Component model reduction via the projection and assembly method

NASA Technical Reports Server (NTRS)

Bernard, Douglas E.

1989-01-01

The problem of acquiring a simple but sufficiently accurate model of a dynamic system is made more difficult when the dynamic system of interest is a multibody system comprised of several components. A low order system model may be created by reducing the order of the component models and making use of various available multibody dynamics programs to assemble them into a system model. The difficulty is in choosing the reduced order component models to meet system level requirements. The projection and assembly method, proposed originally by Eke, solves this difficulty by forming the full order system model, performing model reduction at the the system level using system level requirements, and then projecting the desired modes onto the components for component level model reduction. The projection and assembly method is analyzed to show the conditions under which the desired modes are captured exactly; to the numerical precision of the algorithm.

Pilot Field Demonstration of Alternative Fuels in Force Projection Petroleum and Water Distribution Equipment

DTIC Science & Technology

2014-09-04

They included two Force Projection Technology (FPT) diesel driven pumping assemblies of 350 and 600 gallons per minute (GPM), and the Advanced...Army Tank Automotive Research Development and Engineering Center (TARDEC). They included two Force Projection Technology (FPT) diesel driven...research programs. The first two systems identified were Force Projection Technology (FPT) diesel -driven pumping assemblies of 350 and 600 gallons per

Splash bar for cooling tower fill assembly

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stackhouse, D.W.; Heidl, S.C.

1987-11-10

A crossflow cooling tower fill assembly for allowing liquid to fall down through the fill assembly and for allowing cooling air to flow through the fill assembly transverse to the flow of the liquid in order to cool the liquid is described. The assembly comprises: longitudinal splash bars; and means for supporting the splash bars so that the splash bars are substantially horizontal and parallel to one another and arranged in vertically spaced, substantially horizontal planes. The splash bars in each plane are horizontally spaced from one another to allow the liquid to fall down between the splash bars tomore » the planes of splash bars below. Each splash bar includes a substantially horizontal, longitudinally extending top web member having (1) longitudinally extending, downwardly projecting vertical side web members, both of the side web members having a lower longitudinal edge with a longitudinally extending, inwardly projecting flange, and (2) at least one longitudinally extending, downardly projecting rib web member between the side web members. Each rib web member has a lower longitudinal edge with a longitudinally extending, laterally projecting flange.« less

Alignment of 1000 Genomes Project reads to reference assembly GRCh38.

PubMed

Zheng-Bradley, Xiangqun; Streeter, Ian; Fairley, Susan; Richardson, David; Clarke, Laura; Flicek, Paul

2017-07-01

The 1000 Genomes Project produced more than 100 trillion basepairs of short read sequence from more than 2600 samples in 26 populations over a period of five years. In its final phase, the project released over 85 million genotyped and phased variants on human reference genome assembly GRCh37. An updated reference assembly, GRCh38, was released in late 2013, but there was insufficient time for the final phase of the project analysis to change to the new assembly. Although it is possible to lift the coordinates of the 1000 Genomes Project variants to the new assembly, this is a potentially error-prone process as coordinate remapping is most appropriate only for non-repetitive regions of the genome and those that did not see significant change between the two assemblies. It will also miss variants in any region that was newly added to GRCh38. Thus, to produce the highest quality variants and genotypes on GRCh38, the best strategy is to realign the reads and recall the variants based on the new alignment. As the first step of variant calling for the 1000 Genomes Project data, we have finished remapping all of the 1000 Genomes sequence reads to GRCh38 with alternative scaffold-aware BWA-MEM. The resulting alignments are available as CRAM, a reference-based sequence compression format. The data have been released on our FTP site and are also available from European Nucleotide Archive to facilitate researchers discovering variants on the primary sequences and alternative contigs of GRCh38. © The Authors 2017. Published by Oxford University Press.

Development of cable drive systems for an automated assembly project

NASA Technical Reports Server (NTRS)

Monroe, Charles A., Jr.

1990-01-01

In a robotic assembly project, a method was needed to accurately position a robot and a structure which the robot was to assemble. The requirements for high precision and relatively long travel distances dictated the use of cable drive systems. The design of the mechanisms used in translating the robot and in rotating the assembly under construction is discussed. The design criteria are discussed, and the effect of particular requirements on the design is noted. Finally, the measured performance of the completed mechanism is compared with design requirements.

Advanced Stirling Radioisotope Generator Engineering Unit 2 (ASRG EU2) Final Assembly

NASA Technical Reports Server (NTRS)

Oriti, Salvatore M.

2015-01-01

NASA Glenn Research Center (GRC) has recently completed the assembly of a unique Stirling generator test article for laboratory experimentation. Under the Advanced Stirling Radioisotope Generator (ASRG) flight development contract, NASA GRC initiated a task to design and fabricate a flight-like generator for in-house testing. This test article was given the name ASRG Engineering Unit 2 (EU2) as it was effectively the second engineering unit to be built within the ASRG project. The intent of the test article was to duplicate Lockheed Martin's qualification unit ASRG design as much as possible to enable system-level tests not previously possible at GRC. After the cancellation of the ASRG flight development project, the decision was made to continue the EU2 build, and make use of a portion of the hardware from the flight development project. GRC and Lockheed Martin engineers collaborated to develop assembly procedures, leveraging the valuable knowledge gathered by Lockheed Martin during the ASRG development contract. The ASRG EU2 was then assembled per these procedures at GRC with Lockheed Martin engineers on site. The assembly was completed in August 2014. This paper details the components that were used for the assembly, and the assembly process itself.

Gap Resolution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Labutti, Kurt; Foster, Brian; Lapidus, Alla

Gap Resolution is a software package that was developed to improve Newbler genome assemblies by automating the closure of sequence gaps caused by repetitive regions in the DNA. This is done by performing the follow steps:1) Identify and distribute the data for each gap in sub-projects. 2) Assemble the data associated with each sub-project using a secondary assembler, such as Newbler or PGA. 3) Determine if any gaps are closed after reassembly, and either design fakes (consensus of closed gap) for those that closed or lab experiments for those that require additional data. The software requires as input a genomemore » assembly produce by the Newbler assembler provided by Roche and 454 data containing paired-end reads.« less

Experimental Demonstration of Technologies for Autonomous On-Orbit Robotic Assembly

NASA Technical Reports Server (NTRS)

LeMaster, Edward A.; Schaechter, David B.; Carrington, Connie K.

2006-01-01

The Modular Reconfigurable High Energy (MRHE) program aimed to develop technologies for the automated assembly and deployment of large-scale space structures and aggregate spacecraft. Part of the project involved creation of a terrestrial robotic testbed for validation and demonstration of these technologies and for the support of future development activities. This testbed was completed in 2005, and was thereafter used to demonstrate automated rendezvous, docking, and self-assembly tasks between a group of three modular robotic spacecraft emulators. This paper discusses the rationale for the MRHE project, describes the testbed capabilities, and presents the MRHE assembly demonstration sequence.

Circuit breaker lock out assembly

DOEpatents

Gordy, W.T.

1983-05-18

A lock out assembly for a circuit breaker which consists of a generally step-shaped unitary base with an aperture in the small portion of the step-shaped base and a roughly S shaped retaining pin which loops through the large portion of the step-shaped base. The lock out assembly is adapted to fit over a circuit breaker with the handle switch projecting through the aperture, and the retaining pin projecting into an opening of the handle switch, preventing removal.

Circuit breaker lock out assembly

DOEpatents

Gordy, Wade T.

1984-01-01

A lock out assembly for a circuit breaker which consists of a generally step-shaped unitary base with an aperture in the small portion of the step-shaped base and a roughly "S" shaped retaining pin which loops through the large portion of the step-shaped base. The lock out assembly is adapted to fit over a circuit breaker with the handle switch projecting through the aperture, and the retaining pin projecting into an opening of the handle switch, preventing removal.

Program to Develop High Strength Aluminum Powder Metallurgy Mill Products - Phase IV-B-Scale - up to 3200 lb Billet

DTIC Science & Technology

1977-04-25

H . Dudas and J . Paul Lyle, Jr. as project supervisors. The Frankford Arsenal Project Engineer...container was controlled to minimize dust emissions through any of the seals in the complete assembly. A gas bypass line allowed the displaced gas in...2) the cylinder, a shrink fit assembly of an H -12 steel liner and two outer retaining rings; and (3) a ram assembly consisting of a ram nose,

Progress toward a low budget reference grade genome assembly

USDA-ARS?s Scientific Manuscript database

Reference quality de novo genome assemblies were once solely the domain of large, well-funded genome projects. While next-generation short read technology removed some of the cost barriers, accurate chromosome-scale assembly remains a real challenge. Here we present efforts to de novo assemble the...

preAssemble: a tool for automatic sequencer trace data processing.

PubMed

Adzhubei, Alexei A; Laerdahl, Jon K; Vlasova, Anna V

2006-01-17

Trace or chromatogram files (raw data) are produced by automatic nucleic acid sequencing equipment or sequencers. Each file contains information which can be interpreted by specialised software to reveal the sequence (base calling). This is done by the sequencer proprietary software or publicly available programs. Depending on the size of a sequencing project the number of trace files can vary from just a few to thousands of files. Sequencing quality assessment on various criteria is important at the stage preceding clustering and contig assembly. Two major publicly available packages--Phred and Staden are used by preAssemble to perform sequence quality processing. The preAssemble pre-assembly sequence processing pipeline has been developed for small to large scale automatic processing of DNA sequencer chromatogram (trace) data. The Staden Package Pregap4 module and base-calling program Phred are utilized in the pipeline, which produces detailed and self-explanatory output that can be displayed with a web browser. preAssemble can be used successfully with very little previous experience, however options for parameter tuning are provided for advanced users. preAssemble runs under UNIX and LINUX operating systems. It is available for downloading and will run as stand-alone software. It can also be accessed on the Norwegian Salmon Genome Project web site where preAssemble jobs can be run on the project server. preAssemble is a tool allowing to perform quality assessment of sequences generated by automatic sequencing equipment. preAssemble is flexible since both interactive jobs on the preAssemble server and the stand alone downloadable version are available. Virtually no previous experience is necessary to run a default preAssemble job, on the other hand options for parameter tuning are provided. Consequently preAssemble can be used as efficiently for just several trace files as for large scale sequence processing.

Cell Assembly Dynamics of Sparsely-Connected Inhibitory Networks: A Simple Model for the Collective Activity of Striatal Projection Neurons.

PubMed

Angulo-Garcia, David; Berke, Joshua D; Torcini, Alessandro

2016-02-01

Striatal projection neurons form a sparsely-connected inhibitory network, and this arrangement may be essential for the appropriate temporal organization of behavior. Here we show that a simplified, sparse inhibitory network of Leaky-Integrate-and-Fire neurons can reproduce some key features of striatal population activity, as observed in brain slices. In particular we develop a new metric to determine the conditions under which sparse inhibitory networks form anti-correlated cell assemblies with time-varying activity of individual cells. We find that under these conditions the network displays an input-specific sequence of cell assembly switching, that effectively discriminates similar inputs. Our results support the proposal that GABAergic connections between striatal projection neurons allow stimulus-selective, temporally-extended sequential activation of cell assemblies. Furthermore, we help to show how altered intrastriatal GABAergic signaling may produce aberrant network-level information processing in disorders such as Parkinson's and Huntington's diseases.

Instrumentation to Aid in Steel Bridge Fabrication : Bridge Virtual Assembly System

DOT National Transportation Integrated Search

2018-05-01

This pool funded project developed a BRIDGE VIRTUAL ASSEMBLY SYSTEM (BRIDGE VAS) that improves manufacturing processes and enhances quality control for steel bridge fabrication. The system replaces conventional match-drilling with virtual assembly me...

in silico Whole Genome Sequencer & Analyzer (iWGS): A Computational Pipeline to Guide the Design and Analysis of de novo Genome Sequencing Studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Xiaofan; Peris, David; Kominek, Jacek

The availability of genomes across the tree of life is highly biased toward vertebrates, pathogens, human disease models, and organisms with relatively small and simple genomes. Recent progress in genomics has enabled the de novo decoding of the genome of virtually any organism, greatly expanding its potential for understanding the biology and evolution of the full spectrum of biodiversity. The increasing diversity of sequencing technologies, assays, and de novo assembly algorithms have augmented the complexity of de novo genome sequencing projects in nonmodel organisms. To reduce the costs and challenges in de novo genome sequencing projects and streamline their experimentalmore » design and analysis, we developed iWGS (in silico Whole Genome Sequencer and Analyzer), an automated pipeline for guiding the choice of appropriate sequencing strategy and assembly protocols. iWGS seamlessly integrates the four key steps of a de novo genome sequencing project: data generation (through simulation), data quality control, de novo assembly, and assembly evaluation and validation. The last three steps can also be applied to the analysis of real data. iWGS is designed to enable the user to have great flexibility in testing the range of experimental designs available for genome sequencing projects, and supports all major sequencing technologies and popular assembly tools. Three case studies illustrate how iWGS can guide the design of de novo genome sequencing projects, and evaluate the performance of a wide variety of user-specified sequencing strategies and assembly protocols on genomes of differing architectures. iWGS, along with a detailed documentation, is freely available at https://github.com/zhouxiaofan1983/iWGS.« less

in silico Whole Genome Sequencer & Analyzer (iWGS): A Computational Pipeline to Guide the Design and Analysis of de novo Genome Sequencing Studies

DOE PAGES

Zhou, Xiaofan; Peris, David; Kominek, Jacek; ...

2016-09-16

The availability of genomes across the tree of life is highly biased toward vertebrates, pathogens, human disease models, and organisms with relatively small and simple genomes. Recent progress in genomics has enabled the de novo decoding of the genome of virtually any organism, greatly expanding its potential for understanding the biology and evolution of the full spectrum of biodiversity. The increasing diversity of sequencing technologies, assays, and de novo assembly algorithms have augmented the complexity of de novo genome sequencing projects in nonmodel organisms. To reduce the costs and challenges in de novo genome sequencing projects and streamline their experimentalmore » design and analysis, we developed iWGS (in silico Whole Genome Sequencer and Analyzer), an automated pipeline for guiding the choice of appropriate sequencing strategy and assembly protocols. iWGS seamlessly integrates the four key steps of a de novo genome sequencing project: data generation (through simulation), data quality control, de novo assembly, and assembly evaluation and validation. The last three steps can also be applied to the analysis of real data. iWGS is designed to enable the user to have great flexibility in testing the range of experimental designs available for genome sequencing projects, and supports all major sequencing technologies and popular assembly tools. Three case studies illustrate how iWGS can guide the design of de novo genome sequencing projects, and evaluate the performance of a wide variety of user-specified sequencing strategies and assembly protocols on genomes of differing architectures. iWGS, along with a detailed documentation, is freely available at https://github.com/zhouxiaofan1983/iWGS.« less

High power s-band vacuum load

DOE Office of Scientific and Technical Information (OSTI.GOV)

Neubauer, Michael; Dudas, Alan; Krasnykh, Anatoly

Through a combination of experimentation and calculation the components of a novel room temperature dry load were successfully fabricated. These components included lossy ceramic cylinders of various lengths, thicknesses, and percent of silicon carbide (SiC). The cylinders were then assembled into stainless steel compression rings by differential heating of the parts and a special fixture. Post machining of this assembly provided a means for a final weld. The ring assemblies were then measured for S-parameters, individually and in pairs using a low-cost TE10 rectangular to TE01 circular waveguide adapter specially designed to be part of the final load assembly. Matchedmore » pairs of rings were measured for assembly into the final load, and a sliding short designed and fabricated to assist in determining the desired short location in the final assembly. The plan for the project was for Muons, Inc. to produce prototype loads for long-term testing at SLAC. The STTR funds for SLAC were to upgrade and operate their test station to ensure that the loads would satisfy their requirements. Phase III was to be the sale to SLAC of loads that Muons, Inc. would manufacture. However, an alternate solution that involved a rebuild of the old loads, reduced SLAC budget projections, and a relaxed time for the replacement of all loads meant that in-house labor will be used to do the upgrade without the need for the loads developed in this project. Consequently, the project was terminated before the long term testing was initiated. However, SLAC can use the upgraded test stand to compare the long-term performance of the ones produced in this project with their rebuilt loads when they are available.« less

NASA-DoD Lead-Free Electronics Project

NASA Technical Reports Server (NTRS)

Kessel, Kurt

2007-01-01

The primary technical objective of the project is to undertake comprehensive testing to generate information on failure modes/criteria to better understand the reliability of: Packages (e.g., TSOP, BGA, PDIP) assembled and reworked with lead-free alloys Packages (e.g., TSOP, BGA, PDIP) assembled and reworked with mixed (lead/lead-free) alloys.

An efficient approach to BAC based assembly of complex genomes.

PubMed

Visendi, Paul; Berkman, Paul J; Hayashi, Satomi; Golicz, Agnieszka A; Bayer, Philipp E; Ruperao, Pradeep; Hurgobin, Bhavna; Montenegro, Juan; Chan, Chon-Kit Kenneth; Staňková, Helena; Batley, Jacqueline; Šimková, Hana; Doležel, Jaroslav; Edwards, David

2016-01-01

There has been an exponential growth in the number of genome sequencing projects since the introduction of next generation DNA sequencing technologies. Genome projects have increasingly involved assembly of whole genome data which produces inferior assemblies compared to traditional Sanger sequencing of genomic fragments cloned into bacterial artificial chromosomes (BACs). While whole genome shotgun sequencing using next generation sequencing (NGS) is relatively fast and inexpensive, this method is extremely challenging for highly complex genomes, where polyploidy or high repeat content confounds accurate assembly, or where a highly accurate 'gold' reference is required. Several attempts have been made to improve genome sequencing approaches by incorporating NGS methods, to variable success. We present the application of a novel BAC sequencing approach which combines indexed pools of BACs, Illumina paired read sequencing, a sequence assembler specifically designed for complex BAC assembly, and a custom bioinformatics pipeline. We demonstrate this method by sequencing and assembling BAC cloned fragments from bread wheat and sugarcane genomes. We demonstrate that our assembly approach is accurate, robust, cost effective and scalable, with applications for complete genome sequencing in large and complex genomes.

Nondestructive Assay Data Integration with the SKB-50 Assemblies - FY16 Update

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tobin, Stephen Joseph; Fugate, Michael Lynn; Trellue, Holly Renee

2016-10-28

A project to research the application of non-destructive assay (NDA) techniques for spent fuel assemblies is underway at the Central Interim Storage Facility for Spent Nuclear Fuel (for which the Swedish acronym is Clab) in Oskarshamn, Sweden. The research goals of this project contain both safeguards and non-safeguards interests. These nondestructive assay (NDA) technologies are designed to strengthen the technical toolkit of safeguard inspectors and others to determine the following technical goals more accurately; Verify initial enrichment, burnup, and cooling time of facility declaration for spent fuel assemblies; Detect replaced or missing pins from a given spent fuel assembly tomore » confirm its integrity; and Estimate plutonium mass and related plutonium and uranium fissile mass parameters in spent fuel assemblies. Estimate heat content, and measure reactivity (multiplication).« less

Regeneration and tritium recovery from the large JET neutral injection cryopump system after the FTE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Obert, W.; Bell, A.; Davies, J.

1992-12-01

Neutral Beam Injection (NBI) was used to introduce tritium into the plasma for the First Tritium Experiment In addition to the decisive advantage of depositing the tritium into the centre of the plasma, the use of NBI also minimized the total quantity of tritium introduced into the Torus and the contamination of the vacuum vessel. However, because of the relatively low gas efficiency of the positive ion injection system approximately 95% of the total quantity of tritium introduced was pumped by the large condensation cryopumps which form an integral part of the injector. Several hardware and associated software changes weremore » implemented in order to making provision for possible fault scenarios during operation with tritium and to ensure complete regeneration of the tritium from the cryopumps. The tritium released after all subsequent regeneration`s has been monitored carefully in order to determine the amount of tritium retained by the black anodized liquid nitrogen panel surfaces of the cryopump and to compare it with experiments at TSTA on JET samples before the FTE.« less

Regeneration and tritium recovery from the large JET neutral injection cryopump system after the FTE. [First Tritium Experiment (FTE)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Obert, W.; Bell, A.; Davies, J.

1992-01-01

Neutral Beam Injection (NBI) was used to introduce tritium into the plasma for the First Tritium Experiment In addition to the decisive advantage of depositing the tritium into the centre of the plasma, the use of NBI also minimized the total quantity of tritium introduced into the Torus and the contamination of the vacuum vessel. However, because of the relatively low gas efficiency of the positive ion injection system approximately 95% of the total quantity of tritium introduced was pumped by the large condensation cryopumps which form an integral part of the injector. Several hardware and associated software changes weremore » implemented in order to making provision for possible fault scenarios during operation with tritium and to ensure complete regeneration of the tritium from the cryopumps. The tritium released after all subsequent regeneration's has been monitored carefully in order to determine the amount of tritium retained by the black anodized liquid nitrogen panel surfaces of the cryopump and to compare it with experiments at TSTA on JET samples before the FTE.« less

An improved filtering algorithm for big read datasets and its application to single-cell assembly.

PubMed

Wedemeyer, Axel; Kliemann, Lasse; Srivastav, Anand; Schielke, Christian; Reusch, Thorsten B; Rosenstiel, Philip

2017-07-03

For single-cell or metagenomic sequencing projects, it is necessary to sequence with a very high mean coverage in order to make sure that all parts of the sample DNA get covered by the reads produced. This leads to huge datasets with lots of redundant data. A filtering of this data prior to assembly is advisable. Brown et al. (2012) presented the algorithm Diginorm for this purpose, which filters reads based on the abundance of their k-mers. We present Bignorm, a faster and quality-conscious read filtering algorithm. An important new algorithmic feature is the use of phred quality scores together with a detailed analysis of the k-mer counts to decide which reads to keep. We qualify and recommend parameters for our new read filtering algorithm. Guided by these parameters, we remove in terms of median 97.15% of the reads while keeping the mean phred score of the filtered dataset high. Using the SDAdes assembler, we produce assemblies of high quality from these filtered datasets in a fraction of the time needed for an assembly from the datasets filtered with Diginorm. We conclude that read filtering is a practical and efficient method for reducing read data and for speeding up the assembly process. This applies not only for single cell assembly, as shown in this paper, but also to other projects with high mean coverage datasets like metagenomic sequencing projects. Our Bignorm algorithm allows assemblies of competitive quality in comparison to Diginorm, while being much faster. Bignorm is available for download at https://git.informatik.uni-kiel.de/axw/Bignorm .

Telemetry Simulation Assembly Implementation in the DSN

NASA Technical Reports Server (NTRS)

Alberda, M. E.

1984-01-01

The telemetry simulation was implemented as part of the MARK IV network implementation project. The telemetry simulation assembly (TSA) is replacing the Simulation Conversion Assembly (SCA) throughout the DSN. The development of the TSA is discussed, and the design is described to the block diagram level.

Projection optics box

DOEpatents

Hale, Layton C.; Malsbury, Terry; Hudyma, Russell M.; Parker, John M.

2000-01-01

A projection optics box or assembly for use in an optical assembly, such as in an extreme ultraviolet lithography (EUVL) system using 10-14 nm soft x-ray photons. The projection optics box utilizes a plurality of highly reflective optics or mirrors, each mounted on a precision actuator, and which reflects an optical image, such as from a mask, in the EUVL system onto a point of use, such as a target or silicon wafer, the mask, for example, receiving an optical signal from a source assembly, such as a developed from laser system, via a series of highly reflective mirrors of the EUVL system. The plurality of highly reflective optics or mirrors are mounted in a housing assembly comprised of a series of bulkheads having wall members secured together to form a unit construction of maximum rigidity. Due to the precision actuators, the mirrors must be positioned precisely and remotely in tip, tilt, and piston (three degrees of freedom), while also providing exact constraint.

NASA DOD Lead Free Electronics Project

NASA Technical Reports Server (NTRS)

Kessel, Kurt R.

2008-01-01

The primary'technical objective of this project is to undertake comprehensive testing to generate information on failure modes/criteria to better understand the reliability of: Packages (e.g., Thin Small Outline Package [TSOP], Ball Grid Array [BGA], Plastic Dual In-line Package [PDIPD assembled and reworked with lead-free alloys Packages (e.g., TSOP, BGA, PDIP) assembled and reworked with mixed (lead/lead-free) alloys.

BACCardI--a tool for the validation of genomic assemblies, assisting genome finishing and intergenome comparison.

PubMed

Bartels, Daniela; Kespohl, Sebastian; Albaum, Stefan; Drüke, Tanja; Goesmann, Alexander; Herold, Julia; Kaiser, Olaf; Pühler, Alfred; Pfeiffer, Friedhelm; Raddatz, Günter; Stoye, Jens; Meyer, Folker; Schuster, Stephan C

2005-04-01

We provide the graphical tool BACCardI for the construction of virtual clone maps from standard assembler output files or BLAST based sequence comparisons. This new tool has been applied to numerous genome projects to solve various problems including (a) validation of whole genome shotgun assemblies, (b) support for contig ordering in the finishing phase of a genome project, and (c) intergenome comparison between related strains when only one of the strains has been sequenced and a large insert library is available for the other. The BACCardI software can seamlessly interact with various sequence assembly packages. Genomic assemblies generated from sequence information need to be validated by independent methods such as physical maps. The time-consuming task of building physical maps can be circumvented by virtual clone maps derived from read pair information of large insert libraries.

Construction and assembly of the wire planes for the MicroBooNE Time Projection Chamber

DOE Office of Scientific and Technical Information (OSTI.GOV)

Acciarri, R.; Adams, C.; Asaadi, J.

In this paper we describe how the readout planes for the MicroBooNE Time Projection Chamber were constructed, assembled and installed. We present the individual wire preparation using semi-automatic winding machines and the assembly of wire carrier boards. The details of the wire installation on the detector frame and the tensioning of the wires are given. A strict quality assurance plan ensured the integrity of the readout planes. The different tests performed at all stages of construction and installation provided crucial information to achieve the successful realization of the MicroBooNE wire planes.

Comparing de novo assemblers for 454 transcriptome data

PubMed Central

2010-01-01

Background Roche 454 pyrosequencing has become a method of choice for generating transcriptome data from non-model organisms. Once the tens to hundreds of thousands of short (250-450 base) reads have been produced, it is important to correctly assemble these to estimate the sequence of all the transcripts. Most transcriptome assembly projects use only one program for assembling 454 pyrosequencing reads, but there is no evidence that the programs used to date are optimal. We have carried out a systematic comparison of five assemblers (CAP3, MIRA, Newbler, SeqMan and CLC) to establish best practices for transcriptome assemblies, using a new dataset from the parasitic nematode Litomosoides sigmodontis. Results Although no single assembler performed best on all our criteria, Newbler 2.5 gave longer contigs, better alignments to some reference sequences, and was fast and easy to use. SeqMan assemblies performed best on the criterion of recapitulating known transcripts, and had more novel sequence than the other assemblers, but generated an excess of small, redundant contigs. The remaining assemblers all performed almost as well, with the exception of Newbler 2.3 (the version currently used by most assembly projects), which generated assemblies that had significantly lower total length. As different assemblers use different underlying algorithms to generate contigs, we also explored merging of assemblies and found that the merged datasets not only aligned better to reference sequences than individual assemblies, but were also more consistent in the number and size of contigs. Conclusions Transcriptome assemblies are smaller than genome assemblies and thus should be more computationally tractable, but are often harder because individual contigs can have highly variable read coverage. Comparing single assemblers, Newbler 2.5 performed best on our trial data set, but other assemblers were closely comparable. Combining differently optimal assemblies from different programs however gave a more credible final product, and this strategy is recommended. PMID:20950480

Skylab communications carrier 16536G and filter bypass adapter assembly 12535G. [development of communications equipment for use with Skylab spacecraft

NASA Technical Reports Server (NTRS)

1974-01-01

Communications equipment for use with the Skylab project is examined to show compliance with contract requirements. The items of equipment considered are: (1) communications carrier assemblies, (2) filter bypass adapter assemblies, and (3) sub-assemblies, parts, and repairs. Additional information is provided concerning contract requirements, test requirements, and failure investigation actions.

NASA-DoD Lead-Free Electronics Project

NASA Technical Reports Server (NTRS)

Kessel, Kurt R.

2009-01-01

The primary technical objective of this project is to undertake comprehensive testing to generate information on failure modes/criteria to better understand the reliability of: (1) Packages (e.g., Thin Small Outline Package [TSOP], Ball Grid Array [BGA], Plastic Dual In-line Package [PDIP]) assembled and reworked with lead-free alloys, (2) Packages (e.g., TSOP, BGA, PDIP) assembled and reworked with mixed (lead/lead-free) alloys.

Engineering at SLAC: Designing and constructing experimental devices for the Stanford Synchrotron Radiation Lightsource - Final Paper

DOE Office of Scientific and Technical Information (OSTI.GOV)

Djang, Austin

2015-08-22

Thanks to the versatility of the beam lines at SSRL, research there is varied and benefits multiple fields. Each experiment requires a particular set of experiment equipment, which in turns requires its own particular assembly. As such, new engineering challenges arise from each new experiment. My role as an engineering intern has been to help solve these challenges, by designing and assembling experimental devices. My first project was to design a heated sample holder, which will be used to investigate the effect of temperature on a sample's x-ray diffraction pattern. My second project was to help set up an imagingmore » test, which involved designing a cooled grating holder and assembling multiple positioning stages. My third project was designing a 3D-printed pencil holder for the SSRL workstations.« less

Seamless tiled display system

NASA Technical Reports Server (NTRS)

Dubin, Matthew B. (Inventor); Larson, Brent D. (Inventor); Kolosowsky, Aleksandra (Inventor)

2006-01-01

A modular and scalable seamless tiled display apparatus includes multiple display devices, a screen, and multiple lens assemblies. Each display device is subdivided into multiple sections, and each section is configured to display a sectional image. One of the lens assemblies is optically coupled to each of the sections of each of the display devices to project the sectional image displayed on that section onto the screen. The multiple lens assemblies are configured to merge the projected sectional images to form a single tiled image. The projected sectional images may be merged on the screen by magnifying and shifting the images in an appropriate manner. The magnification and shifting of these images eliminates any visual effect on the tiled display that may result from dead-band regions defined between each pair of adjacent sections on each display device, and due to gaps between multiple display devices.

Genome Improvement at JGI-HAGSC

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grimwood, Jane; Schmutz, Jeremy J.; Myers, Richard M.

Since the completion of the sequencing of the human genome, the Joint Genome Institute (JGI) has rapidly expanded its scientific goals in several DOE mission-relevant areas. At the JGI-HAGSC, we have kept pace with this rapid expansion of projects with our focus on assessing, assembling, improving and finishing eukaryotic whole genome shotgun (WGS) projects for which the shotgun sequence is generated at the Production Genomic Facility (JGI-PGF). We follow this by combining the draft WGS with genomic resources generated at JGI-HAGSC or in collaborator laboratories (including BAC end sequences, genetic maps and FLcDNA sequences) to produce an improved draft sequence.more » For eukaryotic genomes important to the DOE mission, we then add further information from directed experiments to produce reference genomic sequences that are publicly available for any scientific researcher. Also, we have continued our program for producing BAC-based finished sequence, both for adding information to JGI genome projects and for small BAC-based sequencing projects proposed through any of the JGI sequencing programs. We have now built our computational expertise in WGS assembly and analysis and have moved eukaryotic genome assembly from the JGI-PGF to JGI-HAGSC. We have concentrated our assembly development work on large plant genomes and complex fungal and algal genomes.« less

Short-term memory in olfactory network dynamics

NASA Astrophysics Data System (ADS)

Stopfer, Mark; Laurent, Gilles

1999-12-01

Neural assemblies in a number of animal species display self-organized, synchronized oscillations in response to sensory stimuli in a variety of brain areas.. In the olfactory system of insects, odour-evoked oscillatory synchronization of antennal lobe projection neurons (PNs) is superimposed on slower and stimulus-specific temporal activity patterns. Hence, each odour activates a specific and dynamic projection neuron assembly whose evolution during a stimulus is locked to the oscillation clock. Here we examine, using locusts, the changes in population dynamics of projection-neuron assemblies over repeated odour stimulations, as would occur when an animal first encounters and then repeatedly samples an odour for identification or localization. We find that the responses of these assemblies rapidly decrease in intensity, while they show a marked increase in spike time precision and inter-neuronal oscillatory coherence. Once established, this enhanced precision in the representation endures for several minutes. This change is stimulus-specific, and depends on events within the antennal lobe circuits, independent of olfactory receptor adaptation: it may thus constitute a form of sensory memory. Our results suggest that this progressive change in olfactory network dynamics serves to converge, over repeated odour samplings, on a more precise and readily classifiable odour representation, using relational information contained across neural assemblies.

Research to Assembly Scheme for Satellite Deck Based on Robot Flexibility Control Principle

NASA Astrophysics Data System (ADS)

Guo, Tao; Hu, Ruiqin; Xiao, Zhengyi; Zhao, Jingjing; Fang, Zhikai

2018-03-01

Deck assembly is critical quality control point in final satellite assembly process, and cable extrusion and structure collision problems in assembly process will affect development quality and progress of satellite directly. Aimed at problems existing in deck assembly process, assembly project scheme for satellite deck based on robot flexibility control principle is proposed in this paper. Scheme is introduced firstly; secondly, key technologies on end force perception and flexible docking control in the scheme are studied; then, implementation process of assembly scheme for satellite deck is described in detail; finally, actual application case of assembly scheme is given. Result shows that compared with traditional assembly scheme, assembly scheme for satellite deck based on robot flexibility control principle has obvious advantages in work efficiency, reliability and universality aspects etc.

Flat-plate solar array project. Volume 5: Process development

NASA Technical Reports Server (NTRS)

Gallagher, B.; Alexander, P.; Burger, D.

1986-01-01

The goal of the Process Development Area, as part of the Flat-Plate Solar Array (FSA) Project, was to develop and demonstrate solar cell fabrication and module assembly process technologies required to meet the cost, lifetime, production capacity, and performance goals of the FSA Project. R&D efforts expended by Government, Industry, and Universities in developing processes capable of meeting the projects goals during volume production conditions are summarized. The cost goals allocated for processing were demonstrated by small volume quantities that were extrapolated by cost analysis to large volume production. To provide proper focus and coverage of the process development effort, four separate technology sections are discussed: surface preparation, junction formation, metallization, and module assembly.

A post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs.

PubMed

Swain, Martin T; Tsai, Isheng J; Assefa, Samual A; Newbold, Chris; Berriman, Matthew; Otto, Thomas D

2012-06-07

Genome projects now produce draft assemblies within weeks owing to advanced high-throughput sequencing technologies. For milestone projects such as Escherichia coli or Homo sapiens, teams of scientists were employed to manually curate and finish these genomes to a high standard. Nowadays, this is not feasible for most projects, and the quality of genomes is generally of a much lower standard. This protocol describes software (PAGIT) that is used to improve the quality of draft genomes. It offers flexible functionality to close gaps in scaffolds, correct base errors in the consensus sequence and exploit reference genomes (if available) in order to improve scaffolding and generating annotations. The protocol is most accessible for bacterial and small eukaryotic genomes (up to 300 Mb), such as pathogenic bacteria, malaria and parasitic worms. Applying PAGIT to an E. coli assembly takes ∼24 h: it doubles the average contig size and annotates over 4,300 gene models.

Differential Die-Away Instrument: Report on Fuel Assembly Mock-up Measurements with Neutron Generator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goodsell, Alison Victoria; Swinhoe, Martyn Thomas; Henzl, Vladimir

2014-09-18

Fresh fuel experiments for the differential die-away (DDA) project were performed using a DT neutron generator, a 15x15 PWR fuel assembly, and nine 3He detectors in a water tank inside of a shielded cell at Los Alamos National Laboratory (LANL). Eight different fuel enrichments were created using low enriched (LEU) and depleted uranium (DU) dioxide fuel rods. A list-mode data acquisition system recorded the time-dependent signal and analysis of the DDA signal die-away time was performed. The die-away time depended on the amount of fissile material in the fuel assembly and the position of the detector. These experiments were performedmore » in support of the spent nuclear fuel Next Generation Safeguards Initiative DDA project. Lessons learned from the fresh fuel DDA instrument experiments and simulations will provide useful information to the spent fuel project.« less

NASA-DoD Lead-Free Electronics Project

NASA Technical Reports Server (NTRS)

Kessel, Kurt R.

2009-01-01

In response to concerns about risks from lead-free induced faults to high reliability products, NASA has initiated a multi-year project to provide manufacturers and users with data to clarify the risks of lead-free materials in their products. The project will also be of interest to component manufacturers supplying to high reliability markets. The project was launched in November 2006. The primary technical objective of the project is to undertake comprehensive testing to generate information on failure modes/criteria to better understand the reliability of: - Packages (e.g., TSOP, BOA, PDIP) assembled and reworked with solder interconnects consisting of lead-free alloys - Packages (e.g., TSOP, BOA, PDIP) assembled and reworked with solder interconnects consisting of mixed alloys, lead component finish/lead-free solder and lead-free component finish/SnPb solder.

NASA-DoD Lead-Free Electronics Project

NASA Technical Reports Server (NTRS)

Kessel, Kurt R.

2009-01-01

In response to concerns about risks from lead-free induced faults to high reliability products, NASA has initiated a multi-year project to provide manufacturers and users with data to clarify the risks of lead-free materials in their products. The project will also be of interest to component manufacturers supplying to high reliability markets. The project was launched in November 2006. The primary technical objective of the project is to undertake comprehensive testing to generate information on failure modes/criteria to better understand the reliability of: - Packages (e.g., TSOP, BGA, PDIP) assembled and reworked with solder interconnects consisting of lead-free alloys - Packages (e.g., TSOP, BGA, PDIP) assembled and reworked with solder interconnects consisting of mixed alloys, lead component finish/lead-free solder and lead-free component finish/SnPb solder.

CFRP composite optical telescope assembly for the 1 m ULTRA project

NASA Astrophysics Data System (ADS)

Martin, Robert N.; Romeo, Robert C.

2006-06-01

The focus of the ULTRA Project is to develop and test Ultra-Lightweight Technology for Research applications in Astronomy. The ULTRA project is a collaborative effort involving the private firm Composite Mirror Applications, Inc (CMA) and 3 universities: University of Kansas, San Diego State University, and Dartmouth College. Funding for ULTRA is predominately from a NSF three year MRI program grant to CMA and KU with additional support from CMA, KU and SDSU. The goal of the ULTRA program is to demonstrate that a viable alternative exists to traditional glass mirror and steel telescope technology by designing, fabricating and testing a research telescope constructed from carbon fiber reinforced plastic (CFRP) materials. In particular, a 1m diameter, Cassegrain telescope optics set and optical tube assembly (OTA) are being designed and fabricated by CMA. The completed telescope will be deployed at SDSU's Mt Laguna Observatory in a refurbished structure (new dome and mount provided via KU and SDSU). We expect that a successful completion and testing of this project will lead to future use of CFRP technology in larger telescopes and segmented telescopes. This paper describes the OTA (optical tube assembly) that has been developed for the ULTRA project. The mirror technology is described in another paper in this conference. A poster describes the ULTRA project overview in more detail.

Algorithm of choosing type of mechanical assembly production of instrument making enterprises of Industry 4.0

NASA Astrophysics Data System (ADS)

Zakoldaev, D. A.; Shukalov, A. V.; Zharinov, I. O.; Zharinov, O. O.

2018-05-01

The task of the algorithm of choosing the type of mechanical assembly production of instrument making enterprises of Industry 4.0 is being studied. There is a comparison of two project algorithms for Industry 3.0 and Industry 4.0. The algorithm of choosing the type of mechanical assembly production of instrument making enterprises of Industry 4.0 is based on the technological route analysis of the manufacturing process in a company equipped with cyber and physical systems. This algorithm may give some project solutions selected from the primary part or the auxiliary one of the production. The algorithm decisive rules are based on the optimal criterion.

A probability approach to sawtimber tree-value projections

Treesearch

Roger E. McCay; Paul S. DeBald; Paul S. DeBald

1973-01-01

The authors present a method for projecting hardwood sawtimber tree values, using tree-development probabilities based on continuous forest inventory (CFI) data and describe some ways to use the resulting value projections to assemble management-planning information.

U-238 fission and Pu-239 production in subcritical assembly

NASA Astrophysics Data System (ADS)

Grab, Magdalena; Wojciechowski, Andrzej

2018-04-01

The project touches upon an issue of U-238 fission reactions and Pu-239 production reactions in subcritical assembly. The experiment took place in November 2014 at the Dzhelepov Laboratory of Nuclear Problems (JINR, Dubna) using PHASOTRON.Data of this experiment were analyzed in Laboratory of Information Technologies (LIT). Four MCNPX models were considered for simulation: Bertini/Dresnen, Bertini/Abla, INCL4/Drensnen, INCL4/Abla. The main goal of the project was to compare the experimental data and simulation results. We obtain a good agreement of experimental data and computation results especially for detectors placed besides the assembly axis. In addition, the U-238 fission reactions are more probable to be observed in the region of a higher particle energy spectrum, located closer to the assembly axis and the particle beam as well and vice versa Pu-239 production reactions were dominant in the peripheral region of geometry.

Reference-guided assembly of four diverse Arabidopsis thaliana genomes

PubMed Central

Schneeberger, Korbinian; Ossowski, Stephan; Ott, Felix; Klein, Juliane D.; Wang, Xi; Lanz, Christa; Smith, Lisa M.; Cao, Jun; Fitz, Joffrey; Warthmann, Norman; Henz, Stefan R.; Huson, Daniel H.; Weigel, Detlef

2011-01-01

We present whole-genome assemblies of four divergent Arabidopsis thaliana strains that complement the 125-Mb reference genome sequence released a decade ago. Using a newly developed reference-guided approach, we assembled large contigs from 9 to 42 Gb of Illumina short-read data from the Landsberg erecta (Ler-1), C24, Bur-0, and Kro-0 strains, which have been sequenced as part of the 1,001 Genomes Project for this species. Using alignments against the reference sequence, we first reduced the complexity of the de novo assembly and later integrated reads without similarity to the reference sequence. As an example, half of the noncentromeric C24 genome was covered by scaffolds that are longer than 260 kb, with a maximum of 2.2 Mb. Moreover, over 96% of the reference genome was covered by the reference-guided assembly, compared with only 87% with a complete de novo assembly. Comparisons with 2 Mb of dideoxy sequence reveal that the per-base error rate of the reference-guided assemblies was below 1 in 10,000. Our assemblies provide a detailed, genomewide picture of large-scale differences between A. thaliana individuals, most of which are difficult to access with alignment-consensus methods only. We demonstrate their practical relevance in studying the expression differences of polymorphic genes and show how the analysis of sRNA sequencing data can lead to erroneous conclusions if aligned against the reference genome alone. Genome assemblies, raw reads, and further information are accessible through http://1001genomes.org/projects/assemblies.html. PMID:21646520

Industrial Technology Modernization Program. Project 32. Factory Vision. Phase 2

DTIC Science & Technology

1988-04-01

instructions for the PWA’s, generating the numerical control (NC) program instructions for factory assembly equipment, controlling the process... generating the numerical control (NC) program instructions for factory assembly equipment, controlling the production process instructions and NC... Assembly Operations the "Create Production Process Program" will automatically generate a sequence of graphics pages (in paper mode), or graphics screens

NASA TechPort Entry for Coiled Brine Recovery Assembly (CoBRA) CL IR&D Project

NASA Technical Reports Server (NTRS)

Pensinger, Stuart

2014-01-01

The Coiled Brine Recovery Assembly (CoBRA) project will result in a proof-of-concept demonstration for a lightweight, compact, affordable, regenerable and disposable solution to brine water recovery. The heart of CoBRA is an evaporator that produces water vapor from brine. This evaporator leverages a novel design that enables passive transport of brine from place to place within the system. While it will be necessary to build or modify a system for testing the CoBRA concept, the emphasis of this project will be on developing the evaporator itself. This project will utilize a “test early, test often” approach, building at least one trial evaporator to guide the design of the final product.

Self-assembled nanolaminate coatings (SV)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fan, H.

2012-03-01

Sandia National Laboratories (Sandia) and Lockheed Martin Aeronautics (LM Aero) are collaborating to develop affordable, self-assembled, nanocomposite coatings and associated fabrication processes that will be tailored to Lockheed Martin product requirements. The purpose of this project is to develop a family of self-assembled coatings with properties tailored to specific performance requirements, such as antireflective (AR) optics, using Sandia-developed self-assembled techniques. The project met its objectives by development of a simple and economic self-assembly processes to fabricate multifunctional coatings. Specifically, materials, functionalization methods, and associated coating processes for single layer and multiple layers coatings have been developed to accomplish high reflectivemore » coatings, hydrophobic coatings, and anti-reflective coatings. Associated modeling and simulations have been developed to guide the coating designs for optimum optical performance. The accomplishments result in significant advantages of reduced costs, increased manufacturing freedom/producibility, improved logistics, and the incorporation of new technology solutions not possible with conventional technologies. These self-assembled coatings with tailored properties will significantly address LMC's needs and give LMC a significant competitive lead in new engineered materials. This work complements SNL's LDRD and BES programs aimed at developing multifunctional nanomaterials for microelectronics and optics as well as structure/property investigations of self-assembled nanomaterials. In addition, this project will provide SNL with new opportunities to develop and apply self-assembled nanocomposite optical coatings for use in the wavelength ranges of 3-5 and 8-12 micrometers, ranges of vital importance to military-based sensors and weapons. The SANC technologies will be applied to multiple programs within the LM Company including the F-35, F-22, ADP (Future Strike Bomber, UAV, UCAV, etc.). The SANC technologies will establish LMA and related US manufacturing capability for commercial and military applications therefore reducing reliance on off-shore development and production of related critical technologies. If these technologies are successfully licensed, production of these coatings in manufactory will create significant technical employment opportunities.« less

DOE Office of Scientific and Technical Information (OSTI.GOV)

Michael Viola, J. Edwards, T. Brown, L. Dudek, R. Ellis, P. Heitzenroeder, R. Strykowsky and Michael Cole

The National Compact Stellarator Experiment (NCSX) was a collaborative effort between ORNL and PPPL. PPPL provided the assembly techniques with guidance from ORNL to meet design criteria. The individual vacuum vessel segments, modular coils, trim coils, and toroidal field coils components were delivered to the Field Period Assembly (FPA) crew who then would complete the component assemblies and then assemble the final three field period assemblies, each consisting of two sets of three modular coils assembled over a 120o vacuum vessel segment with the trim coils and toroidal field coils providing the outer layer. The requirements for positioning the modularmore » coils were found to be most demanding. The assembly tolerances required for accurate positioning of the field coil windings in order to generate sufficiently accurate magnetic fields strained state of the art techniques in metrology and alignment and required constant monitoring of assembly steps with laser trackers, measurement arms, and photogrammetry. The FPA activities were being performed concurrently while engineering challenges were being resolved. For example, it was determined that high friction electrically isolated shims were needed between the modular coil interface joints and low distortion welding was required in the nose region of those joints. This took months of analysis and development yet the assembly was not significantly impacted because other assembly tasks could be performed in parallel with ongoing assembly tasks as well as tasks such as advance tooling setup preparation for the eventual welding tasks. The crew technicians developed unique, accurate time saving techniques and tooling which provided significant cost and schedule savings. Project management displayed extraordinary foresight and every opportunity to gain advanced knowledge and develop techniques was taken advantage of. Despite many risk concerns, the cost and schedule performance index was maintained nearly 1.0 during the assembly phase until project cancellation. In this paper, the assembly logic, the engineering challenges, solutions to those challenges and some of the unique and clever assembly techniques, will be presented.« less

[Hybrids of human and monkey adenoviruses (adeno-adeno hybrids) that can reproduce in monkey cells: biological and molecular genetic peculiarities].

PubMed

Grinenko, N F; Savitskaia, N V; Pashvykina, G V; Al'tshteĭn, A D

2003-06-01

A highly oncogenic monkey adenovirus SA7(C8) facilitates the reproduction of human adenovirus type 2 (Ad2) in monkey cells. Upon mixed infection of monkey cells with both viruses, these viruses recombine producing defective adeno-adeno hybrids Ad2C8 serologically identical to Ad2 and capable of assisting Ad2 to reproduce in monkey cells. Ad2C8 and Ad2 form an intercomplementary pair inseparable in monkey cells. Unlike oncogenic SA7(C8), Ad2C8 is a nononcogenic virus for hamsters but is able to induce tumor antigens of this virus (T and TSTA). Molecular genetic analysis of 68 clones of adeno-adeno hybrids revealed that the left part of their genome consists of Ad2 DNA, and the right part contains no less than 40% of the viral SA7(C8) genome where E2A, E3, and E4 genes are located. Apparently, the products of these genes contribute to the composition of adenoviral tumor antigens, while the E4 gene is involved in complementation of monkey and human adenoviruses and makes a contribution to host range determination of these viruses.

Enhanced Incretin Effects of Exendin-4 Expressing Chimeric Plasmid Based On Two-Step Transcription Amplification System with Dendritic Bioreducible Polymer for the Treatment of Type 2 Diabetes

PubMed Central

Kim, Pyung-Hwan; Lee, Minhyung; Nam, Kihoon; Kim, Sung Wan

2014-01-01

Glucagon-like peptide 1 (GLP-1) agonist, exenxdin-4, is currently being advanced as a promising diabetes remedy via a variety of incretin actions similar with GLP-1. In this study, we investigated an effective anti-diabetic therapy via exendin-4 expressing chimeric plasmid based on two-step transcription amplification (TSTA) system with dendrimer-type bioreducible polymer for more improved incretin-based gene therapy. Arginine-grafted poly (cystaminebisacrylamide-diaminohexane) (ABP)-conjugated poly (amido amine) (PAMAM) dendrimer (PAM-ABP) was used as gene carrier. PAM-ABP/chimeric DNA polyplex was markedly elevated exendin-4 expression in ectopic cells as well as increased insulin production through an enhanced activation of protein kinase K (PKA) induced by up-regulation of exendin-4-stimulated cyclic adenosine monophosphate (cAMP) in pancreatic β-cell. Consistent with these results, intravenous administration of PAM-ABP/chimeric DNA polyplex improved glucoregulotory effects, as well as increased insulin secretion by high expression of exendin-4 in blood in type 2 diabetic mice with no any toxicity. Our exendin-4 system can be attributed to provide a potential diabetes therapeutic agent for improved incretin gene therapy. PMID:24839613

[Construction of autocatalytic caspase-3 driven by amplified human telomerase reverse transcriptase promoter and its enhanced efficacy of inducing apoptosis in human ovarian carcinoma].

