Low temperature conversion of plastic waste into light hydrocarbons.

PubMed

Shah, Sajid Hussain; Khan, Zahid Mahmood; Raja, Iftikhar Ahmad; Mahmood, Qaisar; Bhatti, Zulfiqar Ahmad; Khan, Jamil; Farooq, Ather; Rashid, Naim; Wu, Donglei

2010-07-15

Advance recycling through pyrolytic technology has the potential of being applied to the management of plastic waste (PW). For this purpose 1 l volume, energy efficient batch reactor was manufactured locally and tested for pyrolysis of waste plastic. The feedstock for reactor was 50 g waste polyethylene. The average yield of the pyrolytic oil, wax, pyrogas and char from pyrolysis of PW were 48.6, 40.7, 10.1 and 0.6%, respectively, at 275 degrees C with non-catalytic process. Using catalyst the average yields of pyrolytic oil, pyrogas, wax and residue (char) of 50 g of PW was 47.98, 35.43, 16.09 and 0.50%, respectively, at operating temperature of 250 degrees C. The designed reactor could work at low temperature in the absence of a catalyst to obtain similar products as for a catalytic process. 2010 Elsevier B.V. All rights reserved.

Production and fuel characteristics of vegetable oil from oilseed crops in the Pacific Northwest

DOE Office of Scientific and Technical Information (OSTI.GOV)

Auld, D.L.; Bettis, B.L.; Peterson, C.L.

1982-01-01

The purpose of this research was to evaluate the potential yield and fuel quality of various oilseed crops adapted to the Pacific Northwest as a source of liquid fuel for diesel engines. The seed yield and oil production of three cultivars of winter rape (Brassica napus L.), two cultivars of safflower (Carthamus tinctorius L.) and two cultivars of sunflower (Helianthus annuus L.) were evaluated in replicated plots at Moscow. Additional trials were conducted at several locations in Idaho, Oregon and Washington. Sunflower, oleic and linoleic safflower, and low and high erucic acid rapeseed were evaluated for fatty acid composition, energymore » content, viscosity and engine performance in short term tests. During 20 minute engine tests power output, fuel economy and thermal efficiency were compared to diesel fuel. Winter rape produced over twice as much farm extractable oil as either safflower or sunflower. The winter rape cultivars, Norde and Jet Neuf had oil yields which averaged 1740 and 1540 L/ha, respectively. Vegetable oils contained 94 to 95% of the KJ/L of diesel fuel, but were 11.1 to 17.6 times more viscous. Viscosity of the vegetable oils was closely related to fatty acid chain length and number of unsaturated bonds (R/sup 2/=.99). During short term engine tests all vegetable oils produced power outputs equivalent to diesel, and had thermal efficiencies 1.8 to 2.8% higher than diesel. Based on these results it appears that species and cultivars of oilseed crops to be utilized as a source of fuel should be selected on the basis of oil yield. 1 figure, 5 tables.« less

Final Technical Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sara Bergan, Executive Director; Brendan Jordan, Program Manager; Subcontractors as listed on the report.

2007-06-06

The following report contributes to our knowledge of how to economically produce wildlife-friendly grass mixtures for future fuel feedstocks in the northern plains. It investigates northern-adapted cultivars; management and harvest regimes that are good for yields, soils and wildlife; comparative analysis of monocultures and simple mixtures of native grasses; economic implications of growing grasses for fuel feedstocks in specific locations in the northern plains; and conversion options for turning the grasses into useful chemicals and fuels. The core results of this study suggest the following; Native grasses, even simple grass mixtures, can be produced profitably in the northern plains asmore » far west as the 100th meridian with yields ranging from 2 to 6 tons per acre; Northern adapted cultivars may yield less in good years, but have much greater long-term sustainable yield potential than higher-yielding southern varieties; Grasses require very little inputs and stop economically responding to N applications above 56kg/hectare; Harvesting after a killing frost may reduce the yield available in that given year but will increase overall yields averaged throughout multiple years; Harvesting after a killing frost or even in early spring reduces the level of ash and undesirable molecules like K which cause adverse reactions in pyrolysis processing. Grasses can be managed for biomass harvest and maintain or improve overall soil-health and carbon sequestration benefits of idled grassland; The carbon sequestration activity of the grasses seems to follow the above ground health of the biomass. In other words plots where the above ground biomass is regularly removed can continue to sequester carbon at the rate of 2 tons/acre/year if the stand health is strong and yielding significant amounts of biomass; Managing grasses for feedstock quality in a biomass system requires some of the same management strategies as managing for wildlife benefit. We believe that biomass development can be done in such a way that also maximizes or improves upon conservation and other environmental goals (in some cases even when compared to idled land); Switchgrass and big bluestem work well together in simple mixture plots where big bluestem fills in around the switchgrass which alone grows in bunches and leaves patches of bare soil open and susceptible to erosion; Longer-term studies in the northern plains may also find that every other year harvest schemes produce as much biomass averaged over the years as annual harvests; Grasses can be grown for between $23 and $54/ton in the northern plains at production rates between 3 and 5 tons/acre; Land costs, yields, and harvest frequency are the largest determining factors in the farm scale economics. Without any land rent offset or incentive for production, and with annual harvesting, grass production is likely to be around $35/ton in the northern plains (farm gate); Average transportation costs range from $3 to $10/ton delivered to the plant gate. Average distance from the plant is the biggest factor - $3/ton at 10 miles, $10/ton at 50 miles; There is a substantial penalty paid on a per unit of energy produced basis when one converts grasses to bio-oil, but the bio-oil can then compete in higher priced fuel markets whereas grasses alone compete directly with relatively cheap coal; Bio oil or modified bio-oil (without the HA or other chemical fraction) is a suitable fuel for boiler and combustion turbines that would otherwise use residual fuel oil or number 2 diesel; Ensyn has already commercialized the use of HA in smokey flavorants for the food industry but that market is rather small. HA, however, is also found to be a suitable replacement for the much larger US market for ethanolamines and ethalyne oxides that are used as dispersants; Unless crude oil prices rise, the highest and best use of grass based bio-oil is primarily as a direct fuel. As prices rise, HA, phenol and other chemical fractions may become more attractive; Although we were able to create available glucose from the AHG fraction in the bio-oil it proved recalcitrant to fermentation by yeast. Although fermentation results were much more positive with wood based bio-oil sugars, ethanol does not appear to be a likely product from grass based bio-oil; and A package of policy recommendations has been developed with roughly 75 key stakeholders from throughout the region that would support the transition to greater development of advanced biofuels and products in the region, as well as a strong role for native grass agriculture to support those industries.« less

Polarimetric Decomposition Analysis of the Deepwater Horizon Oil Slick Using L-Band UAVSAR Data

NASA Technical Reports Server (NTRS)

Jones, Cathleen; Minchew, Brent; Holt, Benjamin

2011-01-01

We report here an analysis of the polarization dependence of L-band radar backscatter from the main slick of the Deepwater Horizon oil spill, with specific attention to the utility of polarimetric decomposition analysis for discrimination of oil from clean water and identification of variations in the oil characteristics. For this study we used data collected with the UAVSAR instrument from opposing look directions directly over the main oil slick. We find that both the Cloude-Pottier and Shannon entropy polarimetric decomposition methods offer promise for oil discrimination, with the Shannon entropy method yielding the same information as contained in the Cloude-Pottier entropy and averaged in tensity parameters, but with significantly less computational complexity

A sub-canopy structure for simulating oil palm in the Community Land Model (CLM-Palm): phenology, allocation and yield

NASA Astrophysics Data System (ADS)

Fan, Y.; Roupsard, O.; Bernoux, M.; Le Maire, G.; Panferov, O.; Kotowska, M. M.; Knohl, A.

2015-11-01

In order to quantify the effects of forests to oil palm conversion occurring in the tropics on land-atmosphere carbon, water and energy fluxes, we develop a new perennial crop sub-model CLM-Palm for simulating a palm plant functional type (PFT) within the framework of the Community Land Model (CLM4.5). CLM-Palm is tested here on oil palm only but is meant of generic interest for other palm crops (e.g., coconut). The oil palm has monopodial morphology and sequential phenology of around 40 stacked phytomers, each carrying a large leaf and a fruit bunch, forming a multilayer canopy. A sub-canopy phenological and physiological parameterization is thus introduced so that each phytomer has its own prognostic leaf growth and fruit yield capacity but with shared stem and root components. Phenology and carbon and nitrogen allocation operate on the different phytomers in parallel but at unsynchronized steps, separated by a thermal period. An important phenological phase is identified for the oil palm - the storage growth period of bud and "spear" leaves which are photosynthetically inactive before expansion. Agricultural practices such as transplanting, fertilization and leaf pruning are represented. Parameters introduced for the oil palm were calibrated and validated with field measurements of leaf area index (LAI), yield and net primary production (NPP) from Sumatra, Indonesia. In calibration with a mature oil palm plantation, the cumulative yields from 2005 to 2014 matched notably well between simulation and observation (mean percentage error = 3 %). Simulated inter-annual dynamics of PFT-level and phytomer-level LAI were both within the range of field measurements. Validation from eight independent oil palm sites shows the ability of the model to adequately predict the average leaf growth and fruit yield across sites and sufficiently represent the significant nitrogen- and age-related site-to-site variability in NPP and yield. Results also indicate that seasonal dynamics of yield and remaining small-scale site-to-site variability of NPP are driven by processes not yet implemented in the model or reflected in the input data. The new sub-canopy structure and phenology and allocation functions in CLM-Palm allow exploring the effects of tropical land-use change, from natural ecosystems to oil palm plantations, on carbon, water and energy cycles and regional climate.

Tertiary geology and oil-shale resources of the Piceance Creek basin between the Colorado and White Rivers, northwestern Colorado

USGS Publications Warehouse

Donnell, John R.

1961-01-01

The area of the Piceance Creek basin between the Colorado and White Rivers includes approximately 1,600 square miles and is characterized by an extensive plateau that rises 1,000 to more than 4,000 feet above the surrounding lowlands. Relief is greatest in Naval Oil-Shale Reserves Nos. 1 and 3 near the south margin of the area, where the spectacular Roan Cliffs tower above the valley of the Colorado River. The oldest rocks exposed in the mapped area are sandstone, shale, and coal beds of the Mesaverde group of Late Cretaceous age, which crop out along the east margin of the area. Overlying the Mesaverde is an unnamed sequence of dark-colored sandstone and shale, Paleocene in age. The Ohio Creek conglomerate, composed of black and red chert and quartzite pebbles in a white sandstone matrix, is probably the basal unit in the Paleocene sequence. The Wasatch formation of early Eocene age overlies the Paleocene sedimentary rocks. It is composed of brightly colored shale, lenticular beds of sandstone, and a few thin beds of fresh-water limestone. The Kasatch formation interfingers with and is overlain by the Green River formation of middle Eocene age. The Green River formation has been divided into the Douglas Creek, Garden Gulch, Anvil Points, Parachute Creek, and Evacuation Creek members. The basal and uppermost members, the Douglas Creek and Evacuation Creek, respectively, are predominantly sandy units. The two middle members, the Garden Gulch and Parachute Creek, are composed principally of finer clastic rocks. The Anvil Points member is present only on the southeast, east, and northeast margins of the area. It is a nearshore facies composed principally of sandstone and is the equivalent of the Douglas Creek, Garden Gulch, and the lower part of the Parachute Creek members. All of the richer exposed oil-shale beds are found in the Parachute Creek member, which is divided into two oil-shale zones by a series of low-grade oilshale beds. The upper oil-shale zone has several key beds and zones which can be traced throughout most of the mapped area. One of these, the Mahogany ledge or zone, is a group of very rich oil-shale beds at the base of the upper oil-shale zone. Drilling for oil and gas in the northeastern part of the area has revealed rich oil-shale zones in the Garden Gulch member also.Local unconformities within and at the base of the Evacuation Creek member are exposed at several places along Piceance Creek and at one place near the mouth of Yellow Creek; otherwise, the rock sequence is conformable. The mapped area is the major part of a large syncline, modified by numerous smaller structural features. Fractures, probably associated genetically with the minor structural features, are present in the central part of the area. These fractures are high-angle normal faults with small displacement. They occur in pairs with the intervening block downdropped. Two sets of joints are prominent, one trending northwest and the other northeast. The joint systems control the drainage pattern in the south-central part of the area. More than 20,000 feet of sedimentary rocks underlies the area. Many of the formations yield oil or gas in northwestern Colorado, northeastern Utah, and southwestern Wyoming. The Piceance Creek gas field, in which gas occurs in the Douglas Creek member of the Green River formation, is the largest oil or gas field discovered thus far within the area. About 7,000 million barrels of oil is contained in oil shale that yields an average of 45 gallons per ton from a continuous sequence 5 or more feet thick in the Mahogany zone. Oil shale in the Mahogany zone and adjacent beds that yields an average of 30 gallons of oil per ton from a continuous sequence 15 or more feet thick contains about 91,000 million barrels of oil. Similar shale in deeper zones in the northern part of the area, for which detailed estimates have not been prepared, are now known to contain at least an additional 72,000 million barrels of oil. Oil shale in a sequence 15 or more feet thick that yields an average of 25 gallons of oil per ton contains about 154,000 million barrels of oil in the Mahogany zone and adjacent beds; such shale in deeper zones in the northern part of the area probably contains at least an additional 157,000 million barrels of oil, although detailed estimates were not made. Oil shale in a sequence greater than 15 feet thick that yields an average of 15 gallons of oil per ton contains more than 900,000 million barrels of oil. These estimates of the oil content of the deposit do not take into account any loss in mining or processing of the shale.

Differentiation of essential oils in Atractylodes lancea and Atractylodes koreana by gas chromatography with mass spectrometry.

PubMed

Liu, Qiutao; Zhang, Shanshan; Yang, Xihui; Wang, Ruilin; Guo, Weiying; Kong, Weijun; Yang, Meihua

2016-12-01

Atractylodes rhizome is a valuable traditional Chinese medicinal herb that comprises complex several species whose essential oils are the primary pharmacologically active component. Essential oils of Atractylodes lancea and Atractylodes koreana were extracted by hydrodistillation, and the yield was determined. The average yield of essential oil obtained from A. lancea (2.91%) was higher than that from A. koreana (2.42%). The volatile components of the essential oils were then identified by a gas chromatography with mass spectrometry method that demonstrated good precision. The method showed clear differences in the numbers and contents of volatile components between the two species. 41 and 45 volatile components were identified in A. lancea and A. koreana, respectively. Atractylon (48.68%) was the primary volatile component in A. lancea, while eudesma-4(14)-en-11-ol (11.81%) was major in A. koreana. However, the most significant difference between A. lancea and A. koreana was the major component of atractylon and atractydin. Principal component analysis was utilized to reveal the correlation between volatile components and species, and the analysis was used to successfully discriminate between A. lancea and A. koreana samples. These results suggest that different species of Atractylodes rhizome may yield essential oils that differ significantly in content and composition. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

A sub-canopy structure for simulating oil palm in the Community Land Model: phenology, allocation and yield

NASA Astrophysics Data System (ADS)

Fan, Y.; Roupsard, O.; Bernoux, M.; Le Maire, G.; Panferov, O.; Kotowska, M. M.; Knohl, A.

2015-06-01

Land surface modelling has been widely used to characterize the two-way interactions between climate and human activities in terrestrial ecosystems such as deforestation, agricultural expansion, and urbanization. Towards an effort to quantify the effects of forests to oil palm conversion occurring in the tropics on land-atmosphere carbon, water and energy fluxes, we introduce a new perennial crop plant functional type (PFT) for oil palm. Due to the modular and sequential nature of oil palm growth (around 40 stacked phytomers) and yield (fruit bunches axillated on each phytomer), we developed a specific sub-canopy structure for simulating palm's growth and yield within the framework of the Community Land Model (CLM4.5). In this structure each phytomer has its own prognostic leaf growth and fruit yield capacity like a PFT but with shared stem and root components among all phytomers. Phenology and carbon and nitrogen allocation operate on the different phytomers in parallel but at unsynchronized steps, so that multiple fruit yields per annum are enabled in terms of carbon and nitrogen outputs. An important phenological phase is identified for the palm PFT - the storage growth period of bud and "spear" leaves which are photosynthetically inactive before expansion. Agricultural practices such as transplanting, fertilization, and leaf pruning are represented. Parameters introduced for the new PFT were calibrated and validated with field measurements of leaf area index (LAI) and yield from Sumatra, Indonesia. In calibration with a mature oil palm plantation, the cumulative yields from 2005 to 2014 matched perfectly between simulation and observation (mean percentage error = 4 %). Simulated inter-annual dynamics of PFT-level and phytomer-level LAI were both within the range of field measurements. Validation from eight independent oil palm sites shows the ability of the model to adequately predict the average leaf growth and fruit yield across sites but also indicates that seasonal dynamics and site-to-site variability of yield are driven by processes not yet implemented in the model. The new sub-canopy structure and phenology and allocation functions now allow exploring the effects of tropical land use change, from natural ecosystems to oil palm plantations, on carbon, water and energy cycles and regional climate.

Oil palm natural diversity and the potential for yield improvement

PubMed Central

Barcelos, Edson; Rios, Sara de Almeida; Cunha, Raimundo N. V.; Lopes, Ricardo; Motoike, Sérgio Y.; Babiychuk, Elena; Skirycz, Aleksandra; Kushnir, Sergei

2015-01-01

African oil palm has the highest productivity amongst cultivated oleaginous crops. Species can constitute a single crop capable to fulfill the growing global demand for vegetable oils, which is estimated to reach 240 million tons by 2050. Two types of vegetable oil are extracted from the palm fruit on commercial scale. The crude palm oil and kernel palm oil have different fatty acid profiles, which increases versatility of the crop in industrial applications. Plantations of the current varieties have economic life-span around 25–30 years and produce fruits around the year. Thus, predictable annual palm oil supply enables marketing plans and adjustments in line with the economic forecasts. Oil palm cultivation is one of the most profitable land uses in the humid tropics. Oil palm fruits are the richest plant source of pro-vitamin A and vitamin E. Hence, crop both alleviates poverty, and could provide a simple practical solution to eliminate global pro-vitamin A deficiency. Oil palm is a perennial, evergreen tree adapted to cultivation in biodiversity rich equatorial land areas. The growing demand for the palm oil threatens the future of the rain forests and has a large negative impact on biodiversity. Plant science faces three major challenges to make oil palm the key element of building the future sustainable world. The global average yield of 3.5 tons of oil per hectare (t) should be raised to the full yield potential estimated at 11–18t. The tree architecture must be changed to lower labor intensity and improve mechanization of the harvest. Oil composition should be tailored to the evolving needs of the food, oleochemical and fuel industries. The release of the oil palm reference genome sequence in 2013 was the key step toward this goal. The molecular bases of agronomically important traits can be and are beginning to be understood at the single base pair resolution, enabling gene-centered breeding and engineering of this remarkable crop. PMID:25870604

Oil palm natural diversity and the potential for yield improvement.

PubMed

Barcelos, Edson; Rios, Sara de Almeida; Cunha, Raimundo N V; Lopes, Ricardo; Motoike, Sérgio Y; Babiychuk, Elena; Skirycz, Aleksandra; Kushnir, Sergei

2015-01-01

African oil palm has the highest productivity amongst cultivated oleaginous crops. Species can constitute a single crop capable to fulfill the growing global demand for vegetable oils, which is estimated to reach 240 million tons by 2050. Two types of vegetable oil are extracted from the palm fruit on commercial scale. The crude palm oil and kernel palm oil have different fatty acid profiles, which increases versatility of the crop in industrial applications. Plantations of the current varieties have economic life-span around 25-30 years and produce fruits around the year. Thus, predictable annual palm oil supply enables marketing plans and adjustments in line with the economic forecasts. Oil palm cultivation is one of the most profitable land uses in the humid tropics. Oil palm fruits are the richest plant source of pro-vitamin A and vitamin E. Hence, crop both alleviates poverty, and could provide a simple practical solution to eliminate global pro-vitamin A deficiency. Oil palm is a perennial, evergreen tree adapted to cultivation in biodiversity rich equatorial land areas. The growing demand for the palm oil threatens the future of the rain forests and has a large negative impact on biodiversity. Plant science faces three major challenges to make oil palm the key element of building the future sustainable world. The global average yield of 3.5 tons of oil per hectare (t) should be raised to the full yield potential estimated at 11-18t. The tree architecture must be changed to lower labor intensity and improve mechanization of the harvest. Oil composition should be tailored to the evolving needs of the food, oleochemical and fuel industries. The release of the oil palm reference genome sequence in 2013 was the key step toward this goal. The molecular bases of agronomically important traits can be and are beginning to be understood at the single base pair resolution, enabling gene-centered breeding and engineering of this remarkable crop.

Optimization of experimental conditions for composite biodiesel production from transesterification of mixed oils of Jatropha and Pongamia

NASA Astrophysics Data System (ADS)

Yogish, H.; Chandrashekara, K.; Pramod Kumar, M. R.

2012-11-01

India is looking at the renewable alternative sources of energy to reduce its dependence on import of crude oil. As India imports 70 % of the crude oil, the country has been greatly affected by increasing cost and uncertainty. Biodiesel fuel derived by the two step acid transesterification of mixed non-edible oils from Jatropha curcas and Pongamia (karanja) can meet the requirements of diesel fuel in the coming years. In the present study, different proportions of Methanol, Sodium hydroxide, variation of Reaction time, Sulfuric acid and Reaction Temperature were adopted in order to optimize the experimental conditions for maximum biodiesel yield. The preliminary studies revealed that biodiesel yield varied widely in the range of 75-95 % using the laboratory scale reactor. The average yield of 95 % was obtained. The fuel and chemical properties of biodiesel, namely kinematic viscosity, specific gravity, density, flash point, fire point, calorific value, pH, acid value, iodine value, sulfur content, water content, glycerin content and sulfated ash values were found to be within the limits suggested by Bureau of Indian Standards (BIS 15607: 2005). The optimum combination of Methanol, Sodium hydroxide, Sulfuric acid, Reaction Time and Reaction Temperature are established.

Production of polyhydroxyalkanoates from spent coffee grounds oil obtained by supercritical fluid extraction technology.

PubMed

Cruz, Madalena V; Paiva, Alexandre; Lisboa, Pedro; Freitas, Filomena; Alves, Vítor D; Simões, Pedro; Barreiros, Susana; Reis, Maria A M

2014-04-01

Spent coffee grounds (SCG) oil was obtained by supercritical carbon dioxide (scCO2) extraction in a pilot plant apparatus, with an oil extraction yield of 90% at a 35kgkg(-1) CO2/SCG ratio. Cupriavidus necator DSM 428 was cultivated in 2L bioreactor using extracted SCG oil as sole carbon source for production of polyhydroxyalkanoates. The culture reached a cell dry weight of 16.7gL(-1) with a polymer content of 78.4% (w/w). The volumetric polymer productivity and oil yield were 4.7gL(-1)day(-1) and 0.77gg(-1), respectively. The polymer produced was a homopolymer of 3-hydroxybutyrate with an average molecular weight of 2.34×10(5) and a polydispersity index of 1.2. The polymer exhibited brittle behaviour, with very low elongation at break (1.3%), tensile strength at break of 16MPa and Young's Modulus of 1.0GPa. Results show that SCG can be a bioresource for polyhydroxyalkanoates production with interesting properties. Copyright © 2014 Elsevier Ltd. All rights reserved.

Improved lignin pyrolysis for phenolics production in a bubbling bed reactor--Effect of bed materials.

PubMed

Li, Dongbing; Briens, Cedric; Berruti, Franco

2015-01-01

Lignin pyrolysis was studied in a bubbling fluidized bed reactor equipped with a fractional condensation train, using nitrogen as the fluidization gas. The effect of different bed materials (silica sand, lignin char, activated lignin char, birch bark char, and foamed glass beads) on bio-oil yield and quality was investigated for a pyrolysis temperature of 550 °C. Results how that a bed of activated lignin char is preferable to the commonly used silica sand: pyrolysis of Kraft lignin with a bed of activated lignin char not only provides a pure char product, but also a higher dry bio-oil yield (with a relative increase of 43%), lower pyrolytic water production, and better bio-oil quality. The bio-oil obtained from Kraft lignin pyrolysis with a bed of activated lignin char has a lower average molecular weight, less tar, more phenolics, and less acidity than when sand is used as bed material. Copyright © 2015 Elsevier Ltd. All rights reserved.

Optimization of the extraction of the p-menthadienol isomers and aristolone contained in the essential oil from Elyonurus hensii using a 23 full factorial design.

PubMed

Loumouamou, Aubin Nestor; Bikindou, Kévin; Bitemou, Ernest; Chalard, Pierre; Silou, Thomas; Figueredo, Gilles

2017-05-01

The aim of this study was to optimize the extraction of p -menthadienol isomers and aristolone from the essential oil of Elyonurus hensii by hydrodistillation. The study of the seasonal variation in the chemical composition has shown that the plant material has been subject to a natural selection regarding the biosynthesis of the p -menthadienol isomers: during periods of water stress, the extracts are rich in cis and trans-p -mentha-1(7),8-dien-2-ol and poor in cis and trans-p -mentha-2,8-dien-1-ol. Regarding the modeling, eight experiments were carried out by considering three easily interpretable factors (the extraction duration, the residual water content and the state of the division of the plant material). The average yield was 1.33% for the aerial part and 0.74% for the roots. The residual water content is the most important factor, which significantly influences the average yield of the essential oil and the content of the major constituents. Regarding the aerial part, a low residual water content of the plant material varies the essential oil yield (from 0.40% to 2.11%) and the content of cis and trans - p -mentha-2.8-dien-1-ol (from 15.87% to 23.24%). At the root level, the samples that have a very low residual water content provide extracts richer in aristolone. The combined effects of the extraction duration, the state of division, and the residual water content influence greatly the extraction of aristolone (from 36.68% to 54.55%). However, these interactions are more complex and difficult to assess.

Recent Genetic Gains in Nitrogen Use Efficiency in Oilseed Rape

PubMed Central

Stahl, Andreas; Pfeifer, Mara; Frisch, Matthias; Wittkop, Benjamin; Snowdon, Rod J.

2017-01-01

Nitrogen is essential for plant growth, and N fertilization allows farmers to obtain high yields and produce sufficient agricultural commodities. On the other hand, nitrogen losses potentially cause adverse effects to ecosystems and to human health. Increasing nitrogen use efficiency (NUE) is vital to solve the conflict between productivity, to secure the demand of a growing world population, and the protection of the environment. To ensure this, genetic improvement is considered to be a paramount aspect toward ecofriendly crop production. Winter oilseed rape (Brassica napus L.) is the second most important oilseed crop in the world and is cultivated in many regions across the temperate zones. To our knowledge, this study reports the most comprehensive field-based data generated to date for an empirical evaluation of genetic improvement in winter oilseed rape varieties under two divergent nitrogen fertilization levels (NFLs). A collection of 30 elite varieties registered between 1989 and 2014, including hybrids and open pollinated varieties, was tested in a 2-year experiment in 10 environments across Germany for changes in seed yield and seed quality traits. Furthermore, NUE was calculated. We observed a highly significant genetics-driven increase in seed yield per-se and, thus, increased NUE at both NFLs. On average, seed yield from modern open-pollinated varieties and modern hybrids was higher than from old open-pollinated varieties and old hybrids. The annual yield progress across all tested varieties was ~35 kg ha−1 year−1 at low nitrogen and 45 kg ha−1 year−1 under high nitrogen fertilization. Furthermore, in modern varieties an increased oil concentration and decreased protein concentration was observed. Despite, the significant effects of nitrogen fertilization, a surprisingly low average seed yield gap of 180 kg N ha−1 was noted between high and low nitrogen fertilization. Due to contrary effects of N fertilization on seed yield per-se and seed oil concentration an oil yield of 2.04 t ha−1 was measured at both N levels. Collectively, the data reveal that genetic improvement through modern breeding techniques in conjunction with reduced N fertilizer inputs has a tremendous potential to increase NUE of oilseed rape. PMID:28638399

Assessment of In-Place Oil Shale Resources of the Green River Formation, Piceance Basin, Western Colorado

USGS Publications Warehouse

Johnson, Ronald C.; Mercier, Tracey J.; Brownfield, Michael E.; Pantea, Michael P.; Self, Jesse G.

2009-01-01

The U.S. Geological Survey (USGS) recently completed a reassessment of in-place oil shale resources, regardless of richness, in the Eocene Green River Formation in the Piceance Basin, western Colorado. A considerable amount of oil-yield data has been collected after previous in-place assessments were published, and these data were incorporated into this new assessment. About twice as many oil-yield data points were used, and several additional oil shale intervals were included that were not assessed previously for lack of data. Oil yields are measured using the Fischer assay method. The Fischer assay method is a standardized laboratory test for determining the oil yield from oil shale that has been almost universally used to determine oil yields for Green River Formation oil shales. Fischer assay does not necessarily measure the maximum amount of oil that an oil shale can produce, and there are retorting methods that yield more than the Fischer assay yield. However, the oil yields achieved by other technologies are typically reported as a percentage of the Fischer assay oil yield, and thus Fischer assay is still considered the standard by which other methods are compared.

Method for extracting metals from aqueous waste streams for long term storage

DOEpatents

Chaiko, D.J.

1995-03-07

A liquid-liquid extraction method for removing metals and hydrous metal colloids from waste streams is provided wherein said waste streams are contacted with a solvent system containing a water-in-oil microemulsion wherein the inverted micelles contain the extracted metal. A silicon alkoxide, either alone or in combination with other metal alkoxide compounds is added to the water-in-oil microemulsion, thereby allowing encapsulation of the extracted metal within a silicon oxide network. Lastly, the now-encapsulated metal is precipitated from the water-in-oil microemulsion phase to yield aggregates of metal-silicate particles having average individual particle sizes of approximately 40 nanometers. 2 figs.

Method for extracting metals from aqueous waste streams for long term storage

DOEpatents

Chaiko, D.J.

1993-01-01

A liquid-liquid extraction method for removing metals and hydrous metal colloids from waste streams is provided wherein said waste streams are contacted with a solvent system containing a water-in-oil microemulsion wherein the inverted micelles contain the extracted metal. A silicon alkoxide, either alone or in combination with other metal alkoxide compounds is added to the water-in-oil microemulsion, thereby allowing encapsulation of the extracted metal within a silicon oxide network. Lastly, the now-encapsulated metal is precipitated from the water-in-oil microemulsion phase to yield aggregates of metal-silicate particles having average. individual particle sizes of approximately 40 manometers.

Method for extracting metals from aqueous waste streams for long term storage

DOEpatents

Chaiko, David J.

1995-01-01

A liquid--liquid extraction method for removing metals and hydrous metal colloids from waste streams is provided wherein said waste streams are contacted with a solvent system containing a water-in-oil microemulsion wherein the inverted micelles contain the extracted metal. A silicon alkoxide, either alone or in combination with other metal alkoxide compounds is added to the water-in-oil microemulsion, thereby allowing encapsulation of the extracted metal within a silicon oxide network. Lastly, the now-encapsulated metal is precipitated from the water-in-oil microemulsion phase to yield aggregates of metal-silicate particles having average individual particle sizes of approximately 40 nanometers.

Histograms showing variations in oil yield, water yield, and specific gravity of oil from Fischer assay analyses of oil-shale drill cores and cuttings from the Piceance Basin, northwestern Colorado

USGS Publications Warehouse

Dietrich, John D.; Brownfield, Michael E.; Johnson, Ronald C.; Mercier, Tracey J.

2014-01-01

Recent studies indicate that the Piceance Basin in northwestern Colorado contains over 1.5 trillion barrels of oil in place, making the basin the largest known oil-shale deposit in the world. Previously published histograms display oil-yield variations with depth and widely correlate rich and lean oil-shale beds and zones throughout the basin. Histograms in this report display oil-yield data plotted alongside either water-yield or oil specific-gravity data. Fischer assay analyses of core and cutting samples collected from exploration drill holes penetrating the Eocene Green River Formation in the Piceance Basin can aid in determining the origins of those deposits, as well as estimating the amount of organic matter, halite, nahcolite, and water-bearing minerals. This report focuses only on the oil yield plotted against water yield and oil specific gravity.

Processing Of Neem And Jatropha Methyl Esters -Alternative Fuels From Vegetable Oil

NASA Astrophysics Data System (ADS)

Ramasubramanian, S.; Manavalan, S.; Gnanavel, C.; Balakrishnan, G.

2017-03-01

Biodiesel is an alternative fuel for diesel engine. The methyl esters of vegetable oils, known as biodiesel are becoming increasingly popular because of their low environmental impact and potential as a green alternative fuel for diesel engine. This paper deals with the manufacturing process of Biodiesel from jatropha and neem oil. Biodiesel was prepared from neem oil and jatropha oil, the transestrified having kinematic viscosity of 3 & 2.6 centistokes, methanol ratio is 6:1 & 5.1respectively. The secondary solution is preheated at 65 C & 60 C and reaction temperature is maintained at 60C & 55 C and reaction time is 60 minutes approximately with NaOH catalyst and low viscosity oil is allowed to settle 24 hours. The average yield of neem and jatropha methyl esters was about 85%. These methyl esters shows excellent alternative under optimum condition for fossil fuels.

Anti-Fungal activity of essential oil from Baeckea frutescens L against Pleuratus ostreatus

NASA Astrophysics Data System (ADS)

Jemi, Renhart; Barus, Ade Irma; Nuwa, Sarinah, Luhan, Gimson

2017-11-01

Ujung Atap is an herb that have distinctive odor on its leaves. The plant's essential oil contains bioactive compounds but has not been investigated its anti-fungal activity against Pleurotus ostreatus. Essential oil from Ujung Atap leaves is one environmentally friendly natural preservative. This study consisted of distillation Ujung Atap leaves with boiled method, determining the number of acid, essential oil ester, and anti-fungal activity against Pleurotus ostreatus. Analysis of the data to calculate anti-fungal activity used probit analysis method to determine the IC50. Results for the distillation of leaves Ujung Atap produce essential oil yield of 0.071% and the average yield of the acid number and the ester of essential oils Ujung Atap leaves are 5.24 and 12.15. Anti-fungal activity Pleurotus ostreatus at a concentration of 1000 µg/mL, 100 µg/mL, 75 µg/mL, 50 µg/mL and 100 µg/mL BA defunct or fungi was declared dead, while at a concentration of 25 µg/mL, 10 µg/mL and 5 µg/mL still occur inhibitory processes. Results obtained probit analysis method IC50 of 35.48 mg/mL; means the essential oil of Ujung Atap leaf can inhibit fungal growth by 50 percent to 35.48 µg/mL concentration.

Oil point and mechanical behaviour of oil palm kernels in linear compression

NASA Astrophysics Data System (ADS)

Kabutey, Abraham; Herak, David; Choteborsky, Rostislav; Mizera, Čestmír; Sigalingging, Riswanti; Akangbe, Olaosebikan Layi

2017-07-01

The study described the oil point and mechanical properties of roasted and unroasted bulk oil palm kernels under compression loading. The literature information available is very limited. A universal compression testing machine and vessel diameter of 60 mm with a plunger were used by applying maximum force of 100 kN and speed ranging from 5 to 25 mm min-1. The initial pressing height of the bulk kernels was measured at 40 mm. The oil point was determined by a litmus test for each deformation level of 5, 10, 15, 20, and 25 mm at a minimum speed of 5 mmmin-1. The measured parameters were the deformation, deformation energy, oil yield, oil point strain and oil point pressure. Clearly, the roasted bulk kernels required less deformation energy compared to the unroasted kernels for recovering the kernel oil. However, both kernels were not permanently deformed. The average oil point strain was determined at 0.57. The study is an essential contribution to pursuing innovative methods for processing palm kernel oil in rural areas of developing countries.

Linear least-squares method for global luminescent oil film skin friction field analysis

NASA Astrophysics Data System (ADS)

Lee, Taekjin; Nonomura, Taku; Asai, Keisuke; Liu, Tianshu

2018-06-01

A data analysis method based on the linear least-squares (LLS) method was developed for the extraction of high-resolution skin friction fields from global luminescent oil film (GLOF) visualization images of a surface in an aerodynamic flow. In this method, the oil film thickness distribution and its spatiotemporal development are measured by detecting the luminescence intensity of the thin oil film. From the resulting set of GLOF images, the thin oil film equation is solved to obtain an ensemble-averaged (steady) skin friction field as an inverse problem. In this paper, the formulation of a discrete linear system of equations for the LLS method is described, and an error analysis is given to identify the main error sources and the relevant parameters. Simulations were conducted to evaluate the accuracy of the LLS method and the effects of the image patterns, image noise, and sample numbers on the results in comparison with the previous snapshot-solution-averaging (SSA) method. An experimental case is shown to enable the comparison of the results obtained using conventional oil flow visualization and those obtained using both the LLS and SSA methods. The overall results show that the LLS method is more reliable than the SSA method and the LLS method can yield a more detailed skin friction topology in an objective way.

Optimization of the omega-3 extraction as a functional food from flaxseed.

PubMed

Hassan-Zadeh, A; Sahari, M A; Barzegar, M

2008-09-01

The fatty acid content, total lipid, refractive index, peroxide, iodine, acid and saponification values of Iranian linseed oil (Linum usitatissimum) were studied. For optimization of extraction conditions, this oil was extracted by solvents (petroleum benzene and methanol-water-petroleum benzene) in 1:2, 1:3 and 1:4 ratios at 2, 5 and 8 h. Then its fatty acid content, omega-3 content and extraction yield were determined. According to the statistical analysis, petroleum benzene in a ratio of 1:3 at 5 h was chosen for the higher fatty acid, extraction yield, and economical feasibility. For preservation of omega-3 ingredients, oil with specified characters containing 46.8% omega-3 was kept under a nitrogen atmosphere at -30 degrees C during 0, 7, 30, 60 and 90 days and its peroxide value was determined. Statistical analysis showed a significant difference in the average amount of peroxide value only on the first 7 days of storage, and its increase (8.30%) conformed to the international standard.

[Selective breeding research on new cultivar "ChuanPeng 1" of Curcuma phaeocaulis].

PubMed

Xia, Qin; Yang, Zhao-wu; Li, Min; Xia, Dong-mei

2014-11-01

To breed a new good cultivar of Curcuma phaeocaulis. Three rounds of selection were systematically made for screening the new cultivar using biological technology. Firstly, individual plant selection. Promising individual plant selection was made based on the thousands of Curcuma phaeocaulis resources collected from all over the country. Secondly, strain selection. The promising strain was selected continually from the superior individuals on basis of specificities such as biological characteristics, yield, content of volatile oil and resistance. Thirdly, strain comparison test. The superior strain was selected through strain trial and regional trial. For the new cultivar "ChuanPeng 1" , its main rhizome was in ovoid or spindle shape, and it was fleshy and corpulent. Average amount of rhizome was 4, with 6. 27 cm in length and 3. 37 cm in diameter, while the secondary roots were cylindrical-like, with a yellow green cross section as the main rhizome. Slender root ends were inflated into the fleshy spindle shape, average amount of whose was 25, 3. 20 cm in length and 1. 33 cm in diameter with a yellow green or near white cross section. Average yield of rhizome is 5 314. 5 kg/hm2, while the root is 1 942. 5 kg/hm2, which was 32. 9% and 22. 7% higher than the local main cultivar respectively. The average extract content of rhizome was 15. 41% and content of volatile oil was 2. 82%, which was 15. 60% and 9. 30% higher than the local cultivar respectively. " ChuanPeng 1" has good stability and strong adaptability with high yield and superior internal quality. It can be cultivated and promoted in Jinma River Valley,Sichuan.

The influence of diets containing either conventional corn, conventional corn with choice white grease, high oil corn, or high oil high oleic corn on belly/bacon quality.

PubMed

Rentfrow, G; Sauber, T E; Allee, G L; Berg, E P

2003-08-01

The objectives of this study were to evaluate diets possessing different fatty acid profiles (as influenced by corn type) with regard to fatty acid profile and firmness of pork bellies. Crossbred barrows (n=196) were fed one of four corn-based diets consisting of conventional corn (CONV), CONV with choice white grease (CWG), high oil corn (HOC), or high oleic, high oil corn (HOHOC). Following 98 days on test, two animals representing the average pen weight (118 kg) were selected for harvest (n=56). A 50-g fat sample was removed from each belly for fatty acid profile analysis. Lateral and vertical flex tests were performed to determine belly firmness. Bellies were pumped and cooked according to a commercial protocol. Total saturated fatty acids increased (P<0.001) and total unsaturated fatty acids decreased (P<0.05) when CWG was added to the CONV diet or when HOC or HOHOC were substituted for CONV corn. Pigs fed CONV corn had firmer bellies, while those fed HOC were softer. No differences were observed across treatment for percentage pump retention, smokehouse yield, or slicing yield (P>0.05). Based on the results of this study, corn type influences fatty acid profile, and belly firmness, but does not affect pump retention, or slicing yields.

Measurement of νμ-induced charged-current neutral pion production cross sections on mineral oil at Eν∈0.5-2.0GeV

NASA Astrophysics Data System (ADS)

Aguilar-Arevalo, A. A.; Anderson, C. E.; Bazarko, A. O.; Brice, S. J.; Brown, B. C.; Bugel, L.; Cao, J.; Coney, L.; Conrad, J. M.; Cox, D. C.; Curioni, A.; Dharmapalan, R.; Djurcic, Z.; Finley, D. A.; Fleming, B. T.; Ford, R.; Garcia, F. G.; Garvey, G. T.; Grange, J.; Green, C.; Green, J. A.; Hart, T. L.; Hawker, E.; Imlay, R.; Johnson, R. A.; Karagiorgi, G.; Kasper, P.; Katori, T.; Kobilarcik, T.; Kourbanis, I.; Koutsoliotas, S.; Laird, E. M.; Linden, S. K.; Link, J. M.; Liu, Y.; Liu, Y.; Louis, W. C.; Mahn, K. B. M.; Marsh, W.; Mauger, C.; McGary, V. T.; McGregor, G.; Metcalf, W.; Meyers, P. D.; Mills, F.; Mills, G. B.; Monroe, J.; Moore, C. D.; Mousseau, J.; Nelson, R. H.; Nienaber, P.; Nowak, J. A.; Osmanov, B.; Ouedraogo, S.; Patterson, R. B.; Pavlovic, Z.; Perevalov, D.; Polly, C. C.; Prebys, E.; Raaf, J. L.; Ray, H.; Roe, B. P.; Russell, A. D.; Sandberg, V.; Schirato, R.; Schmitz, D.; Shaevitz, M. H.; Shoemaker, F. C.; Smith, D.; Soderberg, M.; Sorel, M.; Spentzouris, P.; Spitz, J.; Stancu, I.; Stefanski, R. J.; Sung, M.; Tanaka, H. A.; Tayloe, R.; Tzanov, M.; van de Water, R. G.; Wascko, M. O.; White, D. H.; Wilking, M. J.; Yang, H. J.; Zeller, G. P.; Zimmerman, E. D.

2011-03-01

Using a custom 3-Čerenkov ring fitter, we report cross sections for νμ-induced charged-current single π0 production on mineral oil (CH2) from a sample of 5810 candidate events with 57% signal purity over an energy range of 0.5-2.0 GeV. This includes measurements of the absolute total cross section as a function of neutrino energy, and flux-averaged differential cross sections measured in terms of Q2, μ- kinematics, and π0 kinematics. The sample yields a flux-averaged total cross section of (9.2±0.3stat±1.5syst)×10-39cm2/CH2 at mean neutrino energy of 0.965 GeV.

Optimization of supercritical carbon dioxide extraction of essential oil from Dracocephalum kotschyi Boiss: An endangered medicinal plant in Iran.

PubMed

Nejad-Sadeghi, Masoud; Taji, Saeed; Goodarznia, Iraj

2015-11-27

Extraction of the essential oil from a medicinal plant called Dracocephalum kotschyi Boiss was performed by green technology of supercritical carbon dioxide (SC-CO2) extraction. A Taguchi orthogonal array design with an OA16 (4(5)) matrix was used to evaluate the effects of five extraction variables: pressure of 150-310bar, temperature of 40-60°C, average particle size of 250-1000μm, CO2 flow rate of 2-10ml/s and dynamic extraction time of 30-100min. The optimal conditions to obtain the maximum extraction yield were at 240bar, 60°C, 500μm, 10ml/s and 100min. The extraction yield under the above conditions was 2.72% (w/w) which is more than two times the maximum extraction yield that has been reported for this plant in the literature using traditional extraction techniques. Results from analysis of variance (ANOVA) indicated that the CO2 flow rate and the extraction time were the most significant factors on the extraction yield by percentage contribution of 44.27 and 28.86, respectively. Finally, the chemical composition of the essential oil was evaluated by using gas chromatography-mass spectroscopy (GC-MS). Citral, p-mentha-1,3,8-triene, D-3-carene and methyl geranate were the major components identified. Copyright © 2015. Published by Elsevier B.V.

Genetic progress in sunflower crop in Rio Grande do Sul State, Brazil.

PubMed

Follmann, D N; Cargnelutti Filho, A; Lorentz, L H; Boligon, A A; Caraffa, M; Wartha, C A

2017-04-13

The sunflower has adaptability to growing regions with different climatic and soil characteristics, showing drought tolerance and high-quality oil production. The State of Rio Grande do Sul is the third largest sunflower producer in Brazil, with research related to the sunflower breeding initiated after the decade of 1950. The aim of this study was to evaluate the genetic progress for grain yield, oil content, and oil yield of sunflower (Helianthus annuus L.) in the State of Rio Grande do Sul. Data of grain yield, oil content, and oil yield obtained from 58 sunflower cultivar yield trials in 19 municipalities in Rio Grande do Sul during the period from 2005 to 2014 were used. Genetic progress was studied according to the methodology proposed by Vencovsky and data from sunflower cultivar yield trials were used. Annual genetic progress of sunflower during the period of 10 years (2005-2014) was 132.46 kg⋅ha -1 ⋅year -1 for grain yield, -0.17%/year for oil content, and 48.11 kg⋅ha -1 ⋅year -1 for oil yield. The sunflower-breeding programs in the State of Rio Grande do Sul were efficient for the traits grain yield and oil yield and presented no efficiency for oil content.

Concurrent elevation of CO2, O3 and temperature severely affects oil quality and quantity in rapeseed

PubMed Central

Namazkar, Shahla; Stockmarr, Anders; Frenck, Georg; Egsgaard, Helge; Terkelsen, Thilde; Mikkelsen, Teis; Ingvordsen, Cathrine Heinz; Jørgensen, Rikke Bagger

2016-01-01

Plant oil is an essential dietary and bio-energy resource. Despite this, the effects of climate change on plant oil quality remain to be elucidated. The present study is the first to show changes in oil quality and quantity of four rapeseed cultivars in climate scenarios with elevated [CO2], [O3] and temperature (T) combined and as single factors. The combination of environmental factors resembled IPCC’s ‘business as usual’ emission scenario predicted for late this century. Generally, the climate scenarios reduced the average amounts of the six fatty acids (FAs) analysed, though in some treatments single FAs remained unchanged or even increased. Most reduced was the FA essential for human nutrition, C18:3-ω3, which decreased by 39% and 45% in the combined scenarios with elevated [CO2]+T+[O3] and [CO2]+T, respectively. Average oil content decreased 3–17%. When [CO2] and T were elevated concurrently, the seed biomass was reduced by half, doubling the losses in FAs and oil content. This corresponded to a 58% reduction in the oil yield per hectare, and C18:3-ω3 decreased by 77%. Furthermore, the polyunsaturated FAs were significantly decreased. The results indicate undesirable consequences for production and health benefits of rapeseed oil with future climate change. The results also showed strong interactive effects of CO2, T and O3 on oil quality, demonstrating why prediction of climate effects requires experiments with combined factors and should not be based on extrapolation from single factor experiments. PMID:27222513

Biodiesel production from waste cooking oil using copper doped zinc oxide nanocomposite as heterogeneous catalyst.

PubMed

Gurunathan, Baskar; Ravi, Aiswarya

2015-01-01

A novel CZO nanocomposite was synthesized and used as heterogeneous catalyst for transesterification of waste cooking oil into biodiesel using methanol as acyl acceptor. The synthesized CZO nanocomposite was characterized in FESEM with an average size of 80 nm as nanorods. The XRD patterns indicated the substitution of ZnO in the hexagonal lattice of Cu nanoparticles. The 12% (w/w) nanocatalyst concentration, 1:8 (v:v) O:M ratio, 55 °C temperature and 50 min of reaction time were found as optimum for maximum biodiesel yield of 97.71% (w/w). Hence, the use of CZO nanocomposite can be used as heterogeneous catalyst for biodiesel production from waste cooking oil. Copyright © 2015 Elsevier Ltd. All rights reserved.

Differential gene expression at different stages of mesocarp development in high- and low-yielding oil palm.

PubMed

Wong, Yick Ching; Teh, Huey Fang; Mebus, Katharina; Ooi, Tony Eng Keong; Kwong, Qi Bin; Koo, Ka Loo; Ong, Chuang Kee; Mayes, Sean; Chew, Fook Tim; Appleton, David R; Kulaveerasingam, Harikrishna

2017-06-21

The oil yield trait of oil palm is expected to involve multiple genes, environmental influences and interactions. Many of the underlying mechanisms that contribute to oil yield are still poorly understood. In this study, we used a microarray approach to study the gene expression profiles of mesocarp tissue at different developmental stages, comparing genetically related high- and low- oil yielding palms to identify genes that contributed to the higher oil-yielding palm and might contribute to the wider genetic improvement of oil palm breeding populations. A total of 3412 (2001 annotated) gene candidates were found to be significantly differentially expressed between high- and low-yielding palms at at least one of the different stages of mesocarp development evaluated. Gene Ontologies (GO) enrichment analysis identified 28 significantly enriched GO terms, including regulation of transcription, fatty acid biosynthesis and metabolic processes. These differentially expressed genes comprise several transcription factors, such as, bHLH, Dof zinc finger proteins and MADS box proteins. Several genes involved in glycolysis, TCA, and fatty acid biosynthesis pathways were also found up-regulated in high-yielding oil palm, among them; pyruvate dehydrogenase E1 component Subunit Beta (PDH), ATP-citrate lyase, β- ketoacyl-ACP synthases I (KAS I), β- ketoacyl-ACP synthases III (KAS III) and ketoacyl-ACP reductase (KAR). Sucrose metabolism-related genes such as Invertase, Sucrose Synthase 2 and Sucrose Phosphatase 2 were found to be down-regulated in high-yielding oil palms, compared to the lower yield palms. Our findings indicate that a higher carbon flux (channeled through down-regulation of the Sucrose Synthase 2 pathway) was being utilized by up-regulated genes involved in glycolysis, TCA and fatty acid biosynthesis leading to enhanced oil production in the high-yielding oil palm. These findings are an important stepping stone to understand the processes that lead to production of high-yielding oil palms and have implications for breeding to maximize oil production.

Measurement of ν μ-induced charged-current neutral pion production cross sections on mineral oil at E vϵ0.5–2.0 GeV

DOE PAGES

Aguilar-Arevalo, A. A.; Anderson, C. E.; Bazarko, A. O.; ...

2011-03-23

Using a custom 3-Cerenkov ring fitter, we report cross sections for ν μ-induced charged-current single π⁰ production on mineral oil (CH₂) from a sample of 5810 candidate events with 57% signal purity over an energy range of 0.5–2.0 GeV. This includes measurements of the absolute total cross section as a function of neutrino energy, and flux-averaged differential cross sections measured in terms of Q², μ⁻ kinematics, and π⁰ kinematics. The sample yields a flux-averaged total cross section of (9.2±0.3 stat±1.5 syst)×10⁻³⁹ cm²/CH² at mean neutrino energy of 0.965 GeV.

Heterotic patterns in rapeseed (Brassica napus L.): I. Crosses between spring and Chinese semi-winter lines.

PubMed

Qian, W; Sass, O; Meng, J; Li, M; Frauen, M; Jung, C

2007-06-01

Chinese semi-winter rapeseed is genetically diverse from Canadian and European spring rapeseed. This study was conducted to evaluate the potential of semi-winter rapeseed for spring rapeseed hybrid breeding, to assess the genetic effects involved, and to estimate the correlation of parental genetic distance (GD) with hybrid performance, heterosis, general combining ability (GCA) and specific combining ability (SCA) in crosses between spring and semi-winter rapeseed lines. Four spring male sterile lines from Germany and Canada as testers were crossed with 13 Chinese semi-winter rapeseed lines to develop 52 hybrids, which were evaluated together with their parents and commercial hybrids for seed yield and oil content in three sets of field trials with 8 environments in Canada and Europe. The Chinese parental lines were not adapted to local environmental conditions as demonstrated by poor seed yields per se. However, the hybrids between the Chinese parents and the adapted spring rapeseed lines exhibited high heterosis for seed yield. The average mid-parent heterosis was 15% and ca. 50% of the hybrids were superior to the respective hybrid control across three sets of field trials. Additive gene effects mainly contributed to hybrid performance since the mean squares of GCA were higher as compared to SCA. The correlation between parental GD and hybrid performance and heterosis was found to be low whereas the correlation between GCA((f + m)) and hybrid performance was high and significant in each set of field trials, with an average of r = 0.87 for seed yield and r = 0.89 for oil content, indicating that hybrid performance can be predicted by GCA((f + m)). These results demonstrate that Chinese semi-winter rapeseed germplasm has a great potential to increase seed yield in spring rapeseed hybrid breeding programs in Canada and Europe.

Pulse-echo probe of rock permeability near oil wells

NASA Technical Reports Server (NTRS)

Narasimhan, K. Y.; Parthasarathy, S. P.

1978-01-01

Processing method involves sequential insonifications of borehole wall at number of different frequencies. Return signals are normalized in amplitude, and root-mean-square (rms) value of each signal is determined. Values can be processed to yield information on size and number density of microfractures at various depths in rock matrix by using averaging methods developed for pulse-echo technique.

Liquid smoke characteristics from the pyrolysis of oil palm fronds

NASA Astrophysics Data System (ADS)

Maulina, S.; Silia, F.

2018-02-01

This study was conducted as means to characterize the pyrolysis of oil palm fronds into more economical products. In particular, this study was focused on pyrolysis of oil palm fronds, which could generate products such as liquid smoke, tar and char. Four characteristics of liquid smoke were examined in this study, namely the yield of liquid smoke, phenolic content, total acid content and pH. These characteristics were examined in a temperature of 150 °C, 200 °C and 250 °C with processing time of 60 minutes, 90 minutes and 120 minutes. This study revealed that the highest yield of liquid smoke was equal to 43.47% at a temperature of 150 °C for approximately 2 hours, while the highest level of phenolic was obtained at a temperature of 250 °C for approximately 1 hour. Moreover, the highest total acid content obtained was 11.23% at a temperature of 150 °C with a time of 1 hour. In addition, all operating conditions has produced liquid smoke with an average pH value of 3.

Compatibility between weak gel and microorganisms in weak gel-assisted microbial enhanced oil recovery.

PubMed

Qi, Yi-Bin; Zheng, Cheng-Gang; Lv, Cheng-Yuan; Lun, Zeng-Min; Ma, Tao

2018-03-20

To investigate weak gel-assisted microbial flooding in Block Wang Long Zhuang in the Jiangsu Oilfield, the compatibility of weak gel and microbe was evaluated using laboratory experiments. Bacillus sp. W5 was isolated from the formation water in Block Wang Long Zhuang. The rate of oil degradation reached 178 mg/day, and the rate of viscosity reduction reached 75.3%. Strain W5 could produce lipopeptide with a yield of 1254 mg/L. Emulsified crude oil was dispersed in the microbial degradation system, and the average diameter of the emulsified oil particles was 18.54 μm. Bacillus sp. W5 did not affect the rheological properties of the weak gel, and the presence of the weak gel did not significantly affect bacterial reproduction (as indicated by an unchanged microbial biomass), emulsification (surface tension is 35.56 mN/m and average oil particles size is 21.38 μm), oil degradation (162 mg/day) and oil viscosity reduction (72.7%). Core-flooding experiments indicated oil recovery of 23.6% when both weak gel and Bacillus sp. W5 were injected into the system, 14.76% when only the weak gel was injected, and 9.78% with strain W5 was injected without the weak gel. The results demonstrate good compatibility between strains W5 and the weak gel and highlight the application potential of weak gel-assisted microbial flooding. Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Up-regulation of an N-terminal truncated 3-hydroxy-3-methylglutaryl CoA reductase enhances production of essential oils and sterols in transgenic Lavandula latifolia.

PubMed

Muñoz-Bertomeu, Jesús; Sales, Ester; Ros, Roc; Arrillaga, Isabel; Segura, Juan

2007-11-01

Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lavender plants expressing the Arabidopsis thaliana HMG1 cDNA, encoding the catalytic domain of 3-hydroxy-3-methylglutaryl CoA reductase (HMGR1S), a key enzyme of the mevalonic acid (MVA) pathway. Transgenic T0 plants accumulated significantly more essential oil constituents as compared to controls (up to 2.1- and 1.8-fold in leaves and flowers, respectively). Enhanced expression of HMGR1S also increased the amount of the end-product sterols, beta-sitosterol and stigmasterol (average differences of 1.8- and 1.9-fold, respectively), but did not affect the accumulation of carotenoids or chlorophylls. We also analysed T1 plants derived from self-pollinated seeds of T0 lines that flowered after growing for 2 years in the greenhouse. The increased levels of essential oil and sterols observed in the transgenic T0 plants were maintained in the progeny that inherited the HMG1 transgene. Our results demonstrate that genetic manipulation of the MVA pathway increases essential oil yield in spike lavender, suggesting a contribution for this cytosolic pathway to monoterpene and sesquiterpene biosynthesis in leaves and flowers of the species.

Antimicrobial and seasonal evaluation of the carvacrol-chemotype oil from Lippia origanoides kunth.

PubMed

Sarrazin, Sandra Layse F; da Silva, Leomara Andrade; de Assunção, Ana Paula F; Oliveira, Ricardo B; Calao, Victor Y P; da Silva, Rodrigo; Stashenko, Elena E; Maia, José Guilherme S; Mourão, Rosa Helena V

2015-01-23

This study evaluated the influence of seasonal variation on the yield and composition of essential oil of Lippia origanoides occurring in the Middle Rio Amazonas, Brazil, and the impact on its antimicrobial potential. The average oil yield was 1.7% ± 0.2% in the rainy season and 1.6% ± 0.3% in the dry season. Some correlations with climatic parameters were observed. The major components were carvacrol (rainy, 43.5% ± 1.9%; dry, 41.4% ± 2.04%), thymol (rainy, 10.7% ± 1.1%; dry, 10.6% ± 0.9%), p-cymene (rainy, 9.8% ± 0.7%; dry, 10.0% ± 1.4%) and p-methoxythymol (rainy, 9.6% ± 0.8%; dry, 10.4% ± 1.4%). It was found that the antibacterial activity of L. origanoides against Staphylococcus aureus and Escherichia coli was little influenced by the changes in oil composition due to seasonal variation. Against S. aureus, the oil Minimum Inhibitory Concentration (MIC) value was 1.25 μL/mL over ten months. Against E. coli, the oil MIC values ranged from 0.15 μL/mL to 0.31 μL/mL in different months of the year. The Minimum Bactericidal Concentration (MBC) value was 2.5 μL/mL against S. aureus and 1.25 μL/mL against E. coli. The results suggest that the antimicrobial activity identified in the oil remain unchanged for the full year, allowing its medicinal use without any risk of loss or absence of the active principles of the plant.

Concurrent elevation of CO2, O3 and temperature severely affects oil quality and quantity in rapeseed.

PubMed

Namazkar, Shahla; Stockmarr, Anders; Frenck, Georg; Egsgaard, Helge; Terkelsen, Thilde; Mikkelsen, Teis; Ingvordsen, Cathrine Heinz; Jørgensen, Rikke Bagger

2016-07-01

Plant oil is an essential dietary and bio-energy resource. Despite this, the effects of climate change on plant oil quality remain to be elucidated. The present study is the first to show changes in oil quality and quantity of four rapeseed cultivars in climate scenarios with elevated [CO2], [O3] and temperature (T) combined and as single factors. The combination of environmental factors resembled IPCC's 'business as usual' emission scenario predicted for late this century. Generally, the climate scenarios reduced the average amounts of the six fatty acids (FAs) analysed, though in some treatments single FAs remained unchanged or even increased. Most reduced was the FA essential for human nutrition, C18:3-ω3, which decreased by 39% and 45% in the combined scenarios with elevated [CO2]+T+[O3] and [CO2]+T, respectively. Average oil content decreased 3-17%. When [CO2] and T were elevated concurrently, the seed biomass was reduced by half, doubling the losses in FAs and oil content. This corresponded to a 58% reduction in the oil yield per hectare, and C18:3-ω3 decreased by 77%. Furthermore, the polyunsaturated FAs were significantly decreased. The results indicate undesirable consequences for production and health benefits of rapeseed oil with future climate change. The results also showed strong interactive effects of CO2, T and O3 on oil quality, demonstrating why prediction of climate effects requires experiments with combined factors and should not be based on extrapolation from single factor experiments. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

[Effect of near infrared spectrum on the precision of PLS model for oil yield from oil shale].

PubMed

Wang, Zhi-Hong; Liu, Jie; Chen, Xiao-Chao; Sun, Yu-Yang; Yu, Yang; Lin, Jun

2012-10-01

It is impossible to use present measurement methods for the oil yield of oil shale to realize in-situ detection and these methods unable to meet the requirements of the oil shale resources exploration and exploitation. But in-situ oil yield analysis of oil shale can be achieved by the portable near infrared spectroscopy technique. There are different correlativities of NIR spectrum data formats and contents of sample components, and the different absorption specialities of sample components shows in different NIR spectral regions. So with the proportioning samples, the PLS modeling experiments were done by 3 formats (reflectance, absorbance and K-M function) and 4 regions of modeling spectrum, and the effect of NIR spectral format and region to the precision of PLS model for oil yield from oil shale was studied. The results show that the best data format is reflectance and the best modeling region is combination spectral range by PLS model method and proportioning samples. Therefore, the appropriate data format and the proper characteristic spectral region can increase the precision of PLS model for oil yield form oil shale.

[Effects of sowing date and planting density on the seed yield and oil content of winter oilseed rape].

PubMed

Zhang, Shu-Jie; Li, Ling; Zhang, Chun-Lei

2012-05-01

A field experiment was conducted to investigate the effects of different sowing date and planting density on the seed yield and seed oil content of winter oilseed rape (Brassica napus). Sowing date mainly affected the seed yield of branch raceme, while planting density affected the seed yields of both branch raceme and main raceme. The seed oil content was less affected by sowing date. The proportion of the seed yield of main raceme to the seed yield per plant increased with increasing planting density, and the seed oil content of main raceme was about 1% higher than that of branch raceme. Consequently, the seed oil production per plot increased significantly with increasing planting density. In the experimental region, the sowing date of winter oilseed rape should be earlier than mid-October. When sowing in late October, the seed yield would be decreased significantly. A planting density of 36-48 plants x m(-2) could improve the seed yield and oil content of winter oilseed rape.

Canola Oil in Lactating Dairy Cow Diets Reduces Milk Saturated Fatty Acids and Improves Its Omega-3 and Oleic Fatty Acid Content

PubMed Central

2016-01-01

To produce milk that is healthier for human consumption, the present study evaluated the effect of including canola oil in the diet of dairy cows on milk production and composition as well as the nutritional quality of this milk fat. Eighteen Holstein cows with an average daily milk yield of 22 (± 4) kg/d in the middle stage of lactation were used. The cows were distributed in 6 contemporary 3x3 Latin squares consisting of 3 periods and 3 treatments: control diet (without oil), 3% inclusion of canola oil in the diet and 6% inclusion of canola oil in the diet (dry matter basis). The inclusion of 6% canola oil in the diet of lactating cows linearly reduced the milk yield by 2.51 kg/d, short-chain fatty acids (FA) by 41.42%, medium chain FA by 27.32%, saturated FA by 20.24%, saturated/unsaturated FA ratio by 39.20%, omega-6/omega-3 ratio by 39.45%, and atherogenicity index by 48.36% compared with the control treatment. Moreover, with the 6% inclusion of canola oil in the diet of cows, there was an increase in the concentration of long chain FA by 45.91%, unsaturated FA by 34.08%, monounsaturated FA by 40.37%, polyunsaturated FA by 17.88%, milk concentration of omega-3 by 115%, rumenic acid (CLA) by 16.50%, oleic acid by 44.87% and h/H milk index by 94.44% compared with the control treatment. Thus, the inclusion of canola oil in the diet of lactating dairy cows makes the milk fatty acid profile nutritionally healthier for the human diet; however, the lactating performance of dairy cows is reduce. PMID:27015405

Canola Oil in Lactating Dairy Cow Diets Reduces Milk Saturated Fatty Acids and Improves Its Omega-3 and Oleic Fatty Acid Content.

PubMed

Welter, Katiéli Caroline; Martins, Cristian Marlon de Magalhães Rodrigues; de Palma, André Soligo Vizeu; Martins, Mellory Martinson; Dos Reis, Bárbara Roqueto; Schmidt, Bárbara Laís Unglaube; Saran Netto, Arlindo

2016-01-01

To produce milk that is healthier for human consumption, the present study evaluated the effect of including canola oil in the diet of dairy cows on milk production and composition as well as the nutritional quality of this milk fat. Eighteen Holstein cows with an average daily milk yield of 22 (± 4) kg/d in the middle stage of lactation were used. The cows were distributed in 6 contemporary 3x3 Latin squares consisting of 3 periods and 3 treatments: control diet (without oil), 3% inclusion of canola oil in the diet and 6% inclusion of canola oil in the diet (dry matter basis). The inclusion of 6% canola oil in the diet of lactating cows linearly reduced the milk yield by 2.51 kg/d, short-chain fatty acids (FA) by 41.42%, medium chain FA by 27.32%, saturated FA by 20.24%, saturated/unsaturated FA ratio by 39.20%, omega-6/omega-3 ratio by 39.45%, and atherogenicity index by 48.36% compared with the control treatment. Moreover, with the 6% inclusion of canola oil in the diet of cows, there was an increase in the concentration of long chain FA by 45.91%, unsaturated FA by 34.08%, monounsaturated FA by 40.37%, polyunsaturated FA by 17.88%, milk concentration of omega-3 by 115%, rumenic acid (CLA) by 16.50%, oleic acid by 44.87% and h/H milk index by 94.44% compared with the control treatment. Thus, the inclusion of canola oil in the diet of lactating dairy cows makes the milk fatty acid profile nutritionally healthier for the human diet; however, the lactating performance of dairy cows is reduce.

Distillation Time as Tool for Improved Antimalarial Activity and Differential Oil Composition of Cumin Seed Oil.

PubMed

Zheljazkov, Valtcho D; Gawde, Archana; Cantrell, Charles L; Astatkie, Tess; Schlegel, Vicki

2015-01-01

A steam distillation extraction kinetics experiment was conducted to estimate essential oil yield, composition, antimalarial, and antioxidant capacity of cumin (Cuminum cyminum L.) seed (fruits). Furthermore, regression models were developed to predict essential oil yield and composition for a given duration of the steam distillation time (DT). Ten DT durations were tested in this study: 5, 7.5, 15, 30, 60, 120, 240, 360, 480, and 600 min. Oil yields increased with an increase in the DT. Maximum oil yield (content, 2.3 g/100 seed), was achieved at 480 min; longer DT did not increase oil yields. The concentrations of the major oil constituents α-pinene (0.14-0.5% concentration range), β-pinene (3.7-10.3% range), γ-cymene (5-7.3% range), γ-terpinene (1.8-7.2% range), cumin aldehyde (50-66% range), α-terpinen-7-al (3.8-16% range), and β-terpinen-7-al (12-20% range) varied as a function of the DT. The concentrations of α-pinene, β-pinene, γ-cymene, γ-terpinene in the oil increased with the increase of the duration of the DT; α-pinene was highest in the oil obtained at 600 min DT, β-pinene and γ-terpinene reached maximum concentrations in the oil at 360 min DT; γ-cymene reached a maximum in the oil at 60 min DT, cumin aldehyde was high in the oils obtained at 5-60 min DT, and low in the oils obtained at 240-600 min DT, α-terpinen-7-al reached maximum in the oils obtained at 480 or 600 min DT, whereas β-terpinen-7-al reached a maximum concentration in the oil at 60 min DT. The yield of individual oil constituents (calculated from the oil yields and the concentration of a given compound at a particular DT) increased and reached a maximum at 480 or 600 min DT. The antimalarial activity of the cumin seed oil obtained during the 0-5 and at 5-7.5 min DT timeframes was twice higher than the antimalarial activity of the oils obtained at the other DT. This study opens the possibility for distinct marketing and utilization for these improved oils. The antioxidant capacity of the oil was highest in the oil obtained at 30 min DT and lowest in the oil from 360 min DT. The Michaelis-Menton and the Power nonlinear regression models developed in this study can be utilized to predict essential oil yield and composition of cumin seed at any given duration of DT and may also be useful to compare previous reports on cumin oil yield and composition. DT can be utilized to obtain cumin seed oil with improved antimalarial activity, improved antioxidant capacity, and with various compositions.

[Research on outlier detection methods for determination of oil yield in oil shales using near-infrared spectroscopy].

PubMed

Zhang, Huai-zhu; Lin, Jun; Zhang, Huai-Zhu

2014-06-01

In the present paper, the outlier detection methods for determination of oil yield in oil shale using near-infrared (NIR) diffuse reflection spectroscopy was studied. During the quantitative analysis with near-infrared spectroscopy, environmental change and operator error will both produce outliers. The presence of outliers will affect the overall distribution trend of samples and lead to the decrease in predictive capability. Thus, the detection of outliers are important for the construction of high-quality calibration models. The methods including principal component analysis-Mahalanobis distance (PCA-MD) and resampling by half-means (RHM) were applied to the discrimination and elimination of outliers in this work. The thresholds and confidences for MD and RHM were optimized using the performance of partial least squares (PLS) models constructed after the elimination of outliers, respectively. Compared with the model constructed with the data of full spectrum, the values of RMSEP of the models constructed with the application of PCA-MD with a threshold of a value equal to the sum of average and standard deviation of MD, RHM with the confidence level of 85%, and the combination of PCA-MD and RHM, were reduced by 48.3%, 27.5% and 44.8%, respectively. The predictive ability of the calibration model has been improved effectively.

Microbial screening test for lignite degradation. Quarterly progress report No. 4, October-December 1985

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yen, T.F.

1985-01-01

Chemical oxidation of lignite by means of hydrogen peroxide - acetic acid was executed. Methanol fractionation of the oxidized product yielded 76.4% methanol solubles, 9.3% methanol insolubles and 11.2% CO/sub 2/. Biodegradation of the methanol fraction by soil bacteria was found to be positive as demonstrated by gel permeation chromatography (GPC). The shift of the average molecular weight throughout biodegradation was estimated to be from 310 g/mole, to 243 g/mole, zero day and four weeks respectively. Biodegradation of lignitic substrates, benzene - methanol fraction (A - 1), aqueous alkaline fraction (A - 2), clean lignite residue (A - 3), rawmore » lignite, and methanol soluble fraction after oxidation, by means of oil - field soil bacteria and Polyporus versicolor ATCC 12679 was attempted. All previous mentioned lignitic substrates yielded positive results for oil-field soil bacteria, while A - 2 fraction and raw lignite yielded positive results for P. versicolor. Unidentified fungi strains, N 1, L 1, and L 2 were also tested on lignitic substrates. Mild growth was observed in these cases.« less

Changes in growth, essential oil yield and composition of geranium (Pelargonium graveolens L.) as affected by growing media.

PubMed

Rezaei Nejad, Abdolhossein; Ismaili, Ahmad

2014-03-30

Using proper growing medium is known to be an effective way to improve crop growth and yield. However, the effects of growing media on geranium essential oil have scarcely ever been examined in detail. In this research, the effects of different growing media (soil, sand, pumice, perlite and perlite + cocopeat) on growth, oil yield and composition of geranium were studied. Growth was significantly improved in soilless-grown plants compared with soil-grown plants. Oil yield of soilless-grown plants (except for pumice) was about threefold higher than that of soil-grown plants. The increase in oil yield was correlated with higher leaf dry weight (r²  = 0.96), as oil content was not affected. The citronellol/geranium ratio of oil was clearly affected by growing media, ranging from 5:1 in soil culture to 3:1 in soilless culture. The latter is acceptable for perfumery. Compared with soil, soilless media could produce higher yields of high-quality geranium oil that fits market requirements. Growth, oil yield and composition of plants grown in sand (a cheap and abundant growing medium) were not significantly different from those of plants grown in perlite and perlite + cocopeat. © 2013 Society of Chemical Industry.

Helping crops stand up to salt

DOE Office of Scientific and Technical Information (OSTI.GOV)

Raeburn, P.

1985-05-01

A new approach to the problem of increasing soil salinity is to raise salt-tolerant plants. The search for such plants involves finding new applications for naturally occurring salt-resistant plants (halophytes), using conventional breeding techniques to identify and strengthen crop varieties known to have better-than-average salt tolerance, and applying recombinant DNA methods to introduce salt resistance into existing plants. One promising plant is salicornia, which produces oil high in polyunsaturates at a greater yield than soybeans. Two varieties of atriplex yield as much animal feed as alfalfa and can be harvested several times a year. Seed companies are supporting the research.

The effect of organic solvent, temperature and mixing time on the production of oil from Moringa oleifera seeds

NASA Astrophysics Data System (ADS)

Ghazali, Q.; Yasin, N. H. M.

2016-06-01

The effect of three different organic solvent, temperature and mixing time on the production of oil from M.oleifera seeds were studied to evaluate the effectiveness in obtaining the high oil yield based on the percentage of oil production. The modified version of Soxhlet extraction method was carried out to extract the oil from M.oleifera seeds by using hexane, heptane and ethanol as the organic solvent. Among the three solvents, it is found that heptane yield higher oil from M.oleifera seeds with maximum oil yield of 36.37% was obtained followed by hexane and ethanol with 33.89% and 18.46%, respectively. By using heptane as a solvent, the temperature (60oC, 70oC, 80oC) and mixing time (6 h, 7 h, and 8 h) were investigated to ensure the high oil yield over the experimental ranges employed and high oil yield was obtained at 600C for 6 h with percentage oil yield of 36.37%. The fatty acid compositions of M.oleifera seeds oil were analyzed using Gas Chromatography/Mass Spectrometry (GC-MS). The main components of fatty acid contained in the oil extracted from M.oleifera seeds was oleic acid, followed by palmitic acid and arachidic acid, and small amount of behenic acid and margaric acid.

Hydrodistillation extraction time effect on essential oil yield, composition, and bioactivity of coriander oil.

PubMed

Zheljazkov, Valtcho D; Astatkie, Tess; Schlegel, Vicki

2014-01-01

Coriander (Coriandrum sativum L.) is a major essential oil crop grown throughout the world. Coriander essential oil is extracted from coriander fruits via hydrodistillation, with the industry using 180-240 min of distillation time (DT), but the optimum DT for maximizing essential oil yield, composition of constituents, and antioxidant activities are not known. This research was conducted to determine the effect of DT on coriander oil yield, composition, and bioactivity. The results show that essential oil yield at the shorter DT was low and generally increased with increasing DT with the maximum yields achieved at DT between 40 and 160 min. The concentrations of the low-boiling point essential oil constituents: α-pinene, camphene, β-pinene, myrcene, para-cymene, limonene, and γ-terpinene were higher at shorter DT (< 2.5 min) and decreased with increasing DT; but the trend reversed for the high-boiling point constituents: geraniol and geranyl-acetate. The concentration of the major essential oil constituent, linalool, was 51% at DT 1.15 min, and increased steadily to 68% with increasing DT. In conclusion, 40 min DT is sufficient to maximize yield of essential oil; and different DT can be used to obtain essential oil with differential composition. Its antioxidant capacity was affected by the DT, with 20 and 240 min DT showing higher antioxidant activity. Comparisons of coriander essential oil composition must consider the length of the DT.

Variations in Volatile Oil Yield and Composition of "Xin-yi" (Magnolia biondii Pamp. Flower Buds) at Different Growth Stages.

PubMed

Hu, Mingli; Bai, Mei; Ye, Wei; Wang, Yaling; Wu, Hong

2018-06-01

Dried flower buds of Magnolia biondii Pamp. are the main ingredient in "Xin-yi" in China, and the volatile oils of M. biondii flower buds are the principal medicinal component. Gas chromatographymass spectrometry (GC-MS) and microscopic techniques were employed to detect the volatile yields of M. biondii flowers at various growth stages. The volatile oil yields of M. biondii flowers differed significantly at different growth stages and were closely related to flower dry weight, oil cell density and degree of oil accumulation. In February 2016, flower buds had the highest dry weight, the maximum percentage of oil cells at the oil saturation stage and the highest density of oil cells, which coincided with the highest oil yield. In March 2016, flower buds had a lower dry weight, a higher percentage of oil cells at the oil-degrading stage and the lowest oil cell density, resulting in decreased oil yields. The total amounts of the major medicinal components in the M. biondii flower also showed regular changes at different growth stages. In January and February of 2016, M. biondii flowers had a higher dry weight, volatile oil yield and total content of medicinal ingredients, which was the best time for harvesting high-quality medicinal components. Our study reveals that volatile oil content and chemical composition are closely related to the growth stage of M. biondii flower buds. The results provide a scientific morphology and composition index for evaluating the medicinal value and harvesting of high-quality M. biondii medicinal herbs.

Has the use of talc an effect on yield and extra virgin olive oil quality?

PubMed

Caponio, Francesco; Squeo, Giacomo; Difonzo, Graziana; Pasqualone, Antonella; Summo, Carmine; Paradiso, Vito Michele

2016-08-01

The maximization of both extraction yield and extra virgin olive oil quality during olive processing are the main objectives of the olive oil industry. As regards extraction yield, it can be improved by both acting on time/temperature of malaxation and using physical coadjuvants. It is well known that, generally, increasing temperature of malaxation gives an increase in oil extraction yield due to a reduction in oily phase viscosity; however, high malaxation temperature can compromise the nutritional and health values of extra virgin olive oil, leading to undesirable effects such as accelerated oxidative process and loss of volatile compounds responsible for oil flavor and fragrance. The addition of physical coadjuvants in olive oil processing during the malaxation phase, not excluded by EC regulations owing to its exclusively physical action, is well known to promote the breakdown of oil/water emulsions and consequently make oil extraction easier, thus increasing the yield. Among physical coadjuvants, micronized natural talc is used for olive oil processing above all for Spanish and Italian olive cultivars. The quality of extra virgin olive oil depends on numerous variables such as olive cultivar, ripeness degree and quality, machines utilized for processing, oil storage conditions, etc. However, the coadjuvants utilized in olive processing can also influence virgin olive oil characteristics. The literature highlights an increase in oil yield by micronized natural talc addition during olive processing, whereas no clear trend was observed as regards the chemical, nutritional and sensory characteristics of extra virgin olive oil. Although an increase in oil stability was reported, no effect of talc was found on the evolution of virgin olive oil quality indices during storage. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

[Supercritical CO2 extraction and component analysis of Aesculus wilsonii seed oil].

PubMed

Chen, Guang-Yu; Shi, Zhao-Hua; Li, Hai-Chi; Ge, Fa-Huan; Zhan, Hua-Shu

2013-03-01

To research the optimal extraction process of supercritical CO2 extraction and analyze the component of the oil extracted from Aesculus wilsonii seed. Using the yield of Aesculus wilsonii seed oil as the index, optimized supercritical CO2 extraction parameter by orthogonal experiment methodology and analysed the compounds of Aesculus wilsonii seed oil by GC-MS. The optimal parameters of the supercritical CO2 extraction of the oil extracted from Aesculus wilsoniit seed were determined: the extraction pressure was 28 MPa and the temperature was 38 degrees C, the separation I pressure was 12 MPa and the temperature was 40 degrees C, the separation II pressure was 5 MPa and the temperature was 40 degrees C, the extraction time was 110 min. The average extraction rate of Aesculus wilsonii seed oil was 1.264%. 26 kinds of compounds were identified by GC-MS in Aesculus wilsonii seed oil extracted by supercritical CO2. The main components were fatty acids. Comparing with the petroleum ether extraction, the supercritical CO2 extraction has higher extraction rate, shorter extraction time, more clarity oil. The kinds of fatty acids with high amounts in Aesculus wilsonii seed oil is identical in general, the kinds of fatty acids with low amounts in Aesculus wilsonii seed oil have differences.

Catalytic pyrolysis of Alcea pallida stems in a fixed-bed reactor for production of liquid bio-fuels.

PubMed

Aysu, Tevfik

2015-09-01

Pyrolysis of Alcea pallida stems was performed in a fixed-bed tubular reactor with and without catalyst at three different temperatures. The effects of pyrolysis parameters including temperature and catalyst on the product yields were investigated. It was found that higher temperature resulted in lower liquid (bio-oil) and solid (bio-char) yields and higher gas yields. Catalysts had different effects on product yields and composition of bio-oils. Liquid yields were increased in the presence of zinc chloride and alumina but decreased with calcium hydroxide, tincal and ulexite. The highest bio-oil yield (39.35%) by weight including aqueous phase was produced with alumina catalyst at 500 °C. The yields of bio-char, bio-oil and gas produced, as well as the compositions of the resulting bio-oils were determined by elemental analysis, TGA, FT-IR and GC-MS. 160 different compounds were identified by GC-MS in the bio-oils obtained at 500 °C. Copyright © 2015 Elsevier Ltd. All rights reserved.

