is simulated by the GB Binary Assembler. All the software tools are available at www.gbcloning.org . Here, we describe in detail the assembly methodology to create a multigene construct with three transcriptional units for polyphenol metabolic engineering in plants.

Natural Hazards and the press in the western Mediterranean region

NASA Astrophysics Data System (ADS)

Llasat-Botija, M.; Llasat, M. C.; López, L.

2007-07-01

This study analyses press articles published between 1982 and 2005 in an attempt to describe the social perception of natural hazards in Catalonia. The articles included in the database have been classified according to different types of risk. In addition, the study examines the evolution of each type of risk in the press coverage during the study period. Finally, the results have been compared to data provided by insurance companies with respect to compensations paid out for damages. Conclusions show that floods are the most important natural hazard in the region, but that the number of headlines for each event is greater in the case of snowfalls and forest fires. Factors such as the season of the year, the proximity of the affected region to the capital, the topical issues at the time, and the presence of other important news must be considered when the impact in the press is analysed.

Collisional and Dynamical Evolution of Planetary Systems

NASA Technical Reports Server (NTRS)

Weidenschilling, Stuart J.

2004-01-01

Senior Scientst S. J. Weidenschilling presents his final administrative report in the research program entitled "Collisional and Dynamical Evolution of Planetary Systems," on which he was the Principal Investigator. This research program produced the following publications: 1) "Jumping Jupiters" in binary star systems. F. Marzari, S. J. Weidenschilling, M. Barbieri and V. Granata. Astrophys. J., in press, 2005; 2) Formation of the cores of the outer planets. To appear in "The Outer Planets" (R. Kallenbach, ED), ISSI Conference Proceedings (Space Sci. Rev.), in press, 2005; 3) Accretion dynamics and timescales: Relation to chondrites. S. J. Weidenschilling and J. Cuzzi. In Meteorites and the Early Solar System LI (D. Lauretta et al., Eds.), Univ. of Arizona Press, 2005; 4) Asteroidal heating and thermal stratification of the asteroid belt. A. Ghosh, S. J.Weidenschilling, H. Y. McSween, Jr. and A. Rubin. In Meteorites and the Early Solar System I1 (D. Lauretta et al., Eds.), Univ. of Arizona Press, 2005.

Information-optimal genome assembly via sparse read-overlap graphs.

PubMed

Shomorony, Ilan; Kim, Samuel H; Courtade, Thomas A; Tse, David N C

2016-09-01

In the context of third-generation long-read sequencing technologies, read-overlap-based approaches are expected to play a central role in the assembly step. A fundamental challenge in assembling from a read-overlap graph is that the true sequence corresponds to a Hamiltonian path on the graph, and, under most formulations, the assembly problem becomes NP-hard, restricting practical approaches to heuristics. In this work, we avoid this seemingly fundamental barrier by first setting the computational complexity issue aside, and seeking an algorithm that targets information limits In particular, we consider a basic feasibility question: when does the set of reads contain enough information to allow unambiguous reconstruction of the true sequence? Based on insights from this information feasibility question, we present an algorithm-the Not-So-Greedy algorithm-to construct a sparse read-overlap graph. Unlike most other assembly algorithms, Not-So-Greedy comes with a performance guarantee: whenever information feasibility conditions are satisfied, the algorithm reduces the assembly problem to an Eulerian path problem on the resulting graph, and can thus be solved in linear time. In practice, this theoretical guarantee translates into assemblies of higher quality. Evaluations on both simulated reads from real genomes and a PacBio Escherichia coli K12 dataset demonstrate that Not-So-Greedy compares favorably with standard string graph approaches in terms of accuracy of the resulting read-overlap graph and contig N50. Available at github.com/samhykim/nsg courtade@eecs.berkeley.edu or dntse@stanford.edu Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Different architectures in the assembly of infectious bursal disease virus capsid proteins expressed in insect cells.

PubMed

Martinez-Torrecuadrada, J L; Castón, J R; Castro, M; Carrascosa, J L; Rodriguez, J F; Casal, J I

2000-12-20

Infectious bursal disease virus (IBDV) capsid is formed by the processing of a large polyprotein and subsequent assembly of VPX/VP2 and VP3. To learn more about the processing of the polyprotein and factors affecting the correct assembly of the viral capsid in vitro, different constructs were made using two baculovirus transfer vectors, pFastBac and pAcYM1. Surprisingly, the expression of the capsid proteins gave rise to different types of particles in each system, as observed by electron microscopy and immunofluorescence. FastBac expression led to the production of only rigid tubular structures, similar to those described as type I in viral infection. Western blot analysis revealed that these rigid tubules are formed exclusively by VPX. These tubules revealed a hexagonal arrangement of units that are trimer clustered, similar to those observed in IBDV virions. In contrast, pAcYM1 expression led to the assembly of virus-like particles (VLPs), flexible tubules, and intermediate assembly products formed by icosahedral caps elongated in tubes, suggesting an aberrant morphogenesis. Processing of VPX to VP2 seems to be a crucial requirement for the proper morphogenesis and assembly of IBDV particles. After immunoelectron microscopy, VPX/VP2 was detected on the surface of tubules and VLPs. We also demonstrated that VP3 is found only on the inner surfaces of VLPs and caps of the tubular structures. In summary, assembly of VLPs requires the internal scaffolding of VP3, which seems to induce the closing of the tubular architecture into VLPs and, thereafter, the subsequent processing of VPX to VP2. Copyright 2000 Academic Press.

Self-assembly of active amphiphilic Janus particles

NASA Astrophysics Data System (ADS)

Mallory, S. A.; Alarcon, F.; Cacciuto, A.; Valeriani, C.

2017-12-01

In this article, we study the phenomenology of a two dimensional dilute suspension of active amphiphilic Janus particles. We analyze how the morphology of the aggregates emerging from their self-assembly depends on the strength and the direction of the active forces. We systematically explore and contrast the phenomenologies resulting from particles with a range of attractive patch coverages. Finally, we illustrate how the geometry of the colloids and the directionality of their interactions can be used to control the physical properties of the assembled active aggregates and suggest possible strategies to exploit self-propulsion as a tunable driving force for self-assembly.

Hierarchical charge distribution controls self-assembly process of silk in vitro

NASA Astrophysics Data System (ADS)

Zhang, Yi; Zhang, Cencen; Liu, Lijie; Kaplan, David L.; Zhu, Hesun; Lu, Qiang

2015-12-01

Silk materials with different nanostructures have been developed without the understanding of the inherent transformation mechanism. Here we attempt to reveal the conversion road of the various nanostructures and determine the critical regulating factors. The regulating conversion processes influenced by a hierarchical charge distribution were investigated, showing different transformations between molecules, nanoparticles and nanofibers. Various repulsion and compressive forces existed among silk fibroin molecules and aggregates due to the exterior and interior distribution of charge, which further controlled their aggregating and deaggregating behaviors and finally formed nanofibers with different sizes. Synergistic action derived from molecular mobility and concentrations could also tune the assembly process and final nanostructures. It is suggested that the complicated silk fibroin assembly processes comply a same rule based on charge distribution, offering a promising way to develop silk-based materials with designed nanostructures.

MetaCAA: A clustering-aided methodology for efficient assembly of metagenomic datasets.

PubMed

Reddy, Rachamalla Maheedhar; Mohammed, Monzoorul Haque; Mande, Sharmila S

2014-01-01

A key challenge in analyzing metagenomics data pertains to assembly of sequenced DNA fragments (i.e. reads) originating from various microbes in a given environmental sample. Several existing methodologies can assemble reads originating from a single genome. However, these methodologies cannot be applied for efficient assembly of metagenomic sequence datasets. In this study, we present MetaCAA - a clustering-aided methodology which helps in improving the quality of metagenomic sequence assembly. MetaCAA initially groups sequences constituting a given metagenome into smaller clusters. Subsequently, sequences in each cluster are independently assembled using CAP3, an existing single genome assembly program. Contigs formed in each of the clusters along with the unassembled reads are then subjected to another round of assembly for generating the final set of contigs. Validation using simulated and real-world metagenomic datasets indicates that MetaCAA aids in improving the overall quality of assembly. A software implementation of MetaCAA is available at https://metagenomics.atc.tcs.com/MetaCAA. Copyright © 2014 Elsevier Inc. All rights reserved.

Effect of Structure on Physical Properties of Polymers.

DTIC Science & Technology

1979-12-31

PORT NUMBE . J ! 2. GOVT ACCESSION NO. 3. RECIPIENT’S CATALOG NUMBER OSRT R’.-8 00 7 5 0 4_7_5_ Effecc of Structure on Physical Properties of -Final...Compatibility of Fluorosubstituted Styrene Polymers with PPO and PS. R. Vukovic , F.E. Karasz, W.J. MacKnight, (in press). (6) Compatibility of Ortho- and Para...fluorostyrene Copolymers with PPO and PS. R. Vukovic , F.E. Karasz, W.J. MacKnight, (in press). (7) Partial Miscibility in the System Poly (para

STS_135_ MEDIA

NASA Image and Video Library

2011-06-30

JSC2011-E-060794 (30 June 2011) --- NASA astronaut Chris Ferguson, STS-135 commander, points to acknowledge a reporter while greeting the media along with NASA astronauts Doug Hurley, pilot, and Sandy Magnus and Rex Walheim, both mission specialists, during the STS-135 crew media briefing at NASA?s Johnson Space Center June 30, 2011. The press conference provided the last scheduled opportunity for a large group of press to speak with the crew before the final launch on July 8. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

STS_135_ MEDIA

NASA Image and Video Library

2011-06-30

JSC2011-E-060798 (30 June 2011) --- The STS-135 crew, from left, mission specialists Sandy Magnus and Rex Walheim, commander Chris Ferguson and pilot Doug Hurley follow Public Affairs Officer Nicole Cloutier-Lemasters to begin a crew media briefing at NASA?s Johnson Space Center on June 30, 2011. The press conference provided the last scheduled opportunity for a large group of press to speak with the crew before the final launch on July 8. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

Combat Stories: Creating a Web-Based Geospatial Interface to Record Combat Stories for Validation and Other Research Purposes

DTIC Science & Technology

2015-06-01

experiences of Odysseus on his way home. The final example is the Hebrew Bible that is composed from Old Testament stories, which were written by the third...19. 7 John Riches, The Bible : A Very Short Introduction (Oxford: Oxford University Press, 2000), 37. 8 Thomas G. Bowie, Jr., “An I for an Eye...Riches, John. The Bible : A Very Short Introduction. Oxford: Oxford University Press, 2000. 56 Sanchez, Ricardo S. with Donald T. Phillips. Wiser in

Status of the Regenerative ECLSS Water Recovery System

NASA Technical Reports Server (NTRS)

Carter, Donald Layne

2009-01-01

NASA has completed the delivery of the regenerative Water Recovery System (WRS) for the International Space Station (ISS). The major assemblies included in this system are the Water Processor Assembly (WPA) and Urine Processor Assembly (UPA). This paper summarizes the final effort to deliver the hardware to the Kennedy Space Center for launch on STS-126, the on-orbit status as of April 2009, and describes some of the technical challenges encountered and lessons learned over the past year.

Development of environmentally conscious cleaning process for leadless chip carrier assemblies. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adams, B.E.

1995-04-01

A cross-functional team of process, product, quality, material, and design lab engineers was assembled to develop an environmentally friendly cleaning process for leadless chip carrier assemblies (LCCAs). Using flush and filter testing, Auger surface analysis, GC-Mass spectrophotometry, production yield results, and electrical testing results over an extended testing period, the team developed an aqueous cleaning process for LCCAs. The aqueous process replaced the Freon vapor degreasing/ultrasonic rinse process.

Ionic Self-Assembled Monolayer (ISAM) Nonlinear Optical Thin Films and Devices

DTIC Science & Technology

1998-05-12

SUBTITLE " Ionic Self-Assembled Monolayer (ISAM) Nonlinear Optical Thin Films and Devices" 6. AUTHORS Michael B. Miller 5. FUNDING NUMBERS F49620-97...ii. Lü. Ionic Self-Assembled Monolayer (ISAM) Nonlinear Optical Thin Films and Devices Final Technical Report Performance Period: 15 August 1997...Investigator F&S. Inc.N ̂ 1. INTRODUCTION .’ 2 2. PROGRAM TASK REVIEW 2 3. BACKGROUND 4 3.1 NONLINEAR OPTICAL THIN FILMS 4 3.2 IONIC SELF

The NatCarb geoportal: Linking distributed data from the Carbon Sequestration Regional Partnerships

USGS Publications Warehouse

Carr, T.R.; Rich, P.M.; Bartley, J.D.

2007-01-01

The Department of Energy (DOE) Carbon Sequestration Regional Partnerships are generating the data for a "carbon atlas" of key geospatial data (carbon sources, potential sinks, etc.) required for rapid implementation of carbon sequestration on a broad scale. The NATional CARBon Sequestration Database and Geographic Information System (NatCarb) provides Web-based, nation-wide data access. Distributed computing solutions link partnerships and other publicly accessible repositories of geological, geophysical, natural resource, infrastructure, and environmental data. Data are maintained and enhanced locally, but assembled and accessed through a single geoportal. NatCarb, as a first attempt at a national carbon cyberinfrastructure (NCCI), assembles the data required to address technical and policy challenges of carbon capture and storage. We present a path forward to design and implement a comprehensive and successful NCCI. ?? 2007 The Haworth Press, Inc. All rights reserved.

Aerial view of Launch Complex 39

NASA Technical Reports Server (NTRS)

1998-01-01

In this aerial view looking south can be seen Launch Complex (LC) 39 area, where assembly, checkout and launch of the Space Shuttle Orbiter and its External Tank and twin Solid Rocket Boosters take place. Central to the complex is the tallest building at the center, the Vehicle Assembly Building (VAB). To the immediate left, from top to bottom, are the Orbiter Processing Facility (OPF) High Bay 3 and new engine shop (north side), OPF Modular Office Building, Thermal Protection System Facility, and a crawler-transporter (to its left). In front of the VAB are OPF 1 and OPF 2. At right is the Processing Control Center. West of OPF 3 is the Mobile Launch Platform. In the upper left corner is Launch Pad B; at the far right is the turn basin, with the Press Site located just below it to the right.

On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker.

PubMed

Lai, Yen-Ting; Jiang, Lin; Chen, Wuyang; Yeates, Todd O

2015-11-01

Connecting proteins together in prescribed geometric arrangements is an important element in new areas of biomolecular design. In this study, we characterize the degree of three-dimensional orientational control that can be achieved when two protein domains that have alpha-helical termini are joined using an alpha-helical linker. A fusion between naturally oligomeric protein domains was designed in this fashion with the intent of creating a self-assembling 12-subunit tetrahedral protein cage. While the designed fusion protein failed to assemble into a tetrahedral cage in high yield, a series of crystal structures showed that the two fused components were indeed bridged by an intact alpha helix, although the fusion protein was distorted from the intended ideal configuration by bending of the helix, ranging from 7 to 35°. That range of deviation in orientation creates challenges for designing large, perfectly symmetric protein assemblies, although it should offer useful outcomes for other less geometrically demanding applications in synthetic biology. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Self-assembly of [Pt(3n)(CO)(6n)]2- (n = 4-8) carbonyl clusters: from molecules to conducting molecular metal wires.

PubMed

Femoni, Cristina; Iapalucci, Maria Carmela; Longoni, Giuliano; Lovato, Tatiana; Stagni, Stefano; Zacchini, Stefano

2010-07-05

A comprehensive study discussing the different parameters that influence the self-assembly of [Pt(3n)(CO)(6n)](2-) (n = 4-8) clusters with miscellaneous mono- and dications into 0-D, 1-D, 2-D, and 3-D materials is herein reported. As an unexpected bonus, the use of Ru(II) dications allowed the first structural characterization of the previously unknown [Pt(21)(CO)(42)](2-) dianion. 0-D structures, which contain isolated ions, are electrical insulators in solid form. Conversely, as soon as infinite chains of clusters are formed, the electrical resistivity, measured in pressed pellets, decreases to 10(5)-10(6), 10(4), and 10(2) ohms cm for discontinuous, semicontinuous, and continuous chains, respectively. Therefore, the resemblance of these materials to molecular metal wires is not only morphological but also functional. Preliminary results of possible self-assembly phenomena in a solution of [Pt(15)(CO)(30)](2-) and [Pt(18)(CO)(36)](2-) according to dynamic light scattering experiments are also reported.

Partial bisulfite conversion for unique template sequencing.

PubMed

Kumar, Vijay; Rosenbaum, Julie; Wang, Zihua; Forcier, Talitha; Ronemus, Michael; Wigler, Michael; Levy, Dan

2018-01-25

We introduce a new protocol, mutational sequencing or muSeq, which uses sodium bisulfite to randomly deaminate unmethylated cytosines at a fixed and tunable rate. The muSeq protocol marks each initial template molecule with a unique mutation signature that is present in every copy of the template, and in every fragmented copy of a copy. In the sequenced read data, this signature is observed as a unique pattern of C-to-T or G-to-A nucleotide conversions. Clustering reads with the same conversion pattern enables accurate count and long-range assembly of initial template molecules from short-read sequence data. We explore count and low-error sequencing by profiling 135 000 restriction fragments in a PstI representation, demonstrating that muSeq improves copy number inference and significantly reduces sporadic sequencer error. We explore long-range assembly in the context of cDNA, generating contiguous transcript clusters greater than 3,000 bp in length. The muSeq assemblies reveal transcriptional diversity not observable from short-read data alone. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Autonomous assembly with collision avoidance of a fleet of flexible spacecraft based on disturbance observer

NASA Astrophysics Data System (ADS)

Chen, Ti; Wen, Hao

2018-06-01

This paper presents a distributed control law with disturbance observer for the autonomous assembly of a fleet of flexible spacecraft to construct a large flexible space structure. The fleet of flexible spacecraft is driven to the pre-assembly configuration firstly, and then to the desired assembly configuration. A distributed assembly control law with disturbance observer is proposed by treating the flexible dynamics as disturbances acting on the rigid motion of the flexible spacecraft. Theoretical analysis shows that the control law can actuate the fleet to the desired configuration. Moreover, the collision avoidance between the members is also considered in the process from initial configuration to pre-assembly configuration. Finally, a numerical example is presented to verify the feasibility of proposed mission planning and the effectiveness of control law.

Clinical and radiographic outcomes after total shoulder arthroplasty with an anatomic press-fit short stem.

PubMed

Szerlip, Benjamin W; Morris, Brent J; Laughlin, Mitzi S; Kilian, Christopher M; Edwards, T Bradley

2018-01-01

There is limited information assessing outcomes after total shoulder arthroplasty (TSA) with a press-fit short stem. The purpose of this study was to evaluate early clinical and radiographic outcomes and complications after TSA with an anatomic press-fit short stem. We identified 118 TSAs completed with press-fit short stem and minimum 2-year follow-up; 85 of these patients had a grit-blasted (GB) short stem placed, whereas 33 patients had short stems with proximal porous coating (PPC). Shoulder function scores, active mobility measurements, and radiographs were assessed. The average age at surgery was 66.7 years, with average follow-up of 3 years. Significant improvements were noted for all shoulder function scores and active mobility measurements from the preoperative state to final follow-up (P < .001). There was no stem loosening in any patients with minimum 2-year follow-up; however, 3 female patients with GB stems had gross loosening of their humeral components before 1 year, 2 requiring revision. Radiolucent lines around the humeral component were found in 5.9% without evidence of loosening. Osseous resorption at the medial cortex was noted in 9.3%. Of patients with PPC stems, no patients were observed to have radiolucent lines compared with 8.2% in the GB group (P = .09). TSAs with anatomic press-fit short stem showed significant clinical improvements from the preoperative state to final short-term follow-up, with few complications and minimal radiographic changes. Lack of PPC may contribute to early loosening in patients with poor bone quality. The authors now use a short stem with PPC. Copyright © 2017 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.

Ethical and policy issues in rehabilitation medicine.

PubMed

Caplan, Arthur L; Callahan, Daniel; Haas, Janet

1987-08-01

The field of medical rehabilitation is relatively new.... Until recently, the ethical problems of this new field were neglected. There seemed to be more pressing concerns as rehabilitation medicine struggled to establish itself, sometimes in the face of considerable skepticism or hostility. There also seemed no pressing moral questions of the kind and intensity to be encountered, say, in high-technology acute care medicine or genetic engineering.... Those in biomedical ethics could and did easily overlook the quiet, less obtrusive issues of rehabilitation.... The Hastings Center set out in 1985 to rectify that situation.... To explore the issues, the Center assembled a group of practitioners in the field, Hastings Center staff members, and individuals experienced in other areas of medical ethics.... The report that follows was written by Arthur Caplan and Daniel Callahan, assisted by Dr. Janet Haas of the Moss Rehabilitation Hospital in Philadelphia....