PubMed

Song, Yue; Shen, Keng; He, Chun-Xia

2007-09-01

To construct recombinant adenoviral vector expressing autocatalysis caspase-3 driven by human telomerase reverse transcriptase promoter amplified by two-step transcription amplification (hTERTp-TSTA), and investigate its antitumor effect in ovarian cancer in vitro and in vivo. Recombinant adenoviruses expressing autocatalytic caspase-3 (rev-caspase-3) driven by hTERTp-TSTA were prepared, which were named as AdHTVP2G5-rev-casp3. AdHT-rev-casp3, Ad-rev-casp3 and AdHTVP2G5-EGEP, which express rev-caspase-3 driven by hTERTp, cytomegalovirus promoter (CMVp) and enhanced green fluorescent protein (EGFP), respectively, were used as controls. Western blot, cell counting kit (CCK-8), flow cytometry (FCM) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) were used to detect the expression of p17, active subunit of caspase-3, and p85, and to measure cell survival rates, apoptotic rates and cell cycle distribution in ovarian cell line AO and normal human umbilical vein endothelial cell line HUVEC, following treatments of AdHTVP2G5-rev-casp3. subcutaneous tumor models and abdominally spread tumor models of human ovarian carcinoma using AO cells in BALB/c nude mice were established. Following treatments of AdHTVP2G5-rev-casp3, western blot was used to detect the expression of active caspase-3 in abdominally spread tumors and liver tissues, respectively, and the mouse survival rates and the volume of tumor nodules were measured, and the serum level of alanine transaminase (ALT) and aspartate transaminase (AST) were analyzed to monitor liver damages and HE staining was used to detect the histopathological changes of various organs. The levels of p17 expression in AdHTVP2G5-rev-casp3-treated AO cells were significantly higher than that in Ad-rev-casp3 or AdHT-rev-casp3 treated AO cells, while no expression was observed in AdHTVP2G5-rev-casp3-treated HUVEC. There was strong cell killing of AdHTVP2G5-rev-casp3 of hTERT positive AO cells, but not of the hTERT-negative HUVEC cells. Cell survival rate and apoptotic rate of AO cells treated with AdHTVP2G5-rev-casp3 were 17.8% and 40.2%, respectively, significantly different from that treated with AdHT-rev-casp3 (75.2% and 16.1%) at the multiplicity of infection (MOI) of 70 (P < 0.01). There was no significant difference in HUVEC cell survival rate and apoptotic rate between AdHTVP2G5-rev-casp3 treatment (97.7% and 2.1%, respectively) and AdHT-rev-casp3 treatment (98.5% and 1.7%, respectively) at the same MOI (P > 0.05). Significant expressions of active caspase-3 were shown in AdHTVP2G5-rev-casp3-treated tumors, whereas no expression was shown in liver. In contrast, both tumors and liver tissues showed active caspase-3 expression following treatments of Ad-rev-casp3. AdHTVP2G5-rev-casp3 and Ad-rev-casp3 prolonged mouse survival [mean survival time of (259 +/- 14) d and (213 +/- 16) d], when compared with treatment with AdHT-rev-casp3 [(177 +/- 12) d] and AdHTVP2G5-EGFP [(109 +/- 7) d; P < 0.01]. The mean volume of AdHTVP2G5-rev-casp3-treated tumor was 406 mm(3), significantly less than those of AdHT-rev-casp3 treatment (990 mm(3)), Ad-rev-casp3 treatment (645 mm(3)) and AdHTVP2G5-EGFP treatment (1728 mm(3); P < 0.01). The serum ALT and AST levels were not significantly elevated in AdHTVP2G5-rev-casp3-treated mice, whereas significantly elevated in Ad-rev-casp3-treated mice. No obvious lesions were found in any organ in AdHTVP2G5-rev-casp-treated group. Recombinant adenovirus AdHTVP2G5-rev-casp3 expressing rev-caspase-3 driven by hTERTp-TSTA can result in marked cell apoptosis with significant tumor targeting, suppressing tumor growth and prolonging the mouse survival, meanwhile, it can prevent against the liver toxicity induced by rev-caspase-3.

Full System Modeling and Validation of the Carbon Dioxide Removal Assembly

NASA Technical Reports Server (NTRS)

Coker, Robert; Knox, James; Gauto, Hernando; Gomez, Carlos

2014-01-01

The Atmosphere Revitalization Recovery and Environmental Monitoring (ARREM) project was initiated in September of 2011 as part of the Advanced Exploration Systems (AES) program. Under the ARREM project, testing of sub-scale and full-scale systems has been combined with multiphysics computer simulations for evaluation and optimization of subsystem approaches. In particular, this paper describes the testing and modeling of various subsystems of the carbon dioxide removal assembly (CDRA). The goal is a full system predictive model of CDRA to guide system optimization and development. The development of the CO2 removal and associated air-drying subsystem hardware under the ARREM project is discussed in a companion paper.

AutoAssemblyD: a graphical user interface system for several genome assemblers.

PubMed

Veras, Adonney Allan de Oliveira; de Sá, Pablo Henrique Caracciolo Gomes; Azevedo, Vasco; Silva, Artur; Ramos, Rommel Thiago Jucá

2013-01-01

Next-generation sequencing technologies have increased the amount of biological data generated. Thus, bioinformatics has become important because new methods and algorithms are necessary to manipulate and process such data. However, certain challenges have emerged, such as genome assembly using short reads and high-throughput platforms. In this context, several algorithms have been developed, such as Velvet, Abyss, Euler-SR, Mira, Edna, Maq, SHRiMP, Newbler, ALLPATHS, Bowtie and BWA. However, most such assemblers do not have a graphical interface, which makes their use difficult for users without computing experience given the complexity of the assembler syntax. Thus, to make the operation of such assemblers accessible to users without a computing background, we developed AutoAssemblyD, which is a graphical tool for genome assembly submission and remote management by multiple assemblers through XML templates. AssemblyD is freely available at https://sourceforge.net/projects/autoassemblyd. It requires Sun jdk 6 or higher.

Self-assembled Nanomaterials for Hybrid Electronic and Photonic Systems

DTIC Science & Technology

2015-05-15

storage media.  Project 3. Self‐assembling Circuit Defect Modeling The self‐assembly of  nanoelectronic  devices provide an opportunity to achieve... nanoelectronics . This work will be useful in predicting the potential success of defect‐ tolerance techniques for DNA self‐assembled  nanoelectronic  substrates...program with integrated circuit emphasis simulations for DNA self-assembled nanoelectronics ." IET Computers and Digital Techniques 3, no. 6 (2009): 553-569.        

Dismantlement of the TSF-SNAP Reactor Assembly

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peretz, Fred J

2009-01-01

This paper describes the dismantlement of the Tower Shielding Facility (TSF)?Systems for Nuclear Auxiliary Power (SNAP) reactor, a SNAP-10A reactor used to validate radiation source terms and shield performance models at Oak Ridge National Laboratory (ORNL) from 1967 through 1973. After shutdown, it was placed in storage at the Y-12 National Security Complex (Y-12), eventually falling under the auspices of the Highly Enriched Uranium (HEU) Disposition Program. To facilitate downblending of the HEU present in the fuel elements, the TSF-SNAP was moved to ORNL on June 24, 2006. The reactor assembly was removed from its packaging, inspected, and the sodium-potassiummore » (NaK) coolant was drained. A superheated steam process was used to chemically react the residual NaK inside the reactor assembly. The heat exchanger assembly was removed from the top of the reactor vessel, and the criticality safety sleeve was exchanged for a new safety sleeve that allowed for the removal of the vessel lid. A chain-mounted tubing cutter was used to separate the lid from the vessel, and the 36 fuel elements were removed and packaged in four U.S. Department of Transportation 2R/6M containers. The fuel elements were returned to Y-12 on July 13, 2006. The return of the fuel elements and disposal of all other reactor materials accomplished the formal objectives of the dismantlement project. In addition, a project model was established for the handling of a fully fueled liquid-metal?cooled reactor assembly. Current criticality safety codes have been benchmarked against experiments performed by Atomics International in the 1950s and 1960s. Execution of this project provides valuable experience applicable to future projects addressing space and liquid-metal-cooled reactors.« less

LCLS-II Cryomodules Production at Fermilab

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arkan, Tug; Grimm, Chuck; Kaluzny, Joshua

2017-05-01

LCLS-II is an upgrade project for the linear coherent light source (LCLS) at SLAC. The LCLS-II linac will consist of thirty-five 1.3 GHz and two 3.9 GHz superconducting RF continuous wave (CW) cryomodules that Fermilab and Jefferson Lab (JLab) will assemble in collaboration with SLAC. The LCLS-II 1.3 GHz cryomodule design is based on the European XFEL pulsed-mode cryomodule design with modifications needed for CW operation. Fermilab and JLab will each assemble and test a prototype 1.3 GHz cryomodule to assess the results of the CW modifications, in advance of 16 and 17 production 1.3 GHz cryomodules, respectively. Fermilab ismore » solely responsible for the 3.9 GHz cryomodules. After the prototype cryomodule tests are complete and lessons learned incorporated, both laboratories will increase their cryomodule production rates to meet the challenging LCLS-II project requirement of approximately one cryomodule per month per laboratory. This paper presents the Fermilab Cryomodule Assembly Facility (CAF) infrastructure for LCLS-II cryomodule production, the Fermilab prototype 1.3 GHz CW cryomodule (pCM) assembly and readiness for production assembly.« less

Mounting apparatus for a nozzle guide vane assembly

DOEpatents

Boyd, G.L.; Shaffer, J.E.

1995-09-12

The present invention provides a ceramic nozzle guide assembly with an apparatus for mounting it to a metal nozzle case that includes an intermediate ceramic mounting ring. The mounting ring includes a plurality of projections that are received within a plurality of receptacles formed in the nozzle case. The projections of the mounting ring are secured within the receptacles by a ceramic retainer that allows contact between the two components only along arcuate surfaces thus eliminating sliding contact between the components. 8 figs.

Industrial Technology Modernization Program. Phase 3 Proposal, Category 1 Project Countermeasures Assembly Improvements

DTIC Science & Technology

1985-05-24

Tracor INDUSTRIAL TECHNOLOGY MODERNIZATION PROGRAM DTICRt .1ECTE CDJUN07 1989 00 PHASE 3 PROPOSAL CATEGORY 1 PROJECT COUNTERMEASURES ASSEMBLY...package in bin C V_ Put-package back in bin C Put part in plastic bag 0CDV _7 _ ] Seal plastic bag with stapler CDDV _ _- 1 Mark paperwork CDV __ I Peel...part in plastic bag CDV7 Seal plastic bag with stapler C>CDV _ Mark paperwork ~CV_ _ Peel preprinted tag from sheet ~ D Put preprinted tag on plastic

Mounting apparatus for a nozzle guide vane assembly

DOEpatents

Boyd, Gary L.; Shaffer, James E.

1995-01-01

The present invention provides a ceramic nozzle guide assembly with an apparatus for mounting it to a metal nozzle case that includes an intermediate ceramic mounting ring. The mounting ring includes a plurality of projections that are received within a plurality of receptacles formed in the nozzle case. The projections of the mounting ring are secured within the receptacles by a ceramic retainer that allows contact between the two components only along arcuate surfaces thus eliminating sliding contact between the components.

Proceedings of the Electronics Manufacturing Seminar (14th Annual) Held in China Lake, California on 21-22 February 1990

DTIC Science & Technology

1990-02-01

Aging effects Aging of metalic surfaces Aqueous cleaning Circuit- card assembly Cleanability Closed-loop soldering Conformal coating Defect...5 Standard Electronic Circuit Card Assembly System ....................................... 7 Douglas Green Lockheed-Sanders Corp. Nashua, New...Facility Naval Weapons Center NAVIRSA Detachment 5 NWC TP 7066 EMPF TR 0010 STANDARD ELECTRONIC CIRCUTT CARD ASSEMBLY SYSTEM (SECAS PROJECT) by Douglas

Scalable Directed Self-Assembly Using Ultrasound Waves

DTIC Science & Technology

2015-09-04

SECURITY CLASSIFICATION OF: We aim to understand how ultrasound waves can be used to create organized patterns of nanoparticles in a host medium such...as a polymer matrix material. The critical difference between the ultrasound technology studied in this project, and other directed self-assembly...of nanoparticles dispersed in a host medium are assembled by means of standing ultrasound waves. Additionally, we have obtained experimental

Center for Ground Vehicle Development and Integration

DTIC Science & Technology

2011-04-22

UNCLASSIFIED OPSEC# 21798 CGVDI Organizational Chart CGVDI Director Project and Operations Management Project Management Operations Management Engineered...Metals Welding Assembly / Paint UNCLASSIFIED UNCLASSIFIED OPSEC# 21798 Project and Operations Management CGVDI serves as a single entry point to RDECOM...for ground vehicle system integration projects, as well as for managing cost, schedule, performance and risk. Project Management Operations

NASA-DoD Lead-Free Electronics Project

NASA Technical Reports Server (NTRS)

Kessel, Kurt

2009-01-01

In response to concerns about risks from lead-free induced faults to high reliability products, NASA has initiated a multi-year project to provide manufacturers and users with data to clarify the risks of lead-free materials in their products. The project will also be of interest to component manufacturers supplying to high reliability markets. The project was launched in November 2006. The primary technical objective of the project is to undertake comprehensive testing to generate information on failure modes/criteria to better understand the reliability of: (1) Packages (e.g., Thin Small Outline Package [TSOP], Ball Grid Array [BGA], Plastic Dual In-line Package [PDIP]) assembled and reworked with solder interconnects consisting of lead-free alloys (2) Packages (e.g., TSOP, BGA, PDIP) assembled and reworked with solder interconnects consisting of mixed alloys, lead component finish/lead-free solder and lead-free component finish/SnPb solder

Assembly: a resource for assembled genomes at NCBI

PubMed Central

Kitts, Paul A.; Church, Deanna M.; Thibaud-Nissen, Françoise; Choi, Jinna; Hem, Vichet; Sapojnikov, Victor; Smith, Robert G.; Tatusova, Tatiana; Xiang, Charlie; Zherikov, Andrey; DiCuccio, Michael; Murphy, Terence D.; Pruitt, Kim D.; Kimchi, Avi

2016-01-01

The NCBI Assembly database (www.ncbi.nlm.nih.gov/assembly/) provides stable accessioning and data tracking for genome assembly data. The model underlying the database can accommodate a range of assembly structures, including sets of unordered contig or scaffold sequences, bacterial genomes consisting of a single complete chromosome, or complex structures such as a human genome with modeled allelic variation. The database provides an assembly accession and version to unambiguously identify the set of sequences that make up a particular version of an assembly, and tracks changes to updated genome assemblies. The Assembly database reports metadata such as assembly names, simple statistical reports of the assembly (number of contigs and scaffolds, contiguity metrics such as contig N50, total sequence length and total gap length) as well as the assembly update history. The Assembly database also tracks the relationship between an assembly submitted to the International Nucleotide Sequence Database Consortium (INSDC) and the assembly represented in the NCBI RefSeq project. Users can find assemblies of interest by querying the Assembly Resource directly or by browsing available assemblies for a particular organism. Links in the Assembly Resource allow users to easily download sequence and annotations for current versions of genome assemblies from the NCBI genomes FTP site. PMID:26578580

Database Initiative Project: Supporting Education Decision Making.

ERIC Educational Resources Information Center

Oregon State Dept. of Education, Salem.

This paper reports on Oregon's Database Initiative Project (DIP). In response to inconsistent financial reports and a lack of automation, a project team was assembled by Oregon's Department of Education in 1997 with the goal of providing detailed school-level data for the 1997-98 school year in 16 Oregonian school districts. Among the project's…

Improved annotation with de novo transcriptome assembly in four social amoeba species.

PubMed

Singh, Reema; Lawal, Hajara M; Schilde, Christina; Glöckner, Gernot; Barton, Geoffrey J; Schaap, Pauline; Cole, Christian

2017-01-31

Annotation of gene models and transcripts is a fundamental step in genome sequencing projects. Often this is performed with automated prediction pipelines, which can miss complex and atypical genes or transcripts. RNA sequencing (RNA-seq) data can aid the annotation with empirical data. Here we present de novo transcriptome assemblies generated from RNA-seq data in four Dictyostelid species: D. discoideum, P. pallidum, D. fasciculatum and D. lacteum. The assemblies were incorporated with existing gene models to determine corrections and improvement on a whole-genome scale. This is the first time this has been performed in these eukaryotic species. An initial de novo transcriptome assembly was generated by Trinity for each species and then refined with Program to Assemble Spliced Alignments (PASA). The completeness and quality were assessed with the Benchmarking Universal Single-Copy Orthologs (BUSCO) and Transrate tools at each stage of the assemblies. The final datasets of 11,315-12,849 transcripts contained 5,610-7,712 updates and corrections to >50% of existing gene models including changes to hundreds or thousands of protein products. Putative novel genes are also identified and alternative splice isoforms were observed for the first time in P. pallidum, D. lacteum and D. fasciculatum. In taking a whole transcriptome approach to genome annotation with empirical data we have been able to enrich the annotations of four existing genome sequencing projects. In doing so we have identified updates to the majority of the gene annotations across all four species under study and found putative novel genes and transcripts which could be worthy for follow-up. The new transcriptome data we present here will be a valuable resource for genome curators in the Dictyostelia and we propose this effective methodology for use in other genome annotation projects.

Genome Annotation Generator: a simple tool for generating and correcting WGS annotation tables for NCBI submission.

PubMed

Geib, Scott M; Hall, Brian; Derego, Theodore; Bremer, Forest T; Cannoles, Kyle; Sim, Sheina B

2018-04-01

One of the most overlooked, yet critical, components of a whole genome sequencing (WGS) project is the submission and curation of the data to a genomic repository, most commonly the National Center for Biotechnology Information (NCBI). While large genome centers or genome groups have developed software tools for post-annotation assembly filtering, annotation, and conversion into the NCBI's annotation table format, these tools typically require back-end setup and connection to an Structured Query Language (SQL) database and/or some knowledge of programming (Perl, Python) to implement. With WGS becoming commonplace, genome sequencing projects are moving away from the genome centers and into the ecology or biology lab, where fewer resources are present to support the process of genome assembly curation. To fill this gap, we developed software to assess, filter, and transfer annotation and convert a draft genome assembly and annotation set into the NCBI annotation table (.tbl) format, facilitating submission to the NCBI Genome Assembly database. This software has no dependencies, is compatible across platforms, and utilizes a simple command to perform a variety of simple and complex post-analysis, pre-NCBI submission WGS project tasks. The Genome Annotation Generator is a consistent and user-friendly bioinformatics tool that can be used to generate a .tbl file that is consistent with the NCBI submission pipeline. The Genome Annotation Generator achieves the goal of providing a publicly available tool that will facilitate the submission of annotated genome assemblies to the NCBI. It is useful for any individual researcher or research group that wishes to submit a genome assembly of their study system to the NCBI.

Genome Annotation Generator: a simple tool for generating and correcting WGS annotation tables for NCBI submission

PubMed Central

Hall, Brian; Derego, Theodore; Bremer, Forest T; Cannoles, Kyle

2018-01-01

Abstract Background One of the most overlooked, yet critical, components of a whole genome sequencing (WGS) project is the submission and curation of the data to a genomic repository, most commonly the National Center for Biotechnology Information (NCBI). While large genome centers or genome groups have developed software tools for post-annotation assembly filtering, annotation, and conversion into the NCBI’s annotation table format, these tools typically require back-end setup and connection to an Structured Query Language (SQL) database and/or some knowledge of programming (Perl, Python) to implement. With WGS becoming commonplace, genome sequencing projects are moving away from the genome centers and into the ecology or biology lab, where fewer resources are present to support the process of genome assembly curation. To fill this gap, we developed software to assess, filter, and transfer annotation and convert a draft genome assembly and annotation set into the NCBI annotation table (.tbl) format, facilitating submission to the NCBI Genome Assembly database. This software has no dependencies, is compatible across platforms, and utilizes a simple command to perform a variety of simple and complex post-analysis, pre-NCBI submission WGS project tasks. Findings The Genome Annotation Generator is a consistent and user-friendly bioinformatics tool that can be used to generate a .tbl file that is consistent with the NCBI submission pipeline Conclusions The Genome Annotation Generator achieves the goal of providing a publicly available tool that will facilitate the submission of annotated genome assemblies to the NCBI. It is useful for any individual researcher or research group that wishes to submit a genome assembly of their study system to the NCBI. PMID:29635297

Definition of large components assembled on-orbit and robot compatible mechanical joints

NASA Technical Reports Server (NTRS)

Williamsen, J.; Thomas, F.; Finckenor, J.; Spiegel, B.

1990-01-01

One of four major areas of project Pathfinder is in-space assembly and construction. The task of in-space assembly and construction is to develop the requirements and the technology needed to build elements in space. A 120-ft diameter tetrahedral aerobrake truss is identified as the focus element. A heavily loaded mechanical joint is designed to robotically assemble the defined aerobrake element. Also, typical large components such as habitation modules, storage tanks, etc., are defined, and attachment concepts of these components to the tetrahedral truss are developed.

Imaging X-Ray Polarimetry Explorer (IXPE) Risk Management

NASA Technical Reports Server (NTRS)

Alexander, Cheryl; Deininger, William D.; Baggett, Randy; Primo, Attina; Bowen, Mike; Cowart, Chris; Del Monte, Ettore; Ingram, Lindsey; Kalinowski, William; Kelley, Anthony;

24 CFR 3280.2 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Development. Bay window—a window assembly whose maximum horizontal projection is not more than two feet from... term does not include any self-propelled recreational vehicle. Calculations used to determine the... 12 U.S.C. 1709(b). Manufactured home construction means all activities relating to the assembly and...

The BOEING 777 - concurrent engineering and digital pre-assembly

DOE Office of Scientific and Technical Information (OSTI.GOV)

Abarbanel, B.

The processes created on the 777 for checking designs were called {open_quotes}digital pre-assembly{close_quotes}. Using FlyThru(tm), a spin-off of a Boeing advanced computing research project, engineers were able to view up to 1500 models (15000 solids) in 3d traversing that data at high speed. FlyThru(tm) was rapidly deployed in 1991 to meet the needs of the 777 for large scale product visualization and verification. The digital pre-assembly process has bad fantastic results. The 777 has had far fewer assembly and systems problems compared to previous airplane programs. Today, FlyThru(tm) is installed on hundreds of workstations on almost every airplane program, andmore » is being used on Space Station, F22, AWACS, and other defense projects. It`s applications have gone far beyond just design review. In many ways, FlyThru is a Data Warehouse supported by advanced tools for analysis. It is today being integrated with Knowledge Based Engineering geometry generation tools.« less

An ethnically relevant consensus Korean reference genome is a step towards personal reference genomes

PubMed Central

Cho, Yun Sung; Kim, Hyunho; Kim, Hak-Min; Jho, Sungwoong; Jun, JeHoon; Lee, Yong Joo; Chae, Kyun Shik; Kim, Chang Geun; Kim, Sangsoo; Eriksson, Anders; Edwards, Jeremy S.; Lee, Semin; Kim, Byung Chul; Manica, Andrea; Oh, Tae-Kwang; Church, George M.; Bhak, Jong

2016-01-01

Human genomes are routinely compared against a universal reference. However, this strategy could miss population-specific and personal genomic variations, which may be detected more efficiently using an ethnically relevant or personal reference. Here we report a hybrid assembly of a Korean reference genome (KOREF) for constructing personal and ethnic references by combining sequencing and mapping methods. We also build its consensus variome reference, providing information on millions of variants from 40 additional ethnically homogeneous genomes from the Korean Personal Genome Project. We find that the ethnically relevant consensus reference can be beneficial for efficient variant detection. Systematic comparison of human assemblies shows the importance of assembly quality, suggesting the necessity of new technologies to comprehensively map ethnic and personal genomic structure variations. In the era of large-scale population genome projects, the leveraging of ethnicity-specific genome assemblies as well as the human reference genome will accelerate mapping all human genome diversity. PMID:27882922

Marshall Space Flight Center's role in EASE/ACCESS mission management

NASA Technical Reports Server (NTRS)

Hawkins, Gerald W.

1987-01-01

The Marshall Space Flight Center (MSFC) Spacelab Payload Project Office was responsible for the mission management and development of several successful payloads. Two recent space construction experiments, the Experimental Assembly of Structures in Extravehicular Activity (EASE) and the Assembly Concept for Construction of Erectable Space Structures (ACCESS), were combined into a payload managed by the center. The Ease/ACCESS was flown aboard the Space Shuttle Mission 61-B. The EASE/ACCESS experiments were the first structures assembled in space, and the method used to manage this successful effort will be useful for future space construction missions. The MSFC mission management responsibilities for the EASE/ACCESS mission are addressed and how the lessons learned from the mission can be applied to future space construction projects are discussed.

Two low coverage bird genomes and a comparison of reference-guided versus de novo genome assemblies.

PubMed

Card, Daren C; Schield, Drew R; Reyes-Velasco, Jacobo; Fujita, Matthew K; Andrew, Audra L; Oyler-McCance, Sara J; Fike, Jennifer A; Tomback, Diana F; Ruggiero, Robert P; Castoe, Todd A

2014-01-01

As a greater number and diversity of high-quality vertebrate reference genomes become available, it is increasingly feasible to use these references to guide new draft assemblies for related species. Reference-guided assembly approaches may substantially increase the contiguity and completeness of a new genome using only low levels of genome coverage that might otherwise be insufficient for de novo genome assembly. We used low-coverage (∼3.5-5.5x) Illumina paired-end sequencing to assemble draft genomes of two bird species (the Gunnison Sage-Grouse, Centrocercus minimus, and the Clark's Nutcracker, Nucifraga columbiana). We used these data to estimate de novo genome assemblies and reference-guided assemblies, and compared the information content and completeness of these assemblies by comparing CEGMA gene set representation, repeat element content, simple sequence repeat content, and GC isochore structure among assemblies. Our results demonstrate that even lower-coverage genome sequencing projects are capable of producing informative and useful genomic resources, particularly through the use of reference-guided assemblies.

Two low coverage bird genomes and a comparison of reference-guided versus de novo genome assemblies

USGS Publications Warehouse

Card, Daren C.; Schield, Drew R.; Reyes-Velasco, Jacobo; Fujita, Matthre K.; Andrew, Audra L.; Oyler-McCance, Sara J.; Fike, Jennifer A.; Tomback, Diana F.; Ruggiero, Robert P.; Castoe, Todd A.

2014-01-01

As a greater number and diversity of high-quality vertebrate reference genomes become available, it is increasingly feasible to use these references to guide new draft assemblies for related species. Reference-guided assembly approaches may substantially increase the contiguity and completeness of a new genome using only low levels of genome coverage that might otherwise be insufficient for de novo genome assembly. We used low-coverage (~3.5–5.5x) Illumina paired-end sequencing to assemble draft genomes of two bird species (the Gunnison Sage-Grouse, Centrocercus minimus, and the Clark's Nutcracker, Nucifraga columbiana). We used these data to estimate de novo genome assemblies and reference-guided assemblies, and compared the information content and completeness of these assemblies by comparing CEGMA gene set representation, repeat element content, simple sequence repeat content, and GC isochore structure among assemblies. Our results demonstrate that even lower-coverage genome sequencing projects are capable of producing informative and useful genomic resources, particularly through the use of reference-guided assemblies.

Hi-rel lead-free printed wiring assemblies

NASA Technical Reports Server (NTRS)

Mehta, A. C.; Bonner, J. K.; Castillo, L. del

2002-01-01

The use of lead in electronics has come under increasing scrutiny. Given the trends in both Japan and Europe, it is highly likely that the U.S. will be driven by commercial interests to phase out of lead in electronics usage. This paper presents data collected on a recent NASA project to focus on finding suitable alternatives to eutectic tin-lead solders and solder pastes. The first phase of this project dealt with determining the most feasible candidates to replace tin-lead and to determine suitable processing operations in assemblies printed wiring boards.

PWR and BWR spent fuel assembly gamma spectra measurements

NASA Astrophysics Data System (ADS)

Vaccaro, S.; Tobin, S. J.; Favalli, A.; Grogan, B.; Jansson, P.; Liljenfeldt, H.; Mozin, V.; Hu, J.; Schwalbach, P.; Sjöland, A.; Trellue, H.; Vo, D.

2016-10-01

A project to research the application of nondestructive assay (NDA) to spent fuel assemblies is underway. The research team comprises the European Atomic Energy Community (EURATOM), embodied by the European Commission, DG Energy, Directorate EURATOM Safeguards; the Swedish Nuclear Fuel and Waste Management Company (SKB); two universities; and several United States national laboratories. The Next Generation of Safeguards Initiative-Spent Fuel project team is working to achieve the following technical goals more easily and efficiently than in the past using nondestructive assay measurements of spent fuel assemblies: (1) verify the initial enrichment, burnup, and cooling time of facility declaration; (2) detect the diversion or replacement of pins, (3) estimate the plutonium mass, (4) estimate the decay heat, and (5) determine the reactivity of spent fuel assemblies. This study focuses on spectrally resolved gamma-ray measurements performed on a diverse set of 50 assemblies [25 pressurized water reactor (PWR) assemblies and 25 boiling water reactor (BWR) assemblies]; these same 50 assemblies will be measured with neutron-based NDA instruments and a full-length calorimeter. Given that encapsulation/repository and dry storage safeguards are the primarily intended applications, the analysis focused on the dominant gamma-ray lines of 137Cs, 154Eu, and 134Cs because these isotopes will be the primary gamma-ray emitters during the time frames of interest to these applications. This study addresses the impact on the measured passive gamma-ray signals due to the following factors: burnup, initial enrichment, cooling time, assembly type (eight different PWR and six different BWR fuel designs), presence of gadolinium rods, and anomalies in operating history. To compare the measured results with theory, a limited number of ORIGEN-ARP simulations were performed.

PWR and BWR spent fuel assembly gamma spectra measurements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vaccaro, S.; Tobin, Stephen J.; Favalli, Andrea

A project to research the application of nondestructive assay (NDA) to spent fuel assemblies is underway. The research team comprises the European Atomic Energy Community (EURATOM), embodied by the European Commission, DG Energy, Directorate EURATOM Safeguards; the Swedish Nuclear Fuel and Waste Management Company (SKB); two universities; and several United States national laboratories. The Next Generation of Safeguards Initiative–Spent Fuel project team is working to achieve the following technical goals more easily and efficiently than in the past using nondestructive assay measurements of spent fuel assemblies: (1) verify the initial enrichment, burnup, and cooling time of facility declaration; (2) detectmore » the diversion or replacement of pins, (3) estimate the plutonium mass, (4) estimate the decay heat, and (5) determine the reactivity of spent fuel assemblies. This study focuses on spectrally resolved gamma-ray measurements performed on a diverse set of 50 assemblies [25 pressurized water reactor (PWR) assemblies and 25 boiling water reactor (BWR) assemblies]; these same 50 assemblies will be measured with neutron-based NDA instruments and a full-length calorimeter. Given that encapsulation/repository and dry storage safeguards are the primarily intended applications, the analysis focused on the dominant gamma-ray lines of 137Cs, 154Eu, and 134Cs because these isotopes will be the primary gamma-ray emitters during the time frames of interest to these applications. This study addresses the impact on the measured passive gamma-ray signals due to the following factors: burnup, initial enrichment, cooling time, assembly type (eight different PWR and six different BWR fuel designs), presence of gadolinium rods, and anomalies in operating history. As a result, to compare the measured results with theory, a limited number of ORIGEN-ARP simulations were performed.« less

PWR and BWR spent fuel assembly gamma spectra measurements

DOE PAGES

Vaccaro, S.; Tobin, Stephen J.; Favalli, Andrea; ...

2016-07-17

A project to research the application of nondestructive assay (NDA) to spent fuel assemblies is underway. The research team comprises the European Atomic Energy Community (EURATOM), embodied by the European Commission, DG Energy, Directorate EURATOM Safeguards; the Swedish Nuclear Fuel and Waste Management Company (SKB); two universities; and several United States national laboratories. The Next Generation of Safeguards Initiative–Spent Fuel project team is working to achieve the following technical goals more easily and efficiently than in the past using nondestructive assay measurements of spent fuel assemblies: (1) verify the initial enrichment, burnup, and cooling time of facility declaration; (2) detectmore » the diversion or replacement of pins, (3) estimate the plutonium mass, (4) estimate the decay heat, and (5) determine the reactivity of spent fuel assemblies. This study focuses on spectrally resolved gamma-ray measurements performed on a diverse set of 50 assemblies [25 pressurized water reactor (PWR) assemblies and 25 boiling water reactor (BWR) assemblies]; these same 50 assemblies will be measured with neutron-based NDA instruments and a full-length calorimeter. Given that encapsulation/repository and dry storage safeguards are the primarily intended applications, the analysis focused on the dominant gamma-ray lines of 137Cs, 154Eu, and 134Cs because these isotopes will be the primary gamma-ray emitters during the time frames of interest to these applications. This study addresses the impact on the measured passive gamma-ray signals due to the following factors: burnup, initial enrichment, cooling time, assembly type (eight different PWR and six different BWR fuel designs), presence of gadolinium rods, and anomalies in operating history. As a result, to compare the measured results with theory, a limited number of ORIGEN-ARP simulations were performed.« less

Project REAL (Real Educational Activities of Learning)

ERIC Educational Resources Information Center

Lindsay, Dolores

1977-01-01

Project REAL (Real Educational Activities of Learning) began as a program for 22 handicapped high school dropouts under age 21 and offered practical instruction in such areas as construction, electronic assembly, and merchandising. (JYC)

Apollo Soyuz Test Project Commemorative plaque in orbit

NASA Image and Video Library

1975-07-17

AST-05-263 (17-18 July 1975) --- The Apollo-Soyuz Test Project (ASTP) Commemorative Plaque is assembled in the Soviet Soyuz Orbital Module during the joint U.S.-USSR Apollo-Soyuz Test Project docking mission in Earth orbit. The plaque is written both in English and Russian.

Experimental Study of an Assembly with Extreme Particulate, Molecular, and Biological Requirements in Different Environmental Scenarios from Quality Point of View

NASA Astrophysics Data System (ADS)

Müller, A.; Urich, D.; Kreck, G.; Metzmacher, M.; Lindner, R.

2018-04-01

The presentation will cover results from an ESA supported investigation to collect lessons learned for mechanism assembly with the focus on quality and contamination requirements verification in exploration projects such as ExoMars.

Comparative genomic data of the Avian Phylogenomics Project.

PubMed

Zhang, Guojie; Li, Bo; Li, Cai; Gilbert, M Thomas P; Jarvis, Erich D; Wang, Jun

2014-01-01

The evolutionary relationships of modern birds are among the most challenging to understand in systematic biology and have been debated for centuries. To address this challenge, we assembled or collected the genomes of 48 avian species spanning most orders of birds, including all Neognathae and two of the five Palaeognathae orders, and used the genomes to construct a genome-scale avian phylogenetic tree and perform comparative genomics analyses (Jarvis et al. in press; Zhang et al. in press). Here we release assemblies and datasets associated with the comparative genome analyses, which include 38 newly sequenced avian genomes plus previously released or simultaneously released genomes of Chicken, Zebra finch, Turkey, Pigeon, Peregrine falcon, Duck, Budgerigar, Adelie penguin, Emperor penguin and the Medium Ground Finch. We hope that this resource will serve future efforts in phylogenomics and comparative genomics. The 38 bird genomes were sequenced using the Illumina HiSeq 2000 platform and assembled using a whole genome shotgun strategy. The 48 genomes were categorized into two groups according to the N50 scaffold size of the assemblies: a high depth group comprising 23 species sequenced at high coverage (>50X) with multiple insert size libraries resulting in N50 scaffold sizes greater than 1 Mb (except the White-throated Tinamou and Bald Eagle); and a low depth group comprising 25 species sequenced at a low coverage (~30X) with two insert size libraries resulting in an average N50 scaffold size of about 50 kb. Repetitive elements comprised 4%-22% of the bird genomes. The assembled scaffolds allowed the homology-based annotation of 13,000 ~ 17000 protein coding genes in each avian genome relative to chicken, zebra finch and human, as well as comparative and sequence conservation analyses. Here we release full genome assemblies of 38 newly sequenced avian species, link genome assembly downloads for the 7 of the remaining 10 species, and provide a guideline of genomic data that has been generated and used in our Avian Phylogenomics Project. To the best of our knowledge, the Avian Phylogenomics Project is the biggest vertebrate comparative genomics project to date. The genomic data presented here is expected to accelerate further analyses in many fields, including phylogenetics, comparative genomics, evolution, neurobiology, development biology, and other related areas.

Positional bias in variant calls against draft reference assemblies.

PubMed

Briskine, Roman V; Shimizu, Kentaro K

2017-03-28

Whole genome resequencing projects may implement variant calling using draft reference genomes assembled de novo from short-read libraries. Despite lower quality of such assemblies, they allowed researchers to extend a wide range of population genetic and genome-wide association analyses to non-model species. As the variant calling pipelines are complex and involve many software packages, it is important to understand inherent biases and limitations at each step of the analysis. In this article, we report a positional bias present in variant calling performed against draft reference assemblies constructed from de Bruijn or string overlap graphs. We assessed how frequently variants appeared at each position counted from ends of a contig or scaffold sequence, and discovered unexpectedly high number of variants at the positions related to the length of either k-mers or reads used for the assembly. We detected the bias in both publicly available draft assemblies from Assemblathon 2 competition as well as in the assemblies we generated from our simulated short-read data. Simulations confirmed that the bias causing variants are predominantly false positives induced by reads from spatially distant repeated sequences. The bias is particularly strong in contig assemblies. Scaffolding does not eliminate the bias but tends to mitigate it because of the changes in variants' relative positions and alterations in read alignments. The bias can be effectively reduced by filtering out the variants that reside in repetitive elements. Draft genome sequences generated by several popular assemblers appear to be susceptible to the positional bias potentially affecting many resequencing projects in non-model species. The bias is inherent to the assembly algorithms and arises from their particular handling of repeated sequences. It is recommended to reduce the bias by filtering especially if higher-quality genome assembly cannot be achieved. Our findings can help other researchers to improve the quality of their variant data sets and reduce artefactual findings in downstream analyses.

SWAP-Assembler 2: Optimization of De Novo Genome Assembler at Large Scale

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meng, Jintao; Seo, Sangmin; Balaji, Pavan

2016-08-16

In this paper, we analyze and optimize the most time-consuming steps of the SWAP-Assembler, a parallel genome assembler, so that it can scale to a large number of cores for huge genomes with the size of sequencing data ranging from terabyes to petabytes. According to the performance analysis results, the most time-consuming steps are input parallelization, k-mer graph construction, and graph simplification (edge merging). For the input parallelization, the input data is divided into virtual fragments with nearly equal size, and the start position and end position of each fragment are automatically separated at the beginning of the reads. Inmore » k-mer graph construction, in order to improve the communication efficiency, the message size is kept constant between any two processes by proportionally increasing the number of nucleotides to the number of processes in the input parallelization step for each round. The memory usage is also decreased because only a small part of the input data is processed in each round. With graph simplification, the communication protocol reduces the number of communication loops from four to two loops and decreases the idle communication time. The optimized assembler is denoted as SWAP-Assembler 2 (SWAP2). In our experiments using a 1000 Genomes project dataset of 4 terabytes (the largest dataset ever used for assembling) on the supercomputer Mira, the results show that SWAP2 scales to 131,072 cores with an efficiency of 40%. We also compared our work with both the HipMER assembler and the SWAP-Assembler. On the Yanhuang dataset of 300 gigabytes, SWAP2 shows a 3X speedup and 4X better scalability compared with the HipMer assembler and is 45 times faster than the SWAP-Assembler. The SWAP2 software is available at https://sourceforge.net/projects/swapassembler.« less

The need for an assembly pilot project

USDA-ARS?s Scientific Manuscript database

Progress has been rapid since the June 2008 start of the cacao genome sequencing project with the completion of the physical map and the accumulation of approximately 10x coverage of the genome with Titanium 454 sequence data of Matina1-6, the highly homozygous Amelonado tree chosen for the project....

Rural transit ITS best practices

DOT National Transportation Integrated Search

2003-03-01

The overall objective of this Best Practices in Rural Transit ITS project was to identify operational best practices and related technology for applying ITS to rural transit. The project team assembled information gathered through case studies to pro...

Swamp Works- Multiple Projects

NASA Technical Reports Server (NTRS)

Carelli, Jonathan M.; Schuler, Jason M.; Chandler, Meredith L.

2013-01-01

My Surface Systems internship over the summer 2013 session covered a broad range of projects that utilized multiple fields of engineering and technology. This internship included a project to create a command center for a 120 ton regolith bin, for the design and assembly of a blast shield to add further protection for the Surface Systems engineers, for the design and assembly of a portable four monitor hyper wall strip that could extend as large as needed, research and programming a nano drill that could be utilized on a next generation robot or rover, and social media tasks including the making of videos, posting to social networking websites and creation of a new outreach program to help spread the word about the Swamp Works laboratory.

Comprehensive evaluation of non-hybrid genome assembly tools for third-generation PacBio long-read sequence data.

PubMed

Jayakumar, Vasanthan; Sakakibara, Yasubumi

2017-11-03

Long reads obtained from third-generation sequencing platforms can help overcome the long-standing challenge of the de novo assembly of sequences for the genomic analysis of non-model eukaryotic organisms. Numerous long-read-aided de novo assemblies have been published recently, which exhibited superior quality of the assembled genomes in comparison with those achieved using earlier second-generation sequencing technologies. Evaluating assemblies is important in guiding the appropriate choice for specific research needs. In this study, we evaluated 10 long-read assemblers using a variety of metrics on Pacific Biosciences (PacBio) data sets from different taxonomic categories with considerable differences in genome size. The results allowed us to narrow down the list to a few assemblers that can be effectively applied to eukaryotic assembly projects. Moreover, we highlight how best to use limited genomic resources for effectively evaluating the genome assemblies of non-model organisms. © The Author 2017. Published by Oxford University Press.

Lightweight IMM PV Flexible Blanket Assembly

NASA Technical Reports Server (NTRS)

Spence, Brian

2015-01-01

Deployable Space Systems (DSS) has developed an inverted metamorphic multijunction (IMM) photovoltaic (PV) integrated modular blanket assembly (IMBA) that can be rolled or z-folded. This IMM PV IMBA technology enables a revolutionary flexible PV blanket assembly that provides high specific power, exceptional stowed packaging efficiency, and high-voltage operation capability. DSS's technology also accommodates standard third-generation triple junction (ZTJ) PV device technologies to provide significantly improved performance over the current state of the art. This SBIR project demonstrated prototype, flight-like IMM PV IMBA panel assemblies specifically developed, designed, and optimized for NASA's high-voltage solar array missions.

Traffic model for commercial payloads in the Materials Experiment Assembly (MEA). [market research in commercial space processing

NASA Technical Reports Server (NTRS)

Tietzel, F. A.

1979-01-01

One hundred individuals representing universities, technical institutes, government agencies, and industrial facilities were surveyed to determine potential commercial use of a self-contained, automated assembly for the space processing of materials during frequent shuttle flights for the 1981 to 1987 period. The approach used and the results of the study are summarized. A time time-phased projection (traffic model) of commercial usage of the materials experiment assembly is provided.

A component modes projection and assembly model reduction methodology for articulated, multi-flexible body structures

NASA Technical Reports Server (NTRS)

Lee, Allan Y.; Tsuha, Walter S.

1993-01-01

A two-stage model reduction methodology, combining the classical Component Mode Synthesis (CMS) method and the newly developed Enhanced Projection and Assembly (EP&A) method, is proposed in this research. The first stage of this methodology, called the COmponent Modes Projection and Assembly model REduction (COMPARE) method, involves the generation of CMS mode sets, such as the MacNeal-Rubin mode sets. These mode sets are then used to reduce the order of each component model in the Rayleigh-Ritz sense. The resultant component models are then combined to generate reduced-order system models at various system configurations. A composite mode set which retains important system modes at all system configurations is then selected from these reduced-order system models. In the second stage, the EP&A model reduction method is employed to reduce further the order of the system model generated in the first stage. The effectiveness of the COMPARE methodology has been successfully demonstrated on a high-order, finite-element model of the cruise-configured Galileo spacecraft.

Opportunity Surroundings on 3,000th Sol, Vertical Projection

NASA Image and Video Library

2012-09-07

This 360-degree vertical projection was assembled from images taken by the navigation camera on NASA Mars Exporation Rover Opportunity shows terrain surrounding the position where the rover spent its 3,000th Martian day.

Opportunity Surroundings on 3,000th Sol, Polar Projection

NASA Image and Video Library

2012-09-07

This 360-degree polar projection was assembled from images taken by the navigation camera on NASA Mars Exporation Rover Opportunity shows terrain surrounding the position where the rover spent its 3,000th Martian day.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reiersen, W.; Heitzenroeder, P.; Neilson, G. H.

The National Compact Stellarator Experiment (NCSX) is being constructed at the Princeton Plasma Physics Laboratory (PPPL) in partnership with the Oak Ridge National Laboratory (ORNL). The stellarator core is designed to produce a compact 3-D plasma that combines stellarator and tokamak physics advantages. The engineering challenges of NCSX stem from its complex geometry. From the project's start in April, 2003 to September, 2004, the fabrication specifications for the project's two long-lead components, the modular coil winding forms and the vacuum vessel, were developed. An industrial manufacturing R&D program refined the processes for their fabrication as well as production cost andmore » schedule estimates. The project passed a series of reviews and established its performance baseline with the Department of Energy. In September 2004, fabrication was approved and contracts for these components were awarded. The suppliers have completed the engineering and tooling preparations and are in production. Meanwhile, the project completed preparations for winding the coils at PPPL by installing a coil manufacturing facility and developing all necessary processes through R&D. The main activities for the next two years will be component manufacture, coil winding, and sub-assembly of the vacuum vessel and coil subsets. Machine sector sub-assembly, machine assembly, and testing will follow, leading to First Plasma in July 2009.« less

Present status and future prospects of the JT-60SA project

NASA Astrophysics Data System (ADS)

Ishida, S.; Barabaschi, P.; Kamada, Y.

2014-10-01

The JT-60SA project has been implemented jointly by Europe and Japan since June 2007. After the disassembly of JT-60 from the torus hall had been completed in October 2012, the project achieved the major milestone of starting the tokamak's assembly at the JAEA Naka site in January 2013 following the completion of the cryostat base in Europe and its transport to Japan. Procurement and assembly activities for components such as the superconducting magnet, cryogenic system, power supply, vacuum vessel, divertor and cryostat are progressing on track towards the start of operation in March 2019. In preparation for exploitation, the JT-60SA Research Plan was issued in December 2011, and the research integration activities are addressing JT-60SA data management, validation and analysis tools. This paper overviews the latest evolution of the project in terms of construction and exploitation for JT-60SA.

Final Report - High Performance, Durable, Low Cost Membrane Electrode Assemblies for Transportation Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Steinbach, Andrew

2017-05-31

The primary project objective was development of improved polymer electrolyte membrane fuel cell (PEMFC) membrane electrode assemblies (MEAs) which address the key DOE barriers of performance, durability and cost. Additional project objectives were to address commercialization barriers specific to MEAs comprising 3M nanostructured thin film (NSTF) electrodes, including a larger-than-acceptable sensitivity to operating conditions, an unexplained loss of rated power capability with operating time, and slow break-in conditioning. Significant progress was made against each of these barriers, and most DOE 2020 targets were met or substantially approached.

Implementation of an ergonomics program for the welding department inside a car assembly company.

PubMed

Francisco, Caceres; Edwin, Troya

2012-01-01

The premise for this project is to implement an ergonomics program for the welding department of a car assembly company, considering that this area represents the highest occupational risk in relation to musculoskeletal injuries. The project also allows the demonstration of the different implementation processes of an ergonomics program; it also permits the determination of individual risks faced by workers of the welding department, and finally gives a chance of improving common injuries using the sentinel system. The main challenge is to reduce musculoskeletal injuries and absenteeism among welders through actions implemented by the ergonomics program.

Passive gamma analysis of the boiling-water-reactor assemblies

NASA Astrophysics Data System (ADS)

Vo, D.; Favalli, A.; Grogan, B.; Jansson, P.; Liljenfeldt, H.; Mozin, V.; Schwalbach, P.; Sjöland, A.; Tobin, S.; Trellue, H.; Vaccaro, S.

2016-09-01

This research focused on the analysis of a set of stationary passive gamma measurements taken on the spent nuclear fuel assemblies from a boiling water reactor (BWR) using pulse height analysis data acquisition. The measurements were performed on 25 different BWR assemblies in 2014 at Sweden's Central Interim Storage Facility for Spent Nuclear Fuel (Clab). This study was performed as part of the Next Generation of Safeguards Initiative-Spent Fuel project to research the application of nondestructive assay (NDA) to spent fuel assemblies. The NGSI-SF team is working to achieve the following technical goals more easily and efficiently than in the past using nondestructive assay (NDA) measurements of spent fuel assemblies: (1) verify the initial enrichment, burnup, and cooling time of facility declaration; (2) detect the diversion or replacement of pins, (3) estimate the plutonium mass, (4) estimate the decay heat, and (5) determine the reactivity of spent fuel assemblies. The final objective of this project is to quantify the capability of several integrated NDA instruments to meet the aforementioned goals using the combined signatures of neutrons, gamma rays, and heat. This report presents a selection of the measured data and summarizes an analysis of the results. Specifically, trends in the count rates measured for spectral lines from the following isotopes were analyzed as a function of the declared burnup and cooling time: 137Cs, 154Eu, 134Cs, and to a lesser extent, 106Ru and 144Ce. From these measured count rates, predictive algorithms were developed to enable the estimation of the burnup and cooling time. Furthermore, these algorithms were benchmarked on a set of assemblies not included in the standard assemblies set used by this research team.

Passive gamma analysis of the boiling-water-reactor assemblies

DOE PAGES

Vo, D.; Favalli, A.; Grogan, B.; ...