Distillation Time as Tool for Improved Antimalarial Activity and Differential Oil Composition of Cumin Seed Oil

PubMed Central

Zheljazkov, Valtcho D.; Gawde, Archana; Cantrell, Charles L.; Astatkie, Tess; Schlegel, Vicki

2015-01-01

A steam distillation extraction kinetics experiment was conducted to estimate essential oil yield, composition, antimalarial, and antioxidant capacity of cumin (Cuminum cyminum L.) seed (fruits). Furthermore, regression models were developed to predict essential oil yield and composition for a given duration of the steam distillation time (DT). Ten DT durations were tested in this study: 5, 7.5, 15, 30, 60, 120, 240, 360, 480, and 600 min. Oil yields increased with an increase in the DT. Maximum oil yield (content, 2.3 g/100 seed), was achieved at 480 min; longer DT did not increase oil yields. The concentrations of the major oil constituents α-pinene (0.14–0.5% concentration range), β-pinene (3.7–10.3% range), γ-cymene (5–7.3% range), γ-terpinene (1.8–7.2% range), cumin aldehyde (50–66% range), α-terpinen-7-al (3.8–16% range), and β-terpinen-7-al (12–20% range) varied as a function of the DT. The concentrations of α-pinene, β-pinene, γ-cymene, γ-terpinene in the oil increased with the increase of the duration of the DT; α-pinene was highest in the oil obtained at 600 min DT, β-pinene and γ-terpinene reached maximum concentrations in the oil at 360 min DT; γ-cymene reached a maximum in the oil at 60 min DT, cumin aldehyde was high in the oils obtained at 5–60 min DT, and low in the oils obtained at 240–600 min DT, α-terpinen-7-al reached maximum in the oils obtained at 480 or 600 min DT, whereas β-terpinen-7-al reached a maximum concentration in the oil at 60 min DT. The yield of individual oil constituents (calculated from the oil yields and the concentration of a given compound at a particular DT) increased and reached a maximum at 480 or 600 min DT. The antimalarial activity of the cumin seed oil obtained during the 0–5 and at 5–7.5 min DT timeframes was twice higher than the antimalarial activity of the oils obtained at the other DT. This study opens the possibility for distinct marketing and utilization for these improved oils. The antioxidant capacity of the oil was highest in the oil obtained at 30 min DT and lowest in the oil from 360 min DT. The Michaelis-Menton and the Power nonlinear regression models developed in this study can be utilized to predict essential oil yield and composition of cumin seed at any given duration of DT and may also be useful to compare previous reports on cumin oil yield and composition. DT can be utilized to obtain cumin seed oil with improved antimalarial activity, improved antioxidant capacity, and with various compositions. PMID:26641276

Comparison of oilseed yields: a preliminary review

DOE Office of Scientific and Technical Information (OSTI.GOV)

Duke, J.A.; Bagby, M.O.

It was assumed that for most oilseed crops, 90% of the oil yield might be considered as profit. To compare oil seeds, pertinent portions of the yield and energy paragraphs from a summary published by Dr. Duke for DOE Grant No. 59-2246-1-6-054-0 with Dr. Bagby as ADODR were reproduced. The seed yields ranged from 200 to 14,000 kg/ha, the low one too low to consider and the high one suspiciously high. The yield of 14,000 kg oil per hectare is equivalent to more than 30 barrels of oil per hectare. The energy species included ambrette, tung-oil tree, cashew, wood-oil tree,more » mu-oil tree, peanut, mustard greens; rape, colza; black mustard, turnip, safflower, colocynth, coconut, crambe, African oil palm, soybean, cotton, sunflower, Eastern black walnut, Engligh walnut, meadow foam, flax, macadamia nuts, opium poppy, perilla, almond, castorbean, Chinese tallow tree, sesame, jojoba, yellow mustard, stokes' aster, and Zanzibar oilvine. 1 table. (DP)« less

Chemical and toxicological characterization of residential oil burner emissions: I. Yields and chemical characterization of extractables from combustion of No. 2 fuel oil at different Bacharach Smoke Numbers and firing cycles.

PubMed Central

Leary, J A; Biemann, K; Lafleur, A L; Kruzel, E L; Prado, G P; Longwell, J P; Peters, W A

1987-01-01

Particulates and complex organic mixtures were sampled from the exhaust of a flame retention head residential oil burner combusting No. 2 fuel oil at three firing conditions: continuous at Bacharach Smoke No. 1, and cyclic (5 min on, 10 min off) at Smoke Nos. 1 and 5. The complex mixtures were recovered by successive Soxhlet extraction of filtered particulates and XAD-2 sorbent resin with methylene chloride (DCM) and then methanol (MeOH). Bacterial mutagenicity [see Paper II (8)] was found in the DCM extractables. Samples of DCM extracts from the two cyclic firing conditions and of the raw fuel were separated by gravity column chromatography on alumina. The resulting fractions were further characterized by a range of instrumental methods. Average yields of both unextracted particulates and of DCM extractables, normalized to a basis of per unit weight of fuel fired, were lower for continuous firing than for cyclic firing. For cyclic firing, decreasing the smoke number lowered the particulates emissions but only slightly reduced the average yield of DCM extractables. These and similar observations, here reported for two other oil burners, show that adjusting the burner to a lower smoke number has little effect on, or may actually increase, emissions of organic extractables of potential public health interest. Modifications of the burner firing cycle aimed at approaching continuous operation offer promise for reducing the amount of complex organic emissions. Unburned fuel accounted for roughly half of the DCM extractables from cyclic firing of the flame retention head burner at high and low smoke number. Large (i.e., greater than 3 ring) polycyclic aromatic hydrocarbons (PAH) were not observed in the DCM extractables from cyclic firing. However, nitroaromatics, typified by alkylated nitronaphthalenes, alkyl-nitrobiphenyls, and alkyl-nitrophenanthrenes were found in a minor subfraction containing a significant portion of the total mutagenic activity of the cyclic low smoke samples (8). Oxygen-containing PAH, typified by phenalene-1-one and its alkyl derivatives, are important mutagens from cyclic firing at high smoke conditions. Thus, oil burner effluents differ markedly from those of several other combustors, including the automotive diesel engine, where multiring PAH, typified by fluoranthene and alkylated phenanthrenes, account for a significant portion of the effluent mutagenicity. Implications for combustion and emissions source identification are discussed. PMID:3665865

Photochemical dissolution of Turkish lignites in tetralin at different irradiation power and reaction times

DOE Office of Scientific and Technical Information (OSTI.GOV)

F. Karacan; T. Torul

2007-08-15

The effect of the power of ultraviolet (UV) irradiation on the tetrahydrofuran (THF) solubles yield (the total soluble product) and the soluble product distribution of the dissolution of Turkish lignites (Beypazari and Tuncbilek lignite) in tetralin at ambient temperatures has been investigated. The lignite samples were exposed to UV irradiation for 1, 2, 3, 5, and 10 days in the power of irradiation ranging from 0 to 180 W at 60 W intervals. The yields of THF solubles and oils increased with increasing irradiation power and time. The optimum irradiation power depends on the irradiation time to obtain the highestmore » degradation products. However, the yield of degradation products depends also on the lignite type. The largest fraction obtained from lignites by photochemical energy is oil. While the yields of THF solubles and oils sharply increased with irradiation power at longer reaction times, the yields of asphaltenes (AS) slightly decreased. Increasing oil yields is relatively larger when AS yields tend to decrease. These trends of AS and oil yields are ascribable to conversion of AS to oils at higher power. Small changes were observed in the PAS yields under all conditions. 27 refs., 5 figs., 1 tab.« less

Effects of sulfur, zinc, iron, copper, manganese, and boron applications on sunflower yield and plant nutrient concentration

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hilton, B.R.; Zubriski, J.C.

1985-01-01

Sulfur, zinc, iron, copper, manganese, and boron application did not affect the seed yield or oil percentage of sunflower (Helianthus annuus L.) on both dryland and irrigated soils in North Dakota in 1981. Field averages indicated significant Zn, Mn, and B uptake by sunflower at the 12-leaf stage as a result of fertilization with these elements. Increased Zn uptake was also observed in the uppermost mature leaf at anthesis from zinc fertilization. Although sunflower yield from boron fertilization was not significantly different from the check, a trend was observed in which boron fertilization seemed to decrease sunflower yield. Sunflower yieldsmore » from the boron treatment were the lowest out of seven treatments in three out of four fields. Also, sunflower yield from the boron treatment was significantly lower than both iron and sulfur treatments when all fields were combined.« less

Polychlorinated biphenyls (PCBs) contamination and aryl hydrocarbon receptor (AhR) agonist activity of Omega-3 polyunsaturated fatty acid supplements: implications for daily intake of dioxins and PCBs.

PubMed

Bourdon, J A; Bazinet, T M; Arnason, T T; Kimpe, L E; Blais, J M; White, P A

2010-11-01

Omega-3 polyunsaturated fatty acid (n-3 PUFA) rich oils derived primarily from fish are frequently consumed as supplements. Due to the tendency of persistent organic pollutants (POPs) to accumulate in exposed organisms, n-3 PUFA supplements can contain sufficient POPs to present a risk to consumers. Here we investigated PCB concentrations and aryl hydrocarbon receptor (AhR) agonist activity in 17 n-3 PUFA supplements available in Canada. PCBs ranged from <0.8 to 793 ng g(-1) oil, with salmon- and seal-derived products yielding the highest values. AhR agonist activity from a reporter gene assay ranged from 1.3 to 72.2 pg TEQ g(-1) oil, with salmon and tuna yielding the highest values. When consumed at the recommended doses and as a supplement to the average Canadian diet, seal-derived oil can contribute to exceedance of the tolerable daily intake of 20 ng PCBs kg-BW(-1)day(-1), and salmon-, tuna-, and sea herring-derived oils can contribute to exceedance of the tolerable daily intake limit of 2.3 pg TEQ kg-BW(-1)day(-1). The beneficial properties of fish and n-3 PUFA supplements, and the results of this study suggest that it is prudent to consume supplements derived from small, cold-water fatty fish. Further research will be necessary to draw firm conclusions. Crown Copyright © 2010. Published by Elsevier Ltd. All rights reserved.

An investigation of age and yield of fresh fruit bunches of oil palm based on ALOS PALSAR 2

NASA Astrophysics Data System (ADS)

Darmawan, S.; Takeuchi, W.; Haryati, A.; M, R. Najib A.; Na'aim, M.

2016-06-01

The objective on this study is to investigate age and yield of FFB of oil palms based on ALOS PALSAR 2. Study areas in oil palm plantations areas of Jerantut, Pahang Malaysia. Methodology consists collecting of ALOS PALSAR 2 and tabular data on the study area, processing of ALOS PALSAR 2 including of converting digital numbers to normalize radar cross sections (NRCS), topography correction and filtering, making of regions of interest according to areas of age and yield of FFB of oil palms and making of relationship analysis between backscatter value of HH, HV and age and yield of FFB of oil palm. The results have showed relationship between HH, HV and age of oil palm which R2 of 0.63 for HH and 0.42 for HV that indicated increasing of age of oil palm as increasing of HH and HV value. Also relationship between HH, HV and yield of FFB of oil palm which R2 of 0.26 for HH and 0.15 for HV, that indicated increasing of yield of FFB as decreasing of HH and HV value.

A photometric method for the estimation of the oil yield of oil shale

USGS Publications Warehouse

Cuttitta, Frank

1951-01-01

A method is presented for the distillation and photometric estimation of the oil yield of oil-bearing shales. The oil shale is distilled in a closed test tube and the oil extracted with toluene. The optical density of the toluene extract is used in the estimation of oil content and is converted to percentage of oil by reference to a standard curve. This curve is obtained by relating the oil yields determined by the Fischer assay method to the optical density of the toluene extract of the oil evolved by the new procedure. The new method gives results similar to those obtained by the Fischer assay method in a much shorter time. The applicability of the new method to oil-bearing shale and phosphatic shale has been tested.

Sustainable management in crop monocultures: the impact of retaining forest on oil palm yield.

PubMed

Edwards, Felicity A; Edwards, David P; Sloan, Sean; Hamer, Keith C

2014-01-01

Tropical agriculture is expanding rapidly at the expense of forest, driving a global extinction crisis. How to create agricultural landscapes that minimise the clearance of forest and maximise sustainability is thus a key issue. One possibility is protecting natural forest within or adjacent to crop monocultures to harness important ecosystem services provided by biodiversity spill-over that may facilitate production. Yet this contrasts with the conflicting potential that the retention of forest exports dis-services, such as agricultural pests. We focus on oil palm and obtained yields from 499 plantation parcels spanning a total of ≈23,000 ha of oil palm plantation in Sabah, Malaysian Borneo. We investigate the relationship between the extent and proximity of both contiguous and fragmented dipterocarp forest cover and oil palm yield, controlling for variation in oil palm age and for environmental heterogeneity by incorporating proximity to non-native forestry plantations, other oil palm plantations, and large rivers, elevation and soil type in our models. The extent of forest cover and proximity to dipterocarp forest were not significant predictors of oil palm yield. Similarly, proximity to large rivers and other oil palm plantations, as well as soil type had no significant effect. Instead, lower elevation and closer proximity to forestry plantations had significant positive impacts on oil palm yield. These findings suggest that if dipterocarp forests are exporting ecosystem service benefits or ecosystem dis-services, that the net effect on yield is neutral. There is thus no evidence to support arguments that forest should be retained within or adjacent to oil palm monocultures for the provision of ecosystem services that benefit yield. We urge for more nuanced assessments of the impacts of forest and biodiversity on yields in crop monocultures to better understand their role in sustainable agriculture.

Sustainable Management in Crop Monocultures: The Impact of Retaining Forest on Oil Palm Yield

PubMed Central

Edwards, Felicity A.; Edwards, David P.; Sloan, Sean; Hamer, Keith C.

2014-01-01

Tropical agriculture is expanding rapidly at the expense of forest, driving a global extinction crisis. How to create agricultural landscapes that minimise the clearance of forest and maximise sustainability is thus a key issue. One possibility is protecting natural forest within or adjacent to crop monocultures to harness important ecosystem services provided by biodiversity spill-over that may facilitate production. Yet this contrasts with the conflicting potential that the retention of forest exports dis-services, such as agricultural pests. We focus on oil palm and obtained yields from 499 plantation parcels spanning a total of ≈23,000 ha of oil palm plantation in Sabah, Malaysian Borneo. We investigate the relationship between the extent and proximity of both contiguous and fragmented dipterocarp forest cover and oil palm yield, controlling for variation in oil palm age and for environmental heterogeneity by incorporating proximity to non-native forestry plantations, other oil palm plantations, and large rivers, elevation and soil type in our models. The extent of forest cover and proximity to dipterocarp forest were not significant predictors of oil palm yield. Similarly, proximity to large rivers and other oil palm plantations, as well as soil type had no significant effect. Instead, lower elevation and closer proximity to forestry plantations had significant positive impacts on oil palm yield. These findings suggest that if dipterocarp forests are exporting ecosystem service benefits or ecosystem dis-services, that the net effect on yield is neutral. There is thus no evidence to support arguments that forest should be retained within or adjacent to oil palm monocultures for the provision of ecosystem services that benefit yield. We urge for more nuanced assessments of the impacts of forest and biodiversity on yields in crop monocultures to better understand their role in sustainable agriculture. PMID:24638038

Green bio-oil extraction for oil crops

NASA Astrophysics Data System (ADS)

Zainab, H.; Nurfatirah, N.; Norfaezah, A.; Othman, H.

2016-06-01

The move towards a green bio-oil extraction technique is highlighted in this paper. The commonly practised organic solvent oil extraction technique could be replaced with a modified microwave extraction. Jatropha seeds (Jatropha curcas) were used to extract bio-oil. Clean samples were heated in an oven at 110 ° C for 24 hours to remove moisture content and ground to obtain particle size smaller than 500μm. Extraction was carried out at different extraction times 15 min, 30 min, 45 min, 60 min and 120 min to determine oil yield. The biooil yield obtained from microwave assisted extraction system at 90 minutes was 36% while that from soxhlet extraction for 6 hours was 42%. Bio-oil extracted using the microwave assisted extraction (MAE) system could enhance yield of bio-oil compared to soxhlet extraction. The MAE extraction system is rapid using only water as solvent which is a nonhazardous, environment-friendly technique compared to soxhlet extraction (SE) method using hexane as solvent. Thus, this is a green technique of bio-oil extraction using only water as extractant. Bio-oil extraction from the pyrolysis of empty fruit bunch (EFB), a biomass waste from oil palm crop, was enhanced using a biocatalyst derived from seashell waste. Oil yield for non-catalytic extraction was 43.8% while addition of seashell based biocatalyst was 44.6%. Oil yield for non-catalytic extraction was 43.8% while with addition of seashell-based biocatalyst was 44.6%. The pH of bio-oil increased from 3.5 to 4.3. The viscosity of bio-oil obtained by catalytic means increased from 20.5 to 37.8 cP. A rapid and environment friendly extraction technique is preferable to enhance bio-oil yield. The microwave assisted approach is a green, rapid and environmental friendly extraction technique for the production of bio-oil bearing crops.

Exploiting Domain Knowledge to Forecast Heating Oil Consumption

NASA Astrophysics Data System (ADS)

Corliss, George F.; Sakauchi, Tsuginosuke; Vitullo, Steven R.; Brown, Ronald H.

2011-11-01

The GasDay laboratory at Marquette University provides forecasts of energy consumption. One such service is the Heating Oil Forecaster, a service for a heating oil or propane delivery company. Accurate forecasts can help reduce the number of trucks and drivers while providing efficient inventory management by stretching the time between deliveries. Accurate forecasts help retain valuable customers. If a customer runs out of fuel, the delivery service incurs costs for an emergency delivery and often a service call. Further, the customer probably changes providers. The basic modeling is simple: Fit delivery amounts sk to cumulative Heating Degree Days (HDDk = Σmax(0,60 °F—daily average temperature)), with wind adjustment, for each delivery period: sk≈ŝk = β0+β1HDDk. For the first few deliveries, there is not enough data to provide a reliable estimate K = 1/β1 so we use Bayesian techniques with priors constructed from historical data. A fresh model is trained for each customer with each delivery, producing daily consumption forecasts using actual and forecast weather until the next delivery. In practice, a delivery may not fill the oil tank if the delivery truck runs out of oil or the automatic shut-off activates prematurely. Special outlier detection and recovery based on domain knowledge addresses this and other special cases. The error at each delivery is the difference between that delivery and the aggregate of daily forecasts using actual weather since the preceding delivery. Out-of-sample testing yields MAPE = 21.2% and an average error of 6.0% of tank capacity for Company A. The MAPE and an average error as a percentage of tank capacity for Company B are 31.5 % and 6.6 %, respectively. One heating oil delivery company who uses this forecasting service [1] reported instances of a customer running out of oil reduced from about 250 in 50,000 deliveries per year before contracting for our service to about 10 with our service. They delivered slightly more oil with 20 % fewer trucks and drivers, citing 250,000 annual savings in operational costs.

Conversion of secondary pulp/paper sludge powder to liquid oil products for energy recovery by direct liquefaction in hot-compressed water.

PubMed

Xu, Chunbao; Lancaster, Jody

2008-03-01

The present work demonstrated that secondary pulp/paper sludge powder, with a higher heating value of 18.3MJ/kg on a dry basis, could be effectively converted into liquid oil products by direct liquefaction in hot-compressed water with and without catalyst. Treatments of secondary pulp/paper sludge in water at 250-380 degrees C for 15-120min in the presence of N(2) atmosphere resulted in yields of water-soluble oils at 20-45wt% and yields of heavy oils at 15-25wt%, with higher heating values of 10-15 and >35MJ/kg, respectively. The higher caloric values for the heavy oil products were accounted for by their compositions of long-chain carboxylic acids, heterocyclic nitrogen compounds and phenolic compounds and derivatives as evidenced by the gas chromatograph (GC)/MS measurements. The liquefaction product yields were significantly influenced by the liquefaction temperature, the residence time, the initial biomass concentration, catalysts and the liquefaction atmosphere (inert or reducing). Within the temperature range (250-380 degrees C) tested, the lowest temperature produced the highest yield of total oils (at 60wt%), while the greatest yield of heavy oil (at about 24wt%) was obtained at 350 degrees C. If the temperature was fixed at 280 degrees C, a greater yield of heavy oil (reaching as high as 25wt% for 120min) was obtained as the length of reaction time increased. Similarly, a higher initial biomass concentration produced a greater yield of heavy oil but a reduced yield of water-soluble oil. The presence of 0.1M K(2)CO(3) dramatically enhanced organic conversion, but suppressed the formation of both heavy oil and water-soluble oil. The use of the two alkaline earth metal catalysts, i.e., Ca(OH)(2) and Ba(OH)(2), did not alter organic conversion, but it catalyzed the formation of water-soluble oil and produced higher yields of total oil products. It was also demonstrated that the reducing atmosphere (i.e., H(2)) in the liquefaction process promoted the heavy oil formation while suppressing the water-soluble oil formation. With the presence of 0.1M Ca(OH)(2) and 2MPa H(2), liquefaction of the sludge powder in water at 280 degrees C for 60min produced a higher yield of heavy oil (26wt%), almost two times as high as that in N(2) (13.6wt%), resulting in a greater net energy efficiency. It was thus suggested that direct liquefaction of secondary pulp/paper sludge in hot-compressed water with Ca(OH)(2) catalyst and in the presence of H(2) could be an effective approach to recovering energy from the waste for production of liquid oil products.

Effect of sodium carbonate catalyst weight on production of bio-oil via thermochemical liquefaction of corncobs in ethanol-water solution

NASA Astrophysics Data System (ADS)

Sembodo, Bregas Siswahjono Tatag; Sulistyo, Hary; Sediawan, Wahyudi Budi; Fahrurrozi, Mohammad

2018-02-01

Lignocellulosic biomass has recently received serious attention as an energy source that can replace fossil fuels. Corncob is one of lignocellulosic biomass wastes, which can be further processed into bio-oil through thermochemical liquefaction process. Bio-oil is expected to be further processed into fuel oil. In this research the effect of Na2CO3 catalyst weight on the yield of bio-oil was investigated. The composition of bio-oil produced in this process was analyzed by GC-MS. Bio-oil formation rate were analyzed through mathematical model development. First model aasumed as an isothermal process, while second model was not. It is found that both models were able to provide a good approach to experimental data. The average reaction rate constants was obtained from isothermal model, while the activation energy level and collision factors were obtained from non-isothermal model. The reaction rate will increase by addition of Na2CO3 (0 - 0.5 g) as catalyst to 250 mL system solution, then the activation energy will decrease from 1964.265 joules/mole to 1029.994 joules/mole. The GC-MS analysis results showed that the bio-oil were contained of ester compounds, phenolic compounds, cyclic compunds, heterocyclic compounds, and poly-alcohols compounds.

The effect of clay catalyst on the chemical composition of bio-oil obtained by co-pyrolysis of cellulose and polyethylene.

PubMed

Solak, Agnieszka; Rutkowski, Piotr

2014-02-01

Cellulose/polyethylene (CPE) mixture 3:1, w/w with and without three clay catalysts (K10 - montmorillonite K10, KSF - montmorillonite KSF, B - Bentonite) addition were subjected to pyrolysis at temperatures 400, 450 and 500°C with heating rate of 100°C/s to produce bio-oil with high yield. The pyrolytic oil yield was in the range of 41.3-79.5 wt% depending on the temperature, the type and the amount of catalyst. The non-catalytic fast pyrolysis at 500°C gives the highest yield of bio-oil (79.5 wt%). The higher temperature of catalytic pyrolysis of cellulose/polyethylene mixture the higher yield of bio-oil is. Contrarily, increasing amount of montmorillonite results in significant, almost linear decrease in bio-oil yield followed by a significant increase of gas yield. The addition of clay catalysts to CPE mixture has a various influence on the distribution of bio-oil components. The addition of montmorillonite K10 to cellulose/polyethylene mixture promotes the deepest conversion of polyethylene and cellulose. Additionally, more saturated than unsaturated hydrocarbons are present in resultant bio-oils. The proportion of liquid hydrocarbons is the highest when a montmorillonite K10 is acting as a catalyst. Copyright © 2013 Elsevier Ltd. All rights reserved.

Characterization of Essential Oil Composition in Different Basil Species and Pot Cultures by a GC-MS Method.

PubMed

Muráriková, Andrea; Ťažký, Anton; Neugebauerová, Jarmila; Planková, Alexandra; Jampílek, Josef; Mučaji, Pavel; Mikuš, Peter

2017-07-20

Basil ( Ocimum L.) species are used as medicinal plants due to their essential oils exhibiting specific biological activity. The present work demonstrated that both the variety and season/conditions of cultivation had a significant effect on (i) the produced amount (extraction yield), (ii) qualitative, as well as (iii) quantitative profile of basil essential oil. Among studied basil varieties, a new variety, 'Mánes', was characterized for the first time. Based on our quantitative evaluation of GC-MS profiles, the following chemotypes and average concentrations of a main component were detected in the studied basil varieties: 'Ohře', 'Lettuce Leaf', 'Purple Opaal', 'Dark Green' (linalool, 5.99, 2.49, 2.34, 2.01 mg/mL, respectively), and 'Mammolo Genovese', 'Mánes', 'Red Rubin' (eucalyptol, 1.34, 0.96, 0.76 mg/mL, respectively). At the same time, when considering other compounds identified in GC-MS profiles, all the studied varieties, except from 'Lettuce Leaf', were methyl eugenol-rich with a strong dependence of the eugenol:methyl eugenol ratio on the seasonal changes (mainly solar irradiation, but also temperature and relative humidity). More complex and/or variable (depending on the season and cultivation) chemotypes were observed with 'Lettuce Leaf' (plus estragole, 2.27 mg/mL), 'Dark Green' (plus eucalyptol, 1.36 mg/mL), 'Mammolo Genovese' (plus eugenol, 1.19 mg/mL), 'Red Rubin' (plus linalool and eugenol, 0.46 and 0.56 mg/mL, respectively), and 'Mánes' (plus linalool and eugenol, 0.58 and 0.40 mg/mL, respectively). When considering superior extraction yield (ca. 17 mL·kg -1 , i.e., two to five times higher than other examined varieties) and consistent amounts (yields) of essential oil when comparing inter-seasonal or inter-year data (RSD and inter-year difference in mean yield values ˂2.5%), this new basil variety is very promising for use in the pharmaceutical, food, and cosmetic industries.

Simulation of Oil Palm Shell Pyrolysis to Produce Bio-Oil with Self-Pyrolysis Reactor

NASA Astrophysics Data System (ADS)

Fika, R.; Nelwan, L. O.; Yulianto, M.

2018-05-01

A new self-pyrolysis reactor was designed to reduce the utilization of electric heater due to the energy saving for the production of bio-oil from oil palm shell. The yield of the bio- oil was then evaluated with the developed mathematical model by Sharma [1] with the characteristic of oil palm shell [2]. During the simulation, the temperature on the combustion chamber on the release of the bio-oil was utilized to determine the volatile composition from the combustion of the oil palm shell as fuel. The mass flow was assumed constant for three experiments. The model resulted in a significant difference between the simulated bio-oil and experiments. The bio-oil yields from the simulation were 22.01, 16.36, and 21.89 % (d.b.) meanwhile the experimental yields were 10.23, 9.82, and 8.41% (d.b.). The char yield varied from 30.7 % (d.b.) from the simulation to 40.9 % (d.b.) from the experiment. This phenomenon was due to the development of process temperature over time which was not considered as one of the influential factors in producing volatile matters on the simulation model. Meanwhile the real experiments highly relied on the process conditions (reactor type, temperature over time, gas flow). There was also possibilities of the occurrence of the gasification inside the reactor which caused the liquid yield was not as high as simulated. Further simulation model research on producing the bio-oil yield will be needed to predict the optimum condition and temperature development on the newly self-pyrolysis reactor.

[Bio-oil production from biomass pyrolysis in molten salt].

PubMed

Ji, Dengxiang; Cai, Tengyue; Ai, Ning; Yu, Fengwen; Jiang, Hongtao; Ji, Jianbing

2011-03-01

In order to investigate the effects of pyrolysis conditions on bio-oil production from biomass in molten salt, experiments of biomass pyrolysis were carried out in a self-designed reactor in which the molten salt ZnCl2-KCl (with mole ratio 7/6) was selected as heat carrier, catalyst and dispersion agent. The effects of metal salt added into ZnCl2-KCl and biomass material on biomass pyrolysis were discussed, and the main compositions of bio-oil were determined by GC-MS. Metal salt added into molten salt could affect pyrolysis production yields remarkably. Lanthanon salt could enhance bio-oil yield and decrease water content in bio-oil, when mole fraction of 5.0% LaCl3 was added, bio-oil yield could reach up to 32.0%, and water content of bio-oil could reduce to 61.5%. The bio-oil and char yields were higher when rice straw was pyrolysed, while gas yield was higher when rice husk was used. Metal salts showed great selectivity on compositions of bio-oil. LiCl and FeCl2 promoted biomass to pyrolyse into smaller molecular weight compounds. CrCl3, CaCl2 and LaCl3 could restrain second pyrolysis of bio-oil. The research provided a scientific reference for production of bio-oil from biomass pyrolysis in molten salt.

Differential abundance analysis of mesocarp protein from high- and low-yielding oil palms associates non-oil biosynthetic enzymes to lipid biosynthesis.

PubMed

Ooi, Tony Eng Keong; Yeap, Wan Chin; Daim, Leona Daniela Jeffery; Ng, Boon Zean; Lee, Fong Chin; Othman, Ainul Masni; Appleton, David Ross; Chew, Fook Tim; Kulaveerasingam, Harikrishna

2015-01-01

The oil palm Elaeis guineensis Jacq. which produces the highest yield per unit land area of the oil crops is the most important commercial oil crop in South East Asia. The fleshy mesocarp of oil palm fruit, where oil is mostly derived from, contains up to 90 % dry weight of oil (one of the most concentrated in plant tissues). Hence, there is attention given to gain insights into the processes of oil deposition in this oil rich tissue. For that purpose, two-dimensional differential gel electrophoresis (DIGE) coupled with western assays, were used here to analyze differential protein levels in genetically-related high-and low-yielding oil palm mesocarps. From the DIGE comparative analysis in combination with western analysis, 41 unique differentially accumulated proteins were discovered. Functional categorization of these proteins placed them in the metabolisms of lipid, carbohydrate, amino acids, energy, structural proteins, as well as in other functions. In particular, higher abundance of fructose-1,6-biphosphate aldolase combined with reduced level of triosephosphate isomerase and glyceraldehyde-3-phosphate dehydrogenase may be indicative of important flux balance changes in glycolysis, while amino acid metabolism also appeared to be closely linked with oil yield. Forty-one proteins in several important biological pathways were identified as exhibiting differential in abundance at critical oil production stages. These confirm that oil yield is a complex trait involving the regulation of genes in multiple biological pathways. The results also provide insights into key control points of lipid biosynthesis in oil palm and can assist in the development of genetic markers for use in oil palm breeding programmes.

Genetic control of soybean seed oil: II. QTL and genes that increase oil concentration without decreasing protein or with increased seed yield.

PubMed

Eskandari, Mehrzad; Cober, Elroy R; Rajcan, Istvan

2013-06-01

Soybean [Glycine max (L.) Merrill] seed oil is the primary global source of edible oil and a major renewable and sustainable feedstock for biodiesel production. Therefore, increasing the relative oil concentration in soybean is desirable; however, that goal is complex due to the quantitative nature of the oil concentration trait and possible effects on major agronomic traits such as seed yield or protein concentration. The objectives of the present study were to study the relationship between seed oil concentration and important agronomic and seed quality traits, including seed yield, 100-seed weight, protein concentration, plant height, and days to maturity, and to identify oil quantitative trait loci (QTL) that are co-localized with the traits evaluated. A population of 203 F4:6 recombinant inbred lines, derived from a cross between moderately high oil soybean genotypes OAC Wallace and OAC Glencoe, was developed and grown across multiple environments in Ontario, Canada, in 2009 and 2010. Among the 11 QTL associated with seed oil concentration in the population, which were detected using either single-factor ANOVA or multiple QTL mapping methods, the number of QTL that were co-localized with other important traits QTL were six for protein concentration, four for seed yield, two for 100-seed weight, one for days to maturity, and one for plant height. The oil-beneficial allele of the QTL tagged by marker Sat_020 was positively associated with seed protein concentration. The oil favorable alleles of markers Satt001 and GmDGAT2B were positively correlated with seed yield. In addition, significant two-way epistatic interactions, where one of the interacting markers was solely associated with seed oil concentration, were identified for the selected traits in this study. The number of significant epistatic interactions was seven for yield, four for days to maturity, two for 100-seed weight, one for protein concentration, and one for plant height. The identified molecular markers associated with oil-related QTL in this study, which also have positive effects on other important traits such as seed yield and protein concentration, could be used in the soybean marker breeding programs aimed at developing either higher seed yield and oil concentration or higher seed protein and oil concentration per hectare. Alternatively, selecting complementary parents with greater breeding values due to positive epistatic interactions could lead to the development of higher oil soybean cultivars.

Advances of two-stage riser catalytic cracking of heavy oil for maximizing propylene yield (TMP) process.

PubMed

Chaohe, Yang; Xiaobo, Chen; Jinhong, Zhang; Chunyi, Li; Honghong, Shan

Two-stage riser catalytic cracking of heavy oil for maximizing propylene yield (TMP) process proposed by State Key Laboratory of Heavy oil Processing, China University of Petroleum, can remarkably enhance the propylene yield and minimize the dry gas and coke yields, and obtain high-quality light oils (gasoline and diesel). It has been commercialized since 2006. Up to now, three TMP commercial units have been put into production and other four commercial units are under design and construction. The commercial data showed that taking paraffinic based Daqing (China) atmospheric residue as the feedstock, the propylene yield reached 20.31 wt%, the liquid products yield (the total yield of liquefied petroleum gas, gasoline, and diesel) was 82.66 wt%, and the total yield of dry gas and coke was 14.28 wt%. Moreover, the research octane number of gasoline could be up to 96.

[Prediction of the side-cut product yield of atmospheric/vacuum distillation unit by NIR crude oil rapid assay].

PubMed

Wang, Yan-Bin; Hu, Yu-Zhong; Li, Wen-Le; Zhang, Wei-Song; Zhou, Feng; Luo, Zhi

2014-10-01

In the present paper, based on the fast evaluation technique of near infrared, a method to predict the yield of atmos- pheric and vacuum line was developed, combined with H/CAMS software. Firstly, the near-infrared (NIR) spectroscopy method for rapidly determining the true boiling point of crude oil was developed. With commercially available crude oil spectroscopy da- tabase and experiments test from Guangxi Petrochemical Company, calibration model was established and a topological method was used as the calibration. The model can be employed to predict the true boiling point of crude oil. Secondly, the true boiling point based on NIR rapid assay was converted to the side-cut product yield of atmospheric/vacuum distillation unit by H/CAMS software. The predicted yield and the actual yield of distillation product for naphtha, diesel, wax and residual oil were compared in a 7-month period. The result showed that the NIR rapid crude assay can predict the side-cut product yield accurately. The near infrared analytic method for predicting yield has the advantages of fast analysis, reliable results, and being easy to online operate, and it can provide elementary data for refinery planning optimization and crude oil blending.

Integrated utilization of red radish seeds for the efficient production of seed oil and sulforaphene.

PubMed

Zhang, Jie; Zhou, Xing; Fu, Min

2016-02-01

Supercritical CO2 was used to obtain seed oil from red radish seeds. The influence of pressure, temperature, CO2 flow rate and time on extraction yield of oil were investigated in detail. The maximum extraction yield of oil was 92.07 ± 0.76% at the optimal extraction conditions. The physicochemical properties and fatty acid composition of oil indicated that the seed oil can be used as a dietary oil. Meanwhile, the high purity sulforaphene (96.84 ± 0.17%) was separated by solvent extraction coupled with preparative high performance liquid chromatography from red radish seed meal. The initial pH, R, extraction temperature and extraction time for each cycle had a considerable influence both on the extraction yield and purity of sulforaphene of crude product. The extraction of oil was directly responsible for an increase of 18.32% in the yield of sulforaphene. Copyright © 2015 Elsevier Ltd. All rights reserved.

Influence of biochemical composition during hydrothermal liquefaction of algae on product yields and fuel properties.

PubMed

Shakya, Rajdeep; Adhikari, Sushil; Mahadevan, Ravishankar; Shanmugam, Saravanan R; Nam, Hyungseok; Hassan, El Barbary; Dempster, Thomas A

2017-11-01

Hydrothermal liquefaction (HTL) of nine algae species were performed at two reaction temperatures (280 and 320°C) to compare the effect of their biomass composition on product yields and properties. Results obtained after HTL indicate large variations in terms of bio-oil yields and its properties. The maximum bio-oil yield (66wt%) was obtained at 320°C with a high lipid containing algae Nannochloropsis. The higher heating value of bio-oils ranged from 31 to 36MJ/kg and around 50% of the bio-oils was in the vacuum gas oil range while high lipid containing algae Nannochloropsis contained a significant portion (33-42%) in the diesel range. A predictive relationship between bio-oil yields and biochemical compositions was developed and showed a broad agreement between predictive and experimental yields. The aqueous phases obtained had high amount of TOC (12-43g/L), COD (35-160g/L), TN (1-18g/L), ammonium (0.34-12g/L) and phosphate (0.7-12g/L). Copyright © 2017 Elsevier Ltd. All rights reserved.

Evaluation of core data, physical properties, and oil yield USBM/AEC Colorado Core Hole no. 3 (Bronco BR-1)

USGS Publications Warehouse

Ege, John R.; Carroll, R.D.; Way, R.J.; Magner, J.E.

1969-01-01

USBM/AEC Colorado Core Hole No. 3 (Bronco BR-1) is located in the SW1/4SW1/4SW1/4 sec. 14, T. 1 N., R. 98 W., Rio Blanco County, Colorado. The collar is at a ground elevation of 6,356 feet. The hole was core drilled between depths of 964 and 3,325 feet with a total depth of 3,797 feet. The hole was drilled to investigate geologic, geophysical and hydrological conditions at a possible in situ oil-shale retorting experiment site. The drill hole passed through 1,157 feet of alluvium and the Evacuation Creek Member of the Green River Formation, 1,603 feet of the Parachute Creek Member and penetrated into the Garden Gulch Member of the Green River Formation. In-bole density log/oil yield ratio interpretation indicates that two oil-shale zones exist which yield more than 20 gallons of shale oil per ton of rock; an upper zone lying between 1,271 and 1,750 feet in depth and a lower zone lying between 1,900 and 2,964 feet. Halite (sodium chloride salt) is found between 2,140 and 2,185 feet and nahcolite (sodium bicarbonate salt) between 2,195 and 2,700 feet. Nahcolite was present at one time above 2,195 feet but has been subsequently dissolved out by ground water. The core can be divided into six structural units based upon degree of fracturing. A highly fractured interval is found between 1,646 and 1,899 feet, which coincides with the dissolution or leached nahcolite zone. Physical property tests made on core samples between 1,356 and 3,253 feet give average values of 11,988 psi for uniaxial compressive strength, 1.38 X 10[superscript]6[superscript] psi for static Young's modulus and 11,809 fps for compressional velocity.

Effect of the corn breaking method on oil distribution between stillage phases of dry-grind corn ethanol production.

PubMed

Wang, H; Wang, T; Johnson, L A; Pometto, A L

2008-11-12

The majority of fuel ethanol in the United States is produced by using the dry-grind corn ethanol process. The corn oil that is contained in the coproduct, distillers' dried grains with solubles (DDGS), can be recovered for use as a biodiesel feedstock. Oil removal will also improve the feed quality of DDGS. The most economical way to remove oil is considered to be at the centrifugation step for separating thin stillage (liquid) from coarse solids after distilling the ethanol. The more oil there is in the liquid, the more it can be recovered by centrifugation. Therefore, we studied the effects of corn preparation and grinding methods on oil distribution between liquid and solid phases. Grinding the corn to three different particle sizes, flaking, flaking and grinding, and flaking and extruding were used to break up the corn kernel before fermentation, and their effects on oil distribution between the liquid and solid phases were examined by simulating an industrial decanter centrifuge. Total oil contents were measured in the liquid and solids after centrifugation. Dry matter yield and oil partitioning in the thin stillage were highly positively correlated. Flaking slightly reduced bound fat. The flaked and then extruded corn meal released the highest amount of free oil, about 25% compared to 7% for the average of the other treatments. The freed oil from flaking, however, became nonextractable after the flaked corn was ground. Fine grinding alone had little effect on oil partitioning.

The influence of purge times on the yields of essential oil components extracted from plants by pressurized liquid extraction.

PubMed

Wianowska, Dorota

2014-01-01

The influence of different purge times on the yield of the main essential oil constituents of rosemary (Rosmarinus officinalis L.), thyme (Thymus vulgaris L.), and chamomile (Chamomilla recutita L.) was investigated. The pressurized liquid extraction process was performed by applying different extraction temperatures and solvents. The results presented in the paper show that the estimated yield of essential oil components extracted from the plants in the pressurized liquid extraction process is purge time-dependent. The differences in the estimated yields are mainly connected with the evaporation of individual essential oil components and the applied solvent during the purge; the more volatile an essential oil constituent is, the greater is its loss during purge time, and the faster the evaporation of the solvent during the purge process is, the higher the concentration of less volatile essential oil components in the pressurized liquid extraction receptacle. The effect of purge time on the estimated yield of individual essential oil constituents is additionally differentiated by the extraction temperature and the extraction ability of the applied solvent.

Elf cites 5 advantages of horizontal drilling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1984-06-01

ELF Aquitaine used horizontal drilling during a pilot test program to bring commercial production from its Rospo Mare oil discovery in the Adriatic, which would have been a costly disappointment if drilled by a conventional vertical well bore. Rospo Mare is a large reservoir containing a top column of highly viscous crude underlain by a water column. The company felt that a well bore that penetrated the reservoir vertically would bring early flooding of the oil column and yield only water. By penetrating the reservoir with a horizontal well drilled high in the oil column, the well successfully produced onmore » numerous tests from Oct. 1982 until the end of the test program in 1983. Production was termed excellent, with productivity during tests reportedly reaching ca 15 times the rate produced from nearby vertical wells. However, ELF said the results usually average ca 5 times the usual rate of vertical wells.« less

Highly oil-producing microalgae selected through directed-evolution on a microfludic chip

NASA Astrophysics Data System (ADS)

Mestler, Troy; Estevez-Torres, Andre; Lambert, Guillaume; Austin, Robert H.

2009-03-01

Some species of photosynthetic microalgae produce signi?cant amounts of oil which can be easily converted to diesel fuel. However, as it stands today, biodiesel is signi?cantly more expensive than fossil fuels. We wish to improve the oil yield and production rate of a single species of microalgae through directed evolution. We propose to utilize our microfabication technology to create microhabitats to control the nutrient environment of the species, monitor oil production through Raman Spectroscopy, and punish colonies of algae which have low oil yield. We believe this process will produce a mutant species with a high oil yield.

Acid--chlorite pretreatment and liquefaction of cornstalk in hot-compressed water for bio-oil production.

PubMed

Liu, Hua-Min; Feng, Bing; Sun, Run-Cang

2011-10-12

In this study, cornstalk was pretreated by an acid-chlorite delignification procedure to enhance the conversion of cornstalk to bio-oil in hot-compressed water liquefaction. The effects of the pretreatment conditions on the compositional and structural changes of the cornstalk and bio-oil yield were investigated. It was found that acid-chlorite pretreatment changed the main components and physical structures of cornstalk and effectively enhanced the bio-oil yield. Shorter residence time favored production of the total bio-oil products, whereas longer time led to cracking of the products. A high water loading was found to be favorable for high yields of total bio-oil and water-soluble oil. GC-MS analysis showed that the water-soluble oil and heavy oil were the complicated products of C(5-10) and C(8-11) organic compounds.

Conversion of cornstalk to bio-oil in hot-compressed water: effects of ultrasonic pretreatment on the yield and chemical composition of bio-oil, carbon balance, and energy recovery.

PubMed

Shi, Wen; Gao, Yahui; Yang, Guohui; Zhao, Yaping

2013-08-07

An ultrasonic pretreatment method was developed to enhance the yield of bio-oil obtained from the liquefaction of cornstalks in hot-compressed water at different reaction temperatures (260-340 °C) and residence times (0-40 min). Influences of ultrasonic pretreatment on the physicochemical properties of cornstalks and bio-oil yields were investigated. The results show that ultrasonic pretreatment obviously increases surface areas of cornstalks, decreases crystallinities, and erodes the structures of lignin, leading to more exposure of cellulose and hemicellulose. The yield of bio-oil was increased remarkably by 10.1% for 40 min sonicated cornstalks under the optimum liquefied conditions (300 °C for 0 min of residence time). Carbon balance indicates that ultrasonic pretreatment increases the carbon conversion of cornstalks to heavy oil and water-soluble oil. Energy balance indicates that the sonicated cornstalks have positive energy efficiencies. GC-MS analyses demonstrate ultrasonic pretreatment increases the contents of the phenols in heavy oil and water-soluble oil.

Study of optimal extraction conditions for achieving high yield and antioxidant activity of tomato seed oil.

PubMed

Shao, Dongyan; Atungulu, Griffiths G; Pan, Zhongli; Yue, Tianli; Zhang, Ang; Li, Xuan

2012-08-01

Value of tomato seed has not been fully recognized. The objectives of this research were to establish suitable processing conditions for extracting oil from tomato seed by using solvent, determine the impact of processing conditions on yield and antioxidant activity of extracted oil, and elucidate kinetics of the oil extraction process. Four processing parameters, including time, temperature, solvent-to-solid ratio and particle size were studied. A second order model was established to describe the oil extraction process. Based on the results, increasing temperature, solvent-to-solid ratio, and extraction time increased oil yield. In contrast, larger particle size reduced the oil yield. The recommended oil extraction conditions were 8 min of extraction time at temperature of 25 °C, solvent-to-solids ratio of 5/1 (v/w) and particle size of 0.38 mm, which gave oil yield of 20.32% with recovery rate of 78.56%. The DPPH scavenging activity of extracted oil was not significantly affected by the extraction parameters. The inhibitory concentration (IC(50) ) of tomato seed oil was 8.67 mg/mL which was notably low compared to most vegetable oils. A 2nd order model successfully described the kinetics of tomato oil extraction process and parameters of extraction kinetics including initial extraction rate (h), equilibrium concentration of oil (C(s) ), and the extraction rate constant (k) could be precisely predicted with R(2) of at least 0.957. The study revealed that tomato seed which is typically treated as a low value byproduct of tomato processing has great potential in producing oil with high antioxidant capability. The impact of processing conditions including time, temperature, solvent-to-solid ratio and particle size on yield, and antioxidant activity of extracted tomato seed oil are reported. Optimal conditions and models which describe the extraction process are recommended. The information is vital for determining the extraction processing conditions for industrial production of high quality tomato seed oil. Journal of Food Science © 2012 Institute of Food Technologists® No claim to original US government works.

Distillation time effect on lavender essential oil yield and composition.

PubMed

Zheljazkov, Valtcho D; Cantrell, Charles L; Astatkie, Tess; Jeliazkova, Ekaterina

2013-01-01

Lavender (Lavandula angustifolia Mill.) is one of the most widely grown essential oil crops in the world. Commercial extraction of lavender oil is done using steam distillation. The objective of this study was to evaluate the effect of the length of the distillation time (DT) on lavender essential oil yield and composition when extracted from dried flowers. Therefore, the following distillation times (DT) were tested in this experiment: 1.5 min, 3 min, 3.75 min, 7.5 min, 15 min, 30 min, 60 min, 90 min, 120 min, 150 min, 180 min, and 240 min. The essential oil yield (range 0.5-6.8%) reached a maximum at 60 min DT. The concentrations of cineole (range 6.4-35%) and fenchol (range 1.7-2.9%) were highest at the 1.5 min DT and decreased with increasing length of the DT. The concentration of camphor (range 6.6-9.2%) reached a maximum at 7.5-15 min DT, while the concentration of linalool acetate (range 15-38%) reached a maximum at 30 min DT. Results suggest that lavender essential oil yield may not increase after 60 min DT. The change in essential oil yield, and the concentrations of cineole, fenchol and linalool acetate as DT changes were modeled very well by the asymptotic nonlinear regression model. DT may be used to modify the chemical profile of lavender oil and to obtain oils with differential chemical profiles from the same lavender flowers. DT must be taken into consideration when citing or comparing reports on lavender essential oil yield and composition.

Field-to-Fuel Performance Testing of Lignocellulosic Feedstocks for Fast Pyrolysis and Upgrading: Techno-economic Analysis and Greenhouse Gas Life Cycle Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meyer, Pimphan A.; Snowden-Swan, Lesley J.; Rappé, Kenneth G.

This work shows preliminary results from techno-economic analysis and life cycle greenhouse gas analysis of the conversion of seven (7) biomass feedstocks to produce liquid transportation fuels via fast pyrolysis and upgrading via hydrodeoxygenation. The biomass consists of five (5) pure feeds (pine, tulip poplar, hybrid poplar, switchgrass, corn stover) and two blends. Blend 1 consists of equal weights of pine, tulip poplar and switchgrass, and blend 2 is 67% pine and 33% hybrid poplar. Upgraded oil product yield is one of the most significant parameters affecting the process economics, and is a function of both fast pyrolysis oil yieldmore » and hydrotreating oil yield. Pure pine produced the highest overall yield, while switchgrass produced the lowest. Interestingly, herbaceous materials blended with woody biomass performed nearly as well as pure woody feedstock, suggesting a non-trivial relationship between feedstock attributes and production yield. Production costs are also highly dependent upon hydrotreating catalyst-related costs. The catalysts contribute an average of ~15% to the total fuel cost, which can be reduced through research and development focused on achieving performance at increased space velocity (e.g., reduced catalyst loading) and prolonging catalyst lifetime. Green-house-gas reduction does not necessarily align with favorable economics. From the greenhouse gas analysis, processing tulip poplar achieves the largest GHG emission reduction relative to petroleum (~70%) because of its lower hydrogen consumption in the upgrading stage that results in a lower natural gas requirement for hydrogen production. Conversely, processing blend 1 results in the smallest GHG emission reduction from petroleum (~58%) because of high natural gas demand for hydrogen production.« less

Diurnal effects on spearmint oil yields and composition

USDA-ARS?s Scientific Manuscript database

‘Native’ spearmint (Mentha spicata L.) is one of the two spearmint species grown commercially in the United States and other countries for essential oil production. The two major constituents of spearmint oil are carvone and limonene. It is not known if the essential oil yield (content) and composit...

Distillation time as tool for improved antimalarial activity and differential oil composition of cumin seed oil

USDA-ARS?s Scientific Manuscript database

A steam distillation extraction kinetics experiment was conducted to estimate essential oil yield, composition, antimalarial, and antioxidant capacity of cumin (Cuminum cyminum L.) seed (fruits). Furthermore, regression models were developed to predict essential oil yield and composition for a given...

Chemometric investigation of light-shade effects on essential oil yield and morphology of Moroccan Myrtus communis L.

PubMed

Fadil, Mouhcine; Farah, Abdellah; Ihssane, Bouchaib; Haloui, Taoufik; Lebrazi, Sara; Zghari, Badreddine; Rachiq, Saâd

2016-01-01

To investigate the effect of environmental factors such as light and shade on essential oil yield and morphological traits of Moroccan Myrtus communis, a chemometric study was conducted on 20 individuals growing under two contrasting light environments. The study of individual's parameters by principal component analysis has shown that essential oil yield, altitude, and leaves thickness were positively correlated between them and negatively correlated with plants height, leaves length and leaves width. Principal component analysis and hierarchical cluster analysis have also shown that the individuals of each sampling site were grouped separately. The one-way ANOVA test has confirmed the effect of light and shade on essential oil yield and morphological parameters by showing a statistically significant difference between them from the shaded side to the sunny one. Finally, the multiple linear model containing main, interaction and quadratic terms was chosen for the modeling of essential oil yield in terms of morphological parameters. Sun plants have a small height, small leaves length and width, but they are thicker and richer in essential oil than shade plants which have shown almost the opposite. The highlighted multiple linear model can be used to predict essential oil yield in the studied area.

Developmental changes in growth, yield and volatile oil of some chinese garlic lines in comparison with the local cultivar "Balady".

PubMed

Abouziena, H F; El-Saeid, Hamed M

2013-10-15

Balady cultivar and six Chinese lines were planted to study their developmental growth, yield and essential oil variations. Bulb of Balady cultivar had more two folds of cloves number per bulb than the Chinese lines. On the contrary Balady cv had the lowest clove weight compared to all Chinese lines. Chinese lines significantly surppassed the Balady cultivar in the bulb yield ha(-1). The bulb yield ha(-1) could be arrangement in descending order as follow Line B > Line F > Line D > Line C > Line A > Line E > Balady cv. Line B significantly surpassed the other tested lines in oil yield and had 7 folds oil yield plant(-1) than the local cultivar. The main compound in the bulb was found to be methylallay disulfide in both Chinese lines and Balady cultivar. Some components which found in the garlic bulbs at the age 150 days disappeared at the maturity time. Chinese Line B recorded the highest bulb yield and volatile oil content comparing with other lines.

The effect of clay catalyst on the chemical composition of bio-oil obtained by co-pyrolysis of cellulose and polyethylene

DOE Office of Scientific and Technical Information (OSTI.GOV)

Solak, Agnieszka; Rutkowski, Piotr, E-mail: piotr.rutkowski@pwr.wroc.pl

2014-02-15

Highlights: • Non-catalytic and catalytic fast pyrolysis of cellulose/polyethylene blend was carried out in a laboratory scale reactor. • Optimization of process temperature was done. • Optimization of clay catalyst type and amount for co-pyrolysis of cellulose and polyethylene was done. • The product yields and the chemical composition of bio-oil was investigated. - Abstract: Cellulose/polyethylene (CPE) mixture 3:1, w/w with and without three clay catalysts (K10 – montmorillonite K10, KSF – montmorillonite KSF, B – Bentonite) addition were subjected to pyrolysis at temperatures 400, 450 and 500 °C with heating rate of 100 °C/s to produce bio-oil with highmore » yield. The pyrolytic oil yield was in the range of 41.3–79.5 wt% depending on the temperature, the type and the amount of catalyst. The non-catalytic fast pyrolysis at 500 °C gives the highest yield of bio-oil (79.5 wt%). The higher temperature of catalytic pyrolysis of cellulose/polyethylene mixture the higher yield of bio-oil is. Contrarily, increasing amount of montmorillonite results in significant, almost linear decrease in bio-oil yield followed by a significant increase of gas yield. The addition of clay catalysts to CPE mixture has a various influence on the distribution of bio-oil components. The addition of montmorillonite K10 to cellulose/polyethylene mixture promotes the deepest conversion of polyethylene and cellulose. Additionally, more saturated than unsaturated hydrocarbons are present in resultant bio-oils. The proportion of liquid hydrocarbons is the highest when a montmorillonite K10 is acting as a catalyst.« less

Biodiesel Production from Chlorella protothecoides Oil by Microwave-Assisted Transesterification

PubMed Central

Gülyurt, Mustafa Ömer; Özçimen, Didem; İnan, Benan

2016-01-01

In this study, biodiesel production from microalgal oil by microwave-assisted transesterification was carried out to investigate its efficiency. Transesterification reactions were performed by using Chlorella protothecoides oil as feedstock, methanol, and potassium hydroxide as the catalyst. Methanol:oil ratio, reaction time and catalyst:oil ratio were investigated as process parameters affected methyl ester yield. 9:1 methanol/oil molar ratio, 1.5% KOH catalyst/oil ratio and 10 min were optimum values for the highest fatty acid methyl ester yield. PMID:27110772

Biodiesel Production from Chlorella protothecoides Oil by Microwave-Assisted Transesterification.

PubMed

Gülyurt, Mustafa Ömer; Özçimen, Didem; İnan, Benan

2016-04-22

In this study, biodiesel production from microalgal oil by microwave-assisted transesterification was carried out to investigate its efficiency. Transesterification reactions were performed by using Chlorella protothecoides oil as feedstock, methanol, and potassium hydroxide as the catalyst. Methanol:oil ratio, reaction time and catalyst:oil ratio were investigated as process parameters affected methyl ester yield. 9:1 methanol/oil molar ratio, 1.5% KOH catalyst/oil ratio and 10 min were optimum values for the highest fatty acid methyl ester yield.

Design and manufacture a coconut milk squeezer

NASA Astrophysics Data System (ADS)

Wayan Surata, I.; Gde Tirta Nindhia, Tjokorda; Budyanto, D.; Yulianto, A. E.

2017-05-01

The process of cooking oil production generally is started by grating the ripe coconut meat, then pressing the grated meat to obtain coconut milk, and finally heating the coconut milk to obtain the cooking oil. Pressing mechanism to obtain coconut milk is a very important step and decisive in the process of producing cooking oil. The amount of milk produced depends on the pressure applied at the time of pressing grated coconut. The higher the pressure, the more milk is obtained. Some commercial mechanical pressing tools that available in the market are not efficient due to the working steps too much and take long time per cycle of work. The aims of this study was to design and manufacture a power screw squeezer for the collection of coconut milk. Power screw produces a compressive force in the cylinder to push and press the grated coconut until the end of the cylinder while the coconut milk and coconut dregs flow out simultaneously. Screw press was designed using straight shaft configuration with square profile. Performance test was done to investigate the actual capacity and yield of milk produced. The results showed that squeezer of grated coconut worked well with capacity an average of 13,63 kg/h and coconut milk yield of 58%.

Olive oil pilot-production assisted by pulsed electric field: impact on extraction yield, chemical parameters and sensory properties.

PubMed

Puértolas, Eduardo; Martínez de Marañón, Iñigo

2015-01-15

The impact of the use of pulsed electric field (PEF) technology on Arroniz olive oil production in terms of extraction yield and chemical and sensory quality has been studied at pilot scale in an industrial oil mill. The application of a PEF treatment (2 kV/cm; 11.25 kJ/kg) to the olive paste significantly increased the extraction yield by 13.3%, with respect to a control. Furthermore, olive oil obtained by PEF showed total phenolic content, total phytosterols and total tocopherols significantly higher than control (11.5%, 9.9% and 15.0%, respectively). The use of PEF had no negative effects on general chemical and sensory characteristics of the olive oil, maintaining the highest quality according to EU legal standards (EVOO; extra virgin olive oil). Therefore, PEF could be an appropriate technology to improve olive oil yield and produce EVOO enriched in human-health-related compounds, such as polyphenols, phytosterols and tocopherols. Copyright © 2014 Elsevier Ltd. All rights reserved.

Effect of jasmonic acid elicitation on the yield, chemical composition, and antioxidant and anti-inflammatory properties of essential oil of lettuce leaf basil (Ocimum basilicum L.).

PubMed

Złotek, Urszula; Michalak-Majewska, Monika; Szymanowska, Urszula

2016-12-15

The effect of elicitation with jasmonic acid (JA) on the plant yield, the production and composition of essential oils of lettuce leaf basil was evaluated. JA-elicitation slightly affected the yield of plants and significantly increased the amount of essential oils produced by basil - the highest oil yield (0.78±0.005mL/100gdw) was achieved in plants elicited with 100μM JA. The application of the tested elicitor also influenced the chemical composition of basil essential oils - 100μM JA increased the linalool, eugenol, and limonene levels, while 1μM JA caused the highest increase in the methyl eugenol content. Essential oils from JA-elicited basil (especially 1μM and 100μM) exhibited more effective antioxidant and anti-inflammatory potential; therefore, this inducer may be a very useful biochemical tool for improving production and composition of herbal essential oils. Copyright © 2016 Elsevier Ltd. All rights reserved.

Bio-oil production via fast pyrolysis of biomass residues from cassava plants in a fluidised-bed reactor.

PubMed

Pattiya, Adisak

2011-01-01

Biomass residues from cassava plants, namely cassava stalk and cassava rhizome, were pyrolysed in a fluidised-bed reactor for production of bio-oil. The aims of this work were to investigate the yields and properties of pyrolysis products produced from both feedstocks as well as to identify the optimum pyrolysis temperature for obtaining the highest organic bio-oil yields. Results showed that the maximum yields of the liquid bio-oils derived from the stalk and rhizome were 62 wt.% and 65 wt.% on dry basis, respectively. The pyrolysis temperatures that gave highest bio-oil yields for both feedstocks were in the range of 475-510 °C. According to the analysis of the bio-oils properties, the bio-oil derived from cassava rhizome showed better quality than that derived from cassava stalk as the former had lower oxygen content, higher heating value and better storage stability. Copyright © 2010 Elsevier Ltd. All rights reserved.

Application of response surface methodology to optimise supercritical carbon dioxide extraction of essential oil from Cyperus rotundus Linn.

PubMed

Wang, Hongwu; Liu, Yanqing; Wei, Shoulian; Yan, Zijun

2012-05-01

Supercritical fluid extraction with carbon dioxide (SC-CO2 extraction) was performed to isolate essential oils from the rhizomes of Cyperus rotundus Linn. Effects of temperature, pressure, extraction time, and CO2 flow rate on the yield of essential oils were investigated by response surface methodology (RSM). The oil yield was represented by a second-order polynomial model using central composite rotatable design (CCRD). The oil yield increased significantly with pressure (p<0.0001) and CO2 flow rate (p<0.01). The maximum oil yield from the response surface equation was predicted to be 1.82% using an extraction temperature of 37.6°C, pressure of 294.4bar, extraction time of 119.8 min, and CO2 flow rate of 20.9L/h. Copyright © 2011 Elsevier Ltd. All rights reserved.

Co-processing of olive bagasse with crude rapeseed oil via pyrolysis.

PubMed

Uçar, Suat; Karagöz, Selhan

2017-05-01

The co-pyrolysis of olive bagasse with crude rapeseed oil at different blend ratios was investigated at 500ºC in a fixed bed reactor. The effect of olive bagasse to crude rapeseed oil ratio on the product distributions and properties of the pyrolysis products were comparatively investigated. The addition of crude rapeseed oil into olive bagasse in the co-pyrolysis led to formation of upgraded biofuels in terms of liquid yields and properties. While the pyrolysis of olive bagasse produced a liquid yield of 52.5 wt %, the highest liquid yield of 73.5 wt % was obtained from the co-pyrolysis of olive bagasse with crude rapeseed oil at a blend ratio of 1:4. The bio-oil derived from olive bagasse contained 5% naphtha, 10% heavy naphtha, 30% gas oil, and 55% heavy gas oil. In the case of bio-oil obtained from the co-pyrolysis of olive bagasse with crude rapeseed oil at a blend ratio of 1:4, the light naphtha, heavy naphtha, and light gas oil content increased. This is an indication of the improved characteristics of the bio-oil obtained from the co-processing. The heating value of bio-oil from the pyrolysis of olive bagasse alone was 34.6 MJ kg -1 and the heating values of bio-oils obtained from the co-pyrolysis of olive bagasse with crude rapeseed oil ranged from 37.6 to 41.6 MJ kg -1 . It was demonstrated that the co-processing of waste biomass with crude plant oil is a good alternative to improve bio-oil yields and properties.

Effect of acid, steam explosion, and size reduction pretreatments on bio-oil production from sweetgum, switchgrass, and corn stover.

PubMed

Wang, Hui; Srinivasan, Radhakrishnan; Yu, Fei; Steele, Philip; Li, Qi; Mitchell, Brian; Samala, Aditya

2012-05-01

Bio-oil produced from biomass by fast pyrolysis has the potential to be a valuable substitute for fossil fuels. In a recent work on pinewood, we found that pretreatment alters the structure and chemical composition of biomass, which influence fast pyrolysis. In this study, we evaluated dilute acid, steam explosion, and size reduction pretreatments on sweetgum, switchgrass, and corn stover feedstocks. Bio-oils were produced from untreated and pretreated feedstocks in an auger reactor at 450 °C. The bio-oil's physical properties of pH, water content, acid value, density, and viscosity were measured. The chemical characteristics of the bio-oils were determined by gas chromatography-mass spectrometry. The results showed that bio-oil yield and composition were influenced by the pretreatment method and feedstock type. Bio-oil yields of 52, 33, and 35 wt% were obtained from medium-sized (0.68-1.532 mm) untreated sweetgum, switchgrass, and corn stover, respectively, which were higher than the yields from other sizes. Bio-oil yields of 56, 46, and 51 wt% were obtained from 1% H(2)SO(4)-treated medium-sized sweetgum, switchgrass, and corn stover, respectively, which were higher than the yields from untreated and steam explosion treatments.

Beneficial synergistic effect on bio-oil production from co-liquefaction of sewage sludge and lignocellulosic biomass.

PubMed

Leng, Lijian; Li, Jun; Yuan, Xingzhong; Li, Jingjing; Han, Pei; Hong, Yuchun; Wei, Feng; Zhou, Wenguang

2018-03-01

Co-liquefaction of municipal sewage sludge (MSS) and lignocellulosic biomass such as rice straw or wood sawdust at different mixing ratios and the characterization of the obtained bio-oil and bio-char were investigated. Synergistic effects were found during co-processing of MSS with biomass for production of bio-oil with higher yield and better fuel properties than those from individual feedstock. The co-liquefaction of MSS/rice straw (4/4, wt) increased the bio-oil yield from 22.74% (bio-oil yield from liquefaction of MSS individually) or 23.67% (rice straw) to 32.45%. Comparable increase on bio-oil yield was also observed for MSS/wood sawdust mixtures (2/6, wt). The bio-oils produced from MSS/biomass mixtures were mainly composed of esters and phenols with lower boiling points (degradation temperatures) than those from individual feedstock (identified with higher heavy bio-oil fractions). These synergistic effects were probably resulted from the interactions between the intermittent products of MSS and those of biomass during processing. Copyright © 2017 Elsevier Ltd. All rights reserved.

Catalytic hydroprocessing of fast pyrolysis oils: Impact of biomass feedstock on process efficiency

DOE PAGES

Carpenter, Daniel; Westover, Tyler; Howe, Daniel; ...

2016-12-01

Here, we report here on an experimental study to produce refinery-ready fuel blendstocks via catalytic hydrodeoxygenation (upgrading) of pyrolysis oil using several biomass feedstocks and various blends. Blends were tested along with the pure materials to determine the effect of blending on product yields and qualities. Within experimental error, oil yields from fast pyrolysis and upgrading are shown to be linear functions of the blend components. Switchgrass exhibited lower fast pyrolysis and upgrading yields than the woody samples, which included clean pine, oriented strand board (OSB), and a mix of pinon and juniper (PJ). The notable exception was PJ, formore » which the poor upgrading yield of 18% was likely associated with the very high viscosity of the PJ fast pyrolysis oil (947 cp). The highest fast pyrolysis yield (54% dry basis) was obtained from clean pine, while the highest upgrading yield (50%) was obtained from a blend of 80% clean pine and 20% OSB (CP 8OSB 2). For switchgrass, reducing the fast pyrolysis temperature to 450 degrees C resulted in a significant increase to the pyrolysis oil yield and reduced hydrogen consumption during hydrotreating, but did not directly affect the hydrotreating oil yield. The water content of fast pyrolysis oils was also observed to increase linearly with the summed content of potassium and sodium, ranging from 21% for clean pine to 37% for switchgrass. Multiple linear regression models demonstrate that fast pyrolysis is strongly dependent upon the contents lignin and volatile matter as well as the sum of potassium and sodium.« less

Improvement of biodiesel production by lipozyme TL IM-catalyzed methanolysis using response surface methodology and acyl migration enhancer.

PubMed

Wang, Y; Wu, H; Zong, M H

2008-10-01

The process of biodiesel production from corn oil catalyzed by lipozyme TL IM, an inexpensive 1,3-position specific lipase from Thermomyces lanuginosus was optimized by response surface methodology (RSM) and a central composite rotatable design (CCRD) was used to study the effects of enzyme dosage, ratio of t-butanol to oil (v/v) and ratio of methanol to oil (mol/mol) on the methyl esters (ME) yield of the methanolysis. The optimum combinations for the reaction were 25.9U/goil of enzyme, 0.58 volume ratio of t-butanol to oil and 0.5, 0.5, 2.8 molar equivalent of methanol to oil added at the reaction time of 0, 2, and 4h, respectively, by which a ME yield of 85.6%, which was very close to the predicted value of 85.0%, could be obtained after reaction for 12h. Waste oil was found to be more suitable feedstock, and could give 93.7% ME yield under the optimum conditions described above. Adding triethylamine (TEA), an acyl migration enhancer, could efficiently improve the ME yield of the methanolysis of corn oil, giving a ME yield of 92.0%.

The ultrasound-assisted aqueous extraction of rice bran oil.

PubMed

Khoei, Maryam; Chekin, Fereshteh

2016-03-01

In this work, aqueous extraction of rice bran oil was done without and with ultrasound pretreatment. Key factors controlling the extraction and optimal operating conditions were identified. The highest extraction efficiency was found at pH=12, temperature of 45°C, agitation speed of 800rpm and agitation time of 15min, ultrasound treatment time of 70min and ultrasound treatment temperature of 25°C. Moreover, extraction yields were compared to ultrasound-assisted aqueous extraction and Soxhlet extraction. The results showed that the yield of rice bran oil at ultrasound-assisted aqueous extraction was close to the yield of oil extracted by hexane Soxhlet extraction. This result implied that the yield of rice bran oil was significantly influenced by ultrasound. With regard to quality, the oil extracted by ultrasound-assisted aqueous process had a lower content of free fatty acid and lower color imparting components than the hexane-extracted oil. Also, effect of parboiling of paddy on hexane and ultrasound-assisted aqueous extraction was studied. Both extraction methods gives higher percentage of oil from par boiled rice bran compared with raw rice bran. This may be due to the fact that parboiling releases the oil. Copyright © 2015 Elsevier Ltd. All rights reserved.

Influence sample sizing of citrus hystrix essential oil from hydrodistillation extraction

NASA Astrophysics Data System (ADS)

Yahya, A.; Amadi, I.; Hashib, S. A.; Mustapha, F. A.

2018-03-01

Essential oil extracted from kaffir lime leaves through hydrodistillation. The objective of this study is to quantify the oil production rate by identify the significant influence of particle size on kaffir lime leaves. Kaffir lime leaves were ground and separated by using siever into 90, 150, 300 μm and other kaffir lime leaves. The mean essential oil yield of 0.87, 0.52, 0.41 and 0.3% was obtained. 90 μm of ground gives the highest yield compared to other sizes. Thus, it can be concluded that in quantifying oil production rate, the relevance of different size of particle is clearly affects the amount of oil yield. In analysing the composition of kaffir lime essential oil using GC-MS, there were 38 compounds found in the essential oil. Some of the major compounds of the kaffir lime leave oils were detected while some are not, may due to oil experience thermal degradation which consequently losing some significant compounds in controlled temperature.

Improvement of neutral oil quality in the production of sulfonate additives

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhurba, A.S.; Bludilin, V.M.; Antonov, V.N.

This paper is concerned with improvement of neutral oil used as materials for sulfonation to produce additives for lubricating oils. In this article the authors analyze the basic reasons for the unsatisfactory quality of the neutral oil and attempt to define the ways in which the process technology can be improved so as to produce neutral oil with the required composition, at the same time raising the efficiency of utilization of the MSG-8 oil used as a feedstock for this process. Experimental results are presented which demonstrate the feasibility of sulfonating neutral oil in the high-speed mixer under near-optimal conditions.more » The yield of sulfonic acid approaches the theoretical yield. With the lowest contents of aromatic hydrocarbons in the original neutral oil, the aromatic hydrocarbons are almost completely converted to sulfonic acids. The yield of neutral oil is sufficiently high, and the residual content of aromatic hydrocarbons in the oil is no greater than 3%.« less

Ball milling pretreatment of oil palm biomass for enhancing enzymatic hydrolysis.

PubMed

Zakaria, Mohd Rafein; Fujimoto, Shinji; Hirata, Satoshi; Hassan, Mohd Ali

2014-08-01

Oil palm biomass, namely empty fruit bunch and frond fiber, were pretreated using a planetary ball mill. Particle sizes and crystallinity index values of the oil palm biomass were significantly reduced with extended ball mill processing time. The treatment efficiency was evaluated by the generation of glucose, xylose, and total sugar conversion yields from the pretreatment process compared to the amount of sugars from raw materials. Glucose and xylose contents were determined using high-performance liquid chromatography. An increasing trend in glucose and xylose yield as well as total sugar conversion yield was observed with decreasing particle size and crystallinity index. Oil palm frond fiber exhibited the best material yields using ball milling pretreatment with generated glucose, xylose, and total sugar conversion yields of 87.0, 81.6, and 85.4%, respectively. In contrast, oil palm empty fruit bunch afforded glucose and xylose of 70.0 and 82.3%, respectively. The results obtained in this study showed that ball mill-treated oil palm biomass is a suitable pretreatment method for high conversion of glucose and xylose.

β-Sitosterol: supercritical carbon dioxide extraction from sea buckthorn (Hippophae rhamnoides L.) seeds.

PubMed

Sajfrtová, Marie; Licková, Ivana; Wimmerová, Martina; Sovová, Helena; Wimmer, Zdenek

2010-04-22

Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn (Hippophae rhamnoides L.) seeds were extracted with supercritical carbon dioxide at pressures ranging from 15-60 MPa and temperatures of 40-80 degrees C. Oil and β-sitosterol yields were measured in the extraction course and compared with Soxhlet extraction with hexane. The average yield of β-sitosterol was 0.31 mg/g of seeds. The maximum concentration of β-sitosterol in the extract, 0.5% w/w, was achieved at 15 MPa, 40 degrees C, and a carbon dioxide consumption of 50 g/g of seeds. The extraction rate was maximal at 60 MPa and 40 degrees C. Both β-sitosterol yield and its concentration in the extract obtained with hexane were lower than with carbon dioxide.

Modification of yield and composition of essential oils by distillation time

USDA-ARS?s Scientific Manuscript database

Field and laboratory experiments were conducted to model the length of the steam distillation time (DT) on essential oil yield and oil composition of peppermint, lemongrass, and palmarosa oils. The DTs tested were 1.25, 2.5, 5, 10, 20, 40, 80, and 160 min for peppermint, and 1.25, 2.5, 5, 10, 20, 40...

Oil palm mapping for Malaysia using PALSAR-2 dataset

NASA Astrophysics Data System (ADS)

Gong, P.; Qi, C. Y.; Yu, L.; Cracknell, A.

2016-12-01

Oil palm is one of the most productive vegetable oil crops in the world. The main oil palm producing areas are distributed in humid tropical areas such as Malaysia, Indonesia, Thailand, western and central Africa, northern South America, and central America. Increasing market demands, high yields and low production costs of palm oil are the primary factors driving large-scale commercial cultivation of oil palm, especially in Malaysia and Indonesia. Global demand for palm oil has grown exponentially during the last 50 years, and the expansion of oil palm plantations is linked directly to the deforestation of natural forests. Satellite remote sensing plays an important role in monitoring expansion of oil palm. However, optical remote sensing images are difficult to acquire in the Tropics because of the frequent occurrence of thick cloud cover. This problem has led to the use of data obtained by synthetic aperture radar (SAR), which is a sensor capable of all-day/all-weather observation for studies in the Tropics. In this study, the ALOS-2 (Advanced Land Observing Satellite) PALSAR-2 (Phased Array type L-band SAR) datasets for year 2015 were used as an input to a support vector machine (SVM) based machine learning algorithm. Oil palm/non-oil palm samples were collected using a hexagonal equal-area sampling design. High-resolution images in Google Earth and PALSAR-2 imagery were used in human photo-interpretation to separate oil palm from others (i.e. cropland, forest, grassland, shrubland, water, hard surface and bareland). The characteristics of oil palms from various aspects, including PALSAR-2 backscattering coefficients (HH, HV), terrain and climate by using this sample set were further explored to post-process the SVM output. The average accuracy of oil palm type is better than 80% in the final oil palm map for Malaysia.

Task 1: Whole-body concentrations of elements in kelp bass (Paralabrax clathratus), kelp rockfish (Sebastes atrovirens), and Pacific sanddab (Citharichthys sordidus) from offshore oil platforms and natural areas in the Southern California Bight

USGS Publications Warehouse

Love, Milton S.

2009-01-01

Resource managers are concerned that offshore oil platforms in the Southern California Bight may be contributing to environmental contaminants accumulated by marine fishes. To examine this possibility, 18 kelp bass (Paralabrax clathratus), 80 kelp rockfish (Sebastes atrovirens), and 98 Pacific sanddab (Citharichthys sordidus) were collected from five offshore oil platforms and 10 natural areas during 2005-2006 for whole-body analysis of 63 elements. The natural areas, which served as reference sites, were assumed to be relatively uninfluenced by contaminants originating from platforms. Forty-two elements were excluded from statistical comparisons for one of three reasons: they consisted of major cations that were unlikely to accumulate to potentially toxic concentrations under ambient exposure conditions; they were not detected by the analytical procedures; or they were detected at concentrations too low to yield reliable quantitative measurements. The remaining 21 elements consisted of aluminum, arsenic, barium, cadmium, chromium, cobalt, copper, gallium, iron, lead, lithium, manganese, mercury, nickel, rubidium, selenium, strontium, tin, titanium, vanadium, and zinc. Statistical comparisons of these 21 elements indicated that none consistently exhibited higher concentrations at oil platforms than at natural areas. Eight comparisons yielded significant interaction effects between total length (TL) of the fish and the two habitat types (oil platforms and natural areas). This indicated that relations between certain elemental concentrations (i.e., copper, rubidium, selenium, tin, titanium, and vanadium) and habitat type varied by TL of affected fish species. To better understand these interactions, we examined elemental concentrations in very small and very large individuals of affected species. Although significant interactions were detected for rubidium, tin, and selenium in kelp rockfish, the concentrations of these elements did not differ significantly between oil platforms and natural areas over the TL range of sampled fish. However, for selenium, titanium, and vanadium in Pacific sanddab, small individuals (average TL, 13.0 cm) exhibited significantly lower concentrations at oil platforms than at natural areas, whereas large individuals (average TL, 27.5 cm) exhibited higher concentrations at oil platforms than at natural areas. For copper in Pacific sanddab, small individuals did not exhibit differences between oil platforms and natural areas, whereas large individuals exhibited significantly higher concentrations at oil platforms than at natural areas. On the other hand, for tin in Pacific sanddab, small individuals did not exhibit differences between oil platforms and natural areas, whereas large individuals exhibited significantly lower concentrations at oil platforms than at natural areas. Although concentrations of arsenic, cadmium, chromium, lead, mercury, and selenium in fishes from some platforms and natural areas equaled or exceeded literature-based toxicity thresholds for fish and fish-eating wildlife, studies are still needed to document evidence of toxicity from these elements. When estimates of elemental concentrations in skinless fillets were compared to risk-based consumption limits for humans, the concentrations of arsenic, cadmium, mercury, and tin in fish from a mix of oil platforms and natural areas were sufficiently elevated to suggest a need for further study of inorganic arsenic, cadmium, mercury, and tributyltin.

Oleic Acid enhances all-trans retinoic Acid loading in nano-lipid emulsions.

PubMed

Chinsriwongkul, Akhayachatra; Opanasopit, Praneet; Ngawhirunpat, Tanasait; Rojanarata, Theerasak; Sila-On, Warisada; Ruktanonchai, Uracha

2010-01-01

The aim of this study was to investigate the enhancement of all-trans retinoic acid (ATRA) loading in nano-lipid emulsions and stability by using oleic acid. The effect of formulation factors including initial ATRA concentration and the type of oil on the physicochemical properties, that is, percentage yield, percentage drug release, and photostability of formulations, was determined. The solubility of ATRA was increased in the order of oleic acid > MCT > soybean oil > water. The physicochemical properties of ATRA-loaded lipid emulsion, including mean particle diameter and zeta potential, were modulated by changing an initial ATRA concentration as well as the type and mixing ratio of oil and oleic acid as an oil phase. The particles of lipid emulsions had average sizes of less than 250 nm and negative zeta potential. The addition of oleic acid in lipid emulsions resulted in high loading capacity. The photodegradation rate was found to be dependent on the initial drug concentration but independent of the type of oily phase used in this study. The release rates were not affected by initial ATRA concentration but were affected by the type of oil, where oleic acid showed the highest release rate of ATRA from lipid emulsions.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carpenter, Daniel; Westover, Tyler; Howe, Daniel

Here, we report here on an experimental study to produce refinery-ready fuel blendstocks via catalytic hydrodeoxygenation (upgrading) of pyrolysis oil using several biomass feedstocks and various blends. Blends were tested along with the pure materials to determine the effect of blending on product yields and qualities. Within experimental error, oil yields from fast pyrolysis and upgrading are shown to be linear functions of the blend components. Switchgrass exhibited lower fast pyrolysis and upgrading yields than the woody samples, which included clean pine, oriented strand board (OSB), and a mix of pinon and juniper (PJ). The notable exception was PJ, formore » which the poor upgrading yield of 18% was likely associated with the very high viscosity of the PJ fast pyrolysis oil (947 cp). The highest fast pyrolysis yield (54% dry basis) was obtained from clean pine, while the highest upgrading yield (50%) was obtained from a blend of 80% clean pine and 20% OSB (CP 8OSB 2). For switchgrass, reducing the fast pyrolysis temperature to 450 degrees C resulted in a significant increase to the pyrolysis oil yield and reduced hydrogen consumption during hydrotreating, but did not directly affect the hydrotreating oil yield. The water content of fast pyrolysis oils was also observed to increase linearly with the summed content of potassium and sodium, ranging from 21% for clean pine to 37% for switchgrass. Multiple linear regression models demonstrate that fast pyrolysis is strongly dependent upon the contents lignin and volatile matter as well as the sum of potassium and sodium.« less

ACC oxidase and miRNA 159a, and their involvement in fresh fruit bunch yield (FFB) via sex ratio determination in oil palm.