KSC-2010-4579

NASA Image and Video Library

2010-09-08

CAPE CANAVERAL, Fla. -- In the LC-39 Complex Turn Basin area across from the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, a major water main leak in a 24-inch pipe caused soil to wash away near the Press Site. The center was closed for the morning while workers assessed and repaired the break. In the background is the Pegasus barge docked at the Turn Basin which is used to deliver the space shuttle external fuel tank. Photo credit: NASA/Jack Pfaller

KSC-2010-4582

NASA Image and Video Library

2010-09-08

CAPE CANAVERAL, Fla. -- In the LC-39 Complex Turn Basin area across from the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, a major water main leak in a 24-inch pipe caused soil to wash away near the Press Site. The center was closed for the morning while workers assessed and repaired the break. In the background is the Pegasus barge docked at the Turn Basin which is used to deliver the space shuttle external fuel tank. Photo credit: NASA/Jack Pfaller

KSC-2010-4580

NASA Image and Video Library

2010-09-08

CAPE CANAVERAL, Fla. -- In the LC-39 Complex Turn Basin area across from the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, a major water main leak in a 24-inch pipe caused soil to wash away near the Press Site. The center was closed for the morning while workers assessed and repaired the break. In the background is the Pegasus barge docked at the Turn Basin which is used to deliver the space shuttle external fuel tank. Photo credit: NASA/Jack Pfaller

03pd2227

NASA Image and Video Library

2003-07-23

KENNEDY SPACE CENTER, FLA. – This view shows much of the Launch Complex 39 Area stretching beyond the Turn Basin in the foreground. The largest building is the 525-foot-tall Vehicle Assembly Building. In front of it is the Launch Control Center. Behind and to the left of the VAB are the Orbiter Processing Facility bays. At left are the Multi-Function Facility and Operations Support Building. At left of the Turn Basin is the Press Site, which comprises the NASA News Center, grandstand, TV studio and media buildings.

Biotechnology Predictors of Physical Security Personnel Performance. I. A Review of the Stress Literature Related to Performance.

DTIC Science & Technology

1983-06-01

Payne (Eds.) Stress at work. New York: John Wiley, 1978. Broadbent, D. E. Decision and stress. New York: Academic Press, 1971. Bryden, M. P. Binaural ...to satisfaction [emphasis is the author’s]. Bryden, M. P. Binaural competition and division of attention as determi- nants of the laterality effect in...invasively. This system has been used in an industrial setting to monitor workers on an assembly line. The method of obtaining heart rate, it’s beat

α-Conotoxin MII-Sensitive Nicotinic Acetylcholine Receptors in the Nucleus Accumbens Shell Regulate Progressive Ratio Responding Maintained by Nicotine

PubMed Central

Brunzell, Darlene H; Boschen, Karen E; Hendrick, Elizabeth S; Beardsley, Patrick M; McIntosh, J Michael

2010-01-01

β2 subunit containing nicotinic acetylcholine receptors (β2*nAChRs; asterisk (*) denotes assembly with other subunits) are critical for nicotine self-administration and nicotine-associated dopamine (DA) release that supports nicotine reinforcement. The α6 subunit assembles with β2 on DA neurons where α6β2*nAChRs regulate nicotine-stimulated DA release at neuron terminals. Using local infusion of α-conotoxin MII (α-CTX MII), an antagonist with selectivity for α6β2*nAChRs, the purpose of these experiments was to determine if α6β2*nAChRs in the nucleus accumbens (NAc) shell are required for motivation to self-administer nicotine. Long-Evans rats lever-pressed for 0.03 mg/kg, i.v., nicotine accompanied by light+tone cues (NIC) or for light+tone cues unaccompanied by nicotine (CUEonly). Following extensive training, animals were tested under a progressive ratio (PR) schedule that required an increasing number of lever presses for each nicotine infusion and/or cue delivery. Immediately before each PR session, rats received microinfusions of α-CTX MII (0, 1, 5, or 10 pmol per side) into the NAc shell or the overlying anterior cingulate cortex. α-CTX MII dose dependently decreased break points and number of infusions earned by NIC rats following infusion into the NAc shell but not the anterior cingulate cortex. Concentrations of α-CTX MII that were capable of attenuating nicotine self-administration did not disrupt locomotor activity. There was no effect of infusion on lever pressing in CUEonly animals and NAc infusion α-CTX MII did not affect locomotor activity in an open field. These data suggest that α6β2*nAChRs in the NAc shell regulate motivational aspects of nicotine reinforcement but not nicotine-associated locomotor activation. PMID:19890263

High pressure low temperature hot pressing method for producing a zirconium carbide ceramic

DOEpatents

Cockeram, Brian V.

2017-01-10

A method for producing monolithic Zirconium Carbide (ZrC) is described. The method includes raising a pressure applied to a ZrC powder until a final pressure of greater than 40 MPa is reached; and raising a temperature of the ZrC powder until a final temperature of less than 2200.degree. C. is reached.

World Organisation for Animal Health

MedlinePlus

... Our members Delegates Key texts Basic texts Cooperation agreements Official Acts Resolutions and recommendations Final Reports of ... information: press releases, fact sheets, infographics… Partners Collaboration agreements with regional and world organisations of a public ...

Proposal for the completion of outstanding work on the installation scheduling and alignment of the SDC central calorimeter

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guarino, V.; Hill, N.; Nasiatka, J.

The High Energy Physic Division at Argonne National Laboratory was given the task of developing the procedures, fixtures, and schedules for the final assembly of the barrel and endcap calorimeters for the SDC. The work completed led to some major decision about how and where this assembly work would be done. The primary assembly decision was the feasibility of assembling the major detector components (barrel and endcap sub-assemblies) above ground and lowering them into position in the experimental hall, as opposed to assembling the calorimeter directly in the experimental hall. Due to cost of above ground assembly and schedule changes,more » the in-hall option was adopted. Although no actual hardware was constructed, many conceptual ideas were formalized and brought to workable solutions as a result of the effort put forth at ANL.« less

Pathways for virus assembly around nucleic acids

PubMed Central

Perlmutter, Jason D; Perkett, Matthew R

2014-01-01

Understanding the pathways by which viral capsid proteins assemble around their genomes could identify key intermediates as potential drug targets. In this work we use computer simulations to characterize assembly over a wide range of capsid protein-protein interaction strengths and solution ionic strengths. We find that assembly pathways can be categorized into two classes, in which intermediates are either predominantly ordered or disordered. Our results suggest that estimating the protein-protein and the protein-genome binding affinities may be sufficient to predict which pathway occurs. Furthermore, the calculated phase diagrams suggest that knowledge of the dominant assembly pathway and its relationship to control parameters could identify optimal strategies to thwart or redirect assembly to block infection. Finally, analysis of simulation trajectories suggests that the two classes of assembly pathways can be distinguished in single molecule fluorescence correlation spectroscopy or bulk time resolved small angle x-ray scattering experiments. PMID:25036288

76 FR 72440 - Bottom Mount Combination Refrigerator-Freezers From Korea and Mexico; Scheduling of the Final...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-23

... refrigeration using compression technology, with all of the following characteristics: (1) The cabinet contains... interior plastic liner, (e) wiring, and (f) insulation; (2) any assembled external doors designed for use... metal shell, (b) an interior plastic liner, and (c) insulation; and (3) any assembled external drawers...

Improvements of self-assembly properties via homopolymer addition or block-copolymer blends

NASA Astrophysics Data System (ADS)

Chevalier, X.; Nicolet, C.; Tiron, R.; Gharbi, Ahmed; Argoud, M.; Couderc, C.; Fleury, Guillaume; Hadziioannou, G.; Iliopoulos, I.; Navarro, C.

2014-03-01

The properties of cylindrical poly(styrene-b-methylmethacrylate) (PS-b-PMMA) BCPs self-assembly in thinfilms are studied when the pure BCPs are blended either with a homopolymer or with another cylindrical PS-b-PMMA based BCP. For both of these approaches, we show that the period of the self-assembled features can be easily tuned and controlled, and that the final material presents interesting characteristics, such as the possibility to achieve thicker defects-free films, as compared to pure block-copolymers having the same period. Moreover, a statistical defectivity study based on a Delaunay triangulation and Voronoi analysis of the self-assemblies made with the different blends is described, and prove that despite their high value of polydispersity index, these blends exhibit also improved selfassembly properties (bigger monocrystalline arrangements and enhanced kinetics of defects annihilation) as compared to pure and monodisperse block-copolymers. Finally, the behavior of the blends is also compared to the ones their pure counter-part in templated approach like the contact-hole shrink to evaluate their respective process-window and response toward this physical constrain for lithographic applications.

Minimal Absent Words in Four Human Genome Assemblies

PubMed Central

Garcia, Sara P.; Pinho, Armando J.

2011-01-01

Minimal absent words have been computed in genomes of organisms from all domains of life. Here, we aim to contribute to the catalogue of human genomic variation by investigating the variation in number and content of minimal absent words within a species, using four human genome assemblies. We compare the reference human genome GRCh37 assembly, the HuRef assembly of the genome of Craig Venter, the NA12878 assembly from cell line GM12878, and the YH assembly of the genome of a Han Chinese individual. We find the variation in number and content of minimal absent words between assemblies more significant for large and very large minimal absent words, where the biases of sequencing and assembly methodologies become more pronounced. Moreover, we find generally greater similarity between the human genome assemblies sequenced with capillary-based technologies (GRCh37 and HuRef) than between the human genome assemblies sequenced with massively parallel technologies (NA12878 and YH). Finally, as expected, we find the overall variation in number and content of minimal absent words within a species to be generally smaller than the variation between species. PMID:22220210

Mechanisms of nuclear pore complex assembly - two different ways of building one molecular machine.

PubMed

Otsuka, Shotaro; Ellenberg, Jan

2018-02-01

The nuclear pore complex (NPC) mediates all macromolecular transport across the nuclear envelope. In higher eukaryotes that have an open mitosis, NPCs assemble at two points in the cell cycle: during nuclear assembly in late mitosis and during nuclear growth in interphase. How the NPC, the largest nonpolymeric protein complex in eukaryotic cells, self-assembles inside cells remained unclear. Recent studies have started to uncover the assembly process, and evidence has been accumulating that postmitotic and interphase NPC assembly use fundamentally different mechanisms; the duration, structural intermediates, and regulation by molecular players are different and different types of membrane deformation are involved. In this Review, we summarize the current understanding of these two modes of NPC assembly and discuss the structural and regulatory steps that might drive the assembly processes. We furthermore integrate understanding of NPC assembly with the mechanisms for rapid nuclear growth in embryos and, finally, speculate on the evolutionary origin of the NPC implied by the presence of two distinct assembly mechanisms. © 2017 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

Comparing memory-efficient genome assemblers on stand-alone and cloud infrastructures.

PubMed

Kleftogiannis, Dimitrios; Kalnis, Panos; Bajic, Vladimir B

2013-01-01

A fundamental problem in bioinformatics is genome assembly. Next-generation sequencing (NGS) technologies produce large volumes of fragmented genome reads, which require large amounts of memory to assemble the complete genome efficiently. With recent improvements in DNA sequencing technologies, it is expected that the memory footprint required for the assembly process will increase dramatically and will emerge as a limiting factor in processing widely available NGS-generated reads. In this report, we compare current memory-efficient techniques for genome assembly with respect to quality, memory consumption and execution time. Our experiments prove that it is possible to generate draft assemblies of reasonable quality on conventional multi-purpose computers with very limited available memory by choosing suitable assembly methods. Our study reveals the minimum memory requirements for different assembly programs even when data volume exceeds memory capacity by orders of magnitude. By combining existing methodologies, we propose two general assembly strategies that can improve short-read assembly approaches and result in reduction of the memory footprint. Finally, we discuss the possibility of utilizing cloud infrastructures for genome assembly and we comment on some findings regarding suitable computational resources for assembly.

KSC-2010-4885

NASA Image and Video Library

2010-09-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, Bill McArthur, (left) Space Shuttle Program Orbiter Projects manager; John Casper, Assistant Space Shuttle Program manager; John Shannon, Space Shuttle Program manager and Canadian Space Agency astronaut Chris Hadfield attend a ceremony being held to commemorate the move from Kennedy's Assembly Refurbishment Facility (ARF) to the Vehicle Assembly Building (VAB) of the Space Shuttle Program's final solid rocket booster structural assembly -- the right-hand forward. The move was postponed because of inclement weather. Photo credit: NASA/Kim Shiflett

Hybrid Wing Body Multi-Bay Test Article Analysis and Assembly Final Report

NASA Technical Reports Server (NTRS)

Velicki, Alexander; Hoffman, Krishna; Linton, Kim A.; Baraja, Jaime; Wu, Hsi-Yung T.; Thrash, Patrick

2017-01-01

This report summarizes work performed by The Boeing Company, through its Boeing Research & Technology organization located in Huntington Beach, California, under the Environmentally Responsible Aviation (ERA) project. The report documents work performed to structurally analyze and assemble a large-scale Multi-bay Box (MBB) Test Article capable of withstanding bending and internal pressure loadings representative of a Hybrid Wing Body (HWB) aircraft. The work included fabrication of tooling elements for use in the fabrication and assembly of the test article.

Thermal-induced phase transition and assembly of hexagonal metastable In 2O 3 nanocrystals: A new approach to In 2O 3 functional materials

NASA Astrophysics Data System (ADS)

Shu, Shiwen; Yu, Dabin; Wang, Yan; Wang, Feng; Wang, Zirong; Zhong, Wu

2010-10-01

This paper reports on the thermal-induced performance of hexagonal metastable In 2O 3 nanocrystals involving in phase transition and assembly, with particular emphasis on the assembly for the preparation of functional materials. For In 2O 3 nanocrystals, the metastable phase was found to be thermally unstable and transform to cubic phase when temperature was higher than 600 °C, accompanied by assembly as well as evolution of optical properties, but the two polymorphs coexisted at the temperature ranging from 600 to 900 °C, during which the content of product phase and crystal size gradually increased upon increasing temperature. The assembly of In 2O 3 nanocrystals can be developed to fabricate In 2O 3 functional materials, such as various ceramic materials, or even desired nano- or micro-structures, by using metastable In 2O 3 nanocrystals as precursors or building blocks. The electrical resistivity of In 2O 3 conductive film fabricated by a hot-pressing route was as low as 3.72×10 -3 Ω cm, close to that of In 2O 3 single crystal, which is important for In 2O 3 that is always used as conductive materials. The findings should be of importance for both the wide applications of In 2O 3 in optical and electronic devices and theoretical investigations on crystal structures.

White-tailed deer are a biotic filter during community assembly, reducing species and phylogenetic diversity.

PubMed

Begley-Miller, Danielle R; Hipp, Andrew L; Brown, Bethany H; Hahn, Marlene; Rooney, Thomas P

2014-06-09

Community assembly entails a filtering process, where species found in a local community are those that can pass through environmental (abiotic) and biotic filters and successfully compete. Previous research has demonstrated the ability of white-tailed deer (Odocoileus virginianus) to reduce species diversity and favour browse-tolerant plant communities. In this study, we expand on our previous work by investigating deer as a possible biotic filter altering local plant community assembly. We used replicated 23-year-old deer exclosures to experimentally assess the effects of deer on species diversity (H'), richness (SR), phylogenetic community structure and phylogenetic diversity in paired browsed (control) and unbrowsed (exclosed) plots. Additionally, we developed a deer-browsing susceptibility index (DBSI) to assess the vulnerability of local species to deer. Deer browsing caused a 12 % reduction in H' and 17 % reduction in SR, consistent with previous studies. Furthermore, browsing reduced phylogenetic diversity by 63 %, causing significant phylogenetic clustering. Overall, graminoids were the least vulnerable to deer browsing based on DBSI calculations. These findings demonstrate that deer are a significant driver of plant community assembly due to their role as a selective browser, or more generally, as a biotic filter. This study highlights the importance of knowledge about the plant tree of life in assessing the effects of biotic filters on plant communities. Application of such knowledge has considerable potential to advance our understanding of plant community assembly. Published by Oxford University Press on behalf of the Annals of Botany Company.

Performance and microbial ecology of air-cathode microbial fuel cells with layered electrode assemblies.

PubMed

Butler, Caitlyn S; Nerenberg, Robert

2010-05-01

Microbial fuel cells (MFCs) can be built with layered electrode assemblies, where the anode, proton exchange membrane (PEM), and cathode are pressed into a single unit. We studied the performance and microbial community structure of MFCs with layered assemblies, addressing the effect of materials and oxygen crossover on the community structure. Four MFCs with layered assemblies were constructed using Nafion or Ultrex PEMs and a plain carbon cloth electrode or a cathode with an oxygen-resistant polytetrafluoroethylene diffusion layer. The MFC with Nafion PEM and cathode diffusion layer achieved the highest power density, 381 mW/m(2) (20 W/m(3)). The rates of oxygen diffusion from cathode to anode were three times higher in the MFCs with plain cathodes compared to those with diffusion-layer cathodes. Microsensor studies revealed little accumulation of oxygen within the anode cloth. However, the abundance of bacteria known to use oxygen as an electron acceptor, but not known to have exoelectrogenic activity, was greater in MFCs with plain cathodes. The MFCs with diffusion-layer cathodes had high abundance of exoelectrogenic bacteria within the genus Geobacter. This work suggests that cathode materials can significantly influence oxygen crossover and the relative abundance of exoelectrogenic bacteria on the anode, while PEM materials have little influence on anode community structure. Our results show that oxygen crossover can significantly decrease the performance of air-cathode MFCs with layered assemblies, and therefore limiting crossover may be of particular importance for these types of MFCs.

Deconstructing Building Blocks: Preschoolers' Spatial Assembly Performance Relates to Early Mathematics Skills

PubMed Central

Verdine, Brian N.; Golinkoff, Roberta Michnick; Hirsh-Pasek, Kathryn; Newcombe, Nora S.; Filipowicz, Andrew T.; Chang, Alicia

2013-01-01

This study focuses on three main goals: First, 3-year-olds' spatial assembly skills are probed using interlocking block constructions (N = 102). A detailed scoring scheme provides insight into early spatial processing and offers information beyond a basic accuracy score. Second, the relation of spatial assembly to early mathematics skills was evaluated. Spatial skill independently predicted a significant amount of the variability in concurrent mathematics performance. Finally, the relationship between spatial assembly skill and socioeconomic status, gender, and parent-reported spatial language was examined. While children's performance did not differ by gender, lower-SES children were already lagging behind higher-SES children in block assembly. Furthermore, lower-SES parents reported using significantly fewer spatial words with their children. PMID:24112041

Tools and Equipment Modeling for Automobile Interactive Assembling Operating Simulation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu Dianliang; Zhu Hongmin; Shanghai Key Laboratory of Advance Manufacturing Environment

Tools and equipment play an important role in the simulation of virtual assembly, especially in the assembly process simulation and plan. Because of variety in function and complexity in structure and manipulation, the simulation of tools and equipments remains to be a challenge for interactive assembly operation. Based on analysis of details and characteristics of interactive operations for automobile assembly, the functional requirement for tools and equipments of automobile assembly is given. Then, a unified modeling method for information expression and function realization of general tools and equipments is represented, and the handling methods of manual, semi-automatic, automatic tools andmore » equipments are discussed. Finally, the application in assembly simulation of rear suspension and front suspension of Roewe 750 automobile is given. The result shows that the modeling and handling methods are applicable in the interactive simulation of various tools and equipments, and can also be used for supporting assembly process planning in virtual environment.« less

Two men with multiple disabilities carry out an assembly work activity with the support of a technology system.

PubMed

Lancioni, Giulio E; Singh, Nirbhay N; O'Reilly, Mark F; Green, Vanessa A; Oliva, Doretta; Campodonico, Francesca

2013-10-01

To assess whether two persons with multiple disabilities could learn a work activity (i.e., assembling trolley wheels) with the support of a technology system. After an initial baseline, the study compared the effects of intervention sessions relying on the technology system (which called the participants to the different workstations and provided feedback and final stimulation) with the effects of intervention sessions carried out without technology. The two types of intervention sessions were conducted according to an alternating treatments design. Eventually, only intervention sessions relying on the technology system were used. Both participants managed to assemble wheels independently during intervention sessions relying on the technology system while they failed during sessions without the system. Their performance was strengthened during the final part of the study, in which only sessions with the system occurred. Technology may be critical in helping persons with multiple disabilities manage multi-step work activities.

Pathway-engineering for highly-aligned block copolymer arrays.