2016-09-01

This research focused on the analysis of a set of stationary passive gamma measurements taken on the spent nuclear fuel assemblies from a boiling water reactor (BWR) using pulse height analysis data acquisition. The measurements were performed on 25 different BWR assemblies in 2014 at Sweden’s Central Interim Storage Facility for Spent Nuclear Fuel (Clab). This study was performed as part of the Next Generation of Safeguards Initiative–Spent Fuel project to research the application of nondestructive assay (NDA) to spent fuel assemblies. The NGSI–SF team is working to achieve the following technical goals more easily and efficiently than in themore » past using nondestructive assay (NDA) measurements of spent fuel assemblies: (1) verify the initial enrichment, burnup, and cooling time of facility declaration; (2) detect the diversion or replacement of pins, (3) estimate the plutonium mass, (4) estimate the decay heat, and (5) determine the reactivity of spent fuel assemblies. The final objective of this project is to quantify the capability of several integrated NDA instruments to meet the aforementioned goals using the combined signatures of neutrons, gamma rays, and heat. This report presents a selection of the measured data and summarizes an analysis of the results. Specifically, trends in the count rates measured for spectral lines from the following isotopes were analyzed as a function of the declared burnup and cooling time: 137Cs, 154Eu, 134Cs, and to a lesser extent, 106Ru and 144Ce. From these measured count rates, predictive algorithms were developed to enable the estimation of the burnup and cooling time. Furthermore, these algorithms were benchmarked on a set of assemblies not included in the standard assemblies set used by this research team.« less

Two Low Coverage Bird Genomes and a Comparison of Reference-Guided versus De Novo Genome Assemblies

PubMed Central

Card, Daren C.; Schield, Drew R.; Reyes-Velasco, Jacobo; Fujita, Matthew K.; Andrew, Audra L.; Oyler-McCance, Sara J.; Fike, Jennifer A.; Tomback, Diana F.; Ruggiero, Robert P.; Castoe, Todd A.

2014-01-01

As a greater number and diversity of high-quality vertebrate reference genomes become available, it is increasingly feasible to use these references to guide new draft assemblies for related species. Reference-guided assembly approaches may substantially increase the contiguity and completeness of a new genome using only low levels of genome coverage that might otherwise be insufficient for de novo genome assembly. We used low-coverage (∼3.5–5.5x) Illumina paired-end sequencing to assemble draft genomes of two bird species (the Gunnison Sage-Grouse, Centrocercus minimus, and the Clark's Nutcracker, Nucifraga columbiana). We used these data to estimate de novo genome assemblies and reference-guided assemblies, and compared the information content and completeness of these assemblies by comparing CEGMA gene set representation, repeat element content, simple sequence repeat content, and GC isochore structure among assemblies. Our results demonstrate that even lower-coverage genome sequencing projects are capable of producing informative and useful genomic resources, particularly through the use of reference-guided assemblies. PMID:25192061

Semi-Automatic In Silico Gap Closure Enabled De Novo Assembly of Two Dehalobacter Genomes from Metagenomic Data

PubMed Central

Tang, Shuiquan; Gong, Yunchen; Edwards, Elizabeth A.

2012-01-01

Typically, the assembly and closure of a complete bacterial genome requires substantial additional effort spent in a wet lab for gap resolution and genome polishing. Assembly is further confounded by subspecies polymorphism when starting from metagenome sequence data. In this paper, we describe an in silico gap-resolution strategy that can substantially improve assembly. This strategy resolves assembly gaps in scaffolds using pre-assembled contigs, followed by verification with read mapping. It is capable of resolving assembly gaps caused by repetitive elements and subspecies polymorphisms. Using this strategy, we realized the de novo assembly of the first two Dehalobacter genomes from the metagenomes of two anaerobic mixed microbial cultures capable of reductive dechlorination of chlorinated ethanes and chloroform. Only four additional PCR reactions were required even though the initial assembly with Newbler v. 2.5 produced 101 contigs within 9 scaffolds belonging to two Dehalobacter strains. By applying this strategy to the re-assembly of a recently published genome of Bacteroides, we demonstrate its potential utility for other sequencing projects, both metagenomic and genomic. PMID:23284863

Physics Laboratory Project Book, 1979-80.

ERIC Educational Resources Information Center

Connecticut State Dept. of Education, Hartford. Bureau of Vocational-Technical Schools.

This Physics Laboratory Project Book, assembled through a survey of science instructors in vocational-technical schools in Connecticut, is an extension of the Chemistry-Materials Laboratory Project Book (see note) and is intended to meet a variety of needs. It can serve as an idea book, with the instructor taking from it as needed and adding or…

K-6 Early Intervention Project: Evaluation of the First Year of Implementation.

ERIC Educational Resources Information Center

McDaid, Janet L.

California's K-6 Early Intervention Project was established in 1986 under the authority of California Assembly Bill 1535, Pilot Project on Hyperactivity, to provide a system of early identification and assistance to children in kindergarten through grade 6 whose behavior problems interfere with learning. This report contains evaluation findings…

Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis

PubMed Central

Dow, Eliot; Siletti, Kimberly

2015-01-01

The assembly of a nervous system requires the extension of axons and dendrites to specific regions where they are matched with appropriate synaptic targets. Although the cues that guide long-range outgrowth have been characterized extensively, additional mechanisms are required to explain short-range guidance in neural development. Using a complementary combination of time-lapse imaging by fluorescence confocal microscopy and serial block-face electron microscopy, we identified a novel type of presynaptic projection that participates in the assembly of the vertebrate nervous system. Synapse formation by each hair cell of the zebrafish's lateral line occurs during a particular interval after the cell's birth. During the same period, projections emerge from the cellular soma, extending toward a specific subpopulation of mature hair cells and interacting with polarity-specific afferent nerve terminals. The terminals then extend along the projections to reach appropriately matched presynaptic sites, after which the projections recede. Our results suggest that presynaptic projections act as transient scaffolds for short-range partner matching, a mechanism that may occur elsewhere in the nervous system. PMID:25995190

Exploiting long read sequencing technologies to establish high quality highly contiguous pig reference genome assemblies

USDA-ARS?s Scientific Manuscript database

The current pig reference genome sequence (Sscrofa10.2) was established using Sanger sequencing and following the clone-by-clone hierarchical shotgun sequencing approach used in the public human genome project. However, as sequence coverage was low (4-6x) the resulting assembly was only of draft qua...

Method and apparatus for performing in-situ vacuum-assisted metal to glass sealing

DOEpatents

Kramer, D.P.; Massey, R.T.

1985-07-18

A method and apparatus for assembling and fusing glass to metal in a glass-metal electrical component is disclosed. The component includes a metallic shell formed with upper and lower cylindrical recesses connected together by longitudinal passages, a pair of metal rings and plural metal pins assembled to define electrical feed-throughs. The component parts are assembled on a fixture having a sleeve-like projection and a central mounting projection establishing concentric nesting surfaces to which the metal rings are slip-fitted in concentric alignment with each other spaced from sidewalls of the lower recess. The pins are in electrical contact with the metal rings. A glass pre-form is seated within the upper recess. The assembled structure is heated to a temperature sufficient to melt the glass pre-form which flows under gravity through the passages into the lower recess to provide an insulative seal between the metal parts. The gravity flow of glass is assisted by applying vacuum to the lower recess, ensuring that all spaces between the metal parts are filled with sealing glass without formation of bubbles.

Method and apparatus for performing in-situ vacuum-assisted metal to glass sealing

DOEpatents

Kramer, Daniel P.; Massey, Richard T.

1986-01-01

A method and apparatus for assembling and fusing glass to metal in a glass-metal electrical component is disclosed. The component includes a metallic shell formed with upper and lower cylindrical recesses connected together by longitudinal passages, a pair of metal rings and plural metal pins assembled to define electrical feed-throughs. The component parts are assembled on a fixture having a sleeve-like projection and a central mounting projection establishing concentric nesting surfaces to which the metal rings are slip-fitted in concentric alignment with each other spaced from sidewalls of the lower recess. The pins are in electrical contact with the metal rings. A glass pre-form is seated within the upper recess. The assembled structure is heated to a temperature sufficient to melt the glass pre-form which flows under gravity through the passages into the lower recess to provide an insulative seal between the metal parts. The gravity flow of glass is assisted by applying vacuum to the lower recess, ensuring that all spaces between the metal parts are filled with sealing glass without formation of bubbles.

KSC-2010-4885

NASA Image and Video Library

2010-09-28

CAPE CANAVERAL, Fla. -- At NASA's Kennedy Space Center in Florida, Bill McArthur, (left) Space Shuttle Program Orbiter Projects manager; John Casper, Assistant Space Shuttle Program manager; John Shannon, Space Shuttle Program manager and Canadian Space Agency astronaut Chris Hadfield attend a ceremony being held to commemorate the move from Kennedy's Assembly Refurbishment Facility (ARF) to the Vehicle Assembly Building (VAB) of the Space Shuttle Program's final solid rocket booster structural assembly -- the right-hand forward. The move was postponed because of inclement weather. Photo credit: NASA/Kim Shiflett

Hybrid Wing Body Multi-Bay Test Article Analysis and Assembly Final Report

NASA Technical Reports Server (NTRS)

Velicki, Alexander; Hoffman, Krishna; Linton, Kim A.; Baraja, Jaime; Wu, Hsi-Yung T.; Thrash, Patrick

2017-01-01

This report summarizes work performed by The Boeing Company, through its Boeing Research & Technology organization located in Huntington Beach, California, under the Environmentally Responsible Aviation (ERA) project. The report documents work performed to structurally analyze and assemble a large-scale Multi-bay Box (MBB) Test Article capable of withstanding bending and internal pressure loadings representative of a Hybrid Wing Body (HWB) aircraft. The work included fabrication of tooling elements for use in the fabrication and assembly of the test article.

Institutional environmental impact statement, Michoud Assembly Facility, New Orleans, Louisiana

NASA Technical Reports Server (NTRS)

1978-01-01

A description and analysis of Michoud Assembly Facility as an operational base for both NASA and NASA-related programs and various government tenant-agencies and their contractors is given. Tenant-agencies are governmental agencies or governmental agency contractors which are not involved in a NASA program, but utilize office or manufacturing space at the Michoud Assembly Facility. The statements represent the full description of the likely environmental effects of the facility and are used in the process of making program and project decisions.

Detachable connection for a nuclear reactor fuel assembly

DOEpatents

Christiansen, D.W.; Karnesky, R.A.

1983-08-29

A locking connection for releasably attaching a handling socket to the duct tube of a fuel assembly for a nuclear reactor. The connection comprises a load pad housing mechanically attached to the duct tube and a handling socket threadably secured within the housing. A retaining ring is interposed between the housing and the handling socket and is formed with a projection and depression engagable within a cavity and groove of the housing and handling socket, respectively, to form a detachable interlocked connection assembly.

Design and Application of Integrated Assembly Technology of FRG in Residential Ceiling

NASA Astrophysics Data System (ADS)

Li, Xiuyun; Yu, Changyong

2018-06-01

FRG material is a new environmentally friendly indoor decoration materials and popular in prefabricated construction, the paper introduces the performance and design of materials, and takes FRG in the residential ceiling integrated assembly process into a demonstration project, which showed that FRG in the prefabricated modules integrated ceiling of the whole template scheme has a great artistry and application effect. Meanwhile it provides reference for the integrated ceiling assembly modular process design of similar indoor decoration.

Detachable connection for a nuclear reactor fuel assembly

DOEpatents

Christiansen, David W.; Karnesky, Richard A.

1986-01-01

A locking connection for releasably attaching a handling socket to the duct tube of a fuel assembly for a nuclear reactor. The connection comprises a load pad housing mechanically attached to the duct tube and a handling socket threadably secured within the housing. A retaining ring is interposed between the housing and the handling socket and is formed with a projection and depression engageable within a cavity and groove of the housing and handling socket, respectively, to form a detachable interlocked connection assembly.

Systematic Review Methodology for the Fatigue in Emergency Medical Services Project

DOT National Transportation Integrated Search

2018-01-11

Background: Guidance for managing fatigue in the Emergency Medical Services (EMS) setting is limited. The Fatigue in EMS Project sought to complete multiple systematic reviews guided by seven explicit research questions, assemble the best available e...

TxDOT ITS data archiving research project activities

DOT National Transportation Integrated Search

2001-09-01

The objective of this project was to assemble guidance for the Texas Department of Trasportation (TxDOT) in further developing data archiving systems across the state. This guidance, which is contained in the companion Report 2127-3, includes informa...

Integrating genome assemblies with MAIA

PubMed Central

Nijkamp, Jurgen; Winterbach, Wynand; van den Broek, Marcel; Daran, Jean-Marc; Reinders, Marcel; de Ridder, Dick

2010-01-01

Motivation: De novo assembly of a eukaryotic genome with next-generation sequencing data is still a challenging task. Over the past few years several assemblers have been developed, often suitable for one specific type of sequencing data. The number of known genomes is expanding rapidly, therefore it becomes possible to use multiple reference genomes for assembly projects. We introduce an assembly integrator that makes use of all available data, i.e. multiple de novo assemblies and mappings against multiple related genomes, by optimizing a weighted combination of criteria. Results: The developed algorithm was applied on the de novo sequencing of the Saccharomyces cerevisiae CEN.PK 113-7D strain. Using Solexa and 454 read data, two de novo and three comparative assemblies were constructed and subsequently integrated, yielding 29 contigs, covering more than 12 Mbp; a drastic improvement compared with the single assemblies. Availability: MAIA is available as a Matlab package and can be downloaded from http://bioinformatics.tudelft.nl Contact: j.f.nijkamp@tudelft.nl PMID:20823304

Hawkeye and AMOS: visualizing and assessing the quality of genome assemblies

PubMed Central

Schatz, Michael C.; Phillippy, Adam M.; Sommer, Daniel D.; Delcher, Arthur L.; Puiu, Daniela; Narzisi, Giuseppe; Salzberg, Steven L.; Pop, Mihai

2013-01-01

Since its launch in 2004, the open-source AMOS project has released several innovative DNA sequence analysis applications including: Hawkeye, a visual analytics tool for inspecting the structure of genome assemblies; the Assembly Forensics and FRCurve pipelines for systematically evaluating the quality of a genome assembly; and AMOScmp, the first comparative genome assembler. These applications have been used to assemble and analyze dozens of genomes ranging in complexity from simple microbial species through mammalian genomes. Recent efforts have been focused on enhancing support for new data characteristics brought on by second- and now third-generation sequencing. This review describes the major components of AMOS in light of these challenges, with an emphasis on methods for assessing assembly quality and the visual analytics capabilities of Hawkeye. These interactive graphical aspects are essential for navigating and understanding the complexities of a genome assembly, from the overall genome structure down to individual bases. Hawkeye and AMOS are available open source at http://amos.sourceforge.net. PMID:22199379

A computational genomics pipeline for prokaryotic sequencing projects.

PubMed

Kislyuk, Andrey O; Katz, Lee S; Agrawal, Sonia; Hagen, Matthew S; Conley, Andrew B; Jayaraman, Pushkala; Nelakuditi, Viswateja; Humphrey, Jay C; Sammons, Scott A; Govil, Dhwani; Mair, Raydel D; Tatti, Kathleen M; Tondella, Maria L; Harcourt, Brian H; Mayer, Leonard W; Jordan, I King

2010-08-01

New sequencing technologies have accelerated research on prokaryotic genomes and have made genome sequencing operations outside major genome sequencing centers routine. However, no off-the-shelf solution exists for the combined assembly, gene prediction, genome annotation and data presentation necessary to interpret sequencing data. The resulting requirement to invest significant resources into custom informatics support for genome sequencing projects remains a major impediment to the accessibility of high-throughput sequence data. We present a self-contained, automated high-throughput open source genome sequencing and computational genomics pipeline suitable for prokaryotic sequencing projects. The pipeline has been used at the Georgia Institute of Technology and the Centers for Disease Control and Prevention for the analysis of Neisseria meningitidis and Bordetella bronchiseptica genomes. The pipeline is capable of enhanced or manually assisted reference-based assembly using multiple assemblers and modes; gene predictor combining; and functional annotation of genes and gene products. Because every component of the pipeline is executed on a local machine with no need to access resources over the Internet, the pipeline is suitable for projects of a sensitive nature. Annotation of virulence-related features makes the pipeline particularly useful for projects working with pathogenic prokaryotes. The pipeline is licensed under the open-source GNU General Public License and available at the Georgia Tech Neisseria Base (http://nbase.biology.gatech.edu/). The pipeline is implemented with a combination of Perl, Bourne Shell and MySQL and is compatible with Linux and other Unix systems.

Orbiter/External Tank Mate 3-D Solid Modeling

NASA Technical Reports Server (NTRS)

Godfrey, G. S.; Brandt, B.; Rorden, D.; Kapr, F.

2004-01-01

This research and development project presents an overview of the work completed while attending a summer 2004 American Society of Engineering Education/National Aeronautics and Space Administration (ASEE/NASA) Faculty Fellowship. This fellowship was completed at the Kennedy Space Center, Florida. The scope of the project was to complete parts, assemblies, and drawings that could be used by Ground Support Equipment (GSE) personnel to simulate situations and scenarios commonplace to the space shuttle Orbiter/External Tank (ET) Mate (50004). This mate takes place in the Vehicle Assembly Building (VAB). These simulations could then be used by NASA engineers as decision-making tools. During the summer of 2004, parts were created that defined the Orbiter/ET structural interfaces. Emphasis was placed upon assemblies that included the Orbiter/ET forward attachment (EO-1), aft left thrust strut (EO-2), aft right tripod support structure (EO-3), and crossbeam and aft feedline/umbilical supports. These assemblies are used to attach the Orbiter to the ET. The Orbiter/ET Mate assembly was then used to compare and analyze clearance distances using different Orbiter hang angles. It was found that a 30-minute arc angle change in Orbiter hang angle affected distance at the bipod strut to Orbiter yoke fitting 8.11 inches. A 3-D solid model library was established as a result of this project. This library contains parts, assemblies, and drawings translated into several formats. This library contains a collection of the following files: sti for sterolithography, stp for neutral file work, shrinkwrap for compression. tiff for photoshop work, jpeg for Internet use, and prt and asm for Pro/Engineer use. This library was made available to NASA engineers so that they could access its contents to make angle, load, and clearance analysis studies. These decision-making tools may be used by Pro/Engineer users and non-users.

Parallel In Vivo DNA Assembly by Recombination: Experimental Demonstration and Theoretical Approaches

PubMed Central

Shi, Zhenyu; Wedd, Anthony G.; Gras, Sally L.

2013-01-01

The development of synthetic biology requires rapid batch construction of large gene networks from combinations of smaller units. Despite the availability of computational predictions for well-characterized enzymes, the optimization of most synthetic biology projects requires combinational constructions and tests. A new building-brick-style parallel DNA assembly framework for simple and flexible batch construction is presented here. It is based on robust recombination steps and allows a variety of DNA assembly techniques to be organized for complex constructions (with or without scars). The assembly of five DNA fragments into a host genome was performed as an experimental demonstration. PMID:23468883

Accelerated Thermal Cycling and Failure Mechanisms for BGA and CSP Assemblies

NASA Technical Reports Server (NTRS)

Ghaffarian, Reza

2000-01-01

This paper reviews the accelerated thermal cycling test methods that are currently used by industry to characterize the interconnect reliability of commercial-off-the-shelf (COTS) ball grid array (BGA) and chip scale package (CSP) assemblies. Acceleration induced failure mechanisms varied from conventional surface mount (SM) failures for CSPs. Examples of unrealistic life projections for other CSPs are also presented. The cumulative cycles to failure for ceramic BGA assemblies performed under different conditions, including plots of their two Weibull parameters, are presented. The results are for cycles in the range of -30 C to 100 C, -55 C to 100 C, and -55 C to 125 C. Failure mechanisms as well as cycles to failure for thermal shock and thermal cycling conditions in the range of -55 C to 125 C were compared. Projection to other temperature cycling ranges using a modified Coffin-Manson relationship is also presented.

Collagen peptide-based biomaterials for protein delivery and peptide-promoted self-assembly of gold nanoparticles

NASA Astrophysics Data System (ADS)

Ernenwein, Dawn M.

2011-12-01

Bottom-up self-assembly of peptides has driven the research progress for the following two projects: protein delivery vehicles of collagen microflorettes and the assembly of gold nanoparticles with coiled-coil peptides. Collagen is the most abundant protein in the mammals yet due to immunogenic responses, batch-to-batch variability and lack of sequence modifications, synthetic collagen has been designed to self-assemble into native collagen-like structures. In particular with this research, metal binding ligands were incorporated on the termini of collagen-like peptides to generate micron-sized particles, microflorettes. The over-arching goal of the first research project is to engineer MRI-active microflorettes, loaded with His-tagged growth factors with differential release rates while bound to stem cells that can be implemented toward regenerative cell-based therapies. His-tagged proteins, such as green fluorescent protein, have successfully been incorporated on the surface and throughout the microflorettes. Protein release was monitored under physiological conditions and was related to particle degradation. In human plasma full release was obtained within six days. Stability of the microflorettes under physiological conditions was also examined for the development of a therapeutically relevant delivery agent. Additionally, MRI active microflorettes have been generated through the incorporation of a gadolinium binding ligand, DOTA within the collagen-based peptide sequence. To probe peptide-promoted self-assemblies of gold nanoparticles (GNPs) by non-covalent, charge complementary interactions, a highly anionic coiled-coil peptide was designed and synthesized. Upon formation of peptide-GNP interactions, the hydrophobic domain of the coiled-coil were shown to promote the self-assembly of peptide-GNPs clustering. Hydrophobic forces were found to play an important role in the assembly process, as a peptide with an equally overall negative charge, but lacking an ordered hydrophobic face had no effect on GNP assembly. The self-assembly system herein is advantageous due to its reversible nature upon addition of high salt concentrations which masks the surface charge. There is great potential for using this uniquely designed self-assembled peptide-gold nanoparticle system for exploring the interplay between peptide ligation and GNP self-assembly.

Characterization of thermal-hydraulic and ignition phenomena in prototypic, full-length boiling water reactor spent fuel pool assemblies after a complete loss-of-coolant accident.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lindgren, Eric Richard; Durbin, Samuel G

2007-04-01

The objective of this project was to provide basic thermal-hydraulic data associated with a SFP complete loss-of-coolant accident. The accident conditions of interest for the SFP were simulated in a full-scale prototypic fashion (electrically-heated, prototypic assemblies in a prototypic SFP rack) so that the experimental results closely represent actual fuel assembly responses. A major impetus for this work was to facilitate code validation (primarily MELCOR) and reduce questions associated with interpretation of the experimental results. It was necessary to simulate a cluster of assemblies to represent a higher decay (younger) assembly surrounded by older, lower-power assemblies. Specifically, this program providedmore » data and analysis confirming: (1) MELCOR modeling of inter-assembly radiant heat transfer, (2) flow resistance modeling and the natural convective flow induced in a fuel assembly as it heats up in air, (3) the potential for and nature of thermal transient (i.e., Zircaloy fire) propagation, and (4) mitigation strategies concerning fuel assembly management.« less

Reuse of assembly systems: a great ecological and economical potential for facility suppliers

NASA Astrophysics Data System (ADS)

Weule, Hartmut; Buchholz, Carsten

2001-02-01

In addition to the consumer goods, capital goods offer a great potential for ecological and economic optimization. In view of this fact the project WiMonDi (Re-Use of Assembly Systems as new Business Fields), started in September 1998, focuses a marketable Remanufacturing and Re-Use of modules and components of assembly systems by using technically and organizationally continuous concepts. The objective of the closed Facility-Management-System is to prolong the serviceable lifespan of assembly facilities through the organized dismantling, refurbishment and reconditioning of the assembly facilities as well as their components. Therefore, it is necessary to develop easible and methodical strategies to realize a workable Re-Use concept. Within the project the focus is based on the optimization of Re-Use-strategies - the direct Re-Use, the Re-Use including Refurbishment as well as Material Recycling. The decision for an optimal strategy depends on economical (e.g. residual value, cost/benefit of relevant processes, etc.), ecological (e.g. pollutant components /substances), etc.) and technical parameters (e.g. reliability, etc.). For the purpose to integrate the total cost-of-ownership of products or components, WiMonDi integrates the costs of the use of products as well as the Re-Use costs/benefits. To initiate the conception of new distribution and user models between the supplier and the user of assembly facilities the described approach is conducted in close cooperation between Industry and University.

Neutron Collar Evolution and Fresh PWR Assembly Measurements with a New Fast Neutron Passive Collar

DOE Office of Scientific and Technical Information (OSTI.GOV)

Menlove, Howard Olsen; Geist, William H.; Root, Margaret A.

The passive neutron collar approach removes the effect of poison rods when using a 1mm Gd liner. This project sets out to solve the following challenges: BWR fuel assemblies have less mass and less neutron multiplication than PWR; and effective removal of cosmic ray spallation neutron bursts needed via QC tests.

Evaluating Virtual Reality and Augmented Reality Training for Industrial Maintenance and Assembly Tasks

ERIC Educational Resources Information Center

Gavish, Nirit; Gutiérrez, Teresa; Webel, Sabine; Rodríguez, Jorge; Peveri, Matteo; Bockholt, Uli; Tecchia, Franco

2015-01-01

The current study evaluated the use of virtual reality (VR) and augmented reality (AR) platforms, developed within the scope of the SKILLS Integrated Project, for industrial maintenance and assembly (IMA) tasks training. VR and AR systems are now widely regarded as promising training platforms for complex and highly demanding IMA tasks. However,…

ARO-YIP (Materials By Design): Organic Photovoltaic Multiferroics

DTIC Science & Technology

Materials-by- design and self-assembly principles are applied to organic functional materials to control their morphology, interface, and crystalline...multifunctional properties, such as dielectric, magnetic, optoelectronic, and magnetoelectric coupling behaviors. The control of organic crystallization and...electronics. In this project, we aim at utilizing the material design and assembly strategies to rationally develop organic multiferroic-photovoltaics

National Elevation Dataset

USGS Publications Warehouse

,

1999-01-01

The National Elevation Dataset (NED) is a new raster product assembled by the U.S. Geological Survey (USGS). The NED is designed to provide national elevation data in a seamless form with a consistent datum, elevation unit, and projection. Data corrections were made in the NED assembly process to minimize artifacts, permit edge matching, and fill sliver areas of missing data.

NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR PACKET ASSEMBLY, CUSTODY AND TRANSFER TO DATA SECTION (UA-C-3.0)

EPA Science Inventory

The purpose of this SOP is to outline the household (HH) packet assembly, custody, and transfer to the data section in preparation for data entry into the electronic database at the NHEXAS Arizona research project. This procedure was followed to ensure consistent data retrieval ...

Tailored Assembly of 2D Heterostructures beyond Graphene

DTIC Science & Technology

2017-05-11

liquid crystal and catalyst application. Another important approach we have explored during this project is the solution phase assembly of two...graphene oxide, and its potential functionalities in liquid crystal and catalyst application. Another important approach we have explored during...exfoliation, liquid phase exfoliation, and chemical vapor deposition, and opened up new opportunities to graphene based platform for novel

Decision support model for assessing archaeological survey needs for bridge replacement projects in Iowa.

DOT National Transportation Integrated Search

2006-01-01

The Bridges Decision Support Model is a geographic information system (GIS) that assembles existing : data on archaeological sites, surveys, and their geologic contexts to assess the risk of bridge replacement : projects encountering 13,000- to 150-y...

Looking Across Many Cancer Genomes - TCGA

Cancer.gov

TCGA's Pan-Cancer project publishes its integrative analysis of TCGA cancer types. The Pan-cancer project's goal is to assemble TCGA’s wealth of data across tumor types, analyze and interpret those data, and finally, make both the analyses and the data freely available.

Sequence verification of synthetic DNA by assembly of sequencing reads

PubMed Central

Wilson, Mandy L.; Cai, Yizhi; Hanlon, Regina; Taylor, Samantha; Chevreux, Bastien; Setubal, João C.; Tyler, Brett M.; Peccoud, Jean

2013-01-01

Gene synthesis attempts to assemble user-defined DNA sequences with base-level precision. Verifying the sequences of construction intermediates and the final product of a gene synthesis project is a critical part of the workflow, yet one that has received the least attention. Sequence validation is equally important for other kinds of curated clone collections. Ensuring that the physical sequence of a clone matches its published sequence is a common quality control step performed at least once over the course of a research project. GenoREAD is a web-based application that breaks the sequence verification process into two steps: the assembly of sequencing reads and the alignment of the resulting contig with a reference sequence. GenoREAD can determine if a clone matches its reference sequence. Its sophisticated reporting features help identify and troubleshoot problems that arise during the sequence verification process. GenoREAD has been experimentally validated on thousands of gene-sized constructs from an ORFeome project, and on longer sequences including whole plasmids and synthetic chromosomes. Comparing GenoREAD results with those from manual analysis of the sequencing data demonstrates that GenoREAD tends to be conservative in its diagnostic. GenoREAD is available at www.genoread.org. PMID:23042248

Mechanical feedback coordinates cell wall expansion and assembly in yeast mating morphogenesis

PubMed Central

2018-01-01

The shaping of individual cells requires a tight coordination of cell mechanics and growth. However, it is unclear how information about the mechanical state of the wall is relayed to the molecular processes building it, thereby enabling the coordination of cell wall expansion and assembly during morphogenesis. Combining theoretical and experimental approaches, we show that a mechanical feedback coordinating cell wall assembly and expansion is essential to sustain mating projection growth in budding yeast (Saccharomyces cerevisiae). Our theoretical results indicate that the mechanical feedback provided by the Cell Wall Integrity pathway, with cell wall stress sensors Wsc1 and Mid2 increasingly activating membrane-localized cell wall synthases Fks1/2 upon faster cell wall expansion, stabilizes mating projection growth without affecting cell shape. Experimental perturbation of the osmotic pressure and cell wall mechanics, as well as compromising the mechanical feedback through genetic deletion of the stress sensors, leads to cellular phenotypes that support the theoretical predictions. Our results indicate that while the existence of mechanical feedback is essential to stabilize mating projection growth, the shape and size of the cell are insensitive to the feedback. PMID:29346368

OSLay: optimal syntenic layout of unfinished assemblies.

PubMed

Richter, Daniel C; Schuster, Stephan C; Huson, Daniel H

2007-07-01

The whole genome shotgun approach to genome sequencing results in a collection of contigs that must be ordered and oriented to facilitate efficient gap closure. We present a new tool OSLay that uses synteny between matching sequences in a target assembly and a reference assembly to layout the contigs (or scaffolds) in the target assembly. The underlying algorithm is based on maximum weight matching. The tool provides an interactive visualization of the computed layout and the result can be imported into the assembly editing tool Consed to support the design of primer pairs for gap closure. To enhance efficiency in the gap closure phase of a genome project it is crucial to know which contigs are adjacent in the target genome. Related genome sequences can be used to layout contigs in an assembly. OSLay is freely available from: http://www-ab.informatik.unituebingen.de/software/oslay.

Solar Concentrator Advanced Development Program

NASA Technical Reports Server (NTRS)

Knasel, Don; Ehresman, Derik

1989-01-01

The Solar Concentrator Advanced Development Project has successfully designed, fabricated, and tested a full scale prototypical solar dynamic concentrator for space station applications. A Truss Hexagonal Panel reflector was selected as a viable solar concentrator concept to be used for space station applications. This concentrator utilizes a modular design approach and is flexible in attainable flux profiles and assembly techniques. The detailed design of the concentrator, which included structural, thermal and optical analysis, identified the feasibility of the design and specific technologies that were required to fabricate it. The needed surface accuracy of the reflectors surface was found to be very tight, within 5 mrad RMS slope error, and results in very close tolerances for fabrication. To meet the design requirements, a modular structure composed of hexagonal panels was used. The panels, made up of graphite epoxy box beams provided the strength, stiffness and dimensional stability needed. All initial project requirements were met or exceeded by hardware demonstration. Initial testing of structural repeatability of a seven panel portion of the concentrator was followed by assembly and testing of the full nineteen panel structure. The testing, which consisted of theodolite and optical measurements over an assembly-disassembly-reassembly cycle, demonstrated that the concentrator maintained the as-built contour and optical characteristics. The facet development effort within the project, which included developing the vapor deposited reflective facet, produced a viable design with demonstrated optical characteristics that are within the project goals.

Human Factors and Their Effects on Human-Centred Assembly Systems - A Literature Review-Based Study

NASA Astrophysics Data System (ADS)

Wang, Q.; Abubakar, M. I.

2017-09-01

If a product has more than one component, then it must be assembled. Assembly of products relies on assembly systems or lines in which assembly of each product is often carried out manually by human workers following assembly sequences in various forms. It is widely understood that efficiency of assembling a product by reducing assembly times (therefore costs) is vital particularly for small and medium-sized manufacturing companies to survive in an increasingly competitive market. Ideally, it is helpful for pre-determining efficiency or productivity of a human-centred assembly system at the early design stage. To date, most research on performance of an assembly system using modelling simulation methods is focused on its “operational functions”. The term used in a narrow sense always indicates the performance of the “operational system”, which does not incorporate the effect of human factors that may also affect the system performance. This paper presents a research outcome of findings through a literature review-based study by identifying possible human factors that mostly affect the performance on human-centred manufacturing systems as part of the research project incorporating parameters of human factors into a DES (discrete event simulation) tool.

National Ignition Facility Project: An Update

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hogan, W J; Moses, E; Warner, B

2000-12-07

The National Ignition Facility (NIF) consists of 192 forty-centimeter-square laser beams and a 10-m-diameter target chamber. Physical construction began in 1997. The Laser and Target Area Building and the Optics Assembly Building were the first major construction activities, and despite several unforeseen obstacles, the buildings are now 92% complete and have been done on time and within cost. Prototype component development and testing has proceeded in parallel. Optics vendors have installed full-scale production lines and have done prototype production runs. The assembly and integration of the beampath infrastructure has been reconsidered and a new approach has been developed. This papermore » will discuss the status of the NIF project and the plans for completion. It will also include summary information on Laser MegaJoule (LMJ) provided by M. Andre, LMJ Project Director.« less

The National Ignition Facility Project: An Update

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hogan, W.J.; Moses, E.; Warner, B.

2000-12-07

The National Ignition Facility (NIT) consists of 192 forty-centimeter-square laser beams and a 10-m-diameter target chamber. Physical construction began in 1997. The Laser and Target Area Building and the Optics Assembly Building were the first major construction activities, and despite several unforeseen obstacles, the buildings are now 92% complete and have been done on time and within cost. Prototype component development and testing has proceeded in parallel. Optics vendors have installed full-scale production lines and have done prototype production runs. The assembly and integration of the beam path infrastructure has been reconsidered and a new approach has been developed. Thismore » paper will discuss the status of the NIF project and the plans for completion. It will also include summary information on Laser MegaJoule (LMJ) provided by M. Andre, LMJ Project Director.« less

Analysis of Data in Accordance with Space Flight Mission Environmental Requirements

NASA Technical Reports Server (NTRS)

Shei, Monica

2011-01-01

The Environmental Assurance Program sets forth standards to ensure that all flight hardware is compatible with the environments that will be encountered during a spacecraft mission. It outlines the design, test and analysis, and risk control standards for the mission and certifies that it will survive in any external or self-induced environments that the spacecraft may experience. The Environmental Requirements Document (ERD) is the most important document in the Environmental Assurance Program, providing the design and test requirements for the project's flight system, subsystems, assemblies, and instruments. This summer's project was to assist Environmental Requirements Engineers (ERE's) in completing the Environmental Assurance Program Summary Report for both the Juno Project and Mars Science Laboratory (MSL) Project. The Summary Report is a document summarizing the environmental tests and analyses of each spacecraft at both the assembly and system level. It compiles a source of all relevant information such as waivers and Problem/Failure Reports (PFRs) into a single report for easy reference of how well the spacecraft met the requirements of the project.

Improving de novo sequence assembly using machine learning and comparative genomics for overlap correction.

PubMed

Palmer, Lance E; Dejori, Mathaeus; Bolanos, Randall; Fasulo, Daniel

2010-01-15

With the rapid expansion of DNA sequencing databases, it is now feasible to identify relevant information from prior sequencing projects and completed genomes and apply it to de novo sequencing of new organisms. As an example, this paper demonstrates how such extra information can be used to improve de novo assemblies by augmenting the overlapping step. Finding all pairs of overlapping reads is a key task in many genome assemblers, and to this end, highly efficient algorithms have been developed to find alignments in large collections of sequences. It is well known that due to repeated sequences, many aligned pairs of reads nevertheless do not overlap. But no overlapping algorithm to date takes a rigorous approach to separating aligned but non-overlapping read pairs from true overlaps. We present an approach that extends the Minimus assembler by a data driven step to classify overlaps as true or false prior to contig construction. We trained several different classification models within the Weka framework using various statistics derived from overlaps of reads available from prior sequencing projects. These statistics included percent mismatch and k-mer frequencies within the overlaps as well as a comparative genomics score derived from mapping reads to multiple reference genomes. We show that in real whole-genome sequencing data from the E. coli and S. aureus genomes, by providing a curated set of overlaps to the contigging phase of the assembler, we nearly doubled the median contig length (N50) without sacrificing coverage of the genome or increasing the number of mis-assemblies. Machine learning methods that use comparative and non-comparative features to classify overlaps as true or false can be used to improve the quality of a sequence assembly.

BAIT: Organizing genomes and mapping rearrangements in single cells.

PubMed

Hills, Mark; O'Neill, Kieran; Falconer, Ester; Brinkman, Ryan; Lansdorp, Peter M

2013-01-01

Strand-seq is a single-cell sequencing technique to finely map sister chromatid exchanges (SCEs) and other rearrangements. To analyze these data, we introduce BAIT, software which assigns templates and identifies and localizes SCEs. We demonstrate BAIT can refine completed reference assemblies, identifying approximately 21 Mb of incorrectly oriented fragments and placing over half (2.6 Mb) of the orphan fragments in mm10/GRCm38. BAIT also stratifies scaffold-stage assemblies, potentially accelerating the assembling and finishing of reference genomes. BAIT is available at http://sourceforge.net/projects/bait/.

Injection nozzle for a turbomachine

DOEpatents

Uhm, Jong Ho; Johnson, Thomas Edward; Kim, Kwanwoo

2012-09-11

A turbomachine includes a compressor, a combustor operatively connected to the compressor, an end cover mounted to the combustor, and an injection nozzle assembly operatively connected to the combustor. The injection nozzle assembly includes a first end portion that extends to a second end portion, and a plurality of tube elements provided at the second end portion. Each of the plurality of tube elements defining a fluid passage includes a body having a first end section that extends to a second end section. The second end section projects beyond the second end portion of the injection nozzle assembly.

Fiber Optic Cable Assemblies for Space Flight 2: Thermal and Radiation Effects

NASA Technical Reports Server (NTRS)

Ott, Melanie N.

1998-01-01

Goddard Space Flight Center is conducting a search for space flight worthy fiber optic cable assemblies that will benefit all projects at all of the NASA centers. This paper is number two in a series of papers being issued as a result of this task to define and qualify space grade fiber optic cable assemblies. Though to qualify and use a fiber optic cable in space requires treatment of the cable assembly as a system, it is very important to understand the design and behavior of its parts. This paper addresses that need, providing information on cable components shrinkage testing and radiation testing results from recent experiments at Goddard Space Flight Center.

Space Shuttle Projects

NASA Image and Video Library

1977-03-01

This photograph shows the liquid hydrogen tank and liquid oxygen tank for the Space Shuttle external tank (ET) being assembled in the weld assembly area of the Michoud Assembly Facility (MAF). The ET provides liquid hydrogen and liquid oxygen to the Shuttle's three main engines during the first eight 8.5 minutes of flight. At 154-feet long and more than 27-feet in diameter, the ET is the largest component of the Space Shuttle, the structural backbone of the entire Shuttle system, and the only part of the vehicle that is not reusable. The ET is manufactured at the Michoud Assembly Facility near New Orleans, Louisiana, by the Martin Marietta Corporation under management of the Marshall Space Flight Center.

A New Vision for the First Amendment in Schools.

ERIC Educational Resources Information Center

Chaltain, Sam

2002-01-01

Describes the First Amendment Schools project aimed at teaching K-12 public and independent school students their constitutionally protected religious, speech, press, assembly, and petition rights and responsibilities. Includes examples describing the project in several schools. Includes annotated list of resources for educators. (PKP)

Lightning Mapper Sensor Lens Assembly S.O. 5459: Project Management Plan

NASA Technical Reports Server (NTRS)

Zeidler, Janet

1999-01-01

Kaiser Electro-Optics, Inc. (KEO) has developed this Project Management Plan for the Lightning Mapper Sensor (LMS) program. KEO has integrated a team of experts in a structured program management organization to meet the needs of the LMS program. The project plan discusses KEO's approach to critical program elements including Program Management, Quality Assurance, Configuration Management, and Schedule.

Guidelines for developing distributed virtual environment applications

NASA Astrophysics Data System (ADS)

Stytz, Martin R.; Banks, Sheila B.

1998-08-01

We have conducted a variety of projects that served to investigate the limits of virtual environments and distributed virtual environment (DVE) technology for the military and medical professions. The projects include an application that allows the user to interactively explore a high-fidelity, dynamic scale model of the Solar System and a high-fidelity, photorealistic, rapidly reconfigurable aircraft simulator. Additional projects are a project for observing, analyzing, and understanding the activity in a military distributed virtual environment, a project to develop a distributed threat simulator for training Air Force pilots, a virtual spaceplane to determine user interface requirements for a planned military spaceplane system, and an automated wingman for use in supplementing or replacing human-controlled systems in a DVE. The last two projects are a virtual environment user interface framework; and a project for training hospital emergency department personnel. In the process of designing and assembling the DVE applications in support of these projects, we have developed rules of thumb and insights into assembling DVE applications and the environment itself. In this paper, we open with a brief review of the applications that were the source for our insights and then present the lessons learned as a result of these projects. The lessons we have learned fall primarily into five areas. These areas are requirements development, software architecture, human-computer interaction, graphical database modeling, and construction of computer-generated forces.

Effects of short read quality and quantity on a de novo vertebrate transcriptome assembly.

PubMed

Garcia, T I; Shen, Y; Catchen, J; Amores, A; Schartl, M; Postlethwait, J; Walter, R B

2012-01-01

For many researchers, next generation sequencing data holds the key to answering a category of questions previously unassailable. One of the important and challenging steps in achieving these goals is accurately assembling the massive quantity of short sequencing reads into full nucleic acid sequences. For research groups working with non-model or wild systems, short read assembly can pose a significant challenge due to the lack of pre-existing EST or genome reference libraries. While many publications describe the overall process of sequencing and assembly, few address the topic of how many and what types of reads are best for assembly. The goal of this project was use real world data to explore the effects of read quantity and short read quality scores on the resulting de novo assemblies. Using several samples of short reads of various sizes and qualities we produced many assemblies in an automated manner. We observe how the properties of read length, read quality, and read quantity affect the resulting assemblies and provide some general recommendations based on our real-world data set. Published by Elsevier Inc.

Xander: employing a novel method for efficient gene-targeted metagenomic assembly

DOE PAGES

Wang, Qiong; Fish, Jordan A.; Gilman, Mariah; ...

2015-08-05

Here, metagenomics can provide important insight into microbial communities. However, assembling metagenomic datasets has proven to be computationally challenging. Current methods often assemble only fragmented partial genes. We present a novel method for targeting assembly of specific protein-coding genes. This method combines a de Bruijn graph, as used in standard assembly approaches, and a protein profile hidden Markov model (HMM) for the gene of interest, as used in standard annotation approaches. These are used to create a novel combined weighted assembly graph. Xander performs both assembly and annotation concomitantly using information incorporated in this graph. We demonstrate the utility ofmore » this approach by assembling contigs for one phylogenetic marker gene and for two functional marker genes, first on Human Microbiome Project (HMP)-defined community Illumina data and then on 21 rhizosphere soil metagenomic datasets from three different crops totaling over 800 Gbp of unassembled data. We compared our method to a recently published bulk metagenome assembly method and a recently published gene-targeted assembler and found our method produced more, longer, and higher quality gene sequences. In conclusion, xander combines gene assignment with the rapid assembly of full-length or near full-length functional genes from metagenomic data without requiring bulk assembly or post-processing to find genes of interest. HMMs used for assembly can be tailored to the targeted genes, allowing flexibility to improve annotation over generic annotation pipelines.« less

A computational genomics pipeline for prokaryotic sequencing projects

PubMed Central

Kislyuk, Andrey O.; Katz, Lee S.; Agrawal, Sonia; Hagen, Matthew S.; Conley, Andrew B.; Jayaraman, Pushkala; Nelakuditi, Viswateja; Humphrey, Jay C.; Sammons, Scott A.; Govil, Dhwani; Mair, Raydel D.; Tatti, Kathleen M.; Tondella, Maria L.; Harcourt, Brian H.; Mayer, Leonard W.; Jordan, I. King

2010-01-01

Motivation: New sequencing technologies have accelerated research on prokaryotic genomes and have made genome sequencing operations outside major genome sequencing centers routine. However, no off-the-shelf solution exists for the combined assembly, gene prediction, genome annotation and data presentation necessary to interpret sequencing data. The resulting requirement to invest significant resources into custom informatics support for genome sequencing projects remains a major impediment to the accessibility of high-throughput sequence data. Results: We present a self-contained, automated high-throughput open source genome sequencing and computational genomics pipeline suitable for prokaryotic sequencing projects. The pipeline has been used at the Georgia Institute of Technology and the Centers for Disease Control and Prevention for the analysis of Neisseria meningitidis and Bordetella bronchiseptica genomes. The pipeline is capable of enhanced or manually assisted reference-based assembly using multiple assemblers and modes; gene predictor combining; and functional annotation of genes and gene products. Because every component of the pipeline is executed on a local machine with no need to access resources over the Internet, the pipeline is suitable for projects of a sensitive nature. Annotation of virulence-related features makes the pipeline particularly useful for projects working with pathogenic prokaryotes. Availability and implementation: The pipeline is licensed under the open-source GNU General Public License and available at the Georgia Tech Neisseria Base (http://nbase.biology.gatech.edu/). The pipeline is implemented with a combination of Perl, Bourne Shell and MySQL and is compatible with Linux and other Unix systems. Contact: king.jordan@biology.gatech.edu Supplementary information: Supplementary data are available at Bioinformatics online. PMID:20519285

NAC Off-Vehicle Brake Testing Project

DTIC Science & Technology

2007-05-01

disc pads/rotors and drum shoe assemblies/ drums - Must use vehicle “OEM” brake /hub-end hardware, or ESA... brake component comparison analysis (primary)* - brake system design analysis - brake system component failure analysis - (*) limited to disc pads...e.g. disc pads/rotors, drum shoe assemblies/ drums . - Not limited to “OEM” brake /hub-end hardware as there is none ! - Weight transfer, plumbing,

Extension Education: Training Coordinators to Facilitate Distance Education through the Assemblies of God Bible Institute in Belize

ERIC Educational Resources Information Center

Castleberry, Terry Lane

2010-01-01

The specific objective of this project was to train coordinators to open new extension classes and effectively facilitate existing extension classes offered by the Assemblies of God Bible Institute (AGBI) in Belize. Extensive biblical and literary research and a thorough evaluation of current extension classes led to the development of a…

Analysis of Advanced Fuel Assemblies and Core Designs for the Current and Next Generations of LWRs

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ragusa, Jean; Vierow, Karen

2011-09-01

The objective of the project is to design and analyze advanced fuel assemblies for use in current and future light water reactors and to assess their ability to reduce the inventory of transuranic elements, while preserving operational safety. The reprocessing of spent nuclear fuel can delay or avoid the need for a second geological repository in the US. Current light water reactor fuel assembly designs under investigation could reduce the plutonium inventory of reprocessed fuel. Nevertheless, these designs are not effective in stabilizing or reducing the inventory of minor actinides. In the course of this project, we developed and analyzedmore » advanced fuel assembly designs with improved thermal transmutation capability regarding transuranic elements and especially minor actinides. These designs will be intended for use in thermal spectrum (e.g., current and future fleet of light water reactors in the US). We investigated various fuel types, namely high burn-up advanced mixed oxides and inert matrix fuels, in various geometrical designs that are compliant with the core internals of current and future light water reactors. Neutronic/thermal hydraulic effects were included. Transmutation efficiency and safety parameters were used to rank and down-select the various designs.« less

Kodak Mirror Assembly Tested at Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

2003-01-01

The Eastman-Kodak mirror assembly is being tested for the James Webb Space Telescope (JWST) project at the X-Ray Calibration Facility at Marshall Space Flight Center (MSFC). In this photo, an MSFC employee is inspecting one of many segments of the mirror assembly for flaws. MSFC is supporting Goddard Space Flight Center (GSFC) in developing the JWST by taking numerous measurements to predict its future performance. The tests are conducted in a vacuum chamber cooled to approximate the super cold temperatures found in space. During its 27 years of operation, the facility has performed testing in support of a wide array of projects, including the Hubble Space Telescope (HST), Solar A, Chandra technology development, Chandra High Resolution Mirror Assembly and science instruments, Constellation X-Ray Mission, and Solar X-Ray Imager, currently operating on a Geostationary Operational Environment Satellite. The JWST is NASA's next generation space telescope, a successor to the Hubble Space Telescope, named in honor of NASA's second administrator, James E. Webb. It is scheduled for launch in 2010 aboard an expendable launch vehicle. It will take about 3 months for the spacecraft to reach its destination, an orbit of 940,000 miles in space.

Materials Processing in Space (MPS) program description

NASA Technical Reports Server (NTRS)

1981-01-01

Insight is provided into the scientific rotationale for materials processing in space (MPS), and a comprehensive and cohesive approach for implementation and integration of the many, diverse aspects of MPS is described. The programmatic and management functions apply to all projects and activities implemented under MPS. It is intended that specific project plans, providing project unique details, will be appended to this document for endeavors such as the Space Processing Applications Rocket (SPAR) Project, the Materials Experiment Assembly (MEA) Project, the MPS/Spacelab (MPS/SL) Project, and the Materials Experiment Carrier (MEC) Payloads.

Seven Liberal Outcomes of Professional Study.

ERIC Educational Resources Information Center

Stark, Joan S.

A group of faculty members and accrediting representatives was assembled to explore ways to better integrate liberal and professional study during the undergraduate years. The group based its discussions on educational outcomes previously identified by the project directors as important for all college-educated professionals. The project involved…

GFinisher: a new strategy to refine and finish bacterial genome assemblies

NASA Astrophysics Data System (ADS)

Guizelini, Dieval; Raittz, Roberto T.; Cruz, Leonardo M.; Souza, Emanuel M.; Steffens, Maria B. R.; Pedrosa, Fabio O.