PubMed

Somyong, Suthasinee; Poopear, Supannee; Sunner, Supreet Kaur; Wanlayaporn, Kitti; Jomchai, Nukoon; Yoocha, Thippawan; Ukoskit, Kittipat; Tangphatsornruang, Sithichoke; Tragoonrung, Somvong

2016-06-01

Oil palm (Elaeis guineesis Jacq.) is the most productive oil-bearing crop, yielding more oil per area than any other oil-bearing crops. However, there are still efforts to improve oil palm yield, in order to serve consumer and manufacturer demand. Oil palm produces female and male inflorescences in an alternating cycle. So, high sex ratio (SR), the ratio of female inflorescences to the total inflorescences, is a favorable trait in term of increasing yields in oil palm. This study aims to understand the genetic control for SR related traits, such as fresh fruit bunch yield (FFB), by characterizing genes at FFB quantitative trait loci (QTLs) on linkage 10 (chromosome 6) and linkage 15 (chromosome 10). Published oil palm sequences at the FFB QTLs were used to develop gene-based and simple sequence repeat (SSR) markers. We used the multiple QTL analysis model (MQM) to characterize the relationship of new markers with the SR traits in the oil palm population. The RNA expression of the most linked QTL genes was also evaluated in various tissues of oil palm. We identified EgACCO1 (encoding aminocyclopropane carboxylate (ACC) oxidase) at chromosome 10 and EgmiR159a (microRNA 159a) at chromosome 6 to be the most linked QTL genes or determinants for FFB yield and/or female inflorescence number with a phenotype variance explained (PVE) from 10.4 to 15 % and suggest that these play the important roles in sex determination and differentiation in oil palm. The strongest expression of EgACCO1 and the predicted precursor of EgmiR159a was found in ovaries and, to a lesser extent, fruit development. In addition, highly normalized expression of EgmiR159a was found in female flowers. In summary, the QTL analysis and the RNA expression reveal that EgACCO1 and EgmiR159a are the potential genetic factors involved in female flower determination and hence would affect yield in oil palm. However, to clarify how these genetic factors regulate female flower determination, more investigation of their down regulation or target may be essential. Additionally, if more sex determination genes controlled by plant hormones are identified, it may possible to reveal a crosstalk of sex determination genes with hormones and environment factors.

Effect of drying on yield and calorific values of extractables from leafy spurge (Euphorbia esula)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wiatr, S.M.

1984-04-01

The effect of dehydration on yield and calorific values has been investigated for oils, hydrocarbons, and poly-phenols extracted from leaves of Euphorbia esula (leafy spurge). Methods of dehydration employed were with a warm oven (50 degrees C), a hot oven (105 degrees C), at room temperature (25 degrees C), and with freeze drying. Generally, dehydration resulted in a loss of yield for all extractives. Noteworthy exceptions were oil yields from the warm-oven or air-dried biomass which did not differ significantly from fresh tissue. Significant differences in calorific values (range 4643-5192 cal/g) were observed for each category of whole leafy biomassmore » dehydrated as indicated above. Dehydration reduced the calorific value of oils (range 9483-10,095 cal/g) but tended to increase the calorific values of polyphenols (range 4178-6033 cal/g). NMR spectroscopy of the oil fraction suggested that dehydration did not grossly alter the composition of oils, despite differences in yield and calorific values.« less

Effect of drying on yield and calorific values of extractables from leafy spurge (Euphorbia esula)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wiatr, S.M.

1984-01-01

The effect of dehydration on yield and calorific values has been investigated for oils, hydrocarbons, and polyphenols extracted from leaves of Euphorbia esula (leafy spurge). Methods of dehydration employed were with a warm oven (50/sup 0/C), a hot oven (105/sup 0/C), at room temperature (25/sup 0/C), and with freeze drying. Generally, dehydration resulted in a loss of yield for all extractives. Noteworthy exceptions were oil yields from the warm-oven- or air-dried biomass which did not differ significantly from fresh tissue. Significant differences in calorific values (range 4643-5192 cal/g) were observed for each category of whole leafy biomass dehydrated as indicatedmore » above. Dehydration reduced the calorific value of oils (range 9483-10,095 cal/g) but tended to increase the calorific values of polyphenols (range 4178-6033 cal/g). NMR spectroscopy of the oil fraction suggested that dehydration did not grossly alter the composition of oils, despite differences in yield and calorific values.« less

Biomass fast pyrolysis for bio-oil production in a fluidized bed reactor under hot flue atmosphere.

PubMed

Li, Ning; Wang, Xiang; Bai, Xueyuan; Li, Zhihe; Zhang, Ying

2015-10-01

Fast pyrolysis experiments of corn stalk were performed to investigate the optimal pyrolysis conditions of temperature and bed material for maximum bio-oil production under flue gas atmosphere. Under the optimized pyrolysis conditions, furfural residue, xylose residue and kelp seaweed were pyrolyzed to examine their yield distributions of products, and the physical characteristics of bio-oil were studied. The best flow rate of the flue gas at selected temperature is obtained, and the pyrolysis temperature at 500 degrees C and dolomite as bed material could give a maximum bio-oil yield. The highest bio-oil yield of 43.3% (W/W) was achieved from corn stalk under the optimal conditions. Two main fractions were recovered from the stratified bio-oils: light oils and heavy oils. The physical properties of heavy oils from all feedstocks varied little. The calorific values of heavy oils were much higher than that of light oils. The pyrolysis gas could be used as a gaseous fuel due to a relatively high calorific value of 6.5-8.5 MJ/m3.

Hydrothermal liquefaction of separated dairy manure for production of bio-oils with simultaneous waste treatment.

PubMed

Theegala, Chandra S; Midgett, Jason S

2012-03-01

A bench scale hydrothermal liquefaction (HTL) system was tested using dairy manure to explore biooil production and waste treatment potential. Carbon monoxide was used as the process gas and sodium carbonate (Na(2)CO(3)) as catalyst. At a 350°C process temperature, the HTL unit produced 3.45 g (± 0.21) of acetone soluble oil fractions (ASF), with an average Higher Heating Value of 32.16 (± 0.23) MJ kg(-1). A maximum ASF yield of 4.8 g was produced at a process temperature of 350°C and 1g of catalyst. The best ASF yield corresponded to 67.6% of energy contained in the raw manure. GC-MS analysis of ASF indicated that the highest quantities of phenolic compounds were formed when 1g catalyst was used. Chemical Oxygen Demand (COD) reduction in the dischargeable slurry was as high as 75%. The results point to an alternative dairy waste treatment technology with a potential to generate transportable biooils. Copyright © 2011 Elsevier Ltd. All rights reserved.

Study of quantitative and qualitative variations in essential oils of Sicilian Rosmarinus officinalis L.

PubMed

Tuttolomondo, Teresa; Dugo, Giacomo; Ruberto, Giuseppe; Leto, Claudio; Napoli, Edoardo M; Cicero, Nicola; Gervasi, Teresa; Virga, Giuseppe; Leone, Raffaele; Licata, Mario; La Bella, Salvatore

2015-01-01

In this study the chemical characterisation of 10 Sicilian Rosmarinus officinalis L. biotypes essential oils is reported. The main goal of this work was to analyse the relationship between the essential oils yield and the geographical distribution of the species plants. The essential oils were analysed by GC-FID and GC-MS. Hierarchical cluster analysis and principal component analysis statistical methods were used to cluster biotypes according to the essential oils chemical composition. The essential oil yield ranged from 0.8 to 2.3 (v/w). In total 82 compounds have been identified, these represent 96.7-99.9% of the essential oil. The most represented compounds in the essential oils were 1.8-cineole, linalool, α-terpineol, verbenone, α-pinene, limonene, bornyl acetate and terpinolene. The results show that the essential oil yield of the 10 biotypes is affected by the environmental characteristics of the sampling sites while the chemical composition is linked to the genetic characteristics of different biotypes.

Effect of unsaturated fatty acids and triglycerides from soybeans on milk fat synthesis and biohydrogenation intermediates in dairy cattle.

PubMed

Boerman, J P; Lock, A L

2014-11-01

Increased rumen unsaturated fatty acid (FA) load is a risk factor for milk fat depression. This study evaluated if increasing the amount of unsaturated FA in the diet as triglycerides or free FA affected feed intake, yield of milk and milk components, and feed efficiency. Eighteen Holstein cows (132 ± 75 d in milk) were used in a replicated 3 × 3 Latin square design. Treatments were a control (CON) diet, or 1 of 2 unsaturated FA (UFA) treatments supplemented with either soybean oil (FA present as triglycerides; TAG treatment) or soybean FA distillate (FA present as free FA; FFA treatment). The soybean oil contained a higher concentration of cis-9 C18:1 (26.0 vs. 11.8 g/100g of FA) and lower concentrations of C16:0 (9.6 vs. 15.0 g/100g of FA) and cis-9,cis-12 C18:2 (50.5 vs. 59.1g/100g of FA) than the soybean FA distillate. The soybean oil and soybean FA distillate were included in the diet at 2% dry matter (DM) to replace soyhulls in the CON diet. Treatment periods were 21 d, with the final 4 d used for sample and data collection. The corn silage- and alfalfa silage-based diets contained 23% forage neutral detergent fiber and 17% crude protein. Total dietary FA were 2.6, 4.2, and 4.3% of diet DM for CON, FFA, and TAG treatments, respectively. Total FA intake was increased 57% for UFA treatments and was similar between FFA and TAG. The intakes of individual FA were similar, with the exception of a 24 g/d lower intake of C16:0 and a 64 g/d greater intake of cis-9 C18:1 for the TAG compared with the FFA treatment. Compared with CON, the UFA treatments decreased DM intake (1.0 kg/d) but increased milk yield (2.2 kg/d) and milk lactose concentration and yield. The UFA treatments reduced milk fat concentration, averaging 3.30, 3.18, and 3.11% for CON, FFA, and TAG treatments, respectively. Yield of milk fat, milk protein, and 3.5% fat-corrected milk remained unchanged when comparing CON with the UFA treatments. No differences existed in the yield of milk or milk components between the FFA and TAG treatments. The UFA treatments increased feed efficiency (energy-corrected milk/DM intake), averaging 1.42, 1.53, and 1.48 for CON, FFA, and TAG treatments, respectively. Although milk fat yield was not affected, the UFA treatments decreased the yield of de novo (<16-carbon) synthesized FA (40 g/d) and increased the yield of preformed (>16-carbon) FA (134 g/d). Yield of FA from both sources (16-carbon FA) was reduced by the UFA treatments but to a different extent for FFA versus TAG (72 vs. 100g/d). An increase was detected in the concentration of trans-10 C18:1 and a trend for an increase in trans-10,cis-12 C18:2 and trans-9,cis-11 C18:2 for the UFA treatments compared with CON. Under the dietary conditions tested, UFA treatments supplemented at 2% diet DM as either soybean FA distillate or soybean oil increased milk yield but did not effectively cause a reduction in milk fat yield, with preformed FA replacing de novo synthesized FA in milk fat. Further research is required to determine if the response to changes in dietary free and esterified FA concentrations is different in diets that differ in their risk for milk fat depression. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Economic incentive for applying vetiver grass to remediate lead, copper and zinc contaminated soils.

PubMed

Danh, Luu Thai; Truong, Paul; Mammucari, Raffaella; Fostert, Neil

2011-01-01

The application of vetiver grass (Chrysopogon zizaniodes) for phytoremediation of heavy metal contaminated soils can be promoted by economic return through essential oil production. Four levels of lead (0, 500, 2000, and 8000 mg kg(-1) dry soil), copper (0, 100, 400, and 1600 mg kg(-1) dry soil) and zinc (0, 400, 1600, and 6400 mg kg(-1) dry soil) were used to study their effects on vetiver growth, essential oil composition and yield. This study also investigated the effect of nitrogen concentrations on vetiver oil yield. Vetiver accumulated high concentrations of Pb, Cu and Zn in roots (3246, 754 and 2666 mg kg(-1), respectively) and small amounts of contaminants in shoots (327, 55, and 642 mg kg(-1), respectively). Oil content and yield were not affected at low and moderate concentrations of Cu and Zn. Only the application of Pb had a significant detrimental effect on oil composition. Extraction of vetiver essential oils by hydrodistillation produced heavy metal free products. High level of nitrogen reduced oil yields. Results show that phytoremediation of Cu and Zn contaminated soils by vetiver can generate revenue from the commercialization of oil extracts.

Fluidized-bed pyrolysis of oil shale: oil yield, composition, and kinetics

DOE Office of Scientific and Technical Information (OSTI.GOV)

Richardson, J H; Huss, E B; Ott, L L

1982-09-01

A quartz isothermal fluidized-bed reactor has been used to measure kinetics and oil properties relevant to surface processing of oil shale. The rate of oil formation has been described with two sequential first-order rate equations characterized by two rate constants, k/sub 1/ = 2.18 x 10/sup 10/ exp(-41.6 kcal/RT) s/sup -1/ and k/sub 2/ = 4.4 x 10/sup 6/ exp(-29.7 kcal/RT) s/sup -1/. These rate constants together with an expression for the appropriate weighting coefficients describe approximately 97/sup +/% of the total oil produced. A description is given of the results of different attempts to mathematically describe the data inmore » a manner suitable for modeling applications. Preliminary results are also presented for species-selective kinetics of methane, ethene, ethane and hydrogen, where the latter is clearly distinguished as the product of a distinct intermediate. Oil yields from Western oil shale are approximately 100% Fischer assay. Oil composition is as expected based on previous work and the higher heating rates (temperatures) inherent in fluidized-bed pyrolysis. Neither the oil yield, composition nor the kinetics varied with particle size between 0.2 and 2.0 mm within experimental error. The qualitatively expected change in oil composition due to cracking was observed over the temperature range studied (460 to 540/sup 0/C). Eastern shale exhibited significantly faster kinetics and higher oil yields than did Western shale.« less

Identification of Rubisco rbcL and rbcS in Camellia oleifera and their potential as molecular markers for selection of high tea oil cultivars.

PubMed

Chen, Yongzhong; Wang, Baoming; Chen, Jianjun; Wang, Xiangnan; Wang, Rui; Peng, Shaofeng; Chen, Longsheng; Ma, Li; Luo, Jian

2015-01-01

Tea oil derived from seeds of Camellia oleifera Abel. is high-quality edible oil in China. This study isolated full-length cDNAs of Rubisco subunits rbcL and rbcS from C. oleifera. The rbcL has 1,522 bp with a 1,425 bp coding region, encoding 475 amino acids; and the rbcS has 615 bp containing a 528 bp coding region, encoding 176 amino acids. The expression level of the two genes, designated as Co-rbcL and Co-rbcS, was determined in three C. oleifera cultivars: Hengchong 89, Xianglin 1, and Xianglin 14 whose annual oil yields were 546.9, 591.4, and 657.7 kg ha(-1), respectively. The Co-rbcL expression in 'Xianglin 14' was significantly higher than 'Xianglin 1', and 'Xianglin 1' was greater than 'Hengchong 89'. The expression levels of Co-rbcS in 'Xianglin 1' and 'Xianglin 14' were similar but were significantly greater than in 'Hengchong 89'. The net photosynthetic rate of 'Xianglin 14' was significantly higher than 'Xianglin 1', and 'Xianglin 1' was higher than 'Hengchong 89'. Pearson's correlation analysis showed that seed yields and oil yields were highly correlated with the expression level of Co-rbcL at P < 0.001 level; and the expression of Co-rbcS was correlated with oil yield at P < 0.01 level. Net photosynthetic rate was also correlated with oil yields and seed yields at P < 0.001 and P < 0.01 levels, respectively. Our results suggest that Co-rbcS and Co-rbcL in particular could potentially be molecular markers for early selection of high oil yield cultivars. In combination with the measurement of net photosynthetic rates, the early identification of potential high oil production cultivars would significantly shorten plant breeding time and increase breeding efficiency.

Using the candidate gene approach for detecting genes underlying seed oil concentration and yield in soybean.

PubMed

Eskandari, Mehrzad; Cober, Elroy R; Rajcan, Istvan

2013-07-01

Increasing the oil concentration in soybean seeds has been given more attention in recent years because of demand for both edible oil and biodiesel production. Oil concentration in soybean is a complex quantitative trait regulated by many genes as well as environmental conditions. To identify genes governing seed oil concentration in soybean, 16 putative candidate genes of three important gene families (GPAT: acyl-CoA:sn-glycerol-3-phosphate acyltransferase, DGAT: acyl-CoA:diacylglycerol acyltransferase, and PDAT: phospholipid:diacylglycerol acyltransferase) involved in triacylglycerol (TAG) biosynthesis pathways were selected and their sequences retrieved from the soybean database ( http://www.phytozome.net/soybean ). Three sequence mutations were discovered in either coding or noncoding regions of three DGAT soybean isoforms when comparing the parents of a 203 recombinant inbreed line (RIL) population; OAC Wallace and OAC Glencoe. The RIL population was used to study the effects of these mutations on seed oil concentration and other important agronomic and seed composition traits, including seed yield and protein concentration across three field locations in Ontario, Canada, in 2009 and 2010. An insertion/deletion (indel) mutation in the GmDGAT2B gene in OAC Wallace was significantly associated with reduced seed oil concentration across three environments and reduced seed yield at Woodstock in 2010. A mutation in the 3' untranslated (3'UTR) region of GmDGAT2C was associated with seed yield at Woodstock in 2009. A mutation in the intronic region of GmDGAR1B was associated with seed yield and protein concentration at Ottawa in 2010. The genes identified in this study had minor effects on either seed yield or oil concentration, which was in agreement with the quantitative nature of the traits. However, the novel gene-specific markers designed in the present study can be used in soybean breeding for marker-assisted selection aimed at increasing seed yield and oil concentration with no significant impact on seed protein concentration.

Dry column-thermal energy analyzer method for determining N-nitrosopyrrolidine in fried bacon: collaborative study.

PubMed

Fiddler, W; Pensabene, J W; Gates, R A; Phillips, J G

1984-01-01

A dry column method for isolating N-nitrosopyrrolidine (NPYR) from fried, cure-pumped bacon and detection by gas chromatography-thermal energy analyzer (TEA) was studied collaboratively. Testing the results obtained from 11 collaborators for homogeneous variances among samples resulted in splitting the nonzero samples into 2 groups of sample levels, each with similar variances. Outlying results were identified by AOAC-recommended procedures, and laboratories having outliers within a group were excluded. Results from the 9 collaborators remaining in the low group yielded coefficients of variation (CV) of 6.00% and 7.47% for repeatability and reproducibility, respectively, and the 8 collaborators remaining in the high group yielded CV values of 5.64% and 13.72%, respectively. An 85.2% overall average recovery of the N-nitrosoazetidine internal standard was obtained with an average laboratory CV of 10.5%. The method has been adopted official first action as an alternative to the mineral oil distillation-TEA screening procedure.

Meta-analyses of oil yield in Cuphea PSR23

USDA-ARS?s Scientific Manuscript database

Oil content and composition of Cuphea seed are of special economic value as raw materials for industrial and food applications. The inherent unpredictability in determining and predicting Cuphea’s oil yield is attributed, in part, to the indeterminate growth habit and the persistence of the domestic...

Deconstruction of lignocellulosic biomass with hydrated cerium (III) chloride in water and ethanol

DOE PAGES

Akalin, Mehmet K.; Das, Parthapratim; Alper, Koray; ...

2017-08-08

Lignocellulosic biomass was decomposed to produce crude bio-oil in water and ethanol using hydrated cerium (III) chloride as a catalyst. Use of the catalyst affected not only the yield of crude bio-oil but also the composition of bio-crude for both water and ethanol. The catalyst had a detrimental effect on the crude bio-oil yields obtained from water processing for all runs. However, in ethanol, use of the catalyst improved the crude bio-oil yields in all tested runs. The solid residue yields decreased with the catalyst use in the runs with water but increased in all studies with ethanol, except thosemore » with the shortest tested residence time of 10 min. The highest crude bio-oil yield of 48.2 wt% was obtained at 300 °C using 5 mmol of hydrated cerium (III) chloride at a residence time of 90 min in ethanol. The heating values of the crude bio-oils increased with the catalyst use for both water and ethanol processing. In conclusion, the highest heating value of 33.3 MJ kg –1 was obtained with hydrated cerium (III) chloride at 300 °C and a residence time of 120 min.« less

Deconstruction of lignocellulosic biomass with hydrated cerium (III) chloride in water and ethanol

DOE Office of Scientific and Technical Information (OSTI.GOV)

Akalin, Mehmet K.; Das, Parthapratim; Alper, Koray

Lignocellulosic biomass was decomposed to produce crude bio-oil in water and ethanol using hydrated cerium (III) chloride as a catalyst. Use of the catalyst affected not only the yield of crude bio-oil but also the composition of bio-crude for both water and ethanol. The catalyst had a detrimental effect on the crude bio-oil yields obtained from water processing for all runs. However, in ethanol, use of the catalyst improved the crude bio-oil yields in all tested runs. The solid residue yields decreased with the catalyst use in the runs with water but increased in all studies with ethanol, except thosemore » with the shortest tested residence time of 10 min. The highest crude bio-oil yield of 48.2 wt% was obtained at 300 °C using 5 mmol of hydrated cerium (III) chloride at a residence time of 90 min in ethanol. The heating values of the crude bio-oils increased with the catalyst use for both water and ethanol processing. In conclusion, the highest heating value of 33.3 MJ kg –1 was obtained with hydrated cerium (III) chloride at 300 °C and a residence time of 120 min.« less

Bio-oil production from fast pyrolysis of waste furniture sawdust in a fluidized bed.

PubMed

Heo, Hyeon Su; Park, Hyun Ju; Park, Young-Kwon; Ryu, Changkook; Suh, Dong Jin; Suh, Young-Woong; Yim, Jin-Heong; Kim, Seung-Soo

2010-01-01

The amount of waste furniture generated in Korea was over 2.4 million tons in the past 3 years, which can be used for renewable energy or fuel feedstock production. Fast pyrolysis is available for thermo-chemical conversion of the waste wood mostly into bio-oil. In this work, fast pyrolysis of waste furniture sawdust was investigated under various reaction conditions (pyrolysis temperature, particle size, feed rate and flow rate of fluidizing medium) in a fluidized-bed reactor. The optimal pyrolysis temperature for increased yields of bio-oil was 450 degrees C. Excessively smaller or larger feed size negatively affected the production of bio-oil. Higher flow and feeding rates were more effective for the production of bio-oil, but did not greatly affect the bio-oil yields within the tested ranges. The use of product gas as the fluidizing medium had a potential for increased bio-oil yields.

Supercritical Carbon Dioxide Extraction of the Oak Silkworm (Antheraea pernyi) Pupal Oil: Process Optimization and Composition Determination

PubMed Central

Pan, Wen-Juan; Liao, Ai-Mei; Zhang, Jian-Guo; Dong, Zeng; Wei, Zhao-Jun

2012-01-01

Supercritical carbon dioxide (SC-CO2) extraction of oil from oak silkworm pupae was performed in the present research. Response surface methodology (RSM) was applied to optimize the parameters of SC-CO2 extraction, including extraction pressure, temperature, time and CO2 flow rate on the yield of oak silkworm pupal oil (OSPO). The optimal extraction condition for oil yield within the experimental range of the variables researched was at 28.03 MPa, 1.83 h, 35.31 °C and 20.26 L/h as flow rate of CO2. Under this condition, the oil yield was predicted to be 26.18%. The oak silkworm pupal oil contains eight fatty acids, and is rich in unsaturated fatty acids and α-linolenic acid (ALA), accounting for 77.29% and 34.27% in the total oil respectively. PMID:22408458

Engineering Camelina sativa (L.) Crantz for enhanced oil and seed yields by combining diacylglycerol acyltransferase1 and glycerol-3-phosphate dehydrogenase expression.

PubMed

Chhikara, Sudesh; Abdullah, Hesham M; Akbari, Parisa; Schnell, Danny; Dhankher, Om Parkash

2018-05-01

Plant seed oil-based liquid transportation fuels (i.e., biodiesel and green diesel) have tremendous potential as environmentally, economically and technologically feasible alternatives to petroleum-derived fuels. Due to their nutritional and industrial importance, one of the major objectives is to increase the seed yield and oil production of oilseed crops via biotechnological approaches. Camelina sativa, an emerging oilseed crop, has been proposed as an ideal crop for biodiesel and bioproduct applications. Further increase in seed oil yield by increasing the flux of carbon from increased photosynthesis into triacylglycerol (TAG) synthesis will make this crop more profitable. To increase the oil yield, we engineered Camelina by co-expressing the Arabidopsis thaliana (L.) Heynh. diacylglycerol acyltransferase1 (DGAT1) and a yeast cytosolic glycerol-3-phosphate dehydrogenase (GPD1) genes under the control of seed-specific promoters. Plants co-expressing DGAT1 and GPD1 exhibited up to 13% higher seed oil content and up to 52% increase in seed mass compared to wild-type plants. Further, DGAT1- and GDP1-co-expressing lines showed significantly higher seed and oil yields on a dry weight basis than the wild-type controls or plants expressing DGAT1 and GPD1 alone. The oil harvest index (g oil per g total dry matter) for DGTA1- and GPD1-co-expressing lines was almost twofold higher as compared to wild type and the lines expressing DGAT1 and GPD1 alone. Therefore, combining the overexpression of TAG biosynthetic genes, DGAT1 and GPD1, appears to be a positive strategy to achieve a synergistic effect on the flux through the TAG synthesis pathway, and thereby further increase the oil yield. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

Lipase-catalyzed biodiesel production and quality with Jatropha curcas oil: exploring its potential for Central America.

PubMed

Bueso, Francisco; Moreno, Luis; Cedeño, Mathew; Manzanarez, Karla

2015-01-01

Extensive native Jatropha curcas L. (Jatropha) crop areas have been planted in Central America marginal lands since 2008 as a non-edible prospective feedstock alternative to high-value, edible palm oil. Jatropha biodiesel is currently exclusively produced in the region at commercial scale utilizing alkaline catalysts. Recently, a free, soluble Thermomyces lanuginosus (TL) 1,3 specific lipase has shown promise as biocatalyst, reportedly yielding up to 96 % ASTM D6751 compliant biodiesel after 24 h transesterification of soybean, canola oils and other feedstocks. Biodiesel conversion rate and quality of enzymatically catalyzed transesterification of Jatropha oil was evaluated. Two lipases: free, soluble TL and immobilized Candida antarctica (CA) catalyzed methanolic transesterification of crude Jatropha and refined palm oil. Jatropha yields were similar to palm biodiesel with NaOH as catalyst. After 24 h transesterification, Jatropha (81 %) and palm oil (86 %) biodiesel yields with TL as catalyst were significantly higher than CA (<70 %) but inferior to NaOH (>90 %). Enzymatic catalysts (TL and CA) produced Jatropha biodiesel with optimum flow properties but did not complied with ASTM D6751 stability parameters (free fatty acid content and oil stability index). Biodiesel production with filtered, degummed, low FFA Jatropha oil using a free liquid lipase (TL) as catalyst showed higher yielding potential than immobilized CA lipase as substitute of RBD palm oil with alkaline catalyst. However, Jatropha enzymatic biodiesel yield and stability were inferior to alkaline catalyzed biodiesel and not in compliance with international quality standards. Lower quality due to incomplete alcoholysis and esterification, potential added costs due to need of more than 24 h to achieve comparable biodiesel yields and extra post-transesterification refining reactions are among the remaining drawbacks for the environmentally friendlier enzymatic catalysis of crude Jatropha oil to become an economically viable alternative to chemical catalysis.

Simultaneous treatment of raw palm oil mill effluent and biodegradation of palm fiber in a high-rate CSTR.

PubMed

Khemkhao, Maneerat; Techkarnjanaruk, Somkiet; Phalakornkule, Chantaraporn

2015-02-01

A high-rate continuous stirred tank reactor (CSTR) was used to produce biogas from raw palm oil mill effluent (POME) at 55°C at a highest organic loading rate (OLR) of 19 g COD/ld. Physical and chemical pretreatments were not performed on the raw POME. In order to promote retention of suspended solids, the CSTR was installed with a deflector at its upper section. The average methane yield was 0.27 l/g COD, and the biogas production rate per reactor volume was 6.23 l/l d, and the tCOD removal efficiency was 82%. The hydrolysis rate of cellulose, hemicelluloses and lignin was 6.7, 3.0 and 1.9 g/d, respectively. The results of denaturing gradient gel electrophoresis (DGGE) suggested that the dominant hydrolytic bacteria responsible for the biodegradation of the palm fiber and residual oil were Clostridium sp., while the dominant methanogens were Methanothermobacter sp. Copyright © 2014 Elsevier Ltd. All rights reserved.

Biodiesel production using waste frying oil

DOE Office of Scientific and Technical Information (OSTI.GOV)

Charpe, Trupti W.; Rathod, Virendra K., E-mail: vk.rathod@ictmumbai.edu.in

2011-01-15

Research highlights: {yields} Waste sunflower frying oil is successfully converted to biodiesel using lipase as catalyst. {yields} Various process parameters that affects the conversion of transesterification reaction such as temperature, enzyme concentration, methanol: oil ratio and solvent are optimized. {yields} Inhibitory effect of methanol on lipase is reduced by adding methanol in three stages. {yields} Polar solvents like n-hexane and n-heptane increases the conversion of tranesterification reaction. - Abstract: Waste sunflower frying oil is used in biodiesel production by transesterification using an enzyme as a catalyst in a batch reactor. Various microbial lipases have been used in transesterification reaction tomore » select an optimum lipase. The effects of various parameters such as temperature, methanol:oil ratio, enzyme concentration and solvent on the conversion of methyl ester have been studied. The Pseudomonas fluorescens enzyme yielded the highest conversion. Using the P. fluorescens enzyme, the optimum conditions included a temperature of 45 deg. C, an enzyme concentration of 5% and a methanol:oil molar ratio 3:1. To avoid an inhibitory effect, the addition of methanol was performed in three stages. The conversion obtained after 24 h of reaction increased from 55.8% to 63.84% because of the stage-wise addition of methanol. The addition of a non-polar solvent result in a higher conversion compared to polar solvents. Transesterification of waste sunflower frying oil under the optimum conditions and single-stage methanol addition was compared to the refined sunflower oil.« less

Silane Modification of Glass and Silica Surfaces to Obtain Equally Oil-Wet Surfaces in Glass-Covered Silicon Micromodel Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grate, Jay W.; Warner, Marvin G.; Pittman, Jonathan W.

2013-08-05

The wettability of silicon and glass surfaces can be modified by silanization. However, similar treatments of glass and silica surfaces using the same silane do not necessarily yield the same wettability as determined by the oil-water contact angle. In this technical note, surface cleaning pretreatments were investigated to determine conditions that would yield oil-wet surfaces on glass with similar wettability to silica surfaces treated with the same silane, and both air-water and oil-water contact angles were determined. Air-water contact angles were less sensitive to differences between silanized silica and glass surfaces, often yielding similar values while the oil-water contact anglesmore » were quite different. Borosilicate glass surfaces cleaned with standard cleaning solution 1 (SC1) yield intermediate-wet surfaces when silanized with hexamethyldisilazane, while the same cleaning and silanization yields oil-wet surfaces on silica. However, cleaning glass in boiling concentrated nitric acid creates a surface that can be silanized to obtain oil-wet surfaces using HDMS. Moreover, this method is effective on glass with prior thermal treatment at an elevated temperature of 400oC. In this way, silica and glass can be silanized to obtain equally oil-wet surfaces using HMDS. It is demonstrated that pretreatment and silanization is feasible in silicon-silica/glass micromodels previously assembled by anodic bonding, and that the change in wettability has a significant observable effect on immiscisble fluid displacements in the pore network.« less

Seed oil extraction from red prickly pear using hexane and supercritical CO2 : assessment of phenolic compound composition, antioxidant and antibacterial activities.

PubMed

Koubaa, Mohamed; Mhemdi, Houcine; Barba, Francisco J; Angelotti, Armel; Bouaziz, Fatma; Chaabouni, Semia Ellouz; Vorobiev, Eugène

2017-01-01

Investigating Opuntia species for their seed oil content is of much importance owing to their potential use for food and in cosmetic applications. These oils have an important content in unsaturated fatty acids as well as antioxidant compounds (e.g. polyphenols, vitamin E), which have been associated with the prevention of some chronic diseases. Moreover, Opuntia stricta oils possess important antimicrobial activities. For instance, the main focus of this study was to compare the effectiveness of conventional (hexane extraction) and novel (supercritical (SC)-CO 2 ) extraction methods for the recovery of oil and phenolic compounds from O. stricta seeds. The oil yield of both extracts was then compared and the polyphenol content and composition of both extracts were determined by liquid chromatography-high-resolution mass spectrometry. Additionally, antioxidant (DPPH assay) and antimicrobial activities (disc diffusion method) of O. stricta seed oils were determined. The oil yield (based on Soxhlet's method) of O. stricta seeds was determined using SC-CO 2 (49.9 ± 2.2%), and hexane (49.0 ± 1.5%). Although obtaining similar oil extraction yields using the two methods, the extracted oil using SC-CO 2 was more enriched in polyphenols (172.2 ± 11.9 µg gallic acid equivalents (GAE) g -1 oil) than that extracted using hexane (76.0 ± 6.9 µg GAE g -1 of oil). Polyphenol profiles showed that the SC-CO 2 process led to the yield of more compounds (45) than that using hexane extraction (11). Moreover, the antioxidant and antimicrobial activities of SC-CO 2 extract showed a high percentage of inhibition. SC-CO 2 extraction of O. stricta seed oil led to extraction of oil with a similar yield to that with hexane extraction, but with higher polyphenol content. The extract containing polyphenols exhibited high antioxidant and antibacterial properties, demonstrating their great potential as feedstock for high-oil quality. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

β-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds

PubMed Central

Sajfrtová, Marie; Ličková, Ivana; Wimmerová, Martina; Sovová, Helena; Wimmer, Zdeněk

2010-01-01

Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn (Hippophae rhamnoides L.) seeds were extracted with supercritical carbon dioxide at pressures ranging from 15–60 MPa and temperatures of 40–80 °C. Oil and β-sitosterol yields were measured in the extraction course and compared with Soxhlet extraction with hexane. The average yield of β-sitosterol was 0.31 mg/g of seeds. The maximum concentration of β-sitosterol in the extract, 0.5% w/w, was achieved at 15 MPa, 40 °C, and a carbon dioxide consumption of 50 g/g of seeds. The extraction rate was maximal at 60 MPa and 40 °C. Both β-sitosterol yield and its concentration in the extract obtained with hexane were lower than with carbon dioxide. PMID:20480045

WRINKLED1 Rescues Feedback Inhibition of Fatty Acid Synthesis in Hydroxylase-Expressing Seeds1[OPEN

PubMed Central

Browse, John

2016-01-01

Previous attempts at engineering Arabidopsis (Arabidopsis thaliana) to produce seed oils containing hydroxy fatty acids (HFA) have resulted in low yields of HFA compared with the native castor (Ricinus communis) plant and caused undesirable effects, including reduced total oil content. Recent studies have led to an understanding of problems involved in the accumulation of HFA in oils of transgenic plants, which include metabolic bottlenecks and a decrease in the rate of fatty acid synthesis. Focusing on engineering the triacylglycerol assembly mechanisms led to modest increases in the HFA content of seed oil, but much room for improvement still remains. We hypothesized that engineering fatty acid synthesis in the plastids to increase flux would facilitate enhanced total incorporation of fatty acids, including HFA, into seed oil. The transcription factor WRINKLED1 (WRI1) positively regulates the expression of genes involved in fatty acid synthesis and controls seed oil levels. We overexpressed Arabidopsis WRI1 in seeds of a transgenic line expressing the castor fatty acid hydroxylase. The proportion of HFA in the oil, the total HFA per seed, and the total oil content of seeds increased to an average of 20.9%, 1.26 µg, and 32.2%, respectively, across five independent lines, compared with 17.6%, 0.83 µg, and 27.9%, respectively, for isogenic segregants. WRI1 and WRI1-regulated genes involved in fatty acid synthesis were up-regulated, providing for a corresponding increase in the rate of fatty acid synthesis. PMID:27208047

Extraction of citral oil from lemongrass (Cymbopogon Citratus) by steam-water distillation technique

NASA Astrophysics Data System (ADS)

Alam, P. N.; Husin, H.; Asnawi, T. M.; Adisalamun

2018-04-01

In Indonesia, production of citral oil from lemon grass (Cymbopogon Cytratus) is done by a traditional technique whereby a low yield results. To improve the yield, an appropriate extraction technology is required. In this research, a steam-water distillation technique was applied to extract the essential oil from the lemongrass. The effects of sample particle size and bed volume on yield and quality of citral oil produced were investigated. The drying and refining time of 2 hours were used as fixed variables. This research results that minimum citral oil yield of 0.53% was obtained on sample particle size of 3 cm and bed volume of 80%, whereas the maximum yield of 1.95% on sample particle size of 15 cm and bed volume of 40%. The lowest specific gravity of 0.80 and the highest specific gravity of 0.905 were obtained on sample particle size of 8 cm with bed volume of 80% and particle size of 12 cm with bed volume of 70%, respectively. The lowest refractive index of 1.480 and the highest refractive index of 1.495 were obtained on sample particle size of 8 cm with bed volume of 70% and sample particle size of 15 cm with bed volume of 40%, respectively. The solubility of the produced citral oil in alcohol was 70% in ratio of 1:1, and the citral oil concentration obtained was around 79%.

Effect of different liming levels on the biomass production and essential oil extraction yield of Cunila galioides Benth.

PubMed

Mossi, A J; Pauletti, G F; Rota, L; Echeverrigaray, S; Barros, I B I; Oliveira, J V; Paroul, N; Cansian, R L

2012-11-01

Poejo is an aromatic and medicinal plant native to highland areas of south Brazil, in acid soils with high Al3+ concentration. The main objective of the present work was to evaluate the effect of liming on the extraction yield of essential oil of three chemotypes of poejo (Cunila galioides Benth). For this purpose, the experiments were performed in a greenhouse, using 8-litre pots. The treatments were four dosages of limestone (0, 3.15, 12.5, and 25 g.L(-1)) and a completely random experimental design was used, with four replications and three chemotypes, set up in a 3 × 4 factorial arrangement. The parameters evaluated were dry weight of aerial parts, essential oil content and chemical composition of essential oil. Results showed that liming affects the biomass production, essential oil yield and chemical composition, with cross interaction verified between chemotype and limestone dosage. For the higher dosage lower biomass production, lower yield of essential oil as well as the lowest content of citral (citral chemotype) and limonene (menthene chemotype) was observed. In the ocimene chemotype, no liming influence was observed on the essential oil yield and on the content of major compounds. The dosage of 3.15 g.L(-1) can be considered the best limestone dosage for the production of poejo for the experimental conditions evaluated.

Oil extraction from Scenedesmus obliquus using a continuous microwave system--design, optimization, and quality characterization.

PubMed

Balasubramanian, Sundar; Allen, James D; Kanitkar, Akanksha; Boldor, Dorin

2011-02-01

A 1.2 kW, 2450 MHz resonant continuous microwave processing system was designed and optimized for oil extraction from green algae (Scenedesmus obliquus). Algae-water suspension (1:1 w/w) was heated to 80 and 95°C, and subjected to extraction for up to 30 min. Maximum oil yield was achieved at 95°C and 30 min. The microwave system extracted 76-77% of total recoverable oil at 20-30 min and 95°C, compared to only 43-47% for water bath control. Extraction time and temperature had significant influence (p<0.0001) on extraction yield. Oil analysis indicated that microwaves extracted oil containing higher percentages of unsaturated and essential fatty acids (indicating higher quality). This study validates for the first time the efficiency of a continuous microwave system for extraction of lipids from algae. Higher oil yields, faster extraction rates and superior oil quality demonstrate this system's feasibility for oil extraction from a variety of feedstock. Copyright © 2010 Elsevier Ltd. All rights reserved.

Parameters influencing the yield and composition of the essential oil from Cretan Vitex agnus-castus fruits.

PubMed

Sørensen, J M; Katsiotis, S T

2000-04-01

Mature and immature fruits of a Cretan Vitex agnus-castus L. population were chosen to investigate different parameters such as comminution, maturity, distillation period and extraction method influencing the essential oil yield and composition. The effect of the comminution and the maturity of the plant material showed highly significant differences in yield and composition of the essential oils obtained, as well as the distillation duration from one to five hours and the method applied (hydrodistillation and simultaneous distillation extraction). The variation of 36 essential oil components due to the parameters applied was studied. The results showed that many different essential oil qualities can be obtained from the same plant material according to the parameters employed in its extraction. Entire fruits hydrodistilled for one hour yielded an oil much richer in monoterpene hydrocarbons and oxygenated compounds whereas the best combination to obtain an oil rich in less volatile compounds is by SDE of comminuted fruits for five hours. For mature fruits the main components varied as follows due to the parameters studied: sabinene 16.4-44.1%, 1,8-cineole 8.4-15.2%, beta-caryophyllene 2.1-5.0%, and trans-beta-farnesene 5.0-11.7%.

[Solidification of volatile oil with graphene oxide].

PubMed

Yan, Hong-Mei; Jia, Xiao-Bin; Zhang, Zhen-Hai; Sun, E; Xu, Yi-Hao

2015-02-01

To evaluate the properties of solidifying volatile oil with graphene oxide, clove oil and zedoary turmeric oil were solidified by graphene oxide. The amount of graphene oxide was optimized with the eugenol yield and curcumol yield as criteria. Curing powder was characterized by differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). The effects of graphene oxide on dissolution in vitro and thermal stability of active components were studied. The optimum solidification ratio of graphene oxide to volatile oil was 1:1. Dissolution rate of active components had rare influence while their thermal stability improved after volatile oil was solidified. Solidifying herbal volatile oil with graphene oxide deserves further study.

Antioxidants from slow pyrolysis bio-oil of birch wood: Application for biodiesel and biobased lubricants

USDA-ARS?s Scientific Manuscript database

Birch wood was slowly pyrolyzed to produce bio-oil and biochar. Slow pyrolysis conditions including reaction temperature, residence time, and particle size of the feed were optimized to maximize bio-oil yield. Particle size had an insignificant effect, whereas yields of up to 56% were achieved using...

Production of biofuel from waste cooking palm oil using nanocrystalline zeolite as catalyst: process optimization studies.

PubMed

Taufiqurrahmi, Niken; Mohamed, Abdul Rahman; Bhatia, Subhash

2011-11-01

The catalytic cracking of waste cooking palm oil to biofuel was studied over different types of nano-crystalline zeolite catalysts in a fixed bed reactor. The effect of reaction temperature (400-500 °C), catalyst-to-oil ratio (6-14) and catalyst pore size of different nanocrystalline zeolites (0.54-0.80 nm) were studied over the conversion of waste cooking palm oil, yields of Organic Liquid Product (OLP) and gasoline fraction in the OLP following central composite design (CCD). The response surface methodology was used to determine the optimum value of the operating variables for maximum conversion as well as maximum yield of OLP and gasoline fraction, respectively. The optimum reaction temperature of 458 °C with oil/catalyst ratio=6 over the nanocrystalline zeolite Y with pore size of 0.67 nm gave 86.4 wt% oil conversion, 46.5 wt% OLP yield and 33.5 wt% gasoline fraction yield, respectively. The experimental results were in agreement with the simulated values within an experimental error of less than 5%. Copyright © 2011 Elsevier Ltd. All rights reserved.

Ex-situ catalytic co-pyrolysis of lignin and polypropylene to upgrade bio-oil quality by microwave heating.

PubMed

Duan, Dengle; Wang, Yunpu; Dai, Leilei; Ruan, Roger; Zhao, Yunfeng; Fan, Liangliang; Tayier, Maimaitiaili; Liu, Yuhuan

2017-10-01

Microwave-assisted fast co-pyrolysis of lignin and polypropylene for bio-oil production was conducted using the ex-situ catalysis technology. Effects of catalytic temperature, feedstock/catalyst ratio, and lignin/polypropylene ratio on product distribution and chemical components of bio-oil were investigated. The catalytic temperature of 250°C was the most conducive to bio-oil production in terms of the yield. The bio-oil yield decreased with the addition of catalyst during ex-situ catalytic co-pyrolysis. When the feedstock/catalyst ratio was 2:1, the minimum char and coke values were 21.22% and 1.54%, respectively. The proportion of cycloalkanes decreased and the aromatics increased with the increasing catalyst loading. A positive synergistic effect was observed between lignin and polypropylene. The char yield dramatically deceased and the bio-oil yield improved during co-pyrolysis compared with those during lignin pyrolysis alone. The proportion of oxygenates dramatically and the minimum value of 6.74% was obtained when the lignin/polypropylene ratio was 1:1. Copyright © 2017. Published by Elsevier Ltd.

Fast Pyrolysis Behavior of Banagrass as a Function of Temperature and Volatiles Residence Time in a Fluidized Bed Reactor

DOE PAGES

Morgan, Trevor James; Turn, Scott Q.; George, Anthe

2015-08-26

A reactor was designed and commissioned to study the fast pyrolysis behavior of banagrass as a function of temperature and volatiles residence time. Four temperatures between 400 and 600°C were examined as well as four residence times between ~1.0 and 10 seconds. Pyrolysis product distributions of bio-oil, char and permanent gases were determined at each reaction condition. The elemental composition of the bio-oils and chars was also assessed. The greatest bio-oil yield was recorded when working at 450°C with a volatiles residence time of 1.4 s, ~37 wt% relative to the dry ash free feedstock (excluding pyrolysis water). The amountsmore » of char (organic fraction) and permanent gases under these conditions are ~4 wt% and 8 wt% respectively. The bio-oil yield stated above is for 'dry' bio-oil after rotary evaporation to remove solvent, which results in volatiles and pyrolysis water being removed from the bio-oil. The material removed during drying accounts for the remainder of the pyrolysis products. The 'dry' bio-oil produced under these conditions contains ~56 wt% carbon which is ~40 wt% of the carbon present in the feedstock. The oxygen content of the 450°C, 1.4 s 'dry' bio-oil is ~38 wt%, which accounts for ~33 wt% of the oxygen in the feedstock. At higher temperature or longer residence time less bio-oil and char is recovered and more gas and light volatiles are produced. Increasing the temperature has a more significant effect on product yields and composition than increasing the volatiles residence time. At 600°C and a volatiles residence time of 1.2 seconds the bio-oil yield is ~21 wt% of the daf feedstock, with a carbon content of 64 wt% of the bio-oil. The bio-oil yield from banagrass is significantly lower than from woody biomass or grasses such as switchgrass or miscanthus, but is similar to barley straw. In conclusion, the reason for the low bio-oil yield from banagrass is thought to be related to its high ash content (8.5 wt% dry basis) and high concentration of alkali and alkali earth metals (totaling ~2.8 wt% relative to the dry feedstock) which are catalytic and increase cracking reactions during pyrolysis.« less

Fast Pyrolysis Behavior of Banagrass as a Function of Temperature and Volatiles Residence Time in a Fluidized Bed Reactor

PubMed Central

Morgan, Trevor James; Turn, Scott Q.; George, Anthe

2015-01-01

A reactor was designed and commissioned to study the fast pyrolysis behavior of banagrass as a function of temperature and volatiles residence time. Four temperatures between 400 and 600°C were examined as well as four residence times between ~1.0 and 10 seconds. Pyrolysis product distributions of bio-oil, char and permanent gases were determined at each reaction condition. The elemental composition of the bio-oils and chars was also assessed. The greatest bio-oil yield was recorded when working at 450°C with a volatiles residence time of 1.4 s, ~37 wt% relative to the dry ash free feedstock (excluding pyrolysis water). The amounts of char (organic fraction) and permanent gases under these conditions are ~4 wt% and 8 wt% respectively. The bio-oil yield stated above is for 'dry' bio-oil after rotary evaporation to remove solvent, which results in volatiles and pyrolysis water being removed from the bio-oil. The material removed during drying accounts for the remainder of the pyrolysis products. The 'dry' bio-oil produced under these conditions contains ~56 wt% carbon which is ~40 wt% of the carbon present in the feedstock. The oxygen content of the 450°C, 1.4 s 'dry' bio-oil is ~38 wt%, which accounts for ~33 wt% of the oxygen in the feedstock. At higher temperature or longer residence time less bio-oil and char is recovered and more gas and light volatiles are produced. Increasing the temperature has a more significant effect on product yields and composition than increasing the volatiles residence time. At 600°C and a volatiles residence time of 1.2 seconds the bio-oil yield is ~21 wt% of the daf feedstock, with a carbon content of 64 wt% of the bio-oil. The bio-oil yield from banagrass is significantly lower than from woody biomass or grasses such as switchgrass or miscanthus, but is similar to barley straw. The reason for the low bio-oil yield from banagrass is thought to be related to its high ash content (8.5 wt% dry basis) and high concentration of alkali and alkali earth metals (totaling ~2.8 wt% relative to the dry feedstock) which are catalytic and increase cracking reactions during pyrolysis. PMID:26308860

Fast Pyrolysis Behavior of Banagrass as a Function of Temperature and Volatiles Residence Time in a Fluidized Bed Reactor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Morgan, Trevor James; Turn, Scott Q.; George, Anthe

A reactor was designed and commissioned to study the fast pyrolysis behavior of banagrass as a function of temperature and volatiles residence time. Four temperatures between 400 and 600°C were examined as well as four residence times between ~1.0 and 10 seconds. Pyrolysis product distributions of bio-oil, char and permanent gases were determined at each reaction condition. The elemental composition of the bio-oils and chars was also assessed. The greatest bio-oil yield was recorded when working at 450°C with a volatiles residence time of 1.4 s, ~37 wt% relative to the dry ash free feedstock (excluding pyrolysis water). The amountsmore » of char (organic fraction) and permanent gases under these conditions are ~4 wt% and 8 wt% respectively. The bio-oil yield stated above is for 'dry' bio-oil after rotary evaporation to remove solvent, which results in volatiles and pyrolysis water being removed from the bio-oil. The material removed during drying accounts for the remainder of the pyrolysis products. The 'dry' bio-oil produced under these conditions contains ~56 wt% carbon which is ~40 wt% of the carbon present in the feedstock. The oxygen content of the 450°C, 1.4 s 'dry' bio-oil is ~38 wt%, which accounts for ~33 wt% of the oxygen in the feedstock. At higher temperature or longer residence time less bio-oil and char is recovered and more gas and light volatiles are produced. Increasing the temperature has a more significant effect on product yields and composition than increasing the volatiles residence time. At 600°C and a volatiles residence time of 1.2 seconds the bio-oil yield is ~21 wt% of the daf feedstock, with a carbon content of 64 wt% of the bio-oil. The bio-oil yield from banagrass is significantly lower than from woody biomass or grasses such as switchgrass or miscanthus, but is similar to barley straw. In conclusion, the reason for the low bio-oil yield from banagrass is thought to be related to its high ash content (8.5 wt% dry basis) and high concentration of alkali and alkali earth metals (totaling ~2.8 wt% relative to the dry feedstock) which are catalytic and increase cracking reactions during pyrolysis.« less

Potential resource materials from Ohio plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Carr, M.E.; Roth, W.B.; Bagby, M.O.

Previously, the Northern Regional Research Center (NRRC) has studied chemical and botanical features of about 800 plant species in a program to identify potential renewable sources of industrial raw materials. In this program, another 64 species from northwestern and southwestern Ohio were studied for the present report. Aboveground samples were quantitatively analyzed for moisture, ash, crude protein, oil, polyphenol, and hydrocarbon. Plant oils were examined for classes of constituents. Oils were saponified and analyzed for yields of organic acids and unsaponifiable matter. Hydrocarbons were examined for the presence of rubber, gutta, and waxes. Rubber and gutta were analyzed for weight-averagemore » molecular weight and molecular weight distribution. Data are presented for 89 species of the 64 that gave the higher chemical yields. Rhus typhina gave outstanding yields of oil (6.5%) and polyphenol (30.8%) (moisture- plus ash-free basis). Liatris aspera yielded substantial amounts of oil (4.7%) and polyphenol (22.7%), whereas Cornus racemosa yielded a substantial amount of oil (4.4%) but a more typical yield of polyphenol (11.0%). Nuphar advena and Epilobium angustifolium had considerable amounts of polyphenol (16.2, 16.3%) but little oil. Nuphar advena contained the most crude protein (24.8%). Noteworthy amounts of hydrocarbon were extracted from Calamagrostis canadensis (1.1%), Aster umbellatus (0.8%), and Solidago riddellii (0.7%). Polyisoprenes in the hydrocarbon fractions of the latter 3 species were identified as gutta for C. canadensis and rubber for A. umbellatus and S. riddellii. Botanical features of the 8 species are briefly discussed.« less

Essential oil composition of sweet basil (Ocimum basilicum L.) in symbiotic relationship with Piriformospora indica and paclobutrazol application under salt stress.

PubMed

Keramati, Sara; Pirdashti, Hemmatollah; Babaeizad, Valliollah; Dehestani, Ali

2016-12-01

Essential oil content and oil composition of paclobutrazol treated sweet basil (Ocimum basilicum L.) plant inoculated with Piriformospora indica under salt stress were investigated by GC-MS. The results show a slight increase in essential oil content when basil plants subjected to moderate salinity stress (3 dS m -1 of NaCl). It decreased signifiicantly with increasing salinity level to 9 dS m -1 . The findings revealed that leaf area, above ground and leaf dry weights, essential oil content and yield were significantly affected by P. indica inoculation, however paclobutrazol application significantly influenced essential oil yield but not content. Fungal symbiosis as well as paclobutrazol application ameliorated the negative effects of salinity on dry matter and essential oil yield. The main constituents found in the volatile oil of O. basilicum in control treatment were Geranial (26.03%), Neral (24.88%) and Estragole (24.78%). The compounds concentrations showed some differences in P. indica and paclobutrazol treatments. The results demonstrate that micorrhiza-like fungi concomitantly increase essential oil production and biomass in sweet basil, a medicinal herb rich in commercially valuable essential oils.

Identification of Rubisco rbcL and rbcS in Camellia oleifera and their potential as molecular markers for selection of high tea oil cultivars

PubMed Central

Chen, Yongzhong; Wang, Baoming; Chen, Jianjun; Wang, Xiangnan; Wang, Rui; Peng, Shaofeng; Chen, Longsheng; Ma, Li; Luo, Jian

2015-01-01

Tea oil derived from seeds of Camellia oleifera Abel. is high-quality edible oil in China. This study isolated full-length cDNAs of Rubisco subunits rbcL and rbcS from C. oleifera. The rbcL has 1,522 bp with a 1,425 bp coding region, encoding 475 amino acids; and the rbcS has 615 bp containing a 528 bp coding region, encoding 176 amino acids. The expression level of the two genes, designated as Co-rbcL and Co-rbcS, was determined in three C. oleifera cultivars: Hengchong 89, Xianglin 1, and Xianglin 14 whose annual oil yields were 546.9, 591.4, and 657.7 kg ha-1, respectively. The Co-rbcL expression in ‘Xianglin 14’ was significantly higher than ‘Xianglin 1’, and ‘Xianglin 1’ was greater than ‘Hengchong 89’. The expression levels of Co-rbcS in ‘Xianglin 1’ and ‘Xianglin 14’ were similar but were significantly greater than in ‘Hengchong 89’. The net photosynthetic rate of ‘Xianglin 14’ was significantly higher than ‘Xianglin 1’, and ‘Xianglin 1’ was higher than ‘Hengchong 89’. Pearson’s correlation analysis showed that seed yields and oil yields were highly correlated with the expression level of Co-rbcL at P < 0.001 level; and the expression of Co-rbcS was correlated with oil yield at P < 0.01 level. Net photosynthetic rate was also correlated with oil yields and seed yields at P < 0.001 and P < 0.01 levels, respectively. Our results suggest that Co-rbcS and Co-rbcL in particular could potentially be molecular markers for early selection of high oil yield cultivars. In combination with the measurement of net photosynthetic rates, the early identification of potential high oil production cultivars would significantly shorten plant breeding time and increase breeding efficiency. PMID:25873921

Utilization of oil extracted from spent coffee grounds for sustainable production of polyhydroxyalkanoates.

PubMed

Obruca, Stanislav; Petrik, Sinisa; Benesova, Pavla; Svoboda, Zdenek; Eremka, Libor; Marova, Ivana

2014-07-01

Spent coffee grounds (SCG), an important waste product of the coffee industry, contain approximately 15 wt% of coffee oil. The aim of this work was to investigate the utilization of oil extracted from SCG as a substrate for the production of poly(3-hydroxybutyrate) (PHB) by Cupriavidus necator H16. When compared to other waste/inexpensive oils, the utilization of coffee oil resulted in the highest biomass as well as PHB yields. Since the correlation of PHB yields and the acid value of oil indicated a positive effect of the presence of free fatty acids in oil on PHB production (correlation coefficient R (2) = 0.9058), superior properties of coffee oil can be probably attributed to the high content of free fatty acids which can be simply utilized by the bacteria culture. Employing the fed-batch mode of cultivation, the PHB yields, the PHB content in biomass, the volumetric productivity, and the Y P/S yield coefficient reached 49.4 g/l, 89.1 wt%, 1.33 g/(l h), and 0.82 g per g of oil, respectively. SCG are annually produced worldwide in extensive amounts and are disposed as solid waste. Hence, the utilization of coffee oil extracted from SCG is likely to improve significantly the economic aspects of PHB production. Moreover, since oil extraction decreased the calorific value of SCG by only about 9 % (from 19.61 to 17.86 MJ/kg), residual SCG after oil extraction can be used as fuel to at least partially cover heat and energy demands of fermentation, which should even improve the economic feasibility of the process.

Effect of the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum on the essential oil yield related characters and nutrient acquisition in the crops of different cultivars of menthol mint (Mentha arvensis) under field conditions.

PubMed

Gupta, M L; Prasad, Arun; Ram, Muni; Kumar, Sushil

2002-01-01

The effects of inoculation with vesicular-arbuscular mycorrhizal (VAM) fungus Glomusfasciculatum on the root colonization, growth, essential oil yield and nutrient acquisition of three cultivars of menthol mint (Mentha arvensis); Kalka, Shivalik and Gomti, were studied under field conditions. The VAM inoculation significantly increased the root colonization, plant height, fresh herbage and dry matter yield. oil content and oil yield as compared to non-inoculated cultivars. The effect of VAM inoculation on the root colonization, growth and yield of mint was more pronounced with the cv Shivalik than the cvs Kalka and Gomati, indicating Shivalik as a highly mycorrhizal dependent genotype. VAM inoculation significantly increased the uptake of N, P and K by shoot tissues of mint, but most markedly increased the uptake of P. The VAM-inoculated mint plants depleted the available N, P and K in the rhizosphere soil as compared to non-inoculated control plants, however the extent of nutrient depletion was greater for P than N and K. We conclude that the VAM inoculation could significantly increase the root colonization, growth, essential oil yield and nutrient acquisition of mint for obtaining economic production under field conditions.

Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species

PubMed Central

2013-01-01

Background Jatropha curcas L. is an oil seed producing non-leguminous tropical shrub that has good potential to be a fuel plant that can be cultivated on marginal land. Due to the low nutrient content of the targeted plantation area, the requirement for fertilizer is expected to be higher than other plants. This factor severely affects the commercial viability of J. curcas. Results We explored the feasibility to use endophytic nitrogen-fixing bacteria that are native to J. curcas to improve plant growth, biomass and seed productivity. We demonstrated that a novel N-fixing endophyte, Enterobacter sp. R4-368, was able to colonize in root and stem tissues and significantly promoted early plant growth and seed productivity of J. curcas in sterilized and non-sterilized soil. Inoculation of young seedling led to an approximately 57.2% increase in seedling vigour over a six week period. At 90 days after planting, inoculated plants showed an average increase of 25.3%, 77.7%, 27.5%, 45.8% in plant height, leaf number, chlorophyll content and stem volume, respectively. Notably, inoculation of the strain led to a 49.0% increase in the average seed number per plant and 20% increase in the average single seed weight when plants were maintained for 1.5 years in non-sterilized soil in pots in the open air. Enterobacter sp. R4-368 cells were able to colonize root tissues and moved systemically to stem tissues. However, no bacteria were found in leaves. Promotion of plant growth and leaf nitrogen content by the strain was partially lost in nifH, nifD, nifK knockout mutants, suggesting the presence of other growth promoting factors that are associated with this bacterium strain. Conclusion Our results showed that Enterobacter sp. R4-368 significantly promoted growth and seed yield of J. curcas. The application of the strains is likely to significantly improve the commercial viability of J. curcas due to the reduced fertilizer cost and improved oil yield. PMID:24083555

Optimization study of Chromalaena odorata essential oil extracted using solventless extraction technique

NASA Astrophysics Data System (ADS)

Nasshorudin, Dalila; Ahmad, Muhammad Syarhabil; Mamat, Awang Soh; Rosli, Suraya

2015-05-01

Solventless extraction process of Chromalaena odorata using reduced pressure and temperature has been investigated. The percentage yield of essential oil produce was calculated for every experiment with different experimental condition. The effect of different parameters, such as temperature and extraction time on the yield was investigated using the Response Surface Methodology (RSM) through Central Composite Design (CCD). The temperature and extraction time were found to have significant effect on the yield of extract. A final essential oil yield was 0.095% could be extracted under the following optimized conditions; a temperature of 80 °C and a time of 8 hours.

Chemical quality and oxidative stability of extra virgin olive oils from San Juan province (Argentina).

PubMed

Ceci, Liliana N; Mattar, Susana B; Carelli, Amalia A

2017-10-01

This study provides information about the chemical quality (quality indices, fatty acid profile, total polyphenols (PPs), tocopherols and pigments) and oxidative stability index (OSI) of virgin olive oils of Arbequina, Changlot Real and Coratina cultivars (San Juan province, Argentina). The influence of the cultivar and the effect of earlier harvest dates on the yields (OY), quality and OSI of the oils were also evaluated. All the oils were classified as extra virgin. The OY (L/100kg) averaged: Arbequina=13.2, Changlot Real=21.3, Coratina=18.3. The oleic acid (O) percentage, oleic to linoleic plus linolenic ratio [O/(L+Ln)], PPs and OSI were highly dependent on cultivar (Arbequina

[Study on absorbing volatile oil with mesoporous carbon].

PubMed

Yan, Hong-mei; Jia, Xiao-bin; Zhang, Zhen-hai; Sun, E; Yang Nan

2014-11-01

Clove oil and turmeric oil were absorbed by mesoporous carbon. The absorption ratio of mesoporous carbon to volatile oil was optimized with the eugenol yield and curcumol yield as criteria Curing powder was characterized by scanning electron microscopy (SEM) and differential scanning calorietry (DSC). The effects of mesoporous carbon on dissolution in vitro and thermal stability of active components were studied. They reached high adsorption rate when the absorption ratio of mesoporous carbon to volatile oil was 1:1. When volatile oil was absorbed, dissolution rate of active components had a little improvement and their thermal stability improved after volatile oil was absorbed by the loss rate decreasing more than 50%. Absorbing herbal volatile oil with mesoporous carbon deserves further studying.

Alternative oil extraction methods from Echium plantagineum L. seeds using advanced techniques and green solvents.

PubMed

Castejón, Natalia; Luna, Pilar; Señoráns, Francisco J

2018-04-01

The edible oil processing industry involves large losses of organic solvent into the atmosphere and long extraction times. In this work, fast and environmentally friendly alternatives for the production of echium oil using green solvents are proposed. Advanced extraction techniques such as Pressurized Liquid Extraction (PLE), Microwave Assisted Extraction (MAE) and Ultrasound Assisted Extraction (UAE) were evaluated to efficiently extract omega-3 rich oil from Echium plantagineum seeds. Extractions were performed with ethyl acetate, ethanol, water and ethanol:water to develop a hexane-free processing method. Optimal PLE conditions with ethanol at 150 °C during 10 min produced a very similar oil yield (31.2%) to Soxhlet using hexane for 8 h (31.3%). UAE optimized method with ethanol at mild conditions (55 °C) produced a high oil yield (29.1%). Consequently, advanced extraction techniques showed good lipid yields and furthermore, the produced echium oil had the same omega-3 fatty acid composition than traditionally extracted oil. Copyright © 2017 Elsevier Ltd. All rights reserved.

The antibacterial and antifungal activity of essential oils extracted from Guatemalan medicinal plants.

PubMed

Miller, Andrew B; Cates, Rex G; Lawrence, Michael; Soria, J Alfonso Fuentes; Espinoza, Luis V; Martinez, Jose Vicente; Arbizú, Dany A

2015-04-01

Essential oils are prevalent in many medicinal plants used for oral hygiene and treatment of diseases. Medicinal plant species were extracted to determine the essential oil content. Those producing sufficient oil were screened for activity against Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. Plant samples were collected, frozen, and essential oils were extracted by steam distillation. Minimum inhibitory concentrations (MIC) were determined using a tube dilution assay for those species yielding sufficient oil. Fifty-nine of the 141 plant species produced sufficient oil for collection and 12 species not previously reported to produce essential oils were identified. Essential oil extracts from 32 species exhibited activity against one or more microbes. Oils from eight species were highly inhibitory to S. mutans, four species were highly inhibitory to C. albicans, and 19 species yielded MIC values less than the reference drugs. RESULTS suggest that 11 species were highly inhibitory to the microbes tested and merit further investigation. Oils from Cinnamomum zeylanicum Blume (Lauraceae), Citrus aurantiifolia (Christm.) Swingle (Rutaceae), Lippia graveolens Kunth (Verbenaceae), and Origanum vulgare L. (Lamiaceae) yielded highly significant or moderate activity against all microbes and have potential as antimicrobial agents. Teas prepared by decoction or infusion are known methods for extracting essential oils. Oils from 11 species were highly active against the microbes tested and merit investigation as to their potential for addressing health-related issues and in oral hygiene.

Analysis of a static undulation on the surface of a thin dielectric liquid layer formed by dielectrophoresis forces

NASA Astrophysics Data System (ADS)

Brown, Carl V.; McHale, Glen; Mottram, Nigel J.

2011-07-01

A layer of insulating liquid of dielectric constant ɛOil and average thickness h- coats a flat surface at y = 0 at which a one-dimensional sinusoidal potential V(x ,0)=VOcos(πx /p) is applied. Dielectrophoresis forces create a static undulation (or "wrinkle") distortion h(x) of period p at the liquid/air interface. Analytical expressions have been derived for the electrostatic energy and the interfacial energy associated with the surface undulation when h(x)=h--(1/2)Acos(2πx /p) yielding a scaling relationship for A as a function of h-, p, VO, ɛOil and the surface tension. The analysis is valid as A/p → 0, and in this limit convergence with numerical simulation of the system is shown.

Use of microbes for paraffin cleanup at Naval Petroleum Reserve No. 3

DOE Office of Scientific and Technical Information (OSTI.GOV)

Giangiacomo, L.; Khatib, A.

1995-12-31

Naval Petroleum Reserve No. 3 (NPR-3), also known as Teapot Dome, is a government-owned oil field in Natrona County, Wyoming. It is an asymmetrical anticline located on the western edge of the Powder River Basin, just south of the Salt Creek Anticline. Production started in 1922, and today the field is a marginally economic stripper field with average production of less than 3 BOPD (0.5 m{sup 3}/D) per well. Total field production is about 1,800 BOPD (286 m{sup 3}/D). The Second Wall Creek Formation was waterflooded from 1979 until June 1992 with poor results due to the extensive natural fracturemore » system in this sandstone unit. Since water injection ceased, reservoir pressure has declined to very low levels. Liquids extraction and reinjection of the gas produced from high-GOR wells along the gas-oil contact continues, but the average gas cap pressure has fallen to approximately 150 psi (1.03 MPa) from an original pressure of 1,120 psi (7.72 MPa). Since the oil is highly paraffinic, wax deposition in the hydraulic fractures and the perforations has become a serious production problem. Microbial treatment was considered as a possible low-cost solution. Four wells were selected in the Second Wall Creek Reservoir with severe paraffin problems and production rates high enough to economically justify the treatment. Problems were experienced with the production of thick oil after approximately three months. This was interpreted to be a result of previously immobile paraffin being cleaned up. A slight decrease in the decline rate was seen in the wells, although some external factors cloud the interpretation. Microbial treatments were discontinued because of marginal economics. Three of the four wells produced additional oil and had a positive incremental cash flow. Oil viscosity tests did indicate that some positive microbial thinning was occurring, and changes to the treatment procedure may potentially yield more economic results in the future.« less

Thermolysis of scrap tire and rubber in sub/super-critical water.

PubMed

Li, Qinghai; Li, Fuxin; Meng, Aihong; Tan, Zhongchao; Zhang, Yanguo

2018-01-01

The rapid growth of waste tires has become a serious environmental issue. Energy and material recovery is regarded as a promising use for waste tires. Thermolysis of scrap tire (ST), natural rubber (NR), and styrene-butadiene rubber (SBR) was carried out in subcritical and supercritical water using a temperature-pressure independent adjustable batch tubular reactor. As a result, oil yields increased as temperature and pressure increased, and they reached maximum values as the state of water was near the critical point. However, further increases in water temperature and pressure reduced the oil yields. The maximum oil yield of 21.21% was obtained at 420 °C and 18 MPa with a reaction time of 40 min. The relative molecular weights of the chemicals in the oil products were in the range of 70-140 g/mole. The oil produced from ST, NR, and SBR contained similar chemical compounds, but the oil yield of SR was between those of NR and SBR. The oil yield from thermolysis of subcritical or supercritical water should be further improved. The main gaseous products, including CH 4 , C 2 H 2 , C 2 H 4 , C 2 H 6 , and C 3 H 8 , increased with reaction time, temperature, and pressure, whereas the solid residues, including carbon black and impurities, decreased. These results provide useful information to develop a sub/super-critical water thermolysis process for energy and material regeneration from waste tires. Copyright © 2017 Elsevier Ltd. All rights reserved.

Correlation between electron spin resonance spectra and oil yield in eastern oil shales

USGS Publications Warehouse

Choudhury, M.; Rheams, K.F.; Harrell, J.W.

1986-01-01

Organic free radical spin concentrations were measured in 60 raw oil shale samples from north Alabama and south Tennessee and compared with Fischer assays and uranium concentrations. No correlation was found between spin concentration and oil yield for the complete set of samples. However, for a 13 sample set taken from a single core hole, a linear correlation was obtained. No correlation between spin concentration and uranium concentration was found. ?? 1986.

Antimicrobial Activity of New Materials Based on Lavender and Basil Essential Oils and Hydroxyapatite.

PubMed

Predoi, Daniela; Iconaru, Simona Liliana; Buton, Nicolas; Badea, Monica Luminita; Marutescu, Luminita

2018-04-30

This study presents, for the first-time, the results of a study on the hydrodynamic diameter of essential oils (EOs) of basil and lavender in water, and solutions of EOs of basil (B) and lavender (L) and hydroxyapatite (HAp). The possible influence of basil and lavender EOs on the size of hydroxyapatite nanoparticles was analyzed by Scanning Electron Microscopy (SEM). We also investigated the in vitro antimicrobial activity of plant EOs and plant EOs hydroxyapatite respectively, against Gram-positive bacteria (methicillin-resistant Staphylococcus aureus 1144 (MRSA 1144) and S. aureus 1426) and Gram-negative bacteria ( Escherichia coli ATCC 25922 and Escherichia coli ESBL 4493). From the autocorrelation function, obtained by Dynamic Light Scattering (DLS) measurements it was observed that basil yielded one peak at an average hydrodynamic diameter of 354.16 nm, while lavender yielded one peak at an average hydrodynamic diameter of 259.76 nm. In the case of HAp nanoparticles coated with basil (HApB) and lavender (HApL) essential oil, the aggregation was minimal. We found that the lavender EO exhibited a very good inhibitory growth activity (MIC values ranging from <0.1% for E. coli reference strain to 0.78% for S. aureus strains). The biological studies indicated that HapL material displayed an enhanced antimicrobial activity, indicating the potential use of HAp as vehicle for low concentrations of lavender EO with antibacterial properties. Flow cytometry analysis (FCM) allowed us to determine some of the potential mechanisms of the antimicrobial activities of EOs, suggesting that lavender EO was active against E. coli by interfering with membrane potential, the membrane depolarization effect being increased by incorporation of the EOs into the microporous structure of HAp. These findings could contribute to the development of new antimicrobial agents that are urgently needed for combating the antibiotic resistance phenomena.

Co-pyrolysis of rice straw and polypropylene using fixed-bed pyrolyzer

NASA Astrophysics Data System (ADS)

Izzatie, N. I.; Basha, M. H.; Uemura, Y.; Mazlan, M. A.; Hashim, M. S. M.; Amin, N. A. M.; Hamid, M. F.

2016-11-01

The present work encompasses the impact of temperature (450, 500, 550, 600 °C) on the properties of pyrolysis oil and on other product yield for the co-pyrolysis of Polypropylene (PP) plastics and rice straw. Co-pyrolysis of PP plastic and rice straw were conducted in a fixed-bed drop type pyrolyzer under an inert condition to attain maximum oil yield. Physically, the pyrolysis oil is dark-brown in colour with free flowing and has a strong acrid smell. Copyrolysis between these typically obtained in maximum pyrolysis oil yields up to 69% by ratio 1:1 at a maximum temperature of 550 °C. From the maximum yield of pyrolysis oil, characterization of pyrolysis product and effect of biomass type of the composition were evaluated. Pyrolysis oil contains a high water content of 66.137 wt.%. Furfural, 2- methylnaphthalene, tetrahydrofuran (THF), toluene and acetaldehyde were the major organic compounds found in pyrolysis oil of rice straw mixed with PP. Bio-char collected from co-pyrolysis of rice straw mixed with PP plastic has high calorific value of 21.190 kJ/g and also carbon content with 59.02 wt.% and could contribute to high heating value. The non-condensable gases consist of hydrogen, carbon monoxide, and methane as the major gas components.

Optimizing oil and xanthorrhizol extraction from Curcuma xanthorrhiza Roxb. rhizome by supercritical carbon dioxide.

PubMed

Salea, Rinaldi; Widjojokusumo, Edward; Veriansyah, Bambang; Tjandrawinata, Raymond R

2014-09-01

Oil and xanthorrhizol extraction from Curcuma xanthorrhiza Roxb. rhizome by supercritical carbon dioxide was optimized using Taguchi method. The factors considered were pressure, temperature, carbon dioxide flowrate and time at levels ranging between 10-25 MPa, 35-60 °C, 10-25 g/min and 60-240 min respectively. The highest oil yield (8.0 %) was achieved at factor combination of 15 MPa, 50 °C, 20 g/min and 180 min whereas the highest xanthorrhizol content (128.3 mg/g oil) in Curcuma xanthorrhiza oil was achieved at a factor combination of 25 MPa, 50 °C, 15 g/min and 60 min. Soxhlet extraction with n-hexane and percolation with ethanol gave oil yield of 5.88 %, 11.73 % and xanthorrhizol content of 42.6 mg/g oil, 75.5 mg/g oil, respectively. The experimental oil yield and xanthorrhizol content at optimum conditions agreed favourably with values predicted by computational process. The xanthorrizol content extracted using supercritical carbon dioxide was higher than extracted using Soxhlet extraction and percolation process.

Recovery of Palm Oil and Valuable Material from Oil Palm Empty Fruit Bunch by Sub-critical Water.

PubMed

Ahmad Kurnin, Nor Azrin; Shah Ismail, Mohd Halim; Yoshida, Hiroyuki; Izhar, Shamsul

2016-01-01

Oil palm empty fruit bunch (EFB) is one of the solid wastes produced in huge volume by palm oil mill. Whilst it still contains valuable oil, approximately 22.6 million tons is generated annually and treated as solid waste. In this work, sub-critical water (sub-cw) was used to extract oil, sugar and tar from spikelet of EFB. The spikelet was treated with sub-cw between 180-280°C and a reaction time of 2 and 5 minutes. The highest yield of oil was 0.075 g-oil/g-dry EFB, obtained at 240°C and reaction time of 5 minutes. Astonishingly, oil that was extracted through this method was 84.5% of that obtained through Soxhlet method using hexane. Yield of oil extracted was strongly affected by the reaction temperature and time. Higher reaction temperature induces the dielectric constant of water towards the non-polar properties of solvent; thus increases the oil extraction capability. Meanwhile, the highest yield of sugar was 0.20 g-sugar/g-dry EFB obtained at 220°C. At this temperature, the ion product of water is high enough to enable maximum sub-critical water hydrolysis reaction. This study showed that oil and other valuable material can be recovered using water at sub-critical condition, and most attractive without the use of harmful organic solvent.

Oil shale potential of the Heath and Tyler formations, Central Montana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cox, W.E.; Cole, G.A.

The units in the middle of the Heath formation below the gypsum beds were found to have the highest oil yields. That interval was generally 25 to 50 ft (7.6 to 15.2 m) thick. The upper portion of the Heath formation yielded as much as 9.8 gal/ton in section 9, and 14.9 gal/ton in section 10. The Tyler formation was determined to have very low oil potential, with the maximum yield being 2.2 gal/ton. The instability of some of the Heath slopes could present problems in the mining of oil shale. Specific stratigraphic horizons in which zones of high andmore » low oil and metal contents occur would be extremely difficult to map in areas where the units have been displaced by landslide movement.« less

Modification of aqueous enzymatic oil extraction to increase the yield of corn oil from dry fractionated corn germ

USDA-ARS?s Scientific Manuscript database

In previous aqueous enzymatic extraction experiments we reported an oil yield of 67 grams from 800 grams of dry fractionated corn germ. In the current experiments, a dispersion of 10% cooked, dry-fractionated germ in water and was treated with amylases and a cellulase complex. A foam fraction was s...

Optimization of Supercritical CO2 Extraction of Fish Oil from Viscera of African Catfish (Clarias gariepinus)

PubMed Central

Sarker, Mohamed Zaidul Islam; Selamat, Jinap; Habib, Abu Sayem Md. Ahsan; Ferdosh, Sahena; Akanda, Mohamed Jahurul Haque; Jaffri, Juliana Mohamed

2012-01-01

Fish oil was extracted from the viscera of African Catfish using supercritical carbon dioxide (SC-CO2). A Central Composite Design of Response Surface methodology (RSM) was employed to optimize the SC-CO2 extraction parameters. The oil yield (Y) as response variable was executed against the four independent variables, namely pressure, temperature, flow rate and soaking time. The oil yield varied with the linear, quadratic and interaction of pressure, temperature, flow rate and soaking time. Optimum points were observed within the variables of temperature from 35 °C to 80 °C, pressure from 10 MPa to 40 MPa, flow rate from 1 mL/min to 3 mL/min and soaking time from 1 h to 4 h. However, the extraction parameters were found to be optimized at temperature 57.5 °C, pressure 40 MPa, flow rate 2.0 mL/min and soaking time 2.5 h. At this optimized condition, the highest oil yields were found to be 67.0% (g oil/100 g sample on dry basis) in the viscera of catfish which was reasonable to the yields of 78.0% extracted using the Soxhlet method. PMID:23109854

Influence of temperature and reaction time on the conversion of polystyrene waste to pyrolysis liquid oil.

PubMed

Miandad, R; Nizami, A S; Rehan, M; Barakat, M A; Khan, M I; Mustafa, A; Ismail, I M I; Murphy, J D

2016-12-01

This paper aims to investigate the effect of temperature and reaction time on the yield and quality of liquid oil produced from a pyrolysis process. Polystyrene (PS) type plastic waste was used as a feedstock in a small pilot scale batch pyrolysis reactor. At 400°C with a reaction time of 75min, the gas yield was 8% by mass, the char yield was 16% by mass, while the liquid oil yield was 76% by mass. Raising the temperature to 450°C increased the gas production to 13% by mass, reduced the char production to 6.2% and increased the liquid oil yield to 80.8% by mass. The optimum temperature and reaction time was found to be 450°C and 75min. The liquid oil at optimum conditions had a dynamic viscosity of 1.77mPas, kinematic viscosity of 1.92cSt, a density of 0.92g/cm 3 , a pour point of -60°C, a freezing point of -64°C, a flash point of 30.2°C and a high heating value (HHV) of 41.6MJ/kg this is similar to conventional diesel. The gas chromatography with mass spectrophotometry (GC-MS) analysis showed that liquid oil contains mainly styrene (48%), toluene (26%) and ethyl-benzene (21%) compounds. Copyright © 2016 Elsevier Ltd. All rights reserved.

Multigene Engineering of Triacylglycerol Metabolism Boosts Seed Oil Content in Arabidopsis1[W][OPEN

PubMed Central

van Erp, Harrie; Kelly, Amélie A.; Menard, Guillaume; Eastmond, Peter J.