PubMed

Choo, Youngwoo; Majewski, Paweł W; Fukuto, Masafumi; Osuji, Chinedum O; Yager, Kevin G

2017-12-21

While the ultimate driving force in self-assembly is energy minimization and the corresponding evolution towards equilibrium, kinetic effects can also play a very strong role. These kinetic effects, such as trapping in metastable states, slow coarsening kinetics, and pathway-dependent assembly, are often viewed as complications to be overcome. Here, we instead exploit these effects to engineer a desired final nano-structure in a block copolymer thin film, by selecting a particular ordering pathway through the self-assembly energy landscape. In particular, we combine photothermal shearing with high-temperature annealing to yield hexagonal arrays of block copolymer cylinders that are aligned in a single prescribed direction over macroscopic sample dimensions. Photothermal shearing is first used to generate a highly-aligned horizontal cylinder state, with subsequent thermal processing used to reorient the morphology to the vertical cylinder state in a templated manner. Finally, we demonstrate the successful transfer of engineered morphologies into inorganic replicas.

Effects of sintering time and temperature to the characteristics of FeCrAl powder compacts formed at elevated temperature

NASA Astrophysics Data System (ADS)

Rahman, M. M.; Rahman, H. Y.; Awang, M. A. A.; Sopyan, I.

2018-01-01

This paper presents the outcomes of an experimental investigation on the effect of sintering schedule, i.e., holding time and temperature to the final properties of FeCrAl powder compacts prepared through uniaxial die compaction process at above room temperature. The feedstock was prepared by mechanically mixing iron powder ASC 100.29 with chromium (22 wt%) and aluminium (11 wt%) for 30 min at room temperature. A cylindrical shape die was filled with the powder mass and heated for one hour for uniform heating of the die assembly together with the powder mass. Once the temperature reached to the setup temperature, i.e., 150°C, the powder mass was formed by applying an axial pressure of 425 MPa simultaneously from upward and downward directions. The as-pressed green compacts were then cooled to room temperature and subsequently sintered in argon gas fired furnace at a rate of 5°C/min for three different holding times, i.e., 30, 60, and 90 min at three different sintering temperatures, i.e., 800, 900, and 1000°C. The sintered samples were characterized for their density, electrical resistivity, bending strength, and microstructure. The results revealed that the sample sintered at 1000°C for 90 min achieved the better characteristics.

A short conserved motif in ALYREF directs cap- and EJC-dependent assembly of export complexes on spliced mRNAs.

PubMed

Gromadzka, Agnieszka M; Steckelberg, Anna-Lena; Singh, Kusum K; Hofmann, Kay; Gehring, Niels H

2016-03-18

The export of messenger RNAs (mRNAs) is the final of several nuclear posttranscriptional steps of gene expression. The formation of export-competent mRNPs involves the recruitment of export factors that are assumed to facilitate transport of the mature mRNAs. Using in vitro splicing assays, we show that a core set of export factors, including ALYREF, UAP56 and DDX39, readily associate with the spliced RNAs in an EJC (exon junction complex)- and cap-dependent manner. In order to elucidate how ALYREF and other export adaptors mediate mRNA export, we conducted a computational analysis and discovered four short, conserved, linear motifs present in RNA-binding proteins. We show that mutation in one of the new motifs (WxHD) in an unstructured region of ALYREF reduced RNA binding and abolished the interaction with eIF4A3 and CBP80. Additionally, the mutation impaired proper localization to nuclear speckles and export of a spliced reporter mRNA. Our results reveal important details of the orchestrated recruitment of export factors during the formation of export competent mRNPs. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC.

PubMed

Kenesi, Erzsébet; Carbonell, Alberto; Lózsa, Rita; Vértessy, Beáta; Lakatos, Lóránt

2017-07-27

In most eukaryotes, RNA silencing is an adaptive immune system regulating key biological processes including antiviral defense. To evade this response, viruses of plants, worms and insects have evolved viral suppressors of RNA silencing proteins (VSRs). Various VSRs, such as P1 from Sweet potato mild mottle virus (SPMMV), inhibit the activity of RNA-induced silencing complexes (RISCs) including an ARGONAUTE (AGO) protein loaded with a small RNA. However, the specific mechanisms explaining this class of inhibition are unknown. Here, we show that SPMMV P1 interacts with AGO1 and AGO2 from Arabidopsis thaliana, but solely interferes with AGO1 function. Moreover, a mutational analysis of a newly identified zinc finger domain in P1 revealed that this domain could represent an effector domain as it is required for P1 suppressor activity but not for AGO1 binding. Finally, a comparative analysis of the target RNA binding capacity of AGO1 in the presence of wild-type or suppressor-defective P1 forms revealed that P1 blocks target RNA binding to AGO1. Our results describe the negative regulation of RISC, the small RNA containing molecular machine. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Mars Observer Press Conference JPL

NASA Astrophysics Data System (ADS)

1993-08-01

The Mars Observer mission spacecraft was primarily designed for exploring Mars and the Martian environment. The Mars Observer was launched on September 25, 1992. The spacecraft was lost in the vicinity of Mars on August 21, 1993 when the spacecraft began its maneuvering sequence for Martian orbital insertion. This videotape shows a press briefing, held after the spacecraft had not responded to attempts to communicate with it, to explain to the press the problems and the steps that were being taken to re-establish communication with the spacecraft. The communications had been shutdown prior to the orbital insertion burn to protect the instruments. At the time of the press conference, the communications system was still not operational, and attempts were being made to re-establish communication. Bob McMillan of the Public Affairs Office at JPL gives the initial announcement of the continuing communication problem with the spacecraft. Mr. McMillan introduces William Piotrowski, acting director of solar system exploration, who reiterates that there is indeed no communication with the Observer spacecraft. He is followed by Glenn Cunningham, the Project Manager of the Mars Observer who speaks about the attempts to re-establish contact. Mr. Cunningham is followed by Satenios Dallas, the Mission Manager for the Mars Observer Project, who speaks about the sequence of events leading up to the communication failure, and shows an animated video presenting the orbital insertion maneuvers. The briefing was then opened up for questions from the assembled press, both at JPL and at the other NASA Centers. The questions are about the possible reasons for the communication failure, and the attempts to restore communications with the spacecraft. Dr. Arden L. Albee, chief scientist for the Mars Observer Mission, joins the other panel members to answer questions. At the end of the press briefing the animation of the Mars orbital insertion is shown again.

Extensive Error in the Number of Genes Inferred from Draft Genome Assemblies

PubMed Central

Denton, James F.; Lugo-Martinez, Jose; Tucker, Abraham E.; Schrider, Daniel R.; Warren, Wesley C.; Hahn, Matthew W.

2014-01-01

Current sequencing methods produce large amounts of data, but genome assemblies based on these data are often woefully incomplete. These incomplete and error-filled assemblies result in many annotation errors, especially in the number of genes present in a genome. In this paper we investigate the magnitude of the problem, both in terms of total gene number and the number of copies of genes in specific families. To do this, we compare multiple draft assemblies against higher-quality versions of the same genomes, using several new assemblies of the chicken genome based on both traditional and next-generation sequencing technologies, as well as published draft assemblies of chimpanzee. We find that upwards of 40% of all gene families are inferred to have the wrong number of genes in draft assemblies, and that these incorrect assemblies both add and subtract genes. Using simulated genome assemblies of Drosophila melanogaster, we find that the major cause of increased gene numbers in draft genomes is the fragmentation of genes onto multiple individual contigs. Finally, we demonstrate the usefulness of RNA-Seq in improving the gene annotation of draft assemblies, largely by connecting genes that have been fragmented in the assembly process. PMID:25474019

Extensive error in the number of genes inferred from draft genome assemblies.

PubMed

Denton, James F; Lugo-Martinez, Jose; Tucker, Abraham E; Schrider, Daniel R; Warren, Wesley C; Hahn, Matthew W

2014-12-01

Current sequencing methods produce large amounts of data, but genome assemblies based on these data are often woefully incomplete. These incomplete and error-filled assemblies result in many annotation errors, especially in the number of genes present in a genome. In this paper we investigate the magnitude of the problem, both in terms of total gene number and the number of copies of genes in specific families. To do this, we compare multiple draft assemblies against higher-quality versions of the same genomes, using several new assemblies of the chicken genome based on both traditional and next-generation sequencing technologies, as well as published draft assemblies of chimpanzee. We find that upwards of 40% of all gene families are inferred to have the wrong number of genes in draft assemblies, and that these incorrect assemblies both add and subtract genes. Using simulated genome assemblies of Drosophila melanogaster, we find that the major cause of increased gene numbers in draft genomes is the fragmentation of genes onto multiple individual contigs. Finally, we demonstrate the usefulness of RNA-Seq in improving the gene annotation of draft assemblies, largely by connecting genes that have been fragmented in the assembly process.

Beryllium fabrication/cost assessment for ITER (International Thermonuclear Experimental Reactor)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Beeston, J.M.; Longhurst, G.R.; Parsonage, T.

1990-06-01

A fabrication and cost estimate of three possible beryllium shapes for the International Thermonuclear Experimental Reactor (ITER) blanket is presented. The fabrication method by hot pressing (HP), cold isostatic pressing plus sintering (CIP+S), cold isostatic pressing plus sintering plus hot isostatic pressing (CIP+S+HIP), and sphere production by atomization or rotary electrode will be discussed. Conventional hot pressing blocks of beryllium with subsequent machining to finished shapes can be more expensive than production of a net shape by cold isostatic pressing and sintering. The three beryllium shapes to be considered here and proposed for ITER are: (1) cubic blocks (3 tomore » 17 cm on an edge), (2) tubular cylinders (33 to 50 mm i.d. by 62 mm o.d. by 8 m long), and (3) spheres (1--5 mm dia.). A rough cost estimate of the basic shape is presented which would need to be refined if the surface finish and tolerances required are better than the sintering process produces. The final cost of the beryllium in the blanket will depend largely on the machining and recycling of beryllium required to produce the finished product. The powder preparation will be discussed before shape fabrication. 10 refs., 6 figs.« less

Design and manufacture a coconut milk squeezer

NASA Astrophysics Data System (ADS)

Wayan Surata, I.; Gde Tirta Nindhia, Tjokorda; Budyanto, D.; Yulianto, A. E.

2017-05-01

The process of cooking oil production generally is started by grating the ripe coconut meat, then pressing the grated meat to obtain coconut milk, and finally heating the coconut milk to obtain the cooking oil. Pressing mechanism to obtain coconut milk is a very important step and decisive in the process of producing cooking oil. The amount of milk produced depends on the pressure applied at the time of pressing grated coconut. The higher the pressure, the more milk is obtained. Some commercial mechanical pressing tools that available in the market are not efficient due to the working steps too much and take long time per cycle of work. The aims of this study was to design and manufacture a power screw squeezer for the collection of coconut milk. Power screw produces a compressive force in the cylinder to push and press the grated coconut until the end of the cylinder while the coconut milk and coconut dregs flow out simultaneously. Screw press was designed using straight shaft configuration with square profile. Performance test was done to investigate the actual capacity and yield of milk produced. The results showed that squeezer of grated coconut worked well with capacity an average of 13,63 kg/h and coconut milk yield of 58%.

Israel: Background and U.S. Relations in Brief

DTIC Science & Technology

2017-02-24

further negotiation . The status of the Gaza Strip is a final status issue to be resolved through negotiations . Israel proclaimed Jerusalem as its...largely anticipated a negotiated conflict-ending outcome that would result in two states.8 In a White House press conference on February 15, 2017...pending negotiations between Israel, Egypt, Jordan, and Palestinian representatives on the territories’ final status. April 1987 – Secret London

Assembly line termination in cylindrocyclophane biosynthesis: discovery of an editing type II thioesterase domain in a type I polyketide synthase† †Electronic supplementary information (ESI) available: Fig. S1–S12; Tables S1–S8, full experimental details and procedures, 1H and 13C NMR spectral data and HRMS data of compounds 10 and 11 and the internal standards. See DOI: 10.1039/c4sc03132f Click here for additional data file.

PubMed Central

Nakamura, H.; Wang, J. X.

2015-01-01

The termination step is an important source of structural diversity in polyketide biosynthesis. Most type I polyketide synthase (PKS) assembly lines are terminated by a thioesterase (TE) domain located at the C-terminus of the final module, while other PKS assembly lines lack a terminal TE domain and are instead terminated by a separate enzyme in trans. In cylindrocyclophane biosynthesis, the type I modular PKS assembly line is terminated by a freestanding type III PKS (CylI). Unexpectedly, the final module of the type I PKS (CylH) also possesses a C-terminal TE domain. Unlike typical type I PKSs, the CylH TE domain does not influence assembly line termination by CylI in vitro. Instead, this domain phylogenetically resembles a type II TE and possesses activity consistent with an editing function. This finding may shed light on the evolution of unusual PKS termination logic. In addition, the presence of related type II TE domains in many cryptic type I PKS and nonribosomal peptide synthetase (NRPS) assembly lines has implications for pathway annotation, product prediction, and engineering. PMID:29218151

Quantifying cadherin mechanotransduction machinery assembly/disassembly dynamics using fluorescence covariance analysis.

PubMed

Vedula, Pavan; Cruz, Lissette A; Gutierrez, Natasha; Davis, Justin; Ayee, Brian; Abramczyk, Rachel; Rodriguez, Alexis J

2016-06-30

Quantifying multi-molecular complex assembly in specific cytoplasmic compartments is crucial to understand how cells use assembly/disassembly of these complexes to control function. Currently, biophysical methods like Fluorescence Resonance Energy Transfer and Fluorescence Correlation Spectroscopy provide quantitative measurements of direct protein-protein interactions, while traditional biochemical approaches such as sub-cellular fractionation and immunoprecipitation remain the main approaches used to study multi-protein complex assembly/disassembly dynamics. In this article, we validate and quantify multi-protein adherens junction complex assembly in situ using light microscopy and Fluorescence Covariance Analysis. Utilizing specific fluorescently-labeled protein pairs, we quantified various stages of adherens junction complex assembly, the multiprotein complex regulating epithelial tissue structure and function following de novo cell-cell contact. We demonstrate: minimal cadherin-catenin complex assembly in the perinuclear cytoplasm and subsequent localization to the cell-cell contact zone, assembly of adherens junction complexes, acto-myosin tension-mediated anchoring, and adherens junction maturation following de novo cell-cell contact. Finally applying Fluorescence Covariance Analysis in live cells expressing fluorescently tagged adherens junction complex proteins, we also quantified adherens junction complex assembly dynamics during epithelial monolayer formation.

Recent Advances in Multi-component Particles Assembly.

PubMed

Guo, Dan; Song, Yanlin

2018-03-09

Particles assembly and co-assembly have been research frontiers in chemistry and material science in the past few decades. To achieve a large variety of intricate structures and functional materials, remarkable progress has been made in the particle assembly principles and strategies. It can be summarized that the particle assembly is driven by intrinsic interparticle interaction or the external control. In this article, we focus on binary or ternary particles co-assembly and review the principles and feasible strategies. These advances have led to new disciplines of microfabrication technology and material engineering. Although remarked achievement on particle-based structures has been made, it is still challenging to fully develop general and facile strategies to precisely control the one-dimensional (1D) co-assembly. This article reviews the recent development on multi-component particles co-assembly, which significantly increases structural complexity and functional diversity. In particular, we highlight the advances in the particles co-assembly of well-ordered 1D binary superstructures by liquid soft confinement. Finally, prospective outlook for future trends in this field is proposed. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Advanced Computer Simulations of Military Incinerators

DTIC Science & Technology

2004-12-01

Reaction Engineering International (REI) has developed advanced computer simulation tools for analyzing chemical demilitarization incinerators. The...Manager, 2003a: Summary of Engineering Design Study Projectile Washout System (PWS) Testing. Assembled Chemical Weapons Alternatives (ACWA), Final... Engineering Design Studies for Demilitarization of Assembled Chemical Weapons at Pueblo Chemical Depot. O’Shea, L. et al, 2003: RIM 57 – Monitoring in

7 CFR 1755.501 - Definitions applicable to §§ 1755.501 through 1755.510.

Code of Federal Regulations, 2011 CFR

2011-01-01

... travel use built on or permanently attached to a self-propelled motor vehicle chassis or on a chassis cab... the FCC rules in 47 CFR part 68. NID. Network interface device. Primary station protector. An assembly... and determined that: (1) Final assembly or manufacture of the equipment is completed in the United...

76 FR 31354 - Notice of Issuance of Final Determination Concerning the Transit Connect Electric Vehicle

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-31

... base without a powertrain or exhaust components, and consists of a frame, body, axles, and wheels. The... substantially transformed in the U.S. and considered products of the U.S. The U.S. assembly occurs at various stations. The assembly stations at AM General, the manufacturing subcontractor, are described as follows...

Omokage search: shape similarity search service for biomolecular structures in both the PDB and EMDB.

PubMed

Suzuki, Hirofumi; Kawabata, Takeshi; Nakamura, Haruki

2016-02-15

Omokage search is a service to search the global shape similarity of biological macromolecules and their assemblies, in both the Protein Data Bank (PDB) and Electron Microscopy Data Bank (EMDB). The server compares global shapes of assemblies independent of sequence order and number of subunits. As a search query, the user inputs a structure ID (PDB ID or EMDB ID) or uploads an atomic model or 3D density map to the server. The search is performed usually within 1 min, using one-dimensional profiles (incremental distance rank profiles) to characterize the shapes. Using the gmfit (Gaussian mixture model fitting) program, the found structures are fitted onto the query structure and their superimposed structures are displayed on the Web browser. Our service provides new structural perspectives to life science researchers. Omokage search is freely accessible at http://pdbj.org/omokage/. © The Author 2015. Published by Oxford University Press.

Haemonchus contortus: Genome Structure, Organization and Comparative Genomics.

PubMed

Laing, R; Martinelli, A; Tracey, A; Holroyd, N; Gilleard, J S; Cotton, J A

2016-01-01

One of the first genome sequencing projects for a parasitic nematode was that for Haemonchus contortus. The open access data from the Wellcome Trust Sanger Institute provided a valuable early resource for the research community, particularly for the identification of specific genes and genetic markers. Later, a second sequencing project was initiated by the University of Melbourne, and the two draft genome sequences for H. contortus were published back-to-back in 2013. There is a pressing need for long-range genomic information for genetic mapping, population genetics and functional genomic studies, so we are continuing to improve the Wellcome Trust Sanger Institute assembly to provide a finished reference genome for H. contortus. This review describes this process, compares the H. contortus genome assemblies with draft genomes from other members of the strongylid group and discusses future directions for parasite genomics using the H. contortus model. Copyright © 2016 Elsevier Ltd. All rights reserved.

High-resolution Crystal Structure of Dimeric VP40 From Sudan ebolavirus.

PubMed

Clifton, Matthew C; Bruhn, Jessica F; Atkins, Kateri; Webb, Terry L; Baydo, Ruth O; Raymond, Amy; Lorimer, Donald D; Edwards, Thomas E; Myler, Peter J; Saphire, Erica Ollmann

2015-10-01

Ebolaviruses cause severe hemorrhagic fever. Central to the Ebola life cycle is the matrix protein VP40, which oligomerizes and drives viral budding. Here we present the crystal structure of the Sudan virus (SUDV) matrix protein. This structure is higher resolution (1.6 Å) than previously achievable. Despite differences in the protein purification, we find that it still forms a stable dimer in solution, as was noted for other Ebola VP40s. Although the N-terminal domain interface by which VP40 dimerizes is conserved between Ebola virus and SUDV, the C-terminal domain interface by which VP40 dimers may further assemble is significantly smaller in this SUDV assembly. © The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

STS_135_ MEDIA

NASA Image and Video Library

2011-06-30

JSC2011-E-060800 (30 June 2011) --- The STS-135 crew meets with the Johnson Space Center public affairs team before the crew's media briefing at NASA?s Johnson Space Center in Houston on June 30, 2011. The press conference provided the last scheduled opportunity for a large group of press to speak with the crew before the final launch on July 8. Shown, from left, are Rob Navias and James Hartsfield of public affairs, and NASA astronauts Sandy Magnus, Rex Walheim, Chris Ferguson and Doug Hurley. Photo credit: NASA Photo/Houston Chronicle, Smiley N. Pool

Mechanical behaviour of pressed and sintered titanium alloys obtained from prealloyed and blended elemental powders.

PubMed

Bolzoni, L; Esteban, P G; Ruiz-Navas, E M; Gordo, E

2012-10-01

The applicability of irregular prealloyed Ti-6Al-4V powder for the fabrication of titanium products by pressing and sintering and its employment as a master alloy to obtain the Ti-3Al-2.5V alloy was studied. To this end, the starting powders were characterised by dilatometry, differential thermal analysis and XRD. Green samples were obtained by cold uniaxial pressing, and the evolution of the microstructure over the sintering temperature range 900-1400°C was studied. The variation of the final density and mechanical properties with the sintering temperature was considered. Based on the study carried out, it can be stated that more reliable powders are needed to open the titanium market to new applications. A relative density of 95% and diverse microstructural features and mechanical properties equivalent to those of biomedical devices can be obtained by the pressing and sintering route. Copyright © 2012 Elsevier Ltd. All rights reserved.