2016-10-01

Despite the development in DNA sequencing technology, improving the number and the length of reads, the process of reconstruction of complete genome sequences, the so called genome assembly, is still complex. Only 13% of the prokaryotic genome sequencing projects have been completed. Draft genome sequences deposited in public databases are fragmented in contigs and may lack the full gene complement. The aim of the present work is to identify assembly errors and improve the assembly process of bacterial genomes. The biological patterns observed in genomic sequences and the application of a priori information can allow the identification of misassembled regions, and the reorganization and improvement of the overall de novo genome assembly. GFinisher starts generating a Fuzzy GC skew graphs for each contig in an assembly and follows breaking down the contigs in critical points in order to reassemble and close them using jFGap. This has been successfully applied to dataset from 96 genome assemblies, decreasing the number of contigs by up to 86%. GFinisher can easily optimize assemblies of prokaryotic draft genomes and can be used to improve the assembly programs based on nucleotide sequence patterns in the genome. The software and source code are available at http://gfinisher.sourceforge.net/.

GFinisher: a new strategy to refine and finish bacterial genome assemblies.

PubMed

Guizelini, Dieval; Raittz, Roberto T; Cruz, Leonardo M; Souza, Emanuel M; Steffens, Maria B R; Pedrosa, Fabio O

2016-10-10

Despite the development in DNA sequencing technology, improving the number and the length of reads, the process of reconstruction of complete genome sequences, the so called genome assembly, is still complex. Only 13% of the prokaryotic genome sequencing projects have been completed. Draft genome sequences deposited in public databases are fragmented in contigs and may lack the full gene complement. The aim of the present work is to identify assembly errors and improve the assembly process of bacterial genomes. The biological patterns observed in genomic sequences and the application of a priori information can allow the identification of misassembled regions, and the reorganization and improvement of the overall de novo genome assembly. GFinisher starts generating a Fuzzy GC skew graphs for each contig in an assembly and follows breaking down the contigs in critical points in order to reassemble and close them using jFGap. This has been successfully applied to dataset from 96 genome assemblies, decreasing the number of contigs by up to 86%. GFinisher can easily optimize assemblies of prokaryotic draft genomes and can be used to improve the assembly programs based on nucleotide sequence patterns in the genome. The software and source code are available at http://gfinisher.sourceforge.net/.

Californium interrogation prompt neutron (CIPN) instrument for non-destructive assay of spent nuclear fuel – design concept and experimental demonstration

DOE PAGES

Henzlova, Daniela; Menlove, Howard Olsen; Rael, Carlos D.; ...

2015-10-09

Our paper presents results of the first experimental demonstration of the Californium Interrogation Prompt Neutron (CIPN) instrument developed within a multi-year effort launched by the Next Generation Safeguards Initiative Spent Fuel Project of the United States Department of Energy. The goals of this project focused on developing viable non-destructive assay techniques with capabilities to improve an independent verification of spent fuel assembly characteristics. For this purpose, the CIPN instrument combines active and passive neutron interrogation, along with passive gamma-ray measurements, to provide three independent observables. We describe the initial feasibility demonstration of the CIPN instrument, which involved measurements of fourmore » pressurized-water-reactor spent fuel assemblies with different levels of burnup and two initial enrichments. The measurements were performed at the Post-Irradiation Examination Facility at the Korea Atomic Energy Institute in the Republic of Korea. The key aim of the demonstration was to evaluate CIPN instrument performance under realistic deployment conditions, with the focus on a detailed assessment of systematic uncertainties that are best evaluated experimentally. The measurements revealed good positioning reproducibility, as well as a high degree of insensitivity of the CIPN instrument's response to irregularities in a radial burnup profile. Systematic uncertainty of individual CIPN instrument signals due to assembly rotation was found to be <4.5%, even for assemblies with fairly extreme gradients in the radial burnup profile. Lastly, these features suggest that the CIPN instrument is capable of providing a good representation of assembly average characteristics, independent of assembly orientation in the instrument.« less

Californium interrogation prompt neutron (CIPN) instrument for non-destructive assay of spent nuclear fuel – design concept and experimental demonstration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Henzlova, Daniela; Menlove, Howard Olsen; Rael, Carlos D.

Our paper presents results of the first experimental demonstration of the Californium Interrogation Prompt Neutron (CIPN) instrument developed within a multi-year effort launched by the Next Generation Safeguards Initiative Spent Fuel Project of the United States Department of Energy. The goals of this project focused on developing viable non-destructive assay techniques with capabilities to improve an independent verification of spent fuel assembly characteristics. For this purpose, the CIPN instrument combines active and passive neutron interrogation, along with passive gamma-ray measurements, to provide three independent observables. We describe the initial feasibility demonstration of the CIPN instrument, which involved measurements of fourmore » pressurized-water-reactor spent fuel assemblies with different levels of burnup and two initial enrichments. The measurements were performed at the Post-Irradiation Examination Facility at the Korea Atomic Energy Institute in the Republic of Korea. The key aim of the demonstration was to evaluate CIPN instrument performance under realistic deployment conditions, with the focus on a detailed assessment of systematic uncertainties that are best evaluated experimentally. The measurements revealed good positioning reproducibility, as well as a high degree of insensitivity of the CIPN instrument's response to irregularities in a radial burnup profile. Systematic uncertainty of individual CIPN instrument signals due to assembly rotation was found to be <4.5%, even for assemblies with fairly extreme gradients in the radial burnup profile. Lastly, these features suggest that the CIPN instrument is capable of providing a good representation of assembly average characteristics, independent of assembly orientation in the instrument.« less

Xander: employing a novel method for efficient gene-targeted metagenomic assembly.

PubMed

Wang, Qiong; Fish, Jordan A; Gilman, Mariah; Sun, Yanni; Brown, C Titus; Tiedje, James M; Cole, James R

2015-01-01

Metagenomics can provide important insight into microbial communities. However, assembling metagenomic datasets has proven to be computationally challenging. Current methods often assemble only fragmented partial genes. We present a novel method for targeting assembly of specific protein-coding genes. This method combines a de Bruijn graph, as used in standard assembly approaches, and a protein profile hidden Markov model (HMM) for the gene of interest, as used in standard annotation approaches. These are used to create a novel combined weighted assembly graph. Xander performs both assembly and annotation concomitantly using information incorporated in this graph. We demonstrate the utility of this approach by assembling contigs for one phylogenetic marker gene and for two functional marker genes, first on Human Microbiome Project (HMP)-defined community Illumina data and then on 21 rhizosphere soil metagenomic datasets from three different crops totaling over 800 Gbp of unassembled data. We compared our method to a recently published bulk metagenome assembly method and a recently published gene-targeted assembler and found our method produced more, longer, and higher quality gene sequences. Xander combines gene assignment with the rapid assembly of full-length or near full-length functional genes from metagenomic data without requiring bulk assembly or post-processing to find genes of interest. HMMs used for assembly can be tailored to the targeted genes, allowing flexibility to improve annotation over generic annotation pipelines. This method is implemented as open source software and is available at https://github.com/rdpstaff/Xander_assembler.

Special ISO Class 6 Cleanroom for the Lunar Reconnaissance Orbiter (LRO) Project

NASA Technical Reports Server (NTRS)

Matthews, Richard A.; Matthews, Scott A.

2008-01-01

The parameters and restrictions for a horizontal flow ISO Class 6 Clean room to support the assembly of the new LRO (Lunar Reconnaissance Orbiter) were unusual. The project time line was critical. A novel Clean room design was developed and built within the time restraints. This paper describes the design criteria, timing, successful performance, and future benefits of this unique Clean room project.

Mars Science Laboratory Rover Taking Shape

NASA Technical Reports Server (NTRS)

2008-01-01

This image taken in August 2008 in a clean room at NASA's Jet Propulsion Laboratory, Pasadena, Calif., shows NASA's next Mars rover, the Mars Science Laboratory, in the course of its assembly, before additions of its arm, mast, laboratory instruments and other equipment.

The rover is about 9 feet wide and 10 feet long.

Viewing progress on the assembly are, from left: NASA Associate Administrator for Science Ed Weiler, California Institute of Technology President Jean-Lou Chameau, JPL Director Charles Elachi, and JPL Associate Director for Flight Projects and Mission Success Tom Gavin.

JPL, a division of Caltech, manages the Mars Science Laboratory project for the NASA Science Mission Directorate, Washington.

Use of Cumulative Degradation Factor Prediction and Life Test Result of the Thruster Gimbal Assembly Actuator for the Dawn Flight Project

NASA Technical Reports Server (NTRS)

Lo, C. John; Brophy, John R.; Etters, M. Andy; Ramesham, Rajeshuni; Jones, William R., Jr.; Jansen, Mark J.

2009-01-01

The Dawn Ion Propulsion System is the ninth project in NASA s Discovery Program. The Dawn spacecraft is being developed to enable the scientific investigation of the two heaviest main-belt asteroids, Vesta and Ceres. Dawn is the first mission to orbit two extraterrestrial bodies, and the first to orbit a main-belt asteroid. The mission is enabled by the onboard Ion Propulsion System (IPS) to provide the post-launch delta-V. The three Ion Engines of the IPS are mounted on Thruster Gimbal Assembly (TGA), with only one engine operating at a time for this 10-year mission. The three TGAs weigh 14.6 kg.

Solar power from satellites

NASA Technical Reports Server (NTRS)

Glaser, P. E.

1977-01-01

Microwave beaming of satellite-collected solar energy to earth for conversion to useful industrial power is evaluated for feasibility, with attention given to system efficiencies and costs, ecological impact, hardware to be employed, available options for energy conversion and transmission, and orbiting and assembly. Advantages of such a power generation and conversion system are listed, plausible techniques for conversion of solar energy (thermionic, thermal electric, photovoltaic) and transmission to earth (lasers, arrays of mirrors, microwave beams) are compared. Structural fatigue likely to result from brief daily eclipses, 55% system efficiency at the present state of the art, present projections of system costs, and projected economic implications of the technology are assessed. Two-stage orbiting and assembly plans are described.

Retraction Assembly for Space Shuttle Extended Nose Landing Gear

NASA Technical Reports Server (NTRS)

Files, Bradley S.; Nicholson, Leonard S. (Technical Monitor)

2000-01-01

As part of a project to encourage the use of shape memory alloy actuators for space actuators, this mechanism uses a nitinol ribbon to provide the necessary motion to help retract the proposed extended nose landing gear (ENLG) for the space shuttle. Initial proof-of-concept design of the ENLG did not include the ability to retract the gear automatically. One proposed actuator for this purpose was designed at Johnson Space Center and uses resistive heating to rotate the ribbon around a cylinder. This rotation then allows the assembly to pull down a wedge that is used to hold the landing gear strut in place, thus returning the landing gear to its previous height before extension. The presentation will follow the design of this assembly from working with the nitinol ribbon to providing mechanical connections and allowing minimal friction for motion of three wraps around a cylinder. Also to be presented is preliminary work on design of a shape memory alloy gripper, a design project to demonstrate uses of NiTi.

Research on construction quality and improvement of assembly construction

NASA Astrophysics Data System (ADS)

Cheng, Fei

2017-11-01

Based on the acceleration of the urbanization process and the improvement of the quality of life of our residents, the demand for building construction has been increasing. In this context, the construction industry in order to promote the construction efficiency, quality improvement, to meet the needs of the development of the times to strengthen the new technology, the use of new technologies. At present, China’s engineering construction units in the process of carrying out the project to strengthen the use of assembly-type construction technology, which thus achieved for the traditional construction work low-level, high time-consuming issues, and promote the steady improvement of production efficiency. Based on this, this paper focuses on the analysis of the connotation of the assembly structure and analyzes the quality problems in the construction process of the construction projects and puts forward the improvement measures to promote the improvement of the building quality and the construction of the building Construction speed. Based on this, this paper analyzes the structural system and design of prefabricated building.

Lifetime Estimation of a Time Projection Chamber X-ray Polarimeter

NASA Technical Reports Server (NTRS)

Hill, Joanne E.; Black, J. Kevin; Brieda, Lubos; Dickens, Patsy L.; deGarcia, Kristina Montt; Hawk, Douglas L.; Hayato, Asami; Jahoda, Keith; Mohammed, Jelila

2013-01-01

The Gravity and Extreme Magnetism Small Explorer (GEMS) X-ray polarimeter Instrument (XPI) was designed to measure the polarization of 23 sources over the course of its 9 month mission. The XPI design consists of two telescopes each with a polarimeter assembly at the focus of a grazing incidence mirror. To make sensitive polarization measurements the GEMS Polarimeter Assembly (PA) employed a gas detection system based on a Time Projection Chamber (TPC) technique. Gas detectors are inherently at risk of degraded performance arising from contamination from outgassing of internal detector components or due to loss of gas. This paper describes the design and the materials used to build a prototype of the flight polarimeter with the required GEMS lifetime. We report the results from outgassing measurements of the polarimeter subassemblies and assemblies, enclosure seal tests, life tests, and performance tests that demonstrate that the GEMS lifetime is achievable. Finally we report performance measurements and the lifetime enhancement from the use of a getter.

Classification of cryo electron microscopy images, noisy tomographic images recorded with unknown projection directions, by simultaneously estimating reconstructions and application to an assembly mutant of Cowpea Chlorotic Mottle Virus and portals of the bacteriophage P22

NASA Astrophysics Data System (ADS)

Lee, Junghoon; Zheng, Yili; Yin, Zhye; Doerschuk, Peter C.; Johnson, John E.

2010-08-01

Cryo electron microscopy is frequently used on biological specimens that show a mixture of different types of object. Because the electron beam rapidly destroys the specimen, the beam current is minimized which leads to noisy images (SNR substantially less than 1) and only one projection image per object (with an unknown projection direction) is collected. For situations where the objects can reasonably be described as coming from a finite set of classes, an approach based on joint maximum likelihood estimation of the reconstruction of each class and then use of the reconstructions to label the class of each image is described and demonstrated on two challenging problems: an assembly mutant of Cowpea Chlorotic Mottle Virus and portals of the bacteriophage P22.

Structural attachments for large space structures

NASA Technical Reports Server (NTRS)

Pruett, E. C.; Loughead, T. E.; Robertson, K. B., III

1980-01-01

The feasibility of fabricating beams in space and using them as components of a large, crew assembled structure, was investigated. Two projects were undertaken: (1) design and development of a ground version of an automated beam builder capable of producing triangular cross section aluminum beams; and (2) design and fabrication of lap joints to connect the beams orthogonally and centroidal end caps to connect beams end to end at any desired angle. The first project produced a beam building machine which fabricates aluminum beams suitable for neutral buoyancy evaluation. The second project produced concepts for the lap joint and end cap. However, neither of these joint concepts was suitable for use by a pressure suited crew member in a zero gravity environment. It is concluded that before the beams can be evaluated the joint designs need to be completed and sufficient joints produced to allow assembly of a complex structure.

Fuel nozzle assembly for use in turbine engines and methods of assembling same

DOEpatents

Uhm, Jong Ho; Johnson, Thomas Edward

2015-02-03

A fuel nozzle for use with a turbine engine is described herein. The fuel nozzle includes a housing that is coupled to a combustor liner defining a combustion chamber. The housing includes an endwall that at least partially defines the combustion chamber. A plurality of mixing tubes extends through the housing for channeling fuel to the combustion chamber. Each mixing tube of the plurality of mixing tubes includes an inner surface that extends between an inlet portion and an outlet portion. The outlet portion is oriented adjacent the housing endwall. At least one of the plurality of mixing tubes includes a plurality of projections that extend outwardly from the outlet portion. Adjacent projections are spaced a circumferential distance apart such that a groove is defined between each pair of circumferentially-apart projections to facilitate enhanced mixing of fuel in the combustion chamber.

Dual Telecentric Lens System For Projection Onto Tilted Toroidal Screen

NASA Technical Reports Server (NTRS)

Gold, Ronald S.; Hudyma, Russell M.

1995-01-01

System of two optical assemblies for projecting image onto tilted toroidal screen. One projection lens optimized for red and green spectral region; other for blue. Dual-channel approach offers several advantages which include: simplified color filtering, simplified chromatic aberration corrections, less complex polarizing prism arrangement, and increased throughput of blue light energy. Used in conjunction with any source of imagery, designed especially to project images formed by reflection of light from liquid-crystal light valve (LCLV).

What do Americans think about public transit? A review of U.S. public opinion polling survey questions.

DOT National Transportation Integrated Search

2015-07-01

This seed grant research project compiled a set of 56 US public opinion polls that asked respondents their opinions about public : transit. The first and primary goal of the project was to assemble a large set of transit-related survey question...

The Greening of a Brownfield: A Community-Based Learning Project in Economics

ERIC Educational Resources Information Center

Kaufman, Dennis A.; Cloutier, Norman R.

2004-01-01

This paper describes a community-based learning (CBL) project involving the economic redevelopment of two environmentally contaminated former industrial sites or "brownfields." The study was conducted over four semesters by several economics classes and involved assembling a database, measuring the impact of the brownfields on…

An Interdisciplinary Microprocessor Project.

ERIC Educational Resources Information Center

Wilcox, Alan D.; And Others

1985-01-01

Describes an unusual project in which third-year computer science students designed and built a four-bit multiplier circuit and then combines it with software to complete a full 16-bit multiplication. The multiplier was built using TTL components, interfaced with a Z-80 microprocessor system, and programed in assembly language. (JN)

The Minnesota Defense Industry Conversion Project. A Partnership for Retraining.

ERIC Educational Resources Information Center

Daines, James R.; And Others

The Minnesota Defense Conversion Adjustment Project was initiated in 1993 with funding provided through the U.S. Department of Labor's Defense Conversion Adjustment Program to help workers at a Minnesota defense plant make the transition from assembler and related production classifications to machinists and other positions requiring specific job…

Hypersonic research engine project. Phase 2: Aerothermodynamic integration model development

NASA Technical Reports Server (NTRS)

Jilly, L. F. (Editor)

1970-01-01

The analytical effort was directed towards (1) completing the design of the combustor exit instrumentation assembly, (2) analyzing the coolant flow distribution of the cowl leading edge tip section, (3) determining effects of purge gas pressure on AIM performance analysis, and (4) analyzing heat transfer and associated stress problems related to the cowl leading edge tip section and the nozzle shroud assembly for test conditions.

Face seal assembly for rotating drum

DOEpatents

Morgan, J. Giles; Rennich, Mark J.; Whatley, Marvin E.

1982-01-01

A seal assembly comprises a tube rotatable about its longitudinal axis and having two longitudinally spaced flanges projecting radially outwardly from the outer surface thereof. Slidably positioned against one of the flanges is a seal ring, and disposed between this seal ring and the other flange are two rings that are forced apart by springs, one of the latter rings being attached to a flexible wall.

Calculated Drag of an Aerial Refueling Assembly Through Airplane Performance Analysis

NASA Technical Reports Server (NTRS)

Vachon, Jake; Ray, Ronald; Calianno, Carl

2004-01-01

This viewgraph document reviews NASA Dryden's work on Aerial refueling, with specific interest in calculating the drag of the refueling system. The aerodynamic drag of an aerial refueling assembly was calculated during the Automated Aerial Refueling project at the NASA Dryden Flight Research Center. An F/A-18A airplane was specially instrumented to obtain accurate fuel flow measurements and to determine engine thrust

National Elevation Dataset

USGS Publications Warehouse

,

2002-01-01

The National Elevation Dataset (NED) is a new raster product assembled by the U.S. Geological Survey. NED is designed to provide National elevation data in a seamless form with a consistent datum, elevation unit, and projection. Data corrections were made in the NED assembly process to minimize artifacts, perform edge matching, and fill sliver areas of missing data. NED has a resolution of one arc-second (approximately 30 meters) for the conterminous United States, Hawaii, Puerto Rico and the island territories and a resolution of two arc-seconds for Alaska. NED data sources have a variety of elevation units, horizontal datums, and map projections. In the NED assembly process the elevation values are converted to decimal meters as a consistent unit of measure, NAD83 is consistently used as horizontal datum, and all the data are recast in a geographic projection. Older DEM's produced by methods that are now obsolete have been filtered during the NED assembly process to minimize artifacts that are commonly found in data produced by these methods. Artifact removal greatly improves the quality of the slope, shaded-relief, and synthetic drainage information that can be derived from the elevation data. Figure 2 illustrates the results of this artifact removal filtering. NED processing also includes steps to adjust values where adjacent DEM's do not match well, and to fill sliver areas of missing data between DEM's. These processing steps ensure that NED has no void areas and artificial discontinuities have been minimized. The artifact removal filtering process does not eliminate all of the artifacts. In areas where the only available DEM is produced by older methods, then "striping" may still occur.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Montgomery, Rose; Scaglione, John M; Bevard, Bruce Balkcom

The High Burnup Spent Fuel Data project pulled 25 sister rods (9 from the project assemblies and 16 from similar HBU assemblies) for characterization. The 25 sister rods are all high burnup and cover the range of modern domestic cladding alloys. The 25 sister rods were shipped to Oak Ridge National Laboratory (ORNL) in early 2016 for detailed non-destructive and destructive examination. Examinations are intended to provide baseline data on the initial physical state of the cladding and fuel prior to the loading, drying, and long-term dry storage process. Further examinations are focused on determining the effects of temperatures encounteredmore » during and following drying. Similar tests will be performed on rods taken from the project assemblies at the end of their long-term storage in a TN-32 dry storage cask (the cask rods ) to identify any significant changes in the fuel rods that may have occurred during the dry storage period. Additionally, some of the sister rods will be used for separate effects testing to expand the applicability of the project data to the fleet, and to address some of the data-related gaps associated with extended storage and subsequent transportation of high burnup fuel. A draft test plan is being developed that describes the experimental work to be conducted on the sister rods. This paper summarizes the draft test plan and necessary coordination activities for the multi-year experimental program to supply data relevant to the assessment of the safety of long-term storage followed by transportation of high burnup spent fuel.« less

Project Mercury; Little Joe

NASA Image and Video Library

1959-07-30

Assembling the Little Joe capsules. The capsules were manufactured in-house by Langley technicians. Three capsules are shown here in various stages of assembly. The escape tower and rocket motors shown on the completed capsule would be removed before shipping and finally assembly for launching at Wallops Island. Joseph Shortal wrote (vol. 3, p. 32): Design of the Little Joe capsules began at Langley before McDonnell started on the design of the Mercury capsule and was, therefore, a separate design. Although it was not designed to carry a man, it did have to carry a monkey. It had to meet the weight and center of gravity requirements of Mercury and withstand the same aerodynamic loads during the exit trajectory. Although in comparison with the overall Mercury Project, Little Joe was a simple undertaking, the fact that an attempt was made to condense a normal two-year project into a 6-month one with in house labor turned it into a major undertaking for Langley. Project Mercury: Little Joe: Boilerplate Mercury spacecraft undergo fabrication at the shops of the Langley Research Center. They will launched atop Little Joe rockets to test the spacecraft recovery systems. -- Published in Joseph A. Shortal, History of Wallops Station: Origins and Activities Through 1949, (Wallops Island, VA: National Aeronautics and Space Administration, Wallops Station, nd), Comment Edition. L59-4947 Technicians prepare a Little Joe launch vehicle prototype for the Mercury space program, 1959. Photograph published in Winds of Change, 75th Anniversary NASA publication, page 76, by James Schultz

Nuclear reactor power for a space-based radar. SP-100 project

NASA Technical Reports Server (NTRS)

Bloomfield, Harvey; Heller, Jack; Jaffe, Leonard; Beatty, Richard; Bhandari, Pradeep; Chow, Edwin; Deininger, William; Ewell, Richard; Fujita, Toshio; Grossman, Merlin

1986-01-01

A space-based radar mission and spacecraft, using a 300 kWe nuclear reactor power system, has been examined, with emphasis on aspects affecting the power system. The radar antenna is a horizontal planar array, 32 X 64 m. The orbit is at 61 deg, 1088 km. The mass of the antenna with support structure is 42,000 kg; of the nuclear reactor power system, 8,300 kg; of the whole spacecraft about 51,000 kg, necessitating multiple launches and orbital assembly. The assembly orbit is at 57 deg, 400 km, high enough to provide the orbital lifetime needed for orbital assembly. The selected scenario uses six Shuttle launches to bring the spacecraft and a Centaur G upper-stage vehicle to assembly orbit. After assembly, the Centaur places the spacecraft in operational orbit, where it is deployed on radio command, the power system started, and the spacecraft becomes operational. Electric propulsion is an alternative and allows deployment in assembly orbit, but introduces a question of nuclear safety.

Photo-reduction on the rupture of disulfide bonds and the related protein assembling

NASA Astrophysics Data System (ADS)

Wang, Wei

It has been found that many proteins can self-assemble into nanoscale assemblies when they unfold or partially unfold under harsh conditions, such as low pH, high temperature, or the presence of denaturants, and so on. These nanoscale assemblies can have some applications such as the drug-delivery systems (DDSs). Here we report a study that a very physical way, the UV illumination, can be used to facilitate the formation of protein fibrils and nanoparticles under native conditions by breaking disulfide bonds in some disulfide-containing proteins. By controlling the intensity of UV light and the illumination time, we realized the preparation of self-assembly nanoparticles which encapsulate the anticancer drug doxorubicin (DOX) and can be used as the DDS for inhibiting the growth of tumor. The formation of fibrillary assemblies was also observed. The rupture of disulfide bonds through photo-reduction process due to the effect of tryptophan and tyrosine was studied, and the physical mechanism of the assembling of the related disulfide-containing proteins was also discussed. We thank the financial support from NSF of China and the 973 project.

America's Next Great Ship: Space Launch System Core Stage Transitioning from Design to Manufacturing

NASA Technical Reports Server (NTRS)

Birkenstock, Benjamin; Kauer, Roy

2014-01-01

The Space Launch System (SLS) Program is essential to achieving the Nation's and NASA's goal of human exploration and scientific investigation of the solar system. As a multi-element program with emphasis on safety, affordability, and sustainability, SLS is becoming America's next great ship of exploration. The SLS Core Stage includes avionics, main propulsion system, pressure vessels, thrust vector control, and structures. Boeing manufactures and assembles the SLS core stage at the Michoud Assembly Facility (MAF) in New Orleans, LA, a historical production center for Saturn V and Space Shuttle programs. As the transition from design to manufacturing progresses, the importance of a well-executed manufacturing, assembly, and operation (MA&O) plan is crucial to meeting performance objectives. Boeing employs classic techniques such as critical path analysis and facility requirements definition as well as innovative approaches such as Constraint Based Scheduling (CBS) and Cirtical Chain Project Management (CCPM) theory to provide a comprehensive suite of project management tools to manage the health of the baseline plan on both a macro (overall project) and micro level (factory areas). These tools coordinate data from multiple business systems and provide a robust network to support Material & Capacity Requirements Planning (MRP/CRP) and priorities. Coupled with these tools and a highly skilled workforce, Boeing is orchestrating the parallel buildup of five major sub assemblies throughout the factory. Boeing and NASA are transforming MAF to host state of the art processes, equipment and tooling, the most prominent of which is the Vertical Assembly Center (VAC), the largest weld tool in the world. In concert, a global supply chain is delivering a range of structural elements and component parts necessary to enable an on-time delivery of the integrated Core Stage. SLS is on plan to launch humanity into the next phase of space exploration.

Teslaphoresis of Carbon Nanotubes.

PubMed

Bornhoeft, Lindsey R; Castillo, Aida C; Smalley, Preston R; Kittrell, Carter; James, Dustin K; Brinson, Bruce E; Rybolt, Thomas R; Johnson, Bruce R; Cherukuri, Tonya K; Cherukuri, Paul

2016-04-26

This paper introduces Teslaphoresis, the directed motion and self-assembly of matter by a Tesla coil, and studies this electrokinetic phenomenon using single-walled carbon nanotubes (CNTs). Conventional directed self-assembly of matter using electric fields has been restricted to small scale structures, but with Teslaphoresis, we exceed this limitation by using the Tesla coil's antenna to create a gradient high-voltage force field that projects into free space. CNTs placed within the Teslaphoretic (TEP) field polarize and self-assemble into wires that span from the nanoscale to the macroscale, the longest thus far being 15 cm. We show that the TEP field not only directs the self-assembly of long nanotube wires at remote distances (>30 cm) but can also wirelessly power nanotube-based LED circuits. Furthermore, individualized CNTs self-organize to form long parallel arrays with high fidelity alignment to the TEP field. Thus, Teslaphoresis is effective for directed self-assembly from the bottom-up to the macroscale.

Analysis of space environment damage to solar cell assemblies from LDEF experiment A0171-GSFC test plate

NASA Technical Reports Server (NTRS)

Hill, David C.; Rose, M. Frank

1994-01-01

The results of the postflight analysis of the solar cell assemblies from the LDEF (Long Duration Exposure facility) experiment A0171 is provided in this NASA sponsored research project. The following data on this research are provided as follows: (1) solar cell description, including, substrate composition and thickness, crystal orientation, anti-reflective coating composition and thickness; (2) preflight characteristics of the solar cell assemblies with respect to current and voltage; and (3) post-flight characteristics of the solar cell assemblies with respect to voltage and current. These solar cell assemblies are part of the Goddard Space Flight Center test plate which was designed to test the space environment effects (radiation, atomic oxygen, thermal cycling, meteoroid and debris) on conductively coated solar cell coversheets, various electrical bond materials, solar cell performance, and other material properties where feasible.

Top Ten Tips for Assessing Project-Based Learning: Plus, a Bonus Tip on How to Assemble a PBL Tool Kit

ERIC Educational Resources Information Center

George Lucas Educational Foundation, 2011

2011-01-01

This classroom guide is intended to inspire and expand teachers' thinking about effective assessment in project-based learning (PBL). The tips listed in this guide are organized to follow the arc of a project. First comes planning, then the launch into active learning, and then a culminating presentation. Reflection is the final stage, and it's…

Atomistic Design and Simulations of Nanoscale Machines and Assembly

NASA Technical Reports Server (NTRS)

Goddard, William A., III; Cagin, Tahir; Walch, Stephen P.

2000-01-01

Over the three years of this project, we made significant progress on critical theoretical and computational issues in nanoscale science and technology, particularly in:(1) Fullerenes and nanotubes, (2) Characterization of surfaces of diamond and silicon for NEMS applications, (3) Nanoscale machine and assemblies, (4) Organic nanostructures and dendrimers, (5) Nanoscale confinement and nanotribology, (6) Dynamic response of nanoscale structures nanowires (metals, tubes, fullerenes), (7) Thermal transport in nanostructures.

Effect of thermal cycling ramp rate on CSP assembly reliability

NASA Technical Reports Server (NTRS)

Ghaffarian, R.

2001-01-01

A JPL-led chip scale package consortium of enterprises recently joined together to pool in-kind resources for developing the quality and reliability of chip scale packages for a variety of projects. The experience of the consortium in building more than 150 test vehicle assemblies, single and double sided multilayer PWBs, and the environmental test results has now been published as a chip scale package guidelines document.

Critical Issues Facing America's Community Colleges: A Summary of the Community College Futures Assembly 2011 Mixed Methods/Appreciative Inquiry Research Project

ERIC Educational Resources Information Center

Basham, Matthew J.; Campbell, Dale F.; Mahmood, Hajara; Martin, Kenyatta

2012-01-01

For almost 20 years the Community College Futures Assembly (CCFA) has met annually in Orlando, Florida to serve as a showcase of best practices in community college administration and to serve as a think-tank for research and policy. Through the years the research methodology has evolved. The 2011 CCFA used a mixed-methods approach: qualitative…

Modeling the self-assembly of ordered nanoporous materials

DOE Office of Scientific and Technical Information (OSTI.GOV)

Monson, Peter; Auerbach, Scott

This report describes progress on a collaborative project on the multiscale modeling of the assembly processes in the synthesis of nanoporous materials. Such materials are of enormous importance in modern technology with application in the chemical process industries, biomedicine and biotechnology as well as microelectronics. The project focuses on two important classes of materials: i) microporous crystalline materials, such as zeolites, and ii) ordered mesoporous materials. In the first case the pores are part of the crystalline structure, while in the second the structures are amorphous on the atomistic length scale but where surfactant templating gives rise to order onmore » the length scale of 2 - 20 nm. We have developed a modeling framework that encompasses both these kinds of materials. Our models focus on the assembly of corner sharing silica tetrahedra in the presence of structure directing agents. We emphasize a balance between sufficient realism in the models and computational tractibility given the complex many-body phenomena. We use both on-lattice and off-lattice models and the primary computational tools are Monte Carlo simulations with sampling techniques and ensembles appropriate to specific situations. Our modeling approach is the first to capture silica polymerization, nanopore crystallization, and mesopore formation through computer-simulated self assembly.« less

Spherical primary optical telescope (SPOT) segments

NASA Astrophysics Data System (ADS)

Hall, Christopher; Hagopian, John; DeMarco, Michael

2012-09-01

The spherical primary optical telescope (SPOT) project is an internal research and development program at NASA Goddard Space Flight Center. The goals of the program are to develop a robust and cost effective way to manufacture spherical mirror segments and demonstrate a new wavefront sensing approach for continuous phasing across the segmented primary. This paper focuses on the fabrication of the mirror segments. Significant cost savings were achieved through the design, since it allowed the mirror segments to be cast rather than machined from a glass blank. Casting was followed by conventional figuring at Goddard Space Flight Center. After polishing, the mirror segments were mounted to their composite assemblies. QED Technologies used magnetorheological finishing (MRF®) for the final figuring. The MRF process polished the mirrors while they were mounted to their composite assemblies. Each assembly included several magnetic invar plugs that extended to within an inch of the face of the mirror. As part of this project, the interaction between the MRF magnetic field and invar plugs was evaluated. By properly selecting the polishing conditions, MRF was able to significantly improve the figure of the mounted segments. The final MRF figuring demonstrates that mirrors, in the mounted configuration, can be polished and tested to specification. There are significant process capability advantes due to polishing and testing the optics in their final, end-use assembled state.

Kodak Mirror Assembly Tested at Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

2003-01-01

The Eastman-Kodak mirror assembly is being tested for the James Webb Space Telescope (JWST) project at the X-Ray Calibration Facility at Marshall Space Flight Center (MSFC). In this photo, one of many segments of the mirror assembly is being set up inside the 24-ft vacuum chamber where it will undergo x-ray calibration tests. MSFC is supporting Goddard Space Flight Center (GSFC) in developing the JWST by taking numerous measurements to predict its future performance. The tests are conducted in a vacuum chamber cooled to approximate the super cold temperatures found in space. During its 27 years of operation, the facility has performed testing in support of a wide array of projects, including the Hubble Space Telescope (HST), Solar A, Chandra technology development, Chandra High Resolution Mirror Assembly and science instruments, Constellation X-Ray Mission, and Solar X-Ray Imager, currently operating on a Geostationary Operational Environment Satellite. The JWST is NASA's next generation space telescope, a successor to the Hubble Space Telescope, named in honor of NASA's second administrator, James E. Webb. It is scheduled for launch in 2010 aboard an expendable launch vehicle. It will take about 3 months for the spacecraft to reach its destination, an orbit of 940,000 miles in space.

Precision lens assembly with alignment turning system

NASA Astrophysics Data System (ADS)

Ho, Cheng-Fang; Huang, Chien-Yao; Lin, Yi-Hao; Kuo, Hui-Jean; Kuo, Ching-Hsiang; Hsu, Wei-Yao; Chen, Fong-Zhi

2017-10-01

The poker chip assembly with high precision lens barrels is widely applied to ultra-high performance optical system. ITRC applies the poker chip assembly technology to the high numerical aperture objective lenses and lithography projection lenses because of its high efficiency assembly process. In order to achieve high precision lens cell for poker chip assembly, an alignment turning system (ATS) is developed. The ATS includes measurement, alignment and turning modules. The measurement module is equipped with a non-contact displacement sensor (NCDS) and an autocollimator (ACM). The NCDS and ACM are used to measure centration errors of the top and the bottom surface of a lens respectively; then the amount of adjustment of displacement and tilt with respect to the rotational axis of the turning machine for the alignment module can be determined. After measurement, alignment and turning processes on the ATS, the centration error of a lens cell with 200 mm in diameter can be controlled within 10 arcsec. Furthermore, a poker chip assembly lens cell with three sub-cells is demonstrated, each sub-cells are measured and accomplished with alignment and turning processes. The lens assembly test for five times by each three technicians; the average transmission centration error of assembly lens is 12.45 arcsec. The results show that ATS can achieve high assembly efficiency for precision optical systems.

Chemistry-Materials Laboratory Project Book, 1979-80.

ERIC Educational Resources Information Center

Connecticut State Dept. of Education, Hartford. Bureau of Vocational-Technical Schools.

This Chemistry-Materials Laboratory Project Book, assembled through a survey of science instructors in vocational-technical schools in Connecticut, is intended to meet a variety of needs. It can serve as an idea book, with the instructor taking from it as needed and adding or substituting material related to class interests; as a guide book for…

Project WISE: Building STEM-Focused Youth-Programs that Serve the Community

ERIC Educational Resources Information Center

DiLisi, Gregory A.; McMillin, Keith A.; Virostek, Margaret E.

2011-01-01

We describe the design and implementation of Project WISE, a multi-institutional partnership that assembles interdisciplinary teams of undergraduate and high school students charged with developing STEM-focused community youth-programs. Our goal is twofold: (i.) to promote young women's interest in STEM-oriented careers through an early, positive…

Project Hill-Climb: Drafting and Design in Motion

ERIC Educational Resources Information Center

Crowl, William F.

2008-01-01

This article describes the Hill-Climb project of a second level Computer-Aided Drafting and Design (CADD) class. The author primarily designed the activity to increase student understanding of the assembly drawing process and its components. The emphasis on problem solving adds a dimension that can aid students in their other classes as well. By…

Meeting the challenges of non-referenced genome assembly from short-read sequence data

Treesearch

M. Parks; A. Liston; R. Cronn

2010-01-01

Massively parallel sequencing technologies (MPST) offer unprecedented opportunities for novel sequencing projects. MPST, while offering tremendous sequencing capacity, are typically most effective in resequencing projects (as opposed to the sequencing of novel genomes) due to the fact that sequence is returned in relatively short reads. Nonetheless, there is great...

The Advanced Interdisciplinary Research Laboratory: A Student Team Approach to the Fourth-Year Research Thesis Project Experience

ERIC Educational Resources Information Center

Piunno, Paul A. E.; Boyd, Cleo; Barzda, Virginijus; Gradinaru, Claudiu C.; Krull, Ulrich J.; Stefanovic, Sasa; Stewart, Bryan

2014-01-01

The advanced interdisciplinary research laboratory (AIRLab) represents a novel, effective, and motivational course designed from the interdisciplinary research interests of chemistry, physics, biology, and education development faculty members as an alternative to the independent thesis project experience. Student teams are assembled to work…

Independent Peer Review of Communications, Navigation, and Networking re-Configurable Testbed (CoNNeCT) Project Antenna Pointing Subsystem (APS) Integrated Gimbal Assembly (IGA) Structural Analysis

NASA Technical Reports Server (NTRS)

Raju, Ivatury S.; Larsen, Curtis E.; Pellicciotti, Joseph W.

2010-01-01

Glenn Research Center Chief Engineer's Office requested an independent review of the structural analysis and modeling of the Communications, Navigation, and Networking re-Configurable Testbed (CoNNeCT) Project Antenna Pointing Subsystem (APS) Integrated Gimbal Assembly (IGA) to be conducted by the NASA Engineering and Safety Center (NESC). At this time, the IGA had completed its critical design review (CDR). The assessment was to be a peer review of the NEi-NASTRAN1 model of the APS Antenna, and not a peer review of the design and the analysis that had been completed by the GRC team for CDR. Thus, only a limited amount of information was provided on the structural analysis. However, the NESC team had difficulty separating analysis concerns from modeling issues. The team studied the NASTRAN model, but did not fully investigate how the model was used by the CoNNeCT Project and how the Project was interpreting the results. The team's findings, observations, and NESC recommendations are contained in this report.

The Mouse Genomes Project: a repository of inbred laboratory mouse strain genomes.

PubMed

Adams, David J; Doran, Anthony G; Lilue, Jingtao; Keane, Thomas M

2015-10-01

The Mouse Genomes Project was initiated in 2009 with the goal of using next-generation sequencing technologies to catalogue molecular variation in the common laboratory mouse strains, and a selected set of wild-derived inbred strains. The initial sequencing and survey of sequence variation in 17 inbred strains was completed in 2011 and included comprehensive catalogue of single nucleotide polymorphisms, short insertion/deletions, larger structural variants including their fine scale architecture and landscape of transposable element variation, and genomic sites subject to post-transcriptional alteration of RNA. From this beginning, the resource has expanded significantly to include 36 fully sequenced inbred laboratory mouse strains, a refined and updated data processing pipeline, and new variation querying and data visualisation tools which are available on the project's website ( http://www.sanger.ac.uk/resources/mouse/genomes/ ). The focus of the project is now the completion of de novo assembled chromosome sequences and strain-specific gene structures for the core strains. We discuss how the assembled chromosomes will power comparative analysis, data access tools and future directions of mouse genetics.

Telescope Scientist on the Advanced X-Ray Astrophysics Observatory

NASA Technical Reports Server (NTRS)

VanSpeybroeck, Leon

1999-01-01

The most important activity during this reporting period was the calibration of the AXAF High Resolution Mirror Assembly (HRMA) and the analysis of the copious data which were obtained during that project. The calibration was highly successful, and will result in the AXAF being by far the best calibrated X-ray observatory ever flown, and more accurate results by all of its users. This period also included participation in the spacecraft alignment and assembly activities and final flight readiness reviews. The planning of the first year of Telescope Scientist AXAF observations also was accomplished. The Telescope Scientist team also served as a technical resource for various problems which were encountered during this period. Many of these contributions have been documented in memoranda sent to the project.

Photovoltaic assemblies and methods for transporting

DOEpatents

Almy, Charles; Campbell, Matt; Sandler, Reuben; Wares, Brian; Wayman, Elizabeth

2014-08-05

A PV assembly including framework, PV laminate(s), and a stiffening device. The framework includes a perimeter frame at least 10 feet in length and at least 5 feet in width. The PV laminate(s) are assembled to the perimeter frame to define a receiving zone having a depth of not more than 8 inches. The stiffening device is associated with the framework and is configured to provide a first state and a second state. In the first state, an entirety of the stiffening device is maintained within the receiving zone. In the second state, at least a portion of the stiffening device projects from the receiving zone. The stiffening device enhances a stiffness of the PV assembly in a plane of the perimeter frame, and can include rods defining truss structures.

Y and W Chromosome Assemblies: Approaches and Discoveries.

PubMed

Tomaszkiewicz, Marta; Medvedev, Paul; Makova, Kateryna D

2017-04-01

Hundreds of vertebrate genomes have been sequenced and assembled to date. However, most sequencing projects have ignored the sex chromosomes unique to the heterogametic sex - Y and W - that are known as sex-limited chromosomes (SLCs). Indeed, haploid and repetitive Y chromosomes in species with male heterogamety (XY), and W chromosomes in species with female heterogamety (ZW), are difficult to sequence and assemble. Nevertheless, obtaining their sequences is important for understanding the intricacies of vertebrate genome function and evolution. Recent progress has been made towards the adaptation of next-generation sequencing (NGS) techniques to deciphering SLC sequences. We review here currently available methodology and results with regard to SLC sequencing and assembly. We focus on vertebrates, but bring in some examples from other taxa. Copyright © 2017 Elsevier Ltd. All rights reserved.

Photovoltaic assemblies and methods for transporting

DOEpatents

Almy, Charles; Campbell, Matt; Sandler, Reuben; Wares, Brian; Wayman, Elizabeth

2013-09-17

A PV assembly including framework, PV laminate(s), and a stiffening device. The framework includes a perimeter frame at least 10 feet in length and at least 5 feet in width. The PV laminate(s) are assembled to the perimeter frame to define a receiving zone having a depth of not more than 8 inches. The stiffening device is associated with the framework and is configured to provide a first state and a second state. In the first state, an entirety of the stiffening device is maintained within the receiving zone. In the second state, at least a portion of the stiffening device projects from the receiving zone. The stiffening device enhances a stiffness of the PV assembly in a plane of the perimeter frame, and can include rods defining truss structures.

LightAssembler: fast and memory-efficient assembly algorithm for high-throughput sequencing reads.

PubMed

El-Metwally, Sara; Zakaria, Magdi; Hamza, Taher

2016-11-01

The deluge of current sequenced data has exceeded Moore's Law, more than doubling every 2 years since the next-generation sequencing (NGS) technologies were invented. Accordingly, we will able to generate more and more data with high speed at fixed cost, but lack the computational resources to store, process and analyze it. With error prone high throughput NGS reads and genomic repeats, the assembly graph contains massive amount of redundant nodes and branching edges. Most assembly pipelines require this large graph to reside in memory to start their workflows, which is intractable for mammalian genomes. Resource-efficient genome assemblers combine both the power of advanced computing techniques and innovative data structures to encode the assembly graph efficiently in a computer memory. LightAssembler is a lightweight assembly algorithm designed to be executed on a desktop machine. It uses a pair of cache oblivious Bloom filters, one holding a uniform sample of [Formula: see text]-spaced sequenced [Formula: see text]-mers and the other holding [Formula: see text]-mers classified as likely correct, using a simple statistical test. LightAssembler contains a light implementation of the graph traversal and simplification modules that achieves comparable assembly accuracy and contiguity to other competing tools. Our method reduces the memory usage by [Formula: see text] compared to the resource-efficient assemblers using benchmark datasets from GAGE and Assemblathon projects. While LightAssembler can be considered as a gap-based sequence assembler, different gap sizes result in an almost constant assembly size and genome coverage. https://github.com/SaraEl-Metwally/LightAssembler CONTACT: sarah_almetwally4@mans.edu.egSupplementary information: Supplementary data are available at Bioinformatics online. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

KSC-08pd1899

NASA Image and Video Library

2008-07-02

CAPE CANAVERAL, Fla. – NYIT MOCAP (Motion Capture) team Project Manager Jon Squitieri attaches a retro reflective marker to a motion capture suit worn by a technician who will be assembling the Orion Crew Module mockup. The motion tracking aims to improve efficiency of assembly processes and identify potential ergonomic risks for technicians assembling the mockup. The work is being performed in United Space Alliance's Human Engineering Modeling and Performance Lab in the RLV Hangar at NASA's Kennedy Space Center. Part of NASA's Constellation Program, the Orion spacecraft will return humans to the moon and prepare for future voyages to Mars and other destinations in our solar system.

Cavity Preparation/assembly Techniques and Impact on Q, Realistic Q - Factors in a Module, Review of Modules

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peter Kneisel

2005-03-19

This contribution summarizes the surface preparation procedures for niobium cavities presently used both in laboratory experiments and for modules, such as buffered chemical polishing (BCP), electropolishing (EP), high pressure ultrapure water rinsing (HPR), CO{sub 2} snow cleaning and high temperature heat treatments for hydrogen degassing or postpurification. The impact of surface treatments and the degree of cleanliness during assembly procedures on cavity performance (Q - value and accelerating gradient E{sub acc}) will be discussed. In addition, an attempt will be made to summarize the experiences made in module assemblies in different labs/projects such as DESY(TTF), Jlab (Upgrade) and SNS.

Magnetically suspended reaction wheel assembly

NASA Technical Reports Server (NTRS)

Stocking, G.

1984-01-01

The magnetically suspended reaction wheel assembly (MSRWA) is the product of a development effort funded by the Air Force Materials Laboratory (AFML) at Wright Patterson AFB. The specific objective of the project was to establish the manufacturing processes for samarium cobalt magnets and demonstrate their use in a space application. The development was successful on both counts. The application portion of the program, which involves the magnetically suspended reaction wheel assembly, is emphasized. The requirements for the reaction wheel were based on the bias wheel requirements of the DSP satellite. The tasks included the design, fabrication, and test of the unit to the DSP program qualification requirements.

Magnetically suspended reaction wheel assembly

NASA Astrophysics Data System (ADS)

Stocking, G.

1984-11-01

The magnetically suspended reaction wheel assembly (MSRWA) is the product of a development effort funded by the Air Force Materials Laboratory (AFML) at Wright Patterson AFB. The specific objective of the project was to establish the manufacturing processes for samarium cobalt magnets and demonstrate their use in a space application. The development was successful on both counts. The application portion of the program, which involves the magnetically suspended reaction wheel assembly, is emphasized. The requirements for the reaction wheel were based on the bias wheel requirements of the DSP satellite. The tasks included the design, fabrication, and test of the unit to the DSP program qualification requirements.

Constellation Program (CxP) Crew Exploration Vehicle (CEV) Parachute Assembly System (CPAS) Independent Design Reliability Assessment. Volume 1

NASA Technical Reports Server (NTRS)

Kelly, Michael J.

2010-01-01

This report documents the activities, findings, and NASA Engineering and Safety Center (NESC) recommendations of a multidiscipline team to independently assess the Constellation Program (CxP) Crew Exploration Vehicle (CEV) Parachute Assembly System (CPAS). This assessment occurred during a period of 15 noncontiguous months between December 2008 and April 2010, prior to the CPAS Project's Preliminary Design Review (PDR) in August 2010.

A Faster Fastener

NASA Technical Reports Server (NTRS)

2000-01-01

A licensing agreement between Marshall Space Flight Center and M&A Screw and Machineworks has brought the quick connect nut to the commerical market. Originally designed as part of a project seeking in-space assembly techniques, the quick connect nut is secured around a bolt merely by pushing it onto the bolt and giving it a single twist. Applications for the nuts include oil drilling platforms, mining industry, and other practices that rely on speedy assembly for success.

Cooling Acoustic Transducer with Heat Pipes

DTIC Science & Technology

2009-07-29

a heat sink. [0009] In Kan et al (United States Patent No. 6,528,909), a spindle motor assembly is disclosed which has a shaft with an integral...heat pipe. The shaft with the integral heat pipe improves the thermal conductively of the shaft and the spindle motor assembly. The shaft includes...Description of the Prior Art [0004] It is known in the art that transducers, designed to project acoustic power, are often limited by the build

75 FR 56093 - FFP Mass 1, LLC; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-15

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 13828-000] FFP Mass 1, LLC... study the feasibility of the Cape Cod Tidal Energy Project to be located in the Cape Cod Canal and a...,000 hydrokinetic tidal devices consisting of a turbine blade assembly and an integrated 10-kilowatt...

METHODS OF DETERMINING PUPIL READINESS FOR SPECIFIC UNITS OF INSTRUCTION PRESENTED THROUGH SIMULATED ENVIRONMENT MEDIA.

ERIC Educational Resources Information Center

PLUMPTON, RUSSEL A.