2014-01-01

Increasing the yield of oilseed crops is an important objective for biotechnologists. A number of individual genes involved in triacylglycerol metabolism have previously been reported to enhance the oil content of seeds when their expression is altered. However, it has yet to be established whether specific combinations of these genes can be used to achieve an additive effect and whether this leads to enhanced yield. Using Arabidopsis (Arabidopsis thaliana) as an experimental system, we show that seed-specific overexpression of WRINKLED1 (a transcriptional regulator of glycolysis and fatty acid synthesis) and DIACYLGLYCEROL ACYLTRANSFERASE1 (a triacylglycerol biosynthetic enzyme) combined with suppression of the triacylglycerol lipase SUGAR-DEPENDENT1 results in a higher percentage seed oil content and greater seed mass than manipulation of each gene individually. Analysis of total seed yield per plant suggests that, despite a reduction in seed number, the total yield of oil is also increased. PMID:24696520

Ridge sowing of sunflower (Helianthus annuus L.) in a minimum till system improves the productivity, oil quality, and profitability on a sandy loam soil under an arid climate.

PubMed

Sher, Ahmad; Suleman, Muhammad; Qayyum, Abdul; Sattar, Abdul; Wasaya, Allah; Ijaz, Muhammad; Nawaz, Ahmad

2018-04-01

Sunflower (Helianthus annuus L.) is a major oilseed crop grown for its edible oil across the globe including Pakistan. In Pakistan, the production of edible oil is less than the required quantity; the situation is being worsened with the increasing population. Thus, there is dire need to grow those sunflower genotypes which perform better under a given set of agronomic practices. In this 2-year study, we compared four sunflower genotypes, viz., Armoni, Kundi, Sinji, and S-278 for their yield potential, oil contents, fatty acid composition, and profitability under three sowing methods, viz., bed sowing, line sowing, and ridge sowing and two tillage system, viz., plow till and minimum till. Among the sunflower genotypes, the genotype Armoni produced the highest plant height, number of leaves, head diameter, 1000-achene weight, and achene yield; the oil contents and oleic acid were the highest in genotype Sinji. Among the sowing methods, the highest number of leaves per plant, head diameter, number of achenes per head, achene yield, and oil contents were recorded in ridge sowing. Among the tillage systems, the highest head diameter 16. 2 cm, 1000-achene weight (57.2 g), achene yield (1.8 t ha -1 ), oil contents (35.2%), and oleic acid (15.2%) were recorded in minimum till sunflower. The highest net benefits and benefit to cost ratio were recorded in minimum till ridge sown Armoni genotype. In conclusion, the genotype Armoni should be grown on ridges to achieve the highest achene yield, oil contents, and net profitability.

Fast pyrolysis of tropical biomass species and influence of water pretreatment on product distributions

DOE PAGES

Morgan, Trevor James; Turn, Scott Q.; Sun, Ning; ...

2016-03-15

Here, the fast pyrolysis behaviour of pretreated banagrass was examined at four temperatures (between 400 and 600 C) and four residence times (between ~1.2 and 12 s). The pretreatment used water washing/leaching to reduce the inorganic content of the banagrass. Yields of bio-oil, permanent gases and char were determined at each reaction condition and compared to previously published results from untreated banagrass. Comparing the bio-oil yields from the untreated and pretreated banagrass shows that the yields were greater from the pretreated banagrass by 4 to 11 wt% (absolute) at all reaction conditions. The effect of pretreatment (i.e. reducing the amountmore » of ash, and alkali and alkali earth metals) on pyrolysis products is: 1) to increase the dry bio-oil yield, 2) to decrease the amount of undetected material, 3) to produce a slight increase in CO yield or no change, 4) to slightly decrease CO 2 yield or no change, and 5) to produce a more stable bio-oil (less aging). Char yield and total gas yield were unaffected by feedstock pretreatment. Four other tropical biomass species were also pyrolyzed under one condition (450°C and 1.4 s residence time) for comparison to the banagrass results. The samples include two hardwoods: leucaena and eucalyptus, and two grasses: sugarcane bagasse and energy-cane. A sample of pretreated energy-cane was also pyrolyzed. Of the materials tested, the best feedstocks for fast pyrolysis were sugarcane bagasse, pretreated energy cane and eucalyptus based on the yields of 'dry bio-oil', CO and CO 2. On the same basis, the least productive feedstocks are untreated banagrass followed by pretreated banagrass and leucaena.« less

Fast pyrolysis of tropical biomass species and influence of water pretreatment on product distributions

DOE Office of Scientific and Technical Information (OSTI.GOV)

Morgan, Trevor James; Turn, Scott Q.; Sun, Ning

Here, the fast pyrolysis behaviour of pretreated banagrass was examined at four temperatures (between 400 and 600 C) and four residence times (between ~1.2 and 12 s). The pretreatment used water washing/leaching to reduce the inorganic content of the banagrass. Yields of bio-oil, permanent gases and char were determined at each reaction condition and compared to previously published results from untreated banagrass. Comparing the bio-oil yields from the untreated and pretreated banagrass shows that the yields were greater from the pretreated banagrass by 4 to 11 wt% (absolute) at all reaction conditions. The effect of pretreatment (i.e. reducing the amountmore » of ash, and alkali and alkali earth metals) on pyrolysis products is: 1) to increase the dry bio-oil yield, 2) to decrease the amount of undetected material, 3) to produce a slight increase in CO yield or no change, 4) to slightly decrease CO 2 yield or no change, and 5) to produce a more stable bio-oil (less aging). Char yield and total gas yield were unaffected by feedstock pretreatment. Four other tropical biomass species were also pyrolyzed under one condition (450°C and 1.4 s residence time) for comparison to the banagrass results. The samples include two hardwoods: leucaena and eucalyptus, and two grasses: sugarcane bagasse and energy-cane. A sample of pretreated energy-cane was also pyrolyzed. Of the materials tested, the best feedstocks for fast pyrolysis were sugarcane bagasse, pretreated energy cane and eucalyptus based on the yields of 'dry bio-oil', CO and CO 2. On the same basis, the least productive feedstocks are untreated banagrass followed by pretreated banagrass and leucaena.« less

Fast Pyrolysis of Tropical Biomass Species and Influence of Water Pretreatment on Product Distributions

PubMed Central

Morgan, Trevor James; Turn, Scott Q.; Sun, Ning; George, Anthe

2016-01-01

The fast pyrolysis behaviour of pretreated banagrass was examined at four temperatures (between 400 and 600 C) and four residence times (between ~1.2 and 12 s). The pretreatment used water washing/leaching to reduce the inorganic content of the banagrass. Yields of bio-oil, permanent gases and char were determined at each reaction condition and compared to previously published results from untreated banagrass. Comparing the bio-oil yields from the untreated and pretreated banagrass shows that the yields were greater from the pretreated banagrass by 4 to 11 wt% (absolute) at all reaction conditions. The effect of pretreatment (i.e. reducing the amount of ash, and alkali and alkali earth metals) on pyrolysis products is: 1) to increase the dry bio-oil yield, 2) to decrease the amount of undetected material, 3) to produce a slight increase in CO yield or no change, 4) to slightly decrease CO2 yield or no change, and 5) to produce a more stable bio-oil (less aging). Char yield and total gas yield were unaffected by feedstock pretreatment. Four other tropical biomass species were also pyrolyzed under one condition (450°C and 1.4 s residence time) for comparison to the banagrass results. The samples include two hardwoods: leucaena and eucalyptus, and two grasses: sugarcane bagasse and energy-cane. A sample of pretreated energy-cane was also pyrolyzed. Of the materials tested, the best feedstocks for fast pyrolysis were sugarcane bagasse, pretreated energy cane and eucalyptus based on the yields of 'dry bio-oil', CO and CO2. On the same basis, the least productive feedstocks are untreated banagrass followed by pretreated banagrass and leucaena. PMID:26978265

Fast Pyrolysis of Tropical Biomass Species and Influence of Water Pretreatment on Product Distributions.

PubMed

Morgan, Trevor James; Turn, Scott Q; Sun, Ning; George, Anthe

2016-01-01

The fast pyrolysis behaviour of pretreated banagrass was examined at four temperatures (between 400 and 600 C) and four residence times (between ~1.2 and 12 s). The pretreatment used water washing/leaching to reduce the inorganic content of the banagrass. Yields of bio-oil, permanent gases and char were determined at each reaction condition and compared to previously published results from untreated banagrass. Comparing the bio-oil yields from the untreated and pretreated banagrass shows that the yields were greater from the pretreated banagrass by 4 to 11 wt% (absolute) at all reaction conditions. The effect of pretreatment (i.e. reducing the amount of ash, and alkali and alkali earth metals) on pyrolysis products is: 1) to increase the dry bio-oil yield, 2) to decrease the amount of undetected material, 3) to produce a slight increase in CO yield or no change, 4) to slightly decrease CO2 yield or no change, and 5) to produce a more stable bio-oil (less aging). Char yield and total gas yield were unaffected by feedstock pretreatment. Four other tropical biomass species were also pyrolyzed under one condition (450°C and 1.4 s residence time) for comparison to the banagrass results. The samples include two hardwoods: leucaena and eucalyptus, and two grasses: sugarcane bagasse and energy-cane. A sample of pretreated energy-cane was also pyrolyzed. Of the materials tested, the best feedstocks for fast pyrolysis were sugarcane bagasse, pretreated energy cane and eucalyptus based on the yields of 'dry bio-oil', CO and CO2. On the same basis, the least productive feedstocks are untreated banagrass followed by pretreated banagrass and leucaena.

Essential oil composition and antifungal activity of Foeniculum vulgare Mill obtained by different distillation conditions.

PubMed

Mimica-Dukić, N; Kujundzić, S; Soković, M; Couladis, M

2003-04-01

The influence of different hydrodistillation conditions was evaluated from the standpoint of essential oil yield, chemical composition and antifungal activity from seeds of Foeniculum vulgare Mill. Three hydrodistillation conditions were considered. The main constituents of the oils were: (E)-anethole (72.27%-74.18%), fenchone (11.32%-16.35%) and methyl chavicol (3.78%-5.29%). The method of distillation significantly effected the essential oil yield and quantitative composition, although the antifungal activity of the oils against some fungi was only slightly altered. Copyright 2003 John Wiley & Sons, Ltd.

Geochemistry of Israeli oil shales: a review

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shirav, M.; Ginzburg, D.

1983-01-01

The oil shales of Israel are widely distributed throughout the country and have current reserves of about 3500 million tons located in the following deposits: Zin, Oron, Ef'e, Hartuv, and Nabi-Musa. The geochemistry and chemical analysis of these shales are discussed, along with the calorific value, oil yield, and trace elements. The main components influencing the quality of the oil shales are organic matter, carbonate, clay minerals, and apatite. Compositional variations within the organic matter are responsible for changes in the relative calorific value and retorted oil yield while fluidized bed combustion is affected by the inorganic components. (JMT)

Factorial design of essential oil extraction from Fagraea fragrans Roxb. flowers and evaluation of its biological activities for perfumery and cosmetic applications.

PubMed

Yingngam, B; Brantner, A H

2015-06-01

To optimize the extraction yields of essential oil from Fagraea fragrans Roxb. flowers in hydro-distillation using a central composite design (CCD) and to evaluate its biological activities for perfumery and cosmetic applications. Central composite design was applied to study the influences of operational parameters [water to flower weight (X(1)) and distillation time (X(2))] on the yields of essential oil (Y). Chemical compositions of the essential oil extracted from the optimized condition were identified by gas chromatography-mass spectrometry. Antioxidant activities of the essential oil were determined against ABTS(•+) and DPPH(•) radicals, and the cytotoxic effects were assessed on human embryonic kidney (HEK293) cells by the use of the MTT assay. Also, the aromatic properties of the essential oil were evaluated by five healthy trained volunteers. The best conditions to obtain the maximum essential oil yield were 7.5 mL g(-1) (X(1)) and 215 min (X(2)). The experimental yield of the essential oil (0.35 ± 0.02% v/w) was close to the value predicted by a mathematical model (0.35 ± 0.01% v/w). 3-Octadecyne, Z,Z,Z-7,10,13-hexadecatrienal, E-nerolidol, pentadecanal and linalool were the major constituents of the essential oil. The essential oil showed moderate antioxidant capacities with no toxic effects on HEK293 cells at 1-250 μg mL(-1). Also, the essential oil exhibited a very strong aroma and was classified to be top- to middle-notes. The results offer the effectively operational conditions in the extraction of essential oil from F. fragrans using hydro-distillation. The essential oil could be used as a natural fragrance, having antioxidant activity with slight cytotoxicity, for perfumery and cosmetic applications. © 2014 Society of Cosmetic Scientists and the Société Française de Cosmétologie.

Intensification of biodiesel production from soybean oil and waste cooking oil in the presence of heterogeneous catalyst using high speed homogenizer.

PubMed

Joshi, Saurabh; Gogate, Parag R; Moreira, Paulo F; Giudici, Reinaldo

2017-11-01

In the present work, high speed homogenizer has been used for the intensification of biodiesel synthesis from soybean oil and waste cooking oil (WCO) used as a sustainable feedstock. High acid value waste cooking oil (27mg of KOH/g of oil) was first esterified with methanol using sulphuric acid as catalyst in two stages to bring the acid value to desired value of 1.5mg of KOH/g of oil. Transesterification of soybean oil (directly due to lower acid value) and esterified waste cooking oil was performed in the presence of heterogeneous catalyst (CaO) for the production of biodiesel. Various experiments were performed for understanding the effect of operating parameters viz. molar ratio, catalyst loading, reaction temperature and speed of rotation of the homogenizer. For soybean oil, the maximum biodiesel yield as 84% was obtained with catalyst loading of 3wt% and molar ratio of oil to methanol of 1:10 at 50°C with 12,000rpm as the speed of rotation in 30min. Similarly biodiesel yield of 88% was obtained from waste cooking oil under identical operating conditions except for the catalyst loading which was 1wt%. Significant increase in the rate of biodiesel production with yields from soybean oil as 84% (in 30min) and from WCO as 88% (30min) was established due to the use of high speed homogenizer as compared to the conventional stirring method (requiring 2-3h for obtaining similar biodiesel yield). The observed intensification was attributed to the turbulence caused at microscale and generation of fine emulsions due to the cavitational effects. Overall it can be concluded from this study that high speed homogenizer can be used as an alternate cavitating device to efficiently produce biodiesel in the presence of heterogeneous catalysts. Copyright © 2017 Elsevier B.V. All rights reserved.

Microwave-assisted co-pyrolysis of brown coal and corn stover for oil production.

PubMed

Zhang, Yaning; Fan, Liangliang; Liu, Shiyu; Zhou, Nan; Ding, Kuan; Peng, Peng; Anderson, Erik; Addy, Min; Cheng, Yanling; Liu, Yuhuan; Li, Bingxi; Snyder, John; Chen, Paul; Ruan, Roger

2018-07-01

The controversial synergistic effect between brown coal and biomass during co-pyrolysis deserves further investigation. This study detailed the oil production from microwave-assisted co-pyrolysis of brown coal (BC) and corn stover (CS) at different CS/BC ratios (0, 0.33, 0.50, 0.67, and 1) and pyrolysis temperatures (500, 550, and 600 °C). The results showed that a higher CS/BC ratio resulted in higher oil yield, and a higher pyrolysis temperature increased oil yield for brown coal and coal/corn mixtures. Corn stover and brown coal showed different pyrolysis characteristics, and positive synergistic effect on oil yield was observed only at CS/BC ratio of 0.33 and pyrolysis temperature of 600 °C. Oils from brown coal mainly included hydrocarbons and phenols whereas oils from corn stover and coal/corn mixtures were dominated by ketones, phenols, and aldehydes. Positive synergistic effects were observed for ketones, aldehydes, acids, and esters whereas negative synergistic effects for hydrocarbons, phenols and alcohols. Copyright © 2018 Elsevier Ltd. All rights reserved.

Production of bio-oil and biochar from soapstock via microwave-assisted co-catalytic fast pyrolysis.

PubMed

Dai, Leilei; Fan, Liangliang; Liu, Yuhuan; Ruan, Roger; Wang, Yunpu; Zhou, Yue; Zhao, Yunfeng; Yu, Zhenting

2017-02-01

In this study, production of bio-oil and biochar from soapstock via microwave-assisted co-catalytic fast pyrolysis combining the advantages of in-situ and ex-situ catalysis was performed. The effects of catalyst and pyrolysis temperature on product fractional yields and bio-oil chemical compositions were investigated. From the perspective of bio-oil yield, the optimal pyrolysis temperature was 550°C. The use of catalysts reduced the water content, and the addition of bentonite increased the bio-oil yield. Up to 84.16wt.% selectivity of hydrocarbons in the bio-oil was obtained in the co-catalytic process. In addition, the co-catalytic process can reduce the proportion of oxygenates in the bio-oil to 15.84wt.% and eliminate the N-containing compounds completely. The addition of bentonite enhanced the BET surface area of bio-char. In addition, the bio-char removal efficiency of Cd 2+ from soapstock pyrolysis in presence of bentonite was 27.4wt.% higher than without bentonite. Copyright © 2016 Elsevier Ltd. All rights reserved.

Green ultrasound-assisted extraction of carotenoids from pomegranate wastes using vegetable oils.

PubMed

Goula, Athanasia M; Ververi, Maria; Adamopoulou, Anna; Kaderides, Kyriakos

2017-01-01

The objective of this work was to develop a new process for pomegranate peels application in food industries based on ultrasound-assisted extraction of carotenoids using different vegetable oils as solvents. In this way, an oil enriched with antioxidants is produced. Sunflower oil and soy oil were used as alternative solvents and the effects of various parameters on extraction yield were studied. Extraction temperature, solid/oil ratio, amplitude level, and extraction time were the factors investigated with respect to extraction yield. Comparative studies between ultrasound-assisted and conventional solvent extraction were carried out in terms of processing procedure and total carotenoids content. The efficient extraction period for achieving maximum yield of pomegranate peel carotenoids was about 30min. The optimum operating conditions were found to be: extraction temperature, 51.5°C; peels/solvent ratio, 0.10; amplitude level, 58.8%; solvent, sunflower oil. A second-order kinetic model was successfully developed for describing the mechanism of ultrasound extraction under different processing parameters. Copyright © 2016 Elsevier B.V. All rights reserved.

Response of Biomass Development, Essential Oil, and Composition of Plectranthus amboinicus (Lour.) Spreng. to Irrigation Frequency and Harvest Time.

PubMed

Sabra, Ali S; Astatkie, Tessema; Alataway, Abed; Mahmoud, Abeer A; Gendy, Ahmed S H; Said-Al Ahl, Hussein A H; Tkachenko, Kirill G

2018-03-01

A greenhouse experiment was conducted to study the effects of four irrigation intervals (4, 8, 12, and 16 days) and six harvests (2, 4, 6, 8, 10, and 12 months after transplanting) on biomass, essential oil content, and composition of Plectranthus amboinicus (Lour.) Spreng. Fresh weight and essential oil yield decreased with increasing irrigation interval; whereas, essential oil content was stimulated by water stress and increased as the irrigation interval increased. Fresh weight of Plectranthus amboinicus irrigated every 4 days peaked when harvested at 6 months, but essential oil content peaked when irrigated every 16 days and harvested at 2 months after transplantation. On the other hand, essential oil yield peaked when irrigated every 8 days and harvested at 6 months. Thymol, p-cymene, γ-terpinene, and β-caryophyllene were the major compounds, and they peaked at different irrigation intervals and harvest times. This study showed biomass, essential oil content, and yield as well as the major and minor constituents of Plectranthus amboinicus are influenced by irrigation interval and the timing of harvest. © 2018 Wiley-VHCA AG, Zurich, Switzerland.

Two-step fast microwave-assisted pyrolysis of biomass for bio-oil production using microwave absorbent and HZSM-5 catalyst.

PubMed

Zhang, Bo; Zhong, Zhaoping; Xie, Qinglong; Liu, Shiyu; Ruan, Roger

2016-07-01

A novel technology of two-step fast microwave-assisted pyrolysis (fMAP) of corn stover for bio-oil production was investigated in the presence of microwave absorbent (SiC) and HZSM-5 catalyst. Effects of fMAP temperature and catalyst-to-biomass ratio on bio-oil yield and chemical components were examined. The results showed that this technology, employing microwave, microwave absorbent and HZSM-5 catalyst, was effective and promising for biomass fast pyrolysis. The fMAP temperature of 500°C was considered the optimum condition for maximum yield and best quality of bio-oil. Besides, the bio-oil yield decreased linearly and the chemical components in bio-oil were improved sequentially with the increase of catalyst-to-biomass ratio from 1:100 to 1:20. The elemental compositions of bio-char were also determined. Additionally, compared to one-step fMAP process, two-step fMAP could promote the bio-oil quality with a smaller catalyst-to-biomass ratio. Copyright © 2016. Published by Elsevier B.V.

Thermal and catalytic slow pyrolysis of Calophyllum inophyllum fruit shell.

PubMed

Alagu, R M; Sundaram, E Ganapathy; Natarajan, E

2015-10-01

Pyrolysis of Calophyllum inophyllum shell was performed in a fixed bed pyrolyser to produce pyrolytic oil. Both thermal (without catalysts) and catalytic pyrolysis process were conducted to investigate the effect of catalysts on pyrolysis yield and pyrolysis oil characteristics. The yield of pyrolytic oil through thermal pyrolysis was maximum (41% wt) at 425 °C for particle size of 1.18 mm and heating rate of 40 °C/min. In catalytic pyrolysis the pyrolytic oil yield was maximum (45% wt) with both zeolite and kaolin catalysts followed by Al2O3 catalyst (44% wt). The functional groups and chemical components present in the pyrolytic oil are identified by Fourier Transform Infrared Spectroscopy (FT-IR) and Gas Chromatography-Mass Spectrometry (GC-MS) techniques. This study found that C. inophyllum shell is a potential new green energy source and that the catalytic pyrolysis process using zeolite catalyst improves the calorific value and acidity of the pyrolytic oil. Copyright © 2015 Elsevier Ltd. All rights reserved.

Microwave pyrolysis of oily sludge with activated carbon.

PubMed

Chen, Yi-Rong

2016-12-01

The aim of this study is to explore catalytic microwave pyrolysis of crude oil storage tank sludge for fuels using granular activated carbon (GAC) as a catalyst. The effect of GAC loading on the yield of pyrolysis products was also investigated. Heating rate of oily sludge and yield of microwave pyrolysis products such as oil and fuel gas was found to depend on the ratio of GAC to oily sludge. The optimal GAC loading was found to be 10%, while much smaller and larger feed sizes adversely influenced production. During oily sludge pyrolysis, a maximum oil yield of 77.5% was achieved. Pyrolytic oils with high concentrations of diesel oil and gasoline (about 70 wt% in the pyrolytic oil) were obtained. The leaching of heavy metals, such as Cr, As and Pb, was also suppressed in the solid residue after pyrolysis. This technique provides advantages such as harmless treatment of oily sludge and substantial reduction in the consumption of energy, time and cost.

Factors affecting the yield of bio-oil from the pyrolysis of coconut shell.

PubMed

Gao, Yun; Yang, Yi; Qin, Zhanbin; Sun, Yi

2016-01-01

Coconut is a high-quality agricultural product of the Asia-Pacific region. In this paper, coconut shell which mainly composed of cellulose, hemicellulose, lignin was used as a raw material for coconut shell oil from coconut shell pyrolysis. The influence of the pyrolysis temperature, heating rate and particle size on coconut oil yield was investigated, and the effect of heating rate on coconut oil components was discussed. Experimental results show that the maximum oil yield of 75.74 wt% (including water) were obtained under the conditions that the final pyrolysis temperature 575 °C, heating rate 20 °C/min, coconut shell diameter about 5 mm. Thermal gravimetric analysis was used and it can be seen that coconut shell pyrolysis process can be divided into three stages: water loss, pyrolysis and pyrocondensation. The main components of coconut-shell oil are water (about 50 wt%), aromatic, phenolic, acid, ketone and ether containing compounds.

Catalytic pyrolysis of olive mill wastewater sludge

NASA Astrophysics Data System (ADS)

Abdellaoui, Hamza

From 2008 to 2013, an average of 2,821.4 kilotons/year of olive oil were produced around the world. The waste product of the olive mill industry consists of solid residue (pomace) and wastewater (OMW). Annually, around 30 million m3 of OMW are produced in the Mediterranean area, 700,000 m3 year?1 in Tunisia alone. OMW is an aqueous effluent characterized by an offensive smell and high organic matter content, including high molecular weight phenolic compounds and long-chain fatty acids. These compounds are highly toxic to micro-organisms and plants, which makes the OMW a serious threat to the environment if not managed properly. The OMW is disposed of in open air evaporation ponds. After evaporation of most of the water, OMWS is left in the bottom of the ponds. In this thesis, the effort has been made to evaluate the catalytic pyrolysis process as a technology to valorize the OMWS. The first section of this research showed that 41.12 wt. % of the OMWS is mostly lipids, which are a good source of energy. The second section proved that catalytic pyrolysis of the OMWS over red mud and HZSM-5 can produce green diesel, and 450 °C is the optimal reaction temperature to maximize the organic yields. The last section revealed that the HSF was behind the good fuel-like properties of the OMWS catalytic oils, whereas the SR hindered the bio-oil yields and quality.

Yield and composition of grape seed oils extracted by supercritical carbon dioxide and petroleum ether: varietal effects.

PubMed

Beveridge, Thomas H J; Girard, Benoit; Kopp, Thomas; Drover, John C G

2005-03-09

Grape seed has a well-known potential for production of oil as a byproduct of winemaking and is currently produced as a specialty oil byproduct of wine manufacture. Seed oils from eight varieties of grapes crushed for wine production in British Columbia were extracted by supercritical carbon dioxide (SCE) and petroleum ether (PE). Oil yields by SCE ranged from 5.85 +/- 0.33 to 13.6 +/- 0.46% (w/w), whereas PE yields ranged from 6.64 +/- 0.16 to 11.17 +/- 0.05% (+/- is standard deviation). The oils contained alpha-, beta-, and gamma-tocopherols and alpha- and gamma-tocotrienols, with gamma-tocotrienol being most important quantitatively. In both SCE- and PE-extracted oils, phytosterols were a prominent feature of the unsaponifiable fraction, with beta-sitosterol quantitatively most important with both extractants. Total phytosterol extraction was higher with SCE than with PE in seven of eight variety extractions. Fatty acid composition of oils from all varieties tested, and from both extraction methods, indicated linoleic acid as the major component ranging from 67.56 to 73.23% of the fatty acids present, in agreement with literature reports.

Alkaline hydrothermal liquefaction of swine carcasses to bio-oil.

PubMed

Zheng, Ji-Lu; Zhu, Ming-Qiang; Wu, Hai-tang

2015-09-01

It is imperative that swine carcasses are disposed of safely, practically and economically. Alkaline hydrothermal liquefaction of swine carcasses to bio-oil was performed. Firstly, the effects of temperature, reaction time and pH value on the yield of each liquefaction product were determined. Secondly, liquefaction products, including bio-oil and solid residue, were characterized. Finally, the energy recovery ratio (ERR), which was defined as the energy of the resultant products compared to the energy input of the material, was investigated. Our experiment shows that reaction time had certain influence on the yield of liquefaction products, but temperature and pH value had bigger influence on the yield of liquefaction products. Yields of 62.2wt% bio-oil, having a high heating value of 32.35MJ/kg and a viscosity of 305cp, and 22wt% solid residue were realized at a liquefaction temperature of 250°C, a reaction time of 60min and a pH value of 9.0. The bio-oil contained up to hundreds of different chemical components that may be classified according to functional groups. Typical compound classes in the bio-oil were hydrocarbons, organic acids, esters, ketones and heterocyclics. The energy recovery ratio (ERR) reached 93.63%. The bio-oil is expected to contribute to fossil fuel replacement in stationary applications, including boilers and furnaces, and upgrading processes for the bio-oil may be used to obtain liquid transport fuels. Copyright © 2015 Elsevier Ltd. All rights reserved.

Salt-induced modulation in inorganic nutrients, antioxidant enzymes, proline content and seed oil composition in safflower (Carthamus tinctorius L.).

PubMed

Siddiqi, Ejaz Hussain; Ashraf, Muhammad; Al-Qurainy, Fahad; Akram, Nudrat Aisha

2011-12-01

Safflower (Carthamus tinctorius L.) has gained considerable ground as a potential oil-seed crop. However, its yield and oil production are adversely affected under saline conditions. The present study was conducted to appraise the influence of salt (NaCl) stress on yield, accumulation of different inorganic elements, free proline and activities of some key antioxidant enzymes in plant tissues as well as seed oil components in safflower. Two safflower accessions differing in salt tolerance (Safflower-33 (salt sensitive) and Safflower-39 (salt tolerant)) were grown under saline (150 mmol L(-1) ) conditions and salt-induced changes in the earlier-mentioned physiological attributes were determined. Salt stress enhanced leaf and root Na(+) , Cl(-) and proline accumulation and activities of leaf superoxide dismutase, catalase and peroxidase, while it decreased K(+) , Ca(2+) and K(+) /Ca(2+) and Ca(2+) /Na(+) ratios and seed yield, 100-seed weight, number of seeds, as well as capitula, seed oil contents and oil palmitic acid. No significant effect of salt stress was observed on seed oil α-tocopherols, stearic acid, oleic acid or linoleic acid contents. Of the two safflower lines, salt-sensitive Safflower-33 was higher in leaf and root Na(+) and Cl(-) , while Safflower-39 was higher in leaf and root K(+) , K(+) /Ca(2+) and Ca(2+) /Na(+) and seed yield, 100-seed weight, catalase activity, seed oil contents, seed oil α-tocopherol and palmitic acid. Other attributes remained almost unaffected in both accessions. Overall, high salt tolerance of Safflower-39 could be attributed to Na(+) and Cl(-) exclusion, high accumulation of K(+) and free proline, enhanced CAT activity, seed oil α-tocopherols and palmitic acid contents. Copyright © 2011 Society of Chemical Industry.

Comparison of oil refining and biodiesel production process between screw press and n-hexane techniques from beauty leaf feedstock

NASA Astrophysics Data System (ADS)

Bhuiya, M. M. K.; Rasul, M. G.; Khan, M. M. K.; Ashwath, N.

2016-07-01

The Beauty Leaf Tree (Callophylum inophyllum) is regarded as an alternative source of energy to produce 2nd generation biodiesel due to its potentiality as well as high oil yield content in the seed kernels. The treating process is indispensable during the biodiesel production process because it can augment the yield as well as quality of the product. Oil extracted from both mechanical screw press and solvent extraction using n-hexane was refined. Five replications each of 25 gm of crude oil for screw press and five replications each of 25 gm of crude oil for n-hexane were selected for refining as well as biodiesel conversion processes. The oil refining processes consists of degumming, neutralization as well as dewaxing. The degumming, neutralization and dewaxing processes were performed to remove all the gums (phosphorous-based compounds), free fatty acids, and waxes from the fresh crude oil before the biodiesel conversion process carried out, respectively. The results indicated that up to 73% and 81% of mass conversion efficiency of the refined oil in the screw press and n-hexane refining processes were obtained, respectively. It was also found that up to 88% and 90% of biodiesel were yielded in terms of mass conversion efficiency in the transesterification process for the screw press and n-hexane techniques, respectively. While the entire processes (refining and transesterification) were considered, the conversion of beauty leaf tree (BLT) refined oil into biodiesel was yielded up to 65% and 73% of mass conversion efficiency for the screw press and n-hexane techniques, respectively. Physico-chemical properties of crude and refined oil, and biodiesel were characterized according to the ASTM standards. Overall, BLT has the potential to contribute as an alternative energy source because of high mass conversion efficiency.

Transesterification of rapeseed oil for biodiesel production in trickle-bed reactors packed with heterogeneous Ca/Al composite oxide-based alkaline catalyst.

PubMed

Meng, Yong-Lu; Tian, Song-Jiang; Li, Shu-Fen; Wang, Bo-Yang; Zhang, Min-Hua

2013-05-01

A conventional trickle bed reactor and its modified type both packed with Ca/Al composite oxide-based alkaline catalysts were studied for biodiesel production by transesterification of rapeseed oil and methanol. The effects of the methanol usage and oil flow rate on the FAME yield were investigated under the normal pressure and methanol boiling state. The oil flow rate had a significant effect on the FAME yield for the both reactors. The modified trickle bed reactor kept over 94.5% FAME yield under 0.6 mL/min oil flow rate and 91 mL catalyst bed volume, showing a much higher conversion and operational stability than the conventional type. With the modified trickle bed reactor, both transesterification and methanol separation could be performed simultaneously, and glycerin and methyl esters were separated additionally by gravity separation. Copyright © 2013 Elsevier Ltd. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Joyce, Blake L.; Zheljazkov, Valtcho D.; Sykes, Robert

Cymbopogon flexuosus, lemongrass, and C. martinii, palmarosa, are perennial grasses grown to produce essential oils for the fragrance industry. The objectives of this study were (1) to evaluate biomass and oil yields as a function of nitrogen and sulfur fertilization, and (2) to characterize their utility for lignocellulosic ethanol compared to Panicum virgatum (switchgrass). Mean biomass yields were 12.83 Mg lemongrass ha -1 and 15.11 Mg palmarosa ha -1 during the second harvest year resulting in theoretical biofuel yields of 2541 and 2569 L ethanol ha -1 respectively compared to reported 1749–3691 L ethanol ha -1 for switchgrass. Pretreated lemongrassmore » yielded 198 mL ethanol (g biomass) -1 and pretreated palmarosa yielded 170 mL ethanol (g biomass) -1. Additionally, lemongrass yielded 85.7 kg essential oil ha-1 and palmarosa yielded 67.0 kg ha -1 with an estimated value of USD $857 and $1005 ha -1. These data suggest that dual-use crops such as lemongrass and palmarosa may increase the economic viability of lignocellulosic biofuels.« less

Effects of ambient and elevated level of ozone on Brassica campestris L. with special reference to yield and oil quality parameters.

PubMed

Tripathi, Ruchika; Agrawal, S B

2012-11-01

Tropospheric ozone (O(3)) has become a serious threat to growth and yield of important agricultural crops over Asian regions including India. Effect of elevated O(3) (ambient+10ppb) was studied on Brassica campestris L. (cv. Sanjukta and Vardan) in open top chambers under natural field conditions. Eight hourly mean ambient O(3) concentration varied from 26.3ppb to 69.5ppb during the growth period. Plants under O(3) exposure showed reductions in photosynthetic rate, reproductive parameters, yield as well as seed and oil quality. Cultivar Sanjukta showed more reduction in photosynthetic characteristics, reproductive structures and seed and oil quality. However, total yield was more affected in Vardan. Exposure of O(3) increased the degree of unsaturation and level of PUFA, ω-6fatty acid, linolenic acid and erucic acid in oil indicating the deterioration of its quality. The study further confirmed that there is a correspondence between O(3) induced change in photosynthetic processes, reproductive development and yield and did not find any compensatory response in the final yield. Copyright © 2012 Elsevier Inc. All rights reserved.

Autocatalytic Pyrolysis of Wastewater Biosolids for Product Upgrading.

PubMed

Liu, Zhongzhe; McNamara, Patrick; Zitomer, Daniel

2017-09-05

The main goals for sustainable water resource recovery include maximizing energy generation, minimizing adverse environmental impacts, and recovering beneficial resources. Wastewater biosolids pyrolysis is a promising technology that could help facilities reach these goals because it produces biochar that is a valuable soil amendment as well as bio-oil and pyrolysis gas (py-gas) that can be used for energy. The raw bio-oil, however, is corrosive; therefore, employing it as fuel is challenging using standard equipment. A novel pyrolysis process using wastewater biosolids-derived biochar (WB-biochar) as a catalyst was investigated to decrease bio-oil and increase py-gas yield for easier energy recovery. WB-biochar catalyst increased the py-gas yield nearly 2-fold, while decreasing bio-oil production. The catalyzed bio-oil also contained fewer constituents based on GC-MS and GC-FID analyses. The energy shifted from bio-oil to py-gas, indicating the potential for easier on-site energy recovery using the relatively clean py-gas. The metals contained in wastewater biosolids played an important role in upgrading pyrolysis products. The Ca and Fe in WB-biochar reduced bio-oil yield and increased py-gas yield. The py-gas energy increase may be especially useful at water resource recovery facilities that already combust anaerobic digester biogas for energy since it may be possible to blend biogas and py-gas for combined use.

Effect of Nb on microstructure and yield strength of a high temperature tempered martensitic steel

NASA Astrophysics Data System (ADS)

Wang, Qian; Sun, Yu; Zhang, Chuanyou; Wang, Qingfeng; Zhang, Fucheng

2018-04-01

Martensitic steels based on a composition of 25CrMo47NbVTi with different concentrations of Nb (0.003%–0.060%) were quenched (Q) at 900 °C and tempered (T) at 700 °C to obtain oil country tubular goods (OCTG) with higher yield strength. The precipitation and microstructures were characterized and quantified by optical microscopy (OM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and x-ray diffraction (XRD). The results show that the increased Nb content led to an enhanced overall precipitation, the rising solution-precipitation temperature, the increased mass or volume fraction of the Nb-containing precipitates, and the decreased average diameter of Nb-containing particles. With the enhanced precipitation of small sized Nb-containing particles, the austenite grain and corresponding martensitic packet and block were evidently refined. In addition, the dislocation density increased slightly with increasing Nb addition. The yield strength was experimentally measured and quantitatively estimated. The findings based on theoretical calculations indicated that as a consequence of intensified strengthening from grain boundaries, precipitates and dislocations, the yield strength was enhanced significantly by Nb addition.

Effective depolymerization of concentrated acid hydrolysis lignin using a carbon-supported ruthenium catalyst in ethanol/formic acid media.

PubMed

Kristianto, Ivan; Limarta, Susan Olivia; Lee, Hyunjoo; Ha, Jeong-Myeong; Suh, Dong Jin; Jae, Jungho

2017-06-01

Lignin isolated by two-step concentrated acid hydrolysis of empty fruit bunch (EFB) was effectively depolymerized into a high-quality bio-oil using formic acid (FA) as an in-situ hydrogen source and Ru/C as a catalyst in supercritical ethanol. A bio-oil yield of 66.3wt% with an average molecular weight of 822g/mol and an aromatic monomer content of 6.1wt% was achieved at 350°C and a FA-to-lignin mass ratio of 3 after a reaction time of 60min. The combination of Ru/C and FA also resulted in a significant reduction in the oxygen content of the bio-oil by ∼60% and a corresponding increase in the higher heating value (HHV) to 32.7MJ/kg due to the enhanced hydrodeoxygenation activity. An examination of the FA decomposition characteristics revealed that Ru/C provides a greater increase in the rate of hydrogen production from FA, explaining the efficient depolymerization of lignin in a combined system. Copyright © 2017 Elsevier Ltd. All rights reserved.

Alkaline hydrothermal liquefaction of swine carcasses to bio-oil

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zheng, Ji-Lu, E-mail: triace@163.com; Zhu, Ming-Qiang; Wu, Hai-tang

Highlights: • Swine carcasses can be converted to bio-oil by alkaline hydrothermal liquefaction. • It seems that the use of the bio-oil for heat or CHP is technically suitable. • Some valuable chemicals were found in the bio-oils. • The bio-oil and the solid residue constituted an energy efficiency of 93.63% for the feedstock. • The solid residue can be used as a soil amendment, to sequester C and for preparing activated carbon. - Abstract: It is imperative that swine carcasses are disposed of safely, practically and economically. Alkaline hydrothermal liquefaction of swine carcasses to bio-oil was performed. Firstly, themore » effects of temperature, reaction time and pH value on the yield of each liquefaction product were determined. Secondly, liquefaction products, including bio-oil and solid residue, were characterized. Finally, the energy recovery ratio (ERR), which was defined as the energy of the resultant products compared to the energy input of the material, was investigated. Our experiment shows that reaction time had certain influence on the yield of liquefaction products, but temperature and pH value had bigger influence on the yield of liquefaction products. Yields of 62.2 wt% bio-oil, having a high heating value of 32.35 MJ/kg and a viscosity of 305cp, and 22 wt% solid residue were realized at a liquefaction temperature of 250 °C, a reaction time of 60 min and a pH value of 9.0. The bio-oil contained up to hundreds of different chemical components that may be classified according to functional groups. Typical compound classes in the bio-oil were hydrocarbons, organic acids, esters, ketones and heterocyclics. The energy recovery ratio (ERR) reached 93.63%. The bio-oil is expected to contribute to fossil fuel replacement in stationary applications, including boilers and furnaces, and upgrading processes for the bio-oil may be used to obtain liquid transport fuels.« less

Techno-economic and resource analysis of hydroprocessed renewable jet fuel.

PubMed

Tao, Ling; Milbrandt, Anelia; Zhang, Yanan; Wang, Wei-Cheng

2017-01-01

Biomass-derived jet fuel is an alternative jet fuel (AJF) showing promise of reducing the dependence on fossil fuel and greenhouse gas emissions. Hydroprocessed esters and fatty acids (HEFA) concept is also known as one of the pathways for producing bio jet fuel. HEFA fuel was approved by the American Society for Testing and Materials in 2011, and can be blended up to 50% with conventional jet fuel. Since then, several HEFA economic and life-cycle assessments have been published in literature. However, there have been limited analyses on feedstock availability, composition, and their impact on hydrocarbon yield (particularly jet blendstock yield) and overall process economics. This study examines over 20 oil feedstocks, their geographic distribution and production levels, oil yield, prices, and chemical composition. The results of our compositional analysis indicate that most oils contain mainly C 16 and C 18 fatty acids except pennycress, yellow grease, and mustard, which contain higher values and thus would require hydrocracking to improve jet fuel production. Coconut oil has a large content of shorter carbon fatty acids, making it a good feedstock candidate for renewable gasoline instead of jet substitutes' production. Techno-economic analysis (TEA) was performed for five selected oil feedstocks-camelina, pennycress, jatropha, castor bean, and yellow grease-using the HEFA process concept. The resource analysis indicates that oil crops currently grown in the United States (namely soybean) have relatively low oil yield when compared to oil crops grown in other parts of the world, such as palm, coconut, and jatropha. Also, non-terrestrial oil sources, such as animal fats and greases, have relatively lower prices than terrestrial oil crops. The minimum jet fuel selling price for these five resources ranges between $3.8 and $11.0 per gallon. The results of our TEA and resource studies indicate the key cost drivers for a biorefinery converting oil to jet hydrocarbons are as follows: oil price, conversion plant capacity, fatty acid profile, addition of hydrocracker, and type of hydroprocessing catalysts.

Microcapsules with a pH responsive polymer: influence of the encapsulated oil on the capsule morphology.

PubMed

Wagdare, Nagesh A; Marcelis, Antonius T M; Boom, Remko M; van Rijn, Cees J M

2011-11-01

Microcapsules were prepared by microsieve membrane cross flow emulsification of Eudragit FS 30D/dichloromethane/edible oil mixtures in water, and subsequent phase separation induced by extraction of the dichloromethane through an aqueous phase. For long-chain triglycerides and jojoba oil, core-shell particles were obtained with the oil as core, surrounded by a shell of Eudragit. Medium chain triglyceride (MCT oil) was encapsulated as relatively small droplets in the Eudragit matrix. The morphology of the formed capsules was investigated with optical and SEM microscopy. Extraction of the oil from the core-shell capsules with hexane resulted in hollow Eudragit capsules with porous shells. It was shown that the differences are related to the compatibility of the oils with the shell-forming Eudragit. An oil with poor compatibility yields microcapsules with a dense Eudragit shell on a single oil droplet as the core; oils having better compatibility yield porous Eudragit spheres with several oil droplets trapped inside. Copyright © 2011 Elsevier B.V. All rights reserved.

Effect of Reinforcement Shape and Fiber Treatment on the Mechanical Properties of Oil Palm Empty Fruit Bunch-Polyethylene Composites

DOE Office of Scientific and Technical Information (OSTI.GOV)

Arif, M. F.; Yusoff, P. S. M. M.; Eng, K. K.

2010-03-11

High Density Polyethylene (HDPE) composites were fabricated using oil palm empty fruit bunch (EFB) as the reinforcing material. The effect of reinforcement shape on the tensile and flexural properties, that is 5 mm average length of short fiber and 325-400 {mu}m size distribution of particulate filler have been studied. Overall, EFB short fiber-HDPE composites yield higher mechanical properties compared to EFB particulate-HDPE composites. For both types of composites, considerable improvement showed in tensile and flexural modulus. However, the tensile strength decreased with increase in EFB content. Attempts to improve these properties using alkali and two types of silane, namely gamma-Methacryloxypropyltrimethoxysilanemore » (MTS) and vinyltriethoxysilane (VTS) were described. It is found that both types of silane enhanced the mechanical properties of composites. MTS showed better tensile strength compared to VTS. However, only marginal improvement obtained from alkali treatments.« less

Climate impacts on palm oil yields in the Nigerian Niger Delta

NASA Astrophysics Data System (ADS)

Okoro, Stanley U.; Schickhoff, Udo; Boehner, Juergen; Schneider, Uwe A.; Huth, Neil

2016-04-01

Palm oil production has increased in recent decades and is estimated to increase further. The optimal role of palm oil production, however, is controversial because of resource conflicts with alternative land uses. Local conditions and climate change affect resource competition and the desirability of palm oil production. Based on this, crop yield simulations using different climate model output under different climate scenarios could be important tool in addressing the problem of uncertainty quantification among different climate model outputs. Previous studies on this region have focused mostly on single experimental fields, not considering variations in Agro-Ecological Zones, climatic conditions, varieties and management practices and, in most cases not extending to various IPCC climate scenarios and were mostly based on single climate model output. Furthermore, the uncertainty quantification of the climate- impact model has rarely been investigated on this region. To this end we use the biophysical simulation model APSIM (Agricultural Production Systems Simulator) to simulate the regional climate impact on oil palm yield over the Nigerian Niger Delta. We also examine whether the use of crop yield model output ensemble reduces the uncertainty rather than the use of climate model output ensemble. The results could serve as a baseline for policy makers in this region in understanding the interaction between potentials of energy crop production of the region as well as its food security and other negative feedbacks that could be associated with bioenergy from oil palm. Keywords: Climate Change, Climate impacts, Land use and Crop yields.

Process optimisation of microwave-assisted extraction of peony ( Paeonia suffruticosa Andr .) seed oil using hexane-ethanol mixture and its characterisation

Treesearch

Xiaoli Sun; Wengang Li; Jian Li; Yuangang Zu; Chung-Yun Hse; Jiulong Xie; Xiuhua Zhao

2016-01-01

Ethanol and hexane mixture agent microwave-assisted extraction (MAE) method was conducted to extract peony (Paeonia suffruticosa Andr.) seed oil (PSO). The aim of the study was to optimise the extraction for both yield and energy consumption in mixture agent MAE. The highest oil yield (34.49%) and lowest unit energy consumption (14 125.4 J g -1)...

Optimisation of Croton gratissimus Oil Extraction by n-Hexane and Ethyl Acetate Using Response Surface Methodology.

PubMed

Jiyane, Phiwe Charles; Tumba, Kaniki; Musonge, Paul

2018-04-01

The extraction of oil from Croton gratissimus seeds was studied using the three-factor five-level full-factorial central composite rotatable design (CCRD) of the response surface methodology (RSM). The effect of the three factors selected, viz., extraction time, extraction temperature and solvent-to-feed ratio on the extraction oil yield was investigated when n-hexane and ethyl acetate were used as extraction solvents. The coefficients of determination (R 2 ) of the models developed were 0.98 for n-hexane extraction and 0.97 for ethyl acetate extraction. These results demonstrated that the models developed adequately represented the processes they described. From the optimized model, maximum extraction yield obtained from n-hexane and ethyl acetate extraction were 23.88% and 23.25%, respectively. In both cases the extraction temperature and solvent-to-feed ratio were 35°C and 5 mL/g, respectively. In n-hexane extraction the maximum conditions were reached only after 6 min whereas in ethyl acetate extraction it took 20 min to get the maximum extraction oil yield. Oil extraction of Croton gratissimus seeds, in this work, favoured the use of n-hexane as an extraction solvent as it offered higher oil yields at low temperatures and reduced residence times.

Chemically inducing lightwood formation in southern pines

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roberts, D.R.; Peters, W.J.

1977-06-01

Chemical induction of lightwood formation promises to be a new method of naval stores production. A broad range of paraquat concentrations and many methods of application induced lightwood formation. Loblolly, slash, and longleaf pines were found to produce increased amounts of turpentine and tall oil in response to paraquat treatment. In one experiment, loblolly pines treated with 8 percent paraquat on a single bark streak yielded, 9 months after treatment, an average of 10 pounds more extractives per tree than did untreated trees. Most of the yield increase was in the lower portion of the tree near the wound, butmore » some increase was noted for heights as great as 27 ft. In another experiment, 8 to 10 inch DBH loblolly, slash, and longleaf pine trees were treated with 0.5, 1.0, and 2.5 percent paraquat applied in ax chops spanning one-third the circumference of the trees. All treated trees yielded more resin acids than did control trees.« less

Effect of chronic oil pollution on salt-marsh nitrogen fixation (acetylene redution). [Spartina alterniflora

DOE Office of Scientific and Technical Information (OSTI.GOV)

Thomson, A.D.; Webb, K.L.

1984-03-01

Annual acetylene reduction rates associated with intertidal communities in a chronically oil polluted Virgina salt marsh were compared to rates measured in an undisturbed marsh. Chronic oil treatment resulted in visible damage to the higher plants of the Spartina alterniflora zones; however, vegetation-associated acetylene reduction was not different from the untreated control. Sediment rates generally were affected little by oil application, except during the summer when rates in the median tidal elevation zones were considerably higher than those of the control. Acetylene reduction occurred in all transects, each of which extended from upper mudflat to the Spartina patens zone. Intertidalmore » sediment acetylene reduction was patchy, both spatially and seasonally. Estimated rates were greatest near the surface; free-living bacterial N/sub 2/ fixation activity averaged 2.23 mg N per m/sup 2/ per d (range = undetectable to 365 mg N per m/sup 2/ per d) in the untreated and 3.17 mg N per m/sup 2/ per d (range = undetectable to 564 mg N per m/sup 2/ per d) in the oil-treated marsh during the year. Vegetation-associated N/sub 2/ fixation activity yielded highest overall mean rates (156 mg N per M/sub 2/ per d). The seasonal pattern of sediment and vegetation-associated fixation may be controlled by temperature and availability of oxidizable substrates. 39 references, 2 figures, 5 tables.« less

Surface-water characteristics and quality on the Osage Reservation, Osage County, Oklahoma, 1999

USGS Publications Warehouse

Abbott, Marvin M.; Tortorelli, Robert L.

2002-01-01

Concern about the effects of early oil-industry practices of surface disposal of produced-brine water prompted an investigation of the surface-water quality on the Osage Reservation. About 38,600 oil wells have been drilled on the Osage Reservation since drilling began in 1896. The Osage Reservation comprises three major drainage basins. The Caney River Basin is in the northeast, the Bird Creek Basin is in the southeast, and the Salt Creek Basin in the west. Variations in streamflow on the Osage Reservation during a year primarily result from variations in the quantity and frequency of rainfall, evapotranspiration, and reservoir operations. Most streams do not flow during low rainfall periods in late summer, early fall, and in winter. Percent of mean annual discharge is largest during March through June, averaging 54 to 62 percent and smallest during December, January, July, and August, averaging only 14 to 21 percent. The basin areas of Caney River in the reservation (251 square miles), Salt Creek (273 square miles), and Sand Creek (227 square miles) are about the same and the basin areas of the Bird Creek Basin (418 square miles) and Homily Creek Basin (383 square miles) are similar in area. One hundred forty surface-water sites were sampled once during either February, March or August 1999. The surface-drainage areas, incremental basins, between sample sites along a stream, range in size from 0.26 to 123 square miles with a median of 8.6 square miles. Total number of oil wells upgradient of the samples sites is 31,432 or 80 percent of the total in the reservation. The total number of oil wells in the Caney River Basin in the reservation (2,975 wells), Salt Creek Basin (4,619 wells), and Sand Creek Basin (3,858 wells) are about the same and the total number of oil wells in the Bird Creek Basin (8,858 wells) and Hominy Creek Basin (7,842 wells) are similar. The number of oil wells per square mile in the incremental basins ranges for 0.86 to 154. Surface-water quality monitoring had been conducted previously at two sites included in this study. Dissolved chloride concentrations for the two samples collected during 1999 were equaled or exceeded at both sites by the historical data. There is no statistically significant difference between the distribution of the dissolved chloride concentrations from the surface water and nearby ground-water samples. The surface-water quality samples had significantly lesser concentrations of dissolved solids, sulfate, and nitrite plus nitrate as nitrogen than the ground-water samples. Chloride yield, reported in tons per day per square mile, is the chloride load divided by the basin area upstream of the sample site. The mean of the chloride yields for all the samples was 0.07 ton per day per square mile. Many sample locations where yields were greater than 0.07 ton per day per square mile were areas where dissolved chloride concentrations from surface-water samples were greater than 250 milligrams per liter in an earlier water-quality investigation. An investigation of possible relations between the surface-water quality data and the oil-well construction data for the incremental basins and for 1-mile radial distance upstream in the incremental basins was conducted. The oil-well data also were grouped by the time periods of activity into pre-1930, 1930 to 1970, and post-1970. These groups attempt to account for differences in industry drilling and producing practices associated with various periods. No statistically significant correlations were found between the surface-water quality data and the oil-well construction data.

Geology, thermal maturation, and source rock geochemistry in a volcanic covered basin: San Juan sag, south-central Colorado

USGS Publications Warehouse

Gries, R.R.; Clayton, J.L.; Leonard, C.

1997-01-01

The San Juan sag, concealed by the vast San Juan volcanic field of south-central Colorado, has only recently benefited from oil and gas wildcat drilling and evaluations. Sound geochemical analyses and maturation modeling are essential elements for successful exploration and development. Oil has been produced in minor quantities from an Oligocene sill in the Mancos Shale within the sag, and major oil and gas production occurs from stratigraphically equivalent rocks in the San Juan basin to the south-west and in the Denver basin to the northeast. The objectives of this study were to identify potential source rocks, assess thermal maturity, and determine hydrocarbon-source bed relationships. Source rocks are present in the San Juan sag in the upper and lower Mancos Shale (including the Niobrara Member), which consists of about 666 m (2184 ft) of marine shale with from 0.5 to 3.1 wt. % organic carbon. Pyrolysis yields (S1 + S2 = 2000-6000 ppm) and solvent extraction yields (1000-4000 ppm) indicate that some intervals within the Mancos Shale are good potential source rocks for oil, containing type II organic matter, according to Rock-Eval pyrolysis assay. Oils produced from the San Juan sag and adjacent part of the San Juan basin are geochemically similar to rock extracts obtained from these potential source rock intervals. Based on reconstruction of the geologic history of the basin integrated with models of organic maturation, we conclude that most of the source rock maturation occurred in the Oligocene and Miocene. Little to no maturation took place during Laramide subsidence of the basin, when the Animas and Blanco Basin formations were deposited. The timing of maturation is unlike that of most Laramide basins in the Rocky Mountain region, where maturation occurred as a result of Paleocene and Eocene basin fill. The present geothermal gradient in the San Juan sag is slightly higher (average 3.5??C/100 m; 1.9??F/100 ft) than the regional average for southern Rocky Mountain basins; however, although the sag contains intrusives and a volcanic cover, the gradient is significantly lower than that reported for parts of the adjacent San Juan basin (4.7??C/100 m; 2.6??F/100 ft). Burial depth appears to be a more important controlling factor in the thermal history of the source rocks than local variations in the geothermal gradient due to volcanic activity. Interestingly, the thick overburden of volcanic rocks appears to have provided the necessary burial depth for maturation.

Geochemical analysis of potash mine seep oils, collapsed breccia pipe oil shows and selected crude oils, Eddy County, New Mexico

USGS Publications Warehouse

Palacas, J.G.; Snyder, R.P.; Baysinger, J.P.; Threlkeld, C.N.

1982-01-01

Oil shows, in the form of oil stains and bleeding oil, in core samples from two breccia pipes, Hills A and C, Eddy County, New Mexico, and seepage oils in a potash mine near Hill C breccia pipe are geochemically similar. The geochemical similarities strongly suggest that they belong to the same family of oils and were derived from similar sources. The oils are relatively high in sulfur (0.89 to 1.23 percent), rich in hydrocarbons (average 82 percent), relatively high in saturated hydrocarbon/aromatic hydrocarbon ratios (average 2.9), and based on analysis of seep oils alone, have a low API gravity (average 19.4?). The oils are for the most part severely biodegraded as attested by the loss of n-paraffin molecules. Geochemical comparison of seven crude oils collected in the vicinity of the breccia pipes indicates that the Yates oils are the likely source of the above family of oils. Six barrels of crude oil that were dumped into a potash exploration borehole near Hill C breccia pipe, to release stuck casing, are considered an unlikely source of the breccia pipe and mine seep oils. Volumetric and hydrodynamic constraints make it highly improbable that such a small volume of 'dumped' oil could migrate over distances ranging from about 600 feet to 2.5 miles to the sites of the oil shows.

Soy Sauce Residue Oil Extracted by a Novel Continuous Phase Transition Extraction under Low Temperature and Its Refining Process.

PubMed

Zhao, Lichao; Zhang, Yong; He, Liping; Dai, Weijie; Lai, Yingyi; Yao, Xueyi; Cao, Yong

2014-04-09

On the basis of previous single-factor experiments, extraction parameters of soy sauce residue (SSR) oil extracted using a self-developed continuous phase transition extraction method at low temperature was optimized using the response surface methodology. The established optimal conditions for maximum oil yield were n-butane solvent, 0.5 MPa extraction pressure, 45 °C temperature, 62 min extraction time, and 45 mesh raw material granularity. Under these conditions, the actual yield was 28.43% ± 0.17%, which is relatively close to the predicted yield. Meanwhile, isoflavone was extracted from defatted SSR using the same method, but the parameters and solvent used were altered. The new solvent was 95% (v/v) ethanol, and extraction was performed under 1.0 MPa at 60 °C for 90 min. The extracted isoflavones, with 0.18% ± 0.012% yield, mainly comprised daidzein and genistein, two kinds of aglycones. The novel continuous phase transition extraction under low temperature could provide favorable conditions for the extraction of nonpolar or strongly polar substances. The oil physicochemical properties and fatty acids compositions were analyzed. Results showed that the main drawback of the crude oil was the excess of acid value (AV, 63.9 ± 0.1 mg KOH/g) and peroxide value (POV, 9.05 ± 0.3 mmol/kg), compared with that of normal soybean oil. However, through molecular distillation, AV and POV dropped to 1.78 ± 0.12 mg KOH/g and 5.9 ± 0.08 mmol/kg, respectively. This refined oil may be used as feedstuff oil.

Incubation of Aquilaria subintegra with Microbial Culture Supernatants Enhances Production of Volatile Compounds and Improves Quality of Agarwood Oil.

PubMed

Monggoot, Sakon; Kulsing, Chadin; Wong, Yong Foo; Pripdeevech, Patcharee

2018-06-01

Incubation with microbial culture supernatants improved essential oil yield from Aquilaria subintegra woodchips. The harvested woodchips were incubated with de man, rogosa and sharpe (MRS) agar, yeast mold (YM) agar medium and six different microbial culture supernatants obtained from Lactobacillus bulgaricus , L. acidophilus , Streptococcus thermophilus , Lactococcus lactis , Saccharomyces carlsbergensis and S. cerevisiae prior to hydrodistillation. Incubation with lactic acid bacteria supernatants provided higher yield of agarwood oil (0.45% w/w) than that obtained from yeast (0.25% w/w), agar media (0.23% w/w) and water (0.22% w/w). The composition of agarwood oil from all media and microbial supernatant incubations was investigated by using gas chromatography-mass spectrometry. Overall, three major volatile profiles were obtained, which corresponded to water soaking (control), as well as, both YM and MRS media, lactic acid bacteria, and yeast supernatant incubations. Sesquiterpenes and their oxygenated derivatives were key components of agarwood oil. Fifty-two volatile components were tentatively identified in all samples. Beta-agarofuran, α-eudesmol, karanone, α-agarofuran and agarospirol were major components present in most of the incubated samples, while S. cerevisiae -incubated A. subintegra provided higher amount of phenyl acetaldehyde. Microbial culture supernatant incubation numerically provided the highest yield of agarwood oil compared to water soaking traditional method, possibly resulting from activity of extracellular enzymes produced by the microbes. Incubation of agarwood with lactic acid bacteria supernatant significantly enhanced oil yields without changing volatile profile/composition of agarwood essential oil, thus this is a promising method for future use.

Yield and Water Productivity Responses to Irrigation Cut-off Strategies after Fruit Set Using Stem Water Potential Thresholds in a Super-High Density Olive Orchard

PubMed Central

Ahumada-Orellana, Luis E.; Ortega-Farías, Samuel; Searles, Peter S.; Retamales, Jorge B.

2017-01-01

An increase in the land area dedicated to super-high density olive orchards has occurred in Chile in recent years. Such modern orchards have high irrigation requirements, and optimizing water use is a priority. Moreover, this region presents low water availability, which makes necessary to establish irrigation strategies to improve water productivity. An experiment was conducted during four consecutive growing seasons (2010–2011 to 2013–2014) to evaluate the responses of yield and water productivity to irrigation cut-off strategies. These strategies were applied after fruit set using midday stem water potential (Ψstem) thresholds in a super-high density olive orchard (cv. Arbequina), located in the Pencahue Valley, Maule Region, Chile. The experimental design was completely randomized with four irrigation cut-off treatments based on the Ψstem thresholds and four replicate plots per treatment (five trees per plot). Similar to commercial growing conditions in our region, the Ψstem in the T1 treatment was maintained between -1.4 and -2.2 MPa (100% of actual evapotranspiration), while T2, T3 and T4 treatments did not receive irrigation from fruit set until they reached a Ψstem threshold of approximately -3.5, -5.0, and -6.0 MPa, respectively. Once the specific thresholds were reached, irrigation was restored and maintained as T1 in all treatments until fruits were harvested. Yield and its components were not significantly different between T1 and T2, but fruit yield and total oil yield, fruit weight, and fruit diameter were decreased by the T3 and T4 treatments. Moreover, yield showed a linear response with water stress integral (SΨ), which was strongly influenced by fruit load. Total oil content (%) and pulp/stone ratio were not affected by the different irrigation strategies. Also, fruit and oil water productivities were significantly greater in T1 and T2 than in the T3 and T4. Moreover, the T2, T3, and T4 treatments averaged 37, 51, and 72 days without irrigation which represented 75–83, 62–76, and 56–70% of applied water compared with T1, respectively. These results suggest that using the T2 irrigation cut-off strategy could be applied in a super-high density olive orchard (cv. Arbequina) because it maintained yields, saving 20% of the applied water. PMID:28785274

Trading forests for yields in the Peruvian Amazon

NASA Astrophysics Data System (ADS)

Gibbs, Holly

2012-03-01

Our knowledge of how agriculture expands, and the types of land it replaces, is remarkably limited across the tropics. Most remote-sensing studies focus on the net gains and losses in forests and agricultural land rather than the land-use transition pathways (Gibbs et al 2010). Only a handful of studies identify land sources for new croplands or plantations, and then only for farming systems aggregated together (e.g., Koh and Wilcove 2008, Morton et al 2006, Gibbs et al 2010). Gutiérrez-Vélez et al (2011), however, have taken a leap forward by tracking the different expansion pathways for smallholder and industrial oil palm plantations. Using a combination of Landsat, MODIS and field surveys, they investigate whether higher yields in new agricultural lands spare forests in the Peruvian Amazon and in a smaller focus area in the Ucayali region. Across the Peruvian Amazon, they show that between 2000 and 2010, new high-yield oil palm plantations replaced forests 72% of the time and accounted for 1.3% of total deforestation, with most expansion occurring after 2006. Gutiérrez-Vélez et al went further in the Ucayali region and compared land sources for new high-yield and low-yield plantations. Expansion of higher-yield agricultural lands should logically reduce the total area needed for production, thus potentially sparing forests. In the Ucayali focus area, expansion of high-yield oil palm did convert less total land area but more forest was cleared than with low-yield expansion. Smaller-scale plantations tended to expand into already cleared areas while industrial-scale plantations traded their greater yields for forests, leading to higher land-clearing carbon emissions per production unit (Gibbs et al 2008). Gutiérrez-Vélez et al show that higher yields may require less land for production but more forest may be lost in the process, and they emphasize the need for stronger incentives for land sparing. The potential land-saving nature of these high-yield plantations could be further analyzed by considering whether they help depress global prices, reducing incentives to expand elsewhere (Angelsen and Kaimowitz 2001). The significance of the study goes well beyond the bounds of Ucayli, and highlights risks to Amazonian forests from oil palm expansion (Butler and Laurance 2010). Oil palm is an astoundingly profitable and productive crop, with typical oil yields more than ten times that of soy. Some have even argued that oil palm is innately land sparing because it would take substantially more land for all other oil-bearing crops to provide the same output. However, most production gains from oil palm have occurred through increased area rather than increased yield, and in many cases expansion has been through forest clearing (Koh and Wilcove 2008, Gibbs et al 2010). The findings of Gutiérrez-Vélez et al (2011) are particularly significant considering that the booming palm oil sectors in Indonesia and Malaysia, which currently produce over 80% of the world's product, are facing a host of pressures that constrain future area expansion. Malaysia has little remaining land suited for plantations and Indonesia faces intensifying international scrutiny over the future of their forestlands. Consequently, the Amazon basin is widely considered the new frontier, with more than half of its forest area suitable for palm oil cultivation (Butler and Laurance 2010) and growing incentives from Brazil's Program for the Sustainable Production of Oil Palm, which aims to utilize degraded lands and spur reforestation efforts. Their results also illuminate another key issue, namely the constraints faced by large-scale producers when they seek to expand plantation area. Emerging demand-side conservation efforts, such as the Roundtable for Sustainable Palm Oil (RSPO), assume that already cleared and non-forested lands are freely available. Gutiérrez-Vélez et al (2011) hint at the obstacles to using such cleared lands, which is that they are inhabited and often have contested land tenure. We must carefully consider our consumption of these commodities in the face of growing land scarcity (Lambin and Meyfroidt 2011). If high-yield plantations displace low-yield plantations they too may follow the path of industrial agriculture and resume destruction of the forests that conservation efforts aim to protect. Without clear incentives to spare land, we could be trading forest for higher yields. References Angelsen A and Kaimowitz D 2001 Agricultural Technologies and Tropical Deforestation (New York: CABI Publishing) (www.cifor.org/publications/pdf files/books/bangelsen0101e0.pdf) Butler R and Laurance W 2010 Is oil palm the next emerging threat to the Amazon? Trop. Conserv. Sci. 2 1-10 Gibbs H K, Johnston M, Foley J A, Holloway T, Monfreda C, Ramankutty N and Zaks D 2008 Carbon payback times for crop-based biofuel expansion in the tropics: the effects of changing yield and technology Environ. Res. Lett. 3 034001 Gibbs H K, Ruesch A S, Achard F, Clayton M K, Holmgren P, Ramankutty N and Foley J A 2010 Tropical forests were the primary sources of new agricultural land in the 1980s and 1990s Proc. Natl Acad. Sci. 107 16732-7 Gutiérrez-Vélez V H, DeFries R, Pinedo-Vásquez M, Uriarte M, Padoch C, Baethgen W, Fernandes K and Lim Y 2011 High-yield oil palm expansion spares land at the expense of forests in the Peruvian Amazon Environ. Res. Lett. 6 044029 Koh L and Wilcove D 2008 Is oil palm agriculture really destroying tropical biodiversity? Conserv. Lett. 1 60-4 Lambin E and Meyfroidt P 2011 Inaugural article: global land use change, economic globalization, and the looming land scarcity Proc. Natl Acad. Sci. 108 93465-72 Morton D C et al 2006 Cropland expansion changes deforestation dynamics in the southern Brazilian Amazon Proc. Natl Acad. Sci. USA 103 14637-41

Ultrasound-assisted extraction (UAE) and solvent extraction of papaya seed oil: yield, fatty acid composition and triacylglycerol profile.

PubMed

Samaram, Shadi; Mirhosseini, Hamed; Tan, Chin Ping; Ghazali, Hasanah Mohd

2013-10-10

The main objective of the current work was to evaluate the suitability of ultrasound-assisted extraction (UAE) for the recovery of oil from papaya seed as compared to conventional extraction techniques (i.e., Soxhlet extraction (SXE) and solvent extraction (SE)). In the present study, the recovery yield, fatty acid composition and triacylglycerol profile of papaya seed oil obtained from different extraction methods and conditions were compared. Results indicated that both solvent extraction (SE, 12 h/25 °C) and ultrasound-assisted extraction (UAE) methods recovered relatively high yields (79.1% and 76.1% of total oil content, respectively). Analysis of fatty acid composition revealed that the predominant fatty acids in papaya seed oil were oleic (18:1, 70.5%-74.7%), palmitic (16:0, 14.9%-17.9%), stearic (18:0, 4.50%-5.25%), and linoleic acid (18:2, 3.63%-4.6%). Moreover, the most abundant triacylglycerols of papaya seed oil were triolein (OOO), palmitoyl diolein (POO) and stearoyl oleoyl linolein (SOL). In this study, ultrasound-assisted extraction (UAE) significantly (p < 0.05) influenced the triacylglycerol profile of papaya seed oil, but no significant differences were observed in the fatty acid composition of papaya seed oil extracted by different extraction methods (SXE, SE and UAE) and conditions.

Effect of glycerol as co-solvent on yields of bio-oil from rice straw through hydrothermal liquefaction.

PubMed

Cao, Leichang; Zhang, Cheng; Hao, Shilai; Luo, Gang; Zhang, Shicheng; Chen, Jianmin

2016-11-01

This study examined the effect of glycerol used as a co-solvent on yields of bio-oil derived from rice straw through hydrothermal liquefaction (HTL). The reaction was conducted in a high-pressure batch reactor with different volume ratios of glycerol to water. The quality of the derived bio-oil was analyzed in terms of its elemental composition, heating value, water content, ash content, and acid number. Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry were conducted to analyze the chemical composition of the derived bio-oils. The following optimal conditions were obtained: 1:1 vol ratio of glycerol to water with 5wt% of Na2CO3 at 260°C for 1h. Under these conditions, 50.31wt% of bio-oil and 26.65wt% of solid residue were produced. Therefore, glycerol can be used as a co-solvent in HTL of rice straw at moderate temperatures to obtain bio-oil with high yield and quality. Copyright © 2016 Elsevier Ltd. All rights reserved.

Suppression of the SUGAR-DEPENDENT1 triacylglycerol lipase family during seed development enhances oil yield in oilseed rape (Brassica napus L.).

PubMed

Kelly, Amélie A; Shaw, Eve; Powers, Stephen J; Kurup, Smita; Eastmond, Peter J

2013-04-01

Increasing the productivity of oilseed crops is an important challenge for plant breeders and biotechnologists. To date, attempts to increase oil production in seeds via metabolic pathway engineering have focused on boosting synthetic capacity. However, in the tissues of many organisms, it is well established that oil levels are determined by both anabolism and catabolism. Indeed, the oil content of rapeseed (Brassica napus L.) has been reported to decline by approximately 10% in the final stage of development, as the seeds desiccate. Here, we show that RNAi suppression of the SUGAR-DEPENDENT1 triacylglycerol lipase gene family during seed development results in up to an 8% gain in oil yield on either a seed, plant or unit area basis in the greenhouse, with very little adverse impact on seed vigour. Suppression of lipolysis could therefore constitute a new method for enhancing oil yield in oilseed crops. © 2012 The Authors Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.

Bio-oil production from palm fronds by fast pyrolysis process in fluidized bed reactor

NASA Astrophysics Data System (ADS)

Rinaldi, Nino; Simanungkalit, Sabar P.; Kiky Corneliasari, S.

2017-01-01

Fast pyrolysis process of palm fronds has been conducted in the fluidized bed reactor to yield bio-oil product (pyrolysis oil). The process employed sea sand as the heat transfer medium. The objective of this study is to design of the fluidized bed rector, to conduct fast pyrolysis process to product bio-oil from palm fronds, and to characterize the feed and bio-oil product. The fast pyrolysis process was conducted continuously with the feeding rate around 500 g/hr. It was found that the biomass conversion is about 35.5% to yield bio-oil, however this conversion is still minor. It is suggested due to the heating system inside the reactor was not enough to decompose the palm fronds as a feedstock. Moreover, the acids compounds ware mostly observed on the bio-oil product.

Research Progress in Carbon Dioxide Storage and Enhanced Oil Recovery

NASA Astrophysics Data System (ADS)

Wang, Keliang; Wang, Gang; Lu, Chunjing

2018-02-01

With the rapid development of global economy, human beings have become highly dependent upon fossil fuel such as coal and petroleum. Much fossil fuel is consumed in industrial production and human life. As a result, carbon dioxide emissions have been increasing, and the greenhouse effects thereby generated are posing serious threats to environment of the earth. These years, increasing average global temperature, frequent extreme weather events and climatic changes cause material disasters to the world. After scientists’ long-term research, ample evidences have proven that emissions of greenhouse gas like carbon dioxide have brought about tremendous changes to global climate. To really reduce carbon dioxide emissions, governments of different countries and international organizations have invested much money and human resources in performing research related to carbon dioxide emissions. Manual underground carbon dioxide storage and carbon dioxide-enhanced oil recovery are schemes with great potential and prospect for reducing carbon dioxide emissions. Compared with other schemes for reducing carbon dioxide emissions, aforementioned two schemes exhibit high storage capacity and yield considerable economic benefits, so they have become research focuses for reducing carbon dioxide emissions. This paper introduces the research progress in underground carbon dioxide storage and enhanced oil recovery, pointing out the significance and necessity of carbon dioxide-driven enhanced oil recovery.

Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug-flow packed-bed continuous reactor.

PubMed

Kathiele Poppe, Jakeline; Matte, Carla Roberta; Olave de Freitas, Vitória; Fernandez-Lafuente, Roberto; Rodrigues, Rafael C; Záchia Ayub, Marco Antônio

2018-04-30

This work describes the continuous synthesis of ethyl esters via enzymatic catalysis on a packed-bed continuous reactor, using mixtures of immobilized lipases (combi-lipases) of Candida antarctica (CALB), Thermomyces lanuginosus (TLL), and Rhizomucor miehei (RML). The influence of the addition of glass beads to the reactor bed, evaluation of the use of different solvents, and flow rate on reaction conditions were studied. All experiments were conducted using the best combination of lipases according to the fatty acid composition of the waste oil (combi-lipase composition: 40% of TLL, 35% of CALB, and 25% of RML), and soybean oil (combi-lipase composition: 22.5% of TLL, 50% of CALB, and 27.5% of RML). The best general reaction conditions were found to be using tert-butanol as solvent, and the flow rate of 0.08 mL min -1 . The combi-lipase reactors operating at steady state for over 30 days (720 h), kept conversion yields of approximately 50%, with average productivity of 1.94 g ethyl esters g substrate -1 h -1 , regardless of the type of oil in use. This article is protected by copyright. All rights reserved. © 2018 American Institute of Chemical Engineers.

Plastic waste to liquid oil through catalytic pyrolysis using natural and synthetic zeolite catalysts.

PubMed

Miandad, R; Barakat, M A; Rehan, M; Aburiazaiza, A S; Ismail, I M I; Nizami, A S

2017-11-01

This study aims to examine the catalytic pyrolysis of various plastic wastes in the presence of natural and synthetic zeolite catalysts. A small pilot scale reactor was commissioned to carry out the catalytic pyrolysis of polystyrene (PS), polypropylene (PP), polyethylene (PE) and their mixtures in different ratios at 450°C and 75min. PS plastic waste resulted in the highest liquid oil yield of 54% using natural zeolite and 50% using synthetic zeolite catalysts. Mixing of PS with other plastic wastes lowered the liquid oil yield whereas all mixtures of PP and PE resulted in higher liquid oil yield than the individual plastic feedstocks using both catalysts. The GC-MS analysis revealed that the pyrolysis liquid oils from all samples mainly consisted of aromatic hydrocarbons with a few aliphatic hydrocarbon compounds. The types and amounts of different compounds present in liquid oils vary with some common compounds such as styrene, ethylbenzene, benzene, azulene, naphthalene, and toluene. The FT-IR data also confirmed that liquid oil contained mostly aromatic compounds with some alkanes, alkenes and small amounts of phenol group. The produced liquid oils have high heating values (HHV) of 40.2-45MJ/kg, which are similar to conventional diesel. The liquid oil has potential to be used as an alternative source of energy or fuel production. Copyright © 2017 Elsevier Ltd. All rights reserved.

Getty: producing oil from diatomite

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zublin, L.

1981-10-01

Getty Oil Company has developed unconventional oil production techniques which will yield oil from diatomaceous earth. They propose to mine oil-saturated diatomite using open-pit mining methods. Getty's diatomite deposit in the McKittrick field of California is unique because it is cocoa brown and saturated with crude oil. It is classified also as a tightly packed deposit, and oil cannot be extracted by conventional oil field methods.

Method for retorting oil shale

DOEpatents

Shang, Jer-Yu; Lui, A.P.

1985-08-16

The recovery of oil from oil shale is provided in a fluidized bed by using a fluidizing medium of a binary mixture of carbon dioxide and 5 steam. The mixture with a steam concentration in the range of about 20 to 75 volume percent steam provides an increase in oil yield over that achievable by using a fluidizing gas of carbon dioxide or steam alone when the mixture contains higher steam concentrations. The operating parameters for the fluidized bed retorted are essentially the same as those utilized with other gaseous fluidizing mediums with the significant gain being in the oil yield recovered which is attributable solely to the use of the binary mixture of carbon dioxide and steam. 2 figs.

Upgraded bio-oil production via catalytic fast co-pyrolysis of waste cooking oil and tea residual.

PubMed

Wang, Jia; Zhong, Zhaoping; Zhang, Bo; Ding, Kuan; Xue, Zeyu; Deng, Aidong; Ruan, Roger

2017-02-01

Catalytic fast co-pyrolysis (co-CFP) offers a concise and effective process to achieve an upgraded bio-oil production. In this paper, co-CFP experiments of waste cooking oil (WCO) and tea residual (TR) with HZSM-5 zeolites were carried out. The influences of pyrolysis reaction temperature and H/C ratio on pyrolytic products distribution and selectivities of aromatics were performed. Furthermore, the prevailing synergetic effect of target products during co-CFP process was investigated. Experimental results indicated that H/C ratio played a pivotal role in carbon yields of aromatics and olefins, and with H/C ratio increasing, the synergetic coefficient tended to increase, thus led to a dramatic growth of aromatics and olefins yields. Besides, the pyrolysis temperature made a significant contribution to carbon yields, and the yields of aromatics and olefins increased at first and then decreased at the researched temperature region. Note that 600°C was an optimum temperature as the maximum yields of aromatics and olefins could be achieved. Concerning the transportation fuel dependence and security on fossil fuels, co-CFP of WCO and TR provides a novel way to improve the quality and quantity of pyrolysis bio-oil, and thus contributes bioenergy accepted as a cost-competitive and promising alternative energy. Copyright © 2016 Elsevier Ltd. All rights reserved.

Ethanol and High-Value Terpene Co-Production from Lignocellulosic Biomass of Cymbopogon flexuosus and Cymbopogon martinii.

PubMed

Joyce, Blake L; Zheljazkov, Valtcho D; Sykes, Robert; Cantrell, Charles L; Hamilton, Choo; Mann, David G J; Rodriguez, Miguel; Mielenz, Jonathan R; Astatkie, Tess; Stewart, C Neal

2015-01-01

Cymbopogon flexuosus, lemongrass, and C. martinii, palmarosa, are perennial grasses grown to produce essential oils for the fragrance industry. The objectives of this study were (1) to evaluate biomass and oil yields as a function of nitrogen and sulfur fertilization, and (2) to characterize their utility for lignocellulosic ethanol compared to Panicum virgatum (switchgrass). Mean biomass yields were 12.83 Mg lemongrass ha-1 and 15.11 Mg palmarosa ha-1 during the second harvest year resulting in theoretical biofuel yields of 2541 and 2569 L ethanol ha-1 respectively compared to reported 1749-3691 L ethanol ha-1 for switchgrass. Pretreated lemongrass yielded 198 mL ethanol (g biomass)-1 and pretreated palmarosa yielded 170 mL ethanol (g biomass)-1. Additionally, lemongrass yielded 85.7 kg essential oil ha-1 and palmarosa yielded 67.0 kg ha-1 with an estimated value of USD $857 and $1005 ha-1. These data suggest that dual-use crops such as lemongrass and palmarosa may increase the economic viability of lignocellulosic biofuels.

Ethanol and High-Value Terpene Co-Production from Lignocellulosic Biomass of Cymbopogon flexuosus and Cymbopogon martinii

DOE PAGES

Joyce, Blake L.; Zheljazkov, Valtcho D.; Sykes, Robert; ...