The new design of final optics assembly on SG-III prototype facility

NASA Astrophysics Data System (ADS)

Li, Ping; Zhao, Runchang; Wang, Wei; Jia, Huaiting; Chen, Liangmin; Su, Jingqin

2014-09-01

To improve the performance of SG-III prototype facility (TIL-Technical Integration Line), final optics assembly (FOA) is re-designed. It contains that stray light and focusing ghosts are optimized, operational performance and environments are improved and the total thickness of optics is reduced. With the re-designed FOA, Some performance advantages are achieved. First, the optics damages are mitigated obviously, especially crystals and Focus lens; Second, stray light and focusing ghosts are controlled better that organic contamination sources inside FOA are eliminated; Third, maintenance and operation are more convenient for the atoms environment; Fourth, the focusable power on target is increased for lower B-integral.

Experimental analysis of the sheet metal forming behavior of newly developed press hardening steels

NASA Astrophysics Data System (ADS)

Meza-García, Enrique; Kräusel, Verena; Landgrebe, Dirk

2018-05-01

The aim of this work was the characterization of the newly developed press hardening sheet alloys 1800 PHS and 2000 PHS developed by SSAB with regard to their hot forming behavior on the basis of the experimental determination of relevant mechanical and technological properties. For this purpose conventional and non-conventional sheet metal testing methods were used. To determine the friction coefficient, the strip drawing test was applied, while the deep drawing cup test was used to determine the maximum draw depth. Finally, a V-bending test was carried out to evaluate the springback behavior of the investigated alloys by varying the blank temperature and quenching media. This work provides a technological guideline for the production of press hardened sheet parts made of these investigated sheet metals.

Upgrading short-read animal genome assemblies to chromosome level using comparative genomics and a universal probe set.

PubMed

Damas, Joana; O'Connor, Rebecca; Farré, Marta; Lenis, Vasileios Panagiotis E; Martell, Henry J; Mandawala, Anjali; Fowler, Katie; Joseph, Sunitha; Swain, Martin T; Griffin, Darren K; Larkin, Denis M

2017-05-01

Most recent initiatives to sequence and assemble new species' genomes de novo fail to achieve the ultimate endpoint to produce contigs, each representing one whole chromosome. Even the best-assembled genomes (using contemporary technologies) consist of subchromosomal-sized scaffolds. To circumvent this problem, we developed a novel approach that combines computational algorithms to merge scaffolds into chromosomal fragments, PCR-based scaffold verification, and physical mapping to chromosomes. Multigenome-alignment-guided probe selection led to the development of a set of universal avian BAC clones that permit rapid anchoring of multiple scaffolds to chromosomes on all avian genomes. As proof of principle, we assembled genomes of the pigeon ( Columbia livia ) and peregrine falcon ( Falco peregrinus ) to chromosome levels comparable, in continuity, to avian reference genomes. Both species are of interest for breeding, cultural, food, and/or environmental reasons. Pigeon has a typical avian karyotype (2n = 80), while falcon (2n = 50) is highly rearranged compared to the avian ancestor. By using chromosome breakpoint data, we established that avian interchromosomal breakpoints appear in the regions of low density of conserved noncoding elements (CNEs) and that the chromosomal fission sites are further limited to long CNE "deserts." This corresponds with fission being the rarest type of rearrangement in avian genome evolution. High-throughput multiple hybridization and rapid capture strategies using the current BAC set provide the basis for assembling numerous avian (and possibly other reptilian) species, while the overall strategy for scaffold assembly and mapping provides the basis for an approach that (provided metaphases can be generated) could be applied to any animal genome. © 2017 Damas et al.; Published by Cold Spring Harbor Laboratory Press.

Micro-Masonry: Construction of 3D Structures by Mesoscale Self-Assembly

PubMed Central

Fernandez, Javier G.; Khademhosseini, Ali

2010-01-01

A general method for construction of three dimensional structures by directed assembly of microscale polymeric sub-units is presented. Shape-controlled microgels are directed to assemble into different shapes by limiting their movement onto a molded substrate. The capillary forces, resulting from the presence of a liquid polymer, assemble the microgels in close contact with the rest of the units and with the free surface, the latter imposing the final geometry of the resulting construct. The result is a freestanding structure composed of one or multiple layers of sub-units assembled in a tightly packed conformation. The applicability of the technique for the construction of scaffolds with cell-laden sub-units is demonstrated. In addition, scaffolds formed by the sequential aggregation of sub-units are produced. PMID:20440697

DNAzyme-Based Logic Gate-Mediated DNA Self-Assembly.

PubMed

Zhang, Cheng; Yang, Jing; Jiang, Shuoxing; Liu, Yan; Yan, Hao

2016-01-13

Controlling DNA self-assembly processes using rationally designed logic gates is a major goal of DNA-based nanotechnology and programming. Such controls could facilitate the hierarchical engineering of complex nanopatterns responding to various molecular triggers or inputs. Here, we demonstrate the use of a series of DNAzyme-based logic gates to control DNA tile self-assembly onto a prescribed DNA origami frame. Logic systems such as "YES," "OR," "AND," and "logic switch" are implemented based on DNAzyme-mediated tile recognition with the DNA origami frame. DNAzyme is designed to play two roles: (1) as an intermediate messenger to motivate downstream reactions and (2) as a final trigger to report fluorescent signals, enabling information relay between the DNA origami-framed tile assembly and fluorescent signaling. The results of this study demonstrate the plausibility of DNAzyme-mediated hierarchical self-assembly and provide new tools for generating dynamic and responsive self-assembly systems.

Superconductive radiofrequency window assembly

DOEpatents

Phillips, Harry Lawrence; Elliott, Thomas S.

1998-01-01

The present invention is a superconducting radiofrequency window assembly for use in an electron beam accelerator. The srf window assembly (20) has a superconducting metal-ceramic design. The srf window assembly (20) comprises a superconducting frame (30), a ceramic plate (40) having a superconducting metallized area, and a superconducting eyelet (50) for sealing plate (40) into frame (30). The plate (40) is brazed to eyelet (50) which is then electron beam welded to frame (30). A method for providing a ceramic object mounted in a metal member to withstand cryogenic temperatures is also provided. The method involves a new metallization process for coating a selected area of a ceramic object with a thin film of a superconducting material. Finally, a method for assembling an electron beam accelerator cavity utilizing the srf window assembly is provided. The procedure is carried out within an ultra clean room to minimize exposure to particulates which adversely affect the performance of the cavity within the electron beam accelerator.

Superconductive radiofrequency window assembly

DOEpatents

Phillips, H.L.; Elliott, T.S.

1998-05-19

The present invention is a superconducting radiofrequency window assembly for use in an electron beam accelerator. The SRF window assembly has a superconducting metal-ceramic design. The SRF window assembly comprises a superconducting frame, a ceramic plate having a superconducting metallized area, and a superconducting eyelet for sealing plate into frame. The plate is brazed to eyelet which is then electron beam welded to frame. A method for providing a ceramic object mounted in a metal member to withstand cryogenic temperatures is also provided. The method involves a new metallization process for coating a selected area of a ceramic object with a thin film of a superconducting material. Finally, a method for assembling an electron beam accelerator cavity utilizing the SRF window assembly is provided. The procedure is carried out within an ultra clean room to minimize exposure to particulates which adversely affect the performance of the cavity within the electron beam accelerator. 11 figs.

Superconducting radiofrequency window assembly

DOEpatents

Phillips, Harry L.; Elliott, Thomas S.

1997-01-01

The present invention is a superconducting radiofrequency window assembly for use in an electron beam accelerator. The srf window assembly (20) has a superconducting metal-ceramic design. The srf window assembly (20) comprises a superconducting frame (30), a ceramic plate (40) having a superconducting metallized area, and a superconducting eyelet (50) for sealing plate (40) into frame (30). The plate (40) is brazed to eyelet (50) which is then electron beam welded to frame (30). A method for providing a ceramic object mounted in a metal member to withstand cryogenic temperatures is also provided. The method involves a new metallization process for coating a selected area of a ceramic object with a thin film of a superconducting material. Finally, a method for assembling an electron beam accelerator cavity utilizing the srf window assembly is provided. The procedure is carried out within an ultra clean room to minimize exposure to particulates which adversely affect the performance of the cavity within the electron beam accelerator.

Superconducting radiofrequency window assembly

DOEpatents

Phillips, H.L.; Elliott, T.S.

1997-03-11

The present invention is a superconducting radiofrequency window assembly for use in an electron beam accelerator. The srf window assembly has a superconducting metal-ceramic design. The srf window assembly comprises a superconducting frame, a ceramic plate having a superconducting metallized area, and a superconducting eyelet for sealing plate into frame. The plate is brazed to eyelet which is then electron beam welded to frame. A method for providing a ceramic object mounted in a metal member to withstand cryogenic temperatures is also provided. The method involves a new metallization process for coating a selected area of a ceramic object with a thin film of a superconducting material. Finally, a method for assembling an electron beam accelerator cavity utilizing the srf window assembly is provided. The procedure is carried out within an ultra clean room to minimize exposure to particulates which adversely affect the performance of the cavity within the electron beam accelerator. 11 figs.

Structure Assembly by a Heterogeneous Team of Robots Using State Estimation, Generalized Joints, and Mobile Parallel Manipulators

NASA Technical Reports Server (NTRS)

Komendera, Erik E.; Adhikari, Shaurav; Glassner, Samantha; Kishen, Ashwin; Quartaro, Amy

2017-01-01

Autonomous robotic assembly by mobile field robots has seen significant advances in recent decades, yet practicality remains elusive. Identified challenges include better use of state estimation to and reasoning with uncertainty, spreading out tasks to specialized robots, and implementing representative joining methods. This paper proposes replacing 1) self-correcting mechanical linkages with generalized joints for improved applicability, 2) assembly serial manipulators with parallel manipulators for higher precision and stability, and 3) all-in-one robots with a heterogeneous team of specialized robots for agent simplicity. This paper then describes a general assembly algorithm utilizing state estimation. Finally, these concepts are tested in the context of solar array assembly, requiring a team of robots to assemble, bond, and deploy a set of solar panel mockups to a backbone truss to an accuracy not built into the parts. This paper presents the results of these tests.

Lightweight Small Arms Technologies

DTIC Science & Technology

2006-11-01

conducted using several methods. Initial measurements were obtained using a strand burner , followed by closed bomb measurements using both pressed... pellets and entire cases. Specialized fixtures were developed to measure primer and booster combustion properties. The final verification of interior

Discrete State Change Model of Manufacturing Quality to Aid Assembly Process Design

NASA Astrophysics Data System (ADS)

Koga, Tsuyoshi; Aoyama, Kazuhiro

This paper proposes a representation model of the quality state change in an assembly process that can be used in a computer-aided process design system. In order to formalize the state change of the manufacturing quality in the assembly process, the functions, operations, and quality changes in the assembly process are represented as a network model that can simulate discrete events. This paper also develops a design method for the assembly process. The design method calculates the space of quality state change and outputs a better assembly process (better operations and better sequences) that can be used to obtain the intended quality state of the final product. A computational redesigning algorithm of the assembly process that considers the manufacturing quality is developed. The proposed method can be used to design an improved manufacturing process by simulating the quality state change. A prototype system for planning an assembly process is implemented and applied to the design of an auto-breaker assembly process. The result of the design example indicates that the proposed assembly process planning method outputs a better manufacturing scenario based on the simulation of the quality state change.

Assemblathon 2: evaluating de novo methods of genome assembly in three vertebrate species

PubMed Central

2013-01-01

Background The process of generating raw genome sequence data continues to become cheaper, faster, and more accurate. However, assembly of such data into high-quality, finished genome sequences remains challenging. Many genome assembly tools are available, but they differ greatly in terms of their performance (speed, scalability, hardware requirements, acceptance of newer read technologies) and in their final output (composition of assembled sequence). More importantly, it remains largely unclear how to best assess the quality of assembled genome sequences. The Assemblathon competitions are intended to assess current state-of-the-art methods in genome assembly. Results In Assemblathon 2, we provided a variety of sequence data to be assembled for three vertebrate species (a bird, a fish, and snake). This resulted in a total of 43 submitted assemblies from 21 participating teams. We evaluated these assemblies using a combination of optical map data, Fosmid sequences, and several statistical methods. From over 100 different metrics, we chose ten key measures by which to assess the overall quality of the assemblies. Conclusions Many current genome assemblers produced useful assemblies, containing a significant representation of their genes and overall genome structure. However, the high degree of variability between the entries suggests that there is still much room for improvement in the field of genome assembly and that approaches which work well in assembling the genome of one species may not necessarily work well for another. PMID:23870653

Automated solar panel assembly line

NASA Technical Reports Server (NTRS)

Somberg, H.

1981-01-01

The initial stage of the automated solar panel assembly line program was devoted to concept development and proof of approach through simple experimental verification. In this phase, laboratory bench models were built to demonstrate and verify concepts. Following this phase was machine design and integration of the various machine elements. The third phase was machine assembly and debugging. In this phase, the various elements were operated as a unit and modifications were made as required. The final stage of development was the demonstration of the equipment in a pilot production operation.

An improved NSGA - II algorithm for mixed model assembly line balancing

NASA Astrophysics Data System (ADS)

Wu, Yongming; Xu, Yanxia; Luo, Lifei; Zhang, Han; Zhao, Xudong

2018-05-01

Aiming at the problems of assembly line balancing and path optimization for material vehicles in mixed model manufacturing system, a multi-objective mixed model assembly line (MMAL), which is based on optimization objectives, influencing factors and constraints, is established. According to the specific situation, an improved NSGA-II algorithm based on ecological evolution strategy is designed. An environment self-detecting operator, which is used to detect whether the environment changes, is adopted in the algorithm. Finally, the effectiveness of proposed model and algorithm is verified by examples in a concrete mixing system.

NSI-1 Squib adapter development and final test report for usage on space shuttle gas sampler valve/bottle assembly 3270

NASA Technical Reports Server (NTRS)

Siebel, J. E.

1983-01-01

The possibility of utilizing the NSI-1 squib in place cartridge assembly 2270 for the function of both events required for the Space Shuttle Gas Sampler Valve/Bottle Assembly 3270 was determined. Additionally, it was a requirement that the closure disk of the NSI-1 squib and explosive residue therefrom be retained from the valve cavity in so far as possible to prevent any significant particulate from scratching the valve bore and causing sample leakage following the postfire 2 event.

Closeup view of the Solid Rocket Booster (SRB) Forward Skirt, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Close-up view of the Solid Rocket Booster (SRB) Forward Skirt, Frustum and Nose Cap mated assembly undergoing final preparations in the Solid Rocket Booster Assembly and Refurbishment Facility at Kennedy Space Center. The prominent feature in this view is the Forward Thrust Attach Fitting which mates up with the Forward Thrust Attach Fitting of the External Tank (ET) at the ends of the SRB Beam that runs through the ET's Inter Tank Assembly. - Space Transportation System, Solid Rocket Boosters, Lyndon B. Johnson Space Center, 2101 NASA Parkway, Houston, Harris County, TX

Structure of a designed protein cage that self-assembles into a highly porous cube

DOE PAGES

Lai, Yen-Ting; Reading, Eamonn; Hura, Greg L.; ...

2014-11-10

Natural proteins can be versatile building blocks for multimeric, self-assembling structures. Yet, creating protein-based assemblies with specific geometries and chemical properties remains challenging. Highly porous materials represent particularly interesting targets for designed assembly. Here we utilize a strategy of fusing two natural protein oligomers using a continuous alpha-helical linker to design a novel protein that self assembles into a 750 kDa, 225 Å diameter, cube-shaped cage with large openings into a 130 Å diameter inner cavity. A crystal structure of the cage showed atomic level agreement with the designed model, while electron microscopy, native mass spectrometry, and small angle x-raymore » scattering revealed alternate assembly forms in solution. These studies show that accurate design of large porous assemblies with specific shapes is feasible, while further specificity improvements will likely require limiting flexibility to select against alternative forms. Finally, these results provide a foundation for the design of advanced materials with applications in bionanotechnology, nanomedicine and material sciences.« less

Experimentally testing and assessing the predictive power of species assembly rules for tropical canopy ants

PubMed Central

Fayle, Tom M; Eggleton, Paul; Manica, Andrea; Yusah, Kalsum M; Foster, William A

2015-01-01

Understanding how species assemble into communities is a key goal in ecology. However, assembly rules are rarely tested experimentally, and their ability to shape real communities is poorly known. We surveyed a diverse community of epiphyte-dwelling ants and found that similar-sized species co-occurred less often than expected. Laboratory experiments demonstrated that invasion was discouraged by the presence of similarly sized resident species. The size difference for which invasion was less likely was the same as that for which wild species exhibited reduced co-occurrence. Finally we explored whether our experimentally derived assembly rules could simulate realistic communities. Communities simulated using size-based species assembly exhibited diversities closer to wild communities than those simulated using size-independent assembly, with results being sensitive to the combination of rules employed. Hence, species segregation in the wild can be driven by competitive species assembly, and this process is sufficient to generate observed species abundance distributions for tropical epiphyte-dwelling ants. PMID:25622647

Manual braille writer

DOEpatents

Hawk, L.S.; Turner, J.H.

1992-07-28

A manual-type braille writer is described that provides for both writing and reading in a normal left-to-right manner. In the preferred form, this braille writer has a clip board type base, and in the preferred embodiment a guide plate assembly can be moved to, and releasable fixed at, selected vertical locations along this base. The guide plate assembly is provided with a plurality of character cells uniformly spaced along rows across the guide plate assembly as well as in uniformly spaced rows. This guide plate assembly has a lower portion to be placed under a sheet of paper positioned on the clip board base and an upper portion to be positioned on top of the sheet. This upper portion is hinged with respect to the lower portion. Each character cell is typically made up of six appropriately spaced pins extending up from the lower portion that are aligned with a rosette-shaped cutout in the upper portion. A stylus member is provided that has a distal end to be fitted into the cutout of the character cell so that a recess in the end thereof presses the writing paper over the pin associated with that recess to produce a braille dot at that location. When desired, the upper portion can be lifted up so that the text already written can be read or to determine the place for initiating writing when writing has been interrupted. 10 figs.

Manual braille writer

DOEpatents

Hawk, Lawrence S.; Turner, Joe H.

1992-01-01

A manual-type braille writer that provides for both writing and reading in a normal left-to-right manner. In the preferred form, this braille writer has a clip board type base, and in the preferred embodiment a guide plate assembly can be moved to, and releasable fixed at, selected vertical locations along this base. The guide plate assembly is provided with a plurality of character cells uniformly spaced along rows across the guide plate assembly as well as in uniformly spaced rows. This guide plate assembly has a lower portion to be placed under a sheet of paper positioned on the clip board base and an upper portion to be positioned on top of the sheet. This upper portion is hinged with respect to the lower portion. Each character cell is typically made up of six appropriately spaced pins extending up from the lower portion that are aligned with a rosette-shaped cutout in the upper portion. A stylus member is provided that has a distal end to be fitted into the cutout of the character cell so that a recess in the end thereof presses the writing paper over the pin associated with that recess to produce a braille dot at that location. When desired, the upper portion can be lifted up so that the text already written can be read or to determine the place for initiating writing when writing has been interrupted.

A centrosomal protein FOR20 regulates microtubule assembly dynamics and plays a role in cell migration.

PubMed

Srivastava, Shalini; Panda, Dulal

2017-08-10

Here, we report that a centrosomal protein FOR20 [FOP (FGFR1 (fibroblast growth factor receptor 1) oncogene protein)-like protein of molecular mass of 20 kDa; also named as C16orf63, FLJ31153 or PHSECRG2] can regulate the assembly and stability of microtubules. Both FOR20 IgG antibody and GST (glutathione S -transferase)-tagged FOR20 could precipitate tubulin from the HeLa cell extract, indicating a possible interaction between FOR20 and tubulin. FOR20 was also detected in goat brain tissue extract and it cycled with microtubule-associated proteins. Furthermore, FOR20 bound to purified tubulin and inhibited the assembly of tubulin in vitro. The overexpression of FOR20 depolymerized interphase microtubules and the depletion of FOR20 prevented nocodazole-induced depolymerization of microtubules in HeLa cells. In addition, the depletion of FOR20 suppressed the dynamics of individual microtubules in live HeLa cells. FOR20-depleted MDA-MB-231 cells displayed zigzag motion and migrated at a slower rate than the control cells, indicating that FOR20 plays a role in directed cell migration. The results suggested that the centrosomal protein FOR20 is a new member of the microtubule-associated protein family and that it regulates the assembly and dynamics of microtubules. © 2017 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

Deletion of L4 domains reveals insights into the importance of ribosomal protein extensions in eukaryotic ribosome assembly.