THE METHODS FOR DETERMINING PUPIL READINESS WERE STUDIED TO DEVELOP CRITERIA FOR PUPIL PARTICIPATION IN SIMULATED ENVIRONMENT LEARNING UNITS. THE LEARNING UNITS WERE SUBPROJECTS OF COOPERATIVE RESEARCH PROJECT NUMBER 1948 (ED 003 024). EACH OF THREE UNITS IN THE PROJECT WAS EXAMINED FOR PUPIL READINESS. TEST BATTERIES WERE ASSEMBLED AND…

The Reality of Welfare-to-Work: Employment Opportunities for Women Affected by Welfare Time Limits in Texas.

ERIC Educational Resources Information Center

Lawson, Leslie O.; King, Christopher T.

Researchers assembled a database of current and projected information on the following: welfare recipients; other female participants in the labor market; employment, occupational availability, and job openings; and occupational characteristics. The database was used in a multistep process to project the number of women forced to leave welfare…

PIR-1 and PIRPL. A Project in Robotics Education. Revised.

ERIC Educational Resources Information Center

Schultz, Charles P.

This paper presents the results of a project in robotics education that included: (1) designing a mobile robot--the Personal Instructional Robot-1 (PIR-1); (2) providing a guide to the purchase and assembly of necessary parts; (3) providing a way to interface the robot with common classroom microcomputers; and (4) providing a language by which the…

Genomics - the new rock and roll?

PubMed

Dunham, I

2000-10-01

The end of the beginning of the Human Genome Project was announced on 26 June when the working draft or first assembly was announced. Here, Ian Dunham who led the group at the Sanger Centre that produced the first complete sequence of a human chromosome reflects on how it felt to be with the genome project from the beginning.

Community Development, Popular Education, Financing of Adult Education. ASPBAE Courier No. 48.

ERIC Educational Resources Information Center

ASPBAE Courier, 1990

1990-01-01

Several of the articles in this document arose out of the International Council for Adult Education's World Assembly held in Bangkok, Thailand, in January 1990. The first article is on the 1989 evaluation of the Hilltribe Development Project, a long-term project undertaken in the north of Thailand. The article briefly describes various aspects of…

A Model for Deriving the Transfer Rate. Report of the Transfer Assembly Project.

ERIC Educational Resources Information Center

Jones, Enid B., Ed.

In 1989, a joint project was undertaken by the American Association of Community and Junior Colleges, the National Center for Academic Achievement and Transfer, the Center on Community College Education, and the Center for the Study of Community Colleges to disseminate data and research findings on the transfer of two-year college students to…

An Experimental Investigation of Leak Rate Performance of a Subscale Candidate Elastomer Docking Space Seal

NASA Technical Reports Server (NTRS)

Garafolo, Nicholas G.; Daniels, Christopher C.

2011-01-01

A novel docking seal was developed for the main interface seal of NASA s Low Impact Docking System (LIDS). This interface seal was designed to maintain acceptable leak rates while being exposed to the harsh environmental conditions of outer space. In this experimental evaluation, a candidate docking seal assembly called Engineering Development Unit (EDU58) was characterized and evaluated against the Constellation Project leak rate requirement. The EDU58 candidate seal assembly was manufactured from silicone elastomer S0383-70 vacuum molded in a metal retainer ring. Four seal designs were considered with unique characteristic heights. The leak rate performance was characterized through a mass point leak rate method by monitoring gas properties within an internal control volume. The leakage performance of the seals were described herein at representative docking temperatures of -50, +23, and +50 C for all four seal designs. Leak performance was also characterized at 100, 74, and 48 percent of full closure. For all conditions considered, the candidate seal assemblies met the Constellation Project leak rate requirement.

Kodak Mirror Assembly Tested at Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

2003-01-01

This photo (rear view) is of one of many segments of the Eastman-Kodak mirror assembly being tested for the James Webb Space Telescope (JWST) project at the X-Ray Calibration Facility at Marshall Space Flight Center (MSFC). MSFC is supporting Goddard Space Flight Center (GSFC) in developing the JWST by taking numerous measurements to predict its future performance. The tests are conducted in a vacuum chamber cooled to approximate the super cold temperatures found in space. During its 27 years of operation, the facility has performed testing in support of a wide array of projects, including the Hubble Space Telescope (HST), Solar A, Chandra technology development, Chandra High Resolution Mirror Assembly and science instruments, Constellation X-Ray Mission, and Solar X-Ray Imager, currently operating on a Geostationary Operational Environment Satellite. The JWST is NASA's next generation space telescope, a successor to the Hubble Space Telescope, named in honor of NASA's second administrator, James E. Webb. It is scheduled for launch in 2010 aboard an expendable launch vehicle. It will take about 3 months for the spacecraft to reach its destination, an orbit of 940,000 miles in space.

Adaptation of a Filter Assembly to Assess Microbial Bioburden of Pressurant Within a Propulsion System

NASA Technical Reports Server (NTRS)

Benardini, James N.; Koukol, Robert C.; Schubert, Wayne W.; Morales, Fabian; Klatte, Marlin F.

2012-01-01

A report describes an adaptation of a filter assembly to enable it to be used to filter out microorganisms from a propulsion system. The filter assembly has previously been used for particulates greater than 2 micrometers. Projects that utilize large volumes of nonmetallic materials of planetary protection concern pose a challenge to their bioburden budget, as a conservative specification value of 30 spores per cubic centimeter is typically used. Helium was collected utilizing an adapted filtration approach employing an existing Millipore filter assembly apparatus used by the propulsion team for particulate analysis. The filter holder on the assembly has a 47-mm diameter, and typically a 1.2-5 micrometer pore-size filter is used for particulate analysis making it compatible with commercially available sterilization filters (0.22 micrometers) that are necessary for biological sampling. This adaptation to an existing technology provides a proof-of-concept and a demonstration of successful use in a ground equipment system. This adaptation has demonstrated that the Millipore filter assembly can be utilized to filter out microorganisms from a propulsion system, whereas in previous uses the filter assembly was utilized for particulates greater than 2 micrometers.

Cascade Distiller System Performance Testing Interim Results

NASA Technical Reports Server (NTRS)

Callahan, Michael R.; Pensinger, Stuart; Sargusingh, Miriam J.

2014-01-01

The Cascade Distillation System (CDS) is a rotary distillation system with potential for greater reliability and lower energy costs than existing distillation systems. Based upon the results of the 2009 distillation comparison test (DCT) and recommendations of the expert panel, the Advanced Exploration Systems (AES) Water Recovery Project (WRP) project advanced the technology by increasing reliability of the system through redesign of bearing assemblies and improved rotor dynamics. In addition, the project improved the CDS power efficiency by optimizing the thermoelectric heat pump (TeHP) and heat exchanger design. Testing at the NASA-JSC Advanced Exploration System Water Laboratory (AES Water Lab) using a prototype Cascade Distillation Subsystem (CDS) wastewater processor (Honeywell d International, Torrance, Calif.) with test support equipment and control system developed by Johnson Space Center was performed to evaluate performance of the system with the upgrades as compared to previous system performance. The system was challenged with Solution 1 from the NASA Exploration Life Support (ELS) distillation comparison testing performed in 2009. Solution 1 consisted of a mixed stream containing human-generated urine and humidity condensate. A secondary objective of this testing is to evaluate the performance of the CDS as compared to the state of the art Distillation Assembly (DA) used in the ISS Urine Processor Assembly (UPA). This was done by challenging the system with ISS analog waste streams. This paper details the results of the AES WRP CDS performance testing.

CHP Integrated with Burners for Packaged Boilers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Castaldini, Carlo; Darby, Eric

2013-09-30

The objective of this project was to engineer, design, fabricate, and field demonstrate a Boiler Burner Energy System Technology (BBEST) that integrates a low-cost, clean burning, gas-fired simple-cycle (unrecuperated) 100 kWe (net) microturbine (SCMT) with a new ultra low-NOx gas-fired burner (ULNB) into one compact Combined Heat and Power (CHP) product that can be retrofit on new and existing industrial and commercial boilers in place of conventional burners. The Scope of Work for this project was segmented into two principal phases: (Phase I) Hardware development, assembly and pre-test and (Phase II) Field installation and demonstration testing. Phase I was dividedmore » into five technical tasks (Task 2 to 6). These tasks covered the engineering, design, fabrication, testing and optimization of each key component of the CHP system principally, ULNB, SCMT, assembly BBEST CHP package, and integrated controls. Phase I work culminated with the laboratory testing of the completed BBEST assembly prior to shipment for field installation and demonstration. Phase II consisted of two remaining technical tasks (Task 7 and 8), which focused on the installation, startup, and field verification tests at a pre-selected industrial plant to document performance and attainment of all project objectives. Technical direction and administration was under the management of CMCE, Inc. Altex Technologies Corporation lead the design, assembly and testing of the system. Field demonstration was supported by Leva Energy, the commercialization firm founded by executives at CMCE and Altex. Leva Energy has applied for patent protection on the BBEST process under the trade name of Power Burner and holds the license for the burner currently used in the product. The commercial term Power Burner is used throughout this report to refer to the BBEST technology proposed for this project. The project was co-funded by the California Energy Commission and the Southern California Gas Company (SCG), a division of Sempra Energy. These match funds were provided via concurrent contracts and investments available via CMCE, Altex, and Leva Energy The project attained all its objectives and is considered a success. CMCE secured the support of GI&E from Italy to supply 100 kW Turbec T-100 microturbines for the project. One was purchased by the project’s subcontractor, Altex, and a second spare was purchased by CMCE under this project. The microturbines were then modified to convert from their original recuperated design to a simple cycle configuration. Replacement low-NOx silo combustors were designed and bench tested in order to achieve compliance with the California Air Resources Board (CARB) 2007 emission limits for NOx and CO when in CHP operation. The converted microturbine was then mated with a low NOx burner provided by Altex via an integration section that allowed flow control and heat recovery to minimize combustion blower requirements; manage burner turndown; and recover waste heat. A new fully integrated control system was designed and developed that allowed one-touch system operation in all three available modes of operation: (1) CHP with both microturbine and burner firing for boiler heat input greater than 2 MMBtu/hr; (2) burner head only (BHO) when the microturbine is under service; and (3) microturbine only when boiler heat input requirements fall below 2 MMBtu/hr. This capability resulted in a burner turndown performance of nearly 10/1, a key advantage for this technology over conventional low NOx burners. Key components were then assembled into a cabinet with additional support systems for generator cooling and fuel supply. System checkout and performance tests were performed in the laboratory. The assembled system and its support equipment were then shipped and installed at a host facility where final performance tests were conducted following efforts to secure fabrication, air, and operating permits. The installed power burner is now in commercial operation and has achieved all the performance goals.« less

Origins of the Human Genome Project.

PubMed

Watson, J D; Cook-Deegan, R M

1991-01-01

The Human Genome Project has become a reality. Building on a debate that dates back to 1985, several genome projects are now in full stride around the world, and more are likely to form in the next several years. Italy began its genome program in 1987, and the United Kingdom and U.S.S.R. in 1988. The European communities mounted several genome projects on yeast, bacteria, Drosophila, and Arabidospis thaliana (a rapidly growing plant with a small genome) in 1988, and in 1990 commenced a new 2-year program on the human genome. In the United States, we have completed the first year of operation of the National Center for Human Genome Research at the National Institutes of Health (NIH), now the largest single funding source for genome research in the world. There have been dedicated budgets focused on genome-scale research at NIH, the U.S. Department of Energy, and the Howard Hughes Medical Institute for several years, and results are beginning to accumulate. There were three annual meetings on genome mapping and sequencing at Cold Spring Harbor, New York, in the spring of 1988, 1989, and 1990; the talks have shifted from a discussion about how to approach problems to presenting results from experiments already performed. We have finally begun to work rather than merely talk. The purpose of genome projects is to assemble data on the structure of DNA in human chromosomes and those of other organisms. A second goal is to develop new technologies to perform mapping and sequencing. There have been impressive technical advances in the past 5 years since the debate about the human genome project began. We are on the verge of beginning pilot projects to test several approaches to sequencing long stretches of DNA, using both automation and manual methods. Ordered sets of yeast artificial chromosome and cosmid clones have been assembled to span more than 2 million base pairs of several human chromosomes, and a region of 10 million base pairs has been assembled for Caenorhabditis elegans by a collaboration between Washington University and the Medical Research Council laboratory in Cambridge, U.K. This project is now turning to sequencing C. elegans DNA as a logical extension of this work. These are but the first fruits of the genome project. There is much more to come.

Development of NASA's Sample Cartridge Assembly: Design, Thermal Analysis, and Testing

NASA Technical Reports Server (NTRS)

O'Connor, Brian; Hernandez, Deborah; Duffy, James

2015-01-01

NASA's Sample Cartridge Assembly (SCA) project is responsible for designing and validating a payload that contains a materials research sample in a sealed environment. The SCA will be heated in the European Space Agency's (ESA) Low Gradient Furnace (LGF) that is housed inside the Material Science Research Rack (MSRR) located in the International Space Station (ISS). Sintered metals and crystal growth experiments in microgravity are examples of some of the types of materials research that may be performed with a SCA. The project's approach has been to use thermal models to guide the SCA through several design iterations. Various layouts of the SCA components were explored to meet the science and engineering requirements, and testing has been done to help prove the design. This paper will give an overview of the SCA design. It will show how thermal analysis is used to support the project. Also some testing that has been completed will also be discussed, including changes that were made to the thermal profile used during brazing.

Reliability and Qualification of Hardware to Enhance the Mission Assurance of JPL/NASA Projects

NASA Technical Reports Server (NTRS)

Ramesham, Rajeshuni

2010-01-01

Packaging Qualification and Verification (PQV) and life testing of advanced electronic packaging, mechanical assemblies (motors/actuators), and interconnect technologies (flip-chip), platinum temperature thermometer attachment processes, and various other types of hardware for Mars Exploration Rover (MER)/Mars Science Laboratory (MSL), and JUNO flight projects was performed to enhance the mission assurance. The qualification of hardware under extreme cold to hot temperatures was performed with reference to various project requirements. The flight like packages, assemblies, test coupons, and subassemblies were selected for the study to survive three times the total number of expected temperature cycles resulting from all environmental and operational exposures occurring over the life of the flight hardware including all relevant manufacturing, ground operations, and mission phases. Qualification/life testing was performed by subjecting flight-like qualification hardware to the environmental temperature extremes and assessing any structural failures, mechanical failures or degradation in electrical performance due to either overstress or thermal cycle fatigue. Experimental flight qualification test results will be described in this presentation.

A preliminary evaluation of the ability of from-reactor casks to geometrically accommodate commercial LWR spent nuclear fuel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Andress, D.; Joy, D.S.; McLeod, N.B.

The Department of Energy has sponsored a number of cask design efforts to define several transportation casks to accommodate the various assemblies expected to be accepted by the Federal Waste Management System. At this time, three preliminary cask designs have been selected for the final design--the GA-4 and GA-9 truck casks and the BR-100 rail cask. In total, this assessment indicates that the current Initiative I cask designs can be expected to dimensionally accommodate 100% of the PWR fuel assemblies (other than the extra-long South Texas Fuel) with control elements removed, and >90% of the assemblies having the control elementsmore » as an integral part of the fuel assembly. For BWR assemblies, >99% of the assemblies can be accommodated with fuel channels removed. This paper summarizes preliminary results of one part of that evaluation related to the ability of the From-Reactor Initiative I casks to accommodate the physical and radiological characteristics of the Spent Nuclear Fuel projected to be accepted into the Federal Waste Management System. 3 refs., 5 tabs.« less

Seven ways to make a hypertext project fail

NASA Technical Reports Server (NTRS)

Glushko, Robert J.

1990-01-01

Hypertext is an exciting concept, but designing and developing hypertext applications of practical scale is hard. To make a project feasible and successful 'hypertext engineers' must overcome the following problems: (1) developing realistic expectations in the face of hypertext hype; (2) assembling a multidisciplinary project team; (3) establishing and following design guidelines; (4) dealing with installed base constraints; (5) obtaining usable source files; (6) finding appropriate software technology and methods; and (7) overcoming legal uncertainties about intellectual property concerns.

Support for Astronaut's View of Mexican/ Central American Fires and on-Line Earth Observations Training Manual

NASA Technical Reports Server (NTRS)

Kaminski, Charles F., Jr.

1999-01-01

A small project to compile remote sensing and in-site data to review the processes leading to the May 1998 Mexican/Central American fires was undertaken. A web page based on this project was assembled. The second project initiated involved an interactive and on-line program that will replace the paper version of the Earth Observations Preflight Training Manual. Technical support was provided to Prof. Marvin Glasser as needed.

Fabrication of the HIAD Large-Scale Demonstration Assembly and Upcoming Mission Applications

NASA Technical Reports Server (NTRS)

Swanson, G. T.; Johnson, R. K.; Hughes, S. J.; Dinonno, J. M.; Cheatwood, F M.

2017-01-01

Over a decade of work has been conducted in the development of NASAs Hypersonic Inflatable Aerodynamic Decelerator (HIAD) technology. This effort has included multiple ground test campaigns and flight tests culminating in the HIAD projects second generation (Gen-2) deployable aeroshell system and associated analytical tools. NASAs HIAD project team has developed, fabricated, and tested inflatable structures (IS) integrated with flexible thermal protection system (F-TPS), ranging in diameters from 3-6m, with cone angles of 60 and 70 deg.In 2015, United Launch Alliance (ULA) announced that they will use a HIAD (10-12m) as part of their Sensible, Modular, Autonomous Return Technology (SMART) for their upcoming Vulcan rocket. ULA expects SMART reusability, coupled with other advancements for Vulcan, will substantially reduce the cost of access to space. The first booster engine recovery via HIAD is scheduled for 2024. To meet this near-term need, as well as future NASA applications, the HIAD team is investigating taking the technology to the 10-15m diameter scale.In the last year, many significant development and fabrication efforts have been accomplished, culminating in the construction of a large-scale inflatable structure demonstration assembly. This assembly incorporated the first three tori for a 12m Mars Human-Scale Pathfinder HIAD conceptual design that was constructed with the current state of the art material set. Numerous design trades and torus fabrication demonstrations preceded this effort. In 2016, three large-scale tori (0.61m cross-section) and six subscale tori (0.25m cross-section) were manufactured to demonstrate fabrication techniques using the newest candidate material sets. These tori were tested to evaluate durability and load capacity. This work led to the selection of the inflatable structures third generation (Gen-3) structural liner. In late 2016, the three tori required for the large-scale demonstration assembly were fabricated, and then integrated in early 2017. The design includes provisions to add the remaining four tori necessary to complete the assembly of the 12m Human-Scale Pathfinder HIAD in the event future project funding becomes available.This presentation will discuss the HIAD large-scale demonstration assembly design and fabrication per-formed in the last year including the precursor tori development and the partial-stack fabrication. Potential near-term and future 10-15m HIAD applications will also be discussed.

Fabrication of the HIAD Large-Scale Demonstration Assembly

NASA Technical Reports Server (NTRS)

Swanson, G. T.; Johnson, R. K.; Hughes, S. J.; DiNonno, J. M.; Cheatwood, F. M.

2017-01-01

Over a decade of work has been conducted in the development of NASA's Hypersonic Inflatable Aerodynamic Decelerator (HIAD) technology. This effort has included multiple ground test campaigns and flight tests culminating in the HIAD projects second generation (Gen-2) deployable aeroshell system and associated analytical tools. NASAs HIAD project team has developed, fabricated, and tested inflatable structures (IS) integrated with flexible thermal protection system (F-TPS), ranging in diameters from 3-6m, with cone angles of 60 and 70 deg.In 2015, United Launch Alliance (ULA) announced that they will use a HIAD (10-12m) as part of their Sensible, Modular, Autonomous Return Technology (SMART) for their upcoming Vulcan rocket. ULA expects SMART reusability, coupled with other advancements for Vulcan, will substantially reduce the cost of access to space. The first booster engine recovery via HIAD is scheduled for 2024. To meet this near-term need, as well as future NASA applications, the HIAD team is investigating taking the technology to the 10-15m diameter scale. In the last year, many significant development and fabrication efforts have been accomplished, culminating in the construction of a large-scale inflatable structure demonstration assembly. This assembly incorporated the first three tori for a 12m Mars Human-Scale Pathfinder HIAD conceptual design that was constructed with the current state of the art material set. Numerous design trades and torus fabrication demonstrations preceded this effort. In 2016, three large-scale tori (0.61m cross-section) and six subscale tori (0.25m cross-section) were manufactured to demonstrate fabrication techniques using the newest candidate material sets. These tori were tested to evaluate durability and load capacity. This work led to the selection of the inflatable structures third generation (Gen-3) structural liner. In late 2016, the three tori required for the large-scale demonstration assembly were fabricated, and then integrated in early 2017. The design includes provisions to add the remaining four tori necessary to complete the assembly of the 12m Human-Scale Pathfinder HIAD in the event future project funding becomes available.This presentation will discuss the HIAD large-scale demonstration assembly design and fabrication per-formed in the last year including the precursor tori development and the partial-stack fabrication. Potential near-term and future 10-15m HIAD applications will also be discussed.

Mini-Brayton heat source assembly design study. Volume 2: Titan 3C mission. [minimum weight modifications

NASA Technical Reports Server (NTRS)

1973-01-01

Major conclusions of the space shuttle heat source assembly study are reported that project a minimum weight design for a Titan 3 C synchronous orbit mission; requirements to recover the heat source in orbit are eliminated. This concept permits location of the heat source end enclosure supports and heat source assembly support housing in a low temperature region external to the insulation enclosure and considers titanium and beryllium alloys for these support elements. A high melting insulation blanket consisting of nickel foil coated with zirconia, or of gold foil separated with glass fiber layers, is selected to provide emergency cooling in the range 2000 to 2700 F to prevent the isotope heat source from reaching unsafe temperatures. A graphic view of the baseline heat source assembly is included.

Ten steps to get started in Genome Assembly and Annotation

PubMed Central

Dominguez Del Angel, Victoria; Hjerde, Erik; Sterck, Lieven; Capella-Gutierrez, Salvadors; Notredame, Cederic; Vinnere Pettersson, Olga; Amselem, Joelle; Bouri, Laurent; Bocs, Stephanie; Klopp, Christophe; Gibrat, Jean-Francois; Vlasova, Anna; Leskosek, Brane L.; Soler, Lucile; Binzer-Panchal, Mahesh; Lantz, Henrik

2018-01-01

As a part of the ELIXIR-EXCELERATE efforts in capacity building, we present here 10 steps to facilitate researchers getting started in genome assembly and genome annotation. The guidelines given are broadly applicable, intended to be stable over time, and cover all aspects from start to finish of a general assembly and annotation project. Intrinsic properties of genomes are discussed, as is the importance of using high quality DNA. Different sequencing technologies and generally applicable workflows for genome assembly are also detailed. We cover structural and functional annotation and encourage readers to also annotate transposable elements, something that is often omitted from annotation workflows. The importance of data management is stressed, and we give advice on where to submit data and how to make your results Findable, Accessible, Interoperable, and Reusable (FAIR). PMID:29568489

Crystallization-driven assembly of conjugated-polymer-based nanostructures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hayward, Ryan C.

2016-10-15

The goal of this project has been to improve our ability to simultaneously control the organization, and therefore the opto-electronic properties, of conjugated-polymer based materials across three different length-scales: 1) the molecular scale, in the sense of controlling growth and functionalization of highly crystalline semiconducting organic materials capable of efficient charge transport, 2) the nanoscale, in terms of positioning n- and p-type materials with domain sizes comparable to exciton diffusion lengths (~ 10 nm) to facilitate charge separation, and 3) the colloidal scale, such that well-defined crystalline nanoscale building blocks can be hierarchically organized into device layers. As described inmore » more detail below, the project was successful in generating powerful new approaches to, and improved fundamental understanding of, processing and self-assembly of organic and hybrid semiconducting materials across all three length-scales. Although the goals of the project were formulated with primarily photovoltaic architectures in mind, the outcomes of the project have significant implications for a variety of conjugated-polymer-based devices including field-effect-transistors for sensors and logic devices, as well as potentially thermoelectrics and battery electrode materials. The project has resulted in 10 peer-reviewed publications to date [1-10], with several additional manuscripts currently in preparation.« less

Project W-320, 241-C-106 sluicing electrical calculations, Volume 2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bailey, J.W.

1998-08-07

This supporting document has been prepared to make the FDNW calculations for Project W-320, readily retrievable. These calculations are required: To determine the power requirements needed to power electrical heat tracing segments contained within three manufactured insulated tubing assemblies; To verify thermal adequacy of tubing assembly selection by others; To size the heat tracing feeder and branch circuit conductors and conduits; To size protective circuit breaker and fuses; and To accomplish thermal design for two electrical heat tracing segments: One at C-106 tank riser 7 (CCTV) and one at the exhaust hatchway (condensate drain). Contents include: C-Farm electrical heat tracing;more » Cable ampacity, lighting, conduit fill and voltage drop; and Control circuit sizing and voltage drop analysis for the seismic shutdown system.« less

Improving Robotic Assembly of Planar High Energy Density Targets

NASA Astrophysics Data System (ADS)

Dudt, D.; Carlson, L.; Alexander, N.; Boehm, K.

2016-10-01

Increased quantities of planar assemblies for high energy density targets are needed with higher shot rates being implemented at facilities such as the National Ignition Facility and the Matter in Extreme Conditions station of the Linac Coherent Light Source. To meet this growing demand, robotics are used to reduce assembly time. This project studies how machine vision and force feedback systems can be used to improve the quantity and quality of planar target assemblies. Vision-guided robotics can identify and locate parts, reducing laborious manual loading of parts into precision pallets and associated teaching of locations. On-board automated inspection can measure part pickup offsets to correct part drop-off placement into target assemblies. Force feedback systems can detect pickup locations and apply consistent force to produce more uniform glue bond thickness, thus improving the performance of the targets. System designs and performance evaluations will be presented. Work supported in part by the US DOE under the Science Undergraduate Laboratory Internships Program (SULI) and ICF Target Fabrication DE-NA0001808.

Finishing bacterial genome assemblies with Mix.

PubMed

Soueidan, Hayssam; Maurier, Florence; Groppi, Alexis; Sirand-Pugnet, Pascal; Tardy, Florence; Citti, Christine; Dupuy, Virginie; Nikolski, Macha

2013-01-01

Among challenges that hamper reaping the benefits of genome assembly are both unfinished assemblies and the ensuing experimental costs. First, numerous software solutions for genome de novo assembly are available, each having its advantages and drawbacks, without clear guidelines as to how to choose among them. Second, these solutions produce draft assemblies that often require a resource intensive finishing phase. In this paper we address these two aspects by developing Mix , a tool that mixes two or more draft assemblies, without relying on a reference genome and having the goal to reduce contig fragmentation and thus speed-up genome finishing. The proposed algorithm builds an extension graph where vertices represent extremities of contigs and edges represent existing alignments between these extremities. These alignment edges are used for contig extension. The resulting output assembly corresponds to a set of paths in the extension graph that maximizes the cumulative contig length. We evaluate the performance of Mix on bacterial NGS data from the GAGE-B study and apply it to newly sequenced Mycoplasma genomes. Resulting final assemblies demonstrate a significant improvement in the overall assembly quality. In particular, Mix is consistent by providing better overall quality results even when the choice is guided solely by standard assembly statistics, as is the case for de novo projects. Mix is implemented in Python and is available at https://github.com/cbib/MIX, novel data for our Mycoplasma study is available at http://services.cbib.u-bordeaux2.fr/mix/.

Treatment planning capability assessment of a beam shaping assembly for accelerator-based BNCT.

PubMed

Herrera, M S; González, S J; Burlon, A A; Minsky, D M; Kreiner, A J

2011-12-01

Within the frame of an ongoing project to develop a folded Tandem-Electrostatic-Quadrupole accelerator facility for Accelerator-Based Boron Neutron Capture Therapy (AB-BNCT) a theoretical study was performed to assess the treatment planning capability of different configurations of an optimized beam shaping assembly for such a facility. In particular this study aims at evaluating treatment plans for a clinical case of Glioblastoma. Copyright © 2011 Elsevier Ltd. All rights reserved.

Moisture and Structural Analysis for High Performance Hybrid Wall Assemblies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grin, A.; Lstiburek, J.

2012-09-01

Based on past experience in the Building America program, BSC has found that combinations of materials and approaches—in other words, systems—usually provide optimum performance. Integration is necessary, as described in this research project. The hybrid walls analyzed utilize a combination of exterior insulation, diagonal metal strapping, and spray polyurethane foam and leave room for cavity-fill insulation. These systems can provide effective thermal, air, moisture, and water barrier systems in one assembly and provide structure.

Moisture and Structural Analysis for High Performance Hybrid Wall Assemblies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grin, A.; Lstiburek, J.

2012-09-01

This report describes the work conducted by the Building Science Corporation (BSC) Building America Research Team's 'Energy Efficient Housing Research Partnerships' project. Based on past experience in the Building America program, they have found that combinations of materials and approaches---in other words, systems--usually provide optimum performance. No single manufacturer typically provides all of the components for an assembly, nor has the specific understanding of all the individual components necessary for optimum performance.

Constellation Program (CxP) Crew Exploration Vehicle (CEV) Parachute Assembly System (CPAS) Independent Design Reliability Assessment. Volume 2; Appendices

NASA Technical Reports Server (NTRS)

Kelly, Michael J.

2010-01-01

This document contains the Appendices to the report documenting the activities, findings, and NASA Engineering and Safety Center (NESC) recommendations of a multidiscipline team to independently assess the Constellation Program (CxP) Crew Exploration Vehicle (CEV) Parachute Assembly System (CPAS). The assessment occurred during a period of 15 noncontiguous months between December 2008 and April 2010, prior to the CPAS Project's Preliminary Design Review (PDR) in August 2010.

MELCOR model for an experimental 17x17 spent fuel PWR assembly.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cardoni, Jeffrey

2010-11-01

A MELCOR model has been developed to simulate a pressurized water reactor (PWR) 17 x 17 assembly in a spent fuel pool rack cell undergoing severe accident conditions. To the extent possible, the MELCOR model reflects the actual geometry, materials, and masses present in the experimental arrangement for the Sandia Fuel Project (SFP). The report presents an overview of the SFP experimental arrangement, the MELCOR model specifications, demonstration calculation results, and the input model listing.

Cooling Acoustic Transcucer with Heat Pipes

DTIC Science & Technology

2009-07-19

circuits to a heat sink. [0009] In Kan et al (United States Patent No. 6,528,909), a spindle motor assembly is disclosed which has a shaft with an...integral heat pipe. The shaft with the integral heat pipe improves the thermal conductively of the shaft and the spindle motor assembly. The shaft ...2) Description of the Prior Art [0004] It is known in the art that transducers, designed to project acoustic power, are often limited by the

Evaluation of the influence of dominance rules for the assembly line design problem under consideration of product design alternatives

NASA Astrophysics Data System (ADS)

Oesterle, Jonathan; Lionel, Amodeo

2018-06-01

The current competitive situation increases the importance of realistically estimating product costs during the early phases of product and assembly line planning projects. In this article, several multi-objective algorithms using difference dominance rules are proposed to solve the problem associated with the selection of the most effective combination of product and assembly lines. The list of developed algorithms includes variants of ant colony algorithms, evolutionary algorithms and imperialist competitive algorithms. The performance of each algorithm and dominance rule is analysed by five multi-objective quality indicators and fifty problem instances. The algorithms and dominance rules are ranked using a non-parametric statistical test.

Multiple pure tone elimination strut assembly. [air breathing engines

NASA Technical Reports Server (NTRS)

Burcham, F. W. (Inventor)

1981-01-01

An acoustic noise elimination assembly is disclosed which has a capability for disrupting the continuity of fields of sound pressures forwardly projected from fans or rotors of a type commonly found in the fan or compressor first stage for air-breathing engines, when operating at tip speeds in the supersonic range. The assembly includes a tubular cowl defining a duct for delivering an air stream axially into the intake for a jet engine. A sound barrier, defined by a number of intersecting flat plates or struts has a line of intersection coincident with a longitudinal axis of the tubular cowl, which serves to disrupt the continuity of rotating fields of multiple pure tonal components of noise.

(n,xn) cross section measurements for Y-89 foils used as detectors for high energy neutron measurements in the deeply subcritical assembly "QUINTA"

NASA Astrophysics Data System (ADS)

Bielewicz, Marcin; Kilim, Stanisław; Strugalska-Gola, Elżbieta; Szuta, Marcin; Wojciechowski, Andrzej; Tyutyunnikov, Sergey; Prokofiev, Alexander; Passoth, Elke

2017-09-01

Study of the deep subcritical systems (QUINTA) using relativistic beams is performed within the project "Energy and Transmutation of Radioactive Wastes" (E&T - RAW). The experiment assembly was irradiated by deuteron/proton beam (Dubna NUCLOTRON). We calculated the neutron energy spectrum inside the whole assembly by using threshold energy (n,xn) reactions in yttrium (Y-89) foils. There are almost no experimental cross section data for those reactions. New Y-89(n,xn) cross section measurements were carried out at The Svedberg laboratory (TSL) in Uppsala, Sweden in 2015. In this paper we present preliminary results of those experiments.

Mars Science Laboratory Spacecraft Assembled for Testing

NASA Technical Reports Server (NTRS)

2008-01-01

The major components of NASA's Mars Science Laboratory spacecraft cruise stage atop the aeroshell, which has the descent stage and rover inside were connected together in October 2008 for several weeks of system testing, including simulation of launch vibrations and deep-space environmental conditions.

These components will be taken apart again, for further work on each of them, after the environmental testing. The Mars Science Laboratory spacecraft is being assembled and tested for launch in 2011.

This image was taken inside the Spacecraft Assembly Facility at NASA's Jet Propulsion Laboratory, Pasadena, Calif., which manages the Mars Science Laboratory Project for the NASA Science Mission Directorate, Washington. JPL is a division of the California Institute of Technology.

Report of Library Services and Construction Act Project # 2842, July 1 - December 31, 1966.

ERIC Educational Resources Information Center

Los Angeles Public Library, CA.

This report covers the second six months of the Los Angeles Public Library's project to extend library service to the disadvantaged. In the second period emphasis was placed on involvement with individuals as well as with community groups, on development of techniques, and on assembling a variety of materials for reaching and working with…

Production Line Materials. Youth Training Scheme. Core Exemplar Work Based Project.

ERIC Educational Resources Information Center

Further Education Staff Coll., Blagdon (England).

This trainer's guide is intended to assist supervisors of work-based career training projects in helping students understand the operation of an assembly line, including safe working procedures. The guide is one in a series of core curriculum modules that is intended for use in combination on- and off-the-job programs to familiarize youth with the…

Energetic and Environmental Impacts Related to Transport and Assembling Processes in ABiogas Production Plant from Marine Macroalgae (FP7 Project BioWALK4Biofuels)

NASA Astrophysics Data System (ADS)

Cappelli, Andrea; Gigli, Emanuele; Muzi, Luca; Renda, Roberto; Simoni, Silvano

2010-01-01

GHG emissions, eutrophication and energy dependence are problems that the EU has to face in the near future. The BioWALK4Biofuels project aims to find a common response to these challenges, taking advantage of spontaneous biological processes: the growth of algae and anaerobic digestion of biomass. This project is being built thanks to European funding under the 7th Framework Programme. To evaluate the results obtained, a first LCA study was carried out that, as regards the data on infrastructure and on the assembly of the plant, refers to data supplied by manufacturers, while the study of algal growth was made on the basis of a model of cultivation that takes account of the aspects that most affect this key process among all the ones that cooperate in the whole plant. The electricity and heat produced through a co-generator fueled by biogas produced from algal biomass, according to this study, are responsible for GHG emissions reduced by 52% compared to traditional technologies. The biogas produced during the 4 years of the project allows the substitution of 85 tonnes of oil equivalent (toe).

GI-POP: a combinational annotation and genomic island prediction pipeline for ongoing microbial genome projects.

PubMed

Lee, Chi-Ching; Chen, Yi-Ping Phoebe; Yao, Tzu-Jung; Ma, Cheng-Yu; Lo, Wei-Cheng; Lyu, Ping-Chiang; Tang, Chuan Yi

2013-04-10

Sequencing of microbial genomes is important because of microbial-carrying antibiotic and pathogenetic activities. However, even with the help of new assembling software, finishing a whole genome is a time-consuming task. In most bacteria, pathogenetic or antibiotic genes are carried in genomic islands. Therefore, a quick genomic island (GI) prediction method is useful for ongoing sequencing genomes. In this work, we built a Web server called GI-POP (http://gipop.life.nthu.edu.tw) which integrates a sequence assembling tool, a functional annotation pipeline, and a high-performance GI predicting module, in a support vector machine (SVM)-based method called genomic island genomic profile scanning (GI-GPS). The draft genomes of the ongoing genome projects in contigs or scaffolds can be submitted to our Web server, and it provides the functional annotation and highly probable GI-predicting results. GI-POP is a comprehensive annotation Web server designed for ongoing genome project analysis. Researchers can perform annotation and obtain pre-analytic information include possible GIs, coding/non-coding sequences and functional analysis from their draft genomes. This pre-analytic system can provide useful information for finishing a genome sequencing project. Copyright © 2012 Elsevier B.V. All rights reserved.

X-ray satellite

NASA Technical Reports Server (NTRS)

1985-01-01

An overview of the second quarter 1985 development of the X-ray satellite project is presented. It is shown that the project is proceeding according to plan and that the projected launch date of September 9, 1987 is on schedule. An overview of the work completed and underway on the systems, subsystems, payload, assembly, ground equipment and interfaces is presented. Problem areas shown include cost increases in the area of focal instrumentation, the star sensor light scattering requirements, and postponements in the data transmission subsystems.

Turn Basin Construction

NASA Image and Video Library

2017-06-30

Across from the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, cement trucks stand by to support a construction project to upgrade the turn basin wharf. The work includes driving multiple precast concrete piles to a depth of about 70 feet to accommodate arrival of the core stage for the agency's Space Launch System (SLS) rocket. When the stage for NASA's SLS departs the Michoud Assembly Facility in New Orleans, it will be shipped by the agency's modified barge to the Launch Complex 39 turn basin.

Turn Basin Construction

NASA Image and Video Library

2017-06-30

Across from the Vehicle Assembly Building at NASA's Kennedy Space Center in Florida, cement is poured as part of a construction project to upgrade the turn basin wharf. The work includes driving multiple precast concrete piles to a depth of about 70 feet to accommodate arrival of the core stage for the agency's Space Launch System (SLS) rocket. When the stage for NASA's SLS departs the Michoud Assembly Facility in New Orleans, it will be shipped by the agency's modified barge to the Launch Complex 39 turn basin.

A 1-D Model of the 4 Bed Molecular Sieve of the Carbon Dioxide Removal Assembly

NASA Technical Reports Server (NTRS)

Coker, Robert; Knox, Jim

2015-01-01

Developments to improve system efficiency and reliability for water and carbon dioxide separation systems on crewed vehicles combine sub-scale systems testing and multi-physics simulations. This paper describes the development of COMSOL simulations in support of the Life Support Systems (LSS) project within NASA's Advanced Exploration Systems (AES) program. Specifically, we model the 4 Bed Molecular Sieve (4BMS) of the Carbon Dioxide Removal Assembly (CDRA) operating on the International Space Station (ISS).

Mars aerobrake assembly simulation

NASA Technical Reports Server (NTRS)

Filatovs, G. J.; Lee, Gordon K. F.; Garvey, John

1992-01-01

On-orbit assembly operation simulations in neutral buoyancy conditions are presently undertaken by a partial/full-scale Mars mission aerobrake mockup, whose design, conducted in the framework of an engineering senior students' design project, involved several levels of constraints for critical physical and operational features. Allowances had to be made for the auxiliary constraints introduced by underwater testing, as well as the subsegmenting required for overland shipment to the neutral-buoyancy testing facility. This mockup aerobrake's fidelity is determined by the numerous, competing design objectives.

FLOAT OPERATED RADIAL GATE HOIST ASSEMBLY LIST OF PARTS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

FLOAT OPERATED RADIAL GATE HOIST ASSEMBLY - LIST OF PARTS - BASE-CRANK. WASTEWAY NO. 1. WELLTON-MOHAWK CANAL - STA. 99+23.50. United States Department of the Interior, Bureau of Reclamation; Gila Project, Arizona, Wellton-Mohawk Division. Drawing No. 50-D-2511, dated May 3, 1949, Denver Colorado. Sheet 1 of 2 - Wellton-Mohawk Irrigation System, Wasteway No. 1, Wellton-Mohawk Canal, North side of Wellton-Mohawk Canal, bounded by Gila River to North & the Union Pacific Railroad & Gila Mountains to south, Wellton, Yuma County, AZ

Components, Assembly and Electrochemical Properties of Three-Dimensional Battery Architectures

DTIC Science & Technology

2016-03-01

batteries is directed at our project on 3-D lithium - ion batteries where improvements in materials and fabrication methods are expected to facilitate...reporting period, we focused on new materials and electrode array fabrication processes for 3-D lithium - ion batteries and made substantial progress. In...to facilitate the assembly of a full 3-D lithium - ion battery system. a Pattern silicon dioxide etch I I I I I mask b DRIE etch silicon posts c I I

BAC sequencing using pooled methods.

PubMed

Saski, Christopher A; Feltus, F Alex; Parida, Laxmi; Haiminen, Niina

2015-01-01

Shotgun sequencing and assembly of a large, complex genome can be both expensive and challenging to accurately reconstruct the true genome sequence. Repetitive DNA arrays, paralogous sequences, polyploidy, and heterozygosity are main factors that plague de novo genome sequencing projects that typically result in highly fragmented assemblies and are difficult to extract biological meaning. Targeted, sub-genomic sequencing offers complexity reduction by removing distal segments of the genome and a systematic mechanism for exploring prioritized genomic content through BAC sequencing. If one isolates and sequences the genome fraction that encodes the relevant biological information, then it is possible to reduce overall sequencing costs and efforts that target a genomic segment. This chapter describes the sub-genome assembly protocol for an organism based upon a BAC tiling path derived from a genome-scale physical map or from fine mapping using BACs to target sub-genomic regions. Methods that are described include BAC isolation and mapping, DNA sequencing, and sequence assembly.

Genome Sequencing and Assembly by Long Reads in Plants

PubMed Central

Li, Changsheng; Lin, Feng; An, Dong; Huang, Ruidong

2017-01-01

Plant genomes generated by Sanger and Next Generation Sequencing (NGS) have provided insight into species diversity and evolution. However, Sanger sequencing is limited in its applications due to high cost, labor intensity, and low throughput, while NGS reads are too short to resolve abundant repeats and polyploidy, leading to incomplete or ambiguous assemblies. The advent and improvement of long-read sequencing by Third Generation Sequencing (TGS) methods such as PacBio and Nanopore have shown promise in producing high-quality assemblies for complex genomes. Here, we review the development of sequencing, introducing the application as well as considerations of experimental design in TGS of plant genomes. We also introduce recent revolutionary scaffolding technologies including BioNano, Hi-C, and 10× Genomics. We expect that the informative guidance for genome sequencing and assembly by long reads will benefit the initiation of scientists’ projects. PMID:29283420

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dudek, L.; Chrzanowski, J.; Heitzenroeder, P.

The National Compact Stellarator Experiment (NCSX) has been under construction at the Princeton Plasma Physics Laboratory (PPPL) in partnership with the Oak Ridge National Laboratory (ORNL). The stellarator core is designed to produce a compact 3D plasma that combines stellarator and tokamak physics advantages. The complex geometry and tight fabrication tolerances of NCSX create some unique engineering and assembly challenges. The NCSX project was cancelled in May 2008; construction activities are presently being phased out in an orderly fashion. This paper will describe the progress of the fabrication and assembly activities of NCSX. Completion of the coil fabrication is onmore » track for the summer of 2008. All three of the vacuum vessel 120 degrees sections have been delivered. Assembly of vacuum vessel services began in May 2006 and is now complete. Assembly of the modular coils into 3-packs for safe storage is presently underway. (C) 2008 Elsevier B.V. All rights reserved.« less

Multiparameter vision testing apparatus

NASA Technical Reports Server (NTRS)

Hunt, S. R., Jr.; Homkes, R. J.; Poteate, W. B.; Sturgis, A. C. (Inventor)

1975-01-01

Compact vision testing apparatus is described for testing a large number of physiological characteristics of the eyes and visual system of a human subject. The head of the subject is inserted into a viewing port at one end of a light-tight housing containing various optical assemblies. Visual acuity and other refractive characteristics and ocular muscle balance characteristics of the eyes of the subject are tested by means of a retractable phoroptor assembly carried near the viewing port and a film cassette unit carried in the rearward portion of the housing (the latter selectively providing a variety of different visual targets which are viewed through the optical system of the phoroptor assembly). The visual dark adaptation characteristics and absolute brightness threshold of the subject are tested by means of a projector assembly which selectively projects one or both of a variable intensity fixation target and a variable intensity adaptation test field onto a viewing screen located near the top of the housing.

Viper cabin-fuselage structural design concept with engine installation and wing structural design

NASA Technical Reports Server (NTRS)

Marchesseault, B.; Carr, D.; Mccorkle, T.; Stevens, C.; Turner, D.

1993-01-01

This report describes the process and considerations in designing the cabin, nose, drive shaft, and wing assemblies for the 'Viper' concept aircraft. Interfaces of these assemblies, as well as interfaces with the sections of the aircraft aft of the cabin, are also discussed. The results of the design process are included. The goal of this project is to provide a structural design which complies with FAR 23 requirements regarding occupant safety, emergency landing loads, and maneuvering loads. The design must also address the interfaces of the various systems in the cabin, nose, and wing, including the drive shaft, venting, vacuum, electrical, fuel, and control systems. Interfaces between the cabin assembly and the wing carrythrough and empennage assemblies were required, as well. In the design of the wing assemblies, consistency with the existing cabin design was required. The major areas considered in this report are materials and construction, loading, maintenance, environmental considerations, wing assembly fatigue, and weight. The first three areas are developed separately for the nose, cabin, drive shaft, and wing assemblies, while the last three are discussed for the entire design. For each assembly, loading calculations were performed to determine the proper sizing of major load carrying components. Table 1.0 lists the resulting margins of safety for these key components, along with the types of the loads involved, and the page number upon which they are discussed.

80. photographer unknown 7 June 1937 PLACING MAIN THRUST BEARING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

80. photographer unknown 7 June 1937 PLACING MAIN THRUST BEARING ASSEMBLY IN UNIT NO 2. - Bonneville Project, Powerhouse No.1, Spanning Bradford Slough, from Bradford Island, Bonneville, Multnomah County, OR

OAS :: Accountability :: Program and Project Evaluation Reports

Science.gov Websites

Disarmament Drugs E e-Government Education Elections Environment Equity G General Assembly Governance H Human Management Public Security R Racism and Intolerance Refugees S Scholarships School of Governance Science and

Large Synoptic Survey Telescope mount final design

NASA Astrophysics Data System (ADS)

Callahan, Shawn; Gressler, William; Thomas, Sandrine J.; Gessner, Chuck; Warner, Mike; Barr, Jeff; Lotz, Paul J.; Schumacher, German; Wiecha, Oliver; Angeli, George; Andrew, John; Claver, Chuck; Schoening, Bill; Sebag, Jacques; Krabbendam, Victor; Neill, Doug; Hileman, Ed; Muller, Gary; Araujo, Constanza; Orden Martinez, Alfredo; Perezagua Aguado, Manuel; García-Marchena, Luis; Ruiz de Argandoña, Ismael; Romero, Francisco M.; Rodríguez, Ricardo; Carlos González, José; Venturini, Marco

2016-08-01

This paper describes the status and details of the large synoptic survey telescope1,2,3 mount assembly (TMA). On June 9th, 2014 the contract for the design and build of the large synoptic survey telescope mount assembly (TMA) was awarded to GHESA Ingeniería y Tecnología, S.A. and Asturfeito, S.A. The design successfully passed the preliminary design review on October 2, 2015 and the final design review January 29, 2016. This paper describes the detailed design by subsystem, analytical model results, preparations being taken to complete the fabrication, and the transportation and installation plans to install the mount on Cerro Pachón in Chile. This large project is the culmination of work by many people and the authors would like to thank everyone that has contributed to the success of this project.

Status of the MiniCLEAN dark matter experiment

NASA Astrophysics Data System (ADS)

Rielage, Keith

2009-10-01

MiniCLEAN utilizes over 400 kg of liquid cryogen to detect nuclear recoils from WIMP dark matter with a projected sensitivity of 2x10-45 cm^2 for a mass of 100 GeV. The liquid cryogen is interchangeable between argon and neon to study the A^2 dependence of the potential signal and examine backgrounds. MiniCLEAN utilizes a unique modular design with spherical geometry to maximize the light yield using cold photomultiplier tubes in a single-phase detector. Pulse shape discrimination techniques are used to separate nuclear recoil signals from electron recoil backgrounds. Particular attention is being paid to mitigating the backgrounds from contamination of surfaces by radon daughters during assembly. The design and assembly status of the experiment will be discussed. The projected timeline and future plans for staging the experiment at SNOLAB in Sudbury, Canada will be presented.

Identification of optimum sequencing depth especially for de novo genome assembly of small genomes using next generation sequencing data.

PubMed

Desai, Aarti; Marwah, Veer Singh; Yadav, Akshay; Jha, Vineet; Dhaygude, Kishor; Bangar, Ujwala; Kulkarni, Vivek; Jere, Abhay

2013-01-01

Next Generation Sequencing (NGS) is a disruptive technology that has found widespread acceptance in the life sciences research community. The high throughput and low cost of sequencing has encouraged researchers to undertake ambitious genomic projects, especially in de novo genome sequencing. Currently, NGS systems generate sequence data as short reads and de novo genome assembly using these short reads is computationally very intensive. Due to lower cost of sequencing and higher throughput, NGS systems now provide the ability to sequence genomes at high depth. However, currently no report is available highlighting the impact of high sequence depth on genome assembly using real data sets and multiple assembly algorithms. Recently, some studies have evaluated the impact of sequence coverage, error rate and average read length on genome assembly using multiple assembly algorithms, however, these evaluations were performed using simulated datasets. One limitation of using simulated datasets is that variables such as error rates, read length and coverage which are known to impact genome assembly are carefully controlled. Hence, this study was undertaken to identify the minimum depth of sequencing required for de novo assembly for different sized genomes using graph based assembly algorithms and real datasets. Illumina reads for E.coli (4.6 MB) S.kudriavzevii (11.18 MB) and C.elegans (100 MB) were assembled using SOAPdenovo, Velvet, ABySS, Meraculous and IDBA-UD. Our analysis shows that 50X is the optimum read depth for assembling these genomes using all assemblers except Meraculous which requires 100X read depth. Moreover, our analysis shows that de novo assembly from 50X read data requires only 6-40 GB RAM depending on the genome size and assembly algorithm used. We believe that this information can be extremely valuable for researchers in designing experiments and multiplexing which will enable optimum utilization of sequencing as well as analysis resources.