2015-10-05

Cymbopogon flexuosus, lemongrass, and C. martinii, palmarosa, are perennial grasses grown to produce essential oils for the fragrance industry. The objectives of this study were (1) to evaluate biomass and oil yields as a function of nitrogen and sulfur fertilization, and (2) to characterize their utility for lignocellulosic ethanol compared to Panicum virgatum (switchgrass). Mean biomass yields were 12.83 Mg lemongrass ha -1 and 15.11 Mg palmarosa ha -1 during the second harvest year resulting in theoretical biofuel yields of 2541 and 2569 L ethanol ha -1 respectively compared to reported 1749–3691 L ethanol ha -1 for switchgrass. Pretreated lemongrassmore » yielded 198 mL ethanol (g biomass) -1 and pretreated palmarosa yielded 170 mL ethanol (g biomass) -1. Additionally, lemongrass yielded 85.7 kg essential oil ha-1 and palmarosa yielded 67.0 kg ha -1 with an estimated value of USD $857 and $1005 ha -1. These data suggest that dual-use crops such as lemongrass and palmarosa may increase the economic viability of lignocellulosic biofuels.« less

Ethanol and High-Value Terpene Co-Production from Lignocellulosic Biomass of Cymbopogon flexuosus and Cymbopogon martinii

PubMed Central

Joyce, Blake L.; Zheljazkov, Valtcho D.; Sykes, Robert; Cantrell, Charles L.; Hamilton, Choo; Mann, David G. J.; Rodriguez, Miguel; Mielenz, Jonathan R.; Astatkie, Tess; Stewart, C. Neal

2015-01-01

Cymbopogon flexuosus, lemongrass, and C. martinii, palmarosa, are perennial grasses grown to produce essential oils for the fragrance industry. The objectives of this study were (1) to evaluate biomass and oil yields as a function of nitrogen and sulfur fertilization, and (2) to characterize their utility for lignocellulosic ethanol compared to Panicum virgatum (switchgrass). Mean biomass yields were 12.83 Mg lemongrass ha-1 and 15.11 Mg palmarosa ha-1 during the second harvest year resulting in theoretical biofuel yields of 2541 and 2569 L ethanol ha-1 respectively compared to reported 1749–3691 L ethanol ha-1 for switchgrass. Pretreated lemongrass yielded 198 mL ethanol (g biomass)-1 and pretreated palmarosa yielded 170 mL ethanol (g biomass)-1. Additionally, lemongrass yielded 85.7 kg essential oil ha-1 and palmarosa yielded 67.0 kg ha-1 with an estimated value of USD $857 and $1005 ha-1. These data suggest that dual-use crops such as lemongrass and palmarosa may increase the economic viability of lignocellulosic biofuels. PMID:26437026

Oil production by entrained pyrolysis of biomass and processing of oil and char

DOEpatents

Knight, James A.; Gorton, Charles W.

1990-01-02

Entrained pyrolysis of lignocellulosic material proceeds from a controlled pyrolysis-initiating temperature to completion of an oxygen free environment at atmospheric pressure and controlled residence time to provide a high yield recovery of pyrolysis oil together with char and non-condensable, combustible gases. The residence time is a function of gas flow rate and the initiating temperature is likewise a function of the gas flow rate, varying therewith. A controlled initiating temperature range of about 400.degree. C. to 550.degree. C. with corresponding gas flow rates to maximize oil yield is disclosed.

Extraction of Oil from Flaxseed (Linum usitatissimum L.) Using Enzyme-Assisted Three-Phase Partitioning.

PubMed

Tan, Zhi-Jian; Yang, Zi-Zhen; Yi, Yong-Jian; Wang, Hong-Ying; Zhou, Wan-Lai; Li, Fen-Fang; Wang, Chao-Yun

2016-08-01

In this study, enzyme-assisted three-phase partitioning (EATPP) was used to extract oil from flaxseed. The whole procedure is composed of two parts: the enzymolysis procedure in which the flaxseed was hydrolyzed using an enzyme solution (the influencing parameters such as the type and concentration of enzyme, temperature, and pH were optimized) and three-phase partitioning (TPP), which was conducted by adding salt and t-butanol to the crude flaxseed slurry, resulting in the extraction of flaxseed oil into alcohol-rich upper phase. The concentration of t-butanol, concentration of salt, and the temperature were optimized to maximize the extraction yield. Under optimized conditions of a 49.29 % t-butanol concentration, 30.43 % ammonium sulfate concentration, and 35 °C extraction temperature, a maximum extraction yield of 71.68 % was obtained. This simple and effective EATPP can be used to achieve high extraction yields and oil quality, and thus, it is potential for large-scale oil production.

[Comparison on extraction of volatile oils from Lithospermum erythrorhizon by different methods].

PubMed

Yang, Ri-fu; Huang, Ping-ping; Qiu, Tai-qiu; Fan, Xiao-dan

2011-02-01

To extract the volatile oils from Lithospermum erythrorhizon via ultrasound-enhanced sub-critical water extraction (USWE) and compare with ultrasound-enhanced solvent extraction (USE) and steam distillation extraction (SD). The extraction yield of the volatile oils, the containing components of extract, the effect of scanvenging activities on free radical DPPH and reducing activities as well as the inhibitory on escherichia coli and staphylococcus aureus were investigated. The extraction yield of volatile oils by USWE, USE and SD were 2.39%, 1.93% and 0.62%, respectively, the extracts by three methods all contained six major components, but the extracts by SD and USE contained more impurities. The inhibitory effect on escherichia coli and staphylococcus aureus of the extract by SD and its reducing action were the best,but those by USWE were the worst. the extraction yield of volatile oils by USWE is the highest, and it contains less impurities based on the worst in reducing power and inhibitory effects.

A comparison of essential oils obtained from lavandin via different extraction processes: Ultrasound, microwave, turbohydrodistillation, steam and hydrodistillation.

PubMed

Périno-Issartier, Sandrine; Ginies, Christian; Cravotto, Giancarlo; Chemat, Farid

2013-08-30

A total of eight extraction techniques ranging from conventional methods (hydrodistillation (HD), steam distillation (SD), turbohydrodistillation (THD)), through innovative techniques (ultrasound assisted extraction (US-SD) and finishing with microwave assisted extraction techniques such as In situ microwave-generated hydrodistillation (ISMH), microwave steam distillation (MSD), microwave hydrodiffusion and gravity (MHG), and microwave steam diffusion (MSDf)) were used to extract essential oil from lavandin flowers and their results were compared. Extraction time, yield, essential oil composition and sensorial analysis were considered as the principal terms of comparison. The essential oils extracted using the more innovative processes were quantitatively (yield) and qualitatively (aromatic profile) similar to those obtained from the conventional techniques. The method which gave the best results was the microwave hydrodiffusion and gravity (MHG) method which gave reduced extraction time (30min against 220min for SD) and gave no differences in essential oil yield and sensorial perception. Copyright © 2013 Elsevier B.V. All rights reserved.

Variability and performance evaluation of introgressed Nigerian dura x Deli dura oil palm progenies.

PubMed

Noh, A; Rafii, M Y; Mohd Din, A; Kushairi, A; Norziha, A; Rajanaidu, N; Latif, M A; Malek, M A

2014-04-03

Twelve introgressed oil palm (Elaeis guineensis) progenies of Nigerian dura x Deli dura were evaluated for bunch yield, yield attributes, bunch quality components and vegetative characters at the Malaysian Palm Oil Board Research Station, in Keratong, Pahang, Malaysia. Analysis of variance revealed significant to highly significant genotypic differences, indicating sufficient genetic variability among the progenies for bunch yield and its attributes, vegetative characters and bunch quality components, except fruit to bunch ratio. Fresh fruit bunch yield ranged from 167 kg·palm(-1)·year(-1) in PK1330 to 212 kg·palm(-1)·year(-1) in PK1351, with a mean yield of 192 kg·palm(-1)·year(-1). Among the progeny, PK1313 had the highest oil to bunch ratio (19.36%), due to its high mesocarp to fruit ratio, fruit to bunch ratio and low shell to fruit ratio. Among the progenies, PK1313 produced the highest oil yield of 31.4 kg·palm(-1)·year(-1), due to a high mesocarp to fruit ratio (61.2%) and a low shell to fruit ratio (30.7%), coupled with high fruit to bunch ratio (65.6%). PK1330 was found promising for selection, as it had desirable vegetative characters, including smaller petiole cross section (27.15 cm2), short rachis length (4.83 m), short palm height (1.85 m), and the lowest leaf number (164.6), as these vegetative characters are prerequisites for selecting palms for high density planting and high yield per hectare. The genetic variability among the progenies was found to be high, indicating ample scope for further breeding, followed by selection.

Multifunctional two-stage riser fluid catalytic cracking process.

PubMed

Zhang, Jinhong; Shan, Honghong; Chen, Xiaobo; Li, Chunyi; Yang, Chaohe

This paper described the discovering process of some shortcomings of the conventional fluid catalytic cracking (FCC) process and the proposed two-stage riser (TSR) FCC process for decreasing dry gas and coke yields and increasing light oil yield, which has been successfully applied in 12 industrial units. Furthermore, the multifunctional two-stage riser (MFT) FCC process proposed on the basis of the TSR FCC process was described, which were carried out by the optimization of reaction conditions for fresh feedstock and cycle oil catalytic cracking, respectively, by the coupling of cycle oil cracking and light FCC naphtha upgrading processes in the second-stage riser, and the specially designed reactor for further reducing the olefin content of gasoline. The pilot test showed that it can further improve the product quality, increase the diesel yield, and enhance the conversion of heavy oil.

Development of a highly efficient oil degumming process using a novel phosphatidylinositol-specific phospholipase C enzyme.

PubMed

Cerminati, Sebastián; Eberhardt, Florencia; Elena, Claudia E; Peirú, Salvador; Castelli, María E; Menzella, Hugo G

2017-06-01

Enzymatic degumming using phospholipase C (PLC) enzymes may be used in environmentally friendly processes with improved oil recovery yields. In this work, phosphatidylinositol-specific phospholipase C (PIPLC) candidates obtained from an in silico analysis were evaluated for oil degumming. A PIPLC from Lysinibacillus sphaericus was shown to efficiently remove phosphatidylinositol from crude oil, and when combined with a second phosphatidylcholine and phosphatidylethanolamine-specific phospholipase C, the three major phospholipids were completely hydrolyzed, providing an extra yield of oil greater than 2.1%, compared to standard methods. A remarkably efficient fed-batch Escherichia coli fermentation process producing ∼14 g/L of the recombinant PIPLC enzyme was developed, which may facilitate the adoption of this cost-effective oil-refining process.

Improving oxidative stability of echium oil emulsions fabricated by Microfluidics: Effect of ionic gelation and phenolic compounds.

PubMed

Comunian, Talita A; Ravanfar, Raheleh; de Castro, Inar Alves; Dando, Robin; Favaro-Trindade, Carmen S; Abbaspourrad, Alireza

2017-10-15

Echium oil is rich in omega-3 fatty acids, which are important because of their benefits to human health; it is, however, unstable. The objective of this work was the coencapsulation of echium oil and quercetin or sinapic acid by microfluidic and ionic gelation techniques. The treatments were analyzed utilizing optical and scanning electron microscopy, encapsulation yield, particle size, thermogravimetry, Fourier transform infrared spectroscopy, stability under stress conditions, and oil oxidative/phenolic compound stability for 30days at 40°C. High encapsulation yield values were obtained (91-97% and 77-90% for the phenolic compounds and oil) and the encapsulated oil was almost seven times more stable than the non-encapsulated oil (0.34 vs 2.42mgMDA/kg oil for encapsulated and non-encapsulated oil, respectively). Encapsulation was shown to promote oxidative stability, allowing new vehicles for the application of these compounds in food without the use of solvents and high temperature. Copyright © 2017 Elsevier Ltd. All rights reserved.

Salinity impact on yield, water use, mineral and essential oil content of fennel (Foeniculum vulgare Mill.)

USDA-ARS?s Scientific Manuscript database

The experimental study was carried out to determine the effects of salinity on water consumption, plant height, fresh and seed yields, biomass production, ion accumulation and essential oil content of fennel (Foeniculum vulgare Mill.) under greenhouse conditions. The experiment was conducted with a ...

Experimental investigation of the role of rock fabric in gas generation and expulsion during thermal maturation: Anhydrous closed-system pyrolysis of a bitumen-rich Eagle Ford Shale

USGS Publications Warehouse

Shao, Deyong; Ellis, Geoffrey S.; Li, Yanfang; Zhang, Tongwei

2018-01-01

Gold-tube pyrolysis experiments were conducted on miniature core plugs and powdered rock from a bitumen-rich sample of Eagle Ford Shale to investigate the role of rock fabric in gas generation and expulsion during thermal maturation. The samples were isothermally heated at 130, 300, 310, 333, 367, 400, and 425 °C for 72 h under a confining pressure of 68.0 MPa, corresponding to six levels of induced thermal maturity: pre-oil generation (130 °C/72 h), incipient oil/bitumen generation (300 and 310 °C/72 h), early oil generation (333 °C/72 h), peak oil generation (367 °C/72 h), early oil cracking (400 °C/72 h), and late oil cracking (425 °C/72 h). Experimental results show that gas retention coupled with compositional fractionation occurs in the core plug experiments and varies as a function of thermal maturity. During the incipient oil/bitumen generation stage, yields of methane through pentane (C1–C5) from core plugs are significantly lower than those from rock powder, and gases from core plugs are enriched in methane. However, the differences in C1–C5 gas yield and composition decrease throughout the oil generation stage, and by the oil cracking stage no obvious compositional difference in C1–C5 gases exists. The decrease in the effect of rock fabric on gas yield and composition with increasing maturity is the result of an increase in gas expulsion efficiency. Pyrolysis of rock powder yields 4–16 times more CO2 compared to miniature core plugs, with δ13CCO2 values ranging from −2.9‰ to −0.6‰, likely due to carbonate decomposition accelerated by reactions with organic acids. Furthermore, lower yields of gaseous alkenes and H2 from core plug experiments sugge

Techno-economic and resource analysis of hydroprocessed renewable jet fuel

DOE PAGES

Tao, Ling; Milbrandt, Anelia; Zhang, Yanan; ...

2017-11-09

Biomass-derived jet fuel is an alternative jet fuel (AJF) showing promise of reducing the dependence on fossil fuel and greenhouse gas emissions. Hydroprocessed esters and fatty acids (HEFA) concept is also known as one of the pathways for producing bio jet fuel. HEFA fuel was approved by the American Society for Testing and Materials in 2011, and can be blended up to 50% with conventional jet fuel. Since then, several HEFA economic and life-cycle assessments have been published in literature. However, there have been limited analyses on feedstock availability, composition, and their impact on hydrocarbon yield (particularly jet blendstock yield)more » and overall process economics. Our study examines over 20 oil feedstocks, their geographic distribution and production levels, oil yield, prices, and chemical composition. The results of our compositional analysis, thus, indicate that most oils contain mainly C16 and C18 fatty acids except pennycress, yellow grease, and mustard, which contain higher values and thus would require hydrocracking to improve jet fuel production. Coconut oil has a large content of shorter carbon fatty acids, making it a good feedstock candidate for renewable gasoline instead of jet substitutes' production. Techno-economic analysis (TEA) was performed for five selected oil feedstocks - camelina, pennycress, jatropha, castor bean, and yellow grease - using the HEFA process concept. The resource analysis indicates that oil crops currently grown in the United States (namely soybean) have relatively low oil yield when compared to oil crops grown in other parts of the world, such as palm, coconut, and jatropha. Also, non-terrestrial oil sources, such as animal fats and greases, have relatively lower prices than terrestrial oil crops. The minimum jet fuel selling price for these five resources ranges between $3.8 and $11.0 per gallon. The results of our TEA and resource studies indicate the key cost drivers for a biorefinery converting oil to jet hydrocarbons are as follows: oil price, conversion plant capacity, fatty acid profile, addition of hydrocracker, and type of hydroprocessing catalysts.« less

Techno-economic and resource analysis of hydroprocessed renewable jet fuel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tao, Ling; Milbrandt, Anelia; Zhang, Yanan

Biomass-derived jet fuel is an alternative jet fuel (AJF) showing promise of reducing the dependence on fossil fuel and greenhouse gas emissions. Hydroprocessed esters and fatty acids (HEFA) concept is also known as one of the pathways for producing bio jet fuel. HEFA fuel was approved by the American Society for Testing and Materials in 2011, and can be blended up to 50% with conventional jet fuel. Since then, several HEFA economic and life-cycle assessments have been published in literature. However, there have been limited analyses on feedstock availability, composition, and their impact on hydrocarbon yield (particularly jet blendstock yield)more » and overall process economics. Our study examines over 20 oil feedstocks, their geographic distribution and production levels, oil yield, prices, and chemical composition. The results of our compositional analysis, thus, indicate that most oils contain mainly C16 and C18 fatty acids except pennycress, yellow grease, and mustard, which contain higher values and thus would require hydrocracking to improve jet fuel production. Coconut oil has a large content of shorter carbon fatty acids, making it a good feedstock candidate for renewable gasoline instead of jet substitutes' production. Techno-economic analysis (TEA) was performed for five selected oil feedstocks - camelina, pennycress, jatropha, castor bean, and yellow grease - using the HEFA process concept. The resource analysis indicates that oil crops currently grown in the United States (namely soybean) have relatively low oil yield when compared to oil crops grown in other parts of the world, such as palm, coconut, and jatropha. Also, non-terrestrial oil sources, such as animal fats and greases, have relatively lower prices than terrestrial oil crops. The minimum jet fuel selling price for these five resources ranges between $3.8 and $11.0 per gallon. The results of our TEA and resource studies indicate the key cost drivers for a biorefinery converting oil to jet hydrocarbons are as follows: oil price, conversion plant capacity, fatty acid profile, addition of hydrocracker, and type of hydroprocessing catalysts.« less

Cessation of oil exposure in harlequin ducks after the Exxon Valdez oil spill: Cytochrome P4501A biomarker evidence

USGS Publications Warehouse

Esler, Daniel N.; Ballachey, Brenda E.; Bowen, Lizabeth; Miles, A. Keith; Dickson, Rian D.; Henderson, John D.

2017-01-01

The authors quantified hepatic hydrocarbon-inducible cytochrome P4501A (CYP1A) expression, as ethoxyresorufin-O-deethylase (EROD) activity, in wintering harlequin ducks (Histrionicus histrionicus) captured in Prince William Sound, Alaska (USA), during 2011, 2013, and 2014 (22–25 yr following the 1989 Exxon Valdez oil spill). Average EROD activity was compared between birds from areas oiled by the spill and those from nearby unoiled areas. The present study replicated studies conducted from 1998 to 2009 demonstrating that harlequin ducks using areas oiled in 1989 had elevated EROD activity, indicative of oil exposure, up to 2 decades post spill. In the present study, it was found that average EROD activity during March 2011 was significantly higher in wintering harlequin ducks captured in oiled areas relative to unoiled areas, which the authors interpret to indicate that harlequin ducks continued to be exposed to residual Exxon Valdez oil up to 22 yr after the original spill. However, the 2011 results also indicated reductions in exposure relative to previous years. Average EROD activity in birds from oiled areas was approximately 2 times that in birds from unoiled areas in 2011, compared with observations from 2005 to 2009, in which EROD activity was 3 to 5 times higher in oiled areas. It was also found that average EROD activity during March 2013 and March 2014 was not elevated in wintering harlequin ducks from oiled areas. The authors interpret these findings to indicate that exposure of harlequin ducks to residual Exxon Valdez oil abated within 24 yr after the original spill. The present study finalizes a timeline of exposure, extending over 2 decades, for a bird species thought to be particularly vulnerable to oil contamination in marine environments

Influence of Phenological Stages on Yield and Quality of Oregano (Origanum vulgare L.) Under the Agroclimatic Condition of Doon Valley (Uttarakhand)

PubMed Central

Chauhan, N. K.; Singh, S.; Haider, S. Z.; Lohani, H.

2013-01-01

A field experiment was conducted under the agroclimatic conditions of Doon valley, in order to determine the effects of phenological stages on herbage yield and quality of oil in oregano (Origanum vulgare L.). Plants were harvested in five phenological stages, i.e. early vegetative, late vegetative, flower initiation, full bloom, and fruit set stages. Results showed the significant effects of phenological stages on herbage, yield, and quality of oregano. Harvesting at full bloom stage showed better results in terms of herbage and oil yield. The quality of essential oil was evaluated using GC and GC/MS. Thymol content was rich in all the stages (46.90-62.26%) followed by γ-terpinene (1.11-11.75%) and p-cymene (3.11-5.32%). PMID:24302806

Manufacturing vegetable oil based biodiesel: An engineering management perspective

USDA-ARS?s Scientific Manuscript database

According to the USDA, 6.45 million tons of cottonseed was produced in 2007. Each ton will yield approximately 44 to 46 gallons unrefined oil. Cottonseed oil bio-diesel could have the potential to create a more competitive oil market for oil mills. The proposed cost model is based on an existing cot...

Diversity, distribution and hydrocarbon biodegradation capabilities of microbial communities in oil-contaminated cyanobacterial mats from a constructed wetland.

PubMed

Abed, Raeid M M; Al-Kharusi, Samiha; Prigent, Stephane; Headley, Tom

2014-01-01

Various types of cyanobacterial mats were predominant in a wetland, constructed for the remediation of oil-polluted residual waters from an oil field in the desert of the south-eastern Arabian Peninsula, although such mats were rarely found in other wetland systems. There is scarce information on the bacterial diversity, spatial distribution and oil-biodegradation capabilities of freshwater wetland oil-polluted mats. Microbial community analysis by Automated Ribosomal Spacer Analysis (ARISA) showed that the different mats hosted distinct microbial communities. Average numbers of operational taxonomic units (OTUsARISA) were relatively lower in the mats with higher oil levels and the number of shared OTUsARISA between the mats was <60% in most cases. Multivariate analyses of fingerprinting profiles indicated that the bacterial communities in the wetland mats were influenced by oil and ammonia levels, but to a lesser extent by plant density. In addition to oil and ammonia, redundancy analysis (RDA) showed also a significant contribution of temperature, dissolved oxygen and sulfate concentration to the variations of the mats' microbial communities. Pyrosequencing yielded 282,706 reads with >90% of the sequences affiliated to Proteobacteria (41% of total sequences), Cyanobacteria (31%), Bacteriodetes (11.5%), Planctomycetes (7%) and Chloroflexi (3%). Known autotrophic (e.g. Rivularia) and heterotrophic (e.g. Azospira) nitrogen-fixing bacteria as well as purple sulfur and non-sulfur bacteria were frequently encountered in all mats. On the other hand, sequences of known sulfate-reducing bacteria (SRBs) were rarely found, indicating that SRBs in the wetland mats probably belong to yet-undescribed novel species. The wetland mats were able to degrade 53-100% of C12-C30 alkanes after 6 weeks of incubation under aerobic conditions. We conclude that oil and ammonia concentrations are the major key players in determining the spatial distribution of the wetland mats' microbial communities and that these mats contribute directly to the removal of hydrocarbons from oil field wastewaters.

Activity antifungal of the essential oils; aqueous and ethanol extracts from Citrus aurantium L.

PubMed

Metoui, N; Gargouri, S; Amri, I; Fezzani, T; Jamoussi, B; Hamrouni, L

2015-01-01

Our study is about the essential oil of Citrus aurantium L. in Tunisia and its plant extract. The yield of this essential oil is 0, 56% but the yield of the extract of plant was 17.1% for the aqueous extract ant 18.3% for the ethanolic extract. The analysis of chemical composition by using GC and GC/MS showed the essential oil of C. aurantium L. species to be rich in monoterpenes such as α-terpineol, lianolyl acetate, linalool and limonene. The antifungal activity of this oil showed us an inhibition of the germination of mushrooms, in the same way we could note that the biologic activities are generally assigned to the chemotypes high content in oxygenated monoterpene.

Phase separation of bio-oil produced by co-pyrolysis of corn cobs and polypropylene

NASA Astrophysics Data System (ADS)

Supramono, D.; Julianto; Haqqyana; Setiadi, H.; Nasikin, M.

2017-11-01

In co-pyrolysis of biomass-plastics, bio-oil produced contains both oxygenated and non-oxygenated compounds. High oxygen composition is responsible for instability and low heating value of bio-oil and high acid content for corrosiveness. Aims of the present work are to evaluate possibilities of achieving phase separation between oxygenated and non-oxygenated compounds in bio-oil using a proposed stirred tank reactor and to achieve synergistic effects on bio-oil yield and non-oxygenated compound layer yield. Separation of bio-oil into two layers, i.e. that containing oxygenated compounds (polar phase) and non-oxygenated compounds (non-polar phase) is important to obtain pure non-polar phase ready for the next processing of hydrogenation and used directly as bio-fuel. There has been no research work on co-pyrolysis of biomass-plastic considering possibility of phase separation of bio-oil. The present work is proposing a stirred tank reactor for co-pyrolysis with nitrogen injection, which is capable of tailoring co-pyrolysis conditions leading to low viscosity and viscosity asymmetry, which induce phase separation between polar phase and non-polar phase. The proposed reactor is capable of generating synergistic effect on bio-oil and non-polar yields as the composition of PP in feed is more than 25% weight in which non-polar layers contain only alkanes, alkenes, cycloalkanes and cycloalkenes.

Ozone dose-response relationships for spring oilseed rape and broccoli

NASA Astrophysics Data System (ADS)

De Bock, Maarten; Op de Beeck, Maarten; De Temmerman, Ludwig; Guisez, Yves; Ceulemans, Reinhart; Vandermeiren, Karine

2011-03-01

Tropospheric ozone is an important air pollutant with known detrimental effects for several crops. Ozone effects on seed yield, oil percentage, oil yield and 1000 seed weight were examined for spring oilseed rape ( Brassica napus cv. Ability). For broccoli ( Brassica oleracea L. cv. Italica cv. Monaco) the effects on fresh marketable weight and total dry weight were studied. Current ozone levels were compared with an increase of 20 and 40 ppb during 8 h per day, over the entire growing season. Oilseed rape seed yield was negatively correlated with ozone dose indices calculated from emergence until harvest. This resulted in an R2 of 0.24 and 0.26 ( p < 0.001) for the accumulated hourly O 3 exposure over a threshold of 40 ppb (AOT40) and the phytotoxic ozone dose above a threshold of 6 nmol m -2 s -1 (POD 6) respectively. Estimated critical levels, above which 5% yield reduction is expected, were 3.7 ppm h and 4.4 mmol m -2 respectively. Our results also confirm that a threshold value of 6 nmol s -1 m -2 projected leaf area, as recommended for agricultural crops (UNECE, Mills, 2004), can indeed be applied for spring oilseed rape. The reduction of oilseed rape yield showed the highest correlation with the ozone uptake during the vegetative growth stage: when only the first 47 days after emergence were used to calculate POD 6, R2 values increased up to 0.476 or even 0.545 when the first 23 days were excluded. The highest ozone treatments, corresponding to the future ambient level by 2100 (IPCC, Meehl et al., 2007), led to a reduction of approximately 30% in oilseed rape seed yield in comparison to the current ozone concentrations. Oil percentage was also significantly reduced in response to ozone ( p < 0.001). As a consequence oil yield was even more severely affected by elevated ozone exposure compared to seed yield: critical levels for oil yield dropped to 3.2 ppm h and 3.9 mmol m -2. For broccoli the applied ozone doses had no effect on yield.

Starch-Soybean Oil Composites with High Oil: Starch Ratios Prepared by Steam Jet Cooking

USDA-ARS?s Scientific Manuscript database

Aqueous mixtures of soybean oil and starch were jet cooked at oil:starch ratios ranging from 0.5:1 to 4:1 to yield dispersions of micron-sized oil droplets that were coated with a thin layer of starch at the oil-water interface. The jet cooked dispersions were then centrifuged at 2060 and 10,800 x ...

Optimising the operational parameters of a spherical steriliser for the treatment of oil palm fresh fruit bunch

NASA Astrophysics Data System (ADS)

Kumaradevan, D.; Chuah, K. H.; Moey, L. K.; Mohan, V.; Wan, W. T.

2015-09-01

The extraction of crude palm oil (CPO) begins with the sterilization of oil palm fresh fruit bunch (FFB) in a pressurized, saturated-steam chamber. Sterilization loosens the palm fruits from the stalks and deactivates the free fatty acid (FFA)-producing enzymes. Operational parameters affecting the quality and yield of CPO from an industrial spherical sterilizer are studied at a palm oil mill. The factors are the ripeness of FFB, the number of days before treatment of FFB, and the number of pressure peaks applied in the sterilization process. The results indicate that the degree of ripeness of FFB is the most important parameter affecting the quality and yield of CPO. Ripeness is graded based on the fruits’ colour and the presence of loose fruits. Over ripe FFB that goes for the sterilization process has higher FFA content in CPO and more oil loss to the condensate chamber. The spontaneous reaction on FFB due to accumulation at the loading ramp also gives rise to higher FFA content. Oil loss to condensate chamber is reduced using a two-peak sterilization technique for over ripe FFB; the peak refers to the pressure level of stream after a flushing and refilling cycle. Overall, the generated solution improves the quality and yield of the palm oil mill.

Influence of a glass wool hot vapour filter on yields and properties of bio-oil derived from rapid pyrolysis of paddy residues.

PubMed

Pattiya, Adisak; Suttibak, Suntorn

2012-07-01

This article reports experimental results of rapid or fast pyrolysis of rice straw (RS) and rice husk (RH) in a fluidised-bed reactor unit incorporated with a hot vapour filter. The objective of this research was to investigate the effects of pyrolysis temperatures and the use of glass wool hot vapour filtration on pyrolysis products. The results showed that the optimum pyrolysis temperatures for RS and RH were 405 and 452 °C, which gave maximum bio-oil yields of 54.1 and 57.1 wt.% on dry biomass basis, respectively. The use of the hot filter led to a reduction of 4-7 wt.% bio-oil yield. Nevertheless, the glass wool hot filtered bio-oils appeared to have better quality in terms of initial viscosity, solids content and ash content than the non-filtered ones. Copyright © 2012 Elsevier Ltd. All rights reserved.

The role of ants, birds and bats for ecosystem functions and yield in oil palm plantations.

PubMed

Denmead, Lisa H; Darras, Kevin; Clough, Yann; Diaz, Patrick; Grass, Ingo; Hoffmann, Munir P; Nurdiansyah, Fuad; Fardiansah, Rico; Tscharntke, Teja

2017-07-01

One of the world's most important and rapidly expanding crops, oil palm, is associated with low levels of biodiversity. Changes in predator communities might alter ecosystem services and subsequently sustainable management but these links have received little attention to date. Here, for the first time, we manipulated ant and flying vertebrate (birds and bats) access to oil palms in six smallholder plantations in Sumatra (Indonesia) and measured effects on arthropod communities, related ecosystem functions (herbivory, predation, decomposition and pollination) and crop yield. Arthropod predators increased in response to reductions in ant and bird access, but the overall effect of experimental manipulations on ecosystem functions was minimal. Similarly, effects on yield were not significant. We conclude that ecosystem functions and productivity in oil palm are, under current levels of low pest pressure and large pollinator populations, robust to large reductions of major predators. © 2017 by the Ecological Society of America.

Co-production of biochar, bio-oil and syngas from halophyte grass (Achnatherum splendens L.) under three different pyrolysis temperatures.

PubMed

Irfan, Muhammad; Chen, Qun; Yue, Yan; Pang, Renzhong; Lin, Qimei; Zhao, Xiaorong; Chen, Hao

2016-07-01

In the present study, pyrolysis of Achnatherum splendens L. was performed under three different pyrolysis temperature (300, 500, and 700°C) to investigate the characteristics of biochar, bio-oil, and syngas. Biochar yield decreased from 48% to 24%, whereas syngas yield increased from 34% to 54% when pyrolysis temperature was increased from 300 to 700°C. Maximum bio-oil yield (27%) was obtained at 500°C. The biochar were characterized for elemental composition, surface, and adsorption properties. The results showed that obtained biochar could be used as a potential soil amendment. The bio-oil and syngas co-products will be evaluated in the future as bioenergy sources. Overall, our results suggests that A. splendens L. could be utilized as a potential feedstock for biochar and bioenergy production through pyrolytic route. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

The slow and fast pyrolysis of cherry seed.

PubMed

Duman, Gozde; Okutucu, Cagdas; Ucar, Suat; Stahl, Ralph; Yanik, Jale

2011-01-01

The slow and fast pyrolysis of cherry seeds (CWS) and cherry seeds shells (CSS) was studied in fixed-bed and fluidized bed reactors at different pyrolysis temperatures. The effects of reactor type and temperature on the yields and composition of products were investigated. In the case of fast pyrolysis, the maximum bio-oil yield was found to be about 44 wt% at pyrolysis temperature of 500 °C for both CWS and CSS, whereas the bio yields were of 21 and 15 wt% obtained at 500 °C from slow pyrolysis of CWS and CSS, respectively. Both temperature and reactor type affected the composition of bio-oils. The results showed that bio-oils obtained from slow pyrolysis of CWS and CSS can be used as a fuel for combustion systems in industry and the bio-oil produced from fast pyrolysis can be evaluated as a chemical feedstock. Copyright © 2010 Elsevier Ltd. All rights reserved.

Ultrasonic-assisted extraction of essential oil from Botryophora geniculate using different extracting solvents

NASA Astrophysics Data System (ADS)

Habibullah, Wilfred, Cecilia Devi

2016-11-01

This study compares the performance of ionic liquids to substitute conventional solvents (hexane, dichloromethane and methanol) to extract essential oil from Botryophora geniculate plant. Two different Ionic liquids ([C3MIM][Ac], [C4MIM][Ac]) with co-solvent diethyl ether were used in the ultrasonic-assisted extraction. The effect of various experimental conditions such as time, temperature and solvent were studied. Gas chromatography-mass spectroscopy (GC-MS) was used to analyze essential oils. The results showed that in ultrasonic-assisted extraction using ionic liquids as a solvent gave highest yield (9.5%) in 30 min at temperature 70°C. When using ultrasonic bath with hexane, dichloromethane and methanol, yields was (3.34%), (3.6%) and (3.81%) at 90 min, respectively were obtained. The ultrasonic-assisted extraction under optimal extraction conditions (time 30 min, temperature of 70°C) gave the best yield for the essential oil extraction.

ESolvent-free, enzyme-catalyzed biodiesel production from mango, neem, and shea oils via response surface methodology.

PubMed

Nde, Divine Bup; Astete, Carlos; Boldor, Dorin

2015-12-01

Mango, neem and shea kernels produce non-conventional oils whose potentials are not fully exploited. To give an added value to these oils, they were transesterified into biodiesel in a solvent-free system using immobilized enzyme lipozyme from Mucor miehei. The Doehlert experimental design was used to evaluate the methyl ester (ME) yields as influenced by enzyme concentration-EC, temperature-T, added water content-AWC, and reaction time-RT. Biodiesel yields were quantified by (1)H NMR spectroscopy and subsequently modeled by a second order polynomial equation with interactions. Lipozyme enzymes were more tolerant to high temperatures in neem and shea oils reaction media compared to that of mango oil. The optimum reaction conditions EC, T, AWC, and RT assuring near complete conversion were as follows: mango oil 7.25 %, 36.6 °C, 10.9 %, 36.4 h; neem oil EC = 7.19 %, T = 45.7 °C, AWC = 8.43 %, RT = 25.08 h; and shea oil EC = 4.43 %, T = 45.65 °C, AWC = 6.21 % and RT = 25.08 h. Validation experiments of these optimum conditions gave ME yields of 98.1 ± 1.0, 98.5 ± 1.6 and 99.3 ± 0.4 % for mango, neem and shea oils, respectively, which all met ASTM biodiesel standards.

Cosuppression of limonene-3-hydroxylase in peppermint promotes accumulation of limonene in the essential oil.

PubMed

Mahmoud, Soheil S; Williams, Matthew; Croteau, Rodney

2004-03-01

cDNA clones encoding limonene synthase and limonene-3-hydroxylase, both driven by the CaMV 35S promoter, were independently transformed into peppermint (Menthaxpiperita) to alter the production and disposition of (-)-limonene, the first committed intermediate of essential oil biosynthesis in this species. Although both genes were constitutively expressed in leaves of transformed plants, the corresponding enzyme activities were not significantly increased in the glandular trichome sites of essential oil biosynthesis; thus, there was no effect on oil yield or composition in the regenerated plants. Cosuppression of the hydroxylase gene, however, resulted in the accumulation of limonene (up to 80% of the essential oil compared to about 2% of the oil in wild type plants), without influence on oil yield. These results indicate that limonene does not impose negative feedback on the synthase, or apparently influence other enzymes of monoterpene biosynthesis in peppermint, and suggests that pathway engineering can be employed to significantly alter essential oil composition without adverse metabolic consequences.

A comparative study of bio-oils from pyrolysis of microalgae and oil seed waste in a fluidized bed.

PubMed

Kim, Sung Won; Koo, Bon Seok; Lee, Dong Hyun

2014-06-01

The pyrolysis of Scenedesmus sp. and Jatropha seedshell cake (JSC) was investigated under similar operating condition in a fluidized bed reactor for comparison of pyrolytic behaviors from different species of lipids-containing biomass. Microalgae showed a narrower main peak in differential thermogravimetric curve compared to JSC due to different constituents. Pyrolysis liquid yields were similar; liquid's oil proportion of microalgae is higher than JSC. Microalgae bio-oil was characterized by similar carbon and hydrogen contents and higher H/C and O/C molar ratios compared to JSC due to compositional difference. The pyrolytic oils from microalgae and JSC contained more oxygen and nitrogen and less sulfur than petroleum and palm oils. The pyrolytic oils showed high yields of fatty oxygenates and nitrogenous compounds. The microalgae bio-oil features in high concentrations of aliphatic compounds, fatty acid alkyl ester, alcohols and nitriles. Microalgae showed potentials for alternative feedstock for green diesel, and commodity and valuable chemicals. Copyright © 2014 Elsevier Ltd. All rights reserved.

Comparative evaluation of chemical and enzymatic saccharification of mixotrophically grown de-oiled microalgal biomass for reducing sugar production.

PubMed

Pancha, Imran; Chokshi, Kaumeel; Maurya, Rahulkumar; Bhattacharya, Sourish; Bachani, Pooja; Mishra, Sandhya

2016-03-01

For the commercialization of microalgal based biofuels, utilization of de-oiled carbohydrate rich biomass is important. In the present study, chemo-enzymatic hydrolysis of mixotrophically grown Scenedesmus sp. CCNM 1077 de-oiled biomass is evaluated. Among the chemical hydrolysis, use of 0.5M HCl for 45 min at 121°C resulted in highest saccharification yield of 37.87% w/w of de-oiled biomass. However, enzymatic hydrolysis using Viscozyme L at loading rate of 20 FBGU/g of de-oiled biomass, pH 5.5 and temperature 45°C for 72 h resulted in saccharification yield of 43.44% w/w of de-oiled biomass. Further, 78% ethanol production efficiency was achieved with enzymatically hydrolyzed de-oiled biomass using yeast Saccharomyces cerevisiae ATCC 6793. These findings of the present study show application of mixotrophically grown de-oiled biomass of Scenedesmus sp. CCNM 1077 as promising feedstock for bioethanol production. Copyright © 2016 Elsevier Ltd. All rights reserved.

Performance, blood parameters and meat yield in broiler chickens supplemented with Mexican oregano oil

USDA-ARS?s Scientific Manuscript database

This study was conducted to evaluate the inclusion of Mexican oregano oil (MOO) Lippia berlandieri Schauer in broiler diets during grow-out on performance, blood parameters, and meat yield. One hundred and sixty-two one-day-old broilers, randomly divided into three equal groups (treatments): CON =...

Transesterification of Waste Olive Oil by "Candida" Lipase

ERIC Educational Resources Information Center

Shen, Xiangping; Vasudevan, Palligarnai T.

2008-01-01

Biodiesel was produced by transesterification of waste olive oil with methanol and Novozym [R] 435. The effect of the molar ratio of methanol to triolein, mode of methanol addition, reaction temperature, and mixing speed on biodiesel yield was determined. The effect of different acyl acceptors and/or solvents on biodiesel yield was also evaluated.…

Structural Properties of a Sheared Dense Emulsion

NASA Astrophysics Data System (ADS)

Dutta, S. K.; Knowlton, E. D.; Blair, D. L.

2011-03-01

The flow of a compressed emulsion above its yield point can be described by a velocity profile in addition to a rearrangement of individual droplets on top of this time averaged motion. Using a confocal microscope, we have tracked the droplets of an oil-in-water emulsion as they are sheared in a rheometer. When the applied stress is large, the velocity profile shows a nearly affine deformation, while there is strong strain localization close to yield. The crossover between these two behaviors occurs at higher shear rates as the volume fraction of the droplets is increased. At shorter length scales, rearrangement events are heterogeneously distributed, reflecting the disordered packing of the emulsion droplets. This characterization is a step towards linking bulk viscoelastic properties to local structural relaxation as the system leaves the jammed state. This work is funded by the NSF through Grant DMR 0847490.

Process Intensification of Enzymatic Fatty Acid Butyl Ester Synthesis Using a Continuous Centrifugal Contactor Separator.

PubMed

Ilmi, Miftahul; Abduh, Muhammad Y; Hommes, Arne; Winkelman, Jozef G M; Hidayat, Chusnul; Heeres, Hero J

2018-01-17

Fatty acid butyl esters were synthesized from sunflower oil with 1-butanol using a homogeneous Rhizomucor miehei lipase in a biphasic organic (triglyceride, 1-butanol, hexane)- water (with enzyme) system in a continuous setup consisting of a cascade of a stirred tank reactor and a continuous centrifugal contactor separator (CCCS), the latter being used for integrated reaction and liquid-liquid separation. A fatty acid butyl ester yield up to 93% was obtained in the cascade when operated in a once-through mode. The cascade was run for 8 h without operational issues. Enzyme recycling was studied by reintroduction of the water phase from the CCCS outlet to the stirred tank reactor. Product yield decreased over time to an average of 50% of the initial value, likely due to accumulation of 1-butanol in water phase, loss of enzyme due to agglomeration, and the formation of a separate enzyme layer.

A heterogeneous landscape does not guarantee high crop pollination

PubMed Central

Hambäck, Peter A.; Lemessa, Debissa; Nemomissa, Sileshi; Hylander, Kristoffer

2016-01-01

The expansion of pollinator-dependent crops, especially in the developing world, together with reports of worldwide pollinator declines, raises concern of possible yield gaps. Farmers directly reliant on pollination services for food supply often live in regions where our knowledge of pollination services is poor. In a manipulative experiment replicated at 23 sites across an Ethiopian agricultural landscape, we found poor pollination services and severe pollen limitation in a common oil crop. With supplementary pollination, the yield increased on average by 91%. Despite the heterogeneous agricultural matrix, we found a low bee abundance, which may explain poor pollination services. The variation in pollen limitation was unrelated to surrounding forest cover, local bee richness and bee abundance. While practices that commonly increase pollinators (restricted pesticide use, flower strips) are an integral part of the landscape, these elements are apparently insufficient. Management to increase pollination services is therefore in need of urgent investigation. PMID:27629036

Process Intensification of Enzymatic Fatty Acid Butyl Ester Synthesis Using a Continuous Centrifugal Contactor Separator

PubMed Central

2017-01-01

Fatty acid butyl esters were synthesized from sunflower oil with 1-butanol using a homogeneous Rhizomucor miehei lipase in a biphasic organic (triglyceride, 1-butanol, hexane)– water (with enzyme) system in a continuous setup consisting of a cascade of a stirred tank reactor and a continuous centrifugal contactor separator (CCCS), the latter being used for integrated reaction and liquid–liquid separation. A fatty acid butyl ester yield up to 93% was obtained in the cascade when operated in a once-through mode. The cascade was run for 8 h without operational issues. Enzyme recycling was studied by reintroduction of the water phase from the CCCS outlet to the stirred tank reactor. Product yield decreased over time to an average of 50% of the initial value, likely due to accumulation of 1-butanol in water phase, loss of enzyme due to agglomeration, and the formation of a separate enzyme layer. PMID:29398779

Analysis of parameter and interaction between parameter of the microwave assisted transesterification process of coconut oil using response surface methodology

NASA Astrophysics Data System (ADS)

Hidayanti, Nur; Suryanto, A.; Qadariyah, L.; Prihatini, P.; Mahfud, Mahfud

2015-12-01

A simple batch process was designed for the transesterification of coconut oil to alkyl esters using microwave assisted method. The product with yield above 93.225% of alkyl ester is called the biodiesel fuel. Response surface methodology was used to design the experiment and obtain the maximum possible yield of biodiesel in the microwave-assisted reaction from coconut oil with KOH as the catalyst. The results showed that the time reaction and concentration of KOH catalyst have significant effects on yield of alkyl ester. Based on the response surface methodology using the selected operating conditions, the time of reaction and concentration of KOH catalyst in transesterification process were 150 second and 0.25%w/w, respectively. The largest predicted and experimental yield of alkyl esters (biodiesel) under the optimal conditions are 101.385% and 93.225%, respectively. Our findings confirmed the successful development of process for the transesterification reaction of coconut oil by microwave-assisted heating, which is effective and time-saving for alkyl ester production.

Fast microwave-assisted catalytic co-pyrolysis of corn stover and scum for bio-oil production with CaO and HZSM-5 as the catalyst.

PubMed

Liu, Shiyu; Xie, Qinglong; Zhang, Bo; Cheng, Yanling; Liu, Yuhuan; Chen, Paul; Ruan, Roger

2016-03-01

This study investigated fast microwave-assisted catalytic co-pyrolysis of corn stover and scum for bio-oil production with CaO and HZSM-5 as the catalyst. Effects of reaction temperature, CaO/HZSM-5 ratio, and corn stover/scum ratio on co-pyrolysis product fractional yields and selectivity were investigated. Results showed that co-pyrolysis temperature was selected as 550°C, which provides the maximum bio-oil and aromatic yields. Mixed CaO and HZSM-5 catalyst with the weight ratio of 1:4 increased the aromatic yield to 35.77 wt.% of feedstock, which was 17% higher than that with HZSM-5 alone. Scum as the hydrogen donor, had a significant synergistic effect with corn stover to promote the production of bio-oil and aromatic hydrocarbons when the H/C(eff) value exceeded 1. The maximum yield of aromatic hydrocarbons (29.3 wt.%) were obtained when the optimal corn stover to scum ratio was 1:2. Copyright © 2016 Elsevier Ltd. All rights reserved.

Effect of filter media size, mass flow rate and filtration stage number in a moving-bed granular filter on the yield and properties of bio-oil from fast pyrolysis of biomass.

PubMed

Paenpong, Chaturong; Inthidech, Sudsakorn; Pattiya, Adisak

2013-07-01

Fast pyrolysis of cassava rhizome was performed in a bench-scale fluidised-bed reactor unit incorporated with a cross-flow moving-bed granular filter. The objective of this research was to examine several process parameters including the granular size (425-1160 μm) and mass flow rate (0-12 g/min) as well as the number of the filtration stages (1-2 stages) on yields and properties of bio-oil. The results showed that the bio-oil yield decreased from 57.7 wt.% to 42.0-49.2 wt.% when increasing the filter media size, the mass flow rate and the filtration stage number. The effect of the process parameters on various properties of bio-oil is thoroughly discussed. In general, the bio-oil quality in terms of the solids content, ash content, initial viscosity, viscosity change and ageing rate could be enhanced by the hot vapour granular filtration. Therefore, bio-oil of high stability could be produced by the pyrolysis reactor configuration designed in this work. Copyright © 2013 Elsevier Ltd. All rights reserved.

In Situ Biosurfactant Production by Bacillus Strains Injected into a Limestone Petroleum Reservoir▿

PubMed Central

Youssef, N.; Simpson, D. R.; Duncan, K. E.; McInerney, M. J.; Folmsbee, M.; Fincher, T.; Knapp, R. M.

2007-01-01

Biosurfactant-mediated oil recovery may be an economic approach for recovery of significant amounts of oil entrapped in reservoirs, but evidence that biosurfactants can be produced in situ at concentrations needed to mobilize oil is lacking. We tested whether two Bacillus strains that produce lipopeptide biosurfactants can metabolize and produce their biosurfactants in an oil reservoir. Five wells that produce from the same Viola limestone formation were used. Two wells received an inoculum (a mixture of Bacillus strain RS-1 and Bacillus subtilis subsp. spizizenii NRRL B-23049) and nutrients (glucose, sodium nitrate, and trace metals), two wells received just nutrients, and one well received only formation water. Results showed in situ metabolism and biosurfactant production. The average concentration of lipopeptide biosurfactant in the produced fluids of the inoculated wells was about 90 mg/liter. This concentration is approximately nine times the minimum concentration required to mobilize entrapped oil from sandstone cores. Carbon dioxide, acetate, lactate, ethanol, and 2,3-butanediol were detected in the produced fluids of the inoculated wells. Only CO2 and ethanol were detected in the produced fluids of the nutrient-only-treated wells. Microbiological and molecular data showed that the microorganisms injected into the formation were retrieved in the produced fluids of the inoculated wells. We provide essential data for modeling microbial oil recovery processes in situ, including growth rates (0.06 ± 0.01 h−1), carbon balances (107% ± 34%), biosurfactant production rates (0.02 ± 0.001 h−1), and biosurfactant yields (0.015 ± 0.001 mol biosurfactant/mol glucose). The data demonstrate the technical feasibility of microbial processes for oil recovery. PMID:17172458

Analyses of Hypomethylated Oil Palm Gene Space

PubMed Central

Jayanthi, Nagappan; Mohd-Amin, Ab Halim; Azizi, Norazah; Chan, Kuang-Lim; Maqbool, Nauman J.; Maclean, Paul; Brauning, Rudi; McCulloch, Alan; Moraga, Roger; Ong-Abdullah, Meilina; Singh, Rajinder

2014-01-01

Demand for palm oil has been increasing by an average of ∼8% the past decade and currently accounts for about 59% of the world's vegetable oil market. This drives the need to increase palm oil production. Nevertheless, due to the increasing need for sustainable production, it is imperative to increase productivity rather than the area cultivated. Studies on the oil palm genome are essential to help identify genes or markers that are associated with important processes or traits, such as flowering, yield and disease resistance. To achieve this, 294,115 and 150,744 sequences from the hypomethylated or gene-rich regions of Elaeis guineensis and E. oleifera genome were sequenced and assembled into contigs. An additional 16,427 shot-gun sequences and 176 bacterial artificial chromosomes (BAC) were also generated to check the quality of libraries constructed. Comparison of these sequences revealed that although the methylation-filtered libraries were sequenced at low coverage, they still tagged at least 66% of the RefSeq supported genes in the BAC and had a filtration power of at least 2.0. A total 33,752 microsatellites and 40,820 high-quality single nucleotide polymorphism (SNP) markers were identified. These represent the most comprehensive collection of microsatellites and SNPs to date and would be an important resource for genetic mapping and association studies. The gene models predicted from the assembled contigs were mined for genes of interest, and 242, 65 and 14 oil palm transcription factors, resistance genes and miRNAs were identified respectively. Examples of the transcriptional factors tagged include those associated with floral development and tissue culture, such as homeodomain proteins, MADS, Squamosa and Apetala2. The E. guineensis and E. oleifera hypomethylated sequences provide an important resource to understand the molecular mechanisms associated with important agronomic traits in oil palm. PMID:24497974

Enhanced yield of phenolic extracts from banana peels (Musa acuminata Colla AAA) and cinnamon barks (Cinnamomum varum) and their antioxidative potentials in fish oil.

PubMed

Anal, Anil Kumar; Jaisanti, Sirorat; Noomhorm, Athapol

2014-10-01

The bioactive compounds of banana peels and cinnamon barks were extracted by vacuum microwave and ultrasonic-assisted extraction methods at pre-determined temperatures and times. These methods enhance the yield extracts in shorter time. The highest yields of both extracts were obtained from the conditions which employed the highest temperature and the longest time. The extracts' yield from cinnamon bark method was higher by ultrasonic than vacuum microwave method, while vacuum microwave method gave higher extraction yield from banana peel than ultrasonic method. The phenolic contents of cinnamon bark and banana peel extracts were 467 and 35 mg gallic acid equivalent/g extract, respectively. The flavonoid content found in banana peel and cinnamon bark extracts were 196 and 428 mg/g quercetin equivalent, respectively. In addition, it was found that cinnamon bark gave higher 2,2-Diphenyl-1-1 picryhydrazyl (DPPH) radical scavenging activity and total antioxidant activity (TAA). The antioxidant activity of the extracts was analyzed by measuring the peroxide and p-anisidine values after oxidation of fish oils, stored for a month (30 days) at 25 °C and showed lesser peroxide and p-anisidine values in the fish oils containing the sample extracts in comparison to the fish oil without containing any extract. The banana peel and cinnamon extracts had shown the ability as antioxidants to prevent the oxidation of fish oil and might be considered as rich sources of natural antioxidant.

Characterization of Chemically and Thermally Treated Oil-in-Water Heteroaggregates and Comparison to Conventional Emulsions.

PubMed

Maier, Christiane; Reichert, Corina L; Weiss, Jochen

2016-10-01

Heteroaggregated oil-in-water (O/W) emulsions formed by targeted combination of oppositely charged emulsion droplets were proposed to be used for the modulation of physical properties of food systems, ideally achieving the formation of a particulate 3-dimensional network at comparably low-fat content. In this study, rheological properties of Quillaja saponins (QS), sugar beet pectin (SBP), and whey protein isolate (WPI) stabilized conventional and heteroaggregated O/W emulsions at oil contents of 10% to 60% (w/w) were investigated. Selected systems having an oil content of 30% (w/w) and different particle sizes (d 43 ≤ 1.1 or ≥16.7 μm) were additionally subjected to chemical (genipin or glutaraldehyde) and thermal treatments, aiming to increase network stability. Subsequently, their rheological properties and stability were assessed. Yield stresses (τ 0 ) of both conventional and heteroaggregated O/W emulsions were found to depend on emulsifier type, oil content, and initial droplet size. For conventional emulsions, high yield stresses were only observed for SBP-based emulsions (τ 0 , SBP approximately 157 Pa). Highest yield stresses of heteroaggregates were observed when using small droplets stabilized by SBP/WPI (approximately 15.4 Pa), being higher than those of QS/WPI (approximately 1.6 Pa). Subsequent treatments led to significant alterations in rheological properties for SBP/WPI systems, with yield stresses increasing 29-fold (glutaraldehyde) and 2-fold (thermal treatment) compared to untreated heteroaggregates, thereby surpassing yield stresses of similarly treated conventional SBP emulsions. Genipin-driven treatments proved to be ineffective. Results should be of interest to food manufacturers wishing to design viscoelastic food emulsion based systems at lower oil droplet contents. © 2016 Institute of Food Technologists®.

Hydrothermal liquefaction pathways for low-nitrogen biocrude from wet algae

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tanzella, Francis; Lim, Jin-Ping

Our SRI International (SRI) team has developed a new two-step hydrothermal liquefaction (HTL) process to convert wet algal biomass into biocrude oil. The first step in the process (low-temperature HTL or HTL1) yields crude oil but, most importantly, it selectively dissolves nitrogen-containing compounds in the aqueous phase. Once the oil and the aqueous phase are separated, the low-nitrogen soft solids left behind can be taken to the second step (high-temperature HTL or HTL2) for full conversion to biocrude. HTL2 will hence yield low-nitrogen biocrude, which can be hydro-processed to yield transportation fuels. The expected high carbon yield and low nitrogenmore » content can lead to a transportation fuel from algae that avoids two problems common to existing algae-to-fuel processes: (1) poisoning of the hydro-processing catalyst; and (2) inefficient conversion of algae-to-liquid fuels. The process we studied would yield a new route to strategic energy production from domestic sources.« less

Reciprocal combinations of barley and corn grains in oil-supplemented diets: feeding behavior and milk yield of lactating cows.

PubMed

Kargar, S; Ghorbani, G R; Khorvash, M; Sadeghi-Sefidmazgi, A; Schingoethe, D J

2014-11-01

The effect of barley-based (BBD) or corn-based diets (CBD), or their equal blend (BCBD) on dry matter (DM) intake, feeding and chewing behavior, and production performance of lactating dairy cows was evaluated. Nine multiparous Holstein cows (75.6 ± 11.0 d in milk) were used in a triplicate 3 × 3 Latin square design with 21-d periods. Forage-to-concentrate ratio (40:60), forage neutral detergent fiber (20% of DM), total neutral detergent fiber (>29% of DM), and geometric mean particle size (4.3mm) were similar among treatments. Meal patterns, including meal size and intermeal interval, were not affected by the dietary treatments and DM intake (25.6 kg/d) was not different among treatments. Ether extract intake increased linearly with increasing amount of the corn grain in the diets. Due to similar feed intake, actual milk (48.6 kg/d), 4% fat-corrected milk (36.8 kg/d), and fat- and protein-corrected milk (38.1 kg/d) yields were not affected by treatments. Average milk protein percentage and yield were 2.83% and 1.37 kg/d, respectively, and were not different across treatments. Milk fat percentage increased linearly with increasing amount of corn grain in the diets and was greater in CBD relative to BCBD but not BBD (2.31, 2.28, and 2.57%, for BBD, BCBD, and CBD, respectively). However, milk fat yield tended to show a linear increase as the amount of corn grain included in the diets increased. Results indicated that changing diet fermentability by replacing barley grain for corn grain in oil-supplemented diets did not influence feeding patterns and thereby no changes in feed intake and milk yield occurred. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Rethink potential risks of toxic emissions from natural gas and oil mining.

PubMed

Meng, Qingmin

2018-09-01

Studies have showed the increasing environmental and public health risks of toxic emissions from natural gas and oil mining, which have become even worse as fracking is becoming a dominant approach in current natural gas extraction. However, governments and communities often overlook the serious air pollutants from oil and gas mining, which are often quantified lower than the significant levels of adverse health effects. Therefore, we are facing a challenging dilemma: how could we clearly understand the potential risks of air toxics from natural gas and oil mining. This short study aims at the design and application of simple and robust methods to enhance and improve current understanding of the becoming worse toxic air emissions from natural gas and oil mining as fracking is becoming the major approach. Two simple ratios, the min-to-national-average and the max-to-national-average, are designed and applied to each type of air pollutants in a natural gas and oil mining region. The two ratios directly indicate how significantly high a type of air pollutant could be due to natural gas and oil mining by comparing it to the national average records, although it may not reach the significant risks of adverse health effects according to current risk screening methods. The min-to-national-average and the max-to-national-average ratios can be used as a direct and powerful method to describe the significance of air pollution by comparing it to the national average. The two ratios are easy to use for governments, stakeholders, and the public to pay enough attention on the air pollutants from natural gas and oil mining. The two ratios can also be thematically mapped at sampled sites for spatial monitoring, but spatial mitigation and analysis of environmental and health risks need other measurements of environmental and demographic characteristics across a natural gas and oil mining area. Copyright © 2018 Elsevier Ltd. All rights reserved.

Distillation time alters essential oil yield, composition, and antioxidant activity of male Juniperus scopulorum trees.

PubMed

Zheljazkov, Valtcho D; Astatkie, Tess; Jeliazkova, Ekaterina A; Schlegel, Vicki

2012-01-01

The objective of this study was to evaluate the effect of 15 distillation times (DT), ranging from 1.25 to 960 min, on oil yield, essential oil profiles, and antioxidant capacity of male J. scopulorum trees. Essential oil yields were 0.07% at 1.25 min DT and reached a maximum of 1.48% at 840 min DT. The concentrations of alpha-thujene (1.76-2.75%), alpha-pinene (2.9-8.7%), sabinene (45-74.7%), myrcene (2.4-3.4%), and para-cymene (0.8-3.1%) were highest at the shortest DT (1.5 to 5 min) and decreased with increasing DT. Cis-sabinene hydrate (0.5-0.97%) and linalool plus trans-sabinene (0.56-1.6%) reached maximum levels at 40 min DT. Maximum concentrations of limonene (2.3-2.8%) and pregeijerene-B (0.06-1.4%) were obtained at 360-480 min DT, and 4-terpinenol (0.7-5.7%) at 480 min DT. Alpha-terpinene (0.16-2.9%), gamma-terpinene (0.3-4.9%) and terpinolene (0.3-1.4%) reached maximum at 720 min DT. The concentrations of delta-cadinene (0.06-1.65%), elemol (0-6.0%), and 8-alpha-acetoxyelemol (0-4.4%) reached maximum at 840 min DT. The yield of the essential oil constituents increased with increasing DT. Only linalool/transsabinene hydrate reached a maximum yield at 360 min DT. Maximum yields of the following constituents were obtained at 720 min DT: alpha-thujene, alpha-pinene, camphene, sabinene, myrcene, alpha-terpinene, para-cimene, limonene, gamma-terpinene, terpinolene, and 4-terpinenol. At 840 min DT, cis-sabinene hydrate, prejeijerene-B, gamma muurolene, delta-cadinene, reached maximum. At 960 min DT, maximum yields of beta-pinene, elemol, alphaeudesmol/betaeudesmol, 8-alpha-acetoxyelemol were reached. These changes were adequately modeled by either the Michaelis-Menten or the Power (Convex) nonlinear regression models. Oils from the 480 min DT showed higher antioxidant activity compared to samples collected at 40, 160, or 960 min DT. These results show the potential for obtaining essential oils with various compositions and antioxidant capacity from male J. scopulorum by varying DT. This study can be used as a reference paper for comparing results of reports where different lengths of the DT were used.

Hydrodistillation time affects dill seed essential oil yield, composition, and bioactivity

USDA-ARS?s Scientific Manuscript database

Dill (Anethum graveolens L.) essential oil is widely used by the food and pharmaceutical industries. We hypothesized that the chemical constituents of dill seed essential oil are eluted at different times during the hydrodistillation process, resulting in oils with different composition and bioactiv...

Replacement of dietary fish oil with vegetable oils improves the growth and flesh quality of large yellow croaker ( Larmichthys crocea)

NASA Astrophysics Data System (ADS)

Duan, Qingyuan; Mai, Kangsen; Shentu, Jikang; Ai, Qinghui; Zhong, Huiying; Jiang, Yujian; Zhang, Lu; Zhang, Chunxiao; Guo, Sitong

2014-06-01

We investigated the effect of the replacement of dietary fish oil with vegetable oils on the growth and flesh quality of large yellow croaker ( Larmichthys crocea). The basal diet (FO) was formulated to contain 66.5% fish meal and 6.4% menhaden fish oil; whereas the other 3 experimental diets were formulated by replacing the fish oil with 50% soybean oil (SO50), 100% soybean oil (SO100) and 100% palm oil (PO100), respectively. The 4 diets were randomly assigned to 4 floating sea cages (3.0 m × 3.0 m × 3.0 m), and each was stocked with 250 fish individuals with an initial average weight of 245.29 g ± 7.45 g. The fish were fed to apparent satiation twice a day at 5:00 and 17:00, respectively, for 12 weeks. Experimental analysis showed that the specific growth rate of fish fed SO50 or PO100 were significantly higher than that of fish fed FO or SO100 ( P<0.05), and crude lipid contents of ventral muscle and viscera were significantly lower in fish fed FO than in those fed the other 3 diets ( P<0.05). No significant differences in condition factor, viscerosomatic index, hepatosomatic index, gutted yield and colorimetric values of fish among the dietary treatments were observed ( P>0.05). Compared to FO diet, SO50, SO100 and PO100 diets led to substantial decreases in the liquid loss and water loss from fresh fillets (1 d, 4°C) ( P<0.05). Similarly, thiobarbituric acid reactive substance (TBARS) values of fillets under different storage conditions (1 d, 4°C; 7 d, 4°C; 4 weeks, -20°C; 8 weeks, -20°C) decreased significantly after partial or complete replacement of fish oil with vegetable oils. These findings indicated that the growth performance and selected flesh quality properties (liquid holding capacity and TBARS value) of large yellow croaker were substantially improved by replacing dietary fish oil with vegetable oils.

Extraction and identification of bioactive compounds from agarwood leaves

NASA Astrophysics Data System (ADS)

Lee, N. Y.; Yunus, M. A. C.; Idham, Z.; Ruslan, M. S. H.; Aziz, A. H. A.; Irwansyah, N.

2016-11-01

Agarwood commonly known as gaharu, aloeswood or eaglewood have been used as traditional medicine for centuries and its essential oil also being used as perfumery ingredients and aroma enhancers in food products. However, there is least study on the agarwood leaves though it contains large number of biomolecules component that show diverse pharmacological activity. Previous study showed that the extracted compounds from the leaves possess activities like anti-mutagenic, anti-tumor and anti-helminthic. The main objectives of this research were to determine bioactive compounds in agarwood leaves; leaves extract and oil yield obtained from maceration and soxhlet extraction methods respectively. The maceration process was performed at different operating temperature of 25°C, 50°C and 75°C and different retention time at 30, 60, 90 and 120 minutes. Meanwhile, various solvents were used to extract the oil from agarwood leaves using soxhlet method which are hexane, water, isopropanol and ethanol. The extracted oil from agarwood leaves by soxhlet extraction was analyzed using gas chromatography mass spectrometry. The results showed that the highest extract of 1.53% was obtained when increase the temperature to 75 °C and longest retention time of 120 minutes gave the highest oil yield of 2.10 % by using maceration. This is because at higher temperature enhances the solubility solute and diffusivity coefficient, thus increase the extract yield while longer retention time allow the reaction between solvent and solute occurred more rapidly giving higher extract. Furthermore, the soxhlet extraction using n-hexane as the solvent gave the highest oil yield as compared to other solvent due to the non-polar properties of n-hexane increase the efficiency of oil which is also non-polar to soluble in the solvent. In addition, the results also reported that the oil extracted from agarwood leaves contains bioactive compounds which are phytol, squalene, n-hexadecanoic acid and octadecatrienoic acid. Therefore, oil extracted from agarwood leaves has the potential to be applied in food, pharmaceutical, nutraceutical and cosmetics industries.

A modified approach for isolation of essential oil from fruit of Amorpha fruticosa Linn using microwave-assisted hydrodistillation concatenated liquid-liquid extraction.

PubMed

Chen, Fengli; Jia, Jia; Zhang, Qiang; Gu, Huiyan; Yang, Lei

2017-11-17

In this work, a modified technique was developed to separate essential oil from the fruit of Amorpha fruticosa using microwave-assisted hydrodistillation concatenated liquid-liquid extraction (MHD-LLE). The new apparatus consists of two series-wound separation columns for separating essential oil, one is the conventional oil-water separation column, and the other is the extraction column of components from hydrosol using an organic solvent. Therefore, the apparatus can simultaneously collect the essential oil separated on the top of hydrosol and the components extracted from hydrosol using an organic solvent. Based on the yield of essential oil in the first and second separation columns, the effects of parameters were investigated by single factor experiments and Box-Behnken design. Under the optimum conditions (2mL ethyl ether as the extraction solvent in the second separation column, 12mL/g liquid-solid ratio, 4.0min homogenate time, 35min microwave irradiation time and 540W microwave irradiation power), satisfactory yields for the essential oil in the first separation column (10.31±0.33g/kg) and second separation column (0.82±0.03g/kg) were obtained. Compared with traditional methods, the developed method gave a higher yield of essential oil in a shorter time. In addition, GC-MS analysis of the essential oil indicated significant differences of the relative contents of individual volatile components in the essential oils obtained in the two separation columns. Therefore, the MHD-LLE technique developed here is a good alternative for the isolation of essential oil from A. fruticosa fruit as well as other herbs. Copyright © 2017 Elsevier B.V. All rights reserved.

Inhibitory effect of essential oils obtained from plants grown in Colombia on yellow fever virus replication in vitro.

PubMed

Meneses, Rocío; Ocazionez, Raquel E; Martínez, Jairo R; Stashenko, Elena E

2009-03-06

An antiviral drug is needed for the treatment of patients suffering from yellow fever. Several compounds present in plants can inactive in vitro a wide spectrum of animal viruses. In the present study the inhibitory effect of essential oils of Lippia alba, Lippia origanoides, Oreganum vulgare and Artemisia vulgaris on yellow fever virus (YFV) replication was investigated. The cytotoxicity (CC(50)) on Vero cells was evaluated by the MTT reduction method. The minimum concentration of the essential oil that inhibited virus titer by more than 50% (MIC) was determined by virus yield reduction assay. YFV was incubated 24 h at 4 degrees C with essential oil before adsorption on Vero cell, and viral replication was carried out in the absence or presence of essential oil. Vero cells were exposed to essential oil 24 h at 37 degrees C before the adsorption of untreated-virus. The CC(50) values were less than 100 microg/mL and the MIC values were 3.7 and 11.1 microg/mL. The CC(50)/MIC ratio was of 22.9, 26.4, 26.5 and 8.8 for L. alba, L origanoides, O. vulgare and A. vulgaris, respectively. The presence of essential oil in the culture medium enhances the antiviral effect: L. origanoides oil at 11.1 microg/mL produced a 100% reduction of virus yield, and the same result was observed with L. alba, O. vulgare and A. vulgaris oils at 100 microg/mL. No reduction of virus yield was observed when Vero cells were treated with essential oil before the adsorption of untreated-virus. The essential oils evaluated in the study showed antiviral activities against YFV. The mode of action seems to be direct virus inactivation.

Pyrolysis and kinetic analyses of a perennial grass (Saccharum ravannae L.) from north-east India: Optimization through response surface methodology and product characterization.

PubMed

Saikia, Ruprekha; Baruah, Bhargav; Kalita, Dipankar; Pant, Kamal K; Gogoi, Nirmali; Kataki, Rupam

2018-04-01

The objective of the present investigation was to optimize the pyrolysis condition of an abundantly available and low cost perennial grass of north-east India Saccharum ravannae L. (S. ravannae) using response surface methodology based on central composite design. Kinetic study of the biomass was conducted at four different heating rates of 10, 20, 40 and 60 °C min -1 and results were interpreted by Friedman, Kissinger Akira Sunnose and Flynn-Wall-Ozawa methods. Average activation energy 151.45 kJ mol -1 was used for evaluation of reaction mechanism following Criado master plot. Maximum bio-oil yield of 38.1 wt% was obtained at pyrolysis temperature of 550 °C, heating rate of 20 °C min -1 and nitrogen flow rate of 226 mL min -1 . Study on bio-oil quality revealed higher content of hydrocarbon, antioxidant property, total phenolic content and metal chelating capacity. These opened up probable applications of S. ravannae bio-oil in different fields including fuel, food industry and biomedical domain. Copyright © 2018 Elsevier Ltd. All rights reserved.

On the modeling of the 2010 Gulf of Mexico Oil Spill

NASA Astrophysics Data System (ADS)

Mariano, A. J.; Kourafalou, V. H.; Srinivasan, A.; Kang, H.; Halliwell, G. R.; Ryan, E. H.; Roffer, M.

2011-09-01

Two oil particle trajectory forecasting systems were developed and applied to the 2010 Deepwater Horizon Oil Spill in the Gulf of Mexico. Both systems use ocean current fields from high-resolution numerical ocean circulation model simulations, Lagrangian stochastic models to represent unresolved sub-grid scale variability to advect oil particles, and Monte Carlo-based schemes for representing uncertain biochemical and physical processes. The first system assumes two-dimensional particle motion at the ocean surface, the oil is in one state, and the particle removal is modeled as a Monte Carlo process parameterized by a one number removal rate. Oil particles are seeded using both initial conditions based on observations and particles released at the location of the Maconda well. The initial conditions (ICs) of oil particle location for the two-dimensional surface oil trajectory forecasts are based on a fusing of all available information including satellite-based analyses. The resulting oil map is digitized into a shape file within which a polygon filling software generates longitude and latitude with variable particle density depending on the amount of oil present in the observations for the IC. The more complex system assumes three (light, medium, heavy) states for the oil, each state has a different removal rate in the Monte Carlo process, three-dimensional particle motion, and a particle size-dependent oil mixing model. Simulations from the two-dimensional forecast system produced results that qualitatively agreed with the uncertain "truth" fields. These simulations validated the use of our Monte Carlo scheme for representing oil removal by evaporation and other weathering processes. Eulerian velocity fields for predicting particle motion from data-assimilative models produced better particle trajectory distributions than a free running model with no data assimilation. Monte Carlo simulations of the three-dimensional oil particle trajectory, whose ensembles were generated by perturbing the size of the oil particles and the fraction in a given size range that are released at depth, the two largest unknowns in this problem. 36 realizations of the model were run with only subsurface oil releases. An average of these results yields that after three months, about 25% of the oil remains in the water column and that most of the oil is below 800 m.

Production of bio-oil rich in acetic acid and phenol from fast pyrolysis of palm residues using a fluidized bed reactor: Influence of activated carbons.

PubMed

Jeong, Jae-Yong; Lee, Uen-Do; Chang, Won-Seok; Jeong, Soo-Hwa

2016-11-01

In this study, palm residues were pyrolyzed in a bench-scale (3kg/h) fast pyrolysis plant equipped with a fluidized bed reactor and bio-oil separation system for the production of bio-oil rich in acetic acid and phenol. Pyrolysis experiments were performed to investigate the effects of reaction temperature and the types and amounts of activated carbon on the bio-oil composition. The maximum bio-oil yield obtained was approximately 47wt% at a reaction temperature of 515°C. The main compounds produced from the bio-oils were acetic acid, hydroxyacetone, phenol, and phenolic compounds such as cresol, xylenol, and pyrocatechol. When coal-derived activated carbon was applied, the acetic acid and phenol yields in the bio-oils reached 21 and 19wt%, respectively. Finally, bio-oils rich in acetic acid and phenol could be produced separately by using an in situ bio-oil separation system and activated carbon as an additive. Copyright © 2016 Elsevier Ltd. All rights reserved.

Microwave pyrolysis of distillers dried grain with solubles (DDGS) for biofuel production.

PubMed

Lei, Hanwu; Ren, Shoujie; Wang, Lu; Bu, Quan; Julson, James; Holladay, John; Ruan, Roger

2011-05-01

Microwave pyrolysis of distillers dried grain with solubles (DDGS) was investigated to determine the effects of pyrolytic conditions on the yields of bio-oil, syngas, and biochar. Pyrolysis process variables included reaction temperature, time, and power input. Microwave pyrolysis of DDGS was analyzed using response surface methodology to find out the effect of process variables on the biofuel (bio-oil and syngas) conversion yield and establish prediction models. Bio-oil recovery was in the range of 26.5-50.3 wt.% of the biomass. Biochar yields were 23.5-62.2% depending on the pyrolysis conditions. The energy content of DDGS bio-oils was 28 MJ/kg obtained at the 650°C and 8 min, which was about 66.7% of the heating value of gasoline. GC/MS analysis indicated that the biooil contained a series of important and useful chemical compounds: aliphatic and aromatic hydrocarbons. At least 13% of DDGS bio-oil was the same hydrocarbon compounds found in regular unleaded gasoline. Copyright © 2011 Elsevier Ltd. All rights reserved.

Ultrasound-assisted extraction and quantitation of oils from Syzygium aromaticum flower bud (clove) with supercritical carbon dioxide.

PubMed

Yang, Yu-Chiao; Wei, Ming-Chi; Hong, Show-Jen

2014-01-03

This study evaluated ultrasound-assisted supercritical carbon dioxide (USC-CO2) extraction for determining the extraction yields of oils and the contents of eugenol, β-caryophyllene, eugenyl acetate and α-humulene from clove buds. Compared to traditional SC-CO2 extraction, USC-CO2 extraction might provide a 13.5% increase in the extraction yield for the oil while utilizing less severe operating parameters, such as temperature, pressure, CO2 flow rate and the time consumed by the process. Our results were comparable to those obtained using the heat reflux extraction method, though the yield was improved by 20.8% using USC-CO2. In kinetic studies, the USC-CO2 extraction of clove oil followed second-order kinetics. The activation energy for the oil extraction was 76.56kJ/mol. The USC-CO2 procedure facilitated the use of mild extraction conditions, improved extraction efficiency and the quality of products and is a potential method for industry. Copyright © 2013 Elsevier B.V. All rights reserved.

Microwave pyrolysis of distillers dried grain with solubles (DDGS) for biofuel production

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lei, Hanwu; Ren, Shoujie; Wang, Lu

2011-05-01

Microwave pyrolysis of distillers dried grain with solubles (DDGS) was investigated to determine the effects of pyrolytic conditions on the yields of bio-oil, syngas, and biochar. Pyrolysis process variables included reaction temperature, time, and power input. Microwave pyrolysis of DDGS was analyzed using response surface methodology to find out the effect of process variables on the biofuel (bio-oil and syn- gas) conversion yield and establish prediction models. Bio-oil recovery was in the range of 26.5–50.3 wt.% of the biomass. Biochar yields were 23.5–62.2% depending on the pyrolysis conditions. The energy con- tent of DDGS bio-oils was 28 MJ/kg obtained atmore » the 650 oC and 8 min, which was about 66.7% of the heat- ing value of gasoline. GC/MS analysis indicated that the biooil contained a series of important and useful chemical compounds: aliphatic and aromatic hydrocarbons. At least 13% of DDGS bio-oil was the same hydrocarbon compounds found in regular unleaded gasoline.« less

Measurement of atmospheric pollutants associated with oil and natural gas exploration and production activity in Pennsylvania's Allegheny National Forest.

PubMed

Pekney, Natalie J; Veloski, Garret; Reeder, Matthew; Tamilia, Joseph; Rupp, Erik; Wetzel, Alan

2014-09-01

Oil and natural gas exploration and production (E&P) activities generate emissions from diesel engines, compressor stations, condensate tanks, leaks and venting of natural gas, construction of well pads, and well access roads that can negatively impact air quality on both local and regional scales. A mobile, autonomous air quality monitoring laboratory was constructed to collect measurements of ambient concentrations of pollutants associated with oil and natural gas E&P activities. This air-monitoring laboratory was deployed to the Allegheny National Forest (ANF) in northwestern Pennsylvania for a campaign that resulted in the collection of approximately 7 months of data split between three monitoring locations between July 2010 and June 2011. The three monitoring locations were the Kane Experimental Forest (KEF) area in Elk County, which is downwind of the Sackett oilfield; the Bradford Ranger Station (BRS) in McKean County, which is downwind of a large area of historic oil and gas productivity; and the U.S. Forest Service Hearts Content campground (HC) in Warren County, which is in an area relatively unimpacted by oil and gas development and which therefore yielded background pollutant concentrations in the ANF. Concentrations of criteria pollutants ozone and NO2 did not vary significantly from site to site; averages were below National Ambient Air Quality Standards. Concentrations of volatile organic compounds (VOCs) associated with oil and natural gas (ethane, propane, butane, pentane) were highly correlated. Applying the conditional probability function (CPF) to the ethane data yielded most probable directions of the sources that were coincident with known location of existing wells and activity. Differences between the two impacted and one background site were difficult to discern, suggesting the that the monitoring laboratory was a great enough distance downwind of active areas to allow for sufficient dispersion with background air such that the localized plumes were not detected. Implications: Monitoring of pollutants associated with oil and natural gas exploration and production activity at three sites within the Allegheny National Forest (ANF) showed only slight site-to-site differences even with one site far removed from these activities. However, the impact was evident not in detection of localized plumes but in regional elevated ethane concentrations, as ethane can be considered a tracer species for oil and natural gas activity. The data presented serve as baseline conditions for evaluation of impacts from future development of Marcellus or Utica shale gas reserves.

Effect of high oleic acid soybean on seed oil, protein concentration, and yield

USDA-ARS?s Scientific Manuscript database

Soybeans with high oleic acid content are desired by oil processors because of their improved oxidative stability for broader use in food, fuel and other products. However, non-GMO high-oleic soybeans have tended to have low seed yield. The objective of this study was to test non-GMO, high-oleic s...

Optimisation of steam distillation extraction oil from onion by response surface methodology and its chemical composition.

PubMed

Wang, Zhao Dan; Li, Li Hua; Xia, Hui; Wang, Feng; Yang, Li Gang; Wang, Shao Kang; Sun, Gui Ju

2018-01-01

Oil extraction from onion was performed by steam distillation. Response surface methodology was applied to evaluate the effects of ratio of water to raw material, extraction time, zymolysis temperature and distillation times on yield of onion oil. The maximum extraction yield (1.779%) was obtained as following conditions: ratio of water to raw material was 1, extraction time was 2.5 h, zymolysis temperature was 36° and distillation time was 2.6 h. The experimental values agreed well with those predicted by regression model. The chemical composition of extracted onion oil under the optimum conditions was analysed by gas chromatography-mass spectrometry technology. The results showed that sulphur compounds, like alkanes, sulphide, alkenes, ester and alcohol, were the major components of onion oil.

Fast, Low-Cost and Non-Destructive Physico-Chemical Analysis of Virgin Olive Oils Using Near-Infrared Reflectance Spectroscopy

PubMed Central

Garrido-Varo, Ana; Sánchez, María-Teresa; De la Haba, María-José; Torres, Irina; Pérez-Marín, Dolores

2017-01-01

Near-Infrared (NIR) Spectroscopy was used for the non-destructive assessment of physico-chemical quality parameters in olive oil. At the same time, the influence of the sample presentation mode (spinning versus static cup) was evaluated using two spectrophotometers with similar optical characteristics. A total of 478 olive oil samples were used to develop calibration models, testing various spectral signal pre-treatments. The models obtained by applying MPLS regression to spectroscopic data yielded promising results for olive oil quality measurements, particularly for acidity, the peroxide index and alkyl and ethyl ester content. The results obtained indicate that this non-invasive technology can be used successfully by the olive oil sector to categorize olive oils, to detect potential fraud and to provide consumers with more reliable information. Although both sample presentation modes yielded comparable results, equations constructed with samples scanned using the spinning mode provided greater predictive capacity. PMID:29144417

The hard choice for alternative biofuels to diesel in Brazil.