PubMed

Gamalinda, Michael; Woolford, John L

2014-11-01

Numerous ribosomal proteins have a striking bipartite architecture: a globular body positioned on the ribosomal exterior and an internal loop buried deep into the rRNA core. In eukaryotes, a significant number of conserved r-proteins have evolved extra amino- or carboxy-terminal tail sequences, which thread across the solvent-exposed surface. The biological importance of these extended domains remains to be established. In this study, we have investigated the universally conserved internal loop and the eukaryote-specific extensions of yeast L4. We show that in contrast to findings with bacterial L4, deleting the internal loop of yeast L4 causes severely impaired growth and reduced levels of large ribosomal subunits. We further report that while depleting the entire L4 protein blocks early assembly steps in yeast, deletion of only its extended internal loop affects later steps in assembly, revealing a second role for L4 during ribosome biogenesis. Surprisingly, deletion of the entire eukaryote-specific carboxy-terminal tail of L4 has no effect on viability, production of 60S subunits, or translation. These unexpected observations provide impetus to further investigate the functions of ribosomal protein extensions, especially eukaryote-specific examples, in ribosome assembly and function. © 2014 Gamalinda and Woolford; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

Single-molecule sequencing and optical mapping yields an improved genome of woodland strawberry (Fragaria vesca) with chromosome-scale contiguity.

PubMed

Edger, Patrick P; VanBuren, Robert; Colle, Marivi; Poorten, Thomas J; Wai, Ching Man; Niederhuth, Chad E; Alger, Elizabeth I; Ou, Shujun; Acharya, Charlotte B; Wang, Jie; Callow, Pete; McKain, Michael R; Shi, Jinghua; Collier, Chad; Xiong, Zhiyong; Mower, Jeffrey P; Slovin, Janet P; Hytönen, Timo; Jiang, Ning; Childs, Kevin L; Knapp, Steven J

2018-02-01

Although draft genomes are available for most agronomically important plant species, the majority are incomplete, highly fragmented, and often riddled with assembly and scaffolding errors. These assembly issues hinder advances in tool development for functional genomics and systems biology. Here we utilized a robust, cost-effective approach to produce high-quality reference genomes. We report a near-complete genome of diploid woodland strawberry (Fragaria vesca) using single-molecule real-time sequencing from Pacific Biosciences (PacBio). This assembly has a contig N50 length of ∼7.9 million base pairs (Mb), representing a ∼300-fold improvement of the previous version. The vast majority (>99.8%) of the assembly was anchored to 7 pseudomolecules using 2 sets of optical maps from Bionano Genomics. We obtained ∼24.96 Mb of sequence not present in the previous version of the F. vesca genome and produced an improved annotation that includes 1496 new genes. Comparative syntenic analyses uncovered numerous, large-scale scaffolding errors present in each chromosome in the previously published version of the F. vesca genome. Our results highlight the need to improve existing short-read based reference genomes. Furthermore, we demonstrate how genome quality impacts commonly used analyses for addressing both fundamental and applied biological questions. © The Authors 2017. Published by Oxford University Press.

Novel transcriptome resources for three scleractinian coral species from the Indo-Pacific.

PubMed

Kenkel, Carly D; Bay, Line K

2017-09-01

Transcriptomic resources for coral species can provide insight into coral evolutionary history and stress-response physiology. Goniopora columna, Galaxea astreata, and Galaxea acrhelia are scleractinian corals of the Indo-Pacific, representing a diversity of morphologies and life-history traits. G. columna and G. astreata are common and cosmopolitan, while G. acrhelia is largely restricted to the coral triangle and Great Barrier Reef. Reference transcriptomes for these species were assembled from replicate colony fragments exposed to elevated (31°C) and ambient (27°C) temperatures. Trinity was used to create de novo assemblies for each species from 92-102 million raw Illumina Hiseq 2 × 150 bp reads. Host-specific assemblies contained 65 460-72 405 contigs, representing 26 693-37 894 isogroups (∼genes) with an average N50 of 2254. Gene name and/or gene ontology annotations were possible for 58% of isogroups on average. Transcriptomes contained 93.1-94.3% of EuKaryotic Orthologous Groups comprising the core eukaryotic gene set, and 89.98-91.92% of the single-copy metazoan core gene set orthologs were complete, indicating fairly comprehensive assemblies. This work expands the complement of transcriptomic resources available for scleractinian coral species, including the first reference for a representative of Goniopora spp. as well as species with novel morphology. © The Authors 2017. Published by Oxford University Press.

Axial displacements in external and internal implant-abutment connection.

PubMed

Lee, Ji-Hye; Kim, Dae-Gon; Park, Chan-Jin; Cho, Lee-Ra

2014-02-01

The purpose of this study was to evaluate the axial displacement of the abutments during clinical procedures by the tightening torque and cyclic loading. Two different implant-abutment connection systems were used (external butt joint connection [EXT]; internal tapered conical connection [INT]). The master casts with two implant replicas, angulated 10° from each other, were fabricated for each implant connection system. Four types of impression copings were assembled and tightened with the corresponding implants (hex transfer impression coping, non-hex transfer impression coping, hex pick-up impression coping, non-hex pick-up impression coping). Resin splinted abutments and final prosthesis were assembled. The axial displacement was measured from the length of each assembly, which was evaluated repeatedly, after 30 Ncm torque tightening. After 250 N cyclic loading of final prosthesis for 1,000,000 cycles, additional axial displacement was recorded. The mean axial displacement was statistically analyzed (repeated measured ANOVA). There was more axial displacement in the INT group than that of the EXT group in impression copings, resin splinted abutments, and final prosthesis. Less axial displacement was found at 1-piece non-hex transfer type impression coping than other type of impression copings in the INT group. There was more axial displacement at the final prosthesis than resin splinted abutments in the INT and the EXT groups. After 250 N cyclic loading of final prosthesis, the INT group showed more axial displacement than that of the EXT group. Internal tapered conical connection demonstrated a varying amount of axial displacement with tightening torque and cyclic loading. © 2012 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd.

Final Environmental Assessment for Buckley Air Force Base Military Construction

DTIC Science & Technology

2003-05-06

Colorado State University , Ft. Collins, CO, July 2000. COANG, 2000d. Final Air Emissions Inventory, 140th Fighter Wing, Buckley Air National Guard Base...Denver International Airport, Denver CO, March 1998. Guennel, 1995. Guennel, GK. Guide to Colorado Wildflowers, Westcliffe Publishers, Inc, Englewood...CO, 1995. Hoogland, 1995. Hoogland, John L. The Black-Tailed Prairie Dog- Social Life of a Burrowing Mammal. The University of Chicago Press

Advanced membrane electrode assemblies for fuel cells

DOEpatents

Kim, Yu Seung; Pivovar, Bryan S.

2012-07-24

A method of preparing advanced membrane electrode assemblies (MEA) for use in fuel cells. A base polymer is selected for a base membrane. An electrode composition is selected to optimize properties exhibited by the membrane electrode assembly based on the selection of the base polymer. A property-tuning coating layer composition is selected based on compatibility with the base polymer and the electrode composition. A solvent is selected based on the interaction of the solvent with the base polymer and the property-tuning coating layer composition. The MEA is assembled by preparing the base membrane and then applying the property-tuning coating layer to form a composite membrane. Finally, a catalyst is applied to the composite membrane.

Evaluation of beryllium for space shuttle components

NASA Technical Reports Server (NTRS)

Trapp, A. E.

1972-01-01

Application of beryllium to specific full-scale space shuttle structural components and assemblies was studied. Material evaluations were conducted to check the mechanical properties of as-received material to gain design information on characteristics needed for the material in the space shuttle environment, and to obtain data needed for evaluating component and panel tests. Four beryllium structural assemblies were analyzed and designed. Selected components of these assemblies, representing areas of critical loading or design/process uncertainty, were designed and tested, and two panel assemblies were fabricated. Trends in cost and weight factors were determined by progressive estimation at key points of preliminary design, final design, and fabrication to aid in a cost/weight evaluation of the use of beryllium.

DNA nanotechnology

NASA Astrophysics Data System (ADS)

Seeman, Nadrian C.; Sleiman, Hanadi F.

2018-01-01

DNA is the molecule that stores and transmits genetic information in biological systems. The field of DNA nanotechnology takes this molecule out of its biological context and uses its information to assemble structural motifs and then to connect them together. This field has had a remarkable impact on nanoscience and nanotechnology, and has been revolutionary in our ability to control molecular self-assembly. In this Review, we summarize the approaches used to assemble DNA nanostructures and examine their emerging applications in areas such as biophysics, diagnostics, nanoparticle and protein assembly, biomolecule structure determination, drug delivery and synthetic biology. The introduction of orthogonal interactions into DNA nanostructures is discussed, and finally, a perspective on the future directions of this field is presented.

Advanced membrane electrode assemblies for fuel cells

DOEpatents

Kim, Yu Seung; Pivovar, Bryan S

2014-02-25

A method of preparing advanced membrane electrode assemblies (MEA) for use in fuel cells. A base polymer is selected for a base membrane. An electrode composition is selected to optimize properties exhibited by the membrane electrode assembly based on the selection of the base polymer. A property-tuning coating layer composition is selected based on compatibility with the base polymer and the electrode composition. A solvent is selected based on the interaction of the solvent with the base polymer and the property-tuning coating layer composition. The MEA is assembled by preparing the base membrane and then applying the property-tuning coating layer to form a composite membrane. Finally, a catalyst is applied to the composite membrane.

KSC-03pd0947

NASA Image and Video Library

2003-04-02

KENNEDY SPACE CENTER, FLA. - A female osprey occupies a nest recently constructed on a speaker in the lower parking lot of the Press Site. Eggs have been sighted in the nest. The NASA logo seen in the background is on the nearby Vehicle Assembly Building. Known as a fish hawk, the osprey selects sites of opportunity, from trees and telephone poles to rocks or even flat ground. In the United States it is found from Alaska to Florida and the Gulf Coast. Osprey nests are found throughout the Kennedy Space Center and surrounding Merritt Island National Wildlife Refuge.

Conceptual Design of a Basic Production Facility for the XM587E2/XM724 Electronic Time Fuzes

DTIC Science & Technology

1977-11-01

blue side up, and then staked. The spri.ng pin is pressed in position and probed for the 1. 644-0. 010- inch dimension. See figure 33. 4.6.7.2 Parts...fitting subassembly. The detonator 69 IDLE HOPPER FEED -PROBE STAKE SPRING PIN PROBE PRESENCE STAKE LEADL IPROB LEADID ~ ASSEMBLY14 3 1 BLUE SIDE UP...automatic shutoffs. * Warning lights /alarms/ signs /’Jecals where necessary. * Electrical grounding of machine. [ 98 0 Noise levels below 85 decibals at

KSC-97pc231

NASA Image and Video Library

1997-01-29

The filming of scenes for the movie "Contact" by Warner Bros.' cast and crew at Kennedy Space Center's Launch Complex 39 Press Site on January 29 is captured by cameras on the roof of the Vehicle Assembly Building. The screenplay for "Contact" is based on the best-selling novel by the late astronomer Carl Sagan. The cast includes Jodie Foster, Matthew McConaughey, John Hurt, James Woods, Tom Skerritt, David Morse, William Fichtner, Rob Lowe and Angela Bassett. Described by Warner Bros. as a science fiction drama, "Contact" will depict humankind's first encounter with evidence of extraterrestrial life

KSC-97pc230

NASA Image and Video Library

1997-01-29

The filming of scenes for the movie "Contact" by Warner Bros.' cast and crew at Kennedy Space Center's Launch Complex 39 Press Site on January 29 is captured by cameras on the roof of the Vehicle Assembly Building. The screenplay for "Contact" is based on the best-selling novel by the late astronomer Carl Sagan. The cast includes Jodie Foster, Matthew McConaughey, John Hurt, James Woods, Tom Skerritt, David Morse, William Fichtner, Rob Lowe and Angela Bassett. Described by Warner Bros. as a science fiction drama, "Contact" will depict humankind's first encounter with evidence of extraterrestrial life

KSC-97pc228

NASA Image and Video Library

1997-01-29

With the Vehicle Assembly Building looming in the background, Warner Bros.' cast and crew are filming scenes for the movie "Contact" at Kennedy Space Center's Launch Complex 39 Press Site on January 29. The screenplay for "Contact" is based on the best-selling novel by the late astronomer Carl Sagan. The cast includes Jodie Foster, Matthew McConaughey, John Hurt, James Woods, Tom Skerritt, David Morse, William Fichtner, Rob Lowe and Angela Bassett. Described by Warner Bros. as a science fiction drama, "Contact" will depict humankind's first encounter with evidence of extraterrestrial life

KSC-97pc229

NASA Image and Video Library

1997-01-29

The filming of scenes for the movie "Contact" by Warner Bros.' cast and crew at Kennedy Space Center's Launch Complex 39 Press Site on January 29 is captured by cameras on the roof of the Vehicle Assembly Building. The screenplay for "Contact" is based on the best-selling novel by the late astronomer Carl Sagan. The cast includes Jodie Foster, Matthew McConaughey, John Hurt, James Woods, Tom Skerritt, David Morse, William Fichtner, Rob Lowe and Angela Bassett. Described by Warner Bros. as a science fiction drama, "Contact" will depict humankind's first encounter with evidence of extraterrestrial life

KSC-97pc233

NASA Image and Video Library

1997-01-30

With the Vehicle Assembly Building looming in the background, Warner Bros.' cast and crew are filming scenes for the movie "Contact" at Kennedy Space Center's Launch Complex 39 Press Site on January 30. The screenplay for "Contact" is based on the best-selling novel by the late astronomer Carl Sagan. The cast includes Jodie Foster, Matthew McConaughey, John Hurt, James Woods, Tom Skerritt, David Morse, William Fichtner, Rob Lowe and Angela Bassett. Described by Warner Bros. as a science fiction drama, "Contact" will depict humankind's first encounter with evidence of extraterrestrial life

KSC-97pc225

NASA Image and Video Library

1997-01-29

With the Vehicle Assembly Building looming in the background, Warner Bros.' cast and crew are filming scenes for the movie "Contact" at Kennedy Space Center's Launch Complex 39 Press Site on January 29. The screenplay for "Contact" is based on the best-selling novel by the late astronomer Carl Sagan. The cast includes Jodie Foster, Matthew McConaughey, John Hurt, James Woods, Tom Skerritt, David Morse, William Fichtner, Rob Lowe and Angela Bassett. Described by Warner Bros. as a science fiction drama, "Contact" will depict humankind's first encounter with evidence of extraterrestrial life

The effects of motor vehicle window tinting on traffic safety and enforcement : final report : a report to the Governor and General Assembly in response to Senate Joint Resolution 293, 1993 Session.

DOT National Transportation Integrated Search

1994-01-01

The 1993 Session of the Virginia General Assembly lessened restrictions relating to the application of aftermarket tinted window films to motor vehicle glass. Effective July 1, 1993, vehicles are allowed to have window tinting treatments that do not ...

Comparison in partition efficiency of protein separation between four different tubing modifications in spiral high-speed countercurrent chromatography

PubMed Central

Ito, Yoichiro; Clary, Robert

2016-01-01

High-speed countercurrent chromatography with a spiral tube assembly can retain a satisfactory amount of stationary phase of polymer phase systems used for protein separation. In order to improve the partition efficiency a simple tool to modify the tubing shapes was fabricated, and the following four different tubing modifications were made: intermittently pressed at 10 mm width, flat, flat-wave, and flat-twist. Partition efficiencies of the separation column made from these modified tubing were examined in protein separation with an aqueous-aqueous polymer phase system at flow rates of 1–2 ml/min under 800 rpm. The results indicated that the column with all modified tubing improved the partition efficiency at a flow rate of 1 ml/min, but at a higher flow rate of 2 ml/min the columns made of flattened tubing showed lowered partition efficiency apparently due to the loss of the retained stationary phase. Among all the modified columns, the column with intermittently pressed tubing gave the best peak resolution. It may be concluded that the intermittently pressed and flat-twist improve the partition efficiency in a semi-preparative separation while other modified tubing of flat and flat-wave configurations may be used for analytical separations with a low flow rate. PMID:27790621

Comparison in partition efficiency of protein separation between four different tubing modifications in spiral high-speed countercurrent chromatography.

PubMed

Ito, Yoichiro; Clary, Robert

2016-12-01

High-speed countercurrent chromatography with a spiral tube assembly can retain a satisfactory amount of stationary phase of polymer phase systems used for protein separation. In order to improve the partition efficiency a simple tool to modify the tubing shapes was fabricated, and the following four different tubing modifications were made: intermittently pressed at 10 mm width, flat, flat-wave, and flat-twist. Partition efficiencies of the separation column made from these modified tubing were examined in protein separation with an aqueous-aqueous polymer phase system at flow rates of 1-2 ml/min under 800 rpm. The results indicated that the column with all modified tubing improved the partition efficiency at a flow rate of 1 ml/min, but at a higher flow rate of 2 ml/min the columns made of flattened tubing showed lowered partition efficiency apparently due to the loss of the retained stationary phase. Among all the modified columns, the column with intermittently pressed tubing gave the best peak resolution. It may be concluded that the intermittently pressed and flat-twist improve the partition efficiency in a semi-preparative separation while other modified tubing of flat and flat-wave configurations may be used for analytical separations with a low flow rate.

Rice SNP-seek database update: new SNPs, indels, and queries.

PubMed

Mansueto, Locedie; Fuentes, Roven Rommel; Borja, Frances Nikki; Detras, Jeffery; Abriol-Santos, Juan Miguel; Chebotarov, Dmytro; Sanciangco, Millicent; Palis, Kevin; Copetti, Dario; Poliakov, Alexandre; Dubchak, Inna; Solovyev, Victor; Wing, Rod A; Hamilton, Ruaraidh Sackville; Mauleon, Ramil; McNally, Kenneth L; Alexandrov, Nickolai

2017-01-04

We describe updates to the Rice SNP-Seek Database since its first release. We ran a new SNP-calling pipeline followed by filtering that resulted in complete, base, filtered and core SNP datasets. Besides the Nipponbare reference genome, the pipeline was run on genome assemblies of IR 64, 93-11, DJ 123 and Kasalath. New genotype query and display features are added for reference assemblies, SNP datasets and indels. JBrowse now displays BAM, VCF and other annotation tracks, the additional genome assemblies and an embedded VISTA genome comparison viewer. Middleware is redesigned for improved performance by using a hybrid of HDF5 and RDMS for genotype storage. Query modules for genotypes, varieties and genes are improved to handle various constraints. An integrated list manager allows the user to pass query parameters for further analysis. The SNP Annotator adds traits, ontology terms, effects and interactions to markers in a list. Web-service calls were implemented to access most data. These features enable seamless querying of SNP-Seek across various biological entities, a step toward semi-automated gene-trait association discovery. URL: http://snp-seek.irri.org. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Northern Spotted Owl (Strix occidentalis caurina) Genome: Divergence with the Barred Owl (Strix varia) and Characterization of Light-Associated Genes.

PubMed

Hanna, Zachary R; Henderson, James B; Wall, Jeffrey D; Emerling, Christopher A; Fuchs, Jérôme; Runckel, Charles; Mindell, David P; Bowie, Rauri C K; DeRisi, Joseph L; Dumbacher, John P

2017-10-01

We report here the assembly of a northern spotted owl (Strix occidentalis caurina) genome. We generated Illumina paired-end sequence data at 90× coverage using nine libraries with insert lengths ranging from ∼250 to 9,600 nt and read lengths from 100 to 375 nt. The genome assembly is comprised of 8,108 scaffolds totaling 1.26 × 109 nt in length with an N50 length of 3.98 × 106 nt. We calculated the genome-wide fixation index (FST) of S. o. caurina with the closely related barred owl (Strix varia) as 0.819. We examined 19 genes that encode proteins with light-dependent functions in our genome assembly as well as in that of the barn owl (Tyto alba). We present genomic evidence for loss of three of these in S. o. caurina and four in T. alba. We suggest that most light-associated gene functions have been maintained in owls and their loss has not proceeded to the same extent as in other dim-light-adapted vertebrates. © The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

Improved de novo genomic assembly for the domestic donkey.

PubMed

Renaud, Gabriel; Petersen, Bent; Seguin-Orlando, Andaine; Bertelsen, Mads Frost; Waller, Andrew; Newton, Richard; Paillot, Romain; Bryant, Neil; Vaudin, Mark; Librado, Pablo; Orlando, Ludovic

2018-04-01

Donkeys and horses share a common ancestor dating back to about 4 million years ago. Although a high-quality genome assembly at the chromosomal level is available for the horse, current assemblies available for the donkey are limited to moderately sized scaffolds. The absence of a better-quality assembly for the donkey has hampered studies involving the characterization of patterns of genetic variation at the genome-wide scale. These range from the application of genomic tools to selective breeding and conservation to the more fundamental characterization of the genomic loci underlying speciation and domestication. We present a new high-quality donkey genome assembly obtained using the Chicago HiRise assembly technology, providing scaffolds of subchromosomal size. We make use of this new assembly to obtain more accurate measures of heterozygosity for equine species other than the horse, both genome-wide and locally, and to detect runs of homozygosity potentially pertaining to positive selection in domestic donkeys. Finally, this new assembly allowed us to identify fine-scale chromosomal rearrangements between the horse and the donkey that likely played an active role in their divergence and, ultimately, speciation.