Evaluating the iterative development of VR/AR human factors tools for manual work.

PubMed

Liston, Paul M; Kay, Alison; Cromie, Sam; Leva, Chiara; D'Cruz, Mirabelle; Patel, Harshada; Langley, Alyson; Sharples, Sarah; Aromaa, Susanna

2012-01-01

This paper outlines the approach taken to iteratively evaluate a set of VR/AR (virtual reality / augmented reality) applications for five different manual-work applications - terrestrial spacecraft assembly, assembly-line design, remote maintenance of trains, maintenance of nuclear reactors, and large-machine assembly process design - and examines the evaluation data for evidence of the effectiveness of the evaluation framework as well as the benefits to the development process of feedback from iterative evaluation. ManuVAR is an EU-funded research project that is working to develop an innovative technology platform and a framework to support high-value, high-knowledge manual work throughout the product lifecycle. The results of this study demonstrate the iterative improvements reached throughout the design cycles, observable through the trending of the quantitative results from three successive trials of the applications and the investigation of the qualitative interview findings. The paper discusses the limitations of evaluation in complex, multi-disciplinary development projects and finds evidence of the effectiveness of the use of the particular set of complementary evaluation methods incorporating a common inquiry structure used for the evaluation - particularly in facilitating triangulation of the data.

An 8.4-GHz dual-maser front-end system for Parkes reimplementation

NASA Technical Reports Server (NTRS)

Trowbridge, D. L.; Loreman, J. R.; Brunzie, T. J.; Quinn, R.

1990-01-01

An 8.4-GHz front-end system consisting of a feedhorn, a waveguide feed assembly, dual masers, and downconverters was reimplemented at Parkes as part of the Parkes Canberra Telemetry Array for the Voyager Neptune encounter. The front-end system was originally assembled by the European Space Agency and installed on the Parkes antenna for the Giotto project. It was also used on a time-sharing basis by the Deep Space Network as part of the Parkes Canberra Telemetry Array to enhance the data return from the Voyager Uranus encounter. At the conclusion of these projects in 1986, part of the system was then shipped to JPL on loan for reimplementation at Parkes for the Voyager Neptune encounter. New design and implementation required to make the system operable at Parkes included new microwave front-end control cabinets, closed-cycle refrigeration monitor system, noise-adding radiometer system, front-end controller assembly, X81 local oscillator multiplier, and refurbishment of the original dual 8.4-GHz traveling-wave masers and waveguide feed system. The front-end system met all requirements during the encounter and was disassembled in October 1989 and returned to JPL.

Kodak Mirror Assembly Tested at Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

2003-01-01

This photo (a frontal view) is of one of many segments of the Eastman-Kodak mirror assembly being tested for the James Webb Space Telescope (JWST) project at the X-Ray Calibration Facility at Marshall Space Flight Center (MSFC). MSFC is supporting Goddard Space Flight Center (GSFC) in developing the JWST by taking numerous measurements to predict its future performance. The tests are conducted in a vacuum chamber cooled to approximate the super cold temperatures found in space. During its 27 years of operation, the facility has performed testing in support of a wide array of projects, including the Hubble Space Telescope (HST), Solar A, Chandra technology development, Chandra High Resolution Mirror Assembly and science instruments, Constellation X-Ray Mission, and Solar X-Ray Imager, currently operating on a Geostationary Operational Environment Satellite. The JWST is NASA's next generation space telescope, a successor to the Hubble Space Telescope, named in honor of NASA's second administrator, James E. Webb. It is scheduled for launch in 2010 aboard an expendable launch vehicle. It will take about 3 months for the spacecraft to reach its destination, an orbit of 940,000 miles in space.

Kodak Mirror Assembly Tested at Marshall Space Flight Center

NASA Technical Reports Server (NTRS)

2003-01-01

This photo (a side view) is of one of many segments of the Eastman-Kodak mirror assembly being tested for the James Webb Space Telescope (JWST) project at the X-Ray Calibration Facility at Marshall Space Flight Center (MSFC). MSFC is supporting Goddard Space Flight Center (GSFC) in developing the JWST by taking numerous measurements to predict its future performance. The tests are conducted in a vacuum chamber cooled to approximate the super cold temperatures found in space. During its 27 years of operation, the facility has performed testing in support of a wide array of projects, including the Hubble Space Telescope (HST), Solar A, Chandra technology development, Chandra High Resolution Mirror Assembly and science instruments, Constellation X-Ray Mission, and Solar X-Ray Imager, currently operating on a Geostationary Operational Environment Satellite. The JWST is NASA's next generation space telescope, a successor to the Hubble Space Telescope, named in honor of NASA's second administrator, James E. Webb. It is scheduled for launch in 2010 aboard an expendable launch vehicle. It will take about 3 months for the spacecraft to reach its destination, an orbit of 940,000 miles in space.

Space Science

NASA Image and Video Library

2003-04-09

The Eastman-Kodak mirror assembly is being tested for the James Webb Space Telescope (JWST) project at the X-Ray Calibration Facility at Marshall Space Flight Center (MSFC). In this photo, an MSFC employee is inspecting one of many segments of the mirror assembly for flaws. MSFC is supporting Goddard Space Flight Center (GSFC) in developing the JWST by taking numerous measurements to predict its future performance. The tests are conducted in a vacuum chamber cooled to approximate the super cold temperatures found in space. During its 27 years of operation, the facility has performed testing in support of a wide array of projects, including the Hubble Space Telescope (HST), Solar A, Chandra technology development, Chandra High Resolution Mirror Assembly and science instruments, Constellation X-Ray Mission, and Solar X-Ray Imager, currently operating on a Geostationary Operational Environment Satellite. The JWST is NASA's next generation space telescope, a successor to the Hubble Space Telescope, named in honor of NASA's second administrator, James E. Webb. It is scheduled for launch in 2010 aboard an expendable launch vehicle. It will take about 3 months for the spacecraft to reach its destination, an orbit of 940,000 miles in space.

Space Science

NASA Image and Video Library

2003-04-09

The Eastman-Kodak mirror assembly is being tested for the James Webb Space Telescope (JWST) project at the X-Ray Calibration Facility at Marshall Space Flight Center (MSFC). In this photo, one of many segments of the mirror assembly is being set up inside the 24-ft vacuum chamber where it will undergo x-ray calibration tests. MSFC is supporting Goddard Space Flight Center (GSFC) in developing the JWST by taking numerous measurements to predict its future performance. The tests are conducted in a vacuum chamber cooled to approximate the super cold temperatures found in space. During its 27 years of operation, the facility has performed testing in support of a wide array of projects, including the Hubble Space Telescope (HST), Solar A, Chandra technology development, Chandra High Resolution Mirror Assembly and science instruments, Constellation X-Ray Mission, and Solar X-Ray Imager, currently operating on a Geostationary Operational Environment Satellite. The JWST is NASA's next generation space telescope, a successor to the Hubble Space Telescope, named in honor of NASA's second administrator, James E. Webb. It is scheduled for launch in 2010 aboard an expendable launch vehicle. It will take about 3 months for the spacecraft to reach its destination, an orbit of 940,000 miles in space.

Magnetic antenna using metallic glass

NASA Technical Reports Server (NTRS)

Desch, Michael D. (Inventor); Farrell, William M. (Inventor); Houser, Jeffrey G. (Inventor)

1996-01-01

A lightweight search-coil antenna or sensor assembly for detecting magnetic fields and including a multi-turn electromagnetic induction coil wound on a spool type coil form through which is inserted an elongated coil loading member comprised of metallic glass material wrapped around a dielectric rod. The dielectric rod consists of a plastic or a wooden dowel having a length which is relatively larger than its thickness so as to provide a large length-to-diameter ratio. A tri-axial configuration includes a housing in which is located three substantially identical mutually orthogonal electromagnetic induction coil assemblies of the type described above wherein each of the assemblies include an electromagnetic coil wound on a dielectric spool with an elongated metallic glass coil loading member projecting therethrough.

Processing and thermodynamics research, Volume II. Monthly progress report, November 1984

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

Data assembly is in progress on the Venezuelan Cerro Negro, California, Wilmington and Mexican Mayan crudes. A draft of a proposal is in preparation that might join this correlational work with complementary correlational work at the University of Oklahoma (Project BPT1). Cerro Negro 200 to 425/sup 0/C and 425 to 550/sup 0/C saturate fractions were analyzed with the CEC 21-103 mass spectrometer using ASTM method D2786, and experiments were devised to establish reproducibility of analyses with the KRATOS MS-50 (Project BPT2). Thermodynamic property measurements on organic nitrogen compounds included a liquid phase heat capacity study of 2,5-dimethylpyridine, and the oxygenmore » sensitivity of phenanthridine was checked prior to bomb calorimetry (Project BPT3A). Heat capacity measurements on chroman are progressing well, and vapor pressure measurements of tetrahydrophenanthrene are in progress (Project BPT3B). Work was begun on the design and procurement of equipment necessary for construction of a bench scale catalytic cracking unit. The supercritical extraction unit is being assembled (Project OPT1). Thiopene separations were begun on Cerro Negro 425 to 550/sup 0/C neutrals, and separation of sulfides from Cerro Negro 550 to 700/sup 0/C and 700+/sup 0/C neutrals was completed (Project OPT2). Preparations for PVT studies of methanol are nearing completion, and a list of critically needed PVT measurements was reviewed to help select the next compound for study. A possible candidate is hydrogen sulfide. (Project OPT3). Good progress continues in the treatment of the RP feed (Project OPT4) to recover Milspec F-76. It is anticipated that 85% recovery is possible by the chemical treatment/distillation process.« less

Effectiveness of high school safety belt instruction

DOT National Transportation Integrated Search

1982-12-01

The Effectiveness of High School Safety Belt Instruction was developed during a two-phased project. In Phase I, Focus Group Activities were conducted to determine whether audiovisual safety belt instructional materials assembled by the National Highw...

Test plan : I-40 TTIS tourist intercept survey

DOT National Transportation Integrated Search

1998-06-01

This document assembles best practices and presents practical advice on how to acquire the software components of Intelligent Transportation Systems (ITS). The intended audience is the "customers" - the project leaders, technical contract managers, d...

GIS Mapping of Environmental Justice Census Characteristics

DOT National Transportation Integrated Search

2004-10-01

As an entity utilizing federal funds, the Oregon Department of Transportation (ODOT) is responsible for successfully integrating environmental justice (EJ) into its program and planning activities. The objective of this project was to assemble a Geog...

Infrared Astronomical Satellite View of the Sky

NASA Image and Video Library

2009-11-03

Nearly the entire sky, as seen in infrared wavelengths and projected at one-half degree resolution, is shown in this image, assembled from six months of data from the NASA Infrared Astronomical Satellite, or IRAS.

Kent, Ohio traveler management coordination center (TMCC).

DOT National Transportation Integrated Search

2014-03-01

A project team consisting of the Portage Area Regional Transportation Authority (PARTA), Geauga County Transit, Trapeze Group, and : Kotting Consulting assembled a proposal to design a model system of human service transportation coordination using I...

The Chrysler-Weldtech Approach to Retraining Displaced Workers. A Model Project to Develop Specialized Welding Skills Required for Modern Auto Assembly Operations. Final Technical Report.

ERIC Educational Resources Information Center

Lyons, Thomas M.; Knight, Glen A.

A model project was conducted to demonstrate how Chrysler, in partnership with the education community and the government, could provide technical training to enable displaced workers to contribute to the "H-Body" car launch, to improve their job skills, and to enhance their future employability. The training was conducted on a pilot…

Operation SUN BEAM. Shot Small Boy, Project Officers’ Report. Project 2. 1. Initial Radiation Measurements

DTIC Science & Technology

1981-05-01

production 01 these gamma-rays and an experimental verification of their magnitude essential: 11) Tha transient radiation on electronics (TREE) work...Figure 2.6. It con- sisted of a scintillator, light pipe, photo sensitive device, and auxiliary electronic assembly. Arrangement of these elements in...types of mechanically interchangeable packages, consisting of a photosensitive device and auxiliary electronics , were available for each detector. (M

Optical mapping and its potential for large-scale sequencing projects.

PubMed

Aston, C; Mishra, B; Schwartz, D C

1999-07-01

Physical mapping has been rediscovered as an important component of large-scale sequencing projects. Restriction maps provide landmark sequences at defined intervals, and high-resolution restriction maps can be assembled from ensembles of single molecules by optical means. Such optical maps can be constructed from both large-insert clones and genomic DNA, and are used as a scaffold for accurately aligning sequence contigs generated by shotgun sequencing.

LSSA (Low-cost Silicon Solar Array) project

NASA Technical Reports Server (NTRS)

1976-01-01

Methods are explored for economically generating electrical power to meet future requirements. The Low-Cost Silicon Solar Array Project (LSSA) was established to reduce the price of solar arrays by improving manufacturing technology, adapting mass production techniques, and promoting user acceptance. The new manufacturing technology includes the consideration of new silicon refinement processes, silicon sheet growth techniques, encapsulants, and automated assembly production being developed under contract by industries and universities.

Orthotic devices using lightweight composite materials

NASA Technical Reports Server (NTRS)

Harrison, E., Jr.

1983-01-01

Potential applications of high strength, lightweight composite technology in the orthotic field were studied. Several devices were designed and fabricated using graphite-epoxy composite technology. Devices included shoe plates, assistive walker devices, and a Simes prosthesis reinforcement. Several other projects having medical application were investigated and evaluations were made of the potential for use of composite technology. A seat assembly was fabricated using sandwich construction techniques for the Total Wheelchair Project.

Haplotype assembly in polyploid genomes and identical by descent shared tracts.

PubMed

Aguiar, Derek; Istrail, Sorin

2013-07-01

Genome-wide haplotype reconstruction from sequence data, or haplotype assembly, is at the center of major challenges in molecular biology and life sciences. For complex eukaryotic organisms like humans, the genome is vast and the population samples are growing so rapidly that algorithms processing high-throughput sequencing data must scale favorably in terms of both accuracy and computational efficiency. Furthermore, current models and methodologies for haplotype assembly (i) do not consider individuals sharing haplotypes jointly, which reduces the size and accuracy of assembled haplotypes, and (ii) are unable to model genomes having more than two sets of homologous chromosomes (polyploidy). Polyploid organisms are increasingly becoming the target of many research groups interested in the genomics of disease, phylogenetics, botany and evolution but there is an absence of theory and methods for polyploid haplotype reconstruction. In this work, we present a number of results, extensions and generalizations of compass graphs and our HapCompass framework. We prove the theoretical complexity of two haplotype assembly optimizations, thereby motivating the use of heuristics. Furthermore, we present graph theory-based algorithms for the problem of haplotype assembly using our previously developed HapCompass framework for (i) novel implementations of haplotype assembly optimizations (minimum error correction), (ii) assembly of a pair of individuals sharing a haplotype tract identical by descent and (iii) assembly of polyploid genomes. We evaluate our methods on 1000 Genomes Project, Pacific Biosciences and simulated sequence data. HapCompass is available for download at http://www.brown.edu/Research/Istrail_Lab/. Supplementary data are available at Bioinformatics online.

77 FR 5817 - Ocean Renewable Power Company, Tidal Energy Project, Cobscook Bay, ME

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-06

... ocean floor, used for generating electricity from tidal currents and is now providing the public the... impacts associated with installation of an underwater cable assembly to transfer electricity to a power...

EU-US standards harmonization task group report : status of ITS security standards.

DOT National Transportation Integrated Search

1998-07-01

This document assembles best practices and presents practical advice on how to acquire the software components of Intelligent Transportation Systems (ITS). The intended audience is the customers--project leaders, technical contract managers, de...

Detail, rear door types, building 242, oblique view to southwest, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail, rear door types, building 242, oblique view to southwest, 90 mm lens. - Travis Air Force Base, Nuclear Weapons Assembly Building, W Street, Armed Forces Special Weapons Project Q Area, Fairfield, Solano County, CA

KENNEDY SPACE CENTER, FLA. - Jim Comer, United Space Alliance project leader for Columbia reconstruction, speaks to members of the Columbia Reconstruction Team during transfer of debris from the Columbia Debris Hangar to its permanent storage site in the Vehicle Assembly Building. More than 83,000 pieces of debris were shipped to KSC during search and recovery efforts in East Texas. That represents about 38 percent of the dry weight of Columbia, equaling almost 85,000 pounds.

NASA Image and Video Library

2003-09-15

KENNEDY SPACE CENTER, FLA. - Jim Comer, United Space Alliance project leader for Columbia reconstruction, speaks to members of the Columbia Reconstruction Team during transfer of debris from the Columbia Debris Hangar to its permanent storage site in the Vehicle Assembly Building. More than 83,000 pieces of debris were shipped to KSC during search and recovery efforts in East Texas. That represents about 38 percent of the dry weight of Columbia, equaling almost 85,000 pounds.

Freedom is an international partnership. [foreign contributions to NASA Space Station project

NASA Technical Reports Server (NTRS)

Kohrs, Richard H.

1990-01-01

The NASA Space Station Freedom (SSF) project initiated in 1984 is a collaborative one among the U.S., Japan, Canada, and the 10 nations participating in ESA. The SSF partners have over the last six years defined user requirements, decided on the hardware to be manufactured, and constructed a framework for long-term cooperation. SSF will be composed of user elements furnished by the foreign partners and a U.S.-supplied infrastructure encompassing the truss assembly, electrical power system, and crew living quarters. The U.S. will also furnish a lab and a polar-orbit platform; ESA, a second lab and the coorbiting Free-Flying Laboratory, as well as a second polar platform. Japan's Japanese Experiment Module shall include an Exposed Facility and an Experimental Logistics module. Canada will contribute the Mobile Servicing System robotic assembler/maintainer for the whole of SFF.

Haemonchus contortus: Genome Structure, Organization and Comparative Genomics.

PubMed

Laing, R; Martinelli, A; Tracey, A; Holroyd, N; Gilleard, J S; Cotton, J A

2016-01-01

One of the first genome sequencing projects for a parasitic nematode was that for Haemonchus contortus. The open access data from the Wellcome Trust Sanger Institute provided a valuable early resource for the research community, particularly for the identification of specific genes and genetic markers. Later, a second sequencing project was initiated by the University of Melbourne, and the two draft genome sequences for H. contortus were published back-to-back in 2013. There is a pressing need for long-range genomic information for genetic mapping, population genetics and functional genomic studies, so we are continuing to improve the Wellcome Trust Sanger Institute assembly to provide a finished reference genome for H. contortus. This review describes this process, compares the H. contortus genome assemblies with draft genomes from other members of the strongylid group and discusses future directions for parasite genomics using the H. contortus model. Copyright © 2016 Elsevier Ltd. All rights reserved.

A translator writing system for microcomputer high-level languages and assemblers

NASA Technical Reports Server (NTRS)

Collins, W. R.; Knight, J. C.; Noonan, R. E.

1980-01-01

In order to implement high level languages whenever possible, a translator writing system of advanced design was developed. It is intended for routine production use by many programmers working on different projects. As well as a fairly conventional parser generator, it includes a system for the rapid generation of table driven code generators. The parser generator was developed from a prototype version. The translator writing system includes various tools for the management of the source text of a compiler under construction. In addition, it supplies various default source code sections so that its output is always compilable and executable. The system thereby encourages iterative enhancement as a development methodology by ensuring an executable program from the earliest stages of a compiler development project. The translator writing system includes PASCAL/48 compiler, three assemblers, and two compilers for a subset of HAL/S.

Spent nuclear fuel assembly inspection using neutron computed tomography

NASA Astrophysics Data System (ADS)

Pope, Chad Lee

The research presented here focuses on spent nuclear fuel assembly inspection using neutron computed tomography. Experimental measurements involving neutron beam transmission through a spent nuclear fuel assembly serve as benchmark measurements for an MCNP simulation model. Comparison of measured results to simulation results shows good agreement. Generation of tomography images from MCNP tally results was accomplished using adapted versions of built in MATLAB algorithms. Multiple fuel assembly models were examined to provide a broad set of conclusions. Tomography images revealing assembly geometric information including the fuel element lattice structure and missing elements can be obtained using high energy neutrons. A projection difference technique was developed which reveals the substitution of unirradiated fuel elements for irradiated fuel elements, using high energy neutrons. More subtle material differences such as altering the burnup of individual elements can be identified with lower energy neutrons provided the scattered neutron contribution to the image is limited. The research results show that neutron computed tomography can be used to inspect spent nuclear fuel assemblies for the purpose of identifying anomalies such as missing elements or substituted elements. The ability to identify anomalies in spent fuel assemblies can be used to deter diversion of material by increasing the risk of early detection as well as improve reprocessing facility operations by confirming the spent fuel configuration is as expected or allowing segregation if anomalies are detected.

Arctic communications techniques: Remote unattended power systems

NASA Astrophysics Data System (ADS)

Walker, G.

1986-02-01

The purpose of this report is to describe the accomplishments during the reporting period, 16 December 1985 through 1 February 1986, on the project entitled Arctic Communications Techniques: Remote Unattended Power Systems. All of the fabricated component parts for the first Ross-Stirling engine were completed. During the assembly process several interferences between some of the parts in the rotating mechanism were discovered causing drawing changes and subsequent rework to a few of the components. Assembly of the first engine was then completed. On the first attempt the engine ran successfully at approximately 3500 rpm.

Beam shaping assembly optimization for (7)Li(p,n)(7)Be accelerator based BNCT.

PubMed

Minsky, D M; Kreiner, A J

2014-06-01

Within the framework of accelerator-based BNCT, a project to develop a folded Tandem-ElectroStatic-Quadrupole accelerator is under way at the Atomic Energy Commission of Argentina. The proposed accelerator is conceived to deliver a proton beam of 30mA at about 2.5MeV. In this work we explore a Beam Shaping Assembly (BSA) design based on the (7)Li(p,n)(7)Be neutron production reaction to obtain neutron beams to treat deep seated tumors. © 2013 Elsevier Ltd. All rights reserved.

Moisture Management for High R-Value Walls

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lepage, R.; Schumacher, C.; Lukachko, A.

2013-11-01

This report explains the moisture-related concerns for high R-value wall assemblies and discusses past Building America research work that informs this study. In this project, hygrothermal simulations were prepared for several common approaches to High R-value wall construction in six cities (Houston, Atlanta, Seattle, St. Louis, Chicago, and International Falls) representing a range of climate zones. The modeling program assessed the moisture durability of the wall assemblies based on three primary sources of moisture: construction moisture, air leakage condensation, and bulk water leakage; the report presents results of the study.

Leadership Development Program Final Project

NASA Technical Reports Server (NTRS)

Parrish, Teresa C.

2016-01-01

TOSC is NASA's prime contractor tasked to successfully assemble, test, and launch the EM1 spacecraft. TOSC success is highly dependent on design products from the other NASA Programs manufacturing and delivering the flight hardware; Space Launch System(SLS) and Multi-Purpose Crew Vehicle(MPCV). Design products directly feed into TOSC's: Procedures, Personnel training, Hardware assembly, Software development, Integrated vehicle test and checkout, Launch. TOSC senior management recognized a significant schedule risk as these products are still being developed by the other two (2) programs; SVE and ACE positions were created.

Automated Array Assembly, Phase 2

NASA Technical Reports Server (NTRS)

Carbajal, B. G.

1979-01-01

The Automated Array Assembly Task, Phase 2 of the Low Cost Silicon Solar Array Project is a process development task. The contract provides for the fabrication of modules from large area tandem junction cells (TJC). During this quarter, effort was focused on the design of a large area, approximately 36 sq cm, TJC and process verification runs. The large area TJC design was optimized for minimum I squared R power losses. In the TJM activity, the cell-module interfaces were defined, module substrates were formed and heat treated and clad metal interconnect strips were fabricated.

KSC-99pp1405

NASA Image and Video Library

1999-12-13

KENNEDY SPACE CENTER, Fla. -- The doors of the Vehicle Assembly Building (VAB) are open for the transfer of Space Shuttle Endeavour, on its mobile launcher platform, to Launch Pad 39A for mission STS-99. Named the Shuttle Radar Topography Mission (SRTM), it involves an international project spearheaded by the National Imagery and Mapping Agency and NASA, with participation of the German Aerospace Center DLR. The SRTM consists of a specially modified radar system that will gather data for the most accurate and complete topographic map of the Earth's surface that has ever been assembled

Problem-Solving Environments (PSEs) to Support Innovation Clustering

NASA Technical Reports Server (NTRS)

Gill, Zann

1999-01-01

This paper argues that there is need for high level concepts to inform the development of Problem-Solving Environment (PSE) capability. A traditional approach to PSE implementation is to: (1) assemble a collection of tools; (2) integrate the tools; and (3) assume that collaborative work begins after the PSE is assembled. I argue for the need to start from the opposite premise, that promoting human collaboration and observing that process comes first, followed by the development of supporting tools, and finally evolution of PSE capability through input from collaborating project teams.

Measure Guideline: Incorporating Thick Layers of Exterior Rigid Insulation on Walls

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lstiburek, Joseph; Baker, Peter

This measure guideline provides information about the design and construction of wall assemblies that use layers of rigid exterior insulation thicker than 1-½ inches and that require a secondary cladding attachment location exterior to the insulation. The guideline is separated into several distinct sections that cover: fundamental building science principles relating to the use of exterior insulation on wall assemblies; design principles for tailoring this use to the specific project goals and requirements; and construction detailing to increase understanding about implementing the various design elements.

Advanced Space Transportation Program (ASTP)

NASA Image and Video Library

1997-08-07

This double exposure depicts Marshall Space Flight Center's (MSFC) Test Stand 116 hosting a 60K Bantam Fastrac thrust chamber assembly test. The lower right exposure shows the engine firing in the test stand while the center exposure reveals workers monitoring the test in the interior block house of the test facility. The thrust chamber assembly is only part of the Fastrac engine project to build a low-cost engine for the X-34, an alternate light-weight unmarned launch vehicle. Both the nozzle and the engine for Fastrac are being manufactured at MSFC.

Integrated automation for manufacturing of electronic assemblies

NASA Technical Reports Server (NTRS)

Sampite, T. Joseph

1991-01-01

Since 1985, the Naval Ocean Systems Center has been identifying and developing needed technology for flexible manufacturing of hybrid microelectronic assemblies. Specific projects have been accomplished through contracts with manufacturing companies, equipment suppliers, and joint efforts with other government agencies. The resulting technology has been shared through semi-annual meetings with government, industry, and academic representatives who form an ad hoc advisory panel. More than 70 major technical capabilities have been identified for which new development is needed. Several of these developments have been completed and are being shared with industry.

Approach to the E-ELT dome and main structure challenges

NASA Astrophysics Data System (ADS)

Bilbao, Armando; Murga, Gaizka; Gómez, Celia; Llarena, Javier

2014-07-01

The E-ELT as a whole could be classified as an extremely challenging project. More precisely, it should be defined as an array of many different sub-challenges, which comprise technical, logistical and managerial matters. This paper reviews some of these critical challenges, in particular those related to the Dome and the Main Structure, suggesting ways to face them in the most pragmatic way possible. Technical challenges for the Dome and the Main Structure are mainly related to the need to upscale current design standards to an order of magnitude larger design. Trying a direct design escalation is not feasible; it would not work. A design effort is needed to cross hybridize current design standards with technologies coming from other different applications. Innovative design is therefore not a wish but a must. And innovative design comes along with design risk. Design risk needs to be tackled from two angles: on the one hand through thorough design validation analysis and on the other hand through extensive pre-assembly and testing. And, once again, full scale integrated pre-assembly and testing of extremely large subsystems is not always possible. Therefore, defining a comprehensive test plan for critical components, critical subsystems and critical subassemblies becomes essential. Logistical challenges are linked to the erection site. Cerro Armazones is a remote site and this needs to be considered when evaluating transport and erection requirements. But it is not only the remoteness of the site that needs to be considered. The size of both Dome and Main Structure require large construction cranes and a well defined erection plan taking into account pre-assembly strategies, limited plan area utilization, erection sequence, erection stability during intermediate stages and, very specifically, efficient coordination between the Dome and the Main Structure erection processes. Managerial issues pose another set of challenges in this project. Both the size of the project and its special technical characteristics require specific managerial skills. Due to the size of the project it becomes essential to effectively manage and integrate a large number of suppliers and fabricators, of very different nature and geographically distributed. Project management plans need to cope with this situation. Also, extensive on site activities require intensive on site organization in line with large construction management strategies. Finally, the technical edge of the project requires deep technical understanding at management level in order to be able to take sound strategic decisions throughout the project in terms of the overall project quality, cost and schedule.

Interpreting Microbial Biosynthesis in the Genomic Age: Biological and Practical Considerations

PubMed Central

Miller, Ian J.; Chevrette, Marc G.; Kwan, Jason C.

2017-01-01

Genome mining has become an increasingly powerful, scalable, and economically accessible tool for the study of natural product biosynthesis and drug discovery. However, there remain important biological and practical problems that can complicate or obscure biosynthetic analysis in genomic and metagenomic sequencing projects. Here, we focus on limitations of available technology as well as computational and experimental strategies to overcome them. We review the unique challenges and approaches in the study of symbiotic and uncultured systems, as well as those associated with biosynthetic gene cluster (BGC) assembly and product prediction. Finally, to explore sequencing parameters that affect the recovery and contiguity of large and repetitive BGCs assembled de novo, we simulate Illumina and PacBio sequencing of the Salinispora tropica genome focusing on assembly of the salinilactam (slm) BGC. PMID:28587290

Enhanced tamper indicator

DOEpatents

Garcia, Anthony R.; Johnston, Roger G.

2003-07-08

The present invention provides an apparatus and method whereby the reliability and tamper-resistance of tamper indicators can be improved. A flexible connector may be routed through a latch for an enclosure such as a door or container, and the free ends of the flexible connector may be passed through a first locking member and firmly attached to an insert through the use of one or more attachment members such as set screws. A second locking member may then be assembled in interlocking relation with the first locking member to form an interlocked assembly around the insert. The insert may have one or more sharp projections extending toward the first or second locking member so that any compressive force applied in an attempt to disassemble the interlocked assembly results in permanent, visible damage to the first or second locking member.

New tool to assemble repetitive regions using next-generation sequencing data

NASA Astrophysics Data System (ADS)

Kuśmirek, Wiktor; Nowak, Robert M.; Neumann, Łukasz

2017-08-01

The next generation sequencing techniques produce a large amount of sequencing data. Some part of the genome are composed of repetitive DNA sequences, which are very problematic for the existing genome assemblers. We propose a modification of the algorithm for a DNA assembly, which uses the relative frequency of reads to properly reconstruct repetitive sequences. The new approach was implemented and tested, as a demonstration of the capability of our software we present some results for model organisms. The new implementation, using a three-layer software architecture was selected, where the presentation layer, data processing layer, and data storage layer were kept separate. Source code as well as demo application with web interface and the additional data are available at project web-page: http://dnaasm.sourceforge.net.

The Past, Present, and Future of Human Centromere Genomics

PubMed Central

Aldrup-MacDonald, Megan E.; Sullivan, Beth A.

2014-01-01

The centromere is the chromosomal locus essential for chromosome inheritance and genome stability. Human centromeres are located at repetitive alpha satellite DNA arrays that compose approximately 5% of the genome. Contiguous alpha satellite DNA sequence is absent from the assembled reference genome, limiting current understanding of centromere organization and function. Here, we review the progress in centromere genomics spanning the discovery of the sequence to its molecular characterization and the work done during the Human Genome Project era to elucidate alpha satellite structure and sequence variation. We discuss exciting recent advances in alpha satellite sequence assembly that have provided important insight into the abundance and complex organization of this sequence on human chromosomes. In light of these new findings, we offer perspectives for future studies of human centromere assembly and function. PMID:24683489

A Hybrid Parallel Strategy Based on String Graph Theory to Improve De Novo DNA Assembly on the TianHe-2 Supercomputer.

PubMed

Zhang, Feng; Liao, Xiangke; Peng, Shaoliang; Cui, Yingbo; Wang, Bingqiang; Zhu, Xiaoqian; Liu, Jie

2016-06-01

' The de novo assembly of DNA sequences is increasingly important for biological researches in the genomic era. After more than one decade since the Human Genome Project, some challenges still exist and new solutions are being explored to improve de novo assembly of genomes. String graph assembler (SGA), based on the string graph theory, is a new method/tool developed to address the challenges. In this paper, based on an in-depth analysis of SGA we prove that the SGA-based sequence de novo assembly is an NP-complete problem. According to our analysis, SGA outperforms other similar methods/tools in memory consumption, but costs much more time, of which 60-70 % is spent on the index construction. Upon this analysis, we introduce a hybrid parallel optimization algorithm and implement this algorithm in the TianHe-2's parallel framework. Simulations are performed with different datasets. For data of small size the optimized solution is 3.06 times faster than before, and for data of middle size it's 1.60 times. The results demonstrate an evident performance improvement, with the linear scalability for parallel FM-index construction. This results thus contribute significantly to improving the efficiency of de novo assembly of DNA sequences.

Calculated Drag of an Aerial Refueling Assembly Through Airplane Performance Analysis

NASA Technical Reports Server (NTRS)

Vachon, Michael Jacob; Ray, Ronald J.

2004-01-01

The aerodynamic drag of an aerial refueling assembly was calculated during the Automated Aerial Refueling project at the NASA Dryden Flight Research Center. An F/A-18A airplane was specially instrumented to obtain accurate fuel flow measurements and to determine engine thrust. A standard Navy air refueling store with a retractable refueling hose and paradrogue was mounted to the centerline pylon of the F/A-18A airplane. As the paradrogue assembly was deployed and stowed, changes in the calculated thrust of the airplane occurred and were equated to changes in vehicle drag. These drag changes were attributable to the drag of the paradrogue assembly. The drag of the paradrogue assembly was determined to range from 200 to 450 lbf at airspeeds from 170 to 250 KIAS. Analysis of the drag data resulted in a single drag coefficient of 0.0056 for the paradrogue assembly that adequately matched the calculated drag for all flight conditions. The drag relief provided to the tanker airplane when a receiver airplane engaged the paradrogue is also documented from 35 to 270 lbf at the various flight conditions tested. The results support the development of accurate aerodynamic models to be used in refueling simulations and control laws for fully autonomous refueling.

Feasibility of using Big Area Additive Manufacturing to Directly Manufacture Boat Molds

DOE Office of Scientific and Technical Information (OSTI.GOV)

Post, Brian K.; Chesser, Phillip C.; Lind, Randall F.

The goal of this project was to explore the feasibility of using Big Area Additive Manufacturing (BAAM) to directly manufacture a boat mold without the need for coatings. All prior tooling projects with BAAM required the use to thick coatings to overcome the surface finish limitations of the BAAM process. While the BAAM process significantly lowers the cost of building the mold, the high cost element rapidly became the coatings (cost of the material, labor on coating, and finishing). As an example, the time and cost to manufacture the molds for the Wind Turbine project with TPI Composites Inc. andmore » the molds for the submarine project with Carderock Naval Warfare Systems was a fraction of the time and cost of the coatings. For this project, a catamaran boat hull mold was designed, manufactured, and assembled with an additional 0.15” thickness of material on all mold surfaces. After printing, the mold was immediately machined and assembled. Alliance MG, LLC (AMG), the industry partner of this project, experimented with mold release agents on the carbon-fiber reinforced acrylonitrile butadiene styrene (CF ABS) to verify that the material can be directly used as a mold (rather than needing a coating). In addition, for large molds (such as the wind turbine mold with TPI Composites Inc.), the mold only provided the target surface. A steel subframe had to be manufactured to provide structural integrity. If successful, this will significantly reduce the time and cost necessary for manufacturing large resin infusion molds using the BAAM process.« less

Rapid qualification of CSP assemblies by increase of ramp rates and cycling temperature ranges

NASA Technical Reports Server (NTRS)

Ghaffarian, R.; Kim, N.; Rose, D.; Hunter, B.; Devitt, K.; Long, T.

2001-01-01

Team members representing government agencies and private companies have joined together to pool in-kind resources for developing the quality and reliability of chip scale packages (CSPs) for a variety of projects.

EU-US standards harmonization task group report : feedback to standards development organizations - security

DOT National Transportation Integrated Search

1998-07-01

This document assembles best practices and presents practical advice on how to acquire the software components of Intelligent Transportation Systems (ITS). The intended audience is the customers--project leaders, technical contract managers, de...

ASSEMBLING DEM FILES FOR WATERSHED ANALYSIS. (R826595)

EPA Science Inventory

The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

Building 932, oblique view to northwest, 90 mm lens. Building ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Building 932, oblique view to northwest, 90 mm lens. Building 933-935 at extreme left. - Travis Air Force Base, Nuclear Weapons Assembly Plant 5, W Street, Armed Forces Special Weapons Project Q Area, Fairfield, Solano County, CA

GPHS-RTG's in support of the Cassini mission

NASA Astrophysics Data System (ADS)

1993-10-01

The following tasks were reported: Spacecraft integration and liaison; engineering support; safety; qualified unicouple fabrication; ETG fabrication/assembly/test; ground support equipment; RTG shipping and launch support; designs/reviews/mission applications; and project management/quality assurance/contract changes.

41. LOCK AND DAM NO. 26 (REPLACEMENT). LOCK LOCK ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

41. LOCK AND DAM NO. 26 (REPLACEMENT). LOCK -- LOCK GATES -- LIFT GATE, GATE LEAVES -- GENERAL ASSEMBLY. M-L 26(R) 21/28 - Upper Mississippi River 9-Foot Channel Project, Lock & Dam 26R, Alton, Madison County, IL

An Extensible Model and Analysis Framework

DTIC Science & Technology

2010-11-01

Eclipse or Netbeans Rich Client Platform (RCP). We call this the Triquetrum Project. Configuration files support narrower variability than Triquetrum/RCP...Triquetrum/RCP supports assembling in arbitrary ways. (12/08 presentation) 2. Prototyped OSGi component architecture for use with Netbeans and

Advanced Mirror Technology Development

NASA Technical Reports Server (NTRS)

Stahl, H. Philip

2017-01-01

The Advanced Mirror Technology Development (AMTD) project matures critical technologies required to enable ultra-stable 4-m-or-larger monolithic or segmented ultraviolet, optical, and infrared (UVOIR) space telescope primary-mirror assemblies for general astrophysics and ultra-high-contrast observations of exoplanets.

Reliability of CGA/LGA/HDI Package Board/Assembly (Revision A)

NASA Technical Reports Server (NTRS)

Ghaffarian, Reza

2013-01-01

This follow-up report presents reliability test results conducted by thermal cycling of five CGA assemblies evaluated under two extreme cycle profiles, representative of use for high-reliability applications. The thermal cycles ranged from a low temperature of 55 C to maximum temperatures of either 100 C or 125 C with slow ramp-up rate (3 C/min) and dwell times of about 15 minutes at the two extremes. Optical photomicrographs that illustrate key inspection findings of up to 200 thermal cycles are presented. Other information presented include an evaluation of the integrity of capacitors on CGA substrate after thermal cycling as well as process evaluation for direct assembly of an LGA onto PCB. The qualification guidelines, which are based on the test results for CGA/LGA/HDI packages and board assemblies, will facilitate NASA projects' use of very dense and newly available FPGA area array packages with known reliably and mitigation risks, allowing greater processing power in a smaller board footprint and lower system weight.

Bambus 2: scaffolding metagenomes.

PubMed

Koren, Sergey; Treangen, Todd J; Pop, Mihai

2011-11-01

Sequencing projects increasingly target samples from non-clonal sources. In particular, metagenomics has enabled scientists to begin to characterize the structure of microbial communities. The software tools developed for assembling and analyzing sequencing data for clonal organisms are, however, unable to adequately process data derived from non-clonal sources. We present a new scaffolder, Bambus 2, to address some of the challenges encountered when analyzing metagenomes. Our approach relies on a combination of a novel method for detecting genomic repeats and algorithms that analyze assembly graphs to identify biologically meaningful genomic variants. We compare our software to current assemblers using simulated and real data. We demonstrate that the repeat detection algorithms have higher sensitivity than current approaches without sacrificing specificity. In metagenomic datasets, the scaffolder avoids false joins between distantly related organisms while obtaining long-range contiguity. Bambus 2 represents a first step toward automated metagenomic assembly. Bambus 2 is open source and available from http://amos.sf.net. mpop@umiacs.umd.edu. Supplementary data are available at Bioinformatics online.

Bambus 2: scaffolding metagenomes

PubMed Central

Koren, Sergey; Treangen, Todd J.; Pop, Mihai

2011-01-01

Motivation: Sequencing projects increasingly target samples from non-clonal sources. In particular, metagenomics has enabled scientists to begin to characterize the structure of microbial communities. The software tools developed for assembling and analyzing sequencing data for clonal organisms are, however, unable to adequately process data derived from non-clonal sources. Results: We present a new scaffolder, Bambus 2, to address some of the challenges encountered when analyzing metagenomes. Our approach relies on a combination of a novel method for detecting genomic repeats and algorithms that analyze assembly graphs to identify biologically meaningful genomic variants. We compare our software to current assemblers using simulated and real data. We demonstrate that the repeat detection algorithms have higher sensitivity than current approaches without sacrificing specificity. In metagenomic datasets, the scaffolder avoids false joins between distantly related organisms while obtaining long-range contiguity. Bambus 2 represents a first step toward automated metagenomic assembly. Availability: Bambus 2 is open source and available from http://amos.sf.net. Contact: mpop@umiacs.umd.edu Supplementary Information: Supplementary data are available at Bioinformatics online. PMID:21926123

Blueprints for green biotech: development and application of standards for plant synthetic biology.

PubMed

Patron, Nicola J

2016-06-15

Synthetic biology aims to apply engineering principles to the design and modification of biological systems and to the construction of biological parts and devices. The ability to programme cells by providing new instructions written in DNA is a foundational technology of the field. Large-scale de novo DNA synthesis has accelerated synthetic biology by offering custom-made molecules at ever decreasing costs. However, for large fragments and for experiments in which libraries of DNA sequences are assembled in different combinations, assembly in the laboratory is still desirable. Biological assembly standards allow DNA parts, even those from multiple laboratories and experiments, to be assembled together using the same reagents and protocols. The adoption of such standards for plant synthetic biology has been cohesive for the plant science community, facilitating the application of genome editing technologies to plant systems and streamlining progress in large-scale, multi-laboratory bioengineering projects. © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society.

Architecture of the pontin/reptin complex, essential in the assembly of several macromolecular complexes

PubMed Central

Torreira, Eva; Jha, Sudhakar; López-Blanco, José R.; Arias-Palomo, Ernesto; Chacón, Pablo; Cañas, Cristina; Ayora, Sylvia; Dutta, Anindya; Llorca, Oscar

2008-01-01

Summary Pontin and reptin belong to the AAA+ family and they are essential for the structural integrity and catalytic activity of several chromatin remodeling complexes. They are also indispensable for the assembly of several ribonucleoprotein complexes, including telomerase. Here, we propose a structural model of the yeast pontin/reptin complex based on a cryo-electron microscopy reconstruction at 13 Å. Pontin/reptin hetero-dodecamers were purified from in vivo assembled complexes forming a double ring. Two rings interact through flexible domains projecting from each hexamer, constituting an atypical asymmetric form of oligomerization. These flexible domains and the AAA+ cores reveal significant conformational changes when compared to the crystal structure of human pontin that generate enlarged channels. This structure of endogenously assembled pontin/reptin complexes is different to previously described structures, suggesting that pontin and reptin could acquire distinct structural states to regulate their broad functions as molecular motors and scaffolds for nucleic acids and proteins. PMID:18940606

Distinct Mechanisms for Synchronization and Temporal Patterning of Odor-Encoding Neural Assemblies

NASA Astrophysics Data System (ADS)

MacLeod, Katrina; Laurent, Gilles

1996-11-01

Stimulus-evoked oscillatory synchronization of neural assemblies and temporal patterns of neuronal activity have been observed in many sensory systems, such as the visual and auditory cortices of mammals or the olfactory system of insects. In the locust olfactory system, single odor puffs cause the immediate formation of odor-specific neural assemblies, defined both by their transient synchronized firing and their progressive transformation over the course of a response. The application of an antagonist of ionotropic γ-aminobutyric acid (GABA) receptors to the first olfactory relay neuropil selectively blocked the fast inhibitory synapse between local and projection neurons. This manipulation abolished the synchronization of the odor-coding neural ensembles but did not affect each neuron's temporal response patterns to odors, even when these patterns contained periods of inhibition. Fast GABA-mediated inhibition, therefore, appears to underlie neuronal synchronization but not response tuning in this olfactory system. The selective desynchronization of stimulus-evoked oscillating neural assemblies in vivo is now possible, enabling direct functional tests of their significance for sensation and perception.

An Approach to the Articulation and Coordination of Occupational Preparatory Curriculums from the High School Through the Community College; Paper and Reports of Task Forces I and II.

ERIC Educational Resources Information Center

Oregon State Dept. of Employment, Salem.

The primary objective of this project was to produce a suggested plan for promoting and guiding the development and expansion of occupational education in Oregon high schools and community colleges. To achieve the major objective, special task force groups were assembled to work on specific aspects of the total project: labor market data,…

Anisotropic formation mechanism and nanomechanics for the self-assembly process of cross-β peptides

NASA Astrophysics Data System (ADS)

Deng, Li; Zhao, Yurong; Zhou, Peng; Xu, Hai; Wang, Yanting

2017-12-01

Not Available Project supported by the National Basic Research Program of China (Grant No. 2013CB932804), the National Natural Science Foundation of China (Grant Nos. 11421063, 11647601, 11504431, and 21503275), the Scientific Research Foundation of China University of Petroleum (East China) for Young Scholar (Grant Y1304073). YantingWang also thanks the financial support through the CAS Biophysics Interdisciplinary Innovation Team Project (Grant No. 2060299).

Understanding the Function of Tuberous Sclerosis Complex Genes in Neural Development: Roles in Synapse Assembly and Axon Guidance

DTIC Science & Technology

2012-02-01

The goal of our project was to use the fruitfly Drosophila melanogaster , to identify molecular mechanisms affecting nervous...includes tuberous sclerosis 1 and 2 (TSC1 and TSC2). This pathway is fully represented in the fruitfly Drosophila melanogaster and we took advantage...provided in the Appendix. 8 KEY RESEARCH ACCOMPLISHMENTS:  The goal of our project was to use the fruitfly Drosophila melanogaster ,

Design considerations for a servo optical projection system

NASA Astrophysics Data System (ADS)

Nadalsky, Michael; Allen, Daniel; Bien, Joseph

1987-01-01

The present servooptical projection system (SOPS) furnishes 'out-the-window' scenery for a pilot-training flight simulator; attention is given to the parametric tradeoffs made in the SOPS' optical design, as well as to its mechanical packaging and the servonetwork performance of the unit as integrated into a research/training helicopter flight simulator. The final SOPS configuration is a function of scan head design, assembly modularity, image deterioration method, and focal lengths and relative apertures.

ENGINEERED NEAR SURFACE DISPOSAL FACILITY OF THE INDUSTRIAL COMPLEX FOR SOLID RADWASTE MANAGEMENT AT CHERNOBYL NUCLEAR POWER PLANT

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ziehm, Ronny; Pichurin, Sergey Grigorevich

2003-02-27

As a part of the turnkey project ''Industrial Complex for Solid Radwaste Management (ICSRM) at the Chernobyl Nuclear Power Plant (ChNPP)'' an Engineered Near Surface Disposal Facility (ENSDF, LOT 3) will be built on the VEKTOR site within the 30 km Exclusion Zone of the ChNPP. This will be performed by RWE NUKEM GmbH, Germany, and it governs the design, licensing support, fabrication, assembly, testing, inspection, delivery, erection, installation and commissioning of the ENSDF. The ENSDF will receive low to intermediate level, short lived, processed/conditioned wastes from the ICSRM Solid Waste Processing Facility (SWPF, LOT 2), the ChNPP Liquid Radwastemore » Treatment Plant (LRTP) and the ChNPP Interim Storage Facility for RBMK Fuel Assemblies (ISF). The ENSDF has a capacity of 55,000 m{sup 3}. The primary functions of the ENSDF are: to receive, monitor and record waste packages, to load the waste packages into concrete disposal units, to enable capping and closure of the disposal unit s, to allow monitoring following closure. The ENSDF comprises the turnkey installation of a near surface repository in the form of an engineered facility for the final disposal of LILW-SL conditioned in the ICSRM SWPF and other sources of Chernobyl waste. The project has to deal with the challenges of the Chernobyl environment, the fulfillment of both Western and Ukrainian standards, and the installation and coordination of an international project team. It will be shown that proven technologies and processes can be assembled into a unique Management Concept dealing with all the necessary demands and requirements of a turnkey project. The paper emphasizes the proposed concepts for the ENSDF and their integration into existing infrastructure and installations of the VEKTOR site. Further, the paper will consider the integration of Western and Ukrainian Organizations into a cohesive project team and the requirement to guarantee the fulfillment of both Western standards and Ukrainian regulations and licensing requirements. The paper provides information on the output of the Detail Design and will reflect the progress of the design work.« less

Report of the Seasat Failure Review Board

NASA Technical Reports Server (NTRS)

Lundin, Bruce T.

1978-01-01

The Seasat spacecraft failed on October 9, 1978, after satisfactory operation in orbit for 105 days, as a result of a loss of electrical power in the Agena bus that was used as a part of the spacecraft. This loss of power was caused by a massive and progressive short in one of the slip ring assemblies that was used to connect the rotating solar arrays into the power subsystem. The most likely cause of this short was the initiation of an arc between adjacent slip ring brush assemblies. The triggering mechanism of this arc could have been either a wire-to-brush assembly contact, a brush-to-brush contact, or a momentary short caused by a contaminant that bridged internal components of opposite electrical polarity. The slip ring assembly, as used in the Seasat spacecraft, was connected into the power subsystem in such a way that most of the adjacent brush assemblies were of opposite electrical polarity. This wiring arrangement, together with the congested nature of the design itself, made the Seasat slip ring assembly a unique, first-of-a-kind component that was particularly prone to shorting. The possibility of slip ring failures resulting from placing opposite electrical polarities on adjacent brush assemblies was known at least as early-as the summer of 1977 to other projects within the contractor's organization. Furthermore, failures of slip ring assemblies due to shorting between brushes had been experienced by the prime contractor on slip ring assemblies used by other programs. That the Seasat organization was not fully aware of these potential failure modes was due to a breakdown in communication within the contractor's organization.