PubMed

Carioca, J O B; Hiluy Filho, J J; Leal, M R L V; Macambira, F S

2009-01-01

This paper selects biofuel scenarios to substitute diesel in Brazil based on oil reserves increase, diesel imports, CO(2) emissions, crops agronomic yields, byproducts marketing and social impacts. This hard task still considers that agricultural practices in developing countries have large social impacts. Brazil presents high consumption of diesel oil in transport; low agronomic yield of traditional vegetable oil crops, which demand large cultivation areas contrasting with microalgae and palm oils which present high productivity. Concerning technologies, thermal cracking and transesterification of vegetable oils present a difficult economic situation related to vegetable oils price, food competition and glycerin market; BTL technology, meaning thermal gasification of biomass to liquids, faces problems related to low density of biomaterials and low viscosity of synthetic biodiesel produced. Biorefinery algal integrated systems and co-solvent technology to introduce up to 8% of ethanol into diesel seem to be feasible routes to reduce diesel consumption.

Effect of second cooling on the chemical components of essential oils from orange peel (Citrus sinensis).

PubMed

Chen, Yulong; Wu, Jijun; Xu, Yujuan; Fu, Manqing; Xiao, Gengsheng

2014-09-03

A second cooling was added to the oil collectors of an improved Clevenger-type apparatus (ICT) to investigate the thermal reaction of essential oils from orange peel compared to a traditional Clevenger-type apparatus (CT). The results demonstrated the yield rate of essential oil from ICT was significantly higher (p < 0.05) than that from CT. The major components of the essential oils consisted of monoterpenes, such as d-limonene, β-myrcene, β-pinene, γ-terpinene, α-pinene. Interestingly, ICT prevented the thermal reaction-the transformation of β-myrcene to β-thujene-and reduced the oxidation on α-pinene and β-pinene of the essential oil in comparison to CT. In addition, the yield rate of γ-terpinene can also be improved via ICT compared to CT. Thus, ICT is an effective improvement to traditional CT.

Torrefaction reduction of coke formation on catalysts used in esterification and cracking of biofuels from pyrolysed lignocellulosic feedstocks

DOEpatents

Kastner, James R; Mani, Sudhagar; Hilten, Roger; Das, Keshav C

2015-11-04

A bio-oil production process involving torrefaction pretreatment, catalytic esterification, pyrolysis, and secondary catalytic processing significantly reduces yields of reactor char, catalyst coke, and catalyst tar relative to the best-case conditions using non-torrefied feedstock. The reduction in coke as a result of torrefaction was 28.5% relative to the respective control for slow pyrolysis bio-oil upgrading. In fast pyrolysis bio-oil processing, the greatest reduction in coke was 34.9%. Torrefaction at 275.degree. C. reduced levels of acid products including acetic acid and formic acid in the bio-oil, which reduced catalyst coking and increased catalyst effectiveness and aromatic hydrocarbon yields in the upgraded oils. The process of bio-oil generation further comprises a catalytic esterification of acids and aldehydes to generate such as ethyl levulinate from lignified biomass feedstock.

Lactation performance of Holstein cows treated with 2 formulations of recombinant bovine somatotropin in a large commercial dairy herd in Brazil.

PubMed

de Morais, J P G; da S Cruz, A P; Minami, N S; Veronese, L P; Del Valle, T A; Aramini, J

2017-07-01

The objectives of this controlled study were to compare the effects of 2 different formulations of recombinant bovine somatotropin (rbST) on milk yield, milk composition (fat and protein), milk somatic cell count, and body condition score (BCS) among dairy cattle in a large commercial herd. Regulatory approved 500-mg zinc sesame oil base rbST (ZSO-rbST; Elanco Animal Health, Greenfield, IN) and vitamin E lecithin base rbST (VEL-rbST; LG Life Sciences, Seoul, South Korea) formulations were administered per the manufacturers' recommendations every 14 d over 17 injection cycles starting at 57 to 70 d of lactation (90 cows per rbST group). Control cows (n = 60) received no rbST. Somatotropin-treated animals (VEL-rbST and ZSO-rbST combined) had increased average milk yield and protein percentage and lower average BCS compared with control cows. For primiparous cows, average milk yield was 37.75 kg/d with the ZSO-rbST treatment and 35.72 kg/d with the VEL-rbST treatment. For multiparous cows, average milk yield was 40.13 kg/d with the ZSO-rbST treatment and 38.81 kg/d with the VEL-rbST treatment. There were no differences in milk fat percentage between VEL-rbST and ZSO-rbST treatments, but milk protein content was greater with VEL-rbST treatment than with ZSO-rbST treatment. Nonetheless, cows treated with ZSO-rbST yielded more kilograms of fat and protein per day than cows treated with VEL-rbST. No significant differences in BCS were found between both rbST treatment groups. The differential increase in milk yield between cows treated with ZSO-rbST and VEL-rbST was driven by rbST response differences both within the 14-d cycle and throughout the 17 injection cycles. The cows treated with VEL-rbST demonstrated a more variable 14-d milk yield response curve, with more pronounced valleys between injections compared with the ZSO-rbST formulation. In addition, only the ZSO-rbST treatment was effective in modifying the lactation persistency compared with control cows. Compared with the VEL-rbST formulation, the ZSO-rbST formulation yielded more kilograms of milk, fat, and protein with less milk variation throughout the seventeen 14-d lactation cycles for both primiparous and multiparous cows. The Authors. Published by the Federation of Animal Science Societies and Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

Microwave-assisted extraction of Nigella sativa L. essential oil and evaluation of its antioxidant activity.

PubMed

Abedi, Abdol-Samad; Rismanchi, Marjan; Shahdoostkhany, Mehrnoush; Mohammadi, Abdorreza; Mortazavian, Amir Mohammad

2017-11-01

It has been previously reported that the essential oil of Nigella sativa L. seeds and its major active component, thymoquinone (TQ), possess a broad variety of biological activities and therapeutic properties. In this work, microwave-assisted extraction (MAE) of the essential oil from Nigella sativa L. seeds and its antioxidant activity were studied. Response surface methodology based on central composite design was used to evaluate the effects of extraction time, irradiation power and moisture content on extraction yield and TQ content. Optimal parameters obtained by CCD and RSM were extraction time 30 min, irradiation power 450 W, and moisture content 50%. The extraction yield and TQ content of the essential oil were 0.33 and 20% under the optimum conditions, respectively. In contrast, extraction yield and TQ amount of oil obtained by hydrodistillation (HD) were 0.23 and 3.71%, respectively. The main constituents of the essential oil extracted by MAE and HD were p -cymene, TQ, α-thujene and longifolene, comprising more than 60% of total peak area. The antioxidant capacity of essential oils extracted by different methods were evaluated using 2,2-diphenyl-1-picrylhydrazyl and Ferric reducing antioxidant power assays, and compared with traditional antioxidants. The results showed that MAE method was a viable alternative to HD for the essential oil extraction from N. sativa seeds due to the excellent extraction efficiency, higher thymoquinone content, and stronger antioxidant activity.

Synthesis of fatty acid methyl ester from used vegetable cooking oil by solid reusable Mg 1-x Zn 1+x O2 catalyst.

PubMed

Olutoye, M A; Hameed, B H

2011-02-01

Fatty acid methyl ester was produced from used vegetable cooking oil using Mg(1-)(x) Zn(1+)(x)O(2) solid catalyst and the performance monitored in terms of ester content obtained. Used vegetable cooking oil was employed to reduce operation cost of biodiesel. The significant operating parameters which affect the overall yield of the process were studied. The highest ester content, 80%, was achieved with the catalyst during 4h 15 min reaction at 188°C with methanol to oil ratio of 9:1 and catalyst loading of 2.55 wt% oil. Also, transesterification of virgin oil gave higher yield with the heterogeneous catalyst and showed high selectivity towards ester production. The used vegetable cooking oil did not require any rigorous pretreatment. Catalyst stability was examined and there was no leaching of the active components, and its performance was as good at the fourth as at the first cycle. Copyright © 2010 Elsevier Ltd. All rights reserved.

Production of wax esters via microbial oil synthesis from food industry waste and by-product streams.

PubMed

Papadaki, Aikaterini; Mallouchos, Athanasios; Efthymiou, Maria-Nefeli; Gardeli, Chryssavgi; Kopsahelis, Nikolaos; Aguieiras, Erika C G; Freire, Denise M G; Papanikolaou, Seraphim; Koutinas, Apostolis A

2017-12-01

The production of wax esters using microbial oils was demonstrated in this study. Microbial oils produced from food waste and by-product streams by three oleaginous yeasts were converted into wax esters via enzymatic catalysis. Palm oil was initially used to evaluate the influence of temperature and enzyme activity on wax ester synthesis catalysed by Novozyme 435 and Lipozyme lipases using cetyl, oleyl and behenyl alcohols. The highest conversion yields (up to 79.6%) were achieved using 4U/g of Novozyme 435 at 70°C. Transesterification of microbial oils to behenyl and cetyl esters was achieved at conversion yields up to 87.3% and 69.1%, respectively. Novozyme 435 was efficiently reused for six and three cycles during palm esters and microbial esters synthesis, respectively. The physicochemical properties of microbial oil derived behenyl esters were comparable to natural waxes. Wax esters from microbial oils have potential applications in cosmetics, chemical and food industries. Copyright © 2017 Elsevier Ltd. All rights reserved.

Light response of sunflower and canola as affected by plant density, plant genotype and N fertilization.

PubMed

Soleymani, A

2017-08-01

Crop response to light is an important parameter determining crop growth. Three field (split plots) experiments were conducted to investigate the effects of plant density, plant genotype and N fertilization on the light absorption and light extinction of sunflower (Helianthus annuus L.) and canola (Brassica napus L.). A detailed set of plant growth, light absorption and crop yield and oil related parameters were determined. Light was measured at noon during the sunny days with clear sky. In experiment I, although the plant density (PD) of 14 resulted in the highest rate of sunflower light absorption (31.37%) and light extinction (0.756), the highest rate of grain yield and grain oil yield was resulted at PD12 at 3639 and 1457.9kg/ha, respectively; as well as by genotype SUP.A. In experiment II (canola), PD80 resulted in the highest rate of light absorption (13.13%), light extinction (0.63), grain yield (2189.4kg/ha) and grain oil yield (556.54kg/ha). This was also the case for Genotype H. In experiment III (canola), although N150 resulted in the highest rate of light absorption (10.74%) and light extinction (0.48), the highest rate of grain yield (3413.6kg/ha) and grain oil yield (891.86kg/ha) was resulted at N100 as well as by Genotype H401. Results indicate how light properties, crop growth and yield of sunflower and canola can be affected by plant and environmental parameters, which are also of practical use by farmers. Copyright © 2017 Elsevier B.V. All rights reserved.

Complementary blending of meadowfoam seed oil methyl esters with biodiesel prepared from soybean and waste cooking oils to enhance fuel properties

USDA-ARS?s Scientific Manuscript database

The complementary blending of meadowfoam seed oil methyl esters (MFME) with soybean and waste cooking oil methyl esters (SME and WCME) was investigated. MFME prepared from cold-pressed meadowfoam oil exhibited an exceptionally high induction period (IP) of 66.2 h whereas SME and WCME yielded conside...

Yield and oil composition of 38 basil (Ocimum basilicum L.) accessions grown in Mississippi.

PubMed

Zheljazkov, Valtcho D; Callahan, Amber; Cantrell, Charles L

2008-01-09

A field experiment was conducted to assess yield, oil content, and composition of 38 genotypes of sweet basil ( Ocimum basilicum L.). Overall, biomass yields were high and comparable to those reported in the literature. However, basil genotypes differed significantly with respect to oil content and composition. Oil content of the tested accessions varied from 0.07% to 1.92% in dry herbage. On the basis of the oil composition, basil accessions were divided into seven groups: (1) high-linalool chemotype [19-73% (-)-linalool], (2) linalool-eugenol chemotype [six chemotypes with 28-66% (-)-linalool and 5-29% eugenol], (3) methyl chavicol chemotype [six accessions with 20-72% methyl chavicol and no (-)-linalool], (4) methyl chavicol-linalool chemotype [six accessions with 8-29% methyl chavicol and 8-53% (-)-linalool], (5) methyl eugenol-linalool chemotype [two accessions with 37% and 91% methyl eugenol and 60% and 15% (-)-linalool], (6) methyl cinnamate-linalool chemotype [one accession with 9.7% methyl cinnamate and 31% (-)-linalool], and (7) bergamotene chemotype [one accession with bergamotene as major constituent, 5% eucalyptol, and <1% (-)-linalool]. Our results demonstrated that basil could be a viable essential oil crop in Mississippi. The availability of various chemotypes offers the opportunity for production of basil to meet the market requirements of specific basil oils or individual compounds such as (-)-linalool, eugenol, methyl chavicol, methyl cinnamate, or methyl eugenol.

Hydrotreatment of bio-oil distillates produced from pyrolysis and hydrothermal liquefaction of duckweed: A comparison study.

PubMed

Wang, Feng; Tian, Ye; Zhang, Cai-Cai; Xu, Yu-Ping; Duan, Pei-Gao

2018-09-15

A comprehensive comparison of hydrothermal liquefaction (HTL) to the pyrolysis of duckweed was conducted to determine the yields and components of the crude bio-oils and their distillates. The upgrading behaviors of the distillates were thoroughly investigated with the use of used engine oil as a solvent. With all other variables fixed, HTL produced crude bio-oil with a lower H/C ratio (1.28 ± 0.03) than pyrolysis did (1.45 ± 0.04). However, its distillates had a higher H/C ratio (1.60 ± 0.05) and total yield (66.1 ± 2.0 wt%) than pyrolysis (1.46 ± 0.04 and 47.2 ± 1.4 wt%, respectively). Phenolics and nitrogenous heterocycles constituted relatively major proportions of the two crude bio-oils and most of their distillates. Obvious differences in molecular composition between the two crude bio-oils and their distillates were ascribed to the distinct impacts of HTL and pyrolysis and were affected by the distillate temperature. Co-hydrotreating with used engine oil (UEO) provided the upgraded bio-oils much higher H/C ratios (~1.78 ± 0.05) and higher heating values (~45.5 ± 1.4 MJ·kg -1 ), as well as much lower contents of N, O and S compared to their initial distillates. Aromatics and alkanes constituted a large proportion in most of upgraded bio-oils. N removal from the pyrolysis distillates was easier than from the HTL distillates. Distinct differences in yields and molecular compositions for the upgraded bio-oils were also attributed to the different influences associated with the two conversion routes. Copyright © 2018 Elsevier B.V. All rights reserved.

Application of different fertilizers on morphological traits of dill (Anethum graveolens L.).

PubMed

Nejatzadeh-Barandozi, Fatemeh; Gholami-Borujeni, Fathollah

2014-12-01

The aim of this study was to evaluate the effects of nitroxin biofertilizer and chemical fertilizer on the growth, yield, and essential oil composition of dill. The experiment was conducted under field condition in randomized complete block design with three replications and two factors. The first factor was the concentrations of nitroxin biofertilizer (0%, 50%, and 100%) of the recommended amount (1 l of biological fertilizer for 30 kg of seed). The second factor was the following chemical fertilizer treatments: no fertilizer (control) and 50 and 100 kg ha(-1) urea along with 300 kg ha(-1) ammonium phosphate. Different characteristics such as plant height, number of umbel per plant, number of umbellet per umbel, number of grain per umbellet, 1,000 seed weight, grain yield, biological yield, and oil percentage were recorded. According to the results, the highest height, biological yield, and grain yield components (except harvest index) were obtained on biological fertilizer. The results showed the highest essential oil content detected in biological fertilizer and chemical fertilizer. Identification of essential oil composition showed that the content of carvone increased with the application of biofertilizers and chemical fertilizers. The results indicated that the application of biofertilizers enhanced yield and other plant criteria in this plant. Generally, it seems that the use of biofertilizers or combinations of biofertilizer and chemical fertilizer could improve dill performance in addition to reduction of environmental pollution.

Extraction methods of Amaranthus sp. grain oil isolation.

PubMed

Krulj, Jelena; Brlek, Tea; Pezo, Lato; Brkljača, Jovana; Popović, Sanja; Zeković, Zoran; Bodroža Solarov, Marija

2016-08-01

Amaranthus sp. is a fast-growing crop with well-known beneficial nutritional values (rich in protein, fat, dietary fiber, ash, and minerals, especially calcium and sodium, and containing a higher amount of lysine than conventional cereals). Amaranthus sp. is an underexploited plant source of squalene, a compound of high importance in the food, cosmetic and pharmaceutical industries. This paper has examined the effects of the different extraction methods (Soxhlet, supercritical fluid and accelerated solvent extraction) on the oil and squalene yield of three genotypes of Amaranthus sp. grain. The highest yield of the extracted oil (78.1 g kg(-1) ) and squalene (4.7 g kg(-1) ) in grain was obtained by accelerated solvent extraction (ASE) in genotype 16. Post hoc Tukey's HSD test at 95% confidence limit showed significant differences between observed samples. Principal component analysis (PCA) and cluster analysis (CA) were used for assessing the effect of different genotypes and extraction methods on oil and squalene yield, and also the fatty acid composition profile. Using coupled PCA and CA of observed samples, possible directions for improving the quality of product can be realized. The results of this study indicate that it is very important to choose both the right genotype and the right method of extraction for optimal oil and squalene yield. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

Catalytic pyrolysis of car tire waste using expanded perlite.

PubMed

Kar, Y

2011-08-01

In this study, the non-catalytic and catalytic pyrolysis experiments were conducted on the sample of tire waste using expanded perlite as an additive material to determine especially the effect of temperature and catalyst-to-tire ratio on the products yields and the compositions and qualities of pyrolytic oils (NCPO and CPO). Non-catalytic studies, which were carried out under the certain conditions (a nitrogen flow of 100mL/min and a heating rate of 10°C/min), showed that the highest yield of pyrolytic oil (NCPO) was 60.02wt.% at 425°C. Then, the catalytic pyrolysis studies were carried out at catalyst-to-tire ratio range of 0.05-0.25 and the highest catalytic pyrolytic oil (CPO) yield was 65.11wt.% at the ratio of 0.10 with the yield increase of 8.48wt.% compared with the non-catalytic pyrolysis. Lastly, the pyrolytic oils were characterized with applying a various techniques such as elemental analyses and various chromatographic and spectroscopic techniques (GC-MS, (1)H NMR, FT-IR, etc.). The characterization results revealed that the pyrolytic oils which were complex mixtures of C(5)-C(15) organic compounds (predominantly aromatic compounds) and also the CPO compared to the NCPO was more similar to conventional fuels in view of the certain fuel properties. Copyright © 2011 Elsevier Ltd. All rights reserved.

Chemicals derived from pyrolysis bio-oils as antioxidants in fuels and lubricants

USDA-ARS?s Scientific Manuscript database

Softwood and hardwood lignins and hardwood were pyrolyzed to produce bio-oils to produce lignin-derived bio-oils of which phenols were the major component. These bio-oils were extracted with alkali to yield a range of lignin-related phenols having molecular weights (MWs) from 110 to 344. When tested...

Oils from wild, micropropagated plants, calli, and suspended cells of Euphorbia characias L.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fernandes-Ferreira, M.; Pais, M.S.S.; Novais, J.M.

1991-12-31

Micropropagated Euphorbia characias plants gave higher yields of crude oil than did wild ones. Leaves of either wild and micropropagated plants contained more oil than did stems. Triterpenols, hydrocarbons, and free and esterified fatty acids are components of the crude oil produced by stems, young and mature leaves of wild and micropropagated E. characias plants, as well as by calli and suspended cells. With the exception of the free fatty acids fraction, all crude oil fractions were higher in micropropagated plants than in the wild ones. The crude oil content of leaves of either wild or micropropagated plants was highermore » than that of stems. However the triterpenols yields were higher in stems than in leaves, both in wild and micropropagated plants. The composition of the triterpenol fraction of the crude oil obtained from calli and suspended cells is quite different from that produced by any in vivo parent plant organ studied. Free fatty acids constitute the main fraction of the crude oil obtained from calli and suspended cells.« less

Catalytic cracking of Mayan gas oil and selected hydrotreated products: Topical report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wells, J.W.; Zagula, E.J.; Brinkman, D.W.

1988-01-01

The catalytic cracking of a Mayan vacuum gas oil and the products from mild, moderate, and severe hydrotreating of this gas oil was evaluated over a low-metal equilibrium catalyst in a microconfined bed unit (MCBU). Results obtained with the Mayan feedstocks are compared with those of an earlier study conducted with similar feedstocks obtained from a Wilmington (CA) crude oil. Two levels of catalytic cracking severity were used in the evaluation. Performance and product analysis showed that hydrotreating improves the yields obtained from catalytic cracking and the quality of the resultant products. In contrast to results obtained with the Wilmingtonmore » feedstocks, conversion and gasoline yield do not improve with severity of the hydrotreating of the Mayan vacuum gas oils. The insensitivity of the cracking performance to hydrotreating severity may reflect the more facile removal of polar compounds (heteroatom compounds) on hydrotreating of the Mayan gas oil in comparison to the Wilmington. Sulfur and nitrogen contents of the liquid products (gasoline, light cycle oil, heavy cycle oil) decreased as the severity of the feed hydrotreating increased. 7 refs., 12 figs., 15 tabs.« less

Microwave-assisted extraction of silkworm pupal oil and evaluation of its fatty acid composition, physicochemical properties and antioxidant activities.

PubMed

Hu, Bin; Li, Cheng; Zhang, Zhiqing; Zhao, Qing; Zhu, Yadong; Su, Zhao; Chen, Yizi

2017-09-15

Microwave-assisted extraction (MAE) of oil from silkworm pupae was firstly performed in the present research. The response surface methodology was applied to optimize the parameters for MAE. The yield of oil by MAE was 30.16% under optimal conditions of a mixed solvent consisting of ethanol and n-hexane (1:1, v/v), microwave power (360W), liquid to solid ratio (7.5/1mL/g), microwave time (29min). Moreover, oil extracted by MAE was quantitatively (yield) and qualitatively (fatty acid profile) similar to those obtained using Soxhlet extraction (SE), but oil extracted by MAE exhibited favourable physicochemical properties and oxidation stability. Additionally, oil extracted by MAE had a higher content of total phenolic, and it showed stronger antioxidant activities. Scanning electron microscopy revealed that microwave technique efficiently promoted the release of oil by breaking down the cell structure of silkworm pupae. Therefore, MAE can be an effective method for the silkworm pupal oil extraction. Copyright © 2017 Elsevier Ltd. All rights reserved.

Synthesis of fatty acid methyl ester from the transesterification of high- and low-acid-content crude palm oil (Elaeis guineensis) and karanj oil (Pongamia pinnata) over a calcium-lanthanum-aluminum mixed-oxides catalyst.

PubMed

Syamsuddin, Y; Murat, M N; Hameed, B H

2016-08-01

The synthesis of fatty acid methyl ester (FAME) from the high- and low-acid-content feedstock of crude palm oil (CPO) and karanj oil (KO) was conducted over CaO-La2O3-Al2O3 mixed-oxide catalyst. Various reaction parameters were investigated using a batch reactor to identify the best reaction condition that results in the highest FAME yield for each type of oil. The transesterification of CPO resulted in a 97.81% FAME yield with the process conditions of 170°C reaction temperature, 15:1 DMC-to-CPO molar ratio, 180min reaction time, and 10wt.% catalyst loading. The transesterification of KO resulted in a 96.77% FAME yield with the conditions of 150°C reaction temperature, 9:1 DMC-to-KO molar ratio, 180min reaction time, and 5wt.% catalyst loading. The properties of both products met the ASTM D6751 and EN 14214 standard requirements. The above results showed that the CaO-La2O3-Al2O3 mixed-oxide catalyst was suitable for high- and low-acid-content vegetable oil. Copyright © 2016 Elsevier Ltd. All rights reserved.

Colored shade nets induced changes in growth, anatomy and essential oil of Pogostemon cablin.

PubMed

Ribeiro, Aurislaine S; Ribeiro, Mariana S; Bertolucci, Suzan K V; Bittencourt, Wanderley J M; Carvalho, Alexandre A DE; Tostes, Wesley N; Alves, Eduardo; Pinto, José E B P

2018-04-16

The purpose of this investigation was to determine the influence of colored shade nets on the growth, anatomy and essential oil content, yield and chemical composition of Pogostemon cablin. The plants were cultivated under full sunlight, black, blue and red nets. The harvesting was performed 5 months after planting and it was followed by the analysis of plant growth parameters, leaf anatomy, essential oil content, yield and chemical composition. The plants grown under red net have produced more leaf, shoot, total dry weight and leaf area. Plants cultivated under colored nets showed differences in morphological features. Plants maintained under red net had a higher leaf blade thickness and polar and equatorial diameter of the stomata ratio. Additionally, higher yield of essential oil in the leaves was observed under red and blue colored shade net. The essential oil of the plants grown under red net showed the highest relative percentage of patchoulol (66.84%). Therefore, it is possible using colored shade nets to manipulate P. cablin growth, as well as its essential oil production with several chemical compositions. The analyses of principal components allowed observing that pogostol has negative correlation with α-guaiene and α-bulnesene. There was difference in total dry weight and patchoulol content when the patchouli is cultured under the red colored shade nets.

Comparative Effect of Dietary Soybean Oil, Fish Oil, and Coconut Oil on Performance, Egg Quality and Some Blood Parameters in Laying Hens.

PubMed

Dong, X F; Liu, S; Tong, J M

2018-04-14

Two hundred and sixteen 28-wk-old Hy-line laying hens were randomly distributed to three dietary treatments and fed 1of 3 diets containing 8% soybean oil, fish oil, or coconut oil from 28 to 47 wk of age to investigate comparative effect of dietary soybean oil, fish oil, and coconut oil on the performance, egg quality and blood malondialdehyde (MDA), aspartate transaminase (AST) and uric acid (UA). Hens fed fish oil showed poor performance compared with soybean oil or coconut oil, and especially egg weight throughout the trial was significantly and consistently decreased (P < 0.05) due to dietary fish oil. Unexpectedly, shell reflectivity throughout the majority of the trial was consistently and significantly higher (P < 0.05) when hens fed fish oil than that when fed soybean oil or coconut oil. Dietary treatments affected (P < 0.05) shell shape at 4 of 8 time points tested. Average shell shape in fish oil treatment was higher (P < 0.05) than that of coconut oil group. Albumen height, Haugh unit and yolk color were influenced by dietary treatments only at 1 or 2 time points. However, average albumen height and Haugh unit in fish oil treatment were higher (P < 0.05) than that of soybean oil or coconut oil treatments and average yolk color in coconut oil treatment was higher (P < 0.05) than that of soybean oil group. Serum MDA, AST and UA concentrations were increased (P < 0.05) by fish oil during the majority of the first 2 mo of the trial. These data suggested that the inclusion of fish oil into feed may reduce the performance of laying hens, especially the egg weight, decrease the intensity of egg brown color and increase blood MDA, AST and UA levels compared with soybean oil or coconut oil. As a result, hens fed fish oil may lay smaller, longer and lighter-brown eggs whereas those fed coconut oil produce blunter and darker-brown eggs relative to soybean oil.

Reductive Catalytic Fractionation of Corn Stover Lignin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anderson, Eric M.; Katahira, Rui; Reed, Michelle

Reductive catalytic fractionation (RCF) has emerged as an effective biomass pretreatment strategy to depolymerize lignin into tractable fragments in high yields. We investigate the RCF of corn stover, a highly abundant herbaceous feedstock, using carbon-supported Ru and Ni catalysts at 200 and 250 degrees C in methanol and, in the presence or absence of an acid cocatalyst (H3PO4 or an acidified carbon support). Three key performance variables were studied: (1) the effectiveness of lignin extraction as measured by the yield of lignin oil, (2) the yield of monomers in the lignin oil, and (3) the carbohydrate retention in the residualmore » solids after RCF. The monomers included methyl coumarate/ferulate, propyl guaiacol/syringol, and ethyl guaiacol/syringol. The Ru and Ni catalysts performed similarly in terms of product distribution and monomer yields. The monomer yields increased monotonically as a function of time for both temperatures. At 6 h, monomer yields of 27.2 and 28.3% were obtained at 250 and 200 degrees C, respectively, with Ni/C. The addition of an acid cocatalysts to the Ni/C system increased monomer yields to 32% for acidified carbon and 38% for phosphoric acid at 200 degrees C. The monomer product distribution was dominated by methyl coumarate regardless of the use of the acid cocatalysts. The use of phosphoric acid at 200 degrees C or the high temperature condition without acid resulted in complete lignin extraction and partial sugar solubilization (up to 50%) thereby generating lignin oil yields that exceeded the theoretical limit. In contrast, using either Ni/C or Ni on acidified carbon at 200 degrees C resulted in moderate lignin oil yields of ca. 55%, with sugar retention values >90%. Notably, these sugars were amenable to enzymatic digestion, reaching conversions >90% at 96 h. Characterization studies on the lignin oils using two-dimensional heteronuclear single quantum coherence nuclear magnetic resonance and gel permeation chromatrography revealed that soluble oligomers are formed via solvolysis, followed by further fragmentation on the catalyst surface via hydrogenolysis. Overall, the results show that clear trade-offs exist between the levels of lignin extraction, monomer yields, and carbohydrate retention in the residual solids for different RCF conditions of corn stover.« less

[Contributions to the chemical study of the essential oil isolated from coriander fruits (Sandra cultivar)].

PubMed

Trifan, Adriana; Aprotosoaie, Ana Clara; Spac, A; Hăncianu, Monica; Miron, Anca; Stănescu, Ursula

2011-01-01

Coriandrum sativum L. (Apiaceae) is a well known herb, native to the Mediterranean region, also intensively cultivated in Romania. The essential oil obtained from Coriandri fructus posseses antimicrobial, antioxidant and anxiolytic effects. Many parameters such as genetic and climatic factors or agronomical practices can influence the yield and composition of the volatile fraction. Plant density is an important factor for the microenvironment in coriander field. In order to study the effect of planting density on the yield of the essential oil and its composition, a bifactorial experiment was carried out on coriander plants (Sandra cultivar). The experiment was performed with three plant densities on the row (0, 15 and 20 cm); the distance between plant rows was 12.5, 25 and 50 cm, respectively. So, it resulted nine experimental variants. The essential oils obtained by hydrodistillation from fruits have been characterized using gas chromatography and mass spectroscopy analysis (GC-MS). The highest yield (7.9866 kg/ha) was obtained for the plants spaced at 20 cm in between and 25 cm row spacing. The highest content of monoterpene alcohols (50.96%) was obtained with 25 cm row spacing and plant spaced at 0 cm on the row. The main components in all oils were monoterpene alcohols (40.75% - 50.96%) and monoterpenes (32.43-38.44%). The essential oil of coriander fruits (Sandra cultivar) does not meet the requirements of the European Pharmacopoeia, especially concerning the content in linalool. Nevertheless, the high content in monoterpene alcohols and monoterpenes recommends the use of the essential oil as immunomodulatory, analgesic and antiinflammatory agent in rheumatology and also as an antibacterial and antiviral agent. Consequently, the changes in yield and composition of the essential oil of Sandra coriander should be assesed during several periods of vegetation in order to conclude on its pharmaceutical quality.

Effect of catalyst additives on the production of biofuels from palm oil cracking in a transport riser reactor.

PubMed

Chew, Thiam Leng; Bhatia, Subhash

2009-05-01

Catalytic cracking of crude palm oil (CPO) and used palm oil (UPO) were studied in a transport riser reactor for the production of biofuels at a reaction temperature of 450 degrees C, with residence time of 20s and catalyst-to-oil ratio (CTO) of 5 gg(-1). The effect of HZSM-5 (different Si/Al ratios), beta zeolite, SBA-15 and AlSBA-15 were studied as physically mixed additives with cracking catalyst Rare earth-Y (REY). REY catalyst alone gave 75.8 wt% conversion with 34.5 wt% of gasoline fraction yield using CPO, whereas with UPO, the conversion was 70.9 wt% with gasoline fraction yield of 33.0 wt%. HZSM-5, beta zeolite, SBA-15 and AlSBA-15 as additives with REY increased the conversion and the yield of organic liquid product. The transport riser reactor can be used for the continuous production of biofuels from cracking of CPO and UPO over REY catalyst.

Influence of the reaction conditions on the enzyme catalyzed transesterification of castor oil: A possible step in biodiesel production.

PubMed

Andrade, Thalles A; Errico, Massimiliano; Christensen, Knud V

2017-11-01

The identification of the influence of the reaction parameters is of paramount importance when defining a process design. In this work, non-edible castor oil was reacted with methanol to produce a possible component for biodiesel blends, using liquid enzymes as the catalyst. Temperature, alcohol-to-oil molar ratio, enzyme and added water contents were the reaction parameters evaluated in the transesterification reactions. The optimal conditions, giving the optimal final FAME yield and FFA content in the methyl ester-phase was identified. At 35°C, 6.0 methanol-to-oil molar ratio, 5wt% of enzyme and 5wt% of water contents, 94% of FAME yield and 6.1% of FFA in the final composition were obtained. The investigation was completed with the analysis of the component profiles, showing that at least 8h are necessary to reach a satisfactory FAME yield together with a minor FFA content. Copyright © 2017 Elsevier Ltd. All rights reserved.

Production of biodiesel from Jatropha curcas L. oil catalyzed by SO₄²⁻/ZrO₂ catalyst: effect of interaction between process variables.

PubMed

Yee, Kian Fei; Lee, Keat Teong; Ceccato, Riccardo; Abdullah, Ahmad Zuhairi

2011-03-01

This study reports the conversion of Jatrophacurcas L. oil to biodiesel catalyzed by sulfated zirconia loaded on alumina catalyst using response surface methodology (RSM), specifically to study the effect of interaction between process variables on the yield of biodiesel. The transesterification process variables studied were reaction temperature, reaction duration, molar ratio of methanol to oil and catalyst loading. Results from this study revealed that individual as well as interaction between variables significantly affect the yield of biodiesel. With this information, it was found that 4h of reaction at 150°C, methanol to oil molar ratio of 9.88 mol/mol and 7.61 wt.% for catalyst loading gave an optimum biodiesel yield of 90.32 wt.%. The fuel properties of Jatropha biodiesel were characterized and it indeed met the specification for biodiesel according to ASTM D6751. Copyright © 2010 Elsevier Ltd. All rights reserved.

Isolation and characterization of hydrophobic compounds from carbohydrate matrix of Pistacia atlantica.

PubMed

Samavati, Vahid; Adeli, Mostafa

2014-01-30

The present work is focused on the optimization of hydrophobic compounds extraction process from the carbohydrate matrix of Iranian Pistacia atlantica seed at laboratory level using ultrasonic-assisted extraction. Response surface methodology (RSM) was used to optimize oil seed extraction yield. Independent variables were extraction temperature (30, 45, 60, 75 and 90°C), extraction time (10, 15, 20, 25, 30 and 35 min) and power of ultrasonic (20, 40, 60, 80 and 100 W). A second order polynomial equation was used to express the oil extraction yield as a function of independent variables. The responses and variables were fitted well to each other by multiple regressions. The optimum extraction conditions were as follows: extraction temperature of 75°C, extraction time of 25 min, and power of ultrasonic of 80 W. A comparison between seed oil composition extracted by ultrasonic waves under the optimum operating conditions determined by RSM for oil yield and by organic solvent was reported. Copyright © 2013 Elsevier Ltd. All rights reserved.

Investigation of waste biomass co-pyrolysis with petroleum sludge using a response surface methodology.

PubMed

Hu, Guangji; Li, Jianbing; Zhang, Xinying; Li, Yubao

2017-05-01

The treatment of waste biomass (sawdust) through co-pyrolysis with refinery oily sludge was carried out in a fixed-bed reactor. Response surface method was applied to evaluate the main and interaction effects of three experimental factors (sawdust percentage in feedstock, temperature, and heating rate) on pyrolysis oil and char yields. It was found that the oil and char yields increased with sawdust percentage in feedstock. The interaction between heating rate and sawdust percentage as well as between heating rate and temperature was significant on the pyrolysis oil yield. The higher heating value of oil originated from sawdust during co-pyrolysis at a sawdust/oily sludge ratio of 3:1 increased by 5 MJ/kg as compared to that during sawdust pyrolysis alone, indicating a synergistic effect of co-pyrolysis. As a result, petroleum sludge can be used as an effective additive in the pyrolysis of waste biomass for improving its energy recovery. Copyright © 2017 Elsevier Ltd. All rights reserved.

Effect of Subcritical Fluid Extraction on the High Quality of Headspace Oil from Jasminum sambac (L.) Aiton.

PubMed

Ye, Qiuping; Jin, Xinyi; Wei, Shiqin; Zheng, Gongyu; Li, Xinlei

2016-05-01

Subcritical fluid extraction (SFE), as a novel method, was applied to investigate the yield, quality, and sensory evaluation of headspace oil from Jasminum sambac (L.) Aiton in comparison with petroleum ether extraction (PEE). The results indicated that the yield of the headspace oil using SFE was significantly higher (P < 0.05) than when using PEE. SFE contributed to obtaining alcohols and ethers, prevented the thermal reaction of terpenes, and reduced α-caryophyllene and β-caryophyllene in the headspace oil. The contents of linalool (21.90%) and benzyl acetate (16.31%) were higher via SFE than PEE. In addition, the sensory evaluation of SFE was superior to PEE, indicating a fresh, jasmine-like odor and green-yellow color. Thus, SFE is an improved method for obtaining natural headspace oil from jasmine flowers.

Catalytic cracking of the top phase fraction of bio-oil into upgraded liquid oil

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sunarno; Chemical Engineering Department, Gadjah Mada University, Jalan Grafika No. 2 Bulaksumur,Yogyakarta 55281; Rochmadi,

The energy consumption is increasing, while oil reserves as a primary energy resource are decreasing, so that is the reason seeking alternative energy source is inevitable. Biomass especially oil palm empty fruit bunches (EFB) which is abundant in Indonesia can be processed into bio-oil by pyrolysis process. The potential for direct substitution of bio-oil for petroleum may be limited due to the high viscosity, high oxygen content, low heating value, and corrosiveness. Consequently, upgrading of the bio-oil before use is inevitable to give a wider variety of applications of its liquid product. Furthermore, upgrading process to improve the quality ofmore » bio-oil by reduction of oxygenates involves process such as catalytic cracking. The objective of this research is to study the effect of operation temperature on yield and composition of upgraded liquid oil and to determine physical properties. Bio-oil derived from EFB was upgraded through catalytic cracking using series tubular reactor under atmospheric pressure on a silica-alumina catalyst. Results show that increasing temperature from 450 to 600 °C, resulting in decreasing of upgraded liquid oil (ULO) yield, decreasing viscosity and density of ULO, but increasing in calorimetric value of ULO. The increasing temperature of cracking also will increase the concentration of gasoline and kerosene in ULO.« less

Feasibility of observing small differences in friction mean effective pressure between different lubricating oil formations using small, single-cylinder motored engine rig

DOE PAGES

Rohr, William F.; Nguyen, Ke; Bunting, Bruce G.; ...

2015-09-01

Here, the feasibility of using a motored single-cylinder 517 cc diesel engine to observe small frictional differences between oil formulations is investigated. Friction mean effective pressure (FMEP) is measured and compared for an SAE 10W-30 and an SAE 5W-20 oil in three stages of production: base oil, commercial oil without a friction and wear reducing additive, and fully formulated commercial oil. In addition, a commercial SAE 5W-30 engine oil is investigated. Friction mean effective pressure is plotted versus oil dynamic viscosity to compare the lubricant FMEP at a given viscosity. Linear regressions and average friction mean effective pressure are usedmore » as a secondary means of comparing FMEP for the various oil formulations. Differences between the oils are observed with the base oil having higher friction at a given viscosity but a lower average FMEP due to the temperature distribution of the test and lower viscosities reached by the base oil. The commercial oil is shown to have both a higher FMEP at a given viscosity and a higher average FMEP than the commercial oil without a friction and wear reducing additive. The increase in friction for the oil without a friction and wear reduction additive indicates that the operational regime of the engine may be out of the bounds of the optimal regime for the additive or that the additive is more optimized for wear reduction. Results show that it is feasible to observe small differences in FMEP between lubricating oil formulations using a small, single-cylinder motored engine.« less

Feasibility of observing small differences in friction mean effective pressure between different lubricating oil formations using small, single-cylinder motored engine rig

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rohr, William F.; Nguyen, Ke; Bunting, Bruce G.

Here, the feasibility of using a motored single-cylinder 517 cc diesel engine to observe small frictional differences between oil formulations is investigated. Friction mean effective pressure (FMEP) is measured and compared for an SAE 10W-30 and an SAE 5W-20 oil in three stages of production: base oil, commercial oil without a friction and wear reducing additive, and fully formulated commercial oil. In addition, a commercial SAE 5W-30 engine oil is investigated. Friction mean effective pressure is plotted versus oil dynamic viscosity to compare the lubricant FMEP at a given viscosity. Linear regressions and average friction mean effective pressure are usedmore » as a secondary means of comparing FMEP for the various oil formulations. Differences between the oils are observed with the base oil having higher friction at a given viscosity but a lower average FMEP due to the temperature distribution of the test and lower viscosities reached by the base oil. The commercial oil is shown to have both a higher FMEP at a given viscosity and a higher average FMEP than the commercial oil without a friction and wear reducing additive. The increase in friction for the oil without a friction and wear reduction additive indicates that the operational regime of the engine may be out of the bounds of the optimal regime for the additive or that the additive is more optimized for wear reduction. Results show that it is feasible to observe small differences in FMEP between lubricating oil formulations using a small, single-cylinder motored engine.« less

Distillation time modifies essential oil yield, composition, and antioxidant capacity of fennel (Foeniculum vulgare Mill).

PubMed

Zheljazkov, Valtcho D; Horgan, Thomas; Astatkie, Tess; Schlegel, Vicki

2013-01-01

Fennel (Foeniculum vulgare Mill) is an essential oil crop grown worldwide for production of essential oil, as medicinal or as culinary herb. The essential oil is extracted via steam distillation either from the whole aboveground biomass (herb) or from fennel fruits (seed). The hypothesis of this study was that distillation time (DT) can modify fennel oil yield, composition, and antioxidant capacity of the oil. Therefore, the objective of this study was to evaluate the effect of eight DT (1.25, 2.5, 5, 10, 20, 40, 80, and 160 min) on fennel herb essential oil. Fennel essential oil yield (content) reached a maximum of 0.68% at 160 min DT. The concentration of trans-anethole (32.6-59.4% range in the oil) was low at 1.25 min DT, and increased with an increase of the DT. Alpha-phelandrene (0.9-10.5% range) was the lowest at 1.25 min DT and higher at 10, 80, and 160 min DT. Alpha-pinene (7.1-12.4% range) and beta-pinene (0.95-1.64% range) were higher in the shortest DT and the lowest at 80 min DT. Myrcene (0.93-1.95% range), delta-3-carene (2.1-3.7% range), cis-ocimene (0-0.23% range), and gamma-terpinene (0.22-2.67% range) were the lowest at 1.25 min DT and the highest at 160 min DT. In contrast, the concentrations of paracymene (0.68-5.97% range), fenchone (9.8-22.7% range), camphor (0.21-0.51% range), and cis-anethole (0.14-4.66% range) were highest at shorter DT (1.25-5 min DT) and the lowest at the longer DT (80-160 min DT). Fennel oils from the 20 and 160 min DT had higher antioxidant capacity than the fennel oil obtained at 1.25 min DT. DT can be used to obtain fennel essential oil with differential composition. DT must be reported when reporting essential oil content and composition of fennel essential oil. The results from this study may be used to compare reports in which different DT to extract essential oil from fennel biomass were used.

Comparison of Moringa Oleifera seeds oil characterization produced chemically and mechanically

NASA Astrophysics Data System (ADS)

Eman, N. A.; Muhamad, K. N. S.

2016-06-01

It is established that virtually every part of the Moringa oleifera tree (leaves, stem, bark, root, flowers, seeds, and seeds oil) are beneficial in some way with great benefits to human being. The tree is rich in proteins, vitamins, minerals. All Moringa oleifera food products have a very high nutritional value. They are eaten directly as food, as supplements, and as seasonings as well as fodder for animals. The purpose of this research is to investigate the effect of seeds particle size on oil extraction using chemical method (solvent extraction). Also, to compare Moringa oleifera seeds oil properties which are produced chemically (solvent extraction) and mechanically (mechanical press). The Moringa oleifera seeds were grinded, sieved, and the oil was extracted using soxhlet extraction technique with n-Hexane using three different size of sample (2mm, 1mm, and 500μm). The average oil yield was 36.1%, 40.80%, and 41.5% for 2mm, 1mm, and 500μm particle size, respectively. The properties of Moringa oleifera seeds oil were: density of 873 kg/m3, and 880 kg/m3, kinematic viscosity of 42.2mm2/s and 9.12mm2/s for the mechanical and chemical method, respectively. pH, cloud point and pour point were same for oil produced with both methods which is 6, 18°C and 12°C, respectively. For the fatty acids, the oleic acid is present with high percentage of 75.39%, and 73.60% from chemical and mechanical method, respectively. Other fatty acids are present as well in both samples which are (Gadoleic acid, Behenic acid, Palmitic acid) which are with lower percentage of 2.54%, 5.83%, and 5.73%, respectively in chemical method oil, while they present as 2.40%, 6.73%, and 6.04%, respectively in mechanical method oil. In conclusion, the results showed that both methods can produce oil with high quality. Moringa oleifera seeds oil appear to be an acceptable good source for oil rich in oleic acid which is equal to olive oil quality, that can be consumed in Malaysia where the olive oil is imported with high prices. In the same time cultivation of Moringa oleifera tree is considered to be a new source of income for the country and give more job opportunities.

Water and Temperature Stresses Impact Canola (Brassica napus L.) Fatty Acid, Protein, and Yield over Nitrogen and Sulfur.

PubMed

Hammac, W Ashley; Maaz, Tai M; Koenig, Richard T; Burke, Ian C; Pan, William L

2017-12-06

Interactive effects of weather and soil nutrient status often control crop productivity. An experiment was conducted to determine effects of nitrogen (N) and sulfur (S) fertilizer rate, soil water, and atmospheric temperature on canola (Brassica napus L.) fatty acid (FA), total oil, protein, and grain yield. Nitrogen and sulfur were assessed in a 4-yr study with two locations, five N rates (0, 45, 90, 135, and 180 kg ha -1 ), and two S rates (0 and 17 kg ha -1 ). Water and temperature were assessed using variability across 12 site-years of dryland canola production. Effects of N and S were inconsistent. Unsaturated FA, oleic acid, grain oil, protein, and theoretical maximum grain yield were highly related to water and temperature variability across the site-years. A nonlinear model identified water and temperature conditions that enabled production of maximum unsaturated FA content, oleic acid content, total oil, protein, and theoretical maximum grain yield. Water and temperature variability played a larger role than soil nutrient status on canola grain constituents and yield.

Interfacial spreading effects on one-dimensional organic liquid imbibition in water-wetted porous media

NASA Astrophysics Data System (ADS)

McBride, J. F.; Simmons, C. S.; Cary, J. W.

1992-10-01

The spreading coefficient, Csp, determines whether an organic immiscible liquid, OIL, will form a lens ( Csp < 0) or will spread spontaneously ( Csp > 0) on a water surface. An OIL that forms a lens does not perfectly wet the water surface and therefore has a contact angle greater than 0°. The one-dimensional rate at which an OIL spreads spontaneously on a water surface is proportional to the square root of Csp. Of the OIL's that pose a contaminant threat to the subsurface, the majority has a non-zero Csp. To test the influence of such interfacial spreading phenomena on OIL infiltration in a pristine vadose zone, upward OIL and water imbibition infiltration experiments were performed in glass-bead columns, moistened with water, by using OIL's with different Csp. An analytical model for saturated liquid front rise was used to inversely estimate the effective capillary pressure head at the front and the average liquid conductivity. A nonspreading OIL ( Csp ≪ 0) exhibited a reduced capillary pressure head in the water-wetted glass beads. A spontaneously spreading OIL ( Csp ≫0) manifested an enhanced capillary pressure head. Reduced capillary pressure head was associated with an increase in average conductivity, and enhanced capillary pressure head was associated with a decrease in average conductivity when compared to the average water conductivity during water imbibition. The employed experimental method and mathematical analysis of dynamic flow, subject to interfacial spreading phenomena, are practical for quantifying parameters for use in sharp-front OIL infiltration models, but more research is needed to determine how to incorporate the spreading coefficient in numerical multiphase flow models.

A Time-Series of Surface Oil Distribution Detected by Satellite SAR During the Deepwater Horizon Blowout

NASA Astrophysics Data System (ADS)

MacDonald, I. R.; Garcia-Pineda, O. G.; Solow, A.; Daneshgar, S.; Beet, A.

2013-12-01

Oil discharged as a result of the Deepwater Horizon disaster was detected on the surface of the Gulf of Mexico by synthetic aperture radar satellites from 25 April 2010 until 4 August 2010. SAR images were not restricted by daylight or cloud-cover. Distribution of this material is a tracer for potential environmental impacts and an indicator of impact mitigation due to response efforts and physical forcing factors. We used a texture classifying neural network algorithm for semi-supervised processing of 176 SAR images from the ENVISAT, RADARSAT I, and COSMO-SKYMED satellites. This yielded an estimate the proportion of oil-covered water within the region sampled by each image with a nominal resolution of 10,000 sq m (100m pixels), which was compiled as a 5-km equal area grid covering the northern Gulf of Mexico. Few images covered the entire impact area, so analysis was required to compile a regular time-series of the oil cover. A Gaussian kernel using a bandwidth of 2 d was used to estimate oil cover percent in each grid at noon and midnight throughout the interval. Variance and confidence intervals were calculated for each grid and for the global 12-h totals. Results animated across the impact region show the spread of oil under the influence of physical factors. Oil cover reached an early peak of 17032.26 sq km (sd 460.077) on 18 May, decreasing to 27% of this total on 4 June, following by sharp increase to an overall maximum of 18424.56 sq km (sd 424.726) on 19 June. There was a significant negative correlation between average wind stress and the total area of oil cover throughout the time-series. Correlation between response efforts including aerial and subsurface application of dispersants and burning of gathered oil was negative, positive, or indeterminate at different time segments during the event. Daily totals for oil-covered surface waters of the Gulf of Mexico during 25 April - 9 August 2010 with upper and lower 0.95 confidence limits on estimate. (No oil visible after 4 August.)

Catalytic upgrading of bio-oil produced from hydrothermal liquefaction of Nannochloropsis sp.

PubMed

Shakya, Rajdeep; Adhikari, Sushil; Mahadevan, Ravishankar; Hassan, El Barbary; Dempster, Thomas A

2018-03-01

Upgrading of bio-oil obtained from hydrothermal liquefaction (HTL) of algae is necessary for it to be used as a fuel. In this study, bio-oil obtained from HTL of Nannochloropsis sp. was upgraded using five different catalysts (Ni/C, ZSM-5, Ni/ZSM-5, Ru/C and Pt/C) at 300 °C and 350 °C. The upgraded bio-oil yields were higher at 300 °C; however, higher quality upgraded bio-oils were obtained at 350 °C. Ni/C gave the maximum upgraded bio-oil yield (61 wt%) at 350 °C. However, noble metal catalysts (Ru/C and Pt/C) gave the better upgraded bio-oils in terms of acidity, heating values, and nitrogen values. The higher heating value of the upgraded bio-oils ranged from 40 to 44 MJ/kg, and the nitrogen content decreased from 5.37 to 1.29 wt%. Most of the upgraded bio-oils (35-40 wt%) were in the diesel range. The major components present in the gaseous products were CH 4 , CO, CO 2 and lower alkanes. Copyright © 2017 Elsevier Ltd. All rights reserved.

Activation of Aspen Wood with Carbon Dioxide and Phosphoric Acid for Removal of Total Organic Carbon from Oil Sands Produced Water: Increasing the Yield with Bio-Oil Recycling

PubMed Central

Veksha, Andrei; Bhuiyan, Tazul I.; Hill, Josephine M.

2016-01-01

Several samples of activated carbon were prepared by physical (CO2) and chemical (H3PO4) activation of aspen wood and tested for the adsorption of organic compounds from water generated during the recovery of bitumen using steam assisted gravity drainage. Total organic carbon removal by the carbon samples increased proportionally with total pore volume as determined from N2 adsorption isotherms at −196 °C. The activated carbon produced by CO2 activation had similar removal levels for total organic carbon from the water (up to 70%) to those samples activated with H3PO4, but lower yields, due to losses during pyrolysis and activation. A method to increase the yield when using CO2 activation was proposed and consisted of recycling bio-oil produced from previous runs to the aspen wood feed, followed by either KOH addition (0.48%) or air pretreatment (220 °C for 3 h) before pyrolysis and activation. By recycling the bio-oil, the yield of CO2 activated carbon (after air pretreatment of the mixture) was increased by a factor of 1.3. Due to the higher carbon yield, the corresponding total organic carbon removal, per mass of wood feed, increased by a factor of 1.2 thus improving the overall process efficiency. PMID:28787817

Development of a rapid method for the sequential extraction and subsequent quantification of fatty acids and sugars from avocado mesocarp tissue.

PubMed

Meyer, Marjolaine D; Terry, Leon A

2008-08-27

Methods devised for oil extraction from avocado (Persea americana Mill.) mesocarp (e.g., Soxhlet) are usually lengthy and require operation at high temperature. Moreover, methods for extracting sugars from avocado tissue (e.g., 80% ethanol, v/v) do not allow for lipids to be easily measured from the same sample. This study describes a new simple method that enabled sequential extraction and subsequent quantification of both fatty acids and sugars from the same avocado mesocarp tissue sample. Freeze-dried mesocarp samples of avocado cv. Hass fruit of different ripening stages were extracted by homogenization with hexane and the oil extracts quantified for fatty acid composition by GC. The resulting filter residues were readily usable for sugar extraction with methanol (62.5%, v/v). For comparison, oil was also extracted using the standard Soxhlet technique and the resulting thimble residue extracted for sugars as before. An additional experiment was carried out whereby filter residues were also extracted using ethanol. Average oil yield using the Soxhlet technique was significantly (P < 0.05) higher than that obtained by homogenization with hexane, although the difference remained very slight, and fatty acid profiles of the oil extracts following both methods were very similar. Oil recovery improved with increasing ripeness of the fruit with minor differences observed in the fatty acid composition during postharvest ripening. After lipid removal, methanolic extraction was superior in recovering sucrose and perseitol as compared to 80% ethanol (v/v), whereas mannoheptulose recovery was not affected by solvent used. The method presented has the benefits of shorter extraction time, lower extraction temperature, and reduced amount of solvent and can be used for sequential extraction of fatty acids and sugars from the same sample.

Geology and habitat of oil in Ras Budran field, Gulf of Suez, Egypt

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chowdhary, L.R.; Taha, S.

1987-10-01

The Ras Budran structure at the deepest mappable seismic reflector, the top of the Kareem Formation (middle Miocene), is a broad northeast-southwest-trending anticlinal feature with an anomalous strike nearly at right angles to the main Gulf of Suez trend. Oil is produced from three units of Nubian Formation sandstone from a depth of 11,000-12,000 ft (3352-3657 m). The lower unit of Paleozoic age averages 10% porosity and has up to 200 md in-situ permeability. Wells completed in this unit produce up to 2000 BOPD. In contrast, the sands to the upper two units of the Early Cretaceous have 15-20% porositymore » and up to 700 md permeability. Wells completed in this unit produce 6000-8000 BOPD. The Ras Budran structure was formed primarily during an intra-Rudeis tectonic phase (lower Miocene). Oil migration for accumulation in the structure started in the late Miocene or Pliocene when the Santonian Brown Limestone and the Eocene Thebes Formation, the main source beds in the Gulf of Suez, reached the threshold of oil generation at a burial depth of approximately 10,000 ft (3048 m). At this depth, the organic matter in the source beds had a high transformation ratio (0.10 to 0.15), high yields of C/sub 15+/ soluble organic matter and C/sub 15+/ saturated hydrocarbons, vitrinite reflectance (R/sub 0/) of 0.62%, and a time-temperature index (TTI) value of 15. Oil migration from mature source beds in adjoining lows into low-potential Nubian reservoirs is easily explained by fault planes that acted as conduits for oil migration. 16 figures, 3 tables.« less

30 CFR 203.54 - How does my relief arrangement for an oil and gas lease operate if prices rise sharply?

Code of Federal Regulations, 2011 CFR

2011-07-01

... reference price, you must pay the effective royalty rate on all monthly production. (a) Your current reference price is a weighted average of daily closing prices on the NYMEX for light sweet crude oil and... average of daily closing prices on the NYMEX for light sweet crude oil and natural gas during the...

Effect of dietary fatty acid supplements, varying in fatty acid composition, on milk fat secretion in dairy cattle fed diets supplemented to less than 3% total fatty acids.

PubMed

Stoffel, C M; Crump, P M; Armentano, L E

2015-01-01

Dietary fatty acids can affect both milk fat yield and fatty acid (FA) composition. This relationship is well established when the dietary level of FA exceeds 3% of diet dry matter (DM). We could find no reports directly examining the effects of dietary FA profile on milk fat at levels below 3%. Twenty-four primiparous and 36 multiparous lactating cows were paired by production (1 high with 1 low, within parity) to form 30 experimental units. Pairs were fed 6 diets in five 6×6 balanced Latin squares with 21-d periods, and data were collected during the last 5d of each period. Two control diets were fed: a corn control diet (CC; 29% corn silage, 16% alfalfa silage, 19% corn grain, and 8% distillers grain on a DM basis) containing 1.8% FA; and a low-oil control diet (LOC; 9% corn silage, 35% alfalfa silage, 20% food-grade corn starch, and 8% corn gluten feed on a DM basis) containing 1.2% FA. A portion of the food-grade corn starch in LOC was replaced with 4 different FA supplements to create the 4 treatment diets. Treatments were 1.7% (DM basis) of a 50:50 blend of corn oil and high-linoleic safflower oil (LO), 1.7% high-oleic sunflower oil (OO), 1.7% palm oil (PO), or 1.8% calcium salts of palm fatty acids (PFA). The resultant diets were thus enriched in linoleic (LO), oleic (OO), or palmitic acid (PO and PFA). Dietary treatments did not affect dry matter intake. Addition of any of the fat sources to LOC resulted in increased milk yield, but milk fat yields and milk FA composition were variable for the different treatments. The LO treatment resulted in lower milk fat yield, fat concentration, and C16:0 yield but increased both trans-10 C18:1 and trans-10,cis-12 C18:2 yields compared with the other added FA treatments. Diets PO and PFA resulted in increased milk C16:0 yield and decreased total milk C18 yield compared with OO. Regression analysis revealed a negative coefficient for dietary linoleic acid content over basal (LOC) for both milk short-chain FA yield and C16:0 yield. Dietary linoleic acid content also had a positive coefficient for milk trans-10 C18:1 and trans-10,cis-12 conjugated linoleic acid yield. These results demonstrate that even when total dietary FA are below 3%, free oils rich in linoleic acid can reduce milk fat yield by reducing secretion of milk FA with fewer than 18 carbons. Fatty acid composition of fat supplements is important even at this low level of total dietary fat. Copyright © 2015 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

De novo assembly and transcriptome analysis of five major tissues of Jatropha curcas L. using GS FLX titanium platform of 454 pyrosequencing

PubMed Central

2011-01-01

Background Jatropha curcas L. is an important non-edible oilseed crop with promising future in biodiesel production. However, factors like oil yield, oil composition, toxic compounds in oil cake, pests and diseases limit its commercial potential. Well established genetic engineering methods using cloned genes could be used to address these limitations. Earlier, 10,983 unigenes from Sanger sequencing of ESTs, and 3,484 unique assembled transcripts from 454 pyrosequencing of uncloned cDNAs were reported. In order to expedite the process of gene discovery, we have undertaken 454 pyrosequencing of normalized cDNAs prepared from roots, mature leaves, flowers, developing seeds, and embryos of J. curcas. Results From 383,918 raw reads, we obtained 381,957 quality-filtered and trimmed reads that are suitable for the assembly of transcript sequences. De novo contig assembly of these reads generated 17,457 assembled transcripts (contigs) and 54,002 singletons. Average length of the assembled transcripts was 916 bp. About 30% of the transcripts were longer than 1000 bases, and the size of the longest transcript was 7,173 bases. BLASTX analysis revealed that 2,589 of these transcripts are full-length. The assembled transcripts were validated by RT-PCR analysis of 28 transcripts. The results showed that the transcripts were correctly assembled and represent actively expressed genes. KEGG pathway mapping showed that 2,320 transcripts are related to major biochemical pathways including the oil biosynthesis pathway. Overall, the current study reports 14,327 new assembled transcripts which included 2589 full-length transcripts and 27 transcripts that are directly involved in oil biosynthesis. Conclusion The large number of transcripts reported in the current study together with existing ESTs and transcript sequences will serve as an invaluable genetic resource for crop improvement in jatropha. Sequence information of those genes that are involved in oil biosynthesis could be used for metabolic engineering of jatropha to increase oil content, and to modify oil composition. PMID:21492485

De novo assembly and transcriptome analysis of five major tissues of Jatropha curcas L. using GS FLX titanium platform of 454 pyrosequencing.

PubMed

Natarajan, Purushothaman; Parani, Madasamy

2011-04-15

Jatropha curcas L. is an important non-edible oilseed crop with promising future in biodiesel production. However, factors like oil yield, oil composition, toxic compounds in oil cake, pests and diseases limit its commercial potential. Well established genetic engineering methods using cloned genes could be used to address these limitations. Earlier, 10,983 unigenes from Sanger sequencing of ESTs, and 3,484 unique assembled transcripts from 454 pyrosequencing of uncloned cDNAs were reported. In order to expedite the process of gene discovery, we have undertaken 454 pyrosequencing of normalized cDNAs prepared from roots, mature leaves, flowers, developing seeds, and embryos of J. curcas. From 383,918 raw reads, we obtained 381,957 quality-filtered and trimmed reads that are suitable for the assembly of transcript sequences. De novo contig assembly of these reads generated 17,457 assembled transcripts (contigs) and 54,002 singletons. Average length of the assembled transcripts was 916 bp. About 30% of the transcripts were longer than 1000 bases, and the size of the longest transcript was 7,173 bases. BLASTX analysis revealed that 2,589 of these transcripts are full-length. The assembled transcripts were validated by RT-PCR analysis of 28 transcripts. The results showed that the transcripts were correctly assembled and represent actively expressed genes. KEGG pathway mapping showed that 2,320 transcripts are related to major biochemical pathways including the oil biosynthesis pathway. Overall, the current study reports 14,327 new assembled transcripts which included 2589 full-length transcripts and 27 transcripts that are directly involved in oil biosynthesis. The large number of transcripts reported in the current study together with existing ESTs and transcript sequences will serve as an invaluable genetic resource for crop improvement in jatropha. Sequence information of those genes that are involved in oil biosynthesis could be used for metabolic engineering of jatropha to increase oil content, and to modify oil composition.

Bacterial rhizosphere and endosphere populations associated with grasses and trees to be used for phytoremediation of crude oil contaminated soil.

PubMed

Fatima, Kaneez; Afzal, Muhammad; Imran, Asma; Khan, Qaiser M

2015-03-01

Different grasses and trees were tested for their growth in a crude oil contaminated soil. Three grasses, Lolium perenne, Leptochloa fusca, Brachiaria mutica, and two trees, Lecucaena leucocephala and Acacia ampliceps, were selected to investigate the diversity of hydrocarbon-degrading rhizospheric and endophytic bacteria. We found a higher number of hydrocarbon degrading bacteria associated with grasses than trees and that the endophytic bacteria were taxonomically different from rhizosphere associated bacteria showing their spatial distribution with reference to plant compartment as well as genotype. The rhizospheric soil yielded 22 (59.45 %), root interior yielded 9 (24.32 %) and shoot interior yielded 6 (16.21 %) hydrocarbon-degrading bacteria. These bacteria possessed genes encoding alkane hydroxylase and showed multiple plant growth-promoting activities. Bacillus (48.64 %) and Acinetobacter (18.91 %) were dominant genera found in this study. At 2 % crude oil concentration, all bacterial isolates exhibited 25 %-78 % oil degradation and Acinetobacter sp. strain BRSI56 degraded maximum. Our study suggests that for practical application, support of potential bacteria combined with the grasses is more effective approach than trees to remediate oil contaminated soils.

Extraction of essential oil from baby Java orange (Citrus sinensis) solid waste using water and steam distillation

NASA Astrophysics Data System (ADS)

Dewi, I. A.; Prastyo, A. M.; Wijana, S.

2018-03-01

Baby java orange (Citrus sinensis) is commonly consumed as juice. Processing of baby java orange leaves organic waste which consist of the mesocarp, exocarp, seed, and wall of the orange. Therefore, it is necessary to process baby java orange waste to be valuable products. The purpose of this study was to provide added value to unutilized baby java orange waste, and to find out the pretreatment of time-delay process that maximize the yield of essential oil produced. Essential oil processing can be done by water and steam distillation. The study used randomized block design with one factor namely distillation time-delay process by air drying consisted of 4 levels i.e. the distillation delay for 2, 4, 6, and 8 days. The best treatment was determined based on the yield. The best essential oil from baby java orange waste was obtained from the treatment of distillation delay-process of 8 days. This pretreatment generated yield value of 0.63% with moisture content of 24.21%. By estimating the price of essential oil showed that this effort not only reduced the bulky organic waste but also provided potential economical value.

Processing of centrifuged solids from liquefied coal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Weintraub, M.; Akhtar, S.; Yavorsky, P.M.

The centrifuged residues from coal liquefaction processes present two problems which the Pittsburgh ETC has found to be controllable by thermal processing of the material: the residues contain significant amounts of carbon and oil that should be recovered, and their sticky, malodorous, dough-like nature makes them difficult to handle. This paper reports the results of heat treatments using several types of processing equipment and two different temperature ranges. Pyrolysis in the range of 1,100/sup 0/ to 1,400/sup 0/F yielded 20 to 30 wt % of oil product, depending on the source of the residue and its initial oil content. Lowermore » temperature heating (e.g., 600/sup 0/F) yielded less oil, but did produce a non-sticky friable solid. The percent oil yield was a function of temperature, residence time, and thickness of the layer exposed. A one-eighth inch thick sample became friable in 15 to 30 minutes exposure to 600/sup 0/F, during which time it lost about 13 percent of its weight. The tests included measurements on a steel conveyor belt, which could become the commercial embodiment of this treatment.« less

Catalytic co-pyrolysis of waste vegetable oil and high density polyethylene for hydrocarbon fuel production.

PubMed

Wang, Yunpu; Dai, Leilei; Fan, Liangliang; Cao, Leipeng; Zhou, Yue; Zhao, Yunfeng; Liu, Yuhuan; Ruan, Roger

2017-03-01

In this study, a ZrO 2 -based polycrystalline ceramic foam catalyst was prepared and used in catalytic co-pyrolysis of waste vegetable oil and high density polyethylene (HDPE) for hydrocarbon fuel production. The effects of pyrolysis temperature, catalyst dosage, and HDPE to waste vegetable oil ratio on the product distribution and hydrocarbon fuel composition were examined. Experimental results indicate that the maximum hydrocarbon fuel yield of 63.1wt. % was obtained at 430°C, and the oxygenates were rarely detected in the hydrocarbon fuel. The hydrocarbon fuel yield increased when the catalyst was used. At the catalyst dosage of 15wt.%, the proportion of alkanes in the hydrocarbon fuel reached 97.85wt.%, which greatly simplified the fuel composition and improved the fuel quality. With the augment of HDPE to waste vegetable oil ratio, the hydrocarbon fuel yield monotonously increased. At the HDPE to waste vegetable oil ratio of 1:1, the maximum proportion (97.85wt.%) of alkanes was obtained. Moreover, the properties of hydrocarbon fuel were superior to biodiesel and 0 # diesel due to higher calorific value, better low-temperature low fluidity, and lower density and viscosity. Copyright © 2017 Elsevier Ltd. All rights reserved.

Bio oil synthesis by coupling biological biomass pretreatment and catalytic hydroliquefaction process.

PubMed

Hamieh, S; Beauchet, R; Lemee, L; Toufaily, J; Koubaissy, B; Hamieh, T; Pouilloux, Y; Pinard, L

2014-03-01

The bio-oil synthesis from a mixture of wastes (7wt.% straw, 38wt.% wood, and 45wt.% grass) was carried out by direct liquefaction reaction using Raney Nickel as catalyst and tetralin as solvent. The green wastes were biologically degraded during 3 months. Longer the destructuration time; higher the yield into oil is. Biological pretreatment of green wastes promotes the liquefaction process. Among the components of degraded biomass, Humin, the major fraction (60-80wt.%) that was favored by the biological treatment, yields to a bio oil extremely energetic with a HHV close to biopetroleum (40MJ kg(-1)), contrariwise, Fulvic acids (2-12wt.%), the minor fraction is refractory to liquefaction reaction. Copyright © 2014 Elsevier Ltd. All rights reserved.

Steam distillation extraction kinetics regression models to predict essential oil yield, composition, and bioactivity of chamomile oil

USDA-ARS?s Scientific Manuscript database

Chamomile (Matricaria chamomilla L.) is one of the most widely spread and used medicinal and essential oil crop in the world. Chamomile essential oil is extracted via steam distillation of the inflorescences (flowers). In this study, distillation time (DT) was found to be a crucial determinant of yi...

Study of optimal extraction conditions for achieving high yield and antioxidant activity of tomato seed oil

USDA-ARS?s Scientific Manuscript database

Tomato seeds resulting from tomato processing by-product have not been effectively utilized as value-added products. This study investigated the kinetics of oil extraction from tomato seeds and sought to optimize the oil extraction conditions. The oil was extracted by using hexane as solvent for 0 t...

Foliar essential oils and deer browsing preference of Douglas-fir genotypes

Treesearch

M.A. Radwan

1978-01-01

Yield and composition of essential oils were compared in foliage of Douglas-fir. Five clones with different susceptibilities to deer browsing were used; foliage was collected during the dormant season. There were no qualitative differences among the oils of the different clones, but the oils differed quantitatively in all variables measured. Eight variables appeared...

Implications of Increasing Light Tight Oil Production for U.S. Refining

EIA Publications

2015-01-01

EIA retained Turner, Mason & Company to provide analysis of the implications of increasing domestic light tight oil production for U.S. refining, focusing on regional crude supply/demand balances, refinery crude slates, operations, capital investment, product yields, crude oil exports/imports, petroleum product exports, infrastructure constraints and expansions, and crude oil price relationships.

Management of soybean oil refinery wastes through recycling them for producing biosurfactant using Pseudomonas aeruginosa MR01.

PubMed

Partovi, Maryam; Lotfabad, Tayebe Bagheri; Roostaazad, Reza; Bahmaei, Manochehr; Tayyebi, Shokoufe

2013-06-01

Biosurfactant production through a fermentation process involving the biodegradation of soybean oil refining wastes was studied. Pseudomonas aeruginosa MR01 was able to produce extracellular biosurfactant when it was cultured in three soybean oil refinement wastes; acid oil, deodorizer distillate and soapstock, at different carbon to nitrogen ratios. Subsequent fermentation kinetics in the three types of waste culture were also investigated and compared with kinetic behavior in soybean oil medium. Biodegradation of wastes, biosurfactant production, biomass growth, nitrate consumption and the number of colony forming units were detected in four proposed media, at specified time intervals. Unexpectedly, wastes could stimulate the biodegradation activity of MR01 bacterial cells and thus biosurfactant synthesis beyond that of the refined soybean oil. This is evident from higher yields of biodegradation and production, as revealed in the waste cultures (Ydeg|(Soybean oil) = 53.9 % < Ydeg|(wastes) and YP/S|(wastes) > YP/S|(Soybean oil) = 0.31 g g(-1), respectively). Although production yields were approximately the same in the three waste cultures (YP/S|(wastes) =/~ 0.5 g g(-1)), microbial activity resulted in higher yields of biodegradation (96.5 ± 1.13 %), maximum specific growth rate (μ max = 0.26 ± 0.02 h(-1)), and biosurfactant purity (89.6 %) with a productivity of 14.55 ± 1.10 g l(-1), during the bioconversion of soapstock into biosurfactant. Consequently, applying soybean oil soapstock as a substrate for the production of biosurfactant with commercial value has the potential to provide a combination of economical production with environmental protection through the biosynthesis of an environmentally friendly (green) compound and reduction of waste load entering the environment. Moreover, this work inferred spectrophotometry as an easy method to detect rhamnolipids in the biosurfactant products.

Hydrocarbon Liquid Production from Biomass via Hot-Vapor-Filtered Fast Pyrolysis and Catalytic Hydroprocessing of the Bio-oil

DOE Office of Scientific and Technical Information (OSTI.GOV)

Elliott, Douglas C.; Wang, Huamin; French, Richard

2014-08-14

Hot-vapor filtered bio-oils were produced from two different biomass feedstocks, oak and switchgrass, and the oils were evaluated in hydroprocessing tests for production of liquid hydrocarbon products. Hot-vapor filtering reduced bio-oil yields and increased gas yields. The yields of fuel carbon as bio-oil were reduced by ten percentage points by hot-vapor filtering for both feedstocks. The unfiltered bio-oils were evaluated alongside the filtered bio-oils using a fixed bed catalytic hydrotreating test. These tests showed good processing results using a two-stage catalytic hydroprocessing strategy. Equal-sized catalyst beds, a sulfided Ru on carbon catalyst bed operated at 220°C and a sulfided CoMomore » on alumina catalyst bed operated at 400°C were used with the entire reactor at 100 atm operating pressure. The products from the four tests were similar. The light oil phase product was fully hydrotreated so that nitrogen and sulfur were below the level of detection, while the residual oxygen ranged from 0.3 to 2.0%. The density of the products varied from 0.80 g/ml up to 0.86 g/ml over the period of the test with a correlated change of the hydrogen to carbon atomic ratio from 1.79 down to 1.57, suggesting some loss of catalyst activity through the test. These tests provided the data needed to assess the suite of liquid fuel products from the process and the activity of the catalyst in relationship to the existing catalyst lifetime barrier for the technology.« less

Effect of oil content and kernel processing of corn silage on digestibility and milk production by dairy cows.

PubMed

Weiss, W P; Wyatt, D J

2000-02-01

Corn silages were produced from a high oil corn hybrid and from its conventional hybrid counterpart and were harvested with a standard silage chopper or a chopper equipped with a kernel processing unit. High oil silages had higher concentrations of fatty acids (5.5 vs. 3.4% of dry matter) and crude protein (8.4 vs. 7.5% of dry matter) than the conventional hybrid. Processed silage had larger particle size than unprocessed silage, but more starch was found in small particles for processed silage. Dry matter intake was not influenced by treatment (18.4 kg/d), but yield of fat-corrected milk (23.9 vs. 22.6 kg/d) was increased by feeding high oil silage. Overall, processing corn silage did not affect milk production, but cows fed processed conventional silage tended to produce more milk than did cows fed unprocessed conventional silage. Milk protein percent, but not yield, was reduced with high oil silage. Milk fat percent, but not yield, was higher with processed silage. Overall, processed silage had higher starch digestibility, but the response was much greater for the conventional silage hybrid. The concentration of total digestible nutrients (TDN) tended to be higher for diets with high oil silage (71.6 vs. 69.9%) and tended to be higher for processed silage than unprocessed silage (71.7 vs. 69.8%), but an interaction between variety and processing was observed. Processing conventional corn silage increased TDN to values similar to high oil corn silage but processing high oil corn silage did not influence TDN.

Antioxidant Activity of Essential Oil Extracted by SC-CO₂ from Seeds of Trachyspermum ammi.

PubMed

Singh, Aarti; Ahmad, Anees

2017-07-11

Bcakground: Extracts obtained from natural sources such as plants are of immense importance for humans. Methods: Therefore this study was conducted to obtain essential oil from the seeds of T. ammi by conventional and non-conventional methods. Hydrodistillation (HD), Solvent Extraction (SE), Ultrasonication (US), and Supercritical Carbon-dioxide (SC-CO₂) extraction techniques were used to extract essential oil from the powdered seeds of T. ammi . A quality control method for each extracted oil was developed using HPTLC, FTIR, and GC-MS. The optimization process was carried out using fractional factorial design (FFD) under which three parameters were considered: pressure (150, 175, and 300 bar), temperature (25, 30, and 40 °C), and CO₂ flow rate (5, 10, 15 g/min). Results: The yield of essential oil obtained from the HD, SE, US, and SC-CO₂ methods were 1.20%, 1.82%, 2.30%, and 2.64% v/w , respectively. Antioxidant activity was determined by the DPPH and superoxide scavenging methods and the IC 50 (Inhibition Concentration) values of the T. ammi oil sample were found to be 36.41 and 20.55 µg mL -1 , respectively. Conclusion: The present paper reported that different extraction methods lead to different yields of essential oils and the choice of a suitable method is extremely important to obtain more preferred compounds. The yield was higher in the SC-CO₂ method and it is a sustainable and green extraction technique. Many important constituents were detected in analytical techniques. Antioxidant activities carried out showed that essential oil extracted from T. ammi seeds possess significant antioxidant activity.

Ground-water contamination by crude oil: Section B in U.S. Geological Survey Toxic Substances Hydrology Program: Proceedings of the technical meeting, Charleston, South Carolina, March 8-12, 1999: Volume 3 (Part C)

USGS Publications Warehouse

Delin, G.N.; Herkelrath, W.N.; Morganwalp, David W.; Buxton, Herbert T.

1999-01-01

Ground water contamination by crude oil, and other petroleum-based liquids, is a widespread problem. An average of 83 crude-oil spills occurred per year during 1994-96 in the United States, each spilling an average of about 50,000 barrels of crude oil (U.S. Office of Pipeline Safety, electronic commun., 1997). An understanding of the fate of organic contaminants (such as oil and gasoline) in the subsurface is needed to design innovative and cost-effective remedial solutions at contaminated sites.

Sequential Elution of Essential Oil Constituents during Steam Distillation of Hops (Humulus lupulus L.) and Influence on Oil Yield and Antimicrobial Activity.

PubMed

Jeliazkova, Ekaterina; Zheljazkov, Valtcho D; Kačániova, Miroslava; Astatkie, Tess; Tekwani, Babu L

2018-06-07

The profile and bioactivity of hops (Humulus lupulus L.) essential oil, a complex natural product extracted from cones via steam distillation, depends on genetic and environmental factors, and may also depend on extraction process. We hypothesized that compound mixtures eluted sequentially and captured at different timeframes during the steam distillation process of whole hop cones would have differential chemical and bioactivity profiles. The essential oil was collected sequentially at 8 distillation time (DT) intervals: 0-2, 2-5, 5-10, 10-30, 30-60, 60-120, 120-180, and 180-240 min. The control was a 4-h non-interrupted distillation. Nonlinear regression models described the DT and essential oil compounds relationship. Fractions yielded 0.035 to 0.313% essential oil, while control yielded 1.47%. The oil eluted during the first hour was 83.2%, 9.6% during the second hour, and only 7.2% during the second half of the distillation. Essential oil (EO) fractions had different chemical profile. Monoterpenes were eluted early, while sequiterpenes were eluted late. Myrcene and linalool were the highest in 0-2 min fraction, β-caryophyllene, β-copaene, β-farnesene, and α-humulene were highest in fractions from middle of distillation, whereas α- bergamotene, γ-muurolene, β- and α-selinene, γ- and δ-cadinene, caryophyllene oxide, humulne epoxide II, τ-cadinol, and 6-pentadecen-2-one were highest in 120-180 or 180-240 min fractions. The Gram-negative Escherichia coli was strongly inhibited by essential oil fractions from 2-5 min and 10-30 min, followed by oil fraction from 0-2 min. The strongest inhibition activity against Gram-negative Yersinia enterocolitica, and Gram-positive Clostridium perfringens, Enterococcus faecalis, and Staphylococcus aureus subs. aureus was observed with the control essential oil. This is the first study to describe significant activity of hops essential oils against Trypanosoma brucei, a parasitic protozoan that causes African trypanosomiasis (sleeping sickness in humans and nagana in other animals). Hops essential oil fractions or whole oil may be used as antimicrobial agents or for the development of new drugs.

Fast microwave-assisted catalytic co-pyrolysis of lignin and low-density polyethylene with HZSM-5 and MgO for improved bio-oil yield and quality.

PubMed

Fan, Liangliang; Chen, Paul; Zhang, Yaning; Liu, Shiyu; Liu, Yuhuan; Wang, Yunpu; Dai, Leilei; Ruan, Roger

2017-02-01

Fast microwave-assisted catalytic co-pyrolysis of lignin and low-density polyethylene (LDPE) with HZSM-5 and MgO was investigated. Effects of pyrolysis temperature, lignin to LDPE ratio, MgO to HZSM-5 ratio, and feedstock to catalyst ratio on the products yields and chemical profiles were examined. 500°C was the optimal co-pyrolysis temperature in terms of the maximum bio-oil yield. The proportion of aromatics increased with increasing LDPE content. In addition, with the addition of LDPE (lignin/LDPE=1/2), methoxyl group in the phenols was completely removed. A synergistic effect was found between lignin and LDPE. The proportion of aromatics increased and alkylated phenols decreased with increasing HZSM-5 to MgO ratio. The bio-oil yield increased with the addition of appropriate amount of catalyst and the proportion of alkylated phenols increased with increasing catalyst to feedstock ratio. Copyright © 2016 Elsevier Ltd. All rights reserved.