Improved de novo genomic assembly for the domestic donkey

PubMed Central

Newton, Richard; Paillot, Romain; Bryant, Neil; Vaudin, Mark

2018-01-01

Donkeys and horses share a common ancestor dating back to about 4 million years ago. Although a high-quality genome assembly at the chromosomal level is available for the horse, current assemblies available for the donkey are limited to moderately sized scaffolds. The absence of a better-quality assembly for the donkey has hampered studies involving the characterization of patterns of genetic variation at the genome-wide scale. These range from the application of genomic tools to selective breeding and conservation to the more fundamental characterization of the genomic loci underlying speciation and domestication. We present a new high-quality donkey genome assembly obtained using the Chicago HiRise assembly technology, providing scaffolds of subchromosomal size. We make use of this new assembly to obtain more accurate measures of heterozygosity for equine species other than the horse, both genome-wide and locally, and to detect runs of homozygosity potentially pertaining to positive selection in domestic donkeys. Finally, this new assembly allowed us to identify fine-scale chromosomal rearrangements between the horse and the donkey that likely played an active role in their divergence and, ultimately, speciation. PMID:29740610

Final Days

MedlinePlus

... Ferrell BR, Coyle N. Oxford Textbook of Palliative Nursing (3 rd ed. ). Oxford University Press New York: New York; 2010. Karnes B. Gone From My Sight. Vancouver, WA: BK Books; 2009. Approved by the HPNA Education Committee August 2008. Reviewed April 2012 orig. 08 ...

Press Oil Final Release Survey

DOE Office of Scientific and Technical Information (OSTI.GOV)

Whicker, Jeffrey Jay; Ruedig, Elizabeth

There are forty-eight 55 gallon barrels filled with hydraulic oil that are candidates for release and recycle. This oil needs to be characterized prior to release. Principles of sampling as provided in MARSAME/MARSSIM approaches were used as guidance for sampling.

Chaperoning 5S RNA assembly.

PubMed

Madru, Clément; Lebaron, Simon; Blaud, Magali; Delbos, Lila; Pipoli, Juliana; Pasmant, Eric; Réty, Stéphane; Leulliot, Nicolas

2015-07-01

In eukaryotes, three of the four ribosomal RNAs (rRNAs)—the 5.8S, 18S, and 25S/28S rRNAs—are processed from a single pre-rRNA transcript and assembled into ribosomes. The fourth rRNA, the 5S rRNA, is transcribed by RNA polymerase III and is assembled into the 5S ribonucleoprotein particle (RNP), containing ribosomal proteins Rpl5/uL18 and Rpl11/uL5, prior to its incorporation into preribosomes. In mammals, the 5S RNP is also a central regulator of the homeostasis of the tumor suppressor p53. The nucleolar localization of the 5S RNP and its assembly into preribosomes are performed by a specialized complex composed of Rpf2 and Rrs1 in yeast or Bxdc1 and hRrs1 in humans. Here we report the structural and functional characterization of the Rpf2-Rrs1 complex alone, in complex with the 5S RNA, and within pre-60S ribosomes. We show that the Rpf2-Rrs1 complex contains a specialized 5S RNA E-loop-binding module, contacts the Rpl5 protein, and also contacts the ribosome assembly factor Rsa4 and the 25S RNA. We propose that the Rpf2-Rrs1 complex establishes a network of interactions that guide the incorporation of the 5S RNP in preribosomes in the initial conformation prior to its rotation to form the central protuberance found in the mature large ribosomal subunit. © 2015 Madru et al.; Published by Cold Spring Harbor Laboratory Press.

Formation of RNA Granule-Derived Capsid Assembly Intermediates Appears To Be Conserved between Human Immunodeficiency Virus Type 1 and the Nonprimate Lentivirus Feline Immunodeficiency Virus.

PubMed

Reed, Jonathan C; Westergreen, Nick; Barajas, Brook C; Ressler, Dylan T B; Phuong, Daryl J; Swain, John V; Lingappa, Vishwanath R; Lingappa, Jaisri R

2018-05-01

During immature capsid assembly in cells, human immunodeficiency virus type 1 (HIV-1) Gag co-opts a host RNA granule, forming a pathway of intracellular assembly intermediates containing host components, including two cellular facilitators of assembly, ABCE1 and DDX6. A similar assembly pathway has been observed for other primate lentiviruses. Here we asked whether feline immunodeficiency virus (FIV), a nonprimate lentivirus, also forms RNA granule-derived capsid assembly intermediates. First, we showed that the released FIV immature capsid and a large FIV Gag-containing intracellular complex are unstable during analysis, unlike for HIV-1. We identified harvest conditions, including in situ cross-linking, that overcame this problem, revealing a series of FIV Gag-containing complexes corresponding in size to HIV-1 assembly intermediates. Previously, we showed that assembly-defective HIV-1 Gag mutants are arrested at specific assembly intermediates; here we identified four assembly-defective FIV Gag mutants, including three not previously studied, and demonstrated that they appear to be arrested at the same intermediate as the cognate HIV-1 mutants. Further evidence that these FIV Gag-containing complexes correspond to assembly intermediates came from coimmunoprecipitations demonstrating that endogenous ABCE1 and the RNA granule protein DDX6 are associated with FIV Gag, as shown previously for HIV-1 Gag, but are not associated with a ribosomal protein, at steady state. Additionally, we showed that FIV Gag associates with another RNA granule protein, DCP2. Finally, we validated the FIV Gag-ABCE1 and FIV Gag-DCP2 interactions with proximity ligation assays demonstrating colocalization in situ Together, these data support a model in which primate and nonprimate lentiviruses form intracellular capsid assembly intermediates derived from nontranslating host RNA granules. IMPORTANCE Like HIV-1 Gag, FIV Gag assembles into immature capsids; however, it is not known whether FIV Gag progresses through a pathway of immature capsid assembly intermediates derived from host RNA granules, as shown for HIV-1 Gag. Here we showed that FIV Gag forms complexes that resemble HIV-1 capsid assembly intermediates in size and in their association with ABCE1 and DDX6, two host facilitators of HIV-1 immature capsid assembly that are found in HIV-1 assembly intermediates. Our studies also showed that known and novel assembly-defective FIV Gag mutants fail to progress past putative intermediates in a pattern resembling that observed for HIV-1 Gag mutants. Finally, we used imaging to demonstrate colocalization of FIV Gag with ABCE1 and with the RNA granule protein DCP2. Thus, we conclude that formation of assembly intermediates derived from host RNA granules is likely conserved between primate and nonprimate lentiviruses and could provide targets for future antiviral strategies. Copyright © 2018 American Society for Microbiology.

The effects of combined elastic- and free-weight tension vs. free-weight tension on one-repetition maximum strength in the bench press.

PubMed

Bellar, David M; Muller, Matthew D; Barkley, Jacob E; Kim, Chul-Ho; Ida, Keisuke; Ryan, Edward J; Bliss, Mathew V; Glickman, Ellen L

2011-02-01

The present study investigated the effects of training combining elastic tension, free weights, and the bench press. Eleven college-aged men (untrained) in the bench press participated in the 13-week study. The participants were first given instructions and then practiced the bench press, followed by a one-repetition maximum (1RM) test of baseline strength. Subjects were then trained in the bench press for 3 weeks to allow for the beginning of neural adaptation. After another 1RM test, participants were assigned to 1 of 2 conditions for the next 3 weeks of training: 85% Free-Weight Tension, 15% Elastic Tension (BAND), or 100% Free-Weight Tension (STAND). After 3 weeks of training and a third 1RM max test, participants switched treatments, under which they completed the final 3 weeks of training and the fourth 1RM test. Analysis via analysis of covariance revealed a significant (p ≤ 0.05) main effect for time and interaction effect for Treatment (BAND vs. STAND). Subsequent analysis via paired-samples t-test revealed the BAND condition was significantly better (p = 0.05) at producing raw gains in 1RM strength. (BAND 9.95 ± 3.7 kg vs. STAND 7.56 ± 2.8 kg). These results suggest that the addition of elastic tension to the bench press may be an effective method of increasing strength.

A High-Resolution SNP Array-Based Linkage Map Anchors a New Domestic Cat Draft Genome Assembly and Provides Detailed Patterns of Recombination.

PubMed

Li, Gang; Hillier, LaDeana W; Grahn, Robert A; Zimin, Aleksey V; David, Victor A; Menotti-Raymond, Marilyn; Middleton, Rondo; Hannah, Steven; Hendrickson, Sher; Makunin, Alex; O'Brien, Stephen J; Minx, Pat; Wilson, Richard K; Lyons, Leslie A; Warren, Wesley C; Murphy, William J

2016-06-01

High-resolution genetic and physical maps are invaluable tools for building accurate genome assemblies, and interpreting results of genome-wide association studies (GWAS). Previous genetic and physical maps anchored good quality draft assemblies of the domestic cat genome, enabling the discovery of numerous genes underlying hereditary disease and phenotypes of interest to the biomedical science and breeding communities. However, these maps lacked sufficient marker density to order thousands of shorter scaffolds in earlier assemblies, which instead relied heavily on comparative mapping with related species. A high-resolution map would aid in validating and ordering chromosome scaffolds from existing and new genome assemblies. Here, we describe a high-resolution genetic linkage map of the domestic cat genome based on genotyping 453 domestic cats from several multi-generational pedigrees on the Illumina 63K SNP array. The final maps include 58,055 SNP markers placed relative to 6637 markers with unique positions, distributed across all autosomes and the X chromosome. Our final sex-averaged maps span a total autosomal length of 4464 cM, the longest described linkage map for any mammal, confirming length estimates from a previous microsatellite-based map. The linkage map was used to order and orient the scaffolds from a substantially more contiguous domestic cat genome assembly (Felis catus v8.0), which incorporated ∼20 × coverage of Illumina fragment reads. The new genome assembly shows substantial improvements in contiguity, with a nearly fourfold increase in N50 scaffold size to 18 Mb. We use this map to report probable structural errors in previous maps and assemblies, and to describe features of the recombination landscape, including a massive (∼50 Mb) recombination desert (of virtually zero recombination) on the X chromosome that parallels a similar desert on the porcine X chromosome in both size and physical location. Copyright © 2016 Li et al.

Effects of Print Publication Lag in Dual Format Journals on Scientometric Indicators

PubMed Central

Heneberg, Petr

2013-01-01

Background Publication lag between manuscript submission and its final publication is considered as an important factor affecting the decision to submit, the timeliness of presented data, and the scientometric measures of the particular journal. Dual-format peer-reviewed journals (publishing both print and online editions of their content) adopted a broadly accepted strategy to shorten the publication lag: to publish the accepted manuscripts online ahead of their print editions, which may follow days, but also years later. Effects of this widespread habit on the immediacy index (average number of times an article is cited in the year it is published) calculation were never analyzed. Methodology/Principal Findings Scopus database (which contains nearly up-to-date documents in press, but does not reveal citations by these documents until they are finalized) was searched for the journals with the highest total counts of articles in press, or highest counts of articles in press appearing online in 2010–2011. Number of citations received by the articles in press available online was found to be nearly equal to citations received within the year when the document was assigned to a journal issue. Thus, online publication of in press articles affects severely the calculation of immediacy index of their source titles, and disadvantages online-only and print-only journals when evaluating them according to the immediacy index and probably also according to the impact factor and similar measures. Conclusions/Significance Caution should be taken when evaluating dual-format journals supporting long publication lag. Further research should answer the question, on whether the immediacy index should be replaced by an indicator based on the date of first publication (online or in print, whichever comes first) to eliminate the problems analyzed in this report. Information value of immediacy index is further questioned by very high ratio of authors’ self-citations among the citation window used for its calculation. PMID:23573216

Hierarchical protein export mechanism of the bacterial flagellar type III protein export apparatus.

PubMed

Minamino, Tohru

2018-06-01

The bacterial flagellum is supramolecular motility machinery consisting of the basal body, the hook and the filament. Flagellar proteins are translocated across the cytoplasmic membrane via a type III protein export apparatus, diffuse down the central channel of the growing structure and assemble at the distal end. Flagellar assembly begins with the basal body, followed by the hook and finally the filament. The completion of hook assembly is the most important morphological checkpoint of the sequential flagellar assembly process. When the hook reaches its mature length of about 55 nm in Salmonella enterica, the type III protein export apparatus switches export specificity from proteins required for the structure and assembly of the hook to those responsible for filament assembly, thereby terminating hook assembly and initiating filament assembly. Three flagellar proteins, namely FliK, FlhB and FlhA, are responsible for this substrate specificity switching. Upon completion of the switching event, interactions among FlhA, the cytoplasmic ATPase complex and flagellar type III export chaperones establish the assembly order of the filament at the hook tip. Here, we describe our current understanding of a hierarchical protein export mechanism used in flagellar type III protein export.

Factors in electrode fabrication for performance enhancement of anion exchange membrane water electrolysis

NASA Astrophysics Data System (ADS)

Cho, Min Kyung; Park, Hee-Young; Choe, Seunghoe; Yoo, Sung Jong; Kim, Jin Young; Kim, Hyoung-Juhn; Henkensmeier, Dirk; Lee, So Young; Sung, Yung-Eun; Park, Hyun S.; Jang, Jong Hyun

2017-04-01

To improve the cell performance for alkaline anion exchange membrane water electrolysis (AEMWE), the effects of the amount of polytetrafluoroethylene (PTFE) non-ionomeric binder in the anode and the hot-pressing conditions during the fabrication of the membrane electrode assemblies (MEAs) on cell performances are studied. The electrochemical impedance data indicates that hot-pressing at 50 °C for 1 min during MEA construction can reduce the polarization resistance of AEMWE by ∼12%, and increase the initial water electrolysis current density at 1.8 V (from 195 to 243 mA cm-2). The electrochemical polarization and impedance results also suggest that the AEMWE performance is significantly affected by the content of PTFE binder in the anode electrode, and the optimal content is found to be 9 wt% between 5 and 20 wt%. The AEMWE device fabricated with the optimized parameters exhibits good water splitting performance (299 mA cm-2 at 1.8 V) without noticeable degradation in voltage cycling operations.

Device for preparing combinatorial libraries in powder metallurgy.

PubMed

Yang, Shoufeng; Evans, Julian R G

2004-01-01

This paper describes a powder-metering, -mixing, and -dispensing mechanism that can be used as a method for producing large numbers of samples for metallurgical evaluation or electrical or mechanical testing from multicomponent metal and cermet powder systems. It is designed to make use of the same commercial powders that are used in powder metallurgy and, therefore, to produce samples that are faithful to the microstructure of finished products. The particle assemblies produced by the device could be consolidated by die pressing, isostatic pressing, laser sintering, or direct melting. The powder metering valve provides both on/off and flow rate control of dry powders in open capillaries using acoustic vibration. The valve is simple and involves no relative movement, avoiding seizure with fine powders. An orchestra of such valves can be arranged on a building platform to prepare multicomponent combinatorial libraries. As with many combinatorial devices, identification and evaluation of sources of mixing error as a function of sample size is mandatory. Such an analysis is presented.

Rapid adhesive bonding concepts

NASA Technical Reports Server (NTRS)

Stein, B. A.; Tyeryar, J. R.; Hodges, W. T.

1984-01-01

Adhesive bonding in the aerospace industry typically utilizes autoclaves or presses which have considerable thermal mass. As a consequence, the rates of heatup and cooldown of the bonded parts are limited and the total time and cost of the bonding process is often relatively high. Many of the adhesives themselves do not inherently require long processing times. Bonding could be performed rapidly if the heat was concentrated in the bond lines or at least in the adherends. Rapid adhesive bonding concepts were developed to utilize induction heating techniques to provide heat directly to the bond line and/or adherends without heating the entire structure, supports, and fixtures of a bonding assembly. Bonding times for specimens are cut by a factor of 10 to 100 compared to standard press bonding. The development of rapid adhesive bonding for lap shear specimens (per ASTM D1003 and D3163), for aerospace panel bonding, and for field repair needs of metallic and advanced fiber reinforced polymeric matrix composite structures are reviewed.

Dependence of Halo Bias and Kinematics on Assembly Variables

NASA Astrophysics Data System (ADS)

Xu, Xiaoju; Zheng, Zheng

2018-06-01

Using dark matter haloes identified in a large N-body simulation, we study halo assembly bias, with halo formation time, peak maximum circular velocity, concentration, and spin as the assembly variables. Instead of grouping haloes at fixed mass into different percentiles of each assembly variable, we present the joint dependence of halo bias on the values of halo mass and each assembly variable. In the plane of halo mass and one assembly variable, the joint dependence can be largely described as halo bias increasing outward from a global minimum. We find it unlikely to have a combination of halo variables to absorb all assembly bias effects. We then present the joint dependence of halo bias on two assembly variables at fixed halo mass. The gradient of halo bias does not necessarily follow the correlation direction of the two assembly variables and it varies with halo mass. Therefore in general for two correlated assembly variables one cannot be used as a proxy for the other in predicting halo assembly bias trend. Finally, halo assembly is found to affect the kinematics of haloes. Low-mass haloes formed earlier can have much higher pairwise velocity dispersion than those of massive haloes. In general, halo assembly leads to a correlation between halo bias and halo pairwise velocity distribution, with more strongly clustered haloes having higher pairwise velocity and velocity dispersion. However, the correlation is not tight, and the kinematics of haloes at fixed halo bias still depends on halo mass and assembly variables.

Study of school zones with traffic signals : final report, June 2009.

DOT National Transportation Integrated Search

2009-06-01

One of the most pressing and controversial problems faced by parents, school administrators, law enforcement and transportation professionals throughout the country is the safety of children on their way to and from school. To proactively address thi...

Peptide assembly-driven metal-organic framework (MOF) motors for micro electric generators

DOE PAGES

Ikezoe, Yasuhiro; Fang, Justin; Wasik, Tomasz L.; ...

2014-11-22

Peptide–metal–organic framework (Pep-MOF) motors, whose motions are driven by anisotropic surface tension gradients created via peptide self-assembly around frameworks, can rotate microscopic rotors and magnets fast enough to generate an electric power of 0.1 μW. Finally, a new rigid Pep-MOF motor can be recycled by refilling the peptide fuel into the nanopores of the MOF.

Nanoscale superstructures assembled by polymerase chain reaction (PCR): programmable construction, structural diversity, and emerging applications.

PubMed

Kuang, Hua; Ma, Wei; Xu, Liguang; Wang, Libing; Xu, Chuanlai

2013-11-19

Polymerase chain reaction (PCR) is an essential tool in biotechnology laboratories and is becoming increasingly important in other areas of research. Extensive data obtained over the last 12 years has shown that the combination of PCR with nanoscale dispersions can resolve issues in the preparation DNA-based materials that include both inorganic and organic nanoscale components. Unlike conventional DNA hybridization and antibody-antigen complexes, PCR provides a new, effective assembly platform that both increases the yield of DNA-based nanomaterials and allows researchers to program and control assembly with predesigned parameters including those assisted and automated by computers. As a result, this method allows researchers to optimize to the combinatorial selection of the DNA strands for their nanoparticle conjugates. We have developed a PCR approach for producing various nanoscale assemblies including organic motifs such as small molecules, macromolecules, and inorganic building blocks, such as nanorods (NRs), metal, semiconductor, and magnetic nanoparticles (NPs). We start with a nanoscale primer and then modify that building block using the automated steps of PCR-based assembly including initialization, denaturation, annealing, extension, final elongation, and final hold. The intermediate steps of denaturation, annealing, and extension are cyclic, and we use computer control so that the assembled superstructures reach their predetermined complexity. The structures assembled using a small number of PCR cycles show a lower polydispersity than similar discrete structures obtained by direct hybridization between the nanoscale building blocks. Using different building blocks, we assembled the following structural motifs by PCR: (1) discrete nanostructures (NP dimers, NP multimers including trimers, pyramids, tetramers or hexamers, etc.), (2) branched NP superstructures and heterochains, (3) NP satellite-like superstructures, (4) Y-shaped nanostructures and DNA networks, (5) protein-DNA co-assembly structures, and (6) DNA block copolymers including trimers and pentamers. These results affirm that this method can produce a variety of chemical structures and in yields that are tunable. Using PCR-based preparation of DNA-bridged nanostructures, we can program the assembly of the nanoscale blocks through the adjustment of the primer intensity on the assembled units, the number of PCR cycles, or both. The resulting structures are highly complex and diverse and have interesting dynamics and collective properties. Potential applications of these materials include chirooptical materials, probe fabrication, and environmental and biomedical sensors.