Directed assembly of hybrid nanostructures using optically resonant nanotweezers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Erickson, David

This represents the final report for this project. Over the course of the project we have made significant progress in photonically driven nano-assembly including: (1) demonstrating the first direct optical tweezer based manipulation of proteins, (2) the ability to apply optical angular torques to microtubuals and other rod-shaped microparticles, (3) direct assembly of hybrid nanostructures comprising of polymeric nanoparticles and carbon nanotubes and, (4) the ability to drive biological reactions (specifically protein aggregation) that are thermodynamically unfavorable by applying localized optical work. These advancements are described in the list of papers provided in section 2.0 of the below. Summary detailsmore » are provided in prior year annual reports. We have two additional papers which will be submitted shortly based on the work done under this award. An updated publication list will be provided to the program manager when those are accepted. In this report, we report on a new advancement made in the final project year, which uses the nanotweezer technology to perform direct measurements of particle-surface interactions. Briefly, these measurements are important for characterizing the stability and behavior of colloidal and nanoparticle suspensions and current techniques are limited in their ability to measure piconewton scale interaction forces on sub-micrometer particles due to signal detection limits and thermal noise. In this project year we developed a new technique called “Nanophotonic Force Microscopy” which uses the localized region of exponentially decaying, near-field, light to confine small particles close to a surface. From the statistical distribution of the light intensity scattered by the particle the technique maps out the potential well of the trap and directly quantify the repulsive force between the nanoparticle and the surface. The major advantage of the technique is that it can measure forces and energy wells below the thermal noise limit, resolving interaction forces smaller than 1 pN on dielectric particles as small as 100 nm in diameter.« less

Standardized Modular Power Interfaces for Future Space Explorations Missions

NASA Technical Reports Server (NTRS)

Oeftering, Richard

2015-01-01

Earlier studies show that future human explorations missions are composed of multi-vehicle assemblies with interconnected electric power systems. Some vehicles are often intended to serve as flexible multi-purpose or multi-mission platforms. This drives the need for power architectures that can be reconfigured to support this level of flexibility. Power system developmental costs can be reduced, program wide, by utilizing a common set of modular building blocks. Further, there are mission operational and logistics cost benefits of using a common set of modular spares. These benefits are the goals of the Advanced Exploration Systems (AES) Modular Power System (AMPS) project. A common set of modular blocks requires a substantial level of standardization in terms of the Electrical, Data System, and Mechanical interfaces. The AMPS project is developing a set of proposed interface standards that will provide useful guidance for modular hardware developers but not needlessly constrain technology options, or limit future growth in capability. In 2015 the AMPS project focused on standardizing the interfaces between the elements of spacecraft power distribution and energy storage. The development of the modular power standard starts with establishing mission assumptions and ground rules to define design application space. The standards are defined in terms of AMPS objectives including Commonality, Reliability-Availability, Flexibility-Configurability and Supportability-Reusability. The proposed standards are aimed at assembly and sub-assembly level building blocks. AMPS plans to adopt existing standards for spacecraft command and data, software, network interfaces, and electrical power interfaces where applicable. Other standards including structural encapsulation, heat transfer, and fluid transfer, are governed by launch and spacecraft environments and bound by practical limitations of weight and volume. Developing these mechanical interface standards is more difficult but an essential part of defining physical building blocks of modular power. This presentation describes the AMPS projects progress towards standardized modular power interfaces.

Enhancing genome assemblies by integrating non-sequence based data

PubMed Central

2011-01-01

Introduction Many genome projects were underway before the advent of high-throughput sequencing and have thus been supported by a wealth of genome information from other technologies. Such information frequently takes the form of linkage and physical maps, both of which can provide a substantial amount of data useful in de novo sequencing projects. Furthermore, the recent abundance of genome resources enables the use of conserved synteny maps identified in related species to further enhance genome assemblies. Methods The tammar wallaby (Macropus eugenii) is a model marsupial mammal with a low coverage genome. However, we have access to extensive comparative maps containing over 14,000 markers constructed through the physical mapping of conserved loci, chromosome painting and comprehensive linkage maps. Using a custom Bioperl pipeline, information from the maps was aligned to assembled tammar wallaby contigs using BLAT. This data was used to construct pseudo paired-end libraries with intervals ranging from 5-10 MB. We then used Bambus (a program designed to scaffold eukaryotic genomes by ordering and orienting contigs through the use of paired-end data) to scaffold our libraries. To determine how map data compares to sequence based approaches to enhance assemblies, we repeated the experiment using a 0.5× coverage of unique reads from 4 KB and 8 KB Illumina paired-end libraries. Finally, we combined both the sequence and non-sequence-based data to determine how a combined approach could further enhance the quality of the low coverage de novo reconstruction of the tammar wallaby genome. Results Using the map data alone, we were able order 2.2% of the initial contigs into scaffolds, and increase the N50 scaffold size to 39 KB (36 KB in the original assembly). Using only the 0.5× paired-end sequence based data, 53% of the initial contigs were assigned to scaffolds. Combining both data sets resulted in a further 2% increase in the number of initial contigs integrated into a scaffold (55% total) but a 35% increase in N50 scaffold size over the use of sequence-based data alone. Conclusions We provide a relatively simple pipeline utilizing existing bioinformatics tools to integrate map data into a genome assembly which is available at http://www.mcb.uconn.edu/fac.php?name=paska. While the map data only contributed minimally to assigning the initial contigs to scaffolds in the new assembly, it greatly increased the N50 size. This process added structure to our low coverage assembly, greatly increasing its utility in further analyses. PMID:21554765

Enhancing genome assemblies by integrating non-sequence based data.

PubMed

Heider, Thomas N; Lindsay, James; Wang, Chenwei; O'Neill, Rachel J; Pask, Andrew J

2011-05-28

Many genome projects were underway before the advent of high-throughput sequencing and have thus been supported by a wealth of genome information from other technologies. Such information frequently takes the form of linkage and physical maps, both of which can provide a substantial amount of data useful in de novo sequencing projects. Furthermore, the recent abundance of genome resources enables the use of conserved synteny maps identified in related species to further enhance genome assemblies. The tammar wallaby (Macropus eugenii) is a model marsupial mammal with a low coverage genome. However, we have access to extensive comparative maps containing over 14,000 markers constructed through the physical mapping of conserved loci, chromosome painting and comprehensive linkage maps. Using a custom Bioperl pipeline, information from the maps was aligned to assembled tammar wallaby contigs using BLAT. This data was used to construct pseudo paired-end libraries with intervals ranging from 5-10 MB. We then used Bambus (a program designed to scaffold eukaryotic genomes by ordering and orienting contigs through the use of paired-end data) to scaffold our libraries. To determine how map data compares to sequence based approaches to enhance assemblies, we repeated the experiment using a 0.5× coverage of unique reads from 4 KB and 8 KB Illumina paired-end libraries. Finally, we combined both the sequence and non-sequence-based data to determine how a combined approach could further enhance the quality of the low coverage de novo reconstruction of the tammar wallaby genome. Using the map data alone, we were able order 2.2% of the initial contigs into scaffolds, and increase the N50 scaffold size to 39 KB (36 KB in the original assembly). Using only the 0.5× paired-end sequence based data, 53% of the initial contigs were assigned to scaffolds. Combining both data sets resulted in a further 2% increase in the number of initial contigs integrated into a scaffold (55% total) but a 35% increase in N50 scaffold size over the use of sequence-based data alone. We provide a relatively simple pipeline utilizing existing bioinformatics tools to integrate map data into a genome assembly which is available at http://www.mcb.uconn.edu/fac.php?name=paska. While the map data only contributed minimally to assigning the initial contigs to scaffolds in the new assembly, it greatly increased the N50 size. This process added structure to our low coverage assembly, greatly increasing its utility in further analyses.

A system review of the Virginia Alcohol Safety Action Program.

DOT National Transportation Integrated Search

1980-01-01

In March 1979, the Virginia General Assembly gave the Virginia Department of Transportation Safety (VDTS) the power and responsibility to make binding policy and set standards for the local Virginia Alcohol Safety Action Projects across the state. In...

Scheduling "Go with the Flow."

ERIC Educational Resources Information Center

Yucht, Alice

1999-01-01

Presents guidelines for creating a Master Planner for the school year. Highlights several factors to consider, including annual classroom projects; standardized testing dates; report card dates; special assembly programs and local/national/world events; book fairs; and end of the year activities. (AEF)

Evaluation of the Washington state target zero teams project : traffic tech.

DOT National Transportation Integrated Search

2015-01-01

In late 2006, the Washington State Patrol (WSP) assembled : a full-time, high-visibility saturation patrol called the Night : Emphasis Enforcement Team (NEET). This pilot program, : based in Snohomish County and funded by the Washington : Traffic Saf...

Guidance on Preparing a Quality Assurance Project Plan

EPA Pesticide Factsheets

The Guidance is written mainly in a directive format and describes the materials you will need to assemble via lists, questions to think about and/or obtain answers to, and comments or perspective on the contents for each required section.

Light-induced Self-Assembly and Diffusion of Nanoclusters

NASA Astrophysics Data System (ADS)

Lian, Wenxuan

Novel methods to build multiple types of three-dimensional structures from various nanoscale components are the most exciting and challenging questions in nano-science. The properties of the assembled structures can be potentially and designed, but the development of such approaches is challenging. In order to realize such rational assembly, a tunable interaction medium is often introduced into the system. Soft matter, such as polymers, surfactants and biomolecules are used to modify the surfaces of the nanoscale building blocks. Deoxyribonucleic acid (DNA) strands are known as polynucleotides since they are composed of simpler units called nucleotides. There are unique base pairing rules that are predictable and programmable, which can be used to regulate self-assembly process with high degree of control. Besides controlling static structure, it is important to develop methods for controlling systems in dynamic matter, with chemical stimuli or external fields. For example, here we study the use of azobezene-trimethylammonium bromide (AzoTAB) as a molecular agent that can control self-assembly via light excitation. In this thesis, DNA assisted self-assembly was conducted. The ability of AzoTAB as a light induced surfactant to control DNA assisted self-assembly was confirmed. The mechanism of AzoTAB as a light controlled self-assembly promoter was studied. In the second project, diffusion of nanoclusters was studied. The presence of polymers brings strong entanglement with nanoclusters. This entanglement is more obvious when the nanocluster is a framed structure like the octahedron in the study. The diffusion coefficient of the octahedron becomes larger during traveling. The following up studies are required to elucidate the origin of the observed effect.

Method and apparatus for multiple-projection, dual-energy x-ray absorptiometry scanning

NASA Technical Reports Server (NTRS)

Feldmesser, Howard S. (Inventor); Magee, Thomas C. (Inventor); Charles, Jr., Harry K. (Inventor); Beck, Thomas J. (Inventor)

2007-01-01

Methods and apparatuses for advanced, multiple-projection, dual-energy X-ray absorptiometry scanning systems include combinations of a conical collimator; a high-resolution two-dimensional detector; a portable, power-capped, variable-exposure-time power supply; an exposure-time control element; calibration monitoring; a three-dimensional anti-scatter-grid; and a gantry-gantry base assembly that permits up to seven projection angles for overlapping beams. Such systems are capable of high precision bone structure measurements that can support three dimensional bone modeling and derivations of bone strength, risk of injury, and efficacy of countermeasures among other properties.

On initial Brain Activity Mapping of episodic and semantic memory code in the hippocampus.

PubMed

Tsien, Joe Z; Li, Meng; Osan, Remus; Chen, Guifen; Lin, Longian; Wang, Phillip Lei; Frey, Sabine; Frey, Julietta; Zhu, Dajiang; Liu, Tianming; Zhao, Fang; Kuang, Hui

2013-10-01

It has been widely recognized that the understanding of the brain code would require large-scale recording and decoding of brain activity patterns. In 2007 with support from Georgia Research Alliance, we have launched the Brain Decoding Project Initiative with the basic idea which is now similarly advocated by BRAIN project or Brain Activity Map proposal. As the planning of the BRAIN project is currently underway, we share our insights and lessons from our efforts in mapping real-time episodic memory traces in the hippocampus of freely behaving mice. We show that appropriate large-scale statistical methods are essential to decipher and measure real-time memory traces and neural dynamics. We also provide an example of how the carefully designed, sometime thinking-outside-the-box, behavioral paradigms can be highly instrumental to the unraveling of memory-coding cell assembly organizing principle in the hippocampus. Our observations to date have led us to conclude that the specific-to-general categorical and combinatorial feature-coding cell assembly mechanism represents an emergent property for enabling the neural networks to generate and organize not only episodic memory, but also semantic knowledge and imagination. Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

On Initial Brain Activity Mapping of Associative Memory Code in the Hippocampus

PubMed Central

Tsien, Joe Z.; Li, Meng; Osan, Remus; Chen, Guifen; Lin, Longian; Lei Wang, Phillip; Frey, Sabine; Frey, Julietta; Zhu, Dajiang; Liu, Tianming; Zhao, Fang; Kuang, Hui

2013-01-01

It has been widely recognized that the understanding of the brain code would require large-scale recording and decoding of brain activity patterns. In 2007 with support from Georgia Research Alliance, we have launched the Brain Decoding Project Initiative with the basic idea which is now similarly advocated by BRAIN project or Brain Activity Map proposal. As the planning of the BRAIN project is currently underway, we share our insights and lessons from our efforts in mapping real-time episodic memory traces in the hippocampus of freely behaving mice. We show that appropriate large-scale statistical methods are essential to decipher and measure real-time memory traces and neural dynamics. We also provide an example of how the carefully designed, sometime thinking-outside-the-box, behavioral paradigms can be highly instrumental to the unraveling of memory-coding cell assembly organizing principle in the hippocampus. Our observations to date have led us to conclude that the specific-to-general categorical and combinatorial feature-coding cell assembly mechanism represents an emergent property for enabling the neural networks to generate and organize not only episodic memory, but also semantic knowledge and imagination. PMID:23838072

Ergonomic risk assessment with DesignCheck to evaluate assembly work in different phases of the vehicle development process.

PubMed

Winter, Gabriele; Schaub, Karlheinz G; Großmann, Kay; Laun, Gerhard; Landau, Kurt; Bruder, Ralph

2012-01-01

Occupational hazards exist, if the design of the work situation is not in accordance with ergonomic design principles. At assembly lines ergonomics is applied to the design of work equipment and tasks and to work organisation. The ignoring of ergonomic principles in planning and design of assembly work leads to unfavourable working posture, action force and material handling. Disorders of the musculoskeletal system are of a common occurrence throughout Europe. Musculoskeletal disorders are a challenge against the background of disabled workers. The changes in a worker's capability have to be regarded in the conception of redesigned and new assembly lines. In this way ergonomics becomes progressively more important in planning and design of vehicles: The objective of ergonomic design in different stages of the vehicles development process is to achieve an optimal adaptation of the assembly work to workers. Hence the ergonomic screening tool "Design Check" (DC) was developed to identify ergonomic deficits in workplace layouts. The screening-tool is based on the current ergonomic state of the art in the design of physical work and relevant EU legal requirements. It was tested within a federal German research project at selected work stations at the assembly lines at Dr.-Ing. h.c. F. Porsche AG / Stuttgart. Meanwhile the application of the screening-tool DC is transferred in other parts of the Porsche AG, Stuttgart. It is also realized as an ergonomic standard method to perform assembly work in different phases of the vehicle development process.

Outcomes of the JNT 1955 Phase I Viability Study of Gamma Emission Tomography for Spent Fuel Verification

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jacobsson-Svard, Staffan; Smith, Leon E.; White, Timothy

The potential for gamma emission tomography (GET) to detect partial defects within a spent nuclear fuel assembly has been assessed within the IAEA Support Program project JNT 1955, phase I, which was completed and reported to the IAEA in October 2016. Two safeguards verification objectives were identified in the project; (1) independent determination of the number of active pins that are present in a measured assembly, in the absence of a priori information about the assembly; and (2) quantitative assessment of pin-by-pin properties, for example the activity of key isotopes or pin attributes such as cooling time and relative burnup,more » under the assumption that basic fuel parameters (e.g., assembly type and nominal fuel composition) are known. The efficacy of GET to meet these two verification objectives was evaluated across a range of fuel types, burnups and cooling times, while targeting a total interrogation time of less than 60 minutes. The evaluations were founded on a modelling and analysis framework applied to existing and emerging GET instrument designs. Monte Carlo models of different fuel types were used to produce simulated tomographer responses to large populations of “virtual” fuel assemblies. The simulated instrument response data were then processed using a variety of tomographic-reconstruction and image-processing methods, and scoring metrics were defined and used to evaluate the performance of the methods.This paper describes the analysis framework and metrics used to predict tomographer performance. It also presents the design of a “universal” GET (UGET) instrument intended to support the full range of verification scenarios envisioned by the IAEA. Finally, it gives examples of the expected partial-defect detection capabilities for some fuels and diversion scenarios, and it provides a comparison of predicted performance for the notional UGET design and an optimized variant of an existing IAEA instrument.« less

Potential Natural Vegetation of the Mississippi Alluvial Valley: Boeuf-Tensas Basin, Arkansas, Field Atlas

DTIC Science & Technology

2012-09-01

under the auspices of federal and state research programs or in conjunction with Corps of Engineers project planning efforts. In the process , a...in the field effort and assembled and processed the original project GIS data. Malcolm Williamson (Center for Advanced Spatial Technologies...further improve drainage. ERDC/EL TR-12-28 5 3 Using the PNV map as a model for restoration The PNV mapping process was conceived as a way to

Advanced Manufacturing Technologies (AMT): Additive Manufactured Hot Fire Planning and Testing in GRC Cell 32 Project

NASA Technical Reports Server (NTRS)

Fikes, John C.

2014-01-01

The objective of this project is to hot fire test an additively manufactured thrust chamber assembly TCA (injector and thrust chamber). GRC will install the additively manufactured Inconel 625 injector, two additively manufactured (SLM) water cooled Cu-Cr thrust chamber barrels and one additively manufactured (SLM) water cooled Cu-Cr thrust chamber nozzle on the test stand in Cell 32 and perform hot fire testing of the integrated TCA.

CO2 Compressor Requirements for Integration of Space Station Carbon Dioxide Removal and Carbon Dioxide Reduction Assemblies

NASA Technical Reports Server (NTRS)

Jeng, Frank F.; Lewis, John F.; Graf, John; LaFuse, Sharon; Nicholson, Leonard S. (Technical Monitor)

1999-01-01

This paper describes the analysis on integration requirements, CO2 compressor in particular, for integration of Carbon Dioxide Removal Assembly (CDRA) and CO2 Reduction Assembly (CRA) as a part of the Node 3 project previously conducted at JSC/NASA. A system analysis on the volume and operation pressure range of the CO2 accumulator was conducted. The hardware and operational configurations of the CO2 compressor were developed. The performance and interface requirements of the compressor were specified. An existing Four-Bed Molecular Sieve CO2 removal computer model was modified into a CDRA model and used in analyzing the requirements of the CDRA CO2 compressor. This CDRA model was also used in analyzing CDRA operation parameters that dictate CO2 pump sizing. Strategy for the pump activation was also analyzed.

Sandia National Laboratories, Tonopah Test Range Assembly Building 9B (Building 09-54): Photographs and Written Historical and Descriptive Data

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ullrich, Rebecca A.

Assembly Building 9B (Building 09-54) is a contributing element to the Sandia National Laboratories (SNL) Tonopah Test Range (TTR) Historic District. The SNL TTR Historic District played a significant role in U.S. Cold War history in the areas of stockpile surveillance and non-nuclear field testing of nuclear weapons designs. The district covers approximately 179,200 acres and illustrates Cold War development testing of nuclear weapons components and systems. This report includes historical information, architectural information, sources of information, project information, maps, blueprints, and photographs.

Comparison of the Booster Interface Temperature in Stainless Steel (SS) V-Channel Versus the Aluminum (Al) Y-Channel Primer Chamber Assemblies (PCAs). Volume 2; Appendices

NASA Technical Reports Server (NTRS)

Garcia, Roberto; Saulsberry, Regor L.

2011-01-01

NASA's Technical Fellow for Propulsion, requested a technical assessment of the performance improvement achieved by the introduction of the stainless steel (SS) V-channel compared to the aluminum (Al) Y-channel Primer Chamber Assembly (PCA) design. The SS V-channel PCA was developed for NASA's Mars Science Laboratory (MSL) Project. The principle focus of the assessment was to measure the transient temperature at the booster interface with both designs. This document contains the Appendices to the Volume I main report.

STRUCTURE FOR SUB-ASSEMBLIES OF ELECTRONIC EQUIPMENT

DOEpatents

Bell, P.R.; Harris, C.C.

1959-03-31

Sub-assemblies for electronic systems, particularly a unit which is self- contained and which may be adapted for quick application to and detachment from a chassis or panel, are discussed. The disclosed structure serves the dual purpose of a cover or enclosure for a subassembly comprising a base plate and also acts as a clamp for retaining the base plate in position on a chassis. The clamping action is provided by flexible fingers projecting from the side walls of the cover and extending through grooves in the base plate to engage with the opposite side of the chassis.

Comparison of the Booster Interface Temperature in Stainless Steel (SS) V-Channel versus the Aluminum (Al) Y-Channel Primer Chamber Assemblies (PCAs). Volume 1; Technical Assessment Report

NASA Technical Reports Server (NTRS)

Garcia, Roberto; Saulsberry, Regor L.

2011-01-01

NASA's Technical Fellow for Propulsion, requested a technical assessment of the performance improvement achieved by the introduction of the stainless steel (SS) V-channel compared to the aluminum (Al) Y-channel Primer Chamber Assembly (PCA) design. The SS V-channel PCA was developed for NASA's Mars Science Laboratory (MSL) Project. The principle focus of the assessment was to measure the transient temperature at the booster interface with both designs. This document contains the findings of the assessment.

SYNCSA--R tool for analysis of metacommunities based on functional traits and phylogeny of the community components.

PubMed

Debastiani, Vanderlei J; Pillar, Valério D

2012-08-01

SYNCSA is an R package for the analysis of metacommunities based on functional traits and phylogeny of the community components. It offers tools to calculate several matrix correlations that express trait-convergence assembly patterns, trait-divergence assembly patterns and phylogenetic signal in functional traits at the species pool level and at the metacommunity level. SYNCSA is a package for the R environment, under a GPL-2 open-source license and freely available on CRAN official web server for R (http://cran.r-project.org). vanderleidebastiani@yahoo.com.br.

The MINE project: Minority Involvement in NASA Engineering

NASA Technical Reports Server (NTRS)

Allen, H., Jr.

1977-01-01

The Mine Project developed by Lewis Research Center (LRC) along with Tennessee State University and Tuskegee Institute, is described. The project calls for LRC to assemble on-going NASA university affairs programs aimed at benefiting the school, its faculty, and its student body. The schools receive grants to pursue research and technology projects that are relevant to NASA's missions. Upon request from the universities, LRC furnishes instructors and lecturers. The schools have use of surplus government equipment and access to NASA research facilities for certain projects. Both the faculty and students of the universities are eligible for summer employment at LRC through special programs. The MINE Project is designed to establish a continuing active relationship of 3 to 5 years between NASA and the universities, and will afford LRC with an opportunity to increase its recruitment of minority and women employees.

Citizen Sky, An Update on the AAVSO's New Citizen Science Project

NASA Astrophysics Data System (ADS)

Turner, Rebecca; Price, A.; Henden, A.; Stencel, R.; Kloppenborg, B.

2011-01-01

Citizen Sky is a multi-year, NSF-funded, citizen science project focusing on the bright variable star, epsilon Aurigae. Citizen Sky goes beyond simple observing to include a major data analysis component. The goal is to introduce the participant to the full scientific process from background research to paper writing for a peer-reviewed journal. The first year of the project, 2009-10, was dedicated to developing project infrastructure, educating participants about epsilon Aurigae, and training these participants to observe the star and report their data. Looking forward, years two and three of the project will focus on assembling teams of participants to work on their own analysis and research. Results will be published in a special issue of the peer-reviewed Journal of the AAVSO. This project has been made possible by the National Science Foundation.

Sequencing three crocodilian genomes to illuminate the evolution of archosaurs and amniotes

PubMed Central

2012-01-01

The International Crocodilian Genomes Working Group (ICGWG) will sequence and assemble the American alligator (Alligator mississippiensis), saltwater crocodile (Crocodylus porosus) and Indian gharial (Gavialis gangeticus) genomes. The status of these projects and our planned analyses are described. PMID:22293439

A system review of the Virginia Alcohol Safety Action Program : executive summary.

DOT National Transportation Integrated Search

1980-01-01

In March 1979, the Virginia General Assembly gave the Virginia Department of Transportation Safety (VDTS) the power and responsibility to make binding policy and set standards for the local Virginia Alcohol Safety Action Projects across the state. In...

Implications of the Tribolium genome project for pest biology

USDA-ARS?s Scientific Manuscript database

The universal availability of the complete Tribolium castaneum genome sequence assembly and annotation and concomitant development of the versatile Tribolium genome browser, BeetleBase (http://beetlebase.org/) open new realms of possibility for stored-product pest control by greatly simplifying the...

Indicators of Future School Enrollments.

ERIC Educational Resources Information Center

Educational Research Service, Arlington, VA.

Data collected by United States government agencies, plus private health and demographic organizations, are assembled to provide reliable information regarding trends and projections of births, migration, and other indicators of school enrollment. The report, intended for educational planners, provides an overview of future school enrollment…

An empirical model for optimal highway durability in cold regions.

DOT National Transportation Integrated Search

2016-03-10

We develop an empirical tool to estimate optimal highway durability in cold regions. To test the model, we assemble a data set : containing all highway construction and maintenance projects in Arizona and Washington State from 1990 to 2014. The data ...

The on-board tailpipe emissions measurement system (TOTEMS) : proof\\0x2010 of\\0x2010concept.

DOT National Transportation Integrated Search

2009-06-03

An on-board tailpipe emissions instrumentation system was designed, assembled and tested as proof-of-concept : for the University of Vermonts Transportation Research Center (TRC) Signature Project #2 real-world vehicle : emissions data colle...

Thermal cycling test results of CSP and RF assemblies

NASA Technical Reports Server (NTRS)

Ghaffarian, R.; Nelson, G.; Cooper, M.; Lam, D.; Strudler, S.; Umdekar, A.; Selk, K.; Bjorndahl, B.; Duprey, R.

2000-01-01

A JPL-led chip scale package (CSP) Consortium of enterprises, composed of representing agencies and private companies, recently joined together to pool in-kind resources for developing the quality and reliability of chip scale packages (CSPs) for a variety of projects.

MaizeGDB, the maize model organism database

USDA-ARS?s Scientific Manuscript database

MaizeGDB is the maize research community's database for maize genetic and genomic information. In this seminar I will outline our current endeavors including a full website redesign, the status of maize genome assembly and annotation projects, and work toward genome functional annotation. Mechanis...

The World Assembly on Aging--Three Years Later.

ERIC Educational Resources Information Center

Nusberg, Charlotte

1985-01-01

Examines the world response to population aging. Discusses national, regional, and international activities, such as conferences, campaigns to promote an active aging, employment projects, and community alternatives to institutional care. Policy responses are considered, involving employment, income, health, housing, family, consumerism,…

42 CFR 67.21 - Control of data and availability of publications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Grants for Health Services Research, Evaluation, Demonstration, and Dissemination Projects § 67.21... assembled for the purpose of carrying out health services research, evaluation, demonstration, or... possible to a variety of audiences, including health care providers, practitioners, consumers, educators...

The Big Brown Bus: Teaching Future Elementary School Teachers about Mass Production.

ERIC Educational Resources Information Center

Linnell, Charles C.

1996-01-01

Describes a project that helps teachers introduce the concept of mass production in the elementary classroom. Provides instructions for the activity in which students produce "buses" made of juice containers and brown paper on an assembly line. (JOW)

Neutron Based Non-Destructive Assay (NDA) Measurement Systems for Safeguard

DOE Office of Scientific and Technical Information (OSTI.GOV)

Swinhoe, Martyn Thomas

2017-09-21

The objectives of this project are to introduce the assay methods for plutonium measurements using the HLNC; introduce the assay method for bulk uranium measurements using the AWCC; and introduce the assay method for fuel assembly measurements using the UNCL.

Looking Across Many Cancer Genomes - TCGA

Cancer.gov

TCGA researchers have developed a formal project for a cross tumor analysis, called Pan-Cancer. Its goal is to assemble TCGA’s wealth of data across tumor types, analyze and interpret those data, and finally, make both the analyses and the data freely available.

Knowledge-based decision support for Space Station assembly sequence planning

NASA Astrophysics Data System (ADS)

1991-04-01

A complete Personal Analysis Assistant (PAA) for Space Station Freedom (SSF) assembly sequence planning consists of three software components: the system infrastructure, intra-flight value added, and inter-flight value added. The system infrastructure is the substrate on which software elements providing inter-flight and intra-flight value-added functionality are built. It provides the capability for building representations of assembly sequence plans and specification of constraints and analysis options. Intra-flight value-added provides functionality that will, given the manifest for each flight, define cargo elements, place them in the National Space Transportation System (NSTS) cargo bay, compute performance measure values, and identify violated constraints. Inter-flight value-added provides functionality that will, given major milestone dates and capability requirements, determine the number and dates of required flights and develop a manifest for each flight. The current project is Phase 1 of a projected two phase program and delivers the system infrastructure. Intra- and inter-flight value-added were to be developed in Phase 2, which has not been funded. Based on experience derived from hundreds of projects conducted over the past seven years, ISX developed an Intelligent Systems Engineering (ISE) methodology that combines the methods of systems engineering and knowledge engineering to meet the special systems development requirements posed by intelligent systems, systems that blend artificial intelligence and other advanced technologies with more conventional computing technologies. The ISE methodology defines a phased program process that begins with an application assessment designed to provide a preliminary determination of the relative technical risks and payoffs associated with a potential application, and then moves through requirements analysis, system design, and development.

Knowledge-based decision support for Space Station assembly sequence planning

NASA Technical Reports Server (NTRS)

1991-01-01

A complete Personal Analysis Assistant (PAA) for Space Station Freedom (SSF) assembly sequence planning consists of three software components: the system infrastructure, intra-flight value added, and inter-flight value added. The system infrastructure is the substrate on which software elements providing inter-flight and intra-flight value-added functionality are built. It provides the capability for building representations of assembly sequence plans and specification of constraints and analysis options. Intra-flight value-added provides functionality that will, given the manifest for each flight, define cargo elements, place them in the National Space Transportation System (NSTS) cargo bay, compute performance measure values, and identify violated constraints. Inter-flight value-added provides functionality that will, given major milestone dates and capability requirements, determine the number and dates of required flights and develop a manifest for each flight. The current project is Phase 1 of a projected two phase program and delivers the system infrastructure. Intra- and inter-flight value-added were to be developed in Phase 2, which has not been funded. Based on experience derived from hundreds of projects conducted over the past seven years, ISX developed an Intelligent Systems Engineering (ISE) methodology that combines the methods of systems engineering and knowledge engineering to meet the special systems development requirements posed by intelligent systems, systems that blend artificial intelligence and other advanced technologies with more conventional computing technologies. The ISE methodology defines a phased program process that begins with an application assessment designed to provide a preliminary determination of the relative technical risks and payoffs associated with a potential application, and then moves through requirements analysis, system design, and development.

Assembly of 500,000 inter-specific catfish expressed sequence tags and large scale gene-associated marker development for whole genome association studies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Catfish Genome Consortium; Wang, Shaolin; Peatman, Eric

2010-03-23

Background-Through the Community Sequencing Program, a catfish EST sequencing project was carried out through a collaboration between the catfish research community and the Department of Energy's Joint Genome Institute. Prior to this project, only a limited EST resource from catfish was available for the purpose of SNP identification. Results-A total of 438,321 quality ESTs were generated from 8 channel catfish (Ictalurus punctatus) and 4 blue catfish (Ictalurus furcatus) libraries, bringing the number of catfish ESTs to nearly 500,000. Assembly of all catfish ESTs resulted in 45,306 contigs and 66,272 singletons. Over 35percent of the unique sequences had significant similarities tomore » known genes, allowing the identification of 14,776 unique genes in catfish. Over 300,000 putative SNPs have been identified, of which approximately 48,000 are high-quality SNPs identified from contigs with at least four sequences and the minor allele presence of at least two sequences in the contig. The EST resource should be valuable for identification of microsatellites, genome annotation, large-scale expression analysis, and comparative genome analysis. Conclusions-This project generated a large EST resource for catfish that captured the majority of the catfish transcriptome. The parallel analysis of ESTs from two closely related Ictalurid catfishes should also provide powerful means for the evaluation of ancient and recent gene duplications, and for the development of high-density microarrays in catfish. The inter- and intra-specific SNPs identified from all catfish EST dataset assembly will greatly benefit the catfish introgression breeding program and whole genome association studies.« less

Modeling Effects of RNA on Capsid Assembly Pathways via Coarse-Grained Stochastic Simulation

PubMed Central

Smith, Gregory R.; Xie, Lu; Schwartz, Russell

2016-01-01

The environment of a living cell is vastly different from that of an in vitro reaction system, an issue that presents great challenges to the use of in vitro models, or computer simulations based on them, for understanding biochemistry in vivo. Virus capsids make an excellent model system for such questions because they typically have few distinct components, making them amenable to in vitro and modeling studies, yet their assembly can involve complex networks of possible reactions that cannot be resolved in detail by any current experimental technology. We previously fit kinetic simulation parameters to bulk in vitro assembly data to yield a close match between simulated and real data, and then used the simulations to study features of assembly that cannot be monitored experimentally. The present work seeks to project how assembly in these simulations fit to in vitro data would be altered by computationally adding features of the cellular environment to the system, specifically the presence of nucleic acid about which many capsids assemble. The major challenge of such work is computational: simulating fine-scale assembly pathways on the scale and in the parameter domains of real viruses is far too computationally costly to allow for explicit models of nucleic acid interaction. We bypass that limitation by applying analytical models of nucleic acid effects to adjust kinetic rate parameters learned from in vitro data to see how these adjustments, singly or in combination, might affect fine-scale assembly progress. The resulting simulations exhibit surprising behavioral complexity, with distinct effects often acting synergistically to drive efficient assembly and alter pathways relative to the in vitro model. The work demonstrates how computer simulations can help us understand how assembly might differ between the in vitro and in vivo environments and what features of the cellular environment account for these differences. PMID:27244559

Patchwork Plagiarism

ERIC Educational Resources Information Center

Cooper, Janice

2007-01-01

In this Information Age, students produce research projects by piecing together passages quoted verbatim and stitched together with a few introductory or transitional words. They assemble research as patchwork quilts rather than weaving a fabric of new knowledge. This common practice raises ethical issues and poses new challenges for library media…

DOE Office of Scientific and Technical Information (OSTI.GOV)

Joshi, Jay Prakash

The objectives of this project are to calibrate the Advanced Experimental Fuel Counter (AEFC), benchmark MCNP simulations using experimental results, investigate the effects of change in fuel assembly geometry, and finally to show the boost in doubles count rates with 252Cf active soruces due to the time correlated induced fission (TCIF) effect.

Words in Action: Rethinking Workplace Literacy.

ERIC Educational Resources Information Center

Rose, Mike

2003-01-01

Discusses a research project exploring the thought it takes to do physical work, the cognitive processes involved in various blue collar and service occupations like waitressing, hairstyling, plumbing, welding, industrial assembly, and the like. Considers the way occupations are categorized, defines intelligence, and thinks about learning and…

CPAS Parachute Testing, Model Development, & Verification

NASA Technical Reports Server (NTRS)

Romero, Leah M.

2013-01-01

Capsule Parachute Assembly System (CPAS) is the human rated parachute system for the Orion vehicle used during re-entry. Similar to Apollo parachute design. Human rating requires additional system redundancy. A Government Furnished Equipment (GFE) project responsible for: Design; Development testing; Performance modeling; Fabrication; Qualification; Delivery

Internet Implementation in Brazilian K-12 Schools.

ERIC Educational Resources Information Center

Joia, Luiz Antonio

1997-01-01

Presents a framework for implementing the Internet in Brazilian elementary and secondary schools. Topics include schools as assembly lines in the information society; pedagogical uses of the Internet, including research, educational projects, and video conferences; and obstacles to implementing the Internet, including cost, cultural differences,…

Magnetic Measurements of Storage Ring Magnets for the APS Upgrade Project

DOE Office of Scientific and Technical Information (OSTI.GOV)

Doose, C.; Dejus, R.; Jaski, M.

2017-06-01

Extensive prototyping of storage ring magnets is ongoing at the Advanced Photon Source (APS) in support of the APS Multi-Bend Achromat (MBA) upgrade project (APS-U) [1]. As part of the R&D activities four quadrupole magnets with slightly different geometries and pole tip materials, and one sextupole magnet with vanadium permendur (VP) pole tips were designed, built and tested. Magnets were measured individually using a rotating coil and a Hall probe for detailed mapping of the magnetic field. Magnets were then assembled and aligned relative to each other on a steel support plate and concrete plinth using precision machined surfaces tomore » gain experience with the alignment method chosen for the APS-U storage ring magnets. The required alignment of magnets on a common support structure is 30 μm rms. Measurements of magnetic field quality, strength and magnet alignment after subjecting the magnets and assemblies to different tests are presented.« less

Research and Development of External Occultor Technology for the Direct Observation of Extrasolar Planetary Systems : JPL Starshades Project

NASA Technical Reports Server (NTRS)

Franz, Herbert; Stadeler, Mehnert

2012-01-01

Our group conducted work during the Summer of 2012 assembling and developing JPL's Starshades Project under the Technology Development for Exoplanet Missions(TDEM) initiative created by NASA, specifically TDEM stage 2. The goal of the work conducted at JPL by our group was to construct four occultor petals, the main optical components of the Starshade, for the analysis of joint deployment characteristics and of mechanical strain. A Starshade is an optical structure measuring approximately 30 meters in diameter that uses the effects of light diffraction off sheer edges, light scattering, and negative interference between waves to negate all on-axis light in a telescope's image, providing very high contrast that allows planets orbiting a target star to be observed. We completed our engineering goals in the time span of 10 weeks, during which the assembly processes of manufacture, alignment, and structural bonding took place. The Starshade technology and construction process is further discussed in the body of this paper.

The development of alignment turning system for precision len cells

NASA Astrophysics Data System (ADS)

Huang, Chien-Yao; Ho, Cheng-Fang; Wang, Jung-Hsing; Chung, Chien-Kai; Chen, Jun-Cheng; Chang, Keng-Shou; Kuo, Ching-Hsiang; Hsu, Wei-Yao; Chen, Fong-Zhi

2017-08-01

In general, the drop-in and cell-mounted assembly are used for standard and high performance optical system respectively. The optical performance is limited by the residual centration error and position accuracy of the conventional assembly. Recently, the poker chip assembly with high precision lens barrels that can overcome the limitation of conventional assembly is widely applied to ultra-high performance optical system. ITRC also develops the poker chip assembly solution for high numerical aperture objective lenses and lithography projection lenses. In order to achieve high precision lens cell for poker chip assembly, an alignment turning system (ATS) is developed. The ATS includes measurement, alignment and turning modules. The measurement module including a non-contact displacement sensor and an autocollimator can measure centration errors of the top and the bottom surface of a lens respectively. The alignment module comprising tilt and translation stages can align the optical axis of the lens to the rotating axis of the vertical lathe. The key specifications of the ATS are maximum lens diameter, 400mm, and radial and axial runout of the rotary table < 2 μm. The cutting performances of the ATS are surface roughness Ra < 1 μm, flatness < 2 μm, and parallelism < 5 μm. After measurement, alignment and turning processes on our ATS, the centration error of a lens cell with 200mm in diameter can be controlled in 10 arcsec. This paper also presents the thermal expansion of the hydrostatic rotating table. A poker chip assembly lens cell with three sub-cells is accomplished with average transmission centration error in 12.45 arcsec by fresh technicians. The results show that ATS can achieve high assembly efficiency for precision optical systems.

The high energy astronomy observatories

NASA Technical Reports Server (NTRS)

Neighbors, A. K.; Doolittle, R. F.; Halpers, R. E.

1977-01-01

The forthcoming NASA project of orbiting High Energy Astronomy Observatories (HEAO's) designed to probe the universe by tracing celestial radiations and particles is outlined. Solutions to engineering problems concerning HEAO's which are integrated, yet built to function independently are discussed, including the onboard digital processor, mirror assembly and the thermal shield. The principle of maximal efficiency with minimal cost and the potential capability of the project to provide explanations to black holes, pulsars and gamma-ray bursts are also stressed. The first satellite is scheduled for launch in April 1977.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Benedek, George; Casparay, Alfred H.

In this project, we are developing a new system for measuring forces within and between nanoscale biological molecules based on mesoscopic springs made of cholesterol helical ribbons. These ribbons self-assemble in a wide variety of complex fluids containing sterol, a mixture of surfactants and water [1] and have spring constants in the range from 0.5 to 500 pN/nm [2-4]. By the end of this project, we have demonstrated that the cholesterol helical ribbons can be used for measuring forces between biological objects and for mapping the strain fields in hydrogels.

Modeling of Revitalization of Atmospheric Water

NASA Technical Reports Server (NTRS)

Coker, Robert; Knox, Jim

2014-01-01

The Atmosphere Revitalization Recovery and Environmental Monitoring (ARREM) project was initiated in September of 2011 as part of the Advanced Exploration Systems (AES) program. Under the ARREM project, testing of sub-scale and full-scale systems has been combined with multiphysics computer simulations for evaluation and optimization of subsystem approaches. In particular, this paper describes the testing and modeling of the water desiccant subsystem of the carbon dioxide removal assembly (CDRA). The goal is a full system predictive model of CDRA to guide system optimization and development.

Neutral Buoyancy Simulator-NB32-Assembly of Large Space Structure

NASA Technical Reports Server (NTRS)

1980-01-01

Once the United States' space program had progressed from Earth's orbit into outerspace, theprospect of building and maintaining a permanent presence in space was realized. To accomplish this feat, NASA launched a temporary workstation, Skylab, to discover the effects of low gravity and weightlessness on the human body, and also to develop tools and equipment that would be needed in the future to build and maintain a more permanent space station. The structures, techniques, and work schedules had to be carefully designed to fit this unique construction site. The components had to be lightweight for transport into orbit, yet durable. The station also had to be made with removable parts for easy servicing and repairs by astronauts. All of the tools necessary for service and repairs had to be designed for easy manipulation by a suited astronaut. Construction methods had to be efficient due to the limited time the astronauts could remain outside their controlled environment. In lieu of all the specific needs for this project, an environment on Earth had to be developed that could simulate a low gravity atmosphere. A Neutral Buoyancy Simulator (NBS) was constructed by NASA's Marshall Space Flight Center (MSFC) in 1968. Since then, NASA scientists have used this facility to understand how humans work best in low gravity and also provide information about the different kinds of structures that can be built. Pictured is a Massachusetts Institute of Technology (MIT) student working in a spacesuit on the Experimental Assembly of Structures in Extravehicular Activity (EASE) project which was developed as a joint effort between MFSC and MIT. The EASE experiment required that crew members assemble small components to form larger components, working from the payload bay of the space shuttle. The MIT student in this photo is assembling two six-beam tetrahedrons.

Space Shuttle Projects

NASA Image and Video Library

1985-11-30

The crew assigned to the STS-61B mission included Bryan D. O’Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission’s primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia and the Marshall Space Flight Center (MSFC), EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). The primary objective of this experiment was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction. In this STS-61B onboard photo, astronaut Ross was working on the ACCESS experiment during an Extravehicular Activity (EVA).

Space Shuttle Projects

NASA Image and Video Library

1985-11-30

The crew assigned to the STS-61B mission included Bryan D. O’Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission’s primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia, and the Marshall Space Flight Center (MSFC), EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). In this STS-61B onboard photo, astronaut Ross works on ACCESS high above the orbiter. The primary objective of these experiments was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

Critical assembly: A technical history of Los Alamos during the Oppenheimer years, 1943--1945

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hoddeson, L.; Henriksen, P.W.; Meade, R.A.

1993-11-01

This volume treats the technical research that led to the first atomic bombs. The authors explore how the ``critical assembly`` of scientists, engineers, and military Personnel at Los Alamos collaborated during World War II, blending their traditions to create a new approach to large-scale research. The research was characterized by strong mission orientation, multidisciplinary teamwork, expansion of the scientists` traditional methodology with engineering techniques, and a trail-and-error methodology responding to wartime deadlines. The book opens with an introduction laying out major themes. After a synopsis of the prehistory of the bomb project, from the discovery of nuclear fission to themore » start of the Manhattan Engineer District, and an overview of the early materials program, the book examines the establishment of the Los Alamos Laboratory, the implosion and gun assembly programs, nuclear physics research, chemistry and metallurgy, explosives, uranium and plutonium development, confirmation of spontaneous fission in pile-produced plutonium, the thermonuclear bomb, critical assemblies, the Trinity test, and delivery of the combat weapons.« less

Space Shuttle Projects

NASA Image and Video Library

1985-11-30

The crew assigned to the STS-61B mission included Bryan D. O’Conner, pilot; Brewster H. Shaw, commander; Charles D. Walker, payload specialist; mission specialists Jerry L. Ross, Mary L. Cleave, and Sherwood C. Spring; and Rodolpho Neri Vela, payload specialist. Launched aboard the Space Shuttle Atlantis November 28, 1985 at 7:29:00 pm (EST), the STS-61B mission’s primary payload included three communications satellites: MORELOS-B (Mexico); AUSSAT-2 (Australia); and SATCOM KU-2 (RCA Americom). Two experiments were conducted to test assembling erectable structures in space: EASE (Experimental Assembly of Structures in Extravehicular Activity), and ACCESS (Assembly Concept for Construction of Erectable Space Structure). In a joint venture between NASA/Langley Research Center in Hampton, Virginia, and the Marshall Space Flight Center (MSFC), the EASE and ACCESS were developed and demonstrated at MSFC's Neutral Buoyancy Simulator (NBS). In this STS-61B onboard photo, astronaut Spring was working on the EASE during an Extravehicular Activity (EVA). The primary objective of this experiment was to test the structural assembly concepts for suitability as the framework for larger space structures and to identify ways to improve the productivity of space construction.

Automated Array Assembly, Phase 2. Low-cost Solar Array Project, Task 4

NASA Technical Reports Server (NTRS)

Lopez, M.

1978-01-01

Work was done to verify the technological readiness of a select process sequence with respect to satisfying the Low Cost Solar Array Project objectives of meeting the designated goals of $.50 per peak watt in 1986 (1975 dollars). The sequence examined consisted of: (1) 3 inches diameter as-sawn Czochralski grown 1:0:0 silicon, (2) texture etching, (3) ion implanting, (4) laser annealing, (5) screen printing of ohmic contacts and (6) sprayed anti-reflective coatings. High volume production projections were made on the selected process sequence. Automated processing and movement of hardware at high rates were conceptualized to satisfy the PROJECT's 500 MW/yr capability. A production plan was formulated with flow diagrams integrating the various processes in the cell fabrication sequence.

Fabrication of Nonperiodic Metasurfaces by Microlens Projection Lithography.

PubMed

Gonidec, Mathieu; Hamedi, Mahiar M; Nemiroski, Alex; Rubio, Luis M; Torres, Cesar; Whitesides, George M

2016-07-13

This paper describes a strategy that uses template-directed self-assembly of micrometer-scale microspheres to fabricate arrays of microlenses for projection photolithography of periodic, quasiperiodic, and aperiodic infrared metasurfaces. This method of "template-encoded microlens projection lithography" (TEMPL) enables rapid prototyping of planar, multiscale patterns of similarly shaped structures with critical dimensions down to ∼400 nm. Each of these structures is defined by local projection lithography with a single microsphere acting as a lens. This paper explores the use of TEMPL for the fabrication of a broad range of two-dimensional lattices with varying types of nonperiodic spatial distribution. The matching optical spectra of the fabricated and simulated metasurfaces confirm that TEMPL can produce structures that conform to expected optical behavior.

Computer Simulation and Modeling of CO2 Removal Systems for Exploration 2013-2014

NASA Technical Reports Server (NTRS)

Coker, R.; Knox, J.; Gomez, C.

2015-01-01

The Atmosphere Revitalization Recovery and Environmental Monitoring (ARREM) project was initiated in September of 2011 as part of the Advanced Exploration Systems (AES) program. Under the ARREM project and the follow-on Life Support Systems (LSS) project, testing of sub-scale and full-scale systems has been combined with multiphysics computer simulations for evaluation and optimization of subsystem approaches. In particular, this paper will describes the testing and 1-D modeling of the combined water desiccant and carbon dioxide sorbent subsystems of the carbon dioxide removal assembly (CDRA). The goal is a full system predictive model of CDRA to guide system optimization and development.

Final Scientific/Technical Report -- Single-Junction Organic Solar Cells with >15% Efficiency

DOE Office of Scientific and Technical Information (OSTI.GOV)

Starkenburg, Daken; Weldeab, Asmerom; Fagnani, Dan

Organic solar cells have the potential to offer low-cost solar energy conversion due to low material costs and compatibility with low-temperature and high throughput manufacturing processes. This project aims to further improve the efficiency of organic solar cells by applying a previously demonstrated molecular self-assembly approach to longer-wavelength light-absorbing organic materials. The team at the University of Florida designed and synthesized a series of low-bandgap organic semiconductors with functional hydrogen-bonding groups, studied their assembly characteristics and optoelectronic properties in solid-state thin film, and fabricated organic solar cells using solution processing. These new organic materials absorb light up 800 nm wavelength,more » and provide a maximum open-circuit voltage of 1.05 V in the resulted solar cells. The results further confirmed the effectiveness in this approach to guide the assembly of organic semiconductors in thin films to yield higher photovoltaic performance for solar energy conversion. Through this project, we have gained important understanding on designing, synthesizing, and processing organic semiconductors that contain appropriately functionalized groups to control the morphology of the organic photoactive layer in solar cells. Such fundamental knowledge could be used to further develop new functional organic materials to achieve higher photovoltaic performance, and contribute to the eventual commercialization of the organic solar cell technology.« less

Fresh Fuel Measurements With the Differential Die-Away Self-Interrogation Instrument

NASA Astrophysics Data System (ADS)

Trahan, Alexis C.; Belian, Anthony P.; Swinhoe, Martyn T.; Menlove, Howard O.; Flaska, Marek; Pozzi, Sara A.