Effects of Hydrological Parameters on Palm Oil Fresh Fruit Bunch Yield)

NASA Astrophysics Data System (ADS)

Nda, M.; Adnan, M. S.; Suhadak, M. A.; Zakaria, M. S.; Lopa, R. T.

2018-04-01

Climate change effects and variability have been studied by many researchers in diverse geophysical fields. Malaysia produces large volume of palm oil, the effects of climate change on hydrological parameters (rainfall and precipitation) could have adverse effects on palm oil fresh fruit bunch (FFB) production with implications at both local and international market. It is important to understand the effects of climate change on crop yield to adopt new cultivation techniques and guaranteeing food security globally. Based on this background, the paper’s objective is to investigate the effects of rainfall and temperature pattern on crop yield (FFB) within five years period (2013 - 2017) at Batu Pahat District. The Man - Kendall rank technique (trend test) and statistical analyses (correlation and regression) were applied to the dataset used for the study. The results reveal that there are variabilities in rainfall and temperature from one month to the other and the statistical analysis reveals that the hydrological parameters have an insignificant effect on crop yield.

Comparison of Thraustochytrids Aurantiochytrium sp., Schizochytrium sp., Thraustochytrium sp., and Ulkenia sp. for production of biodiesel, long-chain omega-3 oils, and exopolysaccharide.

PubMed

Lee Chang, Kim Jye; Nichols, Carol Mancuso; Blackburn, Susan I; Dunstan, Graeme A; Koutoulis, Anthony; Nichols, Peter D

2014-08-01

Heterotrophic growth of thraustochytrids has potential in coproducing biodiesel for transportation, as well as producing a feedstock for omega-3 long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA), especially docosahexaenoic acid (DHA) for use in nutraceuticals. In this study, we compared eight new endemic Australian thraustochytrid strains from the genera Aurantiochytrium, Schizochytrium, Thraustochytrium, and Ulkenia for the synthesis of exopolysaccharide (EPS), in addition to biodiesel and LC-PUFA. Aurantiochytrium sp. strains readily utilized glucose for biomass production, and increasing glucose from 2 to 4 % w/v of the culture medium resulted in increased biomass yield by an average factor of 1.7. Ulkenia sp. strain TC 010 and Thraustochytrium sp. strain TC 033 did not utilize glucose, while Schizochytrium sp. strain TC 002 utilized less than half the glucose available by day 14, and Thraustochytrium sp. strain TC 004 utilized glucose at 4 % w/v but not 2 % w/v of the culture suggesting a threshold requirement between these values. Across all strains, increasing glucose from 2 to 4 % w/v of the culture medium resulted in increased total fatty acid methyl ester content by an average factor of 1.9. Despite an increasing literature demonstrating the capacity of thraustochytrids for DHA synthesis, the production of EPS from these organisms is not well documented. A broad range of EPS yields was observed. The maximum yield of EPS was observed for Schizochytrium sp. strain TC 002 (299 mg/L). High biomass-producing strains that also have high lipid and high EPS yield may be better candidates for commercial production of biofuels and other coproducts.

The Peanut (Arachis hypogaea L.) Gene AhLPAT2 Increases the Lipid Content of Transgenic Arabidopsis Seeds

PubMed Central

Chen, Silong; Lei, Yong; Xu, Xian; Huang, Jiaquan; Jiang, Huifang; Wang, Jin; Cheng, Zengshu; Zhang, Jianan; Song, Yahui; Liao, Boshou; Li, Yurong

2015-01-01

Lysophosphatidic acid acyltransferase (LPAT), which converts lysophosphatidic acid (LPA) to phosphatidic acid (PA), catalyzes the addition of fatty acyl moieties to the sn-2 position of the LPA glycerol backbone in triacylglycerol (TAG) biosynthesis. We recently reported the cloning and temporal-spatial expression of a peanut (Arachis hypogaea) AhLPAT2gene, showing that an increase in AhLPAT2 transcript levels was closely correlated with an increase in seed oil levels. However, the function of the enzyme encoded by the AhLPAT2 gene remains unclear. Here, we report that AhLPAT2 transcript levels were consistently higher in the seeds of a high-oil cultivar than in those of a low-oil cultivar across different seed developmental stages. Seed-specific overexpression of AhLPAT2 in Arabidopsis results in a higher percentage of oil in the seeds and greater-than-average seed weight in the transgenic plants compared with the wild-type plants, leading to a significant increase in total oil yield per plant. The total fatty acid (FA) content and the proportion of unsaturated FAs also increased. In the developing siliques of AhLPAT2-overexpressing plants, the expression levels of genes encoding crucial enzymes involved in de novo FA synthesis, acetyl-CoA subunit (AtBCCP2) and acyl carrier protein 1 (AtACP1) were elevated. AhLPAT2 overexpression also promoted the expression of several key genes related to TAG assembly, sucrose metabolism, and glycolysis. These results demonstrate that the expression of AhLPAT2 plays an important role in glycerolipid production in peanuts. PMID:26302041

Generation and migration of Bitumen and oil from the oil shale interval of the Eocene Green River formation, Uinta Basin, Utah

USGS Publications Warehouse

Johnson, Ronald C.; Birdwell, Justin E.; Mercier, Tracey J.

2016-01-01

The results from the recent U.S. Geological Survey assessment of in-place oil shale resources of the Eocene Green River Formation, based primarily on the Fischer assay method, are applied herein to define areas where the oil shale interval is depleted of some of its petroleum-generating potential along the deep structural trough of the basin and to make: (1) a general estimates of the amount of this depletion, and (2) estimate the total volume of petroleum generated. Oil yields (gallons of oil per ton of rock, GPT) and in-place oil (barrels of oil per acre, BPA) decrease toward the structural trough of the basin, which represents an offshore lacustrine area that is believed to have originally contained greater petroleum-generating potential than is currently indicated by measured Fischer assay oil yields. Although this interval is considered to be largely immature for oil generation based on vitrinite reflectance measurements, the oil shale interval is a likely source for the gilsonite deposits and much of the tar sands in the basin. Early expulsion of petroleum may have occurred due to the very high organic carbon content and oil-prone nature of the Type I kerogen present in Green River oil shale. In order to examine the possible sources and migration pathways for the tar sands and gilsonite deposits, we have created paleogeographic reconstructions of several oil shale zones in the basin as part of this study.

Wage Inequality and Violent Protests in Oil/Gas Producing Countries

NASA Astrophysics Data System (ADS)

Nuraliyev, Nurlan

This work examines contrasting claims made by academic scholars on the relationship between income inequality and political discontent. Does income inequality directly cause social unrest or is this relationship conditional on the level of democratic development? Using the data from 55 oil/gas producing countries between 2010-2013, the author finds: 1) income disparity between an average income per capita of local population and an average income of foreign labor employed in the oil/gas industry results in higher number of violent protests in more democratic oil/gas producing societies; 2) wage disparity between local and foreign labor in the oil/gas industry is associated with higher number of protests in this industry in more democratic oil/gas producing states.

Laboratory study of the effects of combustion gases on retorting of Green River oil shale with superheated steam

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tyler, A.L.; Bullen, E.A.; Jacobs, H.R.

The leached zone of the Parachute Creek member of the Piceance Basin in the Green River Formation has a unique natural porosity that makes it a likely source for in-situ production of oil from oil shale by injection of superheated steam. The Equity Oil Co. of Salt Lake City, in cooperation with the U. S. Department of Energy, carried out field tests using surface generated steam. Difficulties in delivering steam of sufficiently high temperature to the formation resulted in an experiment which was only marginally successful yielding less than 1 percent of the estimated 300,000 barrels of oil in place.more » In 1981, personnel at Sandia National Laboratory suggested that a downhole steam generator which could produce steam at temperatures in excess of 1000/sup 0/F (538/sup 0/C) at depth could well solve the temperature problem. In order to evaluate the effects of combustion gases which would be injected along with steam, should a downhole steam generator be used, laboratory studies have been completed using steam diluted with CO/sub 2/ and with CO/sub 2/ and N/sub 2/ as the heating medium. Results of experiments in an autoclave reactor and in a laboratory retort are reported. The temperature, residence time, and partial pressure of steam are the parameters which effect oil yield and oil quality. Oil properties are reported for several experimental conditions and include oil yield, boiling point distributions, pour points, gravity, and elemental and hydrocarbon-type analyses. Both the autoclave and laboratory retort experiments indicate that CO/sub 2/ and N/sub 2/ do not take a reactive part in the formation of oils except as they dilute the steam. However, the presence of CO/sub 2/ in the gaseous atmosphere during retorting does promote a low-temperature transformation of dolomite to calcite in the inorganic matrix of the oil shale.« less

Inhibitory effect of essential oils obtained from plants grown in Colombia on yellow fever virus replication in vitro

PubMed Central

Meneses, Rocío; Ocazionez, Raquel E; Martínez, Jairo R; Stashenko, Elena E

2009-01-01

Background An antiviral drug is needed for the treatment of patients suffering from yellow fever. Several compounds present in plants can inactive in vitro a wide spectrum of animal viruses. Aim In the present study the inhibitory effect of essential oils of Lippia alba, Lippia origanoides, Oreganum vulgare and Artemisia vulgaris on yellow fever virus (YFV) replication was investigated. Methods The cytotoxicity (CC50) on Vero cells was evaluated by the MTT reduction method. The minimum concentration of the essential oil that inhibited virus titer by more than 50% (MIC) was determined by virus yield reduction assay. YFV was incubated 24 h at 4°C with essential oil before adsorption on Vero cell, and viral replication was carried out in the absence or presence of essential oil. Vero cells were exposed to essential oil 24 h at 37°C before the adsorption of untreated-virus. Results The CC50 values were less than 100 μg/mL and the MIC values were 3.7 and 11.1 μg/mL. The CC50/MIC ratio was of 22.9, 26.4, 26.5 and 8.8 for L. alba, L origanoides, O. vulgare and A. vulgaris, respectively. The presence of essential oil in the culture medium enhances the antiviral effect: L. origanoides oil at 11.1 μg/mLproduced a 100% reduction of virus yield, and the same result was observed with L. alba, O. vulgare and A. vulgaris oils at100 μg/mL. No reduction of virus yield was observed when Vero cells were treated with essential oil before the adsorption of untreated-virus. Conclusion The essential oils evaluated in the study showed antiviral activities against YFV. The mode of action seems to be direct virus inactivation. PMID:19267922

Kristiina Iisa | NREL

Science.gov Websites

," Topics in Catalysis (2016) "Hydrotreating the Organic Fraction of Biomass Pyrolysis Oil to a Biomass via Hot-Vapor-Filtered Fast Pyrolysis and Catalytic Hydroprocessing of the Bio-oil," Energy & Fuels (2014) "Evaluate Impact of Catalyst Type on Oil Yield and Hydrogen Consumption from

Catalytic pyrolysis of waste furniture sawdust for bio-oil production.

PubMed

Uzun, Başak B; Kanmaz, Gülin

2014-07-01

In this study, the catalytic pyrolysis of waste furniture sawdust in the presence of ZSM-5, H-Y and MCM-41 (10 wt % of the biomass sample) was carried out in order to increase the quality of the liquid product at the various pyrolysis temperatures of 400, 450, 500 and 550(o)C. In the non-catalytic work, the maximum oil yield was obtained as 42% at 500(o)C in a fixed-bed reactor system. In the catalytic work, the maximum oil yield was decreased to 37.48, 30.04 and 29.23% in the presence of ZSM-5, H-Y and MCM-41, respectively. The obtained pyrolysis oils were analyzed by various spectroscopic and chromatographic techniques. It was determined that the use of a catalyst decreased acids and increased valuable organics found in the bio-oil. The removal of oxygen from bio-oil was confirmed with the results of the elemental analysis and gas chromatography-mass spectrometry. © The Author(s) 2014.

Bio-oil from fast pyrolysis of lignin: Effects of process and upgrading parameters.

PubMed

Fan, Liangliang; Zhang, Yaning; Liu, Shiyu; Zhou, Nan; Chen, Paul; Cheng, Yanling; Addy, Min; Lu, Qian; Omar, Muhammad Mubashar; Liu, Yuhuan; Wang, Yunpu; Dai, Leilei; Anderson, Erik; Peng, Peng; Lei, Hanwu; Ruan, Roger

2017-10-01

Effects of process parameters on the yield and chemical profile of bio-oil from fast pyrolysis of lignin and the processes for lignin-derived bio-oil upgrading were reviewed. Various process parameters including pyrolysis temperature, reactor types, lignin characteristics, residence time, and feeding rate were discussed and the optimal parameter conditions for improved bio-oil yield and quality were concluded. In terms of lignin-derived bio-oil upgrading, three routes including pretreatment of lignin, catalytic upgrading, and co-pyrolysis of hydrogen-rich materials have been investigated. Zeolite cracking and hydrodeoxygenation (HDO) treatment are two main methods for catalytic upgrading of lignin-derived bio-oil. Factors affecting zeolite activity and the main zeolite catalytic mechanisms for lignin conversion were analyzed. Noble metal-based catalysts and metal sulfide catalysts are normally used as the HDO catalysts and the conversion mechanisms associated with a series of reactions have been proposed. Copyright © 2017 Elsevier Ltd. All rights reserved.

Optimization of soxhlet extraction and physicochemical analysis of crop oil from seed kernel of Feun Kase (Thevetia peruviana)

NASA Astrophysics Data System (ADS)

Suwari, Kotta, Herry Z.; Buang, Yohanes

2017-12-01

Optimizing the soxhlet extraction of oil from seed kernel of Feun Kase (Thevetia peruviana) for biodiesel production was carried out in this study. The solvent used was petroleum ether and methanol, as well as their combinations. The effect of three factors namely different solvent combinations (polarity), extraction time and extraction temperature were investigated for achieving maximum oil yield. Each experiment was conducted in 250 mL soxhlet apparatus. The physicochemical properties of the oil yield (density, kinematic viscosity, acid value, iodine value, saponification value, and water content) were also analyzed. The optimum conditions were found after 4.5 h with extraction time, extraction temperature at 65 oC and petroleum ether to methanol ratio of 90 : 10 (polarity index 0.6). The oil extract was found to be 51.88 ± 3.18%. These results revealed that the crop oil from seed kernel of Feun Kase (Thevetia peruviana) is a potential feedstock for biodiesel production.

A study of pyrolysis of oil shale of the Leningrad deposit by solid heat carrier

NASA Astrophysics Data System (ADS)

Gerasimov, G. Ya; Khaskhachikh, V. V.; Potapov, O. P.

2017-11-01

The investigation of the oil shale pyrolysis with a solid heat carrier was carried out using the experimental retorting system that simulates the Galoter industrial process. This system allows verifying both fractional composition of the oil shale and solid heat carrier, and their ratio and temperature. The oil shale of the Leningradsky deposit was used in the work, and quartz sand was used as the solid heat carrier. It is shown that the yield of the shale oil under the pyrolysis with solid heat carrier exceeds by more than 20% the results received in the standard Fisher retort. Using ash as the solid heat carrier results in a decrease in the yield of oil and gas with simultaneous increase in the amount of the solid residue. This is due to the chemical interaction of the acid components of the vapor-gas mixture with the oxides of alkaline-earth metals that are part of the ash.

Inhibitors removal from bio-oil aqueous fraction for increased ethanol production.

PubMed

Sukhbaatar, Badamkhand; Li, Qi; Wan, Caixia; Yu, Fei; Hassan, El-Barbary; Steele, Philip

2014-06-01

Utilization of 1,6-anhydro-β-d-glucopyranose (levoglucosan) present (11% w/v) in the water fraction of bio-oil for ethanol production will facilitate improvement in comprehensive utilization of total carbon in biomass. One of the major challenges for conversion of anhydrous sugars from the bio-oil water fraction to bio-ethanol is the presence of inhibitory compounds that slow or impede the microbial fermentation process. Removal of inhibitory compounds was first approached by n-butanol extraction. Optimal ratio of n-butanol and bio-oil water fraction was 1.8:1. Removal of dissolved n-butanol was completed by evaporation. Concentration of sugars in the bio-oil water fraction was performed by membrane filtration and freeze drying. Fermentability of the pyrolytic sugars was tested by fermentation of hydrolyzed sugars with Saccharomyces pastorianus lager yeast. The yield of ethanol produced from pyrolytic sugars in the bio-oil water fraction reached a maximum of 98% of the theoretical yield. Copyright © 2014 Elsevier Ltd. All rights reserved.

Liquid fuel generation from algal biomass via a two-step process: effect of feedstocks.

PubMed

Xu, Yu-Ping; Duan, Pei-Gao; Wang, Feng; Guan, Qing-Qing

2018-01-01

In this study, a two-step processing method (hydrothermal liquefaction followed by catalytic upgrading) was used to produce upgraded bio-oil. A comprehensive screening analysis of algal species, including four microalgae and four macroalgae, was conducted to bridge the gap between previous accounts of microalgae and macroalgae hydrothermal liquefaction and the upgrading process of the resulting crude bio-oils. Hydrothermal liquefaction using eight algal biomasses was performed at 350 °C for 1 h. The microalgae always produced a higher crude bio-oil yield than the macroalgae due to their high lipid content, among which Schizochytrium limacinum provided the maximum crude bio-oil yield of 54.42 wt%. For microalgae, higher amounts of N in the biomass resulted in higher amounts of N in the crude bio-oil; however, contrary results were observed for the macroalgae. The crude bio-oils generated from both the microalgae and macroalgae were characterized as having a high viscosity, total acid number, and heteroatom content, and they were influenced by the biochemical compositions of the feedstocks. Next, all eight-crude bio-oils were treated at 400 °C for 2 h with 10 wt% Ru/C using tetralin as the hydrogen donor. The hydrogen source was provided after tetralin was transformed to naphthalene. All the upgraded bio-oils had higher energy densities and significantly lower N, O, and S contents and viscosities than their corresponding crude bio-oils. However, the H/C molar ratio of the upgraded bio-oils decreased due to the absence of external hydrogen relative to the crude bio-oils. The S content of the upgraded bio-oil produced from upgrading the Schizochytrium limacinum crude bio-oil was even close to the 50 ppm requirement of China IV diesel. Microalgae are better feedstocks than macroalgae for liquid fuel production. Biochemical components have a significant impact on the yield and composition of crude bio-oil. Tetralin does not perform as well as external hydrogen for controlling coke formation. The S content of the upgraded bio-oil can be reduced to 76 ppm for the crude bio-oil produced from Schizochytrium limacinum . Upgraded bio-oils have similar properties to those of naphtha and jet fuel.

Nutritive quality and protein production from grain legumes in a boreal climate.

PubMed

Lizarazo, Clara I; Lampi, Anna-Maija; Liu, Jingwei; Sontag-Strohm, Tuula; Piironen, Vieno; Stoddard, Frederick L

2015-08-15

Boreal cropping systems are heavily focused on the production of small-grain cereals; to improve their resilience to climate change and to achieve food and feed security, diversification is needed. This study investigated the potential of faba bean, narrow-leafed lupin and lentil as protein crops in southern Finland, where faba bean is traditional but the other two are novel. Early cultivars of narrow-leafed lupin and lentil matured adequately. Protein concentration in faba bean was, at 32%, higher than the world average of 29%, while those of narrow-leafed lupin and lentil were close to their world averages. Protein yields decreased in the order faba bean > narrow-leafed lupin > lentil. Lipid content of faba bean and lentil was about 1.2% and that of narrow-leafed lupin about 5.5%, and fatty acid composition was largely oleic and linoleic in all three species. Both lentil and narrow-leafed lupin can be added to the range of feed and food crops produced at high latitudes in Europe. While faba bean produces the greatest protein yield and lysine concentration, the higher sulfur amino acid concentration in lupin, its oil content and its adaptation to acid, sandy soils not suitable for faba bean make it an attractive alternative. © 2014 Society of Chemical Industry.

Characterization of asphaltene molecular structures by cracking under hydrogenation conditions and prediction of the viscosity reduction from visbreaking of heavy oils

NASA Astrophysics Data System (ADS)

Rueda Velasquez, Rosa Imelda

The chemical building blocks that comprise petroleum asphaltenes were determined by cracking samples under conditions that minimized alterations to aromatic and cycloalkyl groups. Hydrogenation conditions that used tetralin as hydrogen-donor solvent, with an iron-based catalyst, allowed asphaltenes from different geological regions to yield 50-60 wt% of distillates (<538°C fraction), with coke yields below 10 wt%. Control experiments with phenanthrene and 5alpha-cholestane confirmed low hydrogenation catalytic activity, and preservation of the cycloalkyl structures. Quantitative recovery of cracking products and characterization of the distillates, by gas chromatography-field ionization--time of flight high resolution mass spectrometry, displayed remarkable similarity in molecular composition for the different asphaltenes. Paraffins and 1-3 ring aromatics were the most abundant building blocks. The diversity of molecules identified, and the high yield of paraffins were consistent with high heterogeneity and complexity of molecules, built up by smaller fragments attached to each other by bridges. The sum of material remaining as vacuum residue and coke was in the range of 35-45 wt%; this total represents the maximum amount of large clusters in asphaltenes that could not be converted to lighter compounds under the evaluated cracking conditions. These analytical data for Cold Lake asphaltenes were transformed into probability density functions that described the molecular weight distributions of the building blocks. These distributions were input for a Monte Carlo approach that allowed stochastic construction of asphaltenes and simulation of their cracking reactions to examine differences in the distributions of products associated to the molecular topology. The construction algorithm evidenced that a significant amount of asphaltenes would consist of 3-5 building blocks. The results did not show significant differences between linear and dendritic molecular architectures, but suggested that dendritic molecules would experience slower reaction rates as they required more breakages to reach a given yield of distillates. Thermal cracking of asphaltenes in heavy oils and bitumens can dramatically reduce viscosity, enabling pipeline transportation with less solvent addition. The viscosities of the products from visbreaking reactions of two different heavy oils were modeled with lumped kinetics based on boiling point pseudo-components, and with the estimation of their individual fluid properties. The model was tuned with experimental viscosity data, and provided estimations of viscosities at different temperatures with absolute average deviations lower than 31%.

Biodiesel production using alkaline ionic liquid and adopted as lubricity additive for low-sulfur diesel fuel.

PubMed

Luo, Hui; Fan, Weiyu; Li, Yang; Nan, Guozhi

2013-07-01

Preparation of biodiesel from vegetable oils, such as rapeseed oil, soybean oil and sunflower oil, catalyzed by an alkaline ionic liquid 1-butyl-3-methylimidazolium imidazolide ([Bmim]Im) was investigated in this work. The results demonstrated that [Bmim]Im exhibited high activity and the yield of biodiesel was up to 95% or more when molar ratio of methanol to vegetable oil was 6:1, ionic liquid dosage was 6 wt.%, reaction temperature was 60°C, and reaction time was 60 min. After [Bmim]Im was used for the sixth time, the yield of biodiesel still remained at about 95%. The effects of the biodiesels on the lubricity of low-sulfur diesel fuel were also investigated using the High Frequency Reciprocating Rig method, and the results showed that sunflower biodiesel and soybean biodiesel had higher lubrication performance than that of rapeseed biodiesel. Copyright © 2012 Elsevier Ltd. All rights reserved.

Fast pyrolysis of oil palm shell (OPS)

NASA Astrophysics Data System (ADS)

Abdullah, Nurhayati; Sulaiman, Fauziah; Aliasak, Zalila

2015-04-01

Biomass is an important renewable source of energy. Residues that are obtained from harvesting and agricultural products can be utilised as fuel for energy generation by conducting any thermal energy conversion technology. The conversion of biomass to bio oil is one of the prospective alternative energy resources. Therefore, in this study fast pyrolysis of oil palm shell was conducted. The main objective of this study was to find the optimum condition for high yield bio-oil production. The experiment was conducted using fixed-bed fluidizing pyrolysis system. The biomass sample was pyrolysed at variation temperature of 450°C - 650°C and at variation residence time of 0.9s - 1.35s. The results obtained were further discussed in this paper. The basic characteristic of the biomass sample was also presented here. The experiment shows that the optimum bio-oil yield was obtained at temperature of 500°C at residence time 1.15s.

Retardation effect of nitrogen compounds and condensed aromatics on shale oil catalytic cracking processing and their characterization.

PubMed

Li, Nan; Chen, Chen; Wang, Bin; Li, Shaojie; Yang, Chaohe; Chen, Xiaobo

Untreated shale oil, shale oil treated with HCl aqueous solution and shale oil treated with HCl and furfural were used to do comparative experiments in fixed bed reactors. Nitrogen compounds and condensed aromatics extracted by HCl and furfural were characterized by electrospray ionization Fourier transform cyclotron resonance mass spectrometry and gas chromatography and mass spectrometry, respectively. Compared with untreated shale oil, the conversion and yield of liquid products increased considerably after removing basic nitrogen compounds by HCl extraction. Furthermore, after removing nitrogen compounds and condensed aromatics by both HCl and furfural, the conversion and yield of liquid products further increased. In addition, N 1 class species are predominant in both basic and non-basic nitrogen compounds, and they are probably indole, carbazole, cycloalkyl-carbazole, pyridine and cycloalkyl-pyridine. As for the condensed aromatics, most of them possess aromatic rings with two to three rings and zero to four carbon atom.

Effect of lithium salts addition on the ionic liquid based extraction of essential oil from Farfarae Flos.

PubMed

Li, Zhen-Yu; Zhang, Sha-Sha; Jie-Xing; Qin, Xue-Mei

2015-01-01

In this study, an ionic liquids (ILs) based extraction approach has been successfully applied to the extraction of essential oil from Farfarae Flos, and the effect of lithium chloride was also investigated. The results indicated that the oil yields can be increased by the ILs, and the extraction time can be reduced significantly (from 4h to 2h), compared with the conventional water distillation. The addition of lithium chloride showed different effect according to the structures of ILs, and the oil yields may be related with the structure of cation, while the chemical compositions of essential oil may be related with the anion. The reduction of extraction time and remarkable higher efficiency (5.41-62.17% improved) by combination of lithium salt and proper ILs supports the suitability of the proposed approach. Copyright © 2014 Elsevier B.V. All rights reserved.

Nannochloropsis algae pyrolysis with ceria-based catalysts for production of high-quality bio-oils.

PubMed

Aysu, Tevfik; Sanna, Aimaro

2015-10-01

Pyrolysis of Nannochloropsis was carried out in a fixed-bed reactor with newly prepared ceria based catalysts. The effects of pyrolysis parameters such as temperature and catalysts on product yields were investigated. The amount of bio-char, bio-oil and gas products, as well as the compositions of the resulting bio-oils was determined. The results showed that both temperature and catalyst had significant effects on conversion of Nannochloropsis into solid, liquid and gas products. The highest bio-oil yield (23.28 wt%) and deoxygenation effect was obtained in the presence of Ni-Ce/Al2O3 as catalyst at 500°C. Ni-Ce/Al2O3 was able to retain 59% of the alga starting energy in the bio-oil, compared to only 41% in absence of catalyst. Lower content of acids and oxygen in the bio-oil, higher aliphatics (62%), combined with HHV show promise for production of high-quality bio-oil from Nannochloropsis via Ni-Ce/Al2O3 catalytic pyrolysis. Copyright © 2015 Elsevier Ltd. All rights reserved.

Pyrolysis of oil palm mesocarp fiber catalyzed with steel slag-derived zeolite for bio-oil production.

PubMed

Kabir, G; Mohd Din, A T; Hameed, B H

2018-02-01

The pyrolysis of oil palm mesocarp fiber (OPMF) was catalyzed with a steel slag-derived zeolite (FAU-SL) in a slow-heating fixed-bed reactor at 450 °C, 550 °C, and 600 °C. The catalytic pyrolysis of OPMF produced a maximum yield of 47 wt% bio-oil at 550 °C, and the crude pyrolysis vapor (CPV) of this process yielded crude pyrolysis oil with broad distribution of bulky oxygenated organic compounds. The bio-oil composition produced at 550 °C contained mainly light and stable acid-rich carbonyls at a relative abundance of 48.02% peak area and phenolic compounds at 12.03% peak area. The FAU-SL high mesoporosity and strong surface acidity caused the conversion of the bulky CPV molecules into mostly light acid-rich carbonyls and aromatics through secondary reactions. The secondary reactions mechanisms facilitated by FAU-SL reduced the distribution of the organic compounds in the bio-oil to mostly acid-rich carbonyls and aromatic in contrast to other common zeolite. Copyright © 2017. Published by Elsevier Ltd.

Alternative to conventional extraction of vetiver oil: Microwave hydrodistillation of essential oil from vetiver roots (Vetiveria zizanioides)

NASA Astrophysics Data System (ADS)

Kusuma, H. S.; Altway, A.; Mahfud, M.

2017-12-01

In this study the extraction of essential oil from vetiver roots (Vetiveria zizanioides) has been carried out by using microwave hydrodistillation. In the extraction of vetiver oil using microwave hydrodistillation method is studied the effect of microwave power, feed to solvent (F/S) ratio and extraction time on the yield of vetiver oil. Besides, in this study can be seen that microwave hydrodistillation method offers important advantages over hydrodistillation, such as shorter extraction time (3 h vs. 24 h for hydrodistillation); better yields (0.49% vs. 0.46% for hydrodistillation); and environmental impact (energy cost is appreciably higher for performing hydrodistillation than that required for extraction using microwave hydrodistillation). Based on the analysis using GC-MS can be seen 19 components on vetiver oil that has been extracted using microwave hydrodistillation. In addition, GC-MS analysis showed that the main components of vetiver oil that has been extracted using microwave hydrodistillation method were β-Gurjunene (30.12%), α-Vetivone (20.12%), 4-(1-cyclohexenyl)-2-trimethylsilymethyl-1-buten-3-yne (13.52%) and δ-Selinene (7.27%).

Comprehension of direct extraction of hydrophilic antioxidants using vegetable oils by polar paradox theory and small angle X-ray scattering analysis.

PubMed

Li, Ying; Fabiano-Tixier, Anne Sylvie; Ruiz, Karine; Rossignol Castera, Anne; Bauduin, Pierre; Diat, Olivier; Chemat, Farid

2015-04-15

Since the polar paradox theory rationalised the fact that polar antioxidants are more effective in nonpolar media, extractions of phenolic compounds in vegetable oils were inspired and achieved in this study for obtaining oils enriched in phenolic compounds. Moreover, the influence of surfactants on the extractability of phenolic compounds was experimentally studied first, followed by the small angle X-ray scattering analysis for the oil structural observation before and after extraction so as to better understand the dissolving mechanism underpinning the extraction. The results showed a significant difference on the extraction yield of phenolic compounds among oils, which was mainly dependent on their composition instead of the unsaturation of fatty acids. Appropriate surfactant additions could significantly improve extraction yield for refined sunflower oils, which 1% w/w addition of glyceryl oleate was determined as the optimal. Besides, 5% w/w addition of lecithin performed the best in oil enrichments compared with mono- and di-glycerides. Copyright © 2014 Elsevier Ltd. All rights reserved.

Box-Behnken design for investigation of microwave-assisted extraction of patchouli oil

NASA Astrophysics Data System (ADS)

Kusuma, Heri Septya; Mahfud, Mahfud

2015-12-01

Microwave-assisted extraction (MAE) technique was employed to extract the essential oil from patchouli (Pogostemon cablin). The optimal conditions for microwave-assisted extraction of patchouli oil were determined by response surface methodology. A Box-Behnken design (BBD) was applied to evaluate the effects of three independent variables (microwave power (A: 400-800 W), plant material to solvent ratio (B: 0.10-0.20 g mL-1) and extraction time (C: 20-60 min)) on the extraction yield of patchouli oil. The correlation analysis of the mathematical-regression model indicated that quadratic polynomial model could be employed to optimize the microwave extraction of patchouli oil. The optimal extraction conditions of patchouli oil was microwave power 634.024 W, plant material to solvent ratio 0.147648 g ml-1 and extraction time 51.6174 min. The maximum patchouli oil yield was 2.80516% under these optimal conditions. Under the extraction condition, the experimental values agreed with the predicted results by analysis of variance. It indicated high fitness of the model used and the success of response surface methodology for optimizing and reflect the expected extraction condition.

Evaluation of methods and marker Systems in Genomic Selection of oil palm (Elaeis guineensis Jacq.).

PubMed

Kwong, Qi Bin; Teh, Chee Keng; Ong, Ai Ling; Chew, Fook Tim; Mayes, Sean; Kulaveerasingam, Harikrishna; Tammi, Martti; Yeoh, Suat Hui; Appleton, David Ross; Harikrishna, Jennifer Ann

2017-12-11

Genomic selection (GS) uses genome-wide markers as an attempt to accelerate genetic gain in breeding programs of both animals and plants. This approach is particularly useful for perennial crops such as oil palm, which have long breeding cycles, and for which the optimal method for GS is still under debate. In this study, we evaluated the effect of different marker systems and modeling methods for implementing GS in an introgressed dura family derived from a Deli dura x Nigerian dura (Deli x Nigerian) with 112 individuals. This family is an important breeding source for developing new mother palms for superior oil yield and bunch characters. The traits of interest selected for this study were fruit-to-bunch (F/B), shell-to-fruit (S/F), kernel-to-fruit (K/F), mesocarp-to-fruit (M/F), oil per palm (O/P) and oil-to-dry mesocarp (O/DM). The marker systems evaluated were simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). RR-BLUP, Bayesian A, B, Cπ, LASSO, Ridge Regression and two machine learning methods (SVM and Random Forest) were used to evaluate GS accuracy of the traits. The kinship coefficient between individuals in this family ranged from 0.35 to 0.62. S/F and O/DM had the highest genomic heritability, whereas F/B and O/P had the lowest. The accuracies using 135 SSRs were low, with accuracies of the traits around 0.20. The average accuracy of machine learning methods was 0.24, as compared to 0.20 achieved by other methods. The trait with the highest mean accuracy was F/B (0.28), while the lowest were both M/F and O/P (0.18). By using whole genomic SNPs, the accuracies for all traits, especially for O/DM (0.43), S/F (0.39) and M/F (0.30) were improved. The average accuracy of machine learning methods was 0.32, compared to 0.31 achieved by other methods. Due to high genomic resolution, the use of whole-genome SNPs improved the efficiency of GS dramatically for oil palm and is recommended for dura breeding programs. Machine learning slightly outperformed other methods, but required parameters optimization for GS implementation.

Interactive effect of supplemental ultraviolet B and elevated ozone on seed yield and oil quality of two cultivars of linseed (Linum usitatissimum L.) carried out in open top chambers.

PubMed

Tripathi, Ruchika; Agrawal, Shashi B

2013-03-30

Current scenarios of global climate change predict a significant increase in ultraviolet B (UV-B) and tropospheric ozone (O₃) in the near future. Both UV-B and O₃ can have detrimental effects on the productivity and yield quality of important agricultural crops. The present study was conducted to investigate the individual and interactive effects of supplemental UV-B (sUV-B) (ambient + 7.2 kJ m⁻² day⁻¹) and O₃ (ambient + 10 ppb) on the yield and oil quality of two cultivars of linseed (Linum usitatissimum L.). The mean monthly ambient O₃ concentration varied from 27.7 to 59.0 ppb during the experimental period. O₃ affected fruit formation, while sUV-B was mainly responsible for ovule abortion. Seed sugar and protein contents showed maximum reduction in O₃-treated plants, while mineral nutrient levels were most affected by sUV-B + O₃ treatment. Rancid oil of low nutritional quality and containing long-chain fatty acids was favoured along with a decrease in oil content. sUV-B and O₃ individually as well as in combination caused deterioration of the yield and quality of oil and seeds of linseed. However, the individual effect of O₃ was more damaging than the effect of sUV-B or sUV-B + O₃, and cultivar T-397 performed better than Padmini. © 2012 Society of Chemical Industry.

Ultraviolet-B and photosynthetically active radiation interactively affect yield and pattern of monoterpenes in leaves of peppermint (Mentha x piperita L.).

PubMed

Behn, Helen; Albert, Andreas; Marx, Friedhelm; Noga, Georg; Ulbrich, Andreas

2010-06-23

Solar radiation is a key environmental signal in regulation of plant secondary metabolism. Since metabolic responses to light and ultraviolet (UV) radiation exposure are known to depend on the ratio of spectral ranges (e.g., UV-B/PAR), we examined effects of different UV-B radiation (280-315 nm) and photosynthetically active radiation (PAR, 400-700 nm) levels and ratios on yield and pattern of monoterpenoid essential oil of peppermint. Experiments were performed in exposure chambers, technically equipped for realistic simulation of natural climate and radiation. The experimental design comprised four irradiation regimes created by the combination of two PAR levels including or excluding UV-B radiation. During flowering, the highest essential oil yield was achieved at high PAR (1150 micromol m(-2) s(-1)) and approximate ambient UV-B radiation (0.6 W m(-2)). Regarding the monoterpene pattern, low PAR (550 micromol m(-2) s(-1)) and the absence of UV-B radiation led to reduced menthol and increased menthone contents and thereby to a substantial decrease in oil quality. Essential oil yield could not be correlated with density or diameter of peltate glandular trichomes, the epidermal structures specialized on biosynthesis, and the accumulation of monoterpenes. The present results lead to the conclusion that production of high quality oils (fulfilling the requirements of the Pharmacopoeia Europaea) requires high levels of natural sunlight. In protected cultivation, the use of UV-B transmitting covering materials is therefore highly recommended.

Environmental and seasonal impacts on the chemical composition of Satureja horvatii Šilić (Lamiaceae) essential oils.

PubMed

Lakušić, Branislava; Ristić, Mihailo; Slavkovska, Violeta; Milenković, Marina; Lakušić, Dmitar

2011-03-01

The chemical composition of the essential oils of Satureja horvatii Šilić from two natural habitats (Mt. Orjen and Mt. Lovćen in Montenegro) and from cultivated plants (Belgrade, Serbia) were characterized. For the latter, plants from the locus classicus, i.e., Orjenske Lokve (Mt. Orjen), were transferred to Belgrade and, after three years of cultivation, the chemical composition of their essential oils at different phenological stages was analyzed. The essential oils were obtained from the aerial parts of the plants by hydrodistillation and analyzed by GC and GC/MS. The yields and chemical compositions of the S. horvatii oils showed significant differences between the plants collected in the natural habitats and those from cultivation, as well as between the plants at different phenological stages. In the populations from the natural habitats, growing in Mediterranean conditions, the most abundant oil constituents were the phenols thymol (63.7% in the samples from Mt. Orjen) or carvacrol (68.1% in the samples from Mt. Lovćen), while the oils from the cultivated plants (Belgrade), growing in continental conditions, were dominated by linalool (up to 65.8 and 55.9% in average). The basic characteristics of the essential oil from plants at the early phenological stage (before flowering) were high percentages of linalool (37.4%), thymol (27.3%), and carvacrol (12.2%). At the stage of flowering, the percentage of linalool (56.6-57.5%) increased, while those of thymol (15.5-15.8%) and carvacrol (1.4-1.5%) significantly decreased. The essential oil of plants in the full stage of fruiting was characterized by the domination of linalool (58.4 and 65.8%) and lower percentages of thymol (7.6 and 1.3%) and carvacrol (0.7 and 0.1%). In conclusion, the oil composition of S. horvatii was found to depend on the pedoclimatic conditions of the habitat and the phenological stage of the plants. Copyright © 2011 Verlag Helvetica Chimica Acta AG, Zürich.

Air quality implications of the Deepwater Horizon oil spill.

PubMed

Middlebrook, Ann M; Murphy, Daniel M; Ahmadov, Ravan; Atlas, Elliot L; Bahreini, Roya; Blake, Donald R; Brioude, Jerome; de Gouw, Joost A; Fehsenfeld, Fred C; Frost, Gregory J; Holloway, John S; Lack, Daniel A; Langridge, Justin M; Lueb, Rich A; McKeen, Stuart A; Meagher, James F; Meinardi, Simone; Neuman, J Andrew; Nowak, John B; Parrish, David D; Peischl, Jeff; Perring, Anne E; Pollack, Ilana B; Roberts, James M; Ryerson, Thomas B; Schwarz, Joshua P; Spackman, J Ryan; Warneke, Carsten; Ravishankara, A R

2012-12-11

During the Deepwater Horizon (DWH) oil spill, a wide range of gas and aerosol species were measured from an aircraft around, downwind, and away from the DWH site. Additional hydrocarbon measurements were made from ships in the vicinity. Aerosol particles of respirable sizes were on occasions a significant air quality issue for populated areas along the Gulf Coast. Yields of organic aerosol particles and emission factors for other atmospheric pollutants were derived for the sources from the spill, recovery, and cleanup efforts. Evaporation and subsequent secondary chemistry produced organic particulate matter with a mass yield of 8 ± 4% of the oil mixture reaching the water surface. Approximately 4% by mass of oil burned on the surface was emitted as soot particles. These yields can be used to estimate the effects on air quality for similar events as well as for this spill at other times without these data. Whereas emission of soot from burning surface oil was large during the episodic burns, the mass flux of secondary organic aerosol to the atmosphere was substantially larger overall. We use a regional air quality model to show that some observed enhancements in organic aerosol concentration along the Gulf Coast were likely due to the DWH spill. In the presence of evaporating hydrocarbons from the oil, NO(x) emissions from the recovery and cleanup operations produced ozone.

Air quality implications of the Deepwater Horizon oil spill

PubMed Central

Middlebrook, Ann M.; Murphy, Daniel M.; Ahmadov, Ravan; Atlas, Elliot L.; Bahreini, Roya; Blake, Donald R.; Brioude, Jerome; de Gouw, Joost A.; Fehsenfeld, Fred C.; Frost, Gregory J.; Holloway, John S.; Lack, Daniel A.; Langridge, Justin M.; Lueb, Rich A.; McKeen, Stuart A.; Meagher, James F.; Meinardi, Simone; Neuman, J. Andrew; Nowak, John B.; Parrish, David D.; Peischl, Jeff; Perring, Anne E.; Pollack, Ilana B.; Roberts, James M.; Ryerson, Thomas B.; Schwarz, Joshua P.; Spackman, J. Ryan; Warneke, Carsten; Ravishankara, A. R.

2012-01-01

During the Deepwater Horizon (DWH) oil spill, a wide range of gas and aerosol species were measured from an aircraft around, downwind, and away from the DWH site. Additional hydrocarbon measurements were made from ships in the vicinity. Aerosol particles of respirable sizes were on occasions a significant air quality issue for populated areas along the Gulf Coast. Yields of organic aerosol particles and emission factors for other atmospheric pollutants were derived for the sources from the spill, recovery, and cleanup efforts. Evaporation and subsequent secondary chemistry produced organic particulate matter with a mass yield of 8 ± 4% of the oil mixture reaching the water surface. Approximately 4% by mass of oil burned on the surface was emitted as soot particles. These yields can be used to estimate the effects on air quality for similar events as well as for this spill at other times without these data. Whereas emission of soot from burning surface oil was large during the episodic burns, the mass flux of secondary organic aerosol to the atmosphere was substantially larger overall. We use a regional air quality model to show that some observed enhancements in organic aerosol concentration along the Gulf Coast were likely due to the DWH spill. In the presence of evaporating hydrocarbons from the oil, NOx emissions from the recovery and cleanup operations produced ozone. PMID:22205764

Production of ethanol and feed by high dry matter hydrolysis and fermentation of palm kernel press cake.

PubMed

Jørgensen, Henning; Sanadi, Anand R; Felby, Claus; Lange, Niels Erik Krebs; Fischer, Morten; Ernst, Steffen

2010-05-01

Palm kernel press cake (PKC) is a residue from palm oil extraction presently only used as a low protein feed supplement. PKC contains 50% fermentable hexose sugars present in the form of glucan and mainly galactomannan. This makes PKC an interesting feedstock for processing into bioethanol or in other biorefinery processes. Using a combination of mannanase, beta-mannosidase, and cellulases, it was possible without any pretreatment to hydrolyze PKC at solid concentrations of 35% dry matter with mannose yields up to 88% of theoretical. Fermentation was tested using Saccharomyces cerevisiae in both a separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) setup. The hydrolysates could readily be fermented without addition of nutrients and with average fermentation yields of 0.43 +/- 0.02 g/g based on consumed mannose and glucose. Employing SSF, final ethanol concentrations of 70 g/kg was achieved in 216 h, corresponding to an ethanol yield of 70% of theoretical or 200 g ethanol/kg PKC. Testing various enzyme mixtures revealed that including cellulases in combination with mannanases significantly improved ethanol yields. Processing PKC to ethanol resulted in a solid residue enriched in protein from 17% to 28%, a 70% increase, thereby potentially making a high-protein containing feed supplement.

Optimization of the degumming process for camellia oil by the use of phospholipase C in pilot-scale system.

PubMed

Jiang, Xiaofei; Chang, Ming; Jin, Qingzhe; Wang, Xingguo

2015-06-01

In present study, phospholipase C (PLC) was applied in camellia oil degumming and the response surface method (RSM) was used to determine the optimum degumming conditions (reaction time, reaction temperature and enzyme dosage) for this enzyme. The optimum conditions for the minimum residual phosphorus content (15.14 mg/kg) and maximum yield of camellia oil (98.2 %) were obtained at reaction temperature 53 ºC, reaction time 2.2 h, PLC dosage 400 mg/kg and pH 5.4. The application of phospholipase A (PLA) - assisted degumming process could further reduce the residual phosphorus content of camellia oil (6.84 mg/kg) to make the oil suitable for physical refining while maintaining the maximal oil yield (98.2 %). These results indicate that PLC degumming process in combination with PLA treatment can be a commercially viable alternative for traditional degumming process. Study on the quality changes of degummed oils showed that the oxidative stability of camellia oil was slightly deceased after the enzymatic treatment, thus more attention should be paid to the oxidative stability in the further application.

Steam distillation extraction of ginger essential oil: Study of the effect of steam flow rate and time process

NASA Astrophysics Data System (ADS)

Fitriady, Muhammad Arifuddin; Sulaswatty, Anny; Agustian, Egi; Salahuddin, Aditama, Deska Prayoga Fauzi

2017-01-01

In Indonesia ginger was usually used as a seasoning for dishes, an ingredient for beverage and a source of herbal medicines. Beside raw usage, ginger can be processed to obtain the essential oil which has many advantages such as proven to be an active antimicrobial and having an antioxidant ability. There are a lot of methods to extract essential oil from ginger, one of which is steam distillation. The aim of the current study was to investigate the effect of variation of time process and steam flow rate in the yield on ginger essential oil steam distillation extraction process. It was found that the best operation condition was 0.35 ml/s as the steam flow rate which yields 2.43% oil. The optimum time process was predicted at 7.5 hours. The composition of the oil was varied depend on the flow rate and every flow rate has its own major component contained in the oil. Curcumene composition in the oil was increased as increased steam flow rate applied, but the composition of camphene was decreased along with the increasing steam flow rate.

Organic petrology of selected oil shale samples from lower Carboniferous Albert Formation, New Brunswick, Canada

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kalkreuth, W.; Macauley, G.

1984-04-01

Incident light microscopy was used to describe maturation and composition of organic material in oil shale samples from the Lower Carboniferous Albert Formation of New Brunswick. The maturation level was determined in normal (white) light by measuring vitrinite reflectance and in fluorescent light by measuring fluorescence spectral of alginite B. Results indicate low to intermediate maturation for all of the samples. Composition was determined by maceral analysis. Alginite B is the major organic component in all samples having significant oil potential. Oil yields obtained from the Fischer Assay process, and oil and gas potentials from Rock-Eval analyses correlate to themore » amounts of alginite B and bituminite determined in the samples. In some of the samples characterized by similar high concentrations of alginite B, decrease in Fischer Assay yields and oil and gas potentials is related to an increase in maturation, as expected by increase in the fluorescence parameter lambda/sub max/ and red/green quotient of alginite B. Incident light microscopy, particularly with fluorescent light, offers a valuable tool for the identification of the organic matter in oil shales and for the evaluation of their oil and gas potentials.« less

Postfledging survival of European starlings exposed as nestlings to an organophosphorus insecticide

USGS Publications Warehouse

Stromborg, K.L.; Grue, C.E.; Nichols, J.D.; Hepp, G.R.; Hines, J.E.; Bourne, H.C.

1988-01-01

To test the hypothesis that exposure to organophosphorus (OP) insecticides reduces postfledging survival of altricial birds, 16-d-old European Starlings (Sturnus vulgaris) were weighed and orally dosed with corn oil containing 6.0 mg of dicrotophos per kilogram of body mass or an equivalent exposure of pure corn oil (controls). Two days later, each survivor was weighed again and patagially tagged for identification after fledging. Resightings of marked fledglings were made during weekly 2-d intensive observations yielding four estimates of postfledging survival. Before fledging, only OP-dosed birds died (18.5%), and OP-dosed survivors lost more mass (5.2%, P = .001) than controls (1.4%) but their masses on day 18 were only slightly lower (2% of control mean, P = .10). Brain cholinesterase activity, a sensitive indicator of OP exposure in birds, was depressed and average of 93% in OP-dosed nestlings that died compared to controls, and an average of 46% in OP-dosed nestlings alive on day 18. Age at fledging, postfledging survival, flocking behavior, and habitat use, however, did not differ between OP-dosed and control birds. The effects of the OP on the nestlings appeared to be rapid, to be reversible in survivors, and did not extend into the postfledging period. In addition, we found no relationship between body mass at fledging and postfledging survival.

Biodegradable products by lipase biocatalysis.

PubMed

Linko, Y Y; Lämsä, M; Wu, X; Uosukainen, E; Seppälä, J; Linko, P

1998-11-18

The interest in the applications of biocatalysis in organic syntheses has rapidly increased. In this context, lipases have recently become one of the most studied groups of enzymes. We have demonstrated that lipases can be used as biocatalyst in the production of useful biodegradable compounds. A number of examples are given. 1-Butyl oleate was produced by direct esterification of butanol and oleic acid to decrease the viscosity of biodiesel in winter use. Enzymic alcoholysis of vegetable oils without additional organic solvent has been little investigated. We have shown that a mixture of 2-ethyl-1-hexyl esters can be obtained in a good yield by enzymic transesterification from rapeseed oil fatty acids for use as a solvent. Trimethylolpropane esters were also similarly synthesized as lubricants. Finally, the discovery that lipases can also catalyze ester syntheses and transesterification reactions in organic solvent systems has opened up the possibility of enzyme catalyzed production of biodegradable polyesters. In direct polyesterification of 1,4-butanediol and sebacic acid, polyesters with a mass average molar mass of the order of 56,000 g mol-1 or higher, and a maximum molar mass of about 130,000 g mol-1 were also obtained by using lipase as biocatalyst. Finally, we have demonstrated that also aromatic polyesters can be synthesized by lipase biocatalysis, a higher than 50,000 g mol-1 mass average molar mass of poly(1,6-hexanediyl isophthalate) as an example.

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

PubMed Central

Wei, Likun; Huang, Xuxiong

2017-01-01

Microalga Nannochloropsis oculata is a promising alternative feedstock for biodiesel. Elevating its oil-yielding capacity is conducive to cost-saving biodiesel production. However, the regulatory processes of multi-factor collaborative stresses (MFCS) on the oil-yielding performance of N. oculata are unclear. The duration effects of MFCS (high irradiation, nitrogen deficiency and elevated iron supplementation) on N. oculata were investigated in an 18-d batch culture. Despite the reduction in cell division, the biomass concentration increased, resulting from the large accumulation of the carbon/energy-reservoir. However, different storage forms were found in different cellular storage compounds, and both the protein content and pigment composition swiftly and drastically changed. The analysis of four biodiesel properties using pertinent empirical equations indicated their progressive effective improvement in lipid classes and fatty acid composition. The variation curve of neutral lipid productivity was monitored with fluorescent Nile red and was closely correlated to the results from conventional methods. In addition, a series of changes in the organelles (e.g., chloroplast, lipid body and vacuole) and cell shape, dependent on the stress duration, were observed by TEM and LSCM. These changes presumably played an important role in the acclimation of N. oculata to MFCS and accordingly improved its oil-yielding performance. PMID:28346505

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martinez-Guerra, Edith; Gude, Veera Gnaneswar, E-mail: gude@cee.msstate.edu

Highlights: • Pulse sonication effect on transesterification of waste vegetable oil was studied. • Effects of ethanol, methanol, and alcohol mixtures on FAMEs yield were evaluated. • Effect of ultrasonic intensity, power density, and its output rates were evaluated. • Alcohol mixtures resulted in higher biodiesel yields due to better solubility. - Abstract: This study reports on the effects of direct pulse sonication and the type of alcohol (methanol and ethanol) on the transesterification reaction of waste vegetable oil without any external heating or mechanical mixing. Biodiesel yields and optimum process conditions for the transesterification reaction involving ethanol, methanol, andmore » ethanol–methanol mixtures were evaluated. The effects of ultrasonic power densities (by varying sample volumes), power output rates (in W), and ultrasonic intensities (by varying the reactor size) were studied for transesterification reaction with ethanol, methanol and ethanol–methanol (50%-50%) mixtures. The optimum process conditions for ethanol or methanol based transesterification reaction of waste vegetable oil were determined as: 9:1 alcohol to oil ratio, 1% wt. catalyst amount, 1–2 min reaction time at a power output rate between 75 and 150 W. It was shown that the transesterification reactions using ethanol–methanol mixtures resulted in biodiesel yields as high as >99% at lower power density and ultrasound intensity when compared to ethanol or methanol based transesterification reactions.« less

Cessation of oil exposure in harlequin ducks after the Exxon Valdez oil spill: Cytochrome P4501A biomarker evidence.

PubMed

Esler, Daniel; Ballachey, Brenda E; Bowen, Lizabeth; Miles, A Keith; Dickson, Rian D; Henderson, John D

2017-05-01

The authors quantified hepatic hydrocarbon-inducible cytochrome P4501A (CYP1A) expression, as ethoxyresorufin-O-deethylase (EROD) activity, in wintering harlequin ducks (Histrionicus histrionicus) captured in Prince William Sound, Alaska (USA), during 2011, 2013, and 2014 (22-25 yr following the 1989 Exxon Valdez oil spill). Average EROD activity was compared between birds from areas oiled by the spill and those from nearby unoiled areas. The present study replicated studies conducted from 1998 to 2009 demonstrating that harlequin ducks using areas oiled in 1989 had elevated EROD activity, indicative of oil exposure, up to 2 decades post spill. In the present study, it was found that average EROD activity during March 2011 was significantly higher in wintering harlequin ducks captured in oiled areas relative to unoiled areas, which the authors interpret to indicate that harlequin ducks continued to be exposed to residual Exxon Valdez oil up to 22 yr after the original spill. However, the 2011 results also indicated reductions in exposure relative to previous years. Average EROD activity in birds from oiled areas was approximately 2 times that in birds from unoiled areas in 2011, compared with observations from 2005 to 2009, in which EROD activity was 3 to 5 times higher in oiled areas. It was also found that average EROD activity during March 2013 and March 2014 was not elevated in wintering harlequin ducks from oiled areas. The authors interpret these findings to indicate that exposure of harlequin ducks to residual Exxon Valdez oil abated within 24 yr after the original spill. The present study finalizes a timeline of exposure, extending over 2 decades, for a bird species thought to be particularly vulnerable to oil contamination in marine environments. Environ Toxicol Chem 2017;36:1294-1300. Published 2016 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America. Published 2016 Wiley Periodicals Inc. on behalf of SETAC. This article is a US government work and, as such, is in the public domain in the United States of America.

Extraction and refining of essential oil from Australian tea tree, Melaleuca alterfornia, and the antimicrobial activity in cosmetic products

NASA Astrophysics Data System (ADS)

Huynh, Q.; Phan, T. D.; Thieu, V. Q. Q.; Tran, S. T.; Do, S. H.

2012-03-01

Tea tree oil (TTO) comes from the leaves of Melaleuca alternifornia that belongs to the myrtle family (Myrtaceae). It is one of the most powerful immune system stimulants and sorts out most viral, bacterial and fungal infections in a snap, while it is great to heal wounds and acnes. In Vietnam, Melaleuca trees can grow on acid land that stretches in a large portion of lands in the Mekong Delta region. So, there are some Melaleuca plantations developed under the Vietnamese government plans of increasing plantation forests now. However, TTO contains various amounts of 1,8-cineole that causes skin irritant. So TTO purification is very necessary. In this study, the purification of TTO that meet International Standard ISO 4730 was carried out via two steps. The first step is steam distillation to obtain crude TTO (terpinen-4-ol 35% v/v) and the average productivity is among 2.37% (v/wet-wt) or 1.23% (v/dry-wt). In the second step, the cleaned TTO is collected by vacuum distillation column and extraction yield of the whole process is about 0.3% (w/w). Besides, high concentration essential oil was applied in the cosmetic products to increase its commercial value.

Temperature influence on the fast pyrolysis of manure samples: char, bio-oil and gases production

NASA Astrophysics Data System (ADS)

Fernandez-Lopez, Maria; Anastasakis, Kostas; De Jong, Wiebren; Valverde, Jose Luis; Sanchez-Silva, Luz

2017-11-01

Fast pyrolysis characterization of three dry manure samples was studied using a pyrolyzer. A heating rate of 600°C/s and a holding time of 10 s were selected to reproduce industrial conditions. The effect of the peak pyrolysis temperature (600, 800 and 1000°C) on the pyrolysis product yield and composition was evaluated. Char and bio-oil were gravimetrically quantified. Scanning electron microscopy (SEM) was used to analyse the char structure. H2, CH4, CO and CO2 were measured by means of gas chromatography (GC). A decrease in the char yield and an increase of the gas yield were observed when temperature increased. From 800°C on, it was observed that the char yield of samples Dig R and SW were constant, which indicated that the primary devolatilization reactions stopped. This fact was also corroborated by GC analysis. The bio-oil yield slightly increased with temperature, showing a maximum of 20.7 and 27.8 wt.% for samples Pre and SW, respectively, whereas sample Dig R showed a maximum yield of 16.5 wt.% at 800°C. CO2 and CO were the main released gases whereas H2 and CH4 production increased with temperature. Finally, an increase of char porosity was observed with temperature.

Analysis Monthly Import of Palm Oil Products Using Box-Jenkins Model

NASA Astrophysics Data System (ADS)

Ahmad, Nurul F. Y.; Khalid, Kamil; Saifullah Rusiman, Mohd; Ghazali Kamardan, M.; Roslan, Rozaini; Che-Him, Norziha

2018-04-01

The palm oil industry has been an important component of the national economy especially the agriculture sector. The aim of this study is to identify the pattern of import of palm oil products, to model the time series using Box-Jenkins model and to forecast the monthly import of palm oil products. The method approach is included in the statistical test for verifying the equivalence model and statistical measurement of three models, namely Autoregressive (AR) model, Moving Average (MA) model and Autoregressive Moving Average (ARMA) model. The model identification of all product import palm oil is different in which the AR(1) was found to be the best model for product import palm oil while MA(3) was found to be the best model for products import palm kernel oil. For the palm kernel, MA(4) was found to be the best model. The results forecast for the next four months for products import palm oil, palm kernel oil and palm kernel showed the most significant decrease compared to the actual data.

Influence of liquid phase on nanoparticle-based giant electrorheological fluid.

PubMed

Gong, Xiuqing; Wu, Jinbo; Huang, Xianxiang; Wen, Weijia; Sheng, Ping

2008-04-23

We show that the chemical structures of silicone oils can have an important role in the giant electrorheological (GER) effect. The interaction between silicone oils and solid nanoparticles is found to significantly influence the ER effect. By increasing the kinematic viscosity of silicone oils, which is a function of siloxane chain length, sol-like, gel-like and clay-like appearances of the constituted ER fluids were observed. Different functional-group-terminated silicone oils were also employed as the dispersing media. Significant differences of yield stress were found. We systematically study the effect of siloxane chain lengths on the permeability of oils traveling through the porous spaces between the particles (using the Washburn method), oils adsorbed on the particles' surface (using FT-IR spectra), as well as their particle size distribution (using dynamic light scattering). Our results indicate the hydrogen bonds are instrumental in linking the silicone oil to GER solid particles, and long chain lengths can enhance the agglomeration of the GER nanoparticles to form large clusters. An optimal oil structure, with hydroxyl-terminated silicone oil and a suitable viscosity, was chosen which can create the highest yield stress of ∼300 kPa under a 5 kV mm(-1) DC electric field.

Molecular characterization and comparison of shale oils generated by different pyrolysis methods using FT-ICR mass spectrometry

USGS Publications Warehouse

Jin, J.M.; Kim, S.; Birdwell, J.E.

2011-01-01

Fourier transform ion cyclotron resonance mass spectrometry (FT ICR-MS) was applied in the analysis of shale oils generated using two different pyrolysis systems under laboratory conditions meant to simulate surface and in situ oil shale retorting. Significant variations were observed in the shale oils, particularly the degree of conjugation of the constituent molecules. Comparison of FT ICR-MS results to standard oil characterization methods (API gravity, SARA fractionation, gas chromatography-flame ionization detection) indicated correspondence between the average Double Bond Equivalence (DBE) and asphaltene content. The results show that, based on the average DBE values and DBE distributions of the shale oils examined, highly conjugated species are enriched in samples produced under low pressure, high temperature conditions and in the presence of water.

Distillation time effect on lavender essential oil yield and composition

USDA-ARS?s Scientific Manuscript database

Lavender (Lavandula angustifolia Mill.) is one of the most widely grown essential oil crops in the world. Commercial extraction of lavender oil is done using steam distillation. The objective of this study was to evaluate the effect of the length of the distillation time (DT) on lavender essential o...

Selected castorbean genotypes evaluated for morphological traits, seed yield, fatty acids, and oil content

USDA-ARS?s Scientific Manuscript database

Castorbean, Ricinus communis, contains oil used in pharmaceutics, cosmetics, soap, shampoo, and as a lubricant. Castorbean is rapidly becoming a biodiesel crop because of its high oil content which ranges between 30 and 60% depending upon extraction proceedures. Two hundred and three castorbean acce...

Gas-liquid countercurrent integration process for continuous biodiesel production using a microporous solid base KF/CaO as catalyst.

PubMed

Hu, Shengyang; Wen, Libai; Wang, Yun; Zheng, Xinsheng; Han, Heyou

2012-11-01

A continuous-flow integration process was developed for biodiesel production using rapeseed oil as feedstock, based on the countercurrent contact reaction between gas and liquid, separation of glycerol on-line and cyclic utilization of methanol. Orthogonal experimental design and response surface methodology were adopted to optimize technological parameters. A second-order polynomial model for the biodiesel yield was established and validated experimentally. The high determination coefficient (R(2)=98.98%) and the low probability value (Pr<0.0001) proved that the model matched the experimental data, and had a high predictive ability. The optimal technological parameters were: 81.5°C reaction temperature, 51.7cm fill height of catalyst KF/CaO and 105.98kPa system pressure. Under these conditions, the average yield of triplicate experiments was 93.7%, indicating the continuous-flow process has good potential in the manufacture of biodiesel. Copyright © 2012 Elsevier Ltd. All rights reserved.

A combined microwave pretreatment/solvent extraction process for the production of oil from palm fruit: optimisation, oil quality and effect of prolonged exposure.

PubMed

Tan, Jason Cx; Chuah, Cheng-Hock; Cheng, Sit-Foon

2017-04-01

Conventional palm oil milling involves multiple stages after fruit collection; in particular, oil clarification introduces water into the pressed oil, which results in a large quantity of wastewater. A combined process of microwave pretreatment and solvent extraction to mill crude palm oil, without introducing water or steam, is described. An excellent yield (up to 30%) of oil was obtained with pretreatment in a 42 L, 1000 W and 2450 MHz microwave oven followed by hexane extraction. The optimum conditions (10 min microwave pretreatment and 12 h solvent extraction) yielded an oil with a low free fatty acid content (<1.0%) and an acceptable anisidine value (<3.0 meq kg -1 ). The oil had a fatty acid composition not resembling those of conventional crude palm oil and crude palm kernel oil. In the pretreatment, the leached oil had 6.3% lauric acid whereas the solvent extracted oil had only 1.5% lauric acid. Among the factors affecting the oil quality, microwave pretreatment affected the oil quality significantly; however, an optimised duration that would ensure high efficiency in solvent extraction also resulted in ruptured fruitlets, although not to the extent of causing excessive oxidation. In fact, microwave pretreatment should exceed 12 min; after only 15 min, the oil had 1-methylcyclopentanol (12.96%), 1-tetradecanol (9.44%), 1-nonadecene (7.22%), nonanal (7.13%) and 1-tridecene (5.09%), which probably arose from the degradation of fibres. Microwave pretreatment represents an alternative milling process for crude palm oil compared with conventional processes in the omission of wet treatment with steam. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Physicochemical properties of bio-oil and biochar produced by fast pyrolysis of stored single-pass corn stover and cobs.

PubMed

Shah, Ajay; Darr, Matthew J; Dalluge, Dustin; Medic, Dorde; Webster, Keith; Brown, Robert C

2012-12-01

Short harvest window of corn (Zea mays) stover necessitates its storage before utilization; however, there is not enough work towards exploring the fast pyrolysis behavior of stored biomass. This study investigated the yields and the physicochemical properties (proximate and ultimate analyses, higher heating values and acidity) of the fast pyrolysis products obtained from single-pass stover and cobs stored either inside a metal building or anaerobically within plastic wraps. Biomass samples were pyrolyzed in a 183 cm long and 2.1cm inner diameter free-fall fast pyrolysis reactor. Yields of bio-oil, biochar and non-condensable gases from different biomass samples were in the ranges of 45-55, 25-37 and 11-17 wt.%, respectively, with the highest bio-oil yield from the ensiled single-pass stover. Bio-oils generated from ensiled single-pass cobs and ensiled single-pass stover were, respectively, the most and the least acidic with the modified acid numbers of 95.0 and 65.2 mg g(-1), respectively. Copyright © 2012 Elsevier Ltd. All rights reserved.

Pyrolysis of cassava rhizome in a counter-rotating twin screw reactor unit.

PubMed

Sirijanusorn, Somsak; Sriprateep, Keartisak; Pattiya, Adisak

2013-07-01

A counter-rotating twin screw reactor unit was investigated for its behaviour in the pyrolysis of cassava rhizome biomass. Several parameters such as pyrolysis temperature in the range of 500-700°C, biomass particle size of <0.6mm, the use of sand as heat transfer medium, nitrogen flow rate of 4-10 L/min and nitrogen pressure of 1-3 bar were thoroughly examined. It was found that the pyrolysis temperature of 550°C could maximise the bio-oil yield (50 wt.%). The other optimum parameters for maximising the bio-oil yield were the biomass particle size of 0.250-0.425 mm, the nitrogen flow rate of 4 L/min and the nitrogen pressure of 2 bar. The use of the heat transfer medium could increase the bio-oil yield to a certain extent. Moreover, the water content of bio-oil produced with the counter-rotating twin screw reactor was relatively low, whereas the solids content was relatively high, compared to some other reactor configurations. Copyright © 2013 Elsevier Ltd. All rights reserved.

The loss of essential oil components induced by the Purge Time in the Pressurized Liquid Extraction (PLE) procedure of Cupressus sempervirens.

PubMed

Dawidowicz, Andrzej L; Czapczyńska, Natalia B; Wianowska, Dorota

2012-05-30

The influence of different Purge Times on the effectiveness of Pressurized Liquid Extraction (PLE) of volatile oil components from cypress plant matrix (Cupressus sempervirens) was investigated, applying solvents of diverse extraction efficiencies. The obtained results show the decrease of the mass yields of essential oil components as a result of increased Purge Time. The loss of extracted components depends on the extrahent type - the greatest mass yield loss occurred in the case of non-polar solvents, whereas the smallest was found in polar extracts. Comparisons of the PLE method with Sea Sand Disruption Method (SSDM), Matrix Solid-Phase Dispersion Method (MSPD) and Steam Distillation (SD) were performed to assess the method's accuracy. Independent of the solvent and Purge Time applied in the PLE process, the total mass yield was lower than the one obtained for simple, short and relatively cheap low-temperature matrix disruption procedures - MSPD and SSDM. Thus, in the case of volatile oils analysis, the application of these methods is advisable. Copyright © 2012 Elsevier B.V. All rights reserved.

Variations in Essential Oil Yield, Composition, and Antioxidant Activity of Different Plant Organs from Blumea balsamifera (L.) DC. at Different Growth Times.

PubMed

Yuan, Yuan; Huang, Mei; Pang, Yu-Xin; Yu, Fu-Lai; Chen, Ce; Liu, Li-Wei; Chen, Zhen-Xia; Zhang, Ying-Bo; Chen, Xiao-Lu; Hu, Xuan

2016-08-05

Blumea balsamifera, also named Ainaxiang, is widely used as an ancient medicinal herb in tropical and subtropical Asia. It is rich in essential oils. In this work the essential oils of B. balsamifera from different plant organs and in different months were extracted, and then analyzed by gas chromatography-mass spectrometry. The results showed that essential oil yield of young leaves was the highest (0.65 mL/100 g), followed by mature leaves (0.57 mL/100 g), and the oil yield was higher in October (0.47 mL/100 g) than other months. A total of 44 compounds were identified, representing 92.64%-96.71% of the oil. Eighteen common chemical components were found among the six plant organs, representing >80% of the oil constituents. l-borneol was the main ingredient in leaves, and its content was the highest in senescent leaves and in December. In the essential oils of young shoots and young stems, the main component was dimethoxydurene. Antioxidant activity was also determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene bleaching (BCB) assays. The results indicated that the β-carotene bleaching activity was far stronger than the DPPH radical-scavenging capacity, and the young leaves and young shoots showed stronger antioxidant activity. Dimethoxydurene, β-caryophyllene, and α-caryophyllene play a positive role in good antioxidant activity, while β-eudesmol, phytol, and tetradecanal play a negative role. The antioxidant activity revealed in this study might help in developing this promising bioresource for use in the medicinal and cosmetic industries.

Antioxidant Activity of Essential Oil Extracted by SC-CO2 from Seeds of Trachyspermum ammi

PubMed Central

Singh, Aarti; Ahmad, Anees

2017-01-01

Bcakground: Extracts obtained from natural sources such as plants are of immense importance for humans. Methods: Therefore this study was conducted to obtain essential oil from the seeds of T. ammi by conventional and non-conventional methods. Hydrodistillation (HD), Solvent Extraction (SE), Ultrasonication (US), and Supercritical Carbon-dioxide (SC-CO2) extraction techniques were used to extract essential oil from the powdered seeds of T. ammi. A quality control method for each extracted oil was developed using HPTLC, FTIR, and GC-MS. The optimization process was carried out using fractional factorial design (FFD) under which three parameters were considered: pressure (150, 175, and 300 bar), temperature (25, 30, and 40 °C), and CO2 flow rate (5, 10, 15 g/min). Results: The yield of essential oil obtained from the HD, SE, US, and SC-CO2 methods were 1.20%, 1.82%, 2.30%, and 2.64% v/w, respectively. Antioxidant activity was determined by the DPPH and superoxide scavenging methods and the IC50 (Inhibition Concentration) values of the T. ammi oil sample were found to be 36.41 and 20.55 µg mL−1, respectively. Conclusion: The present paper reported that different extraction methods lead to different yields of essential oils and the choice of a suitable method is extremely important to obtain more preferred compounds. The yield was higher in the SC-CO2 method and it is a sustainable and green extraction technique. Many important constituents were detected in analytical techniques. Antioxidant activities carried out showed that essential oil extracted from T. ammi seeds possess significant antioxidant activity. PMID:28930268

Transesterification catalyzed by Lipozyme TLIM for biodiesel production from low cost feedstock

NASA Astrophysics Data System (ADS)

Halim, Siti Fatimah Abdul; Hassan, Hamizura; Amri, Nurulhuda; Bashah, Nur Alwani Ali

2015-05-01

The development of new strategies to efficiently synthesize biodiesel is of extreme important. This is because biodiesel has been accepted worldwide as an alternative fuel for diesel engines. Biodiesel as alkyl ester derived from vegetable oil has considerable advantages in terms of environmental protection. The diminishing petroleum reserves are the major driving force for researchers to look for better strategies in producing biodiesel. The main hurdle to commercialization of biodiesel is the cost of the raw material. Biodiesel is usually produced from food-grade vegetable oil that is more expensive than diesel fuel. Therefore, biodiesel produced from food-grade vegetable oil is currently not economically feasible. Use of an inexpensive raw material such as waste cooking palm oil and non edible oil sea mango are an attractive option to lower the cost of biodiesel. This study addresses an alternative method for biodiesel production which is to use an enzymatic approach in producing biodiesel fuel from low cost feedstock waste cooking palm oil and unrefined sea mango oil using immobilized lipase Lipozyme TL IM. tert-butanol was used as the reaction medium, which eliminated both negative effects caused by excessive methanol and glycerol as the byproduct. Two variables which is methanol to oil molar ratio and enzyme loading were examine in a batch system. Transesterification of waste cooking palm oil reach 65% FAME yield (methanol to oil molar ratio 6:1 and 10% Novozyme 435 based on oil weight), while transesterification of sea mango oil can reach 90% FAME yield (methanol to oil molar ratio 6:1 and 10% Lipozyme TLIM based on oil weight).

Evaluation of the rhenium-osmium geochronometer in the Phosphoria petroleum system, Bighorn Basin of Wyoming and Montana, USA

USGS Publications Warehouse

Lillis, Paul G.; Selby, David

2013-01-01

Rhenium-osmium (Re-Os) geochronometry is applied to crude oils derived from the Permian Phosphoria Formation of the Bighorn Basin in Wyoming and Montana to determine whether the radiogenic age reflects the timing of petroleum generation, timing of migration, age of the source rock, or the timing of thermochemical sulfate reduction (TSR). The oils selected for this study are interpreted to be derived from the Meade Peak Phosphatic Shale and Retort Phosphatic Shale Members of the Phosphoria Formation based on oil-oil and oil-source rock correlations utilizing bulk properties, elemental composition, δ13C and δ34S values, and biomarker distributions. The δ34S values of the oils range from -6.2‰ to +5.7‰, with oils heavier than -2‰ interpreted to be indicative of TSR. The Re and Os isotope data of the Phosphoria oils plot in two general trends: (1) the main trend (n = 15 oils) yielding a Triassic age (239 ± 43 Ma) with an initial 187Os/188Os value of 0.85 ± 0.42 and a mean square weighted deviation (MSWD) of 1596, and (2) the Torchlight trend (n = 4 oils) yielding a Miocene age (9.24 ± 0.39 Ma) with an initial 187Os/188Os value of 1.88 ± 0.01 and a MSWD of 0.05. The scatter (high MSWD) in the main-trend regression is due, in part, to TSR in reservoirs along the eastern margin of the basin. Excluding oils that have experienced TSR, the regression is significantly improved, yielding an age of 211 ± 21 Ma with a MSWD of 148. This revised age is consistent with some studies that have proposed Late Triassic as the beginning of Phosphoria oil generation and migration, and does not seem to reflect the source rock age (Permian) or the timing of re-migration (Late Cretaceous to Eocene) associated with the Laramide orogeny. The low precision of the revised regression (±21 Ma) is not unexpected for this oil family given the long duration of generation from a large geographic area of mature Phosphoria source rock, and the possible range in the initial 187Os/188Os values of the Meade Peak and Retort source units. Effects of re-migration may have contributed to the scatter, but thermal cracking and biodegradation likely have had minimal or no effect on the main-trend regression. The four Phosphoria-sourced oils from Torchlight and Lamb fields yield a precise Miocene age Re-Os isochron that may reflect the end of TSR in the reservoir due to cooling below a threshold temperature in the last 10 m.y. from uplift and erosion of overlying rocks. The mechanism for the formation of a Re-Os isotopic relationship in a family of crude oils may involve multiple steps in the petroleum generation process. Bitumen generation from the source rock kerogen may provide a reset of the isotopic chronometer, and incremental expulsion of oil over the duration of the oil window may provide some of the variation seen in 187Re/188Os values from an oil family.

An Efficient Extraction Method for Fragrant Volatiles from Jasminum sambac (L.) Ait.

PubMed

Ye, Qiuping; Jin, Xinyi; Zhu, Xinliang; Lin, Tongxiang; Hao, Zhilong; Yang, Qian

2015-01-01

The sweet smell of aroma of Jasminum sambac (L.) Ait. is releasing while the flowers are blooming. Although components of volatile oil have been extensively studied, there are problematic issues, such as low efficiency of yield, flavour distortion. Here, the subcritical fluid extraction (SFE) was performed to extract fragrant volatiles from activated carbon that had absorbed the aroma of jasmine flowers. This novel method could effectively obtain main aromatic compounds with quality significantly better than solvent extraction (SE). Based on the analysis data with response surface methodology (RSM), we optimized the extraction conditions which consisted of a temperature of 44°C, a solvent-to-material ratio of 3.5:1, and an extraction time of 53 min. Under these conditions, the extraction yield was 4.91%. Furthermore, the key jasmine essence oil components, benzyl acetate and linalool, increase 7 fold and 2 fold respectively which lead to strong typical smell of the jasmine oil. The new method can reduce spicy components which lead to the essential oils smelling sweeter. Thus, the quality of the jasmine essence oil was dramatically improved and yields based on the key component increased dramatically. Our results provide a new effective technique for extracting fragrant volatiles from jasmine flowers.

Optimization of microwave assisted extraction of essential oils from Iranian Rosmarinus officinalis L. using RSM.

PubMed

Akhbari, Maryam; Masoum, Saeed; Aghababaei, Fahimeh; Hamedi, Sepideh

2018-06-01

In this study, the efficiencies of conventional hydro-distillation and novel microwave hydro-distillation methods in extraction of essential oil from Rosemary officinalis leaves have been compared. In order to attain the best yield and also highest quality of the essential oil in the microwave assisted method, the optimal values of operating parameters such as extraction time, microwave irradiation power and water volume to plant mass ratio were investigated using central composite design under response surface methodology. Optimal conditions for obtaining the maximum extraction yield in the microwave assisted method were predicted as follows: extraction time of 85 min, microwave power of 888 W, and water volume to plant mass ratio of 0.5 ml/g. The extraction yield at these predicted conditions was computed as 0.7756%. The qualities of the obtained essential oils under designed experiments were optimized based on total contents of four major compounds (α-pinene, 1,8-cineole, camphor and verbenone) which determined by gas chromatography equipped with mass spectroscopy (GC-MS). The highest essential oil quality (55.87%) was obtained at extraction time of 68 min; microwave irradiation power of 700 W; and water volume to plant mass ratio of zero.

Determination of the volatile and semi-volatile secondary metabolites, and aristolochic acids in Aristolochia ringens Vahl.

PubMed

Stashenko, Elena E; Andrés Ordóñez, Sergio; Marín, Néstor Armando; Martínez, Jairo René

2009-10-01

Volatile and semi-volatile secondary metabolites, as well as aristolochic acids (AA), present in leaves, stems, and flowers of Aristolochia ringens were determined by gas chromatography (GC)-mass spectrometry (MS) and high-performance liquid chromatography (HPLC) methods, respectively. Metabolite isolation was performed using different extraction techniques: microwave-assisted hydrodistillation (MWHD), supercritical fluid extraction, and headspace solid-phase microextraction (HS-SPME). The chemical composition of the extracts and oils was established by GC-MS. The determinations of AAI and AAII were conducted by methanolic extraction of different plant parts followed by HPLC analysis. Essential oil yields from leaves and stems were 0.008 +/- 0.0022% and 0.047 +/- 0.0026%, respectively. Aristolochia ringens flowers did not yield essential oil under MWHD. Sesquiterpene hydrocarbons (66%) were the main compounds in the essential oil isolated from leaves whereas monoterpene hydrocarbons (73%) predominated in the stems essential oil. Yields of extracts isolated by SFE from leaves, stems, and flowers were 4 +/- 1.8%, 1.2 +/- 0.25%, and 4 +/- 1.8%, respectively. In vivo HS-SPME of flowers isolated compounds with known unpleasant smells such as volatile aldehydes and short-chain carboxylic acids. HPLC analysis detected the presence of AAII in the flowers of Aristolochia ringens at a concentration of 610 +/- 47 mg/kg of dried flower.

Improving fatty acid methyl ester production yield in a lipase-catalyzed process using waste frying oils as feedstock.

PubMed

Azócar, Laura; Ciudad, Gustavo; Heipieper, Hermann J; Muñoz, Robinson; Navia, Rodrigo

2010-06-01

The application of waste frying oil (WFO) mixed with rapeseed oil as a feedstock for the effective production of fatty acid methyl esters (FAME) in a lipase-catalyzed process was investigated. The response surface methodology (RSM) was used to optimize the interaction of four variables: the percentage of WFO in the mixed feedstock, the methanol-to-oil ratio, the dosage of Novozym 435 as a catalyst and the temperature. Furthermore, the addition of methanol to the reaction mixture in a second step after 8 h was shown to effectively diminish enzyme inhibition. Using this technique, the model predicted the optimal conditions that would reach 100% FAME, including a methanol-to-oil molar ratio of 3.8:1, 100% (wt) WFO, 15% (wt) Novozym 435 and incubation at 44.5 degrees C for 12 h with agitation at 200 rpm, and verification experiments confirmed the validity of the model. According to the model, the addition of WFO increased FAME production yield, which is largely due to its higher contents of monoacylglycerols, diacylglycerols and free fatty acids (in comparison to rapeseed oil), which are more available substrates for the enzymatic catalysis. Therefore, the replacement of rapeseed oil with WFO in Novozym 435-catalyzed processes could diminish biodiesel production costs since it is a less expensive feedstock that increases the production yield and could be a potential alternative for FAME production on an industrial scale. (c) 2009 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Essential oil yield and composition reflect browsing damage of junipers.