Self-assembled nanomaterials for photoacoustic imaging

NASA Astrophysics Data System (ADS)

Wang, Lei; Yang, Pei-Pei; Zhao, Xiao-Xiao; Wang, Hao

2016-01-01

In recent years, extensive endeavors have been paid to construct functional self-assembled nanomaterials for various applications such as catalysis, separation, energy and biomedicines. To date, different strategies have been developed for preparing nanomaterials with diversified structures and functionalities via fine tuning of self-assembled building blocks. In terms of biomedical applications, bioimaging technologies are urgently calling for high-efficient probes/contrast agents for high-performance bioimaging. Photoacoustic (PA) imaging is an emerging whole-body imaging modality offering high spatial resolution, deep penetration and high contrast in vivo. The self-assembled nanomaterials show high stability in vivo, specific tolerance to sterilization and prolonged half-life stability and desirable targeting properties, which is a kind of promising PA contrast agents for biomedical imaging. Herein, we focus on summarizing recent advances in smart self-assembled nanomaterials with NIR absorption as PA contrast agents for biomedical imaging. According to the preparation strategy of the contrast agents, the self-assembled nanomaterials are categorized into two groups, i.e., the ex situ and in situ self-assembled nanomaterials. The driving forces, assembly modes and regulation of PA properties of self-assembled nanomaterials and their applications for long-term imaging, enzyme activity detection and aggregation-induced retention (AIR) effect for diagnosis and therapy are emphasized. Finally, we conclude with an outlook towards future developments of self-assembled nanomaterials for PA imaging.

Self-assembled nanomaterials for photoacoustic imaging.

PubMed

Wang, Lei; Yang, Pei-Pei; Zhao, Xiao-Xiao; Wang, Hao

2016-02-07

In recent years, extensive endeavors have been paid to construct functional self-assembled nanomaterials for various applications such as catalysis, separation, energy and biomedicines. To date, different strategies have been developed for preparing nanomaterials with diversified structures and functionalities via fine tuning of self-assembled building blocks. In terms of biomedical applications, bioimaging technologies are urgently calling for high-efficient probes/contrast agents for high-performance bioimaging. Photoacoustic (PA) imaging is an emerging whole-body imaging modality offering high spatial resolution, deep penetration and high contrast in vivo. The self-assembled nanomaterials show high stability in vivo, specific tolerance to sterilization and prolonged half-life stability and desirable targeting properties, which is a kind of promising PA contrast agents for biomedical imaging. Herein, we focus on summarizing recent advances in smart self-assembled nanomaterials with NIR absorption as PA contrast agents for biomedical imaging. According to the preparation strategy of the contrast agents, the self-assembled nanomaterials are categorized into two groups, i.e., the ex situ and in situ self-assembled nanomaterials. The driving forces, assembly modes and regulation of PA properties of self-assembled nanomaterials and their applications for long-term imaging, enzyme activity detection and aggregation-induced retention (AIR) effect for diagnosis and therapy are emphasized. Finally, we conclude with an outlook towards future developments of self-assembled nanomaterials for PA imaging.

Deviation diagnosis and analysis of hull flat block assembly based on a state space model

NASA Astrophysics Data System (ADS)

Zhang, Zhiying; Dai, Yinfang; Li, Zhen

2012-09-01

Dimensional control is one of the most important challenges in the shipbuilding industry. In order to predict assembly dimensional variation in hull flat block construction, a variation stream model based on state space was presented in this paper which can be further applied to accuracy control in shipbuilding. Part accumulative error, locating error, and welding deformation were taken into consideration in this model, and variation propagation mechanisms and the accumulative rule in the assembly process were analyzed. Then, a model was developed to describe the variation propagation throughout the assembly process. Finally, an example of flat block construction from an actual shipyard was given. The result shows that this method is effective and useful.

Power Reactant Storage Assembly (PRSA) (Space Shuttle). PRSA hydrogen and oxygen DVT tank refurbishment

NASA Technical Reports Server (NTRS)

1993-01-01

The Power Reactant Storage Assembly (PRSA) liquid hydrogen Development Verification Test (H2 DVT) tank assembly (Beech Aircraft Corporation P/N 15548-0116-1, S/N 07399000SHT0001) and liquid oxygen (O2) DVT tank assembly (Beech Aircraft Corporation P/N 15548-0115-1, S/N 07399000SXT0001) were refurbished by Ball Electro-Optics and Cryogenics Division to provide NASA JSC, Propulsion and Power Division, the capability of performing engineering tests. The refurbishments incorporated the latest flight configuration hardware and avionics changes necessary to make the tanks function like flight articles. This final report summarizes these refurbishment activities. Also included are up-to-date records of the pressure time and cycle histories.

ACL reconstruction using bone-patella tendon-bone autograft: press-fit technique vs. interference screw fixation.

PubMed

Sarzaeem, M M; Najafi, F; Razi, M; Najafi, M A

2014-07-01

The gold standard in ACL reconstructions has been the bone-patellar tendon-bone autograft fixed with interference screws. This prospective, randomized clinical trial aimed to compare two methods of fixation for BPTB grafts: press-fit fixation vs. interference screw, over a 12-month follow-up interval. 158 patients with an average age of 29.8 years, between 2011 and 2012, were treated for torn ACL. 82 patients underwent reconstruction with BPTB autograft with a press fit fixation technique, and in 76 cases an interference screw was used. At the time of final follow-up, 71 patients in press-fit group and 65 patients in interference screw group were evaluated in terms of return to pre-injury activity level, pain, knee stability, range of motion, IKDC score and complications. At 12-month follow-up, 59 (83 %) and 55 (85 %) in press-fit and screw group, respectively had good-to-excellent IKDC score (p > 0.05). The mean laxity assessed using a KT-1000 arthrometer improved to 2.7 and 2.5 mm in press-fit and screw group, respectively. Regarding Lachman and pivot shift test, there was a statistically significant improvement in the integrity of the ACL in both the groups, but no significant differences was noted between groups. There were no significant differences in terms of femur circumference difference, effusion, knee range of motion, pain and complications. The press-fit technique is an efficient procedure. Its outcome was comparable with the interference screw group. Furthermore it has unlimited bone-to-bone healing, no need for removal of hardware, ease for revision and cost effectiveness.

Alkaline-assisted screw press pretreatment affecting enzymatic hydrolysis of wheat straw.

PubMed

Yan, Qingqi; Wang, Yumei; Rodiahwati, Wawat; Spiess, Antje; Modigell, Michael

2017-02-01

Screw press processing of biomass can be considered as a suitable mechanically based pretreatment for biofuel production since it disrupts the structure of lignocellulosic biomass with high shear and pressure forces. The combination with chemical treatment has been suggested to increase the conversion of lignocellulosic biomass to fermentable sugars. Within the study, the synergetic effect of alkaline (sodium hydroxide, NaOH) soaking and screw press pretreatment on wheat straw was evaluated based on, e.g., sugar recovery and energy efficiency. After alkaline soaking (at 0.1 M for 30 min) and sequential screw press pretreatment with various screw press configurations and modified screw barrel, the lignin content of pretreated wheat straw was quantified. In addition, the structure of pretreated wheat straw was investigated by scanning electron microscopy and measurement of specific surface area. It could be shown that removal of lignin is more important than increase of surface area of the biomass to reach a high sugar recovery. The rate constant of the enzymatic hydrolysis increased from 1.1 × 10 -3  1/h for the non-treated material over 2.3 × 10 -3  1/h for the alkaline-soaked material to 26.9 × 10 -3  1/h for alkaline-assisted screw press pretreated material, indicating a nearly 25-fold improvement of the digestibility by the combined chemo-mechanical pretreatment. Finally, the screw configuration was found to be an important factor for improving the sugar recovery and for reducing the specific energy consumption of the screw press pretreatment.

Strength and microstructure of IPS Empress 2 glass-ceramic after different treatments.

PubMed

Oh, S C; Dong, J K; Lüthy, H; Schärer, P

2000-01-01

This investigation was designed to determine whether heat pressing and/or simulated heat treatments affect the flexure strength and microstructure of the lithium disilicate glass-ceramic of the IPS Empress 2 system. Four groups of the lithium disilicate glass-ceramic were prepared as follows: group 1 = as-received material; group 2 = heat-pressed material; group 3 = heat-pressed and stimulated initial heat-treated material; and group 4 = heat-pressed and simulated heat-treated material with full firings for a final restoration. Three-point bending tests and scanning electron microscopy (SEM) analysis were conducted. The flexure strength of group 2 was significantly higher than that of group 1. However, there were no significant differences in strength among groups 2, 3, and 4, or between groups 1 and 4. The SEM micrographs of the lithium disilicate glass-ceramic showed a closely packed, multidirectionally interlocking microstructure of numerous lithium disilicate crystals protruding from the glass matrix. The crystals in the glass matrix of the heat-pressed materials (groups 2, 3, and 4) were a little more homogeneous and about 2 times bigger than those of the as-received material (group 1). These changes of the microstructure were greatest between groups 1 and 2. However, there were no marked differences among groups 2, 3, and 4. Although there were significant increases in the strength and some changes of the microstructure after the heat-pressing operation, the combination of heat pressing and simulated subsequent heat treatments did not produce an increase of strength of IPS Empress 2 glass-ceramic.

Superplastic forging nitride ceramics

DOEpatents

Panda, P.C.; Seydel, E.R.; Raj, R.

1988-03-22

A process is disclosed for preparing silicon nitride ceramic parts which are relatively flaw free and which need little or no machining, said process comprising the steps of: (a) preparing a starting powder by wet or dry mixing ingredients comprising by weight from about 70% to about 99% silicon nitride, from about 1% to about 30% of liquid phase forming additive and from 1% to about 7% free silicon; (b) cold pressing to obtain a preform of green density ranging from about 30% to about 75% of theoretical density; (c) sintering at atmospheric pressure in a nitrogen atmosphere at a temperature ranging from about 1,400 C to about 2,200 C to obtain a density which ranges from about 50% to about 100% of theoretical density and which is higher than said preform green density, and (d) press forging workpiece resulting from step (c) by isothermally uniaxially pressing said workpiece in an open die without initial contact between said workpiece and die wall perpendicular to the direction of pressing and so that pressed workpiece does not contact die wall perpendicular to the direction of pressing, to substantially final shape in a nitrogen atmosphere utilizing a temperature within the range of from about 1,400 C to essentially 1,750 C and strain rate within the range of about 10[sup [minus]7] to about 10[sup [minus]1] seconds[sup [minus]1], the temperature and strain rate being such that surface cracks do not occur, said pressing being carried out to obtain a shear deformation greater than 30% whereby superplastic forging is effected.

Self-assembly strategies for the synthesis of functional nanostructured materials

NASA Astrophysics Data System (ADS)

Perego, M.; Seguini, G.

2016-06-01

Self-assembly is the autonomous organization of components into patterns or structures without human intervention. This is the approach followed by nature to generate living cells and represents one of the practical strategies to fabricate ensembles of nanostructures. In static self-assembly the formation of ordered structures could require energy but once formed the structures are stable. The introduction of additional regular features in the environment could be used to template the self-assembly guiding the organization of the components and determining the final structure they form. In this regard self-assembly of block copolymers represents a potent platform for fundamental studies at the nanoscale and for application-driven investigation as a tool to fabricate functional nanostructured materials. Block copolymers can hierarchically assemble into chemically distinct domains with size and periodicity on the order of 10nm or below, offering a potentially inexpensive route to generate large-area nanostructured materials. The final structure characteristics of these materials are dictated by the properties of the elementary block copolymers, like chain length, volume fraction or degree of block incompatibility. Modern synthetic chemistry offers the possibility to design these macromolecules with very specific length scales and geometries, directly embodying in the block copolymers the code that drives their self- assembling process. The understanding of the kinetics and thermodynamics of the block copolymer self-assembly process in the bulk phase as well as in thin films represents a fundamental prerequisite toward the exploitation of these materials. Incorporating block copolymer into device fabrication procedures or directly into devices, as active elements, will lead to the development of a new generation of devices fabricated using the fundamental law of nature to our advantage in order to minimize cost and power consumption in the fabrication process. Moreover the capability to precisely organize these nano-objects on appropriate substrates is the key point to support the technological development of new device concepts with predictable characteristics based on these nano-materials. In the next coming years this area of research, at the intersection between fundamental science and technology, is expected to disclose additional insights in the physics of the self-assembly process and to delineate unforeseen applications for these exciting materials.

Influence of Winemaking Processing Steps on the Amounts of (E)-2-Decenal and Tridecane as Off-Odorants Caused by Brown Marmorated Stink Bug (Halyomorpha halys).

PubMed

Mohekar, Pallavi; Osborne, James; Wiman, Nik G; Walton, Vaughn; Tomasino, Elizabeth

2017-02-01

Brown marmorated stink bugs (BMSB) release stress compounds, tridecane and (E)-2-decenal, that affect final wine quality. This study focuses on determining the effect of wine processing on (E)-2-decenal and tridecane release in both red and white wines. Wines were produced by adding live BMSB to grape clusters at densities of 0, 0.3, 1, and 3 bugs/cluster. Compound concentrations were measured using headspace solid phase microextraction with multidimensional gas chromatography and mass spectrometry. For red wines, the highest levels of stress compounds were found using 3 BMSB/cluster [tridecane, 614 μg/L; (E)-2-decenal, 2.0 μg/L]. Pressing was found to be the critical process point for stress compound release, and additional pressing processes, press types, and press fractions were investigated. BMSB taint for white wines was not found to be problematic with respect to wine quality. An action control of 3 BMSB/cluster is recommended as this was related to the known consumption rejection threshold for (E)-2-decenal.

Zirconium Carbide Produced by Spark Plasma Sintering and Hot Pressing: Densification Kinetics, Grain Growth, and Thermal Properties

PubMed Central

Wei, Xialu; Back, Christina; Izhvanov, Oleg; Haines, Christopher D.; Olevsky, Eugene A.

2016-01-01

Spark plasma sintering (SPS) has been employed to consolidate a micron-sized zirconium carbide (ZrC) powder. ZrC pellets with a variety of relative densities are obtained under different processing parameters. The densification kinetics of ZrC powders subjected to conventional hot pressing and SPS are comparatively studied by applying similar heating and loading profiles. Due to the lack of electric current assistance, the conventional hot pressing appears to impose lower strain rate sensitivity and higher activation energy values than those which correspond to the SPS processing. A finite element simulation is used to analyze the temperature evolution within the volume of ZrC specimens subjected to SPS. The control mechanism for grain growth during the final SPS stage is studied via a recently modified model, in which the grain growth rate dependence on porosity is incorporated. The constant pressure specific heat and thermal conductivity of the SPS-processed ZrC are determined to be higher than those reported for the hot-pressed ZrC and the benefits of applying SPS are indicated accordingly. PMID:28773697

Microstructural Effects on Initiation Behavior in HMX

NASA Astrophysics Data System (ADS)

Molek, Christopher; Welle, Eric; Hardin, Barrett; Vitarelli, Jim; Wixom, Ryan; Samuels, Philip

Understanding the role microstructure plays on ignition and growth behavior has been the subject of a significant body of research within the detonation physics community. The pursuit of this understanding is important because safety and performance characteristics have been shown to strongly correlate to particle morphology. Historical studies have often correlated bulk powder characteristics to the performance or safety characteristics of pressed materials. We believe that a clearer and more relevant correlation is made between the pressed microstructure and the observed detonation behavior. This type of assessment is possible, as techniques now exist for the quantification of the pressed microstructures. Our talk will report on experimental efforts that correlate directly measured microstructural characteristics to initiation threshold behavior of HMX based materials. The internal microstructures were revealed using an argon ion cross-sectioning technique. This technique enabled the quantification of density and interface area of the pores within the pressed bed using methods of stereology. These bed characteristics are compared to the initiation threshold behavior of three HMX based materials using an electric gun based test method. Finally, a comparison of experimental threshold data to supporting theoretical efforts will be made.

Naval Remote Ocean Sensing System (NROSS) study

NASA Technical Reports Server (NTRS)

1983-01-01

A set of hardware similar to the SEASAT A configuration requirement, suitable for installation and operation aboard a NOAA-D bus and a budgetary cost for one (1) protoflight model was provided. The scatterometer sensor is conceived as one of several sensors for the Navy Remote Ocean Sensing System (NROSS) Satellite Program. Deliverables requested were to include a final report with appropriate sketches and block diagrams showing the scatterometer design/configuration and a budgetary cost for all labor and materials to design, fabricate, test, and integrate this hardware into a NOAA-D satellite bus. This configuration consists of two (2) hardware assembles - a transmitter/receiver (T/R) assembly and an integrated electronics assembly (IEA). The T/R assembly as conceived is best located at the extreme opposite end of the satellite away from the solar array assembly and oriented in position to enable one surface of the assembly to have unobstructed exposure to space. The IEA is planned to be located at the bottom (Earth viewing) side of the satellite and requires a radiating plate.

Printable Functional Chips Based on Nanoparticle Assembly.

PubMed

Huang, Yu; Li, Wenbo; Qin, Meng; Zhou, Haihua; Zhang, Xingye; Li, Fengyu; Song, Yanlin

2017-01-01

With facile manufacturability and modifiability, impressive nanoparticles (NPs) assembly applications were performed for functional patterned devices, which have attracted booming research attention due to their increasing applications in high-performance optical/electrical devices for sensing, electronics, displays, and catalysis. By virtue of easy and direct fabrication to desired patterns, high throughput, and low cost, NPs assembly printing is one of the most promising candidates for the manufacturing of functional micro-chips. In this review, an overview of the fabrications and applications of NPs patterned assembly by printing methods, including inkjet printing, lithography, imprinting, and extended printing techniques is presented. The assembly processes and mechanisms on various substrates with distinct wettabilities are deeply discussed and summarized. Via manipulating the droplet three phase contact line (TCL) pinning or slipping, the NPs contracted in ink are controllably assembled following the TCL, and generate novel functional chips and correlative integrate devices. Finally, the perspective of future developments and challenges is presented and widely exhibited. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A comparison across non-model animals suggests an optimal sequencing depth for de novo transcriptome assembly

PubMed Central

2013-01-01

Background The lack of genomic resources can present challenges for studies of non-model organisms. Transcriptome sequencing offers an attractive method to gather information about genes and gene expression without the need for a reference genome. However, it is unclear what sequencing depth is adequate to assemble the transcriptome de novo for these purposes. Results We assembled transcriptomes of animals from six different phyla (Annelids, Arthropods, Chordates, Cnidarians, Ctenophores, and Molluscs) at regular increments of reads using Velvet/Oases and Trinity to determine how read count affects the assembly. This included an assembly of mouse heart reads because we could compare those against the reference genome that is available. We found qualitative differences in the assemblies of whole-animals versus tissues. With increasing reads, whole-animal assemblies show rapid increase of transcripts and discovery of conserved genes, while single-tissue assemblies show a slower discovery of conserved genes though the assembled transcripts were often longer. A deeper examination of the mouse assemblies shows that with more reads, assembly errors become more frequent but such errors can be mitigated with more stringent assembly parameters. Conclusions These assembly trends suggest that representative assemblies are generated with as few as 20 million reads for tissue samples and 30 million reads for whole-animals for RNA-level coverage. These depths provide a good balance between coverage and noise. Beyond 60 million reads, the discovery of new genes is low and sequencing errors of highly-expressed genes are likely to accumulate. Finally, siphonophores (polymorphic Cnidarians) are an exception and possibly require alternate assembly strategies. PMID:23496952

A comparison across non-model animals suggests an optimal sequencing depth for de novo transcriptome assembly.

PubMed

Francis, Warren R; Christianson, Lynne M; Kiko, Rainer; Powers, Meghan L; Shaner, Nathan C; Haddock, Steven H D

2013-03-12

The lack of genomic resources can present challenges for studies of non-model organisms. Transcriptome sequencing offers an attractive method to gather information about genes and gene expression without the need for a reference genome. However, it is unclear what sequencing depth is adequate to assemble the transcriptome de novo for these purposes. We assembled transcriptomes of animals from six different phyla (Annelids, Arthropods, Chordates, Cnidarians, Ctenophores, and Molluscs) at regular increments of reads using Velvet/Oases and Trinity to determine how read count affects the assembly. This included an assembly of mouse heart reads because we could compare those against the reference genome that is available. We found qualitative differences in the assemblies of whole-animals versus tissues. With increasing reads, whole-animal assemblies show rapid increase of transcripts and discovery of conserved genes, while single-tissue assemblies show a slower discovery of conserved genes though the assembled transcripts were often longer. A deeper examination of the mouse assemblies shows that with more reads, assembly errors become more frequent but such errors can be mitigated with more stringent assembly parameters. These assembly trends suggest that representative assemblies are generated with as few as 20 million reads for tissue samples and 30 million reads for whole-animals for RNA-level coverage. These depths provide a good balance between coverage and noise. Beyond 60 million reads, the discovery of new genes is low and sequencing errors of highly-expressed genes are likely to accumulate. Finally, siphonophores (polymorphic Cnidarians) are an exception and possibly require alternate assembly strategies.