2017-07-01

The purpose of the Next Generation Safeguards Initiative (NGSI)-Spent Fuel (SF) Project is to strengthen the technical toolkit of safeguards inspectors and/or other interested parties. The NGSI-SF team is working to achieve the following technical goals more easily and efficiently than in the past using nondestructive assay measurements of spent fuel assemblies: 1) verify the initial enrichment, burnup, and cooling time of facility declaration; 2) detect the diversion or replacement of pins; 3) estimate the plutonium mass; 4) estimate decay heat; and 5) determine the reactivity of spent fuel assemblies. The differential die-away self-interrogation (DDSI) instrument is one instrument that was assessed for years regarding its feasibility for robust, timely verification of spent fuel assemblies. The instrument was recently built and was tested using fresh fuel assemblies in a variety of configurations, including varying enrichment, neutron absorber content, and symmetry. The early die-away method, a multiplication determination method developed in simulation space, was successfully tested on the fresh fuel assembly data and determined multiplication with a root-mean-square (RMS) error of 2.9%. The experimental results were compared with MCNP simulations of the instrument as well. Low multiplication assemblies had agreement with an average RMS error of 0.2% in the singles count rate (i.e., total neutrons detected per second) and 3.4% in the doubles count rates (i.e., neutrons detected in coincidence per second). High-multiplication assemblies had agreement with an average RMS error of 4.1% in the singles and 13.3% in the doubles count rates.

Fresh Fuel Measurements With the Differential Die-Away Self-Interrogation Instrument

DOE Office of Scientific and Technical Information (OSTI.GOV)

Trahan, Alexis C.; Belian, Anthony P.; Swinhoe, Martyn T.

The purpose of the Next Generation Safeguards Initiative (NGSI)-Spent Fuel (SF) Project is to strengthen the technical toolkit of safeguards inspectors and/or other interested parties. Thus the NGSI-SF team is working to achieve the following technical goals more easily and efficiently than in the past using nondestructive assay measurements of spent fuel assemblies: 1) verify the initial enrichment, burnup, and cooling time of facility declaration; 2) detect the diversion or replacement of pins; 3) estimate the plutonium mass; 4) estimate decay heat; and 5) determine the reactivity of spent fuel assemblies. The differential die-away self-interrogation (DDSI) instrument is one instrumentmore » that was assessed for years regarding its feasibility for robust, timely verification of spent fuel assemblies. The instrument was recently built and was tested using fresh fuel assemblies in a variety of configurations, including varying enrichment, neutron absorber content, and symmetry. The early die-away method, a multiplication determination method developed in simulation space, was successfully tested on the fresh fuel assembly data and determined multiplication with a root-mean-square (RMS) error of 2.9%. The experimental results were compared with MCNP simulations of the instrument as well. Low multiplication assemblies had agreement with an average RMS error of 0.2% in the singles count rate (i.e., total neutrons detected per second) and 3.4% in the doubles count rates (i.e., neutrons detected in coincidence per second). High-multiplication assemblies had agreement with an average RMS error of 4.1% in the singles and 13.3% in the doubles count rates.« less

Fresh Fuel Measurements With the Differential Die-Away Self-Interrogation Instrument

DOE PAGES

Trahan, Alexis C.; Belian, Anthony P.; Swinhoe, Martyn T.; ...

2017-01-05

The purpose of the Next Generation Safeguards Initiative (NGSI)-Spent Fuel (SF) Project is to strengthen the technical toolkit of safeguards inspectors and/or other interested parties. Thus the NGSI-SF team is working to achieve the following technical goals more easily and efficiently than in the past using nondestructive assay measurements of spent fuel assemblies: 1) verify the initial enrichment, burnup, and cooling time of facility declaration; 2) detect the diversion or replacement of pins; 3) estimate the plutonium mass; 4) estimate decay heat; and 5) determine the reactivity of spent fuel assemblies. The differential die-away self-interrogation (DDSI) instrument is one instrumentmore » that was assessed for years regarding its feasibility for robust, timely verification of spent fuel assemblies. The instrument was recently built and was tested using fresh fuel assemblies in a variety of configurations, including varying enrichment, neutron absorber content, and symmetry. The early die-away method, a multiplication determination method developed in simulation space, was successfully tested on the fresh fuel assembly data and determined multiplication with a root-mean-square (RMS) error of 2.9%. The experimental results were compared with MCNP simulations of the instrument as well. Low multiplication assemblies had agreement with an average RMS error of 0.2% in the singles count rate (i.e., total neutrons detected per second) and 3.4% in the doubles count rates (i.e., neutrons detected in coincidence per second). High-multiplication assemblies had agreement with an average RMS error of 4.1% in the singles and 13.3% in the doubles count rates.« less

Tripartite assembly of RND multidrug efflux pumps

NASA Astrophysics Data System (ADS)

Daury, Laetitia; Orange, François; Taveau, Jean-Christophe; Verchère, Alice; Monlezun, Laura; Gounou, Céline; Marreddy, Ravi K. R.; Picard, Martin; Broutin, Isabelle; Pos, Klaas M.; Lambert, Olivier

2016-02-01

Tripartite multidrug efflux systems of Gram-negative bacteria are composed of an inner membrane transporter, an outer membrane channel and a periplasmic adaptor protein. They are assumed to form ducts inside the periplasm facilitating drug exit across the outer membrane. Here we present the reconstitution of native Pseudomonas aeruginosa MexAB-OprM and Escherichia coli AcrAB-TolC tripartite Resistance Nodulation and cell Division (RND) efflux systems in a lipid nanodisc system. Single-particle analysis by electron microscopy reveals the inner and outer membrane protein components linked together via the periplasmic adaptor protein. This intrinsic ability of the native components to self-assemble also leads to the formation of a stable interspecies AcrA-MexB-TolC complex suggesting a common mechanism of tripartite assembly. Projection structures of all three complexes emphasize the role of the periplasmic adaptor protein as part of the exit duct with no physical interaction between the inner and outer membrane components.

Transport of LCLS-II 1.3 Ghz cryomodule to SLAC

DOE Office of Scientific and Technical Information (OSTI.GOV)

McGee, M. W.; Arkan, T.; Peterson, T.

2016-06-30

In a partnership with SLAC National Accelerator Laboratory (SLAC) and Jefferson Lab, Fermilab will assemble and test 17 of the 35 total 1.3 GHz cryomodules for the Linac Coherent Light Source II (LCLS-II) Project. These include a prototype built and delivered by each Lab. Another two 3.9 GHz cryomodules will be built, tested and transported by Fermilab to SLAC. Each assembly will be transported over-the-road from Fermilab or Jefferson Lab using specific routes to SLAC. The transport system consists of a base frame, isolation fixture and upper protective truss. The strongback cryomodule lifting fixture is described along with other supportingmore » equipment used for both over-the-road transport and local (on-site) transport at Fermilab. Initially, analysis of fragile components and stability studies will be performed in order to assess the risk associated with over-the-road transport of a fully assembled cryomodule.« less

Single-molecule sequencing of the desiccation-tolerant grass Oropetium thomaeum.

PubMed

VanBuren, Robert; Bryant, Doug; Edger, Patrick P; Tang, Haibao; Burgess, Diane; Challabathula, Dinakar; Spittle, Kristi; Hall, Richard; Gu, Jenny; Lyons, Eric; Freeling, Michael; Bartels, Dorothea; Ten Hallers, Boudewijn; Hastie, Alex; Michael, Todd P; Mockler, Todd C

2015-11-26

Plant genomes, and eukaryotic genomes in general, are typically repetitive, polyploid and heterozygous, which complicates genome assembly. The short read lengths of early Sanger and current next-generation sequencing platforms hinder assembly through complex repeat regions, and many draft and reference genomes are fragmented, lacking skewed GC and repetitive intergenic sequences, which are gaining importance due to projects like the Encyclopedia of DNA Elements (ENCODE). Here we report the whole-genome sequencing and assembly of the desiccation-tolerant grass Oropetium thomaeum. Using only single-molecule real-time sequencing, which generates long (>16 kilobases) reads with random errors, we assembled 99% (244 megabases) of the Oropetium genome into 625 contigs with an N50 length of 2.4 megabases. Oropetium is an example of a 'near-complete' draft genome which includes gapless coverage over gene space as well as intergenic sequences such as centromeres, telomeres, transposable elements and rRNA clusters that are typically unassembled in draft genomes. Oropetium has 28,466 protein-coding genes and 43% repeat sequences, yet with 30% more compact euchromatic regions it is the smallest known grass genome. The Oropetium genome demonstrates the utility of single-molecule real-time sequencing for assembling high-quality plant and other eukaryotic genomes, and serves as a valuable resource for the plant comparative genomics community.

Active Glutaminase C Self-assembles into a Supratetrameric Oligomer That Can Be Disrupted by an Allosteric Inhibitor*

PubMed Central

Ferreira, Amanda Petrina Scotá; Cassago, Alexandre; Gonçalves, Kaliandra de Almeida; Dias, Marília Meira; Adamoski, Douglas; Ascenção, Carolline Fernanda Rodrigues; Honorato, Rodrigo Vargas; de Oliveira, Juliana Ferreira; Ferreira, Igor Monteze; Fornezari, Camila; Bettini, Jefferson; Oliveira, Paulo Sérgio Lopes; Paes Leme, Adriana Franco; Portugal, Rodrigo Villares; Ambrosio, Andre Luis Berteli; Dias, Sandra Martha Gomes

2013-01-01

The phosphate-dependent transition between enzymatically inert dimers into catalytically capable tetramers has long been the accepted mechanism for the glutaminase activation. Here, we demonstrate that activated glutaminase C (GAC) self-assembles into a helical, fiber-like double-stranded oligomer and propose a molecular model consisting of seven tetramer copies per turn per strand interacting via the N-terminal domains. The loop 321LRFNKL326 is projected as the major regulating element for self-assembly and enzyme activation. Furthermore, the previously identified in vivo lysine acetylation (Lys311 in humans, Lys316 in mouse) is here proposed as an important down-regulator of superoligomer assembly and protein activation. Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide, a known glutaminase inhibitor, completely disrupted the higher order oligomer, explaining its allosteric mechanism of inhibition via tetramer stabilization. A direct correlation between the tendency to self-assemble and the activity levels of the three mammalian glutaminase isozymes was established, with GAC being the most active enzyme while forming the longest structures. Lastly, the ectopic expression of a fiber-prone superactive GAC mutant in MDA-MB 231 cancer cells provided considerable proliferative advantages to transformed cells. These findings yield unique implications for the development of GAC-oriented therapeutics targeting tumor metabolism. PMID:23935106

Spacecraft capture and docking system

NASA Technical Reports Server (NTRS)

Kong, Kinyuen (Inventor); Rafeek, Shaheed (Inventor); Myrick, Thomas (Inventor)

2001-01-01

A system for capturing and docking an active craft to a passive craft has a first docking assembly on the active craft with a first contact member and a spike projecting outwardly, a second docking assembly on the passive craft having a second contact member and a flexible net deployed over a target area with an open mesh for capturing the end of the spike of the active craft, and a motorized net drive for reeling in the net and active craft to mate with the passive craft's docking assembly. The spike has extendable tabs to allow it to become engaged with the net. The net's center is coupled to a net spool for reeling in. An alignment funnel has inclined walls to guide the net and captured spike towards the net spool. The passive craft's docking assembly includes circumferentially spaced preload wedges which are driven to lock the wedges against the contact member of the active craft. The active craft's docking assembly includes a rotary table and drive for rotating it to a predetermined angular alignment position, and mating connectors are then engaged with each other. The system may be used for docking spacecraft in zero or low-gravity environments, as well as for docking underwater vehicles, docking of ancillary craft to a mother craft in subsonic flight, in-flight refueling systems, etc.

Molecular self assembly and chiral recognition of copper octacyanophthalocyanine on Au(111): Interplay of intermolecular and molecule-substrate interactions.

NASA Astrophysics Data System (ADS)

Sk, Rejaul; Dhara, Barun; Miller, Joel; Deshpande, Aparna

Submolecular resolution scanning tunneling microscopy (STM) of copper octacyanophthalocyanine, CuPc(CN)8, at 77 K demonstrates that these achiral molecules form a two dimensional (2D) tetramer-based self-assembly upon evaporation onto an atomically flat Au(111) substrate. They assemble in two different structurally chiral configurations upon adsorption on Au(111). Scanning tunneling spectroscopy (STS),acquired at 77 K, unveils the HOMO and LUMO energy levels of this self-assembly. Voltage dependent STM images show that each molecule in both the structurally chiral configurations individually becomes chiral by breaking the mirror symmetry due to the enhanced intermolecular dipolar coupling interaction at the LUMO energy while the individual molecules remain achiral at the HOMO energy and within the HOMO-LUMO gap. At the LUMO energy, the handedness of the each chiral molecule is decided by the direction of the dipolar coupling interaction in the tetramer unit cell. This preference for LUMO energy indicates that this chirality is purely electronic in nature and it manifests on top of the organizational chirality that is present in the self-assembly independent of the orbital energy. Supported by IISER Pune and DAE-BRNS, India (Project No. 2011/20/37C/17/BRNS).

Improved Anti-Submarine Warfare (ASW) Effectiveness MSSE Capstone Project

DTIC Science & Technology

2008-06-01

9 - Barrier System RMA Data Component MTBF MTTR Source Buoy 9,600 Hours Not Repairable During Mission [Ref 70, Lumpkin and Pazos , 2004...and Mayra Pazos , “Lifetime Statistics of Most Recent Drifter Deployments (2002-2003),” Global Drifter Program/ Drifter Data Assembly Center, NOAA

WASTE MINIMIZATION ASSESSMENT FOR A MANUFACTURER OF REFURBISHED RAILCAR ASSEMBLIES

EPA Science Inventory

The U.S. Environmental Protection Agency (EPA) has funded a pilot project to assist small- and medium-size manufacturers who want to minimize their generation of waste but who lack the expertise to do so. Waste Minimization Assessment Centers (WMACs) were established at selected ...

Learning Factory--Integrative E-Learning

ERIC Educational Resources Information Center

Steininger, Peter

2017-01-01

Integrative E-Learning (IEP): The goal of the project is the development and testing of an integrative teaching format for the assembly of imported, tried and tested (classic) teaching elements (e.g. wallboard and flipchart, screen walls and other visualization aids, Projector, manuscripts, and workbooks, etc.), with contemporary, innovative…

Hypersonic research engine project. Phase 2: Aerothermodynamic integration model development

NASA Technical Reports Server (NTRS)

Jilly, L. F. (Editor)

1971-01-01

The fabrication of the various components of the HRE AIM was completed. The purge system necessary for the cavity bounded by the outer shell assembly and the outer cowl body was studied. Preparations were begun for establishing a format for test data acquisition and reduction.

Space Shuttle Projects

NASA Image and Video Library

1975-01-01

As early as September 1972, the Marshall Space Flight Center arnounced plans for a series of 20 water-entry simulation tests with a solid-fueled rocket casing assembly. The tests would provide valuable data for assessment of solid rocket booster parachute water recovery and aid in preliminary solid rocket motor design.

Western states one-stop shop for rural traveler information : research on Clarus system data.

DOT National Transportation Integrated Search

2011-09-01

Weather is a primary item of interest to travelers, but generally scattered numerous sources and in varying formats, making it difficult to assemble prior travel. Within this project, a website was developed to display Clarus ESS data, along with oth...

Teaching Mathematics Using Steplets

ERIC Educational Resources Information Center

Bringslid, Odd; Norstein, Anne

2008-01-01

This article evaluates online mathematical content used for teaching mathematics in engineering classes and in distance education for teacher training students. In the EU projects Xmath and dMath online computer algebra modules (Steplets) for undergraduate students assembled in the Xmath eBook have been designed. Two questionnaires, a compulsory…

SSME structural dynamic model development

NASA Technical Reports Server (NTRS)

Foley, M. J.; Tilley, D. M.; Welch, C. T.

1983-01-01

A mathematical model of the Space Shuttle Main Engine (SSME) as a complete assembly, with detailed emphasis on LOX and High Fuel Turbopumps is developed. The advantages of both complete engine dynamics, and high fidelity modeling are incorporated. Development of this model, some results, and projected applications are discussed.

Detail, oblique view to northeast atop building 935 showing exposed ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Detail, oblique view to northeast atop building 935 showing exposed roof sections of buildings 934 (center) and 933 (left), 90 mm lens. - Travis Air Force Base, Nuclear Weapons Assembly Plant 3, W Street, Armed Forces Special Weapons Project Q Area, Fairfield, Solano County, CA

SELF-ASSEMBLY CARBON NANOTUBES IN A MICROTRAP FOR ON-LINE PRECONCENTRATION OF VOLATILE ORGANICS. (R830901)

EPA Science Inventory

The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

Collaboration between Writers and Graphic Designers in Documentation Projects.

ERIC Educational Resources Information Center

Mirel, Barbara; And Others

1995-01-01

Analyzes collaborations between software manual writers and graphic designers to discover how their processes of collaboration directly affect the form of a finished manual. Identifies three models of collaboration: assembly line (linear drafting), swap meet (iterative drafting and joint problem solving), and symphony (codevelopment in every…

Atomic-Scale Tuning of Layered Binary Metal Oxides for High Temperature Moving Assemblies

DTIC Science & Technology

2015-06-01

AFRL-OSR-VA-TR-2015-0166 Atomic-Scale Tuning of Layered Binary Metal OxideS ASHLIE MARTINI UNIVERSITY OF CALIFORNIA MERCED Final Report 06/01/2015...Assemblies 5a.  CONTRACT NUMBER 5b.  GRANT NUMBER FA9550-12-1-0221 5c.  PROGRAM ELEMENT NUMBER 6.  AUTHOR(S) ASHLIE MARTINI 5d.  PROJECT NUMBER 5e...ABSTRACT UU 18.  NUMBER        OF        PAGES 19a.  NAME OF RESPONSIBLE PERSON ASHLIE MARTINI Standard Form 298 (Rev. 8/98) Prescribed by ANSI Std. Z39.18

On-Orbit Autonomous Assembly from Nanosatellites

NASA Technical Reports Server (NTRS)

Murchison, Luke S.; Martinez, Andres; Petro, Andrew

2015-01-01

The On-Orbit Autonomous Assembly from Nanosatellites (OAAN) project will demonstrate autonomous control algorithms for rendezvous and docking maneuvers; low-power reconfigurable magnetic docking technology; and compact, lightweight and inexpensive precision relative navigation using carrier-phase differential (CD) GPS with a three-degree of freedom ground demonstration. CDGPS is a specific relative position determination method that measures the phase of the GPS carrier wave to yield relative position data accurate to.4 inch (1 centimeter). CDGPS is a technology commonly found in the surveying industry. The development and demonstration of these technologies will fill a current gap in the availability of proven autonomous rendezvous and docking systems for small satellites.

Chapter I: Twenty Eighth General Assembly

NASA Astrophysics Data System (ADS)

Montmerle, Thierry

2015-08-01

Welcome to Beijing and IAU XXVIII General Assembly. Although the world economic situation continues to show stress, the science of astronomy is flourishing on many fronts. True, we are not immune to the negative effects of sharply reduced funding for projects and positions, yet our do-main sees increased international collaboration, pioneering facilities and techniques in development, and significant discoveries that are changing the way humanity thinks about the universe and our place in it. Programs that the IAU has undertaken such as the United Nations International Year of Astronomy 2009 and the creation of the Office of Astronomy for Development in Cape Town have been hugely successful.

Method of joining metallic and composite components

NASA Technical Reports Server (NTRS)

Semmes, Edmund B. (Inventor)

2010-01-01

A method is provided for joining a metallic member to a structure made of a composite matrix material. One or more surfaces of a portion of the metallic member that is to be joined to the composite matrix structure is provided with a plurality of outwardly projecting studs. The surface including the studs is brought into engagement with a portion of an uncured composite matrix material so that fibers of the composite matrix material intertwine with the studs, and the metallic member and composite structure form an assembly. The assembly is then companion cured so as to join the metallic member to the composite matrix material structure.

Turbine assembly containing an inner shroud

DOEpatents

Bagepalli, Bharat Sampathkumaran; Corman, Gregory Scot; Dean, Anthony John; DiMascio, Paul Stephen; Mirdamadi, Massoud

2000-01-01

A turbine assembly having a turbine stator, a ceramic inner shroud, and a first spring. The stator has a longitudinal axis and an outer shroud block with opposing and longitudinally outward facing first and second sides. The first side has a longitudinally outward projecting first ledge and has a first side portion located radially outward of the first ledge. The ceramic inner shroud has a first hook portion longitudinally and radially surrounding the first ledge. The first spring is attached to one of the first side portion and the first hook portion and unattachedly and resiliently contacts the other of the first side portion and the first hook portion.

Fiber optic engine for micro projection display.

PubMed

Arabi, Hesam Edin; An, Sohee; Oh, Kyunghwan

2010-03-01

A novel compact optical engine for a micro projector display is experimentally demonstrated, which is composed of RGB light sources, a tapered 3 x 1 Fiber Optic Color Synthesizer (FOCS) along with a fiberized ball-lens, and a two dimensional micro electromechanical scanning mirror. In the proposed optical engine, we successfully employed an all-fiber beam shaping technique combining optical fiber taper and fiberized ball lens that can render a narrow beam and enhance the resolution of the screened image in the far field. Optical performances of the proposed device assembly are investigated in terms of power loss, collimating strength of the collimator assembly, and color gamut of the output.

Calculating Trajectories And Orbits

NASA Technical Reports Server (NTRS)

Alderson, Daniel J.; Brady, Franklyn H.; Breckheimer, Peter J.; Campbell, James K.; Christensen, Carl S.; Collier, James B.; Ekelund, John E.; Ellis, Jordan; Goltz, Gene L.; Hintz, Gerarld R.;

Galaxy and Mass Assembly (GAMA): the red fraction and radial distribution of satellite galaxies

NASA Astrophysics Data System (ADS)

Prescott, Matthew; Baldry, I. K.; James, P. A.; Bamford, S. P.; Bland-Hawthorn, J.; Brough, S.; Brown, M. J. I.; Cameron, E.; Conselice, C. J.; Croom, S. M.; Driver, S. P.; Frenk, C. S.; Gunawardhana, M.; Hill, D. T.; Hopkins, A. M.; Jones, D. H.; Kelvin, L. S.; Kuijken, K.; Liske, J.; Loveday, J.; Nichol, R. C.; Norberg, P.; Parkinson, H. R.; Peacock, J. A.; Phillipps, S.; Pimbblet, K. A.; Popescu, C. C.; Robotham, A. S. G.; Sharp, R. G.; Sutherland, W. J.; Taylor, E. N.; Tuffs, R. J.; van Kampen, E.; Wijesinghe, D.

2011-10-01

We investigate the properties of satellite galaxies that surround isolated hosts within the redshift range 0.01 < z < 0.15, using data taken as part of the Galaxy And Mass Assembly survey. Making use of isolation and satellite criteria that take into account stellar mass estimates, we find 3514 isolated galaxies of which 1426 host a total of 2998 satellites. Separating the red and blue populations of satellites and hosts, using colour-mass diagrams, we investigate the radial distribution of satellite galaxies and determine how the red fraction of satellites varies as a function of satellite mass, host mass and the projected distance from their host. Comparing the red fraction of satellites to a control sample of small neighbours at greater projected radii, we show that the increase in red fraction is primarily a function of host mass. The satellite red fraction is about 0.2 higher than the control sample for hosts with ?, while the red fractions show no difference for hosts with ?. For the satellites of more massive hosts, the red fraction also increases as a function of decreasing projected distance. Our results suggest that the likely main mechanism for the quenching of star formation in satellites hosted by isolated galaxies is strangulation.

Molecular architecture of the human Mediator-RNA polymerase II-TFIIF assembly.

PubMed

Bernecky, Carrie; Grob, Patricia; Ebmeier, Christopher C; Nogales, Eva; Taatjes, Dylan J

2011-03-01

The macromolecular assembly required to initiate transcription of protein-coding genes, known as the Pre-Initiation Complex (PIC), consists of multiple protein complexes and is approximately 3.5 MDa in size. At the heart of this assembly is the Mediator complex, which helps regulate PIC activity and interacts with the RNA polymerase II (pol II) enzyme. The structure of the human Mediator-pol II interface is not well-characterized, whereas attempts to structurally define the Mediator-pol II interaction in yeast have relied on incomplete assemblies of Mediator and/or pol II and have yielded inconsistent interpretations. We have assembled the complete, 1.9 MDa human Mediator-pol II-TFIIF complex from purified components and have characterized its structural organization using cryo-electron microscopy and single-particle reconstruction techniques. The orientation of pol II within this assembly was determined by crystal structure docking and further validated with projection matching experiments, allowing the structural organization of the entire human PIC to be envisioned. Significantly, pol II orientation within the Mediator-pol II-TFIIF assembly can be reconciled with past studies that determined the location of other PIC components relative to pol II itself. Pol II surfaces required for interacting with TFIIB, TFIIE, and promoter DNA (i.e., the pol II cleft) are exposed within the Mediator-pol II-TFIIF structure; RNA exit is unhindered along the RPB4/7 subunits; upstream and downstream DNA is accessible for binding additional factors; and no major structural re-organization is necessary to accommodate the large, multi-subunit TFIIH or TFIID complexes. The data also reveal how pol II binding excludes Mediator-CDK8 subcomplex interactions and provide a structural basis for Mediator-dependent control of PIC assembly and function. Finally, parallel structural analysis of Mediator-pol II complexes lacking TFIIF reveal that TFIIF plays a key role in stabilizing pol II orientation within the assembly.

Molecular Architecture of the Human Mediator–RNA Polymerase II–TFIIF Assembly

PubMed Central

Bernecky, Carrie; Grob, Patricia; Ebmeier, Christopher C.; Nogales, Eva; Taatjes, Dylan J.

2011-01-01

The macromolecular assembly required to initiate transcription of protein-coding genes, known as the Pre-Initiation Complex (PIC), consists of multiple protein complexes and is approximately 3.5 MDa in size. At the heart of this assembly is the Mediator complex, which helps regulate PIC activity and interacts with the RNA polymerase II (pol II) enzyme. The structure of the human Mediator–pol II interface is not well-characterized, whereas attempts to structurally define the Mediator–pol II interaction in yeast have relied on incomplete assemblies of Mediator and/or pol II and have yielded inconsistent interpretations. We have assembled the complete, 1.9 MDa human Mediator–pol II–TFIIF complex from purified components and have characterized its structural organization using cryo-electron microscopy and single-particle reconstruction techniques. The orientation of pol II within this assembly was determined by crystal structure docking and further validated with projection matching experiments, allowing the structural organization of the entire human PIC to be envisioned. Significantly, pol II orientation within the Mediator–pol II–TFIIF assembly can be reconciled with past studies that determined the location of other PIC components relative to pol II itself. Pol II surfaces required for interacting with TFIIB, TFIIE, and promoter DNA (i.e., the pol II cleft) are exposed within the Mediator–pol II–TFIIF structure; RNA exit is unhindered along the RPB4/7 subunits; upstream and downstream DNA is accessible for binding additional factors; and no major structural re-organization is necessary to accommodate the large, multi-subunit TFIIH or TFIID complexes. The data also reveal how pol II binding excludes Mediator–CDK8 subcomplex interactions and provide a structural basis for Mediator-dependent control of PIC assembly and function. Finally, parallel structural analysis of Mediator–pol II complexes lacking TFIIF reveal that TFIIF plays a key role in stabilizing pol II orientation within the assembly. PMID:21468301

Nanoparticles Stabilize Thin Polymer Films: A Fundamental Study to Understand the Phenomenon

DOE Office of Scientific and Technical Information (OSTI.GOV)

Michael E. Mackay

2009-03-04

A new understanding of thermodynamics at the nanoscale resulted in a recently discovered first order phase transition that nanoparticles in a polymer film will all segregate to the supporting substrate. This is an unusual phase transition that was predicted using a modeling technique developed at Sandia National Laboratories and required the equivalent of many computational years on one computer. This project is a collaboration between Prof. Michael Mackay's group and Dr. Amalie Frischknecht (Sandia National Laboratories) where experimental observation and theoretical rationalization and prediction are brought together. Other discoveries were that this phase transition could be avoided by changing themore » nanoparticle properties yielding control of the assembly process at the nanoscale. In fact, the nanoparticles could be made to assemble to the supporting substrate, to the air interface or not assemble at all within a thin polymer film of order 100 nm in thickness. However, when the assembly process is present it is so robust that it is possible to make rough liquid films at the nanoscale due to nanoparticles assembling around three-dimensional objects. From this knowledge we are able to design and manufacture new coatings with particular emphasis on polymer-based solar cells. Careful control of the morphology at the nanoscale is expected to provide more efficient devices since the physics of these systems is dictated at this length scale and assembly of nanoparticles to various interfaces is critical to operation.« less

Assembly reliability of CSPs with various chiip sizes by accelerated thermal and mechanical cycling test

NASA Technical Reports Server (NTRS)

Ghaffarian, R.

2000-01-01

A JPL-led chip scale package (CSP) Consortium, composed of team members representing government agencies and private companies, recently joined together to pool in-kind resources for developing the quality and reliability of chip scale packages (CSPs) for a variety of projects.

Blue Origin testing

NASA Image and Video Library

2012-04-20

NASA Administrator Charles Bolden (r) discusses the upcoming testing of Blue Origin's BE-3 engine thrust chamber assembly with Steve Knowles, Blue Origin project manager, at the E-1 Test Stand during an April 20, 2012, visit to Stennis Space Center. Blue Origin is one of NASA's partners developing innovative systems to reach low-Earth orbit.

Stop the Drinking Driver: A Behavioral School-Based Prevention Program.

ERIC Educational Resources Information Center

Yates, Bruce A.; Dowrick, Peter W.

1991-01-01

Presents drinking and driving project focusing on friends and peers of high risk teenage drivers using modeling, positive peer pressure, and assertive skills training. Program includes schoolwide assembly and classroom development of strategies to prevent friends from drinking and driving. Evaluation survey results indicated that majority of…

Destination Innovation: Episode 2 SOFIA: Window to the Hidden Universe

NASA Image and Video Library

2012-03-21

Destination Innovation is a new series that explores the research, science and other projects underway at NASA's Ames Research Center. Episode 2 focuses on SOFIA, a highly modified Boeing 747SP aircraft with a 17-ton telescope assembly allowing scientists to peer deeper into the universe than ever before.

Phase 2 of the Array Automated Assembly Task for the Low Cost Silicon Solar Array Project

NASA Technical Reports Server (NTRS)

Wihl, M.; Torro, J.; Scheinine, A.; Anderson, J.

1978-01-01

An automated process sequence, to manufacture photovoltaic modules at a capacity of approximately 500 MW per year at a cost of approximately $0.50 per peak watt is described. Verification tests were performed and are reported along with cost predictions.

EXPRESSION AND SELF-ASSEMBLY OF NORWALK VIRUS CAPSID PROTEIN FROM VENEZUELAN EQUINE ENCEPHALITIS VIRUS REPLICONS. (R826139)

EPA Science Inventory

The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

7 CFR 947.30 - Duties.

Code of Federal Regulations, 2011 CFR

2011-01-01

... fiscal period, to meet and organize, to select a chairman and such other officers as may be necessary, to... duties of each such person; (e) To investigate, from time to time, and to assemble data on the growing... the establishment of marketing research and development projects pursuant to § 947.47. ...

7 CFR 947.30 - Duties.

Code of Federal Regulations, 2014 CFR

2014-01-01

... fiscal period, to meet and organize, to select a chairman and such other officers as may be necessary, to... duties of each such person; (e) To investigate, from time to time, and to assemble data on the growing... the establishment of marketing research and development projects pursuant to § 947.47. ...

7 CFR 947.30 - Duties.

Code of Federal Regulations, 2012 CFR

2012-01-01

... fiscal period, to meet and organize, to select a chairman and such other officers as may be necessary, to... duties of each such person; (e) To investigate, from time to time, and to assemble data on the growing... the establishment of marketing research and development projects pursuant to § 947.47. ...

7 CFR 947.30 - Duties.

Code of Federal Regulations, 2013 CFR

2013-01-01

... fiscal period, to meet and organize, to select a chairman and such other officers as may be necessary, to... duties of each such person; (e) To investigate, from time to time, and to assemble data on the growing... the establishment of marketing research and development projects pursuant to § 947.47. ...

Factory Production Occupations. Reprinted from the Occupational Outlook Handbook, 1978-79 Edition.

ERIC Educational Resources Information Center

Bureau of Labor Statistics (DOL), Washington, DC.

Focusing on factory production occupations, this document is one in a series of forty-one reprints from the Occupational Outlook Handbook providing current information and employment projections for individual occupations and industries through 1985. The specific occupations covered in this document include assemblers, blue collar worker…

Service, Sex Work, and the Profession

ERIC Educational Resources Information Center

Potts, Donna L.

2012-01-01

The author serves as the chair of the American Association of University Professors' (AAUP) Assembly of State Conferences, the umbrella organization for individual state AAUP conferences; a moderator for Pandora's Project, a website for survivors of sexual abuse and assault; a volunteer for Veronica's Voice, a support program for women and girls…

CRITICAL MICELLLE DENSITY FOR THE SELF-ASSEMBLY OF BLOCK COPOLYMER SURFACTANTS IN SUPERCRITICAL CARBON DIOXIDE. (R826115)

EPA Science Inventory

The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

The testes transcriptome derived from the New World Screwworm, Cochliomyia hominivorax SRA

USDA-ARS?s Scientific Manuscript database

In a collaboration with National Center for Genome Resources researchers, we sequenced and assembled the testes transcriptome derived from the Pacora, Panama, production plant strain J06 of the New World Screwworm, Cochliomyia hominivorax. This sequencing project produced 72,750,822 raw reads and th...

Genome sequencing of the redbanded stink bug (Piezodorus guildinii)

USDA-ARS?s Scientific Manuscript database

We assembled a partial genome sequence from the redbanded stink bug, Piezodorus guildinii from Illumina MiSeq sequencing runs. The sequence has been submitted and published under NCBI GenBank Accession Number JTEQ01000000. The BioProject and BioSample Accession numbers are PRJNA263369 and SAMN030997...

78 FR 69517 - U.S. Advisory Commission on Public Diplomacy; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-19

... Commission may conduct studies, inquiries, and meetings, as it deems necessary. It may assemble and..., in consultation with the Executive Director. The Advisory Commission may undertake foreign travel in pursuit of its studies and coordinate, sponsor, or oversee projects, studies, events, or other activities...

Teaching Information Systems Development via Process Variants

ERIC Educational Resources Information Center

Tan, Wee-Kek; Tan, Chuan-Hoo

2010-01-01

Acquiring the knowledge to assemble an integrated Information System (IS) development process that is tailored to the specific needs of a project has become increasingly important. It is therefore necessary for educators to impart to students this crucial skill. However, Situational Method Engineering (SME) is an inherently complex process that…

[Science and Technology and Recycling: Instructional Materials on Aluminum.

ERIC Educational Resources Information Center

Aluminum Association, New York, NY.

Educational materials on the manufacture and use of aluminum are assembled in this multi-media unit for use by junior high and secondary school students. Student booklets and brochures include: "The Story of Aluminum,""Uses of Aluminum,""Independent Study Guide for School Research Projects,""Questions and Answers…

Essentials of Nutrition Education in Medical Schools: A National Consensus.

ERIC Educational Resources Information Center

Academic Medicine, 1996

1996-01-01

The American Medical Student Association's Nutrition Curriculum Project assembled a 10-member advisory board to develop a comprehensive list of nutrition topics deemed essential for the adequate training of physicians. The resulting 92 topics are divided into 5 major categories: (1) biochemistry/physiology/pathophysiology; (2) nutrition…

DISCOVERY IN THE URBAN SPRAWL.

ERIC Educational Resources Information Center

HYMOVITZ, LEON

FOR A CULTURAL ENRICHMENT PROJECT ("DISCOVERY") IN A DISADVANTAGED PHILADELPIA HIGH SCHOOL, ATTENDANCE AT MUSIC, ART, AND THEATER EVENTS EARNED POINTS TOWARD A CERTIFICATE. THE STUDENTS ELECTED THE EVENTS FROM A PREPARED LIST OF ACTIVITIES, WHICH OFTEN WERE MADE PART OF THE ACADEMIC PROGRAM AND THE SCHOOL ASSEMBLIES. AS WELL AS OFFERING…

California mild CTV strains that break resistance in Trifoliate Orange

USDA-ARS?s Scientific Manuscript database

This is the final report of a project to characterize California isolates of Citrus tristeza virus (CTV) that replicate in Poncirus trifoliata (trifoliate orange). Next Generation Sequencing (NGS) of viral small interfering RNAs (siRNAs) and assembly of full-length sequences of mild California CTV i...

Minority Images: A Project Guide.

ERIC Educational Resources Information Center

Murphy, Sharon; Estrada, Paula

The reading list and assignments in this study guide are designed to involve high school students in examining the media from a nonwhite perspective. The beginning assignments require students to assemble their own bibliography relating to minority groups in the media, to examine the lives and contributions of specific Chicano, black, and American…

Photo radar speed enforcement in a state highway work zone : demonstration project Yeon Avenue.

DOT National Transportation Integrated Search

2010-04-01

The 2007 Oregon legislative assembly passed House Bill 2466, allowing the Oregon Department of Transportation to use photo radar in ODOT work zones on non-interstate state highways and required ODOT to report back to them on the safety impacts of thi...

Selected economic and demographic data for counties of the interior Columbia River Basin.

Treesearch

Wendy J. McGinnis

1996-01-01

A variety of county data have been assembled in support of the Interior Columbia River Basin Ecosystem Management Project. This research note is an effort to make some of the basic demographic and economic data available to the public for the counties involved in the assessment.

Radiation Test Results on COTS and non-COTS Electronic Devices for NASA-JSC Space Flight Projects

NASA Technical Reports Server (NTRS)

Allums, Kimberly K.; O'Neill, P. M.; Reddell, B. D.; Nguyen, K. V.; Bailey, C. R.

2012-01-01

This presentation reports the results of recent proton and heavy ion Single Event Effect (SEE) testing on a variety of COTS and non-COTs electronic devices and assemblies tested for the Space Shuttle, International Space Station (ISS) and Multi-Purpose Crew Vehicle (MPCV).

Building 933935, oblique view to southeast showing, left to right, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Building 933-935, oblique view to southeast showing, left to right, rear doors to building 933, 934, 935, 90 mm lens. Building 933 is in foreground. - Travis Air Force Base, Nuclear Weapons Assembly Plant 4, W Street, Armed Forces Special Weapons Project Q Area, Fairfield, Solano County, CA

10 CFR 34.3 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... stop, “J” tube and collimator when it is used as an exposure head. Becquerel (Bq) means one... (Projection sheath) means a flexible or rigid tube (i.e., “J” tube) for guiding the source assembly and the... materials by nondestructive methods, utilizing ionizing radiation to make radiographic images. Lay-barge...

10 CFR 34.3 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... stop, “J” tube and collimator when it is used as an exposure head. Becquerel (Bq) means one... (Projection sheath) means a flexible or rigid tube (i.e., “J” tube) for guiding the source assembly and the... materials by nondestructive methods, utilizing ionizing radiation to make radiographic images. Lay-barge...

10 CFR 34.3 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... stop, “J” tube and collimator when it is used as an exposure head. Becquerel (Bq) means one... (Projection sheath) means a flexible or rigid tube (i.e., “J” tube) for guiding the source assembly and the... materials by nondestructive methods, utilizing ionizing radiation to make radiographic images. Lay-barge...

Transfer Assembly Project, 2001: VCCS Transfer Rates.

ERIC Educational Resources Information Center

McHewitt, Earl R.; Taylor, Garry

This document discusses the transfer rates of students who entered Virginia's community colleges in the fall of 1995, using the methodology developed by the Center for the Study of Community Colleges. Numerous tables in the document include individual college rates with breakdowns by race/ethnicity and gender. College-specific transfer rates are…

"Job 1" at Ford: Employee Co-operation.

ERIC Educational Resources Information Center

Werther, William B., Jr.

1985-01-01

After describing the history of Ford Motor Company's relationship with workers from the inception of the assembly line through recent years, the author describes results of the 1979 National Joint Committee on Employee Involvement and 1982 labor negotiations, which resulted in retraining projects, tuition assistance, and career counseling and…

Building Teen Futures with Underwater Robotics

ERIC Educational Resources Information Center

Wallace, Michael L.; Freitas, William M.

2016-01-01

Preparing young Americans with science and technology skills has been on the forefront of educational reform for several years, and Extension has responded. Robotics projects have become a natural fit for 4-H clubs, with members' experiences ranging from using Lego® Mindstorms® and other "purchase and assemble" robotics kits to building…

49 CFR 177.835 - Class 1 materials.

Code of Federal Regulations, 2011 CFR

2011-10-01

... wheel base of less than 184 inches; (3) Any vehicle in the combination is a cargo tank which is required... Army, Navy or Air Force of the United States Government provided the Class 1 (explosive) materials are... boards, or any similar projections. (g) No detonator assembly or booster with detonator may be...

49 CFR 177.835 - Class 1 materials.

Code of Federal Regulations, 2012 CFR

2012-10-01

... wheel base of less than 184 inches; (3) Any vehicle in the combination is a cargo tank which is required... Army, Navy or Air Force of the United States Government provided the Class 1 (explosive) materials are... boards, or any similar projections. (g) No detonator assembly or booster with detonator may be...

49 CFR 177.835 - Class 1 materials.

Code of Federal Regulations, 2010 CFR

2010-10-01

... wheel base of less than 184 inches; (3) Any vehicle in the combination is a cargo tank which is required... Army, Navy or Air Force of the United States Government provided the Class 1 (explosive) materials are... boards, or any similar projections. (g) No detonator assembly or booster with detonator may be...

49 CFR 177.835 - Class 1 materials.

Code of Federal Regulations, 2014 CFR

2014-10-01

... wheel base of less than 184 inches; (3) Any vehicle in the combination is a cargo tank which is required... Army, Navy or Air Force of the United States Government provided the Class 1 (explosive) materials are... boards, or any similar projections. (g) No detonator assembly or booster with detonator may be...

49 CFR 177.835 - Class 1 materials.

Code of Federal Regulations, 2013 CFR

2013-10-01

... wheel base of less than 184 inches; (3) Any vehicle in the combination is a cargo tank which is required... Army, Navy or Air Force of the United States Government provided the Class 1 (explosive) materials are... boards, or any similar projections. (g) No detonator assembly or booster with detonator may be...

The Transfer Rate: A Model of Consistency.

ERIC Educational Resources Information Center

Cohen, Arthur M.; Sanchez, Jorge R.

In 1989, the Transfer Assembly project was initiated by the Center for the Study of Community Colleges (CSCC) to determine the contribution of community colleges to students' progress towards baccalaureate degrees. In that year, 48 community colleges provided data on students, with transfers defined as students with no prior college experience who…

Gyro and Accelerometer Based Navigation System for a Mobile Autonomous Robot.

DTIC Science & Technology

1985-12-02

special thanks goes to our thesis advisor Dr. Matthew Kabrisky for having the confidence to turn us loose on this project. Additionally, we would...Wordmaster Word Processor 1 Wordstar Word Processor 1 Virtual Devices Robo A 6802 Cross Assembler 1 Modem 720 Communication Program 1 CP/M Operating

There Was No Ford Plant in Their Future.

ERIC Educational Resources Information Center

McMahon, John J.

1981-01-01

Describes "Operation What Now," a project initiated by Orange County Community College (OCCC) in Middleton, NY, in response to the closing of the community's Ford Motor Company assembly plant. Explains the steps taken by OCCC to assist the unemployed workers: organizing public meetings, strengthening communication, and providing career counseling.…

Working with Climate Projections to Estimate Disease Burden: Perspectives from Public Health.

PubMed

Conlon, Kathryn C; Kintziger, Kristina W; Jagger, Meredith; Stefanova, Lydia; Uejio, Christopher K; Konrad, Charles

2016-08-09

There is interest among agencies and public health practitioners in the United States (USA) to estimate the future burden of climate-related health outcomes. Calculating disease burden projections can be especially daunting, given the complexities of climate modeling and the multiple pathways by which climate influences public health. Interdisciplinary coordination between public health practitioners and climate scientists is necessary for scientifically derived estimates. We describe a unique partnership of state and regional climate scientists and public health practitioners assembled by the Florida Building Resilience Against Climate Effects (BRACE) program. We provide a background on climate modeling and projections that has been developed specifically for public health practitioners, describe methodologies for combining climate and health data to project disease burden, and demonstrate three examples of this process used in Florida.

Evaluation of the feasibility and viability of modular pumped storage hydro (m-PSH) in the United States

DOE Office of Scientific and Technical Information (OSTI.GOV)

Witt, Adam M.; Hadjerioua, Boualem; Martinez, Rocio

The viability of modular pumped storage hydro (m-PSH) is examined in detail through the conceptual design, cost scoping, and economic analysis of three case studies. Modular PSH refers to both the compactness of the project design and the proposed nature of product fabrication and performance. A modular project is assumed to consist of pre-fabricated standardized components and equipment, tested and assembled into modules before arrival on site. This technology strategy could enable m-PSH projects to deploy with less substantial civil construction and equipment component costs. The concept of m-PSH is technically feasible using currently available conventional pumping and turbine equipment,more » and may offer a path to reducing the project development cycle from inception to commissioning.« less

Working with Climate Projections to Estimate Disease Burden: Perspectives from Public Health

PubMed Central

Conlon, Kathryn C.; Kintziger, Kristina W.; Jagger, Meredith; Stefanova, Lydia; Uejio, Christopher K.; Konrad, Charles

2016-01-01

There is interest among agencies and public health practitioners in the United States (USA) to estimate the future burden of climate-related health outcomes. Calculating disease burden projections can be especially daunting, given the complexities of climate modeling and the multiple pathways by which climate influences public health. Interdisciplinary coordination between public health practitioners and climate scientists is necessary for scientifically derived estimates. We describe a unique partnership of state and regional climate scientists and public health practitioners assembled by the Florida Building Resilience Against Climate Effects (BRACE) program. We provide a background on climate modeling and projections that has been developed specifically for public health practitioners, describe methodologies for combining climate and health data to project disease burden, and demonstrate three examples of this process used in Florida. PMID:27517942

Genomes OnLine Database (GOLD) v.6: data updates and feature enhancements

PubMed Central

Mukherjee, Supratim; Stamatis, Dimitri; Bertsch, Jon; Ovchinnikova, Galina; Verezemska, Olena; Isbandi, Michelle; Thomas, Alex D.; Ali, Rida; Sharma, Kaushal; Kyrpides, Nikos C.; Reddy, T. B. K.

2017-01-01

The Genomes Online Database (GOLD) (https://gold.jgi.doe.gov) is a manually curated data management system that catalogs sequencing projects with associated metadata from around the world. In the current version of GOLD (v.6), all projects are organized based on a four level classification system in the form of a Study, Organism (for isolates) or Biosample (for environmental samples), Sequencing Project and Analysis Project. Currently, GOLD provides information for 26 117 Studies, 239 100 Organisms, 15 887 Biosamples, 97 212 Sequencing Projects and 78 579 Analysis Projects. These are integrated with over 312 metadata fields from which 58 are controlled vocabularies with 2067 terms. The web interface facilitates submission of a diverse range of Sequencing Projects (such as isolate genome, single-cell genome, metagenome, metatranscriptome) and complex Analysis Projects (such as genome from metagenome, or combined assembly from multiple Sequencing Projects). GOLD provides a seamless interface with the Integrated Microbial Genomes (IMG) system and supports and promotes the Genomic Standards Consortium (GSC) Minimum Information standards. This paper describes the data updates and additional features added during the last two years. PMID:27794040

Shipping device for heater unit assembly

DOEpatents

Blaushild, Ronald M.; Abbott, Stephan L.; Miller, Phillip E.; Shaffer, Robert

1991-01-01

A shipping device for a heater unit assembly (23), the heater unit assembly (23) including a cylindrical wall (25) and a top plate (31) secured to the cylindrical wall (25) and having a flange portion which projects radially beyond the outer surface of the cylindrical wall (25), and the shipping device including: a cylindrical container (3) having a closed bottom (13); a support member (47) secured to the container (3) and having an inwardly directed flange for supporting the flange portion of the top plate (31); a supplemental supporting system (1) for positioning the heater unit assembly (23) in the container (3) at a spaced relation from the inner surface and bottom wall (13) of the container (3); a cover (15) for closing the top of the container (3); and a container supporting structure (5,7,8) supporting the container (3) in a manner to permit the container (3) to be moved, relative to the supporting structure (5,7,8 ), between a vertical position for loading and unloading the assembly (23) and a horizontal position for transport of the assembly (23). A seal (57) is interposed between the container (3) and the cover (15) for sealing the interior of the container (3) from the environment. An abutment member (41) is mounted on the container supporting structure (5,7,8) for supporting the container bottom (13), when the container (3) is in the vertical position, to prevent the container (3) from moving past the vertical position in the direction away from the horizontal position, and a retainer member (55) is secured within the cover (15) for retaining the assembly top plate (31) in contact with the support member (47) when the cover (15) closes the top of the container (3).

Accelerator-based BNCT.

PubMed

Kreiner, A J; Baldo, M; Bergueiro, J R; Cartelli, D; Castell, W; Thatar Vento, V; Gomez Asoia, J; Mercuri, D; Padulo, J; Suarez Sandin, J C; Erhardt, J; Kesque, J M; Valda, A A; Debray, M E; Somacal, H R; Igarzabal, M; Minsky, D M; Herrera, M S; Capoulat, M E; Gonzalez, S J; del Grosso, M F; Gagetti, L; Suarez Anzorena, M; Gun, M; Carranza, O

2014-06-01

The activity in accelerator development for accelerator-based BNCT (AB-BNCT) both worldwide and in Argentina is described. Projects in Russia, UK, Italy, Japan, Israel, and Argentina to develop AB-BNCT around different types of accelerators are briefly presented. In particular, the present status and recent progress of the Argentine project will be reviewed. The topics will cover: intense ion sources, accelerator tubes, transport of intense beams, beam diagnostics, the (9)Be(d,n) reaction as a possible neutron source, Beam Shaping Assemblies (BSA), a treatment room, and treatment planning in realistic cases. © 2013 Elsevier Ltd. All rights reserved.