PubMed

Markó, Gábor; Gyuricza, Veronika; Bernáth, Jeno; Altbacker, Vilmos

2008-12-01

The impact of browsing on vegetation depends on the relative density and species composition of browsers. Herbivore density and plant damage can be either site-specific or change seasonally and spatially. For juniper (Juniperus communis) forests of a sand dune region in Hungary, it has been assumed that plant damage investigated at different temporal and spatial scales would reflect selective herbivory. The level of juniper damage was tested for a possible correlation with the concentration of plant secondary metabolites (PSMs) in plants and seasonal changes in browsing pressure. Heavily browsed and nonbrowsed junipers were also assumed to differ in their chemical composition, and the spatial distribution of browsing damage within each forest was analyzed to reveal the main browser. Long-term differences in local browsing pressure were also expected and would be reflected in site-specific age distributions of distant juniper populations. The concentrations of PSMs (essential oils) varied significantly among junipers and seasons. Heavily browsed shrubs contained the lowest oil yield; essential oils were highest in shrubs bearing no damage, indicating that PSMs might contribute to reduce browsing in undamaged shrubs. There was a seasonal fluctuation in the yield of essential oil that was lower in the summer period than in other seasons. Gas chromatography (GC) revealed differences in some essential oil components, suggesting that certain chemicals could have contributed to reduced consumption. The consequential long-term changes were reflected in differences in age distribution between distant juniper forests. These results confirm that both the concentration of PSMs and specific compounds of the essential oil may play a role in selective browsing damage by local herbivores.

Anaerobic digestion of glycerol and co-digestion of glycerol and pig manure.

PubMed

Nuchdang, Sasikarn; Phalakornkule, Chantaraporn

2012-06-30

The potential of glycerol obtained from transesterification of waste cooking oil as a main carbon source for biogas production was investigated. The glycerol was highly contaminated with oils and fats and was pretreated with sulfuric acid. Using a carbon source of glucose as a control, we compared biogas production from the acid-treated glycerol in a synthetic medium and the acid-treated glycerol mixed with pig manure. The anaerobic digestion of acid-treated glycerol with supplement in a synthetic medium was found to be satisfactory at organic loading rates (OLR) between 1.3, 1.6 and 2.6 g chemical oxygen demand (COD) L(-1) d(-1). The maximum methane yield of 0.32 L at Standard temperature and pressure (STP) g(-1) COD removal was achieved at an OLR of 1.6 g COD L(-1) d(-1) and the methane content was 54% on an average. At a higher organic loading rate of 5.4 g COD L(-1) d(-1), the propionic acid to acetic acid ratio was higher than the critical threshold limit for metabolic imbalance. Anaerobic digestion of acid-treated glycerol with pig manure was also investigated at the COD ratio of 80:20 (glycerol:pig manure). The anaerobic digestion of acid-treated glycerol with pig manure was found to be satisfactory at organic loading rates between 1.3, 1.7, 2.9 and 5.0 g COD L(-1) d(-1) in terms of COD reduction (>80%) and methane content of (62% on an average). However, the biogas production rate was found to significantly decrease at the highest load. The maximum methane yield of 0.24 L STP g(-1) COD removal was achieved at an OLR of 1.3 g COD L(-1) d(-1). Copyright © 2012 Elsevier Ltd. All rights reserved.

[Comparison of chemical components of essential oils in needles of Pinus massoniana Lamb and Pinus elliottottii Engelm from Guangxi].

PubMed

Shen, Changmao; Duan, Wengui; Cen, Bo; Tan, Jianhui

2006-11-01

Essential oils were extracted by steam distillation from the needles of Pinus massoniana Lamb and Pinus elliottottii Engelm grown in Guangxi. Various factors such as pine needle dosage and extraction time which may influence the oil yield were investigated. The optimum conditions were found to be as follows: pine needle dosage 700 g, extraction time 5 h. The essential oil yields from the needles of Pinus massoniana Lamb and Pinus elliottottii Engelm were 0.45% and 0.19%, respectively. Moreover, the chemical compositions of the essential oils were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Sixty four components in the essential oil from needle of Pinus massoniana Lamb were separated and twenty of them (98.59%) were identified while seventy three components in the essential oil from needle of Pinus elliottottii Engelm were separated and twenty nine of them (94.23%) were identified. Generally, the compositions of the essential oils from needles of the two varieties were similar but the contents of some compounds differed greatly. Especially, the content of alpha-pinene in the essential oils from Pinus massoniana Lamb needles was 2.6 times as that from Pinus elliottottii Engelm needles, but the content of beta-pinene was less than the latter. Mono- and sesquiterpenes were the main composition of the essential oils from Pinus massoniana Lamb and Pinus elliottottii Engelm needles.

Optimization of Supercritical Carbon Dioxide Extraction of Eucommia ulmoides Seed Oil and Quality Evaluation of the Oil.

PubMed

Zhang, Zhen-Shan; Liu, Yu-Lan; Che, Li-Ming

2018-03-01

Supercritical carbon dioxide extraction (SC-CO 2 ) technology was used to extract oil from Eucommia ulmoides seed. The optimum conditions and significant parameters in SC-CO 2 were obtained using response surface methodology (RSM). The qualities of the extracted oil were evaluated by physicochemical properties, fatty acid composition, vitamin E composition. It was found that the optimum extraction parameters were at pressure of 37 MPa, temperature of 40°C, extraction time of 125 min and CO 2 flow rate of 2.6 SL/min. Pressure, temperature and time were identified as significant parameter effecting on extraction yield. The importance of evaluated parameters decreased in the order of pressure > extraction time > temperature > CO 2 flow rate. GC analysis indicated that E. ulmoides seed oil contained about 61% of linolenic acid and its fatty acid composition was similar with that of flaxseed oil and perilla oil. The content and composition of vitamin E was determined using HPLC. The E. ulmoides seed oil was rich in vitamin E (190.72 mg/100 g), the predominant vitamin E isomers were γ- tocopherol and δ- tocopherol, which accounted for 70.87% and 24.81% of the total vitamin E, respectively. The high yield and good physicochemical properties of extracted oil support the notion that SC-CO 2 technology is an effective technique for extracting oil from E. ulmoides seed.

Chemical Composition of Juniperus communis L. Cone Essential Oil and Its Variability among Wild Populations in Kosovo.

PubMed

Hajdari, Avni; Mustafa, Behxhet; Nebija, Dashnor; Miftari, Elheme; Quave, Cassandra L; Novak, Johannes

2015-11-01

Ripe cones of Juniperus communis L. (Cupressaceae) were collected from five wild populations in Kosovo, with the aim of investigating the chemical composition and natural variation of essential oils between and within wild populations. Ripe cones were collected, air dried, crushed, and the essential oils obtained by hydrodistillation. The essential-oil constituents were identified by GC-FID and GC/MS analyses. The yield of essential oil differed depending on the population origins and ranged from 0.4 to 3.8% (v/w, based on the dry weight). In total, 42 compounds were identified in the essential oils of all populations. The principal components of the cone-essential oils were α-pinene, followed by β-myrcene, sabinene, and D-limonene. Taking into consideration the yield and chemical composition, the essential oil originating from various collection sites in Kosovo fulfilled the minimum requirements for J. communis essential oils of the European Pharmacopoeia. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) were used to determine the influence of the geographical variations on the essential-oil composition. These statistical analyses suggested that the clustering of populations was not related to their geographic location, but rather appeared to be linked to local selective forces acting on the chemotype diversity. Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich.

Flash pyrolysis of forestry residues from the Portuguese Central Inland Region within the framework of the BioREFINA-Ter project.

PubMed

Amutio, Maider; Lopez, Gartzen; Alvarez, Jon; Moreira, Rui; Duarte, Gustavo; Nunes, Joao; Olazar, Martin; Bilbao, Javier

2013-02-01

The feasibility of the valorization by flash pyrolysis of forest shrub wastes, namely bushes (Cytisus multiflorus, Spartium junceum, Acacia dealbata and Pterospartum tridentatum) has been studied in a conical spouted bed reactor operating at 500 °C, with a continuous biomass feed and char removal. High bio-oil yields in the 75-80 wt.% range have been obtained for all of the materials, with char yields between 16 and 23 wt.% and low gas yields (4-5 wt.%). Bio-oils are composed mainly of water (accounting for a concentration in the 34-40 wt.% range in the bio-oil), phenols, ketones, acids and furans, with lower contents of saccharides, aldehydes and alcohols. Although their composition depends on the raw material, the compounds are similar to those obtained with more conventional feedstocks. Copyright © 2012 Elsevier Ltd. All rights reserved.

Bioinoculants: A sustainable approach to maximize the yield of Ethiopian mustard (Brassica carinata L.) under low input of chemical fertilizers.

PubMed

Nosheen, Asia; Bano, Asghari; Ullah, Faizan

2016-02-01

This study aimed to find out the effect of plant growth-promoting rhizobacteria (PGPR; Azospirillum brasilense and Azotobacter vinelandii) either alone or in combination with different doses of nitrogen and phosphate fertilizers on growth, seed yield, and oil quality of Brassica carinata (L.) cv. Peela Raya. PGPR were applied as seed inoculation at 10(6) cells/mL(-1) so that the number of bacterial cells per seed was 2.6 × 10(5) cells/seed. The chemical fertilizers, namely, urea and diammonium phosphate (DAP) were applied in different doses (full dose (urea 160 kg ha(-1) + DAP 180 kg ha(-1)), half dose (urea 80 kg ha(-1) + DAP 90 kg ha(-1)), and quarter dose (urea 40 kg ha(-1) + DAP 45 kg ha(-1)). The chemical fertilizers at full and half dose significantly increased the chlorophyll, carotenoids, and protein content of leaves and the seed yield (in kilogram per hectare) but had no effect on the oil content of seed. The erucic acid (C22:1) content present in the seed was increased. Azospirillum performed better than Azotobacter and its effect was at par with full dose of chemical fertilizers (CFF) for pigments and protein content of leaves when inoculated in the presence of half dose of chemical fertilizers (SPH). The seed yield and seed size were greater. Supplementing Azospirillum with SPH assisted Azospirillum to augment the growth and yield, reduced the erucic acid (C22:1) and glucosinolates contents, and increased the unsaturation in seed oil. It is inferred that A. brasilense could be applied as an efficient bioinoculant for enhancing the growth, seed yield, and oil quality of Ethiopian mustard at low fertilizer costs and sustainable ways. © The Author(s) 2013.

Improvement of bio-oil yield and quality in co-pyrolysis of corncobs and high density polyethylene in a fixed bed reactor at low heating rate

NASA Astrophysics Data System (ADS)

Supramono, D.; Lusiani, S.

2016-11-01

Over the past few decades, interest in developing biomass-derived fuel has been increasing rapidly due to the decrease in fossil fuel reserves. Bio-oil produced by biomass pyrolysis however contains high oxygen compounds resulting in low calorific-value fuel and therefore requiring upgrading. In co-pyrolysis of the feed blend of plastics of High Density Polyethylene (HDPE) and biomass of com cob particles, at some compositions free radicals from plastic decomposition containing more hydrogen radicals are able to bond oxygen radicals originating from biomass to reduce oxygenate compounds in the bio-oil thus increasing bio-oil quality. This phenomenon is usually called synergetic effect. In addition to that, the pattern of heating of the feed blend in the pyrolysis reactor is predicted to affect biooil quality and yield. In a batch reactor, co-pyrolysis of corncobs and HDPE requires low heating rate to reach a peak temperature at temperature rise period followed by heating for some time at peak temperature called holding time at constant temperature period. No research has been carried out to investigate how long holding time is set in co-pyrolysis of plastic and biomass to obtain high yield of bio-oil. Holding time may affect either crosslinking of free radicals in gas phase, which increases char product, or secondary pyrolysis in the gas phase, which increases non-condensable gas in the gas phase of pyrolysis reactor, both of which reduce bio-oil yield. Therefore, holding time of co-pyrolysis affects the mass rate of bio-oil formation as the pyrolysis proceeds and quality of the bio-oil. In the present work, effects of holding time on the yield and quality of bio-oil have been investigated using horizontal fixed bed of the feed blends at heating rate of 5°C, peak temperature of 500°C and N2 flow rate of 700 ml/minute. Holding time was varied from 0 to 70 minutes with 10 minutes interval. To investigate the effects of holding time, the composition of HDPE in the feed blend was varied 0, 50 and 100%, while the synergetic effect was investigated by varying the composition of HDPE in the feed blend 0, 25, 50, 75, and 100%. The results show that synergetic effect for non-oxygenate compound production started to work at 63% HDPE in the feed blend and beyond. It was observed that extension of holding time exceeding 0 minutes allowed increase ofbio-oil production rate followed reduction of the rate. Pyrolysis ofboth the corncob feed and the feed blend containing 50% HDPE equally reached maximum bio-oil production rate at holding time of 50 minutes, while that of HDPE feed at 30 minutes. The result pertaining to holding time indicates that biomass in the feed blend governs crosslinking - secondary pyrolysis in the co-pyrolysis.

The quantitative significance of Syntrophaceae and syntrophic partnerships in methanogenic degradation of crude oil alkanes

PubMed Central

Gray, N D; Sherry, A; Grant, R J; Rowan, A K; Hubert, C R J; Callbeck, C M; Aitken, C M; Jones, D M; Adams, J J; Larter, S R; Head, I M

2011-01-01

Libraries of 16S rRNA genes cloned from methanogenic oil degrading microcosms amended with North Sea crude oil and inoculated with estuarine sediment indicated that bacteria from the genera Smithella (Deltaproteobacteria, Syntrophaceace) and Marinobacter sp. (Gammaproteobacteria) were enriched during degradation. Growth yields and doubling times (36 days for both Smithella and Marinobacter) were determined using qPCR and quantitative data on alkanes, which were the predominant hydrocarbons degraded. The growth yield of the Smithella sp. [0.020 g(cell-C)/g(alkane-C)], assuming it utilized all alkanes removed was consistent with yields of bacteria that degrade hydrocarbons and other organic compounds in methanogenic consortia. Over 450 days of incubation predominance and exponential growth of Smithella was coincident with alkane removal and exponential accumulation of methane. This growth is consistent with Smithella's occurrence in near surface anoxic hydrocarbon degrading systems and their complete oxidation of crude oil alkanes to acetate and/or hydrogen in syntrophic partnership with methanogens in such systems. The calculated growth yield of the Marinobacter sp., assuming it grew on alkanes, was [0.0005 g(cell-C)/g(alkane-C)] suggesting that it played a minor role in alkane degradation. The dominant methanogens were hydrogenotrophs (Methanocalculus spp. from the Methanomicrobiales). Enrichment of hydrogen-oxidizing methanogens relative to acetoclastic methanogens was consistent with syntrophic acetate oxidation measured in methanogenic crude oil degrading enrichment cultures. qPCR of the Methanomicrobiales indicated growth characteristics consistent with measured rates of methane production and growth in partnership with Smithella. PMID:21914097

The quantitative significance of Syntrophaceae and syntrophic partnerships in methanogenic degradation of crude oil alkanes.

PubMed

Gray, N D; Sherry, A; Grant, R J; Rowan, A K; Hubert, C R J; Callbeck, C M; Aitken, C M; Jones, D M; Adams, J J; Larter, S R; Head, I M

2011-11-01

Libraries of 16S rRNA genes cloned from methanogenic oil degrading microcosms amended with North Sea crude oil and inoculated with estuarine sediment indicated that bacteria from the genera Smithella (Deltaproteobacteria, Syntrophaceace) and Marinobacter sp. (Gammaproteobacteria) were enriched during degradation. Growth yields and doubling times (36 days for both Smithella and Marinobacter) were determined using qPCR and quantitative data on alkanes, which were the predominant hydrocarbons degraded. The growth yield of the Smithella sp. [0.020 g(cell-C)/g(alkane-C)], assuming it utilized all alkanes removed was consistent with yields of bacteria that degrade hydrocarbons and other organic compounds in methanogenic consortia. Over 450 days of incubation predominance and exponential growth of Smithella was coincident with alkane removal and exponential accumulation of methane. This growth is consistent with Smithella's occurrence in near surface anoxic hydrocarbon degrading systems and their complete oxidation of crude oil alkanes to acetate and/or hydrogen in syntrophic partnership with methanogens in such systems. The calculated growth yield of the Marinobacter sp., assuming it grew on alkanes, was [0.0005 g(cell-C)/g(alkane-C)] suggesting that it played a minor role in alkane degradation. The dominant methanogens were hydrogenotrophs (Methanocalculus spp. from the Methanomicrobiales). Enrichment of hydrogen-oxidizing methanogens relative to acetoclastic methanogens was consistent with syntrophic acetate oxidation measured in methanogenic crude oil degrading enrichment cultures. qPCR of the Methanomicrobiales indicated growth characteristics consistent with measured rates of methane production and growth in partnership with Smithella. © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.

Effect of drying and co-matrix addition on the yield and quality of supercritical CO₂ extracted pumpkin (Cucurbita moschata Duch.) oil.

PubMed

Durante, Miriana; Lenucci, Marcello S; D'Amico, Leone; Piro, Gabriella; Mita, Giovanni

2014-04-01

In this work a process for obtaining high vitamin E and carotenoid yields by supercritical carbon dioxide (SC-CO₂) extraction from pumpkin (Cucurbita moschata Duch.) is described. The results show that the use of a vacuum oven-dried [residual moisture (∼8%)] and milled (70 mesh sieve) pumpkin flesh matrix increased SC-CO₂ extraction yields of total vitamin E and carotenoids of ∼12.0- and ∼8.5-fold, respectively, with respect to the use of a freeze-dried and milled flesh matrix. The addition of milled (35 mesh) pumpkin seeds as co-matrix (1:1, w/w) allowed a further ∼1.6-fold increase in carotenoid yield, besides to a valuable enrichment of the extracted oil in vitamin E (274 mg/100 g oil) and polyunsaturated fatty acids. These findings encourage further studies in order to scale up the process for possible industrial production of high quality bioactive ingredients from pumpkin useful in functional food or cosmeceutical formulation. Copyright © 2013 Elsevier Ltd. All rights reserved.

Influence of calcium carbonate on extraction yield and quality of extra virgin oil from olive (Olea europaea L. cv. Coratina).

PubMed

Squeo, G; Silletti, R; Summo, C; Paradiso, V M; Pasqualone, A; Caponio, F

2016-10-15

The aim of the research was to evaluate the effect of calcium carbonate (1%, 2%, and 4% of addition) at two different particle sizes (2.7μm and 5.7μm), added at the beginning of the malaxation phase, on both the extraction yield and the quality of oil obtained from Coratina olives at different ripening index. The results showed that calcium carbonate significantly increased the extraction yield of olive oil, more than affecting chemical indices. In particular, for less ripened olives, 1-2% of larger particle size calcium carbonate addiction determined a significant increase of the extraction effectiveness, ranging from 4.0 to 4.9%, while more ripened olives required higher amounts of coadjuvant (2-4% when using the larger particle size and 4% when using the smaller one), with a significant increase of the extraction yield up to 5%. Moreover, an increase of pungent perception was observed in some cases when adding calcium carbonate to more ripened olives. Copyright © 2016 Elsevier Ltd. All rights reserved.

Biodiesel Production from Spent Coffee Grounds

NASA Astrophysics Data System (ADS)

Blinová, Lenka; Bartošová, Alica; Sirotiak, Maroš

2017-06-01

The residue after brewing the spent coffee grounds is an oil-containing waste material having a potential of being used as biodiesel feedstock. Biodiesel production from the waste coffee grounds oil involves collection and transportation of coffee residue, drying, oil extraction, and finally production of biodiesel. Different methods of oil extraction with organic solvents under different conditions show significant differences in the extraction yields. In the manufacturing of biodiesel from coffee oil, the level of reaction completion strongly depends on the quality of the feedstock oil. This paper presents an overview of oil extraction and a method of biodiesel production from spent coffee grounds.

A transgene design for enhancing oil content in Arabidopsis and Camelina seeds

USDA-ARS?s Scientific Manuscript database

Increasing the oil yield is a major objective for oilseed crop improvement. Oil biosynthesis and accumulation are influenced by multiple genes involved in embryo and seed development. The LEAFY COTYLEDON1 (LEC1) is a master regulator of embryo development that also enhances the expression of genes i...

Preparation of fatty acid methyl esters from Osage orange (Maclura pomifera) oil and evaluation as biodiesel

USDA-ARS?s Scientific Manuscript database

Fatty acid methyl esters were prepared in high yield by transesterification of Osage orange (Maclura pomifera) oil. Extracted using supercritical CO2, the crude oil was initially treated with mineral acid and methanol to lower its content of free fatty acids, thus rendering it amenable to homogeneou...

Identification and expression of fructose-1,6-bisphosphate aldolase genes and their relations to oil content in developing seeds of tea oil tree (Camellia oleifera).

PubMed

Zeng, Yanling; Tan, Xiaofeng; Zhang, Lin; Jiang, Nan; Cao, Heping

2014-01-01

Tea oil tree (Camellia oleifera, Co) provides a fine edible oil source in China. Tea oil from the seeds is very beneficial to human health. Fructose-1,6-bisphosphate aldolase (FBA) hydrolyzes fructose-1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, two critical metabolites for oil biosynthesis. The objectives of this study were to identify FBA genes and investigate the relationship between FBA gene expression and oil content in developing seeds of tea oil tree. In this paper, four developmentally up-regulated CoFBA genes were identified in Camellia oleifera seeds based on the transcriptome from two seed developmental stages corresponding to the initiation and peak stages of lipid biosynthesis. The expression of CoFBA genes, along with three key oil biosynthesis genes CoACP, CoFAD2 and CoSAD were analyzed in seeds from eight developmental stages by real-time quantitative PCR. The oil content and fatty acid composition were also analyzed. The results showed that CoFBA and CoSAD mRNA levels were well-correlated with oil content whereas CoFAD2 gene expression levels were correlated with fatty acid composition in Camellia seeds. We propose that CoFBA and CoSAD are two important factors for determining tea oil yield because CoFBA gene controls the flux of key intermediates for oil biosynthesis and CoSAD gene controls the synthesis of oleic acid, which accounts for 80% of fatty acids in tea oil. These findings suggest that tea oil yield could be improved by enhanced expression of CoFBA and CoSAD genes in transgenic plants.

Conversion of polar and non-polar algae oil lipids to fatty acid methyl esters with solid acid catalysts--A model compound study.

PubMed

Asikainen, Martta; Munter, Tony; Linnekoski, Juha

2015-09-01

Bio-based fuels are becoming more and more important due to the depleting fossil resources. The production of biodiesel from algae oil is challenging compared to terrestrial vegetable oils, as algae oil consists of polar fatty acids, such as phospholipids and glycolipids, as well as non-polar triglycerides and free fatty acids common in vegetable oils. It is shown that a single sulphonated solid acid catalyst can perform the esterification and transesterification reactions of both polar and non-polar lipids. In mild reaction conditions (60-70 °C) Nafion NR50 catalyst produces methyl palmitate (FAME) from the palmitic acid derivatives of di-, and tri-glyceride, free fatty acid, and phospholipid with over 80% yields, with the glycolipid derivative giving nearly 40% yields of FAME. These results demonstrate how the polar and non-polar lipid derivatives of algal oil can be utilised as feedstocks for biodiesel production with a single catalyst in one reaction step. Copyright © 2015 Elsevier Ltd. All rights reserved.

Preliminary investigation on the production of fuels and bio-char from Chlamydomonas reinhardtii biomass residue after bio-hydrogen production.

PubMed

Torri, Cristian; Samorì, Chiara; Adamiano, Alessio; Fabbri, Daniele; Faraloni, Cecilia; Torzillo, Giuseppe

2011-09-01

The aim of this work was to investigate the potential conversion of Chlamydomonas reinhardtii biomass harvested after hydrogen production. The spent algal biomass was converted into nitrogen-rich bio-char, biodiesel and pyrolysis oil (bio-oil). The yield of lipids (algal oil), obtained by solvent extraction, was 15 ± 2% w/w(dry-biomass). This oil was converted into biodiesel with a 8.7 ± 1% w/w(dry-biomass) yield. The extraction residue was pyrolysed in a fixed bed reactor at 350 °C obtaining bio-char as the principal fraction (44 ± 1% w/w(dry-biomass)) and 28 ± 2% w/w(dry-biomass) of bio-oil. Pyrolysis fractions were characterized by elemental analysis, while the chemical composition of bio-oil was fully characterized by GC-MS, using various derivatization techniques. Energy outputs resulting from this approach were distributed in hydrogen (40%), biodiesel (12%) and pyrolysis fractions (48%), whereas bio-char was the largest fraction in terms of mass. Copyright © 2011 Elsevier Ltd. All rights reserved.

Safety evaluation of wild apricot oil.

PubMed

Gandhi, V M; Mulky, M J; Mukerji, B; Iyer, V J; Cherian, K M

1997-06-01

Wild apricot, a variety of Prunus armeniaca, grows in the hilly regions of India. The seeds yield 27% of kernels. The potential availability of the kernels is 40,000 tons/year and these yield 47% of oil. The oil has 94% unsaturated fatty acids, rich in oleic and linoleic acids. Systemic effects and nutritional quality of wild apricot oil (WAO) were assessed in a 13-wk feeding study in weanling albino rats using a diet containing 10% WAO as the sole source of dietary fat. A similar diet containing groundnut oil (GNO) was used as the control. WAO did not manifest any toxic potential. The food consumption, growth rate and food efficiency ratio of rats fed WAO were similar to those fed GNO. The digestibility of this oil was found to be comparable to that of GNO. There were no macroscopic or microscopic lesions in any of the organs that could be ascribed to WAO incorporation in the diet. The results of this study indicate that WAO could be used for edible purposes without any overt toxic signs or symptoms. However a long-term study may be needed to confirm its innocuousness further.

Effect of torrefaction on structure and fast pyrolysis behavior of corncobs.

PubMed

Zheng, Anqing; Zhao, Zengli; Chang, Sheng; Huang, Zhen; Wang, Xiaobo; He, Fang; Li, Haibin

2013-01-01

Pretreatment of corncobs using torrefaction was conducted in an auger reactor at 250-300 °C and residence times of 10-60 min. The torrefied corncobs were fast pyrolyzed in a bubbling fluidized bed reactor at 470 °C to obtain high-quality bio-oil. The heating value and pH of the bio-oil improved when the torrefaction as pretreatment was applied; however, increasing bio-oil yield penalties were observed with increasing torrefaction severity. Fourier transform infrared Spectroscopy (FTIR) and quantitative solid (13)C nuclear magnetic resonance spectrometry (NMR) analysis of torrefied corncobs showed that the devolatilization, crosslinking and charring of corncobs during torrefaction could be responsible for the bio-oil yield penalties. Gas chromatography-mass spectrometry (GC-MS) analysis showed that the acetic acid and furfural contents of the bio-oil decreased with torrefaction temperature or residence time. The results showed that torrefaction is an effective method of pretreatment for improving bio-oil quality if the crosslinking and charring of biomass can be restricted. Copyright © 2012 Elsevier Ltd. All rights reserved.

Optimisation of Microwave-Assisted Extraction of Pomegranate (Punica granatum L.) Seed Oil and Evaluation 
of Its Physicochemical and Bioactive Properties.

PubMed

Çavdar, Hasene Keskin; Yanık, Derya Koçak; Gök, Uğur; Göğüş, Fahrettin

2017-03-01

Pomegranate seed oil was extracted in a closed-vessel high-pressure microwave system. The characteristics of the obtained oil, such as fatty acid composition, free fatty acidity, total phenolic content, antioxidant activity and colour, were compared to those of the oil obtained by cold solvent extraction. Response surface methodology was applied to optimise extraction conditions: power (176-300 W), time (5-20 min), particle size ( d =0.125-0.800 mm) and solvent to sample ratio (2:1, 6:1 and 10:1, by mass). The predicted highest extraction yield (35.19%) was obtained using microwave power of 220 W, particle size in the range of d =0.125-0.450 mm and solvent-to-sample ratio of 10:1 (by mass) in 5 min extraction time. Microwave-assisted solvent extraction (MASE) resulted in higher extraction yield than that of Soxhlet (34.70% in 8 h) or cold (17.50% in 8 h) extraction. The dominant fatty acid of pomegranate seed oil was punicic acid (86%) irrespective of the extraction method. Oil obtained by MASE had better physicochemical properties, total phenolic content and antioxidant activity than the oil obtained by cold solvent extraction.

The oil palm Shell gene controls oil yield and encodes a homologue of SEEDSTICK

PubMed Central

Singh, Rajinder; Leslie Low, Eng-Ti; Ooi, Leslie Cheng-Li; Ong-Abdullah, Meilina; Chin, Ting Ngoot; Nagappan, Jayanthi; Nookiah, Rajanaidu; Amiruddin, Mohd Din; Rosli, Rozana; Abdul Manaf, Mohamad Arif; Chan, Kuang-Lim; Halim, Mohd Amin; Azizi, Norazah; Lakey, Nathan; Smith, Steven W; Budiman, Muhammad A; Hogan, Michael; Bacher, Blaire; Van Brunt, Andrew; Wang, Chunyan; Ordway, Jared M; Sambanthamurthi, Ravigadevi; Martienssen, Robert A

2014-01-01

A key event in the domestication and breeding of the oil palm, Elaeis guineensis, was loss of the thick coconut-like shell surrounding the kernel. Modern E. guineensis has three fruit forms, dura (thick-shelled), pisifera (shell-less) and tenera (thin-shelled), a hybrid between dura and pisifera1–4. The pisifera palm is usually female-sterile but the tenera yields far more oil than dura, and is the basis for commercial palm oil production in all of Southeast Asia5. Here, we describe the mapping and identification of the Shell gene responsible for the different fruit forms. Using homozygosity mapping by sequencing we found two independent mutations in the DNA binding domain of a homologue of the MADS-box gene SEEDSTICK (STK) which controls ovule identity and seed development in Arabidopsis. The Shell gene is responsible for the tenera phenotype in both cultivated and wild palms from sub-Saharan Africa, and our findings provide a genetic explanation for the single gene heterosis attributed to Shell, via heterodimerization. This gene mutation explains the single most important economic trait in oil palm, and has implications for the competing interests of global edible oil production, biofuels and rainforest conservation6. PMID:23883930

The effects of temperature and catalysts on the pyrolysis of industrial wastes (herb residue).

PubMed

Wang, Pan; Zhan, Sihui; Yu, Hongbing; Xue, Xufang; Hong, Nan

2010-05-01

Pyrolysis of herb residue was investigated in a fixed-bed to determine the effects of pyrolysis temperature and catalysts (ZSM-5, Al-SBA-15 and alumina) on the products yields and the qualities of bio-oils. The results indicated that the maximum bio-oil yield of 34.26% was obtained at 450 degrees Celsius with 10 wt.% alumina catalyst loaded. The pyrolytic oils were examined by ultimate analysis and calorific values determination, and the results indicated that the presence of all catalysts decreased the oxygen content of bio-oils and increased the calorific values. The order of the catalytic effect for upgrading the pyrolytic oil was Al(2)O(3)>Al-SBA-15>ZSM-5. The bio-oil with the lowest oxygen content (26.71%) and the highest calorific value (25.94 MJ kg(-1)) was obtained with 20 wt.% alumina catalyst loaded. Furthermore, the gas chromatography/mass spectrometry (GC/MS) was used in order to investigate the components of obtained pyrolytic oils. It was found that the alumina catalyst could clearly enhance the formation of aliphatics and aromatics. Crown Copyright 2009. Published by Elsevier Ltd. All rights reserved.

Microwave-Assisted Simplified Simultaneous Distillation Coupled with Ionic Liquid Pretreatment for the Analysis of Essential Oil in Schisandra sphenanthera.

PubMed

Yu, Guo-Wei; Nie, Jing; Song, Zhi-Yu; Li, Zu-Guang; Lee, Maw-Rong; Wang, Shen-Peng

2017-11-01

Simultaneous distillation extraction (SDE) is quite useful for the separation of volatile compounds from an analyte when their contents are quite low. In this study, a simplified SDE approach is applied for the extraction of essential oil from Schisandra sphenanthera, with microwave as heating source, [Bmim][Cl] as the medium for pretreatment, and gas chromatography-mass spectrometry as the analytical approach. Consequently, the improvement resulted from [Bmim][Cl] pretreatment is demonstrated by taking comparison with blank experiments. Totally 61 compounds have been detected in the essential oil obtained by using [Bmim][Cl] pretreatment, while without [Bmim][Cl] pretreatment, only 53 compounds can be detected. Moreover, [Bmim][Cl] pretreatment can also resulted in a higher yield of essential oil. The experimental results demonstrate that the simplified SDE coupled with ionic liquid pretreatment is a feasible approach for the extraction of essential oil from S. sphenanthera with high efficiency as 0.85% of essential oil yield has been obtained, and can be potentially extended to the extraction of essential oil or other target volatile compounds with low content. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

Ionic liquids as a reaction medium for lipase-catalyzed methanolysis of sunflower oil.

PubMed

Sunitha, S; Kanjilal, S; Reddy, P S; Prasad, R B N

2007-12-01

Ionic liquids, 1-butyl-3-methyl imidazolium hexafluorophosphate ([BMIm][PF(6)]) and 1-ethyl-3-methyl imidazolium hexafluorophosphate ([EMIm][PF(6)]), were used for the methanolysis of sunflower oil using Candida antarctica lipase (Novozyme 435) and gave yields of fatty acid methyl esters at 98-99% within 10 h. The optimum conditions of methanolysis in hydrophobic ionic liquids are 2% (w/w) lipase, 1:1 (w/w) oil/ionic liquid and 1:8 (mol/mol) oil/methanol at 58-60 degrees C. Methanolysis using hydrophilic ionic liquids, 3-methyl imidazolium tetrafluoroborate ([HMIm][BF(4)]) and 1-butyl-3-methyl imidazolium tetrafluoroborate ([BMIm][BF(4)]), gave very poor yields. A hydrophobic ionic liquid thus protects the lipase from methanol. Recovered ionic liquids and lipase were used for four successive reaction cycles without any significant loss of activity.

Essential oil composition of two myrtus communis L. varieties grown in North Tunisia.

PubMed

Aidi Wannes, Wissem; Mhamdi, Baya; Marzouk, Brahim

2007-06-01

Two Myrtus communis varieties (var. italica and baetica) were studied in order to investigate their essential oil yield and composition. Essential oil yield varied in leaves, fruits and stems. So, in leaves, it was 0.5% for italica and 0.3% for baetica and was higher than in fruits and stems with respectively 0.1% and 0.04% for italica and 0.07% and 0.03% for baetica. The essential oil analysis performed by GC and GC/MS showed a composition characterized by a high percentage of monoterpene hydrocarbons in leaves, largely due to alpha-pinene with 51.3% for italica and 27.7% for baetica; 1,8-cineole, the alone compound of ether class, was predominant in fruits and stems with respectively 31.6% and 34.7% for italica and 19.8% and 25.8% for baetica.

Process for converting heavy oil deposited on coal to distillable oil in a low severity process

DOEpatents

Ignasiak, Teresa; Strausz, Otto; Ignasiak, Boleslaw; Janiak, Jerzy; Pawlak, Wanda; Szymocha, Kazimierz; Turak, Ali A.

1994-01-01

A process for removing oil from coal fines that have been agglomerated or blended with heavy oil comprises the steps of heating the coal fines to temperatures over 350.degree. C. up to 450.degree. C. in an inert atmosphere, such as steam or nitrogen, to convert some of the heavy oil to lighter, and distilling and collecting the lighter oils. The pressure at which the process is carried out can be from atmospheric to 100 atmospheres. A hydrogen donor can be added to the oil prior to deposition on the coal surface to increase the yield of distillable oil.

Essential Oil Yield Pattern and Antibacterial and Insecticidal Activities of Trachyspermum ammi and Myristica fragrans.

PubMed

Soni, Rajgovind; Sharma, Gaurav; Jasuja, Nakuleshwar Dut

2016-01-01

Two Indian spices, Trachyspermum ammi and Myristica fragrans, were studied for their essential oil (EO) yielding pattern, insecticidal activity, antibacterial activity, and composition. The essential oils (EOs) of T. ammi (1.94 ± 30 mL/100 gm) and M. fragrans (5.93 ± 90 mL/100 gm) were extracted using hydrodistillation method. In Gas Chromatography analysis, the beta-pinene, alpha-pinene, alpha-p-menth-1-en-4-ol, Limonene, and elemicin were found as major constituents of T. ammi essential oil whereas M. fragrans essential oil mostly contains Gamma-Terpinolene, p-Cymene, Thymol, and beta-pinene. The insecticidal activities of EO were demonstrated using LC50 values against Plodia interpunctella and EO of T. ammi was found comparatively more effective than EO of M. fragrans. Further, individual EO and combination of essential oil were examined for antibacterial activity against three Gram (-) bacterial strains (E. coli-MTCC 443, P. vulgaris-MTCC 1771, and K. pneumoniae-MTCC number 7028) and three Gram (+) bacterial strains (S. aureus-MTCC 3381, B. subtilis-MTCC 10619, and B. megaterium-MTCC 2412) by well agar diffusion method. The essential oil in combination (CEO) exhibited higher antibacterial activity as compared with individual essential oils.

[Study on the encapsulation technique of high purity gamma-linolenic acid, part 1--saponification reaction and saponification value].

PubMed

Liu, Feng-xia; Xue, Gang; Gao, Qiu-hua; Gao, Wei-xia; Zhang, Li-hua

2005-03-01

To measure the saponification value and fatty acid formation of evening primrose oil, to study the effects of pH value on production yield and fatty acid formation during the saponification reaction, and to provide rationales for the selection of raw material, the enhancement of production yield of saponification, and the encapsulation of gamma-linolenic acid with urea. To measure fatty acid's formation with gas chromatographic method and to measure the saponification value. The content of gamma-linolenic acid is 7%-10% in evening primrose oil. The content of gamma-linolenic acid is inversely correlated with that of unsaturated fatty acid. The saponification value, the amount of KOH for saponification of evening primrose oil, and the pH value for subsequent isolations of oils are determined. From the measurement of fatty acids of evening primrose oil in two different cultivation locations, the content of gamma-linolenic acid is determined to be 7%-10%, unsaturated oils account for 90%. The saponification value of evening primrose oil is between 180-200, pH value of isolated oil is 1.5-2.0 after saponification reaction. Fatty acids mainly include palmitic acid, stearic acid, oleic acid, linolic acid and gamma-linolenic acid.

Citric acid production in Yarrowia lipolytica SWJ-1b yeast when grown on waste cooking oil.

PubMed

Liu, Xiaoyan; Lv, Jinshun; Xu, Jiaxing; Zhang, Tong; Deng, Yuanfang; He, Jianlong

2015-03-01

In this study, citric acid was produced from waste cooking oil by Yarrowia lipolytica SWJ-1b. To get the maximal yield of citric acid, the compositions of the medium for citric acid production were optimized, and our results showed that extra nitrogen and magnesium rather than vitamin B1 and phosphate were needed for CA accumulation when using waste cooking oil. The results also indicated that the optimal initial concentration of the waste cooking oil in the medium for citric acid production was 80.0 g/l, and the ideal inoculation size was 1 × 10(7) cells/l of medium. We also reported that during 10-l fermentation, 31.7 g/l of citric acid, 6.5 g/l of isocitric acid, 5.9 g/l of biomass, and 42.1 g/100.0 g cell dry weight of lipid were attained from 80.0 g/l of waste cooking oil within 336 h. At the end of the fermentation, 94.6 % of the waste cooking oil was utilized by the cells of Y. lipolytica SWJ-1b, and the yield of citric acid was 0.4 g/g waste cooking oil, which suggested that waste cooking oil was a suitable carbon resource for citric acid production.

Chemical compositions and antimicrobial potential of Actinodaphne macrophylla leaves oils from East Kalimantan

NASA Astrophysics Data System (ADS)

Putri, A. S.; Purba, F. F.; Kusuma, I. W.; Kuspradini, H.

2018-04-01

Essential oils producing plants comprises about 160-200 species, one of which belongs to Lauraceae family. Actinodaphne macrophylla is a plant of the Lauraceae family and widely spread on Kalimantan island. For humans, essential oils are used in cosmetics industry, food industry, and pharmaceutical industry. This research aimed to analyze the characteristics of essential oil and potential of antimicrobial activity from A. macrophylla leaves oils. Essential oils were obtained by steam distillation method. Antimicrobial activity was assayed using agar diffusion method which compared with two synthetic standards including chlorhexidine and chloramphenicol. Four microorganisms were used in this study were Candida albicans, Staphylococcus aureus, Streptococcus mutans, and Streptococcus sobrinus. The obtained oil was determined for its characteristics including the yield, refractive index, and chemical components. The attained components were analyzed using GC-MS. The results of this study showed that essential oils of A. macrophylla leaves contained 0.1051% of yield, clearless, and refractive index was 1.425. Based on GC-MS analysis result, it showed chemical components including spathulenol, 2-monopalmitin, (+)-sabinene, copaen, camphene, and β-pinene. This plant potentially can inhibit the growth of S. aureus, C. albicans, S. sobrinus, and S. mutans with inhibition zones of 17.22, 20.89, 22.34 and 22.89 mm, respectively.

[Ionic liquid based ultrasonication-assisted extraction of essential oil from the leaves of Persicaria minor and conductor-like screening model for realistic solvents study].

PubMed

Habib, Ullah; Cecilia, D Wilfred; Maizatul, S Shaharun

2017-06-08

Ionic liquids (ILs) based ultrasonic-assisted extract has been applied for the extraction of essential oil from Persicaria minor leaves. The effects of temperature, sonication time, and particle size of the plant material on the yield of essential oil were investigated. Among the different ILs employed, 1-ethyl-3-methylimidazolium acetate was the most effective, providing a 9.55% yield of the essential oil under optimum conditions (70 ℃, 25 min, IL:hexane ratio of 7:10 (v/v), particle size 60-80 mesh). The performance of 1-ethyl-3-methylimidazolium acetate in the extraction was attributed to its low viscosity and ability to disintegrate the structural matrix of the plant material. The ability of 1-ethyl-3-methylimidazolium acetate was also confirmed using the conductor like-screening model for realistic solvents. This research proves that ILs can be used to extract essential oils from lignocellulosic biomass.

GC-FID coupled with chemometrics for quantitative and chemical fingerprinting analysis of Alpinia oxyphylla oil.

PubMed

Miao, Qing; Kong, Weijun; Zhao, Xiangsheng; Yang, Shihai; Yang, Meihua

2015-01-01

Analytical methods for quantitative analysis and chemical fingerprinting of volatile oils from Alpinia oxyphylla were established. The volatile oils were prepared by hydrodistillation, and the yields were between 0.82% and 1.33%. The developed gas chromatography-flame ionization detection (GC-FID) method showed good specificity, linearity, reproducibility, stability and recovery, and could be used satisfactorily for quantitative analysis. The results showed that the volatile oils contained 2.31-77.30 μL/mL p-cymene and 12.38-99.34 mg/mL nootkatone. A GC-FID fingerprinting method was established, and the profiles were analyzed using chemometrics. GC-MS was used to identify the principal compounds in the GC-FID profiles. The profiles of almost all the samples were consistent and stable. The harvesting time and source were major factors that affected the profile, while the volatile oil yield and the nootkatone content had minor secondary effects. Copyright © 2014 Elsevier B.V. All rights reserved.

Bio-oil from cassava peel: a potential renewable energy source.

PubMed

Ki, Ong Lu; Kurniawan, Alfin; Lin, Chun Xiang; Ju, Yi-Hsu; Ismadji, Suryadi

2013-10-01

In this work, liquid biofuel (bio-oil) was produced by pyrolizing cassava peel. The experiments were conducted isothermally in a fixed-bed tubular reactor at temperatures ranging from 400 to 600°C with a heating rate of 20°C/min. The chemical compositions of bio-oil were analyzed by a gas chromatography mass spectrometry (GC-MS) technique. For the optimization of liquid product, temperature was plotted to be the most decisive factor. The maximum yield of bio-oil ca. 51.2% was obtained at 525°C and the biofuel has a gross calorific value of 27.43 MJ/kg. The kinetic-based mechanistic model fitted well with experimental yield of pyrolysis products with the mean squared error (MSE) of 13.37 (R(2)=0.96) for solid (char), 16.24 (R(2)=0.95) for liquid (bio-oil), and 0.49 (R(2)=0.99) for gas. Copyright © 2013 Elsevier Ltd. All rights reserved.

Mathematical modelling for extraction of oil from Dracocephalum kotschyi seeds in supercritical carbon dioxide.

PubMed

Sodeifian, Gholamhossein; Sajadian, Seyed Ali; Honarvar, Bizhan

2018-04-01

Extraction of oil from Dracocephalum kotschyi Boiss seeds using supercritical carbon dioxide was designed using central composite design to evaluate the effect of various operating parameters including pressure, temperature, particle size and extraction time on the oil yield. Maximum extraction yield predicted from response surface method was 71.53% under the process conditions with pressure of 220 bar, temperature of 35 °C, particle diameter of 0.61 mm and extraction time of 130 min. Furthermore, broken and intact cells model was utilised to consider mass transfer kinetics of extracted natural materials. The results revealed that the model had a good agreement with the experimental data. The oil samples obtained via supercritical and solvent extraction methods were analysed by gas chromatography. The most abundant acid was linolenic acid. The results analysis showed that there was no significant difference between the fatty acid contents of the oils obtained by the supercritical and solvent extraction techniques.

Synthesis of biodiesel from pongamia oil using heterogeneous ion-exchange resin catalyst.

PubMed

Jaya, N; Selvan, B Karpanai; Vennison, S John

2015-11-01

Biodiesel is a clean-burning renewable substitute fuel for petroleum. Biodiesel could be effectively produced by transesterification reaction of triglycerides of vegetable oils with short-chain alcohols in the presence of homogeneous or heterogeneous catalysts. Conventionally, biodiesel manufacturing processes employ strong acids or bases as catalysts. But, separation of the catalyst and the by-product glycerol from the product ester is too expensive to justify the product use as an automobile fuel. Hence heterogeneous catalysts are preferred. In this study, transesterification of pongamia oil with ethanol was performed using a solid ion-exchange resin catalyst. It is a macro porous strongly basic anion exchange resin. The process parameters affecting the ethyl ester yield were investigated. The reaction conditions were optimized for the maximum yield of fatty acid ethyl ester (FAEE) of pongamia oil. The properties of FAEE were compared with accepted standards of biodiesel. Engine performance was also studied with pongamia oil diesel blend and engine emission characteristics were observed. Copyright © 2015 Elsevier Inc. All rights reserved.

Impact of industrial hammer mill rotor speed on extraction efficiency and quality of extra virgin olive oil.

PubMed

Polari, Juan J; Garcí-Aguirre, David; Olmo-García, Lucía; Carrasco-Pancorbo, Alegría; Wang, Selina C

2018-03-01

Crushing is a key step during olive oil extraction. Among commercial crushers, the hammer mill is the most widely used due to its robustness and high throughput. In the present work, the impact of hammer mill rotor speed on extraction yield and overall quality of super-high-density Arbosana olive oils were assessed in an industrial facility. Our results show that increasing the rotor speed from 2400rpm to 3600rpm led to a rise in oil yield of 1.2%, while conserving quality parameters. Sensory analysis showed more pungency with increased rotation speed, while others attributes were unaffected. Volatile compounds showed little variation with the differences in crusher speed; however, total phenols content, two relevant secoiridoids, and triterpenoids levels increased with rotor speed. Hammer mill rotor speed is a processing variable that can be tuned to increase the extraction efficiency and modulate the chemical composition of extra virgin olive oil. Copyright © 2017 Elsevier Ltd. All rights reserved.

Study of ultrasonic cavitation during extraction of the peanut oil at varying frequencies.

PubMed

Zhang, Lei; Zhou, Cunshan; Wang, Bei; Yagoub, Abu El-Gasim A; Ma, Haile; Zhang, Xiao; Wu, Mian

2017-07-01

The ultrasonic extraction of oils is a typical physical processing technology. The extraction process was monitored from the standpoint of the oil quality and efficiency of oil extraction. In this study, the ultrasonic cavitation fields were measured by polyvinylidene fluoride (PVDF) sensor. Waveform of ultrasonic cavitation fields was gained and analyzed. The extraction yield and oxidation properties were compared. The relationship between the fields and cavitation oxidation was established. Numerical calculation of oscillation cycle was done for the cavitation bubbles. Results showed that the resonance frequency, f r , of the oil extraction was 40kHz. At f r , the voltage amplitude was the highest; the time was the shortest as reaching the amplitude of the waveform. Accordingly, the cavitation effect worked most rapidly, resulting in the strongest cavitation intensity. The extraction yield and oxidation properties were closely related to the cavitation effect. It controlled the cavitation oxidation effectively from the viewpoint of chemical and physical aspects. Copyright © 2017 Elsevier B.V. All rights reserved.

Kinetics of acid base catalyzed transesterification of Jatropha curcas oil.

PubMed

Jain, Siddharth; Sharma, M P

2010-10-01

Out of various non-edible oil resources, Jatropha curcas oil (JCO) is considered as future feedstock for biodiesel production in India. Limited work is reported on the kinetics of transesterification of high free fatty acids containing oil. The present study reports the results of kinetic study of two-step acid base catalyzed transesterification process carried out at an optimum temperature of 65 °C and 50 °C for esterification and transesterification respectively under the optimum methanol to oil ratio of 3:7 (v/v), catalyst concentration 1% (w/w) for H₂SO₄ and NaOH. The yield of methyl ester (ME) has been used to study the effect of different parameters. The results indicate that both esterification and transesterification reaction are of first order with reaction rate constant of 0.0031 min⁻¹ and 0.008 min⁻¹ respectively. The maximum yield of 21.2% of ME during esterification and 90.1% from transesterification of pretreated JCO has been obtained. Copyright © 2010 Elsevier Ltd. All rights reserved.

Transesterification of edible, non-edible and used cooking oils for biodiesel production using calcined layered double hydroxides as reusable base catalysts.

PubMed

Sankaranarayanan, Sivashunmugam; Antonyraj, Churchil A; Kannan, S

2012-04-01

Fatty acid methyl esters (FAME) were produced from edible, non-edible and used cooking oils with different fatty acid contents by transesterification with methanol using calcined layered double hydroxides (LDHs) as solid base catalysts. Among the catalysts, calcined CaAl2-LDH (hydrocalumite) showed the highest activity with >90% yield of FAME using low methanol:oil molar ratio (<6:1) at 65 °C in 5 h. The activity of the catalyst was attributed to its high basicity as supported by Hammett studies and CO(2)-TPD measurements. The catalyst was successfully reused in up to four cycles. Some of the properties such as density, viscosity, neutralization number and glycerol content of the obtained biodiesel matched well with the standard DIN values. It is concluded that a scalable heterogeneously catalyzed process for production of biodiesel in high yields from a wide variety of triglyceride oils including used oils is possible using optimized conditions. Copyright © 2012 Elsevier Ltd. All rights reserved.

Synthesis and analysis of jet fuel from shale oil and coal syncrudes

NASA Technical Reports Server (NTRS)

Gallagher, J. P.; Collins, T. A.; Nelson, T. J.; Pedersen, M. J.; Robison, M. G.; Wisinski, L. J.

1976-01-01

Thirty-two jet fuel samples of varying properties were produced from shale oil and coal syncrudes, and analyzed to assess their suitability for use. TOSCO II shale oil and H-COAL and COED syncrudes were used as starting materials. The processes used were among those commonly in use in petroleum processing-distillation, hydrogenation and catalytic hydrocracking. The processing conditions required to meet two levels of specifications regarding aromatic, hydrogen, sulfur and nitrogen contents at two yield levels were determined and found to be more demanding than normally required in petroleum processing. Analysis of the samples produced indicated that if the more stringent specifications of 13.5% hydrogen (min.) and 0.02% nitrogen (max.) were met, products similar in properties to conventional jet fuels were obtained. In general, shale oil was easier to process (catalyst deactivation was seen when processing coal syncrudes), consumed less hydrogen and yielded superior products. Based on these considerations, shale oil appears to be preferred to coal as a petroleum substitute for jet fuel production.

Utilization of heavy metal-rich tannery sludge for sweet basil (Ocimum basilicum L.) cultivation.

PubMed

Chand, Sukhmal; Singh, Shweta; Singh, Vinay Kumar; Patra, D D

2015-05-01

Unlike food crops, essential oil-bearing crops in which the oil is extracted through hydro-distillation can be a suitable crop to be grown in heavy metal-polluted soils as the oil does not carry any heavy metal. In a field experiment conducted at CIMAP, Lucknow, India during 2011 and 2012, influence of six doses of tannery sludge viz 0, 10, 20, 30, 40, and 50 t ha(-1) were tested, taking sweet basil (Ocimum basilicum) as the test crop. Maximum herb yield was obtained with the application of sludge at 20 t ha(-1). While in root, accumulation of Cd and Pb increased significantly up to 20 t ha(-1), Cr accumulation increased with increasing the dose of tannery sludge reaching maximum at 50 t ha(-1). Essential oil yield of basil (Ocimum basilicum) was significantly affected due to sludge application. Quality of essential oil, in term of chemical constituents, however, was marginally influenced due to tannery sludge application.

Meadowfoam: Pretty flowers, pretty possibilities. [Limnanthes alba

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bosisio, M.

1989-02-01

This article describes the cultivation of oilseed plants that can be used to produce products such as industrial lubricants and polymers. Although native to the Pacific Northwest, the meadowfoam plant (Limnanthes alba) can be grown in mild climates where soil temperatures range from 40-60 degrees F. The greatest deterrent to commercialization is cost due to low yields (nutlets contain 20-30% oil). Plant scientists are working to increase the amount of seed oil yield per acre.

Modeling the initial mechanical response and yielding behavior of gelled crude oil

NASA Astrophysics Data System (ADS)

Lei, Chen; Gang, Liu; Xingguo, Lu; Minghai, Xu; Yuannan, Tang

2018-05-01

The initial mechanical response and yielding behavior of gelled crude oil under constant shear rate conditions were investigated. By putting the Maxwell mechanical analog and a special dashpot in parallel, a quasi-Jeffreys model was obtained. The kinetic equation of the structural parameter in the Houska model was simplified reasonably so that a simplified constitutive equation of the special dashpot was expressed. By introducing a damage factor into the constitutive equation of the special dashpot and the Maxwell mechanical analog, we established a constitutive equation of the quasi-Jeffreys model. Rheological tests of gelled crude oil were conducted by imposing constant shear rates and the relationship between the shear stress and shear strain under different shear rates was plotted. It is found that the constitutive equation can fit the experimental data well under a wide range of shear rates. Based on the fitted parameters in the quasi-Jeffreys model, the shear stress changing rules of the Maxwell mechanical analog and the special dashpot were calculated and analyzed. It is found that the critical yield strain and the corresponding shear strain where shear stress of the Maxwell analog is the maximum change slightly under different shear rates. And then a critical damage softening strain which is irrelevant to the shearing conditions was put forward to describe the yielding behavior of gelled crude oil.

Weed interference with peppermint (Mentha x piperita L.) and spearmint (Mentha spicata L.) crops under different herbicide treatments: effects on biomass and essential oil yield.

PubMed

Karkanis, Anestis; Lykas, Christos; Liava, Vasiliki; Bezou, Anna; Petropoulos, Spyridon; Tsiropoulos, Nikolaos

2018-01-01

'Minor crops' such as spearmint and peppermint are high added value crops, despite the fact that their production area is comparably small worldwide. The main limiting factor in mint commercial cultivation is weed competition. Thus, field experiments were carried out to evaluate the effects of weed interference on growth, biomass and essential oil yield in peppermint and spearmint under different herbicide treatments. The application of pendimethalin and oxyfluorfen provided better control of annual weeds resulting in higher crop yield. Additionally, when treated with herbicides both crops were more competitive against annual weeds in the second year than in the first year. All pre-emergence herbicides increased biomass yield, since pendimethalin, linuron and oxyfluorfen reduced the density of annual weeds by 71-92%, 63-74% and 86-95%, respectively. Weed interference and herbicide application had no effect on essential oil content; however, a relatively strong impact on essential oil production per cultivated area unit was observed, mainly due to the adverse effect of weed interference on plant growth. Considering that pendimethalin and oxyfluorfen were effective against annual weeds in both spearmint and peppermint crops, these herbicides should be included in integrated weed management systems for better weed management in mint crops. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

Co-production of activated carbon, fuel-gas, and oil from the pyrolysis of corncob mixtures with wet and dried sewage sludge.

PubMed

Shao, Linlin; Jiang, Wenbo; Feng, Li; Zhang, Liqiu

2014-06-01

This study explored the amount and composition of pyrolysis gas and oil derived from wet material or dried material during the preparation of sludge-corncob activated carbon, and evaluated the physicochemical and surface properties of the obtained two types of sludge-corncob-activated carbons. For wet material, owing to the presence of water, the yields of sludge-corncob activated carbon and the oil fraction slightly decreased while the yield of gases increased. The main pyrolysis gas compounds were H2 and CO2, and more H2 was released from wet material than dried material, whereas the opposite holds for CO2 Heterocyclics, nitriles, organic acids, and steroids were the major components of pyrolysis oil. Furthermore, the presence of water in wet material reduced the yield of polycyclic aromatic hydrocarbons from 6.76% to 5.43%. The yield of furfural, one of heterocyclics, increased sharply from 3.51% to 21.4%, which could be explained by the enhanced hydrolysis of corncob. In addition, the surface or chemical properties of the two sludge-corncob activated carbons were almost not affected by the moisture content of the raw material, although their mesopore volume and diameter were different. In addition, the adsorption capacities of the two sludge-corncob activated carbons towards Pb and nitrobenzene were nearly identical. © The Author(s) 2014.

Ultrasound assisted intensification of biodiesel production using enzymatic interesterification.

PubMed

Subhedar, Preeti B; Gogate, Parag R

2016-03-01

Ultrasound assisted intensification of synthesis of biodiesel from waste cooking oil using methyl acetate and immobilized lipase obtained from Thermomyces lanuginosus (Lipozyme TLIM) as a catalyst has been investigated in the present work. The reaction has also been investigated using the conventional approach based on stirring so as to establish the beneficial effects obtained due to the use of ultrasound. Effect of operating conditions such as reactant molar ratio (oil and methyl acetate), temperature and enzyme loading on the yield of biodiesel has been investigated. Optimum conditions for the conventional approach (without ultrasound) were established as reactant molar ratio of 1:12 (oil:methyl acetate), enzyme loading of 6% (w/v), temperature of 40 °C and reaction time of 24 h and under these conditions, 90.1% biodiesel yield was obtained. The optimum conditions for the ultrasound assisted approach were oil to methyl acetate molar ratio of 1:9, enzyme loading of 3% (w/v), and reaction time of 3 h and the biodiesel yield obtained under these conditions was 96.1%. Use of ultrasound resulted in significant reduction in the reaction time with higher yields and lower requirement of the enzyme loading. The obtained results have clearly established that ultrasound assisted interesterification was a fast and efficient approach for biodiesel production giving significant benefits, which can help in reducing the costs of production. Reusability studies for the enzyme were also performed but it was observed that reuse of the catalyst under the optimum experimental condition resulted in reduced enzyme activity and biodiesel yield. Copyright © 2015 Elsevier B.V. All rights reserved.

Levels of polychlorinated biphenyls and pesticides in bluefish before and after cooking.

PubMed

Trotter, W J; Corneliussen, P E; Laski, R R; Vannelli, J J

1989-01-01

Similar levels of polychlorinated biphenyls (PCBs), pesticides, and fat were found in 20 correlated uncooked and cooked (baked) bluefish fillets. Fillets averaged 2.5 ppm PCBs as Aroclor 1254 (whole basis) before cooking; after cooking, with the oil drippings and skin discarded, the average PCB level was 2.7 ppm. Although PCBs, lipophilic pesticides, and fat were lost along with oil drippings and skin that were discarded after cooking, the moisture loss in the fillets during cooking compensated for these weight losses almost completely. After the fillets were cooked and the oil drippings and skin were discarded, the PCB content of the fillets was 27% lower on the average.

Generation of aliphatic acid anions and carbon dioxide by hydrous pyrolysis of crude oils

USGS Publications Warehouse

Kharaka, Y.K.; Lundegard, P.D.; Ambats, G.; Evans, William C.; Bischoff, J.L.

1993-01-01

Two crude oils with relatively high (0.60 wt%) and low (0.18 wt%) oxygen contents were heated in the presence of water in gold-plated reactors at 300??C for 2348 h. The high-oxygen oil was also heated at 200??C for 5711 h. The compositions of aqueous organic acid anions of the oils and of the headspace gases were monitored inn order to investigate the distribution of organic acids that can be generated from liquid petroleum. The oil with higher oxygen content generated about five times as much organic anions as the other oil. The dominant organic anions produced were acetate, propionate and butyrate. Small amounts of formate, succinate, methyl succinate and oxalate were also produced. The dominant oxygen-containing product was CO2, as has been observed in similar studies on the hydrous pyrolysis of kerogen. These results indicate that a significant portion (10-30%) of organic acid anions reported i be generated by thermal alteration of oils in reservoir rocks. The bulk of organic acid anions present in formation waters, however, is most likely generated by thermal alteration of kerogen in source rocks. Kerogen is more abundant than oil in sedimentary basins and the relative yields of organic acid anions reported from the hydrous pyrolysis of kerogen are much higher than the yields obtained for the two oils. ?? 1993.

Oily wastewaters treatment using Pseudomonas sp. isolated from the compost fertilizer

PubMed Central

2014-01-01

Background Discharging the oily wastewater in the environment causes serious problems, because of the oil compounds and organic materials presence. Applying biological methods using the lipase enzyme producer microorganisms can be an appropriate choice for treatment of these wastewaters. The aim of this study is to treat those oil wastewaters having high concentration of oil by applying lipase enzyme producer bacteria. Materials and methods Oil concentration measurement was conducted using the standard method of gravimetric and the wastewater under study was synthetically made and contained olive, canola and sunflower oil. The strain used in this study was Pseudomonas strain isolated from compost fertilizer. The oil under study had concentration of 1.5 to 22 g/l. Results The oil removal amount in concentrations lower than 8.4 g/l was over 95 ± 1.5%. Increase of the oil's concentration to 22 g/l decreases the amount of removal in retention time of 44 hours to 85 ± 2.5%. The best yield of removing this strain in retention time of 44 hours and temperature of 30°C was achieved using Ammonium Nitrate as the nitrogen resource which yield was about 95 percent. Conclusion The findings of the research showed that Pseudomonas bacteria isolated from the compost fertilizer can degrade high concentration oils. PMID:24876932

High-sensitivity gas-mapping 3D imager and method of operation

DOEpatents

Kreitinger, Aaron; Thorpe, Michael

2018-05-15

Measurement apparatuses and methods are disclosed for generating high-precision and -accuracy gas concentration maps that can be overlaid with 3D topographic images by rapidly scanning one or several modulated laser beams with a spatially-encoded transmitter over a scene to build-up imagery. Independent measurements of the topographic target distance and path-integrated gas concentration are combined to yield a map of the path-averaged concentration between the sensor and each point in the image. This type of image is particularly useful for finding localized regions of elevated (or anomalous) gas concentration making it ideal for large-area leak detection and quantification applications including: oil and gas pipeline monitoring, chemical processing facility monitoring, and environmental monitoring.

Impacts of climate change on peanut yield in China simulated by CMIP5 multi-model ensemble projections

NASA Astrophysics Data System (ADS)

Xu, Hanqing; Tian, Zhan; Zhong, Honglin; Fan, Dongli; Shi, Runhe; Niu, Yilong; He, Xiaogang; Chen, Maosi

2017-09-01

Peanut is one of the major edible vegetable oil crops in China, whose growth and yield are very sensitive to climate change. In addition, agriculture climate resources are expected to be redistributed under climate change, which will further influence the growth, development, cropping patterns, distribution and production of peanut. In this study, we used the DSSAT-Peanut model to examine the climate change impacts on peanut production, oil industry and oil food security in China. This model is first calibrated using site observations including 31 years' (1981-2011) climate, soil and agronomy data. This calibrated model is then employed to simulate the future peanut yield based on 20 climate scenarios from 5 Global Circulation Models (GCMs) developed by the InterSectoral Impact Model Intercomparison Project (ISIMIP) driven by 4 Representative Concentration Pathways (RCPs). Results indicate that the irrigated peanut yield will decrease 2.6% under the RCP 2.6 scenario, 9.9% under the RCP 4.5 scenario and 29% under the RCP 8.5 scenario, respectively. Similarly, the rain-fed peanut yield will also decrease, with a 2.5% reduction under the RCP 2.6 scenario, 11.5% reduction under the RCP 4.5 scenario and 30% reduction under the RCP 8.5 scenario, respectively.

Biocrude oils from the fast pyrolysis of poultry litter and hardwood

DOE Office of Scientific and Technical Information (OSTI.GOV)

Agblevor, F.A., E-mail: Fagblevo@vt.ed; Beis, S.; Kim, S.S.

2010-02-15

The safe and economical disposal of poultry litter is becoming a major problem for the USA poultry industry. Current disposal methods such as land application and feeding to cattle are now under pressure because of pollution of water resources due to leaching, runoffs and concern for mad cow disease contamination of the food chain. Incineration or combustion is potentially applicable to large scale operations, but for small scale growers and EPA non-attainment areas, this is not a suitable option because of the high cost of operation. Thus, there is a need for developing appropriate technologies to dispose poultry litter. Poultrymore » litters from broiler chicken and turkey houses, as well as bedding material were converted into biocrude oil in a fast pyrolysis fluidized bed reactor. The biocrude oil yields were relatively low ranging from 36 wt% to 50 wt% depending on the age and bedding material content of the litter. The bedding material (which was mostly hardwood shavings) biocrude oil yield was 63 wt%. The higher heating value (HHV) of the poultry litter biocrude oils ranged from 26 MJ/kg to 29 MJ/kg while that of the bedding material was 24 MJ/kg. The oils had relatively high nitrogen content ranging from 4 wt% to 8 wt%, very low sulfur (<1 wt%) content and high viscosity. The viscosities of the oils appeared to be a function of both the source of litter and the pyrolysis temperature. The biochar yield ranged from 27 wt% to 40 wt% depending on the source, age and composition of the poultry litter. The biochar ash content ranged from 24 wt% to 54 wt% and was very rich in inorganic components such as potassium and phosphorous.« less

Successful scaling-up of self-sustained pyrolysis of oil palm biomass under pool-type reactor.

PubMed

Idris, Juferi; Shirai, Yoshihito; Andou, Yoshito; Mohd Ali, Ahmad Amiruddin; Othman, Mohd Ridzuan; Ibrahim, Izzudin; Yamamoto, Akio; Yasuda, Nobuhiko; Hassan, Mohd Ali

2016-02-01

An appropriate technology for waste utilisation, especially for a large amount of abundant pressed-shredded oil palm empty fruit bunch (OFEFB), is important for the oil palm industry. Self-sustained pyrolysis, whereby oil palm biomass was combusted by itself to provide the heat for pyrolysis without an electrical heater, is more preferable owing to its simplicity, ease of operation and low energy requirement. In this study, biochar production under self-sustained pyrolysis of oil palm biomass in the form of oil palm empty fruit bunch was tested in a 3-t large-scale pool-type reactor. During the pyrolysis process, the biomass was loaded layer by layer when the smoke appeared on the top, to minimise the entrance of oxygen. This method had significantly increased the yield of biochar. In our previous report, we have tested on a 30-kg pilot-scale capacity under self-sustained pyrolysis and found that the higher heating value (HHV) obtained was 22.6-24.7 MJ kg(-1) with a 23.5%-25.0% yield. In this scaled-up study, a 3-t large-scale procedure produced HHV of 22.0-24.3 MJ kg(-1) with a 30%-34% yield based on a wet-weight basis. The maximum self-sustained pyrolysis temperature for the large-scale procedure can reach between 600 °C and 700 °C. We concluded that large-scale biochar production under self-sustained pyrolysis was successfully conducted owing to the comparable biochar produced, compared with medium-scale and other studies with an electrical heating element, making it an appropriate technology for waste utilisation, particularly for the oil palm industry. © The Author(s) 2015.

Factors affecting emulsion stability and quality of oil recovered from enzyme-assisted aqueous extraction of soybeans.

PubMed

Jung, S; Maurer, D; Johnson, L A

2009-11-01

The objectives of the present study were to assess how the stability of the emulsion recovered from aqueous extraction processing of soybeans was affected by characteristics of the starting material and extraction and demulsification conditions. Adding endopeptidase Protex 6L during enzyme-assisted aqueous extraction processing (EAEP) of extruded soybean flakes was vital to obtaining emulsions that were easily demulsified with enzymes. Adding salt (up to 1.5 mM NaCl or MgCl(2)) during extraction and storing extruded flakes before extraction at 4 and 30 degrees C for up to 3 months did not affect the stabilities of emulsions recovered from EAEP of soy flour, flakes and extruded flakes. After demulsification, highest free oil yield was obtained with EAEP of extruded flakes, followed by flour and then flakes. The same protease used for the extraction step was used to demulsify the EAEP cream emulsion from extruded full-fat soy flakes at concentrations ranging from 0.03% to 2.50% w/w, incubation times ranging from 2 to 90 min, and temperatures of 25, 50 or 65 degrees C. Highest free oil recoveries were achieved at high enzyme concentrations, mild temperatures, and short incubation times. Both the nature of enzyme (i.e., protease and phospholipase), added alone or as a cocktail, concentration of enzymes (0.5% vs. 2.5%) and incubation time (1 vs. 3 h), use during the extraction step, and nature of enzyme added for demulsifying affected free oil yield. The free oil recovered from EAEP of extruded flakes contained less phosphorus compared with conventional hexane-extracted oil. The present study identified conditions rendering the emulsion less stable, which is critical to increasing free oil yield recovered during EAEP of soybeans, an environmentally friendly alternative processing method to hexane extraction.

Biocrude oils from the fast pyrolysis of poultry litter and hardwood.

PubMed

Agblevor, F A; Beis, S; Kim, S S; Tarrant, R; Mante, N O

2010-02-01

The safe and economical disposal of poultry litter is becoming a major problem for the USA poultry industry. Current disposal methods such as land application and feeding to cattle are now under pressure because of pollution of water resources due to leaching, runoffs and concern for mad cow disease contamination of the food chain. Incineration or combustion is potentially applicable to large scale operations, but for small scale growers and EPA non-attainment areas, this is not a suitable option because of the high cost of operation. Thus, there is a need for developing appropriate technologies to dispose poultry litter. Poultry litters from broiler chicken and turkey houses, as well as bedding material were converted into biocrude oil in a fast pyrolysis fluidized bed reactor. The biocrude oil yields were relatively low ranging from 36 wt% to 50 wt% depending on the age and bedding material content of the litter. The bedding material (which was mostly hardwood shavings) biocrude oil yield was 63 wt%. The higher heating value (HHV) of the poultry litter biocrude oils ranged from 26 MJ/kg to 29 MJ/kg while that of the bedding material was 24 MJ/kg. The oils had relatively high nitrogen content ranging from 4 wt% to 8 wt%, very low sulfur (<1 wt%) content and high viscosity. The viscosities of the oils appeared to be a function of both the source of litter and the pyrolysis temperature. The biochar yield ranged from 27 wt% to 40 wt% depending on the source, age and composition of the poultry litter. The biochar ash content ranged from 24 wt% to 54 wt% and was very rich in inorganic components such as potassium and phosphorous.

Conventional and catalytic pyrolysis of pinyon juniper biomass

NASA Astrophysics Data System (ADS)

Yathavan, Bhuvanesh Kumar

Pinyon and juniper are invasive woody species in Western United States that occupy over 47 million acres of land. The US Bureau of Land Management (BLM) has embarked on harvesting these woody species to make room for range grasses for grazing. The major application of harvested pinyon-juniper (PJ) is low value firewood. Thus, there is a need to develop new high value products from this woody biomass to reduce the cost of harvesting. In this research PJ biomass was processed through pyrolysis technology to produce value added products. The first part of the thesis demonstrates the effect of PJ wood, bark and mixture biomass and temperature on the product yield and on the quality of the bio-oil produced. The second part focuses on the optimization of process parameters for maximum yield and the third part focuses on upgrading the bio-oil with an industrial catalyst (HZSM5) and an industrial waste product (red mud). The results obtained from the first part showed that PJ wood produced maximum bio-oil yield, followed by PJ mixture and bark. The bio-oil yield from PJ wood had low viscosity when compared to PJ mixture and PJ bark. The second part focused on studying the effect of process parameters (temperature, feed rate and the gas flow rate) on the total liquid, organic, water, char and gas yield. The results show that each response is affected by different factor level combinations, and maximum yield for each response was obtained at different factors level. The third part focused on catalytic pyrolysis of PJ biomass using both HZSM-5 catalyst and red mud. The mechanisms of catalysis by the two catalysts were quite different. Whereas the HZSM-5 rejected oxygen mostly as carbon monoxide and water and produced lower amounts of carbon dioxide, on the contrary the red mud produced more carbon dioxide and water and less carbon monoxide. The higher heating value of the red mud catalyzed oil (29.46 MJ/kg) was slightly higher than that catalyzed by HZSM-5 (28.55 MJ/kg). Thus, red mud can be used to achieve similar catalytic pyrolysis results as HZSM-5 catalysts.

Extraction and demulsification of oil from wheat germ, barley germ, and rice bran using an aqueous enzymatic method

USDA-ARS?s Scientific Manuscript database

An aqueous enzymatic method was developed to extract oil from wheat germ. The parameters that influence oil yield were investigated, including wheat germ pretreatment, comparison of various industrial enzymes, pH, ratio of wheat germ to water, reaction time and demulsification. Pretreatment at 180ºC...

[Study on essential oil separation from Forsythia suspensa oil-bearing water body based on vapor permeation membrane separation technology].

PubMed

Zhang, Qian; Zhu, Hua-Xu; Tang, Zhi-Shu; Pan, Yong-Lan; Li, Bo; Fu, Ting-Ming; Yao, Wei-Wei; Liu, Hong-Bo; Pan, Lin-Mei

2018-04-01

To investigate the feasibility of vapor permeation membrane technology in separating essential oil from oil-water extract by taking the Forsythia suspensa as an example. The polydimethylsiloxane/polyvinylidene fluoride (PDMS/PVDF) composite flat membrane and a polyvinylidene fluoride (PVDF) flat membrane was collected as the membrane material respectively. Two kinds of membrane osmotic liquids were collected by self-made vapor permeation device. The yield of essential oil separated and enriched from two kinds of membrane materials was calculated, and the microscopic changes of membrane materials were analyzed and compared. Meanwhile, gas chromatography-mass spectrometry (GC-MS) was used to compare and analyze the differences in chemical compositions of essential oil between traditional steam distillation, PVDF membrane enriched method and PDMS/PVDF membrane enriched method. The results showed that the yield of essential oil enriched by PVDF membrane was significantly higher than that of PDMS/PVDF membrane, and the GC-MS spectrum showed that the content of main compositions was higher than that of PDMS/PVDF membrane; The GC-MS spectra showed that the components of essential oil enriched by PVDF membrane were basically the same as those obtained by traditional steam distillation. The above results showed that vapor permeation membrane separation technology shall be feasible for the separation of Forsythia essential oil-bearing water body, and PVDF membrane was more suitable for separation and enrichment of Forsythia essential oil than PDMS/PVDF membrane. Copyright© by the Chinese Pharmaceutical Association.

In situ impact of petrochemicals on the photosynthesis of the seagrass Zostera capricorni.

PubMed

Macinnis-Ng, Catriona M O; Ralph, Peter J

2003-11-01

We used photosynthetic activity (measured as chlorophyll a fluorescence) and photosynthetic pigment concentrations to assess the effect of pulsed exposures of aged crude oil (Champion Crude), dispersant (VDC) and an oil+dispersant mixture on the seagrass Zostera capricorni Aschers in laboratory and field experiments, using custom-made chambers. Samples were exposed for 10 h to 0.25% and 0.1% concentrations of aged crude oil and dispersant as well as mixtures of 0.25% oil+0.05% dispersant and 0.1% oil+0.02% dispersant. During this time and for the subsequent four day recovery period, the maximum and effective quantum yields of photosystem II (Fv/Fm and DeltaF/Fm' respectively) were measured. In the laboratory experiments, both values declined in response to oil exposure and remained low during the recovery period. Dispersant exposure caused a decline in both values during the recovery period, while the mixture of aged crude oil+dispersant had little impact on both quantum yields. In situ samples were less sensitive than laboratory samples, showing no photosynthetic impact due to dispersant and oil+dispersant mixture. Despite an initial decline in DeltaF/Fm', in situ oil-exposed samples recovered by the end of the experiment. Chlorophyll pigment analysis showed only limited ongoing impact in both laboratory and field situations. This study suggests that laboratory experiments may overestimate the ongoing impact of petrochemicals on seagrass whilst the dispersant VDC can reduce the impact of oil on seagrass photosynthesis.

Variation in the volatile terpenoids of two industrially important basil (Ocimum basilicum L.) cultivars during plant ontogeny in two different cropping seasons from India.

PubMed

Verma, Ram Swaroop; Padalia, Rajendra Chandra; Chauhan, Amit

2012-02-01

Two Ocimum basilicum cultivars, 'Vikarsudha' and 'CIM-Saumya', grown in the Kumaon region of western Himalaya were evaluated for their essential oil yield and composition at different stages of plant growth during two distinct cropping seasons (spring-summer and rain-autumn). The highest yield of essential oil was obtained at full bloom stage in both cultivars in both cropping seasons. The essential oils obtained from different stages in two cropping seasons were analysed by capillary gas chromatography with flame ionisation detection, and gas chromatography-mass spectrometry. The major component of cultivar 'Vikarsudha' was methyl chavicol (84.3-94.3%), while for cultivar 'CIM-Saumya' the main components were methyl chavicol (62.5-77.6%) and linalool (14.4-34.1%). This study clearly indicated that cultivar, cropping season, plant ontogeny and plant part had significant effects on the yield and quality of the essential oil of O. basilicum. Further, the amount of methyl chavicol in the cultivars grown in this region was higher than in cultivars from other parts of India. Copyright © 2011 Society of Chemical Industry.

Biodiesel production from various oils under supercritical fluid conditions by Candida antartica lipase B using a stepwise reaction method.

PubMed

Lee, Jong Ho; Kwon, Cheong Hoon; Kang, Jeong Won; Park, Chulhwan; Tae, Bumseok; Kim, Seung Wook

2009-05-01

In this study, we evaluate the effects of various reaction factors, including pressure, temperature, agitation speed, enzyme concentration, and water content to increase biodiesel production. In addition, biodiesel was produced from various oils to establish the optimal enzymatic process of biodiesel production. Optimal conditions were determined to be as follows: pressure 130 bar, temperature 45 degrees C, agitation speed 200 rpm, enzyme concentration 20%, and water contents 10%. Among the various oils used for production, olive oil showed the highest yield (65.18%) upon transesterification. However, when biodiesel was produced using a batch system, biodiesel conversion yield was not increased over 65%; therefore, a stepwise reaction was conducted to increase biodiesel production. When a reaction medium with an initial concentration of methanol of 60 mmol was used and adjusted to maintain this concentration of methanol every 1.5 h during biodiesel production, the conversion yield of biodiesel was 98.92% at 6 h. Finally, reusability was evaluated using immobilized lipase to determine if this method was applicable for industrial biodiesel production. When biodiesel was produced repeatedly, the conversion rate was maintained at over 85% after eight reuses.

Fuel purpose hydrotreating of sunflower oil on CoMo/Al2O3 catalyst.

PubMed

Krár, Márton; Kovács, Sándor; Kalló, Dénes; Hancsók, Jeno

2010-12-01

The importance of the economical production and usage of new generation biofuels, the so-called bio gas oil (paraffins from triglycerides) and the results of the investigation for their productability on the CoMo/Al(2)O(3) catalyst, which was activated by reduction, are presented. The conversion of triglycerides, the yield of total organic fractions and the target product, furthermore the type and ratio of deoxygenation reactions were determined as a function of process parameters. The advantageous process parameters were found (380 degrees C, 40-60 bar, 500-600 Nm(3)/m(3) H(2)/sunflower oil ratio, 1.0 h(-1)), where the conversion of triglycerides was 100% and the yield of the target fraction [high paraffin containing (>99%) gas oil boiling range product] was relatively high (73.7-73.9%). The deoxygenation of triglycerides the reduction as well as the decarboxylation/decarbonylation reactions took place. The yield of the target fractions did not achieve the theoretical values (81.4-86.5%). That is why it is necessary to separate the target fraction and recirculate the heavy fraction. 2010 Elsevier Ltd. All rights reserved.