Contractile-Ring Assembly in Fission Yeast Cytokinesis: Recent Advances and New Perspectives

PubMed Central

Lee, I-Ju; Coffman, Valerie C.; Wu, Jian-Qiu

2017-01-01

The fission yeast Schizosaccharomyces pombe is an excellent model organism to study cytokinesis. Here, we review recent advances on contractile-ring assembly in fission yeast. First, we summarize the assembly of cytokinesis nodes, the precursors of a normal contractile ring. IQGAP Rng2 and myosin essential light chain Cdc4 are recruited by the anillin-like protein Mid1, followed by the addition of other cytokinesis node proteins. Mid1 localization on the plasma membrane is stabilized by interphase node proteins. Second, we discuss proteins and processes that contribute to the search, capture, pull, and release mechanism of contractile-ring assembly. Actin filaments nucleated by formin Cdc12, the motor activity of myosin-II, the stiffness of the actin network, and severing of actin filaments by cofilin all play essential roles in contractile-ring assembly. Finally, we discuss the Mid1-independent pathway for ring assembly, and the possible mechanisms underlying the ring maturation and constriction. Collectively, we provide an overview of the current understanding of contractile-ring assembly and uncover future directions in studying cytokinesis in fission yeast. PMID:22887981

Contractile-ring assembly in fission yeast cytokinesis: Recent advances and new perspectives.

PubMed

Lee, I-Ju; Coffman, Valerie C; Wu, Jian-Qiu

2012-10-01

The fission yeast Schizosaccharomyces pombe is an excellent model organism to study cytokinesis. Here, we review recent advances on contractile-ring assembly in fission yeast. First, we summarize the assembly of cytokinesis nodes, the precursors of a normal contractile ring. IQGAP Rng2 and myosin essential light chain Cdc4 are recruited by the anillin-like protein Mid1, followed by the addition of other cytokinesis node proteins. Mid1 localization on the plasma membrane is stabilized by interphase node proteins. Second, we discuss proteins and processes that contribute to the search, capture, pull, and release mechanism of contractile-ring assembly. Actin filaments nucleated by formin Cdc12, the motor activity of myosin-II, the stiffness of the actin network, and severing of actin filaments by cofilin all play essential roles in contractile-ring assembly. Finally, we discuss the Mid1-independent pathway for ring assembly, and the possible mechanisms underlying the ring maturation and constriction. Collectively, we provide an overview of the current understanding of contractile-ring assembly and uncover future directions in studying cytokinesis in fission yeast. Copyright © 2012 Wiley Periodicals, Inc.

Engineering a Quieter America: Commercial Aviation: A New Era - Workshop Final Report

DOT National Transportation Integrated Search

2017-09-01

The report Technology for a Quieter America1 (TQA), published in 2010 by the National Academies Press on behalf of the National Academy of Engineering (NAE), emphasizes the importance of engineeringand, in particular, the role of noise control tec...

New Insight into Sugarcane Industry Waste Utilization (Press Mud) for Cleaner Biobutanol Production by Using C. acetobutylicum NRRL B-527.

PubMed

Nimbalkar, Pranhita R; Khedkar, Manisha A; Gaikwad, Shashank G; Chavan, Prakash V; Bankar, Sandip B

2017-11-01

In the present study, press mud, a sugar industry waste, was explored for biobutanol production to strengthen agricultural economy. The fermentative production of biobutanol was investigated via series of steps, viz. characterization, drying, acid hydrolysis, detoxification, and fermentation. Press mud contains an adequate amount of cellulose (22.3%) and hemicellulose (21.67%) on dry basis, and hence, it can be utilized for further acetone-butanol-ethanol (ABE) production. Drying experiments were conducted in the temperature range of 60-120 °C to circumvent microbial spoilage and enhance storability of press mud. Furthermore, acidic pretreatment variables, viz. sulfuric acid concentration, solid to liquid ratio, and time, were optimized using response surface methodology. The corresponding values were found to be 1.5% (v/v), 1:5 g/mL, and 15 min, respectively. In addition, detoxification studies were also conducted using activated charcoal, which removed almost 93-97% phenolics and around 98% furans, which are toxic to microorganisms during fermentation. Finally, the batch fermentation of detoxified press mud slurry (the sample dried at 100 °C and pretreated) using Clostridium acetobutylicum NRRL B-527 resulted in a higher butanol production of 4.43 g/L with a total ABE of 6.69 g/L.

Using Neutron Diffraction to Investigate Texture Evolution During Consolidation of Deuterated Triaminotrinitrobenzene (d-TATB) Explosive Powder

DOE PAGES

Luscher, Darby J.; Yeager, John D.; Clausen, Bjørn; ...

2017-05-14

Triaminotrinitrobenzene (TATB) is a highly anisotropic molecular crystal used in several plastic-bonded explosive (PBX) formulations. A complete understanding of the orientation distribution of TATB particles throughout a PBX charge is required to understand spatially variable, anisotropic macroscale properties of the charge. Although texture of these materials can be measured after they have been subjected to mechanical or thermal loads, measuring texture evolution in situ is important in order to identify mechanisms of crystal deformation and reorientation used to better inform thermomechanical models. Neutron diffraction measurements were used to estimate crystallographic reorientation while deuterated TATB (d-TATB) powder was consolidated into amore » cylindrical pellet via a uniaxial die-pressing operation at room temperature. Both the final texture of the pressed pellet and the in situ evolution of texture during pressing were measured, showing that the d-TATB grains reorient such that (001) poles become preferentially aligned with the pressing direction. A compaction model is used to predict the evolution of texture in the pellet during the pressing process, finding that the original model overpredicted the texture strength compared to these measurements. The theory was extended to account for initial particle shape and pore space, bringing the results into good agreement with the data.« less

Using Neutron Diffraction to Investigate Texture Evolution During Consolidation of Deuterated Triaminotrinitrobenzene (d-TATB) Explosive Powder

DOE Office of Scientific and Technical Information (OSTI.GOV)

Luscher, Darby J.; Yeager, John D.; Clausen, Bjørn

Triaminotrinitrobenzene (TATB) is a highly anisotropic molecular crystal used in several plastic-bonded explosive (PBX) formulations. A complete understanding of the orientation distribution of TATB particles throughout a PBX charge is required to understand spatially variable, anisotropic macroscale properties of the charge. Although texture of these materials can be measured after they have been subjected to mechanical or thermal loads, measuring texture evolution in situ is important in order to identify mechanisms of crystal deformation and reorientation used to better inform thermomechanical models. Neutron diffraction measurements were used to estimate crystallographic reorientation while deuterated TATB (d-TATB) powder was consolidated into amore » cylindrical pellet via a uniaxial die-pressing operation at room temperature. Both the final texture of the pressed pellet and the in situ evolution of texture during pressing were measured, showing that the d-TATB grains reorient such that (001) poles become preferentially aligned with the pressing direction. A compaction model is used to predict the evolution of texture in the pellet during the pressing process, finding that the original model overpredicted the texture strength compared to these measurements. The theory was extended to account for initial particle shape and pore space, bringing the results into good agreement with the data.« less

Self-assembling peptide-based building blocks in medical applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Acar, Handan; Srivastava, Samanvaya; Chung, Eun Ji

Peptides and peptide-conjugates, comprising natural and synthetic building blocks, are an increasingly popular class of biomaterials. Self-assembled nanostructures based on peptides and peptide-conjugates offer advantages such as precise selectivity and multifunctionality that can address challenges and limitations in the clinic. In this review article, we discuss recent developments in the design and self-assembly of various nanomaterials based on peptides and peptide-conjugates for medical applications, and categorize them into two themes based on the driving forces of molecular self-assembly. First, we present the self-assembled nanostructures driven by the supramolecular interactions between the peptides, with or without the presence of conjugates. Themore » studies where nanoassembly is driven by the interactions between the conjugates of peptide-conjugates are then presented. Particular emphasis is given to in vivo studies focusing on therapeutics, diagnostics, immune modulation and regenerative medicine. Finally, challenges and future perspectives are presented.« less

Gel phase formation in dilute triblock copolyelectrolyte complexes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Srivastava, Samanvaya; Andreev, Marat; Levi, Adam E.

Assembly of oppositely charged triblock copolyelectrolytes into phase-separated gels at low polymer concentrations (<1% by mass) has been observed in scattering experiments and molecular dynamics simulations. Here we show that in contrast to uncharged, amphiphilic block copolymers that form discrete micelles at low concentrations and enter a phase of strongly interacting micelles in a gradual manner with increasing concentration, the formation of a dilute phase of individual micelles is prevented in polyelectrolyte complexation-driven assembly of triblock copolyelectrolytes. Gel phases form and phase separate almost instantaneously on solvation of the copolymers. Furthermore, molecular models of self-assembly demonstrate the presence of oligo-chainmore » aggregates in early stages of copolyelectrolyte assembly, at experimentally unobservable polymer concentrations. Finally, our discoveries contribute to the fundamental understanding of the structure and pathways of complexation-driven assemblies, and raise intriguing prospects for gel formation at extraordinarily low concentrations, with applications in tissue engineering, agriculture, water purification and theranostics.« less

Gel phase formation in dilute triblock copolyelectrolyte complexes

DOE PAGES

Srivastava, Samanvaya; Andreev, Marat; Levi, Adam E.; ...

2017-02-23

Assembly of oppositely charged triblock copolyelectrolytes into phase-separated gels at low polymer concentrations (<1% by mass) has been observed in scattering experiments and molecular dynamics simulations. Here we show that in contrast to uncharged, amphiphilic block copolymers that form discrete micelles at low concentrations and enter a phase of strongly interacting micelles in a gradual manner with increasing concentration, the formation of a dilute phase of individual micelles is prevented in polyelectrolyte complexation-driven assembly of triblock copolyelectrolytes. Gel phases form and phase separate almost instantaneously on solvation of the copolymers. Furthermore, molecular models of self-assembly demonstrate the presence of oligo-chainmore » aggregates in early stages of copolyelectrolyte assembly, at experimentally unobservable polymer concentrations. Finally, our discoveries contribute to the fundamental understanding of the structure and pathways of complexation-driven assemblies, and raise intriguing prospects for gel formation at extraordinarily low concentrations, with applications in tissue engineering, agriculture, water purification and theranostics.« less

A virtual maintenance-based approach for satellite assembling and troubleshooting assessment

NASA Astrophysics Data System (ADS)

Geng, Jie; Li, Ying; Wang, Ranran; Wang, Zili; Lv, Chuan; Zhou, Dong

2017-09-01

In this study, a Virtual Maintenance (VM)-based approach for satellite troubleshooting assessment is proposed. By focusing on various elements in satellite assemble troubleshooting, such as accessibility, ergonomics, wiring, and extent of damage, a systematic, quantitative, and objective assessment model is established to decrease subjectivity in satellite assembling and troubleshooting assessment. Afterwards, based on the established assessment model and satellite virtual prototype, an application process of this model suitable for a virtual environment is presented. Finally, according to the application process, all the elements in satellite troubleshooting are analyzed and assessed. The corresponding improvements, which realize the transformation from a conventional way to a virtual simulation and assessment, are suggested, and the flaws in assembling and troubleshooting are revealed. Assembling or troubleshooting schemes can be improved in the early stage of satellite design with the help of a virtual prototype. Repetition in the practical operation is beneficial to companies as risk and cost are effectively reduced.

Architecture, Assembly, and Emerging Applications of Branched Functional Polyelectrolytes and Poly(ionic liquid)s.

PubMed

Xu, Weinan; Ledin, Petr A; Shevchenko, Valery V; Tsukruk, Vladimir V

2015-06-17

Branched polyelectrolytes with cylindrical brush, dendritic, hyperbranched, grafted, and star architectures bearing ionizable functional groups possess complex and unique assembly behavior in solution at surfaces and interfaces as compared to their linear counterparts. This review summarizes the recent developments in the introduction of various architectures and understanding of the assembly behavior of branched polyelectrolytes with a focus on functional polyelectrolytes and poly(ionic liquid)s with responsive properties. The branched polyelectrolytes and poly(ionic liquid)s interact electrostatically with small molecules, linear polyelectrolytes, or other branched polyelectrolytes to form assemblies of hybrid nanoparticles, multilayer thin films, responsive microcapsules, and ion-conductive membranes. The branched structures lead to unconventional assemblies and complex hierarchical structures with responsive properties as summarized in this review. Finally, we discuss prospectives for emerging applications of branched polyelectrolytes and poly(ionic liquid)s for energy harvesting and storage, controlled delivery, chemical microreactors, adaptive surfaces, and ion-exchange membranes.

Natural supramolecular building blocks: from virus coat proteins to viral nanoparticles.

PubMed

Liu, Zhi; Qiao, Jing; Niu, Zhongwei; Wang, Qian

2012-09-21

Viruses belong to a fascinating class of natural supramolecular structures, composed of multiple copies of coat proteins (CPs) that assemble into different shapes with a variety of sizes from tens to hundreds of nanometres. Because of their advantages including simple/economic production, well-defined structural features, unique shapes and sizes, genetic programmability and robust chemistries, recently viruses and virus-like nanoparticles (VLPs) have been used widely in biomedical applications and materials synthesis. In this critical review, we highlight recent advances in the use of virus coat proteins (VCPs) and viral nanoparticles (VNPs) as building blocks in self-assembly studies and materials development. We first discuss the self-assembly of VCPs into VLPs, which can efficiently incorporate a variety of different materials as cores inside the viral protein shells. Then, the self-assembly of VNPs at surfaces or interfaces is summarized. Finally, we discuss the co-assembly of VNPs with different functional materials (178 references).

Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy

PubMed Central

Ladoux, Benoit; Quivy, Jean-Pierre; Doyle, Patrick; Roure, Olivia du; Almouzni, Geneviève; Viovy, Jean-Louis

2000-01-01

Fluorescence videomicroscopy and scanning force microscopy were used to follow, in real time, chromatin assembly on individual DNA molecules immersed in cell-free systems competent for physiological chromatin assembly. Within a few seconds, molecules are already compacted into a form exhibiting strong similarities to native chromatin fibers. In these extracts, the compaction rate is more than 100 times faster than expected from standard biochemical assays. Our data provide definite information on the forces involved (a few piconewtons) and on the reaction path. DNA compaction as a function of time revealed unique features of the assembly reaction in these extracts. They imply a sequential process with at least three steps, involving DNA wrapping as the final event. An absolute and quantitative measure of the kinetic parameters of the early steps in chromatin assembly under physiological conditions could thus be obtained. PMID:11114182

Mechanisms of Size Control and Polymorphism in Viral Capsid Assembly

PubMed Central

Elrad, Oren M.; Hagan, Michael F.

2009-01-01

We simulate the assembly dynamics of icosahedral capsids from subunits that interconvert between different conformations (or quasi-equivalent states). The simulations identify mechanisms by which subunits form empty capsids with only one morphology, but adaptively assemble into different icosahedral morphologies around nanoparticle cargoes with varying sizes, as seen in recent experiments with brome mosaic virus (BMV) capsid proteins. Adaptive cargo encapsidation requires moderate cargo-subunit interaction strengths; stronger interactions frustrate assembly by stabilizing intermediates with incommensurate curvature. We compare simulation results to experiments with cowpea chlorotic mottle virus empty capsids and BMV capsids assembled on functionalized nanoparticles, and suggest new cargo encapsidation experiments. Finally, we find that both empty and templated capsids maintain the precise spatial ordering of subunit conformations seen in the crystal structure even if interactions that preserve this arrangement are favored by as little as the thermal energy, consistent with experimental observations that different subunit conformations are highly similar. PMID:18950240

An insight into polymerization-induced self-assembly by dissipative particle dynamics simulation.

PubMed

Huang, Feng; Lv, Yisheng; Wang, Liquan; Xu, Pengxiang; Lin, Jiaping; Lin, Shaoliang

2016-08-14

Polymerization-induced self-assembly is a one-pot route to produce concentrated dispersions of block copolymer nano-objects. Herein, dissipative particle dynamics simulations with a reaction model were employed to investigate the behaviors of polymerization-induced self-assembly. The polymerization kinetics in the polymerization-induced self-assembly were analyzed by comparing with solution polymerization. It was found that the polymerization rate enhances in the initial stage and decreases in the later stage. In addition, the effects of polymerization rate, length of macromolecular initiators, and concentration on the aggregate morphologies and formation pathway were studied. The polymerization rate and the length of the macromolecular initiators are found to have a marked influence on the pathway of the aggregate formations and the final structures. Morphology diagrams were mapped correspondingly. A comparison between simulation results and experimental findings is also made and an agreement is shown. This work can enrich our knowledge about polymerization-induced self-assembly.

Communication: Theoretical prediction of free-energy landscapes for complex self-assembly

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jacobs, William M.; Reinhardt, Aleks; Frenkel, Daan

2015-01-14

We present a technique for calculating free-energy profiles for the nucleation of multicomponent structures that contain as many species as building blocks. We find that a key factor is the topology of the graph describing the connectivity of the target assembly. By considering the designed interactions separately from weaker, incidental interactions, our approach yields predictions for the equilibrium yield and nucleation barriers. These predictions are in good agreement with corresponding Monte Carlo simulations. We show that a few fundamental properties of the connectivity graph determine the most prominent features of the assembly thermodynamics. Surprisingly, we find that polydispersity in themore » strengths of the designed interactions stabilizes intermediate structures and can be used to sculpt the free-energy landscape for self-assembly. Finally, we demonstrate that weak incidental interactions can preclude assembly at equilibrium due to the combinatorial possibilities for incorrect association.« less

Effect of heterocyclic capping groups on the self-assembly of a dipeptide hydrogel.

PubMed

Martin, Adam D; Wojciechowski, Jonathan P; Warren, Holly; in het Panhuis, Marc; Thordarson, Pall

2016-03-14

The mechanism and design rules associated with the self-assembly of short peptides into hydrogels is currently not well understood. In this work, four diphenylalanine-based peptides have been synthesised, bearing heterocyclic capping groups which have different degrees of hydrogen bonding potential and nitrogen substitution. For these four peptides, zeta potential and electrical impedance spectroscopy measurements were undertaken to monitor gelation, with the impedance data showing different gelation times for each peptide hydrogel. Through a combination of atomic force microscopy and rheological measurmeents, including dynamic strain and frequency sweeps, and thixotropic tests, the relationship between the mechanism of self-assembly in these hydrogels and their macroscopic behaviour can be established. It is observed that the degree of nitrogen substitution affects the self-assembly mechanisms of the hydrogels and as such, that there is an interplay between branching and bundling self-assembly pathways that are responsible for the final properties of each hydrogel.

Transcriptome sequencing reveals high isoform diversity in the ant Formica exsecta

PubMed Central

Paviala, Jenni; Morandin, Claire; Wheat, Christopher; Sundström, Liselotte; Helanterä, Heikki

2017-01-01

Transcriptome resources for social insects have the potential to provide new insight into polyphenism, i.e., how divergent phenotypes arise from the same genome. Here we present a transcriptome based on paired-end RNA sequencing data for the ant Formica exsecta (Formicidae, Hymenoptera). The RNA sequencing libraries were constructed from samples of several life stages of both sexes and female castes of queens and workers, in order to maximize representation of expressed genes. We first compare the performance of common assembly and scaffolding software (Trinity, Velvet-Oases, and SOAPdenovo-trans), in producing de novo assemblies. Second, we annotate the resulting expressed contigs to the currently published genomes of ants, and other insects, including the honeybee, to filter genes that have annotation evidence of being true genes. Our pipeline resulted in a final assembly of altogether 39,262 mRNA transcripts, with an average coverage of >300X, belonging to 17,496 unique genes with annotation in the related ant species. From these genes, 536 genes were unique to one caste or sex only, highlighting the importance of comprehensive sampling. Our final assembly also showed expression of several splice variants in 6,975 genes, and we show that accounting for splice variants affects the outcome of downstream analyses such as gene ontologies. Our transcriptome provides an outstanding resource for future genetic studies on F. exsecta and other ant species, and the presented transcriptome assembly can be adapted to any non-model species that has genomic resources available from a related taxon. PMID:29177112

Composite lamination method

NASA Technical Reports Server (NTRS)

Dickerson, G. E. (Inventor)

1977-01-01

A process was developed for preparing relatively thick composite laminate structure wherein thin layers of prepreg tapes are assembled, these thin layers are cut into strips that are partially cured, and stacked into the desired thickness with uncured prepreg disposed between each layer of strips. The formed laminate is finally cured and thereafter machined to the desired final dimensions.